LOCUS       DACTJE010000001        72551 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.001, whole genome shotgun sequence.
ACCESSION   DACTJE010000001 DACTJE010000000
VERSION     DACTJE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 72551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72551
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.001"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..563)
                     /locus_tag="I9760_000001"
     CDS             complement(<1..563)
                     /locus_tag="I9760_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT5041804.1"
                     /translation="MINIIKNRNKKNHTASTELKSKIKKEQVKLLFDQVGMAISGEAL
                     TVTILPIALWSVTNHELLIIWMIFTYVFSDLYKSMLLFYYNKQPTLFSTEKWLFLFNL
                     GVVSSGLAWGFASSIMIPASSTLHQIFVVFLITGVTAAANSLYSPVRLSYFLFLVTAF
                     VPFTIWLFSKGQVYLLLGFCGIIYLGI"
     gene            complement(878..1846)
                     /gene="gspK"
                     /locus_tag="I9760_000002"
                     /gene_synonym="lspK"
     CDS             complement(878..1846)
                     /gene="gspK"
                     /locus_tag="I9760_000002"
                     /gene_synonym="lspK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspK family T2SS minor pseudopilin variant LspK"
                     /protein_id="HAT5041805.1"
                     /translation="MFMSSPPSKSIASSKMQGSALLTALFIMTLVAIVATAMSTRLQQ
                     DIYRTRLVIAQDKLYLASQAVTFWALNELLDKNNHFTKTNQLGMVAQYPKNMESIYNQ
                     VQLSGGIFDLQARFNLNNLIEKKSIPTLMHLISHAYSKATGQERANIALGVKHWLLAY
                     DLGRGEDIYTSYYLSQKPPYYPSHQLIKSKSEFRLIKDVSAPAYIALEPFITALPEST
                     SININTAPKQILMSLGDGLSDAQANELITARGENGITDLKEINELLKKFNIPSDQITV
                     ESQYYLSVAYAKNDEFSLVVYSLLKRSRDRKGKLLTSVIRESINNF"
     gene            complement(1833..2450)
                     /gene="gspJ"
                     /locus_tag="I9760_000003"
                     /gene_synonym="lspJ"
     CDS             complement(1833..2450)
                     /gene="gspJ"
                     /locus_tag="I9760_000003"
                     /gene_synonym="lspJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspJ family T2SS minor pseudopilin variant LspJ"
                     /protein_id="HAT5041806.1"
                     /translation="MIKNKGFTLIEILIALTVFAILATITSSTLYYAFNTRTRVNAQA
                     ERLNALQLAISIIQQDTSQTVERAIRGNEMRLFPIFVGQPQYLELTRDGVVNPNSTEK
                     RSTLERVALVCQDSKLLRRTWNTLDPIDRNIYKDKVLLDNLSECHFNYLNQNLQLLSE
                     WREQAVNQNQQREPLPKAIQMNLTLKDWGDMNMLFIIPGALYVYE"
     gene            complement(2447..2824)
                     /gene="gspI"
                     /locus_tag="I9760_000004"
                     /gene_synonym="lspI"
     CDS             complement(2447..2824)
                     /gene="gspI"
                     /locus_tag="I9760_000004"
                     /gene_synonym="lspI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspI family T2SS minor pseudopilin variant LspI"
                     /protein_id="HAT5041807.1"
                     /translation="MMPKTKLVSGFTLIEVLLALTVIAIALTALLKATAQNIDNTHRI
                     KEKTISHWVAMQGIAMIQLNLLRTSQSQESTQATTMLGQKWYWRAKISPTPIKRMQQI
                     TISVSSKQAGPFREELIAFRYLP"
     gene            complement(2821..3306)
                     /gene="gspH"
                     /locus_tag="I9760_000005"
                     /gene_synonym="lspH"
     CDS             complement(2821..3306)
                     /gene="gspH"
                     /locus_tag="I9760_000005"
                     /gene_synonym="lspH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH family T2SS minor pseudopilin variant LspH"
                     /protein_id="HAT5041808.1"
                     /translation="MTTHKGFTLIEILIVIVIIGITLGFALIAFGDFGESRRIQFSAE
                     QLANTLSLAQQQAILESSTLGLKIDNSSYQILKFQDQAGWNPISNKGIFKLYYFPKNV
                     VISLKTNLKHSRGNPAIIIAPSGDMTPFTIEFGTDKEKWIAKLTGSHNGDLDFTMAKS
                     Q"
     gene            complement(3293..3715)
                     /gene="gspG"
                     /locus_tag="I9760_000006"
                     /gene_synonym="lspG"
     CDS             complement(3293..3715)
                     /gene="gspG"
                     /locus_tag="I9760_000006"
                     /gene_synonym="lspG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspG family T2SS major pseudopilin variant LspG"
                     /protein_id="HAT5041809.1"
                     /translation="MNRQKGFSLIEIMVVVVILGILASIVVPKIMGRPDEARKVKAKQ
                     DVLAIQNALDLYKLDNGSYPTTDQGLMALVEKPTSNPVPTNWKQYLKSLPKDPWGREY
                     LYLNPGQHTDVDVFTYGAAGQPGGTGINAEIGNWDDHS"
     gene            complement(3819..5018)
                     /gene="gspF"
                     /locus_tag="I9760_000007"
                     /gene_synonym="lspF"
     CDS             complement(3819..5018)
                     /gene="gspF"
                     /locus_tag="I9760_000007"
                     /gene_synonym="lspF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspF family T2SS innner membrane protein variant
                     LspF"
                     /protein_id="HAT5041810.1"
                     /translation="MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQT
                     LTQQRAASSKSKVSAADLALLTRQLATLLAAGIPVEESLRGVSEQTEKDKVRELIIGV
                     RSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQK
                     VQQALIYPLLMIIVSTAIISFLLTFVVPKIIDVFTESGQTLPPMTKLLINLSQFIKSY
                     GLYSLVTIIVALIGFKKSLSNIKIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILF
                     AAGVSVLETMRVSSSLVTNIVMRQAFDFATLRVREGSGISEALKETKFISPMAIHLIA
                     SGEKSGQLSDMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIF
                     SMEQLVA"
     gene            5115..6524
                     /gene="glnA"
                     /locus_tag="I9760_000008"
     CDS             5115..6524
                     /gene="glnA"
                     /locus_tag="I9760_000008"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAT5041811.1"
                     /translation="MTKNVVLDAIKEHDAKFVDLRFTDIRGKEQHITIPVSAVDDDFL
                     ENGKMIDGSSFKGWQKIHQSDLALVPDLTSIKPDPFFQDNTLFIRCNVVDPQTMMGYD
                     RCPRSIAQRAENYLQSTGIADGVNFGPEPEFFLFDNVQWETTISGSFYKIDSDEAHWN
                     SGKEMEGGNIGHRPPLKGGYFPVPPVDSSHDIRSAICITLESLGMVVEAHHHEVATAN
                     QCEVATRFNTLTKKADELQILKYVIHNVAHNYGKTATFMPKPLVGDNGSGMHCHQSLY
                     KDGYNLFSGDQYSGLSETALYYIGGIIKHARALNAFTNPSTNSYKRLVPGFEAPVLLA
                     YSARNRSAAIRIPHVSNPKARRIEVRFPDPTANPYLAFSAMMMAGIDGIQKKIYPGQP
                     MDKDLYDLPPEELVDVPTVCSSLEMAMDHLRADHEFLLQGDVFSRDFINNYIQMKEEE
                     ITKIRSLTHPYEFELYYSL"
     gene            6537..7097
                     /locus_tag="I9760_000009"
     CDS             6537..7097
                     /locus_tag="I9760_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HAT5041812.1"
                     /translation="MNKLPLFVFLMMVICLSSAQIYKWTDSQGVVHFSDKPHQGAEKI
                     KIPEAQTYTPPANETGGSSEQEQKSVKQEDQEKYTKLEIIQPQNEATIRNNQGYVVVA
                     VQIEPDLSPGDNVQLIFDGAPLGEPQPNLIFQLNGIYRGSHTIAVQVLNDKGDVLKVS
                     DSITIFMHNPRVGMGKGRKKNINSNK"
     gene            7110..7883
                     /gene="yaaA"
                     /locus_tag="I9760_000010"
     CDS             7110..7883
                     /gene="yaaA"
                     /locus_tag="I9760_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAT5041813.1"
                     /translation="MLTLLSPAKKLLSISKHYSKETSNPLLLDKALQLVKIMKLKSVE
                     QIADLMDLSKQLAELNYERYQNFDLKNNPMNHSYPALFLFQGDVYQGLNANSWKDEEI
                     EYAQSHLGILSGLYGFLRPLDRIQPYRLEMGVNLENPAGKNLYAFWSKIVTNILNQIL
                     AEQSNPVLINLASTEYFKVVDEKKLSYPLVTINFYEQKNSELKMIGILAKKARGMMAK
                     YIMQNRIDSIEQIKEFSESGYLFNKEISSPNSLNFIRIH"
     gene            7903..8802
                     /locus_tag="I9760_000011"
     CDS             7903..8802
                     /locus_tag="I9760_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HAT5041814.1"
                     /translation="MWNLSSRVHALNHFPKQGIECYVKRDDELGCGISGTKIRKYSSL
                     IPFLKIKGIRHLIIIAGAQSNNLLAALQVARECQLKVTAFLVKPKHLKIQGNFKLSLL
                     FLHENEIIWINREEWYRVNEFAEQYLEGLRETAYILSEGASVKESMKGAMSLASDIKE
                     NEKILGFAFDHIFVDAGTGFSAIALIKGFYELQHKGFIHVLLLADSEEVFKKKLMHWA
                     GVNPDNYACFYPTTAKSFGSVNQTIKQEIKRLAYEEGILADPIYSAKLFYEARRYIEK
                     YELKGKVLIVHSGGILTMPGFAF"
     gene            9015..10592
                     /locus_tag="I9760_000012"
     CDS             9015..10592
                     /locus_tag="I9760_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase Lgt1"
                     /protein_id="HAT5041815.1"
                     /translation="MKARRDQQLSKLRMRFFSALNHTSEIDLQVLFNDLKSILTLDSI
                     EHLKEGSVAYAIIQELLKQDDAQNKIQSFLHGAIKNVIHPGVIKGLTPDEINWNVAKA
                     YPKYYEHEEFPDVTFGGFKVRDSNEFKFKTNIQTSIWFSIKPDLFMPSKQQEALKRRR
                     EQYPGCEIRLIYSSSLLNAEANRQMKAFARKQNISLIDIDSVKTDSPLYPLLKAELAH
                     LGKGGNPAAASDLCRWIPELFNEGFYVDIDLPVDSSKIVEGHQITGGVPIMLNMGSII
                     SEPIAPHHRRQEAVCMNTDIIAYSNDKRTQKMMDTVARYLKNIYDDPYTALKDTPLAQ
                     TAFFNKCQEERKSIFDLRKGLQDAFRSDSLLQLYDFLGADKFKEVFKLKEAQSKYINE
                     HISEFSEKDLLLNLISDKPSEISQHTLDFVKAKAMYIDIAKEHYSAFYKPLVEEISGP
                     GAIYNALGGAGSFTTTHRRLTGPMLPTTPPRVLQVFCDAHDKGPFVSDNIARWQTNVR
                     DLGVLNREGLSWLPSVG"
     gene            10793..12664
                     /gene="htpG"
                     /locus_tag="I9760_000013"
     CDS             10793..12664
                     /gene="htpG"
                     /locus_tag="I9760_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAT5041816.1"
                     /translation="MVSKQQTMGFQTEVKQMLHLVVHSLYSNKEIFLRELISNASDAL
                     DKLRFLALSNGSLFENDSDLKISIQINEKLQTITISDNGIGLSWEEAVENLGTIAKSG
                     TKEFISQLTGEQAKDSQLIGQFGVGFYSAFIVADKVTVKSRRAGLQPEDGIVWESKGD
                     GEFTIGYEKKSTRGTEITLHLKPENDEFLSDWRIRGIISKYSDHICWPIVMKKLSEEG
                     KESKEFETVNKATALWTLQKSEISEEDYKQLYKHISHDYMDPLTWSHNHVEGKHEYIT
                     LLYIPAHAPFDLWQHEAKHGLKLYVKRVFIMDEATQFLPRYLRFVKGIVDASDLPLNI
                     SREILQDNKQVESIRAACTKRVLSMLEKMATNDKETYQKFWNEFGLVLKEGPIEDFAN
                     KEAIAKLLRFSTTASGSEKQEVSLEEYVSRMKEGQDKIYYITASSYNAAKNSPHLEIF
                     RKKGIEVLLLSDKVDEWLVGYMNEFAGKKLQSISKGKVELGDDETSEQIKEQEKTLEP
                     LIKHIKSVLNERVKDVLLTNRLTDSPACVVADEQDMGLEMQRILQAAGQQVPVSKPIF
                     EINPDHALIKRLHDIQDDNQFELWVTMLFEQAVLAEGGQLDNPADFVNRVNRLLVSS"
     gene            complement(12776..13072)
                     /locus_tag="I9760_000014"
     CDS             complement(12776..13072)
                     /locus_tag="I9760_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAT5041817.1"
                     /translation="MNVIDTQATTQSTKQEQGLQDLVYSLVTRFLAENKSKSINDLYD
                     MILSEVEPPLLQAVMEKRRGNQLQAAKMLGISRGTIRKKLQRYFGTKYFRLTEE"
     gene            complement(13188..14342)
                     /locus_tag="I9760_000015"
     CDS             complement(13188..14342)
                     /locus_tag="I9760_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lipid-A-disaccharide synthase 1"
                     /protein_id="HAT5041818.1"
                     /translation="MPNASRVVIVAGEESGDHHAAELVKQLKAVYPDLEISGIGGKHL
                     RAAGVHLISDLTRYAVTGLTEIIPFLKIFRKAFQDIKQHLSTQKPDLLILVDYPAFNL
                     RLAKYAKKKLGIKIIYYISPQIWAWKGKRIHLIKDCIDKMAVIFPFEKTIYENAGVPV
                     SFVGHPLVKKIAAAKDKHSGRTSLGLPLNEPIIALLPGSRHSEIERHIPILVNTAKLL
                     TLDNPKLRFVVPIAGTINPDKVKAYFSNQNLTVTFIQGQAIECMSAADFVIVASGTAS
                     LECALLEKPMCIIYKSSFLTYVAAMYFIKVKFLGLCNLLANKMMVPEFLQYDCNAIEL
                     SRYISNFHNDPNQAKSMINQLAKLKESLSSSQADCSLFDLVVAELPEKNA"
     gene            complement(14329..15291)
                     /locus_tag="I9760_000016"
     CDS             complement(14329..15291)
                     /locus_tag="I9760_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAT5041819.1"
                     /translation="MNKIRCAVIGVGYLGRFHAQKYQLIPNAELVAVCDVNSTVCEVV
                     SSELNVPAYINYHDLFGKVDAVSIAATTNKHFEIAKFCIQHGIHVLIEKPITETVSQA
                     EELIRLAKQHQVKLQVGHLERFNSARLALDEYLELPLFIESQRLAPFNPRGTDVNVIL
                     DLMIHDIDIIQNIVQSPIKSITAQGAPVLTKAIDIANARISFANHCVANVTASRISFK
                     TERKTRIFQENSYISIDYQNKQLAVFKKGTGEMFPGIPDITRDQREFEKGDALLEEIR
                     AFLNCIENDSVPLVTGEEGRDALKTAEQITALINNNLVERYAEC"
     gene            15512..16087
                     /gene="rnhB"
                     /locus_tag="I9760_000017"
     CDS             15512..16087
                     /gene="rnhB"
                     /locus_tag="I9760_000017"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAT5041820.1"
                     /translation="MNTVLKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKL
                     SPKQRNLLAIRIKEEALAFAYGRAEVEEIDQLNIHHATLLAMRRAVEALPIQPDNVFV
                     DGAFTPQLNIPCKAIVQGDSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGY
                     ATPVHKEALMRLGPCKIHRRSYSPVADLISK"
     gene            complement(16198..17316)
                     /gene="rodA"
                     /locus_tag="I9760_000018"
     CDS             complement(16198..17316)
                     /gene="rodA"
                     /locus_tag="I9760_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HAT5041821.1"
                     /translation="MSRQTTKPVYRFTAKSLHLDFPLLGLILTLIAFGLLILYSASNA
                     NMGMIMRQSMRLLFAFLIMFVLGFIPPHKYKIWTPWIYGVGLSLLIAVMLMGKIGKGA
                     QRWLELGLFRFQPSEIMKLAVPMMAAWFFDRQSHPSSIRSIGIASLIIFIPALLIAKQ
                     PDLGTAIMVTVAGLCVVFLAGIRFKIILLIALLMCSAIPVVWNLMHDYQKQRVYTLID
                     PEQDPLGAGYHIIQSKIAIGSGGLMGKGWLKGSQSHLNFLPEHATDFIFAVSGEEFGF
                     AGGFAIVALIVLISLRSLNIANNAQTTYTRLLSASLAMTFFLSAFVNIGMVMGIIPVV
                     GIPLPLVSYGGTAMVTFLASFGILMSISSHRILFNSLN"
     gene            complement(17313..19166)
                     /gene="mrdA"
                     /locus_tag="I9760_000019"
     CDS             complement(17313..19166)
                     /gene="mrdA"
                     /locus_tag="I9760_000019"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAT5041822.1"
                     /translation="MYRNQSFKNYRAESQHQRFRLNLLIAILIILSLILILRLAFLQI
                     SEFKRYQTLSLKNQMSVIPIAPPRGVILDRNGVLLAENIPVYVLEITPERVKDMKQTL
                     TKLRELIPSITDEDIDSFNKTRLQNRSFVPIPIKLKLSQEEVATFASNQYHFPGVSIK
                     ARLMRHYPLGEITAHALGYVGRINIQELKQVDSTNYRATNFIGKAGIEKYYEDILHGK
                     VGYQMVETDVSGRTLRIINKVNPHSGAKLYLSIDARLQEAAYNALKDKRGAVVVINSR
                     NGEVLAMVSSPSFDPNIFVSGVSKADYKVLSNALQRPLFNRAVRGVYPPASTIKPFVG
                     LAGLDKGFITTTTEIYDPGKYKLPTSSHVYRDWKKTGHGVINFKRAITVSCDTFFYQL
                     GNKMGISNIEDMLVKFGLGQLTHVDLHEEANGVIPSARWKRQTKGVSWYPGDTLISAI
                     GQGFMLATPLQMANATASLSQHGQRFRPHLLIKTVNSDTNEMEEYKPFEEYPVSLRDE
                     ANWDVVIDAMHSVLTSNEGTGYRFGRNPPYPVAGKTGTAQVYSGRQYEKAKYEDIPEH
                     LRDNSLFIAFTPVEKPEIAIAVVVENDTIASTVARKVLDTYYQLYPIKKTP"
     gene            complement(19333..19803)
                     /gene="rlmH"
                     /locus_tag="I9760_000020"
     CDS             complement(19333..19803)
                     /gene="rlmH"
                     /locus_tag="I9760_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAT5041823.1"
                     /translation="MLKITIITLGNKMPDWVNSGVNEYAKRFHDGIQIKLIEIPLLRR
                     NKSSDLARILEKESALTKDALPANARLIALDMLGKSFSSEELALRLTQLQQISSHLCF
                     IIGGPEGLSNEILTLCDERWSLSKLTLPHPLVRIILLESLYRAWSIINNHPYHK"
     gene            complement(19812..20150)
                     /gene="rsfS"
                     /locus_tag="I9760_000021"
     CDS             complement(19812..20150)
                     /gene="rsfS"
                     /locus_tag="I9760_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAT5041824.1"
                     /translation="MLEKNPTLEKLLKSLEDIQAIDIKIIDVHKQTTITDFMVVTSGR
                     SSRHVKSIAQKVLEDMKVAGLPCLSSTGIEHGDWALIDFGDIILHVMQPEFRHFYNLE
                     GLWEEHPGNS"
     gene            complement(20255..21697)
                     /locus_tag="I9760_000022"
     CDS             complement(20255..21697)
                     /locus_tag="I9760_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAT5041825.1"
                     /translation="MEKHPKSLRIYFTTEMWERYGFYAVQSLLALYLALHFKWPDKEI
                     YSLVGSFTALTYLSPLVGGWIADKLIGQKRAILLGAIVLFLSYCMLSLIDNSLALTSS
                     LAGVAVGTGLLKPNISSLLGNEYPVGSTKRESGFTIFYMGITTGIILGTTLPSKFNEY
                     FGWSASFTSASIGLIIAFVVFLFGIHQYKIKDYNPFVLQPGKIISAFVLLVLLWSLSF
                     YILSSPQLANIMFGLVVLFSASYILYSVNRESANQSRQTLVIGLLCIISVIFWAFYFQ
                     MFMSLTLFIARVVEPSFLGIDFPPPYYITVQSIGMLIIGYFLAKKHRSLTMIERGLST
                     GKKFVLAMVFMTLAYSIIAFVSTVVDKSILISPLLIIPAYLMISLAELLLSPVGLSAI
                     TVLADKNKVSTMMGIFFVSLGIGGFLSGKLADLTAIPTGETNIIVLKTLYATAFTQQL
                     SILFIATLGCLVLFAVIKFLLTHIQICEKQ"
     gene            21920..23584
                     /locus_tag="I9760_000023"
     CDS             21920..23584
                     /locus_tag="I9760_000023"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HAT5041826.1"
                     /translation="MNKDKNKRVISAPRGTEIQAKNWLTEAALRMICNNLDPNVAEDP
                     DSLIVYGGLGKAARNWECFDEIVHVLKLLNNDQTLLIQSGKPVGVFTTHEEAPRILIA
                     NSNLVPRWATWEHFNELDKKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVAAAKKHYQ
                     GDLSGRWILTAGLGGMGGAQPLAGTMAGASVLAVECDRQRIEKRLQTKYLDRYTDNLS
                     EALDWINESCRRKKPVSVAVLGNAAEIFPQLVKLGVQPSLVTDQTSAHDPLNGYLPLG
                     WTLEQAVEMRKKSPEEVVDAAKKSMAVQVHAMLEFHNRGIPVFDYGNNIRQMAFEAGE
                     KNAFSFEGFVPAYIRPLFCEGIGPFRWVALSGDPEDIYATDERVKQLIPDAPHLHHWL
                     DMAREKISFQGLPARICWVGLKDRARLALAFNEMVKNKQVKAPIVIGRDHLDSGSVAS
                     PNRETEGMLDGSDAVSDWPLLNALLNCASGATWVSIHHGGGVGMGFSQHAGVVIVADG
                     TEKAAKRLARVLHNDPATGVMRHADAGYQIAKQCAKENSLWLPMES"
     gene            23598..25118
                     /gene="hutH"
                     /locus_tag="I9760_000024"
     CDS             23598..25118
                     /gene="hutH"
                     /locus_tag="I9760_000024"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HAT5041827.1"
                     /translation="MSEQFILQPGQLSLLSIKQILDEELSCVLAENSFELIRASHQTV
                     KKVIDEKKTVYGINTGFGSLANQTISSDCLKELQRNIVLSHACGTGKLLPDDVVALIL
                     LLKINNLSQGYSGVRLELINALIALFNHKVYPCIPSKGSVGASGDLVPLAHLSLPLLG
                     EGEVRHQGQVISAEEGLKLAGLKPLELEAKEGLALLNGLQVSTALALSALFISETLFE
                     TAIISGSLSVDAASGSDVPFDDRIHQIRGHQAQISAASMYRNLLAGSQIRESHRHCNR
                     VQDPYSLRCQPQIMGAILHQMQFVGQTLQVEANAISDNPLVFAEQGDILSGGNFHGEI
                     IAMAADNLALALSEIGGSAERRIALLIDKNFSGLPAFLVRESGLNSGFMIAHVTAASC
                     ASDNKALAHPHSVDSLPTSANQEDHVSMATSAARRLHEMIDNTSTILAIELLAACQGL
                     EFHKPLKTSPQLDKIYQSVRSVVKEYDKDRYFAPDIEKIKKKILDKEFSLLTLTNE"
     gene            25132..26535
                     /locus_tag="I9760_000025"
     CDS             25132..26535
                     /locus_tag="I9760_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HAT5041828.1"
                     /translation="MELMAEYLTLHQQHKNNPYPSLSERKKLLIAIKKILQTEAYTLA
                     EAINKDFTHRSVEETLFLEVFPTIKAINFCLKNMKKWMRKRRRNVSWLFIPAKAYVIP
                     QPLGVVGIMVPWNYPVYLALVPAIYALAAGNRVMIKMSELSPHIGDTLLKLMHAAGLN
                     HSIRVINGDIELSKQFASLPFGHLMFTGSTNVGKMVMKAASDNLIPVTLELGGKSPAI
                     LSPTMNPAYFKRLFMGKLFNAAQTCIAPDYLLIPKGWEDRVEREFRKFINAFYPDLMS
                     NEQYSSIISERHKKRLLDLVEDARSKGARVVEFGHSIPNCSKLPVFLLFDITNDMLVM
                     KEEIFGPILPVLTYNSINDAVDYINSSPNPLALYYFGEDKSEIKIIQTKTLSGALTIN
                     ETLMHIAIDDLPFGGVGHSGMGHYHGKEGFDTFSKLKPVLVQRFISTVSWLYPPYGAL
                     MRMFLAWVGGIKLKEKS"
     gene            26532..27341
                     /locus_tag="I9760_000026"
     CDS             26532..27341
                     /locus_tag="I9760_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAT5041829.1"
                     /translation="MSKVIFITGGSRGIGREIALKFAAKDKANIVIAAKTAEPHPKLE
                     GTIYSVANEIEELGGKALPLMVDVRDEQQIQNAITKTIETFGRLDVLVNNASAINLTD
                     TLNTPMRRYDLMQSVNVRATFACSQAAIPYLLKSENPHILTLSPPLNMDKTWFAPHLA
                     YTISKYGMSMCTLGLAEEFKEAGIAVNSLWPKTTIATDAIRVHFPSELYMASRKPLIM
                     ADAAYWIINQPSKSITGNFFIDEDVLKNSGVTDFSCYAMNPKAQLCPDLFL"
     gene            27338..27769
                     /gene="rnhA"
                     /locus_tag="I9760_000027"
     CDS             27338..27769
                     /gene="rnhA"
                     /locus_tag="I9760_000027"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAT5041830.1"
                     /translation="MKVEIYTDGACKGNPGPGGWGVLLRYNGREKTLHGGEAQTTNNR
                     MELMAAIKGLEALKRPCEVDLYTDSQYLQQGMKEWIKTWKRNGWRNSKKELVKNAELW
                     KSLDNLASIHNIHWHWVKGHSGHLENDLVDALANLGIEELS"
     gene            27774..28475
                     /gene="dnaQ"
                     /locus_tag="I9760_000028"
     CDS             27774..28475
                     /gene="dnaQ"
                     /locus_tag="I9760_000028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAT5041831.1"
                     /translation="MRQVILDTETTGIGPEQGHRVIEIGCIELIDRKLTGKHFHIYLN
                     PQREVDEGAFRVHGISTEFLQDKPLFQDIVTEFIQFVEGSELIIHNAPFDVGFLNSEL
                     KHVKWNKTLEDYCRILDTLILAREKHPGQRNSLDALCKRYEIDHFNRELHGALLDAEI
                     LAYVYLAMTGGQSSLFTEVESSLSQSKIKTQEIKSLSLKKPVIMKANEEELMQHQSFV
                     EFISKKSGINHWEEV"
     gene            complement(28682..29059)
                     /locus_tag="I9760_000029"
     CDS             complement(28682..29059)
                     /locus_tag="I9760_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041832.1"
                     /translation="MSRKLLIATTLVLSTSLFPLISNAEDTANPNEMTKDAWLNSMTP
                     LLPDLICKGFIQDPDLKKRFDEIKMTYEQCVTLIPESTKKCQDELYASMPDKINSETA
                     GTWGRSLGECIGKDFAEKHLIPK"
     gene            29256..29729
                     /locus_tag="I9760_000030"
     CDS             29256..29729
                     /locus_tag="I9760_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAT5041833.1"
                     /translation="MEKSNFRLNFLSQILFFTLGAFLFCNGYAAGNTFIFNPNTLTWK
                     AISSNGKVIRTGRGSGGKHYCSDVRRSCKTPSGVFRVISKGGPACRSSRYPLGKGGAP
                     MPYCMFFSKLYAIHGSPDVPNYNASHGCVRVKPHDARWLSQNFIKIGTKVIIKSY"
     gene            29841..31250
                     /locus_tag="I9760_000031"
     CDS             29841..31250
                     /locus_tag="I9760_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041834.1"
                     /translation="MIEVNIWLSTAFLFKKRIKHKFFGPLLASEDKGENVGHVNFTIE
                     IDERTKTKNSFDFIEQHGSELRAKKTLRVVPTKATNTSPPNIESSHLIPTIVRSDIVS
                     HSFWPDARPTKSETIKGKAVKPKFKTHEEDMISEDSVTAMTIIHRKSALDEISREKME
                     DLDFLVEISDLEVNLEQRKLFMDDLKKLQLEKDELIKQQKQLTNSYQTQLKDLKGQLA
                     KLELHLNKTNGQITATERTINYLNKLKNPDPKTKTQLIELTEKLIKLKQEFETTINSQ
                     HEISALISKSELTYHEDLQQVEKKLQQIELAMNHRIEDLKKLDIKINGRDENDLKELQ
                     EEARRRKEYTSRKEQFIKSRDFTEGRQPDYSVTLPTAESGLTYYVDEIKILKAMQEER
                     SQNYSFLFNNCASSAKRCLLAGIDEKLRAKLKETGLGSKFFEISKVETCKSLRDWART
                     LDSKLTELNLRSPAPTRGA"
     gene            complement(31316..31708)
                     /gene="erpA"
                     /locus_tag="I9760_000032"
     CDS             complement(31316..31708)
                     /gene="erpA"
                     /locus_tag="I9760_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAT5041835.1"
                     /translation="MANLDVSQNTSDIHFSVSAADKVAELIKEEDNSNLNLRVSITGG
                     GCSGFQYGFSFDEQINDDDTIVIQQCSDGKSSVKLLVDSMSYQYLHDAEIDYIKGIQG
                     EQFVIRNPNAKTTCGCGSSFSIGDEDDL"
     gene            complement(31844..32929)
                     /gene="mnmA"
                     /locus_tag="I9760_000033"
     CDS             complement(31844..32929)
                     /gene="mnmA"
                     /locus_tag="I9760_000033"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAT5041836.1"
                     /translation="MKAKVIVGMSGGVDSSVAAWLLKEQGYQVEGLFMKNWEQDDHND
                     YCPAAKDLADAQAVCNQLRIPLHTVNFSKEYWDRVFAYFLSEYEKGRTPNPDVLCNKE
                     IKFNAFLNHALTLGADYIATGHYAKNTIEGSIGYLFKAKDREKDQTYFLHAVEPEALS
                     KTIFPIGDFTKPQIREFAKQLGLVTHAKKDSTGICFIGEKRFKTFLNEFILAKPGEIK
                     STGGKTLGQHDGLMFYTLGQRQGLGIGGLQNSTDEPWYVVDKDIASNTLYVAQGSQHP
                     MLYSQGLICGPIHWLADYENHLPLTCFAKTRYRQTDQACMISPPDNNQHYVMFSSPQR
                     AITPGQFIVFYEKNQCLGGATIEQIIR"
     gene            33202..33630
                     /locus_tag="I9760_000034"
     CDS             33202..33630
                     /locus_tag="I9760_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HAT5041837.1"
                     /translation="MMHLQELAKHGQQEKEVILNERLPSFVSPGCHLHASYHIEAKDD
                     FYLIHLRVQGNLNSVCQRCMENFTVCYNNSTVIAVCRNDERAEQLLSQYECLVSSNWQ
                     VDLNELIIDELHLYAPQVHPDINECANEINEILTGKKQTS"
     gene            33716..33907
                     /gene="rpmF"
                     /locus_tag="I9760_000035"
     CDS             33716..33907
                     /gene="rpmF"
                     /locus_tag="I9760_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAT5041838.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTKPTLSVDPTTGETHLRHHMTP
                     DGYYRGKKIIDAETAYEQE"
     gene            33913..34941
                     /gene="plsX"
                     /locus_tag="I9760_000036"
     CDS             33913..34941
                     /gene="plsX"
                     /locus_tag="I9760_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAT5041839.1"
                     /translation="MKNITIAIDAMGGDHGLEIVIPACIRAIKNNPDLKLLLVGVQDK
                     ISASLKKHGMLSCQQFTIVHASEVVTMDELPSHALRNKKDSSMRIAINLVKEGRAQAC
                     VSAGNTGALMATARYVLKTLPGIDRPAIVSELPTMGGKTRVIDLGANVDSCAEHLFQF
                     AVMGSALIQAIENKPKPKIGLLNIGVEEIKGNDQVKRTAHMLAECSVMNYVGYVEGDH
                     FYSGDVDLVVCDGFVGNVALKASEGLAKLLLTVLKESFSRNWLTKIAGLIALPALKHL
                     KNRLDPSRYNGASLLGLNGIVVKSHGGANEVGFQHAIEQAVLEVKNNVVDLVRDQIND
                     FINQGLLL"
     gene            34938..35891
                     /locus_tag="I9760_000037"
     CDS             34938..35891
                     /locus_tag="I9760_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAT5041840.1"
                     /translation="MKNAVINGTGSYSPERQMTNAELETMLDTSDEWIVTRTGISSRS
                     VAQEHETTSYMASRAAEQALEASGLDAEEIDLILVATCTPDYFFPSVACHVQHALGIK
                     RPIPAFDIGAACSGFVYAMDVAKQYIATGAAKHVLVVGSESMSRAVDWTDRSICVLFG
                     DGAGAVVLSASDRQGIMGSVLHSAYDSDKLLVLRNSTFEQDRATIGMRGNEVFKIAVN
                     IMGNIVDEVLEASHLKKSDIDWLIPHQANIRIIQAIAKKLSLPMSHVIVTIGNQGNTS
                     AASIPLALDYSIKNNQIKRDEILLIESFGGGMTWGAMVIRY"
     gene            35918..36865
                     /gene="fabD"
                     /locus_tag="I9760_000038"
     CDS             35918..36865
                     /gene="fabD"
                     /locus_tag="I9760_000038"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAT5041841.1"
                     /translation="MVKSAFVFPGQGSQSIGMLSDFMPQYTVVTDAFAEASDALGYDL
                     WDLVQNGPETKLNQTEYTQAAMLTADVAIYRLLMQLGVPQPQVMAGHSLGEYAALVCA
                     NSLSLKDAAQLVARRGQVMQNAIPLGEGAMAAIVGLSDEQVRSLCEQASTANHLVTPA
                     NYNALGQIVVAGHRVAVDKLIQLAEEAGARLAMIIPVSVPCHCPLLKNAAELFAEDLA
                     QIEFQVPSVDVISNVDLSIYHSTQHIRDKLKEQLYSPVRWVETVQLIQQRGIDLVVEC
                     GPGKVLNGLIKRIERNLTTISVYDTISLDQVIERLHIPV"
     gene            36877..37623
                     /gene="fabG"
                     /locus_tag="I9760_000039"
     CDS             36877..37623
                     /gene="fabG"
                     /locus_tag="I9760_000039"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAT5041842.1"
                     /translation="MINLEGKIALVTGASRGIGRAVAEKLARNGAFVFGTATTEQGAA
                     SINSFFEKEKLAGKGIVLDVTSTEQVENLMSTLSDNDQCPSILVNNAGITADNLLLRM
                     DDDEWYKVIETNLNSIYRMTKACLKPMFRARWGRIISIGSVVGASGNSGQVNYTAAKA
                     GVIGFSKSLAQEIGSRGITVNVVAPGFIDTDMTTALPDMVKEEMLKRIPMRKLGQPED
                     VADAVAFLASDSAKYITGETIHVNGGMYMS"
     gene            37708..37956
                     /gene="acpP"
                     /locus_tag="I9760_000040"
     CDS             37708..37956
                     /gene="acpP"
                     /locus_tag="I9760_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAT5041843.1"
                     /translation="MSTVEERVRKIVVEQLGVKEEELKNDASFVDDLGADSLDTVELV
                     MALEEEFETEIPDEKAEKITTIQEAIDYIESNLNKEEA"
     gene            37976..39214
                     /gene="fabF"
                     /locus_tag="I9760_000041"
     CDS             37976..39214
                     /gene="fabF"
                     /locus_tag="I9760_000041"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAT5041844.1"
                     /translation="MNSRRVVITGMGMLTPVGLNVEETWRSILAGVSGVGLVEDFDTT
                     EYPTKIWAKVKNFNVENHMPIKDARKMDVFTQYGMAAADEAMLDSGLKIDEVLSRRIG
                     VAVGAGIGGIQTITNNQDKLVAGGPRKVSPFFIPAGIINMVAGQISIKHNLKGPNISV
                     VTACTTGTHNIGLAGRIIAYGDADVMICGGAEMTTTPLCLAGFSAVRSLSKRNDEPEK
                     ASRPWDKDRDGFVMGEGAGVLVLEEYEHAKARGAKIYAELVGFGMSGDAYHITAPDED
                     ADGASRAMEAAIQDAGIDPRQVDYINAHGTSTYLNDLNETKAIKRVFQNHAYDLAVSS
                     TKSMTGHLLGAAGAVEAIISILAIRDQVAPPTINLDNPDEGCDLNYVAHVPQSRTINY
                     VLSNSLGFGGTNGSLLFKRI"
     gene            39215..40213
                     /gene="mltG"
                     /locus_tag="I9760_000042"
     CDS             39215..40213
                     /gene="mltG"
                     /locus_tag="I9760_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAT5041845.1"
                     /translation="MQFSRHKKLVILVLSLFFMSFLFLFWNIYRLLYTPIIPKTASPM
                     ILALDKATSAYQFAYILQDKKLIHSAKFFLLIIRFEGLSHQLKAGVYQITPGETAMKL
                     LHRVVAGDVITQNFTIIEGSTQQKVDYDLRQANYLKYNPEDWAIVKENYPSAEGLLLA
                     DTYQYQGGSSSRALLEQAHRNLLNYLNTSWANRSPNLPYKTPYELLIAASIIEKETAI
                     AQEKKLISGVMVNRLKKKMPLQMDPTVIYGLGNQYKGKLTHNDLLIQSPYNSYLNRGL
                     PPTPIAMVGKEAIDAAAHPQLSNYLYFVAKGDGTHQFSETYEQQRQAINQYRRKDY"
     gene            40213..40851
                     /locus_tag="I9760_000043"
     CDS             40213..40851
                     /locus_tag="I9760_000043"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAT5041846.1"
                     /translation="MSSLTGKLIVIEGLEGAGKSTAVNLVVELLSQKKISTITTREPG
                     GTKIGEILRSIIKNPEYNNVLDDRSELLLLYAARIQLIEQVIKPALNVGQWVIADRFE
                     LSTLAYQGGGRKMDMRVINELSNFCLKGFKPDLTLYLDINPELGMIRAKSRGKFDRIE
                     QESIEFFHRIHDTYHILVKQNPEIIMIDANRSLEEVQSSIQSVIEEFIEHNL"
     gene            40848..41753
                     /locus_tag="I9760_000044"
     CDS             40848..41753
                     /locus_tag="I9760_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAT5041847.1"
                     /translation="MITYQEQWQKMQMALAIRRIPQAMLFSGSLHCGLIEFTNQVIKL
                     LLCKIKQNEPCLTCMDCQMIERFEHPDVEWIKPERSGGIIKIDQIRGLQSTAFLTPQR
                     TNHKLIIIESAESMNIAASNALLKILEEPAQHTIFILLAQQIGNIPPTVLSRCQITQF
                     AVFDELPCNNLLALGEYYTQELNSTMVFKHSESILEGLIAIIERREHPCTLAAQWIQH
                     ELSAFLWFLYLIYSQLQYMHLFRVAYSGTLTKQLNKLSSLLNPVMIFAQVDKINTLLR
                     KLSHNMNINHTLVLEDLLFSLVVDY"
     gene            41880..42221
                     /locus_tag="I9760_000045"
     CDS             41880..42221
                     /locus_tag="I9760_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HAT5041848.1"
                     /translation="MTEKPQIINCSFPNEASLYMAYMPFVKGGGLFIRTQHVYSLGQS
                     LELSVKLLNENEPYLVTGKIVWITPKGAQGNKPPGVGIQFTGENSRYLCNKIETYLAG
                     MLKSCQVTDTI"
     gene            42277..43065
                     /locus_tag="I9760_000046"
     CDS             42277..43065
                     /locus_tag="I9760_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAT5041849.1"
                     /translation="MLVDSHCHLNFIDLTHFNHDLAQVINQARENGVEYFLSVCVELS
                     DYPQLERLAKDYPDISISVGVHPNSEMNYPVTAQMLCELAQNPACIAIGETGLDYYRN
                     PTEEAQSLQRDRFREHIKAALITSKPLIIHTRQAAEDTLLMMAKEGASQIGGVMHCFA
                     EDFDIAQRAIDLNFYISFSGIVTFKNAVSLQDVARQIPLERILIETDSPYLAPVPHRG
                     KQNHPALVKHVAETIAQLRGMDFQEIAEITTNNFYNCFNIKRQG"
     gene            43177..44277
                     /locus_tag="I9760_000047"
     CDS             43177..44277
                     /locus_tag="I9760_000047"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAT5041850.1"
                     /translation="MSLYIGLMSGTSMDGIDAALLELPSNHLIHGITKQYSDDVRRNL
                     DDLIMGNHLTLASICQLNTLIGREFAEAVRQLLSEIKVHPKEIQAIGSHGQTVCHDTS
                     GNIPYTLQLGCGHTISSLTGITVVADFRTRDLVNGGQGAPFAPLYHQQIFSKVNESVA
                     VVNIGGIANVTFIAKNQMTRGWDIGPGNCLMDAWIYKNKGALFDKSGVWASQGEVIYP
                     LLEYLLQDPFFHLDSPKSIGKEYFSLSWLQKHLKPDYTPADIQATLLALTAHTIAETI
                     LNESGEIKQLYLCGGGAHNTHLKENLARLLPGIAVKSIAELGISPDYLEAMMFAWLAA
                     QTINQIPVNLTSITGAKGIAILGAVYPIIKSY"
     gene            44333..45625
                     /locus_tag="I9760_000048"
     CDS             44333..45625
                     /locus_tag="I9760_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5041851.1"
                     /translation="MTAHYNVASNSGARNTLATSYFIFFLAASFYLYEFILQVAPSVM
                     AESMMKTFGVTGQGFGVISAFYFYAYAPTQLPAGVLYDRYGPRKLMTFAIVLCAFGSA
                     FFASTDSVFTACIGRFLIGIGSAFSFIGVLVLISRWFPPYYFAILAGVAQLMSSVGAM
                     FGEMPLAALIDLVGWRSASFILAGVGFLLAILFWVFIRDYPHQQNQTIPDHYLRDEWK
                     RLVAVCKHAYTWIIGGYAFAIWTPIAVFAALWGVPFLQEKFQVSVVAASGLCSMIWLG
                     IGVGSPLLGWLSDKLESRRIALIISAVLGLIATLIILYWSELSYNWAYFVLFVLGLGA
                     GGQTVSFAVVKENNTPELVGTASGFNNLSVLIGGAIFQPLVGYILQQTGSWRIVNGAH
                     VYSISSYQTALLVMPVCFLASLLIATFLLKESHPARRD"
     gene            complement(45701..46981)
                     /locus_tag="I9760_000049"
     CDS             complement(45701..46981)
                     /locus_tag="I9760_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5041852.1"
                     /translation="MSEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKS
                     FNAHESVVATSLTVYVLGGASLQLILGPLSDAYGRRPMMLIGSCLFFLFTLLIASSHS
                     MNQFLIARFFQGMGLCFIGVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGA
                     IIIHYTSWRLIFIIIALGALLAYWGLWRFMPEPIGQVKRDGQLIPKTPFALNAITRNY
                     KTLLSNQAFCYSAIAEGLVGIPCIAWIALAPIILIAEAKLTVIQYGLWQLPIFGATIL
                     GNWCLHHLTYKYKIERIIFIGCIIMVVGLALTALLPYFYGNNYVYLIPGIIIYFFSLS
                     VINAPLNRYCLFVTAVSKGTASALISLSIMIIGAIGIEIANLFYQHHNNLHFAFYCNA
                     VGLLFLIFIGLTFFIGTPKKSEIHDEANSNVANS"
     gene            47212..48378
                     /locus_tag="I9760_000050"
     CDS             47212..48378
                     /locus_tag="I9760_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT5041853.1"
                     /translation="MPKEKVVIIIPTYNEEDNISNTIDLILKETYSLADYQVDILVFD
                     SHSTDSTAHIVRELTYTSQRIHFAQEPQKTGLGSAYLQAMRIAIDELNADVVFEFDAD
                     GSHQPCYIAPMLELLKQHDVVVGSRYISGGRIPSNWAFHRKLLSVGGNWVARMLLTGK
                     YKDLTSGLRAIRTNLLKKLLPSNFLSNNYAYKLELYWLLHKAKARIVEFPIQFIDREK
                     GKSKLPKNSMIDTLRVLFTLRFRELRQYLTMCCVGSIGALIQFSLYNWLRSSNHSALV
                     SSQVAISAAIISNFIFHHSITFKQKTDFRITKTTFSRFTQFIIYSTFMVYFQSLWVHI
                     FVHSFGQGVLKENIIVASGLGIGSIINYLFYSNIIWSSKGTHSLNSNSSKETQI"
     gene            complement(48415..49800)
                     /locus_tag="I9760_000051"
     CDS             complement(48415..49800)
                     /locus_tag="I9760_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HAT5041854.1"
                     /translation="MFLHMNRYISLYFLLILLIAPIHLLGLDSYYYWDWSRHLALSYY
                     DGSPMIAYFIRIATLLFGDTLFALNLVTIVSTALTSFIIYKTARLCLNKEASYVTVLI
                     WLFSPLVTLDLINQTTYDTPLTLFWALTLYFTIRYIQFNRNQDLYYIGLSIGLMLLSK
                     YSGIVLVLALLVFLVATPYRRLFKSRHFYLALTLAIIVFSPVILWNAQHDWVSIRYQL
                     STHQSQNTHHAMGGILHAFFNFFLPAINFLFIPPALYLSKGTPRKSPVFYLCLIICVT
                     FVLFYLFVAGKATIRAYWLSQYIITASLLAGYCYQEWAYRNSTYLLIVFYVLTSGIIL
                     MNNTTLFSFGYSKKLAYYQSIQQINQSNDKLPSIVITPGWFEARMLFFLKNKPTIFTF
                     DCGSMQNQYRYWSPNLNTLGEVLYIDTFDRLSCVKKYFAQCQSLNLPAAFDEKGKPII
                     YAYWCSNKLDT"
     gene            49899..51041
                     /locus_tag="I9760_000052"
     CDS             49899..51041
                     /locus_tag="I9760_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAT5041855.1"
                     /translation="MKLYFFGDDNNKPKLSEHLIIYILSLLDPKSQSVASLVNKDWSR
                     LVQLTRQYLSMLPVIHRIPLFANLGLDVLKLKLMTGGMTNSTVRLRIKNAPEKWVMRI
                     PGIGSSAFITRRDEAHNAKQAEKLGLNVPIAFFDPEDGLQVTRFIEGVHSLDDKALAR
                     KEILHEVASMMRRLHKSPPFDNNTLFFERNEELLDLLKRKPFNFPGEIEFVEQQMKNL
                     KEIFSSYHIQQFPCHNDTTPLNFILSENPEIKGSEKIHQIDWEYSSNNDFIWDLVYFM
                     VEAKLNREQQLILLKAYFNTEELSDSLLAWIEVYKPIIEWWITIWSWTQLANGANAVD
                     LSSYEQLGLERYHNTLSHLKSEDYVKALALIEADSHSLTFTGQRPF"
     gene            complement(51094..51864)
                     /locus_tag="I9760_000053"
     CDS             complement(51094..51864)
                     /locus_tag="I9760_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HAT5041856.1"
                     /translation="MTILVFDIETIPDIESGRRLFALDGLSDEDTACAMFALRREKAG
                     NDFLPHYLQKICAISVVISHGNQIKVWSLGDENSDEKELITRFFAGIDKHTPTLVSWN
                     GSGFDLPVLHYRSLYHSISAPTYWETGENQQTFKWNNYLSRFHYRHIDLMDVLAGYQN
                     RAFAPLDDISTMLGFPGKMGMSGSKVWEQYQAGQLKEIRDYCETDVLNTYCVYLRFEL
                     MRGTISQEGYDRNIAQLKNYLGAEKDKMHLQEFLKKML"
     gene            52110..52706
                     /locus_tag="I9760_000054"
     CDS             52110..52706
                     /locus_tag="I9760_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041857.1"
                     /translation="MKIRFTLLCFSIFLTGCVSKPPADINNICNIFKQYPKWYVDSKD
                     VERRWKVPVPVQMAIIHQESKFNARARPPRTKLLWIIPWTRPSTAYGYTQALRSTWDN
                     YKRTNGWFWSSRDDFGDGVDFIGWYANQANIKAGIPRTDAYSLYLAYHEGIGGYQRKT
                     YLKKPWLISVARKVKAKSQLYAMQLNSCKDSLKSRSWW"
     gene            53101..53757
                     /gene="adk"
                     /locus_tag="I9760_000055"
     CDS             53101..53757
                     /gene="adk"
                     /locus_tag="I9760_000055"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAT5041858.1"
                     /translation="MRLMLLGGPGAGKGTQASLLINRYKIPQISTGDMLRAAIAKGTP
                     LGLSAQKIMESGGLVSDDIIIGLVKERLKNPDCDRGFLFDGFPRTLVQAEALKDAEIH
                     LDHVIEIAVDDEEIIERISGRRIHQPSGRVYHVVNQPPKNPGVDDITGEPLIQRDDDK
                     EETIRKRLQVYHSQTAPLVQYYKEWAESGSKEAPKFHTISGTGTVEQIFDNIVTILET
                     "
     gene            53766..54128
                     /locus_tag="I9760_000056"
     CDS             53766..54128
                     /locus_tag="I9760_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAT5041859.1"
                     /translation="MPTQTINSAEFESLINDNEIVFIDFWAEWCAPCKQFSKVYEQVS
                     DQFPAIKFVKVNIEEQQELADFFEIRSIPHLMVFKDSIVIYSDAGSIPESTLKELAQQ
                     ALDADVSEIRAQLEQKDK"
     gene            complement(54088..55608)
                     /gene="glpD"
                     /locus_tag="I9760_000057"
     CDS             complement(54088..55608)
                     /gene="glpD"
                     /locus_tag="I9760_000057"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAT5041860.1"
                     /translation="MDQVFDVAIIGGGINGCGCAADAALRGLSVILFEQDDLASKTSS
                     SSTKLIHGGLRYLEHYEFGLVKKALQERQTLLNLAPHLVHSQSFVLPYLKHMRPSWLL
                     RLGLFFYDHLSRKNHLPKSRSIHRNNKNNYFTPLKEELNRGFLFYDATTDDARLTILN
                     AIQAKNHGASIRPHAKVTHAEVINNIWHLTIQPTKGQSYKAYAKSIINATGPWVQSIA
                     QLTHTPIQKDITLVKGSHIIVPKLFEGNHAYFLQHNDQRVIFVIPYHGFSMIGTTDIH
                     YTGDLNHIEIGEEEIDYLIELVNAYFKFKITKEDVVHSWSGIRPLLADDSKEVKSLSR
                     DYSYEFNHSPAPIITIFGGKITTYRQLAEEVVDQLTPVFPQMKPSKTQHTPLPGATLA
                     DMSFTQYVNYAKTKYHWLDDELLNRYLNTYGCYTEIFLSQCNNMEAMGKKYGPSLYQV
                     EVDYLILEEWANYCDDILNRRTKLGLTMDNASKKELADYLASISTYPSAQAELVFH"
     gene            55670..57145
                     /gene="glpK"
                     /locus_tag="I9760_000058"
     CDS             55670..57145
                     /gene="glpK"
                     /locus_tag="I9760_000058"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAT5041861.1"
                     /translation="MNYILAIDQGTSSTRAMIYTLQGDLIANSQYPLTQYYPNSGWVE
                     HDPEEIWQKTLNAMRDVVSQVQGKAILCCGLTNQRETTLIWNKKTGECLAPAIVWQDR
                     RTEEFCYSLSEYSSMIQEKTGLLPDPYFSASKLNWLLKNNAQAQSLINQGDLAFGTID
                     SFLIWRMTKGASHATDITNASRTMLFNIHDEKWDKQLLELFSISESVLPAVYSSDNHF
                     GVIDKEWLGVEIPITGVAGDQQAALIGQGCFNGGMVKATFGTGGFLLLNTGDKPVFSK
                     HKLLTTIAYKIKEKTVYGLEGSIYHAGTTVKWLRDEMKLISHSAETEVLARSLDSNNG
                     VYLVSSFTGLGAPHWLSITGTAIIGVSRSTNRAHFARAALEGVCYQTRDVLTCMRDDS
                     QLDLTLLRADGGMAVNQWFLQFMADQCQLTIQKPKDIETTAKGAAVLAAIGSGLFDSV
                     SSVNKIWDVENEFQPFREREVVEKDFRGWLEALSMVKARHA"
     gene            complement(57239..58510)
                     /gene="gltA"
                     /locus_tag="I9760_000059"
     CDS             complement(57239..58510)
                     /gene="gltA"
                     /locus_tag="I9760_000059"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAT5041862.1"
                     /translation="MTQKTAKLSIDGQETIDLPIYTPTLGNDVIDITQLGSHGVFTFD
                     QGFLSTASCESKITYIDGDKGILLYRGYPIEQLAEKKDFLDVSYLLLNGELPNKEEKK
                     AFVSLINNHTMVHQQMYQFLNGFRRDAHPMAIMVGIVGALSAFYHESMDLNNAQDRFI
                     SAIRLVAKMPTLAAMSYKYSIGMPYMYPKNKMSYAENFLHMMFGVPSEDTTPDPVLVR
                     AMDTIFILHADHEQNASTSTVRLAGSTGANPFACISAGIGALWGPAHGGANEACLNML
                     KEIGDVSRIDHYIKRAKDKNDPFRLMGFGHRVYKSYDPRAKVMRKTCYDVLEAVGAKD
                     APLFKLAMELERIALEDEYFIEKKLYPNVDFYSGLTLSAIGIPTNMFTVIFALARTVG
                     WMSHWMEMVATKSRIGRPRQLYTGEKIRDVK"
     gene            58736..59617
                     /locus_tag="I9760_000060"
     CDS             58736..59617
                     /locus_tag="I9760_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HAT5041863.1"
                     /translation="MITPAELPINHRGAVYHLDLRTEEIADLIITVGDPGRVSLVSQH
                     FDKIEVQRSHREFVTHTGYLGTNRITVLSTGIGMPNIDIVMNELDALANIDLSTRTVN
                     TTKKSLTIIRLGTTGALTENCLPGDIAISRYAIGFDSLIDYYQYQPLGVLETIKEELS
                     QYLKGESGPFYISESCSQLAEQFSSLGHTGITATCGGFYGPQGRQLRLPLHYPNLIRQ
                     LGTFCTQNYSVINLEMETAAIYALGSMLGHKCLSLSVVINNRIHGSFAADVKSCLDSL
                     ITKSLANIKNILELSEA"
     gene            59701..62292
                     /gene="gyrA"
                     /locus_tag="I9760_000061"
     CDS             59701..62292
                     /gene="gyrA"
                     /locus_tag="I9760_000061"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAT5041864.1"
                     /translation="MVYLAKEVLPVNIEDELKQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMSELSNDWNKPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSMRYML
                     IDGQGNFGSVDGDAPAAMRYTEVRMSKVAHALLADLDKETVDFSPNYDETEFAPVVLP
                     SRIPNLLVNGSSGIAVGMATNIPPHNLTEVINACIALVDEPDTSLEDLMEIIPGPDFP
                     TAAIINGRAGIIEGYRTGKGRVVIRARTEIETDESSGRQSIIIQELPYQVNKARLIER
                     IAELVRDKKIEGISGLRDESDKQGMRVVIELKRNEVADVVLNNLFAHTQMQNVFGINM
                     VALVDGQPRTLNLKQILEYFIKHRREVVTRRTIFELKKARSRAHLLEGLGIALANIDE
                     MIALIKQSPTPQDAKSALLSKIWQPGLVKAMLEKAGSNASRPDDLTEEYGLHESGYKL
                     SEAQAQAILELRLHRLTALEQDKIINEFEELLNLIKELLDILASPERLMQVIRDELIE
                     IKSQFGDERRTEITASQEDLTIEDLITEEDVVVTLSHQGYVKYQPITAYQAQRRGGKG
                     KSATHVKDEDFVERLVIASTHDTLLCFSNHGKLYWLKAYQLPQASRASRGRPIINILP
                     LAEGEEINAMLPVREYKDGSYVFMATKKGTVKKVPLNAFSRPRSNGIIAVDLEEDDSL
                     VGVDITDGARDIMLFTDAGKVIRFDESKVRPMGRTARGVRGIRVEKDQAVKSLVVVDP
                     SGGTILTATENGYGKRTHIDEYRVSGRGGQGVISIQVTERNGKVVRSLQVTDNDEAML
                     ITDKGTLVRFKVNELSVIGRNTQGVRLINVSSGEAVVGMQKIVDLGEELEEAEDSSLN
                     AEDSSDE"
     gene            62285..63373
                     /gene="serC"
                     /locus_tag="I9760_000062"
     CDS             62285..63373
                     /gene="serC"
                     /locus_tag="I9760_000062"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAT5041865.1"
                     /translation="MNSRVFNFGAGPAMLPEEILKEAQEEFLNWRNTGMSILEIGHRT
                     PEIISLLSTAEQSLRELLNIPKNYHVLFLGGAARAQFAMIPMNLLRPGDDAAYFITGI
                     WSKMAYHEANLLKKAYYLSSEEKEGFVSIPDYQKWELKSNTAYVYYTPNETINGVRFP
                     YVPKTEGVPLVADMTSCLLSEPINIRQYGLIFAGAQKNIANAGLTVVIIHEELLHNQP
                     EPVIPTMLNYKNHADHRSLYATPPVFNCYLASKMFEWIKKQGGIEELFQRNCLKAAKL
                     YQYLDSTDFYLTPVSKEARSIMNVCFSLYYPDLEQKFLDMANERGLKALKGHRFTGGL
                     RASLYNAMPMAGVDALIEFMSEFAKENG"
     gene            63379..64680
                     /gene="aroA"
                     /locus_tag="I9760_000063"
     CDS             63379..64680
                     /gene="aroA"
                     /locus_tag="I9760_000063"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAT5041866.1"
                     /translation="MLNFISKPVGCLKGEITVPGDKSISHRSIIFGAIAIGTSVIDGF
                     LDGEDCIATLKAFQSMGVRIEGPDKQRVIIHGVGKYGLKQPQNIIDCGNSGTSMRLLA
                     GLLAAQQFDSQLTGDESLLKRPMLRISRPLSQMGADVTTQDGKPPIVIKGGKKLNGIH
                     YVMPEASAQVKSCLLLAGMYAEGQTKITENAVSRDHTERMLRTFSYPVQIQDGAIIID
                     CNGECHGTRLNIPGDISSAAFFIVAASITPGSDVLIRNVGINPTRTGIIHILTEMGAD
                     IRVLNQRAYGEEPVADLHIRYSQLKGIDIPASMVPLAIDEFPVIFIAAACAQGKTTLH
                     GAKELRLKESDRIGAMVDGLNQLGVHAEGFDDGILIEGGSIQGGEVNSRGDHRIAMSF
                     AIAGAVASAPVTIKNCANVATSFPSFVTTANMLHFQIEEYS"
     gene            64680..65372
                     /gene="cmk"
                     /locus_tag="I9760_000064"
     CDS             64680..65372
                     /gene="cmk"
                     /locus_tag="I9760_000064"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAT5041867.1"
                     /translation="MVFNNEVPVITLDGPSGTGKGTICHLVAKRLHWNMLDSGAIYRV
                     LAYAARKNSIEPNDIKKLTALARSLNLRFESSADNETKVILDDENVSQQIRSEQCGQD
                     ASQIAVIPEVRAALLERQRNFAQFPGLVTDGRDMGTVVFPNAVLKVYLYASAEERANR
                     RYLQLQDNGINVSLAQVVEELAKRDARDTARTHAPLKPAEDAVLIDTTGLTIVQVFNI
                     VLKLIDERLNNL"
     gene            65438..67114
                     /gene="rpsA"
                     /locus_tag="I9760_000065"
     CDS             65438..67114
                     /gene="rpsA"
                     /locus_tag="I9760_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAT5041868.1"
                     /translation="MSESFKELFEQSIAGAQFYPGAIITAKVIDIDDDFVTLNAGLKS
                     EGIVAVEEFYDKNGELEVKVGDTVEVALDSVEDGHGETLLSREKAKRQEAWRKLSKCH
                     ENNETVTGLISGKVKGGFTVEIGSIRAFLPGSLVDVRPVRDPSYLEGKELEFKVIKMD
                     LKRNNIVVSRRAVVEEESSADRQALLESLHDGQELHGIVKNLTDYGAFIDLGGIDGLL
                     HITDISWKRVKHPSEVLSVGQDVKVKVLSFDSERNRVSLGMKQLGNDPWVDLVERYPI
                     GKRLQGKVTNITDYGCFVEIEEGVEGLVHMSEMDWTNKNVHPSKVVSLGDVVDVMVLE
                     IDEERRRISLGMKQCVGNPWQQFASTHNKGEKVKGKIRSITDFGIFIGLDGDIDGLVH
                     LSDISWTVPGEEAVKQFKKGQELEAVILAIDPERERISLGLKQLEGDSFASFAETYTK
                     GSIVKGTVTAVEPKTVTVALAEDVSGTIRVSELSDERVDDATTIVKVGDEVEAKITNI
                     DRKNRTISLSVKAKDAQDEADAIKKYSRTEAASTTLGDLLKEKMASKEGE"
     gene            67227..67517
                     /locus_tag="I9760_000066"
     CDS             67227..67517
                     /locus_tag="I9760_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HAT5041869.1"
                     /translation="MRILMLVTYLLLIIIGVSFAALNASSVQVNFYFKTVSMPISVLM
                     TIMLGVGVFIGFVLFITRYWRLKIECRKMKNQLKLTEKEIKNLRSIPLQDQH"
     gene            67564..68733
                     /gene="lapB"
                     /locus_tag="I9760_000067"
     CDS             67564..68733
                     /gene="lapB"
                     /locus_tag="I9760_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAT5041870.1"
                     /translation="MINLWPLLLPAAAWSGWWLANRSNSNKDAHVHNRLSREYVVGLN
                     YLLNEQSDKAVDVFIKLLEVDSDTVETHLALGSLFRRRGEVDRAIRIHQNLIARPQLS
                     IQQRKEALMALGQDYMSAGVFDRAERIFLEVVELGGSRDTSSLQGLLAIYQQEKAWEK
                     ALDIIKKLEISTGASFHNQAAHYYCEMASQALKINAMDRAIYCIKQAMNVDPESVRAS
                     LMNATIEIKEGRYKQAIRSLKRVPQQDAEFLTEIIEPLVLCHKELDSMPDCIHYLEQT
                     LEKHPRASVIFVIADYLRQNKGMDIAIDFVADNLSKHPSIRGLNRLIYWHLESAHGKV
                     REKLQMLYDITSKFLDNKPIYRCGHCGFGGKHLHWHCPSCKQWGRMKPIHGLEGD"
     gene            68997..70112
                     /locus_tag="I9760_000068"
     CDS             68997..70112
                     /locus_tag="I9760_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HAT5041871.1"
                     /translation="MQFIDLKTQYSLIENDILNSIKRVLNHGQYIMGPEIAELEKELA
                     TFVGVKHAIVNSSGTDALLMALMALDIQPGDEVITSPFSFFATAEVISLCHATPVFVD
                     IDPLTYNLDPEQLERAITKKTKAIMPVDLYGQCADYDVINAIATQYGIPVIEDAAQSF
                     GATYKGKYSCSLATIGCTSFFPSKPLGGYGDSGACFTNDDVLAQKLIEIRIHGQNARY
                     CHHRVGINGRMDTIQAAILLQKLKIFSNEILLRQKVAKRYDQMLSELVKTPYVHECNA
                     SVYAQYTIEVENRDVFAKSMQASGIPTAVHYPIAMHQQQALGYLNYKLGDFPNSEKAS
                     QHVISLPMHPYLQEDEQLKIVAAVRNALLVMNEEAMV"
     gene            70113..70802
                     /gene="pyrF"
                     /locus_tag="I9760_000069"
     CDS             70113..70802
                     /gene="pyrF"
                     /locus_tag="I9760_000069"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAT5041872.1"
                     /translation="MTPKLIVALDFDNQDNAIQLVEKLDPNHCALKVGSELFTLLGPQ
                     FVKALVRREFKVFLDLKFHDIPNTVAKACHSAAELGVWMINVHAIGGLKMLQAARESL
                     KTYGKDRPLLIAVTVLTSFEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHE
                     VKIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNP
                     YEVVSALLRDL"
     gene            70989..>72551
                     /gene="vpdC"
                     /locus_tag="I9760_000070"
     CDS             70989..>72551
                     /gene="vpdC"
                     /locus_tag="I9760_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VpdC"
                     /protein_id="HAT5041873.1"
                     /translation="MMTPQHVISQLLESDTKYPQNLDLLKKIIISAYLGRLRINGLPP
                     DNKIALGNYLFDDERMIFDFTRLSDSKRALFIEWLLEPHQKDKEHGFLSGVYVNEYRG
                     FTAEVALSWWGILKNWLFNNKSEHWKITDLGLSVNYQLTGIEMCHGKQGILIGFNQFL
                     VPPSGTKYRDPNDSQSEPLGNVKRVFITDKLVDELVGLNYKKLNYESVCKSPHPQSID
                     VTDPEARHKDMYDYRKMQRFNGVRPWYIRLWRWISKLFFGDTYDAKKTSTSDDNLELL
                     YKTKTVKIYQRSKSKEILVTEKRPDITNLVFCGGGAKIFAHVGVWKALNEAGMKPTKF
                     AGSSAGAIMSLMCYLGYTADEISELFKHFKQEHLVQFDIDRNGLSDTHSLKTALDYAI
                     ANKVRQIVNKYKIPYPKGKITFSTLASLREQFPDCGIGKELIVTATNKRSGKTIYLSA
                     NRYPLLEVSEAVKISASFPVLYRDTLLDGEEHNDGGILSNFPTEVFFDDHSTLLESEF
                     GNNLKVLAVQFDN"
BASE COUNT        22695 a        12501 c        15038 g        22317 t
ORIGIN      
        1 ataatcccca aataaataat gccgcaaaac cccaacaata aataaacttg tccttttgaa
       61 aataaccata tagtgaaagg aacaaaagca gtgaccagaa ataaaaaata ggataatcgc
      121 acaggtgaat agagcgaatt ggctgctgct gtaactcctg taataagaaa aacgacaaag
      181 atttgatgca atgtacttga ggctggaatc ataattgaac tggcaaatcc ccatgccaag
      241 ccagaactta caacacccaa gttaaaaagg aacaaccatt tttcggttga aaataatgtt
      301 ggttgtttat tgtaatagaa gagcagcatg cttttataaa gatctgaaaa aacataagta
      361 aaaatcatcc aaataataag cagctcatga tttgtaacag accataatgc aataggtagt
      421 atagttactg ttaaagcttc acctgatata gccataccaa cttgatcaaa aagcagtttg
      481 acctgctcct tcttaatttt tgatttcaac tcagtggaag cagtatggtt tttcttgttt
      541 ctgtttttaa taatattaat cataacaagt agttagtaaa agggtatgtt aaaaagcgaa
      601 gcgagcatta atgttgatgg ttttcctcgt aaacagttag aaaataaaac taagtgcatc
      661 gtgaaatcca aatgaatttg atgatatcta ttagtatagt aaacaatcag attgtattaa
      721 aatatatcga ttagtggggg atgaaatctc agcaagaatt gcattttcca gaaactaaaa
      781 gcactcattc ctggtaaaaa taaaaatcgg atgacaaccc attaactaaa agttggattc
      841 tggcgtattc atattgtttg gaacatttga gcttaagtca aaaattatta atactctctc
      901 taatgacgct tgttaataac ttaccctttc tgtcacgact acgttttaat aaagaataca
      961 caactaaact aaattcatca ttctttgcat aggcaacgct gagataatat tgactttcaa
     1021 cggttatttg gtcactggga atattgaatt tttttaataa ctcgttgatc tctttcagat
     1081 cagtgatacc attttcccca cgagcagtta ttaattcatt tgcctgggcg tcacttaatc
     1141 catctcctaa tgacatcaat atttgctttg gggcagtatt aatgttgatg ctcgttgatt
     1201 caggcaaggc tgtgataaaa ggttcaaggg caatataagc gggtgcactt acatctttta
     1261 tcaatcgaaa ttcagattta ctttttataa gttgatggct gggatagtaa ggaggtttct
     1321 gggagagata ataagaggta taaatatctt ctcctcggcc aagatcataa gcaagcaacc
     1381 aatgctttac tcccaaggct atattagcac gttcttggcc ggttgccttt gagtaggcgt
     1441 ggcttatcag gtgcattaaa gtgggaatag attttttctc aatcaggtta ttgaggttaa
     1501 atcgtgcttg taaatcaaaa attccaccac ttaattgaac ttgattatat atggattcca
     1561 tatttttagg gtattgggct accatcccta actggttggt ttttgtaaag tggttgttct
     1621 tatcaagtaa ttcattgagt gcccagaaag taactgcctg agaagcaaga tatagtttat
     1681 cctgagcaat cactagtctt gttctgtaaa tgtcctgttg taatcgtgtg ctcatggcag
     1741 tggcaactat agcaaccaat gtcataataa atagagccgt taaaagagcg cttccttgca
     1801 ttttagaact ggcaatggat tttgaaggtg ggctactcat aaacatagag tgctccgggt
     1861 atgataaaaa gcatgttcat atctccccag tcctttaaag tgagattcat ttggattgct
     1921 ttgggaagag gttctctttg ctgattttga ttgactgcct gttctcgcca ctcggagaga
     1981 agctgtaaat tttgatttaa atagttgaaa tgacattcag acaaattatc cagaagtact
     2041 ttgtctttat agatatttct gtcaatagga tctaaagtat tccatgtgcg gcgcagtaat
     2101 ttgctatcct ggcaaacaag tgcgacgcgt tccaaggtgc tgcgtttctc agtactgttg
     2161 gggtttacta ctccatccct ggttagttca aggtattggg gttgaccaac aaatattgga
     2221 aacaggcgca tttcattacc tcttatagca cgttccactg tttgggaagt atcttgttga
     2281 atgatactta ttgccagctg aagtgcattt aagcgttcag cctgcgcgtt aactcgggtt
     2341 cgagtattga atgcgtagta taaggttgag gaggtaatag ttgctaatat agcaaaaaca
     2401 gtaagagcaa ttaatatctc aatcagtgta aatcctttat ttttaatcat ggcaagtacc
     2461 tgaatgcaat gagttcttct cgaaagggac ctgcttgttt ggaactgact gaaatggtta
     2521 tctgttgcat tcgttttata ggggtcgggc taattttagc tctccagtac cacttttgtc
     2581 ccagcatggt ggttgcttgc gttgattctt ggctttgaga agtgcgcagc agatttaatt
     2641 ggatcatcgc tatcccttgc attgctaccc aatgactaat tgttttttct ttaattctat
     2701 gcgtgttgtc tatattttga gcagttgctt ttaaaagcgc tgttaaagca attgcaatga
     2761 cagtaagagc caataagact tctatcaaag taaatccgga gactagttta gttttcggca
     2821 tcattgagat tttgccatgg taaaatccaa atcaccatta tggctgcctg taagtttggc
     2881 tatccatttt tccttatctg tgccaaattc tatagtaaaa ggggtcatgt caccagatgg
     2941 tgctattatg attgcagggt tcccccggct atgttttaga tttgttttaa ggctaatcac
     3001 aacgtttttg ggaaaatagt agagtttaaa aattcctttg ttcgatatgg gattccatcc
     3061 cgcctggtcc tgaaacttaa gaatttggta actgctatta tctattttta acccaagggt
     3121 actgctttct aaaatggctt gttgttgagc caggcttagg gtatttgcca attgttcagc
     3181 tgaaaattgt atacgtctgc tttctccgaa atcaccaaaa gcgattaagg caaaccccaa
     3241 ggttatacct ataatcacta tgacaataag aatttctatc agggtaaaac ccttatgagt
     3301 ggtcatccca gttaccaatc tccgcattaa tccctgttcc gccaggttgt cctgctgctc
     3361 cataagtaaa gacatcaacg tcagtatgtt gtcctgggtt taaataaaga tattctctgc
     3421 cccatggatc ttttggcaat gatttcagat attgtttcca attcgttgga acaggattgc
     3481 ttgttggttt ttctaccaga gccattaaac cttgatcagt tgtagggtat gaaccattat
     3541 caagtttgta taaatcgagt gcattttgaa tggctaaaac atcttgtttc gctttgactt
     3601 tacgggcttc atccggacgc cccatgatct tggggacaac tatagatgcc aatataccta
     3661 aaatcacgac aacaaccata atttcgatca aagaaaagcc tttttgtcga ttcatttgtg
     3721 tacctcttgt aactaaatgt taaattataa cacaaatatt gatgcctatg aatagactta
     3781 aatttttaaa gggcgcggat aacccttcct ctgcttaatc aggcaactaa ttgttccatt
     3841 gaaaaaatag gtaataaggt cgcaagtaca ataaacaaaa caacagcacc cattaataga
     3901 ataaccatag gttctaataa agttaaggat gtttcaatca gtcggtttac ttcattatct
     3961 aaatgagacg ctgagcgttc catcatgtct gaaagttggc cgcttttctc accactggct
     4021 atgagatgga tagccatagg gctaatgaat tttgtttctt tgagggcttc actgattcca
     4081 cttccttcac ggactcgtag agtagcgaaa tcaaaagcct gtctcatcac gatattggtt
     4141 accaaacttg aagaaacacg catggtttca agtacactga ctcctgctgc aaatagaatt
     4201 ccaaacgtgt gaatatatcg tgcgacatta attgttttaa ccaaataaga cacgataggt
     4261 agtttaagca tgaattgatg ccatgctgtt ttaattttta tattactgag gcttttttta
     4321 aatccaatca gagccacaat tattgtcact agactataca aaccataaga tttgataaat
     4381 tggctcaagt taataagcaa tttcgtcatt ggtggtagtg tttggccact ttcggtaaac
     4441 acatcaataa ttttagggac gacaaaagta agtaaaaaac tgataatggc agtagaaaca
     4501 atgatcatga gcaatgggta aatcaaggct tgttgcactt tctgtcgagt ttgttgttgt
     4561 ttttcagtat aatcagctaa tttttctaaa acgacatcaa gacgccctgt ctgctcgcct
     4621 gagccaactg ttgcccggta tagttctgga aaggcattgg gatattgagc cattgcctga
     4681 gcaagaccat acccttccaa tactttagag cgaactccga taataagctc tctgactttg
     4741 tctttttctg tttgttcgct gaccccgcgc aatgattctt caacaggaat accggcagcc
     4801 agaagtgttg ccagctgtct cgtgagtaat gccaaatcag ccgcagaaac tttacttttg
     4861 ctgctggcag cacgttgctg ggttaatgtt tggacttgag tagggattag tccttgttct
     4921 cgcaataatt gacgtgcatg gcgttcagaa tcagcttcta ttacaccttt gctaatgctg
     4981 ccattcactt ttagcgcttg atattggtag gcgcccatat tatttttgtt aaaattaaaa
     5041 ctatagagtg taatctattg ggattaataa gtcacttaag gtaaactata cgcggactat
     5101 cctggagaca aaagatgaca aaaaatgtag tgcttgatgc aatcaaagaa catgacgcca
     5161 aatttgtgga tttaagattt acagatatcc gtggaaaaga gcaacacatt accatacctg
     5221 tttcagcagt tgatgatgat tttcttgaga atggaaaaat gatcgatggc tcatctttta
     5281 aaggttggca gaaaattcat caatcagatt tggctttagt gccagattta acttcaatta
     5341 agccggatcc attttttcag gacaacactt tatttatacg atgtaatgta gttgatccac
     5401 agactatgat gggttatgac agatgcccac gttcaatcgc acaacgcgca gaaaattatt
     5461 tacaatctac agggattgca gatggtgtaa attttggccc tgagcctgag ttttttctct
     5521 tcgacaatgt gcaatgggaa actactatca gcggttcatt ttataaaata gattcagacg
     5581 aggcacattg gaactccggt aaggaaatgg aaggcggaaa tattggccat agacctccgt
     5641 tgaaaggcgg ttatttccct gttcctccag tcgattcatc tcatgatatt cgttcagcaa
     5701 tttgtatcac attggaatct ttaggaatgg ttgtggaagc acatcatcat gaagtagcta
     5761 cagctaacca gtgtgaagta gctacccgtt ttaatacact caccaaaaag gcagacgaac
     5821 tacaaattct gaaatacgtg atacataatg ttgcccataa ctatgggaaa accgctactt
     5881 ttatgccaaa acctttggta ggagataatg gaagcgggat gcactgtcat caatcacttt
     5941 ataaagatgg gtataatctg ttttccggtg atcagtattc ggggctttct gaaacagctt
     6001 tatattacat aggtggaata atcaagcatg ccagagcttt aaacgctttt accaatccgt
     6061 caaccaatag ctataagaga ttagttcccg ggtttgaagc gcctgtatta ctggcttact
     6121 cagcaagaaa tcgctccgcc gctatccgta tcccacacgt tagcaatcca aaggcacgac
     6181 gtatagaagt acgtttccct gatccaacag ctaatcctta tcttgctttc tcagcaatga
     6241 tgatggcagg catagatggc attcagaaaa aaatctaccc tgggcagcca atggataaag
     6301 atctttatga tttaccgcct gaagagcttg ttgatgtacc cactgtttgc tcttcattag
     6361 agatggctat ggaccatttg cgggcagatc atgaattttt gttacaggga gatgtgttca
     6421 gcagagactt tattaataat tatattcaaa tgaaagaaga ggaaattact aaaatcagga
     6481 gcttgactca tccttatgaa tttgagttat attatagtct ctaagggtct atattcatga
     6541 ataaactgcc actattcgtc tttttgatga tggtaatttg cctttccagc gcgcaaattt
     6601 acaaatggac ggatagtcag ggtgttgttc attttagcga caaacctcat cagggtgctg
     6661 agaaaataaa aattcctgaa gctcaaactt atacaccacc tgcaaatgaa acaggtggtt
     6721 cttctgagca agaacaaaag agtgtcaaac aggaagatca ggaaaaatat accaaattag
     6781 aaattattca accgcaaaat gaagcaacaa ttcgtaataa tcaaggttat gttgttgttg
     6841 cagtccaaat agaacctgat ttgtctccag gggataatgt gcagcttatt tttgatgggg
     6901 cgcctttagg agagccacag cccaatttga tttttcaatt gaatggtatc tatcgaggat
     6961 ctcatacaat tgctgttcag gttcttaatg ataaagggga tgtgcttaaa gtcagcgaca
     7021 gtattacaat tttcatgcac aatcctcgcg tcggcatggg caagggcaga aagaaaaata
     7081 tcaattctaa taaatagagc aggttatgta tgttaacttt gttatcgcca gctaagaaac
     7141 tattatctat atcaaaacat tactctaaag aaaccagtaa tcctttattg ctcgataaag
     7201 cgctacaatt ggttaaaata atgaagttaa aatctgtgga acaaattgct gatttaatgg
     7261 atttatcaaa acaactcgct gaattaaact atgagagata tcaaaatttt gatcttaaaa
     7321 acaacccaat gaatcactct tatcccgctt tgtttttatt tcagggagat gtgtaccaag
     7381 gattgaatgc taattcatgg aaagatgaag agatcgaata tgctcaatct catttaggaa
     7441 ttttgtcagg gctatatggg tttttaaggc ctttagacag gattcaacct tacagattag
     7501 agatgggagt taatttagaa aatccggcag gtaagaatct ctatgcattt tggagcaaaa
     7561 ttgttactaa tattttaaac caaatactgg cagagcagag taatcctgtg cttattaacc
     7621 tggcttcgac tgagtatttt aaagtcgtgg atgagaaaaa attgagttat cctcttgtga
     7681 ctatcaattt ttatgaacag aaaaatagtg aattaaaaat gataggtatc cttgctaaaa
     7741 aagcaagagg aatgatggca aagtatataa tgcagaatag aatagactcc atagaacaaa
     7801 ttaaagagtt ttctgagtcg ggttatttat ttaataaaga aatttccagt ccaaatagtt
     7861 tgaattttat tcgaatccat taaattattt aaaaggtatt tgatgtggaa tttgtccagt
     7921 cgggtgcatg ccctgaatca tttccccaag caaggtatag agtgttatgt taagagagat
     7981 gatgaattag gttgcggcat aagtggtaca aagataagga aatacagcag tttaatcccg
     8041 tttctaaaaa tcaagggaat tcgacatctc attattattg cgggtgctca atctaataat
     8101 ttattggctg ctctgcaggt ggccagagaa tgtcaattga aagtgacagc cttcctagta
     8161 aaacccaaac atttaaaaat ccagggtaat tttaaattaa gccttctttt tcttcatgag
     8221 aatgaaatta tctggataaa tcgagaagaa tggtatcgag tcaatgaatt cgctgagcag
     8281 tatcttgagg ggctcagaga aactgcttat attttatctg aaggtgccag tgttaaggag
     8341 tccatgaagg gggcaatgtc tctagccagt gatatcaagg aaaatgaaaa gattttggga
     8401 tttgcttttg atcatatctt tgttgatgca ggtacaggtt tttcagcaat cgcgctaatt
     8461 aaaggttttt atgaattaca gcataaagga tttattcatg tgttattgtt agcagatagt
     8521 gaggaggttt ttaagaaaaa acttatgcat tgggcaggag taaaccctga taattatgct
     8581 tgcttttacc caactactgc gaaatcgttt ggatctgtaa atcaaacaat taaacaagaa
     8641 attaaacgat tggcttatga agaaggcatt ttagctgatc cgatttactc tgcaaaatta
     8701 ttttatgagg ccagaaggta catagaaaaa tacgaattaa aagggaaggt attgattgta
     8761 cattctggtg gtatattaac catgcctggc tttgcttttt gaataggcct gcttcttggc
     8821 aaagacgtaa tttctgaatt atcacgtttt cctgactgca aaattaagtt catcaaggat
     8881 attcagtata tttcagtaca taatgcgatt tttttcctga atatccctgt ctcgattatc
     8941 tactatactt attaattata aaaataaaaa ccgttctttt taagaaaagt tatcttatat
     9001 gggagacgag tatcatgaaa gcaagaaggg atcaacaact ctctaaattg cgaatgagat
     9061 ttttttcagc gctcaatcac acatctgaaa ttgacttaca agtgcttttt aatgatttga
     9121 aatcaattct cacactggat agtattgagc atctcaaaga aggctctgta gcgtatgcaa
     9181 tcattcaaga actcttgaaa caggatgatg ctcaaaataa gattcaatct tttttacatg
     9241 gagcgatcaa gaatgttatt catccgggtg tcatcaaagg gctgacccct gatgaaatta
     9301 actggaatgt tgccaaagca tacccgaaat attatgagca tgaggaattt cctgatgtga
     9361 cttttggcgg ctttaaagta cgtgatagta atgaatttaa atttaagacg aatatacaaa
     9421 cttcgatatg gtttagtatc aagcccgatc ttttcatgcc ttctaaacaa caggaggctt
     9481 taaaaagaag aagggaacaa taccctggtt gcgaaattag attaatttat tccagttcat
     9541 tgctaaatgc agaagcaaac cgccagatga aggcatttgc caggaaacaa aatatttcat
     9601 taattgatat cgatagtgtt aaaaccgatt caccattata tccattacta aaagctgaac
     9661 ttgcacatct tggaaaagga ggaaatcctg ctgctgcctc cgatctgtgt cgctggatcc
     9721 ctgagttatt caatgaaggg ttttatgtgg atattgatct ccctgttgat agttcaaaaa
     9781 tagtagaagg acatcaaatt acaggaggag ttccaatcat gctcaatatg ggctccatta
     9841 tcagtgaacc catagctcca catcatagaa ggcaagaagc tgtttgtatg aatacggaca
     9901 ttattgctta ttccaatgat aaaagaactc aaaaaatgat ggatacggtc gcccgatatt
     9961 tgaaaaatat atacgatgat ccatatacag cattaaaaga cacacctctt gcgcaaacgg
    10021 ctttttttaa taagtgccaa gaggaaagga aaagtatatt tgatttaaga aaaggattac
    10081 aagatgcatt tcgctccgat tcattattgc aattatacga ctttttaggg gcagacaaat
    10141 ttaaggaagt atttaaatta aaggaagctc aaagcaaata cattaatgag catataagtg
    10201 aatttagcga gaaggatctg ttgttaaatt tgatttctga taaaccttcc gagattagtc
    10261 agcatacact tgattttgtc aaggctaaag caatgtacat agatattgcc aaagagcact
    10321 attctgcatt ctacaaacca ttagtcgagg agatttcagg gccaggtgcc atttataatg
    10381 ctttaggagg agcgggcagc ttcactacca ctcataggcg gttaacagga cctatgttgc
    10441 caacaacccc accacgtgtt ttacaggtat tttgtgatgc acatgacaag ggtccttttg
    10501 tttcagataa tattgcgcgt tggcaaacca atgttcgaga tctgggcgtg ttgaatcgag
    10561 agggtttgag ttggttgcct tcagtagggt agcttaattt caacacacaa attttgaata
    10621 ttgtctcaag cattagcaat atgaataatc accaatagat aaaagacttt attggtgatt
    10681 agtttttttg ctggtttcct cttttttagc gctatcccct tgaaatttaa tagatcaacc
    10741 ctatatgaga agttaattat tcgaacacaa ctattaacgg ggagattgtt taatggttag
    10801 caagcagcaa actatgggat ttcaaacaga ggtaaagcaa atgcttcatt tggttgtgca
    10861 ttcgctttat agcaacaaag agatttttct tagagaatta atttctaatg cttctgatgc
    10921 cttggacaag ttacgatttt tagctttatc aaacgggtca ttgtttgaaa atgattctga
    10981 tttgaaaatt agcattcaga ttaatgaaaa gcttcaaacc attaccatca gcgataatgg
    11041 gattggtctg agttgggaag aggcagtaga aaatttgggg acaattgcaa aatccggaac
    11101 aaaagaattt attagccaat taactggtga acaagcaaag gattctcaac taataggaca
    11161 atttggagtg ggtttttatt ccgcgtttat cgttgctgat aaagtaacag ttaaaagtcg
    11221 ccgcgctggg ttacaacctg aggatggaat tgtatgggag tctaagggtg atggtgaatt
    11281 tactatagga tatgaaaaaa aatcgacccg cggtacagaa attaccttgc acttaaaacc
    11341 tgaaaacgac gagtttttaa gtgattggcg tattcgcggt attattagta aatactctga
    11401 tcatatttgt tggcctattg tgatgaaaaa gctttcagag gaaggcaaag aatcaaaaga
    11461 atttgaaaca gtaaacaagg cgacggcact ttggacacta cagaaatctg aaattagcga
    11521 agaagactat aagcaactgt ataaacatat ttctcatgat tacatggatc cattaacctg
    11581 gtctcataac catgttgaag gaaaacacga gtatattact ttgctttaca taccagccca
    11641 tgccccattt gatttatggc agcatgaagc caaacatgga ctaaagcttt atgtaaagcg
    11701 tgtatttatt atggatgaag cgacacaatt tttaccccga tatttacgat ttgtaaaagg
    11761 gattgtggat gccagtgatt taccgcttaa tatttctcga gaaatattac aggataacaa
    11821 gcaggtagaa tctattcgag ctgcatgcac caaacgtgtt ttgtctatgc ttgaaaaaat
    11881 ggctaccaat gataaagaaa cctatcagaa gttttggaat gaatttggac ttgtgctaaa
    11941 agaaggacct attgaggatt tcgctaataa ggaagcgatt gccaagttgc ttcgtttttc
    12001 tactacagca agcggtagtg agaaacaaga agtatcgctg gaggaatatg ttagtcgcat
    12061 gaaagaaggg caggataaaa tttattatat taccgcatcg agttacaatg ctgctaaaaa
    12121 tagccctcat ttggaaatat tcaggaaaaa gggaattgag gtattattgc tcagtgataa
    12181 agtcgatgaa tggcttgtag gttatatgaa tgagtttgca ggcaaaaaac tacagtcaat
    12241 ttcaaaaggc aaggttgaat tgggagatga tgaaacttcc gaacaaatta aagaacagga
    12301 aaaaaccctg gagccattga ttaagcatat taaatcagtt ctaaatgagc gagttaaaga
    12361 tgtattgctg actaatcgtc ttactgattc ccctgcatgt gttgttgctg atgagcaaga
    12421 catgggattg gaaatgcaaa gaattttaca agctgcgggt caacaggttc cagtgagcaa
    12481 gcctattttt gaaattaatc cggatcatgc cctgattaag cggttgcatg atattcagga
    12541 tgataaccaa tttgaattat gggttactat gctgtttgaa caagctgtgt tggcagaagg
    12601 agggcaattg gataatcccg ctgattttgt gaatcgggtt aacagacttc tggtttcctc
    12661 ctagaattct atagggttgg caatcaatcc cggattacaa caagtcatta tccgggatga
    12721 gctgaacata aaataaaaag gctcaaaaat gagccttttt atttttaaat ttcccttact
    12781 cttcagttaa gcgaaaatat ttggtaccga aatatctttg taatttcttt ctgatcgtac
    12841 cgcgactaat acccaacatc ttggctgctt gtaattgatt tccacgacgt ttttccatta
    12901 cagcttgtaa tagaggtggc tcaacttctg acaggatcat atcatagaga tcattaattg
    12961 atttggattt attttcagca agaaagcggg taaccaaact gtaaactaaa tcctgtaaac
    13021 cttgttcttg tttagtggat tgagttgtag cttgtgtatc aatgacattc atcttaactt
    13081 ccttattatt taattaaatc atacagtcca attgcttaca catgtaaatg aaagcaaaat
    13141 taattgtaca tgaaggctaa atgataatct agattttcat atcaaaacta tgcatttttt
    13201 tcaggcaatt ctgccactac aagatcaaag agagaacagt cagcctggct ggaagacaga
    13261 gattccttta actttgccag ttgattaatc atcgacttgg cttggttggg atcattatga
    13321 aaattggaaa tatatctaga taattcaatt gcattgcaat cgtattgcaa aaattcaggt
    13381 accatcattt tattggctaa cagattacat aagcccaaaa atttaacttt aataaaatac
    13441 atggcagcaa cataagtaag aaaggacgat ttgtaaataa tgcacattgg tttttccaat
    13501 aaggcacatt ctaatgaggc agttccagaa gctactatga caaagtcagc agcagacatg
    13561 cattctattg cctgtccctg gataaaagta acagtaagat tctggttgct gaaataagct
    13621 tttactttat caggattgat agttccagcg atagggacta caaatcgaag tttggggttg
    13681 tctagggtca gcaattttgc tgtattcacc aatatcggta tatggcgttc aatttcagaa
    13741 tggcgactcc caggcagtaa ggcaataatt ggctcattta aaggtaatcc caaggaagtt
    13801 ctgcccgagt gtttatcctt ggcagcagct atctttttta ctaaaggatg tcctacaaaa
    13861 ctgacaggaa cacctgcatt ttcatagata gtcttttcaa atgggaagat gacagccatt
    13921 ttatcgatgc aatctttaat aagatggata cgtttgcctt tccatgccca tatttgaggg
    13981 ctgatgtaat aaatgatttt gattcccagt tttttctttg catacttggc caatctcaaa
    14041 ttgaaagcag ggtaatctac caaaatcagc aaatcaggtt tttgtgtact cagatgctgt
    14101 ttgatatcct gaaaggcttt acggaatatt ttcaggaaag gaattatttc agtcaaaccg
    14161 gttacggcat aacgcgttaa atctgaaatt aaatgaaccc cagcggctct taagtgttta
    14221 ccgccaatgc cactaatttc caggtctgga taaactgctt ttaattgctt taccagctcg
    14281 gcagcatggt ggtcaccaga ttcttcgcct gccactatga caacacgact agcattcggc
    14341 atagcgttct acaaggttat tattaattaa agcggtgatt tgttcagctg ttttcaaagc
    14401 atcccttcct tcttcacctg taacaagcgg cacgctatca ttttctatgc aatttaagaa
    14461 agctctgatt tcctccagta acgcatctcc tttttcaaac tccctttgat ctctggtgat
    14521 atcaggtatt ccagggaaca tttctcccgt gcctttttta aatacggcaa gctgtttgtt
    14581 ttggtaatca attgaaatat aagaattctc ctgaaagatt cttgtttttc gttccgtttt
    14641 gaaactgatt ctgctggctg ttacatttgc tacgcagtgg ttagcaaagc ttatgcgtgc
    14701 attagctata tcaattgctt ttgttaatac cggtgctcct tgagccgtta tggatttaat
    14761 agggctctga actatatttt gaataatatc tatgtcgtga atcatcaaat caagtatcac
    14821 gtttacatca gttccacggg ggttaaaagg tgcgagtctc tgcgattcta taaataatgg
    14881 taattccaga tattcatcca gggcaaggcg agcagaattg aagcgttcta aatggcctac
    14941 ttgtaacttc acctgatgtt gttttgcaag tcgaatcagt tcctcagcct gggacactgt
    15001 ttcagtaatc ggtttttcaa tcagaacatg tatcccatgt tgtatacaga attttgcaat
    15061 ttcaaaatgc ttgttggtag tcgctgcgat actgacagcg tctactttgc caaacaggtc
    15121 atggtaattg atgtatgccg gaacatttaa ctcactggat accacttcgc agactgtaga
    15181 gtttacatca caaactgcaa ctaactctgc attaggaatt agttgatatt tttgggcatg
    15241 aaacctgcca agataaccca caccaataac tgcacaacga attttattca tctcatcatc
    15301 aattgataat tatttgtact tatgatcgca tttccttagt gataaatcaa ttgtatttcc
    15361 tatgttatca tgccagttaa atagtaattg aggcatttaa aactccaatc aaactcctga
    15421 gattggttca aatcaaagaa gtcgattctt gtcttgcact tgcattcaat agctattatc
    15481 ttacgcctaa tttttcgcag ggagcagtta aatgaatact gtgctaaaaa tattgatggc
    15541 tggggtcgat gaagtgggga gaggaccact tgcgggtgcc gttgtaaccg cagccgttat
    15601 tttaaaaaaa ccaatagatg ggcttaccga ctcaaaaaaa ttaagcccaa agcaaagaaa
    15661 tttattagca attagaataa aggaagaagc gttagctttt gcctatgggc gagcagaagt
    15721 tgaggaaata gatcaattaa atattcatca tgctactcta ttagctatgc gaagggcagt
    15781 tgaagcttta cccattcagc cagataatgt ctttgtcgat ggagctttta ctcctcaatt
    15841 gaacatacca tgcaaggcta ttgtccaagg agatagcttg atacctgaaa ttagtgcggc
    15901 atccatcctc gccaaggtat tgcgtgatga agaaatggta gctcttgata aaatatatcc
    15961 aggatatggt tttgcagaac ataaaggtta tgcaacccct gttcataaag aagcgctaat
    16021 gcgtttgggt ccttgtaaaa tacatagaag aagttactcc cctgttgcgg atttgatttc
    16081 aaagtaaaac tctgagtggt attaatttgg aaaataccgt tcaaataaaa atacataaga
    16141 aatagctttt aaaatgtcat aatatgattt atgcaaatca gagcataaaa aaataaatca
    16201 attcaagcta ttaaataata ttcggtgcga gcttatcgac attaaaattc caaagcttgc
    16261 caggaaagtt accatagctg ttccaccata actgacgagt ggaagaggta tacctacaac
    16321 cggaataatc cccatgacca taccaatatt gacaaatgca gaaaggaaaa aggtcatggc
    16381 cagactggct gaaagcaggc gcgtgtaagt ggtttgtgca ttattggcaa tatttaaact
    16441 gcgtagcgaa attaagacta tcagcgcgac aatagcaaat ccgccggcaa agccaaattc
    16501 ttcgccactt accgcaaaaa taaaatcagt agcatgttct ggcaagaaat tgaggtgtga
    16561 ttgactaccc tttaaccatc ctttgcccat taggccgcct gagccaatgg caattttgga
    16621 ttgaataatg tgatagccag caccaagtgg atcttgttca ggatcaatca gggtatacac
    16681 tctttgtttc tggtagtcat gcatcagatt ccacacaaca ggaattgctg agcacatcag
    16741 taatgcaatt aataatatga ttttaaaacg tattcctgcc aaaaatacga cacataaacc
    16801 tgcaacagta accataattg cagtacctag atcaggttgt tttgcaataa gaagagccgg
    16861 aataaaaata atcagggaag caataccaat tgagcgtatg ctgcttggat gagattgtcg
    16921 gtcgaaaaac caggcggcca tcataggtac agccagtttc ataatctcgg aaggttgaaa
    16981 acgaaataac cccagttcta accagcgctg agccccttta ccaattttac ccattagcat
    17041 gactgcgatt aacaagctta acccaacccc gtaaatccat ggggtccaga ttttatattt
    17101 atgtggcgga ataaatccaa gtacaaacat gattagaaaa gcgaagagca gcctcatgga
    17161 ttggcgcatg atcatgccca tattggcatt tgaagcgcta taaagtatca agagtccgaa
    17221 ggcaataaga gtaagtatta atccaagtaa tggaaaatca agatgaaggg actttgctgt
    17281 aaaacgatat acgggcttag tcgtttgtct actcatggtg ttttcttaat aggataaagt
    17341 tgataataag tgtcaagtac cttacgtgct acagtggaag cgatagtatc attttcaact
    17401 accaccgcaa tggcaatttc cggtttttct actggagtaa atgcaataaa taaagaatta
    17461 tccctcaaat gctcaggtat gtcttcgtac tttgcctttt catattgtct tccgctgtat
    17521 acttgtgctg tacctgtttt tcctgctacc gggtaaggag gatttctgcc aaaacggtaa
    17581 cctgttcctt cattgctggt tagtacgcta tgcatcgcat caatcacaac atcccagttt
    17641 gcttcatctc tcagggaaac tgggtattct tcaaaaggct tgtattcttc catttcattg
    17701 gtatcgctgt ttactgtttt tatcagtagg tgaggtctga atcgttgtcc atgctggctt
    17761 agggaagctg tggcattagc catttgcaaa ggggttgcca gcataaatcc ttgtcctatt
    17821 gcagaaatga gagtatctcc aggataccag gatactcctt tagtttgtct tttccagcga
    17881 gcgctaggta ttactccatt tgcttcttca tgaaggtcaa cgtgtgttag ttgtcccaag
    17941 ccaaatttta caagcatgtc ctcaatgtta gagattccca ttttattacc tagttggtaa
    18001 aaaaaagtgt cacaagagac ggtaatggct cgtttgaaat taatgacccc gtggcctgtt
    18061 tttttccaat cacggtaaac atggctggaa gtagggagtt tgtacttacc ggggtcatat
    18121 atttcagtgg tcgtggtaat gaagccttta tccaaaccag ccagtccaac aaacggtttg
    18181 attgttgatg caggggggta aacgcctcga actgctcgat taaaaagagg gcgctggagg
    18241 gcattggata acactttata atccgcttta ctgactccac tgacgaaaat attgggatca
    18301 aaacttggag aactcaccat cgcaagtact tcgccattcc gagagtttat gactactacc
    18361 gcacctcgtt tatccttcaa tgcattataa gctgcttcct gaagtctggc gtctatgctt
    18421 aaatacagtt tcgctcctga atgaggattg actttattta ttattcttaa agtgcggcca
    18481 ctaacatcag tttctaccat ttgataaccc actttaccat ggagaatatc ttcatagtat
    18541 ttttcaatac cggccttgcc aataaagttg gtagcacgat aattagtgga gtccacttgt
    18601 tttagttcct gaatattaat acgaccaaca taccccaagg cgtgggcagt aatctctcca
    18661 agagggtaat gacgcatcaa tctcgcttta atgcttactc ccgggaaatg atactgattg
    18721 ctggcaaagg tagctacttc ttcctggctt aattttaatt tgataggaat gggaacaaaa
    18781 gaacgatttt gtaatctggt cttattgaag ctatcaatat cttcgtctgt aattgaagga
    18841 attaactctc gaagtttagt caaagtctgt ttcatgtctt taacacgttc gggagtgatt
    18901 tccagcacat acactggtat gttttcagcg agtaatactc catttctatc caaaataacc
    18961 cctcttggag gagcaatagg tattacgctc atttgatttt ttaaagacaa ggtctgatac
    19021 cgtttaaatt cggatatttg caggaaggcc aatcttaaaa tcagtattaa agataaaata
    19081 atcagaatcg caattaacag attaagtcta aatcgttgat gttgggattc agcacgatag
    19141 tttttaaatg attgattccg atacatcgca gaaaatgttg tatgacaaaa caaaagtagg
    19201 aatagtacct gaaattacag atttttacca attataaaat atttataaag cttgtggttt
    19261 ttcatttttt gagattagag tcaaattttt ctatgtaata tagaaagagt gttgcaaaag
    19321 actatgcaca ccctatttat gataaggatg gttgttaatg atagaccatg ctctatagag
    19381 ggactccagc aaaataatgc gaaccagagg atgaggaagg gttaatttgg ataatgacca
    19441 gcgttcatcg caaagagtca gtatttcatt ggataatcct tctgggccgc caattataaa
    19501 acaaaggtga cttgaaattt gttgtaattg cgtcaaccta agtgctaatt cctcgctgct
    19561 aaaggatttt cccagcatgt ccaaggcaat aaggcgagca ttggcaggaa gggcatcttt
    19621 agtcaaagca ctttcttttt ctagtattct tgctaaatcc gaggatttat tacgacgcag
    19681 caaagggatt tcgatgagtt ttatctggat gccatcatga aacctctttg cgtattcgtt
    19741 aactcctgag ttaacccagt cgggcatttt attgccaaga gtgataatgg taatttttag
    19801 catagataat gtcaggagtt accagggtgt tcttcccata atccttcaag gttataaaaa
    19861 tgtcggaatt ctggttgcat gacatgcaat atgatgtctc caaaatcaat taaagcccaa
    19921 tcaccatgtt ctattccagt agaactcaag caaggcagac cagcaacctt catatcttcc
    19981 agaacttttt gagctatcga tttgacatgt ctggatgaac ggcctgatgt gacgaccata
    20041 aaatcagtga ttgttgtttg tttatgcaca tcaatgatct taatatctat cgcttgaatg
    20101 tcttcaagag attttaacag tttttcgagc gtaggatttt tttcaagcat aactaagata
    20161 tattccgtca aaagggttga ataatatcaa caattgattt gtataaaaag ggttttagtt
    20221 ttcaagtctg gaaaactaaa acgtaaaaat ctaattattg tttttcgcat atctgaatat
    20281 gagtcaataa aaatttaatt acagcaaaca ataccaggca gcctaacgta gcaatgaata
    20341 atatactgag ttgttgggtg aatgctgtag cgtagagcgt ttttaaaacg attatatttg
    20401 tctctccagt aggaatggca gtcagatcgg ctaattttcc tgaaaggaat cctcctattc
    20461 ctaatgaaac gaaaaagata cccatcatgg tgcttacctt gtttttgtca gcaagtacag
    20521 ttattgctga caaccctact ggtgaaagca gtaattcagc caaagaaatc atcaggtagg
    20581 cagggatgat aagaagaggt gaaatcaaaa tggatttatc aactacagta cttacaaagg
    20641 caataattga gtaggcgagt gtcatgaata ccatagcaag tacaaatttt ttcccggtac
    20701 ttaagcctct ttcaatcatt gtaagactac ggtgcttttt ggcaagaaaa taaccaatta
    20761 ttaacatacc aatactttgc actgtgatgt agtatggtgg gggaaaatcg atacctaaaa
    20821 agcttggctc gactactcgc gcaataaata atgttaagga catgaacatc tgaaaataaa
    20881 atgcccagaa aataacagag atgatgcaca ataacccgat aactaatgtt tgcctggact
    20941 gatttgcact ttcacgattt actgaatata aaatgtaact ggctgaaaaa agaaccacaa
    21001 gtccaaacat gatattcgcc agctgaggtg aacttaaaat ataaaatgac aatgaccaca
    21061 gcaaaaccaa taacacaaaa gctgaaatga ttttacctgg ttgtaaaaca aaaggattat
    21121 aatccttaat tttgtactga tgtattccaa ataaaaacac tacaaatgcg atgattagtc
    21181 cgattgatgc actggtgaag gaagcagacc agccaaaata ctcattaaac ttgcttggta
    21241 gtgttgttcc gaggattatt cctgtcgtga tgcccatgta aaaaatagta aaaccacttt
    21301 ctctttttgt tgatcctact ggatattcat tacctagtaa tgaagaaata ttaggtttta
    21361 ataaacctgt tcctacagct actcctgcaa gtgaagaggt taatgccagc gaattatcaa
    21421 tcaatgacaa catgcaataa cttaaaaaca gaacaatagc acccagcaat atagcgcgtt
    21481 tttggcctat taatttatca gcaatccagc ctcctactaa tggtgagagg taggtgagtg
    21541 ctgtgaacga tcctactaaa gaatagattt ctttgtccgg ccatttaaag tgtaaggcca
    21601 aataaagtgc taataaggat tgaactgcgt aaaaaccata acgttcccac atttcggttg
    21661 taaagtagat gcggagagat tttgggtgtt tttccatatt atttaagtaa ctacgattaa
    21721 attaatttat taaataaaaa ttatatcata taaaaaacag tatgaatatt gattggtcat
    21781 tttatctggt ttttctcaac tcattagatt atctcattcc cggaggtagt tgactggtgt
    21841 atgatgctaa aaaatttttt aatcgctatt tgatgggaaa taataactga agttttcata
    21901 gcaataagga gtattcttga tgaacaagga caagaataaa cgtgttattt ctgcccctag
    21961 aggtaccgaa attcaagcta aaaattggtt aactgaggcg gcattaagga tgatttgcaa
    22021 taatttggat cccaatgttg cagaggatcc cgattctttg attgtttatg gtggattggg
    22081 caaggcagcc agaaactggg aatgttttga tgaaattgtt catgtattga aattattaaa
    22141 taatgatcag actttactca ttcagtccgg aaaacctgtc ggtgtattta ctacccatga
    22201 agaggctccc agaatactaa ttgccaattc taatttggta ccgcgctggg ctacttggga
    22261 gcattttaac gaactggata agaaaggcct gatgatgtat ggccagatga ccgccggcag
    22321 ctggatttat attggttctc aaggcattgt gcaaggaact tatgagacat ttgttgcggc
    22381 agctaaaaaa cattatcagg gtgatttgtc cgggcgttgg attttaacag cgggtttagg
    22441 gggaatgggg ggagctcaac ccttggctgg aactatggct ggtgccagcg ttcttgctgt
    22501 agaatgtgac agacaaagga ttgagaaacg tttacagaca aagtatctgg atcggtacac
    22561 tgataattta agtgaagcat tagactggat taatgaatcc tgtcgccgta aaaagccagt
    22621 ttcagtagct gttttgggaa atgctgctga aatatttcct caactggtta agttgggagt
    22681 gcagccttct ctggttaccg atcaaaccag cgctcatgat ccgctcaatg gctatttgcc
    22741 attaggatgg acattagaac aagctgttga aatgagaaaa aaatcaccag aagaggttgt
    22801 tgatgcagcc aagaaatcga tggctgttca agtgcatgca atgcttgaat ttcataatag
    22861 aggcattcct gtatttgatt atggcaataa catcagacaa atggcatttg aagcgggaga
    22921 aaaaaacgct ttttcctttg agggatttgt acctgcttat atcaggccat tattttgcga
    22981 aggaattgga ccatttcgat gggttgcatt gtcaggtgat ccagaagata tttatgcaac
    23041 ggatgagcga gtaaagcagt taattcctga cgctcctcat ttgcatcatt ggttagatat
    23101 ggccagggaa aaaatttcat ttcagggatt accagcaagg atatgttggg tagggttaaa
    23161 ggatagagca cgccttgccc tggctttcaa tgaaatggtt aaaaataaac aagttaaagc
    23221 gcctattgtg attggccgtg accatttgga ttctggttct gtggctagcc ccaatcgtga
    23281 aacagaaggt atgctggatg gcagtgatgc tgtttctgat tggcctttgc ttaatgcgct
    23341 gttaaattgt gccagtgggg ctacttgggt aagtattcac cacggaggag gggttggtat
    23401 gggcttttcg caacatgctg gcgttgtgat tgttgctgat gggacagaaa aggcagctaa
    23461 acgattagcc cgtgtgttac acaatgatcc tgcgacaggg gtaatgaggc atgctgatgc
    23521 aggttatcaa attgccaagc aatgtgccaa agagaactcg ctttggctac ccatggaatc
    23581 ttaaaaggag caggtttatg tcagaacagt ttatattgca gcctggtcag ttatcgctac
    23641 tttctattaa acaaatttta gatgaggaac tatcttgcgt tcttgctgag aattcctttg
    23701 aattaattag agcctctcac caaacggtta aaaaagtaat tgatgaaaag aaaactgtat
    23761 acggaatcaa tacaggtttt ggttctctgg caaaccagac catttcttca gattgtttga
    23821 aagagttgca gcgtaatatt gtgctttctc atgcttgtgg aacgggtaaa ttattgccgg
    23881 atgacgtggt tgccttgatt ttgcttttga aaattaataa tttgtctcaa gggtattccg
    23941 gagttaggct ggaattaatt aatgccttga ttgctttgtt taatcataaa gtatatccat
    24001 gcattccctc taaaggttcg gtaggtgcgt ccggagattt agtcccgctt gcacatttgt
    24061 ctttaccttt gttaggagaa ggcgaagtaa gacatcaagg tcaggtgatc agtgctgagg
    24121 aaggactaaa actggccgga ttgaaaccgc ttgaattgga agctaaagaa ggattagcac
    24181 tgcttaatgg cttacaggta tctaccgctt tagctttgtc agctctgttt attagtgaga
    24241 cattgtttga aactgcgatt atttccggca gtttatcagt agatgctgcc agtgggagtg
    24301 atgtcccctt tgatgacaga atacaccaaa ttcgaggaca tcaagctcaa atttctgctg
    24361 ccagtatgta cagaaattta ttggcaggta gtcaaattcg agagtcacac aggcattgta
    24421 atcgtgttca ggacccttat tctttaagat gtcagccaca aatcatgggt gcaattcttc
    24481 atcagatgca gtttgttgga caaactctcc aagtagaggc caatgcaatt tccgataatc
    24541 ctctcgtttt tgcagagcag ggggatattt tgtctggggg aaattttcat ggtgaaatca
    24601 tcgcaatggc tgctgacaat ttggcattgg cattatccga gataggaggt agtgcagaac
    24661 gccgaattgc tttattaatt gataaaaatt ttagtggatt acccgcgttc ctggttagag
    24721 aaagcggctt aaattcaggt tttatgattg cacatgtaac tgccgcttct tgtgccagcg
    24781 ataacaaagc gcttgctcat ccacactccg ttgatagttt gcctacctcg gcaaatcagg
    24841 aagatcatgt ttctatggca actagtgcag cacgtcgact gcatgaaatg atcgataata
    24901 cttcaacaat tcttgctatt gagttattgg cagcttgtca agggctggag tttcataaac
    24961 ctctaaagac atcaccgcaa ttggataaaa tttatcaaag cgtaagaagc gtggttaaag
    25021 agtatgataa agatcgctat tttgcacctg atatagaaaa aattaaaaag aagattttgg
    25081 ataaagaatt ctcattgctc acactcacga acgaatagca ggaaaaatac aatggaattg
    25141 atggcagaat atctaacttt gcatcaacaa cacaaaaaca atccttatcc ttctttgtct
    25201 gaaagaaaaa aattgctgat tgcaataaaa aaaatcttgc agacagaagc ttacactttg
    25261 gcagaagcaa taaataagga ctttactcat cgctctgttg aggaaacctt atttttagaa
    25321 gtttttccaa cgataaaagc cattaatttt tgtttaaaaa atatgaagaa atggatgaga
    25381 aagagacgca gaaatgtgtc ttggttgttt attcctgcaa aagcctatgt tataccgcaa
    25441 ccccttgggg tggttggtat catggttccc tggaattatc ctgtttatct ggctttagtc
    25501 cctgctattt atgccttggc agctggaaac agggtgatga taaaaatgtc tgagttatct
    25561 ccgcatattg gcgatacttt actaaaattg atgcacgcgg ctggattaaa ccattctatc
    25621 agagtcatta acggtgatat tgagctatca aagcagtttg cttcgcttcc ttttggccat
    25681 cttatgttca ctggctcaac taatgtagga aaaatggtga tgaaggctgc cagtgataac
    25741 ctgattccag tgacactgga gctaggcggc aaatctccgg caatattatc tcccaccatg
    25801 aacccagctt attttaagcg cttgtttatg ggcaaattat tcaatgctgc tcaaacttgt
    25861 atcgcacctg attatttatt gattcctaaa ggatgggaag acagagtcga aagagaattt
    25921 aggaagttta ttaatgcctt ttacccggat ttgatgagta atgagcagta ttccagtatt
    25981 atttcagaga gacataaaaa gcgattactc gatctggtcg aagatgcgcg aagcaaaggg
    26041 gctcgtgtag ttgagtttgg tcattcaatt ccgaattgct caaagttacc tgtatttcta
    26101 ttatttgata taacaaatga tatgcttgtt atgaaggaag aaatttttgg tcctatactg
    26161 cctgtattga cttataactc aataaatgat gcagtcgatt acattaactc atctcccaac
    26221 ccacttgcct tgtattattt tggagaagac aagagtgaaa taaaaatcat acaaacaaaa
    26281 acattatctg gtgctttgac tatcaatgaa accttaatgc atattgcaat tgatgattta
    26341 ccatttggtg gtgttggtca tagtggcatg gggcattatc atggaaagga aggatttgat
    26401 accttttcaa aattaaaacc ggtgttagtg caacgattta tatctacagt tagctggcta
    26461 tatccaccct atggtgcttt aatgcgtatg tttttagctt gggttggtgg tatcaagttg
    26521 aaggagaaat catgagtaaa gtcattttta ttacaggtgg cagtagagga atcggtagag
    26581 aaatcgcttt aaaatttgct gcaaaggaca aagcaaatat cgttattgca gccaaaacag
    26641 cagaacctca tcctaaactg gaaggaacta tttattctgt tgcaaacgaa attgaagaac
    26701 tgggaggaaa ggctctgcct ttaatggttg atgtcagaga tgagcaacag atccaaaatg
    26761 cgatcactaa aaccatagaa acttttggaa gattggatgt attagtcaat aacgcgagcg
    26821 caatcaattt gactgataca ttaaatactc caatgaggcg ttatgatctc atgcagagcg
    26881 ttaatgtcag ggcaacgttt gcatgttctc aagcagccat tccatatctt ttaaaaagcg
    26941 agaatccgca tattctgact ttgtccccac ctttaaatat ggataaaacc tggtttgcac
    27001 cacatctggc ttataccatc agtaaatacg gtatgagcat gtgcacatta ggcctcgctg
    27061 aggaatttaa agaggcggga attgctgtta attctttatg gccgaaaact actatagcta
    27121 ctgatgccat aagagttcat tttcccagtg agctgtatat ggcaagcaga aagccactca
    27181 ttatggctga tgctgcttat tggattatca atcaaccatc aaaatcaatc actggaaatt
    27241 tttttattga tgaagacgta ttaaaaaata gtggtgtcac tgatttttct tgctatgcta
    27301 tgaatcctaa agctcaactt tgtcctgatt tatttttatg aaggtagaaa tctatactga
    27361 tggtgcatgt aagggaaatc caggacctgg cggctggggc gtattactcc gatataatgg
    27421 tagagaaaaa actcttcatg gaggagaggc gcaaactacc aataatcgca tggaattaat
    27481 ggcagcaatt aagggcctgg aagcattaaa acgtccttgc gaggtggatt tgtataccga
    27541 ctcccaatat ttgcagcaag gaatgaagga atggatcaaa acatggaaaa gaaatggttg
    27601 gcgtaactcc aagaaagagt tggttaaaaa tgctgaatta tggaaatcat tagataatct
    27661 ggcttctatt cataatattc attggcattg ggttaaaggg cactccggcc atttggaaaa
    27721 tgatttggtt gatgctttgg caaatttggg tattgaggaa ttatcataga gttatgagac
    27781 aagttatatt agataccgaa acgactggta ttggacctga acagggacat agagtaattg
    27841 aaataggttg tattgagctt atcgatagaa aattaaccgg caagcacttc cacatatacc
    27901 ttaatccaca gcgtgaggtg gatgaagggg catttagagt tcacggtata agtactgagt
    27961 ttttgcaaga taaaccttta ttccaggata ttgtgactga atttatacaa tttgtggaag
    28021 gttcagaatt aattattcac aatgctccat ttgatgttgg atttcttaat tctgaattaa
    28081 aacatgtgaa atggaataag acattagaag attattgccg gattcttgat actttgattt
    28141 tagccagaga aaaacatcct ggacagcgta atagtttgga tgctttgtgt aagcgttatg
    28201 aaatagatca ttttaatcgt gaactgcacg gtgctttgct tgatgccgaa atattagctt
    28261 atgtgtattt ggcaatgaca ggtggccaaa gcagtttatt tactgaggtt gaatccagtt
    28321 tgagtcaatc aaaaataaaa actcaagaga taaagtcatt aagtttaaaa aagccagtaa
    28381 taatgaaggc taatgaagag gaattaatgc aacatcaaag cttcgttgaa tttatatcta
    28441 aaaaatccgg tatcaatcat tgggaagagg tatagctctc atgattatat aaattataaa
    28501 taattattct gttacactta ttattcggtt tcgcgtctgc caatactaaa ccttatacaa
    28561 taacaaaatt cttcaggcat cttattcctg gtcgctatcc tcaaaataag caaccaaagt
    28621 gtttggttat aggcagaggc gaagcctaat gattatctca atcaagcaat ttttttatta
    28681 tctatttggg aattagatgt ttttcagcaa aatcttttcc gatacattct cctaatgatc
    28741 tcccccatgt gccagctgtt tcactattga ttttatctgg catgctggca tacagttcat
    28801 cttggcattt tttagtgctt tcaggaatta aagtcacgca ttgttcataa gtcattttaa
    28861 tttcatcaaa tcgttttttg agatccggat cttgaataaa tcctttgcag ataagatctg
    28921 gtaataaggg agtcatagag tttaaccagg catccttagt catctcatta ggatttgctg
    28981 tgtcttcagc attggatatt aagggaaata atgaagtgct tagcacgagc gtggtagcaa
    29041 taagtaattt ccttgacatg atttattgtc cttattttta tagtctcata tatatagtag
    29101 gacaaaaaat ataatttttc cttcattaag caatttaaag cactttagtt ctcaatgaca
    29161 gattcatgga gttacgacaa tcatctgatt aacatttgaa ttgttcaaaa attaaactaa
    29221 aataataaag tgacggaaaa aaaataggtt aaatcatgga aaagtccaat tttcgcttaa
    29281 attttttgtc tcaaatttta ttcttcactt taggagcatt tctattctgt aatgggtacg
    29341 cggcgggtaa tacctttatt tttaatccta atacattaac ctggaaagct atcagctcta
    29401 atggcaaggt gatacgtaca gggcgaggat caggtggaaa acactattgt tctgatgttc
    29461 gcagatcatg caaaacacca tctggtgttt ttagagtgat tagcaaaggt ggcccagcat
    29521 gccgatccag tcgttatccg ctgggtaaag gtggagcacc tatgccatat tgtatgtttt
    29581 ttagtaaatt gtatgcaatt catggttctc cggatgtgcc aaattataac gcaagtcatg
    29641 gctgtgtcag agtgaagcct catgatgcaa gatggttaag tcaaaatttt attaaaattg
    29701 gaacgaaagt aattattaaa tcctattaat agcattaatt aatgcgccat aattagggat
    29761 tatgtagtat aattggaatt attggaaaat tattattcac acgttcattt atactcatta
    29821 attttagaag aagtagcgtt atgattgaag taaatatttg gttatcaact gcttttttgt
    29881 ttaagaaacg aattaagcac aaattttttg gcccgctttt agcctcagag gataaagggg
    29941 aaaatgtagg acacgttaat tttactatag aaattgatga gcgtacaaaa acaaaaaata
    30001 gttttgattt tattgagcaa catgggtcag aattacgagc taaaaaaaca ttgcgcgtcg
    30061 taccaactaa agctaccaac acatctcctc caaacataga atcatctcat ttgataccaa
    30121 ctatagtaag aagtgatatt gtttctcata gtttttggcc agacgcgaga cccactaaat
    30181 cagaaaccat taaaggcaaa gctgtcaaac ccaagtttaa aactcatgaa gaagatatga
    30241 tttctgaaga ttcggtaaca gcaatgacta ttatccatcg aaaatctgct ttagatgaga
    30301 ttagcaggga aaagatggaa gatcttgatt ttcttgttga aatttcagat ttggaagtca
    30361 acttggagca aagaaaacta tttatggatg accttaaaaa attgcagttg gaaaaagatg
    30421 aattgattaa acaacagaag caattaacca attcttacca aacccaatta aaagatttaa
    30481 aggggcaatt agccaagctg gaacttcatt tgaataaaac aaacggtcaa attacagcta
    30541 cagagagaac tataaattat ttaaataaac tgaaaaatcc tgatcctaaa accaaaacac
    30601 aattgattga gcttactgaa aaattaatta aattaaaaca agagttcgag actacaataa
    30661 attctcaaca tgagataagc gcattgatat caaaatctga actgacttat catgaggact
    30721 tgcaacaagt tgaaaagaaa ctgcaacaga ttgagcttgc gatgaaccat cgtatagaag
    30781 atttgaaaaa gctggatatt aaaatcaatg gcagggatga gaatgattta aaagagttgc
    30841 aagaagaggc gcgtcgtcgc aaagaatata cttctcgcaa agaacaattc atcaaaagcc
    30901 gtgattttac tgaaggaagg caacctgatt acagcgttac tttgccaact gcagaaagcg
    30961 gtttaactta ttatgttgat gaaatcaaaa tactgaaagc tatgcaagaa gaacgcagcc
    31021 aaaactattc atttcttttt aataactgtg ccagtagtgc taaacgttgt ttgctagcag
    31081 gaatagatga aaaattgcga gccaagttaa aagaaacagg tttaggtagc aaattttttg
    31141 aaatcagtaa agtagaaaca tgcaagtcat tacgtgattg ggcaagaact cttgatagta
    31201 aattgacaga acttaatctt cgatctcctg ctcctacccg aggtgcttaa gaataataag
    31261 cagttcgtcc gtaaaaagct aagatcactg ccttgagttt caggcagtga tttttttata
    31321 aatcatcttc atcaccaata ctaaacgaag agccacaacc acaagttgtt tttgcattag
    31381 ggttgcgtat gacaaattgc tctccttgta ttcctttaat gtaatcgatt tccgcatcat
    31441 gcaaatattg ataactcatt gagtcaacta gcaatttgac agaggatttt ccatcagaac
    31501 attgttgtat cacaatagtg tcgtcatcat tgatttgttc atcaaagcta aatccgtatt
    31561 gaaatccaga acaaccaccg cctgtaattg aaactcgtaa attcaaattg gaattatctt
    31621 cttctttgat tagttctgcg actttatctg ctgcactgac agaaaaatgt atatctgaag
    31681 tattttgtga tacgtcaaga ttggccatac taagctccaa aataattact acctctacaa
    31741 ttcattatat caataataca agttgtttcg tagtctcaat ccataaattg gtattaggga
    31801 ccagagaaaa caaacagtgc caatacaaaa ttagcttccc tgtttagcgg ataatctgct
    31861 ctatagtagc tccgcccaag cattgatttt tttcataaaa gacgatgaat tgccccggag
    31921 taatcgcacg ttgaggactt gaaaacatca catagtgttg attattatcc ggaggagaaa
    31981 tcatgcaggc ttgatcggtt tgcctatatc ttgttttagc aaagcaggtt agtggtaaat
    32041 gattctcata gtctgccagc caatgaatgg gaccacatat caaaccttga gaatagagca
    32101 ttggatgttg actgccctgt gctacgtaaa gggtgttact ggctatgtct ttatccacga
    32161 cataccatgg ctcatctgtc gagttttgta atcctccaat tcccaaaccc tggcgttgtc
    32221 ctaatgtgta aaacattaaa ccatcatgtt gaccaagtgt ttttccccct gtacttttta
    32281 tttctcccgg tttggcaagt atgaattcat taagaaaagt tttgaatctt ttttcaccta
    32341 taaagcaaat acctgtagaa tcttttttgg catgagtgac aaggccaagt tgtttagcga
    32401 attctctaat ttgtggctta gtaaaatcac ctattggaaa aatggttttg gacaatgcct
    32461 ctggttcaac agcgtggagg aaatacgttt gatccttttc cctatctttt gccttaaata
    32521 aatagccaat agatccttct attgtatttt tggcatagtg gccagtagct atataatctg
    32581 cgccaagggt taaagcatga ttcaaaaagg cattaaattt gatttcttta ttacataaca
    32641 cgtcagggtt tggtgttctt cccttttcat attcactcag aaaataagca aatactctat
    32701 cccagtattc ttttgaaaaa tttacagtat gtagtggtat gcgcaattga ttacagacgg
    32761 cctgggcatc tgccaaatct tttgctgcag ggcaataatc attgtgatcg tcctgctccc
    32821 agtttttcat gaatagtcct tccacctggt atccttgttc tttcaatagc caggctgcta
    32881 cagaagagtc tactcctcct gacataccaa caataacttt agctttcata aatggtttgt
    32941 atcgctcttt tttgaatcat aatcatattg gaatgtgcga ttttaacaaa ttaactacac
    33001 tctaggaaat tgtaatttag ccctgtattt attacccaat aagttgatat tttttacata
    33061 taatcggata ataccacgtt tttagggatt cgttatcatt ttaacttgat tatgttagta
    33121 agtcataaac cgtctataag tgcttatata aacttgcaag actcaaagag caaactcatt
    33181 ctatatttcg gataattatc tatgatgcat ttacaagagt tggcaaaaca tggccagcaa
    33241 gaaaaggaag tgattttaaa cgagcgttta ccctcgtttg ttagtcccgg atgccatttg
    33301 catgcatctt atcatataga ggcaaaagat gatttttatc ttattcatct tagagtgcaa
    33361 ggcaatttaa acagcgtatg tcagcgatgt atggaaaact tcactgtatg ttataataac
    33421 tcgactgtta ttgctgtttg ccgaaatgat gagagagctg agcaactttt gagtcaatat
    33481 gaatgcttgg tttcttcaaa ctggcaagtt gatttaaatg aattaataat tgatgagctg
    33541 catttgtatg caccacaagt tcatcctgat attaatgaat gtgctaatga aatcaatgaa
    33601 attttaactg ggaaaaaaca aacttcttaa tattttgata attaatactt ggattgaagt
    33661 taaaatatcg gtaatattcc cgctttatga atgcaatttg tttaggagta atacaatggc
    33721 cgtacaacaa aataaaaaat cacgctcacg tcgtgatatg aggcgttctc atgatgcact
    33781 aaccaagcct actctatcag tagatccaac aactggtgaa acccatcttc gtcatcacat
    33841 gaccccagat ggatattaca gaggcaaaaa aattattgac gctgaaaccg cttacgaaca
    33901 agagtaactt tcttgaagaa tatcaccatt gcaattgacg cgatgggtgg ggatcacggc
    33961 ttggaaattg tgattcctgc ctgtattcgt gctattaaaa acaaccctga tttgaaacta
    34021 ttgcttgtag gagtccagga caagatcagt gcttctttga aaaaacatgg catgttgtcc
    34081 tgtcagcaat ttactatcgt ccacgcatca gaagtggtta caatggatga attgccatct
    34141 catgcattac gaaataaaaa agattcctca atgcgaatag caattaacct ggttaaggaa
    34201 ggcagagcgc aggcttgcgt cagtgcgggc aatacaggtg ccttaatggc taccgcccga
    34261 tatgtcttaa agactttacc ggggattgat cgcccagcaa tagtatccga gttacctacc
    34321 atgggtggaa aaacaagagt cattgatttg ggagccaatg ttgattcttg cgcagagcat
    34381 ttgtttcagt ttgctgtgat ggggtctgca ttgatacagg caatagaaaa taaacccaaa
    34441 cctaaaattg gtttacttaa tattggcgtt gaagaaatta agggtaatga tcaggttaag
    34501 cgtactgcac atatgctggc tgagtgttct gttatgaatt atgtgggata cgttgagggt
    34561 gatcactttt acagtggtga tgttgattta gtggtctgtg atggatttgt tggaaatgtg
    34621 gcattaaaag caagtgaagg tttggcaaag cttttattaa ctgtccttaa agaatcattt
    34681 agcagaaatt ggttaacaaa aattgctggt ttgattgctt taccagcctt aaaacattta
    34741 aaaaatcgtt tggatccatc gcgatacaat ggtgccagtc tactcgggtt gaatggtatc
    34801 gttgtcaaaa gtcatggtgg agccaatgaa gtgggttttc aacatgctat tgaacaggct
    34861 gttcttgaag taaaaaataa tgttgtcgat ttagtgcgtg atcaaataaa tgattttata
    34921 aatcaaggat tgttgttatg aaaaatgctg ttattaatgg cactggaagt tactctccag
    34981 agagacaaat gactaatgct gaactggaaa ccatgcttga tactagcgat gaatggattg
    35041 ttaccaggac tggtattagt agccgtagtg ttgctcaaga acatgaaaca acatcttata
    35101 tggcttccag agcagcagag caagcactag aggcatcagg ccttgatgct gaagaaattg
    35161 atttgatatt agtagcaaca tgtaccccag attatttttt tcctagcgtt gcctgtcacg
    35221 tacaacatgc tttaggaatc aaaagaccta ttccggcttt tgacattgga gctgcatgca
    35281 gcggttttgt ttatgcgatg gatgtagcga aacaatacat tgctacaggg gctgccaaac
    35341 acgttcttgt cgtaggcagc gagagcatgt caagagcggt agattggact gatcgttcta
    35401 tttgtgtctt attcggagat ggcgcaggcg ctgttgtttt aagcgcaagt gatcgccaag
    35461 ggattatggg tagtgtttta cattctgcct atgactctga taaattacta gtccttcgta
    35521 attcaacttt tgaacaagat cgtgcaacga ttggaatgcg aggtaatgag gtattcaaaa
    35581 ttgctgttaa tattatgggt aatattgttg atgaagtatt agaagcaagt catttaaaaa
    35641 aatctgatat tgattggctg atacctcatc aagccaatat acgcatcata caagccatag
    35701 ctaaaaaatt atctcttcct atgtcacatg ttattgttac aattggtaac caaggtaaca
    35761 catcggctgc ttctattccc ttagcacttg attattctat taaaaataat cagattaaaa
    35821 gggatgaaat attattaatt gaatcctttg gtggtggaat gacctggggc gctatggtta
    35881 ttcgttacta aattaactaa taaggattaa ttctattatg gtaaaatcag catttgtatt
    35941 tcctggacag ggatcgcagt ctatcggcat gttgtctgac tttatgccac agtatactgt
    36001 tgtcaccgat gcttttgcag aggcctcaga tgctttgggc tatgatttgt gggatttggt
    36061 tcaaaatggg ccagagacaa agctaaatca gactgaatac acgcaggctg ccatgttaac
    36121 tgctgatgtt gcaatttatc gtttattaat gcaattaggc gtacctcagc ctcaagtaat
    36181 ggctggtcat agtttggggg aatacgctgc tttggtttgc gctaattctt tatctttaaa
    36241 ggatgctgcg cagttagttg ctcgtagagg acaggtgatg caaaacgcga tccctctggg
    36301 agagggggct atggctgcca tagtaggctt atctgatgaa caggttcgtt cattgtgtga
    36361 gcaggccagc acagccaatc atcttgttac cccggcgaat tataatgctc tgggccaaat
    36421 tgttgttgct ggacatcgag tagccgttga taaactaata caattggctg aagaggctgg
    36481 agccagactg gcaatgataa ttcctgtaag tgtgccttgt cattgcccct tattaaaaaa
    36541 tgctgccgaa ttatttgctg aagatcttgc tcaaattgaa tttcaagttc cttcagtcga
    36601 tgtaataagc aatgttgatt taagtattta tcattccact caacatataa gagataaact
    36661 caaagagcaa ctatacagcc ctgtgcgctg ggttgaaaca gttcagttaa ttcaacagcg
    36721 cggaattgat ctggttgtgg agtgtgggcc aggaaaagtt ctgaacggtt tgataaaaag
    36781 aattgagaga aatttaacta cgattagcgt ttatgacacc attagtcttg atcaagtcat
    36841 agagcgttta catatcccag tttgagagga aactagatga tcaatttaga aggtaaaatt
    36901 gcattagtta ctggtgccag ccgtggcata ggtcgtgctg ttgccgagaa attggcaaga
    36961 aatggtgcct ttgtttttgg aacagcaacc actgaacagg gtgctgcgtc tattaattct
    37021 ttttttgaaa aagaaaaatt ggctggtaaa ggcatcgttt tggatgtaac cagcactgag
    37081 caagtagaaa atttgatgtc taccttgtct gataatgatc agtgtccttc tattcttgtt
    37141 aataatgctg gcattaccgc agataacctg ttattgcgta tggatgatga tgaatggtac
    37201 aaagtaatag aaacaaattt gaattctatt tacagaatga ctaaagcttg cttaaaacct
    37261 atgttcagag cgcgttgggg aagaataatt tctataggct ctgtggtagg tgccagtggc
    37321 aattctggac aggtaaacta tactgcagcc aaggctggag ttattggttt ttccaagtct
    37381 ttggcacagg aaattggtag tagaggaata actgtaaacg tggtagcccc cgggtttatc
    37441 gacacagata tgactactgc attgcctgat atggtgaaag aagaaatgtt aaaaagaatt
    37501 cccatgcgta agctgggcca gccagaagat gttgctgatg ctgtagcttt tctagcatca
    37561 gacagcgcaa aatatatcac aggtgaaaca attcatgtca atggcggtat gtatatgagt
    37621 taaatgcact tgcgttatct gtataattga ataaactagc cgtcagtatt taataatttc
    37681 tatcaaccga aagaggaaaa ttacgttatg agtacagttg aagagcgtgt tcgcaaaatt
    37741 gttgttgaac aactgggtgt taaagaagaa gaattaaaga atgatgcatc ctttgttgat
    37801 gatttgggtg ctgattccct ggatacagta gaattggtta tggctcttga agaagaattt
    37861 gaaacagaaa ttcctgatga aaaagctgag aaaattacta caattcaaga agcaattgat
    37921 tatattgaat ctaacttaaa taaagaagaa gcttaatttt tttaaggagc tctcattgaa
    37981 tagtcggcgt gtggttatta ccggcatggg tatgcttact cctgtcggac ttaatgtaga
    38041 agaaacttgg cgaagtattt tagctggggt tagtggggta ggtttggtgg aagattttga
    38101 tactactgaa tacccaacta agatttgggc taaggtcaag aattttaatg ttgagaatca
    38161 catgccaatt aaagacgccc gcaaaatgga cgtctttaca caatatggta tggctgctgc
    38221 tgatgaagcg atgcttgact cgggtttaaa aattgatgaa gtcttatctc gtcgaatcgg
    38281 tgtcgcagtg ggcgcaggta ttggtggtat tcaaacaatc acaaataacc aggataaact
    38341 ggttgccggt ggaccacgta aggtttcgcc attttttatt ccggcaggta ttatcaatat
    38401 ggttgctgga cagatttcta ttaaacataa tttaaaagga cctaatattt cggttgtgac
    38461 tgcatgtact accggaacac ataatatcgg gctagccggg cgtataattg cttatggcga
    38521 tgccgatgtc atgatttgtg gtggagcgga aatgaccaca acgccacttt gtcttgctgg
    38581 gttttcagca gtaagatctt tatccaaacg gaatgatgag cctgagaaag cctcaaggcc
    38641 ttgggataaa gacagagacg gttttgttat gggtgagggt gctggtgttt tggttttaga
    38701 agaatatgaa cacgctaaag ctcgtggagc caaaatttat gctgaattgg ttggttttgg
    38761 tatgtcagga gatgcttatc atattaccgc acccgatgaa gatgccgatg gagcttcacg
    38821 agctatggag gcagccatcc aggatgctgg tatcgatccc agacaagttg attatattaa
    38881 tgctcacggt acgtcgactt atttaaacga tttaaatgaa acaaaagcca ttaagcgtgt
    38941 tttccagaac catgcttatg atctggcggt tagctcaacc aaatccatga caggccattt
    39001 gctcggagct gcaggtgcag tagaggcaat tataagtatt ttagccataa gagatcaggt
    39061 ggctccacct acaataaatt tggataatcc tgatgaaggt tgtgatttga attatgtagc
    39121 acatgtacca caaagcagaa caattaatta tgttctaagt aattcacttg gttttggtgg
    39181 gacaaacggt agcttactgt tcaagcgaat ctaaatgcaa ttctctagac ataaaaaact
    39241 ggttattctt gttttatcct tgttttttat gtctttcttg tttttatttt ggaatatata
    39301 tcgcttattg tatacaccaa taattccgaa aactgcttct ccaatgattc ttgccttgga
    39361 taaggcgacg tccgcttatc aatttgctta tattttgcaa gataaaaaat taatacattc
    39421 tgccaaattt tttctattga ttattcgttt tgaaggatta tcgcatcagt taaaggcagg
    39481 cgtgtatcaa attactcctg gcgaaactgc aatgaaatta ctacatcgag tagttgctgg
    39541 tgatgtaatt acgcaaaatt ttacgatcat cgaaggatct acacagcaga aagtagatta
    39601 tgacctaaga caggctaatt atttgaaata caatccagaa gattgggcaa tagtaaaaga
    39661 aaattaccca agtgcagagg ggttacttct agcagacact tatcaatacc aaggtgggag
    39721 tagtagtagg gcattattag agcaagccca tcgtaattta ttaaattatc ttaataccag
    39781 ttgggcaaac agatcaccaa atttgcctta taaaactcct tatgagctat tgattgccgc
    39841 gtcaattatt gaaaaagaaa ctgcaattgc acaagagaaa aaattgattt ctggtgtgat
    39901 ggtaaacagg ttaaagaaaa aaatgccatt acaaatggat cctacggtta tctatggact
    39961 gggaaatcaa tataaaggaa aattaacaca taatgatttg ctaattcaat caccatataa
    40021 ttcttatttg aatagagggt tgcctcccac cccaatagct atggttggca aagaggccat
    40081 agatgccgct gcacatccgc aattgtccaa ttatttatac tttgttgcca aaggagatgg
    40141 tactcatcaa ttctccgaaa cttatgagca acaaagacaa gcaattaacc aatacagacg
    40201 caaggattat taatgtcatc gttaaccggg aaattgatag ttattgaagg tttggaggga
    40261 gcaggaaaat ctacagctgt caatttagtt gttgaactgt tatcccaaaa aaaaatcagt
    40321 actataacca cgagagaacc cggaggaaca aaaataggtg aaattttaag aagtattatt
    40381 aaaaatccag agtacaataa tgttctggat gacagaagcg aattacttct cctgtatgcc
    40441 gcaagaatac aactgattga gcaagtgatc aaacccgctt taaatgtggg gcagtgggtc
    40501 atagctgatc gatttgagct ttccacttta gcttatcagg gagggggccg caaaatggat
    40561 atgcgggtta ttaatgagct atcgaatttc tgtttaaaag gattcaaacc tgatcttact
    40621 ttatatttag acattaatcc cgagcttggc atgataagag ccaaatcaag gggaaaattt
    40681 gacagaattg agcaggaatc tattgagttt tttcatagaa tacatgatac atatcatatt
    40741 cttgttaaac aaaacccgga aattataatg attgatgcta acaggtcttt ggaagaggtt
    40801 caatcttcca ttcaatcagt aatcgaggag tttatagagc ataacctatg attacttatc
    40861 aagaacaatg gcagaaaatg caaatggcct tggcgataag acgaattcct caagccatgc
    40921 tattttcggg ttctttgcat tgtggtctta tagaatttac aaatcaagtg atcaaattac
    40981 tcttgtgtaa aataaaacag aatgagcctt gtttgacttg catggattgc caaatgatag
    41041 agcgttttga gcatccggat gtggaatgga ttaagcctga aagaagcggt ggtattatta
    41101 aaattgatca aattagggga ttgcagagta cggcgtttct aacaccacag aggactaatc
    41161 ataaacttat aattatcgaa tcagctgaat caatgaatat tgctgcatca aatgccttat
    41221 tgaaaattct ggaagagcca gcgcaacata ctatctttat tttgttagcg caacaaattg
    41281 gcaatatacc ccccaccgta ttgagccgat gccaaataac gcaatttgct gtttttgatg
    41341 agttgccttg caacaattta ttggctttag gagagtacta tactcaggaa ttgaatagca
    41401 caatggtctt caagcattca gaatctattt tagaaggctt gattgcaatt attgagcgaa
    41461 gagagcatcc atgcactttg gctgcacaat ggattcaaca tgaattaagt gcttttttgt
    41521 ggtttttata tttgatctat tctcaactac agtatatgca tttgtttcga gtggcttatt
    41581 ctggtacgtt aacaaaacaa ttgaataagc tatcctcatt actcaatccg gttatgattt
    41641 ttgcccaagt agataaaatc aatactttgt taagaaaatt aagccataat atgaatataa
    41701 atcatacatt ggtcttggaa gatttattat tttctttggt tgttgattat taattgagtt
    41761 taaaggaatt aaaccattga gtgtaaaccg agttttttat taagcaactg aagtgagcaa
    41821 tatgattagt taaaacagga ctattcaagg aggatgtcta ttgattgaac ggaggagtta
    41881 tgaccgaaaa accacagatt ataaactgct catttcccaa tgaagcatct ttgtacatgg
    41941 cttacatgcc ttttgtgaaa ggtggcggtc tttttataag aactcaacac gtttattctc
    42001 tagggcagtc gcttgaatta tctgttaaat tgctcaatga aaatgaacca tatttggtca
    42061 ctggtaaaat agtctggatt actcctaaag gtgcacaagg taataagccg ccgggagttg
    42121 gtattcaatt taccggtgaa aacagtcgtt atctatgtaa caagatagaa acttatttgg
    42181 caggaatgct aaaatcatgt caggttacag atactatata gagttttcat ttattcatga
    42241 acaatatatt cattgatatt attttggggt ataaaggtgc tggtcgactc tcattgtcat
    42301 ttaaatttta tcgatttgac tcattttaat catgatcttg ctcaggtaat caatcaggct
    42361 cgcgagaatg gggtggagta tttcttgtct gtttgtgttg agttgagcga ttatcctcaa
    42421 ttagagcgtt tagctaagga ttatcctgat attagcattt ctgttggagt gcatccgaat
    42481 agtgaaatga attatcctgt tacggcacag atgttatgtg aattggctca aaatcctgcc
    42541 tgtattgcca taggagaaac agggcttgat tattatcgta atcccactga ggaagctcaa
    42601 tctttgcaac gagatcgctt cagggaacat atcaaggcgg cacttataac ttccaagccg
    42661 ttaattattc ataccaggca agctgctgaa gatactttgt tgatgatggc taaggagggg
    42721 gctagtcaga taggcggagt catgcactgt tttgctgaag actttgatat tgcgcaaaga
    42781 gcaattgatt taaattttta tatatcattt tccgggatag ttacttttaa aaatgctgtt
    42841 tcattgcaag atgtagccag acagatacct ttagagagga tattgataga gacagattca
    42901 ccttatttgg ctccggttcc acacagagga aagcaaaatc atcccgcttt ggtgaaacac
    42961 gttgcagaaa ctatcgcaca actcagaggg atggatttcc aagagatagc agaaatcact
    43021 acaaacaatt tttataattg ttttaatatc aaacgacaag gataaatcat tggtaaatcg
    43081 tagttctcaa cttcaaagca gaagcagtaa tttttttgcc tgtgaataaa ggttttagta
    43141 gtattgatat atcctattgc aaatttagag taattaatga gtttatatat cggtttgatg
    43201 tccggtacca gtatggatgg cattgatgct gccttgttgg agcttccttc caatcatttg
    43261 attcacggga ttactaaaca atacagtgat gatgtcagga gaaatcttga tgatttaatt
    43321 atgggtaatc atttaaccct ggcttcgatt tgccaactga acacgctaat tggcagggag
    43381 tttgcagaag cagttcgcca actcttgagt gaaataaaag tgcatccaaa agaaattcag
    43441 gccattggca gtcatggtca aactgtctgt catgatacaa gtggaaatat cccgtatact
    43501 ttgcagcttg gttgtggcca tacgatttct tctttaactg gcattaccgt tgtagcagat
    43561 tttagaacaa gagatttggt gaatggtggt caaggtgcgc cttttgcccc cctttaccat
    43621 caacagatat tttctaaagt gaatgagtct gttgctgttg ttaacattgg tggtattgct
    43681 aatgttacct ttattgcaaa gaatcaaatg actagaggtt gggatatagg gcctggcaat
    43741 tgtctgatgg atgcctggat ttataaaaat aaaggagccc tttttgataa aagtggggtt
    43801 tgggcgagcc aaggtgaagt gatttatcct ctgcttgaat atttgttaca agatccattt
    43861 ttccatttgg attcacctaa aagcattggc aaagaatatt tttctttatc ctggctacaa
    43921 aaacatttaa aaccggatta cacaccagct gatattcagg ctactttgtt ggcgcttaca
    43981 gcccatacga tagctgaaac aatattaaat gaatcaggag aaattaaaca attatatctc
    44041 tgtggaggcg gggcccacaa tacgcatttg aaagagaatt tagctcgtct actgcctggg
    44101 atagcagtaa aaagcatagc agagttaggg attagtcctg attatcttga ggcaatgatg
    44161 tttgcatggt tagcagcaca gacgattaat caaataccag ttaatttaac atcaataact
    44221 ggagctaaag gtattgcaat tttaggtgca gtgtatccta taataaaatc atattgacaa
    44281 actaaagaat cataaggttt aatttcataa aaattaacag agtgatttta aattgaccgc
    44341 acattacaac gtcgcttcaa acagtggagc aagaaataca cttgcaacgt cttattttat
    44401 tttttttcta gccgcttcat tttacttgta tgaattcata ctacaagtag caccaagcgt
    44461 tatggcagag tcgatgatga aaacctttgg ggtgacgggt caaggctttg gtgtcatttc
    44521 agctttttat ttttatgctt atgcccctac acaattgcca gcaggagtat tatacgatag
    44581 atatggacca cgcaagttaa tgacttttgc aatcgttttg tgtgcatttg gttctgcctt
    44641 ttttgcttcc actgacagtg tatttacagc ttgcataggc cgatttttga tcggaatagg
    44701 ctctgcgttt tcttttatcg gtgttttggt ccttatttca cgctggtttc ccccttatta
    44761 ttttgcaatt ctagcagggg tagcgcagtt gatgagttca gtcggcgcta tgtttggtga
    44821 aatgccgttg gcagctttaa ttgatctggt tggatggaga agtgcgagtt ttattttagc
    44881 gggtgttgga tttctcttgg ctattttatt ttgggtattt attcgagatt atcctcatca
    44941 gcaaaatcag acaatccctg atcattattt aagagatgaa tggaagcgct tagttgctgt
    45001 ctgtaaacat gcttatacat ggattatcgg tggttatgct tttgcaattt ggactcctat
    45061 tgcagtattt gctgcacttt ggggggttcc ttttttacag gaaaaatttc aagtaagcgt
    45121 agtggctgca tcaggcttat gcagtatgat ttggctaggc attggtgttg gtagtccctt
    45181 attaggttgg ttgagtgata aattggagag tcgccgtatt gcattgatta tcagtgcggt
    45241 tttaggactg attgcaactc tgattatcct ttattggtct gagttatctt ataattgggc
    45301 ttattttgtc ttgttcgttt taggattagg tgctggagga cagacggtaa gttttgcagt
    45361 agttaaagaa aataatacac cggaattagt tggcacagca tctggtttta ataatttgtc
    45421 tgtattaatt ggtggggcca ttttccagcc cctcgttggt tatatcttgc aacagacggg
    45481 atcatggcga attgtcaatg gtgctcatgt atacagtata tcgagttatc agactgcgtt
    45541 gcttgttatg ccagtatgct tcctggccag tttgcttatt gccacttttt tgcttaagga
    45601 atcgcatcct gcgcgcagag attaataaca gatttatcaa ttcaattttg cgttattgac
    45661 caaaaacaaa ggaatcagat tatgtaaatg tggcctgttc ttatgagtta gccacattac
    45721 tatttgcttc atcatgaatt tcagatttct tgggagtccc tataaaaaaa gttaaaccga
    45781 taaagattag aaataataat ccaacggcat tacaatagaa ggcgaaatgc agattattgt
    45841 gatgttgata aaacaggtta gctatttcta ttccaatagc cccaatgatc ataatactta
    45901 ggctgattaa tgctgaggct gttcctttgc ttacagcggt tacaaataaa caatacctgt
    45961 tcaagggggc attaattaca ctcagtgaga aaaagtaaat tatgattccc gggatcaaat
    46021 acacataatt attgccataa aaataaggga gaagagctgt cagagcaagt cccacgacca
    46081 ttatgataca acctataaaa ataattctct cgattttata tttgtaagtt aaatgatgta
    46141 agcaccagtt tcctaaaatt gttgccccga agatgggtag ttgccaaagg ccatattgaa
    46201 ttacagttaa ttttgcttca gcaattaata tgattggcgc cagcgcaatc caggcgatgc
    46261 atgggatccc taccagacct tcagcaattg cactataaca aaaggcctga ttagataata
    46321 acgttttata atttctggtt atagcgttca gtgcgaacgg tgttttaggt ataagctggc
    46381 cgtctctttt tacctggcct atgggttctg gcataaaccg ccataatccc caataagcta
    46441 aaagagcacc caaagcaata ataataaaaa tcaaacgcca ggacgtataa tgaatgatta
    46501 tggctcctaa tagtggccct agtaatgggg ctaatattgc ggcattagcc atgatagcaa
    46561 ttaatcggat agcgtccatt tcttcaaata tttcttgaat tgtggcataa cctatcacac
    46621 caatgaaaca caatcccatt ccctgaaaaa accgggcgat taaaaattga ttcatggagt
    46681 gtgagcttgc aatcaaaaga gtgaatagaa aaaataagca tgatcctatt aacatcattg
    46741 gtcttctgcc ataagcatct gatagtgggc caagtatgag ctgcaaactg gcccctccga
    46801 ggacatagac agttagagag gtggcaacaa cactttcatg cgcgttgaat gatttaacaa
    46861 cattgatcat acctggcatg atcatgtcat tggctatata agtcaaaaac tcatataaaa
    46921 ctaggaagca agcaaagata atggcctgtc tgtatgaaat tttaattagt ggctcagaca
    46981 tagcattaac cctgaatcaa tcgtttctaa aaggcggtct atcagtatat acgtttattt
    47041 catgatttgg tagaaaatct gataaatgtt ttggtaagcg acataaaatc tgccatatac
    47101 tgagaataaa tctttacaat agtaactgcc acttaaagtt tgtttacggc aagcccacca
    47161 tttgataaga tttgcttact tctataatct gcttcttatg atctgatcag tatgcccaaa
    47221 gaaaaagtcg ttattattat ccctacctat aatgaagaag ataatatttc taatacgatt
    47281 gatttgattc ttaaggagac atactcactc gcagattatc aggttgacat tcttgtattt
    47341 gatagtcact ctacagattc tacagcccat atagttcgag agctaactta tacctctcaa
    47401 cgtattcatt ttgcacaaga accacagaaa actggacttg gttccgctta cctgcaagct
    47461 atgagaattg caatagatga gttaaatgca gatgttgtat ttgagtttga tgcagatggt
    47521 tctcatcaac cttgttatat tgctccaatg ttagagctac ttaaacagca tgatgtagtt
    47581 gtggggagcc gatatatttc agggggaagg attccctcaa actgggcatt ccatcgtaaa
    47641 ttattatctg tgggaggtaa ttgggttgct cgtatgttat taactggaaa atataaagat
    47701 cttacttctg gcttaagggc aatacgtacc aatctgttaa agaaactatt gccttcaaac
    47761 ttcctatcca ataattacgc atataaactt gagttatatt ggcttttaca caaggcaaaa
    47821 gccaggatcg ttgaatttcc tattcaattt attgatagag aaaaagggaa aagtaaatta
    47881 cctaagaaca gtatgattga caccttgcgt gttcttttta cccttcgctt cagagaatta
    47941 cgccagtacc taactatgtg ttgtgtgggc tctatcggtg cattgattca attcagctta
    48001 tacaattggt tgcgcagttc gaaccattcc gctctggtat ccagccaggt tgctatcagt
    48061 gctgctatta tatccaattt tatttttcac cattccatta cattcaaaca aaaaactgac
    48121 ttccggataa ctaaaacaac atttagccgt tttacacaat ttattattta ttccacattt
    48181 atggtttatt ttcaaagcct atgggttcat atttttgtcc attcctttgg acaaggtgtt
    48241 ttgaaagaaa atatcattgt agcctcaggc ttgggtattg gatctattat caattacctg
    48301 ttttattcaa atattatttg gtcatcaaaa ggcactcatt ccctcaattc aaactcttct
    48361 aaagaaactc aaatttgaaa gcaaaagaaa ttgggggcat aagtcctgtt tcatttaagt
    48421 atccagctta ttactgcacc aataggcgta aatgattggc ttaccttttt catcaaaagc
    48481 agctggcaaa ttcaatgatt gacactgggc aaagtatttt ttaacacatg ataatctatc
    48541 aaatgtatcg atatatagca cttcgccaag ggtattcaga ttaggtgacc agtacctgta
    48601 ttgattttgc attgaaccgc agtcgaaggt aaaaattgtt ggtttatttt taagaaagaa
    48661 cagcatgcgt gcctcaaacc atccaggagt tatgactatg cttgggagtt tatcattaga
    48721 ctgattaatt tgctgtatcg attgataata agccaatttt tttgaatacc caaaactaaa
    48781 aagagtagta ttgttcatca aaatgattcc actggtaaga acgtagaata caattaataa
    48841 ataagtggaa ttacgataag cccactcttg atagcaataa ccagctaata agctggcagt
    48901 aattatgtat tggctcaacc aatatgctct tatcgtggct ttgccggcga caaataaata
    48961 gaaaagaaca aatgttacgc aaataatgag gcacaaataa aatacagggg attttctagg
    49021 ggtgcctttg gatagatata acgcaggggg aatgaacaaa aagttaattg cagggaggaa
    49081 aaaattaaaa aaggcatgca agataccccc catcgcatga tgagtatttt gcgattggtg
    49141 agtacttaac tggtagcgaa tggataccca atcatgttga gcattccata gaataaccgg
    49201 gctgaagaca atgattgcca gggttaatgc caaataaaaa tgcctggatt taaaaagccg
    49261 cctatagggt gttgcaacca aaaaaacaag taaggccagc acgagaacaa ttcctgagta
    49321 tttggataaa agcataaggc ctatactcaa acctatgtaa tacaaatcct ggtttcgatt
    49381 aaactggata taccttattg taaagtaaag agttaatgcc caaaaaaggg ttaaaggcgt
    49441 gtcataggta gtttgattga tcaaatctaa agtgactaat ggtgagaata accaaatcaa
    49501 aaccgtaaca taactggctt ccttattaag gcataatctt gctgttttat aaattataaa
    49561 actggtcaag gcagtagaaa ctatagtaac caggtttaaa gcaaagagag tatctccaaa
    49621 taaaagtgta gctatgcgga taaagtaggc aatcatggga gaaccatcat agtatgatag
    49681 agccaaatgc ctgctccaat cccagtaata ataactatct aatccaagga ggtgaattgg
    49741 tgcaattaat agaatgagta gaaaatagag gctaatgtag cgattcatgt gcaaaaacat
    49801 tcagtattcc caggaaagta atgatgaatt gtatgttatt ttattatcag tttttatata
    49861 atgcattcag ttttatcatt aattaataaa tgacgtttat gaaattatat ttttttggtg
    49921 atgataataa taaacccaaa ttaagcgaac atttaatcat ttacatactc agtttgctag
    49981 atcccaagtc tcagtctgtt gcttctttag tgaataaaga ctggtcccgg ctggttcaat
    50041 tgacacggca atacctgtct atgttaccgg tgattcatcg aataccttta tttgcgaatt
    50101 tgggtcttga cgttttgaaa ttaaaattaa tgacaggtgg catgacgaat tcaacagtta
    50161 ggctaagaat caaaaatgcg cctgagaaat gggttatgcg gattcctgga ataggctctt
    50221 cagcatttat aaccagaaga gatgaagctc acaacgctaa acaagccgaa aaacttggac
    50281 tgaatgttcc aatagctttc tttgatcctg aagatgggtt gcaggtaacg cgctttatag
    50341 aaggagtgca ttctctggat gataaagcct tggcccgcaa ggaaattctg catgaggtag
    50401 catctatgat gagaagactt cataaatctc ctccctttga taataacacg cttttttttg
    50461 agcgaaatga agagttattg gatttattaa aacgaaaacc ctttaatttc ccaggggaaa
    50521 tagaatttgt tgagcaacaa atgaaaaatc taaaggagat tttttcttct tatcatattc
    50581 agcaatttcc ttgccacaat gatacaacgc cacttaattt tatcttgtcc gaaaatccag
    50641 aaatcaaggg ttctgaaaag attcatcaga ttgattggga atattcaagt aataatgatt
    50701 ttatttggga tttggtttat tttatggttg aggccaagct aaaccgcgaa caacagctta
    50761 ttctgttgaa agcttacttt aatacagaag agctgagtga ctctctgttg gcatggatag
    50821 aggtttataa acccatcatt gagtggtgga taaccatttg gtcatggacg caattggcaa
    50881 atggagctaa tgcagttgat ttgagttctt atgagcaact agggcttgaa cggtatcata
    50941 acaccttaag tcacttgaaa agtgaggact atgtcaaagc cctggcactg attgaagcag
    51001 atagtcatag ccttactttt acggggcaga ggccttttta gctgccgcca ttatctagag
    51061 aggacaactc gtttacagtg acgattaaga tcttcagagc atttttttga gaaattcctg
    51121 taaatgcatc ttgtctttct cagcacctaa atagtttttg agttgtgcta tgtttctgtc
    51181 ataaccttcc tggctaattg tgccacgcat cagctcaaat cttaaataca cacaataggt
    51241 gttgagtaca tctgtttcac aataatctct tatttctttc aattggcctg cctggtattg
    51301 ttcccaaact ttagagccac tcattcccat ttttccaggg aaacctagca tggttgaaat
    51361 gtcatcaaga ggggcaaaag ctctgttctg atatcctgca agaacatcca ttagatcaat
    51421 atgtcggtag tgaaagcggc ttagataatt gttccatttg aaggtttgtt gattttctcc
    51481 tgtctcccaa taagtgggcg cgctaatact atgatatagt gatcggtaat gcagtacagg
    51541 aagatcaaaa ccgctcccat tccagctcac cagggtgggt gtatgtttgt caattccagc
    51601 aaaaaaacgg gttattaatt ctttttcatc agaattttca tcgccaagag accatacttt
    51661 tatttgattt ccatgactga ttaccactga tatagcgcag attttttgta agtaatgagg
    51721 caaaaaatca ttgcctgctt tctcacgcct taaggcaaac atagcgcatg ccgtatcttc
    51781 atctgataag ccatctagtg caaaaagcct tcgccctgac tcaatatctg gaattgtttc
    51841 aatatcaaaa acaagtattg tcataattta ttagtccaat tcggtcttca ttcttatttt
    51901 tgcaaatttg cttgaaatta tcgacaagtc tcggatttgt caaaatcaca attgggatat
    51961 ctgattgatt ctatcatgtg aaggaataga agtaacttta aatggcatag attgtatgca
    52021 gtatatttga tacaatattt tttaaattga taccttatat cgtagcattg atattcaata
    52081 ctgcaggatg agttttttat cattaaatca tgaagattcg atttacttta ctttgtttta
    52141 gcatttttct tacgggctgc gtcagcaaac cgccagcaga tattaataat atttgtaata
    52201 tatttaaaca atatccgaaa tggtatgtgg actcaaagga tgttgagcga cgctggaagg
    52261 ttccggttcc ggtccaaatg gcaattattc atcaagaatc aaaatttaat gctcgagcca
    52321 ggccaccaag aaccaaactg ttatggataa ttccatggac aaggccatcg acagcttacg
    52381 gatatactca ggcgttgcgt tcgacttggg acaattataa acgcaccaat ggatggtttt
    52441 ggtcatcacg cgacgatttt ggagatggtg ttgattttat aggatggtat gctaaccagg
    52501 cgaatattaa ggcaggtatt ccccgaacag atgcttactc actttatttg gcttatcatg
    52561 aagggatagg tggttatcaa aggaaaacct atttgaaaaa accatggttg atttctgtag
    52621 cgcgcaaagt gaaggctaaa tcacaacttt atgcaatgca gctcaattct tgtaaagatt
    52681 cgttgaagtc acgttcctgg tggtaattaa gatggcctaa ttatgttctt gccagtggaa
    52741 ataaagtcag atttggaaca tatatgattt tgtgtgttta taataaccgc cataacacga
    52801 agtcaatctt aagtaaggta tacatttcta tttttgaata gaaaatcggg taaaatcctg
    52861 tttatttaac agaataaagc ttgaaaagaa aaggtgagtg atagtctgct tatgatgtgc
    52921 ctactcaatc cttatagtct taaaaaacct gttttttgag ctgctcattt agttttttaa
    52981 gactatgcat gaccaaaatt atttgtcaat gtgcactaaa taattataaa attttatagt
    53041 gctactatgt tactatattt gaaagtatat aatgattacg aattatttaa ggagttttgg
    53101 atgcgattaa tgcttttagg tggaccggga gccggcaaag gaacccaagc ttcactttta
    53161 attaaccgtt ataaaattcc tcaaatttct accggtgaca tgctgcgtgc tgccatagca
    53221 aaaggtacgc ccttaggatt aagtgcccaa aaaatcatgg aatcaggtgg gttggtgtcc
    53281 gatgatatta ttatcgggtt agtgaaggaa agactaaaaa accctgattg tgatagagga
    53341 ttcctgtttg atggttttcc aagaacattg gtccaggccg aagctttaaa agatgcagaa
    53401 atccatttgg atcatgtgat agaaatagcc gtcgatgacg aagaaataat tgaacgcatc
    53461 agcgggcgac gcattcacca gccttctggt agggtttatc acgttgtgaa tcaaccgccg
    53521 aaaaatccgg gtgtagatga catcacaggt gagcctctga ttcaacgtga tgatgataaa
    53581 gaagaaacca tacgtaagcg cttacaggtt taccatagcc aaacagcgcc attggtgcaa
    53641 tattataagg aatgggctga atctggctct aaagaagctc caaaatttca cactattagt
    53701 ggtacaggta cagtagagca aatatttgac aacatcgtaa cgatactcga aacttaggag
    53761 ttaacatgcc gacccagact attaacagcg ctgaatttga atcattaatt aatgacaatg
    53821 agatagtttt tattgatttc tgggctgagt ggtgtgctcc ctgtaagcaa ttttcaaaag
    53881 tatatgagca agtttccgat caatttccag ctattaaatt tgttaaagtt aacattgaag
    53941 agcaacaaga gttagcagat tttttcgaga tccgatccat ccctcatctt atggttttca
    54001 aagacagtat tgttatttat tctgatgctg gaagcatacc cgagtccact ttaaaagaat
    54061 tggcgcaaca agcacttgat gctgatgtta gtgaaatacg agctcagctt gagcagaagg
    54121 ataagtagat atactagcca gataatccgc aagttctttt ttactggcat tatccatagt
    54181 aaggccgagt ttagtgcggc ggttaagtat gtcatcacaa taattcgccc actcttctaa
    54241 aataagataa tctacttcca cctggtagag tgatgggcca tattttttcc ccattgcttc
    54301 catattgttg cattgcgaaa gaaaaatttc agtataacaa ccataggtat tgaggtagcg
    54361 atttaatagt tcatcatcca gccaatgata ttttgttttg gcgtaattga catattgagt
    54421 aaaactcata tctgctaaag ttgccccagg taagggcgta tgttgtgttt tcgatggctt
    54481 catttgtgga aatacagggg taagttgatc tacaacttct tcagccaact gtctataagt
    54541 ggttattttc cctccaaaaa ttgtaataat gggagctggg gagtggttaa actcatagga
    54601 atagtcacga cttaatgatt tgacttcttt gctatcatca gctaacaaag gccttatgcc
    54661 gctccaggaa tggactacat cttctttagt tattttaaac ttgaagtagg catttaccaa
    54721 ttcaattaag taatcaattt cttcttcgcc aatctctatg tggttaagat ccccagtata
    54781 gtggatatcg gtggttccaa tcatactaaa gccatgatag gggataacaa aaataacccg
    54841 ctgatcatta tgttgcaaga aataggcatg atttccctca aaaagtttgg ggacgataat
    54901 atggctacct ttaaccaggg taatatcttt ttggataggt gtatgagtta attgggcaat
    54961 ggattgcacc catggtcctg tggcattaat gatgctttta gcataagctt tatagctctg
    55021 ccctttggtg ggctggatag ttaaatgcca tatgttattg attacctctg catgggttac
    55081 tttggcatgt ggccttatgc ttgcgccatg atttttggct tgtatggcat taagaatggt
    55141 taagcgagca tcatcggtgg tggcatcata gaaaagaaaa ccgcgattta gctcctcttt
    55201 tagtggcgta aaataattat tcttattatt tctgtggatc gatctggatt tgggtaaatg
    55261 attttttcga ctgagatgat cgtaaaaaaa taagcctaac ctcaataacc atgaggggcg
    55321 catatgtttt aagtagggca aaacaaaaga ttgggaatgg actaaatggg gagccaaatt
    55381 gagaagcgtt tgacgttcct ggagtgcttt tttgactaaa ccaaactcat aatgctctaa
    55441 ataccttaaa ccaccatgaa ttaattttgt gctacttgat gatgttttcg aagccagatc
    55501 gtcctgttcg aagagtatca cagacagacc tcttaatgct gcatctgcag cgcagccgca
    55561 gccattgatg cctccaccaa taattgcaac atcaaaaacc tgatccatgt ttttatctcc
    55621 gaaaaaagat tacactcaat atctataaat atagagggat tttacaataa tgaactatat
    55681 actagctatt gaccagggaa caagtagtac tcgcgctatg atctacaccc ttcaaggtga
    55741 tttaatcgca aatagtcagt atcctcttac tcaatattac cccaattcgg gttgggtaga
    55801 acacgatcct gaggaaatat ggcaaaaaac tctgaatgcg atgagggatg tcgtatctca
    55861 agttcaagga aaagcaattt tatgctgtgg ccttaccaac caacgggaaa ctactctgat
    55921 ttggaacaaa aaaacagggg aatgcctcgc gccagctatt gtgtggcaag atagacgtac
    55981 ggaggagttt tgttattcct tgtctgaata ctcgtcaatg atacaagaga aaaccggatt
    56041 acttcctgat ccctattttt cagcgagcaa gttaaactgg ttactaaaga ataatgctca
    56101 agctcaatca ctaattaacc agggggattt ggcatttggg acaatagaca gttttttaat
    56161 atggcgcatg actaaaggtg caagtcatgc taccgatatc accaacgcat ccagaactat
    56221 gctgtttaat attcatgatg aaaaatggga taagcagttg ctggagcttt tttctatttc
    56281 agaatcggta ttaccagcgg tatactccag tgataatcat tttggggtga tagataaaga
    56341 atggctggga gtagaaatac caattaccgg ggttgcaggt gatcaacaag ccgctttaat
    56401 tggacaaggt tgttttaatg gaggaatggt taaggctact tttggtacgg ggggattttt
    56461 attactcaat acaggtgata aaccagtttt ctcaaagcat aaattgttga ctactattgc
    56521 ctataaaatt aaagaaaaaa cagtatatgg tttggagggt agtatttatc atgccggaac
    56581 tactgttaaa tggttacgcg atgaaatgaa gttgatatct cattctgctg aaactgaagt
    56641 attggctcga agtcttgata gcaataacgg cgtatatctt gtttcatctt ttactggtct
    56701 gggagcacct cattggttat caataacggg aacagccatt ataggtgtat cccgttcaac
    56761 gaatagagca cattttgcca gggctgcctt ggaaggagtt tgttatcaaa cacgtgatgt
    56821 attaacctgt atgagagatg atagtcaatt ggatttgact ttattaagag cggatggagg
    56881 gatggcagta aaccaatggt ttctacaatt tatggctgat cagtgccagt tgacaattca
    56941 aaaacccaaa gacattgaaa cgactgccaa aggggcagca gtattagcgg caataggttc
    57001 tggactattt gattcagttt cttcagtaaa taagatatgg gacgttgaga acgagtttca
    57061 gccattcagg gaaagagagg tagttgaaaa agattttcgc ggctggctag aggcgttgtc
    57121 aatggttaaa gcaaggcatg catagcctag tcttgtatat aaattggtgc gattaaatta
    57181 gattgcagta gctcgtatta tagtttaata caagctactg attaacacat tggatttttt
    57241 attttacgtc acgaattttt tcaccagtat acaattggcg aggtctgcca atgcgtgact
    57301 tggtggctac catttccatc caatgactca tccatccaac agtgcgggcc agagcaaata
    57361 tcactgtgaa catattggtt ggtattccaa tagcacttaa agtcagtcct gagtaaaagt
    57421 ctacattggg gtagagtttt ttctcaatga aatattcatc ttcaagagct atacgttcaa
    57481 gctccattgc caatttgaat aaaggtgcgt ctttggctcc taccgcttct agtacatcat
    57541 aacaggtctt acgcattact ttagctcttg gatcatagct tttatagacc ctgtgcccaa
    57601 accccatcaa gcggaatgga tcatttttat ctttagctcg tttaatgtaa tgatcgatgc
    57661 gactcacatc gcctatttct tttagcatat ttaagcaggc ttcattcgct ccaccatgag
    57721 ctggtcccca taaagcccct ataccggctg aaatgcaagc aaagggatta gctccggtag
    57781 aaccggccag acgtaccgtc gatgtagagg cattttgctc atgatcggca tggagaataa
    57841 aaatagtatc catagccctt accagtacag gatctggcgt agtatcttca gatggaacac
    57901 caaacatcat atggagaaag ttctctgcat aagacatttt atttttagga tacatataag
    57961 gcatgccaat ggagtattta tagctcatcg cagctaaagt aggcatttta gcaaccagtc
    58021 tgattgcgga aataaaacgg tcttgtgcgt tattcaaatc catggattca tgataaaaag
    58081 cagacaaagc ccccacaatt ccaaccatta tagccattgg gtgagcgtca cgtctgaacc
    58141 cattcaaaaa ctggtacatt tgctgatgaa ccatagtatg attgttgatc agactaacga
    58201 aagctttctt ttcttctttg ttaggtagtt ctccattgag taataaataa cttacatcaa
    58261 ggaagtcctt tttttctgcc aattgttcta taggataacc cctatacaac aaaatgcctt
    58321 tatccccatc aatataggtg atttttgatt cacaggaggc agttgataaa aaaccttgat
    58381 caaaggtaaa gacgccatgt gaaccaagct gggtaatatc aatcacatca ttacctaggg
    58441 tgggagtata gattggtaaa tcaatcgtct cttgcccatc gatgctgagt ttcgctgtct
    58501 tttgagtcat tgcttctcct gattatttac atgcttaaag cattcatact tgcattagtg
    58561 cgcggaacta tataaaaatg acaacataca gtcaaattaa acataatttt ggtcattaaa
    58621 aatgctagtc ccatatccaa tattaattaa atattcaaat aatttagatc atttgcaacc
    58681 gtatacattg attatatatt atggtttaca aatttgagga ataatagaaa aaaaaatgat
    58741 aactccagcc gaattaccta tcaatcatcg tggtgcggta tatcatcttg atttaagaac
    58801 agaggaaata gctgatctca tcattacagt gggagaccct gggcgagtaa gcctggttag
    58861 tcaacatttt gataaaattg aagtgcaacg ctcccaccga gaatttgtta ctcatacagg
    58921 gtatctggga actaatagaa ttacagttct ctcaactgga attggcatgc caaacataga
    58981 tatcgtgatg aatgaactgg atgctttggc aaacatcgat ttatctacac gaacagtgaa
    59041 taccaccaaa aaaagtttaa caataattcg attaggcacg acaggagctc tgactgagaa
    59101 ttgtttgccc ggagatatag ccattagtcg atatgcaatt ggttttgata gtttgattga
    59161 ttattatcaa tatcagccac tgggagtgct tgaaacaatc aaggaggaat tatctcaata
    59221 tttaaaggga gagagtggtc cgttttatat ttctgaatct tgctctcaat tagctgaaca
    59281 gttcagctcc ttgggacata caggaattac tgctacttgt ggtggttttt atgggccgca
    59341 aggtcgccag ttaagattgc cgcttcatta ccccaacctt attagacagt taggaacatt
    59401 ttgcactcag aattattcag taattaatct tgagatggaa actgctgcta tctatgctct
    59461 tggcagtatg cttggacata aatgcctatc cttaagtgtg gtgattaata acaggataca
    59521 tggaagtttt gcagcagatg tgaaatcttg cctggattcc ctgatcacaa aatcactcgc
    59581 gaacattaaa aatatccttg agctatccga agcttaaggt gatttaatta agacgcccag
    59641 tgtggtatcc ttttgcatta aattaatctt aatttactgt ctaaaggata catccaaatt
    59701 atggtatacc tagccaaaga agtcttacca gtcaacatag aagacgaatt gaagcaatcc
    59761 tatttggatt atgcgatgag tgtcattgtg ggtcgagcat tacctgatgt acgtgatggt
    59821 ttaaagccag tacacaggcg agttcttttt gcgatgagtg agttaagtaa tgattggaat
    59881 aagccgtata aaaaatctgc tcgtgtagta ggggatgtca ttggtaaata tcatcctcat
    59941 ggggatacag ctgtttatga cactattgtt cgtatggctc agcccttttc catgcgttat
    60001 atgctgattg atgggcaggg taattttggc tctgtagatg gagatgctcc agctgccatg
    60061 cgttacactg aagtaagaat gtccaaagtg gcgcatgctt tactggctga tttggataag
    60121 gaaaccgttg attttagtcc taactatgat gaaacagaat ttgctccagt ggtattgcca
    60181 tcgagaattc ccaatttact agttaatggc tcttccggta ttgcggtagg gatggctact
    60241 aatattccac cacataatct taccgaagta atcaatgcat gtattgcttt agtggatgaa
    60301 cctgacacga gtcttgaaga tttaatggaa attattcctg gccctgattt tcctacagcc
    60361 gcaattatta atggtcgtgc tggaattatt gaaggttatc gtactggaaa agggcgggtt
    60421 gttatcaggg cacgcacaga aattgaaacg gatgaaagtt caggccgtca gtcaattatt
    60481 attcaggaat taccctatca ggtgaataaa gcgcgtttga tcgagcgtat tgctgaattg
    60541 gtaagggaca agaaaatcga aggaatttcc ggcttgagag atgagtcaga caagcaagga
    60601 atgagagtag tcattgaatt aaaacgcaat gaagtagcag atgtggtatt gaataacctg
    60661 ttcgctcata ctcaaatgca aaatgtattc ggaattaata tggttgctct ggtggatggc
    60721 caaccgcgta ctttgaattt gaagcaaata ctggaatatt ttataaaaca tcgaagagag
    60781 gttgttacca gacgcacaat atttgaattg aaaaaagcca gaagtcgagc ccatttattg
    60841 gagggcttgg gaatcgcctt agctaatatc gatgaaatga tcgcattaat taagcaatct
    60901 cctactcctc aagacgcaaa aagtgcttta ttaagcaaaa tatggcaacc tgggttggta
    60961 aaggccatgt tggaaaaggc tggctcaaac gcgtcaaggc ctgatgattt aactgaagag
    61021 tatggtttgc atgagagcgg atataaatta tctgaagcgc aggcacaagc aatacttgaa
    61081 ttaagattac ataggctaac agctcttgaa caagataaaa taattaatga atttgaagag
    61141 ctattgaatt taattaaaga attacttgat atattggctt cacctgaaag acttatgcaa
    61201 gttattcgtg atgaattgat agagattaaa tcccaatttg gtgatgagag acgaacggaa
    61261 ataacagcct cacaagaaga tttgactatt gaagatttga ttaccgaaga agatgttgtg
    61321 gtcactttat ctcatcaagg ttatgtaaaa tatcagccca ttacagccta tcaggctcag
    61381 cgccgaggtg gcaaaggcaa atcggcaact catgtcaagg atgaggattt tgttgagcgt
    61441 ttggttattg cgagtactca tgacacctta ttatgtttct ccaatcatgg caaattatat
    61501 tggctaaaag catatcaatt gcctcaagca agccgcgctt cgagaggaag gcctataatc
    61561 aatattcttc ctttggcaga aggcgaagag attaatgcta tgctccctgt tcgtgagtat
    61621 aaagacggca gctacgtgtt tatggcaacc aagaagggaa cagttaaaaa agtgccttta
    61681 aacgcattta gcaggccgcg ttccaatggt attattgctg tggacctgga agaagatgac
    61741 agtctcgttg gagttgatat tactgatggt gctcgcgata ttatgttgtt tactgatgca
    61801 ggcaaagtaa tccgctttga tgaaagtaaa gtgcgaccta tggggcgcac ggctcgagga
    61861 gttcgtggaa ttagggtaga aaaggatcaa gccgttaaat ctcttgtggt agtcgatcca
    61921 agtggaggca ccattttgac ggcaactgaa aacggctacg gcaagagaac tcatattgat
    61981 gaatatcgag tgtcaggacg gggcgggcaa ggtgttattt ccattcaggt tactgaacga
    62041 aatggtaaag tggtccgctc cttgcaggtt accgataatg acgaagccat gttaattaca
    62101 gataaaggga ctttggttcg ttttaaagtt aatgaattat ctgtgatagg ccgaaataca
    62161 caaggagttc gtctgattaa cgtcagttct ggtgaggcag ttgttggaat gcaaaaaatt
    62221 gtagatcttg gagaagaatt agaagaggca gaagattcat cccttaatgc tgaagacagc
    62281 agtgatgaat agcagggttt tcaattttgg agctggccct gccatgctgc ctgaggagat
    62341 attaaaagag gctcaagagg aatttcttaa ttggcgtaat accggcatgt ctatacttga
    62401 aatagggcat agaacaccgg aaatcattag tcttttaagt actgcagagc aatcgttaag
    62461 agagctgtta aatatcccaa aaaattatca tgtattattt ttgggggggg cagctcgggc
    62521 acaatttgcc atgattccta tgaatttatt acggccagga gatgatgcgg cttattttat
    62581 tacgggaatc tggtctaaga tggcttatca tgaagcgaat cttctaaaaa aagcatatta
    62641 ccttagcagt gaagagaaag aaggatttgt atcaattcct gactatcaaa agtgggagtt
    62701 aaaaagtaat acagcctatg tctattatac cccaaatgaa acaattaatg gagtcagatt
    62761 tccttatgtt cctaaaacag aaggtgttcc tctagttgcg gatatgactt cttgcttgct
    62821 gagcgagccg ataaatatta ggcaatatgg tttgattttt gctggtgcgc agaagaacat
    62881 tgccaatgca ggtttaactg tagttatcat tcatgaggaa ttactacata atcaacctga
    62941 gccagtgatt cctaccatgt tgaattataa aaatcatgct gatcatcgct ccttatatgc
    63001 tacaccccca gtatttaatt gttatttggc aagtaaaatg ttcgaatgga taaaaaaaca
    63061 agggggtata gaagaattat ttcagaggaa ttgtttgaaa gctgcgaagc tgtatcaata
    63121 tctcgattca actgattttt atttaactcc agtatctaaa gaagctcgct ctataatgaa
    63181 tgtttgcttt tccctgtact atccggattt ggaacaaaaa tttctcgata tggctaatga
    63241 gcgtgggtta aaggctttaa aaggccatcg tttcactgga ggattgcgcg ccagtttgta
    63301 caatgccatg cctatggctg gagttgatgc tctcattgag tttatgtctg aatttgcaaa
    63361 agaaaatggt taataagcat gttgaatttt ataagtaaac cagtaggctg ccttaagggc
    63421 gaaatcacag tacctggaga taaatccatt tctcatcgtt ctattatttt tggcgcgatt
    63481 gctattggaa cttcagtaat cgatggcttt ttagatggtg aagattgcat agccaccctt
    63541 aaagcattcc aatcgatggg tgttaggata gaggggcctg ataagcaacg tgtcattatt
    63601 catggggttg gtaaatatgg tttaaaacaa cctcaaaata ttattgattg tggcaactca
    63661 ggcaccagta tgcgcttgct ggctggattg cttgcagcac aacaatttga tagccagtta
    63721 acgggagacg aaagtttact taaacgccca atgttaagaa ttagtagacc cttaagtcaa
    63781 atgggcgcag acgttactac tcaggatggg aagccgccta tagtaatcaa aggcggaaaa
    63841 aaattaaatg gaatacatta tgttatgccg gaagccagtg ctcaggtaaa gtcgtgtttg
    63901 ttgttagctg gtatgtatgc tgaaggacaa acaaaaataa ctgaaaatgc agtcagtcgg
    63961 gatcatactg aaagaatgtt aagaacattt tcttatccag ttcagataca agatggtgct
    64021 atcataattg attgtaatgg cgagtgccat ggaactcgtc tgaatatccc gggagacatt
    64081 tcatctgctg ctttttttat tgttgcagct tctattactc caggttccga tgttttaatt
    64141 cgtaatgtag gtattaatcc tacacgaact ggcattattc atattttaac tgaaatggga
    64201 gcggacataa gagttttgaa tcaacgtgcc tatggagagg aaccagttgc tgatttgcat
    64261 atcagatatt cccaattaaa aggaatcgat attcctgcct ctatggtgcc tttggcgatt
    64321 gatgaattcc cggttatttt tattgcagct gcttgcgcac aagggaaaac cactcttcat
    64381 ggagctaaag agctgcgctt gaaggaaagt gatcgtattg gcgcgatggt ggatggctta
    64441 aaccaattgg gagttcatgc tgagggtttt gatgatggaa tattgataga gggtggaagt
    64501 atacagggtg gtgaggttaa tagtcgagga gatcaccgta ttgccatgtc atttgccatt
    64561 gctggcgctg tcgccagtgc tcctgtaacg attaaaaact gcgccaatgt tgcaacttca
    64621 tttccatctt ttgttactac cgctaacatg cttcattttc agatagagga atacagctga
    64681 tggtttttaa taatgaggtt cctgtcatta cgttggatgg tccaagtgga acaggaaagg
    64741 ggacaatttg tcatttggta gctaagagac tgcattggaa tatgcttgac agtggtgcta
    64801 tttatcgtgt tctagcttat gctgctcgga aaaatagcat cgaaccgaat gatattaaaa
    64861 aattgactgc tttggcccgt tccctgaatt tgcgttttga atcatctgcg gataatgaaa
    64921 caaaagtaat acttgatgat gaaaatgtca gccagcaaat ccgcagtgag caatgtggtc
    64981 aggatgcctc acaaattgca gtgattcctg aagtaagagc agcactgctt gaacgccaga
    65041 gaaactttgc ccaatttcct ggattggtga ccgatggacg cgatatgggg acggtggttt
    65101 ttccaaatgc tgttttgaag gtttatttat atgcaagtgc agaagaaaga gcaaatagac
    65161 gatatttaca gttgcaagat aatggaatta atgttagcct cgcccaggtt gttgaagaac
    65221 tggctaaacg ggatgcaagg gatacagctc gtacgcatgc gccattgaaa ccagctgaag
    65281 atgctgtctt gatcgataca accggactaa caattgtaca agtgttcaat attgtattaa
    65341 aattaattga tgaacgtttg aataatttgt agttttgaat tggggggaag gtgagtggaa
    65401 ttcattcttc cattttttta ctaaagagtt tattaacatg tctgaaagtt tcaaagaatt
    65461 atttgagcaa agtattgccg gagctcaatt ttatcctggt gccattatta ctgctaaagt
    65521 catcgatatt gatgatgatt ttgttactct caatgctgga ctaaagtcag aaggtattgt
    65581 tgccgtagaa gaattctacg ataaaaatgg cgaattggaa gttaaagtag gcgatactgt
    65641 cgaagtggcc ctggattcag tagaagatgg tcacggcgaa acgctccttt caagagaaaa
    65701 agcaaaacgc caggaagcat ggcgcaagtt atcgaaatgc cacgaaaata atgaaacagt
    65761 cactggtttg atttctggaa aagtcaaagg tggttttact gtggaaattg gctcaatcag
    65821 agcattttta ccgggttccc ttgttgatgt taggcctgta agagatccat cttacctcga
    65881 aggtaaagaa cttgaattta aagtcatcaa gatggatttg aaacgcaata atattgttgt
    65941 ttctcgacga gctgtagttg aagaagaaag tagcgctgac agacaagcac tactggagtc
    66001 tttacatgat ggtcaagagc ttcacggtat tgttaaaaac cttaccgact atggtgcttt
    66061 cattgatctt ggcggcatag atggtttgtt acatatcact gacatttcct ggaagcgagt
    66121 caagcatcca agtgaagtat tatcagtagg tcaggatgtt aaagtcaaag tactaagctt
    66181 tgatagtgaa agaaaccgcg tttccttggg tatgaagcag ttagggaatg atccttgggt
    66241 tgatttggtt gaacgttacc caataggcaa gagattgcaa ggtaaggtaa ccaacatcac
    66301 cgattatggt tgctttgtcg aaattgagga aggtgtagaa ggtttagtcc acatgtcaga
    66361 aatggattgg actaataaaa atgttcaccc aagcaaagta gtgtctctgg gagatgtagt
    66421 agacgttatg gttcttgaaa ttgatgaaga acgacgacgt atttctttag gcatgaaaca
    66481 gtgtgtgggt aatccatggc agcaattcgc atctactcat aataaaggcg aaaaagtaaa
    66541 aggcaagatc cgttctatta ctgactttgg aatctttata ggtcttgatg gtgatattga
    66601 tggactagtt catttatctg atatttcctg gactgttcct ggtgaagaag cggtaaaaca
    66661 atttaaaaag ggtcaagagt tagaagctgt tattttagca atcgatccag agagagagcg
    66721 tatttctcta ggattgaaac aattggaagg cgacagtttt gccagttttg ctgaaactta
    66781 cacgaaagga tctattgtta aaggtacagt tacagctgta gaacctaaaa ctgtaacagt
    66841 tgcgttagca gaagatgttt ctggcacaat tcgtgtgagt gaattatccg atgaacgtgt
    66901 tgatgatgct accactatcg taaaagttgg tgatgaagtt gaagcaaaaa tcactaatat
    66961 tgatagaaaa aatcgcacca tttcactttc cgtcaaggca aaagatgccc aggatgaagc
    67021 ggatgcaatt aagaagtact ccagaacaga ggcagcatca actactctgg gtgatttatt
    67081 gaaagaaaaa atggctagca aagaaggtga ataacctttt taagtcaaaa ctaaaaaagc
    67141 gtagaactat ctacgctttt ttttattttg agaaaaagat tatggtatga tggacgtttt
    67201 agcttaagga ttaaaggaaa tattcaatgc gcatattaat gctggtaact tatctgctgc
    67261 ttattattat tggtgttagt tttgcggctc taaatgcctc gtcggttcaa gttaatttct
    67321 attttaaaac ggtgtccatg cctatctctg ttcttatgac tatcatgctc ggagttggtg
    67381 tttttatagg ttttgttctc tttattacac gatattggcg tttaaaaatt gaatgtcgta
    67441 aaatgaaaaa ccaattaaaa ctaactgaaa aagaaattaa gaatttacgt tcaataccct
    67501 tacaagatca acattaattt tattattagt ggaaactcga tatgcagaga ggaaaattaa
    67561 tagatgatta atttatggcc actgttgtta cctgctgccg catggtctgg ctggtggctg
    67621 gctaaccgca gtaattccaa taaagatgct catgtccaca atcgcttatc gcgagaatat
    67681 gtagttggac ttaattattt attaaatgaa caatctgata aagcggttga cgtatttatc
    67741 aagcttctag aagttgatag cgatacagta gaaacccatc ttgcactggg tagcttgttc
    67801 aggcgtagag gggaagtaga cagagctatt cgtattcatc aaaatttaat cgccaggcct
    67861 caattaagta ttcaacaacg aaaggaagcc ctaatggctt tagggcaaga ttatatgagt
    67921 gctggagtgt ttgaccgagc tgaacgtata ttccttgaag ttgttgagtt gggcgggagt
    67981 agagatacaa gcagtcttca aggattatta gcaatttacc aacaagaaaa agcttgggaa
    68041 aaagctttag atattattaa gaaactggaa atttctaccg gagccagttt ccacaatcaa
    68101 gcagctcatt attactgtga aatggctagt caagcgctta aaatcaatgc aatggataga
    68161 gcaatttatt gtataaagca agcaatgaat gttgatccgg aatccgtaag agccagtttg
    68221 atgaatgcta caattgaaat aaaagaaggc cgctataaac aggctattcg atctttaaaa
    68281 agagtgccgc agcaggatgc agagttttta accgaaatca ttgaaccatt agttctttgt
    68341 cataaagagc tggactccat gccggattgt attcactatc ttgagcaaac tttggaaaaa
    68401 catccgagag cctctgtaat tttcgttata gctgattatt tgcgacaaaa taagggtatg
    68461 gatattgcaa ttgactttgt ggctgataat ttaagcaaac atccatcgat aagaggtttg
    68521 aatcgtttga tttattggca cttggagtct gctcatggga aagtacgtga aaaactgcaa
    68581 atgctttatg atattactag caagttttta gataataagc ccatatatcg atgtgggcat
    68641 tgtggttttg gtggtaaaca tttacattgg cattgcccaa gttgtaaaca gtggggtaga
    68701 atgaagccta tccatggttt ggaaggggat taacctgaga catactctca gaaagttaat
    68761 gaacagttgc atattagctt agtatcgtaa ttcaaatttg ttattgcaaa ttaagtaaaa
    68821 caattcaaat aggaaattta atttaatttg atttgttttg tcttctcgtt tgccaaggca
    68881 gtttacgttc aaatcagttt attgcaactt tagatttgta ttaagattat tccttaactt
    68941 catggtattg atcttaggta tagctaaaca atttatatac taattatgag attataatgc
    69001 aatttatcga cttaaaaaca caatattctt taattgagaa tgatatttta aacagcatta
    69061 aaagggtatt aaatcatggc caatatatta tggggcctga aattgcagaa cttgaaaaag
    69121 agttagccac ttttgttgga gtgaagcatg cgattgtgaa ttcaagtggt acagatgctt
    69181 tattaatggc attgatggct ctggatattc agcctgggga tgaagtaatt acaagcccat
    69241 ttagtttctt cgcaacagca gaagtgatta gcctttgtca tgcaacaccc gtctttgtag
    69301 atatagatcc tttaacttat aatttggatc ctgagcagtt agagagagca atcactaaga
    69361 aaaccaaggc cattatgcca gttgatttat atgggcaatg cgcagattac gatgtgatta
    69421 atgccattgc aactcaatac ggtattcctg ttattgaaga tgctgcgcag agttttggag
    69481 ctacttataa aggcaaatac tcttgttccc tggctactat tggctgtact agttttttcc
    69541 cgtcaaaacc tttaggtggt tatggtgatt ctggagcttg ttttactaat gatgatgttt
    69601 tagcccaaaa attaattgaa attagaattc atggacaaaa tgcccgctat tgccatcatc
    69661 gggtagggat taacggtcga atggacacta tccaagcagc aatactactt cagaagctga
    69721 aaatattttc taatgaaatt ctattgcgac aaaaagtagc aaaacgttat gatcaaatgc
    69781 tttctgagtt agtgaaaact ccttatgtgc atgaatgcaa tgcgagtgtg tatgctcagt
    69841 ataccattga ggttgagaat agagatgtat ttgcgaaatc aatgcaagcc tcaggtatac
    69901 ctacagctgt tcattaccca attgctatgc atcaacagca ggcacttggt tacttaaatt
    69961 acaaacttgg agattttcca aactctgaaa aagcaagtca acatgtaatt agcctgccta
    70021 tgcaccctta tttacaagaa gatgagcaat tgaaaatagt tgcagcagtt agaaatgctt
    70081 tacttgtgat gaatgaagag gccatggtat aaatgacacc taaattaatc gttgcgttgg
    70141 attttgataa tcaagataac gctatacagt tggttgaaaa acttgatccg aatcattgcg
    70201 cacttaaggt tgggagtgag ctttttactc tattagggcc tcagtttgta aaagcattgg
    70261 taagaagaga gtttaaagta tttttagatt taaaatttca cgatatccct aatacagttg
    70321 ctaaagcatg ccactccgct gcagaattag gggtatggat gataaatgtt catgccatcg
    70381 gcggcttaaa aatgttacaa gctgccaggg aatctttaaa aacgtatggt aaagacaggc
    70441 ctttgttgat agcagtgact gtgctgacca gttttgaaga aggggagctg gccagtgttg
    70501 gtattagtaa cacattgcct gagcaagcaa cccatctggc tatgcttgcc agagaggctg
    70561 ggcttgatgg tgtggtaagc tctgcacatg aagttaaaat tataaaacag aagtgtggtg
    70621 agaattttat taccgttact cctggcataa gactgccaaa taatctaaaa gatgatcaat
    70681 cgcgagtaat gactccacaa caagccatca gagaaggaag tgattttctg gttattggaa
    70741 gaccaattac gcaagcgagc aatccttatg aggttgtctc agcattatta cgtgatcttt
    70801 gatggcgcat acttttaaaa aattatacat atggtataat ttttttcatt tttgaattag
    70861 ttgtaccatt cctattttat tccatagaga tttatgataa ttgttataaa gatgattaat
    70921 aaatatttaa aaataaccca tattatttca gatatttatt ctatggacta cccagacgta
    70981 aggatttact gatgacccca cagcacgtca taagtcaact gctggaatct gataccaaat
    71041 accctcagaa tttagattta ttaaaaaaaa taatcatatc agcttatctg ggacgcctca
    71101 gaattaatgg attacctcca gataacaaga ttgcacttgg aaattattta tttgatgatg
    71161 aacgtatgat ttttgatttc actcgattga gtgattcaaa aagagcttta tttattgaat
    71221 ggttgcttga gcctcatcaa aaagataaag agcatggttt tttaagtggc gtttacgtga
    71281 atgaatatcg aggtttcact gcggaggttg ctttaagttg gtggggtatc ttaaaaaact
    71341 ggttatttaa taataaatct gagcattgga aaataacgga tttgggactt tctgtcaatt
    71401 atcagctgac tggtattgaa atgtgtcatg gaaagcaggg tatcttaatt ggatttaacc
    71461 agtttttagt accaccttca ggtacaaaat acagagatcc gaatgatagc caaagtgagc
    71521 cattaggtaa tgttaaaaga gtattcatta ctgataaact ggttgatgaa cttgttgggc
    71581 tcaattataa aaaacttaat tatgagtctg tctgtaaaag ccctcatcct caatctattg
    71641 atgtaactga tccagaagct cgtcataaag acatgtatga ctacagaaaa atgcaacgtt
    71701 tcaatggtgt gaggccttgg tatatcagac tttggagatg gatatctaaa ctattttttg
    71761 gcgatacata cgacgctaaa aaaacaagca cttcagacga caatttggaa ttactttata
    71821 aaactaaaac tgtcaaaatt tatcagcgct ccaaatcaaa agagatcttg gtcacagaga
    71881 agcgacctga tattactaat ttggtttttt gtggaggtgg ggcaaaaatt tttgctcacg
    71941 tgggggtttg gaaggctctc aatgaagcag gaatgaagcc aaccaaattt gcaggaagct
    72001 ctgccggtgc aatcatgtct ttgatgtgct acctgggtta tactgctgat gaaattagtg
    72061 aacttttcaa gcatttcaaa caagagcatt tagttcaatt tgatatagac agaaatggat
    72121 tatctgatac tcattcctta aagaccgcgc tggattatgc tatcgcaaat aaagtaaggc
    72181 aaatagtaaa taaatataaa attccatatc ctaagggaaa aataactttt agtactttag
    72241 cgtctctaag agagcaattt ccagattgtg gaataggcaa agaattaatt gttacagcaa
    72301 caaataaacg ctcaggaaaa actatttatt tatcagcaaa tcgttaccca ttattggagg
    72361 tcagtgaagc agttaaaatt tctgcgagct ttccagtttt gtatagagat actctgctgg
    72421 atggtgaaga gcataatgac ggtggaatat taagtaattt tccaaccgaa gttttttttg
    72481 atgaccactc aacgctgttg gagtctgaat ttggtaataa tttaaaagta ttggcagttc
    72541 aatttgataa t
//
LOCUS       DACTJE010000002        55030 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.002, whole genome shotgun sequence.
ACCESSION   DACTJE010000002 DACTJE010000000
VERSION     DACTJE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 55030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55030
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.002"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..912)
                     /gene="cegC2"
                     /locus_tag="I9760_000071"
     CDS             complement(<1..912)
                     /gene="cegC2"
                     /locus_tag="I9760_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC2"
                     /protein_id="HAT5041874.1"
                     /translation="MTPLFPTQGPITIRQGIGGSCYLLSSLDCILNLGADGEQLIKSL
                     FTQTEDGKVIVRIKRHEALKDNLQKNKMTGKYTHYVDELNNEDVFEISPERLKEIDNQ
                     YGGVKSNSLAIKILERLVSYYYAGDWSNTDPLASVVAHDIPDRIAGFTSTAFLGKFFG
                     IQAEDIPYSKLDDIIKLKLMNPDEPVYISMSYGKVDSFGKFHGRHALRIDKIIPKGSG
                     NYDFVLINPHDNSKTETYKLDDLNKRNCRFCLFNTSIHRASLTKKLLTLSNEEGRYIF
                     SNSGLQKRLISLEEMHLLTDNKIISSCI"
     gene            complement(1026..1628)
                     /locus_tag="I9760_000072"
     CDS             complement(1026..1628)
                     /locus_tag="I9760_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAT5041875.1"
                     /translation="MPINLYSKAVFLKSAARVNQLPEDSGYEVAFAGRSNAGKSSALN
                     CLTNNKNLARTSKTPGRTQLINLFSLDEQRRLVDLPGYGYAKVAMEVKLEWQKNLAHY
                     LEARQCLRGLILLMDVRHPLKDLDQILVNWALHRELPVHILLTKADKLSRSEVKNAVL
                     KVRQYYELAEHLVSVQAFSSVKKDGVEELISLLDRWYEWN"
     gene            1703..2305
                     /locus_tag="I9760_000073"
     CDS             1703..2305
                     /locus_tag="I9760_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HAT5041876.1"
                     /translation="MKKLALVLILCSSSVIFAQENPQEAGQNKSTVCTACHGPQGIST
                     NPEWPNLAGQHEKYFVKQLKDIKEGKSRSAPTMTAIVANMNEQDMDDLAAYYAKMPVA
                     EGSTPEKYLKRGEQLYRGGDLNKHIAACIACHGPKGSGNAQAGFPLLSGQHAAYTIMQ
                     LQAFKDGKRTNDLNQIMQDISSKMSPEDMEAVAYYIQGLY"
     gene            2316..2930
                     /locus_tag="I9760_000074"
     CDS             2316..2930
                     /locus_tag="I9760_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HAT5041877.1"
                     /translation="MFKKLIALLFLMPMTALATQFIEGKDYQTVASAQLSTNKDKTPL
                     ITEFFSYGCPWCYKIDAPLNDWATRMGKGAHLERVPVVFKPNWDLYAKAYYTAKTLAM
                     SDKMNPILFKAIQEDKNPLATKQSMVDFFVAHGVDREIAKSAFENSPTIDMRVNSGMS
                     LMAHYQINAVPAFVVNNKYKTDLQMAGSEERLFEILNYLVRKSA"
     gene            complement(3007..4305)
                     /locus_tag="I9760_000075"
     CDS             complement(3007..4305)
                     /locus_tag="I9760_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HAT5041878.1"
                     /translation="MPETIINIENLSKSFKKAPSQHLLVLEDVNFKLQEGEIVALLGK
                     SGSGKSTLLRIIAGLIAPSSGTVTYRGKPVTRPVEGIAMVFQSFALMPWLTVLENVEL
                     GLEAQGISREERRHRAIEAIDIIGLDGFESAFPKELSGGMRQRVGFARALVINPDVLL
                     MDEPFSALDVLTAENLKSDLLELWKEKKTNTNGILLVTHNIEEAATLADRIVIFGNDP
                     GYIRAELPVTLPQPRDPESPEYLALVDKIYTLMTTGPKEKAKRAQRERQIGLGYRLPD
                     VEPSELSGLIETMKSFEERIDLPELADELMMNIDDLFPILETLEILGFAKVSAGDIQL
                     SELGKQFSEADLQERKQLFAQRLLEKVPLARYIRRVLDEKAGHRVSEERFLSKLEDYL
                     SEKEADRVLKTMIDWGRYAEIFAYDFNTGILSLENPGKGA"
     gene            complement(4313..6052)
                     /locus_tag="I9760_000076"
     CDS             complement(4313..6052)
                     /locus_tag="I9760_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAT5041879.1"
                     /translation="MGNSRFYFANPDKFSRFINRWDLLLLILTFSVLFFLGWAGSQMA
                     TPYQLGDQIPISLEPSNLPFYALRTVLRMFIALIFSILFTFIVGALAAKNRRAEQIVI
                     PAIDILQSIPVLSFLSITVTGFIHLFPNSLLGPECASIFAIFSAQVWNMTFGFYQSLK
                     TVPHDLIEVSAMFRLSAWQRFWKVEVPFSMSGLLWNMMVSMSASWFFVVLSEAISVAH
                     QNIRLPGVGSYIALAIAQRDLHAVGYAILTMVIVIFLYDQILFRPLIAWSEKFKMEQS
                     PDESEYQSWLIDLIRSSRLMKRVTEWLAVFTNSFVNARWLRIGEVKAVKEIDFKRQKR
                     LDRLWTALVLIAVITGGWFLLKFILAELKVSDIFHVFLLGAATGSRVIILILLSSMLW
                     IPVGVWIGLRPRLAQKIQPIIQFVAAFPANLFYPLFVIAIVKFNLSVEIWVTPLMILG
                     TQWYILFNVIAGASSIPRDLYLAADNFGLKGWIWWKRLALPGIFPFYITGAITAAGGA
                     WNASIVAEYVSWGNITLKATGLGEYIQASTTAGDFPQIALGTAMMCVYVLAFNHLIWR
                     PLYRLAEERFNFN"
     gene            6549..7079
                     /locus_tag="I9760_000077"
     CDS             6549..7079
                     /locus_tag="I9760_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmL"
                     /protein_id="HAT5041880.1"
                     /translation="MNKTITIALSLLLSIASKLTYAGPDRAQLAVWANEAIIATYTFD
                     YKNYMQQQKEIAKYFSADGWIAYSKALNQSKLPEVVQKNAYFVNAVATEPPKLITLDP
                     THWQAIMPILVVYKNPQYEQKQNLKVVLGFTVASPGQGVRGFSVTSLQSTPISPPCQC
                     KIEETPGNTKQGDAKQ"
     gene            complement(7054..7521)
                     /locus_tag="I9760_000078"
     CDS             complement(7054..7521)
                     /locus_tag="I9760_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HAT5041881.1"
                     /translation="MVWPIIWRWILDLGWLCFLLILFWYFWKKRRDLVEARSWLKAKG
                     HVTRCEWTRVGHSVWPRIEYIYEVYEKDLTGEYLFLDTTFNTPNSKYSRSIAYKVAIA
                     YRDNSEIDVYYNPNRPEQSALDVTIPKKLTFILILISSLILLHIGIIVWRLLV"
     gene            7656..8738
                     /gene="gcvT"
                     /locus_tag="I9760_000079"
     CDS             7656..8738
                     /gene="gcvT"
                     /locus_tag="I9760_000079"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAT5041882.1"
                     /translation="MTAKTPLHTTHLACGAKMVDFHGWDMPLHYGSQLNEHHAVRNDA
                     GMFDVSHMTIVDILGAGGRQFLRKLLTNDVDQITHNGKALYSCMCNEHGGIIDDLIVY
                     QRASDNYRVVLNSATRQNDVAWIRAKSEGFAVGLQERRELSMLAVQGPNAIAKTLSIL
                     APAHVDAVSTLTSFECVDVDHWFFARTGYTGEDGLEIIVPNEFVTQLWNDLLHAGVTP
                     CGLGARDTLRLEAGMLLYGQDMDETTTPLESGLAWTVKWEPEDRGFIGMGALVSQKQQ
                     GIKRKMVGLTLLDKGIMRHGQKVIIEGCPDGIITSGSYSPTLQQSIALARVPMETGEQ
                     VLVDIRGKLIPAKVGKPRFIKQGKPV"
     gene            8772..9149
                     /gene="gcvH"
                     /locus_tag="I9760_000080"
     CDS             8772..9149
                     /gene="gcvH"
                     /locus_tag="I9760_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAT5041883.1"
                     /translation="MNDLKFTTTHEWLREDEEEVTVGITDHAQELLGDMVFVELPEIG
                     DEVSAGQELGVVESVKAASDFYAPISGVVTAVNEAVGKNPALVNHDPYHEGWLVKLKP
                     SHPDEIKSLLSDEQYQNEIAEEN"
     gene            9152..10522
                     /locus_tag="I9760_000081"
     CDS             9152..10522
                     /locus_tag="I9760_000081"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HAT5041884.1"
                     /translation="MPYIPHTPDDTKEMLEAIGAQDIQDLFDEIPASLQYAGFQNIPA
                     GINEMEMLKEAQNQAQKNRNGICFIGAGCYEHHIPAAVWDIASRGEFLTAYTPYQAEA
                     SQGTLQLLYEYQTMICELTGMEVSNASMYDGATALAEAVLMAVRLNKHSKTNRVLIAG
                     TVHPFYRETIETIVRNQHIEVITLPFDEQQGITNLGSLNQYTGEDITALVIAQPNFFG
                     CLEQVDKMTSWAHHNKTISVACVNPTSLALLKPPGSWGEHGVDIVCGEGQPLGSPMAS
                     GGPYFGFLSTRMAHVRQMPGRIIGRTVDKDGKTGFSLTLQAREQHIRRAKATSNICTN
                     QGLLVTAATIYMSLLGPEGLSQVATQCHQNTHELITALTQIEGVELAFKAPFFHEALI
                     KLNQPVQSVLQQLADAGIAGGYAPEQHYPQLANTLLVCATEVRTAEDIAKYAKTLKTI
                     MSKRGA"
     gene            10522..10830
                     /locus_tag="I9760_000082"
     CDS             10522..10830
                     /locus_tag="I9760_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase"
                     /protein_id="HAT5041885.1"
                     /translation="MFIVQLTYLVPINEVNKYLQAHREFLDYHYKQGLLVASGPMKPP
                     TGGIIIAATNDRAHLESVFKQDPYYLAEIAEYQFIEFTPVKHRNELKELIQKTEGKLC
                     "
     gene            10824..12278
                     /locus_tag="I9760_000083"
     CDS             10824..12278
                     /locus_tag="I9760_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase subunit 2"
                     /protein_id="HAT5041886.1"
                     /translation="MLIFELSKTGRQAKAQIPRAVSKNYSIPEEFQRKSPPRLPACSE
                     LQVVRHFTCLSQKNFSIDTNFYPLGSCTMKYNPRGVHKAASLPGFINRHPLAMDNESQ
                     GFLETLYKLQNYISEITGMPGVSLTPMAGSQGEFAGVAMIKAYHQSRGDTARDEILIP
                     DAAHGTNPASAVMCGFKVVEIATAPDGDIDLDELKRKVGPRTAGIMLTNPSTLGLFMR
                     QIKEIASLVHQAGGLLYYDGANLNAILGKVRPGDMGFDVMHLNLHKTFATPHGGGGPG
                     AGPVAVGKRLIPYMPLPVVKKTDSGYHWATRQDYPQSIGRLSCFMGNAGILLRAYFYM
                     LVLGKEGLLRVSEFATLNANYLLKELTKVGYTAAYPDRRASHEFILTLNSEKKNYDVT
                     AMDFAKRLLDYGVHAPTTYFPLLVPECLLIEPPETESKEELDAFVAVMKIIREEASKQ
                     PDILKAAPHTLPVKRLDDVKAARELDLNYFATHE"
     gene            12483..13727
                     /locus_tag="I9760_000084"
     CDS             12483..13727
                     /locus_tag="I9760_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HAT5041887.1"
                     /translation="MVVELDETPVRTLLARQGIYDKNSAIVAYELLYRNSDAENSHVD
                     NLNQSSGEAATSSVLVQLFANLDVNTIIGNKQAFINFTHSHLVQKIPMLLPKNRIVIE
                     VLETVAIDQPLLLNLMELKKQGYQIALDDFVFRNELAPLVDMADIIKIDVLHLDQQQI
                     AEQLLPLKSFRGKLLAEKIEDKQQFNHCVNLGFDFFQGFFLNKPDSLTGQIITENKIQ
                     LLRLLTEINNEEVPIQRIEEIILQIPKLSYRILRLANSASLYMSKKIESLMDAISQLG
                     LMQIRNWLNLLLLASLDDVAPDLLERTLIRAKMCESLAKSMDYPNSHQAYTVGILSTL
                     DGILNEPMPSLLAKIRLSETLNEALLNYKGDLGKILKFAIDYEQANFNQLERIPVKSE
                     TLSQSYLKGIEYANHVIDIINK"
     gene            14093..17311
                     /locus_tag="I9760_000085"
     CDS             14093..17311
                     /locus_tag="I9760_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041888.1"
                     /translation="MIIRELFNWIHNDSAPLHLSDQTIERDLIEQEESQAKQTHRVLL
                     GNVRLLKYSGSSNTEAIKELEQHCLFMDYLQLYKQEFVKDGKNTVFLIALRNLLSHSH
                     EEQLRLMPKINELFQILLRDNKESALSFYDKNKELFRHCTEIQERVTFELFQQKIEAD
                     SKSVMTQLDYFNEHLAYQENPLGIIALCRNWIGETEKFTALILWLLERKVSVEKILLT
                     NLLQDFLRYHLFTLHSRDNEVSRLYSLLGHFPEAQELVMAAQKISCGELMFQQYSLDG
                     TFRGKNLPVVSPEIPPLQFSLSKDNFIALHRTFGPAFLTAGIATATNHDEVAWLDMLR
                     HTLNQPETLKLLPDIINIIAREYSPKVLKTLAELIAESTAHQLLTLNQSCVFHLLQHK
                     PRLLHDITEENVIGYINHLVRLDTHDQEIIYQLMALFRVLLKKNHPATKAVFEAILDN
                     LVNHPQLLEDEEFLTQLKKYPDCELLLDERCENILKQLNFCIAEQTSGLLFGKHNYFI
                     IEDVWLGALRKFAVLQQINPQMKFSFGHKYALQARIAEVVFIHQGDLFDLDTFIDALD
                     LPLVTSSGEISPYERALIEILATIDNELIRKQIIQKLETTPFNRLDWHEREYGNQTVF
                     IKAAKKGNLGLINLLEDKMEPSVLNKALRAAAKNYQWETLDHLCSLPGVELRQDEIDD
                     LVVYLAEHGRIESVKKLLKLYDYKPSTELIGTILKKAIDNDNLQVVMYFCKLPVESPK
                     QSMLDRLFKLAIQLQHWDIVEYLANSKHYSPSQTTLEKAFQQTALAMQHEAVEILGNV
                     EKNPVRAIVIERALLKASKLGHTKVVQSICALPSESLTKQAIEDALEQAAAEGHLDIV
                     SCLCEPGTTTLRQPGINSGMKIAVQTGKLSVVNYFCSMTGSNKPTPRLIDQTLVMAAK
                     KGQTAIFMAIHSNRQTPPGKHAIEQSFQLAITAGKLQILDYLCRHESYGLNQSRIDQA
                     LISAVKSKQLGIVSYLCESLEITPSRKALKIAVSKAISSDQTELADYLRSRSSGKSKP
                     LDTPTDEMASPLEIGKQLATNGLFKYKRKDAKEPPLLLNPSL"
     gene            complement(17617..18654)
                     /locus_tag="I9760_000086"
     CDS             complement(17617..18654)
                     /locus_tag="I9760_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HAT5041889.1"
                     /translation="MVMRSFSFYYQHLENVSRSFSFCISQLTYPAKEWVALSYLLLRV
                     ADSIEDAKWQDLQTQSDSFAAFKSFLVQTPANDQFTKWLSSFPSQLPLGEKQLLCAVP
                     LLLEEKDNLPESIKQSVIKTILQVVDGMHYFLNHYHMEGKIVFPSLVTTNQYCFFVAG
                     LVGKLLSHIFTDLLSNFKWTASLLNQAFHFGFFLQKINLLKDRVDDETSGRYFISSEN
                     SLRESLVIHAHHSLAYVKSIPIIEGRPYRLFCAWSLFIGLASLKWLDKYGHRQKIKPR
                     ETYYLVNQINLLIDDNRALEKLFNSYLPEQCKERSRYISESSKQIPAWFKKIYDGEMD
                     SLTLFELDVQV"
     gene            complement(18654..20456)
                     /locus_tag="I9760_000087"
     CDS             complement(18654..20456)
                     /locus_tag="I9760_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAT5041890.1"
                     /translation="MIQLGDLLKPTWAAERSLNNAWSVLNDEEKETIKSRMDKIFYNE
                     IPFQLEHDKLIYIHLFSLFAQLETIGLRGLIKSLEKLRGTDLYQQMRQQITDEIFHAT
                     VFAKVAFQLSAPYALPLGHQKSINHFISSLEGEEDLATSITLVNLVGEGWVEELCIAM
                     KEKNIAVTIFETVLEDESRHMDEYDLYRQIGLPNKDYLRKKLAIFEDELINTVFAHEQ
                     YLTTLGILLGKEGALKLLNNINNKHHWMLKKIGLTPSAHWQLFMDTMPLLMKNLSHDF
                     EKDKAIEPTNIRKLLSAIWNDPELPTESAIFNINVTPVCFFEKKFKPETITCLMLQAL
                     SKACFDNPQTRNYIFNHKLYHSHNSYVALAVKIPGSDQLGAIEFKNCHEMTMTELAQH
                     IQHDMRIMKYCYEKTQSLQKEHPYLIEVVNRLLTPRHERVYRDFLFARPAISLSNIGH
                     WGYQAAVSPLFPNETFKITLTEIERKQVWNKTNNTFEVQDVLPVGMSVDHRVFDGNIP
                     FPRYMQEAFDQMFQDMEQSRIKPLSKPFSNLDSFIKYSNTLLENDLEFGFLYLFSLMH
                     VWKNYISYDELSKTVEENYERIKQALSKSEHQLG"
     gene            21084..21353
                     /locus_tag="I9760_000088"
     CDS             21084..21353
                     /locus_tag="I9760_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041891.1"
                     /translation="MNSKVFSQRFNRELATLGFPEDLTEKTKAVSKVFGVTRHLANAM
                     IFGHVLPDKEQLDKIAEILEVCPQWLSGATDRKKAYSGRETADSV"
     gene            21452..22258
                     /locus_tag="I9760_000089"
     CDS             21452..22258
                     /locus_tag="I9760_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HAT5041892.1"
                     /translation="MECLSFCVAKTIDLTRLDLHLKNVSKEFSAVKTRDVIRLNSHRN
                     KDHTLFIFKNGTVVSWGVKRYQIHEYLDIIKLLVDKPVALLVHDEFHYQIGDKTAIEP
                     HGFYDVDCLTIEEDSDELKLSLSYGFSQSVKLQYFETIIDALIEKYNPLIQALSHKGE
                     MPISRKQIQQVIGEILGAKSELNLISNFLYHPKYFWQHPTLEEHFSMLERYLHIQRRV
                     NAINHRLDTLNEIFDMFNGYLESRHGHHLEIIIIVLIAVEIIIAVMNFHF"
     gene            22276..23556
                     /locus_tag="I9760_000090"
     CDS             22276..23556
                     /locus_tag="I9760_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAT5041893.1"
                     /translation="MPTLKSYFLKDTSLRTEILAGATTFLTMVYIVFVNPKVLHDAGM
                     DQGAVFTATCLITAFACALTGFIANTPIGVAPSVALNIYFSYSVVQGMGVDWQHALAM
                     VFISGLLFLFLTLTKLRRLLVEAIPHNLQIAIVIGISLLIALIALENNQIIIGDQHNL
                     MRLGSIIRPETGLFFLGFILILTLDYHKIPGAIIIGILSISVLSLILGLTSWQGLIAM
                     PPSLEPTFLQLDFSGLSSIVALKTIFTFFLITVFDATGSLIGLLSPTIFKHEKNYYKR
                     LTRSLTADAAASSLAGLMGSASTSPYIESATGIEAGGRTGLTALVIATGFILMLFLFP
                     LAKMIPSFAVGPALLYVACCMIKHITNMKLQDITETAPCMVIIMMVPFTASIADGIGL
                     GIILYTLLKLFTRQKPNPLIIVLSLVFTVFFLIN"
     gene            23859..24455
                     /locus_tag="I9760_000091"
     CDS             23859..24455
                     /locus_tag="I9760_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAT5041894.1"
                     /translation="MLEQSLRHYYQLVLVDPILPALRDRITPLSITWLSGIFGLLFIP
                     FVLLDKPYIAISFLLLSGYLDTLDGSLARFQNRASDFGSAMDIVIDRIVEFSAVLALY
                     LFDPQHRATLAILMLGSILLCITSFLVVGIFSNNTTQKSFYYSPGIMERAEAFLFFIF
                     MTLFSSCFNFLAALFCFLVCLTALIRLFEFKKSVQAPS"
     gene            24563..25138
                     /gene="msrA"
                     /locus_tag="I9760_000092"
     CDS             24563..25138
                     /gene="msrA"
                     /locus_tag="I9760_000092"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAT5041895.1"
                     /translation="MLRILLYFLLLINTTFCLAKTEKAIFAGGCFWCMEADFDHLPGV
                     LSTTSGFDGGTLKDPTYEQVSAGNTHYAESVMVEFEPNKLSYKKLVEYFFRHIDPTVK
                     NAQFCDHGSQYRSAIFYLNDQQKKIAMEYKKELEQKFPTVYTEIAPSTSFYPAEEYHQ
                     NYYKKNPIRYKYYRYRCGRDTRIQEVWGNKK"
     gene            25257..26117
                     /gene="vipF"
                     /locus_tag="I9760_000093"
     CDS             25257..26117
                     /gene="vipF"
                     /locus_tag="I9760_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector N-acetyltransferase VipF"
                     /protein_id="HAT5041896.1"
                     /translation="MITQVNQLDELQLKDLKTLRAECKKNDGSIPNLYIHILKQHRSL
                     PTSFLYYQNGALIGFLSIYFFYDDAVEVAVLVSPQYRRQGIAKQLIKEALPLIKSQNY
                     FNLIFSCPSRLNDNWLTSKGFTYLHSEYFMERDDLNPILDYIRPLSFRMATLEDIPIL
                     CGLDEVCFPDKNQDSVHRFQQILNEREYEIVIAMLNNHPIGKSHIRWQTKRATLSDIA
                     ILPKEQGKGFGSALIAHCINMILSEGKSRVDLDVETHNKKALNLYIQLGFHIQNACDY
                     WSINVNQLAK"
     gene            26242..27471
                     /locus_tag="I9760_000094"
     CDS             26242..27471
                     /locus_tag="I9760_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HAT5041897.1"
                     /translation="MKKRVVITGMEITSSIGSGIDKFWLAASQGQCGIKRIQSYDPSP
                     YSTQIAGEITDLSLDHLPEFNKSKRYPRAAQYALFCAHHAIEKAGLEQAELNAAGTFI
                     GTSIGGQPELEAGYRLFFTDSWKKIPPLSVIRGMPNSIANHIAIAFGIGGPNSTISNA
                     CVSSGEAIGMAYQQIAHGNLTTALCGGTESLVWESVMAAWCKLRVMSTNNENPSQASR
                     PFDLNRDGMVMADGAGILILEELQQAKARGAQILGEIIGYGASCDAFHVTAPNSQGQA
                     RAIVAALNDAKLSINDVHYINAHGTGTQLNDSTETETIKMVFGKRAYDIPITAQKAMT
                     GHAIGAAGAMEIIATTLSLQKNTLLPTINLHTPDPACDLDYVPNAAREKTIDIALSNH
                     FAFGGANAALVLRKYIS"
     gene            complement(27468..28250)
                     /locus_tag="I9760_000095"
     CDS             complement(27468..28250)
                     /locus_tag="I9760_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HAT5041898.1"
                     /translation="MLKKILLTGLCVLFSMNLSASEPIYDEEIPLLWSSIITVSGGPS
                     WATPGQNQYIYPMSPVYINNFNPNLPPNYYLYFTHDSPTTILANGEIFFGLQRAINPQ
                     MIGQLGLGVAGVSDAKVTGDVNLNGLLNVYTYDYKVNHARVELKGKLIGSAFTPVQPY
                     LSGSFGIAFNNAHDYRTLSINQFLFPAPWFASNTTYAFAYSVGAGVQKMLNRNWQVGV
                     GYEFADLGKSYLGGDGVNITKGPRLTHLYNNQLLFSVSYLYS"
     gene            28514..29569
                     /gene="lpxD"
                     /locus_tag="I9760_000096"
     CDS             28514..29569
                     /gene="lpxD"
                     /locus_tag="I9760_000096"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAT5041899.1"
                     /translation="MNSSLRDIANLVQGMVIGNDAITVSTLSPIDNILPGSLVFAEGE
                     DNIKLAENSEAAAILIGQGTIDSPKPLIQVKNPFKAFIALLNHFYPPRRISPGVHPTA
                     VIGAEVQLGDEVYVGPFVVIESGSIIGNHSVLKSHIHIGHNVVIGDHTTIHPQVTIYD
                     NCRIGSNVTIHASTVIGSDGFGYTFVDGQHLKVPHSGYVVIENNVEIGANTAIDKATL
                     GATVIGEGTKIDNLVQIAHSVKLGKHNIICAFTGIAGSTTTGNNVIFAANVGVSDHVH
                     IEDEVILGARTGVPPHKHLKKGTVYLGNPAKPKDVAIKHELSVNRIPLIRKNIKSLTE
                     QVAVINKKLDIKAKEVE"
     gene            29572..32262
                     /gene="polA"
                     /locus_tag="I9760_000097"
     CDS             29572..32262
                     /gene="polA"
                     /locus_tag="I9760_000097"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAT5041900.1"
                     /translation="MKPPLILIDGSSYFFRAFHALPPLTNSKGQPTGAIYGVANMIKK
                     IIKDYQPEEIAVVFDAKGKTFRDEWYPEYKAHRPPMPQELSSQFQPLIQLLQAMGLPL
                     LIIDGVEADDVIGTLAKQATEQGIPVVISTGDKDMAQLVNEHVSLINTMSNYSMDIDG
                     VKAKFGVTPEQIIDYLTLIGDSVDNIPGVEKCGPKTAVKWLTEYQTLDNLLAHANEIG
                     GKIGEYLRASIPHLPLSKKLVTIKTDLDLPLNLNQLMPKPADSEQLIKLTRELEFKSW
                     LKELLQEEENQKSPKEMDASNNKSYEIITTHKQLNHWLNKLEQSQQFCIDTETTNLDV
                     MQAELVGISLAVEEENASYIPLAHTDGSTQLVKEEVLTALKPILENPAIGKIGQNIKY
                     DYSVLKNYGITLKGIGYDTMLESYVLNSGAGRHDMDSLALKYLGYKTISYEDVAGKGA
                     KQLRFDQIPVVKAGIYAAEDADITLRLHHKLYPMLDESLRNVLHDIEIPLLTVLADME
                     MHGVLIDPVILEKHGSRLKEQMKSLEQEAVQLAGKAFNLNSPKQLQEILFDEQKLPVV
                     AKTPTGQPSTAESVLQELAFDYRLPAVILEYRSLSKLVSTYIDALPKRINPKTHRVHT
                     SYNQAVAATGRLSSSDPNLQNIPIRSEEGRLIRTAFIAPEGSLIMAADYSQIELRIMA
                     HLSQDDNLLRAFANGWDIHAATASEIFQTNLDAVSKEQRRRAKAVNFGLIYGMSAFGL
                     AKQIGVERQDAQHYIDTYFRRYPKVLEYMERTRKQAHQLGYVETLFGRRLYLPEINSR
                     NLMRQKAAERTAINGPMQGTAADIIKKTMLAISSWEHSQKNPSAKMIMQVHDELVFEV
                     RKEAVEECRAIIHKLMEQTVKLSVPLVVSIGTGSNWDDAH"
     gene            complement(32292..32744)
                     /locus_tag="I9760_000098"
     CDS             complement(32292..32744)
                     /locus_tag="I9760_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT5041901.1"
                     /translation="MRVLIVEDSAFNAFCLRRLLESVVASISVTIVNNSQAALSFVNN
                     YCPDIVIVDGDLGASPDEMNCNGPELAGILLQKYPQLPIVAWSDSDAMRENFIKVFKS
                     HGLFLNEYNTWPKVIGLERIHKTWSYYFGEFIGGQNPSFASQTSINYY"
     gene            complement(32859..33200)
                     /locus_tag="I9760_000099"
     CDS             complement(32859..33200)
                     /locus_tag="I9760_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAT5041902.1"
                     /translation="MNQLPNCPKCLSEYCYEDGDVLICPECTHEWPKNSNNNDEESAK
                     IIKDANGNLLQNGDTITVVKDLKIKGSSQVIKVGTKVKNIRLVDGDHDIDCRIDGIGA
                     MKLKSQFVKKI"
     gene            complement(33361..34554)
                     /locus_tag="I9760_000100"
     CDS             complement(33361..34554)
                     /locus_tag="I9760_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAT5041903.1"
                     /translation="MPKVIIFTDFDGTVTGKSGNETVFTEFYQSLLQGYKKDVEQDYK
                     NTPMKDPIEAQALFEAKYGKYNENFDHDQQDVDFLMSPEAVAFFHEVLKNDDVTVNIV
                     TKNRAEYIKAVFKYQGFSDEEISKLTILESGYKFNDVNSRLNHPTERANRVYILDDSP
                     TDYAEMLRAVKGKGYNEEEIRGYRKNPGEFEWSQYLEDVREMFPPKENSPEEVQLPDE
                     QGDEVSIGEETPVLLQEDNLSRREETATVLSNQPVPSSDFRTLKIMGAFAGIGFGFGF
                     ALGVTLVATGVFAPSGVGLLGALTLGAISGTGTAALSSAIGFVVAEGTEITSESVSEE
                     AVPAVKQSSHEAMRGLGGSQLLSTNNIHPVGHFPSILSRRTPTKGKEEQCQEEYVLTN
                     PLHNS"
     gene            complement(34744..36123)
                     /locus_tag="I9760_000101"
     CDS             complement(34744..36123)
                     /locus_tag="I9760_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5' nucleotidase"
                     /protein_id="HAT5041904.1"
                     /translation="MDTHKVFVNRIINMRKIKLIGLDMDHTLIRYNSKNFESLVYDLV
                     KERLAESFHYPEEIKKFKFNFDDAIRGLVIDSKNGNILKLSRYGAIRLSYHGTKQISF
                     SDQKKIYRSIYVDLGDPNYMAIDTSFSIAFCILYGQLVDLKDTNPDKMPSYQAIAQDV
                     QYCVDKVHSDGTLKNIIIKNLKKYVIREKEVVEGLKHFIRYGKKIFILTNSEYSYSKL
                     LLDYALSPFLDKGEHWQGLFEFVITLANKPRFFYDNLRFLSVNPENGTMTNVHGPIVP
                     GVYQGGNAKKFTEDLGVGGDEILYIGDHIYGDILRLKKDCNWRTALVVEELGEEIASQ
                     IRALPIEKKIGEAMAIKKELEQKYVDLCTRSIDESSQQYDQEIHDLQLQISTVDLQIS
                     RLLQEQNSFYNPKWERVFRAGAEESYFAYQVDRFACIYMEKLSDLLEHSPMTYFRANR
                     RLLAHDIDI"
     gene            complement(36187..36837)
                     /gene="rpiA"
                     /locus_tag="I9760_000102"
     CDS             complement(36187..36837)
                     /gene="rpiA"
                     /locus_tag="I9760_000102"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAT5041905.1"
                     /translation="MSELKIKAAKAAIAYIEDDMVIGVGTGSTVNFFIKELAAIKHKI
                     EACVASSKATEALLRAEGIPVIDLNSVQDLPIYVDGADEVNERGEMIKGGGGALTREK
                     IVANVATQFICIVDESKVVKRLGEFPVAVEVIPMARSFVARQIVKLGGDPEYREGFVT
                     DNGNIILDVFNLSFSTPMALEDSLNVIPGVVENGVFAKRLADKVLVASASGVNNLK"
     gene            complement(36830..37579)
                     /gene="rlmB"
                     /locus_tag="I9760_000103"
     CDS             complement(36830..37579)
                     /gene="rlmB"
                     /locus_tag="I9760_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAT5041906.1"
                     /translation="MNEQYVYGLHAVSALLANPHRMIKKLFVNQDRIDKRLHEFLVKA
                     ENAHVPIEKLSAQKMNQQFSEFTHQGVVALASALAEYGESDLVTLLEKSKKPVLILIL
                     DGITDPHNLGACLRSADATGVDFVVIPKDKSASITPVVSKVACGAAESIPLVRVTNLA
                     RAMDILKQQGVWIFGAAGEAENLLYAMDCSGNIALVMGAEGTGLRRLTREHCDGLFSL
                     PMMGSVESLNVSVATGVSLYEVIRQRGQFRE"
     gene            complement(37572..39752)
                     /gene="rnr"
                     /locus_tag="I9760_000104"
     CDS             complement(37572..39752)
                     /gene="rnr"
                     /locus_tag="I9760_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAT5041907.1"
                     /translation="MSKKSKDPFYKREREKYAEPIPSREYIMEILDEYGRPMSRSKLF
                     DKLDLSSESQQESLGFRLKAMLRDGQIMQDRRGRFCLLQKINLLRGTVQGHPDGFGFF
                     IPDDGSEDMVLSAKEMRAVMHGDLVLAYQSGLDRRGRPEAKIHEVLEHANTTVVGRFF
                     TDHGVNFVIPDSKRLTQDISIPQEFIGEAKNGQIVLVELIAYPNKRSQAIGRVIHILG
                     EHMAPGMEIQVAIHAHGIPFEWPDDVLAEVGKIPQQISEEQLKGRTDLRNLPFVTIDG
                     EDAKDFDDAVYCYKKPKGGFQLYVAIADVSHYVAKDSALDQEAARRGNSVYFPGKVVP
                     MLPEALSNGVCSLNPHVDRLCMVAELSITQEGKISRSRFYRAVIHSHARLTYTSVGAW
                     LEQGKADEKHQALWPMLESLYDLYHILLDTRRLRGAMDFETTETRIEFDENKKIKCIV
                     PVIRNDAHKLIEECMLAANVATARFLEKAEIPTLYRVHAAPEEDKITALRQFLGELGL
                     SLGGGRKPGPKDFQRTMNMIGDRPEKHLIETVMLRSLKQAQYVESNEGHFGLAYSAYT
                     HFTSPIRRFPDLLIHRAIGHLLDNHPVYEFEYSHEDMNRLGKHSSMTERRADEATREV
                     ITWLKCEYMQDKLGQVFKGRISAVTSFGIFVELDEIYVEGLVHVTSLKNDYYTFDSAK
                     HRLIGARGGQVYRLGDKMTVLVARVDLNERKIDFEPVEDEVCNE"
     gene            complement(40158..40622)
                     /locus_tag="I9760_000105"
     CDS             complement(40158..40622)
                     /locus_tag="I9760_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041908.1"
                     /translation="MRILLSFYLLLLSTGLFAESTFPDGCRAVPVQGQSVTLKAKKNK
                     LVFIHNVTNLDLWITHPVTDPGASAGWTSRLQAGKWSALLVDKPAFILNCIESRPGHE
                     QQIPCENAIAVCQWKGVKIPASDQGTFWVAEDLSLAALTAAVGGRGFVLPEK"
     gene            complement(40837..44811)
                     /gene="lem1"
                     /locus_tag="I9760_000106"
     CDS             complement(40837..44811)
                     /gene="lem1"
                     /locus_tag="I9760_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem1"
                     /protein_id="HAT5041909.1"
                     /translation="MKQLISIIKGQIQQLPIPDLKHLLLSHLKEDAHDLTELFCQQIP
                     AQKNQFSLNHSVPFGLPASLSVLTIFLTYAPNLLHRELSEQLLTMLDSIKETETSVDN
                     QLAERCTRQLNYYSDAKPVLIALKERGAKDLIQILEQADERSLSAFSHLAKDTDLNVL
                     IETFLVKNNKDLDLVNRNHLVQFLRQPDLAGWLTRFLDSLTDESINALSIRLLLSLVS
                     SEKAIEKLKEARVCLAQYPFSIDLHAMRAVFDFYQYELKRDLFSPESHLVMQLLHDLL
                     KNTKLDKQQEFFQGLSQETVLLIIEYCIAEYNKTTGEEKQNCKNLLTRLCSAFSDSSQ
                     DFLGEIRTKINQLDLYLFDTPQLYQLASDLITKSYNSGKEPLSSYPIALLLSMPQFVG
                     VCTPKVLKQLVERYSLYTLTLNQKDYKQDLCFKEYAPYFDEINEHMAQIERNLCIHPE
                     AQSFWLDKKYSLLQFRVEISKSALLHQMECLLEANKGGDELHNKGLEVLYHYYALSYP
                     NLRSDLFLKVVNHIYSRFFIKGNNETEARNGLFHWLKQSFPLIDKLPKELDRKKEIFL
                     YNSAGKKIGFLGESNAAMTFVNEEIVLLARTGSMDINEPLYDQEGLVLGYLTEESCLR
                     SESAIHKNAAAWVLAGLSKSDLESSSFGLESLLHNVLFENAIGVLYQCESICLDANKR
                     IWLNNKLLKEIQEAPGEIPASVLYPLLDHFEDETVFELLGVIRNKNNALELFHYILNR
                     DSRRSILLNGLYESDFQRFLEHQGADVCLADYLVNYYDKPWFSEVLLRFAYHGKKYKK
                     PDLLSSALTFITKEISQDKKQETICDAVLDRLINSEACAALVLKEFLGDRTHNSIQKV
                     NNPEIGKVSQFFDRRHIVPLIAQLNKTSYWEQNAQYKLALHILANQHGRLFSETDLNL
                     DSPQAWQSKELKELACFTSRHLSKKRPFDNKHDIGYRVLGELLFRCANAGITSLFYKH
                     KALNRAITRLSITEPFLVRMVDKLWMPKGIRELYGKSLFFKGSWFDDQVFLQKELKDH
                     HPLLDWSHFIKQTWSKRDKQKKSPIICSYLLSYTGKKSVLLHLLQDYFNSFQGMPDYI
                     RPVSQLLRFFPQRDVSAVIYDALEAEMIKKPQSLDRQILSDMAFYYGKQLARKDTSFP
                     QADINLLTYWGQNKHYSLVKRGCELLVRDCEDKGVKKRLISAAKEAEVEQDLSSSVQT
                     FYFGLIKFFKRLWHYGFNAENSSSKIIKFCDDRTPGPTRKKAGDLINAPVAESRTNSL
                     HLGFSERRKQLISLLATIKSSPAHFTRVVNYSDNTQTFFGEQSKQSIDYNTPAIPSNF
                     ESIM"
     gene            44922..45368
                     /locus_tag="I9760_000107"
     CDS             44922..45368
                     /locus_tag="I9760_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAT5041910.1"
                     /translation="MTIWIDADACPKMIKDILFRAAIRTNTNLILVANSYLTYPNSPF
                     IRSVLVEKGYDRADHYITSHMKAKDLVITADIPLAAEVIVKQGLAMSPRGELFTANNI
                     KQRLTLRDINEQLRSAGERTGGPSALSAKEKTNFANALDRWLAKSK"
     gene            45459..46190
                     /locus_tag="I9760_000108"
     CDS             45459..46190
                     /locus_tag="I9760_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAT5041911.1"
                     /translation="MKRFMFILLMFLNFYTFGKPLIIGVPEFAPPFVSRSGGGGETYF
                     GFNIDLMNSICKIIEMECQYKGMNIQQLYTNLNQGKLDIMLAPTPIVSTVGTNYIFSL
                     PYLPSNAQVITLKANNDINSLADLKGKKVGTLKYTLYGSLLAEHFQDYFDLVQFSKLS
                     DMALALSNQKIEAIVLNTNAAKYAMSNSATELKFVGDKMAIGDGYGILALKNNAPLIM
                     KINKALLQIEKDGTYLEIYNTYFGD"
     gene            46417..47007
                     /locus_tag="I9760_000109"
     CDS             46417..47007
                     /locus_tag="I9760_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HAT5041912.1"
                     /translation="MQESFDPSTITTLHKTSIIRWKANGIEYQHKQFLQLVEENHAFN
                     FQLWHAEDKARRDDMGFEFVYQAKREIDAYNQQRNNRMEAMDEWLYKALEPENHNHCP
                     VNSESPGMIIDRLSILSLKSYHMDLQTKRQDVSEEHRIACTQKLEIIQQQLNQLALCL
                     DELLNEVRAKTRTFRVYHQFKMYNDPKLNPELYCSD"
     gene            complement(46973..48898)
                     /locus_tag="I9760_000110"
     CDS             complement(46973..48898)
                     /locus_tag="I9760_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041913.1"
                     /translation="MPHASDENQASKPTDLVFILAIAGRIDAQSRILAQKLDKSRSIY
                     YAYAVMDSLSSSYSMFKYFFDLFASPNDNDAMHEFLNSPAGLITIAVESVFLVTFSVL
                     ACQFENEKEDYYKKFIANAWPYFRDVMKGLKNAYKGWRSAVVAFSLIGGVDAKFLILP
                     MGVILGVFAAANRFWIRSMLEDRKIMMSENIALLMEIKKLISLSHEESFSYFKRIRYQ
                     TMQTRNFAFWAVAAGGLLDGLYLYVGVLSLAVLSPQLLMAMALICAFYTVACVITRIY
                     EEYDFQLRLLVTQTKCQLALITKELETNYAKLLLLQDKMDLDKVDQEELKRLKRLVHN
                     LITRFDDSRKLLSQQSNRTYLSSALLGMKNGLYAYGALASVLFLVGSILVMAGVSFPP
                     ALLTACIILGLVLVLSFTIHSLVSNYWHQKKQETQKERPYERLIEWKNSLNDEFNKTE
                     LLAPEEFHKLLNDGLSLDPSPQFFFQEWFEVLRSLFSGFGKGQKFIDFAGNSLQELGE
                     DGHYRDTPVMYVLSVFSALLFGVTLALRALAKGLGRTPLAQQAKLTEDSKEASVSPEI
                     TKEVMQKTEEQRESIQSEKTRTDSPGHTDSLSLFGIFETKTKRNQPDSPSIPHSKSES
                     ALNQLIPSQNSTILGLA"
     gene            49148..49675
                     /locus_tag="I9760_000111"
     CDS             49148..49675
                     /locus_tag="I9760_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041914.1"
                     /translation="MRSKFVLFMLMSLLTGSLMANSEAQIYGYVEKATLIDQNLTLSA
                     KLDTGAKSASLHAVNITEIEKKGIPYLRFTVPTKTGDYSFEGEYVGKVKIKVRSSETN
                     PGLLRTTPIKRPVVLLNIKLGDKVRTIKVNLTNRKRFLYPLLLGRDAIIDFNGAVDPA
                     LTFTTKSKSPVNNEK"
     gene            49665..51188
                     /locus_tag="I9760_000112"
     CDS             49665..51188
                     /locus_tag="I9760_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HAT5041915.1"
                     /translation="MKNNKRHVYGLILTLLLLGSGIFLYRHIVLDVPLTDTETINSWM
                     VESNLRFTADRNTPIKASFNIPYLPPNFAILDEYFVSRNYGVTTNLNGSNRETVWSIR
                     RGNGPQSLYYRAIFRQTDSDESSLPKPSVTKSQPLNDSQKSAVETITNQVRSTSADIQ
                     TFAQSTIKELNKRDGNAKLLVGNEFNDDNIINATILILNQSKIPAITVQGIYLNQQKK
                     ADLKSLLAVFNGKNWIYINPKTGSAGLPKEFLIWQYGNGPLFNVVGGNRAQFSLTVSP
                     TPINALSVAKSRGLEDSQLLRFSLLQLPVNVQGIYKILLTVPIGAFIILILRNFIGIK
                     TFGTFMPVLIALAFRETHVAWGITLFVIIISFGLLARFYLDQLRLLLVPRLAAILTVV
                     ILLMIFISVLCQNLSLDTGMSVALFPMVILTMTIERMCITWDERGASEAIKSGIGSLA
                     AAVISYGAMSYEPLQYLIFAFPELLLVLLSLILWFGQYRGYRLVELKRFKSLASAMK"
     gene            51185..52132
                     /locus_tag="I9760_000113"
     CDS             51185..52132
                     /locus_tag="I9760_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HAT5041916.1"
                     /translation="MINLFRRLKNHGVLSINQRNTDFVLRYNPRKLFPLVDDKLKTKK
                     LALKAGIAVPALYDIIETEQQIKSIETRFASYNDFVVKPARGSGGDGILVFKDKVYGR
                     YRQINGKLTTVQELSYHLSCLLSGAYSLGGSSDYAIIEKRVVVDPVFAEVSYEGIPDI
                     RIITLLGYPAMAMVRLPTRLSGGKANLHQGAIGVGVDLATGKTLGGVFQNDTIDYHPD
                     TLNPIVGIEVPYWNQILEIASSCYELTGLGYLGVDIVLDKEHGPLMLELNARPGLNIQ
                     IANREGGLKRYRTIEARFKESGQESTLDKVSFCRKEFAR"
     gene            complement(52129..52587)
                     /locus_tag="I9760_000114"
     CDS             complement(52129..52587)
                     /locus_tag="I9760_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041917.1"
                     /translation="MFGRLWLRLPVNKPTLDLSKMVLGRMPAETTFESVKREFEQELR
                     RVGLKRFQENIHAEQLILEQLLKQREEMTKKIDEKCLRPALQRSENGYDVTGLYWSRM
                     QGELSKIQSKKERVELDETIDWLYARRRFLKEQNNLSLNKEPAHSASPAL"
     gene            52867..54192
                     /locus_tag="I9760_000115"
     CDS             52867..54192
                     /locus_tag="I9760_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0081 family Dot/Icm T4SS effector"
                     /protein_id="HAT5041918.1"
                     /translation="MRSKFFSFFTRASRPMKAIPPKIWFETQLKGSGLDKKFQIDELI
                     ETQSSVRVFANKKYLPDTETINEALTKVTAVNVSGDKSGYFQNGLPFPNEAGYFEKIP
                     VGHPELLSPIERLTGSKKIVSSHSLVTASGGYPLTNPLLPYRKPIRVSIFSLAGPSFE
                     NNYLHYRLFLLDSVQKIIDSPLFSHLHDGLPIQFDEAKKELGEYDTNKLMARIRLGFP
                     YLARFSSGGFYPSFSKSNAIIFLSEAYFRYQLEDVSLLLASVNQTGKETGKAALLKAT
                     AVGMGFFAKIDCGYDIQHIIFPYYLRAYKKLLSEHKFPWIAKIEFPIFNEIQQEQFDS
                     IFEDYDGPTKVYRSTRDVLEFREEEIEKYLPAAINPSDAFALTGNEWGYGSVESMIGN
                     NSSIRFDQVHHMNPLILDPSHHVEAQINKDHGVELTVNPRPQSSSSIKL"
     gene            complement(54182..54949)
                     /gene="ceg3"
                     /locus_tag="I9760_000116"
     CDS             complement(54182..54949)
                     /gene="ceg3"
                     /locus_tag="I9760_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg3"
                     /protein_id="HAT5041919.1"
                     /translation="MNRLKFFSKFHNALLKFESSISNLSDPLVGFKIKELQTKKILVR
                     ADGRSLDHLHKLGGLPQRVDSEKLEKVRITDVERYQKFNLNPFGWGACASTEDLRQFL
                     ETYPELTKQAWVHKFYGSSTSLLTLKSEVGIGCGEHDGEKEELVVDSVSFGQIIASTC
                     PKYRDKYLDGGVVPNAMKDFNPKVPETMKLGDFSSEKSTLEWLLINDCEEEFAKLCKE
                     VYGDTPLKILLRRFDGDQYLADAVTYYVKESSLSPRV"
BASE COUNT        17398 a        11446 c        10000 g        16186 t
ORIGIN      
        1 aatgcaagaa gaaataattt tgttgtcagt tagaagatgc atttcctcca aagatatcag
       61 ccttttctgc agaccagaat tagaaaaaat atacctcccc tcctcgtttg aaagtgtcaa
      121 taattttttt gtcaaactgg ctctatggat actggtatta aaaaggcaaa atcggcagtt
      181 tcgtttgttg agatcgtcta atttgtaagt ttctgtttta gaattatcgt gcggattgat
      241 taaaacaaaa tcatagttcc cagaaccttt aggaataatt ttgtctattc tcaaagcatg
      301 acggccatga aattttccaa agctatcgac ttttccataa gacatactaa tataaacagg
      361 ctcatcagga ttcattaatt tcaatttgat gatatcgtca agctttgaat atggaatgtc
      421 ttccgcttga ataccaaaga atttgcctaa aaaagcagtg gaagtaaaac cggcaattct
      481 gtccggtata tcatgagcaa caacgcttgc caaaggatca gtattactcc aatctcccgc
      541 ataataatag gaaaccaatc gttccaaaat ttttattgcc agcgaattac tttttacccc
      601 gccgtattga ttatcaattt cctttagtct ttccgggcta atctcaaaca catcttcatt
      661 gttgagttca tccacgtaat gggtgtactt ccctgtcatt ttattctttt gcaaattatc
      721 ttttagtgcc tcatggcgtt tgattcttac gataactttc ccatcttccg tttgggtaaa
      781 caacgattta attaattgct ccccatccgc cccaagattt aaaatgcaat ccagagatga
      841 caaaagatag caactacctc caataccttg tcggatggta atagggcctt gagttgggaa
      901 caaaggtgtc attgtggtcc tcttaaaatc ataacgtaca gaaagagggc attataacag
      961 caaaatattg gataatcatt aaaaaaaatg gtctgtaatc aatcggaaaa ccaaaaccct
     1021 aaaagctaat tccactcata ccaacggtca agcaaggaaa tcaactcctc aacaccatcc
     1081 tttttcacag aagaaaacgc ttgcacagaa accaaatgct ctgccagctc ataatactgc
     1141 cgaactttaa gtacggcatt tttgacttca ctacgactta acttatctgc ttttgtcagt
     1201 aaaatatgca ctgggagctc tctgtgcaaa gcccaattca ctaatatttg atccaaatct
     1261 ttcaatggat ggcgtacatc catcaacaaa attaatcctc tcaaacattg ccttgcttcc
     1321 agatagtggg ctaaattctt ttgccattcc aattttactt ccattgccac cttggcataa
     1381 ccataacctg gtaaatcaac aaggcgccgt tgctcatcaa gagaaaagag attgatcagt
     1441 tgagtgcggc ctggtgtttt gctggttctt gccaaatttt tattattagt cagacaattt
     1501 aaggcactgg attttccagc gttggaacgg ccagcaaacg ctacctcata cccggaatct
     1561 tcaggtaatt gattgacacg ggctgcactt tttaaaaata ctgctttaga atataaattg
     1621 attggcataa gatatcattt tagttttggg attttgaccc aggcagtgta ccattaattg
     1681 actaaattat tatgagaaat ccatgaaaaa attagcactc gtattaatcc tgtgctcttc
     1741 atcagtgatt tttgcccagg aaaacccgca agaggctgga caaaataagt caacagtctg
     1801 taccgcctgt catggtccgc aaggtattag tacgaatccc gaatggccca atcttgccgg
     1861 ccagcatgaa aaatattttg tgaaacaatt gaaagatata aaagaaggta agtcgcgctc
     1921 tgcgcccact atgactgcaa tagttgctaa tatgaatgaa caggatatgg atgacttggc
     1981 agcctattat gctaaaatgc ccgttgctga aggttcaacc cctgaaaaat atttaaaaag
     2041 aggcgaacaa ctttacagag gtggtgattt aaacaaacac atcgcggcct gtatagcctg
     2101 ccatggtcct aaaggaagtg gaaacgctca agcaggcttt cccctgcttt ctggtcagca
     2161 tgcagcttat actataatgc aactgcaagc atttaaagac gggaagcgaa cgaatgactt
     2221 aaatcaaatt atgcaagata tcagtagtaa aatgagccca gaagatatgg aagccgttgc
     2281 ttattatatt caagggttat attgaggaat tgactatgtt taaaaaatta attgccctat
     2341 tatttcttat gccaatgact gctttggcaa ctcaatttat tgaaggcaaa gattatcaaa
     2401 ccgttgccag cgcgcaattg tctaccaata aagataaaac acccctgatt accgaatttt
     2461 ttagctatgg ctgtccatgg tgttataaaa ttgacgcccc actaaatgat tgggcaacca
     2521 gaatgggcaa aggcgcccat cttgagcgcg ttccagttgt ttttaaacca aactgggatt
     2581 tgtatgccaa agcctattac acagcaaaaa ccctggctat gtccgataaa atgaatccca
     2641 tattattcaa agcgattcag gaagataaaa atccactggc aacgaaacaa tcgatggttg
     2701 atttttttgt tgctcatggc gtagacagag aaattgccaa gagcgctttt gaaaattcgc
     2761 caacaatcga tatgcgcgtt aacagcggca tgtctttgat ggcgcattat caaattaacg
     2821 ccgtaccagc ctttgtcgtc aataacaagt acaaaactga cttacaaatg gcagggagcg
     2881 aagagcgttt atttgaaatt ttgaattacc tggtaagaaa atcagcctaa ttaactcaag
     2941 ctataaagga tattcctcgc cacggtaatt ggccacaggc caggtcattt tgacctggct
     3001 ttcttgctaa gcccccttgc ccgggttttc cagactcaaa ataccggtat tgaaatcata
     3061 agcaaaaatc tcggcatatc gcccccagtc aatcattgtt ttcaaaacac gatctgcttc
     3121 tttctcgctt aaataatcct ccagcttact caaaaagcgc tcttctgaga cgcgatgccc
     3181 cgctttctca tccagtaccc gtcttatata acgagccaga gggacttttt ccaataatct
     3241 ttgcgcgaac aattgcttac gctcttgcaa atcagcttct gaaaattgtt ttcctaattc
     3301 acttaactga atatctcccg cagacacctt ggcaaaacca agtatctcca gagtttcaag
     3361 aattgggaac agatcatcaa tattcatcat gagctcgtct gccagctcag gtaaatcaat
     3421 acgttcttca aaggacttca tagtttctat cagaccggat aattcagagg gctcgacatc
     3481 gggtaagcga tagcctaaac ctatttgacg ctccctttgc gctcgcttgg ctttttcttt
     3541 gggcccagta gtcattaaag tataaatctt gtcaaccaag gcaaggtatt ccgggctttc
     3601 cggatcacgg ggttggggaa gagtcactgg taattcagca cgaatatagc cgggatcatt
     3661 accaaaaatc actatcctgt cagccaaagt cgcagcttct tctatattgt gtgttactaa
     3721 taaaatacca ttggtattcg ttttcttttc tttccataac tccaataaat ctgatttgag
     3781 gttttctgca gtaagtacat caagcgcgga aaagggttca tccattaaca acacatccgg
     3841 attaataacc aaagcgcgag caaaaccaac ccgttgtcgc atcccaccag ataactcctt
     3901 gggaaaagcc gattcgaaac catccaaacc tataatatct atcgcttcaa tagccctgtg
     3961 ccttctttcc tcgcggctga ttccttgtgc ttccaaacca agctccacat tttccaggac
     4021 tgtaagccaa ggcattaaag caaacgactg aaataccata gcaatacctt ctaccggcct
     4081 ggttacaggt ttaccgcggt aagtcactgt tccactggaa ggagcgataa gaccagcaat
     4141 aatcctgagt aaagtggatt tccctgaacc agatttgcct aacaaagcaa ctatttcgcc
     4201 ttcctgtaat ttgaaattaa catcttcaag tacaagaagg tgctgtgaag gtgctttttt
     4261 aaaagatttg cttaaatttt ctatgttaat aattgtttcc ggcataggag tctcaattaa
     4321 aattaaatcg ttcttcggct aatcgataaa gcgggcgcca aataagatgg ttgaaagcta
     4381 atacatagac acacatcatt gctgttccta acgcaatttg cgggaagtct cccgctgtcg
     4441 tactagcctg aatatactcc cccagacctg tggccttaag agtaatattt ccccagctca
     4501 cgtattcagc gacgatactg gcattccatg caccgcctgc tgctgtaata gctccggtaa
     4561 tgtaaaaagg aaaaatacct ggcaaagcca atcttttcca ccatatccaa ccctttaacc
     4621 caaaattatc agccgccaga taaagatcgc gcggtatact cgaggctcct gcgatcacat
     4681 taaataaaat ataccactgt gtgcccagga tcatgagcgg agttacccaa atttccacgc
     4741 tcaaattaaa tttgacgata gcaattacaa ataaaggata aaacaggttc gctggaaacg
     4801 ctgccacaaa ttgaataatc ggttgtattt tttgagccag ccttggcctt aatcctatcc
     4861 acacgccaac aggaatccat agcatagagc ttagcaaaat taatataatg acccgagatc
     4921 ctgttgctgc gcccaataaa aagacatgaa atatatcact caccttcaat tcagcgagaa
     4981 taaatttcaa caaaaaccac ccgccagtaa taacggctat caaaaccaac gctgtccaca
     5041 atcggtctaa acgcttttgc cttttaaaat caatttcctt aacagccttc acctcaccaa
     5101 tcctcagcca acgagcattt acaaaactat tggtaaagac cgcgagccac tccgtcactc
     5161 tcttcatcaa acgactactg cgtatcaagt caattaacca ggattgatat tctgactcat
     5221 cgggagactg ttccatttta aatttttcag accaggcaat aagtggcctg aaaagtattt
     5281 gatcatacaa aaaaataacg ataaccatgg tcagaatggc atatcccaca gcatgcaaat
     5341 ccctctgggc tatcgccaat gcgatataag aacctacacc gggtaatctg atattttggt
     5401 gcgccaccga aatagcttct gacaaaacca caaaaaacca gcttgccgac attgaaacca
     5461 tcatattcca taacagtcca gacatggaaa aaggaacttc taccttccaa aagcgctgcc
     5521 aggctgacaa cctgaacatg gcagagactt caatcaaatc atgaggaact gtttttaaag
     5581 attgataaaa tccaaaagtc atattccaca cttgggcgct aaaaatagca aaaattgagg
     5641 cgcattctgg acctaacaag ctattgggaa ataaatgtat aaacccggtg acagtaatag
     5701 ataaaaaact cagaacagga atcgattgta aaatatcaat cgccggtata acaatttgct
     5761 cagccctgcg atttttggca gctaaagcac ctacaataaa agtaaacaga attgaaaaaa
     5821 tcaatgcgat aaacatgcgt aaaactgttc gtaacgcata aaagggaaga ttggaaggtt
     5881 ccaaggaaat gggaatttga tcccctaatt gatacggggt agccatctgt gaaccggccc
     5941 aacccaggaa aaataacaca gaaaaagtca atataagcaa tagcaaatcc catcgattaa
     6001 taaatcgact gaatttatca ggattagcaa aataaaaccg actattgccc actataccct
     6061 ctcttaaaat tgacgttaca agtttccttt aatctaatcc ttaaatctaa aaaatattta
     6121 tccattcctg aatcaggatt tacgaaaaac cccaaggtca aggcaaaaaa gtttttaatg
     6181 agggagttta gataaactaa acgaccgaat taaaaacttt tttaacaaag aaattggggt
     6241 ttttcggaag tcctgtgaat ataaaatacc ataaatacaa cgtcatcgat accagacaaa
     6301 ccaacacatt caaccgtatt cacaaataat tttgattaaa ttttgaactt tttcgcgatt
     6361 catgtgtcta atatagtgag cattctcccc cgagtgctct accccgcttt ccccctcttt
     6421 atgcggggta gtattcggat tgcaccaaat ccggatatat aattctcccc gggatcttag
     6481 ccggggattt ttttgtaatg aaaaaatgac taaactggca gtattgagat aatcgatgag
     6541 aatttattat gaacaaaaca atcacaattg ccttgagttt acttctaagt attgcctcaa
     6601 aactaactta tgctgggcca gatagagcac aattggcagt ttgggctaat gaggcgatta
     6661 ttgccactta cacttttgat tataaaaatt acatgcaaca acaaaaggaa atagccaaat
     6721 atttttcagc tgatggctgg atagcctata gcaaagcgct taatcaatca aaactgcctg
     6781 aagtagtaca aaaaaatgcc tatttcgtca atgctgttgc cacagaacca cccaagctga
     6841 tcaccctgga tccgacacac tggcaagcta tcatgcccat actggtcgta tataaaaacc
     6901 cccaatacga gcaaaaacaa aatttaaaag ttgtattagg gtttaccgta gcatctcccg
     6961 gacaaggtgt aagaggtttc agcgtaacca gcctgcaatc cacccccatc agcccgccat
     7021 gccaatgtaa aattgaagag acgcctggga atactaaaca aggagacgcc aaacaataat
     7081 accaatatgc agcaaaatta atgagctgat tagaattaaa ataaaagtta gttttttagg
     7141 tatggtaaca tctaaagcag attgttcagg acgattggga ttatagtaga cgtcaatttc
     7201 tgagttatct ctgtaagcta ttgccacttt atatgcaata cttcgggaat atttactgtt
     7261 gggtgtatta aatgttgtat ccaaaaacaa atattcccct gtcaaatcct tctcataaac
     7321 ttcatagata tattctattc taggccaaac actatgccct actcttgtcc actcacagcg
     7381 cgtgacatgc cccttcgctt ttaaccagga tcttgcttca accaaatctc ttcttttttt
     7441 ccaaaaatac caaaaaagaa ttaaaagaaa acatagccat cctaaatcaa ggatccatct
     7501 ccaaattatc ggccacacca tattctttcc cataaattta tattatatgc tatttccagg
     7561 gagaggatca tgcaaaaaaa tcaaattgga aacctcgcaa aattaatccc aacaggtata
     7621 atcacatcca ataagtgata aaaaaaggaa atgcaatgac tgctaaaact cctcttcaca
     7681 ctacgcactt agcatgtggt gccaaaatgg tggatttcca tggttgggat atgcctcttc
     7741 attatggatc tcagttaaac gaacatcatg ctgtcagaaa cgatgctggc atgtttgatg
     7801 tctcacatat gaccatcgtt gatattttag gcgccggtgg ccgtcaattt ctgcgcaaac
     7861 tgctaaccaa cgatgtcgat caaattactc ataatggaaa agccctatac agctgtatgt
     7921 gtaatgaaca cggcggaatt atagatgacc tgattgtata tcaacgcgct tccgataatt
     7981 accgagtggt acttaattcg gcaaccaggc aaaatgatgt ggcctggatt cgcgcaaaaa
     8041 gtgaaggatt tgctgttggc ttacaagaaa ggcgcgaatt atcaatgctc gctgtacaag
     8101 gtccaaatgc catagcgaaa acattgagta ttcttgcacc cgcccatgta gatgccgttt
     8161 cgactcttac ctctttcgaa tgcgttgatg tggatcactg gttttttgct cgcaccggct
     8221 atacggggga agatggcctt gaaatcattg tccctaatga atttgttacc caactctgga
     8281 atgatctcct gcatgctgga gtaacgccat gtggattagg ggccagagat accctgagat
     8341 tagaggcagg aatgcttctt tacggccagg atatggatga aacaacaacc ccactggaat
     8401 ctggtttggc ctggacagta aagtgggagc cggaagatag aggatttatc ggaatgggcg
     8461 ctttggtttc tcaaaagcaa caaggaatta aacgtaaaat ggttggttta accttgttgg
     8521 acaaaggcat tatgcgtcat ggccaaaagg ttattattga aggctgccct gatggaatta
     8581 tcacaagcgg cagctattct cctaccctgc agcaatctat agctttggca cgagttccca
     8641 tggaaacagg ggaacaagta cttgttgata tacgcggaaa gttaatccct gcaaaagtag
     8701 ggaagcctcg ctttatcaaa caaggaaaac ctgtttaaat tttagtaata ttaatattaa
     8761 ggatataaaa aatgaatgat ttgaaattca caacaaccca tgaatggctg agagaagatg
     8821 aggaagaggt cacagtagga attacagatc atgcgcaaga attattgggg gatatggtat
     8881 ttgtagaact acccgaaata ggtgatgaag tgagtgcagg ccaagaatta ggcgtggtgg
     8941 agtcagtgaa agcagcttct gatttttatg ctcccattag tggtgttgta acggcggtta
     9001 acgaagccgt tggtaaaaac cctgccctgg taaaccacga tccctaccat gaaggctggc
     9061 tcgtgaagct aaagcccagc catcctgatg aaatcaaaag cctgttaagt gatgagcaat
     9121 atcaaaatga gatagctgag gaaaattaat catgccttat attccacaca cgcccgatga
     9181 taccaaagag atgctagagg caattggtgc tcaagacatt caggatttat ttgatgaaat
     9241 accagcttct ttgcaatatg caggatttca aaatatccct gctggcatta acgaaatgga
     9301 aatgttgaaa gaagcccaga atcaagccca aaagaatcgc aacggtatat gttttattgg
     9361 tgctggatgt tatgagcatc atattccagc agccgtgtgg gatattgcat ctcgcggtga
     9421 atttttaact gcctacacac cctatcaagc tgaggccagc cagggaactt tgcaattatt
     9481 gtatgaatat caaaccatga tctgtgagtt gaccggcatg gaagtatcca atgcgtcaat
     9541 gtatgacgga gcaactgcgt tagctgaagc agtactcatg gcagtccgcc ttaataaaca
     9601 cagtaaaacc aacagagtac tgattgcagg cacagttcat cctttttatc gcgaaaccat
     9661 tgaaacgata gtacgtaatc agcatattga agtcatcacc ctcccttttg atgaacagca
     9721 aggaatcact aatcttggct ctctcaatca atacaccgga gaagatatta cagctttggt
     9781 catagcccaa ccgaatttct ttggttgcct cgaacaagtc gataaaatga cttcctgggc
     9841 acatcataac aaaacaatca gtgtagcttg tgttaacccg acttcattgg ctttattaaa
     9901 accacccggc tcatggggag aacatggggt agacatcgta tgcggtgaag gacaaccttt
     9961 aggctcacct atggcatcag gaggccctta ttttggtttt ctgagcaccc gtatggccca
    10021 tgtcagacaa atgcccggaa gaataattgg ccgcacggta gacaaggatg gaaaaaccgg
    10081 ttttagttta actctccagg caagagaaca gcatattcgg cgtgctaaag ccacttcaaa
    10141 catatgcacc aaccagggtt tacttgtaac agcagcaact atctatatga gtcttttagg
    10201 accggaaggt ctaagccaag tggcaactca atgccatcaa aatactcatg aattgattac
    10261 tgccttaaca caaatcgagg gggtagaact ggcattcaaa gctccgtttt tccatgaagc
    10321 attgattaaa ctaaaccaac cagttcagtc cgtattgcaa caactagccg acgctggaat
    10381 tgctggaggt tacgcaccgg aacaacacta ccctcagcta gccaatacgc tgttagtttg
    10441 tgcgacagag gtgcgtactg cagaggatat cgcgaaatat gcaaaaacat taaaaaccat
    10501 aatgtccaag cgaggtgcct gatgtttatt gtgcaattaa cctatttagt tcccatcaat
    10561 gaagtaaaca aatatctgca ggcacacagg gagtttctag attaccacta caaacaaggg
    10621 ttattggttg cttctggccc catgaaacct ccaaccggcg ggattattat agcagcgaca
    10681 aatgacaggg cacatttaga gtcagttttt aaacaagacc cttattattt agcagaaatt
    10741 gctgagtatc aatttatcga gtttactcca gtaaaacaca ggaatgaact caaggaactt
    10801 attcaaaaaa cggaaggcaa attatgttga tttttgaatt atctaaaact ggtcgccagg
    10861 caaaagccca aatacccaga gcagtaagca aaaactattc aatacccgaa gaatttcagc
    10921 gaaagtcgcc cccaagattg ccggcctgct ccgaattgca agtggtaaga cactttacct
    10981 gcctttccca aaagaatttt tccatagata ctaattttta tcctttgggc tcatgtacca
    11041 tgaaatacaa tccccggggt gttcataaag cagcttcgct tcccggtttt ataaatcgcc
    11101 atccgttagc tatggacaat gaaagccagg gatttctgga aactctttat aaattacaaa
    11161 attatatttc tgaaattacg gggatgcccg gtgtttcact aactccaatg gcaggttcac
    11221 agggagaatt tgccggagtc gccatgatta aagcctatca ccagtctcgt ggcgacaccg
    11281 cccgagatga aattctcatt ccagatgcgg cgcatggaac aaaccctgcc tctgcggtaa
    11341 tgtgtggctt caaagtagta gaaatagcta ccgctccgga cggtgacatt gatcttgacg
    11401 aattaaaaag aaaagtaggt cccaggactg ctggaattat gctaactaat ccatccactt
    11461 tgggattatt tatgcgtcag ataaaagaaa tagccagtct tgtgcaccaa gcaggaggat
    11521 tattatacta cgatggggct aatctcaatg ctattttagg taaagtaaga cctggtgata
    11581 tgggttttga tgtcatgcat cttaatttac ataaaacatt tgctacacct cacgggggtg
    11641 gcggtcctgg cgctggccct gttgctgttg gcaaacgcct tattccttac atgcccttac
    11701 ctgttgtgaa aaaaaccgac tccggatatc actgggcaac tcgccaggat tatccacaaa
    11761 gcataggaag attatcatgt tttatgggta atgccggcat tttattacgt gcttattttt
    11821 atatgcttgt ccttggtaaa gaaggcctat tgcgcgtatc agagttcgca acacttaatg
    11881 ccaattattt acttaaagaa ttgactaaag tgggctatac agcagcctat cctgacaggc
    11941 gtgcatcgca tgaatttatt cttactttaa attccgaaaa gaaaaattat gacgtgactg
    12001 ccatggattt tgcaaaaagg ttattggact atggagttca tgcccccact acttatttcc
    12061 ctttactagt accggaatgc ttgttgattg aaccgcccga aacagaaagc aaagaggaat
    12121 tggatgcttt tgtggccgtg atgaaaatca ttcgcgaaga agctagcaaa caacctgata
    12181 tactcaaagc tgcgccccac actttaccag tcaaaagact ggatgatgtg aaagcggctc
    12241 gcgaactgga tttaaattat tttgcaactc acgagtaacc tgtacaatag atttttccaa
    12301 ttttagcgca gcagctttct ttttatgaca gttgctgcgt tttatgacgg ccaatctttt
    12361 ctcgccaaaa atctgatgct cttttataac aattcacaag cttccgcatt ccaagactct
    12421 caagcatcgc attattaact ttacaaaata atgttataat ctcattccaa tggatcaaat
    12481 aaatggttgt agaattggat gagacaccag tcagaacgtt gctagcgcgt cagggaatct
    12541 atgataagaa tagcgcaatc gttgcctatg aactcttgta tcgtaatagt gatgcagaaa
    12601 actcacacgt agataaccta aaccagtcgt caggtgaggc agctacctcc tctgttctag
    12661 tacagttatt tgccaatctt gatgtaaata ccataatagg taataaacaa gcttttatta
    12721 atttcactca tagccatctt gtacaaaaaa tcccaatgtt gcttcctaaa aatagaatag
    12781 tcattgaagt tcttgaaacg gtggcgatcg accagccatt gctgctcaat ttaatggaat
    12841 taaagaagca gggatatcaa attgccctgg atgattttgt cttcaggaat gaattggctc
    12901 ctctggttga tatggccgac attattaaaa tcgatgtgct tcatcttgac cagcagcaaa
    12961 tcgcagaaca attattaccg ctgaaatctt ttagaggtaa gttattggca gaaaaaattg
    13021 aggacaaaca acaattcaat cattgcgtca atcttggatt tgattttttt caaggttttt
    13081 ttctaaacaa accggattcc ttaacaggac aaatcattac tgaaaataaa atacagcttt
    13141 taagattatt aacagaaatc aacaatgaag aggtaccaat ccaacgcatt gaagaaatta
    13201 tcttacaaat tcccaaactt agctatcgta tcttgcgttt ggcaaattct gcctctttgt
    13261 acatgagcaa aaaaatagaa tcattgatgg atgccatttc tcaattggga ttaatgcaaa
    13321 tacgcaactg gttaaatctg ttactcttgg caagtctcga tgatgtcgca cctgatttgt
    13381 tggagcgaac attaattcgc gccaaaatgt gtgaatcctt agcaaaatca atggattatc
    13441 ctaactctca tcaagcttat accgtaggca tactttctac tttagatggt attttaaatg
    13501 agcctatgcc ttcgctatta gcaaaaattc ggttaagtga aacactcaat gaagcgctac
    13561 tgaactacaa aggggattta ggcaaaatcc tgaaatttgc tatagattat gaacaagcaa
    13621 attttaatca attagaacgc attcctgtaa aaagcgaaac gctctctcaa tcttatctta
    13681 agggaataga atacgccaat catgtgatag acattattaa taaataattg tcgtccctat
    13741 tagtattcat tctgtattca acctgtttta caaaagagag tcttttggaa attgtccgta
    13801 tcagataaga caaaaaggat tttcatcagg acttacgaca aatcccaatc tctttgttaa
    13861 aaaaagtttt taattcggtc atctagttta tctaaactcc ctcattaaaa acattttttg
    13921 cctcgacctt ggggtttttc gtaagtcctg ttcatgtgac gaatttgcca ctaaatttta
    13981 tttaaaatca caaagacctt aaattgtcct tatagatcaa ataatagctt gatgtttcct
    14041 attgtttatt tataattcat ggaatgttaa atttattctc ttatttcaaa ttatgatcat
    14101 acgtgaactg tttaattgga tacacaatga tagcgcaccc cttcatctaa gtgatcaaac
    14161 gatcgaacgt gatcttattg aacaagagga atcacaagcc aaacaaactc atcgtgtgct
    14221 tctgggtaat gtccgtcttt taaaatatag tggttcatcc aatacagagg cgattaaaga
    14281 attagagcag cattgtcttt ttatggatta tctccagttg tataaacaag agtttgttaa
    14341 ggatgggaaa aacactgttt ttttaattgc attaagaaac ctgttatctc actctcatga
    14401 agagcaactt cgtctcatgc ctaaaattaa tgagttattc caaatattat tacgagacaa
    14461 taaagaaagt gctctatctt tttatgataa aaataaggaa ttattcaggc actgtacaga
    14521 aattcaagaa agggtaacat ttgagctctt ccagcaaaaa atagaggctg acagtaaatc
    14581 agtaatgact cagctagact atttcaatga acatttagcc taccaggaaa atcccttagg
    14641 aatcattgcg ctatgccgaa attggattgg tgaaacagaa aaattcactg ctttaatact
    14701 ctggttgcta gaacgcaaag taagcgttga aaaaattctt cttactaatc tgctgcagga
    14761 ttttcttaga tatcatttat tcactttgca ttccagggat aatgaagttt ctcgcttata
    14821 ctccctgctc ggccatttcc ctgaagctca ggaactcgtt atggcagccc aaaagataag
    14881 ctgtggagaa ttaatgttcc agcaatactc tcttgacggc acttttcggg gtaaaaatct
    14941 tcctgttgtt tcaccagaaa tccccccttt gcaattttcc ttaagcaaag acaattttat
    15001 cgcattacac cgaacttttg gcccggcttt tttaactgca ggaattgcta ctgcaacaaa
    15061 ccatgacgaa gtagcttggc tggatatgtt aaggcatact ctgaatcagc cagagacact
    15121 caaattactg cccgatatta tcaatattat tgccagagaa tattcaccga aagtcttaaa
    15181 aacccttgcc gaactcattg cggagtccac tgcccatcaa cttcttactc ttaaccaaag
    15241 ctgtgttttc catttactac aacataaacc aagattgctt cacgatatta cagaagaaaa
    15301 tgttattggg tatatcaatc atctggttcg actggatact catgatcagg aaatcattta
    15361 tcagttaatg gctttgttta gggtgttatt gaagaaaaac catccggcaa cgaaagcagt
    15421 attcgaagca attctcgata atcttgtaaa tcatccgcaa ctgctggagg atgaagaatt
    15481 tttaacacag ttaaaaaaat atccagattg tgagctcctt ttagacgaac gttgtgaaaa
    15541 tatcttaaaa cagcttaatt tttgcattgc tgaacaaaca tcaggactac tctttggcaa
    15601 acataactac ttcatcatag aagatgtttg gcttggagcc ttaagaaaat ttgcagtact
    15661 ccagcaaatc aacccacaga tgaagtttag ctttgggcat aagtatgccc ttcaagcaag
    15721 aattgccgaa gttgtcttta tccatcaggg agacttgttt gatttagata cctttatcga
    15781 cgcgttggat ttgcccctcg tcacctcatc aggagaaatc agtccttacg aacgggctct
    15841 tatcgaaatt cttgccacga tagataatga gctcatcaga aaacaaatca ttcaaaagct
    15901 ggaaacaacc cctttcaata gacttgactg gcatgaaagg gaatatggga atcaaactgt
    15961 tttcattaaa gcagcaaaaa agggtaattt aggcttaatc aatttgcttg aagacaagat
    16021 ggaaccttct gtgcttaaca aagcgctaag agcagcagcc aaaaactacc aatgggaaac
    16081 attggatcat ctttgtagcc ttcctggtgt tgagttaagg caagatgaaa tagatgatct
    16141 tgtggtctat ctggcagaac atgggcgaat agaaagtgta aaaaaactct tgaaattata
    16201 tgattacaag ccatccacag aattaattgg cacgattctt aaaaaagcga tagacaatga
    16261 taatttacaa gtcgtcatgt atttctgtaa actgccagtg gaatcaccca aacaatcaat
    16321 gcttgatcgc ctatttaaat tggcaatcca attacagcat tgggacattg ttgagtatct
    16381 tgccaactca aagcattatt ctccatccca aacgacgctt gaaaaggctt ttcaacaaac
    16441 tgcgctggct atgcaacatg aagctgtaga aattcttggc aatgtggaaa agaatcccgt
    16501 acgtgcaata gttattgagc gggctttact gaaagccagt aaactggggc atacaaaggt
    16561 tgttcaatcg atttgtgcct taccttccga gtcattaacc aaacaagcta ttgaagacgc
    16621 tctagaacaa gcagcagccg aaggacattt ggacattgtc tcctgcctct gtgagccagg
    16681 aaccacaacg ctaaggcaac cagggatcaa ttcaggaatg aaaattgcgg tccagacagg
    16741 caaattatct gtggttaatt atttctgctc gatgacaggg agcaataaac caactccccg
    16801 cttaattgat cagacattag tgatggcggc taaaaaggga caaacagcca tttttatggc
    16861 tattcattca aaccgccaga ctcctccagg aaaacatgct attgagcaat cctttcaact
    16921 tgctatcact gcagggaaat tacaaatttt agactattta tgtcgtcatg aaagttatgg
    16981 cctcaatcaa agcaggatag atcaagcatt gatttcagca gtcaaatcca aacaactggg
    17041 aattgttagc tatctttgtg aatctctgga aattacaccc agtcgaaaag cactaaaaat
    17101 tgctgtttct aaagcaatct ccagtgacca aactgaatta gctgattact tgcgaagccg
    17161 atccagtggc aaatcaaaac ccttagatac acccactgac gagatggctt cccctctcga
    17221 aattggtaaa caattagcga ctaatgggct ttttaaatat aaaagaaaag acgccaagga
    17281 gcctccactg cttttaaatc ctagtcttta aaactattgg tcagtagagg aaactctctt
    17341 tctttaattt tatttcgttt aaccttttgt ataaccgatt atttttgccc gaaatgatat
    17401 attattgacc gacatccaca aacaataacc atattggctt gggagtcact aagccatgtg
    17461 cactgccgtt caatttgtct gactcaatca ctggttataa agagaaaatg acgtcatggg
    17521 taaaccaggt caggcaattg ctcagccaat tgcaagtata attttgttta aaaccgtcca
    17581 gaaatttggg cagtttgcac caagtatccc taatcatcat acttggacat ccagctcgaa
    17641 caaagtcaga gaatccatct caccatcgta aattttctta aaccaagctg gtatttgttt
    17701 tgacgattca gaaatatatc gtgacctctc tttgcactgt tctggaagat aactgttaaa
    17761 taatttttct aaagcgcggt tatcatcaat taacagattt atttggttaa ccagataata
    17821 agtttctcgg ggtttaattt tttgtcggtg accatactta tcaagccatt tcaatgaagc
    17881 caaaccaata aataaagacc atgcacaaaa taagcgatac gggcgtcctt caataattgg
    17941 gatgctcttt acataagcca gtgaatggtg tgcatgaata accaaactct cgcgcaagct
    18001 attctctgaa gaaataaaat aacgcccact tgtttcatca tccactctat ctttcaagag
    18061 attgattttt tgtaaaaaga aaccaaagtg aaaggcttga tttaacagac tggctgtcca
    18121 cttaaaatta gatagtaaat cagtaaatat gtgtgacaat aatttgccaa ctaatcctgc
    18181 aacaaaaaaa cagtattgat tagtggtaac taatgaagga aatacgattt tgccttccat
    18241 atgataatga ttaaggaaat aatgcattcc atccactact tgcaaaattg tttttatcac
    18301 agattgcttg atggattcag gtaaattatc tttttcttcc aacaaaagag ggacggcaca
    18361 tagaagttgt ttctccccta atggaagctg cgaaggaaag ctagaaagcc attttgtgaa
    18421 ttgatcgttt gccggtgttt gaactaaaaa tgatttaaaa gcagcaaatg aatcactttg
    18481 tgtttgaaga tcttgccatt tggcatcttc aatactgtct gcaacacgca aaagtaaata
    18541 actcaacgca acccattctt ttgctggata agttaactgg cttatacaaa aactaaaact
    18601 tctgcttaca ttttctagat gttggtaata aaaagaaaac gatcgcataa ccatcagcca
    18661 agttgatgtt cgcttttcga taacgcctgt tttattctct cgtaattttc ttccactgtt
    18721 tttgacaact catcatatga aatataattt ttccatacat gcattaaaga aaataaatac
    18781 agaaacccaa actccaggtc gttttccaat aatgtattac tgtacttgat aaagctatct
    18841 aaatttgaaa agggttttga cagtggcttt atcctggatt gctccatgtc ttgaaacatt
    18901 tgatcaaaag cctcctgcat ataacgcggg aacgggatat tgccatcaaa aactcggtga
    18961 tcaacactca tgcctacagg caaaacatct tgcacctcaa atgtgttatt tgttttattc
    19021 cagacttgtt ttctttcgat ttctgtcaga gttattttaa acgtttcatt tggaaataag
    19081 ggtgaaaccg ctgcttgata tccccaatga ccaatattgc ttaaagaaat agcaggtctg
    19141 gcaaataaga aatcacggta cactcgctcg tgccgtggag ttagcaagcg attgactacc
    19201 tcgattaaat agggatgctc tttttgtaat gattgtgttt tttcataaca atacttcatg
    19261 atgcgcatgt catgctgaat gtgttgcgct aattcagtca tagtcatctc atggcagttt
    19321 ttaaactcaa tcgcacctaa ttgatcacta cctggaatct ttaccgcaag cgctacgtaa
    19381 ctattgtgcg agtggtacag tttatgattg aaaatataat ttctcgtctg cggattatca
    19441 aagcaagctt tactcaatgc ttgtagcatg aggcaagtta ttgtttccgg tttaaatttc
    19501 ttttcaaaaa aacaaacagg agtaacattt atattaaaaa tagcagactc agttggtaat
    19561 tcggggtcat tccaaattgc tgaaagtaat ttacgaatat tggtcggttc aatggctttg
    19621 tctttttcaa aatcatgcga taaattcttc atcaataaag gcatggtatc cataaacaat
    19681 tgccaatggg ctgaaggcgt taagccaatt tttttaagca tccaatggtg tttattattg
    19741 atattattga gcagtttcag tgctccctcc ttgcctaata gaataccaag ggtagtcaaa
    19801 tattgctcat gcgcaaatac ggtatttatc agctcatctt caaaaattgc taattttttc
    19861 cttaaatagt ctttatttgg caacccaatc tgacgatata aatcgtactc gtccatatgc
    19921 cggctttcat cttcaaggac tgtttcaaaa atagttactg cgatgttttt ctctttcatt
    19981 gcaatgcata attcttctac ccatccttct ccaaccaaat tgaccaaggt gatagaagtg
    20041 gccagatcct cttcgccttc aagagaagaa ataaaatgat tgatactctt ttgatgacca
    20101 agtggtaatg cgtaaggagc ggacaactga aacgcaactt ttgcaaaaac agtggcatgg
    20161 aaaatctcgt cggtaatttg ttgacgcatt tgttgataca aatctgttcc acggagtttc
    20221 tctagagatt ttataagccc tcttaggcca attgtttcta attgcgcaaa caaggaaaat
    20281 aaatgaatat agataagctt gtcatgctca agttgaaaag gaatttcgtt gtaaaatatc
    20341 ttgtccattc ggcttttgat ggtttccttt tcctcgtcat tcaatacaga ccaagcatta
    20401 tttaaagacc gctccgcagc ccaagttggt tttaacaaat cgcctaactg aatcataaat
    20461 taaccccaat ccatttgtta tattgcaaac gcctacactc ttccggagca catacgaata
    20521 tttaagggca agtgtactat tgaaagcgtt tttgtggata ttgtcatata ctgattgtaa
    20581 ttgctactgg atcccagcct gaaaataacc atagataaaa aatgcgatgt ccatcagata
    20641 aaaaaagact tcttaaaatt atcttatcga tagcgataaa ccccctttgc tcaatgagtt
    20701 attcacaaat ctcgagagtc tgagatgact ggttttcggc tcactccgac ttctcaacag
    20761 ggtagacaaa gtacgacctg atatctcata caaaccaaag tacgttagct gattgacgag
    20821 tcatttaaaa tcaaatctct atagtaaaat aagcatcccg gaactgtttg caatgcagca
    20881 atataacaaa agggactgat tagagagagg aactattatc tcaatggaaa ataattgatt
    20941 ttaatagcaa aaatatcttt gctattaaaa aaaaataagt tataacactc ttaactacat
    21001 caaggattga ttagtgcaaa tgttgggtca acatctcagt aaattcatgt tgagttgact
    21061 gtataatttt catggagtga aaaatgaata gtaaagtatt ttcgcaacgt tttaatcgtg
    21121 aattggcaac tctgggattc cccgaggatt taacagaaaa aacgaaagcc gtatcaaaag
    21181 tatttggtgt cacacgccat ttggctaatg caatgatatt tggccacgtt cttcctgata
    21241 aagaacaact ggataaaatc gcagaaattc tggaagtttg tcctcaatgg ctaagcgggg
    21301 caacggatag gaaaaaagcc tactcaggcc gtgagactgc tgatagtgtg taataaacct
    21361 ggaatttcat gtatactgta agctaataga cagcgtttgc taaaattatt tactttgcaa
    21421 acgctttttt cattgttggg ttattaaaat catggaatgc ttaagtttct gtgtggccaa
    21481 aaccatagac ttgactcgtc ttgatcttca tctaaaaaac gtctctaagg aatttagtgc
    21541 tgtaaaaacc cgagatgtaa taagattaaa ttctcataga aacaaagatc acaccctgtt
    21601 catttttaaa aatggaactg tcgtatcttg gggtgtaaaa agataccaaa tccacgaata
    21661 cctggatatt attaaactgc ttgtagacaa acctgtagca cttttggtgc atgatgaatt
    21721 tcattatcaa attggtgata aaacagccat agaaccacat ggtttttatg atgtggattg
    21781 cctgaccatt gaagaagaca gtgatgaact aaagctcagt ttgtcttacg gcttttctca
    21841 atctgtaaaa ttacagtatt tcgaaaccat tattgacgca ttgattgaaa aatataatcc
    21901 gctgattcag gccttatctc ataaaggaga aatgcctata agcagaaaac aaattcaaca
    21961 agttattggt gaaattttag gtgccaaaag cgagttgaat ttgattagta atttcttata
    22021 ccaccccaaa tatttttggc aacaccccac tctagaagag catttttcca tgcttgagcg
    22081 ctatttacat atacaaagac gcgtcaatgc cattaaccat cgtcttgata cgcttaatga
    22141 aattttcgac atgttcaatg gttatttgga aagtcgccat ggccatcact tggaaataat
    22201 tattattgta ttaatcgcag tagaaattat aattgcggtt atgaatttcc atttttaggg
    22261 tttttccctt aatttatgcc cactctaaaa tcctattttc tcaaagacac ctctttgcgc
    22321 acggaaatac ttgctggagc aacgaccttc cttaccatgg tttatatcgt ttttgtcaat
    22381 ccaaaggtcc tgcacgatgc aggaatggat caaggcgcgg tatttaccgc cacttgtttg
    22441 ataaccgctt tcgcctgcgc attaacaggc ttcatagcca acacaccgat aggtgtagcg
    22501 ccaagtgtgg ctttaaatat ttatttctct tacagcgtag tacaagggat gggagttgac
    22561 tggcaacacg ccttggccat ggtattcatt tccggcttgc ttttcctgtt tcttaccctc
    22621 accaaattaa gacgcctctt ggttgaagca atccctcata atttacaaat agccattgtt
    22681 ataggcatca gtttgctaat cgccttaatt gctttagaaa ataatcaaat cattattggt
    22741 gaccagcaca atttaatgcg attaggatct atcatcagac cagaaacagg actctttttc
    22801 ctggggttta ttcttattct tactctggat tatcataaga ttccaggtgc catcatcatt
    22861 gggatactga gcatcagtgt cttaagcttg atattgggct tgaccagttg gcaaggtttg
    22921 attgctatgc cgccgtcgct tgaacctacg tttttgcagc tcgatttttc tggtctttcc
    22981 agtatcgtgg ctttaaaaac catttttacc ttttttctga tcaccgtgtt tgatgcgaca
    23041 ggatcattaa ttggcttact aagtccaacc atttttaaac atgaaaaaaa ttattataaa
    23101 cgcctaactc gcagtttaac tgctgatgca gccgcatctt ccctcgcagg cctaatgggc
    23161 tcagccagta cctcacctta tattgagtca gcaaccggaa ttgaagccgg tgggagaacc
    23221 gggctaactg cgttagtcat tgcaactgga tttattctga tgctgtttct ttttccactt
    23281 gccaaaatga ttccaagctt tgcggttgga ccggctttac tctacgtagc gtgttgcatg
    23341 ataaaacata ttactaatat gaaattacag gatatcaccg aaacagcgcc ttgcatggtc
    23401 atcatcatga tggtgccatt caccgcttcg attgccgatg gaataggttt gggaattata
    23461 ctttataccc tccttaaatt atttactcgt caaaaaccca atcctttgat tattgtttta
    23521 agtcttgttt ttaccgtttt ctttctgatt aactaagtga gtgtgatctt taaatccagg
    23581 ataagccgta aaacctccat aaaaaacttg tctgctctca aaaactgcaa acaaaagttt
    23641 ctttaattta ttgaatttag tagtatttaa ataagtgaat tatgcacaag ataggtcgtt
    23701 atcttaaatc gttcgtttaa tttttcgtgc caaagcttcg agatgatact taggcatcgg
    23761 atatcgaatg cttcttatat agtacttgtc cataactcac gctagatagc caaataactg
    23821 ccataagctc atagttggcc taatcgcgat gaattattat gcttgaacaa tcattacgac
    23881 attattatca gcttgtcttg gtagatccca tactacctgc tttacgtgac aggataacgc
    23941 cattgtccat tacctggttg tcgggaatat ttggcttgtt gtttattcct tttgtcttat
    24001 tagacaaacc ttatattgca attagctttc tcttgctttc tggatacctg gatactcttg
    24061 atggttcgtt ggcccgcttt caaaacagag cctctgattt tggctcagca atggatatcg
    24121 tcatagatag aattgtagaa ttttctgctg ttcttgcttt gtatcttttt gatccacaac
    24181 accgtgccac tttagccatt ttaatgttag gcagtatttt gctctgtatt acaagttttc
    24241 tggttgtggg aattttttcc aacaatacta ctcaaaaaag tttttattac agcccgggga
    24301 taatggagcg tgccgaagca tttctattct ttatttttat gacattattt tccagctgct
    24361 ttaattttct tgccgctctc ttttgttttt tagtctgttt aaccgcgttg ataaggcttt
    24421 ttgaatttaa aaaaagtgta caggctccaa gttagcgaaa caataagttt tccgaagcct
    24481 gatttaaaac tttctctgat tttatgctga ctacaataca aaatcttgta taatttacag
    24541 caattcataa aagaggctta caatgttgcg tatccttttg tattttctct tactcattaa
    24601 tacaacattt tgtctggcaa agactgaaaa agccattttt gcaggtggat gtttttggtg
    24661 catggaagca gactttgacc atcttcctgg agtcctgtcc accacatcag gatttgatgg
    24721 gggcactctt aaagatccaa cttatgaaca agtttccgcc gggaataccc attacgccga
    24781 atcggtaatg gtggagtttg aacccaataa attaagttac aaaaaactgg ttgagtactt
    24841 ctttaggcat attgatccta ccgtcaaaaa tgcgcaattt tgtgatcatg gcagtcaata
    24901 tcgcagtgca attttttatc ttaacgatca acaaaagaaa attgccatgg aatacaaaaa
    24961 agagttagag caaaaattcc ccacagttta tacagaaatt gctccatcaa cttcttttta
    25021 tccggcagaa gaatatcatc aaaattatta caaaaaaaat cctatacgtt ataagtatta
    25081 tcgatatcgt tgtggccgag atacccgaat tcaagaggtt tggggtaata aaaaataaaa
    25141 gaataactct taatcatgat ccatagtttt atcgcttatc aaaaaagaat aagcaattca
    25201 tgagaaaaca cgctaatcta ataaataata atttttaatt ttttactaat acaattatga
    25261 ttactcaagt taaccaatta gatgaacttc aattaaaaga cttaaaaaca ttaagagcag
    25321 agtgtaaaaa aaatgatggc agcatcccca atctatatat ccatatattg aaacaacacc
    25381 gctctcttcc tacaagcttc ctttattatc aaaacggggc cttgattgga ttcttgagta
    25441 tttatttctt ttatgatgat gctgttgaag ttgctgttct ggttagtccc caatatcgca
    25501 gacaagggat tgccaaacaa ttaataaagg aagcattacc cttaattaag tctcaaaact
    25561 actttaactt gattttttca tgcccctcac gcctcaatga taactggtta acctcaaaag
    25621 gatttaccta cctgcatagc gaatatttca tggaacgtga tgatttaaat cctatactcg
    25681 attacatacg tccattgtcc tttcgcatgg cgactttaga ggatattccc atattatgtg
    25741 gcctcgatga agtatgtttt cctgataaaa accaagactc ggttcaccga ttccaacaaa
    25801 ttcttaacga aagagaatat gaaattgtaa tagccatgct caataatcat cccataggaa
    25861 aatcccatat tcgctggcaa accaaaaggg ctaccttgtc tgatattgcc atattgccta
    25921 aagaacaagg aaaaggattt ggttctgcct tgattgctca ttgcatcaat atgattttga
    25981 gcgaaggcaa atcccgggtg gaccttgacg ttgaaaccca taataaaaaa gcattaaatc
    26041 tgtatattca gcttggattt catattcaaa atgcttgtga ttactggtca attaatgtga
    26101 atcaacttgc aaaataaaac tgtttattct tttgttacaa attacatatt ataaatatgg
    26161 cttgacataa ttaaaatctc accattagta ttgcgaatgg cgatagagga aggcttgttt
    26221 tatcaaaaat aaggataatt aatgaaaaaa agggttgtta ttactggtat ggaaataacg
    26281 tcatccatag gatcgggaat tgataaattt tggcttgctg catctcaagg tcaatgcgga
    26341 attaaacgca tccaatctta tgatccatcc ccctattcga cccaaatagc aggtgagatt
    26401 acagacttat ctctcgacca tctccccgaa ttcaacaaaa gcaaacgtta tcctcgcgct
    26461 gctcagtatg cattattttg cgcacatcat gccatcgaga aagcagggct tgagcaagct
    26521 gagctcaatg cagcaggcac ctttataggc acaagcattg gcggtcaacc tgaactggaa
    26581 gcaggttaca ggttattctt tacagacagc tggaagaaaa tcccccctct tagcgtgatt
    26641 agggggatgc ccaattcaat cgccaatcat atcgcaattg cttttggcat aggtggacca
    26701 aactcaacca tttccaatgc ctgtgtttca tccggggaag cgataggcat ggcttaccaa
    26761 caaattgccc atggcaattt gaccacagct ctttgcggag gaacagaatc tttagtctgg
    26821 gaatcggtta tggctgcctg gtgtaagctt agagtaatgt caactaacaa tgaaaatccc
    26881 tcacaagcca gcagaccgtt tgatctcaat cgggatggaa tggttatggc ggatggggct
    26941 gggatattaa ttcttgaaga gctacaacaa gcaaaagcaa ggggagcaca aattctcgga
    27001 gaaatcattg gctatggcgc aagttgtgat gcattccatg tcacagcgcc aaactcacaa
    27061 ggacaagcaa gagctattgt tgctgcttta aatgatgcca aattatcaat taacgatgtc
    27121 cattacatca acgcacatgg gacaggaacc cagctcaatg acagcacaga aacagaaacg
    27181 attaaaatgg ttttcggtaa aagagcctat gacatcccga ttactgctca aaaagccatg
    27241 acaggacatg cgattggggc agcaggagca atggaaatca ttgcaacaac actaagcttg
    27301 caaaaaaaca cgctgttacc aactattaac ctgcataccc cagacccagc ctgtgatctg
    27361 gattatgtgc ctaatgctgc gcgagaaaaa accattgata ttgctttatc caaccatttc
    27421 gctttcggtg gagcaaatgc cgctttagtt ctgcgcaaat acattagtta agaatataaa
    27481 tagcttacgc taaacagcaa ttgattatta tataaatggg tcaatcgtgg ccctttggta
    27541 atattaactc catcaccacc caaataactt ttccctaagt ctgcgaactc atatcctaca
    27601 ccgacctgcc agttacgatt taacattttt tgaacaccgg cccccacact gtaagcaaaa
    27661 gcataagtgg tgtttgacgc aaaccatggt gcaggaaaca ggaattgatt gatagaaaga
    27721 gtcctgtaat catgggcatt attaaatgct attccaaaac taccgctcag ataaggttgc
    27781 acgggtgtaa atgcacttcc tatcagtttg ccttttagct ctactcgtgc gtgattcact
    27841 ttatagtcat acgtatagac attaaggagg ccattcaggt tgacatcacc agtaaccttg
    27901 gcatcgctta ctcccgctac ccctaaaccc aattgaccaa tcatttgggg attgatcgca
    27961 cgctgcagcc caaagaatat ctcgccattg gctaaaatag tcgtcggaga atcatgggta
    28021 aaataaagat aataatttgg cggtaaattg ggattgaaat tattaatata gactggagac
    28081 attggataaa tatactgatt ttgtccaggt gttgcccaag aaggtcctcc actcactgta
    28141 ataatagagg accaaagtaa gggaatttct tcgtcataaa tgggttctga agcggaaaga
    28201 ttcatactaa acaaaacaca taatccggtg agcaatattt ttttcagcat atcccatcct
    28261 tttgattaca aatcagccct cttgattcag gctctttaat gaactgattt taatagaagt
    28321 tatttgattt tataccctaa ttaatctcat ttttctaggg tgcgttgaca attcatctgg
    28381 ctcaccagaa tagcaggatc atgataagaa aaattattaa tctgaaagcc atctgaatcg
    28441 aggataattc actcgaaaat ggaccataag aggtatataa tcgctctatt ttgcggaatt
    28501 gaaaggtaaa ccaatgaatt cttctcttcg tgatatagca aaccttgttc aaggtatggt
    28561 tattggcaat gatgccatca ctgtttccac actatcaccc atagataata tacttcccgg
    28621 atctttagtt ttcgccgaag gtgaagacaa tatcaaactt gctgaaaact ctgaagcagc
    28681 agctattctt attggtcaag gtactatcga ttccccaaag ccattaattc aggtaaaaaa
    28741 cccttttaaa gcatttattg ccttactcaa ccatttttat cccccaagga gaatatctcc
    28801 gggggtacac cccactgctg taataggtgc agaagtacaa ttaggtgatg aagtttatgt
    28861 tgggccattc gtagtcattg aatcaggaag tattataggc aaccactcgg tgctcaaaag
    28921 ccatattcat atcggccata atgtggtcat tggagatcac acaaccattc acccccaagt
    28981 cactatttat gataattgcc gtatcggttc gaatgtcact atccatgcct ctacagtaat
    29041 aggttctgat ggctttgggt atacttttgt tgatggccaa catctcaaag tacctcattc
    29101 gggatatgtg gtaattgaaa ataatgtcga aattggagcc aacacagcga tagataaggc
    29161 cacattaggt gctacagtga taggtgaagg tacaaaaatt gataatttgg tacaaatcgc
    29221 tcactcagtc aaattaggca agcataatat tatttgcgct tttactggca ttgccggcag
    29281 taccactacc ggaaacaatg tcatctttgc agccaacgtg ggagtgagtg accatgttca
    29341 catcgaggat gaggtcatat taggcgcacg tacaggagtt cctccacaca aacacttaaa
    29401 aaaagggacg gtatatcttg gtaatccggc aaaacctaaa gatgttgcga ttaagcacga
    29461 actgtcagtg aatcgtatcc cattaatacg aaaaaacatt aaatcactta ccgagcaagt
    29521 ggctgtaatt aacaaaaaac ttgatattaa agcaaaagag gtagagtaac tatgaaaccg
    29581 cctctgattc taatagacgg atcttcctat ttctttagag cgtttcatgc ccttccacca
    29641 ttgacaaact ccaaaggcca gcctacaggc gccatttatg gagttgccaa tatgattaaa
    29701 aaaataatca aagattatca accagaagaa atagccgttg tctttgatgc caaagggaaa
    29761 acattccgag atgaatggta tccagaatac aaggcacaca gaccaccaat gcctcaagaa
    29821 ttaagctctc agtttcaacc gctaatccag ttactgcaag caatgggatt gcccttgctt
    29881 atcatcgatg gagtcgaagc agatgatgtg attggcactt tggcaaaaca agccacagag
    29941 caaggcatac cagttgttat atcaaccggt gataaagaca tggcacaatt ggtcaatgaa
    30001 catgtctccc tcattaatac aatgagtaat tacagtatgg atattgatgg tgtcaaagca
    30061 aaatttggtg taacacctga gcaaatcatt gattacctca ctctcattgg agatagtgta
    30121 gataatattc cgggagtaga gaaatgcggt cccaaaacag ctgtaaaatg gctgacagag
    30181 tatcaaacgc ttgataattt gcttgcacac gccaacgaaa ttggcggcaa aattggtgaa
    30241 tatttgcgtg ccagcatccc tcatttgcca ctctcaaaaa aattggtcac tatcaaaact
    30301 gatttggatt tgcccttaaa tttaaaccaa ttgatgccca aaccggctga tagcgagcaa
    30361 ttaattaaat taactcgtga attagaattt aagtcatggc ttaaagagtt actacaggaa
    30421 gaagagaatc aaaaatcacc caaagaaatg gatgccagca ataataaatc ctatgaaata
    30481 ataacgactc ataaacagtt aaatcattgg cttaataagc tagagcaaag tcaacaattt
    30541 tgcatcgaca cggaaacaac caaccttgat gtcatgcaag ctgaattggt aggcatctct
    30601 ttagcagttg aggaagaaaa tgcatcttat attccccttg ctcatactga tggcagtaca
    30661 cagttagtaa aagaagaagt tttaactgct ttaaagccta ttttggaaaa tccagctata
    30721 ggaaaaatag ggcaaaacat taaatatgat tacagtgtcc ttaaaaatta tggcattacc
    30781 ttaaaaggca ttgggtacga caccatgctg gaatcctatg tgctcaatag tggtgcaggc
    30841 agacatgata tggattctct agctttaaaa tatctgggtt ataaaacaat aagttatgag
    30901 gatgttgcgg gaaagggagc caaacagctc cgttttgacc aaattccagt agtgaaggcc
    30961 gggatctatg cagccgaaga cgctgacatt actttgcgat tacaccataa gctgtacccc
    31021 atgctggatg agtcattgcg taacgtgttg catgatatcg agataccttt actcactgtt
    31081 ctcgcagaca tggaaatgca cggcgttctc atcgacccag tcattctgga aaagcacgga
    31141 agtcgtttga aagaacaaat gaaatctctg gaacaggaag cagttcaact cgctggaaaa
    31201 gcattcaatc tcaattcccc aaaacaatta caagaaatac tatttgatga acaaaaactg
    31261 cctgttgtag cgaaaacccc cacgggacag ccttcgaccg cagaatctgt tttacaggaa
    31321 ttggcttttg attatcgcct tcctgctgta attctggaat accgaagctt atcaaaattg
    31381 gtcagtacct atattgatgc cttacccaaa agaatcaacc ccaaaacaca tagagtgcac
    31441 acatcctata atcaagcggt agcagcaaca ggccgattat cttccagtga tcctaactta
    31501 caaaatatcc ctatacggag tgaagaagga aggttgattc gaactgcctt catagcacca
    31561 gaaggaagtc ttatcatggc ggctgattac tcgcaaatcg agttaaggat aatggcgcat
    31621 ttatctcaag atgataacct tttacgagct tttgctaatg gctgggacat ccatgccgca
    31681 acggcgagtg aaatctttca aacgaatttg gatgctgtgt ccaaagaaca acgaaggcgt
    31741 gccaaggcag tgaactttgg tttgatatat ggtatgtctg cctttggact ggcaaaacaa
    31801 attggtgtgg agcgtcagga tgctcaacat tatattgata cttacttccg ccgttatcct
    31861 aaggtacttg aatacatgga gcgtactcgc aaacaagcgc atcaacttgg ttatgttgaa
    31921 accttgtttg gccgtcgtct ctacttgcct gaaattaatt cacgcaacct aatgagacaa
    31981 aaagcagctg agcgcactgc catcaacggg ccaatgcaag gaaccgcagc tgacattata
    32041 aaaaaaacca tgctggctat ttccagttgg gagcattcgc aaaagaaccc ctctgcaaag
    32101 atgatcatgc aggtacatga tgaattggtt tttgaagtcc gaaaggaagc ggttgaagaa
    32161 tgtcgcgcca ttatccacaa gctcatggaa caaactgtaa aattatctgt acctcttgtt
    32221 gtgtctattg gcacaggttc taactgggat gatgcgcatt gaagcgcata aaagcgttta
    32281 atttattgga tttaataata attaatagaa gtttgactcg caaaggaagg attttgacca
    32341 cctataaact caccaaaata ataagaccac gttttatgaa tacgttccaa accaatgact
    32401 ttgggccaag tattatactc attcaaaaac aggccatgac tcttaaacac tttaataaag
    32461 ttttctcgca tagcatcgga atcagaccag gctacaatag gaagttgcgg gtatttttgc
    32521 aataaaattc ctgcaagctc gggaccgttg cagttcattt catcagggct ggcacccaga
    32581 tcaccatcaa caatgacgat atcggggcaa tagttattga caaaggataa tgcggcttga
    32641 ctattattga caatagtcac tgaaattgat gcaacaaccg actccaacaa tcggcgcaaa
    32701 caaaaggcat taaaagcact gtcctcaaca attaatacac gcatcactgc tccctcttaa
    32761 ctacgagaga agtataatgt gcgaacctta agaaaatctt aagaaataga attaatccag
    32821 ttaaaaatta caaattgaaa aatcaacgaa gttccctatt aaattttttt tacaaattgt
    32881 gattttaatt tcatggcgcc tatcccgtcg atcctgcagt caatgtcatg atcgccgtca
    32941 accagacgga tattttttac tttagttcct acctttatca cttgagaaga gccctttatt
    33001 tttaaatcct tgacgaccgt tatcgtatct ccattttgca acaggttacc attggcatct
    33061 ttgataattt tcgcagactc ttcgtcattg ttattactgt tttttggcca ttcatgcgta
    33121 cactcagggc aaattaatac gtcaccgtcc tcataacagt attcagaaag acatttagga
    33181 caattgggca attggttcat tttgttgaaa aaccctgaat taaattgatt acaaactatt
    33241 ttactctaat agcaagcatt ttaaaaattt ctgactacat tgatctgtaa ttgaacaatt
    33301 ctggaatggc cggataaata acgatttttt aatcattatt tatccaacga aatagaaatg
    33361 ctatgaatta tggagtggat tagtcaatac gtattcttct tggcattgct cttctttacc
    33421 tttcgtggga gttcttcttg atagaatgct tgggaaatga ccaaccggat gaatattgtt
    33481 tgtgctcagc aattgagaac cacccaaacc ccgcatagcc tcatggctgc tttgcttaac
    33541 agcaggaaca gcctcttcgc ttacggactc tgatgtaatt tctgttcctt cagcaactac
    33601 aaatccaata gcacttgata gtgcagcagt gccagtacca cttatagcac ctaaagtcaa
    33661 agctcctaaa agaccaacac ctgaaggagc aaaaactccg gtagcaacca atgtaacacc
    33721 taaagcaaag ccaaaaccaa agccaatgcc tgcaaaagcg cccataatct ttagggtacg
    33781 aaaatcagaa gacggaaccg gttggttaga taagacagta gccgtttctt cacgccggga
    33841 taaattatcc tcttgaagta aaacaggggt ctcttcaccg atagaaacct catccccttg
    33901 ctcatctggt aattgaacct cttcaggaga gttctcttta ggcgggaaca tttccctaac
    33961 atcttctaaa tattgagacc attcaaactc cccgggattt ttacgatacc cacgaatttc
    34021 ctcctcattg tatcctttac cttttactgc acgcagcatc tcagcatagt ctgtcgggct
    34081 atcatcaagg atataaaccc ggttggccct ctctgttggg tgattaagcc gggaattgac
    34141 atcattaaat ttataccccg actccagaat ggttaacttg ctgatttctt catcagaaaa
    34201 accttgatat ttgaaaactg ctttaatgta ttcagctcga tttttggtga caatattgac
    34261 agtcacatca tcatttttta gtacttcatg gaaaaacgcc actgcttcag gactcatcag
    34321 gaaatcaaca tcctgctggt catgatcaaa attttcgttg tattttccat attttgcttc
    34381 gaataaagcc tgagcttcaa ttggatcttt cattggagtg tttttgtaat cttgctctac
    34441 atccttttta tagccttgta acagtgattg ataaaactcg gtaaatacag tttcattacc
    34501 gctttttccg gttacagtac catcaaaatc ggtaaaaata ataactttag gcattgtatt
    34561 tccttactat ttctcttaat ttgaaatgac tcgatattgt acaaagttta aattaagaga
    34621 atattaagta tagaaaatta caaaattatc aggctacctt ccctatacaa attgcaatag
    34681 actttgctgg agatgaatag cactccccct cgtccgatcc cggtacctga ttaatagaaa
    34741 aaatcaaata tctatatcgt gagctaacaa tcgcctgttg gcacgaaaat aggtcattgg
    34801 tgaatgctcg agtaaatcgg ataatttttc catgtagata caggcgaatc tatcaacttg
    34861 gtaggcgaag tagctctctt ccgccccagc gcgaaaaact ctttcccact tgggattata
    34921 gaaactgttt tgttcttgta acaatcttga aatttgtaag tcaacagtgg atatttgcaa
    34981 ttgcaagtca tgaatttcct gatcatactg ttgagagctt tcgtcaatac ttcttgtgca
    35041 aagatcgacg tatttctgct ctaactcttt tttaatagcc atggcttcac caattttctt
    35101 ttcaattggc aaagccctta tttgggatgc aatttcttca cctaattctt caacaaccaa
    35161 agcagtacgc caattacaat cttttttcaa tcgtaatata tcaccgtaaa tatgatcccc
    35221 tatatagaga atttcatctc caccgacccc gagatcttca gtaaattttt tggcattacc
    35281 tccttggtat acaccaggaa ctattgggcc atggacatta gtcatcgtcc cgttttcagg
    35341 attaactgat aaaaatcgta agttatcata aaagaaacgg ggtttatttg ccaaggtgat
    35401 aacaaactca aataaacctt gccagtgctc ccctttatcc aggaatgggg acaaggcata
    35461 atccaacaac aatttagaat aagaatattc tgaattggtc agaataaata tctttttccc
    35521 atagcgaata aaatgcttca gcccttcaac aacttccttt tctcttatca catatttttt
    35581 caaattttta ataattatgt ttttcagagt accatccgag tgaactttat ccacacaata
    35641 ttgcacgtct tgtgctatgg cttgatagct tggcatttta tcgggattgg tatcttttaa
    35701 atcaaccagc tggccataca ggatgcaaaa tgctatagaa aaagaagtat caattgccat
    35761 gtaattcgga tcacccaggt caacataaat acttctgtaa atttttttct gatcgctaaa
    35821 actgatctgt ttcgtgccat ggtaactcaa tctaatggca ccataccggc tcagttttaa
    35881 aatattgcca ttcttactgt caatcaccaa gccacgaatg gcatcatcaa aattaaattt
    35941 gaatttttta atttcctcag gatagtgaaa gctttctgcc agtctttctt tgacgagatc
    36001 ataaactaag gattcaaaat ttttcgaatt atatcggatg agggtatggt ccatgtccaa
    36061 accaatcagt ttaatttttc tcatatttat aattcgattt acaaaaacct tgtgcgtatc
    36121 catttttgag gaacctataa aattattggt aattaatgtt ttgagatatg aacgtataca
    36181 agtcgactat tttaaattat tcacaccact tgctgaagcc actaacactt tatctgccaa
    36241 acgtttggca aacactccat tttcaacgac acccgggatt acattaagac tgtcttccaa
    36301 ggccataggt gttgagaaac tcagattaaa cacatccaga ataatattac cattatcagt
    36361 aacgaaacct tctctgtatt cagggtctcc ccctaattta acaatttgtc gtgcaacaaa
    36421 actacgggcc attggaatga cttccacagc aacgggaaat tcccctaaac gttttacaac
    36481 cttggattca tcgacaatac aaataaattg tgtggctaca ttcgctacaa ttttctctcg
    36541 agtcagagct cctccgcccc cctttatcat ttcaccgcgt tcattgactt catcagcgcc
    36601 atcaacatag ataggtaaat cctgtactga atttaaatca attacaggga taccttctgc
    36661 ccgaagcaat gcctcggttg cttttgaact ggctacacaa gcctctatct tatgtttgat
    36721 tgctgccagt tcttttataa aaaaattaac tgtagagcct gttccaacac ctataaccat
    36781 atcatcttca atataagcta tagctgcttt ggctgctttg atttttaatt cactcacgaa
    36841 attgccctct ttgcctaatt acttcataca atgaaacacc agtcgccact gacacattta
    36901 aactctctac acttcccatc ataggcaaag aaaacaatcc atcgcaatgc tcgcgggtca
    36961 atcttcttaa cccggttcct tctgctccca ttacaagagc gatgtttcca gaacaatcca
    37021 ttgcataaag taaattctct gcttcgccag ctgctccaaa gatccagacc ccttgttgtt
    37081 ttaagatgtc catagctctt gctaaattag ttactctcac caaaggaatt gattcagcag
    37141 cgccacaagc cactttactg accacgggag taatactcgc acttttatct ttaggaatca
    37201 caacaaaatc aacaccggtt gcatcagccg accgcaaaca agcgcctaaa ttatggggat
    37261 ctgtaattcc atccagaata agaatcagca caggcttttt acttttttct aataaagtaa
    37321 ccaaatcact ttcaccgtat tcggctaatg ccgaagcgag tgcaacaacg ccctgatgcg
    37381 taaactctga aaactgctga ttcatttttt gcgcgcttag cttttcgatg gggacatggg
    37441 cgttctctgc cttgaccaaa aactcatgaa gtcttttatc tattctatct tgattaacaa
    37501 agagtttttt gatcattcga tgcgggtttg ctaataatgc agaaactgca tgcaacccgt
    37561 aaacatactg ctcattcatt acaaacttca tcctcgacag gctcaaaatc tattttacgt
    37621 tcgtttaaat caacacgagc taccaacaca gtcattttat ctcccaaacg ataaacctga
    37681 ccgccgcgag cccctattaa acgatgtttt gcagagtcaa acgtataata atcgtttttt
    37741 aaagaagtga catgaaccaa gccttctaca taaatttcat caagttccac aaaaatacca
    37801 aagctggtca cagcggatat tcgaccctta aatacctgcc ccaatttgtc ttgcatatac
    37861 tcgcacttta accaggtaat cacttcccgg gtcgcttcat cagcacggcg ctccgtcatc
    37921 gaggaatgtt tacccaaacg attcatatct tcatggctat attcaaactc ataaacagga
    37981 tgattatcca ataaatgccc tattgctctg tgaattaata gatcaggaaa tcttctgatt
    38041 ggtgatgtaa aatgcgtgta agcggaatag gccaaaccaa aatgaccttc attcgattca
    38101 acgtactgag cctgttttaa agaacgcagc attaccgttt caataagatg cttctctggt
    38161 cggtcaccaa tcatgttcat ggtacgctga aaatccttgg gtccaggttt tctcccccct
    38221 cccaatgaca atcccagctc ccctaaaaac tgcctcaatg cagtaatttt atcctcttca
    38281 ggagccgcat gaactcggta taaagtagga atttcagcct tttctaaaaa tcttgctgtt
    38341 gcaacattcg ctgccagcat gcattcttca attaatttat gagcgtcatt acgtattaca
    38401 ggcacgatac attttatttt cttattttca tcaaattcga tacgagtttc cgtagtctcg
    38461 aaatccattg ctcctctaag tctacgggta tcgagcagaa tatgatataa atcataaaga
    38521 gactcaagca taggccacaa ggcttgatgt ttttcatctg ccttgccttg ttccagccat
    38581 gccccaactg acgtgtaggt caaacgagca tgagaatgaa tcactgcccg ataaaagcgg
    38641 gatcgggata tttttccttc ttgggtaatc gataactcag ccaccataca taagcggtca
    38701 acatggggat ttaacgagca aacgccattg gataaggctt ctggtagcat tggcaccact
    38761 ttaccgggaa aataaactga atttcctcgc cttgctgctt cttgatccaa agcagaatcc
    38821 ttcgccacat aatgactgac gtctgcgata gcgacataca attggaagcc gcctttgggc
    38881 tttttgtaac aatacacagc atcatcaaaa tcttttgcat cttccccgtc aatggtcaca
    38941 aaaggaagat tacgcaaatc ggttcgcccc tttaattgtt cctcggaaat ctgttgtgga
    39001 attttaccca cttccgcaag aacatcatct ggccattcga aaggaatgcc gtgagcatgg
    39061 attgctactt gaatttccat gccaggagcc atatgctcac ccaaaatatg gattactctg
    39121 cctatagctt gcgaacgctt gttaggatat gcgatcaact caaccagcac tatttggcca
    39181 tttttggcct cgccaatgaa ttcttgtgga attgaaatat cctgagtcag acgtttgcta
    39241 tcaggaatga caaaattcac gccatgatca gtaaaaaatc ggcctacaac cgtggtattc
    39301 gcatgttcca ggacttcgtg gattttagct tctggccttc ctcttctatc caatccagat
    39361 tgataggcaa gcaccagatc tccatgcatg acagccctca tttctttagc agagagcacc
    39421 atatcctcag aaccatcatc cgggatgaaa aatccaaaac catcaggatg gccttgaacc
    39481 gttccacgca ataaattaat tttttgtaat aaacaaaagc gtccacgcct gtcctgcatg
    39541 atttggccat cacgcagcat agccttcaag cgaaaaccca aggactcctg ctggctctca
    39601 gagctcaaat ccaatttatc aaacagttta ctgcgagaca ttggcctgcc gtattcatcc
    39661 aggatttcca tgatgtattc ccggcttgga attggctcgg cgtatttttc gcgttccctc
    39721 ttataaaagg gatctttgga ttttttactc acttttcacc atttttctct tcaaacctaa
    39781 tgctaactaa aacctcttaa gtcctgttct accatttatc actataacta gcaagaatat
    39841 gctgccaact atcagagaat tctgtataaa acgcctttta ttcatctttt tccaatctta
    39901 caaggcaaga agtcctgctc ataataaaac gaaaatttgc tttagattgc ataattccaa
    39961 tccatagtac tcagtattaa aattttaaat tgcctttagg gctcaagatt gaggagcatg
    40021 gtggcggtct aaactgattc cgttatttgc caaaaactca ggatcactgg gggcagcatc
    40081 ctgatttgta cttttagttg acataattgg ttgcagcata gaatccatag ccatgcccca
    40141 agatattcat cgataaatta tttctccggc aatacaaagc ctcttccacc aactgctgca
    40201 gttaacgctg ctaacgagag gtcttctgct acccagaaag taccttgatc gcttgccggt
    40261 atttttaccc ccttccattg acatacggca atagcatttt cacaaggtat ctgttgttca
    40321 tgccctggcc ttgattcgat gcaatttaaa ataaaggcag gtttgtccac taacaaagct
    40381 gaccatttac cagcctgtaa tcgacttgtc caaccagcac tggccccagg atctgtgact
    40441 ggatgggtaa tccatagatc caaattggtc acattgtgaa taaacaccag cttatttttc
    40501 ttggctttta aagtcactga ttgaccttgt acaggaacag cccgacaacc atcaggaaaa
    40561 gtagattctg caaaaagccc tgttgaaagc aataaaagat aaaaagagag caaaattctc
    40621 atgattcacc ccatagtgtt tatgtaacca aaaattatta aatcttcact aataaaccta
    40681 tcatcaatat cggcatcgat ttaagaaaac actcatataa tatccctact cgtataagac
    40741 ttccattgat aagtcatgca tcgggttgat taatgcattc agaacacctg gctaataaaa
    40801 tatgagctaa ggtataaatg ccctataagg acaatcctac attatacttt caaagttact
    40861 tggtattgca ggagtattat agtcaataga ctgtttggat tgttctccaa agaatgtttg
    40921 agtattgtct gaataattaa caactcgtgt gaagtgcgcc ggactacttt taatagtagc
    40981 taaaagacta attaactgct tgcgtctttc agaaaagcct aaatgcaaag aattggttct
    41041 gctttctgca accggtgcat tgatgagatc ccctgctttt ttacgagtag ggccgggggt
    41101 gcgatcatca caaaacttta taatttttga tgagctgttt tccgcattaa agccataatg
    41161 ccataatctt ttaaaaaatt taattagccc aaaataaaat gtttgcacac tggaactcaa
    41221 atcctgttct acttccgctt ctttggcagc actaatcaat cgttttttta ctcctttatc
    41281 ctcacaatcc ctgactaata gttcacatcc tctttttact aaagaataat gtttattctg
    41341 cccccaatac gttaataaat taatatccgc ctgggggaaa cttgtgtcct tcctggccaa
    41401 ctgttttcca taataaaagg ccatgtcact taaaatttgc ctgtctaatg actgaggttt
    41461 ttttatcatt tcagcttcca gcgcatcgta tatgactgcg gacacatcgc gttgagggaa
    41521 aaatctcaat aattgactga ctggcctgat gtagtctggc attccttgaa aagaattaaa
    41581 ataatcctgt aataaatgca acaatacgct tttcttacct gtataactca gcaaataaga
    41641 acatattatg ggtgacttct tctgtttatc tcttttactc caggtttgct taataaaatg
    41701 actccagtcc agcagaggat gatggtcttt taattccttt tgcaagaaga cttgatcatc
    41761 aaaccaggag cccttaaaaa acaaagattt tccataaagt tccctgattc ctttaggcat
    41821 ccataattta tccaccatcc tgactaaaaa gggttctgtg attgacaatc gggttattgc
    41881 acgattcaac gctttgtgct tgtaaaataa agacgttatc cccgcattgg cgcacctgaa
    41941 aagaagctcg cctaaaacac gatatccaat atcatgttta ttatcgaaag gacgtttttt
    42001 ggataaatgc ctgctggtaa aacaggccag ttcttttaat tctttacttt gccaggcctg
    42061 aggagaatcg agattcaaat cagtttcaga aaaaaggcgt ccatgttgat tagccaaaat
    42121 atgtaaagcc aatttatact gcgcattttg ctcccaataa gaagtcttat ttaactgtgc
    42181 aataaggggc actatatgcc gcctatcaaa aaattggctt actttgccaa tttccggatt
    42241 atttaccttc tgaatggagt tatgagttct atcaccaaga aactccttta acactaatgc
    42301 ggcacatgct tcagaattta ttaaacgatc cagtacagca tcacagattg tctcttgctt
    42361 cttatcttga gaaatttcct tggtaatgaa agtcaaggcg ctacttagaa gatctggctt
    42421 tttatatttt ttaccatggt aagcaaatcg tagtaagact tctgaaaacc agggtttatc
    42481 ataataatta accaaataat cagccaagca aacatcagcg ccttgatgtt ccaaaaatct
    42541 ttgaaaatca gattcataca agccattcag taaaatagaa cgccttgagt ctcggtttaa
    42601 aatataatga aaaagctcaa gcgcattatt tttattcctg atgacgccca ataattcaaa
    42661 tactgtttca tcctcaaaat gatcgagcaa tggatataaa acagatgcag ggatttctcc
    42721 tggagcctct tggatttctt ttaataattt attattcagc catatgcgct tattggcatc
    42781 aaggcaaatg gattcacatt gatataaaac ccctatcgcg ttctcgaata acacattatg
    42841 taacagggac tccaatccaa acgaggaact ttccaaatca cttttggaca aaccagctaa
    42901 aacccaggcg gcagcatttt tgtgaatggc actttcgctt cttaaacaac tctcttccgt
    42961 taaataccct agaaccaaac cctcttgatc atataaaggc tcattgatat ccatagagcc
    43021 cgttcgagct aataatacga tttcctcatt aacaaaagtc attgcagcat tagactcacc
    43081 aagaaatcca atcttcttcc cggcagagtt atataaaaaa atttcttttt ttctgtctaa
    43141 ttctttgggc aacttatcaa ttagaggaaa actttgtttt aaccagtgaa ataacccgtt
    43201 ccgagcttcg gtttcattat tccccttaat aaaaaaccgt gaataaatat gatttaccac
    43261 tttaaggaat aaatccgaac gtaaattggg ataactcaag gcgtaataat gatataacac
    43321 ctctaatccc ttattatgta gctcatctcc tcccttgttc gcctccagta aacactccat
    43381 ttggtgaagc aaagcacttt tactaatctc aactctaaac tgcaataagc tgtatttttt
    43441 atccagccaa aatgactgag cttccggatg aatacacaga ttacgctcaa tctgagccat
    43501 atgctcattg atttcatcaa aatatggagc atattcttta aaacaaaggt cttgcttata
    43561 gtccttctga ttcagagtta aggtatacaa gctgtatctt tcaactaatt gttttaaaac
    43621 cttaggcgta caaaccccga caaattgtgg catggataac aataaagcga ttggataact
    43681 ggataacggc tcctttcctg agttatatga tttagttatt aaatcggatg ccagctgata
    43741 tagctgaggg gtatcaaaga gataaagatc cagttgatta atttttgttc ttatttcccc
    43801 caaaaagtcc tggctggaat cagaaaaagc cgaacataac ctggtaagca agtttttaca
    43861 gttctgcttc tcttccccag tcgttttgtt atattccgct atacaatact caataatcaa
    43921 aagcacagtt tcctgactta agccctgaaa aaactcttgt tgtttgtcta atttggtgtt
    43981 ttttaataaa tcatgcagca actgcatgac taaatggctt tcaggagaaa ataaatctct
    44041 ttttaattca tactgataaa aatcaaacac agcccgcatg gcatgcaaat ctatggaaaa
    44101 aggatactga gcaaggcaga ctctagcctc cttcagtttc tcaatcgctt tctcagaaga
    44161 aaccaaagac aacaacaatc gaattgagag agcattgata gattcatcgg ttaatgaatc
    44221 aaggaaacgg gttaaccatc ctgccaaatc aggttgacgt aaaaattgta ccaaatggtt
    44281 tctattgact aaatccaaat ctttattgtt tttgactaaa aaggtttcaa ttaaaacatt
    44341 taaatcggta tctttagcca gatgagaaaa agctgacaaa ctgcgctcat cggcttgctc
    44401 caagatctga attaaatcct tggctcctct ttctttcaac gcgataagga caggttttgc
    44461 atcactataa taattcaatt gcctcgtgca tctctctgct aattgattat caacactggt
    44521 ttcggtctct tttattgaat ctaacattgt cagcaactgc tctgataact ctcggtgaag
    44581 cagattgggg gcataagtca aaaagattgt gagtacagat agcgaagccg gtaaaccaaa
    44641 tgggactgag tgattaagcg agaattgatt tttctgagcc gggatttgtt ggcaaaagag
    44701 ttctgttaaa tcgtgagcat cttctttaag gtgacttaac aacaaatgtt tcagatcggg
    44761 aatagggagc tgttgaattt gtccttttat tatacttatt aactgcttca ttacgacact
    44821 tccctaacat cttaaaataa tcctttcccc aatagggatt taaaaggata atatttttac
    44881 tataccctta aactattgat ttgaaaactt taggatagca aatgacgatc tggattgatg
    44941 ccgatgcttg cccgaaaatg ataaaagata tcctctttcg cgcggccata agaaccaata
    45001 ccaatcttat cctggtagct aattcctacc ttacttatcc aaattcaccg tttattcgct
    45061 cagttcttgt agaaaaaggc tatgaccgag cagatcatta tattacgagt catatgaaag
    45121 ctaaagattt ggtaatcaca gcagatattc ctttggcagc agaagtaata gttaagcaag
    45181 ggttagcaat gagtccaaga ggagaactgt ttacagccaa taatatcaaa cagcgtctaa
    45241 ctcttagaga catcaatgaa caattgcgtt cagctggaga gcgcacagga ggcccctctg
    45301 cattaagcgc gaaagaaaaa accaattttg ccaatgctct ggatcgttgg cttgcaaaaa
    45361 gcaaataaaa gcatcaatta aaaaacatat tgactttaat ttgacaaaat cgattgctcc
    45421 tcctaaattt taagtacatc ctaaatggac ttaaaaacat gaaacgattc atgttcattc
    45481 ttttgatgtt tttaaatttt tacacctttg gaaaaccact tatcattggt gttccggaat
    45541 ttgcgccccc atttgtatca agatctggag gaggaggaga gacatatttt ggatttaaca
    45601 tcgatttgat gaattccatt tgcaagatca ttgaaatgga atgccaatac aagggcatga
    45661 atattcaaca gctatatacc aatcttaacc aagggaagct tgatattatg ctggctccta
    45721 ctccaatcgt atccactgtt ggcactaatt atatattcag tcttccctat ttaccgagca
    45781 atgcacaggt gataaccctg aaagctaaca atgacatcaa tagcttagca gatctgaaag
    45841 gtaaaaaggt tggcacttta aaatacacgt tgtatggtag cctccttgca gaacattttc
    45901 aggattattt tgacctggta caattttcca aactgtctga catggccctc gcattatcaa
    45961 accaaaaaat tgaagccatt gttcttaaca caaatgcagc gaaatatgcg atgagcaatt
    46021 ctgcaactga gttaaaattt gtgggcgata aaatggctat aggtgatggt tatggaatac
    46081 tggccctcaa aaataatgct cctctcatta tgaaaatcaa taaggcttta ttacaaatag
    46141 aaaaagacgg gacctatttg gaaatctata atacctattt cggtgattag gatattatcc
    46201 tgagcaattc accaggcatt tcaaaaaccc gaaactagat ccaactccag aattcaggtt
    46261 aaactaatac caattgaacc tgcctctcat aatgcattga gcctcaatgc atgagatata
    46321 gttcaaaaca gtcgagttca agtgatggaa ataggtatat tttcataggc taaactcatc
    46381 ttaaagtaca atgcaaatta atctgcagga tatgacatgc aagaaagttt tgatccatca
    46441 actatcacta cccttcataa aacgagcatc atacgctgga aagccaatgg tatagagtat
    46501 caacataaac aattccttca actggtagaa gaaaatcatg cttttaattt ccaattatgg
    46561 catgcagaag acaaggcacg ccgagatgat atgggctttg aatttgttta ccaagccaag
    46621 cgggaaatag atgcctacaa tcaacaacgg aataaccgca tggaagcaat ggatgaatgg
    46681 ctatataaag cattagaacc tgaaaaccat aatcattgcc ccgtcaattc cgagtcacct
    46741 ggaatgatta ttgacagact atctatttta tcactgaaat cctatcatat ggacttgcaa
    46801 acaaaacgcc aggatgtgag cgaagaacat agaattgcct gcacacaaaa actggaaatc
    46861 atacagcaac aattaaatca attggctttg tgtcttgatg aattacttaa tgaggtacga
    46921 gctaaaacac gtacgttcag agtttaccat caatttaaga tgtataacga tcctaagcta
    46981 aacccagaat tgtactgttc tgactaggta ttaattgatt taatgctgac tcgcttttgg
    47041 aatgaggaat tgaaggagag tcgggttgat ttcttttggt tttggtttca aatataccga
    47101 ataaagacag agaatccgta tgccctgggg aatcagtccg agttttttca gattggattg
    47161 attccctttg ttcttcggtt ttctgcataa cttccttcgt tatctcaggg ctaaccgacg
    47221 cttctttgct gtcctccgtc agcttggcct gctgcgccaa gggagttcgc cccaatcctt
    47281 tggctagagc tcttaaagcc agagtaaccc caaacaataa agcactgaag acagataaga
    47341 cgtacataac aggcgtatca cgataatggc catcctcgcc cagttcctgt aacgaattcc
    47401 cagcaaagtc aataaatttt tgccccttac caaatccgga aaataaggaa cgcaaaactt
    47461 caaaccattc ttgaaagaaa aattgcggtg aaggatctaa agacaatcca tcatttagta
    47521 atttatgaaa ttcttcagga gccaacagct cagtcttatt aaattcatca ttaagactat
    47581 ttttccattc gatcaatcgc tcatatggac gttccttttg agtttcctgc ttcttctgat
    47641 gccaataatt actcaccaaa gaatgaatag tgaaactgag cactaatacc agacctaaaa
    47701 taatacaagc ggtaagcaag gcaggaggaa aagaaacacc tgccattact aaaatagaac
    47761 ccactaaaaa caaaacactt gcaagcgcac cataagcata taatccattt ttcataccca
    47821 acaaagcaga tgataaatat gtgcggttcg attgttggga taataactta cgactgtcgt
    47881 caaaccgggt tatcaaatta tggactaacc tttttagtcg tttgagctct tcctgatcaa
    47941 ctttgtccag atccattttg tcttgcaata agagtaattt ggcgtaattg gtttccagct
    48001 ccttggtaat taaagccaat tgacatttgg tttgcgtcac taataatctc aactggaaat
    48061 catattcctc ataaatacgg gtaataacac aagctactgt ataaaaagca caaataagcg
    48121 ccattgccat aagcaactgc ggtgacaata cagccagact caatacacca acgtagagat
    48181 ataaaccatc aagaagtccg cctgcagcaa cagcccagaa tgcgaaatta cgagtttgca
    48241 tggtctggta tctgattcgc ttaaaataag aaaagctttc ctcatgagat aaagaaatta
    48301 atttttttat ctccatgaga agcgctatat tctcactcat cattatttta cgatcttcca
    48361 gcatgctacg tatccaaaac ctgttggctg cagcaaatac ccccaaaatc actcccattg
    48421 gaagaataag aaattttgca tccactcctc caattaaact gaatgcaaca acagcgcttc
    48481 tccagccttt gtaagcattt ttcaagccct tcatgacatc acgaaaatat ggccaggcat
    48541 tagcaataaa ttttttatag taatcttctt tttcattctc aaattgacag gccaaaacag
    48601 aaaaggtaac taaaaatact gactcaactg caatggtaat aagaccggct ggagaattta
    48661 agaattcatg catcgcatcg ttatcattag gagatgcaaa cagatcaaaa aaatatttga
    48721 acatgctgta cgaggaactt agggaatcca taacagcgta ggcataatag atactgcgac
    48781 ttttatcgag tttttgagcg agaattctac tttgcgcgtc gatacgacca gcaattgcta
    48841 aaatgaagac caaatcagta ggcttggaag cctgattttc atcactcgca tgaggcatgt
    48901 ataggtatgg ggctatcagc taagattggg ccctattgta tgcataatta gacttgatgt
    48961 caattaaact ttcatcctga gttaaaaaaa tacaacaatg acctcaaacc cttcataata
    49021 acacatccta ttagcaaaat tattaaaaaa aataaaaaaa attgcattaa attctctctc
    49081 agactgtgac ttatgcctta aacctgctac gatattgcac caaaataaaa cccaatatga
    49141 ggattaaatg agatcaaaat ttgttctctt tatgttaatg tcactactga caggatctct
    49201 tatggcaaac agtgaagctc aaatctatgg ctacgtagag aaagccacat taatcgatca
    49261 aaacctcact ttatctgcaa agcttgatac tggcgctaaa tcagcatcat tacatgccgt
    49321 gaatatcaca gaaattgaaa aaaaaggcat tccttattta cgttttaccg ttccaaccaa
    49381 aaccggtgat tactcatttg aaggagagta tgtcggcaaa gtaaagatta aagtaaggtc
    49441 aagtgagacc aatccagggt tattaagaac aactcccatt aagcgaccgg tagtcttact
    49501 taatatcaag cttggtgata aagtcagaac aattaaagtg aacttaacca acaggaaacg
    49561 attcctttac cctttattgt tgggaagaga tgccattatt gattttaatg gggccgttga
    49621 ccctgccctt actttcacaa ctaaatccaa gagcccagtg aacaatgaaa aataacaaac
    49681 gtcatgttta tggtttgatc ctcactctat tgcttttagg atcaggtatt tttttatata
    49741 gacatatcgt tctggatgtc cctctgaccg atacggaaac gatcaatagt tggatggttg
    49801 aatccaatct acgttttacc gctgatagaa atacgccgat aaaagcaagc tttaatatcc
    49861 cttacctgcc ccccaatttt gccattctcg atgaatattt tgtgtccaga aattatggtg
    49921 tcaccaccaa tttaaacgga tccaatagag aaactgtatg gtctataagg cgtggtaatg
    49981 gtcctcaatc actttattat agagcaatct tccgccaaac ggacagtgat gagtcttctt
    50041 tgcctaaacc ttcggtaact aaatcacaac ctctgaatga cagtcaaaaa tcggccgttg
    50101 aaacgattac caatcaagtg agatcaacat cagcagacat tcaaacattt gcccaaagca
    50161 ctattaaaga gttaaacaaa agagatggta atgcgaagct tttagttggg aacgaattca
    50221 atgatgacaa tattatcaat gccaccattt tgattctcaa tcaatcaaag attccggcca
    50281 ttacagtcca ggggatttat ctaaaccaac aaaaaaaggc cgatttaaaa tccctgctcg
    50341 ctgtgtttaa tggaaaaaac tggatttaca ttaaccccaa aactggcagt gccggcttgc
    50401 caaaagagtt tctgatttgg caatatggga atggccctct atttaatgtt gttgggggta
    50461 atagagctca attttcatta actgtatcgc caacacccat taatgcctta agtgtggcca
    50521 aatcaagagg gctagaagac tcgcaactct tgcgcttctc cttactccag ctacctgtca
    50581 atgtacaagg gatctataaa atcttgctaa ctgttcctat tggagcattc atcattttaa
    50641 tattgcgtaa ttttataggc attaaaactt ttggtacgtt tatgcccgtc ttgatcgccc
    50701 tggcctttag agagactcat gtagcatggg gcattacttt atttgtcatt attatttctt
    50761 tcggactatt agcccgattt tatctggatc aattaagact actgttagtc ccaagacttg
    50821 cagccattct cactgtagtg attctattaa tgatctttat cagtgttcta tgccaaaatc
    50881 ttagtcttga taccggtatg tctgtcgcat tattccctat ggtcatctta accatgacta
    50941 tcgaacgcat gtgcatcaca tgggatgaaa gaggggcttc cgaagcaata aaatcaggaa
    51001 ttggcagtct tgctgcagca gttatttcat atggggcaat gagttatgag cccttacaat
    51061 atttgatctt tgcctttccc gaattacttt tggtgctttt gtccttgatt ttatggttcg
    51121 gtcaatatcg tggctatcgt ttagtcgaat taaaacgctt taaatcactt gcgagtgcga
    51181 tgaaatgatt aacctgttta ggcgcctgaa aaatcatggg gttttaagca ttaaccagcg
    51241 gaatacggat tttgtattac gatacaatcc aagaaagtta tttcctttgg ttgatgacaa
    51301 gctcaaaacc aaaaaactgg ctttaaaagc tggaattgca gttcctgcgt tatatgacat
    51361 cattgaaact gagcaacaaa tcaaatcaat agaaacccga tttgccagct ataatgattt
    51421 cgtggtaaaa ccagccagag gttctggtgg tgatggtata ctggtattta aggacaaagt
    51481 ttacggtcga tatcgacaaa ttaatggcaa gcttacaaca gtgcaagaac tgagttacca
    51541 cttatcttgc ttgctatcag gggcttatag ccttggtggc tcctcagact atgccatcat
    51601 tgaaaaacga gtagtcgtcg accccgtgtt tgcagaggtc agttatgaag gcatacccga
    51661 tatacgtatt attactttac tgggctatcc ggcaatggcc atggtaagac ttcccacccg
    51721 tttatccggt ggaaaagcca acttgcatca aggagcaatt ggtgtaggcg ttgatcttgc
    51781 gacaggaaaa actcttggtg gtgtgtttca aaatgatacc atagactacc atccggacac
    51841 gttaaatccc attgtaggca tcgaagtacc ctattggaat caaattcttg aaatagcctc
    51901 cagttgctat gagctaactg gtttgggtta tcttggagtt gatattgtac ttgataaaga
    51961 acatggtcca ttaatgctgg aattgaatgc cagaccagga ttaaatatac aaatagccaa
    52021 ccgcgagggt ggccttaagc gttatagaac gattgaagca cgttttaaag agagcggaca
    52081 agagtcaact ttagataaag taagtttttg ccgcaaagaa tttgcacgct aaagtgcggg
    52141 ggaagcagag tgagctggtt ctttatttaa agacagatta ttctgctcct ttaaaaaacg
    52201 gcgacgagca tataaccaat cgatcgtttc atccaactca acacgttctt ttttagactg
    52261 aatttttgac agctcccctt gcatcctgct ccaatataat cctgttacat cgtatccgtt
    52321 ttcagatcgc tgtagcgcag ggcgcaaaca tttttcatca attttttttg tcatctcttc
    52381 ccgctgtttt aataattgct ccaaaatcaa ttgctcagca tggatgtttt cttgaaatcg
    52441 cttcaaccca actcgacgaa gttcctgttc aaactctcgc tttacagatt caaaggtagt
    52501 ctccgcaggc attcgaccca aaaccatctt tgataaatca agagttggct tgtttacagg
    52561 gaggcgcagc cataatcgtc caaacatgaa ctctccattc aaatcatcaa tatattgcat
    52621 tattgcaaaa caaatacatt tagcaaataa atcgactatc aattttaaaa catgcgtttt
    52681 aatttgactc acaatgaaat ataataagac aaaagatggt tttaattatg caattaacag
    52741 aatctacctc ctttctgcat atgaaaaaga gagaattaga ttttgtttaa gatccatctt
    52801 tagtgcaaat tattaacaga ataatgctga cctgctggca taccaaattt gtaagggaga
    52861 tttctcatgc ggtctaaatt tttttcattt ttcacacgtg catcaagacc gatgaaagct
    52921 atccccccta aaatttggtt tgaaacacaa ttaaaaggca gtggcctcga taaaaaattt
    52981 cagatagatg agttgataga aacgcaatct tccgtcagag tatttgctaa taagaaatat
    53041 cttccagaca ctgaaacgat taatgaggca ttaactaaag taacagccgt caatgtgtca
    53101 ggagataaga gcgggtattt tcaaaatgga ttaccgttcc caaatgaagc aggctatttt
    53161 gagaaaatcc ctgtagggca tcctgaatta ttgtctccaa tagaaagatt aacaggcagt
    53221 aaaaaaattg tttcttcgca tagcctggtt accgcctccg gtggttatcc actaaccaat
    53281 ccattattac cctatcgaaa accaataaga gtgagtattt tttctttagc aggcccctct
    53341 tttgaaaata attacctcca ttatcgttta tttctattag actccgtaca aaaaataatt
    53401 gattcaccac tctttagcca tttgcatgat ggtttaccta ttcaatttga tgaggctaaa
    53461 aaggagttag gtgagtatga tactaacaag ctgatggctc gcattcgttt ggggtttcct
    53521 tatctggcaa ggttttcatc aggtggtttt tatcccagtt ttagcaagtc caacgctatt
    53581 atcttcctat ctgaagccta ttttcgctat caactggaag acgtgtcact actgctggca
    53641 tcagtaaatc aaacaggaaa agaaactgga aaagcggcct tgcttaaagc cactgcggtt
    53701 ggaatgggtt tttttgcaaa aattgattgc ggttacgata tacaacatat catttttccc
    53761 tattatttac gtgcttataa aaaactgtta agtgaacata aatttccatg gattgcaaaa
    53821 attgaatttc ccatttttaa tgaaattcag caagaacagt ttgattcaat ttttgaagac
    53881 tatgatggcc ctaccaaagt gtatcgaagc acacgcgatg ttctggagtt tcgtgaagag
    53941 gaaattgaaa aatatctgcc tgctgctatt aatccttcag atgcgtttgc tctaactgga
    54001 aatgaatggg gatatggtag cgttgaatca atgattggga ataacagttc tatacgcttt
    54061 gaccaggtac atcatatgaa tccgcttatt ttagatccat ctcaccatgt tgaagcacaa
    54121 ataaataagg atcatggcgt tgaattgaca gtcaatccaa ggccacaatc ctcatcgagc
    54181 attaaactct aggggataat gaagattcct ttacataata agtaacagcg tctgcaagat
    54241 attgatctcc gtcaaatcgc ctcagcaaaa ttttaagagg agtatctcca tatacttctt
    54301 tgcaaagctt ggcaaactct tcctcacaat catttatcaa taaccattcc aaagtgcttt
    54361 tttccgaact gaaatcacca agtttcattg tctctgggac cttggggtta aaatccttca
    54421 tagcattggg aaccactccc ccatcaaggt atttatccct gtattttggg caagtagacg
    54481 cgatgatttg tccaaatgag acactatcga caaccagttc ttctttttcc ccgtcatgct
    54541 caccacatcc tattcctact tcagacttta gagtaagaag tgaagttgaa ctgccataaa
    54601 atttatgaac ccatgcttgc ttggttaatt cgggataggt ttccaggaat tgtcgtaaat
    54661 cttctgtaga agcacaggca ccccatccaa atggattcag attaaatttc tggtatcttt
    54721 ctacatcggt aatacgaacc ttttccagct tctcgctgtc aacgcgttgt ggcaaaccac
    54781 ccaatttgtg taaatgatct aaactacgcc catcagctcg aaccaatatt tttttagtct
    54841 gtaattcctt gattttaaat ccaaccagag gatcgcttaa attagaaata ctggattcaa
    54901 atttaagtaa cgcattgtga aatttggaaa aaaattttaa tctgttcatt gcttaacctc
    54961 tgaggaatga taggcaacct aaaaaacttc agaaataaga ataagtgatt taaaaacctc
    55021 ggagagtttt
//
LOCUS       DACTJE010000003        47070 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.003, whole genome shotgun sequence.
ACCESSION   DACTJE010000003 DACTJE010000000
VERSION     DACTJE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 47070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47070
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.003"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(275..1937)
                     /locus_tag="I9760_000117"
                     /pseudo
     CDS             complement(275..1937)
                     /locus_tag="I9760_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947875.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family peptidase"
     gene            2191..3045
                     /locus_tag="I9760_000118"
     CDS             2191..3045
                     /locus_tag="I9760_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAT5041920.1"
                     /translation="MNFNIIGAGRLGKNLGIAMSVHQIASLNSICNSNLASAKIACNE
                     IGFGNAVGKISDLSEADITWITCRDDSILDVVSILDNIEILKKGSLVVHCSGALNSSV
                     LLPLKKQGCYIASAHPLKAFRTNSLDSNAFKQVHCVIEGDYEACRWLRFAFEKLEASI
                     IAIQPEMKVIYHTAATIASNYLVTLASYSEELLLKAGIQQAQSRKMICDLMYSNILNL
                     SQVDSAKSALTGPLTRGDMQTISLHLEAIDNSEIKRLYKSMALATLPLLDLSLDRKET
                     LRKTLEKE"
     gene            complement(3152..5407)
                     /gene="parC"
                     /locus_tag="I9760_000119"
     CDS             complement(3152..5407)
                     /gene="parC"
                     /locus_tag="I9760_000119"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAT5041921.1"
                     /translation="MKKIPNMNEVTEKKPLTEFTEKAYLDYSMYVILDRALPSIADGL
                     KPVQRRIVYAMSELGLKATAKYKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYR
                     YPFVDGQGNWGSPDDPKSFAAMRYTEARLSHYADVLLGELLQGTVDWVDNFDGTLQEP
                     ALLPARLPNILLNGATGIAVGMASDILPHNLTEVANACIHLLDNPQADIKTICEYIKG
                     PDFPTEAEIISPSETIRAIYESGNGSIKMRAIYTQEKQNIVITALPYQVSGARVMEQI
                     ALQMQQKKLPMVEDLRDESDHEHPTRLVIIPRSNRVDIESLMSHLFATTDLERSYRVN
                     FNMIGLDGKPRVKGLLAILTEWLEFRLATVKRRLQFRLEKVLERIHILEGLLIAYLNI
                     DEVIAIIREHDQPKQGLISRFNLTERQAEAILEMKLRHLAKLEEIKLRGELDELSDER
                     DTLQKTLASHSLLKELVKEEITKDRDEFGDPRLSPIITREDSQALKEEDILPNEPITV
                     VLSKNGWVRAAKGHDVDGKELSYKAGDEFKAQTLARTNQQVLFFDGEGKVYSLPGHVL
                     PSARGQGEPLTGKLNPAEGVLFEAIVGGEPEQKVLLATDSGYGFIAKIEDLYVKNRNG
                     KACIKLSENSHILPPRLIPNREQLFVACATNVGRLLIFSLEELPELSRGKGNKLISIP
                     VAKAVSREEYIVDMQVLTTNDSLTVYAGKRHFTLKGEDLTHYKGERGRRGNRLPRGLQ
                     NVSQLHVTQSE"
     gene            complement(6035..6457)
                     /locus_tag="I9760_000120"
     CDS             complement(6035..6457)
                     /locus_tag="I9760_000120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAT5041922.1"
                     /translation="MSITTHLDIVSAEHEIFSGVVEMVVATGELGEIGITPGHAPLLT
                     VLRPGEVRITLQGGTQDIYYVQGGMLEVQPHCVTILADVAERAEHLDEAAALAAKAKA
                     EAAIASKGGDIDYSVAAAELARAVAQIRAIQKTRKKMK"
     gene            complement(6469..7845)
                     /gene="atpD"
                     /locus_tag="I9760_000121"
     CDS             complement(6469..7845)
                     /gene="atpD"
                     /locus_tag="I9760_000121"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAT5041923.1"
                     /translation="MSLGTVVEVIGAVVDVEFPRDSVPKVNDALKLVDSDLVFEVQQQ
                     LGDGVVRTIAMGTTDGLKRGLKAENTGHPIQVPVGKKTLGRIMDVLGRPVDDAGPIDA
                     EETWAIHRKAPSYEEQAGSQELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LIRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRV
                     ALTGLTMAEKFRDEGRDVLLFIDNIYRYTLAGVEVSALLGRMPSAVGYQPTLAEEMGM
                     LQERITSTKTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRQIAELGIYPAVD
                     PLDSTSRQLDPLIVGQEHYDTARRVQQTLQRYKELKDIIAILGMDELSEEDKRVVTRA
                     RKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIKGFQGILAGEYDDLPEQAFYMVGSIEE
                     AVAKAKTL"
     gene            complement(7858..8724)
                     /gene="atpG"
                     /locus_tag="I9760_000122"
     CDS             complement(7858..8724)
                     /gene="atpG"
                     /locus_tag="I9760_000122"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAT5041924.1"
                     /translation="MAGAKEIRSKISSINKTRKITRAMEMVAASKMRKTQERMRASKP
                     YANKIYEVIKHIARAASEYRHPFMSEREIKRIGIIVVTTDRGLCGGLNSNLFRETIRT
                     IRNWQEHGKEVDIAVIGRKGQAFFRRVGGNILGSIDHLGDTPSINDFIGVVKIMLDAY
                     YNGTIDSLHIVYNEFINTMTQKPFVKQLLPLPKSEEDKKTLGHHWDYIYEPEAKELLD
                     EILERYIELQVYQAVVENIACEQAAKMIAMKSATDNAGDLIKEFQLAYNKARQAAITQ
                     ELAEIVGGAAAL"
     gene            complement(8814..10367)
                     /locus_tag="I9760_000123"
     CDS             complement(8814..10367)
                     /locus_tag="I9760_000123"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAT5041925.1"
                     /translation="MSEQVALNPSEISELIRKKIDQFSVVSEARNEGTIVSLKDGIVR
                     LHGLADVMAGEMIEFPGGVYGLALNLERDSVGAVILGDSSTLAEGQKGKCTGRILEVP
                     VGKGLLGRVVDALGNPIDGKGPIESSGMSPIEKVAPGVITRKSVDQPVQTGLKAIDAM
                     IPVGRGQRELIIGDRQTGKTAIAIDAIINQKGTGVKCVYVAIGQKASSVASIVRKLEE
                     HGALEHTIVVVAGASDSAALQYIAPYSGCTMGEYFMERGEDALIVYDDLTKQAWAYRQ
                     ISLLLRRPPGREAYPGDIFYLHSRLLERAARINADEVEKLTNGEVKGKTGSLTALPII
                     ETQAGDVSAFVPTNVISITDGQIFLDVDLFNSGVRPAINSGLSVSRVGGAAQTKIMKK
                     LGGGTRLALAQFRELEAFSQFASDLDDATRKQLERGQRITELMKQKQYSPLTVAEMGV
                     SLFVVEKGYLDDVPVNEISSFEASLHDYMRSTHAALLHAINEAGAYDNEIEAKLKKAV
                     EEFKNTGSW"
     gene            complement(10385..10927)
                     /locus_tag="I9760_000124"
     CDS             complement(10385..10927)
                     /locus_tag="I9760_000124"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAT5041926.1"
                     /translation="MSDSTTIARPYAKAIFEHALAEKKLSEWSEYLTLLAQVVLTPQA
                     TQFIANPASTDEQQIELLIEVCGSKFKKNDALNNLIKLLTTNKRLMLLPEIEALYEVY
                     RAEQEKILEVDVVSYSELTPAQQQRLSESLSQRLSRKVSLKISIDPSLLGGALIRAGD
                     LVIDGSVRGKLNMLGTSLAA"
     gene            complement(10929..11399)
                     /locus_tag="I9760_000125"
     CDS             complement(10929..11399)
                     /locus_tag="I9760_000125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAT5041927.1"
                     /translation="MDINLTLIVQMLVFAAFVLFTMKLVWPPLAKALEERQDKIADGL
                     AAAERGRKELELAQHRVKDELKQAKAHSADIIDKANKRASEIIEAAKEAAKREAQIQA
                     KLAQEQIAQQVNHAKEELRKQVAKLAITGAEKILMREVDAKANSELLDNLIEEI"
     gene            complement(11451..11726)
                     /gene="atpE"
                     /locus_tag="I9760_000126"
     CDS             complement(11451..11726)
                     /gene="atpE"
                     /locus_tag="I9760_000126"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAT5041928.1"
                     /translation="MQAAELIAQVQSMTVIAVALLIGLGALGTAIGFGLLGGKFLEGS
                     ARQPEMVPMLQVKMFIVAGLLDAVTMIGVGIALFFTFANPFLSSLGS"
     gene            complement(11767..12594)
                     /gene="atpB"
                     /locus_tag="I9760_000127"
     CDS             complement(11767..12594)
                     /gene="atpB"
                     /locus_tag="I9760_000127"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAT5041929.1"
                     /translation="MVSSTNYIKHHLTYLTYNVNDMTFGSSGGFWTLNLDTIFFSIAL
                     GLIALAIMYLAARKVTTGVPGKLQNFAELMLEFADNQVKDCFHGKNNLIGPLALTIFM
                     WVFLMNFMDIVPVDILPVLAQLVGIHYLKVVPTNDLNLTFGLSLSVFFLIVFYSLKIK
                     GVKGFVKELTLQPFNHPLFIPFNLLLELVGLIAKPISLALRLFGNLYAGELIFILIAL
                     LTLNATTSSLISTMTLGTAQFILALAWSIFHILVITLQAFIFMVLTIVYLSLAHEDH"
     gene            complement(12596..12991)
                     /locus_tag="I9760_000128"
     CDS             complement(12596..12991)
                     /locus_tag="I9760_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAT5041930.1"
                     /translation="MNKQLNKRGITRLWLAQLGVTIILAGLCAILFGVNAASSALLGG
                     LVCIIPNAYFAIKLFKHQGARAARQIVNSFYKGEALKIILSMFLFALVFLLCRITPAA
                     FFASYILVLMTHWFAPWIIVNKQNRPKSD"
     gene            13145..13390
                     /locus_tag="I9760_000129"
     CDS             13145..13390
                     /locus_tag="I9760_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041931.1"
                     /translation="MSEKIQHTAARLQEKMSNRYSDGLSHKHNKNHLSYRQHCRSHPC
                     NQTNNNDEENIVNGSTGLLREFNLRKIRKRLDKGRFH"
     gene            complement(13460..14047)
                     /locus_tag="I9760_000130"
     CDS             complement(13460..14047)
                     /locus_tag="I9760_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HAT5041932.1"
                     /translation="MFKHGCFILIVFFLASCLSGCLGVLWTGATLAYDRHNVYKKLDD
                     YNLIKALNDVLAVNRTFKNSETVLDIAVFNRDILIAGHVPTQELYDELQLRLSKVKGY
                     RRLFNRVIINKMPSNSIQDSWITTKIRSQIFADSSIDPNAFKVVTSDRVVYLMGDVQT
                     EQAEKVIKIARYTGGVQKVIKLMRYYTYQTSTNMA"
     gene            complement(14049..14615)
                     /locus_tag="I9760_000131"
     CDS             complement(14049..14615)
                     /locus_tag="I9760_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HAT5041933.1"
                     /translation="MNLKFKSIIVMMIGALLTGCVAVVVAGAAAGMVYDRRSVTTMEA
                     DARLFHVIHKTIIKDPRFRHSRILVSSFNRVVLLVGQTPAASLRVLAERIAQNAPGVK
                     RVYDEITVDNPIPLTQRTKDSWITSQVRSNMLTKKGLESGSIRIVTENGVVYLMGIVS
                     DEQALLAVDVARRVAGVRKVVKIFQYIR"
     gene            complement(14625..15224)
                     /locus_tag="I9760_000132"
     CDS             complement(14625..15224)
                     /locus_tag="I9760_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAT5041934.1"
                     /translation="MIQLEERVRHLFGVTIEAKISAADVLSGSIAKAGKLLVNCLLND
                     GKIFICASGGSSANCMHFSSSMLNHFEVERPSLPVINLCADASCLSSFADEHHFGQVF
                     ARQIQALGQEQDVLLLLTTSGNSDSMLQALHAASERGMDTIALSGRDGGVLANHMGPE
                     DIEIRVASDSTALIRELHLFILHCFCDLIEQSLFGQVLG"
     gene            complement(15251..15607)
                     /locus_tag="I9760_000133"
     CDS             complement(15251..15607)
                     /locus_tag="I9760_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAT5041935.1"
                     /translation="MTQEKGKFAEQLALNYLKENGLALVMQNYHCRLGEIDLIMREGS
                     YLVFVEVRSRSNMNFGGGLASITYEKKQKIIKATSHYMIKYRIQDKFPIRFDVISIDG
                     KSNKITWLKNAFDAGC"
     gene            complement(15629..17440)
                     /locus_tag="I9760_000134"
     CDS             complement(15629..17440)
                     /locus_tag="I9760_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT5041936.1"
                     /translation="MLIKQLKLRMFLVLVSLFLLCQCTKTVNSTEPVVTVNKKLKSPY
                     SLPTAAYLAMAKNQQGHEKQSALISAAGRLITDGQWKQGTAILAQTGELTVEQANEKN
                     LLLAKVDIMRDRPRDALAKLARIKEPERLSLYNQIQFHEQLAKSFRSIGSYSESVSER
                     IKLESLLPDEENQVNNRRALWLTLTSLPQEELNTMALEAVNKSEMQGWLQLAVISRKY
                     RNNSKSLLAALDQWQMHFSNHPANQILPNPLDSISGKLLAPPKQVALLLPLSGPLSGP
                     GNAIREGFMAAYKANRGDESTKIKVYDTSKDDITNTYRQAISDGAEYVVGPLTKTQVA
                     TIASMEHPVPTLLLNDTDTSTQSNSYSLGLSPVNEAIQVAIKARSKGYKKALIIAPNN
                     AWGNEVTKAFINQWNEDGGHIVDSLRYEAKQDLNKSMKDFLQITNSQEREKKLKQVLG
                     YNFQSTTSRRQDFDMIFLLAYPSKARQIMPLLKYYYAGDAPVYATSSVYGGSANALKD
                     KDLDGIIFCDMPWVFSHQMGTKNWPEQFNSYNRLYALGVDSYTLATQLNQLILFPADG
                     SNDSTGILYLKPTQQVARVLEWGQFKKGLAHSLGDTV"
     gene            17480..18331
                     /gene="rsmI"
                     /locus_tag="I9760_000135"
     CDS             17480..18331
                     /gene="rsmI"
                     /locus_tag="I9760_000135"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAT5041937.1"
                     /translation="MTNTLATGLGTLYIVATPIGNREDISFRALNTLKSVDLILAEDT
                     RHSMQLLTSLGIKNNLASLHTHNEANKSNEIIELLLHGKSIALISDAGTPLISDPGFP
                     LVKQARQHHIPVVPVPGACALIAALSAAGVPCDSFAFLGFLPAKQSARKHALESSRSV
                     PYTIVFYESTHRIIDCLNDIAEIYGQDYELVLAKEITKTFERFVSGKIKEIKDWLLSE
                     PGHIKGEFVLIFPPRSTNKDPNSHEELLKILLEELPLKQAVAIACRLTNANKNQLYEE
                     ALKLKES"
     gene            18461..19528
                     /locus_tag="I9760_000136"
     CDS             18461..19528
                     /locus_tag="I9760_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HAT5041938.1"
                     /translation="MDDLKKYSFLLVYLLIPLPFVSLYLGGWYSFLPFFIVFAFIPLI
                     DAFLHDSVNPTKEQEQELLKDKFFKYITCAYVPIQITIIAYGVYIVSTQNLVWNEWLG
                     FTMSLGLISGGVGINFAHEMMHKNSKLQQLMSKILLVTVCYGHFFIEHVKGHHVKVAT
                     PDDPATARLGENLYQFLPKTLLGSFQSALILEEKRLSHKNYSIWSIHNNFWWILTVPL
                     MICIVCFIFGGLIALSFFLLQSFIAILTLEIVNYIEHYGLERKMLSNGQYEKVNPNHS
                     WNANHWLSNQLLIHLQRHSDHHAHGARPYQILRHMENSPQLPSGYLGMIIIALIPPLW
                     HYIMDKRVLAYRIQLRQLEQL"
     gene            complement(19576..21741)
                     /locus_tag="I9760_000137"
     CDS             complement(19576..21741)
                     /locus_tag="I9760_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC26A/SulP transporter family protein"
                     /protein_id="HAT5041939.1"
                     /translation="MTRSQLILAILAGLVISIDNMISIVAFSSLIYQGALEIYIPLVI
                     NILAISVIIISASYLFMSSPPYAIAQLQDEAAILYSAMGLVIIKSMPSSSTNEAIFIT
                     ILFMLGVTTCLTGLSFYFVGKFRFGNIIRYLPYPVICGFMAATGWLVLSGTFALLTGK
                     ALHQNWLVFFHKEEILLWAPGFIAGIVVYIFKEKLGYRYALQLMFISLVILFYALFYI
                     FNLEYLVDPTEGYVLGPFNQSHIDSLMKPEIWQMIHYPFSMTLLNYIGLVILVSFIAL
                     LLNASSYELIVNKQLDLNKELRIAGIGNVFSGLLGGMVGYLSLSASTVAKEYGGARII
                     GVMAFVPMLLILCFGAVVVECFPKMAIGCYLFYIAFCFLSEWLINTWRLLNLSEYLIV
                     LLILVIAIVFNMITAVAIGTVISIFIFAFRYSKINPVKYLFSGADYNSSFERNPISQS
                     ILSTQGDEIQFLKLQGFLFFGSIYRLLQLIKSIQKARYIILDFELVYNIDSSRIILMK
                     NLKLHAEKNNISFAICSLKPIVYHELMKTNLLQSKTNWLKVFTDQESALEWCEDQVIE
                     KYTENINIEEVTLEKQLLYLGFSSELVAWISEKASIKPYNPDDIICHEGEESNSVYFI
                     HRGRVTAFVGDKRVLVVGSGNVLGEIAIYTHKKRTATLKTNEKTIVYEIDLKSIQELS
                     KNKPLLLVQFHVCMAKILANRLSVLNKRIKVFDSTAILQ"
     gene            22230..23030
                     /gene="legP"
                     /locus_tag="I9760_000138"
     CDS             22230..23030
                     /gene="legP"
                     /locus_tag="I9760_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegP"
                     /protein_id="HAT5041940.1"
                     /translation="MKTKSWIASLFYSSLCFSSSYAINLHQITITDPIMGSRTITYEQ
                     VGEFAVVEGDILIGKAENLKNQGAVITPKVGGSRWPNGVIPYEIAENLPFINKLAILQ
                     AIDHWQKNTNLEFVELTSKNRYDYRDYISFIPAEGTTCSSYVGRQGGKQEINLAPRCT
                     TMNTVHEIGHALGMWHEQSRSDRNQYIRIVWENIDEEHKFNFDQHLTDGKDFGEYDYG
                     SIMHYGRFAFSKNGQPTIIPLVDGVEIGQRTHLSEKDIAAVKAMYPET"
     gene            complement(23027..24865)
                     /locus_tag="I9760_000139"
     CDS             complement(23027..24865)
                     /locus_tag="I9760_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg3000 family Dot/Icm T4SS effector"
                     /protein_id="HAT5041941.1"
                     /translation="MVYSKQSAAFRRDKQQFFAQIPKDSKLDTKIVTIDDRVNTIDYT
                     FLLHHKQDLEAEFTQMFTVLQQQDDENKEIFWLYCYYCASLLEAFHKAYSQNSKQAEY
                     AKIKQKIKDHLNKVNVKEEESGFIQSLYNSFLGGFRNLISSPLHASKIRDYVAYSNLC
                     RIYWTFCRLTLTNGLSVARDLKIIEKLDAILGTHTDVDKIISTLQAPTGVITYFSVGF
                     FLMRLIIDGGMLLRHTFFPTELEKGAENGCEIHQMDYLPGPASLEAYRKSYILVHEDN
                     QEVSLYYIPRSGKAEKLSIKENNKLKDALLKKMNKDTSSLRLNSTEVEEIITHQTGHV
                     PEVTTAWDRFKHEFYKRHCNFANDFVWATINLLTNYNYLFNISAPAAGMITSVFLGFD
                     VCMALYKCNLAKQEYLIKKAQYLQEIEDYNNPEKFKKMTPEQRLLHIEMLNKQLRELE
                     INYKTQEATFYFVAAAAALLMMGFTASMLVSPPILVVGCFFACTLAVAMYLSTGSYSK
                     YEEKSLYLEEAQLTGKNLPVALKEYEAARNDFIFTMVKNTVMPIVLITTFAICWPAAI
                     VLTAMYIGYEIFHAYDQHSEAKAAKQLALAAPSEEKDGYMALATPN"
     gene            complement(25161..26501)
                     /gene="mnmE"
                     /locus_tag="I9760_000140"
     CDS             complement(25161..26501)
                     /gene="mnmE"
                     /locus_tag="I9760_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015848543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAT5041942.1"
                     /translation="MSIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPR
                     LATFCSLYKGNNEVLDQGLVLYFKGPHSFTGEDVIEIQAHGSPVVLDLLIKESIAAGA
                     RLARPGEFSERAFLNDKIDLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNE
                     ELIYLRMYVEAAIDFPEEEIDFLNDGNVSQLLQKIIGRLEDIRSQANQGVLLREGLSL
                     VIAGRPNAGKSTLINNLAGRDVAIVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDS
                     DDLVEKEGIKRAWQELKRADCVLLVVDINNPDQQNSLLNELRLTLPNKIPIITVYNKI
                     DTTKLTAKCDEHTVYLSAKTGEGLDELKKVIKQVVGYQPTEGQFLARRRHLQALDEAK
                     ALLLTGQSQLTNHKAGELLAEDLRLAHQTLCEITGEFTSDDLLGKIFSSFCIGK"
     gene            complement(26502..28172)
                     /gene="yidC"
                     /locus_tag="I9760_000141"
     CDS             complement(26502..28172)
                     /gene="yidC"
                     /locus_tag="I9760_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAT5041943.1"
                     /translation="MDIRRIVLYMALALIGLSLWNAWQIDYPAKQPVEEKTASQLTSD
                     GHLLPQIIPSNAEQPVTLKAEEKASSGKQLIQVKTDVLDVDIDLKNGDIVKGLLLDYP
                     LSVEDKNKPFPLLQNQASQRYVANSSLFVLDGQTPQSLDFDFTSEKEYYELKPDQNQL
                     IVTLNGKSEDGLDVKKEFVFTKGSYLIEVNYKIANTGNSLWKGYFNTQLLRSSPKEDK
                     SSIFHIGSYTGASFSNPGKNRYQKVSFSDMSKSNLDVDAKGGWIAMQQHYFLSAWVPN
                     ADSENKFYTLATDKDYTIGAVSQPITVKPKEDKIVGSKLYIGPEITSVLKGISPSLDL
                     TVDYGILWFLSSLLFSLMKAIYTVVGNWGWSIVLVTVLIKLAFYRLSATSYKSMASMR
                     KLQPKLQALRERYGDDKAKISQATMELYKQEKVNPLGGCLPILIQIPVFIALYWVLLE
                     SVELRQAPFIFWINDLASADPYHVLPLIMGATMLIQQKLNPAPADPMQAKVMMFLPIL
                     FTGLFWNFPSGLVLYWIVNNTLSILQQWYITRKYSDEKPAKKVVATAK"
     gene            complement(28182..28427)
                     /gene="yidD"
                     /locus_tag="I9760_000142"
     CDS             complement(28182..28427)
                     /gene="yidD"
                     /locus_tag="I9760_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAT5041944.1"
                     /translation="MGKISLMLRQIVCLPIKMYQYFISPLITPCCRYYPSCSEYADSA
                     IKHYGVIKGLLMALNRLSRCHPWSKGGYDPLFPNDKN"
     gene            complement(28394..28738)
                     /gene="rnpA"
                     /locus_tag="I9760_000143"
     CDS             complement(28394..28738)
                     /gene="rnpA"
                     /locus_tag="I9760_000143"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAT5041945.1"
                     /translation="MFAFKKAQRLLKKNDFDFVFESAKKITTDDFIFLFRENKLGYAR
                     LGLALSKKMIAKAHDRNRIKRLLRESFRHTNLPAVDIIILARPGLAKKTNLGINTKLN
                     KTWEKLASCYGK"
     gene            complement(28739..28873)
                     /gene="rpmH"
                     /locus_tag="I9760_000144"
     CDS             complement(28739..28873)
                     /gene="rpmH"
                     /locus_tag="I9760_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAT5041946.1"
                     /translation="MKRTFQPSNLKRKRDHGFRLRMSTRAGRLVIKRRRAKGRKRLSA
                     "
     gene            29635..30993
                     /gene="dnaA"
                     /locus_tag="I9760_000145"
     CDS             29635..30993
                     /gene="dnaA"
                     /locus_tag="I9760_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAT5041947.1"
                     /translation="MSTTAWQKCLGLLQDEFSAQQFNTWLRPLQAYMDEQRLILLAPN
                     RFVVDWVRKHFFSRIEELIKQFSGDDIKAISIEVGSKPVEAVDTPAETIVTSSSTAPL
                     KSAPKKAVDYKSSHLNKKFVFDSFVEGNSNQLARAASMQVAERPGDAYNPLFIYGGVG
                     LGKTHLMHAIGNSILKNNPEAKVLYLHSERFVADMVKALQTNSINEFKRFYRSLNALL
                     IDDIQFFAGKDRSQEEFFHTFNALLEGQQQIILTSDRYPKEIEGMEERLKSRFGWGLT
                     VAVEPPELETRVAILISKAEQSNIELPYEVAFFIAKRIRSNVRELEGALRRVIANAHF
                     TGKPITIEFVHEALRDLLALQDKLVTIENIQKTVAEYYKVKVADLLSKRRSRSIARPR
                     QMAMALSKELTNHSLPEIGDHFGGKDHTTVIHACRKVKELIQDDSDFAEDYKNLMRIL
                     SS"
     gene            31007..32110
                     /locus_tag="I9760_000146"
     CDS             31007..32110
                     /locus_tag="I9760_000146"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAT5041948.1"
                     /translation="MFELTIKKEDLLTPLLTVSGAVDKKQSLAILSHFLLKLSDGLLY
                     ITATDLEIEMSAQVACDSKNTSGSITVPAKKFIDIIRSLDENASPSIVFDKALVTIKQ
                     NRSTFKLATLPAENYPSSEDECNDVELTIPRLVLLQLLQSTHFAMSQQDVRIFLNGLL
                     LEFDSNIISAVATDGHRMAISRYSCNNIAQTKLLIPKKGVQELLRLLNCINDEQVLLA
                     AGKSHIKLITNQFVFLSKLIEARFPPYTKAIPREQDKCIVIDCASLKRALSRIVILAH
                     EKSKAVLLHLQQGQLTLIANNNEQEEAIETLSAETQGDELKIGLNATYLLDVLTHFSE
                     GQIRLSMSNTESSILIESLVNDNYQYIIMPMKI"
     gene            32107..33168
                     /gene="recF"
                     /locus_tag="I9760_000147"
     CDS             32107..33168
                     /gene="recF"
                     /locus_tag="I9760_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAT5041949.1"
                     /translation="MILSEVRIHNFRNIASTSLILNPNFNCITGPNGGGKTSLLEALY
                     MLSCAHSFRSREITPIISYGQNQLNVFAHAYDESTISVQKSITDGTQIKLNNQFCCTT
                     SQLAYALPCQVIYSDIFQIIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNA
                     LLKSRATYDHFIPWDQQLSQLANQLDKARNDYFLQWQPKFYQVLSDLTNISCTVEYYK
                     GWDRKNAGQNMEELLQKSFDSDRKKLYTQYGPHQADLIISTEQYRVKHTLSRGQQKII
                     LIALKLAQGQLLDKDCLYLIDDLAAELDDYHQRNLIKYLTQQKGQFVITNLINNNNYD
                     ILPIDSGLFEVNCGAIKNL"
     gene            33466..35883
                     /gene="gyrB"
                     /locus_tag="I9760_000148"
     CDS             33466..35883
                     /gene="gyrB"
                     /locus_tag="I9760_000148"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAT5041950.1"
                     /translation="MSVDASYDSNNIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFE
                     VVDNSIDESLAGYCKEIFVTIHSDESITVKDDGRGIPVDIHKEEGKSAAEVIMTVLHA
                     GGKFDDNSYKVSGGLHGVGVSVVNALSEELHLLVRRHGKVYEQHYRHGVPESPIAETG
                     EANSTGTQIWFKPSAETFSNIEFHYDILAKRLRELSYLNSGVCIHLFDERTQKKDTFH
                     YEGGIKAFVEHLNKNKTPVHPVVFSMTSEKDGIVIELAMQWNDGYQETIFCFTNNIPQ
                     RDGGTHMAGFRAALTRTLNNYIENEGYAKKAKVSPTGDDAREGLTAVLSVKVPDPKFS
                     SQTKDKLVSSEVKPAVESCVAEKFNDFLLENPTAAKSIVAKIIDAARAREAARRAREM
                     TRRKGALDIAGLPGKLADCQEKDPSLSELYLVEGDSAGGSAKQARDRKFQAILPLKGK
                     ILNVEKARFDKMLSSQEVATLITALGCGIGPEEYDPDKVRYHRIIIMTDADVDGSHIR
                     TLLLTFFYRQTRELLERGYIYIAQPPLYKVKRGKQEQYVKDDEALFDYLTQCALDGAT
                     FYPAKEVPPISAKSLEELVQHFRRVEKIIKRYKRRYPSDILKRVAYMPMLHQEDFNNL
                     PKIENWCRQLHLSLQQVDSKTEQYKVDTQKLTEGNQYTPKITVYQHGLENDIPMQVEF
                     FLSKDYKEMVDLGHKLSNLIEEGAYIQRGDKSLPVENFEQVLNWLMDEAKKGQTIQRY
                     KGLGEMNPEQLWETTMDPEVRRMLQVSIEDAVAADEIFTTLMGDNVEPRRDFIETNAL
                     DAENIDI"
     gene            complement(36252..37292)
                     /locus_tag="I9760_000149"
     CDS             complement(36252..37292)
                     /locus_tag="I9760_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HAT5041951.1"
                     /translation="MNTAPKQYGFYMPAEWYPHERCWMAWPCHHETWSKIGLDKAKMA
                     YARVAKAIAQFEPVTLLVNPGDEDSAENLCKGHNIEIISLPINDSWTRDTGATFLINN
                     ERQLAGVDWIHNAWGGNYADCSLDNLIASHLIKYTEAQYFHAPLVMEGGSFHVDGEGT
                     ILTSKECLLNSNRNPHLSQQEIEQYLINYLGAERIIWLNMGLIGDETDGHVDEIATFI
                     APGKVLCLITKDKEDPNYHRLQENFEILKSSKDARGRTFEVYTVEQPPATYLNGERLT
                     LSYINFYMANQGIVMPAFGYESFDRLAYQLFVQIFPGYQITQIDALDVFSGGGGIHCI
                     TQQQPKSHKLVE"
     gene            complement(37295..39187)
                     /gene="speA"
                     /locus_tag="I9760_000150"
     CDS             complement(37295..39187)
                     /gene="speA"
                     /locus_tag="I9760_000150"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAT5041952.1"
                     /translation="MNNNTLSDDNLYNIENWGEGYFRINSKGNIEISKKPGKPGVELQ
                     TIVDAADRAGLHLPLLIRCSDILHDRVHRIYQAFTQAREDTCYAGNYQLVYPIKVNQE
                     QSVVRELLKSPDNQIGLEAGSKPELMAVIGMLGDHQSTIVCNGYKDTSYIRTALIAQQ
                     MGHKVFIVIEKKSELDIILKESARLQIKPTLGVRIRLVSKSAGKWENTGGVKSKFGLN
                     AKQVLELILQLKANDMLDCLQLMHCHLGSQIANIHDIRHCMQEVARYYIELRQLNAPI
                     GTIDVGGGLSVDYEGTRSNKGCSMNYSLVEYATHIMLALKHLCQEAEISEPDLISESG
                     RALTAHHAVLISNITDVEIINKAPVLPEISEDDSHVIRDIYDTYQAITDSSPTEIYNY
                     AAHSLNEAHSLFKHGVISLQEKAKVEAFYTNICMEIKDKLDEENPSEKALLMRINECM
                     AAKIFCNISFFQSIPDAWAIGQIFPVAPISQLNRTLSMHSILQDLTCDSDGTIKQYPG
                     SSCVTTTLMLPSYDINNPYNIGFFLVGAYQEILGNLHNLFGDTNSLDVKLSDDGQFEL
                     DDLVSGDTVTNVLNLAHFDTKKLIQSYEKQLIKSELPKEKALSYLNELRSIFSQLTYL
                     DGNIAQ"
     gene            complement(39207..40052)
                     /locus_tag="I9760_000151"
     CDS             complement(39207..40052)
                     /locus_tag="I9760_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HAT5041953.1"
                     /translation="MKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQ
                     ELTLSPYFCTRSDVDPAPYMEDIATGPTAQFVSQMAKSNQIHITASLFEKAGYNTAVA
                     FNPQGELIAVTRKQHIPSGEKYHENFYFKPGNSNYPVHTIAGHYLGLPTCYDQWFPEL
                     SRIYGLKGAEILVYPTAIGGEPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCED
                     GLSFYGSSFISTPMGEILVQAPRNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHE
                     LVKSR"
     gene            complement(40593..41852)
                     /locus_tag="I9760_000152"
     CDS             complement(40593..41852)
                     /locus_tag="I9760_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041954.1"
                     /translation="MTFTCDELKGLEHPYEVLGNGDALAENREELNKLSNDEKLVLAS
                     RLVLECPVNELKDFSHAIEAARMPQDDSDTFHSFLFQAYQVKKRIISLLDPRNKNPHS
                     MILEKEFDGELFNNFNKLAIDVLNNNEVAIALRLAETTPAQDRSRVSQNINNIFPQSL
                     FAAKVGHAFAVRRDIERLLLGDRPDQFFSSREFKIDSCIEFASLFNVINDKESSIAGK
                     LALRTPAENRTDVVMKIKGFCAEDSELAIKVQSALALRRDIERNLLGDNPEQFFNSRD
                     FSVDLCLEFAILFPELLKGHEQAIGEKLAKLDAKVRSDISRKLEMINGAAHEQSSPFR
                     IIASAMVPKEEPCLTKPTPVVLPSTEEIVPKQESPTSLRNRNVLFKEQTEEHKKKNSQ
                     MEPIVSDSEEEEEKQNCWTSFCGLFGK"
     gene            42040..42297
                     /gene="hfq"
                     /locus_tag="I9760_000153"
     CDS             42040..42297
                     /gene="hfq"
                     /locus_tag="I9760_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAT5041955.1"
                     /translation="MSKNHLLQDPFLNELRKEKVPVSVFLVNGIKLHGIIDSFDQYVV
                     MLKNSITQMVYKHAISTVVPSRMVKIPAEESSGEEEGTVAD"
     gene            42325..43569
                     /gene="hflX"
                     /locus_tag="I9760_000154"
     CDS             42325..43569
                     /gene="hflX"
                     /locus_tag="I9760_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAT5041956.1"
                     /translation="MFERPQGGERAVLVQLALPGVDADKALAEFEELALSAKAEVLDC
                     VLGARATPDAKYYVGKGKAEEIAHLVNMLDADLVLVNHELSPSQERNLERLFGCRVVD
                     RSGLILDIFAQRARTFEGKLQVELAQLQHLSTRLIRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRERIRYINKRLEKVRCSRDQNRQARRKASMPTVSLVGYTNAGKSTLFNVLT
                     GEHTYVADQLFATLDPTMRKLELPGSSAAILADTVGFIRDLPHHLVEAFRATLEETQQ
                     ADLLLHVIDISDPNWRETVFEVQKVLDELKVNNIPVIQVFNKIDLQEGWQPKIDYTED
                     SCKVWLSATTGAGLDLLKEAIATQLHGMILIEDIVIKPDQAKLRAQLYQLGAVLKESV
                     DENGDWLMKIRITADQKRRLLS"
     gene            complement(43674..44144)
                     /locus_tag="I9760_000155"
     CDS             complement(43674..44144)
                     /locus_tag="I9760_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAT5041957.1"
                     /translation="MKRLIPTFIYLLVLAITPFSTHAEALLKDTFGHNIPFSSLKGKW
                     VFINYWASWCETCVDEIPEFNRFYKNHKNEAVALYAVNYDALPLPEQKLLIKKFNINY
                     PNLLKDPAQDLQLGDITGVPVTFVFNPKGQLIKKLYGGQTAKTLEKVIAENRTS"
     gene            complement(44313..45890)
                     /gene="cegC1"
                     /locus_tag="I9760_000156"
     CDS             complement(44313..45890)
                     /gene="cegC1"
                     /locus_tag="I9760_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC1"
                     /protein_id="HAT5041958.1"
                     /translation="MNTTEHTELGNGLLMDKIEGNPYLRIDEFGVLHLRLQRFGENNL
                     PEPMELEMSAGEIIAMAGDYFTQANWTMDLDLPKCELFNSPAELGKHLIRKPIDPKEE
                     NALITAYNNLAAPDVTRKEIDRIYSINNANYVPFSPTLNFYAQQLMYYFRVKDYGEML
                     VRNQTHFTPWSIRVYILGHAIALRYARLSYELKQLATDRNYQSDNPDLSTLKISLQNK
                     NETLSSNTLLDLANRYHAQAYSIELFTFHYYSDHFATGHMSMIGDLRVVLKERFGVWG
                     NILANNLHDEVNRVGVYTVRPYDPTPNTTEAPSRARGDGKFDTCLNQFNRLACLNGMT
                     ASLKDINQVLSGGVIPEQKKFGGLVHMPDVDFNSRQHQPLLVLSKGKVYYRNNLSQIH
                     IISPSEYEALRENPEKHGYKELTSKWAAFKLVTKLRLFPYLYDGSVMPVSDEKLAEII
                     ADEKRRNPQRAPIPTPSCLPESEPTVFDWRTKASWRNNKDSLDILDGLKKHSILAAKQ
                     THSAIQEEEEVRLNLGV"
     gene            46036..46875
                     /locus_tag="I9760_000157"
     CDS             46036..46875
                     /locus_tag="I9760_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAT5041959.1"
                     /translation="MKAMEVEQLIQGTLIRDGGGVKLRRYIGADKTNHFEPILLFDYF
                     DSSDPLDYLAGFPPHPHRGFETITYLMEGSITHEDNKGNKGVINAGGVQWMTAGKGII
                     HSEMPSSSTDRLHGLQLWLNLPAAEKWREPRYQEKSSDQLPIEVMESGATIKVIAGRI
                     DQGLSSPIVEIATRPLFLDITLPANATIQQTIPDDYQAVLFVIEGTVNTGGQQITAGT
                     LASLTNGERIQIEGKDKENRNHCLLIAAAKLHEPIVRYGPFVMNTQEEIMQAIHDFRT
                     GHF"
BASE COUNT        14483 a         9279 c         8548 g        14760 t
ORIGIN      
        1 tggcctcttg cataacctaa ttattttggc gcaaacattt taaggagcga agattacgta
       61 cagcaaatga gcgccgcaga aacgtttgca acgctgcaat aaaacgaaag agactgggtt
      121 aggcaagaag tctattgaac taagcctcgt cttttaagct ttgaatacct ggaataatta
      181 tcttatccag taggcgttct ataaaaaatc atcaatcttc cttagcaact ctcgttgctt
      241 caagtagctt gaactgaagt caaactactt aaaactagct tttatgcatc ggacaaacga
      301 cccccacctc ggcaaatgcc tcgataacag gtgttttatc ccaatgctta tctatggttg
      361 cctgaatgac gccacatgca gcaaaatcat agtatgcaat aggagaccaa taattcatat
      421 tggcgtcaac cataacctga aatgcttttt gtatagacca gcctggttta tgtgctaata
      481 aataaaatgc tttattaaat actccgctcg ttatatgcac atctaacgca tcagtgtatt
      541 cactagcgtg ccctatagac attccatcct tggctggatc atccaaataa cgaataggtt
      601 gcccactttt tacagcttca cgcccaatgg tccaatcctc accatcccaa taccatggat
      661 aatctttact aagataatct aacaatgcaa ttgccgccat atcagagaaa gattcattga
      721 tccccccaga ttgcccttca tacatcaatc cggaatgcaa ggcagtaaaa ttgtgcgata
      781 attcatgtcc caatacactc tgggcgggag ctgttaaaaa ttcatcacca tttccgatta
      841 caatttgctg atgtgcaaga actaccccat caagactgat agttggtact gcaaaagcgt
      901 tatccatgtc accaagatgg gtgtaagccc gtataggtaa atcatcacct attggacgat
      961 taacaccata aactttttga tacatatcta acgtttgttg ggcaaaatac atcgtatcgt
     1021 taactggaga aaaagcataa ttgacaggac ctgttctgcc atcagcatag tttaaaaaca
     1081 aattggtttc attacatggg taggaaaagg cacttaaatt cagtacagac tctgcaaata
     1141 tggtaattgg aaagaaatca taacctatat tatgattttc caaattcatt acttttatgg
     1201 attcattttc aacacgacat aagccgtcct caacattaac gtcaaacttc cctaatgagg
     1261 gaagccccgg caaagcatta ccatgctgaa acattccctg acggtatggg agagtaaacg
     1321 cattaccgcc taatccctga ccaattcttt catgacgcac ctcattccat tgtttcaaaa
     1381 tctcaccgct gttggcatca ataagataat taggtgcatt cacatgtttt ttttcactat
     1441 tggaatagta agacaaatga taagccaaat gagccttatt tcctttatct atataaataa
     1501 cctttttaag gtttttaaat ttaaccggtt cagttacctt tccaagagtc ttttgttcta
     1561 tatcattcgg gctcaatttt ccctcagtag aattaatatc ctgttcaatt ccggtaatat
     1621 tcatacctgt tacagtttct ttggcgccat ctttggaatg aaaaatgact tgataacccc
     1681 aaaaccggaa cccccttgta ggttatttga taacgagcat gcctggtcgc atggttagaa
     1741 ttattttgta actccagttt ataatcactt tgctgacttt ttacagtttg caataatcct
     1801 tgtttcttaa aaggatgtgc ttgcataaat tgaggtaatg ctttcgttga ctcaccccat
     1861 acgattgctt gggtagctgc gtttacagat gaaatagctg ttgaaatgac aaataatgaa
     1921 acaatccgtt ttttcataat atcatcctga ttttttgtag aaatttcatg ctgctacaaa
     1981 ttcgagaaaa aaaaacaatc agaagacaca aattacgaca aaattttatt tttttatatc
     2041 tagaataaca atttatactc aacttcttat cgcaaacgat tccctcagac tctgctaatg
     2101 tccgcttaat tcagactgat aatttagcaa gacaataaat tattttccat tataataaga
     2161 aaatgtactg agacagacat tggtttcact atgaatttca atattattgg tgctgggcgt
     2221 ctgggtaaga atttaggcat tgcaatgtct gttcatcaga ttgcctcttt aaattctatt
     2281 tgtaactcaa atttagcaag tgccaagata gcctgcaatg aaatcggatt tggcaatgcg
     2341 gttggtaaaa tatctgatct ttctgaagca gatataacgt ggataacctg tagggacgac
     2401 tccattttgg atgtagtatc gatattggac aatattgaga tattaaaaaa aggcagcttg
     2461 gtggtccact gcagcggcgc tcttaattca agtgtacttc ttcctctgaa aaaacagggt
     2521 tgttatattg caagcgcgca tcctttgaaa gcattccgca ctaattcttt ggattccaat
     2581 gcgtttaaac aagtccattg cgtgatagag ggagattatg aggcatgtcg ttggcttagg
     2641 tttgcatttg agaagctgga agccagcatt atcgcaattc aacctgaaat gaaggtcatt
     2701 tatcatactg cagcaacgat tgcttctaat tatttagtga ctttagcttc atatagtgaa
     2761 gagttacttt taaaagcggg aatacagcaa gcacaatcaa gaaaaatgat atgtgatctc
     2821 atgtacagca acatccttaa cttaagtcag gtagatagcg caaaaagtgc tttaaccgga
     2881 cctttgacta gaggagatat gcaaactatt tccttgcatc tcgaagcgat agataatagt
     2941 gaaatcaaga gattatataa aagcatggcg ctagctaccc tgcctttgtt agatttatca
     3001 ttagatagaa aagaaacctt aagaaaaact cttgaaaaag aataacgttg cagttattga
     3061 acaataagcc agctctgtgc actattctgt ttgatttcaa atcatcactt aaaggtgttt
     3121 cacgggaaac atacagtccc taacggccat gttactcaga ttgagtaaca tgcaattgag
     3181 aaacattttg taaacctctt ggcaaccggt ttcctctcct gcccctttca cccttatagt
     3241 gagtcaaatc ctcacctttc agagtaaaat gccgtttacc agcataaacc gtcagcgaat
     3301 cattcgttgt taaaacttgc atatcaacta tatactcttc tcgtgagacc gctttagcga
     3361 cagggatact gatcaattta ttcccttttc ctcgagataa ttcaggcaac tcctcaagag
     3421 aaaaaatcaa taaacgccct acattagtgg cacaagcaac aaataattgc tctcgatttg
     3481 gtattagcct tggcggcaat atatgactgt tttcacttaa tttgatgcat gcctttccat
     3541 tacgattttt tacatataaa tcttcaattt tggcgataaa tccatacccg gaatcggtag
     3601 ccaataacac cttttgttca ggctctccgc caacaattgc ttcaaacaat actccttcag
     3661 cagggtttaa ttttccggtc aaaggctcgc cttgaccacg tgccgatggc aatacatgtc
     3721 ctggaagcga atagaccttt ccctctccat caaaaaataa aacttgctga ttagtacgag
     3781 ctagcgtttg ggctttgaat tcatctcccg ctttatagct gagttcctta ccatcgacat
     3841 catgcccctt ggccgctctg acccatccat ttttagacaa tacaaccgta ataggctcat
     3901 taggcaaaat atcctcttct tttaatgcct gagagtcttc ccttgtaata atgggtgaaa
     3961 ggcgtgggtc accaaactca tctcgatctt tcgttatctc ttcttttacc agttctttta
     4021 agagagaatg actagccagt gttttttgta aagtatcacg ttcatcagac agctcatcca
     4081 gttcaccacg caatttaatc tcttccaatt tggctaaatg ccgcagtttc atttccaaaa
     4141 ttgcttcagc ctgtctttcg gttaagttaa agcgtgaaat taatccttgt tttggttgat
     4201 catgttctct gatgattgca atgacttcat ctatattaag ataggcaata agcagtcctt
     4261 caagaatatg aatacgctcc agtacctttt ccaatctgaa ttgaagacgt ctttttactg
     4321 tggcaagcct aaattccaac cattcagtca agattgctaa cagtccttta actcttggct
     4381 tgccatcaag cccaatcata ttaaaattaa ctcgatagct acgctccaaa tccgtggtag
     4441 caaataaatg cgacattaaa ctttctatat ctactcgatt agagcgaggg ataataacca
     4501 accgtgttgg atgctcgtga tctgattcat cacgtaaatc ctcaaccatc ggtaattttt
     4561 tctgttgcat ctgcaacgcg atttgctcca ttaccctggc tccggatact tgataaggca
     4621 aagctgttat gacgatgttt tgtttttctt gagtataaat tgctcgcatt ttaattgagc
     4681 cattaccgga ctcgtatata gctctaattg tttcacttgg gctaatgatt tctgcttctg
     4741 ttggaaaatc agggccttta atatattcac aaatagtctt aatatctgct tgggggttat
     4801 ccagcagatg aatacacgca ttagcaactt cagtcaaatt gtgcggaaga atatctgaag
     4861 ccataccaac agctatacct gttgcaccat tcaataaaat attaggcaaa cgagctggta
     4921 aaagagcggg ctcttgcaaa gtgccatcaa aattatctac ccaatctaca gttccttgta
     4981 gcagttctcc gagtaacaca tcagcataat gagataatct tgcttctgta tacctcattg
     5041 cagcaaatga tttgggatca tccggggatc cccaatttcc ttgtccatca acaaatggat
     5101 agcgatatga aaagggttga gccattaaaa ccatagcctc atagcatgca ctatcaccat
     5161 gaggatggaa tttaccaaga acatccccta ctgtacgcgc tgatttttta tattttgctg
     5221 ttgctttcaa acccaactca gacatagcat aaacaattct gcgttgaaca ggcttaagtc
     5281 catctgcgat gctcggtaat gctctgtcca aaatgacata cattgaataa tcaagataag
     5341 ctttttctgt aaactcagta agcggttttt tttcagttac ttcattcata ttgggtattt
     5401 tcttcatata ttaattgcta tccatcatcc tagtgaatcg tataagacaa tgcaaaacaa
     5461 ttttaacaga atattcatgc tgttagaatt ataatcatcg tattatctgt gtataacttt
     5521 atattctttt tgctttatct atatggatta agtttaactt attgatttat attaacattt
     5581 attaatttca tttaatttta tatctttgtg tcctgtggat aactatgtga atgaatatgg
     5641 aataaataag ataacttgtt gttaattacc aagaattttt tataagttgt aaattgttct
     5701 ctccactata aacaaatttt caaatttcag ttgttaccac ttgattttta aatgaccaat
     5761 tgcccctggt attgcctttc aaccataatg gcaatgtaca tactggatac catatctatg
     5821 catccgcaga taacttgaat tccctcaaat aatagagcaa tgcaaagcat tagagttagc
     5881 acactatttt ctggtgtatt agagagaaag atacggcgac aaaattacat ttactaagag
     5941 agtaaaacac gggtttttcc tatcgggaag aaacccgttt tgtagaggag tataaggtat
     6001 tcatagcaaa gcagtttcca caaacaggaa actcttactt catttttttc ctggtctttt
     6061 gaattgctct tatttgagcg acagccctgg ccaattctgc tgcagcaaca gagtaatcaa
     6121 tatcgcctcc tttactggct atagcggctt cagctttcgc tttagctgcc aatgcagcag
     6181 cttcatccaa gtgctcggct cgttctgcca catcagcaag aattgttaca cagtgaggct
     6241 gaacctcaag cattccaccc tgcacatagt atatgtcctg tgtaccacct tgcaaagtaa
     6301 ttctaacttc acccggcctt agcacagtta gtaatggagc atgccctgga gtaattccaa
     6361 tttcacccaa ctctccagtt gcaactacca tttctaccac accagagaaa atttcatgct
     6421 ctgcactaac aatatccaag tgcgttgtaa tactcatatt atcttgcctc ataaggtttt
     6481 agcttttgca acagcttcct cgatgcttcc aaccatataa aatgcctgtt caggcaaatc
     6541 atcatattca ccagcaagaa tgccttggaa acctttgatg gtatctttaa gcgatacgta
     6601 tttaccagga gaacctgtaa atacttccgc aacgaaaaat ggctgagata agaatctttg
     6661 gattttacga gctcgagtta caactcgttt gtcttcctca gacagttcat ccatacccaa
     6721 aattgcaata atatctttta attctttata tcgttgcaag gtttgctgta cacgacgtgc
     6781 ggtatcgtaa tgctcttgac ctacaatcaa tggatccagc tgacgtgatg tagaatcaag
     6841 cggatcaacc gcaggataaa tacctaattc agcaatttga cgagacaata ctacagtggc
     6901 atccaagtgt gcaaaggttg tagctggtga tgggtcagtc aagtcgtctg caggtacata
     6961 tacagcttgt atggaagtaa tagaacctgt ttttgtggag gtaatgcgct cttgcagcat
     7021 acccatttcc tctgctaatg tcggctgata tcctactgca gaaggcatac ggcctaacag
     7081 cgcagatact tcaaccccag ccaaggtata acgataaata ttatcgataa acaaaagaac
     7141 gtctcgccct tcatcccgga atttttcagc catagtcaaa ccggttagag caacacgcaa
     7201 acggtttcct ggcggctcat tcatctgacc ataaacaagc gatactttat ccaatacatt
     7261 agagtctttc atctcatgat agaagtcgtt tccttcacgg gtacgttcac caacccctgc
     7321 aaacactgaa taaccgctat gctcaattgc aatgtttcgt attaattcca tcatgttaac
     7381 ggttttgcct acaccggcac caccgaatag accaacttta cctcccttgg caaaagggca
     7441 aagcaaatca attactttaa taccagtttc caataattcc tggctgccag cttgctcttc
     7501 ataacttggt gctttacgat gaatagccca agtctcttca gcatcgatag gcccagcatc
     7561 atctacagga cgcccaagaa catccataat gcgtcccaaa gttttcttac ctactggcac
     7621 ttgaatagga tggcctgtat tttctgcttt taatcctcgc tttaaaccat cggttgttcc
     7681 catggcaata gtacgcacaa ctccgtctcc aagttgctgc tgcacttcaa aaaccagatc
     7741 actatcaaca agctttaacg catcattgac tttaggcacg ctatcacgag gaaattctac
     7801 gtcaactacc gcaccaatta cttctaccac agttcctaag ctcattatat accctcttta
     7861 taaagcggct gcgccaccga caatttctgc caactcttga gtaatggctg cttgtcgggc
     7921 tttgttataa gccaattgaa attctttaat caaatcacca gcattatccg ttgcactttt
     7981 cattgctatc atctttgcag cttgttcaca agcaatgttt tctacaacag cttgatacac
     8041 ttgtaattca atatatcgct ctaaaatttc atctaataat tctttagctt cgggctcgta
     8101 aatatagtcc caatgatgac ccaatgtttt tttatcttct tctgattttg gtaacggcaa
     8161 taattgtttt acaaaaggtt tttgcgtcat tgtattaatg aactcattgt atacaatgtg
     8221 caaagaatca atggtgccat tgtaataagc atcaagcatt atttttacaa caccaatgaa
     8281 atcattgata ctcggtgtat cacccagatg atctatggaa cctaatatgt tcccacccac
     8341 acgcctaaaa aaggcctggc cctttcttcc gataacagcg atatcaactt ccttgccatg
     8401 ttcttgccaa ttacgaatag tacgaattgt ttcacgaaat agattcgagt ttaaaccacc
     8461 acaaagacct ctatctgtgg tgacaacaat aatacctata cgcttgattt cgcgctcact
     8521 cataaatggg tgtctgtact cagatgctgc tcgagcaata tgctttatga cttcatagat
     8581 tttattcgca taaggtttag aagcgcgcat tctttcttgc gttttgcgca ttttgcttgc
     8641 agccaccatt tccatagctc gagtaatttt tcgagtttta ttaatactcg aaatttttga
     8701 acgtatttct tttgctccag ccatctttca attcgcctta attagtttta gaggccatta
     8761 ctttcaaata atggcctttg tgcctcaaag agttagctct ttgaggccat accttaccaa
     8821 ctgcctgtgt tcttgaactc ttctactgct tttttcagtt ttgcttcgat ttcattatca
     8881 taagcaccgg cttcattaat tgcatgtaat aaagcggcat gggtactgcg catgtaatcg
     8941 tgcaaggaag cttcaaacga gctgatttca tttacaggta catcgtccaa gtatcccttt
     9001 tctaccacaa acaagcttac ccccatttca gccactgtaa gtggggagta ttgcttttgt
     9061 ttcatcagct ctgtaatgcg ttgtccacgc tccagctgct tacgtgttgc atcatcaaga
     9121 tcagatgcaa attgggagaa tgcttctaac tcacggaatt gtgccagagc aagccgagta
     9181 ccaccaccaa gctttttcat gattttcgtt tgtgcagcac caccaacccg tgaaacggac
     9241 agtccggaat tgattgctgg tctaacacct gagttaaata agtcaacatc aaggaatatt
     9301 tgcccatcgg taatagagat tacgtttgtt ggtacgaagg ctgacacatc tcccgcttgt
     9361 gtttcgataa tcggcaatgc agtcagtgat cctgttttac ccttcacttc tccattagtt
     9421 aatttttcta cttcatcggc attaattctt gcggctcttt ccaacaagcg agaatgcaaa
     9481 tagaaaatat caccaggata agcttcgcga cctggtggcc gacgtaataa caaggagatc
     9541 tgcctatatg cccatgcttg tttagttaaa tcatcgtaaa caatcaatgc atcttcccca
     9601 cgctccatga aatactcacc catggtacaa cctgagtaag gtgctatata ttggagtgct
     9661 gctgaatcag aggctcccgc taccacaaca atagtatgct caagagcgcc atgctcttcc
     9721 aattttctaa caatcgatgc aacggatgaa gccttttgac caatggctac atatacgcat
     9781 ttaacgccgg tacctttttg attgataatc gcatcaatag caatggcagt tttaccagtt
     9841 tggcggtcac caataataag ctctcgctga cctcttccaa cggggatcat cgcatcgatt
     9901 gcttttaatc ctgtctgaac aggttgatct actgatttac gtgtaattac accaggagcg
     9961 actttttcta ttggagacat acctgatgac tctatcgggc ctttcccgtc tataggattt
    10021 cctaaagcat caacgacacg tcccaaaaga ccttttccaa ctgggacttc cagaatacga
    10081 cctgtacatt tacctttttg cccttcagct aatgtggatg agtcgcctaa tattacagca
    10141 ccaacagaat ctctctccag gttaagagcc aatccgtaga cccctcctgg aaactctatc
    10201 atttcacctg ccataacatc ggcaagacca tgcagtcgta caataccgtc tttcaaacta
    10261 acgatagtac cttcatttcg tgcttcagaa accacactaa attgatctat tttctttctg
    10321 attaattcac tgatttcaga aggatttaac gctacttgtt ctgacatgga aactatcctc
    10381 taaattaagc ggccaagctg gtgccaagca tattaagctt gccccgaact gaaccatcga
    10441 taactaaatc gcctgctcga attagcgctc cgccaagcaa ggagggatca atacttattt
    10501 ttaaagaaac cttacgtgat aatctttgac ttaaggattc gctcaaacgc tgttgttgcg
    10561 ctggagtcaa ttcggaatag ctgactacat caacctccag tattttttct tgctctgctc
    10621 tatatacttc ataaagagct tcaatttctg gcaacaacat caatctttta ttggttgtta
    10681 atagcttgat taaattattt agtgcatcat ttttcttgaa ctttgaacca catacctcaa
    10741 taagaagttc aatttgttgt tcatctgttg atgcaggatt agcgataaac tgagtagctt
    10801 gaggagttaa aacaacttga gccagtaatg taagatattc tgaccattca ctcagttttt
    10861 tttctgctaa agcatgttca aatatcgctt ttgcataggg tctggctata gtggtactat
    10921 cagacatttt aaatctcttc aattaggtta tctaacagtt cgctattcgc ttttgcatct
    10981 acctctcgca ttaaaatttt ctctgcccca gtaatagcca gctttgcaac ttgttttctc
    11041 agttcttctt tggcgtgatt tacttgttga gcaatttgtt cttgcgcgag ttttgcttga
    11101 atttgagctt cgcgttttgc ggcctctttt gctgcttcaa taatctctga tgctcgctta
    11161 ttcgctttat caataatatc agcagagtga gccttggctt gttttaactc atcttttaca
    11221 cgatgctgag ccaactccaa ctctttgcgc cctctttcag cagcagccaa tccatcagca
    11281 attttatctt gtctttcttc aagtgctttt gctagtggag gccaaactaa tttcatagtg
    11341 aatagtacaa acgcggcaaa aaccaacatt tgaactatta atgttaaatt aatatccaaa
    11401 gtatattctc ctgtaacagt gagggcgcat agcgccctat ttgtgtgtta tcaagaaccc
    11461 aggctgctta ggaaagggtt agcaaaggta aagaataatg caatacctac cccaatcatg
    11521 gttaccgcat caagcaaacc agctacaata aacattttaa cttgtaacat aggtaccatt
    11581 tctggttgac gagctgagcc ttcgagaaat ttaccgccta gcaatccgaa acctatggct
    11641 gtgcctaacg cacctaaacc tatcaacaag gcaaccgcaa tgacggtcat actttgaact
    11701 tgtgctatta attcagcagc ttgcatattt tatccccttt acaatggatg atgataatta
    11761 aatcggttaa tgatcttcat gggccaaact caaataaact atagtcagta ccataaaaat
    11821 aaatgcttgt aaggttatca ctaatatatg gaaaatggac caggcgagtg caagaataaa
    11881 ttgcgccgta cccagtgtca tagtactaat taatgatgac gttgttgcat ttaaggttag
    11941 taaggctatc aaaataaata ttaattcgcc agcgtataag ttaccaaaaa gtcgtaatgc
    12001 caaggaaatg ggtttagcta tcagcccaac caactccaac aataaattaa atggaatgaa
    12061 tagtgggtga ttgaagggct gcagggtgag ctctttaaca aaacctttaa cgccttttat
    12121 ttttaaactg taaaaaacta ttagaaaaaa tacagaaagt gacaatccaa atgttaaatt
    12181 caagtcatta gttggtacaa ctttcaggta gtgtataccg actagctgcg caagtacagg
    12241 aaggatatct accggcacga tatccataaa attcatcagg aatacccaca tgaagatagt
    12301 taatgccaat gggcctatta ggttattttt tccatgaaaa caatctttaa cttgattatc
    12361 agcaaactcg agcatgagct cagcaaaatt ttgtaacttt ccaggaacac cagtggttac
    12421 tttccttgcc gctaaataca taattgccag agcaattaga cccaaggcta tagaaaagaa
    12481 gattgtatcc aggttcaaag tccaaaaacc accacttgaa ccgaatgtca tatcatttac
    12541 gttataggta aggtacgtta aatgatgctt aatatagttt gtgctagata ccatttcagt
    12601 cactttttgg cctattctgt ttattgacaa taatccatgg ggcaaaccaa tgcgtcataa
    12661 gaaccaaaat gtatgatgca aaaaacgctg ccggtgtgat cctacataat aaaaatacta
    12721 atgcaaacag gaacatggac aatattattt ttaacgcttc acccttataa aaactattaa
    12781 ctatttgcct cgcagctctc gcgccttgat gcttaaacag tttaatagca aagtaagcat
    12841 ttggtataat acaaaccagt ccaccaagca atgcagaact ggccgcattg acaccaaata
    12901 aaattgcgca aaggccagcc aggattatag taactcccaa ctgcgccagc cacaatcgcg
    12961 tgattccgcg cttattcagc tgtttgttca catattcacc taactttcac ggcgcgaatt
    13021 ataaattaaa caattgtaat aatcaacgtt gatattcaaa ttacgagcta taattgtaat
    13081 gtagggttta gcattaatca catgttaagc gatttactat ttaacctcgc ctaaggatta
    13141 aaaaatgtca gagaaaattc agcatacagc agccaggctg caagaaaaaa tgagcaatcg
    13201 ttatagcgat gggctttcac acaagcataa taaaaatcac ctttcatatc gacaacattg
    13261 cagatctcat ccatgcaatc aaacgaacaa taacgacgaa gaaaatattg taaatggctc
    13321 tacagggctg ttacgtgagt ttaatttgag aaaaatacga aaacgactgg ataaaggtcg
    13381 attccattga tacagtatac ttatcctccc aggttagcca cttcctggat tggacgtgca
    13441 gctattggcc tgattctgat caggccatat tcgtactggt ttgataagta taataccgca
    13501 taagttttat tactttctgt acaccacctg tgtatcttgc aattttaatc actttttcag
    13561 cttgttctgt ctgaacatct cccatcaaat agacaacacg atcagaagta accaccttga
    13621 aagcgttagg atcgattgaa gagtctgcaa aaatttggct acggattttc gtggtaatcc
    13681 agctgtcctg gatcgagttg gatggcattt tattgataat tacacgattg aataaccgcc
    13741 tgtatccttt aactttgctt agacgtagtt gtaattcatc gtacaactcc tgggttggaa
    13801 catgccctgc tattaaaata tctctgttga acacagcaat atccagtact gtctctgagt
    13861 ttttaaatgt ccggttgaca gctaaaacat cattaagagc ttttattaaa ttataatcat
    13921 ctaatttttt atatacattg tgccggtcat aggctagagt tgccccagtc cataaaaccc
    13981 ccagacagcc tgacaagcag ctggctaaaa aaaagactat tagtataaaa catccgtgtt
    14041 taaacatgct aacgtatata ttggaatatt tttacgactt ttctcactcc agcaacacgt
    14101 cttgccacat caacagctag caatgcttgc tcgtcagaaa caattcccat cagatagact
    14161 actccatttt cagtgactat ccgtattgaa cccgattcaa gtcccttttt agtcaacatg
    14221 ttacttctca cttgacttgt aatccaactg tcttttgtcc tttgagtcaa gggaatggga
    14281 ttatcaacgg ttatttcatc atacactctt ttaactccag gggcattttg ggcaatccgc
    14341 tctgctagaa cgcgtaatga tgcagcggga gtttgtccaa ccagcaatac cacccggtta
    14401 aaactggaaa caagaattct cgagtgccga aatcgagggt ccttgatgat ggttttatga
    14461 atcacatgaa ataatcgggc gtccgcctcc atggtagtta cgcttcttct atcataaacc
    14521 attcctgctg cagcacctgc aacaaccaca gcaacgcaac cagtcagtaa agcaccaatc
    14581 atcattacta tgatggattt aaattttaga ttcatgttca ttttttatcc taaaacttgg
    14641 ccaaataaag attgttcgat taaatcacaa aaacaatgca atatgaacaa gtgtagctct
    14701 cttatcaatg cagtactgtc agatgccact cttatctcta tatcctctgg ccccatatga
    14761 ttcgccaaaa cacctccatc gcggccactt agtgcaatgg tatccatgcc gcgttcactt
    14821 gctgcgtgaa gagcttgcag catgctatca gaattacctg atgtagttag taaaagtaat
    14881 acatcttgct cttggcccaa ggcttgaatt tgccttgcaa acacttgacc aaagtgatgc
    14941 tcatcggcaa atgaactcaa acaggacgca tcagcgcata aattaatgac cggcaaagaa
    15001 ggtctttcca cttcaaaatg atttagcatg gaactggaaa aatgcatgca attagcacta
    15061 gatcccccac tggcgcaaat gaatattttt ccatcattca ataaacagtt aaccaacaac
    15121 ttccctgctt tagctattga acccgacaac acatcagctg ctgatatttt tgcttctatt
    15181 gtgacaccaa ataaatgcct tactctttct tctaactgaa tcatttcaaa accttctatt
    15241 cattatcttc ctagcatcct gcatcaaatg cgttctttaa ccaggtaatc ttgtttgatt
    15301 ttccatctat actaatcaca tcaaaccgta taggaaattt atcttgtatg cgatacttaa
    15361 tcatataatg agaagttgct tttattattt tttgtttctt ttcgtaagta atgctggcaa
    15421 gccccccacc aaagttcata tttgaacgcg aacggacctc aacaaaaacc agataagatc
    15481 cctcacgcat aattaaatca atttctccta aacggcaatg atagttttgc attactaatg
    15541 caagaccatt ttcctttaag taatttaaag ccaattgttc agcaaacttt cctttttctt
    15601 gggtcattat tcttggctca cttcaaaact aaacagtatc ccctagtgaa tgggctaaac
    15661 cctttttaaa ttgcccccat tcaagaaccc tggctacttg ttgagtcggc tttaaatata
    15721 aaatacctgt tgaatcgtta gagccatcgg ctggaaataa tatcagttgg tttaattggg
    15781 tagccaaggt ataactgtcc acgcctaaag cataaagcct gttatagcta ttgaattgtt
    15841 caggccagtt tttcgttccc atctgatgag aaaataccca aggcatatcg cagaaaatga
    15901 tcccatccaa atccttatcc ttcaaagcat tggcgctacc gccataaaca cttgaagtag
    15961 cgtacacagg agcatctccg gcataataat attttaataa aggcataatt tggcgggcct
    16021 tggaaggata agccaataaa aatatcatat caaaatcctg tcttcggctc gtagtcgatt
    16081 ggaaattata gcctaatacc tgctttagtt ttttctcgcg ctcctggcta ttggtgattt
    16141 gtaaaaaatc tttcatgctc ttattcaaat cttgtttggc ttcgtagcga agagagtcaa
    16201 caatatggcc cccatcttca ttccactgat tgataaaagc tttcgttact tcattacccc
    16261 aagcattatt gggggcaata atcagcgctt ttttatatcc tttgcttctt gcttttattg
    16321 caacctgaat cgcttcatta acaggggata agcctaagga ataagaattg ctttgtgtgc
    16381 tggtatccgt atcgtttagc aatagggtag gaaccggatg ctccattgat gcaatggtag
    16441 cgacttgagt tttcgttaaa ggaccaacaa catattcggc accgtcagaa atagcttggc
    16501 gatatgtgtt cgttatatcg tctttgcttg tatcataaac tttaattttt gttgattcat
    16561 ctcccctgtt ggccttatag gctgccataa agccttcccg tatagcatta cccggcccgg
    16621 ataatggacc acttaaaggc aataacaaag caacctgttt aggtggagct aataatttac
    16681 ctgatatgct gtctaaggga ttaggcaaaa tctgatttgc tggatgatta ctaaaatgca
    16741 tctgccattg atccaaggca gccagcaacg actttgagtt atttctatac ttacgggaaa
    16801 taacagccag ctgcaaccag ccttgcattt ctgatttgtt cacagcctcc agagccattg
    16861 tattcagctc ctcttgaggt aaactggtta aggtcaacca caaagcccgc cgattattga
    16921 cctgattttc ctcatcaggg agcaatgatt ctaacttgat acgctcacta actgattcag
    16981 agtaactacc aatggaccga aatgacttcg ccaattgttc gtgaaattgg atttgattat
    17041 ataacgagag tctttcaggt tctttaattc tagctaactt ggctaaggca tctctgggcc
    17101 tatcacgcat aatgtcgact tttgccagta aaagattctt ttcattagct tgctcaactg
    17161 ttaattcccc agtttgtgct aaaatggcag ttccttgctt ccattgacca tcagtaatta
    17221 aacggccagc tgcggaaatc aatgcgcttt gcttttcatg gccttgttga ttcttagcca
    17281 tagccaagta tgctgcagta ggtaatgaat atggagattt taatttttta ttaactgtta
    17341 ctacaggctc ggttgagtta actgtttttg tgcattggca aagcaaaaaa agagatacca
    17401 atactaagaa catgcgaagt tttaattgtt taattaacat gttaattcgc caaatttgct
    17461 tttcgcaaga ggataaacta tgacaaacac tctagcaaca ggtttaggaa ctctctatat
    17521 agttgctacc ccaataggaa atcgcgaaga tatttctttc agggctctta ataccttgaa
    17581 aagcgttgat ttaatacttg ctgaagacac acgccattcc atgcaattat tgacttcgct
    17641 agggataaaa aataatttag cttcattgca cacccacaat gaagcgaaca aaagcaatga
    17701 gataattgag ctgctgctcc atggaaaatc aatcgcacta atcagtgatg caggcacgcc
    17761 cttgattagc gatcctggat ttcctttagt gaaacaggct cgccagcacc atatccctgt
    17821 tgtgcctgtt cctggcgcat gcgctttaat tgctgcgtta agtgctgccg gggtaccctg
    17881 cgattctttt gcctttttag gttttcttcc ggccaaacaa agtgccagaa aacatgccct
    17941 ggaatcatcg cggtcagtgc cttataccat agtattctat gaatcaactc acagaatcat
    18001 agactgtctt aacgatattg cagagatcta tggccaagat tatgaacttg ttttagcaaa
    18061 agaaataacc aaaacctttg aacgatttgt atctggaaaa ataaaagaaa ttaaagactg
    18121 gctactcagt gaacccggtc atatcaaagg tgaatttgtt ttgatttttc cacctcgctc
    18181 aacaaataaa gaccccaact cgcatgaaga actactcaag atattgttgg aagagttgcc
    18241 tttaaagcag gctgtggcta ttgcatgcag attgactaat gccaataaaa atcagctcta
    18301 tgaagaggca ttgaaattaa aagagagcta aaaagaatat tgatcgctta tttccataac
    18361 attgtccgta aatgaccttt ttttgtcctc taagaccaag caaagatatc gccttttaag
    18421 ctatattgtt tctatattag gctatgcttg gaattataaa atggatgatt taaaaaaata
    18481 tagtttttta ctggtctatt tacttatccc attacccttt gtctctttat atttaggagg
    18541 ctggtacagt tttctaccct tttttattgt ttttgctttt attccattaa ttgatgcctt
    18601 tcttcatgat tcggtaaatc caacaaaaga gcaggaacaa gaactcctaa aagataagtt
    18661 ctttaaatat attacttgcg cctatgtgcc tatacaaata accatcattg cttatggagt
    18721 ctatatagtt agtactcaaa atttggtttg gaatgaatgg ttaggattta ccatgtcact
    18781 aggacttatc tcaggtggag taggcatcaa ttttgctcat gaaatgatgc ataaaaacag
    18841 taaattgcaa caactaatga gtaaaatttt attggttaca gtttgctatg gtcatttttt
    18901 tatcgagcat gtcaaaggcc atcatgttaa agtagcaacg cctgacgatc ctgccacagc
    18961 acgattaggc gaaaatttat atcaattttt accaaaaaca ttattaggtt cgtttcaatc
    19021 tgccttaatt ttggaagaaa agcgtttatc gcataaaaat tattccatct ggagcataca
    19081 taataatttt tggtggatcc ttactgtgcc attaatgatt tgtatagtct gtttcatttt
    19141 tgggggatta atcgctctgt catttttctt attacaatca ttcattgcca tcctgacatt
    19201 agaaattgtt aattatattg agcactatgg gctggaaagg aaaatgctga gtaacggcca
    19261 atatgaaaag gtaaatccaa atcattcatg gaatgctaat cattggctaa gtaatcagct
    19321 gctaattcat ttacaacgcc attcagatca ccatgcccat ggtgcacgac cctatcaaat
    19381 tttacgtcat atggagaaca gccctcaact tccctcaggg tacctcggaa tgattatcat
    19441 agctttaatt ccaccgctct ggcattacat catggacaag agagttcttg cctatcgaat
    19501 tcagttaaga caattggaac aactttaaaa cgttgttcct tattatttca cagtaaaaga
    19561 atattttatt aacttttatt gcaatatggc ggtggaatca aaaactttta tccgtttatt
    19621 tagtaccgag agacggtttg ccaaaatttt agccatacac acatgaaatt gaaccaagag
    19681 taggggttta tttttagaaa gctcctggat tgatttcaaa tcaatttcat aaacaatggt
    19741 tttttcattg gtttttaatg ttgctgtgcg cttcttatga gtgtatatag caatttcacc
    19801 aagtacatta cctgaaccca caacaagtac acgtttatca ccgacaaatg ctgtaactct
    19861 tccccgatga ataaagtaaa cactgtttga ctcttctcct tcatgacaaa tgatatcatc
    19921 agggttataa ggttttatag aagctttttc actaatccat gctaccagtt cagaggaaaa
    19981 acctaaatag agtaactgtt tctcaagtgt tacttcttca atatttatat tttctgtata
    20041 tttttctata acttgatctt cacaccactc taaagctgat tcttgatcag taaagacttt
    20101 aagccagttg gttttcgatt gtaataaatt ggttttcatt aattcatgat aaacaatggg
    20161 ctttaatgaa caaattgcaa aggaaatatt attcttttcc gcatggagct ttaagttttt
    20221 cattaaaata atgcgggatg agtcgatatt gtagacaagc tcaaagtcta aaataatata
    20281 tcgagctttc tgtatacttt ttattaactg aagtagcctg taaatagagc caaaaaaaag
    20341 aaatccttgc aacttcaaaa actgaatttc atctccctgt gtcgataaaa tggattgaga
    20401 tataggattt ctctcaaaag aagaattata atctgctccg gaaaacaaat acttaaccgg
    20461 atttattttg ctgtagcgaa aagcgaagat aaaaattgaa ataaccgttc ctatagcaac
    20521 cgccgtaatc atattaaaga caatagcaat aaccaatatc agcaaaacga ttaaatattc
    20581 acttaaattt aataaacgcc aggtattgat caaccattca gatagaaagc aaaaagcgat
    20641 ataaaacaaa taacagccga tagccatttt cgggaaacat tcaacgacca cagcaccaaa
    20701 gcacaaaata agcaacatgg gaacgaaagc catcacccct ataatacgag cgccgccata
    20761 ctcttttgca acagtggacg ctgacaaaga caaatagcca accattcctc caagtaatcc
    20821 actgaataca ttgccaatac ctgctatacg aagttccttg tttaaatcta attgcttatt
    20881 aactatcaat tcataactgc ttgcgtttaa taacaaggca ataaaactta ccagtataac
    20941 taaccctatg taattcaata gggtcattga gaatggataa tgaatcattt gccatatttc
    21001 aggtttcatg agcgaatcaa tatggctttg attgaaaggt cccaagacgt acccttcggt
    21061 tggatcgacc aaatactcca aattaaaaat ataaaaaaga gcatagaata gaattacaag
    21121 agaaataaac atcaattgaa gcgcgtatcg ataacctaat ttttctttaa aaatataaac
    21181 aactattccc gcaataaaac ctggcgccca caataatatt tcctctttat gaaaaaagac
    21241 aagccaattc tgatgcagcg ctttaccagt taataatgca aatgttcctg ataaaactag
    21301 ccagcctgtc gctgccataa aaccacaaat cacaggatat ggcagataac ggataatatt
    21361 tccaaaccta aattttccaa cgaagtagaa tgataagccc gttaaacagg tagtgactcc
    21421 aagcataaag agaatcgtaa taaagatggc ttcattcgta gaacttgatg gcatcgactt
    21481 aattatcact aaacccatgg ctgaatacaa aattgccgct tcatcttgca actgagcgat
    21541 tgcataagga ggggaagaca tgaacaaata gctggcgctt ataataatta ctgaaattgc
    21601 caatatatta atgacaagtg gaatataaat ctctaatgca ccttgataaa taagcgaaga
    21661 aaatgcaaca atactaatca tattgtcaat acttatgacc aagcctgcta aaatagccaa
    21721 aatcaattga cttcgagtca tcctacctct cactcattca tttcaaaacc aaaggattat
    21781 tttagtaaat aacagaaaaa tccagttttt cacatttctg atcgaattaa aatagcagcc
    21841 tccattcatt aaagattctg cataattttc tcttagcatg gatattgctt tacaagctat
    21901 tgttcatgaa actaaggata ttaataatta ttcgaataac cagataagca tttaaaattc
    21961 tttaatcaaa atggagatag tgctcaggtt aacaacatta caccgactct tggcaaaaat
    22021 atttaaactt aaccttatat attttgcacc gctatattct tatagctatt tattcttgta
    22081 attgcgcata attatcaatt aaggacgttt tgtaaaagcg ttcttgcttt aaggttatca
    22141 agagtcaaca aactatataa aaagttctgg aaatctactc cctctttccc ctaaactgtc
    22201 cttggttttt ttagacttaa ggactatata tgaaaactaa atcatggatt gccagtttgt
    22261 tttattcctc attatgtttc tcctcttctt acgcaattaa tctccatcaa atcacaataa
    22321 ctgatccaat aatgggcagt cgtaccatta cctatgaaca agttggagaa tttgcagtag
    22381 tggaaggtga tattttgatt ggtaaagcag agaatcttaa aaaccaaggt gctgttatta
    22441 caccaaaagt aggaggttca cgctggccaa atggtgttat cccttatgaa attgctgaaa
    22501 atttaccttt tataaacaag cttgccattt tacaagcaat agatcattgg cagaaaaaca
    22561 cgaatttaga attcgttgaa ttaacttcta aaaatcgcta tgactatcgt gattacattt
    22621 cttttatccc ggcagaaggc acaacctgct cttcctacgt tggacgccag ggagggaagc
    22681 aagagatcaa tttggcgcca agatgtacta ccatgaatac ggtacatgaa ataggacatg
    22741 ccttgggcat gtggcatgag caatcccgat cagacagaaa tcaatatatt cgtatcgtct
    22801 gggaaaatat tgatgaagaa cataaattta attttgacca acatttaact gatgggaaag
    22861 atttcggtga atacgactat ggatccatta tgcattatgg tcgatttgct ttttccaaga
    22921 atggtcagcc aactatcata cctctagttg atggagttga aatagggcag cgaactcatt
    22981 taagtgaaaa agatatcgct gccgtcaaag caatgtaccc tgaaacctaa ttgggagtgg
    23041 ctaaggccat atatccatcc ttttcctctg aaggagcggc taaagccaac tgtttggccg
    23101 ctttcgcttc agagtgttgg tcataagcat ggaaaatttc ataaccaatg tacatagctg
    23161 ttaaaacaat tgcagcaggc cagcaaattg caaatgttgt aattaaaact ataggcatga
    23221 ccgtattttt taccatggta aaaataaaat cattccgagc tgcttcatat tctttaagag
    23281 caactggaag gtttttacct gtcaattgag cctcttccaa atacaaactt ttttcctcgt
    23341 atttcgaata agatcccgta gacagataca ttgcaacagc aagagtacag gcaaaaaaac
    23401 aaccaacgac taatataggc gggctaacca gcatcgatgc agtaaatccc atcatcaata
    23461 aggcggcagc agcggcaaca aagtaaaaag tagcctcctg ggttttataa ttaatttcaa
    23521 gctctctaag ttgcttattg agcatctcta tatgcaataa tcgctgttca ggagtcattt
    23581 ttttgaattt ttccggatta ttatagtctt ctatttcctg caaatattga gctttcttaa
    23641 tcaaatattc ttgtttggct aagttgcact tatataaagc catgcatacg tcgaacccga
    23701 gaaaaacaga agtgatcatc cctgctgcag gagcggaaat attaaaaagg taattatagt
    23761 tggtaagcag gttgattgta gcccaaacaa agtcattggc aaaattacag tgtcgtttat
    23821 aaaactcatg tttaaatctg tcccatgccg tagtcacttc gggaacatgc cctgtttgat
    23881 gagtgattat ctcttctact tcagttgagt tcaaacgaag ggatgatgtg tccttattca
    23941 ttttcttcag taaagcatct ttcagtttgt tattttcttt aatgcttaat ttttctgctt
    24001 taccacttct aggaatgtaa tagagtgata cttcttgatt gtcttcatga acaagaatat
    24061 aactttttct atatgcttcg agactggctg ggcctggcaa ataatccatt tgatggattt
    24121 cacaaccatt ttctgcgcct ttttccaatt cagtagggaa aaaggtatgt cttaatagca
    24181 taccaccatc aattattaag cgcatcaaaa agaaaccaac actaaagtaa gttataaccc
    24241 cggtaggtgc ttgaagagtt gaaataatct tatcgacatc agtatgagtt cctaaaatag
    24301 catccaactt ctcaataatt tttaggtctc gagctacgct caatccatta gttaaagtca
    24361 aacgacaaaa cgtccaatag atacgacaca agttcgagta ggcaacataa tctctgattt
    24421 tggaagcgtg caatggagag ctgattaaat tgcgaaaacc tccaagaaag gaattgtaca
    24481 aggattgaat aaaaccagat tcttcctctt tgacattgac cttatttaaa tggtctttta
    24541 ttttttgttt tattttagca tattcagctt gtttactgtt ttgtgaataa gctttgtgga
    24601 aagcctccag caaggacgca caatagtagc aatacagcca gaaaatttct ttattttcat
    24661 cgtcttgctg ctgtaacaca gtaaacattt gagtgaattc agcttctaaa tcctgtttgt
    24721 gatgtagtaa aaaagtgtaa tctatagtgt ttactcgatc atcaattgtt acaatttttg
    24781 tgtccagttt actatccttc ggaatttggg cgaaaaattg ttgtttatcc ctacgaaatg
    24841 cagcactttg ttttgagtag accatattaa gaactcacaa aaattacaca ggaagtttta
    24901 caataatacg ttaccatttg atatgtattt tgacaagctt ttttgtatta atattttaca
    24961 cattgatttt tgcgattcag actgattttt gcataaaaat gaatcaagat aaaagctggt
    25021 tcatttgaaa tgtgctgaaa ctaaaagaat aaatttattg acccaataaa gcacatcacc
    25081 acaaaatatt taataataat tttgctgtat tcaaaagaag aaaaattgaa aattcacatg
    25141 atgactgcct tgcatcatcg ttattttcca atacaaaaac tggaaaaaat ttttcctaat
    25201 aaatcgtctg aagtgaactc acctgtaatt tcacataaag tctgatgagc taaccgtaaa
    25261 tcttctgcca acaattctcc agctttatga tttgttagct gcgattgccc agttagtaac
    25321 aaagcttttg cttcatccaa cgcttgtaga tgtcgtctgc gagctaaaaa ttggccttct
    25381 gttggttgat atccaaccac ttgtttaatc acctttttta gctcatccag tccctcacca
    25441 gtttttgcag acaaataaac tgtatgctca tcacattttg ctgtaagttt agtagtatca
    25501 attttattgt ataccgtgat tatgggtatt ttattgggta atgttaatct caattcatta
    25561 agcagagaat tttgttgatc aggattgtta atatctacta caagaagcac acaatcagct
    25621 cgcttcaact cttgccaagc acgttttatt ccctctttct ccaccagatc atcgctgtct
    25681 cttaatcctg ccgtatcgat aatgtgtaga gggatatcat ccaataggat atgttctcgc
    25741 ataatatctc gcgtagtacc ggcaatttca gtaacaatgg caacatcccg ccctgccagg
    25801 ttgttaatta aggttgattt tcctgcatta ggcctgccag caataaccaa ggataaccct
    25861 tcacgcaaca aaactccttg attcgcttgt gaacgtatat cttctaatcg tccaataatt
    25921 ttttgtagta attggctgac atttccatca ttaagaaaat caatttcctc ttcaggaaaa
    25981 tcgatagcag cttcaacata catgcgtaag taaattagtt cttcattcaa ttgatttatt
    26041 tttttagaaa aatcaccttg cagtgatttt aaagccatac gagccgcagt atctgaactg
    26101 gcttgaatta aatcagctat ggcttctgct tgaattaaat cgattttatc attaagaaat
    26161 gctctttcag aaaactcacc cggcctggct agccttgcgc ctgctgctat ggattcctta
    26221 attaataaat ccaaaactac aggtgagcca tgcgcttgaa tttcaataac atcctcacct
    26281 gtaaaagaat gaggtccttt aaaataaaga accaatcctt gatcaaggac ttcattattt
    26341 cctttatata aagagcaaaa agtggctaaa cgtggttgca atgctttgtt cccatttaga
    26401 caaagggcta tagcataagc atttggacct gatattctga cgattcctac cccgcctcgt
    26461 cctggcgggg tagcgatagc aacaatggta tctattgaca tttattttgc tgtggcaaca
    26521 acctttttag ctggcttttc atctgagtat tttctggtga tgtaccattg ctgtaatatt
    26581 gataaagtat tattcacaat ccagtacaaa accagaccag aaggaaaatt ccagaacagt
    26641 ccggtaaata atatgggtaa aaacatcatt accttagctt gcattggatc agctggtgct
    26701 ggattgagct tttgctgaat caacatggtt gctcccatga tcaatggaag aacatggtaa
    26761 ggatctgctg aagccaaatc atttatccaa aatataaatg gagcttgtct taactcaaca
    26821 ctttccagta aaacccaata gagcgctata aaaactggaa tttgaattaa tataggtaaa
    26881 cagccgccta aaggatttac tttttcttgc ttataaagtt ccatggtagc ttggcttatt
    26941 ttagctttat catcaccata tcgctcacgc aaagcttgca acttaggctg caacttccgc
    27001 atactagcca tggatttata gcttgtagct gataatcgat aaaatgccaa tttaattagg
    27061 acagtcacaa gaacaattga ccagccccaa ttcccaacaa cagtataaat tgctttcatt
    27121 aaagaaaata ataacgaaga caagaaccac aaaatgccat agtccaccgt taaatccaga
    27181 gatggggata tgccttttaa aacactggta atttctggtc ctatgtataa tttggatcct
    27241 acaattttat cttcttttgg tttgaccgta ataggctggc ttactgctcc aatagtataa
    27301 tctttatccg tagctaatgt ataaaacttg ttttcactat ccgcattggg aacccaagca
    27361 ctcaaaaaat agtgttgttg catagcaatc caaccacctt ttgcatccac atccaggtta
    27421 gatttgctca tgtcactgaa actaactttt tggtaacgat tttttccagg atttgaaaag
    27481 gaagcacctg tatacgagcc aatatgaaaa atactcgatt tgtcttcttt gggagaactg
    27541 cgcaataact gggtattaaa ataccctttc cataacgaat taccggtatt ggctatcttg
    27601 taattgactt cgattaaata acttcccttt gtaaaaacaa attctttttt cacatccaaa
    27661 ccgtcttcgc ttttgccatt tagagtgact atcagctggt tttgatcagg tttcaattcg
    27721 taatattctt tttctgatgt aaaatcaaaa tccaatgatt gtggtgtctg gccatctaaa
    27781 acaaaaaggc tactgttcgc tacatatctc tggctggcct gattttgcaa gagagggaat
    27841 ggtttatttt tatcttcaac acttagtgga taatcaagca ataaaccttt aacaatatct
    27901 ccatttttta aatcaatgtc gacatccaaa acatctgttt taacctgaat caattgttta
    27961 ccgctgcttg ctttttcctc tgctttcaaa gttacgggtt gctctgcatt agaaggaatt
    28021 atttgtggta acaaatgccc atctgatgtt aattggcttg ctgtcttctc ttcaactggt
    28081 tgtttggctg gataatcaat ttgccaagcg ttccaaagtg ataaaccaat aagcgcaagc
    28141 gccatatata aaactatacg tcgtatatcc attaaatatt ctcaattctt atcgttagga
    28201 aataaaggat catagccacc ttttgaccat ggatggcaac gagacaatct gttaagtgcc
    28261 atcaataaac ctttaattac gccataatgt ttgatcgcac tgtcagcata ctctgaacaa
    28321 ctcggataat agcgacaaca tggtgttatc aaagggctaa taaaatactg atacattttt
    28381 attggtaagc aaactatttg ccgtagcatg aggctaattt ttcccatgtt ttatttaatt
    28441 tagtatttat acctaaattg gttttctttg ctagacctgg tcttgccaaa atgattatat
    28501 ctacagcagg caagttagta tgacgaaaac tttctctaag tagccgttta atgcgatttc
    28561 tgtcatgagc cttagctatc attttttttg acaatgctaa accaaggcgt gcatagccta
    28621 acttattttc tctgaaaagg aaaataaagt catctgtagt tatttttttc gcgctttcaa
    28681 acacgaaatc aaaatcattt ttttttaata gccgttgcgc ttttttaaat gcaaacactt
    28741 aggcagataa acgcttacga cctttagcac gacgtctttt tataactaat ctaccagctc
    28801 gagtagacat tcttaaacga aaaccatgat cgcgtttacg ttttaaatta ctaggttgaa
    28861 aagtgcgttt catgataacc ctgcttctaa atttatgagg ttggcaatga tagataactt
    28921 tttttcaact gtcaatttga ataactgatt attttttgtg taatttttta tttacaggtc
    28981 gtaataatat taactttata tatttttaaa atttattttt attatataca aactgcttat
    29041 tgtgggtaat ttaaaaaaat actttgtttt taatagctta cattgattca aacactgttt
    29101 gtaactcctt tgttttattg tgatttattt gttgtctgtt tatctctcta atattgcaca
    29161 tacgttattt tatccttttt cacgtatttg aactgtactt atccatagaa aaataaattc
    29221 aaaataattt tattttgttc atattgtatt taaagttatc tttttatact ggtgttttat
    29281 tagtaaataa ttatagctaa tcgatttttg agtatatttc tatttattta aaaggtttga
    29341 tcttatttaa tttatctaaa ttatttgtat gtatattaaa atgtagaccc tattatggct
    29401 ttgtggataa ctttgccttt tctttaaacc cgggtataat ttattgatta ctcagataac
    29461 atatagattg aaatcttttt agattttaaa aaaaattacc aattgataaa ttcttgttat
    29521 taatctttat taattgtctt gctgatcggg tactttcaat ggcaatattt aatactgtgg
    29581 atagatagtt ttaagtattc ttttaccaca cttgtttaaa ttaggagtta ttttgtgtca
    29641 actactgctt ggcaaaaatg tttaggtttg ttgcaagatg aattttctgc tcagcaattt
    29701 aatacttggt tgcgcccttt acaagcttac atggacgagc agcgtttaat cttattagcg
    29761 cctaatcgtt tcgttgttga ttgggttaga aaacattttt tttctcgtat tgaagagttg
    29821 atcaaacagt tctccggcga tgacatcaaa gctatttcta ttgaagtagg tagtaagcct
    29881 gttgaagctg ttgatacgcc tgctgaaaca attgttactt catctagtac agcacctcta
    29941 aaatccgctc ctaaaaaagc agttgactat aaaagcagtc acttaaataa aaaatttgtt
    30001 tttgacagct ttgtcgaagg aaattcaaat caactggcga gagctgcgtc catgcaggta
    30061 gctgagcgcc ctggggatgc ttataatccc ttatttattt atgggggggt aggcttaggt
    30121 aaaacacatc tcatgcatgc cattggtaat agtattttaa agaataatcc tgaggctaag
    30181 gtattgtatt tgcattcaga acgttttgtt gcagatatgg ttaaagcatt gcaaacaaat
    30241 tctattaatg aatttaaacg tttttatcgt tctttaaatg ctttacttat tgatgatatt
    30301 cagttttttg cggggaaaga caggtcacaa gaggaatttt ttcatacttt taacgcttta
    30361 ctggaagggc aacagcaaat cattttgacc agtgatcgat atcctaagga aatagaaggg
    30421 atggaagagc gtttaaaatc tcgatttggt tggggattga ctgtagcagt ggagcctcca
    30481 gagttagaaa cgcgcgtggc aatattaatt agtaaagctg agcaatctaa tattgaattg
    30541 ccttatgaag tagccttttt tatagccaaa cgtatacgtt cgaatgtcag agaactcgaa
    30601 ggggccttgc gtcgagttat agccaatgct cactttactg gtaagccgat aactattgaa
    30661 tttgtacatg aagcattgcg tgatcttctt gctttacaag ataagttagt tactatagaa
    30721 aatatccaaa aaacggtagc tgaatattat aaagtaaagg tcgctgattt actttcaaaa
    30781 cgcagaagcc ggtctattgc aagaccacgg caaatggcaa tggctttgag taaagagttg
    30841 accaatcata gtttacctga aatcggtgat catttcggtg gtaaggatca tacgacagtc
    30901 atacatgctt gtcggaaggt taaagaatta atccaggatg atagcgattt tgctgaagat
    30961 tataaaaatt taatgcgaat attatcttct tgatacggag tctatcttgt ttgagctaac
    31021 cattaaaaaa gaagatctgc ttactccatt gcttactgtt tccggagccg tagataagaa
    31081 gcagtctttg gctattttgt cccatttttt attaaaactg tcggatggac ttttgtatat
    31141 tacggctaca gatttggaaa ttgaaatgtc cgcacaggtg gcatgtgatt ccaagaatac
    31201 tagtggttct attacagtac cagccaagaa attcattgat attatcaggt cattagatga
    31261 aaacgcttcg ccatctatcg tttttgataa agctttagtg acgataaaac aaaaccgaag
    31321 tacatttaaa ctggcaactt tgcctgctga gaattatcct agtagtgaag atgaatgtaa
    31381 tgatgtcgag ttaactatcc cgcgtttggt tttgttgcag ttattgcaat ctactcattt
    31441 tgctatgtct cagcaggatg ttcgtatatt tttgaatgga ttattgctgg agtttgacag
    31501 taatattatt tcagcggttg ctactgatgg gcacagaatg gctatatcgc gatattcctg
    31561 taataatatt gctcaaacaa aattgcttat acccaaaaaa ggggtgcaag aattattaag
    31621 attattgaat tgtattaatg acgaacaagt tttgttggca gcgggaaaat ctcatattaa
    31681 gttgattacc aatcaatttg tatttttgtc taaattaatt gaagcacgct ttccacctta
    31741 cactaaagct ataccaagag agcaggataa atgtattgtt attgattgcg cttcgctcaa
    31801 gcgtgcatta tctcgtattg tcattttagc tcatgaaaag tcaaaggctg tcttgttgca
    31861 tttacaacaa ggtcagttaa ctttaattgc caataataat gagcaagaag aggcaattga
    31921 aacattgagt gccgaaacac aaggtgatga actcaaaatc ggacttaatg caacctattt
    31981 gctggatgtt ttaactcatt tttctgaagg tcaaatcagg ttatcaatgt ctaatactga
    32041 aagcagtatt ttaattgagt cattagtcaa cgataattat caatacatta ttatgcctat
    32101 gaaaatatga ttttatctga agttagaatt cataattttc ggaatatcgc ttcgactagt
    32161 ttaatcctaa atcccaattt taattgtatt acaggaccta atggcggtgg aaaaacctcg
    32221 ttactagagg ctctttatat gttgagctgc gcccattctt ttcggtctcg tgagataact
    32281 ccaattattt cgtatggtca aaaccaactt aatgtttttg cccatgctta cgacgaaagc
    32341 actattagtg ttcagaaatc tataacagat ggcacacaaa ttaaattgaa taaccagttt
    32401 tgctgtacta caagtcaact agcctatgcg cttccttgcc aggttattta ctcagatatt
    32461 tttcaaatta ttgatgccgg cccctcggta aggagaagct tgcttgattg ggggttgttt
    32521 cacgtgaaac atgattattt gaaaatttgg aaggattaca agcgcatact tagccaacga
    32581 aatgccttat taaaatccag agctacttat gatcatttta ttccttggga ccaacagcta
    32641 agtcaattgg caaatcaact ggataaagct agaaatgact attttttgca atggcaacct
    32701 aaattctacc aagttttaag cgatctaacc aatatatctt gcacagttga gtattacaaa
    32761 gggtgggata gaaaaaatgc aggacagaac atggaggaac tgcttcagaa atcatttgat
    32821 agtgatagaa aaaaactata tacgcaatat ggaccccatc aagctgattt gataattagc
    32881 actgaacaat acagagtaaa gcatacactc tctcgtggac aacaaaaaat aattctaatc
    32941 gcgcttaaac tggctcaagg tcagctatta gataaagatt gcttatatct tattgatgat
    33001 ttggctgctg agttagatga ctatcaccag cgaaatttaa ttaagtatct gacacagcaa
    33061 aaagggcagt ttgttataac gaatttaatt aacaacaata attacgatat cctgccaatt
    33121 gactcaggtt tgtttgaagt aaactgtggc gcaattaaaa atttataata tagaatgttt
    33181 cacgtggaac acaaattaat ggaattaaaa gttctcatta ttaaacaata tcaccttaat
    33241 gcaaatgcaa tataaaattc taaaggtaaa catatgatat tctacaaatg gctcatcaaa
    33301 tgtcatggta tgaaaatcaa ataaacaaaa tgatattcaa aaaaaatcaa cgtcaataat
    33361 tcaaaatgca caagcgtaaa gttctctact taagatataa actagataca aaagatgata
    33421 taatagactc attgttaata gcataatacg gcaagagggc agctgatgag cgtagacgcc
    33481 agttatgatt caaataatat taaagtccta aaaggtttag acgcagtaag aaaaagaccg
    33541 ggcatgtaca ttggggatac agatgatgga accggtttgc atcatatggt ttttgaggtt
    33601 gtagataatt caatagatga atccctggca ggttattgca aggaaatttt tgttaccatt
    33661 catagcgatg aatcaattac cgttaaggat gatggtcgtg gtattcctgt cgatattcac
    33721 aaggaagaag gaaaatcagc tgctgaagtc attatgacag tcctgcatgc cggaggtaaa
    33781 tttgatgata actcttacaa ggtatcaggc ggtctacatg gagtaggggt atctgtggtg
    33841 aatgcccttt cagaagagtt gcatttacta gtgcgtagac atggaaaagt gtatgagcaa
    33901 cactacaggc atggtgttcc tgaatctcct attgctgaaa ctggagaagc caactctact
    33961 ggcacacaaa tatggtttaa accaagcgct gaaacctttt ctaatattga gttccattac
    34021 gacattctag ctaaaagatt acgtgaattg tcatacctga attcaggcgt ttgtattcac
    34081 ttatttgatg aaagaacaca gaaaaaagac acgtttcact acgaaggcgg catcaaagcc
    34141 tttgttgagc acttaaataa aaataaaaca ccagtccacc ccgttgtttt ctcaatgaca
    34201 tcggaaaaag acggtatcgt cattgaactg gccatgcaat ggaatgatgg ctatcaagaa
    34261 accatatttt gctttactaa caacatcccg caaagggatg gtggaacaca tatggccggc
    34321 tttagagcag cattaactcg cactttaaat aattacatag aaaacgaagg gtatgccaaa
    34381 aaggcaaaag tatctccaac aggtgatgat gctcgcgaag gtttaacagc tgttctttca
    34441 gtaaaagtcc ctgatcccaa gttttcatcg caaacgaaag ataaactggt ttcatctgaa
    34501 gtaaaaccag cagtagaatc ctgtgttgct gaaaaattta acgattttct tttagaaaat
    34561 cccactgcag caaaatccat cgtagcaaaa attattgatg cagcaagagc tagagaggca
    34621 gcgcggcgtg ctcgtgaaat gactcgacgt aaaggggctt tggatattgc cggtttacca
    34681 ggtaaattag ctgattgcca agaaaaagac ccatcgcttt ccgagttgta tttagttgag
    34741 ggtgactctg ctggaggctc agcaaaacaa gcgcgagata gaaaattcca ggctatttta
    34801 cctcttaaag gcaaaattct taatgttgaa aaagcacggt ttgacaagat gctttcttct
    34861 caagaagtag ccactttgat tactgcatta ggctgtggaa ttggtcccga ggaatatgat
    34921 ccagataaag tacgttatca tagaatcatc attatgacag atgctgatgt ggatgggtcc
    34981 cacataagaa ctttacttct tacctttttc tatagacaaa ccagagagct attagagcga
    35041 ggatatattt atattgcaca accacctttg tataaagtaa agcgtggaaa gcaggagcaa
    35101 tatgttaaag atgatgaagc gttatttgat tatctaactc agtgcgcgtt ggatggggcg
    35161 acattttatc ccgccaaaga ggttccgccc atatctgcca aatcgctgga agaattggtt
    35221 caacatttca gacgtgttga gaagattata aagcggtaca agagaagata ccccagcgat
    35281 atactaaaaa gagtagctta tatgccaatg ctgcaccaag aagattttaa caatctgcca
    35341 aaaattgaaa actggtgcag acaattacac ctaagcttac aacaagtaga cagtaaaaca
    35401 gaacaataca aagtagatac ccaaaaactt acagaaggta atcaatacac cccaaaaatt
    35461 actgtgtacc agcatggctt ggaaaatgat attccgatgc aagttgaatt tttcctgtct
    35521 aaagattaca aagaaatggt tgatttagga cataaattgt cgaatttaat agaggaaggc
    35581 gcctatattc aacgtggaga taaatccttg cctgtggaaa attttgagca agtacttaat
    35641 tggctaatgg atgaagcaaa aaaaggacaa acaattcagc gctataaagg tcttggagaa
    35701 atgaaccctg agcagttatg ggaaacaacc atggatcccg aggttagacg aatgttgcaa
    35761 gtgagcattg aagatgccgt agctgccgat gaaatcttta ccaccttaat gggcgacaat
    35821 gtagaaccaa gaagagattt tattgaaacg aatgctttag acgctgaaaa cattgatatt
    35881 taaacaaata gtattgttga actggagtga tgtttgcctc gctcgtggca aacgcctctc
    35941 tagcttatag ctggtccact caatgactta acttattttc ctactatgat gatgtgttca
    36001 aaaaaagcct tttgattgat tgcttaaaag aaaaaaatca aagaggtata ctggtaaatt
    36061 ggagaataag tgctgcgtcc ttgcagcgaa gtacatcctt tgtactgatc ccttagttga
    36121 cttcctgtca gacatatcca tgtcatccct gtatgttttt agtataacca attggcagta
    36181 tatgtcatcg agtataatga gcagtaatat gtaagaaatt tctcaattaa gaagtggcat
    36241 tgtcttcgtc attactccac taatttatga gatttaggtt gttgttgtgt aatacaatgg
    36301 ataccaccac caccagaaaa tacatcgagt gcatcaattt gtgtaatttg atatcctgga
    36361 aaaatttgta cgaataactg ataggctaat ctgtcaaatg attcatatcc aaaagcaggc
    36421 atcacaatac cttgattagc catgtaaaaa ttgatgtacg agagagtgag cctttctccg
    36481 ttaaggtaag ttgcaggtgg ttgctcaaca gtatagacct caaatgttcg tcctctggca
    36541 tccttagatg atttgagaat ttcaaagttt tcctgcaagc gatggtagtt aggatcttcc
    36601 ttatccttgg taataaggca taacaccttt ccaggagcaa taaatgttgc tatttcgtca
    36661 acatgaccat ctgtttcatc acctatcaat cccatgttca gccaaataat acgctcagca
    36721 cccagataat taataaggta ctgttcgatt tcctgttgag ataaatgtgg atttcgattg
    36781 gaatttaaca aacactcttt gcttgtcaaa atagtcccct caccatctac atgaaaggaa
    36841 ccaccttcca tgaccaatgg cgcatgaaag tattgagctt cagtgtattt tattagatga
    36901 gaagctatta aattatccaa agaacagtca gcgtaatttc caccccaagc attatgtatc
    36961 cagtcaactc cggcaagctg cctttcatta ttaattaaaa aggtagcccc cgtatcgcgg
    37021 gtccacgaat cgttaattgg caaagagata atttcaatat tatggccttt gcataaattc
    37081 tctgcggaat cttcatctcc tgggtttact aataaagtaa cgggctcaaa ttgagctata
    37141 gcttttgcaa cacgtgcata ggccatttta gccttgtcta gccctatttt ggaccaggtt
    37201 tcatggtgac agggccatgc catccagcat cgttcatgag gataccattc agcaggcata
    37261 taaaaaccat attgtttggg tgctgtattc atattcactg tgctatattt ccatcaaggt
    37321 aagttaattg agaaaaaata cttcttaatt cgtttaagta agagagtgcc ttttccttag
    37381 gaagttcaga tttaattaat tgcttttcat aagattgaat taactttttg gtatcaaaat
    37441 gggctaaatt caacacgttt gttacggtgt cgccgctgac taaatcatcc agctcaaatt
    37501 gtccatcatc agaaagcttt acatctagag aattggtatc tccaaacaag ttatgcagat
    37561 tacccaatat ttcctgatag gcaccaacca ggaaaaatcc gatgttataa gggttattga
    37621 tatcgtatga agggagcatc aatgtggttg taacgcagga acttccaggg tattgcttaa
    37681 ttgtaccatc tgaatcgcag gttaaatcct gtaggatact atgcatacta agtgttctat
    37741 taagttgaga tataggggct accgggaaaa tctgaccaat agcccaagca tcaggaatag
    37801 attggaaaaa agagatatta caaaatatct tggcagccat gcattcattg attctcatta
    37861 gcaaagcttt ttcacttgga ttttcttcat ccagtttatc ttttatttcc atacaaatat
    37921 tagtataaaa agcttctacc ttggcctttt cctgcaggct aattacccca tgcttgaaaa
    37981 gggaatgcgc ttcattaagc gaatgagctg cgtaattata tatttcagtg ggcgagctat
    38041 ctgtaatggc ttgataagta tcgtaaatat ctcgaatgac atgcgaatca tcttccgata
    38101 tttcaggaag aacgggcgct ttattgataa tctctacatc cgtaatatta gaaattaata
    38161 cagcatgatg ggctgttaat gctcgtcctg attcggagat aagatcaggc tctgatatct
    38221 cagcttcttg acataagtgt tttaaagcta acatgatgtg cgtagcatac tcaacaagac
    38281 tgtagttcat tgagcatcct ttattggatc gagtcccctc ataatcgaca ctcagacctc
    38341 cacccacatc gatggtacct atcggagcat tgagttgacg tagctcaata tagtatctcg
    38401 ccacttcctg catgcaatgg cgtatgtcat ggatgttggc aatttgtgaa cctaaatggc
    38461 agtgcattag ctgcaaacaa tccagcatat cattggcttt taattgtaaa atcagttcaa
    38521 gtacctgttt ggcatttagt ccaaactttg atttaacccc gccggtattt tcccacttgc
    38581 cagcactctt gctgaccagc cgaattcgca cacctaatgt tggtttgatt tgaaggcgtg
    38641 cagactcctt taaaataatg tccaactcag attttttttc aataacaata aaaaccttat
    38701 gacccatttg ttgagcaata agcgctgtac gaatataact tgtgtcttta tagccgttgc
    38761 aaactatagt actctgatgg tcacccagca tacctataac cgccatcaat tcaggtttgc
    38821 tgcctgcctc caggcctatc tgattatcag gcgattttaa caattcccgt accacacttt
    38881 gttcctggtt tactttaatt ggataaacca gctgataatt accggcataa caagtgtctt
    38941 ctcgagcttg ggtgaacgcc tgatatattc tatggactct atcatgaaga atatcactac
    39001 aacggatcaa taaaggcaaa tgcaagccag cacgatcagc tgcatcaact atagtttgca
    39061 gttcaacacc cggtttcccg ggttttttac tgatttcaat atttcccttg gagttaattc
    39121 ggaagtatcc ctcgccccaa ttttctatgt tgtaaagatt gtcatctgat agagtattat
    39181 tgttcattct aacgttccaa agaggattaa cgggatttga ctaattcatg ataagtttca
    39241 ggctctcgtt gttggggaaa tggaaataat cgcccccaca attcacgttg gctaaaatcc
    39301 agttcagcaa ccaatacggc aggttcatta cgcggtgcct gaactaaaat ttcgcccata
    39361 ggggtactga taaagctgct tccgtaaaaa cttaaaccat cttcacaccc aatccggttg
    39421 accgcaataa tgaatgtgtt actcataatt ccttgtgcta ccataacttt ttgccacatg
    39481 ggttggctat caaatcctgg cgcagtcggt tcccctccaa tcgctgtggg atagaccaaa
    39541 atttcagctc cttttaatcc ataaatcctt gataattccg ggaaccattg gtcatagcat
    39601 gttggtaatc ccaagtagtg accagcaatg gtatggacag gataattgga gttgcccggc
    39661 ttgaagtaaa agttttcatg atatttttca ccactgggga tatgttgctt tctggtaaca
    39721 gcaatcaatt ctccttgggg attaaaagca actgcggtat tgtaaccagc tttttcaaaa
    39781 agcgatgcgg taatatgaat ttgattcgac tttgccattt ggctaacaaa ttgagcagtg
    39841 ggaccagtgg cgatgtcctc catataaggg gcgggatcga cgtcactacg tgtacagaaa
    39901 taagggctta aggtaagctc ttgtaaacaa acgagttttg ccccttgctg ggtagctgaa
    39961 aatattccag aagccagttt atcttgatgt tctttaggat tttcatacca ttgttcctga
    40021 accagtgcga ccgttagttt gttttctttc attagagttc ccaataaatg atgttaagta
    40081 atttttatct cgaattcgaa tggcttactc ttgcttaagt taaatcatca acccagatta
    40141 aaaatctggc tatgttatca aaaaaagctc tctttgtact agcattaatt tcaattcact
    40201 gctcattttg gctgttacta attactaatg aattttcaga atggcagcta ttctgaaagt
    40261 ggtgaaagct cgcgactatc gattagaagc gaatctttcc tttcatttag gacagcacat
    40321 ttcgtttttc cttgattttt tgtgaggtaa ggggcttcta ttgttttaaa gctaaaaaag
    40381 taaacttgtt caatagtttt tctgtccgaa tcatttatta agtttaaagc ggcgttcaat
    40441 tgttaattta tgtctttaac tcgagcagat tgcaataagt caatgaattt atgagaaata
    40501 ttaacatgaa ttattgctac ctgatgtgta aagaatgggt aaaaaagtgt aaagttgccc
    40561 ttaaccaagt aagggcaaaa gatatggctg ctttacttgc caaataaccc acaaaaacta
    40621 gtccagcaat tctgtttctc ttcttcttct tcagaatctg agacaatggg ttccatctga
    40681 gagtttttct tcttgtgttc ttctgtttgt tctttaaata aaacgttcct gttgcgtaaa
    40741 ctggttggtg attcttgctt tggtacaatt tcttcagtag atggcaagac aacgggagtg
    40801 ggctttgtca ggcaaggctc ttctttaggc accatggcac tggctattat tctgaaaggg
    40861 cttgattgct cgtgagctgc tccattgatc atttccagtt ttctactgat atctgagcgt
    40921 actttagcat ccagcttagc taatttttct ccgatagctt gttcatggcc ttttaataat
    40981 tccgggaata aaattgcaaa ctcaaggcat aaatcaacgc taaaatctcg gctattgaaa
    41041 aattgttctg gattatcacc taatagattg cgctctatat cccttcttaa tgcaagggca
    41101 gattggactt ttatcgcaag ttcactatct tcggcacaaa aacctttaat tttcattact
    41161 acatcagtgc gattttcagc aggagttctc agtgcaagtt ttccggcaat agaagattct
    41221 ttatcattaa ttacattgaa taaacttgca aattcgatgc aagaatcgat cttgaattca
    41281 cgacttgaga agaattgatc aggcctgtca cctagcaata gtcgctctat gtctctgcga
    41341 acggcaaaag cgtggccgac tttagcggca aacagagact gtgggaaaat attgttgata
    41401 ttttgagata ctcgtgaacg atcttgagca ggagttgttt cagccagtct caatgcaatt
    41461 gcgacttcat tgttatttaa tacatcaata gccaatttat taaaattatt aaatagctca
    41521 ccatcaaatt ccttctccaa tatcatggaa tgagggtttt tattgcgcgg atcgagcaag
    41581 gaaatgattc ttttttttac ctgataagcc tggaataaaa aggaatgaaa ggtatcgcta
    41641 tcatcttgtg gcattcttgc tgcttcaatg gcatgagaaa aatcttttaa ttcattaacg
    41701 gggcactcca aaacgaggcg gctggctaaa actaattttt cgtcattgct taatttgttt
    41761 agctcttcgc ggttttccgc cagtgcgtca ccattgccaa gtacttcgta ggggtgctct
    41821 aatcctttta attcatcgca agtaaacgtc attaaaatct ccttattaac tcatcagtag
    41881 cctgattcta gatgtgcaaa aatattttga ttgactatat taacgaattc aaaccacgct
    41941 gtcatgaaaa tatactaaag ttttattata gggtgtcgaa taaatagtta gatacccgtt
    42001 tttgccttta aaaattacaa taacaacaag gagtaggcaa tgtctaaaaa tcatttacta
    42061 caagaccctt tcctaaatga attgcgcaag gaaaaggtac ctgtttcagt gttcctggtc
    42121 aatggtatta aattgcatgg gataatcgat tcttttgatc aatatgtggt gatgcttaaa
    42181 aactccataa cccaaatggt ttataaacac gccatttcaa cagttgttcc ttctcgaatg
    42241 gtcaaaattc ctgctgagga aagctctggt gaggaagaag gaactgtggc agactaatca
    42301 atttcttgtt tggggattag ggtagtgttt gaacgtccac aaggcggtga gcgagctgtt
    42361 ttggtgcagt tagctctccc cggggttgat gctgataagg cattggctga gtttgaagag
    42421 ttagcgcttt cggcgaaggc tgaggtgttg gattgtgtat taggtgcccg agctactccc
    42481 gatgccaaat attatgtagg gaaaggtaag gcggaggaaa tagctcattt ggtaaacatg
    42541 ctcgatgcgg atttagtgtt agtcaatcat gagctatcgc cttctcaaga gcggaatctc
    42601 gagcgattat ttggatgtcg agttgtcgat agaagtggct tgattcttga tatttttgct
    42661 cagagagctc gtacttttga gggtaaactt caagttgagt tagcccaatt gcaacattta
    42721 tcaacaaggc ttattcgtgg atggacccat ttggaaaggc aaaaaggagg gattggttta
    42781 agaggcccag gagaaactca attggaaacg gaccggcgtt tgttaagaga gcgcattcgt
    42841 tatatcaata agcgattgga aaaagtacgt tgcagccgcg atcaaaacag gcaggccagg
    42901 cggaaggcgt ccatgcccac tgtttctcta gtgggttaca ccaatgccgg aaaatccact
    42961 ttatttaatg ttttaacagg ggaacacact tatgtggctg atcaattgtt tgcaaccttg
    43021 gatccgacga tgcgtaaatt ggaattgcca gggtcctcag cagcaatttt agcagataca
    43081 gtaggattta ttcgagatct tccccatcac ttggtagaag cattccgcgc tactttagag
    43141 gaaacccagc aagctgatct attactgcat gtcattgata tttctgaccc gaattggcga
    43201 gagacggttt ttgaagtaca aaaggtgtta gatgaattaa aagttaataa tatcccggta
    43261 attcaggtat ttaataaaat tgatttgcag gaaggttggc aaccaaaaat tgattatact
    43321 gaggattctt gcaaggtgtg gttatcagca acaaccggcg ctggtctgga tcttctaaaa
    43381 gaggcaatag caactcaatt gcatggtatg attttaattg aggatattgt aattaagcct
    43441 gaccaggcaa aattacgagc ccaattatac caattaggtg ctgtacttaa agagtctgta
    43501 gatgaaaacg gcgattggtt aatgaaaatt agaataacgg ctgatcaaaa acgacgattg
    43561 ctttcatgaa taatcaagtc aggctgcggg gcgatagaat cgattccaga cttgataaat
    43621 tcacaagaaa atctttaact tattaaccat aaatataatt tttatttatc ttcttaggag
    43681 gtcctgttct ctgcaatgac tttttctaat gtttttgccg tttgtccccc gtataatttt
    43741 ttaattaatt gcccttttgg gttaaataca aaagttacag gcactcctgt gatgtctcct
    43801 aattgtaaat cctgagccgg atcctttaac aaattagggt agttaatatt aaatttttta
    43861 attagtaatt tttgctctgg taagggaaga gcatcgtaat tgactgcgta tagggctact
    43921 gcctcatttt tatggttttt gtaaaagcga ttaaattcag ggatttcatc gacacaggtt
    43981 tcacaccagc ttgcccaata gttgataaaa acccattttc ctttaagtga tgaaaaagga
    44041 atattgtgac caaaagtgtc tttcaaaagc gcttctgcat gagtagagaa gggagtaatt
    44101 gctaaaacca gcaggtatat gaaagtaggt attaagcgct tcatgatcac ctcttgaata
    44161 gtcaaaattg tttacggtta ttattgttaa ccaaattgga cataagaaaa tgcatgtatt
    44221 tgcttatgtc caacttacta actttaagac agctcaaatg ggttactaaa agttaaatag
    44281 taacaatagg ccactagttt atcaggaaac aatcagactc ccaaattcaa ccggacctct
    44341 tcttcctctt gaatggccga atgggtttgt tttgctgcca aaatactgtg tttctttaac
    44401 ccatccaaaa tatccagaga gtctttatta ttacgccagc tcgcttttgt tcgccaatcg
    44461 aatacagttg gttcgctttc cggaaggcat gatggtgtgg ggatgggagc tcgttgtgga
    44521 tttctgcgtt tttcatctgc gataatttcg gcgagctttt catcagaaac aggcataacc
    44581 gagccgtcat aaagataagg aaagagacgt aatttggtaa ctaatttaaa ggcagcccat
    44641 ttactggtta gctctttata cccatgtttt tcagggtttt ctctgagtgc ctcatattca
    44701 gaaggagaaa ttatgtgaat ttgactgaga ttgttgcggt aatatacttt tcctttactt
    44761 aaaaccagca atggttgatg ttgtcttgag ttaaaatcaa cgtcgggcat atgcaccaaa
    44821 ccaccgaatt ttttttgctc cggtattaca cctcctgata atacttgatt gatgtccttt
    44881 agtgatgcgg tcataccgtt taggcaagct aaacggttaa attgattaag gcaggtatca
    44941 aatttcccat ccccacgtgc tcgagacgga gcctctgttg tattaggggt tggatcatag
    45001 ggtctcactg tataaactcc aacacgattt acttcatcat gaagattatt agccaggata
    45061 ttgccccata ctccgaaacg ctcttttaat acgacacgta aatcgccaat catggacata
    45121 tgtcctgtag caaaatgatc agaatagtag tgaaaagtaa acagctctat agaataagct
    45181 tgggcgtgat agcgattcgc caaatcaagc agggtattgc ttgaaagagt ttcattctta
    45241 ttttgtaatg agatttttaa agtactcaag tcagggttat ctgattgata gtttctgtcg
    45301 gtagccaatt gttttaattc gtaggataaa cgtgcatatc tcaatgcgat agcgtgaccc
    45361 aatatataaa ctcgaataga ccatggcgta aaatgagttt ggttccgaac aagcatttcc
    45421 ccatagtctt tgacgcgaaa ataatacatc agttgttgag catagaaatt caacgtaggc
    45481 gaaaagggca catagttagc attattaatg gaataaatgc gatcaatttc ttttcgagta
    45541 acatcaggag cagccaggtt attgtatgct gtaattaaag cattctcttc tttaggatct
    45601 ataggtttgc gaatgaggtg ttttcccaat tctgccgggg agttgaatag ctcgcatttg
    45661 ggcagatcca aatccatagt ccaattagcc tgtgtaaagt agtcacccgc catggcaata
    45721 atttcgccag cggacatctc cagctccatt ggctcaggca ggttgttttc accgaatcgt
    45781 tgaagtcgca gatggagcac gccaaactca tctattcgta aatatggatt gccttcgatt
    45841 ttatccatga gcaaaccatt gcccagctct gtatgttccg ttgtgttcat aaattctgct
    45901 cctatcctta gagtctattt tcatcatatc cttgataact gaacgtgcca atagtttaaa
    45961 taatagtaaa tcaattagga aaccatacta ttttgaacta tcttaatata agacagtaat
    46021 taattggtaa ttaagatgaa agcaatggaa gtagagcagt taatccaggg aacattaata
    46081 cgagatggtg gaggagttaa attacgtcgc tatataggag ctgataaaac caatcacttt
    46141 gaacctatac tgctttttga ctattttgac agttcagatc cattggatta tctcgccggg
    46201 tttccgcctc atccgcatcg cggatttgaa acaattacct atctgatgga gggaagcatt
    46261 acccatgaag acaataaagg taacaaagga gtaataaacg caggtggtgt gcagtggatg
    46321 actgcaggaa aagggattat tcattcggaa atgccttcgt cttcaacgga ccggttgcat
    46381 ggtttgcagt tgtggttaaa tttgcctgct gcagaaaaat ggcgagaacc tcgttatcag
    46441 gaaaaatcat cggatcaact acctattgaa gtcatggaat caggtgcaac catcaaagta
    46501 atagcaggaa gaattgatca aggattgagt tcacccattg ttgaaattgc gacccgacct
    46561 ttgtttctag acatcactct ccctgccaat gcaactattc aacaaacgat tcctgatgat
    46621 tatcaagctg tcctttttgt cattgaaggt accgtgaata caggaggaca gcaaattacc
    46681 gcagggactt tagccagcct gacaaacggt gaaagaatac aaatagaagg caaagacaaa
    46741 gaaaatagaa atcattgttt actgatagct gcggctaaac ttcatgaacc tattgttcgc
    46801 tatggtcctt ttgtaatgaa tactcaagaa gaaattatgc aggcaataca tgattttcgt
    46861 acaggacatt tttagttgct tattgtgttg attttagccg gataattaaa actattcgga
    46921 atgatcttca tatattatct tggttgctca aaggattatg agtacaatgt gaatgattaa
    46981 acacttatga tttctgcgaa acttatttaa cgtgagttat ggatacttta tcttgaatcg
    47041 ttcgggctga ggaggcattt atgccgtctc
//
LOCUS       DACTJE010000004        45190 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.004, whole genome shotgun sequence.
ACCESSION   DACTJE010000004 DACTJE010000000
VERSION     DACTJE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 45190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45190
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.004"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            26..976
                     /locus_tag="I9760_000158"
     CDS             26..976
                     /locus_tag="I9760_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HAT5041960.1"
                     /translation="MKLKEIRTIIYNYGEEEILKHILYESTEPILIKIPDFTSQFSLD
                     YFEQLTQAKTTYCTFENKRCVDFQAGNFAEVIQQIKSNKPIRIFGQILSRERSKEIEK
                     HVPLWQKIPFRPRYFRETKKVAYFFGGKGAVSEMHFDREHCCNLHLCLSGKKQVLLFT
                     RKQSDNLYKLPFIGDSLIDFTQPLDALIQQYPRLNQAEGYQVLLEKGDMLFMPRNCWH
                     YTEYLDASSAATYVFYPKKFFQYYGYFTGNFFLGYKEESGFKIYRWPFFEKFSRYYAL
                     STGVSKIVFKIIEKILFLFMLPIISLFNIISHKFNPRRVF"
     gene            1248..4076
                     /locus_tag="I9760_000159"
     CDS             1248..4076
                     /locus_tag="I9760_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAT5041961.1"
                     /translation="MSEILEPVKDIPLTNQLELTANVPGMLKTIKRNGKVVIYDDTKI
                     KIAITKAFIADEGGTAPTSDRIHQQIEELTRQITQVFKRRLPSGGAIHIEEIQDQVEL
                     ALMRSGHYKVARSYVLYREERRKARESESKQKQTKDSKHLLITMPNGEVQPLDMDRVN
                     TIVKESCRDLSNVKAEPVIKDALRNLYNQAKYEDVHKALIMSARALVEKEPNYTYVSA
                     RLLLDSLRSEALTKLNIKSEATFDEMAQIYPVYFKSYIEQGIAQGMLDPKMADFDLDK
                     LSQALLPERDMKFTYLSLQTLYDRYFIHERGVRYELPQAFFMRVAMGLALREQHKEEK
                     AIEFYQLLSSFDYMSSTPTLFNSGTVRPQLSSCYLTTVPDHLDGIYSAIKDNALLSKY
                     AGGLGNDWTPVRAMGAHIKGTNGKSQGVVPFLNVADATAVAVNQGGKRKGAVCAYLEC
                     WHRDVEEFLELRKNTGDDRRRTHDMNTALWVPDLFMKRVREDGNWTLFSPDEVPDLHD
                     QYGKAFEALYVEYEEKARQGIIRNTKTISAVKLWRRMLSMLFETGHPWLTFKDPCNLR
                     SPQQHAGVIHSSNLCTEITLNTSEEEIAVCNLGSINLPAHIKNGKLDEQKLKRTISTA
                     VRMLDNVIDINYYSVPQARNSNLQHRPIGLGIMGFQDALYELKMDYASKEAIEFADSS
                     MELISYYAIEASCDLAKERGSYSTYEGSLWSKGILPIDSINLLQQSRNKYLEQDRSQK
                     LDWEPLRVKVRTQGMRNSNVMAIAPTATISNICGVSQSIEPTYQNLYVKSNLSGEFTV
                     VNPYLVADLKALNLWDEVMVNDLKYFNGSVQPISRIPDELKRRYATSFEIDPMWLVEA
                     ASRRQKWIDQAQSLNIYMAQPSGKKLDQLYMHAWIRGLKTTYYLRSLGASNAEKSTVT
                     DGALNAVKLIDTEPKACSITDPDCEACQ"
     gene            4090..5193
                     /locus_tag="I9760_000160"
     CDS             4090..5193
                     /locus_tag="I9760_000160"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAT5041962.1"
                     /translation="MNYNDTQEIIVTPLMGATGLETLEMGAARIHVDDKQIINCRADL
                     NQLVPFKYKWAWDKYLTACANHWMPNEISMSADVALWKDPYGLTEDERLIIKRNLGFF
                     STADSLVANNLVLAVYRHITNPECRQYLLRQAFEEALHTHAYQYIIESLGLDEAEVFN
                     MYREIPSVSRKAVWALPFTQSLGDETFHTGTPENDQRLLRDLIAFYVVFEGIFFYVGF
                     TQILSMGRRNKMVGTSEQFQYILRDESMHMNFGIDVINQIKIENPHLWTPEFKNEMIQ
                     LIREGVELEYQYARDTMPHGILGMNAEMFEEYLHFIANRRLNQIGLPDQYPGAENPFP
                     WMSEMLDLKKEKNFFETRVIEYQAGGTLSWDDE"
     gene            5508..5993
                     /locus_tag="I9760_000161"
     CDS             5508..5993
                     /locus_tag="I9760_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1776 family Dot/Icm T4SS effector"
                     /protein_id="HAT5041963.1"
                     /translation="MQSKIHKKEDNTNYSFLLKCMAAATAALATAAIVTAVVIKATAT
                     AAVGFGVATTGAALSPLLFVGGALLAAVGICCLPLLLCCGSSRSVYTVSNSPAYRPWS
                     SAWYTGGYNPTFHSHPVYNNGGTYHTHHSGTVHTHSNPVHTHGNNVHVHTTPSNVHGH
                     R"
     gene            complement(6080..7570)
                     /gene="lysS"
                     /locus_tag="I9760_000162"
     CDS             complement(6080..7570)
                     /gene="lysS"
                     /locus_tag="I9760_000162"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAT5041964.1"
                     /translation="MSEEHVHLDESEVYHIRKQKLAELRTGGFNFPNTFRREHLADAL
                     LKQYSETEKQTLEQKHVKVSVAGRIVLRRIMGKASFFHIQDVSGRIQVYLRSNDLPDI
                     YEQFKHWDLGDIVGVQGELFKTNTGELTINAEHVELLTKSLRPLPDKFHGLADQELKY
                     RKRYVDLIANEDSRKTFLIRSHLIKAFREFMDDNHFLEVETPMMHPIPGGALARPFVT
                     HHNTLDMTMYLRIAPELYLKRLVVGGFERVYEINRNFRNEGISTRHNPEFTMLEFYQA
                     YADYNDLMNFTEQLFHYLCDKVLATRQIEYQGQVIDFNKPFERLSVKEAILKYHPDIK
                     AQQLETVEGCRTLLNDLGLPYKETDGLGKLQIILFEETVEHQLFQPTFITEYPTEISP
                     LARRSDTNPEVTDRFEFFIAGREIANGFSELNDAEDQAERFRKQVEEKDAGDLEAMHF
                     DSDYIEALEYGLPPTAGEGIGIDRLVMLFTNSQSIRDVILFPHLRQ"
     gene            complement(7567..8674)
                     /gene="prfB"
                     /locus_tag="I9760_000163"
     CDS             complement(join(7567..8598,8600..8674))
                     /gene="prfB"
                     /locus_tag="I9760_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031433237.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAT5041965.1"
                     /translation="MLEVNQINLSLIDLNERIESLRGYLDFEGKSERLEEVIRELESS
                     NVWDNPEQAQALGRERTQLETIVHSLSQLSQSISDQRELFELAREEDDEQTINDIGEE
                     LKSIEQQVASLEFRRMFSGKMDHSNAYLDIQAGSGGTEAQDWAEMLLRMYLRWGEHHG
                     FTTELIECSPGEVAGIKSATIHFTGEYAFGWLRTETGVHRLVRKSPFDSGNRRHTSFA
                     AVFVSPEIDDDIEIEINPADLRIDTYRASGAGGQHVNRTDSAVRITHIPSGIVVQCQN
                     DRSQHKNKDQAMKQLRAKLYELEMQKKNAEQQALEASKSDIGWGSQIRSYVLDQSRIK
                     DLRTGVETSNTQAVLDGSLDQFIEASLKAGVGQA"
     gene            complement(8734..9135)
                     /locus_tag="I9760_000164"
     CDS             complement(8734..9135)
                     /locus_tag="I9760_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HAT5041966.1"
                     /translation="MTHIFLYLHAIIFLLLGNYLLNQRKNINKLQNKIDSLEKLATQV
                     VKDHPMLINADLLFAKQLNEINQQLISIETQLQELENIRHNDGSYQHALRILEMGGTR
                     EEIISSCHLSNAEAELLMNLQAYRTALKHQN"
     gene            complement(9132..9953)
                     /gene="motD"
                     /locus_tag="I9760_000165"
     CDS             complement(9132..9953)
                     /gene="motD"
                     /locus_tag="I9760_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HAT5041967.1"
                     /translation="MGNTSKMKSKQKKTEEHQDSHRWVVSYADFITLLFAFFVVMYAI
                     SSVNVSKYKSLSEGMKSAFNQKDQNKATQSTDNLKDGPEEKKTKGTFKDGLDELNKSL
                     SELEDGDFKLNRQEGWVEIDIKAGSLFDSGSADIKPEALIKLMKLAEKIKNLPFTVVV
                     EGYTDNVPIETPQYPSNWELSAARAATVGRILNGFGIANHRILVTGYGDQYPITDNYT
                     EAGRSQNRRVNILITKNRKVDRILNPDLSRVHFSVISNTNSVEFNKKDTKIKDKK"
     gene            complement(9910..10683)
                     /locus_tag="I9760_000166"
     CDS             complement(9910..10683)
                     /locus_tag="I9760_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HAT5041968.1"
                     /translation="MDTLTLIGLLTGFLAIIVGQIFEGGDLNSLLNFPALLIVMGGTL
                     GAVMVQTPLTTFKRAFRILPWVVKPPHHNFEECRTTLIELSRKARQLGLLSLEDRLDV
                     ETNPLIYKGLELLVTGVDKLTLRQVLEAEIDRTEHQDLRAAHVFESMGGYSPTIGILG
                     AVLGLIQVMRNLADPSQLGLGIAVAFVATIYGVGFANLFFLPVANKLKSCISHKVHFD
                     EMVVEGLVAMASGESPNMLQLKLNNYGQHKQDEIKTKEN"
     gene            complement(10693..11409)
                     /locus_tag="I9760_000167"
     CDS             complement(10693..11409)
                     /locus_tag="I9760_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAT5041969.1"
                     /translation="MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLD
                     DLIQSGMLGLLEAARHYDSSKGASFETYAGIRIRGHMLDEVRRNDWVPRSVYRNSRMI
                     SDAVRILEHKLGREAKDNEIAEELGVSLDDYHDMLQDSISSHLYGFDDLGVTDDVLFD
                     HEGNGSTEPHRNVMRSDMMNRLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSE
                     SRISQILSQATHRIRSRLPE"
     gene            complement(11506..12375)
                     /locus_tag="I9760_000168"
     CDS             complement(11506..12375)
                     /locus_tag="I9760_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HAT5041970.1"
                     /translation="MSKLSKGIKDQASGLRNLSRNKPVKVIAVSAGKGGVGKSNISVN
                     LAIALSQLNYSVMLLDADLGLANVDIMLGLHTKYNLSHVLQGICHLSDIFLHGPAGVR
                     VIPAASGTEFMTQLSPAEHAGIIDSFNELTDDLDYMIIDTAAGISETVLSFTRSSQEL
                     IVVVCDEPTSLTDAYALIKVMSKRYEWTHFHILANMVRNVKDGRDLFNKLFRVSGQFL
                     DVQLDYLGAIPFDENIHKSVKKQNPILIAYPDSPAAHALRELAESVSNWSFKPSLGGN
                     TSFFLERLVAGQF"
     gene            complement(12362..13501)
                     /gene="flhF"
                     /locus_tag="I9760_000169"
     CDS             complement(12362..13501)
                     /gene="flhF"
                     /locus_tag="I9760_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HAT5041971.1"
                     /translation="MKLKRFVAADTRSAMQQIKAAFGPDAVILSSHNIKDGVEIVAAI
                     DFDETVISTQAAVAIAEPPSRTEKYSAQTPLDEMRQEILTLRGMLEAQLRGNVGIHEP
                     LHTVMLQKLLYLGVSPNTATIWARSVKPDLNQQRAWQFILSQVTEQLPIRDSSRIEEG
                     GIYAFVGPTGVGKTTTLAKIAARFALRFGADKLGLVTMDTYRIAAQEQLILYGKILGV
                     KVCIAQDEVSLARVLRQLNDKKLILIDTAGMNPSDERVTKQMTVLSNYLQSIEKVLVL
                     PATSHYQVLIDAIKRYQANHVDQCIITKLDESLAVGGAISAVIESNLALGYLTDGQRV
                     PEDIKIATRHQLVELFAQQELQLFQEERDISKIHPNVRRENYVEA"
     gene            complement(13542..15620)
                     /gene="flhA"
                     /locus_tag="I9760_000170"
     CDS             complement(13542..15620)
                     /gene="flhA"
                     /locus_tag="I9760_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAT5041972.1"
                     /translation="MNNVMNYIEYWMRQGFGTPLILIVMISMMMLPLPPFLLDIFFTF
                     NIALSLVVLLAVIYSERPLDFAVFPTVILVATLLRLALNVASTRVVLLNGHNGTDAAG
                     QVIKSFGEVVIGGNYTVGIVVFIILVVINFVVVTKGAGRVSEVSARFTLDALPGKQMA
                     IDADLNAGLINQEQAIKRRTEVAQEADFYGSMDGASKFVRGDAIAGILVLIINIVGGL
                     IIGIFQYNMNFNDAVHNYILLTVGDGLVAQVPSLLLSTAAAIIVTRVSSAQNMGQAVI
                     SQVFAKPRSLVIAAAIIGLIGIIPGMPHIAFILLSAGLAGIAYLFINQEKVKREQQDK
                     EQNEFSQTSDSKLSELSWDDVNPVDVIGLEVGYRLIPMVDNAQGGILLSRIKGVRKKI
                     SQELGFLIPSVHVRDNLDLKPNHYRLSLAGVIMGESMIHPDKWLAINPGQVFGNLEGI
                     PTRDPAFGMDAVWINENQKEKAHTLGYTVVDASTVVATHLSMILEQNSQQLLGYEETQ
                     QLLDKLAISSPKLVKELVPDGLTLGMVSKVLQGLLSEHIPLTDIRTIVETLAEYASKS
                     KDTEYLISQVRIALSRLITQKISGLNEELPIITLKPELEQLLHNTLQNSGHGGVSFEP
                     GMADKIQQALIQFAAQQQAKQESSVLVVQPSIRTVLARFVRTISNDFHVLSYQEIPDN
                     KQIRIIGTVG"
     gene            complement(15617..16765)
                     /gene="flhB"
                     /locus_tag="I9760_000171"
     CDS             complement(15617..16765)
                     /gene="flhB"
                     /locus_tag="I9760_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HAT5041973.1"
                     /translation="MADEEQSQEKTEQPSSKRLKESRKKGQVARSKDFNATVILLFTG
                     LGFFIFGKQLSLQIASIMQQAFDFDRDILVTGNNSLENLFQLTKSGLWSVVPLLLVIF
                     ILSLLAPLLMGGWVFSGQSIQPKFSRLNVLKGLKRMISLKGFVEMLKAFLKFVLVASA
                     SILVLRSQVPLLLELGKAPLEIAITSGVMILLKSFVLISASLILIAAIDVPFQLYEHS
                     KSIKMTKQELKDEYKETEGKPEVKSAIRRAQQETARRRMMSEVPKADVILTNPTHYAV
                     ALSYQKKGKKAPVVIAKGKNLVAFQISKVAKEHKIPIISVPALARAIYFSTKLNAEIP
                     RGLYVAVAQVLAYIFQLRDRQRYDYKPEILQNVPIPPELAREAEEEIE"
     gene            complement(16765..17535)
                     /gene="fliR"
                     /locus_tag="I9760_000172"
     CDS             complement(16765..17535)
                     /gene="fliR"
                     /locus_tag="I9760_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HAT5041974.1"
                     /translation="MNLDYQTMIRLMSQFIWPMVRIGGLMLTVPVLSSALLPARIKIL
                     FVFTLSCVCFTYVPHNLSFENFNGLYLVYLVQELLLGLLMGFVLQIVFQVFILGGQII
                     SMQAGLGFAVMVDPTSNASVPLISQLYLMVTTLIFLALNGHLAILETLIESFRIMPIG
                     KSSLDPSVVWSTIMFSGWMFKEAVLISIPAILSLLIVSLSFGVMARVAPQLNLFSLGF
                     PITLLMGFVIIKIGLPTVGTEMVESIKHGIQFITGILR"
     gene            complement(17539..17808)
                     /gene="fliQ"
                     /locus_tag="I9760_000173"
     CDS             complement(17539..17808)
                     /gene="fliQ"
                     /locus_tag="I9760_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAT5041975.1"
                     /translation="MTPESVVSLFSDSVYIILLLLAVLILPGLIVGLIISMFQAATQI
                     NETSLSFIPKLFVTFLVLILAGPWLLKTLINYTDTLISNIPYLIG"
     gene            complement(17817..18566)
                     /gene="fliP"
                     /locus_tag="I9760_000174"
     CDS             complement(17817..18566)
                     /gene="fliP"
                     /locus_tag="I9760_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841869.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAT5041976.1"
                     /translation="MKIKSWLLILILFLLLPVAGHTDPLSFPVVTMQPGTDGSQSYSI
                     NLQILIFMTLLTVLPGLLMAMTSFTRIIIVLSILRQAIGMAQTPTNQILIGIALFMTY
                     FVMSPVFNKINETAIQPYMNERIDFSLALSNAQKPLRQFMLNQTRKNDLSLFEKFAHN
                     VPKSLDEIPMSVLIPAFITSELKTAFQIGFILFLPFLIIDLVVSSVLMAMGMMMLSPL
                     IISLPFKIMLFVMVDGWTLVLGSLASSFALA"
     gene            complement(18567..18977)
                     /gene="fliO"
                     /locus_tag="I9760_000175"
     CDS             complement(18567..18977)
                     /gene="fliO"
                     /locus_tag="I9760_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HAT5041977.1"
                     /translation="MKSKVLHLAFLSLSLISSVGWSSTLSPNTISYGELIRIISGLLF
                     VLFIIIFLSWIVKRMHRVNFTSSKGFQTVASMILGPKEKVVLLKIGDRYLLIGVGSYS
                     INLICDFGNQLPEGFDADQKSSFADVLKSAIGKS"
     gene            complement(18974..19303)
                     /gene="fliN"
                     /locus_tag="I9760_000176"
     CDS             complement(18974..19303)
                     /gene="fliN"
                     /locus_tag="I9760_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAT5041978.1"
                     /translation="MTQVNETSTEQLNQGNPLNPDPGNEKLELILDIPVSVTVEIGRT
                     KMTIRNLLQLNQGGIVALDRLAGDPLDVLVNGTLVAHGEVVVVNDKFGVRLTDIVSKA
                     ERIKRLK"
     gene            complement(19313..20374)
                     /gene="fliM"
                     /locus_tag="I9760_000177"
     CDS             complement(19313..20374)
                     /gene="fliM"
                     /locus_tag="I9760_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAT5041979.1"
                     /translation="MTEKDVLSQEEIDALLDSVDESIDDETNDEELSSRDQPVRKKPE
                     DTTSQELNSNFDSDKKVAEEGVKTLNFTGQERIVKGQLPVLDKIYDRAVRLFAADIYH
                     LTARDFEIKQDPLLITKHKEFMRSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLVDY
                     YFGGNSQFGAQKDKTDFTATELRVMEVVTKKLVTNLIHACEPIIQLDVTKFNDETNPQ
                     LVNIAEPEEMLLVARFVLNFGKETGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNW
                     INSLKEELMDVELTVSASMAETVSTLGQVMAWKVGDFVPLEINEEVTLDIEGTPSFTA
                     TLGSANEKRALKVIKIIRY"
     gene            complement(20819..21058)
                     /locus_tag="I9760_000178"
     CDS             complement(20819..21058)
                     /locus_tag="I9760_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041980.1"
                     /translation="MNKIFNLASIACFLFFSLIAFAADIPGLDEGTDKLNMEECVGEN
                     YQQCLDDVCLTSDEIDCNSNCQSLAEDKCEQQAVD"
     gene            21177..22472
                     /locus_tag="I9760_000179"
     CDS             21177..22472
                     /locus_tag="I9760_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAT5041981.1"
                     /translation="MRSKRILLTNFTRAIYSESCCIRPDTEKELIHYIAQNQPKNMLT
                     RGAGLSYSDSCLNQDGLVIDTSRFNHFIDFDVETGIVVCQGGTPFKDLFLLHDEFIPP
                     VLPGTAFATVAGGIAHDVHGKNNHSAGSFGHHISWFDLLIGDQIMHCSREKNSDLFFA
                     TIAGLGLTGIITQVAIRLKKAPRHLAIKNKPFASIQELIAEMATYGLQQDYQVAWLDL
                     LYSEPRAILSTANYCESPNYPDKKLKIYSLPKLPFGLIKKWNMKVFNQYFYSRQKENE
                     MMSLTQYNNPLDKISHWNRLYGPRGLIQFQAVFDQDKAAQIINHILEIIRTHSATPTL
                     TVLKLFTQPGLGLLSFCRPGFTLAIDFINNPHAKKAISAMNQFIAENDGCVYLAKDLL
                     LNPEQYFTMYENQSKFAQILTKYRCPMRSDLATRLGIIK"
     gene            22469..23191
                     /locus_tag="I9760_000180"
     CDS             22469..23191
                     /locus_tag="I9760_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAT5041982.1"
                     /translation="MMQRKWVVLGATSVIAEQFAHFAAQAGNHLCLVARNSEQLALIS
                     KDIQLRYKIPCNAITINLSEPNEQLLTILETRERELDLFIAHCDFTDNNHLNPQSIKH
                     LIDTNISSTAILINTYLQSQQEKHRIVYLSSVASCRGRAKNSLYGASKAAIEIFLEGL
                     QQKATPHQHILIARLGYIDTKQTYGLPGVFYAAQPHDCAKACWEKINHNKRLFYYPSF
                     WKVVMAIITKLPFFIYKRMGKI"
     gene            23310..24218
                     /locus_tag="I9760_000181"
                     /pseudo
     CDS             23310..24218
                     /locus_tag="I9760_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636831.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(24228..24449)
                     /locus_tag="I9760_000182"
     CDS             complement(24228..24449)
                     /locus_tag="I9760_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041983.1"
                     /translation="MDNGGTLEGVSRLFSVGTTTIKNWKRLRAQTGKLEGSGRPCNPY
                     KIDADALKLYLKERPDAFLNGNPPIFNWQ"
     gene            24547..24711
                     /locus_tag="I9760_000183"
                     /pseudo
     CDS             24547..24711
                     /locus_tag="I9760_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611931.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            25201..26481
                     /gene="rvfA"
                     /locus_tag="I9760_000184"
     CDS             25201..26481
                     /gene="rvfA"
                     /locus_tag="I9760_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RvfA"
                     /protein_id="HAT5041984.1"
                     /translation="MFKFIYCINQEHIMSITIHTWKGGTKGLGFKTPVKSTLFGGNVG
                     HAALELTWPANKQGDELAKKYAQAHGVIISKRTEIVGETQEEGRIMPKERVTYFAYFS
                     WWPGEQNGHYINSFNDDKDAEWRHEPDKKMHADWKNQIYGPQGMSEENTTDVKGILIK
                     NKNIQKLKTIQHSTLTADIAQDKAYNELLQHKEKLDEEYKSLHAKAVAYQKEKENAIR
                     EEREINPNFELLDEDISRMGDLPREFSILEAQLNLCIADFNERHLSSGKKPDSSVILP
                     TSMDETSLNHTLDTESILREMITLANSEKAYNFTKFNCSTSVCHIVKAGVDEKLKENL
                     SDNGFNINRYVTSYITTPTNVNALGLKLQDALLKLELAEHTKEAEQVQKPVANTISNR
                     FNEFKSRLQAVVSERNAHKDDTEEVTINNHNSLR"
     gene            complement(26666..27862)
                     /gene="mavB"
                     /locus_tag="I9760_000185"
     CDS             complement(26666..27862)
                     /gene="mavB"
                     /locus_tag="I9760_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavB"
                     /protein_id="HAT5041985.1"
                     /translation="MPPPYSTHVIVNVFRCVAQLVSQNPELLNWPGVFRLAGSREESE
                     KLLEQVINDNFSVGNLACYILEDGQINNLHLNNVLGMLPLVLKESVLLKTEDPLLKLF
                     MTELKNLLSSEKNNVENTDAIVQLLSKFIDSLLLSKILDHQRVGEILYHYCYLMHTAG
                     VFYETNLMTWENLAIIMAPHFTNEFGLYPAEDLLGLIQFTNQLKPILECFIAHPDSGI
                     PFKERHADKLEHLAKTRHTIVEKLTHMGSESRRIVVAPMKSLMLQASMLRAQIDTVET
                     QSKDSSLKKKDKKELNRQLEKLTEELEKLNIEISGLTSKIKKMNHGHAKLKEEIRVIS
                     RSEDAVSTHAAQQSGVSSSSSNSVRIQLGIFGNNSSSSAYLIEEQEEGEVTENEYSGL
                     DSAKLT"
     gene            complement(28099..28296)
                     /locus_tag="I9760_000186"
     CDS             complement(28099..28296)
                     /locus_tag="I9760_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041986.1"
                     /translation="MNDLLNLIAVIVVFGVGLWLINVFIPMPGAIKSLLNVLVLIILI
                     IYILQFFGVIKTILPMIKILK"
     gene            complement(28387..30969)
                     /gene="alaS"
                     /locus_tag="I9760_000187"
     CDS             complement(28387..30969)
                     /gene="alaS"
                     /locus_tag="I9760_000187"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAT5041987.1"
                     /translation="MKSSEIRQAFLNYFVQRGHQIVASSSLVPSNDPTLLFTNAGMVQ
                     FKDLFLGLETRSYQRAATAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     REAIQYAWEFLTEVLHIPAERLWVTVYKEDLEAEDIWLKEMKVSPERFSRCGEKDNFW
                     SMGDTGPCGPCTEIFYDHGPEVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGHLHP
                     LPKPSVDTGMGLERLAAVIQGVHSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADH
                     IRSCSFLIVDGVLPSNEGRGYVLRRIIRRAVRHGNKLGLPSPFFSKLVQPLIDVMGDA
                     YPELINSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLQGQELSGEVAFKLYDTYG
                     FPIDLTADIIREQGLHIDMEAFNQLMQQQREQSQAASQFTTDYHAVSQLDHQSEFHGY
                     EKESMEAKIIGLLQEGNEVKSINKGAKGAVILDHTPFYAESGGQVGDKGLLIGKEFTF
                     QVDDTQKVGQAVVHYGKVIKGELTLDLLIHAQVDNIRRDAIRLNHTATHLLHAALKKI
                     VGQHVQQRGSLVDAERARFDFSHFEALTPQQIQQIEEVVNAQIRANNEVITQVMDIES
                     AKQSGAVALFGEKYSDAVRVLSMGDFSKELCGGTHARRTGDIGLFKIVAEYGIASGIR
                     RIEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEKVSQLLLDNKNQEKMIAKLLS
                     EKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMDQLKSRIDSAVIILFTIEQ
                     NKMNVIAGVSKNIIGKAPSAAQLVRHLCGKGGGRDDMAQGGGGVPEDLNSKIKEIKEM
                     IEKI"
     gene            complement(30987..31439)
                     /gene="recX"
                     /locus_tag="I9760_000188"
     CDS             complement(30987..31439)
                     /gene="recX"
                     /locus_tag="I9760_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAT5041988.1"
                     /translation="MTKAFDSALRLLTRREYSAMELCDKLKQKGFSTNDVQNALYECQ
                     RLGYQSDVRFVENYIRVRIHQGYGPLKIRQELKNKGIDPDLIQSVLHQEKDNWVNYAL
                     RAWEKKFKRQDDFSYSEIQKQQRFLLYRGFDRDVISKVFKEVKSSYLI"
     gene            complement(31432..32478)
                     /gene="recA"
                     /locus_tag="I9760_000189"
     CDS             complement(31432..32478)
                     /gene="recA"
                     /locus_tag="I9760_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAT5041989.1"
                     /translation="MEENKQKALSAALSQIERQFGKGSVMRMGDSTVSRDIEAISTGS
                     LGLDIALGIGGLPKGRIVEIYGPESSGKTTLTLQVIAECQKMGGTAAFIDAEHALDPS
                     YAQKLGVKVDELLVSQPDTGEQALEITDMLVRSAAVDVVIIDSVAALTPKAEIEGEMG
                     DSHVGLQARLMSQALRKLTANIKRSNTLVIFINQIRMKIGVMFGSPETTTGGNALKFY
                     ASVRLDIRRIGSIKKGEEILGSETRVKVVKNKVAPPFKMTEFDILYNEGISRESEIIN
                     LGVQLNLIEKSGAWYSYKQEKIGQGKENVRLYLKENPQVAAELEQQIRTELLEKKLSV
                     LASSSEDLFETIDD"
     gene            complement(32781..33716)
                     /locus_tag="I9760_000190"
     CDS             complement(32781..33716)
                     /locus_tag="I9760_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1803 family Dot/Icm T4SS effector"
                     /protein_id="HAT5041990.1"
                     /translation="MSQYTRLFEFLCDLIDIQIKKYVELAKLKVGEDAIINAALIYEE
                     VNELLGFAEKLLKEIETNSKSDSMDMTIFDRVFEQVKFYVEQEYLRSYAEWLLIENNS
                     KDTVYNRTKEQLLRLSEIKSFDDKGFSQPSTEIQQQCQHDSVQIVYSILNLAREVRKN
                     PDMGFDDSIPHNKLNVLRRNATTRYVEFGNEIDKLHQHSEEFLQQSSLGVFDAELAYE
                     SALSKAKDHDLKLNTLFGRYKENKETASVPQSLSLVNKVDEENIMTEKKPSTEVSDNK
                     TPFFSTSNLLFFGGIVLAAVIFVPRLLTHLKVNKL"
     gene            complement(34008..34502)
                     /locus_tag="I9760_000191"
     CDS             complement(34008..34502)
                     /locus_tag="I9760_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HAT5041991.1"
                     /translation="MGHFSILVEEIAAYLKKAKWQLVTAESCTGGLIAACLTEIPGSS
                     NWFERGFVTYSNLAKEEMLAVAHELIQTHGAVSEEVALAMAVGALQHSMGNIAVSVTG
                     IAGPEGGSIEKPVGTVCFAWAVHHQFQQTSRQQFTGSRQEIRLSACQYALNGVLSTLK
                     KVCG"
     gene            complement(34579..34773)
                     /locus_tag="I9760_000192"
     CDS             complement(34579..34773)
                     /locus_tag="I9760_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041992.1"
                     /translation="MNRLAMIAAASCLALFLTGCGEGSKPADTKTDQQQQQQPTDQQQ
                     QGGENTGNNQAQPSTEGQGQ"
     gene            35121..37661
                     /gene="mutS"
                     /locus_tag="I9760_000193"
     CDS             35121..37661
                     /gene="mutS"
                     /locus_tag="I9760_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAT5041993.1"
                     /translation="MASSHTPMMQQYLRIKTDYPDMLLFYRMGDFYELFFDDAKRASQ
                     LLDLTLTHRGQSADKPIPMAGVPYHAVENYLARLLKKGESVAICEQIGDPATSKGPVE
                     RQVTRIITPGTVTDEALLDARKDNILLAIHTQKQKIGIAWVDLGGGRFHLQELTEEHQ
                     LNAELVRLQPAELLCKESTPLPSFCSNFAVKFRPGWEFDASNAHKLLCEQFSVTDLSA
                     FGEQNYPTALIAAGALLAYLKTTQKQSLPHLTTLTLEQSEDYLQLDASTQKHLELFEN
                     IHGGGEHCLLSILDKTACAMGSRLLKRWLGKPLKQHAIIQTRQQAIKEIIFLQQDVSL
                     HQLIKQCADVERIVSRIALKSARPRDLVSLLQTLTLLPAIHDELQENKSLLINEIKKE
                     ISPLPLLQQLLETAIIDNPPMLIRDGGVIAPGFDEELDELRNLSSNAHETLVKLEQEE
                     KNRTGLSTLKLGYNSVQGFYIELSKAQAQNAPPHFHRKQTLKNVERYITPELKLFEDK
                     VLSAQSKALAREKWLYDNLLEEIQQYIPELSDLAKSLAQLDVLVTLAERAQSLNWNCP
                     NLVPESGIMIQAGRHPVIEPLLQERFIANDLELKPNQNMLLITGPNMGGKSTYMRQTA
                     LIVLLAHIGSFVPADKVTLGPLDRIFTRIGASDDLSSGRSTFMVEMTETAQILRQATS
                     QSLVLIDEIGRGTSTYDGMALAYASCAFLASTIKAYTLFSTHYLELTELPKEFSCIRN
                     VHLQASIKTGQIVFLYRVEEGCANRSYGLEVAELAGIPKEVLKLAHEHLNQIQDTQSI
                     LVQTQIIKPPTSPVLTELKKIDPDRLTAKEALDLIYKLKHLECAESIN"
     gene            37674..39362
                     /locus_tag="I9760_000194"
     CDS             37674..39362
                     /locus_tag="I9760_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HAT5041994.1"
                     /translation="MNRFAYLIILIILYFPLFAQDKKAGLLEIDGIKGKVLANVETRL
                     GELSQIKPLSQFTPTELQDQINKAIQPFGYFNAETSINNLNNKIIIKVQPGSQIRIAS
                     IKAMLTGEGAQNPLLRKTLKELPLHIGDPLFSEQYEKAKQNIINTAENMGYLHGVFKK
                     AEILIDEHKKSAQITLIFDTGPQYYFGQVQFDPTYISPQLLHRFVPFDPGQPYATDQV
                     LKLNDYLSNSGYFSSVLVKPQITDAQTVPVIVHLQPVPKYSYSFGLGYGTDTGVRGKA
                     ALHVIPVNRQGHKFNAVAQGSFRQNALQAQYVIPGKNPVTDQYALTGNFSNLNYNAGY
                     SNATLLSLSQLHNVNQFQRALSLNALYESFHYSLQPNTDQFLLYPKANITFSKTKNLL
                     FSPSGYNITFNALGANKAVLSHLNFGQLSLDAKAALTLDSLHLRLYGHTIQGITAIND
                     INELPLSLALLLGGTDNLKAYSFNSIGPGKIITYGGFEIQKEFKKNWYLVGFYDAGDV
                     YNPSVKNIQYDIGGGLMWVSPIGPIKVGLAQSVDNKMERIGHNPRLVISMGPDL"
     gene            39359..41899
                     /locus_tag="I9760_000195"
     CDS             39359..41899
                     /locus_tag="I9760_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF490 domain-containing protein"
                     /protein_id="HAT5041995.1"
                     /translation="MMILKFIKKGFYYCLITAICLVSLIIFLISTTPGLYAVIKLTHL
                     YLPGTLKIHHLKGQLLDQFSIGEMEYQHQDTKIKIFNLAVNWRFSSLLRKKLPIDSMS
                     AEKIEINQKTPILILKNIKLSGYLNQQLIKLNTLQFNYWNQLITSQLEVSNSFPHAFS
                     GKIRLNPHSTNKNIFSGGVDIGGNRNQTQWGGEFEGPGKITINGSLQQLKQIKQIIKW
                     RDLHWQDNKNHNLKSSQGRIEVSGTIPNLAIDLKSKLGIDQLKNWQMNASIKGKLPWQ
                     WSVNATFSQPLDFSSKLDGLYTKFALKMETQSDHHANFLITIAPGHYQMTENNLMPLI
                     QFTGGNIKGSLSPKQFSGQGVLFLDAYKKVNFNFKFPEFNVYQDIAQQTIDAKLSILF
                     NSLDFLQNVSPYFMNPKGQLLASIHAKGTLNKPRIESKLTLNKGSVNIPTLGLNITDM
                     GLAVFSKKDGWEATGSIHSSEKKLTLKGQGSFNKELGGTVSIEGSNFPVANTKEFQVN
                     ISPQLNVRFTPSLCQITGTIQIPNAHIKPQTFTNSLVATDDIVFTSKNTQPITPLFNT
                     NMSVRVEMGEEVELTFKGLHAYLAGIVTINQLSQGPITANGELAVKKGEYKAYGQDLS
                     IEQGQLIFTGGRIDNPEINLRASKNISNSFGSVSGSSELFNFNNYNNLNVGNNFNVGV
                     EVAGRLQSPKIQLFSNPPTLSQADILSFLVIGRPANQADKAKGQLLLTAISSMNLGTG
                     TNGTQLLEQLKQKLGFDFNVQTTTNFNQQTNKISESTGVVVGKSLSDRIYLSYNVGLS
                     QTDPNVLTLKYILNKFLSIQVSSSDSGNGVDVLYTKSSSSPKKTKPDK"
     gene            41933..42928
                     /gene="hemB"
                     /locus_tag="I9760_000196"
     CDS             41933..42928
                     /gene="hemB"
                     /locus_tag="I9760_000196"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAT5041996.1"
                     /translation="MNEYNLPLRLSRLRKNAMSRALVRETRLHAQQLIAPLFISEYLT
                     EPKEIGAMPGQFQLGLNCIPEEIEILSKSGIPAVILFGIPKHKDAYGSESFNSNGIIQ
                     QAIKKIKSVNSDMLVITDLCFCEYTDHGHCGVLNGTQINIAATLELLGKQAVSHAEAG
                     ADWVAPSGMTDGMVLAIRQALDHASFHEIPILSYSAKYCSCLYGPFREAAQGAPQFGD
                     RKAYQMDPANSEEAVRETRLDLEEGADMIMVKPAGFYLDIIYKLKQHFADVPLCAYQV
                     SGEYSMIKNAAKNNLINEEQAMTESLIAIKRAGADFIISYFAKGMAELLNSQNFI"
     gene            43065..43457
                     /locus_tag="I9760_000197"
     CDS             43065..43457
                     /locus_tag="I9760_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5041997.1"
                     /translation="MNLMLKGLSALAISLGAATTFAAPAYLTTHNRTGEESNAYIAGS
                     IPSLYPTAAYSTNQVYWNLVRLACYGHTTNGQCPALIKMATNTANPIDIGYVTMDLNT
                     GDITPKTLSAKGYSLRVIGPGEAEITKN"
     gene            complement(43755..45164)
                     /locus_tag="I9760_000198"
     CDS             complement(43755..45164)
                     /locus_tag="I9760_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAT5041998.1"
                     /translation="MSTAVLGVLATGTLNAGGFSLYTEGAGYTTGNFAAGVAAEAADA
                     STGWYNPAGLVLIRDQQAVFAGVGVFPTAKLSGVSTYSTINPPPFPSFVYSEVFNNIN
                     GAEDAFVPSFHYALPLGENATFGLSVVSPFGLATDWGPDSPVRYQATFTELITTNISP
                     EIGGRLTEHVALGAGLDLQYARVKYNRILGVPNILNVLPPFNPMLADSLTYNKGHSFG
                     VGFHVGVLGMFNDNHTRLGANYQSSMRHEFYGYSRLTGPLASPGNILAPFLFPAPGIF
                     QSDDLFSNSIDFPDVVTISAYHDVNDKLALLGSLVYTAWSSYKTVQLSNAAAGRPADN
                     GNVSQAKAISISPQNYNDTLRVAIGANYHVNDKLMLRVGGGYDPTPTNNIDRDVRLPD
                     TDRWALSAGAHYQATNNIGLDIGYTHLFTSSDPVINRTDAFTATTRYNVTASGTAGAN
                     LVGVQATWTIDKEPPAPTK"
BASE COUNT        14497 a         9245 c         7752 g        13696 t
ORIGIN      
        1 attactccat tcaacaggta atttaatgaa attaaaagaa atcagaacaa tcatctacaa
       61 ctatggcgag gaagaaatcc ttaagcatat cttgtatgaa tccacagagc ctatcttaat
      121 taaaatacct gattttacca gccaattttc actcgattat tttgaacaac tcactcaggc
      181 caaaactact tattgtacct ttgaaaacaa gcgatgtgtt gatttccaag cgggcaattt
      241 tgctgaggtc atccaacaaa taaaaagcaa caaacccatt cgtatttttg ggcaaatttt
      301 gtctagagaa cgctctaagg aaatagaaaa gcatgtgcca ctttggcaaa aaattccttt
      361 cagaccaagg tattttagag agaccaaaaa agtggcttat ttctttggag gtaaaggcgc
      421 tgtctcagaa atgcattttg accgagaaca ttgctgcaat ctccatttat gtttgagtgg
      481 gaaaaaacaa gtattgctgt ttaccagaaa acaaagtgac aacctttaca aactcccctt
      541 tatcggagat tctttaattg acttcactca acccctggat gctttaattc aacaataccc
      601 cagactgaat caggctgaag gatatcaggt tctacttgag aaaggcgata tgttattcat
      661 gcctagaaat tgttggcatt acacagagta tcttgatgcg tcatctgccg ctacttatgt
      721 tttttatcct aaaaaatttt tccaatatta tggatatttt accggcaatt tttttttagg
      781 gtataaagaa gaatccggtt ttaaaattta tagatggcct ttttttgaga aattcagccg
      841 ttactatgca ttatcaacag gagtctccaa aatcgtgttt aaaataatag aaaaaatttt
      901 atttctattc atgctgccta taattagcct atttaacatt atttctcata aatttaaccc
      961 ccgcagagta ttttaaccca caacttatcc acagaataat cccagattat tctattgacc
     1021 aaagcaaaaa aacacattat cttgttatta aaacccctaa aacaccctat atattgggtt
     1081 ttttgctata attatggtaa cagtaaacaa aagcttctct tgactgtcgt aaaaacagga
     1141 gcaaatcata gtttcaatga aactaggatc gccttttaaa caagttcgta aaacatgttg
     1201 gattcactga aacaatgaat tgctaaaata tatttcgtgg aggaaaaatg tccgaaattc
     1261 ttgagccggt aaaggatata ccgctaacaa atcaactgga attgactgct aatgtcccag
     1321 gcatgcttaa aacaataaaa cgaaatggca aggtagtcat ttatgatgat actaaaatca
     1381 aaattgctat taccaaagcc tttattgccg acgaaggtgg tacagcccca acttctgatc
     1441 gaattcacca gcaaattgaa gagctaacca gacaaatcac ccaggtattt aaacgtcgtt
     1501 tacctagtgg tggggctatt catattgaag agatccagga tcaagttgaa ctcgccctta
     1561 tgcgcagcgg acattataaa gtagctcgct cctatgtttt atatagagaa gaacgcagaa
     1621 aagcacgtga atcagaatcg aaacaaaaac aaacaaaaga cagtaagcat ttattaataa
     1681 ctatgcctaa tggagaagta caaccattag atatggacag ggtcaatact atcgtcaaag
     1741 aatcatgtcg tgacctcagc aacgtcaaag cagaacctgt tattaaagat gctttgcgca
     1801 atctttataa ccaagccaaa tatgaagacg tccataaagc tttgattatg tcagcgcgtg
     1861 cgttggttga aaaagaacct aattatactt atgtcagcgc gcgtttgttg ctagacagct
     1921 tacgttctga agcactgact aaactcaata tcaaatcaga agctacattt gatgaaatgg
     1981 ctcaaatcta tccagtttat ttcaaatctt acatagagca aggtattgcc caaggtatgc
     2041 ttgaccccaa gatggctgac ttcgacctgg acaagcttag ccaagctctt cttcccgaac
     2101 gagatatgaa gttcacctat ttaagtttac aaactttata tgatcgttat tttattcatg
     2161 agcgcggggt aagatatgag cttcctcaag cttttttcat gcgagtagcc atgggtcttg
     2221 ctcttcgtga acaacataag gaagagaaag ccatagagtt ttatcagttg ctttcttcgt
     2281 ttgattatat gtcttccact ccaaccttat ttaattccgg aacagtaagg ccgcagttat
     2341 ccagttgcta tttaacaaca gtacccgatc atttggatgg tatttatagc gctataaaag
     2401 acaatgccct cctctctaaa tatgctggag gactaggcaa tgactggact ccagtacgag
     2461 ctatgggtgc ccatatcaaa gggactaatg gtaaatctca aggcgtagtt cctttcctga
     2521 atgttgccga tgctactgct gtagcagtaa atcagggggg gaaacgcaag ggagctgtct
     2581 gcgcgtacct ggaatgctgg cacagagatg tagaggaatt tctggagtta agaaaaaata
     2641 ccggtgatga tcgccgccgt actcacgaca tgaatactgc cctttgggta cctgatttgt
     2701 ttatgaagcg tgttcgtgaa gatggtaatt ggactttatt ttcacctgat gaagtacccg
     2761 atttacatga tcaatatggt aaagcatttg aggctctcta tgttgaatat gaagaaaaag
     2821 ctcgacaagg tattattagg aatacaaaaa ccatttcagc tgtcaaacta tggcgtagaa
     2881 tgctttccat gctttttgaa actggtcatc catggctaac attcaaagat ccctgcaatt
     2941 tacgatcacc tcaacaacat gctggagtaa tacacagttc taatttatgt acagagatca
     3001 ctctaaatac ttctgaagaa gaaattgctg tgtgcaattt aggcagtatc aatcttccag
     3061 cgcatattaa aaacggtaaa ctggatgagc aaaaactcaa gcgcacaata tcaacagctg
     3121 tccgtatgtt ggataatgtc attgatatta attattattc tgtacctcaa gctcgtaact
     3181 ccaacttgca acatcggcca attggtctag gtatcatggg ctttcaggat gcgctatacg
     3241 aattaaaaat ggattatgca tccaaagaag ccattgaatt tgctgactct tccatggaac
     3301 taatcagtta ctatgctatc gaagcctctt gtgacctggc taaagagcgg ggcagttact
     3361 caacctatga ggggtctcta tggagcaaag gcatattacc tatcgattca attaacctct
     3421 tgcaacaatc ccgtaataaa tatctggagc aagacaggtc acaaaaactg gattgggaac
     3481 cattacgagt taaagttcga acacaaggca tgcgtaactc aaatgtaatg gctattgcac
     3541 ctacagccac aatatccaac atctgcggtg tatcacaatc tattgaacca acttatcaga
     3601 acctttacgt caaatccaac ttgtccggcg aatttaccgt ggtgaatcct tatttggtag
     3661 ccgatttaaa agctctgaat ctttgggatg aggtcatggt caatgatcta aaatatttta
     3721 atggcagtgt acaacctatc agtcgaattc cagatgagct gaaaagacgt tatgccactt
     3781 cttttgaaat tgaccccatg tggttggttg aagccgcgtc acgccgtcaa aaatggatag
     3841 atcaagccca gtccttgaac atctatatgg cgcaaccttc tggtaaaaaa ctggatcagc
     3901 tttatatgca tgcatggatt cgtggattaa aaaccactta ctacttacgt agtttaggtg
     3961 caagtaatgc tgaaaaatcc actgttaccg acggggcact taacgcagtc aaattaatag
     4021 ataccgaacc aaaggcttgt tctattactg atccagactg cgaggcttgc caataatcag
     4081 gagaaattta tgaactacaa tgatactcaa gaaattatcg taactcctct tatgggcgcg
     4141 acagggctcg aaactttaga aatgggggct gcacgcattc atgttgatga taagcaaatc
     4201 attaattgcc gtgctgactt aaatcaattg gtgcctttca aatacaaatg ggcttgggat
     4261 aaatacttga cagcctgtgc caatcattgg atgcccaatg aaatcagtat gagtgccgat
     4321 gtcgctttat ggaaagatcc ttatggtctt acagaagatg agcgtttaat cattaagcgt
     4381 aatttgggat ttttctcaac cgccgattca ctcgttgcga ataatctggt tcttgctgtt
     4441 tacagacata ttactaatcc tgagtgtcga caatatcttc taaggcaagc ttttgaagaa
     4501 gcgttacaca ctcatgctta tcaatacatt atagagagtt tggggctgga tgaagctgaa
     4561 gtatttaata tgtatagaga aataccttct gtatccagga aagcagtttg ggcattacca
     4621 tttactcaaa gtttaggtga tgaaaccttc catacaggca ctccagaaaa tgatcaacgc
     4681 ctgctacgcg atttaattgc attttatgtc gtgtttgagg gtattttctt ttatgtcgga
     4741 tttactcaaa ttctctctat gggacgtcgt aacaaaatgg taggaacatc agaacagttc
     4801 caatatatac tgcgcgatga gtccatgcat atgaatttcg gtattgatgt gattaatcaa
     4861 atcaaaatcg aaaatcctca cttatggaca cctgaattta agaatgaaat gatccaactc
     4921 atcagagaag gtgttgagct cgaataccaa tacgccagag acactatgcc tcatggcatt
     4981 ctcggaatga acgccgagat gtttgaagaa tatttgcact ttattgcaaa ccgtcgctta
     5041 aatcaaatag gtctgccgga tcaataccca ggtgctgaaa acccatttcc ctggatgagt
     5101 gaaatgctgg atctcaaaaa agagaaaaac ttttttgaaa ctcgtgttat tgaatatcag
     5161 gctggtggta ctttaagctg ggatgatgaa taaagtttaa tgatgttacc tctagcaaag
     5221 taagggagtt catttagaga aatcccttac tttgtgaaaa atgtcatacc cctgtcttta
     5281 acttaaccaa acttaataat attctacgta tttccattat ttttgaatta attttaaaca
     5341 aatcaacctc taaatccaaa tttatggtga cattttagtt tgatcaggta aaatcccaag
     5401 cgcatagaaa taatgctatg ccagcaaaat aataacaaca tcgctaaaag atataacaca
     5461 cccagaaaaa tgcattctat taactgaaaa tcaagatgag gtaagttatg cagtctaaaa
     5521 tacacaaaaa agaagacaat acaaattaca gctttctatt aaaatgtatg gctgctgcta
     5581 cagcggcact tgctacagca gcaatagtaa ccgctgtggt aatcaaagcc actgcgacag
     5641 cggcagtagg ttttggtgtt gcaacaacag gagccgctct atcgccatta ttatttgtag
     5701 gaggagcatt actggccgct gtgggtatct gctgccttcc attattgctc tgctgtggca
     5761 gcagccgtag tgtatatacc gtgtcaaact caccagccta caggccttgg agcagtgcat
     5821 ggtatacagg aggctataat ccaacttttc attcacatcc tgtttataac aatggcggta
     5881 cttaccacac tcatcactca ggaaccgtac atacccactc taatccagta catacacatg
     5941 gtaataatgt tcacgtccat actactccaa gtaatgttca tggacataga taattttatc
     6001 taaaccaagt gtattttatg tattgcttga catagcaatg gttctaaaca aaccattgct
     6061 atctaaaaat cgctgtactt tactgtctca aatgcggaaa tagaataacg tctctgattg
     6121 attgcgaatt agtaaataac atcaccaagc gatcgattcc aatcccttca ccagctgtag
     6181 gcggcaagcc atattccaat gcttcaatat aatcactatc aaaatgcatt gcttctaaat
     6241 cacccgcatc cttttcttca acttgctttc gaaagcgctc agcttgatcc tccgcatcgt
     6301 ttaattcaga aaatccattc gcaatctcac gaccagcaat aaaaaactca aatcgatcgg
     6361 tgacttccgg attggtatca gaacgtctcg ctaaaggaga tatttctgta gggtattccg
     6421 taataaaagt gggctgaaat aattggtgct ctactgtctc ctcaaaaaga ataatttgta
     6481 gtttacccag gccatcagtt tctttataag gcagacctaa atcattcaga agtgttctgc
     6541 atccctctac cgtttctaac tgctgcgcct taatatcagg atgatatttt aatatagctt
     6601 cttttacgct caaccgttca aaaggtttat tgaaatcaat gacctggcct tgatattcta
     6661 tttgccgagt agctaatact ttatcgcata gataatgaaa caattgttca gtaaaattca
     6721 tcaaatcatt gtaatcggca taagcctggt aaaattccag catcgtgaac tctggattat
     6781 gacgagtaga aattccttcg ttacggaaat ttctattaat ttcatatact ctctcaaaac
     6841 caccaacaac caagcgtttt aagtacaatt cgggggctat cctcaaatac atggtcatat
     6901 ccaatgtatt gtgatgagtt acgaaagggc gagccaaagc acctccagga attggatgca
     6961 tcattggtgt ttctacttcc agaaaatgat tatcatccat aaattctctg aaggctttga
     7021 ttaaatgtga ccgtattaga aaagtttttc gactgtcttc atttgcaatc aagtcaacat
     7081 aacgttttcg atatttcaat tcctgatcag ccaaaccatg gaatttatca ggaagtggcc
     7141 ttaaagattt ggttaacaac tcaacatgtt ctgcattaat ggtcagttca ccagtgtttg
     7201 ttttaaacaa ctcaccctgt acaccaacaa tatctcctaa atcccaatgt ttaaactgtt
     7261 cataaatgtc aggaagatca tttgaacgaa gataaacttg tatccgacct gatacatcct
     7321 gaatatgaaa aaaactggct ttacccataa ttcttctcag aacgattcgg cctgcaacag
     7381 atactttcac atgcttttgt tctaatgttt gtttctcagt ttcagagtat tgcttcagca
     7441 aagcatcggc taaatgttcg cggcgaaaag tgtttggaaa attaaaccca cctgtgcgca
     7501 attccgctaa tttttgcttg cgaatatgat aaacttcact ttcatctaaa tgtacatgtt
     7561 cttcactcat gcttgcccta ctcctgcctt taaactggct tcaataaatt gatctaatga
     7621 accatccaat actgcctgag tattactggt ttcaacaccg gtgcgcaaat cttttatacg
     7681 agattggtct aatacgtaag aacgaatttg tgatccccaa ccaatgtcag atttgctagc
     7741 ttccaatgct tgctgttccg catttttctt ttgcatttcc aactcataaa gctttgccct
     7801 caattgcttc attgcctggt ctttattttt atgctggctt cgatcattct ggcactgaac
     7861 gactatccca ctagggatat gtgtaattct tacggcagaa tcagtacggt taacatgctg
     7921 cccaccagcc ccggaagctc tgtaagtatc aatccgcaaa tcagctggat taatttcaat
     7981 ttcgatatca tcgtcaattt caggagagac gaaaacagct gcaaacgaag tgtgtctcct
     8041 gttgccagag tcaaatggcg actttctcac taaacgatgg acacctgttt cagtccttaa
     8101 ccaaccaaac gcatattcac ctgtaaaatg aatagtcgca cttttaattc ctgccacttc
     8161 accaggagag cattctatca actcggtggt aaatccgtga tgttctcccc aacgtaaata
     8221 cattcgtaat aacatttcag cccaatcttg cgcttcagtg ccgccagaac ctgcttgaat
     8281 atcaagataa gcattggagt ggtccatttt gccagaaaac attcttctaa attccaaact
     8341 ggcgacttgt tgctcaattg attttaattc ctcaccaatg tcgttgatgg tttgctcatc
     8401 atcttcttct cgagccaatt caaacagttc cctctgatca ctaattgatt ggcttaattg
     8461 gcttaatgag tggactatag tttccagctg agttctttca cgtcccaatg cctgagcctg
     8521 ttccggattg tcccacacat tggatgattc caattcgcga attacttctt ccaagcgctc
     8581 acttttacct tcgaagtcaa agataccccc taagcgattc tatgcgctca ttaagatcga
     8641 ttaaacttaa attaatttga ttcacttcta gcatcagtgt ctcttttaaa aataaatagg
     8701 agattttaca gcgaaagtaa gattctatct atactaattc tgatgtttta atgcggttct
     8761 atacgcctga agattcatta gtaactctgc ttcagcattt gataaatgac aactactgat
     8821 aatttcctca cgagtgccac ccatttccag gattctcaag gcatgttgat aactgccatc
     8881 gttatgtctt atgttttcca attcttgcaa ttgagtctca atactaatta attgctgatt
     8941 aatttcatta agttgtttcg caaataataa atccgcattg atcagcatag gatgatcttt
     9001 tactacttga gtagccaatt tttccaaaga atctattttg ttctgtaatt tattgatgtt
     9061 ttttcgttga tttaataaat aattacccaa gagtaaaaaa attatcgcat gcaaatacag
     9121 aaagatatgt gtcatttttt atccttaatt ttagtatcct ttttattaaa ctctactgaa
     9181 tttgtattac ttataacact aaaatggact ctacttaaat ctggattgag aattctgtcc
     9241 acttttctat ttttagtgat aagaatattc actcttcgat tctggcttct tccagcttca
     9301 gtgtaattgt ccgttattgg gtattgatca ccatatcctg taaccaaaat acgatgattg
     9361 gcaattccaa aaccatttaa aatgcgacca acagtagctg ctcttgcagc tgataattcc
     9421 caatttgaag ggtattgggg cgtttcaata ggcacattat cagtgtatcc ctcaacaacc
     9481 actgtaaaag gtagattctt aattttctcc gccagcttca ttagtttaat caatgcttct
     9541 ggtttaatat cagcactacc agaatcaaat aatgagcctg cttttatatc tatctcaacc
     9601 catccctctt gtctgtttaa tttgaaatca ccatcttcaa gttcagatag tgatttattc
     9661 agttcatcca aaccatcttt aaaagttccc ttggtcttct tttcttcagg accatcttta
     9721 aggttatctg tcgattgagt cgctttattt tgatcctttt gattaaatgc cgacttcatt
     9781 ccttcagaaa gagatttata ttttgataca tttactgaag aaatagcata catcacaaca
     9841 aaaaaagcaa ataataaggt aatgaaatca gcataagata cgacccatcg atgactatcc
     9901 tgatgttctt cagttttctt ttgttttgat ttcatcttgc ttgtgttgcc catagttgtt
     9961 taactttaac tgcagcatat tgggactctc accactcgcc atggcgacca ggccttcaac
    10021 gaccatttca tcaaaatgca ccttatgaga aatacaactt tttaatttat tagcaacagg
    10081 taaaaaaaac agattcgcga acccaacacc ataaatagtt gcaacaaaag caaccgcaat
    10141 gcctaaacct aattgactgg gatcagcgag attcctcatg acttgaatta aacccaacac
    10201 cgcacctaaa attcctatgg tggggctata gccgcccatg ctttcaaaga catgcgcagc
    10261 acgcaaatcc tgatgctccg ttcgatctat ttctgcttcc aaaacttgtc gtaaggtaag
    10321 cttgtccact cctgtaacca gtaattccaa ccccttataa attaaaggat tggtttcgac
    10381 atccaaacga tcttctaggg agagaaggcc aagttggcga gcttttctag ataactctat
    10441 gagagtcgtt ctgcattctt caaaattatg atgaggaggt ttcactaccc aaggcaatat
    10501 tctaaacgcc cttttaaaag tggttaaagg agtttgcacc ataacagccc ctaatgttcc
    10561 acccatgaca atcagtaagg ccggaaaatt aagtaaggaa tttaaatctc ccccctcaaa
    10621 aatttggcct acgataatgg cgagaaaacc tgtcaacaat cctattaaag tcaaagtatc
    10681 catacttatt ttttattccg gtaatcttga tctaatacgg tgcgttgctt gacttaaaat
    10741 ctgtgaaatt cgtgactcac taacctccag tatttccccg atttccttca aattcaaatc
    10801 ctgctcataa tacaaagaaa gcaccagcct ctctttacga gggagactat caatcacttg
    10861 ggttaatcga ttcatcatat cacttcgcat aacattcctg tgcggttcag ttgagccatt
    10921 cccttcatga tcaaataaga catcatcggt tactcccaag tcatcaaaac catataaatg
    10981 actgctaatg gagtcttgca acatatcatg ataatcatct aatgacacac ctagctcttc
    11041 agcaatttca ttatccttag cctcacgccc aagtttatgc tccaatatgc gcacagcatc
    11101 agaaatcatt ctggaattgc gataaacaga acgcggcacc caatcattac gtcttacttc
    11161 atcaagcata tgtcccctga tacgtattcc tgcataagtt tcaaaggatg cccccttgga
    11221 tgaatcataa tgtcttgctg cttcaagcag accgagcatc cctgattgta tcaagtcatc
    11281 cagttgtaca ctctgcggta atcgcccgag cagatgatgt gcaatgcgtt ttaccaatgt
    11341 ggcatgtgtt tttaccagag cttcctgggt ttgttgattt actttgctgt atgcagccaa
    11401 agcatccacg atactctcct tacaataaca gcagattgta gttattagta atttggtaaa
    11461 tctaaaacaa acagagtaca gctaatttta agtatagcga ataatttaaa attgtcccgc
    11521 aaccaaacgc tccaaaaaga aactggtgtt tccacctaaa gacggtttaa atgaccaatt
    11581 actcaccgat tcagctaatt ctctcaaggc atgagcagca ggtgagtcgg gataagcgat
    11641 taaaatagga ttttgttttt ttactgattt atggatgttt tcgtcaaaag gaatcgcgcc
    11701 gaggtaatct aactgcacat ccagaaactg tcctgagacc cgaaacagtt tattaaacag
    11761 gtcacgacca tccttaacat ttctaaccat attagccaga atgtggaaat gcgtccactc
    11821 ataacgtttg ctcatcactt taattagtgc ataagcatct gtcaaagaag taggttcatc
    11881 acagacaaca acaatcaatt cttgcgatga acgagtaaaa ctcaatacag tctcggaaat
    11941 ccctgctgcg gtatcaataa tcatatagtc caaatcatca gttagttcgt taaatgaatc
    12001 aatgattccc gcatgttccg caggactcaa ctgcgtcata aactctgttc cggaagcagc
    12061 aggaataacc ctgacgccag cgggaccatg taaaaaaata tcacttaaat gacatatgcc
    12121 ttgaagaaca tgagataaat tgtatttggt atgcaacccc agcataatat ccacatttgc
    12181 caaacctaaa tcggcatcca gaagcataac actataattt agttgagata acgctatggc
    12241 taaattgacc gagatattac ttttgcctac accgcctttg cccgctgaaa cagcaatcac
    12301 tttgacaggt ttgtttcggg acaaatttct aagaccagaa gcctgatctt ttataccttt
    12361 gctaagcttc gacataattc tctctcctaa cattcggatg tattttgctg atgtcccttt
    12421 cctcctgaaa taactgaagt tcttgttgcg caaataactc aacaagctgg tgacgcgtag
    12481 caattttgat atcctccggg actcgttgtc catcggttaa ataacccaag gctaaatttg
    12541 attcaataac agcacttatg gctccaccta ctgcaagaga ttcatccaat ttcgtaataa
    12601 tgcactgatc cacatgattt gcctggtaac gcttaatcgc atcgattaat acctggtaat
    12661 gactggtagc tggtaatacc aatacttttt ctatagattg cagataatta cttaaaacag
    12721 tcatttgttt ggttacacgt tcatcagaag gattcatacc ggctgtatca atcaaaatta
    12781 attttttgtc atttaattga cgcaatactc ttgccaaaga gacttcatct tgcgcaatac
    12841 agactttaac tcccaaaatc ttcccataga gaattaattg ttcctgagct gctattcggt
    12901 aggtgtccat agtgaccaaa cccaacttat cagcaccaaa tcgtaaagca aagcgtgctg
    12961 caatttttgc tagagttgtt gttttaccaa ctccagttgg accaacaaac gcgtagatac
    13021 caccttcttc tattctacta gaatcacgaa ttggcaattg ctctgtgact tgtgacaaaa
    13081 taaattgcca ggctctttgt tgattcaaat caggttttac tgagcgagcc caaattgtcg
    13141 ccgtattggg actaacacct aaatacaaga gtttttgcag cataactgta tgtaaaggct
    13201 catgaatacc aacatttcca cgcagctgtg cttcgagcat tcctctcagt gtcaaaattt
    13261 cttgccgcat ttcgtctaat ggcgtttgcg ctgaatattt ttctgttcgg cttgggggtt
    13321 ctgctatagc tacagcagct tgagttgata tcactgtttc gtcaaaatca atagcagcaa
    13381 ctatttcaac accatctttt atgttatggc ttgacaaaat aacggcatca gggccaaatg
    13441 cagcctttat ttgctgcatg gcactgcgag tatctgcagc gacaaagcgt tttaatttca
    13501 taatgcctcc tgccttcaac acacctatgc aaccccaaaa actaacctac agttccaatt
    13561 attcttattt gtttgttatc aggtatttcc tgataagaca aaacatgaaa gtcattggat
    13621 attgtacgca caaatcgtgc caaaaccgtt cgtatacttg gttgaactac caaaactgag
    13681 ctctcctgct ttgcctgttg ttgtgcagcg aactggatta gtgcttgttg gattttatct
    13741 gccattcctg gctcaaagct aactccgcca tgtccactgt tttgtagagt attgtgcaac
    13801 aattgttcca actctggttt taatgtaata atgggcaatt cctcgtttaa tccactgatt
    13861 ttctgagtta tcaatcgtga taaagcaatt ctcacttggc taatcaaata ttccgtatct
    13921 tttgattttg acgcatattc cgccaaagtt tcgactatag tgcgtatgtc tgttaatgga
    13981 atatgttctg ataataatcc ctgcaacact ttgctaacca tacctaaagt aagtccatca
    14041 ggtacaagct ctttaaccag tttaggggaa cttatggcca acttatccaa tagctgctgt
    14101 gtttcctcat agccaagtaa ttgctggctg ttttgttcca ggatcatgct taaatgagta
    14161 gcaactactg ttgatgcatc aactactgta taacctaaag tgtgtgcttt ctccttttga
    14221 ttttcattta tccacacagc atccattcca aaagccggat ctcttgttgg gattccctct
    14281 aaattaccaa atacctgtcc ggggtttatt gctaaccatt tatcaggatg aatcattgat
    14341 tcccccataa tcaccccagc caaacttaat ctgtaatgat taggctttaa atctaggttg
    14401 tcacgaacat gaacggatgg aattaagaaa cccaactctt gcgatatttt ctttcggaca
    14461 cctttaattc gcgaaagtaa tattccaccc tgagcattat cgaccatagg gatcaatcga
    14521 taacctactt ccaaaccaat gacatcgaca ggattaacat catcccatga taactcactc
    14581 agtttagagt cactggtttg tgaaaattca ttttgctctt tatcctgctg ctcacgttta
    14641 actttttcct gattgataaa taaataagca ataccggcta accctgctga taatagaata
    14701 aatgcaatat gaggcatacc aggaattata cctattagac caataatagc tgctgcaatg
    14761 acaagtgatc ttggttttgc aaatacttga gaaataactg cctgccccat attttgggcg
    14821 ctagaaactc gggtcacaat gattgccgct gcagtagaca ataagaggga gggaacttga
    14881 gcgaccaaac catccccgac tgtcaataaa atataattat gaactgcgtc attaaaattc
    14941 atattgtatt gaaatatgcc tattattaat ccaccaacga tgttaatgat taaaaccaaa
    15001 attcctgcta tggcatcccc tcggacgaat ttactggcac catccattga accgtaaaaa
    15061 tccgcttcct gagctacttc tgttcgacgc ttgattgcct gttcctgatt gattagacct
    15121 gcattgagat ctgcatcgat agccatttgc tttccgggta aagcatccaa agtaaaccgc
    15181 gcacttactt cagatactct ccccgcccct tttgtcacta caacaaagtt aataacgaca
    15241 aggataataa ataccactat acctactgtg taattacctc caatcaccac ttcaccaaaa
    15301 gattttatga cctgaccggc agcatctgta ccattatggc cattgagtaa gactactcgt
    15361 gtagaagcta cattgagagc taatcttaac aaagttgcca caagtatcac tgtgggaaac
    15421 accgcaaaat ccaggggtct ttcactataa ataactgcca acaaaacaac taaagaaaga
    15481 gcgatattaa aagtaaaaaa tatatccagt aggaaaggag gcagaggtaa catcatcatg
    15541 ctgatcatga caatcagtat taatggagta ccaaaccctt ggcgcatcca atattctata
    15601 taattcataa cattgttcat tcaatctcct cctctgcttc gcgagccagt tcaggtggaa
    15661 tagggacatt ttgtaaaatt tcaggcttat aatcataacg ttgcctgtca cgtagctgaa
    15721 aaatataggc cagcacctga gccacagcaa catataaccc tctgggaatt tctgcattca
    15781 gttttgttga aaaataaata gcgcgagcta gtgcaggaac tgaaataata gggatcttat
    15841 gctctttagc aaccttggat atctgaaaag caaccaggtt tttacccttg gcaatcacca
    15901 ctggagcctt ttttcctttc ttttggtaac taagagcaac tgcatagtgc gtaggattag
    15961 tcaatatgac gtcggcttta ggtacttcac tcatcatccg tcgtcttgca gtctcctgct
    16021 gtgcgcgacg tatagcgctt tttacctcag gtttgccttc cgtctctttg tattcatctt
    16081 ttaattcctg tttagtcatc ttaatcgatt tactatgttc atataattgg aatggaacat
    16141 ctattgccgc aattaatatc agacttgcag aaatcagcac aaaagatttt aaaaggatca
    16201 ttaccccact agtgattgct atttctaatg gtgctttccc caactcaagc aacaagggta
    16261 cttgcgatct aagcactaga atagatgcgc tagccaccag gacaaatttt aaaaaagcct
    16321 taagcatctc aacaaatcct ttgagagaaa tcatgcgctt aagcccttta agtacattta
    16381 atcgagaaaa ttttggctgt atagactgtc cactaaaaac ccatcccccc ataagcaatg
    16441 gtgctagcaa agacaaaata aaaataacaa gcaatagtgg aacaacagac caaagccctg
    16501 attttgttaa ttgaaacaaa ttttctaaag aattattccc ggtaacaaga atgtcacgat
    16561 cgaaatcaaa cgcctgttgc attatactgg caatttgcag tgataattgt ttcccaaaaa
    16621 taaagaaacc aagtccggta aataataaaa tgaccgttgc attaaaatct ttggaacgtg
    16681 caacctgccc cttttttcgt gattctttta atcgcttgga agaaggctgt tccgtttttt
    16741 cttgtgattg ttcttcatcg gccatcagcg caatattcct gttataaatt ggattccatg
    16801 cttaatgctc tctaccattt ctgtcccgac agtgggcaac cctattttaa tgatgacaaa
    16861 ccccatcaat agcgtaatcg gaaaacctaa agagaaaagg tttaattgcg gagcaacccg
    16921 agccataact ccaaaagaca agcttacaat aagcaaagat aaaatcgcag gtattgaaat
    16981 caataccgct tctttgaaca tccaccctga aaacataatc gtactccaga caactgatgg
    17041 atccagagaa gatttaccga ttggcattat tctgaaactc tcgattagag tttcaagaat
    17101 agccaaatgg ccattcaatg ccaaaaatat taatgtggtc accattaaat acaattgact
    17161 gattaaaggc acactagcat tgctggttgg atcaaccatt accgcaaatc ccaaacccgc
    17221 ctgcatggat ataatttgcc ccccaagaat gaatacttga aatactattt gcaacacaaa
    17281 tcccattaac aaacccaata ataattcctg tacgagatag acaagataaa ggccattaaa
    17341 attttcgaaa gacaagttat gaggcacata ggtaaaacac acacagctta aagtaaaaac
    17401 aaagagaatt ttaatgcgtg ccggtaatag tgctgaagat aaaactggca ccgtaagcat
    17461 caaaccacca atacgcacca tgggccatat aaattggctc attaagcgaa tcatagtttg
    17521 ataatcgaga ttcatttttt atccaatcaa ataaggaata ttgcttatca aagtatctgt
    17581 ataatttatc aatgtcttta ataaccaagg tcctgctaaa attagaacca aaaaggtgac
    17641 aaataacttg ggtataaagc ttaaacttgt ctcattaatt tgagtcgcag cctgaaacat
    17701 cgatataatt agtccaacaa ttaatcccgg taatatcaac actgccagta atagcaaaat
    17761 gatataaaca ctatcactaa acaacgaaac gactgattca ggggtcatat agcctcctag
    17821 gcaagagcaa aacttgaagc aagtgaaccc aaaaccaaag tccatccatc aaccatcaca
    17881 aacaacatga ttttaaaagg taatgaaata atcaagggtg aaagcatcat catccccata
    17941 gccatcaaaa cactggacac aacaagatca ataattaaaa aaggtaaaaa tagaataaag
    18001 cctatctgaa atgcggtttt taactcgcta gtaatgaaag ccggaatgag aacactcata
    18061 ggaatctcat ctaaactttt tggtacattg tgagcaaatt tttcaaataa agataaatca
    18121 ttttttctcg tttgatttag cataaactga cgaagcggct tttgtgcatt ggataatgcc
    18181 agagaaaaat caatcctttc attcatatag ggctgtattg ctgtttcatt aattttgttg
    18241 aatacaggtg acataacaaa ataagtcata aatagagcaa tgccgattaa tatttgattg
    18301 gtaggcgtct gagccatccc tattgcctgc cgtaaaattg acagaacaat aattatcctt
    18361 gtaaatgacg tcattgccat cagcaatcct ggcaatacgg tcaataaagt cataaaaatt
    18421 aaaatttgca gattgatact ataagattgc gaaccatcag ttccaggttg cattgtaaca
    18481 acgggaaagg ataaagggtc tgtgtgacct gcaacaggta ataaaagaaa taatatcaaa
    18541 atcagtaacc aacttttaat tttcatttat gattttccta ttgctgattt cagcacatcg
    18601 gcaaaagacg atttttgatc agcatcaaac ccttcaggaa gttgattgcc aaaatcacat
    18661 attagattaa tggaatatga ccctacacct attaataaat atctgtcccc aattttcagc
    18721 aatacaacct tttcctttgg acctaaaatc atacttgcaa cagtttggaa tcctttagat
    18781 gaagtaaagt ttactctatg catacgcttc actatccaag ataaaaaaat aataatgaat
    18841 aatacaaata agagtcccga aatgattctt atcagttcgc catagctgat cgtgtttggg
    18901 cttaaagtag atgaccaacc gaccgaactg attagcgaaa gactcaaaaa tgcaagatgg
    18961 agtactttgc ttttcatttg agacgtttta ttcgttcagc cttgctaacg atatcagtta
    19021 atctgacacc aaatttatcg ttaactacaa cgacttcacc atgtgcgacc aaagtaccat
    19081 tgactagcac atcaagggga tcaccagcca gcctatcaag cgctacgatt cctccctgat
    19141 ttaattgcag taaattacga atagtcattt tggtacggcc gatttcgaca gtcactgaga
    19201 caggaatatc taaaattaat tccaatttct cattgccagg atctggattc agtggattcc
    19261 cttgatttaa ttgctctgtg ctggtttcgt tcacttgagt catccttatc ccctaataac
    19321 gtattatttt aataactttc aaagctcgct tttcattggc actgcctaag gttgcagtaa
    19381 agcttggtgt cccttctata tccagagtta cctcctcgtt tatttccaaa gggacaaaat
    19441 ctcccacttt ccaagccatt acctgaccca gagtactgac tgtttccgcc atagaagcac
    19501 ttaccgtcaa ttcaacgtcc atgagttctt ctttcagaga attaatccag ttaggatcaa
    19561 tttcatcatc aggtcttgat gcccctagct ccaattgctg tttgataggt tcaagcatgg
    19621 agtaaggcaa aataaagtaa aaagccccgg tttccttacc aaaattcaag acgaaccgcg
    19681 ccacaagcaa catttcttct ggctcggcaa tattaactaa ttggggattt gtttcgtcat
    19741 tgaattttgt tacatccagt tgaattattg gttcacaggc atgaattaaa ttagttacca
    19801 gttttttagt tacgacctcc ataacacgaa gttcagtagc agtaaaatct gttttatcct
    19861 tttgagcacc aaactggcta tttccgccaa aataataatc gaccaagtca tacacaaagg
    19921 tactgtcaaa caaaataatt cctttaccac gaagaggctt gaatttatag atacctatca
    19981 ggctaggatt aggtaaactc ctcataaact ccttatgctt tgtaataagt agcggatctt
    20041 gtttaatttc aaaatctcta gccgttaaat gataaatatc tgctgcaaac agacgaactg
    20101 ccctgtcata aattttgtct aataccggaa gctgtccttt aacaatacgc tcttgtccgg
    20161 taaaattaag tgttttgact ccttcttccg ctactttttt gtcagaatca aaattagagt
    20221 ttaattcctg gctggtagta tcttctggtt ttttacgaac tggctgatct cttgaactca
    20281 attcttcatc attcgtttca tcatcaattg actcatccac tgaatccagt aatgcatcga
    20341 tctcttcttg tgataacaca tctttctcag tcataagcaa tccattcgca tctcaaccag
    20401 attttcaaag ttattttaaa attgaagtgt tgattagttt aaaattagct caatcaatca
    20461 gtaacaacta tagaatggtt atgtttatgc aaagcccgtg ccatccaaga aatatatcaa
    20521 taaatcaaat agatagtgta ataatcaaat cttgattagc tcatttaatt agactccgcc
    20581 aatgttttga cgtaaaaatc ataaggttta tattgatagg acttacgaaa aactccaaag
    20641 tcgaggcaga aaatgttttg gcgagaggat ttagataaac taaatgactg aattaaaaat
    20701 attttttaac aaagagattg gggtttttca taagtcttat gcacagtatt tgaggttgtg
    20761 agcgttcctt aatggtagtg aaatcacttc aaaaataaat gtttagaaaa tcctccactt
    20821 agtctactgc ttgttgctcg catttatctt cagctaaact ttgacaattg gagttacaat
    20881 ctatttcatc agaggttaaa caaacatcat ccaggcattg ctgataattt tccccaacac
    20941 attcttccat atttaattta tcagtgccct catccagacc aggaatatcc gcggcaaatg
    21001 ctatcaagga aaaaaataaa aagcaggcta tgctggcaag attgaatatt ttgttcatta
    21061 gaataccttc cattgatatt tgatttaatt atagacatcc tatagccttt gttcctgttt
    21121 ccataataaa aaaatcatgc gacactcact caactgtcta tttaaaaatt tctgatatgc
    21181 gcagcaaaag aatcttatta actaatttta cccgcgcaat ttatagtgaa tcctgttgta
    21241 taaggccaga cacagaaaaa gaacttattc actatattgc acaaaatcag cctaaaaaca
    21301 tgctaaccag gggtgcagga ttgagttaca gtgatagctg tctaaatcaa gacggcctgg
    21361 taattgatac tagtcgcttt aatcatttca tcgattttga tgtcgaaaca ggcatcgtag
    21421 tatgtcaggg tggaacacca tttaaggatc tgtttttact tcatgatgaa ttcattcccc
    21481 cagttctccc aggcacagca tttgccactg tagcaggtgg aatagcccat gatgttcatg
    21541 gtaaaaataa ccattcagcg ggaagttttg gacatcatat ttcttggttt gatttattga
    21601 taggcgatca aatcatgcat tgtagccgcg aaaaaaatag tgatctattt ttcgcaacta
    21661 tagcaggttt aggtttaaca ggcattatta cacaagtcgc tattcgcctt aaaaaagcac
    21721 cacgccatct ggcaataaaa aataaaccat ttgcatctat ccaagaatta attgcagaaa
    21781 tggcgactta tggtcttcag caagattacc aagtagcctg gttagacttg ttatactcag
    21841 aacccagagc cattttatca acagcaaatt attgtgaatc gccaaactat cctgataaaa
    21901 aattaaaaat ttatagcctg cctaaattac catttggact tatcaaaaaa tggaacatga
    21961 aagtatttaa tcaatatttt tactccagac aaaaagaaaa tgaaatgatg agtctcaccc
    22021 aatacaataa tcctctggat aaaatatcac attggaaccg actctacgga cctagaggac
    22081 ttatacagtt tcaagccgta tttgatcaag ataaggcagc gcaaattatt aatcacatat
    22141 tagagattat taggactcat agtgctactc caaccctcac tgtccttaaa ctatttacac
    22201 agcctgggtt aggtttgctt tcgttttgtc ggcctggatt tactctggct attgatttta
    22261 ttaataatcc tcacgccaaa aaagcaataa gcgcgatgaa tcaattcatt gcagaaaacg
    22321 atgggtgtgt ttatttggct aaggatttat tactaaatcc agaacaatat tttacaatgt
    22381 acgaaaatca atcgaaattt gctcaaatac ttacaaaata tcgatgcccc atgcgttcag
    22441 atcttgcgac acgtttagga atcataaaat gatgcagagg aaatgggttg ttttaggagc
    22501 tacttcagtt attgccgaac aattcgcaca ttttgctgcg caagccggta accatttatg
    22561 tttagttgca cgcaactccg agcaattagc cttaatctca aaagatattc aattgcgata
    22621 taaaatccct tgtaacgcga taactatcaa tttatccgag cctaacgagc aattgcttac
    22681 cattttagaa accagagaaa gagaattgga tttatttatt gcgcattgtg attttaccga
    22741 taataaccat ttaaatccgc aatcgattaa acatttaatt gataccaata tctcatctac
    22801 agcgatcctg attaatacct atttacaatc ccaacaagaa aaacatcgca tcgtttattt
    22861 aagctctgtt gcttcctgcc gcggccgtgc caaaaatagt ctttatggtg ccagtaaagc
    22921 agcaattgaa atatttctcg aaggcctgca gcaaaaagca actccacatc aacatatctt
    22981 aatcgctcgc cttggataca ttgatacaaa acaaacgtat ggactacccg gagtttttta
    23041 cgcagctcaa cctcatgact gtgcaaaggc atgttgggaa aaaataaatc ataataaaag
    23101 attattttac tatccttctt tctggaaagt ggtcatggca attattacaa aactcccttt
    23161 tttcatttat aaaagaatgg gaaaaatata agtatcatta tcgcaatgat ttgcttttat
    23221 gcgttataac gccctattat aggcttttgt ataatgataa aaactatcct agtattaatt
    23281 taagtaatat tatgattggt tttaaaataa tgaatattgc cgatttgcaa tcttttcttg
    23341 cagttgtaga actccactct atctctttag ctgccaaaaa attgcatatt actcagcctg
    23401 ctttaagtaa aagaattaga taacttgagt cggagtggaa tacccaactt tttatttctt
    23461 caggattaag gactgagcta actgataaag gaaaacaatt attgccttat gttcggcaga
    23521 tggtttatct gagccatgag ttaattagca gtactggtac agataaaaaa aaacctcagt
    23581 tactgctaaa tattggtacc acagtatata ttgctcaaac tattatcccc ccccttataa
    23641 tccatatgaa cgcactggac ttgaactact tcattaatac gaaactgatt gctgacaaag
    23701 atgtagctca cggtttaagc gaaggtgctt atgatctgat tatctctccg ttcatgagta
    23761 attctgctga aattaagtct gtacctttgt ggcgagaaag gctcatccca attgtcgggt
    23821 tatcacatcc tctagcaaaa attaaagaaa aactatcctt tgctgaatta gcagaattcg
    23881 atgcggtgct tatggaaaaa aactttataa tacgccgaat tattgataaa gaagctgaaa
    23941 aacaaaagtt gactctcaaa ataagagctg aagccaatat tatttataac aacatagctc
    24001 ttgtcgaaca aggtatggct tggagtatga ttaatgaacg cttattgaat cctaatttaa
    24061 aacctctcga actatctgat tatgccctca gcattgattt ttattgtcat tttcttcaga
    24121 aacgtacaga tgaacgattg attcgtattt ttattgataa tctggtcaat tgggttaaca
    24181 cgtcaagtga attaagctct ttttcccttg ttaattagat aaacccttta ttgccagtta
    24241 aaaatggggg gattaccgtt tagaaaagca tcaggacgct cttttaggta taattttaat
    24301 gcatcagcat ctattttgta gggattgcaa gggcgacctg aaccctctag cttccctgtt
    24361 tgtgctctca gacgcttcca gttttttatt gtagtggtcc ctactgaaaa caatctactt
    24421 actccttcta acgtgcctcc gttatctata tgccgtaaca cgttttctca aagtctaatg
    24481 aataggtcat aaattaaaaa gcgtcataat aacaaattac ttgggcattt caaattttaa
    24541 atcagtatag ttcattggcg taattatcaa accagacggg aggcaaaaca agatattctg
    24601 gattacatta ctatgtttta taacaatcaa agattgcatt cgtctttgga ttatctaagt
    24661 ccgaatcaat ttgaaaatcg gtactggggg ttaatgaaaa aaggggctta actgggatgt
    24721 gcaaattgga ttgaccactt catgatttag tcgattagga gaatgctgcc tgatctattt
    24781 ccatcataca taacttttaa ctgtagctca tcgaccattc atattcccaa tacatccttt
    24841 cagtcacgat ctaaagaata aaattttgtt ccatacttta ggaggataca acagtaatag
    24901 cctacatcga tagcaaaaac cagcagcaac tctcttttag tcatttacgt ttgccgaaaa
    24961 cgtcacagtg cattcagaat atttgattat ggaaattaaa tgcttactcg gtcagatact
    25021 gattttagag aatcaattaa attttagtca attacagaat taattaactg aaaacctatt
    25081 tattaaatat tgtttcaaac aaaacatcaa caaaaaccta ttaggtaatt gaaaaataat
    25141 atttattact agaagtgata tgagatatca agatattcga ttggtcaact aataatcaat
    25201 atgtttaaat ttatttattg cattaaccaa gagcatatca tgtcaattac tattcatact
    25261 tggaaaggag gtactaaagg attaggcttt aaaacccctg taaaatctac tttatttggt
    25321 ggtaatgtgg gacacgctgc tttagaatta acttggccag ctaataaaca aggagatgaa
    25381 ttagcaaaga aatacgcaca agcccatggc gtaataatta gtaaaagaac tgaaattgta
    25441 ggagaaactc aagaagaagg taggatcatg ccaaaagagc gagtaaccta ctttgcctat
    25501 ttcagctggt ggccaggaga gcaaaatggc cattatatta attctttcaa cgacgataag
    25561 gacgctgaat ggagacacga gcccgataaa aaaatgcatg ctgattggaa aaatcaaatc
    25621 tatggcccac agggaatgag tgaagaaaat acaacagacg ttaagggaat actgattaaa
    25681 aataaaaaca tacaaaaatt aaaaactatt cagcacagta ctctgacagc tgatatagct
    25741 caggataaag cttataatga gcttctacaa cataaagaaa aattagacga agaatataaa
    25801 tcattacatg cgaaagcagt cgcatatcaa aaagaaaagg aaaatgctat acgagaagaa
    25861 agagaaataa atcctaattt tgaactgctt gacgaagata tttctagaat gggagatctt
    25921 ccaagagagt tctcaatttt agaagcgcaa ttgaatctat gcatcgcaga ttttaatgaa
    25981 agacatctga gttcaggaaa aaaacctgat tcaagcgtga ttttacctac ctctatggat
    26041 gaaaccagtt taaatcatac cttagatacg gaaagcattt tacgagaaat gatcactcta
    26101 gcgaacagtg aaaaagctta caattttacc aaatttaatt gctctacctc ggtttgccat
    26161 attgtgaaag caggagtgga tgaaaaatta aaggaaaatt taagcgataa tgggtttaat
    26221 ataaatcgtt atgtgacttc ctatatcacc actccaacca acgtaaatgc tttaggatta
    26281 aaattacaag atgcgctgct taagcttgaa ctagcagaac atacaaagga agcagaacag
    26341 gtacaaaagc cagtggctaa tacgataagt aatcgtttta atgaatttaa gtcaagactg
    26401 caagccgtag tatcggagcg gaatgcgcat aaagatgaca ccgaagaagt aacgattaat
    26461 aatcataatt ccctgaggta gtgtttaaat gtagtagttc aaacttgatc tgacagttac
    26521 cggtttttca agaagtgtct gtcaggtcaa atttagttta taaatttttc catccagatt
    26581 tgtttatcct ctaccaaagt ttgcattgga gtacgaccac aacacatttt accgcccaag
    26641 agatctcaat taaaaactat tgaatttaag tcagctttgc actatctaag ccactatatt
    26701 cattttcagt aacttcgcct tcttcctgtt cctctatcaa ataggcagat gaagaactgt
    26761 tgttaccaaa aattcctaac tgaattctga ctgagttact actgcttgat gatactcctg
    26821 attgttgggc agcatgagta cttacagcat cctcagagcg cgatatcact ctaatttctt
    26881 cttttagctt cgcatggcca tggttcattt ttttaatttt gcttgttaat ccagaaattt
    26941 ctatattcag tttttctaat tcttctgtta atttttctaa ctgtctattt aattcctttt
    27001 tatccttctt tttaagagag gaatccttcg attgagtttc tacagtatca atctgggctc
    27061 tcaacataga tgcttgcagc atcaaacttt tcataggagc cacaactatt cttctggatt
    27121 cacttcccat atgcgttaat ttttcaacga tagtatgacg agttttagcc aagtgctcta
    27181 atttatcggc atgcctttct ttaaaaggga ttccgctatc aggatgcgca ataaaacatt
    27241 ccaggatggg ttttaactga ttagtaaatt gtattaaacc cagtaaatct tctgcagggt
    27301 ataaaccaaa ttcattagta aaatgagggg ccataataat agcaagattt tcccatgtca
    27361 tgagattagt ttcataaaaa acccctgctg tatgcattag gtaacaatag tggtaaagaa
    27421 tttctccaac acgttgatga tcaagtattt ttgaaagcaa taaagagtca ataaacttac
    27481 tcagcaactg tactattgcg tcggtatttt caacattatt cttctcagaa ctcaatagat
    27541 tttttaattc tgtcataaaa agcttgagca atgggtcttc agttttcaac aagacacttt
    27601 ctttaagaac taaaggcaac attcctaaaa cattattcag atgtaaatta tttatctggc
    27661 catcttctaa aatataacac gctaaattcc ccacactaaa attgtcatta ataacttgct
    27721 caagtaactt ttctgactct tctctagatc cagccaatcg aaatactcct ggccagttta
    27781 atagctctgg attttgtgac actaattggg caacacatcg aaatacattg acaatcacat
    27841 gagtactata tggtggtggc ataacaaatt cccagattaa attttgaaca aaatatcatg
    27901 ggatcaaaac taaatcaatt attgttaatg aaatacgaac ctgaattttt ccagccacat
    27961 taatgtcttt tggattaatt gaattcatct caagtgttac ttgcgatgaa acttgtgttc
    28021 cagaataaaa tcattattgt ctaatcagga acggcctatt ctattaggcc gttcctgatt
    28081 aatttgagga agcaaaaatc actttaaaat tttaatcatt ggcaatattg tttttattac
    28141 cccaaaaaat tgcaaaatat aaatgattaa aataattaat accaatacat ttaacagact
    28201 tttaatagcc ccgggcatag gtataaaaac attaattaac cataggccta caccaaaaac
    28261 cactatcact gcgattaaat tcagcaagtc attcatcata gtgctcatta gtaaaccagt
    28321 gtctatatta tagcaaatcc aaaaacaact tgcttttctg cagtcgaaaa gaaaaatctg
    28381 gaaatattaa attttttcaa tcatttcctt tatttctttg atcttactgt tcaaatcttc
    28441 aggaacaccc ccaccacctt gagccatatc atctcgacca cctcctttac cacaaagatg
    28501 tcttactaat tgagcagcac ttggtgcttt gccaataata ttcttactca ccccggcaat
    28561 gacattcatt ttattctgtt caatagtaaa taatatgatc accgctgaat caattcttga
    28621 ttttaattga tccatggtat ggcgcatggt ttggctatcc ataccctcta actgcttaat
    28681 caataaatta atacccttga tctcctcgat ctcaccaaga atatctgctc cagatttttg
    28741 tgctttttca cttaaaagtt ttgcaatcat tttttcttga tttttattat caagcaataa
    28801 ttgagaaact ttttcctgta gactattagg tgttgtttta agggtagctg ccagattatt
    28861 cataaatccc agctgttcat tgacccaggc taatgcataa cgacccgtaa ccatctcaat
    28921 ccgcctgata ccgctggcaa taccatattc tgctacgatt ttgaaaagac ctatatcacc
    28981 agtacgtcgt gcatgagttc ctccacaaag ctccttggaa aaatcaccca tggataatac
    29041 gcgtactgcg tcggagtatt tttccccgaa taaagcaaca gcaccacttt gcttggctga
    29101 ttcaatgtcc attacttgag ttattacttc attattagct cggatctgtg cattaactac
    29161 ctcttcaatt tgctgaattt gttgaggagt caatgcctcg aaatgagaaa agtcaaaacg
    29221 tgccctttca gcatccacca gggatcctcg ttgttgaaca tgttgcccta caattttttt
    29281 aagagcagca tgtaacaaat gagttgcggt atgattcaat cgaattgcat cccgtcttat
    29341 gttatctact tgcgcatgga ttaataaatc taaagtgagc tcacctttaa ttacttttcc
    29401 ataatgtacg acagcttgcc ctactttttg tgtatcatcg acttgaaaag taaattcttt
    29461 tcctatgagt aatcctttat ctcctacctg accaccactt tcagcataaa atggggtatg
    29521 atcaagaatt acagcccctt ttgctccctt attaatagat ttcacttcat taccttcttg
    29581 aagtaatcca ataatctttg cttccatgga ttctttttca tacccatgaa attcagactg
    29641 atgatccaat tgagacacgg cgtgataatc tgtagtaaac tgactggcgg cttgagactg
    29701 ttctctttgt tgttgcatca attgattaaa tgcttccata tcaatatgta aaccttgttc
    29761 acgaatgatg tctgctgtca aatcgatagg gaaaccataa gtatcataca acttgaatgc
    29821 cacttctcct gatagttcct ggccttgcaa atttttaata tgatcttgta ataaacgcaa
    29881 tccttgttcc aaagtacggg taaactgatt ttcttcttgt tgcaagatcc tttcaatatg
    29941 cgctttcgaa ttgattagct cgggataagc atcccccata acatcaatca aaggctgaac
    30001 aagtttggaa aaaaatgggc tgggtaagcc taatttattc ccatgtctga ctgctctgcg
    30061 gataatcctt cttaatacat atcctctgcc ttcattacta ggcaataccc catcaactat
    30121 caaaaaagag caggaacgaa tatgatctgc aatcactttt aaggaagtat gatttaaatc
    30181 aatatcctgg cccaattgtg ctattgcttt aatcaaatac tgaaaactat caatttcata
    30241 attactatgg acaccttgaa taactgcggc taatcgttcc aatcccatac cagtatccac
    30301 agaaggcttt ggcaaggggt gcaaatgtcc ctctctgtcc ctgttaaact gcatgaatac
    30361 caggttccat atttctatat aacgatctcc atcttcatct ggactgcccg ggggaccacc
    30421 tgctacttca ggaccatgat cataaaaaat ttctgtgcaa ggaccacatg gacccgtatc
    30481 ccccattgac cagaaattat ctttttcccc acatcgagag aatcgctcgg gagatacctt
    30541 catttccttt agccatatgt cttctgcttc caaatcctct ttatagacag taacccatag
    30601 ccgttctgcc ggtatgtgta aaacttctgt taggaattcc caagcatatt gaatagcttc
    30661 gcgtttaaaa taatcaccaa aactaaaatt acccaacatt tcaaaaaatg tatgatgtct
    30721 cgccgtataa ccaacatttt ccagatcatt atgtttacca cctgcacgca cacagcgctg
    30781 tgctgtagca gcacgctgat atgaacgagt ttcaagacct aaaaaaagat ccttgaattg
    30841 caccatacct gcattggtaa atagtaaagt gggatcattt gaagggacca atgagctaga
    30901 agctactatt tgatgaccgc gttgaacaaa atagttaagg aatgcttgtc taatttcaga
    30961 acttttcatt ttttaatatt taccagttaa attaagtaag aagatttaac ctctttaaat
    31021 acctttgaaa taacatccct atcaaaccct cgatacagca aaaaccgttg ctgtttttgt
    31081 atttcactat aagagaaatc atcctgtctc ttaaattttt tctcccatgc ccgcaaagca
    31141 taattgaccc aattgtcttt ttcttgatgc agcacacttt gaatgagatc tggatctatt
    31201 cccttgtttt ttaattcctg cctgatcttt aaagggccat agccttgatg aattcgtact
    31261 ctgatataat tttctacaaa acgcacatcg ctttgatacc caagacgttg gcattcatac
    31321 aaagcatttt gaacatcatt ggtgctaaat cctttctgct tcagtttatc acataactcc
    31381 atagcgctat actcgcgcct ggttaacaga cgcaaggcgc tatcgaatgc tttagtcatc
    31441 tatagtctca aaaagatctt ctgatgaact agccaacaca gataactttt tttccaagag
    31501 ctcagtacgg atttgttgtt caagctctgc agcgacttgg ggattttctt tcaaatataa
    31561 tctgacattt tctttgccct gcccgatttt ttcctgtttg tagctatacc aggcacctga
    31621 tttctcaatt aaattcaatt gaacacccag attaataatt tcactctcac gagatatacc
    31681 ttcgttatat aaaatatcaa actcagtcat tttaaacggt ggcgctacct tatttttaac
    31741 tactttaact cgggtttcac tgcctaatat ttcttcaccc tttttaattg aaccaatacg
    31801 gcgaatatcc aaacgaactg aagcataaaa cttcaaagca ttgccccctg tagtagtttc
    31861 agggctacca aacatcacac caattttcat acgtatttga ttaataaaaa taaccagtgt
    31921 gttcgaacgt ttaatgttag cggtcaattt acgcagggct tgcgacatca atcttgcctg
    31981 taaaccaaca tgggaatcgc ccatttctcc ctcaatttct gctttgggag tcaaggcagc
    32041 aaccgagtca attattacga catcaactgc tgcagaacgc accagcatat cagtaatttc
    32101 cagagcttgc tcgcctgtat ccggctgaga aaccaacagc tcatccacct tcacaccaag
    32161 tttctgagca tagcttggat ccagagcatg ttccgcatcg ataaatgctg ctgtaccacc
    32221 cattttttga cattcagcga tcacttgaag agtgagcgtg gttttaccag aagactcagg
    32281 gccataaatt tcaacaatac ggccttttgg caaaccgcct atccctaagg caatatcaag
    32341 acctaatgaa cctgtagaga ttgcttcaat atcccgtgat accgtgctat ctcccatacg
    32401 cattacagac cctttgccaa attggcgttc aatttgcgaa agcgccgctg atagtgcttt
    32461 ttgtttattt tcttccataa tttattccag attataatgt gtgcaagaac aaaattatca
    32521 cacagatgtg agcgagcagc aaacagtaat cttagtgtat tactaaataa aagtagcata
    32581 cgatagctga tgattagtct tttattataa gctacgtgga ataaccattt gttcaaaaat
    32641 gaaggtgttg tcagtttgaa cttcaaacct tgatttttag cttggtaacg gggcaatata
    32701 aaaatatata gacaatccga cataaaaacc aaggttttac aatcaaaata acgaaaatgt
    32761 taactactca taatgctgtg tcataattta ttgaccttga gatgagtaag caatctggga
    32821 acaaaaataa cagcggctaa aactatacca ccgaaaaata ataaattgct tgttgaaaag
    32881 aaaggggtct tattatcaga cacttcagtt gaaggttttt tttctgtcat aatattttcc
    32941 tcatctacct tattgaccaa agagagagat tgaggaacag aagcggtttc cttattttct
    33001 ttataacgac caaacaaagt atttaattta agatcatggt cttttgcctt tgatagtgcc
    33061 gattcatagg ctaattcggc gtcgaaaact ccaagactcg actgttgcaa aaattcttca
    33121 ctatgctgat gcaatttgtc tatttcattt ccgaattcaa catatctggt agttgcattt
    33181 cttcttaata cattgagctt gttatgtggg atgctatcat caaaccccat atccggattt
    33241 tttcttacct ctcttgccaa atttaaaatg gaataaacta tctgaacact atcatgttga
    33301 cattgctgtt gaatttcagt gcttggttgt gaaaaacctt tatcatcaaa actcttaatt
    33361 tctgaaagcc tgagtaattg ctcttttgtt ctattgtaga ccgtgtcttt agagttattt
    33421 tcaataagca gccattctgc ataggatctc agatattctt gctccacata aaattttact
    33481 tgctcaaaaa cacgatcaaa aatggtcata tccattgagt cggattttga attggtttct
    33541 atttccttta ataacttctc cgcgaatcct aataattcat tcacctcttc gtatatgaga
    33601 gcggcgttta ttatcgcatc ttcacctact ttaagttttg cgagctcaac atatttttta
    33661 atctggatat caatcagatc acataaaaac tcaaacaacc ttgtatattg actcatattt
    33721 tttcctattt tttaatcaaa taaattatta tacttcttgt gcaactttta ttattcagtt
    33781 taaaacaaga aacacacttt cgaggatccg tagattttca ttgtataaat ccactctacg
    33841 aaatatttgc gttacagcta taaacaaaag acactgttta tgcaagagat ttattgtaat
    33901 ttttattagc tgaatggtaa aaacaagaat ttgagactaa ataatagaat tgaaaaaata
    33961 tagactattg atgttttatt aaactatgtt tttaaaaaac cattcggtta gccacagact
    34021 tttttaagag tagaaagaac tccatttaaa gcatattgac aggcagacaa acgtatttct
    34081 tgccgacttc cagtgaattg ttgtcttgat gtttgttgaa attgatggtg aactgcccag
    34141 gcaaagcata cagtacctac tggtttttca atactgcctc cttctggacc tgcgatgcca
    34201 gtaactgata cagcgatatt acccatacta tgttgtaacg caccaacagc catagccaaa
    34261 gccacttctt cactgactgc cccatgagtt tgtattagct cgtgagcaac agcaagcatt
    34321 tcctctttag ctaaattact ataagtaaca aaaccacgct caaaccaatt tgagcttcca
    34381 ggtatttccg taaggcaagc agcaattaaa cctcctgtac aagattccgc agtcactaat
    34441 tgccattttg ctttttttaa ataggcagct atttcttcaa ccagtattga gaaatgaccc
    34501 atacctttca cctataaaaa accccataaa acatctatgg ggttttttga taatttataa
    34561 ataacactga aattaatctt actggccttg gccttcagta gaaggttggg cttggttatt
    34621 tccagtattt tcaccacctt gttgttgctg atcagttggt tgttgttgct gttgttgatc
    34681 agtctttgta tctgcaggct tcgagccctc accacatcca gttaaaaata atgctagaca
    34741 actagcagca gcaatcatag ctaaacgatt cataatcact ctccctattt attttttgta
    34801 atatcacgtt aattctagca aatattctca ttaagtgaag aacttatctc ctgaacttaa
    34861 aaaataaaat ttatatttta ttgcagtatg ttaaattaaa tgacataaac catgccttag
    34921 taatcatttt ggcaaattct atagtaattt gcagatttta aaaatcatac ttatttttta
    34981 gacacatcaa ggttatatta aatgcttttt gatcacattg tctttattgt tttcataaaa
    35041 atcaatacca ccaacatgcc ttactgactt cgcacaacac ttttgttaaa attggggcaa
    35101 tttcaatcgg ctcaatcact atggctagct ctcacacacc aatgatgcag caatatttgc
    35161 gcattaaaac agactatccc gacatgcttt tattttatcg catgggtgat ttttatgagt
    35221 tgttttttga tgatgccaaa cgcgcgtcac aacttttgga tttaactctg acacatagag
    35281 gacaatctgc tgacaaacct attcccatgg ccggcgttcc ttatcatgcg gtagaaaact
    35341 atttggcacg attattaaaa aaaggggaat ccgtagccat ctgtgaacaa atcggagatc
    35401 cagctaccag caaaggacca gtagaaagac aagtaacgcg aatcattact cctggtacag
    35461 taacagatga agcattatta gatgccagaa aagataatat tttgttggcc atacacaccc
    35521 aaaaacaaaa aattggtatt gcatgggttg atttaggtgg cggtcgtttt catttgcagg
    35581 aattaacaga agaacatcag ttaaatgcag agttagtgcg cctgcaacca gcagaactcc
    35641 tctgcaaaga atccacgccg cttccttcat tttgttctaa ctttgctgtt aaattcagac
    35701 caggatggga gtttgatgca agcaacgcac ataaattatt gtgtgaacaa ttctccgtga
    35761 ctgatctttc ggcctttggc gaacaaaatt accccactgc cttgattgca gcaggcgctt
    35821 tattagctta tttaaaaacc actcaaaaac agtcattacc acatcttaca acacttactc
    35881 ttgagcaatc cgaggattac ctgcaactgg acgcatcgac tcaaaagcac ttggagcttt
    35941 ttgaaaatat acatggtggt ggtgagcact gtttgctttc catattagat aaaacagctt
    36001 gtgcgatggg aagccgttta ctcaaacgtt ggctgggaaa accattaaaa caacatgcaa
    36061 tcattcaaac aagacaacaa gccattaagg aaattatttt tcttcaacaa gacgtttcac
    36121 ttcatcagct gattaaacaa tgcgccgatg tggagcgcat agtatcacgt attgctttaa
    36181 aatcagcgcg cccacgtgat ttggtttcct tattgcaaac tctgacactg cttccagcaa
    36241 tccatgacga attacaagaa aacaagagct tgttaatcaa tgaaatcaag aaagaaataa
    36301 gccctctccc actgctgcag caactgcttg aaacagccat tatagacaac ccgccaatgc
    36361 taattaggga tggtggagtc attgctccag gatttgatga agaactggat gagttgcgta
    36421 atttaagtag caatgctcat gaaacattag taaaacttga acaggaagaa aaaaatcgca
    36481 caggattatc cactctaaaa ctaggttaca atagcgttca gggattttat attgaactgt
    36541 caaaggcaca agctcaaaat gcgccgcccc attttcacag aaagcaaact ttaaaaaatg
    36601 tggaacgtta tattactccc gaactaaagc tgttcgaaga caaggtgctt tcagctcaat
    36661 ccaaagcatt ggctcgcgaa aaatggctat atgacaattt gcttgaagaa attcagcaat
    36721 acatacctga gttatcggat cttgcaaaat ctctggcgca attggacgta ttggtcactc
    36781 ttgctgaaag agcacaaagt ttaaattgga attgcccgaa tctcgttcct gaatcaggca
    36841 ttatgataca agccggacga catcctgtca ttgagccatt attacaagaa cggtttatcg
    36901 ctaatgattt ggaacttaaa ccaaatcaaa atatgttact aatcactggg cccaacatgg
    36961 gcggcaaatc gacctacatg aggcaaaccg ccttaatcgt tctgttggca catattggaa
    37021 gttttgtacc tgctgataaa gtgacattag gtccattaga ccgaatcttt actcgcattg
    37081 gggccagtga tgatttatcc tctggacgtt ccacttttat ggtggaaatg actgaaacag
    37141 ctcaaatatt aaggcaggca accagccaaa gcctcgtatt aatcgatgaa attggtcgtg
    37201 gtaccagtac ctatgatgga atggcattag cttatgcaag ctgtgccttt cttgcgtcaa
    37261 caataaaagc ctatactttg ttttctactc attacctgga gttgactgaa ttacccaaag
    37321 aattctcttg tattcgcaac gtgcatttgc aagcatccat caagacagga caaattgttt
    37381 tcttgtaccg tgtcgaagaa ggttgcgcaa atcgtagtta cggattagaa gtcgcagaat
    37441 tagctggcat acctaaagaa gtgctaaagc tggctcatga acatctgaat cagatacaag
    37501 atactcaatc tatcctggtc cagactcaaa ttataaagcc tcccacatct ccagttttaa
    37561 ctgaactaaa aaaaattgat ccagatagat taactgccaa agaagcgttg gatttaattt
    37621 ataagttaaa acacctggag tgtgccgaga gcatcaattg atgtagttat tacatgaata
    37681 ggtttgcata tttaatcata ctaatcattt tgtattttcc attgtttgct caggacaaaa
    37741 aggccgggct gcttgagatt gatggtataa aaggaaaagt tcttgctaat gtggaaactc
    37801 gtttaggtga attgagccaa attaaacctt taagccaatt taccccaact gaacttcaag
    37861 accaaattaa taaagccatt caaccatttg gatatttcaa tgcggaaacc agtattaata
    37921 acctcaacaa taaaataata ataaaagttc aacctgggtc tcaaattcgt attgcctcca
    37981 ttaaagcgat gctaacagga gagggagcac aaaatccatt attacgaaaa actctgaaag
    38041 agttacctct gcatattgga gatcctctgt tttcagaaca atacgaaaaa gcaaaacaaa
    38101 atattataaa taccgcagaa aatatgggat atttacacgg cgtatttaaa aaagctgaaa
    38161 tattgataga tgaacacaaa aaatcggctc aaatcaccct gatttttgat acggggcccc
    38221 aatactattt tggccaggtg caatttgacc caacctatat ttctcctcag ctattacatc
    38281 gtttcgtacc atttgatcct ggccaacctt atgccacaga tcaagttttg aaattgaatg
    38341 attatttatc gaacagcggt tactttagtt ctgtactggt aaaaccccaa attactgacg
    38401 cccaaacagt accggtcatt gttcatttgc aacctgtacc taaatattcc tattcatttg
    38461 gccttggcta tggaacagac acaggagtgc gtggaaaagc agctcttcat gtgattccag
    38521 tcaatcgcca aggacataag tttaatgccg tagcgcaagg ctctttcagg caaaacgcct
    38581 tacaagccca atatgtaatc cccggaaaaa accctgttac cgatcaatat gccttgaccg
    38641 gtaatttttc aaatttaaac tataatgcag gttatagtaa tgctactctc ttatccctga
    38701 gccaattgca taatgtgaat caatttcaaa gagcgctttc actgaatgct ctgtatgaaa
    38761 gttttcatta ctctctacaa cccaatacgg atcagttttt gctctacccc aaagccaata
    38821 tcactttcag taaaactaaa aatctcttgt tttcgccatc agggtataat attactttta
    38881 acgcgcttgg ggcgaataag gctgtcctct cccatctgaa ctttggacaa ctttcactgg
    38941 atgccaaagc cgcgctcaca ttggattcac tgcatttgcg cctgtatggg cataccatcc
    39001 aaggtattac agcaatcaat gatatcaatg aattaccgct ttccctggca ttacttctgg
    39061 gaggaactga caaccttaaa gcctatagtt ttaactctat cggaccagga aaaattatca
    39121 cttatggtgg ctttgaaatt caaaaagaat tcaaaaaaaa ctggtatctt gttggatttt
    39181 acgatgctgg agatgtttac aatccatccg ttaaaaacat tcaatacgat attggaggcg
    39241 gtctgatgtg ggtttcccct attgggccaa ttaaagttgg attagcccaa tcagtcgata
    39301 ataagatgga gagaatagga cacaatccaa gattggttat cagtatggga ccagacttat
    39361 gatgatatta aaatttataa aaaaaggatt ttactactgc ttaataacag caatatgtct
    39421 ggttagtttg attatctttt tgatttcaac taccccgggg ctatatgcag taattaaatt
    39481 aactcattta tatcttcctg gcactcttaa aatacaccat cttaaaggac aattacttga
    39541 ccaattttct attggtgaaa tggaatatca acatcaagac actaaaataa aaatttttaa
    39601 cctggcggtt aactggcgtt tcagctcatt gcttcgcaaa aaattgccta ttgatagcat
    39661 gagtgctgaa aagattgaaa taaatcaaaa gacgcccata ctgatactaa aaaatatcaa
    39721 attaagcggt tatttaaatc aacaattaat taaactgaat acactgcaat ttaattattg
    39781 gaatcaattg attaccagcc aattagaagt tagcaactca tttcctcatg cattttcagg
    39841 aaaaattcgt ttaaacccac attctacaaa taagaatata ttctcaggag gggtagacat
    39901 tggcggtaat cggaatcaaa ctcaatgggg aggagaattt gaaggtcctg gaaaaataac
    39961 aattaacggc agtcttcaac agttaaaaca aataaaacaa atcataaaat ggcgtgactt
    40021 acattggcaa gacaataaaa atcacaattt aaaaagctcg caaggaagaa ttgaggtatc
    40081 gggaacaatt ccaaatttag ctatcgactt aaaatccaaa cttggcatag atcaattaaa
    40141 aaattggcaa atgaacgctt cgattaaggg caagctgcct tggcaatgga gtgtcaatgc
    40201 tacgttctct caaccattag atttctcatc aaaacttgat ggtttatata caaaatttgc
    40261 tttgaaaatg gaaactcaaa gtgaccatca tgccaacttt ctgatcacga ttgctccagg
    40321 ccattatcaa atgacagaaa acaatctgat gccgttaatt cagtttactg gcggcaatat
    40381 taaaggctct ctttccccaa aacaattcag tggacaagga gttctctttc tagatgcata
    40441 taaaaaagtc aatttcaatt ttaaattccc ggagtttaat gtctaccaag atatagcgca
    40501 gcaaacaatc gatgcgaaac tatccattct ttttaactcc ctggattttt tgcaaaatgt
    40561 cagtccttat ttcatgaatc ccaaaggaca acttcttgcc tccattcatg caaagggaac
    40621 tttaaataaa ccaagaattg aaagtaaact cactttaaac aaaggctcag tgaacatccc
    40681 tactttaggc ctcaatataa ctgatatggg attagctgtt tttagcaaaa aagatggatg
    40741 ggaagcaact ggctccattc attcatctga aaaaaaacta accctaaaag gacaaggtag
    40801 tttcaataag gaacttggtg gaactgtttc aatagaaggt tctaattttc cggttgcaaa
    40861 cactaaggaa tttcaggtta atatatctcc acagttaaat gtgcgcttta ctccatcact
    40921 atgtcaaatc actggaacca tccaaattcc taatgcccat ataaaaccac aaacgtttac
    40981 caatagtctt gtggctacag atgatattgt atttacgagt aagaatactc aaccgattac
    41041 cccactcttt aataccaaca tgtcggtacg agttgaaatg ggagaagaag ttgaactgac
    41101 ctttaaggga ttgcatgctt atttggcggg tatagttaca atcaatcaat tatcgcaagg
    41161 acccataacg gcaaatggtg agttagctgt caaaaaagga gaatataaag cctatggcca
    41221 ggatttatct attgaacaag gacaattaat ttttacaggc ggtagaattg ataacccaga
    41281 aattaatcta agagcatcga agaatatatc caattcattt ggtagtgtat caggatcctc
    41341 tgagctgttt aattttaata attataataa cctcaatgtt ggcaacaatt ttaatgttgg
    41401 ggtcgaagta gctggacgat tacaatcgcc taaaattcaa ttattctcta accctccaac
    41461 actttctcaa gcagatatcc tatcttttct tgtaattggc cgcccagcca atcaagcaga
    41521 taaggcaaaa ggccaattac ttttaacagc tatttcctcg atgaacttag gaacaggaac
    41581 taatggaacc caactgctgg agcaattaaa acaaaaatta ggattcgatt ttaacgtaca
    41641 aacgacaacc aattttaatc agcaaaccaa taaaatcagt gaatcaaccg gcgttgtggt
    41701 tggaaaatct ctttcggatc gaatttattt aagttataac gtcggtttat ctcaaaccga
    41761 tcctaacgta ttaaccttaa aatacatact aaataaattt ttaagcattc aggtaagcag
    41821 cagtgattca ggtaatggcg tcgatgtcct gtatactaaa agctcgtcat cgccgaaaaa
    41881 aacaaagcct gacaaataaa acattaatcc atattgtcta aggatgtaat aaatgaatga
    41941 atataatttg cctttgaggt tatctcgttt aagaaaaaat gctatgtcga gagcattagt
    42001 aagagagacc aggctgcacg cacaacaact gattgctcca ttgtttatta gtgaatattt
    42061 aactgaacca aaagaaatcg gcgctatgcc aggccaattt caactcggcc tcaattgtat
    42121 ccctgaagaa atcgaaattt tatcaaaatc aggcatccct gcagtcatct tattcggtat
    42181 ccctaagcat aaggatgctt atggcagtga atcatttaat agtaatggaa tcattcaaca
    42241 ggctataaaa aaaataaaat ctgttaatag tgacatgtta gttatcaccg atctttgctt
    42301 ttgcgaatat accgatcatg gtcattgcgg ggttctgaat gggactcaaa ttaatattgc
    42361 tgccacttta gagttgttag gaaaacaggc ggtaagtcat gctgaagcag gtgctgactg
    42421 ggtagcaccc agtggcatga cagacggaat ggttttagct atacgccaag ccctggatca
    42481 tgcttcattt catgaaattc ctattttaag ttatagtgcc aaatattgct cgtgccttta
    42541 tgggcctttt agagaggcag cgcagggtgc accacagttt ggcgatagga aagcgtatca
    42601 aatggatccg gcaaatagtg aggaagctgt aagggaaacc cgattagatc tggaagaagg
    42661 cgcagatatg attatggtta aaccagccgg tttttatctt gatattattt ataaacttaa
    42721 acaacatttt gctgatgtac cgctttgtgc ctatcaggtc agcggtgaat attcgatgat
    42781 aaaaaatgca gcaaaaaata atctaatcaa tgaagaacaa gcaatgactg aaagtctcat
    42841 tgcgattaaa cgtgctggcg ctgattttat tatttcttat tttgctaaag gcatggctga
    42901 gttattaaac tctcaaaatt ttatttaatt atgagtcaaa taaatcaaat caagaactat
    42961 aaaacgacta tataaagaga aagacttgcc tcatctataa caaatctcat atagtaaatg
    43021 ggattggcat tgcctaatca actacaatct agaaggagaa atcaatgaat ttaatgctta
    43081 aaggtttatc ggccttagca attagtctcg gtgctgcaac aacttttgca gctccagctt
    43141 atttaacaac ccataacaga actggtgaag aatccaatgc ctacatagca ggcagcatcc
    43201 cctctcttta cccaacagca gcttactcta ccaatcaagt gtattggaat ttagtgagat
    43261 tggcttgcta tggacataca actaatggtc aatgccctgc cttaattaaa atggcaacca
    43321 atacagctaa cccaattgat atcggatacg ttaccatgga tctgaatact ggcgatatta
    43381 ctcccaagac gctatcggct aaaggctatt cgctgagagt aataggacct ggagaggctg
    43441 aaattactaa aaactagtca attgtctcca tttgttttga atagcttatg taggttgaac
    43501 gctaatacaa cctacataat caatacatgt aaatacttac gtttatctta attaagatta
    43561 attatctcag cacacgatta tctaaacgtt atttatcccg acacacataa aactaacagg
    43621 gcttatacaa aaccccaagg tcagtgcaaa aaatatttcc taattttagc gaagagattg
    43681 ggatttttca taagttctga ctaataaaaa agccacgcat atcacgtggc ttctaacaag
    43741 gtcaagagaa agctttattt agtcggtgct ggaggttctt tatcaatagt ccaagttgct
    43801 tgcacaccta ctaaattggc acctgcagtt ccagatgctg taacgttata tctggttgtt
    43861 gcagtaaacg catcagtcct gttaataact ggatcacttg aagtaaataa atgggtatat
    43921 ccgatatcga gaccgatatt attcgttgcc tgataatgag cgccagcaga taaagcccaa
    43981 cgatctgtat ccggtaaacg cacgtctctg tcaatattgt tcgtaggtgt tggatcataa
    44041 ccaccgccca cacgaagcat taatttatca ttaacatgat aattggcacc aatcgctacc
    44101 cgcaaagtat cgttataatt ctgcggtgat atgcttatcg ccttggcttg ggaaacattt
    44161 ccattatccg ctggtcttcc ggcagctgca ttagagagtt gtactgtttt ataactactc
    44221 catgcagtat aaactaaaga gcctaacagt gcaagtttgt cattgacatc atgataagca
    44281 ctaatagtca ctacatcagg aaaatcaatt gagttactaa ataaatcatc gctttgaaat
    44341 atgccaggtg ccgggaacaa aaatggcgct aatatattac ctggacttgc caatggccct
    44401 gttagacgac tgtagccata gaattcatgc cgcattgatg actgatagtt tgcaccgaga
    44461 cgagtatgat tatcgttaaa catgcccaga acacccacat gaaaacctac tccaaaagag
    44521 tgtcctttat tgtaagtcag tgaatcggct aacatggggt taaaaggtgg caaaacattc
    44581 agtatgttag gtactccaag tatacgattg tatttaactc tagcatattg caaatccaaa
    44641 ccagccccca aagcaacgtg ctctgttaat ctaccaccaa tttcgggtga tatattggtg
    44701 gtaattaact cagtaaaagt ggcttgatag cgcactggag aatctgggcc ccaatctgtt
    44761 gccaatccaa atggtgatac aacacttaac ccaaacgtag cattttcacc caatggcaag
    44821 gcataatgaa acgagggcac aaaagcatcc tcagccccat tgatattatt gaaaacctca
    44881 gaataaacaa aagaagggaa agggggcgga ttaattgttg agtaagtact aactccgctt
    44941 aacttggcag ttgggaatac accgacacct gcaaatacag cttgctgatc gcggattaat
    45001 accaaacctg ctggattata ccaacctgta gaagcatccg ctgcttcagc agccacaccg
    45061 gcagcgaagt tacctgttgt atatccagct ccttcggtat acaaagaaaa tccacctgcg
    45121 tttaatgtac cagtagctag aactccgaga acagcagtac tcactagcgt tcttaagggt
    45181 ttgtgcctaa
//
LOCUS       DACTJE010000005        42567 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.005, whole genome shotgun sequence.
ACCESSION   DACTJE010000005 DACTJE010000000
VERSION     DACTJE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 42567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42567
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.005"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..896)
                     /locus_tag="I9760_000199"
     CDS             complement(<1..896)
                     /locus_tag="I9760_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT5041999.1"
                     /translation="MVLEFDTIILGGGKGGKTLAMDLAKSGQKIAMVENNQIGGTCIN
                     VACIPTKTLVQSAKVAHYCRKAKDYGLNTTLHPIDFKAIRARKDAVVKGMREANLKQF
                     LDSGMDLMLGHGHFIGPKMIEVTLSSPRDKQKTLHITADKIIINTGALPFIPPIAGLD
                     KVNYFTNDSLMNTDLVPQHLLIIGGGYIGLEFAQMFRRFGAEVTVIEASSEFLGREDK
                     DIAEQVFQTLSNEGIQFAVDTKINAIRQEQTEVIIEANRQGQSEIIRGTAVLVAVGRI
                     ANTAGLHLDKTGVELDERGFIKV"
     gene            complement(948..4307)
                     /locus_tag="I9760_000200"
     CDS             complement(948..4307)
                     /locus_tag="I9760_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HAT5042000.1"
                     /translation="MTNSWKDKLQKQLTQQFGSPKGKQLIDKYTETMSMSYSEQHTPE
                     EAIQDILQIEKLSKENPLVIDFYENTHSQYPLHIKLYRYETPIPLSDILPMLENMGLR
                     TYTERPYKIKTRDGQLFWISDFNVTYSQTDSINITQVKDIFAEALISINLGICENDGF
                     NKLVLCAGLSWQEITILRAYAKYLHQIGIRYSQSYIEKTIEMHAAIARELIHFFYLKF
                     GLKRKSAIHKDILALEQKIQINIDAISSLDEDRIMRYFWSLMKATLRTNYFQKNHAGL
                     SKDYLSFKLKSSEIPDLPPGQPLYEIFVYSTRFEGIHLRSTKVARGGIRWSDRPEDFR
                     TEILGLMKAQKVKNAVIVPSGAKGGFVLKKPLHAASREQIQQEVISCYKSFINGLLDL
                     TDNLINDKTIPPQNVICYDDPDPYLVVAADKGTATFSDIANSIAKEHGFWLGDAFASG
                     GSAGYDHKKMGITARGAWESVKRHFRELDINIQQQDFTVVGIGDMSGDVFGNGMLYSK
                     HIRLLAAFDHRHIFLDPNPDPVKSYEERLRLFNLPTSSWEDYNLQLISKGGGVYKRSS
                     KSIPISPQVKKALAIDANALTPNELIRALLKAPVDLLFNGGIGTYVKATTESHADVGD
                     KTNEFCRINGAELHCKVVGEGGNLGFTQLGRVEFALKGGLINTDAIDNSAGVNCSDHE
                     VNIKILLDKEIREKKLTEKKRNQLLSRMTDEVADLVLQDNYNQALILSISAAHSSHYS
                     GLYQDYMKELEKWVNLDRSVEFLPDDKKLLERKSTGAGLTRPELAVLMAHTKIHIANE
                     LLKSHLPDDSYFTTFLETGFPISLRKPYAKALPKHPLSREIIATQLSNKLVNNMGITF
                     MYRMLMETGMSIADIACAYTISACIFQTDVLQNLIDSFQDKISLSTQYELLHHIRQLL
                     NLATRWFLHNNRLTGGIEKNIKNYGKSVKKLELLIPKLMGGVTKKYLEKLISQFVSIG
                     IEKDMAQKIAITRALYTSLNIIEVATQNEFDLSLTAETYFKVGSQFNLVWFRDQIAND
                     SREGHWNTMARLSLRDELDNLQRRLTIAILTTNTKERTSEKLINYWLEQNHPIQKRWE
                     KLLEMLLGSENIDYTIFFIALRELSSLIQVL"
     gene            4508..5233
                     /gene="ceg9"
                     /locus_tag="I9760_000201"
     CDS             4508..5233
                     /gene="ceg9"
                     /locus_tag="I9760_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg9"
                     /protein_id="HAT5042001.1"
                     /translation="MYKNILFFSNFSRMRWNEYDGDRYQSLWEHNKRDIKHTFFGTIA
                     NGKDLREVLFQPVTASFSMLVAGAFAQLLVPYSIFMLCMGEAGFAGRTLINYLEITTV
                     ALAMPFIALLSTVGRCIATVKCLVEDQYNLAEMVEDKEVKPNKIYLDCAEVNYTFRDE
                     NNNQFSRKYYKFQYKMRGLNDELCQGSFTFRDLERAPDNDDFNGKLSSLTLYKYDILD
                     LIKRVHKDKFEEMEDENLIRMSI"
     gene            5465..6418
                     /locus_tag="I9760_000202"
     CDS             5465..6418
                     /locus_tag="I9760_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042002.1"
                     /translation="MRVFYGLLGFMIFNLVACEKMALLTTPPKKQQLSNSPLAARAER
                     YFWRTLHEGRYQDIPQADYLLMAAYLENPYDSKLAAHLGFIHIWKITERERVKNNSPL
                     LPNEIILSKKYFADALQLDPENPIYQGFYGDTQLVEGQIFKDKQEEVRGYFTLQKAIS
                     NWPEFNYFTAGYPMSSLSADSDHFKKGLDWQWKTLDICAGEKISRQNPDFTPYMKRET
                     TIGAQRACWNSEIAPHNFEGFFMNMGDMLVKSGDPETGIKIYNNAKLTKNYNKWPYKD
                     MLEKRILNAKANVQHFNQKSNDPNQSIMFNSGYGCVVCHQR"
     gene            6578..6919
                     /gene="arsC"
                     /locus_tag="I9760_000203"
     CDS             6578..6919
                     /gene="arsC"
                     /locus_tag="I9760_000203"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212942.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAT5042003.1"
                     /translation="MEKITIYHNPRCSKSRQALEILQNKGFEPIIIEYLKTPLDLEQL
                     KRLRTHLALEDLVRTNENVFKELGLSLANEAQILEAMVNEPILMQRPIVTFKGKAVIG
                     RPPEKVLELFD"
     gene            complement(7006..8160)
                     /locus_tag="I9760_000204"
     CDS             complement(7006..8160)
                     /locus_tag="I9760_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HAT5042004.1"
                     /translation="MMFGFLNMTLWGYLLAGFVLTHITTTAVTIYLHRYQTHRALTLH
                     PLVSHFFRFWLWLTTGMTTAEWVAIHRKHHANTDMEGDPHSPQVFTLKKVFWQVPELY
                     REASKDKDMIKKYSSGTPNDWIERKLYSPHANKGIFLMLTLNLFLFGIPGLTLWAIQM
                     MWIPLVAGVVNGIGHYWGYRNHESSDSSTNVFPWGLLIAGEELHNNHHTFASSAKFSI
                     KWWEFDLGWFYIRCLSFLKLAKVKKLPPKLIIDKNKSHIDVDSVKAILGNRFQVISDY
                     YKLVIFPTLRDMQYRIKNRDLKNARHLFKKPNEQLSHADQLRLKTLLKQHDNLQAVHN
                     YRELLQQIWLKTSLSNKDLLNLIQKWCSQAEESGLHVLRQFASQLKSYSL"
     gene            complement(8279..9523)
                     /locus_tag="I9760_000205"
     CDS             complement(8279..9523)
                     /locus_tag="I9760_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAT5042005.1"
                     /translation="MSFKQLALIEPLNRAVSELGYTTPTSIQLKAIPLILNGHDLLGS
                     AQTGTGKTASFVLPILQKASQQTQTSPNRVKVLILTPTRELAIQVHESIIQYGKYLTL
                     RSAVIYGGVKSHNQIKQLDSGLEILVATPGRLLDLYQQRAVKFDEIDTLVLDEADRML
                     DMGFIHDIKKIIKLLPLKRQNLLFSATFTPEVRALARNILNKAVEIDIAPRNTAVKTI
                     KQTVYSVDRNHKLALLSHLLHKNNWGQTLVFSRTKHGANKLVKQLAESQIYSVAIHGN
                     KSQAQRIKALADFKSGKVQTLIATDIAARGIDIEKLACVVNFDLPHVPEDYVHRIGRT
                     GRAGASGLAVSLVSTEEIKLLLSIEKLINQKLERIKIKDFEFLHNFSDFVSAKIQKFA
                     PPKAGYKGNISRKSRTAFNKSA"
     gene            9855..10130
                     /locus_tag="I9760_000206"
     CDS             9855..10130
                     /locus_tag="I9760_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAT5042006.1"
                     /translation="MKQKKIYVGNLPFGTTEEALSIQFSQYGKIEEMFLIKDRLTGQM
                     KGFGFITFSAQQEAESALEMNGQPFDGRSLKVCMAQKKTNSVSRSRW"
     gene            10306..10983
                     /locus_tag="I9760_000207"
     CDS             10306..10983
                     /locus_tag="I9760_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAT5042007.1"
                     /translation="MKANGFFDEQSSLKKSKIGLNLVWMFSLGHIFLITLSNVLVQYP
                     FDMMGFHTTWGAFTYPAIFILTDLTTRIATANKARKIIMLSMTPGLILSYLVASYIEA
                     GNNLNESGLSVLHLMPLRIALACFIAYVVGQLLDVAVFQRYRNRPAWWLAPTLSAVAG
                     NFIDTALFFTIAFYHCSNPFLSQNWPEIAMVDVFFKIMISLIAFVPVYGLVLSILGIK
                     TTNKLMV"
     gene            11021..11659
                     /locus_tag="I9760_000208"
     CDS             11021..11659
                     /locus_tag="I9760_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5042008.1"
                     /translation="MLTGLRLLTKDLKILNGKYIQLEPITSQHREELRKAANHEQIWQ
                     YMPQKATGKLFNSWFDDCLEKMSIRDQTTYAVRCKKEGDLKGATSFYDIQPENKRLAL
                     GYSWYIPKVWGTTTNPESKLLMLHQAFDAWGINRVELGTDSRNIHSYRAIKKLGATEE
                     GVLRQHMILHNQVITDTIMFSILFSEWPAVKERLIQRLNYAATDKDHLAVSI"
     gene            11794..12321
                     /locus_tag="I9760_000209"
     CDS             11794..12321
                     /locus_tag="I9760_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042009.1"
                     /translation="MYNKMFKPLDTDPILYFKMYSNYTEGRVDDCCAFILMPSGLQRD
                     WVCLQSIQFAFNKCGDVLGINIIFSGNESNIHKKVRETMEGMLKLKLQYGRGEELFVF
                     DEEKKTFHLGILPGKDTQAYLEGIIAFIQDSYRLQPDFAQNIKAQLLNKEYLAQEYSR
                     LRWKPPEKESVCVLM"
     gene            12476..13822
                     /locus_tag="I9760_000210"
     CDS             12476..13822
                     /locus_tag="I9760_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HAT5042010.1"
                     /translation="MYRKRWLILLCALLNNGCLWLGSEYRKPHVNTPQKWPHKYSIQT
                     EQAAYLPDLHWWKQFNSKELNASIQKALQNNHQIHLAMANIESAQGQLQQVQLSWLPN
                     LTGLAGYTQFPVLGNPGTTAIAYPAYIINIFQQYKQQKSAQAMLEASIYAQYSARLVV
                     IAQTAASFFTLIAQNEALHLYNKLLKDYRTYLKLTQSQYRSGLISLDNITQIKSRIQR
                     IEAQIKVVQHNIVVTKNALHFLFNENPGDVEIKTLFENIDSNQLVPGNLPASVLSVRP
                     DIHEAEALLKAAHADVGAITANLLPGINLGAYLGEGSNVDGAIKLGQAYLNGPIIDLP
                     LFAQVDVSKARYKAIYIKYITTIRKALRDVANDLSAYSTSSQQLNNNLSALSDEKQQC
                     HLAEVRYRHGIDDYLNLIKCQILLDEFKLVINQNKLEKLLSIVTLYQDLGGGSRGH"
     gene            13812..14849
                     /locus_tag="I9760_000211"
     CDS             13812..14849
                     /locus_tag="I9760_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAT5042011.1"
                     /translation="MGIDQQKTVRTLRLSLTCIFLFLITWYYQVPESAWTLVTIWFVM
                     YEYSTVGGVLTKSFLRFAGTALSAIYGMIVVYFCANNPLINIMALVPGLFLYAYFFMG
                     GDKTYIGTIGAVTLTIVLLNYNDIDTAVLRVFNVIIGVIGSMFMIRFFYPQYARDKVL
                     EIQLNFITQLANLLESYLNPTQSLAAVQTKYLDYERKMLDGFTLYNRYIGEAKIETKK
                     APFFIPHSIAAMQHFRRLFRLFSVFIYYLSTEEIRSDPWVCDQLSKLLRNLQSLQRKL
                     LQQDAGPIGGDVEVAEPEEEIVIETPNIENKIATEAIVNNMQKEIALLDTEIKEIILI
                     YDVYDIRLNEM"
     gene            14906..15898
                     /locus_tag="I9760_000212"
     CDS             14906..15898
                     /locus_tag="I9760_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAT5042012.1"
                     /translation="MSDKPKAGFPVIILLCVFLFILIYHYSYLFPFTNNAFVVANVRP
                     VAANVKGYITNIYVKNEQEVKKGQPLFTVFKAPYELAYRKAVSHVQEAKAQLLLFNKQ
                     VEKTKYLLQAQKELYEKFRFDYEHNYSASRDHAVSKLTVNTMLKEKNAALSKLKALEK
                     ELEVNQQQVIVQKKKIDSLIAVMKNAKIDLDETTVYAKQNGAVQDMFVALGTPIKTRK
                     PVFAIADTDTLFIQANFNETDLRRVQPGDKVSIFPRMYFGSKIYHGVILSRNWAASRL
                     ETHRATQIQIVRNSESNWFLLPQRLPVQIQITDYDPVHYPLSIGASAYVYIHTH"
     gene            16075..18018
                     /locus_tag="I9760_000213"
     CDS             16075..18018
                     /locus_tag="I9760_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042013.1"
                     /translation="MKIPFEGKWRATITKREAGFSQRVVVGSATSGNGSYNGVVGNSF
                     VFEDGWVELQWNNNAGSGWQESAVISSVGMTSPLVVVKFVSADDNFPNQRDGDYDDLQ
                     VRFEHLDAPFEVTQRPYALDRGSLAMFPDGIFDASQGIQYMGVRIKNTWFFDWQSNFP
                     ATGMKIGIAPVSRSALSAAGIMVIDSWTPQEQEGLGQIMDGGFVRVPDIKINEETTIY
                     FKLDVSNANPSKPEIGFVAQRDAYDPRYDEPTRIVDKKIFISRSSYNPATRELVTAIP
                     EGTLYLKLNKIAIDRKGATKAAQELKKCLKQHGGGIKGMSKWLHKLSRGFWQNSKDKE
                     RWCKEQGNDALRKLLKQLLDGKDIDICHLKAILDQCCERPGGDCECCPCGDGDGRPGG
                     WDDGTGVDGWCRVKPVFWLPLDFEYKVVPNPAYIGQFGPLAFEDPWWKVVLIILAIIL
                     AIASVIYDYVYASEDDQYIIGQVVRNGDAPINLVDAAVSNLNGSRGVDLGQLDAQGND
                     INNGSPITVLDSIIQLDRTAAGSYFGTLDAVLGNVVWKSGATSATTRGFISTLGTSVS
                     IPYDEHDTLSDTVTYINEVVISELAAIPQTLSDPGDSGSVWVDLATARPVALNYGSPT
                     DSSDNRAFANPIRSVIDVLNLRF"
     gene            18037..19821
                     /locus_tag="I9760_000214"
     CDS             18037..19821
                     /locus_tag="I9760_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042014.1"
                     /translation="MKREFFQKLDKILDIASEHKQMLLQYPDVVMVGVGPERRKGKIT
                     GEPAIIVTVRKKRSSNELKKRGEKPLPQTIKGIPVDVIELGKAVEAPEIIAVQEKAKK
                     TISKIRAKWLQTPNITAIGYGYKEKGGEIDFNTIAIQFFVKKKFPIAELQNQQITPIP
                     EKINGVSTDVIQLSPIRPAAASGSRGDRHDPLKGGVAIGVGSKPFHYGTYGATVFDRT
                     TGERLFLSNQHVLDASPGTDTIQPSPVLLDDSVEVGFQLDFCLPIHFIRLDTPNTTVG
                     TVLAGAAVAAAVAAALSDEIDPTRRGQEATMPATGSKTLFETQKVKMDYPELPIPGTP
                     FKVKTAWDYERHTDAGIKTFSVSEEKKNPHVLRDKLLITDKKLYHPGDTIRIFGLILL
                     EECAPASQTRDDRQPISPRELEDITHYPNLASSINLDLVATMDSDIASSPARRTNTRA
                     AVINPKRCRCDRYHPVAILTPTTEDRAFPVVLREPVFAQKNQIFQDLLTVVKQRDDEK
                     LLERVFVLVRYGCIYTGTLRVNNIPAGPWKHFFYVQTVNIATSDMKPLLAAQIIGGLP
                     VSENAKADLDIACGPLTWEDGEFDIELI"
     gene            20007..20405
                     /locus_tag="I9760_000215"
     CDS             20007..20405
                     /locus_tag="I9760_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0260 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042015.1"
                     /translation="MFGFFSDYKQYITLRNFAVVYNGLTGLAVLYSLWSNPEADPSEY
                     AIDISIHALTAITLMCKQAPESVKAVAMALNTYRGFDALFKAITSLPSTIPGIANAVD
                     VLNHRFNFKELEKLGNEETVETRSAVQHTM"
     gene            complement(20557..20847)
                     /locus_tag="I9760_000216"
                     /pseudo
     CDS             complement(20557..20847)
                     /locus_tag="I9760_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946237.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(20920..21066)
                     /locus_tag="I9760_000217"
                     /pseudo
     CDS             complement(20920..21066)
                     /locus_tag="I9760_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011789342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(21166..21804)
                     /locus_tag="I9760_000218"
     CDS             complement(21166..21804)
                     /locus_tag="I9760_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAT5042016.1"
                     /translation="MSDVLNNYYLQTMGIEVWVMRESTPSCEKHLAALADEVASCVRC
                     SLHKTRTQTVFSRGNPKAKLMIIGEAPGFYEDQKGQPFVGKAGLLLNKMIRSIEMTEN
                     DIYIANVLKCRPPNNRDPALEEISQCSSFLTRQIQLIKPHLILALGRFAGQFLLDKPL
                     PLKQLRNHIHYYDNTPFIVSYHPAYLLRNPSDKKKAYIDWLAVKNFLLDYGQ"
     gene            22061..23335
                     /locus_tag="I9760_000219"
     CDS             22061..23335
                     /locus_tag="I9760_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042017.1"
                     /translation="MNSQKDLKQLDIKQVIAWAFYDFANSSYFVVIFTFVFATYFTSY
                     IAPNTILGTELWGYTISVSALLIALVSPIVGAIADFSGHHKSWLFVFTYLGVISTGCL
                     WFAYPDSHSIPLVLTCILISNFALEVGTVFYNSFLANLAPENYLGRISGWAWGCGYLG
                     GLLCLILSLFVFVKGELGGLWFGTENFANIRAVTVFVALWISLFSLPLFLMVQQKTGE
                     HLTVGTAIRKGLQELRLTIKSLPKQRDLFLFLIARIFYIDGLNSILALGGIYAAGNFH
                     LSVSDIMVFGIILNITAGLGAGLFAWFDDWIGSKKTILIALACLTLTFCFLLTIHSNA
                     LFWVFAPVFGIFVGPVQAASRTFLARLAKPEEMTRMYGFYSFSGKATSFLGPFLVSTT
                     TALADSQRLGMAVLIPFFIIGGGLLLLVHENE"
     gene            23456..24112
                     /locus_tag="I9760_000220"
     CDS             23456..24112
                     /locus_tag="I9760_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT5042018.1"
                     /translation="MKIIISILFAFISASGHAFSCYDPQTIIQKPIQFDKKRIALTRE
                     YQLTHYGIDSESIEIEPKMIVLHWTCIPSLDTTFRIFDPPALPSSSPRRNELPGDLNV
                     SSHFLVDRDGTIYQLMPETWMARHVIGLNHYAIGIENIGGVDSKDDLTEEQAKANAFL
                     VCYLKNKYPQIKYLIGHNEYLQFKGTALWLEKDPNYQTDKTDPGPTFAKKVKQLVLAS
                     "
     gene            complement(24167..25708)
                     /locus_tag="I9760_000221"
     CDS             complement(24167..25708)
                     /locus_tag="I9760_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HAT5042019.1"
                     /translation="MTNTTDFYSITPLDKARWQKILINKITVRTLGKIFVFIMAITIM
                     ITLFNFSYATDSKLKPTVITVSEEEFQVDGKGKKTKEFKLTFTHPDGTKDNKGYFANK
                     GDMFHVLVENKSASPITLHWHGLIVPSDQDGVPDVSQILIQPGQNKLFNYRLLQAGTY
                     WMHSHQKFQEQKQLSAPLIIYDKDDPHKDLQEVILFLEDFTYKDPQLIFDQLRTAKMD
                     MKAMDSGNDLNDVTYDAFLANKKTFAAPDVISVQPLKKVRLRIINASSSTNFKIDTGK
                     LSATLIAVDGENIEPIVDSSFPIGVGNRMDLLVEIPSAGGAFPIKAMAEGTNKQTGLI
                     LKTDNTPTPILSPNTTNKTGRVNYYELEKKLKGKNALTNKKINARLHYVLDGQMNGYI
                     WTMNKQSWPDVSPKVIHFGDRVEMIYENKSSMSHPMHFHGHVFQVTEIDGQPLDGAMR
                     DTLLVQPHSTVKVQFDALNPGIWANHCHNLYHLNAGMFTTFEYQHYPKPDFYLKTIRQ
                     SKNNN"
     gene            25937..26371
                     /locus_tag="I9760_000222"
     CDS             25937..26371
                     /locus_tag="I9760_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042020.1"
                     /translation="MKRIFLISIIGIVLNSAYAFEHLGECGAHTWHEVTSKSGSWKIT
                     LKMISQNGQFVDPSCAEIIETGLAQNNPLSYGLKFSKDADFNLAYTLTAVSEEQGFQS
                     KACVFVITANGPAQPDIQALSYHGAECLWRVVKGVGENFSVG"
     gene            complement(26386..27366)
                     /locus_tag="I9760_000223"
     CDS             complement(26386..27366)
                     /locus_tag="I9760_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAT5042021.1"
                     /translation="MHSFDSIAVEFDLKNHSMQKIAIEDLNIDYQDKDKIYWIHSDLN
                     QKNIFKKLREKLRLPEEVIQLCGEKDNRSTTIEIDEALTLQIQGLCSMKLNDNYDADF
                     GNLIIHLTPNYCFTASKTLSSVLFDLLKSCPKSLPYAKTSCFLLFLLLEGVINDYAKI
                     HLAYEELADQLDAQARTTNKNTYSQVLELKHGVMKIKRYTIAIREILMRLTSRNILVI
                     SEQCRNSLYNLSNHCHLIVNEIDSLRDILNGLLGQIDNQLMQNMNETMRVLTAFAAIF
                     LPLSLITGIYGMNFYWMPELGWKYGYFWALGLIVLCALLLFLIFRKKKWF"
     gene            27621..28217
                     /locus_tag="I9760_000224"
     CDS             27621..28217
                     /locus_tag="I9760_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAT5042022.1"
                     /translation="MNQDMEKIEKMYQLIMEINKLKLVFRNTVTNPQRNESTAEHSWS
                     ASMITLILMNQLKREFTEVDELKTIKLVLIHDVVEIYAGDVLAFDLAARKDKEKVELE
                     ALEKLSAVYPSFGIELDSLWHEFEERKSLEAKIAKAADAICPIFQRLQAKQSYIPFNI
                     SMAQLEKTKYPYFQFSKTFSNLFEKLKADLLKEQLIFL"
     gene            28358..29857
                     /locus_tag="I9760_000225"
     CDS             28358..29857
                     /locus_tag="I9760_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAT5042023.1"
                     /translation="MYDDLWKPYASIQLPSLKLGGIVLNGQLLSETPIHLVLKAFNRH
                     GLIAGATGTGKTKTMQVLAEQLSLNGVPSLVMDIKGDISGLAMPGQTSTKLVERGQQL
                     HIDFTPRGFPVELLTLTPQDLGVPLRARIDDFGPLLFSLMLDLNDTQAGVVTVLFEYA
                     KANQLNLVNLDDFKSLLQFAQTDRGNQLIAAQYGGVASTSIGTIVRKIIELESQGGKQ
                     FFGEPSFVIQDLLRTDANGHGVISILRLLNMQDKPKLFSSFMLKLLSDVYRQMPELGD
                     PDKPRLVLFIDEAHLIFNYSSKALLNLIETIVKLIRSKGIGLIFCTQTPNDIPEAVLS
                     QLGLKIQHSLRAFTAKDRKALKLVSQNFPVSSYYNTEQLLTTLSIGEALVSALDAKGQ
                     PTPLVQAMIRAPESRMGTLSNEELKVLVNQSPLFAKYYQMIDVPSAKEKLSELISEQQ
                     VAKQQTSQKENDSSVLNNLSKNTLFRQVVRQVFRELTRAILSLFKIKRN"
     gene            29939..31342
                     /locus_tag="I9760_000226"
     CDS             29939..31342
                     /locus_tag="I9760_000226"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAT5042024.1"
                     /translation="MINITGTYTDQYQLAMAQVCFLKGQHERIATFDYFFRALPFKGG
                     YAIFAGLEDLLDVLEQLRFEQQDIEFLQHQNMHPDFIRYLQQFKFNGTVYTSLEGDVV
                     FPTRPVVIIEANLIEAQIIETLLLNIINFQTLIATKARRIRQVAGERILIDFGLRRAQ
                     GAGGYFASRAAIVGGFDSTSNVRAGRDYSIPVSGTMAHSFIQSYDSEIQAFSDYAEAW
                     PENCILLVDTYNTLQSGIPNAIKIAKEMEKRGHRLKGIRLDSGDLAWLAKRARHMLDE
                     AGLNYVAITASNQLDEYVIKNLLEQDAPIDAFGVGTNLAIGAPDAALDGVYKLAFING
                     QPRIKLSDTPAKITIPYQKQVYRVFERDGTYRGADVVCLKTEKKIDMMHHPFEILKSL
                     SIKHYKKEALLHKVMENGKRLLPKKSLGEIARFSQQCFDRLPLEYKRFINPHIYKVGL
                     STELKMKRDQLIAEHSR"
     gene            31347..31967
                     /gene="pncA"
                     /locus_tag="I9760_000227"
     CDS             31347..31967
                     /gene="pncA"
                     /locus_tag="I9760_000227"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAT5042025.1"
                     /translation="MKTLIILDVQNDFMPGGSLGVPNGDAIVPVINSILHCFDLIVAS
                     QDWHPSNHISFASNHTGRKPFEKIKLGNLEQTLWPDHCVQGSVGAQFHPKLNTNSIEA
                     IFRKGTDPNIDSYSGFYDNLHKKTTGLAGYLREKGARELYFCGLCADICVYFTIKDAL
                     SQGFDCYLVEDATQALVKDDFIKIKKELIKQGSHIINSSNLLNNLQ"
     gene            complement(32050..32433)
                     /locus_tag="I9760_000228"
     CDS             complement(32050..32433)
                     /locus_tag="I9760_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAT5042026.1"
                     /translation="MSIQLNHTIVWVKDRDKSAHFLTEILGLPEAIPYGPFMVVEMSN
                     HVSLDFCKTDKEITPQHYAFLVTETEFDDIFKRICERKMDYWADPGQTRLGQMNYNDG
                     GRGLYFNDPNGHLLEIITKPYGSGS"
     gene            complement(32433..33647)
                     /locus_tag="I9760_000229"
     CDS             complement(32433..33647)
                     /locus_tag="I9760_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HAT5042027.1"
                     /translation="MYPKTKLSFMTLLLLISFASVNAVLFTPALPSITAFFRITEHAA
                     QQTITCFLVGYAIGQLVYGPTANRYGRKKTLYIGIGLQIISSLLCVLAGMISMYPLLV
                     LGRFLLALGSGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSH
                     YGWESCFYAGTVYGAILLAMVRQLPETQKNLQLEAFQMKHLLQGYVSQFKNKPLISGG
                     ALMGGASCFVYIFAATAPFIAINLLGMSTAEYGAANILPSLGLTLGSIWSAQFAKKYS
                     QRAGIQLGILISGFAALAMMMAALTHHSALIKLFFPMIFIYLGLSFIFANASTFAMSH
                     TSDKAHGSAVMNFINMGLVTVVILGLSLLPMSQYLLPAIYIILCIFMMWLFFSFIKSN
                     EIISSIDRNKES"
     gene            33765..34628
                     /locus_tag="I9760_000230"
     CDS             33765..34628
                     /locus_tag="I9760_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT5042028.1"
                     /translation="MGKFDQITYFIAVVEEHGFAAAARKFGVSTAAVSRQIARLEAEL
                     KAELLVRTTRKLSLTDIGARYYQQCKKTLLELNEAEIAIANSRNEAVGILNVTSSRYF
                     AMRFLLPYLPEFMELNPKLQIKIELAERFPDLAQENVDVLFGVSMEGPPELVRKRVST
                     TRYVLCASPDYLAKNGIPKTPSELGKHRYITHSMRNPDNLVTFKDNEQIYVEPVLWLN
                     DSRALCECALLGMGIIKLHEYIVTEALQDGRLIEVLPEFQESSLSVYLYYQASRYLQP
                     KIRRFIDFFTK"
     gene            35048..38398
                     /gene="sdbA"
                     /locus_tag="I9760_000231"
     CDS             35048..38398
                     /gene="sdbA"
                     /locus_tag="I9760_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbA"
                     /protein_id="HAT5042029.1"
                     /translation="MHKKYNYYSLEKEKKTFWQHILDILKAPFRLPGWVVSFFLARNI
                     THVALNPNNIPQQRLIHLTKTSNRPEDDIVVINFKKRPPHKWFNDTLIKIANTIAALP
                     FVTPRLRTRLHYDNENDINHVNKLLAEIDALVQGKSKQKYCKGRAFDWSKIHLKGLEF
                     LDPKMRGYVYEQLHEKYGYVSYTTKRKPNIEFFTLKTPDGSELDSVQVTGEDEEKKPM
                     GERKFIITCIARDQNFINWIKDLNYTAKNLGATAISFNYRGVDYSRGLVWTENNLVDD
                     ILAQVQRLISLGADPKNICLDGMCIGGAVATIAAAKLHEKGMKVKLNNERSFTSLSSL
                     VFGFIVPELQTANWWSPLTYGRFLLAGVVYALLTPLIWLAGWPVDVTKAWNRIPAQDK
                     MYSVVRDKDNGLYDGVIHDHFCSIASLVDSQINSILYKLSTDQPLTEEEKQILCDDQF
                     SHHFKPSQSVLKNPKYKGPHFISRQDLVAELGHREEYTNHDYFLDRLREKFQLDRATR
                     PVALAEDGEKDIDGISSQLSNNKERPLIIASSGGTGHISATHGIINDLQSKTDNVVIT
                     QHHAELYKNKPFSITSVLIRIGVWFTSLPILEDILKGVMRFIGYPVLPSSSIFWDQMS
                     KIQQSETKKENGIETGRTRPYVDMLLDIYPEGYEYTAFNNATHLTSSIEDIQTMISFK
                     GHVEEDNRNIVYQNILQRLMHAAKQNTPYTRLISTQALSLGAICDAVKYYNTVFLPVY
                     NAERGTSYQPIAIDQYMTDLPSLGCIHFMNNLEELTSEQRQLMEIHAVNMSEPFKEAH
                     FGKEQGFKAVHNIDPRNNPMIRNAFKDPSLTKYLDKTQSFDLHFNVYKKEKQNALPVL
                     NGKEKITIKPHAKIASIMIGSLAANASADYAKYLLNQGYEHIFLFGGLNDSIAARIDQ
                     IINSYPAPTRDEIRKKIILLGNQSDVEMAPIMTRSNCVVIRGGGLSVMEQMAMPIMDD
                     KIVLLHHEDNEEGPLTSGLSWEDGNSDKLIEYLSEKGAYAKKTSPGLCSGHLHEAEKS
                     FEKKYHGQLKSTETKKKVDLTIPQQETYSLKKEWDRKTGYTESGHILSHQHRFFNTIP
                     EVREPFCSKEDLHHNELSSQSLVSVSAG"
     gene            38556..40055
                     /gene="legG2"
                     /locus_tag="I9760_000232"
     CDS             38556..40055
                     /gene="legG2"
                     /locus_tag="I9760_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegG2"
                     /protein_id="HAT5042030.1"
                     /translation="MINKSRLLSLIACFNNREINMPKILDSHAYREMENELNTLFEQI
                     YKHGKNDRTAITLQAQQFLDNLSESIRDHSRLLLTKIAREELIYFKEKKPGDSREKFQ
                     NIDKMSDYFNNLSALINFSVMQQEDPTLRAFYYDMYIQLMNFCYLKGDLFGAGAIYTG
                     LIANNLPNSIDWKQLSSESRLIFKDCEEKFKRLFNLSTTYNAHTDLKRQFKTALIPAL
                     PSLLTVQIYTKDSYDNSVSDFERIQDKLRQLDIEHNRFLNQLQNDRSTWSKAYYDYMS
                     NVYLPQSIFEYTTLLREYEELVTTHGGPIAKQFHKDKNSEPLVNFVESTLSENQSVLK
                     VSPFQNERIVEMLSEIDVVKSECSSPAVFDDLSWKLREMNCTLVKAKSIGDYTSKIFA
                     NLDTPYMEENTDEDPKPVLVEELQTDDVYQLHHSGMKLMRELEQAIEDHKPKVIYHCS
                     GEANYGFFKEARRRGRHLSREPMDIEVMDMDVMDYDENDLRDQTVMITV"
     gene            complement(40121..42343)
                     /locus_tag="I9760_000233"
     CDS             complement(40121..42343)
                     /locus_tag="I9760_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5042031.1"
                     /translation="MSTESFRILVIDDNPAIHQDFMKILNVSKNSVLLNKFDAELFDD
                     ETEQLGISLPEFEIDVATQGIEGIEKVKRSLEEGRPYPLAFVDIRMPPGIDGIETIKR
                     IWAIDPQIQIVICSAYSDYSWESTVNTLGLSDNLLVLKKPFDVIAVRQLASALTQKWV
                     LARETQKHTEFLNQLVEERTESLQQSFSLLRATIESSSDGILVVDLQGKIVDYNSQFV
                     KLWNIPESIMKKTSESQLIQYMSTQLLNSEQHAQHVKEFSIHIDDSSIQIYHFRNGKV
                     VECCSKPHRVGPQTVGRVWSFRDITEQTRLKEKLEYQATHDALTNLPNRLLLIDRIEH
                     AISSYARHKMKFAILFFDLDRFKMINDSLSHEAGDQLLCAVAKRLRSLVRKEDTLARL
                     GGDEFVMLFQSFNSEEQIAGVAQKILKSFQKPFHIAERDINIHVSIGISVYPTDGKTV
                     NTLLSNADMAMYQAKFRGGNQFSFYTEKLNKKTNQQFQLELDLQKAIIHHEFFLLYQP
                     QFTVNTHEIRSMEALIRWNHPQKGIILPLDFIPTAESSGLIVPIGEWVLRETCKQIGT
                     WRTMGLPEIRVACNVASKQLRQRDFPAMVEDLLKEYSVKPHCLELEITENVIIDKEIQ
                     RTIKKLKSLGVLIILDDFGTGNSSLNLLKQVAVDSLKIDQSFIQNISKSPGDEAIIDA
                     IIAIAQSMNFNIIAEGVETQNQLKFLKKRRCNDIQGFLMSKPIPSEEIEKLLQGNRTK
                     "
     gene            complement(42346..>42567)
                     /locus_tag="I9760_000234"
     CDS             complement(42346..>42567)
                     /locus_tag="I9760_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAT5042032.1"
                     /translation="INDNGIGIQKDNLDRIFSFGFTTKKNGHGFGLHCSAIYARDLGG
                     TLYAESNGNGKGAQFVLDLPINPKTLSTR"
BASE COUNT        12745 a         7763 c         8791 g        13268 t
ORIGIN      
        1 actttgatga atccacgctc atcaagttca acgccagttt tatccaaatg caatcctgct
       61 gtattggcaa tacgccctac tgcaaccagg acagctgtgc cacgaataat ttctgattgg
      121 ccttgtcgat tagcttcgat aatcacttca gtttgctctt ggcgaatggc attaatcttt
      181 gtatcgacag caaattgaat gccttcgttg gataaggttt gaaaaacctg ttcagcaatg
      241 tccttgtctt cccggccaag aaattcgctg ctggcttcga tgacagtgac ttcagcacca
      301 aagcggcgaa acatttgtgc aaattccaag ccaatataac cgccgccgat gatcaataaa
      361 tgttggggta ccaaatcggt gttcattaaa ctgtcattgg taaaataatt gactttatcg
      421 agccccgcaa tggggggaat gaatggcaga gcgcctgtat taataataat tttatcagct
      481 gtgatgtgca gcgttttttg cttgtcacgt ggtgaggata gggtaacttc aatcattttt
      541 ggtccaataa aatgaccatg tcctaacatg agatccatgc ctgagtctaa aaactgtttt
      601 aaattcgctt cacgcatccc tttcaccaca gcgtctttac gtgctcgaat tgctttaaaa
      661 tcaattgggt gcaatgtagt atttaaacca tagtcttttg ctttacggca gtaatgtgcc
      721 acttttgctg actgcacgag cgttttcgta ggtatgcatg ccacattgat acaggtccca
      781 ccgatctgat tgttttcaac catcgcgatt ttctggccgc ttttagccaa atccattgca
      841 agagtcttcc cacctttgcc cccacctaat atgatggtat caaactctaa aaccattcca
      901 ttctcccgaa aatttcaatg ttttatccac ttgatgatga tccttattta caatacttga
      961 attaaagagg aaagctcacg caaggcgata aaaaatatgg tgtaatctat gttttcgcta
     1021 cccaataaca tttctaataa cttttcccag cgtttttgaa tggggtgatt ttgttccagc
     1081 caatagttta tcaatttttc agaagtacgt tctttggtgt tagttgttaa aatggcaata
     1141 gtcaaacgtc tttgcagatt atctaactca tcacgcaaag acaggcgagc catggtattc
     1201 caatgccctt cacgtgagtc attggctatt tggtcgcgaa accaaacgag attaaattga
     1261 ctaccaactt taaagtaagt ttctgcagta agcgataaat caaattcatt ttgcgtggct
     1321 acttcaatga tatttaatga agtataaaga gcccgagtta tagcaatttt ttgagccata
     1381 tccttttcaa taccaatact gacgaattgg ctaatgagtt tctcaaggta ttttttagtg
     1441 acaccaccca tgagcttggg aatcaacagt tcaagttttt tgactgactt gccataattt
     1501 ttaatatttt tttcaatgcc tccggtcagt cgattattat gcaaaaacca gcgtgtcgcc
     1561 aagttgagca attggcgaat gtgatgcaat aattcatatt gggtggataa ggatatttta
     1621 tcttgaaatg aatcaatcag attttgtaag acatccgttt ggaaaataca ggctgaaata
     1681 gtgtaagcgc aggcaatatc agcaatcgac ataccggttt ccatcagcat gcggtacatg
     1741 aaggtaattc ccatgttgtt aaccagctta ttgctcagtt gcgtcgctat gatttcacga
     1801 gacaaagggt gttttggcaa ggctttcgca tagggctttc gtagcgagat gggaaaacct
     1861 gtttccaaaa aagttgtgaa gtatgagtca tcgggtaaat gcgattttaa taattcgtta
     1921 gcaatatgaa ttttggtgtg tgccattaaa acagcaagct ctggtcttgt cagccctgcg
     1981 ccagtgcttt tgcgttcgag gagtttttta tcgtcaggaa ggaattcaac gcttctgtca
     2041 aggttaaccc acttttccaa ttctttcatg taatcttgat ataagccact ataatgagaa
     2101 gaatgagctg ccgatatact taaaatcaag gcttgattgt agttgtcttg caaaacaaga
     2161 tcggcgactt cgtctgtcat tctgcttagc aattgattgc gttttttttc tgtgagtttc
     2221 ttttctctaa tctctttatc taaaagaatt ttgatattga cttcatggtc agagcagtta
     2281 acgccggcgg aattatcaat cgcatcagtg ttaataagac cgccctttaa tgcaaactcg
     2341 acgcgtccaa gttgagtaaa acccagattt cctccttctc ccactacctt gcagtgtaat
     2401 tctgcgccat taatacggca gaattcatta gtcttgtcgc caacatcggc atgtgactcc
     2461 gtggttgctt tgacataggt tccaataccg ccgttaaaca gtaaatccac tggtgctttt
     2521 aacagcgctc gaataagctc attgggcgta agggcatttg catctatagc gagagctttt
     2581 tttacttgcg ggctaatggg gatcgatttg cttgagcgtt tgtaaacgcc tccgccttta
     2641 gaaataagtt gtaagttgta atcttcccag gatgaagtag gtaaattaaa caggcgaagt
     2701 ctttcttcat aggattttac agggtcggga ttcgggtcga gaaaaatatg acgatgatca
     2761 aaagcagcta acagccgtat atgttttgaa taaagcatgc cattgccaaa tacatcacca
     2821 ctcatatcac caatacctac taccgtaaag tcttgttgct gaatattgat atcaagttca
     2881 cgaaaatgtc ttttgacaga ttcccacgca ccacgtgctg taatacccat ttttttatga
     2941 tcgtacccgg cagaacctcc agaagcaaaa gcatcaccta gccaaaatcc atgttccttg
     3001 gcaatactat tggcaatatc ggaaaaagta gctgttccct tgtcagcagc aacaaccaga
     3061 taagggtcag ggtcgtcgta acatataaca ttctgtggag ggattgtttt atcattgatt
     3121 aaattatcgg tgagatccag taaaccattg atgaacgatt tataacaaga gatgacttcc
     3181 tgttggattt gctcgcggct ggctgcatga agcggttttt tcaagacaaa acctcccttg
     3241 gcgccagagg gaacaatgac tgcgtttttt accttctgcg ctttcataag tcctaaaatc
     3301 tcggttcgaa aatcttctgg ccgatcggac cagcgaatac cgccgcgggc tactttggta
     3361 ctacgtaaat ggattccttc aaaacgtgtt gagtacacaa aaatttcata aagcggttga
     3421 cctggaggta ggtcaggaat ttccgaggat tttaatttga aggaaaggta atctttagat
     3481 aatcccgcgt gatttttttg aaagtaatta gtacgaagag ttgctttcat caaagaccaa
     3541 aaataacgca tgatgcgatc ctcatccagg ctgctaatcg catcaatgtt tatttgaatt
     3601 ttctgttcta gtgccaatat gtccttgtga atagcagatt tccttttaag gccaaatttc
     3661 aaataaaaga agtgaatcag ctcacgggca atggccgcat gcatttcgat ggttttttca
     3721 atgtaagatt gactgtaacg aataccaatc tgatggaggt attttgcata ggctcgcaaa
     3781 atagttattt cttgccagga gagccccgcg cataatacca atttattaaa accatcgttt
     3841 tcacaaatac caagattgat actaatcagt gcctctgcaa aaatatcttt gacctgagta
     3901 atgttgattg aatcggtttg agaataggtt acgttaaaat cactaatcca gaataattgc
     3961 ccgtcccgtg ttttgatttt ataaggacgt tcggtataag tgcgcagtcc catgttttca
     4021 agcataggca agatgtcgga tagggggatt ggggtttcat accgataaag tttaatgtgc
     4081 aaaggatatt gtgagtgagt gttttcataa aaatcgataa ccaggggatt ttctttggag
     4141 agtttttcta tctgtaaaat gtcctggatt gcctcttcgg gagtatgttg ttcactataa
     4201 ctcattgaca ttgtttcggt gtatttatca atgagctgtt tcccttttgg ggagccaaat
     4261 tgctgagtta attgcttttg caatttatct ttccatgagt ttgtcatcat tcttaaaatt
     4321 ccatgcaaga tatgttttta tcgttactca aaagtatagg ccatttcaaa gaataaaatt
     4381 aaaatttact aacttatttt acacaaagtg agtcatttgt tttcaggaaa ttattaagta
     4441 aactttaata aaaggttaag gacataaaag tataataaag cttctttcaa aagacgaagc
     4501 acctgttatg tataaaaata tactgttttt ttcaaatttt agccgtatgc gttggaacga
     4561 gtacgacggg gatcgatatc aaagtctttg ggaacataat aagcgagata ttaaacacac
     4621 atttttcggc acgattgcaa acggaaaaga tttgagagaa gtgctattcc agccagttac
     4681 tgcttctttt tcgatgctag tcgcgggggc atttgcccaa ttactggtgc cctattccat
     4741 tttcatgctc tgtatgggtg aggcggggtt tgcgggcaga acattaatta attatcttga
     4801 aataactact gttgctttgg caatgccatt tattgcttta ttaagtactg taggccgatg
     4861 tattgcaacg gttaaatgcc tggttgagga tcaatacaat ttggctgaga tggttgaaga
     4921 caaagaggtt aagccaaata aaatctatct tgattgtgct gaagtaaact acactttcag
     4981 ggatgagaac aacaatcagt tcagccgcaa atattataaa ttccaatata aaatgagagg
     5041 tctcaacgat gagctttgcc agggaagctt tactttcaga gatttagagc gtgctcctga
     5101 taatgacgat ttcaatggaa agctttccag tctcacactt tataaatacg acatcctgga
     5161 tttgattaaa agagttcata aagataaatt tgaagaaatg gaagacgaaa atctaattcg
     5221 tatgtccatt taatttagat tagtttaaaa atcaggatgt ttttgttgaa agctataatg
     5281 gatagtgatt atttttctca actacttgtt acttaacagg ccgcgttata tcgtttatga
     5341 aggatgttat tggttcccgc ttatattttt gatgtgctga cttaataatc agttattgag
     5401 tcggtattta aagcaagaca aaacagagtg atggactaaa taaaattatt aagggaatga
     5461 gagcgtgcgt gtattttatg gcttattagg tttcatgata tttaatttgg tggcttgtga
     5521 aaaaatggca ttactgacta cgccacctaa aaaacagcaa ttatctaata gtccactggc
     5581 tgctcgggca gagcgatatt tttggcgaac ccttcatgag gggcgatatc aggacattcc
     5641 gcaagcggat tatttgctga tggcagctta cctcgagaat ccatatgatt cgaaattagc
     5701 ggcccatctt ggctttattc atatttggaa aattactgag cgggaaagag taaaaaataa
     5761 ctctcccttg cttcccaatg aaattatttt gtctaaaaaa tactttgctg atgctctgca
     5821 actggatccg gaaaacccaa tttatcaagg attttatggc gatacccaat tagtagaagg
     5881 gcagattttt aaagacaaac aagaagaagt cagaggatat tttactttac agaaagcgat
     5941 aagcaattgg cctgagttta attattttac tgcagggtac cccatgagta gtttaagtgc
     6001 tgattcggat cattttaaaa agggtttgga ttggcaatgg aaaactttgg atatctgtgc
     6061 tggtgagaag attagtagac agaatcctga ttttacgcct tatatgaaga gagaaacgac
     6121 aataggcgcg caaagagcct gttggaattc agaaattgcg cctcataatt ttgaaggctt
     6181 ttttatgaat atgggggata tgttagttaa atccggagat cctgaaacgg gaataaaaat
     6241 atacaataat gccaaattga ctaaaaatta caataaatgg ccgtataaag acatgttgga
     6301 gaaacgtatt ctgaatgcga aagccaatgt tcaacatttt aatcaaaaat caaatgaccc
     6361 caatcagtcg attatgttta attccggata tggatgcgtg gtttgtcatc aacggtaagt
     6421 accatctgat tattctatat caggtggctt attagattag taagaatgtg aggtttcaat
     6481 ttaaaccccc tgcttgtgtt tttgcaaagc gggcaggata aaagcgaagg aaagtgttat
     6541 agttttaatt tatttcggca aagaatttaa aaagaacatg gaaaaaatta ctatttatca
     6601 taatcctcga tgctcaaaat ctagacaagc attggaaata ctgcaaaaca aaggttttga
     6661 acccattatc attgaatatc ttaaaactcc tcttgatctg gaacaattga aaagattacg
     6721 gactcaccta gctcttgaag acttagtgcg cacgaatgaa aatgttttta aggaattggg
     6781 tctgtctctg gctaatgagg cgcaaatttt agaagctatg gtaaatgaac ctatattaat
     6841 gcaacgccca attgtcacat ttaaaggtaa ggctgttatc gggcgtccgc ctgagaaagt
     6901 cctggagctg tttgattaat ctttattgta cgataatata aatttaatcc tgataccatg
     6961 actaatctca ctgtcatggt aaggaataaa agcatttgca aaacttcaga gagaatagct
     7021 ttttaattgt gatgcaaatt gccttaacac atgcagcccg gattcttcgg cctggctaca
     7081 ccatttttgt attaaattaa gaagatcctt attggatagg gacgttttta accatatctg
     7141 ctgtaataat tcgcggtaat tatgaaccgc ttgcaagttg tcatgttgct taagtaatgt
     7201 tttcaatcgc aactgatcag catgggatag ctgttcgttc ggtttcttga ataaatggcg
     7261 agcattcttt aaatcccgat tttttatgcg gtattgcata tcgcgcagag taggaaatat
     7321 aaccagcttg taataatcag atatcacttg aaagcgatta ccaagtatcg cttttaccga
     7381 atctacatct atatgagatt tattcttgtc aatgatcagt tttgggggta atttttttac
     7441 tttggcaagc tttaaaaaag acaaacaacg tatgtaaaac caacccaaat caaattccca
     7501 ccatttaatg gaaaatttgg ctgaggaggc gaaggtatga tgattattat gcaattcttc
     7561 accagcaatc aataaacccc agggaaaaac attggttgaa gaatctgacg actcatgatt
     7621 gcgataaccc caatagtgcc ctattccatt gaccacgcca gcaacaagag gtatccacat
     7681 catttggatt gcccaaaggg taagtccagg aattccaaac aagaaaagat taagtgtgag
     7741 cattaaaaat atgcctttgt tcgcatgggg agaatataat ttgcgctcta tccagtcgtt
     7801 tggagtgccc gaggagtatt ttttaatcat gtctttatct ttagaggcct ctcgatataa
     7861 ttctggaact tgccaaaata ctttttttaa agtaaatacc tgagggctat gtggatcccc
     7921 ttccatgtca gtattggcat gatgtttccg atgaatggct acccattctg ccgtggtcat
     7981 cccggtcgtt aaccaaagcc agaagcggaa aaaatggctt acaagaggat gcagagttaa
     8041 tgcgcggtgt gtttgataac gatgtaagta aatggtaaca gcagtggtgg tgatgtgagt
     8101 caggacaaaa ccggccaaaa gatatcccca taaagtcata tttaaaaaac caaacatcat
     8161 tcctcctctt aggtgcctga aattgtgtaa ttggcaaaac gatatgtaag aatatcttta
     8221 tatccactaa gtatacacta tttattttta tcttagtggc atttaaaaag ctaattattt
     8281 acgcgctttt attaaaagcc gtcctggatt ttctgctgat attgccctta tatcctgctt
     8341 ttggcggcgc aaatttttga attttggctg agacaaaatc tgaaaagtta tgcaaaaact
     8401 caaaatcttt aattttaatg cgctccagtt tttgattgat taatttttct atagacagca
     8461 gcagctttat ttcttctgtg ctgaccaagg atacagccaa tccagaggcg cctgcccgtc
     8521 ctgttcgtcc aatccgatga acgtagtcct caggcacatg aggcaggtca aaattcacta
     8581 cacaggcaag tttctcgatg tctatgccac gtgctgcgat gtctgttgca attaatgttt
     8641 gtactttgcc tgacttgaaa tcagctaagg cttttattcg ttgagcctgt gatttgttgc
     8701 catgaatagc aaccgaatat atttgggatt cggccagttg cttaaccagt ttattggctc
     8761 catgtttggt tcgagaaaaa accaaagttt gtccccagtt attcttatga agtaaatgac
     8821 tcagaagggc taacttatgg tttctatcta ctgaataaac agtttgtttt atggttttta
     8881 ccgcggtatt tctcggtgcg atatcaattt caaccgcttt attcagtata tttctggcta
     8941 aagcgcgtac ttcaggggta aaggttgctg aaaaaagcag gttctgcctt tttaaaggta
     9001 ataatttgat aatttttttt atatcatgaa taaaccccat atccagcatt ctatccgcct
     9061 catcgagtac cagggtgtca atctcatcaa atttaacagc acgttgctga tataaatcca
     9121 ataatcttcc cggtgttgca accagtatct caaggcctga gtctaattgt ttaatttggt
     9181 tgtggctttt tactcctccg taaatcacag ctgagcgcag tgttaggtat ttgccatatt
     9241 ggattatgct ctcgtggacc tggatggcta attcacgtgt aggcgttaaa atcagtactt
     9301 taactcgatt agggctggtt tgagtttgct gactggcttt ttgcaagata ggcaatacaa
     9361 agcttgctgt ttttcctgtt cctgtttgag cggaaccaag taaatcatgc ccatttaaaa
     9421 tgagcgggat ggcttttaat tgaatactgg taggggtagt gtagcctaac tcactaaccg
     9481 ccctgtttag cggctcaatc agcgctaatt gtttaaaact cattgttttc cttattgata
     9541 tctatgggta ttgtgggctc tgaggttaat cactcattat tttgtgggga aaatgaggca
     9601 ttaatttaga ggggaaaaat aggaattttc aaaggttttt tgaaagaatt catgcaatat
     9661 ttggaactat tataacagtt atttgcaaat atgtcttgtt tattttgctt aggcttggat
     9721 tgttaattaa cagctaacat gctaagctcc tcagaagatc taattctacg cacaatactc
     9781 ttttcgatcg tttgattttt tactcttctt gctttcatcc agatcattgt tcgttaatct
     9841 attcaggagc aatgatgaaa cagaaaaaaa tatatgttgg taatttacca tttggtacga
     9901 ctgaggaagc attaagcatt caattttctc aatatggaaa aatcgaggag atgttcttaa
     9961 ttaaggatcg attaacagga caaatgaagg gatttggttt tataacgttt tctgctcagc
    10021 aggaggcgga gtctgcttta gagatgaatg gtcagccatt tgatggacgt tcattaaaag
    10081 tatgcatggc acagaaaaaa accaattctg tttctcgtag ccggtggtaa aatagagtat
    10141 ttacttaatt ccgatgttat cgtatcgaga actcatcact acaaataaat ttttaatcta
    10201 cgttgactca acttttttag ccctgagtga gccatttatt ttcttgtgtt ttatctatcc
    10261 catatgcaaa aataacaacc atagtatttg gtgataatag tcagcatgaa agcgaacggt
    10321 ttttttgatg agcaatcttc ccttaagaaa agtaaaattg ggttgaattt agtatggatg
    10381 ttctcacttg gtcatatttt tctaataacg ctcagtaatg ttctggttca atatccattt
    10441 gatatgatgg gatttcacac gacctggggg gcgttcactt atccagcaat ttttatttta
    10501 actgatttga caacaaggat agcaaccgct aataaggcgc ggaaaattat tatgctgagt
    10561 atgaccccag gattaatatt gtcctatctt gttgcgagct atattgaggc aggcaataat
    10621 ttgaatgaga gcggtctatc cgttttacac ctcatgccat tacgtatcgc cctggcttgt
    10681 tttatagcct acgttgttgg gcaattgctg gatgtggcag ttttccaacg ctatagaaat
    10741 cggcctgcct ggtggctggc cccaactcta tcggcggttg caggcaattt catagataca
    10801 gcattatttt ttaccatcgc gttttatcac tgctccaatc catttttaag tcagaattgg
    10861 cctgaaattg cgatggtcga tgtctttttt aaaatcatga ttagtttaat tgcttttgtt
    10921 ccggtttatg gtctggtttt aagtattctt ggtataaaaa ctaccaataa attaatggta
    10981 taaaggtatc ttgataccat tttcaaaata aggattaatg atgctgactg gtttaaggtt
    11041 gcttacaaaa gacctcaaaa ttttaaatgg caaatacatt cagctagaac ccattaccag
    11101 ccagcaccgt gaggagttac gaaaagcagc aaatcatgaa caaatttggc aatacatgcc
    11161 gcaaaaggca acgggcaagt tatttaattc ctggtttgat gattgtcttg agaaaatgtc
    11221 cataagagac caaactactt atgcagtgcg ttgtaaaaaa gaaggagatc tgaaaggagc
    11281 cacttcattt tacgatattc aaccagaaaa caaacggctg gctttaggct atagttggta
    11341 cattcctaaa gtatggggaa caacgacaaa cccggaatca aaattactca tgttgcatca
    11401 ggcgtttgat gcctggggga ttaatcgagt agaactaggt acagattctc gcaatattca
    11461 ttcctatcgt gctatcaaaa agctgggtgc gacggaggaa ggtgtgttgc gccaacatat
    11521 gattcttcat aatcaggtga taacggatac tattatgttt agtattcttt tctcggagtg
    11581 gccggcggta aaagagcgat taatacagag attgaactat gctgcaaccg ataaagacca
    11641 cttggctgtc tctatatagt aatcccttgt tctctgggtt acacagctca actcatgaaa
    11701 taatcagaca cacaataaat agaatgaaaa aagattggta tttttcttta attgagtgaa
    11761 tatttggttc tacctgcgag aaaggagtgc tcaatgtaca acaaaatgtt taagcctctg
    11821 gatactgatc cgattttata tttcaaaatg tacagtaatt atactgaggg aagggttgat
    11881 gattgttgtg catttatttt aatgcccagt gggttacaaa gagattgggt ctgtttgcaa
    11941 tcgattcaat ttgcttttaa taagtgtggt gacgtccttg ggattaacat tattttttct
    12001 ggaaatgagt cgaatatcca caaaaaggtt cgagaaacga tggaagggat gctcaagctg
    12061 aagcttcaat atggaagagg ggaagagctt ttcgtttttg atgaagaaaa gaaaacattt
    12121 catctgggga tactgcctgg taaggataca caggcgtatt tggagggtat tatcgctttt
    12181 attcaggata gttatcgctt gcaaccggac tttgcccaaa acatcaaggc acaacttctt
    12241 aataaggaat atctggcaca ggaatatagt cgtttgcgat ggaagccacc agagaaagaa
    12301 tctgtttgtg tgctcatgta ggttgtagaa gcattgtttc cactttaaaa atagaaaaat
    12361 ttgaatttta tggggattat tgcattccat tagcttggtt tgcgtaatac tctcttgaca
    12421 actacaagat tgaaagctca atattcttta tttacaccaa ggttcaggat tttcattgta
    12481 taggaagcga tggctcattt tattgtgtgc tttgttgaat aatggttgcc tctggcttgg
    12541 gtcagaatac aggaaacctc atgtcaacac cccgcaaaaa tggccgcata aatattcaat
    12601 acaaactgag caagctgcct atttacctga tctgcattgg tggaaacagt tcaatagcaa
    12661 ggagctcaat gcttctatcc aaaaggcatt acaaaataat caccagattc acctcgctat
    12721 ggcaaatatt gaatccgcac aggggcaatt gcagcaagtc cagttaagtt ggttgcctaa
    12781 tctcacaggg cttgcaggtt acacccagtt tcccgtttta ggtaatccgg ggactacggc
    12841 aattgcctat ccagcctaca tcattaatat tttccagcaa tataagcaac aaaaaagcgc
    12901 gcaagccatg cttgaagcca gtatttacgc gcaatattct gccagactgg ttgtgatagc
    12961 ccagactgca gccagctttt ttaccttgat tgctcagaat gaagctttgc atttatacaa
    13021 taaactgctt aaggattatc gaacctatct caaactgact caaagccaat atcgctcagg
    13081 attaatttcg cttgacaata tcactcaaat caaaagtcgt atccagcgaa ttgaggcaca
    13141 aatcaaggtg gttcagcata acattgtggt gactaaaaac gccttgcatt ttttatttaa
    13201 tgaaaatcct ggagatgtcg agattaaaac tctctttgaa aatatcgata gtaatcaatt
    13261 ggtaccaggg aatttaccgg ccagcgttct cagtgtgaga cctgacattc atgaggcaga
    13321 agcgttgtta aaagcagcgc atgccgatgt gggtgcgata accgcgaatc ttttaccagg
    13381 gattaatttg ggcgcttacc tgggtgaggg atccaatgtg gatggcgcca ttaaattagg
    13441 gcaagcctat ttaaatggcc cgataattga tttgcctcta tttgctcagg ttgatgtgag
    13501 taaagctcga tataaggcaa tctatatcaa atatattacc actatccgta aggccttgcg
    13561 cgatgttgct aatgacttgt ctgcctattc aacttctagc cagcaattaa ataataattt
    13621 atcggcttta agcgatgaaa aacagcaatg ccatttggca gaagttcgtt atcgtcacgg
    13681 aattgatgac tatttaaatt taataaaatg ccaaatcctg ttagacgaat ttaagttagt
    13741 gataaaccag aacaagctgg aaaaactgct atccatagtc actttatacc aggatttagg
    13801 aggtggttcc cgtgggcatt gatcagcaaa agaccgtccg taccttgcgc ttatccctga
    13861 cgtgtatttt tctttttcta attacctggt actaccaagt cccagaaagc gcatggactt
    13921 tagtgaccat atggtttgtc atgtatgaat attccaccgt tggtggcgta ctgacaaaaa
    13981 gctttttgcg cttcgcaggc acggctttga gtgccattta tggaatgata gttgtttatt
    14041 tttgtgccaa caacccgttg attaacatta tggctttagt acctggtttg tttctttacg
    14101 cctatttttt catgggggga gataaaacgt atattggtac cataggggct gtaacgctta
    14161 ctattgtatt gctgaattat aacgacatag atacggctgt attgcgggta ttcaatgtca
    14221 ttatcggtgt cattggttct atgttcatga tccggttttt ttacccccaa tatgcaagag
    14281 ataaagtttt agagattcag ttgaacttta ttactcaact tgcaaatctt ttagaaagct
    14341 atttgaatcc aacccagtct ttggcagctg tccagacaaa gtatttggat tatgaacgca
    14401 agatgcttga tggttttact ttatacaatc gttatattgg cgaagccaaa atagaaacca
    14461 agaaagctcc gtttttcatc ccccattcca ttgctgccat gcaacatttc aggcgtctgt
    14521 ttcgtttgtt cagtgtgttt atttattatt tatctactga ggaaattcga tcggatccat
    14581 gggtatgtga tcaattaagt aaacttctgc gtaatttaca gtcactgcaa cgaaaattgc
    14641 tgcaacagga tgcggggccc atagggggag acgttgaggt tgcagagcca gaagaagaaa
    14701 tcgttataga aacacctaat attgaaaata aaatagcaac agaagctatt gtcaataata
    14761 tgcagaaaga aatcgctttg cttgatactg aaattaagga aataatatta atttacgatg
    14821 tttatgatat caggttgaat gagatgtaaa gactcattcc aatatcttgg gaagggttca
    14881 atagaaatga ggatgaaaaa gacacatgag tgacaaaccc aaagctggat ttcctgtaat
    14941 tattttatta tgcgtgtttc tattcattct gatttatcat tactcgtatt tatttccttt
    15001 tacaaacaat gcatttgttg ttgcgaatgt tcgtccagtt gctgccaatg taaaaggtta
    15061 tatcaccaat atttatgtta aaaatgaaca ggaagtaaaa aaaggccaac cattatttac
    15121 cgtattcaaa gccccctatg aattggccta caggaaagct gtgagccatg ttcaagaggc
    15181 gaaagcccaa ttattgttat tcaataaaca agttgagaaa acgaaatatc tgctgcaagc
    15241 acaaaaagag ctttatgaaa aattccgttt tgattatgaa cataattatt ctgcctcgcg
    15301 cgatcacgca gtttctaaac tgaccgtcaa tacgatgtta aaagaaaaaa atgcggcact
    15361 aagcaaatta aaagcattgg aaaaagagtt ggaggtcaac cagcaacaag tcattgtgca
    15421 aaaaaagaaa attgactctt taattgcggt gatgaagaat gccaaaatcg atttggatga
    15481 aaccacagtt tatgccaaac aaaatggggc agttcaggat atgtttgttg cattgggaac
    15541 ccccatcaaa acccgaaagc ctgtctttgc catagcggat accgatacct tgtttatcca
    15601 ggcaaatttc aacgaaacgg atttaagaag agtacagcca ggggataaag tttctatttt
    15661 cccgcgaatg tattttggat ctaaaattta tcatggagtg attctttcaa gaaactgggc
    15721 tgcaagccgc ctggaaaccc atcgggcaac gcaaatacaa attgttcgca atagcgaaag
    15781 caactggttt ttattgcctc agcgcctgcc agtacaaatt caaataaccg actatgaccc
    15841 ggtgcattac ccgttaagta taggcgctag cgcctatgtg tatatccata ctcattaaaa
    15901 ttatggaagg tgttgaaatc aagagtgcaa tatattctgt tttttttcta tttccagtat
    15961 taatttttag ctgataattt gactgtgacc attttttaga ctagctttag cttaataagc
    16021 tgaacaaaaa ttgatcaaaa tgtgaatttc atagtttaat gacaaggagg tcatatgaaa
    16081 attccatttg aagggaaatg gcgtgcgacc attaccaaac gtgaggctgg ttttagtcaa
    16141 cgtgtcgtgg ttggaagcgc aaccagcggg aatggcagtt acaatggtgt tgttggcaac
    16201 agctttgttt tcgaagacgg ttgggttgaa cttcagtgga ataacaacgc aggttcaggc
    16261 tggcaggaaa gcgctgtcat tagttctgtg ggaatgacga gtccgctggt tgttgtaaaa
    16321 tttgtttcag ccgatgacaa ttttccaaat caacgagacg gcgattatga tgatttgcaa
    16381 gtccgttttg aacatcttga tgctcctttt gaagtaacac aaagacctta cgctcttgat
    16441 aggggctccc ttgccatgtt tcctgacggc atttttgatg cgagccaggg gattcaatac
    16501 atgggagtac gaattaaaaa cacctggttt tttgattggc aaagtaattt tccggcaaca
    16561 ggaatgaaaa ttggtattgc gccagtgagt cgttccgcct tgtctgctgc tggtattatg
    16621 gtgattgata gttggacgcc acaggaacaa gaaggtttgg ggcaaatcat ggatggtggc
    16681 tttgtgagag ttcctgatat aaaaattaat gaggaaacaa caatttactt caagctggat
    16741 gtcagtaatg ccaatccctc caaacctgaa atcggttttg tggcacagcg cgatgcttat
    16801 gatccacgct atgatgaacc aacacgaata gttgataaaa aaattttcat cagtcgctcc
    16861 agttacaatc ctgcaaccag ggaattggtg actgcgatcc ctgaaggtac tttgtacctg
    16921 aaacttaata aaattgccat tgatcgcaaa ggcgctacaa aagcagctca ggaattgaag
    16981 aaatgcctta aacagcatgg aggtggcata aaagggatgt caaaatggct gcataagcta
    17041 agccgtggtt tttggcaaaa tagtaaagac aaggaacgtt ggtgcaagga gcaaggcaat
    17101 gatgcattac gtaagctttt aaaacagctt ttagatggaa aggacattga tatttgtcat
    17161 cttaaggcta ttttggatca atgttgtgaa cgcccaggcg gagattgcga gtgttgtcct
    17221 tgtggtgatg gagatggcag gccgggtggt tgggatgatg gtaccggggt tgatggctgg
    17281 tgccgagtga aacctgtatt ctggttaccg ctggattttg aatataaggt agtccctaac
    17341 ccggcttaca ttggccagtt tggtcctctt gctttcgagg atccctggtg gaaagtagtt
    17401 ttaatcatct tggcgattat actggctatc gcttcagtca tttatgatta tgtttatgca
    17461 tcagaagatg accaatacat tattgggcag gttgtccgta atggggatgc tccaatcaat
    17521 ctggttgatg cggcagtgag taatcttaat ggctctcgtg gagtggatct gggacaactg
    17581 gatgcccagg gtaatgatat taacaacggc agccccatta ctgtattaga cagtattatt
    17641 cagctcgatc gtacagcagc cggaagttat tttggtacgc ttgacgctgt tttgggtaat
    17701 gtcgtctgga agtcaggggc taccagtgca acaacgagag gatttatcag tactctggga
    17761 acttccgtca gcatacccta tgacgagcat gatacgcttt cagataccgt tacttacatt
    17821 aacgaagtgg ttatcagcga acttgccgcg attccgcaaa cactctcaga tcctggtgac
    17881 tcgggatccg tatgggttga tctggcaaca gcgcgtccag ttgctttaaa ttacggcagc
    17941 ccaacagact ccagtgataa tcgggctttt gctaatccga tacgctctgt tattgacgtg
    18001 cttaacttac ggttttaatg aaaaggaagt aagaaaatga aaagggaatt ttttcaaaaa
    18061 ctcgacaaga ttcttgatat tgcttccgaa cataagcaaa tgttgttgca atatcctgat
    18121 gtggtcatgg tgggtgtggg acctgagcgt cgcaagggaa aaattacagg ggagcctgcg
    18181 atcattgtta cggtacgtaa aaagcgctca tccaatgaat taaagaaacg gggagaaaag
    18241 cccttacctc agacaattaa gggaattcct gttgatgtga ttgagcttgg caaagccgtg
    18301 gaagctcccg aaattatcgc tgttcaggaa aaagcaaaaa aaacaattag taaaattcgg
    18361 gcaaaatggt tacaaacacc aaatattaca gcaataggat atggctacaa agagaaagga
    18421 ggggaaatcg attttaacac cattgccatc cagttttttg tcaaaaaaaa atttcctatc
    18481 gccgagctgc aaaaccagca aataaccccc attcctgaaa aaattaatgg ggtgagtact
    18541 gacgttattc agttatctcc catccgcccg gctgcggctt ccggttccag aggagatcgc
    18601 catgatccgc tcaaaggagg tgttgcgatt ggagtaggaa gcaagccatt tcattacggc
    18661 acttatggtg ccacagtatt tgatagaacc actggcgaac gcctctttct tagcaatcaa
    18721 catgttcttg atgcctctcc tggaacagac accatacagc cttcgccagt cttattggat
    18781 gattcagtgg aagtaggatt tcaattggat ttttgcttgc ctatccattt tatcaggctg
    18841 gatactccaa atactactgt tggcactgtg ctggctgggg ctgccgtagc tgcagctgtt
    18901 gccgctgctt taagtgatga aattgatccg acaaggcgag gtcaggaagc aacaatgcct
    18961 gcgacaggat ctaaaacatt atttgaaacc caaaaagtta aaatggatta tcctgaattg
    19021 cccattccag gaacaccgtt taaagtaaag acagcttggg attatgaacg ccacaccgat
    19081 gcgggtatta agacattcag tgtcagtgaa gaaaaaaaga atccccatgt tttacgcgat
    19141 aaattattaa ttacagacaa aaaactttat catcctgggg atactattcg tatttttggt
    19201 ttaatcttgc tggaggaatg cgcacctgca tctcagacca gggacgatcg tcaacccatt
    19261 agtccccggg aattggaaga tataacccat tatcctaatt tggcttcaag tatcaatctt
    19321 gatctggtag ccacgatgga ttctgacatt gcctcttcgc cagcgcgtcg tacgaataca
    19381 agggctgctg tcattaaccc caaacggtgc cgttgtgatc gctatcaccc tgtcgccatt
    19441 ctgacaccaa cgacggagga tcgtgctttt ccagttgttt tgcgtgaacc agtctttgcg
    19501 caaaaaaacc agatttttca agatttgcta accgtagtga agcaacgaga tgatgaaaag
    19561 ctgctggagc gggtgtttgt tctggttcgt tatggttgta tatataccgg cacattacgg
    19621 gtaaataaca ttccagctgg tccatggaaa cattttttct atgttcaaac agtgaacatc
    19681 gcaacaagtg atatgaagcc gctgctggcg gcgcaaatta ttggcggttt acctgtttcc
    19741 gaaaatgcta aagcggacct ggatatagcc tgtggcccac tcacctggga agatggtgag
    19801 tttgatattg agttgatata gagcgtgctg cagggctgat tcatattggc caatagccat
    19861 ggaataatat gagttgtatt gataagtaat accttaaaat caaattattt tcataagcta
    19921 taaaatagtg ctatgctttt gccgccaaaa tggtacgaag actattaagt cacattagat
    19981 acgttttatt ttgaaaggaa tactctatgt ttggtttttt tagtgattac aaacaataca
    20041 tcactctgag aaattttgct gtcgtttata acgggcttac aggcttggca gtattgtata
    20101 gcttgtggag taatcctgag gctgatccct ccgaatacgc cattgatatc tctattcacg
    20161 cgctgactgc tatcacttta atgtgtaaac aagcccccga atcagttaaa gcagttgcta
    20221 tggcattaaa tacttatagg ggatttgatg ccttattcaa ggctatcacc tcacttcctt
    20281 ctacaatccc aggtatcgcc aatgctgttg atgtccttaa tcaccgtttt aatttcaagg
    20341 agcttgaaaa acttggtaat gaggaaactg tagaaaccag atcagcagtt cagcatacga
    20401 tgtaaagtct ggatcgtctt ttccggtaga agatggattt atcagccatt ttctatcggg
    20461 ttctcttaca ccaatttcat gctaaatagg gtgtgttgac aattggctcc caacgcctac
    20521 gtgccgcgac ttgttcgcgg catccatgag ctttaatcag ctgcagattt cttcataaag
    20581 atcgcgccat tctggattta actcttcaat taaatttaac ttccactgtc tgcaccaatt
    20641 tttgaaacgt ttctcacgac gagcagcttc tatgtacgtt tcatgcactt cataatagac
    20701 caagatatgt acattatacc gtgctgaaaa ccctggaatc actttatttt tgtgttccca
    20761 gacccgctta attcaatcag atgttgagcc aacatatagg gtgccattgc gcttacttgc
    20821 cataatatag gcataaaatg atttcatttt tcttccttgt tatcgagttt tggactcggt
    20881 tcgataatat cattcaaatt atgtgggtta cataggaagc taaatatatg agcatgccat
    20941 tgctagcgtt gaagcataat tccgttttaa cttatcgtac cttgttgcaa tagcacgaaa
    21001 ctgtttaagt ctgacaaata tattttcaac taggtgacga tatttatata aaccccaatc
    21061 aacttcagct attcgaagga ccgatttcaa attgaccatc tcctggatgc cgcgaacaag
    21121 tcgcggcacg taggcgttgg gagccaattg tcaacacgcc ctagtttatt ggccgtaatc
    21181 caacagaaaa tttttcacag ctaaccaatc aatataagct ttctttttat ccgagggatt
    21241 gcgcaaaaga taggcagggt gatagcttac gataaaagga gtattgtcat agtagtgaat
    21301 atgattacgc agctgcttca gaggaagtgg tttatcaagt aagaattgtc cggcgaaacg
    21361 tcctaacgca aggattaagt gtggtttaat gagttgaatt tggcgagtca ggaatgaact
    21421 gcattgagaa atttcctcaa gggctggatc acgattatta ggggggcgac atttcaaaac
    21481 attggcaatg tagatatcat tctctgtcat ttcaatacta cgtatcatct tgtttaataa
    21541 aagcccagcc tttcccacaa aaggttgtcc tttctgatcc tcataaaaac caggcgcttc
    21601 accaataatc atcagttttg cttttggatt cccacgagaa aaaacagttt gggtgcgtgt
    21661 tttatgaaga gaacaacgta cacaggatgc cacttcatcc gctaaagccg ctaaatgctt
    21721 ttcacaagaa ggagtggact cacgcatcac ccagacttca attcccatag tctgtagata
    21781 gtaattgttc aatacatctg acataaaaaa ctcatgatat taataaaatc cggattatag
    21841 attagactta ccttttgtag cttggtactt acgcacctcc aatctctttg ttaaagacat
    21901 tatttgcctt ggccttggag ttttgcttaa gtcctatagt taatctattg tatcagtcct
    21961 attttaaaga aagaaactta aaaaaacatc atatactcac tgcctgtaag aaatagagag
    22021 ttgagggggt gaagaatatt aaacagagac tggaaagaat atgaatagcc aaaaggattt
    22081 gaaacaatta gatattaagc aagtgattgc ctgggcattc tacgattttg ccaattcttc
    22141 ctattttgtg gtgatattta cgtttgtctt tgcaacctat ttcacaagtt acattgcccc
    22201 caatactatc ttgggaaccg aattgtgggg gtatacgatt tcagtttctg cgttactcat
    22261 tgctctggtg agtcctatcg ttggtgcaat agctgatttt agtgggcatc ataaatcctg
    22321 gctattcgtt tttacctatt tgggcgttat ttcaacaggt tgcttgtggt ttgcctatcc
    22381 tgacagccat tctattccct tggttttaac ttgtattttg attagcaatt ttgcattgga
    22441 agtaggaact gtattttata attccttttt ggcaaattta gccccggaaa actatctggg
    22501 gcggatttct ggctgggctt ggggttgtgg ttatctggga ggattgctgt gcctgattct
    22561 atccttattt gtttttgtga aaggtgaact tggtggatta tggtttggga cagagaattt
    22621 tgccaatatt cgtgctgtca ctgtatttgt ggctttatgg attagtttgt ttagtttgcc
    22681 tttgttttta atggtgcaac agaaaacagg agagcatttg acagtgggta ctgcgattag
    22741 gaaaggtctg caagaattgc ggttaacaat aaaaagcctg cctaaacaac gtgatctgtt
    22801 cttattttta atagccagaa ttttttatat cgatgggttg aattctatat tggcactagg
    22861 cggtatttat gcggctggca actttcactt gagtgttagc gatattatgg tgtttggaat
    22921 cattttaaat attactgcag gtcttggcgc cggattattc gcctggtttg atgattggat
    22981 tggatctaaa aaaactattc taattgcttt agcgtgcctg acactcactt tttgttttct
    23041 gttaaccatc cactcaaatg cgttattttg ggtttttgca ccggtgtttg gtatttttgt
    23101 tggtcctgtt caggctgcca gtcgtacttt cttggctcgt ttggcaaaac ccgaagaaat
    23161 gacgcgaatg tatggttttt actctttttc tggtaaggca accagttttt taggaccttt
    23221 cctggttagt acaaccactg ctttagctga cagtcagcgt ttggggatgg ctgttcttat
    23281 tccttttttt atcatcggag gcggactatt attattggtt catgaaaatg aataagaaaa
    23341 attccggtaa ggctgatttt ccctgttttg aggttcagta tgagtttgaa agacagggga
    23401 aaaattcgcc tctttgtttt tacaagttat ttcttatgat aaactttgtt ctaatatgaa
    23461 aattattata tctatattat ttgctttcat tagcgcctct gggcatgcgt tctcttgcta
    23521 tgatccacag actatcattc aaaaacccat acagtttgat aaaaaacgga ttgccttaac
    23581 ccgggaatat cagcttactc attatggtat tgattccgag tcgatagaaa ttgagcctaa
    23641 aatgattgta ttgcattgga cttgtatacc aagcctcgat actactttcc gcatatttga
    23701 tccccccgct ttacccagca gttcgccaag acgaaatgaa ttgcccgggg atttaaatgt
    23761 atcaagccat tttttagtcg atcgggatgg aactatttat caactgatgc cagaaacatg
    23821 gatggcaagg catgtgattg gcttgaatca ttacgcgatt ggtattgaga atattggcgg
    23881 tgttgatagt aaagacgatt tgactgagga gcaagccaag gcaaatgcct ttcttgtgtg
    23941 ctatcttaag aacaaatacc cgcaaataaa ataccttatt ggtcataatg agtatttgca
    24001 gttcaaggga acggctttgt ggttggagaa ggatccaaat tatcaaaccg ataaaacaga
    24061 tcctggtcca acgtttgcta aaaaagtaaa acaattagtc cttgcgtcat aagtagaagt
    24121 ataggcgctt atgcaggtaa tgtccgttgg gatttctgtt tagctgttaa ttgttatttt
    24181 tggattgcct tatagttttt aaataaaaat cgggttttgg gtagtgttgg tattcaaacg
    24241 ttgtaaacat tcctgcgttt aaatgatata aattatggca atggttagcc cagatgccag
    24301 ggtttagcgc atcaaactgt actttcactg tcgaatgcgg ttggactaac agggtatcgc
    24361 gcatggctcc atccaatggc tgaccatcaa tttcggttac ttgaaagaca tgcccatgga
    24421 aatgcatggg gtgagacata gaggatttat tttcatagat catttcaaca cgatcaccga
    24481 aatggattac ttttggcgag acatctggcc agctttgctt gttcatggtc caaatataac
    24541 cattcatctg cccgtcaagc acgtagtgca aacgagcatt tattttttta ttggttaaag
    24601 cattttttcc ttttagtttt ttttccaact catagtagtt gacacgtccc gttttattcg
    24661 tggtattggg agatagtatc ggggtaggtg tgttatctgt ttttagaatt agaccggttt
    24721 gtttgtttgt tccttctgcc atggctttaa tagggaatgc tccgcctgca gaaggaatct
    24781 caacaagcaa atccatacga ttaccaacgc caataggaaa agaactatct acaatcggct
    24841 caatgttttc accatcaaca gcaatcaaag tggctgatag tttcccggta tcaattttaa
    24901 aattggtgga ggaagacgca ttgatgattc gtaacctcac tttttttaag ggttgaactg
    24961 aaattacatc aggtgctgcg aatgtttttt tgttggcgag aaatgcatca taggtcacgt
    25021 cattcaaatc gttaccggaa tccattgcct tcatgtccat tttagcggtt cttaactgat
    25081 caaatattaa ctggggatcc ttgtaagtaa aatcctctaa aaacagaatg acttcttgta
    25141 agtctttatg cgggtcatcc ttgtcataaa taatcaaagg ggcagaaagt tgcttttgct
    25201 cttgaaattt ttgatgcgag tgcatccaat aggttccggc ttgcaataat ctgtagttaa
    25261 ataatttatt ttgccctggt tgaatcagga tttgactcac gtcaggtact ccatcctgat
    25321 cgcttgggac aattaatcca tgccaatgaa gagttattgg tgacgcactt ttattttcaa
    25381 ctaaaacatg aaacatatct cctttattgg caaagtaacc tttgttgtcc tttgtgccgt
    25441 ccgggtgggt aaaggtgagt ttaaattcct ttgttttttt tcctttacca tcgacctgga
    25501 attcctcttc actgacggtt atgaccgtcg gtttaagttt gctatctgtt gcataagaga
    25561 aattaaaaag ggttatcatg atagttattg ccatgataaa aacgaatatt ttacccaaag
    25621 tgcgcactgt aattttatta attagaattt tttgccaacg ggctttgtcc agaggagtta
    25681 ttgaataaaa atctgttgta ttggtcattt ttatgatctg tccatgtcca tgatatttat
    25741 aatcttttta tcagttaata gcacagtaag caaatctctg atttataggt tatcagaatt
    25801 agttttaagc atgaggtatg cgattgcaaa taattcttgt ttgtattatt gttctcattt
    25861 aattaaatga cgctgtattt tcaagttgaa ataattcata aaggacaagt gacttgatgt
    25921 ttttacaagg aaaataatga aaaggatttt tttaatatcg ataattggga tagttttgaa
    25981 ttctgcgtac gcttttgagc atcttggaga atgtggggct catacctggc atgaagtgac
    26041 atccaaatca ggttcctgga aaatcacttt gaaaatgatc agtcagaatg gccagtttgt
    26101 tgatccttcg tgtgcagaaa ttattgaaac aggattggct cagaataacc ctttgagtta
    26161 tggccttaaa ttcagcaaag acgctgattt taatctggcg tatactttaa cggctgtgag
    26221 tgaagaacag ggttttcaat caaaagcctg tgtgtttgtt atcactgcta atgggcctgc
    26281 gcaaccggat attcaggcgc ttagctatca tggggctgaa tgcttatggc gggtagttaa
    26341 aggagtaggg gaaaatttta gtgtcggtta gctctatgaa gtagcctaaa accacttctt
    26401 ttttctgaaa attaaaaaca gtagcagagc acacagcacg attaatccaa gtgcccaaaa
    26461 gtagccatat ttccaaccta attccggcat ccagtagaaa ttcatcccat aaattccggt
    26521 aattaaactt aaaggtaaga atatggctgc gaaggcagtt aatactctca tagtctcatt
    26581 catgttttgc atcaactgat tgtcgatttg tcctaataag ccatttaata tatcgcgaag
    26641 agagtctatt tcattgacaa taagatggca gtgattcgat aaattatata aggagtttcg
    26701 acactgctct gagatgacta aaatatttcg gctagtgaga cgcattaata tttctcttat
    26761 tgctatggta taacgcttaa ttttcattac accatgtttt aattccaaaa cttgcgaata
    26821 ggtattttta ttggtggtac gagcttgcgc gtccaattga tcggccaatt cttcataagc
    26881 taaatgaatc ttggcataat cattaattac tccttccaga agcaaaaata acaaaaaaca
    26941 agatgttttg gcatagggta aggattttgg gcagcttttt aacaggtcaa acaaaactga
    27001 agaaagtgtt ttagaagctg taaagcaata atttggtgtg agatgaataa tcaaattgcc
    27061 aaaatcagca tcataattgt cattcaactt cattgagcac aagccttgaa tttgcaaagt
    27121 gagcgcttca tcgatctcta tggttgtgga tctattgtct ttttctccac acaattgaat
    27181 cacttcttca ggtaatcgta atttttctcg caatttttta aagatgtttt tctggtttaa
    27241 atcgctatgt atccaataaa ttttatcttt atcctggtaa tcgatgttta aatcctcaat
    27301 cgcaattttt tgcatactat gattttttaa atcaaattca acagcgattg aatcaaacga
    27361 atgcatttca atgctcatcc ttattcgatt aatttcctca tatagtttaa gaataggaga
    27421 cttaaatgat attgacaaac tatttttaaa tagtccgggt tttccaatag actgtgagtg
    27481 tatcgtggag agttaatctc cagtttggct ttcgtcttgt ttatataatt cagactctgc
    27541 tttgggcaag gaaaagtgaa tttaagccta aatgagtata aaatcaaaat ccattaatat
    27601 aggttatttt tgagtgctta atgaatcaag atatggaaaa aattgaaaag atgtatcaac
    27661 tgattatgga aataaataag ttgaagcttg ttttccgtaa taccgtaact aatcctcaac
    27721 ggaatgaaag cacagcggag cattcctggt cagcctctat gataaccctt attctcatga
    27781 atcaattaaa aagagaattt acagaggtcg atgaattaaa aactatcaaa ttggttctta
    27841 tccatgatgt cgttgaaatt tatgctggag atgttctggc ttttgattta gcggcaagga
    27901 aggataagga aaaagttgaa ttagaagcgc ttgaaaaact aagtgctgtc tatccttcat
    27961 ttgggataga attggatagt ttatggcatg agtttgagga aagaaaaagt cttgaggcaa
    28021 aaatagccaa agcagccgat gcgatttgtc caattttcca acgtctccag gcaaaacaat
    28081 cttatatccc ttttaatatt tcaatggctc aacttgagaa aacaaaatac ccttattttc
    28141 aatttagtaa aacgttcagt aacttgtttg aaaaattaaa agcggattta ctcaaagagc
    28201 aacttatttt tttatagcgc catttgcatt tgcatttgct ttcaacacat cctgcccgat
    28261 gtcatgttaa agctccttca agacagatta aaagttggtg aatcaaaacg aatcctctat
    28321 acttttaaaa actcttatta tcaagaagat aagcattatg tatgatgatc tatggaagcc
    28381 ttatgcgtct attcaactac cctctttaaa attgggcggc attgttttaa atggtcagtt
    28441 actctctgaa acgcctattc acttagtgtt aaaggctttc aatcggcatg gtttaattgc
    28501 gggagcaacc ggaacaggta aaacgaaaac catgcaggta ttggcagagc aattatctct
    28561 aaatggagta cccagtttag tgatggacat taagggggat atttctggtt tggctatgcc
    28621 aggtcaaact tctactaaac tagtggagag aggccagcag ttacatatcg attttactcc
    28681 ccgaggtttc ccggtagagc tattgacgct tactccacaa gacctcgggg ttccattacg
    28741 tgcgagaatt gatgattttg gtcccctgct tttttcgctg atgctggatc tgaacgacac
    28801 tcaagcagga gttgtcactg tattatttga gtatgccaag gcgaatcaac tgaatttggt
    28861 taatttggat gattttaaat ctttgctcca atttgctcaa acggatagag gcaaccagct
    28921 gattgccgcc caatatggcg gagtcgcttc cacatccatt gggactattg tccgtaaaat
    28981 catcgagctc gagtctcagg gtgggaaaca gttttttggt gaaccttcct ttgtcattca
    29041 ggatttgctg agaacggatg caaatggtca tggtgttatc tcgatattaa gattattaaa
    29101 catgcaagac aagccgaagt tattttccag ttttatgctg aaattgctgt ctgatgttta
    29161 tcgccaaatg ccggaattag gtgatccgga caaaccacgg ttggttctct ttatcgatga
    29221 ggcgcattta atttttaatt attccagcaa agcattactt aatttaatag aaacaatagt
    29281 taagctaatc cgatctaaag gaattgggtt gatattttgc actcaaacgc ctaacgatat
    29341 ccctgaggct gttttaagcc aattggggtt aaaaatccaa cactcattac gtgcttttac
    29401 tgcaaaagat cgaaaagcct taaagttggt atcgcagaat tttccagtat ccagttatta
    29461 taatactgag caactactca ctactttaag tataggggaa gctttagtga gtgccttgga
    29521 tgccaagggt caaccgacac ctttagtcca agcgatgatt agagctcccg aatcgcgtat
    29581 ggggacttta agcaatgagg aattaaaagt tttagtgaat caatctcctc tttttgctaa
    29641 atattatcaa atgatagatg tgccctctgc gaaagagaaa ttgtctgaac taatctctga
    29701 gcaacaagtt gcaaaacaac agaccagtca gaaagaaaac gattcctccg tgttgaataa
    29761 tttaagtaaa aacaccttat tccgacaagt ggtacgtcaa gtttttaggg aactcaccag
    29821 agcgattttg tctttattta agattaaaag gaactagatg tgggttacct tccattaaat
    29881 tgtttaattt ataccacgca tagtcaaagt aaatgcaatt caattgacaa ggatatacat
    29941 gataaacatc acaggaacct acactgatca atatcaattg gctatggctc aagtgtgttt
    30001 tttaaaaggg cagcatgagc gcatagcgac attcgattat ttttttcgtg ctcttccttt
    30061 taaaggaggc tatgcgattt ttgcgggttt ggaggattta ctggatgttc tcgaacaact
    30121 tcgctttgaa caacaagata tagagttttt acaacatcag aatatgcatc ctgactttat
    30181 tcgctatttg cagcagttta agttcaatgg tacggtgtat acgtcgctcg agggagatgt
    30241 tgtatttcct acgcggccag tggttattat cgaagccaat ttgattgaag cacaaatcat
    30301 agaaacgtta ttattaaata ttattaattt tcagacactt attgcgacaa aagccagacg
    30361 catcaggcag gttgcgggag aacggatttt aattgatttt ggactaagga gagcacaggg
    30421 tgctggaggg tattttgcaa gccgtgcagc catagtcggt ggctttgatt cgacaagcaa
    30481 tgtcagagca gggcgtgatt attcaattcc tgtatcgggt actatggctc attcctttat
    30541 ccaaagttac gatagcgaga tacaagcttt ttctgattat gctgaagctt ggccggagaa
    30601 ttgcatcttg ttagtagata cttataatac gttgcaaagt ggtattccta atgcaatcaa
    30661 aatagctaag gaaatggaga agagaggaca tagactcaaa ggcattcgcc tggatagtgg
    30721 cgatttggca tggttagcca agagggctcg ccacatgtta gacgaggccg gtttaaacta
    30781 tgtggctatt accgcttcca atcaactcga tgaatatgtc atcaagaatt tgttagagca
    30841 ggatgcgcct attgatgctt ttggtgtggg gaccaatctc gcgattggtg ccccggatgc
    30901 ggctcttgat ggggtttata aattagcgtt tataaatggt caacctagga ttaaattatc
    30961 ggatacccct gcaaagatca ctattcctta tcaaaagcaa gtctacaggg tgttcgaaag
    31021 agatgggaca tatcgaggtg cggatgttgt ctgtctcaaa acggaaaaaa aaattgatat
    31081 gatgcatcat ccttttgaaa ttctaaaatc gctttcgata aagcactata aaaaagaagc
    31141 attactgcat aaagtgatgg aaaacggcaa acggttacta cctaaaaaat cacttggaga
    31201 aatagccagg tttagccagc aatgttttga tcgattgcct ttggaataca agcgttttat
    31261 taatcctcat atctataaag tgggtttaag taccgagctt aaaatgaaga gagatcaact
    31321 gatagctgaa cactcaagat aattttatga agactttaat catcctcgat gttcaaaatg
    31381 atttcatgcc aggcggctca ctgggggtac cgaatggtga tgcgattgtt cccgttatta
    31441 acagcatttt gcattgtttt gatctgattg ttgcttcaca ggattggcat ccctctaatc
    31501 atatcagttt cgcctccaat catactggca ggaaaccttt tgaaaaaatc aagctgggga
    31561 atttagaaca aactttatgg ccagatcatt gtgttcaggg tagtgttgga gcgcaatttc
    31621 atccgaaatt aaatactaat tctattgaag ctatttttcg aaaaggaact gaccccaata
    31681 tagatagcta cagtggtttt tatgataatc tgcataaaaa aactacaggg cttgccggtt
    31741 atctgcgcga aaaaggcgct agagaacttt atttttgtgg tttgtgcgct gatatttgtg
    31801 tttattttac aattaaggat gctctcagtc agggatttga ttgctatttg gttgaggatg
    31861 caactcaagc gcttgtgaaa gatgatttca taaaaataaa aaaagaattg ataaaacaag
    31921 gctctcacat aattaacagt tcaaatttgc ttaataattt acaataagaa ggtctgattt
    31981 tttaaatttg cagctttgag ctcaccaatt gatgatggaa ctcgctgtcg aaggaacccc
    32041 gtcgtatttt taagacccgc taccataagg tttcgtaatg atttcaagaa ggtggccatt
    32101 gggatcatta aaatataatc ctcgaccccc atcattataa ttcatttgac ctaatcgggt
    32161 ttgtcccgga tcagcccaat aatccatttt acgttcgcaa attcgtttaa aaatatcatc
    32221 aaattcagtt tctgttacta aaaaggcata atgttggggg gtaatttctt tatctgtttt
    32281 acagaaatca aggctcacat ggttgctcat ttcaacgacc ataaatgggc catatggaat
    32341 tgcttcaggc aaccccaaaa tttctgtaag gaagtgagct gatttgtccc gatcttttac
    32401 ccatacgata gtatgattca attgtatact catcatgatt ccttgttcct gtcgatagaa
    32461 gaaattattt cattactttt tatgaaagaa aagaatagcc acatcatgaa aatgcataga
    32521 atgatgtaga tggctggtaa taaatattgt gacattggaa gtaagcttaa tcccaaaata
    32581 accactgtaa ccaatcccat attgatgaag ttcataaccg cggagccatg ggctttatcg
    32641 gaggtatggc tcatggcaaa tgtcgaagca tttgcaaaaa taaaagatag tcccaaatag
    32701 atgaatatca tgggaaaaaa tagcttgata agcgctgaat gatgggttaa cgcggccatc
    32761 atcattgcca gtgcggcaaa ccctgagatt aaaatgccta attggattcc cgcgcgttgc
    32821 gagtattttt ttgcaaattg agcagaccag attgagccga gggtcagacc caaggaaggt
    32881 aaaatattgg cagcgccata ctctgctgta ctcattccta gcaaattgat ggcaataaat
    32941 ggtgccgttg ctgcgaaaat gtagacaaag caggatgcgc cacccattaa tgctccacca
    33001 gagattaatg gcttgttttt aaattgactt acatatccct ggagcagatg tttcatttga
    33061 aacgcttcga gttgaagatt tttttgtgtt tcgggaagtt gcctgaccat ggcaagcaga
    33121 atagcaccat aaacagttcc tgcataaaag caactttccc agccgtaatg ggagtttaaa
    33181 atgccgccaa gtgcaacact caaaccgggt gctatcgcaa aagccagcat cagataggag
    33241 gttttttgcg cggctaattg aggctcatag cattcattaa ccaaagtaaa agtcatcttc
    33301 agtcctacac cggatcctaa ggctaataga aatcgtccta aaacaagcaa cgggtacatg
    33361 gaaatcattc cggccaatac acatagaaga ctgctgataa tttgcaagcc aatacctata
    33421 taaagtgttt ttttccttcc atagcgattc gctgtagggc cataaacaag ttgccctatg
    33481 gcataaccaa ccaaaaaaca ggtaattgtc tgctgtgcgg catgttctgt gatcctgaaa
    33541 aatgcagtaa tgctgggcaa tgccggtgta aataggacag cattcacgga agcaaaagaa
    33601 attaacaata ataaagtcat gaaagaaagt ttggtttttg gatacatgat gagaatagtt
    33661 ttaacgatta acagttaaga tcagtataat tattgacaaa ataattaaca atatcataat
    33721 taattaactt ttattaacta tattgcaata ataaaatcga aatcatgggt aaatttgatc
    33781 aaatcactta ctttattgct gttgttgaag agcatggttt tgcagcagcc gcaagaaaat
    33841 ttggcgtatc tacagccgct gttagcaggc agattgcacg tcttgaagcc gagctaaaag
    33901 cagagctgtt ggtgagaaca actagaaaat tatcattaac tgacatcggg gctcgctatt
    33961 atcagcaatg caaaaagact cttttggagt tgaatgaggc agaaatagcg attgctaata
    34021 gtcggaatga agcggtgggc atattaaatg ttactagcag ccgttatttt gctatgagat
    34081 ttcttctccc ttatttacca gaattcatgg agttaaatcc aaaattgcag attaaaattg
    34141 aattggcgga aagatttcct gatttagctc aggaaaacgt tgatgtgctt tttggtgttt
    34201 ctatggaggg tccccctgaa ctggtgcgca agcgagtttc tacaacacgg tatgtgcttt
    34261 gcgcatcgcc ggattatctg gcaaaaaatg gtattcccaa aacaccttcc gaacttggga
    34321 agcaccgtta tatcactcac agcatgcgaa atcctgataa tttggttaca tttaaagaca
    34381 atgagcagat ttatgttgaa cctgtgttat ggctcaatga tagtcgtgct ttgtgtgagt
    34441 gcgccttact tgggatggga attatcaaat tacatgaata catagtgact gaagcattgc
    34501 aagatgggcg cctcattgag gttttgcctg aattccaaga gtccagttta tcggtgtacc
    34561 tttattatca ggcaagccga tatttacagc ctaagatacg aagatttatt gattttttta
    34621 caaaataaca ataaaagcaa gtattttgtt tcattctagc aagtctgagt tcaaagcaaa
    34681 aaagttcggg ggcacgtgct ttttttagag agagatgtca ggttatgcaa ggcctcattg
    34741 tgtcgcaaat taggggtatc tgttaataaa tgcttcaggt tgctttgtta gaatagcagc
    34801 agtcagaaac attattaact agagaatctt gaaatgtttt ttaaacttga aaatataatc
    34861 ccatactttg cacagcaatt ttagaaaaat atggtaataa ccctaaagtt ctaattttct
    34921 gattgtataa gtgtaataaa acatgataaa atgtgcacaa tttttactat ttgtttgcat
    34981 atatattaac cctataggat tgcaatagat agtgccactt tgatttaatt aatttttaga
    35041 agttaatatg cataaaaaat acaactacta cagtcttgaa aaagaaaaaa aaacattctg
    35101 gcaacatata ctggatattc tgaaggcccc atttaggcta cctggttggg tcgtttcttt
    35161 tttcttggca cgaaatatta ctcatgtggc attaaatcct aataatattc cgcaacaacg
    35221 tttaatacat cttactaaaa caagcaatcg tccagaggat gatattgttg tcattaattt
    35281 caaaaaacgg cctcctcaca aatggtttaa tgacacgctg attaaaatag ccaataccat
    35341 tgcggctctt ccttttgtaa caccaagatt acgtacaaga ttacattacg ataatgagaa
    35401 tgatatcaat catgtgaaca agttgcttgc cgagatcgat gcgcttgttc agggcaaatc
    35461 aaaacaaaag tattgcaaag gcagggcatt cgattggtca aaaattcatc taaagggatt
    35521 ggagttcctg gaccctaaaa tgagagggta tgtttatgaa caactgcatg aaaaatatgg
    35581 ttatgtctca tatacaacaa aacgcaaacc taatattgaa tttttcacct taaaaactcc
    35641 tgatggatct gaattggatt cagtgcaagt tacaggtgaa gacgaagaaa aaaaaccaat
    35701 gggggagcgc aagtttatta ttacctgtat tgcgcgggat caaaatttca tcaattggat
    35761 aaaggattta aactatacgg caaaaaatct gggcgctacg gccatcagct tcaactacag
    35821 aggcgtggat tacagtcgcg gtcttgtctg gacagaaaat aatcttgtcg acgatattct
    35881 ggctcaggtg caaaggctaa ttagccttgg tgccgaccct aagaacatct gccttgatgg
    35941 tatgtgtata ggtggggcgg tagcaacaat tgctgcagca aaacttcatg agaagggcat
    36001 gaaagtcaag ttgaataatg aacgctcttt tacttctctt tcttctctag tatttggttt
    36061 tattgtacct gaattgcaaa cagcaaattg gtggagccct ttgacttatg gtcgcttcct
    36121 actggccggt gtagtttatg ctttactcac gcctttaatt tggctggcag gatggccagt
    36181 tgatgtgacc aaagcctgga atagaatacc tgctcaggat aaaatgtatt ctgttgtgcg
    36241 ggataaagac aatggcttgt acgatggagt aattcacgat cacttctgtt ccattgcgtc
    36301 gttggttgat tctcaaatta attctatttt atataaatta tccactgatc agccgcttac
    36361 tgaggaagaa aaacaaattt tgtgtgatga tcagttttct caccatttta aaccaagtca
    36421 aagtgtttta aaaaatccaa aatacaaagg cccgcatttt atttcccgac aagatttggt
    36481 agctgagctt ggtcatcgag aggaatatac caatcatgat tatttccttg accgcttgcg
    36541 ggaaaaattt caattggatc gcgcaacacg gccagtggct ttggctgaag atggggaaaa
    36601 ggatatagat gggatttcat cgcaactatc taataataaa gagcgtccct taattattgc
    36661 cagcagtgga ggaacagggc atatctctgc cacgcatggc atcattaatg atttacagag
    36721 taaaacagat aatgttgtca ttacgcagca tcatgcggaa ctctacaaaa acaagccctt
    36781 ctcaattaca tctgttctga ttcgtattgg tgtttggttt acctctcttc ctatcctgga
    36841 agacatactg aaaggagtta tgaggtttat cggatatccc gttttaccga gctcctccat
    36901 cttttgggat caaatgtcta aaatacagca gagtgaaact aaaaaagaaa atggaattga
    36961 aaccggcaga acccgtcctt atgtggacat gctgttggat atatacccgg agggatatga
    37021 atacacggca ttcaataacg cgactcactt gacttccagt attgaagaca ttcaaacgat
    37081 gataagcttc aaggggcatg ttgaagagga taacaggaat atagtctatc aaaatatatt
    37141 acaacgcctt atgcatgcgg cgaaacagaa tactccttac acaagattaa tttccacaca
    37201 ggcgctttct ttgggagcta tttgcgatgc cgttaaatac tacaatacgg tatttttgcc
    37261 tgtatacaat gctgaaagag ggacttccta tcagcctatt gccattgatc agtatatgac
    37321 cgatttgcct tcactgggtt gtattcattt tatgaataat ttggaggagc tcacatcaga
    37381 gcaaaggcaa ttgatggaga tacatgcagt aaatatgagt gaaccattta aagaggcgca
    37441 ttttggcaaa gaacaaggct ttaaagcggt tcataatatt gacccacgca ataaccctat
    37501 gattcgcaat gcctttaaag acccttcatt gacaaaatac ctggataaga cccagtcatt
    37561 tgatttgcac tttaatgttt ataaaaagga aaagcaaaat gcactgccag tactgaatgg
    37621 taaagaaaaa attacaatca aaccgcatgc taaaattgct tccatcatga taggctctct
    37681 ggcggcaaat gcttcagctg attatgcgaa gtatttattg aatcaggggt atgagcatat
    37741 ttttcttttc ggcgggctta atgattccat tgccgcacgt atcgatcaaa tcatcaattc
    37801 ttaccctgcc cccacgcgtg atgagatacg taagaaaatt attctcctgg ggaatcaatc
    37861 tgatgttgaa atggcaccaa taatgaccag aagtaactgt gtggttatcc gtggaggtgg
    37921 tttaagtgtc atggaacaaa tggccatgcc cattatggat gataaaattg tattattaca
    37981 tcatgaagat aatgaagagg gccctttaac atctggtctc agttgggaag atgggaattc
    38041 cgataaatta atcgagtatc tgtctgaaaa aggggcttac gctaaaaaaa catcgcctgg
    38101 tctgtgttct ggtcatcttc atgaggcaga aaagagtttc gaaaagaaat atcatgggca
    38161 actgaagtcg acagaaacca agaaaaaagt agatttaacc attcctcaac aagagacgta
    38221 ttctctgaaa aaagagtggg atagaaaaac tggatatact gaatctggac atatactatc
    38281 ccatcagcat agatttttta acacgatacc tgaagtaaga gagccattct gttctaagga
    38341 agatttacat cataatgagc tttccagtca aagtttggtt agtgtcagtg ctggttgagg
    38401 aaatatgcct ttaatcatcg aagctcgttt gattcaggtc agtactagtt tgtgattgca
    38461 ataagtactg attgatgcaa ttccaaaaag cgagattgtt tgttaataac acatgtcgaa
    38521 ttttttatgt tattttagta taacccaatt actttttgat aaataaaagt cgtttgttgt
    38581 cattaattgc atgttttaat aatagagaaa tcaatatgcc taaaatttta gatagccatg
    38641 catatcgcga gatggaaaat gagctcaata ctcttttcga gcaaatttat aagcatggta
    38701 aaaatgacag aaccgcgatt acattacaag ctcagcaatt tttagataat ttgagtgaat
    38761 ccataaggga tcattcccgc ttattgctta ccaaaattgc cagagaagaa ctcatttatt
    38821 ttaaggaaaa gaaacctggg gattcgcgag agaaatttca aaatatcgat aagatgagtg
    38881 attactttaa caatttatct gcattgatta atttctcggt gatgcagcaa gaagatccta
    38941 ctctgcgggc tttttattat gatatgtata ttcaactaat gaatttttgc tatcttaaag
    39001 gggatttgtt tggagcgggt gcaatttata caggtttaat agccaataac ctccctaact
    39061 ctattgattg gaagcaattg agttccgaga gtcgcttgat ttttaaagat tgtgaagaaa
    39121 aatttaagcg attatttaat ctctcaacga catacaatgc gcacactgat ttaaaaagac
    39181 aattcaaaac tgctttgata cccgcattac cgtcacttct cacggttcag atatatacta
    39241 aggactctta cgataactcg gtcagcgatt ttgaaagaat tcaggataag ttacgtcagt
    39301 tggatataga acataatcgg tttcttaatc aattacaaaa tgaccgttca acctggtcta
    39361 aagcttatta tgattatatg agtaatgttt atctcccgca aagcatattc gaatacacta
    39421 ctctcttaag agagtatgaa gaacttgtta caacgcatgg tggccctatt gcgaagcagt
    39481 ttcataagga taagaattca gaacctctgg taaattttgt tgaatcgacg ctatcagaaa
    39541 atcaatcagt tctcaaagta tctccttttc aaaatgaaag aattgttgaa atgctttccg
    39601 agattgacgt ggtaaaaagt gagtgctcaa gcccagctgt gtttgatgac ctctcctgga
    39661 agttgagaga aatgaattgt actttggtaa aagccaaatc tattggtgat tatacttcca
    39721 aaatttttgc aaatcttgat actccataca tggaagagaa tacggatgaa gacccgaaac
    39781 cagtgcttgt agaagagctg caaacggatg atgtgtacca acttcaccat agtggtatga
    39841 aattgatgcg tgagctcgaa caggctatag aagatcataa accaaaggtt atttatcatt
    39901 gttcaggtga ggcaaactat ggtttcttta aggaagccag aaggaggggg agacatttga
    39961 gtcgagaacc aatggatatc gaagtcatgg atatggatgt gatggattat gatgagaatg
    40021 atttacgtga ccaaaccgtt atgattaccg tgtaatttgt cttgcatcta taattgcaat
    40081 ccatcggata gctcttatag atgccaatgc gatttttatc ttattttgtc ctatttcctt
    40141 gcagaagttt ttctatttct tctgagggta taggcttact cattaaaaag ccctgaatgt
    40201 cattacatcg ccttttcttg agaaatttga gttggttttg cgtttcgact ccttcggcga
    40261 tgatattaaa attcatgctt tgtgcgatag caataatcgc atcaatgatc gcttcatctc
    40321 ctggcgattt tgaaatattc tgaatgaatg attggtcgat tttaagacta tcgacagcga
    40381 cctgttttaa taaattcagg ctggaatttc ctgtgccaaa atcatccaaa ataatcaaca
    40441 cacccagtga tttcaacttt ttgatcgttc tttgtatttc tttatcaata atcacatttt
    40501 cagtaatttc cagctccaaa caatggggtt taactgaata ttcttttaaa agatcctcca
    40561 ccattgcagg gaaatctcgt tgtcttaact gtttacttgc tacattacaa gcgacacgaa
    40621 tttcaggcag ccccattgtg cgccaggtac caatttgttt acaggtttcc cgcaaaaccc
    40681 actcccctat cggtacgata agccctgaac tttcggcagt aggaataaaa tccaagggta
    40741 aaataatacc tttttgcggg tgattccacc ggattaatgc ttccatggaa cgaatctcat
    40801 gagtattaac tgtaaattga ggctggtaga ggaggaaaaa ctcatgatgg ataatcgctt
    40861 tttgcaagtc taattccagc tgaaattgct ggtttgtttt tttgttgagt ttctctgtat
    40921 aaaatgagaa ttggttaccg ccgcgaaatt ttgcctgata catcgccata tccgcattac
    40981 tgagtaaagt gtttactgtt ttgccatccg ttggataaac gcttataccg atgctgacat
    41041 gaatattgat gtctctttcc gcgatatgaa atggtttttg aaaggatttg aggatttttt
    41101 gagctactcc ggcaatttgc tcttcagaat taaacgattg aaaaagcatt acaaactcat
    41161 ccccaccaag tctggcgaga gtatcttctt ttcgcaccaa agatcgtaat cgtttagcaa
    41221 cggcacacag taattgatcg cctgcttcat ggctcaagct gtcattgatc attttaaagc
    41281 ggtccaagtc aaaaaataat atggcaaatt tcattttatg gcgggcatag ctactgatag
    41341 catgttcaat acgatcaatt aataacaagc gattgggtaa attggttaat gcgtcatgag
    41401 tagcctgata ctccagtttt tcctttaatc gcgtttgttc agtgatgtct ctgaagctcc
    41461 atacacggcc tactgtttgc ggaccaacgc gatgcggttt ggaacagcat tcaactactt
    41521 taccatttct aaaatgataa atttgtatac tggaatcatc gatgtgaata gaaaattctt
    41581 taacatgttg ggcatgctgt tctgaattga gtaattgcgt tgacatatac tgaatgagtt
    41641 gggattcact ggtttttttc atgatggatt cggggatatt ccacaatttg acgaattgag
    41701 agttgtaatc aacgattttc ccttgtaaat caacaaccag aatgccatcc gaagaggact
    41761 caatcgttgc ccgtaataat gagaaggatt gttgtaaaga ttcggttcgt tcttcgacca
    41821 attgatttaa aaattcagtg tgtttttgag tttctctggc aagtacccat ttttgtgtca
    41881 aagcacttgc cagctgtctt actgcaatca catcaaaggg cttttttagc accaataaat
    41941 tatcacttaa acccaaggta tttactgtgc tttcccatga gtagtcggaa taggcactgc
    42001 atataacaat ttgtatttgc gggtcaatgg cccaaatgcg tttgatggtt tctatcccat
    42061 ctatccccgg gggcattctt atgtcaacaa aagcgagtgg gtatggccgg ccttcttcaa
    42121 gtgagcgttt cactttttct atgccttcaa tgccttgagt cgctacatcg atttcaaatt
    42181 caggcaaaga aatcccaagt tgttctgtct catcatcaaa cagctcggca tcaaatttat
    42241 ttaataatac agagttcttg ctgacattta agattttcat aaaatcctga tggattgccg
    42301 ggttgtcatc aatgaccaga atgcgaaaag attcagtact catttctacc tcgtactcaa
    42361 ggttttggga ttgatgggca aatctaatac aaattgagct cctttgccat tgccattact
    42421 ttcagcataa agtgtaccac ccagatcccg tgcataaatt gcagaacagt gcagtccaaa
    42481 gccgtgacca tttttcttgg ttgtaaatcc aaaagaaaaa attcgatcca ggttatcttt
    42541 ttgaattcca attccattat catttat
//
LOCUS       DACTJE010000006        40211 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.006, whole genome shotgun sequence.
ACCESSION   DACTJE010000006 DACTJE010000000
VERSION     DACTJE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 40211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40211
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.006"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            50..2086
                     /locus_tag="I9760_000235"
     CDS             50..2086
                     /locus_tag="I9760_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HAT5042033.1"
                     /translation="MKFKIATCIVTMLSSFIVFSSNSNFSDNNTNDLLHLDWRDTSVS
                     PAVDFYTYANGNWKKNNPIPADYASWGSFYVVNEKVQNIIHQMLINASKNTHAKPGSI
                     EQKVGDFYFSGMDVDSIDKSGITPLKPEFTKIEEIKNLKDLQAEIAHLHKIGVGPLFN
                     FGSMQDFKDSTSMIGAVMQGGLGLPDRDYYLKKEAKFKQVREAYVNHLTKMFGLLGDS
                     PEMAAKEAKIVMDIETQLAKASMSQVEQRDPHAIYHMMTITELDKLTPNFSWSQYLSA
                     RGQGKIKKINFAMPDFFKAMNELLQSVSLDEWKIYLRWHLIDAFAPYLSKPFVDQNFK
                     MVSALTGTQKLLPRWKRVVNTENGALGFAIGELYVEKYFSPESKQKVLDILKNIRAVL
                     QEDIKTLSWMSPKTRDAALKKLDLMEERVGYPTKWWDYSSLKIDRGPYVLNVIRANEF
                     LINRDLDKIGKPVDRSEWAMTPQTINAYYDPSMNNLNIPAGILQSPFFDPKAPTAINY
                     GAIGYVIGHEMTHGFDDQGAQFDGYGNLKNWWAPNDLSKFKNATQCIADQFSKYVVDG
                     DLHVQGQLVVGEATADLGGMILAYKAFQRSKEYKNAKTIEGITPDQQFFLGTAHVWAM
                     NVRPEQLRNQVTTDPHPPAVYRVNGSLANMPQFQEAFNIPNGSPMISKNRCIIW"
     gene            2240..3091
                     /locus_tag="I9760_000236"
     CDS             2240..3091
                     /locus_tag="I9760_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAT5042034.1"
                     /translation="MCRFVAYLGRPTLLENVLVRPKNSIIMQSLHARETKYRTNGDGF
                     GLGWYSPEVSSDPALFTSVYPAWNDRNLLHLTAKIQSPCFFAHVRSASAGGVTNYNCH
                     PFIYGDWMLMHNGEINDFIDVKRHIRHLLDDDIYHWVKGQTDSEHLFGLFLQLAKGRD
                     ITQLSVAADVLQETINKILEVIGQFGHSGPSYFNICLTNGKKIIATRYCSHEKKKPLT
                     LHYLIGYVLSTQGLWIKEEGNPAYVVVSSERLNDLDEGWEDVPPQHMILIDENKNIQL
                     RPLQTLG"
     gene            3206..3622
                     /locus_tag="I9760_000237"
     CDS             3206..3622
                     /locus_tag="I9760_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042035.1"
                     /translation="MSFSLSELLHYKNEKIVTHFCYSHPEYSIQEGLFLFEDLLAWMW
                     LNKQRRLRGKKTYLFGPLLILDEMWHSFILHTRDYIDFSMKYFGEYFHHDVEPVGFEH
                     VLDEEELADYLQDCFQYLNKEWVERRFSIAFAEMDD"
     gene            complement(3678..4475)
                     /locus_tag="I9760_000238"
     CDS             complement(3678..4475)
                     /locus_tag="I9760_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5042036.1"
                     /translation="MLKQIVLTGLVIIGIVITLMYLFQRHLIYFPNRHTPKLEDYNAS
                     DMKVVSLRTKDNLHLKSWYKPASKHRPTILYLHGNAGHIGYRMPLVREFIDAGLGVFL
                     LEYRGYGGNPGKPGEKGLYEDGETAIEFLIQHGVPSKRVILYGESIGTGVATHLATKY
                     PVCAVILQSPFTSLTRLAQYHYPLNFLKPWDQYNSLARMKKINAPILVLHGKLDQIVP
                     YQEGLNVFNEANEPKKMVSFDDKEHNDLWSADNFSREIIQFALNQCS"
     gene            complement(4776..6080)
                     /gene="lem28"
                     /locus_tag="I9760_000239"
     CDS             complement(4776..6080)
                     /gene="lem28"
                     /locus_tag="I9760_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem28"
                     /protein_id="HAT5042037.1"
                     /translation="MYSDFFFTIMLKYSTNMLELAMPKINKIVNGTDLTPHYLSEPNK
                     EFKIYRYNNEVYAVRFENDEPMDYVLMWKSHKSEHTQNSEMIKNTEEDYKELGKGEQG
                     TVYEKTEDKAMKVSRGRHPREFYEEINLHIIEQQFFLKYHGIQEHFVLGLWNIKNEEN
                     VYFYMPKINAIPINKKIDQPKIEEFVLALKELNDAGYWHPDLANNPYHISPQNLIATE
                     EMVKTIDLDGGFRYDKGRVDELSRKSLVYGKDQWLYVYNFIYPPTDEEDHRIDWRVPI
                     EKWYENNRDESLSDNPHTLLRFYHEGLISLPKKLAHDLHETILEELSQDKKFKKHSHE
                     VGVNRFFSPKKQEIYEGLKGDGLKKVILKELRDSLAEIRTMEQLEEKKLEFLASPEMQ
                     ILAEGQDKTTKALNLKTSSRKKVMVIFKEAEERILNSPNVSI"
     gene            complement(6098..6520)
                     /locus_tag="I9760_000240"
     CDS             complement(6098..6520)
                     /locus_tag="I9760_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HAT5042038.1"
                     /translation="MKSISQCLNKQLSTICQHSMQLEELSLKISAFLPSHFISKCQVG
                     SFNKGCLVLTTTNAAFASEIRYLLPELRDKLRKEAGLYQLSSIKITIIAPSSQIENPA
                     QKKSLTLSEKAKATIISESQHCTYEPLKKALLHLAQSE"
     gene            6651..7091
                     /locus_tag="I9760_000241"
     CDS             6651..7091
                     /locus_tag="I9760_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HAT5042039.1"
                     /translation="MAENTTESNVIYFTRLHWIIFFGPFVGLGLVMAIYIYIIQLREV
                     SLFLGFFALIWILITWVTYHFSSITIKRKQVILRTGIIVRQTVDIPLNKIETIDIRQS
                     ILGSILSYGTLIITGTGGTRYLINYLYKPLTCRRYIEQLMNEPH"
     gene            7227..9200
                     /locus_tag="I9760_000242"
     CDS             7227..9200
                     /locus_tag="I9760_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAT5042040.1"
                     /translation="MNYRSDIDGLRAIAILFVLFFHGGLTLFPSGFIGVDVFFVISGF
                     LITSIIEKSLQNNRFSFMDFYSRRLWRLQPVFICLIVVTTLVTLFYYLPDDLMLFSKS
                     ARKTSIFTSNVFFKNVTSDYFSPDSNQLPLLHMWSLSVEWQCYFILPVGIYLLYRLVG
                     PQHRIKITYLLTITFFALSMYYSWSNPAKTYYQFSSRIFEFLIGSCVASASGSKRFLA
                     NKYVLNFITVLAVLTLFYIAMHHDINIGFPNWYTLILCLATAILIAAGQQEPQPIVTY
                     ALSLRPLVFIGLISYSLYIWHWPVLALIRYLNIEESNLVLLVTFGLISIIAYLSWRFI
                     EKPARKLHRIRFSYTVVCLLIMPVFVTHLSDYLIKKNAGYPYRFNEAIQVYAELNKYK
                     NPQRPLCLERKNTEVNPQCVLGAKNSDSRTGFMIGDSYSNQHWGFMDTLAKEANVSIL
                     AHATVACLALPDIYQFDWNVKNKIYQECHDQTNRYFNMIKTNHYDYVILGQNWNGYFG
                     DRIINQLNDNRSQELSLKRIEKALDKALELITTSGAKPVLIKSIALSKGNPYNCFFEH
                     IKLHKKYNPERCDFNLDVSEQIWFDNLFAKMQRKYKTLVIIDPRVAQCPSGMCKVDIN
                     GIPVFRDSGHITDYASYHLAKLYLEKNNNPLVS"
     gene            complement(9302..11581)
                     /gene="topA"
                     /locus_tag="I9760_000243"
     CDS             complement(9302..11581)
                     /gene="topA"
                     /locus_tag="I9760_000243"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAT5042041.1"
                     /translation="MSKHLVIVESPAKAKTIQKYLGNDYDVLASYGHVRDLPARKGSV
                     NPEKHFSMTYVPIEKNARHIDTIAKTLKKSDSLLLATDPDREGEAISWHIFELMKERN
                     LLKDKSVHRIFFNEITKAAIQDAINHPRSISMDLVNAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLIVEREEEIERFIAQEYWKIIAKCAHASTEFEARLTHYNEE
                     KLQQFSVTQQEQAHEIKKQLIAQAQGFLTVAQIDKKQRKRKPSPPFITSTLQQEAARK
                     LGFTARKTMMVAQQLYEGIDIGTGTVGLITYMRTDSVNLAKEAIDEIRDYITQRYKGD
                     NCPNSPRIYKTKSKNAQEAHEAIRPTSIKRTPEMVQGSLTSDQLKLYSLIWKRTVASQ
                     MADAILDTVSVDFSCGKGNTFRANGSTIAFPGFLSVYEEGRDDSKDEDNEDKILPAFN
                     VGEKIKVSDIETNQHFTEPPPRYSEATLVKALEEYDIGRPSTYASIIHTLQQREYVVV
                     EKKRFLPTDVGRIVNRFLTNYFTRYVDYQFTAGLEDTLDAIARGEKDWIPVLEEFWQP
                     FVQQIQNIDEQVQRKDVTTELLDEKCPKCQKPLSIRLGKRGRFIGCTGYPDCDYTQDI
                     SNPEGEKSEPEVVEGRSCPLCHGALHIKTGRYGKFIGCSNYPECKHMEPLEKPSDTGV
                     TCPKCSQAKILQRKSRKGKIFYSCGNYPKCDYALWNEPLDLPCPKCAWPILTLKESKK
                     FGRQILCPREGCDYSAKED"
     gene            complement(11660..12097)
                     /locus_tag="I9760_000244"
     CDS             complement(11660..12097)
                     /locus_tag="I9760_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HAT5042042.1"
                     /translation="MKDNLFEMLLNLFEKSLTQLQKSHKSNDQDSQSQLLEEDLLSAE
                     EQSLFIKSAQEKSTRIFTYDEQMKLTKASYQFLMRMKLWNILDIDQFESIVNQLQFSE
                     SRIVTLQETKWTIRNALASSLNEEQLTFLDLVLYQTEDELTIH"
     gene            complement(12087..13172)
                     /gene="dprA"
                     /locus_tag="I9760_000245"
     CDS             complement(12087..13172)
                     /gene="dprA"
                     /locus_tag="I9760_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAT5042043.1"
                     /translation="MNNLQPLLALNRMKKIGPRTVLKLQKRWPDLNLMFQLSNVDLEE
                     AGLPSWLAQTIKNFDPGFIKTDLNWLSSSENHHILTWDSPYYPALLKEIADPPFILYA
                     KGELSAMTQPNLAIVGSRNASVTGNENARAFSREISRHGVSIVSGLALGIDAHAHLGC
                     LEGGGKTIAVLGTGIDCIYPRRHLKLAEQITENGLLLSEFPLKSPPIAGHFPLRNRII
                     SGLSLCILVIEAAIKSGSLITARMALEQNRDVLAIPGSIHNPLARGCHYLLQQGAKLV
                     TSVADVLDELKIEHHQFTSNKPIFSLASGKENLVKFIGFETTTIDQIIDRSGYGMEQV
                     TSGLAELELKGAVMAVPGGYIRCEYER"
     gene            complement(13169..14206)
                     /locus_tag="I9760_000246"
     CDS             complement(13169..14206)
                     /locus_tag="I9760_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAT5042044.1"
                     /translation="MRYCLLIFCFIISSITHALSLRPDSPSRYVVQPGDTLWSISSRF
                     LNNPWEWKALWRANPQIKNPDRLYPGAVLALEYYQNIPYLRVLSNGTIKLSPNIRVTP
                     QEDAVPPIPLGDIKPFLDESLILDVNVLNRAPYVVALMSERMLGGQGDEIYVRGLHPS
                     KEMPQGGTIGYSIFRGGRNYFDPITHELLGYKAVLVGYGELVAGGEPATVLLTSINQG
                     IKINDKVLINNHPEFELYFEPETPARQVRGYIIEMPDNMPVGNTQEAVGGVIIINLGE
                     TSGIKPGDVLAIYGKERIVNDPQNHLRPITLPQERLGEAMVFRVFTKASYALIVRSTR
                     AIHLLDTVTNP"
     gene            14300..14812
                     /gene="def"
                     /locus_tag="I9760_000247"
     CDS             14300..14812
                     /gene="def"
                     /locus_tag="I9760_000247"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAT5042045.1"
                     /translation="MAIRKILYLPDERLRKIAKPVETFDESLQTLINDMFDTMYDARG
                     VGLAAPQIGVSLRLSVIDIVGDKKEQIVIVNPEIVSSHGEKEFEEGCLSVPGAYDTVV
                     RAEKVTVKALDRFGKPFEITGEGLLAECLQHEIDHMNGKLFVDMLSPLKRMMARRKLD
                     KFKRLQARKP"
     gene            14809..15753
                     /gene="fmt"
                     /locus_tag="I9760_000248"
     CDS             14809..15753
                     /gene="fmt"
                     /locus_tag="I9760_000248"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAT5042046.1"
                     /translation="MNGLTVVFAGTPEFGLPCLDALIQSRHHLKAVYTQPDRPAGRGR
                     KLQESPVKEWAINHQIPVYQPLNFKNQEAVDELSALKPDVMVVIAYGLILPKAVLEIP
                     RLGCINVHASLLPRWRGASPIQHAILHGDAESGVTIMQMDVGLDTGPMLCKAACPVTS
                     SDTAGSLHDKLAKMSVKPLLDVLEALASNSAQFELQNNELATYAGKINKEEARINWHQ
                     SAVEIDRKIRAFNPWPVAYTLAGELMLRIHQAKATDIMSTEMPGMILNIDKNGMLVAT
                     SDNALLVEKIQFPGAKIISVRDWLNSGKTQLHTGLMLQ"
     gene            15750..17033
                     /gene="rsmB"
                     /locus_tag="I9760_000249"
     CDS             15750..17033
                     /gene="rsmB"
                     /locus_tag="I9760_000249"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAT5042047.1"
                     /translation="MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFC
                     RHYFRLQSIADTLINKRPKDNELWIVLLIGIYQLQYMNKPDYAVVKETVSLLGQVKKS
                     WAKGLVNAVLRNFCRRKDHILEKLAAEPVFIYGQPNWLLQRLQKDWPNDWQFIAAAND
                     RHPPMTLRVNVLKHSVDEYLDLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAQGS
                     VSVQDAAPQLAAYLLSLKPGQRVLDACCAPGGKTCHILEKEPGLASCVALDLDPQRLQ
                     RVRDNLNRLDLQATVVQGDVLEPRSWWDGHPFDRILLDAPCSATGVIRRHSDIKLLRT
                     DEEISAISQVQSSMLNSLWPLLAPGGLMVYATCSIMSDENELQIAQFVAEHSDCQVSD
                     GKWSWGRWTGHGQQILPGEHSMDGFFYSVLRKDEK"
     gene            17030..17779
                     /locus_tag="I9760_000250"
     CDS             17030..17779
                     /locus_tag="I9760_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT5042048.1"
                     /translation="MIIDWPVIIVLFCLSIPGVLIVMHRLVYFLLADNTERLKKRFSR
                     FVVLQTLFMTFVMSFAGSILSSRTGLGDPLLEGLLQGKASLDSFQVILLPTFLYALLG
                     LVIFCILYYGLFGSILDEKSFKIMSELRSKIGLDGCVLYGGVVEEVIARWGLMNVVCF
                     FALLFTSQINSEIIIISIFLSAFIFAVGQLPAYIAAGCTPSRRFLYSFVLLSLSQSIL
                     FGFLFWQYGLTSAILAHMLFHLGWDRYEKLF"
     gene            17952..18449
                     /gene="ceg33"
                     /locus_tag="I9760_000251"
     CDS             17952..18449
                     /gene="ceg33"
                     /locus_tag="I9760_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg33"
                     /protein_id="HAT5042049.1"
                     /translation="MAAQLDPSSEFAALVKQLQREPDNPGLKQAVVKRLPEMQLLAKT
                     NSLALFRLAQVYSPSSSQHKQMILQSAAQGCTNAMLSACEILLKSGAANDLITAAHYM
                     RLIQSSKDSYIIGLGKKLLEKYPDFAEELKSKEVPYQSTLRFFGVQSESNKENEEKII
                     NRPTV"
     gene            18566..19357
                     /locus_tag="I9760_000252"
     CDS             18566..19357
                     /locus_tag="I9760_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAT5042050.1"
                     /translation="MQTRFTYLPVTSLQAGQYQPRQDFNVTELQELAQSISSQGLIEP
                     LVVRPIAKERYEIIAGERRWRAAKLAGLNEVPCLIGDYSDQQACALTLIENIQRQDLN
                     LIEEATGYKRLIEEFHYHQDEIAALVGKSRSHVANILRLLTLTDKVKQLVRDKVLSFG
                     HARVLVGLNPEQQEMFAQQIIGEEWSVRHLEQEIKSYKNKDINSPKNPRKDRDIERLQ
                     TILAEQVGAPVQIVNDGEDGGWLKVKFFDNDTLAGLLERLGLRYD"
     gene            19449..21242
                     /gene="dacB"
                     /locus_tag="I9760_000253"
     CDS             19449..21242
                     /gene="dacB"
                     /locus_tag="I9760_000253"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HAT5042051.1"
                     /translation="MKRTLTGAFMWAVWSLSSHAASMQFEVDKLINRLNPHVNLGIVV
                     TDLTSGETLYKRNANRLYIPASNMKLFSEAAALMALGPDYQFKNQLSTSANQLQQGVL
                     HGNLYLHLSGDPSFSREDLKTLLSSLKDWNITTIQGNVIIDSSLMSIPAYPPGWLTSD
                     LSYSYGAPIAPLMVDSNRLTITVNPGAKAGAPAIVEVDDGGGTINLNNQATTKASEKG
                     CGVGFYLDPENNLTVRGCVGLGQWAVQQRIAIKNPFVYAQGMIVSELAKSNIKLNGQV
                     LLGRAPAGTLLIATRYSKPISQLMADTLKPSDNLYADSLYLHAAAKIKGSPVDWKQAQ
                     PVIKNFLQQQTGIDLKDSNFTDGSGLSRYNLVTPAQTMALLKFLYQRFPLSYEYIAAL
                     PISGRDGTLQKRFKTPNQQGFVRAKTGTMTGMNSLSGYLYTANGHTLAFAMYINRLPG
                     KPAGPGRPLLDALCTYFLQQSPTSSRLARVLSPHSRIKFQFNPTQIELQRVHQAKWRR
                     LETAVRQVLRGQDVNVVYRGNELIVTDNQSNANSVWKALQSIGKKYSFAVALSSKVMP
                     VTPSGKPLLLWVQAPLSENKAERTWIIREAV"
     gene            22031..23188
                     /gene="legS1"
                     /locus_tag="I9760_000254"
     CDS             22031..23188
                     /gene="legS1"
                     /locus_tag="I9760_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegS1"
                     /protein_id="HAT5042052.1"
                     /translation="MNFSIRVILLLTGFVSLAFSAPKFLTISDIHYGAENIAKDGQDT
                     GKEFLDVTFKRFEELSQNVDFILHLGDLPTHSLFSIPKKEEYEQVVFHGLHEANPGQK
                     PMFYITGNNDSLLGNYQPFSSDNKSPLNLALDWDGACAYCDGLIIDDTHMRKDGYYSS
                     YVIPNNKEIILIALNSVQWTKTPVFLPKYPNQQRDAFVQLFWLEQQLKNHRAKQLLLA
                     MHVPPGTAYNGNRFWHDIYLDRFLTLLDKYHQSYDQITILFSHSHMEEFRKIRLSDGS
                     TIYAFSTPGISRAHHNNPGMKIFDLDKQMRVRNSTTYYTSDLYEWGNEYYQAMNTPDA
                     IFPNCQNKTIPQCLDTLSPEQVCNNLEVGLFYGVKSTRVPYQGCIKTYQIN"
     gene            complement(23205..24755)
                     /locus_tag="I9760_000255"
     CDS             complement(23205..24755)
                     /locus_tag="I9760_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAT5042053.1"
                     /translation="MAQKPTKITHLVVLGDSLSDRGTLNKRELLGFIPMSYLSGLRSK
                     SPKGRFTNGFLWGDYVGATTAEQFEIEHIRRKLRLHNNARDNADIGDELLINDKARRE
                     NENSFSLNEDNHILFKGERFARFYCEGGLTSHDYSCSFTFNLVLFFTRLILATLGGKR
                     TQLLADDKKYNISNLEKSETLVVEWSGANDLITANDKPSRAAAEKAVNDRIQNIEALI
                     HNGYRNFVLFNLPNLSLTPRFQRKNAEEQRNASDCTEYFNQQLQTKSLELIKKYKELG
                     IPVNLSVFDVNEQFKEVYSNPEQYGFDRDKLKSPYVESDLFKENQKNPVDQKEHISPA
                     KGYMFWDDVHPTATMHDWLAERFKEKYYDKVFRFEPPLQAKHRHKETADALAKTTDSM
                     VKTDKPVKKLPENIVLMLNAIHSRAQLMCQSTDDQRREKGELLKHFIFEIKCQHGNLE
                     NIYGLISAFTLNPDHSKIIKTHQNPIIDFFRNKATTRSEDDIAALQKAVAAYLQPEDN
                     KEHLVKLT"
     gene            complement(24976..26070)
                     /locus_tag="I9760_000256"
     CDS             complement(24976..26070)
                     /locus_tag="I9760_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HAT5042054.1"
                     /translation="MRVSKLLFLTLALSGNLFAQDIKVVGTLNQTLKAPQNKTLNTKP
                     AIQQIKLLKMQLSNSAIKALARKTDITLKKPNTITTSTALPSKVELGMNNVPVLNQGS
                     FGTCVTFATTAAIDAALNKGDYISQLCQLQLGLYLEKNGHSPSGWDGSLGRIVLSQME
                     NFGIVSKEKQKNLGCGGLTEYPDNEQPTPDHAMSLEQFHEMSEDPETNPVIYTPILDV
                     YQAVLDRTDTNKTINDIKTALNQKDRVTFAVLLLDFDLGVMGAVGTNKTQFDTWVLTP
                     EIARDIYLRPFFGGHEMIITGYDDNAVAVDDNGREHKGLFTLRNSWGEQFGDKGNFYM
                     SYDYFKVLTIEAQRIRALADNESEEESMTA"
     gene            complement(26272..27003)
                     /locus_tag="I9760_000257"
     CDS             complement(26272..27003)
                     /locus_tag="I9760_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAT5042055.1"
                     /translation="MKEISALFFLYLISTASFSLPKGFVYLHDVAPDIIEDIRYATHN
                     NFIGNPIPGYKRGVCIVTRQTAQQLKKAEEYIKPKGYTLKVYDCYRPQRAVDYFYKWS
                     RNAKDHRMKPEFYPREIKQDLFDRGYIALTSGHTRGSTLDLTLVKLTASREKKSNHPL
                     TRCYDKTPNYLNDDSIDTGTRFDCLDVSANIDYSKLSKNQKANRELLQKLMKRFGFRP
                     YYYEWWHFTLKNEPYPDTYFNFPVE"
     gene            complement(27029..29767)
                     /gene="sidF"
                     /locus_tag="I9760_000258"
     CDS             complement(27029..29767)
                     /gene="sidF"
                     /locus_tag="I9760_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidF"
                     /protein_id="HAT5042056.1"
                     /translation="MPRITENIETYVTHLLSDLEPVPSQNQHLAYGYPGERQVFKDPM
                     LDGKQVVVVNSQYDKHGRPVTGQPDVIQEANNYIDNLVAAAKSLIDKDKKGEKDLRKN
                     AIDEMAKTFKKSISETIPSDKAKADLFSSKKSSANKDFLEQLAKVEGSLQQFTQAVAK
                     ASGHKLKALDKEGHNIRSHNRDTLIHRFKAPNSKEGEEQFIMYIPCGRYTKRQQRLMG
                     KDESEVNRDHDTTSHKRSDLVLGNSSMARMIAGTRHSDGTVTIHHDSFSGPGARMPYS
                     DFKGADDYKKLAIKAVTLINQEEVIQTLAQRQIDRMTNEDLWNKIPEEYRPDELPPDA
                     EKARAQLIKLYVEHNPLSVTECYTQVVTAGQRVAAENQKEQFEYVRQMMDAFDGSKAK
                     ITIQTGSNTEVETAVGYQARMSSWGVNWFRQVGALNPLSDNSVTKNQNARFVNQMTDD
                     VIRNLDKVAQNLGDYDKAGALHTLLKGPDVSDLNQQITEKENALKEVKGAYREALFSY
                     FEEYQKGEGKWDQAKLDQLKNQVDGYEKSIKKQESAIYELHNQIDALRKAYYTEHKGQ
                     INKALQELKEQISPVIQNKETDPETKSRLQHFYNSCAYLTQAQELYYENTWHHGKNNF
                     KLQTLMASLSCELDYANTKGCKSNNDRGQRLAQKIVGNALWTAMSEDGLYTGEFLDHR
                     RTHGKEVSNVEQLDRELTTIQALHHTANTGVSGGKFEIQDKANFADNGLFGKVANFAK
                     IKEMGPENAFTKNMGTKIKAGLGLGLLAAGGFVALTLLFPPAGLAVAALGIGVAAGAA
                     IVAGVTFAATYLFGTAIDAINNKIEEGKIKSIVREGMKAASENSPQHKTEQKDHLTVN
                     AKLHQQLGVTRESVLERQSTPKQEFEKPKDMTSKVDLTAAMEDDNRSESTPTPVNF"
     gene            complement(29949..30128)
                     /locus_tag="I9760_000259"
     CDS             complement(29949..30128)
                     /locus_tag="I9760_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042057.1"
                     /translation="MKWIMLIVVIITTAGCCCTTAEVVEYQQVTVAAPVVEPVILDVD
                     DPEPLDVTTTTIEFY"
     gene            complement(30251..30517)
                     /locus_tag="I9760_000260"
     CDS             complement(30251..30517)
                     /locus_tag="I9760_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HAT5042058.1"
                     /translation="MKRKKPVAVALRYDGNSPPKVTAKGEGLFAQQIINTAKEHGIPL
                     EQNPELTALLSQVRLNEEIPKALYIAVAQILAFLYYINGKHSKE"
     gene            complement(30514..31680)
                     /locus_tag="I9760_000261"
     CDS             complement(30514..31680)
                     /locus_tag="I9760_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HAT5042059.1"
                     /translation="MSVEMSKTSSARFPPGQEIKLAKPATELYIGQILKTVVVTTLSN
                     DQVLININGQNLNAKASHHFTPGELFEVKVIANHPETILEVQQKTPLPSLLQNALLQY
                     LPKQAPATNLLQTLTQLMAENNLSAPINQVMKTILGNVPVLTQLPNQLMQAISQSGVF
                     LESKLLKGKIPREIQSDFKGLCFKLLHRLGTDSKLPINLPNELESGFLQQAALPLPGA
                     IPQPLAKDTILNLIGLSTEAVHHILREQVTQALARITTNQINYLSQENQEAYLIMLDF
                     PLKTPDGIDVIPIMIKQHKAEPMQLSKWSISFALNLSHLGSIQATVSIYAKNIDIKVN
                     TQQSSTLQLLNDYHKDLEETLYESGLHLREWKLQVGLEENHIDATNLRLLDIKI"
     gene            31918..34155
                     /locus_tag="I9760_000262"
     CDS             31918..34155
                     /locus_tag="I9760_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042060.1"
                     /translation="MLDRASVVDEQFLKRVLQGDFPQLRSSMSPDTAELDKKTAIEFF
                     DSQIKSRLLDIIARQLKEKGLSFYTIGSSGHEGNAVLGQVFRASDMAFLHYRSGAFFL
                     QRAKQLPGIDGVKDILLSLVAAASDPISGGRHKVFGSVPLNIPPQTSTIASHLPKALG
                     AALSITRARELGLPSKLTPDSVILCSFGDASTNHATSQATLNACSWITQQNYPLPIVF
                     ICEDNGIGISVPTPSSWIKSSVSSRPGIHYIECDGLNIADVFAKAQEADYLARVKKQP
                     VFLHMRCVRLLGHAGSDIESQYNTQEEIEQREANDPLLHTAGILYREGWMSLQAMVEL
                     YQDNRALIEAKALEAIREPRMSSAEEIMASIVPRIDKKQKYSLPDDNRRAQVFAGAYN
                     QLTLKRNLCQQINFALTDLMMQYPNMLIFGEDVGKKGGVYRVTADLQARFGQRRVFDT
                     LLDETTIIGTAIGLAHNGFIPVPEIQFLAYLHNAEDQLRGEASTLSFFSSGQYQNPMV
                     LRIASLAYQKGFGGHFHNDNSIAVLRDLPGVIVACPSNGPDAAKMLRTCMRLAYEEGR
                     VVVFLEPIALYMTKDLYSPGDNGWLFEYPSPDEMISQGEVGVYGEGDTVILTYANGYY
                     LSRQAEKVLREAHNISVKIIDLRWLSPLPKDAILKEIAKAKRILIVDEGRQSGSISEG
                     LMTLLMEEASPRLKIKRITGKDCFIPLGTAWQYLLPSQESIIDAVIALQSDKREKESG
                     RLVVS"
     gene            34133..35290
                     /locus_tag="I9760_000263"
     CDS             34133..35290
                     /locus_tag="I9760_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT5042061.1"
                     /translation="MDDLLFLDEQLHDDERMIRDSVSRFVSNDVIPLMAESYEHAHFP
                     KELIKKSADLGLLGLTLPAEYGGAEASYVAYGLVCQELERGDSGLRSFVSVQSSLCMY
                     PIFKFGSEEQKKRYLPEMAAGNIIGCFGLTEPDSGSDPASMRTHAKKIEGGWILNGAK
                     MWITNAPIADIAIVWAKTEAGIRGFIVDTKSEGMSCPEIKLKMSLRASITGELVFDNV
                     FVPDENLLPKSEKGLGAALSCLSQARYGIAWGAMGAAMACFDIARDYLLERKQFAKPL
                     ASFQLIQKDLADMYTEIIKAQCLNLQIGRLKDQHRETPTMISLAKGNACREALKIARK
                     CRNLMGGNGISLEYHVIRHMLNLESVFTYEGTDNVHTLVLGRHITGINAFG"
     gene            35485..35898
                     /locus_tag="I9760_000264"
     CDS             35485..35898
                     /locus_tag="I9760_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042062.1"
                     /translation="MDIRCKTVLYKQLQEFNMINMKLKATLIACLSVLTLSGYANSSE
                     NKEAILQQCHDLASTVASLVSSQAKKTCAEKLVIASIHIDTAADWIVEDVHSAAKQEL
                     DNAIYSLQYAELNSCNRYIQISHSKLEAQRIKSLL"
     gene            36141..36395
                     /locus_tag="I9760_000265"
     CDS             36141..36395
                     /locus_tag="I9760_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042063.1"
                     /translation="MIELEPEPDTVLDPKDILTEEMKLEGSDNDMDHNQFTRGDEVDL
                     VGDGVPGYETTGQDGTDDEIEEQREARYTPTLNPNPKNVP"
     gene            36491..37249
                     /gene="mavM"
                     /locus_tag="I9760_000266"
     CDS             36491..37249
                     /gene="mavM"
                     /locus_tag="I9760_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavM"
                     /protein_id="HAT5042064.1"
                     /translation="MLVTPRFKIMVVVKKREETQKDERARAIENNPCSICRSLGLPFC
                     RGHGGGSGGGGSDSASDDKVDDNDHQLLAPKNPANVTNIEQLLLHSGLWHLPEDEDFV
                     FEFKDPLALVNMTLNMELGLLVLQGKRELGNEEQNALDELYEMIAKEFNLFKNSIKDP
                     QVPLETITLAREGNSLTIKIPSPKYYDLFVQQLMDKNLLPSNTLALQHQNQSVKYPEK
                     SKATMEKDLVGEEYKSSLPNPFDISRGPKLPDWI"
     gene            37545..37922
                     /locus_tag="I9760_000267"
     CDS             37545..37922
                     /locus_tag="I9760_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAT5042065.1"
                     /translation="MTVPEITLSRVYSPPEHKEGLWILVDRLWPRGIKKDSFLLDMWL
                     KDIAPSPALRKWFHQDSEHWHEFAKRYAQELKEKPELIEHVLALASTSPVTLFYAAKD
                     LQHNHALVLQKVLKSWPKIPEIK"
     gene            38036..38112
                     /locus_tag="I9760_000268"
     tRNA            38036..38112
                     /locus_tag="I9760_000268"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38070..38072,aa:Pro,seq:ggg)
     gene            38241..38477
                     /locus_tag="I9760_000269"
     CDS             38241..38477
                     /locus_tag="I9760_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HAT5042066.1"
                     /translation="MLADPIIYGKYRHYKNEKLYEVIGRARHSETDEEMIVYRALYHC
                     DQFGDNQLWVRPMQMFFENVIHHGKTVPRFQRIE"
     gene            38707..39255
                     /locus_tag="I9760_000270"
     CDS             38707..39255
                     /locus_tag="I9760_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAT5042067.1"
                     /translation="MRVGYARVSTHEQNLDLQLDELKKAGCEQIFTDKISGSVSERPG
                     LEKVENFLREGDVLVVWRLDRLGRSLKHLISYVSKLEEKGIGFHSVTEAINTETPSGK
                     FMFHVFGALSEFERNLIKERTLAGLEAARARGRQGGRPEKLSDEQKELVKTLYANKKH
                     SIQSICKMVGVGKTTLYKYINQ"
     gene            39277..40011
                     /locus_tag="I9760_000271"
     CDS             39277..40011
                     /locus_tag="I9760_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042068.1"
                     /translation="MFMSYYESKNNFESFVAKCIHTSRAFANIPSKSQQHFYSSVLFT
                     KMCVTAKTLLSVLPDREDGHWDYASAASLTRNIIECYLIFYYLCIDKISKSEWGCRWN
                     IFNLHDCKARISLYEKLGIKNGIDKFQETVKDLENRLNKNKYFLSLPDKQQKEFLKGK
                     KPLMVSQDDLVVKMGLNKNTFRGLYEFLSSQAHSFPLSFYKMKDDGRGRGVHCEVEEN
                     HTKVVIGYCIVFLEQAEKDMNVLFAQ"
BASE COUNT        11695 a         7302 c         8401 g        12813 t
ORIGIN      
        1 ttccttcttt agaaatttat ataaagctca aattaagggg aatatcagga tgaaatttaa
       61 gattgcaact tgtattgtta cgatgttatc cagttttata gtattttctt ccaactccaa
      121 tttttccgat aataatacaa acgatcttct tcatcttgac tggcgtgata ccagtgtgtc
      181 accagctgtg gatttttata cctatgccaa tggcaattgg aaaaagaata acccaatacc
      241 cgctgattat gcttcctggg gaagttttta tgttgtaaat gagaaagtac aaaatattat
      301 tcatcagatg ttaatcaatg catccaagaa tactcatgct aaacctggaa gtattgagca
      361 gaaagtgggg gatttttatt ttagcggtat ggatgttgat agcattgata agtcaggtat
      421 cacgcctcta aaaccagaat tcactaaaat agaagaaatt aagaatctca aagatttaca
      481 ggctgaaatt gcccatctgc acaaaatagg tgtagggcct ttgtttaatt ttggcagcat
      541 gcaagatttt aaggacagca cttccatgat aggtgctgtt atgcaagggg gattgggact
      601 cccagacaga gattattatc ttaaaaaaga agctaaattt aaacaggtaa gagaagctta
      661 tgttaaccat cttactaaaa tgtttgggct attgggtgac agtcctgaaa tggcggcaaa
      721 agaagctaaa attgtaatgg atattgaaac tcaattggct aaagcgtcca tgtcacaagt
      781 ggagcaacga gatccacacg ccatttatca tatgatgacc attactgaat tggataaatt
      841 gacacctaat ttttcctggt ctcaatactt gagcgctaga ggtcagggca aaattaaaaa
      901 aatcaatttt gctatgcctg atttcttcaa agcgatgaat gaattattac agtcagttag
      961 tttggatgaa tggaaaattt atctgcgttg gcatttaata gatgcattcg ctccttattt
     1021 atccaaaccg tttgtcgatc agaattttaa gatggtttct gcattaactg gtacacaaaa
     1081 actattacca aggtggaaaa gagtagttaa cactgaaaac ggtgcattgg gatttgcaat
     1141 cggagaattg tatgtagaga aatatttttc tcctgaatcc aaacaaaagg tcttggatat
     1201 actaaaaaat atcagagcag tattacaaga agatataaaa actttaagct ggatgtcacc
     1261 caagactcgt gatgcggcat tgaaaaagct agatttaatg gaagaacgag ttggttatcc
     1321 caccaaatgg tgggattatt ccagcttgaa aatagatcgc ggcccctacg tattgaatgt
     1381 tatcagagca aacgaattct tgatcaatcg agatctcgac aaaattggta aacctgtaga
     1441 tagaagtgaa tgggcgatga cacctcaaac gatcaacgcg tattatgatc cttcaatgaa
     1501 taacctcaat atacccgcag gtatattaca atctcccttt tttgatccaa aggctccgac
     1561 tgccataaat tatggagcaa tagggtatgt tataggtcat gaaatgacgc atggatttga
     1621 tgatcaaggt gctcaatttg atggttatgg aaatttgaaa aattggtggg cgccaaatga
     1681 tttatcaaaa ttcaaaaatg cgacacaatg cattgctgat caattctcaa aatatgtagt
     1741 agatggcgat ctgcatgtac agggacaatt ggttgttggc gaagcaactg cagatctggg
     1801 cggaatgatc ttggcatata aagcgtttca gcgttcaaaa gagtacaaga atgctaaaac
     1861 gatagaagga attacccctg atcaacaatt tttcttagga acagctcatg tttgggctat
     1921 gaatgtcagg ccggagcaac ttcgtaatca agtgacaaca gatccacatc cgcctgctgt
     1981 atacagagtc aatggtagct tggcaaatat gcctcaattt caagaggcat ttaatattcc
     2041 aaatggtagc cctatgataa gtaagaatcg ttgcatcata tggtaaagtg agttgatttg
     2101 cagtgattcc gggtacctta taaaaacgcg caatgacagc cgaatggatt acagatttag
     2161 ggatatgagt aatagaaagt tggaagttag ttaattaatt agagtttgct attcgaatca
     2221 ctcaatgagg gaggctgtta tgtgtcgatt cgttgcttat ttgggacgcc ctacgctatt
     2281 ggaaaatgtg ttggtaagac ctaagaattc aatcatcatg caaagtctgc atgcgcgcga
     2341 aaccaaatat cgaaccaatg gagatggttt tggtttggga tggtattcac cagaagttag
     2401 ctctgatcct gctttgttca cctccgttta tccagcctgg aacgatagaa atcttttaca
     2461 cttaacagca aaaattcagt ctccttgttt ttttgctcat gttcgctctg ccagtgcagg
     2521 aggagtaacc aattataact gccatccctt tatttatggc gattggatgt taatgcataa
     2581 tggggaaatt aatgatttta ttgatgtgaa aaggcatata aggcatttgt tggatgatga
     2641 catttaccat tgggttaaag gtcaaacaga ttctgagcat ttgtttggtt tgtttttgca
     2701 attagcaaaa ggaagagaca ttactcaact atcggtggct gctgatgtgc tgcaagaaac
     2761 tattaacaaa atattggaag taataggaca atttggccat agtggacctt cctattttaa
     2821 tatttgtctt acaaatggta aaaagattat cgctacccgc tattgcagtc atgagaagaa
     2881 aaaacctttg acgctgcatt atttgattgg gtatgtatta tctactcaag ggttatggat
     2941 aaaagaagaa ggtaatccag cttatgttgt agtctcttct gaaaggctaa atgatttgga
     3001 tgaaggatgg gaagatgtgc caccacaaca tatgatactg attgatgaaa ataaaaacat
     3061 tcagttgcgc cctttacaaa cattgggtta aacttgaatg attttaacaa taagagacaa
     3121 ttatggctaa agaagaaatg atttgtagaa taattagatt cattactcag aaaccaagta
     3181 ataaaaaaac ttgttgtgga cgttaatgag tttttctctt tctgaattac ttcactataa
     3241 aaatgaaaaa atagtgactc atttttgcta ttctcatcca gaatattcca tacaagaagg
     3301 gctatttctc tttgaggatt tattagcctg gatgtggctt aataagcaaa gaaggttgcg
     3361 ggggaaaaaa acctatcttt ttggaccatt attgatcctc gacgagatgt ggcattcttt
     3421 tattttgcat actcgggatt atattgattt ttcaatgaag tacttcggcg aatactttca
     3481 tcatgacgtt gaacctgtag gttttgagca tgtcttggat gaagaggaat tggccgatta
     3541 tctgcaagat tgctttcagt acctcaataa ggagtgggtt gaaaggagat tttcaatcgc
     3601 ctttgcggaa atggatgatt aagttcaata atagtgttga tgatgaaaat actttaacaa
     3661 tgaactatcc acaaaagtta ggaacactgg tttaaagcaa attgaataat ttccctcgaa
     3721 aaattatcag cgctccacaa atcattatgt tctttgtcgt caaaagaaac cattttttta
     3781 ggttcattgg cttcattaaa aacatttaag ccctcttgat acggtactat ctgatccagt
     3841 tttccatgca aaaccagaat gggggcatta atttttttca tacgggcaag tgaattatat
     3901 tgatcccagg gtttaaggaa gttcaatggg tagtgatact gggcaagcct ggttagtgat
     3961 gtaaagggtg attgaagtat aaccgcacaa accgggtatt ttgtggcaag gtgagttgct
     4021 acgccggtac ctatggattc cccatataga atcacgcgct ttgatggcac accatgttga
     4081 attaaaaatt caatggctgt ttctccatct tcataaagac ctttctcgcc aggtttgccc
     4141 ggattgcctc cataaccacg atattctaat aaaaatacgc ctaaaccagc gtcaataaat
     4201 tcacgaacca aaggcattct gtaaccaata tggcctgcat tcccatgcaa atagagtatt
     4261 gtagggcgat gcttgcttgc aggtttatac caggatttta aatgtaaatt atcttttgta
     4321 cgtaaggaaa ctactttcat atccgatgca ttgtaatctt ccaactttgg ggtatgtcta
     4381 ttagggaaat aaatgaggtg acgctgaaat aagtacatta gcgtaatgac aatccctata
     4441 ataactaggc cggttagcac aatttgtttc agcatgatca ccttaagaag gatttaaata
     4501 tattgtatta atttgattat aatgcacctt aatcgattgg aagccaatat cgatgatggg
     4561 cgaattgcct caaaagaaat gttagcgaaa gaaggaaata gtgaggctcc atttttatgg
     4621 ggaagttatc aatggagctt tacttaaaaa aattgtcgaa acgctataag tttgccatga
     4681 aaaaggctta attaattttt caagagttat ttgaattaag ccgaaactaa ctcacataaa
     4741 agcatgctat tcacagaatt taaaatggac acgtcctaaa tgcttacatt agggctattc
     4801 agtattcgct cttctgcttc tttaaatatt accatcacct ttttacgaga actggttttt
     4861 aagttcagtg cttttgttgt cttatcctga ccttcagcaa gaatttgcat ttctggggaa
     4921 gctaaaaact ccagtttttt ttcctccaat tgctccattg ttcttatctc agccaacgaa
     4981 tctctaagtt ctttcaggat cacttttttt agcccatcac cctttaaacc ctcatagatt
     5041 tcttgttttt tcgggctaaa aaagcgattc acccctacct catgcgaatg ttttttaaat
     5101 tttttatcct gagataactc ttctaatatt gtctcatgca gatcatgagc aagttttttt
     5161 ggcaatgata taagtccctc atggtaaaat cgaagcaacg tatggggatt atccgataaa
     5221 gattcgtctc tgttattttc ataccatttc tctataggga ctctccaatc aattctgtga
     5281 tcctcctcat cggtgggtgg atagatgaaa ttatagacat atagccattg atctttacca
     5341 taaacaagag acttcctgct gagttcatca actcttccct tgtcgtaccg aaagccacca
     5401 tcaagatcaa tagtctttac catttcttct gttgcaatca gattttgtgg actaatatga
     5461 taaggattat ttgccaaatc aggatgccag taccctgcat cattaagttc tttaagcgca
     5521 agaacaaatt cctcaatttt gggctggtcg atttttttat taatagggat cgcattgatt
     5581 ttaggcatgt agaaatacac gttttcttca tttttgatat tccagagtcc tagaacaaag
     5641 tgttcttgaa tgccatgata tttaaggaaa aattgctgtt ctataatatg gaggttaatt
     5701 tcttcataaa attctcttgg atgtctgcct cgagatactt tcatagcctt atcttctgtt
     5761 ttctcatata ccgttccttg ttctcctttt ccaagctcct tataatcctc ttcagtattt
     5821 ttaatcattt cactgttctg cgtatgctca ctcttgtgac ttttccacat taaaacataa
     5881 tccatgggtt catcattctc aaaacgaaca gcataaactt cgttattata acgatagatt
     5941 ttaaattctt tgtttggctc actcagataa tggggggtta ggtctgttcc attaacaatt
     6001 ttatttattt taggcatagc aagctctaac atattagttg aatatttgag cattatagtg
     6061 aaaaaaaaat cagaatacaa atgagatggt tgatgagtta ttcactttga gctaaatgca
     6121 gtaatgcctt tttcagtggc tcataagtac agtgttggct ctcactaata atggttgcct
     6181 tggctttttc agacagggta agagattttt tttgtgcagg gttttcgatt tgagaggagg
     6241 gagcaatgat ggtaatttta attgaagaca gctgataaag gccggcttct tttcttaatt
     6301 tatcccgtaa ttcaggaagt aaataacgta tttcggacgc aaaagcagca ttggttgtgg
     6361 ttaaaaccaa acacccttta ttaaaactac ctacttgaca tttagaaatg aaatgggaag
     6421 gaagaaatgc agaaatttta agagataact cttccaattg catagaatgc tggcaaattg
     6481 tactgagttg cttgtttagg cattgactga ttgatttcat aacgtccgtc ttaattgtta
     6541 ggtatctatt aaagagttag actatcgaaa agtaagttga tttatgtata gaatagcaaa
     6601 ttaacgttaa gtgcaatatc atattttttt gaggtatata agggtgatca atggctgaaa
     6661 atactacaga aagtaatgtc atttatttta ccagattgca ttggatcata ttttttggtc
     6721 ctttcgttgg attgggttta gtgatggcaa tttatattta tatcatccag ttgcgcgaag
     6781 tgagtctttt tttaggattt tttgcactga tctggatatt gattacctgg gtaacctatc
     6841 atttttcttc cattaccata aaaaggaagc aagttatttt acgtaccggt atcatagtaa
     6901 ggcaaacggt ggatatcccc ctgaataaaa ttgaaacgat agacatacgt caatccattt
     6961 taggcagtat tttaagttat ggtactttga taatcacagg aactggtgga actcgctatt
     7021 taattaatta tttatataaa ccgttaacct gcagacgcta cattgaacaa ttgatgaatg
     7081 aaccacacta aaatagttct cactaaaaaa agattattgt gatataactc tgaatttccc
     7141 aatttgcaaa cctttgtaca gaaataaagt gcaagggtgg gcatgtaatt agtattaatc
     7201 aacaatcatt ttatggaaat tgtagtgtga actatcgctc agatattgat ggcttaagag
     7261 caattgctat tttatttgtt ttattttttc atggaggctt aactcttttc ccatcagggt
     7321 ttattggagt tgatgttttc tttgtcattt caggctttct cattacaagt atcattgaaa
     7381 aatctttgca aaataatcgt ttttctttta tggactttta cagcaggcga ttatggagat
     7441 tacagcctgt ttttatttgt ttgattgtag tcacaacctt ggttaccctg ttttattatc
     7501 tgcccgatga cttgatgctt tttagtaaaa gtgcccgtaa aacatctatt tttacatcga
     7561 atgtgttttt taaaaatgtc acgagcgatt atttttctcc ggatagcaat caattgcctc
     7621 ttttgcatat gtggtcttta tccgtagaat ggcaatgtta ttttatcttg ccggttggaa
     7681 tctatttgct ttaccgattg gttggtccac agcatcgaat caagataacc tatttattaa
     7741 ccatcacttt tttcgcatta tccatgtatt actcttggag taatcctgca aaaacgtatt
     7801 atcaattttc cagtcgtatc tttgaatttt taattggttc ctgtgttgcc tcagcttcag
     7861 ggagtaaacg ctttttagcc aataagtatg tattaaattt tataactgtc ttggctgttc
     7921 tgacgctgtt ttatattgca atgcatcatg acattaatat aggttttccc aattggtata
     7981 ctttaatttt atgccttgca acggccatac tgattgccgc cggacaacaa gagccgcaac
     8041 ctatagtaac ttatgctctt tcattaagac ctctggtgtt cataggatta atttcctatt
     8101 ccttgtacat ttggcattgg cctgtattgg ctttaattcg ctatttaaac attgaagagt
     8161 ccaatttggt tctgttggtt acttttgggt tgatatcgat tatcgcttat ctttcctggc
     8221 gatttattga aaaaccagca agaaaattac atcgaatccg gttcagctat acagtggttt
     8281 gtttattaat tatgccggtt tttgtcactc acttgagtga ttatctgatt aaaaagaatg
     8341 cgggttatcc ttatcgcttc aatgaagcaa tccaggttta tgcggaatta aacaagtaca
     8401 aaaatccaca gaggcctttg tgtctggagc gcaaaaatac cgaagtcaac cctcagtgcg
     8461 ttctgggagc aaaaaactcg gatagtcgaa ccggctttat gataggcgat tcttattcca
     8521 atcagcattg gggatttatg gatacgttgg caaaggaggc caatgtatcc attttggctc
     8581 atgcaacggt tgcttgtctt gctttacctg acatatatca atttgattgg aatgttaaaa
     8641 ataagattta ccaggaatgc catgatcaaa cgaatcgcta ttttaatatg attaaaacca
     8701 accactatga ttatgttatt ttaggccaaa actggaatgg ttattttggt gatagaatta
     8761 ttaatcaatt gaatgacaat cgatctcaag agctttcttt aaagcgcatt gaaaaggctt
     8821 tggataaagc tctggagctt attaccactt caggagcgaa acctgtatta atcaaatcga
     8881 tagcactgtc taagggaaat ccttacaatt gtttttttga gcatatcaag ctgcataaaa
     8941 aatacaatcc cgagcggtgt gattttaacc tggatgtcag tgaacagatt tggttcgata
     9001 atttgtttgc aaaaatgcag cgaaaataca agacactggt cattattgac ccacgagtag
     9061 ctcagtgtcc gtcggggatg tgtaaggtag atatcaatgg tattccagta tttcgcgatt
     9121 caggacatat caccgattat gcttcctatc atttggccaa attatactta gagaagaata
     9181 acaatccctt agtgagttga gataatttgt tgggttaatg gattgcgaat aaaacaaaca
     9241 gatcattcgc aatccaatag cctgaggtac tcagggtgta aaaactggag taacaaagta
     9301 cctagtcttc cttggcagag taatcacaac cctcgcgagg acaaagaatt tgacgaccaa
     9361 attttttact ttcttttaag gttaagattg gccatgcaca tttagggcaa ggcaaatcaa
     9421 gcggttcatt ccacagagcg taatcgcatt ttgggtaatt tccacaggaa taaaagattt
     9481 tcccttttct tgatttccgc tgcagaattt ttgcctggct gcatttaggg catgtgacgc
     9541 cagtatcgga gggtttttct aaaggttcca tatgcttgca ttcaggataa ttgctgcacc
     9601 caataaattt tccatagcga ccggttttaa tatgcaaggc tccatgacac agagggcagc
     9661 ttctgccttc cacgacttca ggttctgatt tttctccttc tggattgctt atatcctgag
     9721 tgtaatcgca atctggatat cctgtgcagc caataaagcg ccctctcttg ccgagcctaa
     9781 tggacaatgg cttttgacat ttaggacatt tttcatctaa taattccgta gtaacatcct
     9841 ttctttgcac ttgttcatct atattctgaa tttgctggac gaaaggttgc cagaactctt
     9901 ccagaacagg gatccaatcc ttttctccac gcgcgatggc atccagggtg tcttcaaggc
     9961 cagcggtaaa ctgatagtcg acatagcgag taaaatagtt ggttagaaat cgattgacaa
    10021 ttctacccac gtcagtgggc aaaaaacgtt ttttttcaac taccacgtat tcacgctgtt
    10081 gcaaagtatg aatgatagag gcataagtag atggtctgcc tatatcgtat tcttcaagag
    10141 ctttaaccaa agttgcttca gaatatcttg gaggtggctc ggtaaaatgc tgattggttt
    10201 caatatccga tacttttatt ttttcaccta cattgaaagc aggtaaaatt ttatcttcat
    10261 tgtcttcgtc tttagaatca tctcgtcctt cttcatagac agaaaggaag ccagggaagg
    10321 ctattgttga accattggct ctgaaggtat tccctttacc acagctaaaa tccacgctca
    10381 ccgtatccag gatggcatca gccatctggg aggccactgt gcgtttccag attaagctat
    10441 agagtttcaa ttgatcgctt gtcagggaac cttgcaccat ttctggagtt cttttgatgg
    10501 aagttggtct aatggcttca tgtgcttcct gtgcgttttt ggatttagtt ttgtaaattc
    10561 tgggagaatt cgggcaatta tcccccttat aacgttgtgt gatatagtcg cgtatttcgt
    10621 caatcgcttc ctttgccagg ttgactgaat cagtacgcat gtaagtgatt aaacccaccg
    10681 ttcctgtgcc tatatcaata ccttcatata attgctgagc gaccatcatg gtttttcgtg
    10741 cggtgaaacc taattttctg gcggcttcct gttgcaaggt agaggtaata aaggggggag
    10801 atggcttacg tttacgctgt tttttatcaa tttgagcaac ggttaaaaag ccttgtgctt
    10861 gtgctatcag ttgctttttt atttcatggg cttgttcttg ttgagtgact gaaaactgtt
    10921 gaagtttctc ttcgttataa tgggtcagtc gtgcttcaaa ttcggtgctg gcatgagcgc
    10981 attttgcgat tattttccag tattcctgag cgatgaaacg ttcaatttcc tcttctctct
    11041 caacaattaa cctaagggca gggctttgta ctcgcccggc cgataatcct cgccgaatct
    11101 ttttccatag caaaggggac aggttaaagc caaccagata atcaagagcg cgtcgggctt
    11161 gttgagcatt caccaaatcc atggatatgg agcgaggatg attaattgca tcctggattg
    11221 ctgcttttgt aatttcatta aaaaagattc gatgaactga tttgtccttt aataggtttc
    11281 gttctttcat taattcaaaa atatgccacg agatagcttc tccctctcta tcagggtcag
    11341 ttgccagcag tagagagtct gattttttaa gagtttttgc tatcgtatca atgtgtctcg
    11401 catttttttc aataggaaca taagtcatgg aaaaatgttt ttcagggttt actgaccctt
    11461 tacgggcagg cagatcacgc acatgaccat aagacgctaa cacgtcatag tcatttccta
    11521 aatatttctg tattgtttta gctttcgcgg gcgactcgac gatcaccaag tgtttactca
    11581 tatcaaataa accttatcct tttgggttgt gtggctcact aacagttcat gaatgggttg
    11641 atatcagtac actcattaat tagtgaattg ttaattcatc ttctgtttgg tatagcacaa
    11701 gatcaagaaa agttaattgc tcttcattca aggaggatgc cagagcattt cttatagtcc
    11761 attttgtttc ttgcaaggtt acaatacgag attcagagaa ttgtagttga tttactattg
    11821 attcaaattg atcgatatca aggatattcc atagtttcat acgcattaga aattgatagc
    11881 tggcttttgt cagtttcatt tgctcatcgt aagtaaaaat acgtgttgat ttttcttgag
    11941 cagatttgat aaacaatgat tgctcttcag cacttaaaag atcttcttca agcaattgac
    12001 tttgggagtc ctgatcattg gatttatggc ttttttggag ttgagtaaga cttttttcaa
    12061 acaaattcaa taacatttca aataaattat ctttcatatt cacaccttat atagccaccg
    12121 ggcactgcca tcacagcccc tttcaattct agttcagcca gtccactcgt gacttgttcc
    12181 atgccatatc cgctacgatc aatgatttga tcgatagtcg tagtttcaaa accaatgaac
    12241 tttactaggt tttccttacc gctggcaagg gaaaaaattg gtttattaga agtgaactga
    12301 tggtgctcga tttttagctc atcaagaaca tcagccactg aagttactaa tttcgcaccc
    12361 tgttgcaata agtagtggca gcccctggct aacgggttat gaattgatcc gggaatagcc
    12421 aatacatctc tgttttgttc cagagccatt cttgctgtaa ttaaagaacc gcttttaatt
    12481 gctgcctcga taaccaaaat gcataatgat agaccgctta ttatacgatt tctgagtgga
    12541 aaatgtccag caattggtgg actttttaaa ggaaattcgc ttaataataa accattctct
    12601 gtaatttgct cagctaattt taaatgccgg cgcggataga tacaatcaat tccggtacct
    12661 aagaccgcta tcgttttgcc gcctccttcc agacagccta aatgagcatg agcatcaatt
    12721 cccaaagcta aaccactcac tatgcttacc ccatgcctgg aaatttccct ggagaatgcg
    12781 cgggcattct cgtttcctgt tacagaagca ttgcgacttc caacaatagc caggttgggt
    12841 tgtgtcatgg cggacagctc acctttagcg tataaaatga agggaggatc ggctatttct
    12901 ttgagcaatg cgggataata gggggagtcc caagttaaaa tatgatgatt ttctgatgaa
    12961 cttaaccagt tcaaatcagt ttttataaag cccggatcaa aattttttat ggtttgtgcc
    13021 aaccaggatg gcaagcccgc ttcttccaga tcaacatttg acagttggaa cataaggttt
    13081 aaatcaggcc atcttttttg cagttttaat acggttcggg ggccaatttt tttcatcctg
    13141 tttaaggcga ggaggggttg cagattattc atgggttcgt tactgtatct aataaatgaa
    13201 tagcacgcgt ggaccttact atcagggcat aacttgcttt tgtaaacaca cgaaacacca
    13261 tagcttcgcc aagacgttct tgcggtaagg taataggtct gagatgattt tggggatcat
    13321 tgacaatacg ttccttgcca tagatagcca aaacatctcc cggttttatc ccggaggttt
    13381 ctccaagatt aatgatgatg actcctccaa cagcctcttg tgtatttcca acaggcatat
    13441 tgtcaggcat ttcaataatg tatcctctga cttgcctggc aggggtttcg ggttcgaaat
    13501 acaattcaaa ttcagggtgg ttattaatca ataccttatc attgattttg atgccctgat
    13561 taatactggt caataaaacc gtagcgggct cgccgccagc aaccaattct ccataaccga
    13621 ccaaaactgc tttatagcct aacaattcat gggttattgg atcaaaataa tttctccctc
    13681 ccctgaagat ggagtatcct atagtgcctc cttgaggcat ttctttggag ggatgcaagc
    13741 ctctgacata aatttcatct ccctggcctc caagcatccg ctcactcatg agtgctacca
    13801 catacggtgc tcggtttagg acattaacat ccagaattaa cgattcatcc aaaaaaggtt
    13861 tgatatcgcc taatggtatt ggtggaacgg cgtcttcctg gggtgtcact cttatattgg
    13921 gggaaagttt gattgttccg ttagacagaa ccctgagata gggaatgttt tgataatact
    13981 ctaaagcgag aacggcacca ggatataacc tgtcgggatt tttaatctgg gggtttgcgc
    14041 gccataacgc tttccattcc caggggttat tcaaaaaacg gctggagata ctccataagg
    14101 tgtctccggg ttgcacaaca taacgagagg gggaatccgg tcttaggctt aaggcatgag
    14161 taatggatga tataatgaaa cagaaaatga ggagacaata tctcatgtat caatcccttt
    14221 agttgagcct tagcatcttg cagcattact gtatatcggg gataatatca cgattaacgt
    14281 gtggagaact agataaacaa tggcaattcg caagattctt tatttacctg atgagcggtt
    14341 aagaaaaata gctaagccgg tcgagacttt tgatgagagt ttacagacat taatcaacga
    14401 tatgtttgat acaatgtatg atgctcgggg agttgggctt gccgcaccac aaataggagt
    14461 cagcttgcgt ttatctgtca ttgatatagt gggagataaa aaggagcaga tcgttattgt
    14521 taatcctgaa atagtttcct ctcatggcga aaaagaattt gaagaaggct gtttgtctgt
    14581 cccaggggct tatgataccg ttgtcagagc tgaaaaagtg accgtgaagg cactggacag
    14641 gtttggcaaa ccgtttgaaa ttacgggtga agggctgctt gccgagtgtt tacaacatga
    14701 aattgaccat atgaatggca aattgtttgt cgatatgttg tctccattga agagaatgat
    14761 ggctcgccgg aaattagata agttcaagcg tttacaagct cgtaagccat gaatggttta
    14821 actgtcgttt ttgctggaac tccagaattt ggcttaccct gtctggatgc cttaattcaa
    14881 tcaaggcatc atctcaaggc tgtttatact cagcccgaca ggccggctgg ccgaggccgt
    14941 aaattacagg agtctcctgt aaaagaatgg gcaataaatc accagatacc agtttatcaa
    15001 ccgcttaatt ttaaaaatca agaagctgtc gatgaacttt ctgccttaaa gcccgatgta
    15061 atggtcgtga tagcctatgg attaatatta ccaaaagcag tgttggaaat tcctcgtctg
    15121 ggttgtatta atgtgcatgc ttctctgtta ccgcgctggc gtggcgcatc tccaatacaa
    15181 catgccattt tacatggtga tgctgaatcc ggagtgacca ttatgcaaat ggatgttgga
    15241 ttggatacag gccctatgtt atgcaaggcc gcctgtcctg tgacttcctc ggataccgct
    15301 ggtagtttgc atgataaatt agcaaaaatg tcagtaaaac cattattgga tgttttggag
    15361 gctttagcat ccaattcagc acaatttgaa ttacaaaaca atgaattagc gacttatgct
    15421 ggtaaaatta ataaagaaga ggcacgaatt aactggcatc agtctgcagt ggaaatagat
    15481 agaaaaattc gcgcatttaa tccatggcct gttgcctata cgctggctgg ggaattaatg
    15541 ctcagaattc accaggcaaa agcaactgat atcatgagta ccgagatgcc gggcatgata
    15601 ttaaatatcg ataaaaatgg aatgttggtc gctaccagtg ataatgcatt gttagtggaa
    15661 aagattcaat ttcccggagc aaagatcatt tcagtcaggg attggttaaa ttcgggtaaa
    15721 acacaattgc atactggttt aatgttgcaa tgaaagtaaa tgacagattc aacgccttaa
    15781 aaattttgac ggccttgata gaaagcagaa aaaacctgtc ctatttgatg tctccgcaag
    15841 agatttctcc catgacaaaa gaaatttgct atggattttg tcgtcattat tttcgtttac
    15901 aatccatagc tgatacttta atcaataagc gacctaaaga taatgagcta tggatagtct
    15961 tgttgatagg tatttatcaa ttgcagtata tgaataagcc ggattatgcc gtggtgaagg
    16021 agacagtgtc tttactgggg caggttaaaa aaagttgggc aaaaggactg gtaaatgccg
    16081 tgctaaggaa tttttgccgt cgaaaagacc atattctgga aaagctggca gcagagccgg
    16141 tttttatcta tggacagccg aattggttac ttcaacgcct gcaaaaagac tggccaaacg
    16201 attggcaatt catcgctgca gcgaatgata gacacccacc catgactttg agagtcaatg
    16261 tgttgaaaca ctcagttgat gagtatcttg atttactaaa aaaatccggg atagaggctt
    16321 ttaaacaccc tgtcgcacct gatggaatta ctttggcaat cccatgtgat gtgatgacgc
    16381 ttcctggttt tgcgcaaggc tctgtgtctg ttcaggatgc cgcgcctcaa ttagcagcgt
    16441 atttattgtc tttaaaaccc gggcagcgag tactggatgc ttgttgcgct cctggcggaa
    16501 aaacatgtca tattttggag aaagagcctg gtttggcttc ctgtgttgca ttggatttag
    16561 acccgcagcg gttacagcgg gtcagagata acttaaatcg attggatttg caagcaacgg
    16621 tggtgcaagg tgatgtattg gaacccagaa gctggtggga tggtcatcca tttgatcgta
    16681 ttttactgga tgctccctgt tccgctacag gagtgattag acgccactct gatatcaaat
    16741 tattacgaac ggatgaagag atttcagcaa tttctcaggt acaatccagt atgttgaatt
    16801 cattatggcc actgcttgca ccaggtggtt taatggttta tgccacatgc tctattatga
    16861 gtgatgaaaa tgagttgcaa atcgcccaat ttgttgcaga gcattcggat tgtcaagtga
    16921 gtgatggcaa atggtcctgg ggaaggtgga ctgggcatgg tcaacagata ttgcctgggg
    16981 agcatagcat ggatggcttt ttttacagtg tattgcgaaa ggatgaaaaa tgataattga
    17041 ttggcctgta attattgtct tgttttgttt atccattcct ggtgttttaa tcgtgatgca
    17101 tcgcctggta tattttctgc tagccgataa taccgagaga ttaaaaaaaa gatttagtcg
    17161 ttttgttgtg ctgcaaacct tatttatgac atttgtcatg agttttgctg gctccatcct
    17221 gtcttcacgt accgggctag gcgatcctct cctggaaggt cttttgcaag gcaaagccag
    17281 cctagattcc tttcaagtga ttttattacc tactttttta tatgcccttt tagggttggt
    17341 tattttttgt atactgtatt atgggttgtt tggttccata cttgatgaaa aaagtttcaa
    17401 gattatgtcg gaattgcggt ccaaaatagg acttgatggt tgtgtccttt atggaggggt
    17461 tgtagaagag gtaattgcca gatggggatt aatgaatgtg gtgtgctttt ttgctttatt
    17521 gtttacaagt caaattaatt cggaaattat aattatttca atttttttga gtgcatttat
    17581 ttttgctgta ggccaacttc cagcctatat tgcagcaggt tgcacaccat ccagacgatt
    17641 tctttactct tttgtattat tgagtctttc gcagtccata ctctttggtt ttttattttg
    17701 gcaatatggg ctgacctcag caattttagc gcacatgcta tttcatttgg gttgggacag
    17761 atatgaaaaa ttattttaat ttttatttcc tgttttgatc aagatgggag ttttttagca
    17821 tttatgtcct taaaatactg ttaaaaaaac agttatgtgt aaaaaaaatg taaaagtgcc
    17881 atctcttaat tttgtcttaa ttttgagcat ttattctgtc tttaatgaag atgataatca
    17941 aaggttgagg tatggccgcg caattagatc caagtagcga atttgctgct ttggttaaac
    18001 aattacaacg agaacccgat aatccgggat taaagcaagc cgtagtgaag cgcctgcctg
    18061 aaatgcagtt attagctaaa acgaattctt tggctttatt tcgtctggca caagtttatt
    18121 ctccttcatc atcacagcat aagcagatga ttttgcaatc agctgcccaa ggttgcacga
    18181 acgctatgtt atctgcttgt gagatattat tgaaatccgg agctgcaaat gacttgatta
    18241 ctgctgcaca ttatatgcgc ctcattcaaa gctccaagga ttcgtatatt attgggcttg
    18301 gcaagaaact gttagaaaaa tatcccgact tcgctgaaga gttaaaatcc aaagaagtac
    18361 cttatcagtc cactcttcga ttttttggag ttcaatctga aagtaacaag gaaaatgagg
    18421 aaaaaataat aaacagaccc actgtttaga ataagttttt tttcgtttca gggtttttct
    18481 ttgtggtcag ataaaccgga tacaattcca tttcaggcac tattgtgaaa caatttcata
    18541 tccttaagtt tcttgatttt ttcctatgca aacccgattt acttatttac ctgttacaag
    18601 cctgcaggca ggtcagtatc aacccaggca agattttaat gtaacggagt tacaggaatt
    18661 ggctcagtca atttcttccc agggtttaat tgaacctttg gtggtaagac ccatagcaaa
    18721 agagcgatat gaaattattg caggggaaag aaggtggcgt gcggccaaat tggcgggatt
    18781 aaatgaagtc ccttgtttga ttggagatta tagtgatcaa caggcatgtg ctctcacttt
    18841 aatcgaaaat attcaacgtc aggatttgaa tttaattgag gaagccactg gttataagcg
    18901 tttaattgaa gaattccatt atcatcagga cgagatcgca gcgttggtag gtaaatctcg
    18961 tagccatgtc gcaaatattc ttcgtctcct taccttgaca gataaggtta aacagttggt
    19021 tcgagacaaa gtgttatcct ttggacatgc tcgtgtattg gttggtttaa atcctgaaca
    19081 acaagaaatg tttgctcagc agattattgg agaggaatgg tctgtcagac acttagagca
    19141 agaaattaaa tcttataaaa ataaagatat aaatagccca aaaaacccca gaaaagacag
    19201 agatattgag cggttacaga ctatcctggc tgaacaagtg ggggctcctg tacaaattgt
    19261 aaacgatggc gaagacggag gttggttaaa agtaaaattt tttgataatg atacccttgc
    19321 agggcttttg gagcgattgg gcttgagata tgattagctt attggaacta actcagaaat
    19381 tgtcactatg tatcaaacaa ttcaacctga tttatattgt gaaattcaat gttaaggaaa
    19441 atatttaaat gaaaaggacg ttgactgggg cattcatgtg ggcagtatgg tcactgtcat
    19501 ctcatgcggc tagtatgcag ttcgaggtgg ataaattaat taatcggcta aaccctcatg
    19561 tgaacctggg gatagtagtg accgatctaa cctcgggcga gacgctatat aaacgcaatg
    19621 ccaatcgttt gtatattcct gccagcaaca tgaagctttt ttccgaggca gcggctctga
    19681 tggctttggg tccagactac caattcaaga atcaactcag tacgagcgcc aatcaattac
    19741 aacaaggtgt tttgcacgga aacttgtatt tacatctcag cggcgatcct tcgtttagca
    19801 gagaagattt aaagacgttg ctttcttcac tcaaggattg gaatatcaca accattcaag
    19861 gtaatgtgat cattgacagt agcctgatgt ccattcctgc ctatccccca ggctggctaa
    19921 cttcggattt atcttatagt tatggtgcgc caatagcacc tttgatggtt gattccaacc
    19981 gattaacaat cacggtcaac cccggggcta aagcaggcgc tcctgctatt gttgaagtag
    20041 atgatggcgg cggtaccatt aacctgaata atcaagcgac cacgaaggcg agtgagaaag
    20101 gatgtggtgt agggttttat ttggatcctg agaacaattt aacggtaaga ggctgtgttg
    20161 gattagggca atgggctgtt cagcaacgca tcgctataaa gaatcctttt gtttatgccc
    20221 aggggatgat tgtgagtgag cttgccaagt ctaacattaa attaaatggg caggttttgc
    20281 ttggaagagc tcctgctggt actttattaa ttgccactcg ctattcaaag ccaatttccc
    20341 aactgatggc agatacttta aagccttctg ataatttata tgctgatagt ttatacttgc
    20401 atgctgctgc caaaattaag ggtagtcctg tggattggaa gcaagcacag ccagtaatta
    20461 aaaacttttt gcagcaacaa acaggaatcg atttaaaaga ctcgaatttt acagatggtt
    20521 ctggattatc acgttataat ttggtaactc cagcgcaaac catggcgctt ttaaagttct
    20581 tgtatcaacg tttcccatta tcatacgaat acattgctgc tttacccatc tctggtcggg
    20641 atggtacttt gcaaaaacgg tttaaaaccc caaatcaaca aggatttgtg cgagccaaaa
    20701 ccgggacgat gacggggatg aacagtttat ccggttactt gtatactgct aatggccata
    20761 ctttggcttt tgctatgtat atcaatagat taccaggaaa accagctggg ccaggacgtc
    20821 cattattaga cgcattatgc acttatttct tgcagcaaag cccaacaagc agtcgactgg
    20881 cacgggtatt atctccccac agtcgcatta agttccaatt taatcctaca caaattgagt
    20941 tacagcgagt tcatcaagcc aaatggaggc gtttggaaac ggcagtgagg caggtcttga
    21001 gaggacagga tgtgaatgtg gtttaccggg gcaatgaatt aatcgtgaca gacaatcagt
    21061 ctaatgcgaa tagcgtttgg aaagctttac aatcaattgg caagaaatat tcattcgccg
    21121 tagctttgtc ttctaaagtg atgcctgtta caccatcggg aaaacctttg cttctttggg
    21181 tgcaagcacc tttatctgaa aataaggcag agagaacctg gataattcgg gaagcggtat
    21241 aatctgagtt tgataattaa aagaaagact agcctcatat tacaaaatgt aatgcggggc
    21301 tcttccaacg taacgttatc ttgagtcgtt tgggctgagg aggcatttat gctgtctcga
    21361 agccttgtat gagatttaat atttcgtgcc agggcttcga gacgatgctt atgcatctcc
    21421 tcagcctgaa cgaaccctgg atatagccag gctctcgtat aatccttatc tatttattgt
    21481 cgcgctgcaa acatgtctgt ggtcctgaac aactgatttt gaattgattt gagctgaagg
    21541 catcatcagg gattgagttt tgaatgtgct gttctgccca aaacacgatt taaatgatgt
    21601 gaagttatga taaggtcagt tgttatttcg aaccgtttgg gctgaggagg catttatgcc
    21661 gtctcgaagc cccttgtacg ggatttcata ttccgtgaca aggcttcgag gcgatgctta
    21721 tgtatctcct cagcctgaac agaccctgga tattggcagg ttctcgtaca atccttatcg
    21781 gtaaaactca tgttaaatag actgtttttt ctgcattcgg gctttgcagt attcaaggtg
    21841 attgtaacag tatttacgaa aactccaagg tcaaggcaaa aaatgttttt aatgcaagag
    21901 ttcagataaa ctaaatggcc gaattaaaaa catttttcaa caaagagatt gaggtttatc
    21961 gtaagtactg tgtaaaagag ctataattga gaactgctta atatcggtat aaattgattt
    22021 ggagtagtaa ttgaatttta gtattcgagt aattcttttg ttaacaggtt ttgtttcttt
    22081 agccttttct gcaccaaaat ttctaacaat ctctgatatt cattatggtg cagaaaatat
    22141 agcaaaggat gggcaggata ctggaaagga gtttttggat gtcacgttca aacgatttga
    22201 ggaattgtct caaaatgtag attttatttt acatttgggt gatctgccta ctcactcgtt
    22261 attttcgata cctaaaaaag aagaatacga acaagttgta tttcatggtt tgcatgaagc
    22321 gaatccgggt caaaagccca tgttttatat tacaggaaat aatgattcct tgcttggcaa
    22381 ttaccagcca ttttcttcag acaataagtc gccactcaat ttggctctgg attgggatgg
    22441 tgcttgtgct tattgtgatg ggttaattat tgatgatact catatgcgca aggatggcta
    22501 ttactccagt tatgtcatcc ccaataataa agagattatt ttaatagcac ttaattcggt
    22561 tcaatggacc aagacccctg tttttttacc aaagtaccct aatcaacaaa gggatgcctt
    22621 tgtacaatta ttttggcttg aacaacaatt aaaaaatcat cgtgcaaagc aattgttgtt
    22681 ggcgatgcat gttcctcctg gtacagccta taacggcaac cggttttggc atgacattta
    22741 tctggataga tttttgacgt tattagacaa atatcatcaa tcctatgatc aaatcaccat
    22801 actgttctca cactcccata tggaggaatt caggaaaatc agattatccg atggctcaac
    22861 tatttatgcg ttttctactc ccggaattag cagggcgcat cacaacaatc ccgggatgaa
    22921 aatttttgat ttagacaagc aaatgagggt cagaaattct actacttatt atacgtccga
    22981 tttgtatgag tggggtaatg agtattatca agccatgaat actcctgacg ccatttttcc
    23041 aaattgtcaa aataaaacga taccccagtg cctggacacc ttaagtccag agcaagtatg
    23101 caataatctg gaagtgggac tattttatgg ggtgaaaagt acgcgtgttc cctatcaggg
    23161 atgcattaaa acttaccaga ttaattaagt taggatttct ttgatcaggt aagtttaacg
    23221 agatgttcct tgttgtcttc aggttgtaaa tacgctgcga ctgctttttg taaggcagca
    23281 atatcatcct cgcttcttgt cgtcgccttg tttctaaaga aatcaattat tggattttgg
    23341 tgagttttta ttatttttga atggtcaggg tttaaggtaa atgcagagat taacccatag
    23401 atgttttcta ggtttccatg ttggcatttt atttcaaaaa taaaatgctt taataactca
    23461 cctttttcac gtcgttgatc atcagtggat tgacacatca attgtgctct tgaatggata
    23521 gcatttaaca tcaatacgat attttccgga agcttcttta ccggtttatc ggttttgacc
    23581 atggagtctg ttgtttttgc cagagcatca gcggtttctt tgtgcctatg ttttgcttga
    23641 agcggtggtt caaatcgaaa aactttatca tagtatttct ctttgaaacg ctcagctaac
    23701 caatcatgca ttgttgcagt cgggtgaaca tcatcccaga acatatatcc tttggctggt
    23761 gaaatatgtt ctttttgatc aacagggttt ttctgatttt ctttaaaaag atccgattca
    23821 acgtaagggg attttaactt gtctctatca aagccatatt gttcgggatt actgtaaact
    23881 tccttaaact gctcattaac atcaaagaca gagagattga caggaattcc caattctttg
    23941 tattttttga ttaattcaag agattttgtc tgcagttgtt gattaaagta ttcagtacaa
    24001 tcagatgcat tacgctgttc ttcagcattt tttctttgaa atcttggggt caatgagaga
    24061 ttgggcaaat tgaataaaac aaaatttctg tacccattat gaataagcgc ttcaatattt
    24121 tgaattcggt cgttaactgc tttctctgca gccgcacgag aaggcttgtc attggcggtg
    24181 atcaagtcgt tcgcgcctga ccactccaca actaatgttt cagatttttc aaggttggaa
    24241 atattgtatt ttttatcatc agccaataat tgagtccgtt ttcctcccaa agtagcgaga
    24301 atcaatcttg taaaaaacaa aaccaggtta aaggtaaagc tacaagagta gtcatgagaa
    24361 gttaatccac cttcgcaata gaagcgggca aatctttccc ctttaaacag aatatgattg
    24421 tcttcattta agctaaaaga gttttcattt tctcttcgtg ccttatcatt gatgagtaat
    24481 tcatcgccta tatccgcatt gtctcttgca ttattatgca atcttaattt tcttcggata
    24541 tgctctatct caaattgctc tgcagtggtc gcaccgacat aatcccccca aagaaaacca
    24601 ttagtgaatc gaccttttgg agatttgctt cttaatccac ttaaataact catggggata
    24661 aaacccagca gctcacgttt attaagcgtt cctctatcgg ataaactatc tcctaatact
    24721 acaaggtgtg tgattttagt tggtttttgg gccatgatat ttgcaagaaa aataacgtga
    24781 tcacattata cacaaaatga tttttaaatg gtatgttttg gactgatctt ctggtgaaat
    24841 ttttattgcg gaggacgtaa aatagctgaa tcaagtttgg tttaggaata ccctcaccta
    24901 atccacttgc aaaatgaacc aggatgaggg gagtttggct taaccaatta tattgatttt
    24961 atgatggtga tttgttcagg ccgtcattga ctcttcttct gattcattat cagcaagagc
    25021 acggatacgt tgtgcctcaa tagttaaaac cttgaagtaa tcataggaca tgtaaaaatt
    25081 acccttgtct ccaaattgct caccccatga gttacgtaaa gtaaataatc ctttgtgctc
    25141 ccggccattg tcatcaacag cgacggcatt gtcatcatat ccagttataa tcatttcatg
    25201 accgccaaaa aatgggcgaa gataaatatc acgagcaatt tcaggggtta gtacccaggt
    25261 atcgaattgt gttttattcg ttcctacggc acccataaca cctaaatcaa aatccagcaa
    25321 taataccgca aaagtaaccc gatctttttg attcaaagcg gtttttatat cattaatcgt
    25381 tttgttggtg tctgttctgt ctaatacagc ctgataaaca tccaggatgg gcgtgtaaat
    25441 aacaggatta gtctctggat cttcgctcat ttcatggaat tgctcaagcg acatggcatg
    25501 atcaggggta ggttgctcgt tgtctgggta ttcagttagt ccaccacatc ccaggttttt
    25561 ttgtttttct ttgctgacta tcccaaaatt ttccatttga cttaagacta tgcgccctaa
    25621 tgagccatcc caaccacttg gtgaatgacc gtttttctcc agatacagcc ctagttgtaa
    25681 ttggcacaat tggctgatgt agtcaccttt gtttaaagca gcatctatgg ccgcagtcgt
    25741 tgcaaaagtg acgcaagttc caaagcttcc ctggttcaga acaggtacgt tattcatacc
    25801 taattctact ttggatggga gagctgttga tgtggttatg gtattcggtt ttttgagtgt
    25861 aatgtctgtt tttctcgcaa gtgctttgat agcagaattt gacaactgca ttttaagcaa
    25921 tttgatttgt tgtatggcgg gttttgtgtt gagtgtttta ttttgcgggg cttttaatgt
    25981 ttggtttaac gttcctacta ctttgatatc ttgagcaaat aaattcccgc tcaatgccaa
    26041 agttaaaaaa agtaattttg aaacacgcat ctatcattct ccgttagtga tatggacttg
    26101 aaatggctgg caaggataac tgatgatgaa ataatattca atatatgttt aatataccca
    26161 ctaaataaaa aataaattca tattgggttt taattgtctt ccagggttgg gttgagtcgt
    26221 ttcatccagg acatgaataa gtgtgtcaga gtgagttctg atgttaacta tttattcgac
    26281 tggaaaattg aaataggtgt ctggataagg ttcatttttc agggtgaaat gccaccattc
    26341 atagtaataa ggtctaaaac caaagcgttt catgagcttt tgtagtaatt cgcgattggc
    26401 tttctggttt ttactcaact tggaataatc aatgttggct gacacatcca gacaatcaaa
    26461 acgagttcct gtatcgatcg aatcatcatt cagatagttt ggtgttttat cgtagcaacg
    26521 tgttaaagga tgattagatt ttttttctcg agaggctgtt agtttcacca gcgttaagtc
    26581 aagggtactg cctcgcgtgt ggccagaggt tagagcaata taaccacggt caaacaaatc
    26641 ctgttttatt tcacggggat aaaattcagg tttcattcga tgatctttgg cattgcggct
    26701 ccacttataa aaataatcca cagccctttg tggcctgtag caatcatata cttttagcgt
    26761 atatcctttg ggtttgatgt attcttccgc tttttttaat tgttgcgcgg tttgtctggt
    26821 gactatacat actccgcgtt tgtaacctgg gataggattt cctatgaaat tattatgggt
    26881 ggcatatctt atgtcttcaa tgatgtcagg tgctacatcg tgtaaataaa caaagccttt
    26941 gggaagcgaa aaactggccg tgcttatgag ataaagaaaa aaaagagcgc tgatttcttt
    27001 cattgttaga ttgttaaact cataaccctt agaagtttac tggcgtgggg gtgctttcgc
    27061 ttcgattatc atcctccatc gctgctgtta agtcaacttt cgaggtcata tctttgggtt
    27121 tttcaaactc ttgttttgga gtggattgtc tttccaagac agactctcgc gtcacaccta
    27181 attgttgatg aagtttggca ttcaccgtta aatgatcctt ttgttcggtt ttgtgttgtg
    27241 gtgaattttc tgatgccgct ttcataccct cgcgaacaat ggatttaatc tttccttctt
    27301 ctatcttgtt attaatagca tcaatagcgg taccaaaaag ataagtggcc gcaaaagtaa
    27361 cgccagcaac aatggcagca ccggctgcca caccaattcc caaggcggcc acagccaagc
    27421 ctgccggagg gaatagcaaa gtaagggcga caaatccacc agctgctagt aacccaagcc
    27481 cgagacctgc tttaattttt gtacccatat ttttggtaaa agcgttttca ggccccattt
    27541 cttttatttt ggcaaaatta gcgaccttgc caaaaaggcc attatcagcg aaattggctt
    27601 tatcctgaat ttcaaacttt ccgccggaaa cacccgtatt ggcagtatga tgaagggcct
    27661 gtatagtagt aagttcccga tcaagttgtt ctacattact gacttcttta ccatgtgtcc
    27721 ttcgatgatc cagaaattca ccagtgtata acccgtcctc actcattgct gtccataaag
    27781 cattcccaac aattttttgc gccaatcttt ggccacgatc attattactc ttacatcctt
    27841 tcgtattggc gtaatccagc tcgcaggaga gagaagccat taaggtttgc agcttgaaat
    27901 tatttttgcc atgatgccag gtgttctcat aatagagctc ttgagcttgc gtgaggtaag
    27961 cacatgaatt gtaaaagtgc tgcagtctgg atttggtttc aggatctgtt tctttattct
    28021 ggattacagg gcttatttgt tctttaagtt cttgtaaagc cttattgatt tgccctttgt
    28081 gttctgtgta ataagccttt cttaaggcat cgatttgatt atgtagttca taaatggctg
    28141 attcttgttt tttaatgctt ttttcatagc catcaacctg atttttcaat tgatccagtt
    28201 tggcttgatc ccatttccct tcgcctttct gatattcttc gaaatagctg aataatgctt
    28261 ctcgataggc tcctttcacc tcttttaagg cattttcttt ctcagtgatt tgttgattaa
    28321 ggtcgctgac gtctgggcct tttaataatg tgtgtaatgc gcctgctttg tcataatcac
    28381 ccaggttttg tgccactttg tctaaatttc tgatgacgtc atcagtcatt tgatttacaa
    28441 aacgcgcatt ttgatttttt gtgacactat tgtcagagag cggattgagc gctccaacct
    28501 gtctaaacca attcactccc caggaactca tacgcgcctg ataaccaacc gctgtttcca
    28561 cttctgtatt ggagccggtt tgaatggtga tttttgcttt ggagccatca aaagcatcca
    28621 tcatttgccg tacgtattca aattgttctt tttgattttc ggccgctacc ctttgtccgg
    28681 ctgttacaac ttgtgtataa cattccgtga cagataatgg attatgttct acatacagct
    28741 ttattaattg cgctcgtgct ttctccgcat caggaggaag ctcatcaggg cggtactctt
    28801 caggaatttt attccaaagg tcttcattgg tcattcggtc aatttgacgc tgtgccaggg
    28861 tttggatgac ttcttcctgg ttgattaagg tgaccgcttt gatagccagc tttttatagt
    28921 catcagcacc cttaaaatca gaatagggca tgcgtgcacc aggaccagaa aaactgtcat
    28981 ggtgaatagt cacggtgcca tcagaatgtc ttgtcccggc aatcatgcgc gccatactgc
    29041 tgttgcccag aaccaggtca gaacgtttat gacttgtagt gtcgtgatct ctgttgactt
    29101 cagattcatc cttgcccatt agtctttgtt ggcgtttggt gtaccttcca cacggaatgt
    29161 acataataaa ttgttcttcg ccttctttag agttgggcgc tttaaagcga tgtatcaagg
    29221 tatcgcgatt atgactgcga atattatgcc cttctttatc cagtgctttt aatttatgac
    29281 ctgaggcttt tgcgacagcc tgtgtgaatt gctgtaaact gccttcgact ttggccaact
    29341 gttccagaaa gtctttattg gcagagcttt ttttacttga aaacaaatcc gcttttgctt
    29401 tgtcggaagg aatggtttct gaaatggact ttttgaaagt ttttgccatt tcatcaatgg
    29461 cattttttct caaatccttc tcacctttct tatctttgtc tatcagagat ttggcagcgg
    29521 ctacaagatt gtctatataa ttattcgcct cttggatcac atcaggctgc cctgtaacag
    29581 gtctgccatg tttatcatat tgactgttca ccaccacgac ttgtttccca tccagcatcg
    29641 ggtctttgaa tacctgtctt tcgccgggat aaccgtaggc aagatgctga ttttgagaag
    29701 gaacgggttc cagatcactt aataaatgcg tgacataggt ttctatattt tcagtgattc
    29761 gtggcatggt ttattccata tcataacatt tattttatta tagctggcaa acattaacgt
    29821 aagattaata agtttttttg acacaaattt agcttgcatt gagtgattgc gtagggcttc
    29881 acttaaagac tggactgaaa aatagaattt agtactattc tcagaaaata gtgaagcctg
    29941 ataatttcct agtaaaactc aatagtggtg gtagtcacat ccaaaggctc cggatcatcg
    30001 acatctaaaa tgacaggctc aaccactgga gcggcaactg tcacttgttg gtattcaact
    30061 acttctgctg tagtacaaca gcaacctgca gttgttataa taacaacgat cagcattatc
    30121 catttcatag cgaaacctct cgtcataact taaccaaatt gtagattatt tgttcagata
    30181 ttgccaatgt tttatggatg cctttgcgtg tttgttcatc tgaagaagtt caaattcgtt
    30241 tgaaatgggt ttattctttg gagtgctttc cattgatgta ataaaggaat gccaaaattt
    30301 gggctacggc aatataaagt gctttgggta tttcctcatt gaggcgaact tgagacaaga
    30361 gcgctgttaa ttcgggattt tgttcgagag gaatgccatg ttctttggct gtattgataa
    30421 tttgctgagc aaataaacct tctcctttag cggttacttt gggaggagaa ttgccatcat
    30481 aacgtaatgc aacagcgacg ggtttctttc ttttcatatt tttatatcca ataaacgaag
    30541 gtttgtggca tcgatatgat tttcttccaa cccaacttgc agcttccatt ctcttaagtg
    30601 aagacccgat tcatagagcg tttcttccaa atccttatgg taatcattga ggagttgcaa
    30661 tgttgacgat tgctgagtat taaccttgat atcgatgttt ttagcgtaaa tcgaaaccgt
    30721 agcttgtatg cttcctaaat gggataaatt caaagcgaac gatattgacc atttagacag
    30781 ttgcataggt tctgccttgt gttgtttgat cataataggg attacatcaa tgccatcggg
    30841 ggtttttaaa ggaaaatcaa gcatgattag ataagcttct tgattctcct gcgataaata
    30901 attaatttga tttgtcgtga ttcgcgctaa tgcctgtgtt acctgctcac gtaaaatatg
    30961 gtgtaccgcc tctgttgata gtccaataag attcaatatc gtgtctttag ccagtggctg
    31021 agggatagct cctggtaaag gaagggctgc ttgctgaaga aaacctgatt ccaattcgtt
    31081 cggcagattg attgggagtt tactgtctgt gcctaaacgg tgtaataatt taaaacacaa
    31141 ccctttaaaa tccgattgta tttctcgggg tatctttcct tttaacaatt tggattctaa
    31201 gaaaacacca ctttgactaa tcgcttgcat aagttgatta ggtaattgag tcaaaacagg
    31261 gacgttgcct aaaatggttt tcataacctg attgatggga gccgataaat tattctctgc
    31321 catcaattga gtcagtgtct gtaaaagatt ggtagctggt gcttgttttg gtaaatactg
    31381 aagtaacgca ttttgcaata gagatggaag cggtgttttt tgttgcactt caaggatggt
    31441 ttcggggtgg ttcgctatga cttttacttc aaataactct cccggtgtaa aatgatgaga
    31501 tgcttttgcg ttgagatttt gcccattgat attgataaga acctgatcat tagacagagt
    31561 tgtgacaaca accgttttca gaatttgtcc tatataaagt tctgtcgccg gttttgctaa
    31621 tttgatttct tgtccaggag ggaatctggc actggaggtt ttgctcatct cgaccgacat
    31681 acaccatcct tatcatcagt tagactgctt tttcttatcg tcgtccccca ctggactgtt
    31741 tcgtaagttg tctaataagt atatactcat cgtcttaatt atcggcagaa ttatgaattt
    31801 ctttatagtt cgttgggaaa ggataccagg catggatata agagtaatca ttacacaaaa
    31861 tgtaattgaa atagccttat tcatctggtc attgcgcaat ttaaacaggg tgtggtgatg
    31921 ttagatagag caagtgtagt ggatgaacaa tttctgaaaa gagtgttgca aggggatttc
    31981 ccacaattaa gaagcagcat gagccccgat acagcagagt tggacaagaa aactgctata
    32041 gaattttttg actcacaaat taaatcccgg cttcttgata ttattgcgag acaattaaag
    32101 gaaaaaggcc tctcttttta tacgatagga agtagtggtc atgaaggcaa tgcggttttg
    32161 ggtcaggttt ttagagcgag tgatatggcg tttttgcact atcgcagcgg tgcctttttc
    32221 ttgcaacgag caaagcaatt acctggaatc gatggagtaa aggatatttt attgtcatta
    32281 gtggctgcgg caagtgatcc tatctcaggg ggcaggcata aagtctttgg cagcgtccct
    32341 ttaaatatcc cgccgcaaac atcgactatc gcatctcatt taccaaaagc tttaggcgct
    32401 gctttatcaa ttaccagagc gcgagagtta ggattgccaa gtaaactgac gcctgattcc
    32461 gtgatacttt gttcctttgg tgatgcttca accaaccatg caacgagcca agccacattg
    32521 aatgcttgct cctggataac ccagcaaaac tatccactcc ccattgtgtt catttgtgaa
    32581 gataatggaa tcggtatttc agtacctact ccatccagtt ggattaagtc atccgtttct
    32641 tctcgtccag gaatacatta cattgagtgt gatggattaa acattgcgga tgtttttgcc
    32701 aaagcgcagg aagcggacta tttggcaaga gtaaaaaaac aacccgtgtt cttgcacatg
    32761 cgttgtgtcc gtttgttggg ccatgccggt tctgatattg aatcgcaata caacactcag
    32821 gaagaaattg agcaacgaga agcaaacgac cctttattgc atacagcagg aattttgtac
    32881 agagaaggtt ggatgagttt acaggcaatg gttgagcttt atcaggacaa ccgggcttta
    32941 attgaagcca aggcactcga ggcaatcaga gaacccagaa tgagttctgc tgaagaaata
    33001 atggcttcaa ttgtcccacg aattgataaa aagcaaaaat actcattgcc agatgataat
    33061 aggcgagcgc aagtatttgc cggagcttat aatcaactca ccctaaagcg taatttatgc
    33121 cagcagatca attttgcctt gaccgatctg atgatgcagt atcctaatat gttgattttt
    33181 ggagaagacg taggaaagaa aggtggagtt tatcgagtta cagctgattt acaggctcgt
    33241 tttgggcaac gcagggtttt tgataccctt ctcgatgaaa cgaccattat aggtactgct
    33301 attggtttgg cacataatgg ctttattcct gttccggaaa ttcagttctt agcttatctg
    33361 cataatgccg aagatcaatt acgtggagaa gcatccactt tatctttttt ttcaagtggc
    33421 caatatcaaa accctatggt acttcgaatc gcctctcttg cttatcagaa gggatttggc
    33481 ggtcattttc ataacgataa ttcgatagcc gttttacgcg atttgccagg ggttatagtg
    33541 gcttgcccct ctaatggccc ggacgcagca aaaatgttga gaacatgtat gcgccttgcc
    33601 tatgaggaag ggcgggttgt cgtgttttta gagcccattg ccctctacat gaccaaggat
    33661 ttgtatagcc cgggagataa tgggtggtta tttgaatacc cgtcacctga tgaaatgatt
    33721 tctcaaggag aggttggcgt atatggggag ggggatactg ttattttaac atatgccaat
    33781 ggatattatt tatctcgcca ggcggaaaag gtcttgcgtg aagcacataa tatttctgtt
    33841 aagatcattg atttgcgctg gctcagcccc ttgccaaaag acgccatttt aaaagaaatt
    33901 gccaaggcaa aacgtatttt aattgttgat gagggtagac aaagcggctc aattagtgaa
    33961 ggattgatga cattattgat ggaagaggct tcgccacggt taaaaataaa gcggattacg
    34021 ggcaaagatt gcttcatacc attaggaact gcttggcagt atttattgcc aagtcaggaa
    34081 agtattatag acgcggtaat cgcattacag tcagataaaa gggagaagga aagtggacga
    34141 cttgttgttt cttgatgaac aattacatga tgacgagcgc atgatacgag atagtgtgtc
    34201 tcgttttgtt agcaatgacg taataccttt aatggctgaa tcttatgagc acgcccattt
    34261 cccaaaagag ctgataaaaa aatctgctga tctggggttg ttgggcttaa ctttacctgc
    34321 ggaatatggg ggtgcagaag cttcttatgt tgcctatggt ttagtatgcc aggaattgga
    34381 gcgaggtgat agcggtttgc gcagctttgt ttctgtgcaa agttctttgt gtatgtatcc
    34441 tatttttaaa tttggcagtg aagagcaaaa aaaacgctac ctgccagaga tggctgcggg
    34501 gaatattata ggttgctttg gattaactga gccagactca ggatctgacc cggccagcat
    34561 gcgtactcat gccaaaaaaa ttgaaggtgg ctggattttg aatggtgcta aaatgtggat
    34621 caccaatgcc cctatcgcag acatcgcaat agtgtgggca aaaacagagg cgggaattcg
    34681 tggttttatt gtcgatacaa aatctgaagg catgagttgt cctgaaatta agttaaagat
    34741 gtctttacga gcctccatta ccggggagct ggtttttgat aatgtttttg ttccagacga
    34801 gaatttattg ccgaaaagtg aaaaagggct tggagctgcg ttaagttgct tgagtcaggc
    34861 gcgttatggt atcgcatggg gagcaatggg cgctgccatg gcttgctttg atatcgctcg
    34921 ggattatttg ctggaacgca agcagtttgc caaacctttg gcttcgtttc agttgattca
    34981 aaaagatttg gctgatatgt atacagaaat tattaaagca caatgcctta atttgcagat
    35041 tgggcgttta aaggatcagc atcgtgagac accgactatg atttctctgg ctaaggggaa
    35101 tgcttgccgt gaagcattga aaattgcgag aaaatgtagg aatctaatgg gcgggaatgg
    35161 aattagtctt gagtatcatg tcattcggca tatgctgaat ctggagtcgg tcttcactta
    35221 tgaaggtacc gataatgtgc atactttggt cttgggaaga catataacgg gaattaatgc
    35281 ttttggttaa ggatttttct gcttttatcg agttagttct gaataccaca agataactat
    35341 tcttaatccg taatgattac ggttccagtt cctttttctt gcagaaagag gaactgttta
    35401 agcctttttg attttagagt tgagcgattg cccgccagat tatggtgctg aattaggaag
    35461 gtatggtcct ggcatggtta ttttatggat attcgatgta aaacggtttt atacaaacaa
    35521 ttacaggagt ttaatatgat taacatgaag ttaaaagcaa ctttgatagc ctgtttgtcc
    35581 gttctaactt taagcggtta tgcgaattca tcagaaaata aagaagccat tttacaacag
    35641 tgtcatgact tggcttcaac agtagcgtct ttggtttcca gccaggctaa aaaaacctgt
    35701 gcagaaaaat tggttattgc ttcaatacac attgatacag cggcggactg gattgtagag
    35761 gatgttcatt ctgcagcaaa acaagaatta gataatgcca tttattcttt gcaatatgct
    35821 gagctgaata gttgtaatcg ctatatacag atatctcatt ccaaactgga agcacaaaga
    35881 ataaaaagcc tattatagta atagaaaata atggttatat tttttgaaaa aaggtctctt
    35941 tgcttattgg gcaatgagac tttatgtcca actgaagcag cagtttagcc tgtcattaac
    36001 ttcatatcac ccgatggttt tttaagtctt ttcccagcaa ggggcagaag gtattcggta
    36061 aactaatcct tgccaaattt cttatctttc tatccctgct acacttaaat atagagaaga
    36121 ttatatttaa gggcagcaat atgattgagt tagagccaga accagacaca gtcctggatc
    36181 ctaaagatat tttgactgag gaaatgaagt tggaagggtc agataatgat atggatcata
    36241 accaattcac ccgtggtgat gaagtggatt tagtcggtga tggtgttccc ggatatgaaa
    36301 caacaggaca agatggaaca gatgatgaaa ttgaagagca aagagaagct cgctatactc
    36361 ctaccttgaa ccctaatccc aaaaatgtgc cttgatctcc gtaatgaggt tcaaagtgta
    36421 gtttattgta aattttttgc aaaattcacc aagttgccaa atgaaacatt cattttaaat
    36481 tatcatttgc ctgttagtaa ctccaaggtt taaaataatg gttgtggtaa agaaaagaga
    36541 agaaacgcaa aaagatgaac gtgcacgtgc aattgaaaat aatccctgca gtatctgtcg
    36601 ttcattagga ttaccttttt gtcgtggtca tggcggcggt tctgggggtg gcggatcgga
    36661 tagcgcttct gacgacaaag tggatgataa tgatcatcaa ttgctcgcac caaagaatcc
    36721 tgccaatgtt acaaacatag aacaactact attgcatagc ggcctatggc atttgccaga
    36781 ggatgaagat tttgtttttg aatttaaaga cccccttgct ttagtcaata tgactctgaa
    36841 catggagctt ggtttattgg tgttgcaagg caagcgcgag cttggcaatg aagagcagaa
    36901 tgctctggat gagttgtatg agatgattgc gaaggaattt aaccttttta aaaattcaat
    36961 aaaagaccca caggttcctc tggaaacaat tacgcttgcc agagagggta atagtttgac
    37021 gataaagata cccagcccta aatattatga tttgtttgtg caacaattga tggataaaaa
    37081 tttgttaccc agcaatacac tggctttgca acatcaaaac caatcagtaa aatatccgga
    37141 aaaatcgaaa gcgacaatgg aaaaagattt ggtaggggaa gaatataaat catccctgcc
    37201 aaatccattt gatattagcc gaggccctaa attacctgat tggatataat ttttcccagg
    37261 caaggatgag cggaaaaatt tctccatcat gctattctta attaaagtaa gttatggata
    37321 aggattatat aagaagcagt tgatctccag gagtcgttca ggctgaggga tgcataagca
    37381 tcgtctcgaa gccttggcac gaaacattaa atttcataca aggcttcgag acagcattaa
    37441 tgcctcctca gtccgaacga ttagagataa ccccctatct tatccatata atcaaagaga
    37501 ttaaaatggc cctgaaattt ttccaatgat acggatgaca taatatgacg gtacccgaaa
    37561 ttactttatc cagagtgtac tctccaccgg aacacaaaga agggctatgg atacttgtag
    37621 accgattatg gcctcgggga ataaaaaaag acagttttct tctggatatg tggctgaaag
    37681 acatagctcc aagtccggct ttgaggaaat ggtttcatca agacagtgag cattggcatg
    37741 agtttgcaaa gcggtatgct caggagttaa aagagaaacc cgaattgata gaacacgttt
    37801 tggcgttagc gagcacttca ccagtcactt tgttttatgc ggcaaaagat cttcaacata
    37861 atcatgcgct ggtacttcag aaagttctaa aatcgtggcc aaaaatacca gaaatcaagt
    37921 agggcctatt tgagcgaaat aaaactcaga caagccatga acttatactt cactcatgat
    37981 agtaaaccat aacccttgtt attttagcct tatttagata tcatgcctaa ccaatcgggg
    38041 cgtagcgcag cctggtagcg cactagcatg gggtgctagt ggtcgaaggt tcaaatcctt
    38101 ccgtcccgac caagcccagt aagggtttta taaaattatc cataccccgt cagaaatatt
    38161 tggtaccagt tttggtaccg atgcaatagt agtataaacc agtcagtgaa aactaatggc
    38221 tattaatcac aagagtgtaa atgttagctg atccaattat ctatggaaaa tacaggcatt
    38281 ataaaaacga aaaattatat gaagtcattg gccgagcgcg tcatagtgaa acagatgaag
    38341 agatgatagt gtatagggca ctttatcatt gtgatcaatt tggtgataac cagctttggg
    38401 ttcgccctat gcaaatgttt tttgagaatg tcattcatca tggaaaaaca gttcctcgat
    38461 ttcaacgaat agaatagtat taatttaatt acttatgcta gctaattgag ttgctgaaga
    38521 ataatagtat taaggcaaaa cgaacccatt tgtcgttatg attcgtaaaa tgatgggttt
    38581 gtggacgccc gaaattgatg ctcaaaaatt tgtaattcac tgcaaaaacc agttcgaaaa
    38641 ctctatacat taatcaaagt tcattttaat ctattctttt agtctttctg aacaaaggga
    38701 ataataatga gagtagggta tgccagagtt tccacacatg aacaaaattt agacttgcaa
    38761 cttgatgagt taaagaaagc aggatgtgaa caaattttta cggataaaat tagtggttca
    38821 gtatccgaac gccccggatt agagaaagtc gaaaattttc ttagagaagg agatgtttta
    38881 gttgtttggc gattggatag gttagggcga tctctcaaac accttatttc ttatgtaagt
    38941 aagttggaag aaaagggaat tggttttcat agtgtaacag aagctattaa tactgaaact
    39001 ccttctggaa aatttatgtt tcatgtattt ggtgcccttt ctgagtttga acgtaattta
    39061 ataaaagagc gtactcttgc tggacttgag gctgcaagag ctaggggtcg gcaaggggga
    39121 cgaccagaaa aactcagtga tgagcagaag gagctggtaa aaacactgta cgctaataaa
    39181 aagcattcaa ttcaatctat ctgcaagatg gtcggtgttg ggaagactac actttacaaa
    39241 tatattaacc agtgagattt ttaatattga ataaggatgt ttatgagcta ttatgaatca
    39301 aaaaataatt ttgaaagttt cgtcgcaaaa tgtatacata cctcacgtgc tttcgcaaat
    39361 attccttcta aatcgcaaca gcatttttat tcttctgtat tatttacaaa aatgtgtgta
    39421 actgcaaaaa ctctattatc agtacttcct gatagagaag atggacattg ggattatgca
    39481 tcagctgcgt ctttaactag aaatataata gagtgctacc tgatttttta ttacttatgt
    39541 attgataaaa taagtaaaag tgaatggggt tgtcgttgga atatttttaa tttacatgat
    39601 tgtaaagcaa gaataagtct ttatgagaaa ttaggtataa aaaatggcat tgataaattt
    39661 caagaaactg taaaagattt ggaaaatcgt ttaaataaaa acaaatattt cctctcattg
    39721 cctgataagc agcaaaaaga atttttgaaa ggaaaaaagc ccttaatggt aagccaggat
    39781 gatcttgtgg ttaaaatggg tttgaataaa aatacattta gaggattata tgagttttta
    39841 tcatctcaag ctcactcatt tccgttaagt ttctataaga tgaaagatga tggtcgtgga
    39901 aggggagtac actgtgaggt tgaggaaaac catactaaag tagtcatagg ttactgcatt
    39961 gtctttctag aacaagcaga aaaagatatg aacgttttat ttgctcagta attgaattat
    40021 tgatcccaaa actcatataa ggtaggcagg aaattacata atatggaccc agtattagaa
    40081 tattatcaaa gaataatctt tagaaatcag caaattgaaa aacacatccc taataatttt
    40141 agaaaaatta tagaggcaaa aagcaaacaa gaagtggaag caattataaa aaatatcatt
    40201 atacaagagg a
//
LOCUS       DACTJE010000007        39896 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.007, whole genome shotgun sequence.
ACCESSION   DACTJE010000007 DACTJE010000000
VERSION     DACTJE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 39896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39896
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.007"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(157..1362)
                     /locus_tag="I9760_000272"
     CDS             complement(157..1362)
                     /locus_tag="I9760_000272"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAT5042069.1"
                     /translation="MIVQDSVCSELMRGCEEILPVPELEKKLQKGIPLKIKAGFDPTA
                     PDLHLGHTVLLNKLRQFQQFGHEVIFLIGDFTAMIGDPTGKNVTRMPLSQETVLENAK
                     TYQHQVFKILDPDKTTVAFNSQWLNKFNAVDLIRLAATHTVARMLERDDFNKRYTTGQ
                     PIAIHEFLYPLLQGYDSVALKADVELGGTDQKFNLLMGRELQKHYGFEPQVVMMTPLI
                     EGLDGVKKMSKSLDNYIGINETPEQIFGKIMSVSDELMWRYIDLLSFKTGKEIQQLKQ
                     SVLEGKNPRDVKIDFAKEIVARFHDQTQAELAHNNFIERFQKGNIPEDLEELSLVIVE
                     PIALAQLLKQIDLTASTSESIRMVKQGAVKVDGDKISDPSLKLPIGKSYIIQVGKRRI
                     AKLSIQQAD"
     gene            1619..3052
                     /locus_tag="I9760_000273"
     CDS             1619..3052
                     /locus_tag="I9760_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAT5042070.1"
                     /translation="MDKRPNIPKRKSGNSSKLLAFFALVFAFSLPYLLVKNFSHPKRS
                     SYADQTDLYPELSDELSRDIPAEASTEIPHEKPDIKSATKPMQTKQDQQDKPINTSHV
                     KKDNEWQIVNPRSGDSMATIFHRLGLTAQNLNDVIKNNPHAKMLTRIKPNQKLQFLIK
                     KNKLEKLIVPVNTIQTLTVYRAGKIYKTKIDSKKTTTQNRYVTATVQGSLYTTAQRFN
                     IPSKLVRQMTTIFNKEIDFSRSLRSGDQFSIIYDAYYIEDKMVGVGDILVVTYTNRGK
                     TYQAIRHTNAHGEQDYFTPQGTSFKKAFTRYPIKFSHISSTFALSRYHPILHYRRAHK
                     GIDLAAPIGTPIHATGDGVITIIDRHNGYGNMIKIKHDKTYSTVYGHLLKFQKGLSKG
                     SRVKRGQVIGYVGQTGLATGPHCHYELHVNNQPRNPTTISLPTAAPVPAREMAAFKAK
                     ASTLLARLKLFEQAQLASKGKKKINVG"
     gene            complement(3183..3716)
                     /locus_tag="I9760_000274"
     CDS             complement(3183..3716)
                     /locus_tag="I9760_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HAT5042071.1"
                     /translation="MNGFANHLSNWLFLTFIVGIPLYAAIKKVNVFDAFIIGAKQGFD
                     VSVKLIPYLVAMMVAIGMLRASGFFSLMSQILSPALTMIGMPSELLPLALIRPFSGSA
                     STGVMAELIHKYGGDSFIAKTAATMMGSTETTFYVIAVYFGSVGIRRTRHAIPAGLLA
                     DLAGIIASVLICRYLFT"
     gene            complement(3713..4318)
                     /locus_tag="I9760_000275"
     CDS             complement(3713..4318)
                     /locus_tag="I9760_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HAT5042072.1"
                     /translation="MLNLIWLGMILISVVVGVIEGRIDEVVRAVTDSAKLGFELALGL
                     AGIMTFWLGIMSVATESGLVSILGKVLKPVLKRLFPDIPVEHPAMGAMVMNIAANMLG
                     LANAATPFGLQAMKELQSLNQNLKVATNSMCTFLAINTSSVQLIPVTAIAFLAANGST
                     NPSSVVFTSLIATTVSTLVAIVAVKQLAKLPRFKIERTDSL"
     gene            4388..5458
                     /locus_tag="I9760_000276"
     CDS             4388..5458
                     /locus_tag="I9760_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HAT5042073.1"
                     /translation="MKHTYNPFRYLIYFAFFITSLSSYSSQTTNIKEQTDTGLAPPAP
                     SVTTNSAAIEEQANSSIRKLYHTLNTMPNTSMADRISQISAYFKGTKYILGSLGEGPN
                     ARYDQFPRYRVDGFDCDTYVNTVLSLALANSLESFQECLKHTRYKNGKRSYINRNHFT
                     SIDWNNYNQKRGLLKDITFSIRNEKKQPVALYANALINKPQWYNHKTIDTIRLQKQDK
                     NEQEKRLVELKAKGKKLETSLSNVPYIPFTALFSENKPNLHLFSQIPNGAVIEIIRPN
                     WDLRQQIGTELDISHLGFAIWINNELFFRQASSQYGKVVDVSLIDYLDKARSSPTIKG
                     INIQIVLPEKPVSNGCQLFDIR"
     gene            complement(5702..5995)
                     /locus_tag="I9760_000277"
     CDS             complement(5702..5995)
                     /locus_tag="I9760_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HAT5042074.1"
                     /translation="MTQYNFERWSEMAKKIQEPFQAIAELNVKTLQGLSYLKPDELAQ
                     VKKPEEMLEKQINLAVENGHKALDYMQKSFQIIEKAMLSLVQEAKKTTAEVKK"
     gene            complement(6202..6600)
                     /locus_tag="I9760_000278"
     CDS             complement(6202..6600)
                     /locus_tag="I9760_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate biosynthesis repressor
                     PhaR"
                     /protein_id="HAT5042075.1"
                     /translation="MTRLIKKYKNRRLYDTEISQYITVEQLQKYVVDGIDFKIEDSET
                     GKDLTNSVLLQIIVEMEAGPTQFLSSEILRQIISLANHPMHVSFKEMMEQMFHAMDKP
                     LQNNPYQQATEVWNKQMQQMVHQWQKMFKV"
     gene            complement(6730..7476)
                     /gene="phbB"
                     /locus_tag="I9760_000279"
     CDS             complement(6730..7476)
                     /gene="phbB"
                     /locus_tag="I9760_000279"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HAT5042076.1"
                     /translation="MLNNNVVLITGGTGDIGTAVAKQLDSTFKHVIALDLVSYDEGEA
                     WLHDLKTQGYKNINFRHMDVTDYEQCAEVIGLIIEEFGGIDVLINNAGITRDAVFTKM
                     TKRQWDEVLRVNLDGMFNVTKQVVESMKAQESGRIVNVSSVNAQKGQFSQANYAASKA
                     GVYGFTKSLAQELMIKNITVNSISPGYVNTRLMKGIRPDILEGIINLIPAKRLAEPQE
                     IAWAIEFLISEKSRYITGANLSVNGGLHMY"
     gene            complement(7655..8395)
                     /gene="phbB"
                     /locus_tag="I9760_000280"
     CDS             complement(7655..8395)
                     /gene="phbB"
                     /locus_tag="I9760_000280"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HAT5042077.1"
                     /translation="MDKMIAIVTGGTGGIGSAISQRLADSYQVVACYYKDGRHEEAKK
                     WQDEQKQLGYDIDIVYGDIAQYSDCEKITSLVMERYGRIDVLVNNAGITKDCSLRKMT
                     PQQWQQVLDANLTSVFNMTRNVVPVMLERGYGRIVSISSINGRKGQFGQCNYASTKAA
                     LFGFTKSLALEVASKGITVNTVSPGYIETPMLAALKEDVLNSIISSIPVGRLGYPKEI
                     ADAVAFLASPDSGFITGANLDVNGGQYM"
     gene            8661..9059
                     /locus_tag="I9760_000281"
     CDS             8661..9059
                     /locus_tag="I9760_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042078.1"
                     /translation="MFNKFLVTTLSVVTLTLSPYGFAKDDSCEVQVAVSTPPVQFDQS
                     VAFNVTNDDGISKSIILQGGSSPKTIGKLICSPLPYTISATQYFTPTGLTNKHAQPVG
                     QCVLKAGNVILNGSNNSVAVVFPNDFICHH"
     gene            complement(9100..10287)
                     /locus_tag="I9760_000282"
     CDS             complement(9100..10287)
                     /locus_tag="I9760_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HAT5042079.1"
                     /translation="MVFGFLNLSFWGYVLATFILTQITIASVTIYLHRNQTHRALTLH
                     PVVSHFFRFWLWLTTGMVTADWVAIHRKHHATTDVEGDPHSPVVEGIRKVFWQGAELY
                     REARKDRDMVAKYSHGTPTDWIERNVYSRHSSKGILLMLLLDLFFFGIPGMTVWAIQM
                     MWIPLFAAGVINGIGHYWGYRNFECPDAATNIIPWGFWIGGEELHNNHHTFASSAKFS
                     VKWWEFDIGWMYIRILSLFGLAKVKKLPPKLAMDEGKFHVDLDTVRAVISNRFQVMSY
                     YYKNVIRPILKHEKNTSIETKEDKKLFQRAGRLLRREDKLLSTKAKTRLQKLLDAREQ
                     LRVVYSYKQSLQNVWLKTATTQKELVEALQQWCRQAEESGLDVLKQFAQQLKGYVPVY
                     TRE"
     gene            10468..10698
                     /locus_tag="I9760_000283"
     CDS             10468..10698
                     /locus_tag="I9760_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042080.1"
                     /translation="MSDKVQKLIENIVPFIVIGVGIAIVVALLFMFFYVAIWGVIIGG
                     ILWLGALAKEYFFPSKSSQKEQGRIIEHDDKK"
     gene            10698..11522
                     /gene="mutM"
                     /locus_tag="I9760_000284"
     CDS             10698..11522
                     /gene="mutM"
                     /locus_tag="I9760_000284"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAT5042081.1"
                     /translation="MPELPEVETTKQGIKPHLEGRIITTAQVRNRKLRLPVPLNLNEL
                     CEGKHITAITRRGKYILLHMDKGYILIHLGMSGHLRIVSQTANPQKHDHVDLHINNGL
                     ALRFCDPRRFGLFIYIDENPYQHPLLAHLGPEPLSDDFNSEYLLRKAANKSQSIKSFI
                     MDSQIVVGIGNIYAAESLFLAKIHPNTSAKKITTEEFNSLTGHIKKILESAIEAGGTT
                     LRDFYSSDGKPGYFRFALKVYGRKNLPCLVCENKIETVVIAGRHSAFCPHCQPIIT"
     gene            11597..12217
                     /locus_tag="I9760_000285"
     CDS             11597..12217
                     /locus_tag="I9760_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042082.1"
                     /translation="MDIKKLKKAASDFLERYPDGFCHPEMIEMGKKHKMDKMVALAQG
                     SFARQNFKNPDEITNSMLKIVTQSSLISIFEKPKFRDFIKRTPPNDKVLIAKGLKELL
                     YGNEQEGFEELLFQFQREKLAKWTLMTVFQAYFNPEVDVFIKPTTTKNIIKELNLDIV
                     YNPTPSWEFYESYRNIINQMKTKIEKSLAPSNAAFCGFLMMSLDDR"
     gene            complement(12275..12345)
                     /locus_tag="I9760_000286"
     tRNA            complement(12275..12345)
                     /locus_tag="I9760_000286"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12311..12313),aa:Gly,seq:ccc)
     gene            12657..13724
                     /gene="dinB"
                     /locus_tag="I9760_000287"
     CDS             12657..13724
                     /gene="dinB"
                     /locus_tag="I9760_000287"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAT5042083.1"
                     /translation="MNPIRKIIHIDMDCFYAAIEMRDFPELANKPIAVGGDAKRRGVI
                     ATCNYAARQFGIRSAMPTAHALKLCRELILRPVRMDVYQKESQYIRSLLTEYTDLVEP
                     LSLDEAYLDVTESTQCQGSATWIAEEIRARIYQTRQLTASAGIAPNKSLAKIASDWHK
                     PNGQMVIRPEDVSAFVLDLPVRKLFGVGPKMEEKLGALNIKTCADLQRYSVEYLLQKF
                     GTMGQRLYELARGIDNRPVNPERIRKSISVEETYPKDLPNSEACLAVLPDLMARLEAR
                     IQRAGKISGIHNLFVKLKFNDFQQTTIERVMDKLDLIVLRQLIQEGFARRGMPVRLLG
                     IGIKLKQENTYQSIQLPLLDL"
     gene            14063..14383
                     /gene="sugE"
                     /locus_tag="I9760_000288"
     CDS             14063..14383
                     /gene="sugE"
                     /locus_tag="I9760_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HAT5042084.1"
                     /translation="MLLPWLYLIIAGLLEICWAVGLKYTDGFSKWQPSLFTVTTLIIS
                     MFLLAKAAQTLPIGTAYAVWVGIGSLGTAIMGILLFQEHLSVFRLLYLVLMLISIFGL
                     KYTA"
     gene            14452..15183
                     /locus_tag="I9760_000289"
     CDS             14452..15183
                     /locus_tag="I9760_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAT5042085.1"
                     /translation="MKISRLKTFWIITRLFYYTGVACTRSIFKYLFSTPTRPWVDKVI
                     HHWVDQLLNAVKVEYKVINPHNVQPQPGKATILMCNHSSAYDIPLGFKAFPNNSIRML
                     AKRELSKIPLLGKGMAAAEFPFIDRKNRFQAIKDLEFAQQLMESGIILWIAPEGTRSK
                     DGKLAPFKKGGFITAIQAKATIIPIGIRGAFDILPARTFNIHLNQKAEIHIGEPIDAA
                     QYTIENKEELIEKTHQSIKKLVGEN"
     gene            15392..17608
                     /locus_tag="I9760_000290"
     CDS             15392..17608
                     /locus_tag="I9760_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT5042086.1"
                     /translation="MSINKIEEDRDTSSDLEMKTKMYLVHRMDKAGPLTVKDVHILIN
                     KLSKDLQKNQLTLIDCQLIINLVESRFTLVKQATRHLQFFLNKLDGKSINPILIPLSN
                     KPFWQNEPHPFDHYRSTDQLPEKADIVIIGAGLTGASTAYHLIDAVKKRNLTVVLLDK
                     GNPATEASGRNGGNFELLPENSVGIYSGLVSERFRFLKRCYPHVHPSILQIESEYHAS
                     LVFQFALKNRNRFKEIARKERIYCDLAAKGWLYIAHTEEEEQAICDEVTLAAQHGERI
                     YIWSRKKIREQFGINSKFIGRFIPDDGTYNPFKYTCCLIKAAIKSGIKLYTFVKVQQI
                     KSMDTEYHQLKTNMGLLKARQVIVATNAFTSELLPELSAIKPHQSQIAITESTPDYCK
                     GRLITSEDGPLYLNQPRVRSKNGLVPLLIGAGDDRPMKNPYYRKRSKKVHDLIVQQRD
                     KYFPDLKGKPFSTEWVGALAFTPDQLPAIGYLSPGIIIVMCCNGYGGSYTTAAGFAAA
                     EIALTGKNPPWLPADVFSPKRLLSDKPLFLEDSDSLWRIGKTLCTTLNNLNQLLAESL
                     SYQSMYQPYSTISSLPHGHQEKTCNLDPLRVMHQLAIFRTFAYQELEMLIRYTSPVCL
                     NKGEILFKQGDAGHSCFILIQGKINLYYEHAEGFTSMVAELEAGSVFGQMALFLENKR
                     MATCQATENCSMLEIMVEPCKSLFIQQQALGIKLLRLLNQGVIDALHKLNKRLKYT"
     gene            complement(17706..19430)
                     /gene="ggt"
                     /locus_tag="I9760_000291"
     CDS             complement(17706..19430)
                     /gene="ggt"
                     /locus_tag="I9760_000291"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAT5042087.1"
                     /translation="MKYNYKWLSCLFFLICNIYQVNAKTFPQHPPGYAVASAHPLATH
                     AGLEILAAGGNAFDAAVAVAAALAVVEPYHSGLGGGGFWLLHQEKEHLNLFIDGREVA
                     PLAASKDMYLAPDGQVIPGLSLNGGLSAAIPGQPAALVYIAKNYGRLPLSKTLAPAIK
                     LAEEGFPVDPQFRYFSTLDDRLENLKKYPDTAAIFLKNDKPFEIGEKLVQKDLAKTLR
                     LIAKYGNAGFYSGETASLLVQGVNAAGGIWSLDDLAQYKIKVRPPLISAYHNMLIITA
                     PLPSAGGVALVTMLNILSKFPLSSFPKLKWVHYVVESMRLAYWQRDQFLGDPDFIKIP
                     LDKLISSDNAKQLSRLISPDKAIDSKVLQGSVPIGKHSNTSHISIIDTEGNRVAATMT
                     INYIFGSSVVAKGTGVLLNDEMDDFSTKVGEQNVFGLVGSERNAIEPRKRPLSSMAPT
                     FLEMPGRVAILGTPGGSRIPTMVLLASLLFYDGYGAISMVSAMRFHHQYLPDVLQFEP
                     DTFPSSIQQGLQAMGYHLMPLKQYYGDMQAITWDKESNVLTAASDPRHIGLAAAISNM
                     AYGYGVNY"
     gene            complement(19420..19926)
                     /gene="coaD"
                     /locus_tag="I9760_000292"
     CDS             complement(19420..19926)
                     /gene="coaD"
                     /locus_tag="I9760_000292"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAT5042088.1"
                     /translation="MQMVINEMKQKAIYPGTFDPVTNGHIDIITRASTIFPELIVAVA
                     SNKNKRPYLSWETRISLLEESVGHLTGVRVVGFDNLLIDFVLEQSAGIILRGLRAVSD
                     FEYEFQLAGMNRKLSKKVETLFLTPAEHLMYISSTLVREIAALNGDISQFVPPNVVRE
                     LKKRQNEI"
     gene            19989..20588
                     /gene="lolB"
                     /locus_tag="I9760_000293"
     CDS             19989..20588
                     /gene="lolB"
                     /locus_tag="I9760_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HAT5042089.1"
                     /translation="MNAIKRLTIILICFLTACAPPRPSEEPPANREIPLEERKAETAT
                     VSSWEIKGALAAKTKAKGWSATMNWVQQGANSYQIRLMGPLGGGTVLISKKGNTITFQ
                     DGSKITTSTNADELLLKQTGIRLPVNNLYYWVRGLPAPGGVQSEKHDQFNHLIQLKQN
                     GYTIDFTKYTSVKGIDLPSMIRLEGNGVMVKVVIKNWVV"
     gene            20716..20790
                     /locus_tag="I9760_000294"
     tRNA            20716..20790
                     /locus_tag="I9760_000294"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20748..20750,aa:Gln,seq:ttg)
     gene            20970..21917
                     /locus_tag="I9760_000295"
     CDS             20970..21917
                     /locus_tag="I9760_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAT5042090.1"
                     /translation="MSTMMIFTGNANPELALKISSHLQIPIGKATVGTFSDGETMVEI
                     LENVRGKDVFVLQSTCAPANNNLMELLIMADALRRSSAGRITAVVPYFGYARQDRRVR
                     SARVPITAKVVADMMASVGICRVLTVDLHADQIQGFFYMPVDNVYSTPVLLEDITKQK
                     LNNIMIVSPDVGGVVRARAVAKRLNDAELSIIDKRRSGPNKSEVMHIIGEPANKNCII
                     VDDIVDTAGTLCTAAQELKKNGAKSVRAYITHPVLSGPAVNNIKHSGLDEVVVTDTIP
                     LSAEAQNCEKIRVVSLADMLAQAIKRVNVEESVSSMFAE"
     gene            complement(21979..22260)
                     /locus_tag="I9760_000296"
     CDS             complement(21979..22260)
                     /locus_tag="I9760_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAT5042091.1"
                     /translation="MTTISNDAGDTTASLAESVTQSVQKYFSELKGTDPVDLYQFVLE
                     EIETPLFRAVMEHCKYNQSRAAIMLGISRGTLRTKLRRYFDDKYVGTRD"
     gene            complement(22450..23907)
                     /locus_tag="I9760_000297"
     CDS             complement(22450..23907)
                     /locus_tag="I9760_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAT5042092.1"
                     /translation="MKKLTGLVIILAVLILGGYYGMGILTERTVKKTVEVINQSNGLF
                     ADIQQYKRGWFCSDALVKWRLHVPERVVKDDDGKLQTTPAQDYQMEMPIKIYHGPIIY
                     ANKQLRFGMGFAQTVFPFPEKYNQQFNELFSQDSEKPQLDLSIFVNYLNKSTVELSIP
                     SFKLTAKDGNGKFNWMGMDSTTSMSSNLDKVEGEIEFDGAEFSKDDTKLSLGKVISEY
                     DLHKTTTGLYLGQASFSLPSLAVVVKDQKMFEISELTLSSSSDIDDNLFSTHFSAALK
                     SLFANGQNYGPGEVEMSLRNLDAEVLAKINEQANAMQRGTDQERQQALMAMLPELPKL
                     FSKGAEFEISKLSLKLPQGMIDGNLLITLPKGDNSNPFELIQKTQGNAKLKMPIEVVK
                     QLMQQSIMQQMAKQPEMQQALIQQLQSSQGQSNQAEPTPEQIAAMQVDKQINELEQSG
                     LITVDGKDYVIEMSLSEGKFTVNGKPFDPSMMKFQ"
     gene            complement(24135..25418)
                     /locus_tag="I9760_000298"
     CDS             complement(24135..25418)
                     /locus_tag="I9760_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042093.1"
                     /translation="MGISKVEIYKRKNYLGFAWLVWGLAAAFYFSDYLARVAPGVMHR
                     YLQMDFGINEAGFGILTASFYVPYILMQIPVGLTVDRLSIRWLLTIMSLVTAFGCCVF
                     GLADGLLTASIGRMLIGFSAAFAFICSLRLATSWFPPTMLGLLSGLTQALGMLGAAAG
                     EAPVSFLVSNVGWRHSMLIIAFLFIALSGLLYQFVQDKPGEHRNEIRSVNRISILDSL
                     KIILSNKQTWLNALYAGFLFGPTAVIGEAIGPAYLQFGRGLGAHAAAFATGLIFIGWG
                     ISGPLSGWISDKMGRRKPLMIISAVCGVILSSLFVFIPEMSQTTAYILFFVFGLTNTG
                     VAISYAVSTEIHDRSVVGTSIAFTNMTSIFVGALFQPLVGRIIDIVSGPRAYNVETLL
                     LSDFQAGLKLLPLCSLVALILAFTVKETYCKPIRH"
     gene            26067..26456
                     /locus_tag="I9760_000299"
     CDS             26067..26456
                     /locus_tag="I9760_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HAT5042094.1"
                     /translation="MNKLNIAIKEAFESSGSSKEANKVYLEFIKANFIIPIESNSDDS
                     EPRVLFLEENNHVFLPVFTDMKYLDAWASEISQHIKLLKLSGVDLLKGLGEKVTVCLD
                     IGSELYKEFNPSEIERMRSMVLKLFKN"
     gene            complement(26527..26898)
                     /locus_tag="I9760_000300"
     CDS             complement(26527..26898)
                     /locus_tag="I9760_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmission trait enhancer LetE"
                     /protein_id="HAT5042095.1"
                     /translation="MNDTTSLLSHLRLRFNIEHPSYEECYIFGYECALAEVPEEDNPF
                     REGSQEAEQWLEGWWAGIYGEKPLFDLNNIEEDILLDKTTAANDQQYHHKNSFLSLVF
                     EIGGAIAASAIVGYQLLELVA"
     gene            complement(27511..28158)
                     /gene="pdxH"
                     /locus_tag="I9760_000301"
     CDS             complement(27511..28158)
                     /gene="pdxH"
                     /locus_tag="I9760_000301"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAT5042096.1"
                     /translation="MSKFRSLADIRRDYGELQLSEESAENDPISQFKLWFDDVLLNEK
                     NDPTAMVLSTVDEKGYPDSRVVLLKGLENGNFIFYTNYQSAKAMQIQKNPYAALNFYW
                     PQMARQVRVRGRVKKISSEQSDAYFSSRPLKSQFSAIVSPQSQEILDRISLEDALNQL
                     IEEYGQKPVVRPENWGGYMIIPDEIEFWQGRDNRLHDRIHYYRHGHEWTHRRLAP"
     gene            complement(28581..29456)
                     /gene="sucD"
                     /locus_tag="I9760_000302"
     CDS             complement(28581..29456)
                     /gene="sucD"
                     /locus_tag="I9760_000302"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAT5042097.1"
                     /translation="MSVLVDKHTKVLCQGFTGKQGTYHTEQALAYGTKMVGGVTPGKG
                     GQMHLGLPVFNTMREAVEETQADASVIYVPAPFCKDSIIEAAEAGVKLIVCITEGVPV
                     LDMLQVKVYIENNTQARLIGPNCPGIITPGECKIGIMPGYIHLPGKVGIVSRSGTLTY
                     EAVKQTTDKGFGQSTCIGIGGDPIPGTSFIDALSLFEKDPKTEAIIMVGEIGGSAEEE
                     AAEFIKSHVKKPVVSYIAGVTAPAGKRMGHAGAIISGGKGTAAEKYAALEAAGVKTVR
                     SPADLGDAIAEVTGW"
     gene            complement(29531..30694)
                     /gene="sucC"
                     /locus_tag="I9760_000303"
     CDS             complement(29531..30694)
                     /gene="sucC"
                     /locus_tag="I9760_000303"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAT5042098.1"
                     /translation="MNLHEYQAKQLFASYGLPVPRGEVAYNVEDALLVASQLSTSRWV
                     VKAQVHAGGRGKAGGVKLVSSKDELAAVAKSMLGTRLVTYQTDARGQPVNAILVEETC
                     EIDKELYLGAVVDRATRRVVIMASTEGGVEIEKVAHETPEKIFKVVVDPLVGVMPFQC
                     RETAFKLGLKDDQIKQFTHLMMGLGKMFVDCDLSLLEINPLVITKSGQLICLDGKINI
                     DGNALFRQPKLKNMRDVSQEDDRENRASDWELNYIPLDGTIGCMVNGAGLAMATMDVI
                     KLHGGEPANFLDVGGGATKERVSEALKIIVSDEKVKGILVNIFGGIVRCDLIADGILA
                     AVKEVDVKIPVVVRLEGNNAQLGAEILNKSNLNVIAATSLTDAAKKIVAAVSE"
     gene            complement(30757..31986)
                     /gene="odhB"
                     /locus_tag="I9760_000304"
     CDS             complement(30757..31986)
                     /gene="odhB"
                     /locus_tag="I9760_000304"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAT5042099.1"
                     /translation="MSIEVKVPVLPESVADATVAAWHKKVGDKVSRDENLVDLETDKV
                     VLEVPSPVDGVLSDILFNTGDTVGSGDLLAKISQSVSVESQKTEKEEKPVKKEEIKIS
                     ESESVSTKEDKSTSPVVRRMMAENDLKAGQIQGTGKDGRITKEDVLSYIESNREKSNQ
                     TAEVQKESSMRTLSFGGREEKRVPMTRLRAKIAERLLQAQHNAAMLTTFNEVNLKAVM
                     DMRSQYKDSFEKKHGVKLGFMSFFTKAVVESLKRFPAVNASIDGQDIVYHGFYDIGIA
                     VSTERGLVVPVIRDADQLSMAEIELAINDAASKARTGKLSMEEMQGGTFTITNGGVFG
                     SLLATPIINPPQTGILGMHKIEERPIVEKGQIVIRPMMYVALSYDHRLIDGKDSVQFL
                     VSVKELLEDPSRLLLNV"
     gene            complement(32022..34832)
                     /locus_tag="I9760_000305"
     CDS             complement(32022..34832)
                     /locus_tag="I9760_000305"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAT5042100.1"
                     /translation="MSSSDLQKEWASSYLSGGSMAYVDSLYEDYLADPDSVSEDWRAV
                     FSALPKVNGATKEVSHRDIRDYFLQNADKKLNKIIQIADSQQYQVASLINDFRSLGHL
                     AAKLDPLEMTERMPVPRLELAYHNLADVDVNRTFFAGTSFNGPEMTLGEIYNALRETY
                     CRSIGIEYMHISDTEVTEWLQHKMESVRGCPEFSKQEKLNILKDLIAADGLERYLGTR
                     YVGQKRFSLEGGDSLIPMMKELIRRSGVNQVKELVIGMAHRGRLNVLVNVLGKEPGLL
                     FQEFEGKIKYERTGDVKYHLGFSSDIKTESGAIVHLALAFNPSHLEIIGPVVEGSVRS
                     RLGRRNDLAKKDKVVPIVIHGDAAFAGQGVVMETFNFSQARGYCTGGTIHIVINNQIG
                     FTTSNPLDARSTLYCTDVAKMVQAPVIHVNGDDPEAVVFATQVAFDFRMKFKRDIVID
                     LVCYRRHGHNEADEPSVTQPKMYKKIKSMPTLREKYGEQLVSVNLLTKTELDKLVDSY
                     REALDKGKAVVDLVHEDYEGKQSLDWTPYINAKWTDKVDTTISKEELEKISSQLNKLP
                     DGMKLHPVVERLLAERNKMTAGEIPMNWGYAEIMAYASLLHEGYGVRISGQDSGRGTF
                     AHRHAALHDIEKGDTFIPLEHISTNPKRYFTVIDSVLSEEAVLAFEYGFASSEPSFLV
                     IWEAQFGDFANGAQVVIDQFISSGEQKWGRLCGLVMLLPHGYEGQGPEHSSARLERYM
                     QLCAQHNIQVCTPTTPAQIFHLLRRQVIRNFRKPLIVMTPKSLLRHKLAVSPLEALFK
                     GKFHTVIPEIDALDAQKVTKVVLCCGKVYYDLLQMRRDKNLNHVAIVRIEQLYPFPKK
                     ALMAELEKYQKANEVVWCQEEPQNQGVWFSSQHNIRDCLRSDQTLQYAGREFAAAPAV
                     GSPGLHAEQQVALVEQALLGKK"
     gene            complement(34890..35612)
                     /locus_tag="I9760_000306"
     CDS             complement(34890..35612)
                     /locus_tag="I9760_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAT5042101.1"
                     /translation="MADSRNVILSIYRYNPEVDAKPYMKDYEIEIPAKSDPMLLTLLE
                     RLKAEQDPSITYRRSCREGVCGSDGMNINGTNGLACITHLSQLKTDKIVIRPLPGFPV
                     IRDLAVDMTQFYQQYERIEPYLQNDEVAPARERLQSPEERAQLDGLYECILCACCTSS
                     CPSYWWNPDKFVGPAGLLQARRFLADSRDKATQHRLDLLQDPFSVFRCRTIMNCTNVC
                     PKGLNPTQAIAKIRTQMLTQET"
     gene            complement(35625..37394)
                     /gene="sdhA"
                     /locus_tag="I9760_000307"
     CDS             complement(35625..37394)
                     /gene="sdhA"
                     /locus_tag="I9760_000307"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAT5042102.1"
                     /translation="MTVARNRFDAVIIGAGGAGMRAALQMTNSGLKVALLSKVFPTRS
                     HTVSAQGGITAALGNADEDDWRWHMYDTVKGADYIGDQDCIEYLCKTGPEAVYELEHM
                     GLPFSRMDNGKIYQRQFGGQSKHFGGEQAARTCAAADRTGHALLHTLYQQNLKAKTHV
                     FSEWYALDLVKNSQGRIAGVTAMCMETGEIVFFQTRVCILATGGAGRIFQSTTNAFIN
                     TGDGFGMALRAGIPLQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLINKDGERFMERY
                     APRVKDLASRDVVARAMALEIRAGKGFDPKGVDHVKLKLDHLGADLIMSRLPGIRELS
                     MKFAGVDPIVEPIPVVPTCHYSMGGIPTNMHGQVITKTDGKEHVVEGLYAVGECACVS
                     VHGANRLGGNSLLDLVVFGRAAGIHVEELWQSNQLPDMSYVSEDDVVASLVRYERWEN
                     SKDGESHAVIRDEMQRVMQEDFGVFRTGEVMASGLKRLQALRDRLAHAKLDDKSQIFN
                     TERVEAMELDNLMATAYATALSAIARTESRGAHSREDYPKRDDANWIKHTLYFEEGEV
                     IDYRPVNTSPKYVEPFEPKERVY"
     gene            complement(37396..37743)
                     /gene="sdhD"
                     /locus_tag="I9760_000308"
     CDS             complement(37396..37743)
                     /gene="sdhD"
                     /locus_tag="I9760_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HAT5042103.1"
                     /translation="MVNNVTSLTGNGLKDWLIQRVTAVYFAAYLIFLFGFILANPDLN
                     FSQWHLLFANKLFQIATTIGLMALTLHAWIGIWTVTTDYMKCTVIRLSVQLLVVLWLL
                     GQFIWGLMIIWGQ"
     gene            complement(37737..38111)
                     /gene="sdhC"
                     /locus_tag="I9760_000309"
     CDS             complement(37737..38111)
                     /gene="sdhC"
                     /locus_tag="I9760_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HAT5042104.1"
                     /translation="MNKKRPINLDLSSLKFPPMAIASILHRVSGVLIFLLFPFVLFIL
                     GCSLQSEESFAQTKIILANPYYKLLLWAFGASMIYHVIAGIRHLIMDMGFGESLRAGR
                     MSAVSIIALAVILAIFLGIWIW"
     gene            38432..39571
                     /locus_tag="I9760_000310"
     CDS             38432..39571
                     /locus_tag="I9760_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HAT5042105.1"
                     /translation="MIAKRPIYNQQLKCVAFEILSCRNVILNEELTRQFYELIYHTDT
                     QLPLFVPFSLKKLLEQFETPINNPIILKLPADEIESVYSLTELQDSLFSIALLINTPQ
                     QLAWLNFAEYIGLTEQLMNKSDVTKVVQYSKAKNRKIIAYDLNQHVNFEKCRAMTMDY
                     YCGDFLLNYIAEEHTEIAANKLNVLQLIQELQKEDCDLNTITHIIQEDPLLSYQLLKL
                     VNSVGFSSGPSIESIDQAVARLGTINLKKWVMLFSMKNISDKPVEILESGLIRAHMAE
                     ELAKKSSNIFSQSAYTAGLLSILDCLLNKPMPALVEQITLAEEIKNALTNKSGPLGNL
                     LSLVIAYEEGHWEEVTQLNINGIDLSQLYIDSLALVSQGTKIMSN"
     gene            39721..>39896
                     /locus_tag="I9760_000311"
     CDS             39721..>39896
                     /locus_tag="I9760_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAT5042106.1"
                     /translation="MFFIKYLGALIYDLIILFILFFAYTSVLLLFTHGQAIPPATRWY
                     QFSLLSITLVYYYGS"
BASE COUNT        12637 a         8368 c         6833 g        12058 t
ORIGIN      
        1 atttctttat ctctctaaag cactcaaagt atctcaaagt gcccacacag tttgtcttct
       61 ctcttcttaa tgaacccgcc ctcgtggcgt agggcgtatt ctactcattt cagcatcact
      121 gtcaaatact tttttaattt tttttaaaaa attatctcag tctgcttgtt gaatactcag
      181 cttggcaatt cgacgttttc caacttgaat aatataagac ttgccaatag gtaattttag
      241 ggaaggatct gaaattttat ccccatccac tttaacagcc ccttgtttca ccattcttat
      301 agactctgat gtgctggctg tcaaatcaat ttgctttagt aattgtgcta aagctatcgg
      361 ctctacaata actaacgata gttcttccaa gtcttcagga atatttcctt tttgaaatcg
      421 ttcgataaaa ttgttatggg cgagttcggc ctgagtttga tcatgaaacc gtgcaacaat
      481 ttctttggca aaatcaatct taacatctct gggatttttc ccttctaata cagattgctt
      541 taattgttgt atttccttgc ctgttttaaa gcttaataaa tcaatatatc gccacattaa
      601 ctcatcagaa actgacataa tttttccgaa gatttgctca ggtgtttcat tgatcccaat
      661 gtaattatca agggatttcg acattttctt tacaccatcc aaaccctcta ttaatggtgt
      721 catcatgaca acttgaggtt caaagccata atgcttctgt aattctctac ccattaataa
      781 attaaatttc tgatcagtac cgccaagttc aacgtcagct tttaatgcca ccgaatcata
      841 accttgtaac agcgggtaca aaaattcgtg aattgcgata ggctgtcccg tagtataacg
      901 tttattgaaa tcatctcgtt ccagcattct tgccacagta tgtgttgcag ccagtcgaat
      961 caaatcaacc gcattaaatt tattaagcca ttgtgaattg aaagccacag tagtcttatc
     1021 agggtcaaga attttaaata cctggtgttg gtaggttttt gcattttcca gaaccgtttc
     1081 ctgactcaat ggcattcggg ttacattttt tccagtcgga tcaccaatca tggctgtgaa
     1141 atcaccaatc aaaaaaataa cctcatgacc aaattgctga aactgcctca acttattaag
     1201 cagtacagta tgtcccaaat gaagatctgg cgctgttgga tcaaaacctg cctttatttt
     1261 caatgggata cctttttgta atttcttctc caactcaggt acaggaagaa tttcctcaca
     1321 cccccgcatt aattcggaac aaactgaatc ttgaactatc atgtcattaa aacccgtata
     1381 aatgattgac ctatcttctc gcagcgagta tcttagcccg gttagcaatt tcctgctata
     1441 ccaaatgata cattttgaga taatttatta ctctaaattg agaaaaatat aacaaaatat
     1501 ccaaataaga gtcgatttag ttgactcaag gtagcggtca cttccaaata atgactgtga
     1561 tatattgatt gaaatttaaa tagcagcatt tttcagatga cggcaatata ggcaaaagat
     1621 ggataaaaga ccgaatatac caaaaagaaa atctggtaac tcatcaaagc tattagcgtt
     1681 ttttgcgttg gtatttgcct tttcgcttcc gtatttgcta gtgaaaaatt tttcacaccc
     1741 taaacgaagc agttatgctg accaaactga tttatacccc gaactatcag acgagttatc
     1801 aagagatata cctgctgaag cttccacgga aattccgcat gaaaaacctg atattaaatc
     1861 tgctacaaaa cctatgcaaa caaagcaaga tcagcaagat aaaccaatca atacaagtca
     1921 tgtaaaaaaa gataatgaat ggcaaatagt caatcctcgt tcaggagatt ctatggcgac
     1981 cattttccat aggctaggac taaccgcgca aaatttaaat gatgtgataa aaaataaccc
     2041 gcacgccaag atgcttacaa gaattaaacc caaccagaaa ttacaatttt taattaaaaa
     2101 aaataaattg gaaaaattaa ttgtccctgt aaacaccatt caaacactaa cagtttatcg
     2161 cgcagggaaa atatacaaaa ccaaaattga ctcaaaaaaa acaaccactc aaaatcgtta
     2221 tgtgactgca acagtccaag gttctttgta tacaacagcc caacgattta acatcccctc
     2281 aaaattagtc cgtcaaatga ccactatctt taacaaagaa attgattttt ccagatcttt
     2341 gcgctccgga gatcaattct cgataattta tgatgcttat tacattgaag ataaaatggt
     2401 tggagtagga gacattctgg tagtaacgta tactaatcgt ggaaaaactt atcaagcaat
     2461 acgtcatact aacgctcatg gtgagcaaga ctattttacc ccacaaggca ccagctttaa
     2521 aaaagccttc actcggtatc caattaaatt tagccatata agttcgactt ttgctttatc
     2581 gagataccac ccaattcttc attacagacg agcgcacaaa ggtatcgatt tggctgcccc
     2641 gattggaaca ccgatacatg ctaccggcga tggggttatt acaatcatcg ataggcataa
     2701 cggatatggc aatatgatta aaattaagca tgataaaaca tacagcactg tttatgggca
     2761 tttactcaag tttcagaaag gtctatcaaa aggcagtaga gtaaaacgag gtcaagtaat
     2821 tggttatgta ggacaaaccg gtctggcaac cggacctcat tgccattatg agcttcatgt
     2881 caataatcaa ccaagaaacc caactacaat aagcttgcct actgcagcac cagtaccagc
     2941 aagagaaatg gcggcattca aagcaaaagc cagtaccctt ttagcccggc taaaactttt
     3001 cgagcaagcg caattagcaa gtaaagggaa aaagaaaatt aacgttggat aaaagctgcg
     3061 cgtgggattt atccataccc aaatacctaa cttaaatgtt agcagacact tctttataaa
     3121 gcgagtaagc tccaagtaat tcaaacataa cgttactctt tttgtattaa aaaaatctta
     3181 tatcatgtaa ataaatatcg acaaattaaa accgaggcaa taattccagc taaatctgcc
     3241 aataatccag caggaatagc atgtcttgtc cttctgatac ccactgaacc aaaataaacg
     3301 gcaatcacat aaaaagtagt ttctgtactt cccatcattg ttgcggcggt ttttgcgatg
     3361 aatgaatctc ctccgtattt atgaatcagc tctgccataa ctcctgttga agcacttcca
     3421 gagaaaggtc tgataagagc taaaggtaat aattcggaag gcattcctat catagtcaac
     3481 gctggcgata agatctgact cattaaagaa aaaaaacctg aagctcttaa catgccaatg
     3541 gcaaccatca tcgcaactaa ataaggtata agctttacgc tcacatcaaa accttgtttt
     3601 gctccaatta taaaagcatc aaatacattt acttttttaa ttgcagcata caaagggata
     3661 ccaacgatga aagtcaaaaa caaccaattg gataaatgat tagcgaatcc attcataagc
     3721 tatctgtcct ttcaatttta aatcgcggta atttagccaa ttgttttaca gcaacgatag
     3781 ctaccagagt agaaacagta gtcgcgatca aagaagtgaa gaccacacta cttgggttag
     3841 tgctcccgtt tgcagcaaga aatgcgatag cggtgaccgg aattaattgc acactggaag
     3901 tgtttattgc caagaaagta cacatggaat tcgtggcaac tttcaaattc tgattcaggc
     3961 tctgcaactc tttcattgcc tgcaatccaa agggggtcgc ggcattagca agacccaaca
     4021 tgttcgcagc aatattcatt accattgctc ccatagcagg atgctcaaca ggtatatcag
     4081 gaaataatcg ctttaacact ggtttgagta ctttacctaa aatagaaact aaacctgatt
     4141 ctgtagcaac cgacattatc cctaaccaga aagtcattat tccagccaaa ccaagcgcta
     4201 actcaaatcc cagcttggca gagtcagtaa ctgctcttac tacctcgtca atacgcccct
     4261 caataactcc aaccaccact gaaatcagta tcattcctaa ccatattaga ttgagcatta
     4321 ttgcctccaa cctctgaaaa aggttaatat gtaaccaaaa ttagatttaa aataccaatc
     4381 aggacttatg aaacatactt acaatccctt cagatactta atatatttcg ctttttttat
     4441 caccagcctt tccagttata gcagccaaac aaccaacatt aaggaacaaa ctgatacagg
     4501 tttagcccca cccgcccctt cggttaccac taactcagct gctattgaag aacaggcgaa
     4561 ttcttcaatt aggaaactat atcatacact caataccatg ccgaacactt ctatggcaga
     4621 tagaattagc cagatcagcg catattttaa agggacaaaa tatattcttg gctctttggg
     4681 cgaaggtcca aatgctcgtt atgatcaatt tcccagatat cgagttgatg gctttgattg
     4741 tgatacctat gtcaatacag tactttctct agcattagct aactctcttg aatcattcca
     4801 ggagtgttta aaacatactc gctataaaaa tggaaaaaga tcttatatca acagaaatca
     4861 ttttacctct atagactgga ataactacaa tcagaagaga ggtttactta aagacattac
     4921 cttctctatc agaaatgaaa aaaagcagcc tgttgctctt tatgctaatg ccttaatcaa
     4981 caaaccacaa tggtataatc ataaaacgat cgataccatt cgattgcaaa aacaagataa
     5041 aaatgaacag gaaaaacgtc ttgtggaact aaaagccaaa ggtaaaaagt tggaaacttc
     5101 tctttctaat gtcccttata ttccattcac tgccttattt tcagagaata aacctaacct
     5161 gcatttattt tcccaaattc ctaacggagc tgttattgaa atcatcagac ctaattggga
     5221 cttacgccaa caaataggga cagaattaga tatttcgcat ttaggctttg ctatttggat
     5281 taataacgaa ctgttttttc gacaagcctc ttcccaatat ggtaaagtag ttgatgtatc
     5341 cttgattgat taccttgata aagcacgcag tagcccaact ataaaaggga ttaatattca
     5401 gattgtattg cctgaaaaac cagtatctaa tgggtgccag ctttttgata ttcgatagct
     5461 tataaattaa tatcttatgg ttaacctata attttcgtaa ttaaacccgt ataaccatta
     5521 attattttat gaaatggtcg atgtttttat taacgagata ctcgtgtttc ttaataaaaa
     5581 aaatcgattt ctattttaat tgactcgctt ggctattatc tagcagttgg cggctggaaa
     5641 tatgctcaat gatatgaaaa tagaaaaggc ttattttgat caaataagcc ttttaaagga
     5701 attatttctt tacttcagca gttgtttttt tagcttcttg caccaaactt aacatggctt
     5761 tttcaataat ttgaaatgat ttttgcatat aatccaaagc cttgtggcca ttttccactg
     5821 ccaaattaat ttgtttttcc agcatttctt cgggcttttt aacttgagcc agttcatcag
     5881 gcttcaaata acttaaccct tgcagggttt taacattcag ttcagcaatg gcttggaaag
     5941 gctcttgtat tttttttgcc atttcactcc atctttcaaa attgtattgg gtcataatat
     6001 tctccatttg ttgcactgca caatataata gtagacctta tctttattat ttgtcaatat
     6061 attatctaat aattttttgc agtgcaccaa aaatcacgat tacattcatt gatttttgat
     6121 gtgtattgcg ctttccaaat catcagtaaa attcgtaaag cgaaacattc tatttactac
     6181 aaaaaagttt gggaggaatt tttagacttt aaacattttt tgccattgat gtaccatttg
     6241 ttgcatttgc ttattccaga cttcagtcgc ttgctggtaa ggattatttt gtaaaggttt
     6301 gtccattgca tgaaacattt gctccatcat ttccttaaag gaaacatgca ttggatgatt
     6361 agctaatgaa atgatttgac gtaaaatttc tgaagacaaa aattgcgttg gtcccgcctc
     6421 catttccact ataatttgca acagaaccga attagttaaa tcctttcctg tctctgaatc
     6481 ttctatcttg aaatcaatac catccacaac atacttttgt agttgctcaa cagtaatata
     6541 ttgactaatt tcagtatcat acagtcttcg atttttgtat ttttttatga gtcgagtcat
     6601 tattttctca agtatcagat atagaaatat aattataaaa tgatgtgcaa aattggtata
     6661 gtttgcttct ttaagcccaa gagtaaatct ttcacacttc ttttacaaat tctttcaagg
     6721 ttaaaaaagt taatacatat gcaaaccacc attgacacta agatttgctc ccgtgatata
     6781 tcgacttttc tcactaatca gaaattcaat agcccaagca atttcttgtg gctctgccaa
     6841 tctttttgcg ggtatcaaat taataatccc ctccagaata tctggcctaa ttcctttcat
     6901 cagacgagta ttaacgtaac caggagaaat actgtttact gtaatatttt taatcattag
     6961 ttcctgggca agacttttgg tgaaaccata tactccagcc tttgaagcag cataattggc
     7021 ttgggaaaat tgccctttct gggcattaac cgaagacaca ttcactattc ggcctgactc
     7081 ctgagctttc atactctcta ctacttgttt agttacatta aacattccat caagattgac
     7141 acgcaacact tcatcccatt gccttttggt cattttggta aacacggcat ctcgagtaat
     7201 tccagcatta ttaatcagca catcaatacc gccaaactct tcaataatta agcctattac
     7261 ttctgcacat tgttcataat cagttacatc catatgcctg aaattaatat ttttatagcc
     7321 ttgtgtcttc aaatcatgca accatgcttc accctcatca taggaaacca aatccaaagc
     7381 aatgacatgt ttaaaagttg aatccagttg ttttgcaaca gccgtaccaa tgtcaccagt
     7441 tccaccagta attaatacca cattattatt cagcatgaat ttctctatta gttttttcct
     7501 gtccccaatt gaagatttaa aattggagct tatattttat tcattgttaa cggtgatcgc
     7561 agccactttt tcaaaacccc aaggtgtctt gcgaacagta aataatatgt tcacaaaacc
     7621 tcttgagatt cttaatcggt gaatcaaact ctgactacat atactgacca ccattgacgt
     7681 ccagatttgc ccccgtaata aatccgctat ctggtgatgc taaaaatgca acggcatctg
     7741 caatttcttt tgggtatccc aatcgaccaa caggaatact ggatatgatt gaattgagta
     7801 catcttcttt caatgcagcc agcataggag tttcaatata cccaggcgac actgtgttta
     7861 ccgtaatacc tttacttgct acttctaatg caagactctt ggtaaaacca aataaagccg
     7921 ccttggtgga agcataattg cattggccaa actgcccttt tcttccattg attgaagata
     7981 tactgacgat acggccataa cctctttcca acatcactgg aacgacattg cgcgtcatat
     8041 tgaatacact ggtcaagttg gcgtctaaaa cctgttgcca ttgttgaggt gtcatttttc
     8101 ttaaactgca gtctttagta atgcctgcgt tgttaaccaa aacatctata cggccataac
     8161 gctccatcac cagggatgtg attttttcgc aatcactata ttgagctata tctccatata
     8221 cgatatcaat atcataacca agttgctttt gctcatcttg ccattttttt gcttcttcat
     8281 gtcttccatc tttatagtag catgccacaa cttggtaact atcagctaag cgctgactaa
     8341 tagcagaacc aataccgcct gttcccccag ttacaatcgc tatcatttta tccatcgaca
     8401 actccttttg ttgctaggta acattacatt actacaatct aatttcactt tcttgtcaat
     8461 gacatatata taatactaag ttggtgataa taattcagct actttatttt tgatcatgtt
     8521 acggagctga tataaatgta ttaatgatga ctaatttgca ttacaaatcg aatgaagata
     8581 ccctaaatct gcttttaagt aagcatcgta atataagtgt tttttaatat tagaagtagc
     8641 attcagagaa tgagggtatt atgtttaata aatttttagt tacaacacta tccgtagtca
     8701 cattgacact ttcaccatat ggttttgcca aagatgattc atgcgaagtc caagtggctg
     8761 tttccactcc cccagttcaa tttgatcaaa gtgtggcgtt taacgtaacg aatgatgatg
     8821 ggattagtaa atccattatt ttacaaggcg gaagcagtcc aaaaactata gggaaattaa
     8881 tttgttctcc actaccttat accattagcg ccacccaata ttttacacca acaggcttaa
     8941 ccaataagca tgctcaacct gttggccaat gcgtattaaa agctggaaat gtcatattga
     9001 atggttctaa caatagcgtt gctgttgttt tccccaatga ttttatatgc caccactaaa
     9061 atattagctg cattgtgcat tgctcgggag tgcaaaacat cactccctgg tatataccgg
     9121 tacgtagcct ttcaattgtt gggcaaactg tttcagcaca tcaagacccg attcctctgc
     9181 ctggcggcac cattgctgca gagcttctac taattctttt tgagtcgttg ctgttttcag
     9241 ccaaacattt tgtaatgatt gcttgtaaga gtaaaccact ctcaactgtt cgcgggcatc
     9301 gagcagcttt tgcaaacgag ttttagcttt tgtcgataat agcttatcct ctcgacgcag
     9361 taatctacct gcccgttgaa acaatttctt gtcttctttt gtctcaatgc tggtgttttt
     9421 ttcatgcttc aaaataggtc tgatgacatt cttatagtaa taagacatca cttgaaagcg
     9481 atttgaaata actgctctca cagtatccag atcaacatgg aattttcctt catccatagc
     9541 caatttggga ggtaattttt ttaccttggc caaaccgaaa agggaaagaa tgcgaatata
     9601 catccaacca atatcaaact cccaccattt cactgaaaat ttggctgagg aagcaaaagt
     9661 gtgatgatta ttatgtaact cttctccacc aatccaaaat ccccagggaa tgatattagt
     9721 agcagcatcg ggacattcaa aattcctgta tccccaataa tggccgatac cattgatgac
     9781 tcccgcagca aacagcggaa tccacatcat ttggattgcc catacagtca ttccaggaat
     9841 cccgaagaaa aataaatcca gtaataacat cagcaaaata cctttacttg aatggcgcga
     9901 gtagacatta cgctctatcc aatcggttgg tgtgccatga gaatatttgg caaccatatc
     9961 cctgtccttg cgtgcctctc tgtaaagctc agcgccttgc caaaatacct tcctgattcc
    10021 ctcaaccact gggctatgag ggtctccctc aacatcagtt gttgcatggt gcttgcgatg
    10081 gatagcaacc caatccgcag taaccatacc cgtagttaac cataaccaaa aacggaaaaa
    10141 atgactgacc acagggtgca atgttagcgc tctatgagtc tggttccgat gaagatagat
    10201 agtaacagat gcaattgtga tttgagtcag gataaaagtt gcgagaacat atccccagaa
    10261 agacaggttt aaaaagccga ataccataat aaccccacat tgtacggaat tataaaaata
    10321 actataattt aagagtgaat gtattttatt cctaaattat agaatcaata tatgtgtaat
    10381 gataacacat tttttatcat ttcattgcat gatttcatga tgtagtcgat aataaattta
    10441 ctcatcttat tttatcagga tatagacatg agcgataaag ttcagaaatt gattgaaaat
    10501 attgtgcctt ttatagtgat tggtgttggg atcgcaatag ttgtagcctt attgttcatg
    10561 tttttttatg tggccatttg gggcgtaatc atcggtggaa tactctggct tggggcctta
    10621 gctaaagaat atttcttccc cagcaaatca tcccagaaag aacaaggaag aattatagaa
    10681 catgatgata aaaaataatg cctgaattac ccgaagtcga aacaacaaag caaggtatta
    10741 aaccccatct tgaaggccgt ataattacca cggcgcaagt gcgtaatcga aaacttagat
    10801 taccagtacc tctaaacctg aatgaattat gcgaaggaaa gcatataaca gctattaccc
    10861 gtagaggaaa atacatctta cttcatatgg ataaaggtta tattctcatc catcttggaa
    10921 tgtccggaca tttgcgtatc gtctcccaga cagcaaatcc tcaaaaacat gatcatgttg
    10981 atctgcacat taataatggg cttgctctca gattctgcga tccaagaaga tttggcttat
    11041 ttatttacat tgatgaaaat ccttaccaac acccactgct ggctcattta ggcccggaac
    11101 ctctttctga tgactttaat agcgagtact tattacgtaa agctgcgaat aaatcgcaat
    11161 ccatcaagtc atttattatg gatagtcaaa ttgttgtcgg gataggtaat atttatgcgg
    11221 cagagagtct ttttcttgcc aaaatccatc caaatacctc tgcaaaaaag ataactacag
    11281 aagaattcaa ttctctgact ggtcacatca aaaagatact tgaatctgcc atagaggcag
    11341 gagggacgac acttcgtgat ttctattctt ctgatggtaa accagggtac tttcgttttg
    11401 ctttaaaggt ttatggcagg aaaaatctcc cctgtttggt atgtgaaaat aaaatagaaa
    11461 ctgtcgtgat tgccggtcgc cactccgcat tttgcccgca ttgccagcca atcattacat
    11521 aataagttta ataatggcta cctggaacca tttttgtttc ttaattgatt ctgttgttgt
    11581 gacaaaaggt taatttatgg atattaaaaa acttaaaaag gcagcctctg attttctgga
    11641 aagatatcca gacgggttct gccaccctga aatgattgaa atgggtaaaa aacataaaat
    11701 ggataagatg gttgctttgg ctcaaggaag ttttgccagg caaaatttta aaaaccctga
    11761 tgaaataaca aattccatgt tgaaaattgt tactcagtca tctctgattt caatatttga
    11821 aaaacctaaa tttagagatt ttattaaaag aactcctcca aacgataaag tacttattgc
    11881 aaaaggatta aaagaacttt tgtatggaaa tgaacaggaa ggctttgaag agcttctttt
    11941 tcaatttcaa agagagaaac tggcgaagtg gactttaatg actgtttttc aggcttactt
    12001 caatccggaa gtggatgtgt ttataaaacc tacaacaacc aaaaatatca ttaaagaact
    12061 gaaccttgat atcgtatata acccaacccc ttcctgggag ttctatgaat cttataggaa
    12121 tatcattaac cagatgaaaa caaaaattga aaaatcacta gcgcccagca atgcggcatt
    12181 ctgtggtttt ttaatgatga gcctggatga tcgttaaatt ttctatgagt atcgcttgat
    12241 gggctctatg aagacatgcc acattctttt attgagcggg tgatgggaat cgaacccacg
    12301 ccatcagctt gggaagctga ggttctgcca ttgaactaca cccgcaaaaa ctcacgaacc
    12361 ctttcgtcaa tagaacatta aggtatcatt ttataattat tgattcaatt aagttatcac
    12421 aacggcgatt taaatttaaa aacaggtttt aaaactcaac tattaactta aactatgcaa
    12481 atgtatggca agcataactc acagaacata gcttggttgc ttcttttgat tgcaatctta
    12541 tattgatatc taaataaagt aaaaaatagt tattctgtag actaaacttt acagcttcaa
    12601 gaaaatcagc taagacctaa tatgaaatct ctgttattgc aatctgtgat tgaaaaatga
    12661 atccaataag aaaaattatc catattgata tggactgttt ttatgcggca attgaaatgc
    12721 gtgatttccc tgaactggca aataaaccta tcgctgtagg cggggatgca aaacgtcgtg
    12781 gagttatagc gacctgtaat tatgctgccc gccaatttgg catacgctct gcaatgccaa
    12841 cagcccatgc gctcaaactc tgccgagagt taatcttgcg gccagtacgt atggatgtat
    12901 accaaaagga aagccaatat attagatccc tattaacaga atatacggac ctcgttgaac
    12961 ctttatcatt agatgaagcc tatctggatg tcactgaatc cacacaatgc cagggaagcg
    13021 ccacctggat tgctgaagaa atacgcgccc gaatttatca aacaagacaa ctcactgcaa
    13081 gtgcgggcat tgcgccaaat aagagtctcg caaaaatagc cagtgattgg cataaaccga
    13141 atgggcaaat ggttattaga cccgaggacg ttagcgcatt tgttcttgat ttaccagtac
    13201 gcaagttatt tggtgtaggg cccaagatgg aggaaaaatt gggtgcgctc aacattaaaa
    13261 cctgtgctga tttgcagcga tattccgttg aatatttatt acaaaaattt ggcaccatgg
    13321 gtcaaagact ttatgaatta gcgcgaggca ttgataatcg ccccgtcaat cctgagcgta
    13381 ttcgaaaatc catcagcgtt gaagaaacct atccaaaaga cttgccaaat agtgaagctt
    13441 gcctggcagt cttgccagat cttatggcgc gccttgaagc aagaatacaa cgtgcaggta
    13501 aaatttctgg cattcacaat ttatttgtaa aattgaagtt taatgacttt cagcaaacaa
    13561 ctattgaacg agttatggac aaacttgatt taatcgtttt acgtcaattg atacaagaag
    13621 gttttgccag aagaggcatg ccagtacgat tattaggtat aggaattaaa ttgaaacaag
    13681 aaaatacgta tcaaagcata caattgccat tacttgactt ataacaagta tctaaacagt
    13741 tgatatcaga ttctttccca aacttgccat aattatcaga acttaataca tcagctctct
    13801 tttttatgca acaccctgca atagttagca aaatcaggac ttttatattg aaaatatcaa
    13861 tgcaatggaa gtgcaaaact cctgccatat tattaatgag ctattcttga ctgagctata
    13921 atctatacca tatcgctcaa tttgactcag ggaatactat ttccttgtag gcagtgatat
    13981 gctataaaat aatctccgga cgagacgcgc cgtacccggt taagacaaga cgacgccttt
    14041 aatcaatcta aaaggagtcg ttatgctact accctggctt tatttaatta tcgcagggct
    14101 cttggaaatc tgttgggctg tcggtttaaa atataccgat ggtttttcga aatggcagcc
    14161 aagcctgttt acagtcacca cactgattat cagtatgttt ttattagcca aggccgccca
    14221 aacattacct atcggcactg cctatgcggt atgggtaggg ataggttcac tggggactgc
    14281 aatcatggga atactgctgt ttcaggaaca tctatctgtc ttccgattgc tataccttgt
    14341 attaatgctg atatctatct ttggtttaaa atacacggct tgatttgaat cgttccagta
    14401 ttcctcaact tgttgtatga tggccctctc ataatcccat gcgctctaac gatgaaaata
    14461 agtcgattaa aaactttttg gataattacc cgcctatttt attacacagg tgttgcttgc
    14521 actcgctcca tatttaagta tctttttagc acccctacga ggccatgggt tgataaagtc
    14581 atccaccatt gggttgatca actcttaaat gctgtgaaag tagaatataa agtaatcaat
    14641 ccacataatg tccagcctca gcctggcaag gcaacgatcc ttatgtgcaa tcattccagt
    14701 gcttatgata ttcctttagg cttcaaagca ttccctaata actctattcg tatgcttgcc
    14761 aaacgagaac tctccaaaat tccactttta gggaagggaa tggctgcagc ggaattccct
    14821 tttattgaca gaaaaaacag atttcaagcc ataaaggatt tggaatttgc ccagcaactg
    14881 atggaaagtg gaattattct atggattgca ccagaaggaa ctcgctcaaa agatggcaaa
    14941 ctagccccat ttaaaaaagg tggttttatc actgctattc aagccaaggc tactataatt
    15001 cccataggaa ttcgcggagc ttttgatatt ctccctgccc gcacctttaa tatacatttg
    15061 aatcaaaaag cagaaattca tattggcgag cctattgatg cagctcaata tacaattgaa
    15121 aataaagagg aattaatcga aaaaacccat caatcaataa aaaagctggt tggggagaat
    15181 tgaaaaaact cacattccgg tttgctgaaa aagatgacta aaaataccta caataactag
    15241 aaaaaaaacc acgctataca aaaaataaaa ttgatttcta caatagaata aacaatccag
    15301 gctatctgtc ttttaatatc ggtactaact gttttgaaat ttcctaaaaa ttcacctaga
    15361 ctttaaaaaa gaaaatcaat atggccgtgc catgagcatt aataaaatag aagaagacag
    15421 ggatacctct tctgatctgg aaatgaaaac caagatgtac ttagtacacc gaatggataa
    15481 agccgggcca ttaacagtta aagatgttca catcctgata aacaagttaa gtaaagattt
    15541 gcagaaaaat caattaacat taattgattg tcaactaata ataaatcttg ttgaatcccg
    15601 ctttactttg gtcaaacaag caacaagaca tctacagttt tttctaaata aacttgatgg
    15661 caaatcaatt aacccaatct taatcccact ttcaaataaa cccttttggc aaaatgaacc
    15721 acatccattc gatcattatc gatctaccga tcaactacct gaaaaagcag atatcgtaat
    15781 cataggtgca ggtctaactg gcgcttctac cgcatatcat ctgatcgatg cagtcaagaa
    15841 aagaaacctt actgttgtat tgttagataa aggaaatcca gccactgaag ccagtggtcg
    15901 caatgggggt aattttgaat tattacctga aaactctgtg ggaatctata gtggtttagt
    15961 ctcagagcgg tttcgttttt taaaacgatg ctacccccat gttcatccgt caatattaca
    16021 aattgaaagc gaataccatg cctcactggt ttttcaattt gctttaaaaa atcgcaatcg
    16081 ctttaaggaa attgccagaa aagaacgtat ttattgtgat ctggcagcaa aaggatggct
    16141 ctacattgct catactgaag aggaagaaca agcaatttgt gatgaagtca ctctggctgc
    16201 ccaacatggc gagcgcatct acatttggtc tcgaaaaaaa attagagaac aattcggcat
    16261 caactccaag ttcataggtc gatttattcc agatgacggc acctacaatc cctttaaata
    16321 tacgtgctgt ctgattaagg cggcaatcaa aagtggtatc aagctctata cgtttgtgaa
    16381 agttcaacaa attaagagca tggatacaga ataccatcaa ttgaaaacca atatgggttt
    16441 gcttaaggct cggcaagtaa ttgttgcaac aaacgcattt actagtgagt tgctcccgga
    16501 attgagcgcg attaagcctc accaaagtca aattgcaatt accgagtcaa caccagatta
    16561 ctgtaagggt cgattaataa ccagcgaaga tggaccactc tatctcaatc aacccagagt
    16621 cagatcaaaa aatgggttgg tgccattact tattggtgcc ggtgacgacc gaccaatgaa
    16681 aaacccttat tatcgcaagc gttcaaaaaa agttcatgat cttattgtgc agcaaagaga
    16741 taagtatttc ccggatctta aagggaaacc attttcaacc gagtgggttg gcgccttagc
    16801 ttttacacct gatcaattac ccgctattgg ttacctgtct ccaggaataa ttattgtgat
    16861 gtgctgtaat ggatatggag gaagctatac cacagcagca ggatttgccg cagctgaaat
    16921 tgcattaact ggaaaaaatc ccccatggtt acccgcagat gtattttctc ctaaacgcct
    16981 cctgtctgac aagccgctat ttttggaaga tagcgacagt ttatggcgga ttggaaaaac
    17041 actttgtaca acacttaata atctcaatca acttctggct gagtcccttt catatcagtc
    17101 catgtatcaa ccttattcta caatttcttc attaccgcat gggcaccaag agaagacttg
    17161 caatcttgac ccactaagag taatgcatca attagcaatc tttagaactt ttgcatatca
    17221 ggaattagaa atgttaattc gctatacctc gcctgtttgc ttaaataagg gagaaattct
    17281 ttttaaacaa ggtgatgcgg gacattcttg ttttattctg attcaaggaa aaatcaatct
    17341 ttattatgag catgctgaag gctttacttc aatggttgca gaactggagg ccgggtcagt
    17401 atttggtcaa atggcattat ttcttgagaa taaacgtatg gccacttgcc aagctacaga
    17461 gaactgttcc atgttagaaa tcatggtaga accctgtaaa tcgcttttta ttcagcaaca
    17521 ggctcttgga attaaattac tccgcctctt aaaccaggga gtcatcgatg cgttacacaa
    17581 actcaataag cgattgaaat acacttaaca aaaatcataa aacaaatatt gattttgatt
    17641 gctttattca gcactaacaa tttacgcacc aagtgtagct ggccgattca ttttaaaaat
    17701 gtattctagt aattaacccc atacccatat gccatattag atatcgcagc agctaaacca
    17761 atatgtcttg gatcagaagc agccgttaag acattacttt ctttgtccca tgtaatggct
    17821 tgcatatctc cataatattg tttcaatggc attaaatggt atcccatagc ttgtaaaccc
    17881 tgttgaattg aacttgggaa agtatcaggc tcaaattgca aaacatctgg taaatattga
    17941 tgatgaaatc gcatggctga aaccatactg atagctccat acccatcata aaataatagt
    18001 gaggcaagta aaaccatagt aggaatacgg cttcctccag gagtccccag gatagctaca
    18061 cgccctggca tctccaggaa ggttggagcc atgctggata gcggcctttt tcttggttca
    18121 atggcattgc gctcacttcc cactaaacca aagacatttt gctcacccac tttagtagaa
    18181 aaatcgtcca tttcatcatt taataatacc cctgttccct tcgcaactac gctggagcca
    18241 aaaatgtaat taattgtcat tgttgcagca acgcgattac cttccgtatc aataatcgaa
    18301 atatgtgagg tatttgagtg ttttcctatc ggaactgagc cttgcaagac tttactatca
    18361 atcgctttat ctggtgaaat caagcggctt aattgtttcg cattatctga ggaaatcaat
    18421 ttatccagag ggatcttaat aaaatcagga tcacccagaa attgatctct ttgccaataa
    18481 gccaatctca tcgattcaac gacgtaatga acccatttta atttgggaaa tgaagacaaa
    18541 ggaaatttgg ataatatatt tagcatggtt accaatgcca ctccaccggc tgaaggcaga
    18601 ggagctgtga taattaacat attatgataa gcactaatta aaggtggcct cactttaatt
    18661 ttatattgag ccagatcatc aagagaccaa attccacctg ccgcattgac accttgaact
    18721 agcagagaag ccgtttctcc tgaataaaat ccggcatttc cgtacttggc tatcaatctg
    18781 agtgtttttg ctaaatcttt ttgaacaagt ttttctccaa tttcaaaagg cttatcattc
    18841 tttaaaaaaa tagcggcggt atcgggatat ttcttcaagt tttccaatct atcatccaga
    18901 gtggagaaat aacgaaattg aggatctaca ggaaaaccct cttccgcaag cttaatagcc
    18961 ggagctaatg tcttgctaag cggaagacgt ccatagttct tggctatata aaccaaggca
    19021 gctggctgcc ctggtatggc cgcagataaa ccaccattta gagaaaggcc gggaatcact
    19081 tggccgtcag gagccaaata catatcctta cttgcggcca gaggagctac ctccctgcca
    19141 tcaataaaga ggtttaaatg ttccttttcc tgatgaagta gccaaaaacc tcctcctcca
    19201 agccccgaat gatatggctc gactactgcc aaggcagcag ctaccgcaac tgcagcatca
    19261 aaagcatttc ctcctgctgc caaaatctcc aaaccggcat gagttgccaa ggggtgagca
    19321 cttgctacag catatccagg agggtgttgt ggaaatgttt ttgcattaac ctgataaata
    19381 ttacaaatta aaaaaaataa acaactcagc catttatagt tatatttcat tttgtctctt
    19441 ttttaattct ctaactacat tgggcggaac aaattgagaa atatcaccat ttaaggctgc
    19501 aatttcacga actaaagtgg atgagatata cattaaatgc tctgctggag ttaaaaataa
    19561 tgtttccacc tttttagata gctttctatt cattccagcc agttgaaatt catattcaaa
    19621 atcagaaaca gctcgcaatc cgcgtaaaat aatgcctgcg ctttgttcta atacaaaatc
    19681 aattaacaaa ttatcaaaac ccacgactct cacaccagtt aaatgtccaa cggactcttc
    19741 cagtaaacta attctggttt cccaagataa ataaggtcgt ttgttcttat tactcgctac
    19801 tgcaactatc aattcaggaa aaatagtgct ggcccgagta ataatatcaa tatggccatt
    19861 ggttacaggg tcaaatgttc ccgggtagat tgctttttgt ttcatctcat taattaccat
    19921 ttgcatatag aagcagtcta gcgtattgta aattgaaaaa ctataaaaaa atggaattga
    19981 ggtaagcaat gaatgcgata aaacgtttga caatcatttt aatttgcttt ctaacagcat
    20041 gcgctccccc aagaccctct gaagagccgc ctgcaaaccg tgaaattcct cttgaagaac
    20101 gcaaagccga aacagccact gtttcctcat gggaaattaa aggcgccctg gctgccaaaa
    20161 ccaaagcgaa aggctggtct gctactatga attgggtaca acaaggggcc aactcatacc
    20221 aaattcgttt aatggggcca ttaggtggtg gaaccgttct aatcagcaaa aaaggcaata
    20281 ctattacatt tcaagatggt tccaaaataa ctacttcgac caatgccgat gagttattac
    20341 taaaacaaac tggcattcga cttccagtaa ataacttgta ttactgggta agaggtttgc
    20401 ctgccccagg tggagtccaa tctgaaaaac atgaccaatt caatcacctg atacaattaa
    20461 agcaaaatgg ctataccatt gattttacta aatatacttc agtcaaggga atagatttac
    20521 caagcatgat tcgccttgaa ggaaatggtg tcatggtaaa ggtagtgata aagaattggg
    20581 ttgtataatt taattcttgt aatttatttg ctttctgaag ttgacatcga taaaaaagca
    20641 ctgcaaaata cgcagcacat tgcagggatg aatactattt ataaactcat ggatgattaa
    20701 agaatctaca attatagggg tgtcgccaag cggtaaggca cagggttttg atccctgcat
    20761 acctaggttc gaatcctagc acccctgcca ttttcttgtt gattcaacct agaatatgaa
    20821 gttgaatcta ggtacaagta aacagacggc agaagataac ctagaaggat gcgtacttcg
    20881 tatcgctcaa ataataataa tatctttttg gctgtcttct gctatgtgtt taagttatgt
    20941 gattaaccta aatctaaggc ttttgacaca tgtccacgat gatgattttt acagggaatg
    21001 ctaaccctga attagcacta aaaatttcct cacatttgca aatacctatt ggcaaagcca
    21061 ccgttggtac cttcagcgat ggagagacta tggttgagat tttggaaaat gtccgaggta
    21121 aagatgtttt tgtcctccaa tcaacttgtg cgcctgcaaa taataattta atggaattgc
    21181 tgattatggc ggatgccctg agaagatcat ctgctggtcg tattacagct gtagttcctt
    21241 attttggcta cgcacgccaa gataggcgag tgcgctccgc ccgtgttcct atcacagcta
    21301 aagtggtagc agacatgatg gcttccgttg gaatttgtcg agtccttact gttgatttgc
    21361 acgcggatca aatacagggt ttcttttata tgccggtcga taacgtatac tcaactccgg
    21421 tattgctgga agatataaca aaacaaaaat taaacaacat catgatcgta tcgccagatg
    21481 tcggtggggt tgttcgtgcc agagcagttg ctaaaagact gaatgacgct gagctgtcga
    21541 ttattgataa acgccgtagt gggccaaata aatcagaagt aatgcacatt attggtgagc
    21601 ccgccaataa aaactgtatt attgtagacg acatagtaga tactgctgga acactttgta
    21661 cggctgctca ggaattaaag aaaaacggag caaaaagcgt aagagcttac attacacatc
    21721 ctgtgttgtc tggtcccgca gttaacaata ttaaacattc aggtcttgat gaggtggtag
    21781 taactgatac cattccttta tcagccgaag cacaaaactg tgaaaaaatc agagtggtca
    21841 gtcttgctga tatgttagct caagcaatca aacgagtaaa tgttgaggag tcagtaagct
    21901 ctatgtttgc tgaataaaat gaaatattca ttgataataa aaagggagcc aaggctccct
    21961 ttttattatc aatgtttctt aatcccttgt accaacgtat ttgtcatcaa aataacgtct
    22021 taatttagtt cttaaagttc cacgactaat tcccagcatg attgcagcgc gggactgatt
    22081 atacttgcaa tgttccatta ctgcacgaaa tagaggggtc tcaatttctt caagtacaaa
    22141 ctggtataag tcaacaggat cagttccctt aagctctgaa aaatattttt gtacggattg
    22201 agtcacgctt tcggctagtg aagcagtggt atctcctgca tcattactga ttgttgtcat
    22261 tattattagc tcctcctttt aaaagcggta tattaccgaa ttgagcaata cgagacaagg
    22321 gcagaacaga aaaaactgca ttaaaattca atatatactg tttgcacttg aatcattgct
    22381 acgtttctca ccatagtgga ttgaaaagag gataagccgc tctcaacagc ttatcctata
    22441 tttaacgaac tactggaatt tcatcataga cggatcgaat ggtttcccat taactgtgaa
    22501 ttttccttca ctcaaactca tctcaatgac atagtctttg ccatcgacag taattaaacc
    22561 tgattgctcc aactcattaa tttgcttatc tacttgcatt gcagcgattt gctcaggagt
    22621 aggttctgct tggtttgatt gaccttgact gctttgcagc tgttgaatta acgcttgttg
    22681 catttctggc tgttttgcca tttgttgcat aatcgattgc tgcatcagtt gcttcactac
    22741 ctcgattggc atttttaact tggcattacc ttgagttttt tgaatcaatt caaagggatt
    22801 agaattatcg cctttgggta aagtaattaa caagttacca tcaatcatgc cttgtggaag
    22861 tttgagactt aatttggaaa tttcaaactc cgcaccttta ctaaatagct ttggcaattc
    22921 tggcaacatt gccattaatg cttgttggcg ctcttgatct gtgcctcttt gcatcgcatt
    22981 agcttgttca ttgattttag ccagtacctc agcatcaagg tttcttaagg acatctcgac
    23041 ttcaccagga ccgtaatttt gcccattggc gaataacgat tttaaagcag cactaaaatg
    23101 cgtgctgaag agattgtcat ctatatcgct gcttgagctt aatgtcaatt cactaatttc
    23161 aaacatcttt tgatctttaa caactacagc aagtgatggt aatgaaaaac tggcttgacc
    23221 caaatataac cctgtagtcg ttttatgtaa gtcatactcg ctgattacct tacccaaaga
    23281 gagcttggta tcatctttag aaaactctgc accatcaaat tcaatttccc cttccacttt
    23341 atccaaattg gaagacattg atgtagtgct gtccataccc atccaattaa atttaccatt
    23401 tccatcttta gcagttaatt tgaaagaagg aatggacaac tccaccgtgc ttttattcaa
    23461 ataatttaca aaaatgctca aatccagctg aggcttttct gaatcttgag aaaaaagctc
    23521 attgaattgc tgattatatt tttctggaaa tggaaatacg gtttgtgcaa agcccattcc
    23581 aaaacgtaac tgcttattcg cgtatataat ggggccatga taaatcttaa tcggcatttc
    23641 catttgataa tcttgggcag gagtagtttg caattttcca tcgtcgtcct ttactactcg
    23701 ctcaggtacg tgtaaacgcc atttaactaa tgcatcagag caaaaccaac ctctcttata
    23761 ttgctgaatg tcagcgaaaa gcccattgga ttgattaatt acctcgacag tttttttaac
    23821 cgttctttcg gttagaattc ccatgccata atatccacct aggattaaaa cagccaggat
    23881 aattactaat cctgtaagtt ttttcattat tatctccatg ttattaattt gcaaatagca
    23941 ttactcgagc gcagcaaagt gctgcgaaaa gtataacagc aaataaccta aaagtgcgac
    24001 agtctaatgc aagaagatta aaacttaatg aaaaatattt tcattaagtt tcataaattt
    24061 gatgtgttta cagcaaaaat caaacaatga tggcatattt atcatgcaat gctcaatgtt
    24121 tgatttttgc tatcttaatg tcttatgggt ttgcaataag tctctttaac agtaaacgcg
    24181 aggattaagg ccactaagga acataaggga agtaatttta gaccggcttg gaaatccgat
    24241 aataataacg tttcaacatt ataggctctg ggcccagaca ctatgtctat gatacgccct
    24301 actaaaggct ggaataaggc cccgacaaaa attgaagtca tattggtaaa tgcgatagat
    24361 gtaccaacaa cactcctgtc atgtatttct gtagaaacag cgtatgaaat cgcaactcca
    24421 gtgtttgtaa gaccaaaaac aaaaaatagt atgtaagccg ttgtttgact catttcagga
    24481 ataaaaacaa ataaactgct caagatcaca ccacatactg cggaaataat catcaatggc
    24541 ttacgacgtc ccattttatc agaaatccaa cctgataatg ggccactaat tccccagcct
    24601 ataaagatca atcctgttgc aaatgcagct gcgtgcgccc ctaaaccacg gccgaactgt
    24661 aaatatgccg gacctattgc ctctccaatt acagcggtgg ggccaaataa aaatccagca
    24721 tacagcgcat taagccaagt ttgtttattt gataaaatga tctttaagct atccaagata
    24781 ctgattcgat taacagaacg aatttcattg cgatgctcgc cgggtttatc ttgcacaaat
    24841 tgatacaata atcctgataa ggcaataaat aagaacgcga taatcagcat actatggcgc
    24901 cagccaacat tactcactaa aaatgaaaca ggggcctccc ctgcagcagc accaagcatt
    24961 cctaacgctt gagttaatcc agataataat ccgagcatag ttggaggaaa ccatgaagtg
    25021 gcaaggcgaa gtgagcaaat gaatgcaaaa gcggcactga aaccaatcaa cattcttcca
    25081 attgatgcag tcagcagacc atccgcaagg ccaaatacac agcaaccaaa agcagtaact
    25141 aatgacataa tagtcaataa ccatcgtatg cttaatcgat caaccgtcaa tcctacagga
    25201 atttgcatta agatatatgg aacatagaag gatgcagtta atatgccgaa gccagcctca
    25261 ttaatgccaa agtccatttg tagatatcgg tgcattacac cgggcgctac tctagctaaa
    25321 taatcagaaa aataaaaagc agctgccaac ccccaaacta accacgcaaa acccaggtaa
    25381 tttttacgtt tataaatttc cactttagat attcctattt tggtataacc accacatgct
    25441 gtatttattt aaaaaatgat caataaatta acaatgataa cttatcatta tactgctgtt
    25501 gtaacttatt tcaacatata gtttattttt acctttgccc tattggtaat gtattgagga
    25561 tttaggatat aatcctaatt tttaatcact catcgtcatc cagtaaaaca aaaatcctct
    25621 ggattgagtt gaatgattaa aagactagga cttacgaaaa aatccaaggt cgaggaaaaa
    25681 atgttttaaa tgagagaatt tagacaaatt aaatgactga attaaaaata ttttttaacc
    25741 aagagattgg aattttcgaa tatccagtgg atgaattcaa aacaaaactg aataatcccc
    25801 gagggttcac caagaagttt taaagagagt aaggcaaaat catattttga ctatataatg
    25861 aaacattcga gtcgcttcac ctgtgcggat gaccaaaata ggttcaaatt tatcaattac
    25921 cagggctcat aattgactga aattggtatg cccgtgtccg gttaaagttg aatcttcata
    25981 tattttcatg tatggaagtt ggttactctg aacaagttta aaaagcaaat tttcaagata
    26041 ctatacagac atgggatcca cccctgatga acaaactaaa tatagccatt aaagaagctt
    26101 ttgaatcttc cggaagctca aaagaagcga ataaagttta ccttgaattt atcaaggcaa
    26161 attttattat tcctattgaa tccaattctg atgatagtga acccagagta ttatttttgg
    26221 aagaaaataa ccatgttttt ttgccggtat tcactgatat gaaatacctc gatgcttggg
    26281 caagtgaaat tagtcaacat atcaaattac ttaaattatc aggggttgac ctgctgaaag
    26341 gtttaggtga gaaagttaca gtttgccttg atattggcag tgagctttat aaagaattca
    26401 atccatctga aattgaacga atgaggtcga tggttttaaa actatttaaa aactgaactc
    26461 aatacaagga acactcctat tcaattgaag aagcattaca ttatgctctt caattgaacg
    26521 tgatgatcat gctacgagct caagtaattg gtagcctact attgcggaag cggctatcgc
    26581 accaccaatt tcaaagacta aagatagaaa gctgttttta tgatgatatt gctgatcatt
    26641 tgcagctgtg gttttatcta aaagaatatc ttcctcgata ttgttcaaat caaataatgg
    26701 tttttctcca tatatgccag cccaccatcc ttcgagccat tgttctgctt cttgagaacc
    26761 ttcgcggaaa ggattatcct cttctggaac ttctgccaat gcacattcat acccaaaaat
    26821 ataacattct tcatagcttg ggtgttcaat gttaaatcgt aatctaagat gtgataataa
    26881 tgatgttgta tcgttcatga tgatagcctc ttttatgtat cctgaatata tttccgcttt
    26941 tcaactcatt tactcctaaa tctataatta ttaacgcaat tggtgcagaa ctgcttttta
    27001 gtgcatgtaa ctcccacact taaatcaatt ttacgacttt aatttgcccc ctgccttcga
    27061 aaatcaaata tttacattat cttgacaaaa atctttttac ctttggttct ttaacacgat
    27121 acttcattaa cttattgatt ttattaatag agatgcaagt attaggccaa cttttttatc
    27181 ttattttttc atggattttt atgattaata aatagtttta ttgcttaaaa taacaactgc
    27241 caagaacgat aaattatccc gctctaaccc atctatcccc aacagaatat tgataaaacg
    27301 gacacatcat tctaaggtca aagctcaaca tatttttaac aaagagattg gaatttttcg
    27361 cgagtcctga tttataaatc tgggaaaatt tgcgaatccc gtgtgcttat tcaaaagaat
    27421 tcaaccatta actaacctgc catgaaaact catccttcta tatctcaata gtaaaatgat
    27481 gccggcagga atgatgacaa aaactgccgg tcaaggtgcc aatctacggt gcgtccattc
    27541 atgaccatgc cggtaataat gaattcgatc atgtaatctg ttatctctac cctgccaaaa
    27601 ctcaatctca tccgggatta tcatatatcc accccagttt tccggtctga ctacgggttt
    27661 ttggccatat tcttcaatca actgatttaa agcatcttct aaagaaatcc tatccagaat
    27721 ttcttggctt tggggagaaa caatcgcact aaactgactt tttaaaggtc tggaggagaa
    27781 ataagcatcc gattgctcgc tactgatttt ttttactcga ccacgaacac gtacttgtct
    27841 tgccatttga ggccaataaa aattaagtgc ggcataagga ttcttttgga tttgcattgc
    27901 cttggcactt tgataattgg tataaaagat aaaattaccg ttctctaatc cttttaataa
    27961 gacaactctg gaatctggat agcccttttc atctactgta gacagaacca ttgccgtggg
    28021 atcatttttt tcatttagta atacatcatc aaaccatagt ttgaattgag aaatgggatc
    28081 attttcagcg gattcttcac tgagctgcaa ctcaccataa tctcgtctaa tatccgcaag
    28141 acttctaaat ttgctcatag caaaccttca ctaaaaaaat gtaaataatt atactctcta
    28201 aaatttataa tttgcgacac attttacagt taaaattttt caatcgactt cttgcataaa
    28261 ctgattattt tcaggactta tgagaacccc catggccaag gcaaaagtgc ttttaatgag
    28321 ggaatatagc aataaactaa ataactaaat taaaaaaaat tttaacaaag agattgtggg
    28381 gtcctaagac ctgattttgt tttagggcag gttatgaaat ggatctaatc ttatttaacc
    28441 aagtgacaat attatcatga tggctgttgg gctgcgcttg atacacagca ccaacatgat
    28501 tcgagggaaa atcaccaatt tattttaatc agttcctatt tctataatgc aagggctgtt
    28561 aagtaagaaa aagtattttc ttaccaacca gttacctcag ctattgcgtc tcccaagtca
    28621 gcaggagatc gaactgtttt cacaccagcc gcttccagag cagcatattt ttctgctgcg
    28681 gtccccttcc caccagagat aattgctcca gcatgcccca tacgctttcc agcaggtgct
    28741 gtgactccag cgatgtatga tactacaggt ttttttacat gagacttaat gaattcagct
    28801 gcctcttcct ctgctgaacc accaatttct cctaccatga tgatagcttc tgttttagga
    28861 tctttttcaa ataaagacaa agcatcaata aagctggttc ctggaattgg atcgccacct
    28921 attcctatgc aagtactttg accaaaccct ttatcagtag tttgttttac tgcttcgtag
    28981 gttaaagttc ctgaacgtga aacaattcca acttttccgg gtaaatggat atagccaggc
    29041 ataataccta tcttgcattc gcctggtgta attattcctg gacaatttgg accaattaat
    29101 cgagcctgtg tattgttttc tatatacact tttacttgca acatatccaa tacaggcaca
    29161 ccttctgtaa tacaaacaat cagtttaaca cctgcctctg ctgcttcaat aatggaatct
    29221 ttgcaaaatg gtgcaggaac atagataacg cttgcatccg cctgggtttc ctcaactgct
    29281 tcacgcatag tattaaatac aggcaacccc agatgcattt gaccaccctt acctggagta
    29341 actcccccca ccatcttagt cccataagcc agcgcttgct ctgtatgata agtaccttgt
    29401 tttccagtaa aaccctgaca tagtactttt gtatgtttat caactaatac actcattgtt
    29461 aacctcgtaa tttaatggca tatccgcttt gaaaactacc ttaccaataa tgccaaaatt
    29521 aatttattct ttattcggaa actgctgcta caattttctt ggctgcgtct gtcaaactcg
    29581 ttgcagcaat cacatttaaa ttgcttttat tcaatatctc agcacctaac tgggcattat
    29641 taccttccag tctgacaaca acaggaattt ttacatccac ttcttttaca gcagcaagaa
    29701 ttccatcagc aataaggtca caacgaacga ttccaccaaa aatattaact agaattcctt
    29761 tgactttttc atctgacaca ataatcttca aagcttcgct gactctttct tttgtggcgc
    29821 ctcccccaac atccaggaaa ttagccggct caccaccatg taattttatc acgtccatgg
    29881 tagccatagc caaaccagca ccatttacca tgcaacctat tgtcccatcc aaaggaatat
    29941 aattaagttc ccaatcactg gcacgatttt ctcgatcatc ttcctgggaa acgtctcgca
    30001 tatttttcaa tttaggctgt cggaataaag catttccatc gatattaatt tttccatcca
    30061 gacaaatcag ttgccctgat tttgttatga caagcggatt tatctctaat aaacttaaat
    30121 cacaatcaac aaacattttc cctaatccca tcatcaaatg agtgaattgt ttgatttgat
    30181 cgtcttttaa tccaagttta aaagctgttt cacgacattg aaagggcata acaccaacta
    30241 aggggtcaac aaccaccttg aaaattttct caggtgtttc atgagcaacc ttttcaattt
    30301 caacaccacc ttctgtggag gccataatga caactcgtct ggtggctcta tcaacaacag
    30361 caccaagata taattccttg tctatttcgc aggtttcttc aaccaaaatg gcattaactg
    30421 gttgacctct ggcatcagtt tgataagtaa ccagacgtgt gcctaacatg gatttcgcaa
    30481 ctgcagccaa ttcatctttg ctagaaacca gtttcacacc acctgcttta cctcttccac
    30541 cagcatggac ttgtgctttc acaacccatc gagaggttga taattgagaa gcaacgagca
    30601 aggcatcttc aacattataa gcgacctcac cacgaggaac tggtaaacca tagctagcga
    30661 acaattgctt ggcttgatat tcatgtaaat tcattctaat taccttttaa aaaagcaaga
    30721 cccgtcaaga cgggtcttgc agttaaacat gactcattaa acattcagta aaagtcggga
    30781 tggatcttcc aacagctctt taacactcac taaaaattgt acagaatctt ttccatcaat
    30841 taaacgatga tcataggata aagcaacata catcatagga cggattacta tttgcccttt
    30901 ctctacaata ggtctttcct caattttatg catccctaaa ataccagttt gcggtgggtt
    30961 gataatggga gtagctaata gtgaaccgaa gactccaccg ttagttatgg taaatgtccc
    31021 cccttgcatc tcttccatag ataattttcc ggttctggct tttgatgcag cgtcattaat
    31081 ggctaattca atttctgcca tacttaactg atcagcgtct ctaatcacag gaacgactaa
    31141 acctctttca gtagaaacag caattcctat atcatagaag ccatgataaa caatatcttg
    31201 gccatcaatt gaagcattta cagccggaaa tcgtttaagc gactccacta ccgccttcgt
    31261 aaaaaaagac ataaatccaa gttttacacc atgttttttt tcaaagctgt ctttatactg
    31321 agaacgcata tccataactg cctttaaatt cacttcgtta aaagtggtta acatggcagc
    31381 attgtgctgt gcttgtaata aacgttccgc aatcttggct cttagtctcg tcatagggac
    31441 acgtttttct tccctgccac caaaagaaag tgttctcata gaagattctt tttggacttc
    31501 agcagtttgg tttgattttt ctctattgga ttctatgtaa gataaaacat cttctttggt
    31561 tatcctgcca tctttaccgg taccttgaat ctgcccggct ttcaaatcat tttcagccat
    31621 catccgtctt actaccggac tggttgattt atcttctttt gtgctaactg attcactttc
    31681 ggaaattttt atttcttctt ttttgacagg tttctcttct ttctcagttt tttgagattc
    31741 tacagaaaca ctttgagaaa tttttgccag taagtctcct gaaccaaccg tatctccggt
    31801 attaaacaga atgtcagaca ataccccatc cacaggtgat ggaacttcaa gcacaacttt
    31861 atctgtttcc agatcaacta ggttctcatc gcgagatact ttatcaccca ctttcttatg
    31921 ccatgcagcc actgttgcat cagctactga ctcgggtaga acaggtactt tgacttcaat
    31981 agacatggtt ataccttctc ttaatattat attgtttaga tttatttttt accaagtaat
    32041 gcttgctcaa ccaaagcaac ttgttgctct gcatgcaacc cgggactacc aactgcaggt
    32101 gcagccgcaa actctctgcc agcatattgc aaggtttggt cagaacgcaa gcaatcacgt
    32161 atattatgct gagaagagaa ccagactcct tggttttgtg gctcttcctg acaccagaca
    32221 acttcattag ccttttggta tttctctaat tcagccatta atgctttttt gggaaatgga
    32281 tacaattgct ctatacggac aatcgctacg tggtttaagt ttttatcgcg acgcatttgt
    32341 aataaatcat aataaacctt accacagcat agcaccacct ttgttacttt ttgtgcgtcc
    32401 aaggcatcga tttccggaat tactgtatga aactttcctt taaacaaagc ttccaagggt
    32461 gaaacagcca atttatgtcg taaaagactt ttgggagtca tgacaattaa aggcttcctg
    32521 aaattacgaa taacttgtct tctcaacaaa tgaaatattt gtgctggagt tgttggagtg
    32581 catacttgaa tattgtgctg tgcacaaagc tgcatataac gctccaatcg cgcagaagag
    32641 tgctcagggc cttgtccttc atagccatgg ggtaatagca tcaccaatcc gcacagccgc
    32701 ccccactttt gctcaccgga acttataaat tgatcgataa cgacttgtgc tccatttgca
    32761 aaatcaccaa attgagcttc ccaaataacc aggaaagatg gctcagacga agcaaagcca
    32821 tattcgaaag ctaataccgc ttcttcggaa agaactgaat ctatcacggt aaaatagcgc
    32881 ttgggattgg ttgatatatg ttccaaagga ataaaagtat ctcctttttc aatatcatgg
    32941 agtgcagcat gtctatgggc aaaagttcct cgaccactat cttgtccaga aattcttacg
    33001 ccatagcctt catgcaataa gctggcataa gccatgatct cagcataacc ccaattcata
    33061 gggatttcac cagcggtcat tttatttcgt tcagctaaca aacgctccac aacgggatgc
    33121 agtttcattc catcaggcaa tttgtttaat tgggatgaaa ttttttccaa ctcttccttg
    33181 ctaatggttg tgtctacttt atccgtccac ttggcattta tataaggagt ccaatctaaa
    33241 gattgctttc cttcataatc ttcatgaacc aaatcgacaa ccgccttgcc tttatctagt
    33301 gcttctctgt aggaatcgac caatttgtct aattctgttt tagttaataa attaacactg
    33361 accaattgtt ctccatattt ttcacgtaat gtaggcatgg atttgatttt tttatacatc
    33421 tttggctggg tcactgaagg ttcatcagct tcgttgtggc catgacgtct gtaacagaca
    33481 aggtcaatta ctatgtctcg tttaaacttc atgcggaaat caaaagcgac ttgagtagcg
    33541 aaaactactg cctcaggatc atctccattg acatgaataa ctggcgcctg aaccattttg
    33601 gcaacgtctg tacaatagag agttgaacgg gcatccaaag gattgctcgt tgtaaatcct
    33661 atctgattat tgataacaat atggatcgtt ccaccagtac aataaccacg tgcctgggag
    33721 aagttaaagg tttccataac cacgccctga cctgcaaaag cagcatcacc atgaatgaca
    33781 atgggaacta ctttatcttt tttagccaaa tcgttgcgtc ttcccaaacg tgaacgtacg
    33841 gatccctcaa ctactggccc aataatttcc aagtgtgaag ggttaaacgc caaagccagg
    33901 tgcacaatag cacctgattc tgtttttatg tcagaggaaa aacccagatg atatttcaca
    33961 tcgccagttc gttcgtattt aatttttcct tcaaattctt gaaataatag tcctggttct
    34021 ttaccgagaa cattcactaa cacatttaag cgccctctat gtgccattcc aatcacaagc
    34081 tctttaacct gatttacacc agatcttcta atgagttctt tcatcattgg aattaaagaa
    34141 tctcccccct caagcgaaaa tcgtttttga ccaacatatc gggttcctag ataacgctcc
    34201 aaaccgtctg ctgctatcaa atctttaagg atattcaatt tttcctgctt actaaattca
    34261 ggacagccac gaactgattc catcttatgt tgtaaccatt cagtgacttc agtatcggat
    34321 atatgcatat actcaatgcc tatacttcga cagtatgttt cgcgaagggc gttgtatatt
    34381 tcccccaaag tcatctccgg cccattaaag ctggtaccag caaaaaaagt gcgattaaca
    34441 tcaacatcgg caagattatg ataagcaagc tccagccgcg gtactggcat gcgttccgtc
    34501 atttccaatg gatccagttt tgcggcaaga tgccctaatg atctaaaatc attaatcaaa
    34561 ctggcaacct ggtactgttg actatctgca atttgaatta ttttattaag cttcttgtca
    34621 gcattctgca aaaaataatc gcgaatgtct ctatgagata cttctttggt agcaccattt
    34681 actttgggta aagcgctaaa taccgctctc caatcctctg acactgaatc agggtctgcg
    34741 aggtaatcct cataaagact atcaacataa gccatacttc ctccagacaa ataggaagaa
    34801 gcccattctt tttgcagatc agaactgctc attatcattc tctccaaagg gttcactcag
    34861 aaactttctg agtaacaagg actgatttat tacgtttcct gcgtcaacat ttgcgtgcga
    34921 atttttgcaa tcgcttgcgt tggattgagt cccttcgggc atacattcgt gcaattcata
    34981 atagtacgac aacgaaatac actgaaagga tcttgtaata aatccaaacg atgttgtgtt
    35041 gccttgtcac gactgtctgc taaaaaacgc ctagcttgta aaagtccagc agggcctaca
    35101 aatttatcag gattccacca ataagaagga catgaactgg tacagcaagc gcataaaatg
    35161 cactcataca agccatccaa ttgtgcccgc tcttcaggag attgtaacct ctctcgagca
    35221 ggtgctactt catcattctg taaataaggc tcaatgcgtt catactgctg ataaaattgt
    35281 gtcatatcaa ctgccaagtc acgaattact ggaaaacctg gcaaaggacg aataacaatt
    35341 ttatctgttt ttaattgtga aagatgtgta atgcaagcta acccattagt cccattgata
    35401 ttcataccgt cagagccaca tactccttct cggcatgacc gtctgtaagt aatagaaggg
    35461 tcttgctcag ctttcaagcg ctcaagcaag gtaagtaaca tgggatcact tttagccggg
    35521 atttctatct cataatcttt catgtaaggc ttggcatcca cctcaggatt atagcgataa
    35581 atcgacaaga ttacatttct actatcagcc ataaaatatc ctcattagta aacgcgttct
    35641 ttcggctcaa aaggctcgac atacttaggt gaggtattaa ccggacgata atctatcacc
    35701 tcaccttcct caaaatacaa tgtgtgctta atccagtttg catcatctcg ttttggataa
    35761 tcttccctgc tgtgcgctcc tctgctttca gttcgcgcaa ttgcagataa agccgttgca
    35821 taagcagttg ccattaaatt atccaattcc atagcttcta cgcgctctgt attaaaaatt
    35881 tgacttttat catcaagttt agcatgtgct aatcgatcgc gcagggcttg taaacgtttc
    35941 agtcctgaag ccattacctc accggtacgg aatactccaa aatcttcctg cattactctt
    36001 tgcatttcat ctcgtattac cgcatggctt tcaccatcct ttgagttttc ccaacgctca
    36061 taacgaacca gagaagctac tacatcgtct tcacttacat aagacatatc aggcaattga
    36121 tttgattgcc ataattcttc aacatgtatc ccggctgcac ggccaaacac aaccaaatcc
    36181 aataaggaat taccacctaa tcgattggca ccatgcactg aaacgcaagc acattcccct
    36241 acagcataca acccctccac cacatgctct ttgccatcag ttttagttat aacctggcca
    36301 tgcatgttgg tgggtattcc tcccatactg tagtggcaag ttggaacaac tggaatcggc
    36361 tcaacgattg gatccacacc cgcaaatttc attgataact cgcgaatgcc aggcaagcga
    36421 gacataatca aatcagcgcc cagatggtca agctttaact taacatgatc gacacctttt
    36481 ggatcaaatc ctttcccagc ccgaatttcc aaagccatag cgcgagcgac cacatcccgg
    36541 gaagccaaat cctttactct gggagcataa cgctccataa aacgctcacc atctttgttt
    36601 attaaataac caccttcacc tcggcaaccc tccgtaacca gaacaccagc tcccgctatg
    36661 ccagttggat gaaattgcca catttccata tcttgaagag gtataccagc tcttagtgcc
    36721 attccaaagc catcgccagt attaataaaa gcattcgttg tagattgaaa aattctaccg
    36781 gctcctccag tcgccaaaat acaaactcga gtctgaaaaa atacaatctc gccagtctcc
    36841 atacacatgg cagtaacacc tgcaattctt ccttgcgagt tttttaccag atcgagagca
    36901 taccattcac taaatacatg agttttagct tttaaattct gttgataaag agtgtgcagc
    36961 aaagcatgcc ctgttctatc tgccgcagca caggtacggg ccgcttgttc tccaccaaag
    37021 tgttttgatt ggccaccaaa ctgacgttgg tagatttttc cattatccat tcgtgaaaaa
    37081 ggtagcccca tatgctccag ttcataaact gcttcaggcc cggttttaca tagatactct
    37141 atacaatcct gatcaccaat gtaatcagca cctttcacgg tatcatacat gtgccagcgc
    37201 cagtcgtctt catcagcatt tcccaaagcg gctgtaatac caccttgagc cgacacagta
    37261 tgtgagcggg ttggaaatac ttttgataac aacgctactt ttaaaccaga atttgtcatc
    37321 tggagtgcag ctcgcattcc tgcaccgcca gctcctataa ttaccgcatc aaatctgtta
    37381 cgtgccactg tcatgctatt gtccccaaat gatcataagg ccccagataa attgaccaag
    37441 gagccataaa acaaccaata actgtactga taaacgtata acagtacatt tcatataatc
    37501 tgtagttact gtccaaatac ctatccaggc atgcaatgtt aaagccatca acccaattgt
    37561 tgttgcaatt tgaaatagct tattggcaaa caaaagatgc cattgagaaa agtttaaatc
    37621 aggatttgcc aatataaatc caaataaaaa aattaaatag gctgcaaaat agacagcagt
    37681 aactctttga attaaccaat cctttaaccc atttcctgtt aagctggtga cattgtttac
    37741 catatccaga ttcctagaaa aattgccaaa ataactgcaa gagctataat tgaaacagcc
    37801 gacatacgac cagcacgaag actctcacca aatcccatat ccattatcag atgtcttata
    37861 cctgcgatga catgataaat catagaggcg ccaaaagccc ataacagaag tttatagtag
    37921 ggattagcta aaattatttt agtctgtgca aaagattcct ctgattgcaa ggaacaacca
    37981 agaataaaca atacgaaggg aaataataaa aatataagaa ctcctgaaac cctatgtaat
    38041 atcgatgcga tagccatagg cggaaatttc aaactgctta aatcaagatt gatcggtctt
    38101 ttcttgttca ctattaattt cttcctcata agaaaatgtc atcaatatat tttcttctaa
    38161 atattgaatc ccattcaatt atttcacatt caccctttag acttattgag aaacataatt
    38221 cattaagtat actgcgcaac aaatggcttt gaaaaataaa aaattgggtc ttaaaaacta
    38281 tttagaatta ctaaatttca attcatgcat gggaagtata gcactctgtt tttatcacgc
    38341 aaagttcagt ttataaggaa aatgaatagt ccattggcca attacaaaaa cccgtactat
    38401 gattacataa tatgatcaaa aggagatctt tatgatcgcc aaaagaccaa tatataatca
    38461 acaattaaaa tgtgtcgcct ttgaaatatt atcctgtcgc aatgtaatac ttaatgaaga
    38521 attaacaagg caattttatg agctcattta ccacacagac acccaattac ctttatttgt
    38581 tcctttttcc ctaaaaaaat tgcttgagca atttgagacg ccaatcaaca accccattat
    38641 tttaaaatta cccgcagatg aaatcgaatc ggtttattca ctaactgagc tacaagactc
    38701 tctattttct attgccttac tgattaatac ccctcaacaa ttggcttggc taaattttgc
    38761 cgaatacata ggattgactg agcagttaat gaataaatcg gatgtaacca aggtagtaca
    38821 atacagtaag gcaaaaaatc gtaaaatcat tgcttatgat ctaaatcaac acgttaattt
    38881 tgaaaaatgt agggctatga cgatggatta ttactgtggc gatttcctgc ttaactacat
    38941 tgcagaggag cacacagaaa tagcagcaaa caaattaaat gtacttcaac tcatccagga
    39001 attacaaaaa gaagattgcg atttaaacac gatcactcat atcatccaag aagatccatt
    39061 actaagctat caacttctaa aactggtcaa ttcagttgga ttttctagtg gaccaagcat
    39121 agaatccatc gatcaagccg ttgccaggct tggcaccatt aacctgaaaa aatgggttat
    39181 gctgttttca atgaaaaata tctcagataa acctgtagaa atattggagt caggtctaat
    39241 cagagctcat atggcggaag agctcgctaa aaaaagttcg aatattttca gccaaagtgc
    39301 ctatactgca ggattattat ccatattaga ctgcttatta aacaaaccca tgccagcatt
    39361 ggtagaacaa atcaccctag ctgaggagat aaaaaatgct ctgacaaata aatcaggacc
    39421 attaggaaat ctattgtctt tggtaattgc ttatgaagaa gggcattggg aagaagtaac
    39481 tcaacttaac atcaatggaa ttgatttaag tcaattatat attgacagtt tagccttggt
    39541 aagccaaggt acaaaaatca tgagtaactg attgcgcaac aatttaaaca atattcattt
    39601 tatttttcta tgcctgaccc aggcttctat ttgagcaaca aaaaatttag aatgtgtttt
    39661 cctaaatgat taatattcca aaatttaata gttgaaatca ataaaggata tgtattagtt
    39721 atgtttttca tcaaatactt aggtgcattg atttatgatt taatcatctt attcatttta
    39781 ttttttgcat acacctctgt cctgctatta ttcacccatg gacaagccat acctcctgcc
    39841 acccgttggt atcaattttc attactcagt atcactcttg tttattatta tggttc
//
LOCUS       DACTJE010000008        39441 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.008, whole genome shotgun sequence.
ACCESSION   DACTJE010000008 DACTJE010000000
VERSION     DACTJE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 39441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39441
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.008"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            169..696
                     /gene="aroK"
                     /locus_tag="I9760_000312"
     CDS             169..696
                     /gene="aroK"
                     /locus_tag="I9760_000312"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAT5042107.1"
                     /translation="MSIVKVRNIFLIGPMGAGKSTIGRALAKELKLEFYDSDEVIEER
                     AGADISWIFDIEGEEGFRRREQKVIDELTQKTNIVLATGGGVVITPENRNALAGRGTV
                     IYLKTSLQQQFERTKRDTKRPLLQTEDLEGRLESLRDEREPFYDELADVSFETDKLTV
                     KAVANNIIKYLYGEV"
     gene            683..1792
                     /locus_tag="I9760_000313"
     CDS             683..1792
                     /locus_tag="I9760_000313"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAT5042108.1"
                     /translation="MAKFELYAEVDVSISGHQYPIIICRNGLIDPDLINRFITSKQVL
                     IVTNRTVAPLYLGHLQSVLPSKQCDVVILEDGEEHKNQRSLFTIYDSLIQNKHHRDTS
                     IIALGGGVIGDMAGFAASTYQRGVRFIQLPTTLLAQVDASVGGKTAINHPAGKNMIGS
                     FYQPQAVIIDLNTLKTLPEREFRAGIAEMIKYALLVGGSFFERIQDVLQQGLTVHSPE
                     LPLLIAECCQVKAKIVEQDERESGLRALLNLGHTFAHALETYTDYKKWLHGEAVAIGL
                     YCAAVLSEKKGLLDNLIVDQVEKMLIHAGLPHKIPNSIDLIQLRELMSLDKKIKNNCL
                     RFIMIKKPGACYIDDSVTEDCLHNTLINVVEGEQK"
     gene            1789..3240
                     /locus_tag="I9760_000314"
     CDS             1789..3240
                     /locus_tag="I9760_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042109.1"
                     /translation="MIDGKEPLDEGVANQPRTLFKPGAWLAKVDFINHLILFNNVLIT
                     ILSEKEGGKTCFNSLLKSHLNQQIKSVSFKAKPPFDSQDIINEFARQLHLNNKDNNIG
                     SLAAQINERKTHVLLIIDDAHHLPESFIKEVMIIIKNQEDFGFFHLCLISDYSIVATL
                     NNLAVEPFNNLIHTIELGALSETETRTYVLQRAMASHLINRPLTDTQFKQFYHLTKGN
                     LSKINSNLELFLSKCANEKQNNKKAILKRISLVASFAIVAGLSYFYFDNVYKAPDNTL
                     KLPDLTSVTTLPPINDLIKTPTQQAVPKQESVSYIASWEDSSTRQLVHYGLPKKQILD
                     DFMDEEDSLNTVAVVDKVVVIPKLKAKNISEQVGQAVVSQSKLPPIPHSSQTKLASEQ
                     PGIKKINSKLFTIQLAASHKVEDINRFKNQNKWLAQAKIRHFTNAKGSWYILTIGEYE
                     SRNQALLNIKKLPGELAKLSPWIRSVEGLSNIG"
     gene            3349..3780
                     /locus_tag="I9760_000315"
     CDS             3349..3780
                     /locus_tag="I9760_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAT5042110.1"
                     /translation="MYKRVLFATDFDEVGVRAAEKAKKIADENGADLILVHVVEPIPA
                     YAYPGFAGFAEVEMSIREQAEKELNELAEKLGVDEKHRFIEYGSTKNEILRVAKEHKI
                     DLIVTGSHGKHGLSLLLGSTANAILHGAECDVLIVRPKISH"
     gene            3904..4890
                     /gene="ribF"
                     /locus_tag="I9760_000316"
     CDS             3904..4890
                     /gene="ribF"
                     /locus_tag="I9760_000316"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAT5042111.1"
                     /translation="MKLLRSSTHFAIFDKGAVATIGNFDGVHLGHQHLIKALRAKADE
                     MNLPLVILLFEPQPKEYFHREKAPARLSTLREKIDVLNLCQVDYIYCIKFDARLAQTP
                     ALYFAQFYLFEALKIRYLLVGQDFRFGKNREGDVNLLKTLGANYSCEVTVQSDFLIEN
                     EKISSTRIREALQQGNLRFAAKLLGRPYSMCGRVIKGDGRGRQWGIPTANLGIHRLSI
                     PLQGVYAVQVHINSNVFYGVANIGKRPTVAGTKNILEVHLFDFHGSLYGQLVQVIFLH
                     KLRDEVKFSSVEALITQINSDIILAKTYLKQNWLDSAIDSSNDVVNGISNTV"
     gene            5003..7798
                     /gene="ileS"
                     /locus_tag="I9760_000317"
     CDS             5003..7798
                     /gene="ileS"
                     /locus_tag="I9760_000317"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAT5042112.1"
                     /translation="MAEYKDTLNLPNTSFPMKASLSVREPEMLADWQAKGIYKKIRKA
                     RDGSKRFILHDGPPYANGHLHCGHALNKILKDIIIKSKTFSGFDAPFVPGWDCHGLPI
                     ELNVEKKVGKAGSKISPREFRAKCREYAASQIDIQRDEFQRLGVLGDWYNPYVTMDYH
                     YEANIVRALGLMIKNGHLQQGFKPVHWCIDCGSALAEAEVDYEDKTSPSIDVAFSAVN
                     PSEFLNCFETQPAVKPLILPIWTTTPWTLPANEAVCLHPEIDYALIDAGNSYYIVATD
                     LVESVMARYGISHYKTSGSAKGRVFEHFKLQHPFYKRQVPVVLAEHVTTESGTGCVHT
                     APAHGPDDYLVGQFYQLPLINPVMANGCFAENVELFAGISVLKANETILAVLSERNVL
                     LASESIRHSYPHCWRHKSPMIFLATPQWFISMDKSNLRQAIINEIDKVNWVPDWGKAR
                     ISNMVENRPDWCISRQRSWGTPMPLFVHKTTRELHPDTLELIERVAMMIEKSGIDAWF
                     DLDSSELLGDDAKHYDKITDTMDVWLDSGISHYSVLKHNNDLDFPADVYFEGSDQHRG
                     WFNSSLTTAVAMYGVAPYKTVLTHGYTVDAEGKKLSKSKGNYVALDKLVNQHGADILR
                     LWVASTDYRHEVSISEEIIKRNADAYRRIRNTARFLLANLFDFNPASDCIEAKELLEL
                     DRWALKRCQLLQEEIITAYENYHFHLIYQKIHNFCAVDMGSFYLDLIKDRQYTTAKDS
                     IARRSCQTAMYHMVKAFTIWLAPILSFTAEEIWQTIPGNNSESIFIEHWYNAWPTIDA
                     VNMEDWEQLHIVRDEVNKALEETRQRGEIGSALAAEVTVYADAKVLPKLTRLGEELRF
                     LFITSEAKACPISQSPKGLAVTDCGVSIQVTASVHEKCARCWHRREDVGQNQEHPELC
                     LRCVGNISGYHEERLYI"
     gene            7795..8259
                     /gene="lspA"
                     /locus_tag="I9760_000318"
     CDS             7795..8259
                     /gene="lspA"
                     /locus_tag="I9760_000318"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAT5042113.1"
                     /translation="MKKWPWLVLSFLVIICDQLSKYWVGVLLTPYKPMPVMPMLNFTL
                     AFNTGAAFSFLSGAGDWHRWFFAGFSFLMSIILLIWLVRTPEQARFQSVGISLILGGA
                     IGNLIDRGLHGYVIDFIDVYYKHHHFATFNLADSAICIGAAILVLDLLIRRE"
     gene            complement(8556..9119)
                     /locus_tag="I9760_000319"
     CDS             complement(8556..9119)
                     /locus_tag="I9760_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HAT5042114.1"
                     /translation="MIIEKILGNQSVIISLDIDNFLFDRLDQIANSDLDLVEINSSDT
                     NLLVKIKTQYPSLKIGAGGIIDTQQLEQCYQAGVHFASSPGFLASIAQTASVYSMCYF
                     PSVATISEAMAAANIGFKQVRPYPANLTFCNALSKYLPGLNLFPAEIDWNEAELFLNL
                     PAVAAVIIHNPDHKQLNGLARLAQSII"
     gene            complement(9295..11502)
                     /gene="lidA"
                     /locus_tag="I9760_000320"
     CDS             complement(9295..11502)
                     /gene="lidA"
                     /locus_tag="I9760_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LidA"
                     /protein_id="HAT5042115.1"
                     /translation="MAKDNKSHQVKTSEGSLESVKTKEKEPVVEKMRVEDSKKEDKLS
                     MPTTKKESQPNEPAKPFKTSFEKWIESSLLDPQAKEDKGSTINLGREGLKNASQVKKF
                     LLSPAGKDVIAELGAQMALQRNINLQNQQDRMEHELFKRRLMAALFLWYLSKKSHAAE
                     KVKEIIREYNEKAIKNAEKASKPSQQSTSSTSQADKEIQKMLDEYEQAIKRAQENIKK
                     GEELEKKLDKLERQGKDLEDKYKTYEENLEGFEKLLTDSEELSLSEINEKMKAFSKDS
                     EKLTQLMEKHKGDEKTVQSLQREHHDIKAKLANLQVLHDAHTGKKSYVNEKGNPVSSL
                     KDAHLAINKDQEVVEHKGQFYLLQKGQWDAIKNDPAALEKAQKDYSQSKHDLATIKME
                     ALIHKLSLEMEKQLETINDLIMSTDPKENEEATKLLHKHNGLNLKLANLQDMLAVHRK
                     EKSFFNEKGEKVTSLNDAHYVIGKDQQLFNLGGKFYPIHKEQKILEKDGKFYLLKQGE
                     DWESIKDSPEKQKKAEHDFHKLQYETPMTVKKLVHHNKGLETTIHKERIEETKQQLED
                     NGKEKIEIANNISKLQSTVGVALNQLNQPTLDTESPVLTPSGGSTTVSPKPTPSLASV
                     TTFFKTKIDEMKKNEDVKVTLDDIMALSIKIADPKARTYFKNEVLKFAEQLKEVPRSG
                     AIPFQTMQRMLQTLEQYGVDTTKPSVTSIRSKTDEVLEQRFNPTPSLSPFKTS"
     gene            complement(11744..14869)
                     /locus_tag="I9760_000321"
     CDS             complement(11744..14869)
                     /locus_tag="I9760_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042116.1"
                     /translation="MPDFETTDGQTQVIIPLRVTPHTHKAMPTITGSIFSHEAGTVFY
                     GNYNMEYLLPGGDRLRLIANHNKKTVQLMLFTHNPELLKILPGDRPSSLVADVINHIN
                     RYKVQAESYKKPLSHEQEEKINLANACIRSLNHLLKKERQLKPHDHAGQEELRRKIIS
                     IIEHCQNDNRMIANNPVVSEGSFGYMLYDAHKTAQHYRFNRVFSVSRQDQMDFTKVTK
                     TQLGKPITPCFVWDSEIHIGHSSSELDDALRVICQQYQLCPAPALNNIPANRFAKLER
                     FISKLWHDGHDWINYLAITTKPSHKTEIQKRSDGVSITKITPYYKLKGLPQRGYNSLA
                     AMVNHLVENSAQQTEVNSLKEAKRLLSHLPNGNWVIVPQKNTVLLRLENKLIHLKYFI
                     HDQLFYPLPDGEDLYILSQISKRHLYLPERISLRFKAFISRIPDFFKGFYKSMSQFII
                     HDLHEEFFNHVHATHAPKQHLSERDQRPKSKKIKRTSLHEALQNQGLLANGQTLEEFI
                     SDQINNSPYVIARANHPPTVHTYENPLHRALGVVRHLAGFFIDTSERNPMIGTLAMAA
                     YVYGAGAVLAPNVLTDILKKLHLSGLIYGIEPVQKLARLMNHGKISEAISASATLWQG
                     MVVSGNLDKFFVDAVSVLKEDPAEIAIIVSLALSLGYGLTKAIPSLQHEMGDFPYANY
                     AALGGKGGAAIYDTIMHPGDDWFLGTCKWFCKNIINIGKLLVAPFFEGFYYGFFDGFI
                     NGWKKSGKLIKQLGKQTLAASFDLILAILTIPLLEASSLLIHVPFRGITNFFRKLLSV
                     LGNFSVIGKLLIDIAERPSTNNFISEFTLSPLYGFTSPFGHFSDNPVTNIGINVIRLL
                     FLPLLQLIKNILILPLIDILSLTIRVNLSIINPISRLLAYSTGTILYHFGQIWDHSAG
                     VLFSTSAYGVTILCNHIDSKAGELKQYLLSLIEINRGKVYQWAFHDEDILTHKIVQDN
                     QYYFNDPRRCELIPHSDSHCLLRNLLLDEKSSTNKVKDNQKSQQLHFPGLFHGATQTS
                     DTMPQEHTLTI"
     gene            15131..16018
                     /locus_tag="I9760_000322"
     CDS             15131..16018
                     /locus_tag="I9760_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAT5042117.1"
                     /translation="MSSYCGYIALVGRPNVGKSTLLNRILQQKLSITSRKPQTTRHSI
                     LGIRTEGEFQFVYVDTPGIHQGNAKAINRMMNKTAISVLRDVDVIAFLVDGTHWKDED
                     EYVLNLIKQAKVPCILVVNKVDKITDKTQLLPWIEQISQRYQFAAIIPLSAKTGLQVD
                     ELEGKLKNYLPEGPHLFPDDQFTDRSTKFLCAELLREKIFRFCGQELPYSVTVDIESF
                     KDEGNLIRIHALILVDKDNHKRMIIGDKGQKLKEMATNARLDMEKMLDKKVFLQCWCK
                     VKSGWSDDERILKQLGYDQ"
     gene            16008..16697
                     /gene="recO"
                     /locus_tag="I9760_000323"
     CDS             16008..16697
                     /gene="recO"
                     /locus_tag="I9760_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAT5042118.1"
                     /translation="MTSKSLNAWVIHKQWSGDTSARLKLFTRELGLINCLCKGGRTPK
                     KQSLLQAFIPLWVSIEERYDQYYTRNIESTSSRLDLEGHSLFSGLYINELLYYTLSPD
                     FPDPDLFDAYLFTLNGIALAREREAIEALLRRFEWALLKACGYTFSFLHEARTGELIV
                     PDSYYQFVAGEGFILGGDKEIPGEHLLAIAADNLSESAYLKSAKFIMRQAINHLLGGR
                     EIKARSLYGPA"
     gene            complement(16753..17928)
                     /gene="ravJ"
                     /locus_tag="I9760_000324"
     CDS             complement(16753..17928)
                     /gene="ravJ"
                     /locus_tag="I9760_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavJ"
                     /protein_id="HAT5042119.1"
                     /translation="MAIAPQQIQERLKQEQYQKFVVADIGNFPHCLAQTPEGIASGQR
                     YQKYSTNSLSRTPPFSQWGAPQLLTPKSAQEYIKFAQQRNKKSSFKIDGEAVRVSECS
                     NFAYHSAGVLLDDPQIRTQYDVAVIGSMHSNGRYLHNITLLVPKGSRLPQPPQQLTAE
                     VFPIGTLIVDPWAVGMGHPPEQALAIPKEQFAYNRSLFPATVNYQSALDESLTSTRTG
                     QLTPYTGTPSRTGRQEVRAESQFPGTRRPVSATIPGNFAAYHELWRNAFQEIMNDPRH
                     QLHRNDVEYKKIHAIRTVLDDYTKGGNTWWAKFRRIFTFHWNRHHVKVVDDIVKEIDA
                     GNYTTSRALVDRLDNLAISLGSKLNPKGTLKEQIGFIKIQEQNPVEEIENLSRPNLT"
     gene            complement(18091..18981)
                     /gene="legL1"
                     /locus_tag="I9760_000325"
     CDS             complement(18091..18981)
                     /gene="legL1"
                     /locus_tag="I9760_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL1"
                     /protein_id="HAT5042120.1"
                     /translation="MVLTLSDLEKGYDKNLNQLSLSFLNLRDNDIPLLCEFLQNHPAI
                     TSLDLSHNDITANGVKLFVNKTSVSSLNISHNNIGPEGAQWLSEDNHITTLDVSFNEI
                     GDEGVKALAANAKLITLYALYNKITKVGAGYLAQSNLKKIDLCFNSLEDEGVIALASN
                     INIKELIASACDVSDIGAIELAKNNQLTLLILGKNAITDKSTLHFANNTSLSTLHLGS
                     NQITAAGKKILETNTRITDLDLIGNPIEVHETDELNYSKKSPDSHSVFKFLQKFTFLS
                     CMSPCQETAESDKGLNKKPN"
     gene            19270..20016
                     /gene="pdxJ"
                     /locus_tag="I9760_000326"
     CDS             19270..20016
                     /gene="pdxJ"
                     /locus_tag="I9760_000326"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAT5042121.1"
                     /translation="MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGIT
                     LHMREDLRHIQARDVRLIKQVLQTRMNLELAVTEAMLDFAEEISPEHACLVPEKREEL
                     TTEGGLDILTHRNVVEKAVRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCY
                     ADATSEEKQHYELQRIKEAAEFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHA
                     IIARALFCGLKEAVRHMRQLMQEARLYVND"
     gene            20003..21820
                     /locus_tag="I9760_000327"
     CDS             20003..21820
                     /locus_tag="I9760_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HAT5042122.1"
                     /translation="MSTTDVIVVRITGDSGDGVQLVGEQLTISAALTGRDVRTMPDFP
                     AEIRAPAGTVAGVSGFQLAMAERAIFTAGESLDVLVALNPAALKNSLQHLNQGGLLII
                     NEDSFQERDWQKAGIDKSFLDNCREHYHIVAFPLITQTIQAVESINLTKPQAVKTKNF
                     YILGLVLWLFDLPTETCQAFIAKKFKTNQAIAKANELALLAGYNYAMTLELSRRDYML
                     GEVNRHSGEYRQITGVEAVGLALATLATQTQVPLLVSGYPITPASAILHECARLSDYG
                     VQLIQAEDEIAAICSCIGAAYGGRLALTCTSGPGLDLKCESLGLAVVTELPLVLVDVQ
                     RAGASTGLPTKTSQSDLKQALYGRHGEAPLPVIAAQSPADCFNTIIEAFRIAIKYMTP
                     VIVLLDAYLANAAEPWEIPALDSLNIPKPEFNRFTKPYHRDEFLSRSWNVPGTSGFIH
                     QIGGLEKQGDEGRVSYDAENHQKMVITRAEKIKGIARDYPALVLEGDMTAPTLVIGWG
                     STFGSLKSAVQQCLEQGLPVAYLHLRHLNPLPDDLGSLLKKYDKVLVAELNSGQLCQL
                     IRAVYLVDAQSISQCNGQPFSVNPLVSAIKAEANYEQQL"
     gene            21804..22772
                     /locus_tag="I9760_000328"
     CDS             21804..22772
                     /locus_tag="I9760_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HAT5042123.1"
                     /translation="MNSNYKREDFVNETEVRWCPGCGDYAILAALQRVLPELGLPPEQ
                     HVFVSGIGCAGRLPYYMNTYGFHTIHGRATAVATGLKAMREDLCVWVITGDGDALSIG
                     GNHLLHILRRNVNVNILLFNNQVYGLTKGQFSPTSQKGQVTKTSPKGVTSEPINPIMI
                     ALASGASFVARAVDKDPVHLASVLKKAYEHKGCSFVEIYQDCNIFNHGAFDDFAVKNN
                     RAENTIILEDGQPLVFGAQKEKALQLDDEQFVQISIDEKTLYKHDEKNLISAMRLARL
                     TFPDYPVPLGVYYQKDRERFTLSQEVKKRPEDLPELFKAKASWKQN"
     gene            complement(23101..23775)
                     /locus_tag="I9760_000329"
     CDS             complement(23101..23775)
                     /locus_tag="I9760_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAT5042124.1"
                     /translation="MNRNDITILSQQRESVLATNKVLRNTYLLLSLTFIFSALTAYVA
                     FISGARPMNPLLMIVGVYGLMFLTQALRNSVWGLVSVFAFTGFLGYTIGPLLNYYITG
                     FSNGPQIVATALGGTGMIFFALSGYALTTKKDFSYLGGFLFVGVMVALLAMIAGIFIQ
                     IPALQLVISAAFVLISSGLILLQTSAIIHEGETNYIMATIGLFVSIYNLFVSLLNLLS
                     AFSGRD"
     gene            23994..24800
                     /locus_tag="I9760_000330"
     CDS             23994..24800
                     /locus_tag="I9760_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAT5042125.1"
                     /translation="MNRVALVQMVSSAKIADNLQLTEQYLIEARAQEASLVVLPENFA
                     FMGMNEREKLQIAEYYGQGPIQQRISELARDLRVWIIAGTIPLKSMGSKVRASCIVYD
                     DKGLNVARYDKIHLFDVIVSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFP
                     ELYQYLTQRGAQLLSVPSAFTAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRH
                     TYGHSMVVEPWGKVLAQKEEGQGVILADIDLERLSQLRRQFPCVEHHVLM"
     gene            24812..26254
                     /gene="tldD"
                     /locus_tag="I9760_000331"
     CDS             24812..26254
                     /gene="tldD"
                     /locus_tag="I9760_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAT5042126.1"
                     /translation="MTKSLAIAKNILLTPASLDEVGIEKLFKTMFNRNVDDADLYFQS
                     CSYESWYLEDSEVKSGNFSLDKGVGIRAVSGDKTGFAYCDDILLPSMLRAADAARSIA
                     LSGSKTVPAVKVLKASVNRYESLNPLEGMSKQEKIALLEAIDKEARSLDPRVIQVNAT
                     LTGCYEVVMVANMDGQMMADVRPLVSINVSVIVEDSHGRRESARSGGGGRVAYSYFTE
                     KENALSYAREAVREALTNLKAEPAPAGTMPVVLGPGWPGVLLHEAVGHGLEGDFNRKG
                     LSAFSGRLGQQVAAAGVTVVDDGTLENRRGSLTIDDEGTPSQCTTLIDNGILVNYMQD
                     KLNAKLMGMQSTGNCRRESYAHVPMPRMTNTYMLSGQYDPQEIIRTVKKGLYAVNFGG
                     GQVDITSGQFVFSASEAYLIENGKITKPVKGATLIGNGPDVMKKISMIGNDLALDRGI
                     GVCGKEGQSVPVGVGQPTLKIDALTVGGTK"
     gene            26300..27178
                     /gene="rssA"
                     /locus_tag="I9760_000332"
     CDS             26300..27178
                     /gene="rssA"
                     /locus_tag="I9760_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAT5042127.1"
                     /translation="MTNQYPKIGLALGSGSARGWAHIGVIQSIKRLGVRIDCVAGCSI
                     GALVGAIYACGTLDLFEQWVLKLNKREMAKLIDVNFLGGGVLSGSKLMTFFKDFGLEK
                     NIEELSLPFSSVATDLSTGREIWLSKGSLELAIRSSMSLPGLFTPQRYKDCWLVDGGL
                     VNPVPITLCRAMGAEVVIAVNLNHDLLRVPPSTIEHPGFFSVLSGQFDKMIKRIMGIS
                     DGPGYYDVLTRSIKIMQDRITRARAAGDPADLTLVPQLGNIMLYDFHKAKEAIHEGEN
                     CVLREKNNILQLVGLE"
     gene            27205..28872
                     /locus_tag="I9760_000333"
     CDS             27205..28872
                     /locus_tag="I9760_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HAT5042128.1"
                     /translation="MPKESNSNSLFDLLLKNEKNSADRVYLRQPKNGVWHEYTWSSVM
                     LQARKVAGFLHHLGLKKGDHISIISKNCAEWFITDFGIHIAGMVNVPLFANQNEESAH
                     YVLDHGDVKLVFVGKLDDHQRVRQFIPKNYRTIGFDYHKDLQVDYEWSDVLKSDPMIN
                     LVEPEPDALYTIIYSSGTSGAPKGAMYTNRSIANYLTVFPKDLLRIRELEHYKLISYL
                     PLAHVYERSAIQLGSVAINASVSFVESLEKFAENLREVSPTFFTAVPRIWGVFKQKIE
                     QKLPPAKLNFLLKIPLISSLIKRKIIHSLGLSECTNCFSGASHLPISIIDFFEKLGIY
                     IQEGYGQSENLAYATLSMLNERKRGYVGTPRLEVEIKVGEENELLMKCPCLMSGYYKN
                     EQATKNAFTEEGWLRTGDVVDVDNEDRVKILGRLSENFKNQTGEFIAPSPIEKLFSVN
                     PMLEQLCLVGRELPNNVLLVTLNEGVRLGMPKEEITHSLQETLKQVNSKLVKYEKVSH
                     ILVLKDAWTTENDKLTPTLKVKRRVVEKDYAELIQKALQQSQSIVWE"
     gene            complement(28851..32312)
                     /gene="mfd"
                     /locus_tag="I9760_000334"
     CDS             complement(28851..32312)
                     /gene="mfd"
                     /locus_tag="I9760_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAT5042129.1"
                     /translation="MNTKTLLYQPTKNKQIWGQLYGSSLALALAEYCQQTPGIKLLIT
                     QDNLCASQLQDELQFFLNPNQNTQELLFFPDWETLPYDQFSPHQDIISERLYTLSRVQ
                     QITNGIIISSASTLMHRLSPPEFLNQYALMLKEGQKLDLNSFRNQLQQAGYHCVNKVL
                     EHGEYALRGSIIDVYPMGSGLPFRIELFDDEIESLREFDPETQRTIEKINQIHLLPAR
                     EFPLNEQSQLHFRRAFRESFPGNPSHCPIYEAISNNQFPSGIEYYLPLFFDKTVTFFD
                     YLPANAKICLIEEIQENAERFWRELNERYEQRRHDVSRPILPPDNCFINPTELLTKAN
                     HYEQVRLYHKPSEKKGALNFNIAKAPQLPIDRKNIEPLRQLSEYCSDPTRRYLIVVES
                     AGRREVLLDLLKSSHIIPKTQNSWLDFIYDESPINITTGPLIYGCELTDKQIVIIVES
                     QLFGEQSTPQRRSTQKSVDPDLIIRDMAELRLGAPVVHLQFGVGRYQGLQYIDSNGTP
                     SEFLVLAYAGDDKIYVPVTSLHMISRYTGVDSEHAPLHKLGSEQWQKEKKKAAEKIHD
                     VAIELLDLYAKREAQPGHQYDFNQSEYIKFASGFPFTETPDQLQAIEQIIKDMQSPRP
                     MDRLICGDVGFGKTEVAMRAAFVAVQNGKQVCILAPTTLLAGQHFESFRDRFADFPVN
                     IELLSRFRSNKESEAVLAALQSGKVDIVIGTHKLFQNNISFKNLGLLIIDEEHRFGVK
                     QKEHIKSLRTHVDILSMTATPIPRTLNMAMAGIRDISLIATPPAKRLAIKTFWQEKND
                     LTIREAILREILRGGQVFYLHNNVQTIERVCQDLEALVPEAKIQSAHGQMRERQLERI
                     MSDFYHHRFNVLVCTTIIETGIDIPTANTIIIDRADKFGLAQLHQLRGRVGRSHHQAY
                     AYLLTPNEKLLTPDAVKRLEAIVSLEDLGAGFTLATHDLEIRGAGELLGEEQSGNMHA
                     IGFNLFMEMLDRAVNDLKAGKTPELSAPMHQGPEIDLRISAIIPEDYIGDIHNRLIMY
                     KRIANAKTTQQLRELQIELIDRFGLLPQPVKHLFLITELKLKAEQLGIQKISASAQQG
                     KLDFSEKPSIDPGTLISLIQVHAKRYQMEGPQRLRFTLDSTSAEERIFEISSLLNKLS
                     PHSQTID"
     gene            complement(32389..33651)
                     /gene="rmuC"
                     /locus_tag="I9760_000335"
     CDS             complement(32389..33651)
                     /gene="rmuC"
                     /locus_tag="I9760_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAT5042130.1"
                     /translation="MALFSSVTLFEGIALAIALQFLLLFWVLIKQAQQKKLTEQLGER
                     IQQALISQLHQLHIDLSQSYQTSQQFINEKIAQGQLATQQLISDAIQRQMADVREQMT
                     HSFKHHASSLTSHLQLLTEEIRNHLNTLTQQVNHKLTEGFEKTSSTFIDVVKRLTIID
                     EAQKKITELSSHVVSLQDVLVDKKARGAFGEVQLSTLISNMLPSASYQMQYTLSNQKR
                     ADCVLFLPEPTGNIVIDAKFPLETYQRLINSDALSTDRKSLQQQFRQDIQKHIKDIAE
                     KYIVPNETTDGAMMFIPAESIFAEIHANYPDLIAMSQKLKVWLVSPSTLMAVLTTARA
                     VLKDDATRKQVHIIQKHLQALANDFQRFEKRMDKLSKHIDLAHQDVNEVNTSAKKITS
                     RFQKIESVDIELDEHEVTQLELVESESI"
     gene            complement(33655..34833)
                     /locus_tag="I9760_000336"
     CDS             complement(33655..34833)
                     /locus_tag="I9760_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4785 family protein"
                     /protein_id="HAT5042131.1"
                     /translation="MKTAHLIWISAFCIAQAHAFTLPRQPTKAYECENCSQLSRENLH
                     DKWEITNEPLNNNISNVRRSYGYKERISLEQLQRGVIISTLAPGAVVRITPLQNKSIP
                     ELLIKTPKNQLLPLKEASSLYNQDDEVGNNPLAITKHQAMLQIKPELGYGKFILKSKD
                     ITNKYADAYMISVLDKFSITYLEVETDSLHYQYGDKLKATISLHNDITEYDVNDVDAR
                     LVGPKGQVISLNLTKLKSNVFEGTATLDSELNDRGENWYLETDVQTEYGQEIIRRSGH
                     TAFSYSIPSASLMNVKKLSSKPLTFVVTVDVATASRYALQSVLFQKNGKGEARPIQTS
                     QRAQWLEPGKHVLQFTFDNHNQLSDDNLYLGYLRLIDYGQLKTVYQYNQPVKLSQLVD
                     "
     gene            complement(34830..35729)
                     /locus_tag="I9760_000337"
     CDS             complement(34830..35729)
                     /locus_tag="I9760_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042132.1"
                     /translation="MELLRILSTLIVLLFSSLIHSHSDTNSNQGIAFVHGTRDHREDA
                     YGGYWKIDFIQSISQTLAKPENHYVVHCDFSQYMWHEDAANCVANQLLEFINDKNVSS
                     LTVYTHSDGANIMRWILSNPTYDGRYMTLKRKISQVIAIAPSSGGTPLADEVLNGGIF
                     ETSLAWLLGYLSDAVKQQRVGDMLIYNEELLLGSKGRPSLSIPFKVIVGTDVYASPFS
                     TSSYCNGYMLNSGLKIAKLYLNQCADGFLDCESQAQAGEVWFYDIDKTDYNLPLSHNQ
                     SRHSCFGLDQILISALVNEGVVK"
     gene            35952..37973
                     /gene="ligA"
                     /locus_tag="I9760_000338"
     CDS             35952..37973
                     /gene="ligA"
                     /locus_tag="I9760_000338"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAT5042133.1"
                     /translation="MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKAL
                     QQYEEQYPQFLSPDSPTQRVSGTPSDAFMPVAHKQPMLSLSNVFTTDELKAFIKRAIE
                     KLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPL
                     RLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEIS
                     ASRPLAIYCYGIGACEDYKLPNSHLEQLNLLKEFGFRVSPETRRAVGVEGCLDYYQYM
                     LAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAPRFACAHKFPATEEMTRLIAVDFQV
                     GRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEVVS
                     VILEKRPANARMIELPKNCPVCGSEVVREADEAIARCVGGLYCKAQLKRMMWHFASRK
                     AMYIEGLGSVLIDQLVDEGIVHHLADLYELDLQTLANLPRMGEKSAKNLLSALEKSKK
                     TTFNRFLYALGIREIGEAGARVLAEHYCDVESLKAATIEELMTLNDIGPVAASHIVHF
                     FAQAHNLEVIDRLLELGIHWPKPEKIQVNQQNPFFGKTVVLTGTLSTMGREEAKAKLL
                     ALGAKVSGSVSSKTDYVVAGSEAGSKLIKATELGVAIIEEDEFLKWVNS"
     gene            38075..>39441
                     /locus_tag="I9760_000339"
     CDS             38075..>39441
                     /locus_tag="I9760_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT5042134.1"
                     /translation="MRRYLLIQFMLFLMPCCVNLAYGSSWVFNNPYPESEANENIYYS
                     SFNEQPKTLDPARSYSSNEYLFIAQIYEPLLEYDYYKRPYELIPLTATQLPKIRYLDA
                     NGNPVPAGDNSKPAYSVYTIQIKKGILYQPHPALAQKKDGSYRYHHLPEDYLEEHDIG
                     KLSDFKYTGTRELIIDDYIYQIKRLANPAVSSPIYGLMSYYILGFKEYGDSLPKGLAN
                     KNSFIDLRKYPLKGVKKLDDYTFEITLKGQYTQFLFWLAMPFFAPVPWEADLFYSQKD
                     MDDKNLSFGWYPIGTGPFMLTENNPNRSMILEKNPNFREVYFPVNGSDADRRAGYLTN
                     LGRRLPLIDKAVYTLEKESIPRWNKFLQGYYDMSGISADSFDQAIHINRFGEPILSQE
                     MKDKKIYLRQTLEPSIYYTGFNMLDSVVGGNSKRARKLRQAISIAVNFDENIAIFFNG
                     RGIPA"
BASE COUNT        11614 a         6807 c         8591 g        12429 t
ORIGIN      
        1 ctaatttgtg ttatttactt taaatgcttc aaataaaagg tcttgtgtta ttctatcgag
       61 gctgttggag cagtaaatat catgaaatca actaagacgt gtatctaatt tgttgttata
      121 atagcatggc aattatgaca attcagtaga agaggtaagt aaaaaaaaat gagcatagtt
      181 aaagtacgaa atatttttct cattggtccg atgggtgctg gtaaaagtac tataggacgt
      241 gctttggcaa aggagctcaa actagaattc tatgactcag atgaagtaat tgaagagcgt
      301 gcgggcgcag atatatcgtg gatctttgat atagaaggtg aggagggatt taggcgccga
      361 gagcaaaagg tcattgatga attgactcaa aaaaccaata tagtattggc aacgggtggt
      421 ggagtcgtca ttacgcctga aaacaggaat gccctggcag gccgtggcac agtgatttat
      481 ctcaaaacat ctttgcaaca gcaatttgaa agaacaaagc gtgataccaa acgtccttta
      541 ttacaaacag aggatcttga gggacgcctg gagtcactca gagatgagcg agaacctttt
      601 tatgatgaat tggcggatgt cagttttgaa acagataaac tgactgtaaa agctgttgca
      661 aacaatataa taaaatactt atatggcgaa gtttgaactt tatgccgagg ttgatgtttc
      721 gatttcaggg catcaatatc caataattat ttgccgcaat ggcttaattg atcctgattt
      781 aattaatcgt tttatcactt caaaacaggt gcttattgtt actaatcgaa cagtggcacc
      841 tttgtatcta ggtcatttgc aatctgtttt accatcaaaa caatgcgatg ttgttattct
      901 ggaagacggt gaagagcata aaaatcagcg cagtttattt actatttatg attctttgat
      961 tcaaaacaaa catcatagag atacctcaat catcgctttg ggcgggggag tgattggtga
     1021 tatggctggt tttgcagctt ctacgtacca acgaggtgtg cgatttattc agctgcctac
     1081 tacgttactg gctcaggttg atgcttcagt tggtggtaaa acagcgatta atcatcctgc
     1141 cggcaaaaat atgattggca gtttttacca acctcaagcg gtcataattg atttaaatac
     1201 cttaaaaaca ttacctgagc gtgagtttcg tgcaggtatt gccgagatga taaaatacgc
     1261 tttgttggtc ggtggctctt tttttgaacg aattcaagac gttctgcaac aaggattaac
     1321 ggtacatagt ccggaattac cactattgat agcagagtgt tgtcaagtta aagcgaaaat
     1381 tgtagagcaa gatgaaaggg aatccgggtt gcgtgctttg cttaatctgg ggcatacttt
     1441 tgctcatgcg ctggaaacgt ataccgatta taaaaagtgg ttacatggag aagccgttgc
     1501 cataggttta tattgtgcgg ctgtattatc agagaagaag ggcttgctgg ataatctaat
     1561 tgtagaccag gtcgaaaaga tgctcataca tgctggtttg cctcataaaa ttccaaattc
     1621 catagattta attcaattaa gagagttgat gagtttggat aagaaaatta aaaataattg
     1681 tttacgtttc ataatgatta aaaaaccagg tgcttgttat attgatgatt cagttactga
     1741 agattgtttg cacaatacac tgattaatgt ggtagaagga gaacaaaaat gatagatgga
     1801 aaggagcctc ttgatgaggg agttgccaat cagcccagaa ctctattcaa accaggagcc
     1861 tggcttgcaa aagttgattt cattaatcat ttaattctct ttaataatgt actaataacc
     1921 attttgtccg agaaagaggg cggaaaaact tgttttaact ctttgttgaa aagccatttg
     1981 aaccaacaaa ttaaatcagt atcatttaag gcaaaaccgc catttgacag tcaagatatt
     2041 atcaatgagt ttgccaggca gttgcatctt aataataagg ataacaatat tggttctctt
     2101 gcagcacaaa ttaatgaaag aaaaacgcat gttttgctga ttattgatga tgcacatcac
     2161 ttgcctgaaa gttttatcaa ggaagtgatg attattataa aaaatcagga agattttgga
     2221 tttttccatc tttgtttaat ttctgattat tctatcgttg ccacattgaa taatcttgct
     2281 gttgagccgt ttaataattt aatacacaca atagaactcg gggcattaag cgagactgaa
     2341 accagaactt atgtattaca acgagcaatg gcttctcatt taatcaacag gccacttact
     2401 gacacgcaat ttaaacagtt ctatcactta accaaaggga acttatcaaa aatcaattca
     2461 aacctggaat tatttttatc caagtgcgct aatgaaaagc aaaataataa aaaggcaatt
     2521 ttaaaaagaa taagtttagt cgcgagcttc gctattgttg caggattgtc ctatttttat
     2581 tttgataatg tctataaagc acctgataat actctcaaac tgccagattt aacttctgta
     2641 acaacacttc cacccataaa tgaccttata aaaactccca cacaacaagc tgtccctaaa
     2701 caagaatctg taagctatat tgcttcatgg gaagattctt caacgaggca gctagtgcat
     2761 tatgggttac ccaaaaaaca aattttagat gattttatgg atgaagagga tagtcttaat
     2821 acagtagctg ttgtagataa ggtcgttgtc attccaaaac tgaaagctaa aaacatttca
     2881 gaacaggttg ggcaagcggt agttagtcag tcaaaattac ctcctattcc ccattcatca
     2941 caaacaaaat tagccagtga gcaaccaggc atcaagaaaa ttaatagtaa gctgtttact
     3001 atacagttgg ctgccagtca taaagtggag gatatcaaca ggtttaagaa ccaaaacaaa
     3061 tggttagctc aggcaaaaat aagacatttt actaatgcga aaggtagttg gtacatatta
     3121 actatcggtg aatatgagag caggaatcaa gctctgctga atataaaaaa actaccaggc
     3181 gaattagcca aactaagtcc atggatacga tctgtggagg gactttcaaa cattggttag
     3241 actaagataa tcagcttgga tatagctgtt agttgttgat tgattctggg aaaataggct
     3301 taaatctgct aatcttaact tagctatcag aattttaagg agaaaaagat gtataaaagg
     3361 gtattgtttg ctactgactt tgatgaggtt ggggtgcgag cagctgagaa agctaaaaaa
     3421 attgctgacg agaatggtgc tgatttaatt ttagttcatg ttgtagagcc tattccagct
     3481 tatgcttacc caggttttgc tggatttgca gaagtagaaa tgtctattcg tgagcaagct
     3541 gaaaaagagc tgaatgaatt ggcagaaaaa ctgggtgtag atgaaaagca tcgttttata
     3601 gagtatggtt caactaaaaa tgaaatccta agggtagcaa aagaacataa aattgattta
     3661 attgtgacag gaagtcatgg aaaacatggc ctttctttat tattaggttc aaccgctaat
     3721 gcaatcttgc acggtgctga atgcgacgtc ttgattgtaa gacccaaaat aagccactaa
     3781 gagcgccctc taacttaaga aattccgtac tacctggtag tacggataat gttttataga
     3841 tttctcaata tctcagaaaa ccagtatact atgtgttttt tattcttctg attaaaacga
     3901 ttcatgaaat tgttacgcag ttctactcat tttgctatat ttgataaggg tgcagttgcc
     3961 actattggca attttgatgg cgtgcattta ggccatcagc atttgattaa ggccctaaga
     4021 gcaaaggctg atgaaatgaa cttgccttta gttattttat tgtttgagcc tcagcctaaa
     4081 gagtattttc acagagagaa agcaccagca cgactttcta ccttgagaga aaaaatagat
     4141 gtcttaaact tatgtcaagt ggattacatc tattgcatca agtttgatgc aaggctggct
     4201 caaactcccg ctttgtattt tgctcagttt tatttatttg aagcattgaa aatacgctac
     4261 cttctggttg gccaggattt tcgttttggt aaaaacagag aaggtgatgt taatttactc
     4321 aaaacactag gtgctaatta ttcctgtgaa gtgacagtgc aatctgattt tcttatagaa
     4381 aatgaaaaaa tcagttcgac gagaatcaga gaagcgttgc aacaggggaa tttgcgtttc
     4441 gcagccaagt tgttgggaag accatacagc atgtgtggac gtgttatcaa aggagatggt
     4501 cgtggtcgcc aatgggggat accgacagcc aatttaggca ttcatcgttt atcgatccct
     4561 ttgcaaggtg tatatgctgt acaagtccat attaattcga atgtctttta tggggttgca
     4621 aatattggaa aacggccaac agtagctgga actaaaaata ttttggaggt ccatttattt
     4681 gattttcacg ggtctctata cggtcagtta gtgcaggtaa ttttcttgca caaacttcga
     4741 gatgaagtta aattctcaag tgtggaagcg ttaatcacgc agattaatag tgacatcata
     4801 ttggcaaaga cctatttaaa acagaattgg cttgatagtg cgattgattc atcgaatgat
     4861 gttgtgaatg gaatcagtaa taccgtttaa tttaccctta atcggggtat tttttgtaag
     4921 ctcccttcgg gctgtggaga tgaaacagaa caagggcatt taaaattatc aaaaacttaa
     4981 tgatttggca gagtaggcac ctatggcaga atataaagat actttaaacc ttccaaacac
     5041 ttcatttccg atgaaagcaa gtttgtcagt gcgagaacct gaaatgttgg cagattggca
     5101 agcaaaggga atttataaga aaattcgtaa agctcgtgat ggaagtaagc gctttatttt
     5161 gcatgatggt ccgccctatg ccaacggaca tctccactgt ggacatgcgc tcaataaaat
     5221 tctcaaagat attattatta aatcaaaaac ctttagtggc tttgatgccc cctttgtccc
     5281 cggttgggat tgtcatggct tacctataga attgaatgta gaaaaaaaag taggtaaagc
     5341 agggagcaag attagtccaa gagaatttcg tgccaaatgt cgagaatacg cagcaagtca
     5401 aattgatata caaagagacg agtttcaacg attgggagtc ttgggagact ggtataaccc
     5461 ttatgtgact atggattatc attatgaagc caatatagta cgagctttag gtttaatgat
     5521 taaaaatggt catttacaac aaggctttaa acccgttcat tggtgtatag attgcggatc
     5581 tgcgttggct gaagcagagg tggattatga ggataaaact tctccatcca ttgatgtggc
     5641 gttctcagca gtaaatccat ccgagttcct taattgtttt gaaactcagc cagcggtgaa
     5701 accattaatt ctgcctattt ggactacaac gccatggaca ttgcctgcta acgaagcggt
     5761 atgtttgcat cctgaaattg attatgcatt gattgatgca ggtaattctt attatatcgt
     5821 ggctactgat ttggttgagt cggttatggc gcgttatgga atcagccatt acaaaacatc
     5881 gggctccgct aaggggcgag tctttgagca ttttaaactg cagcacccat tttataagcg
     5941 tcaggttccc gtagttcttg ctgagcatgt gacaacagag tctggtacgg gttgtgtgca
     6001 tactgcacct gctcatggcc ctgatgacta tttggtaggt cagttctatc agttgccatt
     6061 aataaatcct gtcatggcca atggatgttt tgctgagaat gtagagttat ttgcaggcat
     6121 ttctgtattg aaagcgaatg agactattct agcagttttg tcggagcgga atgttttgtt
     6181 agctagtgaa tctatcaggc acagttatcc gcattgttgg cgtcataaat cgccaatgat
     6241 atttctggct acgccccagt ggtttatctc aatggataaa tccaatttgc gacaagcaat
     6301 aattaacgag atagataagg ttaactgggt tcctgactgg ggtaaggcgc gcatcagtaa
     6361 tatggttgaa aataggcctg actggtgtat atcaaggcag agatcttggg gaacacccat
     6421 gccattgttt gtgcataaaa ccaccagaga attacatcct gacactttgg agttaataga
     6481 gagggttgct atgatgattg agaaatcagg catagacgcc tggtttgatc tggatagctc
     6541 cgagttgctt ggcgatgatg ccaaacacta tgataaaatc acagatacca tggacgtttg
     6601 gctagattcc ggaatatcac attatagcgt tttaaaacat aataatgatt tagattttcc
     6661 ggctgatgtc tattttgaag gatccgacca gcacagaggt tggtttaatt cctcattaac
     6721 aactgcagtt gcaatgtatg gggttgctcc ctacaaaacc gtattgactc atgggtatac
     6781 tgttgatgcc gagggaaaaa aactatcaaa gtctaaagga aattatgttg ctttagataa
     6841 attagtgaat cagcatggtg ccgatatttt aagattatgg gtagcgtcaa ctgattatcg
     6901 tcatgaagta agtatttctg aggaaatcat taagcgtaac gcggatgctt atcgaagaat
     6961 tcgtaataca gcgcgatttt tattagctaa tttatttgac tttaatccag ccagtgattg
     7021 tattgaggcc aaggagttat tagaactgga caggtgggct ctaaagcgtt gccaactttt
     7081 acaagaagaa ataattactg cctatgaaaa ttaccacttc catttgattt accagaaaat
     7141 ccataacttc tgcgcagtgg atatgggtag tttttatttg gatttgataa aggacaggca
     7201 atacaccacg gcaaaagaca gtatagcgag gcgatcttgc caaacagcga tgtatcatat
     7261 ggtcaaagca ttcactatct ggttggcgcc tatcttatct tttacagctg aggaaatatg
     7321 gcaaacaata cctgggaata atagcgaatc tatttttatt gagcattggt ataatgcctg
     7381 gccaactatt gacgcagtga acatggagga ttgggaacag ctgcacatcg ttcgtgatga
     7441 agtaaacaaa gctttagaag aaaccaggca aagaggtgaa attggttctg cattggcggc
     7501 tgaggtaact gtttatgctg atgccaaggt attaccaaaa ttaacgcgtt taggcgaaga
     7561 gttacgcttt ttattcatta catcagaagc gaaagcttgc ccaattagtc aatcccccaa
     7621 aggattagct gttactgatt gtggggtatc aatacaggtt acagccagcg ttcacgaaaa
     7681 atgtgctcga tgttggcata gacgggaaga tgttggtcaa aatcaagagc atcctgaact
     7741 gtgcttgcgc tgtgtaggaa atatcagcgg atatcatgaa gagaggcttt acatatgaaa
     7801 aaatggcctt ggcttgtact tagtttttta gtaattattt gcgatcagtt aagtaagtat
     7861 tgggttgggg tgttattaac tccatataaa cccatgccag ttatgcctat gttgaatttc
     7921 actttagctt ttaacacagg cgctgctttt agttttttaa gcggtgcagg cgattggcat
     7981 aggtggtttt ttgcagggtt tagtttttta atgagtatta ttttgctgat ctggttagta
     8041 cgtactccag aacaggcacg atttcaatct gttgggataa gtcttattct aggaggcgct
     8101 attggcaact tgattgacag aggtcttcat ggttatgtga ttgattttat tgacgtttat
     8161 tataaacacc accattttgc cacgttcaac ttggccgata gcgctatatg tataggagct
     8221 gcaattttag ttctggattt attgattcgt agggaataga ctcaaactgc ctagaaaata
     8281 atgtcataag ttagtatcct catgtttgac tatcccctaa cagtagggtt tgcgttctgg
     8341 ttagatgcaa ataatactga aaaagcacag aatagatggc tgtttttgtt caagatgggc
     8401 gaaaaaagac tttctcggca ggaggctgat ctcttacgga aataatagct ccgaagtggt
     8461 ttagtatctt aacccttttc aaagtcaaaa tactatacca aagcttgtca tgcattaatg
     8521 gaaaacaatg cttaccaatt agaatcgatt tattttcaga taattgattg tgctaacctg
     8581 gctaatccat tcagttgctt atggtcaggg ttatgaataa ttactgctgc tacagctggc
     8641 aagtttagga aaagctcagc ttcattccag tcaatttcag ctgggaacag atttaatcct
     8701 ggtaaatatt tgcttagagc attacaaaat gtcagattgg cggggtaggg tcgcacttgt
     8761 ttaaatccaa tattcgctgc ggccatagct tctgatatgg tagctacact tggaaaatag
     8821 cacatcgaat aaacacttgc agtttgggct atagaagcta aaaagccggg gctagaggca
     8881 aaatgtacac ccgcctggta gcattgctct aattgttgag tatcaattat gccgccggct
     8941 ccaattttta gagatgggta ttgggtttta atctttacca ataaattggt atcggacgag
     9001 ttgatctcta ctaaatccaa atcggaattt gcaatttgat cgagtctgtc aaataaaaaa
     9061 ttatcgatat ccaatgatat gatcacagat tgattaccta agattttttc aataatcatg
     9121 ttttattccg aaaattctgc aataatcttt caatgttaga agacttgctg attgttggca
     9181 agggtattga taagaaaaac aacaatttgt ttgatgctga ggtgggggta gagcaccctg
     9241 aattatgatt ctcagataat aatccagggt agggagatga aagataattc tatcttatga
     9301 tgtcttgaat ggagataaag acggagtagg gttaaatcgt tgctcaagga cttcatctgt
     9361 tttgcttctt atagaagtaa cacttggttt tgtagtatct accccatatt gctcaagagt
     9421 ttgcagcata cgttgcatag tttgaaacgg tattgctcca gagcgcggta cttctttaag
     9481 ttgttcagca aacttaagca cttcattctt aaaataagta cgtgctttgg gatcggcaat
     9541 tttaatactg agggccataa tatcatcaag ggtgacttta acatcttcat tttttttcat
     9601 ttcatctatc ttcgtcttga aaaaagtagt cactgacgcc aagcttgggg ttggtttcgg
     9661 tgaaacagtt gtacttcctc cggaaggtgt taagacaggt gattcagtat ctaaagttgg
     9721 ttggttgagt tgatttaaag ccacaccaac tgtagattga agtttactta tgttattggc
     9781 tatttctatt ttttcttttc cattgtcttc taattgttgt ttcgtctctt caattcgttc
     9841 tttatgaatg gttgtttcta aacctttatt atgatgtact aatttcttga ctgtcattgg
     9901 tgtttcatac tgaagtttat gaaaatcatg ctcagctttt ttttgtttct ccgggctatc
     9961 tttgatagat tcccagtctt caccttgttt taaaagataa aatttgccgt ctttttcaag
    10021 tattttttgt tctttatgga taggatagaa tttaccgcct aaattaaaca gttgttgatc
    10081 ttttcctatc acgtaatgcg catcatttaa tgatgttact ttctctcctt tctcattaaa
    10141 aaaggatttt tcttttctat gtactgcaag catgtcttgt agatttgcca attttaaatt
    10201 taatccatta tgtttatgca gcaatttggt ggcttcctca ttttcttttg gatctgtgct
    10261 cataataaga tcatttattg tttccaattg tttttccatt tccaaactaa gcttatgaat
    10321 tagagcttcc atcttgatgg ttgcaagatc atgtttagat tggctatagt ctttttgggc
    10381 tttttctaat gctgcgggat catttttaat ggcatcccat tggccttttt gcaggagata
    10441 gaattgtcct ttatgttcta ctacttcctg gtctttatta atagccaggt gcgcgtcttt
    10501 taatgagctg accgggtttc ctttttcgtt aacataggat tttttacctg tatgagcgtc
    10561 atgtaaaact tgcaggttag ccagttttgc ttttatgtca tgatgctcac gctgtaagga
    10621 ttgaacggtt ttctcatcgc ctttatgctt ttccatcagt tgcgtgagtt tctcactgtc
    10681 tttgctgaag gctttcattt tttcattaat ttcggatagt gataactctt cagagtcagt
    10741 gagaagtttt tcaaatcctt ccaaattttc ttcataggtt ttgtacttgt cttcaagatc
    10801 tttgccttga cgttccagct tatccagttt tttttctaat tcttcgcctt tcttaatatt
    10861 ttcttgagca cgtttaatag cttgttcata ttcatccagc attttttgaa tttctttatc
    10921 agcttgtgaa gtggatgaag tggactgttg gcttggcttg cttgcttttt cagcattttt
    10981 tatggctttt tcattgtatt ctcgaataat ttccttgact ttttccgcag catgggattt
    11041 tttggataaa taccacaaaa acaaagcggc cattaatcgg cgtttaaaga gctcatgctc
    11101 cattctgtct tgttgatttt gtagattgat gtttctttgc agagccattt gggcacctaa
    11161 ctcagctatt acgtctttac ccgccggaga taaaagaaat tttttgacct gggatgcgtt
    11221 ttttaaccct tctcggccca gattaatggt ggaacctttg tcttccttag cctggggatc
    11281 aagcaagctg gattctatcc atttctcaaa agaagtttta aagggcttgg ctggctcatt
    11341 gggttgagat tcttttttag tggtgggcat tgatagctta tcttcttttt tactatcctc
    11401 aacacgcatt ttttctacaa caggttcctt ttccttggtt tttacggatt caagactccc
    11461 ttctgatgtt ttaacttgat gtgatttgtt atcttttgcc ataaatagga tccttaatca
    11521 atcgccccca gttatcttat attatacaca atgttgacca ataaagcatc agtttattca
    11581 tgttttaaca ggattatgcc atttactatc gtgcgatctt gagtttgcaa ctcatgcatg
    11641 attggagtct ttttttgctg aatattagta tttcatctta gaagcaagta gcataatttg
    11701 caaataaaaa ccatgaaatc aatgcaatta gatttggaag tgtttagata gtcagtgtat
    11761 gttcttgagg cattgtgtct gatgtttgtg tggcaccatg aaacagacct ggaaaatgca
    11821 gttgttggga tttctgatta tctttgactt tattggtgga gctcttttcg tccaataata
    11881 aattacgtaa caggcagtga ctgtctgagt gaggaattaa ttcgcaacgt ctgggatcat
    11941 tgaaatagta ttgattatcc tgcactattt tatgtgtcag tatatcctca tcatggaatg
    12001 cccattgata tacttttcca cgatttattt ctattaagga aagcaaatat tgctttaatt
    12061 cacccgcttt gctgtctatg tggttacata atatagtcac cccatacgcg cttgttgaga
    12121 acaaaactcc tgctgaatga tcccaaatct gtccgaagtg ataaaggata gtaccggtag
    12181 aataggcaag aagtcgggaa ataggattaa taatagataa atttactctt attgttagtg
    12241 aaagtatgtc tatcagaggg agaatcaaaa tatttttaat cagctgaagg aggggtaaaa
    12301 aaagtagcct tatgacatta atgcctatgt ttgtgactgg attatctgaa aaatggccaa
    12361 agggggaagt aaatccataa agaggggaaa gcgtaaattc agatatgaag ttatttgttg
    12421 atggacgctc tgctatatcg atgagtaatt tcccaatcac actgaagtta cctaatacag
    12481 acagtaattt gcggaaaaaa ttggtaattc ctctaaaagg gacgtgtatt aaaagcgaac
    12541 tagcctcaag taaggggatg gttaaaatag ctaaaataag atcaaaactt gcggctaaag
    12601 tttgtttacc cagttgtttt atcaatttgc cacttttttt ccagccattt ataaatccat
    12661 caaaaaaacc atagtaaaaa ccttcaaaaa aaggtgcaac caataatttt ccaatattta
    12721 taatattctt gcaaaaccac ttgcaagtcc caaggaacca gtcatcaccc gggtgcataa
    12781 tggtatcata gatagcggca cctcctttac ctccgagagc agcataattg gcataaggaa
    12841 agtcacccat ttcatgttgc agtgaaggaa tcgcttttgt taaaccataa ccaaggctaa
    12901 gcgctaaaga aacaataata gcaatttctg ctgggtcttc tttcaagaca gagacagcat
    12961 cgacaaagaa cttatctaaa ttaccgctca ctaccatacc ttgccagaga gttgcagaag
    13021 cagagatagc ttctgaaatc ttgccatgat tcatcaggcg agctaatttt tgaacaggtt
    13081 caattccata aatcaagcca cttaagtgca gcttttttaa aatgtcagtg agaacattgg
    13141 gtgcgagtac agcacctgcg ccatagacat aagctgccat ggcaagagtt ccaatcatgg
    13201 ggttacgctc gctggtgtct ataaaaaatc cagctaaatg gcgtactacg cccaaagctc
    13261 tgtgcaaagg attttcatag gtgtgcacag tagggggatg attcgctctt gcaatgacat
    13321 aggggctgtt gttgatctga tcactaatga actcttcaag agtttgtcca tttgcgagaa
    13381 gtccctgatt ttggagcgct tcatgcagag aggttctttt aatttttttg gattttggac
    13441 gctgatctct ttctgaaaga tgttgtttag gggcatgagt tgcatgcaca tgattaaaaa
    13501 attcttcatg caggtcatgt atgatgaatt ggctcatgct tttgtaaaaa cctttaaaaa
    13561 aatcaggtat acgggaaata aaagctttga aacggaggct gattctttca ggcaagtaaa
    13621 gatgcctttt gctgatttgg cttaaaatat ataaatcctc tccatccggc aaaggataaa
    13681 agagttgatc atgtataaaa tatttgagat gaatcagttt attttcgagt cttaacagaa
    13741 cagtattttt ttgaggcaca ataacccaat taccattagg taagtggctt agtaatcttt
    13801 tcgcctcttt taaactgttt acctctgtct gttgggctga gttttccact agatgattta
    13861 ccatcgcagc cagactatta taacctcttt gaggcagtcc tttgagtttg tagtaaggcg
    13921 ttattttggt aatagatact ccatcagagc gtttttgtat ttcagtttta tgacttggtt
    13981 ttgttgtgat tgccagataa ttaatccaat cgtgaccgtc atgccaaagt ttacttatga
    14041 atcgttccag ttttgcaaag cgattggccg ggatgttgtt taatgctggc gcagggcaga
    14101 gttggtattg ctggcaaatt accctgagcg catcgtcaag ctcactgcta ctgtgcccaa
    14161 tatggatttc actatcccaa acaaaacaag gagtaattgg ttttccaagt tgtgttttag
    14221 tcactttggt aaagtccatt tgatcctggc gagaaacaga aaaaactcgg ttaaaccgat
    14281 aatgctgtgc tgttttgtgc gcatcatata gcatataacc aaaacttcct tctgaaacca
    14341 caggattatt ggctatcatt cgattgtcgt tctggcaatg ttcaataatg gaaatgattt
    14401 ttcttcttaa ctcctcctgt ccagcatgat catgaggttt taattgtctt tcttttttca
    14461 atagatggtt taaggaacgg atgcaagcat tagccaggtt aattttttct tcctgctcat
    14521 ggcttagggg ttttttatag ctttcagctt gaactttata gcgattgatg tgatttataa
    14581 catcagcaac tagcgaagat ggtctgtctc ccggaagaat ttttaacagt tcagggttat
    14641 gagtgaatag catcagctga actgttttct tgttgtgatt ggctattaag cgtagtctgt
    14701 ctcctccagg aaggagatat tccatattat aattaccata aaatactgta ccggcttcgt
    14761 gactgaaaat acttccagtg atagttggca tcgctttgtg tgtgtgcgga gtaactctga
    14821 gtggaataat aacttgagtt tggccatcag tagtttcaaa atcaggcata aagatttatt
    14881 tgttttaata tatggtaaaa ggatattgtc attaatttag tgatttttta tcgcttaagt
    14941 tggttttgaa gaaaagtaaa gcccatttaa gctaaaataa tacaaaatcg tttaatttaa
    15001 tgccattaat gcacagggta ctcgaattgg aacagaaata gcaagcctat ttaatgaaaa
    15061 atttggtatc tctggaagtt ttatgacatg gctgttagaa tgttttaaaa ataaacagga
    15121 atcaagatta atgagtagtt attgtggtta tattgcttta gttggcaggc caaatgttgg
    15181 caaatcaacc ttattgaatc gtattttaca gcaaaaatta agtattacct cgagaaaacc
    15241 acaaaccacg cgccatagta ttttaggaat tcgcacagaa ggtgaatttc agtttgttta
    15301 tgtagatact cctggtattc atcagggtaa tgcgaaagca attaatcgca tgatgaataa
    15361 aactgcgatt agtgtactac gtgacgtgga tgtgattgcc tttctagttg atggaacgca
    15421 ttggaaagac gaagatgagt atgtgttgaa tttaatcaaa caagctaaag ttccttgcat
    15481 cctggtagtt aacaaagttg acaagataac tgataaaaca caacttttgc cctggataga
    15541 gcaaataagt cagcgatatc aatttgcagc cattattcct ttgtcggcga aaactggttt
    15601 gcaggtggat gagctggaag gcaaattaaa gaattatctg ccagagggac ctcatttgtt
    15661 tcctgatgat caattcaccg atcggtctac aaaattttta tgtgccgaat tgctccgtga
    15721 aaaaattttc cgcttttgtg gacaagaatt gccttattct gtaacagtag atattgaatc
    15781 atttaaggat gagggcaatt taattcgtat tcatgccttg attttagtag ataaggataa
    15841 tcacaagcgc atgatcattg gtgataaagg ccaaaaatta aaagaaatgg caactaatgc
    15901 ccgtttggac atggaaaaaa tgctagataa aaaggttttt ttacagtgtt ggtgtaaagt
    15961 aaagtcaggc tggtctgatg atgagcgaat actgaagcaa ttgggatatg accagtaaat
    16021 cgctgaatgc ttgggtaatt cataagcaat ggtctggaga taccagtgcc agactaaagc
    16081 tttttactag ggaactggga ttaattaatt gcttgtgcaa gggagggcga actcccaaga
    16141 aacagtcatt acttcaagct tttattcctc tgtgggttag tatcgaagag cgttatgatc
    16201 aatattacac acgaaacatt gaaagcactt catccagact ggatcttgag ggacattctc
    16261 ttttttcagg cctgtacatc aatgagttac tctattatac gctgagcccg gatttccccg
    16321 acccggattt atttgatgct tacttgttca ccttaaatgg catagcatta gctagagaaa
    16381 gagaggcgat agaagctttg ctgcgtcgtt ttgaatgggc tttgcttaag gcttgtggat
    16441 acacgttttc atttcttcat gaggcgcgaa cgggagagtt aattgttccc gattcttatt
    16501 accaatttgt agctggggag ggttttattt taggtggtga taaagaaata cctggtgagc
    16561 atttacttgc tatagcagca gataatttaa gtgagtccgc ttatttaaaa tcagcgaaat
    16621 tcattatgcg tcaggcaatc aatcatcttt tgggtggacg ggaaatcaag gctcggtcct
    16681 tgtatggacc ggcctgagat agtggtatga tcgccaagtg cttcgatgct atcaaatcag
    16741 gtctagggtc tattatgtga ggttaggtct ggatagattt tcgatttctt ccacaggatt
    16801 ttgctcctga attttaataa aaccaatttg ctcttttaaa gtacctttag gattgagttt
    16861 acttcccaat gaaatagcaa gattatctaa tctatcaacc aaagcacgtg aggtagtgta
    16921 attaccagca tcaatttcct tgacaatgtc atcaactact ttgacatggt gtctattcca
    16981 atgaaaagtg aaaattcgtc tgaatttggc ccaccaggtg ttgcctcctt tagtataatc
    17041 atcaagaaca gttctaatgg catgtatctt tttgtattct acatcatttc tgtggagctg
    17101 atggcgagga tcattcataa tttcttgaaa agcatttcgc cataattcat gatatgcagc
    17161 aaaatttccg gggatcgttg cagaaacagg ccttcttgta ccagggaact ggctttctgc
    17221 gcgtacttct tgtcttccag ttcgtgaagg tgtaccagta tagggagtga gctggcctgt
    17281 tctggttgaa gtaagactct cgtcaagagc tgactgataa ttcactgtgg cgggaaaaag
    17341 gctgcgatta taagcaaatt gttctttggg aatcgccaat gcttgttcag gagggtgtcc
    17401 catgccaacg gcccagggat caacgattag cgtaccaatt ggaaaaactt cagcggttaa
    17461 ttgctgtggg ggttgaggga gtctgcttcc tttaggaacg agtaaggtaa tattatgtaa
    17521 ataccggccg ttactatgca tacttcctat aacggcaaca tcatactgag ttcttatttg
    17581 tggatcatct aacaatactc ctgcagagtg gtaagcaaaa ttgctgcatt cagagacacg
    17641 aactgcctcg ccatcaattt taaaacttga ttttttattt ctttgctgag caaatttaat
    17701 atattcctga gccgatttag gggttaagag ctggggagct ccccattgac taaaaggtgg
    17761 tgttctggat aaggagtttg tactgtattt ttggtatctt tgtccgctag ctatgccttc
    17821 aggagtctga gcaaggcaat gtggaaaatt cccaatatca gcaacaacaa atttttgata
    17881 ttgttcttgc ttcagtctct cttgtatctg ttggggggca atagccataa attactccga
    17941 taactgattt gagtttattg tggcggttat ttcttaacca tgatttcttt aaaaatatag
    18001 tgcttttctt tttatggata tgaataaaaa aattacctct tgggttgtta tttttactga
    18061 atcttgatta tgataattgt gatttcataa ttaattcggt tttttattta agcctttatc
    18121 gctttctgcg gtttcttgac aaggtgacat acaactgaga aaagtaaatt tttgcaaaaa
    18181 cttaaataca ctatgggagt ctggcgattt tttagaataa tttagttcat ctgtttcatg
    18241 cacttcaatt ggattgccga ttaaatcgag gtccgttatc ctggtattgg tttcaagtat
    18301 ttttttacct gctgctgtaa tttgattact acccagatga agggtactca aagacgtgtt
    18361 attagcaaaa tggagtgttg atttatcggt tatcgcgttt tttcccaaaa taagtaaagt
    18421 cagttgattg tttttagcta attcaatagc tccaatatca gagacatcac aagcgcttgc
    18481 aatcagctct tttatattga tattacttgc taatgctata accccctcat cttccagact
    18541 attgaagcag agatcgattt tcttaagatt ggattgagca agatagcctg ctcctacttt
    18601 ggttatctta ttataaagtg cataaagggt tatcagtttg gcgttagcgg ctagagcttt
    18661 aactccctcg tcaccaatct cgttaaaact gacatctaaa gtagtaatgt ggttatcttc
    18721 tgatagccat tgagcaccct ccggaccaat attattatgg ctaatgttaa gggatgaaac
    18781 cgatgtttta ttaacaaaaa gcttcacacc attggctgta atatcattat gtgacaggtc
    18841 aagactggtt atggcaggat gattttgaag aaactcacat agcagtggta tgtcattatc
    18901 tctaagattt aaaaaggata atgagagttg attaagattt ttatcatagc ctttttccaa
    18961 atccgataat gttaacacca aacaccatgt tcaattataa ttaaaattgt ttaatatttt
    19021 acttgaacaa gaaaatcata tcaagttttt tgttcagttt atgatgattc agggaaatgt
    19081 tatcagtgcc tattattcag gcatcagttt gacttcaata ttataatttc tttaaagtgg
    19141 tagtgaataa agtaaagtat ggttaaatgc tttaggtagt gccgtttaaa ctgattgaaa
    19201 taaggtaacg tgaatagcag tttgtcaaga actgcattat actctgcatt ttatattttt
    19261 tggtgcaaaa tgaataaaga gttattgtta ggtgttaata ttgatcatat agcaaccata
    19321 agacaagcac gaggaactcg ttatcctgat cctgtccagg cagcaatgga tgctgaagaa
    19381 gcaggggcag atggaattac tttgcatatg agagaggact tacgacatat tcaggcgcgt
    19441 gatgtcagat tgattaaaca ggttctgcaa acacgtatga atcttgagct ggcggttact
    19501 gaagccatgc ttgattttgc cgaggaaata tcgccggagc atgcttgctt agtgcctgaa
    19561 aagcgggaag aactaactac agaaggtgga ttggatattt tgactcaccg aaatgtggtg
    19621 gaaaaggcag tacgtcgctt acaattaatg ggaagtgagg tttctttatt tattgacccg
    19681 gataaagagc aaataagagc tgcggttgat gttggggcgc cagttatcga attgcacact
    19741 gggtgttatg ctgatgccac ctcagaggaa aagcagcatt atgaattgca acgaattaaa
    19801 gaggcagctg aatttgctgc cagtttaaac ttggtagtga atgccggtca tgggttgcac
    19861 tatcataatg ttaaaccgat tgcagcgatt agggagttaa atgaacttaa cataggacat
    19921 gccattattg ccagagcatt attctgtgga ttaaaagagg cagtaagaca catgcgccaa
    19981 ttaatgcagg aagcgagact ttatgtcaac gactgatgtc attgttgtac gtatcactgg
    20041 cgattctggt gacggagtac aattggtagg tgaacaatta accattagcg cggcattgac
    20101 aggacgtgat gtgcgtacta tgcctgattt cccggcagaa atccgggctc ctgctggaac
    20161 agttgcgggt gtctcaggat ttcagttggc aatggcggaa agggctattt ttactgccgg
    20221 agagtcattg gatgttttag tggctttaaa tccagctgca ttaaaaaact cacttcaaca
    20281 tttgaatcaa ggtggtttat tgatcattaa tgaagacagt tttcaagaaa gagattggca
    20341 aaaagcaggt atcgataaga gtttcctgga taattgtcga gaacattatc acattgttgc
    20401 tttcccatta attacacaaa ctattcaggc agttgaatcc attaacctta caaaaccaca
    20461 ggctgtcaaa accaagaatt tttatatttt gggattggtt ttgtggttgt ttgatttgcc
    20521 tacagaaact tgccaggctt ttatcgctaa aaagtttaaa acaaatcagg ccatagcaaa
    20581 agccaatgaa ttggctttgt tggcaggata taattatgct atgactctgg agctttcccg
    20641 tcgggattac atgctggggg aagtcaacag gcattcaggt gagtacaggc aaatcactgg
    20701 agttgaggca gtagggttag ctctggctac attagctact cagacacaag ttccactgct
    20761 ggtgtcaggt tacccaatta caccggcttc tgctattttg catgaatgtg ctcgcttatc
    20821 tgattacggc gtacagttaa tacaagccga ggatgaaatt gccgctattt gttcttgtat
    20881 aggggctgct tatggtggtc gtttagctct gacctgtact tcagggcctg ggcttgattt
    20941 aaaatgtgaa agcctgggct tggctgttgt gacggagctg cccttggtgc tagttgatgt
    21001 gcaaagagcg ggggcttcaa caggtttgcc cacaaaaacc agccagagtg atttaaagca
    21061 agctttgtat ggtcgccatg gcgaggcacc tttacctgtg attgcagctc aatcccctgc
    21121 tgattgtttt aatacaatta ttgaagcctt caggatagcc atcaagtaca tgactccagt
    21181 tattgtatta ctcgatgctt atttggcgaa cgcggcagag ccctgggaaa ttcctgcttt
    21241 ggattctctc aatataccaa aaccggagtt taatcgtttt accaaaccct atcatcgaga
    21301 tgaattttta agtagaagct ggaatgttcc tggaacttcc gggtttattc atcaaattgg
    21361 aggcttggaa aagcaaggcg atgaaggacg agttagttac gatgctgaga atcatcaaaa
    21421 aatggtgatt acacgcgccg agaaaataaa gggtattgca agagattatc ctgctttggt
    21481 tcttgagggt gatatgacgg ctcccacgct tgttataggt tggggtagta cttttggtag
    21541 tttaaagtct gctgtacaac aatgtcttga acagggatta ccggttgcct atctgcatct
    21601 gcgccatctt aacccattac ctgatgattt gggctctttg ttgaaaaaat atgacaaggt
    21661 attggttgct gaattaaatt ctggccaatt atgtcaactc atcagagcag tttatctggt
    21721 ggatgcgcaa tcgattagcc aatgcaatgg tcaacctttc agtgttaatc ctttggtaag
    21781 tgccattaaa gcggaagcta actatgaaca gcaattataa gcgtgaagat tttgtgaatg
    21841 aaacagaagt tcgctggtgt cctggatgtg gtgattatgc cattttggct gctttgcaaa
    21901 gagtacttcc tgagttaggt ttgcctcctg agcaacatgt ttttgtctca ggcatagggt
    21961 gtgctggacg gcttccttat tatatgaaca cttatggatt tcatactatc catggcaggg
    22021 ctacagcagt agcaactggt ttaaaggcaa tgcgagaaga tttatgtgtc tgggttatca
    22081 ccggagatgg ggatgcgtta agtataggcg gcaatcactt gctgcatatt ttaaggcgta
    22141 atgtcaatgt taatatactt ctgtttaata atcaagttta cggattaacc aaggggcaat
    22201 tttctccaac gtctcagaaa gggcaggtaa ctaaaacatc ccccaaagga gtgacaagtg
    22261 agccaatcaa tccaatcatg atcgctttgg cttccggagc cagttttgta gccagggcgg
    22321 ttgataaaga tcctgtccat ttggcttctg tgcttaaaaa agcttatgaa cataagggtt
    22381 gctcttttgt tgaaatatac caagattgca atatttttaa tcatggtgct tttgatgatt
    22441 ttgcagttaa aaataaccgg gctgaaaaca caataatatt ggaagacggt cagccactcg
    22501 tatttggcgc tcaaaaagaa aaagcgctgc agttggacga tgagcaattt gtacaaattt
    22561 caattgatga aaaaacgctt tataagcatg atgagaaaaa cttaattagc gccatgcgtt
    22621 tagcgcgatt gacgttccct gattaccccg tacctttggg agtatattat caaaaggatc
    22681 gtgagcgttt tactctgagc caggaagtga agaaaagacc ggaagatttg ccagaacttt
    22741 ttaaagccaa ggctagttgg aaacaaaatt aatttcacat cgattgatca tacttctgga
    22801 ttttataatg taattattag taactgtcca gggtatgaga atttttctgc ttataagcct
    22861 atttatttga gttgtctttc ccgcgaagga gggaaaggca taggacttaa tattccttaa
    22921 ttacatagcc gtgaagggtt gacaattaaa aggcgcaggt cgtctcagta tagataaatt
    22981 gccttgtacc agaccagcgc ctccatgttg aaatattctc ttacatttcc ttttaaacaa
    23041 ttgatcgata aaaaaatccc ggcatacagc cgggactcag gtagaggaga cgatattcaa
    23101 ttagtcgcga cctgagaatg cgcttaacag gttcagcagg ctaacaaata agttataaat
    23161 ggaaacaaac aaaccaattg tcgccattat gtaattggtt tctccttcat gaattatggc
    23221 gctggtttgt aatagaatca atccagatga aattaataca aaagcagcag aaattactaa
    23281 ttgcaaagct ggtatttgta taaatattcc cgctatcatc gccaataagg caaccataac
    23341 gccaacaaaa agaaatccac ccaggtagct aaaatctttt ttcgttgtca gggcatatcc
    23401 tgatagagca aaaaagatca ttccagtacc acctaaagca gtagcgacta tttgtgggcc
    23461 atttgaaaag cctgtgatgt aatagttgag taatgggcca atggtatatc ctaaaaaccc
    23521 tgtaaaagca aagacgctta ccaaacccca aacgctattt cttaaagctt gagttagaaa
    23581 catcaaacca taaacgccaa ctatcattaa tagtggattc attgggcgag ctccactaat
    23641 aaaggctacg taggcagtta atgcgctaaa tatgaaggtt aaacttagca gtaaatacgt
    23701 atttcgcagg accttattgg ttgcgagcac agattctctt tgttgactaa gtatggtgat
    23761 atcatttcgg ttcattgttc agaactccag ttatttatta tcagtgtagc atatctatgg
    23821 tcaaatttcc tatttttcaa gtcgcatcac ttgaaaattt tgtaataaaa tttacatata
    23881 gaccaataga caatttatta ttaaggcgaa aaggtttttg tgtcaatttt gttttgggct
    23941 tatatggaac gatatagtaa gatgaaaaat atgaatgtca tttaggaaaa aggatgaatc
    24001 gagtagcgct tgtgcaaatg gtatcatctg ccaaaatagc agataatttg caactcactg
    24061 aacaatattt aattgaagcc agggcacagg aagccagttt ggttgttcta cctgagaatt
    24121 ttgcttttat gggaatgaat gaacgagaga agttgcagat tgccgaatat tatggtcaag
    24181 gacctataca acaaagaata agtgagttag cgagagattt gcgggtatgg ataatagcag
    24241 gaacaatccc gttaaaaagt atgggttcaa aggtaagagc aagttgtata gtttatgatg
    24301 ataaaggcct aaatgttgct cgttatgata aaattcattt atttgatgtc atagtttccg
    24361 agcaggaaaa acaccaggaa tcgttaacta tagaagcagg aaaagatata gcgcttgtgg
    24421 atacgccagt ggggaaaatt gggttaacca tctgttatga cttgcgtttt ccggaattgt
    24481 accaatattt aacccaaaga ggcgcacaat tactttcagt tccgtctgct tttactgcgg
    24541 taactggagc ggcgcattgg gaggtcttac tcagagcaag agcaatcgaa aatttatgtt
    24601 atgtcttagc tcctaatcag ggtgggactc atgaaaacgg gcgtcatact tatgggcata
    24661 gtatggtggt ggaaccatgg ggtaaagtgt tggcacaaaa agaagaaggg cagggagtaa
    24721 ttctggctga tattgattta gagagattgt cacagttgag aagacaattt ccgtgtgttg
    24781 aacatcatgt tttaatgtaa aaggtatata aatgacaaaa tctcttgcga tagcaaaaaa
    24841 tattttattg acaccagctt ccctggatga agtcggcata gaaaaattat tcaagaccat
    24901 gtttaatcgg aacgtcgatg atgctgatct ttatttccaa agttgtagtt atgaatcctg
    24961 gtatttggaa gattcagagg ttaaaagcgg caatttttct ctggataagg gggtagggat
    25021 tagggctgta agcggtgata aaaccggctt tgcttattgt gatgatatat tgctgccttc
    25081 catgcttcgt gctgccgatg cggcgcgctc catagccctt tctggttcaa aaacagtacc
    25141 ggccgtcaag gtattaaagg cctctgtaaa tcgatatgaa agtttaaatc ctttggaagg
    25201 aatgagcaaa caggagaaaa ttgctttatt ggaagccatt gataaagagg cccgtagtct
    25261 tgatcctcga gtgatacagg ttaatgccac tttaactggt tgctatgaag tagtaatggt
    25321 tgccaatatg gatgggcaga tgatggctga tgtgaggcca ttggttagca ttaatgtcag
    25381 tgttattgtt gaagacagtc atggtagaag agaatcggcg agatcgggtg gtggtggacg
    25441 agtagcttat tcctatttta ccgagaaaga aaacgccttg agttatgcgc gtgaagcggt
    25501 acgggaagca ttaaccaatt tgaaggctga gccagctcct gctggaacca tgccagttgt
    25561 tttgggccca ggatggccag gggtacttct tcatgaagcc gttggacatg gtttggaggg
    25621 tgattttaat cgcaagggat tatctgcttt ttcaggcaga ttgggtcagc aggttgctgc
    25681 tgcaggcgtt acagttgtgg acgatggtac tttagagaat cgacgtggtt ctctgactat
    25741 agacgacgaa gggacgccat cgcaatgtac gaccttaatt gataatggaa ttttagtgaa
    25801 ttacatgcag gacaagttga atgccaaact aatgggaatg caatcaacag gtaattgtcg
    25861 tcgtgaatcc tatgcccatg tcccaatgcc gagaatgacc aatacttata tgctgtcagg
    25921 gcagtatgac cctcaggaaa ttattcgcac tgtaaaaaaa gggttgtatg cggtgaattt
    25981 tggaggcgga caagttgata ttacttctgg ccagtttgta ttttctgcga gtgaggctta
    26041 tttaatagaa aatggcaaga ttacaaaacc ggtgaaagga gctactttga ttggcaatgg
    26101 ccctgatgtg atgaaaaaaa taagtatgat tggtaatgat ttggcattgg atagagggat
    26161 tggtgtatgt ggaaaagaag gacaatctgt tcccgttggc gtagggcagc ccactttgaa
    26221 aattgatgcc ttgacggttg gcggaaccaa gtaagaaaat gccagtcaag agtttaccaa
    26281 ctatttccag gagttattct tgacaaatca ataccctaaa attggtttgg ctttaggtag
    26341 cggatcagcg cgcggctggg ctcatatcgg ggtaattcaa tcaatcaaac gattgggtgt
    26401 aaggattgat tgtgtcgctg gatgttccat aggcgcattg gttggcgcca tttatgcctg
    26461 tggcaccctg gatctgtttg aacaatgggt gttaaagctt aataaaagag aaatggctaa
    26521 actcattgat gtgaattttt taggtggcgg ggttctctct ggtagtaaat tgatgacctt
    26581 tttcaaggat tttggtcttg aaaaaaatat tgaagagctt tctctacctt ttagttcagt
    26641 tgctactgat ttgagtactg gacgtgagat atggctaagt aaagggtctc ttgagcttgc
    26701 tattcgctca tccatgtctt taccagggtt atttaccccg cagcgttata aggattgctg
    26761 gttagtcgat ggtggtttgg ttaatcccgt tcctattaca ttgtgtcgag ctatgggagc
    26821 agaggttgta attgctgtca atttaaatca tgacttgtta agagtacctc cgagcacgat
    26881 agaacacccg gggttttttt cagttctctc aggccaattt gataaaatga taaaaagaat
    26941 aatgggtatc agtgatggac ctggatatta tgatgtcttg accagaagca tcaaaataat
    27001 gcaggaccgg ataacccggg caagagcggc aggtgatcct gctgatttga ctttggttcc
    27061 tcaattagga aacatcatgc tttatgattt tcataaagca aaagaggcga ttcatgaggg
    27121 tgaaaattgt gttttgcgag aaaaaaataa tattttacaa ctagttggtc tagaatagta
    27181 attaaacaag cttaaggaag agctatgcca aaagaatcca attccaattc attatttgac
    27241 ttactgttga aaaatgaaaa aaattcggca gaccgtgtgt atttaagaca gccaaaaaat
    27301 ggagtttggc atgaatacac ctggtcgtca gtgatgctgc aggccagaaa agtagctggt
    27361 tttttacacc atctggggtt aaaaaaaggc gatcatattt ccattatctc aaagaattgc
    27421 gcggaatggt ttatcacaga ttttggaatt catattgctg gcatggtcaa tgttccatta
    27481 tttgccaatc aaaatgagga aagcgcgcat tatgttttag atcatgggga tgtgaaacta
    27541 gttttcgttg gaaaattaga tgatcatcaa cgagtcaggc aatttatccc taagaattat
    27601 cgcacaattg gttttgatta ccataaagat ttgcaagttg attacgaatg gtctgatgtt
    27661 ctgaaaagtg accctatgat taacctggtg gagccagagc cagatgcgtt gtataccatt
    27721 atttattcat ccgggacttc gggggcaccc aaaggagcta tgtataccaa tcggtccata
    27781 gccaattact tgacagtttt ccccaaagac ttgctcagaa tcagagagtt ggaacattat
    27841 aaattaatat cttatcttcc cctggcccat gtctatgagc gatctgccat ccaattaggc
    27901 agtgttgcta ttaatgcatc ggtatctttt gttgaaagtc tggagaagtt tgcagagaat
    27961 ttaagggaag ttagtcccac ctttttcact gcagttccac gcatttgggg agtatttaag
    28021 caaaaaattg aacaaaaact gccaccagcc aaattgaatt ttttattaaa aattcctctt
    28081 atatccagtt tgataaaaag aaagatcatt cacagtttag gattaagcga gtgtactaat
    28141 tgttttagtg gagcatctca tctgccaata tccattattg atttttttga aaaactggga
    28201 atatatattc aggaaggata tggtcaatcc gagaatttgg cttatgctac cttatcgatg
    28261 ttaaatgaga gaaaacgtgg ttacgttggt accccgaggt tggaggttga gataaaagta
    28321 ggcgaagaga atgaattgct aatgaagtgc ccttgtctaa tgtcaggtta ttacaaaaat
    28381 gaacaagcaa ctaaaaatgc ctttacagaa gagggttggt tgcgaacagg agatgttgtt
    28441 gatgttgata atgaagacag agttaaaata ttagggagac tttctgagaa ttttaaaaac
    28501 cagacaggag agttcattgc tccttcgcca atagaaaaac tgttttcagt gaaccctatg
    28561 ttagaacaac tgtgcctggt aggtagagag ttacccaata acgttttgtt agttaccttg
    28621 aatgaagggg tgcgtttggg tatgccaaaa gaggagatca ctcactcatt gcaagaaacc
    28681 ttaaaacaag tcaattccaa attggttaaa tacgaaaaag tcagtcacat ccttgtgctg
    28741 aaagatgcct ggacaactga aaatgataaa ttaacaccta cactgaaagt aaagagaagg
    28801 gtagttgaaa aagattatgc cgagttaatc caaaaggcac tgcagcaatc tcagtcaatc
    28861 gtttgggaat gaggagacag tttatttaat aaagaactga tttcaaatat tcgttcctct
    28921 gctgacgtgc tgtctaaagt aaagcgcaga cgttgcggtc cttccatttg atagcgtttg
    28981 gcatgtactt gtattaagct aatcagggtt ccagggtcaa tcgatggttt ttcactaaaa
    29041 tccaatttac cttgttgagc actggcactg attttttgaa tgccgagctg ttccgctttt
    29101 aactttaatt cagtaatcaa aaacagatgt ttgactggtt gcgggagtaa accaaaccgg
    29161 tctatcagtt ctatttgtag ttctctaagt tgttgggtag tttttgcatt agcaattctt
    29221 ttatacatga tgagtctatt gtgtatatca cctatgtaat cttcggggat aatcgcgcta
    29281 attctcaagt caatttcagg tccttgatgc atgggtgctg acaattcagg agttttgcct
    29341 gcctttaaat cgttaacggc acggtcaagc atttccataa ataaattaaa tcctatggca
    29401 tgcatattac cactttgttc ttcaccaagg agttcacctg ctccacgaat ttccagatcg
    29461 tgtgttgcca aggtgaatcc tgctcccaaa tcttctaaag acacaatggc ttcgagacgt
    29521 ttcactgcat ctggcgttaa cagtttttca ttgggagtta ataagtaagc gtaggcttga
    29581 tgatgagagc gtccaacacg accacgtaat tgatgcagct gggctaatcc aaatttatcg
    29641 gcacgatcga taataatcgt attggcggtt ggaatatcga tccctgtttc aatgatggtt
    29701 gtgcaaacca ggacattaaa tcggtgatga taaaaatcag acatgatgcg ctctaattgc
    29761 ctttctcgca tttgcccatg tgcgctttga atttttgcct cgggcactaa agcttccaga
    29821 tcctggcaaa ctctttctat agtttgtaca ttgttatgca ggtaaaatac ctgccctccc
    29881 cttaatattt cacgcaatat cgcttctcgt atggtgagat catttttttc ttgccaaaat
    29941 gttttaattg ccaaacgctt ggcaggcggt gttgcaataa gtgaaatatc tctgattcct
    30001 gccattgcca tgttcaatgt tctgggaatt ggagtagcag tcatggataa aatatcaaca
    30061 tgagttcgaa gcgatttaat atgttccttt tgtttcactc caaatcgatg ttcttcatca
    30121 ataatcagta atcccaggtt tttgaaagaa atattatttt ggaataattt atgggttcca
    30181 attacaatat ctactttacc tgattgcaat gcggcaagga ctgcttcgga ctctttatta
    30241 ctgcgaaagc gggataataa ttcaatatta acaggaaaat ctgcaaatct atcgcgaaat
    30301 gactcaaaat gttgtcctgc aagcaaggta gttggagcaa ggatacagac ttgttttcca
    30361 ttttgtacag ctacgaatgc agcacgcatg gcgacttctg ttttgccaaa tcctacatca
    30421 ccacagatta ggcgatccat cggtcttggt gattgcatgt ccttaataat ttgttcaata
    30481 gcttgaagtt gatctggtgt ttctgtaaag gggaagccac tggcgaattt tatatattcg
    30541 gattgattga aatcgtattg gtgccctggc tgggcttctc ttttagcata gagatccaat
    30601 aattcaattg caacatcatg aatcttctcg gccgctttct ttttttcctt ttgccattgt
    30661 tcagaaccca gtttatgcaa aggggcgtgt tcactgtcca caccagtata ccggcttatc
    30721 atatgcagag aagtgactgg cacatagatt ttgtcatccc ctgcgtaagc aaggacaagg
    30781 aactcactgg gggtaccatt ggagtcaatg tattgcaaac cttgatagcg acctactcca
    30841 aattgaagat gtacaacagg agcgcccaat cgaagttcgg ccatatctcg tatgattaag
    30901 tcagggtcaa ctgatttttg agtgcttcgg cgttgaggtg tgctttgctc accaaataat
    30961 tgcgactcaa cgatgatcac aatttgtttg tcggttaatt cacagccata aatcaatggg
    31021 ccagtagtta tattgatggg ggattcatcg taaatgaagt ctaaccatga gttctgtgtt
    31081 tttggaatga tatggcttga tttaagcaga tccagtaaca cttcgcgacg ccctgcgctt
    31141 tcaacaacaa taagatagcg tcgagttgga tcagaacaat actcacttaa ttggcggagt
    31201 ggctcaatat ttttcctgtc aatgggtagt tgtggagctt tggcaatgtt gaaatttaag
    31261 gctccttttt tttcggaagg tttgtgatac aggcgtactt gttcataatg gtttgcttta
    31321 gttaataact cggtgggatt aataaagcaa ttatctgggg gtaaaatagg gcggctgaca
    31381 tcatgacgcc tttgttcata gcgttcattt aattctcgcc agaatctctc agcattctcc
    31441 tggatttctt caattaaaca aattttggca ttggctggca gataatcaaa aaaggtaacg
    31501 gttttatcaa aaaacaaggg cagataatat tctatgccag atggaaattg attgttacta
    31561 attgcttcgt atattgggca atggctggga tttccaggaa aagactctct gaaagctcgt
    31621 cggaagtgta attggctttg ttcatttaat ggaaattcac gagcaggtaa caaatggatt
    31681 tgattgattt tttctattgt gcgttgtgtt tccggatcaa attctctgag actttcaatt
    31741 tcatcatcaa atagctcgat acggaaaggt aaaccactac ccatcggata tacgtcaata
    31801 atagatccac gtagtgcgta ttcaccatgt tcaagcactt tattaacaca atggtatcca
    31861 gcttgttgca actgattcct gaacgagttc agatcaagct tttgcccctc tttaagcatt
    31921 aaagcgtatt gatttaaaaa ctctggcgga gaaagcctgt gcattaaagt gctggctgaa
    31981 gaaatgatga ttccattggt aatttgttga actctgctta aggtgtagag tctttctgaa
    32041 ataatatctt gatgaggaga gaattgatca taaggcaagg tttcccagtc gggaaaaaat
    32101 aataactcct gggtattttg gttcgggttt agaaaaaatt gtagttcatc ctgcaactga
    32161 ctggcgcata ggttgtcttg cgtaataagt aatttgatcc cgggtgtttg ttggcaatat
    32221 tcagctaaag caagtgccag actgctacca tagagttgcc cccatatttg tttatttttt
    32281 gtgggttggt aaagtagcgt ttttgtattc attggaattt aatgattaaa actgaaaacc
    32341 gcttagtata atgctaaatt aggtattctg caccataaag attttatttt agatggattc
    32401 agactcgacc aattctaatt gagttacttc gtgttcatcc aattcgatat cgaccgattc
    32461 aattttttgg aatctggaag tgatcttttt ggctgatgta ttgacttcat taacgtcctg
    32521 atgtgccaag tcaatgtgtt tggataattt atccattctc ttttcaaagc gttgaaaatc
    32581 attggccagg gcttgaagat gtttttgaat gatgtgtact tgtttgcgag ttgcatcatc
    32641 tttaagtact gcccttgctg tagtcagtac agccattaat gtgctaggcg ataccagcca
    32701 gacttttagc ttttgagaca tggcaattaa atcaggatag tttgcatgaa tctcagcaaa
    32761 tatagattct gcaggaataa acatcatggc gccatctgtt gtttcattgg gcacgatata
    32821 tttttcagcg atgtccttaa tatgcttttg aatgtcttgg cgaaattgtt gttgtaatga
    32881 cttacgatca gtggataagg catcactgtt gattaatcgt tgataagttt ccaggggaaa
    32941 tttggcgtct atgacaatat ttccagtggg ctcgggtaag aacaaaacac agtcagcccg
    33001 tttttgatta ctgagggtat attgcatttg ataacttgcg ctgggaagca tattgctgat
    33061 gagtgttgat aattgaactt caccaaaagc accgcgagct tttttgtcca ctaatacgtc
    33121 ctgaaggctg acaacatgac tggagagttc ggtgattttc ttttgtgcct catcaatgat
    33181 ggtcaatcgc tttaccacgt caataaaagt agacgaagtt ttctcaaaac cttcggtcaa
    33241 tttgtgatta acctgctgag ttagtgtgtt taaatgatta cgaatttcct cagttagtaa
    33301 ttgtaagtga gaagtgagtg agctcgcatg atgtttgaag ctatgagtca tttgctctct
    33361 gacatcggcc atttgtcttt gtatggcatc actaatgagc tgttgtgtag ccagttgtcc
    33421 ttgagctatc ttttcattaa taaattgttg gctcgtttga tatgattggc ttaaatcaat
    33481 atgtaactgg tgaagttgag agataagtgc ctgttgtatt ctttcaccaa gttgttcggt
    33541 cagttttttt tgctgtgctt gttttatcaa aacccagaaa agcaataaga attgaagggc
    33601 aatagccaac gctatgccct caaacaaagt gacagaagaa aataatgcca ttcattaatc
    33661 cactaactga ctaagcttga caggttggtt gtattgatac acagttttta actgcccata
    33721 gtctatcaag cgtaaataac ccagatagag gttatcgtcc gataattgat tgtgattgtc
    33781 aaaggtaaat tgcaaaacat gcttaccggg ttcaagccac tgcgctcttt gcgaggtctg
    33841 aataggcctg gcttcaccct ttccattttt ttggaacaac acactttgta aagcgtatcg
    33901 gcttgcagtt gcaacatcta cagtgacaac aaaagtcaaa ggtttagagg acaatttttt
    33961 tacattcata agactggctg agggaatgga gtatgaaaag gctgtatggc cagatcgtct
    34021 aatgatttct tgcccgtatt ctgtttgaac atcagtttct aagtaccagt tttcgccgcg
    34081 atcatttaac tcagaatcca aagtggcagt tccttcaaat acattggatt ttaacttggt
    34141 cagattaagc gaaatgactt gacctttagg gcctacaagt cgagcatcaa cgtcattgac
    34201 gtcatactca gtgatatcat tgtgaagcga tatggtcgct tttagcttat ccccatactg
    34261 gtaatggaga gaatcggttt caacctccag gtaagtgatg gaaaatttat caaggacact
    34321 aatcatataa gcatcggcat atttgttagt aatgtccttg ctttttagta taaattttcc
    34381 gtaccccagt tcaggtttta tttgtaacat agcctggtgt tttgtaatgg ctaaaggatt
    34441 attgccaacc tcgtcatcct gattatagag agaagaggct tctttcaggg gcaggagttg
    34501 gttttttggc gttttgataa gtaattccgg gatggattta ttttgcaaag gggtaatgcg
    34561 aacgacagcg ccaggagcca atgttgatat aataactccg cgttgtaatt gttccagaga
    34621 aatacgttct ttgtaaccat aacttctacg aacattactg atgttgttgt ttaatggttc
    34681 attggtaatt tcccatttat cgtgcaggtt ttctcgtgat aattggctgc aattctcaca
    34741 ttcataagct ttggttggct gtcttggtaa tgtaaacgca tgagcttggg caatacaaaa
    34801 tgcggatatc catataagat gggcagtttt cattttacaa ctccttcatt aactagggca
    34861 ctgataagta tttgatccaa accaaaacaa gaatgtcgac tttgattatg gcttaaaggc
    34921 aaattgtaat cagttttgtc tatatcatag aaccatactt ctcctgcttg agcttgcgac
    34981 tcacaatcca gaaagccatc agcacactga ttaagataga gtttggcgat ttttagcccg
    35041 ctatttagca tgtatccatt gcaatagctg gatgttgaaa atggtgaagc atagacatca
    35101 gtacccacta tgactttaaa tggaatggac agagagggtc ggcctttgct tcccaatagc
    35161 aactcttcgt tatagatcag catgtcgcct acgcgttgct gtttgactgc atctgaaaga
    35221 taaccgagta accaggccaa actcgtttca aatatgccgc catttaatac ttcatcagca
    35281 agaggagtcc ctccgctgga aggtgcaatg gcaatcactt gactgatttt gcgtttgaga
    35341 gtcatatatc ggccatcata agttggattt gagagaatcc aacgcatgat gttggcacca
    35401 tcagagtggg tgtagactgt gagagagctc acatttttat cgttaataaa ttccagaagt
    35461 tgattagcaa cacaattggc tgcgtcttca tgccacatat attggctaaa gtcacaatga
    35521 accacgtagt gattttcagg ttttgccagg gtttgggaga tactttgtat gaaatcaatt
    35581 ttccaatacc ctccataggc atcttctctg tgatctcgag taccatggac aaaagcaata
    35641 ccttgattgg aattagtgtc ggaatgagaa tggataaggc ttgaaaacaa taaaacaata
    35701 agagtggata aaatgcgcag aagttccatt ttcaaaatat ctccttcaat acagagacgt
    35761 atctatatca tggttctggc aaaagattca agagatggga ttgatgcgtt ttagtggatt
    35821 tgcgccgatt acttggttcc gaataaagag gcttccaaaa aaaagatttt gccttgaatg
    35881 attaaaccac tcatatttga aagaagtaaa aaaatgataa actgttgttt ttattttggg
    35941 aatcttcatt aatgaatgat caaggaatta aggaatcgat agaaacgctt aaagagcaaa
    36001 taagaaaata cgattatcac tattatgttt tagatgaacc tttggttccc gacgcggaat
    36061 atgatcgatg tttcaaggca ttgcaacagt atgaagagca atatccgcaa tttttatcgc
    36121 cagattctcc tacacagaga gtgagcggta ctccttcaga tgcttttatg ccggtagccc
    36181 ataagcaacc catgttgtct ttgtccaatg tgtttactac cgatgaatta aaagcattca
    36241 ttaagcgagc aattgagaaa ctggatgaac caaatcaaca actggtattt gcttgcgaac
    36301 caaagcttga tgggttggct gttaacatga cttatgaggg cgggatcttg actcatgccg
    36361 caactcgtgg cgatggtgct gtaggagaaa acatcacagc aaatattaag actattgctt
    36421 cagttccatt aaggctaagg gttagtaacc ctccaaaatt gatcgaagtg cggggtgaag
    36481 tctatatccc caaagccgat tttgaagctt acaacgcaag ggctagagag ctcggtgaaa
    36541 aaacttttgc taatccgcga aatgctgctg caggcagttt aaggcaatta aatcctgaaa
    36601 tttctgccag tcgtccactt gctatttatt gttatggtat aggggcttgt gaggattata
    36661 agttacctaa cagtcatttg gagcaattga atttattaaa agagtttgga tttagagtgt
    36721 ctccagaaac gaggagggcg gttggagtag aaggctgttt agattattac cagtatatgt
    36781 tagcgaaacg gaatcaattg ccatttgaaa tcgatggggt tgtttataag attgacagta
    36841 tctccttgca acagcaatta ggttatgttt ctcgtgcccc aagatttgct tgtgcccata
    36901 aatttcccgc tacagaagaa atgactcgtc tgatagccgt ggatttccag gtaggtagaa
    36961 cgggcgctgt gacaccggtt gcacgtttgg agccagttag tgttggtggt gttactgtga
    37021 gtaatgcgac tttgcataat tttgatgaaa ttacccgaaa agacattcgt attggtgata
    37081 cagttattat tcgccgtgcc ggtgacgtga tccctgaagt ggtttctgtg attttggaaa
    37141 agcgtcccgc taatgccaga atgattgagc ttcctaaaaa ttgccctgtt tgtggttctg
    37201 aagtcgtaag ggaagccgat gaagcaattg ctcgttgtgt cggcggttta tattgtaaag
    37261 cacaattaaa aaggatgatg tggcattttg cttctcgaaa agcaatgtat attgaaggac
    37321 ttggcagtgt tttaattgac cagttagttg atgagggtat tgtccatcat ttggcggatc
    37381 tttatgaact cgatttgcag actttagcta acctgccaag gatgggggag aaatctgcaa
    37441 aaaacctatt atccgctttg gaaaaaagta aaaaaacgac tttcaatcgc tttctttatg
    37501 ctttggggat cagagaaatc ggtgaagccg gcgcaagggt tttagcggag cactactgcg
    37561 atgtagagag cttgaaagcg gcaacgattg aggaattaat gactctgaat gacataggtc
    37621 cagtagcggc ttctcatata gtccatttct ttgcccaagc gcataatctt gaagtgattg
    37681 accgtcttct cgagttgggt attcattggc caaagcccga aaaaatacag gttaatcagc
    37741 aaaatccatt ttttggtaaa acagtagttt taactggaac tctgagtacc atggggaggg
    37801 aagaggcaaa ggcaaaatta ttagccttgg gagcaaaagt aagtggaagt gtgtcttcca
    37861 aaacggatta tgtagtagca ggaagtgaag ccggttcaaa gctgattaaa gcgacagaac
    37921 tgggagtagc gattatagag gaagacgagt ttttaaaatg ggttaattca tgagtagcta
    37981 gatctttgtt atttggttta tgatgggtat caatgaatca ggtgaagtag gtatttaaac
    38041 ctgtaataat gactgattgg cacgagggaa gtaagtgcgg cgatatttgt taattcaatt
    38101 catgttattt ttaatgcctt gctgtgtcaa tttggcttat ggcagttctt gggtattcaa
    38161 taatccttac ccagagagcg aggccaatga aaatatttat tattcttctt ttaatgaaca
    38221 gccaaaaact ctggatccgg cacgttccta ttcttccaat gaatatcttt tcatagccca
    38281 gatctacgag ccactgctgg aatatgatta ctataaaaga ccctatgaat taatcccctt
    38341 aacggctacc cagttaccta agattcgata tttggatgct aatggaaatc ctgttccggc
    38401 aggagataac tcaaaaccgg catacagtgt ctataccatt cagataaaaa aaggaatttt
    38461 atatcaacct catcctgctt tggctcagaa gaaagatggc tcttatcgat atcatcactt
    38521 accagaagat tatttggaag agcatgatat tggcaagtta tcagatttta aatacaccgg
    38581 tacaagggag ctgatcattg atgattacat ttatcaaatt aaaagattgg caaaccctgc
    38641 tgtgagttct ccgatatatg gattgatgag ctattatatt ctgggcttca aggagtatgg
    38701 agatagtctt cctaaaggat tggccaataa aaattctttt attgatttac gtaagtatcc
    38761 tttgaagggg gttaaaaaat tagacgatta tacctttgag attacattaa agggacaata
    38821 tacacaattt ttgttttggt tagccatgcc cttttttgct ccggttcctt gggaagcgga
    38881 cttattttat tcccaaaaag atatggatga taagaactta tcttttggct ggtatcctat
    38941 aggtacaggg ccttttatgc tgactgaaaa taatcccaat agaagcatga tattggagaa
    39001 aaatcctaat ttcagagaag tgtattttcc tgtcaatggt tcagatgccg atagacgggc
    39061 aggttattta accaatctgg gaaggcgctt gcccttaatt gataaagccg tttataccct
    39121 tgaaaaggaa tccatacctc gatggaataa attcttgcaa ggctactatg atatgtcggg
    39181 gataagtgct gacagttttg atcaggctat tcatattaat cgctttggag aacctatcct
    39241 tagccaggaa atgaaagata aaaaaatata tttaagacaa accctggaac cgtctatcta
    39301 ttatactggc tttaatatgc ttgacagcgt tgttgggggc aatagcaaaa gagcacgaaa
    39361 attacgccag gcaatttcta ttgcggtgaa ttttgatgaa aatattgcca tattttttaa
    39421 tggccgaggt attccggcgc a
//
LOCUS       DACTJE010000009        38977 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.009, whole genome shotgun sequence.
ACCESSION   DACTJE010000009 DACTJE010000000
VERSION     DACTJE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 38977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38977
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.009"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..446)
                     /locus_tag="I9760_000340"
     CDS             complement(<1..446)
                     /locus_tag="I9760_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAT5042135.1"
                     /translation="MILDRTDKKILEILQANCEINNQELADLVALSPSSCLRRVKLLI
                     DNGYIKKQVALLEPEKVGLKLTVIVLVGLNNHQPEVMNQFEKTIQLFPEVIQCYLITG
                     QSADYLLKIIVPDLNAYQAFLLGKLTRINGVASVHSSFILRNVSDTT"
     gene            647..1879
                     /locus_tag="I9760_000341"
     CDS             647..1879
                     /locus_tag="I9760_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042136.1"
                     /translation="MSVSRVTAWFVWVIVSIFYAYQYVLRVMPNIMLDDITQQFHIDA
                     IIFGQFSGIYYIGYSLMHLPIGIMLDRFGPRKVMTGCIMLTVIGLLPILFAENWVYPV
                     IGRALIGIGSSAAILGTFKIIRMTFKEKHFPRMLSFSVMIGLVGAVYGGGPVSYMCQL
                     MGYKMVVEIFVGIGIVLAGITYLIVPDAQPSRQDSVISNIRMVFGNSKVILLCCFAGL
                     MLGPLEGFSDVWGSGFLKMAYSLNANAANYLPSMIYIGMCFGAPILSLIAEKTGYYLG
                     TIIASGVVMFLVFAMLVGGVLTIPSITISFILVGICCSYQILAIYKVSTYVPDNLAGL
                     TTAIANMIIMIFGYGFHSVIGYVVNMYGGTHVARAFVYGVGVIPLTLAVGVVGFLFLA
                     YQEKNFTPDASFASPELR"
     gene            1934..2248
                     /locus_tag="I9760_000342"
     CDS             1934..2248
                     /locus_tag="I9760_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HAT5042137.1"
                     /translation="MYMLYFHVPETHLDEVKNAVFAAGAGYVDNYNHCAWQTLGEGQF
                     MPLPGSQAFIGEINQLEKVPEYKVETICARERIKEVVAALKKAHPYESPSYQVVRIET
                     EF"
     gene            2439..3992
                     /locus_tag="I9760_000343"
     CDS             2439..3992
                     /locus_tag="I9760_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HAT5042138.1"
                     /translation="MLKILALLSCLILSSCLVGPNFKEPKKDAANYWLTSSPAIKQSA
                     FQNANWWKVFNDPTLTSLIDNGYHNNLSLQMAGVRVLQARAQLAQSVGELYPQQQAMV
                     GDYNYNRIGGNQLQGLLPPDFLTASLGFSASWELDFWGKYRRAIQSNDALFLASLAAY
                     DSALVTLTSDIASTFIKIRTSEELIKVTKTNIQVQAMSLKIAKDRYYGGQTSLLDVEQ
                     AATELAETQSKLPQYVSDLQHQKDILAVLLGTTPDKVDDLLKKNKGIPKALAVVEVGI
                     PKEVIARRPDVYQARMEAIAQSAAIGAVKANLYPSLSLVGTFVFASNNIGNSTISDIF
                     NWSNRNIVAGPSLAWSLLNYGQITNAVRAQDAAFQQALLNYLNLVLKVQQEVQDNITR
                     YVEGQKTVSSLKTANAAAIESTRLALVRYKEGEANYTTVLNAEQQQLRVQTSLVNATG
                     ELALSLVGLYRSLGGGWQIRGKNDIVSNQIKSEMAARTNWGSLLKQENHLPPDSNGQR
                     MKQLYLPTW"
     gene            3998..5152
                     /locus_tag="I9760_000344"
     CDS             3998..5152
                     /locus_tag="I9760_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAT5042139.1"
                     /translation="MNSQNKQNLVKTVAIVFFGIVFVYLVYDHFTRAQVATPAPPDVI
                     VQKPKLMKMGQYVTQTGNTVAFQSVDLVARIEGYLDAIKFTDGAFVKKNQELFIIEPE
                     PYLEQLRAAEAKVAAQKANYAYAKSEYARQQRMYKQNATSLNSVEKWKAKVEEAQAEV
                     DKSEADATNAAITYGYTHVSAPFDGRMGRHLVDVGNLVGHGAATDLATIEQIDPIYVY
                     FNLNELDLIRIRNAARKRGYKPMELVKIPVEVALQDEKGFPHKGTLDFVNTGLNASTG
                     TMEFRALLPNKGYPLVPGLFVQVRVPISEPSPKLTIPDTSVQYDQIGPYVLVVDKDNY
                     VLTKRVTLGGLENGVRAITKGLDAQDSVIVSGLQNATPNHRVTPKTEQPE"
     gene            5212..8364
                     /locus_tag="I9760_000345"
     CDS             5212..8364
                     /locus_tag="I9760_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAT5042140.1"
                     /translation="MISKFFIERPVLANVIALFIVFIGIIAIAVLPVSQYPAIVPPTI
                     QVTTTYPGADAKTLINTVALPIEQQVNGVEDMLYMQSTSTSGGTYTLIVTFAIGTDLN
                     YAQVLVQNRVQAAMAQLPESVQKQGVIVQQKSTAILQFITLTSKNNEYDGVFLDSYAT
                     INMQDELSRLPGVGNVVVFGSGSYAMRVWLDPKKMLAFSLNPSDVLNAISYQNKEVSA
                     GQLGAPPTVGKQAYQFTVNVPGQLSDPKEFENIIIKTIDTPPDQNSSASSSAQVVRIR
                     DVGRVELGSESYNQLANLNGKPTAAIGIFQLPGANALDVAQEVRKAVAKMAKQFPPGL
                     EYSIPFDTTVFVKASIEEVYKTLFEAGILVLIVIVVFLQNFRASLVPATTVPVTIIGT
                     FFAMLLLGYSINLLTLFALVLAIGIVVDDAIVIVEGVTQHIEKGLSPKESAILAMKEL
                     FGPIIGITLVLMAVFVPAGFMPGLTGSMYAQFALVIAATAFISAINAMTLKPTQCALW
                     LKAPDTSKPKNIFFQAFDRIYNPIESAYVKFIDRLVHRSGKVCLIGIILVACAIYGLT
                     RIPTGFIPMEDQGYLMLSIQLPDGASLGRTDEVVSRLAKKASEVGGVDNVIAIDGISL
                     LDNNSLLSNAGVIYVIFKDWSVRGKSENLRALYTKFNAIAKETLDAKVLVVVPPPIQG
                     LGMSGGFQMQVELQDGTFDYRKLQQATDQMINTGRQYPQLQNLMTTFRASVPQVAAPI
                     NRTKAESLGVRVADAFDTLQTYLGSSYVNLFTKFGQVFPVYVQADASSRVSSEDLRNY
                     YVRNQSGSMVPLGTLTDVGPAVGPSIISLYNLYPSSNINGVAARGYSSGQGIQVMEKL
                     AKEQLPPGISYEWTSTAYQEKVAGNLSYFIFALSLVLVYLILSGQYENWLIPSAIILS
                     VPLTLVGTVLALGSLGMDNNMYTQIGLLLLIALATKNAILIVEVAREQREIHNKSVLE
                     AAVIGAKTRFRPILMTSFAFIMGVMPLVFATGAGANSRRSIGIAVSSGMLASTCLAVV
                     FVPVFYVLLQTWQDKRKAKH"
     gene            complement(8730..11483)
                     /gene="sidC"
                     /locus_tag="I9760_000346"
     CDS             complement(8730..11483)
                     /gene="sidC"
                     /locus_tag="I9760_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidC"
                     /protein_id="HAT5042141.1"
                     /translation="MVINMVDVIKFKEPERCDYLYVDENNKVHILLPIVGGDEIGLDN
                     TCQTAVELITFFYGSAHSGVTKYSAEHQLSEYKRQLEEDIKAINSQKKISPHAYDDLL
                     KEKIERLQQIEKYIELIQVLKKQYDEQNDIRQLRTGGIPQLPSGVKEIIKSSENAFAV
                     RLSPYDNDKFTRFDDPLFNVKRNISKYDTPSRQAPIPIYEGLGYRLRSTLFPEDKTPT
                     PINKKSLRDKVKSTVLSHYKDEDRIDGEKKDEKLNELITNLQNELVKELVKSDPQYSK
                     LSLSKDPRGKEINYDYLVNSLMLVDNDSEIGDWIDTILDATVDSTVWVAQASSPFYDG
                     AKEISSDRDADKISIRVQYLLAEANIYCKTNKLSDANFGEFFDKEPHATEIAKRVKEG
                     FTQGADIEPIIYDYINSNHAELGLKSPLTGKQQQEITDKFTKHYNTIKESPHFDEFFV
                     ADPDKKGNIFSHQGRISCHFLDFFTRQTKGKHPLGDLASHQEALQEGTSNRLHHKNEV
                     VAQGYEKLDQFKKEVVKLLAENKPKELLDYLVATSPTGVPNYSMLSKETQNYIAYNRN
                     WPAIQKELEKATSIPESQKQDLSRLLSRDNLQHDNLSAITWSKYSSKPLLDVELNKIA
                     EGLELTAKIYNEKRGREWWFKGSRNEARKTQCEELQRVSKEINTLLQSESLTKSQVLE
                     KVLNSIETLDKIDRDISAESNWFQSTLQKEVRLFRDQLKDICQLDKYAFKSTKLDEII
                     SLEMEEQFQKIQDPAVQQIVRDLPSHCHNDEAIEFFKTLNPEEAAKVASYLSLEYREI
                     NKSTDKKTLLEQDIPRLFKEVNTQLLSKLKEEKAIDEQVHEKLSQLADKIAPEHFTRN
                     NIIKWSTNPEKLEESNLNEPIKSVQSPTTKQTSKQFREAMGEITGRNEPPTDTLYTGI
                     IKK"
     gene            complement(11634..14360)
                     /gene="sdcA"
                     /locus_tag="I9760_000347"
     CDS             complement(11634..14360)
                     /gene="sdcA"
                     /locus_tag="I9760_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdcA"
                     /protein_id="HAT5042142.1"
                     /translation="MMNMVDKIKFKEPERCEYLHIAKDNKVHILLPIVGGDEIGLDNT
                     CETTGELLTFFYGKTHGGTKYSAEHHLNEYKKNLEDDIKAIGVQRKISPNAYEDLLKE
                     KKERLEQIEKYIDLIKVLKEKFDEQREIDKLRTEGIPQLPSGVKEVIKSSENAFALRL
                     SPDRPDSFTRFDDPLFSLKRNRSQYEAGGYQRATDGLGARLRSELLPPDKDTPIVFNK
                     KSLKDKIVDSVLVQLDKDFNTKDGDRGQKFEDIKKLVLEEYKKIDSELQVDEDTYHQP
                     LNLDYLENIACTLDDNSTAKDWVYGIIGATTEADYWPKKESESGTEKVSVFYEKQKEI
                     KFESDTNTMSIKVQYLLAEINFYCKTNKLSDANFGEFFDKEPHATEVAKRVKEGLVQG
                     AEIEPIIYNYINSHHAELGLTSELSSKQQEEITEKFTQRYHIIENSPHFDEFFVADPD
                     KKGNIFSHQGRMSCHFLDFFARQTKGKYPLGDLAGHQEALQAGTSNRLHHKNEVVAQG
                     YEKFDQFKKEVVKLLAESKPKELLDYLVATSPTGVPNYSMLSKETQNYIAYNRNWPAI
                     QKELEKTTDIPENQKQDLLRLLSRNNLQHENLSAITWSKYSSKPLLDVELNKIAEGLD
                     LTAKIYNEKRKSEWFKGSRNEARKTQCEELQRVSQEINALLQSESLTKSQVLEKVLNS
                     IEALDKIDRDISAEYNLFKSTLQKEVQSFRDQLKDICQLDNYAFKSTKLDEIISLEME
                     EQFQMIKDPAVQQIVRDLPSHCHNNEVIEFFMTLNPEEAAKVASYLSLEYRELNKSTD
                     KKTLLEQDIPKLFKEVNMELLSQLKQDSAVKEDVYEKFCQLADKIPPEHFTRNNIRKW
                     SANPEKLEESNLGELLKSSEGSITEMARKYRETINEMTGRNESLRETVRNTI"
     gene            14611..15804
                     /gene="sdeD"
                     /locus_tag="I9760_000348"
     CDS             14611..15804
                     /gene="sdeD"
                     /locus_tag="I9760_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdeD/laiF"
                     /protein_id="HAT5042143.1"
                     /translation="MLYNKSSFCLWKETIVPIILDSDVLEVAEYVYKTRLSQPYTEVG
                     SEWEYNYKNPTATFAKGDGHNLQRYITIDGKQLHRPIHGLAHTMRTLMYSQLMYCSSK
                     KQPSPHVCQDGRTIADLSELDLKKINIAQLFFVAGRESEASYGDAYHRYHLYGAKQFE
                     EYARKHLTHLFSEEEIRLYSRCIEDRVGDSFDGTPEGYIIHLSHMIDLMRCKSPVEVF
                     LGHSKGVSGIVPTLIHLFGKQDGLDIMHYARGLFAATGEAVPYIDSSEWPHLGVDLSR
                     VQRALSIVGDINVPGQEADSKKTAQAGFSVDGCYSALTSVPTPSWYEKELKEIDDEKI
                     DVKEVDDREIEKEHEIVVPSQATTPLSTVKTDSFVEKLLKPFMIWKKPEVQTTQPTTE
                     KTNKP"
     gene            15875..18298
                     /gene="sdjA"
                     /locus_tag="I9760_000349"
     CDS             15875..18298
                     /gene="sdjA"
                     /locus_tag="I9760_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdjA"
                     /protein_id="HAT5042144.1"
                     /translation="MFSYLDKLLDGIFGYQKSDTSQLSTSTPPTLTTVPLKQEYFVKI
                     GESETQDLGLLPVVSKRHNQSVPLEEIPFEDTRLELIELYIALLKQCVLDEKLKSIPA
                     QYLISHYLFIKTLAANEGNKGRKDLYLNLSQKVADYLEKNESKIWSMAVECAKTSEYP
                     IVDWIKKHHLHFNFIRAFILDYNKKSLTHNQRAFMQQFRDSAAFFFPDQVYLAWLTQS
                     YEPGSILNPMYRESRSTHYYHANITDNLLLRTRPKQVNFGPQHFFQKGKGPVKNTYRF
                     NINDGKLMRIQGRTLLFSTNKGNEVIAVKVQKKGEPQSALSNEFQMADYLLKHQRRLN
                     LQSQLPTPLSQYSINRTEILEKCSKSPDFEKFKNLISDAKSLEIYVYKATPSYFTYLH
                     DKQQSFSQLTSSVQKNVHDLFVLLREGIVFPYLADMFHTHIDESKRSDKGRYQTLVEL
                     LSALQSQMGRLDKWQKAVEFVNLRASGIADLGDNLPLTSFLTVSDWTKHYHAELLTGV
                     YHPSFLFLDKSSGSVRSLFNSRRKIFGNYLYLNIIAEYLLVIQLVIGCYGDKVTRKMN
                     SKSKAKVWEHLAELMFSSCAEAVNLITGMPKSRALAFLKQRANVRKHTQQTSFWMTPD
                     YSNLDRLSVQSQQSTLYPGESDYEINSDLISGVGLSLDGINQDLGDYNQASPLRELEK
                     LLYATVTLIEGTQQLDKQFFQQLDETEKMILSAAGVDKCYQAVAKLLDLARPGCQMQR
                     RLAFTYYEMIKRIYPCSNPADVRFELVAKEEAIIKIQRFWREHKKENQSLEKGFDFDR
                     NTSSSQSPL"
     gene            18697..19392
                     /locus_tag="I9760_000350"
     CDS             18697..19392
                     /locus_tag="I9760_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HAT5042145.1"
                     /translation="MPEETSTNLPKSLSHFLIQSDLDYKNSLSSIPLFDVKRTIFWDD
                     NLRKMFASIFYHLRGHFINFLWYVANFADNEASKQIIIQNIYEEIGIGNRFSHEKLYE
                     RFAKECGVDIHDEIVNETNYIPFAKEFNKAHLSWLAQHDPKDHLCAFAAYERLDNLDY
                     PFLVELAKSMKISQHGMTFFNVHTHVDHFDTTLQLIVPIWEESPQRLIESFHFIQTHQ
                     LGMWEQFSNALFN"
     gene            complement(19440..20702)
                     /locus_tag="I9760_000351"
     CDS             complement(19440..20702)
                     /locus_tag="I9760_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT5042146.1"
                     /translation="MQRLKNIFTAIYQYLLKETEDASHQITLFGIVMMINYPLFGVFW
                     KLEHFQLTEEFILRITAALLCACLAFNQFWPRQLLKFLPVFWYIVLLFCLPYFFAYLT
                     LINNGSTLWLMNCVSAIFFLLLVSSVLGALILLISGVGLAFFHFYILSNNQFVYIPGT
                     ISLFSLIVTFIAAIIIGALFARDREITYAGRLSGMRMLAGSIAHDLRTPLASIYLQAE
                     LQELIVERLNNPEVQKDLKENLSKITRGIEMSNQLIRMQLNNIQRDKLDTSTFSIYSI
                     KKLLKASLEEYPFKENQKSLIHLNDKNDFSIWIDEVGFKNMMWNLLKNSLEYIEETHK
                     GEISIWLEQGWEKENFNYLHIKDTAKGLNHKEYEKIFDFFYSGRKEGTGLGLAYCKLL
                     MQAAGGDISCKGKKNEFAHFTLKFPKID"
     gene            complement(21099..21986)
                     /locus_tag="I9760_000352"
     CDS             complement(21099..21986)
                     /locus_tag="I9760_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2505 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042147.1"
                     /translation="MIKGKLMPNSLKDVQKLICDEGITDNVITTLSRLKPFDLAMLKA
                     TSDNKVKTLLDSDELKPFWVNKFNKLRLEKDHIFQFRNPDPQSRADFYCGYVLYLAAL
                     KEKQKEISSYYDYLNLSFTTFNCFYAAQEILTFLIGACKNDTKRENIDLLYNFVTSQS
                     TQIQEHKTPGCLLLANAYFYLAGFYLSLDLKAESIECYKECWGQLHLAQLLETDSERE
                     IHNAYFNKGLATSNAFGLNSISEIKARCLDLASEALPYPARNVMEANAVKTFENRFKD
                     SMSTAGKDDERSITRPIIL"
     gene            complement(21988..24816)
                     /gene="ceg32"
                     /locus_tag="I9760_000353"
     CDS             complement(21988..24816)
                     /gene="ceg32"
                     /locus_tag="I9760_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg32/SidI"
                     /protein_id="HAT5042148.1"
                     /translation="MTKIYLLTAPFDEEAKTGDGQYAASLELGFAENYEHIPCKWLKK
                     DKGDYSIPFPTGTTSIPEETIPSAIVLQPVANENTVISGYKLKDTVSSPEKAQEVNNK
                     TVSPRTPKIIVKHDNSLQSLTIMDIYSQKPIQFDESKVDEIIHSLETKKVNLEKAIED
                     NNAELSKIKKQKSKLAYLTRLYKENKENIQDYCTLNEYIEAHLFNPKFLSRHEKALNN
                     FKALKSQFTGPVNLKELEKLTDKLTGIKEYSYDFHSNSLPYDLEHDKSFRNFYDFDGL
                     KESIESIIKELEVLNSIRQAVSDKYPNSFKALNETEEHDDKLKFINIIFNDGFSTTYD
                     QQTFIKALSALDIEKAIDAYTNVKNKLENTQDIIANKEGCRNKLISELQTLIANKQEP
                     YLSANEKLGGFYSKRKLSASEGFHLAYQANRRDPIKPEVIENIITKMKPIDEDTHLDI
                     HIRPPDCGVFITPEDIKKFQEAGIKVNITIHEYKQNYTRRYLQQYTHDLMRQANSVQF
                     FNAEDRENAIIAATYGDCDKRNTTEPTGVAKKIREVGEDFDLDKYPVQKYDLKGKSGL
                     TVASQKLSTEPDHPLDVVAKAPNILSFGTIRPGKGFEEALKLAQLIKDNSLSIHEKIK
                     RVPIVKLAGDPQDKALMKQIVVERFGKTAVKTYQKTHPYDNRFNNSQRRDYWKNLVRE
                     LNAKVKEEVAVLNNPYIEIYPWCEPHELLDLKQNCKYVCRMDDMGMRNNGSAIISVLD
                     VGVVYTKFGSVTDDIFIKGGKYGNAVDIGEYRYGKYSLLKKEKEFKEQHEEEPLPKWL
                     IKNPDSAYKRQSESRDPKDILDSIVAREENQLICDNIEDSDNYRTVVEAQKLLKERFT
                     LKNAVDHLLENIGLGHLIAQEEVDELFETVDPVQAQIDNLDILSDIKTPRLCLSRSCP
                     ELGFFGSRRGDLEAKQENNKLKESILVF"
     gene            25124..25444
                     /locus_tag="I9760_000354"
     CDS             25124..25444
                     /locus_tag="I9760_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042149.1"
                     /translation="MKPLSRKLGYWEELCQWRTILIYDQHGNSHEILLIAGHNIGDGV
                     SVAYFFHTLFRIYHKSDSNPSLPLYPPVEELVQHKKGKNLLCLLHFCLLRFITRIMSL
                     LASE"
     gene            25852..26100
                     /locus_tag="I9760_000355"
     CDS             25852..26100
                     /locus_tag="I9760_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042150.1"
                     /translation="MGVTVTNIGLLPFNTQYSHLKWKKSYFCTSRQNADIPVLLNVLT
                     LNEQLMFCFTFTEPMCGSVFQENYIKNFISNMGKLTDC"
     gene            complement(26097..27329)
                     /locus_tag="I9760_000356"
     CDS             complement(26097..27329)
                     /locus_tag="I9760_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HAT5042151.1"
                     /translation="MTFYMPKSHNSIIFLGLLSAFSLLTFDLYQPSLPFITNYFNITP
                     SMGQLTLSIYLLVYGITHLLWGPLIDHFGRRRLLPGNLLLALIGSLMCAFAPNITMLI
                     CGRALQGFALCCANLIAYSAARDFEDTIVRAKVLSYISMTVSVSPIIAPVIGALIFHH
                     FGWQANFIVMAIVACILLIQTKLSLLESPFWTPPQERFSVKKILQEYKSILNIPSLWC
                     ASFILMFGISAVMLTVINSSYLIIEVLNYSPLAYGLIFILNGLNIIFGNYLGIWLRDR
                     LPIITTIYLGNWFIVLGGTAMLVTSKLFGFSLLALSFSLIANLGISVSAAPTISIALT
                     DFKQNAGIVMAFMNTIRLIGPSLLTILTGYLLTRNLDALPLGLIGSGIGALFFSWHFS
                     RLTTESRNSDMDSEEALT"
     gene            complement(27414..28151)
                     /locus_tag="I9760_000357"
     CDS             complement(27414..28151)
                     /locus_tag="I9760_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HAT5042152.1"
                     /translation="MPKKLLIAALLCNIFCNPSHLYASSTEIPILGKTMTQAKQQQMT
                     PKQALQRLKDGNQRFLSNQPLARDYLKQAKQSAYGQYPFAVILNCMDSRSVPEFFFDQ
                     GLADLFTLRVAGNVLNDDILGSMEFATKVVGARLVVVLAHTSCGAVAGACKDVKLGHL
                     TDVINKIQPVVKPSMESTGIDNCSDPKLIDDMAKANALHVVKNILEQSPILNELVKNK
                     QIGIVAGLHDIKTGKVTFFEEKRSVPE"
     gene            complement(28191..28763)
                     /locus_tag="I9760_000358"
     CDS             complement(28191..28763)
                     /locus_tag="I9760_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAT5042153.1"
                     /translation="MLHNTKINKLAVFCGSNTGYSEVYKQSAENMADVLSSNGITLVY
                     GGSKSGLMGIIANRMLKNGSNVIGVIPKSLVDTEKAHDGLTELHVVNSMYERKILMCK
                     LSDGFILLPGGSGSLDEFFEMFTLVQLGYHKKPCCVLNASGYYDYLLQFLDYAVNQGF
                     LRSKDRNALIVNQYPDTLIENLFEVINNRK"
     gene            complement(28893..29117)
                     /locus_tag="I9760_000359"
     CDS             complement(28893..29117)
                     /locus_tag="I9760_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042154.1"
                     /translation="MSKFLVFIGSILFFSSVLAGDIPEVDIQDQQSDQELCAQKFYNQ
                     CINKCEKTNYGDCTQACEENAKNQCLYAGE"
     gene            complement(29431..30363)
                     /gene="mavJ"
                     /locus_tag="I9760_000360"
     CDS             complement(29431..30363)
                     /gene="mavJ"
                     /locus_tag="I9760_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavJ"
                     /protein_id="HAT5042155.1"
                     /translation="MTITHTAAGGITMTLELIRNGETDTNDYEEHLDSFGYETLEKSL
                     KEDAKSICQFFCNEELVLQGLKKLVTQFYQFNQPEKKTLMQFFDGWGSKNHFNQGTTS
                     NEKIPFETSRNFHFPNHTPVLCGELTADLFNNVLLKQGYLSADIGAGPKHGKWAHSIQ
                     FFLLEEARKAGQLNLHANNVCEFIKKISQIHGQYESLSLWSILFDSFDETFTCPNLIT
                     QTLTSQWDNSKEAQFLADRLNSFQKKFEKAASTENNYNAYANKKYLSKIDKANYVKYK
                     DKCAILWFAPQDKKTIPKPSSQEELKQNQLSFLN"
     regulatory      30505..30608
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            30595..30861
                     /locus_tag="I9760_000361"
     CDS             30595..30861
                     /locus_tag="I9760_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042156.1"
                     /translation="MSIVIFSGIVAFITSFIGLLPQIVKSLKTKSTEDLSMMMLVNYL
                     VCSLAWIIYGSSTNSFFVTSSNVVGLIVSFLLILLKRRYDARCN"
     gene            30845..31495
                     /locus_tag="I9760_000362"
     CDS             30845..31495
                     /locus_tag="I9760_000362"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAT5042157.1"
                     /translation="MLDVINFKDYRSILCLNGDLPDPSFFHKRKLPVIAADGAANVLC
                     NLGVFPDLITGDLDSIQPALLENHSFLHLPDQGSTDYQKAMHYLQTHDLLPAIVVGIN
                     GGYLDHILNNINIFMDTNCLLYSPPLKGFVVNEKLRVNFILPVQTKISLIGIPEVVLS
                     SEGLKWELKHSHLSFPGKNSCFNRTQTAEISLEVHQGAALVLIYEDAIEDAGVSSL"
     gene            complement(31654..32103)
                     /gene="trmL"
                     /locus_tag="I9760_000363"
     CDS             complement(31654..32103)
                     /gene="trmL"
                     /locus_tag="I9760_000363"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAT5042158.1"
                     /translation="MIHIALYQPEIPPNTGNIIRLCANTGAQLHLIHPLGFTLEDKQM
                     RRAGLDYHQWASILHYENWQTFLQSHSQRRLFCCSAKGKVKYSDVDYKADDILLFGPE
                     SCGLPQQILNQYSTIRIPMRENNRSLNLSNAVAIILYEAWRQMDFNM"
     gene            32381..33799
                     /locus_tag="I9760_000364"
     CDS             32381..33799
                     /locus_tag="I9760_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine synthase"
                     /protein_id="HAT5042159.1"
                     /translation="MDKNHNTKKILFKNRFVILGFGCVGQALMPLIFEKFDIKPSQVT
                     IIAAEGTKVDVAQQYGVSFKLQQITPQNYLEVIGSTLEENDFLIDVSIGISSLALIIL
                     CNQKGALYINAATEPWKEEFVMEKMALNRRTNYSLREEVLRLKDKTQKTALITHGANP
                     GLVSHFIKEALLNIAKDNGLTINRPKNAAEWANLAMTLGIKVIHVAEQDSQVTYPPKS
                     PGEFVNTWSANGLILEGLQPAEIGWGTHEAHWPHDAYSHSNGPQCAIYLSRPSAGVMV
                     RSWTPTLGAFHGFLITHAETISLTNFLTLKNGSELLYRPTVHYAYNPCPDARLSIFEL
                     KSNEWKPQNKNRLILNEIIDGCDELGVLLMGNQRGAYWYGSTLSIQEARQIAPYNNAT
                     SLQVVASMISGIIWAIEHPDEGIVEPEEVDHQYIIDIAKPYLGKVGGYYTDWTPLKNR
                     GELYPEEVDLSDPWQFFNIRVN"
     gene            complement(33850..34542)
                     /locus_tag="I9760_000365"
     CDS             complement(33850..34542)
                     /locus_tag="I9760_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HAT5042160.1"
                     /translation="MTMKFPFQIIDLTHSVSPESPGWDLDCGFTHSNFIDYEDCTSEI
                     KFRVQQIHSPLGIGTHMDAPAHCITGAASITDLDLHRNYLLTQSVLINVSHQTDEHYQ
                     ITKQDILDFEARYGRLKKNVFVLFYTGWDKHWPNPDKYRNNLVFPSISAHAAQLLLER
                     NIAGIGIDTLSPDTADSGYPVHQLLLQAGKYIIENVANAHSLPPIGAWTLALPWKIIN
                     ATESPVQLIGLV"
     gene            complement(34910..35404)
                     /locus_tag="I9760_000366"
     CDS             complement(34910..35404)
                     /locus_tag="I9760_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAT5042161.1"
                     /translation="MGSLRKHQPGKTTDLLMSPFLRLQQEVNHAMSDFYNLFDPQRSA
                     NLEQFEHISLTPSLDIVEDKDNFKIEVEMPGMGEEDIKVSFCENRLTIEGEKTTSKKD
                     ENKNYISREISYGRYERTISLPLSADVDKATASFKKGMLWITIPKKTEAKENVKTIKI
                     EKAK"
     gene            complement(35667..36653)
                     /locus_tag="I9760_000367"
     CDS             complement(35667..36653)
                     /locus_tag="I9760_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HAT5042162.1"
                     /translation="MHAMIMEKPGCELQYQEVNKPNPNENELLIKVSACGICRTDLHV
                     VDGELKNPKTPLIPGHQIVGTVEQVGSAVKKFAIGQRVGVPWLGGSCGICNFCTSGRE
                     NLCDQACFTGYQIDGGFAEFCVANYHFCFPIPDGYPDHQAAPLFCAGLIGYRALLKAG
                     DATHLGLYGFGAAAHILIQVACQKGIKIYAFTSPGDQETQRFAYRLGAVWAGDSTQSP
                     PHLLDAAIIFAPVGALVPQALSHTVKGGTVVCAGIHMSDIPSFPYEILWGERTVCSIA
                     NLTRKDGEEFLSLAPKIPVKTKVQTYPLTEVNRALDDLRHGRYRGAGVIVMK"
     gene            36915..37478
                     /gene="thpR"
                     /locus_tag="I9760_000368"
     CDS             36915..37478
                     /gene="thpR"
                     /locus_tag="I9760_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAT5042163.1"
                     /translation="MNAIRAFFAISLPPAIQENIRSNLEPLRQMAPVHSIRWTKPMHL
                     HITLQFLQRLETKHIQVLIERVSNELNHIKQFDLEFGVLEWFPSPKNPRLISLTVDPQ
                     DRLRDWSRMIGQVMDSLKYPLEKRPFRGHLTVGRVVNFSPKLQQLLQIKFPIIPPVPI
                     NKFYLIESRPDKEGSNYFPLAQFDLTR"
     gene            complement(37601..>38977)
                     /locus_tag="I9760_000369"
     CDS             complement(37601..>38977)
                     /locus_tag="I9760_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HAT5042164.1"
                     /translation="LQLQLKSLRKQIQEQTLVVEGLKEELQKQKKSNTHQEETIERIT
                     KEKSLADSALESLRKEMYELTRKNEENQLKLTKQVHSLSEQLEEKQLQIREFEKQLQE
                     KEKRVEQSEKGKASAKRTVASLREQVSNLKLQLQQLGEVIQEKEKGSSLISQQSKQII
                     ALQEIIEDQKRQLEELKIKIQELVSANQELGKQNQSLSKENLHNKNTVEDLKKKLNEL
                     NVQLEQLHQSSNEKEQTIRKLREELIKKDSSLKQNEEMQLAQKHLQEEIDRLQKEIKQ
                     QQLNTNQLESIIAQSKEAEKRYQQALQQKKGIYFARMERVSPIYLQIQQIEQKAKELE
                     ERRETEASTAAKTLATKLRLEIKNYLDNNESDEKSALNSFKINAKRHIENSKETLNQH
                     REEWKYLLANVTLGVFLLGIGYLAAILINKATTGNYTFFSQTNSGKKLDALEKAISST
                     HSETLVYG"
BASE COUNT        11471 a         6959 c         7410 g        13137 t
ORIGIN      
        1 gtcgtgtcag aaacattgcg taatataaag ctggagtgaa cacttgcaac gccattgatg
       61 cgtgttaatt tgcctaataa aaaagcttga taagcgttta aatcaggcac aattatcttc
      121 aataaataat cagcagattg gcctgtaatc aagtagcatt gaatgacttc cggaaaaagt
      181 tgtatggttt tttcaaattg attcataacc tcaggctggt gattgtttaa ccctactaaa
      241 acaataacag tcaattttaa ccctactttt tctggctcaa gcagggcaac ctgtttcttg
      301 atataaccat tatctattaa taatttgact cgacgcaaac aggacgaagg agacaaggcc
      361 acaaggtcgg ccaattcctg gttattgatt tcgcaatttg cttgcagtat ttcaagtatt
      421 tttttatcag tacgatcgag tatcatattt tatgcaatga aaatggcact tatatttctt
      481 attatagaat tatattccaa aaaaatatat taaattagaa tttaaaatca aaaaaaaggg
      541 ttttattttg ttttttgcaa tccaatttca agggaagtaa gataaaatac tttatgataa
      601 ttatttaatc ctagtggtct ttttattttc ttttccgagg gtgattatgt ctgtttcacg
      661 agttacagcg tggtttgtgt gggtcatagt atccatattt tacgcatatc aatatgttct
      721 gagagttatg cccaatatta tgctggatga tattactcag caatttcaca ttgatgccat
      781 aatttttggg caattttccg gtatttatta cattggttat tctttgatgc acttgcctat
      841 tgggattatg ctcgatcgtt ttggcccaag aaaagtgatg acaggatgca tcatgttgac
      901 cgtgataggt ttacttccca ttttgtttgc tgaaaattgg gtttatccag tgataggaag
      961 agccctgatt ggtataggtt catcggctgc cattttagga acatttaaaa taatccgcat
     1021 gacttttaag gagaaacatt ttccaaggat gctgagcttt tcagtcatga taggacttgt
     1081 tggcgcggta tatggcggcg gtcctgtcag ttacatgtgt caacttatgg gttataaaat
     1141 ggtggtggaa atatttgtag gcataggaat agtgctggct ggtattacct atctaattgt
     1201 tcccgatgca cagccttctc gccaggatag tgtgatttct aatatcagaa tggtttttgg
     1261 taatagtaaa gtgatcctat tgtgttgttt tgcgggattg atgttgggac cactggaagg
     1321 tttttctgat gtatggggct ctggcttttt aaaaatggca tacagtttga atgccaacgc
     1381 ggcaaattat cttccatcaa tgatttatat tggcatgtgt tttggcgcac ctatcttaag
     1441 tctgattgca gaaaaaacag ggtattatct tggaactatc atcgcatcag gcgtggttat
     1501 gtttctggta tttgctatgc ttgtcggagg tgttctgact attcccagta taacaataag
     1561 ttttatcctg gttggcattt gttgctctta tcaaattttg gctatttata aagtatccac
     1621 ttatgtcccc gataatcttg cagggttaac tactgcaatt gcgaatatga ttattatgat
     1681 ttttgggtat ggttttcatt ctgtaattgg ttatgtcgtc aatatgtatg gggggacaca
     1741 tgtagctcgc gcttttgttt atggagttgg ggtgattcct ttgacattgg ctgttggcgt
     1801 ggtgggtttt ttgtttctgg cctatcaaga gaaaaatttt accccggatg cttcctttgc
     1861 ctctcctgaa ttacgataat tactggcatt tctatcttgt gtggtaataa atatcaaggg
     1921 aggtatagct atcatgtata tgttgtattt tcatgttcca gaaactcatc ttgacgaagt
     1981 aaaaaatgca gtttttgcgg caggggctgg ttatgttgat aattataacc attgcgcctg
     2041 gcaaacttta ggtgaaggcc agtttatgcc tttaccaggt agccaggcgt ttattggaga
     2101 aattaatcaa ttggaaaaag tgcctgaata caaggtggaa acgatttgcg ccagagagag
     2161 aatcaaagag gtggttgcag ctttaaaaaa agctcatccc tatgaaagcc cttcttatca
     2221 agtggtacgt attgagacag agttctaaat attagagcga gaaattcagg caggtcgaat
     2281 caccagatgg ttggtcattt agtaagaatt tgtttcttca ggatacttgt tcaattgagt
     2341 attcatagat aatatctaat gattgatgaa ccaatagttc aaatccatct tcaatctaca
     2401 taagctgctt ttgaacagat agggaaatac tggtatacat gttgaaaatc cttgctctct
     2461 taagttgttt gattctaagc tcctgtctgg tcgggccaaa ttttaaagag cctaaaaaag
     2521 atgcggccaa ttattggctt acttcaagcc ctgcaataaa acaatccgct tttcaaaatg
     2581 cgaattggtg gaaagtcttt aatgatccca ctttaacctc attaatcgat aacggttatc
     2641 acaacaatct ttccttgcaa atggcaggag tacgagtttt gcaagcaaga gctcaacttg
     2701 ctcaatcagt tggggagctt tatccccagc aacaagctat ggtaggagat tataattaca
     2761 atcgaattgg gggcaatcaa ttacaagggc ttctaccgcc cgatttttta acagccagcc
     2821 tcggattttc cgcaagctgg gaacttgatt tttggggaaa ataccgaaga gcgattcaat
     2881 ctaatgatgc cctgttcctt gcctctctgg cagcttatga ttccgcattg gttactctca
     2941 cctcagatat tgccagtact tttattaaaa tccgcaccag cgaagaatta atcaaagtaa
     3001 caaaaactaa tatccaggtg caggcgatga gtcttaaaat cgcaaaggat cgttattatg
     3061 gtggtcaaac cagtttactg gatgtggaac aagcggcaac agaacttgcg gaaactcaat
     3121 caaaactgcc ccaatacgtg agtgacctcc agcaccaaaa agatattttg gctgtattac
     3181 ttggtaccac tcctgataag gtggatgact tgcttaaaaa aaataaagga ataccaaaag
     3241 ctttagcggt agtggaagtt ggaattccta aagaagtgat agcgcgaagg ccagatgttt
     3301 atcaagccag aatggaggct atagcccaat cagctgcaat tggcgctgtg aaggctaatc
     3361 tttatccttc actttcatta gtggggactt ttgtttttgc ctcaaataat attggcaatt
     3421 ctactattag tgatatattt aactggtcta atcgcaatat agtcgcaggc ccgtcactgg
     3481 catggtcttt attaaattat ggtcaaatca cgaacgcggt acgagctcaa gacgctgctt
     3541 tccagcaagc attgctgaac tatcttaatt tggtgctcaa agtgcagcag gaagttcaag
     3601 ataacattac gcgctatgta gaggggcaaa aaaccgtctc ttcacttaaa acagccaatg
     3661 ctgctgccat agaatcgaca agacttgctt tagtacgata caaggaagga gaggctaatt
     3721 acaccactgt attgaatgca gaacagcaac aattacgagt acaaacttca ctggttaatg
     3781 ccacaggaga attggctttg tctctggttg gcttataccg ctcgcttgga ggcggttggc
     3841 aaatacgcgg caagaatgat attgtttcaa atcaaataaa aagtgaaatg gctgcccgta
     3901 ctaattgggg tagtttattg aagcaagaaa atcatttgcc tccagactct aacggacaac
     3961 gaatgaaaca actatattta ccaacctggt gatgagaatg aactctcaaa ataaacaaaa
     4021 tttagttaaa accgtagcaa ttgtattttt tggtattgtg tttgtttatt tagtatatga
     4081 ccattttaca agggcccagg tagcaacccc agcaccgcca gatgtgatcg ttcaaaagcc
     4141 caaattaatg aaaatgggac agtatgttac ccagacaggt aatacagtag catttcaatc
     4201 ggtagatttg gtggcacgga ttgaaggtta tttggatgcc ataaaattta ctgacggcgc
     4261 ttttgtcaaa aaaaatcagg aattatttat tattgaaccc gagccctatc ttgagcaact
     4321 gcgtgctgct gaagccaagg tagccgctca aaaagcaaat tatgcctatg ccaaatcaga
     4381 gtatgccaga cagcaacgca tgtataaaca aaatgccacg tctcttaaca gtgttgaaaa
     4441 atggaaagca aaagttgaag aagcacaggc agaagtagat aaatcggaag ctgatgctac
     4501 aaacgccgca attacatatg gttataccca cgtttcagct cctttcgatg ggcggatggg
     4561 aagacattta gtcgatgtgg gtaatttggt tgggcacggt gccgcaacgg atttggctac
     4621 catagaacaa atcgacccta tttacgttta ttttaattta aatgaactgg atttaatcag
     4681 gattagaaat gcagcgcgca agagaggata taaaccaatg gaattggtca aaatccctgt
     4741 tgaagtggct ttgcaagatg aaaaaggatt tcctcataag gggacattgg attttgtaaa
     4801 tactggttta aacgcttcaa ctggaaccat ggagtttcgc gccttgttac ctaacaaggg
     4861 atatcccctg gtacctggtt tatttgtgca agtccgggta ccgatatctg aaccttctcc
     4921 aaaattaaca atacctgaca cctctgttca atacgatcaa attggcccct atgtccttgt
     4981 ggttgataaa gacaattatg tgcttaccaa acgcgtgaca cttggcgggc tagaaaatgg
     5041 ggtaagggct attactaaag gccttgatgc tcaagacagt gtgattgttt ccgggttgca
     5101 aaatgcgacg cccaatcatc gggttacgcc taaaactgag caacctgagt aagtatggcc
     5161 aaaaaattgt aagaatttaa gaccctttat tcagggagcg aaacaactga aatgatttcc
     5221 aaatttttta ttgaaagacc cgttctggcc aatgtcattg ccttgtttat tgtatttata
     5281 gggataatag cgattgctgt tttgcctgtg tctcaatacc ctgccattgt tcctcctact
     5341 atccaggtta ctacgaccta tcctggcgct gatgcaaaaa ctttgattaa taccgtcgct
     5401 ttgccaattg agcaacaagt caatggggtt gaggacatgc tgtacatgca atcgacaagt
     5461 actagtggag gcacctatac tttaattgtc acgtttgcta taggcacgga tctgaactat
     5521 gcgcaggttc tggtgcaaaa tcgagtacaa gcggcgatgg ctcaactccc cgaatcagta
     5581 caaaagcaag gtgttatcgt tcaacaaaaa tcgactgcta ttcttcaatt tattactttg
     5641 acttcaaaaa ataatgaata cgacggcgta tttcttgaca gctatgcgac catcaatatg
     5701 caagatgaat tatcccgttt acccggagta gggaatgtgg tggtttttgg ttcaggaagt
     5761 tatgcgatgc gtgtttggct cgatcctaaa aagatgcttg ctttttcttt gaacccaagt
     5821 gatgttttaa atgccatcag ctaccaaaac aaagaggtat cagctggaca actgggtgct
     5881 cctcctactg ttggcaaaca agcctatcaa tttacagtga atgtgcctgg acaactctct
     5941 gatcccaaag agtttgaaaa cattattatt aaaacgattg ataccccacc cgatcaaaat
     6001 tcctctgcca gtagctctgc tcaggtagta cgtattcgtg atgtagggcg agttgagttg
     6061 ggatccgaga gctataatca gctcgctaat cttaatggta aacctacagc agcaataggt
     6121 atctttcaac ttcctggtgc caatgcgttg gatgttgcgc aagaagttcg gaaagccgtg
     6181 gcgaaaatgg caaaacagtt tcctccgggt cttgaatatt ctattccatt tgataccacc
     6241 gttttcgtca aagcttcaat tgaagaagta tataaaacac tttttgaagc aggaattttg
     6301 gttttaatag tgattgttgt atttttacaa aattttcgcg cttcattagt acccgcgaca
     6361 acggttcctg tgactatcat tgggacattt tttgccatgc ttttattggg ctatagcatt
     6421 aatttgctga ctttatttgc tttggtatta gcaattggaa ttgtggttga tgatgccatt
     6481 gttattgttg agggtgtgac tcaacatatt gaaaaaggct tgtcacctaa agaatcagcc
     6541 atacttgcaa tgaaagaatt atttggcccg attattggta tcactttggt attaatggct
     6601 gtttttgtac cggccggttt tatgcctggg ttaacggggt caatgtatgc acagtttgct
     6661 ttagttattg ctgcaactgc ctttataagc gcaattaatg ccatgacgtt aaagccgact
     6721 caatgtgctt tatggttaaa ggctcccgat acgagtaaac caaagaatat attctttcag
     6781 gcctttgatc gaatttataa tcccattgaa tcggcttacg taaagtttat tgatcggctt
     6841 gtacatcgta gtggtaaagt ttgtttgatt ggaatcattt tagtggcttg tgccatttat
     6901 ggcctgacgc gtattccaac tggctttatt cccatggaag atcaaggcta tttgatgtta
     6961 agtattcagc tgccggatgg cgccagttta ggacgtactg atgaagtagt gagtagatta
     7021 gctaaaaaag catctgaagt tgggggtgta gataatgtga ttgctattga tggtatttca
     7081 ttattggata acaattctct gctttctaat gccggtgtca tctatgttat attcaaagat
     7141 tggagtgtca gaggaaaatc tgaaaattta cgagctcttt ataccaaatt caatgcaata
     7201 gccaaagaaa cccttgatgc aaaagtgttg gtggttgtgc cccctcccat tcaaggttta
     7261 gggatgtcgg ggggatttca aatgcaagtt gaattacaag acggtacttt tgattacagg
     7321 aaattacagc aagctactga tcaaatgatc aatacagggc gtcaataccc acagttgcaa
     7381 aaccttatga cgacatttcg tgcgagtgtt cctcaagttg cagcacctat taacagaacc
     7441 aaagccgaat ctttaggagt gcgcgttgct gatgcctttg atactttaca aacttattta
     7501 ggttcttctt atgtcaatct gttcacgaaa tttggtcaag tatttccagt ctatgttcag
     7561 gcggatgcat cctcgagggt tagcagcgaa gatttaagga attactatgt aagaaatcaa
     7621 tccggctcca tggtgccctt aggtactctt acagacgtgg ggcctgcggt aggaccctca
     7681 attatttctt tatataacct ttatccatca agtaacataa atggggttgc cgcaagaggc
     7741 tatagctcgg ggcagggaat ccaggttatg gagaaattgg caaaggaaca attaccccca
     7801 gggatatctt atgaatggac aagtacagct taccaggaaa aggtggctgg taacctcagt
     7861 tactttattt ttgccttgtc tttggttttg gtttatttaa ttttatctgg ccagtatgaa
     7921 aattggctta ttccatctgc gattatcctg agtgttcctt tgactttggt tggaacagta
     7981 ttagctctcg gcagtttggg tatggataat aatatgtaca ctcaaattgg cttgttactt
     8041 cttattgcat tggctacaaa aaatgctatt ttgattgttg aggtagcgcg tgaacaacgt
     8101 gaaattcata ataaatcagt tcttgaggca gcggtcattg gcgccaaaac acgttttcgt
     8161 cctattctga tgacatcttt tgcatttatc atgggggtta tgcccttggt ttttgcaacg
     8221 ggagcaggtg ccaattctcg tcgctcaatt ggtatagcgg tgagcagtgg tatgcttgcc
     8281 tcaacttgcc ttgcagttgt ttttgtgcct gttttttatg tattgttgca aacctggcag
     8341 gacaagagga aggcaaaaca ttaattaatt taacgtaagt tatggataaa gattacataa
     8401 gaaccagttg atatccagag gacgtttggg ctgaggagat gcgtaagcat tgtcttgaag
     8461 ccttggcatg aaacattaaa tcaaatacga gatttcgaga cggcataaat acctccgcaa
     8521 cctgagtggt tcgagataat aactgatttt atccatgact catgctaatt tacagctata
     8581 gagaatgctg tgagaataat tctaatcctc tcacagccaa atagtgtatt tatcttcctg
     8641 aacattcaaa cagttgatgc tatcaagtta tattgtatgc ccaagggtgt ctgtaggagg
     8701 ctcatttctt ccagtgattt cactcttacc tatttcttta taattcccgt gtacaaagtg
     8761 tctgtaggag gctcatttct tccagtgatt tcacccatcg cttccctgaa ttgttttgat
     8821 gtttgtttag tagtagggct ttggactgat ttgattggct cattaagatt tgattcctca
     8881 agcttttcag ggttggtaga ccattttata atgttatttc ttgtaaaatg ctcaggggca
     8941 attttgtcag ccagttgact gagtttttca tgaacttgct catcaatagc tttttcttct
     9001 ttgagtttgg agagtaactg cgtattgact tctttaaaca gtctgggaat atcttgttct
     9061 aggagagttt tcttatcggt tgatttatta atttccctgt attccaggct taaatagcta
     9121 gctacttttg ctgcctcttc agggttcaat gtcttaaaga attcaattgc ttcatcattg
     9181 tggcaatgag aaggcaagtc cctgacaatt tgttgaacag caggatcttg tatcttttga
     9241 aattgttctt ccatttccag agagatgatt tcatcaagtt ttgttgattt aaaggcatac
     9301 ttgtccaatt ggcaaatatc tttcaattga tctcgaaata acctgacttc cttttgcaga
     9361 gtactttgaa accaattgga ttcggcagaa atgtctctgt caattttatc taatgtttct
     9421 atagaattta aaaccttttc aagtacctgg cttttcgtta aagattcact ttgcagaaga
     9481 gtattgattt ctttggatac tctttgcaat tcttcacatt gggtcttacg agcttcattt
     9541 cttgaacctt taaaccacca ttcgcgtcct ctcttttcat tgtaaatttt tgcagtgagt
     9601 tctaatcctt cagcgatttt atttaattcc acatccaata atggcttgga ggaatatttt
     9661 gaccaggtaa ttgcgcttag attatcgtgt tgtaaattat cacgagaaag caatcttgaa
     9721 agatcttgtt tttgactctc cgggatgctg gtagcctttt ccagctcttt ttgaatggct
     9781 ggccagttac gattataagc aatgtaattt tgagtttcct tcgaaagcat ggagtaattt
     9841 ggaacacctg taggtgaggt agcaaccaaa taatccaata attcttttgg tttattctca
     9901 gccagcagtt tgacaacctc tttcttgaat tgatccagtt tttcgtaccc ctgggctact
     9961 acctcattct tgtgatgtaa gcgattggag gttccttcct ggagagcttc ctgatgactt
    10021 gcaagatcac caagaggatg tttgcctttg gtttgtcgag taaagaaatc cagaaaatga
    10081 caactgattc tgccttgatg agaaaagata ttgccttttt tatccggatc agcgacaaaa
    10141 aactcatcaa aatgtggaga ttctttaatc gtattataat gttttgtaaa tttatcagtg
    10201 atttcttgtt gttgtttgcc ggttaacgga gattttaatc ccagctcggc atggttgctg
    10261 ttaatatagt cgtatataat tggttctata tctgcacctt gcgtaaatcc ttcctttact
    10321 cttttcgcaa tttcagtagc atgaggctct ttgtcgaaaa attctccaaa gttagcatcc
    10381 gataatttgt ttgttttaca gtaaatattg gcttcggcca acaggtactg aactctgatg
    10441 gatatcttgt ccgcatcgcg gtctgatgat atttctttag caccatcata gaaagggctg
    10501 gatgcctgag ctacccagac agtggaatct actgtagcgt cgagaatagt atcaatccaa
    10561 tcaccaattt cagagtcgtt atctacaagc atcaaactat ttactaaata atcgtaattt
    10621 atttcttttc ctcttggatc tttagataaa gatagtttcg aatattgagg atcacttttt
    10681 actaactctt ttacaagttc gttttgaaga ttagtaatta gttcgtttaa tttttcatct
    10741 tttttttctc catcaattct atcttcatct ttataatgac taagaacagt gcttttaact
    10801 ttatccctaa gtgatttttt attaattgga gttggtgttt tatcttccgg gaacagtgtt
    10861 gaacgcaggc gataacctaa tccctcgtat attggaatag gagcttgtct tgagggcgtg
    10921 tcatattttg agatgtttct tttgacattg aataaagggt catcaaagcg agtgaattta
    10981 tcgttgtcat atggagaaag tctcacagcg aaagcatttt cagaggattt aatgatttcc
    11041 tttaccccag agggtaattg cggaatccct ccagtacgaa gttgcctgat atcattttgt
    11101 tcatcatatt gtttttttag tacttgaatt aattcaatgt atttttcaat ttgctgtaag
    11161 cgttctattt tctcttttaa taaatcgtca taagcatgag gtgaaatttt cttttgacta
    11221 ttgatggctt tgatgtcttc ttccaattgc cttttgtatt cactgagttg gtgttcagca
    11281 gaatatttag tcacaccact gtgcgcacta ccatagaaaa atgtgatcaa ctcaactgct
    11341 gtttgacagg tattatccag gcctatttca tctcctccta caatcggtaa aaggatatga
    11401 actttgttgt tttcatcaac atatagataa tcacaacgtt ccggctcttt gaatttgatt
    11461 acgtcaacca tgtttatcac cattttctag tgtcttgttt ataagtatat tataaagaca
    11521 atttgtttgt aaaacaacaa aaagagttgt aattaaactt tttgcttgat tacacatacg
    11581 cctcaacaca accgtgtttt tatattctca atcgcttgat tcatactact aagctatatt
    11641 gtattcctaa cagtttctct gagtgactca tttcttcctg tcatttcatt tatggtttct
    11701 ctgtattttc ttgccatttc agtaattgag ccttcagagg attttagtaa ttcaccgaga
    11761 ttggactcct caagtttttc aggattggca gaccatttcc tgatattatt ccttgtaaaa
    11821 tgctcaggag gaattttatc agctaattga cagaattttt catagacgtc ttccttaact
    11881 gcactatctt gttttaattg ggataatagt tccatattca cttctttaaa taatttagga
    11941 atatcttgtt caaggagagt tttcttatct gttgatttat tgagttcccg gtactcgagg
    12001 cttaaatagc tagctacttt agctgcttct tcaggattta atgtcataaa gaattcaatc
    12061 acttcattat tatgacaatg agaaggcaag tctcggacaa tttgttggac agcaggatct
    12121 ttaatcattt gaaattgttc ttccatttcc agagagataa tttcatcaag ttttgttgat
    12181 ttaaaggcat agttatctaa ttgacagata tccttcaatt gatctcgaaa tgattgtact
    12241 tctttttgca gagtactctt aaataaatta tattcagcag aaatgtctct atcaatttta
    12301 tccaatgctt ctatggagtt taaaactttt tcaagaactt ggctttttgt taaagattca
    12361 ctttgcagaa gagcattgat ttcttgggat actctttgca attcttcaca ttgggtctta
    12421 cgagcctcat ttcttgaacc tttaaaccat tcactttttc ttttttcatt gtaaatttta
    12481 gcagttagat ctaaaccttc ggcgatttta tttaactcca catccaataa tggcttggaa
    12541 gagtattttg accaggtaat tgcactgagg ttctcgtgtt gcagattatt acgagaaagc
    12601 aatcttaaaa gatcttgttt ttggttctct ggtatgtcag tagttttttc gagctctttt
    12661 tgaatggctg gccagttacg gttgtaagca atataatttt gagtttcctt tgagagcatg
    12721 gaataattag gaacacctgt gggggaggta gcaaccaaat aatccaataa ttcttttggt
    12781 ttactctctg ccagcagttt gacgacttct ttcttgaatt gatcaaattt ttcatatcct
    12841 tgagcgacta cctcattctt gtgatgtaac cgattggaag ttcctgcctg gagtgcttct
    12901 tgatgacctg caagatcacc aaggggatat ttgcctttgg tttgtcgagc gaagaaatcc
    12961 aggaaatggc aactcattct gccttgatgg gagaatatat tccctttttt atcaggatca
    13021 gcgacaaaaa attcatcaaa gtgaggagag ttctcaataa tgtgataacg ttgagtaaat
    13081 ttttcagtaa tttcctcttg ttgcttgctg cttaactcag atgttaatcc cagttcggca
    13141 tggtggctgt taatgtaatt gtaaataata ggctcaatct ctgctccttg aacaagtcct
    13201 tctttcactc ttttagcaac ttcagtagca tggggctctt tatcaaaaaa ttcaccgaag
    13261 ttagcatctg ataacttgtt ggttttacaa taaaaattaa tctcagctaa tagatattgg
    13321 actttaattg acattgtatt tgtatcagat tcaaatttta tttccttttg cttctcatag
    13381 aatacactaa ctttttcagt accactttca ctttcctttt ttggccaata atcagcttct
    13441 gttgtagcac caataattcc ataaacccaa tctttcgcgg tggagttgtc atctaacgta
    13501 catgctatgt tttccaaata atccaagtta agtggttggt gataggtatc ctcatccact
    13561 tgaagttcgg aatcaatttt cttgtactct tctagaacga gtttttttat atcctcaaat
    13621 ttttgaccac gatcgccatc ttttgtatta aaatctttgt caagttgcac taaaacagaa
    13681 tctacgattt tgtccttcag tgatttttta ttaaaaacaa taggagtgtc tttatctggt
    13741 ggtagaagct cagaacgcaa acgagcccct aatccatcag ttgcccgttg gtatccccca
    13801 gcttcatatt gagatctgtt tcttttgaga ctaaacagag gatcatcaaa gcgggtaaat
    13861 gagtctggtc tatccgggga aagccttaaa gcaaaggcat tttcagaaga tttaatgact
    13921 tcctttactc cagaaggtaa ttgcgggatt ccttctgtgc gtagtttgtc tatttctctt
    13981 tgctcatcaa atttctcttt gagtactttg attaaatcaa tatatttttc gatttgttcc
    14041 aggcgctctt ttttctcttt taatagatct tcataggcat ttggtgaaat ttttctttga
    14101 acaccgattg cctttatatc atcttccaga ttctttttat attcatttag gtgatgttca
    14161 gcagaatact ttgtcccacc atgcgttttt ccatagaaaa aggttaataa ctcacctgtt
    14221 gtttcacagg tattatccag tcctatttca tccccaccta caataggtaa aagaatatgg
    14281 actttattat ccttagcgat atgtaaatat tcgcatcgct ccggctcttt gaattttatt
    14341 ttgtcaacca tgttcatcac cctatgctag tgttctgact ataagtatat tataaaatga
    14401 ttataagtat ttaaaaataa gtctgattgt taaaaagatg ataatatttg tgttttttct
    14461 ttcagtataa aaaactaatc attgttcctc atatacaaga attgagctca caatttgagt
    14521 tatctaaaac aatcgaatat gagattttcg attaaatcat tgcgattttt tgagttatgc
    14581 ctaccgcaat gaattgataa ataattacac ttgttatata ataaatcctc attttgttta
    14641 tggaaggaga ccattgtgcc tattattcta gattctgatg tgttggaagt ggcggaatat
    14701 gtgtataaaa cacgcttatc tcagccttac actgaagtgg gttctgaatg ggagtataat
    14761 tacaagaatc caacagccac ttttgcaaaa ggcgatggcc ataacttgca aaggtatatt
    14821 acaattgatg gcaagcagct tcatcgccct atccatgggt tagctcatac gatgcgcaca
    14881 ttgatgtata gccaattgat gtattgctcc tcaaaaaaac aaccttcccc acacgtatgt
    14941 caagacggac gaacaatagc cgatctcagt gaacttgatc ttaaaaagat aaacattgcg
    15001 cagctatttt ttgtagccgg gcgcgaaagc gaggcttctt atggtgatgc ttatcatcgt
    15061 tatcatctgt atggggctaa acagttcgaa gaatatgccc gcaagcattt gactcatctt
    15121 ttttcagaag aagaaatccg attgtactca cgatgtatag aagacagggt aggtgacagt
    15181 tttgatggta cccctgaagg ttatataatt cacttgtcac acatgatcga tttgatgcgt
    15241 tgcaaaagcc cggttgaagt atttttgggc cactcaaaag gggtttctgg tattgtacct
    15301 acattgattc acttgtttgg caaacaagat ggcttggata ttatgcatta cgcccgaggc
    15361 ttgtttgcgg ctacaggtga ggcggtgccc tatattgatt catctgaatg gcctcatttg
    15421 ggagttgact tgagtcgggt acaacgcgca ctctctatcg ttggtgatat caatgtccct
    15481 gggcaagagg ctgattctaa aaaaacagcg caagccggtt tttctgtgga tggttgttat
    15541 tctgcgttaa ccagtgtgcc tacaccttcc tggtatgaga aagagttaaa agaaatcgat
    15601 gatgaaaaaa tcgatgttaa ggaagttgat gatagggaga tagaaaaaga acatgagata
    15661 gttgttcctt cgcaggcaac aacaccttta agtacagtaa aaactgactc ctttgtcgaa
    15721 aaacttctga agccatttat gatttggaaa aagccagagg tacaaacgac tcaaccaaca
    15781 acggagaaga ctaataaacc ttagcctgtt ttatttaaag taatagagaa acggattcac
    15841 ttagggaatt aaaacgcaat gaggtgttgt aactatgttt agttatctgg ataaattatt
    15901 agatggaata tttggatacc aaaagtcaga tactagtcag ttaagcacct caacgccacc
    15961 tactctaact actgttcctt tgaaacaaga atattttgta aaaattgggg aaagtgaaac
    16021 acaggatcta ggcttactgc cagtggttag caagcgacat aatcaatcag ttcccttgga
    16081 ggaaattcct tttgaggata ccagacttga gttgattgag ctttatattg cattgttaaa
    16141 acaatgtgtt ttagatgaaa aactgaagag tatccctgcg cagtatctta tatctcatta
    16201 tctttttata aaaaccttag ccgcaaacga gggaaataaa ggtcgaaaag atctgtatct
    16261 taatttatct caaaaagtgg ctgattatct tgaaaaaaat gaatctaaaa tatggagtat
    16321 ggctgttgaa tgcgctaaaa ccagtgagta tcccatagtt gactggataa aaaagcacca
    16381 tcttcatttt aattttataa gagcttttat actggattac aataaaaaat cattgactca
    16441 taatcaacgt gcgtttatgc agcagtttag ggattctgct gcttttttct ttcctgatca
    16501 ggtttacctt gcttggctta cccaatccta tgaaccaggc tctatcttga atcctatgta
    16561 cagggagagc agatcaacgc attattatca cgcgaacatt actgataatc tcttgttaag
    16621 aacacgcccc aaacaggtca attttggtcc tcaacatttt ttccaaaaag gaaaaggccc
    16681 cgtcaagaat acttatcgtt tcaatatcaa tgacggaaaa ttaatgcgca tacagggaag
    16741 aacattactt tttagcacga ataaaggcaa tgaagtgatt gctgtaaagg tgcaaaaaaa
    16801 aggggaaccg caatctgctt taagtaatga atttcaaatg gcagattatt tgttgaaaca
    16861 tcaacgccgt ttgaatttgc aaagtcaatt acctacccct ctcagccaat attcaattaa
    16921 tcgaacagag attctcgaaa aatgcagtaa atcacctgat tttgagaagt tcaagaattt
    16981 gataagtgat gcaaaaagcc ttgaaattta tgtttataaa gcaacacctt cttattttac
    17041 ttatctgcat gataagcagc agagctttag tcaactcact tcatcagtgc agaaaaatgt
    17101 acatgatctt ttcgtgctcc tgagagaggg catagtcttt ccttatctgg cagatatgtt
    17161 tcacacccat attgatgaga gtaaaaggag cgataagggt cgatatcaga cgttagttga
    17221 gcttttaagt gctttgcaat cacaaatggg ccgtcttgat aaatggcaaa aagcagtcga
    17281 atttgttaac ttacgagcca gcggtatcgc tgatttgggt gacaatcttc cgttgaccag
    17341 cttcttaact gtttctgact ggacgaagca ttatcatgcg gaattgctaa cgggcgttta
    17401 ccatccatcc tttctattcc tcgataaatc cagtggctcg gttcgctcct tgtttaacag
    17461 ccggcgcaaa atattcggca attatttgta tctgaatatc atcgctgagt atctattagt
    17521 tattcaattg gtaattggct gttatggcga taaagtgacc cgtaaaatga atagtaaaag
    17581 caaagctaag gtttgggagc atttagccga attgatgttt tccagttgtg ctgaagcggt
    17641 taatttgata actggtatgc ccaaatccag ggcacttgct tttttaaagc aaagagccaa
    17701 tgtaagaaaa catactcaac aaacgtcgtt ttggatgact cctgattatt cgaatctgga
    17761 taggctgagt gtgcagtctc aacaatccac tttgtacccc ggcgaatcag attatgaaat
    17821 aaacagtgat ttaatttcgg gggtcggtct gagtcttgat ggcatcaatc aggatttggg
    17881 tgattataac caggcaagcc ctttaaggga gctagagaaa ttattgtatg ctacagtgac
    17941 cttaatagag ggaacccagc aactggataa gcaatttttt caacagttgg atgaaactga
    18001 gaaaatgatt ttgagtgctg ctggagttga taaatgctat caagctgtcg caaaactcct
    18061 ggatcttgct cggcctggtt gtcaaatgca gcgaagattg gcttttactt attatgaaat
    18121 gatcaaaaga atatatccct gctcaaatcc cgcagatgtg agatttgaac ttgttgcaaa
    18181 agaagaggcg attataaaaa ttcagcgttt ttggcgagag cataaaaaag aaaaccaatc
    18241 gcttgaaaaa gggtttgatt ttgacaggaa cactagttca tcgcagtcgc ctttatgaaa
    18301 gggtcatttc aattgtaaga gatcatcccc aagatcgtta gaggatattt caacatcagg
    18361 gcactgacct gttactttta attttggaaa attaaaagta ctcataagct taatgagatt
    18421 tttcttattt ccgctaacaa ttggatttta tgctatttaa ttaagacaag attttattct
    18481 tcatccaggg aattatggtg gaagtttaaa ctcaatatca gatatgatta cgctttgaat
    18541 tgacaaaaac aagacaaaaa acgatacttt tgcttttata aagaaagcaa attttttaaa
    18601 aacggtcaat aatatcttat tgattaaata atgttcagat ggtataatgt ttaattaact
    18661 tgctggactt aacttatttc atatgaagtg tgaatcatgc ctgaagagac gagcacaaat
    18721 ttaccaaaat cactcagcca ttttcttatt caatctgatt tggattataa gaactcattg
    18781 agttcgattc ctttatttga cgttaagcga actattttct gggatgacaa ccttagaaaa
    18841 atgtttgcat caatttttta tcatttaaga gggcatttta taaatttttt gtggtacgtt
    18901 gccaattttg ctgataacga agcttcaaaa caaattatta ttcaaaatat ttatgaagaa
    18961 attggaattg gcaatcgttt ttcccatgaa aagctttatg aacgttttgc aaaagaatgc
    19021 ggtgttgata ttcatgatga aatagtgaat gaaaccaact acattccttt tgccaaagaa
    19081 tttaataaag ctcatttatc ctggttggcg caacatgatc ctaaagatca tttgtgtgct
    19141 tttgcggctt atgagcgctt ggataatctt gattatcctt ttcttgttga acttgccaaa
    19201 tcgatgaaaa tatctcaaca tggcatgacc ttctttaatg tacataccca tgttgatcat
    19261 tttgatacca ctctacaatt gattgtgccc atatgggaag aatcacctca gcgtctgata
    19321 gaatcatttc attttattca aactcatcag ttagggatgt gggaacagtt ttccaatgcc
    19381 ttatttaatt gatatttttt aacataagaa atctcgggat ttcttatgtt aaataggaat
    19441 taatcaattt tggggaattt taaagtgaaa tgagcgaatt catttttttt ccctttacag
    19501 gaaatatcac cgcctgctgc ttgcatcaga agtttacaat aggccaagcc taaaccagtt
    19561 ccttcttttc tgccagaata gaagaaatca aatatttttt catattcttt atgatttaaa
    19621 cctttagctg tatcttttat atggaggtaa ttaaaattct ctttctccca tccttgttct
    19681 agccagatag agatttcgcc tttatgggtt tcctcaatat attccagact attttttagc
    19741 aaattccaca tcatattttt aaaacccact tcatcaatcc atatggaaaa atcattttta
    19801 tcatttaaat gaatcaatga tttttgattt tctttgaaag ggtattcttc taaagacgcc
    19861 ttgagcaatt ttttgatgga ataaatcgaa aatgtactgg tatctaattt gtctcgttga
    19921 atattattta attgcatacg aattaattga ttactcattt ctatgccgcg agtaattttg
    19981 cttaaatttt ccttcaggtc cttttgaact tctggattat ttaatctttc gacaatcaat
    20041 tcctgtaatt cggcttgtaa ataaatactg gcaagcggcg ttcttaaatc atgagcgata
    20101 cttccagcca gcatgcgcat acccgataaa cgtccggcat aggtaatttc tctatctcgt
    20161 gcaaacaagg caccaatgat tattgctgca ataaacgtaa caattaaact aaaaagtgat
    20221 atggttcctg ggatatagac aaactgatta tttgataaaa tataaaaatg aaaaaaagca
    20281 agtccaacac ctgatataag taatataaga gcccccaata cactggaaac taataaaaga
    20341 aaaaagatag cggatacgca attcattagc caaagagtcg agccattgtt tatgagagtc
    20401 aaataagcaa aaaagtaagg caaacaaaag agaagaacta tataccaaaa aacaggtaga
    20461 aattttaata attgtcttgg ccaaaattga ttaaatgcca aacaggcaca gaggagggct
    20521 gcagtgatgc gtaaaataaa ttcttctgtc agttgaaaat gctccagttt ccagaaaact
    20581 ccgaaaagag ggtaattaat catcatgacg atgccaaata aagtgatttg gtggcttgca
    20641 tcttcagttt cttttaataa atattggtaa attgcagtaa atatattttt taacctttgc
    20701 atgattcctt aattatctga gtaaaaactt tgagattgtt tgggtattaa tcttattagt
    20761 cctgaacatt gaatagttag tgtaggatta gaaatacttg tataaatcat gagtatttca
    20821 atggtgaact ctgaatggat gctaccaatt agagttatga tttctacggt ttcttggact
    20881 taagttaaat aatattgctc ccaattaaaa caaataccaa ggataccata atccctgttt
    20941 tttaatacta atattacttt tccataagta aaaactcata ttattgcgct ttaatttaat
    21001 atgaattgtc ctatgcaaaa tacagcgacc tggaagctaa tgttggttgt ttttagcatc
    21061 ataatacaat accataaaga gaatatcagc ggactatatt ataaaataat tggtcgagtt
    21121 atactgcgct catcgtcttt accagccgta ctcatgctgt ctttaaatct attttcaaag
    21181 gtttttacag cgtttgcttc catcacattt cgagccggat aaggtaatgc ttctgaggct
    21241 aagtcaaggc atcgagcttt aatttctgat atggaattca aaccaaatgc attactggtc
    21301 gctaatccct tattaaagta tgcgttgtga atttctcgtt cagaatctgt ctctaataat
    21361 tgtgccaaat gaagttgtcc ccagcattct ttataacatt caatgctttc tgctttaaga
    21421 tccaggctca gataaaatcc agcaagataa aaatacgcat tagcaagcaa taagcaccct
    21481 ggcgttttgt gttcttgtat ttgagtgctt tggctagtta caaaattgta taaaaggtcg
    21541 atattttctc ttttggtgtc attcttacaa gcaccaatta agaacgtcaa aatttcctga
    21601 gccgcataaa agcaattaaa tgttgtgaag gaaagattaa gataatcata ataactactg
    21661 atttcttttt gtttttcctt taatgccgct aaatataaaa catatccaca atagaaatca
    21721 gctcttgatt gcggatccgg gtttctaaat tgaaaaatat gatctttttc taatcgcaat
    21781 ttattaaatt tatttaccca gaatggtttt aattcatcac tatccagtag cgttttaact
    21841 ttgttgtcac tagtggcttt gagcatggct aaatcaaatg gttttaacct ggatagagtc
    21901 gtaattacat tatctgtgat gccttcatca caaattaatt tttgcacatc cttcagagag
    21961 ttgggcataa gttttccttt tatcatatca aaataccagt atcgattctt taagtttatt
    22021 attttcttgt ttagcctcca agtcaccacg acgtgagccg aaaaaaccta gctcagggca
    22081 agagcgtgat aaacacagac gtggtgtttt aatatcagaa agaatgtcta aattatctat
    22141 ttgtgcttgc actggatcaa ctgtctcaaa taattcatcg acctcttctt gagcaattaa
    22201 atggccgagt cctatattct ctaataaatg atctacagca tttttgagtg taaagcgctc
    22261 ttttaataac ttttgtgctt cgactactgt ccgataatta tcggaatctt ctatattatc
    22321 acatattaat tgattttctt ctcttgcaac aatggagtct aaaatatcct ttggatctct
    22381 cgattctgat tgtcgcttat aagcagagtc tggatttttg attagccact taggtagtgg
    22441 ttcttcctca tgctgttctt taaattcctt ttcctttttt aacagactgt atttcccata
    22501 acgatattct ccaatatcaa cggcattgcc gtatttgccg cctttaataa aaatatcgtc
    22561 tgtaacagaa ccaaatttag tataaacgac tccaacgtct aatacactga taattgctga
    22621 gccattgttt ctcatgccca tgtcatccat tcgacaaacg tatttacagt tttgctttaa
    22681 atccagcaat tcatgcggct cacaccaagg atatatttcg atgtaaggat tattaagaac
    22741 agcgacttct tctttaactt ttgcatttaa ctccctaacc aaatttttcc aataatctct
    22801 gcgctgacta ttattgaatc gattatcata gggatgagtt ttttgataag ttttgaccgc
    22861 tgttttacca aatctttcaa caactatttg tttcattaaa gctttgtctt gtgggtcacc
    22921 tgccagtttt acaataggta cccgttttat tttttcatga atagaaaggg agttatcttt
    22981 gatcaattga gccagcttta acgcttcttc aaaccctttg ccaggacgaa tcgtaccaaa
    23041 agataagata ttaggggcct tggctactac atccaatgga tgatccggtt cagtacttaa
    23101 tttttgtgat gcaacggtta atcctgattt tccttttaaa tcatattttt gaaccgggta
    23161 tttatctaag tcaaaatctt ccccgacttc tcttattttt tttgcaacac cagtcggttc
    23221 agttgtattg cgcttatcac aatcaccata agtagcggca ataatagcat tttctctatc
    23281 ttctgcatta aagaattgca cactgtttgc ctggcgcatt aaatcatgag tatattgttg
    23341 taaataacgt cgagtgtagt tctgtttata ttcatggatt gtgatattga ctttaatacc
    23401 tgcctcttga aattttttga tgtcttctgg agtaataaag accccgcaat ctggtggacg
    23461 aatatgaata tcaagatgag tatcctcatc aatgggtttc attttggtaa taatgttttc
    23521 aatcacttca ggttttatcg ggtcacgtcg gtttgcctga taggctaaat gaaatccttc
    23581 acttgcggag agttttcttt tactgtaaaa accgccaagc ttttcatttg cagagaggta
    23641 aggttcttgt ttatttgcaa ttaaagtttg taattctgaa attaacttat ttctacaccc
    23701 ctctttattg gcgataatat cctgagtgtt ttccagtttg tttttaacat ttgtataagc
    23761 gtcaattgcc ttttcaatat caagagctga taaggccttg ataaaggttt gttggtcata
    23821 agtggtgcta aatccatcgt tgaaaataat attgataaat tttaatttat cgtcgtgctc
    23881 ttctgtttca tttagcgctt taaagctatt ggggtacttg tctgatacag cctgacgaat
    23941 gctgttaagc acctctaatt ccttgataat gctttcaata gattctttta atccatcaaa
    24001 atcataaaaa tttctgaaac ttttatcgtg ctctaaatca tagggtaaac tgttactatg
    24061 gaaatcgtag ctgtactctt taattccagt cagtttatct gttaattttt ccagttcttt
    24121 taaattgaca gggccagtaa attggctttt aagtgcctta aaattattga gtgctttctc
    24181 atgtctgctt aaaaatttag gattaaataa atgagcctca atatattcat ttaaggtgca
    24241 ataatcttga atattctctt tgttttcctt atataatcta gtcaaatacg ctaatttgga
    24301 tttttgtttc ttaattttac tcagctcagc gttattatct tctatagctt tttccaggtt
    24361 tacttttttg gtttctaaag agtgaattat ttcatcaact tttgactcat caaattggat
    24421 aggtttttga gagtaaatat ccattattgt aagtgattgc aggctattat catgctttac
    24481 aataattttt ggtgttctgg gactgaccgt tttgttattt acttcttgtg ctttttctgg
    24541 agatgaaaca gtatctttta atttataacc gcttattact gtattttcgt tagcaactgg
    24601 ctgtaatact attgcgcttg gtatcgtttc ttccggtata gaagtggttc ctgttgggaa
    24661 tgggatgctg taatcacctt tgtctttttt taaccattta caggggatat gttcgtaatt
    24721 ttcagcaaaa cccaattcta aagaggccgc gtattgccca tcacctgttt ttgcttcttc
    24781 atcaaaaggt gcagttaata agtatatttt agtcataaat tactttagta aaatttttgt
    24841 catggtgaat atcttacatt aagattatta aatgaacatt aagaggtata attgcaaaat
    24901 ataatcagga atagcaagat gataatatgt tgataaaatt atgagttttt ttgacttaat
    24961 tgcataaata gaacttgaaa aaagcaatta tttataattt agagcagtaa tatgggttag
    25021 atccatcaaa agtaattaag ctggtatatg tcctgtagta tggaaattgc tctctgtaaa
    25081 aggaactttc cagaatcatg accagggctt cgggagaaaa tctatgaaac cgctatccag
    25141 aaaactaggc tattgggaag agctatgtca atggcgaacc attcttattt atgaccaaca
    25201 cggtaattct catgaaatcc tgttaatcgc aggacataat attggagatg gcgtctcggt
    25261 agcctatttt ttccatactc tctttagaat ttaccataaa agtgattcaa atccatcctt
    25321 acctctctat cctcctgttg aggaactggt gcaacacaaa aaaggcaaaa atcttctctg
    25381 cctcctacat ttttgcctac tccgatttat taccagaatt atgtccctcc tggcgagcga
    25441 ataacaaaaa atcattatta tcctgttaga gtgaaacaaa ccggagtgct tctggctttt
    25501 agtaaaaatc acaacttttc tctgaatgca ttaattaatg cgctgtccct aatgacatta
    25561 acaaaagttc tcgatcaacc ttaagataaa acactacaca cccctgttaa tttaaggaaa
    25621 tggtgtatac ctgtgattcc ggtggactat tttggatgct atgtgtctgt ggtgactaca
    25681 tatcataaac aagtcagtat agaatcagat attatagagc tggccaggga ttatgatcat
    25741 cagttaaaac aagagcgatt gcccggtgac gctatatttc cagttcatca tagtaagcag
    25801 gatattattg atgctctgtc atcatgggaa ggtcataggg atcgcttttc aatgggagtg
    25861 accgtaacca atattggttt gttacctttt aatactcaat atagtcattt gaaatggaaa
    25921 aaaagctatt tttgtacatc ccggcaaaat gcagacatac ctgttttgct caatgttctt
    25981 actcttaatg agcaactcat gttttgcttt accttcacag aaccaatgtg tggctctgtg
    26041 tttcaggaga attatataaa aaattttata tccaatatgg ggaagttaac agactgttag
    26101 gtcaacgctt cttcgctatc catgtctgag tttctcgatt cagtggtcaa tcggctaaaa
    26161 tgccatgaga aaaagagggc tccaattcct gagcctatta atcccagtgg aagcgcgtca
    26221 agattccttg tcaacaaata gccggttaga atagttaaaa gagaggggcc gattaaacgt
    26281 atggtattca taaaagccat aactattcct gcattttgct taaaatccgt tagggcgatg
    26341 gagatagttg gagcggcact gacactaatt cccaagttgg caattaatga aaatgacaat
    26401 gctaacagac taaatccaaa caatttggaa gtaaccaaca tcgctgtacc accaaggaca
    26461 atgaaccagt tccccaaata gatggtggtg ataatgggta atcggtcacg aagccaaatg
    26521 cccagatagt ttccaaaaat aatatttaaa ccattcagaa taaaaataag tccataggca
    26581 agtggagagt aattcagtac ctcgatgata aggtaagagg aattaattac agtgagcatg
    26641 acagcagaaa tgccaaacat taaaataaat gatgcgcacc atagagaggg tatattgagt
    26701 atggacttat attcctggag tatttttttt accgagaacc tctcctgggg aggagtccag
    26761 aaaggggact ctagcaagga gagtttggtt tgtatcaata gaatacaggc aacgattgcc
    26821 atgactataa aattggcttg ccagccaaaa tgatgaaaga taagagcacc aattaccggg
    26881 gctataatcg gtgaaactga aacggtcatg gaaatatagg acagcacttt ggcgcgaaca
    26941 atcgtgtctt caaaatcacg tgctgcggaa taggcaatga gattggcgca gcataaagcg
    27001 aatccttgta gggcacgacc gcaaataagc atggtgatat tcggtgcaaa agcacacatt
    27061 aaactgccaa ttaaggctaa taaaaggttt ccgggaagta ggcgccggcg cccaaaatgg
    27121 tctataagtg gtccccatag aagatgcgtg ataccataaa ctagcaaata aatacttagg
    27181 gttaattgcc ccatacttgg ggtaatatta aaataattcg ttatgaaagg cagtgatggt
    27241 tgataaagat caaatgtaag caaagaaaac gcagaaagca aaccaagaaa tataatggaa
    27301 ttatgtgatt taggcatata aaaagtcatt caccaaaaat tatgcgattg taaccaaatt
    27361 cagcttgcat ttgaaaggaa atattaaagc aagttggcaa ctgccaacat gctttactct
    27421 gggacagatc gcttttcttc aaaaaaagtc accttacctg ttttaatatc atgaagaccc
    27481 gctacaatcc caatttgttt attcttaact agttcattta aaatggggct ttgctctagt
    27541 atatttttta caacatgcag agcattggct ttagccatgt catcaatcag tttggggtca
    27601 gagcaattat caatgcccgt gctttccatg ctgggtttta caacaggctg gattttattt
    27661 ataacatcag tcaaatgccc taatttaaca tctttgcatg ctcctgcaac agccccacag
    27721 gaggtgtgag ccaggacaac taccaaacgt gctcccacta cttttgttgc aaattccata
    27781 cttcctaaaa tgtcatcgtt gagaacattg ccagcaacac gtaaggtaaa caggtctgcc
    27841 aatccttgat caaaaaagaa ttcaggaaca ctgcgcgagt ccatacaatt caagatgacc
    27901 gcaaaaggat attggccata agctgattgt ttggcttgtt ttagataatc tcgtgctaaa
    27961 ggttgattac tgaggaagcg ttgattaccg tcttttaaac gttgcaatgc ctgcttggga
    28021 gtcatttgtt gttgtttagc ctgggtcatg gttttcccaa gtatgggtat ttctgttgat
    28081 gaggcataga gatggctggg gttacaaaaa atattgcata ataaagcagc gatgagcagt
    28141 ttttttggca ttggttaact ccttgttatg ttattaattt tcctaaattg ttatttgcga
    28201 ttattgatta cttcaaataa attctcaatg agcgtatctg gatattgatt gacaatgagc
    28261 gcgtttcgat cctttgagcg taaaaaacct tgattaacag catagtctaa aaattgcaaa
    28321 aggtaatcat agtaaccaga ggcattgagt acacagcaag gttttttatg gtatcctaat
    28381 tgtaccaggg taaacatttc aaaaaactca tccagagaac ctgacccacc tggcagcaga
    28441 atgaacccgt ccgacaattt acacatcaat atttttcttt catacataga attaacaaca
    28501 tgtaattccg tcaacccatc atgtgctttt tctgtgtcaa ccagagattt tggtataaca
    28561 ccaatgacat tagagccatt ttttaacatg cgattggcta ttattcccat caaaccgctt
    28621 tttgagccac cataaaccag ggtaatacca ttactgctta ggacatcggc catgttttca
    28681 gctgattgtt tatagacttc agaataacca gtgttagatc cacaaaagac agcgagtttg
    28741 tttattttag tattatggag cattttaaaa ttccagatgc aatgttttat ttataacatg
    28801 aaatgaggaa atacagttta tttttaatga gttaatattt tcattattaa aatttatagg
    28861 atttaattta attgcctcaa tgcaaaccat tcttattcac ctgcgtaaag acattgattt
    28921 ttagcatttt cctcacaggc ttgagtacag tcgccataat ttgttttttc gcacttgttg
    28981 atgcattgat tataaaattt ttgggcgcat aattcctgat ccgactgttg atcttgtatg
    29041 tctacttctg ggatatctcc agctaaaacg gaagaaaaaa ataatattga tccaataaat
    29101 acgaggaatt tgctcatgat cgcgttttcc ctggtttatt taatccaaaa tagattaaat
    29161 attatactat taaatataat aggacttagg aaaaactcca aggtcaaggc gaaaaatgtt
    29221 ttctagtgaa ggagtttata taaactgatt tattgaatta aattcgtttt tataataaaa
    29281 atagattgag attttgtgtg agcatctata gttttttctg aaaatatttc gatttaaaac
    29341 aattttttgc tttgataaag agttgctatc caagccaggg atgttttatt taagtttatc
    29401 gatataaagt catatcctct tataataagc ttaatttaaa aagcttaatt gattttgttt
    29461 caattcttct tggctggaag gttttgggat agttttttta tcctgtggcg caaaccacag
    29521 tattgcgcat ttgtctttgt atttgacata gttcgcttta tcaattttgc tcagatactt
    29581 tttattcgca tacgcattgt aattattttc agtagatgcg gctttttcaa attttttttg
    29641 gaaactattt agtctgtctg ctagaaattg agcttctttc gaattatccc attgtgatgt
    29701 caaagtttga gtaattagat tagggcaggt aaaggtttcg tcaaatgaat caaagagaat
    29761 gctccataaa ctcaatgatt cgtattgacc gtgaatttgt gagatttttt taataaattc
    29821 gcagacatta ttggcatgaa ggtttaattg tccagctttt cttgcttctt ccaaaagaaa
    29881 aaattgaatg gagtgtgccc atttgccatg ctttggccct gctccaatat cggcagataa
    29941 ataaccctgt tttagaagaa cattattgaa taaatcagcg gttaattcgc cgcataaaac
    30001 tggagtgtga ttagggaaat gaaaattgcg actggtttca aaaggtatct tttcatttga
    30061 tgtagtcccc tggttaaaat gatttttact tccccagcca tcaaaaaatt gcattaatgt
    30121 ttttttctca ggctggttaa actgataaaa ttgtgtcacc aattttttta aaccttgtaa
    30181 aaccagctct tcattacaaa agaattggca tatcgacttg gcatcttctt tcaaggattt
    30241 ttctaatgtt tcgtatccaa aagaatccag gtgttcctca taatcattgg tatctgtctc
    30301 tccatttcga ataagttcta atgtcatagt gattcctcca gctgctgtat gagttattgt
    30361 caaataccga tttgagagtt atttgaggag cttaattcct tttttgaact aaaggattat
    30421 caattaatca taaactgata aaaaaagaaa ggagtatttt gttgctatta aattgcggtc
    30481 tgctcatttc gtgataaaat ataagtccag cattaggggt gccaattgat aagttggctg
    30541 agaggatcac ccaacccttt gaacctgatc tcattagtct gagcggagga aaatgtgtca
    30601 attgttattt tttctggaat agttgctttt attacctcgt ttattggact tttaccgcaa
    30661 atcgttaaat ccttaaaaac caaatcaact gaagatcttt ccatgatgat gttagtgaat
    30721 tatctagttt gttcgctggc ctggatcatt tatggaagca gcacaaactc attttttgta
    30781 acgagttcca atgtagtggg tttgatagtg agttttctgc tgattttatt aaaaagacgt
    30841 tatgatgctc gatgtaatta actttaaaga ctatcgttct attttatgtc ttaatggtga
    30901 tttgcccgat ccttcctttt ttcataaaag aaagctgccg gtcattgcag ctgatggtgc
    30961 cgctaatgtg ttatgtaacc ttggtgtctt tcctgactta attactggag atctggattc
    31021 tattcagcct gctttgttgg aaaaccattc ttttcttcat ttaccagatc aagggagtac
    31081 ggattatcaa aaggctatgc attatttaca aactcatgat ctattgcctg ctatcgttgt
    31141 agggataaat ggtgggtatt tggatcatat tctgaataac atcaacattt ttatggatac
    31201 aaactgcctt ttatattcgc ctcccctcaa aggttttgtt gtcaatgaaa agttgagggt
    31261 taattttatt ttacctgtcc agactaaaat ctcgttaata ggtatccctg aggttgtcct
    31321 gtcttctgag ggtttgaagt gggagttaaa gcactcacac ctttcttttc ctggaaaaaa
    31381 ttcctgtttc aatcgaacac aaacagcaga aattagtttg gaggttcacc agggcgccgc
    31441 ccttgttttg atttatgaag atgctattga ggatgctgga gtttcctctt tataaggaat
    31501 aaagacttac gccctcccca atctctttgt taaaaaatat ttttgcctcg gccttgtggt
    31561 ttttcgtaag tcctgtttat attagagtac tgcttgcata acctgttcct tccattgctg
    31621 taatcgctat caggtggatt accaagttaa tgcttacatg ttaaaatcca tttgcctcca
    31681 tgcttcgtac aatatgattg ctacagcatt tgataagttg agactacgat tattttctct
    31741 catgggaatc cgtattgttg agtattggtt cagtatttgt tgaggtaagc cgcaagattc
    31801 gggtccaaat aataagatgt catctgcctt ataatcgacg tcactatact tcacttttcc
    31861 ttttgcgcta cagcaaaata atcgtcgttg tgaatgagac tgcaagaatg tttgccaatt
    31921 ctcataatgc aatattgagg cccattgatg atagtcaaga ccggctcgtc gcatttgttt
    31981 atcttccaaa gtaaagccta atggatgaat caaatgcagt tgagctccag tattggcgca
    32041 gagtcgaata atattgcccg tgttcggtgg aatttctggt tgataaagcg caatatggat
    32101 catgtgattt tccagtaaca ttctggctgc cattttaatt gatttattca aagccaggaa
    32161 tagagtggca ggatgcaatt ttactacata ctttaaaaat caagcaggga gttgatgtgt
    32221 ctaatttttt actcaacagg ctgtctgaat tcaacatagc tactacaata ttagtatgcc
    32281 atatgaaagt ttttgatgtc tgttgggtat gatcattgtt gaatagttgt tgagtattaa
    32341 aaattcaaat taaactgggc cgggaagtta aggattcatc atggataaga atcataatac
    32401 aaaaaaaatc ttgtttaaaa atcgttttgt catattagga tttggttgcg ttggtcaggc
    32461 attaatgcct ttaatctttg agaaatttga tatcaaacca tcgcaagtga caatcattgc
    32521 cgcagaaggg acaaaggtcg atgttgctca acaatacgga gtttcattca agttacaaca
    32581 aattactccc caaaattatc ttgaagtgat tggtagtact ttggaagaaa acgatttttt
    32641 aattgatgtg tcaatcggta tatcaagtct tgctttgatt atactatgta atcaaaaagg
    32701 agcattgtat attaatgctg caactgagcc gtggaaagaa gaatttgtta tggagaagat
    32761 ggcgcttaat cgccgaacca attattcttt gcgtgaagaa gtccttcgct taaaggataa
    32821 aactcaaaaa acagccttaa ttactcacgg ggctaatccg ggtcttgttt cacattttat
    32881 taaagaggcc ttgttgaata ttgcaaaaga taatggcctt actattaaca gacctaaaaa
    32941 tgcagcagaa tgggctaatc tcgccatgac tttgggaatt aaagtaatcc atgttgcaga
    33001 gcaagactct caagttacct atcctccaaa atcaccaggc gagttcgtta atacctggtc
    33061 cgctaatggt ctgattttgg aaggactgca acctgctgaa atcgggtggg ggacacatga
    33121 agctcattgg ccccatgacg cttattctca tagtaatgga ccacaatgcg cgatctatct
    33181 gtctcgccct agtgccggtg taatggtgcg ttcatggacg ccaacactgg gtgcttttca
    33241 cggtttttta attactcatg cagaaactat ttcacttacc aattttttaa cattaaaaaa
    33301 tggatctgaa ttattgtata ggccaacagt tcactacgcc tataatcctt gtccagatgc
    33361 caggctctcc attttcgaat taaaaagtaa tgaatggaaa ccacaaaaca agaatcgcct
    33421 gattttaaac gaaatcattg atggatgtga tgaattgggt gtgttgttga tgggtaatca
    33481 aagaggggcg tattggtatg gttcaactct ttctatccag gaagcaaggc aaattgcccc
    33541 ttataataat gcaaccagtt tacaagttgt cgccagtatg atttcaggaa taatctgggc
    33601 aatagagcat cctgatgagg gtatcgtaga accagaggaa gttgatcacc aatatattat
    33661 tgatattgcc aaaccgtatc ttggaaaagt cggcggctat tacacggatt ggaccccttt
    33721 aaaaaataga ggggaacttt atcctgaaga agtggactta tctgatccct ggcaattttt
    33781 caatataagg gttaattaaa aggagtaacg tattgtaata attagtatta atcaatgaac
    33841 tatgcattat cataccagtc caatcaattg gactggtgac tccgtagcat tgattatttt
    33901 ccatggtaaa gcaagggtcc aggcgccaat gggtggcagg gaatgtgcat tggcaacgtt
    33961 ttcaataata tatttacctg cttgtaataa cagttgatgc acaggataac ctgagtctgc
    34021 ggtatcagga gaaagcgtgt ctatgccaat gccagcaata tttctttcaa gcaatagttg
    34081 ggctgcgtga gcactaatgc ttggaaaaac caaattgttg cgatatttgt caggattagg
    34141 ccaatgttta tcccaaccag tataaaaaag cacaaacaca ttctttttta acctcccata
    34201 tctggcttcg aaatccagaa tatcctgttt tgtaatttga taatgttcat ccgtttgatg
    34261 actaacatta atcagcacac tttgtgtgag caggtaatta cggtgcagat cgagatcagt
    34321 aatagaggcg gcccctgtaa tacaatgggc cggagcatcc atatgggttc caatccccaa
    34381 gggtgagtgt atttgctgaa ctctgaattt gatttccgat gtacagtctt cataatcaat
    34441 aaaattacta tgggtaaatc cacaatccag atcccaacca ggggattcag gtgaaacaga
    34501 gtgtgttaaa tcgattattt gaaaaggaaa tttcatagtc atttgagaga gcgatggatt
    34561 atttcaatta aattatcata aaaagaatca ggatcaatga aactaatttt taatttaggt
    34621 tttgcaaaag agtgtcagtt tatcttttta ttataataat gatcattcaa gctaataatg
    34681 aacaatttta ttcaaatttt acaataatta tggattagta ttgaaatatc cattggaatt
    34741 attttaggcg ctgtcatagg aagtttaaat tcacccgggt attgggtcag tgacaggcgc
    34801 tctcataggc ataattctta tgttattaac taccatttgg gaaagtagag ccaaaaaata
    34861 agaatggggc tgactccatc gctttggagc ctgaccctga ctgaattaat tattttgctt
    34921 tttcaatttt aattgttttt acattttcct tggcttcagt ttttttaggt attgtgatcc
    34981 ataacatacc ctttttaaaa gaggcagtcg ctttatccac atccgcagat agaggtaagg
    35041 aaatggtgcg ttcatatcgc ccataactaa tttcacgact gatataattt ttattttcat
    35101 cttttttaga agtggttttc tctccttcaa tggttaatct gttttcacaa aaagatactt
    35161 taatatcttc ttcacccata ccaggcattt ctacttcaat tttgaaatta tctttatctt
    35221 caacgatatc caaagacgga gtaagagaaa tatgctcgaa ttgctcaaga ttggcacttc
    35281 tttgagggtc aaataaatta taaaaatcac tcatagcatg attgacctct tgctgaagtc
    35341 ttaaaaacgg actcatcaga agatcagttg ttttacctgg ttgatgtttt cttaagctgc
    35401 ccatgatgtc acctccttat tccataaatt gacgtatcag ttagttgtta aaaacaccga
    35461 aagtaatatt caccagctta tcaatgtaaa tatagttgct agataaaata ttttcaattg
    35521 ttttaaattc atttcacaat gactttatat tgttattttt gtatttattt tattctttct
    35581 aaatgttgct taaaccaatt ttgagcgagg cgagttactt ccttgatgat ttgaatttgt
    35641 tgtaatagct tttgataatt atagccttat ttcataacaa taaccccggc tcctctatac
    35701 ctgccatgtc ttaaatcatc cagtgctcga ttaacttcgg taaggggata ggtttgaact
    35761 tttgtcttaa ctgggatttt aggggctaga gacaggaact cttctccatc tttgcgagtt
    35821 agattggcaa ttgaacatac agtgcgttca ccccatagta tttcataagg gaagctggga
    35881 atatccgaca tatgtatacc agcacaaacc acagtacctc cttttacagt atggctaaga
    35941 gcctgaggca ctaatgcacc aacgggtgca aaaatgattg ctgcatcaag aagatgaggt
    36001 ggtgattgcg tagaatcccc ggcccagaca gcacctaatc ggtatgcaaa tctttgagtt
    36061 tcctgatcgc ctgggcttgt aaatgcataa atttttattc ctttttggca agccacttga
    36121 atcagtatat gtgcggccgc tccaaatcca tatagaccta aatgagttgc gtcaccagcc
    36181 tttagcaagg cacgatatcc aattaaacca gcacaaaata atggtgctgc ctgatgatcc
    36241 gggtatccat cgggaatagg aaagcaaaaa tggtaatttg ctacacagaa ctcagcgaaa
    36301 ccaccatcta tttgataccc ggtaaagcag gcttgatcac acaaattttc tcggccagag
    36361 gtgcaaaaat tacagatccc gcaacttcca cctagccaag ggactccaac tcgctgtcca
    36421 attgcaaatt tcttaacagc ggatccaacc tgctcaacag ttcctacaat ttggtggcct
    36481 ggaataaggg gagtcttggg atttttcaac tcaccatcga caacatgcaa gtcagttcta
    36541 caaatcccac aagctgatac tttaattaaa agttcatttt catttggatt tggtttattg
    36601 acttcctgat attgcaattc acatccaggc ttttccatga tcatggcatg catgggtatt
    36661 ggtcctgttt ttatcctcta tcttacaatt atagtaggat gggttatact tacttaatgc
    36721 attcaaaaag taacaatatt tgacatcaat gctgatgaat gaatagcttg cctggaggcc
    36781 attaatcggg gtcgttgaca ggcagcaaaa aatttaaaaa cagtagtgat cttgataatt
    36841 aatttggaaa aagcgtttgt ttttcggaat ttattttgtg gattgcaata tttttccaat
    36901 aggcaactca tattatgaac gctatacgtg ctttttttgc tatttcacta ccaccagcga
    36961 tccaggaaaa tatccggagt aatttggagc cattgaggca aatggcacca gttcactcca
    37021 taaggtggac aaagccaatg catttgcata tcaccttgca atttttacag agactggaaa
    37081 caaaacatat tcaggtcttg attgaaagag taagtaatga acttaaccat ataaagcaat
    37141 ttgatttgga attcggtgtt ttagagtggt tcccttctcc caaaaatcca agacttattt
    37201 cattaactgt agatcctcaa gacagactta gggattggtc tcgaatgatt ggtcaggtga
    37261 tggacagttt aaagtatccc ttagaaaaac gtccttttag agggcatttg actgtaggta
    37321 gagtggtgaa tttcagccca aaacttcagc agttgttgca aataaaattt cctatcattc
    37381 caccagttcc gattaataaa ttttatttaa tcgaaagtag gcccgataaa gaaggaagta
    37441 attattttcc cttggcacaa tttgatttaa cccggtaaaa gagaatggct gatttgccaa
    37501 ttggaacgat tctttgcctc tgtgtccaac ttattatgct caagtgtctt tattattggc
    37561 ataggatgaa tttaagccaa cctatgccaa aaagagaaga ttatccataa actaatgttt
    37621 cggagtgagt ggaggaaatc gctttttcca acgcgtccaa ttttttcccg ctgttagttt
    37681 ggctgaaaaa ggtataattg ccagtagttg ctttgttaat caaaatagcg gcgagataac
    37741 cgattccaag caagaatacc cctaaagtaa cattggctaa caggtacttc cattcttcac
    37801 gatgttgatt tagcgtttcc ttcgagtttt caatatgccg tttggcattt attttgaatg
    37861 agtttaaggc acttttttca tcggactcat tattgtcgag ataatttttt atctctaaac
    37921 gaagttttgt tgcgagtgtc tttgccgctg ttgatgcttc tgtttccctt ctttcctcca
    37981 attctttggc cttttgttca atttgctgta tttgcaaata aataggagac actctttcca
    38041 ttcgcgcaaa gtaaattccc tttttctgtt gtaaagcttg ctgatagcgt ttttcagcct
    38101 ctttactttg agcaattata gattctaatt gatttgtatt taattgttgt tgtttaattt
    38161 ctttttgtag acgatcaatt tcctcttgca gatgtttttg ggctaattgc atttcctcat
    38221 tttgtttgag cgaagaatct ttcttaatga gttcttctct caattttcta atggtttgtt
    38281 ctttttcatt tgaagattga tgcaattgtt ccaattgaac atttaattca ttcaattttt
    38341 tcttaagatc ttcaactgta ttcttattat gtaaattttc cttgctcaaa ctttgatttt
    38401 gttttccaag ctcttgattt gcagatacaa gttcctgaat ttttattttc aattcttcca
    38461 attgtctttt ttgatcttct ataatctctt gaagagcaat aatttgtttg gattgctggc
    38521 ttatcaaaga ggagcctttt tctttttcct gaattacctc tccaagttgc tgtaattgca
    38581 attttaaatt gcttacttgc tctctaagtg aagccacagt tcgttttgct gaagctttac
    38641 ctttctcact ttgctcaact cgtttttctt tttcttgtaa ttgcttctca aattctctaa
    38701 tttgtaattg tttctcttca agttgttcgc taagagagtg gacttgtttt gttaatttaa
    38761 gttgattttc ttcgtttttt cgagttaatt catacatctc ttttcgaagt gattcaagag
    38821 cagaatcagc taatgatttt tctttagtaa ttctttcaat agtttcttcc tggtgtgtgt
    38881 tgcttttctt ttgtttttgt aactcctctt ttaagccctc tacaactaat gtttgctctt
    38941 gaatttgttt tcttaaagat tttaactgca attgtaa
//
LOCUS       DACTJE010000010        38459 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.010, whole genome shotgun sequence.
ACCESSION   DACTJE010000010 DACTJE010000000
VERSION     DACTJE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 38459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38459
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.010"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            73..1878
                     /locus_tag="I9760_000370"
     CDS             73..1878
                     /locus_tag="I9760_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter"
                     /protein_id="HAT5042165.1"
                     /translation="MNGHLLLNVFIFLAAASIMVPLASRFRLGSVIGYLTVGMLIGPF
                     GLKLIGNAEQIMHFAEFGVVMMLFLIGLELEPIMLWKLRKLIVGLGGLQVLLTTIALS
                     SAAVMMGYNLRASVAVSMALALSSTALVLQMLQEKNLLKTAEGETSFAVLLFQDIAVI
                     PILVILPLLGNHHGINVAINPHTAASIAQLPKWLHAIVVAGIIGSIVLAGHYLSRYLF
                     LIIAKTNIREVFTAFSLSLVVGITLLMETIGVSPALGAFIAGMVLANSQYKRTVEADI
                     QPFKGLLLGLFFISVGMGINFNLLFSQMAVIIPIVLGLITIKALILFTLGKIFQLTKI
                     QALGFALGLSQGGEFAFVLFQYSSTLKVISPEINSFFTLVVALSMLATPFLMLLYHRF
                     MIPKLISKLPEREYDSIHEKNNIIIAGYGRFGQIIGRFLSGENIKVTVLEKNPEQVEL
                     LRKFGYTGYFGDANRLDLLKSAGAAHAQLLIVTVGNPDTNLEIVKLAKEEFPQLKIFA
                     RARNRRHAYELHKAGVHYFKRELFDSSLTMTKEILKFLGYKPEEIEKKANAFQKHDEE
                     TLIKSFDFFEEEANLINFSRQAKGELERILQNNNP"
     gene            1920..2834
                     /locus_tag="I9760_000371"
     CDS             1920..2834
                     /locus_tag="I9760_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAT5042166.1"
                     /translation="MAFIMIKQLQQALSDILLGKEEVIRLGITCLLANGHLLLEDNPG
                     MGKTTFSHALAQLTGMQYRRIQFTSDLLPSDLLGVSIYDHQRNVFQFHKGPIFSQLIL
                     ADEINRATPKTQSALLEAMEERQVTTDIGTEALPQPFFVIATQNPSCHTGTYPLPESQ
                     LDRFLMRLVIGYPPPDAERMLLKSSNLNAQLKELIPIINPQQLQILQRESQKVLVSEL
                     VLDYVQSLLDASRNQGWFNHGLSPRAGMGLVHAAQAFARTDDRDFVRPDDIQAVMPAV
                     VNHRLIIKKSQTQLSAAELLMSEIEVPV"
     gene            2831..3781
                     /locus_tag="I9760_000372"
     CDS             2831..3781
                     /locus_tag="I9760_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042167.1"
                     /translation="MIRQIKTKVMAYWEEWIAKRISKTNPQILGSQNIYIIPSGFGWA
                     YGVVVLSLFSGAINYQVSTVFLMSFILAIVGFISAWEAHANLKGLSIKLVSVEDTYEG
                     TPAQVSLLIQSNNRMHFGLDFRLNNQSVTRLEKIPPQGLRFIFPLATTRRGCYTLPKI
                     TISSFFPFGLFQVWGYANFDSNYYVYPQPVNPGFWPIPLYHQNKRKTDSHGDNEYYDL
                     KQVDNPWIQPNLIAWKIAAKGQGWYLKTMDRAEEMYWLFRLNDLPIETLEEKLKHLSY
                     WLVTAESQGQFYKLELGNDPDQFSHGEEHLRYCLRQLAVY"
     gene            3781..5769
                     /locus_tag="I9760_000373"
     CDS             3781..5769
                     /locus_tag="I9760_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing transglutaminase
                     family protein"
                     /protein_id="HAT5042168.1"
                     /translation="MDFFSKQHTFLITTRYALLVMLICYFPHFLTCPLWLTALILTTL
                     CYKLISDYFAYPLLDGKIRFIVVIICLFLLKFQYGSIISSGFFIGFLLAFIGLKTIEI
                     HNDRDIRILILCNFYLIFAALIVVQELWIIPYLLIAILANFTLMLKLTAPQASLRQIN
                     GHSMKLLLIATPLTVFLFYFFPRIANPLWQVPSLEKNHTGFSEWMEPGSVANLLNDDR
                     TALRIIFKRKAILNGYWRGLTLSRYNGISWNPAWHDSSTFSSLKELTTSDDGDYEVIM
                     EPHQKKWLFYLGYPNAAYPQLLFSPNFGLVSHNKDMIAQRFAYALRISSSPHSSLTQK
                     DWKLNTQLPKYSNPRITFWAKKQFAEVNYDPQAFIQFIQNYIKKESFWYTLTPPKLDN
                     ENDQMDYFWFDSRKGFCEHYASAVTVVLRAVGIPARVIVGYQGGEWNPLAKYLTIKQY
                     DAHAWVEYWQKDFGWKQLDPTAFISTSRIDPSVLAMRAARTDQQLIIDPLNLNWIQKI
                     KLYMESTRFFIERWLLFYNQDAQYKLLEQIGLKHWNAAMLLQIAILSLVLFIILLGAC
                     YQWWQKRTLDPLLREYHLLQKEFCRFNIATQPPATLNKQCKILINKIPNLEETITTFL
                     SHYEKLRLRHRITKTKENKKQTLLLFKNLRRILKQVKI"
     gene            5851..7038
                     /locus_tag="I9760_000374"
     CDS             5851..7038
                     /locus_tag="I9760_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAT5042169.1"
                     /translation="MVHFQKMTVQTFLKDYWQKKPLIIRQALPDFTNPLTPDELAGLA
                     LEEEIESRLVYETPDQSPQWNLKRGPFKESDLIGLPKTHWTLLVQGVDRIVPDVYELL
                     EHFNFIPQWRVDDVMISYATLHGSVGPHYDNYDVFLYQAKGRRLWSLTSKKCHTNNFI
                     KGLELRIMKEFEVEEQFILEEGDMLYLPPHIGHYGIAQSEECMTYSFGYRSYQGQELW
                     DSLGDYLSEHGSFKSLYQDPDWSTLKNTSEITPKAWDNARQLLRQVLENDQLMQSWFG
                     CFATSLDQSAEQYLPPPLEEDELLGLDEFIKELANYQEIVRDASCRFAYIMSDQESQC
                     HFYVNGKEWDSRGVSTNLLSFVANNRFLSLKELKPYLNHKANQLFLYELWKLQWLQIS
                     HDQ"
     gene            7146..7472
                     /locus_tag="I9760_000375"
     CDS             7146..7472
                     /locus_tag="I9760_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042170.1"
                     /translation="MARFSRISLSTVLLSLICFNIFASDTIMITVNTKEKNAIAIGYS
                     VNGNQYGGLGSSYSGHGPKNKEYQFGYKKDSIFGKDISCGSMTLNQDSKVTLVNNNNQ
                     CISVLG"
     gene            7919..8821
                     /locus_tag="I9760_000376"
     CDS             7919..8821
                     /locus_tag="I9760_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5042171.1"
                     /translation="MKKNSLLAISEEGFHHIAYTEWGIFEPELPTVICVHGYTRNSRD
                     FDELANYLSSKGRHVFCPDIAGRGDSSWFKNPRYYNFTQYVKDMTVLMARTNAHQIDW
                     IGTSMGGIIGMILAAMPNSPINKLVLNDIGPQIPIHGLRRIAKYAGKAPDFKSLEEAK
                     QHFKASYADFGITNEKQWDIFTKNSVEQRGPNLYVTKMDPAIKKSKSILQIISEFFRH
                     PHKALEGIFYDINLWSIWKQIKCPVIVIHGVNSDILTSEIITQMKKTHALTEVYEVEN
                     AGHAPALLDLTVHQRIEDWLATHQ"
     gene            complement(8851..9753)
                     /locus_tag="I9760_000377"
     CDS             complement(8851..9753)
                     /locus_tag="I9760_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAT5042172.1"
                     /translation="MNSQEQRGSFYAILSGFLYGFIGYFGISAIHGDLSASNMLFWRF
                     LISSIFILIVLIPQLKNINDTYKSMFTAFLSGVLYYGLSTLLYFYASLYIGSGLSMVI
                     FFTYPVIIMVFNYFFYSQTIPKIYYLAISVILIGMALLIDVNEIAFDLWGIFLGLASA
                     FFYACYIVSSKNNEISPNVSTLMVCLGCMTTSLIVACLDHTFKVPTSFPVWLNLFGIG
                     LVATVIPILLMLHSLKYISSEKASILSVLEPVFVVIFGVVLLGEELKPWHALGIVLVL
                     AGALITLFSHRINLNQWLGAIYKS"
     gene            10011..11150
                     /locus_tag="I9760_000378"
     CDS             10011..11150
                     /locus_tag="I9760_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HAT5042173.1"
                     /translation="MFRVLIFLSCLVILTPNTIADTNPCEKHSCIAVIDAGSTGSRLH
                     IYSYDTDDTNTPIHIEEIWNKKIKPGFASIQPNSVTIDAYLTMLLADAPIHNIPVYFY
                     ATAGMRLLPQSQQKKYYDELEYWFRQQSQWQLAEAKTITGNDEALFDWLAVNYKLDTL
                     KSVQNKSVGVMDMGGASVQIVFPMPKNAEISKHNQVELNIYGQNINLYVHSFLGLGQT
                     EMSHQFLNSPSCFANDYPLPDGESGQGNAPSCKEEVTSLMNSVHKVNQQIQPLLALNP
                     VNEWYSIGGISNLASSQLFHFENSELTNQSLLQQGDNQICHQQWDILNGQYPDDEYLY
                     QYCLLSSYYYALMVDGYGINPNQTIHYIPPEQNLDWTIGVVLHRA"
     gene            complement(11155..11688)
                     /locus_tag="I9760_000379"
     CDS             complement(11155..11688)
                     /locus_tag="I9760_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAT5042174.1"
                     /translation="MHHVEDLISIFNSCFEKDYNTRLVRGRDEPIYLPADDKRPYHAL
                     FFAHGFFSSALHECAHWLIAGEERRKLVDFGYWYAPDGRNEEQQTLFQRVEVKPQALE
                     WILSMAAGHKFRVSIDNLNGTEVDTEAFKQSIYNQVFHYCTQGLPQRALHFRNALCAF
                     YRQPSPLRIDDFSIDEL"
     gene            complement(11699..13504)
                     /locus_tag="I9760_000380"
     CDS             complement(11699..13504)
                     /locus_tag="I9760_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1907 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042175.1"
                     /translation="MVRECTSFKGGLNLNFIENAEFESRKNIPPEEQRAVLIRNTLRL
                     LMMGWNTSWTELFSWETFKAIYLQRDPLLLKEFRLAFQKGFDALFTQLSGRQFNSQQN
                     EQIQLYLSNCLGYLPLFDLNPYESIKVPQRINDQWVMVDYYIKPIELTNHRGKNDLIL
                     RDDDRVFAYGLEPIDNPNAQSHLIFMGTTYPAGQGFISQLNTDLKGFETVGYSLYKNG
                     RDRIKDWILKQNSKIHVCGISLGGSLSLLLAIDMGKYLSRVDALNPAGLHDTDNKVKY
                     DKWYKIKKKPLVVVQQQGNDPVSFFGVWKKEWNILHVKPPKDKQGPNLFCDHFLNYAG
                     FAETEFTYVSAEQQNAKRKARNFWLYSLGRGVLYYFGLAPYTYLIRPVIHYIAEKWPL
                     IVFGIIPLLTIGTLIGLSTAGIIPAIVSIGTLAVFAISSGIFYLSFGLSNNEEDKNTW
                     AGMFDFILKGKEKVPDENELKLAKLHHPSTPRNPEMDIYKNETAIDLTYKQINTYYQV
                     TRCLLKQKEFLPLKDKPEKRFGGLSKKEALLASMNSASHDEVISLQIPKAKAVHIKHS
                     LTLAAKIGMENEIELKSALKKEYNQYQIGKHQRLC"
     gene            complement(13821..14426)
                     /gene="gstA"
                     /locus_tag="I9760_000381"
     CDS             complement(13821..14426)
                     /gene="gstA"
                     /locus_tag="I9760_000381"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAT5042176.1"
                     /translation="MKLYYCRGACSLVVRIILNDLGLNFQDEEVDLKVKKTAGGDDFF
                     KINPKGAVPTLVLDNGDILTENQVILQYLADTTPGQKLLAAVGESKRYHTLEWLNYIS
                     TELHKSLGMFFNPTVSEEIKSKILTPLVMTRFAYTNTRLEKGSYLMGNEFSLPDAYLF
                     VMVSWAKYFKLDLSQFEHLTKFMDLMLARSSVAKSLQQEKL"
     gene            complement(14622..15617)
                     /locus_tag="I9760_000382"
     CDS             complement(14622..15617)
                     /locus_tag="I9760_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042177.1"
                     /translation="MAICINKETDHFFISIGKINQHSFIMLGVYDDFQVSHLLCRVGK
                     IFDLPNQTKGIKRCLSIYSALSGAIFASSKAKIEDEGITRKRKGSAPISYQAYDISYE
                     QYCEFVHYLESIQTESNQFECFKPLVQNGNAVYFSQTSSRVFATGSHWKELNEEIHEI
                     NTGNTCRHSAIKLIEAVTKTSVPSSISSCFFINLPYKTQLDYGKPSQNIPFYVLPPPP
                     PSIHPGFNKEKRLIAKKLYHRIEQLPVLEPNSPMTKRKFNSLKNLYLQIIGSQKNQSI
                     DELLFGIQQWKEKNRVDLQTLRRTYFWDSFIVRESATMKLINEIEKDLKCAKCPY"
     gene            complement(15658..16920)
                     /locus_tag="I9760_000383"
     CDS             complement(15658..16920)
                     /locus_tag="I9760_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAT5042178.1"
                     /translation="MKINNLVGFLLAFCLTSLSYNISAAESPVVSHPKIQKEMSKYFK
                     VYHNKEKFTAIAVSVFIPQEKNKDALDVATFVKGTMGYPPLSQEITPLNLFDIGSITK
                     SFTSLILLQLQTEGKLSLDDPLGKWLPQYKEWKDVKLRQLLNMTSGIPNYSEDPEFAK
                     KMEDNFAAIWGDEELLGYAHPEKPIETDRANRYEYSNSNYILAGMVIEKVTQDTFENQ
                     LKQRIINTNNGLNNSFYPAGPHGPEVEKSIRDRRVHGYFYDENNKKLIDTISNDLSWA
                     GTAGAVIANTEDVGRWVQLLYHGLLIEPAFRDEALSELESVVSIKTGLPIPSVTEDEP
                     YGFGLGVGFYYDKNSRQRFWTYQGSTLGYRVMYLWNPCNNVTVVVALNGKGGSGNEKS
                     KIGNHIHELCLNIHKVIVKNHPHLQCAS"
     gene            complement(17167..18579)
                     /gene="gltX"
                     /locus_tag="I9760_000384"
     CDS             complement(17167..18579)
                     /gene="gltX"
                     /locus_tag="I9760_000384"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAT5042179.1"
                     /translation="MTVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQFILRIEDT
                     DRERSTQESVQAILDGMAWLGLNFDEGPYYQTERYARYQQVAQQLLEEGKAYRCQCSK
                     ERLEALREAQLAAKEKPRYDGHCRNQSLPDSGIPYVIRFRNPDAGIVSFHDEVYGDIH
                     VDNSELDDLILVRSDGHPTYNFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKALDA
                     PVPVFAHLPMILGEDGKRLSKRHGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIF
                     SVQEMITSFDLKNVSRGVSSFNYDKLYWLNQHYQKSDSPESVANALQWHFEQAGIDLN
                     QGPDLKDLVAVQAERCKSLAEMCQISQYFYTDIIEYNEDAVKKHLRPVVLEPLMVLHE
                     RLKALDEWKNDKIQECINDVSLQFDLNLGKIAQPLRVAVTGSGTSPSIDMTLALLGKN
                     KSIKRLEDALEKIRARASVV"
     gene            18678..21410
                     /locus_tag="I9760_000385"
     CDS             18678..21410
                     /locus_tag="I9760_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT5042180.1"
                     /translation="MLKSIGIKYQLRITTLIPAFLVALLFAFFYNGLFGKDLKQHMSR
                     LGEAYIRQLLPAAQYAMLRNDYRTLQGLINASTINPEVKALAFYNADGRLIAYRGGKH
                     SIHKPFNPPDYTGDYIESKQINPFTINFIAPITIPKFNLYSSTEFKELSTPKIFQADD
                     ILGWLSIDIDTQSLLIKRYQMLIVTIFITLFGLLMGLTIHYFLSKRIYMPIARLRRSM
                     KQILSNEFETEIRVSSPGELGIIEKGCAHLQRQYLNTVRDLNHHIEIATADLQQSLEL
                     LEEKNIELSLEKKKTEEKSRQKSEFIANMSHEIRTPMNGVIGFTNVLLESKLDPLQLD
                     YVKTIKSSAQDLLSIINDILDFSKIDAGKLNLDCIPLDIRGCIDEVLSLTSPNAHKKG
                     IDLIPITDINVPKMVLGDPLRIKQIISNLVTNAVKFTDHGYVLIRTKIEQETDKDYTL
                     LFAITDTGIGISPEDQTKLFTAFNQADTSITRRYGGSGLGLVICKKLCEEMHGRISLT
                     SEINKGSTFSARIKVEKLVAYEIEKNQTHRFAHLKIICFDDNPLHLEAIGNGLGFWGI
                     EAIRVDSFNKLSRTLTKHKDCKIAFINVNQGCERQAAELIAKHKQIPFVLISKWPIND
                     FAALGARGFLYKPISIQKLQDLIESIANENQTEKNTNQELDTLREQLRFLHPEILIAE
                     DNPVNKMLLTSLLNNNANITTVDDGEMAVTACEDKKFDMILLDLHMPKLNGLEAAKMI
                     RQKSLMNKHSPIVLITASSSDLSSIDMKKYGVDFCFQKPIDEKQLLIQILRIVDKTKH
                     AAIDWQLCVQKVSGNQALAEEFLAKFIEELYKNREEFIGLMHQKNVKGLADLAHKLHG
                     ACCFCGVPILQKRVAQLERLARRTANADNLTEAFTDLIQSIDAVISEYENQYSQ"
     gene            21507..22751
                     /locus_tag="I9760_000386"
     CDS             21507..22751
                     /locus_tag="I9760_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAT5042181.1"
                     /translation="MSVKNAIYAQSGGVTAVINASACGVIQTAKLFPEQIGKVYAAKN
                     GIIGALNEELIDTSLESEEDIAKLMQTPSGAFGSCRYKLKDNGEEYQRLIAVFKAHNI
                     GYFFYNGGGDSQDTAHKVSQLGKTMGYPITCIGIPKTVDNDLPFTDNCPGFGSVAKYI
                     AISTKEAGFDVASMAASSTKVFILEVMGRHAGWIAAASGLAEQNPGEPPHIILLPEVP
                     FEPTRFLKKVKDCVNSYGYCVIVVSEGIRNKEGKFLSEAGLRDAFGHAQLGGVAPVIA
                     QLIKSELGFKYHWAVADYLQRAARHIASQTDLDQAYALGKAAVEYAVKGYNAIMPVVI
                     REQDEPYQWSIGQVPLAEVANQEKAMPPEYIAEDGMGITDACKRYLSPLIQGEAYPNY
                     KNGLPDYVRLKNQLVAKKINQD"
     gene            complement(22748..23161)
                     /locus_tag="I9760_000387"
     CDS             complement(22748..23161)
                     /locus_tag="I9760_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAT5042182.1"
                     /translation="MRMKGFTLIELMIVAAILGILVAIAIPAYQDYTIRARVVEGLEM
                     ASAAKLAVVETSLIDNTLPANQADTGYTSPAPTINVASIAIGNQGIITITYTANAGNG
                     TITMVPTIDATNRILTWDCTGGTLISKYRPAYCRP"
     gene            complement(23358..23768)
                     /locus_tag="I9760_000388"
     CDS             complement(23358..23768)
                     /locus_tag="I9760_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAT5042183.1"
                     /translation="MRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLTL
                     ADSAKLAVSETAITNNALPPTQAATGYVSPAATPNVQSIAIGANGVITITYTASAGGG
                     TIVMTPTLQANGDVTWTCTGGTLLAKYRPASCRP"
     gene            24071..24388
                     /locus_tag="I9760_000389"
     CDS             24071..24388
                     /locus_tag="I9760_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HAT5042184.1"
                     /translation="MTRKLRIEEKLISALSPLFLSVEDESMNHHVPQGAETHFKIIAV
                     SNHFNDLTRINRHKFVNQLLKEEFTLGLHALSMHLYTSDEWMTQQQSVLKSPACRDGY
                     KNK"
     gene            24463..25884
                     /locus_tag="I9760_000390"
     CDS             24463..25884
                     /locus_tag="I9760_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT5042185.1"
                     /translation="MKSWSSEKISVLALVLLITGAIDSIRNLPGTALFGSTLIFFFIF
                     SAIIFLIPVALVAAELSSTWSDEEGGIYSWVKHAFGENIAFFAIWLQWINTLVWYPTI
                     LSFIAGTISYLINPELAQNKYYLISVILIIFWSLTLIGLSGLRASATFAGFCAIVGMI
                     VPMGFIILLAFIWLIHGHPIAIDLSLSNLLPQWKNTQSWVSLTAIMTSFLGMELAAVH
                     VRNVNNPQKNFPKAMFFSVILILFTMILGSLAIAFILPKDKISLVDGVMRAFSNFLQV
                     YDLNWLMPVLILLLLLGTLGSMINWIISPAKGLLMAAKHDFLPASLCHVNKHGMPAKI
                     LILQAILVTVLCSGFLLFPTVNAIYWLFTALSTELYMMMYVMMFIAAWHLKSKFSDME
                     RPFAIPGGKPGYYLTCLLGLIGCTVTLWVGFIPPTESINIGGTAHYRMVFSLGIVLML
                     LPAALIYLRKRHITKSKALNAPV"
     gene            complement(26015..27445)
                     /locus_tag="I9760_000391"
     CDS             complement(26015..27445)
                     /locus_tag="I9760_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HAT5042186.1"
                     /translation="MKIFKFSSWLLLFVFTLDANAYYTSQGSIYDKSGNRVTINGVSW
                     SGFQDTNVFQGLQSNPFYNITMPQSKPRNGLMDLLVHPWSMADSGVNSSTAVEFKTVR
                     LPMQPGVLYDDSGEVDLNKWLSDKTQPEAGNGLFCKTWQSNGQSCEKAVSPKQAFWTV
                     LAEMKKHNINVMIDFHHRYGYGDGMRDGTVYSMVQYEKDLVLLAQEIKQRGLDNVIGI
                     DVFNEPYQLSWFKARNGQVSWITVIATAAKAVHQVNPDLLLFVEGPGGGNDDMDNPTI
                     CVPKANIKEDSGYAHWRDPGQCGNEQEVVAFKGNWGEDFKPLLDKNLAKQNIAQFDVQ
                     KFSNELQQAVPGIDTETLGWLLGDDNMGNNGHLVFSPHVYPKEVAGWETAPGDASNLR
                     FDWSWGFLYKAGYPIVLGEASWKSFEGKNFFTQALMPYLTQSGIGSNNLYFWAIGYLG
                     DTVSAINPNTGELNLDVQQTLKPYFN"
     gene            complement(27691..28443)
                     /gene="kdsB"
                     /locus_tag="I9760_000392"
     CDS             complement(27691..28443)
                     /gene="kdsB"
                     /locus_tag="I9760_000392"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAT5042187.1"
                     /translation="MSHNFHVIIPARYHSSRFPGKLLQEINGITVIERVYRQALLAEP
                     KSVIIATDHDEIADRAIQFGAEVVITSHTHQTGTDRIAEVIAKGSFAPDDVIVNVQGD
                     EPFIRPKLIQQVACSLTKTKAPVSTLCWPISSLEILNNPNVVKVVCTRDNHALYFSRS
                     AIPFHRDDKNAYSNTFRHIGLYAYRAAFLLEFVSWPPCTLEQIECLEQLRILWSGFSI
                     RVDEACEEPLQDINTKEDLILAQQYFLDTFNV"
     gene            complement(28440..28619)
                     /locus_tag="I9760_000393"
     CDS             complement(28440..28619)
                     /locus_tag="I9760_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019231979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HAT5042188.1"
                     /translation="MDKKLLEILVCPLCKGKLLFKKQELICKFDRLAFPVRDDIPVML
                     EQEARLIPLEEKDKL"
     gene            complement(28612..29283)
                     /gene="tsaB"
                     /locus_tag="I9760_000394"
     CDS             complement(28612..29283)
                     /gene="tsaB"
                     /locus_tag="I9760_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAT5042189.1"
                     /translation="MMKLLAIDTSTELASVAILIGDEIISREQDSQRIHAQLILPMID
                     ELIAQTGLGLNQLDGIIFGCGPGSFTGLRIACSIAKGLAYANDLPLVPVSSLAAIAWT
                     AREIKEDFNQPVLSVLDARMHEMYWSCFLEHQFLAQDRINAVKDIQLPANQSFILAGV
                     GIDLYWKDFPEQIKSQISEVLAVFPTASAMIRLAQKANIKAVSVAQAQPVYVRNQVTQ
                     GDSRG"
     gene            complement(29261..31219)
                     /locus_tag="I9760_000395"
     CDS             complement(29261..31219)
                     /locus_tag="I9760_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAT5042190.1"
                     /translation="MEEITTFAETCRRTLSEKGRLSTAIPGFVARAPQADLAVAIANA
                     IVQRSILVAEAGTGTGKTFAYLLPCLLSGKKALISTATKTLQDQLFQKDLPTLIRALG
                     LSVRIQNLKGRSNYICQYRVEKHAEEGQFQSPQCAHEILHVRDKLSQMQEGVRSELPE
                     INEDSSVWHYVTSTTDNCLGAECPNHETCFLVKARKRAMEADIVVINHHLFFADSQLK
                     KDGFSELLPGVDVVVFDEAHQLADIASNFNGERLGTRQFKDLVDDMLNEWPILDLANQ
                     PLKSISHKADKLVDQLLNALPTREERISWDLVKRNKEFMEAWNTWLSLKDELIQCLES
                     KDTADIPGLKRCKERLLDLETVLLSFTEENNHKIRWIERFKHTLVFHATPYDVAESFS
                     QLLKDQSCAYIFTSATLTMASVFDSFCKPLGLDKVQTLLLSSPFDYQQQALLYLPRGI
                     PDPKDAHYYEALLEKALPVIEACGGRCFFLFTSHKAVKLVAQMMSNTLKYPLLIQGDE
                     AKPILLSRFRQLGNAVLLGTSTFWEGVDVKGEALSCVIIDKLPFANPVDPVIRGRMSY
                     LKKKGLSGFDELSLPNAVIALKQGVGRLIRDITDKGILMIADPRLTGREYGRTILASL
                     PGLPKTRDEEKVLHFIKELALNDETVSD"
     gene            complement(31207..31542)
                     /locus_tag="I9760_000396"
     CDS             complement(31207..31542)
                     /locus_tag="I9760_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAT5042191.1"
                     /translation="MTFVVTESCIRCKYTDCVEVCPVDCFYEGPNFLVIHPDECIDCA
                     LCEPECPVNAIVSEDDLTEEQQQFKELNAELSKTWPNITAKKDAPSDAKDWEEVKDKL
                     QYLQKEWKK"
     gene            31825..34617
                     /locus_tag="I9760_000397"
     CDS             31825..34617
                     /locus_tag="I9760_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1924 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042192.1"
                     /translation="MPLNLPPKSSKNTMPLVIAYNNAPEDDKIQKLFYLQKINYLLNK
                     TQLNDDLFDWINDAEEGGWLNELAKFSINPNASFFLKGMQFAKAITEEIKNKPEINSS
                     EVNIYHLMQERDQLLKEVEFEKCATRYAEINFLLNELALNDKKTKEIVERQTEILRLV
                     APKIKAIKGESIDNLPVIPSYKTKELGNHVNNFNFKFTMSGWEAPFVFRVEDRHELGK
                     EQELHSYGVSKYFIEDYSVFMMRFKAEDGSTVYKPVILSQFANQNNLEEIAKQLKDGS
                     PKNIAPRIGYYFVQLTDFCLKLIETHNYHPDIKLNNFLVHNNRVLVSDRKTFTTNDNP
                     LASEILTSPLFAPDEFLKCLLFNKEGDPVGYNRNALWKRMNMPQFMAYQLGMALKQFL
                     ILTQLDELPDDFRNPDHSAVSHFKTPSRQIINLSLLVQELTRLDPDKRMTIKQFQTLL
                     NFKNLPPDAFYQKVEEVFPSSQLGIAEDIEALNKVLNSDLKGEALLKQANPVFTKLSK
                     YDPKETRLTRLAEKLAIRCFNNVSKPYFQKLSGLIESALEKDVNEIRQLLNPQSNSER
                     LLQKASPIFMKLFERQPEVPQLTDLAKQLAAQCFDESSKNYFTRQLPLLIEEELLNQD
                     WEQAPWYRKALHWLTFGYFRVDNVTEISSLKIPEHTKGKEFQIYFWQLLFLPSEEIKN
                     MFTSKDSQISEFLLGYIEKEREKRETETPSVQIPVDLKGEEFQIHFPQLEFLPSKDFE
                     SIGEKEGEHLECFIFANLQEILSHNNSDSSEKNSTSEPSDSESEIHLNAGNVDTIIIA
                     NEPNPTDAKPQKKDNDGAKQNNPAKASSPEGIVSTPEVEEHPKKKITDDAPKPKTKRS
                     VHFFDEAKKSKPKEKENASPAVDETPPAKRKNVCRIDSVRSTLFRGDGSHRQKIKEQR
                     PRLSEIAWEPPKPNLQ"
     gene            34934..37579
                     /locus_tag="I9760_000398"
     CDS             34934..37579
                     /locus_tag="I9760_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042193.1"
                     /translation="MDVGQKIRILKRYLKEKSITPEDVNLNDQDLTAYAGLDMLLDYI
                     SIHHNGLFDFATLESDEKQWKQYVDILNYSATYSPGSPPALIDTSERKAQFGLQYWLN
                     TRTIEALPRDNYYTRTVTLSDEADEEFDQLITESRAKLYEIAQTFPEISSFNPANIIQ
                     VTNQISSIKEKLDTLTGNNSQITLLQKKLNVLTDLNKINQLERLNIPQDNGSNLSYCT
                     LAETIEGVTEKIPLITLAKVKEQLRHLCAQNELDLDSYSESREQYYKLGIEGILKLPK
                     HGDIKEVQREAIALNISRLMGLDTTSSTTVSYNGHPALFIPFDNINLLNEFSSGKIFK
                     VGMGLSGQSYTHYSTIKPLGEGIQADLYIKDFGHALALLYLCCDTDAIGGYCKNKALR
                     NYSNLYIFDQVITDTDKFILDSRISLQPDQFIMKHTRHGQGRNRTLIEDSSLVTKYAS
                     LMRLKELKRIITQYCDHISWLHHNRTQAIKKQLKEELAKDERVRLTEELNDVEILEKD
                     AEQIKNQILIRINKIAEVLPQTTGPVGSDVIRQCLILEKLLHNPVLYSDDGRPYKHPW
                     TTRQANTVQTINDLQNGKIQITFKFNIPEEMVDFIKRHGGGDSLTISSPKVITISKAH
                     LDSLRENMLHPEHSPELSLNTDYLDDKDLCIIKMAYRIGNRTKILNAIAEYRVLMNNE
                     QVSFHNKIECLAQTEAKIKALIELANDKGLGMHILKKFYFDAQQQLQKLINPEVLPHD
                     IGQAFAAALRLDRVSEFNKVVREAIVQNKVTDPQFTIFLTSCIKNESLAKNYKDAQKT
                     SRALLIESQRIINHLKSPIIPLIVQLGGQLHSQAELDTDGLANLENDLQLDFEKPDTE
                     SMTTMDLHQAVTKEEMIENGMTVKV"
     gene            37655..38212
                     /locus_tag="I9760_000399"
     CDS             37655..38212
                     /locus_tag="I9760_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HAT5042194.1"
                     /translation="MPVHERRQHFRIDDHIYFDYRILAPGEFCSDIEITNQLLGENGQ
                     KYMEAAQYFQGIDYELAELTQVMAVKEPALAHYLNLLNAKIDYLSRQMLMSSKVQLRK
                     VNISLGGMAFKTMEQIREKTNLKIILYTKPKMIPIIVDAVVVYSQYQSETHYRTAVAF
                     CNLTSEQEQLLSQHILQAQIKSRAD"
     gene            38288..>38459
                     /locus_tag="I9760_000400"
     CDS             38288..>38459
                     /locus_tag="I9760_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAT5042195.1"
                     /translation="MSRTVFCCKLKQEAEGLEKQPFPGELGKKVFNEVSKQAWNMWLS
                     HQTMLINEYRLNL"
BASE COUNT        12364 a         7688 c         6954 g        11453 t
ORIGIN      
        1 gcccatgtcc ctgtggaagc ggtaaaaaat ttaaaaactg tcatgataaa tgaattaaac
       61 tcaaggtagc agatgaacgg tcacttactc ctcaatgttt tcattttcct tgctgctgca
      121 agcatcatgg ttccactggc aagccgattt agacttggtt ctgtgattgg ttatcttacc
      181 gttgggatgc tcattggacc ttttggactt aagctaatag gcaatgccga acaaattatg
      241 cattttgcag aatttggagt agtcatgatg ttattcttga ttggtttgga attagaacca
      301 atcatgctct ggaagctgag aaaattaata gttggcttag ggggcttgca agtgttgtta
      361 acaacaatcg ctctgtcttc ggcagctgtt atgatgggat acaatttgcg agccagtgtc
      421 gcggtcagta tggcacttgc cctatcttca actgctttag tcttgcaaat gctccaggag
      481 aaaaatcttt tgaaaaccgc ggagggtgaa acttcttttg ctgttctctt attccaggat
      541 attgcggtca ttccaatctt ggtgattttg cctttattag gcaatcatca tggaattaat
      601 gtggctatta accctcatac ggctgcctct attgctcaat tacccaagtg gcttcacgct
      661 attgtcgttg ccggcattat cggtagcatt gttttggcgg gacactactt atccagatac
      721 ttgttcctaa tcattgcaaa aaccaatatc cgtgaagtat ttacagcatt ttccttatcc
      781 ctggtcgttg gaattacctt gttaatggaa acaattgggg tgtctccggc tttgggagct
      841 tttatcgcag gtatggtttt agctaactct caatataaac ggactgtaga agctgatatc
      901 caacctttca aaggactgct tcttggatta tttttcatct cagtcggaat gggtatcaac
      961 ttcaatctac tcttcagtca aatggctgta atcatcccta ttgtactcgg tttgattacc
     1021 attaaggcat tgattctctt tacgctcggc aagatatttc aactcactaa aatacaggcg
     1081 ctcggattcg cccttggttt atcacagggt ggagaatttg cctttgttct ttttcaatac
     1141 tccagcacat taaaagtaat cagcccggaa ataaacagtt tttttactct ggtagtcgcc
     1201 ctgtccatgt tggccacacc ctttttaatg cttttgtacc atcgttttat gataccgaaa
     1261 ttaataagca aattaccaga acgtgaatac gactctattc atgaaaaaaa taacattatt
     1321 atagctggtt acggtcgatt tggtcagata attggtcgat ttcttagtgg tgaaaacatc
     1381 aaggtaaccg ttttggaaaa aaatccggag caagtcgaat tattaagaaa atttggctac
     1441 accggatatt ttggtgatgc gaatcgtctg gacctgctaa aaagcgcagg agcagcgcat
     1501 gctcaattgc tcatagtcac agtcgggaat cccgacacca atttggaaat tgtgaaatta
     1561 gctaaagaag agtttcctca attgaaaatt tttgcccgag caagaaatag acgacacgcc
     1621 tatgagttac ataaagcagg agttcattat ttcaaaagag agttatttga ctcttcgtta
     1681 accatgacca aggaaatttt aaaatttctt ggttataaac ctgaagaaat tgagaaaaaa
     1741 gccaatgctt tccaaaaaca cgatgaagaa acattaatta aatcttttga tttttttgag
     1801 gaagaagcca atctcatcaa tttctcacgt caggcaaaag gagaattaga gcgtattttg
     1861 caaaataata atccataatc aaatcataaa ggtctatact tccatataat catcttgaaa
     1921 tggctttcat tatgataaaa caattacaac aggcattaag tgatattctt ttaggaaaag
     1981 aagaagtcat cagattgggt attacctgtt tattagccaa tgggcacctg cttcttgaag
     2041 acaacccagg tatggggaaa acaacattta gtcatgcgct ggctcaactg actggtatgc
     2101 aatacaggcg aatacaattt accagcgacc ttttgccaag tgatcttttg ggagtatcta
     2161 tttatgatca tcaacgcaat gtctttcaat ttcataaggg tcctatattc tctcaattaa
     2221 ttctcgctga cgaaataaac agggccacac ctaagactca aagcgcctta ttagaggcaa
     2281 tggaagagag gcaagttact actgatatag gaacagaagc tctccctcaa ccattttttg
     2341 ttattgctac tcaaaatccg tcttgccata cgggaactta tcctttgcct gaatcgcaat
     2401 tggatcgatt tctgatgcgc ctggtcattg gctacccacc gccagatgcg gaacgtatgc
     2461 ttttaaaatc atcaaacttg aatgctcaat taaaggaact aatccctatc attaatcctc
     2521 agcaattaca aattttacaa agagagagtc aaaaagttct agtctctgaa ttggttcttg
     2581 attatgttca atctctactg gacgccagta gaaaccaggg atggtttaat catggattaa
     2641 gcccaagagc aggaatggga ttagtgcacg ccgctcaagc ctttgccaga actgatgata
     2701 gagattttgt tcgacctgat gatattcagg cagtcatgcc agctgttgtg aatcaccgct
     2761 taatcataaa aaaatcacaa actcaactct ctgctgccga attattaatg agtgaaattg
     2821 aggttcccgt gtgataaggc aaatcaaaac aaaagttatg gcttattggg aagaatggat
     2881 tgccaaacga atctctaaaa ccaatcccca aattcttggt tcacaaaata tttatattat
     2941 accctctgga tttggatggg cctatggtgt ggttgtactg tccctgtttt ctggcgcaat
     3001 caactaccaa gttagcactg tttttttaat gagtttcatt ttagcaattg taggttttat
     3061 aagtgcttgg gaagctcatg ccaatcttaa aggattatct attaaattgg tatcggttga
     3121 ggatacctat gaaggaacgc cagcccaggt tagcttattg attcaatcaa ataatagaat
     3181 gcattttgga ctagattttc gccttaataa tcaatcagtc actcgattag aaaaaattcc
     3241 tccgcaaggt ttgcgattta tttttcctct ggcaacaact cgaagaggat gttataccct
     3301 acccaaaatc accatttcca gtttttttcc ttttggcttg tttcaggtat ggggatacgc
     3361 caattttgat agcaattatt atgtctatcc tcaacctgtc aatcctggct tttggcccat
     3421 acccctttat catcagaata aaaggaaaac agactctcat ggagataatg aatactatga
     3481 cttaaagcag gtagacaatc cctggattca acctaatctt attgcatgga aaattgcagc
     3541 aaaaggacaa ggttggtatt tgaaaacgat ggaccgcgct gaagaaatgt attggctttt
     3601 tagattaaat gatttaccta ttgaaacact cgaagaaaaa ttaaaacact tgagctattg
     3661 gttggttact gccgaatccc aaggccagtt ttacaagctc gagttgggaa acgatccgga
     3721 tcaattttcc catggggaag aacatcttcg atattgctta cgtcaattgg cggtgtattg
     3781 atggactttt tttccaaaca acatactttt ctgataacaa cccgatacgc tcttttggtc
     3841 atgttgatat gctactttcc ccatttttta acatgccctt tgtggcttac tgccctcatc
     3901 ttgacaacgc tatgttacaa gttaatatcc gattactttg cctatccact tcttgacggc
     3961 aagatacgct ttattgtggt tatcatctgt ctttttctat taaaatttca atacggcagc
     4021 atcatctcca gtggtttttt tatcggtttt ttactggcat ttattggact aaaaactatt
     4081 gaaattcata atgacagaga tataagaata ctaatactgt gcaattttta tcttatcttt
     4141 gccgcattaa ttgtggtgca ggaactatgg atcatacctt atttattaat agcaattctg
     4201 gctaatttca ctctgatgtt aaaattaact gctcctcaag cctcactaag acaaattaac
     4261 ggccatagta tgaaactatt acttattgca acaccattaa ctgtctttct tttctacttt
     4321 ttcccccgca tagccaatcc cctttggcaa gtaccttccc ttgagaaaaa tcacactgga
     4381 tttagcgaat ggatggaacc agggtcggtt gcaaatcttc taaatgatga caggactgct
     4441 ttacgaatta tatttaaaag aaaagccatt ctaaatggct attggcgagg attaacttta
     4501 agtagataca atgggataag ctggaatcct gcctggcatg actcatcaac cttctcttca
     4561 ttaaaggaat taactacttc tgatgacggg gattatgagg tcattatgga gccccaccaa
     4621 aaaaagtggt tgttttatct tggttacccc aatgcggctt accctcaact cttattttct
     4681 ccaaattttg gtttggttag tcataataaa gacatgattg cccaacgttt tgcctatgct
     4741 ttgcgaatta gctcttcccc tcactcctca ttgacgcaga aagattggaa actcaatact
     4801 caattaccta aatattcaaa tcccagaatt acgttctggg caaaaaaaca atttgccgaa
     4861 gtaaattatg acccccaagc ctttattcaa ttcatccaga attacataaa aaaggaatcc
     4921 ttctggtata ccctaactcc tccaaaactg gataatgaaa atgaccaaat ggattatttc
     4981 tggtttgatt ccagaaaagg attttgtgaa cattatgcaa gcgcagttac tgttgtatta
     5041 cgcgctgttg gtatccctgc ccgggttatt gttggctatc aaggaggtga atggaatcca
     5101 ctggcaaaat atttaactat aaaacaatat gatgcacatg cctgggtaga gtattggcaa
     5161 aaagatttcg gctggaagca actggatccc actgcattta tttccacctc gcgtatcgat
     5221 ccttctgttc ttgccatgcg agcggcacgc accgatcaac agttaataat tgatccctta
     5281 aacttaaact ggattcagaa aatcaaatta tatatggagt caacccgttt ttttatcgag
     5341 cgttggctgt tattttacaa tcaggatgct cagtataaat tattagaaca aatcggatta
     5401 aaacattgga atgccgccat gctattgcaa attgccatat tgtctctggt tttatttatc
     5461 attctcttgg gcgcctgtta tcaatggtgg caaaaaagga cattagatcc cttattgcgt
     5521 gaataccatc ttttacaaaa agaattttgc cgcttcaata ttgcaactca accacccgcc
     5581 acactgaata aacagtgtaa aattttaatc aataaaattc ccaatctgga agaaaccatc
     5641 actacctttc tttctcacta tgaaaaactg cgtttacgac accgcattac caaaactaaa
     5701 gaaaataaaa aacaaacgct tttgttgttt aaaaacctga gacgaatatt aaaacaagta
     5761 aaaatctaaa tttgacggga tttatcggtt gcattcttga gtctcaaagt gcttaaatag
     5821 ataacattta tcaaactatg atttttaata atggttcact ttcaaaaaat gactgtccaa
     5881 acctttttaa aagactattg gcaaaaaaaa ccattgatca ttcgtcaggc tttacctgat
     5941 tttactaatc cactcacacc ggatgaattg gcaggacttg ctcttgagga agaaatagaa
     6001 agccgcctgg tctatgaaac gcccgatcag tccccgcagt ggaatttaaa aagaggcccg
     6061 ttcaaggaat ctgatttgat aggcttgcct aaaactcact ggactctatt agtacaagga
     6121 gtagaccgta ttgttccaga tgtctatgag ttacttgagc atttcaattt tatccctcaa
     6181 tggcgagttg atgacgtgat gattagctat gccactctac atggaagtgt gggcccacat
     6241 tacgataatt atgatgtttt cttgtatcag gctaaaggtc ggcgactatg gtcattaacc
     6301 tcaaaaaaat gccacaccaa caattttata aaaggtcttg aactacgaat catgaaagaa
     6361 tttgaggtag aagagcaatt tatcctggaa gaaggcgaca tgctctattt acccccccat
     6421 ataggacatt atggcatcgc tcaatctgaa gaatgtatga cttactcttt tggttataga
     6481 agttaccagg gacaagagtt atgggatagt ctgggtgatt atttgtctga acatggctcg
     6541 ttcaaatccc tgtatcagga tccggattgg tccactctta aaaacacatc ggaaataacg
     6601 cccaaagcat gggacaatgc aagacaattg ctaagacaag tcctggaaaa tgatcagctc
     6661 atgcaatcct ggttcggttg ttttgctaca agccttgatc aatcagcaga acaatattta
     6721 ccccctcctt tagaagaaga tgaattactc ggtttggacg aatttattaa agaattggct
     6781 aattatcagg aaatagttag agatgcctca tgtcgttttg cttatatcat gtctgaccaa
     6841 gaatcccagt gccattttta tgtcaatggg aaagaatggg acagccgtgg ggtttcaaca
     6901 aacttactca gttttgttgc caataatcga ttcctgtctc taaaagagtt aaaaccttat
     6961 ttaaaccata aggctaatca actcttcctc tatgaattat ggaaattaca atggttacaa
     7021 atttctcatg atcaatgatt tggtttataa atatcttgaa taaaattcac tctcaatagg
     7081 tggacaaaat aaaattttct aattagttta ttagatcatt attatatgga tatgtaggat
     7141 aaaaaatggc gcgcttttcc agaataagtt taagtactgt attgttatct ctaatttgtt
     7201 tcaatatttt tgcctctgac actattatga ttacagttaa taccaaagaa aaaaatgcca
     7261 ttgctatagg ttactcagtc aatggaaacc aatatggagg tttgggcagt tcctattctg
     7321 gacatggacc taaaaataag gagtatcaat ttggatataa gaaagattcc atttttggaa
     7381 aagatatctc ttgtggttct atgaccttaa atcaagacag taaggtgact ttggtaaata
     7441 ataataacca atgcatcagc gttttaggtt aattggctat tgcatggaat gtagatatta
     7501 attttgaaaa gacggtagtg cctatccaac aatattccac tcaaaataat tgaattcaac
     7561 tcgtagaaac tcaaatctgt aattaacagt ttatcccggt taaagcagta caaacctctc
     7621 tcctaaacca gacatttagc aaccaaaaat accaaaaaac acaatcaata caatcaattg
     7681 gataatttta aattcatata gtatatttaa tatacatgga ccaaacaagg ataggcaatg
     7741 aaaaaaataa tattggccgt agttgttatt tctgctgctg tattattgag tggctgtgga
     7801 agtggatgct gcggtgccag caattcatgt tgcagctata atactggatg ttgtggtgta
     7861 caaaattatt ccggtatttg gtattgattt acaattataa aagccaatag tctgaaatat
     7921 gaaaaaaaac agcttgcttg ccatctcgga agaagggttt caccatattg cttatactga
     7981 atggggtatc tttgaaccag agttacctac agttatttgt gtgcatgggt atactcgcaa
     8041 ttccagggat ttcgatgaat tagccaatta tcttagctcc aagggacgtc atgtattctg
     8101 cccggatatt gcgggtcgag gtgatagttc ctggtttaaa aatccccgct attataattt
     8161 tacccagtat gtaaaagata tgacagtcct tatggcaaga acaaatgctc atcaaatcga
     8221 ttggattggg acatcaatgg gcggcatcat tggcatgatt ttagccgcaa tgcccaattc
     8281 cccaattaat aaacttgttc tcaatgatat agggcctcaa atacctattc atgggttgag
     8341 aagaattgct aaatatgcag gtaaagcccc tgattttaaa agcctggagg aagccaagca
     8401 acatttcaaa gccagctatg ctgattttgg aattacaaat gagaagcagt gggacatttt
     8461 cactaaaaac agtgtagaac aacgaggccc taatctttat gtcaccaaaa tggatccggc
     8521 aataaaaaaa tcaaaatcaa ttttacaaat catttcagaa ttttttcgtc atccccataa
     8581 agcactggaa ggaatttttt atgacattaa tctgtggtca atttggaagc agattaagtg
     8641 tccagttata gttattcatg gtgtgaattc tgatatcttg acttcggaga taatcacaca
     8701 aatgaaaaaa actcatgctt taactgaagt ttatgaagta gaaaatgcag gacacgcacc
     8761 agctctattg gatctgactg ttcaccaaag aattgaagat tggcttgcca ctcatcaata
     8821 aatcacaaaa gaaatgccgc atctttatta tcaggacttg tatattgcgc ctagccattg
     8881 atttaaatta attctatgac taaacaaggt aatcaaagcg cctgccaaaa ccaagacgat
     8941 acccaacgca tgccaaggct tgagttcctc accgagcagc actacgccaa agatcaccac
     9001 aaaaacaggt tctaaaacgg ataagataga tgccttttcc gaactgatgt acttcaagct
     9061 gtgcagcatt aacaaaatcg gtataaccgt tgcaacaagg cctatgccaa ataaattcag
     9121 ccaaacaggg aatgatgtag ggactttaaa ggtatggtcg aggcaagcta caatcaaact
     9181 ggttgtcata cagcctaaac ataccatcag ggttgagaca tttggagaaa tttcattgtt
     9241 tttacttgaa actatataac acgcataaaa aaaagctgaa gctaagccca gaaatatacc
     9301 ccataaatca aatgcgattt catttacatc aattaacaac gccattccta tcaggataac
     9361 agatatggcc aaatagtata tctttggtat tgtttggcta tagaaaaaat aattgaacac
     9421 cataataatc actggataag taaaaaatat caccatcgat aatccagagc ctatatagag
     9481 ggaagcataa aagtacagta atgtcgataa accataatag aggacaccac ttaaaaaagc
     9541 ggtaaacatg gatttatagg tatcattgat attttttaat tgaggaatca aaacaatcaa
     9601 aataaaaata ctggaaataa gaaatcgcca aaacaacata ttgcttgctg ataaatcacc
     9661 atggatagca ctaatcccaa aataaccaat aaatccgtaa agaaatccgg ataaaatagc
     9721 ataaaaagat ccacgttgtt cttgggagtt catgattgag ttactcgaaa taagaggaat
     9781 gatctagcaa ataaaaagta ttattttact acttttattc tattttgaaa attaataatt
     9841 caaatttaaa tcaaattgtt aattgttaac ccgttaatca aaggctattc atcactcgac
     9901 aaaccgatat ctcacaatgt tttgtccagt ttatttaaac acactctatt gacaacaaaa
     9961 tatacacaat gtaccattcg aaaacttcaa ttttggctct atatcaacta atgtttcgag
    10021 ttttaatttt tctttcttgc ctagtaattt tgacgccaaa cactattgct gacactaacc
    10081 cctgtgaaaa acactcttgt atagctgtca ttgatgccgg aagtactggt tccaggctac
    10141 atatctactc ctatgatacg gatgacacca atactccaat tcatattgag gaaatttgga
    10201 ataaaaaaat caaacctgga ttcgcaagca tacagcctaa ttccgtaaca attgatgcct
    10261 acctgacaat gctattggct gatgcaccaa ttcataatat acctgtttac ttttacgcaa
    10321 cagcaggtat gcgactacta ccccaatcac aacaaaaaaa atactatgat gaattggagt
    10381 actggtttag acaacaatcg caatggcagt tggccgaggc aaaaactatc actgggaacg
    10441 acgaagcatt gtttgactgg cttgctgtaa attataaact tgatacatta aaatcagtcc
    10501 aaaataaatc agttggcgtc atggatatgg gcggcgcttc cgttcaaatt gtctttccaa
    10561 tgcctaaaaa tgcggagatt agcaaacata accaggtaga gctcaatatc tatggacaga
    10621 atatcaatct gtatgttcac agtttcctgg ggttaggcca aacagaaatg tcacatcaat
    10681 tccttaactc cccctcttgc tttgcaaacg actatccttt gccggacggg gagtcaggac
    10741 agggcaatgc tccatcgtgc aaggaagaag taacctcatt aatgaatagt gtccataagg
    10801 taaatcaaca aatacaacct ttgcttgctt taaatcccgt gaatgaatgg tacagtattg
    10861 gtggtatttc caatcttgcc agcagccaat tatttcattt tgaaaacagc gaactcacta
    10921 atcaaagttt gctgcaacaa ggggacaacc aaatttgcca tcagcaatgg gatatactta
    10981 atggccagta tcctgacgat gagtacctct accaatattg tcttttatcc tcttattact
    11041 acgcattaat ggtggatggt tatggcatta accccaatca aaccatccat tatattccgc
    11101 cagaacaaaa tctcgattgg acaattggag tcgtgcttca ccgcgcttga agcattacaa
    11161 ttcatcaatt gaaaaatcat caattctcag gggagaaggt tgacggtaaa aagcacataa
    11221 agcattacga aaatgcagtg cacgctgtgg taacccctgg gtacaataat gaaacacctg
    11281 attatagatt gactgtttaa aggcctcagt gtctacttca gtcccattta aattatcaat
    11341 acttacccta aatttatgtc ccgcggccat agaaagaatc cactctaaag cctgtggctt
    11401 cacttccacc ctctgaaaca gagtttgttg ttcctcattg cgtccatcag gagcatacca
    11461 atagccaaaa tcgaccagct ttcttctttc ttctccagca ataagccaat gagcgcattc
    11521 atgcaaagca ctgctaaaaa atccatgtgc gaaaaatagc gcatgataag gcctcttatc
    11581 atctgcagga agataaatag gctcatctct ccctctaacc agacgggtat tgtagtcctt
    11641 ctcaaagcaa gaattaaaaa tactaattag atcttctacg tgatgcacga atcgttacct
    11701 agcaaagcct ttgatgctta cctatctgat actgattata ctcttttttc aaagcagatt
    11761 ttaattctat ttcattttcc atacctattt tggctgccaa agttaaagaa tgcttgatat
    11821 gcacagcctt tgccttgggt atctgaagag aaatgacttc gtcatgcgag gcagaattca
    11881 tgcttgctaa taaggcttct tttttagata accctccaaa acgtttctcg ggtttatctt
    11941 taagaggaag aaactctttt tgtttcagaa gacaacgagt tacctgataa taggtattaa
    12001 tttgcttata agttaaatcg atcgcagttt cgttcttata gatgtccatt tcaggatttc
    12061 tgggtgtaga tggatgatga agtttggcca gttttagctc attctcatcc ggtacctttt
    12121 ctttaccttt taaaatgaaa tcaaacatcc ctgcccaagt gtttttgtct tcttcattat
    12181 tacttaagcc aaaagacaaa taaaaaattc cactggatat tgcaaaaaca gcaagagtac
    12241 ctattgaaac aatagcggga ataattcctg cggtacttaa ccctataaga gtaccaatag
    12301 tcagtaaagg aataataccg aatacgatta acggccattt ctctgcaata tagtgtatta
    12361 ctggtctgat taagtaggta taaggagcca ggccaaaata ataaagaact cctctcccta
    12421 aagagtaaag ccagaaattc cttgctttcc ttttggcatt ttgttgctca gcagaaacat
    12481 aagtaaattc tgtttcagca aatccggcat aattcagaaa atgatcacaa aaaagattgg
    12541 ggccttgttt atcttttgga ggcttgacat gtaaaatgtt ccactctttt ttccaaaccc
    12601 caaaaaaaga caccggatcg ttaccttgct gttggacaac aaccaaaggt ttctttttga
    12661 ttttatacca tttatcgtac ttcactttat tatcagtatc atgaagtcct gcggggttta
    12721 aggcatccac tctggaaagg tattttccca tatcaatagc caaaagcaga cttaaagagc
    12781 ctccaagact tataccacat acatgaattt tactgttttg ttttaaaatc cagtccttaa
    12841 ttctatccct tccattttta tataaagaat agccaaccgt ttcaaatcct ttcaaatcag
    12901 tatttaattg ggaaataaag ccttgacctg caggataagt ggttcccata aaaattaaat
    12961 gagactgggc atttggatta tctattggct caagtccata agcaaaaacc ctatcatcat
    13021 ctcgcaaaat cagatcattt tttccacgat gatttgtaag ttctatgggc ttgatgtaat
    13081 agtcaaccat aacccattga tcattgattc tctggggaac tttaatcgac tcgtaaggat
    13141 ttaaatcaaa taaaggcaaa taaccaagac agttactaag gtaaagttgt atttgttcat
    13201 tttgttgcga attaaattgc ctgccactta attgggtaaa tagtgcatca aaacctttct
    13261 gaaaggctaa tcgaaactct ttcagcaaca gaggatctct ttgaaggtaa atagccttaa
    13321 aggtttccca ggaaaataat tctgtccatg aggtattcca acccatcatt aacaatcgca
    13381 aagtattacg aataagaaca gccctttgct cttcaggagg tatgtttttt ctagattcga
    13441 actcagcatt ctcaataaaa tttaaattca aaccgccttt aaaacttgta cactccctaa
    13501 ccataaaaaa cctctgaata gcttgtttct aaccatgagt tcaagataat tacaatcact
    13561 ggctcgcaga ctgctaagcg agtcatttaa aaaccaagta actatatatt actatcaatt
    13621 aaaagttgaa attttagcac aataacaaag attattgcta ataacttaaa cctaaagggt
    13681 ttattttgcc caaaaggatg ggctatctaa caagaaagtt aaacccccat acttcaaggc
    13741 attggatata atgaatctta ctcccagtac tatatagaag aagcaaattg ggttgtaatg
    13801 aattaataac gcaactcgcg ctatagtttt tcttgctgca gtgacttggc aactgaagag
    13861 cgagccagca taagatccat aaattttgtc aaatgctcaa actgagacaa atccaatttg
    13921 aaatattttg cccagcttac cataacaaac aggtaggcat caggtaaact aaattcattt
    13981 cccatcaagt aagatccttt ttccaaacgg gtattggtat aagcaaaacg ggtcatgacc
    14041 aaaggtgtca aaattttgga ttttatttcc tctgaaacag taggattaaa aaacatacca
    14101 agcgacttat gaagctctgt agaaatataa tttaaccatt ccagagtatg atatctttta
    14161 ctctctccaa cagcagccaa caatttttgt cccggagtag tatctgctag atactgtaga
    14221 ataacctgat tttcagtcag gatatcgccg ttatcaagaa caagggttgg gaccgctcct
    14281 ttaggattaa ttttaaagaa atcatcgcca cctgctgttt ttttgacttt taaatcgacc
    14341 tcttcatcct ggaagtttaa ccctaaatca ttgagtataa tacgaactac taatgaacaa
    14401 gcacctctgc aataatataa tttcatgttc actcctttga tgatgtttaa ataataatag
    14461 ctaagttagc ataaaatgca gcaaaaaact tgaaatgtaa aaaaccatac agtgaaaagg
    14521 atgagcttgc atatggttta tttaatcaga ccacgtttaa tcaggctcta taagaagcaa
    14581 agattcgata tgctctaacc ctgatattat cataacaata tttaatatgg gcacttggcg
    14641 catttgagat ctttctcaat ttcattgatt aatttcatag tagctgattc cctgacaatg
    14701 aaagaatccc aaaaataagt tcttcttaat gtttgcaaat caactcgatt tttttccttc
    14761 cattgctgta taccaaacag caattcatca atagattggt ttttctgtga accaatgatt
    14821 tgcaaatata aattttttaa gctattaaat tttcgttttg tcattggtga attaggctcg
    14881 agcacaggca actgctctat tctatgatag agtttcttgg ctattaagcg tttttcttta
    14941 ttaaatcctg gatggataga aggtggaggt gggggtaaaa cataaaaggg aatattttga
    15001 ctcggtttgc catagtccaa ttgagtttta tacggcagat ttataaagaa acaagaggat
    15061 atcgatgagg ggacagatgt ttttgtaacc gcctcaatta attttattgc tgaatgtcga
    15121 caagtattcc ctgtattgat ttcatgaatt tcctcattaa gctccttcca atgggaacca
    15181 gttgcgaaaa cacgagaact ggtttgcgaa aaatagactg catttccatt ttgaacaagc
    15241 ggtttaaaac actcaaattg attgctctca gtttgaatag actcaagata atgaacaaat
    15301 tcacaatact gctcataact aatatcatag gcttgataag aaataggtgc agatcctttt
    15361 cgttttcttg taattccttc atcctctatc tttgctttgc tactcgcaaa aattgcccca
    15421 cttaaagcac tataaatgct aagacacctt ttaataccct tggtttgatt aggcagatca
    15481 aagattttcc ctactctgca cagtagatga gatacctgaa agtcatcata cacacctagc
    15541 ataataaatg agtgctgatt gattttacct atcgaaataa aaaagtgatc tgtttcttta
    15601 ttaatacaga ttgccatgtg attcagtcct taacaaccaa taatgataaa taataaatta
    15661 gcttgcacat tgtaggtgtg gatggttttt aacgataact ttatgtatat tcaaacaaag
    15721 ctcatggata tggttgccga tttttgattt ctcattgcct gagccacctt tcccattcaa
    15781 agccacaaca accgttacat tattacaagg attccacaaa tacatcaccc tgtaacctaa
    15841 agtactgccc tggtaagtcc aaaagcgctg cctggaattt ttatcataat aaaaaccaac
    15901 tcccaatcca aaaccgtagg gctcatcttc tgtgactgaa gggataggta accctgtttt
    15961 tattgaaacc actgactcca attcggataa agcctcgtct ctaaaagcag gttctatcag
    16021 taacccatgg tatagcagct gcacccaacg accgacgtct tcagtatttg cgataacagc
    16081 acctgctgtg ccagcccagg ataaatcatt gcttatagta tcaattagtt ttttgttgtt
    16141 ctcatcataa aagtaaccat ggactcgtct atcccggatt gatttttcga cttctgggcc
    16201 atgtggccct gcaggataaa atgaattgtt taatccattg tttgtattaa taatcctttg
    16261 ttttaactga ttttcaaaag tatcttgagt cactttttca ataaccattc cagcaagaat
    16321 atagttagaa ttggaatact catacctgtt tgctctgtcg gtttctattg gcttttctgg
    16381 atgagcataa cctaacaact cctcatcgcc ccagattgcc gcaaaattat cctccatctt
    16441 ttttgcaaat tcaggatctt cggaataatt ggggatgcca ctggtcatat tcaataattg
    16501 tcttaatttt acatctttcc actctttgta ttgaggcagc catttaccca aaggatcatc
    16561 tagtgacaat ttgccctcag tctgcaattg aagcaaaatc aatgaagtga atgatttggt
    16621 aatgcttcca atatcaaaca aattcaatgg ggtaatttcc tgggacagtg gaggatatcc
    16681 catagttcct ttgacaaaag tcgcaacatc taatgcatcc ttatttttct cttgaggaat
    16741 aaatactgag acagcaatgg ctgtaaattt ttctttgtta tgataaacct tgaagtactt
    16801 gctcatttcc ttttgaattt taggatgaga tacaacagga gattcagctg cactgatatt
    16861 ataagataaa gaagtcaggc agaaggcaag cagaaaacca actaagttat ttattttcat
    16921 ggcgaaaaat acatcaagtt aagtagaaaa ttggccattt taacctgata gactcaaata
    16981 gatccataaa ataaaaaaaa caagagatgt tctgttgaat atacggacac gcctattcgc
    17041 attctcttcg gccaggagca atgaaatcca gcgaaattgt agttttcatt gcccacttga
    17101 aatatgaata cttctccaaa tccagatttt tttgcattca gcttaaaatc aataaatatt
    17161 tagattctaa acaacactgg ctctggctcg tattttctcc aaagcgtctt caagacgttt
    17221 gattgatttg tttttaccta acaaagccaa tgtcatgtca atggaaggag atgttccgga
    17281 tccggtcacg gcgacacgca acggttgagc gattttacct aaattcaaat caaattgcag
    17341 gctcacatca ttaatgcatt cttgaatttt atcatttttc cattcgtcaa gcgcttttag
    17401 gcgctcatgc aataccatta aaggctccag aacaactggc cgcaagtgtt ttttaacagc
    17461 atcttcatta tattcaatga tatctgtata aaaatactga ctaatttgac acatttctgc
    17521 taatgactta catctctctg cctgaactgc gaccaaatcc ttcaaatcgg gaccttgatt
    17581 caaatcaatt cctgcctgct caaaatgcca ttgcaacgca ttagcaactg attctggaga
    17641 gtcacttttt tgataatgct gattcaacca atacaactta tcataattga aacttgagac
    17701 acctcggctg acatttttca agtcaaagct ggttatcatt tcttgtacac taaaaatttc
    17761 ctgatcgcca tgagaccatc ctaaacgaac taaataattc aataaagcat gaggcaatac
    17821 acccagctcc ttgaactgca agacacttac tgcaccatgt cgttttgaca gccgtttgcc
    17881 atcttcaccc aaaatcatgg gtaaatgggc aaaaacaggt accggtgcat ccaatgcctt
    17941 gaataaatta atttgtctcg gagtattatt gatatgatca tccccgcgaa taacatgagt
    18001 tattttcata tcccaatcat cgataactac agcaaaatta taggtgggat gcccatcaga
    18061 acgaaccaaa atcaaatcat ccagttcgct attatccaca tgaatgtcac cataaacctc
    18121 atcgtggaaa gacactatac ccgcatcagg atttctaaag cgaataacat aaggaattcc
    18181 actatcagga agactttggt ttctgcaatg tccgtcatag cgtggctttt ccttggcggc
    18241 taattgagcc tctcttaaag cttccagtct ttccttactg cattgacagc gataagcctt
    18301 tccttcttcc aaaagttgct gcgctacttg ttgataacga gcatatcgct cagtctgata
    18361 ataaggacct tcatcgaagt taagccctaa ccaggccatt ccgtctaaaa tagcctgtac
    18421 agattcttga gttgaacgct ctctgtccgt atcttctata cgcagtataa attgcccatt
    18481 gtgatgtttt gcatatagcc atgaaaataa tgcagtcctt accccaccaa cgtgtaaaaa
    18541 accagtaggg cttggggcaa atcgggttct aacagtcata atatcttcca ttgtaaggaa
    18601 taaaataatc aagttataat agccgagttt accatgaacg caaagattca actgctgaat
    18661 taagactaaa aggagctatg ttgaaaagta ttggtattaa atatcagcta agaatcacta
    18721 ctcttattcc tgccttttta gtcgccttgc tttttgcttt tttttataac ggcctatttg
    18781 gtaaagatct aaagcagcat atgtccagac tgggtgaagc ctatattagg caattacttc
    18841 cagcagccca gtatgccatg cttcgcaatg attaccgtac attgcagggt ttaatcaatg
    18901 cctcaaccat taaccctgag gtaaaagctt tggcatttta taatgctgat ggtcgattaa
    18961 tcgcttatcg tggtggcaag cactctattc ataaaccatt caatccacct gactatacag
    19021 gtgactacat tgaaagtaag caaattaatc cattcaccat taattttata gcgccgatta
    19081 ctatcccaaa gttcaatttg tactccagca cagaattcaa agagctttct acgcctaaaa
    19141 tattccaggc tgacgatata ttaggctggt tatccattga cattgataca caatcactac
    19201 ttataaaacg ctatcaaatg ctcatcgtta ccatttttat taccctattc ggtttactga
    19261 tgggtttaac aattcattac ttcttgtcta aacgaattta catgcctatt gctcgattgc
    19321 gtcgaagtat gaagcaaatt ttaagcaatg aatttgaaac tgaaattcgt gtttcaagtc
    19381 caggagaatt aggaataatt gaaaaaggtt gcgctcattt acaacgacaa tatttaaata
    19441 cagttcgtga tttgaatcac cacattgaaa tcgccacagc agatctgcaa caaagtctcg
    19501 aacttctgga agaaaaaaac attgagctat cgctggaaaa gaaaaaaact gaagaaaaaa
    19561 gtcgacaaaa atcagaattt attgccaaca tgagtcatga aattcgtacc ccaatgaatg
    19621 gcgtgattgg ttttacaaat gttttattgg aaagcaagct ggacccttta caactggact
    19681 acgttaaaac gatcaaatca tcagcgcagg atttgttaag tatcatcaat gatattctgg
    19741 atttttctaa aattgatgcg ggcaaattaa atcttgattg catcccgcta gacataagag
    19801 gttgcattga tgaggtttta tccctgacaa gccccaatgc gcacaaaaaa ggaatcgatt
    19861 taatcccgat tacagacatc aatgtgccca aaatggtatt gggtgaccct ttaagaatca
    19921 aacaaatcat cagtaatctg gtgaccaatg cagtaaaatt tacagatcat ggatacgttc
    19981 ttatacgcac taaaatcgag caagaaacag ataaagatta taccttgcta tttgccatta
    20041 cggatacggg aataggtatt tcaccagagg atcaaacgaa actgtttact gccttcaatc
    20101 aggcagacac cagcatcaca cgccgttacg gtggctcagg attaggcttg gttatatgta
    20161 aaaaactatg tgaagaaatg catgggcgta ttagtctaac cagtgaaatc aacaaaggtt
    20221 ctacttttag cgcacgcatt aaagttgaaa aacttgttgc ttatgagatt gaaaaaaatc
    20281 aaactcatcg tttcgcccat ttaaaaatca tttgctttga tgacaatccc ctccatttgg
    20341 aagcaatagg taatggcttg ggattttggg gtatagaggc tatacgcgtt gactcattta
    20401 ataaactttc ccgaacattg acaaaacata aagattgcaa aattgccttt attaacgtca
    20461 atcaaggatg tgagcgacaa gctgctgaac tcattgccaa acacaagcaa attcctttcg
    20521 tgttaatttc caaatggcct attaatgatt ttgcggcgct tggtgctcgg ggttttcttt
    20581 acaaacctat cagcatccaa aagcttcagg atctgattga atcgatagcc aatgaaaacc
    20641 aaactgagaa aaatacgaac caggaactgg atactttacg cgaacagctt cgttttcttc
    20701 atccagagat actgattgca gaagacaacc ctgtcaataa aatgctatta acatccctat
    20761 taaacaataa tgccaatata accacggttg acgatggcga aatggccgtt actgcttgtg
    20821 aggataaaaa atttgacatg attttactgg atctgcatat gccaaaactt aatggtttgg
    20881 aagcagccaa aatgatacgt caaaaatccc taatgaataa acactctcct atcgttctca
    20941 ttaccgccag tagcagtgat ctgagctcca tcgatatgaa aaaatatgga gttgattttt
    21001 gttttcaaaa acctatagat gaaaagcaat tgttaatcca aattcttcgc atcgttgata
    21061 aaaccaaaca tgctgccatt gattggcaat tatgcgttca gaaagtttca ggtaatcaag
    21121 cgcttgctga agagttcctc gccaaattta tagaagaatt atataagaat cgtgaagagt
    21181 ttattgggtt aatgcatcaa aaaaacgtaa agggcctggc tgatttggct cataaattac
    21241 atggcgcctg ttgtttttgt ggcgtgccta ttttacaaaa gagagtagct caattagaga
    21301 ggcttgcaag acgaaccgct aatgctgata atttaaccga agcctttacc gatttaatac
    21361 aaagtattga tgcagtgatt agtgaatatg aaaatcagta ctcacaataa tatttaatga
    21421 aataaggcca taaaaactct cttgtatgaa aaccaaagta aaagtctgta tcaacctaat
    21481 aaaatcattc tagttatgga gacaaaatgt cagttaaaaa tgcaatctac gcccagtctg
    21541 gtggtgtgac cgctgtaatt aatgcatccg cttgtggtgt catccaaacc gccaaactat
    21601 ttccagagca aattggtaaa gtttatgctg caaaaaatgg cattatcggt gccttaaacg
    21661 aggaattaat cgatacttca ttggaaagtg aagaagacat tgccaaattg atgcaaactc
    21721 cttccggagc ttttggctcc tgccgctaca aattaaaaga caatggtgaa gagtatcaac
    21781 gcttaatcgc tgtcttcaag gcccacaaca taggctattt cttttacaat ggcggcgggg
    21841 actcacaaga tactgcccat aaagtatccc agttaggtaa aaccatgggc tatcctatta
    21901 cctgcatagg tatccctaaa acagtagata acgatctgcc ttttacagac aattgcccgg
    21961 ggtttggatc agtcgcgaaa tacattgcca tttcaacgaa ggaagctggt tttgatgtcg
    22021 cgtccatggc ggcttcatcg acgaaagtgt ttattcttga ggtgatggga aggcatgcag
    22081 gctggatagc tgcggctagt gggctggccg agcaaaatcc aggcgaaccg ccacacatta
    22141 ttctattacc tgaggttcct tttgagccaa ctcgttttct aaaaaaagtg aaggactgtg
    22201 tcaattcata tggttattgt gtgattgttg tttcagaagg tatccgcaat aaggaaggaa
    22261 aatttctcag tgaagcagga ttacgtgatg cattcggaca tgctcaatta ggtggtgttg
    22321 cacctgtcat cgctcaatta attaaatctg aactgggttt taaatatcat tgggcagttg
    22381 cagattatct tcaacgggca gccaggcaca tagcctcgca aactgatttg gatcaagctt
    22441 acgctttagg taaagctgct gtggaatatg ccgttaaagg atataacgcg attatgcctg
    22501 ttgtcattcg tgagcaggac gaaccctatc aatggtctat aggccaagtc cctctggctg
    22561 aggtagctaa ccaggaaaag gctatgccgc cagagtatat agctgaagat ggcatgggaa
    22621 ttacggacgc atgcaaacgt tacttgtccc ctttgattca aggtgaagct tatccaaatt
    22681 acaaaaatgg attgccagat tatgtccgac taaaaaacca gttagtggct aaaaaaatca
    22741 accaagatta agggcggcag taggctggac gatatttact aatcagggta ccaccagtgc
    22801 aatcccaagt gagtattctg ttagtggcat ctattgtggg aaccattgta atggtaccat
    22861 tacctgcgtt tgcagtgtag gtaatggtaa taatgccttg attaccaatg gctatagagg
    22921 ctacattaat tgttggggca ggactggtat agcccgtatc tgcctgattt gctggcaaag
    22981 tgttatcaat taaactcgtt tcaacaacag ctaatttagc ggccgaagcc atttccaatc
    23041 cttcgaccac ccgtgctctg atggtatagt cctgataggc tggaattgcg atggcaacca
    23101 gaatcccaag gatagcggca acgatcatca gctcgattaa agtaaatcct ttcattctca
    23161 taacgtcacc tggcattgca tctaactgat atcacgcaaa ttcaatgcca actctatgat
    23221 ttaaaaatcc ttctgctgac taaatagctt gacaagcttc tttcaaaaca tttctgttag
    23281 gtaaataagt cattggtaaa acatgggcaa ataaaaaacc gccattgaaa ggcggttttt
    23341 atagtggctt tatcctatta tggtctgcaa ctggcaggtc ggtatttggc caataatgta
    23401 ccacccgtac aagtccaggt tacatcaccg ttagcttgta aggttggggt cataactatg
    23461 gtaccaccac ctgctgaagc ggtatatgtt atggtaatca caccgttggc accaatagca
    23521 attgattgta cattgggcgt agctgcggga gacacgtagc ccgttgcagc ctgagtaggt
    23581 ggtaacgcgt tgttggtaat agccgtttca gatacggcaa gttttgcaga atctgccaag
    23641 gtcagacctt ccgtaacacg agctcgtatt gtgtaatctt gataggctgg tatagcgatt
    23701 gctgccaaaa taccgataat cgctacgact atcatcaatt caattaaagt aaaacccttt
    23761 tgtctcatga ccatctccat agttaactat ccattagtat aatgcaagca taatgccaac
    23821 taccagccaa tattctaaac ctgtattatt ttacatgtta aataatcatt tcatgtaaaa
    23881 aacacctgtt tttgtgctaa aaattcagtg aaatgaatag ataaaatatt tcataaaata
    23941 aactatttgt tattatataa taataaaatg acaatttttg tcacattctg acaattgtgt
    24001 ttaattaatg tgacaactat gcttcaaatg tatatactcc ttattttgca aaaacagagt
    24061 cgagcaaatc atgacgcgta aactccgcat agaagaaaaa ctaatctccg cattatcccc
    24121 cctcttttta agtgttgaag acgagtccat gaaccatcac gttcctcaag gcgctgaaac
    24181 acattttaaa ataatcgccg tttcaaatca ttttaatgat ctgactcgta ttaatcgtca
    24241 taaattcgtt aatcagcttc ttaaagaaga atttactctg gggctgcatg cattaagcat
    24301 gcatttatat acttctgatg aatggatgac tcaacagcaa tcggtactca aatcacctgc
    24361 ttgcagagat ggctataaaa acaaataatt ttaatgaaaa aagttcacct ccaagcttga
    24421 tttattttat aatgtctatc tttttatcaa atagaaaaat caatgaaatc atggtcctct
    24481 gaaaaaatat ccgttcttgc tttagtgctg ctcattaccg gagcaattga cagcattaga
    24541 aatttgccag gcacagcact ttttggatct actttgattt ttttctttat tttttcagca
    24601 attatctttt tgatcccggt tgctttagtc gctgctgaac tttcctcaac ctggtcagac
    24661 gaggaaggtg gcatttacag ttgggtcaag catgcctttg gtgaaaacat cgcctttttc
    24721 gccatttggc tgcaatggat aaatactctg gtatggtatc cgactatttt gtcttttata
    24781 gcaggcacaa tctcttattt aattaatcca gaacttgcac aaaacaaata ctatcttatc
    24841 agcgttattc tgattatttt ctggtcatta actttgatag gcttatcagg actgcgtgct
    24901 tctgccacct ttgctggctt ctgtgcaata gttggaatga tagtgcctat gggatttatc
    24961 atccttctgg cttttatttg gttaattcat ggccacccta ttgccattga tctgagcctc
    25021 tccaatttgc taccgcaatg gaaaaacaca caatcctggg tttctctaac agcgattatg
    25081 acttcttttc tcgggatgga gcttgctgca gttcacgtgc gtaatgtcaa taacccgcag
    25141 aagaattttc ccaaagcgat gtttttttcg gtcattctca tcctgttcac catgatttta
    25201 ggctcactgg ccattgcttt cattctgcct aaagacaaaa tcagtttggt agatggggtt
    25261 atgagggcat ttagtaattt cttgcaagtt tatgatttga attggctaat gcctgtatta
    25321 atactcttgt tattgctggg taccttaggc agcatgatca actggattat ttctccagcc
    25381 aagggcctgc tgatggcagc caaacatgat tttctgcctg cttcgctatg tcatgtgaat
    25441 aaacatggca tgccagcaaa aattttgata ctgcaagcca ttctggtgac agtattatgt
    25501 agcggctttc tcttattccc tacagtgaat gctatttatt ggttgtttac tgctcttagt
    25561 acagagctgt atatgatgat gtatgtgatg atgtttattg cagcctggca tttgaagagt
    25621 aaattctcag acatggaaag accttttgct attccaggag gcaaacccgg ttattacctg
    25681 acatgcctgt taggattaat aggttgtact gtcactttat gggtaggttt tattcctcca
    25741 acggaaagca ttaatatcgg aggaacagcc cattaccgta tggtcttttc attaggcatc
    25801 gtcttaatgc tgctacctgc tgccttgatc tatttgcgta aacgccacat taccaaatcc
    25861 aaagcgctta atgcacctgt ttaaatcatc gataggatgg ttgtaacccg tggcttgtcc
    25921 acagggtcag tgttttagcg gagagctcct ggatcccgtg gacaagccac gggacgtcga
    25981 agatcagtac agtactgggt tatcagagat ggatttaatt aaaatagggc ttcaatgttt
    26041 gttgtacatc gagatttaat tcgccagtat tcggattgat agcactaacc gtatcaccta
    26101 aataccctat cgcccagaaa tataaattat tggacccgat gccactttgg gtaagataag
    26161 gcatcaatgc ctgggtaaaa aagttttttc cttcaaaaga tttccaggag gcttccccta
    26221 aaacaatagg atacccagct ttgtacagga aaccccaact ccaatcgaaa cgcagattac
    26281 ttgcatcacc aggggctgtt tcccaaccag ccacttcctt tgggtaaaca tgaggtgaga
    26341 aaaccagatg gccattattt cccatattat catcacccaa cagccaaccg agtgtttctg
    26401 tgtcaatacc cggaacagcc tgttgcaatt cattactaaa tttttgtaca tcaaattgcg
    26461 ctatgttttg tttagccaaa ttcttatcca gaaggggttt aaaatcttcc ccccagttcc
    26521 ctttgaatgc tacaacctcc tgttcattgc cgcactgccc tggatcgcgc cagtgtgcat
    26581 agcccgaatc ctctttaata ttggcttttg gcacacaaat tgttggatta tccatatcat
    26641 catttccgcc tccaggtcct tcaacaaaca gtaacaaatc aggattgacc tgatgcactg
    26701 ccttggcagc agtagctatc actgtaatcc acgaaacctg accatttctg gctttaaacc
    26761 agctcaattg atagggttca ttaaaaacat caatgccaat gacattatcc aaaccacgtt
    26821 gtttgatttc ctgagcaagc agaactaaat ccttctcata ttgaaccatg ctataaacag
    26881 tgccatccct cattccatcg ccataaccat aacgatggtg aaaatcaatc atcacattga
    26941 tattatgttt tttcatttca gcaagtaccg tccagaaagc ctgcttcgga ctcactgctt
    27001 tttcacacga ttggccattg gactgccagg tcttgcaaaa aagaccatta ccggcttcag
    27061 gttgtgtttt gtcagaaagc catttgttga gatcgacttc cccactgtca tcataaagaa
    27121 cacctggctg cataggcaaa cgtacggtct tgaactctac cgctgtgctg ctgtttacac
    27181 cagagtctgc catagaccag gggtgaacca ataaatccat taatccattc cgaggtttgg
    27241 attgaggcat ggtgatatta tagaaaggat tgctctgcaa tccctgaaaa acattggtat
    27301 cctgaaatcc tgaccaggat actccattga tggtcaccct attcccactt ttgtcataga
    27361 tactgccttg actagtataa taggcattcg catccagggt gaatacgaac agcaaaagcc
    27421 aactgctaaa cttaaatatt ttcatgcgct tttcctcatt aaaataacca taaagttatt
    27481 gttaagttgg ttaaaagctc attatttaac cataaaacta caatatttga acagtataat
    27541 cattaatttt tcaaaaatat tacaaaaaga tgtcatttcg atgtgctaaa aaatagtcaa
    27601 gatcgtcact ccattatgat gaaaattgag tggcgaccat acggagtgaa taaaatcatt
    27661 ctcatagaaa aaacaataaa ttacaacgag ttatacattg aaagtatcaa gaaaatattg
    27721 ctgagccaaa atcaaatctt cttttgtatt aatatcctgt aaaggttctt cgcaggcctc
    27781 atcaacccgg atagaaaaac ctgaccatag gatgcgcaat tgctccaaac actctatttg
    27841 ttccaaggtg cagggcggcc agctaacaaa ttccagtaaa aaggcagcac gataagcata
    27901 taaaccgata tgcctgaagg tattggaata agcgttttta tcatctctgt gaaaaggtat
    27961 cgcgcttctt gaaaaataaa gagcgtgatt atctcgagtg cataccactt taaccacatt
    28021 agggttattt aatatttcaa ggctgctaat aggccagcat aaagtagaca caggcgcctt
    28081 tgtctttgtt aaagagcaag caacttgttg aattaatttg ggtcggataa atggctcatc
    28141 accttgcaca ttgacaatga cgtcatcagg agcaaagcta cctttggcga taacctccgc
    28201 aattcgatca gttccagttt ggtgcgtatg tgaagtgatt acaacctccg ctccaaattg
    28261 gattgcgcgg tcagctatct catcatgatc agtggcaata atcaccgact ttggttctgc
    28321 cagaagtgct tgcctgtaaa ctcgttcaat cacggtaata ccgttaattt cctgaagtaa
    28381 tttacctgga aaacgactgg aatgatatct ggctggtata atcacatgaa aattatggct
    28441 cataatttat ctttctcttc cagaggaatc aaacgcgctt cttgttccag cattacagga
    28501 atatcatcac gaacaggaaa tgccagccta tcaaatttgc agatcagttc ctgttttttg
    28561 aacagcaatt ttcctttaca taaaggacaa accaatattt ccaataattt tttatccacg
    28621 cgaatctcct tgagtaacct gattgcgcac atacactggt tgtgcctgcg ccacagaaac
    28681 tgccttgata tttgctttct gtgccaaacg tatcatagca gacgctgtcg gaaaaacagc
    28741 taatacttca ctaatttgtg atttgatctg ttctgggaaa tccttccagt acaaatcaat
    28801 cccaactccg gccaaaataa aggattgatt ggctggcagc tgaatatctt ttaccgcgtt
    28861 aattctgtcc tgggctaaga attgatgctc caaaaagcaa ctccaataca tttcatgcat
    28921 acgagcatcc aaaacagaca atacaggctg attaaaatcc tcttttattt cccgagctgt
    28981 ccaggcaatt gcggctaaac tgctcacagg aacaaggggc aaatcattgg cataagccaa
    29041 cccttttgca atgctacacg cgattctcaa tcctgtaaaa cttccagggc cacatccaaa
    29101 aattattcca tccaattggt taagacccaa tccggtttgc gctatcaact catcaatcat
    29161 aggtaaaatc aattgggcat ggattctttg actatcctgc tccctgctta tgatttcgtc
    29221 acctataagg atggcaacac tggccagttc agttgaggta tcaatcgcta acagtttcat
    29281 catttaaagc caactcttta ataaaatgca gtactttttc ttcatcccga gttttcggaa
    29341 gaccaggtaa acttgccaaa atggttcgac catactctct accagtcaaa cgtggatccg
    29401 ctatcattaa tataccttta tcggtgatgt cacgaatcaa tcgccctact ccctgtttta
    29461 atgcaatgac agcatttggc aaggataatt catcaaaacc ggaaagccct tttttcttta
    29521 aataagacat tctgccacga attacaggat caacggggtt agcaaagggt aatttatcta
    29581 taatcacgca agaaagcgcc tcccctttta catcaacacc ttcccaaaaa gtagaagtgc
    29641 ctaaaagcac agcattgccc aattgcctga atcgagacag gagaatgggt tttgcttcat
    29701 caccttgaat caaaagcgga tattttaaag tattgctcat catttgagca accaatttta
    29761 ctgctttatg actggtaaaa aggaaaaagc aacggccacc gcaagcttct ataactggca
    29821 aagccttttc cagcaaagct tcataataat gagcatcttt aggatcgggt attcctcgag
    29881 gcaaataaag cagagcctgt tgctgataat caaatggact ggataataac aaagtttgta
    29941 ctttatctaa tcccagaggt ttacaaaaac tatcaaaaac cgatgccata gtcagtgtag
    30001 ctgatgtaaa aatataggca catgattggt cttttaataa ttgactaaat gattcagcta
    30061 catcatacgg ggtggcatga aaaacaagag tatgtttaaa acgttctatc cagcgaattt
    30121 tatgattatt ttcctcagtg aaagaaagca agaccgtttc caaatccaaa aggcgctctt
    30181 tacatctttt taacccggga atatcagcag tatcctttga ttccaggcat tgtatcaact
    30241 catcctttaa agataaccag gtattccacg cctccataaa ctccttattt cgtttaacca
    30301 aatcccaact gattcgttcc tcacgagtcg gcaaagcatt gagcaattga tccaccagtt
    30361 tgtccgcttt atgacttata gactttagag gttgattggc taaatccagg attggccact
    30421 cattaagcat atcatcaacc aagtctttga attgacgtgt tcccaggcgc tccccattga
    30481 aattagaggc gatgtctgca agctgatgag cttcgtcaaa cactacgaca tcaacaccag
    30541 gtaatagttc gctaaaacca tcttttttta actgagaatc agcaaaaaaa agatggtgat
    30601 tgataacgac aatatctgcc tccatcgctc gcttacgtgc tttcactaaa aaacaggttt
    30661 catgattcgg acattctgca ccaagacagt tatcggtagt ggatgtgaca taatgccata
    30721 ctgaggagtc ttcattaatt tcaggtaatt ccgagcgaac gccttcttgc atttgagaca
    30781 atttatctct gacatggagt atttcgtgag cgcattgggg gctttgaaat tgtccttcct
    30841 cagcatgctt ctcaactcga tattgacaaa tataattgga acgccccttt aaattctgga
    30901 ttcgtaccga taaacctaaa gctctaatca aggttggcaa atctttttga aaaagttgat
    30961 cttggagcgt tttggttgct gttgagataa gcgctttttt accacttaac aaacaaggca
    31021 ataaataagc aaaggtctta cctgttcctg ttcctgcctc agctaccaaa atcgatcgct
    31081 gaacaatagc attggctata gccactgcca aatcggcttg tggtgctctt gccacaaagc
    31141 ctggaatagc agtggatagt cttcccttct cactgagagt tcgccgacag gtttcagcga
    31201 aagtggttat ttcttccact ctttttgcaa atattgaagt ttgtccttta cttcttccca
    31261 atccttggca tcagagggag cgtccttttt agcagtaata ttaggccagg ttttggataa
    31321 ctcggcattt aattctttga attgctgttg ttcctctgtt aaatcgtctt ctgaaacgat
    31381 agcattaact ggacattcag gttcacaaag ggcacaatca atgcattcat cggggtgaat
    31441 caccaggaaa ttaggcccct cataaaaaca atctacaggg catacttcta cacaatcggt
    31501 atacttgcat cgtatacaac tttccgttac tacaaaagtc atagcaaacc ttaaaaatta
    31561 tctttaagaa atgactcata ttaaagcata aaataccctg ctgatgatag aggagcctga
    31621 tatttttata cccctgggtc ccattcatta ttcagaacca aactgaaatc ataaactcaa
    31681 aaccaacggc atcatataca ttcttactgc tcataaaatg cacttgaagt ctcgatataa
    31741 agatgttaag tttgctcaat ttaaatctat tttgttcaat ggtctaatgc actgtctgtg
    31801 taattagata tctcggagta cttcatgcct ttaaatttac cgcctaaatc atctaaaaat
    31861 acaatgcctt tggtcattgc atacaataat gcacctgaag atgacaagat tcaaaaatta
    31921 ttttatttac aaaaaatcaa ttatttactg aataaaactc aactaaatga tgatttgttc
    31981 gattggataa acgatgctga agaaggtggc tggttaaatg agctggctaa attttcaatt
    32041 aatcccaatg cctccttttt tttaaaagga atgcagtttg ccaaagccat tactgaagaa
    32101 ataaaaaata agcctgaaat taacagttct gaagtaaaca tctaccatct catgcaagag
    32161 cgtgaccaac tgttaaaaga agtagaattt gaaaaatgcg ctactcgcta tgctgaaatt
    32221 aattttctcc tgaatgagtt agcccttaac gataaaaaaa caaaagagat tgtagaaaga
    32281 caaacagaaa tccttaggct tgtcgctccc aaaataaaag ccattaaggg agaatccata
    32341 gacaatttac cagttatacc ttcttataaa accaaagaac ttggcaatca tgtaaataat
    32401 tttaacttca aattcacaat gagcggatgg gaggctcctt ttgtatttcg tgtagaagac
    32461 aggcatgagt taggaaagga acaagaacta cactcctacg gagtctcaaa gtattttatt
    32521 gaagattact ctgtttttat gatgagattt aaagcggaag atggttctac tgtttacaaa
    32581 ccagttatct taagccaatt cgccaatcaa aataatctgg aagaaattgc caaacagtta
    32641 aaagatggca gtccaaaaaa tattgcaccg cgaattgggt attactttgt ccagttaacc
    32701 gatttttgtc taaagttaat agagactcat aactatcatc ccgacattaa gttaaataat
    32761 tttctggttc acaataacag agtactggtg agcgatagaa aaacattcac cacgaatgac
    32821 aatcctttag ctagtgaaat tctcaccagt cctttatttg ctcccgatga atttttaaaa
    32881 tgtctactat ttaacaaaga aggcgaccct gtaggctata atcgcaatgc tctgtggaaa
    32941 cgcatgaaca tgccccaatt catggcctat caactgggaa tggctttaaa acaattcctg
    33001 attttaactc aattggatga attaccagat gattttagaa atccggatca cagcgccgta
    33061 tcacatttca aaacaccatc cagacaaatc atcaatcttt cccttctggt tcaggaatta
    33121 acacgcctcg atcctgataa gcgaatgacc attaaacaat tccagacctt gcttaacttc
    33181 aaaaacttac cgcctgatgc cttttaccag aaagtggaag aagttttccc ctcctctcaa
    33241 ttagggattg cagaagatat tgaggctctt aataaagtat tgaacagcga tttaaaaggc
    33301 gaagcgcttc tgaaacaagc taatcctgtt ttcaccaaac tatcaaaata tgatcccaaa
    33361 gagacccgat taacccgcct agccgaaaaa ttggcaattc gatgttttaa caacgtgtca
    33421 aaaccttatt ttcagaaact gtctggtttg attgaatcag ccttagaaaa agatgttaat
    33481 gaaatcaggc agctattaaa tcctcaatca aatagcgaga ggcttttaca gaaagccagt
    33541 cccattttta tgaaactttt tgaaagacag cctgaggtac cccaattaac agacttggcg
    33601 aaacaattag cggctcaatg ttttgatgaa tcttcaaaaa actattttac tcgtcaactg
    33661 cctttattga ttgaagagga attgttaaac caggattggg aacaagcccc ctggtataga
    33721 aaagccctgc attggttaac ctttggatac ttcagagtcg ataacgtcac agaaatatcg
    33781 tccttgaaaa tacccgagca tacaaaaggt aaagaatttc agatttattt ttggcaactc
    33841 ttatttttac cctctgaaga aatcaaaaat atgtttacct ccaaagacag ccaaatatct
    33901 gaatttcttc tgggttatat cgaaaaggaa agagaaaaaa gagaaacgga aactccttca
    33961 gtccaaattc ctgttgattt aaaaggagaa gaatttcaga tacacttccc gcagcttgaa
    34021 tttttacctt ccaaagattt tgaaagcatt ggtgaaaaag agggtgagca tttggagtgt
    34081 tttatctttg ccaatcttca agaaatactc tcgcataata acagcgattc atcagaaaaa
    34141 aatagtacta gcgaaccctc tgatagcgaa agtgaaatcc atttaaacgc gggtaacgtt
    34201 gatacaatta taattgcaaa cgaaccaaat cccaccgacg cgaaaccgca gaaaaaagac
    34261 aatgatgggg caaaacaaaa taatccggcc aaggccagta gtcctgaagg aatagtatca
    34321 actccagagg ttgaagagca tcctaaaaag aagataaccg atgatgcccc caagccaaaa
    34381 acaaaacgtt ctgtacactt ctttgatgag gctaaaaaat ccaaacccaa ggagaaagaa
    34441 aatgcctctc ctgctgttga cgaaacgccc cccgcaaaga gaaaaaatgt ctgcaggata
    34501 gatagcgtca ggtccaccct tttcagagga gatggcagcc ataggcagaa aatcaaagaa
    34561 caaagaccca ggttatctga aatagcttgg gagccgccaa aacctaatct acaatagcaa
    34621 acagaggctt gcttcttgag gctttctgca gccaccctga ttatacaggg tggctaactt
    34681 aacaaatgtc atggtgggct tgaaacttta ttttctcttt ttgttaaaaa attgcacatg
    34741 ttttttaaca acagcatcaa actatgtcta tatttataga aaagaatata caaaaatcag
    34801 agtcaaaata atgacttatt ttttactgtg aagtaagtaa gaataactat aaatttgaag
    34861 gaaaacattg aataggaaaa acatttttat aaatatggaa ataagtctga ttgatactat
    34921 aaggaaatag gcaatggacg ttggccaaaa gataaggatt ttaaaacgtt atttaaaaga
    34981 aaaaagtatc acgccagagg acgtcaatct caatgatcag gatctaacgg cctatgccgg
    35041 gctggatatg ctattggatt atatttctat acatcacaat ggattgtttg acttcgcaac
    35101 attggaatct gatgaaaagc aatggaaaca atatgtggat atacttaact attccgcaac
    35161 ctactcacca ggctctcccc ctgctttaat cgataccagt gaacgcaaag ctcaatttgg
    35221 tttgcaatat tggctaaata cgagaacgat tgaagcactg ccaagagaca actattatac
    35281 tcgtacagtc accttatccg atgaagcaga tgaagaattt gatcagctga ttacagaaag
    35341 cagagccaaa ttgtatgaga tagcgcaaac ttttccagaa atcagctctt ttaatccagc
    35401 aaatattatc caggtaacca accaaatctc ttcgattaaa gaaaaactgg atactttgac
    35461 aggcaataat tctcaaataa cccttttaca aaaaaaactc aacgtactaa cagatttaaa
    35521 caaaattaat caactcgaga ggctcaatat tccccaggac aatggttcta acttgagtta
    35581 ttgtacgcta gcagagacta ttgaaggagt tactgagaaa atacctttaa taacacttgc
    35641 caaagtgaaa gagcaattga gacatctttg cgctcaaaat gagcttgatt tggatagtta
    35701 tagtgagtcc agagaacaat attataaact tggtatagaa ggtatactca aattacccaa
    35761 gcatggcgac ataaaagaag tacaacgtga agccatagcc cttaatatct caagattaat
    35821 gggtctagac actacttctt caacaacagt aagctataac ggtcatcccg ccttgtttat
    35881 tccttttgac aacatcaatc tattaaatga attttcttca ggtaaaattt ttaaagtagg
    35941 catggggtta agcgggcaaa gttataccca ttactccaca attaaaccat taggtgaagg
    36001 aatccaggca gatctttata tcaaggattt tggccatgct ctggcattac tttatttatg
    36061 ttgtgatacg gatgctatag gtgggtattg taaaaacaaa gcattaagaa actacagtaa
    36121 cctctatatt tttgatcagg tcattacgga cactgataaa tttatccttg attcacgaat
    36181 tagtcttcaa cccgatcagt tcattatgaa acatacccgt catgggcaag ggagaaacag
    36241 aaccttaatc gaggattctt ccctggttac caaatacgca agccttatgc gacttaaaga
    36301 gttaaaacgc attattaccc aatactgcga tcatatttca tggctgcatc ataatcgtac
    36361 tcaagccatt aaaaaacaat tgaaagaaga acttgcaaaa gacgaacgcg tacgattaac
    36421 agaagagtta aatgatgttg aaattttgga aaaagatgct gagcaaataa aaaaccaaat
    36481 tctaattcgc atcaataaaa tagccgaagt gctacctcaa acgactgggc ccgttggctc
    36541 cgatgtcatc cggcaatgtt taattctgga gaaattatta cacaatccgg ttctctactc
    36601 tgatgatgga agaccttata aacatccatg gacgacacga caagcgaata cagtacaaac
    36661 gattaatgat ttacaaaatg ggaagataca aattactttt aaattcaata ttccagaaga
    36721 aatggtcgac tttatcaaac gccatggtgg tggagactcc ttgactatta gctctcctaa
    36781 agtgataacg attagcaaag cccatctgga tagccttagg gaaaatatgt tgcatcctga
    36841 acattcacct gaattgtctc ttaatactga ttatttagac gataaagacc tctgtattat
    36901 aaaaatggcc tatcgtatag gtaatagaac caaaatttta aatgctattg cagaataccg
    36961 cgtccttatg aataatgaac aggtaagctt ccataacaaa atagaatgtc ttgcccaaac
    37021 tgaagcgaaa atcaaagcgc tgatagaatt ggcaaatgac aaaggcttgg ggatgcacat
    37081 tcttaaaaaa ttctattttg atgcgcagca acaactccaa aagttaatta atccagaagt
    37141 gttgccccat gacattggtc aagcctttgc cgcagctctt agactggaca gagtatcaga
    37201 attcaacaag gttgtgagag aagctattgt ccaaaataaa gtgaccgacc ctcaatttac
    37261 aatttttttg acaagctgta tcaaaaacga atctcttgcc aaaaattata aagacgcaca
    37321 aaaaacaagc agagccttat taatcgagtc tcagcgaatt ataaatcatc taaaatcgcc
    37381 tataattcct ttaattgttc aattaggagg gcaattgcat tcacaggctg aattagatac
    37441 ggatgggtta gccaatcttg aaaatgacct acagcttgat tttgagaaac cagatacaga
    37501 atcaatgacc actatggatt tacatcaggc tgttacgaaa gaagaaatga ttgaaaatgg
    37561 aatgacagta aaagtttaat agatttcttt taaaactatc tgtaatgcag caaatgaaaa
    37621 gagagatgtt taaacttgga ataatggagt gactatgcca gtacatgaaa ggcgccaaca
    37681 ttttcgtatt gatgatcata tctattttga ttatcgaatt cttgcaccgg gtgaattttg
    37741 ttccgatata gaaatcacta atcagttact aggtgaaaat gggcaaaagt atatggaagc
    37801 agcacaatat tttcagggaa ttgattatga gcttgccgag ctaactcagg taatggcagt
    37861 gaaagaacct gctttggcgc attaccttaa tcttttgaat gccaaaattg attatttatc
    37921 tcgacaaatg ttaatgtcaa gtaaagttca actacgtaaa gtcaacatca gcctgggcgg
    37981 catggcattt aaaacaatgg agcagattag agaaaaaacc aatctaaaaa tcattctgta
    38041 cacaaaaccc aaaatgatac ccattattgt tgatgctgta gtggtttata gtcaatatca
    38101 aagtgagacg cattaccgaa cagcggtggc tttctgtaat ttgaccagtg agcaagagca
    38161 gttgctttca caacatattt tacaagccca gataaaaagc cgtgctgact aagatggaga
    38221 tgctgtagat tggaactgtt ttttctagga taatgagcct tcaaattaaa ataacgaggt
    38281 tatatccatg agcagaacag tattttgctg caaattaaag caagaagcag aaggcttgga
    38341 aaaacaaccc ttccctggtg aattgggtaa gaaagttttt aatgaagtat caaagcaagc
    38401 atggaatatg tggctttccc atcaaaccat gttaatcaac gaataccgcc tgaatttga
//
LOCUS       DACTJE010000011        38185 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.011, whole genome shotgun sequence.
ACCESSION   DACTJE010000011 DACTJE010000000
VERSION     DACTJE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 38185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38185
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.011"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            30..935
                     /gene="glyQ"
                     /locus_tag="I9760_000401"
     CDS             30..935
                     /gene="glyQ"
                     /locus_tag="I9760_000401"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAT5042196.1"
                     /translation="MTKPTTFQEIILTLQKYWSDLGCVILQPLDLEVGAGTFHPATFL
                     RAIGPEPWSAAYVQPSRRPTDGRYGENPNRVQHYYQFQVVLKPSPLDIQDRYLDSLRA
                     LGVDPLLDDIRFVEDNWESPTLGAWGLGWEVWQNGMEVSQFTYFQQVGGLVCKPITGE
                     LTYGLERIAMFVLGVDNLFDLPWCKTANGIVTYGDVFHQNEVEMSAYNFEHANVEELF
                     KQFDYYEGEAQKLIALQLPLPAYEMVIKASHTFNLLDARKAISVTERQRYILRVRHLS
                     RAVAQAYYQAREALGFPMQKVCNGQ"
     gene            925..2991
                     /locus_tag="I9760_000402"
     CDS             925..2991
                     /locus_tag="I9760_000402"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAT5042197.1"
                     /translation="MVNDFIFELGCEELPSGAVWPLANEFSTRLLAALDKAQLSYGEV
                     KRFATPRRLAIVIHDLQTEQESQKVMRRGPAAIAAYDSEGNPTPALLGFAKSCSVTVD
                     ALTKIQTDKGEWIVFETQSEGTKTKDLLPGLVSQSLESLPIAKPMRWGEGDDEFARPV
                     HWAVMLYGSETLNHKILGVTSGCHSRGHRFHHPQEIRINSAASYEDQMKNAFVIADFA
                     TRRQAIIHQVQELAAPLGASVIMPEELVDEVTSIVEWPQALIANFEQEFLEVPAEALI
                     ASMQSHQKCFALKNMHGELLPYFITVSNIASSNPKQVVLGNEKVMRARLSDAAFFFKQ
                     DKKQPLSAHIPSTEQVIFQVKLGSMYDKVQRIKAMMDHLSQSLNLPSHLAERATLLSK
                     CDLMTGMVGEFPELQGIMGYYYALNDGEEAIVATALNEQYMPRFSGDNLPSTDLGKAL
                     SLADRMDTLVGIFAIGQKPTGVKDPFKLRRHALSVVRLLISISAPLNLSTLIKEALAN
                     YGDRLPQDKNLVAELKPFILERLQSYYQNQGISADLVHAVRARQDDWLYDLDKRLFAL
                     KSFITMPEATSLSAACKRVGNILAQAAYADKKVDIKEELIEEGAEKALFDHLNKITKT
                     VETLYIAGDYQALLKLLASLKEPVDAFFDHVMVMVEEESLKQNRLAHLQRLQELLQGV
                     ADISML"
     gene            2997..3527
                     /gene="gmhB"
                     /locus_tag="I9760_000403"
     CDS             2997..3527
                     /gene="gmhB"
                     /locus_tag="I9760_000403"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAT5042198.1"
                     /translation="MNRIILLDRDGVINTDSINYIKNVDELIIIPDSIIAIARLSAAG
                     YKIGVATNQSGVSRGLYSEDDLSAIHCKLREVLAEAGGQIDALEYCIHLPEDNCFCRK
                     PQPGMLKALAEKLNCSLDSVPFVGDRVSDIQAALAVGAQPIIVLSNMTDMSELAQYPK
                     VPIYNSLLQYVNQLLL"
     gene            3530..4261
                     /locus_tag="I9760_000404"
     CDS             3530..4261
                     /locus_tag="I9760_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal RNA small subunit methyltransferase J"
                     /protein_id="HAT5042199.1"
                     /translation="MNRIKAVGYENNELKEKAQLLADQLNLQLDQNADTCLFVTPEKL
                     TLKIRNFSLMFADFSAMTWSKRRGEGKRQGLIRACKPTKGIKILDATAGWGKDAAILA
                     TFGADVVMLERHPVMAALLADALSRRNEADIQKMCLSLIASDAISFLHSLQEKDYPDI
                     IYIDPMHPERNKSALVKKEMQVLQQLIGTDHDAMELIELSLSHVKSRVVVKWPQKVKP
                     LLPPDASIDGKTVRFDIYMAQFSSN"
     gene            complement(4750..6168)
                     /gene="ceg25"
                     /locus_tag="I9760_000405"
     CDS             complement(4750..6168)
                     /gene="ceg25"
                     /locus_tag="I9760_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg25"
                     /protein_id="HAT5042200.1"
                     /translation="MAFYNSIPLIGKHLKSESRAKKYTSEEFKRLEREYRDHIGLPEN
                     SSFSSDAFDKLVSSRVDDFRAFPKTNLPGGLLFHDKLKEANTTEGDNIRDVLSKTVLA
                     PTEKFKTAQKNFTESTSTFKELTKLIPSKFRAQDLVGTMKELTDDARKAIIEQQAHEI
                     QMLKEKLDPQKEGQAGNYVSDFKKALGLKDNDEVKKVTEGLIKELEETHKKQLGEFDK
                     STSDSLNQLHKASASESSEYLFLANLYENNKEMRQTLEKLYAKERQRKGLPPESTTVQ
                     VGISENKIDLNGIKLKDIPVIKSITGREIHQQPDGSFAIQMPMINPFYYQDPRQNVKT
                     DMMLIAQAIKASGYDSIEMNCNFPNSEKVAMERGRQAFAACIEAGFPVDKITINVNGK
                     PMKADELFKEHPKTYQDIMSRASKISKDYEDIKAPKTSTAPVNTVVVKQEIAALRTKQ
                     QQEERAQTQPVDEQRHGVTPNN"
     gene            complement(6248..7027)
                     /locus_tag="I9760_000406"
     CDS             complement(6248..7027)
                     /locus_tag="I9760_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HAT5042201.1"
                     /translation="MRILSCLVLIISLVACSSKPAPNTLVIGTIAGPETELIETAKQV
                     AEKEYGLNIKIVEFNDYNLPNEALQDGSLDANVYQHLPYLKAAILSHGYDLQAIGRTF
                     VYPMGIYSKKYKTLSELPENGIIAVPNDPSNEMRAFLLLEKAHLITLKNTTNSGIQDI
                     ESNPKQFKFKEIDAAQLPRVLPDVDAAVINTTFALPAGLSPSKDALFTEGKDSPYANI
                     IVIRRDTEKRPQLELFVKALNSEEVKEKAKKLFGEDAIPAW"
     gene            complement(7040..7687)
                     /locus_tag="I9760_000407"
     CDS             complement(7040..7687)
                     /locus_tag="I9760_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT5042202.1"
                     /translation="MSYQMVYDLLNATGETLYMVFFSTLFAVLLGLPLGILLYSSSRI
                     KPNIKLNKILSALINIFRSIPFIILLVAIIPFTRLIVGTSIGINAAIVPLTVGATPFF
                     ARLVDNVLQSLPLGLIETGYSMGANTRQIILHIILPEAQSGLIHSITVTAITLINYSA
                     MAGAVGAGGLGTLAINYGYQRFNAGIMIATVIVLIILVQLIQMVGDYLAKRCTHY"
     gene            complement(7665..8690)
                     /locus_tag="I9760_000408"
     CDS             complement(7665..8690)
                     /locus_tag="I9760_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HAT5042203.1"
                     /translation="MIELCGLKKSFSGKLALNDINLFIQEGEIFGVIGKSGAGKSTLL
                     RCINILEKPDEGEVVIDGQNLMSLSRKDLALARHKIAMIFQHFNLLNSKTVFDNIALP
                     MRIQGIDEESIKQKIEELLPVVELTDKKDAFPSQLSGGQKQRVAIARALSCSPKVLLC
                     DEATSALDPATTDAILSLLKKINELYGITIVLITHEMDVVKRICQRLSVMVDGKIVET
                     TALSNIFNKPESLARKMLYAQISPELPTCLTRRLADYATEKPLVRLFFQGEEATVPFI
                     SQTSRELNMDINILLANIDRFDGVTCGVLVVELTANPLLLEAFINRCEQAGITVEVLG
                     YVLPDGL"
     gene            9142..9558
                     /locus_tag="I9760_000409"
     CDS             9142..9558
                     /locus_tag="I9760_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK protein"
                     /protein_id="HAT5042204.1"
                     /translation="MKKMFLAVFTLCSFTAQAAVELNTFQNIVDAIAEGKRITFVINL
                     KKCTSEMPLNSAIVSVTPNAVMVIGDSRVTASDRHFTLDDPLARGTPMFDYSKFNLDS
                     EGDASIKTTVLNASSYERLGSYQMNCKLGDGFKVFG"
     gene            complement(9727..11667)
                     /locus_tag="I9760_000410"
     CDS             complement(9727..11667)
                     /locus_tag="I9760_000410"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HAT5042205.1"
                     /translation="MNDKVWTPKNPEQTRMWQFMDFAAKRHCQVFENYQDLHTWSVKH
                     PESFWETLCLFFKLNFDTPPHQILNYYSEMIEARWFSGARFNFAQKLLTRRDNHPALI
                     SLDENDCKSVISYAELYEKVAQCAAGLKAIGVATGDRVAAVMPNTSYTIIAMLATTSL
                     GAIWSSCSPDFGTQAALDRIGQIEPKVLFICDGHQYQGKKHSSSEKVVQLTAAITSLR
                     QVVICPNINEEINLEQLPNTSYWNDFIKPVKECDFISLPFDHPVYIMFSSGTTGKPKC
                     IVHGAGGTLIQHLKELGLHSDIKSADNLCFYTTCGWMMWNWTVSALALGATITLYEGS
                     PTYPTDNRMFRLIDEEKVTVFGTSAKFISAIEKAGVKPKSEFQLSELRCILSTGSPLL
                     PKNYDFVYEHIKKDLQLSSISGGTDIISCFALGNPILPVYRGELQCIGLGMEVAVYDE
                     QGHSIKQKRGELVCTKPFPSMPVCFWNDKDRQAYKHAYFERFPGVWAHGDFAEITAHN
                     GLIIHGRSDAILNPGGVRIGTAEIYRQVEKVDDVVDSIVIGQDWQDDVRVVLFVKLRE
                     GKKLDDELINLIKTTIRKNASPRHVPAKILQVPDIPKTLSGKVVELAVRQVVHGQSVS
                     NLQSLANPQALEYFKDRNELKS"
     gene            complement(11824..12732)
                     /locus_tag="I9760_000411"
     CDS             complement(11824..12732)
                     /locus_tag="I9760_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HAT5042206.1"
                     /translation="MDYPQHVTIIEVGPRDGLQNEPSFLHSDKKIELINLLSQTGLKE
                     IEVTSFVSAKAIPQLADNEEVFQSINKTPSINYSALVPNERGMLKALEMGVQNIAVFT
                     AASELFNQRNINCSIKESIERFKPVLALAKTNQIRVRGYISCVLGCPYEGYIQPSQVV
                     SVTKMLLDLGVHEISLGDTIGVGTPRQTQLLLDAILPILPITQLAMHFHDTYGQAVAN
                     IYASLEYGVNRFDSSVAGLGGCPYARGASGNVATEDVLYLMHGLGIDTGIDIFKIVAA
                     GDMICKALGRKNQSKVANAMLANPCN"
     gene            complement(12743..14707)
                     /locus_tag="I9760_000412"
     CDS             complement(12743..14707)
                     /locus_tag="I9760_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HAT5042207.1"
                     /translation="MFNKILIANRGEIACRIIKTAHSMGIQAIAVYSAADRNSLHVRL
                     ADSAYYIGEAPAKESYLNIDHIIQAAKESGAQAIHPGYGFLSENPDFAKACEQAGIVF
                     IGPSIKAMEAMASKQLAKQLLEKTKVPLTPGYHGVEQSEEKLLSEAKKIGFPVLIKAA
                     NGGGGKGMRAVHDEKEFHDALAGAKRESMASFADDTMIIERLVLNPRHVEVQIMADNH
                     GNVVNLFERDCSIQRRHQKIIEEAPAPNLLPVLRQRLAEAACEVARSINYRGAGTVEF
                     LVDGEDKFYFMEMNTRLQVEHPVTEMITGLDLVAWQIKIAANDTLPLLQNQIQAQGHA
                     IECRIYAEDPYQGFIPSIGQLQFLKEPSGDGIRIDTGVTLSSEITRYYDPMIAKLIAW
                     GHNREEALHRLERSLAHYDIGGVKTNIPFLRAICQHVKFKEAKLSTDFLEKENISLPK
                     PDNELGMLLAISYDYLGMINRTTDPLLQEAFGWQMHLSSHWIWRYQLNSTIIEAQITP
                     IDNKKFKAKIENKEMVIYARYDIDQLIIEIDQKSVKARVENKDHHLIFYTDKGQLSIE
                     RFYWSKLDAQTSAHKGQLTAPMPATVVAILKNIGEQVKAGESLIVLEAMKMEHTIHAP
                     IDGILSDIFYSVGSQVSEGAELLALSESDT"
     gene            complement(14700..15494)
                     /locus_tag="I9760_000413"
     CDS             complement(14700..15494)
                     /locus_tag="I9760_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101538.1"
                     /note="catalyzes the hydration of
                     gamma-carboxygeranoyl-CoA to
                     3-hydroxy-gamma-carboxygeranoyl-CoA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-carboxygeranoyl-CoA hydratase"
                     /protein_id="HAT5042208.1"
                     /translation="MSDLLYEIQDKVGLLTMNRISKHNAFDNQLLTEMRIRLDSAIND
                     TNVRVIVLKANGKHFSAGADLTWMQSMANFTEEENLEDSLVLGNLMYSISQSPKPTIA
                     MVQGAAFGGGAGLAAACDIAIASTSARFCFSEVKLGLIPAVISPYVVRAIGERAAKML
                     FMSAEVFDATRAYSLNLVQHCVPDDTLLEFTLKYASQISNNAPEAVKNSKQLAQYVAN
                     KKIDEELVRYTASLIAHKRVSDEGQEGLKAFLNKEIPNWNKGSGHV"
     gene            complement(15514..17121)
                     /locus_tag="I9760_000414"
     CDS             complement(15514..17121)
                     /locus_tag="I9760_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylcrotonoyl-CoA carboxylase"
                     /protein_id="HAT5042209.1"
                     /translation="MAKLTTQINTSSQEFKNNQANMQALVTDLREKIHQISLGGDEKA
                     RTKHQQQGKLLPRERLHQLLDPGSPFLELSQLAAYQVYEDTIPAAGIITGIGRVAGNE
                     CVIVVNDATVKGGTYYPLTVKKHLRAQEIALINHLPCIYLVDSGGAFLPLQDQVFADK
                     EHFGRVFYNQAQMSALNIPQIAVVMGSCTAGGAYVPAMADESIMVKNQATIFLGGPPL
                     VKAATGEVISAEELGGAEVHCRHSGVSDHYAENDAHALHLARVAISNLNRKKPDSIHR
                     VDTVPPLYDSEDLTGIIPTDPRKPFDIREIIARVVDGSEFDEFKALFGTTLVCGFARL
                     YGYPIGIIANNGILFSESAQKGSHFIELCCQRKIPLVFLQNITGFMVGSKYEASGIAK
                     HGAKMVTAVANANVPKFTIIVGGSFGAGNYAMCGRAYAPRFLWAWPNARISVMGGEQA
                     ANVLAQITREKYAKQGKEWSLEEEEQFKTQMRSQYETQGNPYYASARLWDDGVIAPQD
                     TRKILGLGLSAALNAPIEDTRFGVFRM"
     gene            complement(17131..17445)
                     /locus_tag="I9760_000415"
     CDS             complement(17131..17445)
                     /locus_tag="I9760_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042210.1"
                     /translation="MLRHSLIYLLLSILIVVFAKYAHLIVVYIDMFFTYVNLKLTPIF
                     SQTGWGLIIRKILVLMLLPIIIAGIPALIYRALKGKDMPHFIAIVWIIWTIIVLSDIL
                     IR"
     gene            complement(17583..18767)
                     /locus_tag="I9760_000416"
     CDS             complement(17583..18767)
                     /locus_tag="I9760_000416"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HAT5042211.1"
                     /translation="MEHNDVVIVAAKRTPMGAMLGNLSALSSPELGAVAHMAALSQSG
                     IAPAEIDEVISGCVLQAGIGQAPARQAAIKAGIPNSTGATTINKMCGSGMKAVMLAHD
                     LIKAGTANVILASGMESMSNAPYLLSKARAGYRLGHGELKDHMFLDGLEDAYDKGQLM
                     GCFAETTAKHFNFSREQQDEYALRSMTKALKAIENGAFREEIAPVTLTTRKGDITVDV
                     DEGPETAKLAKIPQLKPAFQADGTVTAANSSSISDGAASLILMSASNAEKRGIKPLAK
                     IIAHASHSQAPQWFTTAPVDAIRKVMNKASWKQDDVDLFEINEAFAVVAMAAITQLEL
                     NPEQVNIHGGACALGHPIGASGARILVTLMHALKHQGKKRGIASLCIGGGEATAMAIE
                     LI"
     gene            complement(18779..19948)
                     /locus_tag="I9760_000417"
     CDS             complement(18779..19948)
                     /locus_tag="I9760_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAT5042212.1"
                     /translation="MSLMYTGLDFQLGETYDMLRDSVNQFAQTEIAPLAAQIDEKNTF
                     PNHLWRKLGEMGLLGITVSEEYGGANMGYLAHAIAMEEISRASASVGLSYGAHSNLCV
                     NQIFLNGNNEQKQKYLPKLISGEYIGALAMSESNSGSDVVSMQLQARSAGDKYILDGT
                     KMWITNGPDADVLVVYAKTDKQAGSKGITAFIIEKGMPGFKTAQKLDKLGMRGSNTCE
                     LVFDQCEVPSENVLGTVNQGVKVLMSGLDYERTILAAGPVGIMQACMDVVLPYVHERK
                     QFNQAIGEFQFIQGKLADMYTDLNACRAYLYAVAKACDNGRVSRKDAAGVILYTAEKA
                     TQMALQSIQILGGNGYINEYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFKETA"
     gene            20274..20960
                     /locus_tag="I9760_000418"
     CDS             20274..20960
                     /locus_tag="I9760_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5042213.1"
                     /translation="MRKLVLWGHGIEDYREMFDLPQGVENSRLLEYGCGPSAVNYQQT
                     QVKKNGVVSCDPLFVLDKDTLLAKAKMIFANMAEEVRQHQDQFDFSRNGSLDKLLQER
                     QEGMKQFFADYEQGKADGRYLGLTDYHLPFADFTFDFALSSHYFFADLDDQTVDFHLI
                     AIRELARVAKEVRIFPLIDREGNTSQFLGPVLLGLQQENYGVEVREAAFHLHKSGNAM
                     LRVWAQQCVV"
     gene            21009..22007
                     /locus_tag="I9760_000419"
     CDS             21009..22007
                     /locus_tag="I9760_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042214.1"
                     /translation="MAVSNKKNSEFTPSLRSHMIAALLSMGAAAIILWLASGMTLAAV
                     IVDSFSLGFYIGFMLLMSAAPAVLASPVILPGLLLVVSGLFLGAVAATFAHRLKNNPS
                     LPQNNNGNGLINELTKDKSELLISHDDLQCQSNSLEGENTLSQQNEDNVTGEKFGFMK
                     FWGSLGLTSFASSGATALLGWLATDFVVSLSIIPVFLLGMTLAIVLVPLSIGLSGIFA
                     LPAVTALMFALGGFATGVFAATLAAYFIADNQAQDHPVEPIIRSSMERQCKDQGSHQK
                     ILNGLKKDKLSANEEVINSDLNEQKSRGPKTSKSDTGVKEEHINTAEEYSFCGLRF"
     gene            22293..23294
                     /locus_tag="I9760_000420"
     CDS             22293..23294
                     /locus_tag="I9760_000420"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAT5042215.1"
                     /translation="MYSLLRPLLFRLDAEKAHSLTLSLLHYLPGFYFRKIAGQPVHAM
                     GLVFPHQVGLAAGLDKNGEHLDALAKLGFSFIELGTVTPKGQAGNPKPRLFRIAEANA
                     IINRMGFNNSGVDVLVENVKRANYKGILGINIGKNKETNLNQAAEDYLYCFRKVYDHA
                     SYVTINISSPNTPDLRQLQQGDYFAELLTQLQKEQIKLADQYGRHVPLVVKVSPDETD
                     ETLKQMTDIILQYGIEGIIATNTTCSREMVKNLPCSEEQGGLSGRPLMELSTRCLRLL
                     KQYVGNDVTLIGVGGIDSLESAKDKINAGASLLQVYSGLVYKGPELIHDIVSGLNAI"
     gene            23315..24097
                     /locus_tag="I9760_000421"
     CDS             23315..24097
                     /locus_tag="I9760_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5042216.1"
                     /translation="MYITKELAVRMEGCIKQSHIEVTKRYSQGKILEINGGAACFSGF
                     ESFLSQVVGWGFKTEPNQYKAEIEAIENFYKSLGHNRVDIELCPFVGNHLINFLSQRG
                     YYITELNNVSVLDLKSYHLVAHSEDDGFIIREVKQSELEEWAKRVAIGFGFLEAQEQF
                     YQYASAKGVLAFAAYDHGNLVAGATIAIHGDACDLGVTSTLPLYRGKGLQKKLLFARL
                     NLAKQYGLEFATVTTQPGTVSDLNVQKAGFRCAYTRIKLTME"
     gene            24184..25950
                     /gene="msbA"
                     /locus_tag="I9760_000422"
     CDS             24184..25950
                     /gene="msbA"
                     /locus_tag="I9760_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HAT5042217.1"
                     /translation="MKNNLPIKSRLLYKRLLSYVKPFWPVLLLGVLANILYSGIDAGF
                     TYMTKLFLDKSFITIDLNFVKQIPLIVLIGITLRGLVSSLGSYCMTWVARSVVKVLRQ
                     TVFSHIIHLPADYYDEATSGQLLSKILYDVEQVAQVSADALTDFIQNICLVIGLLTVM
                     MVICWQLSLMFLLTIPFVGIIVNYTNKRVRRISHKVQKTMGEVTEIASEAIEGYRVVR
                     IFGGERYEITKFNKATEYSRKNDMKVAISKAINVSGVQLVIAIGIATIIMAAIHLSTV
                     ITISAGSFLAIIAAMLQLIKPMKTLTTLNATIQRGLAGAESVFNLLDLPLERNNGLIL
                     KEKIRGEIEFKHVYHAYRQGQNILHDVNFVIEAGTSVALVGHSGSGKTTIASLLPRFY
                     ELSQGMITLDGMPIQQLSLESLRKQMSLVSQNVTLFNDTLANNIAYGRFDASREQIIT
                     AAKLAYADEFIKQLPDGYDTRVGENGVLLSGGQRQRIAIARAILKDAPILILDEATSA
                     LDSESEHYIQAALEQVMKGRTTLIIAHRLSTIKHAHKIIVLQHGRIVEQGSHQELLDM
                     DGHYAQLYKVQQFGRINEEVVA"
     gene            25950..26921
                     /locus_tag="I9760_000423"
     CDS             25950..26921
                     /locus_tag="I9760_000423"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAT5042218.1"
                     /translation="MSFFVNRIWYGNHFLQWILVPFSWLYRIVIRTRRWYLQRFCQQL
                     YPIPIIVVGNVTVGGVGKTPLVIEIAKKIQQKGLKVGIVSRGYKAAIKHFPYEVKLND
                     SAELVGDEPLMMARKINCPVVIAPKRNEAVRYLLDKHSVEIIISDDGLQHYKMGRSIE
                     IVVIDGMRKLGNGFCLPAGPLREPDSRLKQVDFVIVNQGAAEGTYSMELIPKNIVRLS
                     TQEEVSNDLFTSEVAAVAGIGNPQRFYSTLSQLGIKFNPYSYPDHHQFKPHDLNDIDL
                     PVIMTEKDAVKCYSFSSDKLYYLPVEAKLNDSFWEAFWSHQQLQGYY"
     gene            26923..27873
                     /locus_tag="I9760_000424"
     CDS             26923..27873
                     /locus_tag="I9760_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042219.1"
                     /translation="MMPKRIWLLLLSMFLFLSGSVFAVEEPEEDKVAQTNEPTVGSWV
                     FTGMVTNESGDRYGYFFQMQRQGTDFYAKTALIDGQTNQLILFYEANEKIDSSTQLNW
                     QVGRSFLRYNPINDSWIFGVKVENAKGFNFKVDMLKQDNKDVETQVLRPGVELQAVQT
                     SRLNGHVQTGVDNKEQFVTGNKAWFSKLWFSKDQNSPHDISTIFCRLSNDNGFYSANL
                     KEPDATGAAVAGWRDPSGNNVKMSQFVSIKTLTDNQCLLSVGLPRLKLKLVNTIKQED
                     DFPLTVAGFSEENPKGFCFISKQTFKQIKESAVQAVTNLG"
     gene            complement(27875..29164)
                     /locus_tag="I9760_000425"
     CDS             complement(27875..29164)
                     /locus_tag="I9760_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042220.1"
                     /translation="MQIINLHKHNNAIYSINFNIIQTTLNNLSAIKKSKITIWLVGVF
                     FLLFQFFLQLSSGIIVGAIMHEEHISALSAGLLSSAFYYVYTSFQIPVGILFDRYNTR
                     YLLSVNATLCAIGCFVFASGHNLPTLFLGRLIIGAGSAFAFIGLSHLLRQHFPLKQYA
                     FMIGLSETLGFTVTVLGMIGMGSVISHFGWRYFINIAGIIGFLIASLCWLCIPNSKPV
                     VSFPHQYKQQIIPILKNKLAWFNGLFVCLEFSVITVFAAMWAVPFLQLKLDCTIKTAS
                     ILTSMVLLGAGLSCPLLGQISVHLNRRKPLIHASCLSTALLLILVLYLPTQNKLLIGI
                     LMFSMGLTCGAYMLAYSISNELAPPEFLSTCTGFTNTLAMLSAPLLQPLVGFFLDVLS
                     NHKSQYSLADYQLALLIVPFAMLLASLFTCLLPEKSN"
     gene            29307..30197
                     /locus_tag="I9760_000426"
     CDS             29307..30197
                     /locus_tag="I9760_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT5042221.1"
                     /translation="MNLRDLHYFVILADVKHFGEAAKRCFVSQPTLSMQIKKLEEELG
                     VVLFERTNKQVLLTDQGSKLLDRTRKILILIDEMKELARQSEDLFTGELRLGVIPTVS
                     PYMLPLVMPELKNEYPRLKVWLIEDQTHRLITKLEQGELDVAIMALPIDKRFSCQTLY
                     EEKFYFACANTHPLAQAKSVNINDLKNQPIMLLEEGHCLREQAMAVCQSAKADDIADF
                     TATSLETLRLMVQAGMGVTLLPALSTLTASTNHLKCIPFSEPAPSRILGLFWRAGTPR
                     QVCFNAIAELITKNVQSKLA"
     gene            complement(30245..30799)
                     /locus_tag="I9760_000427"
     CDS             complement(30245..30799)
                     /locus_tag="I9760_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042222.1"
                     /translation="MIRLLIITISVLCSGLTIAKSSYQIDLILFAQPQNGVKSIKSDS
                     PVPLIPVSPNAIQLHSDNNKKHMPYTLLPRSQSKLSDQHYLLSRKSQFQILGHYSWRQ
                     PAKSDSLVALPKINHNGWQIQGTLKVRQSNYYLFDADLQCLSPGNQETSFTVSQKQRL
                     KGSTIYYLDHPQLGMLVKIHKIAS"
     gene            complement(30915..31988)
                     /gene="apbC"
                     /locus_tag="I9760_000428"
     CDS             complement(30915..31988)
                     /gene="apbC"
                     /locus_tag="I9760_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAT5042223.1"
                     /translation="MTIEDTVIKLIGSLKDPLLDLTGKEMNLQYKITTSNQAMHVVLT
                     AGYPTSLLETSYKPIVQKIVQDEFPNYQVTISIQQFIKAHKTQLTGKALRGVKNTIAV
                     ASGKGGVGKSTVTVNLAAALAKLGARVGILDADIYGPSIPLMLGETKPVQVKDNCYIP
                     VEAHGMQAMSIGYLTDTNQALIWRGPMLAKSLIQMLDITLWNELDYLFIDLPPGTGDI
                     QLTLVQKIPLTSAIVVTTPQNVATLDAQKAITMFSRTGIDVLGIIENMSTHICSHCGH
                     QEAIFGRGGAAALCDAYQCTLLGQLPLDSHVRRHCDEGVPTATHSSNQLTDTFIKTAM
                     RTAIELSKKPINYADRFPKIVVE"
     gene            complement(32195..35425)
                     /locus_tag="I9760_000429"
     CDS             complement(32195..35425)
                     /locus_tag="I9760_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HAT5042224.1"
                     /translation="MRLIDSEQRSQATDPAESFIVQAPAGSGKTEILTQRFLRLLSTV
                     QSPEQIIALTFTRKAASEMRERIVQALHMAASNQPAVSAHQQSTLNFAKQALQRNNQY
                     QWDLLEQPNRLKIITIDSLCQSINRAIPLLEKQVAYPDISETPDSHYISAARCCIQYA
                     IATPEYQEAIKTLLLHVDNKQERLIQLFQALLSQRDQWLPTLFLAREQEKSTFEQALR
                     LIEQHELSRLRNSLPSDLAQELVLLSRELAVIENKPDSPRFILREWYEFQKCDQQIAG
                     ALSKLLLTSDNQLRKSFDHHVGLVKKNCAPNEFTRIKTASSDLFAKLQDCPDFLDALL
                     KVNQLPTPEYDKEQWDVLQALFLLLPLLVGHLQVSFGEHNEVDFTAISQQALAALGDL
                     DNPTDLALYLDHSIHHLLIDEFQDTSITQFELLTKLVQGWLPGEGKTLFLVGDPMQSI
                     YRFRQAEVGLFFQAKEHGIGPVRLKSLELRCNFRSTETIVNWVNDHFSKIFSQQVDIE
                     SGAVSFHPSVHVIEKNESSTIQAVQFKNREQEARHLIEIINSELNRNPEQTIAVLVRT
                     RTQLPEIISLLRQNNIPYQGTDIDLLANLVHLRDVWSLAHALLFPGNRLSWLSILHSP
                     YVGLGLSDIHCIAQYNSRKSIYHNLLHLDKIQDISEEGRIRANFFIQIMNQALLCRSH
                     SRLSDWIADTLKNLHVEKILDKAQLNDLEQLWMLLDQYEKEGRIPDMNEFLNEFNKLY
                     AQQTTPSRLQIMTIHKSKGLEFDTVILPGLGSQANRGESPMLRWLKLPTQDHGNLLLV
                     SPIKSANQDNCPLYDYLDQLDEEKSAYEAQRLLYVAVTRAKSRLYLLDHSAKSYKSSF
                     RNLLKYQEFIADPDEAQHEEQEFPLPKLVKLPIEYYQKQIPSLVQSQESSCPEISSGI
                     PRLIGIVSHQLLQWICDNHPQTINHVPWNLVHNELKALGLNQEAQQTALSMIKIQISQ
                     MYENKIGLWILSRHENEQNEYELLVEYENKLVTRIIDRTFTENGTQWIIDFKTGKEDI
                     NTLQKHRQQLNEYGRYLSDCTKFPIRCGLYYLANGHWIDWQYKMSA"
     gene            complement(35430..37991)
                     /locus_tag="I9760_000430"
     CDS             complement(35430..37991)
                     /locus_tag="I9760_000430"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HAT5042225.1"
                     /translation="MQQVVIKFGGTSVSTRATWNNIAAITQKHLNTGVQPVIVCSALT
                     QISNKLEKAIEAALLDEHHSLFNDIQSSHLNLAEQLEVNHQLIANDLHQLQQWLTGIS
                     LLKQAPAKTHAQILSLGELMMTRLGHAFLEKQGIKVKWYDARELLTSTPTLGGETMNY
                     LSARCESEYDSALVEKFLSSGAQAIITQGFFAANPHGETVLLGRGGSDTSAALLAGKL
                     QAASCEIWTDVPGIYTANPHQLPHARLLKQLNYDEAQEIASMGAKVLHPNCIPPVRKA
                     NIPMVVKYTHLPEHSGTLITKDIDESAPLIKSIQVKHSILLISIDTLNMWQQVGFLAD
                     VFAAFKKHGFSVDLLSSSEFNVTLSLDVNAKIHDRPAINALLDDLNQFGRAKLIEPCS
                     AVSLVGHHIRTVLPHLGPALEVFEAKQVYLMSLASNDLNLTFVVDESHADKLCQRLHH
                     LLIESNPQIFYYSKSWHEEFGKPNVRPTPWWEIERDRLLTTSAIHSPCYVYHSPTQAT
                     RARQLSALESIDSLFYAIKANPFPTILKTLEKEGIGFECVSIQELELVLKLFPNIKKE
                     RILFTPNFAPKSEYEFALQTGCYVTIDSLYPLENWPDLFKDREVIVRIDPGTGAGHHK
                     HVSTGGNESKFGITQNDISKILSLTKVNHIKVIGLHAHSGSGILSTDLWQQTAVMLAS
                     LTDQFPEVRSINLGGGLGIVEKPGQHPIDFAALDAQLMAVKSQYPGLAIWLEPGRFFV
                     AESGVILAKVTQCKEKGKVKFIGIETGMNSLIRPSLYGAYHEIVNLTRLHEEKAGFAH
                     IVGPICESGDTLGYDRLLPVTREGDVILIANTGAYGHCMSSHYNLRPPAQEIVLE"
BASE COUNT        11211 a         7101 c         7765 g        12108 t
ORIGIN      
        1 aaagatcata tttgtttttt gagattttca tgactaaacc tactactttt caggaaatta
       61 tactgacctt gcaaaaatat tggtcagatc ttggttgtgt tattttgcaa cctttggatc
      121 tggaagttgg cgcgggtacc tttcatccag ctactttttt aagagcgatt ggacctgagc
      181 catggtcagc agcttatgtt cagccatcca gaaggccgac ggacggtcgt tatggagaaa
      241 atccaaaccg ggttcagcat tattatcaat ttcaggtggt attaaaaccg tcgccattgg
      301 atatacaaga ccgatacttg gattcgttac gcgcacttgg agtagatccc ttattggatg
      361 atatccgttt tgtcgaagac aattgggaat caccaactct aggcgcatgg ggcttaggtt
      421 gggaggtttg gcaaaatgga atggaggtgt ctcagtttac ttattttcaa caagtgggcg
      481 gacttgtatg taaaccaatc actggtgaat taacctatgg tttggaacgt atcgcaatgt
      541 ttgttttagg ggtagataac ttatttgatt tgccttggtg taaaacggct aatggcatag
      601 tgacatatgg ggatgtattc catcaaaatg aagtagagat gtcagcttat aattttgaac
      661 atgccaatgt tgaagaatta tttaagcaat ttgattatta tgaaggagag gcacaaaaat
      721 tgatagcttt gcaattacct cttcctgctt acgaaatggt cataaaagct tctcacactt
      781 ttaacttact ggatgcacgt aaagcaattt ctgtgacaga gcggcaaagg tatatattac
      841 gtgtaagaca tctatcaaga gcagtagctc aagcttacta tcaagcgcgc gaggcgttag
      901 gtttccctat gcaaaaggtg tgtaatggtc aatgatttta tttttgaatt aggatgtgaa
      961 gagctgcctt ctggtgctgt ttggcctttg gcaaatgagt tttctactcg attgttagcc
     1021 gcattagata aggcgcaatt aagttatggt gaagtcaaga gatttgctac accaagacgc
     1081 ctggctattg tgattcatga cttacaaact gaacaggaaa gccaaaaagt catgcgacgc
     1141 ggtcctgctg caatagcagc atacgatagt gaaggtaacc cgacccctgc tttgttagga
     1201 tttgcaaaat catgtagtgt tacggtagat gcgctcacca agattcaaac tgacaaaggt
     1261 gaatggattg tttttgaaac tcagagtgag ggtactaaaa caaaagatct cttgcctgga
     1321 ttggttagtc aatctttaga gtctttacct atcgcaaaac ccatgagatg gggagaagga
     1381 gacgacgaat ttgcccgacc tgtccattgg gccgtgatgc tttatggaag tgagacactc
     1441 aatcataaaa ttttaggcgt tacaagtggg tgtcatagca ggggacatcg ttttcatcat
     1501 cctcaggaaa taagaataaa ttcagctgca agctatgaag atcagatgaa aaatgctttt
     1561 gtaattgccg attttgctac taggcgacaa gccattatcc atcaggtaca agaattagca
     1621 gcacctctag gtgcttcagt gattatgcct gaagaattgg ttgatgaagt cacctcaata
     1681 gtcgaatggc ctcaagcgct cattgcaaat tttgaacagg agtttttaga ggttcctgct
     1741 gaagccctta ttgcttccat gcaatctcat cagaagtgtt ttgcactaaa aaatatgcat
     1801 ggtgagctgc taccttattt tattacggta tcaaatatcg ccagttccaa tccaaaacaa
     1861 gtagttttag gaaatgagaa ggtaatgagg gcaagattaa gtgatgctgc ctttttcttt
     1921 aaacaagata agaaacagcc gctaagcgcg catatacctt ctacggagca ggttattttt
     1981 caagtaaagc tgggaagcat gtatgataag gtacaacgaa taaaggcaat gatggatcat
     2041 ttaagccagt ctctcaattt accctctcac ctggctgagc gagctacatt attaagcaag
     2101 tgtgatttga tgacaggtat ggtaggcgag tttcctgaat tgcaagggat tatgggatat
     2161 tattatgctc ttaatgatgg cgaagaagct attgttgcta ccgctttaaa tgagcaatac
     2221 atgcctagat tttctggtga taatttacca tccactgatt tgggcaaggc actttccctg
     2281 gcggatcgta tggatactct ggtgggtatt tttgcgattg gccaaaagcc aactggagta
     2341 aaagatcctt ttaaattaag acgccacgct ttgtctgtag tccgtttact catttcaata
     2401 tcagcaccgc ttaatttatc aactttgatt aaagaagcct tggctaatta tggtgatcga
     2461 ctccctcaag acaagaattt agtggctgaa ttaaaaccat ttattcttga gagacttcaa
     2521 tcttattatc aaaatcaagg tatcagcgct gatttggttc atgctgtccg tgctcgtcag
     2581 gatgattggt tatatgattt agataagcgt ctattcgctt taaagtcatt tatcactatg
     2641 ccagaggcga catcattatc agctgcttgt aagcgtgttg gcaatatctt agctcaggca
     2701 gcatatgcgg ataaaaaagt agatattaag gaagagctta tagaggaagg cgctgaaaaa
     2761 gcattatttg atcatcttaa taaaattact aaaacagtag aaactttata cattgccgga
     2821 gattatcaag cattacttaa gcttctggcc agtcttaaag agccagtaga cgcttttttt
     2881 gatcatgtga tggttatggt tgaagaggag tcattgaaac agaacaggct tgcgcacctg
     2941 cagcgattac aagagttatt gcagggtgta gctgacattt caatgttgta gcacttatga
     3001 atcgaattat attgcttgat agagatggag taattaacac tgactcaatc aattatatca
     3061 agaatgttga tgagcttatt attatacccg atagcattat tgccatagct cgtttaagtg
     3121 ctgccggata taaaatcggt gttgcaacca atcaatctgg agtttcaaga gggctgtact
     3181 ctgaggatga tttatctgcc attcattgta agttgagaga ggtacttgca gaagcagggg
     3241 ggcaaattga cgccttagag tattgtatcc atttacctga agacaactgt ttttgccgaa
     3301 aaccccagcc aggaatgcta aaggctctgg cagaaaagtt aaattgttca ctggatagcg
     3361 tgccctttgt aggggataga gtttcagata tacaagcagc attggcagtt ggtgcccagc
     3421 ctattattgt actttctaat atgactgata tgtctgagtt ggcacaatat cctaaagtac
     3481 caatttataa ttctctgtta cagtatgtca atcaattatt attgtaagaa tgaatagaat
     3541 aaaagcagtt ggttatgaaa acaatgaact caaagaaaaa gcacaattgt tagctgatca
     3601 attgaattta cagttagatc aaaatgctga tacctgcctt tttgttactc ctgaaaaatt
     3661 aactttaaaa attcgaaatt tttctcttat gtttgctgat ttcagtgcta tgacctggag
     3721 taaaagaaga ggagagggta aaagacaggg tttgatcagg gcttgcaagc caacaaaagg
     3781 gattaaaatt ttggatgcta cggcaggttg gggaaaagat gctgctattt tggcaacttt
     3841 tggtgccgat gtggttatgc tggaacgtca tccggtcatg gctgcactgc ttgctgatgc
     3901 actatctcgt agaaacgaag cggacattca aaaaatgtgt ttgtctttga ttgcaagtga
     3961 cgctatttca ttcctgcata gtttgcaaga aaaggattat cccgatatta tttacattga
     4021 tcccatgcac ccagaacgaa acaaatcggc tttggttaag aaagagatgc aagttttgca
     4081 gcaattaatt ggaacggatc atgatgcaat ggagttaatc gagttgtctt tatctcatgt
     4141 taagtcaaga gttgtagtca agtggccaca aaaggtaaaa ccattactcc cgccagatgc
     4201 ctcaattgat ggtaagactg taaggtttga catttatatg gctcaatttt cttccaacta
     4261 accaaactct tccagttctt ctcttaaccg cttttcctcc aacagatctt ctatttttct
     4321 acgtctttcc aattttgttg aagggcttgc ctcagttata acaggttcag catcactaaa
     4381 atcatccatt tcattgtcaa attcatcaaa gcgacgagta ctcatagctt tctccttagc
     4441 taacccgttg aattccatat ctattctcga tgataccaaa ataccttgaa tagttatact
     4501 ggcactttat ttttaaatgc cacctcaaat agcattattg acacagttta tccaaggtaa
     4561 acggtatctg ctttatggat acttctaata agcggcgtaa atagaatctg tcatttaaaa
     4621 ataaagtatc tacaatggat aaaatatcag aaattgatgg tttgaggtag tgaaattttt
     4681 tgagcaaatt tcggggagga taatgaattt gttgaatttg gattgtttaa tccaaattca
     4741 acttataagt caattattgg gggtcacgcc atgtcgttgt tcatcaacag gttgtgtttg
     4801 agctcgttct tcttgctgct gtttggtgcg taatgcggcg atttcctgtt tgacaactac
     4861 agtgttcact ggggcagttg atgttttggg cgctttgatg tcctcgtaat ctttcgagat
     4921 ttttgaggct cttgacataa tatcctgata tgttttcgga tgttctttaa acaattcgtc
     4981 cgccttcata ggtttgccgt taacattaat ggtgatcttg tcaacaggaa atcccgcttc
     5041 aatacaggcg gcaaaagctt gcctgcccct ttccatcgct accttttcac tatttggaaa
     5101 attacaattc atttcaatag agtcataacc tgaagctttt atagcttgag caatgagcat
     5161 catgtctgtt ttgacatttt gccttggatc ctgatagtag aatgggttta tcatcggcat
     5221 ctggattgca aaactgccat ccggttgttg atgaatctct ctccctgtaa tacttttaat
     5281 gacaggaata tctttgagtt tgattccatt gagatcaatt ttgttttctg atatacctac
     5341 ttgaactgtt gtagattcag gaggaagccc ttttctttgg cgttctttgg catataactt
     5401 ttcaagcgtt tgacgcattt ccttattgtt ttcatacaga ttggcaagaa acagatactc
     5461 tgatgattca ctcgcagagg ctttatgtag ttggtttaac gagtcgcttg tggatttatc
     5521 gaattcaccc agttgttttt tatgggtttc ttccaattcc ttaatcaagc cttctgtaac
     5581 tttcttaact tcgtcattat ccttaagacc cagtgctttt ttaaaatcgg atacgtaatt
     5641 accagcttgt ccttctttct ggggatccaa tttttctttc aacatttgga tttcatgcgc
     5701 ttgctgttct attatggctt tacgagcatc atcagtcagt tccttcatgg tgccaacaag
     5761 atcctgggct cggaatttgc ttggaattaa tttggttagc tctttaaatg ttgaagttga
     5821 ttctgtaaaa tttttttgtg ctgttttaaa tttttcagtt ggtgccagga ctgttttact
     5881 taagacatcc cggatattat ccccttcggt agtattggct tccttcagtt tatcatgaaa
     5941 cagcaatccc ccgggtaagt tggttttcgg aaaagcccgg aaatcatcta ctctggagct
     6001 gacaagctta tcgaatgcgt cagaactaaa gcttgagttt tctggcaaac cgatatgatc
     6061 tctgtattct ctctctagcc ttttaaattc ttcagaagtg tatttttttg cacggctctc
     6121 tgattttaaa tgtttaccaa ttaaagggat agaattataa aaagccatat caaatacctc
     6181 aataaggtta tctagataaa gtataacagt tttttattaa gtaaaaatta agctaattta
     6241 ttaataatta ccaggctggt attgcatctt ctccaaacag tttttttgct ttctctttta
     6301 cttcttctga attcaacgcc ttcacaaaca attcaagctg tggccttttt tcagtgtcac
     6361 ggcgtatcac aatgatattg gcataaggtg aatctttgcc ttcagtaaat aaagcatctt
     6421 ttgatgggct taatccggca ggcaaggcaa acgtcgtgtt aattactgcg gcatcaacat
     6481 cagggagcac tcttggtaat tgagcggcgt ctatctcttt gaatttgaat tgtttggggt
     6541 tactttcgat atcttgaata ccgctattcg ttgtattttt aagagtaatt aaatgagctt
     6601 tctccaataa caagaaggcg cgcatctcat tactagggtc attagggact gcaattattc
     6661 cattctcagg taattcactg agtgttttat atttcttcga ataaataccc attggataaa
     6721 cgaaggttct gccgattgcc tgcaaatcat aaccatggga cagtatagca gcttttaaat
     6781 aaggtaaatg ttggtaaaca ttagcatcta gactgccatc ttgcagtgct tcattgggca
     6841 aattataatc attaaattca acaattttta tgttgagccc atactctttt tcagcaactt
     6901 gttttgctgt ttcaataagt tctgtttcag ggccagcaat tgtgccgatg accaaggtat
     6961 tgggagctgg cttactactg caagctacca ggctaatgat taaaaccaaa caacttaaaa
     7021 ttctcattga acctcccaat taataatgtg tacaacgttt ggccaagtag tctccaacca
     7081 tttgtatcag ttggaccaaa ataattaata caatcacagt agctatcata atgccagcat
     7141 taaagcgttg ataaccatag ttaatcgcta atgtacctaa tcctccggca cccacagctc
     7201 ctgccatggc tgaataatta atcagagtga ttgctgttac tgtaatggaa tgaattaatc
     7261 ctgattgtgc ctcaggtaat atgatatgta aaataatttg gcgtgtgtta gctcccattg
     7321 aataaccggt ttctataaga ccaaggggta acgattggag aacattatca actagccttg
     7381 caaaaaaagg agtcgcacca actgttaatg gaacgattgc tgcattgatc cctatgctgg
     7441 taccaacaat tagcctggtg aatggtataa ttgcaacaag taaaatgata aaagggatgg
     7501 aacggaaaat attaatcaga gcagataaaa ttttattcaa tttaatgttt ggttttatcc
     7561 gagatgatga gtacagcaat atacctaaag gcaagccaag gagtactgca aatagagtac
     7621 taaaaaacac catatacagt gtttctcctg ttgcgttaag taaatcataa accatctggt
     7681 aggacatagc ctaaaacctc cacagtaata ccagcttgtt cgcaacgatt gataaatgct
     7741 tcaagcaata atggattcgc tgttaattca acaaccaaaa caccacaagt cactccatcg
     7801 aaacgatcaa tatttgccag tagaatattg atgtccatgt ttaattctct gctggtttgg
     7861 ctaataaaag gtaccgttgc ttcctcgccc tgaaaaaata atctcacgag aggtttttca
     7921 gtagcatagt ccgccagcct gcgagtaagg caggttggga gttcagggct aatttgtgcg
     7981 taaagcattt tgcgagcgag actctctggc ttattgaaaa tattagataa agcagtagtt
     8041 tcaacaatct tgccatcgac catgacagac agtctttggc atatacgttt tacgacatcc
     8101 atttcgtgag taatcaagac aatagtaatt ccatacaatt cattgatttt ttttagtagt
     8161 gataatatgg cgtcagtagt tgcaggatcg agggctgaag tggcttcatc acataataga
     8221 actttgggag aacaacttag tgctcgtgca attgctactc tttgtttttg cccaccacta
     8281 agttgtgagg ggaaagcatc tttcttatct gttaactcga caacaggaag caactcttca
     8341 attttctgtt tgatcgactc ttcgtctata ccttgaattc tcatcggaag ggctatgtta
     8401 tcaaaaacag tttttgaatt tagcaaatta aaatgttgga agatcatggc aattttatga
     8461 cgtgccaagg ctaaatcttt tctggaaagg ctcatcagat tctgcccatc aataacgact
     8521 tcaccttcgt caggtttttc caatatattg atgcaacgca gtaatgttga ttttcctgcc
     8581 ccgcttttcc ctataactcc aaaaatttca ccttcctgaa taaataaatt aatatcgttt
     8641 aaggcgagtt taccagagaa agattttttt agcccacata attcaatcat tactataacc
     8701 tgggaatatc gcttgcgggg cataaagtgg ggattagaaa gcaaccgctt tagccgtatt
     8761 ttcaaaagat atctgcagca ctggaaaaca ataattctta ataaaaagca aaaattgcat
     8821 tttacctcga ttttagagtt ttcatgttct taaacagagg aataaatctt ttgagcgtcc
     8881 gcaagctgtg tttcaaatcg acgctatgca caagtataca caaactggat tcataaacaa
     8941 agaaaaatct taattctgtt gagtatctcg atttgttttt catgattatt ggttggcttt
     9001 tggtttatat aaaaagagat aattcgcatt aaccagattc gcggtgtcat tcggtctgaa
     9061 aataattatt ttggttgagg attgacaaga taaattctta ttggcaacct taagccggtt
     9121 ttaatacaag gaacattatt catgaaaaag atgtttttag cagtttttac cttgtgctca
     9181 ttcacagcgc aggcagcagt agaattaaac acttttcaaa atatagtaga cgctatagct
     9241 gaaggaaagc gaatcacttt tgtaattaac ttaaaaaaat gtacttcgga aatgccattg
     9301 aactctgcaa tagtttcagt tactccgaat gcagttatgg taatcggtga tagccgtgtt
     9361 acagcctcag ataggcattt tactcttgat gatccgctgg ctcgcggtac acctatgttt
     9421 gattacagta agtttaattt agattctgag ggtgatgctt caattaaaac cactgtgctg
     9481 aatgcaagta gttatgagcg attaggaagt tatcaaatga attgtaaatt aggggatggt
     9541 tttaaagtat tcgggtaaat gagttttcct caaattatga ggattattaa attttttcat
     9601 tcaaataata ttcaaataac aagtatttac atgaaagggc aactcttgca gttgcccttt
     9661 ctattggttg atatgagatg aattttaatg tggattcgtg ctgaaaatag ctattttcac
     9721 gcaattttag cttttcaatt cgttcctgtc tttaaaatat tctaatgctt gagggtttgc
     9781 caaagactgt aagttactta ctgattgacc atgaacaact tgcctgacgg cgagctccac
     9841 tactttcccg ctaagagttt taggaatgtc tgggacttgt agtattttag ctggcacatg
     9901 tcgtggtgag gcattttttc taatggttgt tttaatcaga ttgattaatt catcatccag
     9961 ttttttcccc tcacgcaact taacgaataa aacaactcgt acatcatcct gccaatcttg
    10021 cccaatgact atactatcaa caacatcatc tactttttca acttgtctgt aaatttcagc
    10081 tgttcctata cgaactcctc ccggatttaa tatagcgtcg gaacgtccat ggataattaa
    10141 tccattgtgg gcagttattt cagcgaaatc gccatgtgcc cacacccctg ggaagcgctc
    10201 aaaataagca tgtttatatg cttgtctatc cttatcattc caaaaacaga ctggcatgga
    10261 agggaagggt ttggtgcaaa ccaactcacc acgtttttgt ttgattgaat ggccttgctc
    10321 atcatacacc gcaacttcca tacctaatcc aatgcattgt aattcacccc tataaaccgg
    10381 gagaatagga tttcccaatg caaaacaaga aataatatcc gtccctcctg aaatagagct
    10441 taattgcaga tcttttttaa tatgttcata gacgaaatca tagtttttag gtagaagagg
    10501 ggagccggta gacaagatac agcgcaactc ggataattga aattcacttt ttggttttac
    10561 cccggctttt tcaatcgcgg atatgaactt ggcgctggtt ccaaatacag tgactttttc
    10621 ttcatctatc agtcgaaaca ttctattatc tgtagggtag gttggagaac cttcatatag
    10681 agtgatggta gctcccaatg ccaaagcaga aacagtccag ttccacatca tccagccaca
    10741 cgttgtataa aaacataaat tgtctgcgga tttaatatct gaatgaagac ctaattcttt
    10801 cagatgctga attaaagtac ctcccgcgcc atgaacgata catttaggtt tacctgttgt
    10861 tcctgaagaa aacataatat aaacaggatg atcaaaaggc agagaaataa aatcacattc
    10921 ttttaccggt tttataaaat cattccaata cgatgtgtta ggcagttgtt cgagattaat
    10981 ttcctcatta atatttgggc aaattactac ctgtcttaga gaagtaatcg cagcagtaag
    11041 ttgtacgacc ttttcagagc tgctgtgttt cttaccttga tattgatgac catcacaaat
    11101 aaagagtact ttaggttcaa tttgtcctat cctgtcaagt gcagcctggg taccaaagtc
    11161 aggtgagcag gatgaccaga ttgcccctaa agaagttgtt gccagcatgg caatgattgt
    11221 gtaagaagta ttaggcatca ctgcggccac ccggtcgcca gtggcgacac caattgcttt
    11281 aagaccagca gcgcattggg ctactttttc atataattca gcatagctaa tgacactttt
    11341 gcagtcattt tcatcaaggc ttattagggc agggtgatta tccctgcggg ttaataattt
    11401 ttgagcgaaa ttgaagcgtg ctcctgaaaa ccagcgtgct tcaatcattt cagaataata
    11461 attcagaatt tggtgaggtg gtgtatcaaa gttcaattta aagaaaaggc aaagagtttc
    11521 ccagaatgat tcaggatgct ttactgacca ggtatgtaag tcttgataat tttcaaaaac
    11581 ttgacaatgc ctttttgcgg caaagtccat aaattgccac attcttgttt gttcagggtt
    11641 tttaggtgtc catactttgt cgttcatgaa aatttctctc atagttctgt tttgaatgat
    11701 gggtgttaca ctggaaaatt tcatcagtaa tttgtttata agtataggct agcacaaaac
    11761 tgggtgcttc tctttaagtt tccgattttt agtacttatc aaagccaata atgatggagc
    11821 ttcttaatta catggattgg ctagcatagc attagctact ttggattgat tttttcgccc
    11881 tagtgctttg caaatcatat ctccggcagc aacaatttta aatatatcta tacctgtatc
    11941 aatacccaat ccatgcatca aataaagtac atcttctgtt gcaacattgc cactggcacc
    12001 tcgagcatat ggacaacctc caagacccgc aacagagctg tcaaagcggt taactccata
    12061 ctcaagggag gcataaatat ttgctacggc ttgtccataa gtatcgtgga aatgcatggc
    12121 taattgtgtg attggaagta tgggtagtat ggcatcaaga agcaactgag tttgcctcgg
    12181 agttcccacg ccaatagtat ctcccaacga aatttcgtgt acgcctagat caagaagcat
    12241 tttagtgaca ctaacaacct gactaggttg tatatatcct tcatagggac agcctaatac
    12301 gcaggagata tagcctctca ctctaatttg attggttttt gctaatgcta aaacaggttt
    12361 aaagcgttct atactttctt taatagaaca attaatattg cgttgattga ataattcact
    12421 tgcagcagtg aatactgcaa tattttgtac tcccatttcc agggctttaa gcattcctcg
    12481 ttcgtttgga accagggcag agtagttgat ggagggagtc ttattaatag attgaaatac
    12541 ttcttcatta tcagctagct gtggaattgc ttttgctgaa acaaaactgg tgacttctat
    12601 ctctttcagc cctgtctgac tgagtaaatt gattaattca atttttttat cactatggag
    12661 aaaagatggc tcattttgta agccatctct aggacctact tcgattattg tcacatgctg
    12721 aggatagtcc ataaatattc tcttaagtat ccgattcgct aagtgctaac agttctgctc
    12781 cctcactcac ttgggaacct acagaataaa aaatatctga taatatacca tcgattggag
    12841 catgaatggt gtgttccatt ttcattgcct caagtacaat taagctttca ccagctttta
    12901 cctgttcacc aatatttttt aaaatagcaa cgacagtggc aggcattggc gcggttaact
    12961 ggcctttatg ggcggaagtt tgagcatcca gtttactcca gtagaagcgt tcgattgaaa
    13021 gttggccttt gtcagtatag aagatcaaat ggtgatcttt gttttctact ctggctttca
    13081 ctgatttttg atcaatttca ataataagct ggtcaatgtc atatctggca tagatgacca
    13141 tttccttgtt ttcaattttt gctttaaatt ttttattatc tataggtgta atctgagctt
    13201 caattatcgt ggagttcaat tgataacgcc atatccaatg gctggaaaga tgcatttgcc
    13261 agccaaatgc ctcttgcaac aagggatccg tagtgcgatt aatcatacct aaataatcat
    13321 aacttatggc caggagcata cctaactcat tatctggttt aggcaggctt atgttttctt
    13381 tctctaaaaa atcagtactt aattttgcct ctttaaattt tacatgttgg caaatggctc
    13441 gtagaaaggg tatatttgtt tttaccccgc caatgtcata gtgagccagg ctacgctcca
    13501 atctatgtaa tgcttcttca cgattatgtc cccaggcaat cagcttggct atcatgggat
    13561 cataatacct tgttatttct gaagacaaag taacacctgt atcaatgcgt atgccatcgc
    13621 cagagggttc ttttaagaat tgaagttggc caatagaggg tataaaaccc tgataaggat
    13681 cttcagcgta aatacggcac tcaattgcat ggccttgagc ttgaatttga ttttgaagca
    13741 aaggtaatgt gtcattcgct gcaattttta tctgccaagc gacaagatca agtcctgtaa
    13801 tcatttctgt gacaggatgt tctacttgaa gtcttgtatt catttccata aagtagaatt
    13861 tatcttcacc gtctaccaaa aactctactg ttccagctcc tctgtagttg atggagcggg
    13921 ctacttcgca ggctgcttct gccagccttt gcctaagcac gggcaataaa ttgggagcag
    13981 gggcctcctc gataattttt tgatgcctgc gctgaatgga acaatctctc tcaaacaaat
    14041 ttacaacatt tccatgatta tccgccatga tctgtacttc aacatgacga ggattaagaa
    14101 ctaatcgttc aattatcata gtatcatccg caaaggaggc catagattct cttttggctc
    14161 ctgctaatgc atcatgaaac tctttttcat catgtactgc tcgcattcct tttccaccac
    14221 caccattagc ggctttaatc agtactggaa atccaatttt tttagcttcg ctcaacaact
    14281 tttcttcaga ctgttcaacg ccatgatagc ccggtgtaag tgggactttc gttttttcaa
    14341 gaagttgttt agctaattgt ttggatgcca ttgcttccat tgctttaatg gagggaccga
    14401 tgaaaacaat gcccgcttgt tcacaggctt tggcaaaatc aggattttca gataaaaatc
    14461 catagccagg atggatggct tgtgctccac tttctttagc tgcctgaata atatggtcaa
    14521 tattcagata actttcttta gctggcgctt ctccaatata ataagcgcta tcagctaatc
    14581 gcacatgaag gctgtttcta tctgctgctg aataaacggc aatagcctga atacccatag
    14641 aatgtgctgt tttaatgatt cgacaggcta tttcgcctcg gttagcaatt aatattttat
    14701 taaacatgtc cagaaccttt attccagttg ggtatttctt tattgagaaa tgctttaagc
    14761 ccttcttgcc cctcatcaga tactctttta tgagcaatca gagaagcagt gtagcgaacc
    14821 agttcttcat ctattttttt attagcgacg tattgtgcca gttgttttga gtttttaacc
    14881 gcttctggag cattattact gatttgcgag gcgtatttga gagtaaactc caacaacgta
    14941 tcatcaggga cgcagtgctg aactaaatta agagaatatg cacgtgtggc atcaaaaact
    15001 tcagcactca tgaataacat ttttgccgct cgttcaccta ttgctctaac tacataggga
    15061 ctaattactg ctggaataag acctaatttc acttcagaaa aacaaaaacg tgctgaagta
    15121 gatgcaatgg ctatatcaca tgctgctgcc aatccagctc caccaccaaa agcggcacct
    15181 tgaaccatgg cgatagtagg ttttgggctt tgactgatgc tatacattaa attacctaat
    15241 accagactgt cttccaggtt ttcttcttca gtaaagttag ccatgctttg catccaggtc
    15301 aaatcagcac cagctgaaaa atgctttcca ttcgctttaa gtactatgac gcgaacgtta
    15361 gtatcattta ttgcggagtc aagtcgtatt ctcatttcag tcagcagttg attatcgaac
    15421 gcattatgct tgctaattcg attcatagta agtaaaccta ctttatcttg aatttcatat
    15481 aataaatcac tcatgcctat tgttcctttc cttttacatg cgaaatacac cgaaacgagt
    15541 atcctcaatc ggtgcgttta aggctgcaga taatccaagt ccgagtattt tgcgtgtatc
    15601 ctgaggcgcg atgaccccat catcccaaag cctggcactg gcatagtaag gatttccctg
    15661 agtttcatac tgacttctca tttgagtttt aaattgttct tcttcttcga gtgaccattc
    15721 ttttccttgc tttgcatatt tctctctggt gatttgcgcg agaacattcg ctgcttgttc
    15781 accacccata actgagatgc gtgcattagg ccaggcccat aggaaacgag gagcatatgc
    15841 ccggccacac atggcataat tacctgctcc aaaacttcca ccaacaatga tggtaaattt
    15901 gggtacatta gcattggcaa ctgctgtcac cattttggct ccatgttttg caatacctga
    15961 tgcttcgtat ttggagccca ccataaatcc tgtaatattt tgcaggaata caagtggtat
    16021 tttacgttga cagcacagct ctatgaaatg gctgcccttt tgcgcacttt cactaaaaag
    16081 tatgccatta ttagcaataa tccctatagg atatccatat aggcgagcaa aaccgcaaac
    16141 cagggttgtt ccaaaaagtg ctttgaattc atcaaactct gaaccatcta ccactcgagc
    16201 aataatttct cttatatcaa aaggctttct agggtcagtg ggaattattc cggtaagatc
    16261 ttcgctatca taaagaggag gaactgtatc cactctatga atggaatctg gttttttacg
    16321 atttaaattg ctaatggcga ctcgcgctaa atgaagcgcg tgagcgtcat tttccgcata
    16381 atgatcagat actcctgagt ggcgacagtg aacttcggct cctcctaatt cttccgcact
    16441 gattacttct cctgttgctg ctttaacgag aggagggcct ccaaggaata tggttgcctg
    16501 attcttgacc ataatagatt catcagccat ggctggtaca taggctcctc ccgccgtaca
    16561 agatcccata accacggcta tttgaggaat atttaatgct gacatttgcg cttgattata
    16621 aaaaacacgt ccaaaatgtt ctttatcggc aaagacctga tcttgaagcg gtagaaaggc
    16681 acctcctgaa tccaccagat agatgcatgg aagatggttt atcaatgcaa tttcttgtgc
    16741 ccttaagtgt ttttttacgg tcagaggata atatgttcca cctttaacag tggcatcgtt
    16801 gaccacaatc acacattcgt ttcctgccac tcttccaatg ccagtgatta ttcctgcagc
    16861 gggtattgtg tcttcataga cttggtaggc agcaagttga gatagctcaa gaaaaggact
    16921 acctggatcc agcaactggt gtaagcgttc tctcggaagt agtttgcctt gttgttgatg
    16981 tttggtacgt gctttttcat ctccacccag agagatttga tgtatttttt ctcttaaatc
    17041 agtaaccaat gcttgcatat tcgcctgatt atttttgaat tcctgactgc tggtattgat
    17101 ttgagtagtt aacttagcca ttttttatcc ttaccgaatc aaaatatcac tcaagactat
    17161 gatggtccat attatccaga caatcgcaat aaaatggggc atgtcttttc ctttgagtgc
    17221 ccggtaaatt agcgctggga ttcctgcgat gattatgggt aataacataa ggactagaat
    17281 tttcctgata atcagtcccc aacctgtttg gctgaatata ggtgttaatt ttaaattaac
    17341 gtaagtgaaa aacatatcga tgtaaactac gataagatgg gcatattttg caaatacgac
    17401 aataagaata cttagtagaa ggtaaatgag actatgtctt agcatgtaca tcctttatta
    17461 ttgttataac caagttggtt ttgataggta aaacattatc tggtcgcaaa taaaaatagc
    17521 aaccagtttt aatataaaaa cctgggtaaa aataaaggtt ttacccaggt catctttaac
    17581 cgttaaatta gctcaattgc catagctgtt gcttcaccac cgcctataca taaagaagct
    17641 attccgcgtt ttttaccctg gtgttttagc gcatgcatta aagtaaccag aatgcgagct
    17701 cctgaagcac caatgggatg ccctaaagcg catgccccgc catgtatatt gacttgttcc
    17761 ggattcaatt caagctgagt aattgctgcc atcgctacta ctgcaaacgc ttcattaatc
    17821 tcaaaaagat ccacatcatc ttgtttccat gacgctttgt tcattacctt acggattgca
    17881 tcaacaggag cggttgtgaa ccattgtggg gcttgggaat ggctggcgtg tgctatgatt
    17941 tttgccaagg gcttaatgcc acgtttttct gcgtttgagg cactcattaa aattaaactg
    18001 gccgcgccat ctgaaataga acttgaattg gctgctgtga ctgtaccatc tgcttgaaag
    18061 gctggtttta actgaggtat tttggcaagt ttggctgtct caggcccttc atcgacgtca
    18121 accgtgatat ctcctttgcg ggtagttaag gtgacaggag cgatttcttc tcgaaaggca
    18181 ccattctcta ttgcttttag cgctttggtc attgagcgta aggcatattc gtcttgttgt
    18241 tctctgctga aattaaagtg ttttgcagtt gtttcagcga aacaccccat taattgtcct
    18301 ttatcatagg catcttctaa tccatcaagg aacatatgat ctttcagttc accatgaccc
    18361 agtctatatc ctgctcgtgc tttggataat aaataggggg cattgctcat actttccata
    18421 ccgctagcca aaattacatt agctgtacct gccttaatta aatcatgcgc aagcatcaca
    18481 gccttcatgc ccgaaccaca cattttatta atggtcgtgg ctcctgtcga attaggtatg
    18541 cctgctttta ttgctgcctg tctggcagga gcttgaccaa taccagcttg taatacacaa
    18601 ccactgatca cttcatcgat ttctgccgga gcaatacctg attgagacaa cgctgccata
    18661 tgcgctactg cccctaactc tggggaggaa agtgcagata aattgcctag catagctccc
    18721 attggtgttc tttttgctgc gactatcacg acatcattat gttccattgt ctcattcctt
    18781 atgctgtttc tttaaaaagt tcacgaccaa ttagcattct tcttatttca gaagtgcctg
    18841 ccccaatttc atacaacttc gcatccctta ataaacgtcc tgtaggatat tcattaatat
    18901 aaccatttcc tccaagaatc tgtattgact gtaaagccat ttgtgttgct ttttctgcag
    18961 tatataaaat aactcctgca gcatccttac gactaaccct accgttgtca caagccttgg
    19021 caacggcata cagataagct ctgcaagcat tcaaatcagt gtacatatca gcaagtttgc
    19081 cttgaataaa ttgaaactca cctatcgcct gattaaattg cttgcgttca tggacatagg
    19141 gtaatacaac atccatacaa gcttgcataa ttccaactgg tccggcagca aggattgtgc
    19201 gttcataatc caagccgctc attaatacct tgaccccttg atttactgtt cccagaacat
    19261 tttcagaagg cacttcacat tgatcaaata ccaattcaca tgtgtttgac ccacgcatac
    19321 caagcttgtc tagtttttgt gctgttttaa atccgggcat tcctttttcg ataataaagg
    19381 cagtaatccc tttgcttcca gcctgcttgt ctgttttagc atagacaacc aaaacatccg
    19441 cgtcaggacc attggttatc cacatttttg tgccgtcaag aatatatttg tctccagcag
    19501 aacgggcttg taattgcata ctgactacat cggaccctga gttggactca ctcatcgcca
    19561 aagcaccaat atattcaccg cttataagtt taggcaaata tttttgcttt tgctcattat
    19621 ttccgtttaa aaagatttga ttgacacaca aattagagtg cgcgccataa cttaaaccaa
    19681 ctgatgcgga ggctctggaa atttcttcca ttgctatggc atgcgcgagg tatcccatat
    19741 tggcaccacc atattcttca cttactgtga tgccaagtaa tcccatttct cccagttttc
    19801 tccagagatg gttaggaaaa gtatttttct catcgatttg tgcagctaaa ggagctattt
    19861 cagtttgggc aaattggttg acgctgtctc gtaacatgtc atacgtttcg cccagttgaa
    19921 agtctaagcc tgtgtacatg agtgacatcc tgttatgttg ataaatacgc tagactatac
    19981 ccaaatgaag cgtacttgtc accttcctgt ttatttccaa gacaatcttt ttaacccact
    20041 gatacatttg ataatatacg gatgtcttat tgcgaaaaat tgaaagaatt tgaatggtaa
    20101 atattcattt gttcaatcgc aaggtggctt ttctctccaa actttttaaa tttttatctc
    20161 aaaaacacct ttacccaggg cagtggttaa aatgattctt tacctgggta aaaaaatcac
    20221 ctaatcttta gccaaagtgt aatccaaatg gatttagaag aggagtattt ctaatgagaa
    20281 aactggtatt atgggggcat ggaattgaag actatcgtga gatgtttgat ttaccccagg
    20341 gagtggagaa ttcacgacta ttagaatacg gttgcggtcc tagcgctgtt aattaccagc
    20401 aaactcaagt gaaaaaaaat ggagtagtga gttgtgaccc tttattcgta ttggataaag
    20461 atacgctgct tgctaaagca aaaatgattt ttgcgaatat ggctgaagag gttaggcagc
    20521 atcaggatca atttgacttt agccgtaatg gtagtttgga taaattgctt caggaaagac
    20581 aagaaggaat gaagcagttt tttgcggatt atgaacaagg taaggctgat ggaaggtacc
    20641 tggggcttac cgattatcat ttaccatttg ccgattttac ctttgatttt gctctgagtt
    20701 ctcattattt ttttgcagat cttgacgatc aaacggttga ttttcacctg attgcaattc
    20761 gtgaattagc aagggtagcc aaagaagtga gaatttttcc attgattgat agagaaggta
    20821 acacttcaca atttctagga ccggttttac taggcttgca acaggagaac tatggagtgg
    20881 aggttaggga ggctgctttt catttgcata aatcaggtaa tgcaatgcta agagtttggg
    20941 cgcaacaatg tgttgtttaa ttgagattac ttttttaaag taagtgactg gtatgcaggg
    21001 aggaaaaaat ggcagtatca aataagaaaa acagtgagtt tacaccttcc ttaagatctc
    21061 acatgattgc tgcgttatta agtatgggag cagctgcaat tatcttatgg ttggcgtcgg
    21121 ggatgacatt agctgctgtt atcgttgact ccttcagttt gggtttttat atagggttta
    21181 tgctccttat gagcgccgct ccggctgtgt tggcttcacc agttatttta cctggtttac
    21241 tacttgttgt atcaggattg tttcttggag cggttgctgc aacgtttgcg catcggttaa
    21301 aaaataatcc cagtttgcca caaaacaata atggaaatgg tctgatcaat gaattaacaa
    21361 aagataaatc tgagttgctg attagtcatg atgatttaca gtgtcaatcc aactctttgg
    21421 aaggtgaaaa cactttatct caacagaatg aggataatgt taccggggaa aaatttggtt
    21481 tcatgaaatt ttggggaagc ttgggtttga cttcgtttgc tagttcagga gcgactgctt
    21541 tattgggttg gcttgctaca gattttgtag tttctctctc tataattcct gtgtttttgc
    21601 taggaatgac tctagccatc gttttagttc cattgtcaat tggattatcc ggaatttttg
    21661 cgttaccagc ggttactgcg cttatgtttg ccttgggagg ctttgctaca ggagtgtttg
    21721 ctgctaccct ggctgcatat tttatagctg ataatcaggc gcaagatcat cctgttgagc
    21781 caataatcag aagctcgatg gagaggcaat gcaaagatca gggttctcat caaaaaattc
    21841 taaatggatt aaaaaaagat aaattgagtg ctaatgaaga agtgattaat tctgatttaa
    21901 acgaacaaaa atcaagaggt ccaaaaacat caaagagtga tactggtgtt aaggaagaac
    21961 atattaatac tgcagaggaa tacagttttt gtggtttaag attctagctt tattatgagt
    22021 ggaccaacga ttaatagaga cgcgcctatg attttccctt ttagacaggg agaatagcct
    22081 atcagcagaa taagctcgtt tatgcaacag gactatttgc aatcataatg agcaagtcat
    22141 ttcgctattt atctgggcag atgcataaaa aattatctga attaatttta tttcgcttcc
    22201 tattaatcgt cagtacgaaa atcatcacga aaaatgttat catacctcaa ttgttcttta
    22261 ttaatgattc tccctgcttt aggagtaatt ttatgtattc actgctgcgc cctttacttt
    22321 tcagattgga tgcagaaaaa gcccattcat taacattatc cttattacat tacctccctg
    22381 gtttttattt tcggaaaata gccggtcaac ctgttcatgc tatgggtctt gtattccctc
    22441 atcaagttgg actggcagct ggtttagata aaaatggcga acatttggat gcattagcca
    22501 aattgggatt ttcattcatt gaattaggga cagtaacacc taagggacaa gcaggtaatc
    22561 ccaaaccaag gctttttcgt attgcagagg caaatgcgat tatcaacagg atgggtttta
    22621 acaattcggg agttgatgta ctggtagaaa atgtaaaaag agcaaattat aaaggaatat
    22681 tagggataaa tattggaaaa aataaagaga ctaatttaaa tcaagctgca gaggactatc
    22741 tttattgctt ccgtaaagta tatgatcatg cttcctatgt cactatcaat atttcctctc
    22801 ccaatacccc tgatttacgc cagttacaac aaggagatta ttttgctgaa ttactcaccc
    22861 agttacaaaa agaacagata aaattagcag atcaatatgg acgccatgtg cctttagtgg
    22921 tgaaagtatc tccggatgaa acagatgaaa cactaaaaca aatgacagat atcattttac
    22981 agtatggaat cgagggtatt attgcaacga ataccacctg ttctcgggaa atggtaaaaa
    23041 atttgccttg ttctgaagaa cagggcggat taagtggtag gccactgatg gaattatcta
    23101 ctcgctgctt gcgcttgttg aaacaatatg ttggtaatga tgtgacattg ataggagtgg
    23161 gtggtattga cagtttggag agcgccaaag acaagatcaa tgcaggagct tcgttattac
    23221 aagtatacag tggtttagtt tataagggac cggagttgat tcacgatatt gtttcaggtt
    23281 taaatgctat ctgatcctgg tggtgaggaa atcgatgtat attactaagg aattggctgt
    23341 tcgcatggaa gggtgtatca agcagagtca cattgaggtg acaaagcggt actcacaagg
    23401 aaaaattctg gaaatcaacg gcggagcagc ttgtttttct ggttttgagt catttctatc
    23461 ccaggtagta ggctggggat ttaaaacaga gcctaaccaa tataaagctg aaatagaagc
    23521 tatagaaaat ttttataaat cgttaggcca taatcgtgtc gatattgagt tatgtccttt
    23581 tgtgggaaat catttaatta attttttaag ccaaagaggt tactacataa ctgagttaaa
    23641 taatgtttca gttcttgatt taaagagtta ccatctggtt gctcactccg aggatgatgg
    23701 gtttattata agagaagtga agcagtctga attggaagag tgggcaaaaa gagtagctat
    23761 tggatttggt tttcttgagg ctcaggaaca attttaccaa tatgccagtg caaaaggagt
    23821 tttagcattt gcagcatatg atcatggtaa tttggttgct ggcgccacaa tagcaattca
    23881 tggtgatgct tgtgatttgg gcgttaccag cacattacct ctttatcgag ggaaaggcct
    23941 tcagaaaaaa ttattgtttg ctcgtctgaa tctggccaag cagtatggtt tggaattcgc
    24001 caccgtaact actcaaccag gtacagtttc agatttgaac gttcaaaagg ctggatttcg
    24061 ttgtgcctac accagaataa aactaactat ggaataggtg gacttcatga tagaacattg
    24121 gcgcttagac agaataagag ttacaattca taacatttgc gtacaagatt aacttctaaa
    24181 ttaatgaaaa ataacctccc tattaaatcc cgtctgctat acaaacgctt gttaagctat
    24241 gtaaaacctt tttggccagt tttattatta ggagtattgg cgaatatcct atattcgggc
    24301 attgacgccg gctttaccta tatgaccaag ctttttttgg ataaaagctt tattactatt
    24361 gatctgaatt ttgttaaaca aatcccgctg attgttttaa tcggtattac tctgagagga
    24421 ttggtcagtt ctttaggcag ttattgtatg acatgggtgg ctcgctcagt agttaaagta
    24481 ttaaggcaga ccgtattttc tcatattatt cacttaccgg cggattatta tgacgaagcc
    24541 acatcaggtc agcttctttc aaaaattttg tacgatgttg agcaagtggc acaagtcagt
    24601 gcggatgccc taacagattt tattcaaaat atatgtctgg tcatcggttt gctgaccgtt
    24661 atgatggtta tatgttggca gctgtctttg atgtttttat taactatccc ctttgtggga
    24721 atcattgtta attatactaa taaaagagta agacgaatta gtcacaaagt ccaaaaaacc
    24781 atgggcgaag tcacagaaat tgccagtgag gcaatcgagg gttatcgagt ggttcgcatc
    24841 tttggtggtg aacggtatga aataacaaaa tttaataaag caacagaata ttcacgcaaa
    24901 aacgatatga aagttgccat aagcaaggcg attaatgttt caggcgttca attggtgatc
    24961 gctatcggga ttgccacgat tattatggct gccattcatt tatctactgt cattactatc
    25021 agtgccggtt cttttttggc aattatcgca gccatgttgc aattaataaa acctatgaaa
    25081 actctgacta ctcttaacgc aaccatacaa agaggcttgg ctggtgcaga aagtgtattt
    25141 aatttgcttg atttgccttt ggaaagaaac aatggtttga tattaaaaga aaagatacgt
    25201 ggtgaaattg agtttaagca tgtataccac gcataccgac agggtcaaaa tattttacat
    25261 gatgtcaatt ttgtaattga agcaggaacg tctgttgctc tggttgggca ttcaggaagt
    25321 ggtaaaacga ccatagccag tttattaccc cgcttttatg aattgagtca aggtatgatt
    25381 actttagatg gcatgcccat tcaacagctt agtctggaaa gtttacgcaa gcagatgtcc
    25441 ttggtcagtc aaaacgtgac actttttaat gatactttgg ctaacaacat agcctatggt
    25501 cgttttgatg cgagccgaga gcaaattatt actgcggcta aactggccta tgctgatgag
    25561 tttattaagc aattacctga tggctatgat accagggtcg gagaaaatgg ggtgttgttg
    25621 tcaggcggac aaaggcaaag aatagcgata gccagggcaa ttttgaaaga tgctccaatt
    25681 ttaatactcg atgaggcgac ttcagctctg gatagtgagt ctgagcatta tattcaggcg
    25741 gctttggagc aagtgatgaa aggtagaact actttaatca ttgcgcaccg attgtcgact
    25801 attaagcatg ctcacaagat tatcgtattg caacatggtc gtattgttga gcaaggtagt
    25861 catcaagaat tacttgatat ggatggtcat tacgctcagt tgtataaagt gcaacaattt
    25921 ggcaggatta atgaggaagt ggtagcttaa tgtcattttt tgtgaacagg atatggtatg
    25981 gtaaccattt tttacagtgg atactggttc cattttcctg gttatacaga attgttatac
    26041 gaaccagacg atggtattta cagcgtttct gccaacagct gtaccctata ccaattattg
    26101 tagtgggaaa cgtgaccgta ggaggagtag gcaagacgcc tctggtcatt gaaattgcta
    26161 aaaaaataca gcaaaaaggc ttaaaagtag ggattgtaag ccgtggttac aaagcagcaa
    26221 taaaacattt tccatacgaa gtaaaattaa atgattcagc tgaattagta ggcgatgagc
    26281 cgctcatgat ggcaagaaaa attaattgcc cagtggttat tgcgcctaag cgcaatgaag
    26341 cagtaaggta ccttttagac aagcactcgg ttgaaattat tatcagtgat gatggcttgc
    26401 aacattataa aatggggcga tctattgaaa ttgtagtaat agatgggatg cgaaaactgg
    26461 gtaatggttt ttgtctaccc gctggaccat taagggaacc tgattcacga ttaaaacaag
    26521 ttgattttgt tattgttaat caaggtgctg cagaaggtac ttattctatg gaattaattc
    26581 ctaaaaacat agttcgattg tcaactcagg aagaagtcag taatgatttg tttacttctg
    26641 aagtagcagc tgtagcagga attggtaacc cgcaacgatt ctattctact ttaagtcaat
    26701 taggtataaa atttaatcca tattcttatc ccgatcatca tcaatttaaa cctcatgatt
    26761 taaatgatat cgatctacca gttataatga cagaaaagga tgctgtaaaa tgttattcat
    26821 ttagttcaga taaattatat tatttacccg ttgaagcaaa gttgaatgat tccttttggg
    26881 aagcattttg gtcacatcaa caattacaag gttattacta atatgatgcc aaaacgaatt
    26941 tggttattgc tattatccat gtttttattt ttaagtggca gtgtttttgc agtggaagag
    27001 ccagaggaag acaaagtagc ccaaacgaat gaaccaaccg ttggaagttg ggtatttaca
    27061 ggtatggtca ccaatgaaag cggtgatcga tacggctatt tttttcagat gcaaaggcaa
    27121 ggtactgatt tttatgctaa aaccgcatta attgacggcc aaaccaacca gttaattctg
    27181 ttttatgagg ccaacgaaaa aatagatagc tccactcagt tgaactggca agttgggcgc
    27241 tcatttttgc gctacaatcc gatcaatgac agttggattt ttggcgtgaa agtcgaaaat
    27301 gcaaaaggat ttaattttaa agtagatatg ttaaaacagg ataataaaga tgttgaaaca
    27361 caggtattaa gacctggggt agagttgcaa gcggtacaga ccagtcgatt aaatgggcac
    27421 gtgcaaacgg gagtagataa taaagaacag tttgtaacag gtaataaggc atggttcagc
    27481 aaattatggt ttagcaagga tcaaaattcc cctcatgata taagcactat tttttgcaga
    27541 ttaagtaatg ataatggttt ttattctgca aatctcaagg aacctgatgc aacgggggct
    27601 gcggtagcag gttggcgaga cccttcagga aataatgtca aaatgtctca atttgtttcc
    27661 attaaaaccc taaccgataa tcaatgttta cttagcgtgg gtttgcccag gttgaagtta
    27721 aaattagtca ataccataaa acaagaggat gattttcctc taacagttgc aggcttttct
    27781 gaagaaaatc caaaaggatt ttgttttata tccaaacaaa cttttaagca aatcaaggag
    27841 tcagcagtac aagcggttac taatttggga tagtctaatt tgatttttct ggcaggaggc
    27901 aagtaaataa gcttgccaat agcatggcaa aaggaacaat caaaagggcc agttggtagt
    27961 cagccaggct gtattggctt ttatggttac tgagtacatc taaaaaaaat ccaacgagtg
    28021 gttgaagtaa gggagccgag agcattgcca aggtattggt gaagccagta caagtggaca
    28081 agaattcagg tggggccaat tcattagaaa ttgaataggc tagcatataa gcaccacagg
    28141 ttaaccccat agaaaacatt aaaataccta tcaatagctt attttgagtt ggtaaatata
    28201 aaacaagtat taataataac gcagttgaca gacaagaagc atgaatcagc ggttttcgtc
    28261 tattgagatg taccgaaatt tgacccaata aaggacagct taagccagca cccagtaaga
    28321 ccattgaagt tagtatactg gctgttttga tagtgcaatc cagttttaat tgtaaaaaag
    28381 gaacagccca catggcggca aagacagtaa tgactgagaa ttccagacaa acaaaaaggc
    28441 cattaaacca ggctaattta ttttttagaa tggggataat ttgttgttta tattgatgtg
    28501 gaaaagaaac gactggcttg ctattgggga tacacaacca gcataaactg gcgatcagga
    28561 atccaatgat tcctgctatg ttaataaaat aacgccatcc aaaatggctg atcacagaac
    28621 ccatgccaat cattcctaat actgtaacag taaatcccaa cgtttctgat aggcctatca
    28681 taaaagcgta ttgttttaag ggaaaatgtt ggcgtaacaa atggcttaaa ccaataaagg
    28741 caaaggctga gccagcgcca ataattaacc gcccaaggaa aagagtcggg aggttatgtc
    28801 ccgaggcaaa tacaaaacat ccgatggcac aaagagttgc attgaccgat aacaaatatc
    28861 gggtattgta gcgatcaaaa agaatcccga caggaatttg aaaagaagta tagacatagt
    28921 aaaaggcgct acttaaaagt cctgcgctga gtgcggaaat atgttcttcg tgcattattg
    28981 cgcctacaat gatccccgag gatagttgca agaagaattg gaataataaa aaaaatactc
    29041 ctaccaacca aatggtgatt tttgatttct taatagccga taaattgttc aaagtagttt
    29101 gtattatgtt gaaattgatg gaatatatag cattgttatg tttgtgtaaa tttataatct
    29161 gcaatacctt ttatttcttc atgaattata tttgctatta cattggaatc aataattctg
    29221 tatgtatttt gtaaaatttg aaaaatgatt tctatactgc agatttgtaa tttatgtcac
    29281 aatagatttt ttattattga gaaattatga atttaagaga tttacattat tttgtcatcc
    29341 tggcagatgt aaagcatttt ggtgaagccg ctaagaggtg ttttgtaagt caacccactt
    29401 tgagtatgca aataaaaaag ctggaagaag aattaggtgt tgtgttattt gagcgtacca
    29461 acaaacaggt tttactgact gatcaaggct ccaaattatt agatcgaact agaaaaatat
    29521 taatcctgat tgatgaaatg aaagaacttg ctcgtcagtc ggaagatcta tttacaggtg
    29581 aattgcgttt gggggttata cctaccgtat cgccttacat gttgcccttg gtaatgcccg
    29641 aacttaaaaa tgagtaccct cgcctgaaag tttggttaat agaggatcaa actcataggc
    29701 taattactaa attggagcaa ggagaactgg atgttgctat catggcactt ccaatagata
    29761 agcgtttttc atgtcaaact ttatatgaag aaaagttcta ttttgcttgc gcaaatactc
    29821 atcctttggc ccaagccaaa tcagttaata tcaatgattt aaaaaatcag ccgattatgt
    29881 tgctagaaga agggcattgc ctgagggagc aagccatggc cgtatgccaa tcagcgaaag
    29941 ctgacgacat tgcagatttt actgccacca gtttggaaac cttgagatta atggtacaag
    30001 ctggaatggg cgtcacttta ctgcccgctt tgtctacact aactgcctca actaatcatt
    30061 taaaatgtat tcctttttct gagccggcac cctctcgtat cttggggtta ttttggcgag
    30121 caggaacgcc acgacaagtt tgttttaatg caattgctga attaataaca aaaaatgttc
    30181 aatccaaatt agcatagaaa ttattataaa ttatacgtaa attcctgctt agctggatga
    30241 gctttcatga agctatttta tgaattttaa ctagcattcc taactgagga tgatcaagat
    30301 aataaattgt gcttcctttt aagcgttgtt tttgcgaaac agtaaaagaa gtctcctgat
    30361 tacctggaga caaacactgc aggtcagcat caaatagata gtaattactt tgacgcactt
    30421 tgagggtacc ttgaatttgc catccattat ggttgatttt aggtagcgca accaaactgt
    30481 cacttttcgc tggttgtctc caggaataat gacccaggat ttgaaattgg gatttacggc
    30541 ttaaaagata atgttgatca cttagttttg attgcgatcg cggtagaaga gtgtaaggca
    30601 tatgtttttt attattatcc gaatgcagtt gaatagcatt tggactgaca ggaatgaggg
    30661 gaacaggcga atctgatttt atgcttttaa caccgttttg tggttgtgca aagagtatta
    30721 aatcaatttg gtaacttgat ttggctatcg taagtcccga acataataca ctgattgtta
    30781 ttattaataa tctaatcatg acatttttat ttttgttttt tgtgagcttg gttatctttg
    30841 aggtggcaaa ctcaattttt atgtattata tactcatctg aagggaataa tacatagtag
    30901 agaagctatt acttctattc cacaactatc tttggaaatc tgtcagcata gtttatgggt
    30961 ttcttgctga gctcaattgc agttcgcatc gctgttttaa taaaggtgtc agttaattga
    31021 tttgatgaat gtgttgccgt gggaactccc tcatcgcaat gtctacgtac atggctgtcc
    31081 agcggcagtt gacctaataa tgtacattga tatgcgtcac acagcgcagc tgcaccacct
    31141 ctaccaaaga ttgcttcttg atgaccacaa tggctacaaa tgtgggtgga catattttct
    31201 attattccaa gtacgtcaat gcctgttctg gaaaacatgg ttattgcctt ttgggcatct
    31261 aaagttgcga cattttgagg agtagtaact actatggcag aggttaaagg aattttttgt
    31321 accaaagtga gttgaatatc tccagtgcca ggtggtagat ctatgaataa ataatccaat
    31381 tcattccaaa gagtgatgtc taacatttgg attaaagact tggcgagcat aggacctctc
    31441 caaattaatg cctggtttgt atcggtcaag tagccaatag acatggcctg catgccatga
    31501 gcttcaaccg gtatgtagca attatctttt acttgtacag gttttgtttc accaagcatc
    31561 aatggtatgc ttggtccata aatatccgca tccaaaatgc cgactcgagc acccagtttt
    31621 gccaatgctg ctgcaagatt tactgtgaca gtagactttc cgacccctcc tttgccagag
    31681 gccactgcga tagtattctt tactcctcgc agggctttgc ctgtcaactg agttttatgc
    31741 gcttttatga actgctgaat agaaatagta acttggtaat tggggaattc atcttgaact
    31801 attttctgta ctattggttt atagctcgtt tctaacaaag aggtaggata tccggcagtc
    31861 aagacaacat gcatagcctg gttgctagtt gttattttgt actgtagatt catttctttg
    31921 ccagtaagat caagcaaagg atcctttaat gagccaatta acttaattac tgtatcttca
    31981 atagtcattt tttagcctgg tatgaaaata aataggtaat gataacttag gtctcggcta
    32041 atcactagta ccattactat tcgtttttgc tgggttgatg aatgcatcta cttgagagaa
    32101 atgtattttg ggtatagctt ttctgatgag taagacgaat agcttaagat aatgtcattt
    32161 gattttattg ggcaattgca tagctgctgt gtttttaagc gctcatttta tactgccaat
    32221 caatccaatg tccatttgca agataataca atccacatcg aattgggaat tttgtgcaat
    32281 ctgacagata gcgcccgtac tcatttaatt gttggcgatg cttctgtaag gtgtttatat
    32341 cttctttccc ggttttaaaa tcaatgatcc attgtgtgcc attttcagta aaagtacgat
    32401 cgataatacg ggtaaccaat ttattttcat actcaaccag aagctcatat tcattttgtt
    32461 cattttcatg tctggataaa atccagaggc caattttatt ttcatacatt tgagaaattt
    32521 ggatttttat cattgataga gcagtttgtt gggcttcctg gtttaatcct aatgctttaa
    32581 gttcattatg taccaagttc catggcacat gattaattgt ttggggatgg ttatcacaaa
    32641 tccattgtaa tagttgatgg gacacaatgc ctattagccg aggaataccc gaagatatct
    32701 ctgggcatga actctcttga gattgaacta aactgggaat ctgtttttga taatactcta
    32761 taggtagttt gactaatttc ggcagaggga attcttgttc ttcatgttgg gcttcgtcag
    32821 gatctgcaat gaattcctgg tattttaata agtttcgaaa ggaagattta taggattttg
    32881 ccgaatgatc aagcaaatat aatcttgatt ttgcccgggt aacagcaaca taaagtaatc
    32941 tttgcgcctc ataggcggat ttttcttcat ccaattgatc cagataatcg tacaaagggc
    33001 aattatcctg gtttgcactt tttatgggtg ataccagtaa caagttgcca tggtcttggg
    33061 taggcagctt aagccagcgc aacatgggag attcaccacg atttgcttgt gacccaaggc
    33121 caggtagaat aaccgtatcg aattccagtc ctttggattt atgaatagtc atgatttgta
    33181 accgggaagg ggtggtttgt tgggcataca atttattgaa ttcatttaaa aattcgttca
    33241 tatcaggaat tcgaccttct ttttcatatt ggtctaataa catccataat tgctccagat
    33301 catttaactg agctttatca aggatctttt ctacatgaag attttttaag gtatccgcta
    33361 tccaatcaga tagacgacta tgtgatcgac ataacaaagc ctggttcatg atttgaataa
    33421 aaaaattggc tctaatcctg ccttcttcac ttatatcctg aattttatct aaatggagca
    33481 aattgtgata aattgatttt cttgaattat attgggctat gcagtggatg tcagataatc
    33541 ccaatccaac ataagggctg tggagtatcg ataaccagga caatcgattt cctgggaaaa
    33601 gcagggcatg agcaagtgac cagacgtcac gtaaatgcac taaattggct agtaaatcga
    33661 tgtctgttcc ctgataaggg atattatttt ggcgaagtag gctaatgatt tcagggagtt
    33721 gcgttcttgt cctcactaaa actgcgatag tttgttcagg gttgcggttt aattcactat
    33781 taattatttc aattaaatgc cttgcttcct gttcacgatt tttaaattgc acggcctgaa
    33841 tagtgcttga ttcattcttc tcgatgacat gaacagaagg atggaaagat actgctcctg
    33901 attcgatatc aacttgttga gaaaatattt tactgaaatg atcatttacc caattaacta
    33961 ttgtttctgt cgaacggaaa ttacatctta gttccaatga tttgaggcga actggtccta
    34021 tgccatgctc tttcgcctga aaaaacaatc caacttcagc ctgtctgaaa cggtagatgg
    34081 attgcatagg atcgcctaca aggaataagg tttttccctc tccaggtaac catccttgga
    34141 ctaacttggt aagtaattca aattgtgtaa tggaggtatc ttgaaactca tctattagaa
    34201 gatgatgaat tgaatgatcc agatagagtg ccaaatcagt cggattatcc aaatcaccca
    34261 aagctgccaa ggcttgttga gagatggcag taaaatcaac ttcgttatgt tcaccaaatg
    34321 aaacttgtaa atgtccaacc aataatggca gtaacaaaaa gagtgcttgc aaaacgtccc
    34381 attgctcttt gtcgtattca ggagttggta attgattgac cttaaggagc gcgtccagga
    34441 aatcaggaca atcttgaagt ttagcaaaca aatctgaact ggctgttttt attctggtaa
    34501 attcatttgg tgcacaattt tttttaacta atcccacatg atgatcaaac gatttacgca
    34561 attgattgtc actagttaag agtaatttgc ttaaagcacc tgcaatttgc tgatcgcatt
    34621 tttgaaactc ataccattct cttaatataa atcgtggtga atcaggtttg ttttctataa
    34681 cggctaattc acgcgacaaa agcactagtt cttgagccaa gtccgatggc aggctatttc
    34741 ttagacgaga gagttcatgc tgttcaataa ggcgtaaagc ttgctcaaaa gttgactttt
    34801 cttgttcacg tgccagaaat aatgtcggca accattgatc tctttggcta agaagtgcct
    34861 ggaataattg aattaatcgt tcctgtttat tatcaacatg aagaagcaag gttttaattg
    34921 cttcctggta ctctggcgta gcaatagcat actggataca acaacgtgca gcactaatat
    34981 aatgagaatc gggtgtctcg gaaatgtctg gataagccac ctgtttttcc aataaaggaa
    35041 ttgccctgtt aatgctttgg cacaatgaat caatggttat gatttttaag cgatttggtt
    35101 gctcaagtaa atcccactga tattgattat ttcgctgtaa agcctgtttt gcaaaattta
    35161 aagtgctttg ttgatgagcg ctgacagccg gttgatttga cgcggccata tgtaatgcct
    35221 gaacgattcg ttctctcatt tcacttgctg ctttgcgggt aaaagtcaag gcgataattt
    35281 gttcaggaga ctgcacagtg cttagcaaac gtaaaaagcg ttgggtaaga atctctgttt
    35341 ttcctgaccc ggcaggagcc tggactataa atgattcagc aggatctgtc gcttggctcc
    35401 tttgttcact atcaattaac cgcatgccat tattccagaa ctatttcctg agcaggtggt
    35461 cttaaattgt agtgtgaact catacagtga ccataggctc cagtgtttgc aattaatatc
    35521 acatcgcctt ctctggtaac tggtaataag cggtcataac ccaatgtatc tcccgactca
    35581 cagattgggc caactatatg agcaaacccg gccttttctt catgtagtcg agttaaattg
    35641 acaatttcat gatacgcacc atataaggaa ggccttatca atgagttcat tcctgtttca
    35701 ataccaataa attttacttt tcctttttct ttgcattgcg ttactttggc aagaatgaca
    35761 ccactttcag ctacaaagaa gcgacctggt tcaagccaga ttgctaatcc tggatattga
    35821 gattttactg ccattaattg cgcgtcaagc gcagcaaaat caatgggatg ttggcccggt
    35881 ttttctacaa tacctagacc gccacctaaa ttaattgaac gcacttcagg aaattgatcg
    35941 gttagggagg caagcatcac ggcagtttgt tgccataaat ccgttgagag tatgccacta
    36001 ccagaatgag catgtaagcc tatgacctta atgtggttga ctttggtaag tgatagaatc
    36061 ttgcttatgt cattttgtgt tataccaaat ttggattcat ttccaccagt agaaacgtgc
    36121 ttgtgatggc cagctcctgt accaggatct atgcgaacga ttacttcgcg gtctttaaac
    36181 aagtcaggcc aattttccaa agggtataag ctatcaatag tcacataaca gcccgtttgc
    36241 aaagcaaatt catactcgga tttgggagcg aaattgggtg tgaataaaat tctttctttt
    36301 ttaatattgg gaaacagttt taacaccaat tccaattcct gaatagaaac gcattcaaaa
    36361 cctatccctt ctttttccag agtttttaaa atggttggga atggattcgc tttaatagcg
    36421 taaaacaagc tatcaattga ttccagagca gataattgtc ttgcacgagt tgcctgggtg
    36481 ggactatggt aaacataaca aggagaatgt attgcgctgg ttgtaagcaa gcggtctctt
    36541 tcaatttccc accaaggtgt tgggcgaaca ttgggtttgc caaattcttc atgccagctt
    36601 ttggagtaat aaaagatttg aggattactt tcgattagta gatgatgcaa tctttggcat
    36661 aatttatcgg catgtgattc atcaacaaca aaagtcaaat taaggtcatt cgaggccaga
    36721 gacatcaggt atacttgttt tgcctcaaat acttccaaag ccgggccaag atgaggtaat
    36781 acagttcgga tgtgatgtcc taccaaactg acagcactac agggttctat cagtttggcg
    36841 cgtccaaatt ggtttaaatc gtcgagcaag gcattaattg ctggtctatc atgtattttg
    36901 gcattaacat ccagggatag ggtgacattg aattcagaag aagaaagcaa atcaactgaa
    36961 aacccatgct ttttaaaggc ggcaaataca tcagccagaa atcctacttg ttgccacata
    37021 ttcaaagtgt ctatagaaat tagcagtatg ctgtgcttaa cctggatgga ttttattaag
    37081 ggtgccgatt catcaatgtc tttggtaatc aatgttcctg aatgttcagg caggtgagtg
    37141 tatttgacta ccatagggat attggctttt cgaacaggtg gtatgcaatt ggggtgtaaa
    37201 actttggccc ccattgaggc aatttcctga gcttcatcat aatttaattg ctttaataat
    37261 cgggcgtggg gtaattgatg cgggttggcg gtataaatgc ctgggacatc agtccatatt
    37321 tcacaagaag cggcctgtag ttttcccgca agtagtgctg ctgaagtatc agagcctcct
    37381 cgaccaagca aaacggtttc accatgagga ttggcagcaa aaaaaccttg ggtaataatg
    37441 gcttgagccc cactggagag aaatttttct accaatgccg agtcgtattc actctcacaa
    37501 cgggcagaga gatagttcat tgtttctcca ccaagggttg gtgtgcttgt taacaattct
    37561 ctggcatcat accattttac tttaatccct tgtttttcaa gaaaggcatg gcccaggcgg
    37621 gtcatcatca gctcacccaa acttaaaatt tgcgcatgtg tttttgcagg tgcttgtttg
    37681 agtaaggaga tcccggttag ccattgctgt aattggtgca aatcattagc aattaattga
    37741 tggtttactt ctaattgttc tgccagattc aggtgactgc tctgaatgtc attgaaaagg
    37801 ctatgatgct cgtcaagaag ggctgcctct atggcttttt caagtttgtt ggaaatttga
    37861 gttaaagcag aacagactat aacgggctga acacctgtgt tcagatgttt ttgggtaatt
    37921 gcagcaatat tattccaagt ggctctggtt gaaacactag tgccgccaaa tttgatgact
    37981 acttgttgca tgattagtat tccgctcaaa attaatacac cttagcatgg actaaagtat
    38041 ttgtacaagt agattaagcc ccaatttaaa aaagattttt ctcaagacaa cccaagtcac
    38101 gagttgagtc tacaaagcat gggttgggaa aggtaggctc gaataaaaat ttatcatatg
    38161 cctttgattt taccataatt aactt
//
LOCUS       DACTJE010000012        37209 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.012, whole genome shotgun sequence.
ACCESSION   DACTJE010000012 DACTJE010000000
VERSION     DACTJE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 37209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37209
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.012"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..590)
                     /locus_tag="I9760_000431"
     CDS             complement(<1..590)
                     /locus_tag="I9760_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAT5042226.1"
                     /translation="MEIYKMYIDGKFTLAKSGATRDIFDPANGDLIAKVPESAKEDAI
                     LAIKAARKAFDEGEWRKSLALDRGKLLFKLADLIRANAKMLAELETRNCGKPLLEAEF
                     DVTDAANCFEFYGGLATKIHGETMSVPANSFSYVVREPIGVCGQIIPWNFPLLMAAWK
                     LAPALAAGNTVVLKPSELTPITALELFKLIDECGFPA"
     gene            complement(607..1401)
                     /locus_tag="I9760_000432"
     CDS             complement(607..1401)
                     /locus_tag="I9760_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAT5042227.1"
                     /translation="MATLKINGVDIYYELYGQGKPLVLIAGYCCDHTFWNAMLHELAE
                     QFQVLIFDNRGIGQTRDNGASFTLEAQADDIMAFLEQLGFSNPSILGQSMGGAIAQLL
                     ARKYGKKINKLIILNSVAKFNTRANQAMESLLNLRKENIPFDLLIEAGIPWFFSSEYL
                     AEPKNIAAFKENLKNNPYPQSLQDQARQFRTIPPFDSRGWLHEIKVPTLVIAAEDDIL
                     TLPAESQQLAQGIPNAQFITIPGGHSSPLEQAMIVNEVILKFLTSS"
     gene            1682..3661
                     /locus_tag="I9760_000433"
     CDS             1682..3661
                     /locus_tag="I9760_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042228.1"
                     /translation="MRYTNIELLKRIPQHLKGVMEYYPDVLLFQLNQIQYTHLWHELS
                     SAKYLYTNGFVIKPTHWLMYAFQTVKGWLGFDNHCQPEKISYVLDKLSYYGYTKQFLQ
                     PDFSSITNYSISPEISALVVKSRDDLTTAQLQNKLVNNYFKVEPHLGIDYSYQKLNPN
                     HRFGESWMHAHEWGLIPQLDPQDDSLIAEVISTLDKKGISAKDVFFLQHSKYAKAAAQ
                     YYCDKAKNTIVPSFFFRLLWTDPRPRYLALALAYDPQIAKRDAQKFIEYHLAQKEYDN
                     AFNLLGILNDSNLVLKFLLAIPETERHALIQKDTAIAAIMAKYYIEKKQYLLATQFYT
                     NIEEISPNAAFAIEIQEQNYEKAYDIFKKYNSPNLFSMPERKLLAKIFYSDAETAYVA
                     GKTYRGNKNWEKAKQYYLQSLEQKKAAHHLDPADEYLEDLYAHKRLYALLLIDADIDL
                     NKAEDSDIASIQKAITLLRECRSSNKEEQEHHTRALAAGLMRRVDTLREKIAFNYYSS
                     DFESIRKHKIEHQQDIAILIKTLEELIALLEGTQDKALRLQLGKAHYLLADVQCFFDI
                     NAPDINQHYKMAMKAVPGNPFYVLRVAELFEEEKSKLQKIGITQLKNMGYQVFDFLHW
                     SEERWCKRDDIIHNIKDIHMPPSEPINSSSWNFTF"
     gene            3755..4237
                     /locus_tag="I9760_000434"
     CDS             3755..4237
                     /locus_tag="I9760_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042229.1"
                     /translation="MATYYGQCACGKIQFMCTGEPVFTQYCHCNKCREIASLSQKKQD
                     KSGYSLTAAYLTRDFRILSEDNDFEEIIKEKAKLFLCSYCHSLVYGIALDPAQQDSIG
                     INVNNFSFDTSIPDSFKPVRHIWYASRIMDCDDQLPKFKDAPKEQFGSGELFELPDAN
                     "
     gene            4657..9144
                     /gene="sidE"
                     /locus_tag="I9760_000435"
     CDS             4657..9144
                     /gene="sidE"
                     /locus_tag="I9760_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector NAD-dependent ubiquitin ligase
                     SidE"
                     /protein_id="HAT5042230.1"
                     /translation="MPKYVEGIELTQEGMHAIFERMGHPNITSGTIYNGEPTIDKGAL
                     DRQGFMPVLTGVSPRQDSGHWIMLIKGQGNQYFLFDPLGESSGKYYQNILAKKLPGAT
                     LSVIPNNAGLNMGLCGYWVASVGLRAHAALTQPIPPSLRNLGQTITQEMRDELTQDGS
                     EKITQWLRAVGNEFPDGDIQPDATALRRATEKNVRIDEFQPVLTGTSPKEISINPTAP
                     QEVSVPTWNGFSLYTDETVRNAARYAYDNYLGKPYTGTVEATPVNFGGQMVYRQHHGL
                     AHTLRTMAYAEIIVEEARKAKLRGESLKTFADGRTLADVTPEELRKIMIAQAFFVTGR
                     DDEESSKNYEKYHEQSRDAFLKYVEENKSTLIPDVFKDEKDVKFYADVIEDKDHKWAD
                     SPAHVLVNQGHMVDLVRVKQPPESYLEYYFSQLQPWIGSTATEAVFATQRQFFHATYE
                     AVAGFDSENKEPHLVVDGLGRYVIGQDGNPIREESDDEDEEESGELKFFSQKKKLEEN
                     QRYMRVDEYLKLDEVQKRFPGAGKKLDGGLPGLKEYQYLQRLNSINRARCENDVDFCL
                     GQLQTAHHQTKITPIKRAFQSSSEKARRQPNMDEIAAARIVQQIMANPDCIHDDHVFL
                     NGQKLEEKFFRDLLAKCDMAIVGSLLNDTDIRNIDTLMQHERNTEFHSTDAKAKPVKL
                     GETWEKTIRSGGGVTQIKHDLIFLMQNDAWYHTRVNAIAQNRDKDSTFKEVLITALMT
                     PLTNKSLMDTSRSPAPKTLFRGLDLSEEFKNKLINQAETIIANTTEHLFTDLSTEAFK
                     QIKLNDFSQVSARTCASTSTNIEVPRTIFGSNTIFEILDPDGLLHPKQVGTHVSGSES
                     EYSIYLPEDVALVPIKVSFDGKTGKGKDRHIFTLVAVKSPDFTPRHESGYAVGPLLKM
                     QTPKLEEIQRLVEQAREEPDLERVFNLQSRVARQAKFSTESGYKTFLNEKVAPVLEQS
                     LNGLLDNNVTILGKVLSAFPSDGQWSAFNSVEARQMKIQMDAIKQMVEKKAVLEGQIL
                     PALAQCQNALEKQNIAGALQALRNIPSEKEMQTMLSISGGLRGQIQRAKQDLTETLEP
                     LQRAITAKLVSDQEKVKVRYEKLIAGIPQQIADLEKAELADLAKVKKVVSRFNHLQEE
                     LKLLRNEKIRMHTGSEKVDFSDIAQLEAQLQKIHTKLYDAYLVELTKEISALVKEKPK
                     NLADVKRMVSNFYAMSADIEQLRQEKIKEHGESKDPIDMSDIDKLKEELQKINQFLVK
                     AMGTNIRVSLNQMEVKTFDAQEKEAQQNLKQLDALINKLESSDAVQKQKEELEKLNQL
                     LVEKRKAYPAMVQLQFRSEALIIHLRELCEAHQAQMAKTRNVRAQEITNGRWKVQWLT
                     DWVGLTTDERVTLANKEKELAKFKEDLNNDEYDLQELISNLAEKNPSELEEAIGISKE
                     SAQKLHKLLTHLNHSTTFMSKIEQRLQSIDELLNEFGKQAPRTEMIKTVEEKQGTLLR
                     L"
     gene            9443..11035
                     /locus_tag="I9760_000436"
     CDS             9443..11035
                     /locus_tag="I9760_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HAT5042231.1"
                     /translation="MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATY
                     FLTLQESSTVGIAAGYAMITRKPALINLHNYVGLANAVCNMYNAFTSGIPLLIIAGQH
                     ARPYLIHSPVMSGDLTSLAHTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSI
                     PMDIWQEETQETTIKKTKFLHACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIR
                     LLHTIADRLNADIYAAPYHVREVINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEK
                     VKGYLYTGQSSIPQGIRFIQISAASEHLAFDYPCDLAILGNLEATLTAISKHLKINGL
                     NVPLKPATNIKNLEKKYKQHPLHSLIVTLLNQVNRSVHLITEGSSEDAIVQDIATTLG
                     FLNVNFSPRGGGLGWAMPLAIGISLATRHHSICFVGDGGSMYAIHSIWSAAKYKIPVI
                     FICFVNREYRVLKTDMSQNRSFTEEDFFRLDLNHPPIDVQQIALSFGAQVKTIKSKEE
                     VETVLKSSFAFQGPTFITLYHQ"
     gene            complement(11152..11598)
                     /locus_tag="I9760_000437"
     CDS             complement(11152..11598)
                     /locus_tag="I9760_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAT5042232.1"
                     /translation="MKHNLLDKAYKHCVNHGYRFTEPRERVLKILVDERKPLGAYDIL
                     QRLSMEVDNPKPPTVYRAIQFWHQEGFIHCIDSLKSYVACLHGHHVGQAQFLICNQCD
                     FVKELECVVDFTAVTEAANSIQFSIINCTVEIKGLCSDCNLTNLKN"
     gene            11929..14487
                     /locus_tag="I9760_000438"
     CDS             11929..14487
                     /locus_tag="I9760_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAT5042233.1"
                     /translation="MLYSNYRFYLAKDAQLLNPEESREAIEQILAKTKELELIRVAVD
                     LTSSNKSISLLSASKENHVIKTKVTQILKEQGLSVIDMMEVEVLQDIEKIEHHHPTHK
                     SKNTHLHPVKKHQKKAKHDHSHGHGHHHGHHHENHWIKAALGLIWGIGLLVLSIGTFN
                     IPLVAYYVITGLTSLMTLYLGHTVYQSAWQALKEKKWDTTTLYSISTLTILAVSIASV
                     FVPGLPMMFEAAPLVLGFWHLGEGIEHTLVGKINKKLDVRDCVPPLVLLKGNPDKEIS
                     VKTLIPNDMIIVKKGQVIPVDGVLTQKALLYTTRIDGSPKLKEFHPGDVVKAGMQLAD
                     HIPSLEMQVTKTYQNSYLSLIAENINKANDEKAPIEMFADKVLKYFIPGLIAVALLSG
                     IVIGSLFNPALAIQCVVSVLVSACPCALSLITPMAVKIGMKKASENGIHFNNGKALQA
                     AADIDTVVFDLNGTLTQGKMVVKSLHITDKKYLRPIALLESQSDHPVAKIIKSYMEQQ
                     DTASTEPLEITSIDKSHHSGMKGIINGEVFIIGNKDMLAANGITSIDKPYDNPEKGSV
                     YIVRGTTVIGQIALIDPLREDAIATVKQLKRLGKSVHICTGADQATAEKYAALLGIPK
                     DNICANTVGAVTKKGEVSKTSYIQQLKQKGFKVAMVGDAANDATAIAYSDIGIAVKSS
                     IGDSITEQHAGIVVQQGLLFPIATAFDVAQKTKQNIFQNLFVSLSYNSIITLVAAGLF
                     VALGFALNPVLGVALMVLESTIVLTNLYLFKQQDIVSSTSSNANNAVEIEPAANTTSK
                     VLHALGHRSQPQENLAVVTESKPVNPKVINLFAPKEHSSYRPDATQQKLGCGIC"
     gene            14634..16763
                     /locus_tag="I9760_000439"
     CDS             14634..16763
                     /locus_tag="I9760_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT5042234.1"
                     /translation="MNKTWHQTATGNRSILSNLRLSRTLLNIIFIIALSLLLIINIIS
                     YNQVKNLIFASNWVSHTHEVIQTIDTCLYDIVEIESRQRFYLIRGSSSQLMADIDDMK
                     STLQLNLDKLEQLTRDNPEQNKRIHRFIDLVKQRLNLLNQLVQLKGSGKLDTQEGYDL
                     LNQSQDMSHQVKSLGQEIKSVELVLLSERNTGVVRWAVTSSFILISGGIASILFLTLA
                     FILANIELSTRRKTELQNQNTQIRLKKIIESASDMIAAFGKDQRFITFNEAYQREFKR
                     LFDKSISINMSLEEAFNNAPENKKKLVQSWKESLQRDEETKNIEVNTEQEKTIYEMTS
                     KLIQNGDNEIKGVVHSVRNITKRVQEHTELQESYEKLANGMKELQEKNEQITLLVEMS
                     DIMLACGSQEELSEVMAKYSQRLLQFSSGYLFIMHPSKNYLEKAASWGNPHPHDLTFT
                     PEQCWAIRLGRIHYAGSSRIELMCSHMMFDEQPELSLLCVPLMAQNDIYGLLYLEVGL
                     KFDENQQLLITAFAELTALALANVRLRENLRYQSIRDPLTGLYNRRYLEDFLFKQLHQ
                     AERTKASFAILMLDLDHFKKINDTFGHDAGDLVLKELGQILNNDIRLGDIAARYGGEE
                     FVLLLYDINAQAAKMKAENLRSAISNLQVKYGAQPVGQITASIGISVYPDDAKSPAEV
                     IEAADKALYQAKNKGRNKVILFSEISP"
     gene            16975..17808
                     /locus_tag="I9760_000440"
     CDS             16975..17808
                     /locus_tag="I9760_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HAT5042235.1"
                     /translation="MFSKALISATYKNGKPGQLTEEHEKAEHHRFKTDYLFKNAEISK
                     DLYASRLIQHFLIIKAIETKLQNLSKTEQSEISAFFALSYLEQLWRTTAIENDLRQLN
                     INPDEIHKDEMTKTTENYLENIEKFTPKILLAHFLLHIAGFMHGGNIIRSKYIEPSNR
                     LTTYQITADQYDFSAAISFLSSGKHSPLALYQDMMKQIDNIVLTGDEYQELLEQCKGI
                     YETMSNIYDDLCDMHTHHLKLPTYSLAAISVSLVALGLILKLLMDFLNPMTNPIANAP
                     R"
     gene            17956..19521
                     /locus_tag="I9760_000441"
     CDS             17956..19521
                     /locus_tag="I9760_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAT5042236.1"
                     /translation="MLKRDISTLNILVASAGGMIGSGWLFSPFISAQMAGSNSLISWV
                     IAAIFMLFIALPLCELGTMFPVSGGMSNYPTYTHGREVGFLFAWTSWLSYVVMTPIEI
                     QAILQYSSHFFPILIDNHSESLTLSNYGYLAAVGIMFFVVILNSYGIKFLAECNKYAS
                     VIKFVLPSIAIISLLKQSPSLANVNINLSSKEHWVEIFTALSAGGVAFAFTGFQNGLI
                     LAGEVKNPQRNIPIAILGAVLVGFILYFMLQFSFIAAVPQKYLAQGWHALSYPGDEGP
                     LVGLTLLLGLGIVATLLLFDAAFSPFGTTLVYTAATSRILYGMALNQHLPKFFLKVNR
                     HNIPYVTLYANLLVGSFAFLPFPGWQKLVAFLSSASILSYGIGPICLLAMRKMHPHMN
                     RPFKLANSIFLSHIAFYICNLMLYWCGFSIIWKLNLALLMGLGIYFFYHRYISLRNGS
                     PLVWFIMYMGSFSIISYLGSFGGIGLLKFPFDLICFLPYSILILYMSQVLATPRHDND
                     MIGNVTELPVYNS"
     gene            complement(19692..20798)
                     /gene="ceg7"
                     /locus_tag="I9760_000442"
     CDS             complement(19692..20798)
                     /gene="ceg7"
                     /locus_tag="I9760_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg7"
                     /protein_id="HAT5042237.1"
                     /translation="MSYSIDNPGGGDCGFYALAIGLISIIQNEYKLHGKSKTYDRWKQ
                     VENIYGVRLQDILNIDLTKLHYSPYTYKSELLFLLQMSLRNIAVIVNKEDLVKRIIEE
                     GRSKDQTKIEGSNAYCKFMELVQLYLYRRGSLERISQFNELALSPEIVQTARDIARML
                     RPILRNQPFHQAQKIENAFVKEVLLNDVLSGNTINSNSIILKSLEKIKEQGRWATHDD
                     LKEIAELLKINLNVVGQPNGKPIPEYPTVTLKNLSNTHWITQVESLQGPRQYSTPSKR
                     AFGYETLSDRAVPLKEEQTLDRAFRTETQSERTRRYNQHFETLFDTLSPYGFFPHVQS
                     KRNVHVFGKAQTGLESDEALAAQLQEAELRRILY"
     gene            complement(20981..22378)
                     /locus_tag="I9760_000443"
     CDS             complement(20981..22378)
                     /locus_tag="I9760_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAT5042238.1"
                     /translation="MSCFPSGKNKLVVLCLLLMLSFKCFPIPGKPLLLDAVLKSVDRS
                     YPQIVIARLEIRKAEGDYVNALGKFDPSFDVNPRFQPVGGYINNYADNLFNVPTLYNG
                     LKLFGGYRIGRGDWPIYYQNYLTNSGGEYRAGLSLPLLRDRRIDKERTELFTREETIR
                     MKQQDAAATKIKIYQETIKAYWQWVEAGFQLRTFRHLLNLAKERQNAIIQQAQQGDLP
                     RLAIAENLQLIVQREQLVNQGEMLLAQAGINLSLYYRDAKGKPQKPDESQLPATFPAQ
                     PIRVSNSIAQLQRHPVLRKLENYSKIVKLKQNLARNELLPNLDATAYTFKQYGTGGYP
                     LLIPQAAMIGVNFKFPIFQREARGKLISTTSELQQVQTEMKFTYEQLNNELSNLLVAI
                     RIYQQQVVLLNKELNLARQVEKGETKRFYEGDSTLFLVNQREQTTAQVKLNWINAEIN
                     LQEATALARFFSSTQ"
     gene            complement(22375..23361)
                     /locus_tag="I9760_000444"
     CDS             complement(22375..23361)
                     /locus_tag="I9760_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HAT5042239.1"
                     /translation="MKLHAYSMISKLPKPQKIAKIIFIVFIAIILFLSFTPWQQFALG
                     NGKVIAFSPTERQHTVNSPVSGRIKKWYVDEGMRVKPGDPIVDITDNDPELLSRLEVE
                     KKAILLRIEAAKQAIAAGKANVDRQKKLYEQGINSRRQYELAQIEYAKYQNDLAQANI
                     DKVNVDVRIARQKTQIIKAHVAGIIFRRLTGQESVVVNAGDVLAQILPETESRAVELW
                     VDGNDIPFVRLNQKARLQFEGWPAIQFRGWPEIAVGTFGGTVSFIDPTDNGFGLFRVV
                     IVPNEPWPSVRFLRQGVRVHGWVQLGQVPLWYELWRQYNGFPPESDRENKAS"
     gene            complement(23358..24947)
                     /locus_tag="I9760_000445"
     CDS             complement(23358..24947)
                     /locus_tag="I9760_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5042240.1"
                     /translation="MNAKPINSIIDVLDRQTISSIAMISVIISLLSLSIPIAAQTLVN
                     LIAFGKLLQPVITLSLMVLILMMALGALHIWQIVIIEVIQQKLMVKVSLSLTRHFTHM
                     SLDNFSTHHGPELVNRFFEIVTIKKSLASLLLYGINLGLQLFFGLLLILIYHPLFLLF
                     DIFIILGILLIIFIPYKKALDSAKKECSEKHVVGAWLEEILINRYLFRFSLYHRYVTQ
                     QTDKKLVAFLKARNTHFKQLIKHQIGFYTLSALASSLLLGLGGYLVINNQLSLGQLVA
                     AEIVLGALIYSFRRFGALLENYYDLVASENKIDAVLNLSLEQVKEDEHGELLTPINSI
                     QLEMDSKNKALIAVDKPLLVFSEKSDLCQLFVEQLFGFQDPASLHVLINEIPCARKNL
                     ISLRRFSLLIAEPQWFTGSIYDNLVLNHRNVPNKIIIEQLKNVGLADKIIQLPEGLRT
                     ILYEWQDVFTLVELTKLMLVRALLLQPELIVIDRAFDILNHQEVNEMMELVLSLKNTL
                     LAIVSQRSDFNYLNNRLVISS"
     gene            complement(24989..25840)
                     /locus_tag="I9760_000446"
     CDS             complement(24989..25840)
                     /locus_tag="I9760_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT5042241.1"
                     /translation="MSIDTITLQCFLAVAETQSFTKAAQRVGRTQSAISQQIAKLENL
                     IEKPLINRGRELSLTTDGEIFLGYAKRIYELHRESLDRFKAPELQGEIRFGLPEDFAS
                     IILSDVLVEFSRLHPRVLLNVECDLTLNLIERFNEGKFDLILIKTTQQQVGSESIYVC
                     TEPVEWIGKKELLPLLDKPGPIPLVLSPTPCVYRGNVIDTLDKNHIDWRLVYSSASYA
                     GKMAAVRAGLGITAIQRSMIPNYLERLDYDFLPILNDVHVSLLKKSGSNKAIESLEFF
                     ILQKLKY"
     gene            25942..27462
                     /locus_tag="I9760_000447"
     CDS             25942..27462
                     /locus_tag="I9760_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT5042242.1"
                     /translation="MDKFLNGLSISLMLSPLIAMLFYILLGKTRNIQATYYGTTVIGF
                     GFGLSLLALIVSANTVNQNSYFLFHASTLSLLLSTLILFISFIIHRFSLRYMHGDKCY
                     RRYFFKLSAITLTTVVMALADNLLLFWLAWSASNLLLINLMIHKSQWKAAYNSGILTL
                     KTLLPGSLLLITAFWILFTLNNTLSIETITSEVSNKSSLAIAVALGFIILAAMAQSAI
                     WPFHRWLMSSLNSPTPVSALMHAGLVNGGGFLIVKFAPLFVTQENLLTILFIFGALTA
                     LLGTLWKLLQWNIKSMLASSTMAQMGFMMMQCGLGLFPAAIAHLCWHGLFKSYLFLNS
                     GSAVQQKKYQNKSRPEKLTNLLTAFFGGLSGMYGFALITGKPILSLHASTFLLGFAFI
                     TGTQLTLGLIAYEKHFKKVILALALVFSIGIFYGGSIHLIESLLPNLTGFPQYQLSSL
                     QVLTLIIFFSLWLLFSMGLFKKIRETRLWDWFYMYMFNASTPHPKTITANRHTYYY"
     gene            27477..29765
                     /locus_tag="I9760_000448"
     CDS             27477..29765
                     /locus_tag="I9760_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HAT5042243.1"
                     /translation="MSSHITLIKTNTSNENQMTQCNYPVDNDVMIIQAMVNNVTKQIT
                     PVWPLEKFIACNSLHGFESMSFEEAVIQNQTAKKGTPFNEKLERVNWHMIKWCGSFLD
                     IGQGTIEMPHRDKGLYFGFLKLAPFDSTLHQNNKSTKNWLSNLPEMPEQAIRLCLDKL
                     GVLNQRQEEFLQSTLSHLPGWAGYIKWISEWKNRNGKEENPVSLVDFLAVRLIITCIL
                     WPEANLEEKKKEKDSADTKQLIQNIKHKEDDYRQLLLKKLLPELNKVQIKGNRAKAQM
                     VFCIDVRSEPFRRCIEKLGHYETLGFAGFFGLPVSIKDYDGETIKDSCPVLLKPRFNI
                     HEKAIAANEHCLEHHEKGKEFKKILNRVYQQLKYNFSTPFALVESLGIWCGITMFLKS
                     CSPIFARRLTQDLNEMICPSIQTQPVFELDLLEKEVGISLQEQIAYAEMALRLMGLTD
                     NFAKLVIFCGHGSSTQNNPYASALDCGACGGNQGGKNAQLLASILNKITVRRALAENG
                     INIPQDTVFCGAQHDTTTDEVEIYHSNVSQFIDQDILDQLRADLNMAKHNNNLERINY
                     LNSIDCAEKDIVRRSADWSETRPEWGLARNAAFIVAPRQLTKNIDLEGRCFLHSYDWS
                     KDEDGTLLETILTAPMVVAQWINTQYLFSTIDNVAYGSGSKITHNVAGKIGVMQGNAS
                     DLMHGLPLQSVMSHDEKSFHEPQRLLTVVYAPREIISELVEKHDVLKTLFFNEWVHLV
                     AIDPRSHLFYKLEKTNTWSVIK"
     gene            29779..30132
                     /locus_tag="I9760_000449"
     CDS             29779..30132
                     /locus_tag="I9760_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAT5042244.1"
                     /translation="MNIIKVSEKKEMNISLHPMKKIEVIVSGDNENMVATMMEKANIS
                     GFTLLRNVSGKGHHGFHEGKILFNDKASFVMFIAVAPEEAIAKIALGMKTLFQKNSGV
                     LFVSDVSVARVDYFS"
     gene            30157..30996
                     /locus_tag="I9760_000450"
     CDS             30157..30996
                     /locus_tag="I9760_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042245.1"
                     /translation="MHYQNQRVLLASKHQKEKAIQPPFEEKLGCFIHVPADYDTDQFG
                     TFTGEIPRKASAYETLILKAKAAAKQFGYRYVISSEGSFGPHPQLYFCPADTELMAFV
                     DLDNDLIIAEHEVSTETNYSHIDISATDDYEAFLNKAKFPSHGIIVRALNSEENYIQK
                     GICEPEQLKTTIQKAFTYSTTIRLETDMRAMMNPLRMNVINKLAVKLVHRIQQHCPQC
                     HTPGFGKISAEGHLACISCGTPTELYLRKVLNCLKCEFKTYQARDDGLEFAEQRYCPY
                     CNP"
     gene            31056..31556
                     /gene="purE"
                     /locus_tag="I9760_000451"
     CDS             31056..31556
                     /gene="purE"
                     /locus_tag="I9760_000451"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAT5042246.1"
                     /translation="MKPILIGLIMGSQSDWQTLIHAAHTLDALNIGYEAEIVSAHRTP
                     DKLFRYAEQAEARGLEVIIAGAGGAAHLPGMVAAKTSLPVLGVPVMSQTLNGVDSLLS
                     IVQMPAGIPVGTLSIGKAGAINSALFAAAILANKYPDIRAALKHYREQQTQKVLDNPN
                     PKEEKS"
     gene            31553..32632
                     /locus_tag="I9760_000452"
     CDS             31553..32632
                     /locus_tag="I9760_000452"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAT5042247.1"
                     /translation="MKLGILGGGQLARMLALAAYPLGINTVCLDPSPDACAGQLTRLI
                     VSDFNDELALKQFLSEVDCVTIETENIPFSCAELIAQIKPFYPSTQALKITQDRLYEK
                     NFFRSLNIPTPEFLPVESEGELMQAITDIGMPAVLKTRRFGYDGKGQFVLRAQSDISK
                     SWGLLGSQPLILEELISFESEFSLIAVRNKSGETSFYPLIKNHHRKGILHFSEAPVHQ
                     DTLQQEAQTHAFKILEALNYVGVLTIEFFYQGNQLIANEIAPRVHNSGHWTIEGAHTS
                     QFENHLRALFDLPLGSTEVSGSCFLLNCIGNLLPLEPCLSIPGVHYHAYGKAPRPDRK
                     VGHVTLIDTHLERYAKSKDLLLDIS"
     gene            complement(32718..32930)
                     /locus_tag="I9760_000453"
     CDS             complement(32718..32930)
                     /locus_tag="I9760_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAT5042248.1"
                     /translation="MNNQQKIKCPICDKQNTWRPDNLFRPFCSERCKLIDLGEWASES
                     RKIPGSSIDPESIVTTNNKQDNVDEQ"
     gene            complement(33050..33934)
                     /locus_tag="I9760_000454"
     CDS             complement(33050..33934)
                     /locus_tag="I9760_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT5042249.1"
                     /translation="MEFKLLQTFIEVVRQGSFSKAAENLFTTQSTVSKAIKQLEEELG
                     ITLIERFKKRNVPTTAGEIVYQRGTKLLADRDDLLKELDEIRGLKQGRLRLGISPVGS
                     STLFAPLFVLYRQRYPGIEVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVR
                     SEPLVALLASGHPLATKHCISLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVA
                     QSSQIDFMLELVAAGLGVTFLPRMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYL
                     SEAAQAWLALVREVHVDN"
     gene            34040..34429
                     /locus_tag="I9760_000455"
     CDS             34040..34429
                     /locus_tag="I9760_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAT5042250.1"
                     /translation="MANKIQQNSFFQIVLVGLFWLASELLLKLVKLPLSGGILGLGIV
                     LLLLATNCLKLNRIRRGAELLLANMLLFFIPGVLAILEHREFIGLLGLKILFIILLST
                     ITVMLVTALVVDYCYRWRTHHAKQHSL"
     gene            34407..35114
                     /locus_tag="I9760_000456"
     CDS             34407..35114
                     /locus_tag="I9760_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAT5042251.1"
                     /translation="MPSSILSESMIQAFFWSLITIGMYFITQKLYRRWPFWWLMPLAL
                     TPILVASVVLLLHVNYQDYFRGTKWLVLLIGPATVAFAIPIYEKRALIRQHWPVLLIG
                     VIVGSFTAIASSWGLAILLGLDDNLRLSLLPRSISTPFAIEISRGIGAAPDLTAIFVI
                     VTGILGAILGEFILSRLPFNSALARGALLGVGAHAAGTAQAHKIGNTEGAIASLAMIL
                     VGLFNILLLPLISYLLK"
     gene            35209..36624
                     /locus_tag="I9760_000457"
     CDS             35209..36624
                     /locus_tag="I9760_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAT5042252.1"
                     /translation="MTIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLG
                     QAQAWWLHYSLIALGKSLNQQGLSLVLRKGSPQEIILELVAQYGVESVYWNRCYEPQV
                     IARDKKIKLALVEREIKVQSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPS
                     PFILTKRPRGIEIASDDIGKWKLLPAINWAARFSEYWIPGEEGAQKKLDEFIVHHLSG
                     YKRDRDFPAKNATSRLSPHLHFGEISPWVILRALELAKLEHTCDLASVEHFLSELGWR
                     EFSVYLLYHFPKLDCENFRKEFDAFPWQNDEQLLTCWQKGMTGYPIIDAGMRELWATG
                     YMHNRVRMIVASFLTKDLLIDWRSGAQWFLDTLVDADLANNSASWQWVAGCGADAAPY
                     FRIFNPILQSQKFDPDGVYIRQWIPELAQLNSQSIHQPWTDADASSIFLGKKYPKPII
                     DHQEARTKALSYYQQIKKGMD"
     gene            36657..37130
                     /locus_tag="I9760_000458"
     CDS             36657..37130
                     /locus_tag="I9760_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAT5042253.1"
                     /translation="MNKKSWTELIAWIITFEAIGFFLGMLTQANIPSWYEGLNKSGLT
                     PPGPVFSIVWSILYALLAIVGWVLWCSRNESEMRSVFYLYSVQMLMNWAWTPLFFVLH
                     WTGLSFLWILVMAGLTGLLMFSQTNKKKSLAILLLPYLIWLLFAAYLNGVIWLLN"
BASE COUNT        11506 a         7436 c         7198 g        11069 t
ORIGIN      
        1 gctggaaatc cgcattcatc aatcaacttg aataactcaa gtgccgttat cggggtcaat
       61 tctgatggtt ttaataccac ggtattgcct gcggcaagtg cgggggcaag tttccaggcc
      121 gccataagca gtggaaaatt ccatggaatg atttgaccac acacgcctat gggttcacgt
      181 actacgtaac taaatgaatt cgctggaacc gacatcgttt caccatgaat tttagtcgca
      241 agcccaccgt aaaattcaaa gcaattggca gcatcagtga catcaaactc cgcttcgagt
      301 aacggctttc cgcaatttcg agtttccagt tctgccaaca ttttagcatt ggctcgaatc
      361 aaatcagcaa gcttgaataa caatttgcct cgatcaagcg ccagtgattt tctccactct
      421 ccttcatcga aggcttttct cgctgctttg atagcaagta tggcatcttc tttagcactt
      481 tctggcactt ttgcaatcaa atccccatta gccggatcaa aaatgtcacg tgtagcccca
      541 cttttggcta aagtaaattt cccatcaatg tacattttgt aaatttccat ttattccgca
      601 ccttttttag gaagaggtta aaaattttag tatgacttca ttgactatca tggcttgttc
      661 taatgggctc gaatgccctc ctggaatggt aatgaattga gcattaggga tcccttgtgc
      721 cagttgttga ctttctgcag gcaaggtcag tatatcgtcc tctgctgcta tcactagtgt
      781 gggcacttta atttcatgta gccacccgcg ggaatcaaat ggtggtatgg ttctaaattg
      841 tcgcgcttga tcttgtaagg attgcggata aggattattt ttcaaattct ctttaaaagc
      901 agcgatattt ttaggctcag ccaaatattc gcttgaaaaa aaccatggta ttccggcctc
      961 tattaataaa tcgaatggaa tattctcctt acgcagattc aaaagacttt ccatagcctg
     1021 gtttgctctc gtattaaatt ttgcaaccga atttaaaata atcagcttat tgattttttt
     1081 accatacttt ctggcaagca gttgagcaat agccccaccc attgattgtc ctagaataga
     1141 cgggttgcta aaacctaatt gctccaggaa agccatgatg tcatccgctt gagcttctaa
     1201 tgtaaaagaa gctccattgt ctcttgtttg ccctatcccg cgattgtcaa atatcaatac
     1261 ctggaattgt tcggccaact catggagcat ggcgttccaa aaagtgtgat cacagcaata
     1321 gcctgcaatt aaaaccagtg gtttaccttg cccatagagc tcataataaa tatcaacacc
     1381 attaatctta agtgtggcca tgtcgcaaaa actccttaat cgattttttt gacgaacaaa
     1441 cgcaacgctt cattcaataa tttttcacct gagttaacta tggtttgatc aattaaagca
     1501 tttgtcaaca aacacgttta attagtttct ggtaccataa ttgttgattc tcataaaaag
     1561 cacactacct gacatctagc aagataattg cgagaaaatc agctcgcttc ataattcctt
     1621 aattaaggtt tactatactc cgcttcatct catcttgctc tattagttta gataaaaata
     1681 aatgcgttat actaatattg aattattaaa gagaatccct cagcatctta aaggggttat
     1741 ggaatactac ccggatgtgc ttctgtttca attgaatcaa atacagtata cccatttatg
     1801 gcatgagtta tcttccgcaa aatacctgta tactaacgga tttgtgataa aacctaccca
     1861 ttggttgatg tatgcctttc aaacggttaa aggctggctt ggctttgata atcattgtca
     1921 gccagagaaa atttcatacg ttttagacaa actctcgtat tatggataca caaaacaatt
     1981 tcttcagcct gatttttcat caataacaaa ttactcaatc tctcctgaaa tttctgccct
     2041 ggttgtcaaa agcagagatg atttaacgac agcccaacta caaaataaat tggtgaataa
     2101 ttattttaaa gtggagcccc atcttggaat tgattattcc taccagaaac taaacccaaa
     2161 tcatcgcttt ggtgaaagct ggatgcacgc acacgagtgg ggactgattc cgcaattaga
     2221 ccctcaagat gacagcttga tcgcggaggt aatcagcacc ctggataaga aggggatttc
     2281 agcaaaagat gtgttctttt tgcaacatag caaatacgcc aaagctgctg ctcagtacta
     2341 ttgtgataag gctaaaaata caattgtccc ttcattcttt ttccgcttgc tttggaccga
     2401 cccgcgccct cgctatctcg ctttggctct tgcttacgat cctcaaatag ccaagcggga
     2461 tgcccagaaa ttcattgaat atcatcttgc gcaaaaggaa tatgacaatg catttaatct
     2521 attgggcata ttgaacgact ccaatctggt tttaaaattt ttgttagcga ttccagaaac
     2581 agaacgtcat gctttaattc aaaaagacac agcaatcgcc gccatcatgg cgaaatatta
     2641 tattgaaaaa aaacaatatc tcctggcaac acaattttac accaatattg aagaaattag
     2701 ccctaatgca gcatttgcta ttgaaattca agagcaaaat tatgaaaagg cttatgatat
     2761 ctttaagaaa tacaattccc ctaacctatt ttcaatgcct gagcgtaaat tgcttgccaa
     2821 gatattttat agtgacgctg aaacagccta cgtagccggg aaaacatata gaggaaataa
     2881 aaattgggaa aaagcaaaac aatattatct gcaaagcctg gagcaaaaaa aggcagcgca
     2941 ccacctggat cccgctgacg aatacctgga agatttatac gctcacaaac gcttatatgc
     3001 cttgttatta atcgacgcag atatcgacct gaacaaagca gaggacagtg atattgccag
     3061 catccaaaaa gcaatcactc tgttgagaga atgccgctca agcaataaag aagaacaaga
     3121 acatcatacg cgcgccctgg cagccggact tatgcgccga gtagatactt tgcgtgaaaa
     3181 aattgctttt aattattact cttctgattt tgagtctatc agaaaacata aaattgaaca
     3241 ccaacaagat attgcgattt taattaaaac attggaggag ttaattgctc tattggaagg
     3301 aactcaagac aaagccttgc gcttgcaact gggtaaagca cattatctcc tggccgatgt
     3361 tcaatgcttt tttgatatca atgccccaga cattaaccag cattataaaa tggcgatgaa
     3421 agcagtacca ggaaacccct tttatgtgct acgcgtagca gagctatttg aagaggaaaa
     3481 aagcaaactg caaaaaatcg gcataactca gctgaaaaat atggggtatc aggtcttcga
     3541 ttttcttcat tggtctgaag agcgctggtg taagcgagac gatattattc ataatatcaa
     3601 agatatccac atgccgccct cagaaccaat taatagctca tcatggaatt ttacgtttta
     3661 attaatagct acaaagtata aaataacctc agtttgtgga ttttctttaa aatatacttt
     3721 cgattaccac caattgaaga aggcatttag aataatggca acttattacg ggcaatgcgc
     3781 ttgtggaaaa attcaattta tgtgtacagg tgagcctgtc tttactcaat attgtcattg
     3841 caataaatgt cgagaaattg cttctttgtc tcaaaagaaa caggataaat caggttactc
     3901 cctcactgca gcttatttga ctagagactt caggatcttg tctgaggata atgattttga
     3961 agaaatcatt aaagaaaagg caaagttatt tctttgctca tattgtcaca gccttgttta
     4021 tggtattgca cttgatcccg cgcaacagga tagtattggg attaatgtaa ataactttag
     4081 ctttgatacg agcatcccag attcatttaa accagtaagg cacatatggt atgccagtcg
     4141 tattatggat tgcgatgatc aactgcctaa atttaaagac gctcccaaag agcagtttgg
     4201 ttcaggggaa ttatttgaat tgcctgatgc caactgataa accgtatcct atgtctttat
     4261 ctttccaaaa tattcagcac actacattga tctggctctc atattttttc aatccgacaa
     4321 aacaccgttt tgccaaaact aattcaaaca ggacttacaa aaaaccccaa tttctatgtt
     4381 aaaaaatgtt tttaattcgg tcatttaatt tatctaaact ccctcactaa aaacattttt
     4441 tgccttggcc tcggggtttt tcgtaagtcc tgtcaaataa tttttgtctt ttacatccat
     4501 gataaaaacc aaaggacatt gggatcagat cttaagcagg atgttttttt tatctcttaa
     4561 tattctctta aagtttgaca gctataatat ttacccactg tgctgatttt taagtcacaa
     4621 atattaattt taaagtattt atccaggagt tgtgtaatgc ctaagtacgt tgaagggata
     4681 gaattaaccc aagaagggat gcatgctatt tttgagcgca tgggacatcc aaatattacc
     4741 agcggtacta tctacaacgg agagcctaca attgataaag gtgctcttga caggcaaggc
     4801 tttatgccag tattgaccgg ggtcagccca aggcaagact cgggacattg gattatgtta
     4861 attaaaggtc aaggcaatca atattttctc tttgacccgc tgggagaaag ctcgggaaaa
     4921 tattaccaaa atattttagc taaaaaacta cctggcgcta cgctttctgt tattcctaat
     4981 aatgctggat taaatatggg attatgcggc tattgggttg cctcagttgg tttaagagct
     5041 catgcggcat taacccaacc tattccccct tctttaagaa atctgggtca aaccattacc
     5101 caggaaatgc gagatgaact aacacaagat ggaagcgaaa aaattacaca atggttacgt
     5161 gccgttggca atgaatttcc agatggtgat atacaacctg atgccacagc cttaagacgt
     5221 gctactgaaa agaatgtacg tattgatgag tttcagcctg ttttaactgg aacatcacct
     5281 aaagaaattt cgataaatcc tactgctcct caagaagtat ccgttcccac gtggaatggt
     5341 ttttccttat atactgatga aaccgtgaga aatgcagcca gatacgcgta tgataattac
     5401 ttgggaaaac cttataccgg tacagtagaa gcgacacctg tcaattttgg tgggcaaatg
     5461 gtgtacagac aacaccatgg tcttgcccac acattgcgca ccatggccta tgcggaaatc
     5521 attgttgagg aagcgcgaaa agcaaaatta aggggtgaat cattaaaaac atttgctgat
     5581 ggtcgtactc tggcggatgt cacgcctgaa gaattgagaa aaatcatgat cgcccaggcc
     5641 ttctttgtca ctggaagaga cgatgaagaa tcctccaaga attatgaaaa ataccacgaa
     5701 caaagccgag atgctttttt gaagtatgtc gaagagaata aatccacttt aattcctgat
     5761 gtattcaaag atgaaaaaga tgtcaaattc tatgctgatg tgattgaaga taaagatcat
     5821 aaatgggcag attcaccggc gcatgtcctg gtcaatcagg gccatatggt tgatttggtc
     5881 agagtaaaac aaccccctga atcttacctg gaatactatt tttcacaact tcaaccctgg
     5941 ataggttcaa cagctacgga agctgttttt gcgacacaga gacaattttt ccatgccacc
     6001 tatgaagcag ttgcgggctt tgatagcgaa aacaaagaac ctcatctagt ggtcgatggt
     6061 ttaggacgct atgtgattgg acaagatggc aatccaatac gagaagaatc tgatgacgaa
     6121 gacgaggaag aatcaggtga attaaaattt ttctcacaaa aaaagaaact cgaggaaaac
     6181 caacgttaca tgcgcgttga tgaatattta aaacttgatg aggtccaaaa gcgattccct
     6241 ggcgctggta aaaaactcga tgggggactg cctggactga aagaatatca atatttacag
     6301 cgcttgaact caataaacag agcacggtgt gaaaacgatg tcgacttttg tttgggacaa
     6361 ttacaaacgg cacatcatca aaccaaaatc actccgataa agagagcatt tcaatcgagt
     6421 tctgagaaag cgcgaagaca acccaatatg gatgaaattg ctgctgcacg tattgttcag
     6481 caaatcatgg ccaatcctga ttgtatccat gacgatcatg tcttccttaa cggtcaaaaa
     6541 ctggaagaaa aattctttcg tgatttgttg gcgaaatgtg acatggctat tgtagggtcg
     6601 ctattaaacg acacagacat tagaaatatc gataccttaa tgcaacatga gagaaacaca
     6661 gaatttcact caaccgatgc caaagcaaaa ccggtgaaac tgggagaaac atgggaaaaa
     6721 acaatcagat cgggtggtgg ggttacccag ataaaacatg atttaatttt cctcatgcaa
     6781 aatgatgcct ggtaccatac tcgagtcaat gcgattgcac aaaaccggga taaagattca
     6841 acattcaaag aagtgttgat taccgcttta atgacaccat tgactaacaa atcattgatg
     6901 gatacatcac gttccccggc ccccaaaacg ctattccgtg gattggatct gtcagaagag
     6961 tttaaaaata aattaattaa ccaggctgaa accataattg ccaatacgac agaacatctc
     7021 tttactgatc tatcgacaga agcctttaaa caaatcaaat taaatgactt tagccaggtt
     7081 tctgctagaa cttgtgccag tacttccact aacattgaag ttcccaggac aatctttggc
     7141 tccaatacca tctttgaaat acttgatcct gatggtttat tacaccccaa acaagttgga
     7201 acccatgtat cagggtcaga aagtgaatat tccatttatt tgccagaaga tgttgccttg
     7261 gttccaatca aagtaagctt tgatggaaaa acaggtaaag gcaaagaccg acatattttt
     7321 actttagttg cggtaaaaag ccccgatttc acgccaaggc atgaaagtgg ttatgcggtt
     7381 ggtcctcttt tgaaaatgca aacgccaaaa ttagaagaaa ttcaaagatt agttgagcaa
     7441 gcaagggaag agcctgacct ggaaagggta ttcaatttac aatcgagagt agcaagacaa
     7501 gccaaattct ccactgaatc aggatataaa acgtttctga atgaaaaggt ggcgcctgtg
     7561 ttggaacaaa gcctcaatgg cctcttggac aataatgtaa ccattctagg caaagtatta
     7621 tcagcattcc cttcagacgg ccaatggtct gcctttaatt cagttgaagc aaggcaaatg
     7681 aaaatacaga tggatgccat taagcaaatg gttgaaaaga aagccgtcct cgaaggtcaa
     7741 attctccctg ccttggctca atgccaaaat gctttggaga aacaaaatat tgcaggtgca
     7801 ttacaagcac ttagaaacat cccttctgaa aaagaaatgc aaaccatgct gtcaattagt
     7861 ggtggtttga gaggacaaat ccagcgtgcc aagcaagatt tgactgaaac tctggagcca
     7921 cttcaacgcg ctataactgc taaattggta agcgatcaag aaaaagtgaa agtgcgttat
     7981 gaaaaactga tagcaggcat acctcaacag atcgccgatc ttgaaaaagc agaactcgct
     8041 gatcttgcca aggttaaaaa agtggtctct cgttttaatc acctgcaaga agaattaaaa
     8101 ttattgcgta atgaaaaaat tcgcatgcac actggctctg aaaaagtcga tttttcagat
     8161 atcgcacaat tagaggcgca actacaaaaa atccatacta aattatatga cgcctatttg
     8221 gttgaattaa ccaaagaaat ttcagcactg gttaaagaaa aaccgaaaaa cctggccgac
     8281 gtgaaaagga tggtttcgaa tttctacgct atgtcagctg atattgaaca attgcgtcag
     8341 gaaaagataa aagaacacgg tgagtcaaaa gatccgattg atatgtctga tattgataaa
     8401 ttaaaggaag aactacaaaa aattaatcaa tttttagtga aggcgatggg aactaatatc
     8461 agggtttcac tgaatcaaat ggaagtaaaa acttttgatg cccaggaaaa agaagcacaa
     8521 caaaatttga agcaactaga cgcactgatt aataaattgg aaagttctga tgctgtacag
     8581 aaacaaaaag aagaactcga aaaattaaac caattgcttg tcgagaagcg aaaagcctat
     8641 cccgcaatgg tgcagttgca attcaggagt gaagcattaa ttatccattt acgtgaatta
     8701 tgtgaagctc atcaagctca aatggctaaa acaagaaatg taagggcgca agaaatcact
     8761 aatggacgat ggaaagtaca atggttgact gattgggttg gtttaactac agatgagcgg
     8821 gtaactcttg ccaataaaga gaaagaattg gctaaattca aggaagattt aaataatgac
     8881 gagtatgatt tgcaggaact catcagtaac ctggctgaga aaaacccgtc agaattggaa
     8941 gaagccatag ggatatctaa agagagcgct cagaaattgc ataaactgtt aactcatcta
     9001 aaccacagta caacatttat gagtaaaatc gagcaacgat tacagtctat tgatgagcta
     9061 ttaaacgagt ttggcaagca agcgccccga actgaaatga taaaaactgt tgaggaaaaa
     9121 caaggaactc tcttaagatt gtaattcttt atcttccacg gtagcccatt aagggctacc
     9181 ccgtgggata ttttagaaga ttacggaaaa taagattata attatcacct cttgccagat
     9241 ttaaaatgca aggatatatc taatcgctgc catctaattt atttttgctc gcaatacccc
     9301 atcactacat ttaccacagc attagctcct aaaaaccgta tttttgccct attttttatg
     9361 agtgagcgaa attgaacaaa tagctctctt gtgctaggaa tataaatatg gaaagcgtta
     9421 ctatttctaa ggatgcttat ctgtgaaaaa aacaggaagt gatgttctca aagaatttat
     9481 aacgaccttt gaaattaatt atatttttgg caaccccggc actacagaat tgaaatttct
     9541 ggaagcgatt gagcagtgtg ataatgccac ctattttctc acactacaag aatccagtac
     9601 cgtaggtata gccgcaggct atgccatgat caccaggaaa ccagctctta ttaatttaca
     9661 taattatgtt ggcttagcga atgctgtctg taatatgtac aatgcattca cttcaggaat
     9721 tcccctgcta ataattgctg gccaacatgc acgtccttat ttaattcata gcccagtcat
     9781 gtccggagac ttgacctctt tagctcacac tgcagtaaaa tatgcctatg aggtgaattc
     9841 cgccagcgat ctggccgttg ctttacagcg tggttatgct caggcaattt tgcctgggcc
     9901 aggtccggtt tttctatcaa tccctatgga catttggcaa gaagaaactc aagaaaccac
     9961 gataaagaag acaaaatttt tacatgcttg catcaacgct gaaatagagc aaatttgtga
    10021 ggttttaaca aataccccca aaggaaaatt ggcatttata gcggattatg aggtaggtgc
    10081 atcgaatagt attcgccttt tacataccat tgccgatcgg ctaaatgccg atatttatgc
    10141 tgccccctat catgtgcgtg aagtaattaa tcccttaagt gtaaattaca aaggtagatt
    10201 gtcagataaa tctggagaag tgcacaacct gctgctgcaa tatgaaactg tggtactttt
    10261 aggcgaaaaa gtgaaaggat atttgtatac tggtcaatct tctattccac agggtatacg
    10321 attcatacaa atcagtgccg catcggagca tctggccttt gactatcctt gcgatctggc
    10381 aattcttggc aatcttgagg cgaccttaac tgctattagc aagcatttaa aaattaatgg
    10441 gctaaatgtg ccgcttaaac cagccaccaa cattaaaaat ttggaaaaaa aatataagca
    10501 acatcctctg cattcgttga ttgttaccct cctgaatcaa gtaaatcgct ctgttcattt
    10561 aataacagaa ggatcatcag aagatgctat tgtgcaggac attgcaacaa cactaggttt
    10621 tttaaatgtg aatttttcac caagaggggg tgggcttggc tgggctatgc cattagcaat
    10681 aggtatttct ctagcgacca gacaccattc aatttgtttt gtaggagatg ggggcagcat
    10741 gtatgcgatt cattcaatat ggtcagctgc caaatataag attcctgtca ttttcatttg
    10801 ttttgttaat cgagaataca gagtattaaa aacagatatg tctcaaaata gatctttcac
    10861 agaagaagat ttttttcgcc tggatttgaa tcatccacca attgatgttc aacaaattgc
    10921 cttatctttc ggggcacagg tgaagaccat taaatcaaaa gaagaagtag agactgttct
    10981 caagagtagc tttgcattcc aaggcccaac atttattacc ctatatcatc aataaaacat
    11041 ttcgaaagcc agacattggc ttttgcatta tctaaaaatg taaagcttaa aatcagtctc
    11101 attatttaaa aatttttatt ttacaactca taggaaaatt attgccggga attaattttt
    11161 caggttagtc agattgcaat cagaacacaa gcctttgatt tctacagtac aattaataat
    11221 tgaaaattga atggaattcg ccgcttctgt taccgcagta aaatcaacta cacattccaa
    11281 ttctttgaca aaatcacact gattgcatat aagaaattgg gcttgtccca catgatgccc
    11341 atgcaaacag gcgacatagg atttcagact gtcaatgcaa tgaataaatc cctcttgatg
    11401 ccaaaattga atagctcggt agaccgttgg tggcttgggg ttatccactt ccatagataa
    11461 tctttgcagt atgtcataag ccccgagtgg ctttctctca tcgactaata ttttgaggac
    11521 acgctccctt ggctcggtaa atcgatagcc atggtttaca caatgtttat aagccttatc
    11581 caacaaatta tgtttcatta atcttcttac aagcgaatag ttagtgcgca ctattttatc
    11641 ataatccctg ttgcaaggaa aaatgatcaa acaacagcca atcattattt catagaaatc
    11701 atttactgtc taaaaattat ttaatttttc ttgttatcta acaattaaat gttacaatat
    11761 aacatttttt gagaaaggca gtatgtgctt gtaacgcctg gcttaggaag aaatcttatt
    11821 agggacaaac cgctggctct attgtgcaga cccagtttca aaataggcat ttgcagaaaa
    11881 ttaacctttc tctatagcaa ccagaacccc tttaaataga ggaaagccat gctgtatagt
    11941 aattatcgat tctaccttgc aaaagatgcc caacttctca acccggaaga atccagagaa
    12001 gcaattgagc agattttagc taaaactaaa gagttggagt taataagagt agctgtcgat
    12061 ttgactagtt caaataaaag catcagctta ttgagcgcat ccaaagagaa tcatgtcata
    12121 aaaaccaaag tgacccagat tttaaaggag cagggccttt ctgtaataga catgatggaa
    12181 gtggaagtgc tccaggatat tgaaaaaata gagcatcacc atcctactca taagtctaag
    12241 aacacacatc ttcatcccgt taaaaagcac caaaaaaaag caaaacatga ccattctcat
    12301 gggcatgggc accaccatgg acatcatcat gaaaatcatt ggatcaaagc agcattaggt
    12361 ttgatttggg gaattggatt acttgttctt tctattggga cttttaatat tcccttggtt
    12421 gcttactatg tcattacagg acttacctct ttgatgactt tgtatttggg ccacacagtt
    12481 tatcaatcag cctggcaagc attgaaagaa aaaaaatggg acaccactac tttatactca
    12541 attagcactc taactattct tgctgtttct attgcgagcg tttttgtccc tggcctaccc
    12601 atgatgtttg aggcagcccc tctggtttta ggcttttggc atttaggtga aggaattgaa
    12661 catactctgg tcggtaaaat caataaaaaa ttggatgtcc gggattgtgt accaccatta
    12721 gtcctgttaa aaggtaatcc ggataaagag atttcagtca agacattaat tcctaacgac
    12781 atgattatcg ttaaaaaagg acaggtcatt cctgttgatg gtgtcttaac gcaaaaagcg
    12841 ctcctttata ccacaagaat tgatggctcg cccaaactca aagaatttca ccctggggat
    12901 gttgtaaaag ctggcatgca attggcagat catattcctt ctctggaaat gcaggttacc
    12961 aaaacttatc aaaattctta tttgtcattg attgccgaaa acattaataa agcgaatgat
    13021 gagaaagcgc ctatcgaaat gtttgcagac aaggtattga aatattttat tcctggtttg
    13081 attgcggtag cgctcctatc ggggattgtg atcggttcgt tgtttaatcc agcactggct
    13141 attcaatgcg tcgtgtctgt tctggtaagt gcctgcccgt gcgctttgag tttaattacc
    13201 ccaatggcag ttaaaatcgg tatgaaaaaa gcctctgaaa acggcatcca ctttaacaac
    13261 ggtaaagcct tgcaagcagc agccgacatc gataccgttg tttttgattt gaatggcact
    13321 ctgactcagg gcaaaatggt ggtgaaatca ttgcatatta ctgataaaaa gtatttgcgc
    13381 cctattgcat tactggaaag ccagtcggat catccagtag cgaaaattat taaatcttac
    13441 atggaacagc aagatacggc ttcaaccgaa ccattagaaa taacgtcaat tgataagagt
    13501 catcattccg gcatgaaagg tattattaat ggtgaggtct ttattatagg gaataaagac
    13561 atgcttgccg ccaatggaat tacctctatc gacaaaccct atgataatcc tgaaaaaggt
    13621 tctgtttata ttgtgcgtgg gacaacggtg ataggtcaaa ttgccttaat tgatccattg
    13681 cgagaggatg ccattgctac agtgaaacaa ttgaaacgtc ttggaaaatc cgtacatatt
    13741 tgcaccgggg cagatcaagc tactgcagaa aaatatgcgg cattattagg cattcccaag
    13801 gataatatct gtgcgaacac tgtgggggct gtcacgaaaa agggggaagt atccaaaacc
    13861 agttatattc aacaattaaa gcaaaaaggc tttaaagtag ctatggttgg tgatgccgct
    13921 aatgacgcta cggcaattgc ctattccgat ataggcattg ctgttaaatc aagtatcggt
    13981 gactccataa ccgaacaaca tgccggcata gtcgtacaac aaggcttatt atttcctata
    14041 gcaacagcat ttgatgtagc ccaaaaaacc aaacaaaata ttttccaaaa cctgttcgtg
    14101 agcctaagct ataactcaat aattacgctt gtggcggcgg ggttgtttgt ggcattaggt
    14161 tttgcactaa atcctgtgct gggtgtcgca ttgatggtgc ttgaatccac tattgttttg
    14221 acgaacttat atcttttcaa gcaacaagat attgtgtcct caacatcatc caatgccaat
    14281 aatgctgtag agatcgagcc cgctgcaaat acaacctcaa aggtcttgca tgctttaggg
    14341 catcgttccc agcctcaaga aaatttagca gttgtgacag agtctaaacc agtcaatccc
    14401 aaagtaataa atttgtttgc acctaaagaa cattcttcgt ataggcctga tgccactcaa
    14461 caaaaattgg ggtgcggcat ctgttgatca agcagtactg gaaattgcct tgattttcta
    14521 cttcaaggca gttgttacga tagccatcat ttcgttaata actgtttttt ggactatact
    14581 cttatcaagg ccatgcaaag catcaagcca taaaattagg atgattcaat gccatgaata
    14641 aaacttggca tcaaaccgcc actggaaacc gctccatttt aagtaatttg cgattaagca
    14701 gaactctact taatatcatc tttattattg ccctttctct gcttttaatc atcaatatta
    14761 tttcttataa ccaggtcaaa aatttaatct tcgccagtaa ctgggtttct catactcatg
    14821 aagtcatcca aaccatagat acatgccttt atgatatagt agaaattgaa tctcgccaga
    14881 gattttactt gattcgaggt tcaagctctc agcttatggc tgatatagat gatatgaaat
    14941 caacattaca actgaattta gacaaacttg agcaattaac cagggacaac cccgaacaaa
    15001 acaaacgaat ccatcgtttt attgatttgg ttaaacaaag attaaatttg ttaaaccaac
    15061 ttgtacagtt aaagggaagc ggcaaactcg atacacaaga aggttatgat ttacttaacc
    15121 aaagtcaaga catgtcacat caagttaaaa gtcttggaca agaaatcaaa tccgttgaat
    15181 tggtgttgct atcagaaagg aatactggtg tggttcgctg ggcagttacc agtagtttta
    15241 ttttaatatc cggaggtatt gcaagcatcc tgtttttaac gctcgctttt attctggcaa
    15301 atattgaatt gtcaacacgc agaaaaactg aactacagaa tcaaaatacg caaatacggc
    15361 tgaagaaaat tatagaaagt gccagtgata tgatagcagc ttttggcaaa gaccagcgat
    15421 ttattacttt taatgaagct tatcagcgtg agtttaaacg tctttttgat aaatccataa
    15481 gcatcaatat gtctctggaa gaagctttca ataatgctcc tgaaaacaaa aaaaaattag
    15541 tgcaatcctg gaaagaatct ctacaaagag atgaagaaac caaaaatatc gaagtgaata
    15601 cagaacagga aaagactatt tatgaaatga cctccaaact catccaaaac ggcgacaatg
    15661 aaattaaagg tgtggtacac agcgttcgca acattaccaa acgggtgcaa gaacatactg
    15721 aactacaaga gtcgtatgaa aaactggcta atggaatgaa agaactgcaa gagaaaaatg
    15781 aacaaattac tctgctggtt gagatgagtg acatcatgct ggcttgcggc tcccaggaag
    15841 agctaagtga ggtaatggcg aaatactccc aacgactact gcaattttca agcggctacc
    15901 tgtttatcat gcatccttcc aaaaattatt tggaaaaagc agccagttgg ggtaatcctc
    15961 acccgcacga tctgactttt accccagagc aatgttgggc tatccggctt ggtcgaattc
    16021 attatgcagg ttcatctcgt atcgaattga tgtgtagtca tatgatgttt gacgagcaac
    16081 ccgagttgtc tctgctttgt gtacccctaa tggcccaaaa tgacatttac ggcttgttat
    16141 accttgaagt tggcctaaag tttgatgaaa accagcaatt actaataacc gcattcgcag
    16201 agcttaccgc cttggctttg gcaaatgtcc gccttagaga aaatttacgc tatcaatcta
    16261 tccgtgatcc actcactgga ttatacaatc gccgttatct ggaagatttt ctatttaaac
    16321 aattacatca ggctgaacga acaaaagcct catttgccat tttaatgctg gatttggatc
    16381 attttaaaaa gatcaacgat acctttggtc atgacgcagg agatcttgta ttaaaagagc
    16441 tggggcaaat tttaaacaat gatattcgcc tcggtgatat tgcagcccga tatgggggag
    16501 aagaatttgt cctgctgctc tatgatatta atgctcaagc cgctaaaatg aaagccgaaa
    16561 atttgcgttc ggccatttcc aatttacagg ttaaatacgg agcacaacct gttgggcaga
    16621 taacggcctc aatcggaata tccgtctatc ctgacgatgc caaatcacct gctgaagtga
    16681 ttgaagcagc tgataaggct ttataccaag ccaaaaacaa aggcaggaat aaggtaattt
    16741 tattctcgga aatatcaccc tgattttgat tgcgactcct gacctaaatg ttgtggtgtt
    16801 tcttatgtcc gatagagtgt ttcatgtttt atatgagttt tgagagcaaa acaatagata
    16861 ctatactgca tagcatccgg tatttttaat agcaacccgg cctacgataa tatataatgc
    16921 gcagccatca ggcataatta ctcgtatcca tgctctataa aggaattatc tcgaatgttt
    16981 agcaaagccc taatcagtgc aacctataaa aatggaaaac ccggccaact tacagaagaa
    17041 catgaaaaag cagagcatca ccgctttaag acagactatc tgtttaaaaa tgcagagatc
    17101 tctaaagact tatatgcttc acgcttgatc cagcattttt tgatcattaa agccatagaa
    17161 acaaaattac aaaatttgtc aaaaacagaa cagtcagaaa tcagcgcatt ttttgcttta
    17221 tcttatcttg agcaattatg gcgtactacc gctatagaaa atgatttacg acaactaaac
    17281 attaatcctg atgaaattca taaggatgag atgacaaaaa caaccgaaaa ttaccttgaa
    17341 aacatagaaa aatttacacc caaaatactt ctcgctcatt ttttattaca tattgccggg
    17401 tttatgcatg gcgggaatat tatccgctcg aaatacattg aaccaagcaa tcgcttgacc
    17461 acctaccaaa taacagccga tcaatatgat ttttctgctg ccatctcctt tttatcctcg
    17521 ggaaaacatt caccgctcgc tctttatcag gatatgatga aacaaatcga taacattgtt
    17581 ctaactggtg atgagtatca agagcttctt gagcaatgca aaggcatcta tgaaaccatg
    17641 agcaatatct acgatgattt atgtgatatg cacactcatc acctgaagct gcccacttac
    17701 tcacttgcgg ctatcagcgt cagtctggtt gcacttggat taattctaaa actattgatg
    17761 gatttcttaa atccaatgac gaatcctata gcaaacgccc ctcgttaagg ctcatcgtca
    17821 gaatgttatt gtggtctaga tattaagatt tacaatgatt tatttcttta tatataatca
    17881 ttacccactt ttatagaaat ttgaacaata tcgaacagat agtgttgttt tgataaacaa
    17941 atggaacagt aacagatgtt aaaacgggat atttcaaccc tgaatatatt agttgcatca
    18001 gctggtggaa tgattggttc aggttggctg ttcagcccat ttattagtgc ccaaatggca
    18061 ggcagtaatt ctctgataag ctgggttatt gcagcaatat ttatgttgtt tattgcccta
    18121 ccgctttgtg aactgggaac gatgtttcca gtttccgggg gtatgtctaa ttatcctact
    18181 tacacacacg gtcgagaagt gggtttttta ttcgcatgga catcctggtt atcttatgtt
    18241 gttatgacac ctattgagat acaagcaatt ttgcaatatt caagtcattt ttttccaata
    18301 ttaatcgaca atcatagcga atcactcaca ctgtccaatt atggttatct tgctgcagtt
    18361 gggattatgt ttttcgtagt tattttgaat tcttatggga ttaagttttt agccgagtgt
    18421 aacaagtatg ccagcgtcat taaatttgtt ttgccgagta tcgctattat ctcactgtta
    18481 aagcaatcac cctctcttgc taatgttaat attaatctgt caagcaaaga acactgggta
    18541 gagatattta ccgctctttc tgcaggcggg gtagcttttg cttttacagg gtttcaaaat
    18601 ggtttaatcc tggcaggaga agttaaaaat ccacaacgaa atattcctat tgccatactt
    18661 ggcgcggtat tggttggctt tattctttat tttatgttgc agttcagttt tatagcggcg
    18721 gtaccacaaa agtatttggc tcaaggttgg cacgcattga gttaccccgg tgatgaaggt
    18781 ccattggttg gattaactct tttgttagga cttggcattg ttgctactct gttattgttt
    18841 gacgccgctt tttctccttt tggcacgact ttagtttata ccgcagctac atctcgtatt
    18901 ttgtatggca tggcattaaa ccaacatttg cccaagtttt tcttaaaggt caaccgccat
    18961 aacatacctt acgtaacact ttatgcaaat cttttggttg gttcttttgc attcttaccc
    19021 tttccgggat ggcaaaaact agtggcattc ctttcatcag caagtatttt atcctacgga
    19081 attggcccga tttgtctctt ggccatgcgt aaaatgcacc cccatatgaa tagacccttc
    19141 aagctcgcaa atagtatttt cttgtcacat atcgcctttt atatatgtaa tctcatgttg
    19201 tactggtgtg gtttttctat tatttggaag ctaaacttag cattgcttat ggggcttggt
    19261 atttactttt tttaccatcg ctatatttct ttgagaaatg gctcgcctct ggtgtggttt
    19321 atcatgtaca tgggatcatt ttctattatt tcttatttag gatcttttgg cggcattggt
    19381 ttactgaaat tccctttcga tttaatctgc ttcttgcctt atagtatttt aatactttac
    19441 atgtctcaag ttttagcgac accccgtcat gacaatgaca tgattggtaa tgttacagaa
    19501 ttgccagtat acaattcttg aggtagcaaa gcgacaaagt aaaaacgctt cctgattatc
    19561 cttgtctgtt ttgtagttca ctgtcatgac ttcttatggg taaaatgcat caagggtaac
    19621 ttgcctgctt cctgccaagg taccctgatg catattgagt tcccaacatt ttttacgata
    19681 aagcgatcgc atcagtataa gattcgtcta agttccgctt cttgtaattg agctgccaaa
    19741 gcctcatcag actccaaacc cgtttgcgct ttaccaaaaa cgtgaacatt tcttttactt
    19801 tgaacatgcg gaaagaaacc gtaaggtgat aaagtatcaa acagtgtttc aaagtgttgg
    19861 ttgtaacgtc ttgttctctc ggattgagtt tcggttctga aggccctgtc aagagtttgc
    19921 tcttctttta aaggaacggc tctgtcagat agagtttcat aaccaaaagc tcgcttactg
    19981 ggtgtgctgt attgtctggg tccctggaga gattctactt gtgttatcca atgtgtatta
    20041 ctcaggttct ttagagtgac cgtaggatat tccggaatag gtttgccatt cggttgtcca
    20101 acaacgttga ggtttatttt aagtagctcg gcaatttctt tcaaatcatc atgcgttgcc
    20161 catcttccct gctctttaat tttttcaagg ctctttaaaa taattgaatt tgaattgatt
    20221 gtattgcctg atagaacatc gtttagcaag acttccttaa caaacgcatt ttctattttc
    20281 tgtgcctgat gaaaaggttg attcctgagg attggtctca gcattctagc gatatctcgc
    20341 gctgtttgaa cgatttccgg ggagagggct agttcattga attgacttat tctttccaga
    20401 gaaccccttc gatacaaata aagttgaacc aattccatga atttgcaata agcatttgat
    20461 ccttcgattt tggtttgatc tttacttcgc ccttcttcga ttattctctt gaccaaatct
    20521 tctttattca caatcacggc aatatttcgt aaagacatct gcaatagaaa taataattca
    20581 cttttataag tgtagggtga ataatgcagt tttgttaaat cgatgttaag gatgtcttgc
    20641 agtcgtacgc cataaatatt ttcgacttgt ttccatcgat cataagtttt actttttccg
    20701 tgaagtttgt attcattttg tatgatgctt attaaaccaa tagcaagagc ataaaatcca
    20761 caatccccgc caccaggatt atctattgaa tatgacatct gtatactccc ttttaggatt
    20821 aatgatttaa tgtttcagtc aagcattata aaatatgaac attaagtaaa cattatgtaa
    20881 catatgccaa gggggagtgc aagtatacaa taattatctt ttgcctcgcc tgtgggtaag
    20941 caagctattt atcaattggg tctccaattg atatccgtta ttattgcgtg gaagaaaaaa
    21001 atctcgctaa agccgtagcc tcttgtaaat tgatttctgc attaatccaa tttaacttga
    21061 cttgcgctgt tgtttgttca cgttgattca ctaaaaaaag cgtgctatca ccttcataaa
    21121 atctcttggt ttcccccttc tctacttgcc gtgcaagatt taactcttta ttaagcaaaa
    21181 ccacttgctg ctgatatatt cttattgcaa ccaagagatt ggacagctcg ttatttaact
    21241 gttcgtaggt aaacttcatt tccgtttgta cttgctgtaa ttcgctcgtg gtgctgataa
    21301 gctttcctct tgcttcacgt tgaaaaatag gaaatttaaa attcacgcct atcatagcag
    21361 cctgagggat caataaagga taaccgcctg ttccatattg tttaaaggta taagctgtcg
    21421 catcaagatt aggaagaagc tcatttcttg ctaaattttg ttttaattta acaatttttg
    21481 aataattctc caatttacgc aacactggat gccgttgtaa ctgtgcaata ctattggata
    21541 ctcgaattgg ttgggcagga aatgttgccg gtaattgact ttcatccggt ttttgcggtt
    21601 tgccctttgc gtcacgatag taaagtgaaa gattgatgcc agcctgtgcc aataacattt
    21661 ctccttgatt gaccagttgt tcacgttgaa cgataagttg taaattttct gcaatggcaa
    21721 gtctaggcaa atcaccttgt tgagcttgtt gaataatcgc attttgccgc tctttcgcta
    21781 aatttagcaa atgcctgaaa gttcttaatt gaaaacccgc ctcaacccat tgccaatagg
    21841 ctttaatagt ttcctgataa attttaattt ttgtagcagc agcatcctgc tgtttcatgc
    21901 gaattgtttc ttctcttgta aaaagctcgg tacgctcttt atcaattcgc ctgtctctca
    21961 acaaaggtaa agaaaggccc gcacgatact caccaccaga gttagtcagg taattttgat
    22021 agtaaatggg ccaatcacct ctaccaatac gatagcctcc aaacaattta agtccattat
    22081 agagggttgg cacattaaaa agattatctg cgtaattatt aatatacccc ccgaccggct
    22141 ggaagcgggg attcacatcg aaagaggggt caaattttcc taatgcatta acataatcac
    22201 cttccgcttt tctgatttca agacgcgcta tgactatctg agggtaactt cgatcaacac
    22261 ttttaagtac ggcatcaagc agcaaaggtt tgcctggtat tggaaaacac ttgaatgaca
    22321 acatcaagag caaacataag acaaccaatt tgtttttgcc tgaaggaaaa caactcatga
    22381 tgctttattc tcgcgatcac tttccggtgg gaaaccatta tactgacgcc aaagctcata
    22441 ccataaagga acttgaccca attggaccca cccatgcacc cgcactcctt gccgtaaaaa
    22501 acgcacacta ggccatggct cattaggaac aatcactacg cggaataaac caaacccatt
    22561 atctgtagga tcaataaagg agactgtacc tccaaatgtt ccgactgcaa tttctggcca
    22621 tccgcgaaat tgaatagcag gccatccttc aaattgcaaa cgggcttttt gattaaggcg
    22681 tacgaaaggt atatcattgc cgtcaaccca taattcaaca gcgcgagact ctgtttcagg
    22741 cagaatttga gccaatacat cgcctgcgtt gacaacgaca ctttcctgac ctgtcaaacg
    22801 tctaaagatg atacctgcga catgggcctt aatgatttgt gttttttgac gagcaattcg
    22861 gacatcgaca ttcactttgt caatattcgc ctgcgcaagg tcattttggt attttgcata
    22921 ttcaatttgt gccaattcat attgccttct cgagttaatt ccctgttcgt acaatttctt
    22981 ttgtctgtca acattggctt tacctgcagc aatagcttgt ttggctgctt ctatacgaag
    23041 taaaatcgcc tttttctcga cttcaagacg tgacaaaagc tccggatcat tatcggtaat
    23101 atcgactatt ggatcccctg gtttcacacg catgccttcg tcgacatacc atttctttat
    23161 tcgcccgcta acaggtgaat taaccgtatg ttgcctctct gttggtgaaa aggcgatgac
    23221 ttttccgttg cctaatgcaa actgttgcca cggagtaaaa cttaagaaaa gaataatggc
    23281 aataaaaaca atgaaaatta ttttcgctat tttttgaggc ttgggaagtt tactaatcat
    23341 agaataagca tgcaatttca tgaggaaatc accaggcgat tatttaaata gttaaaatcg
    23401 gagcgttggc tcacgatcgc taacagcgta ttttttaaag acaataccaa ttccatcatc
    23461 tcattcactt cttgatgatt gaggatgtca aaagcgcgat caataacgat taattcaggt
    23521 tgtaatagaa gagccctgac taacatcaat ttggtgagct caactaaggt aaacacatcc
    23581 tgccattcat agagaatagt gcgtagacct tcaggtagtt gtatgatttt atcagcaagt
    23641 ccaacattct ttaattgttc aataatgatt ttattaggca cgttgcgatg attgagtact
    23701 aagttgtcat aaatgcttcc ggtaaaccac tggggctctg caatcaacaa actaaaacga
    23761 cgaagagaaa taaggttttt tctcgcacaa ggaatctcat taataagaac atgaagcgag
    23821 gcaggatctt ggaatccaaa tagttgctca acaaacaatt ggcataaatc agatttttcc
    23881 gaaaacacca ataaaggctt atcaactgct atcaatgctt tattcttgct atccatctct
    23941 aactggatgc tattaattgg ggtcaataac tcgccgtgct catcttcttt cacctgttca
    24001 agtgacaggt taagtactgc atcgatttta ttttctgagg cgaccagatc ataataattt
    24061 tctaaaaggg caccaaaacg tctaaacgag taaatcagag cacctaaaac gatttctgcg
    24121 gcaaccaatt gtccaagact taactgatta ttaataacaa gatagccccc caatcctaat
    24181 aaaagactac tcgccaaagc agacagcgtg taaaaaccaa tttgatgttt gataagctgc
    24241 ttaaaatggg tatttcttgc ttttaagaaa gcaaccaatt ttttatccgt ttgttgtgtc
    24301 acatagcgat gatataaact aaaacggaac agataccggt taatcaaaat ttcttctaac
    24361 catgcaccta caacgtgttt ttcagaacat tcttttttgg cgctgtctaa tgcttttttg
    24421 taggggataa aaatgatcag cagaatgcca agaatgataa aaatatcaaa caaaaggaaa
    24481 agaggatgat aaattagaat cagcaatagt ccaaaaaaaa gttgtaaccc caaattaata
    24541 ccatacaaca ataaactcgc gagtgatttt ttgatagtga ctatttcaaa aaagcgattg
    24601 accaactccg gtccatgatg tgtcgaaaaa ttatcaaggg acatgtgggt gaaatgtcgt
    24661 gtcagactta aactaacttt aaccatcagt ttttgctgaa ttacttcaat aatgacaatt
    24721 tgccatatgt ggagagcgcc tagagccatc attaaaatca ataccatcaa acttaacgta
    24781 atcaccggtt gcaatagctt gccaaaagca attaaattca cgagtgtttg ggcagcaatg
    24841 gggatactca atgacagtaa gctgataatt acactaatca tcgcgatgga tgagattgtt
    24901 tggcgatcta atacatcaat aattgaatta attggttttg cattcattcc ctaaacatcc
    24961 cttttaccct tgattcttgt taatgctatt aatattttaa tttttgcaat ataaaaaatt
    25021 ccagcgattc aatcgcttta ttgcttccac tcttttttaa taaagaaaca tggacatcat
    25081 tcaatatagg gagaaaatcg taatcgagcc gttccagata gtttgggatc atgctgcgct
    25141 gaatagcagt aatccctaac ccagcgcgca cagctgccat ttttcccgca taacttgcgc
    25201 tactgtaaac aaggcgccaa tcaatatggt ttttatccag tgtatcaatc acattgcctc
    25261 gataaacgca gggtgttggt gaaagcacta agggaattgg ccccggtttg tctaaaagag
    25321 gtaataattc ttttttccct atccactcaa cgggttctgt acaaacatag atagactcag
    25381 aaccaacctg ttgctgagtc gttttaatga gaattaagtc aaatttacct tcattaaaac
    25441 gctcgatcag attcagagtc agatcacatt caacattcaa taagactcgt ggatgcaatc
    25501 ttgaaaattc aacgagaaca tcggataaaa taatactggc aaaatcctct ggtaatccga
    25561 agcggatttc accttgtaac tcaggcgctt taaaacgatc caaagattct cgatgcaact
    25621 cataaattcg tttggcatac cctaaaaata tttcaccatc cgtagtcaga gaaagttccc
    25681 ggccacgatt aattagaggt ttttcaatca ggttttccaa tttagctatt tgctgactga
    25741 tagccgattg ggtgcgtcca acccgttgtg ccgccttggt aaaactttgc gtttcagcca
    25801 cggcgagaaa gcattgtagt gtgattgtat caattgacat aagtttttct aatagtttaa
    25861 ataattattt ttaattttac ttatattaac atatctaata tatttccaca ataataacag
    25921 aatttctaat ggagaattaa tgtggataaa tttttaaatg ggttatcgat ttcactcatg
    25981 ctatcgccct tgatagcgat gctattctat atcttgttag gaaaaacaag aaatattcaa
    26041 gcaacttatt acggtaccac agttataggt tttggttttg gattgagtct cttggcacta
    26101 attgtttctg caaatactgt aaaccaaaac agttattttt tatttcatgc cagcacacta
    26161 agcctcttgc taagcacatt aatattattc atcagcttta ttatccaccg tttttcccta
    26221 cgatatatgc atggtgacaa gtgctaccga cgttattttt tcaaactttc agccataact
    26281 ttaacaactg ttgtgatggc tttagcagac aatcttttat tgttctggct tgcctggtca
    26341 gctagtaact tgctcttgat aaacctcatg attcataagt cacaatggaa agcagcctac
    26401 aactcaggga tactgacttt aaaaacacta ttaccaggaa gcttgttgct tatcacggca
    26461 ttttggattc tttttacgtt aaacaatact ctgtcaattg aaacaataac cagcgaagtg
    26521 agcaataagt catcccttgc tattgccgtg gcacttgggt ttattatctt agcagcgatg
    26581 gcacaatcag ccatatggcc ttttcatcgc tggctaatga gctcattaaa ctctccaaca
    26641 ccggtatccg ctctaatgca tgctggatta gttaatggag ggggctttct gattgttaaa
    26701 tttgctccgc tgttcgtaac gcaagaaaat ttattgacca ttttattcat tttcggcgca
    26761 ctgactgctt tattaggcac cctctggaaa ctgttgcagt ggaatatcaa aagtatgctt
    26821 gccagttcca ccatggcaca aatgggtttt atgatgatgc aatgtggctt aggacttttc
    26881 cctgccgcta tcgcccatct atgctggcac ggcttgttta aatcctatct ttttctcaat
    26941 tcaggctctg ccgtccaaca aaagaaatat caaaataaat cacgtcctga aaaattgaca
    27001 aacttattga ctgcattttt tggaggactt agtggaatgt atggctttgc ccttatcaca
    27061 gggaaaccca tcttatctct gcatgcctcc acctttcttt taggttttgc attcatcaca
    27121 ggaacacaac tcaccctggg cttgattgca tatgagaagc atttcaaaaa agtaatttta
    27181 gccttggctt tagttttctc aattggaatt ttttatgggg gaagtattca tctcatcgag
    27241 tcactcttac ctaatctaac aggatttcct caataccaat taagcagctt acaagtctta
    27301 actctgatta tcttcttttc cctttggctt ttattcagca tggggttatt caaaaaaatc
    27361 agagagacca gactttggga ttggttttac atgtacatgt ttaacgcaag tacgcctcac
    27421 cccaaaacta ttacggcaaa tcgccacact tattactatt aagaaaagga gtcaacatga
    27481 gtagccacat cacactaatc aaaactaaca caagcaatga aaatcaaatg acccaatgca
    27541 actacccagt cgataatgat gtcatgatta tccaggccat ggtaaataat gttacaaaac
    27601 aaattacacc tgtatggcct ctggaaaaat ttattgcttg caattccttg catggttttg
    27661 aatcgatgtc ctttgaagag gctgtgatac aaaaccagac ggctaaaaaa ggcactccat
    27721 tcaatgaaaa actagaaaga gtcaattggc acatgatcaa atggtgtggg agctttcttg
    27781 acattggaca aggcacgatt gaaatgcctc atcgtgataa aggcttatat tttggttttt
    27841 taaagctagc cccttttgac agcacactgc atcaaaacaa taaatcaaca aagaactggt
    27901 tatcaaatct gccagaaatg cctgaacaag ctattcgact ttgcctcgat aaacttggcg
    27961 tattaaacca aagacaagag gaatttctcc aatccactct gtctcactta cctggatggg
    28021 ctggatacat taagtggata agtgaatgga aaaatcgtaa tggcaaggaa gaaaatcctg
    28081 taagcctagt cgattttctt gcagtgaggc tgataatcac ctgcatatta tggccagaag
    28141 caaatctgga agaaaaaaaa aaggaaaaag atagtgcgga tactaaacaa cttattcaaa
    28201 atataaaaca caaagaagat gattacaggc aattgctttt aaaaaagctt ttgcctgagt
    28261 taaacaaagt tcagataaaa ggaaacagag ctaaagccca aatggttttt tgcattgatg
    28321 tccgctcaga accatttcgg cgatgtatag aaaaactagg acactacgag actttgggat
    28381 ttgcaggatt ctttggttta cctgtgagta tcaaagatta tgacggtgaa acaataaaag
    28441 actcctgccc agtactatta aagccacgat ttaatatcca tgaaaaagca attgctgcaa
    28501 atgaacattg cctagagcat catgagaaag gtaaagagtt taaaaaaatt ttgaaccggg
    28561 tctatcagca attaaagtac aatttttcaa ccccatttgc cttagtagaa tcattaggaa
    28621 tctggtgtgg gattacaatg ttcctcaaat catgtagccc aattttcgct agacgcctta
    28681 cccaagacct aaacgaaatg atatgcccct cgattcaaac acagcctgtt tttgaattgg
    28741 atttattaga gaaagaggta gggatctcac ttcaagaaca aattgcctat gccgaaatgg
    28801 cattgagact catgggactg actgataact ttgccaaact tgttattttc tgtggtcatg
    28861 gcagctctac tcaaaataat ccttatgctt cagccctgga ttgcggcgct tgtggaggta
    28921 atcaaggtgg aaaaaatgca caattactgg cgtctatcct caataaaata acagtgagaa
    28981 gggctctggc agaaaatgga atcaatattc ctcaagacac tgtgttctgt ggcgcgcaac
    29041 atgacacgac gacggatgag gttgaaattt atcattcaaa tgtcagtcaa ttcattgacc
    29101 aagatatcct cgaccagctt agagctgact taaacatggc taagcacaat aataatttag
    29161 aacgaattaa ctatttgaat tccatagatt gtgcggagaa agacattgta cggagaagcg
    29221 cagattggtc tgaaacaagg ccagaatggg gacttgctcg caatgccgct ttcatcgtag
    29281 cacctcgcca actgaccaaa aacattgatc tggaagggcg ttgtttcttg cattcttatg
    29341 actggagcaa ggacgaagac ggtaccctgc ttgaaaccat tttgactgcg cctatggtgg
    29401 ttgcccaatg gattaatact caatacctgt tttcaacaat cgacaacgtg gcatatggaa
    29461 gtggcagtaa aatcactcat aatgtcgcag gaaaaattgg agtcatgcaa ggcaatgcca
    29521 gtgatttaat gcatggattg ccactgcaat ctgtcatgag ccatgatgag aaatcctttc
    29581 atgagccaca gcgcctgtta actgtagtct atgctcctcg cgaaatcatc agtgaactag
    29641 ttgaaaaaca cgatgtctta aaaaccttgt tttttaatga atgggttcat ttggtagcga
    29701 ttgatccacg gagtcacttg ttttacaagt tagagaaaac caatacatgg tctgtaatta
    29761 agtgaaagga gtttcctgat gaatataatc aaagtatcag aaaaaaaaga gatgaacatc
    29821 tctttgcatc cgatgaaaaa gatcgaagtg atcgtatctg gtgacaatga aaatatggtc
    29881 gctaccatga tggagaaggc aaatatttct ggttttacct tattgcgaaa cgtctctgga
    29941 aaaggacatc atgggtttca tgaaggtaaa attttgttta acgacaaggc ctctttcgtc
    30001 atgtttattg cagtggcgcc tgaggaagct attgccaaaa ttgcacttgg catgaagact
    30061 ctattccaaa agaattcagg agtcctcttt gtgtcggatg tttctgttgc tagagtcgat
    30121 tatttctctt agtatttaac tttggaggtg acccctatgc actatcaaaa tcagcgtgtt
    30181 ttactggcct caaaacacca aaaggaaaaa gcgattcagc ctccttttga ggagaaatta
    30241 ggctgtttta tccatgtccc tgctgattat gatactgatc agttcggcac cttcaccggt
    30301 gaaataccaa gaaaagcctc agcctatgaa acattaattc tgaaagctaa agcagcggcc
    30361 aagcaatttg gataccgtta tgttatatcc agtgaaggaa gcttcggccc ccatccccag
    30421 ctttattttt gtcccgcaga caccgaattg atggcctttg ttgacttgga taatgattta
    30481 attatcgcgg agcacgaagt atctacagag acaaattata gtcatattga tatcagtgcc
    30541 acagatgact atgaagcgtt tctcaacaaa gcaaaatttc caagccacgg cattattgtt
    30601 cgcgccttaa atagcgaaga aaattatatt caaaaaggaa tctgtgagcc tgagcaactc
    30661 aaaacaacga ttcaaaaagc gttcacatac agtacaacta tccgccttga aaccgatatg
    30721 agagcgatga tgaatccact tcggatgaat gtaattaaca aacttgcggt gaaactggtt
    30781 caccgcattc agcaacattg ccctcaatgc cacactccag ggtttggaaa aatctcggct
    30841 gaaggtcatt tagcctgcat ttcttgtgga acaccaacag aactttacct gcgaaaagtt
    30901 ttaaattgtc taaaatgtga atttaagact tatcaagcgc gagacgatgg actagaattt
    30961 gctgagcaaa ggtattgtcc ttattgtaat ccttaaccgt ttgcgagaat caagtccatg
    31021 gtatgttata aacattcaaa caagagaggc acattatgaa acccatttta ataggtctaa
    31081 ttatgggttc tcaaagtgat tggcaaacat taatccatgc cgcccacaca ctggatgcgc
    31141 taaacattgg ctatgaggca gaaattgtct ccgctcatag gacaccggac aaacttttcc
    31201 ggtatgccga acaggctgag gcaagaggtt tggaagtgat tattgctggt gcaggaggag
    31261 cggctcatct ccctggcatg gtagcagcca aaacatcttt gcctgtttta ggtgtcccag
    31321 tcatgtcaca aactttaaac ggcgtcgatt ccttgctatc cattgtgcaa atgcctgcgg
    31381 ggatacctgt aggcacttta tcgataggta aagcaggtgc catcaactcc gcactgtttg
    31441 cagccgcaat tcttgctaat aaatatcctg atattcgagc tgccttaaaa cactatagag
    31501 aacaacaaac acaaaaagtg ctcgacaatc caaaccctaa agaggaaaag tcatgaaact
    31561 tggtattcta ggtggtggcc aacttgcaag aatgttggca ttagcagcat atcccttagg
    31621 gattaacact gtctgcctgg atcccagccc tgatgcttgt gctggccaac tcaccaggct
    31681 gatagtctct gattttaatg atgaattggc tttaaaacaa tttttgtcag aagtggattg
    31741 cgtcacaata gaaaccgaaa atataccatt ttcttgtgcc gaattaattg cccaaataaa
    31801 gcctttttat ccatcaaccc aagctttgaa aattacgcaa gacagattat atgaaaaaaa
    31861 ttttttcagg tctctgaata ttcctactcc agaattttta ccagttgaat ctgaaggtga
    31921 attgatgcag gctattacgg acataggaat gcctgccgtg ttaaaaacaa gaaggtttgg
    31981 ctatgatggc aagggccaat ttgttttgcg agcccaatca gatatttcaa aaagttgggg
    32041 cctgttaggc agtcagcctc tcattctcga agagttaatt tcctttgaaa gtgaattttc
    32101 gctaattgct gttcgcaata agagtggtga aacaagtttc tacccattga ttaaaaatca
    32161 tcacaggaag ggcattttac atttctcaga agcccctgtt catcaggaca ccttacaaca
    32221 ggaggcgcaa actcatgcat tcaaaatttt agaagccctg aattatgttg gtgtacttac
    32281 cattgagttt ttttatcaag gcaaccaatt gattgcgaat gagatagcac cacgcgttca
    32341 taattctggc cactggacta tcgagggagc acatactagc caattcgaaa atcatctgcg
    32401 cgctcttttt gatttgccct tgggaagcac tgaagtaagc ggctcttgtt ttttattaaa
    32461 ttgcattggc aatctgctgc cattggagcc ctgtttatcg atcccgggtg ttcattatca
    32521 cgcttatgga aaagcccccc ggccagatcg caaggtaggt catgttaccc ttatcgatac
    32581 tcatttggaa cgttatgcaa aaagcaaaga cttacttctt gacatttcat gagtagcaga
    32641 tagttagaaa gcgagcaata ggagtcagcg cataatattg ttcgcaattt ggaaagctga
    32701 gcgttggatt ctgttgccta ctgctcatcc acattatcct gcttattatt ggttgtcact
    32761 atagactcag gatcaatgga gcttccggga atttttcggc tttcgctggc ccattctccc
    32821 agatctatta acttgcatcg ttcagagcaa aaaggcctga acaggttatc agggcgccag
    32881 gtattttgtt tgtcacaaat ggggcattta attttttgct ggttgttcat cacggttttg
    32941 ctaattcata ttgtttagat atgaagtatg gctacaccag caatgatttt caatatatct
    33001 gccttatgat tgatatgaaa atagagctct tgcagccccc aatctaaaac taattatcaa
    33061 catgcacttc acgcaccaga gccaaccaag cttgggccgc ctcagagaga taagaatttc
    33121 tacgccaggt catcgccata ttccattgga actcacttcc attaagtaaa agagagcgaa
    33181 tttgtgtcgt atgcctttga gtggcaatca tccgtggcaa aaaagtaacc ccaaggcctg
    33241 ctgcaacaag ctccagcata aaatcaatct gactgctttg agccaccacc ttaggttcga
    33301 aaccagcgtg acggcacgcg tccaatataa ttcgatgtaa cgcgaaacca ctgccaaaaa
    33361 ggatgaaagg tatttcttta agttctggta gagaaatgca atgtttagtt gctaatggat
    33421 gtccagatgc caggagcgcg accaatggtt cagagcgtac taattggcaa tcaaattctt
    33481 cggaaatggg taatagggtc ccagcaaaat cgatctctcc tgcacgtaaa cactctgcaa
    33541 gtttatcgct gccatgttca acaagctcta cttcaatgcc tggataacgt tgtctgtata
    33601 gaacaaaaag aggggcaaat aaagtactgc tacccaccgg agaaataccc aaacgtagtc
    33661 gaccttgttt caaacctcgg atttcatcca attctttaag aagatcatct cgatcagcca
    33721 ataattttgt tcctcgttga taaacaattt cacctgcggt tgttggtaca ttacgctttt
    33781 taaaccgctc aatcagagtg atacctaact cctcttcaag ctgttttatg gctttactga
    33841 ctgtagactg ggtcgtaaag agattttcag ccgctttgga aaaactaccc tgccgtacaa
    33901 cctctatgaa agtctgaagc agtttaaatt ccataattat tccatattgg aaaggttatt
    33961 agtctataaa ttcattttat tcatcttaat taaaagtgta aacttaaatg aaaaaataat
    34021 atccaccatg agcacatata tggcaaataa aattcagcaa aattcatttt ttcagatagt
    34081 attggttggc ttattttggt tagctagtga gttattgctc aaactggtca agcttccttt
    34141 gtccggagga atccttggct tgggaattgt tttactgctt ttggcgacga attgcttaaa
    34201 attaaatcgt ataaggcggg gagctgaatt actccttgcc aatatgttac ttttttttat
    34261 ccctggcgtc cttgcaatac tggagcaccg ggaatttatc ggtcttttag gccttaaaat
    34321 tctcttcatt attctgctca gcacaatcac ggtgatgcta gtcactgccc ttgttgtaga
    34381 ttattgctac cgttggagga cgcatcatgc caagcagcat tctctctgaa tccatgatcc
    34441 aggcattttt ctggtcactt atcacaatag gtatgtattt tattacgcaa aaactatata
    34501 gaagatggcc cttttggtgg ctaatgccac ttgctttaac accgatactg gttgccagcg
    34561 tagtgctttt actgcatgta aactaccagg actatttccg aggaacaaaa tggctagtct
    34621 tgttaattgg tcctgcaacc gtggcctttg ccattcccat ttatgaaaaa cgtgccctca
    34681 ttcgccagca ttggccagtg cttttaattg gcgtgattgt gggaagtttc actgccatag
    34741 catccagttg gggattggca atccttcttg gccttgatga taatttacgc ttaagtcttc
    34801 tccctcgctc tatcagcaca ccgtttgcta tagaaatctc aaggggaatt ggtgcagcac
    34861 ctgatttgac tgcgattttt gttattgtca cagggatatt gggagctata ctcggtgaat
    34921 tcatcctgtc gcgcttgcct tttaactcag ctcttgccag aggtgccctt ttaggagtag
    34981 gagcccatgc ggcaggaacc gctcaggccc ataaaatagg gaatacggaa ggcgccattg
    35041 ccagtttggc tatgatttta gtaggtctat tcaatatact acttctccca cttatcagtt
    35101 atcttttgaa ataaaagtgc gtttagtaga aattaaaagg aactcctcta gttaagaaaa
    35161 aatcataagc atgttatttt atggtttttc catcaataga agaaaataat gactattgct
    35221 cttgtgtggt tccgtcaaga tttaagattg accgataatc ccgcgttcat cgaagcatgc
    35281 tcccatcatg aaattgtaat tccactttat atttatgatg ataaatgcag tgttttaggg
    35341 caagctcaag cttggtggct ccattatagt ctcattgctt tgggcaaatc gttaaatcaa
    35401 cagggtttaa gcctcgttct ccgcaaagga tctccgcaag agattatttt ggaattagtc
    35461 gcacagtatg gtgtggaatc agtctattgg aatcgctgct atgaaccaca ggtcattgca
    35521 cgcgataaaa aaattaagct tgctttggtt gaaagagaaa ttaaagtgca gagtttcaat
    35581 ggaaatttac ttcatgaacc gtggacaatt aaaaataaaa atggggattt ttttaaggtt
    35641 tttactcctt actggaaaca atgtcgacaa attcttaata tcccctcgcc ttttattctc
    35701 actaaacgcc caaggggaat cgaaattgca agtgatgaca ttggcaaatg gaagttattg
    35761 ccagcaatta attgggcagc acgattttct gaatattgga ttcctggaga agagggcgca
    35821 caaaaaaaac tggatgagtt tattgttcat caccttagcg gatataaaag ggatcgcgat
    35881 tttccagcta aaaatgcgac atctcgattg tcacctcatt tgcactttgg ggaaatcagt
    35941 ccttgggtga ttttaagagc actcgagttg gctaaactgg aacatacctg tgatctggcc
    36001 tccgtagagc attttttatc agagctgggg tggagagagt tttctgtcta tctgctctac
    36061 cacttcccca agctggactg cgaaaatttt agaaaggagt ttgatgcatt cccctggcaa
    36121 aatgatgagc aattattaac ttgctggcaa aagggaatga ctgggtatcc tatcattgat
    36181 gctggaatga gagaattatg ggccacagga tacatgcaca accgcgttcg catgattgtc
    36241 gcatcatttc ttaccaaaga tttactgatt gattggcgtt cgggcgcaca gtggttttta
    36301 gacaccctag tcgatgcgga tctggcgaat aacagcgcga gctggcaatg ggtagcagga
    36361 tgcggtgcgg atgcagcacc ttatttccgt attttcaatc ctattttaca aagccaaaaa
    36421 tttgacccgg atggcgttta catacgccaa tggatccccg aacttgccca attaaatagt
    36481 cagtcaattc atcaaccctg gacagatgct gacgccagtt ccattttttt agggaaaaaa
    36541 tatcccaaac ccatcattga tcaccaagaa gcgagaacta aggcattgag ttactatcag
    36601 caaataaaaa aaggaatgga ttgacaagag attaactaaa atttggagag accccattga
    36661 ataaaaaaag ttggacagag ctgattgcct ggatcattac ctttgaagca attggttttt
    36721 ttttaggaat gctgacgcaa gcgaatattc cctcttggta tgaggggctc aataaatccg
    36781 gtttaactcc tcctggacca gtattttcaa ttgtttggtc gattttatat gcccttcttg
    36841 caatagtggg ttgggtttta tggtgctctc gaaatgagtc tgagatgaga tctgtgtttt
    36901 atttatattc agtacagatg ctgatgaatt gggcatggac cccgcttttt tttgtgctgc
    36961 attggactgg tcttagtttt ttatggattc ttgtaatggc ggggctcact gggcttctga
    37021 tgtttagcca aacaaataag aaaaaaagcc ttgctatatt gttgctaccc tatctcattt
    37081 ggttgctttt tgccgcctat ctcaatggcg tgatatggct tctcaattaa ctgttcccaa
    37141 gttttccctt aagccaaata caaacttggc tatagcgaaa tcgtgctaaa tcttactttg
    37201 cgagataag
//
LOCUS       DACTJE010000013        37002 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.013, whole genome shotgun sequence.
ACCESSION   DACTJE010000013 DACTJE010000000
VERSION     DACTJE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 37002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37002
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.013"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1608
                     /gene="sidG"
                     /locus_tag="I9760_000459"
     CDS             <1..1608
                     /gene="sidG"
                     /locus_tag="I9760_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidG"
                     /protein_id="HAT5042254.1"
                     /translation="ALWEEGIDVEEVNSAKKDISKPAPEIQKLTDLQEQLRNHKESGV
                     KLSSELEETLNSSVKMWKTKIDSPCQVISESSVKALVSKINSTRPELVKEKEQLPEQE
                     ESLSKEAKKAQEELIKIQEFSQFYSENSSAYMVIGLPPHHQVSLPLAVNGKRGLHPEA
                     MLKKMHELVAGPEKKEFNLHTNNCSLTSIEVLSAGAQHDPLLHSIMGTRALGFFGTPQ
                     QVLENAKLTSKTINEGKKSNIFTPLVTASPLDRALGYAMSIYMDPEASKAKQNAGLAL
                     GVLVGLAKTPGIIIGSLLNPKQGFNDILNTLNLVYSRNSTGLKVGLTLMALPAMIVLA
                     PLAAIQKGVEVIAETIAKPFKLIANLFKQKPESTDEITVSVGSKKVAEKEGSYSNTAL
                     AGLVNSKIKSKIDENTITVEFQKSPQKMIEEFESQLKENPGKVVVLSEKAHNAVLKFV
                     SKSDDEALKQKFYDCCNQSVARSQKFAPKTRDEIDELVEEVTSTDKTELTTSPRQEPS
                     MSSTIDEEENIDSEHQIETGTESTMRI"
     gene            1761..2936
                     /locus_tag="I9760_000460"
     CDS             1761..2936
                     /locus_tag="I9760_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAT5042255.1"
                     /translation="MPKLILLGDSTLDNFNWVPNKATVTDHLRRQLPKMEILNFAVDG
                     FTTDSILYGEYKDCAVTSSQHTHKYFEPLKALENEQDVEHIVLSVLGNDFRIQLSKLI
                     FMAPEERPKAIEQLINHIIKNYIQVVKEIRKVQPNAKLSIVLQYTPYVENDPYLIYFL
                     MDKLFHHGTVSHGFLSYLQIMYYSIFGLNSKHEQQALNLLHKLMAKVYRNVFNELGDN
                     SLAIIDLASSFDYRNSKLYKAQIEPSDEGGKLIASLITHTITHHDFQNESMLYAKPPL
                     QPNPKILAVPLTKLKKDWFPQQIYVTQEQAITAFKQAYLLQLNKDRQSYLGLYSFFAR
                     SNVDFEHATLEKLIVHAQNYSTKKTGYRTLKVMQNLGWLTEQGNIKEESGIVRLLKN"
     gene            complement(3037..4221)
                     /locus_tag="I9760_000461"
     CDS             complement(3037..4221)
                     /locus_tag="I9760_000461"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HAT5042256.1"
                     /translation="MTDVYVVDVLRTPVGKAPRGVFKNTLPDDLLAHTIKCLIHRHQN
                     IDWQEIADVVIGCAMPEAEQGMNVARIASLLADLPQSVPAMTINRFCSSGIQSIVTAA
                     DTIRNQEAHLALGGGVESMSMVPMGGNKYTANPSIFNDEHVAIAYGMGITAENVAKRW
                     EITREQQDEFAIESHKKAIAAQERLDFNAEISPIEISIRYPDLKTSKVEIKKKLINKD
                     EGPRADTSYEAISKLKPVFALNGTVTAGNSSQTSDGAGVALLASEKALKQYDLKPLGR
                     LMSYAVAGVPPEIMGIGPVKAIPMALKRAGITLDQLDWIELNEAFAAQALAVIKDLDL
                     PREKVNPLGGAIALGHPLGATGTIRTATLLHGLRRTKGRYGMVTMCIGTGMGAAAIFE
                     SL"
     gene            complement(4237..6606)
                     /locus_tag="I9760_000462"
     CDS             complement(4237..6606)
                     /locus_tag="I9760_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HAT5042257.1"
                     /translation="MQEPFFIKKVAVLGAGVMGAQIAAHCVNAGIETLLFDLAGKEGP
                     KNSLVDKAIANLGKLKPTPLATPQTNELLQAKNYAENLEELSSCDLVIEAIAERVDWK
                     EDLYKRISPYLSDKTILVSNTSGLSINKLCEVLPAQHRSNFCGVHFFNPPRYMHLAEL
                     IPANTTSSDLLDYLETWLTEYLGKGVVRAKDTPNFIANRIGVFSLLTTLHHTLAMNMG
                     IDEVDALTGPLLGRPKSATFRTMDVVGLDTMQHVINTMQSQLTGDPWHKMFILPDWLN
                     GLIKEGHLGQKSGQGIYRKAGKVIEVYDIQTGQYRPSRAEVSDELKAIMDIKDPEARM
                     KNLMASNNKQAQFLSACFRDLFHYCAYHLEEIADNARDVDLAIRWGFGWSQGPFETWQ
                     LSDLHSVTRQIDESIQDNTALSSAKLPEWLYEIKEFYTREGAYSPGKNNYHARSQLPV
                     YHKQFFHDRVLKESHHPVHVIYENEGVCLWYLKDDVAVISFKSKANTIGQSVLDGIET
                     ALDIAERDYQGLIIYQQDASNFSSGADLRGVSKLLTDGTIQALEHMVEQFQRVAMRLK
                     YSSIPTVAALRGRALGGGCELMMHCDSIVAAFESYPGLVEAGVGVIPAGGGCKELAMR
                     SAHHAQQADLLTFILPVFQQIATAKVAASAVEARNMGYLRAADSILMHADEVLYASLE
                     KIKAMRAANYLPPMKKQRFKVAGIEGHARLQSGLVNWLEGGFISQHDYFLANELAKVL
                     CGGDVNHGTLVDEQWMLKLEREAFIKLANTSLTQARISHLLETGKPLRN"
     gene            complement(6726..8246)
                     /locus_tag="I9760_000463"
     CDS             complement(6726..8246)
                     /locus_tag="I9760_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAT5042258.1"
                     /translation="MDLLQRLNIKSVNPGAFSGHGWHSVNHAHTLESFNPSTGNKIAE
                     IATCTMDDYEQVMQRAEQAAQAWRKVPAPKRGEIIRQIGQVLRENKDSLGSLVSLEMG
                     KSKQEGDGEVQEMIDIADFAVGQSRMLYGNSMHSERPNHRMYEQWHPYGIVGVISAFN
                     FPVAVWSWNAFLSAICGNVTIWKPSAKTPLCAVAVQHICNQVLKENNCPEIFGLIIPN
                     SHDVVEAMVDDKRIQLISFTGSTAVGKQVAAKVAARLGKSILELGGNNGIILDESADL
                     NLAIPAIVFGAVGTAGQRCTTTRRLFVHESKYQDVIKRLRHAYEQITIGDPLDTRNLM
                     GPLIDLQAVEQFKKAINRIKAAGGQIVYGGEILKQAGSFVQPTLVCDVKNDWDIVQEE
                     TFAPILYVMSYRTLDEAIALHNGVPQGLSSALFTQNLKNAERFLSACGSDCGIANINI
                     GTSGAEIGGAFGGEKETGGGRESGSDSWKAYMRRQTNTINWGDELPLAQGIRFNLS"
     gene            complement(8256..9344)
                     /locus_tag="I9760_000464"
     CDS             complement(8256..9344)
                     /locus_tag="I9760_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HAT5042259.1"
                     /translation="MYNVMITGAGKIGSLIACLLADSGSYQVHLADLEFNGSDVTRLL
                     TALPEIKTVALDVKDEQSTQAYLQKHNIIAVISSLPYFLNTHVAKAAKAAKAHYFDLT
                     EDTSVTEAVKAIAQNAETAFVPQCGLAPGFISIAANSLMQEFEKCHHARLRVGALPQR
                     ANNALHYSLTWSTDGVINEYGNPCYGIEGGKDVVMAPLEGLESIQIDGCEYEAFNTSG
                     GLGSLAELYAGKIQSMNYKTMRYPGHCKKMRFLMNDLRLNEDRGTLKRILENAIPKTY
                     QDIVIVYVTVEGIKQGELTEKSYVKKIYPEVIRGLEWSAIQVSTASGVCAVVDLVLGQ
                     GNEYKGLILQEKFKLSHVLENRFGKYYA"
     gene            9499..11034
                     /gene="lnt"
                     /locus_tag="I9760_000465"
     CDS             9499..11034
                     /gene="lnt"
                     /locus_tag="I9760_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAT5042260.1"
                     /translation="MKQAALIHSTIINTSTLNQYSKFVVVFLAGLLAPLGFAPFHLPG
                     LTILSLAILFSEFLQCTVKQGFSLGFLYGLGYFGFGVSWVIISIHDYGQLDYFLAGVI
                     TLLFIMYLSLFPGIVGYSFKLLSTKCNLLTCMLLFSTLWCIAELIRSKLFTGFPWLLA
                     GITQIDTPLRYIAPVVGVYGLGLICSFLGTLLVLIIKDNTIKRYYYLSVFILILIAPS
                     LGKNIHWTEIKKEPISVGAIQPNLSMRDKWDETLFWDLLKYYENKTDKLLGKNLIVLP
                     ESAIPLPASYVEDYLQKLHEKALKAKSSLILGILQPTDNDETNFYNSIISLGEATGAY
                     VKRQLVPFGEYIPKPFIPINRWLNLPEPNVVPGRSNQPLINIANHPIASLICYEIAYP
                     NILRSQMPAAQWIVSISDNGWFGHSLASYQQLQMAQMLSLLTGRYQILVNNDGLSSVI
                     NHQGTIVEGLPAFNSGMLQTDIFPANGITPWIIWSDYPTFVFCFIFVLFVLFFNIKRF
                     TEK"
     gene            11100..13571
                     /locus_tag="I9760_000466"
     CDS             11100..13571
                     /locus_tag="I9760_000466"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAT5042261.1"
                     /translation="MDNTYNPQEVEEQAQQYWHKKQSFNVTEDLNKEKFYCLSMFPYP
                     SGTLHMGHVRNYTLGDVIARYQRALGKNVLQPIGWDAFGLPAENAAIKNKIPPAEWTR
                     KNIAAMKEQFLRLGNAYDWKREITTCDPEYYRWEQWFFIRLFEKGLVYKKNAVVNWDP
                     VDQTVLANEQVVDGRGWRSGALVERKEISQWFIKITSYADELLSSLDSLDEWPAQVKQ
                     MQRNWIGKSIGTEIYFNVNNYPKRLKIYTTRPDTLMGATYLAVATDHPLAKEAASNNK
                     KVQEFLDSCQGIKIAEAELATMEKRGIDTGMTAIHPITGKELPIWVANFVLMQYGSGA
                     VMAVPAHDQRDWEFAQKYQLPVKQVIKPIDIEHDFNQSAYTEEGILINSNQFDNLLSS
                     KAIQVITNFLEENDAGKATINYRLRDWGVSRQRYWGTPIPMIICEQCGIVPVPDEELP
                     VVLPENVDFTGTGSPLTQCKEFVNVTCPKCGQDATRETDTFDTFVESSWYYARFACKG
                     QENAMLDDRAKYWTPVDQYIGGIEHAVMHLLYARFFHKLMRDEGLVNSDEPFKALLTQ
                     GMVLKDGHKMSKSLGNVVDPNHLINTYGADTARLFVMFASPPEQSLEWSDSGVEGAHR
                     FLKRVWAFSHQHRDMLIDINDSILSGNGHVDWKEAESRLKKSRHIVHQILAQATHDYD
                     RNQFNTVVSGCMKLFNEISDYSIETENDKFFIHSSISILLRLLAPITPHICHCLWQQL
                     GFDKAIIDAPWPKVDKSALKTDEVDYVVQVNGKLRAQFTASTDATEEELIAAAKEHAH
                     NFVVNHTIKKAIIVPHRQLINLVIG"
     gene            13673..14164
                     /locus_tag="I9760_000467"
     CDS             13673..14164
                     /locus_tag="I9760_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042262.1"
                     /translation="MTQIHKYLASLILIWLLTGCGFHLRGAIDIPEWLDNVSIISRDG
                     NKELVSILKSQLEGYKIDVNSDPSLAKYWLIINSSSLQQQIVSIGASTNPRQYQLILT
                     IEFMLQTPKGQIIKTPRKVNVSRQLTVNNDRILGSNEEQALLVSEMRRDAAIQIINRL
                     NRP"
     gene            14166..15191
                     /gene="holA"
                     /locus_tag="I9760_000468"
     CDS             14166..15191
                     /gene="holA"
                     /locus_tag="I9760_000468"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAT5042263.1"
                     /translation="MQINQQALTQLLQKKIHHLNVLIGQDHYLIDEALNNIKSMLKKS
                     SECDEKILSIQSPEEWHVVLEETNNYSLFYQTVILTIFFDKKTIDTTGKKILSEYLKS
                     ANSHSYIIIRAPNLTAKQLQWLTHDEQALIIIAQPLSSEAMKRWINEQLKLNTISFDP
                     YVPELIYQYTKGNMLATAQVIEKITLSNTPGTLITPYLALEHLSDQCDFSLFELIDSC
                     LLGHSDRAIHILRQAANNKTEVTLVLWMLTQEIRTLLQLLFLMQQKMDIKMASNQLKI
                     WTQRVNLYQSAIKRLNPLLLQQLHHYCQSIDDKIKSNSNNQVWNALENISLSLCLGQL
                     LGDACIA"
     gene            15179..15814
                     /gene="nadD"
                     /locus_tag="I9760_000469"
     CDS             15179..15814
                     /gene="nadD"
                     /locus_tag="I9760_000469"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAT5042264.1"
                     /translation="MHSIAIFGGTFDPVHNGHIKTSLAIQANFGFDSYYFLPCKSPAI
                     KPPSFASSEQRVEMLKLALKPYPDFKIDTRELDRDTPSYMVYTLQSFRQEYTDSSLTL
                     IIGYDGLLNLPQWYQWEKIISLANLLVINREEFFQQPVPKSVQTLLNQYRNDDKNILL
                     NHHAGSICLYNAGHYDISSTKIREQLKQHKDVKNNLPDLVYDYIKKQELYQ"
     gene            complement(15789..16322)
                     /locus_tag="I9760_000470"
     CDS             complement(15789..16322)
                     /locus_tag="I9760_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAT5042265.1"
                     /translation="MQLQWVDIAIVAIIALSVLTGLFRGFVKELVALCVWVLAIWLGF
                     NYSQSLDPWLSSYIEEQSVRTAIGFIIILFATLFVGGVVNAILSFILKRAGLSGTDRT
                     LGMGFGFLRGVFIVALIMVAVKMTSLPYQEYSQKSKLYARFDPVVALLYSHLPEFIKQ
                     VKAVDKTENIIDTVPAS"
     gene            complement(16325..17095)
                     /locus_tag="I9760_000471"
     CDS             complement(16325..17095)
                     /locus_tag="I9760_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HAT5042266.1"
                     /translation="MKLIMDEKVKHRLIGLAVIISLGAIFAPALIKKSSQSPESNFSV
                     NVKLPPKPLPPDVVITDEKEVFKTIKVAKTEIPPIPVEKKSINLAKAEPISTEEDNLI
                     RTSKVATLEAERENSIKSEPIKLAVNKAANATAKKVMTLTAAKPPVKASPVVTAKVST
                     SKKVSQIVAARKPAVKQDIYAVQLASFTQIANAQALVNRLRSKGYKANYLKSNSRNGI
                     VYKVVVGHSPVKKDVIKLKTQLASAMQLNGFVVNTGVS"
     gene            complement(17092..18378)
                     /gene="folC"
                     /locus_tag="I9760_000472"
     CDS             complement(17092..18378)
                     /gene="folC"
                     /locus_tag="I9760_000472"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAT5042267.1"
                     /translation="MHAPWSEWDLNHWLTHLETRNTQEIQLGLTRVLTVAQKLNLQRP
                     NCKVITVAGTNGKGSTVTALETIYHQSGFRVGTYTSPHLLVFNERIRINLTPIQDKDL
                     CKAFSVIEEARYPTNLTYFEMATLAALWYFKQHDLDLIILEVGLGGRLDATNIVDTDL
                     AIITTIDFDHQSFLGDTLELIGYEKAGILRKNKPFIYADLSPPKSIVEFAVQLGASSY
                     LYGKDYFYQEEADHWGVTCQGKLFEQLTRPKIQLRSAAAAVMACYLMQNDLPVSYKNL
                     KKAMELIYIPGRLQLQKGKTDILYDVSHNLQSVRLLADTVKKINVKGQIHAVFSALKD
                     KDLYGLIMPLRDCVNRWYPAQLHNKRAASQEELLSVFREAEIFLDVCYNDPFTAFEMA
                     LKQASDDDLIIVYGSFFTVSHVMAAQHNLLEQKENQ"
     gene            complement(18359..19243)
                     /locus_tag="I9760_000473"
     CDS             complement(18359..19243)
                     /locus_tag="I9760_000473"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAT5042268.1"
                     /translation="MSWLKKLLPSRIRTETSQKKGVPEGLWVKCSGCNEVLYSTELAK
                     NFMVCPSCNFHHRISARLRIAQFLDESGQEEVAAGLEPQDRLKFRDSKKYKDRISQAQ
                     KATGEKEALIVVKGFLMQQPVVVSAFEFNFMGGSMGATVGEKFVRAVNVAATEKRPYI
                     CFTASGGARMQEGLFSLMQMAKTSAALAKFAETGLPFIVVLTDPTMGGVSASFGSLGD
                     IIIAEPNALIGFAGPRVIEQTVRQTLPEGFQRSEFLLEHGHIDMIVERKHLRATISEL
                     VSKLTHKGAESFFARAME"
     gene            19655..21082
                     /locus_tag="I9760_000474"
     CDS             19655..21082
                     /locus_tag="I9760_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947070.1"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HAT5042269.1"
                     /translation="MAQVINTNVASLTAQRNLGVSGNMMQTSIQRLSSGLRINSAKDD
                     AAGLAISQRMTAQIRGMNQAVRNANDGISLAQVAEGAMQETTNILQRMRELSVQAANS
                     TNNSSDRSSIQSEISQLKSELERIAQNTEFNGQRILDGSFSGASFQVGANSNQTINFS
                     IGSTKASSLGGIATATGTEVAGAAAADITIAIGGGAATSINSSANFTGALNGQDATSA
                     YAKAAAINDAGIGGLSVTASTSGTQAVGAIGGTAGDTYNLTINGVAIYTNLNVATALT
                     NSDLRDAINGVSNQTGVVASLNGGNMTLTAADGRNITVTESGTGFTAGTDGLTVTGGA
                     FDGALRGTLSISAVDTIAIGGTVANIGLSANISKDTVGIDSLDVSTASGAQTAIKRID
                     AALNSVNSNRANMGALQNRFESTIANLQNVSDNLSAARSRIQDADYAAEMASLTKNQI
                     LQQAGTAMLAQANSLPQSVLSLLGR"
     gene            21165..21446
                     /locus_tag="I9760_000475"
     CDS             21165..21446
                     /locus_tag="I9760_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042270.1"
                     /translation="MSIEPVSSVNKAAQTDQIKIVEEVSKPEIKNMVYDSALDAETKQ
                     ALDKATGLLQTIITEKISDKVLRKMPSDEYLQLLSLLDDIISGSIDKHV"
     gene            21509..23134
                     /gene="fliD"
                     /locus_tag="I9760_000476"
     CDS             21509..23134
                     /gene="fliD"
                     /locus_tag="I9760_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAT5042271.1"
                     /translation="MELSSPGVGSGLDVKNLVDAIVTAEITPSQVRHDKQLNSVNTEL
                     SALGQLKSYLTNLQTSLAKLSSLSQFYTMKSTISDPNYFSATLGTGATKNTYQIEIQK
                     LAQQHSLASSYMTNTGSGTITIDFGTYNSDKTTFTPNPSATSVNITITPGNDSLVAVR
                     DAINNTNSGITASIVQDSQGSRLTITSSKTGENYAMKISGGVASLNYDPTMGVNSLTE
                     TVAAQNSTVKINGLTLVQSSNQLNEAITGVTIDLKKAEIGKIVTLTVDDNKDQLTGLI
                     NEFIKQYNDTMTFLTNLTGYNNETKQGGVFQGDPQFRNLKLNLNKWATTPLANQEGPI
                     RSLADLGIITNKQGLLEIKQDKFKTALDNHYKEIGALFAKTAKANDTGINVKSINSTV
                     KSGTYDVVLTEFTPGVSMSGTIGGLSAISSDGITLTGSADLSGLSINVFSGSAGSRGQ
                     ITVTDGIAVQLSSLLDAYIEIKGDLEQRKDQLNNQVKQLNKIQNEIDIRRKSIETRYL
                     KQFTALDTLLSQLQSTSVFLSQQLANLPQLKLK"
     gene            23150..23554
                     /gene="fliS"
                     /locus_tag="I9760_000477"
     CDS             23150..23554
                     /gene="fliS"
                     /locus_tag="I9760_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAT5042272.1"
                     /translation="MKNLYSQISSQYKAIELQTRIETASPHELIDLLLQGARSHIATA
                     QGNIQRNQIKEKGEHIGKAISIIEGLKMSLNHDKGGEIAENLLQLYDYVQQILLKANL
                     KNDEDLLAQSNMLLAEVHQAWQAINSNAGNEL"
     gene            23877..24623
                     /locus_tag="I9760_000478"
     CDS             23877..24623
                     /locus_tag="I9760_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAT5042273.1"
                     /translation="MNTFKSSLAFVICLLLGMQSAQAQMRYGETLCNLPEYFCMKIKS
                     GDTWDRLFPNIEEQDIVRRINRMNIRLKPGMIIAVPKNIERLTIYDVSPFPRYIEPSG
                     EKTIYVSQKKLAWGAYDEDGELLWWGPISSGVGKCKVIGGCSTPTGSFRIIRKQDIDC
                     VSTVFPRRANGEHGGALMPYCMHFFRGYALHGSYEVPGYRASHGCVRMFIEDARWLNE
                     EFIDLPGGGMRGTRVILDAPDAQENPDNLQ"
     gene            24863..26161
                     /locus_tag="I9760_000479"
     CDS             24863..26161
                     /locus_tag="I9760_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042274.1"
                     /translation="MAHYNVQSESLNKASVSLIMPWMVWGLGCLFYFYECLLQVSPSV
                     MSNELMRDFSVTSHTLGILSGIYFYSYAAMQLPGGVLMDYFGPQRLLTLATVVCAVST
                     IAFGMTDNFFMACIARLMIGFGSAFAAVGTMKLAANWFPAQKFALLTGLMVTIGMMGA
                     IGGETPLALLIDNFGWRESMIIMGSVGLLLATLLILIARDTPKNFSPAIHQHPVEEER
                     LIPSLFTLIKNKQLWLVATYGGLMYMATPVFCGLWGVPFLMNKMLIAKSTAANFISLV
                     FVGWAIASPLWGLFSNRIGLRKPPMYIGCVGAMICSLLFIFAPITSSITMEILLFAFG
                     IFSAGFLPAFSVAKELCNKKYVATGLSFMNMMNMVGIALAQPLIGYILDKMWQGQMMG
                     TVRVYPLEAYYTALSILPIGMLVALLILPKIKETYCQSVN"
     gene            26198..27541
                     /gene="miaB"
                     /locus_tag="I9760_000480"
     CDS             26198..27541
                     /gene="miaB"
                     /locus_tag="I9760_000480"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAT5042275.1"
                     /translation="MVKKLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVI
                     LLNTCSIREKAQEKVFSQLGQWREYKAKNPHVLIGVGGCVASQEGSDIIKRAPFVDIV
                     FGPQTLHRLPALLNERLEKNKSVVDISFPEIEKFDHLPAPRAEGPTAFVSIMEGCSKY
                     CSFCVVPYTRGEEISRPFDDVLAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADL
                     ALLIHYIAAIDGIGRIRFTTSHPLAFSENLINAYAEVPELANHLHLPVQSGSDRILSL
                     MKRGYTALEFKSKIRKLRKIRPDIRLSTDIIVGFPGETDKDFQDTMDLVHEIGFDTSF
                     SFIYSPRPGTPAANLPDDTPMEIKKQRLQILQNRLLMNAARYSESMIGSKQKILVTGF
                     SKKSSQQLSGRTECNRVVNFDGPPHLIGQFIDVQISDALPNSLRGRLLEKEMQPA"
     gene            27541..28506
                     /gene="rluD"
                     /locus_tag="I9760_000481"
     CDS             27541..28506
                     /gene="rluD"
                     /locus_tag="I9760_000481"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAT5042276.1"
                     /translation="MIEHIQQQLSVPRELHNQRVDSVLARLLPEYSRSLICNWIKTGA
                     VTLNNLPCKPKDKALGGDLITINVDHTIVNTDFNYCKPENIPIQIIYEDSEVIVVNKP
                     AGLVVHPGAGNKEHTLVNALLHHCPELQLLPRAGIIHRLDKDTTGLLIVAKNLISHTS
                     LIRQMQNREIKRHYLTLVHGYVISGGTIDTGFGRHPKNRLKMAVTDFGRQAVTHYNIN
                     KHYKDFTLLNVSLMTGRTHQIRVHMNYINHPVVGDQLYGGRMKFPPNADEAILSVLKQ
                     FKRQALHACSISFCHPVKNTEITLNALLPDDFELLLNTLDQHDEK"
     gene            28496..29221
                     /gene="pgeF"
                     /locus_tag="I9760_000482"
     CDS             28496..29221
                     /gene="pgeF"
                     /locus_tag="I9760_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HAT5042277.1"
                     /translation="MKNRFANWPAPKNITALSTTRLYGFSDGPYASNNLGLHVGDNQQ
                     HVFKNRQQLIELIDLPAEPIWLEQTHSTKCITVETEKTRDADAAITRLPKLPLVIMTA
                     DCLPILLCNRQGTEIAAIHAGWKGLYNGIIENTLKKLNSNRTDIIAWIGPAICNKCYE
                     VGEEVYLSFTNKYSLTRNAFEPVHSKWLADLPQIAEIVLNLEGVKSVFQSNLCTFELK
                     NEFYSYRRESQTGRIGTFIWINE"
     gene            29238..30614
                     /locus_tag="I9760_000483"
     CDS             29238..30614
                     /locus_tag="I9760_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HAT5042278.1"
                     /translation="MITRIKLFIASLLVFFIPVADAAQDLTNMPSLAPVLKNAMPAIV
                     NVAVQGYLPNNMASGNADDDDGENSKQPSRIPEKGRKFESIGSGVIIDPKNGIIITND
                     HVIRNANLITVTLQDGRRLKARLIGGDSETDLAVLKIDAKNLKSLVIGDSDKLEVGDY
                     VVAIGNPFGLNSFGNSQSATFGIVSALKRSDLNIEGVENFIQTDAAINPGNSGGALVN
                     AKGELIGINTAIISPYGGNVGIGFAIPINMAKDVAQQIIKFGSIHRGLMGIFVQHLTP
                     ELAQSMGYAEDFQGALVSQVNQNSPAQLAGLKSGDVIVQINDTKITQATQVKTTISLL
                     RAGSTAKIKILRDNKPLTLDVEVTDIKKHEQKLQSNNPFLYGLALRNFEQESPPHGNV
                     VGVQVVGASETSAGWRAGLRPGDIIISANKTPVKDIKSLQAVAHEKGKQLLVQVLRGA
                     GALYLLII"
     gene            30922..30998
                     /locus_tag="I9760_000484"
     tRNA            30922..30998
                     /locus_tag="I9760_000484"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30956..30958,aa:Met,seq:cat)
     gene            complement(30955..31155)
                     /locus_tag="I9760_000485"
     CDS             complement(30955..31155)
                     /locus_tag="I9760_000485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042279.1"
                     /translation="MMQDDLKYYFWAGPDLIAVAILLLEVTNERQKSGVGLDCSLQVF
                     SIRGHMMEMVAMGGLEPPTSAL"
     gene            31200..32348
                     /locus_tag="I9760_000486"
     CDS             31200..32348
                     /locus_tag="I9760_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HAT5042280.1"
                     /translation="MDFILFRINLIRYVVLLFIITLNLDCISVAFSSQIIYGTQNAAR
                     VTGKNLKRIYFLQLGAFSQKNNAQQLARKIQARTQYPVIISHSGNAFSVRVGPINTQE
                     LHQLSIKLSQNSNITPIKHNSSPKQTPTTQTKSQILVKETSRPLPTHSVTKETIDGKR
                     SWYVTAKVGAQETTPDDQFTVNNDSGFPSPFDQDIYTNSSDSSLLLGIGGGYRFTNII
                     LPAISVGVYYSHFFNMDINGQVIQYSLPEFTNYNYQWKVKSDLVLASAKANVISWKNL
                     MPFVQGGVGVSSNRASQYYESALPDVTPRISPNFANHTTTVFAYQLGAGLDWEIRPQW
                     LLSLGYEYSDLGKVRSGYGTDSWNRETLSSKNLTSNAGTLGFTYLFHG"
     gene            32401..34866
                     /gene="legT"
                     /locus_tag="I9760_000487"
                     /pseudo
     CDS             32401..34866
                     /gene="legT"
                     /locus_tag="I9760_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegT"
     gene            complement(34946..35260)
                     /locus_tag="I9760_000488"
     CDS             complement(34946..35260)
                     /locus_tag="I9760_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HAT5042281.1"
                     /translation="MVIYEVNLTIDPEIFVQFQAWLKKHVAEIIQFPGFMQACILKQE
                     KETKSAKEKLTVQYQLDNGDSLSRYLSELAPQMREDGIHLFQTKFSADRRIFEVQEII
                     SK"
     gene            35648..36733
                     /gene="lbtU"
                     /locus_tag="I9760_000489"
     CDS             35648..36733
                     /gene="lbtU"
                     /locus_tag="I9760_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072401887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore legiobactin import outer membrane
                     protein LbtU"
                     /protein_id="HAT5042282.1"
                     /translation="MLYIRNYIRTRIILGILMLNVAGLAFADAGDSLFKNEKLTYDGH
                     LFFNAANSAIQSEKYLLNQQLSNIKMGSELQVTDWNKMKGLLIYNTLPTPVAPQFYFE
                     QFYDELQIESSNIFFAFGKKWLAFGNYKSDLIYKPLTKALGQTNEVAAIIGYDSLYYA
                     NASFFKPYSRISTSSLPLYYNLNTGVHNQSMDAGVSYLYSLAESQLFQYNKGFGGLLS
                     QSLKTRVPGFATYFNLKYKKTSTYLTYVTAITPFALEDMSYNNRGASPKALSIQNSYD
                     VNIKKFSFKIIGFYDYTFQALALGIPKQRLGLGLSATPFPYLGIQFQYSRDYSYRNNA
                     IASGINHFVNGRNTKMNNLALQTILNF"
BASE COUNT        11785 a         7450 c         6730 g        11037 t
ORIGIN      
        1 gctctatggg aagaaggaat tgatgttgaa gaggttaatt ctgccaagaa agatatatcc
       61 aaacctgctc cagaaatcca aaagttaacc gatcttcaag aacaattaag aaatcataaa
      121 gaatcaggag taaaattatc atctgaactt gaagaaactc ttaattcttc tgtaaaaatg
      181 tggaaaacca aaattgactc tccatgccaa gttatatcag aatcctctgt aaaagcactc
      241 gtaagtaaaa taaattcaac tcgtcccgag cttgtaaaag aaaaggaaca gttgccagaa
      301 caagaagaat ctctaagtaa agaagcaaaa aaggcacaag aagagttaat aaaaatacag
      361 gaattttctc aattttacag tgaaaattcc agtgcttata tggtaattgg tttaccacct
      421 catcatcaag tgtctttacc tttagctgta aatggcaaaa gggggttaca tccagaagct
      481 atgttaaaaa aaatgcatga gcttgtagct ggtccagaaa aaaaagaatt caatttacat
      541 accaataact gctcacttac ttcgatagaa gtattatctg cgggagcaca acatgatcca
      601 ttattgcatt caataatggg aacacgtgcc ttaggttttt ttggaacacc acagcaggta
      661 ttagaaaatg ccaaactaac aagtaaaacc attaatgaag ggaaaaaaag taacattttt
      721 acaccattag ttactgcaag tccattagac agagcattag gatatgccat gtcaatatac
      781 atggatcctg aagcctcaaa agcaaaacaa aatgccggcc tggctcttgg tgtactggtt
      841 ggtttagcca aaacaccagg cattatcatt ggctctttgc tgaaccccaa acaaggtttt
      901 aatgatattt taaatacttt aaacctcgtc tatagtcgca attctactgg attaaaagtt
      961 ggattaaccc ttatggccct accagcaatg atagtattag ctcctttagc agcaatacaa
     1021 aaaggtgtgg aagttattgc tgaaacgata gccaagccat ttaaacttat agcaaattta
     1081 tttaaacaaa agcctgaaag cactgatgag attactgttt ctgtaggtag taaaaaagta
     1141 gctgaaaagg aaggtagtta ttctaatact gcgttagctg gattggttaa tagtaaaata
     1201 aaatcaaaaa ttgatgaaaa taccattaca gtagaatttc aaaaatcgcc tcaaaaaatg
     1261 atagaagaat ttgaatcaca attaaaagaa aatcctggaa aagttgttgt attaagtgaa
     1321 aaagcacata atgccgtcct aaaatttgta tcaaaaagtg atgatgaagc actaaaacag
     1381 aaattttatg attgttgtaa ccaatcggta gcacgtagcc agaaattcgc acccaagact
     1441 agggatgaaa ttgatgaatt ggtggaagaa gttacatcta ctgataaaac ggaattaact
     1501 acaagtccca gacaagaacc atcgatgagc tctactattg atgaggaaga aaacatcgat
     1561 tcagaacatc aaatagaaac tggaactgaa agtacgatgc gcatataaat aataatgata
     1621 atcattattg tttatatgtt gtttactact tggttgtttt ataatcaagg gagatctaaa
     1681 aaattatttt gttagaatac ttcataattt ctccaaaaag aatccagcac atttatttat
     1741 tatagtggag gtaataagtt atgccaaaat taattttatt aggagactcc actcttgata
     1801 attttaattg ggtgccaaat aaagctactg taactgatca cttaagacgt caattaccaa
     1861 aaatggaaat tcttaatttc gctgttgatg gatttacaac tgactcaata ttatatggag
     1921 aatataaaga ctgtgccgtt acttcatccc aacataccca caaatatttt gaaccactta
     1981 aggcgttaga aaatgaacag gatgttgaac atatagtttt gagtgttttg ggtaatgatt
     2041 ttcgcattca gttaagcaag ctaattttta tggctccaga agagagacca aaagctattg
     2101 agcaattgat aaatcacata ataaagaact atattcaagt tgtgaaagaa attagaaaag
     2161 ttcaacctaa tgcaaaatta agtatagtat tgcaatacac accatatgta gaaaacgacc
     2221 cttatctaat ttatttttta atggataaac tttttcatca cggaacggtt tctcatggtt
     2281 ttttatccta tttacaaata atgtattatt ctatatttgg tttaaattct aaacatgaac
     2341 aacaggcgtt aaacctgctc cataaactaa tggcaaaagt atatcgaaac gttttcaatg
     2401 aacttggtga caactctttg gccattattg atcttgccag tagttttgat tatcgaaata
     2461 gcaaattata taaagctcaa attgaaccta gcgatgaagg aggtaaattg attgcctctt
     2521 taattacaca tacgattaca catcatgact ttcaaaatga atccatgctc tatgccaaac
     2581 cacctttaca gcctaatccg aaaatactgg cagtcccttt aaccaaactt aaaaaagatt
     2641 ggtttcctca acaaatatat gtaactcaag aacaagcaat aactgctttt aaacaagctt
     2701 atttgttaca gctaaataaa gatagacaat cttatttagg tctatatagt ttttttgctc
     2761 gcagtaatgt agattttgaa cacgcaactt tagaaaaact tatagtacat gcgcaaaact
     2821 attcaactaa aaaaacaggg tatcgcacat taaaagttat gcagaatcta ggttggttga
     2881 cggagcaagg aaatataaaa gaagaaagtg ggatagtaag gctattaaaa aattgaacta
     2941 tatgtagtag ttcaaatctg atgatagtaa ccagttcggg gcgccaagca aaacttagtg
     3001 cccgctaaca ttatagattg aattaaaatg atatatttat agcgattcaa atatcgcagc
     3061 agctcccatt ccggttccaa tacacatggt taccataccg tagcgccctt ttgttcttct
     3121 caatccatgt agtaatgtag ctgttcttat ggtgcccgtt gctcctaaag gatgccccaa
     3181 agcaatagcc ccacctaaag ggttaacttt ctctctgggt aggtcaaggt ctttaataac
     3241 cgccaaagct tgggcagcaa aagcttcatt caattcaatc caatccaatt gatctaaagt
     3301 aattccagcg cgctttagcg ccataggaat tgcctttact ggtccgattc ccatgatttc
     3361 aggcggtact cccgcaacgg catagctcat taaacggcct aaaggtttta aatcatactg
     3421 ttttaaagcc ttctcactgg ctagtaatgc cactccggct ccgtcactcg tctgagaact
     3481 atttcctgca gtcactgtcc cgtttaaagc gaataccggt tttaatttag aaatcgcttc
     3541 gtaagatgtg tctgctctag ggccttcgtc cttatttatc aattttttct ttatttcaac
     3601 ctttgaagtt ttcagatcag ggtatcttat agatatttcg attggactta tttcagcgtt
     3661 gaaatcgagt ctttcctgcg cagcaattgc ttttttatgg ctttctatgg cgaattcatc
     3721 ctgttgttct cgagtaattt cccaacgctt tgcaacgttt tctgctgtaa tccccatgcc
     3781 ataagctata gcaacgtgct catcattaaa aatggatgga ttcgccgtgt atttatttcc
     3841 acccataggt accatggaca tactttcgac accgccacct agtgctaaat gagcctcctg
     3901 attacggatt gtatctgctg cagtaactat actttgtata ccagatgagc aaaaacgatt
     3961 aatagtcatt gctggaacag attgtggaag atcagccaat aaagaagcga ttctggctac
     4021 attcatacct tgttccgctt ctggcattgc acaacctatt actacgtcag ctatctcttg
     4081 ccaatcaatg ttttgatgtc tatgtattaa acatttaata gtatgagcca ataaatcatc
     4141 aggtaatgtg tttttaaaaa cacctcttgg tgctttacct actggtgtac gtaatacgtc
     4201 aactacatat acgtcagtca tcctaattct ccttgcttag ttgcgcagtg gtttaccagt
     4261 ctctaacaga tgactgattc tggcttgggt taatgatgta ttggctagtt ttatgaatgc
     4321 ttctctttcc aactttagca tccattgttc atcaaccaag gttccatgat tgacatcccc
     4381 accacacaac actttcgcca attcattcgc cagaaaataa tcatgctgtg atataaatcc
     4441 accttccaac caattcacta aaccggattg taatcgggca tgcccctcta tacctgccac
     4501 tttaaatcgt tgttttttca taggaggaag ataattagca gctcgcatgg ctttgatttt
     4561 ttctaaagag gcatataaaa cctcatcagc atgcattaga atagaatcag ccgcacgcag
     4621 atatcccata tttctggctt ccaccgcact tgcagctact tttgcagttg ctatttgctg
     4681 aaaaactggc aatataaaag ttaataaatc agcttgttgg gcatgatgtg ctgaacgcat
     4741 agcgagctct ttgcaaccac caccagcagg tataactcct actccagcct caactaaacc
     4801 gggataagat tcaaaagcag caacaataga atcacaatgc atcattaatt cacaaccacc
     4861 tcctaatgct cgacctctta aagcagctac agttggaatt gaactatatt ttaaccgcat
     4921 tgctacccgt tgaaattgct ctaccatatg ctcaagagct tgtattgttc catctgttaa
     4981 taatttggag acgccacgta gatcagctcc tgaactaaaa ttggatgcat cttgttgata
     5041 aataattaat ccctggtaat ccctttctgc gatatccaaa gcagtctcta tgccatcgag
     5101 aactgattga ccaattgtat tggctttgct tttaaaacta atcacagcca catcatcttt
     5161 taagtaccac aaacaaacac cctcattttc ataaataaca tgaacaggat gatgtgactc
     5221 ctttaatacc ctgtcatgga agaattgttt atggtaaaca ggtaactgac ttctggcgtg
     5281 ataattgttt ttaccaggcg aataagcgcc ttctcgtgtg taaaattcct ttatttcata
     5341 taaccactca ggtagttttg cgcttgataa tgccgtatta tcctgtattg attcatcgat
     5401 ttgtcgagtc acactgtgta aatcagaaag ctgccaagtt tcaaatggtc cttgcgacca
     5461 tccaaatccc cagcgaattg ccagatcaac atctcttgca ttatcagcaa tttcttccaa
     5521 atgatacgca cagtaatgga ataaatctct aaagcaagcc gacaaaaatt gagcttgctt
     5581 attatttgat gccattaaat tcttcattcg agcttcagga tctttaatat ccattatcgc
     5641 tttcagttca tcactcactt ctgctcgaga gggtctatat tgaccggttt gaatatcata
     5701 aacttctatt actttacctg cttttcggta aattccttgg ccagattttt gccctaaatg
     5761 accttcctta attaaaccat tcaaccagtc cggcagaata aacattttat gccatggatc
     5821 accagttaat tgagactgca tggtattaat aacgtgttgc atagtatcca aaccaaccac
     5881 atccatggta cgaaaagttg cagatttcgg tcttcctaat aatggaccag tcaaggcatc
     5941 cacttcatca attcccatat tcatggctaa agtatgatgc aaggtagtta atagtgaaaa
     6001 gactcctatt cgatttgcga taaaattggg agtgtctttc gctctcacaa ctcctttgcc
     6061 caaatactcg gttaaccatg tttccaaata atcgagcaaa tcagatgatg tagtattggc
     6121 aggaattagt tctgccaaat gcatgtaacg cggtggatta aaaaaatgga ctccacaaaa
     6181 attggatcta tgctgcgcag gtaatacctc acatagctta ttgatactta gccctgatgt
     6241 attactaact aaaattgttt tatcagataa ataaggcgaa attcgcttgt ataagtcctc
     6301 tttccaatct acacgttccg ctatcgcctc tatgactaaa tcacaagaag ataactcttc
     6361 caaattttcc gcataatttt ttgcttgtaa taactcattg gtttggggag ttgcaagagg
     6421 agttggtttt agtttaccta aattggcaat agctttatcg accaagctgt ttttaggccc
     6481 ttctttgcca gctaaatcaa acagtaaagt ctctattccg gcattaacgc agtgtgctgc
     6541 aatttgagca cccatgacgc cagcccctaa aactgcaact tttttaatga aaaaaggctc
     6601 ctgcattgtt attatcctct tgttaataat cgattgatgc ctctatgagt cagatttaca
     6661 ggtaacaggc acttaccatt ggctaaaagg agcaataatc tgttgatctc gcccctttct
     6721 acatcttaac ttaaattaaa acgaataccc tgagccaagg gcaattcatc tccccaatta
     6781 attgtattgg tttgcctcct catataagct ttccagctat ccgaacctga ttcacgtcca
     6841 ccaccagttt ctttttcgcc tccgaaagca cctccaattt cagcccctga agtgccaata
     6901 ttaatattgg caataccaca atcacttcca caagcactga gaaagcgctc tgcatttttt
     6961 aagttttggg tgaataaggc tgaagaaagc ccttgaggaa cgccattatg taaagcaata
     7021 gcctcatcca gtgtccgata ggacataaca tatagaatag gagcaaaagt ctcttcctgc
     7081 acaatatccc aatcgttttt tacatcacaa actaaagtag gctgcacaaa ggagccagct
     7141 tgttttaata tttccccacc ataaacaatt tgacctcctg ccgctttaat tctatttatt
     7201 gcttttttaa actgttcaac agcttgcagg tcaatcaaag gacccattaa atttcgtgta
     7261 tcgagtgggt caccaatagt tatctgctca taagcatgtc ttagcctttt gataacatcc
     7321 tggtattttg attcatgaac aaacaggcgt ctcgtagtag tacaacgttg gccagcagta
     7381 cctacagcac cgaatactat ggcaggaata gccaggttca aatcagcaga ctcatcgagg
     7441 atgatgccgt tattacctcc caactccaga atgcttttac ccagtctggc agcgactttt
     7501 gctgctactt gctttccaac tgcagtagag cctgtaaagg atattaactg tattcgttta
     7561 tcatcaacca tggcttcaac cacatcgtga gaattgggaa tgatcaagcc aaatatttca
     7621 ggacaattat tttctttcaa tacctgattg caaatatgtt gtactgccac tgcacagaga
     7681 ggtgtttttg ccgatggctt ccatatagta acgttgccac aaatagcaga taaaaatgca
     7741 ttccatgacc atacagcaac aggaaagtta aatgctgaaa tcaccccaac aattccgtat
     7801 ggatgccatt gctcatacat tcggtgattt gggcgttctg agtgcatgga attaccatac
     7861 aacatgcgcg attgccctac agcaaaatca gcgatatcaa tcatttcctg aacttcgcca
     7921 tcaccttcct gtttggattt acccatttcc agcgatacca gacttcccaa gctatcttta
     7981 ttttctctta gtacttgccc tatttgcctg atgatttcac cccgctttgg agctggcact
     8041 tttcgccagg cttgcgctgc ttgctctgct cgctgcataa cctgctcata atcatccata
     8101 gtgcaagttg ctatttcagc aattttattc ccagtactgg gattgaagga ttccagagta
     8161 tgggcatggt tgacactatg ccaaccatgt ccactaaagg ctccggggtt tactgattta
     8221 atatttaagc gttgcaataa atccataaga ctctcctagg cataatattt gccgaagcga
     8281 ttttccagca cgtgggacaa tttaaatttt tcttgcaaaa taagcccttt atattcattt
     8341 ccctgtccca ataccaaatc aacaacagcg catacgcccg aagctgtact gacttgaata
     8401 gctgaccact ccaaacctcg aattacttca gggtaaattt tcttgacata acttttttcg
     8461 gttagttcgc cctgcttaat accctcaaca gtaacataaa ctatcacgat atcctgatat
     8521 gtttttggaa ttgcattttc cagtattctt tttaacgtcc ctcgatcttc atttaatcgt
     8581 aagtcattca ttaaaaagcg cattttttta caatgacctg gataacgcat ggttttgtaa
     8641 ttcatcgatt gtatttttcc agcataaagt tcagccaagc ttcccaaacc accggacgtg
     8701 ttgaacgctt catattcaca accatcaatc tgaattgact ccaatccttc cagcggagcc
     8761 ataaccacat ctttaccacc ttcaatacca tagcaaggat taccatattc attaataacc
     8821 ccatcagtag accaggttaa tgaataatga agcgcattat tagcacgctg tggcaatgct
     8881 ccaactctta gtctggcatg atgacatttc tcaaattcct gcatcaaact attagccgca
     8941 atactgataa agccaggtgc taatccacat tgaggaacaa aagctgtctc ggcattttga
     9001 gcaatagctt ttactgcctc cgttacagaa gtatcttccg ttaaatcaaa ataatgagct
     9061 ttggcagctt tggcggcttt ggcaacatga gtatttaaaa aatagggaag actcgaaatc
     9121 actgcgataa tattgtgctt ttgcaaatag gcctgagtgg attgttcatc tttaacatcc
     9181 aatgcaaccg ttttaatctc aggcagcgca gttaataatc ttgtcacatc agagccatta
     9241 aattccagat cggccaaatg aacctgataa gacccactat cggcgagtag acaagcaatt
     9301 agactgccta ttttcccagc accagtaatc attacgttgt acatacaatc cttctccttt
     9361 gtaaaacttg ataatcctac acaaattatc agagattaga tagatccaaa acatgatcaa
     9421 tacagcaatt cttgatatca cttgaccaat tagtcttact catttatctt gaccacttta
     9481 atttagtcta gttgtgacat gaagcaggcc gctttgatac attcgacaat aataaacaca
     9541 tcaacactaa accaatattc gaagttcgtt gttgtatttt tagcaggttt attagccccc
     9601 ttggggtttg cacctttcca tctgccagga ttaactattt tgagtctggc cattttattt
     9661 tctgaatttc ttcaatgcac ggttaagcag ggattttccc ttggtttcct atacgggtta
     9721 ggatactttg gcttcggagt atcatgggtg attatcagca tccacgacta tggacaactt
     9781 gattactttc ttgctggcgt tatcacctta ttgtttatca tgtatctttc tctgtttcct
     9841 ggaatagtcg gttattcctt taaactcctt tcaaccaagt gtaatttatt aacatgcatg
     9901 cttttgttta gcactctttg gtgtatagct gagcttatcc gttccaagct ttttactggg
     9961 ttcccctggc tattagcagg tataacccaa atcgatacac cattgagata tattgcacca
    10021 gtagttggtg tttatgggct tggattaata tgttcttttt tgggtaccct actggttctg
    10081 ataattaaag acaatacgat taaacgatat tactacctta gcgtttttat cttgattctt
    10141 attgcaccat cactggggaa aaatatccat tggacagaaa tcaagaaaga acctatttct
    10201 gtcggtgcaa tccagcctaa tctctcaatg agagacaagt gggacgaaac tctcttttgg
    10261 gatttattaa aatactatga aaacaaaaca gataaactgt taggaaaaaa tttaattgtt
    10321 ttacccgaat cagccatacc cctaccagca agttatgttg aagattacct acagaaactg
    10381 catgaaaaag cattaaaagc caaaagcagt ttaattttgg gaatcttgca acctacagat
    10441 aatgatgaaa ccaattttta caattccatc attagcttgg gggaagctac aggagcctat
    10501 gtgaaacgcc aattggtacc atttggagaa tatataccaa aaccctttat ccccatcaat
    10561 cgttggttaa atcttccaga acctaatgtt gttcccggca gatcgaatca gccattaatt
    10621 aacatagcta atcatcccat tgccagtcta atttgctatg aaattgctta cccaaatatc
    10681 ttgagatcac aaatgcctgc agctcaatgg atagtctcta tcagtgataa tggctggttt
    10741 ggccattccc tggcaagcta ccagcaatta caaatggctc aaatgctctc tttgcttaca
    10801 ggacgatacc aaattctggt gaataatgat ggactctcct cagtaattaa ccaccaagga
    10861 actattgtcg aagggctacc agcttttaat tcaggaatgc tccaaacaga tatttttcct
    10921 gcaaatggca tcactccctg gataatttgg agtgactatc ctacttttgt tttttgtttc
    10981 atatttgttc tttttgtttt gttttttaat attaaacgat ttacagaaaa ataatctatt
    11041 gctggcaaag gcaagagggg ttatccttag cagttccgtg ttattaaatt tattggccta
    11101 tggataatac ctataacccc caagaagttg aagaacaagc acaacaatat tggcataaga
    11161 agcaatcatt taatgttact gaagatttaa acaaagaaaa gttttattgc ttatccatgt
    11221 ttccctatcc cagtggcaca ttgcatatgg ggcatgtgag aaactatact ctcggtgatg
    11281 tcatagcccg atatcagcgg gcattgggaa aaaatgtcct gcaacctata ggatgggatg
    11341 cttttggcct accagccgaa aatgcagcca tcaagaataa aattcctcct gcagaatgga
    11401 ccaggaaaaa tattgctgca atgaaagagc agtttttacg cttgggaaat gcctatgact
    11461 ggaaaagaga aattactacc tgtgatcctg aatattatcg ctgggagcaa tggtttttta
    11521 ttcgtttatt tgaaaagggc ttagtttaca agaaaaacgc tgttgttaac tgggatcctg
    11581 ttgatcaaac agtattagct aatgaacagg tagttgacgg tcgcggctgg cgttcaggtg
    11641 ctttggttga gcgcaaggaa atttctcagt ggtttattaa aattacctcc tatgctgatg
    11701 agcttttaag ctctttagat agcctggatg aatggcctgc acaagtaaaa caaatgcagc
    11761 gcaattggat tggcaaatcc ataggcacag aaatttactt caatgtaaac aattatccca
    11821 aacgattgaa aatttacact actcgcccag atacattgat gggagcaacc tatcttgctg
    11881 tggcaacaga tcatccatta gcaaaagaag cggcttccaa caataaaaag gttcaagaat
    11941 tcctcgatag ttgtcaagga attaagatag ctgaagcaga actggcaact atggaaaaac
    12001 gagggattga cactggaatg actgcaatcc accctataac gggtaaggag ttacccatat
    12061 gggttgctaa ttttgtttta atgcaatatg gttctggtgc agtcatggca gttcctgcac
    12121 atgatcagcg cgattgggag tttgcgcaaa aataccaatt acctgtcaaa caggtaatca
    12181 agcctataga cattgagcat gactttaatc aatcagctta tactgaagaa ggaattctga
    12241 ttaattcaaa tcagtttgat aatctactct ctagtaaagc cattcaagtc atcacaaact
    12301 ttctggaaga aaatgacgct ggcaaagcta ccattaatta ccgtttaaga gattggggcg
    12361 tttccaggca aagatactgg ggaaccccta tccccatgat tatctgcgaa caatgcggta
    12421 ttgttccagt ccctgacgaa gaattacctg ttgttctgcc agagaatgtt gattttaccg
    12481 gtaccggctc tccacttaca caatgcaaag agtttgttaa tgtaacatgt ccaaaatgcg
    12541 gtcaagatgc aactcgagaa acagatacat ttgatacctt tgtcgaatct tcatggtact
    12601 atgctcgctt tgcttgcaaa ggtcaggaaa atgccatgct tgatgaccga gccaaatatt
    12661 ggacaccagt agaccaatac atcggtggaa ttgaacatgc agtgatgcat ctgctgtacg
    12721 ctcgattttt tcataaattg atgcgcgatg aaggattagt taattcggat gaacccttta
    12781 aggctttatt aactcaaggt atggttttaa aagacgggca taaaatgtca aaatcattag
    12841 gtaatgtggt tgatccaaac catcttatca atacctacgg agctgatacc gctcgcttat
    12901 ttgtcatgtt tgcatcccct cccgagcaat cactggaatg gtcagattct ggagtcgaag
    12961 gagcacatcg ctttttgaaa cgagtttggg ctttttcaca tcagcataga gacatgctca
    13021 ttgacattaa cgatagtatt ttgagtggaa atggtcatgt cgactggaag gaagcggagt
    13081 ctcgattaaa aaaatcccgt catattgtgc atcaaatact tgctcaggca acccatgact
    13141 atgaccgaaa tcaatttaac acggttgttt ctggctgtat gaaattattt aatgaaatat
    13201 ctgattactc cattgaaact gaaaatgata aattttttat acactcaagc atcagtatat
    13261 tgctgaggct actcgctcca attaccccgc atatttgtca ttgcctttgg cagcaattag
    13321 gttttgataa agcaataatt gatgcgccgt ggccaaaggt ggataaaagt gctctaaaaa
    13381 ctgatgaagt agattatgtt gtacaagtta atggaaagct cagagctcaa tttactgcaa
    13441 gcactgatgc gactgaagag gaattaattg ctgcagctaa ggaacatgcc cacaactttg
    13501 tggtaaacca caccattaaa aaagcaatta ttgtcccaca taggcaacta atcaacttgg
    13561 tcattggata aacatgtgtg attcgtccca gcaatctcta aatattttac acaatttctc
    13621 aagttatggg tggaattacc tataaagtgc aaataagaga gggaaaagaa gaatgactca
    13681 gatccacaag tacttagcat ctcttatact gatttggctg ctaactggtt gtggttttca
    13741 tttgcgtgga gcaattgata tccctgagtg gcttgataat gtttctatta tttccaggga
    13801 tggaaataaa gaactggttt ctatactcaa gtcccagctg gaaggctata aaattgatgt
    13861 caattctgac ccatctctcg cgaaatactg gttaattatt aacagttcca gtttacagca
    13921 gcaaattgtt agtattggtg caagtaccaa tccaagacaa tatcaattaa tcttaaccat
    13981 tgaatttatg ttgcagactc ccaaagggca aattattaaa acccccagaa aagttaatgt
    14041 gagccgtcaa ctcaccgtga ataatgacag gatattaggg tcaaatgaag aacaagctct
    14101 tttagtcagc gaaatgaggc gtgatgccgc tattcaaatc attaatcgtt taaaccgacc
    14161 atgatatgca aataaatcaa caagctctta ctcagctttt acaaaaaaaa atacatcatc
    14221 ttaatgtact aattggacaa gatcattatc ttattgacga agcactcaat aacataaaat
    14281 caatgcttaa aaaatcatct gaatgtgatg agaaaatcct ctctattcag tcccctgaag
    14341 aatggcatgt cgttctggaa gaaacaaata attactctct tttttatcaa acagttattt
    14401 taaccatttt ctttgataaa aaaacgatag atacaacggg aaaaaaaatt cttagtgaat
    14461 atctcaaatc agccaactct catagttata ttattatcag agcccctaac cttaccgcca
    14521 agcaacttca atggttaaca catgatgaac aagcgctgat tatcattgca caaccattaa
    14581 gctcagaagc aatgaagaga tggattaatg agcaattaaa attaaatact atctcttttg
    14641 atccatatgt tcctgaatta atttatcaat atactaaagg aaatatgcta gctaccgctc
    14701 aggtaattga aaaaattact ttgagcaata cacccggcac tctcattact ccttacctgg
    14761 ccttggaaca tttatcggat caatgtgatt tttccctttt tgaactgatt gattcatgcc
    14821 tacttggtca tagcgacaga gcaattcata ttttaagaca agctgccaat aataaaacag
    14881 aagtcacatt ggtattatgg atgcttaccc aagaaataag aacgctatta caattattat
    14941 ttctaatgca acaaaagatg gatatcaaaa tggcatctaa tcaattaaaa atttggactc
    15001 aaagagttaa tctttatcaa tccgctatta aaagattaaa ccctttactt ttacaacaac
    15061 ttcatcacta ttgccaatct attgatgata aaatcaaatc caactcaaac aatcaagtat
    15121 ggaatgctct ggagaacatt tctctttcct tatgtttagg gcaattacta ggtgatgcat
    15181 gcatagcata gcaatatttg gtggaacatt tgacccagtg cataatggtc atatcaagac
    15241 cagcttggca attcaagcta actttggctt tgattcctat tattttttac cttgtaaaag
    15301 ccccgcaatc aaacctccgt cctttgccag cagtgagcaa agagtagaaa tgctaaagtt
    15361 agcactcaaa ccttaccctg attttaaaat agatactcga gaactggatc gagatacccc
    15421 ttcttatatg gtatacactt tgcaaagttt cagacaggaa tacaccgact catcattaac
    15481 tctaattatt ggatacgatg gattattaaa tctaccacaa tggtaccaat gggaaaaaat
    15541 aatatcctta gccaatttac ttgttatcaa tagagaagag ttcttccaac agcctgtgcc
    15601 aaaatcagtg caaactctac ttaaccaata tagaaatgat gataaaaata tactgctaaa
    15661 tcatcatgct ggtagtatct gcttatacaa tgctggacac tatgatatat cctcaacaaa
    15721 aattagagaa caattaaaac aacataaaga tgtaaagaat aatttgcctg atttggtgta
    15781 cgattacatt aagaagcagg aactgtatca ataatatttt ctgttttatc aacagccttg
    15841 acctgcttta taaattcagg caaatggcta tatagcaaag ctacaacagg atcgaatcgt
    15901 gcataaagtt tggacttttg tgaatattcc tgataaggca aagaggtcat tttaacagca
    15961 accataatca gggcaacgat aaaaacgcca cgtaaaaagc caaaacccat tcctaaagtt
    16021 cgatcagttc cgcttaaacc agctcgtttt aagataaagc tgagtattgc attcactacc
    16081 cccccaacaa aaagggtggc aaaaagaata ataataaatc caatagctgt tcgtactgac
    16141 tgttcctcga tataagaact taaccaggga tccaggcttt gtgaataatt aaaacctagc
    16201 caaatagcta atacccacac acataatgca acaagttcct ttacaaaacc acgaaacaat
    16261 ccggtcaata cggacaaagc gataattgca acaattgcta tatcaaccca ttgtagttgc
    16321 ataactaact aactccagta ttcacaacaa aaccatttaa ttgcatggca ctagctaatt
    16381 gagtttttaa ttttattaca tcttttttaa cgggtgaatg gccaacaact actttataaa
    16441 ctattccatt tctgctatta gacttaagat aattcgcctt gtagcccttg ctgcgcaatc
    16501 gattaactaa agcttgagca ttagctattt gtgtaaatga agccagctga acagcataaa
    16561 tatcttgttt aactgctggt tttctcgcag caacaatttg gctaaccttt ttgctagtac
    16621 taacctttgc cgtaacaact ggagatgctt tcaccggtgg ttttgctgca gtcagggtca
    16681 ttactttctt tgctgtcgca ttcgctgctt tatttactgc caatttaatt ggctctgatt
    16741 taattgagtt ctctctttcc gcttcaagtg tggcgacttt agatgttctg atgagattgt
    16801 cttcttctgt gctgatgggt tctgcttttg ccaggttaat tgattttttt tcaactggta
    16861 ttgggggtat ttctgtctta gccactttta tagttttaaa tacctctttt tcatcagtaa
    16921 tcactacgtc aggaggtaat ggttttggtg gtaatttcac attgacacta aagttacttt
    16981 cagggctttg gcttgatttt tttattagtg caggagcaaa aattgccccc aatgaaataa
    17041 taacggccaa acctatcaag cgatgtttaa ctttctcatc cattatcagt ttcattgatt
    17101 ctccttctgc tccaaaagat tatgttgggc agccattaca tgactaaccg taaaaaatga
    17161 accataaaca ataattaaat cgtcatctga tgcttgcttt aatgccattt caaaagctgt
    17221 aaatgggtca ttataacaaa catctagaaa aatttctgct tccctgaata cagaaagcaa
    17281 ttcctcttga cttgcagcgc gcttattatg taattgtgca ggataccatc gattaacaca
    17341 atcccttaaa ggcataatca atccataaag atctttatcc tttaaagcgg agaaaacagc
    17401 atgaatttgc ccttttacat tgattttttt tactgtatca gcaagtaatc gaactgactg
    17461 cagattatga gatacatcgt acaaaatatc agtctttcct ttttgtaatt gcaaccgtcc
    17521 cgggatataa atgagctcca tcgctttctt tagattctta taggatacag gcaaatcatt
    17581 ttgcatcaaa taacaagcca ttacagctgc tgccgcagaa cgaagttgaa tttttggcct
    17641 ggtcaattgc tcaaatagct taccttgaca agtaaccccc caatggtctg cttcttcctg
    17701 ataaaaatag tctttaccgt ataaataaga tgatgctcca agttgtaccg caaactcaac
    17761 gatacttttt ggtggtgaga gatccgcata gataaaaggt ttattttttc gtagtattcc
    17821 ggctttctca taccctatca actcaagtgt gtcaccaaga aaactctgat gatcaaagtc
    17881 gatagtagta atgatcgcta aatcagtatc aacaatgttt gtagcatcca accgtcctcc
    17941 caagcctact tcaagaatta tcagatccag gtcatgctgt ttgaaatacc ataaggcagc
    18001 aagtgttgcc atttcaaaat aggttaaatt ggtagggtaa cgtgcttctt caatcacaga
    18061 aaaagcctta cataaatctt tatcttgaat cggtgttaaa ttaattcgaa tacgttcatt
    18121 aaacacaaga agatggggtg acgtataggt accaactcta aaacctgact ggtgatagat
    18181 ggtttcaagt gctgtgactg tagaaccttt tccgtttgtt cctgcaactg ttattacctt
    18241 gcaatttggt cgctgcaaat ttaatttctg cgctacagtc aaaactctgg tcaaaccaag
    18301 ctgtatttcc tgggtatttc tggtttccaa atgagtcaac cagtgattta aatcccattc
    18361 actccatggc gcgtgcaaaa aaactctccg ctcctttatg agtcaatttg gaaaccaatt
    18421 cggatatagt tgctcgaaga tgtttacgtt caactatcat atcaatatgt ccatgctcca
    18481 gtaaaaactc acttcgttga aagccttctg gcaaagtttg tctaacagtt tgctcaatca
    18541 ctcttggccc agcaaaacca attaaagcat tgggttcagc aataattata tcaccaagac
    18601 tgccaaaact agctgatact ccacccatcg ttggatcggt taacacaaca ataaaaggca
    18661 acccggtttc agcaaatttt gctaaagctg ctgatgtttt agccatttgc attaaagaaa
    18721 ataatccctc ctgcatgcga gcaccgccgc tggcagtaaa acaaatgtat ggtcgctttt
    18781 ctgttgcagc aacattcact gcccttacaa atttttctcc aaccgtcgct cccatggagc
    18841 cgcccataaa attaaactca aaggcactaa caaccactgg ttgctgcatt aaaaaccctt
    18901 taacaacaat taatgcctct ttctctcctg tcgctttttg cgcttgagaa attctatcct
    18961 tgtatttttt ggagtccctg aattttaatc tgtcctgtgg ctccagccca gcagccactt
    19021 cttcttgccc tgactcatct agaaattgag cgattctcaa gcgtgcggaa atgcgatgat
    19081 gaaaattaca agatgggcaa accataaaat tttttgccag ttcagtactg taaagcactt
    19141 cattacaacc agaacattta acccataaac cttccggaac ccccttcttt tgagaggttt
    19201 ctgtccttat tcttgatggt aacagttttt taagccagct cataaattag aacccagata
    19261 ttatcaaatc ggattattat acacagaaaa aaataatctg acatgtctat attggacttg
    19321 aatttcacat aaaggaataa gatattttac caaatcgact tgataaccag aaccaaatca
    19381 ccctgaaaac cccaattact cattaaaaat atccaagtcc atctgaagaa tttcagacgt
    19441 attgattata ttacaatcga tctttctatt tatttaacat actaatttta ttcaataatt
    19501 ttttgcaact tttcaaaaaa gcgctttcgg aactaaagtt tttttcacta tttccgataa
    19561 ctaaatcggg tacagtaaat tcacaacaat aaatcgaatt tactgaatta caaatgtccc
    19621 ataccaggga ttcaggtctg aggagactaa aattatggct caagtaatca acactaatgt
    19681 ggcgtcgctc acagcccaac gtaatttggg tgtttcgggc aacatgatgc aaacatcgat
    19741 ccagcgttta tcatcgggat taaggattaa cagtgccaaa gatgatgcag caggactcgc
    19801 catttctcaa cgaatgactg cacaaattcg cgggatgaac caagccgtta gaaatgccaa
    19861 cgatggtatt tccctggctc aggttgctga aggagcaatg caggaaacaa ccaatatttt
    19921 acaacgcatg cgtgagcttt ccgttcaagc tgctaactca accaataact catctgaccg
    19981 gtcttcaatt caaagtgaaa tttcacaatt aaaaagtgaa ttggagcgta ttgctcaaaa
    20041 tactgaattc aatggtcaaa gaattctcga tggttctttc tctggcgcaa gcttccaggt
    20101 tggggcgaat tcaaaccaaa ccatcaattt cagtatcggc agcacaaaag cttcttctct
    20161 tggtggtatt gccacggcaa caggaacaga agtagcaggt gcagcagcgg cagatattac
    20221 tatcgcaatt ggaggaggag cagcaaccag tattaactct tctgccaatt ttaccggggc
    20281 actaaacgga caagatgcta cgtcagccta tgccaaagca gcggctatca acgatgccgg
    20341 gataggagga ctatcggtta cagcgtctac cagcggcaca caagcagttg gcgcaatagg
    20401 tggaactgca ggtgatacct acaacttaac gattaatggt gttgcaatat acactaacct
    20461 taacgtagca acggcattaa ccaactctga tcttagagat gccatcaatg gcgtcagtaa
    20521 ccaaactgga gttgttgcat cacttaacgg tggtaatatg acactcactg ctgctgatgg
    20581 cagaaacatt acggtaacag aaagcggtac aggttttact gcaggtactg acggtttaac
    20641 tgtaactggt ggcgcatttg atggcgcgct acgcggtact ttatcaatca gcgccgttga
    20701 tactatcgcg attggtggta ctgttgctaa cattggttta agtgccaata tctctaaaga
    20761 cacagtaggg atagattcac tggatgtgag cacagcgtct ggcgctcaaa cagcgattaa
    20821 gagaattgat gcagcactca atagcgtaaa ctctaatcgg gctaatatgg gtgctctgca
    20881 aaatcgcttt gaatcaacga tagccaacct gcaaaacgta tcagacaact tatcagctgc
    20941 tagaagtcgt attcaagatg ctgattacgc tgctgaaatg gctagtttga ccaaaaacca
    21001 aattctgcaa caagcaggta cagcgatgtt ggcacaagct aatagcctac cgcaatctgt
    21061 attatctttg ttaggtcgat aggatgtcgc aatcgaagtg cagcaacaac tgcacttcga
    21121 tttctttata cctcggttga tgggtataaa aaggagtaag aaaaatgagt attgaacctg
    21181 tatcttcagt taataaggca gcccaaacag atcaaataaa aatagttgag gaagtctcta
    21241 agccagaaat taaaaatatg gtttatgatt ccgctttgga tgccgaaaca aaacaggcgc
    21301 tggataaagc aacaggtttg cttcaaacta taataacaga gaaaatatcc gataaagtat
    21361 tacgtaaaat gccttctgat gagtatttgc aattactaag cttgcttgat gatattatta
    21421 gtggttctat agacaagcac gtttaataat ttgaaatatt tattaaaaat ttgactagaa
    21481 caataacaac atagaaaagg gatactctat ggaactctct tcaccaggcg tagggtcagg
    21541 acttgatgta aaaaacttgg tcgatgcgat agtcacggca gaaataactc catcgcaagt
    21601 gcgtcatgat aaacagttaa acagtgtcaa tacagagttg tcggcgcttg gtcaattaaa
    21661 aagctattta actaacttgc aaacttcctt agccaagctc tccagtctta gccaattcta
    21721 cactatgaaa tcaaccatta gtgatccgaa ttacttttcc gctactctgg ggactggcgc
    21781 gactaaaaat acttatcaaa ttgaaataca aaaactggcg caacaacaca gtctggcaag
    21841 ctcttacatg actaatactg gaagcggaac aataaccata gattttggaa cctataacag
    21901 tgataaaacc acttttacac cgaatccttc cgctacgtca gtcaacataa ccattacgcc
    21961 tggcaacgat agccttgttg cggtacgaga tgccattaat aatactaatt caggaataac
    22021 agccagcata gttcaagata gccagggctc caggctaact atcacttcgt caaaaaccgg
    22081 cgaaaattat gccatgaaaa ttagtggtgg agttgcctca ttgaattatg atcctactat
    22141 gggagtgaat tcattaactg aaacagtagc agcacaaaac agtactgtta aaattaacgg
    22201 attaactttg gttcaaagtt ctaatcaatt gaatgaggca ataactggtg tcaccattga
    22261 tctaaagaaa gcagagattg ggaaaattgt caccttaact gttgatgata ataaagatca
    22321 gctcacaggc ttaatcaacg agttcataaa acaatacaat gacaccatga cttttttaac
    22381 caacttaact ggttataata atgaaaccaa acaaggagga gtatttcagg gcgaccctca
    22441 atttcgcaat ttaaaactca atttaaacaa gtgggcaact acccctttgg ctaaccagga
    22501 aggaccaatc cgctcattag ccgatttggg aatcatcacc aataaacaag gtttactgga
    22561 aatcaaacaa gacaagttta aaactgcact cgataatcac tataaagaaa taggagcatt
    22621 gtttgcaaaa acagccaagg ctaatgatac tggcataaac gtcaaatcca ttaattcaac
    22681 tgtcaaatca ggtacctatg atgtggtgtt gactgaattt actcctggag tcagcatgtc
    22741 tggaacaata ggaggcttat ctgctatatc atccgatggc attacattga caggaagtgc
    22801 tgatttgagt ggtctgtcga ttaatgtatt ttcggggtct gccggctccc gtggacaaat
    22861 aactgttaca gacggtattg cagtccaatt aagttccttg ctggatgctt acattgaaat
    22921 taaaggcgat ttggagcaga gaaaagatca actcaataat caagtcaagc aattgaataa
    22981 aatacaaaac gaaattgata tcaggcgcaa atcaattgaa actcgctatt taaaacaatt
    23041 cactgctctg gacactctac tcagccaatt acaaagcact agtgtttttc tatcccagca
    23101 attagccaat ttaccccaat taaaattgaa ataataggag gtaatgagaa tgaaaaacct
    23161 atattcacaa atttccagcc aatacaaagc catagagcta caaacccgca tagaaacagc
    23221 ctcccctcat gaacttatag atttattact tcaaggagcc cgatctcaca ttgctactgc
    23281 tcagggtaat atacagcgta accaaattaa agaaaaagga gaacatattg gcaaagccat
    23341 tagcatcatt gaaggtttaa aaatgagtct caatcatgat aaaggtggcg aaattgccga
    23401 aaatttactc cagttatatg attatgttca acaaatacta cttaaagcca atttaaaaaa
    23461 tgatgaagat ttattagctc agtccaatat gttattagct gaagtgcatc aagcctggca
    23521 agcgattaac tctaatgctg gtaatgaact ttaatatttg caatgagctc ccatgattca
    23581 gcatttgctt tttacagctt tatagcaacc aaaataagag ttttcttgat ttggcaatta
    23641 ttaaacccag tccatctatt tttattttcc caatgcaaat agtttaaggc ttctaaatat
    23701 caataagata tgaggaattg tctggaagtc ttgtagttat cgaaattttt gtacattttg
    23761 gtaatcaaga ataggattat aattttggca aatttgtata gatttatcga attggaatat
    23821 aaagtttttc cattatttgc cgccaaaaca gttaggatga ataaattaat ttcattatga
    23881 atacatttaa atcatcactg gcatttgtaa tttgtctctt acttggcatg caatcggcac
    23941 aggcgcaaat gcgatatgga gaaactctct gcaacttgcc agaatatttt tgcatgaaaa
    24001 taaaaagcgg ggacacttgg gataggcttt tccctaacat cgaagagcaa gatattgtca
    24061 gacgcattaa ccgaatgaat atcaggctca aacccgggat gattattgct gttcccaaaa
    24121 atatagagcg tttaaccatc tatgacgtct cgccttttcc aagatatatt gaaccaagtg
    24181 gtgaaaaaac aatttatgtc agtcaaaaaa aattggcatg gggagcttat gatgaagacg
    24241 gggaattgct ttggtgggga ccaatatcat caggtgttgg aaaatgtaaa gtaataggtg
    24301 gttgttctac accaacaggt tcatttcgca ttatccgcaa gcaagatatt gattgtgttt
    24361 caactgtttt cccaagaagg gcaaatggcg aacatggtgg tgcactgatg ccttattgca
    24421 tgcacttttt tagaggttat gctttgcatg gaagttatga agttccagga tatagggcca
    24481 gccacggctg tgttcgcatg tttattgaag atgcgcgttg gttaaatgag gaatttatcg
    24541 acctcccagg gggaggaatg agaggaacac gtgtaatcct tgatgcgcca gatgctcaag
    24601 agaatcccga taatctacaa taatatattg aaaacacaat caaaaactca ctcccaatag
    24661 acaaatagtg gtcaacctga taatatttta tttagtgaat tttactatga ggtgtgttaa
    24721 cgtataaatt cgctgtgtga ataaaataag tacattttta atcaaagatt agaatatatg
    24781 gaaataccat ctatatcctc ttttttgact aataactttc atagaatcta atgattggat
    24841 aaatttggag aatttttaaa taatggctca ttacaatgta caatccgaat cgctcaataa
    24901 agcaagtgtc agcttaatta tgccctggat ggtgtggggc ttgggttgct tattctattt
    24961 ttatgaatgc ttattgcaag tatccccaag cgtaatgagt aatgaactca tgagagattt
    25021 ctcggtaaca agtcatactt tagggattct atcgggcatt tatttctatt cttatgccgc
    25081 catgcagctt cctggtggtg ttctaatgga ttattttggc ccacaacgtt tattaactct
    25141 tgccacagtg gtttgtgccg tgagcactat agcctttggc atgacagata atttcttcat
    25201 ggcctgtatc gcaaggctga tgattggatt tggttccgca tttgctgctg ttggcaccat
    25261 gaaattggca gccaactggt ttccagcaca aaaattcgca ttattgactg gcttgatggt
    25321 taccataggc atgatgggtg caataggtgg agaaacgcct ttggctcttc taattgataa
    25381 ttttggctgg cgggaaagca tgattataat gggtagtgtt ggacttcttt tggcaacttt
    25441 gctcatcttg attgccagag atactcccaa gaatttttct cctgctatcc atcaacatcc
    25501 tgtcgaggaa gaacgtttaa tcccaagttt attcacattg ataaaaaata agcaactttg
    25561 gctagtagct acctatggcg ggttaatgta catggcaaca cccgtgtttt gcgggctttg
    25621 gggggttcct tttctgatga ataaaatgct aatagcaaaa tctacagcag ctaattttat
    25681 ctcattggtc tttgtgggtt gggcaatagc cagtccttta tggggtctgt tttcaaaccg
    25741 tattggatta cgtaagccgc ctatgtatat tggatgtgta ggggcaatga tttgttcttt
    25801 gctctttatt tttgctccca tcacttcttc aattactatg gaaatcctgc tgtttgcatt
    25861 tggtatcttt tccgcaggct ttttgccagc tttctcagta gccaaagaat tatgtaataa
    25921 aaaatatgtg gcaacagggc ttagtttcat gaatatgatg aatatggttg gaatagcgtt
    25981 agcacaacct ttaattggtt atattctgga taaaatgtgg caaggtcaaa tgatggggac
    26041 tgtgagagtt tatccattgg aagcttacta cactgctttg tcaatccttc caataggcat
    26101 gttagttgcg ttactaatat taccgaaaat caaagaaacc tattgccaaa gtgttaatta
    26161 atgaaataat accgcaattt caatttgtag aatcaaaatg gttaaaaaat tatatattaa
    26221 aacaaatggc tgccaaatga atgaatacga ttcttctaaa atggcagaag tactttacgc
    26281 ttcccatggt ttggtcaaaa ctgatcaagt cgaggatgcc gatgttattt tattaaacac
    26341 atgctcaatt cgagaaaaag cacaggaaaa agttttttct caactgggtc aatggcgaga
    26401 atataaagcc aaaaaccctc atgtgctcat tggagtggga ggatgcgtag cgagccagga
    26461 aggctcagac atcataaaaa gagcaccgtt tgttgatatc gtatttgggc cacagacatt
    26521 acacaggctc cctgccctgc tcaatgaaag gcttgagaaa aataaatctg ttgttgatat
    26581 cagctttcca gaaatagaga aatttgatca tttacccgca ccaagagcag aaggccctac
    26641 agcttttgta tctataatgg aaggctgtag taaatattgc agcttctgtg tggtgcccta
    26701 cacccgtggc gaagaaatca gcagaccatt tgatgatgta ttggctgagt gttatcaatt
    26761 agccagtcaa ggcgtcagag agattaattt actaggccaa aatgtgaacg attacagagg
    26821 tattatggac aatggggata tagccgatct tgccctgtta attcattata ttgccgctat
    26881 cgatggaata ggaagaatac gtttcacaac atcacatccc ttggcatttt ctgaaaattt
    26941 gattaatgcc tacgctgagg ttccagagct tgccaatcat ttacaccttc cggtacaaag
    27001 cggatccgat cgcattttat cgttaatgaa acgagggtac actgctttgg aatttaaatc
    27061 caaaattcgt aaattaagga aaattcgacc agacattcgc ttatctactg acattattgt
    27121 aggctttccc ggagagacag acaaagattt ccaggacact atggatcttg tgcatgaaat
    27181 cggttttgat acctctttca gctttatata cagcccaaga ccaggaactc ctgcagcaaa
    27241 tttacctgat gacacgccta tggagatcaa aaagcaacgt ttacagattt tacaaaacag
    27301 attattaatg aatgcagctc gttacagtga atcaatgata ggcagcaagc aaaaaattct
    27361 ggttaccgga ttttccaaga aaagttcaca gcaattatct ggtcgtactg aatgtaaccg
    27421 tgtagttaat tttgatggac cacctcattt aattggacaa tttatagatg tccaaattag
    27481 tgacgcacta cctaattcat tgcgaggaag gctgcttgaa aaggagatgc agccagccta
    27541 atgattgagc atatccagca acaattatca gtacctcgcg aattacataa ccaacgagta
    27601 gacagcgtgc ttgccaggct gctccctgag tattcacgct ctcttatctg taactggatt
    27661 aaaacaggtg ctgttaccct taataatctg ccttgcaaac ccaaagataa agcgctgggc
    27721 ggtgatttaa ttacgattaa tgttgaccat acaatagtta atacagattt taactattgt
    27781 aaacctgaga acattccaat tcagataatc tatgaagaca gtgaagtaat tgttgttaac
    27841 aagccagccg gtcttgttgt acatccgggt gcgggaaata aggaacatac actcgttaat
    27901 gcgctgcttc atcattgtcc cgagttgcaa ctacttcctc gtgctggaat tattcacaga
    27961 ttggataaag ataccactgg tctgctgata gtggctaaaa acctgattag ccatacttct
    28021 ctcatcaggc aaatgcaaaa tcgcgaaatt aagcgccact atctgacatt agttcatggt
    28081 tatgtcattt ctggtggaac aatagatacg ggttttggca ggcatcccaa aaatagacta
    28141 aagatggctg taactgactt cgggcgtcaa gctgttaccc attataatat caataagcat
    28201 tataaagact ttaccttact gaatgttagc ttaatgacag gacgcactca tcagataaga
    28261 gtgcatatga actatattaa ccacccagtt gtaggagatc aactgtatgg aggaagaatg
    28321 aagtttcctc ctaatgctga tgaggcaata ctctcagttt taaaacaatt taaaagacaa
    28381 gcactccatg cctgttccat ttcattttgt catcctgtaa aaaatacaga aataacactg
    28441 aatgcattat tacccgatga tttcgaactc ttgttaaaca ctctggatca acacgatgaa
    28501 aaatagattt gccaattggc cggctcctaa aaatattacc gctttgagta ctacccgttt
    28561 gtatggtttc agtgacggac cctatgcgag caacaacctt gggttgcatg ttggtgataa
    28621 ccaacagcat gtattcaaaa acagacaaca gctcattgaa cttattgatc tgcctgcaga
    28681 accaatctgg ctggaacaaa cacatagcac aaaatgcatc acggtagaaa ctgaaaaaac
    28741 cagagatgct gatgctgcaa ttactcgttt acctaaactc cctttagtca ttatgacagc
    28801 agattgccta cccatattgc tatgcaatcg acagggaact gaaattgccg ccattcatgc
    28861 tggatggaaa gggctgtata atggtattat tgaaaataca ctaaagaagc taaatagcaa
    28921 taggacagat ataattgcct ggataggccc tgctatctgt aataaatgtt atgaagtggg
    28981 tgaagaagtt tacttgtctt ttaccaataa atactcctta accaggaatg cgtttgagcc
    29041 tgttcatagc aaatggttag ctgatttacc tcaaattgca gaaatagtac ttaatttgga
    29101 aggggtaaaa tcagtatttc agtcaaattt gtgtactttt gagttaaaaa atgaattcta
    29161 ttcctatcgc agagaatcac aaacaggtag aataggtact ttcatctgga ttaatgagta
    29221 aaatcaggat taataatatg attacacgta taaaattatt catagcaagt ctgcttgtat
    29281 ttttcatccc ggtagccgat gcagcacaag atcttacaaa catgcccagc ttggctcctg
    29341 ttctgaaaaa tgctatgccg gctattgtta acgtggctgt tcaagggtac ttgcctaata
    29401 atatggcatc cggaaatgca gacgatgatg acggggaaaa cagtaaacag ccttccagaa
    29461 tacctgaaaa aggcagaaaa tttgaaagta tagggtctgg agttatcatt gaccctaaaa
    29521 atggcattat tataacgaat gaccatgtta ttcgtaacgc caatttaatt actgtaactc
    29581 ttcaagatgg caggagatta aaagctcgcc tgattggagg tgatagcgaa acggacttgg
    29641 ctgttttaaa aattgatgct aaaaatttga aatccctggt aattggtgat tccgataagc
    29701 tggaagtcgg agattatgtt gttgctattg gaaacccctt tggattaaac agctttggaa
    29761 atagtcaatc tgctacgttt ggaatagtga gtgccttaaa acgcagcgat ttaaatattg
    29821 aaggtgttga aaactttatt caaactgacg cagctatcaa tcctggtaat tcaggagggg
    29881 ctttggtcaa tgcaaaaggc gaattaattg gcattaatac agccattatt tcaccctatg
    29941 gaggaaatgt aggtatcggc tttgcgatcc caattaatat ggcaaaagat gtagcgcaac
    30001 aaatcattaa atttggctct attcatcgtg gtttaatggg tatatttgtt caacatttaa
    30061 caccagaact tgcccaatca atgggatatg ccgaagattt tcaaggggct ttagtatcac
    30121 aggtcaatca aaattcacct gctcaattgg ctggcctaaa atcaggcgat gtcattgtac
    30181 aaattaatga caccaaaata acgcaggcaa cacaggtaaa aacaacgatt agtctgttgc
    30241 gagctggctc tactgctaaa attaaaatct tgcgagataa taagccgctt acattagatg
    30301 tagaagtcac agacatcaaa aaacatgaac aaaaattaca atccaataat ccatttctct
    30361 acggattagc cttgcgtaat tttgaacaag agtcaccacc tcatggtaat gttgttggag
    30421 ttcaggtagt aggtgcttcg gaaaccagtg cgggctggcg agctggctta aggccaggag
    30481 atattatcat ttctgctaat aaaacaccgg tcaaagacat taaatcttta caagctgttg
    30541 cacacgagaa agggaaacag ctattagtcc aggtgctgag aggagcagga gcactctacc
    30601 tgttgatcat ttaaattttt tatccaaata atgaaataac gcatcttctt tctttaagcc
    30661 catctctgag agatgggctt aaattttgat aaattatcct gcttttgata atgctggcta
    30721 tcaactattc cgcctatcct ccaaagcaat ctcttgagtg tgggaatatc acttttcaat
    30781 atatcaacaa acttcttttt aaatacgcta ttctgctttg aaaaaataat gcaaattcaa
    30841 tttaatataa attaattata aaaaactcag gaaacagtct tgacataaac acatgaaaaa
    30901 gacaaaatca cgacctcttg tggctatgta gctcagccgg ttagagcgcg gcactcataa
    30961 tgctgaggtc ggtggttcga gtccacccat agccaccatt tccatcatat gccccctgat
    31021 agagaagact tgaagtgagc aatcaagccc tactcccgat ttttgtctct catttgtcac
    31081 ttcaagcaat aaaatagcca ctgcaatcaa atcaggccct gcccagaaat aatatttgag
    31141 atcatcttgc atcacgaaaa gaaattatag tagcatcgtt gctcatcaca tttcataaca
    31201 tggattttat tttgttcagg attaacttaa tacgatatgt ggtacttctg tttattatta
    31261 cattgaattt agattgtatt agcgttgcat tttcatctca gatcatttat ggaacacaaa
    31321 atgctgcacg agttactggg aaaaatctga aaagaattta ttttcttcag ttaggcgctt
    31381 ttagccaaaa aaataatgct cagcaattgg caagaaaaat tcaagcgaga acacaatacc
    31441 ctgtaataat tagtcattca ggcaatgctt tctcggttcg tgtggggcct atcaatacgc
    31501 aagaactgca tcaactcagc ataaaacttt cacaaaattc caatattact ccaataaaac
    31561 ataattcatc accaaagcag acaccgacta ctcagacaaa atcacaaata cttgttaagg
    31621 aaacttctcg ccctcttcca actcattctg tgactaaaga aacaattgat gggaaaagaa
    31681 gctggtatgt aactgctaaa gttggagcac aagaaacaac accagatgat cagtttacag
    31741 taaataacga cagtggcttt cccagtcctt ttgatcagga tatttatacc aatagttccg
    31801 attcctccct gctattaggc attggtgggg gatatcgatt tacaaatata attctccccg
    31861 caatctctgt tggagtatac tattcccatt tctttaatat ggacattaat ggacaagtga
    31921 ttcagtacag cttgcccgaa ttcaccaatt acaattatca atggaaagta aaatctgatt
    31981 tggtcttagc ttccgcaaaa gccaatgtca tcagttggaa aaacttaatg ccatttgtgc
    32041 aaggaggagt tggagtgagc tccaatcgag caagccaata ttacgaaagc gccttgcctg
    32101 atgtcacacc gcgcataagc cccaattttg ccaatcatac gaccactgtg tttgcttacc
    32161 agctgggcgc tggtctggat tgggagataa gaccacaatg gttattatct ttagggtatg
    32221 agtattctga tttgggcaaa gtacggtcag gttatggcac tgatagctgg aatagagaga
    32281 cattgtcctc gaaaaatttg acaagcaacg ctggaacttt aggctttacc tatttatttc
    32341 atggataacc aaaagtgagg cataggctaa aattccaaac attgagaaac cataagttat
    32401 ttgtggcttc ccaacggtaa cttgtcaaat agaactgatt taatcttcat aaaaataatg
    32461 ggtgaaaaga ccaacatgaa atactcaact cgcatcataa tcaccacaat ccttctgtta
    32521 ttcaccttga tgggttatgc tggaaaagat cctatcagct ggcgagtcag tggagtattt
    32581 cctgctcaaa gcagcgttgg tcagacttat actgtgactt atacctttac caatcaattg
    32641 ccttttcgat tggccaaggc cttgattata gaacgatcgg caagccccaa cacagaattc
    32701 aactttaatg atcaatgttc cggtcggtta ttaaattcag gagaatcctg tgaagtcgtt
    32761 gtggcgttaa acccacttgt aatcggcctt aaaacacttt agttgactat tgcaggatat
    32821 agcaaggata gagttccttt acctcaaaca agcaccttga gtacaggaca gtccttgagt
    32881 ccgatcgtta gctttgtcat ggatggtttt ccctctcaat tagcgacagg ctcagtttcc
    32941 agttacaaat ttacgtttac caataacggg gctatttcag ctaccaattt atcagttaat
    33001 gtctcgcaat cattagggac accgaattat actacgacgt gcaataacac attgactccc
    33061 aacggagggg tttgtgaagt cgatggaacg tacacagtaa ctgccgggaa tccgtcgatt
    33121 caacaagtca ttgccagtct gtcttataca ggcccatcgg gaagcccggt aacggcacaa
    33181 acattcacgc aagtagtcaa tccagcagct ccgcttgtag gcactttagt accgccaaac
    33241 tatctacctc cattgatgat tgctggcaac cagtatacag tccagttttt attcaccgcc
    33301 agtggaactg tggatattac aaataatggc acaataacct gcctggaagg tgttaataat
    33361 tgtaacggga ctataaacat caccagctcc agctgcaccc cgcaaacatt gaccaacgcg
    33421 gcctgtcaaa tgactgctac gttcacagcg cctgctgcaa ataatccccc acggacttat
    33481 gctctgaccg caacagttcc ctactctgtt ggaggaaatc ctcaagcacc agcgactgcc
    33541 atcacttccg ggacagtact tgctgcatta ccgacagaaa gaactgtgac tttaatcaat
    33601 gagtgcgatt ttgatgtgtg gttttcgctg aatggaagcc aattaggtag ttcacccaat
    33661 tgcccaacaa caccatgccc aaatggcaca agctgtaata ccagtacgaa caaatgcttt
    33721 tggaacaatc caaccccaat aaatggtata tatttattag cagcgcttcc tggcgttaac
    33781 actaacagtg tgactatccc tgttaccaat gctgacccca atattcaatg gagtggaaat
    33841 atttcggcaa gcactttgtg caatggtaca acctgtcaac aagcggcttg tggtaatgct
    33901 ggcggtacca cctcttgcgc gccaggcatt ggtttcacac aacctgccac tcaggctgaa
    33961 atcacgatga atttaaccac ctccgacagt tatgatgtgg aagtcatcaa tggttttcac
    34021 atcccgattt ccatgcaacc catttattat aaacaaggac tcactactat tattgctacc
    34081 ccgaataatt acaattgcgg agtgcccggt aacgatacgg ccgataatgg atttggagct
    34141 tgtaactgga atacagccac tgtaccagtc attgatcaag tgccggggaa tggattttac
    34201 tgggtaactg gtggtggtca aagctgttcc atcacctctg caaatccagg ttgccctttg
    34261 atgacattat gcggattaga cagtaatttc aatcaggttt gtggcaattt tttaggatat
    34321 tggtcagccg atcaagtctg cggttcaagt aatgtcccag ctgccgtaca aagttatttt
    34381 aaatgcaatc aactcctccc tacttctaca acgccttttt atccctccgg cgctgtctta
    34441 tcgaatttga tgctttgctc tgttcctaca ggatttactg gtccaagata caatacctgc
    34501 tataatgctt atccctcttc ttccgctaca gacattgccc aatgttgtgg gtgcgcagac
    34561 tggtggaatc ctgttcaaac caacaatgta gtgattggag caaatcctaa taccgaaagt
    34621 tgtactcagc ctggtgcctt gcaaccacaa accaatgccc aatggaactc ttttgtacag
    34681 ccaatgatac aatggatgaa acaagcttgc ccttctgctt atatttatcc ttttgacgac
    34741 aaaaccagtg gatttacttg tactaataac ctttcaggac aacctaactc tactagctat
    34801 actatcaggt tctgcccagg aggcatcaca ggtcttcctg caggagtgaa tgagggacgc
    34861 ggataaaata tatagctggt attaacgctt agtactttat agttgcaaga aacgagtcgt
    34921 taataccagt aaacatgaac aacaattact ttgatataat ttcctgcact tcaaagatgc
    34981 gtctgtcagc agaaaattta gtttgaaaca aatgaatacc atcctcacgc atctgaggtg
    35041 ccagctcaga caagtacctt gacaaactat ctccattgtc cagctggtat tgaacagtca
    35101 atttttcctt agctgatttt gtttcttttt cctgctttag aatgcaggct tgcataaagc
    35161 ccggaaattg aattatttcc gcaacatgtt ttttgagcca tgcttgaaat tgtacaaata
    35221 tctccggatc aatcgttagg tttacttcat aaatcaccat aatttatctt cccaaattcc
    35281 cataatttga attcaccaat agcacacaac aactgaattt atttttagaa taatgagtca
    35341 tgccatgatt agatacgatc taccatttag ctataaagaa ataataaatt gtattttaat
    35401 ttatacttta gttacttgaa ttttaaatga ccgattatct gtttgccgct gatttacaat
    35461 agtatccata aagcatactc agcatacctt gaacaagtca aagaatgtag actatttggc
    35521 aagacattta aaaatcaaat aacaatatga ataacgcaat attcccagca aatctttatt
    35581 aaaaaaagtt ccatattact ttattttaaa tgataatgat tatcattttt atttaaaata
    35641 aaaccaaatg ctgtatataa gaaattacat taggacaaga attattttag gaatattaat
    35701 gttgaatgtt gccgggttag ctttcgcaga cgctggagac tctttattta aaaatgaaaa
    35761 attgacttac gatggacatc tgttttttaa tgccgccaac tcagcaatac aaagtgaaaa
    35821 atatttactg aatcagcagc tgtccaatat caaaatggga tctgaattac aggtgactga
    35881 ttggaacaaa atgaaaggct tgctaattta taacaccttg cctaccccag tggctcctca
    35941 attttatttt gagcaatttt atgacgaact ccaaatagag tcttcaaata ttttctttgc
    36001 atttggaaaa aaatggctcg catttggaaa ttataaaagt gatttaattt ataaaccact
    36061 tacaaaagcc ttgggacaaa ccaacgaagt ggccgctatt attggatacg attccttgta
    36121 ttatgctaac gcttcttttt ttaaacccta ttctcgcata agtacatcat cacttccgct
    36181 ttattacaat ctgaatacag gagtacataa tcaatcaatg gatgcaggag tcagttattt
    36241 atactctctg gcagaaagtc aattatttca atacaacaaa ggttttggcg ggcttttatc
    36301 acaatcatta aaaacaaggg ttccaggatt tgcaacctat tttaatttaa aatataaaaa
    36361 aacaagcact tatcttacct atgttactgc aatcaccccc tttgcattag aagacatgag
    36421 ttataataat cgaggagctt caccaaaggc attgagtata caaaatagtt atgatgttaa
    36481 tattaaaaaa ttttctttta aaatcattgg tttttatgac tacacttttc aagctctggc
    36541 actcggaata ccgaaacaaa gactgggact gggattgtcc gcaacccctt ttccttatct
    36601 tggtattcaa tttcagtatt ccagagacta tagttataga aataatgcca tagcctcggg
    36661 tatcaatcat tttgtaaatg gccgtaatac aaaaatgaat aatttggcat tacaaaccat
    36721 actgaatttt taaattcagg tactcaatca aaaataaaaa actatctctc tattatttaa
    36781 gcaaataaat cacaatacat tattgcaaat gataatcatt atcatttata gtgttgtcgt
    36841 ttcttactta ttcacggaaa taatcatggc agcagtttta aataattctt ataaaataaa
    36901 taactcatta catagtttat tacttgaaaa agatcttctt gtttcacaat cagaaatgga
    36961 acatttcata tcgattgcgc accagcaatg tttagatcga tt
//
LOCUS       DACTJE010000014        35866 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.014, whole genome shotgun sequence.
ACCESSION   DACTJE010000014 DACTJE010000000
VERSION     DACTJE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 35866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35866
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.014"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            77..763
                     /gene="queC"
                     /locus_tag="I9760_000490"
     CDS             77..763
                     /gene="queC"
                     /locus_tag="I9760_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAT5042283.1"
                     /translation="MKKAVVLLSGGLDSTTCLALAKSQGFACYALSFSYGQRHSAELC
                     AATRIAKHMGAADHKIVTLDIALFGGSALTDASIEVPEFKESPEIPVTYVPARNTIFL
                     AMALGYAESIGARDIFIGASSVDYSHYPDCRPEFIESFQSLANLATKAGIEGDRFTIN
                     APLQYLSKVQTIQLGTELGVDYGLTVSCYQANEAGEACGQCDSCTFRKRGFKSAGVDD
                     PTRYQKCVHI"
     gene            806..1360
                     /locus_tag="I9760_000491"
     CDS             806..1360
                     /locus_tag="I9760_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2885 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042284.1"
                     /translation="MEFFMWDKIKEWYKNNKSYLTMLLIGSAVVGLSVTFLALFFPPV
                     LAAFASISIFGMAPLAFLTSLHLPLAVLTLGCIVTGAAFGAILGGLVAWKQSISIAKH
                     VYSYFGHKQEHQVEINEEDTYRYLMGHLEGEASESFEVEVDEEFNDYTSTDSAEDRPN
                     PLNFSTDSNHQALDGGYEMTELRI"
     gene            1670..2407
                     /locus_tag="I9760_000492"
     CDS             1670..2407
                     /locus_tag="I9760_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2884 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042285.1"
                     /translation="MTLKQFATGVFYLPAAVFSGLTNLFLGSYAKDKRGNYIQDEDGN
                     YVRNRGLIGLTLDAVKYLGTSVANFISSHQKAISVAFWSSLVVAGAAALTVAFWPAAL
                     AAVVNFSVFGVSIASVVGAGYAAQVAATAGVAAALTSTGVYLGATVVNAFNALRECIS
                     NLRSKKPSSNPEFTPVNDETFEEVRRNQFSGLSKGSEQQQTVQLSSSSSEEEEPVHTT
                     KVFAQPKDVTPEVVENPTSTVSLTGSK"
     gene            complement(2463..3032)
                     /locus_tag="I9760_000493"
     CDS             complement(2463..3032)
                     /locus_tag="I9760_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAT5042286.1"
                     /translation="MANNPRLIIGISGSSGIIYGIRLLQVLQKLPIETHLVISKAGQL
                     TRAYETNISAAELKALADVYHPCTDLTASIASGSFKTLGMIIAPCSMKTLGEIAHGIS
                     SNLLTRAADVVLKERRKLVLLPRETPLHLVHLNNMVCITQMGGIICPPVPAFYNHPQN
                     IDDLINDTVGRVLDLFDLDCGLVKRWGET"
     gene            3311..5323
                     /gene="metG"
                     /locus_tag="I9760_000494"
     CDS             3311..5323
                     /gene="metG"
                     /locus_tag="I9760_000494"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAT5042287.1"
                     /translation="MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQ
                     CISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPEN
                     QALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEV
                     CGATYSPTDLINPVSVISGVSPVEKESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKL
                     SEWFEAGLKQWDISRDAPYFGFPIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFD
                     EFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKS
                     RGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIAS
                     RCAGFINKRFDNRLSSELNDQKLYNDLLSARESVIDAFVSRDYARAIRQIMDCADKVN
                     QYIDANKPWVLAKDESKLNEVHAICTMGINLFRILITYLKPVLPMMAKASEEFLNSEP
                     LHWDSIDKPLLNHRINTFKPLMVRVEKEKIEAMLVQSKESLMTTPVKENTPVEDANLI
                     SIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRL
                     TVMVANLEPRTMRFGVSEGMVLAAGDGQGIYLLQPDAGALPGMKVK"
     gene            5323..5937
                     /locus_tag="I9760_000495"
     CDS             5323..5937
                     /locus_tag="I9760_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HAT5042288.1"
                     /translation="MASVKEIDALLPQTQCGECGYAGCLPYAEALAQGAAPINKCPPG
                     GVETVKALGKLLHVDASPYLKEAIDNTRPPSVAAIKEDECIGCTKCIKACPVDAIIGS
                     SKLMHAIITHECTGCGLCVDPCPVDCIEMVSLPAAGYDKDLARQRYNAKQMRQLRDEH
                     EKQQIYRERKKLSAHAQDHTQDVQAKQNYILEALARVKAKKTYE"
     gene            5930..6565
                     /gene="nth"
                     /locus_tag="I9760_000496"
     CDS             5930..6565
                     /gene="nth"
                     /locus_tag="I9760_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAT5042289.1"
                     /translation="MNKQKSQEIFKRFQEQNPHPTTELQYSTPFELLIAVMLSAQATD
                     VSVNKATAKLFPVANTPQALLDLGLDRLKDYIKSIGLYNSKAQNIMKTCEILIDQHEG
                     QVPGQREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRIGIAKGNTPLAVE
                     QELLKKVPREFLHDAHHWLILHGRYVCTARKPHCKTCIIEDLCEYKNKNLF"
     gene            6806..8557
                     /locus_tag="I9760_000497"
     CDS             6806..8557
                     /locus_tag="I9760_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2879 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042290.1"
                     /translation="MLQESIMKWFSSVVNGVKTIGSSLWEGSKWVWNNASLSKITTGV
                     FSYTANTTFQVLEQAIALRDAVPTLVNNPQAKKIAYGMGYILVNDVLPLVALNFANNT
                     AQNYFGEGYDEDASWYAPYSLFLSGLALTNYLVKAYTWRQGVQTVAHVAVLDSLGPSS
                     FNSNKKSLPPSICDELDCNYKRKIKGWGREPLVLMGNDALTYGVSLIPYVGEPLSQVL
                     RVYFNGRYIIRLTTPELCERHKYLAMKPESVLGFGLTFEATSMLMDKALESTVGMPPF
                     LYYRALRHLLLLMHINVAAHMELPKSDLKDKASSFDPFNVYERVNRFVADVIFSGLIK
                     RIPIDFKPDKDAEPLIPLSPALKFGTMVLNMDLEKEKKTAPGFFSKAIDKAYVLVLPP
                     VFRGANGLINDPIVFMYWPTIRSGAISAVEFVEGVGKTKTMATLAWAPKTVAAAINLK
                     FGIPKKVTRFVLMLSKEEDFWNLATAFKLWFERHNIKGEPKLITGSNLALNGVTPLQP
                     TPVSVEKEPIVSPHLLITEKDTKEVVPAKSLIPVRSQPQTGVVVTISPDSLFSTRRRR
                     VNPDQEKVAITELSMTK"
     gene            complement(8670..9734)
                     /gene="corA"
                     /locus_tag="I9760_000498"
     CDS             complement(8670..9734)
                     /gene="corA"
                     /locus_tag="I9760_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAT5042291.1"
                     /translation="MNTHKTKASAKAGLLPGTAVYIGEHPPQPTQVTIHIYDKKNYKR
                     LDSFDTRQVKEAYEAGHNIWIDISGLADIEKITKICSEYNIHPLVIEDLLNTRQRPKL
                     DIVDDYIFVVFKLLQSPPDHLSYNTEQFCLIIKQKLLLTFRESEKYNLSSIYQRLNGE
                     HSLTREYGSDYLTYLIMDYIVDDYFNFVETSAQLLETLEDQLINKPETIRLQALYTIK
                     RRTITLRKIIPPLRDIVHLLLTDDGGLINKKYNLYFRDLYDHCIRLVESIDLHREMSA
                     SMLDIYLSTINNRMNESMKILTLFASIFIPLSFIAGVYGMNFKFMPELDWRYGYPMIL
                     VTMVLLAILMLYFFKRKKLF"
     gene            10004..11293
                     /locus_tag="I9760_000499"
     CDS             10004..11293
                     /locus_tag="I9760_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HAT5042292.1"
                     /translation="MNQSLSPQQSPGFLFIAVSMITCLILLINVSFKIILLGGLVFAI
                     NSLICPLIAALFLFALRNCTFKEQRHLLNICLMTLYMFCIGVYVLVNLPAAEYMHDNP
                     AYQVIFEEIPKKFFATTIAFALSFYLPHLLFCTKNDRVLSTPKQCVLLALLGGVSFFC
                     LDFYLLFSAPHAHSFKQIFIDSFMISSLLLLIIGVVYLTFLLNDKQHKGKALIETTEL
                     TLFPIYQYLICFAVTVMLVCLACEYRIIAINKDLVLSASCIFFPITIIISSIVGELWG
                     YQANLKLALALLAAQFIFDMLLMGSVALPSPPFFNLNPFYHYIMPRRLSAASLSLFVT
                     FLGNAMLLHYLKYSKWKINRSLRILIANISVNSLLCLVDYSLLYGGIYPYEQIINLAV
                     SVWQYKLLTALIALPVILWMCKILERNYSLVVQTERN"
     gene            complement(11320..12561)
                     /locus_tag="I9760_000500"
     CDS             complement(11320..12561)
                     /locus_tag="I9760_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAT5042293.1"
                     /translation="MPSAVIILLIILIAIALRQVVRVIIPIWAIMATGAIAVLLCQQI
                     TPSRAFAAVEPDVMLYLFGVFFISQAAEESGYLAFLTDQIFYYANNGKQTLMLIVFIL
                     GLSSAVLMNDTIAIIGTPIILQLCQSHKNLIKPLLLALAFSITIGSTMSPIGNPQNLL
                     IAIKGEMASPFFEFFKHLAVPTLLNLGIVYFFILFLYRNELKEPIKKPVLPLINDRSN
                     LTLVKMSLLMMLVLTMIKILTDFLHYSEFQLNFSYIALLSSVPIWFSKHRWNYIKRLD
                     WGTLVFFASTFILMQSVWDSGFFQTGMSDSHIDVNHIVVIFIMSIALSQLISNVPLVT
                     IYLPLLMSHNHSNITLLSLAAGSTIAGNLSILGAASNIIIIQNSEKRGIRGFGFLEFI
                     LVGAPLTLLNLLVYISFLQKI"
     gene            complement(12578..13963)
                     /gene="glmU"
                     /locus_tag="I9760_000501"
     CDS             complement(12578..13963)
                     /gene="glmU"
                     /locus_tag="I9760_000501"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase"
                     /protein_id="HAT5042294.1"
                     /translation="MNLQIIILAAGQGKRMYSDTPKVLHHLAGKPLLTHVVETAQQLN
                     PNAIHVIYGHGGEQIKSSLPNLPVHWVHQAEQLGTGHAVLQAMPHIPDDAYVLVLSAD
                     VPLIQVETLQSLIECSQRQNPDHSVLALLVAELENPSGLGRIIRNNQGEIYSIVEEKD
                     ANEQVKNIKEIYSGVCCTLANNLKKWLPQLSNSNAQGEYYLTEIISLAVQNKTPITSL
                     TAKNSFEVQGINNRQQLQQLERIWQQRAANQLMEKGATLADANRFDLRGELYCGKDVY
                     IDINCIFTGKVVLGNGCKIGPNCSLTNVTLGDGCEVYANSVLEGCHIANDCHIGPFAR
                     LRSGTQLASHCKIGNFVETKKAIFDEGTKASHLSYLGDVLLGKNVNVGAGTITCNYDG
                     VNKHQTIIEDGVFIGSDTQLIAPVTVGANATIGAGSTIRRNVPPDELTLTESRQKTIY
                     GWKRPVKRERD"
     gene            14085..14969
                     /locus_tag="I9760_000502"
     CDS             14085..14969
                     /locus_tag="I9760_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2874 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042295.1"
                     /translation="MNIFLKVLKKLMKEKLSELSANLKWKSETIGYNTKLSIDQKNVI
                     DKLEESIVLFKDSGNDEADLLSINALIDTARIQIQSIRESYGEPRDKGESVTCLNNLK
                     NHSNDFLGKLKKFDFDLLNKAYTETPENIVYYHAAIYLGDEIFSPRTGIDYEIRTKKE
                     VQLAVRLQALSERINPSHNLEEQRKRALQVLQDLAQDNQNAIKKDKGFSLPGLSFWGV
                     SVVTPSDWFSSGEGRFGVEFNTAVRTIQGMTESQFERPTVKQTVVKQEETKPSDVKKT
                     EATDEEDEEHSSENTHTL"
     gene            complement(15067..16077)
                     /gene="ansA"
                     /locus_tag="I9760_000503"
     CDS             complement(15067..16077)
                     /gene="ansA"
                     /locus_tag="I9760_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAT5042296.1"
                     /translation="MKKRVLIINTGGTISSIKTSHGYEPSPGFIQKALADIPALKHQD
                     MPDYVIKEYQPLLDSSNMTVTEWNRIARDIAHEYEQFDGFVVLHGTDTMAYTASALSF
                     MLEYLGKPVIVTGSQIPLSEIRNDATDNLITSLWLCAHQPIHEVCIYFNQHLFRGNRT
                     QKISAQRFNAFDSPNYPPLASIGIDIELHQHRLLPHPKAPFHLQTITPHFIANFRLFP
                     GFASDVLEYILNQPLRGLILETYGAGNAQNNDPRFLSLLKEACGRGVIIINCTQCHQG
                     KVEMNQYATGHTLKEAGLISGHDMTPEAAHCKLLYLLSKNLEIPEIKKLIETNLCGEL
                     QI"
     gene            16430..16957
                     /locus_tag="I9760_000504"
     CDS             16430..16957
                     /locus_tag="I9760_000504"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAT5042297.1"
                     /translation="MVIDRAGYRLNVGIILVNDSDRVFWGRRSGHDAWQFPQGGLAPG
                     ETAMQAMYRELHEEVGLDKGDVEILGSTRRWLKYRLPKQYLRHGSEPLVIGQKQKWYL
                     LKLVTSEQKVRLDLSDSPEFDSWRWVDFHEPEQQVIFFKRQVYIQALKELEPLLKKER
                     RTPYGLKRKRGNQRA"
     gene            16958..19252
                     /gene="ptsP"
                     /locus_tag="I9760_000505"
     CDS             16958..19252
                     /gene="ptsP"
                     /locus_tag="I9760_000505"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAT5042298.1"
                     /translation="MLKILKRIVQDVTAASDLKEALGILVQRINTAINAEAVSVYLID
                     NKNAEYVLIATEGLNKQAEFRVRIALDCGLIGLIGRREEPINIENAPSHPDFYHNPLI
                     GEDHLNAFLGVPIIQHRKLYGVLVVQQLEQRYFDDAEESFLITLAAQLGGIIAHAEAT
                     GELAELTQPKKGIGVDKVEATYTALTGIGSVPGIGIGTAVVVYPPADIDAVPRNPVEE
                     LEDEINAFYEALETTRDDIRRLSKRMKETVAEEEHALFDVYLRILDRDSLGAEVEHVI
                     REEKISAQAALATVIKKHVQQFESMGDDYLRERASDFRDLGRRVLAELQRTQREEIVY
                     PKRTILIGEEITAAALAEVPEGQLAGVVSAKGSNNSHVAILARALGVPTVMGLRGLKV
                     ELVSRRALIVDGYYGHVYISPSKSLLAEFKQLAQEEQELNQSLVSLRDKPAETTDNYR
                     VSLQVNTGLAMDAGLSMSVGAEGVGLYRSEVPFMSRDRFPSEDEQTIIYRQTLKAFAP
                     RLVTMRTLDIGGDKILPYFPVEEENPYLGWRGIRITLDHPDVFLIQVRAMMRASEELD
                     NLRIMLPMVTTLSEVEEAVYLIEQAFEELVEEGCEIVKPKIGVMIEVPAAAYLAREMA
                     KRVDFISVGSNDLTQYLLAVDRNNARVAALYDPLHPAMLRTLLKIVEGGHAAGVEVSI
                     CGEMASDPLAVILLIAMGFDTLSMNSASLPRVKWVIRNFAIANARKILAEVLEFEHPA
                     EIRFHLQKALEEEGLGGLIRAGKS"
     gene            19249..20055
                     /locus_tag="I9760_000506"
     CDS             19249..20055
                     /locus_tag="I9760_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAT5042299.1"
                     /translation="MISGELLINGVIYIIIGAFAGIMAGALGVGGGLIVVPGLVYIFQ
                     HSQVIPEDILMHVAAGCSLAVMIFTSYASLKAHNKMDEILLPLFKKLCPGLVLGTILG
                     SIMASFIPTHLLKIIFGLFLLGVAMKMLLDVRVSHTDKFPGYWLNNLVSSSIGSISGL
                     LGVGGGVIIIPYLTYCGVPVRKIAAVSNLGTFAVGLVGTIMFMITGTLDMAKISYSTG
                     YIYWPAVLGVAIPSSLIAPFGAKLNYLLPVNYLRYGFIVILITTGIHMFL"
     gene            20073..20843
                     /locus_tag="I9760_000507"
     CDS             20073..20843
                     /locus_tag="I9760_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAT5042300.1"
                     /translation="MLTYPNINPIAFSLGPLKVHWYGLMYLIGFVGAWLLGYWRIKHY
                     KLNWNNDQLSDLIFYSALGVILGGRVGYMLFYDFQEFIHHPWVLFKIWEGGMSFHGGL
                     LGVVIAAWLFCRKYGKTFLEVGDFVAPLVPLGLAAGRLGNFINGELWGRVTDVPWGMI
                     YPHVDDQPRHPSQLYEFGLEGVALFILIWCYASKPRQQGRVSALFLMGYAICRLIAES
                     FRQPDSQLGFVAFGWLTMGQVLSIPMLLIGIWLWWAKR"
     gene            20840..21634
                     /gene="thyA"
                     /locus_tag="I9760_000508"
     CDS             20840..21634
                     /gene="thyA"
                     /locus_tag="I9760_000508"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004172055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAT5042301.1"
                     /translation="MKTYLQLLEHILQHGVEKSDRTGTGTLSVFGYQMRFDLAQGFPL
                     VTTKKLHTRSIVHELLWFLRGDTNISYLKENGVTIWDEWADNNGDLGPVYGKQWRSWP
                     TADGRTIDQLSEVVQQIKSNPDSRRLIVSAWNVGELDKMALMPCHALFQFYVANNKLS
                     CQLYQRSADVFLGVPFNIASYSLLTHMVAQQCNLDVAEFIWTGGDCHLYLNHLEQAQI
                     QLTREPLPLPSLAIKRKPASLFDYAYEDFEFLNYQSHPAIKAPIAV"
     gene            21710..21979
                     /locus_tag="I9760_000509"
     CDS             21710..21979
                     /locus_tag="I9760_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042302.1"
                     /translation="MKKIIVFVLLTVVCSAGFTSKLSKFLNKMDAEQKQRDAQEWQQD
                     MNFGDYAFRLKERYVDDRGQRCRDYEFRGRSNPYRHGYFTVCDER"
     gene            complement(22096..22488)
                     /locus_tag="I9760_000510"
     CDS             complement(22096..22488)
                     /locus_tag="I9760_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAT5042303.1"
                     /translation="MTEINKIIHKKTFDIAWGDMDALGHVNNARYFDYFQEARIDWLR
                     ELDIKMTGQTGPVVIHVACTFLKPIVYPATVTIHSKVNSLGNSSMIMDHDLYQEETLM
                     AQGVSKIVWIDYTQNKSVPLPDIIRNLV"
     gene            complement(22485..24662)
                     /locus_tag="I9760_000511"
     CDS             complement(22485..24662)
                     /locus_tag="I9760_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAT5042304.1"
                     /translation="MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGI
                     VINKVCDQQTGHSLKKITSIIDEKPLVPEDLLELCLWVGSYYQSPLSEVIPLVIPKKY
                     RLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
                     NGTQIAALLACQAINLSQQVTLPTKIPEKLSPPLTLNPEQAVAVDSIFEHIHHYQCFL
                     LQGVTGSGKTEVYLHVIAKILEQGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSN
                     LNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEHDSSLKQMEGVRYSA
                     RDTALMRAHLANIPIILGTATPSLETIHNYKQKKYNLLRLTHKAASTTPLHYQLIDLR
                     SQMVQHGLALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLT
                     LHKQAGQMICHHCGLTQRIPLFCNNCQSKELIPVGSGTQRIYEFLSHQFPDTNVLRID
                     RDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGFYNQDFR
                     AIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQE
                     AEMPPFQFLAVIRAQGKTVNKVEQFLKATKDQIQAHPLTVLGPAPAPLPRKANQHRMQ
                     LLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVDPMDLS"
     gene            complement(24663..25163)
                     /locus_tag="I9760_000512"
     CDS             complement(24663..25163)
                     /locus_tag="I9760_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl tetrahydrobiopterin synthase"
                     /protein_id="HAT5042305.1"
                     /translation="MIMKKYLTTVELQKESMKFSAGHTTIFSATEREPLHGHMYSVYL
                     ALTTWVEENGMTFDYRYYKERIHKLCRYLNQTFLMPQFSPFLEYSEDEEYYYFIFNKK
                     KIPFLKEDITLMPLTNITVEELSRWFVNELIKEKEELDNHRIEKVVVKVFSAPGQSAS
                     HEWLRS"
     gene            complement(25160..26272)
                     /locus_tag="I9760_000513"
     CDS             complement(25160..26272)
                     /locus_tag="I9760_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT5042306.1"
                     /translation="MNLKQTLINQLKLQKEIPFIDFMQQALYAPYEGYYTSGLQKLGK
                     HGDFITAPELTSLYGKTLANQFQQILPLLDSPVLFEFGAGTGKLCIDTLTHLEQLQCL
                     PESYFILEVSANLRHRQQELIQQKIPQLAGLIHWLDKWPEAPFNGVIIANEVLDAMPV
                     YRFMQSETEILESYVTLDEHDQLTEIFKPVQNQRLLAYIKNRLPPLDYPYLTEANLFL
                     DDWLLNCSHMLKKGALFIIDYGFPRHEYYHPDRNQGTLMCHYQHYSHPNPLLNPGEQD
                     ITAHVDFTHVAEAGHQAGFHIAGYTNQASFLLANGLLSLIHTLDDEQELFAAKQAIKQ
                     LTQPSEMGELFKVIALTKDLEIDLNGFLINDKRVNL"
     gene            26449..27204
                     /locus_tag="I9760_000514"
     CDS             26449..27204
                     /locus_tag="I9760_000514"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HAT5042307.1"
                     /translation="MNQTNTQEAKVALVTGGAKRIGAAIVKKLHLSGYQVAIHCRSAL
                     HEADILAKDLNRLRTNSAIVMQKELTEPDAAEEIISKVKHWAGRLDLLVNNASAFKRT
                     DCHLLNEADWQYLFAVNVQAPFLLSMAACPLLAPCKGSIINITDIHADKPLKGYSVYC
                     QTKAALEMQTRSLARELAPEIRVNAVAPGAIAWPENDNSLTAEAQQKIIEKTPLKSHG
                     KPDFIAQAVLALAENSFITGQTLKVDGGRSLTS"
     gene            27330..29240
                     /locus_tag="I9760_000515"
     CDS             27330..29240
                     /locus_tag="I9760_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042308.1"
                     /translation="MSEQYWRFKLMTEGGCNQNEATRLITVLKRKLKPFPEESINKLF
                     ENDNFCNRLSSYMAYGFGAAEEWIKKQQILSNIQPLTSEQRVDITPEKDTVSDNKPNI
                     FGAAITFGKSPVVKLLKQNAREICESILMDEPNLKQVEYIFRLLALQVQETYSGEQAE
                     KLYECIRDKKPIPSKFEEILLPIVNRIKENHTEILNESKRNHLGVTIQLNDPYSFSTK
                     NSFCIWFSNNPNSAMPKKIKDILEERAKQNAPGVTKLVYSRACLTKKENTNFVQWAKE
                     NGITLLDFDELKCQGEDLELWNLAQAELKAMREGKGGNPAAASDLVRWISGVIGDVPI
                     AYVDADMPMLTGNKSIKSEEVYAGHPVLLNMGSALVKDGVNLPMENVAFNTDIINFTG
                     ECKDRSIAIKRIAQSLIGNYLHVTERISKSGNPELKRLGLMPGYHQLLKDCEENNNKL
                     SLPMLRKALTQAHSNLSSYVRFIGVQRFAEMVGAPEDAPLFQEALQQGNTIVLTNALV
                     AYLVHGMDNVSRLNSSEKENLIKKYLGTQLSLLYKPLVMEFSGPCAVTREILPLLPTG
                     EPTRYIENLKQPDAQILRVLQTHACVAGKTNFTSDNIPNWITSSEEVERTQSLRTDGL
                     SWMPSEQARLSK"
     gene            complement(29416..30402)
                     /locus_tag="I9760_000516"
     CDS             complement(29416..30402)
                     /locus_tag="I9760_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HAT5042309.1"
                     /translation="MHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPA
                     YCVSEMCSAIDILHKHSSQSRYIYRAPTEKFLAYQISGNEPHVLAEAAAKLQSLGADL
                     IDINCGCPKSKIRKKGAGSALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLTL
                     AKQIEDAGAHALIVHGRRWTEDYDIPCNWQQIANIKNAVNIPVIVNGDINNIFSLRKA
                     LEISACDGFMISRAGTGKPWLYRDLLNLETNDVAFDEKLKLFMLHLEGLSCLENEYKA
                     VLQSKSLVRYYFKKQLNEQQLQQFYQLGSLMEIKDFLAEATHRVSPILVAAS"
     gene            30484..30927
                     /locus_tag="I9760_000517"
     CDS             30484..30927
                     /locus_tag="I9760_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042310.1"
                     /translation="MDRRYLSPLELLGIATQHAYTADYMLQQIANGMYRGGETIAVFS
                     PITSLMYVAFQLTFKAYCLHDHRPIKEYKNLMELVELNSHLGLSSNDIFLLKTLSRQQ
                     VFNKGVDYDLWENQQQLHVFCEEIISLYERVQSMMPLELQSDYQE"
     gene            31104..32099
                     /locus_tag="I9760_000518"
     CDS             31104..32099
                     /locus_tag="I9760_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAT5042311.1"
                     /translation="MEQLDDKVADNVQQKILQLSRHLNSRILGQKDLISRLLIALLAD
                     GHLLVEGAPGLAKTRAVKELSDVVEGDFHRIQFTPDLLPGDLTGTDVYHPQNGSFVFQ
                     PGPIFHHLILADEINRAPAKVQSALLEAMAERQVTIGGKTYPLPELFLVMATQNPIEQ
                     EGTYPLPEAQLDRFLMYVKISYPDAKVEHDILTLARKEALGNATENQIKVTKLSQKTL
                     FEARKQVLKVHTSEALENYLVQLVVATRNPGVYSDELGRWLRFGASPRATIALDRCSK
                     AHAWLAGRDYVTPDDIHVIAHDVLRHRILLSFEAEAEGISSDDFIDSLLRLVAIP"
     gene            32109..33020
                     /locus_tag="I9760_000519"
     CDS             32109..33020
                     /locus_tag="I9760_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAT5042312.1"
                     /translation="MTDGVVAELSELIDLRRYVHSVHYKPQGRALRSGNHISKFRGRG
                     MDFAEVRNYQAGDEIRHMEWRVTARTGRPHVKIYQEERERPVVLLVDFNPSMIFGTKI
                     AFKSVVAARLASLLAWTVIKQGDRVGGFFFSATEHSEFIPRGRDSGVLPLLASLSRYT
                     TQTEVQREEKPRMLSDALMRLRRVIRPGSILVLISDFYSMDVECEKHLNRLRGHNDIL
                     AYHVCDRIELAPPKPQQYAISDGKQEIILDTGLETVSTAYQLYCQQRIEQLNDQFKRL
                     QIQYVQVTADTNLTPLVRLTFPRRSRG"
     gene            33013..33498
                     /locus_tag="I9760_000520"
     CDS             33013..33498
                     /locus_tag="I9760_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HAT5042313.1"
                     /translation="MAKTEPLAQLRDIHLPEPVGWWPLAPGWYGLMVLIVVLVAGVAY
                     FIYKRHVNALPKKQALTLLKIHKEQYEKDKNTQLASAHISELLKRVALVYYPRAEVAS
                     MHGEAWVEFLNQTGKGIDFTPVKSMLLDSPYKTSDALNLNPLFTRAEKWIKQRGKPCS
                     N"
     gene            33486..34520
                     /locus_tag="I9760_000521"
     CDS             33486..34520
                     /locus_tag="I9760_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAT5042314.1"
                     /translation="MFELATPWALSLIPVPLFLWYLLPRAKVKLPAALKVPFFEAMAG
                     IVEQENRLISAKTLLLIPVLVWVLLVIALSGPRWVGEPKPVAREGYNIMMVLDLSGSM
                     EITDMLLHGRPVSRLLVVKRAAEQFVEDRVGDRIGLILFGTRAYLQTPLTYDRHSVLM
                     RIDDATAGLAGKTTSIGDAVGLAVKRLQDVPSKGRVIILLTDGANNSGVLAPLKAAEL
                     AKQDGIKIYTIGLGSEADPRALTGDFFAPTLSAELDEKTLEKMAKMTGGRYFRATDPE
                     SLQSIYQTINQLETVKQEQATVRPQKEYYPWFVAFALLLCFYWLISKADLFVYLRSFL
                     KVERSLSHDS"
     gene            34510..35442
                     /locus_tag="I9760_000522"
     CDS             34510..35442
                     /locus_tag="I9760_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAT5042315.1"
                     /translation="MIAEFHFLRPWCLLMIVPLFALVVIYWRQKPKLHAWEAVCDPHL
                     LEHLLHSKGQSKRALSLSFLFFSILFMILSVSGPTWYKLPQSTYKPIQPRVLLLDMSD
                     KMMLGDLSPNRLSRAKFKLHDLFTRKDIGQFGLIVFTGEPFVVSPLTDDGQTISSLLS
                     ALTPDIMPVAGYNLDSALDEASQLIKQAGYDHGEILVLTAETPSSSAIEKAKKLAESG
                     IFSSIMPIRADENLNPLFQRFANAGDGYLLVYSPDSSDLEKWLNASQGKEKFALEKNE
                     DIPLWRDEGRWFLIPALLLLLPVFQRGWMQRVTI"
     gene            35439..>35866
                     /locus_tag="I9760_000523"
     CDS             35439..>35866
                     /locus_tag="I9760_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAT5042316.1"
                     /translation="MMWRVLTIVAAALISNQLKAFSWSDLWFTPNQQAQHLMEKGQFA
                     KAKDLFERNDWAASAAYRAGDYEQAAKLFNELKTEQGYYNQGNALAHLGKYEEAIRAY
                     DKALAFNPNNQDALYNRKLISELLKKDKEKKKSQNQQDKD"
BASE COUNT        10516 a         6534 c         7784 g        11032 t
ORIGIN      
        1 gtggcataat tgacctgcat tttagcagga atttttaaga gcaagcaatt gttccaattt
       61 tttgaggttt tgcattatga aaaaagctgt ggtcttgctt tctggcggtt tggattcaac
      121 gacttgtctc gcgttggcta aatctcaggg gtttgcttgt tatgcattaa gcttttctta
      181 cggccagagg cattccgcag aactgtgtgc cgcaactcga attgcgaaac atatgggtgc
      241 agctgatcat aaaatagtca ccctggatat cgctttattt ggtggctccg ctttgactga
      301 tgcatctatt gaggtccccg aattcaagga aagcccggaa ataccagtca cctatgtccc
      361 agcccgtaat actatttttt tggctatggc tttgggttat gctgaatcaa ttggtgccag
      421 agatattttt attggtgcca gttctgttga ttattcccat tatcctgatt gtcgcccgga
      481 gtttattgaa tctttccaat ctttggcaaa cttggcaacg aaagcaggaa ttgaaggcga
      541 tcgctttact atcaatgctc ctctgcaata tctgagcaaa gtgcagacta ttcaattagg
      601 tactgaactt ggtgttgatt acggattaac ggtttcctgc tatcaagcga atgaagcagg
      661 tgaagcttgc ggacagtgtg atagctgtac ttttagaaag cggggtttta agagtgctgg
      721 agtagatgat cccactcgat atcaaaaatg tgttcatatt taacccacac agttaaatta
      781 atgatataat ccctgaaatt ttggtttgga gttttttatg tgggataaaa tcaaagaatg
      841 gtataaaaat aacaagagct atttgacaat gcttttgatt ggatcggcgg ttgtaggttt
      901 gagtgttaca tttctggctc tgttcttccc cccggttctg gcggcatttg ccagcataag
      961 tatttttgga atggcaccat tggctttttt aacgtcattg catcttcctc tcgctgtgct
     1021 aaccctgggc tgtattgtta ctggagctgc ttttggagca attttaggag gcctggtggc
     1081 atggaaacaa tcaattagca ttgctaaaca tgtttattca tactttggtc ataagcagga
     1141 acatcaagtt gagatcaatg aagaggatac ctaccgttat ttaatgggtc atttggaggg
     1201 agaggccagt gagtcttttg aagtcgaggt ggatgaagaa tttaatgatt atacttctac
     1261 tgattctgct gaagacagac caaacccatt gaatttttca acagattcaa accatcaggc
     1321 tcttgatggg ggctatgaaa tgacagaact tagaatctaa aattcgagca caaatcaaat
     1381 atatcttcag ggtttacgaa aaacgttgat cgcttgaatt tgatcattta gcttattcaa
     1441 gcatcttaat tgatggcatt ttttgcttct atcttgggtt ttcgaaaatc ctgatcacct
     1501 gatcatacat tgtaaatttt atcgagctct tggcaaaaac ctggttgaga tgaagacaat
     1561 aaaatgaact tactttacac ttttgtaaca acatattgcg cacaattaat tcacatttag
     1621 gaaatgggtt gctacaatct gctccgattt ataaccttgg agtaaaaaaa tgacattaaa
     1681 acaatttgca actggcgtat tttatttgcc agcagccgtt ttttctggtt taactaacct
     1741 atttttaggt tcttacgcaa aagacaaacg cggtaactac attcaagatg aagatggtaa
     1801 ctatgtaaga aaccgtggtt tgattggttt gactctggac gctgttaaat atttgggaac
     1861 aagcgttgcc aatttcattt ctagtcatca aaaggcaatt tcagttgcgt tttggtcttc
     1921 tttagttgtt gctggtgctg ctgctttaac cgttgctttc tggcctgctg ctttggctgc
     1981 tgtagttaac ttctctgttt ttggagtatc aatcgcttca gtagttggtg ctggttatgc
     2041 ggctcaagtt gctgctactg ccggtgttgc tgctgcttta accagtactg gcgtttatct
     2101 tggcgctaca gttgttaatg cttttaacgc attaagagaa tgcatctcta atttgcgtag
     2161 caaaaagcct tccagcaacc cagagtttac acctgttaac gatgagacat ttgaagaagt
     2221 acgtagaaac caattcagtg gcttaagcaa aggttctgag caacaacaaa ctgtacaact
     2281 ttccagttca agctctgaag aagaagagcc tgtccacact actaaggtat ttgcacaacc
     2341 taaggatgtt actcctgaag tggttgagaa cccaacttct actgtatccc tcactggctc
     2401 aaagtaatat aaaaatatta caagcaagtg gtcattaaaa acgcggcttt agccgcgttt
     2461 ttttatgttt cgccccagcg tttcaccaat ccacaatcca aatcaaataa atcgagtact
     2521 ctcccaacgg tatcattgat taagtcgtct atgttttgcg gatgattata aaaggcgggc
     2581 actggagggc agataatgcc tcccatttgt gtaatacaca ccatattgtt cagatgaaca
     2641 agatgcaaag gtgtttccct cggcaagagc accaattttc tgcgttcttt aagaacgaca
     2701 tcagcggctc gggtcaacag gttgctactg ataccatgcg caatttcacc taaggttttc
     2761 attgaacagg gcgcaatgat catgcctaaa gttttaaatg agccgctggc aatggatgcg
     2821 gtgaggtcag tgcagggatg atagacatct gctaaagctt tgagttctgc ggcactaata
     2881 tttgtttcat aagcacgggt caattgccct gctttgctaa tgactaaatg cgtttctatg
     2941 ggtagttttt gcaagacttg taatagtcta atcccataga ttatgcctga tgagccactg
     3001 atgcctatga taagtcttgg attgttggcc atgtgtattc actcgttgaa aatttaattt
     3061 gatcaggact tacgaccccc caatctcttt attaaaaaca ttttttacct tgaccttgtg
     3121 gtttctcgta gtcctgttga tattaacaaa ttataagaga ataagtagta cttaaattat
     3181 cggatcatct cggtaaaagt taacacttca taaataacta ccaataaaaa cctgttttct
     3241 attgcagcat ttccattgtc tttgcgttat aattagtcgc tttattatag gtgatgagta
     3301 ttctataact atgactagtg aacgaaaaat gttagtgacc agtgcactgc cttatgctaa
     3361 tgggcatttg catctgggac atttggttga gcatattcaa acagatattt gggtccgtac
     3421 tcacaaaatg ttgggtattc aatgtattag cgtttgtggg gatgacgctc atggaacacc
     3481 cattatgctg aaagcagagc aactgggaat tactccggaa gctttaaccg ccgaaattaa
     3541 gttaagtcat gaaaaggatt ttaaagcctt cgccattgat tatgattatt accataccac
     3601 ccattcacct gaaaatcagg cattggcgac taccatattt gaacgtttgc aggcaggcgg
     3661 tgatatagtc aagaaaacaa ttcgccagtt ttatgatccc gtaaagcaaa tgtttttgcc
     3721 ggacaggtat gtgaagggaa cttgccccaa atgtgctgct gtcgatcagt atggcgataa
     3781 ttgcgaggtt tgtggcgcca cttattcgcc gacggatttg attaaccctg tttctgttat
     3841 atcgggagtc agcccagtag aaaaagaatc agagcattat ttttttgatt tgccgcgtta
     3901 cgaagagtta cttaaggatt ggacaagaaa ggggcattta caaaccgaag taacgaataa
     3961 actcagcgaa tggtttgagg cgggtttaaa gcaatgggat atttctcgcg atgctcccta
     4021 ttttggcttc cctatccccg gtgttccgga taaatacttt tacgtttggt tagatgctcc
     4081 cataggctat atggcaagct ttaaaaagta ttgcgatgag cgtggcgttt cttttgatga
     4141 gttttgggat aaggcttcaa aaacagaatt atatcatttt gtaggcaagg atattgtgta
     4201 ttttcatgct ttgttctggc cagctatgtt agctgccagc ggtttcagaa cacctacggc
     4261 cgtgtatacc catgggtttt taacggttga ggggcagaaa atgtccaaat cgcgcggtac
     4321 ttttatagaa gcacgcgctt atctggctca cttgcatcct gaatatctgc gttactattt
     4381 cgctgccaaa ttgaatggtc gagtagacga tttggatttg aattttgacg attttgtcaa
     4441 tagggtgaat gcggatctgg tcgggaaggt agtgaatatt gcaagccgat gtgcgggatt
     4501 tattaataag cgttttgata atcggttgag ctcagagttg aatgatcaaa aattatacaa
     4561 cgacttgctg agcgcaagag agtccgtgat tgacgcattt gtttcaagag actatgcgcg
     4621 ggcaataaga caaatcatgg attgtgctga caaagtcaat caatacatcg atgccaataa
     4681 accttgggta ttggctaaag atgaaagcaa attaaatgaa gttcatgcca tatgcacaat
     4741 gggaatcaat ttattccgca ttttaataac ctatttaaaa ccagtcttgc caatgatggc
     4801 caaagcttca gaagaatttt taaattctga gcctttacac tgggattcta tcgataaacc
     4861 tttgctaaac catcgtatca atacttttaa gcctttgatg gttagagtag aaaaagaaaa
     4921 aattgaagcg atgttggttc aaagcaagga atctctgatg actaccccag taaaagaaaa
     4981 cacgccagtt gaggacgcta acttaatcag tattgaggac tttgccaaag tcgatttacg
     5041 gattgcaaaa attgtcaatg cagagcctgt ggaaggcgca gataaactaa tgcgcttgat
     5101 tttagacttg ggtgatgctc aaaagcaggt ttttgccggc ataaaaaagg cttatgatgc
     5161 tgaagaattg attgggcgtt tgacagtaat ggttgctaat ttggagcccc gcactatgcg
     5221 tttcggtgtt tctgaaggca tggtattggc ggcaggggat gggcagggaa tttatttatt
     5281 acagcctgat gcaggagcac ttcctggcat gaaggtgaag taatggcctc tgttaaagaa
     5341 attgatgcgt tattgccgca aacccagtgt ggggaatgcg gatatgcagg ttgtttgcct
     5401 tatgctgaag cgcttgccca aggagcggcc cctattaata aatgccctcc tggtggtgtt
     5461 gaaactgtaa aagctttggg aaaattactg catgttgatg catctccata tttgaaagaa
     5521 gcaatagata atactcggcc tccttcagtt gctgctatca aagaggatga atgcattggt
     5581 tgtactaaat gcatcaaggc ctgtcctgtt gatgcaatta ttggtagtag taaattaatg
     5641 catgccatta ttactcatga gtgtactgga tgcggcttgt gtgtggatcc ttgtcctgtt
     5701 gactgcattg agatggttag tttgcctgcg gctggatatg ataaggattt ggcccgccag
     5761 cgttataatg ccaaacaaat gcgccagttg cgggatgagc atgaaaaaca gcaaatatat
     5821 agggaaagaa agaaactttc agcccatgca caagatcata ctcaagacgt acaggcaaaa
     5881 caaaattata ttcttgaggc tttggctcgg gtgaaagcaa aaaaaacata tgaataaaca
     5941 aaaatcacaa gaaattttca aacgttttca agaacaaaac cctcatccta caacggagtt
     6001 gcagtatagt accccctttg agttattaat tgccgtcatg ctttcagccc aggcaacgga
     6061 cgtcagtgtg aacaaagcga cagctaaact attcccggta gcgaataccc cacaagcctt
     6121 actggatttg ggattagata gactaaaaga ttacattaag tccataggtc tttataatag
     6181 caaagcacaa aatatcatga aaacctgtga aatacttatt gatcagcatg agggacaagt
     6241 tcctggccag agagaggcgc tggaagcatt gcctggtgtt ggccgaaaaa ctgcaaatgt
     6301 ggttttgaat acagcatttg gtcagccaac catagcggtc gacacccata ttttcagagt
     6361 ggctaaccgg attggcatag cgaagggtaa tacaccgctg gcggttgagc aggagttatt
     6421 aaaaaaagtg cctcgtgagt ttttacatga cgctcatcac tggttaatat tacatggccg
     6481 ttatgtctgt acagcacgta aaccacattg caagacttgc atcattgaag atctttgcga
     6541 atacaaaaac aagaatttat tttaagacaa agccataatg aaggaaaatc aaacgataat
     6601 gaacaatcta tgcctcataa aaatgactgg ctgttgtatt tcaaattcat tctatcaaat
     6661 catccgggtt gagattagct tttgtacagt taaaacacaa aagttcaatt gaccatcagc
     6721 tcttataaaa aatttcaata aagttcaaga aaagtgctct ttccctctgg tatttcccaa
     6781 tttttactca taaaatgaaa ttccgctgtt gcaggagagc attatgaagt ggttttcatc
     6841 ggtggtaaat ggagtcaaaa caattgggtc aagcctgtgg gaaggttcaa aatgggtttg
     6901 gaacaacgcc tcattgtcaa aaatcacgac tggtgtattt agctataccg caaatacaac
     6961 ttttcaagtg ttagagcagg caatagcctt acgtgatgct gttcccacct tggtcaacaa
     7021 tcctcaggcg aagaaaattg cctatggaat gggttacatt cttgttaacg atgttttacc
     7081 gttagttgct ttaaattttg caaacaatac ggcacaaaat tatttcggag agggttatga
     7141 tgaggatgca tcatggtatg cgccttattc tcttttttta tcgggattag ctctaacgaa
     7201 ttatctggtc aaagcataca cctggcggca aggagttcaa actgtagcac atgtagcggt
     7261 tcttgactca ttaggccctt cctcatttaa ttcaaataaa aaatctcttc ctccttcaat
     7321 ctgcgatgaa ttggattgta actataagcg taaaattaaa ggttggggaa gagagcctct
     7381 tgttttaatg ggtaatgatg cactgactta cggggttagt ttaattcctt atgtaggtga
     7441 gcctctctcg caagtattaa gagtatattt caatggccgc tatattattc gtttaactac
     7501 accagagctg tgtgagcgcc ataaatattt ggccatgaaa ccagagtctg ttttaggatt
     7561 tgggttaacc tttgaagcga caagtatgtt aatggacaag gctctggaat ccactgtggg
     7621 catgcctcca tttttatatt acagggctct gcgccattta ttgctgctga tgcatattaa
     7681 tgttgctgca catatggagt tgcctaaatc tgatttgaaa gacaaagctt cgtcttttga
     7741 tccgtttaat gtttatgaac gcgtcaatcg ctttgtagcg gatgtcatat tttcaggttt
     7801 aattaaaagg atacccattg atttcaaacc ggataaagat gctgagccgt taattccatt
     7861 atctccagct ctaaaatttg gcactatggt gttgaatatg gatttggaga aggaaaagaa
     7921 aacagctccc ggatttttta gcaaagccat agataaagcg tatgttttgg ttttaccgcc
     7981 tgtttttcgc ggtgctaatg gcttaatcaa cgatccaatt gtgtttatgt actggcctac
     8041 tattcgctca ggagctattt ctgctgttga atttgtagaa ggagtgggga aaaccaaaac
     8101 gatggctact ttggcatggg caccaaaaac tgtagcagcc gccatcaatc tcaaatttgg
     8161 aatccctaaa aaagtaacac gctttgtttt aatgttaagc aaagaagaag acttctggaa
     8221 cctggctaca gcattcaaat tgtggtttga gagacacaat atcaaaggtg aaccaaaatt
     8281 aattacaggt tccaatctgg ctttgaatgg ggtgacacct cttcagccta ctcccgtatc
     8341 tgttgaaaag gaacccatag tttctcccca tctactaatt acggaaaaag atactaagga
     8401 agtagtgcca gcaaaaagcc tgattcctgt tcgatcccaa cctcagactg gagttgtagt
     8461 gacgattagc cctgatagtt tattttcaac caggagacgt cgggttaatc cagatcaaga
     8521 aaaagttgcg ataacagaac tgagcatgac taaataaatt ttagaggctt ttctgacaag
     8581 agggcaacag aagtgtgttg cccggtaaag accctaccca gttctatcga tgatattatg
     8641 caggttgtaa atggggcgct ttccgccact taaaataatt tttttcgttt aaaaaaatac
     8701 agcatgagta ttgccagaag taccatggta accagaatca tagggtaacc gtatcgccaa
     8761 tccagctcag gcataaattt aaaattcatt ccataaaccc cggcaataaa acttaaagga
     8821 ataaaaatac tggcaaacaa ggtcagaatt ttcatggatt cattcatgcg attatttata
     8881 gtagacaggt atatgtctag catacttgcc gacatttctc tatgcaaatc gatcgattct
     8941 accaggcgaa tgcaatgatc atataagtcc cggaagtaaa gattatactt tttattgatt
     9001 aaccccccat cgtctgtaag caacaaatgg acaatatctc tcaaaggagg aatgattttt
     9061 ctgagggtaa tggttcttct tttgatggtg tataacgctt gtaacctaat cgtttctggt
     9121 ttattaatta attgatcttc caaggtttca agcagttggg ctgaagtttc gacaaagttg
     9181 aaataatcat caacaatata gtccatgatc agataggtca aataatcact tccatattct
     9241 ctggttaacg agtgctctcc attaaggcgt tgataaatgg aagataaatt atatttttca
     9301 gattctctga atgtgagtaa aagtttttgc ttgatgatta aacagaactg ttctgtattg
     9361 taagataagt gatcgggagg ggattgtaat aatttaaaaa caacaaaaat ataatcgtct
     9421 acaatatcca gtttgggtct ttgtctggta ttaagcaaat cctcgataac caaggggtgt
     9481 atgttgtatt ctgagcaaat tttagtaatt ttctcgatgt ctgcaagacc tgaaatatct
     9541 atccaaatat tatgtccagc ttcataggcc tctttcactt gtcgggtatc gaaggaatct
     9601 aaacgcttat aatttttttt gtcataaata tgtatcgtta cttgagttgg ttgcggtggg
     9661 tgttcgccta tatatacggc agtccccggt aagagtcccg cttttgctga agcctttgtt
     9721 ttatgagtat tcatgaagtt atctcagttt atttattttc ttattaactt atcatttagt
     9781 catcgtttaa gtcgacagtt gacgactctc gtttatcttg ctatgcaaag ggctataatg
     9841 ctatatttgc gacctgatta gcttaataaa gggcaattat tatttgaagc ttatagggat
     9901 gttgttaata cttccatgta aatcataaag aattaacccc ctatagtcag tctctatcaa
     9961 taaggcagaa tgagttgtta tatactatgg aagctcttgt cgtatgaatc aatcgttatc
    10021 accccaacaa tcccccgggt ttctattcat cgcagtgagt atgataactt gcctgatatt
    10081 attaattaac gtttctttta aaattattct actcgggggg cttgtatttg caataaacag
    10141 cttgatttgt ccccttattg cagcgttatt tttattcgca ttgagaaatt gtaccttcaa
    10201 agagcaacgc catttactta atatttgttt gatgacttta tatatgtttt gtattggggt
    10261 ttatgtgctc gtcaatttac ctgctgctga atacatgcat gataatccag cctatcaagt
    10321 aatttttgaa gaaataccca agaaattttt tgctacaact attgcttttg cactcagttt
    10381 ctatttgcct catttattgt tttgcactaa aaatgacagg gttttatcaa ctccaaagca
    10441 atgcgtgtta ttagctttgc tggggggagt aagttttttt tgcctggatt tttatctatt
    10501 attttctgct ccacatgctc acagtttcaa gcaaattttc atcgattcat tcatgatctc
    10561 gtctcttttg ctcctaatta ttggcgtagt ttatttaaca tttttattga acgacaaaca
    10621 acataagggc aaagcattaa ttgaaaccac agaactaacg ctttttccaa tttatcaata
    10681 tttgatttgc ttcgctgtga ccgtgatgtt ggtgtgtttg gcctgtgagt atcgaatcat
    10741 agcaatcaat aaagacttgg ttttatcggc cagctgtatt ttctttccaa ttactatcat
    10801 tatcagttct atagttggcg aattgtgggg ttaccaggcc aacttgaaat tagcattggc
    10861 tcttcttgcg gcacaattta tttttgatat gttattgatg gggagtgttg cactaccttc
    10921 tcctcctttt tttaatttga atccttttta tcattacatc atgccacgca ggttatcagc
    10981 tgcatcgttg tcattgtttg tcacttttct tggtaatgca atgctattgc actatttaaa
    11041 gtattcaaaa tggaaaatta accgatcctt gcgaatatta atcgctaata tatcagtcaa
    11101 ctcgttatta tgcctggttg actacagttt gctttatggt ggtatttatc cttacgaaca
    11161 aattattaat ttagcggtga gtgtttggca atacaaacta ttgacagcgt taattgctct
    11221 cccggtcatt ttatggatgt gcaagatttt agaaagaaat tattcattag ttgtgcaaac
    11281 agagaggaat taaataaaca aatttaatgg ttcattctct tatatttttt gaaggaatga
    11341 gatatacact aatagattaa gtaaggtaag tggggctccc acaagtataa actccagaaa
    11401 cccaaaaccc ctgataccac gtttttcact attttgaata ataataatgt tacttgctgc
    11461 ccccaggata gagagattac cggcaatagt acttcccgca gccaggctca gcagggttat
    11521 attggaatga ttatgactca tcaataaagg caaatatatt gttactaatg ggacattaga
    11581 gatcaattgg ctcagggcga tactcattat aaaaatcact acgatgtggt tgacatcaat
    11641 atgagaatca ctcattccgg tttggaaaaa gccgctatcc catacgcttt gcattaaaat
    11701 aaaggtgctc gcaaaaaaaa ctaaagttcc ccaatccagt ctttttatat aattccatcg
    11761 atgcttgcta aaccaaattg gtacagacga caagagtgca atataactaa aattcagctg
    11821 aaattctgaa taatgcagga aatccgtcaa gattttgatc atagtcagaa ctaacatcat
    11881 gagcaagctc attttaacta aagtaagatt gctacggtca tttatcaaag gtaggactgg
    11941 ttttttaata ggttctttta attcatttct gtataaaaaa agaataaaaa aataaactat
    12001 acccagattt aacaaagtag gtacagccaa gtgtttgaaa aactcaaaaa atggcgaagc
    12061 catctctccc ttaatagcaa tcaataaatt ttgtggattg cctataggac tcatggtgct
    12121 tccaatggta attgaaaatg ccaacgccaa cagcaagggt ttaattaaat ttttatgtga
    12181 ttgacacagt tgtaaaatga taggtgtacc tataatggca atggtatcat tcattaacac
    12241 cgcggagctt aaacccagaa taaaaacaat caacattaac gtttgttttc cattatttgc
    12301 ataataaaaa atttgatcgg tcagaaaagc gaggtagcca ctttcctctg cggcttgtga
    12361 aatgaaaaaa acaccgaaca aataaagcat gacatccggc tcaacggcag cgaaagcgcg
    12421 tgatggtgtt atctgttggc agagcaaaac agcaatggct cctgttgcca taattgccca
    12481 aattggaatg ataactctga caacctgccg taaagcaatt gcaataagaa taatcagaag
    12541 aatgatgaca gcagaaggca tttctgtcct cttttattta atccctctct ctttttaccg
    12601 gccttttcca gccgtagatg gttttttgtc gtgattcggt taatgttaac tcatcgggcg
    12661 gcacattccg gcgtatggtg ctccctgctc ctattgttgc gttagcgccc acggtaactg
    12721 gagcaattaa ttgagtatct gacccaataa aaacaccatc ttcgataatt gtttgatgct
    12781 tattaacccc atcgtaatta caagtaatgg ttccagcgcc aacgtttaca ttttttccta
    12841 acaggacgtc ccctaagtaa ctgagatgac tggctttcgt gccttcatca aaaatcgctt
    12901 ttttagtttc aacaaaattg ccgattttac aatgggaagc taattgcgtc cctgagcgca
    12961 agcgggcaaa aggaccgatg tggcagtcgt ttgcaatgtg gcagccttcc agtacgctat
    13021 tagcgtaaac ttcgcaccca tcgcctaatg tcacattagt cagactgcag tttgggccaa
    13081 ttttacagcc attacctaaa actaccttgc ctgtaaaaat gcagttaatg tcgatataga
    13141 cgtctttgcc acaatataat tctcctctca aatcaaagcg gttggcatca gccagggtcg
    13201 cgcctttttc catgagttga ttggctgctc tttgctgcca gatgcgttcg agttgctgaa
    13261 gttgttggcg attattaatg ccctgtacct caaagctgtt cttggcggtt aatgacgtaa
    13321 tgggtgtttt attttgtaca gccaatgaga taatttcagt taaataatat tcgccttgtg
    13381 cgttactatt acttagttga ggcaaccatt tcttcagatt atttgccaaa gtacaacaca
    13441 ccccggaata aatttctttt atattcttta cttgctcatt tgcatccttt tcttctacaa
    13501 tgctataaat ttcaccttgg ttgtttctga taatacgccc aagaccgctt ggattttcca
    13561 gttctgcaac caggagtgct aaaacagaat gatctggatt ttgacgttgg ctgcattcaa
    13621 ttaatgattg aagagtttcc acttgtatca ggggaacatc ggcagataaa accagaacgt
    13681 atgcatcgtc agggatatgg ggcatggctt gcaaaacagc atggccagtc cctagttgct
    13741 ctgcctgatg cacccaatga acaggtaagt tggggagtga acttttaatt tgttcaccac
    13801 catgaccata aatgacatga attgcattgg gatttagttg ctgagcggtt tccacgacgt
    13861 gggttaacag cggtttgcca gctaggtggt gaagaacttt gggcgtgtcc gaatacattc
    13921 ttttaccttg ccctgcagct aaaataataa tttgtaaatt catgagattt gtgttttcct
    13981 aagttaaata tcataatcgg tgctgattta aacagtctgc cccaatgtta tgaatgatgt
    14041 aaagagctat atgataaata agttgcagtt aaaggagtag agcaatgaat atatttctaa
    14101 aggttttaaa gaaattaatg aaggaaaaat tgagtgagtt atccgccaat ttgaaatgga
    14161 agtcagaaac cattgggtat aataccaaat tatcaatcga tcaaaagaat gtcattgata
    14221 aactcgaaga aagtatcgtt ctttttaaag attcaggtaa tgacgaagca gatttgctgt
    14281 caatcaatgc attgatagat actgccagaa tacaaattca atccataaga gaatcctatg
    14341 gtgagccgag agataaaggg gaatctgtaa cctgtctcaa taatttgaaa aatcattcca
    14401 acgattttct tggcaaacta aagaaatttg attttgactt gttaaataaa gcttatacag
    14461 aaacacctga aaatattgtc tattaccatg ctgctatcta tctgggagat gaaattttta
    14521 gccctagaac agggatagat tacgaaatcc gcaccaaaaa agaagtgcaa ttggcagtaa
    14581 ggctgcaggc actaagcgag cgaattaatc caagccacaa tttggaagaa caaagaaaaa
    14641 gagcgttgca agtgttgcaa gatctcgctc aggacaatca aaacgctatc aagaaagata
    14701 aaggtttttc tcttcctggt ttaagctttt ggggtgtttc agtagttaca ccatcggatt
    14761 ggtttagctc aggagaaggg cggtttggcg ttgaatttaa tacggcggtt cgcaccattc
    14821 agggaatgac tgaaagccag tttgaaagac cgacagtaaa acaaacagtg gtaaagcaag
    14881 aagagaccaa accatccgat gtaaaaaaaa cagaggcaac agacgaggag gatgaagaac
    14941 attcatctga aaatactcat acattgtaat ttactttcaa tgggtgtatt ttgatttctc
    15001 tgcctcgaat gcatacgaga ggtctccaaa agatactttc tgttggagca aggagatccc
    15061 tcgttcttaa atctgcagtt caccacacaa attggtttca atcagttttt tgatttcagg
    15121 gatttccaga ttcttgctta ataaatacag caatttgcaa tgagccgcct caggtgtcat
    15181 atcatggccg ctaatcaagc ctgcttcttt taaagtatgt cctgttgcgt attgattcat
    15241 ttccactttc ccctggtggc attgggtgca attaataata ataacccccc tgccgcaagc
    15301 ctccttaagc aaacttaaaa atcttggatc attgttctgg gcattacctg ccccataggt
    15361 ttcaaggatt aaacctcgta aaggttgatt taaaatgtat tcaagaacgt cagaggcaaa
    15421 gcctgggaac aggcggaagt ttgcgataaa gtgaggagta atggtttgta gatggaacgg
    15481 tgctttgggg tgtggcaata aacggtgttg atgtaactcg atatcgatac caatcgatgc
    15541 caagggtggg tagttgggtg aatcaaaagc attgaagcgt tgggcactga ttttttgggt
    15601 gcgattgcct cgaaacaaat gttgattaaa gtagatgcat acttcatgga taggttgatg
    15661 agcacaaagc cataaggaag taattagatt atctgtcgca tcgttacgaa tctcggataa
    15721 aggaatctgt gaaccagtga ctatgacggg ttttcccaaa tactccaaca taaatgacaa
    15781 agcggaagca gtgtaagcca tggtatcagt gccatgtaat acaacaaatc cgtcaaactg
    15841 ttcgtattcg tgagcaatat ctctggcaat cctgttccat tccgtgacgg tcatattaga
    15901 ggaatcaagt agcggctgat attctttgat aacgtagtca ggcatgtctt gatgttttag
    15961 agcaggaatg tctgccaacg ctttttgaat gaatcccggt gaaggctcat aaccatgcga
    16021 tgtttttatg ctactgattg tgccgccagt attgataatt aaaactcgtt ttttcatgcg
    16081 gatttataat caatgattta ttattctttc gataagtata gcgaaaactt ggctatacca
    16141 ataacaacta atttcaagac ttgcgaaaag ccccaatctc tttggtaaaa aagtgtttaa
    16201 ttcggtcatt tagtttatag aaactccctc gttaaaaaca ttaaagttat ctgtctccaa
    16261 ggtattgaga gtcattatga tgagtaattt ttgttctata ataaatcaaa aaatgcaata
    16321 tgaaaacagg gaataaatta gtccaaataa tatttacaga tttaattgta agctcttgta
    16381 ctttgtggaa aaatcaagtt attaacggtt tattctaagc agtgacatca tggttatcga
    16441 tcgtgccgga tatcgcttga atgtaggtat tatactcgtc aacgattccg atagagtttt
    16501 ttgggggaga cgaagcgggc atgacgcttg gcaattcccg caaggtggat tggcaccagg
    16561 cgagacagcc atgcaagcaa tgtatcgtga attgcatgaa gaagtggggt tagataaagg
    16621 tgatgttgaa atattgggtt cgaccaggcg ttggctaaag tatcgacttc cgaagcaata
    16681 tttgcgtcat ggcagtgagc ctttggtgat tggccagaaa caaaaatggt atttgttaaa
    16741 gcttgtaaca agcgagcaaa aagtcagatt ggatttgagt gattctcccg aattcgatag
    16801 ctggcggtgg gtggattttc atgagcctga gcaacaagtt attttcttta agaggcaggt
    16861 ttatattcaa gcgttaaaag aattagaacc tttgttaaaa aaagaacgcc gtacccctta
    16921 tggcttaaaa cgtaaaagag gtaatcaaag agcttagatg cttaaaatac ttaaacggat
    16981 agtacaagat gttaccgctg ccagtgattt gaaagaagca cttggcattt tggtgcaacg
    17041 tattaataca gccattaatg cagaggctgt ttccgtttat cttattgata ataaaaatgc
    17101 tgaatacgta ttgatagcca cggaaggttt gaacaagcag gcagagttta gagtcagaat
    17161 tgctcttgat tgcggtctta tcgggttgat aggacgaaga gaggaaccta tcaatataga
    17221 aaatgctccc tctcatccgg atttttatca taatccatta atcggcgaag atcacctgaa
    17281 cgctttttta ggcgtcccta ttattcaaca tagaaaatta tacggtgttt tagttgtaca
    17341 acagcttgaa caacgttatt ttgatgatgc tgaagaatca tttcttatta ccttggcggc
    17401 tcaattaggc gggattattg ctcatgcaga ggcaacaggt gaattggccg agttaacaca
    17461 acctaaaaag ggtataggcg tcgacaaggt agaagcgact tatactgcac tgactggaat
    17521 aggcagtgtt cctggtatag gaattggtac tgccgtggtt gtgtacccac ctgcggatat
    17581 tgatgctgtc cctcgtaatc cggttgaaga attggaagat gaaatcaatg cgttttatga
    17641 agctttggaa acgacaagag atgatatcag gcgtttaagt aaacgcatga aagaaactgt
    17701 tgctgaggaa gagcatgctt tattcgatgt gtatttaaga attttggata gggatagctt
    17761 gggtgctgaa gtagagcatg tgattcgtga agagaaaatc agcgctcaag ctgccctggc
    17821 tacagtgatc aagaaacacg tgcagcaatt tgaaagcatg ggcgatgatt atctgcgcga
    17881 acgagccagc gatttccgtg atttgggccg aagagttctg gcggaattac aacgtaccca
    17941 aagagaagaa attgtttatc cgaagagaac cattttaata ggagaagaaa ttactgctgc
    18001 cgctctggca gaagttccag aagggcaatt ggctggtgtc gtttcagcta aaggctccaa
    18061 taactcccat gttgctattt tagcgagagc tctcggtgta cctactgtga tggggttgag
    18121 aggattaaaa gtagagttgg tatcgcgtcg ggctttgatt gttgatggct attatgggca
    18181 tgtttatatc tctccttcca aatcattatt agcggaattt aagcaattag ctcaagaaga
    18241 acaggaatta aaccaaagcc tggtaagctt gcgtgataaa ccagctgaaa ctactgataa
    18301 ctacagagtc tctttgcaag tcaataccgg cttggcaatg gatgccggtt tatcaatgag
    18361 tgttggcgct gaaggagttg gtttataccg ttcagaagta ccatttatga gtcgagatcg
    18421 attcccttca gaagatgagc agaccattat ttacagacag actctcaagg cctttgctcc
    18481 acgtttggtg actatgcgta ccctggatat tggtggagac aaaattctac cttattttcc
    18541 agttgaagag gaaaaccctt atttaggatg gcgtggaata aggataactc ttgatcatcc
    18601 agatgtcttc ctgattcagg ttcgtgccat gatgcgtgcc agtgaggaac tggataattt
    18661 acgtatcatg ttgcctatgg tcacaacact tagcgaagta gaggaagccg tttatttgat
    18721 tgaacaggca tttgaagagc tggtggaaga ggggtgcgaa atagtaaaac ccaaaattgg
    18781 tgtgatgatt gaagtgcctg ccgctgctta tctggcaaga gaaatggcga aacgagtaga
    18841 ttttatttcg gtgggtagta acgatcttac ccaatattta ttggctgtgg atagaaataa
    18901 cgcccgagtt gccgcacttt atgatccctt gcatccagcc atgttgcgta cattgctaaa
    18961 aatagtcgaa ggagggcatg cggctggtgt tgaggtgagt atttgtggcg aaatggccag
    19021 cgatccttta gcggtgattt tgttaattgc tatgggtttt gacaccttaa gcatgaattc
    19081 cgccagttta cccagggtga aatgggttat tcgcaatttt gctatcgcga atgcccgtaa
    19141 aattctggca gaagttttgg aatttgaaca tcctgctgaa atacgatttc atttgcaaaa
    19201 ggctctagaa gaggaaggat tagggggatt aatcagagca ggaaagtcat gatatccggt
    19261 gagttactga ttaatggagt gatttacatc attattggtg cttttgcagg aataatggcg
    19321 ggcgctctgg gcgttggtgg cgggcttatt gtggtaccag gactcgtcta tatttttcaa
    19381 cactcgcagg tgattccaga agatatcctt atgcatgtcg ctgcaggttg ttcactggcg
    19441 gtgatgattt ttacttctta tgcatcctta aaagcacata ataaaatgga tgagatactc
    19501 ttaccccttt ttaagaagtt atgtcctggt ttggttcttg gtactatttt aggttccata
    19561 atggcttctt ttattccaac ccatttattg aaaataatat ttggactgtt cttgcttggc
    19621 gtagctatga aaatgcttct tgatgtccga gtgagccaca ctgataaatt tccgggatat
    19681 tggcttaata atttagtcag tagttcaata ggttcgatat ctggtttatt aggggttgga
    19741 ggaggagtca tcattatccc ttatctgacc tattgtggag ttccagtcag aaagattgcc
    19801 gcagtatcca atttgggcac ttttgcagtt ggcctagtgg gtacaattat gtttatgatt
    19861 actggcacat tagacatggc aaaaatatcg tattccacag ggtatatata ttggccagca
    19921 gttttagggg tagcaattcc aagtagtttg attgcaccat ttggggcgaa attaaattat
    19981 cttttacccg taaattattt aagatatgga tttatcgtga ttttaattac caccggaatt
    20041 catatgtttt tgtgaaagga atttgaataa ccatgctcac ctatccaaat attaatccaa
    20101 ttgctttttc tttagggcca ttaaaagttc attggtatgg tttgatgtat ttaatcggtt
    20161 ttgttggcgc ctggctactg ggttattggc gcatcaaaca ttataaattg aactggaata
    20221 acgaccagtt aagtgatttg atcttttata gtgcccttgg tgttattctt gggggacgag
    20281 taggttacat gttgttttat gactttcagg agtttatcca ccacccctgg gtattattta
    20341 aaatatggga aggagggatg tctttccatg gtggtttgct tggagtggta attgccgcct
    20401 ggctcttttg ccgtaaatac ggaaaaactt tcttggaggt tggagacttt gtcgcccctc
    20461 tagttccttt aggtttagcg gcaggtcgcc tgggaaattt cattaatggg gaattatggg
    20521 ggcgtgtcac tgatgttccg tggggtatga tatatcctca tgttgatgat caaccaagac
    20581 acccctctca attgtatgaa tttggtttgg aaggggtcgc tttatttatt ttgatttggt
    20641 gttacgcatc aaagccgaga caacaaggga gagtgagcgc tttattttta atgggatacg
    20701 cgatttgcag gttaattgct gaaagcttta ggcaacctga ttcgcaattg ggttttgtgg
    20761 catttggctg gctgacaatg gggcaggttt tatccatccc tatgttatta attggaattt
    20821 ggttatggtg ggctaagcga tgaaaacata tttgcaattg ttggagcata ttttacaaca
    20881 tggggtagaa aaatccgata gaacgggcac tggaacatta tctgtatttg ggtatcaaat
    20941 gcgttttgat ttggcccagg gattcccgct tgttaccact aaaaaactgc atacgagaag
    21001 tattgttcat gaattactgt ggttcttgcg tggtgatacc aatatttctt atcttaagga
    21061 aaacggggta actatttggg atgaatgggc agataataat ggtgatttag ggcctgtcta
    21121 tgggaagcaa tggagaagtt ggccaactgc tgatggacgc accattgatc aattaagtga
    21181 agtagtgcaa caaataaaga gcaaccctga ctctcgtcgc ttgattgtaa gcgcctggaa
    21241 tgtgggtgaa ttggataaaa tggctttaat gccttgtcat gccttgtttc agttttatgt
    21301 tgcaaacaac aagctatcct gtcaattata tcaacgctca gccgatgtat ttttaggagt
    21361 tcctttcaat atcgcttctt attcgttgct tactcatatg gttgctcagc aatgtaattt
    21421 ggatgtggct gaatttatat ggaccggggg agattgtcat ttgtatctta atcatttgga
    21481 gcaagcgcaa atccaattaa caagagaacc tttgccattg ccctccttag ctattaagag
    21541 aaaaccggct tctttatttg actatgctta tgaggatttt gaatttttga attatcaatc
    21601 acatcccgca attaaagcgc ctatcgccgt ttaattatta tcagcattgg atttttattg
    21661 ccaactatgg tttaatattg cctcaattgt ttatttgagg ctcattgctg tgaaaaaaat
    21721 tatagtgttt gtattgctga ctgttgtttg tagcgcaggc tttacgagta aattgagtaa
    21781 atttctgaat aaaatggatg ctgagcaaaa acaaagagac gctcaggaat ggcaacagga
    21841 tatgaacttt ggagattatg cttttcgctt gaaagagcgc tatgtcgatg atcgcgggca
    21901 acgttgccgt gattacgaat tcagaggacg ctccaacccc tatcgacatg gttattttac
    21961 tgtgtgtgat gagcgttgat ttggctgaaa atactctttc tgtatattaa attatcgtct
    22021 gctccttacg gtcgcagctc ggattataac taaaactgag ccgtgaccgt aagagagcga
    22081 ttgtccgaat aggctctaca ctaaattcct aataatatca ggcaaaggca cggatttatt
    22141 ttgggtataa tcaatccaaa ctattttact cacaccttgt gccataagcg tttcttcctg
    22201 atacaaatca tggtccataa tcatgctgga attgccaaga ctatttacct tgctgtgtat
    22261 ggttacagta gcaggataaa cgattggttt cagaaaggta caggccacat gaatgacaac
    22321 aggacctgtt tgacctgtca ttttgatgtc aagctcccgg agccaatcaa tccgggcttc
    22381 ctgaaaatag tcaaaatagc gagcattgtt aacatgtcct aatgcgtcca tatcgcccca
    22441 ggcgatatca aaagtttttt tatgtattat tttgtttatt tcagtcatga taagtccata
    22501 ggatcaacat ccacattcca gcggatgcca ttacttaatt tattcattgt taaccactct
    22561 cttaattgcg tcaatgaact ttttagaact tttcttgaag gggatttaat taatagttgc
    22621 atcctatgct ggttagcttt tcgtggtaaa ggagctggtg caggacccag taccgtgaga
    22681 ggatgagctt gaatttgatc tttggttgct tttaggaatt gctccacctt gttgacggtt
    22741 ttgccctgag ctctgatgac cgctaaaaac tgaaaaggtg gcatttcagc ctcttgtctt
    22801 gaaattaata aagcgttcgc aaattcatca tacccatgct gaatgagtag attaagcagg
    22861 ggatgatcag gtaaataagt ttgaatcaat acttgcccgg gatgttctgc tcgccctgct
    22921 cgtccagata cttgagttaa taattgcccc aaatgttcaa tagcccggaa atcctggttg
    22981 taaaagcctg catcagcatc tacgacaacg accagggata gacgggggaa atggtgtccc
    23041 ttggctagca tttgagtgcc tatgatcagc tgagcttccc ctttattaat tttgtccaaa
    23101 tggctgttta gagaattttt tttacgaacc tcatctcggt caattcgtaa aacattcgta
    23161 tctggaaact gatggcttaa aaactcataa attctctggg tgccagatcc tacggggatt
    23221 aattccttac tttggcagtt attgcaaaac aagggtattc tttgggttaa tccacaatga
    23281 tgacaaatca tttgtcctgc ctgtttatgc aaggttaaat ggctatcgca tgctttgcaa
    23341 tcaaccatcc atccacattg atggcaaagt aacactgggg caaagccgcg tcggttaatg
    23401 aaaacaagaa cctggttttg ttgcagcaaa tgattttcta tgattttaag ggttgggaga
    23461 gccaagccat gctgtaccat ttgcgatcgt aaatcaatca gttgatagtg taacggtgta
    23521 gtggaggcag ctttatgcgt taagcgtaat aaattatatt ttttttgttt gtagttgtgg
    23581 atggtttcca gactgggagt ggctgtacca agaatgatag gaatattggc aagatgagca
    23641 cgcattaaag cagtatctcg cgcagaataa cgcacccctt ccatttgttt gagcgaactg
    23701 tcatgctctt catcgatgat aattaaacca agattgggta gaggggtaaa aacggcagaa
    23761 cgagttccaa tcaccatttt aactttgttc tcttttgcca gctgccaggc aatttgcctt
    23821 tctgattcat tcaaattgga atgtaatacg gttatggggt ggttaaatcg ggctgtaaaa
    23881 cgagataaaa gctgaggggt taacccgatt tctggtacca gcaccaatac ttgtttgcct
    23941 tgctccaata ttttggcaat gacatgcaaa taaacctctg tcttaccact cccggtaaca
    24001 ccttgcagta aaaagcattg gtaatgatgg atgtgttcga aaatggaatc tacggcaacg
    24061 gcttgttcag gattgagtgt tagcggtgga ctgagttttt caggaatttt ggttggcaag
    24121 gtaacttgct gtgacaggtt aattgcttga caagcaagta gtgccgcaat ttgagttccg
    24181 ttgtagccat gttgttttag ggtctgtttg gaaatccctt tctggtgcct gcttaaaaaa
    24241 tcaatcaact catgttgttt ttttgctttt ttcgagatga gtgtcttcgc cttttcaaca
    24301 ggtatattca attgataaaa atcttctgta ggtagtgagc agggtaaacc caggcgatat
    24361 ttttttggta ttaccaaagg aatcacttca gataatggtg actgataata gcttcctacc
    24421 catagacaaa gctctaagag atcttcagga acgagaggtt tctcatcgat tattgaagtg
    24481 attttcttta acgaatggcc cgtttgctgg tcacaaactt tattgattac aatgcccact
    24541 cgggcttgat tgcgaaaagg aacccatact cttccgccaa tgcatggagt taaatccagg
    24601 gcttcgtaat caaaacaatc tcgattggta tgggggatac aaacctgata aatatcgttc
    24661 atttaacttc taagccattc atgactcgct gattggccgg gcgcggaaaa cactttgact
    24721 actacttttt cgatacgatg attatctaac tcctcttttt ctttaatcag ttcattaaca
    24781 aaccagcgtg ataactcttc cacggtaata ttggttaagg gcattaaggt gatatcttct
    24841 ttaagaaaag ggattttttt cttattgaag atgaaataat aatattcttc atcttccgag
    24901 tattctaaaa aaggtgaaaa ttgtggcata agaaaagttt gatttaagta gcggcataat
    24961 ttatgaatcc gctctttgta ataacggtaa tcaaaagtca taccattttc ttctacccag
    25021 gtagttagag ccaaataaac agaatacata tgaccatgca aaggctctct ttctgttgcc
    25081 gaaaagatgg tggtatgccc ggcagaaaac ttcatggatt ctttctgtaa ttccacagta
    25141 gtcagatatt ttttcataat tataaattca ctctcttatc atttatcaga aaaccattta
    25201 aatcaatttc cagatctttg gttaacgcaa tgactttgaa tagctctccc atttcactcg
    25261 gttgagttaa ctgtttaatg gcctgttttg ctgcaaacag ttcctgttca tcatcaagag
    25321 tatggattaa gcttagcaaa ccgttcgcca gcaaaaaaga ggcttgatta gtataccctg
    25381 caatatgaaa tcccgcctgg tgccctgcct cagcaacatg agtaaaatca acatgtgcgg
    25441 tgatatcttg ttctcccggg ttaagtaaag gattgggatg agaataatgt tgataatgac
    25501 acattaacgt gccttgatta cgatcaggat gatagtattc atggcgaggg aagccataat
    25561 caataataaa caaagcccct tttttcaaca tatgactgca atttaacagc caatcatcaa
    25621 gaaacaaatt agcctcggta aggtaagggt aatccagtgg tggtaaacga tttttgatat
    25681 aggccaatag tcgttgattt tgtactggtt tgaagatttc tgttaattga tcatgttcat
    25741 ccagagtgac atagctttcc aggatttctg tttcagattg cataaagcga taaactggca
    25801 tggcatccag tacttcattc gctattatca ctccattaaa aggagcctct ggccatttgt
    25861 ccagccagtg aatcaaaccg gccaattgcg ggattttttg ttgaatcaat tcttgctgac
    25921 gatggcgtaa gttagcactt acttccagga tgaaatacga ttcaggcaag cattgtaatt
    25981 gttccaaatg agttaatgta tcaatacaga gctttccagt tccggcccca aactcgaata
    26041 gtacggggga gtccagcaag ggaagaattt gttggaattg gttggctaaa gttttgccat
    26101 acaaggaggt taattcagga gcagtgataa aatcaccgtg ttttcctagt ttttgcagtc
    26161 ctgaagtata atagccttca tagggggcat agagtgcttg ctgcataaag tctataaaag
    26221 ggatttcttt ttgcagcttt aactggttta ttagagtttg ttttaaattc ataatcactt
    26281 aaactatcgt tagatttctt ttaaactcgc tgtgaacaag agggtgcgag gagaaacaaa
    26341 gaaaatactg aagtgtacac aataatactt gaggattcga gcatcctggc tgattacttt
    26401 ttgcagtgag gttgcgaaag gagtctgtta ccatatgggg aacgtacctt gaatcagaca
    26461 aacacgcaag aagccaaggt tgctttagtg actggaggcg ctaaacgtat cggcgcagca
    26521 attgttaaaa aattacatct ctctggatat caggtagcca tccattgtcg ttctgctttg
    26581 catgaagccg atattctggc caaagatttg aatcggttgc gtacgaatag cgccattgtc
    26641 atgcaaaaag aattaactga gcctgatgca gcagaagaga tcatatccaa agtgaaacat
    26701 tgggccggtc gtttggattt gctagtgaat aatgcttctg cttttaaacg aacggattgc
    26761 catttattga atgaggcaga ctggcaatat ctgtttgcag tgaatgtcca ggctcccttt
    26821 ctactgagta tggcagcttg tcctttactg gctccatgca agggttccat tattaacata
    26881 actgatattc atgcagataa gccacttaaa ggatattcgg tatattgtca aactaaagcc
    26941 gcattggaga tgcaaacgag gtcgcttgct cgtgagctag caccagaaat aagagtcaat
    27001 gccgttgcgc ctggcgccat tgcctggcct gagaatgaca atagtttaac tgcggaagca
    27061 caacaaaaaa ttatcgagaa aacaccgtta aaaagccatg gcaaacccga ttttattgct
    27121 caggctgttc ttgctctggc tgaaaattca tttatcactg gtcaaacatt gaaagtagat
    27181 ggaggtagaa gtttgacaag ctaaaatttt tcgtaaatcc tgaatcattg gatctactgc
    27241 attttaggtt attaactgtt ctttgtgatt tgttttaacc cactttactg cttataatat
    27301 aaccaaattg gcgtctattt ggagatatta tgagcgaaca atattggcgt ttcaaattga
    27361 tgactgaggg tggatgtaat caaaatgaag caaccagact tataaccgtt ttaaaacgta
    27421 agttgaagcc attccctgag gagtcaatca ataaattatt cgaaaacgat aatttttgta
    27481 atcgactttc ctcttacatg gcatatggat ttggggcagc agaagaatgg ataaaaaagc
    27541 aacaaatatt aagtaatatc caaccgttaa cttctgaaca aagagttgat atcacaccag
    27601 agaaggatac ggttagtgat aataagccga atatctttgg cgctgcaatt acatttggaa
    27661 aatcacctgt agtgaagctt cttaagcaaa atgccagaga aatatgcgaa tcaattttaa
    27721 tggatgaacc aaatctaaaa caggtcgaat acatcttcag gttattggca cttcaagtcc
    27781 aagagactta ttcaggtgag caagctgaaa agttatacga atgtattcgg gataaaaagc
    27841 caataccctc aaaatttgag gaaatattgc tcccaatagt gaatcgtatt aaagagaacc
    27901 atacagaaat ccttaatgaa tctaaaagga atcacttggg agtaactatt cagttaaacg
    27961 atccctatag tttttcaaca aaaaattctt tttgtatttg gtttagtaat aatcctaaca
    28021 gtgccatgcc aaaaaaaatt aaggatattt tggaagaaag agccaaacaa aatgcaccag
    28081 gagtaacaaa attagtttat agccgagctt gtttgaccaa gaaagaaaat actaattttg
    28141 ttcagtgggc aaaagagaat ggtattacat tattagattt tgatgagtta aaatgtcagg
    28201 gcgaggattt agaattatgg aatttggcac aggccgagtt aaaggcgatg cgtgaaggta
    28261 aaggagggaa tcctgctgct gcctctgatc tggtgcgatg gatatcaggt gtcataggag
    28321 atgttcccat agcctacgtt gatgccgata tgccaatgct tactggtaat aaaagcatta
    28381 agtctgaaga ggtttatgcg ggacatcctg tgctattaaa tatgggatct gcattggtta
    28441 aagatggggt taatttgccc atggaaaatg ttgcatttaa tacggatatt atcaatttca
    28501 ctggtgaatg taaggataga agcattgcca ttaaacggat tgctcaatcg ctgattggca
    28561 actacctcca tgttacagag cggatttcta agagtggtaa tccagaactc aagagacttg
    28621 gcctgatgcc aggctaccat cagcttttga aagattgtga agaaaacaac aacaagttgt
    28681 ctctaccgat gttacgaaaa gcattgacac aggctcacag taatttgagt tcttatgtaa
    28741 gatttattgg cgtacagcga tttgctgaaa tggttggtgc gccagaggat gccccattat
    28801 tccaggaagc attacaacaa ggtaatacca ttgtcttaac aaatgcatta gtggcttatt
    28861 tagttcatgg tatggataac gtatcaagat taaactcatc tgaaaaagag aatttaatta
    28921 agaaatattt gggcactcag ctttctttat tatacaaacc cttggtgatg gagttctctg
    28981 ggccctgtgc agtaactcgt gaaatactac cgctattacc tactggtgaa ccaacacgtt
    29041 atattgaaaa cctcaaacag cctgatgcac aaattttacg tgtattgcaa acgcatgcct
    29101 gtgtggctgg gaagactaat tttacttccg ataatattcc aaattggata acttcctcag
    29161 aagaagttga gcgtacacaa tccctgcgaa cggatggctt gagttggatg cctagtgagc
    29221 aagcacgatt atcaaaataa gtttaattga ataagtataa cttctattaa aaaactcata
    29281 atccttggat tagatagtct tgtaatccaa ggaagttctc ctcataaccc tttaatcttc
    29341 agcgtgagct gaactaaaca gcggaatgta atggttatca aattatagaa ataatataca
    29401 aatcctgacc ttatatcatg aggcggcaac taatatgggg gaaactcggt gagttgcctc
    29461 agctaaaaaa tccttgattt ccattaatga acctaattga tagaactgtt gcagttgttg
    29521 ttcgttcagt tgctttttga agtaatagcg aaccagggac ttgctttgca gtaccgcttt
    29581 atactcgttt tcaaggcatg acaatccttc gaggtggagc ataaacagtt ttaatttctc
    29641 atcaaaagcg acatcatttg tttccagatt caataaatcc cgatatagcc agggtttgcc
    29701 cgtacctgct cgagaaatca taaaaccatc gcacgcgctg atttccaggg cctttcttaa
    29761 actgaatata ttgttaatat caccatttac aatgactggt atattgacag cattcttgat
    29821 atttgcaatt tgctgccaat tgcatgggat atcataatct tctgtccatc gcctgccatg
    29881 cactatcagg gcatgagcac cagcgtcctc tatttgtttg gctaaggtta aatccttttc
    29941 attgttttga atgcgtattt taatagtcag cggaatgctg atcgctgcgc gtaccctaga
    30001 gataatggca atcaattttt gtggatcttc caataaagcg ctaccagccc cttttttacg
    30061 aattttgctt ttagggcagc cgcaattaat atcaattaga tccgcaccca gtgattgcag
    30121 tttagctgct gcttctgcaa ggacatgtgg ctcatttccg gagatttggt aagccaggaa
    30181 tttttctgtg ggagctctgt aaatataccg ggattgagag gaatgtttat gaagaatgtc
    30241 gatggcagaa cacatttcag aaacgcaata agcaggcggt gtataaatat tgaatagatc
    30301 tctgaaaggg gcgcaactgt atcctgccaa aggtccttga atcagtcgat gagggagttt
    30361 taaagagccg atttgcagag gactgttaat aaaagtgtgc atcaatggaa ttaaaggcta
    30421 taatcgcttt ttattttaac atgattcatc atgaagaacc agaaaaaagg gagaggcttc
    30481 catatggaca ggaggtattt gtcacctttg gagctgctag gcattgctac gcaacacgcc
    30541 tatacagcag attatatgtt gcagcaaatt gccaatggaa tgtatcgagg tggggaaacc
    30601 atcgcagttt tttctccaat cacttcactc atgtacgttg cttttcagct aaccttcaaa
    30661 gcttattgtt tgcatgatca tagaccaata aaggaatata aaaatctgat ggaattggtt
    30721 gagttgaata gtcatcttgg tttatccagc aatgatattt ttcttttaaa aactttatca
    30781 agacagcaag tgtttaataa aggagtagat tacgaccttt gggaaaatca gcaacaattg
    30841 catgtttttt gtgaggaaat tatttcattg tatgagagag tgcagtcgat gatgcctctg
    30901 gagttgcaaa gtgactatca ggagtaaccg gctgaattag cgccctgtta agtgtctttg
    30961 cttaagtttc cttatgttgt gcatagccca agatgcatta atcagatatc aaaatgattt
    31021 atttttatcc aaaatagact cattctactt ggcatcccaa tggattcaag ttaggatatt
    31081 atttttttaa aagggtaatt gttatggagc aactagacga taaagtcgca gataatgtgc
    31141 aacaaaaaat actacagtta agtcgtcatc ttaattcgcg tattttgggt caaaaggatt
    31201 tgatctctcg tctgttgatc gctttgcttg cagatggcca tttgttggtt gaaggagctc
    31261 cgggtttagc aaaaaccaga gccgttaaag aattgtcaga cgtagttgaa ggtgattttc
    31321 atcggattca attcaccccc gatctgctac ctggggactt aactggtacg gacgtctacc
    31381 atccgcaaaa tggttctttt gtctttcagc ccggccctat atttcatcat cttatcctgg
    31441 ctgatgagat caaccgcgcc cctgccaaag tacagtcagc cctgcttgaa gcgatggcag
    31501 aaaggcaagt cactattgga ggaaaaactt atcctctgcc agaattgttt ctggtcatgg
    31561 caactcaaaa tcctatagaa caagaaggaa cttatccttt accagaagcc caattggaca
    31621 gatttttaat gtatgtgaaa attagttacc ctgatgcaaa agtagaacat gacattttaa
    31681 ctttggcacg taaagaagca ctgggtaatg caacagaaaa tcaaatcaaa gtgacgaaat
    31741 tgtcccaaaa aactttattt gaagctcgaa aacaagtatt gaaagtacat accagtgaag
    31801 cacttgaaaa ttatctggtt cagttggtag ttgctacccg taatcccgga gtatacagtg
    31861 acgaattggg acgctggttg cgttttgggg caagcccacg cgctactatc gcactggacc
    31921 gttgttccaa ggctcatgcc tggcttgcag gtagagatta tgtgactccc gatgatatac
    31981 acgttatcgc tcatgatgta ctgcgccaca ggattttgtt aagttttgaa gcagaagcag
    32041 aaggcattag cagcgatgac tttatagact ctttattacg tttagtcgcc atcccttaga
    32101 ggttttttat gactgacgga gttgtagctg aattaagcga attaatcgat ttaagacggt
    32161 atgttcattc cgttcattat aagccgcaag gaagagcgtt gcgttctgga aaccatattt
    32221 ccaagtttcg tggacgggga atggattttg cggaagtaag gaattatcag gctggtgatg
    32281 aaattcgtca tatggaatgg cgcgttactg cgcgtactgg aaggcctcat gtcaaaattt
    32341 atcaggagga gcgggaaagg ccagtggttc tgcttgtaga ttttaaccct tccatgattt
    32401 tcggtaccaa gatagcattt aagtcagtgg tagctgcacg attggcttct cttttagcat
    32461 ggacagtgat aaaacaagga gatcgggtag gaggattctt tttttcagct acagaacaca
    32521 gtgagtttat tccgcgagga cgagattcgg gcgtactccc tttattggct tctttaagtc
    32581 gatataccac tcaaacagag gttcagcgag aagaaaagcc acgtatgtta agtgatgcct
    32641 taatgaggct gcgtcgggtt attcggcctg gaagtatact tgttttgata agcgattttt
    32701 attctatgga tgttgagtgt gagaagcatt taaatcgttt acgaggccat aatgacatac
    32761 tggcctatca tgtttgtgac agaattgaac tggctcctcc aaaaccgcaa caatatgcca
    32821 ttagcgatgg taaacaggaa atcatattag acactggcct ggaaactgtt agcacagctt
    32881 atcagctcta ttgccagcag agaattgagc agttaaatga tcaatttaag cggttgcaaa
    32941 ttcagtatgt ccaggtgact gctgatacca atctcactcc actggtgcgc ttgacctttc
    33001 cccggaggtc tcgtggctaa aacagaacca ttagctcaat tacgagacat tcatttgcct
    33061 gagcctgttg gctggtggcc tcttgcgcca gggtggtatg gcttaatggt tcttatcgtt
    33121 gttttagtgg caggggttgc ctatttcatt tataaaaggc atgttaacgc attacccaag
    33181 aaacaagcct taactttatt aaaaatccac aaagaacagt atgaaaaaga taaaaacact
    33241 caattggcca gtgcccatat ttctgaattg ctaaagcgag ttgccttggt ttattatcca
    33301 agagcagaag tcgctagtat gcatggtgaa gcttgggttg aatttttaaa tcagactggc
    33361 aaggggattg attttactcc ggttaaatcg atgttattgg attcacccta taaaacatca
    33421 gatgcactga atttaaatcc tttatttact cgtgcagaaa aatggatcaa gcaaaggggg
    33481 aagccgtgtt cgaattagct accccatggg ctttgtcact tattcctgtc cctttgtttc
    33541 tctggtattt gttaccgcgg gcaaaagtaa aattacctgc tgcattgaag gttccttttt
    33601 ttgaggcaat ggcaggtatt gttgaacaag agaaccggct aatctcggct aaaacgctgc
    33661 tattaattcc tgttttagtt tgggtattgc tggttatcgc cctttctggt ccgcgatggg
    33721 ttggtgagcc aaagcctgta gcaagggaag gatataatat catgatggta ttggatcttt
    33781 caggcagcat ggaaattacg gatatgctac tgcatggccg accagtcagt cgtttgcttg
    33841 tcgtgaaaag ggctgcagag caatttgttg aggacagagt cggagataga atcggtttga
    33901 ttctgtttgg tactcgagct tatttgcaaa caccattaac ctatgacaga catagcgtac
    33961 taatgaggat agacgacgca acagctggtc ttgcaggtaa aactacttcg attggtgatg
    34021 cagtaggttt agccgtcaaa aggttacaag atgttccttc caaaggaaga gttattattt
    34081 tgctgacgga cggagcaaac aactcaggtg tattagcccc cttaaaagca gcagagttag
    34141 ccaagcaaga tggaatcaaa atctatacta taggcttagg ttcggaggca gatccaagag
    34201 cgttaactgg tgattttttt gcccccaccc tttccgccga actggatgaa aagacattgg
    34261 aaaaaatggc aaaaatgaca ggtggtcgtt atttcagggc gacagatcct gagtctttac
    34321 aatccatata ccaaaccatc aatcaactgg aaaccgtgaa gcaagaacag gcaactgttc
    34381 ggcctcaaaa ggaatattat ccctggttcg ttgcctttgc tttgttatta tgcttttatt
    34441 ggttgattag caaggcggat ttatttgtgt acttaaggtc ttttttaaaa gtagagcgga
    34501 gcttaagtca tgatagctga gtttcatttt ttaaggccat ggtgtttgtt gatgatagtg
    34561 cctttgtttg cgttggtggt aatttattgg cggcaaaaac caaaattaca tgcctgggag
    34621 gcggtatgtg atccccatct tctggaacat ttattacaca gtaaaggtca gagtaaacgt
    34681 gccttatctt tatcattcct gttttttagt attttattta tgattttgag tgtgtctggt
    34741 ccaacttggt acaaattgcc tcaatctact tataagccaa ttcagccaag ggttttgtta
    34801 ttggatatgt cagataaaat gatgcttggg gatttatcgc caaatcgtct tagtcgggct
    34861 aaattcaaat tgcatgattt atttacccgc aaggatatag ggcagtttgg tttgattgtt
    34921 ttcacaggcg aaccttttgt tgtatctcct ttaacagacg atggtcaaac tatctcttcc
    34981 ttattgtcag ccttgacccc tgatatcatg cccgttgcag gatataattt agacagtgcc
    35041 ttggatgaag caagccaatt aattaagcaa gcgggttatg accatggcga aattttagtt
    35101 ttaaccgctg aaacaccgtc ctctagcgct attgaaaaag ctaaaaagct ggcagaatca
    35161 ggtatttttt cttcaattat gccaatcaga gctgatgaaa atttgaaccc cttgtttcag
    35221 cgcttcgcca atgcgggaga tggatacttg ttggtatact caccagattc aagtgatttg
    35281 gaaaagtggt tgaacgcctc tcaaggcaaa gaaaaatttg ctcttgaaaa aaatgaagat
    35341 atcccattat ggcgtgatga aggccgctgg ttcttgattc ccgcattgct tttattatta
    35401 cccgtatttc agcgaggttg gatgcaaagg gtgacgatat gatgtggcgg gttttaacta
    35461 ttgtggcagc tgcactaata tccaatcaac ttaaggcatt tagttggtct gatttatggt
    35521 ttactcccaa tcaacaagct caacatttga tggaaaaggg acaatttgca aaagctaaag
    35581 atctttttga gcgcaatgac tgggctgcaa gcgcagcata cagagcagga gattatgaac
    35641 aggcagccaa attattcaat gagttaaaaa cggaacaagg ctactataat cagggaaatg
    35701 ctctagcaca cttagggaaa tatgaggaag caatacgagc atatgacaaa gccttggcat
    35761 ttaatccaaa taatcaggat gctttatata atcgaaaatt aatcagtgaa ttgttgaaga
    35821 aagataaaga aaagaaaaaa agccagaatc agcaggataa ggatca
//
LOCUS       DACTJE010000015        34467 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.015, whole genome shotgun sequence.
ACCESSION   DACTJE010000015 DACTJE010000000
VERSION     DACTJE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 34467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34467
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.015"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            14..292
                     /locus_tag="I9760_000524"
     CDS             14..292
                     /locus_tag="I9760_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018300271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5042317.1"
                     /translation="MIKLSDILHLMGEVNRLKLLIECLKGPKSVSDLAEQLQLSVPLT
                     SHHLSLLRSARLLMANREGKHIYYSIYDAHVRCILEDMLKHFTEETEN"
     gene            425..2566
                     /gene="cadA"
                     /locus_tag="I9760_000525"
     CDS             425..2566
                     /gene="cadA"
                     /locus_tag="I9760_000525"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAT5042318.1"
                     /translation="MTAPLTLTTFFVAGLDCQAEEQLIRRQLQAIPEVEKMDFNFIAE
                     EVTVHHRLPTIEPIQQQIEALGMSVRAKQSTPSDKVQEKLKFDASWQILALSGLLALC
                     SEIAAYLLATEQSLWAIIPALLAIILSGPPTFKKGWLALRTKAMNINSLMLIAITGAI
                     FIGDWPEAAMVTVLFALAERIERYSLDKARLAIRSLMQIVPDVAQVKTANGQWQTLLV
                     EEVPLGAVFRVKPGERIPLDGEVVSGQSTVNQAPITGESMPVSKQIGDSVFAGSLNEH
                     GAFEAKVSKASGDTLLAKIGKAIEQAQAERAPTQRFVDEFARYYTPIMVLIAVLVALI
                     PPFVLGYSFYDWIYKALTLLVIACPCALVISTPVTVVSGLAASAKHGLLIKGGSYLET
                     GYRLQLIALDKTGTLTEGKPVVTDFITYDKSQTNEHLLLLAASLDSHSEHPVAHALVA
                     YWQQEHPDKSLLSVERFSALPGRGVKGAIQGELYYVGNHQLAEDNKVCCLEVEQELKR
                     LEEEGKTTVILSNSTTALAIFAVADTLRANSREAIESLHRQRIKTAMLTGDNALTAQA
                     IAKQVGIDEIQANILPTEKMQAINKLLEQYQAVGMVGDGINDAPALAKATISFAMGKG
                     TDTALETADVVLMNDNLAMLPLYIDLSRKTVRILWQNISLSLVIKTVFFILALGGMAT
                     LWMAVFADMGASLIVVMNGLRLLNSNKTKNH"
     gene            complement(3105..3680)
                     /locus_tag="I9760_000526"
     CDS             complement(3105..3680)
                     /locus_tag="I9760_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAT5042319.1"
                     /translation="MKPPKKVKNSERRAREYLTPAEVDKLIASAKQLGRHGLRDSTMI
                     LIAYRHELRVSELISLRWSQIDLKQGLLHVIRKKNGIPASHPLFGPEIRALRQLKRDY
                     PETDYVFMTERKAPITADTFRKIIARAGEKAELGLATYPHMLRHSTGFKLANDGRDTR
                     SIQHYLGHKNIQHTVRYTEIASVKFKEFWDD"
     gene            3860..6878
                     /locus_tag="I9760_000527"
                     /pseudo
     CDS             3860..6878
                     /locus_tag="I9760_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            7171..7467
                     /locus_tag="I9760_000528"
     CDS             7171..7467
                     /locus_tag="I9760_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HAT5042320.1"
                     /translation="MSPAFGHHPKSPKPQKEKGFWSSPSGLMAIIIIGIIGYYLIVEH
                     GAHIASFLGASPLVLLVLLCPLMHLFMHRGHGGHGENHHHKSDDEDNDSSENKD"
     gene            7474..8139
                     /locus_tag="I9760_000529"
     CDS             7474..8139
                     /locus_tag="I9760_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HAT5042321.1"
                     /translation="MEHTSYAYGLWLLVIVNSAIFILFAYSFTTTFKTRRDWRAFGAF
                     SAFVIAYFTEMYGFPLTIYLLSGWLSKYYPGMDLYGHDSGHLLHTLLGLKGDPHFDVF
                     HILSFVFIIGGLWMIASAWGTLYRAQKAHQLATSGLYAKIRHPQYDGFILVMIGFLLQ
                     WPTILTLIMFPILVYMYVRLARREEKEVLAEFGEEYKHYAAVTPGFIPRISKQQDSKG
                     GAP"
     gene            8139..8636
                     /locus_tag="I9760_000530"
     CDS             8139..8636
                     /locus_tag="I9760_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042322.1"
                     /translation="MFMFHSAEMLGLIAIALGIMLVVWALRNEGKSIGLAKIFGWLIV
                     IIGILGELCSTYYAIKYWYAGYFQMPMMSGEAFLFHVAQMLSLIAIALGIILIAWASH
                     NEGRGISLAKVFGWFIVLFAILGMTCSAYYATVYWYKGYIQTPAGMPMLLQHQIPMQK
                     QSPNQ"
     gene            complement(8704..9525)
                     /locus_tag="I9760_000531"
     CDS             complement(8704..9525)
                     /locus_tag="I9760_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042323.1"
                     /translation="MKLRWATLAALTVIAALSFMAYYLSSHGDEVSSLTNVAEWQRMA
                     SPGELSKAHSFLSHNCTACHTPVKGVEAANCIVCHANNESLLKRQPTSFHANIGSCVE
                     CHREHQGRTARITQMDHNALVSLGLQQLRAFSNANDEEEQLIINQVTLFLAQNESNRP
                     TVLVNPHLSPQELTLRCASCHQNKDKHFKLFGDDCSACHETAHWSLSEFRHPSPSSMD
                     CAQCHQAPPSHYMEHFNMISKKVAHEHEARVDQCFLCHQTTLWTDIKGVGFYKHH"
     gene            complement(9522..10283)
                     /locus_tag="I9760_000532"
     CDS             complement(9522..10283)
                     /locus_tag="I9760_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042324.1"
                     /translation="MNAQGRMMVLGLVLLMLITWLGFAVHTSSRFAGSFWGGVFGVSG
                     SILMLISLLYLFIKRISFLKNWVTHYVSMNTLLSWHIYTGIIGSILVLIHTGHKFNSA
                     LGIALTAMTLIVTLSGFIGRYLMGFMTQEIDEKKTILLGLQKQYQIVAQELQESARSK
                     QNIGKLHLMTARFLSWLLEDTALDGQLIKSAEVRALCLADAISELEYAIRFHEHFKRL
                     FQKWLTCHIITALVLYVLLGLHVWSGIYFGLRWFQ"
     gene            complement(10283..12172)
                     /locus_tag="I9760_000533"
     CDS             complement(10283..12172)
                     /locus_tag="I9760_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HAT5042325.1"
                     /translation="MLFQHTKKFTLLQWVQTLSVFFLLAVASVSFAQPTADEHAAHHP
                     GEKEKEGEGMSGMMKNIGTPSKELYPSLMSLPTLSPEKRLAIQQKAHQRITSGMALMK
                     GGLNELLLASNNNDYAKMQEALEKLREGIAQYESALAALRALSEGKNPSDIALQWFKR
                     EMNLLPSSPQAESVLGGPFFHLTIIALFGLFFLVMIWMYFFKMRRAAALLDRLTKQNA
                     AVSSQTPPQFSKAPEPTPSLTSVEPIKSSQVSKVPSETAPTMPPTCPAHKCPVPQFPV
                     MRSLTEPEEKWEGQLRVCRIFQEAPGIKTYHLASTHEVALPFTYYPGQFITLTALING
                     KTIRRSYTMASTPTQLHYCAITVKREEQGLFSRYLHDEIKEGDLLEVMGPNGKFTFTG
                     EEAKSIVLICGGVGITPMMSIIRYLTDIGWHNDIYLLYCCRTTSEFLFREELEQLQER
                     YLNLHVYASMLRSEGTIWMGLQGLFTKNIISHLVPDIASHRIHVCGPPAMMEAILGSL
                     KELKVPADLILTEAFGPEKKPEIAQEDLVTIKAETRAMISFRKSEKMVPILPDCTLLE
                     IAEANGIAIDNACRTGQCGLCKVTLLSGEVTMACEDALSKEDKQQGLILACQAKATQN
                     IEVDA"
     gene            complement(12383..13252)
                     /locus_tag="I9760_000534"
     CDS             complement(12383..13252)
                     /locus_tag="I9760_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAT5042326.1"
                     /translation="MTKRTKLKEHVHTLEEIGHIMTAMKNLSLIETGKITQCLSMQDN
                     VIKTIREVSHDFLSFYPMPPMNQQENPPLVSILIGSERGFCGSFNDNVLHQLDAHKEQ
                     HSEWEPALVLVGHKLALKMANDSRVMATIDGPNAIEEIPAVISNVLHTLEKALLQKNK
                     TWHSWQWTILFNEEEHNQIQAKTWQPFKELDTSPAPHFSVPPVLNGSKDLFFADLVEH
                     YLLAMCYSIFYQSFFAENHQRLFHLNQTLDRLENKKNALNHRLNLLRQEEITEEIQNI
                     LQSAEAIIGHELT"
     gene            complement(13308..14780)
                     /locus_tag="I9760_000535"
     CDS             complement(13308..14780)
                     /locus_tag="I9760_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAT5042327.1"
                     /translation="MNWSNTPSFLEKQRQRLKRYQFQIKVSEQGRVVSVGDGIIWIKG
                     LPGAAIDEILISEDECCIAMVFHLTEELVGAVMLVQTKKLKAGTPIFPLKRVLSIPVG
                     DKLLGRVIDPLGHPLDGGEIPPHEEQGLLDRLSPPILHRDFVNRPLYTGNKMLDNLIP
                     IGKGQRELLIGDNGLGKSALALDIVMNQKDKKVYCVYVLIGQKRSTVSSTIQLLKEAN
                     ALDYTTVVVAQATALPGLLYLAPFAGCAIAEHWMKKGLDTLVVYDDLSAHANSYRELS
                     LLLRRPPGREAFPADIFYLHSRLLERSTCLSPALGGGSMTALPIIETKEGEMATYIPT
                     NLISITDGQIFFDESLFSSGFLPAIDITKSVSRVGGKAQHPQIKKESGRMKLDYLQFL
                     DLELFTRFGAKLDAKMQKQIQKGRVLREILKQERFSPLPIEFQLAWLIAYNEGFFDEL
                     NLEDIPKILKKIEEEIKQSNLSLGSSREQWKKAIKEWLMA"
     gene            complement(14767..15510)
                     /locus_tag="I9760_000536"
     CDS             complement(14767..15510)
                     /locus_tag="I9760_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042328.1"
                     /translation="MELSWTTFSLELINFLILIWILKRFLYAPIQKTILERKKRVQEQ
                     LETAQTLHRQATQLQTTYQNRLADWQQEKATLHNEWHEAMERWKSEERLQFEQQLRQE
                     KEQIFSHEMQKAAAIIENNAKEAFLLAGKFAEKLLMPFADAHLEEKIINKTIEDLNHF
                     PVEQWQWLNTVPEEETVSIQTAYPIQEHQKQDLLQVIEQLSPKKITVSFTENPKLLAG
                     LTLQMGPMCLQANLRDELKFFTETKNELV"
     gene            complement(15516..15791)
                     /locus_tag="I9760_000537"
     CDS             complement(15516..15791)
                     /locus_tag="I9760_000537"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAT5042329.1"
                     /translation="MNDISWFSLASTVIAAIAIAIGTIGPALAMGRAISHALDALARQ
                     PEAEKSITRTLFIGLAMIESLAIYCLVIVLIILFRNPLLSYFVTQQG"
     gene            complement(15788..16480)
                     /locus_tag="I9760_000538"
     CDS             complement(15788..16480)
                     /locus_tag="I9760_000538"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAT5042330.1"
                     /translation="MGEEGFLAHFAFSIGGLPITQSVLTTWFIMISLFIMAWSTTYKC
                     SLLQPSTYQLIWEGVLSTMYDAIKEVLPEHVELIFPFVATLWIFILVSNLIGVIPGFY
                     SPTADLSVTASLAIMTFLSVHWFGIRAEGWREYLKHYIKPTPFLLPFHLISEISRTLA
                     LAVRLFGNIMSLQLTALIVLMIAGFLVPIPILILHIIEAIIQAYIFGMLALIYIAGGI
                     QAHELKSQGESL"
     gene            complement(16483..16761)
                     /locus_tag="I9760_000539"
     CDS             complement(16483..16761)
                     /locus_tag="I9760_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HAT5042331.1"
                     /translation="MEFNDSKKELEQQVKRNVRKINKAKKGKSTLLAQTVYLGTLGFV
                     FVLPIIAGAYLGVWLDEKIKGYSISWTINLILLGVIIGAINVYLLIKD"
     gene            complement(16751..17161)
                     /locus_tag="I9760_000540"
     CDS             complement(16751..17161)
                     /locus_tag="I9760_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAT5042332.1"
                     /translation="MELFTIHLKSATQYEKIDKVISFVGEDASGQFGILAHHSRMMTC
                     LKFGLAWFRDENQETEYLALPGAVVYFIDNQLHIATRHYVRHKEYQAIQRVLEEELHW
                     EENNLLRTKESVHRLDEEMLKRLWELNRQNSYGI"
     gene            complement(17170..18594)
                     /locus_tag="I9760_000541"
     CDS             complement(17170..18594)
                     /locus_tag="I9760_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAT5042333.1"
                     /translation="MGHEYNWKMMDKNTVVAQRHGAPIGRIIEINGPVVKIRCDILPS
                     LHQSLKTYSDSDEYILEVCQHLDEHHVRAITLHRASGLQRGLVVYDQGTSLQIPVSKE
                     CLGRLLNIFGEPLDGAPPLETHEYRDVLVNSAPLEMTSTQETILETGIKVIDLLCPFV
                     RGCKTGLFGGAGVGKTVLLMELMHAIIQLHQGTSVFAGVGERIREGHELWHEMKSAGV
                     MDKTLMVFGQMDESPGVRFRTGLSALTYAEYLRDTLGHEVLFLVDNIYRFVQAGSEIS
                     GLLGRMPASVGYQPTLMTEIAELEERMTSTAKGAVTSVQAVYVPADDMSDPAVTGIIT
                     HLDSIIILSRSQAGKGIYPAVDPLASKSQFMDKILLGERHYSIAQAVREHLERYQELE
                     DMISMMGIEELSPKDRAIVLRARKLQRYLSQPFHVTKLQTGMEGKSVSLEHTLTDCES
                     FLRGDYDEFSEEECYMRGAMDKGR"
     gene            complement(18933..19373)
                     /locus_tag="I9760_000542"
     CDS             complement(18933..19373)
                     /locus_tag="I9760_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 domain-containing protein"
                     /protein_id="HAT5042334.1"
                     /translation="MLKRNVFILLTACGLVAAPLFAEDMSSGSQMNNMGMQSSNQQST
                     QLQEDMKQMQEALKQMQEMHDKLMNAKTTEEKKSLMQTQMPMMEEGMHTMQKGMKMMK
                     SMQDDAAKQPETMEQRTKMMQMMMQMMQMMMDRQMMGCPMMKNQ"
     gene            complement(20442..20705)
                     /locus_tag="I9760_000543"
     CDS             complement(20442..20705)
                     /locus_tag="I9760_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5042335.1"
                     /translation="MKKQTDLIVLGKQIRKIRKEKGFSQEGFANFIEMNRGYYGTVER
                     GEANITILNLLKILKGLEVTPNELFPPSTYVSLKRKITKNSAS"
     gene            21056..21598
                     /locus_tag="I9760_000544"
     CDS             21056..21598
                     /locus_tag="I9760_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate cyclase"
                     /protein_id="HAT5042336.1"
                     /translation="MKGIVFTSLNDMIIEQFGIETWDQLVSSLDLPSGGSYTAGGTYS
                     DTEFQQLIQAIAKRTNQHASVFLEAFGEYMFPILSSKYAIFLKKDMTLKEFLKSIDGT
                     IHVEVEKLYPDETLPTISYEEPTANQLVMVYRSHRRLCHFAMGLIQGAAQHFKKKITI
                     KQTHCMLKKDDHCRLEITFE"
     gene            21564..23084
                     /locus_tag="I9760_000545"
                     /pseudo
     CDS             21564..23084
                     /locus_tag="I9760_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
     gene            23263..25056
                     /locus_tag="I9760_000546"
     CDS             23263..25056
                     /locus_tag="I9760_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAT5042337.1"
                     /translation="MKGLEKQCCPKKRILTQSQELQEGVDFSCCAPADRGTSDNFFPF
                     FTRLVQANLAKWTAGISPAAIGSSYSTWLWQLAQSPGVLWELAFYPVFHAKDCINNIV
                     CVERAADGKDVRFKKDSWQPMPWRLFAEGFLQMEDWWRRATTDVPGLPNQVERTVSFW
                     ARQCLDALSPSNFVWSNPDLFHEAMRTGGLNLIQGGQIALEDWLEKLTGAPPTGSEHF
                     IPGKQVAITPGRVVFQNHLIELIQYEAQTKTVYKEPILILPAWIMKYYILDLSSHNSL
                     VKWLVSQGHTVFIISWRNPDKEDQDLGMDDYYRQGAMAAFDAVSTLFPETKINLMGYC
                     LGGTLAMITAAAMGRDKDERLNSLTLLAAQGDFTEAGELMLFVTESQVDFLKSMMREQ
                     GYLDTKQMAGSFQMLRAYDLIWSKMVQDYMHGMRRGMIDLTAWNADATRMPYKMHSEY
                     LEKLFLRNDFAEGRYTVEGKPVAAENIKLPVFAVSTEKDHVAPWQSVYKIHLMTEGDV
                     TFVLTGGGHNAGIISEPGHPGRSYRVHEQKQGEAYLNPESWLAMAERREGSWWREWNE
                     WLVQQNTKKRIASSVMNPSLPEAPGTYVLQK"
     gene            complement(25207..25950)
                     /gene="phbB"
                     /locus_tag="I9760_000547"
     CDS             complement(25207..25950)
                     /gene="phbB"
                     /locus_tag="I9760_000547"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HAT5042338.1"
                     /translation="MNQRVALVTGGTGGIGTAICQEMQKLDYQVIACYFKQGNHDLAK
                     EWQKKQAMQGFDIDIVYADISSFNDCEKLTDLVIEKYGKIDILVNNAGVTHDATLKKM
                     TQEQWQDVINANLNSVFNMTKTVLSNMLDNSYGRIITISSVNGRKGQFGQCNYAATKS
                     ALYGFTKSLAQEVAKKGITVNTVSPGYINTEMLASLRDDILEAIVAQIPVGRLGKPQE
                     IARVVAFLAEEQSGFITGANFDINGGQYM"
     gene            complement(26030..26239)
                     /locus_tag="I9760_000548"
     CDS             complement(26030..26239)
                     /locus_tag="I9760_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAT5042339.1"
                     /translation="MTNKIRGKVKWFNKDKGFGFIESSGKDYFVHFSSIQSDGFKTLP
                     DGATVLFKMGKGQKGPQAEEVEVIK"
     gene            complement(26335..26766)
                     /locus_tag="I9760_000549"
     CDS             complement(26335..26766)
                     /locus_tag="I9760_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042340.1"
                     /translation="MNDITSFKIKKIVLLKIIFAVIFSCTTLNACAGTQSYHIKYHKN
                     SKINNISLSNTEVLIEFHRNGNSLGRLLTKTNETQDITIDNTYGKNLVIHVLFIEKEG
                     KRIRCWGVSATKTNTIEIQCDKVVSLKKVEGNTEKATDSTH"
     gene            26951..27979
                     /locus_tag="I9760_000550"
     CDS             26951..27979
                     /locus_tag="I9760_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAT5042341.1"
                     /translation="MRLRLVILVLAAIIIGVGSYSGIVNVIKEPIENVIIGYKSPSAW
                     NNRGVDYVIGKRVAQNDSEAVKWFCKAAKQGEVMAQRNLGLMYAAGKGVPQDNGKAMQ
                     WFRKAALQNDAVSQLNLGVMYQKGMGTQQNDREAIKWIHKAAAQGFPEAERSLGILYS
                     IAENGQQNYVEAFKWLHKAAEKEDAIAQYNLAVMYVTGKGVRQNDTEAVKWFRKAGKH
                     GDLMAQRTLGLMYATGSNVQQDDFQAMKWFRLAAKQGDAVAQYNIGMGFLNGKGVIRN
                     HTKALKWFHLAASQGLPQAQYVLAALYHDGVSLPQNSMEAIKWLRKAAAQGHLQAQER
                     LQALVRQD"
     gene            complement(28361..29653)
                     /locus_tag="I9760_000551"
     CDS             complement(28361..29653)
                     /locus_tag="I9760_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042342.1"
                     /translation="MKHQTRIILAGTLGNLIESFDMAICGLLSVYIAKYLIGDVSKGL
                     FLVFLTFFAGYLARPIGAMIMGLLSDIYGRKIILAGSILTMGISTTLIGFIPPHSTIG
                     TFSAITLLVLRIIQSFSCGAEYLNSSAYLVENAEASKKGYSGSWASFGAMSGMLIASL
                     VVLIVTYFTSHYPEQDWMIWRVPFVLALLGSSIGLYIRLCIPESMEYIVYYADRPKPK
                     FKSLLSESVTYIKNNKIQSLYVFILSCLGVTTTFQIYIYGPMQARLYGNFQNHEIIIS
                     NIISLMVLLGVFPLIGNLSDKISREKIVVAASIGFLVLSQPFFNFLSHKIFYQLILSQ
                     ALIAIPAGAYYATVPVMLAEMFPIKLRCSVLSILYSIAASLSAGLAPLLSMILVKKTG
                     VASSPSILVFTLIATMFIIIIYRTIKPEKRCSLTTLKG"
     gene            complement(29658..30176)
                     /gene="def"
                     /locus_tag="I9760_000552"
     CDS             complement(29658..30176)
                     /gene="def"
                     /locus_tag="I9760_000552"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAT5042343.1"
                     /translation="MNTLLDKNNPILRQTADPISESEFGSSWLKELIKTMFGIMADKG
                     AVGVAAPQIGISKRVIVFGTDYTKRRKPEYPIPDTALINPSLKILSQEIQTGYEGCLN
                     CGELMGEVPRAMEIEYSGFDIDGNRITKKASGLEARILQHEIDHLDGFLFLDRVEDQD
                     SLTTLSAMQSKG"
     gene            complement(30160..30747)
                     /locus_tag="I9760_000553"
     CDS             complement(30160..30747)
                     /locus_tag="I9760_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgJ"
                     /protein_id="HAT5042344.1"
                     /translation="MDVTAVEVKLHPKAKEFLFEHFVTIRRVFSDVLGQVETDYASIA
                     LINQAGQIFFISSNPAIEQNLIEQSLWLFDGCYQPEFLSQDQPKLWSELPHIGCTESI
                     VKYKQIKPRLITGISIPTEYDSYKAIFSFGLKRINPYIQNKSSIHCEKLLAMGKFALR
                     RIQEYLIFPDKQPCMTTKPKLTLIINNQVPHEHTP"
     gene            complement(30761..31252)
                     /locus_tag="I9760_000554"
     CDS             complement(30761..31252)
                     /locus_tag="I9760_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5042345.1"
                     /translation="MSKGPYQSFANRLISALKDRGYTASRSPNGICIKTLAEFTGASE
                     QICRRYIRGDALPDYEKVKQLAFHLQVNPGWLLFGEDENATTKKNEVDEKLLHYILKQ
                     SHHLYPISQGSNDDYADFVLGLIKEVKAIDTSENNLLKIIDLAIGSISSYEEKRKKHS
                     HAV"
     gene            31461..31718
                     /locus_tag="I9760_000555"
     CDS             31461..31718
                     /locus_tag="I9760_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042346.1"
                     /translation="MSHNNKIGIYKGIIQYLLDATNYPLQRIANLSNSPIAYLQLIYH
                     HNRLLQNRSVELNLLKLFIVFIDMEIKREQKAEKLFPADMH"
     gene            31730..31933
                     /locus_tag="I9760_000556"
     CDS             31730..31933
                     /locus_tag="I9760_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAT5042347.1"
                     /translation="MQFSDNKMPKQILFITDLEELLGRNRLTLRRWWTSGKFPQPVKL
                     NGTTLAWHSETVNHWIQQNMRLS"
     gene            complement(31957..32898)
                     /locus_tag="I9760_000557"
     CDS             complement(31957..32898)
                     /locus_tag="I9760_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HAT5042348.1"
                     /translation="MRIAMNNPLIKYLKPALSFQDQLEKLQAKGLIINNWPSALQSLS
                     NTNYYRLSAYCLPFKRSDTTGNITEQFQDNVTFENVIDLYEFDRKLRLLVMDGLERIE
                     ISVRTSIAYHLAHSYGPFALSNPQNFHQQFEHNTWLAQINNEIERSREYFIEHYKNKY
                     LGYPNLPIWMAIEVLSFGALSVLFKGLKNEDKRIIAEGYKLHPKTLANWLYFLTYVRN
                     ICAHHSRLWNKDLAIKPKIDAINELWLPPITPRNDRSYIILLIIRKLLTTAGNGKDWA
                     ISSEKLIQPIIEKYDWAHESMGIPRSWLDHPLWKEVQ"
     gene            complement(32988..34169)
                     /locus_tag="I9760_000558"
     CDS             complement(32988..34169)
                     /locus_tag="I9760_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAT5042349.1"
                     /translation="MKFIDSYIKNLAPETTWFEKIESSGLGIRVMPSGNKSWFYRFSM
                     NGKRQKMTLGKYPAISLKQAREYLAKAQSLKEQGINPIENTKLEKLKEDNTFSKLIQS
                     WYENYAVKNRKQPRPIKYQIDSEIIPLLGDTVLDKLQTKDITIALDKIVQRGAPIHAN
                     RILSTIKQVLNYAVSRGYIQYNPATNIRSRDIGGIEKPRERVLSPEEIRIIWKFLESD
                     KSQMSESARLAIKVIILTGVRTGEIRLAQWHQFDFEQSLWTIPPEHSKGGITVKIHLS
                     ELTKKLLLQFKEQSVSPFVIPGITIDVPMSKDALPRAIKRIQNRVGIPEWTAHDLRRT
                     FATQLGESLNIDPVVIEKCLGHKMPRIMATYNKNEMLPQRKEALDSWANHIARLISSK
                     D"
BASE COUNT        10064 a         6664 c         7095 g        10644 t
ORIGIN      
        1 aactcaagac cagattatca agttaagtga cattttgcat ttaatgggcg aagtcaatcg
       61 ccttaaactt cttattgagt gcttaaaagg gccgaaatcg gtttcggatc tggcagagca
      121 attgcagctc tctgtccctt taaccagcca tcatttgagt ctgttgcgct cggcacggct
      181 cttgatggct aatcgcgaag gcaaacacat ttattacagt atttatgatg cgcatgtacg
      241 ctgtatttta gaggacatgc ttaaacattt tactgaagag acggagaatt aacatgcgtt
      301 tgctaaaaca agaggattgg ggtcgttatt cggtgagcca tgcttcgata ggaatgaatt
      361 gtctgcgatg tcgaacttct cttgctccgg gggcatattt ttacagtcaa tgtgggaaat
      421 catgatgaca gctccgctca ccttaaccac tttttttgtt gcaggacttg attgtcaggc
      481 tgaagaacaa ttaatcaggc ggcaattgca agctatccct gaagtggaaa agatggattt
      541 taattttatt gctgaagaag taacggttca tcatcgatta cccaccattg aacctataca
      601 acagcagata gaagccttag ggatgagcgt gcgcgccaaa caaagcaccc ctagcgacaa
      661 ggttcaggaa aaacttaaat ttgatgcctc ttggcagatt cttgcattat caggtctatt
      721 ggctctttgc tccgaaatag cagcttatct tttggcaaca gagcaatctc tatgggccat
      781 tattcctgcc ttactggcca ttattctcag tggacctcct acttttaaaa aaggttggct
      841 tgctttgcgc acaaaagcca tgaacattaa cagtctaatg ctcattgcca taacaggggc
      901 tatctttatc ggtgattggc ctgaggcggc tatggttact gttttgtttg cattggcgga
      961 acgaatagag cgttattcct tagacaaagc acgtctggct attcgaagct tgatgcagat
     1021 tgttcctgat gtggctcaag tgaaaacagc taatggtcaa tggcaaactt tactggtcga
     1081 ggaagtgcca ctgggtgctg tttttagagt taagcctggt gagcgtattc cattggatgg
     1141 tgaggtggtt tcaggtcaaa gtacagtcaa ccaagctcct attaccgggg aatccatgcc
     1201 agtgagtaag cagataggtg attctgtttt tgccggctct ttaaatgagc atggggcttt
     1261 tgaagcgaag gtgagcaaag cgtcaggcga tacgctgctc gctaaaattg gtaaagcgat
     1321 tgaacaggcg caagctgagc gggccccaac gcaacgattt gttgacgaat ttgcaagata
     1381 ctatacgcct attatggtat tgattgctgt tcttgtcgct ttaattcctc ctttcgtgtt
     1441 gggatattct ttttatgatt ggatctataa ggcgttgact ttattagtta ttgcctgtcc
     1501 ttgtgccctg gtcatttcaa caccagtaac agtggtgagt ggcttggctg cctctgccaa
     1561 acatgggctt ctcatcaaag gaggaagcta tttagagacg ggttatcgct tacagttaat
     1621 tgcactggat aaaactggta ccttaacaga gggcaaaccg gtggtaaccg attttattac
     1681 ttatgataaa agtcaaacca atgaacactt gttgttgctt gctgcaagtc ttgacagcca
     1741 ctccgagcac cccgtagctc atgccctagt ggcatattgg cagcaagagc atcctgataa
     1801 atcattgctt tcagtggaac ggttttcagc gcttcctggg cgaggcgtga aaggtgccat
     1861 ccagggggaa ttgtattatg taggcaatca tcaattagca gaagacaaca aagtctgttg
     1921 tttagaggtt gagcaagaat tgaaacgact ggaagaagaa ggaaaaacta ctgttatatt
     1981 aagcaatagc actacggcct tggctatttt tgcagtggca gatacgcttc gagccaacag
     2041 tcgagaggcg attgaatcat tgcatcgaca aaggataaaa acggcgatgt taacaggtga
     2101 taatgctcta accgctcaag ccattgccaa acaagtcggc attgatgaaa tccaagctaa
     2161 tatattacca acagaaaaaa tgcaggctat caataaactc ttagaacaat atcaggctgt
     2221 gggcatggtg ggagatggca ttaatgatgc acctgcttta gcgaaagcca ctattagctt
     2281 tgcgatgggt aagggaacag ataccgcttt agagacagcg gatgtggtat taatgaatga
     2341 taatttagcc atgcttcctc tgtacattga cttaagccgg aagacagtgc gaattttatg
     2401 gcaaaacatt agtctatcct tggttattaa aacggtgttt tttattttag cacttggagg
     2461 aatggctacc ctttggatgg cggtttttgc tgacatgggg gctagtttga ttgtggttat
     2521 gaatgggcta cgattgttga attcaaataa aacgaagaat cattaataca atcgctttga
     2581 ttgctttttc aatatgttat gatgagataa accactgttt ttgtatttgt tgaaagggag
     2641 aggtagatga ataatactgg atttaagaca atttcctttt tgattggagt attgttttag
     2701 gatgtagctt aatgtcctgc actaaaacca ccgcgacaga aacgcatcct gaaagcggtt
     2761 acgagggagg ttttggtggt gatggagggc ataggcacca agagcattaa aacctctata
     2821 gtcagataag gatagattat tatgggagtt aatactttga ctatagtatt gctattggca
     2881 ttgatggtat tatgccttgg ccttcttgcc tttctttgga aaaaaaagaa aaatttgcgc
     2941 tttgttataa ccatattaat cttccttatc ctagctgcca tttttttaca tggtgtttcc
     3001 ttccaattgc attaggtctt ttttattcat tttaggaaat tatacgatga taaagattag
     3061 tataggggtt ctaaacgcta accgtcgaaa tttccgtgca ataattaatc atcccaaaat
     3121 tctttaaatt ttacggaagc aatttctgtg taccttacgg tatgttgaat atttttatga
     3181 cccaaataat gttgaatact gcgagtatcc cgaccgtcat tagctagttt gaagcctgtt
     3241 gagtggcgaa gcatatgagg gtatgtcgcc aagccaagtt ctgctttttc tcctgctctt
     3301 gcgataattt ttctaaaagt atctgcagta ataggtgctt tacgctccgt cataaaaaca
     3361 taatcggttt ctgggtaatc gcgttttaat tgacgcagag cccgaatctc tgggccaaaa
     3421 agagggtgac tggctgggat tccatttttt ttgcgaatca catgaagtag accttgtttt
     3481 aaatctattt gagaccaacg taatgagatc aattcagata cgcgtagttc atgtcgatag
     3541 gcaattaaaa tcattgtaga gtcacgtaag ccatgccgcc ccaattgctt tgcagacgca
     3601 ataagcttat ccacctcagc tggagtaagg tattctcgtg ctctgcgctc actatttttt
     3661 acttttttag gcggtttcat gattcacttt tatacacttt ccctaaaaga gttattggtg
     3721 tcacttccag gcatccaaac aacatgttta tttataagac aattcacgat ttaattttag
     3781 caaactttcc gataaaggta ttctttaggg agagtttttt agtaacatat tgtctctttt
     3841 ttaattaaga ttttttatca tgcgtgcaag caatgaaaga ttaaccattt tatctgaggc
     3901 agagcaagct gctttgtatg agatccctga ttttgatgat gttcagcgct cgaactatct
     3961 caatctcacc cctgaagaac aaatcttgat gcgtagccgt gataatttat caactaaagt
     4021 ccattgcgct attcaaatag gttacttcaa ggcgaaacat cggttctttc catttaagtg
     4081 ggacgaggta caggaagata ttgatttcat tatgcaggaa tattttcctg aacaacaatt
     4141 tcttccaacc cctatcagca aacaccaata ttatgcacaa tgcactgcga ttacgacgca
     4201 ttttggttac cagtcttggt caaaagagtt tgaaccatta ttgactgccc aggcacaaca
     4261 gattcttcgc cgtgatgtta gtccacaatt tatcgtcatc gaattactag cctttatgca
     4321 gaaaaagaaa atcttgcgtc ctggatatac cactttgcaa acgattgtga gcaaagtctt
     4381 aaatgctgaa cgcaatcggc tagaggtaat tcttagagaa tccctgacga tagatgataa
     4441 atccgctttg caaaaattgt tgctagaaaa agaagcccaa acgctttctg gattagcaga
     4501 tctcaagcag gataccaagg attttaaagc acgcatgatc agtgctgagc gcgaaaaatc
     4561 gatttcgata aaacccctgt atcagttagc taaatcgctc ttgcctacac ttaatctttc
     4621 tcagcaaaat attcgctact acgcaagtct tgttgattat tatgatgtct atgaattacg
     4681 gaagaaatta aaatctgagc agacctatct ttatcttctg tgttatctct ctttacgata
     4741 ccttcagttg aatgataatt taatagacgc ttttttcctg agcctcaagc aatttgaagc
     4801 agagcttaaa gaaaaaacaa aagatgccta tacggactac gcggtaagca aacaaattga
     4861 ttcttttgtt atgcgtcgat ttgcacggtt atttatcaac caagagctac atgatcaact
     4921 cagctttggc gatgtacgca aaaaagcatt tgaaaccatt atccctgaag aagagcttcg
     4981 ccataaaata cccgaagaaa atgaggagga gcttaagcct atcgattttc aatggaaact
     5041 ggttgatact ttatttcatc gatgtcagtt gcagttacga ccattaatga tggccattga
     5101 tttctcaagt actgcagatg aaagtccttg gcttaaggca attacctggc ttaaggagct
     5161 atttggtgcc aacaaaacga taaaccaata tccagtcacc gcaaaccccg aaaaaaccag
     5221 gcaaaaacgc ttggaaaaat acttgtttga tgcgaatgcc aagggtgaga aaaaattcca
     5281 tgccgatcgc tatgaatttt ggatttatcg tcaactgaag aaacggctta aagcaggaga
     5341 attctatttg gcagacagtg tacagaatcg ctcgctttaa caagagctta attcagccaa
     5401 tgacaaaggg gcgctagtgg aacccttaga tattccagca ttgagaaagc ctattaagga
     5461 cttactagac gaacgttgtg ctgaattgcg atcagaatgg ttgaaattta atactgattt
     5521 tactcaaggt aagctcaagc atctgtacca tgatgaacaa acccaaacgc tccatctaaa
     5581 aaaatcgagt gatgacaaac gcgatgaaga tgctcaacac cgtttctatg aacaattgcc
     5641 cttgtgtgat attacggatg tacttcgatt tgttaatgaa cggagtcgtt attcatctgc
     5701 ttttaccctc atacaacctc gctatgttaa attgcttgcc gatgaaaaca ctctgaatgc
     5761 agtgattatc gctcaagcgt tgaataacgg caatcttaat atggcagaaa tttctgatat
     5821 tccttatgcc agtttgttgg acgtttacca atcccgtgtg cgattgcaaa cattaaaaaa
     5881 agccaacgat atgattgggg atgatattgc acaaatgcct atttttcctg attactcgtt
     5941 ggatatggct attctttatg gtggtgttga tggccagaaa tttgaagtgg caagacctac
     6001 actgaaagca aggcgttcaa aaaaatattt taaaaaaggg aaaggcgttg tggcttatac
     6061 gctgctagtc aaccatatcc cgctgcaaac tgaactgatt ggtgctcatg aacatgaaag
     6121 ttatttcact tttgacattt ggtataataa caccagcagc gtcatacctg atgccattac
     6181 aggtgatatg catcttatta ataaagctaa ctttgctgtg atggactggt ttggtggtaa
     6241 actgtgcctt agatttacca atttgcaagc ccagctcaag catctttact gtgatgatga
     6301 tctatcacaa tatgcagatt ggttaatcaa gcccgtagac aaaattgatc gccaacttat
     6361 tgaggatgaa tggcctaatc tggaaccgat tatcaaagcg ttaggctgta aagaaatcac
     6421 tcaaagcatt ctgattaaaa aattatgcac gtacagcgcc tcaaacaaaa ccagaaaggc
     6481 attatttgaa tacgataaat tgatccgtag catctatacc ctgaaatatt tgcaaaacag
     6541 aaaattgcaa cgagacatac atcgttctca aaatcgggta gaatcctatc atcaactacg
     6601 tatggctatt gcaaccgcct atggtaaaaa gcaattatca ggcaggggag atcgtgaaat
     6661 tgagatcagc aaccaatgtg ccaggcatta ttgctaatgc cattattcat tataattcgg
     6721 caattctatc caagctcaaa ctcaaatatg aagctgaggg agatcataag gcattagcac
     6781 tgttgaagaa aatttcgcct gtagcttggc aacatatcca ttttcaggga cactttattt
     6841 tttctggtga taaaatcatt gatctcgatg caataataaa caaagttttt ttgcatggag
     6901 taaaaaagaa aaaagtggtt gaatttagtc cggaaatttc gacggttagc gtttagaacc
     6961 ccttcaacgt ctttaaagtc cacatattgc cgctccttgt aaaagagctg attaagctac
     7021 tctaaatcca atacttatct atttaagtat agacaagata gcactgaagt tcataaattg
     7081 atcaatttat gaaatgatac ctgactggcg caatccttta gaaggatgaa ctatactaat
     7141 acaaattact gagtcaaagg agtgcacatc atgtcgcctg catttgggca ccacccgaaa
     7201 tcaccaaaac cacaaaaaga gaagggcttc tggagttctc cttccggtct aatggccatc
     7261 atcattattg ggattattgg ctattacttg attgtagagc atggtgctca catagcaagc
     7321 tttttaggcg cttcaccatt ggtgctgtta gtcttactct gtccattgat gcatcttttt
     7381 atgcatagag gacatggggg gcatggagag aatcatcacc ataaatcgga tgacgaggac
     7441 aacgattctt ctgagaataa ggactaatcg aaaatggaac atacttctta tgcctatggt
     7501 ttgtggttat tagtcattgt caattcggcg attttcattt tgtttgcata cagttttacc
     7561 accacgttta aaacaaggcg agattggcgc gcctttggtg cattttctgc gtttgttatc
     7621 gcttatttta ccgaaatgta tggttttcct ttaaccattt atttgctgtc aggatggctt
     7681 tctaaatatt atcctggaat ggatttatat ggtcatgaca gtggccattt actccatacc
     7741 ttgcttggct tgaaaggtga tccccatttt gatgtctttc atatcttaag ttttgtcttc
     7801 attatcggcg gtctttggat gattgcatca gcctggggta ctctttatcg tgctcaaaag
     7861 gcgcatcaac ttgcaaccag tgggctgtac gccaaaattc gtcatccgca atacgatggg
     7921 tttattctgg tgatgattgg ttttttattg caatggccaa ccattctgac cttaatcatg
     7981 tttcctatat tagtttatat gtatgttcgt ctcgcgcgta gggaagaaaa agaggttttg
     8041 gcggagtttg gtgaggaata taaacactat gctgcagtta ctcctgggtt tatacctcgt
     8101 attagtaaac aacaagacag caaaggaggt gcaccatgat gtttatgttt cacagtgccg
     8161 agatgctcgg ccttatcgca atagcgcttg gaatcatgtt ggtcgtctgg gcattacgta
     8221 atgaaggaaa aagtattgga ttagccaaaa tatttggatg gttgattgtc attatcggga
     8281 ttctagggga gctatgcagt acttattatg cgattaaata ctggtatgca ggctatttcc
     8341 agatgccaat gatgtcaggt gaagcattct tatttcatgt ggctcagatg ttgtctctta
     8401 ttgcaatagc attgggtatt attttgatag catgggcgtc acataacgaa ggacgtggta
     8461 tttcgctagc aaaagtcttt ggttggttca tcgttctctt tgcgattttg ggcatgactt
     8521 gtagtgctta ttatgccact gtgtattggt ataaaggata tattcaaaca cctgctggta
     8581 tgcctatgtt gttgcaacat caaataccga tgcagaaaca gtcacccaat caatagtcct
     8641 ggtaagtgcc ataacaaatt gttacggcac ctataaaatt ttccgttgta gagaaagagt
     8701 ccctcaatga tgcttataaa atccaactcc tttaatatca gtccacaacg tggtttggtg
     8761 gcaaaggaaa cattgatcga cacgggcttc atgctcatga gccacttttt ttgaaatcat
     8821 attaaaatgt tccatataat gactgggtgg cgcttgatgg cattgagcac aatccattga
     8881 actcggagat gggtgcctaa attcagagag gctccaatga gccgtttcat gacaagcaga
     8941 acaatcgtcg ccaaataact taaagtgctt gtctttgttt tgatggcaag aggcacagcg
     9001 taaagtcagt tcttgaggtg ataaatgggg gttgaccaat acggttggcc tattggattc
     9061 attctgcgct aaaaagagcg tcacctgatt aataatcaac tgttcttctt catcatttgc
     9121 atttgaaaag gcacgaagtt gctgaagtcc taacgaaacc aaagcattgt gatccatctg
     9181 cgtaatacgt gccgttctgc cctggtgctc tctatgacat tcaacacaac tgcctatgtt
     9241 ggcatgaaag gaggttggct ggcgttttaa cagagattcg ttgttggcat ggcagacgat
     9301 acagttcgct gcctcaaccc ctttcactgg agtgtgacac gcagtgcagt tgtgacttaa
     9361 aaacgagtgg gctttggaaa gttctccggg gcttgccatt ctttgccact ccgccacgtt
     9421 cgtcaaactg ctcacctcat ctccatgaga agacagataa taggccataa aagacagtgc
     9481 cgcgattacg gtaagggcag cgagggtagc ccatctcagc ttcattgaaa ccaccttaat
     9541 ccaaaataaa ttccagacca aacgtgcaaa cccagcagaa catacaatac cagtgctgta
     9601 ataatgtgac aggttaacca tttttgaaac aggcgcttga agtgctcgtg aaaacgtatg
     9661 gcgtattcca gctcagagat ggcatctgct aaacacaaag cgcgtacttc tgccgacttg
     9721 attaactgac catccagtgc ggtgtcttca agcagccagg aaagaaagcg agctgtcata
     9781 agatgtaatt tcccgatgtt ttgcttggag cgtgcgcttt cttgtaattc ttgagctacg
     9841 atttggtatt gcttttgtaa tcccaaaagg atagtctttt tttcatcgat ttcctgagtc
     9901 ataaagccca tgagataacg gccaataaaa ccactgagcg tgacaatgag cgtcatagca
     9961 gtcaaagcaa tacctaaagc gctattaaat ttatgccctg tatgaatcaa gactaaaatc
    10021 gagccgataa tacctgtgta aatatgccac gacaacaggg tattcatgga cacatagtgt
    10081 gttacccaat ttttcaaaaa tgaaatacgt ttaataaaga ggtagagtag cgaaatgagc
    10141 atcaatatcg agccactcac gccaaaaacg ccaccccaaa agctcccggc aaaacgagaa
    10201 gatgtatgga ctgcaaagcc tagccatgtg ataagcatta gaagtactaa ccccaaaacc
    10261 atcattcgtc cttgcgcatt cattaagcat ccacctcaat gttttgagtc gctttggcct
    10321 gacaggctaa gataagcccc tgttgtttgt cttcctttga aagcgcatct tcacaagcca
    10381 tggtcacttc ccctgacagc aaggtcactt tacataaccc gcattgcccg gttcggcacg
    10441 cattatcaat agcaatccca ttggcttcag cgatttcaag aagggtacag tccggcagaa
    10501 ttggaaccat tttttcagat ttccgaaaag aaatcatcgc acgggtttcg gctttgatgg
    10561 tgactaagtc ttcttgagct atttctggtt tcttttctgg tccaaatgct tctgttagaa
    10621 tcaaatcagc gggcacttta agctctttta agctaccaag gatggcttcc atcatcgcag
    10681 gaggaccgca aacgtggata cgatgtgagg caatatcagg gacaagatgg ctgataatat
    10741 tcttggtaaa taggccttgt aaccccatcc aaatggttcc ctctgaacgc agcatcgacg
    10801 catacacatg gagattgagg tatctttctt gaagttgctc taattcttcg cgaaagagaa
    10861 actcactggt cgtgcggcaa cagtacagca aataaatgtc attgtgccat ccaatatcgg
    10921 tgaggtagcg gatgatactc atcatcgggg taataccaac cccgccacaa atcaatacga
    10981 tgctctttgc ctcttcacca gtaaaagtaa atttaccatt aggccccatc acctccagta
    11041 aatccccttc cttaatttcg tcatgcaaat aacgggaaaa caagccttgt tcttcccgct
    11101 tgaccgtaat ggcgcaataa tgcaattggg ttggggtgga ggccatggtg taactgcgtc
    11161 ttatcgtttt accattaatt aaggcggtga gcgtaatgaa ctggccagga taataggtaa
    11221 atggcaaagc cacctcatgg gttgatgcca aatgatatgt tttgattcct ggtgcttctt
    11281 gaaagatacg gcaaacacga agttgtcctt cccatttctc ttccggttcg gtgagtgatc
    11341 tcatgaccgg aaattgcggg actggacatt tatgagcggg gcaggtagga ggcatagttg
    11401 gggcagtttc tgatggcact ttagaaactt gagaggattt aatgggctct actgatgtta
    11461 aggaaggggt tggttcaggt gcttttgaga attggggtgg tgtctgcgaa gatacagccg
    11521 cattttgttt ggttaagcga tcgagcagag cagcagctct tctcattttg aaaaaataca
    11581 tccaaatcat gaccaaaaaa aacagaccaa atagagcgat aatcgttaaa tgaaaaaaag
    11641 gcccacctaa gacactctcg gcttgcggac tacttggaag aagattcatt tctcgtttaa
    11701 accactgcaa ggcgatgtcg cttggatttt ttccttcaga cagtgcgcga agtgcggcca
    11761 gtgcactctc atattgcgca atcccttccc gtaatttctc taaagcttct tgcattttag
    11821 cgtaatcgtt attgttggat gccaacaaaa gctcgttaag tccacctttc atgagcgcca
    11881 tgccggatgt gatgcgttga tgtgcctttt gttgaattgc aaggcgtttt tcgggggata
    11941 atgtgggtaa actcattaaa gaggggtaaa gttccttaga gggagtccct atatttttca
    12001 tcataccact cattccctcg ccttctttct ccttttctcc tggatgatgt gcggcatgct
    12061 catccgctgt gggttgagca aaggagactg aagccaccgc caacaggaaa aaaacagata
    12121 atgtttgaac ccattgaagt aaggtaaact ttttagtgtg ttgaaacagc atcctttccc
    12181 tccaaagccc acttaacatg acgattcaaa aatggttaaa gtggagcagt ccgtacgagt
    12241 gtttccccgc ctcatatgag tttattcact attctgtaac cgatttccaa tcactcaaag
    12301 ggatgactac gaattcatag aaaattcatt tcatggatta gaaacccagt tcaatcagta
    12361 tattatagtt taccttgtct ttctatgtga gttcatgacc aataatcgct tcggcgcttt
    12421 gtaaaatatt ctgaatttct tcggtaattt cttcttggcg cagtaaattg aggcgatggt
    12481 ttaatgcatt ttttttattt tccaagcgat ctaatgtctg gtttaagtga aacagtcgtt
    12541 gatgattctc cgcaaaaaac gattgataaa aaatcgaata acacatggca agtaaataat
    12601 gctccaccaa gtcagcgaaa aacaggtctt tggaaccatt taaaacgggt ggcacagaaa
    12661 aatgaggcgc aggacttgta tccaactctt taaagggctg ccaggttttt gcttgaattt
    12721 gattgtgctc ttcctcattg aagaggatag tccattgcca agaatgccat gttttatttt
    12781 tttgcaataa ggccttttct aacgtgtgta acacgtttga aatcacggct gggatttctt
    12841 caatggcatt gggtccatcg attgttgcca tgacacgaga atcattagcc atttttaatg
    12901 ccagtttatg ccctacaaga acgagtgctg gttcccattc agaatgttgc tctttgtgcg
    12961 cgtctaactg atgaagcaca ttatcattaa aagaaccaca aaagccgcgt tctgagccaa
    13021 taagaataga aactaaggga ggattttctt gttgattcat ggggggcatg ggatagaaac
    13081 ttaaaaaatc atggctcacc tcacgaatgg ttttaatgac gttatcctgc atggaaagac
    13141 attgagtgat tttgcctgtt tcaattaaag aaagattttt catggccgtc atgatgtggc
    13201 caatttcttc taaagtatga acatgttctt ttaatttcgt tcgctttgtc ataaaacctt
    13261 ccaaggtcaa tgagactcag tactttggaa tggataatga gtccgttcta tgccattagc
    13321 cattctttta tcgctttttt ccattgttct cgtgagcttc ccaaggataa attgctttgt
    13381 tttatttctt cctctatttt tttgagaatt ttggggatgt cttctaaatt caattcgtca
    13441 aagaaaccct cattgtaagc aataagccat gcgagttgaa attcaatggg tagtggtgaa
    13501 aaacgttctt gttttagaat ttctcttaaa actcgccctt tttgaatctg cttttgcatt
    13561 ttggcatcca gtttcgcccc aaagcgggtg aacagttcca aatcaagaaa ttgcaggtaa
    13621 tccagtttca ttcgaccgga ttctttttta atttgcggat gctgcgcttt accaccgact
    13681 cgtgagaccg atttggtgat atcaatggcg ggaagaaatc cagaagagaa taaagattca
    13741 tcaaaaaaga tttgcccatc ggtgatggag atgagattcg ttggaatata agtggccatt
    13801 tcaccttctt tggtttcgat aataggcaac gccgtcatac taccgccgcc cagagcaggg
    13861 gaaagacagg tggagcgttc taataaacgg gaatgcagat aaaaaatgtc cgcaggaaat
    13921 gcctcacgtc cgggaggtct acgcagtaaa agggacagtt cacgataact attggcatga
    13981 gcactcaaat catcatagac caccagggta tctaagccct ttttcatcca gtgttcggca
    14041 atggcgcacc ctgcaaaagg agccagataa agtaatcctg gtaaggccgt cgcttgggct
    14101 actacaacag tcgtatagtc caaggcgttg gcttctttta ataattgaat ggtagagctt
    14161 accgtggaac gtttttggcc aatcaacaca tagacacaat acactttttt atctttttga
    14221 ttcatcacaa tgtcaagggc caaagcactt ttaccaagcc cattatcccc aatcagtaac
    14281 tccctttgtc cttttccaat gggaattaga ttatcaagca tcttatttcc tgtgtacaat
    14341 ggtcgattca caaagtcacg gtgaagaata ggtggggaca gcctatccag taatccttgt
    14401 tcttcatggg gcggaatctc accgccgtct aatggatggc ctagaggatc aatcactcgc
    14461 ccaagcagtt tatcacctac aggaatactc aatacgcgtt tcagaggaaa aataggggtg
    14521 cctgccttta attttttggt ttgtaccagc atcacagcgc caaccagctc ttcggtgagg
    14581 tggaacacca tcgcgataca gcactcatcc tctgaaataa gaatttcatc gatggcagcc
    14641 cctggtaagc ccttaatcca gataatccca tctccaacag aaaccacccg cccttgttcg
    14701 gataccttga tttgaaattg atagcgctta aggcgttgtc gttgcttctc aagaaaagaa
    14761 ggggtgttag accaattcat tttttgtctc tgtaaagaat tttaattcat cacgaagatt
    14821 ggcttgcaag cacatggggc ccatttgcag ggttaatccc gcgagaagct ttggattttc
    14881 cgtaaaagag actgtaattt ttttaggtga caattgttca ataacttgca agagatcctg
    14941 tttctgatgt tcttggatgg ggtaggccgt ttgtattgaa acggtttctt cttcaggcac
    15001 agtattgagc cattgccatt gttctaccgg aaaatgattc agatcctcaa tggttttgtt
    15061 aatgattttc tcttcaagat gagcatccgc aaaaggcatt aacaattttt cagcaaattt
    15121 cccagccaaa agaaatgctt ccttggcatt gttttcaatg atggctgcgg ctttttgcat
    15181 ttcatgggaa aaaatctgct ctttttcttg acgcaattgt tgttcaaact ggagtctttc
    15241 ttctgatttc catcgttcca tcgcctcatg ccactcgttg tgtagggtcg ctttctcttg
    15301 ttgccaatcg gcaagacgat tttgataggt tgtttgcaat tgcgttgctt gcctgtgcag
    15361 tgtttgtgca gtctctaatt gttcttgcac tctttttttt cgttctaata tggttttttg
    15421 tatgggcgca taaagaaagc gtttcaaaat ccaaatgaga ataagaaaat taatgagttc
    15481 taatgaaaaa gtggtccaag agagttccat tggttttatc cctgttgcgt tacaaaataa
    15541 gacaataatg gatttctaaa gaggataatc aaaacaatca ccaaacaata aatggctaac
    15601 gattcgatca tggccaaacc aataaataat gttctggtga tggatttttc ggcttcaggt
    15661 tgccttgcca aagcatctaa ggcgtgactg atcgcgcgcc ccatggccag agctggccct
    15721 atagttccta tggcaatggc tattgctgca ataacggttg acgctaaact aaaccaactt
    15781 atgtcattca taacgattct ccttggcttt taagctcatg ggcttgaatg cccccagcaa
    15841 tgtaaattaa ggccagcatg ccaaaaatat acgcttggat gatggcttca ataatatgta
    15901 aaatgagtat tggaatagga actaaaaatc cggcaatcat gaggacaatc agggcggtta
    15961 attgcaagct catgatatta ccaaacaagc ggacagccaa tgctaaggtt cgagagattt
    16021 cgctgattaa atgaaaaggc aacaaaaaag gagttggctt aatataatgt ttcaaatact
    16081 ctcgccaccc ctcggcacga attccaaacc agtgtaccga taaaaaagtc attatcgcaa
    16141 gggatgccgt caccgataaa tccgccgttg gtgaataaaa tcccggaata acaccaatta
    16201 aattggaaac aaggataaaa atccacaagg tggctacaaa aggaaatatc agttcaacgt
    16261 gctcgggcaa tacttctttt attgcatcgt acatggtgct caaaacaccc tcccaaatga
    16321 gttggtacgt gcttggctgt aggagagagc atttgtaagt ggtactccat gccatgatga
    16381 ataaggagat cataatgaac cacgtggtta agacgctttg tgtgatgggt aatccaccga
    16441 ttgaaaaggc aaaatgcgct aagaatcctt cttctcccac ggttagtcct taattaaaag
    16501 atacacgtta atggcaccaa taataacccc aaggaggatt aaattgatgg tccaacttat
    16561 ggaataccct tttattttct catcgagcca aaccccgaga taggctcctg caatgatggg
    16621 taacacaaaa acaaagccta aagtacccag gtaaactgtt tgggctaata acgttgattt
    16681 tcccttttta gctttattga ttttgcggac gtttcgtttc acttgttgtt ccagttcctt
    16741 tttcgaatcg ttaaattcca tagctgttct gccggtttaa ttcccataaa cgctttaaca
    16801 tttcttcatc caaacgatgc acgctttctt tagtgcgcaa tagattgttt tcttcccaat
    16861 gtaattcttc ctccaggacc ctttgtatgg cttggtattc tttatggcgc acataatgtc
    16921 gtgtcgcaat atgcaattga ttgtcaatga aataaaccac ggcacctggc aatgctaagt
    16981 attccgtctc ttgattttca tcacgaaacc aggccaagcc gaattttaag cacgtcatca
    17041 ttcggctatg atgagctaaa attccaaatt gcccagaagc atcctcaccc acaaaactga
    17101 tgaccttatc gattttttca tattgtgtgg cactcttaag atggatagta aaaagctcca
    17161 tgtccgtcgt tatctccctt tatccatggc acctctcatg taacactctt cttctgaaaa
    17221 ttcatcgtaa tcgcctctta gaaaggattc gcaatcggtt aatgtgtgtt ctaatgaaac
    17281 cgatttccct tccattcctg tttgaagctt ggtgacatga aagggttggc taagatagcg
    17341 ttgcagttta cgtgctctta acacaatagc acggtcttta ggagaaagct cttcaatccc
    17401 catcatagaa atcatgtctt ctaattcttg ataacgttcc aggtgttctc gtacggcttg
    17461 tgcaatggaa taatgtcgct cgcccaatag gattttatcc ataaattgac tcttggaagc
    17521 taagggatcc acggcaggat aaatgccttt acctgcttgt gaacgagaga gtatgatgat
    17581 ggaatccaaa tgagtaataa ttccggtcac agccggatcg ctcatatcat cggctggaac
    17641 ataaactgct tgtaccgaag tcactgctcc tttggcagtc gacgtcatgc gctcttcaag
    17701 ttccgctatc tcagtcatta aggtgggttg ataaccgaca cttgccggca ttcggccgag
    17761 taggcctgaa atttcactgc cggcttgcac aaaacgataa atattatcca caagaaaaag
    17821 aacctcatgg cccaacgtat cacgcaaata ttccgcataa gttaatgccg ataaccctgt
    17881 gcgaaagcgc actcctggcg attcatccat ttgaccaaat accatgaggg ttttatccat
    17941 aacgcccgcg gatttcatct catgccacaa ttcatgtcct tcccgaatgc gttccccaac
    18001 accagcaaag acggaggttc cttgatgcaa ttgaataatg gcatgcatca attccatcag
    18061 tagtacggtt ttcccaactc ctgcaccacc aaacaatccc gttttacacc ctctgacgaa
    18121 agggcaaagt aaatcaatga ctttaattcc agtctcaaga atggtttctt gagtgctcgt
    18181 catttcaagt ggcgcggagt tgaccagaac atcacgatat tcatgggttt ctaatggcgg
    18241 ggcgccgtct aaaggctcac caaaaatatt taaaaggcgg cccaagcact cttttgaaac
    18301 gggaatttgt aaggaagtgc cttgatcata gactactaaa cctctttgca atcctgaagc
    18361 gcggtgaagc gtgatggctc tcacatgatg ctcatcaaga tgctgacaca cttccaaaat
    18421 gtattcatca ctgtccgagt aagttttaag cgattgatgc aaagaaggta aaatatcaca
    18481 acgtattttg acaacagggc cattgatttc gattatcctg ccgatggggg ctccatgcct
    18541 ttgagcaaca accgtgtttt tatccatcat tttccaattg tattcatgac ccatgaatta
    18601 ggtgtcgtcc tattctctct atttttacat cactttatgg aatttaatat aacagagttt
    18661 gaaaacagat agcaaaatgc taattaataa cagctcgcct gaaagtatct aaaaaacaaa
    18721 gcactgtgtg aaggtctcat taatcagctt ctacataggt cactgtgcca catgatacac
    18781 gaagctctcc tttaaataga gttcgacgaa ataggcaggt tctgaatggg gtaatcttaa
    18841 tgagctaccg gcctataata gggctcatag aaatctcata gagccctggt gattggattt
    18901 aattgcccta gtataggcct cttatagaac atttattggt ttttcatcat ggggcatccc
    18961 atcatttgtc tatccatcat catctgcatc atttgcatca tcatttgcat cattttcgta
    19021 cgctgttcca tcgtttcggg ttgttttgct gcatcatcct gcatactttt catcatcttc
    19081 atgccttttt gcatcgtatg catgccttct tccatcatag gcatttgtgt ttgcatcaat
    19141 gatttctttt cttctgttgt tttggcattc atgagcttat catgcatttc ctgcatttgt
    19201 tttagtgctt cctgcatttg tttcatgtct tcttggagtt gggttgactg ctgattagag
    19261 ctttgcattc ccatattatt catttgagaa ccagaagaca tgtcttctgc aaagagtggc
    19321 gcggcaacca aaccgcaagc agtcaataaa atgaatacat tccgttttaa cataatgaaa
    19381 tctccttatt attttttcaa tgaattgttt ggactgagca tcctactata gcactacatg
    19441 aacaaggtat gaaaagcaaa aaacatactt gctcatgtta atgccaacct attcctcccg
    19501 tattgtgtcc ttcataagta tgagaaacac tatgtgaatt attggttgtt gttaatgggg
    19561 cgcaagagat caacaaaagt atcgtgctca ttatcccaat aactgcttta ataagtctct
    19621 gctttggcat atccatgctc cttgcaatga aatcatgtga tggctgaatc actagtaaaa
    19681 taacccatct cattttataa atctttttta taatatagca ctgtttagaa atacgcagtg
    19741 ttagtcacca actacgtatt gaaaaaatca gttgtctttc ggcaataggg aatcgatacc
    19801 tccccaagct taatataagt tcaaattatt atttgaattc actgccaagg atgtgaatag
    19861 atgttggagt taaggaagac gataagtagg gtatttaatt attgcgaaga ttctaccatt
    19921 cttttatgaa gaaattttaa gatgattcaa aaagaaaaaa tttttggcta aaagtatttt
    19981 taaatgccac aataaaaaag accatgttct taacatccaa taaataagct caaaaaggga
    20041 agagaataag tatgaagcat tcgactttag taaaaaaatc aatagggcta attttcattt
    20101 gtttgtcctt tttattgttt tcctgcagtt cctacactcc taaagaggac tcagaaggta
    20161 acatgccagt taagaagcga tttggtggct atcatggcta tcatcaccac agacatgggc
    20221 attgacacca ttagaaatga cccaatgaac actcattatt agtgataatt aatgcctgat
    20281 atcgtatgaa aatattgagt atatgaaaac aagtactttt gttatgactt attaatcttc
    20341 cttatcctgc aagcattttt tacatgggtt tttatttcaa cggcattaag aatttcccac
    20401 ttctttatca agcattagat tagcccctta ctcattatgg tttatgaggc tgagttttta
    20461 gttattttcc tcttcaaact cacataagtt gagggcggga acaactcatt tggtgtgact
    20521 tctaatcctt taagaatttt tagaagattt aaaatggtta tattggcttc gccacgttcc
    20581 acagtcccgt agtaaccccg attcatttcg atgaaattgg caaaaccctc ttgagaaaaa
    20641 cctttctctt ttctgatttt gcgaatttgt ttgcctagta caattaagtc ggtttgtttt
    20701 ttcattcaaa aagcatatgt gctatgcttc atatatagcc acacggtatg tctatcattt
    20761 ttgagttttg tttaggaaat ggtaaaaata aaatcctatt ttgttatttt taacattgtt
    20821 aatttttaac acggtaaata atcgttattt gaactatatt gatacgatta ggagaagaaa
    20881 ataatacctg gctcactgga tagttatcag gaattaaagt aggttagagt tctttagtta
    20941 aattgagcgg actcatgagg aattcatctg taggtaaacc aattttagta ttgagaaatg
    21001 acgtgctact taattaaaag ggatttaaag atttgtttta tgctggagac catagatgaa
    21061 aggtatcgtt tttacctcct taaatgacat gattatagaa caatttggca tagaaacctg
    21121 ggaccaactc gtatcctcac tagaccttcc aagtggtgga agttatacag caggcggcac
    21181 ttactcggat acagaatttc agcaattgat tcaggccatt gcgaagagga ccaatcagca
    21241 cgcttctgtt tttttagagg cctttggtga atacatgttt cctatcttat caagtaagta
    21301 cgcaattttt ttaaaaaagg acatgacatt aaaagaattt ttaaaaagta ttgatggaac
    21361 aattcatgtg gaggtagaaa agttataccc ggatgaaacg ttacctacca ttagttatga
    21421 agagcccact gcaaaccaat tggttatggt gtatcgatcg catagaagac tctgtcattt
    21481 tgcaatgggg ctcatccagg gagcagcgca acattttaaa aagaaaatta ccattaagca
    21541 gactcactgc atgttaaaaa aagatgatca ttgtcgtttg gagattacct ttgagtgatg
    21601 agaacaaagc attatttgag cgctcttatt atcgcgaaag aaaagctcgc gaggaagccg
    21661 aattattatt ggaaaataag acaagggaac ttcatttact gaaccaggat ttggaaaaaa
    21721 agaattctga actgctaaaa acgctaaaag acttagaaag cgtacaaaag aaattatcga
    21781 aattagccca ttttgatgta ttaagccgat tacccaatcg attgcagttt gaactggatt
    21841 taaaacgtga gattgctagg tcaaaaagat acggtcgtca tctggctctg ttaagcatcg
    21901 atttggattt ttttaaaaat gtgaatgatc gctttggtca tgatgtcggt gatggtctat
    21961 tgcgtgaagt agcgaagcgt ctttgctcaa gtcttcgcga agaagattgt gtggctcgat
    22021 tgggaggaga tgagtttgca gtgattctga cagaaattaa taatccacaa caggcaaaat
    22081 tagtggccaa taatatgatt caaacgatgg gtcaaccatt ccttattaaa ggccataccg
    22141 tcatgattgg actaagtata ggaattgctt actttcctga tgctggtgag gaggcaataa
    22201 cccttcgtaa gaatgccgat attgctttat acaacgccaa agaatgtggt cgaaataatt
    22261 gccaaatctt cacaccgtct cttagaaaac aatattcaaa acgcttggga ataggaaccg
    22321 agttgcatct tgctttggag tgacaagaat tttttcttgt ttatcagccc cgggttgact
    22381 taaaaacgaa ttctatggtt ggaatggagg tcttattacg ttggcaacat cccaagcggg
    22441 gcgttgtttt tcctgatgaa ttcatttccg ttgcagagca ttttaacttg attattccaa
    22501 ttggtgaatg ggtcttacga accgcgtgta ggcagtatgt ggattggaaa aagagttacg
    22561 ctcctttcaa ctgcaccctg gcgattaata tttcgcttca tcaatttcaa cacaaagggt
    22621 ttatttcttc tgtgaagcac attttacaag aatatcaaat gccatcggat tggttagagc
    22681 ttgaaattac ggaagcaacg gcagtcaatt ttttgggaaa gattgaagat acattaagta
    22741 cgcttcgcaa tatgggggta aagcttgctt ttgataattt tggctcaggc tattcttttt
    22801 tatctcactt aaaaaattca ccagtccaat ccatcaaact gaaacaaacc ttaattgaag
    22861 atgctcatct cagggaaaat gataatttaa ttatcaaatc gacgattgct ttgtcgcaag
    22921 agttaggatt gaatgtggtt gtgggtggtg tcgaaacaga agaacaactc aattttttac
    22981 ggtcaagtca ctgtttcgaa gtgcaaggct attacagcag taaaccgtta acagtaggac
    23041 agatgacgca atttatcgag gaaaggaact ccaaagggct gtagctttat gttgtttcat
    23101 tcctatcaaa aaataacacc agttaggtta ccaaaaataa tttagcctaa aatggataag
    23161 ttaataagcc ccgctttatt gaccaaaatc gatgggttaa attttgtgct atgctgtatt
    23221 atatcgtgtt gattatagag cataatatca gggatgtcgg ccatgaaggg actagaaaaa
    23281 caatgctgtc ctaagaaaag aatactcact cagagtcaag aattgcaaga aggggtagat
    23341 ttctcgtgtt gtgcaccggc tgatagaggt acttccgata acttctttcc atttttcact
    23401 cgattagtgc aagccaactt ggctaaatgg acggcaggaa taagtcctgc tgccatcggt
    23461 tcttcttatt ccacctggct ttggcaattg gcccaatccc ctggggtttt gtgggaactt
    23521 gctttttatc cggtttttca tgccaaggat tgcattaata atatcgtttg tgttgagcgt
    23581 gctgcggatg gtaaagacgt gcgttttaaa aaagacagtt ggcagcctat gccttggcgt
    23641 ttattcgccg aagggttttt gcagatggaa gattggtggc gacgcgccac aacggacgtg
    23701 ccaggattac ccaaccaagt ggaacgtact gtttcattct gggcgcgcca atgccttgat
    23761 gccctatccc cttccaattt tgtttggtct aatcctgatt tatttcatga agccatgcgc
    23821 actgggggac ttaatctcat ccaaggcggc caaatcgcgc tcgaagattg gttagaaaag
    23881 ctaaccggtg caccacctac aggttctgaa cattttatcc cagggaaaca agtagctatt
    23941 actccagggc gggtggtctt tcaaaatcat ttaattgagc tcattcaata tgaagcccaa
    24001 acgaaaacag tctataaaga gccgatactc atcttaccag cctggattat gaaatattat
    24061 attctcgatt tatcatcaca taactccttg gtgaagtggc tcgtaagtca gggacacacc
    24121 gtatttatca tttcttggcg aaatcctgac aaagaggacc aggatttggg gatggatgat
    24181 tattatcggc aaggagctat ggctgcattt gatgcggtat cgactctttt tccagaaact
    24241 aaaatcaatc tgatggggta ttgtttagga ggtaccttgg ctatgattac ggcagctgcg
    24301 atgggcagag acaaggacga gcgtttaaac agcttgacac tcttggcagc ccaaggggat
    24361 tttactgagg ctggggaatt aatgctcttt gtaactgaaa gtcaagtgga ttttcttaaa
    24421 agtatgatgc gggaacaagg gtatctggat accaagcaaa tggctggttc ttttcagatg
    24481 ttgcgtgctt atgacttaat ttggtccaaa atggttcaag attacatgca tggaatgcga
    24541 cgagggatga ttgatttgac cgcttggaac gcagatgcca cccggatgcc ttacaaaatg
    24601 cacagtgaat accttgaaaa actcttctta aggaatgatt ttgccgaagg gcgttataca
    24661 gtggaaggaa agcctgtggc tgctgagaat attaagttac ctgtttttgc ggtgagcact
    24721 gaaaaagacc atgtagcacc ctggcaatcc gtctataaaa tccatcttat gactgaaggg
    24781 gatgtgacct ttgttctcac tggtggtggg cataatgcgg gcatcatcag tgagccgggt
    24841 cacccaggac gctcttatcg tgttcatgag cagaaacaag gcgaggctta cttaaatcct
    24901 gagagctggc ttgcgatggc agagaggcgg gaaggttcct ggtggcgaga atggaatgag
    24961 tggctagtcc agcaaaatac taaaaaacgc attgcatcat cggtcatgaa tccctcactg
    25021 ccagaggcac caggaactta tgtgcttcag aaataaaacg ctcttctttg aacaatagga
    25081 gcgctgtata ttagtaagcg tccgatccaa taaagccgca tccatttgct tagcaaatcc
    25141 tctaggtgga tggcggccac cctatcaatg tttaaaataa cagtgctcaa tggacttttg
    25201 agtgagttac atgtattgcc caccattaat atcaaaattg gcaccagtaa taaaaccact
    25261 ttgctcctct gcaaggaagg caacaacacg agcaatttct tggggtttgc ctaatcgtcc
    25321 cacaggaatt tgggcaacaa tcgcttctaa aatatcgtcc cttagagagg cgagcatctc
    25381 agtattgata tagccaggag agacggtatt cactgtaatg cctttttttg cgacttcttg
    25441 tgccagactt ttggtaaaac cgtataatgc ggatttagtg gccgcgtaat tacactggcc
    25501 gaattgacct ttgcgtccat tcacagaaga aatggtgata atacgtccat aactattatc
    25561 caacatattg gataacacgg ttttcgtcat attaaagaca ctattcaaat tggcattgat
    25621 gacatcctgc cattgttctt gagtcatttt tttcaaagtg gcatcgtgtg tcacaccggc
    25681 attgttcact agaatatcga ttttgccgta tttttcgatg accaagtcgg tcagtttctc
    25741 acaatcattg aaggaagaaa tatcggcata gacaatgtcg atatcaaatc cttgcatggc
    25801 ttgttttttc tgccattctt tcgctaagtc atgattgccc tgtttgaaat aacaggcgat
    25861 gacttgataa tctaactttt gcatttcttg gcagatggca gtacctatac caccagtacc
    25921 acctgtgact aaagcaactc gttggttcac ttccttcctc cttgatggtt ttatgcagaa
    25981 aaaatagttt tattgttatt aggatggtat tagcataagc ctaatggcgt tatttaatca
    26041 cttctacttc ttcagcctga ggtcctttct gtccttttcc cattttaaaa agaacggttg
    26101 ccccatcggg aagtgtttta aagccgtcac tttgaatcga actaaagtgg acaaagtagt
    26161 ctttgccact gctttcaata aaaccaaaac ccttgtcttt attaaaccat ttgactttac
    26221 cacgaatttt gttagtcatt ttttgtttat ttccttataa tcaagtgggt tatatagtgt
    26281 catgcgacgc tgttcttttt gtcaatactt gctaaccaga aagcttttag tggtttaatg
    26341 agtggaatca gtggcttttt cagtgttccc ctctactttt ttcaacgaaa ccactttatc
    26401 acattgaatc tcaatggtat ttgttttagt agcagatact ccccaacatc gtattctttt
    26461 tccctctttc tcgatgaata gcacgtgaat aaccaaattt ttcccataag tgttgtcgat
    26521 ggtgatgtct tgagtctcat tcgtctttgt caggagtctt cccagtgaat tgccattccg
    26581 gtggaactct attaatactt cagtattgga taaggatata ttatttattt tggagttctt
    26641 gtggtatttg atgtgatagg attgcgtgcc ggcgcaggca ttaagtgtcg tgcacgaaaa
    26701 aattaccgca aaaataattt taagaaggac tatttttttt attttaaatg acgtgatatc
    26761 gttcatggga ttcctattct tattcttgct catctatttt tactattttg tcataaaatt
    26821 tttacatgcc caatctttaa tagcataatt cctttttgtc ataatagagc tcagtcaaca
    26881 gaataactta ttatgaggtt ggagactcat tttagttttt attaacgact ctaacaagga
    26941 ttagtttagt atgcgtttac gtcttgtcat cttagtttta gccgcaatta ttattggtgt
    27001 gggaagttat tctgggatag tgaatgtcat taaagagccc atagaaaatg tgataatagg
    27061 gtataaaagc ccatcagcct ggaataatcg tggtgtagat tatgtgattg gcaaaagagt
    27121 agcacaaaat gactctgaag ctgtaaaatg gttttgcaag gcagcgaaac aaggcgaagt
    27181 catggcccag cgcaaccttg gcttgatgta tgctgcaggc aaaggggtcc ctcaggataa
    27241 tggcaaggct atgcaatggt ttcgtaaagc cgctcttcaa aatgatgccg tgtcacaatt
    27301 aaatcttggt gtcatgtatc aaaaaggtat gggtactcag caaaacgata gggaagcaat
    27361 caagtggatt cataaagcag ccgcacaagg atttccagag gctgaacgta gtcttggcat
    27421 cctatattcc attgctgaaa atgggcaaca aaattatgtc gaagctttta aatggcttca
    27481 caaggcagct gagaaagaag atgccatagc ccaatataat cttgcagtta tgtatgtcac
    27541 aggcaagggg gttcggcaaa atgatacgga agcagttaag tggtttcgta aagcaggcaa
    27601 acatggcgat ctcatggctc aacgaacact tggattaatg tacgcaacgg ggagcaatgt
    27661 tcaacaggat gattttcaag cgatgaaatg gtttcgtctg gccgcgaaac aaggagatgc
    27721 tgtggctcaa tataatatag gtatgggctt tcttaacggc aaaggggtga ttcgaaatca
    27781 cacaaaagct ctgaaatggt ttcatttagc tgctagtcaa ggattgcccc aagctcaata
    27841 tgtgcttgct gcactttatc atgatggagt gagtcttcct caaaatagca tggaagcaat
    27901 aaagtggctc cgaaaggctg ctgcgcaagg tcacttgcaa gctcaagagc gcctgcaggc
    27961 tctggttaga caggattagc tgtgaggcgg ttcctctttc attatcatac gagttagtat
    28021 ttatccccct atgctgaaaa taacgatttg attatttcaa tgagaaatta tttaaacata
    28081 ataatatttt cgttcacgat tttgtaatgc ttatttaatt gtaatgggat aagcacagct
    28141 cgtcatttca gagattaaca cctttacagt ctgagttgtt attttctatc taggttctag
    28201 atataagccg tttaaatttc tgaaaattca tttaggttaa aattgataca cctaaaagtt
    28261 agaatctgcc tcatttattg ggaatatttt tgcaacacta tcattaagta atctgagcgg
    28321 tacttttatc cgaagcacat tgccatgcga tgttattatt ttagccttta agtgtcgtta
    28381 aagagcatct cttttcaggt tttatggttc tgtaaattat tattataaac attgttgcta
    28441 ttagcgtaaa aactaaaatt gatggtgacg atgcaacgcc ggtttttttt acaagtatca
    28501 tagaaagtaa tggagcgagg ccagcggata aacttgctgc aatggaatat agtattgata
    28561 aaacactaca acgtaattta atcgggaaca tttcagctaa cattactggt actgttgcat
    28621 aatatgctcc tgctggaata gcaattaaag cttgactcaa aattaactga taaaatattt
    28681 tgtgcgataa gaagttgaaa aaaggttgag atagaactag aaatcctata cttgctgcaa
    28741 ctacgatttt ttctctacta attttatcag atagattccc aatcagagga aatactccta
    28801 gaagtaccat taaagaaatg atgttggaaa tgataatttc atggttttga aaattaccat
    28861 ataaacgagc ttgcatagga ccataaatgt aaatttgaaa tgtagttgtt acacccaaac
    28921 aacttaaaat aaacacataa agagattgta ttttattgtt tttgatatag gttaccgatt
    28981 cagaaagtaa actcttaaat ttaggctttg gtctgtctgc atagtatacg atgtattcca
    29041 tactctcggg gatacataaa cggatgtata aaccaatcga agatcctaaa agcgcaagca
    29101 caaaaggaac acgccagatc atccagtcct gttcaggata gtgactggta aaataagtaa
    29161 caatcaatac aactaaagac gctatcagca tccctgacat agcgccaaag gatgcccagc
    29221 ttcctgagta gccctttttt gaggcttcgg cattctcaac taggtaagca gaggagttta
    29281 aatattctgc accacaggag aagctctgaa taattcttaa cactaataag gtaatagcag
    29341 aaaatgtacc tatagtactg tgagggggga taaacccgat aagggttgtt gatatgccca
    29401 ttgttaggat agaacctgca aggataattt ttctgccata gatatcggat agtaagccca
    29461 ttatcatagc cccaataggt ctggctaggt atccagcaaa gaaagtaaga aagacgagaa
    29521 acaaaccctt ggagacatca ccaatcaggt atttggcaat gtaaactgag agtaggccac
    29581 aaatagccat gtcaaaggat tcaattaaat tacccagtgt acctgccaga atgattcggg
    29641 tttggtgctt catgcgatta tcctttactt tgcattgccg atagagtggt tagtgaatct
    29701 tgatcttcca ctcggtctaa aaataaaaat ccatccaggt gatcaatttc atgttgaaga
    29761 atgcgggctt ctaaaccgga agcttttttg gtaattctat ttccatctat gtcaaaccct
    29821 gaatattcaa tttccatggc tcttggaact tcacccatta attcaccaca attaaggcaa
    29881 ccctcgtagc cagtttgaat ttcttgagat aggattttta atgaaggatt aattaaggct
    29941 gtatcaggaa ttgggtattc tggttttctc cttttggtgt aatcagtacc aaatacaata
    30001 actcgtttgc tgatgccaat ttggggggca gctacgccta cagccccctt gtcagccatt
    30061 atgccaaaca tagtcttaat aagctctttt aaccaactgc taccaaattc tgattcagaa
    30121 attggatcag cggtttgtct taaaataggg ttgtttttat caaggagtgt gttcatgggg
    30181 cacctgattg ttgatgatta atgtcagttt gggttttgtc gtcatgcagg gctgtttgtc
    30241 agggaaaatt aaatactctt gaattcgcct taacgcaaat ttgcccatgg ctaacagttt
    30301 ttcgcaatga atcgagcttt tgttctggat atatggattt atccgtttta atccaaatga
    30361 aaagatagct ttataagaat catattcagt ggggatggag atacctgtta taagtctagg
    30421 ttttatttgt ttgtatttta caattgattc tgtgcagcct atatgtggta gttcgctcca
    30481 taatttcggt tgatcctggc tgaggaattc tggttgatag caaccgtcaa atagccagag
    30541 actttgctca attaaatttt gctctatggc tgggttcgag gatatgaaaa agatctgtcc
    30601 tgcctggtta ataagggcaa tagaagcata gtcagtttct acttgcccta gtacatctga
    30661 aaacaccctg cgtattgtga caaagtgttc aaacagaaat tccttggctt tggggtgaag
    30721 ctttacttcc acagctgtta catccatatc tacaagtttt ttaaactgca tgactgtgct
    30781 tttttctctt ttcttcataa gaagaaattg aaccaatggc caagtcaatg atttttagta
    30841 aattattttc cgaagtgtca attgctttga cttctttgat caatcctaac acaaaatcgg
    30901 cgtaatcgtc attacttccc tgagagatag ggtataaatg atggctttgt ttcaagatgt
    30961 aatgaagcaa tttttcatcg acttcattct tcttcgttgt tgcattttcg tcttccccaa
    31021 atagtaacca gcctgggttt acttgaagat ggaaagccag ttgtttgact ttttcatagt
    31081 ctggtaaagc atctcctcgt atataacggc ggcagatttg ttcagaggca ccagtaaact
    31141 cagccagggt tttaatacag atcccatttg gcgagcgaga ggccgtatat cctctatctt
    31201 tgagggcgct aatcaagcgg ttagcaaacg attgataggg tcccttactc ataaaattat
    31261 ccttgtaatc gaacgtcata aaaaattatc atataaaacg atatcattca atatcatatc
    31321 gtatcttaat ataaatttct ttaaaacaca tattgataat aaaattaata ttgttttaat
    31381 ataagtttcg tcaaatgatt ctaaatgtta atttgacgtg aattggactt tagtacacat
    31441 gatttacagg agtatttttc atgtcccata ataataaaat aggtatttac aaggggatta
    31501 tccagtattt gctagatgca actaactatc cgttgcagcg aattgctaac ttatctaact
    31561 caccaattgc atatttgcaa ttgatttatc accataaccg attgcttcaa aatagaagtg
    31621 tagagctaaa tttgctgaaa ttattcattg tgtttatcga tatggaaatt aaacgagagc
    31681 agaaagctga gaagcttttt ccagctgaca tgcattagga ggctttaaga tgcagttttc
    31741 tgataataaa atgccaaaac agattttgtt tattactgat ttagaggagc ttcttggtcg
    31801 taaccgtttg acattaaggc gttggtggac tagcggaaaa tttccacaac cagttaaact
    31861 aaatggcaca acactggcat ggcactctga gacagttaat cattggattc aacaaaacat
    31921 gcgattatct tagcataata gcccatatta tgtgggctat tgaacttctt tccacaatgg
    31981 atgatctaac cagctccttg gaattcccat gctttcatgc gcccaatcat atttttcaat
    32041 aataggctga ataagtttct cggatgatat tgcccaatct ttgccattac ccgcagtggt
    32101 tagtaatttt ctgatgatca acaagataat atatgagcga tcatttcttg gtgtaatagg
    32161 aggtagccaa agttcattaa tcgcatcaat tttgggttta atggctaaat ctttattcca
    32221 aaggcggcta tgatgagcac atatatttct tacataagtt agaaagtata gccaattggc
    32281 taaagttttt ggatgtaact tataaccctc agcaattatc cttttgtctt catttttaag
    32341 ccctttaaat aagaccgaca atgcaccaaa ggataaaacc tctattgcca tccatattgg
    32401 taagttagga tagcccagat atttattttt ataatgctca ataaagtact ctctggaacg
    32461 ctctatctcg ttattaattt gtgctaacca ggtattatgt tcaaattgct gatggaaatt
    32521 ttgaggattc gacaaagcaa atggtccata actatgagca aggtgataag caatactggt
    32581 tcttactgaa atttcaattc gctctaatcc atccattact aacaatctta gtttacgatc
    32641 aaactcatag agatctatga cattttcaaa tgtaacatta tcttgaaatt gttcggtgat
    32701 atttcctgtt gtatctgaac gtttaaaggg taaacagtag gcactaagcc gataatagtt
    32761 ggtattagaa aggctttgca gggctgaagg ccaattattt ataataagcc cttttgcttg
    32821 aagtttttcc agttgatctt ggaaactcag ggctggcttt agatatttaa tgagaggatt
    32881 attcatagca atacgcataa aaaaaccgcc aaggttacgc aaggactaac atcctctagg
    32941 cgtggcggtt gtgttaagtt aattatagag cagttcatat gattttttca atcctttgat
    33001 gagattaatc ttgcaatatg gtttgcccag gaatccaatg cctctttgcg ttggggtagc
    33061 atctcatttt tattataagt agccataata cgaggcattt tgtggccaag acatttttct
    33121 atcaccacgg gatcaatatt tagtgactca cccaattgtg tcgcaaaggt tcttcttaag
    33181 tcatgggcag tccattcagg aattcccacc cgattctgaa ttcttttaat tgcgcgtgga
    33241 agggcatctt tggacatagg aacatcgatt gttatgcctg gaattacaaa aggggagaca
    33301 gattgttctt taaactgaag tagtaatttt tttgtaagtt cactcaagtg tatttttaca
    33361 gttattcctc cttttgaatg ctcgggagga atagtccata aagattgctc aaaatcaaat
    33421 tgatgccatt gtgccagtcg gatttcacct gttctaacac cagtaagtat aataacttta
    33481 attgctaacc tggctgattc agacatttgg cttttatcgc tttcaaggaa tttccatatt
    33541 attcttattt cttcgggcga tagtacccgc tccctgggct tttcaatacc accgatatca
    33601 cgtgatcgta tattggtagc tggattatat tgaatataac cacgactcac tgcatagttt
    33661 agaacttgtt taatagtgct gagaatgcga ttcgcatgaa ttggcgctcc tcgttgtact
    33721 attttgtcaa gggcgattgt aatatctttg gtttgtaact tatcaagtac agtatcgcct
    33781 agtagaggaa ttatttctga gtcaatctga tatttgatag ggcgtggttg ctttctattt
    33841 ttaacggcat aattttcgta ccaagactgt attaattttg aaaaggtatt gtcttctttt
    33901 aatttttcta gcttggtatt ttctatagga tttatacctt gctcttttaa actttgcgct
    33961 ttagcaagat attcacgagc ctgttttaga ctaatagctg gatattttcc taaggtcatt
    34021 ttttgacgtt taccattcat actaaaacgg taaaaccaag atttattacc acttggcatt
    34081 actctaatac ctaatccaga agattctatt ttttcaaacc aggtggtctc tggcgcgaga
    34141 tttttaatat agctatcgat aaatttcatc tacacagtcc tttttatttt ggtaccattt
    34201 ttggtaccaa aatttatgta taggtatatt atcaaatgat actaattgaa ttgctaaaac
    34261 tcaattattt cttataaata aagggttttt tgctattttt gaaattaaat gatattgttt
    34321 gatatcggta taataaagtt tcgtaatcaa gaggtcggag gttcgactcc tctcagcggc
    34381 atagtaaaat caatgggtta gtaaaactta tattttaatt tctataacgt gtcgaatatg
    34441 tgtcgaacat tcatgaagct ataatat
//
LOCUS       DACTJE010000016        33345 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.016, whole genome shotgun sequence.
ACCESSION   DACTJE010000016 DACTJE010000000
VERSION     DACTJE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 33345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33345
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.016"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            202..942
                     /gene="ravK"
                     /locus_tag="I9760_000559"
     CDS             202..942
                     /gene="ravK"
                     /locus_tag="I9760_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavK"
                     /protein_id="HAT5042350.1"
                     /translation="MVSLEHIQKLISECRKLGKDGLDNGTNGLIPELEIDVVPPSAFL
                     GVGNNPAIFVNSKTYKLMRTTHEKWVENKTIVFKSYLLSQPAIKIIGAIVHETGHAFN
                     VAAKIPNTEANACIFEIEVLMRLFQLKSPLLLGCTELDMQSYFKSRLTDYNKCVKDCR
                     CLAEMVQLITHQFKLDEVSISEKENQIPLLSISNKWPGLFAKKQIAPDMDKLLTSPVT
                     ITPEVKILFYQLVKEHFHSPETEIKLDI"
     gene            complement(1045..2508)
                     /locus_tag="I9760_000560"
     CDS             complement(1045..2508)
                     /locus_tag="I9760_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT5042351.1"
                     /translation="MKTNIPTLSKPIKDSGYRRGLKDRHVQLIALGGIIGSGYFLGTG
                     EVINLVGPSVFIAYLLGGLIIYLTMLCMGELAVAIPISGSFVTYTSDFISPTVACGVG
                     WSYWITWVAYIPAECVAGGIIMELFTGVSGYIWVVCFGLIITYINLAKVDTFGEIEFW
                     LALIKIISLLAFVFLAILIFFGLIHGSEPPGIIGFKYLLGDGGLLPNGAMSLLTAMVL
                     LLVNYQGSEIIGLAAGESENPARMIPHAIRNVTFRILFLYIIPVFCLVLIFPWQKAGL
                     SNSVFADALNFYGLKWAGAVTSFVTLSATLSCANSGFYGAVRSLNALARDGMAPHVLA
                     KFNQNSVPQNAVIATLIGVWILLGVGYFFGQTKLYIALLLVSGFTGTLAWLSLCIAQI
                     SFRNRLYKAGYSIKDLRYVTPYSPYTGILAVILMVGSLFFLLLNKDPIYKLSFIIGVV
                     SFIIPVIIYKVFDLSKVRKKALHVKSRVKFQDLFPPQ"
     gene            2889..4034
                     /locus_tag="I9760_000561"
     CDS             2889..4034
                     /locus_tag="I9760_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HAT5042352.1"
                     /translation="MLRWIVLICISLFLPLSAYADKSDCINHHCIAVIDAGSSSSRLH
                     IYAYDLDETNTPIAIREIWNRKAVPGLSTIEHENKSVSAYMEKLFSDAPAVLMPVYFY
                     STAGMRLLPKTHHRNVNQLVAEWFDRQYYWRLVTARTISGTEEGIFAWLATNYQLNLF
                     QQEPQTLAGVMDIGGASVQIVIPVVNPSFMDHEHTTTIRLYGKEFTVFSHSFLGLGQN
                     EMGHQFFDLKFCFPEEYELPSGRQARGDAYACKDEIAILVNSVHSVSRKIKSVLAANP
                     IEKWYILGGITFMLNDKLFSLDNQYFTNRDLISFADQSICHQKWSTLEELYPDNLMVF
                     NYCMLPSYLYALLVHGYGLKSYQKVNFMPNSKNIDWTLGVVLQHKQS"
     gene            4051..4123
                     /locus_tag="I9760_000562"
     tRNA            4051..4123
                     /locus_tag="I9760_000562"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4084..4086,aa:Thr,seq:cgt)
     gene            4593..5351
                     /locus_tag="I9760_000563"
     CDS             4593..5351
                     /locus_tag="I9760_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HAT5042353.1"
                     /translation="MKLVFNNDVLAVIDDFLEQPSFEKIWNFIQTEKFKFVHSSKWVN
                     AFSLEDGSPLWSSVTISHPRPEACTTEKVYPTNSTIDLFINELIARSSDYSHLIGFKD
                     RDWDFFYARPYLYPRGSGLSWHTDGKYKISGAYVYYCHPIWDINWGAELLINPTPQLD
                     FDYPEVTLINDKKKKVGYHLNSAELNKYADDGVGYYIVPKPNRLVIFKNNILHCIKKV
                     EKAAGNHVRASITGFFMSNEKIHETKEAEKEHSI"
     gene            5421..6500
                     /locus_tag="I9760_000564"
     CDS             5421..6500
                     /locus_tag="I9760_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042354.1"
                     /translation="MNLVHQITPTLAQEKVNFVISIPHSDEAALSNFKLRVEDIPYNE
                     QPSHYQFKKWSSWCSAHFSSYYYYIKYKNALLRCKQFLYDFGPLSTLDHAALYDHWVW
                     EMKPHVLNQENICWIGYDYSKSKTLSFLSHGTNIELRLLEGNLSDEEFLELCKFFSPL
                     SSSSEILEKSFFELSYWSRYRNCIENACINRNEYKPPSSIWNLRWPMHNIFRGSKPDC
                     YIKYSNQLNLDFIPDSQIIYGNENQIEEIQFVLFPKNGKKNQMAWFRVFNKASFPIKK
                     PALSRLPSMNSFSGYSNFNLTVLSPKSSMIASNIYIASLNNCYGPHDALWWDNENAYL
                     LQLSASANNSIAYLKGILCQLIEDK"
     gene            complement(6520..7353)
                     /locus_tag="I9760_000565"
     CDS             complement(6520..7353)
                     /locus_tag="I9760_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAT5042355.1"
                     /translation="MGSGVFIILSAILHAAWNTFAKRNSTPYYEVLGIMSFATIFSLV
                     FIPFFSGPLFKTSDSFLWALTSGLFEGGYIITLAICLSQYSLGKAYIIMRGSAMIIVW
                     FISACFLGENINLSNIIGVSSVLFGLILTSQTFNPDQSRQLLFPVLCGICIAGYHLCY
                     GRSLAYGANPAALFSAALWIALPCVYLLMNKEQKQVFKEQIKSNWKITSLGGIISALS
                     FLLFLIGLRHSEAGLALTLRNTSVVFAQIFAYFIGEKISQYQWIGAFLVALGASLIYP
                     Y"
     gene            complement(7493..7999)
                     /locus_tag="I9760_000566"
     CDS             complement(7493..7999)
                     /locus_tag="I9760_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAT5042356.1"
                     /translation="MDTPSIYVACLASYNSAILHGVWIDATQSEDDIMEEIWEMLDNS
                     SEPNAEEYAIHDYEGFGSIKIHEYESISNIVEYASFIKEHGELGLALLCDYSVDDAQT
                     MLEDHYQGCYDSEVDFSRQLFDDCYAHQMPDNLICYFDFEAFARDLFISDYCSVEFGG
                     QAYIFANY"
     gene            complement(8065..8487)
                     /locus_tag="I9760_000567"
     CDS             complement(8065..8487)
                     /locus_tag="I9760_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAT5042357.1"
                     /translation="MTASLNQVQLIGNLGADPKNITGKDGQSFVTATLATNESFKKND
                     EWQTRVEWHQLVLFGKLTKVAEYLKKGSQVYLEGKLRSNQWTDNEGKTHQTLSVVVNH
                     IQLLSHFKSADESSNKTAEHHMVQMREMLQTDSEEIPF"
     gene            complement(8588..8782)
                     /locus_tag="I9760_000568"
     CDS             complement(8588..8782)
                     /locus_tag="I9760_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042358.1"
                     /translation="MQQQEPAQLEIEHLLFELNCCVNDLSRHRLQINPYDIQQAELSL
                     HKLESLISFVKQFDTQRIAC"
     gene            9196..9441
                     /locus_tag="I9760_000569"
     CDS             9196..9441
                     /locus_tag="I9760_000569"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042359.1"
                     /translation="MSDHRKTRLAFYFLCEKEACSESFSLDELEQAAEWSASTVDTYL
                     SKKWKHIVSRSADGLYTCAGICKMSLNEFVNLQKQTA"
     gene            complement(9515..10414)
                     /locus_tag="I9760_000570"
     CDS             complement(9515..10414)
                     /locus_tag="I9760_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAT5042360.1"
                     /translation="MSKSKLKSALFSINECIKELSVYSHASKADTRHILNRCIKDLHE
                     QGFMVAHIKGLKSKHIHVLVEYWKKQGKNPATIKNYMSKLRKLSIWLNKPEIIKPSND
                     YYQIDNRNYVPTYNKAITNIDFSKCENPMIRLSLEAQYLFGLRREESMKLVISEAWFG
                     ERLILKSSWTKGGIGRAIDITSKEQKEWLEKTIKQISPGHSLIPKNKTYKQYLRQYQT
                     ETEKLGLKNCHGLRHAYAQRRYHDLTRQLSPDKQPFKCPIEGGKTSKYLSQSEKAIDR
                     EAREIISRDLGHSRISITKSYLG"
     gene            complement(10407..11210)
                     /locus_tag="I9760_000571"
     CDS             complement(10407..11210)
                     /locus_tag="I9760_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAT5042361.1"
                     /translation="MRKNSLRQAANRYFQLDNRGSYQEKKKRAYVIQKMIDDLFRIGD
                     VPTSWQNMKSEHIQKLVGYWKRNHVKDATIMRYMTIIRNYLLSINCPIQNIDNKSLQL
                     HRKILKPQRLKWQPNLWESFSEMTSRIIMALQTEFGLTFSEAIQIKPGIHIKEHKLSL
                     TREITFNSVDRTIPFRNEIQKNVVSDLEKLTNRQSLVQFFGYKQVKLSWNHTLMAFDV
                     PVKKSWRYLYAKQMYQYLLPILGNYQTCLILQDELGIKSRNTLWTYLHE"
     gene            11501..11704
                     /locus_tag="I9760_000572"
     CDS             11501..11704
                     /locus_tag="I9760_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042362.1"
                     /translation="MIFPTERPATELGNKVALHKTLDYVSLESRQLFSCASLKNIKVV
                     LAVQGLKENNFVLTPVWRQDKIQ"
     gene            11856..13190
                     /locus_tag="I9760_000573"
     CDS             11856..13190
                     /locus_tag="I9760_000573"
                     /inference="COORDINATES: protein motif:HMM:NF014069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAT5042363.1"
                     /translation="MKKSQLSRNHIITDPIHGVMSFSKDEKELIKQFIDKPIFQRLKR
                     IKQLGCADMVFPGAVHTRFSHSLGACYIGKLVCDQLDINNGDRKIIMGAALLHDIGHG
                     PFSHAFESIFKGLGEGKISHDKQWTPKFIDSFECDLKEDILNVFIDPNHHEYYDPLIA
                     SQLDVDRLDYLLRDSHFCGVPYGNIDLQWIISCMKQQEINGDTVICITPKGIGAIEHY
                     ILSRRLMTKYIYYHGKKNSSEYLISTFLKNLEGHLDIAELAQSPLFLFMKNYFEYKRI
                     INKNTDNNESNRNDFVDNSFNYYKEITDDDIWIVLRRLSNEDSIAGRVAHKICNRELP
                     NSFLINPSRRFDAKKIIERYKDQEDEDNKWRIYFDELNFESYGSKKIPIYVSDHNTVT
                     DLYHSSNMLNQISDKPEPTYYVYIDAKHPKEEELLCELSSYHCLVSPYFKSG"
     gene            complement(13246..13515)
                     /locus_tag="I9760_000574"
     CDS             complement(13246..13515)
                     /locus_tag="I9760_000574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042364.1"
                     /translation="MKTTKYSSNLINQLPNNKPVVYEIQTAGGNKNYIGTAKRGRVTG
                     RIAEHIGTIPGAQVKIKQFSSIQEARKSEAKQISKFQPKYNKQGK"
     gene            complement(13616..15601)
                     /gene="traD"
                     /locus_tag="I9760_000575"
     CDS             complement(13616..15601)
                     /gene="traD"
                     /locus_tag="I9760_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HAT5042365.1"
                     /translation="MSNYPINNLLREPTEAWSAITCLSLALLAYTQPHLFLLTQTMGL
                     YAALALLIPGIYRAWQAIKVKRYHRRLLAMPTYALSTTEIPLSKNWLFLGKGFRWRPS
                     HTQKLHQIKQVKNEKFMQRSHCYQLARRFCQNHEHTKLAKWLNTNSKLNPFRPDPPVG
                     GSPFLHGVGEEDKPVYIPQDVRVGHTFVVGTTRVGKTRLASILINQDIRNGDAVIVVD
                     PKGDQDLVRDMIAACKVSGRIEDFKIVHLGFPEQSAQYNPLKNFDQISEVATRITDAI
                     SAEGEGKQFAAFAWKYVNIVAICLEEMNQPITYQTIAFYISRLEQLLMSYADKILPNH
                     YTNYHQEIDEIIEEHDSKIGKNNKTKSPMERHQAVIQYVKDHINKTIKSNNAEALHDQ
                     ILIDLYDAAIMDKHYYDKITASVGPVLSEINKSNASKILSSKAQPGDIELMDVIKNKK
                     VLYIGLDSLTNPNIAQAVGKAFLSDLVSTAGKIYKEPNARYTLNLHCDELSEIIQDSF
                     VKILNKAGGAGFQVTAYAQTIQDMEVALGSRAKAEVSEGNFNTLIMLRIKNEETANLL
                     VKVLPQVGVVGHTQVSMVNDTPHGDDGVYFNTTNEDRVQTSSVPMIGVDDIISLPKGQ
                     AFILINGGELFKIRIPLPNNTQYLTSKSPISLQANAI"
     gene            complement(15598..15927)
                     /locus_tag="I9760_000576"
     CDS             complement(15598..15927)
                     /locus_tag="I9760_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042366.1"
                     /translation="MIKHYIRSSIIVLIQTVLPMIAILVIAPWFINSNLLARWQSTFT
                     TIQPLFLVLHGVLYLALVLLWSKLISRLQNQHQLTAEQLNTALNARWYLLAIFVFIDA
                     LMIGSRL"
     gene            complement(16121..17674)
                     /locus_tag="I9760_000577"
     CDS             complement(16121..17674)
                     /locus_tag="I9760_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HAT5042367.1"
                     /translation="MIVFNPLSLYTTYLGWQQYEVLFNALWQTGLLYLGFLAIGYRFL
                     KNVLNPAGAFYAVEHALNNFLYELAITFLICSLFVYPCVPLETKALQFKPLCGLKNPT
                     TAVIGDSGTTYDEAFADLLTNQVKIPIGFAIIQNFISSFTYSLMKVTGCTDSLQSIQG
                     DLVSTYLPQSVRKQALDFHRQCFIEARTQFNSEKHEASELDPMLKRYGGEDDLNWMGS
                     KILQKMYYSKLHARQPVPGFTFHQAPNRNLEKAANRGDIPPERLPEDGYPSCQQWWNK
                     IKTDLVEVSNQASVFNKHLNYYAMLDRVRQYKVKHPKAWKADISAEDFIAKMLLQESR
                     DMQTSSVQNLMDNNNGKVASAITHSLVNVGQWTKSWTSTPLKREAIMQTLPVMQAFFM
                     FFLIILTPMVLSLSCYSTRALGSLCALFVMAIFLQYLWHLVGFLERSVLDPLGENDAI
                     SAMKNMAVMFYFVAPVLLLRLSSHFGGDAGAGLMDLVNGSDRQSESMAQSGMQAAKTG
                     VKIIAKGIR"
     gene            complement(17677..19044)
                     /locus_tag="I9760_000578"
     CDS             complement(17677..19044)
                     /locus_tag="I9760_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HAT5042368.1"
                     /translation="MNKYLLLTLLIPHLTHAGNFMPSQSDYYYELGGTSNLFIPPVNK
                     DQTLVIGADIDGRMGFSCSGFNPVVSITNTFQDLKKSALNIPGGVIDNLKGSVAGFPL
                     YKLQQSMPALYNVLQNAASSAQNEFTLKVKDCQEVKQALEQNQSPMEGILSVSDSQGW
                     LDAAKRAKKENVDVTETSKSIAQKRDEYGLPWIGSNKGNAGGKFQRPIKVINDVVIAG
                     YNILLERKPLDSLEKPSTKIPMVQSWPTPTDAANWAVKVLGDIQVSSSENKQSHDAKA
                     GIGLSALLQSCANANTCSQNIAKALWNLVNGTWTLTEENLSKVSASNLLITDEVITTI
                     QHLPREEQMLTVSKLAEEIAVQNMLDKALMMRRILQAGLQVQEVQNLKPAMNMVLFAL
                     KKLDDDIHSLSFENDVRKKMMTETLGTIMDIRHQAQSQNMPGQDHEQPAVKNGAIYVK
                     ENKGA"
     gene            complement(19054..20028)
                     /locus_tag="I9760_000579"
     CDS             complement(19054..20028)
                     /locus_tag="I9760_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HAT5042369.1"
                     /translation="MTNTALALESTSPPHPVNTFTIATRVLQKLFQNSHYKVIGSCTW
                     TVGRLPPRLEVTPAVEQFLPDLVITVSNKPEENPWLEARTLYENKAARTMYQQAYKAA
                     TGSALGFGNDSGQTTDLHINDERTRIVDVIGSPAAFYRIPYLSHRPETGFGVPYYLAE
                     ADAVMDRTEAAELLYMGTHPHLLINHEIGTLTQHWGSEIPRLMRVTQPYNYRASVVTA
                     MHAVDIVTNKNPLHVTKSTNNSCGKNCVVANAIYDPQNNKVIWQEIYPLNRNIHPGDA
                     QDFGIEDEKAGNGNYVFVLWRKYRGCIQNKGKLVNFLTFPKVGQPQKR"
     gene            complement(20021..22771)
                     /locus_tag="I9760_000580"
     CDS             complement(20021..22771)
                     /locus_tag="I9760_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HAT5042370.1"
                     /translation="MKRLSNWLLGEMNSQAIKEQTIKKQYANPLPSFADRLAIVDFNE
                     QEQVFLFADGKSLGSGFELGDIQAEAASPEYLQAVFNKIRDTFATVVPLHAIDPWVMQ
                     MFVQDEYCLDPVIDHIKSKIPQQLVENPLTQDYLQRLQDLYNKMTRPEGLFLDPKTGA
                     PYRGRRRRIRVLFYRQLHQTTLTREQILLEHQEVISQIETKLRSPGLEIKRLKGQDYY
                     QWWIRWFNPKSADEILEQYPYPNPMPAGFNLTQNIFFSPPESDEQGFIFEGRKQRILY
                     VDGLKEAPIIGLVSRERQQANPKHRYALLDTLPEGSIYTIQVVFSHDENLDSHLTRLE
                     KGIVGTSLKPQEVRDDIKTARNELSTGNRLFWVNQAVFYQAEDEQQAQVIEKNLHTIF
                     NDAKMPLIHSSYDIHPLNSWLNALPFNFDPHYARKYLCFDRLMFATELAALLPVYGRN
                     QGARHLPCFPCFNRLGEPVFFDLLHHDFISQNSHCAIFANSGGGKSVLTGWMIQSLLA
                     MKNARVVLFEMGNSFDRMLVHAKAHGKKTKQLLLSNKKEEAVPLNPFCEAYKAIPEIT
                     DNLSTEQAALIAQKIMDLKDPKHSEDLACGDGSRAYLAELALALRTMITEANAMEEEQ
                     FTLADETLLIEVLIDAILTSFNDGIPQMLTEHIVSSFQRRLDKETVARKKDRILDMHD
                     RLNSYVINSAKSRFFNVPTEPLGDFDIFHIDISAIKDDTGKLALVMVSLLPRILAMAE
                     ATQNDNRPTFLFIDESHLQFQIPSVVTVCLLVAKVARKLGLWLVAITQNVTDMNSEKS
                     AKILSLIETWILLGLDEKEITDVKRFKSLTPQQETLIRDIDSQKGLYAEAVLLGSRYQ
                     GLFRVIPPRYLLALLMTEKSEKAQRHLLEKEHDVLVAAEIMAKKLEQPKATTHNRAYF
                     YD"
     gene            complement(22781..23128)
                     /locus_tag="I9760_000581"
     CDS             complement(22781..23128)
                     /locus_tag="I9760_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HAT5042371.1"
                     /translation="MVTINKRTLTMLCASLALSACSTANISQSAIPEQGLTVSQLYQQ
                     SMQQPIQKAVVQQVRFEDSGEEFKQLPNPEVPIHIFAHVAQLGDEQIIKPAYTTRFFL
                     YKQNQYALASELY"
     gene            complement(23085..24347)
                     /locus_tag="I9760_000582"
     CDS             complement(23085..24347)
                     /locus_tag="I9760_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HAT5042372.1"
                     /translation="MNKNKGIKILAGSVVFAVVMILINSRHSTPIHDDTTPNPNNFHE
                     AITSQFNDNIRDVSARLLETEKKLEQMHKENKSLQKQLKQPTQEASHTLQDELQTLKE
                     KLSALTDHQTQSYPMEGTQASVSGQIKDLDGLLDKAPIESKIFADQDSSTKEEAPTKT
                     PFYTIPAGSDFSKVTLLSALMGEVPVEGKLMQPLFPFTAMVSRGDLMTANGMQLPEEI
                     IGMKISGYSIGVGSFLDNISCVRAYITAALFVFEDGHFVSIGQEQMKSSAELVNNDSI
                     GYLTTQFGNPCIKGQYITNAPRVLAAFMAAGGIQGAGNALSKWQMSYQAEGGSAIATP
                     TGDLAHFAAGGAINEGSQKITDWLEKRIQGSFDMVFVPASIRSHSGFIPNQFSLHFSQ
                     TIAIDKEHQGRVLDYGYHQQKNFDNALR"
     gene            complement(24344..25120)
                     /locus_tag="I9760_000583"
     CDS             complement(24344..25120)
                     /locus_tag="I9760_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HAT5042373.1"
                     /translation="MKLIKHILLIIGFMLTQVSYALQSEHLVWEKVPLTIELPINKER
                     LVQFPQAIKIIDQQLSTNLDILKVKGSLYLKAKDAFNDARLIVQLLPEGEVIILNLKA
                     NEKAINTTPIEIVIDDPQATHQSGASQYEYNAIQLTRFAIQALYSPERVRDIPEGIYR
                     SPMQTNKTIPLFYGASIEAHPLASWRGGSLYITAVDLKNLLNQPVKLQFSKLMGHWQT
                     ASFYPKGELPPRNQHESTTVFLVSDQPFATTLTQHARYSR"
     gene            complement(25107..25763)
                     /locus_tag="I9760_000584"
     CDS             complement(25107..25763)
                     /locus_tag="I9760_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03746 family integrating conjugative element
                     protein"
                     /protein_id="HAT5042374.1"
                     /translation="MFEYWKKIDSLQHHNRLLLAFIGLLSAIVLALIINLMTMPKRYE
                     FWLSPNMAANGGLMKASDIPNEYVQGFVSSLIPTLYTWSNKGKEEFNQNIKAFHYYFT
                     PRHEQLLRDTLGAYKNAQLFDRSQVASLYRFMEPQDIRKIGPDTWEVKLILRITQRLK
                     DNSGMVIADKVVAYHLRVVKLNLSRLHNPFQLALDGYTRPEERLQDLLVDTEVSHETH
                     "
     gene            complement(25756..26118)
                     /locus_tag="I9760_000585"
     CDS             complement(25756..26118)
                     /locus_tag="I9760_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HAT5042375.1"
                     /translation="MSQPSSRHLSHDFPAWKGLSLRELFWIVITTTPVTTLFFILLGT
                     MAGYPLAAGCIGFVIGFILSITVWPKGVARIKAGKPYGYVMKKTILWMVRLRLKHSPW
                     IHYQGQWQKNKSVGEPHV"
     gene            complement(26130..26495)
                     /locus_tag="I9760_000586"
     CDS             complement(26130..26495)
                     /locus_tag="I9760_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042376.1"
                     /translation="MKTTLRTLYLGAVNLFYAPVLLADNWFPKISDADNIGDGSKSMM
                     SVTGNYVKQGLGLLLFITAVVTFIKFITTVQHGIEESKKNEGSMVAFGTFAVMGITYL
                     AISIASGYVGYTMITKFKI"
     gene            complement(26492..26758)
                     /locus_tag="I9760_000587"
     CDS             complement(26492..26758)
                     /locus_tag="I9760_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042377.1"
                     /translation="MSKDEIARMFSSTFKQASNNISAQVFSSSIRFIAISLVILAVMW
                     CVNHMMGTEEKQQQGYLLNFGSRMVRLVIGLMIFILVLFVKETT"
     gene            complement(26758..27084)
                     /locus_tag="I9760_000588"
     CDS             complement(26758..27084)
                     /locus_tag="I9760_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042378.1"
                     /translation="MKRILCLTLMIISLQCSANNPDLNETLVRIINQINAIMPLLDEA
                     QTQITPNARIQLQIEGFEDLQGQHHSGLREDLLNIRNGLIDYINQPIIAPRKIKPLEM
                     DFVRKP"
     gene            complement(27094..27726)
                     /locus_tag="I9760_000589"
     CDS             complement(27094..27726)
                     /locus_tag="I9760_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HAT5042379.1"
                     /translation="MAVKQYSRQMTMQLTIVLFLGWLAIIIWALSQWLLHGYSFAFEK
                     VNILVNTQREAITPVYQGSLLVVLPLNAKPDWVFTIPYFNKLAINDLAKDLIEKSQQF
                     VQILLLSSQCLLIKLIILFAAMPLFALTMIAGLVDGLNQRAIRTACLGRESSYVFHRL
                     NHYLKKGLVILLILWLALPVSITPAYVFVPVSLLMGLMVAMTASRFKKYL"
     gene            complement(27717..28148)
                     /locus_tag="I9760_000590"
     CDS             complement(27717..28148)
                     /locus_tag="I9760_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HAT5042380.1"
                     /translation="MIRILLLLSVALNVHSMNVIPLTLSQSDTSLKLNLSSPIGVPAK
                     SKATAGKVSRKTLDTTLLNMPLFVIGADKASIQWLEQHQEELKFMQATGFITNVNDFE
                     TIVALQEKFKLPLLPVNVDPLLAYLHEQHYPLIIAEGALWQ"
     gene            complement(28148..28969)
                     /locus_tag="I9760_000591"
     CDS             complement(28148..28969)
                     /locus_tag="I9760_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HAT5042381.1"
                     /translation="MNKLSTITLCLLLGQQAIAGLYIPGIATSPMQQADNALAKAGLT
                     EFHDEVIEEKDFLLNDVQKQEAKIWGLNEKEEKRYLQLMQSRSGVYYKDLRMTPIDIL
                     GLNARDDAEREHFASLAARQEAQKVAQNIAWNNAFYKAYNELFKDVPVVGDFDPSPYS
                     PYAHQPIQLKEGETLYLFIKPDDAVTTIVLQLIDAINRTPNTKLNLLFLDMNNNAIQL
                     WANRHQIPIQLVTNQQITLTPGNQQFEGLALAKKQTPLLLIANGKTSQVVDLGRF"
     gene            complement(28962..29549)
                     /locus_tag="I9760_000592"
     CDS             complement(28962..29549)
                     /locus_tag="I9760_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042382.1"
                     /translation="MKKFLDAKWLGLVVLNVLVIVFVCVKHQSSSINNNNLNGLEQKL
                     ITIQSQLNKPNQQPDLSPITDNIKQLGDFIQQMQNKDDHQLGDLFSTEQAAIKNQLDG
                     IKELLKHLDSQKQPIKMLPASELPFRVLSIDSVQEISVASVAYDYKTQALEKGDSLAG
                     WKILDINFAKQTIEFENADKAHVLMHLTQQEVGNE"
     gene            complement(29546..29959)
                     /locus_tag="I9760_000593"
     CDS             complement(29546..29959)
                     /locus_tag="I9760_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042383.1"
                     /translation="MLRTTIIGLLALSSLQIQAANITQVGRYATVNNQPLAAQINPLK
                     TVQQIHFPSSVQTIGEAVEYWLRYSGYHLAPQDKQNESLKQIFQQPLPQVTRNLGPLT
                     IADGLTVLVGKTLFSLKQDDLLREINFSLNARRAQ"
     gene            complement(29968..30168)
                     /locus_tag="I9760_000594"
     CDS             complement(29968..30168)
                     /locus_tag="I9760_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HAT5042384.1"
                     /translation="MLILDRKIGEEIYINKGKIKITVLYEKNGLIGIGVRAPSEIDID
                     RKEVFIRKYIQKLDQENKSNQG"
     gene            complement(30162..30515)
                     /locus_tag="I9760_000595"
     CDS             complement(30162..30515)
                     /locus_tag="I9760_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042385.1"
                     /translation="MFLRFNAIYGALWLSAYNNEKALEAAKLEWADSIKEFDSQVLTF
                     AVEKIKRTQQRPPVIPVFVELCISIQKSIKAREEALRAKPENHKRTDPQIVKSHIKEM
                     MEKLTSPSVKEKKSC"
     gene            complement(30532..31431)
                     /locus_tag="I9760_000596"
     CDS             complement(30532..31431)
                     /locus_tag="I9760_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042386.1"
                     /translation="MGFLFVTTNELSALMGLPYIQQSTYLLGIRPYMDRETFLVGVKR
                     KISYQQLAEALYIEPHQGFQQSGSPSRQQLRRIIYALERAGLIKIESIGMNLIVKCLL
                     ADTDISVQNKPDTNPTQQPDTNPNIKKNYKSSSYDDFSKKSNTVENTKPDTPHNSENL
                     CVYVRAQFQKFWELYPQKQDETRAFQEFYKLKPDETLFRQMMDALQEQIENRLDMELC
                     GEWVPKWKFPANWLAQQCWNDELLPIRKQEQGHANIQTSYRKKSAADILAESCKESSF
                     SINFEDKPQTGNNVLHFNSTRSV"
     gene            31600..31854
                     /locus_tag="I9760_000597"
     CDS             31600..31854
                     /locus_tag="I9760_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5042387.1"
                     /translation="MNMKKIIGSRITQARKANGLTIKILAERTGFGAARIGNWEQGTR
                     SPGPEEALVLSKELVVAASWLLGISNDPHGEWMDHVIFEY"
     gene            complement(31753..31995)
                     /locus_tag="I9760_000598"
     CDS             complement(31753..31995)
                     /locus_tag="I9760_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017376762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAT5042388.1"
                     /translation="MLFAIRLYERYGLIETPQRAPNGYRQYLEDVVDCLQLCQTQIAL
                     KAILIFKYNMIHPLSMWVIRNTKQPRSSNNQLFGQN"
     gene            complement(32048..32899)
                     /locus_tag="I9760_000599"
     CDS             complement(32048..32899)
                     /locus_tag="I9760_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042389.1"
                     /translation="MKKFAITLMSLIFINSYYPTAFSEKPNQRMMNNQSTHSLKSKNT
                     LQLSIQEIMDKKDKKLVLIKLIDTKSNKPITLNDLIEAHTQKIHLLIIDDTLLDYSHV
                     HPVETTIPGIYQFEWQPTLKSASYRAWADLIPTNTKTQEYVVADLPYPKTKKKLENPI
                     HRQPLFESSVDGYHFKLSFDKTPLHVGQAVMGKIDITDAKENPVHTLEPIMGAYAHIV
                     GFNEDFKTVTHVHPMGKEPTNNTERGGPELLFHIKPNKVGFIKMFAQVQIDGKTLFVP
                     FGVTVNS"
BASE COUNT         9690 a         6126 c         6899 g        10630 t
ORIGIN      
        1 taatttcatt aagtaaatga aatcatcata aatacctcaa attcacctta aagaagaaag
       61 tatattcata taattcataa aaaatcttga ctgaataatt tcaataccaa ataatggtct
      121 gatgtgaagc atattttgat cagtttgtta tctttgattt ctttatgatc gcagggattt
      181 cttacaatat ggaggggtat tatggtaagt ttggagcata tacaaaaact tatttcggaa
      241 tgtaggaagt tgggtaaaga tggacttgat aatggtacta atgggttaat ccctgaattg
      301 gaaatcgatg ttgtccctcc cagtgcgttt ttaggggtag gtaataatcc ggcaattttt
      361 gtaaattcta aaacatacaa gttaatgcgt acaacccatg aaaaatgggt tgaaaacaaa
      421 acaatagttt ttaaaagtta tttgctttcc cagccggcga tcaaaattat tggtgctatt
      481 gttcatgaaa ctggtcatgc ttttaatgtg gctgccaaaa ttcccaatac tgaagcaaat
      541 gcctgtattt ttgaaattga agtgttaatg aggttgtttc aacttaaaag ccccttgctg
      601 ctagggtgta cggaactgga tatgcaatct tattttaaat cacggttaac tgattacaac
      661 aaatgtgtga aagactgccg atgtttagct gaaatggttc aattaataac tcatcaattt
      721 aagctggatg aggtatcaat ttcagagaaa gaaaatcaaa tcccgcttct gtctatttca
      781 aacaaatggc caggcttatt tgcaaaaaaa cagattgccc ctgacatgga taaattattg
      841 acttctcctg taactatcac tccagaggtt aaaatcctgt tttatcaatt ggttaaagag
      901 cattttcatt ctcctgaaac agagataaag cttgatatat aacaatattt ttgattgatg
      961 caggtaaatt actgtgactt tatgagtctg gcatgcaaaa tgaattctgc acgccagaaa
     1021 atacttcaaa aagtaatgtt taatttactg tggcggaaat aaatcctgaa atttcactcg
     1081 acttttcaca tgcaaagctt ttttcctaac cttggataaa tcaaagacct tatagatgat
     1141 gacgggtata atgaaactca ccacaccaat aataaatgat aatttataga taggatcttt
     1201 atttagcagt aagaaaaata aggagccaac catcaaaatg acagcaagaa ttccggtata
     1261 gggtgaataa ggtgttacat aacggagatc tttaatggag taaccagctt tatacaaacg
     1321 gtttctaaag ctgatttgag caatgcataa agaaagccaa gccagcgttc cggtaaaacc
     1381 ggatactaac aataaagcga tatagagttt ggtttgacca aaaaagtaac ccacacctaa
     1441 aagaatccaa actccaatca gtgtggcaat aacggcgttc tgaggaacag agttttgatt
     1501 aaatttggct aaaacgtgtg gagccatacc atcacgcgcc aaagcattta aagaacgcac
     1561 agcaccataa aatccggaat tagcgcagga taaggttgca gacagagtaa caaaactcgt
     1621 tactgctcct gcccatttca ggccgtagaa attaagcgca tcagcgaaga ctgagttgga
     1681 taacccagct ttttgccagg ggaaaattaa aaccagacaa aatactggaa tgatgtagag
     1741 aaacagaatt ctgaaagtaa cgttacggat ggcgtgaggg atcattcggg cagggttttc
     1801 agattctcct gctgcaagtc ctatgatttc agatccttga taattaacca gcaaaagaac
     1861 catggctgtc aacaacgaca ttgccccatt aggtaacaga cccccatcgc ctaacaggta
     1921 cttaaatcct attataccgg gtggttctga tccatgaatg agtccaaaga aaattagtat
     1981 tgccaggaaa acaaaggcga gtaatgagat aattttaatt aaggcgagcc agaattcaat
     2041 ttcaccaaac gtatcgactt tagcaaggtt aatataggta ataattaatc cgaagcaaac
     2101 cacccatatg taaccgctca ccccagtaaa caattccatt atgatccctc ccgcaacaca
     2161 ttctgcgggg atataggcga cccaggtaat ccaatatgac cacccgacac cgcaagcgac
     2221 agttggggat ataaaatcag aagtgtaagt aacaaaagag ccggaaattg gaatggcgac
     2281 tgctaattct cccatacata acatggttag gtaaataatt aacccaccaa gtaaataggc
     2341 aataaacaca gaggggccaa caagattaat tacttcaccg gttcctaaaa aatatccaga
     2401 gccgataatt ccacctagtg caatcagttg gacatgacga tcttttaaac ctcgtctgta
     2461 accactgtct ttaatgggtt ttgatagggt tggaatatta gtcttcacgg gctgtttttt
     2521 ctcactatgt tagtaaaaaa taatagagct aatgttatat ttatccatat aaatttaaaa
     2581 ctgctccaga ttaaaaaata tttttcatct tattgataac tctcattttt gctatatgga
     2641 agtagctaat aatgacgccc caaaatgaaa aatttcgcgt ttatagcata aattttgaac
     2701 ttttaaaaga aacaatttca ttaattttta tttttttatc tttcttcatt ttgatggaaa
     2761 aatgagtttc agattgatat ggatttccag gtgtctcggt tttattctca aattcagttt
     2821 ctgttttgac caaaaaaaac aattaatgta ggataaatgc gttatttgag gtgaattaag
     2881 gaaagactat gctgcgatgg atcgtgttaa tttgtatctc tttgtttttg cctttatctg
     2941 cttatgcgga caaatccgat tgtatcaatc atcattgcat tgcggtaatt gatgctggaa
     3001 gttccagttc acgattacat atttatgcct atgatttgga tgaaacaaac acacctattg
     3061 caattagaga gatttggaac cgaaaagccg ttcccggatt gtctacaatt gagcatgaaa
     3121 acaaatcggt ttcagcctac atggagaagc tattttcaga tgctccagct gttttaatgc
     3181 cagtttattt ttactccaca gcaggcatga gattgttgcc taaaactcac cacagaaacg
     3241 tcaatcaatt ggtagctgag tggtttgaca ggcagtatta ctggcgactt gtgactgcaa
     3301 gaacgatttc tggtacagag gagggtattt ttgcctggct cgcaaccaat tatcaactaa
     3361 acctgtttca acaagaaccc caaactttag caggtgtgat ggatattggc ggagcatctg
     3421 ttcagattgt tattcctgtt gtaaatccat cttttatgga tcatgagcat acaaccacaa
     3481 ttcgcctcta cggcaaggaa tttacggttt tctctcatag ttttcttggt cttggtcaga
     3541 atgaaatggg gcatcagttt tttgatttaa aattctgctt tccagaggaa tacgaactgc
     3601 caagcgggcg ccaagcccga ggcgatgcct atgcttgcaa agatgaaatt gccattttag
     3661 tcaatagcgt tcattctgtg agccgaaaga ttaaatcggt tttagccgct aatcctattg
     3721 agaaatggta tattttgggc ggcattacct ttatgctgaa tgacaaactg ttttctctgg
     3781 acaatcaata ttttaccaat cgtgatctta tttcctttgc agatcaaagt atttgccatc
     3841 aaaaatggtc aactcttgaa gagctctatc ctgataacct catggtgttc aactactgca
     3901 tgctgccttc ctacctttat gctttgttag ttcacggcta tggacttaag agttaccaga
     3961 aagttaattt catgccaaat agcaagaata tcgattggac cttaggagtg gtattgcaac
     4021 ataagcaatc ttaagtatac ttttctttat gccgttgtag ctcagttggt agagcaattg
     4081 attcgtaatc aagaggtcgg aggttcgact cctctcagcg gcatagtaaa atcaatgagt
     4141 tacaaataga aagttaaaaa ttgaaaaaat ttggtaccgt ttttggtacc agttttgaaa
     4201 tgtaattaat agacattaat tgatatcaaa ataacattag gtattctggt tctttcccaa
     4261 ttaggagcag ctttaatcat tcgcagaaca ccctaacacg aaccctataa ttttctaaaa
     4321 ccataagttc ggtttaaatg agaacaaaat acaatataaa tgatcatttt gtgagtttat
     4381 atagatcata gtgtaaaaaa acactcctta atatgattgt cttgatcaaa taataaaatt
     4441 ataatccaat actgttattc aaggatatta attaggagat gttatgaacg tatattattc
     4501 taatagaatt gataccgacg aagataagga tgaatatatg ccttggtaaa taaaggagat
     4561 ataagtatct ccttttatag ttttatattg atatgaaatt agtttttaac aatgatgttc
     4621 tagcggtcat tgatgatttc ctggagcaac catcttttga aaaaatttgg aattttattc
     4681 aaactgaaaa attcaagttt gttcactctt ctaaatgggt aaatgctttt agtctcgagg
     4741 atggatctcc tctatggagc agcgttacta tttctcatcc taggccggaa gcttgcacaa
     4801 cagagaaagt ttaccctaca aactcaacca ttgatttatt tataaatgaa ttaattgcaa
     4861 gatcctctga ttattctcat ttaattggtt tcaaggatag agattgggat tttttctatg
     4921 cgagacctta tctatatcct agaggatcag gactttcttg gcatactgat ggtaagtata
     4981 aaatttcagg agcctatgta tattactgcc atccaatttg ggacataaat tggggagctg
     5041 aattactaat aaatcctact cctcaacttg atttcgatta tcctgaagtg acgttaatca
     5101 atgataagaa gaaaaaagta gggtaccatc taaatagcgc tgaattgaat aaatatgctg
     5161 atgatggagt tggatattat attgtcccaa aacccaatag gttggttatt tttaaaaata
     5221 atattctgca ctgcataaaa aaagtagaaa aggcagctgg taatcatgta agagcatcaa
     5281 taacgggttt ttttatgagc aatgagaaaa tacatgaaac gaaagaggcg gaaaaagaac
     5341 attcaatcta acaattattt taattagttt agccccacca aattatagat aactaatctg
     5401 aatatgcttg gaattatgaa atgaacttag tgcatcaaat cacacctaca ctagctcagg
     5461 aaaaagtaaa ttttgttatc tcaatacccc attctgacga ggcagcttta tctaatttta
     5521 aacttagagt agaggatatt ccttataatg aacaaccaag ccattatcaa tttaaaaaat
     5581 ggtcttcttg gtgttcggct catttctctt cctattacta ctacattaag tataaaaatg
     5641 ctctcttaag atgtaagcaa tttttgtatg actttggacc tttatcaaca ttagatcacg
     5701 ctgctcttta cgatcattgg gtttgggaaa tgaaacccca cgtattgaat caggaaaata
     5761 tttgttggat tggttatgac tattcaaaat cgaaaacatt aagtttttta tctcatggaa
     5821 ctaatattga acttcgtctt cttgaaggaa atttgagcga tgaagagttc ttggaacttt
     5881 gtaaattttt ttctcctctt tcatcatcaa gtgaaatctt agaaaagagt ttttttgaac
     5941 tatcctattg gtcacgttac cgcaactgca tagaaaatgc gtgcattaat agaaatgaat
     6001 ataaacctcc ctcaagcata tggaatttaa gatggccaat gcataatatc tttagaggca
     6061 gcaagccgga ttgctatatc aagtactcaa accaacttaa cttagacttt attccagata
     6121 gccaaattat ttatggtaac gaaaatcaaa tagaagaaat tcaatttgtc ctgtttccga
     6181 agaatggaaa gaaaaatcaa atggcatggt ttcgtgtttt caataaagcc tcttttccaa
     6241 taaagaagcc agccctctct agattgccta gtatgaattc atttagtggc tatagtaatt
     6301 ttaatttaac cgtcttaagt ccgaagagta gtatgattgc gagcaatata tacattgcaa
     6361 gtcttaataa ttgttatgga ccacatgatg ccctttggtg ggataatgaa aatgcttatc
     6421 tactgcaact aagcgctagc gctaataact ctatagcgta tttgaaggga attctttgtc
     6481 aacttataga agacaaatga tcaatagatt tatgcacttt tagtatggat atattaagct
     6541 agcgcccaaa gccactagga aggctccaat ccactgatat tgggaaatct tttcaccaat
     6601 aaaataagca aatatttgtg caaataccac ggaggtatta cgaagagtaa gggctagtcc
     6661 agcttcagaa tgacgcaatc cgattaaaaa tagcaagaag gacaatgcac ttataattcc
     6721 tcctagagaa gttattttcc aattggattt aatttgctct ttgaatactt gtttttgttc
     6781 tttgttcatc aaaagataaa cacaaggtaa agcaatccat aaagctgcag aaaagagtgc
     6841 agctgggttt gccccatatg ctaaagaacg accgtagcat aaatgataac cagcaatgca
     6901 gatgccacat aaaaccggaa ataataattg ccttgattga tcggggttaa aagtttggct
     6961 tgtaagtatc aatccaaaaa gaactgatga tacacctatg atattagata aatttatatt
     7021 ttcaccaagg aaacatgccg aaataaacca tacaattatc atggcgcttc ctcgcataat
     7081 aatgtaggcc ttcccaagtg aatactgact aagacaaata gctagtgtga ttatataacc
     7141 tccttcaaat aaaccagagg ttagtgccca taaaaaggaa tcagatgttt taaataatgg
     7201 acctgagaaa aaaggaataa atacaagcga gaatatagtt gcaaaactca taattcccaa
     7261 tacttcgtaa tagggtgttg aattgcgttt ggcaaatgta ttccatgctg catggaggat
     7321 tgcggataaa attataaata caccagaacc catttcagtt catttcgaga taaataatcg
     7381 attattgtaa cattggacca acagcctata acataaaatt tttaaaacct aatagaagtt
     7441 gcaaagtgat tttagagttt aaagtattaa aaaagatgcc ccaaagaggg gcttaatagt
     7501 tcgcaaaaat atatgcctgt ccaccaaact ctaccgaaca ataatcacta ataaaaagat
     7561 cccgagcaaa agcttcaaaa tcaaaatagc agatcaaatt atccggcatt tgatgggcat
     7621 aacagtcatc aaataactgc ctagaaaaat caacctctga atcataacaa ccttggtaat
     7681 gatcctccaa catggtttgt gcatcatcta ccgaataatc gcagagaaga gctaagccta
     7741 gctctccgtg ttctttaata aatgaagcat actccacaat attacttatt gactcatact
     7801 catggatttt gatgctgccg aatccttcat aatcgtgtat ggcatattct tcagcattgg
     7861 gttcagagct gttatccagc atttcccaga tttcttccat aatgtcatct tcgctttgtg
     7921 tggcatctat ccaaacacca tgcaggatgg cactgttgta tgaggctaaa cacgcgacgt
     7981 agattgaagg ggtgtccatg ggattatctc cttgtataaa gggagcaatc ccacacggga
     8041 gcttgctccc gtggtagggt atgattaaaa cggaatttct tctgaatcgg tttgcaacat
     8101 ttcacgcatt tgcaccatgt ggtgctctgc ggtcttgttg ctagattcat cagcagattt
     8161 gaagtgacta agtagctgga tatgattgac gacgacgctt aaggtttgat gtgtcttacc
     8221 ttcattgtca gtccattgat tagaacgcag tttaccttca aggtatactt gcgaaccttt
     8281 ctttaaatat tcagctactt tggtaagttt tccaaaaagc accagttgat gccattccac
     8341 tctggtttgc cattcatcat tcttcttgaa tgattcatta gttgccaaag tagcggtgac
     8401 aaaagattgt ccgtctttcc cagtgatgtt tttgggatca gcaccaagat tgccaattaa
     8461 ttgaacctgg tttaaagatg cggtcattgt attctcctgt tattgttaaa tgtgaacggc
     8521 ataacagaaa aacatccgca taggcagatg attttctgtt atgcggataa gacttaattt
     8581 gcttcaatta acaagcaatc cgttgagtat caaactgttt gacaaatgaa atcagggact
     8641 ccagtttgtg caaacttaac tctgcttgct gaatgtcata gggattaatt tgcagtcggt
     8701 gacgacttaa atcattgaca caacaattca gttcaaatag caaatgttca atttcaagtt
     8761 gcgctggttc ctgctgttgc atggtatgct ccttaggtct aaagaggatt agccccaaag
     8821 ggactatgaa tcccaattgg gttatgcaaa aaagatcacc gaaacttcgg ttgatttacc
     8881 tggttattaa tgatgatgtg ctcgaagcac tcctcgggcg taaagccgta aagacaactc
     8941 ctgatagggc ttccctgcta aacgctttgc catggaatta gggaacaaga tggcatatca
     9001 caagacctgg tggtttcgtc agaattgcac tgactcatca gttgtgttgc tttcagatta
     9061 tcaagccagt attagaacgc aagcgttttt tactgttgat attaaatgaa cgtatttgaa
     9121 tcagtcacct aaatagatag gcatatgaat ttgttctata attgatcaat ataattatta
     9181 cctgttaggt gaattatgtc tgatcataga aaaacaaggt tagcgtttta ttttctttgt
     9241 gagaaagagg cttgctcaga gagcttttcc ttagatgagc tggagcaggc tgctgagtgg
     9301 agtgcatcga ctgtagatac atatctgagt aagaaatgga agcatatagt tagcaggtct
     9361 gcggatggtc tttatacctg tgctggtata tgtaaaatgt ctctaaatga gttcgtaaat
     9421 ttgcaaaaac aaactgctta gcttggcatg aaaacagttt ttttagaggg attgaagttt
     9481 tttatggcca ccatttaagg aaagcagttc cttcttaacc gaggtaactt ttggttatag
     9541 atattctgga gtggccgagg tctctactta taatttcacg agcttctctg tctatggctt
     9601 tttctgattg agataaatat ttagatgttt ttccaccttc aatgggacat ttgaatggtt
     9661 gtttatcagg cgataactgc ctggttaaat cgtggtatct tctttgagca taagcatgac
     9721 gcagaccatg acaatttttc aaccctaact tttctgtctc agtttgatat tgcctcagat
     9781 actgtttata agttttattt ttggggatca atgaatggcc aggagagatt tgttttattg
     9841 ttttttctag ccattctttt tgttccttgc ttgtaatatc tatggctctt ccaattccgc
     9901 ctttagtcca gcttgatttt aatatcaaac gttcgccaaa ccaagcttcg cttataacca
     9961 atttcatcga ctcttcacga cgtaaaccaa agaggtattg agcctcaaga gataagcgta
    10021 tcattggatt ttcgcatttt gagaagtcta tattagtgat agctttattg tatgtgggta
    10081 cataatttcg gttatcaatt tgataatagt cattgctagg tttgattatc tctggtttat
    10141 taagccagat ggataatttc ctgagtttag acatgtaatt tttgatagtg gccggatttt
    10201 taccctgttt tttccagtac tctactaaca catggatgtg ctttgatttt agtcccttga
    10261 tatgagctac cataaaacct tgctcatgca gatccttgat gcaacgattc aatatatggc
    10321 gtgtatcagc tttactggca tgggaatata cacttagttc tttgatacat tcgttgatag
    10381 aaaatagtgc gcttttaagt tttgatttac tcatgcaggt atgtccatag tgtgttgcga
    10441 gattttattc caagttcatc ctgtaatatc aaacaggttt gatagttacc taatattggc
    10501 agtaagtatt gatacatttg ttttgcatat agataacgcc aggacttttt tacgggtacg
    10561 tcaaaagcca tgagggtatg attccagcta agtttgactt gtttataacc aaaaaattgt
    10621 accaaacttt gtctattagt tagtttttct aagtcagata cgacgttttt ttgaatttca
    10681 tttctgaaag ggatcgttct gtccaccgag ttaaaagtga tttctctcgt gagcgaaagt
    10741 ttatgctctt tgatatggat tccaggttta atctgtattg cttcactaaa ggtcaaacca
    10801 aactctgttt gtaaggccat aataatgcgt gatgtcattt cagagaacga ttcccagaga
    10861 ttgggttgcc attttaatcg ttggggtttt agtatttttc gatgcaattg cagagacttg
    10921 ttgtctatat tctgtattgg acaattaatg cttagtaaat aatttctaat aattgtcata
    10981 taacgcataa ttgttgcatc tttaacatgg tttcttttcc aatatccaac cagtttttga
    11041 atatgctctg atttcatgtt ttgccatgaa gtaggaacat ctccaatacg gaatagatca
    11101 tcaatcatct tttgtatgac gtatgcgcgt tttttctttt cttggtagct gcctcggtta
    11161 tcaagttgaa agtaccggtt tgcagcttgt cttaaggaat tttttctcat catccaatcc
    11221 gaaaattcat ttgccctgtc tctggtttag tgttggtcga gcggctcact caaagatgaa
    11281 acctggcaaa agccataaag ctcgaagtgg gggcaaaaca atcgttataa ggcaaacgat
    11341 ctcgccgatt tacaacagtt tttctggtta tttcctcctt gtgttgttga ttaataaaaa
    11401 cgacaaaacg atgggctttg tcacaagtcc atggtcaaat tgaaataaat tgacttaaac
    11461 tcccgaggga gttgccctgt cggatgacag agattcctag atgattttcc cgactgaacg
    11521 tccagccaca gagttaggga ataaagtggc tttgcacaag acgttggatt acgtttcgct
    11581 ggaatcgcgc cagctcttca gttgtgcttc attaaaaaat atcaaagtgg tgcttgctgt
    11641 gcaagggtta aaagagaaca attttgtcct gacgccagtc tggcgtcagg acaaaataca
    11701 gtaatgtgtt gattttacga agtaaaatca agggttgtgt tctgcgtcac tgcacataaa
    11761 ttgcagtgac gcagaaaaaa tgcattgtca cagatgatga atggacatcc aatgtgctat
    11821 atttttatat aattgtcggg gctaatggag tagtgatgaa gaaatcgcaa ttatcaagaa
    11881 accatatcat cacagaccca attcatggtg ttatgtcttt tagcaaagac gagaaggaat
    11941 taattaagca atttattgat aaaccgattt ttcaaagatt aaaaaggatt aagcaactgg
    12001 gttgtgctga tatggttttt cccggagcag ttcatactag gtttagccat tctcttgggg
    12061 cttgttatat aggtaagttg gtttgtgacc aattagacat aaataatggt gatagaaaaa
    12121 ttataatggg ggctgcttta ctgcatgata ttggccatgg gcctttttct cacgcttttg
    12181 agtccatttt taaagggctc ggtgagggaa aaatatcaca tgataaacaa tggactccta
    12241 agtttattga ttcctttgaa tgtgacttaa aggaagatat tcttaatgtt tttattgatc
    12301 ctaatcatca tgagtattat gacccattga ttgcctcaca gttagatgta gatagattag
    12361 attatttgct gcgagacagt catttttgcg gagttccata cggcaatatt gacttgcaat
    12421 ggattatatc ctgtatgaaa caacaagaaa tcaatggtga cacagtaatt tgtattactc
    12481 ctaaaggtat cggagcaatt gagcattata ttttgtcaag aagattgatg acaaaataca
    12541 tatactatca tgggaagaaa aactcatcag aatatttaat ttcaactttt ttaaagaatt
    12601 tagaaggtca tttagatata gcagaattag ctcaatcgcc attatttttg tttatgaaga
    12661 attactttga atacaagcga ataataaata aaaatactga caacaatgaa tctaatagaa
    12721 atgattttgt tgataattct tttaattatt ataaagaaat caccgatgat gatatttgga
    12781 tagttttgag aagattgtca aacgaagatt caattgctgg acgagtggcg cacaagattt
    12841 gtaacagaga gttgccaaac agttttctaa taaatccttc ccgaagattt gatgcaaaaa
    12901 aaataattga acgatataaa gatcaagaag atgaagataa taaatggcgt atatattttg
    12961 atgaattaaa ttttgaatct tatggcagta aaaagatacc tatttatgtt tctgatcaca
    13021 atacggttac agatctatat cacagctcaa atatgcttaa tcagatttca gacaagcctg
    13081 agccaacata ttatgtatat atagatgcaa aacatccaaa agaagaagag ttattgtgcg
    13141 agctcagttc ctatcattgc cttgtttccc cttattttaa atcaggctag aaagtaattt
    13201 tagaacctac tccagttttg catctatttt gtaataatag tttttttatt ttccttgctt
    13261 gttatatttt ggttggaatt tcgatatttg tttggcttca cttttgcgag cttcttgtat
    13321 cgatgaaaat tgctttattt taacctgagc tccaggaata gtgccaatat gttcggcaat
    13381 cctgcctgtt accctgcctc ttttagctgt gcctatataa tttttattgc cacctgctgt
    13441 ttgaatttca tatacgacag gcttgttatt tggtaattgg ttgattaaat tagagctata
    13501 ttttgttgtt ttcatgttac actccgttgc taaatcatga gatctcatat tgacaaaaat
    13561 gatcaaaaaa tgcaacaagt tatgtataaa aaattgatta ataatgaggg taggttcata
    13621 tggcatttgc ttgcaaagag atgggggatt ttgatgttaa atactgtgta ttattgggca
    13681 atggaattct aattttaaac agttcgcctc cattaattag aataaatgcc tgtccttttg
    13741 gcaaagaaat aatatcatct accccaatca ttggtacaga agaagtttga acccgatcct
    13801 cattagtcgt attaaaataa accccatcgt ccccatgagg tgtatcgtta accatagaaa
    13861 cctgcgtatg tccaacaaca ccaacttgag gtagcacttt aaccagtaaa ttggctgttt
    13921 cttcattttt aatacgaagc ataatcaggg tattaaaatt tccttcggat acttctgctt
    13981 tagccctgga gccgagtgca acttccatat cctgaatagt ctgagcataa gcggtgactt
    14041 gaaacccagc accacctgct ttattgagaa tcttcacaaa agagtcctgg ataatctctg
    14101 acaactcatc acaatgcaga tttaaagtat atcgtgcgtt gggttcttta tagattttac
    14161 cagctgtaga caccagatca gacaaaaagg cttttcctac tgcttgggca atattgggat
    14221 tggttagact atctaagcca atataaagca ctttcttatt tttaattacg tccatcagct
    14281 caatatcgcc tggttgagct tttgacgata ggatttttga ggcattactt ttattgattt
    14341 cagacagtac aggtccgaca ctggcagtga ttttgtcgta ataatgttta tccataatag
    14401 ctgcatcgta gaggtctatt aagatttggt cgtgtagggc ttcagcattg ttgcttttga
    14461 tggttttgtt gatgtggtct ttgacatact gaatgaccgc ctggtggcgt tccattgggg
    14521 attttgtttt attatttttg cctattttac tatcatgttc ctcgatgatt tcatctatct
    14581 cttgatggta atttgtgtag tggttaggaa gaattttatc agcataggac attaacagtt
    14641 gttctaatcg gctgatatag aaggctattg tttgataggt aatgggttga ttcatttctt
    14701 ccaggcatat agcaacgata ttaacgtatt tccatgcgaa ggctgcgaac tgctttcctt
    14761 ctccctctgc ggatattgca tcagtgatgc gtgtggcaac ttcgctgatt tggtcgaaat
    14821 tctttaaagg gttgtattgt gctgattgtt ctggaaagcc taaatgtact attttgaaat
    14881 cttctattcg tcctgatact ttacaggctg cgatcatatc tctaaccaga tcttgatcac
    14941 ctttggggtc tacaacaatg acagcatcac cgttgcggat gtcttggtta atcaaaatgc
    15001 tggctaaacg ggtttttccc actcgggttg taccgactac gaaagtgtgg cctactcgta
    15061 catcttgagg gatgtataca ggtttatctt cttcgccaac gccatgcaaa aaaggactac
    15121 cgccaacagg tggatcaggt ctaaaagggt ttaattttga attggtattt agccatttgg
    15181 ctagctttgt atgttcatgg ttttggcaga atcttctggc aagctggtag caatggcttc
    15241 gttgcatgaa cttttcattt ttgacttgtt taatttgatg tagcttttgg gtatggcttg
    15301 gtcgccagcg aaagcctttg cctaggaaca accagttttt ggataaaggt atttctgtgg
    15361 tggatagggc ataggtgggc atggctaata atcttcggtg ataccgtttt actttaattg
    15421 cttgccaggc tctataaatt ccagggatta ataatgcgag cgccgcatat aatcccatag
    15481 tctgtgttag taaaaacaaa tgcggttggg tatatgccag taaggctagg gacaaacagg
    15541 taatggctga ccaggcttct gttggttctc ttaataggtt attaattggg taattgctca
    15601 tagtcggctc ccaatcatca aagcatcaat gaatacaaaa atagccagca gataccaacg
    15661 agcgtttaag gccgtgttca gttgttctgc ggtaagttgg tgttgattct gtaggcgtga
    15721 aattagcttt gaccataata ggacaagggc taaatagagc accccatgca ggacgagaaa
    15781 taatggttga atggtagtga aggtgctttg ccagcgtgcc agtagattgc tattaatgaa
    15841 ccagggcgca attactaata tggcaatcat tggtaaaacg gtttgaatta atactataat
    15901 ggatgaacgg atataatgtt taatcatagg gataactcca aatgagaagg gtatttcggt
    15961 tgttggccgt tgttcttgat gccattgttg gagtggcaga ggataaacct gccaaacgct
    16021 atatgtctgt ttatgaggct caagataaac ttgataatgg cttgatcact attcgtgaat
    16081 acaatgaagc cttcgagcgt aaagatgtgc tttaacatgt tcatcgaatt ccttttgcaa
    16141 taatttttac tcctgtcttg gctgcctgca taccggattg cgccatactt tcgctctgcc
    16201 tatccgaacc attaaccaaa tccataagac cagccccagc gtcaccgcca aaatgacttg
    16261 ataatctcag caataaaaca ggcgcgacaa agtaaaacat cacagccata ttcttcatgg
    16321 cagaaatcgc atcgttttca cctaatggat caagtactga acgttcaaga aatccgacta
    16381 aatgccagag gtattgaaga aaaatagcca tgacaaataa ggcacacagg ctgcctaatg
    16441 ctctggtgct gtagcagctc aaagacaaaa ccattggggt gagaatgatt aaaaagaaca
    16501 tgaaaaacgc ctgcattaca ggtaaggttt gcatgatggc ttctcttttc aatggggtag
    16561 atgtccagga ttttgtccat tgcccgacat tgaccaagct atgggtaatc gcggatgcga
    16621 cctttccatt gttattgtcc atgaggtttt gcacagaact ggtctgcatg tctctgcttt
    16681 cttgcaaaag cattttcgca ataaaatctt ctgcgctgat gtctgctttc caggctttcg
    16741 ggtgtttcac tttatattgg cgaaccctat ccagcatggc gtagtaattt agatgtttgt
    16801 taaaaacact ggcctgatta gagacttcaa ccaggtcagt tttgattttg ttccaccatt
    16861 gctgacagct gggataacca tcttctggca atcgttcggg tgggatatcg ccacgattag
    16921 ctgccttttc aagattgcga ttaggggctt gatgaaaggt aaatcctggc acgggttggc
    16981 gagcatgaag tttgctgtaa tacatttttt gcaggatttt agaacccatc cagtttaagt
    17041 catcttcacc gccatagcgt ttgagcatag ggtctaattc actggcttca tgcttttcac
    17101 tattgaattg agttctggct tcaataaaac attgtctatg aaaatccaat gcctgcttgc
    17161 gaacgctctg ggggagatag gttgatacca aatctccttg aatggattgc aaactatcgg
    17221 tgcaacccgt caccttcata aggctgtagg taaagctcga tataaaattc tggatgatgg
    17281 caaagcctat aggaattttg acctggttag tcagtagatc tgcaaaggct tcatcataag
    17341 ttgtgccgct gtcgccaatg accgcagtcg ttgggttttt cagtccgcat aagggtttaa
    17401 attgtagggc tttggtttcc aacggtacac aaggatagac aaataagctg catatcagga
    17461 aagttattgc caattcataa agaaaattat ttaaagcatg ttcaaccgca tagaacgcgc
    17521 cagctggatt gagcacattt ttaagaaagc gataaccaat ggccaaaaag ccaagataca
    17581 acagccctgt ttgccacagg gcgttgaata gaacctcata ctgttgccag cctagatagg
    17641 tggtatagag agataaagga ttaaagacaa tcatgattaa gctcctttat tttctttaac
    17701 gtagattgcg ccgtttttaa cagcaggctg ttcgtgatcc tggcctggca tattttggct
    17761 ttgggcttga tggcgaatgt ccatgatggt tcccaaagtc tcagtcatca tttttttgcg
    17821 gacatcgttt tcaaaggaca aagaatgaat gtcatcgtct agttttttca gggcaaacaa
    17881 gaccatattc atagcaggtt ttaaattctg tacttcttgc acttgtaatc ctgcttgtaa
    17941 gatgcggcgc atcattaagg ctttatccag catattttga acagcgattt cttctgccag
    18001 ttttgagaca gtaagcattt gttcttcacg gggcaggtgc tgaatagtag taatgacttc
    18061 atcagtaatc agtaaattgg aggcactgac tttactcaga ttttcttcag taagcgtcca
    18121 agtaccattg actaaattcc ataaggcctt ggcaatgttc tggctacagg tattggcgtt
    18181 cgcgcagctt tgcaataaag ctgataaacc aatccctgct ttagcatcat ggctttgttt
    18241 gttttcactg ctgctgacct ggatatcgcc aagaacctta actgcccaat tagcagcatc
    18301 ggtaggtgtt ggccagcttt gtaccatagg aattttggtg cttggttttt ccaaagaatc
    18361 taaaggtttt ctctctaaaa gaatgttgta gcccgcaatg actacatcat taattacctt
    18421 aatggggcgt tgaaacttac cgcccgcatt gcctttattg ctgcctatcc agggtagacc
    18481 atattcatct cgcttttgag cgatgctttt tgatgtttcg gtcacatcga cgttttcctt
    18541 tttagctcgt ttggcagcat caagccatcc ttgactatcg gatactgata atatgccttc
    18601 cattggagat tggttttgtt ccagtgcttg tttgacttcc tgacaatctt taaccttgag
    18661 ggtaaattcg ttttgtgcac tggatgcagc gttttgtaga acattgtata aagcaggcat
    18721 ggactgttgc agcttataaa gagggaatcc tgcgactgaa ccttttaaat tatcaatgac
    18781 accaccaggg atatttaatg ctgatttttt taaatcctga aaggtattgg taatggatac
    18841 gacaggatta aaaccgctac aagagaatcc catacggcca tcaatatctg caccaataac
    18901 cagcgtttga tctttattaa caggtggtat aaataaattg gaggttcctc ctaattcata
    18961 atagtaatct gattggctgg gcataaagtt tccggcatgg gttaggtgcg gaataagtaa
    19021 ggttaataat agatatttat tcatgttgat gtcctatcgt ttttgaggtt gtccaacttt
    19081 tgggaaagtg agaaagttca cgagtttgcc tttgttctga atacagccgc gatatttgcg
    19141 ccaaaggaca aacacgtaat tgccatttcc ggctttttcg tcttcaatgc cgaaatcctg
    19201 tgcatcgcct gggtggatat tgcgatttaa aggatagatt tcctgccaga tgactttatt
    19261 gttttgtggg tcgtaaatgg cattagccac cacacaattt ttaccgcagg aattattggt
    19321 tgacttggtg acatgcaaag gatttttatt ggtgacaata tctacggcgt gcatggctgt
    19381 aacaactgaa gctctgtagt tataaggttg tgttacgcgc atcaacctgg gaatttcaga
    19441 accccaatgc tgtgttaatg tgccaatttc atgattaatt aacagatgag gatgcgttcc
    19501 catatataac aattcagctg cttctgttct atccatgact gcatcagctt cggccagata
    19561 ataaggaacg ccaaaaccag tttccggcct gtgtgataga taagggatac ggtaaaatgc
    19621 agcagggctg ccaatcacat caacaatgcg tgtccgctca tcattgatgt gaagatcagt
    19681 ggtttgcccg ctgtcattgc caaatcctaa cgctgaacca gttgccgctt tataggcttg
    19741 ttggtacatc gttcttgctg ctttattttc ataaagcgtt cgagcttcaa gccagggatt
    19801 ttcttctggt ttattgctga cggtaatcac taaatcaggt aaaaactgtt caaccgcagg
    19861 agtgacttca agtcttggcg gtaaacgtcc caccgtccaa gtgcaagaac caatgacttt
    19921 gtaatgactg ttttgaaaca gtttttgcaa aacccttgta gcgattgtga aggtattcac
    19981 aggatgaggt gggcttgtgc tttctaaagc aagggcagtg ttagtcataa aaataagccc
    20041 tgttatgagt agttgccttt ggttgttcga gtttcttagc catgatttca gcggcgacca
    20101 ggacatcatg ttctttctcc aggaggtggc gttgcgcttt ttctgatttt tcagtcatta
    20161 agagggcaag caagtagcga ggtggaatga cgcgaaataa tccttggtag cgtgacccta
    20221 ataaaactgc ttctgcatac aaaccttttt gcgaatcaat atctctgatt aatgtttcct
    20281 gctgcggtgt taaggattta aagcgtttca catcagtgat ttctttttca tccaatccca
    20341 ataaaatcca ggtttcaatt agcgataaaa tctttgcaga tttttcagaa ttcatgtcgg
    20401 taacgttttg ggtaattgca accagccaaa gcccgagctt tctggctact ttggcaacca
    20461 gtagacacac tgtcaccaca gaaggaattt gaaattgcag gtgtgactca tcaataaaaa
    20521 gaaaggttgg cctattatcg ttttgagtgg cctcagccat ggccagtatt cttggcaaca
    20581 aagagaccat gaccaaagcc agttttccgg tatcatcttt aatggcggaa atatcaatat
    20641 gaaagatatc aaaatcacct aaaggttctg ttggtacatt aaaaaagcgt gattttgcgc
    20701 tgttaatgac ataactgttt aatcggtcgt gcatatccag aatccggtct ttcttgcgcg
    20761 ccaccgtttc tttatccaat cgccgttgaa acgaactcac aatatgctca gtcagcattt
    20821 gtggtatgcc atcgttaaat gaagttagaa tggcatcaat taatacttca attagcagcg
    20881 tttcatcagc cagggtaaat tgttcttctt ccatggcatt ggcttcagta atcatggtac
    20941 gcaaagctaa agcaagctct gcaaggtatg cgcgtgaacc atcaccacaa gctaaatctt
    21001 ctgaatgttt cggatcttta agatccatga ttttttgagc tattaaagca gcttgttctg
    21061 tgcttaaatt atctgttatt tctggaattg ctttatacgc ttcacaaaat ggatttaatg
    21121 gcacagcttc ctctttttta ttgcttaata gtaactgctt ggttttctta ccatgagctt
    21181 tggcatgaac gagcatccga tcaaacgagt ttcccatttc aaaaagtact acacgggcgt
    21241 ttttcattgc gagtagtgat tgaatcatcc aacctgttaa aactgactta ccaccaccag
    21301 agttggcgaa aatcgcgcaa tgcgaatttt gactaataaa atcatggtgt agtaagtcaa
    21361 agaaaactgg ttctcctaag cggttgaaac agggaaagca gggtaaatgc cttgcacctt
    21421 ggtttcggcc atagactgga agcaaggcag caagttctgt agcaaacatc agacgatcaa
    21481 aacataagta cttacgggca tagtgaggat cgaaattaaa cggcaacgca ttgagccagg
    21541 aatttaaagg gtgaatatca tagctggaat gaattaatgg catcttggcg tcgttgaaaa
    21601 tggtatgcag gtttttttct atgacttgtg cttgttgctc atcctctgct tgataaaaaa
    21661 cagcctgatt aacccagaac agacgattgc cagtacttaa ttcattacgg gctgttttta
    21721 tatcatctct gacctcttgg ggttttaggc tggttcccac aattcccttt tctaatcgtg
    21781 ttaaatggga atcaagattt tcatcatggc taaagacgac ttgaatggtg taaatgcttc
    21841 cttctggcaa agtatctaaa agcgcatatc ggtgcttagg attggcttgt tgtctttctc
    21901 gggagactaa accaataatg ggtgcttctt ttaagccatc aacatacagg attctttgtt
    21961 ttcggccttc aaaaataaat ccttgctcat cgctctcagg cggggaaaag aatatatttt
    22021 gcgtcagatt aaaacctgcg ggcatgggat tagggtaggg atattgttcc aagatctcat
    22081 cggctgattt cggattaaac cagcgtatcc accactgata ataatcctga cctttaaggc
    22141 gtttgatttc taaacctggt gagcgtagtt tggtttcaat ctggctgatg acttcttgat
    22201 gttcgagaag aatttgttct ctggttaaag tggtttgatg tagttgccta taaaacaata
    22261 ctctgattcg tcgtcttcgt cctcgatagg gcgctcctgt tttgggatcg agaaataagc
    22321 cttctggcct ggtcattttg ttgtagagat cttgcaagcg ctgtaagtaa tcttgcgtta
    22381 gtggattttc aacgagttgt tgtgggattt tggatttaat atgatcgata acgggatcaa
    22441 ggcaatattc atcttgaaca aacatttgca tgacccaagg gtctattgca tggagtggaa
    22501 ccactgttgc aaaggtatcg cggattttat taaagacagc ttgcaagtat tcaggggaag
    22561 cagcttcggc ttgaatatcg cctagctcaa accctgatcc caaacttttt ccatcggcaa
    22621 ataaaaacac ttgttcttgt tcgttgaaat ctacaatagc cagtctgtca gcgaaagagg
    22681 gcaacggatt ggcgtattgc tttttaatcg tctgttcttt gatagcttgg gaattcattt
    22741 ccccaagcaa ccagttagat aatcgtttca tcacgcctcc ttaatacagc tctgatgcca
    22801 gggcatattg attttgctta taaagaaaaa agcgggtggt ataggctggt ttgatgattt
    22861 gttcatcgcc gagctgagcc acatgcgcaa aaatatggat aggtacttcg ggattaggca
    22921 gttgcttaaa ctcttcgccc gaatcctcaa accgtacctg ttgaacaaca gctttttgta
    22981 taggctgctg catgctttgc tgatagagct ggctgacagt taagccctgc tcaggaatgg
    23041 cgctttgcga tatattggcg gttgagcaag cgctcaaagc cagactagcg caaagcattg
    23101 tcaaagttct tttgttgatg gtaaccataa tctaataccc tcccttggtg ttctttatca
    23161 atggctatgg tttgactgaa atgaagactg aattgatttg gtataaagcc tgagtggctg
    23221 cggatgctgg ctggaacaaa aaccatatcg aagctgcctt gtattctttt ctcaagccaa
    23281 tcagtaattt tttggctgcc ctcgttgata gctccaccag ctgcaaaatg ggctaaatca
    23341 ccagtaggcg tggcaatggc tgaaccacct tctgcttgat agctcatttg ccatttggat
    23401 aaagcattgc ctgccccttg aataccgcca gcagccataa aagcagctaa aactctggga
    23461 gcattagtga tgtactgtcc cttaatacaa ggattgccga attgagtagt cagataacca
    23521 atgctgtcat tattcacaag ctctgcggag cttttcattt gttcctgacc aatactgaca
    23581 aaatgcccat cctcaaagac aaataaagcg gcagtgatat acgcgcgaac gcagctgatg
    23641 ttatcgagaa aggagcctac gccaatggaa taaccactga ttttcatacc gataatttct
    23701 tccggcaatt gcatcccatt ggctgtcatt aaatcgccac ggctgaccat tgcggtaaaa
    23761 ggaaataagg gctgcattag tttgccctca acaggtactt cacccattag ggcagacaaa
    23821 agcgtaacct tgctaaaatc actacctgcc ggaatggtat agaagggtgt tttggttggt
    23881 gcttcttctt tggtggatga gtcttggtct gcaaagattt tgctctcaat aggggcttta
    23941 tccagcaatc catccagatc tttgatttgg ccagatacag aagcttgagt gccttccatg
    24001 ggataagatt gagtttgatg atcggtaagt gctgagagct tttctttcag cgtttgcaat
    24061 tcatcttgta gcgtatggct tgcttcttgt gtcggttgct ttaattgttt ttgcagagat
    24121 ttattctcct tgtgcatttg ttccagcttc ttttctgttt caaggagtct tgcagacaca
    24181 tcacggatgt tgtcgttgaa ttggctggta atggcttcat gaaaattgtt tggattaggt
    24241 gttgtgtcat catggatagg cgtactatga cggctattaa taagaatcat aacgactgca
    24301 aaaaccaccg atccagctaa gattttaatg cctttgtttt tgttcatcgg ctgtacctcg
    24361 catgttgagt aagtgttgtg gcaaagggtt gatctgaaac caaaaagacg gtggtgcttt
    24421 catgttgatt tcgtggaggc aattcccctt taggatagaa acttgcggtt tgccaatgtc
    24481 ccatgagttt tgaaaattgc agtttcacgg gttgattcag caggtttttt aaatcgaccg
    24541 cagtgatata gaggctaccg ccacgccatg aagccagagg gtgcgcctca atgcttgcac
    24601 cataaaataa ggggatggtt ttattggttt gcatgggcga gcgataaata ccttctggta
    24661 tatctctgac acgttcaggc gaatacaagg cttgaatggc aaagcgagtc agctggatgg
    24721 cgttgtattc gtattgactt gcacctgact gatgagtggc ttggggatca tcaatcacga
    24781 tttcaatagg cgtggtattg atggctttct cattggcttt aaggtttagt atgatgactt
    24841 cgccttcggg taagagctgc acaatcaaac gtgcatcgtt gaaagcgtct ttggccttta
    24901 aatacaaact gcctttaact ttcagaatat cgaggttggt actgagttgt tgatcaatga
    24961 ttttaatggc ctggggaaat tgaaccagcc tttctttatt aataggcaat tcaattgtta
    25021 gaggaacttt ttcccaaacc agatgttcgc tttgcagcgc gtagcttact tgcgtgagca
    25081 taaagccaat tattaataat atgtgtttaa tgagtttcat gggatacctc cgtgtcgacc
    25141 aataaatctt gtaaacgttc ttcaggacgg gtgtagccat cgagagcgag ttggaaaggg
    25201 ttgtgtaaac gagacaaatt gagtttaacc acacgtaggt gataggctac taccttgtcg
    25261 gcaatcacca taccgctgtt gtctttcagt ctttgagtaa tgcgcagaat taattttact
    25321 tcccaggtat caggaccaat ttttctaata tcttgaggct ccatgaaacg atagagactg
    25381 gctacttgac tgcggtcaaa gagctgcgcg tttttataag ctcccagcgt atctctaagt
    25441 aattgttcat gacgaggggt aaaataataa tgaaaggctt tgatattttg gttaaattct
    25501 tctttgcctt tattagacca ggtgtatagc gttggtatta atgaggacac aaatccttgc
    25561 acatattcat tagggatatc gcttgctttc attaaaccgc cattggctgc catattaggg
    25621 cttagccaaa actcatagcg tttgggcatg gtcatgaggt taatgattaa ggccaaaacg
    25681 atggctgata acaggccgat aaaagccagt aacaagcggt tatggtgctg taggctgtcg
    25741 atttttttcc aatattcaaa catgaggctc tccaacagat ttattctttt gccactgtcc
    25801 ttgataatga atccagggcg agtgtttgag ccttaatcgc accatccata gaatggtttt
    25861 cttcatcaca taaccataag gtttgcctgc tttgatacgg gcgactcctt ttggccacac
    25921 ggtaatggag aggatgaatc caatcacaaa accgatgcag ccagcggcca gtggataccc
    25981 tgccattgtt cctaaaagga taaaaaacaa ggtggttact ggtgttgtag tgatgacaat
    26041 ccaaaataat tcacgcagac ttaagccttt ccaggctggg aagtcatgtg atagatggcg
    26101 cgaagaaggc tgactcataa tttcacctcc tatattttga acttggtaat catggtataa
    26161 cccacataac cagaagcgat gctaatggct aaataggtga ttcccattac ggcaaaagta
    26221 ccgaaggcca ccatggaacc ttcgttcttt ttggattctt caataccgtg ttgcaccgtg
    26281 gtaatgaact tgataaaggt cacgactgcg gtaatgaaca gcaataagcc aagaccctgt
    26341 ttgacgtaat tgcctgttac cgacatcatg cttttggaac cgtcaccaat gttgtccgca
    26401 tcagaaattt tagggaacca gttatctgcc aataacacag gggcgtaaaa aagattgaca
    26461 gctcccaggt agagcgtgcg aagcgttgtt ttcatgttgt ctccttaacg aataacacta
    26521 aaatgaaaat catcaggcca atgacaagcc tgaccattcg agagccgaaa ttcagcaagt
    26581 atccttgttg ctgtttttcc tcggttccca tcatgtggtt gacgcaccac atcaccgcta
    26641 aaatcaccag gcttatggca ataaaacgga tactgctgga aaagacttgg gctgagatgt
    26701 tgttgctggc ttgtttgaat gttgaagaaa acattctcgc tatttcgtct ttactcatta
    26761 gggtttcctc acaaaatcca tttctaatgg tttaattttt cgaggggcaa tgatgggctg
    26821 gttgatatag tcaatcaggc cgtttcgaat gtttagcaag tcttctctca atccggagtg
    26881 atgctggcct tggagatctt caaatccttc gatttgcaat tggatacggg catttggtgt
    26941 tatctgtgtt tgcgcttcat caagcaaagg catgatggcg ttaatctgat taatgatacg
    27001 taccagcgtt tcattcaaat cagggttatt cgcagagcat tgcaggctta ttatcattaa
    27061 ggtgaggcat agtattcgtt tcatggtttc tccctataag tatttcttaa agcggctggc
    27121 cgtcatggct accattaatc ccatcaacaa actgacaggc acaaagacat aagcaggggt
    27181 gatgctaacg ggcagggcaa gccacagtat gagtaaaatc accagtcctt ttttcaaata
    27241 gtgattaagc ctgtggaaca cgtaagagga ttcacgacct aaacaagcag tgcgtatcgc
    27301 tctttgattt agaccgtcaa ccagacctgc aatcatggtc aaagcaaata agggcatggc
    27361 agcgaatagg ataatgagtt ttattaaaag gcactggctg cttaataaaa ggatctgcac
    27421 gaattgctgt gatttttcga ttaaatcctt ggcaagatca ttgatggcca atttgttgaa
    27481 ataaggaatg gtaaataccc aatctggttt ggcatttaaa ggaagtacta ccagcaatga
    27541 gccttgataa acaggggtga tggcctctct ttgtgtgttt acaaggatat tcactttttc
    27601 aaaagcaaag gagtagccat gcaacagcca ttgacttaac gcccagataa taatcgccaa
    27661 ccaacctaaa aatagtacta tggttaactg catggtcatt tgtcttgaat attgttttac
    27721 tgccataaag ccccctcagc aatgattaag gggtaatgtt gttcgtgaag gtatgcaagc
    27781 aagggatcaa cattaacggg cagtaacggt agtttgaatt tttcttgcaa agcgacaatg
    27841 gtttcaaagt cattgacgtt ggtaatgaat cctgtggctt gcatgaattt taattcttct
    27901 tgatgctgtt ccaaccattg aatcgatgct ttatctgcac ctatgacaaa caaaggcatg
    27961 tttaataaag tggtatccaa tgttttgcgg ctgactttgc ctgcggtagc tttgcttttg
    28021 gctggtacgc ctattgggct ggacaaattt aattttaatg acgtatcact ttgtgacagg
    28081 gttaaaggaa tgacgttcat gctatgaaca ttcaacgcga ccgacagtaa aagtagaatc
    28141 ctaatcatta aaatcttcct aaatcgacaa cttgcgaggt tttaccattg gctattaaca
    28201 gtaatggggt ttgtttcttg gcaagtgcta agccttcaaa ttgttggttg ccaggggtta
    28261 gcgtgatttg ctgattggtc actaattgaa tggggatttg atgtcgattc gcccacagtt
    28321 gtatggcgtt attgttcata tccagaaaca gcaggttaag ttttgtattg ggagtgcggt
    28381 taatcgcgtc aatcagttgc agtacaatgg tggtaaccgc atcgtcaggt tttataaata
    28441 aatagagtgt ttcaccctct tttaattgaa taggttgatg agcatagggt gagtaagggg
    28501 agggatcaaa atcgccaacc acgggtacgt ctttaaatag ttcgttatag gccttataaa
    28561 aagcattgtt ccaagcgata ttttgcgcca ctttttgtgc ttcctggcga gcagcaaggc
    28621 ttgcaaaatg ttcacgttcc gcatcatctc tggcgttaag tccaagaata tcaatcggtg
    28681 tcatgcgcaa gtctttataa tacacaccgc ttctgctttg cattaattgc agatagcgtt
    28741 tttcttcttt ttcattcaat ccccagattt tagcttcttg cttttgaaca tcattcagta
    28801 ggaaatcctt ttcttcgatc acttcatcat gaaactcagt aagacctgct tttgctaggg
    28861 cattgtcggc ttgttgcatg ggtgaggtgg caatgccagg gatatacaaa cctgcgatgg
    28921 cttgctggcc taataacagg catagagtga tggtagacaa tttattcatt gcccacctct
    28981 tgttgagtca aatgcatcag aacatgggct ttatctgcgt tctcaaattc gatagtttgc
    29041 ttggcaaaat tgatatctag gattttccat ccggctaggg aatccccttt ctctagggct
    29101 tgggttttat aatcgtaagc aaccgatgcg actgaaattt cctgtacgct gtctatgctt
    29161 aaaactctaa aaggaagttc gcttgctggc agcattttaa taggctgctt ttggctatct
    29221 aaatgcttca gcaattcttt aatgccatcg agctggtttt taatagcagc ttgttcggtt
    29281 gaaaaaagat cacctaattg atggtcgtct ttgttttgca tttgttggat gaaatcccca
    29341 agctgtttga tattgtcagt aataggggaa agatcgggtt gttggttagg tttatttaat
    29401 tgcgattgaa tagttataag tttttgttcc agaccattca gattgttatt attgatggat
    29461 gaagattggt gttttacaca cacaaacaca atcaccagaa catttaaaac aaccaggcca
    29521 agccatttgg catccagaaa tttcttcatt gtgctctcct tgcattaaga ctgaaattga
    29581 tttctcttaa gaggtcatcc tgcttgaggc taaacagggt tttcccaacc aataccgtga
    29641 gcccgtcggc tatagttaaa ggaccaaggt ttctggtgac ttggggtagg ggttgttgga
    29701 agatttgttt aaggctttcg ttttgcttat cctggggagc aagatggtaa cctgaataac
    29761 gaagccaata ctcaaccgct tcacctatcg tttgaactga gcttgggaaa tgaatctgct
    29821 ggactgtttt aagcggattt atctgtgctg ccaggggttg attattcacg gtagcataac
    29881 gtccaacctg ggtgatatta gcagcttgta tttgcaggct gcttagggct aaaaggccaa
    29941 taatggttgt tctcaacata actatcccta tccttgattt gacttgtttt cttggtctaa
    30001 tttttgaatg tatttgcgga taaagacttc tttgcgatcg atatcgattt cgctgggtgc
    30061 tctgacacca attcctatca ggccattttt ttcataaaga acagtgattt tgattttgcc
    30121 tttattgata tagatttctt caccaatttt gcggtctagg atcagcatga ttttttctcc
    30181 ttaactgagg gactggttag cttttccatc atctctttga tgtgggattt aacgatttgt
    30241 gggtcggttc ttttatggtt ttcgggttta gcccgcagag cttcttctct tgccttaata
    30301 cttttttgaa tgctgatgca aagttctaca aagacaggaa taacgggagg gcgttgttgc
    30361 gttcttttta ttttttccac cgcaaaggtt aagacctggc tatcaaattc tttgatgctg
    30421 tcagcccatt ccagcttggc agcttcaagg gctttttcat tgttataggc agaaagccat
    30481 aaggcaccat aaatggcatt aaatcttaag aacaaagtat caacccgttt gtcatacgct
    30541 tcttgttgaa ttgaagtgga ggacgttgtt tcctgtttgg ggtttatctt caaagttgat
    30601 gctgaagctg gattctttgc aggattcagc gagaatatct gcggctgatt tttttctata
    30661 acttgtttga atgtttgcat ggccttgctc ctgttttctt atgggtaaaa gttcatcatt
    30721 ccagcattgt tgggctagcc aattggctgg aaacttccat ttaggaaccc actcgccaca
    30781 gagctccatg tctaaacggt tttcaatttg ttcttgcaga gcatccatca tctgtctaaa
    30841 cagcgtttca tcgggcttga gtttataaaa ctcctggaat gccctggtct catcttgctt
    30901 ctgtggataa agttcccaaa atttttgaaa ttgggcgcgc acatacacac acaaattttc
    30961 tgagttatga ggtgtgtcgg gttttgtgtt ttcaactgtg ttggattttt tagaaaaatc
    31021 atcgtaactt gatgatttgt aattcttttt gatgttcggg tttgtgtcgg gttgctgtgt
    31081 cgggtttgtg tcgggtttat tttggacaga aatatccgta tcagctaaaa ggcattttac
    31141 aatcaggttc atgcctatgg actcaatctt gattaaacca gctctctcca gcgcatagat
    31201 gattcttcgc aattgttgac gactaggact accggattgt tgaaagcctt gatgaggctc
    31261 aatatataaa gcctcagcga gttgctgata actgattttt cgtttaaccc caaccagaaa
    31321 ggtttcccta tccatataag gtcgaatccc cagcaagtag gtcgattgct ggatataagg
    31381 caatcccatc agtgcggata attcgttagt agtaacaaac aaaaatccca ttttcttatt
    31441 catccatcta aaaacactgt tccaaattca gtgtttgcaa tatcaaataa tttcatttag
    31501 tatcaatcaa taacacgaaa cgtgttgttg ataacccatt gtaaataagc actttttagt
    31561 gtcaatgggc tatttccgtt tgatatcaaa tgaacctaaa tgaacatgaa aaaaattata
    31621 ggtagtagga ttacgcaggc tagaaaagca aatgggttga cgattaagat attggcagag
    31681 agaacaggat ttggcgctgc caggattggg aattgggagc agggtacgag aagcccagga
    31741 cctgaagagg ctttagttct gtccaaagag ttggttgttg ctgcttcttg gttgcttggt
    31801 atttctaatg acccacatgg agagtggatg gatcatgtta tatttgaata ttaagatcgc
    31861 ttttaatgca atctgagttt ggcaaagttg aaggcaatca acgacgtctt ctagatattg
    31921 gcgatagcca ttcggggctc gttgtggtgt ctcaatcaaa ccgtagcgtt catacagacg
    31981 aatggcaaac aggataaatc attatgctag gcctaacccc tgtgaattca caatccatca
    32041 aagatcttta tgaatttact gtgactccaa acggaacaaa cagtgttttc ccatcgattt
    32101 gcacttgggc gaacatttta ataaatccta ctttgttagg ttttatatga aatagcaatt
    32161 caggtcctcc tcgttccgta ttatttgtag gttccttgcc cattggatga acatgagtta
    32221 ctgttttaaa gtcctcatta aaaccaacga tgtgggcata agcacccata atgggctcta
    32281 aggtatgaac tgggttttcc tttgcatcgg tgatgtcaat tttgcccatg actgcttgac
    32341 cgacgtgcaa tggtgtttta tcaaaagaca atttaaaatg gtagccatca acactacttt
    32401 caaataatgg ttgtcgatga ataggatttt ccaatttctt ttttgtcttt gggtaaggta
    32461 aatcagcaac tacatattct tgggtttttg tattagtcgg tataagatca gcccatgctc
    32521 gataggaggc actcttaagc gtaggctgcc attcaaattg atagatgcct ggtatcgttg
    32581 tttcgactgg atggacatga ctgtagtcaa gaagtgtatc atcgataatc aacaaatgaa
    32641 tcttttgtgt atgtgcttca ataagatcgt ttaaggtgat tggtttattg cttttggtgt
    32701 caattaattt gattaatacc agtttcttgt cctttttatc cattatctct tgaatagata
    32761 attgcaaggt attttttgat ttcaaagaat gtgtcgattg attgttcatc atgcgttgat
    32821 taggtttttc ggagaaagca gtaggataat atgagttaat gaatatcaag ctcattaggg
    32881 tgatggcaaa tttcttcaca atattttcgt ctccaagaaa accaataggt tttaaaatag
    32941 tttcttatag ttatattttg tatgctattt attttaatca gtaaatagaa tgatatcatg
    33001 attgtctttt taagacaggc attaaatata agtagaggta ccctaaatgt tggttaggat
    33061 cgtcagcgat ggttctttgt tgggatgacg aaatggatgt gatacgtgtc aattaattag
    33121 tttaatattt caaaaaacta actcaaattt tgaatcggtt taggatacga aaaaagtgag
    33181 ctaaaaacaa ttcaactatc attcaattaa ttcaaaacaa agggacaaag ttttcttttg
    33241 cacgtaaatt tttttaggtt tttttgagga cacatcaaga cattttaatt gagcttctca
    33301 aaggttattc ctagctaatt gttcgtcaaa gcatagaaca ctttg
//
LOCUS       DACTJE010000017        31961 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.017, whole genome shotgun sequence.
ACCESSION   DACTJE010000017 DACTJE010000000
VERSION     DACTJE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 31961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31961
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.017"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..107
                     /locus_tag="I9760_000600"
     CDS             <1..107
                     /locus_tag="I9760_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5042390.1"
                     /translation="SKWGCYNKEDIYTKSFEKGLKNLTIIWDLLAMRS"
     gene            complement(132..1520)
                     /gene="legK3"
                     /locus_tag="I9760_000601"
     CDS             complement(132..1520)
                     /gene="legK3"
                     /locus_tag="I9760_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegK3"
                     /protein_id="HAT5042391.1"
                     /translation="MFDRNIKEIINELQEKDLLPDTGKIIGGPRNQFDKKGEIKQGYL
                     FSVMNKEPIGKGGFASVYPVTLYRQGETGFAKKSTDHKKFVTKKFNLNKLKIKYTNLN
                     IENLIEKEFEFCRKAGHLSIKPPVYNNNKCYLTMKRMPGMDLNRIFNKLMANPHYLTE
                     GQRIKITYALLLALKNQVIDKGIIHRDIKPENIHVKVVDPVEVNIFDYGLAKNAGEDD
                     NLFPGSESWAAPEMFIGLKHDSKADIFSMGRVIAFLWGESATNYLSNNPLNKSRNAAK
                     NLNLPAQVKWVEPFLKQMLAERQEDRIEIDSAINQFKQLASINAPKLKLPQSSIKNHS
                     NINNLCFFQNPTKSTNNSDLTQLNIQRDIKKDLQELLENLPGEFSHYRSNIIFKNTIG
                     RFTTIQKMMNELNRIMDENESQDIPKELYDFIHQYQNKFLHASEANIIRKINGFLEDI
                     EYQNLTNSGFEL"
     gene            2120..2962
                     /locus_tag="I9760_000602"
     CDS             2120..2962
                     /locus_tag="I9760_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAT5042392.1"
                     /translation="MAKELIFIDWDDTLVNRENAEDFESVTDENNTIYQMSPMFIGAV
                     EALKQLQEKYHVVIVSSRGDLNRLRQLQLQSYDLESSIMPLENFTFEDNDNRLLFTRY
                     GETNFPDKASFILYIQKMSGNKIKVFIDDDHTEIEAAQSKGIPTIHATHNKYLQNTEQ
                     FFSGLIHQVDNMELSHIFNKVAAEIRNNSRSISWGRQIAGFFSVHLPIAQSLENLASK
                     LQRKSANEVSEEFDQYLKKIAYDPNKKGDIAVSLTKAKTAIDKHISAPIEIKEDQPFL
                     NRMI"
     gene            complement(3439..3873)
                     /locus_tag="I9760_000603"
     CDS             complement(3439..3873)
                     /locus_tag="I9760_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HAT5042393.1"
                     /translation="MNYYSILLTIKRLIIGLLPILLVACTHQQSHGPDHYVVRGKTYH
                     VMKSAKHYKAKGIASWYGSHARHQKTSTGERYNMYAMTAAHPTLPIATRVRVKNLNNG
                     RSVVVRINDRGPFLSNRLIDLSFAAAKKLGIKGIAPVEIEVV"
     gene            complement(4184..5551)
                     /locus_tag="I9760_000604"
     CDS             complement(4184..5551)
                     /locus_tag="I9760_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042394.1"
                     /translation="MALLSFLKPAPYLDEIQDKNVVKRQYRYWRIRTFYAMYIGYALF
                     YFTRKSFTFVMPALQSTLGMTKTELGMIASILSLSYGISKFLSGILGDKSNPRYLMAI
                     GLILTGVFNILFGLSSTWWLFAIFWGLNGWFQGWGWPGCTKLLTHWYSQSERGRWWSL
                     WNTSHNVGGALIPLIVAVCAQYFGWRSAMFVPGIICILGGVFLINRLRDTPQSLGLPA
                     IEQYHEDFSGSTNHHQEKTELSVKEILWNYVLCNQYIWILSISYLFIYIVRTAINDWS
                     MLYLTEVKEYSLITAGSCVIWFEVGGFFGNLVAGWSSDKIFQGKRNPINVLFTAGVFA
                     TLLLFTLTRAYTPFLDALFLFFFGFFIFGPQMMIGMACAELAHKKAAATATGFAGFFA
                     YCLGAVLAGAPLGAIIKNYGWDNFFLTLFVCCLIPLLLMLPLWHVKVNPKYRQSEFSG
                     KSEFA"
     gene            5729..7396
                     /locus_tag="I9760_000605"
     CDS             5729..7396
                     /locus_tag="I9760_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2552 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042395.1"
                     /translation="MKEIVSLLEQDQIDFANHKRPEVIMVLRRKFRTQKDWIITSFNQ
                     YQFTKEESQKVQQAILNGDLRITDLLNRPFSHYFWSFLTFKWGEILSGGKRLSKQEVL
                     QIASCSSNGQEFARTPTQNRNRLIKGVPLVIGKVVTSMALCMVFCLGLLSTLGAPFGL
                     SLGLAIILSLCNGIVCLLSRGKAMLDSAGFQPQKQKTFKTLEENIKEKTIFDRGQKLF
                     FGVVTLLACISAGISGYAGLVGLLTFLGAGMLISNPLVLILTIGLSVVAAVSFYSFQA
                     IGIRENYTKFKNLFIEDYQKPYGLLRCVLLGMVSTVFLAVYATLTWFTTVSGVNKLFN
                     ALGATPDTLLAHFIAMICTSTTMVVNTFSQVYKVFNPKTYVDLKEKFESIYQPPEENL
                     TRAQKIKHGAINMLKLFALIGDSLAYSVAGGIRSGIHIGETLGETIGAKFALTITMAI
                     LSPVILVFVSIAFTWPTVFDRKKSTPCPSEMDKPLIEKMSHSHTSDVLQNNTLLPTSE
                     KLSKPDDSNIRQAGLRFFDVGLSEERTHKPNSNPTLESRAVLTSPSP"
     gene            complement(7432..8277)
                     /locus_tag="I9760_000606"
     CDS             complement(7432..8277)
                     /locus_tag="I9760_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HAT5042396.1"
                     /translation="MTITTITSPQETRYIDLSSCYFYYSSRILAFVTWLSCGLFGIYI
                     LFFYANSLISDSASRWNNVLPNLYIKSHTASTLGIAIHFTAGGIILAMGFLQLLSTIR
                     HYAPGIHRWLGRIYVIAAALTGIGGLTFIASTGTIGGLVMDIGFGLYGLLMILCALQA
                     YRYARQLKFDNHQAWAIRLFALAIGSWLYRMEYGFWFLMTNKLWHTQTFQGPFDFVMA
                     FFFYLPNLLIAEAYLRGRSAQVSQFSKLIAGTVINLASGLLAVATYFFAKQYWLPAIW
                     GHSIR"
     gene            8405..8974
                     /locus_tag="I9760_000607"
     CDS             8405..8974
                     /locus_tag="I9760_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042397.1"
                     /translation="MNLMLRKWIGLIAAFLFLNTGFVQASATPIICDQEYTLCTSARC
                     IPMPGNPAKAVCDCVVEKGKSAGYKTCKERKPIRDRYKVTSLVSTFSFEQFAFKRKMN
                     CSEGLPWTNCLDMPCTVDPQNSKRALCLCTIENTQAFFTFGGDCNTNTCATGFWSGAT
                     QENSIILRKALMQEMRLKPKALLKDCSAK"
     gene            9259..10014
                     /locus_tag="I9760_000608"
     CDS             9259..10014
                     /locus_tag="I9760_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5042398.1"
                     /translation="MLLTNRIDLRSYHTESCSHTHDFAQLVLPVSGSMELEVGHYSGV
                     VNVDIGVYIAPNEQHCFAGSQKNLFLVIDATTQNSLRDEAFESNILNLTASTKKLIQF
                     THHYLAHNERDFFTDSLINQLLFHFVANSFSPEPDPVVIRAKQWIDLYFADSVDVSRV
                     AKHCYLSISQLQRRFKQMLGCGIAEYWRMKKLLHAKKLLSQKNCSIEAIAFEVGYENL
                     PAFSRRFSKVFGESPSQWRAKALTAKKMREIDK"
     gene            10080..10994
                     /locus_tag="I9760_000609"
     CDS             10080..10994
                     /locus_tag="I9760_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAT5042399.1"
                     /translation="MVIDVKKSNTYFIQGMLFLIFAQIMVGINIVFSKYVLSSIPIIY
                     ILALRFTLAAVILLLLHWLTPAKKLPVYFYFLQLKRRDWFFIFAQALSAGVLFNFLIL
                     WGLQYTDANVAGIITSALPAIIAIMSWLILGEKISGKKAICVGFATLGLVVIACDKLG
                     DINVSHSFLGDALVLTSLLPEAMYYILSKMHVNSLPVFLISSLLNGINAILLLFCLSF
                     STWDSFTIHTLDWLILIILGLSSGLFYVFWYFGCQTVDGVMASLSTAVMPLATVTIAW
                     IFLGEQLTIGQIIGMGMVILSITVYAKR"
     gene            11004..11339
                     /locus_tag="I9760_000610"
     CDS             11004..11339
                     /locus_tag="I9760_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HAT5042400.1"
                     /translation="MTISYEEFEKVDLRSGTVVKAESFLRARKPAYKVWVDFGADIGV
                     LQTSAQITVHYTPEELIGRMVIGCVNLGEKNIAGFLSQFLLLGFSDEKGAICLASVDP
                     HVPNGKKMH"
     gene            11514..12923
                     /locus_tag="I9760_000611"
     CDS             11514..12923
                     /locus_tag="I9760_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2546 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042401.1"
                     /translation="METKMSITVKSLDFDQCISNRKYKESLQTNDGRKVWDANSLFDA
                     NKEILGKNNNGDPIHVFIGSNRQNLKADLINLNAGAATLFIPVAQELCNIMGATFHPL
                     LVPDLICENATIGDTFHSALQVIKGLNDLNSLNSKSLAELVKSALSGQLNSLHCISDE
                     SKFLMLYSQIQYMAQQYPDEKINFEFYDDKEDILKPLYDIFSKNPDLIPANVTLNIKR
                     YLNGNLMETDFSPILGLGSQQENYQNIVKWIHKQSSSHLKSGNCCQVLEMDNEKIARY
                     CRFGKDETRLKLLDSLENLAKHQVGQKDQKMDGFIKESYEKMGSSKDMDSITLQQSFE
                     EISSAIKVTEAINKVIANYRKEAKCLFSVGMNAKADRIEKALLNVPVEDRGKIFSNDK
                     VSPELIAIRAALASHRYFGKRGNVYYKDEARTVIDENKAATTYNNLRKQFANLRTQSH
                     ADAQVELEHSSEVSRALKL"
     gene            complement(13924..14214)
                     /locus_tag="I9760_000612"
     CDS             complement(13924..14214)
                     /locus_tag="I9760_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042402.1"
                     /translation="MSIISNHDITFSSTQKDLIEKQQKNNIKNQHIADVKASKGGETS
                     EKLIVFGAKTTALAACGIALLFPLTAPMIIPIAQVSGAVALVIITRKFIKSS"
     gene            complement(14591..15964)
                     /locus_tag="I9760_000613"
     CDS             complement(14591..15964)
                     /locus_tag="I9760_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAT5042403.1"
                     /translation="MFQSILIIALMTFSFITQAALPADPIQRCLDIRRLVDITSRQKM
                     AAKEPGVLRFKFHKISASELPLDNPVGNMDEVIQALNNQIENCNNSYGEFQTVTIAGH
                     QFKRQEWCLNTNKKMLTLAKAAHGDFKKFLSSIKTEFDWYKSDGWPENHAGFKKGEFQ
                     FTAYYAPAAVEARSKRGGAFLYPLYSNPGVVSVALESKKYNLKTPLCGVDPLSKVMRG
                     FCLKNADGTYSVAPDREEIEHGALNPKYIIGYLKDPNDSAFLMLQGSGSLLLDGKLFH
                     INYDGANGRPRTMLGRIVQCAQDPTCGGNLDTMERCAKDPKCHDEAKLRCNVSKKIRQ
                     SAASEKLIRQYLNNLTPDKADNLRNRDQSYVFFVKEDGGPYGSENISLTPHVSCATDH
                     RVIPVGMSFIYHCKKATSWCVAQDAGGAIIGAHVDVYKGEGNQAGVEANQLNHTGTLF
                     VALPKRK"
     gene            16482..17147
                     /locus_tag="I9760_000614"
     CDS             16482..17147
                     /locus_tag="I9760_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042404.1"
                     /translation="MTLILKMKKFKLMAKEQCFAFIKNAVNVGMDEVIYSNGTNKTFI
                     TDGLATCAAVAIYGSDSSCSAAFTHMSSEASDKDDKRKETILNEMLEYVLKSNSLSDV
                     KLIISPSRIQEKHLISFILEWVKQKKIAYSLLETGGDSAVFNIDMDSRVLMLSTSLQL
                     KQDVSDKTWHGHGIIIGQSEGIPVIEKHKKHFVFFAEDISSQITKFSDPPKTAGLAAA
                     VSA"
     gene            17280..17705
                     /locus_tag="I9760_000615"
     CDS             17280..17705
                     /locus_tag="I9760_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042405.1"
                     /translation="MRRHFIIIVLGIFQFGCIPLAEHHNKISPATAHEEYHEVNFSNL
                     YEKNRSFPALETGIYAILDGILISSSQNTHLGKKQSKLLEEYVRQNQLFCQKGFDQDW
                     MNQLKQNLDRLEKTENNRIASAVNKKMQTLFAEQQCNAL"
     gene            17969..18802
                     /locus_tag="I9760_000616"
     CDS             17969..18802
                     /locus_tag="I9760_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2541 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042406.1"
                     /translation="MLMEFEDWFLEQINNGKKCILIGENHYSYASEDCLDCILKLQKQ
                     GKINKKIVFFDENLSALDNLESYTVESLSKLNTVEPSLIALIKNNVEVYGLEDNVSNP
                     FINLKSMKKVTARIKDLGLQIGNRIFNDFDHCLYWAYHLYGKSLVRVQRGNEGFCNQI
                     LHKMDEDTIVIARVGAKHIPTVRKDGVVIEEGMLQRLGKDMASACFITHFPKNRTYSE
                     QKTYQPESDAFGSYDSIECCMVQKKMIHQPGLTLWAEQDKQRKDTNDYKDTKKHRCTI
                     M"
     gene            18918..19301
                     /gene="mscL"
                     /locus_tag="I9760_000617"
     CDS             18918..19301
                     /gene="mscL"
                     /locus_tag="I9760_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAT5042407.1"
                     /translation="MSLLKEFKEFAMRGNVMDLAVAVVMGVAFNKIVTALVDGIIMPC
                     VGLLLGGVNIAGLSFTVGDAQIKWGNFLQNVIDFIIVAFAIFILIKLINLLQRKKANE
                     PEPVTLEIQLLTEIRDLLARNSSKI"
     gene            19513..19920
                     /locus_tag="I9760_000618"
     CDS             19513..19920
                     /locus_tag="I9760_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2539 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042408.1"
                     /translation="MKLIINKKNRAESMALDVEATTTVNELLLILYTHRSKRYEPLSF
                     SEYQKFISVYQCLLYGDQFINKLLPGTNLADYQLEDPCHLTWEEFTEKQCLLADAETS
                     RILLWQANKKASKPEIEKDLMSVASSDAMNPDM"
     gene            20571..21917
                     /locus_tag="I9760_000619"
     CDS             20571..21917
                     /locus_tag="I9760_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2538 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042409.1"
                     /translation="MKTKYEISQDKTEFLAKEQSSSYPGYQVSVLDLEKMVKHYQEKY
                     GIRLIINGTTPKYQALIKDRQVNFEQQKQQFLELKYAKFLQIFFQPPNLNGANSPFSI
                     NKYMGAFIGFYEEIYNKVLPFLDAKGKVISGLSLEELRQLNEACQELSCKGILDATID
                     EFIERNSDYMGLTARESASEMKDICDELQEGEVLGYFFTGQRTSGRCHFDLYICLPGK
                     AIRPIFYNTALIRYHDLGGMFHLNFPFVEGNFFTPDLLKLYSAMDLQQLIPQADRTSC
                     GTLTMMYAKELLKDDARGLKEFTLSFTYYNEKGEKEYFFLPSPQVLRYSQISLYNEAL
                     KAILSHENDGQAGLVRKGAKKYMFHTIEKILIQSFKIALEKEDADVLEENQKIWDILP
                     SFQEKWQEAYKEMVAIRDVMHQGVNKYLLYSTHRMSHIASDESINNEADADRLILR"
     gene            complement(22041..23060)
                     /locus_tag="I9760_000620"
     CDS             complement(22041..23060)
                     /locus_tag="I9760_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT5042410.1"
                     /translation="MIIDFETHFITKACIDYLTQRQEVPKLVPELNGAYTMCFTPDVS
                     LFHTSALMEELLSLNEQRLAIMDQAGVTIQVLSLTTINGIDSCPGDEIKSTALAREVN
                     DQLYSAIQRHPERFKGFASISPYDVKEGVKELERAISQLGFVGWLTHSNFGEDNYLDD
                     KTYWPLLEAAEGLNIPIYLHPNVPIMREFGKYGFALGGSALGFEFDTALCLMRMILGG
                     VFDAFPKLKIMLGHLGETMPFLMERLDHLYRIPDLKAYRPSIQRIPSEVLRQNVYITT
                     SGRFFVPALRYVLEVMGEDRVLFASDYPMESLLDATRFIQDSDLSNQTKQKIFSINAK
                     NLNLI"
     gene            complement(23285..24241)
                     /locus_tag="I9760_000621"
     CDS             complement(23285..24241)
                     /locus_tag="I9760_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HAT5042411.1"
                     /translation="MSTVRFNNQSFALTPDESLLQCFLRHGVEYPNSCQAGICQSCLI
                     KAKDGEINPDWQEGLPETLKSQGYFLACLAKPSTSLYVASPDNAECETQAQIMSLQRL
                     NHNVIQVKLKVEHPAPWLPGQYLSLINPEGTMRNYSIANIPADDGYIELHIKIYPQGK
                     MGQWLLQRAAINTFIAIRGPFGQCYYHNPHNLAFDILLAGTGTGLAPLIGIIRSALTQ
                     KHEGTITLVHGGVTDEDIYYKEELEMLSLLFSNFRYDPCVLQSQGLYPEGSIEKRVLT
                     HLHSPNTTKVYLCGPKETTNKLKTRIFLAGVPSTNIYSDVFL"
     gene            complement(24238..24612)
                     /locus_tag="I9760_000622"
     CDS             complement(24238..24612)
                     /locus_tag="I9760_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HAT5042412.1"
                     /translation="MTESLFERLGGQNAVNMAVDIFYRKMLMDDRVNYFFDDVDMEQQ
                     ILKQKGFLTMVFGGPNQYTGKSMREGHQHLLARGLNDSHVDIVIEHLGETLKELGANE
                     EDIQKVAAIANSVRGDVLGRSS"
     gene            24693..25124
                     /locus_tag="I9760_000623"
     CDS             24693..25124
                     /locus_tag="I9760_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAT5042413.1"
                     /translation="MQLTQFTDYSLRALIYIALKKGSCTIKDITDAYTISNNHLIKII
                     HNLSKMGLIKTIRGKHGGILMAASPETINLGQLIMELEPNFDLVPCFNKEKANCCIAP
                     VCKLKSVLYEAQRAFLQILEQYTLADVLHNPSELSALLRIS"
     gene            complement(25157..26053)
                     /gene="tehB"
                     /locus_tag="I9760_000624"
     CDS             complement(25157..26053)
                     /gene="tehB"
                     /locus_tag="I9760_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HAT5042414.1"
                     /translation="MPREYDNLICYKQLSIDDHGKLKFFLEKHSTKEGTWGKLSLNEG
                     EIDFIFQNGLGQELSRIRLDKNNRQLSIPPASWHKIIPISASFDAQLSFYCKPHRYFN
                     KKYGLTEVHRDLLYVYHTYLSHLGSLTTLDVGCGSGRNLLYLAQLGHRLTGIDINQTA
                     LENIQIIAQKENLSPLNILSQDLNQPQSLAPDHYDFVCSTVTLQFLNASRIPSLLIEM
                     QEATKKNGYHFLVFPVQSELYSLPSSFTFLPKQQELYHFYQDSGWSILEYKESVGHLH
                     KQDELGRPVQGLFALLLAQKVI"
     gene            complement(26355..27521)
                     /locus_tag="I9760_000625"
     CDS             complement(26355..27521)
                     /locus_tag="I9760_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAT5042415.1"
                     /translation="MNHCEKKNLPKDSATVAINSLAQQKINAGIKIHNLSAGEPKLPP
                     HPSIINAVTQAMEQGHTLYPPIMGIPELRHMGCEWMYRSYDCSFQNEQCLVVNGGKLG
                     IYLLMQLLLQPNDEVIIPSPYWVSYPAMVSLFAGTPVPVETTEAEEWKLTPQALKKAC
                     SAKSKILILNNASNPTGALYTQSELIHLLQVAEEHNLLVISDEVYSELTYDGHHYVSC
                     GSLPQFRERVIIIQSCSKNFSMTGWRVGFIFAPEIFIKPLSALVSQSTSGVTTLSQWA
                     AVAALQEVNQVNIWVQQNMEKRRNCLLRTLHDYLGLTITPPISSLYVFLSLKDIGCKN
                     QNSEEFCKQALDKANVAMVPGIAFGKEGYVRLSFGGNEDDLKSGIYALAQWLHR"
     gene            complement(27521..27781)
                     /locus_tag="I9760_000626"
     CDS             complement(27521..27781)
                     /locus_tag="I9760_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAT5042416.1"
                     /translation="MSTIEELRKKIEQTDAYIIEKLAQRQELSKQIGALKAKEGKKII
                     DRLREKKLFEYYDYLSRQYHLQQDFVNQLFKIIISNSKKVQK"
     gene            complement(27816..28400)
                     /locus_tag="I9760_000627"
     CDS             complement(27816..28400)
                     /locus_tag="I9760_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAT5042417.1"
                     /translation="MKNLVEEKEAIRFGVSGDIGSFSEEAALLYAKEKDINVHLIHLL
                     DMEGVLNAIENETIDLGILPVVNLLGGLVKPAFQAMGKYSFTPIDEIWHDINQCLLVK
                     PNTQHHKITHIVSHPQGLAQCKKFLRHKFKDAKKIEWIDTAKAARDLAEGILPPHTAV
                     IASARCAPLYGLQMRARNIQDSYQNKTAFILVKK"
     gene            complement(28397..29440)
                     /locus_tag="I9760_000628"
     CDS             complement(28397..29440)
                     /locus_tag="I9760_000628"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAT5042418.1"
                     /translation="MSYSILKKLPPIEKIIRELPLSHPAYLQIAQDRQEVKAILEGRD
                     KRLLMIVGPCSAWPKKAVLEYAERLVQLNSKVKHALKLIMRVYIQKPRTTKGWTGPVN
                     QPDLFSTPDIEAGIKYTRDMMIKVIQMGLPIADEALFTHNAKGFLELLSWVAIGARSS
                     EDQEHRIFASALDCAVGLKNPTHGSLAVGVNSLVASQHPHVAVFDGYEVQTHGNHHAH
                     LVLRGSNQAPNYSVSHLEEVMYYMGMHQIVNPSVIIDVSHDNCLVNGKKNHQLQPEIA
                     FNILESIKNRPDLKQLVKGFMVESFIKEGNQKLDSINPDNIDLSGLSVTDPCLSWEKT
                     ESFLLELAEREAL"
     gene            29962..30213
                     /locus_tag="I9760_000629"
     CDS             29962..30213
                     /locus_tag="I9760_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042419.1"
                     /translation="MSICWDLIINHNTLLQTNFTGHRVQMNDSESSPKGILFVSNKLF
                     IDLLTYITEYQSITRKELMNLSNNEIKPRRKSNKRLLMW"
     gene            30207..31925
                     /gene="lem27"
                     /locus_tag="I9760_000630"
     CDS             30207..31925
                     /gene="lem27"
                     /locus_tag="I9760_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem27"
                     /protein_id="HAT5042420.1"
                     /translation="MVRVLNLQKGVNMFNVDNSGKGNCLYYAYSISLMYYLRAKNNVR
                     ITEDIFNKLGLKEEDRARLRKLLSKDPDRAFTRDEIKTIIEPILGRATRDLAAEHTKV
                     EFKSSPHDTPLFSSLHYAVEFGFKRSLQINESELTLLIDNDFSNPDYTEAEIYKVSGL
                     LDALQEYILTRTPSVIEEFNRQWENKKQELKEQSLTEKEIQIHQATILDNILRKETID
                     FLLADNEKHLDEYREHLRREFVWGSEETLMVLHRAIQGERMVRNHEGRIEPVYDHEII
                     LHVHRNGASPPYQAGSPEMILNNEGNVHWTSIIPDAIFTSKLTDKEEKLLEMLERMQS
                     EYGSKELGVEKYSLISDWLSDLMKQIELIKTSPTAVTKQKEIELFFQLLSKATPKLAS
                     EPALRMSLGTLFSNFLECIPALMVENKLSTGLAKFGLYSPVISQKKEDLSLIKSSEEK
                     ANSEQPQKGVLSQFSKHTLLKVSEDVSPKPYPTELNPYVRKNKQSGPRVARALTQMYQ
                     KGLEGDERYTPEKCKDIAQRYIEYSKSHKRNFILDDVILFVQEMDKIIKQQESISENS
                     SKRAAM"
BASE COUNT        10100 a         5705 c         6207 g         9949 t
ORIGIN      
        1 aaagcaaatg gggttgttac aataaagaag atatatatac taaatccttt gaaaaaggct
       61 tgaaaaatct tacaattatc tgggatttgt tagcgatgcg ttcctaacaa acccagagta
      121 aaatcaatat attataattc aaagcctgaa ttggttaaat tttgatactc tatatcttct
      181 aagaagccat ttatttttct tataatatta gcttcgcttg catgcaaaaa tttattctga
      241 tattgatgaa taaaatcata taattccttg ggtatatctt gagattcgtt ttcatccatg
      301 atccgattta gttcattcat cattttttgt attgttgtaa aacggcctat tgtgttttta
      361 aaaattatat tactgcgata atgagaaaat tcaccaggaa gattctctaa taattcctgc
      421 aaatcctttt tgatatcccg ctgaatattt aattgtgtaa gatcagaatt gtttgtgctt
      481 ttagttggat tttgaaaaaa gcagagatta ttaatatttg agtgattctt tattgaggat
      541 tgaggtaatt taagcttcgg cgcgttaatg gacgcaagtt gcttaaattg gtttattgcg
      601 gaatcaatct ctattctatc ctcttgtctt tccgctaaca tttgtttcaa aaatggctct
      661 acccatttaa cttgtgcggg caaattaagg ttttttgctg catttcggct tttattaagg
      721 gggttattac tcaaatagtt tgtagcactt tcgccccata agaaggcaat cactcttccc
      781 attgaaaaaa tatcagcttt actatcgtgc tttaagccaa taaacatttc aggcgctgcc
      841 caactttcac ttccaggaaa taggttatcg tcttcgcctg catttttggc aagtccgtaa
      901 tcgaaaatat tcacttcaac agggtcaacc actttaacat gaatattctc aggctttata
      961 tctcgatgaa taattccttt atcaatcacc tgatttttca aagccaataa taaagcatag
     1021 gttatcttaa ttcgttgacc ctctgtaaga taatgaggat tagccattaa tttattgaag
     1081 atacgattta aatccatccc tggcattcgt ttcatcgtta gataacattt attattattg
     1141 taaacaggtg gttttatact taagtgccct gcttttcgac aaaactcaaa ctctttttca
     1201 attaaatttt ctatatttaa gttggtgtat tttattttaa gtttatttaa attaaatttc
     1261 tttgttacaa acttcttatg atcagtagat tttttagcaa atccagtttc accttgccga
     1321 tacagcgtaa caggataaac agaagcgaac ccaccttttc ctattggttc tttgttcata
     1381 actgaaaaaa gatacccttg ttttatctct cctttcttat caaattgatt tcggggtccg
     1441 ccgataattt ttccagtgtc cggaagtaaa tccttttctt gtaattcatt gattatttct
     1501 tttatatttc tatcaaacat attttaatta ctcccaaaat agttattcat gtatttgcta
     1561 actcgtctaa attgaaataa ttagacatat tttaaccatt ttgatctata aaataactgt
     1621 cgaaattgaa cagttatggc acttattttt tacctataat cacgccgaca acttgtttct
     1681 gtttctatta cttaagctac aggatttaat aaatgaaaat tactggagca attaagatga
     1741 tccaaacaca tggacgagca gacagatttg tgaaaaagca actaccaaat gattggtttt
     1801 tagcactcga aattactgat taggaagaaa tactgataat gatcttgaac agcaaaagat
     1861 tgataccgca ttattgatta attgtaggtt ttgtcatagg gttgaaccta aaataatgga
     1921 tgaaaaaaat ttaaaaaaac ttcatttaca cctgggatta ctctgctcct attcttttta
     1981 cacttagttt attcctattt cactgctaaa aaatgacagt tatttctatt gctctaacgg
     2041 gctataattg taaaaaattt aatctttatg atcttgcgat tgttacatta aattatttaa
     2101 gattaatctg gagttaacca tggccaaaga actaattttt attgattggg atgatacatt
     2161 agtcaataga gagaacgcag aagattttga atcggtaaca gacgaaaaca atactattta
     2221 tcaaatgtct cctatgttta ttggtgcagt agaagcgctg aaacaacttc aagaaaaata
     2281 tcatgttgtg atcgtcagtt cacgaggaga cttaaatagg ttaagacagc tccaattaca
     2341 aagttatgat ctagagtctt caattatgcc attagaaaat ttcacatttg aagacaatga
     2401 taatcgttta ttatttacaa ggtatggtga aacaaacttt cctgataaag caagtttcat
     2461 tttgtatatc caaaaaatga gcggtaataa gataaaagta tttatcgatg atgaccatac
     2521 agaaatagaa gccgcacagt caaaaggaat acccacaata catgcgactc ataataaata
     2581 tctgcaaaat acagaacaat ttttttcagg attaattcat caagtagata acatggaact
     2641 ttcgcatatt tttaataaag tagcagctga aatacgtaac aattctcgtt caatttcctg
     2701 gggaaggcaa attgctggtt tcttttcagt tcacctgcct atagcacaga gtttagaaaa
     2761 tttggcatct aaattacaaa gaaaatctgc taatgaggtt agcgaagaat ttgatcaata
     2821 ccttaagaaa attgcttatg atccaaataa aaaaggggat atagccgtaa gtttaactaa
     2881 agctaaaaca gcaatagata aacatatatc agccccgatt gaaatcaaag aagatcaacc
     2941 atttctcaat agaatgattt aacaattttc tcctttctcc attatccagg tgccttggta
     3001 tgtcctagaa gctactgcca ttcactttag cttcctcaat cgacatcccg gggcttgtcc
     3061 tcgggattta aaatttgtga gatcctgcaa acaaatctcg agattcaggt atatgtatcg
     3121 atatctaaag tcaatagcaa tgatccaagg agaaatacat tgatttctgt aataatcaat
     3181 atttcaatca tccagttgcg atttaatttt atgctgttga cttattgaat aatgattctc
     3241 tacatcattt ttatgaagtt taaaattcag agaatagcct ttcacttgca agtacttaat
     3301 ttactatgaa ttattttaaa gtctaaatga gtatgaaatt cattgatgaa taggacgatt
     3361 tcttaatttt agattattta ataagttaac caggtaaatc aggttattag cttgagtaag
     3421 ctttacttct tagcattttc aaacgacttc aatttcaaca ggagcaatgc cttttatacc
     3481 gagtttttta gcggcagcaa aggataaatc aattagcctg tttgataaaa aaggacctct
     3541 atcgttaatt cgaaccacaa ccgatcggcc attattcagg tttttgacac gtactcgtgt
     3601 agcaattggg agcgttggat gagcggctgt catcgcatac atgttgtaac gctctccggt
     3661 cgatgttttt tggtgccgag cgtgtgaccc ataccaggaa gcaattcctt ttgctttgta
     3721 gtgtttggca gacttcatca catgatatgt tttccctctg actacatagt gatccggacc
     3781 atgagattgc tgatgcgtac aagcaaccag tagtatgggg agtaaaccta taatgagtcg
     3841 tttaatggtc aacaaaatcg aataataatt cataaagtta tcttctactt ccgctacaga
     3901 ttttaaatat acgtaattca tagatcacga gaatcaaaga tatttcttgc aaagaaaatc
     3961 gtcaataggt aacacatttt tatcatttgc tttcatcatt gatttgattg attatacatt
     4021 ttttggatga atatgtacca acaaattttt ctgtataacc tcttgtccta tcacaacaag
     4081 gatatctcta taccccaggg gcattcatgt ggaggtagca agagcaggtc gggccaaggc
     4141 aggtccgacc tactttttct ggattaccct gggaagccaa tcactaagca aattcggatt
     4201 tgcctgaaaa ttctgattga cgatactttg gattaacttt cacatgccag agcggcagca
     4261 tcaaaagtaa tggaatcaag cagcaaacaa acaatgtgag gaagaaatta tcccagccgt
     4321 aatttttgat aattgcaccc aaaggagcac cggccagaac agcacctaaa caataagcaa
     4381 aaaatccggc aaagccagtt gcagtcgccg ctgctttttt atgtgctaat tccgcacagg
     4441 ccataccgat catcatttgt ggtccaaaga taaaaaaacc aaagaaaaaa agaaacaaag
     4501 catcgaggaa cggtgtatat gccctggtta acgtaaacag caataaagtg gcaaatacgc
     4561 ctgcagtaaa aagaacgttg attggattgc gtttaccttg aaatatttta tcagaagacc
     4621 agccggcaac cagatttcca aaaaagccac caacttcaaa ccatatgaca caactgcctg
     4681 cagtgattaa ggaatactct ttcacttccg ttaaatacaa catgctccaa tcattaatgg
     4741 ccgttcggac aatgtaaata aacaaataag aaatggacag tatccaaata tattggttgc
     4801 acaacacata attccaaaga atttctttga ctgagagttc tgttttctcc tggtggtgat
     4861 ttgtggaacc agaaaaatct tcatgatatt gctctatcgc gggtaaacca agtgattgtg
     4921 gggtatctct taaacgattg attaaaaaaa cacctcctaa aatgcaaatg atcccaggca
     4981 caaacatggc tgaacgccag ccgaagtatt gcgcgcacac agcaacaatc aaaggaatta
     5041 atgcaccacc aacgttgtgt gatgtattcc aaagactcca ccaacggcca cgctccgatt
     5101 gagaatacca atgtgttaat aatttagtac atcctggcca gccccaccct tgaaaccaac
     5161 cattcaatcc ccaaaaaata gcgaataacc accaggtaga agacaatcca aacaaaatat
     5221 taaaaacacc tgtcaaaatc agtccgatcg ccattaaata acgaggatta gatttatcac
     5281 caagaattcc actcaaaaat ttgctgatac cataacttaa actcaaaatg ctggcgatca
     5341 ttcccagttc agttttagtc atccccaagg tgctttgtaa cgcaggcatc acgaaggtaa
     5401 aactctttcg cgtaaaataa aacaacgcat accctatata cattgcataa aaggtgcgaa
     5461 tacgccaata acgatattgc cgtttaacaa cgtttttatc ctgaatttca tcaagatatg
     5521 gagcaggttt tagaaaactc aataatgcca tgattgtcca tgatcatgaa aaaaacatga
     5581 ttagaatgta actcgcaaag tatgtcaaat ctttctagtg aaaatttaac aggatcatac
     5641 ctattgatga ttgaaataaa aatttaatgg aattcttgtg aatttataaa taagctacgc
     5701 agtttgtatt aaaaaaggat ttattgtaat gaaagaaata gttagtttat tagaacaaga
     5761 tcaaatcgac tttgctaatc ataaacgtcc cgaagtgata atggttttaa gaagaaaatt
     5821 tcgtactcaa aaagattgga ttattacctc tttcaaccaa tatcaattta ctaaagaaga
     5881 atcgcaaaaa gttcaacaag ccattctaaa cggggatctt cgcataacag atcttcttaa
     5941 tcgtcctttt tctcactatt tttggagttt ccttaccttt aagtggggag agatactttc
     6001 aggtggaaaa agattatcta agcaggaagt cttacagatt gctagctgct ctagcaatgg
     6061 ccaggaattt gcgagaacac cgactcaaaa tagaaacagg ctgataaaag gagtgccatt
     6121 ggtcattggc aaagtcgtta cctccatggc gctgtgtatg gtattttgcc tgggattatt
     6181 aagtacttta ggagcgcctt ttggccttag cctcggtttg gcgataatac tttccttatg
     6241 taatggaata gtttgcttat taagccgcgg aaaagccatg cttgatagtg ccggctttca
     6301 gcctcaaaaa caaaaaacgt ttaagaccct tgaagaaaat ataaaagaaa aaacgatatt
     6361 cgatagaggc cagaaattat tttttggtgt agtgaccttg ttagcgtgca tttctgcggg
     6421 catctctgga tatgctggtc tcgtaggact tctcacgttt ttgggggcgg gcatgctaat
     6481 ctctaaccct ctggttttga tacttacaat tgggctgtct gtggttgcag cggtctcttt
     6541 ttattcgttt caagctatcg gcatacgtga aaattataca aaatttaaaa atctttttat
     6601 tgaagattac caaaaaccct atggtctttt gcgatgtgtt ttattaggaa tggtaagtac
     6661 ggtctttttg gctgtttatg cgacgctaac ttggtttaca acggtttcag gggttaataa
     6721 gcttttcaat gccttaggtg cgactcctga taccctttta gcacacttta ttgccatgat
     6781 atgtacttca acaacgatgg ttgtcaatac tttttcacaa gtatataaag tctttaaccc
     6841 gaaaacgtat gtcgatctaa aagaaaaatt tgaatcaata taccaaccac cggaagaaaa
     6901 cctaacaaga gcgcaaaaga taaagcacgg agctatcaac atgctcaaat tgtttgcatt
     6961 gataggtgat tctcttgctt attctgttgc tggtggaatc cgaagcggga ttcatattgg
     7021 tgaaacgctg ggagaaacaa taggggcaaa atttgcatta accattacca tggcaatatt
     7081 gtctccggtc attttagtat ttgtatcgat agcttttacc tggcccaccg tttttgatag
     7141 gaaaaaatcg actccatgtc cttcagagat ggacaagcca ttgatagaga aaatgagtca
     7201 cagccatact tcagatgtgc tgcaaaataa tactctatta ccaacatcag aaaaattatc
     7261 caaacctgat gattcaaata tcaggcaagc tgggctaaga ttttttgatg tgggcttgag
     7321 cgaagagcgt actcataagc ccaattctaa ccctacttta gagagcaggg ctgttttgac
     7381 atccccctcg ccataagtga tgaggttttt aaagcaacca ggctaagctt gctatctaat
     7441 gctatgtccc caaattgctg gtaaccaata ttgtttagcg aaaaaatagg tagctactgc
     7501 taataaaccg ctagccaagt ttatcacggt tccagcaatg agtttggaaa actgactaac
     7561 ttgtgctgag cggcctcgta aataggcttc agcaatcaac aaattgggca gatagaagaa
     7621 gaatgccatc acaaaatcaa aagggccttg aaatgtttgg gtatgccata gtttatttgt
     7681 catgagaaac cagaagccgt attccatcct gtagagccat gagccgatcg caagtgcaaa
     7741 caaacgaata gcccaagcct gatggttatc gaattttaat tgtctagcat aacggtaagc
     7801 ctgtaaggca cacaaaatca ttaataatcc ataaagacca aaaccaatat ccattaccag
     7861 cccaccgata gttccggttg aagcgatgaa agtcaatccg cctattcctg taagagcagc
     7921 agcaatgaca tagatgcgcc ctaaccagcg gtgtatcccg ggtgcgtaat gacgaatggt
     7981 gctgagtaac tgtaaaaatc ccattgcaag aataataccg cctgcagtaa agtgaatggc
     8041 aatacctaaa gttgatgcgg tgtgcgattt gatataaaga ttaggtaata cattgttcca
     8101 acgagaggca gagtcggata ttagagagtt tgcgtaaaag aaaagtatgt aaattccgaa
     8161 taagccgcaa ctaagccatg taacgaaagc aagaatgcga gaagagtaat aaaaataaca
     8221 agaagataga tcaatgtatc tcgtttcttg aggagaagtt atagtagtta ttgtcatgtg
     8281 ctcaataatt aatcatagtg ggaccagagt tagtctacaa tgtccagtca cctatttaaa
     8341 gctcatacga ttcgataata gtataaatat gctgctataa ataaaaaata aagagatctg
     8401 aaaaatgaac ttgatgctta gaaaatggat tggattaata gctgcttttt tatttctcaa
     8461 tacggggttt gtacaggcct ctgcaacgcc aattatatgt gatcaggagt atacgctttg
     8521 cacttccgct cgttgtattc ccatgcctgg aaatccggcc aaagcagttt gtgattgcgt
     8581 agtagaaaaa ggtaaaagtg ccggttataa aacatgtaaa gagagaaagc ctattagaga
     8641 cagatacaaa gtgacgtcac ttgtttcaac tttttcattt gagcaatttg catttaaaag
     8701 aaagatgaac tgctcagaag gattaccttg gacaaattgc ctagacatgc catgcacggt
     8761 tgatccacaa aattcaaaac gtgctttgtg tctttgtacg atagagaaca cccaagcttt
     8821 ttttactttt ggcggtgatt gtaatacgaa tacctgtgct accggctttt ggtctggtgc
     8881 aacccaagag aatagcatca ttcttcgtaa ggcattaatg caagaaatgc gtttgaaacc
     8941 caaggcacta ctcaaggatt gctctgctaa atgaggataa gcaaactaga gggagttgat
     9001 taaaatttag attgattcaa tgaatctgtg agatgctcac tacaacagag tctagccctg
     9061 ggcgatcccg ttgcctattt tcaataaact ctacccaggc gtttaaagcc tttgttagtc
     9121 ctgacctatc cgtgtgataa aaaactcaag ctgatccaaa taacgatatc taatttcctg
     9181 actacccctc tcaatggttt caaatcaagt atgatataat ctgtttcttt taaaggtaaa
     9241 tgctcgcttg ggagcaatat gttgctgact aatcgtattg atcttcgttc ttaccatact
     9301 gaaagttgca gtcacactca cgattttgcg caattggtac tgcccgtctc gggttctatg
     9361 gagctagagg taggtcacta ctccggtgtc gttaatgttg atatcggagt atacattgcg
     9421 cctaatgaac aacattgttt tgccggaagt caaaaaaatc tgtttttagt gattgatgca
     9481 actactcaaa actctcttcg tgacgaagct tttgagtcta atattctcaa tttaaccgcc
     9541 agtactaaaa agctcatcca atttacccac cattatttag cacataatga aagagatttt
     9601 tttacagact ctttaatcaa tcaattattg tttcactttg tggcaaattc tttttcaccc
     9661 gaacctgatc cggttgttat cagagcaaaa caatggattg atctttattt tgcagattct
     9721 gtggatgtga gcagggtagc gaaacattgt tatttaagca ttagtcaatt gcagcgccgt
     9781 tttaaacaga tgttaggctg tggtattgcc gaatattggc ggatgaaaaa attactccat
     9841 gctaaaaaat tgttatctca gaaaaattgt tccattgaag caatcgcatt tgaagtaggt
     9901 tatgaaaact tgcccgcatt tagtcgccgt tttagtaaag tattcggtga atccccttct
     9961 caatggagag ccaaagcgtt aacagcaaag aaaatgcgtg aaatagataa ataagctctg
    10021 gttttaaatt gatagactac gaattcattt gatggtatcg attattaaat aagacctaga
    10081 tggttattga tgtgaaaaaa tccaatactt attttattca gggcatgctc tttcttatct
    10141 ttgcccaaat tatggtaggc attaatattg tattttcaaa atacgtactt tcgtcaattc
    10201 ctattattta catactggcg cttcgtttta cactggctgc tgtcatttta ttactgttac
    10261 attggctaac cccagcgaaa aaattaccgg tgtattttta ttttttacag ttaaaaagaa
    10321 gagattggtt ttttattttt gctcaggcat tatctgcggg agtacttttt aatttcctta
    10381 tattatgggg cttacaatat acggatgcta acgtagctgg cattataacg agtgctttgc
    10441 ccgcaattat tgctatcatg tcgtggctaa ttcttggcga gaaaatatca ggcaaaaaag
    10501 ccatttgtgt gggttttgct actcttggat tagttgtaat tgcatgcgac aagctaggcg
    10561 acatcaacgt aagccattct tttttaggtg acgcacttgt tttaacatcg cttctgcctg
    10621 aagccatgta ttatatttta agtaaaatgc acgttaattc attacctgta tttttaattt
    10681 cttccttgct caatgggatt aatgcgattt tattgttatt ttgcttaagt tttagcacat
    10741 gggatagttt cactatccat actttggatt ggctaatttt aattattctg ggattaagtt
    10801 ccggcctttt ttatgttttt tggtatttcg gttgccagac agttgatgga gtcatggctt
    10861 cgttatcgac cgctgtaatg cctttggcaa cagtgactat tgcctggata tttcttggcg
    10921 agcaattaac gatagggcaa ataattggaa tgggcatggt tatactttca attacagtct
    10981 atgcaaaacg ttgaggtgga aaaatgacga tatcttatga agaatttgaa aaagtcgatt
    11041 tgcgttccgg taccgttgtg aaagcagaat cattcctgcg tgccaggaaa cctgcctata
    11101 aagtttgggt ggattttggt gccgatattg gcgttctcca aacctctgcg caaataacag
    11161 tgcattatac cccagaagaa ttgatagggc ggatggtcat tggatgcgtg aatttagggg
    11221 agaaaaatat tgctgggttc ctctctcaat ttctattgct tggtttttcc gatgagaagg
    11281 gtgctatatg tctggcttcc gttgatcccc atgttcctaa tggtaaaaaa atgcactgat
    11341 tcctatctga aatattaaca catcatcatt atcaactatg gatttgtttt tagctccatt
    11401 gattacattc attgatatta aaaataataa tatcagatat aaataaaatt cgttttcaaa
    11461 ataacagtgc aatatgtaaa ataattaaac aataaagggt aattattcta accttggaga
    11521 cgaaaatgag cataacagtt aaatccttag attttgatca atgtatctca aacaggaaat
    11581 ataaagagtc attacaaaca aatgatggga ggaaagtttg ggatgctaac agtttatttg
    11641 acgccaataa ggaaattctg ggtaaaaata ataatggcga tcccatccat gtttttattg
    11701 gttctaacag gcaaaacctc aaagcggatt taatcaattt aaatgcagga gcagctactt
    11761 tatttatacc agtagctcaa gagttgtgca atatcatggg ggcaactttt catccattac
    11821 ttgttcctga tttaatctgt gagaatgcca caattggaga tacttttcac agtgctctgc
    11881 aagtgattaa aggcttgaat gacttgaatt cattaaacag taagtctctg gcagaactgg
    11941 taaaatcggc gctcagtggc caactaaatt cactccattg cataagcgat gagagtaaat
    12001 ttttaatgct gtattcacag atacagtata tggctcaaca atatcctgat gagaagatta
    12061 attttgagtt ttacgatgac aaggaagata tattaaagcc actgtatgac atcttctcta
    12121 aaaatcctga tttaatacct gctaatgtga ctttgaatat taaacgttat ttgaatggca
    12181 atcttatgga gactgacttt agtccaattt tgggcctggg gtctcaacaa gagaattatc
    12241 agaatattgt aaaatggatt cataaacaat caagcagtca cctaaagtca gggaattgtt
    12301 gccaagtctt agagatggat aatgaaaaga ttgcaagata ctgccgattt ggaaaagatg
    12361 agacaaggct aaaactatta gattctcttg agaatcttgc aaaacatcaa gtgggtcaaa
    12421 aagatcaaaa aatggacggc tttatcaagg agtcttatga aaaaatgggt agctcaaaag
    12481 atatggattc tataacgttg caacaatcat ttgaggaaat tagtagtgca ataaaagtaa
    12541 ccgaagcgat taacaaagtc attgcaaatt atcgcaaaga ggccaaatgc ttatttagcg
    12601 ttggcatgaa tgccaaagcg gacagaatag aaaaggcatt actcaacgtt cccgtggaag
    12661 atcgaggtaa aattttttct aatgacaaag tttctccgga attaattgcg ataagagcgg
    12721 ctttggcttc acaccgctat tttgggaagc gggggaatgt ctattataaa gatgaagcac
    12781 gtaccgtgat tgatgaaaat aaagcggcga cgacatacaa taatttaaga aagcaatttg
    12841 cgaatttaag gacacaaagc catgctgatg cccaggttga gctggagcat tcgtctgagg
    12901 tttcaagagc tctaaaacta taaatggttc tatacatttc tattccagcc ggatttgttc
    12961 gccagttccg gtttgtttta ttactaccaa aagtttaggg tctgtttaca tttcatttca
    13021 cggctgcaaa tgcagctaat tggcgccatt atgagcttga attcgttaaa tagggctcgc
    13081 tattcgcctc attcaagctc ataatggcac ctaattatcc acattttcgc tatgcgaaac
    13141 gaaatgtgaa cagaccccat taaactgtca tttttaagca atgttcgttg tgataataag
    13201 gtaatatttg atagaagaga tctgagagat ttatgtatcg attgccaatt ttttatttga
    13261 gtgagatagg tttaagctgt gagtccatac aaaatgaaat ggttcaaaac gaaggtttag
    13321 ctcatttaaa agttttagag aaatttgacc cggaatttgg gtgaccacat gtcgatacat
    13381 tacggggttc aaaaatgaca ataagaatga gctcgtattt aatgcagaca aaggggtata
    13441 gtgttagcaa ttgtttttga tactccgcga aaggtgattc cctccgtttg tagagagcaa
    13501 tcaggtggca aggaaacacg cctttataac gatttaattt aaaggaagat ttgatatttg
    13561 atgaccattt atcaaattta atacagtgag gtgtttcatg gtgaaaccac ttaatggcac
    13621 aatgaaatat ctctcaaagg ataggttgga tattactcaa aaccaagcca atcaactcat
    13681 ttctgcagaa atgactttgg gtgatttacg tctggcacca aaaaactcaa gaagaccttg
    13741 gtgaaatatt acatataaaa taagatggaa tctctcgttt ggaaaaatgt tctgataggt
    13801 taatttcaac tttgagtaaa tatagttcag ttatgggagg atcattaaaa ttaatggctg
    13861 aatttcctgg caatggaaag taatacaagt cttcccattg cattctagcg actaaaggcg
    13921 ctgttaggag cttttgataa acttgcgagt aatgatgact agtgccactg caccactgac
    13981 ttgtgctatg gggataatca ttggtgctgt gagtgggaat aatagagcga taccacacgc
    14041 agcaagagct gttgttttgg caccaaaaac aatcaatttt tctgatgttt ctcctccctt
    14101 actcgctttt acatctgcga tgtgttgatt tttaatgtta tttttttgct gtttttcgat
    14161 caaatccttt tgggtggaac taaaagtgat gtcatgatta gatataatgg acatgatact
    14221 ctccaaaagc gaaccaatta attatattta atcataaaga ttaaaaaatg gacaattaaa
    14281 tataatcata tgtgatatat actatttgta atatcttttt gttttcaata agttatataa
    14341 gataaatgct gaatatatat taaaaatatg tggttttaat ggcgagcttg ggcgatgtta
    14401 atcagtaccc caatggaatc attttgttct cagatcatag gaaaaagctt agccgcattt
    14461 tccttgctca ttgcgcaaag taatccaaat caaaaagcat tcttatttat tcagttgtag
    14521 attaacagca ccctaaatga ctatagccaa taaaagtaat tgctcgaaat aagtattggt
    14581 tgccttcttt ctatttccgt ttaggtagtg ccacaaataa cgttccagta tgatttaact
    14641 gatttgcttc cacacctgcc tgatttccct cacctttata gacgtcaaca tgagccccta
    14701 taatggcgcc gcctgcatcc tgagccacac accatgaagt agcttttttg caatgataga
    14761 taaaactcat tccaacagga ataaccctgt gatcggttgc acatgacaca tggggggtta
    14821 gagaaatatt ttctgaacca taagggccac catcttcttt gacaaaaaat acatagcttt
    14881 gatctcgatt ccgtaaattg tctgctttat caggggtaag attgttcaga tattgccgaa
    14941 tgagcttttc tgatgcagcg ctttgcctga tttttttaga tacgttgcag cgtaacttgg
    15001 cttcatcatg gcatttcgga tctttagcgc aacgttccat tgtgtcaaga ttgccaccac
    15061 aggttggatc ttgagcgcat tggactatgc gaccaagcat cgtgcgtggc ctgccgttgg
    15121 caccgtcgta attgatgtga aacaatttgc catcaaggag taatgaacca gagccttgta
    15181 gcattaaaaa agcagagtca tttgggtctt taagatagcc aattatatat tttggattta
    15241 aagccccatg ctcaatttcc tctcgatctg gtgctacaga gtaggtgcca tcggcatttt
    15301 tcagacaaaa tccacgcatc actttgctaa gcggatccac tccgcaaaga ggagttttca
    15361 aattatattt tttactttct aacgcaacgc taacaacccc ggggttgcta tagagaggat
    15421 acagaaatgc tccaccacgt ttggagcggg cctcaacagc ggctggagcg taataggcag
    15481 taaattgaaa ctcacctttt ttaaatcctg cgtggttttc cggccagcca tcgcttttat
    15541 accagtcaaa ctcggttttg atactcgata aaaatttttt aaaatcgcca tgggcggctt
    15601 ttgccagagt cagcattttt ttgttggtat ttaagcacca ttcctggcgt ttgaattgat
    15661 ggccagcgat tgttactgtc tggaattcac catagctatt attgcagttt tcaatctgat
    15721 tatttaatgc ctggataact tcatccatat tacccacagg gttatctaac gggagttctg
    15781 atgctgaaat tttatgaaat ttaaaacgta acaccccggg ttctttggct gccattttct
    15841 gtcttgaggt gatatccacg agccttctaa tatccaggca gcgctggatt ggatcagcag
    15901 ggagggctgc ttgagtgatg aagctaaaag tcattagtgc aattattaga atggattgaa
    15961 acattggtat cctttagctt agctatggtt gaggcattgc ccgtccattg atttcaatta
    16021 gtaacatgaa ttatataggc tattggccaa ctcatcaaag agatgaattt acataaattc
    16081 tgccacccta taactacctt atcattatac agaaatagtg tcgaatccca attgtcccta
    16141 tttaaaaagc gtttaaaacc aaaagcttac atacatcagt ggatgctctt aacttatctg
    16201 cgattgaagc acttcttctt aagcttgaaa ctttattgtc cagaaaagac tttttaagtt
    16261 attgtcaaat atggcgtata gcaccggtta gcctcagtgt ttggttgaac ctggcgattt
    16321 attaatcata gtgttcaaca taaaaataaa cccataattt tttattgggt ttatttaacg
    16381 ggataacacg gataaatttt tcataaaaat tatatgattt tcttttcata agattcattt
    16441 ttcatttaaa atggaaaaaa taatgacaat ttggtttata aatgactttg atattgaaaa
    16501 tgaaaaaatt taaattgatg gcgaaagagc aatgttttgc ctttataaaa aacgctgtaa
    16561 atgttggtat ggatgaggtt atctatagta acggtacgaa taagactttt attaccgatg
    16621 gattggctac ctgtgctgct gttgctatct acggcagtga tagcagttgt tctgctgcct
    16681 ttactcatat gtctagtgaa gcttcggaca aggatgataa acgaaaagaa acgatattaa
    16741 atgagatgtt ggaatatgtt ttaaaaagca attcattatc cgatgttaaa ttaattattt
    16801 cgccttccag gattcaagaa aaacatttga tttcctttat tctggaatgg gtaaagcaaa
    16861 aaaaaattgc ttactccctg ctagaaacag ggggtgattc agcagtattc aatatagata
    16921 tggacagtcg tgtattaatg ctttcaactt cactgcaatt aaagcaggat gtatccgata
    16981 aaacatggca tggtcatggt attatcattg gccagagtga gggtattcca gtgattgaga
    17041 agcataaaaa acactttgta ttctttgcag aggatatttc ctctcaaatt accaaatttt
    17101 cagaccctcc taaaacagct ggtttagcag ccgcagtaag tgcttagaac aagcacgagc
    17161 tggataaaat gaaatgaatt tcgtatctta gtcaaaattg aaatatttcc tcaatgctga
    17221 gtagatatgt taatttcata atagattgtt ctttatgctc aatttgcagg tggtgtatga
    17281 tgagaagaca ttttattatt atcgtgcttg gtatatttca gttcggttgt atccctttgg
    17341 cagaacatca taataagatt tcacctgcta cggcgcatga agagtaccat gaggttaatt
    17401 tttccaattt atatgaaaaa aatcgctctt ttccagcatt agaaaccggc atttatgcta
    17461 ttttggatgg aatcttgatt tcctcaagtc agaatacgca tcttggtaaa aaacaatcga
    17521 agcttctgga ggaatatgta cgtcaaaatc aattattctg tcaaaaaggt tttgatcagg
    17581 actggatgaa tcaattgaag cagaatttgg acaggttaga aaaaaccgaa aataatcgga
    17641 ttgcatctgc tgtaaataaa aaaatgcaaa cgctgtttgc tgaacaacag tgcaacgcgt
    17701 tgtaaccaag gctcgtcatt ttatcaggca gcctggttca aatcacatgt gaactcaatc
    17761 tttcgaacta ttgatcacag tatttttatg cagcatatca tgtcatgaaa ggggcatgcc
    17821 attttaattc catgctatct agtccaggat aaagcaggaa agacatacaa gcagatatgg
    17881 taatatataa ttcatattga cttatttttg attaaaatat acaataatca acttcattaa
    17941 tttgtagttc tattttattt agtgagatgt gctaatggaa ttcgaagatt ggtttctgga
    18001 acaaataaac aatggtaaaa aatgtatcct gataggggaa aaccattatt cctatgcaag
    18061 cgaggactgt ctcgattgca ttttaaaatt gcaaaagcaa ggtaaaataa acaagaaaat
    18121 tgtatttttt gatgaaaatt tatctgcgtt agataatttg gaatcttaca ctgttgaatc
    18181 tttgagcaaa ttaaatacag ttgaaccttc tttaattgcg ctaattaaaa ataatgttga
    18241 ggtttatggg ttggaagata acgtcagcaa tccatttata aatcttaaat ccatgaaaaa
    18301 agtgactgca cgtataaaag atttgggttt gcaaattggg aatcgaatat ttaatgattt
    18361 tgatcattgt ttgtactggg cctaccatct ctatggaaaa tcattggtta gagtgcaacg
    18421 cggtaacgaa ggattttgta atcaaatttt gcataagatg gatgaggata ctatagtcat
    18481 tgccagagta ggggccaaac atatccctac ggtacgaaaa gatggagtag ttattgaaga
    18541 ggggatgctg caacgattag gcaaagacat ggcttctgcc tgttttatta cacacttccc
    18601 taaaaatagg acttattcag aacaaaaaac gtatcagcca gagtccgatg cttttggaag
    18661 ttatgattct atagaatgtt gcatggttca aaagaagatg attcatcagc cgggtttgac
    18721 tctatgggct gaacaagata aacaaagaaa agataccaat gactacaaag atactaaaaa
    18781 acaccgttgc acaataatgt aagtttaaat tcccttctac aagctaactg gggtcaggac
    18841 agcctgtaat tgaaaaattg gaaaaatttt gcgaatatac atttcatcgc ataacttaat
    18901 catgataggg atgtctgatg agtttactaa aggaatttaa agaatttgcc atgcgaggca
    18961 atgtgatgga tttggctgtt gctgttgtaa tgggtgttgc ttttaataaa attgtcactg
    19021 ctttggtcga tggaattatc atgccctgcg ttggtttgtt attgggaggc gtcaatattg
    19081 caggattgag ttttactgta ggagacgctc aaatcaagtg gggaaatttt ttacaaaacg
    19141 tcattgattt tattatcgtt gcttttgcta tttttatact catcaaatta atcaatctgc
    19201 tgcagagaaa aaaagcaaat gaaccagaac cagtcacttt ggaaattcaa ttacttactg
    19261 aaatacgtga tctgcttgca agaaatagca gtaaaatctg aaattggctg gcaaaaataa
    19321 acctctattt atttgcagtc agatatcccc attcaaaatg atttgttaaa cagcttatgt
    19381 tttatttcgg tgagatgtat acagaattct ggtgtttcaa tcgaccactt aaatctcaca
    19441 aagccatata ttgatttatt gttatttaaa ctatactagc agacaaggct aatttttact
    19501 taatgatcgc aaatgaaact tataattaat aaaaaaaata gggcggagtc gatggcgttg
    19561 gatgttgaag caacaacgac agtgaatgag ttgcttttaa ttctttatac tcatcgcagt
    19621 aaaaggtacg agcctttgtc ttttagtgag tatcaaaaat ttatttccgt ttaccaatgt
    19681 ttattgtatg gggatcaatt tataaataaa ttacttccgg gaaccaattt agctgattat
    19741 caacttgaag atccatgtca tttgacctgg gaggagttta ccgagaaaca gtgtctttta
    19801 gcggatgcag agaccagtag aatccttctt tggcaagcta ataagaaagc aagtaaacct
    19861 gaaattgaaa aagatcttat gtctgttgca agcagtgatg ctatgaatcc tgatatgtaa
    19921 caagaataaa acgtcctgta taacatagtg agtgttcgtt ttgtaattca ttacttgagt
    19981 catgcaatga aaaaattaaa tacatccaat cagttaacca gtgaaaggtc ttgagtcccc
    20041 tatggcctca atataaggtg ttgcccattt aataaattct taagctttta ttgttaaaat
    20101 tttgctcact aaaaggtcat aaaaaatgat ctttttgctg cggcctatct tctccacaag
    20161 gatatacgga ggactagtgg taacacttag ctttaattaa gtaaacgggt tcgtcgctga
    20221 ggcaaagaaa atgagcgatt tactctcgga tttggtgatt gagagtagtt tattaacttt
    20281 aattcagcga ggtgttactt aacaaatcgt cgtttccttt ctttttgtct ctaaattgaa
    20341 atatctatgg cctctgtcag tctttattag gcagagggtt tgttaatgta taaggaattc
    20401 ataatgaaaa ataatgattt taataatcag ttgtttcatt gagatagagc aaggaattgt
    20461 cattatgaaa tcaatgaaaa tcatctcggt ggacaattaa ttgattccag tccaggtgat
    20521 gagaaaaatt catgaattag taatgataca agctgcagcc agggtttttt atgaaaacaa
    20581 aatatgaaat aagtcaagat aaaactgagt ttttggctaa agaacaaagt agttcttatc
    20641 cagggtatca agtcagcgtt ctggatctcg aaaaaatggt taagcattat caggaaaaat
    20701 acggtattcg cttgattatt aatggcacga cacccaaata tcaggctctg attaaagatc
    20761 gccaggttaa ttttgaacaa caaaaacagc aattcctgga acttaaatac gccaagtttt
    20821 tacaaatatt ttttcagcca cctaatctga atggcgcaaa ctctcctttt tctatcaata
    20881 aatatatggg tgcatttata ggtttttacg aagaaatata caataaagtt cttccatttc
    20941 tggatgcgaa aggaaaagtt atttctggtt tatccctgga agaattaaga caactcaatg
    21001 aggcctgcca agagctgtca tgcaaaggaa tacttgatgc aacaattgat gaatttatag
    21061 aacgaaattc tgattatatg gggttaactg ccagggaaag cgcaagtgaa atgaaagata
    21121 tctgtgatga actgcaagaa ggagaggttt tgggttattt ctttactggt caacgtacca
    21181 gtgggagatg tcatttcgat ctctatatct gtttacctgg taaggccata agaccgatat
    21241 tttataacac agcgcttatc agatatcatg atcttggagg gatgtttcat ttgaattttc
    21301 cttttgtcga aggcaatttt tttacacctg atttgttaaa attatattct gcaatggact
    21361 tgcaacagct aatccctcaa gctgacagga cttcatgtgg tactttgaca atgatgtatg
    21421 ccaaggagtt attaaaagat gatgcgagag ggctgaaaga gtttacgttg agttttactt
    21481 attataatga gaaaggagaa aaggaatatt tttttctccc atcaccacaa gtattacgat
    21541 attctcaaat aagtttatat aatgaagcgc tgaaagccat tttatctcat gaaaatgatg
    21601 gacaggctgg tttggtcagg aaaggcgcta aaaaatatat gtttcacact atcgaaaaaa
    21661 ttctcatcca atcttttaag attgcacttg agaaagaaga tgctgatgtt ctggaggaaa
    21721 atcaaaaaat ttgggatata ttacccagct tccaggaaaa atggcaggag gcttataagg
    21781 aaatggttgc catacgcgat gtgatgcacc aaggggtaaa taaatatttg ctttattcca
    21841 cccatcggat gagccatatt gcaagtgatg agtccataaa caatgaagcg gatgctgata
    21901 gattaattct tcgttaaaaa atattgcaga atgaaactat tcgtatttta tttctataaa
    21961 gcatgacatt aattgagtct ggctcaatta atgtctgcaa gaaaatcagc tctttactga
    22021 tatgatgcaa gagagatgat tcatattaag ttcaaattct tggcatttat cgagaaaatt
    22081 ttttgcttcg tttggtttga taaatcggaa tcttgaataa atcgagtcgc atcaagcaag
    22141 gattccattg ggtaatcgga ggcaaacaat accctgtcct cccccatgac ctctagaaca
    22201 taacgtaagg cgggaacgaa gaaacgtccg ctagtggtta tgtaaacatt ttgacgtaat
    22261 acctctgagg gaatacgttg aatagaaggt cgataagctt ttaagtctgg tatacggtat
    22321 aaatgatcga gacgctccat taaaaacggc atggtttcac ccaagtgccc cagcataatt
    22381 tttaatttgg gaaacgcatc gaatacacct ccaagaatca ttcgcatcag acataaagcc
    22441 gtgtcaaatt caaaccctaa agcacttccc cccaaggcaa aaccatattt accgaattcg
    22501 cgcatgatgg ggacattcgg atgcaggtag ataggaatgt ttaaaccttc tgcggcttcc
    22561 agcaatggcc aataggtttt atcgtccaga taattatcct cgccaaaatt ggagtgtgta
    22621 agccaaccta caaaacctaa ttgagatatc gctctttcta attcttttac accttctttg
    22681 acgtcataag gcgatataga agcaaaacct ttaaatcgtt caggatgtct ttgaatggca
    22741 gagtaaagct ggtcattgac ttctcttgct aaagcagtgc ttttgatttc atctccaggg
    22801 caggagtcaa tcccattgat agtagtcaaa cttaatacct ggattgtcac ccctgcttgg
    22861 tccataattg ccagtcgttg ctcgtttaaa cttaataatt cttccattaa tgcacttgtg
    22921 tgaaataagg aaacatcagg tgtaaaacac attgtataag ctccattgag ctctggaacc
    22981 aatttgggaa cttcctggcg ttgagtcaga taatcaatac aagctttggt gatgaagtgc
    23041 gtttcaaaat cgatgatcat gaacgtaatc cttttagacg tgagaaaaac gattgaaaat
    23101 tacatgctta agtatagccg gaagattaga taccgccaca tggctataaa agttctgatg
    23161 gcaaattgcc ggaaaacgtt ttaaaaaaaa gaaatgctac cagcggaaag aagagatcat
    23221 tttgatgagc gagaaacata atctggtttg caccagatta taaatcaaca gggctattta
    23281 aatattataa aaaaacatca ctataaatat ttgttgaagg aacacccgca agaaaaattc
    23341 gtgttttcaa tttattggtt gtttcctttg gaccacagag atacactttt gtagtgttgg
    23401 gagagtgcag gtgagttagc acccgttttt caattgagcc ttctggataa agcccttgac
    23461 tttgcagcac acagggatca tagcggaagt tgctgaacaa taaagacaac atttccagtt
    23521 cttccttgta atagatatcc tcatcagtaa ctccgccatg caccagggta attgttcctt
    23581 catgcttttg tgttaatgcg cttctgatga tacctatcag cggagcgagg ccagtccctg
    23641 tccctgccag caatatatca aaagccagat tatgtggatt atgataataa cattgaccaa
    23701 aagggcctcg gatggcgatg aatgtattaa tggcggcgcg ttgcaaaagc cattgtccca
    23761 tcttgccttg cgggtaaatt tttatgtgca gttcaatgta tccatcatca gcagggatat
    23821 tggctatgga ataattgcgc attgttcctt caggattaat gagcgacagg tattgtcctg
    23881 gtagccatgg ggcaggatgt tccaccttca gtttgacttg aataacattg tgatttaagc
    23941 gttgcaggct cattatttga gcttgtgtct cgcattcggc attgtcagga gacgcgacat
    24001 aaagcgaagt gcttggtttg gcaagacaag ccaggaaata cccttgagat ttgagtgttt
    24061 cgggtaatcc ttcttgccag tctggattaa tctccccgtc tttggcttta atcaggcatg
    24121 actggcaaat gcctgcctgg caggagttag ggtattctac cccgtggcgc aaaaaacatt
    24181 gcaagaggct ttcatccgga gttaatgcaa aagattgatt gttaaatcgt acggtactca
    24241 tgatgagcgc cccaatacat cgccacgaac actattggct attgcagcta ctttctgaat
    24301 gtcctcttca ttagcaccca attccttaag ggtttcccct aaatgctcga ttacaatatc
    24361 cacatgtgag tcatttaagc ccctggcaag tagatgctga tgtccttccc gcatactttt
    24421 tccagtgtat tgatttggtc cgccaaacac catggttaaa aatccttttt gcttaaggat
    24481 ttgttgctcc atatccacgt catcaaaaaa ataattcact ctgtcatcca tgagcatttt
    24541 gcggtagaaa atatccacag ccatgttgac ggcattttgg ccgcctaatc gctcaaacaa
    24601 tgattcagtc atgatatacc ccttaaagat gtatttgaaa tacatcttat aaaaagaaat
    24661 aatagacgtc aacaaaccag aaggattttt ttatgcaatt gactcaattt actgattatt
    24721 ccttacgcgc cttgatctat attgccctaa agaaaggttc atgtaccatt aaggacatta
    24781 cagacgccta taccatatca aacaatcatc tgatcaaaat cattcataat ttatcgaaga
    24841 tgggtttaat taaaacaata cgagggaaac atggcggtat tttgatggca gcctcacctg
    24901 aaaccattaa tttaggtcaa ttgattatgg agcttgagcc caattttgat ttggttccct
    24961 gttttaataa agaaaaggca aactgctgca ttgccccggt atgtaaactc aagagcgttt
    25021 tgtatgaagc gcaacgtgct ttcctgcaga ttttagagca gtatactctg gctgatgttt
    25081 tacataatcc aagtgaattg tctgctttat tgaggatcag ttagcaatcc agggtaatat
    25141 tttatgaaag tgatctttat ataactttct gggcaagcaa cagagcaaat aatccttgca
    25201 ctggtcttcc caattcatcc tgtttatgaa gatgtcctac tgattctttg tattcgagga
    25261 tagaccagcc gctgtcctgg taaaaatgat acagctcttg ttgttttggt aaaaacgtga
    25321 atgagcttgg gagtgaataa agttcagatt ggacgggaaa aaccagaaaa tgatagccat
    25381 ttttcttcgt tgcctcctgc atctcaatca ataaagaggg gatacgtgag gcatttaaaa
    25441 attggagagt cacggtagag cagacaaaat cgtaatgatc tggtgcaagg gattgaggtt
    25501 gatttaaatc ctgcgacaaa atattaagtg gactcaggtt ttctttttga gcgattattt
    25561 gaatattttc cagtgccgtt tgattgatat caattccagt caatctatgg ccaagttggg
    25621 ccaaatacaa cagatttcta ccggaaccac aaccgacatc cagagtggtt agtgagccta
    25681 aatgcgacaa gtaggtatga tacacataca ataagtcacg gtgcacctca gttaagccgt
    25741 attttttgtt aaagtagcga tgaggtttgc aataaaaact aagctgagca tcgaacgatg
    25801 cgctgatcgg aatgatttta tgccaggagg ccggaggaat agatagttgc ctattatttt
    25861 tatcaagacg aatacgcgac agttcctggc ccaagccatt ctggaaaata aaatcaattt
    25921 caccttcgtt tagggaaagt ttgccccatg ttccttcttt agtgctgtgc ttttcaagaa
    25981 aaaatttaag ttttccatgg tcatcaatgg atagttgttt gtagcagatt aaattatcgt
    26041 attctcttgg catggttttt tatcctaagg tatcgataaa taacataata gttttttagc
    26101 ctattgtcga atgcatcaat tttatatagg acttacgaaa aatcccaatc tctttgttaa
    26161 aaaatgtttt taattcggtc atttagtgta tctaaacttc ctcattaaaa gcattttttg
    26221 cctcgacctt ggggtttttc gtaagtccta ttttagagtc gttaaatgag gcaccacaga
    26281 aacgtttaaa ataaaagaga ctagttatgt aagaggtctg gtcatgcttc ttgtaaagta
    26341 atgaattact ttaattagcg atgcagccat tgcgccagag cataaatacc tgattttaaa
    26401 tcgtcttcat tgcctccaaa agacaagcgg acgtaaccct ctttaccaaa ggcgattccc
    26461 ggtaccatcg caacatttgc cttatccaaa gcttgtttac aaaactcctc ggaattttga
    26521 tttttgcagc caatatcctt tagggaaaga aaaacataaa gtgaggaaat tggaggtgtt
    26581 atggtcaatc ccagatagtc atgcaaggtg cggaggaggc aatttcttct tttttccatg
    26641 ttttgctgaa cccagatatt gacttgattt acttcttgca gcgcagccac agcggcccat
    26701 tggctaaggg tggttactcc gctggtgctt tgactgacca gggcgcttaa aggcttgatg
    26761 aatatttccg gggcaaaaat aaaacccact cgccacccgg tcatggaaaa gttttttgaa
    26821 cagctttgaa tgatgatgac acgctcccga aactggggta aagaaccaca ggatacgtaa
    26881 tgatgcccat cataggttag ttcgctgtaa acttcatcag agatcactaa cagattatgt
    26941 tcctcggcta cttgtagcaa gtgaatcagc tcggactggg tatacagagc tccggtggga
    27001 ttgctggcat tattcagaat gagtatttta ctttttgctg aacaagcctt tttcagagcc
    27061 tgtggcgtta gtttccattc ttcggcttct gtcgtttcaa ctggcacggg ggtgccggca
    27121 aacaggctaa ccatggctgg ataggaaacc cagtagggag aaggaataat gacttcgtcg
    27181 ttaggctgca gtaataattg catgagtaaa taaatgccaa gttttccacc atttacaacc
    27241 aggcattgct cattttggaa tgagcaatca taggatctat acatccattc acagcccata
    27301 tgacgtaatt caggaattcc cattattgga ggataaaggg tatgtccttg ttccatagct
    27361 tgtgtgacgg catttattat ggagggatgg gggggtaatt tgggttctcc agcactgaga
    27421 ttatgaattt tgattcctgc gttaattttt tgttgagcca gagaattgat ggcaactgtt
    27481 gcggaatctt tgggcaagtt ctttttttca caatggttca ttatttttgg accttttttg
    27541 agtttgagat aattatcttg aataattgat tcacgaaatc ctgctgcaag tgatattgcc
    27601 tggataaata gtcataatat tcaaacaatt ttttttcgcg caggcggtct attatttttt
    27661 ttccttcttt agcttttaag gcgccgattt gcttagacaa ttcttggcgt tgagccaatt
    27721 tttcaataat gtatgcatca gtttgttcaa ttttttttct tagttcttca atcgtactca
    27781 tgatgaactc cttaccagat agggacctta tcactctatt ttttaactaa tataaaagcc
    27841 gttttattct gatagctgtc ctgaatattt ctcgctctca tttgcaaacc ataaagtggt
    27901 gcacaacgtg ccgacgcaat gacggcggta tggggtggta atattccttc tgccagatct
    27961 cttgctgctt tggctgtatc gatccattct atttttttcg catctttaaa tttgtgtcgg
    28021 aggaattttt tgcactgggc aagaccttga ggatgcgaaa caatatgggt gattttatga
    28081 tgttgagtat tcggtttaac cagcaggcat tggttaatgt catgccaaat ctcatcgatg
    28141 ggagtaaatg aatatttgcc catagcctga aatgctggtt ttaccaatcc tcctaaaagg
    28201 ttaacaacgg gtaagatgcc caaatctata gtttcattct caatagcatt taaaacgcct
    28261 tccatatcca gcaaatgaat gagatggaca ttaatatctt tctctttggc ataaagtagt
    28321 gctgcttcct cagaaaatga tccgatatct ccagaaacac cgaagcgaat cgcttctttt
    28381 tcttcaacga gattcttcat aacgcttccc tctctgccag ttccaacagg aacgattctg
    28441 tcttctccca actgaggcaa ggatcagtaa cggataaccc gcttaaatca atgttatcgg
    28501 gattgataga atctaatttt tgattacctt ctttgatgaa actttctacc atgaatccct
    28561 tgaccagttg ttttaaatca ggtctgtttt tgatgctttc cagaatatta aatgcaatct
    28621 ctggttgtag ctgatgattt tttttcccat tcaccagaca gttgtcatgg ctaacatcaa
    28681 taatgacaga agggttcact atctgatgca tgcccatgta gtacatgact tcctccagat
    28741 gagaaacaga gtaattggga gcttggtttg aaccacgcag aacgagatga gcatgatgat
    28801 taccatgggt ttgcacttca tagccatcga agacagcgac atgaggatgc tgtgatgcaa
    28861 ccaggctgtt tactccgaca gcgagtgaac catgtgtagg gttttttaat cctacggcac
    28921 agtcaagggc ggaggcaaaa attcgatgct cttgatcctc tgaactacgg gcgcctattg
    28981 caacccagga cagaagttca agaaatcctt tggcattatg ggtgaaaaga gcctcatcag
    29041 cgatagggag ccccatttga atgactttta tcatcatgtc cctggtatat tttatgcctg
    29101 cctcaatatc aggagttgaa aataaatcgg gttgattcac cggtccagtc cagcctttgg
    29161 tggttcttgg cttttgaatg taaacgcgca tgattaattt taaagcgtgt tttactttgc
    29221 tgttgagttg taccaaacgc tcagcgtatt ccaataccgc ttttttaggc catgctgaac
    29281 aaggaccaac tatcatcaaa agacgtttat ctcgcccttc caaaatagct ttgacctctt
    29341 gtcggtcttg agcaatttgt agataggcgg ggtgggatag tggcaattcc cggataattt
    29401 tttctattgg tggcagtttt tttaagatgg aataactcat cgtatcctcc tcaagccaga
    29461 aagcgcttta gatggatgat gaatttgaca ttgatggttc atcgttcaca ctcctctttt
    29521 gtacccaacc cccatattag actggggatc ttgggtatca aaaaccccca gactcagctg
    29581 ggggttggag tgttagtatt gcctacctac cacccagctc agggctgaaa aagctaaaat
    29641 aaaaaaagca agcaaaagcc tgtacaaggc ttgctttttt atttaactga tttggtgtag
    29701 tagtgtagaa catgaataat cacaaaaaat ttaatttgta tattatgtat acaggactat
    29761 ggtaaacctg tcaacacttg ttagcgatag ttaattttat agccaatttt cccctgggaa
    29821 ttaacccaat gatttacata taaaaatcac tttggcatta atagaactca ctgatcggaa
    29881 aatgagatca aagtattgcg cgaatcattt tgatcgtata cattgcattt tatatcaaat
    29941 aaaaactaag ggattaatgt tgtgtcaata tgttgggatt taatcataaa tcataatacc
    30001 ctgctgcaaa ccaatttcac tggtcaccgg gttcaaatga atgactccga atccagccct
    30061 aaaggaattc ttttcgtatc aaataaacta tttatcgatt tactcactta tatcactgag
    30121 tatcagtcca ttaccagaaa agaactcatg aatctttcaa ataatgaaat caaaccaagg
    30181 aggaaaagca ataagagact attaatgtgg tgagggtttt gaatttacaa aaaggagtca
    30241 atatgtttaa tgtggataat agtggtaaag gcaattgctt gtattatgct tacagtattt
    30301 cattaatgta ttatttgcgg gcaaaaaata atgtcaggat tactgaagat atttttaaca
    30361 aattaggatt aaaggaagaa gatagggccc gtttacgaaa actattatcc aaagaccctg
    30421 acagggcgtt tacccgcgat gaaatcaaaa caatcataga acccattctt ggcagggcaa
    30481 cgagagattt ggcagcagaa cataccaaag tcgaattcaa atcttcacct catgatacgc
    30541 ctttattttc atctctccat tatgctgtgg agtttggttt taaacgctca ctacaaatca
    30601 atgaatccga gttgactctg ttaatagaca atgatttctc gaatcctgat tatactgagg
    30661 ccgagatata caaggtaagt ggtttgttgg atgctcttca ggaatatatt ttaacaagaa
    30721 caccttccgt catagaagaa ttcaatcgcc aatgggaaaa taaaaaacag gaactcaaag
    30781 agcaaagctt aactgaaaaa gagatccaga ttcatcaggc gactatttta gataatatcc
    30841 taagaaaaga aacgatagat tttttattag ctgataatga aaaacatctt gatgaataca
    30901 gagagcatct tcgaagagag ttcgtatggg gtagtgagga aacactgatg gttttacatc
    30961 gcgccataca aggtgaacgc atggtacgta accatgaggg aagaattgag cctgtctatg
    31021 atcatgaaat tattttgcat gttcatagaa atggagcaag tccgccctat caggcaggta
    31081 gtccggaaat gatattaaat aatgaaggca atgttcattg gacttctatc attcctgatg
    31141 cgatatttac ctcaaaacta acagacaaag aagaaaaact actcgaaatg ctggagcgga
    31201 tgcagtcaga gtatggcagc aaagagcttg gggtagaaaa atactctttg ataagtgatt
    31261 ggcttagtga tttgatgaag cagatagagc ttattaaaac cagcccaact gctgtgacaa
    31321 aacaaaaaga aattgaattg ttttttcagc ttctgtcgaa agcaacacct aaattagcat
    31381 cggaacctgc cttgaggatg tcgcttggta ctcttttttc caattttctc gaatgcattc
    31441 cagcccttat ggttgaaaat aaactcagca caggcttagc taaatttgga ttgtatagtc
    31501 ctgtaatatc tcagaaaaaa gaagatttgt ccttgatcaa atcatctgag gagaaagcta
    31561 attctgaaca gcctcaaaag ggcgtattaa gtcaattctc gaaacatact ctgcttaagg
    31621 tatctgagga tgtaagccca aaaccatacc ctacagaact aaacccgtat gttcgaaaaa
    31681 acaagcagtc tggtccaaga gtagcgagag cactcaccca aatgtaccaa aaagggcttg
    31741 aaggtgatga aagatatacg ccagaaaagt gcaaagacat tgcgcagagg tatatagagt
    31801 attcgaaatc tcataaacgc aatttcattc ttgatgatgt catcctcttt gtgcaggaaa
    31861 tggataaaat cattaagcaa caagaatcaa ttagtgaaaa ctcctctaag cgtgctgcaa
    31921 tgtaagggtg ctgtttttgc ccgaagtgtg ggaaaaacaa c
//
LOCUS       DACTJE010000018        31448 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.018, whole genome shotgun sequence.
ACCESSION   DACTJE010000018 DACTJE010000000
VERSION     DACTJE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 31448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31448
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.018"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            301..1563
                     /gene="rho"
                     /locus_tag="I9760_000631"
     CDS             301..1563
                     /gene="rho"
                     /locus_tag="I9760_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAT5042421.1"
                     /translation="MNLSELKQLPIADLVNIAQEMGVENTSRMRKQDIIFAILKAHAL
                     KGEDIHGDGVLEVLTDGFGFLRSADGSYLAGPDDIYVSPSQIRRFGLRSGDTISGKIR
                     PPKDSERYFALLKVDQINYDTPDSAKRKILFENLTPLFATERLVMEQGNGSTEDLTAR
                     VVDLCAPFGRGQRGLIVSPPKAGKTLMLQNIARSIEKNYPECYLIVLLIDERPEEVTE
                     MQRSVKGEVVASTFDEPANRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRLYGAARNIEEGGSLTIIATALVDTGSKMDEVIYEE
                     FKGTGNMEIHLSRNIAERRVFPAININRSGTRREDLLLSPEELQRTWILRKILQSMDE
                     CDAIEFLLERMKNHKTNAEFFDAMKRQE"
     gene            1708..3174
                     /gene="ubiD"
                     /locus_tag="I9760_000632"
     CDS             1708..3174
                     /gene="ubiD"
                     /locus_tag="I9760_000632"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAT5042422.1"
                     /translation="MKYSDLRDFIAQLESRELLKRIDYPVSPHLEMTLVSDKVLRSGG
                     PALLFTNTPNYNMPVLTNLFGTVERVALGMGEESIVALREIGKLLAALKEPDPPKGFK
                     DAFSKLPLLKQALNMAPKYVSGAECQTHVWEKDEVDLTLLPIQTCWPGDVAPLITWGL
                     VTTRGPHQSRENMGIYRQQLLSKNKLIMRWLSHRGGALDYQAWQQEYPKERFPVAVTL
                     GADPATILAAVTPVPDTLSEYAFAGLLRGQRTRLTRCIGNDLHVPASAEIVLEGYLEP
                     GNEAPEGPYGDHTGYYNEVQSFPVFTVERITHRDKPIYHSTYTGRPPDEPAILGVALN
                     EVFIPLLQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYPGHAKRIMMAVWSFLRQFMYT
                     KFVIVCDDDVDARNWQDVIWAMTTRMDPSRDTVMVENTPIDYLDFASPVSGLGSKMGM
                     DATSKWPGETQREWGKPITMDEDVLNRVNGYWSLLGLK"
     gene            3174..3887
                     /locus_tag="I9760_000633"
     CDS             3174..3887
                     /locus_tag="I9760_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAT5042423.1"
                     /translation="MTDKIIQAQVEDISPLTDSIVRLVLMPDEYVDYQAGQYLQILFA
                     NDAFSYSIANAPLGSHRYELHIRHSQDNDYHQSLLAHIKQYGSLRLRLPLGECSIDQL
                     HPQKPILFIAGGTGFAPIKAMIEQLLADSDSRPFELFWGARSQSDLYMDEKVRSWRSH
                     ASHFQYFSLLSNKSEDTLASRVLARHANILRDWQIVISGPFDMVYSTRDTLVGYGISP
                     TQMFSDAFSFESKTDDAKN"
     gene            3906..4100
                     /locus_tag="I9760_000634"
     CDS             3906..4100
                     /locus_tag="I9760_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042424.1"
                     /translation="METLYQILGLIGAGLIIFILYRFIKGSPGQFSKENMSKSFMTMG
                     VLGLILIGFIALLVLMLRNT"
     gene            complement(4138..4449)
                     /gene="hspQ"
                     /locus_tag="I9760_000635"
     CDS             complement(4138..4449)
                     /gene="hspQ"
                     /locus_tag="I9760_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAT5042425.1"
                     /translation="MNKIAKYNIGDLVIHKRIRYRAIVIDVDPIFQASGKYNPRATQH
                     EFATRNPWYRLLVDDSSQITYVEECQLLPDTSQEPINNPNIDDYLIKNKGSYQKTIPS
                     H"
     gene            complement(4511..5236)
                     /gene="tatC"
                     /locus_tag="I9760_000636"
     CDS             complement(4511..5236)
                     /gene="tatC"
                     /locus_tag="I9760_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HAT5042426.1"
                     /translation="MSFMLIHLIELRQRAIYTLLWFGALALLFFFISDQLFHLLVRPL
                     LRSLPNQEGLIATQITSSVFTPLKLAVDTALLLTAPFALLQLWRFMRPGLYKNEQEQI
                     RGTIILSLLLFVFGALFCFYLVLPYMFQFFAQALPEGVRLMPDMAYAIDFITRMLLLF
                     GFSFQVPLICLVLVKTHFTTVETLIKIRPYVIVGAFIIGMLLTPPDVFSQVALALPLC
                     LLYEAGIILARIYGKSPMPTLIQ"
     gene            complement(5342..5743)
                     /locus_tag="I9760_000637"
     CDS             complement(5342..5743)
                     /locus_tag="I9760_000637"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAT5042427.1"
                     /translation="MAYRKMLKSKIHRACVTQADLDYEGSITISPELLKVANILPYEA
                     VNVWNITAGTRFETYAITGEKGSTDICVNGAAAHLVTPGDLVIIASFTQILEEDCAAH
                     EPTVVFVDQFNRLKEIRPERIGVKSRIPYPA"
     gene            6079..6849
                     /gene="rsmA"
                     /locus_tag="I9760_000638"
     CDS             6079..6849
                     /gene="rsmA"
                     /locus_tag="I9760_000638"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAT5042428.1"
                     /translation="MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGA
                     LTQPLLQKLNRLTAIEIDTDLQNYLTRLPVSQGKLNLIPADALTVDFCQFGPHLRVVG
                     NLPYNISTPLLIYLLKFITCIDDMHFMLQKEVVERIAATHGTKAYGRLSVMLQYHCEV
                     EYLFDVPPEAFEPKPKVDSAIVRLTPYRVSPFESVNTEKLENIVAKAFAMRRKTLTNN
                     LKGIISLSQLNDLGIDGGKRPEQISVAEYVQLAKFISN"
     gene            6886..8223
                     /locus_tag="I9760_000639"
     CDS             6886..8223
                     /locus_tag="I9760_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HAT5042429.1"
                     /translation="MFHRQRKSSLSAQSKSLKDLIRMVTAEQFLSEEKRQVLLQKMRA
                     FSGLEASRYESLCSVLVENLVNYCQNLPETANSYYSQPGGLVDHALNRTEAALSLFKE
                     FTVQEQPDLLSEEQKLWQYALYSAAILQGLGKLFDEFRINLFDSNGQLLKQWNPLLES
                     LIQTGSYYDYQFEKEADAELRRRLNLLLARTLMPASGFAWIASNPEVLAVWLALLNED
                     YRSAGTLGAILIRADAIAIQRYFLAFMARSGARRSGRYGRAVTFTGGVPESITEKEQA
                     IGVEFIQWLIKSLDEGRIMVNKAPLFMVPGGMLMCPEMFQLFVREHPEYKNWQAIQNG
                     FLSLGLHGRDAGGNVTSRFEQAHTQQMYTGVVFAGYGVALPNSVQVHHLNTGKIESMS
                     ALELIHKSQYTSQFTQQSSAIAIAPLQRLAANGQWQNIEQDMSQLTFGAKRGA"
     gene            8216..9046
                     /locus_tag="I9760_000640"
     CDS             8216..9046
                     /locus_tag="I9760_000640"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HAT5042430.1"
                     /translation="MPDYAIGDVQGCYDPLQRLLELIDFNEKEDCLWFVGDLVNRGPD
                     SLAVLRFIYSLPVKPKITLGNHDLHLLGLLFGGQPWKGHDDTLEEVMLADDGEELGHW
                     LRKQSLLCRSSELNIVMCHAGIAPLWDLSKAVGLANELEAVLSGDSYHEFFAQMYGNK
                     PDIWSDDLVGLDRLRVITNYFTRMRYCDAHGRLDLGYKGTLSKAPNHLYPWFEVPCRK
                     EIEMDIVFGHWAALMGRSSHPRIHAIDTGCLWGGQLTALRLQDRQRFSVPGYGVSRFE
                     "
     gene            9090..10718
                     /locus_tag="I9760_000641"
     CDS             9090..10718
                     /locus_tag="I9760_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAT5042431.1"
                     /translation="MKFLNKSTALLPVIILSGLISFHAASAKTSSNADNSLYSWTENP
                     SAEAQRVEQAEERLKAAIANPEYMLKNWIELPTSPESKKKTRHQLTIREAILLALRYN
                     PNIQNAELDRIIQRYQLRLANNEFELQYALGASGVVQKSTFEGIGSSTNHSFLASPEL
                     DLKTKIGTKASVSIDNNVSTNNSYNPVLNLSITQPLLRGFGKSANEAKLLDAIDKEWL
                     NKLSLQQSVMDQITQVITAYRALILSGNNLENQRLQLEEARKSYRINEKKIAAGQLEP
                     TGNIQQSYQIESLSLMVEQGENDFKLASQELLQMIGLDPDLHISVPSDVTIKEIIVPD
                     LQESIAIALSHNTQYLAQKMALRADERAYTVAKNQQLWQLDLAGTVQGGAVNDVTGTS
                     SGLSSIYNGNNITESARVTLTIPLNDIGRRSQLINAKVQLEKDKINLIAAKRALITSV
                     TNTINNIQSLAKRYELAQKQVKLAEQSYALEKKKQQAGISSALDVNNTQNQLIQAQSG
                     LIGAKIAYLNQLSALQRILGTTLDYWQIKLRYGG"
     gene            10715..11860
                     /locus_tag="I9760_000642"
     CDS             10715..11860
                     /locus_tag="I9760_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HAT5042432.1"
                     /translation="MNNYLKIFSIVLLILMLVSCGSSDKPQTSLLKTYEVKPQPLHKT
                     LHFTGTVQPIRESSLTSPMEAVVETMHFHYGQMVKKGEIVLTLNSNELQKQYNDTLTD
                     YLKAKDSYSIAKAKFVGTEELWNAGLISKNNYLSEKSGLDTARVTLMQATRKLSEMLE
                     KMDDKNTQNISNLSLADFDKVRKILTSNHNLIHLKAPSDGIMLYPPKSGEDKNNRVTV
                     GSTIKSGQVIALIGDLKGISVEIDVPEIDIDKIRPGMQATISGVAFGRHQLKGKLVAV
                     NAQASNTSTGGLPSFTAVVEVNSLTPEQQSWIKVGMSASIELNVESNNQLLIPISAVK
                     REKGSSIVQVQLAKGKVEKRMITTGAAQADSVVVESGLKSGDVVVYD"
     gene            11853..12542
                     /locus_tag="I9760_000643"
     CDS             11853..12542
                     /locus_tag="I9760_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5042433.1"
                     /translation="MIDRPLIQLSDLVKTYHLEGISTTVLKAVSLTVNEGDLLAIVGA
                     SGSGKSTLMNIIGLLDKPDAGTYTLNNRNVASLSDDEAADLRNQNIGFVFQQFNLLPR
                     FTAMQNVALPLTYRGVNPTLIKEKVEQALDKVGMRQYIRHRPTQLSGGQQQRVAIARA
                     LVTDPQVILADEPTGALDSRTGSEVMNLFLALHQEGRTIIMVTHDEHVAAQCKRRITL
                     ADGAIIAESGQ"
     gene            12539..13732
                     /locus_tag="I9760_000644"
     CDS             12539..13732
                     /locus_tag="I9760_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAT5042434.1"
                     /translation="MNFLNHCQQALVNLTASKLRSLLAVLGILVGTAAVVALISCGQL
                     ATEKALEQFKALGTDLLAVSVYQKVPSKSHSESDSLTIDQWQQIAERIPYVLRIAPYS
                     TAYQTLSFNGKVMQGAVIGADESLADIIHVTLARGHFVSFLDSFEHFCVIGDSLAQQI
                     KEITLDDPIGKQLRIGQSLYTIIGVAERWKENGFFNEDINQAVIIPLAGMSLVSKDAK
                     INNAVLLLKPDSPIDEVINEIKEIINSQAPKLSVFPRSAKQIIASMENQGRIFTLLLA
                     VIGGISLLVGGIGVMNVMLVSVSERKKEIGIRKAVGAKNREIQALFLVESVMLSLLGG
                     VLGVILGLIFTRIVAYFSDWTFTIYLLPPIAGFLVSAATGIFFGFYPARRASKLEPMV
                     SLRSE"
     gene            complement(13805..14131)
                     /locus_tag="I9760_000645"
     CDS             complement(13805..14131)
                     /locus_tag="I9760_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAT5042435.1"
                     /translation="MNEQLNHLYGLPSHAIEALKCVFKEYSQIDNAILYGSRAKGTYH
                     QGSDIDLCLTGNLLGITELLAIENKIDDLLLPWKVDISLKHTIDNPDLLEHIERAGIL
                     FYTKES"
     gene            complement(14124..14543)
                     /locus_tag="I9760_000646"
     CDS             complement(14124..14543)
                     /locus_tag="I9760_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAT5042436.1"
                     /translation="MTNIDVRWQQRLNNYARALQQLSLAVNLAQTRPLSDLEKQGLIQ
                     AFEFTHELAWNVMKDYFFFQGNSAITGSRDATRESFNKGLIKEGEIWMEMIKSRNQTS
                     HTYNQSVADEIVKNIINFYHTSFQAFLEKMQGLKEHE"
     gene            complement(14777..15955)
                     /locus_tag="I9760_000647"
     CDS             complement(14777..15955)
                     /locus_tag="I9760_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAT5042437.1"
                     /translation="MHIKNIFTWNLMGAVILSMPLFAGEQVKLKSIDSLIDKYIEKYK
                     NEELVTGVGATFLSGTEIISTAHGFENNKRKKRLTNNAYFQIGSITKSFTAALILKLE
                     AEGKLNINQTIGDWLPEYDDWKGITIKQLLNMTSTIPGYATIPTLADSITDNPKKQWT
                     SKELVDLAYGYKHPTSGYNYSDTNYLILGMIIEKVTHDSFHNQLQKKILKPLQLKETY
                     YITDSYTPYFLKKMPESYYYGRTVENLKHGQKSTNDNLSMAGPAGAIVATTSDVAKWI
                     KALFSSDFLLPKQQMELKSLISMKTGTPISHVDSEEPNGFGLGVVQGYKPQLGKYWYY
                     EGDTVGFRVLYIYHPCNEKIVVIALNSSPFVDEAAGDHSGDLLLELYKNAFGISQKCE
                     "
     gene            16485..17447
                     /locus_tag="I9760_000648"
     CDS             16485..17447
                     /locus_tag="I9760_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HAT5042438.1"
                     /translation="MKKWGLAIVALMLFNQITYASQSWNQWVAGVREEALQQGIPPSL
                     FDEAFAGIHEPSRKIKGFLRSQPEHRLTFMKYRNTRADNYRIVIGRKEYKKNKEILEE
                     IAQQYGVNPCFIVSFWGMETSYGSYMGNFPVINALATLAYDSNRKDFFRKELFLALHI
                     LKGGHVSLADFKGEWAGASGQPQFLPSSWVKYAVDYDGDGRKDIWKSKPDVFASIANY
                     MKQNGWQAGEPWAIQVKLPAKFDMKLEGKGIVKPVSEWNALGVRTESGEPLPYQNLQA
                     SIVQPYGGPTFLAFPNYKMILRYNNSIYYAGAIGYMADKICQGQ"
     gene            17751..18797
                     /locus_tag="I9760_000649"
     CDS             17751..18797
                     /locus_tag="I9760_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT5042439.1"
                     /translation="MNQKSFQWSVVGAGPAGIAAVGKLLDYGIAPSEILWIDPHFKVG
                     DFGQLWRHVSSNTTVKLFSSFLHAANSFSYKKAPSTFRLNNLDPDNTCLLSDVAEPLQ
                     WVTSVLVNQVIAEETVIHSLSLSGGCWTLCSDTQQYRAKNVILATGAVPSSLNYPGVN
                     VVPFEIAIDKQRLAHVVDTNETYGVFGSSHSAIMILRSLIELGVKKVINFYRSPCRYA
                     INMGNWILFDNTGLKGDTAAWARKHIDGVLPANLVRYYPSELNIARHLPECDKVIYAV
                     GFERRKNMTIDNYEHVTPNPHVGIIGPGLFGLGIAYPELSVDPFGTIEHQVGLWKFMV
                     YLCKVLPVWFKYPA"
     gene            18907..19410
                     /locus_tag="I9760_000650"
     CDS             18907..19410
                     /locus_tag="I9760_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042440.1"
                     /translation="MIKILIYLGLFLSSNLLMAKPLSQLDSANLLPCFNIEQAERIGK
                     QINKLLQHEFCEENINPKKFASISHNILPKIMTETFLGVTPPENWQQLSDDIIKNCIA
                     NKNLCKKAARKELEECIKPRIPLILIQFGPWLAQNCPQLNKSLIEQWPNKQATLKKII
                     NENKSAE"
     gene            19615..19951
                     /locus_tag="I9760_000651"
                     /pseudo
     CDS             19615..19951
                     /locus_tag="I9760_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19954..20244
                     /locus_tag="I9760_000652"
     CDS             19954..20244
                     /locus_tag="I9760_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT5042441.1"
                     /translation="MDKKLFERLYESMTQMNEIIDGERVPSREFVVDAVHVKEIRKKT
                     GLTQEKFCHLIDVSIGTLRNWEQGRREPTGPAKALLRAINKDPEHVLAALAG"
     gene            complement(20533..20724)
                     /locus_tag="I9760_000653"
     CDS             complement(20533..20724)
                     /locus_tag="I9760_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042442.1"
                     /translation="MRNKDEKAKEVATDSRCAEANRLTQLITGAILADNCFFPSGVYS
                     IKPMVSSPQPPQEKTTPSM"
     gene            21092..22534
                     /locus_tag="I9760_000654"
     CDS             21092..22534
                     /locus_tag="I9760_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT5042443.1"
                     /translation="MKISTHELIAVVSQFNPWWRGESIQDLPTWHRAAYQELELWLIK
                     PPVPRAIFLSGARQIGKTTLLLQAIDKLLKEGVKPGNILYVTFDHPLIKMSDIDAVLE
                     AWREREPKTGDLEYIFIDEAQFMRSWGTWIKHQVDFSKSRRIFFTGSATPIIEADQES
                     GVGRWHTIRLTTLSFYEYLQIKKIDLPQLPTVKTLRELFEWPQQEFYKVKELAVHYVG
                     HFHEYLLRGGFPQTAQVENITQAQRLLREDIIDKVLKRDMTALFGVRRILELEQTFLY
                     LCMHDGGLLDMTDLCSNLEVKRPTIQNYIFLLEATNLIYRLPPFGYGKDILRAKYKIY
                     LADAAIAPAVLLKDKSILDDPESLGIATETAVFKHLFARYYSQNVRFSYWRGTKDHEV
                     DVVAQIGDQIIPFEVKYRSQHTNAKALKGLLELCKKKSIAHGYVVTKSLDDFGIMKAL
                     PEIKTNIMQIPAPLLCYWMGESELRQTFGV"
     gene            complement(22696..24183)
                     /locus_tag="I9760_000655"
     CDS             complement(22696..24183)
                     /locus_tag="I9760_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2912 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042444.1"
                     /translation="MSYSKIDVLERKWSSLEAIVKSYTDPVEYHLSEIKKLMTAEFVV
                     DFSSHAIYLHTEYVHRFTQQLINLRNTSPNDEKLLYIHQLWTDLLNCRNQNPLKIKHH
                     KKLDGECTFNFSLKMMEGKPYGFKEVQILDPNNTAELNKAMGEIKRIDSLAHGANFGD
                     LPFIGDIVLSSMLHYKETICFIAKDKNDEVIGYCWGMMLRDIPAGEKNKVNVFWVMNL
                     AKHPDFYDPNIKVGEALRQHLANTLSKNPDCHFLGYEHVLNHNFHLAIVDGGVPKKEE
                     TISIGQNQYQAKSLVKYEPLKMQYDRKHIIKTNNHQYPYPKVDVIKSNFFTALWYAAP
                     TAFDFILGGILIYGRAYYQQWTHSMLGQPVTHRIEKPISQEQQICDLNILKRIILSDV
                     WGQQGTTLFFDACIPNTIQKLQQLVKEDQIEFPSLQQCVAKSGWALCRSKLTAYFYFA
                     ITQSQYPSAAINLLIQNDMTPKKWIELISEERQISAQQSSERASI"
     gene            24501..24602
                     /locus_tag="I9760_000656"
     CDS             24501..24602
                     /locus_tag="I9760_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010280437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 domain-containing protein"
                     /protein_id="HAT5042445.1"
                     /translation="MLKFSRIFPNREIVSTLSKQLSWSHFVMIWVLM"
     gene            24689..25960
                     /locus_tag="I9760_000657"
     CDS             24689..25960
                     /locus_tag="I9760_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S10 family peptidase"
                     /protein_id="HAT5042446.1"
                     /translation="MSIFRSVLITLLFISSAMAATPDQVTYLPGWGPIKNNQYAGYFP
                     VNPKAGLFYWFVESNNPSMDAPIVLWLNGGPGAASLYGFFMENGPYQVDKNGKLTARK
                     DSWTKAANYLVIDQPAGVGYSYGSSKSYGSEGEAIDQLQGALQLIFKKHPELYGKPLF
                     LAGESYAGKYLPQLAIRLLKDKNMNLKGLLLGDPWINPRLQQKANIDYAYYHGLIDNK
                     ARKRVRVLYENCVKEIDKQSPSTSKANKTCEQIQEFIKQESGGLNLANIATGVEPEDT
                     NMVNYLNQKVVREALHIPVTVSEFKTFSTAAAKKLEIGEQDSVADLYPQLLAAGIRIL
                     IYNGLEDGKDSNFLSTELLLASLDWHGKNAFAKAPTCIWRTNNEVSGYAKGAVGLTQV
                     KIRGAGHLAPIDQPARVFDLFKHFINDKPLC"
     gene            26817..28751
                     /locus_tag="I9760_000658"
     CDS             26817..28751
                     /locus_tag="I9760_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HAT5042447.1"
                     /translation="MHVLDPLTLEEIQESCDILKREKELSDSYRFAWVMTYEPPQQEV
                     LHSSDTDFDRCAFLSVFNKKTNETFEAVVNLNSKKVVEWKQIVFEAPPYGKPPILIED
                     FQKCEQIVKADPTWRAAMEKRGLDDEQIDNIQVDAFSAGYFDEEEEREQRIVKGISFY
                     RDNLKDNGYARPIEGVVAIVDLGNEKVLRVIDDGRNVPIPKEKINYDSDSYPEKRKDL
                     KPLDIIQAEGPSFKVNGWEVNWQNWSFHVGFTPREGLVLHQIKYLDEGKERPIIYRAS
                     VTDMIVPYADPSVSHYWKSAFDAGEYGLGKLANELKLGCDCLGNIYYFDVPAADDFGN
                     SRLIKNAICMHEEDAGTLWKHYETRNSTFEVRRARELVVSFFTTIGNYDYGFYWRFGQ
                     DGSLKLEIKLTGIVQTAAIFPEAQYEWGGKLTPELAAPTHQHFFNARLHMMVDGEKNS
                     FSEHEFYPIAMGGKNPYGNAFGSSQKTFEHEGEAARSACAQTQRTWKIFNPNSVNGIG
                     TAPAYKLELPETPLLLADEESYIYKRGGFASKQVWVTQYDPQEKYAAGDYPNQSSGGC
                     GLPAYIKQNRKIDNENLVLWVTLGSTHFPRPEDFPVMPTSIISAKLHPFGFFKRNPAM
                     DLPQGKVQEKGDTCCSSLTY"
     gene            complement(28799..28987)
                     /locus_tag="I9760_000659"
     CDS             complement(28799..28987)
                     /locus_tag="I9760_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HAT5042448.1"
                     /translation="MADIINLNKKRKAKVRLEKEIKASENRIKFGRTKKEKQQEKQDN
                     ERSERHLDGHKLDKKEEN"
     gene            29648..30517
                     /locus_tag="I9760_000660"
     CDS             29648..30517
                     /locus_tag="I9760_000660"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HAT5042449.1"
                     /translation="MSNYLDKTASLTPATKRIVCNKATEYPHTGSYNQVATHGTYLCR
                     RCGLALFRGVSQFSSGCGWPSFDDEIANAVARKPDADGQRTEILCARCDAHLGHVFTG
                     EYMTYKNLRHCVNSASLDFVADNEVLDTEEAILAGGCFWGVEYYLKRIPGVLKVESGY
                     TGGHVSEPSYEQVCSGTSGHYEAVRVVYNSAKTNYHDIVKRFFEIHDPTQANGQGPDL
                     GHQYLSAVFYFNQEQLQEAELLIQLLKKRGYQVVTQLLQAQPFWPAEDYHQDYYFKHH
                     KIPYCHKPVDRFG"
     gene            complement(30541..>31448)
                     /locus_tag="I9760_000661"
     CDS             complement(30541..>31448)
                     /locus_tag="I9760_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042450.1"
                     /translation="FSAHDLEVAFSLKKKYRESKESGYPFTGFVCDYERRDKLYDFIY
                     SSCHSPKLFENNFKVQIAYLDREKSGVRHWSLIELQYSIEKGNNTLKILVLDSLGAQL
                     SYNTAQILANEVGFQDLLDENEDLSIKIYIPDVKLQHSRAGCATFTMDCASMLSTQDK
                     YENIYDFMDKQAEVSDKVKIAKLDADFPVHCASLPPRILRGAQSRAIITDDKVLNSTP
                     LINSKEETFKHSLLKHCSFFEDQPQTPENIKRNERIMKKALKWRNTIDLKMKTSSAEE
                     FENTCAIDDLEEYANSCLPSKSVQL"
BASE COUNT         9404 a         5908 c         6771 g         9365 t
ORIGIN      
        1 tgagatgaaa atttatttac agggctttta cttttttatg gggcttgtgt taaaattaac
       61 ctacttaaag ggattttttc ttaaagattt aaaagttaaa atcaaaaaag tcaaaataac
      121 tttttatttt tagtttaaaa atgttggatc ttgttgtaaa gctgtgatag cctgtagcta
      181 tatgtgacat gctttaaaac aagtgagcca cataggcagc ggattcctta gctgctattc
      241 cctaacataa ttttttcttc ggatattcaa ttcatttaaa caacaaatca agtgagaagt
      301 atgaatctta gtgaacttaa gcaattacct attgccgatc tcgttaatat cgcacaagag
      361 atgggtgtgg aaaatacctc ccgtatgcgc aagcaagaca tcattttcgc catccttaaa
      421 gcccatgctt taaaagggga agacatccat ggcgatggtg ttttggaagt tttgactgat
      481 ggttttggtt tcttgcgttc cgctgatggc tcctatctgg caggccctga tgacatttac
      541 gtctctccca gtcaaatcag acgtttcggt cttcgttcgg gggatactat ttctggtaaa
      601 atccgccccc ccaaagacag cgaacgctat tttgctttat taaaagttga tcaaattaac
      661 tacgatacac cggatagcgc aaagcgtaag attttatttg aaaacttaac tcccttgttt
      721 gctaccgagc gtctggttat ggagcaaggt aatggcagca cagaagatct cacggcaaga
      781 gtagttgatt tatgcgctcc tttcggccgc ggacagcgtg gtttgatcgt gtcacctccg
      841 aaagcaggta agacattaat gttgcaaaac attgctcgct ctatagaaaa aaattatcct
      901 gaatgttatc tcattgtttt gctgattgat gaacgtcctg aagaagtaac ggaaatgcag
      961 cgttctgtca aaggtgaagt tgttgcgagt acttttgacg agcccgccaa tcgccatgtt
     1021 caggttgccg aaatggtaat agaaaaagca aaacgtctgg ttgagcataa acgtgatgtg
     1081 gttattctgc tggattcgat cactcgcttg gccagagctt acaatacggt tgtaccggca
     1141 tcaggtaaag tactgactgg tggtgttgat gcgaatgccc tgcaaaggcc caaaagattg
     1201 tatggtgcgg cacgtaacat agaagagggc ggcagtttaa ccattattgc aacggctctg
     1261 gtagacacgg gttccaaaat ggacgaagtg atttacgaag agttcaaagg tacaggtaac
     1321 atggaaatcc acttgagtcg taatattgcc gagagaaggg ttttccctgc gattaacatt
     1381 aatcgctctg gtactcgccg tgaagatctc ctgttgagcc ccgaagaatt acagcgcacc
     1441 tggatattgc gtaaaatcct gcaatccatg gatgaatgcg atgcgattga attcttattg
     1501 gaacgcatga agaatcataa gacaaatgcc gaattctttg atgcaatgaa gcgtcaggaa
     1561 taatttaaat gataattaac cccgtaatta gcggagcata attcggggtt caaattcgcc
     1621 ttattttcag gtttataatt catgctcata gcctgtatta cgctgtgctg gtacgggttt
     1681 acagtaagct tctttttgag ataattaatg aagtattcag atctgagaga tttcatagcc
     1741 caacttgaat cacgtgaatt attaaaacgt attgattatc ctgtatcacc tcatcttgag
     1801 atgaccctag tcagcgataa agtgttgcgc tcaggagggc cagcccttct gtttaccaat
     1861 acccccaatt acaacatgcc tgtactgacc aatctttttg gtacggtaga gcgcgtggct
     1921 ttgggaatgg gtgaggaatc aatagtggct ttgagggaga ttggaaaatt attggctgct
     1981 ttaaaggagc ccgatcctcc caaaggcttc aaagacgctt ttagcaagtt gcccttattg
     2041 aaacaagcgc tgaatatggc acccaaatat gtcagtggag ccgagtgcca gactcatgtg
     2101 tgggaaaagg atgaagtgga tttaacctta ttgcccatcc aaacgtgttg gcccggagat
     2161 gttgctcctc taattacctg gggtttggtt actactcgtg gcccacacca gtccagagaa
     2221 aacatgggca tctatcgcca gcaactatta agtaaaaaca aattgatcat gcgctggtta
     2281 tctcaccgcg gaggtgcttt ggattaccag gcctggcaac aagaatatcc caaagagcgt
     2341 ttccctgttg cggtgacttt aggcgctgat ccagccacca tactggcagc agttactccc
     2401 gttcctgata ctttgtctga atacgctttt gcgggcttgc ttagaggaca acgaactcgg
     2461 ttgactcgat gcattggcaa tgatttgcat gttccagcca gcgcagaaat tgttttggaa
     2521 ggttatctgg agccaggaaa tgaggcgccc gaagggcctt atggcgatca caccggttat
     2581 tataatgaag tccaatcttt tcctgttttt acggtagagc gtattactca tcgcgataaa
     2641 cctatttacc acagtactta taccggaaga ccgccagatg agccagccat tttgggagtt
     2701 gccttaaatg aagtgttcat tcccttgtta caaaaacaat tcccagagat tgtggatttt
     2761 tatttgccgc cagaaggatg ctcttatcgt ttggctgtag tcactataaa aaagcaatat
     2821 ccaggacatg ctaaacgtat tatgatggct gtttggtctt tcttgcgcca gtttatgtat
     2881 accaagttcg tcattgtttg tgatgatgat gtggacgcgc gcaattggca agatgtcata
     2941 tgggcaatga ccacacgcat ggatccgtcc cgcgatacag tcatggtaga aaatacaccc
     3001 attgattatc tggacttcgc ttccccagtt tcaggattgg gttccaagat gggtatggat
     3061 gctaccagta aatggccagg agaaacacaa agagaatggg gtaaaccaat tacgatggat
     3121 gaagatgtgc ttaatagagt aaatggttat tggtccttat taggattaaa ataatgacag
     3181 ataaaataat tcaagctcag gtggaggaca tttccccttt aacggacagc atcgtgcgat
     3241 tggtcttaat gcctgatgaa tacgttgatt atcaagccgg acagtatttg caaatcctgt
     3301 ttgcaaatga tgcgtttagt tattcaattg caaatgcgcc tttaggttct catcgttatg
     3361 aattgcatat tcgccatagc caggataatg attaccatca atcgttattg gctcatatca
     3421 aacaatatgg atctctgcgc ttaagattac ctttgggtga atgcagtatc gatcaattac
     3481 atccccaaaa acccatttta ttcattgccg gtgggactgg atttgcgcca attaaagcca
     3541 tgattgagca gttactggca gattcggaca gcaggccatt tgagttattt tggggagcac
     3601 gctcacaaag cgatttatac atggatgaaa aagtaaggag ttggcgctcc catgccagcc
     3661 acttccaata tttttcgcta ttatccaata aaagtgagga tactttggct tctcgagttc
     3721 ttgccaggca tgcaaacatt ctacgggact ggcaaattgt catcagcggt cctttcgata
     3781 tggtgtacag tactcgtgat accttggttg gttatggcat atcgcctacc cagatgtttt
     3841 cagatgcctt tagttttgaa tccaaaacag atgatgcaaa aaactgagtg gaatggagtg
     3901 aattaatgga aactttatat caaattttag gactcattgg cgccggattg attattttta
     3961 tattgtaccg atttattaag ggaagcccag ggcaatttag caaggaaaat atgagcaaaa
     4021 gctttatgac gatgggggtt ttaggtctga ttttaatagg ttttattgcc ttgctggtgt
     4081 tgatgttgag gaatacataa ccaactccat gccctgttgg catttccacc tcataagcta
     4141 gtgagacggg atagttttct gataactgcc cttgttttta attaaataat catcaatatt
     4201 tgggttgttg ataggttcct gactcgtatc aggaagtaac tggcattctt cgacataagt
     4261 tatttggctg ctgtcatcaa ctaacaggcg gtaccatggg tttcttgttg cgaattcatg
     4321 ctgagttgcc ctgggattgt atttccctga ggcttggaat atggggtcca catcaataac
     4381 aatcgcccgg tatctgatgc gtttatgaat aaccagatca ccgatattgt atttggctat
     4441 tttattcata aaaggctcca tttttgagca ttatgataaa tatatcggta attccgcttt
     4501 taacttgagc ttactgaatt aaagtgggca tgggggattt cccataaatc ctggctaaaa
     4561 taatccccgc ctcataaagc aaacacaaag gcaatgctaa tgcaacttga gaaaaaacat
     4621 cagggggggt gagtagcata ccaataataa atgccccaac tatcacataa ggcctgattt
     4681 ttataagtgt ctcaactgta gtgaaatggg tttttaccag tacaagacaa attagtggaa
     4741 cctgaaaaga aaaaccaaat aaaagcagca tgcgtgtaat gaaatctatg gcgtaagcca
     4801 tgtcaggcat gagtcgcact ccttccggaa gggcctgagc aaaaaactgg aacatataag
     4861 ggaggacaag ataaaagcaa aatagtgccc caaagacaaa gagcaaaaga cttaaaatga
     4921 tggtgcctct gatttgttcc tgctcgtttt tatagagtcc cgggcgcata aaacgccaaa
     4981 gttgcaataa agcaaacgga gcagtgagca acaaagcggt atccactgca agttttaaag
     5041 gggtgaaaac cgaggaagta atctgtgtgg caatcaagcc ctcttgattc ggtaaagaac
     5101 gcaataacgg ccttaccagg agatgaaaca gttggtctga tatgaaaaaa aacaatagcg
     5161 ctaaagcgcc aaaccagaga agggtataga ttgctctttg ccgtaattca attaaatgaa
     5221 ttaacataaa gctcattgga gttgaatgcc gagatcatag aataaaccat tccatttgat
     5281 ttttctaatt ttgatcattg cctgacacag tagcatatga acccattgct atgggttcaa
     5341 ttcaagccgg gtaaggaatt ctacttttaa caccgattct ttcaggtcgg atttctttca
     5401 gacgattgaa ttgatcaaca aaaacaacag tgggttcatg tgctgcacaa tcctcttcca
     5461 gaatttgcgt aaaagaggcg ataataacca aatcgccagg ggttaccaaa tgagctgccg
     5521 ctccattgac acagatatcc gttgaaccct tttcacctgt aatggcataa gtttcaaatc
     5581 gggttccggc agtgatattc cagacattaa ccgcttcata gggcaatatg tttgccactt
     5641 ttaaaagctc tggtgaaatg gtgatactcc cttcataatc cagatccgcc tgtgtgacgc
     5701 aagcacgatg aatttttgat ttaagcattt ttctataagc cattatttac ttcctaaatt
     5761 taagcctgga aaaagcaatt ggctaaatac gactgagttt atttgttaca tggctttttc
     5821 cagattgaaa taactaaaaa aaatagataa gaatatcaac cttggtcatt gtggttgaat
     5881 tttaccccat accttggagt ttacgctata taaattttta aaattgaggg agaaggcttt
     5941 gagttattgc gtattgtcgg ggtttgctgg cacttgacac taacctacgg taaaattaac
     6001 aggcttcccc aatggggtat accttgtgat gtcaacaaga ttactgaatt aattttatcc
     6061 taacataggt gatagaaagt gaggcatagc ccacgtaaac gttttggtca aaatttctta
     6121 caagacaaat atattattaa tgagattcta cgcgctatca atcctttggc ggatgataat
     6181 atgctggaga ttggccctgg tttaggcgct ttaactcagc cattattgca aaaactgaat
     6241 cgattaacag ccattgaaat cgatacggat ttacaaaact atttaacccg cttgcctgta
     6301 tctcaaggca aactgaattt aattcctgct gatgcgttaa ccgttgactt ttgtcaattc
     6361 ggcccccatt taagagtagt gggtaattta ccttataata tttctactcc attgctgatc
     6421 tatttattga aatttataac ttgtattgat gacatgcatt ttatgttaca aaaagaagtt
     6481 gtagaacgaa tagcagcaac ccacgggact aaagcctatg ggcgactaag cgtgatgttg
     6541 cagtatcatt gtgaggtaga gtatttattt gatgttcctc cagaagcgtt tgagcccaag
     6601 cccaaggtag actcagcaat agttcgatta acaccttatc gtgtttcgcc atttgagtct
     6661 gtaaacactg agaaactgga aaatattgtt gcgaaagcat ttgctatgag acgcaaaact
     6721 cttactaata atttgaaagg gattatctca ttatctcaac tcaatgattt aggtattgat
     6781 ggaggtaagc ggccagaaca aatttcggtg gcggaatatg tccaactagc gaaatttatt
     6841 tccaattagt gtaaaattat atgataagtt attgtggagg ttgtcttgtt tcatcgtcag
     6901 cgtaaaagct ctttatctgc tcagtccaag tcattaaaag atttaatccg catggtgacc
     6961 gcagagcagt ttctgagtga agagaaacgt caggtattac tccagaaaat gagagcattt
     7021 agcgggttgg aagcatctcg ctatgagagt ttatgttctg ttcttgtcga gaatctggtg
     7081 aactattgtc aaaatttgcc ggaaaccgcg aacagttatt attcccagcc cggtggtttg
     7141 gtcgatcatg ctttaaacag aacagaggct gcattgagtc tattcaagga gtttaccgtt
     7201 caagaacagc cagatttgct ttccgaggaa cagaagcttt ggcaatatgc tctttattca
     7261 gcggcaattt tacaaggcct tggcaaatta tttgatgagt ttcgcattaa tttgtttgat
     7321 agcaatggtc aattgttaaa acaatggaac cctctattag aaagcttaat ccaaactgga
     7381 agttattatg attatcagtt tgagaaagaa gcggacgcgg agttacgccg ccggctgaac
     7441 ttgttgctgg ccagaacttt gatgccggcc agtggttttg cctggattgc ctccaatccg
     7501 gaagtattgg ctgtttggtt agctttactg aatgaagatt atcgttctgc tggtactttg
     7561 ggcgctattt taattcgagc tgatgccatt gcgatacagc gctattttct ggcgtttatg
     7621 gcacgaagtg gggcaagacg aagtggccgt tatggcagag cggtaacttt taccggcggt
     7681 gtacctgaat ccataacaga aaaggaacag gcaatagggg tcgaattcat ccaatggctt
     7741 atcaagtcgt tggatgaagg tcgaattatg gtgaacaagg ccccattgtt tatggttcct
     7801 ggcggcatgt tgatgtgccc tgaaatgttt caattgtttg ttcgtgagca tcctgaatat
     7861 aaaaattggc aagccataca gaatgggttt ttgtcattag ggcttcatgg ccgggatgcg
     7921 gggggcaatg taaccagtcg ttttgaacag gctcatactc agcaaatgta taccggagtc
     7981 gtttttgcag gatatggggt tgctttaccc aactctgtcc aggtgcatca tttgaatact
     8041 ggcaaaatag aatccatgtc agcactggag ttaattcata aatcacaata caccagccaa
     8101 ttcacacagc aatcaagtgc gattgcaata gcgcctttgc aacgattggc agcaaatggc
     8161 caatggcaga atattgagca ggatatgtcc caattgacat ttggagcaaa acgcggtgcc
     8221 tgattatgca ataggggatg tacaaggttg ttatgatcct ttacaacgtt tgcttgagct
     8281 aatcgatttt aacgagaagg aagattgctt gtggtttgtt ggggacttgg taaaccgcgg
     8341 tcctgattca ttggctgtat tacggtttat ttattcctta cctgtgaaac ctaaaataac
     8401 cttgggtaat catgatttgc atttattagg cttattattc ggcgggcaac cctggaaagg
     8461 gcatgatgat actcttgaag aggtgatgct ggctgatgat ggagaggagc ttgggcattg
     8521 gttgcgcaag caatcccttc tttgcaggtc atctgagtta aacatagtca tgtgtcatgc
     8581 aggtattgct cctttatggg atttatcgaa ggccgtgggc ttggctaatg aattggaagc
     8641 ggtgctttca ggcgattcat atcacgaatt tttcgcccag atgtacggca ataagccgga
     8701 tatttggtca gatgatttgg ttggactgga taggttacga gtcattacca attattttac
     8761 ccggatgcga tattgtgatg cccatggacg attagatttg ggttataagg ggactttatc
     8821 caaagccccc aatcatctgt atccctggtt tgaagtgcca tgccgaaaag aaattgaaat
     8881 ggatatcgtt tttggtcatt gggcggcgct catggggcga agttctcatc cgcgaattca
     8941 tgcgatagat accggatgtc tttggggagg gcaattaacg gcattacgcc tgcaggacag
     9001 gcaacgcttt tcggtacccg ggtatggggt tagtcgtttc gaataacctt gagggatagg
     9061 catttttata aacacaggcg ataacaacgg tgaaattttt aaataaaagc acagcattgt
     9121 taccggtaat tattttatcg ggcctgatta gttttcatgc cgcaagtgcc aagacttctt
     9181 caaatgccga taattcactt tattcctgga cagaaaatcc ttcagctgaa gcacagagag
     9241 tggagcaagc ggaggaaaga ctaaaagcag ctattgccaa cccggaatac atgctgaaaa
     9301 attggataga gctgcctacc tcgcctgagt caaaaaagaa aacaaggcac cagttgacga
     9361 taagagaagc catactgttg gccttgcgtt ataatcctaa tatccagaat gcggaattag
     9421 accgcattat tcagcgctat caattgcgtt tggctaataa tgagtttgaa ttgcaatatg
     9481 ctcttggtgc ttcaggcgtt gtgcaaaaaa gtacttttga ggggataggc agttctacga
     9541 accattcttt tctggccagt cctgaattgg atttgaaaac taaaataggc accaaggcat
     9601 ccgtatccat cgataacaat gtttcgacca acaacagcta taatccggta ttgaatttat
     9661 ccattactca acctttgctc cgtggatttg gcaagtcagc gaatgaagca aaactgttgg
     9721 atgcgattga taaggaatgg ttaaataaat taagtttaca gcaatcggtt atggatcaga
     9781 ttacgcaggt gattacggcg tacagggctt tgattttaag tgggaacaat ttagagaatc
     9841 agcgcttgca attggaagaa gccagaaaat cctatcgtat taatgaaaaa aagattgctg
     9901 cgggacaatt agaacctacc ggaaatattc agcaatccta ccagatagaa tctttgagtt
     9961 tgatggtaga acaaggtgaa aatgatttta aattagcctc tcaggaatta ttgcaaatga
    10021 ttgggcttga tccggatttg catatttcag ttcccagtga tgtgacgata aaggaaatta
    10081 tagtgcctga cttgcaagag tccattgcca tcgcattaag tcacaacact cagtatctgg
    10141 cccagaaaat ggccttgcgt gcggatgaga gggcttatac tgttgcaaaa aatcagcaac
    10201 tttggcagct tgatttggcg ggtactgtgc aaggcggcgc agtcaatgat gtcaccggga
    10261 ccagcagtgg tctaagcagc atttacaatg ggaataatat cacggaatca gcccgggtca
    10321 ccctgaccat tccattaaat gacattggcc ggcgtagtca attaattaat gctaaagtcc
    10381 agttggaaaa agacaaaatc aatcttatcg cagccaaaag agctttaatt accagtgtaa
    10441 ccaataccat caataatatt caaagcttgg ccaagcgcta tgagctggca caaaaacaag
    10501 tcaaattggc agagcagtct tacgctttgg aaaagaaaaa acagcaggca ggaatatcga
    10561 gcgccctgga tgtgaacaat actcagaatc aattgattca ggcacagtca ggattgatag
    10621 gggcaaaaat tgcatatctc aatcagttat ccgcgttgca aaggatttta gggaccacgc
    10681 tggattactg gcagattaaa ctaaggtatg gcggatgaac aattatctga aaatattctc
    10741 tatagtgtta ttgattttaa tgctggtttc ttgtggcagt tctgataagc cgcaaacctc
    10801 tttattaaaa acgtatgaag tgaaacctca acctttacac aagaccttgc attttaccgg
    10861 gaccgtgcaa cccataaggg aaagcagcct gaccagtcca atggaagccg ttgttgaaac
    10921 catgcatttc cattatggcc agatggttaa aaagggagag attgttttaa ctttaaattc
    10981 caatgaattg caaaaacagt ataacgacac attaacggat tacttaaagg cgaaagacag
    11041 ttattccatt gccaaggcaa aatttgtagg aaccgaggag ctgtggaatg ccgggctaat
    11101 ctccaaaaac aattacttaa gtgaaaaatc cgggttggat acagcgcgag tcactttaat
    11161 gcaggccact cgcaagcttt ctgaaatgtt ggaaaaaatg gatgataaaa acactcagaa
    11221 tatatccaat ctcagtctgg ctgattttga caaagtgagg aagattctta cctcaaatca
    11281 taatttgatt catttaaagg ctcccagtga tgggatcatg ctgtaccctc ccaaatcagg
    11341 agaggataaa aacaatcgag tgaccgtagg atcgaccata aagtcagggc aagtcattgc
    11401 cttgataggc gatttgaaag gaatcagtgt ggaaattgat gtcccagaga tagacattga
    11461 taaaattcgc ccgggaatgc aggcaacaat tagtggcgtt gcatttggca ggcatcagct
    11521 gaaaggtaaa ttggtggcgg tgaatgcgca ggcatcgaat accagcacgg gaggattacc
    11581 ctcctttacc gctgtagtgg aggtcaattc cctaactcct gagcaacagt catggattaa
    11641 agtggggatg agtgcctcca tagagctgaa tgtggaatcg aataatcaat tgttgatccc
    11701 tatctcggcg gttaaaaggg aaaaaggcag cagcattgtt caggtccaat tagccaaggg
    11761 gaaagtcgag aaacgaatga ttactaccgg tgccgctcag gcggattcag tagtggttga
    11821 aagtggcctt aaatcaggag atgtcgtggt ttatgattga cagaccttta atccaacttt
    11881 ctgatttggt gaagacttat catcttgagg gtatcagtac cacggtatta aaagcagtgt
    11941 ctttgaccgt aaacgaaggt gatttgctcg ctattgtcgg tgcgtccggc tctggtaaat
    12001 ccacgttaat gaacatcatt ggcttattag ataaaccgga tgcaggaact tataccttaa
    12061 ataatagaaa tgtggccagt ttaagcgacg atgaggcggc tgatttgcgc aatcaaaata
    12121 taggttttgt ttttcagcag tttaatttgc ttcctcgttt tacagcgatg caaaatgtgg
    12181 ctttgccttt gacgtaccga ggggtcaatc ccactttgat aaaagaaaaa gtggagcaag
    12241 cactggataa agttggaatg cgccaataca tccgccacag gccaacccaa ttatctggcg
    12301 gtcagcagca gcgagtcgct atcgccagag cgttagtgac cgacccacag gttattctcg
    12361 ctgatgagcc gaccggtgcg ctggattcaa gaacaggtag cgaggtaatg aatttgtttt
    12421 tggccttaca ccaggaaggc cggactatta tcatggtgac ccatgatgag cacgtggcag
    12481 cgcaatgcaa acgccgaata accttagccg atggagcaat tatagcggag tccgggcaat
    12541 gaattttctt aatcattgtc aacaagcctt ggttaattta acagcctcca agctccgatc
    12601 gcttttggct gtgctgggaa tactggtagg gaccgccgct gtcgttgcct tgattagctg
    12661 cggacaactg gctacagaaa aggcgctgga gcaattcaag gcattgggta ccgatttgct
    12721 ggctgtttct gtttatcaaa aggtgcccag caaatcccat agtgaatccg actcgttaac
    12781 aatagatcaa tggcagcaga tagctgagcg tataccttat gtcttaagaa ttgcgcctta
    12841 cagcacggct taccaaactt taagttttaa tggcaaagtg atgcaagggg cagtgattgg
    12901 cgccgatgaa agccttgctg atatcattca tgtgaccttg gcacgggggc attttgtttc
    12961 ttttttggat tcttttgagc atttttgtgt tattggcgat agcctggcac aacaaatcaa
    13021 agaaattacc ctggatgatc ccattggcaa gcaattacgc ataggccaat ccttgtatac
    13081 cattatcggc gttgccgagc gttggaaaga aaatggtttt tttaatgagg acattaacca
    13141 ggctgtcatt attcccctgg ctggaatgag cctggtcagc aaggacgcca aaatcaataa
    13201 tgcagtattg cttctcaagc cggacagccc cattgatgag gtcattaatg aaataaagga
    13261 aatcattaat tctcaggcgc cgaagctcag tgtttttccg cgcagcgcca aacaaattat
    13321 agccagcatg gaaaatcagg gtcgtatttt taccttgctg ttggcggtca ttggtggaat
    13381 atccttgctg gttggcggca tcggtgtcat gaatgtgatg ctggtttcgg tcagcgagcg
    13441 caaaaaagaa attgggattc gcaaagcagt gggtgcgaaa aacagagaaa tccaggcctt
    13501 atttttggtg gaatctgtca tgctgtcttt gctagggggt gtgctcggag tcattttagg
    13561 tttgattttt accagaatag tcgcttattt cagtgactgg acttttacta tctacttact
    13621 ccccccaatt gccggctttc tcgtttctgc tgccactgga atatttttcg gattttatcc
    13681 ggcccgacgg gcctcgaaac tggagccgat ggtttcttta cgcagtgaat agtttattag
    13741 ggtctgttga catttctact atcttggccg aaaagccagc gaaatgtcaa cagaccctaa
    13801 agtcctacga ttcttttgta taaaacagga tacccgctcg ctcaatgtgt tcaagcaaat
    13861 cgggattgtc tatggtgtgt ttcagggata tatcaacctt ccagggtaac agtaaatcat
    13921 caattttatt ttcaatagca agtaattcag tgatccctaa taagtttccg gtaaggcaaa
    13981 gatcaatatc tgagccctga tgatatgtgc ctttggcacg agatccgtag agaatcgcat
    14041 tatcaatttg agaatactct ttaaagacac atttcaatgc ttcaatagca tggctgggca
    14101 agccatacag atggtttaat tgctcattca tgctctttca gaccttgcat tttttccagg
    14161 aatgcctgaa aagaggtgtg gtagaaatta ataatatttt tgacaatttc atcggcaacg
    14221 gattggttgt aggtatgaga ggtttggttg cgacttttaa tcatttccat ccagatttca
    14281 ccttctttaa ttaatccctt gttaaatgat tcgcgtgttg catcacgaga accagtaatt
    14341 gcagaatttc cctggaaaaa gaaataatct ttcatcacat tccaagccag ctcatgggta
    14401 aattcaaagg cttggattaa gccttgcttt tccaagtcgg ataagggtct tgtttgcgcc
    14461 aggttgacgg caagtgataa ttgttgaagc gctcttgcat aattatttaa ccgttgttgc
    14521 cagcgaacat cgatattggt cataacttac ttactccaga ttttgaggtt gtaccggtta
    14581 atttaatgaa gcgactttat cgaattataa aaactcattt tataatgaaa aacacgaggt
    14641 attccagaag cgttttttat ctaaataggt tttttgcacc ggatttacaa gtactttaag
    14701 gccataacct atatctctct tgcatgactc gcctcttttt gaaaggttgc caaaagaagt
    14761 tggattttac cctattttat tcacatttct gtgatatgcc aaaagcgttt ttgtacaatt
    14821 ccaataacaa atcaccacta tgatctcccg ctgcttcatc aacaaaaggc gaactgttta
    14881 atgcaataac cactattttt tcattacaag gatgataaat atataagact ctaaatccca
    14941 ctgtatcacc ttcataatac caatattttc ccaattgcgg cttatagcct tgaacaacac
    15001 ctaaaccaaa gccattaggc tcttctgaat caacatggga gattggagta cccgttttca
    15061 tcgaaatgag ggattttaac tccatttgct gtttcggcag taaaaaatcg gatgaaaata
    15121 aagcttttat ccatttggcg acatccgatg tagttgcgac aatagcacca gcaggtcccg
    15181 ccattgataa attgtcattg gttgactttt gaccatgttt taagttttcc actgttctac
    15241 cataatagta actttccggc atttttttta aaaaatatgg ggtataagaa tcagtaatat
    15301 aataggtctc ttttaattgt aagggcttta gaattttttt ctgtaattga ttatgaaagg
    15361 aatcgtgagt gactttttct ataatcatac ccagaattaa gtagttggta tcagagtaat
    15421 tataacctga ggtggggtgt ttatatccat atgctaaatc taccagttct ttactagtcc
    15481 actgtttttt aggattatca gtaatcgaat ctgcaagcgt tggaatcgtt gcataacctg
    15541 gaattgtact tgtcatatta agtaattgct ttatagtaat gcctttccag tcatcatatt
    15601 ctggcaacca atctccaatt gtttggttga tgtttaattt cccttccgct tcaagcttta
    15661 agattaatgc ggctgtaaac gatttggtta tactgccaat ttgaaaatat gcattatttg
    15721 taaggcgttt ttttctttta ttgttctcaa aaccatgggc tgttgatatt atttcagttc
    15781 cgcttaaaaa agtcgcaccc acacctgtaa ccagctcttc atttttatat ttctcaatgt
    15841 atttatcaat taatgagtca attgatttca atttaacttg ctcgcccgca aataagggca
    15901 tagatagaat tactgcgccc attaaattcc atgtgaatat atttttgata tgcatattta
    15961 actcctgtgg atcaaagcct cagttatact atgaacacta atttcttttt attccagttt
    16021 acttacatac aagcatatca atacctgggt tatggtattt aatctctgca tgttaaagaa
    16081 gctgtgctcc atacggcctc ctaaaatcta ttttgttatc aatggagtta tcatgttgac
    16141 aaaaaataga atggaaaaag atgcgaaaaa atcccataac aatcttcgca ggccaagact
    16201 agtttatagc cccactttct tctaatgagc gggttctaat tatagatttc catgaaagat
    16261 tactttctgt acaatactta ttgttaggtc tgactttctg gttcaagctt tgtcggtata
    16321 ttgccagaga atgacgaata gacaggccaa tttttaataa atgagctatc ttttaagaat
    16381 accagtatgt tttgagcgga ttgttttttg taacaattaa agagaaagaa acgctgggcg
    16441 aaatacatat tattaaacaa taaatataac tgaggggaag agctatgaaa aagtgggggc
    16501 tcgctattgt tgcactgatg ttatttaacc aaataaccta tgcaagtcaa agctggaatc
    16561 aatgggttgc cggggtgcgt gaggaagcct tacaacaagg gattccaccc agtttatttg
    16621 atgaagcctt tgccggcatt catgaaccaa gccggaaaat aaaaggtttt ttacgctctc
    16681 aacctgaaca caggttaacg ttcatgaaat atcgtaatac cagagcagac aattaccgaa
    16741 ttgtgattgg aagaaaagag tacaagaaaa acaaggaaat actggaagag attgcccaac
    16801 aatacggagt taatccttgc tttattgtgt cgttttgggg tatggaaacc agttacggtt
    16861 cctacatggg aaatttcccg gtaatcaatg cgctggctac tttggcctat gattcaaacc
    16921 gtaaagactt tttccgcaag gaattgtttc tcgctttaca catcctgaaa ggtgggcatg
    16981 tcagcctggc tgatttcaaa ggggagtggg ctggcgcctc tggtcaacct caatttttac
    17041 catccagctg ggtcaaatac gcggtcgact acgatgggga tggacgaaaa gatatatgga
    17101 agagcaaacc cgacgttttt gcctccatag cgaattacat gaaacaaaac ggctggcagg
    17161 ccggagagcc ctgggctatt caagtgaaac ttcccgccaa gtttgatatg aaattggaag
    17221 ggaaaggcat agtcaaacca gtcagcgaat ggaatgcttt aggtgtgcgc acggaaagcg
    17281 gtgaacccct gccctatcaa aatttgcagg ccagtatcgt acagccttat ggtggcccga
    17341 ccttcctggc gtttcccaac tacaagatga tcctacgtta taataactcc atttattatg
    17401 ccggggctat cgggtatatg gctgataaaa tttgccaggg tcaatgattt gttggagaca
    17461 taaatatcta ctgatgaatt ccactggaca ggcgttcagt ggagttctca taggccaagc
    17521 tttttaagct ttataaatca taggtaacgt gagttatgga taaaatcagt tgttatctcg
    17581 agccgctcgg gctgaggagg catttatgcc gtctcgaagc ctagcatcgg attttatgtt
    17641 tcatgccaag gcttcgagac gatgcttagg catctcctca gcccgaacgt accctgggta
    17701 tcgacgggct gttatatcct ctttagccat aactcacgtt aggtattaaa atgaatcaga
    17761 aatcctttca gtggtcagtt gttggcgcgg gacctgccgg aattgctgca gtaggtaaac
    17821 tattggatta tgggattgca ccctctgaaa tattatggat tgatcctcat tttaaagtgg
    17881 gtgattttgg gcagctctgg cggcacgttt ccagtaatac tacggtcaag ctcttctcaa
    17941 gttttctgca tgctgcaaac tcattttcct ataaaaaagc accctccaca tttcgtttaa
    18001 acaacctgga tcctgataat acctgcctgt tgagtgacgt tgcggagccc ttgcaatggg
    18061 ttaccagcgt tctggtgaat caggttattg cagaagagac tgtcattcac agcttgtctc
    18121 tttctggagg ttgctggact ttatgctctg atacacaaca ataccgagcg aaaaatgtta
    18181 ttttagcaac aggggctgtc ccctccagcc tcaactaccc tggtgttaat gtggtaccgt
    18241 ttgaaattgc aatcgataaa cagcgtcttg cccatgttgt tgatacgaat gagacttacg
    18301 gagtttttgg ctcatcccac tcggccatta tgattttacg ctctcttatc gaattaggcg
    18361 taaagaaagt gattaatttt tatcgctccc cttgccgtta tgccatcaac atgggcaatt
    18421 ggattttatt cgacaacaca ggcttgaaag gtgatacggc tgcttgggcc aggaaacaca
    18481 tcgacggtgt cttgcctgcc aatcttgtcc gttactaccc aagcgaatta aatattgcac
    18541 gccatcttcc agaatgcgat aaagtgattt atgccgttgg ttttgaaaga cgtaaaaata
    18601 tgaccattga taattatgaa cacgttactc ctaatcctca tgtgggcatt ataggaccag
    18661 gcttattcgg cttgggcata gcctatcctg aactcagtgt cgatccgttt gggactattg
    18721 agcatcaggt gggattatgg aagttcatgg tgtatttgtg taaagtgctg ccggtctggt
    18781 ttaaatatcc tgcctagagt tgggaaatga taattcaaca catagccact cttcatcggc
    18841 tggttttcta cagactcgca agactctgtt atattgacat ggttaggaaa cgccaaggat
    18901 aaaaaaatga taaagatatt aatttattta ggactgtttt taagcagtaa tttactcatg
    18961 gcgaaaccat tgtcgcaatt ggactctgct aatttgctcc cctgttttaa catagagcaa
    19021 gcagaacgca ttggaaaaca aatcaataaa ctcttacagc atgaattttg cgaggaaaac
    19081 atcaatccaa agaaatttgc ctctatcagt cacaatatcc tgcccaaaat tatgacagaa
    19141 acatttttag gagtaacccc tccagaaaac tggcagcaat taagcgacga tattataaaa
    19201 aactgcatcg caaacaagaa tctatgcaaa aaagcagctc gcaaagagtt ggaagaatgc
    19261 atcaaaccga gaattccttt gatcctgata caatttggcc cctggcttgc ccaaaattgt
    19321 ccacaattga ataagtcttt gattgaacaa tggccaaata aacaggccac tctcaaaaag
    19381 ataattaatg aaaacaaaag tgctgagtaa tcgaggcagg cttaattacg agagttatgc
    19441 cggataaaac aacatttatt tacctaattc acctaacttc atcaaatata acttaccgat
    19501 atgatctaca accaggttct taaaacgatc gttttatata catgccaaat acgtcattga
    19561 cttactttta tttaggatat atactttatt catcaaagaa aaataagaat agcaatgatt
    19621 tttattgaaa ctccaatttt tacagaagac gtgaaggaat tattatctga tgaggagtat
    19681 gctgagtttc aagagtatct tgctgataat cctttagctg gagacgtaat tcagcaaact
    19741 ggcaaactgg caaactggca aactggcaaa ctgcgaaaag ttcgatggtc atttcaaggc
    19801 aaatgggaag cgaggcggtg ttagaattat ttattattat gtaactcctg cttcacaaat
    19861 aagactctta cttatttata aaaaaggtat tcaagatgat ctgactacag atcaaaagaa
    19921 aatgttacgg caattaaatg aacggtggta atcatggata aaaaattatt tgaacgatta
    19981 tacgaaagta tgactcagat gaacgaaatt attgatggtg agcgtgttcc ttcacgtgag
    20041 ttcgtagtgg atgctgttca tgtaaaagaa attagaaaaa aaacaggcct aacccaagaa
    20101 aaattttgtc atttaattga tgtaagtatt ggaacattaa gaaattggga gcaggggaga
    20161 agagagccaa caggtcctgc aaaagctctt cttcgcgcaa taaataaaga tcctgaacat
    20221 gtattagcag cattagctgg ttgataattg atgttttaac tttctataaa aagtgcagac
    20281 agtgtcggca caattcaaag tttctctaaa ggagctttta actcaaaata tagaaaacga
    20341 gcttagcaag cttatatgac cgataaaatt tgccagggtc agtaaatagt attctgatgg
    20401 ctagataata aatgatgaaa agttcttttg atgaaatgaa taaaagaacc ttttttattg
    20461 agcgactctt aaaaaagcca tttgctttat cctgtgcttt tggcaagcag tatggtcgca
    20521 ttcaggcttt gtttacatgc taggtgtagt tttttcctga ggcggttgtg ggcttgatac
    20581 cataggtttt attgaataga cgccacttgg gaaaaaacaa ttgtcggcaa ggattgcccc
    20641 tgtaatcagc tgggtaagcc gattggcttc ggcacatctc gaatcagtgg caacctcttt
    20701 cgctttttca tctttatttc gcataacaat cctgtgaagt taatctttgc agaggacact
    20761 atgatggttt catgtcatac aaacgaagca accgggtacc gaacgaatcc ttacaggaaa
    20821 acgaagctta tggaaggcca acaaggaaca gggagtaaat tcataacgcc ccctgtttcc
    20881 atgaaaatat ttcagggggc aggacgatga atcgtattgg acaataaggc tataacgcgg
    20941 tgacggttta ataatagtct ccgtattgac tcaattacgg gcaagcaagg tctggcattg
    21001 gcccgacctt gcttaaattt ctaaaaaaca cactattatt cctaattagt tatagtatac
    21061 tttaactaaa caatcttttt agtgtttttt tatgaaaatt tctactcatg aattaattgc
    21121 agtagtatcc caatttaatc cctggtggag aggtgaatcc attcaggatt tacctacttg
    21181 gcatagagca gcttaccaag agttggaatt atggttaatt aaaccacctg tccccagagc
    21241 aatatttttg tctggagctc gacagatagg taaaacaacc cttttgttac aagctattga
    21301 taagcttttg aaagaagggg taaagccagg aaatattttg tatgtaactt ttgatcatcc
    21361 attaataaaa atgtctgata ttgatgctgt attagaggca tggagggaac gagaacctaa
    21421 aacaggtgat ttagaatata tttttattga tgaagctcaa ttcatgcgga gttggggaac
    21481 atggattaaa caccaagtcg atttttcaaa gagtagaaga atttttttta cgggatctgc
    21541 aacaccaatt atagaagcag atcaagagtc aggggtaggg cgttggcata ctattcgtct
    21601 aacaacactc tcattttatg aatatttaca aataaagaag attgatttgc cccagttacc
    21661 aactgttaag accttgcgag aattattcga gtggccacaa caagaatttt ataaggttaa
    21721 agaattagcg gttcattatg taggacattt tcatgagtat ttattacgag gtggatttcc
    21781 acaaacagct caagttgaaa atattaccca ggcacagcgc cttttaagag aagatataat
    21841 tgataaagta ctaaaaagag atatgacagc actgtttggt gttcgtcgta ttttagagtt
    21901 agagcaaacc ttcctttatc tatgcatgca tgatggggga ttgctagata tgactgatct
    21961 ttgcagtaat cttgaagtaa agagacccac aatacaaaat tatatttttt tgttggaggc
    22021 tacaaatctc atttatcgct tacctccctt tggttacggt aaagatattt tgagagcaaa
    22081 atataaaatt tatttagcag acgcagctat agctccagct gtattactga aagataaaag
    22141 tattctggat gatcctgaat ccttaggtat tgccacagaa acagctgtgt ttaaacatct
    22201 atttgctcga tattactcgc aaaatgtacg gtttagttat tggcgcggca ccaaagacca
    22261 tgaagtggat gttgtagcac agattggtga tcaaataatt ccttttgaag tcaaatatcg
    22321 ttcacaacat acgaatgcca aagcattgaa aggattgtta gaactatgta agaaaaaaag
    22381 tattgctcat ggttatgtag taactaaatc gttagatgac ttcggtatta tgaaagccct
    22441 accagaaatt aagacaaata ttatgcagat acccgcacca ttactttgtt actggatggg
    22501 agaatcagaa ttaaggcaga catttggggt ttaattagtt gataacttaa tgccgtcaaa
    22561 taatttattg aacactgacg caaaataact tatggcaaat atttatccct caaaatgttt
    22621 ctcaataagt cattgttacc ttgaaccagg gtaagcttta attttcttaa acaaaatgga
    22681 gtatatagtc ctctcttaga tggaagctct ttcactgctt tgctgagcag atatttgacg
    22741 ctcctcactg attaattcaa tccatttttt aggtgtcatg tcgttttgta tcaataagtt
    22801 gatcgctgca ctaggatatt gactttgtgt aatggcaaaa taaaagtaag cggtcaattt
    22861 gcttctgcat aaagcccaac cactttttgc gacacattgt tgcaaactag gaaattcaat
    22921 ttgatcttct ttgactaatt gttgcagctt ttgaatcgtg ttagggatgc atgcatcaaa
    22981 aaataatgtg gtcccttgct gtccccatac gtcacttaat ataattcttt ttaaaatgtt
    23041 caagtcacaa atttgttgct cttgcgaaat aggtttctca attctatgtg ttaccggttg
    23101 ccctaacatg gaatgtgtcc attgttgata ataagctcga ccgtaaatta atataccacc
    23161 caaaataaaa tcaaaggcag ttggggctgc ataccaaaga gcagtgaaga aattagattt
    23221 gatgacgtcc actttggggt agggatactg atggttattg gttttaatga tatgtttgcg
    23281 atcgtattgc atttttaaag gttcatattt aaccagggat ttagcttggt attgattctg
    23341 accaatactt atcgtttctt ccttcttcgg tactcctcca tcgactattg ctaaatgaaa
    23401 gttatgattt aaaacatgtt cataacccag aaaatgacaa tcaggatttt tagataaggt
    23461 atttgctaaa tgttgtcgca atgcctcccc gactttaata ttaggatcat aaaaatctgg
    23521 atgtttggct aaattcatta cccaaaatac attgacttta ttcttttcac cagcaggaat
    23581 atctcgtaac atcattcccc aacaataccc aataacttcg tcgtttttat ccttggcaat
    23641 aaaacaaata gtctctttat aatgtaacat tgaagatagg acgatatccc ctataaaagg
    23701 caaatcgcca aaatttgctc catgggctaa agaatcgata cgtttaattt cccccattgc
    23761 tttattaagt tcagcagtat tattgggatc taaaatctga acttctttga agccataggg
    23821 cttaccttcc atcattttta acgaaaaatt aaaggtacat tcgccatcta actttttgtg
    23881 gtgtttaatt ttaagaggat tttgatttct gcaatttaac agatctgtcc ataattgatg
    23941 aatgtaaagt aatttttcat catttggtga ggtatttcgc aaattgatta attgttgggt
    24001 gaaacgatgc acatattctg tatggagata aatagcatgg ctactgaaat caacaacaaa
    24061 ctctgccgtc attaattttt tgatttcgga taagtgatat tcaacaggat ctgtatatga
    24121 ttttactatt gcctctaatg aactccattt gcgttcaagt acatcgattt ttgaatacga
    24181 catctatatt tccttgtaat aaattttcgg agaaatctta acatggcaat ttacttggcc
    24241 aaatcctatt ttgtcaataa agattaggcc ctttttaaat ttaaaaggag ctatcttcat
    24301 atggaaaaac tataactctc gagacaggag ttttcattgt gctgtgttga cattagatag
    24361 agatcacctg cgatgggggg agggccttgg atcggatccc tattgacaca aactttctaa
    24421 aggaatactt gagaatttag agtacagata tctttcattg ccagtttggg cgtggctatg
    24481 ggaagatcaa cttatctcga atgttgaagt tctcacggat tttcccaaat cgcgaaattg
    24541 tttcaacact gtcgaaacaa ttgtcttggt cccacttcgt gatgatttgg gttctaatgt
    24601 aatgaaccgt cttacccaca atcctttgat gatggatggt ttgtttcagt gcgtgtaaaa
    24661 tattcaattt tatcaataat ggatccccat gagtattttt cgtagtgtat taataacgtt
    24721 actttttatt tcatccgcaa tggctgcaac tcctgatcag gtaacctatt tgccaggttg
    24781 gggtcccatt aagaacaatc aatatgctgg atattttcct gttaatccaa aagcgggatt
    24841 attttattgg tttgtagaat caaataatcc atctatggat gctcccattg ttttatggtt
    24901 gaacggtggc ccaggggcag caagtttata tgggtttttt atggaaaacg gtccttatca
    24961 ggtggataaa aatgggaaat taactgcccg caaagattca tggacaaaag cagctaacta
    25021 tttggtaatt gaccaaccgg caggagtcgg ttactcctat ggttcctcaa aatcttatgg
    25081 cagtgaaggg gaagctattg atcagttaca aggtgcatta cagctcattt ttaaaaaaca
    25141 cccagagctc tatgggaaac ctctattctt agctggtgaa tcttacgcag gaaaatactt
    25201 accgcagtta gctattcgtc ttctcaaaga taaaaatatg aatctaaaag ggctattatt
    25261 aggcgatcct tggattaacc cccggctaca gcaaaaagcg aatattgatt atgcctatta
    25321 tcacgggctg attgacaaca aagcaagaaa aagagtgcga gtcttatacg aaaactgtgt
    25381 taaagaaatt gataaacaat caccctccac atcaaaggca aacaagactt gtgaacaaat
    25441 acaagaattt ataaaacagg agagcggagg acttaattta gctaatattg caacgggggt
    25501 tgagccagaa gataccaaca tggtcaacta tttaaatcaa aaagtagtta gagaagcgtt
    25561 gcatattcct gtgactgtat cggaattcaa aacatttagt acggcggcgg ccaaaaaact
    25621 agaaataggt gaacaagatt ccgtggctga cttgtatcca caattgttag cggctggcat
    25681 tcgtatatta atatataatg ggctagaaga tggaaaagat tcaaattttc taagtactga
    25741 actgctgctc gcttcgctcg attggcatgg caaaaatgca tttgcaaaag caccaacttg
    25801 catatggcgc actaacaatg aagtgagtgg atatgcgaaa ggagcggttg gattaacgca
    25861 ggttaaaatt cgtggtgcag gccatctagc accaattgac cagccagcaa gagttttcga
    25921 tttattcaaa cattttataa atgataaacc cttatgctag atttggagaa agcttgctgc
    25981 attgataaat tgaattcacg aaagtaattt tttaactaag cttaaccact caaataatta
    26041 gactaaatac tatttatagt attcagcaac ctatattaca caatatttaa gtagtatcta
    26101 tcgtaccccc caaatcccct gattattgtt tagaacgctt tgcaaaaaga acaagcatcc
    26161 tttgaagtgc tggattttag tgtataacca gagtgtattc acagagttat ccacagaaaa
    26221 tggggataag tgatcaatgc aattgatgta tcaatacttg ataagtcctt gcaggacaag
    26281 gggttttgta aaaagagtat gatctgagct ggtcatgaca ttggagggta ggaatagtta
    26341 ttcacaggaa aggagatgca cttaatatgt taatcgcttt acatcgtaac aaaaaaaaaa
    26401 ataaaaaaac agtcttgact aagtcaattt tttgcaaaag ctcctggtta aaaattaatt
    26461 aaaaaaatgg ctgatgattt tatcgatatt gctactgacc caatacggca aatgcatgga
    26521 caattatttg attcaaattg attagaatga tataaagtgc acccgtaatg gctgcatcgc
    26581 ctaatgtgat gcgccaggag attaaataat ataccacttt taaaaggcta gacttttaat
    26641 ttgacaagct tatgttgtat tcactgatta aaaaatacca tactgcacac cgcctatccg
    26701 actattctta gcaatatccc gctatttttt atttctatcc aattatttca acgcactgtc
    26761 tctttaatta acgtaaagag caatatcagt ttttttaata tgtaaaggat gtcaatatgc
    26821 atgtgttaga tcccttaact cttgaagaaa ttcaggaaag ttgtgatatt ttaaaacgag
    26881 aaaaagagct ctccgacagt tatcggtttg cctgggtgat gacttacgaa ccgcctcaac
    26941 aagaagtttt gcattcaagt gataccgatt tcgaccgttg tgcttttctc tctgttttta
    27001 ataaaaaaac gaatgaaacc tttgaagcag tcgtaaacct caacagtaaa aaagtcgtgg
    27061 aatggaaaca gattgtcttc gaagccccgc cttatggtaa gccgcctatt ttaattgagg
    27121 attttcaaaa atgtgagcaa atcgttaaag ccgatccgac ctggagagcc gcgatggaaa
    27181 agcgtgggct tgatgatgaa caaattgaca acattcaagt agacgcattt tctgccggct
    27241 attttgatga agaggaggag agagaacaac ggattgtcaa agggataagt ttttaccgtg
    27301 ataatttaaa ggataatggt tatgcccgtc ccattgaagg ggttgtcgcg atagtagacc
    27361 ttggcaatga gaaggtattg cgtgtcattg atgacggccg caacgtccca attcctaaag
    27421 aaaaaatcaa ttacgattcg gattcttatc ctgaaaaacg caaagactta aaaccactgg
    27481 atattattca agcagaaggg ccaagtttta aagtaaacgg ctgggaagtc aattggcaaa
    27541 attggtcatt ccatgttggg tttacgccgc gtgagggatt ggttttgcac cagatcaaat
    27601 acctggatga agggaaagaa aggccaatta tttaccgggc cagtgtgacg gatatgatag
    27661 tcccctatgc ggacccttcc gtgagtcatt actggaaaag cgcttttgat gcgggtgaat
    27721 atggtcttgg caaattggca aatgaactaa agctcggatg tgattgttta ggcaacattt
    27781 attattttga cgttcctgcg gcggatgact tcggcaattc cagattgatt aaaaacgcga
    27841 tttgcatgca cgaggaagac gcgggcaccc tgtggaagca ttatgaaaca cgcaacagca
    27901 cctttgaggt tcgccgtgct cgtgagctgg tcgtcagctt ttttactacg ataggtaact
    27961 acgactatgg cttttactgg cgatttggac aggatggcag cctcaaactg gaaataaaac
    28021 tgactggtat tgtacaaaca gcggcaatat ttcctgaggc tcagtatgaa tggggaggga
    28081 aattgacacc ggagctggca gcacctacgc atcagcattt tttcaatgcc cgccttcata
    28141 tgatggttga tggtgaaaag aactcattca gtgagcacga gttttatcct atcgccatgg
    28201 gtggaaaaaa tccctatgga aatgcgtttg gcagcagcca gaaaacattt gaacatgaag
    28261 gtgaggcagc ccgctcggct tgtgcgcaaa cccagagaac ctggaagata ttcaatccca
    28321 atagcgtcaa tggcattggc acagcacccg cttataaact ggaattgcca gaaaccccct
    28381 tactgctggc agatgaagag agttatattt ataaacgcgg gggatttgcc agcaagcagg
    28441 tgtgggtaac acaatatgat cctcaagaaa aatacgcggc aggagattac cccaatcaga
    28501 gttccggggg ttgtggtttg cctgcgtaca tcaaacagaa ccgtaaaatt gacaatgaaa
    28561 atttggtttt gtgggtaacg ctggggtcta cgcattttcc aagacccgaa gactttccgg
    28621 tcatgcctac ttcgatcatt tctgccaagc tgcatccttt tggttttttt aagaggaatc
    28681 cggctatgga tcttcctcaa ggcaaagtac aggaaaaagg ggatacctgc tgttcttcat
    28741 taacttatta atggattgga accaagcacc ttcttgagga gagggtgctt tcctgtcttt
    28801 agttttcctc ttttttatcc agcttgtgac catccagatg acgttcactg cgttcattgt
    28861 cttgtttttc ttgttgcttt tctttttttg tccttccaaa ttttatgcga ttttcagatg
    28921 cttttatttc tttttctaag cggactttag cctttctttt tttattaaga ttaatgatgt
    28981 ctgccatggc actctctgtt gtaagacctt gtttatacta tagcgcataa agccagggca
    29041 aatcatctca atacacaaat cgatcgaaat ggaatgatct gaaagtatac actttgagaa
    29101 tgggaaggac tgtcgcaaga tagtattttc agcattgttt tttatgacac tttgcaaaaa
    29161 gaacaagcat ccttttaagt gcaggatttt agtgtataac cagagtgtat tcacagagtt
    29221 atccacagaa aatggggata agtaatcaat acaattcacg tattgatatt taataaatct
    29281 ttacagggca aggggtttta taaaaaaggc atggtgtcaa atactgatga gattaaaaat
    29341 aaagacaagt tatccacaga aagcgggatg ctagttaagg tatgggacgc tcattatgat
    29401 aacaaaaaaa aaaataaaaa acagtcttga ctggcacaat tttttacctg aaaatgattt
    29461 ggatagactg gcatcaggaa taaggcaata caaaaacagc taatcctgct ataatttttg
    29521 aacaaaacgt gagctcctgt tggcaggatt tttcacttta gatcgttcac tgacggcttc
    29581 tgaggctgat ggcatagagg gggttcaggt gattttctca tgagtcgagt tttcaaagga
    29641 gtatgaaatg agtaactacc tcgataaaac cgcaagctta accccagcca ctaaaagaat
    29701 agtgtgcaac aaagctactg aatatcctca tactggctct tataatcaag tagcgactca
    29761 tggcacgtat ttatgcaggc gctgtggcct ggcgttgttt cgtggtgtca gtcaatttag
    29821 ctcaggctgt ggctggccca gttttgacga tgaaatagct aacgctgtgg caaggaaacc
    29881 ggatgcggat ggtcagcgca ccgagatatt atgcgctcgt tgtgatgctc acctggggca
    29941 tgtgttcaca ggggaataca tgacgtataa gaatttacgt cattgcgtta attctgcttc
    30001 attggatttt gtcgcggata atgaagtact ggatacagaa gaagccatcc ttgcaggggg
    30061 gtgtttttgg ggagtggaat attatttaaa acgaattccc ggagtgttaa aagtcgagtc
    30121 gggctataca ggtggccatg tttcagagcc aagttatgag caagtatgca gtggtacttc
    30181 agggcattat gaggctgtgc gagtcgtcta taattctgct aaaaccaatt atcatgacat
    30241 tgtaaagcga ttttttgaaa ttcacgaccc aactcaggcc aatggacaag gtcccgactt
    30301 gggccaccaa tatttaagtg ccgtgtttta ttttaatcag gaacaactcc aggaagcgga
    30361 gttattgatt cagttattaa agaaaagagg gtatcaggtt gtaacccaat tattgcaagc
    30421 acaacccttt tggcctgctg aagactatca tcaagattac tattttaaac accacaaaat
    30481 tccttattgc cataagccag tcgatcgttt tggctagttg ttccggaaga tttaatctca
    30541 tcatagttgc acggatttag aaggcagaca actattggca tattcttcaa gatcatctat
    30601 cgcgcacgtg ttttcaaatt cttctgcact gcttgtcttc attttcaagt ctatggtgtt
    30661 tctccatttg agcgctttct tcatgattct ttcgttgcgc tttatgttct ctggtgtttg
    30721 gggttggtct tcaaaaaaac tacagtgctt tagaagagag tgcttgaagg tttcctcttt
    30781 agaattaatt aatggggtag aattcaggac tttatcatct gtgatgatgg ctcggctttg
    30841 tgcaccgcgt aatatccgag ggggcaaact ggcacaatgc accggaaaat cagcatcaag
    30901 ttttgcaatt tttaccttgt cacttacctc agcttgttta tccataaaat cataaatatt
    30961 ttcatattta tcctgggtag acaacatgct ggcgcaatcc atagtaaagg tggcgcaccc
    31021 tgcgcgcgag tgctgtaatt taacatcagg gatgtagatt tttattgaaa gatcttcgtt
    31081 ttcatcaagc aaatcctgga atccaacttc attcgctaat atttgagcag tgttatagga
    31141 taattgtgct cccaaactgt caagcaccaa aatttttaaa gtattattgc ccttttcaat
    31201 ggaatattgt agctcaatta atgaccaatg gcgcacaccg gatttttctc tatccagata
    31261 ggcaatttgt accttgaaat tgttttcaaa taattttggt gaatgacaac tgctataaat
    31321 aaaatcataa agcttatcac ggcgttcgta atcacagaca aaaccggtaa atggataacc
    31381 tgattccttt gattcccgat attttttctt taaactaaag gccacttcca aatcatgtgc
    31441 ggaaaatg
//
LOCUS       DACTJE010000019        29966 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.019, whole genome shotgun sequence.
ACCESSION   DACTJE010000019 DACTJE010000000
VERSION     DACTJE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 29966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29966
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.019"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(16..225)
                     /locus_tag="I9760_000662"
     CDS             complement(16..225)
                     /locus_tag="I9760_000662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042451.1"
                     /translation="MHQWVSWWTRTANDWTKHNLLTWFIDTCWDASAKEVARMLLHRL
                     DGAQAALANSDHSYVNQALVLVQAQ"
     gene            complement(427..991)
                     /locus_tag="I9760_000663"
                     /pseudo
     CDS             complement(427..991)
                     /locus_tag="I9760_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005506484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1157..4309)
                     /gene="putA"
                     /locus_tag="I9760_000664"
     CDS             complement(1157..4309)
                     /gene="putA"
                     /locus_tag="I9760_000664"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HAT5042452.1"
                     /translation="MLEKQSIHLPEGLRAAINKAYRMDELSLITELSEQAALDPQQMM
                     AIKTSATKLVQSVRSERKKSTGIDSFLTEYALSSDEGIALMCLAEALLRVPDNATIDN
                     LIKDKLAGGDWGAHRGQSESFFVNATTWALMLTGKVLTPEKAENTLTKALLKLVNRSS
                     EAVVRKAVDKAMRIMSKQFVMGRTINEALARAKKKEDRGYRYSYDMLGEAALTSADAA
                     RYFEAYKEAIISIGEKADKHSDVYRRPGISIKLSALHPRYSEFQYERVMAELPPKLLA
                     LSRLAKDYGIALTIDAEESERLDLSLDVIEKVFTDESLQGWNGFGLAVQSYQKRAFYV
                     LDWVAALARSKQRRIMVRLIKGAYWDSEIKKTQMQGFSEYPVFTRKVFTDVSFQACAK
                     KILTMTDAIYPQFATHNAYSVAMILNLVGGYRDFEFQCLHGMGNELYEQIVPANCYGI
                     PCRIYAPVGSHEDLLPYLVRRLLENGANSSFVNRIVDDKAPISELVEDPVAKSRSLLD
                     KINKNIPLPEDIFLPVRKNSKGFDFTNRLERALLQQELAKIESKEWQASPMIAGRKLS
                     RDLLQTVMSPQQPAYAIGSVQQATLDDVEVALNQAKLAFELWSKKPVEERASCLNRFA
                     DLLQANMAELMVLTCREAGKTWSDGIAEVREAIDFCRYYAKKAQELMSSPQRFNGYTG
                     ELNELSLHPRGTILCISPWNFPLAIFTGQVVAGLVTGNCVIAKPAEQTPLIAAYAVKL
                     MHQAGIPEGVIQLIPGAGETIGAALVADKRIKAVLFTGSTDTANLINRTLATRGGEII
                     PLIAETGGQNAMIVDSSALLEQVVVDAVTSAFGSAGQRCSALRVLYVQEEVYPRTVEL
                     LKGAMAELVVGDPQWLSTDVGPVIDKEALSILKNHVENMRKHHEILYQCTVDDEALSG
                     YFMPPTAIAIDSISALEKEVFGPILHVIQFKRKDLDKVINQINQTSYGLTLGIHSRIN
                     ETVDYIRQRVHAGNCYVNRNMIGAVVGLQPFGGEGLSGTGPKAGGPNYLIRLCHERTY
                     TVDTTAAGGNASLMSIPEEG"
     gene            4866..5543
                     /locus_tag="I9760_000665"
     CDS             4866..5543
                     /locus_tag="I9760_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HAT5042453.1"
                     /translation="MNTLFTLISTVLLTSNITSSTPLFAKPGTDINTQVQHLSHKAPQ
                     LNKKVLKLALTAYKNASKRGAVKKPVLTVIDYSLPSNKQRMWVFDLRNERLLYNTYVA
                     HGKNSGVNVARSFSNRESSKQSSLGTYITKNTYIGHKGYSLNLQGLDRGFNDNAYNRR
                     VVIHGAWYVEPEFIKKAGRAGLSWGCPAIAQTLAKPVINTIKNGSVVFAYYPDKKFLS
                     HSGYLVA"
     gene            5969..6340
                     /locus_tag="I9760_000666"
     CDS             5969..6340
                     /locus_tag="I9760_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein convertase"
                     /protein_id="HAT5042454.1"
                     /translation="MIMNQQLNATKKDKLQVIDWLIENFPNAFFKKGNQVKPLKIGIF
                     DDLIDFYERLDTPPFSKKSLREALSYYSASPAYLSCQKPDTARVDIYGNEVDVVTPEQ
                     AKYAYQRYQERYGNKKSQELK"
     gene            complement(6368..7060)
                     /gene="ubiG"
                     /locus_tag="I9760_000667"
     CDS             complement(6368..7060)
                     /gene="ubiG"
                     /locus_tag="I9760_000667"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HAT5042455.1"
                     /translation="MNQIESTIAIEEVHKFAQLANDWWDTNGPLRTLHDINGARFEFI
                     SEHINLKGLRVLDVGCGGGILCESMAKAGAYVSGLDAEPEAIQIAKEHAHKNQLQIDY
                     FCNPIEEYEEQGFDVITCMELLEHVQNPELVLQHCRRLLKPNGLLFLSTISRTLKAYL
                     GAIIAAEYVLNLLPRQTHDYDKFIKPSELVKMARLYDLNLIDMKGLCYNPFLRKTTLV
                     SDVSINYIMVLQ"
     gene            complement(7047..7766)
                     /locus_tag="I9760_000668"
     CDS             complement(7047..7766)
                     /locus_tag="I9760_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAT5042456.1"
                     /translation="MYENLLVGCHEEWKEILMKALNTMDSDYLKQIQEDSHWLPGLNQ
                     LFSAFSLPLSQTQYILLGESPYPRKISANGYAFWDNSVGLLWSSTGLSKEVNRATSLR
                     NWIKTLLIARGDLHGNTSQEAIAKINKSCLVQTAEDFFKGMMKKGILLLNASLIYSKG
                     KVPYHARHWKPFMQNLFEQLAIRKPDVQLILFGKIAEKIPANKLLIGLVSEHPYNVSF
                     ITNQRVIEFFKPMDLLSYESN"
     gene            8037..9713
                     /locus_tag="I9760_000669"
     CDS             8037..9713
                     /locus_tag="I9760_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinectin 1"
                     /protein_id="HAT5042457.1"
                     /translation="MFLANCNIEELVTEHIKQFLADEELSFSGLKDLILSKAPIPWIH
                     SSVTATLLKSRDSDKTEVKKNLEQQSYKAQLAEDKIQKEQDDAEALKDKKLKEVLTRE
                     LNHIPTQISEQQTELRLLHYKLERLFESQAKVDVIQHPDSPMKKIKPSSSHSASIERL
                     QRSINEREIKIQSLFEQEVNNKIKLNEIEKRASVRSQHHTKRVKRAQARIGYNSTGED
                     ILSTLSGKNQSILLRSIQKQHHALEKKCSDLIQEADQINYPLFLEELQKYLNKKKHTL
                     SSQEVDALKSVIKYIKQHLEFEHEVINTQSSLHIKKQSISSQIVKLRELQNKLKTLKN
                     NNPNLTAANEELVSRNLELTAMKEHHADLRHRLGTPALLLFGLTFLFSIPLILTISGV
                     IPFFIAPALLYILVSAPPTILLLSTLGVGIAAIVFSFKMHSNESAIKSNLQTIETNSN
                     QMSRNSQNLKSLETLTIPTLDMQIKKDENLRDQLMLSLQKQQRQAAQAFQKAKEVECL
                     SYATSSLLNSGNTQSSDPSLSTHSEESDEGLNDSNDSLQALEEETVNEIA"
     gene            9849..11489
                     /gene="ppeB"
                     /locus_tag="I9760_000670"
     CDS             9849..11489
                     /gene="ppeB"
                     /locus_tag="I9760_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PpeB"
                     /protein_id="HAT5042458.1"
                     /translation="MSLATYDVKQELVKLTQNFFSIDHQFSELKNLLTDKKTIPWIHN
                     QIVDQLSQIKKSDEIAMIARLEKSAYEQQMKEDDDEKNRDVEELRQDLLRRNDLTRQL
                     EILRVRKEQYERELLIRNTVPHVHTHPDTPVIHQHPSQPISAPLLNPNNHVHTHLEAT
                     LSSQDIELSVRKINRRISEINKELEILDSRNNAREIRRGDREKRLQARLNYVQKTAGV
                     VNTLSPDNQKKLLSNIEKEKKSLTQQHSSLLLKADQLNYSIFLQQFELSLQSMQRPFQ
                     EADALKAIVKRMKEHLNYKEKAASIQSRLDNTVLTIGENLRRLTRLNTQLSSLQLANP
                     DLTRRNERLEEQNRELLESYNSHIKTRNKLFFPTLVITGLSLLFSIPLILTLTGIIPY
                     AIAPAVLLTLVIAPPALLLLAGLGVGIATIIYAVKAYFNNSTIESNQETIESNRRQMG
                     ANQKEIYTLENQTIPNLKKELLENEEIKNRLTDELQYIENLAEQALKQANEVEPYVYS
                     AMPFFNPEVVVHQHPSVGNGIVTPSAPPIDPLYPAVRH"
     gene            complement(11576..12832)
                     /locus_tag="I9760_000671"
     CDS             complement(11576..12832)
                     /locus_tag="I9760_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAT5042459.1"
                     /translation="MFALVDCNNFYVSCERLFKPALANKPILVLSNNDGCVIARSNEV
                     KALGVQMGVPYFAVKALCKQHKVHVFSSNYTLYADMSRRVMSVLEDNWPAIEIYSIDE
                     AFLDLNTLPQSEHDEFCRKLQKIVLRHTGIPVSIGIGATKTLAKLANHIAKKELRTPV
                     FNIENQYHWLAKIVVGEVWGIGRQWGKKLVQQGIYTAHDLAGMDLQLIKKLFSVVLQR
                     TVLELRGVSCGSLQDFESRKSIISSKSFGSMQTEYSALAQAISSHCARAWEKLRHQKL
                     MACHLSIFIKTNQFRLDLPQYQQTIGFKLINPTDDLRHLIRCAKLCLRKIFRSGFHYQ
                     KVGVYLGDLIEKNCLQLDLFNQVSEKELNQTERLMSVLDEINTKYGRHTLRLAAEGHV
                     KPWAMRAQSRSPRYTTSWSELPVVII"
     gene            complement(12832..13356)
                     /gene="umuD"
                     /locus_tag="I9760_000672"
     CDS             complement(12832..13356)
                     /gene="umuD"
                     /locus_tag="I9760_000672"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAT5042460.1"
                     /translation="MSHGGARAGAGRPRGQGKYGESTKSVRIPESRIQDVMKYLQGEK
                     EFPAIPLYTSSVRAGFPSPGDDYIESMLDLNEYLIKHPASTFFVRASGESMINAGIYS
                     GDILIVDRGIEATHGKIVIVALNGELTVKRLSRQHGQVKLLAENPDFPPIDITEEYDM
                     VIWGVVTHVIHTVL"
     gene            13522..14745
                     /locus_tag="I9760_000673"
     CDS             13522..14745
                     /locus_tag="I9760_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT5042461.1"
                     /translation="MVKLYKEIINLIDNHQATMVEQLHQFCEINSGTTNLTGLAIMAE
                     TLCSAFKPIADTTELITLPPLSVIDMTGNTVMQSCGDVLFISKRPHLKRRILLSGHMD
                     TVYSASNPFQKLSYLDANQINGPGVADMKGGLIVILHALAAFENTRFAEDMGWDVLIN
                     SDEEIGSPTSGSLFNKLAHRYQAALVYEPAMTPNGTLAKNRKGSGKLTLVATGRAAHA
                     GRAFDEGRNAICYLAEAITAVHALNGQKNGVTINVGKIAGGEALNVVPDKAVAQLDIR
                     ISLPEDEIWVRNELNKIIKQLERPDYSLNVHGTFGRPVKRICAGTERLFHRIQKIGEA
                     LGLTIDWKDSGGCCDGNNLAHYGLPVLDTLGVRGGNIHSSDEYILLDSLSERAALSAL
                     LLIDLAQGGLEDLIK"
     gene            14742..15785
                     /locus_tag="I9760_000674"
     CDS             14742..15785
                     /locus_tag="I9760_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAT5042462.1"
                     /translation="MMLFRSARDSDLDAIHQLAEISGVGMTTLPKDKELLKKRLEWSC
                     ESYQRNVTNPGCEYYFFVLEEQKNNTIVGTSAIEARTGYEIPFYSYKLSKRTRVCHTL
                     NIRSDYEVLSLVNDNQGRTEICTLFLHPDYRKNNNGLLLSKGRFLFMAQYPHRFAPIV
                     IAEMRGISDDKGNSPFWDNVGANFFHMPFVEADRLTLATNKQFIADLMPRNPIYVKLL
                     SPEAQAVIGKPHQSTIPAMNILLKEGFRYNNYVDIFDAGPTIESPLLEIKTISLSRIM
                     VIKNISDEASSSNRYLLANTQMDFRATISHAVFNERENTCIISKETAKLLDVKQGDSL
                     RISPIRTEDTPPL"
     gene            15799..17286
                     /gene="astD"
                     /locus_tag="I9760_000675"
     CDS             15799..17286
                     /gene="astD"
                     /locus_tag="I9760_000675"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HAT5042463.1"
                     /translation="MSKLQIIQSKGQYINGEWIKGNGLILESTNPASGTLLWQGNNAT
                     DEEIANACYVAHRALKSWANTSFEERARYTKAFVEQVEKNREQLARLISLETGKPLWE
                     SQTEVSSVIGKVNLSIQAYQERTWPKQTETAEANACLRFKPHGIVVVLGAFNFPAHLS
                     NGHIVPALLAGNTVLYKPSEHTPAVAELIIQCWHDSGLPPGVINCLQGNANCGNTLLS
                     QDIQGVYFTGSYATGLRIHQQFCNRPEVILALEMGGNNPLVIDEVKDIDAAVYHTILS
                     TMITAGQRCTCARRIIIPDSQTGDLFLERFAKACKLMRIGSFDSQPEPFIGPVISHVQ
                     ALKHLHAQKQLIEMGGEIILPMSLLLEYTGLVSPGIIDMTRAKNPPDEEIFAPFAQIY
                     RYNHFDEAIQLANQTRYGLSAGLLSDNKDHYQQFYQNIRAGLINWNRPTTGAASSLPF
                     GGVGCSGNHRPSAYFAADYCAYPVASMEQPLLTTPVQRLPGLVLE"
     gene            17292..18638
                     /gene="astB"
                     /locus_tag="I9760_000676"
     CDS             17292..18638
                     /gene="astB"
                     /locus_tag="I9760_000676"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAT5042464.1"
                     /translation="MNVYELNMDGLVGQTHHYAGLSSGNIASTNNALSISNPQAAARQ
                     GLEKMRQLYNMGLKQGLLPPHQRPNLNLLYQLGFKGTPSEQINKAYKTAPELLSACYS
                     ASSMWTANAATVSASVDTEDNKVHFTAANLISNLHRHQEADFSKKLLEFIFSNSDYFN
                     HHPLLPKSMGTSDEGAANHNRLCQSHAHSGINLFVYGKKVLGNHQFEQSPIKYPARQT
                     KEASEAIARNHLLNPERVIFACQNPLAIDQGVFHNDVISVANEHVFLVHEEAFYNQAY
                     VLDQLREKADFPLVIIQISKEQISVSEAVDTYLFNSQLITLPDQKNMILIAPAECQAN
                     LKVKTCIDGLVADPQNPINSVYYLDLKQSMRNGGGPACLRLRVPLNDYELKAMHQGIL
                     IDNDLLDILDKWVLKYYRTELKIPDLADPQLLYECLDALDELTQILKLGSIYPFQS"
     gene            18650..19375
                     /locus_tag="I9760_000677"
     CDS             18650..19375
                     /locus_tag="I9760_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042465.1"
                     /translation="MWKTKFGKCIYISPSGYKVYQNLLYRWLTLGSEAWQTVINRYIP
                     RRPGLHYLQILTLMARKFPNDCCLLGLGGAGIAHLLGAQKTIRSIVAVESSEEVIQIA
                     KKFFMTDLIPHFTITHQNARDFVRENKKTFQHMLLDLYDATHFPDECHSMDFFIHCKK
                     QLTDDGFLAVNLANSQEQWALVQLIKKQFPSNIIIPIKKCANIVVIASKNDNKDLFIK
                     QVRNTNEIKKITWVKDWGYVAEY"
     gene            complement(19453..19818)
                     /locus_tag="I9760_000678"
     CDS             complement(19453..19818)
                     /locus_tag="I9760_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAT5042466.1"
                     /translation="MKRNALIIMLVYLTSTPVFAKNLGKYTYEIACKNCHAPNLAKAI
                     KAPPAFDKKAWKLRFKQAKIEAKNNPSQFESAMDYLLYNVKVGKGLMHHGGLCKEADV
                     PNTDCSDEALIEAINYMRK"
     gene            complement(20196..20753)
                     /gene="frr"
                     /locus_tag="I9760_000679"
     CDS             complement(20196..20753)
                     /gene="frr"
                     /locus_tag="I9760_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003637067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAT5042467.1"
                     /translation="MINEIKQDSEKRMKKTIEALHTDMSKIRTGRANASLLDHVMVDY
                     YGSPTPLSQVANITTSDSRTILVTPWEKSMVAAIEKAILNSDLGLNPATAGTAIRVPM
                     PPLTEERRKELIKVVRHEGEQGRVSIRNIRRDANNQLKELVKEKAISEDDERRAAEAI
                     QKLTDRYISEVDAVLAEKEKDLMEI"
     gene            complement(20743..21486)
                     /gene="pyrH"
                     /locus_tag="I9760_000680"
     CDS             complement(20743..21486)
                     /gene="pyrH"
                     /locus_tag="I9760_000680"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAT5042468.1"
                     /translation="MMNESQPKLKYKRILLKFSGEALMGKSQFGIDPSVLDSLARDIA
                     ELIHMGVEVGLVLGGGNLFRGKALSQAGVGRVTGDHMGMLATVMNALALRDALERIDL
                     PARIMSAIPMLGVVDPYHRRKAITHLRNGQVVIFAAGTGNPFFTTDTAACLRAIEIGA
                     DIVLKATKVDGVYSADPLKNSDAKRYDYLTYKEVLTKGLEVMDSTAICLCQDQGMPLQ
                     VFDMAAPKALKRIVTGERVGTIVGANHDQ"
     gene            complement(21483..22361)
                     /locus_tag="I9760_000681"
     CDS             complement(21483..22361)
                     /locus_tag="I9760_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAT5042469.1"
                     /translation="MSTISAALVMQLRERTGAGMMECKKFLIATNGDIEQAIIEMRKA
                     GQAKADKKADRVAAEGIIVIARSSDERTAVMLEINSETDFVARDENFTNFANAVADVA
                     LTSLPKNIEDLSNQALSSGATVEQARQELVAKIGENIKLRRLEKMHCDGVIGYYLHGS
                     RIGVMVALKNGSEALAKDIAMHVAASKPMVVSRDQVPAEAIENEREIFTAQAKESGKP
                     QEIIDKMIDGRINKFIDEVSLLGQPYVKDPNIKVGQLLKEKNAEVISFVRYEVGEGIE
                     KKEDNFVEEVMAQVRT"
     gene            complement(22427..23191)
                     /gene="rpsB"
                     /locus_tag="I9760_000682"
     CDS             complement(22427..23191)
                     /gene="rpsB"
                     /locus_tag="I9760_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAT5042470.1"
                     /translation="MNNVSMRELLEAGAHFGHRTRFWNPKMSEYIFGSRNKIHIINLE
                     KTLPMLSDVTNYVSRLAANKAKILFVGTKRAAQDSIREHAKRCGMPYVDHRWLGGMLT
                     NYKTVRQSIFRLKELKEMKEKGLFNDMIKKEALMLTRELEKLERSLGGIENMGGLPDA
                     LFVVDVGFEHIAVEEARRLRIPVIGVVDTNNSPDNIDYVIPGNDDSMRAVDIYVRCVA
                     DAILDGKNSNTVGRVSSDSEFVEVTSNSNEEEKSGE"
     gene            23525..23935
                     /locus_tag="I9760_000683"
     CDS             23525..23935
                     /locus_tag="I9760_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HAT5042471.1"
                     /translation="MSKKSICLATLALIFTFGQPVFANSSEATQTKPCHCHDHKKILS
                     QLNLTQEQKTKIKTIKIQAQKSIKENYRQLKAIKEQINALIANEKLDEAKLDSLINQR
                     NKITGTMLKNRVMMKNQIYNQLTNEQKSQYKKNM"
     gene            24445..24903
                     /locus_tag="I9760_000684"
     CDS             24445..24903
                     /locus_tag="I9760_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1716 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042472.1"
                     /translation="MRGHMIFLSIPKGMEFKQITEKDNTNDYFVDPNGKLPRINIQAL
                     VKDALQYNKGRKKEISLPDFTIYRHKPPYRDELFLQYNPDHNGKYFTKESVNLVNGKE
                     FIKYKTPATSYGTFWFQKVQLSESRMDEVLAKRSEQRENRRHTGDSPNPT"
     gene            complement(24967..26655)
                     /locus_tag="I9760_000685"
     CDS             complement(24967..26655)
                     /locus_tag="I9760_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1717 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042473.1"
                     /translation="MPVRYSGFDYDGCVGVTNLINIVAQNPKLAQRIRETSNYYTKEV
                     ALIASNRQSDRDDKRNARSNGNGSCFIQIQKLCTDLGMEFDPFLLADAYNLVPAGTSL
                     HEATNPANGAKIQNGWLHDKSKASLVYAHMQRASSLEAREQIDYFFYDDREDILEGLY
                     QFFSANPELIPSNVTMHLVKYACSRENVHTMDNYKIPVQGKGFTNPDYVKTIKAFGDK
                     AIENARDVYDIDGKEINAAGVRQAKYNCNFEFEANKLQPMKFVNNFNSPAELIKAHEQ
                     AIEKIKLGAFELYKAKINVITTLSNEQKVFLIRAKAQQWNLLCDEGQWNNPIFTLQAI
                     MAEKSDRFFLPQYAFTAKMLIKNYQEARENSKDGSRFWEQINAFIKSMNALKDPMRNA
                     QSIFEVFKKNSYSVKAVDGFVKKVVEVMNALMDKYPQRSTTEMVACMRILESNLQKMV
                     NFFSLVDRLITIYRGCTTAGLNFNKILTVVREALEEMKMITDSGDNYSTTLIDLDKVI
                     TAANNRLESLTYPKEYSTKNFHSQESYKVVGAYLCMQSQLIQKLDTKASTALAI"
     gene            complement(27046..28683)
                     /gene="ankI"
                     /locus_tag="I9760_000686"
     CDS             complement(27046..28683)
                     /gene="ankI"
                     /locus_tag="I9760_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkI/LegAS4"
                     /protein_id="HAT5042474.1"
                     /translation="MIILYDFLQESSVMPRSKNDSKLKKKSALQSKFKEQQLNHGSKE
                     HKSKFKFSQRKAKKKGPGMTHLPGNIYTLFTPVNGPKSKDEWIDTSKIMLDLHIDNMS
                     SSDYIPSAIDRTDLVMVQSVHLLRKTGGRGLFAREDIPKGTCIGIYTGEVYSEQEFEQ
                     YLKEHVGSDKSYAMYVGGRVIDAARKGNLTRYINFSDSQDNAEFVETTLNRKKVAKVI
                     TTKNIKAGQQLLINYNTYEEQASRYYYFLNPGDGWLSAQEFYQTYQSQYRLEQMPYNL
                     EGFDLKAGDRVLMTQIGRIILANYSLAKEQELNASDIDLPFLKVGSDEKILDFDEADT
                     FTPLMAACYLGQVENVKWLIEHGANIDQQQSHSGHCPLSLTLKGYSLAKDTQKYIDII
                     QLLIKNQVNLLVHDRSDKTFLHNAALVLNNLDFQSVVKFLIGQNPIDINEYFTYIDEN
                     DFDIVMHCYNNKLFDKALVLLAFYPDYFKRNYMSDNEGHNQFNINAFRKAIKDFNSNE
                     RSILLMQLRESGLHLPEDLLEQLGIMDSSITLESKFF"
     gene            28827..29591
                     /gene="map"
                     /locus_tag="I9760_000687"
     CDS             28827..29591
                     /gene="map"
                     /locus_tag="I9760_000687"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAT5042475.1"
                     /translation="MGVTIKTPDEIEKMRVAGRLAAEVLEMIGPYVQVGITTDELNTI
                     CHDYIVNVQKAIPAPLNYNGFPKSICTSINHVVCHGIPGKKTLKDGDIINIDVTVIKN
                     EYHGDTSKMFIVGTPSVKAKHVVQIAHECLYIGIDMVKPGVQLGDIGHAIQQHAEKNR
                     CSVVRDYCGHGIGRVFHEDPQVLHYGVPGTGMKLEPGMTFTIEPMVNIGKHHTRLLPD
                     HWTVVTKDHSLSAQWEHTLLVTDTGVEILTLRNEER"
     gene            29578..>29966
                     /locus_tag="I9760_000688"
     CDS             29578..>29966
                     /locus_tag="I9760_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein"
                     /protein_id="HAT5042476.1"
                     /translation="MKNDNRIIKNTIKQFKEKLCKDFSQKTNITSITRKLAVFIDTIL
                     IQLFIKNKLHFGDNFCLLALGSYGRRELQLHSDIDLLILHTEKVSNIQLQRAQKFIQD
                     CWDVGLEVSHQITTVSSCANLASQDLSV"
BASE COUNT         9482 a         6158 c         5202 g         9124 t
ORIGIN      
        1 acgcaaaagc aaatattatt gagcctgaac aagaacaagt gcctgattta cgtacgagtg
       61 gtccgagtta gctaaagccg cttgtgctcc atcgaggcgg tgaagcaaca tccttgcaac
      121 ttctttcgcg gatgcgtccc aacaggtgtc aataaaccag gttaataagt tatgctttgt
      181 ccaatcattt gctgttcgcg tccaccagga gacccattga tgcaagtaac tcatggctcg
      241 aagggaggtg tataggttcc attgataatg gattgcaccc cttgagttaa ccattcactg
      301 atagtacgag ccattaagtt tgtgatgggc aaacttcttg cgcttatggg tgcttgcaaa
      361 aagcttgcta ccggtattcg tcatctctac aaaatcccag cgtttcatga tgctccaatg
      421 tcaaagtcac tggaaattat cattgatact ttgtcctaat gatgcaccga tatcaggcag
      481 tatttcagta aaaaaccgtt caatttctct tctaaaatcc ttagcactgt tgaaaaatcg
      541 attgtttctg accttttcat tcatgacttt ccataatcgt tcgatagggt ttaaattggg
      601 actataagga ggaagatagt ttaagtgaat aactaatttt ttagcctttt ctttgacttt
      661 atctgctcga tgataagggg cttggtcaag tattaaatga atgattcctt ttgaaccatg
      721 gtttgctctt atcagactgc tcctgctgtt ctctattcgc tttatgtgga tgacctttag
      781 gtttcttgta gctaaagcca ttacgatgca tccatttatt catccctgga acactataga
      841 taatagacca tttcttctct acataggcaa taatataacg caataaaaca gcttttatac
      901 gatcacgctc cttaccgtta gagcaacaac tattccttgc ttccagccct tcttttcttc
      961 tggtgataag tgtattcttt tcatgcggca tagcatgatc ttctcagatc aaaaaagcaa
     1021 gcattttcat agatcagcgg tatagatcag ccctcataca agaacaacca aatattgccg
     1081 ttccagacag gggcagatct cctgtaataa attcacttaa gattttatta ttctcttaag
     1141 tgaatttcta atactgctaa ccttcttccg ggatagacat taaactggca ttgcctcctg
     1201 cagcagttgt atcaactgta taggtacgct catggcaaag tcgaatcaaa tagttagggc
     1261 ctccagcctt gggtcctgtg ccagataacc cttcaccacc aaaaggctgt agacctacta
     1321 ctgcgccaat catattacga tttacataac agttccctgc atgaaccctt tgcctaatgt
     1381 agtcaactgt ttcattaatt cggctgtgta ttcccagtgt taaaccatag ctagtttgat
     1441 tgatttgatt aattacctta tccagatctt ttctcttaaa ctgaatcaca tgcaaaattg
     1501 gcccaaatac ttctttttcc aaagcgctga tactatcaat tgcaatagct gttggcggca
     1561 tgaaatatcc tgataaagct tcatcgtcaa cagtgcattg atataatatt tcatgatgtt
     1621 ttctcatgtt ttcgacatga tttttcaata tagataatgc ttccttgtct ataacagggc
     1681 caacatcggt tgataaccat tgaggatccc ctacgactag ctcagccatg gcacctttta
     1741 atagctctac agttcttgga tacacttcct cttgcacata caatacccta agtgccgagc
     1801 atcgctgccc cgcgctgcca aaggcagaag tgacagcatc aacaaccact tgttccaata
     1861 atgctgaaga atcgacaatc attgcatttt gaccacctgt ttcagcaatt agaggaatga
     1921 tttcaccacc tcgagtagct agcgtccgat taatcaaatt cgccgtgtca gtcgagcctg
     1981 taaataatac tgctttaatt cgtttatctg cgaccaaagc agcgccaata gtctctccag
     2041 cgcctggaat taattgaatt actccttcag ggataccggc ctgatgcatt aacttcacag
     2101 cataggctgc aattagtggc gtttgttctg ccggtttcgc aataacacaa ttgcctgtaa
     2161 ccagcccggc tacgacttgt cccgtaaata tagccaatgg aaaattccag ggactaatac
     2221 acaatattgt cccgcgagga tgcaaactca attcatttag ttccccagtg tatccattaa
     2281 agcgttgagg gctactcata agctcttgag ctttcttggc atagtatcga caaaaatcaa
     2341 tagcctctct tacttcagca atgccatcac tccaggtttt accagcttcc cgacaagtca
     2401 ataccatgag ctctgccata ttggcctgta ataaatctgc aaatcgattt agacaagaag
     2461 cgcgttcttc tactggtttc ttgctccaca attcaaaagc caattttgcc tgatttaatg
     2521 ccacctctac atcatcaagt gtggcttgct gcactgagcc aatagcatat gccggctgct
     2581 gtggggacat aactgtttgc agtaagtccc tggataattt tcgaccagca atcataggac
     2641 ttgcctgcca ttcttttgat tcaatttttg ccagttcctg ctgcaataat gcgcgttcaa
     2701 gtcgatttgt aaagtcaaag cctttagaat tttttcgtac gggtaaaaag atatcctctg
     2761 gcaatggtat atttttattt attttatcaa gtaacgatct tgatttagct accgggtcct
     2821 ccactaactc gctaataggt gccttatcat caactatgcg attgacaaaa gatgagtttg
     2881 ctccattttc cagtaatcgt cgaactaaat acggtaataa atcctcatgg ctaccaactg
     2941 gtgcataaat acggcatgga ataccataac aatttgctgg aacaatctgt tcataaagtt
     3001 cattacccat accatgcaga cattgaaatt caaaatctct ataacctcct accaaattaa
     3061 gaatcattgc tacagaataa gcattatgtg tggcaaattg tggataaatg gcatcggtca
     3121 tagtcaggat tttttttgca caagcctgaa atgacacgtc agtaaatact ttacgggtaa
     3181 aaaccgggta ttcagaaaag ccttgcattt gcgttttttt gatttcacta tcccaatagg
     3241 ctcctttaat caatctgacc ataatacgcc gctgcttact tcttgctaaa gcagcaaccc
     3301 aatccaagac ataaaaagct cttttttgat aggattgaac ggccaaaccg aacccattcc
     3361 atccctgtaa actctcatca gtaaacactt tctctatcac atcaagcgaa agatctaaac
     3421 gctctgactc ctcggcgtca attgtcaaag caataccata atcttttgct aaacgagaca
     3481 aggctaataa tttggggggt aattctgcca tgacacgctc atactgaaac tcgctatatc
     3541 gtggatgtaa ggcagaaagt ttgatggaaa taccagggcg tctatataca tctgaatgtt
     3601 tatcagcttt ctctccaatt gaaatgattg cttccttgta ggcttcaaaa tatcttgcag
     3661 catcagcaga tgttaaggca gcttcaccaa gcatatcgta agaatatctg tatcccctgt
     3721 cttccttttt tttcgctcga gctaacgctt cattaatggt acgtcccatc acaaattgtt
     3781 tgctcataat tcgcatggct ttatccactg cttttctaac aacagcctca ctacttcgat
     3841 taaccaattt tagtaaagct ttggttaatg tattttcagc tttttcagga gttaaaactt
     3901 taccagttag cattaaagcc caagtagtag catttacaaa aaatgattca ctctgtccac
     3961 gatgcgcccc ccaatcaccg ccagctaatt tatctttaat caagttatct atggtggcat
     4021 tatcaggaac gcgtaataaa gcttctgcca gacacattaa ggcaattccc tcatcacttg
     4081 ataaggcata ctccgttaaa aaagaatcta ttcctgtgct tttctttctc tcactacgaa
     4141 ctgattgaac caattttgta gcactggttt taattgccat catttgctga ggatcaagtg
     4201 ctgcctgttc actaagctct gtaattaaag aaagctcatc catgcgataa gctttattaa
     4261 tagcagctct taatccttcc ggtaaatgaa tggactgctt ttccagcata atgttctcca
     4321 agactaaagt ggtaattgaa aaaaatagtt attaaatttt taccatataa gacaaaaatt
     4381 aaattcatta ttataacaat gaaaaaatcc ggttatgata tcttacactg gcttatttgc
     4441 catgcccctc tctacgacaa ggcaatattt cagatataaa tcatagtatc gagcaataaa
     4501 ttgggctata taaaatcaat gcatcaaccc cctttttgtg gcatattata tgacgacaat
     4561 cctgttcgat aaagtaaaaa catgcttttt aacccagaaa aatagaacta aatttaacca
     4621 acaagatcta attataatca ataagatgtt tttttggtta tacatataga gcaattagtg
     4681 aacttattga aaacattttg ctccattctc tttttcagcc tgtaattaac ctcatgatat
     4741 gattaatatt tttacctaat gggcaactta aacccttata aatttacaat taacttattt
     4801 gttaatataa tcaccatctt ttgtaataac caataatggt gatttaacaa gaagaaagga
     4861 gcagtatgaa tacattattt actctaatct caactgtact actaacaagt aatatcacat
     4921 ccagcactcc attatttgcc aagcctggaa cagacattaa cacccaggta caacatctta
     4981 gccacaaggc ccctcaatta aacaagaaag tattaaagct tgctttaacg gcctataaaa
     5041 atgcgagtaa gagaggagca gttaaaaagc ctgtcctcac agtaattgat tattccttgc
     5101 cttcaaataa gcaacgtatg tgggtattcg atcttcgtaa tgaacgtcta ttatacaata
     5161 cgtacgtagc ccatggtaaa aattcgggag ttaatgttgc acggagtttt tctaacagag
     5221 agtccagcaa acaatccagc ttaggtacct atatcacaaa aaacacctat atcggacaca
     5281 aaggctactc cttgaacctc caaggcctgg acagaggctt taatgataat gcatacaata
     5341 gacgtgttgt gatacatggc gcgtggtatg ttgaacctga atttattaaa aaggcaggtc
     5401 gcgctggttt atcctggggc tgcccagcta tagcacaaac cttggcaaaa ccggtgataa
     5461 ataccattaa aaatggttcg gttgtttttg cttattaccc tgataaaaag tttttatctc
     5521 actcaggtta tcttgtagca taaaaaagta agccagggac tcagccctgg cttttcgcag
     5581 tcatccgtat aagaacttgc gttcttattg tcatcctgac aaatcagatc catctaattg
     5641 caaatccatt tgcacgactc atatccatat aagtcttgat taaaatttag caattgaata
     5701 ggggaagcgc aaggtgttaa atcacacctt cactcaatac tattttcaga tcagaaatag
     5761 tcttatatat ttattttatt aataatcaat aacttaccta cttgtgtaag aattattgcc
     5821 aggtaaacag aataatttct tctatggaat tgagaaattt cttacatcaa ttacagctta
     5881 aattacgtca ttttatcttt tagagcaata tgctgcttga ttatgtcata agatatggta
     5941 gtttatttac gatgaaatgt agcggggtat gattatgaac caacaactaa atgcaaccaa
     6001 gaaagataag cttcaagtaa tcgattggct tattgaaaat tttcctaatg cattttttaa
     6061 aaaaggaaac caggtcaaac cgctcaaaat tggaatattt gatgatctaa ttgattttta
     6121 tgagcgactg gatactccgc cttttagtaa aaaatcgtta agagaagcat taagctatta
     6181 cagtgcttct ccagcttact taagctgtca aaaacctgat acagcacgtg tagatattta
     6241 tggtaatgaa gtcgatgtag ttacgccaga gcaggccaag tatgcttatc agcgttatca
     6301 ggagcgctat ggcaataaaa aaagccaaga gttaaaatag cgcggaaaag ccacatttgg
     6361 atttgtttta ttgtaggacc ataatgtaat taatgcttac atcagaaacc agagtggtct
     6421 tcctaaggaa aggattatag cataggcctt tcatatcaat gagatttaaa tcgtaaaggc
     6481 gtgccatttt aactaactca ctgggtttaa tgaatttatc ataatcgtgt gtttgcctgg
     6541 gtaacaaatt aagcacatac tccgcagcaa taatagcgcc gagataagct tttaacgttc
     6601 tgctaatagt tgataaaaat aatagcccat ttggttttaa taaacgcctg caatgttgta
     6661 aaaccaactc agggttttgt acgtgttcca ataactccat acaagttatt acatcaaaac
     6721 cttgctcctc atactcttca ataggattgc aaaaataatc aatctgcaac tggtttttat
     6781 gagcatgttc tttggcaatt tgaatagctt ctggttctgc atctaatcca ctcacataag
     6841 ctcctgcctt ggccatagat tcacagagta tcccgccacc gcaaccgaca tccaacaccc
     6901 tcagcccctt tagattaatg tgctctgaaa taaactcaaa acgagcgcca ttgatatcat
     6961 gcaaagttct gagaggacca ttagtatccc accaatcatt tgcaagttgg gcaaatttat
     7021 gtacttcttc aatagcaata gtcgattcaa tttgattcat aagacaataa atccattggt
     7081 ttaaagaatt ctataacacg ttgattagta ataaagctta cattatatgg gtgttctgaa
     7141 accagtccta tcaataattt atttgcgggt attttctcag caattttacc aaataagatt
     7201 aattgcacat caggcttcct gatagccaat tgctcaaata aattttgcat aaatggcttc
     7261 caatgacgcg cgtgatacgg aaccttacct ttactgtata tcagggaagc attcaataat
     7321 aaaattccct ttttcatcat tcctttaaaa aaatcttccg cagtttgtac cagacatgat
     7381 ttatttatct tggcgattgc ttcttgcgag gtatttccat gcaaatcacc ccgagcgatc
     7441 aataacgttt taatccagtt gcgtagtgag gttgctcgat taacttcctt acttagccct
     7501 gtggagctcc acaataaacc tacagagtta tcccaaaaag catacccatt agcagaaatt
     7561 ttgcgtggat atggggactc gcctaaaaga atatattgtg tctggctcaa aggcaggcta
     7621 aaagcagaaa acagttgatt tagcccaggt aaccaatggc tatcctcttg tatttgtttt
     7681 aagtaatcac tatccattgt attcaatgct ttcatcagaa tctctttcca ttcctcatgg
     7741 catccaacca ataaattctc atacatctta atttttaccc ttccgtcatg ataattaaat
     7801 tttcaaattg aggtattttg ataattacct atgtttttaa accaggtttt cagccacttt
     7861 aacactattc ttagaccatg agaaattgtc ttattttaac atctggaaat aaaccccacg
     7921 ttggccaata gcttatctgt tgattgcagc gattttcttg ccatatcaat cgtggtgtgt
     7981 tgcctgaata ttcaaattac actaacatca tttaaaaatg ataatctgga gtgattatgt
     8041 ttttggccaa ctgcaatatc gaggaattag ttacagaaca tataaaacaa tttctagcgg
     8101 atgaagagtt gtctttctct ggcttaaaag atttaattct gagtaaagcc ccaattccct
     8161 ggattcactc ctctgtaaca gccactctgc ttaaatccag agactccgat aaaacagaag
     8221 taaaaaaaaa ccttgaacaa caatcttata aagctcaact ggctgaagac aagattcaaa
     8281 aagagcagga tgatgctgaa gcattaaaag ataaaaaatt aaaggaagtt ttaactcgtg
     8341 aattaaacca catcccaact caaatcagtg aacagcaaac ggaactcaga ttacttcatt
     8401 ataaactaga gcgactcttt gaaagccaag ctaaagtcga tgtgattcaa catcctgatt
     8461 ctcctatgaa aaagataaag ccgtcctcca gtcactctgc gtcgattgag cgcctgcaaa
     8521 gatctattaa tgagcgtgag attaaaattc aatcactttt tgaacaggaa gttaacaaca
     8581 agataaaatt aaacgaaatt gaaaagcgtg ccagtgtacg ctcacaacat cacactaaaa
     8641 gggttaaacg cgctcaggca agaattggat acaatagtac tggagaggat attctcagta
     8701 cattatcagg gaaaaatcaa tccattttgt tgcgcagtat tcaaaaacaa catcatgctc
     8761 ttgaaaaaaa atgcagtgat ctcattcagg aagctgatca gattaattac ccattatttc
     8821 ttgaggaact tcaaaagtat ttaaataaaa aaaagcatac tttatcttct caagaggtcg
     8881 atgcattaaa gtctgttata aaatatataa agcaacactt ggaatttgag catgaggtaa
     8941 tcaatacaca atccagtctc catattaaaa agcaatccat cagttcccag attgtaaaat
     9001 tacgagaatt acaaaataaa ctaaaaacct taaaaaacaa taaccccaat ttgacagcgg
     9061 caaatgaaga attagtttca cgtaatttgg aattaacggc catgaaagag catcatgctg
     9121 acctacgtca tcgcttgggc acacccgcat tacttttatt tggattaact tttctatttt
     9181 caattcctct tattttaacc ataagcggag taattccttt tttcattgcg cctgcgttgc
     9241 tttatatttt ggtctcagcg cctccaacca tcttgctgct atcaactctt ggtgttggca
     9301 tagcagctat cgttttcagc ttcaaaatgc attccaatga atcagctatt aaatccaatt
     9361 tgcaaacgat tgaaacaaac tctaatcaaa tgagccgaaa ttcccaaaat ttaaaatcat
     9421 tggaaacgct taccatcccc actctggata tgcaaatcaa aaaagatgaa aatttaagag
     9481 atcaattaat gctttcttta caaaaacagc aacgtcaagc tgcccaggca ttccagaaag
     9541 caaaagaggt agagtgccta tcctatgcta cctcttcact tttaaattcc ggcaataccc
     9601 aatccagtga tccatcatta tccacgcatt cagaagagag cgacgaaggg ctaaatgact
     9661 cgaatgactc tctgcaagcg ctggaagaag aaacagttaa tgagatagcg taaattgcct
     9721 ttctgtcaat aaaatttcaa tggagcaaga ctgagtatta accccctgta ataaaactgc
     9781 tagtcccaat attgataaat tagtctttat gatctaaaat gcaacaactt gattattggg
     9841 agtaatctat gtcacttgct acttatgatg taaaacagga actcgtcaaa cttacccaga
     9901 atttcttctc tattgatcat caattttctg aattaaaaaa cttattaacc gataaaaaaa
     9961 ccattccatg gattcataat caaatagttg atcagttatc tcaaattaaa aaatcagacg
    10021 aaatagctat gattgcgcgg ttagaaaaaa gcgcttacga acaacaaatg aaagaagatg
    10081 acgatgaaaa aaatcgggat gttgaagagt tacgccaaga tcttctccga aggaatgatt
    10141 tgacaagaca actcgaaatc ttgcgagtcc gcaaagagca atatgaaaga gagttgctca
    10201 tccgcaatac agtaccccat gtccatactc atccggacac tccggtaatt catcaacatc
    10261 cttctcaacc aatatctgca cccttgctaa accctaataa tcatgtacac actcatcttg
    10321 aagccacttt aagtagccag gatattgagc tgagtgttcg gaagattaac agaagaattt
    10381 cagagataaa taaagagttg gaaattctcg actccagaaa taatgcaaga gaaattcgca
    10441 gaggcgatag agaaaaacgc cttcaagcac gccttaatta tgttcaaaaa acagcaggag
    10501 ttgtcaacac cctatctcct gacaatcaga aaaaactgtt atccaatatt gagaaagaaa
    10561 aaaaatcact cacacaacaa cattcctcct tgcttctgaa agcagatcaa ttaaattact
    10621 ctatttttct tcagcaattt gagctctctt tacaatctat gcaacgtcct tttcaagaag
    10681 cagacgcatt gaaagctatt gtaaaaagaa tgaaagagca tttgaattac aaagaaaaag
    10741 cggcatcgat tcaatcccga ttagataata cagtgcttac tattggtgaa aatttaagaa
    10801 gattaacgcg acttaatact caattgagtt ccttgcagct tgctaatcct gatttaacaa
    10861 ggaggaatga gcgcctggag gaacaaaaca gagagctcct tgaatcgtat aatagccata
    10921 taaaaacacg aaataaactg ttttttccaa ctctcgttat aacaggatta tccttgctct
    10981 tttctattcc attgatatta actctaactg gaatcatccc ctatgctatt gcaccagctg
    11041 tattgttaac tttggttatt gctccccctg cattgttatt gcttgctgga cttggtgtcg
    11101 gtattgccac aatcatttat gctgtgaaag cttacttcaa taattcaacg attgaatcaa
    11161 accaggaaac aatagagagt aataggcggc aaatgggagc aaaccaaaag gaaatttaca
    11221 ctctggaaaa tcaaacaatt cccaatctta aaaaagaatt gttagagaac gaagaaatta
    11281 aaaatcgctt aactgatgaa ttacagtata tcgaaaacct ggccgagcaa gcattaaaac
    11341 aagcaaacga agtagagcca tatgtctact cagctatgcc tttctttaac cccgaggttg
    11401 ttgttcatca acatccttct gtaggtaatg gtatagtaac tcctagcgca cccccgatcg
    11461 atcccctcta tccagccgtc cggcattaat ttccagcctg ggttattaac acaggctatc
    11521 atccgggatg acttaagttg atttcccgga tgacattata gctatcagtt tctctttaaa
    11581 taattaccac aggcaattca gaccaggaag ttgtatagcg aggagaacgg gactgcgccc
    11641 gcatagccca tggcttcaca tgcccttctg ctgccaaacg caaggtatgg cggccatatt
    11701 tcgtgttgat ttcatctaat accgacatca atcgctctgt ctgattcaat tctttctctg
    11761 aaacctggtt aaacaaatct aactgcaaac agtttttttc aattaaatct cctaaataaa
    11821 cgcctacctt ttggtaatgg aatccagagc gaaatatttt acgcaggcac aatttggcac
    11881 aacgaatcaa atgtcgcaga tcatcagttg gattaatcag cttaaaccct atagtttgct
    11941 gatattgtgg caaatccaaa cgaaattgat tggttttgat aaatatggaa agatggcatg
    12001 ccatcaattt ttgatgacgt aacttttccc aagctctggc acaatggctg ctgatggcct
    12061 gagcaagagc agaatactct gtttgcatag aaccaaagga tttggaagat attatgcttt
    12121 ttcttgactc aaaatcctgt aaactgccac atgaaacccc acgcaactca agcactgttc
    12181 gttgcaatac aacactaaat aattttttaa ttaattgcaa atccatacca gccaagtcat
    12241 gagctgtata aatcccctgt tgtactaact ttttacccca ttgccgtcct atcccccata
    12301 cctcgccaac aactatttta gctagccaat gatattgatt ttcaatgtta aatacagggg
    12361 ttcttaattc ctttttcgca atatggtttg cgagtttagc cagggttttg gttgccccta
    12421 tgcctatgga tacgggaata ccggtatggc gcaaaacaat tttttgtaac ttgcggcaaa
    12481 actcatcatg ttcagattgg ggcaaagtat tcaaatcaag aaaagcttca tcaatggagt
    12541 aaatctctat tgctggccaa ttgtcttcaa gaactgacat aacccggcgt gacatatcag
    12601 catacaatgt gtaattggat gaaaacacat gcactttatg ctgcttacat aatgctttga
    12661 ctgcaaaata aggcactccc atttgaacac ccaaagcttt tacttcatta gatcgcgcaa
    12721 tcacacaacc atcattatta gacaagacca aaatgggttt attcgctaag gctggtttaa
    12781 ataaacgctc acacgagaca taaaaattat tacaatcgac cagcgcaaac atcacaatac
    12841 cgtatgaata acatgagtga cgactcccca aatcaccata tcgtattcct ctgtaatatc
    12901 tatgggagga aaatcagggt tttcagccag caacttaact tgcccatgtt gacgggataa
    12961 acgttttact gtcaattcgc cattcagggc aacgattaca atttttccat gagtagcctc
    13021 tatgccacga tcaacgatta aaatatcacc ggagtaaata ccggcattta tcatggattc
    13081 tccagaagct cgtacaaaaa atgtggatgc aggatgcttg atcaggtact cattcaaatc
    13141 cagcatggat tcaatatagt catcacccgg agaaggaaaa ccagctctta cagaactggt
    13201 atataaagga atcgctggga actccttttc tccttgaaga tatttcatca catcctgtat
    13261 acgagattca ggtatacgga ctgatttggt tgattctcca tatttacctt gcccacgagg
    13321 gcgcccagcg cctgctcgtg caccaccatg agacatatac tcccctaaac ttgaatttag
    13381 aatcaaaatt caagtttaat tttaaagtag cccattgtaa agttccgaca ataatgagag
    13441 tttcatatga cgactaaact gaattgacgc caatactcaa attacgttat tgttggggat
    13501 ttagtgtaag actaaagagc catggttaaa ttatataagg aaataatcaa tttaatagat
    13561 aatcaccagg caactatggt ggagcaactc catcaatttt gtgaaatcaa ttctggaaca
    13621 actaacttga caggattggc catcatggct gaaacgttgt gttcagcttt caaaccgatc
    13681 gctgatacca cagaattaat aacattaccg cctttgtctg tgattgacat gactggaaac
    13741 acagttatgc aatcctgtgg ggatgtattg ttcattagca aacgtcctca tttaaaacgc
    13801 cgaattctgc taagtgggca tatggatacg gtttattccg ccagcaaccc ttttcaaaaa
    13861 ttaagttacc tggatgctaa tcagatcaat ggcccggggg ttgctgatat gaaaggcggg
    13921 ctaatcgtca tcttgcacgc attagcggcc tttgaaaaca ccagatttgc ggaagatatg
    13981 ggttgggatg tgttaattaa ttcagatgaa gaaattggtt cacctacatc aggttcctta
    14041 ttcaataaat tggcacatcg ctatcaggcc gctttggttt atgaaccagc aatgacacct
    14101 aatggtactt tggcaaaaaa ccgcaaaggc agtggaaaac taacactggt agcaacagga
    14161 agagctgccc atgctggccg ggcatttgat gaaggaagga acgccatatg ctatcttgct
    14221 gaagcaatta cggctgttca tgccttaaat gggcaaaaaa atggagtaac aatcaatgta
    14281 ggcaaaatag ctggcggaga agcgctgaat gttgttcctg ataaggcagt agctcagttg
    14341 gatatccgaa ttagtctgcc agaagatgaa atttgggtaa gaaatgaatt aaataaaatc
    14401 atcaaacaat tagagcgacc tgattattcc ctaaatgtgc atggcacctt tggacggcct
    14461 gtgaagcgta tctgcgcagg gacagaacga ctttttcatc ggattcaaaa aattggagag
    14521 gcgcttggct taactattga ctggaaagac agtggaggtt gttgcgacgg taataatttg
    14581 gctcactatg gcttgcctgt tctggatact ctaggtgttc ggggaggcaa tatacacagt
    14641 tcagatgaat atatattgct tgatagctta tcagagcgcg ccgcacttag cgcgcttctt
    14701 ctgatagatt tggcacaggg tggtttagag gatctaataa aatgatgtta tttcgtagcg
    14761 ctcgcgactc tgacttggat gcgatccatc aactggcaga aatcagtggt gttgggatga
    14821 caacgctccc taaagataaa gaattattaa aaaaacgctt ggaatggtct tgcgaatcct
    14881 atcaaagaaa tgtcactaac cctggctgtg aatattattt ttttgtcctg gaagaacaaa
    14941 aaaataatac aattgtcggt acctccgcta tagaagcaag aacaggttat gaaatacctt
    15001 tttattctta taaactctct aaaagaacca gagtctgtca cacactcaac atacgcagtg
    15061 attatgaagt attaagttta gtcaatgaca atcaaggtcg cacagaaata tgtactctgt
    15121 ttcttcatcc tgattacaga aaaaataaca atggcctatt gctttcgaaa ggacgctttt
    15181 tatttatggc acaatatcct catcgcttcg ccccgattgt catcgccgaa atgcgtggga
    15241 tctctgacga taaaggcaac tcgccattct gggataatgt tggagctaat ttttttcata
    15301 tgccttttgt agaagcggat cgtctgacac tagcgacgaa caaacaattt atcgccgatt
    15361 taatgccccg caatcctatc tatgtcaaac tcctttcgcc agaggcgcaa gcagtcattg
    15421 gcaaaccaca tcaatcgact atccctgcca tgaatattct cttaaaagaa ggctttcgtt
    15481 ataacaacta cgttgatatt tttgatgccg ggcctactat cgaatcacca ttattagaaa
    15541 ttaaaaccat ttctttaagc cggatcatgg ttattaaaaa catcagtgat gaagcgagca
    15601 gtagcaatcg ctatttactc gccaataccc aaatggattt tcgagcaaca attagccacg
    15661 ctgtttttaa tgaacgagaa aacacctgca ttattagcaa ggaaacagca aaattgcttg
    15721 atgtaaaaca aggagacagt ctacgtatat cacccatacg tacagaagat actccaccat
    15781 tgtaaccaag gaataatgat gtctaaatta caaatcatac agagtaaagg ccaatatatt
    15841 aatggagaat ggataaaggg aaatggtctc attctggagt ctacaaaccc cgcctccgga
    15901 actttgcttt ggcaagggaa taatgctaca gatgaagaaa ttgcaaacgc ttgttacgtt
    15961 gcgcatcgcg cactgaaatc ctgggctaat accagttttg aggaaagggc tcgttacact
    16021 aaggcttttg tggaacaagt agaaaaaaat cgtgagcaac tggcacgact tatttcatta
    16081 gaaacaggaa agccattatg ggaatctcaa actgaagtca gctcagtaat tggcaaagta
    16141 aatttgtcaa ttcaggctta tcaagaaaga acctggccaa aacaaactga aactgcagaa
    16201 gctaatgcct gcctccgttt taaaccgcat ggtattgttg tagtattagg cgcgtttaat
    16261 ttccctgccc atttaagcaa tggacacatt gtccctgccc tccttgcagg gaacacggta
    16321 ttgtataaac caagtgaaca tactccagca gtagcggaat tgatcatcca atgttggcac
    16381 gatagcggct tacctcccgg agttatcaat tgtctacagg gtaatgcaaa ttgtggaaat
    16441 accctattgt ctcaagatat tcaaggcgtc tattttaccg ggagttatgc aacagggttg
    16501 cgtatacacc aacaattctg taatagaccc gaagttattt tagctttgga aatgggcgga
    16561 aataaccctt tagtcatcga tgaagtgaaa gacattgatg cagctgtata ccatacaatc
    16621 ctttcaacta tgatcactgc aggacaacgt tgcacatgtg cgagacgaat tataattcct
    16681 gactcccaga caggcgatct atttttagaa cgctttgcca aggcttgtaa attaatgcga
    16741 attggatcct ttgatagtca gccagagcct tttattgggc ctgttattag ccatgttcaa
    16801 gcattaaaac atctacacgc tcagaagcaa ctaatcgaaa tggggggtga aatcattctg
    16861 ccgatgtcgc ttcttcttga gtacacggga cttgtttctc ctggcattat tgatatgact
    16921 cgcgccaaga acccgccaga tgaagagatt tttgcgccat ttgcacaaat ttaccgttat
    16981 aatcactttg atgaggcaat tcaattagcg aatcagacac gttatggttt gtcagcaggc
    17041 ttactcagcg ataataaaga ccattatcag cagttttatc aaaatattcg tgccggacta
    17101 atcaattgga acagaccaac cacgggtgct gccagtagcc ttccatttgg tggtgtcgga
    17161 tgcagtggaa atcatagacc aagcgcctat tttgctgctg attattgtgc ttatcctgta
    17221 gcaagcatgg agcaacctct gttaacaact ccagtgcaac gattgccagg tctggtattg
    17281 gagtaattat tatgaatgtt tatgaattga atatggatgg gcttgttggt caaactcatc
    17341 attacgcagg gctctcctcg ggtaatatag cttctacaaa taatgcttta tctatttcca
    17401 accctcaagc tgcagcaagg caaggtttgg aaaaaatgcg gcaactttat aacatggggt
    17461 taaagcaagg cctattgccc ccacatcaac gacctaattt gaatttactg tatcaactgg
    17521 gatttaaagg cactcccagt gagcagataa acaaagccta taaaacagcg cccgaattgc
    17581 ttagtgcctg ctactctgcc tcaagtatgt ggacagctaa tgcagcaact gtttctgcaa
    17641 gcgtggatac tgaagacaac aaagtacatt tcactgctgc caatttaatc agcaatttgc
    17701 atcgtcacca ggaagctgat ttttcaaaaa aattattgga atttatcttt tccaattctg
    17761 attattttaa tcatcaccct ctgttaccca aatcaatggg cacaagcgat gagggagcag
    17821 ctaatcataa tcgcttatgt caaagtcatg cgcactcagg gattaatctc tttgtatacg
    17881 gtaaaaaagt gctgggaaat catcaattcg aacagagccc aataaaatac cctgctcggc
    17941 aaactaaaga agcgtcagaa gccatcgctc gcaaccattt attaaatcct gaacgagtaa
    18001 tctttgcctg tcaaaatcca ttagcaatag atcaaggtgt atttcataat gatgtcatct
    18061 cagttgctaa tgaacatgta tttctggttc atgaagaagc tttttataat caggcttatg
    18121 ttttggatca attaagagaa aaagccgatt ttcctttagt aataattcag atctcaaaag
    18181 aacaaatcag cgttagtgaa gcagttgata cctatctttt caattctcaa ttgattaccc
    18241 tgcctgatca gaagaatatg attttaatag cccctgctga atgtcaggca aaccttaaag
    18301 taaaaacttg tattgatgga ttggtcgccg atcctcaaaa ccctattaac tcagtgtatt
    18361 acctggatct gaaacaaagc atgcgtaatg gcggaggacc agcctgttta cgcttgcgag
    18421 ttcctttgaa tgactacgag cttaaagcaa tgcaccaagg tattctaatt gataatgatt
    18481 tgttagatat tttagataaa tgggttttaa aatattaccg aactgaactg aaaatccccg
    18541 atttagctga tccgcaacta ttatatgaat gtctggatgc attggatgaa ttaactcaga
    18601 tcttgaaatt aggctcaata tatccctttc aatcttgagt atccgagtca tgtggaaaac
    18661 aaaattcggc aaatgtatct atatttcacc gtcaggatat aaagtctatc aaaatttgtt
    18721 gtaccgttgg cttactttag gtagcgaagc atggcaaact gtaattaacc gttatatacc
    18781 tcgcaggcct ggtttacatt atctgcaaat tctgacactt atggcgcgaa aatttcctaa
    18841 tgattgttgt ttattaggac ttggaggagc aggaatcgct cacctgctgg gtgcacaaaa
    18901 aaccatacgc tctatcgttg ccgtggaaag tagtgaagaa gtgattcaaa ttgccaaaaa
    18961 atttttcatg acagatttaa ttcctcattt caccattact caccaaaacg cccgagattt
    19021 tgtacgtgaa aacaaaaaga cttttcagca catgcttctt gatctatatg acgcaacaca
    19081 ttttcccgat gagtgtcatt ctatggattt cttcattcat tgtaaaaaac aattaactga
    19141 cgatggattt ttagctgtta acttagcaaa ttcccaagaa caatgggctc tggtccaact
    19201 aatcaagaaa caatttccat ccaatatcat tattccaata aaaaaatgtg ccaatattgt
    19261 tgtcattgcc tccaaaaatg acaacaaaga cttgtttatt aaacaagtca ggaatactaa
    19321 tgaaataaaa aaaattacat gggtaaaaga ttggggttat gttgctgaat attaatgttc
    19381 attgaaagga aacaatagtt tatttacaaa tgaaacccag ccaaaagctg gtatgtattt
    19441 aaatatttta tcttattttc gcatgtaatt aatcgcttca attaatgcct catcagagca
    19501 atcggtatta gggacatctg cctctttaca taaaccgcca tgatgcatca accctttacc
    19561 caccttgaca ttgtacagca ggtaatccat tgcgctttca aattgtgaag gattattttt
    19621 tgcttctatt tttgcttgtt taaatcgcag cttccatgcc tttttatcaa aagcaggagg
    19681 ggcttttata gccttggcta aattaggggc atgacaattt ttacaggcaa tttcgtaagt
    19741 atattttcct aaatttttag caaaaactgg agtacttgtt aaataaacta acattataat
    19801 caatgcattc cttttcatca catccccaaa ttattattgc taaagcaatc ttaggattat
    19861 atgagatatt ttgccaattg aaatagtttt ttacttttta attaaagcgt atagaccatt
    19921 atcttgatgc ttaattatag aaatacccta accaatttat tggtactgaa gagatatgaa
    19981 caaaaatgaa acaaatatgc taaaagtcaa ggcaagatca ttcctatttt agtcgattat
    20041 gcccgtgata tactttataa atttgagcat atttttgtca ttctcgcacg gtcgggaatg
    20101 acaaaaattt agcattatat aattagagta tgttttttct tgtactaaaa acaagaacta
    20161 tagctctatt atataaaagg agtaacatct ataccttaaa tttccattaa atctttttct
    20221 ttctcggcta atactgcatc aacttcagaa atatatctat cagtcagctt ttggatcgct
    20281 tctgctgcac gtcgttcatc gtcttcagaa attgcttttt ctttaacaag ttcctttagc
    20341 tgattattgg catcccttct gatatttcga attgaaactc ttccttgttc cccttcatgg
    20401 cgaacaactt taatcaattc ctttctgcgt tcctctgtta aaggtggcat cggaactcga
    20461 atggctgttc cagcagttgc aggattcaaa cccaaatcag agtttagtat agccttttca
    20521 atagctgcga ccatagattt ttcccagggt gtaactaaaa tagttcggga gtcactggta
    20581 gtgatgttgg caacctggct taaaggtgta ggacttccat aataatcaac catgacatga
    20641 tctaaaagac tggcattcgc cctgccggta cggatttttg acatatccgt atgcaaagcc
    20701 tcaatagtct ttttcatgcg tttttctgaa tcttgcttaa tctcattgat catgattagc
    20761 tccgactata gttcctactc gttctccagt tacaattcgt tttaatgctt taggagccgc
    20821 catatcaaac acctgtaaag gcattccctg atcttggcac aaacaaattg cagtggaatc
    20881 catcacttcc aaacctttgg ttaaaacttc tttgtaagtc aaataatcat aacgctttgc
    20941 atcagagttt ttaagtgggt cagcagaata aactccatcc accttagttg cttttaaaac
    21001 aatatctgcg cctatctcaa ttgccctcaa acaagcagca gtatctgttg taaaaaaggg
    21061 atttcctgtt ccagctgcaa aaatcaccac ctgaccattg cgcaagtgag tgatagcttt
    21121 tcttctatgg tatgggtcaa ctaccccaag cattggaata gctgacataa tacgagcagg
    21181 cagatcaatt cgctccaaag catcacggag tgccaaagca ttcataacag tggccagcat
    21241 ccccatatgg tctcctgtca ctcgtcctac gcccgcctga gagagtgctt tccctctgaa
    21301 cagattacct ccacctaaaa ccaaaccaac ttccacaccc atatgaatta attctgctat
    21361 gtctcgagct aatgagtcaa gaactgaagg atcaatccca aattgagatt tccccatcag
    21421 cgcctcacca ctaaatttaa gcagaatgcg tttgtatttt aatttaggtt gactttcatt
    21481 catcatgtac gaacctgagc catcacttct tcaacaaaat tgtcctcttt tttctctatt
    21541 ccttccccta cttcataacg aacaaaggat attacttcag catttttctc ttttagaagt
    21601 tgacccactt taatgttagg atcttttaca taaggttgtc ctaacaagct tacctcatca
    21661 ataaatttat tgattcgccc atcaatcatc ttgtcaatga tctcttgcgg cttaccactc
    21721 tcttttgctt gagcagtgaa tatttctctt tcgttttcaa tcgcttcggc tggtacctga
    21781 tcacgactaa ccaccatcgg tttgctggca gcaacatgca tggcaatatc tttagccaat
    21841 gcttcacttc cattctttaa tgcgaccata actcctatac gcgaaccatg caaataatac
    21901 ccgattacac cgtcgcaatg cattttttcc aatcggcgta atttaatatt ttcaccaatt
    21961 ttagcaacca gctcctgtct tgcttgctca acagtagcac cagaagataa tgcctgattg
    22021 gacaagtctt ctatgttttt tggaaggctg gttaatgcaa catcggcaac agcatttgca
    22081 aaattagtga aattctcatc tcgtgcaaca aagtctgttt cactattaat ctcaagcatt
    22141 accgctgtac gttcatcaga agaacgtgca ataacaatta taccttctgc tgcaactcta
    22201 tctgcctttt tgtctgcttt tgcctggcca gctttacgca tttcaatgat tgcttgctcg
    22261 atatcaccgt tagtagctat tagaaatttt ttacattcca tcatacctgc gcctgtgcgc
    22321 tcacgcaatt gcattactaa tgcagcacta attgttgaca ttttgatttc tccacatagc
    22381 aaaaaggggg cttattaaat taacgccccc tttattcaaa tagtaattat tctccggatt
    22441 tttcttcttc attagaattt gaagtaacct caacaaactc tgaatcagat gatactcttc
    22501 ctacagtatt gctattttta ccatctaaaa tagcatcagc gacacagcga acataaatgt
    22561 ccacagcacg catagaatca tcattgcctg gtataacata atcaatgttg tcagggctat
    22621 tattggtatc aacaacacca attaccggaa ttcttaatcg acgcgcttct tcaacagcaa
    22681 tatgttcaaa accaacatca accacaaata acgcatcagg taggccgccc atgttctcga
    22741 ttccaccaag gctacgctcc agtttttcaa gctctcttgt cagcattagc gcttctttct
    22801 taatcatgtc attaaacaat cccttttcct tcatttcttt taattctttt aaacggaata
    22861 ttgattgacg gactgtttta tagttggtca gcatacctcc taaccatcta tggtctacat
    22921 aaggcatccc gcatcgtttt gcatgctctc gaatgctgtc ttgagctgct cttttagtgc
    22981 ctacaaaaag aatttttgcc ttgtttgctg ccaatcgact aacgtaattc gttacgtcac
    23041 tcagcatggg taatgttttt tcaaggttaa tgatatggat tttatttctt gagccaaaaa
    23101 tgtattcact catttttgga ttccagaaac gtgttctgtg cccaaaatga gcacctgctt
    23161 cgagcaattc acgcatacta acattattca tatatatatc tccagggtta agcctccacg
    23221 cttcccatac gaaacccacc aagggaccct atcgcatgtg acgaaacgtg tgtgtattta
    23281 aagcgcggta tttataacat attcatgcca attcagcaat caaattaaca ttgctctctg
    23341 tacatgacaa aaatatgtca tgtacaaatc attatgtatg accacagtaa ttagacaaag
    23401 cctattagga aataagtcta tattaatttg tacattttga tttatactgc tatcagcatg
    23461 gaagttttaa aaatgttgta ctcaggagaa tcctattaac gataacattt aagaggaaac
    23521 gataatgagt aaaaaatcta tctgtttagc cacgctcgcc ttgatattta cctttggtca
    23581 gcctgtcttt gccaactcaa gcgaagctac acaaacaaag ccttgtcatt gccatgatca
    23641 caaaaaaata ctcagtcagc ttaatttaac tcaagagcaa aaaaccaaaa tcaaaacaat
    23701 caaaatacaa gcccaaaaat caatcaaaga aaattataga caacttaaag cgatcaaaga
    23761 acaaatcaat gcgctcattg caaatgagaa gcttgatgaa gccaagctgg atagtttaat
    23821 caaccaaaga aataaaataa cagggactat gctaaaaaat cgtgtaatga tgaaaaatca
    23881 aatttacaat cagctaacga acgaacaaaa atcacaatac aaaaagaata tgtaaaacct
    23941 gattggtacc gataataaaa aattcggtac caatctatcc caactcttca gatataatca
    24001 ttgctgtaat tatgatcttt ctcactgttg cttcctgaac aatattttct gaacgcaaaa
    24061 aaatgggcaa accttctctc tctaccatta aaaaacagat gatatttcgc tcagtatgct
    24121 aacccatagc atgctgcagc agctgcactg gcaatgatgc aaagtgcaaa tattatagtt
    24181 ctcgccatat tcttctttgt tttgggtttc ataacaaatc ctggaatgac ggttatcctg
    24241 attgatattt catttaaatt atagttcaaa ttttatgcac atccagaccc acatattgat
    24301 tgcccttaca ctgagtagca tggagcttta ctacaaattc attgcaaaac acttgctaca
    24361 attattgact tatgacccaa tttagaacaa cctatatcgg cgatgagcaa tattgttgtt
    24421 gcagaataaa aaagaaaccc aattatgaga ggacatatga tttttttaag tattcctaaa
    24481 gggatggaat ttaagcaaat tacagaaaaa gacaatacga acgactattt tgttgatccg
    24541 aatggaaagt taccacgaat taatattcag gctttagtta aagacgctct gcagtacaac
    24601 aaaggtagaa aaaaagaaat ctccctgcct gacttcacta tttataggca caaaccacct
    24661 tatagagatg aactcttcct tcagtataat ccagatcata atggtaaata ctttacaaaa
    24721 gaatctgtaa atttggtgaa tggtaaagaa tttattaaat ataaaactcc tgctacttca
    24781 tacggcactt tttggtttca aaaagtacaa ttatccgaaa gcagaatgga tgaagtgctt
    24841 gccaagcgaa gtgaacaacg agaaaatcgc aggcatacag gggatagtcc aaacccaaca
    24901 taatcaaaca agaatagtga aaaggcatgt actagcatgc cttttttcaa aaagaacaaa
    24961 atgaaattaa atggccaatg cagtagaagc tttagtgtct aatttttgaa ttaactgact
    25021 ttgcatgcac aaatatgcgc ctacaacttt ataagactct tgtgaatgga aattttttgt
    25081 tgagtattct ttaggataag tgaggctttc cagccgatta tttgctgcgg ttatcacttt
    25141 gtctaaatca atcaaggtgg tagagtaatt atcaccactg tctgttatca ttttcatttc
    25201 ttcaagagcc tcccgtacaa cagtcagaat tttattaaaa tttagaccag cagtggtgca
    25261 acctcgatag atagtaatta ggcgatcgac aagagaaaag aaattaacca tcttttgcaa
    25321 atttgactca agaatacgca tacatgctac catttctgtt gtgctgcgtt gaggatattt
    25381 gtccattaaa gcgttcatca cttcgacaac ttttttcaca aacccatcta cagctttaac
    25441 ggaataactg tttttcttaa acacttcgaa aatagattga gcatttctca ttggatcctt
    25501 taaagcattc atacttttaa taaacgcatt aatctgctcc caaaagcggc tgccatcctt
    25561 gctgttttct ctagcttctt gatagttttt gattaacatt ttcgcagtaa acgcatattg
    25621 gggtaagaaa aagcgatcgc ttttctcagc cattatcgcc tgcaaagtaa aaattggatt
    25681 gttccattgt ccttcatcac acaggagatt ccattgctgg gctttcgctc tgattaaaaa
    25741 gactttttgt tcatttgata acgtagtgat aacgttgatt tttgctttat acaattcaaa
    25801 tgcaccaagc tttattttct ctatagcctg ttcatgagct ttaattaatt cagcgggaga
    25861 attaaaatta ttaacaaact tcattggttg taacttattg gcttcaaatt caaaattaca
    25921 attgtatttt gcctgacgaa ctcctgcagc attaatttct tttccatcta tgtcataaac
    25981 atctcttgca ttctcaatag ctttatcgcc aaacgctttg attgttttga catagtcagg
    26041 attagtaaag ccctttccct gaacaggaat tttgtaattg tccatggtat ggacattttc
    26101 acggctacag gcatatttaa ctaaatgcat tgtaacgtta gatggaataa gctctggatt
    26161 tgctgagaaa aattgataca aaccttcaag tatatcttct cgatcatcat aaaaaaaata
    26221 atcaatctgc tctctagcct ccaaggaaga agctctttgc atgtgtgcat acacaagcga
    26281 agctttcgat ttatcatgta accagccgtt ttgaattttt gcgccatttg caggattcgt
    26341 tgcctcatgg agactagtac ctgctggcac caaattgtat gcatcagcta ataaaaacgg
    26401 atcaaattcc atgcctaagt cggtgcacag tttttgaatc tggataaaac aagaaccatt
    26461 gccattggag cgagcattac gcttatcatc gcgatcactt tgccgattcg aagcaattag
    26521 cgcgacttct ttagtatagt agtttgaagt ttctcgaatt ctttgggcaa gctttggatt
    26581 ttgtgcaacg atatttatta gattggtaac accgacgcaa ccatcataat caaaacctga
    26641 ataacgaact ggcattttat tttcctctca aaaagattgc ataaccactg tcactttaat
    26701 taaggagaaa atttcaaaca tggaagtcgg aacatcgcct tgagtaaagt gatactttct
    26761 tcatagcgac gcacccgttt cctcatgaaa ctagaaatag ccagtcctct aggcaatcct
    26821 tatagagaag acacatcgaa attttgctaa acaatattaa gcaaagttca taatgttaac
    26881 attatgcaaa gatatgtgtc aatttcttat ttattgttgt ttttctattc atagttcaaa
    26941 cagccccaat caggttcaat ttggactgta taagctctca tataacacga gttaatagag
    27001 agctgggaat ttctgatata agatatgtaa acaggaaata tcaaatcaaa aaaacttgct
    27061 ttcaagtgtg atacttgaat ccataatacc taattgctct aacaagtctt caggcaaatg
    27121 caaaccgctc tccctgagct gcattaataa aatactacgc tcatttgagt taaaatcttt
    27181 tatcgctttt ctaaaagcat tgatattaaa ttgattatgc ccttcattat cactcatata
    27241 atttctttta aaataatccg gataaaaagc caataaaaca agagctttat caaataattt
    27301 attgttataa caatgcatga cgatatcgaa atcattctca tcgatgtatg taaagtattc
    27361 attgatatca ataggatttt gtcctattaa aaattttaca acagattgaa aatcaaggtt
    27421 attcagtact aatgcggcat tatgtaaaaa agtcttgtct gatcggtcat gcactaaaag
    27481 attaacctga tttttaatca atagctgaat gatatcaatg tatttttggg tatccttcgc
    27541 taaagaatat cctttcaatg tcaaactcaa aggacaatga ccagaatgac tttgctgttg
    27601 atcgatatta gcaccatgtt caataagcca ttttacattt tcaacttgtc caagataaca
    27661 ggcagccatc aaaggggtga atgtatccgc ttcatcaaaa tctaatattt tttcgtcaga
    27721 acccactttt aaaaatggca aatcgatatc tgaggcattt aattcttgtt cttttgcaag
    27781 agaatagtta gctaaaatta tccgtcctat ttgagtcatt aaaactctat cccctgcttt
    27841 aagatcaaat ccttccaaat tatagggcat ttgttctaat cggtactgag attgataagt
    27901 ttgataaaat tcttgtgccg atagccaacc atcgccagga ttaagaaagt aataatacct
    27961 ggaggcctgc tcttcgtagg tattatagtt gattaatagt tgttgtcctg ccttgatgtt
    28021 tttagtggtt ataacttttg ctactttctt gcgattcaat gtagtttcaa caaactctgc
    28081 attatcctga ctatccgaaa aattgatata acgagtcaaa ttaccttttc ttgctgcatc
    28141 aattacccgt cccccaacat acatcgcgta acttttatca gaaccaacat gttcctttag
    28201 atattgttca aattcttgtt cagaatatac ttctcctgtg taaataccaa tacaagtccc
    28261 ctttgggata tcttccctgg caaataaacc tcgtccgccg gtttttctga gtaaatggac
    28321 agactgaacc ataacaagat cagttctgtc tatagcagat ggaatgtaat cgctgcttga
    28381 catgttatca atatgtaaat caagcataat tttgctcgtg tcaatccatt catccttgct
    28441 tttcggtcca ttgacaggag taaacagcgt gtaaatatta cctgggaggt gtgtcatccc
    28501 aggtcctttt ttctttgcct ttctttggga aaatttaaat ttagatttgt gctctttact
    28561 accatgattc aattgctgtt ctttaaactt tgactgaagg gcacttttct ttttaagttt
    28621 gctatcattc ttgcttcttg gcataacact gctctcttgt aaaaaatcat ataaaataat
    28681 cattaaggag ccattatact ctattagagt attttttact ctattttaca tgtgattttc
    28741 attgaaaatc tattattttg ctgattgaag agtattgttg cccctcttac ccatgtgcta
    28801 acatttatga ttttggataa accataatgg gcgttacaat taaaactcct gatgaaattg
    28861 aaaaaatgcg tgtcgctggc agactggcag ccgaagtact tgagatgatt ggtccttatg
    28921 ttcaagttgg cattacaact gatgaattaa acactatttg tcacgattat attgttaatg
    28981 tacaaaaggc tattcccgcc cctttaaatt ataacggttt ccccaaatca atttgcacct
    29041 caattaatca tgtggtatgt catggaatac cagggaaaaa aactttaaaa gatggagaca
    29101 tcatcaatat tgatgtcact gttataaaaa atgaatacca cggtgatacc agtaaaatgt
    29161 ttattgtagg caccccttct gtcaaggcca agcatgtagt gcagatagca catgagtgct
    29221 tgtatattgg aattgatatg gttaaacccg gtgtccagct aggcgatata gggcatgcca
    29281 ttcagcaaca tgcagaaaaa aatcgttgct ctgtggtgcg cgattactgc ggacatggaa
    29341 ttggccgtgt atttcatgag gatccacaag tgcttcacta tggagttccc ggaacaggga
    29401 tgaaattaga accagggatg acttttacta tagagccgat ggttaatata gggaaacatc
    29461 atactcggct attaccagat cattggactg tcgttaccaa agatcatagt ctttcagctc
    29521 aatgggagca cacacttttg gtaactgata caggtgttga aattctgact ttacgtaatg
    29581 aagaacgata atcgcatcat aaaaaatacg attaaacagt tcaaggagaa gctgtgcaag
    29641 gactttagcc aaaaaactaa tattacctcc atcacacgca agcttgctgt atttattgat
    29701 actattttaa ttcaattatt tattaaaaat aagctacact ttggagataa tttttgtctc
    29761 ctggctcttg gaagctacgg gcgtagagag cttcaactcc attcagatat tgacttgtta
    29821 atattgcaca ctgaaaaagt aagcaatatc caattacaac gcgcacaaaa atttatccag
    29881 gattgttggg atgtggggct ggaggtaagc catcaaatta ccactgtttc ttcatgtgcc
    29941 aatttggcca gtcaggattt gagcgt
//
LOCUS       DACTJE010000020        29523 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.020, whole genome shotgun sequence.
ACCESSION   DACTJE010000020 DACTJE010000000
VERSION     DACTJE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 29523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29523
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.020"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            50..784
                     /locus_tag="I9760_000689"
     CDS             50..784
                     /locus_tag="I9760_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT5042477.1"
                     /translation="MRKVFSLLLISSIIVISSCSEQKNQNTLRFSTSAEYPPFEYIEN
                     GEIKGFDIDLAKLIAKELGKEAVFDNMQFSAVLPAVNSGQDDVAIATITITEERKKNF
                     DFSKPYYFEGMAAVFDTSQPVKEISQLTGKKIAVQLGSVMEIWLHQNFPKAEITALDN
                     NNQAIEALIAGHVDVVLMDGAQGGVYSKKYPGLSYSILAKANEGYGLVLKKGSHLRDE
                     INSALENLKAKGEIQKLESIWIKGSI"
     gene            781..1428
                     /locus_tag="I9760_000690"
     CDS             781..1428
                     /locus_tag="I9760_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAT5042478.1"
                     /translation="MTEIVQNLFFIGKGIVLTMQLLTGGMLIGVLLGTLLSICRHQGV
                     ACFVINRFISILRGTPLILQLSFIYFAVPVLLGMRPSILVAGIVAFGLNSSAYFAEIL
                     RAGIESLPKGQFEAALTLQIPTFYLWKDIILPQVIKNIFPAMINEMIALLKETALIST
                     IGGMDLMRQAQTLAAQQFTYFMPLCITGFYYYSLVLLLEALGKKIEKRGHYAASH"
     gene            1412..2080
                     /locus_tag="I9760_000691"
     CDS             1412..2080
                     /locus_tag="I9760_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAT5042479.1"
                     /translation="MLQVINASKKIGNAQVLNEINLSIQANSIVGLVGPSGSGKTTLL
                     RCIQQLESIDSGSIHCEGKSGFMFQDFQLFPHMTVLNNLIYAPYLHDKTADHMANAWA
                     LLAMLGLSDKAHSYPQGLSGGQKQRVALARSLMMKPDLLLCDEPTSGLDMATTNDVIA
                     LLNTVKTLKITMLIASHDLFFLSNIAERLLVVKQGQLIADFKTQEVSDPILYLQQFYQ
                     GEIL"
     gene            2077..3294
                     /locus_tag="I9760_000692"
     CDS             2077..3294
                     /locus_tag="I9760_000692"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAT5042480.1"
                     /translation="MKKVIKKIALAYSGGLDTSIMIPWLKEHYEHAEVIAVICDLGQQ
                     EDLDTIKNKALKSGASKAYVVDVKNEFAIQYLWPLVKSGALYEDQYILGTISRPLIAQ
                     KLVEIALTEEVNAVAHGATGKGNDQVRFEYSIKALAPQLEIIAPWRTWDIKSRQEAIV
                     YAKAHGIEVPVTPKAPYSRDHNIWYISHEGGVLEDPSQEMPDDVLLMTAPVSQTPDEE
                     EVVVLDFKKGVPVALNGQELSPVDLLNSLNQKAGQHGIGVADIVENRLVGMKIRGIYE
                     APAAAVLYKAHKLLESLCLTRSTLHLKQSLQQTYANLVYEGRWFSQTKQALDAFIDVT
                     QQHVTGCVKLKLFKGNIIPAGMHSPYSLHHPELATFEEDNVYNQKDAEGFINLFSLSA
                     KIYSQVHQEGNYD"
     gene            3287..4522
                     /gene="argH"
                     /locus_tag="I9760_000693"
     CDS             3287..4522
                     /gene="argH"
                     /locus_tag="I9760_000693"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAT5042481.1"
                     /translation="MTNKTWGGRFKKSLDSGVNQFNASLSFDHVLFDQDINGSQVHVK
                     QLAKQKILTEVESQAIYSALEEIRSEIKQGQYSFNVRDEEDIHMFIEQLLIQKIGDLG
                     KKLHTGRSRNDQVALDLRLYTRDKGCLINELLTRLIDCLDDLTSKHQQDLMPGYTHLQ
                     QAQPVTLGAYFNAYQCMFGRDKSRLEDWFKRMNYSPLGAGALAGSTLPLDREWVAESL
                     GFAGIIPNTLDAVSDRDFVIELCSVAAMIMMHLSRLCEDLILWSTQEFNFVILDDAFA
                     TGSSLMPNKKNPDVPELIRGKSGRVYGHLMAILTVMKGLPLAYNKDMQEDKEGLFDTI
                     NTIIACLQMITPFLQSLTFNTLLMKTKAQSGYLDATAILESLVMKGMPFRDAHHQVGA
                     WIAEAIEKQCSLNELLKGG"
     gene            4525..5640
                     /gene="argF"
                     /locus_tag="I9760_000694"
     CDS             4525..5640
                     /gene="argF"
                     /locus_tag="I9760_000694"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAT5042482.1"
                     /translation="MSFSTSTNLTPIETQDDDLEKIQSQNNPSAINEVKTNTFVINLQ
                     QAIFGLETGSEAVDFDSHKNTGFPFPAGEYKPRHFLTGTELAREEIQFILSQARSLKQ
                     NPGLYNQALANKSLVMIFEKPSFRTRLSFALAIQSMGGIVVESIANSRKQETPADMAR
                     VLNGYADFVMVRTHEDSFLQEMAEYSKIPIINGLSALHHPCQTLADLLSLEEQFGALE
                     GLTLTYIGDGNNILHSLMLLAPQLGVTVHYCCPKSNQPNEAIIKEAQSKTGKQMIYCF
                     DKPEEAVKQADAVYTDVWTSMGFDNPEAGKIFKGFQINETLMSHAKPDSVFMHCMPME
                     RGKEVSATLPDSASSIIFRQSENRMHVQKALMLFLDQ"
     gene            complement(5730..7205)
                     /locus_tag="I9760_000695"
     CDS             complement(5730..7205)
                     /locus_tag="I9760_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAT5042483.1"
                     /translation="MNITRLLAFFTSFFLLCPIYASVDSYFSHINKDSSALYAFFKTM
                     PKGGELHYHLAGGAYPETMLALAANGNYCLDKHSFAVSKDSSHCEGVNVKEVFNNPEL
                     YSNIIKDWSMKDFIPGKESGHDHFFNGFVKYMPIVFDYRPQLLADIVQRAAQQKEQYM
                     EIMIIPDNAHSLTYGDLIKDIPSYDQKRHLLLANKDFQSNIKNTVLESDRILTKASQE
                     LGCKDNPSSDSCHVKIKFIYYVLREQPVDNVFAQALNAFEAVSKSKGNLIGVNLVQPE
                     DGIISLRDYHKHMSIFEYLHRIYPNVPITLHAGELSPQAVTPENLSNHIRDALLIGHA
                     QRIGHGVDIGYENNAEDTLKYMASHHIPVEINLISNLKILNISGRNHPLNYYLTHNVP
                     VVLSTDDEGVLRTDLTQQYVEAAKEHGLNYQQLKQINRNTLTYSFLPGKSIWANADKA
                     ELIPDCHDLGSKSCQKFIEHNEKAKLQWSLEQQLIAFEKNY"
     gene            7380..8495
                     /locus_tag="I9760_000696"
     CDS             7380..8495
                     /locus_tag="I9760_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT5042484.1"
                     /translation="MPYFYTRSAPLFFVFVIALIFINFSYAHEFQTDSKNKHKVDVHI
                     GIYAPFSSHSAFIGRNILVAMEMGSERIQSSDIHYSFYTLDQLPKNKNAGLVLQKFID
                     IHHINVLVTGGKETGLIAAPIAKKNNLIHFSLTNNTEVADGRNNFIVWSPAYDQAKVL
                     VNELKRQQVHHLGIITANQPSSTALTHSVIKQLQADSHIDVAASEYFEPGNKDFSRLT
                     NRIKRKNADMYLVMASPEDIKLIQEQMEADKQHVPITSIVERATPEVVKVFEGQWYVD
                     SHEMQPDFINEFKKNYLNYPVTEAGYAFDLFQLLNKSIVMAIKTQGSPTSQEVVHQLH
                     DIANGTGVMGTMVLDSKGSLFTKSEVKTVKNGEIHTV"
     gene            complement(8567..9904)
                     /locus_tag="I9760_000697"
     CDS             complement(8567..9904)
                     /locus_tag="I9760_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HAT5042485.1"
                     /translation="MVIKKLFSSTLALVYALTLMLPLNAFSAEETNSNYSNTKRIPLE
                     DVQRFSNAIGEIKKYYVKPVDDKELFDNAIRGMLTGLDPHSSYLNEEEFKELQTSTSG
                     EFGGLGIEVTMEEGVVKVITPLVDTPAFKAGIKSGDYIIKLGKESVQGLSLKDAVNLM
                     RGKPGTTIELTILRKGVNKPLTFDLIREVIQIKSVKSKMLSEGYGYIRLTQFQALTGK
                     DMIKAIEQLKQQAGGKLKGLVLDLRNNPGGLLDSAIQVSDAFLGNDKAGKQEMIVYTE
                     GRLPGSKFTALANPGDVLDNAPIVVLINNGSASASEIVAGALKDNKRAIILGTKSFGK
                     GSVQTVLPLDGKTGIKLTTALYYTPSGVSIQAKGIIPDIVVEEVDVPKNAVKKDTLAG
                     FTEADLNGHLINKDNSENTESKNNQIQTGDLIHEDYQLYAALTVLEGMALANR"
     gene            complement(9985..11127)
                     /locus_tag="I9760_000698"
     CDS             complement(9985..11127)
                     /locus_tag="I9760_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAT5042486.1"
                     /translation="MRMSSLSNYLSNAPLGLALMVILFFCNGLEAKTAPNSITQAQTK
                     LKQLDAKINKLKQVLANAQDKRTVLNQELGSTEKQIGEGIRKLRIIQANMSAKEKSIA
                     ELQGKINHLNERLQTQQEMLANHVRARYQMGEYQPLKWLINQDEPFKINRILTYYQYI
                     IRSRQQLIDEIARTRQNLNDSKTKLGNELAENKQLQFKLSQNQKQLEQNKRYHSTLIQ
                     SIDNDIQNNQQSLKDFQKDRDNLSHLMKSLSQQSVIQSGKPFTGMRKKLPLPVQTTHR
                     SLRRMNQGVTFFADEGSIVTAVYPGKVVFSDWLKGYGLLLIIDHGQGFMTLYAHNQSL
                     FKRKGQIVQQNEQVASVGHTGGIKQNGLYFEIRQRGKAVNPLDWLS"
     gene            complement(11114..12658)
                     /locus_tag="I9760_000699"
     CDS             complement(11114..12658)
                     /locus_tag="I9760_000699"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAT5042487.1"
                     /translation="MSHNAPLVLMILDGWGYNENDRYNAIAKANTPQWDEWWQTCPHI
                     LLKASGLPVGLPDEQMGNSEVGHMHIGAGRVIQQDFTRINEAINNGKFAKNAVFHEVI
                     DQLKKTEKSLHIMGLLSPGGVHSHEQHLFALLALCNQKKFRSVHLHLFLDGRDTPPQS
                     ALDSLKCLNEELVKHPVATINSICGRYYAMDRDKRWERVEPVYNLLTQGKSEHQFPDA
                     ETAIHFYYKNKISDEFVPPTLIGKEHSIQDGDAVLFFNFRADRARQLTSTFLDPLFKG
                     FERKTLPKLSYFVSMTQYDKNLITTTAFPPVPLNNTLGEVLSSHGLSQLRIAETEKYA
                     HVTFFFNGGCESVFTNEERIMVPSPQVATYDLQPEMSAPELTKTLIAAINSRDYHVII
                     CNYANADMVGHTGNFEATVQAIECLDQCMHQVWQALKNNGGKLLITADHGNAEEMFSE
                     ATNQAHTAHTSEPVPFLYVGGGWHFTHAEGSLIDIAPSLLALLGITPPPEMTGRILLE
                     KNHAHV"
     gene            complement(12761..13057)
                     /locus_tag="I9760_000700"
     CDS             complement(12761..13057)
                     /locus_tag="I9760_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042488.1"
                     /translation="MSRDTKNLILKAIILFVSLTALVNHAMAFNLLGIGPNPKNLPKD
                     NDVRATFNHCKSCDKSTTPPLIITTPVPIIYYPGCRPIQSCDAYGHCEQKEDCY"
     gene            complement(13134..14405)
                     /locus_tag="I9760_000701"
     CDS             complement(13134..14405)
                     /locus_tag="I9760_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HAT5042489.1"
                     /translation="MKKSIHRLIKPRLFAMSISLLAFSSITYGESNGSFALSEHWSMG
                     QQIKLRFDINEIPQSGVVLHLKNGLALTAGDIISLGDLYGIVGKPISHGLTKLEKQTR
                     FKEVFNSFARNAIAVSEVEDLNSVIRAEMREVESGIAKGETAEAIYKRIGNEVGRQIN
                     CITGGGCNSLTWWLNPGRYLKLAMENYDHFTPNNLIAYKSGHHVALQQALKAKRTGKR
                     SDLEIAYAMDAFACHYLSDHFAAGHLRTPREELSAIITPTVLGALLSNYMHNEENKYG
                     IHVTNNLGEQWMVYGDYSYFNPFNQTNQQMLLKALQASADEIFEAYYSGVLPEKNQIE
                     DMIPHITKLNDENNMDIAPMFYWDKENKQLLRRTNLTNPYDRHWTNSWWGWSTLLLLK
                     TQYGITSTVQLSLSKYLSQYVPEELQDNPLV"
     gene            14755..15471
                     /locus_tag="I9760_000702"
     CDS             14755..15471
                     /locus_tag="I9760_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAT5042490.1"
                     /translation="MNHKLPQHIAVVMDGNGRWAESRGLPRVEGHKAGLDSVKKIINC
                     CLEKKISCLSLFAFSSENWSRPVTEVNFLMELFLEALRKEIDDLNQHGIRLKFTGDRE
                     PLSQVLQKQMCDAEALTKNNQQLILNVVVNYGGKWDIVTAAKKLIHAVLDGKLACDEI
                     NEAVFAQFLDTSGMPEPDLFIRTSGELRISNFFLWQLAYTELYFTDVHWPDFNEHEFE
                     LALTSFARRARRFGQISQSE"
     gene            15482..16279
                     /locus_tag="I9760_000703"
     CDS             15482..16279
                     /locus_tag="I9760_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAT5042491.1"
                     /translation="MFMQRLITTLILAPLVLLAIFYAPSWILWGIVLVILLLAGLECF
                     KLIPVDNLTVQACFLIFLLMCFWASGYFYPYWLSVGLLVWLLNGLAILTYPRSQNYWG
                     YPIVVAIASLILLPLFTQSLIQISFLQQGKELLVYLLFLIWASDIGAYLAGKQWGKHK
                     LIPKVSPGKSWEGVTGGVILAMIVATLGYYYFIPYSIYIWFGLALSTVVISIFGDLFI
                     SILKRRCQLKDTGNIIPGHGGILDRLDSLIAALPWFYFGLTYIPLGI"
     gene            16312..17664
                     /gene="rseP"
                     /locus_tag="I9760_000704"
     CDS             16312..17664
                     /gene="rseP"
                     /locus_tag="I9760_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAT5042492.1"
                     /translation="MLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVLRFSFGFGKI
                     LARFYDKKGTEYAWSLFPLGGYVKMLDETEGEVSEKEKPFAFNNQSVLVRIAIVVAGP
                     LFNFIFAFVALWLVLVIGMHSLAPMIESVKPNSIAARAGLLPKQEILALNGVKINSWR
                     DFQYEIMPLIGSQETVSLTVKSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIVPFIP
                     TIPPIVGEVVPDSPAEKAGLKIGDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIKR
                     QGKLLKITVHTGSQDNNGKLEGLIGVRSQKVDWPAHWLRLEQQPPISALGTAFKQTIQ
                     LTGTTFILMGRLVTGKLGLNSISGPVGIAQGAGDSGRGGLVSYLFFLALVSISLGALN
                     LLPIPMLDGGHLLYYVLEIIRRKPLSDGVKSVGIYFGLLLLVALMFVALSNDISRLTS
                     "
     gene            17835..20147
                     /gene="bamA"
                     /locus_tag="I9760_000705"
     CDS             17835..20147
                     /gene="bamA"
                     /locus_tag="I9760_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAT5042493.1"
                     /translation="MKKVSNKLILGVCCSTLLAWSSQSFSSDTFIVKGIRVNGLQRVS
                     TGTVLNYMPVQVGEEISSSSTAQIIRALYETGFFQSVSLERQGNVLVVNVIERATIGS
                     ITVVGNKEIPSDKMKAFLKEMGLVKGRVFQRSSLERLEKELKQAYTARGKYNSRIETK
                     VTPLTENRVAISITVSEGRVSRIKEIKIIGNHDFKANELLPELTLSTSNLFTYFTKKD
                     QYSKPGMDASLEALRSFYLDRGYLKFNVVSSQVLLSPDKKDVYINIHIEEGPQYHFSG
                     YDVVGKTILPKEKIDSLIQVKKGDVFSRKKVTESISAIGLALGDVGYGFPAINAEPRI
                     DENNKTVFITFVVQPGRHVYVRRINFHGNTKTGDYVLRNVIRQDEGGLLSLHNIKESE
                     RQLRMLGYLKNIDVKTTPVPGTNNQVDLDVNVEEAPSAEASASMGYGTNGYQFNASVN
                     QRNFMGSGRSMGAAFNASQWGQDYSFNYYNPFYTDTGVGRGGSLYYSRIDPKNLNVST
                     YSSNRYGGDISYNFPLGEKSSFQLGYGYQDINIKSVGYVLPIINFVALNGNHFQEIRL
                     TSGWSRNSYDQMPYPNQGFNQQAIAMVALPATSQSLSYYKSSYQAHLYYPLTRGWIFS
                     VLGNVGYGNTFDNFGLPFFENYYAGGPVQPGQVRGYDSYSLGPQDNFGNAMGANFLVN
                     GSVGLILPYPLSRDNVRTTIFADAGNVFASGTPPALRGTPAGPMRYSAGVSLEWRSPF
                     GPLSFSLAKALNPQPLDQTQLFQFALSSGF"
     gene            20261..20761
                     /locus_tag="I9760_000706"
     CDS             20261..20761
                     /locus_tag="I9760_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HAT5042494.1"
                     /translation="MKRLGAVLVALFLGLFGTTVFADWAKIGVVDLQKIMQTSNQMKE
                     IQQKLEKEFKPRRDKLVAMEASLKSDMEKFKRDSAIMSASQKKELEKKIVASQQQFER
                     DGQQYQQELSTAHNEAMEGLYNKVRTAITKIAKDEKYDIIVQKDAAPFSSESLDVTDK
                     VIKAIN"
     gene            20775..21794
                     /gene="lpxD"
                     /locus_tag="I9760_000707"
     CDS             20775..21794
                     /gene="lpxD"
                     /locus_tag="I9760_000707"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAT5042495.1"
                     /translation="MLTLEELANYLNGVWHGNANHAIFSLSSLARASSKDLAYFDNPL
                     LQSVLNSTRAGAVLLKPEHKDWCPVNCIVVPNPSEAMFKAAKSLPHLATNVCGIHPTA
                     QIHKSAQIGQYVSVGANSVIGENVQLDDYVTIGSNTTIESSVLIGRGSQLGSGAIIHS
                     GTVLGQSVIIDSGCIVGAAPFNCYKEHGVWQQAPNFGGVVIGQRTQIGANTVIHRGSI
                     GDTYLGDGVCIDSLVLIAHDVYIGNNTAIAGSAAIGALVQIGSDCIIGGASCLAANIR
                     LTNDVVITGMSTVTKSIARPGIYSSGTTVHDHRKWRRNTARFRHLDDYITKLITIEKK
                     LNNNN"
     gene            21912..22364
                     /gene="fabZ"
                     /locus_tag="I9760_000708"
     CDS             21912..22364
                     /gene="fabZ"
                     /locus_tag="I9760_000708"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAT5042496.1"
                     /translation="MNESIDINQIFTLLPHRYPFILVDRVIDYKVMEYLIAIKNVTIN
                     ENFFTGHFPGNPIMPGVLMLEALAQACGILANLSRQPKEGYEFLHYFAGIDNARFKQV
                     VIPGDQLRLEVRLAGQKRDFWRMHGEAYIGDKLACSADLLSAAKEIKK"
     gene            22361..23131
                     /gene="lpxA"
                     /locus_tag="I9760_000709"
     CDS             22361..23131
                     /gene="lpxA"
                     /locus_tag="I9760_000709"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAT5042497.1"
                     /translation="MIDERAMIHPSAKLASGVSIGPGTVIGADVEIGENTWIGPHVVI
                     EGPTVIGKNNKIFQFASVGDEPQDITYKGEPTRLEIGDNNVIREYCMISRGTVKGGGV
                     TRIGNSNYLMAYSHIGHDCMVGNHIIMVNYAALSGHVTINDYAIIGPYAAVHQFCQVG
                     AYAFIARATYVTKDVLPYVMIAGHTTSACGINTVGLRRRGFSSAAIDCLRRAYKIIFR
                     KGLTVQQAVSELELIQNECPEIIPMIDALNQSTRGIVR"
     gene            23135..23539
                     /gene="crcB"
                     /locus_tag="I9760_000710"
     CDS             23135..23539
                     /gene="crcB"
                     /locus_tag="I9760_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAT5042498.1"
                     /translation="MVVAPYLAVAIGGSLGAMSRYLVTIMAQNAWGIKFPYGTLLVNT
                     LGSFLAGFFLIVLVGRFSAEESFRLFLFTGFLGAFTTFSSFAAESLFMFEQGYWFKLI
                     TNILVNNVGSLSMVFIGTLVAKYVLLGHQGSN"
     gene            23561..24841
                     /gene="serS"
                     /locus_tag="I9760_000711"
     CDS             23561..24841
                     /gene="serS"
                     /locus_tag="I9760_000711"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAT5042499.1"
                     /translation="MLDNQLLRENPQYVATQLLKRGFQFDAVTFSQLEEKRKALQVST
                     QSLQNERNLRSKAIGEAKSRGENIEPMREEVNKLGAKLEQQKTELDEVLKQIEVISLS
                     LPNIPHESVPVGKDELDNQEIRKWGDVPAFSFPVKSHDELGEALGQMDFALAAKITGS
                     RFVVMKGHLARLHRALIQFMLDIHIQQHGYQEIYVPYIVNADSLLGTGQLPKFEADLF
                     KLTGDNGYYLTSTSEIPVTNTVREMILSAEQLPIRYVCHSPCFRSEAGSYGKDTKGMI
                     RQHQFEKVELVWITKPEDSYNALEQLTQHAEVILQRLNLPYRVVALCTGDIGAGSAKT
                     YDLEVWLPSQNTYREISSCSNMEAFQARRMKARFRNPDTNEIQLVHTLNGSGLAVGRT
                     LVAIMENYQDEHGNIHIPDALKPYLGGIDIISVK"
     gene            25100..26143
                     /locus_tag="I9760_000712"
     CDS             25100..26143
                     /locus_tag="I9760_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5042500.1"
                     /translation="MNEVIIREITSEDKEMFLCAMQSSHALHHPWVKAPITPEEFDEY
                     FSRYQKPNQKSYLLLSDNNLAGVFNISEIVRGYFQNAYLGFYAVAAYAGKGYMSTGLK
                     LILTKVFKDIGLHRLEANIQPENTRSIWLVKKNGFRYEGFSPRYLRVNGVWQGHEHWA
                     MTYEDFIKDNNEALEKDHINIVAYNTEWPLLAKAEMVKLRASFPANSVIDIQHVGSTA
                     IPGMASKPIIDIQIAVRSLEEMKIIAVPILQKLAYEYWEDNPDHERMFFVKGMPPYGN
                     GRTHHVHIVETSSRHWKGKIFFRDYLLSHPEAAEKYQQLKIKLAQEHKYDREQYTDAK
                     SEFINQILKLAQN"
     gene            26314..26577
                     /locus_tag="I9760_000713"
     CDS             26314..26577
                     /locus_tag="I9760_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042501.1"
                     /translation="MADQPPKSKWHEIKRLSEGDNKVDNNQAPVSTADRIKKLYLDTQ
                     VGSPIDTVEEADAQRNNLGPKDTKIENSSPENDEPNPDIPTPG"
     gene            26917..27849
                     /gene="legD2"
                     /locus_tag="I9760_000714"
     CDS             26917..27849
                     /gene="legD2"
                     /locus_tag="I9760_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegD2"
                     /protein_id="HAT5042502.1"
                     /translation="MDNLLNEKKLTIFTSNEIQDNLNRVLKNYEQNGIIGLNGYLNQA
                     NRISLLNELEIIQKDAEQDIVRYWNNKIIHFYSKDPLKPESVDREYVTEPYFQASSNK
                     AHVFYEVIDNMRVVNRIGHGMHLINRYPMMQRAVYDSPVLLDLLKGIGFKKPICHLSV
                     YIPKYPNGIGSEVKPHQESTFAFTEPQSVVVLWVALEDALIENACMYGVLGSNHWPLK
                     WVSKVDRETKTRHFEQVHQLHIPDFMTEREFYTALEVKAGDALLFHGNFVHCSPMNTS
                     KNSRKALSFQFIETYEVDYPETNWLYPPNKVYLY"
     gene            27885..28697
                     /locus_tag="I9760_000715"
     CDS             27885..28697
                     /locus_tag="I9760_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5042503.1"
                     /translation="MSMYDKRQFFKTYFSSASEKSILVKLLDKRFHPPFHTANILDLG
                     CHDGALMKNIVNAYAKRLPEKLVITGVDPSLQAIKEYAKADFPIPVQTNTFAGTAEDY
                     FDVCTDHFDWIFASQCLYWSPDLPKIIKQIHEAGDSALIVIRGCHGIYEIQSQFRHYI
                     GNHLEKFYTDKEVEMALRTQKILFQKESLSTFIRLPDKDSIELKWLILFFLQMEEESL
                     GDKIFCEVRDWITSRASGSIHHEVHFFWLGNAIFTGDYNRNFPVKSKKNSRG"
     gene            complement(28687..29517)
                     /locus_tag="I9760_000716"
     CDS             complement(28687..29517)
                     /locus_tag="I9760_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAT5042504.1"
                     /translation="MDLGFPTFIYGTAWKEKRTSELVELALTTGFRGIDTANQRKHYY
                     EEGVGYALQQYYKKSGILRDDIFLQSKFTYAHGQDHRKPYDDTASLTIQVQQSFASSL
                     EHLNTDYLNSYILHGPYYQYHFSDEDKEVWNKMESLHKDGKVKHLGISNVSYEQLIEL
                     ISFASVKPKFVQNRCFARLLWDKQIRNYCQAHGIIYQGFSLLTANQYELRHPKLENLS
                     KKYEKSIEQIIFRFSQQIGMLPLTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP
                     "
BASE COUNT         8795 a         5152 c         6201 g         9375 t
ORIGIN      
        1 tctcgatcat gtatattcaa aaaatgcata aatacgggat tcatattcaa tgagaaaagt
       61 ttttagttta ttgttaatta gttcaattat agttatatcc agttgtagtg agcaaaaaaa
      121 tcaaaataca ctgcgttttt ccacttcagc agaatatcca ccttttgaat atattgaaaa
      181 cggagaaata aaaggatttg acattgattt ggcaaaattg atcgccaaag agctaggtaa
      241 agaagcagtt tttgacaaca tgcagtttag tgcggttttg cctgctgtga attcaggcca
      301 ggatgacgtt gcgattgcca caattactat taccgaggaa cgtaagaaaa attttgattt
      361 ttccaaacct tattattttg aaggaatggc tgcagttttt gatacttcac agccagtgaa
      421 agagatctcg caattaacag gaaaaaaaat tgcagtccag ctgggtagtg ttatggaaat
      481 ttggcttcat caaaactttc ctaaagctga aattacggct ttggataata acaaccaagc
      541 aatagaagct ttaatcgcgg gtcatgttga tgtggtcctt atggatgggg cacaaggggg
      601 tgtttatagc aagaaatatc caggactgtc ttattctatc ctggcgaaag cgaatgaagg
      661 ttatggattg gtcttaaaaa aaggttctca tctaagagat gaaatcaatt ccgcattgga
      721 aaatcttaaa gcgaagggtg aaattcaaaa attggaatcc atttggataa aggggtcaat
      781 atgacggaga tagtgcaaaa tctgtttttc attggcaaag gaatagttct aactatgcaa
      841 ttattaacag gaggtatgct gattggagta ttacttggaa ctctcttgtc aatttgcaga
      901 catcaaggag tagcttgctt tgttataaac cgattcatat ccatattgag aggcacacca
      961 ctcattttac aattaagttt tatttatttc gcagtgcctg ttttattggg tatgcgacca
     1021 agtattttag tggctggaat tgttgctttt ggtttgaata gctcagctta tttcgccgaa
     1081 attttaagag ccggaattga gagtttaccc aaagggcaat ttgaagcagc ccttactctg
     1141 caaattccta ccttttactt atggaaagat attattttac ctcaagtgat aaaaaatatt
     1201 tttcccgcaa tgatcaatga aatgatcgcc ttgttaaaag aaacggcctt gatttccact
     1261 attggaggca tggatttaat gcgtcaggct caaactttgg cagcacaaca atttacttac
     1321 tttatgcctt tatgtatcac gggtttctat tattacagtt tggtcttact ccttgaagca
     1381 ctaggtaaaa aaattgaaaa aagggggcat tatgctgcaa gtcattaacg catcgaaaaa
     1441 aatcggcaat gcccaagtac ttaatgagat taatttaagt attcaagcaa acagtattgt
     1501 tggtttagtg gggccttctg gcagtggaaa aacaacttta ctgagatgca tacaacaatt
     1561 ggagtcaatc gattcaggct ctatccattg tgaaggaaaa agcggtttta tgtttcagga
     1621 ttttcaatta tttccgcaca tgacagtact caataaccta atttatgcac catatcttca
     1681 cgataaaact gctgatcata tggcaaacgc gtgggcttta cttgctatgt taggtttgtc
     1741 agacaaagct cattcttacc cacaaggatt gtctggtgga caaaaacaac gcgtggcctt
     1801 ggctagaagt ttaatgatga aaccggattt attgctttgt gatgaaccga cttctggtct
     1861 agatatggcg accactaatg atgtcattgc tttgttaaat acagtgaaga ctctaaaaat
     1921 cactatgctt atcgcttctc atgatttgtt ttttttatcc aatattgcgg agcgattgtt
     1981 ggttgtaaag caagggcaac taattgctga ctttaaaacc caagaggtgt ctgatcctat
     2041 tttatatcta cagcagtttt accaggggga aatattatga aaaaggttat taaaaaaatc
     2101 gcattagctt attcaggtgg cttggatacc tccatcatga tcccttggct taaagagcat
     2161 tatgagcatg ctgaagtaat cgctgtgatt tgtgatttag ggcaacagga agatctcgat
     2221 accataaaaa ataaagcgct aaaaagcggc gcttccaaag cgtatgtagt cgatgtgaag
     2281 aatgagtttg ccattcaata tctttggcct ttggtaaaat caggtgcttt atatgaagat
     2341 caatacattt tgggaactat ttcaaggcca ctaattgctc aaaaacttgt tgaaattgct
     2401 ttgacggagg aagtaaatgc tgttgcgcat ggagccaccg gcaaagggaa tgatcaagtg
     2461 cgttttgaat attcgattaa ggcacttgca cctcagcttg aaattattgc tccctggaga
     2521 acttgggata ttaaatcaag acaggaagca attgtgtatg ctaaagcgca tggcattgag
     2581 gtaccagtga ctcccaaagc gccttattct agagatcata acatttggta tatttctcat
     2641 gaaggtggtg tgctcgaaga tccttctcag gaaatgccgg atgacgtgtt attaatgact
     2701 gctcctgtat cacagactcc tgatgaagag gaagtggttg ttcttgattt caaaaagggg
     2761 gttccagtcg ccttaaatgg acaagaactt tctccagtgg atctcttaaa ttcattaaat
     2821 caaaaagcag ggcagcatgg tattggagtg gcggacattg ttgaaaatcg attagttgga
     2881 atgaaaattc gagggattta tgaagcacct gctgctgcag tgctttataa ggcacataaa
     2941 cttttggaga gtttatgtct cactcgttct acgttacatc tgaagcaatc tttgcagcaa
     3001 acttatgcca atttggttta tgaaggaaga tggttttctc aaaccaaaca ggctttggat
     3061 gcattcattg atgtgacgca gcagcatgtc acaggctgtg ttaagttaaa gttattcaaa
     3121 ggaaacatta tccctgccgg tatgcattct ccctatagct tgcatcatcc cgaattggct
     3181 acttttgaag aggataatgt atacaaccaa aaggacgctg aaggattcat taacctcttt
     3241 tctttatccg ctaaaatcta tagccaggta catcaggagg ggaattatga ctaataagac
     3301 atggggaggg cgatttaaga aatctttgga tagcggtgtc aaccagttta atgcgtcatt
     3361 gtcttttgat catgtgcttt ttgaccaaga cattaatggc agtcaggttc atgtgaagca
     3421 attagctaaa cagaagattt taacagaagt tgaaagccag gcgatttatt ctgctcttga
     3481 agaaatcaga agcgagataa aacaaggcca atattcattt aatgtacgtg atgaagaaga
     3541 catacatatg tttattgagc aattactgat tcaaaaaata ggcgatttgg gtaagaaatt
     3601 acacaccggc cgtagtcgta atgatcaagt ggctttggat cttcgtttat ataccagaga
     3661 taaaggctgt ctgattaatg aacttttgac aaggcttatc gattgtctag atgatctaac
     3721 cagtaaacat caacaggatt taatgccagg ctatactcat ttacagcaag cccaacctgt
     3781 tacgttaggg gcttatttta acgcgtatca atgcatgttt ggccgagata aaagcagatt
     3841 ggaagattgg tttaaacgca tgaattattc tcctttgggt gctggggcac tggctggaag
     3901 taccttgcct cttgatagag aatgggtagc cgaatccttg ggatttgcag ggatcattcc
     3961 taatactctt gatgcagtca gtgacagaga ttttgtcatc gagttatgca gtgttgctgc
     4021 aatgattatg atgcatcttt ccagattatg tgaagatttg attctatggt ctacccagga
     4081 atttaatttt gttatattgg atgatgcgtt tgctacaggc tcttcactga tgcctaacaa
     4141 gaaaaatccc gatgttcctg aattgattcg tggcaaatca ggacgagttt atggtcatct
     4201 aatggccatt ttaacggtaa tgaaagggtt gcccttggcc tataacaaag acatgcagga
     4261 ggataaagaa ggattatttg atacgattaa tacgataatc gcttgcctgc aaatgataac
     4321 tcctttcttg caaagtctga cttttaatac cctgttaatg aaaaccaaag ctcaaagtgg
     4381 ttaccttgat gcgactgcca ttctggagtc attagtgatg aagggtatgc cttttcgtga
     4441 tgcacaccat caggttgggg catggattgc agaagcgata gaaaaacaat gttcactgaa
     4501 tgaattatta aaaggaggct aagaatgtct ttttccacct caaccaattt gactccaatt
     4561 gaaacgcaag acgatgacct tgaaaaaatt caatctcaga ataacccatc tgcaattaat
     4621 gaagtgaaga ccaatacttt tgtcattaat ttacaacaag ctatttttgg cttggagaca
     4681 ggtagtgagg ccgttgattt tgattctcat aagaatacag gctttccatt tcctgctggt
     4741 gaatacaaac caagacattt tcttactggc acagagttag caagggagga aattcagttt
     4801 atcttaagcc aggctcgctc tctaaaacaa aacccgggat tatataatca ggcacttgcc
     4861 aataaaagtt tggtaatgat ttttgaaaaa ccttccttta gaacccgttt gagttttgct
     4921 ttagcgattc agagtatggg ggggatcgtt gttgaaagca tcgctaactc aagaaagcag
     4981 gagactccag ctgatatggc tagagtttta aatggttatg ctgattttgt aatggtcaga
     5041 acacatgagg acagtttttt gcaagaaatg gccgaatatt ccaaaatccc tattattaat
     5101 ggcctctccg cattgcatca cccttgtcag actcttgctg acttgttaag tcttgaagaa
     5161 caatttggtg ctttggaggg attaaccctc acttacatag gggatggcaa taatattttg
     5221 catagtttga tgcttttggc accgcagtta ggtgtcactg tgcattattg ttgcccaaaa
     5281 tcaaatcagc ctaatgaggc cattattaag gaagcccaaa gcaagacagg gaaacaaatg
     5341 atttattgtt ttgataaacc ggaggaagct gtgaagcaag cggatgccgt ttataccgat
     5401 gtgtggacaa gtatgggatt tgataatcct gaagcgggca agatttttaa agggtttcag
     5461 attaatgaga cattaatgtc tcatgcaaaa cccgattcgg tgtttatgca ttgtatgcct
     5521 atggaaaggg gaaaagaggt ttctgccact ttacccgaca gcgcgtcatc tatcattttt
     5581 aggcagagtg agaatagaat gcatgtacaa aaggctttga tgctattttt ggatcaatag
     5641 aactttggat tatgaggccc atatttgttc atgatgaaac gaaattgcgc atttctcctg
     5701 atatgggctc atgatttttg caaattaatt tagtaattct tctcaaaggc aattaattgt
     5761 tgttccaggc tccattgcaa ttttgccttt tcgttgtgct cgatgaattt ttgacagctt
     5821 ttgcttccaa ggtcatggca atcaggaatc aattcagctt tatccgcatt agcccatatg
     5881 ctttttccag gtaagaatga gtaagtcagt gtatttctat taatttgttt taattgctga
     5941 taattaaggc catgttcttt ggctgcctct acatattgtt gggttaagtc cgttcttagc
     6001 actccttcgt catcagtgga taaaacgact ggtacattat gagtaagata ataattaaga
     6061 ggatgattgc ggccggatat attcagaatt tttaaattac taatcaagtt tatttcaacg
     6121 ggtatgtgat ggctagccat gtattttaag gtgtcttctg cattgttttc ataaccgata
     6181 tccacaccgt ggccaattct ttgggcgtgt ccaatcagca gtgcatcgcg aatatgatta
     6241 cttagatttt ctggtgtaac tgcttgagga gataattctc cggcatgcaa ggtaatagga
     6301 acattcgggt atattcgatg cagatattca aagatggaca tgtgcttgtg gtaatcacgc
     6361 aaagaaataa ttccatcttc cggttgcacg aggtttacgc cgattaagtt ccccttagac
     6421 ttggagacgg cttcaaaggc attcaaggct tgggcaaaga cattatcaac gggctgctct
     6481 ctgagtacat aataaataaa tttaattttt acatggcagg aatcggaaga agggttatct
     6541 ttgcagccta attcttgaga tgctttggta agaattcggt cgctttccaa aacagtattt
     6601 tttatgttgc tttgaaaatc tttattcgct aacaacaggt gccttttctg gtcatatgat
     6661 ggaatgtcct tgattaaatc accataagtt aatgagtggg cattgtccgg gattatcata
     6721 atttccatgt attgctcttt ttgttgtgct gctcgctgaa caatatcggc aagcaactga
     6781 ggcctataat caaaaactat gggcatatat ttgacaaatc cattgaaaaa atgatcgtgg
     6841 ccagattctt taccaggtat aaagtctttc atagaccagt ctttaattat gttggaatag
     6901 agttcaggat tgttaaaaac ctctttaaca ttaactccct cacaatgaga agaatcttta
     6961 cttacggcaa atgagtgttt atcgaggcaa tagttgccat ttgcggcaag tgccagcatg
     7021 gtctcaggat atgctcctcc agccaaatga taatgtaact cgccaccctt gggcattgtt
     7081 ttaaaaaagg cataaagggc actggaatcc ttgtttatat gactaaaata ggaatcaaca
     7141 ctggcataaa ttgggcacaa aaggaagaaa ctggtaaaaa atgcgagtaa tctggtaatg
     7201 ttcataattt caatgtgatc attaattggg cagatattat ctcataatct tgcacgattc
     7261 ccaacagaat atctgtttta tcatgcaatc tcttatgatt ttttacgaaa tgataatgat
     7321 tgtatttttt tattattcat tgcagaatgt ttttaaggtt taacacatgg aagaaaaaaa
     7381 tgccctattt ctatacaaga tcagcgcctt tgttttttgt attcgttatc gcacttattt
     7441 ttataaattt ttcctatgcc catgaatttc aaactgattc aaaaaataaa cataaagtgg
     7501 atgttcacat aggtatttat gctccgtttt ccagtcattc agcttttatt ggaagaaata
     7561 ttctggttgc aatggaaatg ggcagcgaac ggatacagtc ctccgatatt cattattcat
     7621 tttatactct ggatcaactt cctaaaaata agaatgctgg tcttgtgctg caaaaattta
     7681 ttgatatcca tcatatcaat gtgttggtga ccggaggtaa agaaacgggg ttaatagcag
     7741 ctcccatagc aaagaaaaac aatttgattc attttagtct gaccaacaat actgaagttg
     7801 cagacggcag aaataatttt atcgtctgga gtccagctta tgaccaagcc aaggttttgg
     7861 ttaacgaact aaaacgtcag caggtccatc acttgggaat tattacggca aatcaaccgt
     7921 cctcaaccgc tttgacacat agtgttataa aacaattgca ggctgattct catattgatg
     7981 tggctgcctc cgagtatttt gaacctggaa ataaggattt ttcaagatta actaacagaa
     8041 ttaaaagaaa aaatgcagat atgtatttgg taatggcatc acctgaggat attaaattaa
     8101 tacaagaaca aatggaggca gataaacagc atgtgcccat taccagtata gttgagcgtg
     8161 ctactccaga agtggtaaag gtatttgaag gtcaatggta tgttgattct catgagatgc
     8221 agcctgattt tattaatgaa tttaaaaaaa attatttaaa ttatcctgta actgaggcag
     8281 gttatgcgtt tgatttattt cagcttttga acaaaagcat agtaatggcg ataaaaacac
     8341 aaggttctcc tacaagccag gaagtcgttc atcaactcca tgacattgcc aatggaacag
     8401 gcgtaatggg aactatggta ttggacagta agggttcttt atttaccaaa tctgaagtga
     8461 aaactgtgaa aaatggcgag attcatactg tctaggtaga aaataaaacc tgaaatcatc
     8521 agttcattgt gaatcgatga tttcaggcgg gaggactaat tggtcattat ctgttagcta
     8581 acgccattcc ttctaatacg gtgagtgccg catataactg ataatcctcg tgtattaaat
     8641 ctcctgtttg aatttggttg tttttggatt cagtgttttc tgaattgtct ttgttaatca
     8701 agtgcccatt aaggtccgct tcagtaaaac cagccagggt gtcttttttg actgcatttt
     8761 taggaacgtc cacttcttcc acaacgatgt caggaattat tcctttggct tgaatggaga
     8821 ctcctgatgg agtgtaataa agtgctgtgg ttagttttat tccggttttg ccatcaagag
     8881 gtaatacggt ttgaacggat ccttttccaa agctctttgt accaaggatt atagctcttt
     8941 tattatcttt taatgcccca gcgacaattt ctgaagcaga ggcagaaccg ttattaatta
     9001 agacaacgat aggcgcattg tctaacacat cccctggatt ggccagggcg gtgaatttag
     9061 agccaggtag tctgccttcc gtatagacta tcatttcttg cttacctgcc ttgtcattgc
     9121 caagaaaagc atcggatacc tggatagctg aatcaagtaa acccccgggg ttatttctta
     9181 aatccaatac taatcctttt aatttcccgc ctgcttgttg ttttaattgt tcgattgctt
     9241 taatcatatc ttttccagtt aaagcctgaa attgagtcaa tcgaatgtaa ccataacctt
     9301 cgcttagcat tttgcttttt acacttttta tttgaataac ttcgcgaatt aagtcaaaag
     9361 ttaaaggttt gtttacacct tttctcaaga tagttaattc tatggtagta cctggtttgc
     9421 cgcgcattaa attaacagcg tctttaagag aaagaccttg aactgactct ttacccagtt
     9481 taataatata atcgccagat ttgattcccg ctttaaatgc tggagtgtct acaagaggag
     9541 tgattacttt aacaacacct tcttccatgg tcacttcgat acctaaacca ccaaattcac
     9601 cgctggttga tgtttgcagc tccttgaatt cttcttcatt gagataacta gaatgcggat
     9661 caagaccggt tagcatcccg cgaattgcgt tatcaaataa ttctttatca tcaactggtt
     9721 tgacatagta cttctttatt tcaccgatgg cattggaaaa ccgttgcaca tcttccaacg
     9781 gaattcgttt tgtattagaa taatttgaat tggtttcttc agccgaaaac gcgttcaaag
     9841 gcagcatcag agttagggca taaaccaatg ccaaagtact ggaaaaaagt ttcttaataa
     9901 ccatagcgat ctcctcagat gcaaggccat gaaattagcc acctgcgtat ttttttatgc
     9961 aatttaaatg ccagcggcta agctttaaga caaccagtct aacggattga ccgcttttcc
    10021 tctttgtctt atctcaaagt atagaccgtt ttgcttaatt ccaccggtat ggcctacact
    10081 tgcgacttgc tcgttttgtt ggacgatttg tcctttgcgt ttgaagagag attgattgtg
    10141 tgcgtataaa gtcataaacc cttggccatg gtcaataatc agaagcaaac cataaccttt
    10201 tagccaatca ctaaacacaa ctttccctgg gtaaacggca gtaactatgg agccttcatc
    10261 ggcaaaaaat gtcactcctt gattcattct gcgtaaagat ctatgggtgg tttggacagg
    10321 taacggcaat tttttgcgca ttcccgtgaa cggtttgccg ctttgaatta cactttgttg
    10381 agataatgac ttcatgagat gagataagtt gtctctatct ttttgaaaat cttttaatga
    10441 ttgttgatta ttttgaatgt cattatcaat ggattgaatc agggtgctat ggtatctttt
    10501 attttgctct agctgttttt gattttgtga caatttaaat tgtagctgct tgttttctgc
    10561 aagttcattt ccaagcttgg ttttactgtc gttaagattt tgtcgggttc gagcaatttc
    10621 atcaattaac tgctgacgtg atcttattat atactgataa taggttaaaa ttcggttgat
    10681 cttaaaaggc tcatcttggt taatcaacca tttcaaaggt tggtattccc ccatctgata
    10741 acgcgccctg acatgatttg ccagcatttc ctgctgagtt tgcaatcgtt catttaagtg
    10801 atttattttt ccttgcagct cggcaatgct tttttctttt gcggacatat ttgcttgtat
    10861 gattcttagc ttgcgtattc cttctccaat ttgtttttct gtgctgccca gctcttggtt
    10921 tagtacagtc cgtttatcct gtgcattggc taaaacttgc tttaatttgt taatcttggc
    10981 atctaactgt tttagtttgg tttgtgcttg agttatagaa tttggagcag tttttgcctc
    11041 aaggccatta caaaaaaata gaatgaccat aagtgccaaa ccaagaggag cattggacaa
    11101 atagttggat aggctagaca tgcgcatggt ttttttccag aaggatacga ccagtcattt
    11161 ccggaggcgg agttatccct aataatgcaa gtaaagaagg tgctatatcg attaaacttc
    11221 cttcagcatg agtgaagtgc caccccccac ctacataaag aaaaggaacc ggttcactag
    11281 tatgagctgt atgagcttga tttgtcgctt cgctaaacat ttcctcagca tttccatgat
    11341 cagcagtgat caataattta cccccattgt ttttaagggc ttgccaaacc tgatgcatgc
    11401 actgatcaag gcattcaata gcttgaacgg tagcttcaaa attccctgtg tgtcccacca
    11461 tgtcagcatt ggcataatta caaataataa catgataatc tcggctattg atggcggcta
    11521 ttagagtttt agttaattca ggtgcgctca tttctggttg cagatcatat gttgccactt
    11581 gcggagaggg taccataatg cgctcttcat ttgtaaaaac actttcacat ccgccattaa
    11641 agaaaaaagt aacgtgagca tatttttcag tttcagctat gcgtaattga ctgagaccat
    11701 gagaagacaa gacctcaccc agggtattat ttaaaggaac aggtgggaat gctgtcgtgg
    11761 taattaaatt tttatcgtat tgggtcatgg agacaaaata ggacagcttt ggcagtgttt
    11821 ttctttcaaa tcctttaaac aaagggtcaa ggaatgtaga ggttaattgc cttgctctgt
    11881 cagcacgaaa attgaaaaac aggacagcat ctccatcttg tatagaatgc tcttttccta
    11941 taagagtggg tgggacaaac tcatcagaaa ttttattttt gtaatagaaa tgtattgcag
    12001 tttctgcatc aggaaattgg tgctcacttt tgccttgtgt taataaatta taaacaggct
    12061 ctactctctc ccaacgtttg tctctgtcca tcgcataata tcggccacaa attgaattaa
    12121 tggttgcaac agggtgttta accagttctt cattcaggca tttcaagcta tcaagagcac
    12181 tttgtggtgg agtatctcgg ccatcaagaa acaaatgcag atggacggat ctgaattttt
    12241 tttgattaca aagggcaagt aaagcaaaca aatgttgttc gtgactgtgc actccgccgg
    12301 gagagagtaa ccccatgatg tgaagagatt tttctgtttt ctttaattgg tcaattactt
    12361 catggaaaac agcattttta gcgaattttc cgttatttat cgcttcatta atgcgagtaa
    12421 aatcttgctg tatcactcgg cccgcgccaa tgtgcatatg cccaacttct gaattaccca
    12481 tttgctcatc aggtaaacca acaggcaagc cggaggcttt caaaagaata tgagggcagg
    12541 tttgccacca ttcatcccac tgaggggtat tggcttttgc aatagcattg tatctatcat
    12601 tttcgttata gccccagcca tcaagaatca tgagtaccag gggagcatta tgcgacatga
    12661 cctaaatcct aaattgtaca atgaaagggc taaaatgata attatacctt cgcggcagta
    12721 agaggtatat agtttcagta agagttcgct gttattaaat ttagtagcaa tcttcttttt
    12781 gttcgcagtg accataagca tcgcatgact ggatcggtcg acacccaggg taataaataa
    12841 tgggtacggg agttgtaata attaaaggag gagtagttga tttatcacag gacttacaat
    12901 gattaaaagt agctcttaca tcattgtctt tgggtagatt ttttggattt ggtcctatcc
    12961 ctaatagatt gaacgccatg gcatggttaa caagagcagt taagctgaca aataaaataa
    13021 ttgctttgag gatcaggttc ttagtatccc tactcatgat gtgaactcct ttttaatttt
    13081 atgccccgta ttatgcgata ataagagtat agttgcttgt tatggtcctg ctcctatacc
    13141 aaaggattat cttgcaattc ttccggtaca tattgagaca aatatttgct taaactcaat
    13201 tgcacggtcg aggttatccc atattgcgtt tttaacaata ataaagtact ccagccccac
    13261 caactgtttg tccaatgcct gtcataagga tttgtcaaat tggttcttct taacaactgt
    13321 ttgttttctt tatcccaata gaacatagga gcaatatcca tgttattttc atcatttaat
    13381 ttggtgatgt gtggaatcat gtcctctatt tgattttttt ctggtagaac gcctgagtaa
    13441 taagcttcaa atatttcatc agcggaagct tgcaaggcct ttaagagcat ctgctggttg
    13501 gtttgattaa aaggattaaa ataagaatag tcaccataaa ccatccattg ttctcccaga
    13561 ttattcgtca catgaattcc atacttattt tcttcattat gcatataatt ggataacaac
    13621 gcaccaagga ctgttggtgt gataatggct gataattctt ctctaggggt gcgcaaatgg
    13681 ccggcagcaa agtgatctga taaatagtgg caggcaaaag catccatcgc ataggcgatt
    13741 tccaaatcag agcgttttcc tgttcgtttt gcttttaatg cctgttgcag tgcgacatgg
    13801 tggccacttt tataggctat cagattattt ggcgtaaagt gatcatagtt ttccatggct
    13861 agttttagat atctgccagg atttaaccac caggtcaggc tattacaacc acctcccgtg
    13921 atgcagttga tttggcgacc tacttcattt ccaattcgtt tataaatggc ttcagcagtt
    13981 tctccttttg caatccctga ttcgacttcg cgcatttctg ctctgataac ggaattaaga
    14041 tcttctacct cactaactgc aatggcattc ctggcaaaac tgttgaaaac ctctttaaaa
    14101 cgagtttgtt tttccagttt tgttaagcca tgactaattg gttttcctac aattccatac
    14161 agatcaccaa gacttatgat atcgccggct gttagtgcca atccattttt taaatgtaat
    14221 acaacacccg attggggaat ctcgtttatg tcaaatcgta gtttgatttg ttgccccatg
    14281 gaccaatgct cggacaaagc aaaactgccg ttcgattcac cataagttat agaggaaaaa
    14341 gccaacaagc taatgctcat tgcaaataac cggggcttta ttaatcgatg gatagatttt
    14401 ttcattatgt cctcatcaac agggattgat cattggtttt agtgagaaat agcgtattgt
    14461 ataacgtttg agatgaaatg ttgatgcatt ggttcgtccc gagataatgc ttaggtgtga
    14521 atggagtctg tacaaataac cgtaaccaaa aacgacatag agcgctatag gtcattggaa
    14581 ttatctcctt tctggatatt catgatgaaa agtgacaact cgttacgaag atttattcag
    14641 gtttaataat gaggtgtttc ataaacaagg tcaattgatt tcttaagtag tacttaaatt
    14701 tttatctcaa agaggttaga ctatgcatca tttttattag tcggataagt tgttttgaat
    14761 cataaattac ctcaacacat tgccgttgtc atggatggaa acgggcgttg ggctgaaagc
    14821 cgcggtttgc cccgtgtaga gggacacaaa gcgggccttg actcagttaa aaagattatc
    14881 aattgttgcc tggaaaaaaa aatttcctgc ttaagcctgt ttgcatttag ctctgaaaat
    14941 tggtcaagac cagtgacaga agtcaatttt cttatggagt tgtttttgga ggctttgcga
    15001 aaagagatag acgatttaaa ccagcatgga atacggttaa agtttactgg cgatagagaa
    15061 cctttgtctc aagtattaca aaagcaaatg tgtgatgcgg aagctcttac caagaataat
    15121 caacaattaa ttctcaatgt tgttgtgaat tatggtggga aatgggatat agtgacggcg
    15181 gctaaaaaat taatacacgc tgttttagat gggaagttgg catgtgatga aatcaatgag
    15241 gcagtattcg ctcaatttct tgatacgagt ggtatgccgg aacccgatct gtttattcga
    15301 actagtggtg aattaaggat aagtaatttt tttctttggc agttagctta tactgagctg
    15361 tattttactg atgttcattg gcctgatttt aatgagcatg agtttgaatt ggcattgact
    15421 tcttttgcca ggcgggcaag aaggtttggg caaatttcac aatctgaata aaggtaaaac
    15481 catgtttatg caacgattga tcacgacatt aatattagcg cccctggttt tgctagctat
    15541 tttttatgcc ccttcctgga ttttatgggg tattgttcta gttatactcc tgcttgcagg
    15601 actggaatgt ttcaaattga ttcctgttga caatttaact gtacaggctt gttttttgat
    15661 atttttattg atgtgctttt gggccagtgg ctatttctat ccctattggt tgagtgtggg
    15721 cttattggtt tggttattaa atgggttagc aattcttact tatccgcgtt cacaaaatta
    15781 ttggggttat ccaatcgttg ttgccatagc ttctttgata ttactaccct tatttactca
    15841 aagtcttatt caaatttctt ttttacagca aggaaaggaa ttacttgtct atttgctttt
    15901 tttaatttgg gcttctgata ttggtgctta tctcgctgga aaacaatggg gaaaacataa
    15961 attaatccct aaagttagtc cgggtaaatc ttgggaaggt gttacaggcg gagttatttt
    16021 agccatgata gtcgcgactt tgggctatta ttattttata ccttactcaa tttatatatg
    16081 gtttggttta gcattgtcta ctgtagttat ttcaattttt ggtgatttgt tcatcagtat
    16141 attaaaacgg cgttgtcaat taaaagatac tggaaatatt attccaggtc atggcggtat
    16201 attagatcga ctggatagcc taattgccgc gttaccatgg ttttactttg gattaaccta
    16261 cataccactg ggaatttaat tttctggtcg tatcaatctt tggataacga tatgctttca
    16321 acgttgctgt actttttatt agcattggta ttgttagtca ccatacatga atacggccac
    16381 tttcaagtag cgcgatggtg tggtgtgaaa gtgttaaggt tttcatttgg ctttggaaag
    16441 attcttgcgc gtttttatga taagaaagga acagagtatg cctggtcttt gtttcctttg
    16501 ggtggctatg tcaaaatgct tgatgaaaca gaaggagaag tttctgaaaa agaaaagcct
    16561 ttcgcattca ataatcaatc tgttcttgta aggatagcta ttgtagtagc agggccttta
    16621 ttcaatttta tttttgcgtt tgtagcattg tggttggttt tggttattgg tatgcactct
    16681 ttggctccga tgattgagtc agtaaaacca aatagtattg ctgcacgagc tggtctactc
    16741 cccaagcagg aaatccttgc tcttaatgga gttaaaatta atagttggcg agattttcaa
    16801 tatgaaatta tgcctcttat cggctctcag gaaacagtga gcttgactgt aaagtcaatg
    16861 ataaatggtg aagaaagcac gttgtatttg cctttggata agtggcagtt ggatgataaa
    16921 aagcctgatc ctattaaaag cctgggcatc gttcctttta tacccactat tccacccata
    16981 gtcggtgaag tggttcccga ttctcccgct gaaaaagcag gcttaaaaat aggggatgaa
    17041 attattagtg tcaatggtca gcatttcaat gattggttgt atttagtcag ctatgtaagg
    17101 gaaaggccca attctcaaat caatttggat attaaacgcc aaggtaaatt gctgaagata
    17161 acggtacaca ctggaagtca ggataataat ggaaaattag aggggttaat aggagttaga
    17221 tctcagaaag tagattggcc tgctcattgg ctacggctgg agcaacagcc gccaatttca
    17281 gcacttggta cggcttttaa gcaaacaata caactcacag gcacaacttt tatcttaatg
    17341 ggaagattag tgactggaaa attgggatta aacagtatta gtggtcccgt aggtatagcc
    17401 cagggggccg gtgattccgg acgcggagga ttggtatcct atttattttt tctggcatta
    17461 gtaagcatta gcttgggagc gcttaattta ttaccaatcc ctatgctgga tggcggtcat
    17521 cttttgtatt atgtcttgga aatcataaga agaaagccac tatcggacgg agttaaatct
    17581 gtcgggatct attttggatt attactgttg gttgccttaa tgtttgttgc tcttagtaat
    17641 gatatatcaa gattaaccag ttaggatttg gcgagagtaa ttctaatttc tgtatgatta
    17701 atgaatgaaa tgatgtttta gcgacaaaga aacttgacag tagtttctag ttcctataaa
    17761 agggtttcaa ttttttgtac aattgaatgg tttttaattt cataagtgcg cgtagtgctg
    17821 gacgtaaaat aataatgaaa aaagtcagta ataaattaat attaggtgtt tgttgttcta
    17881 ctcttttagc ttggtcatcc caatcctttt cttctgatac ctttattgtc aaagggatta
    17941 gagttaacgg gttacaaagg gtttcgacag gtacggtatt aaactatatg cctgtgcaag
    18001 tgggtgagga aatcagttcc agctcaacag ctcaaattat ccgcgctctc tatgagacag
    18061 gatttttcca gtccgtttcg cttgaacgcc aagggaatgt gttggtggtc aatgtgatag
    18121 agcgagcaac tattggctct attaccgttg taggaaataa ggaaataccc tctgataaaa
    18181 tgaaggcttt tcttaaagaa atgggtctgg tcaaaggcag agtatttcaa agatcttcct
    18241 tggagcgttt ggagaaggag ctgaaacagg cctacacagc cagagggaag tataattctc
    18301 gcattgaaac taaagtaact cctcttactg aaaatagagt agccattagt attactgtat
    18361 cagaaggtcg agtttcacgg attaaagaaa taaaaataat tggtaaccat gattttaaag
    18421 caaatgagtt attgcctgaa ttgacgttaa gtacaagcaa tctgtttact tattttacta
    18481 aaaaagatca atattccaaa ccaggaatgg atgcttcttt agaagcatta cgttcatttt
    18541 atttagatag gggatattta aaatttaatg ttgtctcttc gcaagttttg ctatcgcctg
    18601 ataaaaaaga cgtctatatc aatattcata tagaagaagg acctcaatat catttctcag
    18661 gttatgatgt ggttggaaaa acgatattac ctaaagaaaa aattgattca ctgatccagg
    18721 ttaagaaagg cgatgttttt tctcgtaaaa aggttactga atccatctct gcaatagggt
    18781 tagctttagg ggatgtagga tatggttttc ccgcgattaa tgccgaacct cgaatagatg
    18841 aaaataataa aacagtattt attacttttg tagtgcagcc gggccgtcat gtttatgtga
    18901 gacgtattaa ttttcatggt aatacgaaga caggagatta tgttctacgt aatgtgattc
    18961 gtcaggatga aggtgggcta ctgtctttac ataacatcaa ggaatctgag cgtcagttac
    19021 gaatgctggg ttatctaaaa aatattgatg tcaaaacgac gcctgttcca ggaaccaata
    19081 atcaggtcga tctggatgtt aatgtggaag aagctccttc agcagaagca agcgcatcca
    19141 tgggatatgg tacaaacggt tatcaattta atgcatccgt taaccaacgc aattttatgg
    19201 gaagtgggcg ttccatgggg gctgctttca atgcaagtca atgggggcaa gactactctt
    19261 ttaactatta taatccgttc tataccgata ctggggtagg ccggggagga agcttatatt
    19321 attcaaggat tgatcctaaa aatttaaatg tcagtacata cagttctaat cgctatggtg
    19381 gcgacatcag ctataatttc ccgttaggtg agaaaagcag ttttcaattg ggatatggtt
    19441 atcaagatat caatattaag tcagtgggtt acgtactccc cataatcaac ttcgttgctt
    19501 taaacggtaa tcattttcag gaaatcaggt tgacatccgg ttggagtaga aacagctatg
    19561 atcaaatgcc ttatcctaac caaggattta atcaacaagc tattgctatg gtcgcattgc
    19621 cagcaacatc gcaatctcta tcttactata agagctctta tcaggcgcat ttatattatc
    19681 ctctaacccg cggctggatt ttttctgtcc tgggtaatgt ggggtacgga aatacttttg
    19741 ataatttcgg attacccttt tttgaaaact actatgccgg tggtccagtg caaccaggcc
    19801 aggttcgagg gtacgatagt tattctttag gcccgcaaga taattttgga aatgcaatgg
    19861 gtgcgaattt ccttgtgaat ggaagtgtag gcctgatact accataccca ttgagtcgag
    19921 ataatgtaag aacgactatt tttgctgatg cgggtaatgt atttgcttcc gggacacctc
    19981 ctgctttacg tggaactccg gcaggtccga tgcgttattc agctggtgtg tcattagaat
    20041 ggcgctcacc ttttggtcca ttgtctttta gcttggctaa ggcattgaat ccacagccat
    20101 tggatcagac tcaactgttc caatttgctc tttcctcagg tttttagagg tattatggat
    20161 tagagtaagc ggattttagt attttaaaaa ctagtgctgt acatatcgca acctttttcg
    20221 aattgtgcta gcatttcaaa ctttaaatta ggagatcatt atgaaacgtc tgggtgcggt
    20281 gttagtcgca ttatttttgg gtttgttcgg cacgactgtg tttgctgatt gggcaaagat
    20341 aggtgtagtt gatttacaaa aaatcatgca aacctcaaat caaatgaagg aaatccaaca
    20401 gaagttggaa aaagaattta agcctcgtcg agacaaactt gtcgcaatgg aagcaagttt
    20461 gaaaagcgac atggaaaaat ttaagcgtga tagtgctata atgagcgcga gccaaaagaa
    20521 agaattggaa aagaaaattg ttgcgtcaca acaacagttt gaacgtgatg gacaacaata
    20581 tcagcaagaa ttgagcacgg ctcacaatga agctatggaa ggtttgtata ataaggttcg
    20641 tactgccatt accaaaattg ccaaggatga gaagtacgat attattgttc agaaagatgc
    20701 agcgcccttc agcagcgaat ctcttgatgt aacagataaa gttatcaaag caattaatta
    20761 atttggcgga tgcgttgcta accttagagg aactggcaaa ctatttaaat ggcgtctggc
    20821 atggcaatgc caatcacgcc atttttagtc tatcctcgtt ggctcgagct tcatctaagg
    20881 atcttgcgta ttttgataat cccttattgc agtctgtctt aaactctacc agagcaggtg
    20941 ctgttttatt aaagcccgag cacaaagatt ggtgtcctgt gaactgtatt gtagttccca
    21001 atccgtcaga agcaatgttt aaagcagcaa agagcctgcc tcaccttgct accaatgttt
    21061 gcggaattca tcctacagct caaatccaca aatctgccca aataggtcaa tacgtatcag
    21121 ttggcgctaa cagcgtgatt ggtgaaaatg tacaattgga tgattacgta actattggtt
    21181 ctaatacgac cattgaatct tcagtactga ttggcagagg gagtcagctg ggttctggag
    21241 caataattca ttctggtaca gttttgggcc aatctgtaat tattgattct gggtgtattg
    21301 ttggtgctgc cccgtttaat tgctacaagg aacatggtgt ctggcaacaa gcaccaaact
    21361 ttggtggagt tgtaataggc caaagaactc aaatcggtgc aaatacagta attcacagag
    21421 gctccattgg agatacttat ttaggcgatg gagtctgtat agacagttta gtactcatcg
    21481 cgcatgatgt ttatataggg aataacactg ctattgcagg ttctgctgca attggtgctt
    21541 tagtgcaaat aggcagtgat tgtatcatag gtggggcaag ttgccttgca gcgaacattc
    21601 gtttgactaa tgatgtagta ataacaggaa tgtctacagt gacaaaatcc atcgctcgcc
    21661 ccgggattta ttcttcggga acgacggttc acgatcatcg caagtggcgt cgtaacacag
    21721 cacggttcag gcatttggat gactacatta caaagctgat aacaatagaa aaaaaattga
    21781 ataataacaa ctaattatgt tttatcaatt tgaagtaaca ttatttttta aatttttaat
    21841 tgattagaat gagctaaacc aagataatga gccattttgt aatgcaatag gcgattaatg
    21901 agaactaggg tatgaacgaa tcgatagata taaatcaaat ttttactttg ttaccacacc
    21961 gctacccatt tattctggtt gatagagtga tagattacaa agtaatggaa tatttgattg
    22021 caatcaaaaa tgtgactatt aatgagaact tctttaccgg gcattttcca ggaaatccta
    22081 ttatgcctgg cgtactaatg cttgaggcct tagcccaagc ttgcggtatt ttagccaatt
    22141 tgtctcgcca acccaaagaa gggtatgagt ttctgcatta ttttgccggt attgacaatg
    22201 ctcggttcaa gcaagtagtg attcccgggg atcagttgcg attagaggtg agactggctg
    22261 gacaaaaaag agatttttgg cgtatgcacg gagaagctta tattggagac aaattggctt
    22321 gttccgcaga cttattaagc gcagcaaagg aaattaaaaa gtgatagatg aaagagcaat
    22381 gattcaccca tcggcgaagc tggcaagcgg agtgtcaata ggccctggga ccgtgatagg
    22441 cgctgatgtt gagattggtg aaaatacctg gatagggccg catgtggtaa ttgaaggacc
    22501 tactgtaatt ggtaaaaaca ataagatctt tcaatttgct tccgtaggag atgagccaca
    22561 agatatcact tacaagggcg agccaactcg tcttgaaatc ggcgataata atgtaatcag
    22621 agaatattgc atgattagcc gaggtactgt caaaggtgga ggtgtcactc gcattggcaa
    22681 tagcaattat ttaatggctt attcacatat tggccatgat tgtatggtag gcaatcatat
    22741 tattatggtc aattatgcag ccttatcagg tcatgtcaca attaatgatt acgcaattat
    22801 cggcccttat gctgcagttc accaattttg tcaggtggga gcctacgcct tcatagcccg
    22861 agctacctat gtcactaaag acgtattgcc ctatgttatg attgcaggtc ataccacttc
    22921 tgcttgtgga atcaacactg ttggtttaag aaggagaggt ttttcttctg ctgcgattga
    22981 ttgtttgcga cgcgcatata aaattatctt tcgcaaaggg ttaaccgttc aacaggcagt
    23041 ttcagaatta gagttgattc aaaatgagtg tccagaaatt attcctatga ttgatgcttt
    23101 gaatcaatcc acaagaggca ttgtgaggta agcaatggtt gttgcacctt acttggctgt
    23161 agctatcgga ggctctttag gcgcaatgtc tcgttattta gttacaataa tggctcaaaa
    23221 tgcctggggg attaaatttc cttatggtac tcttttagtg aacacactag gttcttttct
    23281 agctggtttt tttcttattg tattagtagg tcgttttagc gccgaggagt cgtttcgttt
    23341 attccttttt acgggatttt taggagcttt tactaccttt tcgagtttcg ctgcagaatc
    23401 tttatttatg tttgagcaag gatattggtt taaattaatt accaatatac tggtcaataa
    23461 tgttggctca ttgtccatgg tcttcattgg aacattagtt gctaaatatg tattattagg
    23521 acatcagggc tccaattaat aatttataga gtgttggctt atgttagata atcaattact
    23581 tcgagagaat cctcaatatg tagcaaccca attactgaaa cgaggatttc aatttgatgc
    23641 ggtcactttc agtcagttgg aagaaaagcg taaggcattg caagtaagta cgcaatcatt
    23701 acagaacgaa cgaaatttgc gttcgaaagc catcggtgaa gcaaaatctc gaggtgaaaa
    23761 catagagcct atgcgcgaag aagtgaataa acttggcgct aaactggagc aacaaaagac
    23821 tgaactggac gaggttctta agcaaattga ggtaattagc ctatcattac ctaatattcc
    23881 tcatgagtca gtacccgttg gtaaagatga attggataat caggaaatca gaaaatgggg
    23941 cgacgtacct gctttttctt tccctgtaaa gtcacatgat gaattgggtg aagcactagg
    24001 gcaaatggat tttgcgcttg cagctaagat tacgggcagc cgttttgtcg tcatgaaagg
    24061 gcatctggcc cgattacatc gggctttaat tcaattcatg ctggatatac atattcagca
    24121 acatggttac caggaaatat atgtacctta tattgttaat gcggatagct tactagggac
    24181 tggacagtta ccaaagtttg aggctgattt atttaaatta acaggagata atgggtacta
    24241 cttgacgtct acttcagaaa ttccagtgac gaatacagta agggaaatga tattgtctgc
    24301 tgagcaatta cctattcgtt atgtttgcca ttccccgtgt tttcgtagtg aggcgggttc
    24361 ttacggcaaa gacacaaagg gaatgattag acagcatcaa tttgagaaag ttgaactggt
    24421 ctggattacc aaaccggaag attcttataa tgctttagag caacttactc agcatgctga
    24481 agttatttta cagcgtttga acttgcctta ccgagtggtt gctttgtgta ccggggatat
    24541 aggcgccggt tcagccaaaa cctatgatct tgaggtttgg ttgcctagcc agaatactta
    24601 tcgagaaata tcctcttgtt ccaatatgga ggcatttcag gcacgccgaa tgaaagcgcg
    24661 tttccgcaat ccagatacta atgagattca attagtccac actctaaatg gatcaggttt
    24721 agctgttgga agaactttag ttgccattat ggagaattat caagatgagc atggaaacat
    24781 tcatataccg gatgctttga agccttattt aggtggtatt gatatcatct ctgtgaaata
    24841 ggtttgctca taattatgga tattttttct taaaagttag tcttcaatag gaaagagcga
    24901 tagataggct atattgagcc aaatcaattg ctcttaagta tttttaagaa aaaaatccag
    24961 tgactctaaa ctaatccaaa tgaaacattt tgatttttcc ttaagaatct ggccaaaaac
    25021 catagatacc aattattctg aatcttaagt atcacatttt agtctatggt tcatgaaaca
    25081 ttctggagtc aagaatccaa tgaacgaggt aattattcgt gagataacat ctgaagataa
    25141 agagatgttt ctttgtgcta tgcaaagtag tcatgcatta catcatcctt gggttaaggc
    25201 accgataacg cctgaagagt ttgatgagta tttttctcgc tatcaaaaac caaaccaaaa
    25261 aagctatctg cttctttctg acaataactt agcaggtgtc tttaatatca gtgaaatagt
    25321 acgtggttac tttcagaatg cgtatttggg cttttatgct gttgcagctt atgcgggtaa
    25381 aggatatatg agtactggtt tgaaattaat attaaccaaa gtttttaagg acatagggtt
    25441 gcatcgcttg gaggcgaata tacaacctga aaatacccgt tcaatctggc ttgtaaagaa
    25501 aaatggtttt cgctatgaag gattttcccc acgatatttg agagtgaatg gcgtgtggca
    25561 aggtcatgag cattgggcta tgacttatga agattttatc aaggataata atgaagcgct
    25621 tgaaaaagat catatcaata tagttgcata caatacggag tggccccttc ttgccaaggc
    25681 agaaatggtg aagttacgcg cctcatttcc cgcaaatagt gttattgata tccagcatgt
    25741 aggcagtaca gcaatacctg gcatggcatc aaaacccatt attgatattc agattgcagt
    25801 caggtcattg gaggaaatga agattattgc tgtgccgata ttacagaaat tagcttatga
    25861 atattgggaa gataatccag atcatgaacg aatgtttttt gtaaaaggca tgccacccta
    25921 tggtaatgga cgaacgcacc atgttcatat cgtcgaaaca tcatccaggc attggaaagg
    25981 aaaaatattt tttagagact atttactctc tcatccagaa gcagcagaaa aatatcagca
    26041 actaaaaatc aaactggctc aggaacacaa atatgatagg gagcaataca ctgatgcaaa
    26101 aagtgaattt atcaaccaaa ttttaaaatt agctcaaaat taataaatta tcatgggcag
    26161 acaaggataa atgggtgtaa cgaacttgga ttttttggcc cagggttaga tctaaacgat
    26221 gcatcaacgt ttttagaagt cctaatctta atgttgtgat ctacaaacat tgtgatctat
    26281 aattgtacta aatcacttta gttgagaaac gcaatggcag atcaacctcc aaaatccaaa
    26341 tggcatgaaa ttaaacgtct atcagaaggt gataataaag tagataacaa tcaagcacca
    26401 gtgtcaacgg cggatagaat taaaaagctt tatctggaca cacaggtagg cagtccaatt
    26461 gatactgttg aagaagcgga tgcccagcga aataatttag ggcctaaaga tactaaaata
    26521 gaaaattcct cccctgagaa tgatgagcca aaccctgata taccaacccc cggatgatag
    26581 cagaaattaa atacattgtt taaataaaat ggtttttgct gtttaacgat accattctgg
    26641 ttcggcaagc gcatgtgttg gttttgtgtt gacacccata ataaatactt gtaaattttc
    26701 ctacaagaaa atccttagtg ttcagtattc ttttagtcat cctgattgaa cacgcgatta
    26761 ggtttttggt gatggcttct ttatttttta ttaaagaggt cataaccgtg tcagataaac
    26821 cagattcccc atatatatta agcctgcaag cagacggtaa tttatttcct agcattttac
    26881 aatcaaattt agccatgaca ttggtttgga gttgttatgg acaatttatt gaatgaaaaa
    26941 aaattaacaa tttttacatc taatgaaatc caagataatt tgaaccgggt tctgaaaaac
    27001 tatgaacaaa atggcattat tggacttaat ggatatctca atcaggcaaa ccgaataagt
    27061 ttgcttaatg aacttgagat aatacaaaag gatgctgagc aggatattgt caggtattgg
    27121 aataataaaa tcattcattt ttattccaaa gatcctttga aacctgagtc agtggataga
    27181 gaatatgtca cagaacctta ttttcaagct tcaagtaata aggcacatgt attttatgaa
    27241 gtgattgata atatgcgggt agtcaatcga attggccatg gtatgcatct catcaatagg
    27301 tatccgatga tgcagcgagc agtttatgat agtcctgtac ttctggattt gctaaaaggg
    27361 attggattta agaagccaat ctgccatctc agtgtctata ttcccaaata tcctaatgga
    27421 ataggcagtg aggtaaaacc tcatcaggag tcaacatttg cctttactga accacaaagt
    27481 gtcgttgttc tgtgggtcgc cctggaagac gcgttaattg agaatgcctg tatgtatggg
    27541 gttttaggaa gtaatcattg gccattgaaa tgggtatcaa aagtggaccg agagacaaaa
    27601 acgcgtcact ttgagcaagt tcatcagtta catatccctg attttatgac agagcgcgaa
    27661 ttttatactg ccttggaagt gaaagccgga gatgctttgt tatttcacgg taattttgtg
    27721 cactgcagcc caatgaatac gtctaaaaac tcaagaaaag cattgtcgtt tcagtttatt
    27781 gaaacgtatg aagtcgatta tccggaaact aattggttat accctcctaa caaagtgtat
    27841 ttatattaaa aattaaattt gataagcgat tgaggaatca ctacatgtct atgtatgata
    27901 agcgccagtt ttttaaaacc tatttttcta gcgcaagcga gaagtcaatc ctggtaaagc
    27961 tgttggataa acgatttcat cctccctttc acactgcgaa catattggat ttgggttgcc
    28021 atgatggcgc attgatgaaa aatatagtga atgcgtacgc aaagcgttta ccggaaaagt
    28081 tagttattac cggagtggat cctagtttgc aagcaattaa agaatatgca aaagcagatt
    28141 ttccaattcc agtccaaacg aatacttttg ctggaactgc agaagattat tttgatgtgt
    28201 gcactgacca ttttgattgg atttttgcat cgcagtgttt gtattggtcc cctgatttgc
    28261 caaaaataat taagcagatt catgaagcag gggactcggc tttaattgtt atcagaggat
    28321 gtcatggaat ttatgaaatt caatcgcaat tcaggcatta tattgggaat catttagaga
    28381 aattttatac tgataaagaa gttgagatgg ccttaaggac ccaaaaaatt ttatttcaaa
    28441 aagaatctct aagtactttt atccggttgc ctgataaaga cagcattgaa ttgaaatggc
    28501 tgattttgtt ttttttgcaa atggaagagg aatcgttggg tgataaaata ttttgcgaag
    28561 tgcgggattg gattacctct cgagcgtcag gaagcattca tcatgaagtt catttttttt
    28621 ggttagggaa tgccattttt acaggagatt acaataggaa tttcccagtc aaatcgaaaa
    28681 aaaattctag gggataaagg caatgttttc aatgaaattg atttcatcat cagtcagcct
    28741 gaactgatgg atggcaagat cttctttcat atgatccaaa tttgtcgtgc cagttaaagg
    28801 caacattcct atctgttgtg aaaatctaaa aatgatttgc tcaatcgatt tctcgtactt
    28861 tttgctgaga ttctcaagtt tcggatgcct taactcatat tgattggccg ttaacagaga
    28921 aaacccctga taaataattc catgggcctg gcaatagtta cgaatttgtt tatcccataa
    28981 tagcctggca aagcatctat tttgaacaaa cttgggtttt accgaagcaa atgaaatgag
    29041 ttcgattagc tgttcataac tcacattgga tatgcccagg tgttttactt tcccatcttt
    29101 atgcagggat tccattttgt tccagacttc tttatcttca tcagaaaaat gatattgata
    29161 ataagggcca tgcaaaatat aggaatttaa ataatcagta tttaaatgtt caaggctgct
    29221 tgcaaaagat tgctggactt gaatagtcag gcttgctgta tcatcgtaag gtttgcgatg
    29281 atcctggcca tgagcatagg tgaacttgga ttgcaaaaaa atatcatcac gcaagatacc
    29341 tgatttttta tagtattgtt gcaaggcata tccaacgcct tcttcataat agtgttttct
    29401 ttggtttgca gtatctatac cccgaaatcc ggtagttaat gcaagctcga ccagttcgct
    29461 tgtacgcttt tctttccagg cggttccata tataaaagtt ggaaagccaa ggtccatcgt
    29521 tca
//
LOCUS       DACTJE010000021        29359 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.021, whole genome shotgun sequence.
ACCESSION   DACTJE010000021 DACTJE010000000
VERSION     DACTJE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 29359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29359
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.021"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            324..3012
                     /locus_tag="I9760_000717"
                     /pseudo
     CDS             324..3012
                     /locus_tag="I9760_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
     gene            2999..4258
                     /locus_tag="I9760_000718"
     CDS             2999..4258
                     /locus_tag="I9760_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAT5042505.1"
                     /translation="MKHFSAKKMIWTILLGFCCFAGPVSYAKTPPALSQLINEATQNN
                     PQIRAARDRLLAAIHVIPQAKALPDPKLNAGYINMSENIPMDVDPRREQMLGGQQEIP
                     FPGKLIVRGRIATLEAKRAEAEYQATCFAVIAELKRLYYDLYFVNKSIEIVQRNQELL
                     HEMEKSSEVNYSVGKTPQQDIYRAQTEISRLLMRLVILKQQRGSLQADINRLLNRSLE
                     ITIHTPAVLPVTPMGHNLEYFYTLVKSRAPQLIMQQRAVQKGRQAIRLSKMEYFPDVE
                     IEGGRLHDTGMHTKGYQVLLKATVPLYFMQKQNHAVRESLARYNADIEDLQTTYRMLS
                     FQVKNAYLLAERSAKLIHLIQHTIIPQASLTFTSSQATYGVGKVDFLTMLNNLLTLQE
                     NELEWHGELAEHEKAIAQIEESTGTFL"
     gene            4255..5388
                     /locus_tag="I9760_000719"
     CDS             4255..5388
                     /locus_tag="I9760_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAT5042506.1"
                     /translation="MNFKMIIKGVVILSLLSLLQVTQAQQAMDNHGKMDMKQSTQGNE
                     IVIDPARLQAIGITSEPVRRQVVEKIIRTVGRVEADERLVAHIHVRFDGWIENLFINF
                     TGEKVKADQALFTVYSPELVATQQEYLLALNAQKILSKNTTSSAARGAQSAFQAAHQR
                     LLLWGISEKDIEQLKRMGKITRTMTIHSPIQGTVLKKMALVGMRIEPGDELYTIADLS
                     RLWVLGDIYEYELPYIRLGQMADLTLTYLPNELFKAKLDFIYPTIDMKTRTAKVRFEV
                     DNQKEQLKPGMYVNLELKVPLGERLVVPKNAVLLTGERAVVFIYHGNGKIEWRDVTLG
                     VRAGDLLEIIKGVKEGDQIITSANFLIDSESQLKAAMGGMQHQ"
     gene            5463..8606
                     /locus_tag="I9760_000720"
     CDS             5463..8606
                     /locus_tag="I9760_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAT5042507.1"
                     /translation="MVERIIEFCARNRFIVLLFVLGFSFMGYIALKNTRMDALPDISD
                     TQVIVYTTWMGRSPDLIEDQVTYPIVTALLSAPKVVAVRGFSDFGFSYVYIIFEDGTD
                     IYWARSRVLEYMSQLSGKLPEGVTPQLGPDATPVGWIYQYALVDDNGKHNLAELRTFQ
                     DWYLRYWLRAVPGVSEVASVGGFVKQYQVNLDPVKVLAYNLSVPEIVEKIRMSNNDTG
                     GRVIEFSGVEYMIRGRGYIKSTEDIEKIAVGTNANGTPILLRDVATVQLGSDMRRGVV
                     DLNGQGEAVGGIVIMRFGEDVLQVIGRIKDKLKELASAIPEGIKMVTVYDRSNLIREA
                     ISTANWNLIEELVVVGLLIIVFLGHFRSALIPIITLPLAILISFIPIYFLHVGLNIMS
                     IGGIIVAIGDMVDAAIIMVDNAHKRLADWEAKGSPGDRTQVLIDSAKEVGPAIFSSLL
                     VIAISFMPVFTLEAQEGRLFSPLAYTKNLAIFMSALLAITLIPVLLPLLVRGRIIPEQ
                     KHPITRWMQAMYAPVLRLALRYRQFVVGIAIVLAMATIPLYKLIGSEFMPPLYEGTIL
                     YMPVTLPGISVTQATALLQEMDKKLKAFPEVAHVFGKTGRAETSTDPSPFNMMEVIVE
                     LKPKEFWRKGVTYESLVKEMDEALQFPGVSNAWTMPIKARNDMLTTGIRTAVGIKIFG
                     PDIKKIEAIGKEIEMEAKEVKGTSTVYAERVAGGYFLDFQINRDQLARYGLTIMDVNR
                     IIESAVGGENIATTIEGRERYPVNVRYLRELRDDPDKLNRILVKTPSGAEVPVAQLVT
                     LTFRSGPAMVRDENGMLAGYVFIDISGRDIGGYVEELKQVVNAKVKLPPGYTLAWSGQ
                     YEFMQRVYERLKIFVPLTLAIIFVLFYFTFRTITETLMVMLGVPFALFGGFLLLYLLG
                     YNMSIAVWVGMIALAGVAAETSAVMLAYLDSDYNEQKEKGLLKTLPDLIQLVQLCAVS
                     RIRPMAMAGLANIIGLLPVMWATGIGADVMKRLAAPMVGGVFSALLLTLIVIPVVYVM
                     WRWQVDLKKTADLTETR"
     gene            8603..9964
                     /locus_tag="I9760_000721"
     CDS             8603..9964
                     /locus_tag="I9760_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT5042508.1"
                     /translation="MKLTFLGATETVTGSKYLLTIGSKKILIDCGLFQGYKELRLRNW
                     APLPIDPHDIDAVIITHAHIDHTGYLPLLVKNGFQGKIYATPGTKALCSILLPDSGHL
                     QEEEARLANKYGFSKHQPALPLYTEKEARAALQYFETIDFDTPHQLFHDFSFEYHRAG
                     HIVGAAMVKIKTQKGSIVFTGDIGRPYDPVMKAPAFIQETDYLVMESTYGDRLHDATD
                     PLPQMAQVINQTVKRGGSVVIPAFAVGRAQSLLYFIYELKRQGQIPKDLPVFLDSPMA
                     IDATHLLCTYKEDHHLTAEQCRGLCRVATYVNTPEESKEIDRHHMPQIIIAASGMAQG
                     GRILHHLKAFAPNPKNTLLFTGFQAGGTRGARIVNGEKQVKIHGSFIPIQAKVVVMSS
                     TSAHADYLELLEWLTHLTNPPKKVFITHGEPHSAQSLKQKIEKQLGFSCTIPSYLQTE
                     ELW"
     gene            9961..11475
                     /locus_tag="I9760_000722"
     CDS             9961..11475
                     /locus_tag="I9760_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HAT5042509.1"
                     /translation="MSKQTSHGLRLKHLGIKTYHEAIIYMREDCHVCHSEGFEVQTRI
                     QVTLGRRSIIATLNVVTSELLQPGEAGLSDYAWDSLYAKEGDEIQVSHPKPLESLSYV
                     HTKIYGNELSFEQMKVIIDDVLSGRLSDVQISAFLAASSAGRLTRTEIMKLTKAMIDS
                     GDRLSWSSPLVVDKHCVGGLPGNRTTLIVVPIVAAFGLMIPKTSSRAITSPAGTADTM
                     ETLAPVHLSPQKMRQVVEQENGCIVWGGAVSLSPADDVLIRVERAIDLDSEGQLVASI
                     LSKKIATGATHAVIDIPVGPTAKVRNQSMAFLLKQLLEEVGNELGLVVHTILTDGSQP
                     VGHGIGPSLEARDVMAVLQGLPDAPNDLRERALTLAGAALEYSSKVQPGLGKSIAKQL
                     LESGQAFKKFQAICEAQGGMRELTKARFTHPVVAAKAGKVSLIDNRKLAKIAKLAGAP
                     KSKSAGIDLHVHLNKSVEKGEPLFTIHSESSGELNYACDLLRDKQDIIILGENS"
     gene            11472..12377
                     /locus_tag="I9760_000723"
     CDS             11472..12377
                     /locus_tag="I9760_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAT5042510.1"
                     /translation="MKPLLFSLFDSSEIARQLQEILQVEAGKVTFHRFPDTEWYLKID
                     SEVNNRSIIVVDSLNQPDKKILPLLFFAKTAKELGAQKIGLIAPYLSHLRQDKRFHEG
                     EGITSRYFADILSTSLDWLMTIDPHLHRYHSLDEIYSIPTFVLHATSMIATWIKHHVP
                     KPILIGPDMESEQWVADIAEKSSFPYAILEKIRRGDKEVAISMPFIPELESSTPVLVD
                     DIISTARTMIETVKQLQQTGVKSVLCIGVHALFAKEAYSLLSGIKGVQTITCNTIRHA
                     TNTVDVSDLIVEALVKNKFAKAIKK"
     gene            12649..14865
                     /locus_tag="I9760_000724"
     CDS             12649..14865
                     /locus_tag="I9760_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAT5042511.1"
                     /translation="MRHDHHQGHTHTDKEPTCCHKEHGLHKRQDTASKTDGTVIYTCP
                     MHPEVRQSNPGICPICGMTLEPESITTEEGASPEYLDMRWRFWVGLILTLPIVVLEMG
                     THGFQHFISANFSNWIQLLLATPVVLWGGWPFFVRGWHSLKTRQLNMFTLIAMGIGVA
                     WAYSMVAVLFPGIFPLAFRSQFGTVAVYFEAAAVITTLVLLGQVLELKAREQTGSAIR
                     ALLKLAPESAHRIKEDGGEEEVSLDKVHVGDKLRVRPGEKIPVDGEVLEGRSFIDESM
                     VTGEPIPVTKEAGAKVIGATINQTGSFIMKAMHVGSDTMLARIVQMVSEAQRSRAPIQ
                     RLADTVSGWFVPAVILVAVLSFILWALFGPQPAFSYGLIAAVSVLIIACPCALGLATP
                     MSIMVGVGKGAQSGVLIKNAESLERMEKVNTLVVDKTGTLTEGRPKLTRIVTDDEFDE
                     EAILTFAASIEHQSEHPLATAIVMAAKEKQLSLGRVEDFEAPTGKGVIGKVNGYRIAV
                     GNARLMQEYGSDKLALFEKADELRSKGASVMFMAVDGKTVALLVVEDPIKSTTPETIR
                     ELQQSGIEMVMLTGDSKKTAEAVADTLGIKKVVAEIMPQDKSRIVSELRDKGLVVAMA
                     GDGVNDAPALAKADIGIAMGTGTDVAIESAGITLLHGDLRGIAKARRLSEITMSNIRQ
                     NLFFAFIYNVLGVPLAAGVLYPITGLLLSPIIAAAAMALSSVSVIVNALRLRRVRL"
     gene            14956..15837
                     /locus_tag="I9760_000725"
     CDS             14956..15837
                     /locus_tag="I9760_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT5042512.1"
                     /translation="MVFPVIMIVVGIVFLIGGAYLIKSVLQQTIHYRWQWLVYLVFIG
                     AFILSYSASIFYGLLVDPHCLNYFYGSVFLLGATYVYLSCRLMTKTIQKMEVMDDLKN
                     RYELLRHHVKYDSLTGCHNRETLFEMLNTRFQQITSNDGWAIVLFIDMDKFKAVNDRY
                     GHDVGDNTLAAFGQLLRQRLRKDDIVARYGGDEFIAVMEHISLDEAKKIAENLITVAK
                     QSFKKGILSKLNLGCSIGITQMNPHSASVNSVIKEADLACYAAKQRKINGSVCVYNPM
                     IATRNDTIDLGIHHVAE"
     gene            16008..16379
                     /locus_tag="I9760_000726"
     CDS             16008..16379
                     /locus_tag="I9760_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAT5042513.1"
                     /translation="MIHFQMKRTILLMVSALLVGCSYNAKLTPEGEKVTLLQSGKPSK
                     SCVFLGEVRASDRNGMSQSYQSHEHLIQDELNILRNEAALLGANTVLVTQHQQTYEGN
                     PKNDFVADHWMVGRAYRCKTN"
     gene            16404..17717
                     /locus_tag="I9760_000727"
     CDS             16404..17717
                     /locus_tag="I9760_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAT5042514.1"
                     /translation="MENNSMRWFSCWVALLLLYSSLVTAGNVLTLQEITQIALANNKD
                     LKAARYTVSIAKARLIQAGLWPNPSLNLSNNDDRLFNDEGEYSRSAGFSQAFPISGRI
                     AKQKTVAHLDVLKAMAEIREAERQLSSKVANAYYAAVITERRMQQVNYLLRINRELVD
                     VIHNRYHAAEISKLDENAARIEYLRIGQEKHLLHSFSISQYALLNQLMGREANYPLSL
                     KRDVIAGKKLPELAFLKTLALKNRPDRQAIALSIHRANADRRLAKAERFADWTVGLGV
                     QQDKIVVEEGPPQPADRTLGVTLSVPLPLLNRNQGRILEASHTGTQAVMALRALDLVI
                     ETEIASNYAQLKALQMSLRETQTVSLKLAVENVKLARDSYESGQISFLNVLQVQKQQN
                     DLQTTYLNTLEKYLQVYVALCTAIGPGKTNGFCPYLAYQRNGDSK"
     gene            17701..18684
                     /locus_tag="I9760_000728"
     CDS             17701..18684
                     /locus_tag="I9760_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAT5042515.1"
                     /translation="MGIVNERWQQMRRMALISLFLSLFFPIQGCFAHGEEIEVSEGGT
                     KGPVHLTPAQTKMLDIKVAEATNRPMAQLLGLNGQIQLLPDAQADVSIRISGSVTAID
                     VNLGDSVKAGQRLATVQSRLVGNPPPSVAVTAPIAGIIDARNINLGQAVEPNTVLFHI
                     SNRDKLLVVAQVYEEDLGKVKVGQEVTIHALSYPKRTFPGQVTLIEPNLDPLTRTANV
                     RITLDNKEGLLKTGMFVRANVILARNEAALAIPNAALLQADNEQFVFVQNGDTYERVD
                     VQVGAVDDDFSEITDGLVPGDLVVTQGNRELYTLWLSGGSKFQSGSHEEPQ"
     gene            18685..21861
                     /locus_tag="I9760_000729"
     CDS             18685..21861
                     /locus_tag="I9760_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAT5042516.1"
                     /translation="MFTRLIAWSLHNRPLILALTIILCLLGGYTLKRMPVDVFPEFAP
                     PQVVVQTQAPGMATQDVETLVTYPLESAINGTPGVASVRSKTSVGLSTITVVFDDKTD
                     IYRNRQLVNERIQQVVNRLPPGVSSPVMLPVTSAVGWLLKYALVSRTESPETLRTISD
                     WTIRPRLLALGGVASVVSLGGEVKQYQVQLIPSRMLAYGITVEEVRQALTTGNQNVPG
                     AFVHQAGTEFVVSTIGRIKTLKDIKKTLIVVRNGVPITIDNVAAVAFGGEIKRGDASY
                     NAEHAVIGTVSKIYGADTLTTTHKVEQALADIKKSLPAGIELITEVFRQANFIESAIH
                     NLTKALIEGAIIVIAVLFLFLMNWRASFITFLSMPVSFIVGILVLHYFGFGINSMTLG
                     GMAIAIGEVVDDGIITVENVVHRLRLNRMSDKPLPAIEIVFDAVLEIRSSVVYATIII
                     SLVFLPIFFLSGIPERIFSPLAIAYIASVLGSLVVSITMVPALCYLLLVRKSEKQQDN
                     KVVMHALSQEERYHLAEEEAAKQSQAETRFVIWLKKHFLMGLNWSLDHCKTVVTIAIG
                     AFVFALALLPFFGTSFLPEFHEGNFIIVMSTLPGTSLDESMRLGNQVSKTLLKYPQVI
                     SIAQRAGRSELDEDALPPNISEFDVRLDFGKDKSISPDELLQRIRADLANIPGALFNV
                     GQFIAHRMDEVLSGVRAQVAVKLFGDNLATLNELGQSVEVLLKTVPGIVDVNKEQQIN
                     VPQLVITIDREKATRYGINVGQISEDVQVLLNGVTVSSILEGQRTFDLYLRMSETGRD
                     SVKAIQNMLIDAHGTADQKTKIPLRAVAEIRLEPQPFAINRENVQRVLVIAFNVQERD
                     LGSVIQEVQQRVKEKVTLPSGYFIQYGGQFESQQQASRVIILFGALAIFIMLILLHKA
                     FGTFREALLVMFNLPLALIGGVVSLFLAGGNMSVAAMIGFITLFGIAARNGIILVSHY
                     NQLRVQGQPREQVVIQGTLDRLVPVLMTAATAALGLIPLLWGSPAGKELERPLAQVLL
                     GGLFTSTVLNMFVVPTVYNAIEVWRELQ"
     gene            21883..22335
                     /locus_tag="I9760_000730"
     CDS             21883..22335
                     /locus_tag="I9760_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAT5042517.1"
                     /translation="MKHVTLKQKSHFWLGLITSVLLVCGYSQAMADSTEESTQSIPKT
                     IPAIWEAVDKHAASINQVLSSDDLTTIHQHAFAIRDLVNALPALSKDLSEEQKKTLQQ
                     NLSYVSQLATRLDKSGDANDKKGTQVNWEKLQKVLAQLRSLYPSGVTN"
     gene            complement(22478..22789)
                     /locus_tag="I9760_000731"
     CDS             complement(22478..22789)
                     /locus_tag="I9760_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042518.1"
                     /translation="MKKINFLLYLLTMLAISFVTLGHGTTEATDTTNQTKHLYRGSSI
                     SLEELAQVADLSSARDQKGNPVTLEEESYQVGSFTKGTVTIIPKSGKADDVITLDLKP
                     N"
     gene            23026..24705
                     /locus_tag="I9760_000732"
     CDS             23026..24705
                     /locus_tag="I9760_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HAT5042519.1"
                     /translation="MGKENTDSPYQMGHDFFSYQLDCWQRSILFWNTLRERANNMMEH
                     EQQGLPPLLNFKYEFVLDGKSLEPKTNYALVKILEVGDVCFEECFDPHAHPVIIVDPR
                     SGHGPGIGGFKRDSEVGIALHSGHPVYFVIFYPNPVSHQTLADVLMTLRHFVEKVQAW
                     HEGKAPILYGNCQAGWMLALLVSDCVGSVGLTVMNGSPVSYWSNSEEEANPMQLLGGL
                     LGGSWSARFLSDLKEGIFDGAWLVSNFELLNPTTAIWDKYYGLFDEIDGERERFLEFE
                     RWWNGFYQFSQEEIMATVNNLFIGNQLERGEMRIHKGCVYDLKRIQSPLVLFASQGDQ
                     ITPPRQALHWIRTIYPTTQALKKAKQRVVYLLHPSVGHLGIFVSAKVVRLHHRAILEH
                     SDAIEQLQPGLYEMIIVNPTGDPDCSKEQYHVRFEARELTELCTTSSTQPFDKVRQTS
                     EANDSIYQKVIQPSVQSLSNPFLSWWLEKTHPMRLSRYVFSEKVNPLMKAIELLSPPI
                     QANRRMATQSNCFKKIEHGVAQMMRDSLESSRIMRNEVMTNWFEALYKDPD"
     gene            25070..25597
                     /locus_tag="I9760_000733"
     CDS             25070..25597
                     /locus_tag="I9760_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HAT5042520.1"
                     /translation="MLVPQTGIRNASTMIEIIPNWHPIFVHFTVALFTVSVILYALIY
                     LASYTHWNTKPLIVELEIVARWCLWLAALSTVTTVSAGFYAFYTVKHGAMVHAVKVIH
                     RNWALGSASAILLMAFWVVWRYIKRQKPTLIFLMVLFLVQVLLLTTAWYGAELVYRYG
                     YGVLPVKAKQTVSPH"
     gene            25624..28710
                     /locus_tag="I9760_000734"
     CDS             25624..28710
                     /locus_tag="I9760_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HAT5042521.1"
                     /translation="MVHKDLRCSIRNYCFIFSFLLLSSSALFAQHEQHGASTQPLPSP
                     KTTPSQSKPAQYKMEQPIAKKQSVRTVKPLTMTGGAKRTVNLVVAYKTVNFAGKLRRA
                     IAVNGQIPAPTLHFKEGDDVTINVYNHLDEGTSIHWHGLLVPWQMDGVDEVSQKPIPP
                     GGVFHYRFKLYQRGTYWYHAHAKVQEQEGLYGTFIIDPPNPPSYHYTKDYVVVLSDWS
                     NTPAEQVLANLKKDGDYYSPRFPLQPSLMKFLHDYRNASPEERKKLIADYKMMQQMRM
                     SIYDLSDVAYDAYLLNGHPKSHPWTAPVKVGDTVRLRFIGAGASTIYRVKIPDAKVDM
                     VHIQGNDVDPYPIEDFWIAPGETYDILVTIQKNKPYIIYAESIDTLGKVYGALMTHPN
                     QVVNYQQVTPFPEPLPVTREMMANMMQSMNGGAMSGNQQHALMNKKTSSPAMKPSMTM
                     PSHSKTMSSQSANHSMSSMTAQKKMDNASNHSAHTASMKSKSSSTSMNKSMDMPGMNH
                     GAMSQSKTSNSAQMKSDSSSTSMNKSMAMPGMNHGAMSQSKTSNSAQIKSDSSSTSMN
                     KSMAMPDMNHSAMGQNKTSNSANDTSMNRNMKSDRPMEQGMSMNGSMNMKMPIEPTII
                     GDKIEPPDSAKATTLGTKYQELKGAVKTNNPNKPVDGIIKMELFGYMDRYIWFINGLP
                     EYKAKPILIEPGKRYRIIFTNNSMMRHPMHIHGHWFILRNGHGAYDPLLHTIEVPPGA
                     TAVADFDTEASGQWFFHCHHLLHMTAGMARVFQYTTIIEIANGTRKPDNYAYQQAYIN
                     RPVVREDEVMPLDASLIKHPAGHHQGFYRSSYIELGEDPFHNAQEMTFRGLYGPDYNK
                     LQLYTEDAEIYKGTVENADIDVFYWHLISQFWAVKGGVNYFYRPGGPYWQPGIGIEGL
                     MPWYIDTNIRTYYRDGSVKFDIQLARANQLTNNFFFLTGIRSILATHTVVKNEIGNGL
                     NQMRYILRPYYRLKPGLNIFSEYEHDVEYGALKRILRSQGEPTTQDTITLGVAVLF"
     gene            complement(28949..>29359)
                     /locus_tag="I9760_000735"
     CDS             complement(28949..>29359)
                     /locus_tag="I9760_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAT5042522.1"
                     /translation="GCGGLRQVNADAKNVNLYFSYPTGNCRFLGIIKNPNVHEVVDIR
                     TSLKDLEKDDNNFLKNEGAKLGANVIVLVTHTPSEAHKRYVRGSKNLTTINIHSVIAN
                     AYYCPHVKVDQLQQRNLEIKQTPLFENDDLQINH"
BASE COUNT         8018 a         5953 c         6938 g         8450 t
ORIGIN      
        1 tttaaaatca aggatagaaa ttatcatgaa acaaagtcat tggatggttt tggcgtgttg
       61 tttagtcctt actgcgtgca tgagcagtgg cacgagtagt aagtacacta aaagtctcga
      121 agagcatgtc gcccaaggcc atgttggcca tggtggaggt ggtggtcgct aacactaaaa
      181 tctttattac tcaatcctaa agattcatat gagcgctcat cctataaagt ctaagaacta
      241 ttaatctatt cgggcactaa aaattgagat aaaatgatgc gcaccacatc atagtggacg
      301 ggcgaatgca aaggatggct gtcatggcag aacaatggca cgaaaaagca cctgcagata
      361 tggtgttgtt gtttcaaacc gacttatctt taggactatc tgaaaaagta gcagaacagc
      421 ggctaaaaga agtaggacct aacctgctga gtcaacagaa aaaaacatcc cctttcgtca
      481 tttttttgca gcaatttgca agtgtggtga tttgggtact tttaggtgct gttgttgtgt
      541 cttttttgtt ggatgaaaaa gccgatgcca ttgccatttt ggccattgtc attttaaatg
      601 ccatcatcgg attttttttg gaatatcgcg ctgatagggc aatgcttgcg cttcagcaaa
      661 tggctgctcc caaagcaaca gtgcttcgcg atggccatgc gaaggtgata gccgcttctg
      721 atatcgtacc aggtgatatt cttctttttg aaagtggcga tttaattgct gccgatgctc
      781 gtctttttga attgtcagcg cttaaggtta atgaggcgcc actcacgggt gagtcgatac
      841 ccattgctaa aaatctggat gtttacactg ccgagacgcc tctagctgac cgtaaaaaca
      901 tggtgtttat gggcacctct atagccgatg gcaccggtcg cgcgcttgtt gtggcgactg
      961 gtatgcaaac ggaaatgggg cacattgcca aaatgctggg tgaggcatct cgtgatgaaa
     1021 cgcctttgca gaaaaaactc aatcaagtgg ggtctcgtct attgtggctt tgctttttca
     1081 ttatcattgc catttttggc ttaggtcttt tacgtaatat ctctctattc agcttattca
     1141 tgagttcagt cagtcttgcg gtggctgcaa ttcctgaagg cttgcctgct gttgtcacgg
     1201 tggctttagc gcttggggtg cagcgtatgg tacggcgtgc cgtactggta agacgattat
     1261 cggcggttga aacactcggc tgtttacaag tcatttgcac cgataagaca ggaacgttaa
     1321 ccgtaggtga aatgaccgca cgtcagcttg tgacagcaag tgatgtttat agcattcatg
     1381 gggaaggcta taacctctcg ggaggattta ctcttcaagg tcaagaaatt aatgtgtcag
     1441 aagaccagtt gttgcaagct actttgcaag cgatggtggc ttgcaataac gcgagttttg
     1501 agagccaaga tggacaggta gctactgtgg gagacccaac tgaagtggcg cttttggttg
     1561 ctgctgccaa aggaggcatt tggcaagggg aactgcaaac ttcttatccc cgtatcaaag
     1621 agttaccctt tagttctgaa cgcaaacgca tgacggttgt gtgtcgacaa gaccatgaac
     1681 acatcgcttt tgtaaaggga gcacccgaac tcattttgga gcgctgcacc catattttga
     1741 ccaaaacagg tattaaaaaa ttaacaccga atgataaggc gcgcatgaaa caatcctgtg
     1801 aactcatggc cagcgaagca ttgcgactgt tagcttttgc gaagcgccaa ttagagccta
     1861 cagctagaaa aagaagacga cgagattgaa cataacctgg tgtttttagg tcttattggt
     1921 cttcaggacc cacctcatgc aagtagcaag gaatccatca gtcgctgtaa aaaggctggt
     1981 attaaaccag tgatgattac aggagaccat cctgatacgg caagagccat tgccaaagag
     2041 cttggcattt tggaggcagg tgaccgattg ctcactggca atgaacttga aaacatgtcg
     2101 gaggaagaat ttaaccattg cgtcaaagac attgctgtct atgctcgtgt gactgcggag
     2161 cataaattaa aaatcgtacg ggcctggaaa aaacaacaaa tggtggtggc gatgacaggc
     2221 gatggggtga atgatgcgcc tgctttaaga gaagcttcgg tgggcattgc catggggaaa
     2281 acagggactg ctgtaactaa agaagcctct gacatcattg tcatggataa taacttcacc
     2341 tcgattgtgg caggtattga agaaggtcgc acgatttacg ataacattgc caaaacgctt
     2401 gcttatttat tagcaggcaa tagtggggag ctattagtcg tgtttgtagc acttttaatt
     2461 ggttggcccc tgccgttgct tcctatccaa ttattatgga ttaacttagt gacggatggt
     2521 ttgccggcca ttggcttagc taccgatatg tcagagccag ggattttgaa tcgtccaccc
     2581 agagccacgc aaaaatccat gatggatatg gcgtttttca aacgagtaac ctttgttggt
     2641 tgtttaacgg ctttagtaac tcttggtgtt ttcgcttatg aatatttaat cgataaggat
     2701 ttaatccaag ctcaggatgc agcgttttct gtcttagtaa ccgccgaact tttacgagcc
     2761 tttggagcac gaagcgacac gaagaacatc tggcaagtag gattgttttc caatttaaga
     2821 ctttttttta ttgtgagcat cagtttttcc ctgcaggtct tgattcatca tatcccggca
     2881 ctgcgcgaat tatttgggat acaaccggtc tcattcaccc aatgcctggt ttggattcta
     2941 ttaggcatgg tgcctttgtt agtgatagaa gctcagaaac ggctaaggaa ggcacccaat
     3001 gaagcatttt agtgctaaaa aaatgatttg gactattctc ttaggtttct gttgttttgc
     3061 agggccagta tcttatgcca agacaccgcc tgctttatcg cagctaatca atgaggccac
     3121 gcaaaacaac cctcaaattc gtgcggcacg cgaccgacta ctggcagcaa tccatgtgat
     3181 tccacaggca aaagcgttac ccgatccgaa gcttaatgca gggtacatca acatgtctga
     3241 gaatatccct atggatgtgg atccaagacg cgagcaaatg ttaggtggtc aacaagaaat
     3301 tccttttcct ggtaaactta ttgtacgagg gaggatagca acgctagagg ccaaaagagc
     3361 ggaagcagaa tatcaagcca cttgtttcgc tgtcattgct gaattaaaac ggctctatta
     3421 cgacctttat tttgtcaata agtccatcga gattgttcaa agaaaccagg agcttcttca
     3481 tgaaatggaa aagagttctg aagtcaatta cagtgtgggg aaaacaccac aacaagatat
     3541 ttatcgtgcc caaacggaga tttctcgtct gctgatgcgt ttagtcatct taaagcaaca
     3601 acggggatcc ctgcaggcgg atatcaatcg ccttttaaat cgttctttag aaattacaat
     3661 ccatacacca gcagtgctcc ctgtaacgcc aatgggtcat aatttagagt atttttatac
     3721 gctcgttaag agccgagcgc cgcaattaat catgcagcaa cgtgctgttc aaaaaggacg
     3781 acaagccatc cgcttaagta aaatggagta tttcccggat gtggaaatcg aagggggacg
     3841 acttcatgat acgggaatgc acaccaaagg ctatcaagtc ttgttaaaag caaccgtgcc
     3901 tctttatttc atgcaaaagc aaaatcatgc cgtgcgcgaa tcacttgcgc ggtataatgc
     3961 ggatattgag gatttgcaga ccacctatcg tatgctttct tttcaagtga aaaatgctta
     4021 tttgttggca gagcgctctg caaaactcat tcatctgatt caacatacca tcattccaca
     4081 agccagctta acatttactt catcgcaagc cacctacggc gtgggcaaag tggatttctt
     4141 aacgatgctg aataatttat tgacgctgca agaaaatgaa ttggaatggc acggtgaact
     4201 cgctgaacat gaaaaagcga ttgcacaaat cgaagaaagc acagggacgt tcttatgaat
     4261 tttaagatga taatcaaggg tgtggttatt ttgagtttac tttcgttgtt gcaggtaact
     4321 caggcgcaac aggccatgga taatcatggc aagatggaca tgaaacagtc cactcaagga
     4381 aatgagattg tgattgatcc ggctcgttta caagccattg gcattacgtc agagccggtg
     4441 cgtcgccaag tggttgaaaa gataatccgt actgtggggc gtgttgaagc cgatgagcgg
     4501 ctcgttgccc atattcatgt acgctttgat gggtggattg aaaacttatt cattaatttt
     4561 accggagaaa aagtcaaagc agaccaagct ctttttaccg tttacagtcc tgaattggtc
     4621 gccacccagc aagaatattt gctcgccctt aatgcccaaa aaatattaag caaaaatacg
     4681 acatcgagtg cggctcgtgg tgcgcagtct gcttttcaag cagctcacca aagattgttg
     4741 ctgtggggga tatccgaaaa agacattgag caattaaagc gtatgggcaa aattactcgg
     4801 acgatgacga ttcattcccc catccaaggt acggttctta aaaaaatggc gctggtgggc
     4861 atgcgcattg agccaggaga tgaactctat accattgctg atttatcacg cttgtgggtt
     4921 ttaggggaca tttacgaata tgagcttcct tatattcgtc ttggtcaaat ggctgatttg
     4981 acccttacct acttgcccaa tgaattattt aaggccaagc ttgattttat ctaccctacg
     5041 attgacatga aaacacgtac agccaaggtt cgctttgaag tggacaacca gaaggagcaa
     5101 ttaaaaccgg gcatgtatgt gaatctggaa ctcaaagtac ctttgggtga acgtcttgtg
     5161 gtccctaaaa atgctgttct actcacaggt gagcgcgcag tggtgtttat ttaccatggc
     5221 aatggcaaaa ttgaatggcg ggacgtgacc ttaggggtta gggcagggga tttgcttgag
     5281 ataatcaaag gggttaaaga gggcgatcaa attattacgt ctgccaattt cctcattgat
     5341 tcagagagcc agttgaaagc ggccatggga ggcatgcaac atcaatagat tttctatccc
     5401 actataatgg gaaagaagct ctaataaaga ggcgtgattt agccttggaa taaagggatt
     5461 ttatggtcga acggattatt gaattttgtg cacgcaaccg gtttattgtg ttgctttttg
     5521 tgctcgggtt ttctttcatg ggttatattg ccttgaagaa tacccgtatg gacgcattgc
     5581 ctgacatttc agacactcag gtcattgtgt atacgacctg gatgggacgt tcgcccgatc
     5641 tcatcgaaga tcaagtcacg tatcctattg taactgcatt gttgtcagca ccaaaagtag
     5701 tagctgtgcg gggattttct gattttggct tttcttatgt ctacatcatt tttgaggatg
     5761 gtacggatat ttattgggcg cgaagtcggg tcttagaata catgagtcag ctgtctggaa
     5821 aattgcccga aggagtgact cctcaattgg gccctgatgc gacaccggtg ggttggattt
     5881 atcaatacgc cttggtggat gacaatggca agcataacct ggctgagtta cgcacatttc
     5941 aggattggta tttgcgctat tggttaagag cggttcctgg ggtgtctgaa gtagccagtg
     6001 tgggaggttt tgtcaagcaa taccaagtca atttggatcc agtcaaagtc cttgcctaca
     6061 acctatcggt tcctgagatt gttgaaaaaa ttcgtatgag taataacgac accggtggtc
     6121 gagtcattga gttttcgggc gtggaataca tgattcgtgg tcgcgggtac attaagtcta
     6181 ctgaggatat tgaaaaaatt gccgtgggca ctaatgccaa tggcacgccg attttactgc
     6241 gtgatgtcgc tacagtacaa cttggctcgg atatgcgccg cggggtggtc gatttaaatg
     6301 gccaaggcga agccgtgggc ggtattgtca tcatgcgctt tggggaagac gtgctgcaag
     6361 taattggtcg catcaaggat aaattgaaag aattagcttc ggccatcccg gaaggtataa
     6421 aaatggttac tgtgtatgat cgcagtaatc tcattagaga agcgatttcc accgccaatt
     6481 ggaacttgat tgaagagctc gtcgtggttg ggttactcat tattgtgttt ttaggtcatt
     6541 ttcgttccgc actcattcct attattacct tgccactggc gattttgatt tcgtttatcc
     6601 ccatttattt cttacatgtt ggacttaata tcatgtccat tggtggcatt attgtcgcca
     6661 tcggtgatat ggtcgatgcg gccatcatca tggtcgataa tgcccataaa cgcttagcgg
     6721 attgggaagc aaaaggaagt ccaggcgatc gcacccaggt gttaatcgat tcggcgaaag
     6781 aagtagggcc tgcgattttt tcatcccttt tggtcattgc catctccttc atgcctgttt
     6841 ttaccttgga agctcaagaa gggcggctgt tttcgccctt ggcttatacc aagaacttgg
     6901 ccattttcat gagtgcctta ctcgccatca ccttaattcc tgtcctattg cctcttttgg
     6961 tgcgtggtcg aatcatccct gaacaaaaac atccgattac gcggtggatg caagccatgt
     7021 acgcgcccgt gttgaggctt gctttgagat atcgccaatt tgtggtggga attgccattg
     7081 tattggctat ggccaccatt cctctttata aactcattgg ctctgaattc atgccccctc
     7141 tttatgaagg gacgattttg tatatgccgg ttactttacc aggtatttca gtcactcaag
     7201 cgacggcttt acttcaagaa atggataaga aattaaaagc atttccagag gtggcgcacg
     7261 tttttggcaa gacgggaaga gccgaaacgt ccaccgaccc ttctccattt aatatgatgg
     7321 aagtgattgt ggagttaaag cctaaagaat tttggcgcaa aggagtgact tatgaaagcc
     7381 tggtgaaaga gatggatgaa gcgttgcaat tcccaggtgt gagtaatgcc tggaccatgc
     7441 ccattaaagc gcgtaatgac atgctcacca caggaattcg aacggctgta gggattaaaa
     7501 tttttggtcc tgacattaaa aagattgaag ccattggcaa agaaattgaa atggaggcca
     7561 aagaagtgaa gggcaccagt acggtttatg cagagcgggt ggctggaggc tacttccttg
     7621 attttcaaat taatcgggac cagttggctc gctatggttt aaccattatg gatgttaatc
     7681 gcatcattga atccgctgtg ggtggggaaa acattgccac cacgattgag gggcgtgagc
     7741 gttacccagt gaatgtgcgt tatttgcgag aattacgcga tgaccccgac aaactcaatc
     7801 gtattttagt caaaacaccc agtggtgcgg aggtacccgt tgcgcaattg gtgacgctaa
     7861 cttttcgttc aggacctgcc atggttcgcg atgagaatgg catgttggca ggttacgtct
     7921 tcattgacat cagtggccgg gacattggcg gctatgtgga ggaattaaaa caagtggtca
     7981 acgccaaagt gaaattgcct cctggctata cgcttgcttg gtcaggtcag tacgagttca
     8041 tgcaacgggt ctatgagcga cttaaaattt ttgtgccact gactttggcc atcatttttg
     8101 tcttatttta ctttaccttc cgcaccatca ccgagacctt gatggtgatg cttggggttc
     8161 cctttgcttt atttggtggc tttttattgc tttatttatt gggttacaac atgagtattg
     8221 ccgtttgggt agggatgata gcgcttgctg gtgttgcggc tgaaaccagt gccgtgatgc
     8281 tcgcttacct tgattctgat tacaacgaac aaaaagaaaa agggctgctc aaaaccttgc
     8341 ccgatttgat tcagctggta caactttgtg cggtctcccg cattcgtccc atggccatgg
     8401 cgggtcttgc taacatcatt ggcctattac cggtgatgtg ggctacaggc attggggctg
     8461 atgtgatgaa gcgtcttgca gcacccatgg tcggtggggt tttttctgcc cttttactga
     8521 ccttaattgt gattcctgtg gtttatgtga tgtggcgttg gcaagtggat ttgaaaaaaa
     8581 cagcagactt aacggagaca agatgaaatt aaccttttta ggagcaactg agacggttac
     8641 cggctcaaaa tatcttttga ccattggttc taaaaaaatc ctgattgatt gtggtctttt
     8701 tcaaggctac aaagaattac gattacgaaa ttgggctccc ttacctattg acccccatga
     8761 catcgatgcg gtgattatca ctcatgcgca tattgaccat acgggttatc ttcctttgct
     8821 cgtcaaaaat ggattccaag gcaaaattta tgcaacaccg ggtaccaaag cactgtgttc
     8881 cattttgtta cccgacagtg gacatttgca ggaagaagaa gcacgcttgg ccaataagta
     8941 tggattctcc aaacatcaac ctgctcttcc tctttatact gaaaaagaag cacgggcggc
     9001 attgcagtat tttgaaacga ttgattttga taccccgcat caattgttcc atgattttag
     9061 ttttgagtat catcgtgccg gtcacatcgt tggtgcggcc atggtcaaaa taaaaaccca
     9121 aaaaggttcc attgtgttta ctggagacat tggccgtcct tatgatcccg ttatgaaagc
     9181 ccctgccttc attcaagaaa cagattatct ggtgatggag tccacctatg gtgaccgatt
     9241 gcatgatgcc acagatcctt tgcctcaaat ggctcaggtg attaatcaaa ccgttaaacg
     9301 agggggctca gtcgttattc ctgcgtttgc agtagggcgt gcgcaaagtc ttctttattt
     9361 tatttatgaa ctgaaacgac aagggcaaat tcccaaagac cttcctgtct ttttagacag
     9421 ccccatggcc attgatgcca ctcatttact ctgcacttat aaagaagacc accatttaac
     9481 tgcagaacaa tgtcggggtt tatgccgtgt tgccacctat gtgaacacac ctgaggagtc
     9541 caaggagatt gaccgtcatc acatgccgca aatcatcata gcggccagtg gcatggcgca
     9601 aggaggccgc attctgcatc atttaaaagc ctttgcacct aatcctaaaa ataccctact
     9661 ttttactggc tttcaggcgg gtgggactcg tggtgctcga attgtgaatg gggagaagca
     9721 agtcaaaatt cacgggagtt ttattccgat acaagctaag gtcgtagtca tgagcagtac
     9781 atcggctcat gccgactacc tggaattgtt agaatggcta actcatttga ctaatccgcc
     9841 taaaaaggtt tttatcaccc atggggagcc tcacagcgcc caatcgctaa aacagaagat
     9901 tgagaagcaa ttaggttttt cttgcaccat tccttcttat ctgcaaacgg aggaattgtg
     9961 gtgagtaagc aaacctctca tgggcttcgg ttaaaacatt tagggattaa aacctatcac
    10021 gaagctatta tttacatgcg cgaggattgt catgtttgtc attcggaagg attcgaagta
    10081 cagacccgta tccaagtgac tttaggtcgg cgctctatta tagccactct taatgtggtg
    10141 acctcagagc tacttcagcc tggtgaggct ggtttgtcag attatgcctg ggattcttta
    10201 tacgccaaag aaggcgatga aattcaggtc tcccatccaa aacccttgga atccttaagt
    10261 tatgtgcata ccaagattta tggcaatgag ctgtcttttg agcaaatgaa ggtcatcatt
    10321 gatgatgtgt taagtggtcg gctttctgat gtacaaatct ccgcttttct agcggcaagt
    10381 agcgcagggc gtctaacccg tacggaaatc atgaaactca ccaaagccat gatagacagt
    10441 ggtgatcgct tatcctggtc ttcacctctg gtcgtcgata aacattgcgt gggaggattg
    10501 ccgggaaatc gcacgactct gattgtagtt cccattgtgg ccgcttttgg attgatgatt
    10561 ccaaaaacat catcgcgtgc cattacatca cccgcgggga ctgcggatac catggagact
    10621 ttagctccag tccacttaag tccccaaaag atgcgccaag tggttgaaca agaaaacggc
    10681 tgtatcgtat ggggtggggc tgtgagttta agtcctgctg atgacgtgct catccgtgtg
    10741 gaacgagcca ttgatttaga tagtgaaggg caattagtgg cttctattct ttctaaaaaa
    10801 attgcaacag gtgccacaca tgcggttatt gatatcccag taggacctac tgccaaagtt
    10861 aggaatcagt ctatggcttt ccttcttaaa caattgcttg aggaggtagg taacgagtta
    10921 gggttggttg tccatacgat attgaccgat gggtcacaac cggtaggaca tggcattggc
    10981 ccatcgttag aagctcgcga tgtaatggca gtattacaag ggttgcctga tgcccccaat
    11041 gatttgcgtg aacgggcgct gacgcttgcg ggtgctgcgt tagagtattc ttcgaaagtt
    11101 caaccgggtt taggcaaatc cattgcgaag caattattgg agagtggaca agcgtttaag
    11161 aaatttcagg ccatttgtga ggcccagggt gggatgaggg aattgaccaa ggcacgtttt
    11221 actcatcctg ttgtggctgc aaaggcagga aaagtctccc tcattgacaa tcgaaagctt
    11281 gcaaaaatag ctaagcttgc tggtgcgcca aaatcgaaat cggcgggtat tgacctccat
    11341 gttcatttga ataaatccgt tgaaaaaggc gagcctttgt ttacaattca ttcggagtct
    11401 tcaggggagc tcaattacgc ctgtgattta cttcgcgaca aacaagatat cattatttta
    11461 ggagaaaact cttgaagcct ctgctatttt cattatttga ttcttcagaa attgccagac
    11521 aattacaaga gatacttcaa gtggaggcag gtaaagttac tttccatcgt ttccctgata
    11581 ctgagtggta cttaaaaatt gattcagagg ttaacaatcg cagcattatt gtagtggaca
    11641 gtttaaacca gccggataaa aaaatactgc ctttgttgtt ttttgccaaa acggctaaag
    11701 aattgggggc tcaaaaaatc ggtttgattg ctccttatct ttcccaccta cggcaagata
    11761 aacgttttca cgaaggagaa ggaattactt cacgttattt tgcggacatt ttatccactt
    11821 cactcgattg gttgatgacg attgacccgc acttgcatcg ttatcactcg ttagatgaaa
    11881 tctactcgat tcccactttt gttttacatg ccacatcgat gattgccaca tggattaaac
    11941 atcatgttcc taagccaatt cttattggtc ctgatatgga aagtgaacag tgggttgccg
    12001 acattgcaga aaaaagctct tttccttatg caattttgga aaagattcgg cgtggtgata
    12061 aagaggtggc tatttcaatg ccattcatcc ctgaacttga atcctcaact cctgttttag
    12121 tggatgacat catttccact gccagaacca tgatagaaac ggtaaaacaa ttgcaacaaa
    12181 ctggggttaa gtcagtcctt tgtattgggg tgcatgcact ttttgcaaaa gaggcttatt
    12241 ccctattatc ggggataaaa ggcgtgcaaa ccatcacatg taacaccatc cgtcatgcaa
    12301 ccaatacggt ggatgtgagt gatttgattg tggaggcttt ggtaaaaaac aagtttgcca
    12361 aggccataaa aaagtaagat gaaaaagtgt gttttattta caaggagcgt cacatgtcgt
    12421 tttttaaaac agtcagggtc attgggattt tcattggact ggtttcggca accttttttt
    12481 tgaactcgtg taatggcgcg gtgaaacagg gttcacaaga ggttgaaggg ggctacggag
    12541 gacacagcgc gggtgggcat ggtggtcatg gcggcgctgg gcattaagta tgcccgctgt
    12601 cttggtaaat caagttaaag cgtactcata taacagatag ggcaaaccat gagacacgac
    12661 catcaccaag gacataccca tactgacaaa gagcctactt gctgtcacaa agagcatggc
    12721 ctccacaagc ggcaagacac ggcttctaaa accgatggta ccgtcatcta tacgtgtccc
    12781 atgcacccag aagttcgcca atcaaatcct ggcatttgcc ccatttgcgg tatgacttta
    12841 gaaccggaat cgataactac tgaggaggga gcgagtcctg agtatctcga catgagatgg
    12901 cgattttggg tggggttaat tcttacttta cctattgtag tgctggaaat gggaacccat
    12961 ggatttcagc atttcatctc tgctaatttt tcaaactgga ttcaactgct attagctaca
    13021 cccgtggtat tgtggggtgg ttggcctttt tttgtgcgtg gttggcactc tctaaaaacg
    13081 cgccaattaa acatgtttac tctaatcgcc atgggcattg gggtggcttg ggcttatagt
    13141 atggtggctg ttctgttccc tgggatcttt ccccttgcct ttcgcagtca attcggcaca
    13201 gtggctgttt attttgaagc ggcggcagtg attacaactc tggtcttact agggcaagtg
    13261 ctggaattaa aagcacgaga acaaacgggc agtgccattc gtgccttgct gaaattagct
    13321 cctgaaagtg cccatcgcat taaagaggat ggtggtgaag aagaagtatc gcttgataaa
    13381 gtgcacgttg gggataagct tcgtgttcgt cctggtgaaa aaatacccgt ggatggtgaa
    13441 gttctggaag gacgaagttt tatcgatgaa tccatggtga cgggcgagcc tattcctgtt
    13501 acaaaagaag cgggcgccaa agtgattggt gcaacaatca atcagacagg cagttttatc
    13561 atgaaagcca tgcatgtggg cagtgatacc atgctggcac gcattgtcca aatggtcagt
    13621 gaggcgcaac gaagccgtgc gccgattcag cgattggcgg acactgtctc aggttggttt
    13681 gtgccggcgg tgattttggt tgcggtgttg tctttcattc tgtgggcact gttcggccca
    13741 caaccagcct ttagttatgg attaatcgct gctgtttccg tactcatcat tgcttgtcct
    13801 tgcgcgttag gcttagcaac acccatgtcc atcatggtcg gagtgggaaa aggcgctcag
    13861 agtggcgttt tgattaaaaa tgctgaaagc ttggagcgca tggaaaaggt caatacgtta
    13921 gtagttgata aaacaggtac cctaacggag gggcgtccta aactgacgcg tattgtcact
    13981 gatgatgagt ttgatgaaga ggccattttg acgtttgcgg cgagcatcga acatcaaagt
    14041 gagcatccgc tcgccactgc cattgtcatg gcagcaaaag aaaaacaatt gtcattgggg
    14101 agggttgaag actttgaagc acctactgga aagggagtga taggtaaagt aaatggctat
    14161 cgtattgccg ttggtaatgc acgattaatg caagagtatg gcagtgataa gcttgccctt
    14221 tttgaaaaag ccgatgaact tcgcagcaaa ggcgcatcag tcatgttcat ggccgtggat
    14281 ggtaaaacgg ttgctctttt ggtggtggaa gaccccatta aatcgactac tcctgaaaca
    14341 atacgtgagt tgcagcaaag cggcatcgaa atggttatgc tgaccggtga tagtaaaaaa
    14401 acagcagaag cggtagctga tacccttggc atcaaaaaag tggtggccga aattatgcct
    14461 caagataaga gtcgcatcgt gagtgaatta agagacaaag gcttagtggt tgctatggcg
    14521 ggtgatgggg tcaatgatgc tccggccttg gcaaaggcgg acataggtat tgccatggga
    14581 acgggtactg atgtggctat cgaaagcgct ggtattacct tgttacatgg ggatttgcgt
    14641 ggtatcgcca aagcgcgtcg tttgtcagaa ataaccatga gcaacattcg tcaaaatctt
    14701 ttctttgcat ttatttataa tgtactgggt gtacccttag cagcaggtgt tttgtatcca
    14761 atcacagggt tacttttaag ccctatcatt gcagcagcag cgatggcact gtcttctgtt
    14821 tcggtcattg ttaatgcgct gagactacga cgggtaaggc tatgaacaaa agatgaaaaa
    14881 aacggttgtt aatagaaaaa aaacaaaaga gatctatact aataggaagc agtcggttgt
    14941 tcataaggca aaatcatggt gttcccagtt attatgattg tggttggtat tgtctttctc
    15001 atcggtggtg cctatttaat taaaagcgta ctgcaacaaa cgatccatta tcgttggcag
    15061 tggctcgtat atcttgtttt tattggtgcg tttattctaa gttattcggc cagtattttt
    15121 tatggtctct tggttgatcc ccattgtctt aattattttt atggcagtgt gtttttatta
    15181 ggcgctacct atgtttattt gtcttgtcgt cttatgacga agaccattca aaagatggaa
    15241 gtgatggatg atttaaaaaa tcgctatgaa ctattaaggc atcatgtcaa atacgattcg
    15301 ctgactggtt gccataacag agagactctt tttgaaatgt tgaacacacg gtttcagcaa
    15361 attacgagca atgatggatg ggctattgtc ctttttattg atatggataa atttaaggcg
    15421 gttaacgatc gttatggcca tgatgtggga gataacacgt tggcagcatt tggacaactc
    15481 ctaaggcagc gattgcgaaa ggatgatatc gtggctcgtt atggtggtga tgagttcatt
    15541 gctgtgatgg aacacatctc cttggatgaa gcgaaaaaga ttgcagaaaa tcttatcaca
    15601 gtagccaaac aatccttcaa aaaaggtatt ttatcgaagc ttaatctagg ctgctccatt
    15661 ggcatcactc agatgaatcc tcactcggct tcagtgaata gcgtgattaa agaagcggat
    15721 ttggcgtgtt atgcggcaaa gcaaagaaaa atcaatggtt ccgtttgtgt ctataatccg
    15781 atgattgcaa caaggaacga tacgattgat ttgggtattc atcacgttgc cgaataaaaa
    15841 atgaaaacac acaaaacagt cgtcttactg cagcagtgca ttaagcacgc taatagcgtg
    15901 cttttctgtt tttgtgttct acctcgccaa ttaatatacc actcttgttt aatggcattg
    15961 ggtgggagat actagagaat aaaataataa aaagaaggga ttaattcatg attcattttc
    16021 agatgaaacg caccatccta ttgatggttt ctgcactcct tgtgggctgt tcttataatg
    16081 ccaagttaac tcctgaggga gaaaaagtga cccttctcca atcaggtaag ccttccaaat
    16141 cctgtgtttt tttgggtgaa gtcagagcct ccgatcgcaa tggcatgagc caatcctatc
    16201 aatcccatga gcatttgatt caggatgaat taaatatcct aagaaatgaa gcagctctat
    16261 taggcgctaa cacggtttta gtgactcaac atcagcaaac ttatgaaggc aatcccaaaa
    16321 atgatttcgt agcagaccat tggatggtag gtcgagccta ccgatgtaag acgaattaaa
    16381 agttattttg agtcttatta tttatggaaa ataattctat gaggtggttt tcttgttggg
    16441 tggcgcttct attactgtat tcctctttgg ttacagcagg caacgtattg acccttcaag
    16501 aaataactca aattgctcta gcaaataaca aggatttaaa agcagctcgt tatactgttt
    16561 ctatcgccaa ggcgcgttta atacaagcag gtctttggcc taatccaagt cttaatcttt
    16621 ccaataacga cgatcgctta tttaatgacg aaggggaata ttcacgcagt gctggtttta
    16681 gccaagcatt tcctatttcg ggacgtattg caaaacaaaa gaccgtggcg cacttggatg
    16741 tgttaaaagc catggcagaa attagggaag cggagcgtca attaagcagc aaagtggcca
    16801 atgcgtatta tgccgctgtg attaccgagc gtcgcatgca acaggtgaat tacttattac
    16861 ggataaaccg cgagcttgtc gatgttatcc acaaccgtta tcatgcggct gaaatttcaa
    16921 agctggatga gaatgcagca cgcattgagt atctgaggat tggacaagaa aaacatcttt
    16981 tgcatagttt tagtatcagt caatatgccc tactgaatca attgatgggg cgggaagcca
    17041 attatccttt atctcttaag agggatgtca tcgcaggtaa aaaacttcct gagttagcct
    17101 tcttaaaaac attagccctt aaaaatcgtc ctgatcgtca ggccatcgcc ttatcgattc
    17161 atcgtgccaa cgccgacagg cgtttggcaa aagccgagcg ttttgcggat tggacggtag
    17221 ggcttggtgt ccaacaggat aaaattgtgg tggaagaggg accaccccaa cccgccgatc
    17281 gcactttagg ggtcacgctt tctgtgcctc ttcctctttt aaatcgtaat caggggcgaa
    17341 ttttagaagc cagtcacaca ggaacccaag cggtgatggc attacgtgcc ttggatttag
    17401 ttattgaaac cgaaatagca agtaattatg ctcaactgaa agcacttcaa atgagcctta
    17461 gggaaacgca aactgtttct cttaaattag cagttgagaa tgtcaaactg gcacgtgatt
    17521 cctatgagag cggacaaatt tcttttttga atgtattgca agtacaaaaa caacaaaatg
    17581 atttacaaac aacttatctc aatactttag aaaagtattt gcaagtgtat gtggctttat
    17641 gtacggccat tggtcctggt aaaaccaatg ggttttgccc ttatttagcg tatcaaagga
    17701 atggggatag taaatgagag gtggcagcaa atgagaagga tggcattaat tagtctgttt
    17761 cttagtctct tctttcctat tcaagggtgt tttgcccatg gagaagaaat tgaagtgagt
    17821 gaagggggaa ccaaaggccc agttcattta acacccgcac aaactaaaat gcttgacatc
    17881 aaggtagcgg aagcaaccaa tcgccccatg gctcaattac tgggtttaaa tggccaaatt
    17941 caattgttac ctgatgccca agccgatgtc agcattcgga ttagtgggag tgttacagcc
    18001 attgatgtga atttagggga tagcgtcaag gcagggcaac gtttggccac agtacagtca
    18061 aggttagtag gtaatcctcc tccaagtgtt gcggtcacag ctcccattgc aggcatcatt
    18121 gatgccagga atatcaattt gggccaagcg gttgagccca atactgttct ttttcacatc
    18181 agcaatcgcg ataaattatt ggtcgtagcc caagtgtatg aagaagattt aggcaaagtt
    18241 aaggtagggc aagaagtgac tattcatgcc ttaagttacc ccaagcggac atttccaggt
    18301 caagtgactt taattgaacc caatcttgac cctttaactc gtacagccaa tgtgcgaatt
    18361 actttggaca ataaggaggg actcctcaag actgggatgt ttgtgcgggc taatgtgatt
    18421 ttagcgcgta atgaggcagc acttgccata cctaacgctg ctcttttaca ggcggataat
    18481 gagcaatttg tttttgtgca aaatggggat acttacgagc gagtggatgt tcaagtcggt
    18541 gctgtggatg atgacttctc agaaattaca gatggcttgg ttcctggcga cctcgttgtc
    18601 acgcaaggca atcgtgagct ttatacatta tggcttagtg gtggtagtaa atttcaatcc
    18661 ggcagtcatg aggagcccca ataaatgttt actcgcctca ttgcctggtc tttacataat
    18721 cgccccttaa ttttggcgtt aacgattatt ctttgtttat tgggtggtta taccttaaaa
    18781 cgaatgccag tcgatgtttt tcctgaattt gcaccaccgc aagtggtggt acaaacccaa
    18841 gcgcctggca tggcaaccca agatgtggaa actctggtga cttacccctt ggaaagtgcc
    18901 atcaatggaa caccaggggt agctagtgtt cgctcaaaaa cctccgttgg attatcaacg
    18961 attactgtgg tatttgatga taaaacggac atctaccgca atcgccagct ggttaatgaa
    19021 cgtattcaac aagtagtaaa ccgccttcct ccgggtgtct cgtcaccggt gatgctgcca
    19081 gtcacctcag cggtcgggtg gttgcttaaa tatgctttgg tgagtcgcac ggaaagccca
    19141 gaaacactgc gaaccatttc cgactggact attcgtccgc gcctcttagc tttaggcggg
    19201 gtggcttctg tagtttcctt aggcggagaa gtcaagcaat atcaagtgca gcttattcct
    19261 tcgcgcatgc tggcctatgg catcacggtt gaagaagtgc gccaagcgtt aacaacagga
    19321 aatcaaaatg tgcctggtgc ttttgtccat caagccggta ctgaattcgt agtgagcacc
    19381 attgggcgga ttaaaacgct taaagatatt aaaaaaacac tgattgtagt ccgtaatggg
    19441 gttcctatta ccattgataa cgtggctgca gtcgctttcg gtggtgaaat taaacggggt
    19501 gatgcgtctt ataatgcaga acatgcggtg attggtactg tttctaaaat ttatggcgcc
    19561 gacacattaa ccacgacaca taaagtggaa caagctttag cggacatcaa aaaatcgttg
    19621 ccagcaggga ttgaattaat tacagaggtg tttcgtcaag ccaattttat tgaatcggct
    19681 attcataact taacgaaagc cctgattgaa ggagcaatta tcgtcattgc agtattgttt
    19741 ctttttttaa tgaattggcg agcttctttt attacgtttt tgtccatgcc ggtttctttt
    19801 attgtcggta tcctggtgct tcattatttt ggttttggta tcaattcgat gaccttgggt
    19861 gggatggcga ttgcgattgg ggaagtggtt gacgatggca tcatcaccgt tgaaaacgtg
    19921 gtccatcgct taaggcttaa tcgcatgagc gataaaccgt tacccgcgat tgaaattgtt
    19981 tttgatgcgg ttttggaaat tcgaagttcc gtggtttatg cgaccattat tattagcctc
    20041 gtgtttttgc ccattttctt tctatcagga atccctgagc ggatttttag tcctttagcc
    20101 attgcttata tcgcatcagt cttaggttct cttgttgtct ctattactat ggtgccggcc
    20161 ctttgttatc tccttttagt gcgaaaaagt gaaaaacagc aagataataa ggtggtgatg
    20221 catgctttat ctcaggagga acgttatcat ttggcggaag aagaagcagc caaacaatct
    20281 caagcggaaa ctcgttttgt gatttggcta aaaaaacatt ttttaatggg gttaaattgg
    20341 tcattggacc attgtaaaac ggtcgtgact atcgcaattg gcgcatttgt ttttgcttta
    20401 gccttacttc ctttttttgg gacttccttc ttacctgagt ttcatgaggg caattttatt
    20461 attgtgatga gtacgttgcc aggaacctcg ctggatgaat ccatgcgctt aggcaatcaa
    20521 gtcagtaaaa ccttgttgaa gtatccgcaa gtgatttcca ttgctcaacg ggcaggacgc
    20581 agtgaattgg acgaagatgc tctaccaccc aacatcagtg agttcgatgt gcgcctggat
    20641 tttggcaaag acaagagcat atctccggat gaattattgc aacgcattcg agcggatttg
    20701 gcaaacatcc caggagcgct gtttaatgtg ggtcaattta ttgcccatcg tatggatgaa
    20761 gtgctatcag gagtcagagc acaagtggcg gtgaagctct ttggtgataa tcttgcgaca
    20821 ttgaacgaac tgggtcaatc agtcgaagtc ctattaaaaa ccgtaccagg aatagtggat
    20881 gttaacaaag agcaacaaat taatgtaccc cagttggtca ttaccattga ccgagaaaaa
    20941 gccacgcgtt atggcattaa tgtggggcaa atttctgagg atgttcaagt tcttttgaat
    21001 ggcgtaaccg tgtcgagtat tctcgaaggg cagcgaacgt ttgatttgta tttgcggatg
    21061 agtgaaacag ggcgagatag tgttaaagcc attcaaaaca tgctgattga cgctcatgga
    21121 acagcggatc agaaaactaa aattcctttg cgtgcggtag ctgaaattcg cctagaaccc
    21181 cagccttttg caattaatcg ggaaaatgtg caacgtgttt tggtgattgc ttttaatgtg
    21241 caagagaggg atttaggtag tgtgattcaa gaagtgcaac aacgggttaa ggaaaaggtt
    21301 acattgccca gcggttattt cattcagtat ggcggtcagt ttgaaagtca gcaacaagcc
    21361 tctcgcgtga ttattctttt tggtgcactt gctattttta tcatgttaat cctgcttcat
    21421 aaggctttcg gaacatttcg tgaggcactg cttgtgatgt tcaatttgcc tttggctttg
    21481 attggtgggg tcgtatcatt gtttcttgcc ggtggtaaca tgagcgtggc ggctatgatt
    21541 ggttttatca ccttatttgg aattgcggcg cgtaatggga ttattttagt gagccactac
    21601 aatcaattgc gtgtccaggg acaaccacgt gagcaagtgg tcattcaagg aaccctcgat
    21661 cgcttagtgc ccgtactaat gaccgccgca acagcagcgc ttggcttaat tcctctgtta
    21721 tggggttcgc ctgcaggaaa ggaacttgag cgtcctttag ctcaggtgct tttgggagga
    21781 ttatttactt ctactgtact caatatgttt gtagtaccta ccgtgtataa tgctattgaa
    21841 gtatggcgag aacttcaata aaacagaaca aggagaaaca agatgaagca cgttactttg
    21901 aaacaaaaaa gccatttttg gttagggctt atcaccagtg ttttgttggt ttgtgggtat
    21961 tctcaagcaa tggctgattc tactgaagag tcaacacaat ccattcccaa aacgattcca
    22021 gccatttggg aggctgtaga taagcatgcg gcatccatta atcaagtgct tagtagtgat
    22081 gatttaacga caatacacca gcatgcattt gccattcgtg atttagtcaa cgcattacct
    22141 gcattaagca aagatttgtc agaggagcag aaaaaaacac tgcagcaaaa tctaagctat
    22201 gtcagtcaat tggccactcg tcttgataaa tcaggcgatg ccaatgataa aaaagggaca
    22261 caagtcaact gggaaaaatt gcaaaaagtg ttggcgcagc tgcgttcttt gtatccttca
    22321 ggtgtcacta attaatcaag gaatgagtca tggtgtattg tgttgttcgt gcgtggtttg
    22381 ttatagactg ctgtcgtttt aaggaacgag gctgcagacg caaatggcca tatcagaggc
    22441 acagacatca tgacttacaa agcgagattt ttatgcatta atttggtttt aaatccaaag
    22501 taatgacatc gtctgctttc ccacttttgg ggatgatagt cactgtgcct tttgtaaaag
    22561 agccaacctg gtagctttct tcttctaggg tcacggggtt tcctttttga tcccgtgcgg
    22621 aggataaatc cgcaacttgt gctaactctt caagactaat cgaggagccg cgatacaggt
    22681 gtttcgtttg gtttgtagtg tcagtggctt cagtggttcc atgacccaag gtcacaaagg
    22741 atatggcgag catggttaat aaatacaata gaaaattgat ttttttcatg agattcatcc
    22801 taatcaaatg aggtattacc ataatagatt atctacggcg caacaacaaa cggttacgac
    22861 tggattattt gttatttaat ctcccttaat cacttccttg agcagtcgtt gactttaata
    22921 ggtcttgtcc atatcagtca tccaatggag agaaaatttt tgtaaaaatc ttatccctag
    22981 tccacaatag ataaagaggt gggttattca ggatggtggt gatgcatggg caaggaaaat
    23041 accgattcgc cttatcaaat ggggcatgat tttttttctt atcaactgga ttgttggcag
    23101 cgttctattt tgttttggaa tactttgcgt gaacgcgcca ataatatgat ggagcacgaa
    23161 caacaagggt tgccaccact gcttaatttc aaatatgaat ttgttttgga tgggaaatca
    23221 ttggagccta aaacgaatta tgcattggtt aaaattcttg aagtcggtga tgtgtgcttt
    23281 gaagaatgtt ttgatcctca tgctcatcca gttattattg tggatcccag atcaggccat
    23341 gggccaggga ttggtggttt caaacgggac tctgaagtag gcatcgcgtt acatagtggt
    23401 catcctgtgt attttgttat tttttatcct aatccggtgt ctcatcaaac gctcgccgat
    23461 gtcctgatga ctttgcgcca ttttgttgaa aaagtccaag cctggcatga aggcaaagcc
    23521 cccattcttt atggcaattg tcaagccggt tggatgctgg ctttattggt ctctgattgc
    23581 gtgggatcgg ttggcttgac ggtcatgaat ggctcaccag tttcttattg gtccaacagc
    23641 gaagaagaag ccaatcccat gcagttattg ggtggactct tgggtggttc ctggagtgcc
    23701 cgcttcctat cggatttaaa agaaggaatc tttgatgggg cctggttggt ctctaatttt
    23761 gagttactca atcccaccac ggccatttgg gataagtatt atggcttatt tgatgaaatt
    23821 gatggggagc gtgagcgatt tttggagttt gaacgttggt ggaatggttt ttatcaattc
    23881 agtcaagaag agataatggc cacagtgaat aatcttttta ttggcaatca actagagcgt
    23941 ggtgaaatgc ggattcataa aggatgtgtt tacgatttga aacgcattca aagccctctt
    24001 gttctctttg cttcccaagg cgatcaaatt accccaccga ggcaggcttt acattggata
    24061 agaaccattt atcctacgac acaagccttg aagaaggcca aacaacgagt ggtttatctg
    24121 cttcatcctt ccgttgggca tttaggaatc tttgtgtcgg ctaaggtcgt tcgattgcac
    24181 catcgtgcta ttttagagca tagtgatgcc attgagcaat tgcagccagg gctttatgaa
    24241 atgataatcg ttaacccaac gggcgatccc gattgcagta aagaacaata tcatgtgcgt
    24301 tttgaagcgc gagaacttac agaactttgc acaacgagct ccacgcaacc gtttgacaag
    24361 gttcgtcaaa catccgaagc caatgactca atttatcaaa aagttatcca accttctgtg
    24421 cagagcttaa gtaacccttt tttatcctgg tggcttgaga aaacgcatcc catgcgccta
    24481 agtcgctatg tgttttctga aaaagtgaat ccactgatga aagcaatcga actattaagc
    24541 ccacccattc aagccaatcg ccgcatggct acacagagca attgctttaa aaaaatagag
    24601 catggtgttg cgcagatgat gagggattcg ttggaatcct cccgaatcat gcgtaatgag
    24661 gtgatgacca attggtttga agccctttat aaggatcccg attaacaggc gaggacgtta
    24721 attcatagag cgtatggcat caaactcctc gtcaaggtcc atttcatttt ctaagtccct
    24781 ggcttcatga acctctttct catcaaaaag agatttagtt tctctaattt tttcttcgtg
    24841 gtcattctct gcaaaacaat ccttagcgtt cataacaatt ccttcaagat tattgttctt
    24901 gttcttgcca acgactaaaa acaacccggt ttattgttgc tatttttatc tctggcattt
    24961 agcataacgg ataacaattc aattgacaaa aatactaaat aagaaataaa aagggggtaa
    25021 ctgaatagcc atttggctat ttttttataa gaagaaacga gggaatagga tgttggttcc
    25081 ccaaactgga ataagaaacg catcgaccat gatagaaatt atccctaatt ggcaccccat
    25141 ttttgtccat tttactgtgg ccttatttac tgtttcggtg attctttatg ccctaatcta
    25201 cctagcttct tacactcatt ggaacaccaa gcccttgatt gtcgaattgg aaatagtagc
    25261 tcgttggtgt ttatggctgg ccgcattaag cacggtgacc acggtgtccg ctgggtttta
    25321 tgctttttat acggtgaaac atggggctat ggtgcatgcc gtaaaagtga ttcaccgcaa
    25381 ctgggcactc ggtagcgcga gtgccatttt acttatggct ttctgggtgg tttggcgtta
    25441 tattaaacgc caaaaaccga cgctgatttt cttaatggta ttgttcctgg tgcaagtgct
    25501 gttgcttacg acggcttggt atggagcgga attagtctat cggtatgggt atggtgttct
    25561 acctgtcaaa gcaaaacaga cggtatcgcc gcattaatac aatttttatg gaaggataca
    25621 aacatggtac ataaggactt gcgttgttcg ataaggaact attgcttcat tttttccttt
    25681 ttactgctca gttcttctgc tttgtttgcc cagcatgagc aacatggtgc ttcaacacaa
    25741 ccgttacctt ctcccaagac tacaccgtct caatcgaaac ctgcacaata caaaatggaa
    25801 caacctatcg ctaaaaaaca atctgtcaga acagtgaaac cgctcactat gacaggtgga
    25861 gcaaagcgta cggttaattt ggtagtcgct tataaaacgg tgaattttgc aggcaaacta
    25921 agacgcgcta ttgccgtcaa tgggcaaatt ccagcaccga ctctgcattt taaagaaggg
    25981 gatgatgtca ccatcaatgt gtacaaccat ttggatgagg gaacgtccat tcattggcat
    26041 ggtcttttag tcccttggca aatggacggg gtggatgagg tgagtcaaaa accaattccg
    26101 cctggagggg tgttccatta tcgctttaaa ctgtaccaaa gggggaccta ttggtaccac
    26161 gcccatgcca aggttcaaga acaagaaggt ttgtatggga cttttattat tgatccaccc
    26221 aatcctccaa gctatcacta caccaaagat tatgtggtgg tgttatccga ttggagcaac
    26281 acccctgcgg aacaagtgct tgcgaattta aaaaaagacg gcgactatta cagtcctcga
    26341 tttcccctgc aaccctcact catgaaattt cttcatgatt atcgtaacgc ctctcctgaa
    26401 gagcgaaaaa aattaatcgc cgattataaa atgatgcaac aaatgcgtat gagcatctat
    26461 gacttaagtg atgtggcgta tgatgcgtat ttattaaatg gtcatcctaa atcgcatcct
    26521 tggactgctc ctgtgaaagt aggggatacg gtgcggctac gcttcattgg cgcaggtgca
    26581 agtaccattt atcgggtcaa aatccctgat gccaaagtgg acatggtgca cattcaggga
    26641 aatgatgtgg acccttatcc cattgaggat ttttggattg caccgggtga aacctatgat
    26701 atcctggtga ccattcaaaa aaacaagcct tacattattt atgccgagtc gattgatact
    26761 ctgggtaaag tctatggggc cttaatgacc catcctaatc aagtcgtcaa ctatcagcaa
    26821 gtgactcctt tccctgagcc acttcctgtc acaagagaaa tgatggccaa tatgatgcag
    26881 tctatgaatg gcggggcgat gagtggaaat caacaacacg ccttgatgaa caaaaaaaca
    26941 tcatcaccgg caatgaaacc ttccatgact atgccttcgc actccaagac aatgagttcg
    27001 caaagtgcta atcactccat gtcctccatg actgcccaaa agaaaatgga caatgcatcc
    27061 aatcattccg cacacaccgc atcaatgaag tccaaatcct catcgacctc gatgaacaag
    27121 agcatggaca tgccgggcat gaatcatggc gcaatgagtc agagtaagac gtctaactct
    27181 gctcaaatga aatctgactc ctcgtcgacc tcaatgaaca agagcatggc catgccaggt
    27241 atgaatcatg gggcaatgag tcagagtaag acgtctaact ctgctcaaat aaaatctgac
    27301 tcttcgtcga cctcaatgaa caagagcatg gccatgccgg acatgaatca tagcgcaatg
    27361 ggccagaata agacgtctaa ctctgctaat gacacgtcta tgaataggaa catgaagtct
    27421 gacaggccta tggagcaggg catgtcgatg aatggctcca tgaacatgaa gatgcccatt
    27481 gagccgacca tcataggcga taaaatagag ccacctgatt cggcaaaagc caccacctta
    27541 ggaaccaaat accaagagtt aaaaggggca gtgaaaacca ataatccgaa taaaccagtc
    27601 gatgggatta ttaaaatgga attgtttggc tacatggacc gttatatctg gtttattaat
    27661 ggtctaccgg aatataaggc caaaccgatt ttgattgagc caggaaagcg ttatcggatt
    27721 atatttacca ataattcgat gatgcgtcac cccatgcata ttcacggcca ttggtttatt
    27781 ttacgcaatg gtcatggggc ttacgatccg ttattgcata ccattgaagt ccctccagga
    27841 gcgactgccg ttgccgattt tgatactgaa gccagcggtc aatggttttt ccattgccac
    27901 catcttctgc acatgaccgc gggcatggcg cgcgtatttc aatacaccac catcattgag
    27961 attgccaacg gcactcgaaa gccagacaat tacgcctatc agcaagccta catcaatcgg
    28021 ccggtcgtac gagaagacga agtcatgcca ctggatgctt ctctcatcaa acaccctgca
    28081 ggacatcatc aaggcttcta tcgttcaagc tatatcgagc tgggcgaaga cccttttcat
    28141 aatgctcagg aaatgacgtt tagaggcctc tatggcccag attacaataa gctccagttg
    28201 tatactgagg acgctgaaat ttataaagga accgttgaaa acgcagacat cgatgtgttc
    28261 tattggcatt tgattagcca attttgggcg gttaaaggag gagtcaatta tttttatcgc
    28321 ccgggaggcc cttattggca acctggtatt ggtattgaag ggttaatgcc ttggtatatt
    28381 gataccaaca ttagaacgta ttatcgtgat ggcagtgtca agtttgacat tcagttagca
    28441 cgggctaacc agctgaccaa taacttcttc ttcctcacag gaattcgcag cattttggcc
    28501 actcataccg tcgtcaaaaa cgagattggc aatggcttaa accaaatgcg ttatatcctg
    28561 agaccttatt atcgtcttaa gccaggactt aatatttttt cagagtatga gcatgatgta
    28621 gagtatggtg ctcttaagag gattcttcga agtcagggag agcctaccac tcaagataca
    28681 atcacgttag gagttgctgt tcttttttaa aaaagccaaa tatacattgg aattatttta
    28741 tgaattcata ggaataaata gtcaccttca tgatattaat ccatccatga ggaatatatc
    28801 acctatcaat gaccttgttc attggggtgt tgagctttct cttcccaaga aaatccttcc
    28861 tcatgtcttt aggatgggga caatttatct gacaatactg ggttaaatta tcctcagttt
    28921 tgtaatttaa cccagggtcc tggatttttt aatgattgat ctgcaggtca tcattttcaa
    28981 ataatggagt ttgcttaatt tctaaatttc tttgttgtaa ttggtcaact ttcacatggg
    29041 gacagtaata ggcattagct attacggaat ggatattaat agttgttaag ttttttgatc
    29101 cccttacata tctcttatga gcttcacttg gtgtatgggt tacaagaacg ataacatttg
    29161 ctcctaactt cgcaccttca ttttttaaaa aattattatc atctttttct agatctttta
    29221 aagaggtgcg gatatccaca acttcatgta catttgggtt tttaataatc cctaagaaac
    29281 ggcaatttcc tgtaggatag gaaaaatata agttaacatt tttcgcatct gcatttactt
    29341 gacgtaatcc accacagcc
//
LOCUS       DACTJE010000022        28537 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.022, whole genome shotgun sequence.
ACCESSION   DACTJE010000022 DACTJE010000000
VERSION     DACTJE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 28537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28537
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.022"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1124)
                     /locus_tag="I9760_000736"
     CDS             complement(<1..1124)
                     /locus_tag="I9760_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAT5042523.1"
                     /translation="MTEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYEND
                     QDRYLLERSIDISSKEMRDINAKLEYAQDIAQIGYWHYDADTDTATWSKGLFKILGLP
                     PSNVALNYSSFLDLVHPNDKAGLKNVVEKALKEKEDYDYELRVRNGDNDYKWYRTIAR
                     ANPHKNEISGVLMDISKRKIIEEQVHELNNKLVSTARLAGMSEVATSILHNVGNILNS
                     VNVSAIILKKNISKIHYKKLSAIKTMLDENINQLSEYLLQNPKGKLILPYLDALSKTL
                     HEEYEENMKESNSLETNINHIKEIIAMQQSLSGLSNLTEKISIHDVILNAVKSVILDK
                     HQIDVRIEIEPDINEFITDKSKLFQILTNLLANAKDSVLL"
     gene            complement(1147..2310)
                     /locus_tag="I9760_000737"
     CDS             complement(1147..2310)
                     /locus_tag="I9760_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042524.1"
                     /translation="MKIESFQYIQNQGWSIPTFPELDSENTLILVFASPIFRANSKPI
                     EELAQAYKKSKIIGCSTAGEIFGPNIFDNSISVAVVQFESTKLQLAKTEVKSTEESYE
                     AGVHLSKSLHQKQNPQSIFVLSDGLNVNGSELIKGLNQHAGKGVIITGGLAVDGADFK
                     NTWIIYDGKIVDHCIGAVGFYGDQIHVGHGSRGGWDIFGPERVITRSKANILYELDNQ
                     PALKLYKTYLGDRASELPAAGLLYPLAIRNPSAPDSQHIVRTILGVNEEEQSLIFAGD
                     LPQGYYAQLMRANFDRLIVSANEAGENANDRLFKNGNLNTQPVLAIDISCVGRRLVLG
                     ELTEEETETTLQSLPPKSIQIGFYSYGELSPLATGTCELHNQTMTITTLLESP"
     gene            complement(2479..3360)
                     /locus_tag="I9760_000738"
     CDS             complement(2479..3360)
                     /locus_tag="I9760_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT5042525.1"
                     /translation="MDIRDIKSFLAVVEYHSLTLAAKKLHVTQSAMSKRIRKIEDELN
                     VRLIISEGSKLIFTETAKQLIPYARQMLSAHQNMVHTLKNSQQFTQSIIIGASVYVSH
                     YVLPGFFKYLKETHPTIQAHLKTISEKEVEYILNHGTVDLVICPAREVSEKLLGNCLL
                     WEEKLNLVVAANHELAHRTSELSLSELSEYPAILTEKGAALRDKLDTLFEKNQLVLIL
                     GFEMSTIDAIKSLVEYGLGWSYLPDSLLSEKLKILKIKDLELTISFHAFYLKKRISDR
                     LINDLLSNFKKWQNRDN"
     gene            complement(3582..4766)
                     /locus_tag="I9760_000739"
     CDS             complement(3582..4766)
                     /locus_tag="I9760_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT5042526.1"
                     /translation="MHLKRTLSLTLLAFYGLGTILGAGIYALVGEVAKEAAQFTPLSF
                     LIASIIALFTAISYAELSSRLPLSAGSALYVRRAFHQKWLSGLIGWIVVLTGVISAAT
                     LSHGFVKYFQLFLPLPPSLIITILTVILAGVAVWGIRESATLILYMTLMEVGGLLMII
                     YYGRYSFASIDIQSFSLPHSFDGVLIGAFIAFYAFIGFEDMVNTAEETINPEKTLPKA
                     IFLALISATILYVAVAWVIVSTIPSNALVKTDMPLIEIIKLQGQSPLLFSIIALISIT
                     NGILVQIIMASRLIYGMANQQNAPQIFSFVYSKTQTPVNSTLLVSLIILLFAYALPIT
                     TLAKLTSSIILCIFIIIHASLIKIKLTKKKPTNAISFPIVFPMISILLTLAFLIIQFF
                     LK"
     gene            complement(4834..5610)
                     /locus_tag="I9760_000740"
     CDS             complement(4834..5610)
                     /locus_tag="I9760_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAT5042527.1"
                     /translation="MSRREWPANDYAIGSYIQATVAEEYLPYLNMKPADKVLDIGCGN
                     GAFTKNILMKVPQGSVLGIDASENMLHLAQDVSKEYPNFSVQKADVLTMDFHLQFDYV
                     VSFWCLQWACANIQKAFLNIVNALKPGGKFLTLFPAGDDPFIMSYYALKKSGQFASLH
                     DFIPPVDYSHLKNLEKKLESLSCQELKVKLCRQSITLPSLDVFRKFVNGIAFYQGQLP
                     NEEIQKINEAMVQYYDNECQAKYNGEYQFNFTIYLVTGEK"
     gene            5818..7029
                     /locus_tag="I9760_000741"
     CDS             5818..7029
                     /locus_tag="I9760_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HAT5042528.1"
                     /translation="MFSQSQQKIVCVLYDDPKGGFPPNYARESIPELKQYPDGQSLPN
                     PDSIDFIPGEMLGSVSGELGLRQFLESNGHQLVVTSDKDGSDSVFARELKEATVVISQ
                     PFWPAYLTRDRIESAPKLKLAITAGIGSDHVDLQAAMEHNITVCEVTYCNSISVAEHT
                     VMMILALVRDFIPQYNTVIDGGWNIADCVSRSYDLEGMQVGCVAAGRIGLAVLRRLKP
                     FAVKLHYTDRHRLPVQLEQELNLTYHPSVESMVKVCDVVSIHCPLHPETEYLFDERLI
                     KQMKRGSYLINTARGRICDQHAVADALESGHLAGYAGDVWFPQPPAKNHPWRSMPNHA
                     MTPHTSGTTLSAQARYAAGVREILECWLGNKPIREEYLIVSQGHLAGVGSHSYSAGNT
                     TSGTEQAAELV"
     gene            complement(7135..8259)
                     /gene="ceg10"
                     /locus_tag="I9760_000742"
     CDS             complement(7135..8259)
                     /gene="ceg10"
                     /locus_tag="I9760_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg10"
                     /protein_id="HAT5042529.1"
                     /translation="MFTKSCAKATDQNRQELFMDSLLGDFQKMKEKALNQQQERRSKK
                     RELHCKSSEKTKKPNQYAALTHSQVQEVKAKVRTVNDKFHLNAEEKKLWELILLGNQL
                     AQNISSCDLPTDNEDDASLVKLTQIFADETLERTDLTWLNKILKIALYSRGSGFGNCQ
                     EKAFFVFALLLHQAQKPESLIHSLRLATFNNHFILIVNEQFLMDPWLNLAFPLSKGNQ
                     QLEIGYVFERFGRLVNYFSINQEGQCFTHTVREGGTIERDPSSEKDMANCIHSLLDHR
                     DYFDLSIVSEREIHTNTKRRKYENQEQEVPQPRQETSNQPTETVYTDSSHNTWDNEGA
                     ADDFLEHFEQTHPVYNPGFFSFGQDDVGLKPDLPKNLFKF"
     gene            8496..9179
                     /gene="lem2"
                     /locus_tag="I9760_000743"
     CDS             8496..9179
                     /gene="lem2"
                     /locus_tag="I9760_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem2"
                     /protein_id="HAT5042530.1"
                     /translation="MVIIGILMSKERIKKTALEIVKYIHGNIKACNIPNQKKENKFFA
                     PLVYLCQTECDKILSNTKISLLERLLKCAKIIGDLQSGNCMQQTFLAFQRLLMRLIED
                     KLSNFSTCIPISVMTISNHAFLIIDNDIVCDPWLNFVGDLKDYCFANMKRKEYFGIRS
                     DWTCFTNSEVYDEDSTEFTIQFVNALGVGISGFSEIKLANQLMQSSSMASDLGEDPVI
                     SIPKEFHRI"
     gene            9287..10060
                     /locus_tag="I9760_000744"
     CDS             9287..10060
                     /locus_tag="I9760_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAT5042531.1"
                     /translation="MNFLNNKIVMITGASSGIGQACARLFAAQGARLILAARRVERLH
                     ELSSGLKHHNNQEHYILPLDVCKKDLVRQQIESLPGQWNSIDVLINNAGLALDTLPLH
                     QGILEHWDTMIDTNIKGLLYISRLILPGMLERNSGHVINIGSIAGHECYPNGNVYSAT
                     KHAVHAISKSMRLDMLGSAVRVTEIAPGAVETEFSEVRWKDKQRAIDFYKDFQPLMAE
                     DVADAALYCATRPLHVDIEQMIIMPTVQASANHLSRKGR"
     gene            10579..10809
                     /locus_tag="I9760_000745"
     CDS             10579..10809
                     /locus_tag="I9760_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042532.1"
                     /translation="MNKLIVAVTAGASLLLLTGCAVDGVAIDDVSYESGYSNDYVYSV
                     GYYSNRPYWGNNYYSSPSFVGPYGYWGGDYDF"
     gene            complement(10862..11431)
                     /gene="efp"
                     /locus_tag="I9760_000746"
     CDS             complement(10862..11431)
                     /gene="efp"
                     /locus_tag="I9760_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAT5042533.1"
                     /translation="MAVYSTNEFKNGLKVMVDDAPCSILDCEFVKPGKGQAFTRIKIR
                     NLKTGRVVERTFKSGDTLPSADVADVEMQYLYNDGEHWHFMVPDTFEQYAVTENVLAD
                     AAQWLKEQDVCVVTLWNNEPIQVTPPNFVILAITETDPGLKGDTSGGGGKPATLETGA
                     VVRVPLFVQTGELIKVDTRKGEYVSRAKE"
     gene            11503..12483
                     /gene="epmB"
                     /locus_tag="I9760_000747"
     CDS             11503..12483
                     /gene="epmB"
                     /locus_tag="I9760_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAT5042534.1"
                     /translation="MRDTSLSWQKILAQGFTSTTDLLDFLELPRSEGNLFAEKQFPSR
                     IPLGFAKRMQKGNPKDPLLLQVLAKEDELSEADDYVIDPLSESNTLIKGLLHKYHGRV
                     LLTLTGVCAVNCRYCFRRHFPYQANNPGRRGWKEVCAYIANDSSITEVILSGGDPLLA
                     ANLVLEELLQSLEEISHIHTLRIHTRIPIVLPERIDKGLLDLLTNTRFKKVIVVHCNH
                     PQELDESVLRACSDLKKAACYLLNQSVLLAGINDDAGILSKLSHALFDYGIMPYYLHL
                     LDKVKGSAHFDMPLLRAQSIYHQLQSLVPGYLLPRLAREEPGRSSKTLLI"
     gene            complement(12552..14636)
                     /gene="ppk1"
                     /locus_tag="I9760_000748"
     CDS             complement(12552..14636)
                     /gene="ppk1"
                     /locus_tag="I9760_000748"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAT5042535.1"
                     /translation="MEFLMDTTLDNPDYYINREFSAIAFNQRVLMLANDERVPLLERM
                     RFLSICSSNLDEFFEIRVAGLKEKIAMSSGKLTIDGLRADEAFSQISHKAHALIDQLY
                     NIFNKQLLPALRKENIHFLETDQWTDDIHLWAKHYFKHEILPVISPIALDLAHPLPRL
                     INKSLNFIISLSGKDAFDRNINYAVVHAPRSIPRMIHLPSELCGDAHYFVYLSSIIKT
                     HINSLFPGMEISGCYSFRLTRNSDLFLREEEIDDLAKAVQREIFSRHYGHVVRLEIEN
                     KCPEKIVDFLLQKYHLRHEDTYYCDGPVNLQRYMSAINSIDRPDLNYPAFTPQYPKFS
                     KSQRNLFNILDEQDILLHHPYQAFDLVIDFVRQAASDPNVLAINQTLYRTHSESVMVD
                     ALVNAAHSGKEVTAVIELRARFDEESNLKLANKLHAAGILVLYGVVGYKTHAKMTLVV
                     RRTHGKLKRYVHLSTGNYHEQTAKRYTDLGLLTSEPTITSDAQLIFQQLTGLGKVVKL
                     KALSHSPFTLQKTLLQFIEQCSTAATGGIDTEIILKVNGLTDKVMIQALYKASQAGVK
                     INLLVRGVCCLKPGLPGVSENIRVLSFIGRFLEHHRVFYFRINEEEHYFCSSADLMER
                     NLYNRIEIMFPIYDEGCKKRIKNEIIKNYLKDNNNVWEMQSDGTYKHLMQNGGCAQEK
                     LIALYEEEDKSI"
     gene            complement(14665..15870)
                     /locus_tag="I9760_000749"
     CDS             complement(14665..15870)
                     /locus_tag="I9760_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAT5042536.1"
                     /translation="MKRIFFVVFLLCIETACNTAKNNPLPAQLADKALVPGFKEVRTE
                     FTRSDDPIIKKQSLERVKQLAKAYPDTMFHQKGSFSLLIISGGGDYGAFGAGLLNGWT
                     KSGSRPTFTTVTGISTGALIAPLAFAGPKYDATLKKVYTTITTKDVLSNKPVLWTLLT
                     GGSALKGTKPLQDLIARHVDEKLLDDIAKGYYQGRRLYVGTSNLYSRKLVVWDMTRIA
                     ASKNPERVQLFRKILLASASIPVVMPPVYFDVMANGKPYTEMHVDGSTTFAVFLSRLG
                     SDIKTAKKHFKLKQKPNVKIYVIRNNQNQFHYKVIKPKLLVIGKRAIESVTASQGAAD
                     VIFIYLCALIEGVDFNLANVPNEFDSNSEELFDTQRMNQLYQIGYSKAVKGYPWQKIP
                     PDVRALHGD"
     gene            complement(15946..16479)
                     /locus_tag="I9760_000750"
     CDS             complement(15946..16479)
                     /locus_tag="I9760_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HAT5042537.1"
                     /translation="MIKTFIEIIYSFGKIGLISLGGGNSMLKLLEYEAVTYRHWIGKE
                     EFVGMLGSSFLFPGLTGIKLAALIGYKAAGITGLILAIICLNLPGLIMALVGYQWLNS
                     HNGPITRKIMISVQYGALALLAAATFSVVQGVIDVYYSIPMAICCILFFLALAFWNLS
                     PFYGFLAFIAICFFVVR"
     gene            complement(16476..17096)
                     /locus_tag="I9760_000751"
     CDS             complement(16476..17096)
                     /locus_tag="I9760_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042538.1"
                     /translation="MQRLVWNFEFSSEQTTPLTTLGHDAQDNLKWEKRYFWPTDQIIR
                     LNTIDSSLLDLANYQKKHKEDYYYLLPDSDHNIKQRRNELLYKPVIKQSGSALGFGSK
                     IHLETPQTLPCHGDAGQLNRQELLKKIKEKGIVVFVKKEAFVFKFPTTPTVKLELARL
                     EVKDKVYFSACIEGRSLNLVETISVLLFGTQPSFEYVTFLKKTLKI"
     gene            17272..19710
                     /locus_tag="I9760_000752"
     CDS             17272..19710
                     /locus_tag="I9760_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT5042539.1"
                     /translation="MAIVFFLIYLAFTLVVLSRRINPLVWEIGSIIYLIPATFYFGFP
                     WIIGLFIWFFIIAAVLVVRVEPLRLFIGDYLYKTAGKSVPKLSKTEEEALNAGDTWLE
                     QDIFTGKPDWQKLSAVSTELTAEEQAFLDNETHTLCSMLDEWEISQEYDLSPKVWNYI
                     KEKGFLGLVIPKEFGGKGFSARAHSDIVMKIATRSRVAAVTVMVPNSLGPGELINYYG
                     TDEQKAYYLPRLAKGVEIPCFALTEPGAGSDATSIQSEAIVVKKKIDGKSVLGLNITL
                     DKRWITLAPVATLIGLAVNLKDPDGLLNGEGEEGITCLLIPRDTKNLEIGNRHLPADQ
                     PFMNGTLRGKDIFVPIDTIIGGQKKAGSGWQMLVECLSIGRSISLPALGAGSSSISYL
                     TTGAFARIRRQFNVEIAQFEGVEEKLAEIAGLNYLINSTRLLTVAAVNEHKKPSVASA
                     ITKYFNTELARITVNASMDVHAGRAIVVGPRNYLNSLYQSVPISITVEGANIMSRNLL
                     IFGQGSMACHPFIRDEFYAISSENKEAFREIIWKHIHYFMQNFAKTICSAWTSGLFIA
                     VPENSMKREYKKLARLSYAYSWLADLSLIYLGGDLKRKERLSARLADGMSYLYMAMAA
                     LRNVQLNQDHPDEQLHARWAVSYCFYHAQKSMIALCHNFPSKFLGFIARVVAFPFGQT
                     MRYPSDKLDHQLARLMTGNNHYRDRLKKSVFLSGDPKQPVDRMEHALQLIIEADGLSK
                     KVSDLKRYKWSKLKEKLKEKVESGQLSQQEMEELLAVEDARWDAILVDEFTFDSMKKK
                     TLHSVIEKMKSPLA"
     gene            19824..20516
                     /locus_tag="I9760_000753"
     CDS             19824..20516
                     /locus_tag="I9760_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0294 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042540.1"
                     /translation="MPKYTLPTRDALLKAMQVGETSIEAAEYMATRFEQILTKAKLLP
                     ECNDMLEKIKEYAQFVKFKLLSSAQVWSGQERPTSDYQNTQENKAEFLASHLEGLPSG
                     LKLEVAIGDDAKILRGFSSNGKMVEGDQLKTMDGLLEGWLAKNSLAISGGAVVKIDNT
                     GNQTKVDPQEIRQLINDSEKGVAKYFADKGVGMEVAQRTYQEPKALETKREEIRQEIE
                     SGAEAPTTQSIR"
     gene            complement(20553..21215)
                     /locus_tag="I9760_000754"
     CDS             complement(20553..21215)
                     /locus_tag="I9760_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HAT5042541.1"
                     /translation="MKTAMILAAGRGERLRPLTDKMPKALCTVRNKPLIEHHIINLAN
                     AGFERLIINHAYLGGQIRQYIGNGKQWGLNVIYSPEPPGGLETGGGIVNALPLLGEEP
                     FLTVNADIYTDFDFAKLQLKNIDTFHLLLIPKNPSLLHHGDFGLINGSHLTNTNQAYT
                     FSGIACYNPKVFANCRQGRYSVTPLLRKYVEQNSATGSVHSGIWYDIGSFDRLQAANQ
                     FT"
     gene            complement(21212..22189)
                     /locus_tag="I9760_000755"
     CDS             complement(21212..22189)
                     /locus_tag="I9760_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HAT5042542.1"
                     /translation="MHERENALKEWLAQTIQQKDFVLLPLAGDASFRRYFRIQYNGLT
                     QVVMDAPPEKENIEPFLHIANVLDKIKIPVPDILAINKREGFLLLSDLGDQLLLNKLN
                     HETVDNYYNQAMNLLLQIQKCPTDDPELPLFDKSFMLKEMNLCLEWFLKAYLSLDLKQ
                     DELQLFQHTIEWIASEVATQPRVFIHRDYHSRNLMLVKNKDSCLATIDFQDAMLGPVT
                     YDLVSLLKDCYISWPREKVLEWVTYFHEQSPLASIYSLTDFIRAFDLCGLQRHLKVLG
                     VFCRLYLRDNKAGYLGDLPLTLKYALECAETYEELHPFFNFLQKRVYLP"
     gene            22432..24951
                     /locus_tag="I9760_000756"
     CDS             22432..24951
                     /locus_tag="I9760_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HAT5042543.1"
                     /translation="MAIGITACVLSLINYQGLAYSAALINEPIQACVIAREVDLTDDI
                     RTKFAQCLGWQADQSSPVCLGFYKPIAVTPLASPDEVRILADTASFYRTQRSTLSGHV
                     EMQQGQRVVNAQTAYVYRDPKTNEVTKIEFLNHVRYLEPDRMMIARKAVVYPQDKSGE
                     VEDVLYRFNTNRSNALLPAWGRASLIKRFANQDYFLKEATYTTCAPQDKAWAIEAESI
                     SIDNEKGKGIARNAKLRIHEWPVLYTPYLSFPTNRDRKSGFLMPIVGYSNVGGADLGI
                     PYYWNMAPNYDMTLVPHLYTKRGLMLGGQFRYLTSKSTGTFNGNFLPKDKAFGRFLQD
                     NEVEFPQIRGLSTNRWEVNFVDSTQFLSDLQLNVNFQQVSDDYYLQDFSTNLASVTQR
                     QLLRQADLTYTTENWTFRGMGQSYQTLHPINEIPVSPVYERLPQLMARGYYDDLPFNA
                     QFNILGQYDQFHWPNDSWNIALNNMPQGPRFHLNPILSVPMMKPWGYVTPSVQFVENY
                     YDISRNYTWGTSRANYNLTIPRYSLDGGLYFERDLHLKGTYYIQTLEPRLFYLRVPYY
                     NQTLIPVYDSGFMIFNVDQLFRTNRFSGFDRIGDANQLSYALTTRWLEDESGAEKANF
                     SIGQIKYFSERRVQLCQSPTGFCTDNPDTFGNLSSTFGTSPVASRAVYKFNPAWGITG
                     DYIWDPATRATNNADLNLHYQPARNAIINGGYSYLVNGDVTQVRNNDTENNALHQAIL
                     SAAWPLSEKWSGIGAYSYNISKNYSMMSFLGVQYDSCCWAMRILGGRIFRSLNEEFEP
                     QYNNNIYLQILLKGLGSVASSDPSGILNTYIPGYYDPFRRR"
     gene            25127..26416
                     /locus_tag="I9760_000757"
     CDS             25127..26416
                     /locus_tag="I9760_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone SurA"
                     /protein_id="HAT5042544.1"
                     /translation="MFKRIALVCALFSGVCFAEGKQLLDKVVAIVNDNVITSSELNAQ
                     VELSKKQIIAQNMQMPDESVLRKQVLQHLIDVDLEMQMAKQNGITIENAEIDEAIEKI
                     AASNHLNLSQMRDEITKQGISWQEYRQNIRKEMLISRVQQKAVGKDIIVTNEQVEQYL
                     KTSGRIENSNLTYHLKNIVIPLSEEPTTKQLQRAKIEAENLLNKIKKGEDFSRLAIEE
                     SSGEFALEGGDLGERHLAELPEVFAKEVVHMKVGQVAGPIRAGNGFHLIKLVAVGGEN
                     QRHVITQTHVRHILLKPDASMVPSEAIKQVNNIYRQIQSGKDFALMAKQYSLDAASAV
                     KGGDLGWVNPGELVPEFEKTMNSLPLHKVSKPVKTQYGWHLIEVIARRQKDDSEAFKK
                     QQVRQFLQQRKFVEAVQNWQQHLRSQAYINIVDKDLA"
     gene            26413..27387
                     /gene="pdxA"
                     /locus_tag="I9760_000758"
     CDS             26413..27387
                     /gene="pdxA"
                     /locus_tag="I9760_000758"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAT5042545.1"
                     /translation="MNPLLISSGEPAGIGPDLCLALAETDLPVVILGDLSLLEARASE
                     LNLSIKFLEYSPHQSFKKKAGYLTVWPVPCAAPVISGELNPQNAAYVMELLTLGASLC
                     SKGEFSALVTAPVHKANINAAGITFTGHTEFFADFFEVETVVMMLACSQMKVALVTTH
                     LPLRMVPDAISSLLIIKVIQQLHHSLKHDFGIQSPKINVAGLNPHAGESGYLGREEIE
                     IITPALNTLKNQGIDVLGPLPADTMFITNHINHCDAYVAMYHDQGLPVLKYAGFNEAV
                     NITLGLPIIRTSVDHGTALELAGKNKANPGSMLAAVKMAKDMALTRIK"
     gene            27389..27880
                     /locus_tag="I9760_000759"
     CDS             27389..27880
                     /locus_tag="I9760_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAT5042546.1"
                     /translation="MSLISLIAAIDETGGLGINNQLLCYLPADLQHFKSITMGKPIIM
                     GRKTYESIGKPLPGRLNIVISQSIQSIPGVVVVDSLGRAIKETADASEVMIIGGAKIY
                     SQAISMANRLYITRIHHQFTADVFFPKINELAWCCRSKEYRQHDDKNQYDMTFCVYER
                     ISI"
     gene            complement(27887..28432)
                     /locus_tag="I9760_000760"
     CDS             complement(27887..28432)
                     /locus_tag="I9760_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3421 domain-containing protein"
                     /protein_id="HAT5042547.1"
                     /translation="MSKLVYLLLMIFFLNPAWANTHYHQHPVSPLANALRTGTDTNGN
                     ALYLCRAKLFNSIQPGKTWAGYGRCNVPYGGKEYVLSQFTIPNQNEFGRYSWEPNVEH
                     ALLMGKDTNGNPLFVCQSNFNGSIQPGKTWPGYSHCNISYGGREIITDNYRILSRPTE
                     IIVRTQPPVHRHPSQNRHYHY"
BASE COUNT         8557 a         5190 c         5906 g         8884 t
ORIGIN      
        1 tgtaataaaa cagaatcttt tgcattagcg agtaaattag taagaatttg aaataacttg
       61 gatttatcag tgataaactc attgatatcc ggttcaatct caatacggac atcaatttga
      121 tgtttgtcca atatgactga ttttaccgca ttaagaatca catcatggat tgaaattttc
      181 tctgttaaat tcgataagcc gctcaatgat tgctgcatgg ctatgatttc tttaatatga
      241 ttaatgttag tttccagact gttggattcc ttcatgtttt cttcatattc ttcatgcagg
      301 gttttcgata acgcgtccaa atagggaaga attaacttac cctttggatt ctgtaacaag
      361 tattcgctta attgattgat gttttcgtca agcatggttt ttatagcgga cagtttttta
      421 taatgaattt tggatatatt ctttttcaga ataatggcag aaacattgac gctgtttaat
      481 atattgccca cattatgaag tatcgaggtg gctacttccg acatcccggc aagtcgagct
      541 gttgaaacca gtttattatt cagttcatga acttgctctt caataatttt ccttttggag
      601 atatccatca aaaccccgga aatttcgttt ttatgaggat tggctcttgc tatcgttcgg
      661 taccatttgt aatcattgtc tccgtttctt actctaagct cataatcata atcttctttt
      721 tctttgaggg ccttttcgac cacatttttt aaccctgctt tatcattagg atgcacaaga
      781 tctaaaaatg aagaatagtt tagtgccaca ttactaggcg gcaaacccag aattttaaat
      841 agtcctttgg accatgttgc ggtatctgtg tcagcgtcat aatgccaata cccaatttgc
      901 gctatatcct gggcgtattc gagttttgca ttgatatccc tcatttcttt agaagaaata
      961 tcaatagagc gctctaataa atatcggtct tgatcgtttt cgtagtatgt tttattgata
     1021 tgtgcaataa attgagtcca actctccaga ttttgtggta atccatcagc agtaaaacca
     1081 aattttttta actgtcgctg caacaaccga tgcatttctg tcattattac tgaattcctt
     1141 tttggtttat ggactctcta acagggtcgt aatggtcatg gtttgattat gcaattcgca
     1201 ggtacctgtt gccagaggtg ataattcacc gtaagagtag aagccaattt gtatcgattt
     1261 gggaggcagt gattgtaatg ttgtttcggt ctcttcctcc gtaagttcac ctaataccaa
     1321 tcttctgccg acacaactaa tatcgattgc caaaacaggt tgtgtattca agtttccatt
     1381 tttaaaaagt ctatcattag cgttttctcc ggcttcattg gcgctgacaa tcaggcggtc
     1441 aaaattggca cgcattaatt gggcatagta tccttgaggt aaatcgccag caaaaatgag
     1501 ggattgctct tcttcattga ctcccagaat agtgcgcaca atgtgctgag aatctggtgc
     1561 ggatgggtta cggatagcaa gagggtaaag caagcctgcc gctggtaatt cagaagctct
     1621 gtcaccaaga taggttttat agagttttag cgcgggctgg ttgtccagtt catagagaat
     1681 atttgcttta gaccgtgtaa tgactctttc tggtccaaaa atatcccaac ctcctcttga
     1741 accatgtcct acatggattt gatcaccgta aaaccctact gccccaatgc aatggtctac
     1801 aatttttcca tcataaataa tccaggtatt tttaaaatcc gcaccatcta ctgctagacc
     1861 ccctgtaata ataacccctt tgccagcatg ctggtttaat cctttaatta attcggaacc
     1921 gttgacattt aagccatcgg acaaaacaaa tatgctttgg ggattttgct tttgatgcaa
     1981 tgattttgat aaatgaactc ctgcctcata agactcttca gtgcttttaa cctctgtttt
     2041 tgccaattga agtttcgttg actcaaattg aactactgca accgaaatgc tgttatcaaa
     2101 aatattaggc ccaaaaattt ctcctgcagt ggagcatcct atgatttttg attttttata
     2161 ggcttgagcc aattcttcaa ttggtttgct gtttgcccta aagatggggc tggcaaaaac
     2221 taaaattaag gtattttcgg agtcgagttc aggaaatgtt gggatactcc atccctggtt
     2281 ttgaatgtat tgaaatgatt caattttcat catattgctg tgacttggat ttatcttatg
     2341 attaattata gtatatgttc aaaaggatca atgaagtgga ggttctgtct attgcaatat
     2401 tagtgataaa aatctgttat atgaacccga atgttttttg tgataaagcc aattattttc
     2461 ataaaagatt cattcctgtt aattatcgcg attttgccat tttttaaagt tgcttagcag
     2521 gtcattaatc aagcggtcac tgattctttt tttcagataa aaagcatgaa atgagattgt
     2581 tagttcaagg tcttttattt ttaagatttt taatttttct gacagtaaac tatcaggcag
     2641 atagctccag cctaaaccgt actctactag agattttatg gcatcgatag tactcatttc
     2701 aaaacccagg attagtacca gttgattttt ttcaaatagg gtatcaagct tgtcacgcaa
     2761 tgctgctcct ttctcagtta agatggccgg gtactctgaa agttctgaga gtgaaagctc
     2821 ggatgttcta tgggcaagct catgatttgc tgctacaacc aaattcagtt tttcctccca
     2881 gagcaaacaa ttgcccaata atttttcaga tacttcccgt gccgggcaga ttaccagatc
     2941 aaccgtgccg tggttcagaa tatactcgac ttccttttca ctaatggttt tcagatgcgc
     3001 ttgaatggtc ggatgggttt cctttaagta tttaaaaaag cctggcaata cgtagtgaga
     3061 cacatacacg gaagccccta tgatgatgct ttgcgtgaat tgttgcgagt ttttcagggt
     3121 gtgaaccata ttttgatgtg cggaaagcat ttgcctggca taaggaataa gctgcttggc
     3181 tgtttcggta aaaattaatt tggaaccctc gctaattatc aaacgtacat ttaactcgtc
     3241 ctctattttt cgaatgcgtt tgctcatagc tgattgagtg acatgcaatt ttttagctgc
     3301 caaggtgaga gaatggtatt cgacgacggc gagaaaggat ttgatgtctc taatatccat
     3361 agaatgcttg cctttttata agaagtatag catagcggca ctaagcgctt tctaaacaag
     3421 gacggcagat gagtataatg ttctaagcag ggggcgaggg atcatgcggc attttaaacg
     3481 agggatacag gcagagagga gcagtgaatt gaaaacagac aatcattctg ttgattgcca
     3541 aaatttatgt gacaccgggt taaaaattta ggagaaataa tctactttaa aaaaaactgt
     3601 atgatcaaaa aggccagggt taataaaatg gaaatcatcg ggaaaacaat agggaatgaa
     3661 atggcgtttg ttggtttctt ttttgtcagt ttgattttaa ttagcgaggc atgaatgatg
     3721 ataaaaatac acaatatgat actgctggtt aatttagcca gggttgtaat agggagggca
     3781 taggcaaaga gtaaaataat aagagacacc agtaaggtcg agttgactgg tgtttgcgtt
     3841 ttggaataaa caaaggagaa tatttgaggg gcattttgtt gattagccat accataaata
     3901 agtcgcgaag ccataataat ttgcaccaga attccattgg taatagaaat tagtgcaatg
     3961 attgaaaaca acaaggggga ttgtccctga agcttaataa tttcaataag gggcatgtcc
     4021 gttttgacta aagcattgga tggtatggtc gaaacgataa cccaggcgac tgcaacatat
     4081 aaaatggttg cggatattaa agcaagaaaa atagcttttg gaagagtttt ttcaggattt
     4141 attgtttctt ctgctgtatt aaccatatct tcaaaaccaa taaaagcata aaaagcaata
     4201 aaagctccaa taagcacccc atcaaaagaa tgaggaagag agaaggattg gatgtcgata
     4261 gaagcgaatg aataacgacc gtaataaata atcatcaata atccacccac ttccatgagc
     4321 gtcatgtaca ggataagagt cgctgattca cgaatacccc aaacagcaac tcccgctaaa
     4381 atgacagtta atatagtgat gatgagagaa ggtggtaagg gtaaaaacaa ttgaaaatat
     4441 ttgacaaaac catgggatag ggtggctgcc gagatcacac ctgttaaaac cacaatccaa
     4501 cctattaagc cagatagcca tttttgatgg aatgccctcc gcacgtaaag agcagagcct
     4561 gcactaagcg gtaacctgga gcttaattct gcataagaaa tggcagtaaa aagagcgatg
     4621 atggaggcga ttaagaagga tagaggggta aattgtgccg cttcttttgc gacctcacca
     4681 acaagggcat aaattcccgc gcccaatata gttccaagcc cataaaaggc cagcagtgtt
     4741 aatgataaag tccgttttaa gtgcatcgcc cccccatttt atctggagat ctatagtcgt
     4801 agaacaagtc ttcttttttg tttgcttact tttttatttt tcgccggtta ctaaatagat
     4861 agtgaaatta aattgatatt caccattata tttggcttgg cattcattat cgtaatattg
     4921 aaccatagct tcgttgattt tttgtatctc ttcattcggc aattgtcctt gataaaaagc
     4981 aattccattg acgaatttcc tgaaaacatc cagggaaggg agggtaatgg attgtcggca
     5041 tagctttact tttagctcct gacagcttaa cgactccagt tttttttcca gattctttaa
     5101 gtgagaataa tctacgggag ggataaaatc atgcaaggat gcaaattgcc ctgatttttt
     5161 taaagcataa taactcatga taaacggatc gtctcctgct ggaaaaagag taaggaattt
     5221 tcccccaggt ttcaatgcgt taacgatatt taaaaatgct ttttgaatat tggcacaagc
     5281 ccattgcaaa caccaaaaag agacgacata gtcaaattgt aagtggaagt ccattgtgag
     5341 tacatcggct ttctggactg aaaagttagg atattcctta ctcacgtctt gggctaaatg
     5401 taacatattc tcggaagcgt ctatacctaa tacagagcct tgaggcactt tcataagaat
     5461 gttttttgta aaagcaccgt taccgcagcc gatatccaat actttgtcgg cgggcttcat
     5521 gtttaggtag ggtaaatatt cttcagccac agtcgcttga atgtaagatc caatggcata
     5581 gtcattggcg ggccactcac gtcttgacat acttattcct ttcttttgag atggttattt
     5641 attccataat tgttcaggtc atcattgttc atcatacatc atggacagtc cctaaaacag
     5701 atcatgaatt cttttatgaa tagtattttt attgagatga atgacttaaa tcaagtatag
     5761 tgagcgctgt aaaaaaatca ggatgttttt acttcaaact atgaaaagga gtcaggtatg
     5821 ttcagccaga gtcagcaaaa aattgtttgt gtgctttatg atgatcctaa ggggggattt
     5881 cctccaaact atgcaagaga atccattcca gaattgaaac aatatcctga tggtcaaagt
     5941 ttacctaatc ctgattcgat tgattttatt ccaggtgaaa tgctaggttc tgtgagtggt
     6001 gaattgggac taagacaatt tcttgaaagc aatgggcatc aattggtagt aacctctgat
     6061 aaggatggct ctgattcagt atttgccagg gagctaaaag aagccactgt cgtcatttcc
     6121 caaccttttt ggcccgctta tttaactcgt gatcgtattg aaagtgcacc taagcttaag
     6181 ctcgctatca ccgcaggtat cggctcggat catgtcgatt tgcaagcggc catggaacat
     6241 aatatcaccg tttgtgaggt cacttattgt aacagcatca gtgttgctga gcataccgtg
     6301 atgatgattt tagccttggt gcgggatttt attccacaat acaatacggt aattgatggc
     6361 gggtggaata ttgcggattg cgtcagtcgc tcttatgatt tggaaggcat gcaagtggga
     6421 tgtgttgctg caggccgaat tggattagct gttttaagac gcttaaaacc gtttgctgta
     6481 aaactgcatt ataccgacag gcatagacta ccagtacagt tggagcagga actgaatttg
     6541 acttatcacc cctctgttga atccatggtg aaagtttgtg atgtggtatc gattcattgc
     6601 ccattgcatc cggaaacaga ataccttttt gatgagcgat taataaaaca gatgaaacga
     6661 ggctcttatt taattaatac cgcccgtgga agaatatgcg atcagcatgc tgtagcggat
     6721 gctctggaat ctgggcattt ggctggttat gccggagacg tttggttccc acaaccccca
     6781 gccaaaaacc atccttggcg atcgatgcca aatcacgcca tgacaccaca tacatcagga
     6841 actaccctgt ctgcgcaagc gcgatatgcg gcgggagtca gagaaatatt agagtgttgg
     6901 ttagggaaca aaccaattag ggaggaatat ctgatcgtgt ctcaaggcca tttagccggg
     6961 gttggttctc attcttatag tgcgggtaat actacttcgg gaactgagca agcagcagaa
     7021 ttggtttgat tccccgtcgc ttatgctgtc ttatgtcttt ctgttttcat tcaaagtagc
     7081 cgctgccttt aaagacagcg gcagttcagg gaaaaggggc aaacagtgga tgatttaaaa
     7141 tttaaataaa ttctttggaa ggtctggttt taaaccaaca tcgtcttggc caaatgaaaa
     7201 gaaacctgga ttgtagacag ggtgtgtttg ctcaaaatgc tctagaaagt cgtctgcggc
     7261 tccttcgtta tcccaagtgt tatggcttga gtctgtatat actgtttctg taggttgatt
     7321 ggatgtttct tgtctaggtt gaggtacttc ttgttcctga ttctcatatt ttcttctttt
     7381 tgtattggta tgaatttctc tctccgaaac gattgataga tcaaaataat ctctgtgatc
     7441 aagtagcgaa tgaatacagt ttgccatgtc tttttcagat gatgggtcac gctctatagt
     7501 gcctccttcc cgcactgtat gggtaaaaca ttgaccttcc tgattgattg aaaaataatt
     7561 gacaagtcgg ccgaatctct caaaaacgta gcctatttcc agttgttgat taccctttga
     7621 taagggaaag gccaagttca accagggatc cattaaaaac tgttcattga ctattagtat
     7681 aaaatgatta ttaaaggtcg ctaatcgaag ggagtgaatt aagctttctg gtttttgtgc
     7741 ctggtgtaac agaagtgcaa aaacaaaaaa agctttttcc tggcaattac caaaaccaga
     7801 gcctctgcta tacagagcaa tcttcagaat tttatttagc caggtcaaat cagttctttc
     7861 cagagtttca tcagcgaaaa tttgagttag cttaaccagt gaagcatcgt cttcattatc
     7921 ggtaggtaaa tcacaggaac tgatgttttg tgccaattga ttgccaagca gaatcagctc
     7981 ccagagtttt ttttcttcag cattgagatg gaatttatca ttaactgtcc ggacttttgc
     8041 tttaacttcc tgaacttgag aatgagtaag ggcggcatac tgattcggct tttttgtttt
     8101 ttcagaagat ttgcagtgaa gttctctttt tttggagcgt ctttcttgtt gctgattaag
     8161 tgctttttct ttcatttttt gaaaatcacc taaaaggctg tccataaata attcctgtct
     8221 attttgatca gtcgctttgg cacaggattt tgtgaacatg ttacactctc tttataatag
     8281 ttaattacga tatgacaagt tgaacctctg atgataatga gctggactgc tgtaaaaaca
     8341 gagcctggtt cactgagact gctattttat aagttaatta ttaagataac attaattttt
     8401 gcatttaaat tggcctgatg gggtgtattt ttgtcaaatt gtaagcgatt tttgatttaa
     8461 aaaaggtata atagatgaca aaataaaaac cacctgtggt tatcattgga atattgatga
     8521 gcaaagaacg aattaagaaa acagcactgg agattgtcaa atacattcat ggcaacataa
     8581 aagcgtgtaa tatccccaat caaaaaaaag aaaataagtt ttttgcgcct ttggtttatc
     8641 tctgtcaaac agaatgcgat aagattttga gtaacaccaa aatcagtttg ttggagcgtc
     8701 ttttaaaatg cgcaaaaatt atcggtgatc tgcaatcagg aaattgtatg cagcaaacct
     8761 ttttagcatt tcaacgttta ttgatgcgat tgattgagga taagctatct aatttttcca
     8821 cttgcattcc aatttctgtt atgaccattt ccaaccatgc ctttttaatc attgataacg
     8881 atatcgtgtg tgatccctgg ttaaattttg tgggagattt gaaagattat tgttttgcaa
     8941 acatgaaaag gaaagagtat tttggaatac gttccgactg gacttgtttt accaattcag
     9001 aggtctatga tgaagactct actgaattta caatccaatt tgtaaatgct ttaggggtag
     9061 ggatatcggg tttcagtgaa attaagttgg caaatcaatt gatgcaatca tcgagtatgg
     9121 cttctgatct tggagaggat cctgtgattt ctatccccaa agagtttcat cgaatttgat
     9181 ggagcgctca tcttgattgt aatgaactct attgctgttg tttttgttca agttctggtt
     9241 cgatttaacg gtaaatatat tgtacttgat aatgatgggg gagaggatga attttttaaa
     9301 caataaaatt gtaatgataa ccggcgcgtc cagcggcatt ggacaggctt gcgcccgact
     9361 ttttgctgca caaggagcac gactcatcct ggcggcacgg cgagttgaac gtttacatga
     9421 attatccagt gggttaaaac atcataataa tcaagaacat tatattcttc ctctggatgt
     9481 ctgtaagaaa gatttggtga gacaacaaat cgaatctttg cccggtcagt ggaactctat
     9541 tgatgtgtta atcaataatg cgggtttggc tctggatacc ttgcctttgc atcaaggaat
     9601 attggagcat tgggacacca tgatagatac caatattaaa ggattgcttt atattagtcg
     9661 gctaattctc ccagggatgt tagagaggaa tagtgggcac gtcattaata tcggttccat
     9721 agcaggtcat gagtgttatc ctaatggaaa tgtttactcg gccaccaagc atgctgttca
     9781 tgcgatatca aagtccatgc gattggatat gttaggaagt gcagttcgag tgactgaaat
     9841 tgcaccaggt gctgttgaga cagagtttag cgaggttcgc tggaaagaca aacagcgagc
     9901 tatcgatttc tataaggatt ttcaaccgct tatggccgaa gatgttgctg atgctgcttt
     9961 gtattgcgct acaagacctc tgcatgtcga tattgagcaa atgattatta tgccaacagt
    10021 tcaggcttct gcaaatcatt tgtcaaggaa aggcagataa tcatcaggtg aataattgtg
    10081 caaaaatttg ccaaaaatgg ggctgttagt ctattattta aaatataggt gatgactgat
    10141 cctggctgaa ccagtaagta gtgccagatt aggaggtcgt tttggagggt ggtgtgtaag
    10201 cctacaaagg gtaggaaacc tgaagctcta cataaatctc cacagtgtct ttcagttgcg
    10261 gctcagaaac cgggaagtcg ttgcctgtac ttaaactctt ttctattctt tgtataaact
    10321 aattttttct tatcaaagaa tgaattaagt cgaacagatc atagacaaac ccacaatcgc
    10381 tttgttaaaa atgtttttaa ttcggtcatt tgttttaatt aagctcgctc attaaaatat
    10441 tttttaatgc cccttatggt tttgagtaaa tccagttaaa gtcaatcccg atatcctctg
    10501 tttaaaataa agagcaattt gagcaattaa taatattatc ttataattaa agaacaacta
    10561 ttttgccagg aagcaattat gaacaagtta attgttgcgg tcactgctgg tgcatcatta
    10621 ttgctgctaa ctggatgtgc ggttgatggt gtggctattg atgatgtttc ttacgaatca
    10681 ggttatagca atgactacgt ttattcggtt ggctattata gcaatcgccc ctattggggt
    10741 aacaactact attcgtcacc ttcttttgta ggcccttatg gatattgggg tggagattac
    10801 gatttctaat ttccagagaa gacaagcctc caaaccaaat gaaggctatt tctgttgata
    10861 attactcttt agcgcgagac acatattcgc ctttgcgcgt gtctacctta atcagttcgc
    10921 cagtttgaac aaacaatggt acacgaacca cggcacctgt ttccaatgtg gctggctttc
    10981 cgcctccgcc agaagtgtct ccttttaatc caggatctgt ttcggtgatt gccaaaatga
    11041 caaaattggg tggagtcacc tgtataggtt cattgttcca tagagtaact acacaaacgt
    11101 cctgttcctt taaccattgt gctgcatcgg ctaatacgtt ttcagtcacg gcatattgtt
    11161 caaacgtatc tggaaccata aaatgccaat gctccccatc attgtataga tactgcattt
    11221 ctacgtcagc cacatcagca gatggtaagg tgtccccaga tttaaaggta cgttccacaa
    11281 cgcgcccggt ttttagattg cgaattttaa tacgggtaaa tgcttgacct tttccaggtt
    11341 ttacaaattc acagtcaaga atgctgcacg gagcatcatc aaccattact tttaagccgt
    11401 ttttaaattc atttgtgcta tagactgcca tttctgctcc atagttgaga ttttgctgta
    11461 ttttcgctaa agttcgcaca gtttatcaag tctgtaactt taatgcgaga tacctctttg
    11521 agttggcaaa aaattttggc gcaaggattt acttcaacga ctgatttatt agatttcctg
    11581 gaattgccga gatctgaagg aaacctattc gcggaaaaac aattcccaag ccgtattcct
    11641 ttaggttttg ctaagcgaat gcaaaaaggg aatcccaaag atcccttgct tttacaggtt
    11701 cttgcaaaag aagatgagct tagcgaagca gatgattatg ttatcgatcc cttaagtgag
    11761 agtaataccc tgattaaagg tttgttgcat aaatatcacg ggcgcgtttt gttaacttta
    11821 accggtgttt gcgctgtgaa ttgtcgttat tgctttagaa gacattttcc ttatcaagcg
    11881 aataatccgg gccgccgggg atggaaagag gtgtgtgcct acatcgcaaa tgattccagt
    11941 attactgaag ttattttaag tggtggcgat ccattgctcg ccgccaattt ggttttggaa
    12001 gaattactcc aatctttgga agaaatttct catatacata ctttaagaat tcatacaaga
    12061 attcctattg ttttaccgga gcgtattgac aaaggattac ttgatttatt aacaaatacg
    12121 cgatttaaga aagtgattgt tgttcattgt aatcatccgc aagagttgga tgagagtgta
    12181 ttgcgggcgt gctctgattt gaagaaagca gcatgttatt tactgaatca atctgtttta
    12241 ttagccggga ttaatgatga tgcagggatt ttatccaagt taagtcatgc tctttttgat
    12301 tacgggatta tgccttacta cttgcatttg ctcgataaag taaagggttc ggcacatttc
    12361 gatatgccgc ttctcagggc tcagtcaatt tatcatcaat tacaatccct tgtgccagga
    12421 tatttattgc ctcgcctggc acgagaagag ccgggaagat ccagtaaaac cttgctgatt
    12481 taaagagctt gatatgttct gtctatttat ggttttattc aatagccatg tttccactgt
    12541 tatttttctg actaaatcga tttgtcctct tcctcataaa gtgcaatcag cttttcctga
    12601 gcacagccgc cgttttgcat gaggtgcttg taagtcccgt cactttgcat ttcccaaaca
    12661 ttgttattat ctttaagata gtttttaatg atttcgtttt ttattctttt tttacatccc
    12721 tcatcataaa tggggaacat gatttcaata cgattgtata agtttctttc catcagatcg
    12781 gcactggagc aaaaatagtg ttcttcttcg ttgatgcgaa aataaaacac tctgtgatgt
    12841 tcgaggaatc tgccaataaa agataagact ctgatgttct cagacacacc aggcaatcct
    12901 ggttttaaac aacatacacc acgtacaaga agattaattt taacgcctgc ttgtgatgcc
    12961 ttgtataatg cttgaatcat cactttatca gttaatccat tcactttcag gatgatttca
    13021 gtatcaatgc ctcccgtagc agcagtactg cattgttcga tgaattgtaa aagagttttt
    13081 tgtaaggtaa atggtgaatg gctcaaggct tttaacttaa caacctttcc aagaccggtt
    13141 aattgttgaa aaatgagctg ggcgtcagaa gtaatagtgg gttcactggt taataatccc
    13201 aaatcagtgt atctcttggc tgtttgttca tgatagtttc ctgtgcttaa gtgcacatat
    13261 cgttttaatt taccatgcgt tcttcgaacg accagcgtca ttttcgcgtg agttttatag
    13321 cctactactc cgtagagtac caggatcccc gcagcatgga gtttgttagc caattttaag
    13381 ttggattcct cgtcaaaacg ggctcttagc tcaatgacag cggttacttc tttccctgaa
    13441 tgggcggcgt taaccagcgc atcgaccatc actgattcag agtgggttct gtaaagcgtt
    13501 tgattaattg ccagtacatt agggtctgaa gcagcttgcc tgacaaaatc aatgactaaa
    13561 tcaaatgctt gatagggatg atgcaataaa atgtcttgtt cgtcgagaat attaaataaa
    13621 ttacgttgtg atttggagaa cttgggatac tgtggggtaa aagcgggata attcagatca
    13681 ggtctatcga tgctgttaat agcactcata tatcgttgta aattaacagg gccatcacaa
    13741 taataggtgt cttcatgccg caaatgatat ttctgcagta gaaagtcaac aattttttca
    13801 gggcatttgt tttcaatttc caatcttacg acatggccat aatgacggga aaatatctct
    13861 ctttgcaccg ctttagccaa atcgtcaatt tcttcttctc ttaaaaataa atcgctattt
    13921 cgcgtcaacc gaaatgaata gcatccgctg atttccatac ctggaaataa actattaata
    13981 tgcgttttaa taatggaaga taaataaaca aagtaatggg catctccaca aagctcggaa
    14041 ggcagatgta tcatgcgtgg aatcgagcga ggagcatgta cgacggcgta attgatattt
    14101 cgatcaaaag catctttccc actcaatgaa atgataaaat ttaaactttt attgataagc
    14161 cttggaagag gatgggccaa atcgagagca atagggctta taacaggtag aatttcatgt
    14221 ttaaagtaat gctttgccca taaatgaatg tcatcagtcc attgatctgt ctcaagaaaa
    14281 tggatatttt ctttgcgcaa tgccggaagt aattgtttgt taaaaatatt gtaaagctga
    14341 tcgattaaag catgagcctt atggcttatt tggctgaatg cttcatcagc ccgtaatcca
    14401 tctatggtta gcttgcctga cgacatggca attttttctt tcaacccggc cacccgaatt
    14461 tcaaaaaatt catcaagatt actgctgcag atactgagaa atctcattct ttccaataag
    14521 ggtacgcgtt catcattagc aagcatgagt actctctgat taaacgcaat agcagaaaat
    14581 tctctattaa tataataatc tgggttatcc agagtagtat ccatgaggaa ctccaggtat
    14641 tttcatttca tgttcacagg caatttagtc accatgcaaa gcccttacat ctggagggat
    14701 tttttgccat gggtaacctt ttaccgcttt ggaataacca atttgatata actgattcat
    14761 tctttgagta tcaaacaatt cctccgagtt cgaatcaaac tcatttggaa cattggccaa
    14821 attaaaatca actccctcta taagcgcaca aagatagata aaaataacat cggcagcccc
    14881 ctgggatgcc gtgacacttt ctatcgctcg cttgccaatt accaataact tgggtttgat
    14941 tactttgtaa tggaattgat tctggttatt gcgtattaca taaattttaa cgtttggttt
    15001 ttgttttaat ttgaaatgtt ttttcgcggt tttgatatca gaaccaagac gacttaaaaa
    15061 gaccgcaaac gttgttgagc catcaacgtg catttctgtg tagggtttcc cgttggccat
    15121 gacatcaaaa taaacaggcg gcatgaccac gggaatgctg gcagaagcaa gtagaatttt
    15181 tcggaataac tgaactctct cggggttttt gcttgccgca atgcgggtca tatcccatac
    15241 caccagcttt cgagagtaaa gattggatgt ccctacataa agccgtcgcc cttgataata
    15301 gcctttggca atgtcatcaa gcaatttttc atcgacatga cgagcaatca ggtcttgtaa
    15361 tggtttggtt cctttaaggg cgctgcctcc tgttaacaga gtccataata caggcttatt
    15421 actcagtaca tccttcgtgg taatggttgt atacactttt tttaatgtag catcatattt
    15481 agggccagca aaagctaaag gtgctatcaa tgccccagta cttatacctg tcacggttgt
    15541 aaatgttggt cgagaaccgg attttgtcca cccattcaat aatccagcac caaaagcacc
    15601 ataatcgcca ccacctgaaa taatcagcaa gctgaaagag cctttttgat ggaacatggt
    15661 atcagggtaa gctttagcca gttgttttac acgttcaaga gattgctttt taattattgg
    15721 gtcatcactg cgggtgaatt cagtgcgtac ctctttaaat ccaggcacta aggccttatc
    15781 tgccaattgc gcggggagag gattattttt tgctgtattg caagcggttt ctatacaaag
    15841 caaaaataca acaaaaaaaa tccttttcat aattttcctt taaattaatg ttttcccgaa
    15901 ttttattcaa aaaatggatt aaaagcgata gtgtttgttt tcatattaac gcaccacaaa
    15961 aaaacatatg gctataaatg ctaaaaaacc ataaaagggt gatagattcc aaaaggccaa
    16021 ggcaagaaaa aacaaaatac agcaaatagc cataggaata gaataataga cgtcaataac
    16081 accttgaact actgaaaatg tagcagccgc taacaaggcc aaagctccgt attggactga
    16141 gatcataatt ttgcgtgtta ttggaccgtt atgagaatta agccactgat atcccacaag
    16201 agccataatt aatcctggta aattcaggca gatgatcgca agaattaaac cggttattcc
    16261 ggccgcttta tagcctatta gtgcggccag ttttattccc gttaatcccg ggaataaaaa
    16321 acttgaacct aacataccta caaattcttc ttttccaatc cagtgcctgt acgtgactgc
    16381 ctcatattcc aataatttta acatggaatt gccgccgcct aaggatatca ggccaatttt
    16441 cccaaaactg taaatgattt caatgaaagt ttttatcata tttttaaagt cttttttaag
    16501 aaggtaacgt attcaaaaga aggttgggtt ccgaataaaa ggacgctgat ggtttctacc
    16561 aggtttaatg atctcccctc aatacaggca ctgaaataca ctttatcctt gacttcaaga
    16621 cgagcaagct caagcttgac agttggcgtt gtagggaatt taaaaacaaa agcttctttt
    16681 ttaacaaaaa caacgattcc tttttctttt atttttttca atagttcttg tcgattcaat
    16741 tgcccagcgt cgccatggca agggagagtt tgtggggttt caagatgaat tttagatcca
    16801 aagcctagtg cagagccaga ttgcttaata acaggtttat acaataattc gttacgtctt
    16861 tgttttatat tatgatcaga gtcaggcagt aaataataat agtcttcttt atgttttttc
    16921 tgatagttgg ctagatcaag caacgaactg tcaatagtat ttaaacggat gatttgatct
    16981 gtaggccaaa aatagcgctt ttcccatttt agattgtctt gagcatcatg acctaaagtg
    17041 gtaagtggtg ttgtttgttc agaggaaaat tcaaagttcc aaaccagtct ttgcattatt
    17101 agtatttcat tttctgtttt gcttacgcta gatgatttga tttgttttgg caaggttaac
    17161 ttaaatgttt cttataattt ttaaaggatt attgctcgct aaataaagcg taaaaagtat
    17221 atactggtta ttctataaac ctcttaataa aatagcaaag gaaggtattt tatggctatt
    17281 gtgttctttt taatttattt ggcttttact ctggtagtac tgtccagaag aattaatcca
    17341 ttggtttggg aaattggaag cataatctac ctcattcccg caacatttta ttttgggttc
    17401 ccctggatta tcggtttatt catttggttc tttattatag ctgccgtgct ggtagttcga
    17461 gtggagccat tacgcctttt catcggggat tatttgtata aaaccgcagg aaaatcggtg
    17521 cccaagctct ctaaaacgga agaagaagct ttaaacgcgg gggatacctg gctggaacaa
    17581 gatattttta caggaaaacc tgattggcaa aaattatcag cagtttctac agagctcaca
    17641 gcagaagaac aagctttttt ggataatgaa actcatactt tgtgcagtat gttggatgaa
    17701 tgggaaatta gtcaggaata tgatttatct cccaaggttt ggaattacat taaagagaaa
    17761 ggatttttag gattagtgat accaaaggag ttcggtggca aagggttctc agctcgagcc
    17821 cattctgata ttgtaatgaa aattgccact cgctcaagag tagcggcagt gacggtaatg
    17881 gttcctaatt ccctgggccc gggcgagcta ataaattatt atggaaccga tgagcaaaaa
    17941 gcatattatc ttccacgtct tgccaaaggt gttgagattc cctgttttgc attgacagag
    18001 ccaggggctg gaagtgatgc gacctccatc cagtcagaag ccattgtagt taaaaagaag
    18061 atagatggga aatcagtact tggcttaaat atcaccctgg ataaacggtg gattacttta
    18121 gctcctgttg cgactttaat tggattggcc gtgaacttga aagatccgga tgggcttttg
    18181 aatggtgaag gagaggaagg cataacttgc ttgctgattc caagagatac taaaaatctt
    18241 gaaattggca ataggcattt accagctgat cagcccttta tgaatggaac tttacgcggc
    18301 aaagatattt ttgtgcctat tgataccatc attggtggtc aaaagaaagc gggatccggt
    18361 tggcaaatgc tggttgagtg cttatctata gggcgctcaa tatctctgcc tgcacttgga
    18421 gcaggttctt cttctatttc ttatttaaca acaggtgctt ttgccagaat tcgtcgccaa
    18481 tttaatgtag aaatcgctca gtttgaagga gtagaggaaa aacttgcaga aatagcgggt
    18541 ttgaattatt taatcaactc tacccggttg ttgacggttg ctgcagtgaa tgaacataaa
    18601 aaaccctctg tagcttcagc gattaccaaa tattttaata cggaattggc acgaatcact
    18661 gtgaatgctt ctatggatgt tcatgctggg cgagccattg ttgttgggcc acgcaattac
    18721 ttaaatagtt tatatcaaag tgttcctatt tctattacgg tagaaggggc taatattatg
    18781 tctcgcaatt tattgatttt tgggcaggga tctatggcat gtcatccgtt tattcgtgat
    18841 gaattctacg ccatctccag tgagaataaa gaggcattta gagagataat ttggaagcat
    18901 attcattatt ttatgcagaa ttttgctaaa acaatctgtt cagcatggac aagtggtttg
    18961 tttattgcgg ttcccgaaaa ttcaatgaaa agagagtata agaaactggc gcgtttgagt
    19021 tatgcctatt cctggcttgc tgatctgtct ttaatttatt tgggaggtga tttaaagcgc
    19081 aaggagcgat tgtctgcaag attggctgat ggcatgtctt atctctatat ggcaatggct
    19141 gctttacgaa atgtgcaatt aaaccaagac catcccgatg agcaattgca tgccagatgg
    19201 gcggtaagct attgttttta ccacgcgcaa aaatccatga ttgcactttg tcacaatttc
    19261 ccttccaaat ttttaggctt tattgcccga gtagttgcgt ttccatttgg gcaaaccatg
    19321 cgttatcctt cagacaaact agatcatcag ttggctcgtt tgatgacggg caacaatcat
    19381 tatcgtgacc gtttgaaaaa atcggtcttt ctcagtggtg atcccaagca gcctgtagat
    19441 cgtatggaac atgctttaca gcttattatt gaagcggatg gattaagcaa aaaagtgagt
    19501 gatttgaagc gctataagtg gagtaagttg aaagagaagt taaaagaaaa agtagagtct
    19561 ggccagttgt cacaacagga aatggaagag ttgcttgctg ttgaagacgc acgttgggat
    19621 gccattttag tggatgaatt taccttcgac tccatgaaga agaagacatt gcattccgtg
    19681 atagaaaaaa tgaaatctcc cttggcgtga tttttacaga cttttaacat cgatttacca
    19741 accagctatc aatccaaatg aggctggttt gctataatta aactatattt cctggaccaa
    19801 atatacattg ttgagagtaa gttatgccaa aatacacact gcccacaaga gatgccttat
    19861 taaaagcgat gcaagtaggt gaaacatcca ttgaagccgc tgaatacatg gcaacaaggt
    19921 ttgagcagat tttaacgaaa gcgaaattat taccagaatg taatgatatg ctggaaaaaa
    19981 taaaagaata tgctcaattc gttaaattca aattattatc ctctgctcaa gtttggtctg
    20041 gccaagaaag gccaacttca gattaccaaa acacgcagga aaacaaagcc gaatttttgg
    20101 caagccattt ggaagggtta ccaagtggat taaaactcga agttgctatt ggagacgatg
    20161 ccaaaatatt gcgagggttt tcttctaatg gaaaaatggt agaaggagat cagttgaaaa
    20221 ccatggatgg attattagaa ggatggcttg ccaagaatag tcttgctatt tcaggaggtg
    20281 cggttgttaa aatagacaat acgggcaacc aaaccaaagt agatccacag gaaatcagac
    20341 aattaatcaa cgatagcgaa aagggagtcg ccaaatattt tgcagataaa ggcgtgggca
    20401 tggaagtggc gcaacgaacc taccaagaac ccaaagcatt ggaaaccaag cgagaagaaa
    20461 tcagacaaga aattgaatcc ggtgctgaag ctccaaccac tcaatccatc agataggaat
    20521 atactggttc acctaaaaac caatcttggt ttttaggtga actgatttgc tgcctgcagt
    20581 ctatcgaaag agcctatgtc ataccaaatt cctgagtgaa cacttccagt ggcactattt
    20641 tgttccacat attttctgag taaaggggta acagaatacc ttccttgtct gcagttggca
    20701 aaaacctttg ggttatagca ggcaatacct gaaaaagtgt aagcctgatt tgtattggtt
    20761 aagtgggatc cattgattaa accaaaatcg ccatgatgaa gcaaagaagg atttttggga
    20821 attaaaagca gatgaaatgt atcaatattt ttgagttgga gcttggcaaa atcaaaatca
    20881 gtataaatgt cggcatttac tgttaaaaat ggctcttcgc ccagcaaagg caaggcgttc
    20941 acaatccctc cccctgtttc aaggcctcct ggcggttctg gagaatagat aacatttaag
    21001 ccccattgtt ttccatttcc tatatattgg cgaatttgtc cgcccaggta ggcatgattg
    21061 attatcaatc gttcaaagcc ggcattggcg aggttaatga tatggtgttc aattaagggt
    21121 ttgtttctta ccgtacaaag tgctttaggc attttgtcgg ttaaagggcg taatcgttct
    21181 ccacgtcctg ctgcaagaat catagcggtt ttcatggtaa ataaactcgt ttttgcagaa
    21241 aattaaaaaa tgggtgtaat tcttcatatg tttctgcgca ttctaatgca tattttaacg
    21301 tgagaggcaa gtccccaaga tatcctgctt tattatcacg caaatataaa cggcaaaaaa
    21361 cacctaacac ttttaaatgc ctttgtaacc cacatagatc aaaggcccgg ataaaatcgg
    21421 ttagagagta aatactggcg aggggggatt gttcatgaaa ataggttacc cattctaaaa
    21481 ctttttctct tggccatgaa atataacaat ctttgagtaa agaaaccaaa tcataggtaa
    21541 caggacctag catagcatcc tgaaaatcaa tagttgctaa acagctgtcc ttatttttga
    21601 ctagcatcag atttcttgaa tggtagtccc tgtgaatgaa aactcttggt tgagtcgcta
    21661 cttcacttgc gatccactcg atggtatgct gaaaaagttg taattcgtct tgttttaaat
    21721 caagagaaag gtaagctttc aaaaaccatt ccaaacataa attcatttct ttaagcatga
    21781 aacttttgtc aaataaggga agttcaggat cgtcagtggg acatttttga atttgtaata
    21841 gcaaattcat tgcctggtta taataattat ccactgtttc atgattcagc ttatttaata
    21901 ataattgatc ccctaaatcg cttagtaaca ggaaaccttc tcttttattt attgccagaa
    21961 tatcggggac tggtatttta attttatcaa gaacgttagc gatatggagg aaaggttcaa
    22021 tattttcctt ttcaggtggg gcgtccataa ctacttgagt taatccatta tattgaatgc
    22081 gaaaatacct tctaaaactc gcatctcctg ctaaaggaag gaggacaaag tctttttgtt
    22141 gaatcgtttg agctaaccac tctttcagtg cgttttctct ttcatgcatg gtatacttcc
    22201 cgtttttatt tacagagtgc ggttgatata ctcccatctt aataaagagt aatttcattc
    22261 gcgcacaaat aataaggtat tttgttagct gtgaacatga ggttttataa aaagctaatt
    22321 atatctggtt ttatttcatt tatcgcctta ttgcgaggtt tactctattg ccatttaaaa
    22381 agattattta aaaaatggcc tgaatcttta ttttcagtga aatggatctt aatggccata
    22441 gggataactg cttgtgtttt atctttgata aattatcaag gcttagccta ctctgctgcc
    22501 ttgataaatg aacctattca agcttgcgtg attgcgcgag aagtcgattt aacagacgat
    22561 ataagaacta aatttgccca atgtttgggt tggcaagctg atcaaagttc tcctgtgtgc
    22621 ctcggttttt ataaacccat tgcagtgaca ccgttagcta gtccagatga agtacgtatt
    22681 ctggcagata cggcttcctt ctatcgcact caaagatcca ctttatctgg gcatgtagaa
    22741 atgcagcagg gccaaagagt agttaacgct caaacagcct atgtgtacag ggatcctaaa
    22801 acgaatgaag taacaaaaat tgaatttttg aatcatgtac gttatttaga acctgataga
    22861 atgatgatcg ctagaaaagc agttgtgtat cctcaagata aatcgggtga agtggaagat
    22921 gttctttatc gatttaatac caatagaagc aatgcattgc tgcctgcctg gggtagggca
    22981 agtttaatca agcgttttgc aaatcaggac tactttttga aagaggcaac ttacacgact
    23041 tgcgcgcctc aagataaggc ttgggctatc gaggccgaat ccatcagtat agacaacgaa
    23101 aaaggaaagg gcattgctcg caatgccaaa ttacgtatcc acgaatggcc ggtgctttat
    23161 accccttatt taagttttcc taccaataga gatagaaaat cggggtttct gatgcccata
    23221 gtgggttatt ccaatgtagg gggggctgat ttgggcatcc cttattattg gaatatggcg
    23281 ccaaattatg acatgactct cgttcctcac ctttatacaa aacgcggttt gatgttggga
    23341 ggacaattta gatatttgac ctcaaagagc actggaacat ttaatggaaa ttttttacca
    23401 aaagataaag cctttggcag gtttttacag gacaatgagg tagagttccc tcagattcga
    23461 ggactttcca cgaatcgttg ggaagtaaat tttgtcgatt caacccaatt tctctctgat
    23521 cttcaattaa atgttaattt tcaacaggtt tctgatgatt attacttgca agatttcagt
    23581 acaaatttgg catcggtaac tcaaagacaa ttattgcgcc aggctgattt aacctacact
    23641 accgaaaact ggacttttag aggtatggga caaagttatc aaacgttaca tccaattaac
    23701 gaaatcccgg tttcccctgt ttatgaacgg ttaccgcaac ttatggcacg aggttattat
    23761 gatgatttac cttttaatgc ccaattcaac atccttggtc aatacgacca gtttcactgg
    23821 cctaatgact cctggaatat agcacttaat aatatgcctc aggggccccg gtttcatctg
    23881 aatcccattt tatcagtgcc aatgatgaag ccttggggat atgttactcc ctcagtgcaa
    23941 tttgtggaaa attattacga tatttcaaga aattatactt ggggcacatc tcgagctaac
    24001 tataacctga caatacctcg ctatagcctt gatggcggat tgtatttcga acgagatttg
    24061 catcttaagg gtacgtatta catacaaact ttagagccca ggttattcta tttaagagta
    24121 ccttattata accaaacgct tattccagtt tatgattcag gatttatgat ttttaatgtc
    24181 gatcaattgt ttagaaccaa tcgcttttca gggtttgata gaatcggaga tgccaatcag
    24241 ctaagttatg cccttaccac aaggtggtta gaagatgaat caggtgctga gaaagcgaat
    24301 ttttctattg gccaaatcaa atatttcagt gagagaagag tacaactttg tcaaagccct
    24361 accgggtttt gtaccgataa tccagatacc tttggcaact tatcgtctac ttttggaaca
    24421 tcgcctgttg cgtcaagggc ggtatataaa tttaatccag cttggggaat aacaggggat
    24481 tacatttggg atccggcaac aagggctaca aataatgcgg atttgaattt gcactatcag
    24541 ccagctcgta atgctatcat caatggcggg tatagttatt tggttaatgg tgacgttact
    24601 caagtaagaa ataatgacac tgaaaataat gccttacatc aagccatttt atccgctgcc
    24661 tggccactta gtgaaaaatg gagtggtatt ggagcctata gttataatat cagtaaaaat
    24721 tacagtatga tgtcatttct tggcgttcaa tatgatagtt gttgctgggc tatgcgtatt
    24781 ttgggagggc gaatttttag aagtttaaat gaagaatttg agcctcagta caataacaac
    24841 atttatttgc aaatattact aaaaggctta ggatcagtag ctagcagtga tccaagtggt
    24901 atattgaata cctatatccc aggatactat gaccctttcc gacgccgtta aaatccaaag
    24961 ataaatgaca caataaaagc gagtcaggtt taggtgttta ggttagtaca ggttgaaaat
    25021 attttaatct gttttttata aattatggtg taaattaagc aaacttgctt gtattttgac
    25081 aaagaataac ataaattgcc cgaaaaatgt gaatgaggat tatggcatgt ttaaaagaat
    25141 agcattggta tgcgctttgt tttcaggagt atgtttcgct gaaggaaagc agctgttaga
    25201 taaagtggtt gctatagtaa atgataatgt cattacttcc agcgaattga atgctcaagt
    25261 cgagttatct aaaaagcaga ttatagctca aaatatgcaa atgccagatg agtctgtatt
    25321 acgtaagcaa gtattgcaac atttaataga cgtagatctg gaaatgcaaa tggcaaaaca
    25381 aaatggtatt actattgaaa atgctgaaat tgatgaagcg atagaaaaaa tagctgcttc
    25441 aaatcatcta aatttatcgc aaatgcgcga tgaaattaca aagcaaggaa ttagctggca
    25501 agaatacaga caaaatatcc gtaaagaaat gctgatttcc agggttcagc aaaaagcagt
    25561 aggtaaagat atcattgtca ctaatgagca ggtggaacaa tacttaaaaa cttcaggccg
    25621 catagaaaat tctaacttga cgtatcattt aaaaaatatt gttatccctt taagcgaaga
    25681 accaacgacc aaacagcttc aaagagctaa aatcgaagca gaaaacttgt taaataaaat
    25741 taaaaaaggt gaagacttta gccgtttggc aatagaagaa tcaagtggag agtttgcact
    25801 ggaaggtggt gatttgggtg agcgtcattt ggctgagctg cctgaggtat ttgccaagga
    25861 agtagtacat atgaaagtag gtcaggtggc cggcccgata agagcaggaa atggatttca
    25921 tttgatcaaa ttagtagctg ttggcggcga gaatcagcgt catgtgatca ctcaaaccca
    25981 tgtcagacat atactgctta aacccgatgc cagcatggtg ccttctgaag caattaagca
    26041 agtcaataac atttaccggc aaatacagtc aggcaaagat tttgctctta tggctaaaca
    26101 atattcactc gatgctgcca gcgcagtgaa aggcggtgat ttaggatggg ttaatccagg
    26161 tgaattagtt ccagagtttg aaaaaaccat gaacagtttg ccattacata aagtcagtaa
    26221 acctgtaaaa acccagtacg ggtggcatct cattgaagtg attgcacgca gacagaagga
    26281 tgattcagaa gcttttaaga agcaacaggt caggcaattt ctacaacagc gcaagtttgt
    26341 agaggcagtg caaaactggc aacaacattt gcgttctcaa gcttatatta atatagttga
    26401 taaggattta gcatgaaccc actgcttatt agtagtggag agcctgctgg aattggcccg
    26461 gacttatgcc tggctttggc agaaaccgat ttgcccgtag tgatattggg tgatctgtct
    26521 ttattagagg caagagccag tgagttaaat ctcagtatta aatttttaga gtacagccct
    26581 catcaaagct tcaaaaaaaa agcaggatat ttaactgtat ggcctgttcc atgcgctgca
    26641 ccggtcatta gtggtgaatt aaatccgcaa aatgcggctt atgttatgga gcttttgact
    26701 ttaggggctt cgctatgctc caaaggggag ttttctgcat tagtgacagc gcctgttcat
    26761 aaggcaaaca ttaatgcagc cggtattacg tttactggcc acacggagtt ttttgctgat
    26821 ttctttgagg tggaaacagt ggtgatgatg ttggcctgtt ctcaaatgaa agtggcatta
    26881 gtcacaacgc atcttcctct gcgtatggtg cctgatgcca ttagctcttt gcttatcata
    26941 aaggtaattc aacaattaca tcattcatta aaacatgatt ttggaattca aagtccaaaa
    27001 atcaacgtgg ctggtctgaa tccgcatgct ggagaatcag gatatttagg tcgagaagaa
    27061 attgaaatta ttactccagc attaaatact ctaaaaaacc aggggatcga tgtgttaggg
    27121 ccgttaccag cagatacgat gtttataaca aaccatatca atcactgtga tgcttatgtg
    27181 gcaatgtacc atgatcaagg actgccagtc ttaaagtatg ctggatttaa tgaagcagta
    27241 aatataactc tcggattgcc gataattcgt acttctgtag atcatggaac agcattagaa
    27301 ttagctggta agaacaaagc taaccctggg agtatgcttg cggcggttaa gatggcgaag
    27361 gatatggctt taacaaggat aaaatgaaat gtctctgatt agcctgatag cagccataga
    27421 tgaaacagga ggattgggta ttaacaacca attactctgc tatttgccag ccgacttaca
    27481 gcactttaaa tcgattacca tgggtaagcc cattatcatg ggcagaaaaa cttatgaatc
    27541 gataggcaaa cctcttccag gcagactaaa tattgtgatt agtcaatcga ttcagtcaat
    27601 acctggtgtt gttgttgtcg attctttggg ccgggccata aaagagaccg ctgacgcatc
    27661 agaggtaatg atcattggag gagccaagat ttattctcaa gccatatcaa tggctaatcg
    27721 cttatatatt actcgaatac accatcaatt tacggctgat gtattttttc caaaaatcaa
    27781 tgaattggca tggtgctgcc gaagcaagga gtatcgacag catgatgaca aaaatcaata
    27841 cgatatgacg ttttgcgtat atgaaagaat atcaatttga tagctatcaa taatgataat
    27901 gtctgttttg tgagggatgt ctatgcactg gtggctgagt ccttacaatt atttcagttg
    27961 gtctgctcag tattctataa ttgtccgtta taatttcccg accaccataa gaaatattac
    28021 aatgtgagta accaggccat gttttccctg gctgaatact tccattgaag ttactttgac
    28081 agacaaataa aggattacca ttggtatcct tacccattaa caatgcatgc tctacgttgg
    28141 gttcccagct gtatcgccca aattcatttt gattcggtat ggtaaactgg ctaagaacgt
    28201 attctttacc accataaggt acgttgcatc gaccatatcc tgcccatgtt tttccgggct
    28261 gtatactgtt aaacagtttt gccctacaca gatacaatgc attgccattc gtgtcagttc
    28321 cagttcgtaa cgcattagct aacggtgaaa caggatgttg atggtaatga gtattggccc
    28381 aggcaggatt aagaaagaat atcatcaata aaaggtatac aagtttggac atatcaactc
    28441 tctaaagatt taaagagccg caagcataac gcaagaaaaa aatatgcgca agatgaatac
    28501 ttaaagatcg atatggttta tctattgtaa cccaaag
//
LOCUS       DACTJE010000023        27794 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.023, whole genome shotgun sequence.
ACCESSION   DACTJE010000023 DACTJE010000000
VERSION     DACTJE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 27794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27794
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.023"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            256..891
                     /locus_tag="I9760_000761"
     CDS             256..891
                     /locus_tag="I9760_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT5042548.1"
                     /translation="MDNTNHLFIFALIFTVLYHFLVQNLQYELLLKEQNYLLIADKEK
                     FHYLSHHDSLTNLPNRSYFHAHLQTLLENTDTKKEAITLYFMDLDKFKPINDEYGHEF
                     GDQLLLLTSKRLQSCFRKNDFIARLGGDEFTAIIVHKPHDQIAEALTLRIEQEFEQPF
                     VINGQTIHCTISVGIANYPRNAKNAEDLLKWADKAMYDNKRKKYQTLKQTS"
     gene            1025..1993
                     /locus_tag="I9760_000762"
     CDS             1025..1993
                     /locus_tag="I9760_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAT5042549.1"
                     /translation="MTIDRIRALVSEDFNAVNNLIIDKIQSQVGLIQDLSQHIVESGG
                     KRLRPLVVLLASNACGYKGKDHISLAAMVEFFHTATLLHDDVIDESTLRRGRQTANSI
                     WGSKASVLVGDYLFTQSVNLMVEVGIKEILNLLANTSHQITCGEVKQLANRHNPALTL
                     DDYFDVIRAKTALLFAAAACIGPILTNAPIELRNSLYNYGLHLGNAFQLIDDALDYCS
                     DAATLGKNIGDDLADGKATLPLIHALQNGNATQQQQIRESLMKGSLSHLPDILIAIEQ
                     TEAIPFTKRIAAQEVDSALSALEILPDSVYKEALVDLAKFALERNY"
     gene            complement(2020..2235)
                     /locus_tag="I9760_000763"
     CDS             complement(2020..2235)
                     /locus_tag="I9760_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042550.1"
                     /translation="MLLQIRDFIYREGVVSTQQLSREFHLDLTALQPMLDLWVRKGAI
                     RKCQGGANCKTACFKCRTPPDYYQSVV"
     gene            complement(2216..4471)
                     /gene="feoB"
                     /locus_tag="I9760_000764"
     CDS             complement(2216..4471)
                     /gene="feoB"
                     /locus_tag="I9760_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAT5042551.1"
                     /translation="MTHALLIGNPNCGKTTLFNALTNANQRVGNWPGVTVEKKTGEFL
                     LGEHLIEITDLPGVYSLVANAEGISQDEQIAAQSVIDLEYDCIINVIDACHLERHLYL
                     TSQLFELGKPVVVALNMMDIAEHRGISIDTEKLESLLGCSVIPIQAHKNIGIPALQQS
                     LLHCSQKIKPLKLSLSVAAQQILNDLENQLISKGYKNSFAYYFSRRLAEGDTLIGEKA
                     FTESLLIKLQETEQNLDVLLADARYQKIHEIVTLVQKKHSDASEHFTAKLDKLVLHRF
                     LALPIFFAMMYLMFLFAINIGGAFQDFFDISTETIFVQGSGWLLQQLHAPHWVIALVA
                     NGVGKGINTTITFIPVIAAMFFFLSLLETSGYMARAAFVVDKAMRAMGLPGKSFVPMI
                     VGFGCNVPAIMAARTLDSERDRLLTVMMSPFMSCSARLAIYAVFVAAFFPSGGHNVVF
                     SLYLIGILMAVFTGYILRKTTLKGHASPLILELPAYHRPSLRRLLRETSMRLRFFVYR
                     AGKLIIPICVILGGLNAITWGGGISSGEANTDSLLSIMGQWITPLFAPMGIHQDNWPA
                     TVGLLTGMLAKEVVVGTLNSLYAQVGHVGEIAAAHFDFWGGIKAAFGSIPANLSELGS
                     ALWNPVSASAADSELSQSVYGIMSRRFDGAVGAYAYLLFVLLYIPCVSTMAVIRQEAN
                     KRFMWTSIVWSFVVAYATSVVFYQGAKFLDHPQQSMVWILAMSLSLLFVLAVFRYSQY
                     GMGRQNAAANT"
     gene            complement(4468..4695)
                     /locus_tag="I9760_000765"
     CDS             complement(4468..4695)
                     /locus_tag="I9760_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAT5042552.1"
                     /translation="MQISELKQGDKVRLVSFGATDMQYRRRLLSLGITCGVEFSVVRM
                     APLGCPVQIEVRGTFLTLRKDEASQLVLEHL"
     gene            complement(4784..5866)
                     /locus_tag="I9760_000766"
     CDS             complement(4784..5866)
                     /locus_tag="I9760_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAT5042553.1"
                     /translation="MSLIEQYEAAIYRGEIDSDPEQREILEHMQRLAEDLQKKSDSWF
                     PWRKKHPIKGLYIYGPVGVGKTYLVDLFYQHIDEEKKARFHFHHFMQQIDAQLRRLQG
                     KKNPLQYIAKEMAKSIRLLCFDEFLVHDVAYAMILAELLQALHHYGVVLVVSSNTRPD
                     ELYLNGVHRERFIPAIEIIKKNCEVLNLNQKRDYRLGRQPLLEAYLYPLNEQTQEAME
                     KQFALITREEDCKGFITVQNRDIPYIKYGTRSIWFAFDVICNFPRSQLDYLEIADRFD
                     NVFLSDVYQLTESHTAQAIMFIHFIDVMYDRGIKLIISADVPAEELYVKGEMKETFKR
                     TLSRLIEMQSVDYLSRHPRRLVKEML"
     gene            complement(5863..6525)
                     /locus_tag="I9760_000767"
     CDS             complement(5863..6525)
                     /locus_tag="I9760_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042554.1"
                     /translation="MLFTDKLNTPGEHELMLEGLVGPLETVLTVPEDADTRYVAFLGH
                     PHSLQGGTMNNKVVTTMARVFKELHIPSVRFNFRGVGQSGGHYDNGIGESEDMRKLVR
                     QWHSEQPQAQNIFAGFSFGSYVAYRTASHCEHALLITIAPPVHHYDYKEFEPPPHPWL
                     IIQGKEDEVVPFELVSEFASQSSQVLPVIEFVETGHFFHGKLLILKEKLMDAVRDQVC
                     LS"
     gene            6762..7550
                     /gene="panB"
                     /locus_tag="I9760_000768"
     CDS             6762..7550
                     /gene="panB"
                     /locus_tag="I9760_000768"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAT5042555.1"
                     /translation="MKIHDFKIKKQEQKKISMLTCYDYPSACIVAESNIDCVLVGDSV
                     AMAVHGHPTTIMATIEMMELHTQAVARGLGKQFLITDLPFLGHKSSQGHTVENVKRLL
                     QAGAQAVKIEGADKDTCQTISHLVNAGIPVMGHIGLTPQSIHQLGGYKVQGKNSEQAE
                     TLLQQAATLEQAGCFAVVIECVPQELAKTITDSLVIPTIGIGAGPGTDGQVLVWHDML
                     GLQTSFNPKFVKKYFRAKDHFIEALNSYVQQVQQMHFPANEHSF"
     gene            7562..8320
                     /gene="panC"
                     /locus_tag="I9760_000769"
     CDS             7562..8320
                     /gene="panC"
                     /locus_tag="I9760_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAT5042556.1"
                     /translation="MQIFHNLNEWIHFRNTLSPDLSLGFAPTMGNLHAGHASLFLASS
                     KENHYTVSSLFVNPTQFNNPDDYKHYPRTVDADLELMTQNGVDFCILPNENEIYTDGY
                     AYQVQENRLGQLMEGKHRPGHFNGVLTIVMKLFNLVKPTRAYFGEKDYQQLLLIQGMV
                     KALFMDIEIKSCPTVREKSGLACSSRNNRLTPSQREIADEFAKIFHQNKSSAMISKEL
                     EALGITVEYIEEFQGRRFAAVKIGDIRLIDNYLL"
     gene            8317..8850
                     /locus_tag="I9760_000770"
     CDS             8317..8850
                     /locus_tag="I9760_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT5042557.1"
                     /translation="MIQTNWCVITGGPSSGKTTLINHLAELGYKTAPEIARFYINQLL
                     SHDKKTLQEIKHDILWLQRKILNIMLRRERNLPKKDLIFFDRGTPDSIGYFRFHHLDE
                     THVVRACRYLRYKKIFFCHPLPVVPDTIRDEDDVTAKKISDCIYNAYVGLNYPVIELP
                     ATSIKERLEIILSHVDT"
     gene            complement(8853..10268)
                     /locus_tag="I9760_000771"
     CDS             complement(8853..10268)
                     /locus_tag="I9760_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAT5042558.1"
                     /translation="MFKKILVANRGEIALRIVRACKEMGIAAVTIHSEVDRFALHVKR
                     ASHSHCLSKKPLDAYLNGRRIIECAKVAGCEAIHPGYGFLSENAEFAEDCNKAGIVFI
                     GPSAPVIATMGSKIAAREAMIKAGVPTIPGSKGNLETIDDALHCAETLGYPVMLKATF
                     GGGGRGIRLCHDASQIKQQFARVQSEAEKSFGDSHIYMEKYIANPRHIEVQILADHHG
                     TVLHLFERDCSIQRRHQKLVEIAPSPQLDEATRQVLLGYAVKAAKEVGYTNAGTVEFI
                     LDKNNRPYFMEMNTRLQVEHPITEQITGIDLVQEQIRIAAGERLTISQEQIKYNGFAI
                     EFRINAEDPQNDFLPSFGRITRYYAPGGPGVRTDSAIYTGYQIPPDYDSLCAKLTVWA
                     FDWPSVLRRARRALEEMRMFGVKTTIPYYLEMIKSEEFQKGVFNTGLVEAHPEWLKYS
                     NKSLPQHKAAVIAAAIALHKT"
     gene            complement(10261..10977)
                     /locus_tag="I9760_000772"
     CDS             complement(10261..10977)
                     /locus_tag="I9760_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAT5042559.1"
                     /translation="MYSSNYIYQHDDLELHGFLAHEEGIKQSRPAVIVAHDWSGRGEF
                     ACLKAKQLAEMGYIGFALDMYGQGQIGSNNDEKMALMQPLYSDRSLLRGRLYAALEAI
                     KSISVVDNNRIAIIGFCFGGLCALDLARSGADIRGAVSFHGLLNKPENLASGRIKAKI
                     LALHGYDDPMAKPEQVHAFCQEMTEANVDWQMHMYGQVKHAFTNPQAHDEHLGLIYDE
                     VAARRSWQAMSQFLQEIFNV"
     gene            11259..12125
                     /locus_tag="I9760_000773"
     CDS             11259..12125
                     /locus_tag="I9760_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5042560.1"
                     /translation="MMTDSLIHIRDLLHAQFCYQMFITPIHLPIDKKYREFAKMACEF
                     IEGRRDEIIHREYPRHHVLHRFTPASKPNGKKILITHGWMSRAAYMVRLIRLLHKEGY
                     EVYAIDFPAHGEAKGIQLPWTDAIAIIKETINQFGPFYGLVGHSFGGSMILNTLNLAG
                     QLPEWQLNHKPERAILIASPTQMRTPVNKIARRFKLSGQAYLQLRQLIRQQASVDPER
                     IRLSHFISQAPDTYFLCIHGELDATINPKESINFCKYYKNARLSLLPDADHVSVLMDE
                     RVEQIALDFLNR"
     gene            complement(12259..13113)
                     /gene="rpoH"
                     /locus_tag="I9760_000774"
     CDS             complement(12259..13113)
                     /gene="rpoH"
                     /locus_tag="I9760_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAT5042561.1"
                     /translation="MSQQLQLAAMNLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHS
                     EGDIEAARQLVLAHLRYVVRVARGYLGYGLPLSDLIQEGNVGLMKAVKRFDPKMGVRL
                     VSFAVHWIKAEIHEFVLRNWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAK
                     DLGVSREDVLLMEQRLNVMDSSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDT
                     GDQGREKLLFAMEQLDERSQDILQQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMK
                     KIRQYMEG"
     gene            complement(13410..14339)
                     /locus_tag="I9760_000775"
     CDS             complement(13410..14339)
                     /locus_tag="I9760_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAT5042562.1"
                     /translation="MLKRSQSLLAYHLQAAANSLNLFCRKPIATMMTVIVIAIALALP
                     TLFWVFTDNLSELTNRWQRGGHISLFLKPSLPEAEQTSLLEKVRTTEGVGQANIKSAA
                     DGLSELTQQEGMQDIMRYLPENPLPAVIEVVPALVIDSPAKLDLLSRKLQAFPQVALA
                     KLDMEWINRLHAILGFSGKAANALMALLALAVVFIIGNTLRLDIHNRQEEIKILKLIG
                     ATDPYIIRPFLYSGIWYGAAGALLAIFLVNIFILTLGVAVNQLANVYQMHYPLACLSL
                     RQILLLVLFAIILGWLGALLSVKRQLASIEPYK"
     gene            complement(14333..14983)
                     /gene="ftsE"
                     /locus_tag="I9760_000776"
     CDS             complement(14333..14983)
                     /gene="ftsE"
                     /locus_tag="I9760_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAT5042563.1"
                     /translation="MITFDQVSKRYPGGFEALSQVNFSLQKGEMVFLTGHSGAGKSTL
                     LKLIALLEWPTSGQLTVNGLKLNHLKKRDVAAHRSQLGITFQSPQLLNDRTVFDNVAL
                     PLQIQGVAYPMIAKRVHAALDMVGLLSKEKMLPVHLSVGEQQRVGIARAVVHKPALLL
                     ADEPTGNLDPKLSSEIMKIFEQFNQVGVSILIATHDLALIAGMKHRIVMLKGGRLC"
     gene            complement(14970..16037)
                     /gene="ftsY"
                     /locus_tag="I9760_000777"
     CDS             complement(14970..16037)
                     /gene="ftsY"
                     /locus_tag="I9760_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAT5042564.1"
                     /translation="MIKWFKRNQNSEPNLDAEEQQDHAKSSMESRLEEPQELTVKEPS
                     PKESIFARFRKGLSKTRHQLGEGISRLLLGKKEISQELLEELETLLISADLGLDTTQD
                     VLKQLNEGLARKQLSNGEAVYLTLKTNLQTILSQEEKPLSIETEDGSPFVILMVGVNG
                     AGKTTTIGKLAKQFQKQGKKVMLAAGDTFRAAAVEQLHVWGERNGIPVIAQHTGADSA
                     SVIFDALQAAKARKIDVLIADTAGRLHTQSNLMEELKKVKRVMQKLDPKAPHETMLVL
                     DASIGQNALAQAKQFHEAVQLTGITMTKLDGTAKGGILFAIANDLGIPFRYLGVGEGI
                     EDLRPFDAAQFVSAIFDDDHI"
     gene            16100..17425
                     /locus_tag="I9760_000778"
     CDS             16100..17425
                     /locus_tag="I9760_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAT5042565.1"
                     /translation="MHKLLISLMMLLSFQAFSQVQEYTLNNGLKVLVKEDHRAPVVVS
                     MIWYNVGSADEPVGITGVSHAIEHMMFKGTSKYPVGVFSKTIAALGGQENAFTNNDYT
                     AYYEKLDAGHLATSFELEADRMNNLLLNSEEFAKEIKVIQEERRLRTDNNPQALAFER
                     FLATAHLTAPYNHPVIGWMNDLKQMKVEDLKKWYESYYAPNNATLVVVGDVNPEKVHA
                     LAEHYFGSIAKRPIASRKPQQEPSALGKKMVYINAPAKLPLLLIGYTVPSVKTAKNNW
                     EPYALEIIAGILDAGESARFAKHLVRGNQVATGAEAYYNLYSRYQSQFIVYGAPSQDH
                     QIKDLEKALITELEALKKAPVSNQELQRVKNQIIAQKTFEKDSIFGQAMELGLLETIG
                     LGWKNTETYTKAINEITPEQIQQVAQRYFQENNMTVAELKPIRQEEVRP"
     gene            17422..18726
                     /locus_tag="I9760_000779"
     CDS             17422..18726
                     /locus_tag="I9760_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAT5042566.1"
                     /translation="MSVLRAFIISFSVIFSHNLFANSFKTEKWQTKNGVRVVFYQAME
                     VPMLDISLAFAAGSAYDGKYFGLSALTTNLINQGNSGKDATNIAEALADTGAQFNAET
                     SRDMVVLSLRTLTSKEALQQSTKTFSQIISHPDFPKEAFAREKDQLLMAVEQTEESPD
                     DVAIQNFFKTLYQEHPYAHPVHGTVESLNAIKENQVIDFYKKYFVAKNGILVMVGAID
                     SSQAHQLAEQLTQDLPAGEPAPTIPKASQLADAEKINVPFPSSQTVVRLGQIGIDHHN
                     QNYFPLMVGNYILGGGTLVSRLGTEVREKRGLTYGIDSQFVPMLGEGPFIISLSTKNS
                     EARNALHITQDTLIKFIKNGPNQEELASAKQYLTGSFPLSLGSNTNIANLLLRMAFYH
                     LPDNYLDTYVAKINAVTDAEIKQAFQQQVNPEKLLLVTVGQS"
     gene            18723..19268
                     /gene="rsmD"
                     /locus_tag="I9760_000780"
     CDS             18723..19268
                     /gene="rsmD"
                     /locus_tag="I9760_000780"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAT5042567.1"
                     /translation="MKQVIRIIGGLYRGKKIHFPDVDGLRPTPDRVRETLFNWLMNDI
                     KNARCLDAFAGSGALGFEAYSRGASHVVFIEQSPKAHASLQNTIRQFDTPKLHLIKTD
                     TLNYLKQSTEQFDLIFLDPPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKTE
                     LWKQLKLKQAGQVIYGLFEKL"
     gene            19343..19834
                     /gene="dotD"
                     /locus_tag="I9760_000781"
     CDS             19343..19834
                     /gene="dotD"
                     /locus_tag="I9760_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system lipoprotein DotD"
                     /protein_id="HAT5042568.1"
                     /translation="MNNNKIVIMFIFSALLAGCAGTMKFKKPPINNPSDDATIKLAEA
                     AVSVSDSMLEMAKVEKVITPPSKDNTLTIPNAYNLQARASVDWSGPIEELTARIAKAA
                     HFRFRVLGKSPSVPVLISISTKDESLAEILRDIDYQAGKKASIHVYPNSQVVELRYAK
                     IYS"
     gene            19815..20726
                     /locus_tag="I9760_000782"
     CDS             19815..20726
                     /locus_tag="I9760_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DotC family protein"
                     /protein_id="HAT5042569.1"
                     /translation="MRKFILSLSILLSALLVACSSRNHYGDTGSLAGLQAMADSKYTR
                     AQKKQKMGKIREMALKETALSVGAQAGLAWRAKIIDEQLNKQARNLDAIYDFNSLVLE
                     HNILPPVLLEGRNTLNLADAQSIRISDRTYKVAKQAHFITTPPTWRQYLWMDYVKPEA
                     PNVTLLPKTKAEKEIWCIYTERGWKNGIDQANTILEENIARIKEDFGGMILYRKLLAM
                     NMVSPPYVSHTDLGVTGDGSEIHIDDRVLRITALPELNVNSAEWRAAVAKDENALERF
                     KNMEKLANQAKIVITNKSWQPIIAPVS"
     gene            20750..21859
                     /gene="dotB"
                     /locus_tag="I9760_000783"
     CDS             20750..21859
                     /gene="dotB"
                     /locus_tag="I9760_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system ATPase DotB"
                     /protein_id="HAT5042570.1"
                     /translation="MPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRL
                     LKITNRRLSNTELGDLINSIYGPNATTQLLSGKDIDTHYEFRPNRGVRYRYRVNATAC
                     LVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGSGKSTLLAS
                     IIRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRR
                     KPRLIMVGECRDAETISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRT
                     IDILETIRLCIWQKLVPTVDERRVALREYLVFDEEVRDILLEGDPNEVTSATRKLVRQ
                     KGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ"
     gene            21942..23669
                     /locus_tag="I9760_000784"
     CDS             21942..23669
                     /locus_tag="I9760_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAT5042571.1"
                     /translation="MMFHRKSLYHYFNLFILLILGSSLNWASVNQHSTASPTLNIKIL
                     GINDFHGQISSGRAVKNEPVGGAAVLAAYLKEAQLGMEDRSIITIMGDLVGASPPSSG
                     LLNDEPSILFINGLGNNQCKPESRMDPLCNIVATVGNHEFDKGQKAMFDLIYGTNNPP
                     TDSWISLPNYPGASYPYVSANIVDAKSEQPLFPPYIIKRIHNTPIAFIGAVLKDAADS
                     MFPANAEGVKFLDEADSINHYIPEIKAKGVNVIIVLIHEGGNQIPYEGDTQTTSAVEG
                     HIKEIVYSLGDEIDVVMGGHTHQFLNAFLPNRNGHKILVTQANSYSAAFAEVTLQIDT
                     KNHTVKNKSAKIITTYANRWPGTNPDTQALEIVKLAEDKIGPMINNHVGTAEYDLLRK
                     QNKDGESSLGNLIADAYRTVMNTDLGITNPNGIRDDLKAGEVNWGNIYSILPFSNRIV
                     TITLTGNDIYDLLEQQWMGSYINMLQISGFAYTYDSHQPLGHKIIEIRHQNKPLLREK
                     MYTIATSDFLASGNGVFSVMKRGKIIHIGQSDHDTVIAYIKSLPQPFHASIEARIKES
                     ETGLKTGVG"
     gene            complement(23711..24508)
                     /locus_tag="I9760_000785"
     CDS             complement(23711..24508)
                     /locus_tag="I9760_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5042572.1"
                     /translation="MPSTYILNTNDKARERLSLQHQLYAKSSLDLLSEVGPSAAMTAL
                     EIGCGSGDMTLELAKLIGREGSLLTIDLSQDQLDHTQQRTRTYSNIRFKVWDVNHLSD
                     LGEKFDLIYCRMVLHHVADARSVILQMKNCLNSGGFVVCEEPSLFDSTFCSPPSPSYD
                     QFTQWARSCFSKSNRDFQIAHRLEQEFSSCGFEIEHCSLFQPLLRTNKEKLIYSMALD
                     DLTPQLLELGIAKREEIKQLHNELEDLAKTNSTMCWIRMHKIIAKLN"
     gene            complement(24538..25482)
                     /locus_tag="I9760_000786"
     CDS             complement(24538..25482)
                     /locus_tag="I9760_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAT5042573.1"
                     /translation="MKPFVYLTNQFLEPIIPMLIPDWNIIHGWKMASRVDQSRVVALA
                     TTVWDQIDHSFLMQFPNLKIISHLGIGTDNIDINFLKQNHIILHSQPNAGVHDTAELA
                     IALLLTLSRRVILNDRYTRNNEWVEKKPRFLGNHLLGKQLGLVGFGQIGEKIAQFAEP
                     FGLKIAYTARSQKNSPYLYCPTAANLASISDFLIICCSGGIDTQHLINKQVLDNLGPE
                     GYLINVARGSIVDQNALIDALQRHTIAGAALDVYQYEPEVPFALRQLDNVVLSPHMGS
                     STKENLNQMFQLQAKHLNQYLNESLWQRESHVTLFEEQ"
     gene            25719..26741
                     /locus_tag="I9760_000787"
     CDS             25719..26741
                     /locus_tag="I9760_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl peptide synthase"
                     /protein_id="HAT5042574.1"
                     /translation="MPVIPVEEFDGFILKLGKNNYHFCKNETPFNNSCSANIAIDKYC
                     TSKLLEKAGIPVPKAISLNCSEFQRNLHKDKIAQLNFPLVIKPIDGSLGIGVLCNIKT
                     WEELESHLTKYFSSYQCLIIEEFHGKLNSYRVLVFNNRILGIVQRYPAAVTGDRVHPI
                     HELIKQENLNRKRINEALGEITLDEEARIKLQELGITENYIPSFGEQIVLCYTSNATR
                     GGSYVTINKKMCKQNRKLLLQAAKILDLQLVGIDVECSDIINIPIEQSNGVILEVNHK
                     PSIRIHEFPMKGTPQKVTKKIMRSFITRHPLSYLYSIYNNQPTAFYVRSSILIIIMGL
                     ILLAVW"
     gene            27016..>27794
                     /locus_tag="I9760_000788"
     CDS             27016..>27794
                     /locus_tag="I9760_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HAT5042575.1"
                     /translation="MNPVSILDASLRDGGHRTNFHFKDDDLKKILLPLDNSGIEYIEI
                     GYRNGSLHPIENIGKAGMCPQDYLLYCQSLIKKAKIAVMVHPHNVNEQDLFELKNCGV
                     KLIRICIAKGDLVNAVPLIKLAKNLNFETSVNIIHISYYTESELDSLIEEVSEHEPDM
                     IYFADSNGSLFPDRINCLYKKYTRQYKVSFGFHAHDNLGLAQANALAAVNAGVHFIDA
                     SLAGMGKGTGNLKTEFFIAYLHANNIKKYNLEDVLTAANYV"
BASE COUNT         8759 a         5842 c         5172 g         8021 t
ORIGIN      
        1 aacttcaatc tggccttgag tgggcacatt ttgaatattc ttgtgttgtc tatcatcatt
       61 ggaggcaatc tcatcgttgg aaaaactacc atgtcttacc tgggatggtt ctatgtttct
      121 ccaatcgttg cttttgctac acttggatta accgggttag tcgtctatgg cctgctggcg
      181 attggggcaa tgtttctttt tatattcgaa ttctacatcc ccatctattc catttctcag
      241 gaatatgtat tttttctgga taataccaac cacctgttta tttttgccct tattttcact
      301 gttctttatc attttctggt tcaaaactta caatatgaat tgctgttaaa agagcaaaat
      361 tacctgctca ttgctgataa agaaaagttc cattatcttt cgcatcacga ctcactaacc
      421 aacttaccca accgctctta ctttcatgct catcttcaga ccctattaga gaacactgac
      481 actaaaaagg aagccattac tctttatttt atggatttgg ataaattcaa acccatcaat
      541 gacgaatatg gtcatgagtt cggtgatcag cttttacttt taaccagcaa gcgtttgcaa
      601 tcctgcttta ggaaaaatga ttttatagcc cgactgggag gagatgaatt taccgcgatt
      661 atcgttcata aaccccatga tcaaattgct gaggctctaa ctcttagaat tgaacaggaa
      721 tttgaacaac cttttgtcat aaatggccag accattcatt gcaccataag cgttgggata
      781 gccaattacc cgcgcaatgc aaaaaatgcg gaagatttgt taaaatgggc tgataaagcg
      841 atgtatgaca ataaaaggaa aaaatatcaa acgcttaaac aaacctctta accaacattc
      901 ctaatttaat cagtatgatt gggccttcat tttatcaagc acaggatgca gcaatctgac
      961 attcacttga caattaactt ttacaacttt agcattcatt gattaaatat cagagggtaa
     1021 atgaatgact attgaccgta tacgagcgct ggttagtgaa gattttaacg cggttaataa
     1081 tttaattatt gataaaattc aatctcaagt tggtttaatt caggacttgt cacaacatat
     1141 agtcgaaagt ggcggcaaac gtttacggcc tttggttgtc ctgttggcaa gcaatgcttg
     1201 tggatacaaa ggaaaagacc atatttctct tgccgctatg gttgagtttt tccatactgc
     1261 cactttgttg catgatgatg tgattgatga atccacttta agaagaggtc gacaaactgc
     1321 aaatagcatt tggggcagta aagccagtgt tttagttggt gattatcttt ttacccaatc
     1381 cgtaaatcta atggtagagg ttggcattaa agaaattctc aatctactgg ccaatacttc
     1441 ccaccaaatt acctgtggcg aagtcaaaca acttgccaat cgccacaatc ccgctttaac
     1501 ccttgatgac tatttcgacg tcatccgggc caaaacagct cttttatttg ctgcggctgc
     1561 ctgcataggt cctatactca cgaatgcgcc tattgagctt cgaaatagtt tatataatta
     1621 cggtttacac ttgggaaatg cctttcaatt gattgatgat gccttggatt attgctcgga
     1681 tgctgccact ttaggtaaaa acattggcga cgatttggct gatggaaaag cgactttacc
     1741 actaatacat gcgttacaaa atggtaatgc gactcaacaa cagcaaataa gagaaagttt
     1801 aatgaaaggc agtctaagcc atctgcctga tattttaatt gctattgagc aaaccgaagc
     1861 cataccgttt acgaaacgaa ttgctgccca agaagtagac tcagcactat ctgccctgga
     1921 aatactgcct gactcagttt ataaagaagc tttagtcgat ttggccaaat ttgccttgga
     1981 aagaaattat taacctgata attgcatcga ttctaataat tacacaaccg attgataata
     2041 atctggcgga gtgcggcatt taaagcaggc agttttgcaa ttggctcctc cctgacactt
     2101 ccttattgcc cctttcctaa cccataagtc cagcataggt tgcaatgcgg tcagatccaa
     2161 atgaaactcc ctactcaact gttgagtact tactacccct tcccggtaga taaaatcacg
     2221 tatttgcagc agcattttgc ctccccatac catactggct ataacgaaat acagctaaaa
     2281 caaaaagcag gctcagtgac atagccaaaa tccagaccat actttgttga ggatgatcca
     2341 gaaatttagc gccttgataa aagacaacgg aagtggcgta agccacgaca aatgaccaaa
     2401 caatggaagt ccacataaat cgcttgtttg cctcctggcg aatcactgcc atagtcgaaa
     2461 cacaagggat ataaagcaaa acaaaaagca aataagcgta ggctcctaca gctccatcaa
     2521 aacgtcgtga cataatgcca tagacagatt gagataactc actatcagct gcactggcag
     2581 aaacagggtt ccacaatgca gagcctaatt cagacaaatt ggcgggaata gacccaaaag
     2641 cagctttgat gcccccccaa aaatcaaagt gagcagcagc tatttctccc acatggccta
     2701 cttgggcata aagtgagttt aaggtgccaa ccactacctc tttagccagc atgccagtta
     2761 ataaaccgac agtcgcgggc cagttgtctt gatgaattcc cattggagca aataatggag
     2821 taatccactg acccattatt gacaataaag aatccgtatt ggcttctccg gagctaatcc
     2881 ctcctcccca agtaatcgca tttaatcctc ccagaatcac acaaatggga ataatcagct
     2941 tacccgcacg ataaacaaaa aatcgtaatc gcatggaggt ttctctcagc aatcttctga
     3001 gtgagggtct atgataggcg ggtaactcca aaatcaaagg agaagcatgt cctttcaacg
     3061 tagttttgcg taaaatatat ccggtaaata cggccattaa aatgccaatc aaatacaacg
     3121 aaaaaacaac attatgcccg ccagagggga aaaaggccgc gacaaatact gcataaatag
     3181 ccagtcgggc actacaagac ataaatggac tcatcatcac agttaataat cgatcgcgct
     3241 ccgagtccag ggtgcgtgct gccataatgg cagggacatt acatccaaaa cccacaatca
     3301 tgggtacaaa tgattttcct ggtaatccca tggctcgcat cgctttatcc acgacaaaag
     3361 cagctcttgc catatagcca gaagtttcca ataaggacaa aaagaaaaac atcgcggcaa
     3421 tcacaggaat aaaagtaata gtggtattaa ttcctttgcc taccccatta gctacaagcg
     3481 cgattaccca atgtggtgca tggagctgtt gcaacaacca accactaccc tgaacaaaga
     3541 tagtctctgt actaatgtca aaaaaatcct ggaatgctcc cccaatatta atagcaaaca
     3601 aaaacatcaa atacatcatc gcaaaaaata tgggcaaagc taaaaagcga tgcaaaacaa
     3661 gtttatccaa tttagcagtg aagtgttcgc tggcatcact atgctttttt tgaaccaacg
     3721 taacaatttc atggattttt tgatagcgag cgtctgccag taacacatca agattttgct
     3781 ccgtttcttg aagctttata agaagactct cggtaaaagc tttctctcct atcaaagtat
     3841 ccccttcggc aagcctacgg gagaaatagt aggcaaagga atttttatac ccttttgaga
     3901 tcagctgatt ttccaggtca ttcaaaattt gttgtgctgc aaccgataag gaaagtttta
     3961 gaggttttat cttttgggag caatgaagta atgattgctg aagtgccgga ataccaatgt
     4021 ttttatgggc ctgaatggga ataacagaac accctaacaa actctcgagt ttctctgtat
     4081 caatagaaat accccgatgc tcggcaatat ccatcatgtt aagtgctaca acaacaggtt
     4141 tacctaactc aaaaagttgg ctggttaaat acaaatgtct ttcaaggtga caagcatcta
     4201 taacattaat gatgcaatcg tattccaaat caataactga ctgagcagca atttgctcat
     4261 cctggcttat tccttcggca ttcgcaacca gagaatagac acctggcaaa tcagtgattt
     4321 caattagatg ttctccaagc aaaaattcac ctgttttttt ctcaacagtt actccgggcc
     4381 agttgcctac gcgttgattc gcattggtca aggcattgaa tagggttgtc ttaccacaat
     4441 tgggatttcc tattaatagg gcatgagtca tagatgctcc aacactaatt gactcgcttc
     4501 atctttacgc aaagttaaaa atgttcccct tacttcaatt tgtaccggac accctaatgg
     4561 agccatcctc actacagaaa actcaactcc acaagtaata cccagggata aaagcctgcg
     4621 tcgatactgc atatcagtcg caccaaaact gaccaatcgt actttatccc cttgttttaa
     4681 ttcactaatc tgcataatat ataaacaata cggtgattca tttaattaat tttaacacaa
     4741 acgatatcga gaatcattct caattaagaa attttatacc acattacagc atttccttta
     4801 ccaaacgccg aggatgacga ctcagatagt ccaccgattg catctcaatt agacggctca
     4861 acgttctttt aaacgtttcc ttcatttcac ctttaacgta caattcttct gcaggaacat
     4921 ccgctgagat aatgagctta atgcccctat catacataac atctataaag tgaataaaca
     4981 taatcgcttg tgctgtgtga ctttcagtaa gctgataaac atcactcagg aagacattat
     5041 caaagcgatc agcaatttct aaataatcca attggctacg aggaaaatta caaatcacat
     5101 caaaagcaaa ccaaatagac cttgtcccgt atttaatata tggaatatca cgattttgta
     5161 ctgttataaa acctttgcag tcttcttccc gggttattag agcaaactgt ttttccattg
     5221 cttcttgtgt ttgttcattt aaaggatata aataagcctc taacaagggt tgtcgaccta
     5281 aacgataatc tcgtttttga ttaagattca gaacctcaca atttttttta ataatctcga
     5341 tagccggaat aaatctctct ctatgcaccc catttaaata caactcatcc ggacgagtat
     5401 tcgaagaaac aaccaaaaca acgccgtagt gatgcagtgc ttgtaataat tcagccaaaa
     5461 tcattgcata agccacatcg tgaactaaaa actcatcaaa acacagtaaa cgaatcgatt
     5521 tggccatttc tttggcgata tactgcaaag gatttttttt gccttgtaat cgtcttaatt
     5581 gagcgtcaat ttgctgcata aaatgatgaa aatgaaatct cgcctttttt tcctcatcaa
     5641 tatgctgata aaacaaatcc accaaataag tcttgccaac tcccacaggc ccatagatat
     5701 aaagcccttt aatgggatgc ttttttctcc agggaaacca agagtcactc ttcttctgta
     5761 aatcctcagc aagcctctgc atgtgttcaa ggatttctct ctgctcagga tcgctatcta
     5821 tttcacctct ataaatcgcg gcttcatact gctcaatcaa actcatgaca ggcaaacctg
     5881 atcacgaaca gcatccatca gtttttcttt taatattaaa agcttaccat gaaaaaaatg
     5941 acccgtttca acaaattcaa tgactggcaa tacctgcgat gattggctgg cgaactcact
     6001 caccagttca aatggcacaa cctcatcttc tttgccttgt ataataagcc agggatgagg
     6061 aggtggctca aattctttgt aatcataatg atgcacagga ggagctatcg ttattaacag
     6121 agcatgctca caatgagacg ccgttctata agccacataa gatccgaaag aaaagcccgc
     6181 aaaaatattt tgggcttgcg gttgctcaga atgccactgc ctcacgagct tgcgcatatc
     6241 ttcactctcg ccaataccat tatcataatg gcctccagac tgccctacac ctctgaaatt
     6301 aaaccgtact gaaggaatat gcaattcctt aaaaactctg gccatcgtgg taactacttt
     6361 gttattcatt gtcccacctt gtaaagaatg tggatgacca agaaaagcaa catagcgagt
     6421 atccgcatct tcaggaacag tcagtacggt ttccagtggg ccaactaatc cttcaagcat
     6481 taattcatgc tctccgggag tatttaattt gtcggtaaat agcataacaa ccctgccaat
     6541 ttaaagataa taacctaaat caatatggat ttaagcaaaa gagttaggct attatagcaa
     6601 tggatgcgtt gacccccaat agaccttttg tatatctggt ttattttgtt ggcagcgact
     6661 tttattgacc ttcagaggag gacttgatcc atgagacagg tacctgtatt accatcattc
     6721 ttatctattc caggcgcatt ctcctattca gggagaacgg catgaaaatc catgatttta
     6781 aaataaaaaa gcaggaacaa aagaaaattt ccatgttgac ctgttacgat tacccttcag
     6841 catgcatagt agcagaatca aatatcgatt gtgttctcgt gggtgattcc gtagctatgg
     6901 cagtgcacgg ccaccctaca accattatgg ccactatcga aatgatggaa ttacataccc
     6961 aggcagtagc tcgtggttta ggcaaacagt ttttgattac tgacttacct tttctcggac
     7021 ataaatcatc tcaaggtcat acagtagaaa acgtaaaaag gctgttacag gctggagccc
     7081 aagcagttaa aatcgagggc gctgataaag acacctgcca aacgatttcc catttagtta
     7141 atgccggcat ccctgttatg ggtcatatcg gcttaactcc ccaatccatt catcaattag
     7201 gcggttataa agtccaggga aaaaacagcg aacaggcaga aactctatta caacaggcag
     7261 ccaccctgga acaagccggc tgctttgcgg tagttattga atgtgttccg caggagctgg
     7321 caaaaacgat taccgattcg ttagttattc ctactatagg aattggcgca ggcccaggaa
     7381 ctgatgggca agtccttgtc tggcacgata tgctaggttt acagacttca tttaatccta
     7441 aatttgtaaa aaaatatttt agagccaaag accattttat tgaagcctta aacagctatg
     7501 tccagcaagt acaacaaatg catttcccag ctaatgaaca ttctttctag gaaaattatc
     7561 catgcaaatt tttcataatt taaatgagtg gattcacttt agaaacaccc tgtcccctga
     7621 tttatccctg gggtttgctc caactatggg taacttgcat gctggacatg cttctttgtt
     7681 tttagccagt agcaaagaaa atcactatac cgtctccagt ttgtttgtta acccgaccca
     7741 atttaataac cctgatgatt acaagcatta tccacgcaca gtcgatgctg atctcgaatt
     7801 aatgactcag aatggagttg atttttgtat actccccaat gaaaatgaaa tctataccga
     7861 tggctatgct tatcaagttc aagaaaaccg gcttgggcaa ttgatggagg gaaaacaccg
     7921 accggggcat tttaacggag tactgaccat cgtcatgaaa ctctttaatt tggtcaaacc
     7981 aacccgtgcc tattttggtg aaaaagatta ccaacaactg ttgctaattc aaggaatggt
     8041 aaaagcattg ttcatggata ttgaaatcaa atcttgcccc acagtaagag aaaaaagtgg
     8101 cctagcctgc agctcacgca ataacaggct tactccaagc caaagagaaa tcgctgatga
     8161 atttgcaaaa atatttcatc aaaacaaatc ctctgccatg ataagcaaag aattggaagc
     8221 gcttggaatt actgtagaat atattgaaga attccagggc agacgatttg ctgcagtaaa
     8281 aattggcgat atccgtctca ttgataatta tttgctatga ttcaaaccaa ttggtgcgtg
     8341 ataacaggtg gaccttcctc agggaaaacc actttaatta atcatttggc agagttggga
     8401 tacaaaactg cgccagaaat agccagattt tatatcaacc agctgctgtc tcatgataaa
     8461 aaaactctac aagaaataaa acatgatatc ctttggttac aaagaaaaat tctcaatatc
     8521 atgttaagac gagaaagaaa cctgcctaag aaagacttaa ttttttttga ccgtggcacc
     8581 ccggacagca tcggttattt tcgttttcat catttagatg agactcatgt ggtcagagct
     8641 tgtcggtatt tacgctataa aaaaattttt ttctgtcatc ccttgcctgt tgtcccagat
     8701 accataagag atgaggatga tgtcaccgca aagaaaatta gtgactgcat ttataatgcc
     8761 tatgttggcc ttaactaccc tgtcatagaa ttaccagcaa cctcaataaa agaacgcctg
     8821 gaaattattt tatcccatgt tgatacctag ctttaggttt tatgcaaagc gattgccgct
     8881 gcaataaccg ctgccttgtg ttgaggcaag gatttatttg aatactttaa ccattcgggg
     8941 tgtgcctcca ctaaaccggt attgaatacc cctttctgaa attcctctga ttttatcatt
     9001 tctaaataat aaggaatggt agtttttact ccaaacatgc gcatttcctc aagagctcga
     9061 cgcgcccgtc gaagtaccga gggccaatca aaagcccata cggtcaattt ggcacacaaa
     9121 gaatcataat ccgggggaat ctggtaacca gtataaattg cgctatctgt ccttacacca
     9181 ggtcctccgg gagcgtaata tcgagtaatc cttccaaaac tgggtaaaaa atcgttttga
     9241 gggtcttctg cattaatgcg aaattcgatg gcaaaaccat tgtatttaat ttgttcttga
     9301 gagatagtta gacgctcacc agctgcaatt cgaatttgct cctggactaa atcaatacca
     9361 gtaatctgtt ctgtaattgg atgttcaact tgtaaacgag tgttcatttc catgaagtaa
     9421 ggtcgattat ttttatccaa aataaattca acagtacccg cattggtata ccctacctcc
     9481 ttggctgcct tgactgcgta tcccaaaagc acttgacgtg tcgcctcatc caattggggg
     9541 gaaggcgcta tttcaaccaa tttctgatgt cgcctttgga ttgaacaatc ccgttcaaac
     9601 aaatgaagta ctgttccatg atgatctgct agaatttgta cttcgatatg gcgtggattc
     9661 gctatatatt tttccatgta gatatgggag tctccaaatg atttttctgc ttcagattgc
     9721 acgcgagcaa actgttgctt tatctgactg gcatcatgac ataatctgat acctctaccc
     9781 ccccctccaa aagtcgcttt gagcattaca ggatacccta gagtttctgc gcaatgtaaa
     9841 gcgtcatcta tagtttccaa attccccttg cttcctggta tggtgggaac ccctgctttt
     9901 atcatcgctt ctcttgctgc aatcttggag cccatagtgg ctatcactgg agcagacggt
     9961 cctataaaaa cgatacctgc cttattacaa tcctcagcaa actcggcatt ttcagataaa
    10021 aaaccatacc cggggtgaat tgcttcacaa ccggccacct tggcacactc aataatacgc
    10081 cgtccgttaa gataagcatc taaaggtttt ttactaagac agtgagaatg agaagctctt
    10141 ttgacatgca gggcaaatct gtccacttca ctgtgaatag tcactgctgc tatccccatc
    10201 tccttgcaag cacgtactat tcgcagggct atctcaccgc gattagccac cagaattttt
    10261 ttaaacatta aaaatctctt gtaaaaactg gctcattgct tgccatgatc ggcgcgcagc
    10321 aacctcatcg taaattaatc ctaaatgttc atcatgcgct tgaggattag taaaagcatg
    10381 tttcacctgc ccatacatat gcatttgcca atccacatta gcttctgtca tttcctgaca
    10441 aaaagcatgt acttgttccg gtttcgccat aggatcatca taaccgtgca aggccagaat
    10501 ttttgcttta attcttccgg atgctagatt ctcaggttta ttaagcaagc catgaaaact
    10561 tactgctcct cgaatatccg caccactcct ggctaaatca agggcacaca aaccaccaaa
    10621 gcaaaaccct ataatggcta tgcgattgtt atcgactacc gatattgatt taatggcttc
    10681 caatgcggca tacaaccgtc ccctaagcaa agagcgatcg ctgtacaagg gttgcatcaa
    10741 tgccattttt tcatcattgt tagaaccaat ttgtccttgc ccatacatat ccaaagcaaa
    10801 gcctatatag cccatctcag caagttgttt tgctttaagg caagcaaact cacctcttcc
    10861 actccaatca tgagcgacta ttacggcagg ccgagattgt ttaattccct cttcatgcgc
    10921 taaaaatccg tgtaactcaa gatcatcatg ttgataaata taattcgatg aatacataaa
    10981 atatctccta aattatttta aaatagtcta gggcaatatc cgtaaaagtc cagtaattac
    11041 attagtttag cctaatgctt ttctttgtct tttgttcctc gtggctaagt aaaaatagtg
    11101 tcgtccaata aaaggatatc gaattgaatt aatctaatgc tcttgcatgt ttcgccaaaa
    11161 catgagtcca tgagtcagta aatatcaata tgtccactaa ttaactcaaa ttatggattt
    11221 ggtctaaaat aaaaataaat tatgtgagga atgttgccat gatgactgat tcgttaatac
    11281 acataaggga tttgttacat gctcaatttt gctaccaaat gtttattact cccattcatc
    11341 ttcctatcga taaaaaatac agggaatttg ccaaaatggc ctgtgaattc atagaaggaa
    11401 gacgggatga aatcatacac agagaatatc ctcgtcatca tgttttacat cgttttactc
    11461 ccgcgagcaa accaaatgga aaaaaaatat tgattacgca tggctggatg tctcgtgctg
    11521 cttatatggt tcgcttaatc aggcttcttc ataaagaagg atatgaagtc tatgctatcg
    11581 attttcctgc ccatggcgaa gctaaaggaa ttcagcttcc ctggactgat gccattgcta
    11641 ttatcaaaga aacgattaac caatttgggc ctttttatgg attggttggc cattcttttg
    11701 gtggttcaat gattttaaat actttgaatc tggcaggcca actaccggaa tggcaattaa
    11761 atcacaagcc ggaaagagcc attttaattg catcgccaac acaaatgcga actcctgtca
    11821 ataaaattgc cagacgtttt aaattaagcg ggcaagccta tttacaactt cgccaattaa
    11881 tccgtcaaca agcatcggta gatcctgaga gaattcgttt aagtcatttt atttcccaag
    11941 cccctgatac ctattttcta tgtatccatg gagaattgga tgccactatc aaccccaaag
    12001 aatcgataaa tttttgcaaa tattacaaaa atgcccgcct gtctctttta ccagacgctg
    12061 atcatgtgag tgtattaatg gatgagcgcg tcgaacaaat cgctttggat tttttaaatc
    12121 gttaatatga cagggggggt tgatccacct ggattacggt tttacctaat ccaggtggat
    12181 ccagtagcta ggcagaccat tgtgtttgct gaaacaatga gtttcacata cagaggaatt
    12241 ggcttcaaaa ttttgtaatt aaccctccat gtattgacgg atttttttca tggcattttt
    12301 ttcaagttgc ctaacccgtt cagcagagac accatatttt tctgccaaat catgtaacgt
    12361 gagtttttct tcagcaagcc agcgttgttg taaaatatcc tggctacgtt catccagttg
    12421 ttccatagca aacagtaatt tttcacgccc ctgatcgcca gtatcctcat tctctaacaa
    12481 gacagcagga tcatcgttca cattaaacaa ataacgctca ggcgctttgt aagcatcatc
    12541 attatcatca acatctggcg catcataaga tgaatccata acatttaaac gttgctccat
    12601 caacaacacg tcttctcggc taacgcccaa atctttggcg acagcgtcaa cttcttcatt
    12661 gctgaaccaa cccaatcgat ttttcatttg gcgcaaatta aaaaataatt tacgttgggc
    12721 cttggttgta gcaactttaa ctattcgcca gttacgcagc acaaactcat gaatttctgc
    12781 tttaatccaa tgaacagcaa aagaaacaag gcgtacgccc atcttggggt caaaacgttt
    12841 tacggctttc atcaagccta cattaccttc ctgaattaaa tcacttaaag gcaatccata
    12901 ccccagataa ccgcgggcta cacgaactac atagcgcaaa tgcgcaagaa ctaattgtcg
    12961 agcagcttct atatctcctt cagaatgaaa acgctcggca tatgcaattt cctcttccaa
    13021 agtcagcatt ggaatttgat ttactcgatg gatataagaa tcaagactac caacaggtag
    13081 attcattgca gcaagttgca actgttgact catactactt cctccggtca ttaacactaa
    13141 aatagtatta aaatttatat cctattaact gataggattc accaaaatga atgctaagct
    13201 ataaattttc aaaaactgca aattaagtaa aagttcataa cttagcactc tcttcatgat
    13261 gtttcaattt tggtcattat ttcagcgatc cttggcatat ttataaatcg ctaaacctct
    13321 tatataaaag ttttttacgg tttataagca cacaacgaat ccggacaaat attgaccaaa
    13381 accaatgctt catcaactaa tattatacat tatttgtagg gttctataga agccaattgc
    13441 cgcttaacag acaacaaggc tccaagccac ccgagtataa tagcaaataa cacaagtagc
    13501 aagatttgcc gtagtgacaa acaagctaaa ggatagtgca tttgatagac attagccagc
    13561 tgattcactg cgacgcccag ggttagaata aaaatattaa ccagaaagat ggctaaaagt
    13621 gcgccagctg ccccatacca aattccagaa tataaaaaag gacgaataat ataaggatct
    13681 gttgcgccaa tcagttttaa aattttaatt tcttcttgtc tattatgaat gtccagacgt
    13741 aaggtattgc caataataaa tactacagcc aaagctaata acgccattaa agcatttgct
    13801 gccttgccag aaaaacccaa gatagcgtgc aatcgattaa tccactccat atccagtttc
    13861 gccaacgcga cttgtggaaa agcctgaagt tttcgagata ataaatctaa ttttgcagga
    13921 gaatctataa ctaatgcggg aactacttca ataactgcgg gcaaaggatt ttcaggaaga
    13981 taccgcataa tgtcttgcat accttcttgt tgagttaact cggataatcc atctgctgca
    14041 gatttgatat ttgcctgacc aactccttca gtagtgcgta ctttttcaag taacgacgtt
    14101 tgctccgctt ccggcaatga aggtttcaaa aacagggaaa tgtgcccccc tcgctgccaa
    14161 cgattggtta gttcacttaa attgtcagtg aatacccaaa aaagtgtagg taacgctaaa
    14221 gcgatggcaa taacaatgac ggtcatcatg gttgctatcg gtttgcggca gaaaagattc
    14281 aagctattgg ccgcggcttg caaatgatag gcaagaagag attgtgatcg ttttaacaca
    14341 gccttcctcc tttcagcatg acgatgcgat gtttcattcc ggctattaaa gccaaatcat
    14401 gggttgcaat taatatacta actccaacct gattaaattg ttcaaaaatc ttcattatct
    14461 cagaagaaag ttttggatca aggttaccgg taggttcatc tgccaataac aatgcaggct
    14521 tatgaactac agcacgagca ataccgactc gttgctgttc accaacagat aggtgaactg
    14581 gtaacatttt ctctttactc aacaatccca ccatatccaa agcggcatgc acccttttgg
    14641 caatcatagg ataagcaact ccttgaattt gcaagggcaa ggcaacatta tcaaaaacag
    14701 ttctgtcatt cagtagctga ggagattgaa atgtaatgcc taactggctg cggtgagcag
    14761 caacatctcg ctttttgaga tgatttaatt tcaaaccatt gactgttaat tgaccagacg
    14821 tcggccattc aagaagagca atcaacttga gcaaggtgct tttgccagca cctgaatggc
    14881 cagtaagaaa gaccatttct cctttttgca aagaaaaatt aacttggctt aatgcctcaa
    14941 agccccccgg ataacgttta ctaacctggt caaatgtgat catcatcgaa tatcgcacta
    15001 acaaattgcg ctgcgtcaaa gggcctcaaa tcttcaatgc cctcccctac ccctaaataa
    15061 cgaaaaggta tccccaaatc attcgctata gcaaataata tcccgccttt ggctgtccca
    15121 tcaagcttgg tcatagtaat tccagttaat tgtactgctt catgaaactg ctttgcctga
    15181 gccaaggcgt tctgcccaat actcgcatcc aataccaaca tggtttcatg aggtgccttg
    15241 ggatcgagtt tctgcatgac tcttttcact tttttcaact cttccattaa attgctttga
    15301 gtatgcaaac gcccggctgt atcagcgatt aaaacatcta tttttctcgc tttggcagcc
    15361 tgaagagcat caaatattac tgaggcactg tcagcgccgg tatgctgggc aataacaggt
    15421 atgccatttc tttcgcccca tacatgcaat tgttccaccg cagccgcccg aaacgtatcg
    15481 cccgcagcta acatgacttt ttttccctgc ttttggaatt gtttggctag tttaccaatc
    15541 gtggttgttt taccggctcc attgacacca accattaata tgacaaaggg agagccgtct
    15601 tcagtttcta tggaaagcgg tttctcttcc tggctcaaaa tagtttgcaa atttgtcttt
    15661 agagtcagat aaactgcttc accattagac agttgctttc gtgccaagcc ttcatttagc
    15721 tgtttcaata cgtcttgagt ggtgtccaaa cctaaatcag cactaattaa taaggtctct
    15781 agctcttcga gtaactcctg gctgatttct tttttaccta acagcagccg acttatgcct
    15841 tcccccaatt gatgacgggt tttgctaagg cctttcctaa accgggcaaa aatactttct
    15901 ttgggcgaag gctctttgac agtcagttcc tggggttctt caagcctgga ttccatgctg
    15961 cttttggcat gatcttgttg ctcttcggca tccaaatttg gttcggaatt ttgatttcgt
    16021 ttaaaccatt taatcatttt agtgtacaat tccttaaaaa tatgaaattc tatcatcttt
    16081 ttagctttga ggtgacattg tgcacaaatt actcatttct cttatgatgc tactctcatt
    16141 tcaggctttt agccaagtcc aggagtatac gttaaacaat ggtttaaaag tactggtaaa
    16201 agaagatcat cgagctccag tcgtagtatc catgatttgg tataacgtgg gttcagcaga
    16261 tgagcctgta ggcatcactg gtgtttctca tgccatagaa cacatgatgt ttaaaggaac
    16321 ttccaaatac ccggtagggg tattttccaa aacaattgct gcattgggag gtcaggaaaa
    16381 tgcttttaca aacaatgatt ataccgccta ttatgaaaaa ttagatgctg gccatctggc
    16441 tacaagcttc gaattggaag ccgatcgcat gaataattta ctgcttaata gcgaggaatt
    16501 cgctaaagaa attaaagtca ttcaagaaga aagacgttta cgtactgaca ataacccgca
    16561 ggcacttgct ttcgaacgtt ttctggccac agcgcatctt acagcgccgt acaatcaccc
    16621 ggtgatagga tggatgaatg atttgaaaca aatgaaagta gaggatttaa aaaaatggta
    16681 cgaaagttat tacgcaccaa ataatgccac cttggtagta gtaggcgatg tgaatcctga
    16741 gaaagtacat gccttggctg aacattattt cggctcaata gccaaacgtc ccatagcgtc
    16801 tcgtaaaccc cagcaagaac cctctgcgct tgggaaaaaa atggtttaca ttaacgcgcc
    16861 agcgaaactg ccattactgt taattggcta tactgtacct agtgtaaaaa ctgctaaaaa
    16921 taactgggaa ccttacgcct tggaaattat cgccgggata cttgatgccg gggaaagtgc
    16981 gcgctttgcc aaacacctgg tgcgaggcaa tcaagtagcc actggtgcag aagcctatta
    17041 taacctctat tcaaggtacc aaagccaatt tattgtctat ggggcgccta gccaagatca
    17101 tcaaataaaa gatctggaaa aggcattaat tacggaattg gaagccttaa aaaaagcgcc
    17161 tgttagcaat caggaattac aaagggttaa aaatcaaatc atagcacaaa aaacttttga
    17221 aaaagattct atttttggtc aggccatgga gttgggattg ttagaaacaa taggtctggg
    17281 ttggaaaaat acggaaacat acaccaaagc catcaatgaa ataacccctg agcaaattca
    17341 gcaagttgcg caacgatatt ttcaagaaaa taacatgact gttgcagaat taaagccaat
    17401 caggcaagaa gaggtaagac catgagtgtt ttaagagctt ttatcatttc gtttagtgta
    17461 attttttctc acaatctatt cgccaattca ttcaaaactg aaaaatggca gactaaaaat
    17521 ggggtgcgag ttgtatttta tcaagcaatg gaagtaccta tgcttgatat tagtttggca
    17581 tttgctgccg gctctgccta tgacggtaag tattttggtt taagcgcatt aacaaccaat
    17641 ttgattaatc aaggcaattc cggtaaagac gcaacgaata ttgcggaagc tttagcagac
    17701 accggagcgc aatttaatgc cgaaacgagt agggatatgg ttgtccttag tctaagaact
    17761 cttaccagca aagaggcttt gcagcaatct accaaaacct ttagccaaat tattagtcat
    17821 cctgattttc ctaaagaagc cttcgcaaga gaaaaagatc aacttctcat ggcagttgaa
    17881 caaacagaag aatcgccaga tgatgtagcc atccagaact tttttaaaac cttatatcaa
    17941 gagcatccct atgcccatcc tgtgcatggg acagttgagt cactaaatgc cattaaagaa
    18001 aatcaagtca ttgattttta taaaaaatat tttgttgcta aaaatggtat attagttatg
    18061 gttggtgcta ttgacagttc tcaagctcat caattggcag aacaattgac ccaggatcta
    18121 cccgcaggag agccagcacc aacaattccc aaagcctctc agctggccga tgcggaaaaa
    18181 attaatgtac cgtttccttc ctcgcaaaca gtagttcgct tgggacagat aggaatagat
    18241 catcataatc aaaattattt ccctttaatg gttgggaatt atattttagg tggaggaaca
    18301 ttagtttcca ggctgggtac tgaggtaaga gaaaaaagag gcttaactta cggaatcgat
    18361 agtcagtttg tccctatgct aggtgaagga ccttttatca ttagtttatc aaccaaaaac
    18421 tccgaggcca gaaacgcact gcacattacc caggataccc tgataaaatt tatcaaaaat
    18481 gggccgaatc aggaggagct ggcttcagcg aaacaatatc tgacaggcag tttccctctc
    18541 tctctgggaa gcaataccaa cattgcaaac ctgttattaa gaatggcgtt ctaccattta
    18601 cctgataatt atctggatac ctatgttgct aaaatcaatg cggtaaccga tgctgaaatc
    18661 aaacaagcat tccagcaaca ggtgaatcct gaaaaattac tcttggtaac ggttggacaa
    18721 tcttgaaaca ggtaataagg attattggag gattatatcg ggggaaaaaa atacatttcc
    18781 ccgatgtaga tggcctgagg ccaacaccag acagagttcg ggagaccctc tttaattggt
    18841 taatgaatga tattaaaaat gcccgctgtc tggatgcttt tgcaggtagt ggcgcgttgg
    18901 gatttgaagc ctattccaga ggagcgtctc atgtcgtttt tatcgaacaa tctcccaaag
    18961 ctcatgccag tttgcaaaat accattcgtc aatttgatac ccccaaattg caccttataa
    19021 aaactgatac gttaaattat cttaaacaaa gcaccgagca attcgatctg atttttttag
    19081 atcctcccta cgctctcaac tatgttcctc agtgtcttga tcatatcata acaaataacc
    19141 ttcttgttaa aggaggtctt gtctacattg aatcatcatc tcctattgaa gtaaaaacag
    19201 aactttggaa gcaattgaaa ttaaaacaag ccggtcaagt catttatgga ttatttgaaa
    19261 aactctaaca aacaatgtct tgacaacaag ccttcctcct gtttcaatca aactaatatt
    19321 tccaaatggt aaagaacatt gaatgaacaa caataagatt gtcataatgt ttattttttc
    19381 agcgcttctt gctggttgcg ctggaacaat gaaatttaaa aaaccaccca tcaataatcc
    19441 aagtgatgat gcgaccatta aattagcaga agctgccgtt tcagtcagtg actccatgct
    19501 tgaaatggca aaagttgaaa aagtaattac tccaccaagc aaagacaata ccttaactat
    19561 acctaatgct tataacttgc aagcaagagc cagtgtcgat tggtctggtc cgattgaaga
    19621 attgactgca cgtatcgcca aagcagcaca tttcagattt cgtgttttag gcaagtcgcc
    19681 ttcagttcca gttttaatca gcatcagtac caaagatgag agtctggccg agatccttag
    19741 agatattgat tatcaagctg ggaaaaaagc cagtattcat gtgtatccta acagtcaggt
    19801 cgtagaattg cgctatgcga aaatttattc ttagtttatc tattttgcta tcagccctcc
    19861 tggtagcctg ttcttctcgt aatcattacg gggatactgg ctcattggca ggacttcaag
    19921 ctatggctga ttcaaaatac acccgagctc aaaaaaaaca aaaaatgggt aaaattcgcg
    19981 agatggccct taaggaaacc gctttaagtg ttggcgccca ggctgggtta gcctggagag
    20041 caaaaataat tgacgagcaa ttaaacaaac aggcaaggaa tcttgatgca atttatgatt
    20101 ttaattccct ggtcttagag cataatattt tgcctcctgt attacttgag ggaaggaata
    20161 ctttgaatct tgcagatgct caaagtatcc gtatttcaga ccgcacctat aaagtcgcaa
    20221 aacaagctca tttcattact actccaccta cctggcggca atatttgtgg atggattatg
    20281 tgaaacctga agctcccaat gtcactctac tccccaaaac caaggctgaa aaagaaatat
    20341 ggtgcatcta tactgaaaga gggtggaaaa atggtataga tcaggcaaac accattttgg
    20401 aagaaaatat tgcccgcatt aaagaagatt tcggcggtat gattctttat agaaaattac
    20461 tggctatgaa catggtatct cctccttatg tttctcatac tgacttgggc gtaactgggg
    20521 atggctcaga aattcatatc gatgacaggg tcttacgcat cacggcgtta cctgaactta
    20581 atgtcaatag tgcagaatgg agagctgcgg tcgccaaaga tgaaaatgct ctcgaacgtt
    20641 tcaaaaatat ggaaaaactg gcaaatcagg ctaaaattgt tataacaaac aaatcctggc
    20701 aacctattat agcaccagta agttaatgtc tatggataat attaatttaa tgcccgatga
    20761 gccaactcgt ttcactccag tattcatgga cagaatgctg gagcatgcag aaagcctgaa
    20821 tgcttcagat ataacaatcc aaactggcga acccattttt gcagaggttt atggaagact
    20881 ccttaaaatt acaaatcgac gtctttccaa tacagaattg ggcgacctta ttaattccat
    20941 ttatggcccg aatgccacga cacaattact ttccggtaaa gatatagata ctcattatga
    21001 gtttcgaccc aaccgtggtg tacgctatcg atatagggtt aacgcgactg cctgcctggt
    21061 tgaaggtcat gatgccattc aaatcacatt aagaactatt ccaaccacgc cacccaaact
    21121 cagtaccatg aacctccctg ataacattat tgaagcgatt gctccacagg aaggaatcgt
    21181 gtttattaca ggcgctaccg gttcgggcaa atcgacattg ttagcttcca ttattcgaga
    21241 acttattgaa acatcagact caaaccgtaa agtattaact tacgaatccc cgatagaatt
    21301 cgtctatgat gaaattgaaa caatttccgc tgtagtaagc caatcagaga ttccaagaca
    21361 cttacccaac tttgctgatg gagtaagaaa cgcattaaga cgaaagccaa gattaattat
    21421 ggtgggtgaa tgtcgtgatg cagaaaccat cagtgccgct cttgaagccg ctttaacagg
    21481 gcaccctgtc tacacaaccc tgcatacttc tggtgttgca gaaacaatgc gacgtctggt
    21541 cacatctttt agcggggaag agcgtttggg aagaaccatc gatattcttg aaacaattag
    21601 gctttgcatt tggcaaaaac tggtcccaac cgttgatgaa agacgtgtag ccctaaggga
    21661 gtatctggta ttcgatgaag aagtgagaga tattctattg gagggagacc ctaacgaagt
    21721 gacttcagcc actcgtaagt tagtgaggca aaaagggcag ttaatgactt gggacgcaaa
    21781 aatgaaattt gaacagggga ttatcagtga gcgagtttat aaattaatca ttgctggggc
    21841 caaagaatat caacaataat caaaattaat ctgtttgcac ttgccataga aaaatcattg
    21901 atataccgca tataaaacaa tcaagctcaa ggattcgatt catgatgttt cacaggaaaa
    21961 gcctatatca ttattttaac ttatttattt tgttaatttt aggctcttct ctcaattggg
    22021 cttccgtcaa tcagcacagc acagcttcgc caaccctcaa cattaaaatt ttaggcatta
    22081 atgattttca tggtcaaata agcagtggga gagccgtaaa aaatgaacct gttggcgggg
    22141 cagctgtcct ggccgcttat cttaaagaag ctcaattggg aatggaagat cgatcaataa
    22201 ttaccatcat gggtgatttg gttggagcct cccccccttc ttcaggactg ttgaatgatg
    22261 aacccagtat tttatttatt aatggcttgg gaaataatca atgcaagccc gaatcaagaa
    22321 tggatcctct ttgcaacatc gttgccacag taggaaacca tgaatttgac aaagggcaaa
    22381 aggccatgtt tgatttaatt tatggtacca ataatccacc gaccgattca tggatctcat
    22441 tacccaatta tccgggtgca tcttatccct atgtttctgc gaatatcgtt gacgccaagt
    22501 cagaacaacc tctcttccca ccctacataa ttaaacggat acataatact cccatagcat
    22561 ttattggcgc tgttttaaaa gatgcggcgg actctatgtt tccagcaaac gcagaaggag
    22621 ttaaattttt agatgaagca gacagtatta atcattacat cccggaaatt aaagcgaaag
    22681 gagtcaatgt tattattgtc ctgattcatg aaggtggaaa tcaaattcct tatgagggag
    22741 acacccaaac tacctcagca gtagaaggtc atattaaaga aatagtttat tcccttggtg
    22801 atgaaatcga cgttgttatg ggtggtcaca cccatcaatt tctaaatgct ttccttccaa
    22861 atcgcaatgg gcataagatc ctggtcacac aagcgaatag ttacagtgct gcttttgctg
    22921 aagttaccct ccaaatcgat accaaaaatc atacggtaaa aaacaaatca gccaaaatta
    22981 ttacaactta tgctaatcga tggcctggaa caaatccgga tacacaagcc ttggaaattg
    23041 ttaaattagc agaagataaa attgggccaa tgataaataa ccatgtaggc actgcagaat
    23101 atgatctgct aagaaaacaa aataaagatg gtgaatcgag tttagggaat ctgatcgcag
    23161 atgcttatag aacagtaatg aataccgatc tgggtatcac taatcccaat ggaattcgtg
    23221 atgatttaaa agctggggaa gttaactggg gaaatatcta ttctatttta cccttttcca
    23281 acaggatagt cacaattacc ttaactggaa atgatattta tgacttgtta gagcaacaat
    23341 ggatgggctc ttatatcaat atgttacaaa tttccgggtt tgcctacacc tatgattcac
    23401 atcaacctct gggacataaa ataattgaaa ttagacatca aaataaacca ttgcttagag
    23461 aaaagatgta taccatagcc acttcagatt ttcttgccag cgggaatgga gttttttcag
    23521 ttatgaaaag aggaaaaata atccatattg gtcaaagtga tcatgacacg gtaatcgctt
    23581 atattaaaag tttacctcaa ccctttcacg cgtccattga ggcaaggata aaagaatcag
    23641 agactggatt aaaaacagga gttggataga ctaaattcgt tttaccgtca gcccctgttt
    23701 ttattattta ttaattaagt tttgctatta ttttatgcat tctaatccag cacatagtag
    23761 aatttgtttt cgctaagtcc tcaagttcat tatgtaattg tttgatttcc tcccttttag
    23821 ctatgccaag ctctaaaagc tgaggggtca aatcatcaag cgccattgaa taaattagtt
    23881 tttctttgtt cgttctgagc aagggttgaa ataaagaaca atgctcaatt tcaaaaccgc
    23941 aggatgaaaa ctcttgctct aatcgatgcg ctatctgaaa atcccgattg cttttgctaa
    24001 agcatgacct cgcccattgt gtaaactggt cataggaggg tgaaggagga gagcaaaagg
    24061 tagaatcaaa taaagagggt tcttcacata cgacgaaacc accagaatta agacaatttt
    24121 tcatttggag aatgacagag cgcgcatcag ccacatgatg taaaaccatt cgacaataaa
    24181 tcaaatcaaa tttttcccca agatctgaaa gatgattaac atcccacacc ttaaatcgta
    24241 tattgctata ggttctggtt cgttgttgag tatgatccag ttgatcctga ctcaaatcta
    24301 tagtaagcaa actgccctcc cggccaatta atttggctaa ttcgagggtc atatcaccag
    24361 agccacaccc tatctccaga gcagtcattg ccgctgaagg cccaacctca gaaagtaaat
    24421 ccaggctgct cttggcatac aattgatgtt gcagagaaag acgctctctc gccttgtcat
    24481 tggtatttag aatgtaagta ctgggcataa tatatcctta tatttgataa aaaataatta
    24541 ttgttcttca aaaagagtta catggctttc cctttgccac aaagactcat ttaaatattg
    24601 gtttaaatgt tttgcctgta attgaaacat ctggtttaaa ttttctttag ttgagctacc
    24661 catatgtggt gataagacga cgttatcaag ttgccttaaa gcgaaaggaa cttccggttc
    24721 atactggtat acgtccaaag cggcaccagc gattgtatgg cgttgcaaag catcaatcag
    24781 agcgttttga tccactatac tgcctcgcgc aacattaatc aaatatccct ctggccctaa
    24841 attatctaaa acctgtttgt taattaaatg ttgtgtatca ataccgccgg aacaacaaat
    24901 gattaaaaaa tcactaatag atgctaaatt tgcggctgtt ggacaataca agtaagggct
    24961 atttttttga cttcgggccg tataagcaat ttttagcccg aaaggctctg caaattgcgc
    25021 tatcttttca ccaatttgac cgaatccaac caagcccaat tgctttccca ataaatggtt
    25081 gcctagaaaa cggggctttt tctcaaccca ttcattattt cgggtatagc gatcatttag
    25141 aatcaccctt cgcgacagtg ttaacaataa agcgatagca agttctgctg tgtcatgaac
    25201 acctgcatta ggttgtgaat gcagaataat atgattttgc tttaaaaaat taatatcaat
    25261 attgtccgtt cctattccca ggtgactaat aattttcaag ttaggaaatt gcattaaaaa
    25321 cgaatggtca atctgatccc ataccgttgt ggctaaagcc acaacccggg attgatccac
    25381 tcgtgaagcc attttccaac catgaataat gttccaatca ggaatcagca tgggaataat
    25441 tggctctaga aattggttgg tcaggtaaac aaatggcttc attaaaatgt cctatttttc
    25501 agaataaaga ttccgtgtgt tgcccatgat attctctctc atctttctga caagcaaata
    25561 aaatcaagcg gtaaaatgag cgttctcaag tagagacata tagcgattat tacggttatg
    25621 atactatatt cttttactat tgcatttcat tattccaagg agacgttacc atagagagga
    25681 atgcacagca atattttgaa agcgcgaagg aattgttctt gccagtaata cctgtggagg
    25741 agtttgatgg ttttatactt aaattgggca agaataacta ccatttttgc aaaaatgaaa
    25801 ctccttttaa taatagctgt agtgccaata ttgctattga taaatattgt acctctaaac
    25861 tattggaaaa agcgggtatt cctgttccca aagctatctc tttaaactgt agtgaatttc
    25921 aacggaattt gcataaagat aaaattgcac aattaaactt tcccttagtc attaaaccaa
    25981 tagacggttc gctgggaatc ggtgttttat gcaatataaa aacatgggaa gaactggaaa
    26041 gccatttaac aaaatatttt tcctcttatc agtgcttaat aatagaagaa ttccacggca
    26101 aactgaattc ttaccgtgta ttggttttta ataaccgtat ccttggcata gtccaacgtt
    26161 acccagccgc tgtaactggc gatagagtac atcccataca tgagttgatt aaacaagaaa
    26221 atttaaacag aaaaagaatc aatgaagctt taggtgaaat tactttagat gaggaagcgc
    26281 ggattaaact tcaagagctg ggcataacag aaaattacat cccctctttt ggagaacaaa
    26341 ttgttttatg ctatacaagc aatgcaacaa gaggtggatc ctatgtcacc ataaacaaga
    26401 agatgtgcaa gcaaaatcgt aaattattgc ttcaagcagc caaaatactc gacttgcaat
    26461 tggttgggat agatgtggaa tgttcggata tcatcaatat tcctatagaa caatcaaatg
    26521 gcgtcatact ggaagtgaat cataagccca gtattcgaat tcatgagttt ccaatgaaag
    26581 gaaccccaca aaaagtcaca aaaaaaatta tgcgcagttt catcacccgc catccgttgt
    26641 cttacttata ttctatctac aacaaccaac cgaccgcttt ttatgtccgc agctctattc
    26701 ttattatcat tatgggatta attcttttag ccgtgtggta attcacctca cctaaaactt
    26761 atgtcatttc ccaatcttta cttccaatac ctggcagtga gcttatttga taataaattt
    26821 atttctgaaa tcactttgct caggtaaagg tagttcactc catgataaat tctgataatt
    26881 caccaatctc aatgaaagaa atttgagatt aaagtgctta attgaacaac atattgaaca
    26941 tgctcatttt gcaagtggtc ttttaaagtt tatacttaat aataatgagg ttaaattatt
    27001 attacgaggt attaaatgaa tcctgtatcg attttagatg cctcattaag agatggtgga
    27061 catagaacta attttcattt taaggatgat gatttaaaaa aaatactact tcctcttgat
    27121 aactcaggta ttgaatacat tgaaattggc taccgtaatg gttcacttca ccctattgaa
    27181 aatataggga aagctggaat gtgtccccaa gattatctcc tgtattgcca atctctaata
    27241 aaaaaagcaa aaattgcagt gatggttcat cctcataatg taaacgaaca ggatctgttc
    27301 gagctaaaaa attgcggtgt gaaattaata agaatatgta tagcaaaagg tgatctggta
    27361 aatgctgtcc ctctgattaa actggctaag aatttgaatt ttgaaacctc agtgaacatc
    27421 atccatattt cttattatac ggaaagcgaa ctggatagtt tgattgagga agtaagcgaa
    27481 cacgaaccgg atatgatcta ttttgctgat tctaatggaa gcttatttcc tgacaggata
    27541 aattgcctgt ataaaaaata cacccgtcaa tataaagttt cttttggatt tcatgctcat
    27601 gataaccttg gattggcgca agccaacgct cttgcggcgg taaacgccgg ggttcatttt
    27661 attgatgcct cactggcagg aatgggcaag ggaacaggta atctaaaaac tgaatttttt
    27721 atcgcttatt tacatgcgaa caatataaaa aaatacaatt tggaagatgt gttgacagca
    27781 gctaattatg taag
//
LOCUS       DACTJE010000024        27275 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.024, whole genome shotgun sequence.
ACCESSION   DACTJE010000024 DACTJE010000000
VERSION     DACTJE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 27275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27275
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.024"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            87..1400
                     /gene="lem29"
                     /locus_tag="I9760_000789"
     CDS             87..1400
                     /gene="lem29"
                     /locus_tag="I9760_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem29"
                     /protein_id="HAT5042576.1"
                     /translation="MGLEKKIKLSQRSLELLNDYLNLGGSGPSNLVNGSSSIDFSSLT
                     QLLASNLSKSSKTSKEIKKKREFIEAALYAELLRDLSTSLNGSDEEIKPAEKEPPVSN
                     KLKFWLLAIAGTLLAACEGFDSITTMMAVLPFPAWVVLCAGILFSALSVVVFYGFDLV
                     QVSQNLEIKLTDTPKLLDLYLSQMEEIKAIRKKIDNYSLASMSADELNELHLILVMLQ
                     DRVNSLVKAGKKFDLALNSPKMDVAKTIVSGVSGILFFGGGFFAGQSVATFMLALVMT
                     SVTPTFWPVLLFSVIVGLAAFSLYWYVQRVGVNKLVSEWFGLDEDKIEKLCGKSKMDK
                     ELKKLNNLDEKVMTTSDLLSKVANLESKLIDIGKQMKEMSTHQAPEKKLEGATGLKAS
                     SNVYSFHFNSSNRDQGSVYGERILDDAVSDYGRGQNHNDSIHSLC"
     gene            complement(1285..2085)
                     /locus_tag="I9760_000790"
     CDS             complement(1285..2085)
                     /locus_tag="I9760_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042577.1"
                     /translation="MNRHDVKAIHSYVVDEEKKKISLKLINKLYRFLSKYYHLASQEE
                     FIRFIIQPDLYGELTVLYGENKEIAGFSRNCRQSVDLGKKQVTCYIAYLYLNPEYQIH
                     ASIKSAGLTQAIKYKLANPQEELVYIAFANNPLTYEFIYQLSDLIYPKPLQRVPDQIL
                     TVLNALKKQNGWISTNNHPMVVNSPLVPLRSQLSRINEESSELKEFYMSANPDYLQGN
                     SLLIYMPLHLANISYGLNHYDSDPYHNQKQHHQGSSHRKRSPDPDLRN"
     gene            complement(2368..3060)
                     /gene="hda"
                     /locus_tag="I9760_000791"
     CDS             complement(2368..3060)
                     /gene="hda"
                     /locus_tag="I9760_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HAT5042578.1"
                     /translation="MNKQLALAIKLNDEATLDDFNWGNNTLLQQQLIEMLSFKADRLL
                     YLWGPKGSGKSHLLQACCQAINLTQSAIYLPLAFLKEWGPQSIEGLEDQTLICIDDIN
                     TIANDSAWEEALFHLYNKIKDSERSLLIISGNQPPIKCDIKLADLRSRLSWGLVIQLN
                     ELNDDEKINTLKMRAAKRGFELPESVGHFLLNRCSRNMHDLYEILNQLDDASWEAHRK
                     ITIPFVKNILKI"
     gene            complement(3063..3617)
                     /locus_tag="I9760_000792"
     CDS             complement(3063..3617)
                     /locus_tag="I9760_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAT5042579.1"
                     /translation="MILKHIPNTLTLLRLVLIAPFLMYLFHHEYVKAFYIFIAAGLTD
                     GLDGWLARHFQWQSFFGSFVDPLADKLLVASSFISLALLNSLPWWLVLLVFLRDFTIS
                     MGVLAWYYFIQRKLDFEPSRLSKLNTGFQLTLVTLCLFELAYFRFPYDLVDILIYLTA
                     FTTVTSYLDYVWTWSRKAWSPKES"
     gene            complement(3877..5604)
                     /locus_tag="I9760_000793"
     CDS             complement(3877..5604)
                     /locus_tag="I9760_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore biosynthesis protein"
                     /protein_id="HAT5042580.1"
                     /translation="MALAYGNFHELSHQLRFLLFEIGIGLPQNSVDYFITLAHKNTLK
                     RLQHASIKEGLIQSAIASHHIHDFIDQLQIKLKNSMPESKFFQWRKIREALDESIANE
                     ALAYAYRQNWNTQLRNEAMHYKSLWTWINNELSPYQTLLFLEQWGSLRHPYHPAFSAK
                     TGFTRREVLQNSPEFQAKVSVHWCALNKTKIQSISSKIDYANQISQEFPKEYFYWREK
                     LLFSHINPDDYYPIPVHPWQWRNQLQMAFASLIDNKSLILLPHHQTLIPSLSPDIMMP
                     TQSTQCTLKLATTLSTSMAGKLDNSDDMVLLSRWIDSLLAKTNYYQNTLFICKNLESM
                     STYDQTLSECNRVKLLFGLYQNPLHKIRQDQRAVPLPALLTDSPCSNTPLLIEIIKAS
                     ELHPTTYFTEYCYKMLFGQLHLLLKYGLALEVEQHNILVIFDDNKPQGIIIKEPNNLK
                     LCNHELFKNVQKPNAPDSLSIYTKDLNQVRTLFIQGTLKNHLHHLIGCLRNEYQISEK
                     TLWGLTRQVMQTVFKDLSKDIDPRILSWQQHLLLHDNWEHQPELLLSLHSKINRNITI
                     KEYNPLSEI"
     gene            5790..7766
                     /locus_tag="I9760_000794"
     CDS             5790..7766
                     /locus_tag="I9760_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAT5042581.1"
                     /translation="MNYRPDIDGLRAIAILLVLIYHGGLSLFPSGFIGVDVFFVISGF
                     LITTIIHESLNKNDFSFFDFYNRRLWRLQPVFVALIVVTTLLSVLFYLPDDLIEFSRS
                     ARKASLFISNLFFNKTTTGYFSPDTHQLPLLHTWSLSIEWQCYLVLPLAMYGLYRFLN
                     KGQVIYIVHGLAILFFLLALYYSQAAPAQTYYQFSSRIFEFLIGSCIALSPRIEFSIN
                     KSILVILGLSALFFIIYTATLDNILLGYPNWRTLFVCLATGLLIALGTNFPSLAIVNA
                     LSFRPLVFIGVLSYSLYIWHWVVFSILRYQNIEERSEVLFIVYGIVFVLAYLSWRYIE
                     KPTKKFRHIPFRYTVVILLLIPVLFSHLSSLVIKSHSGYPQRFNQELVRVYKQLKSYE
                     SPYRALCIDNTRADNAWKCKIGAKEQGGRTGLMIGDSFSNHYWGFMDVLGKAAGVSIL
                     AQGTSSCISLPGIYLYDWWHFKDKIYQDCYEQTEKYYRMIQKNHYDYVIIGQVWENYV
                     SAHIINQRGDDYSAELTKKRLADALDRALGVIISSGAKPVLIESTAVTHGNLHQCFFK
                     HIKLRQSYNSEECRFTLTSSDNETWLNQLFDNMRRKYPQLIIIDPKQVQCRDNVCYSD
                     LNGIPVYRDEGHITDYASYQLGYLYLREFENPLI"
     gene            complement(7817..8083)
                     /gene="yhbY"
                     /locus_tag="I9760_000795"
     CDS             complement(7817..8083)
                     /gene="yhbY"
                     /locus_tag="I9760_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAT5042582.1"
                     /translation="MDTSFKQSLKAKAHHLKPVVLLGAKGLTEAILAETNVALLAHEL
                     IKVKINGAEKEDRMQMAEELCEQLHAELVQMIGNTLVLYRKNEE"
     gene            8186..8806
                     /gene="rlmE"
                     /locus_tag="I9760_000796"
     CDS             8186..8806
                     /gene="rlmE"
                     /locus_tag="I9760_000796"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAT5042583.1"
                     /translation="MNRTKSSKRWLQEHFDDVYVKKAQAEGYRSRAVYKLKEIDDKES
                     LIKPGMTVVDLGAAPGGWTQYASEKMRGSGRLVALDILPMDALPNVEFILGDFREDNV
                     LQELINLIPQRTLDLLLSDMAPNMSGSSAIDIPRAMYLVELAFDFAEKMLKPGGNMLV
                     KIFHGSGFDELVKQARASFEKVVIRKPSASRSRSKETYLLAKGYNL"
     gene            8928..10838
                     /gene="ftsH"
                     /locus_tag="I9760_000797"
     CDS             8928..10838
                     /gene="ftsH"
                     /locus_tag="I9760_000797"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAT5042584.1"
                     /translation="MVKNLFLWLIIAIVLVSVFSNFGPRNSVAEKISYSQFLQEVDQG
                     MINSVTIEDNKIIKGVTKNNKRFVTYMPMQDNALLGELLKSKVDVSGQEKQQESFLLH
                     LFINWFPMLLLIGVWIFFMRQMQGGGGRGAMSFGRSRARLLGEDQVKVTFADVAGVDE
                     AKEEVKELVDFLRDPTKFQNLGGRIPRGVLLVGSPGTGKTLLARAVAGEAKVPFFTIS
                     GSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTL
                     NQLLVEMDGFEGNEGVIVVAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQILKV
                     HLQKVPVDSHVEVKAIARGTPGFSGADLANLVNEAALFAARANKRKVGMIELDKAKDK
                     IMMGAERRSMVMDDNEKKLTAYHEAGHAIVGLSVPEHDPVYKVSIIPRGRALGVTMFL
                     PEQDRYSHSKRRLESQLCSLFGGRIAEELIFGPESVTTGASNDIMRSTEIARKMVTTW
                     GLSALGPLTFGEEEEEIFLGRSVNKHKEMSDRTAQQIDDEVRAIIDRNYQRAKEILET
                     NIDKLHLMAQSLIKYETIDTNQIQEIMSGKEPTPPEDWGSTKPMDKAESVNDAPAKPI
                     NGETIENPAEGH"
     gene            10871..11746
                     /gene="folP"
                     /locus_tag="I9760_000798"
     CDS             10871..11746
                     /gene="folP"
                     /locus_tag="I9760_000798"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAT5042585.1"
                     /translation="MNSEQFLGWLERKSQPPSSLVFEKPLIMGVLNVTSDSFYDGGKY
                     LSVDSACDQALKLIACGADLIDIGGESTKPGAPPVPLDVELSRVLPVIKQLRVLADVC
                     ISIDTNKPEVMEAAIDAGANVINDVYALRRDGALNVAAQLDVPVCLMHMKGEPRTMQE
                     NPSYPEGIMKELQHFFKERIDKCQKAGLNKNKLILDPGFGFGKRVKDNLKLIYHLNEL
                     RSFGLPILLGVSRKSTIGAVLNNEVEQRLIGSITLGIYAALKGTAIIRTHDVDETNQA
                     LTMINAIYQAAKEIG"
     gene            11810..13177
                     /gene="glmM"
                     /locus_tag="I9760_000799"
     CDS             11810..13177
                     /gene="glmM"
                     /locus_tag="I9760_000799"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAT5042586.1"
                     /translation="MSQRKYFGTDGIRGHVGLSNINPEFVLKLGWAVGCVLANGARKK
                     VVIGKDTRVSGYMLESALEAGLSAAGVDVALLGPMPTPGIAYLTQTLRANAGIVISAS
                     HNLFEDNGIKFFSADGGKLPDSVELAIEAQLEKQLQTVPSAKLGKATRINDAAGRYIE
                     FCKSTIPSLSRLSNLKIVVDCANGATYHIAPNVFSELGADVVPIGIKPDGFNINQECG
                     STAPELLREKVIAVGADIGIGLDGDGDRVILVDSLGNLVDGDQIIYIIAKDRHQRGVL
                     HGGVVGTLMSNYGLELAITSLGVPFQRSKVGDRYVLETLREKDWKIGGETSGHIVCLD
                     KTTTGDGIVAALQVLSIMVKQNKALHELTAGIQLLPQTLVNLKTNNAALLASNPDVIQ
                     AVKNLEKHLNGEGRVLLRPSGTEPLLRVMVEGANASIVKQQAQMLCDDISQIDKKMTE
                     SLPST"
     gene            complement(13246..16701)
                     /locus_tag="I9760_000800"
     CDS             complement(13246..16701)
                     /locus_tag="I9760_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="interaptin"
                     /protein_id="HAT5042587.1"
                     /translation="MNEIILHLMNEISTLTPPINPLNLKQIFSSTLLPEGKAPVEVES
                     QLNLLLLIKKYVDEYSSLAEEQEEYKRKKHKLDEVMKKGEELQRKISDLAKKIEHAKN
                     ELNPLYKEIEPKANEVLSKQNPLAPPYKFPEYGEVQKSIVVGHYSYLFKTSKEIETTR
                     AKLKLVLLKNKWNALMADLSQFGIQPPVEEDAVDSLQKFIAAVELEIKKLTRACEKWD
                     ELQKRFAKQLSATDINKKETELEKQIELLLKDLGRIETVVTNHSLAPDLKAELTRKFQ
                     ESEDTQGLIQEYQNQIDGLLSNFNPSSWLSWYSDSNYSDNQQKLKNSVSFLQLLAQQK
                     ELKIKHHELVKQQELLIKAIPETPSNEEDISIYKKLVPDAIDLINEIPIESIPNFVLP
                     SGLSYDSSPADFYLVLLILMPKVSEKKEQFSQALEKLQEMPALGKQIQQLRSGYDLPI
                     TMDVQLPSLQEAEESKDSLNEEDPLKEELKSQIKLCQSYLETAKQIDLLMKDHGQTTN
                     EAKALIEQLRLISNQPLKNEDINSIKEDLSTLANQIQSLVSELNQLPLPNLSGEKIDP
                     PLEKQEIAVDKTIPLTVELMHPAQVIQIGNDSPIEKQQLDNEEETPVVLESLRPRPVI
                     HKESESITQKLQVDNGQEMPVVLDSTCTPQAIHKELESTTEKLQVDNGHEIPVVLEST
                     RSPQIIHTESESVGKKQHVEVEQEIPVTLELIRPSSMVEKDSVATVEKQQVTERQETP
                     IVLESTRLSPMVQKDTGLSGEKQHKEIEQEISVVTELTPHTQVVRQGSESSLEQQVPN
                     GQETLAVVKSSPSEPLRPSTPEVPAISRKPNGLSLFNGHDELSEDNILAFFDEAGNQI
                     SISSEEDSETFTVDRGKVISSGIDKAFKEKPVSTGHELDKLSNPQHQESAPSISPLPP
                     SSILLKQKLDSFHVQNMEYIKQHSEEIQLWYKGLYDAAQSSCVNEALGLKALHLLKDI
                     LFELKNQNDLSVLLAYKRMCPNPLQDIQNILRLKPALPIVDESIDEEQQLKNWPEELQ
                     KFHQQYVKLKKEHPLEAELFIQAIHSLISIKHLMELPDAKTSNREAMPLITQDPRYEP
                     LKRHRGFIRAWEYIEDFFRMLIGKLTGQDEYEYSKRPCFFKTRSHRLLEEVDTILHSM
                     APTSS"
     gene            16945..17694
                     /locus_tag="I9760_000801"
     CDS             16945..17694
                     /locus_tag="I9760_000801"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAT5042588.1"
                     /translation="MRQKIVAGNWKMNGQIQQVTELVSQIEELIGFDCAAQVAVMPPS
                     IYIPKVRDCLRTGKIVVGAQNVYPKDYGAYTGELSAPMLKDFDCRYVLVGHSERRQFF
                     HEDENFVAQKFHHVKDHGMIPVLCVGETLSERENGKTEQVIAQQVLAVSAKGKDCFRD
                     CVVAYEPVWAIGTGKTATPEQAQKIHQFIRDLVGEINDSDAKHLTLIYGGSVNENNAK
                     ALFSMPDIDGGLVGGASLNAKQFVEIVKCIN"
     gene            17682..18005
                     /gene="secG"
                     /locus_tag="I9760_000802"
     CDS             17682..18005
                     /gene="secG"
                     /locus_tag="I9760_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAT5042589.1"
                     /translation="MYQLILMIHVLIAVILIGLVLIQHGKGADIGAAFGSGASNTLFG
                     SQGTGGFLFKLTGGLAITFFITSLMLSYMVSTQNQKAGQLAIPQQTGAPVSSVPVPVE
                     KNNKK"
     gene            18076..18160
                     /locus_tag="I9760_000803"
     tRNA            18076..18160
                     /locus_tag="I9760_000803"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18110..18112,aa:Leu,seq:gag)
     gene            18185..18541
                     /locus_tag="I9760_000804"
     CDS             18185..18541
                     /locus_tag="I9760_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HAT5042590.1"
                     /translation="MLSQYLPVLVFIIVALILGAVMIGAGSLLSKHSPDSAKNSPYEC
                     GFGAFESSHIPFDVRFYLVAILFIIFDLETAFFFPWALALRKIGWFGFYAMMLFLGLL
                     VIGFIYEWKRGALEWE"
     gene            18791..19267
                     /locus_tag="I9760_000805"
     CDS             18791..19267
                     /locus_tag="I9760_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAT5042591.1"
                     /translation="MAVAELEKKGFELTTVEKLVGWARSGSMWPMTFGLACCAVEMMH
                     VGAARYDLDRFGIIFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMPEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIRRKPVLEA"
     gene            19290..19973
                     /locus_tag="I9760_000806"
     CDS             19290..19973
                     /locus_tag="I9760_000806"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HAT5042592.1"
                     /translation="MTKNEYLIEKLQADLANHITELTSAYGEVTIECEVQNLLPVMIE
                     LRDREEFSFDQLIDLCGVDYLHYGDYDWETESATEHGFSRGVERQEAKAYAVNKPRFA
                     VVYHLLSTKKNHRLRVKLFVEESHLIVPSVHHLWKSANWFEREAYDLYGILFDGHPDL
                     RRLLTDYGFIGHPFRKDFPLSGEVEMRYDAKLQKVIYAPVDIVPRIVVPKVIRNDNRY
                     IGNEGSKND"
     gene            19966..21219
                     /locus_tag="I9760_000807"
     CDS             19966..21219
                     /locus_tag="I9760_000807"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HAT5042593.1"
                     /translation="MIELKNYTLNFGPQHPAAHGVLRLVLELEGETIVRADPHIGLLH
                     RATEKLAETKPYIQSIGYMDRLDYVSMMCNEHAYVMAIEKLLGIEPPLRAKYIRTMFD
                     EVTRILNHLLWLGATALDIGAMTVFLYCFREREDLFDCYEAVSGARMHATYYRPGGVA
                     RDLPDTMPQYKPSRWHSEREIEKKNHNRQGSLLDFLWDFTERFPHCVDEYETLLTDNR
                     IWKQRTVDIGVVSPENALQWGFTGPMLRGSGIAWDLRKKQSYAAYDRVEFDIPVGKTG
                     DCYDRYLVRVEELRQSNRIIRQCIEWLRKHPGPVKVDDYKVSPPRRVVMKHDMEALIH
                     HFKLFTEGFCLPRGEVYSSVEAPKGEFGIYMVSDGANKPYRLKIRAPGFAHLSSFDDM
                     VRGHMLADGVAILASQDIVFGEIDR"
     gene            21280..21783
                     /gene="nuoE"
                     /locus_tag="I9760_000808"
     CDS             21280..21783
                     /gene="nuoE"
                     /locus_tag="I9760_000808"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAT5042594.1"
                     /translation="MSANSAKILSQLMPANRMLDIDHWISKYPKDQKQSAVMSALRIV
                     QEEHGHLTTELMNAVAEYLDMPPIAVYEVASFYTMYEHKPVGRHLINVCTNISCMLRD
                     SAGVVEHLEKKLGVNLGGTTEDGRFTLRSVECLGACVNAPMMQVDKDYHENLTAESID
                     KVLEQYQ"
     gene            21802..23079
                     /gene="nuoF"
                     /locus_tag="I9760_000809"
     CDS             21802..23079
                     /gene="nuoF"
                     /locus_tag="I9760_000809"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAT5042595.1"
                     /translation="MVELNQVCYRTFHLPESWSLSTYESIGGYSAWRKILKEQTSPQL
                     IIEELKTSALRGRGGAGFPTGLKWSFMNRNSPVQKYVVCNSDEGEPGTCKDREILTLN
                     PHQLIEGMAIAGYVMGATVGYNYIRGEFWLPFERTEAAIKEAYDAGLLGKNILGSGFD
                     FDLYNHLGAGAYICGEETALLNSIEGKKGMPRFKPPFPANYGLYGKPTTINNTETFAS
                     VPVIMEKGGEWFLKLGKPNNGGAKCFSVSGHVNKPGNYEIPLGTPFKTLLDLAGGVIK
                     GRKIKAVIPGGSSMPVLPGDVMLGLDMDYDSIQKAGSGLGSGAVIIMDETTCMVDALY
                     RISEFYMDESCGQCTPCREGTGWLVRLLHRIINGHGEPGDVEKLVNVANNIEGRTICA
                     LGEAAAWPVQSFVKHFYDEFEYFIKHKRSIVAA"
     gene            23096..25447
                     /locus_tag="I9760_000810"
     CDS             23096..25447
                     /locus_tag="I9760_000810"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HAT5042596.1"
                     /translation="MATIEIDGKTFEAENGKMIIEVADEAGIYIPRFCYHKKLSVAAN
                     CRMCLVEVENGRKPVPACATPITNGMKVFTKSEQAIHSQKVVMEFLLINHPLDCPICD
                     QGGECELQDISMGFGADKSEYTETKRAVDDENLGTLISTEMTRCIHCTRCVRFGEEIA
                     GVRELGATGRGEKTQIGTYVEHSMTSEVSGNIIDLCPVGALTSKPYRFKARAWELTQH
                     DSVAPHDCLGSNVHIHTRNNKLMRVVPRENESINETWLSDRDRFSYLGLNAVSRASKP
                     QIKKNGQWEAVDWETALKFAAEGISRVIKQHGPEQMAAFASSSSTLEEMYLLQKLMRE
                     LGVQNLDHRLQQIDFRDQAFLPTTPVSTLPYAEIDHQHSILLIGCNIHREVPLAGTRV
                     RKAFRNGAKIYALNPVDFDYHFDLSGRVIISPLEMPMQLAKLALALTTELASLPEEVQ
                     KLLIGLEVDKQTKQIAQSLKEEKACLITGAIVENHPEASLLRTLVAIVQKLSGAKLVR
                     LTTGANSAGACIAGMLPHRTVAGKSIAEPGFNVQEALNSKLKGYLLMGVEPGYDFANP
                     AGARQSMLAAEFVVLLSAYEHESMHDYADVILPIAPYAETSGTYINIDNTWQTVKGAM
                     LPFGESRPAWKVLRVLGNLLHCKKFDYTSTEDILEEVKEAVSMTMEHEYEPYYPESLP
                     VINQSLVRVGEWPLYRIDAITRNAKELQLCAASESACIRIHPSTADRLKLEEIATVSQ
                     GDIEITLPLKRDERIAVDVVWVANAMPETVDLGHSFAAITIKR"
     gene            25460..26482
                     /gene="nuoH"
                     /locus_tag="I9760_000811"
     CDS             25460..26482
                     /gene="nuoH"
                     /locus_tag="I9760_000811"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAT5042597.1"
                     /translation="MLHTIGILIWIIIKILVIVVPLLISVAYLTYAERKVIGYIQVRI
                     GPNRVGLKGLLQPFADLLKLITKEIIVPTRSNKYLFVIAPLFALVPSLVGWAVIPFQE
                     GIVLANINAGVLYLFAMSSLGVYGVLIAGWASNSKYAMFGALRSTAQTVSYEIAMGFA
                     LVGVLLAAGSMNLTDIVNSQKGGMLHWWFIPLLPLFLVFWISGIAETNRAPFDLAEGE
                     SEIVAGFHVEYSGIGFALFFLSEYASMILISTVLAILFMGGWLSPFEGITFLDQIFFI
                     VPGFVWLLLKISFFLFVYLWVRATFPRYRYDQLMRLGWKVLIPVTIVWLIVTALMVVA
                     HVKPWF"
     gene            26501..27001
                     /gene="nuoI"
                     /locus_tag="I9760_000812"
     CDS             26501..27001
                     /gene="nuoI"
                     /locus_tag="I9760_000812"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAT5042598.1"
                     /translation="MKKVYHYIIHYVRTYLLLELLAGLWLTVKYFFRKKITVQFPEEQ
                     TPLSPRFRGLLALRRYPNGEERCIACKLCEAVCPALAITIESEQRDDGSRRTTRYDID
                     MFKCINCGLCEESCPVDSIVVTPIHHYHISERGQNIMTKEKLLAVGDLMESQLAADRA
                     ADEKYR"
     gene            27013..>27275
                     /locus_tag="I9760_000813"
     CDS             27013..>27275
                     /locus_tag="I9760_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAT5042599.1"
                     /translation="MHEWVIQAIFYVFALLTVLSAVMVISQNNPVRCVLFLVLTFFTS
                     AVLWVLAEAEFLALILILVYVGAVMTLFLFVVMMLNIDIESMK"
BASE COUNT         7951 a         4616 c         5967 g         8741 t
ORIGIN      
        1 aaatatgtac ttacaatcaa tctttcccta atcattaccg ggttaaggtt ctcaggttct
       61 gtgagttaat ttgcttggag tataaagtgg ggcttgaaaa aaaaattaag ctaagccagc
      121 gttctctgga attattaaac gattacttaa atttgggtgg gtctgggcct tccaatttag
      181 tgaatgggag ttcgtcgatt gatttttcat ccttaactca actacttgcc agtaatcttt
      241 ccaaatcatc caaaacttca aaagaaataa aaaagaaaag agaatttata gaggcagctt
      301 tgtatgctga gttgttacgt gacttaagta cttcattgaa tggatcggat gaggaaatca
      361 agcctgcaga aaaagaacct cctgtctcta ataagttgaa attctggctt ttggcaatag
      421 ctgggacatt gttagccgca tgcgaggggt ttgacagcat tacaaccatg atggctgtat
      481 taccttttcc tgcctgggtt gtcctttgtg caggtatttt attttccgct ttatctgtcg
      541 ttgtatttta tgggtttgat ttagtccagg tctctcaaaa cctggaaata aaattaactg
      601 acactcccaa gctattggat ttatacttgt cacaaatgga agaaattaag gcgattagaa
      661 aaaaaattga caattattct cttgcctcta tgtcagcgga tgagttgaat gagttgcatt
      721 taatccttgt tatgctgcaa gaccgtgtca actctttagt gaaagcaggc aaaaaatttg
      781 atttggcact gaatagcccc aaaatggatg ttgctaaaac aattgtttct ggagtatctg
      841 gaatactctt ttttggtgga ggtttttttg ccgggcaatc agtagcgaca ttcatgctgg
      901 ctctggttat gacttctgtt acacctacat tttggccagt tttattattt agtgtgattg
      961 tcggcttggc tgcctttagt ttgtactggt atgtgcaacg cgtaggagta aataaactgg
     1021 ttagcgaatg gtttggtttg gacgaggata aaatagaaaa attatgtgga aagtccaaga
     1081 tggacaaaga attgaaaaag ctaaacaacc ttgatgaaaa agtaatgact acttcggatt
     1141 tgctctcgaa agtggctaat ttggagagta aactaataga tatcgggaaa caaatgaagg
     1201 agatgagtac gcaccaggct cctgaaaaga aattagaggg tgctactgga ttaaaggcaa
     1261 gttctaatgt gtattccttt cactttaatt cctcaaatcg ggatcagggg agcgtttacg
     1321 gtgagaggat ccttgatgat gctgtttctg attatggtag gggtcagaat cataatgatt
     1381 caatccatag cttatgttag ccaaatgcaa aggcatatag attaacaggg agtttccttg
     1441 caaataatcc ggattggctg acatataaaa ctcttttaat tcactgctct cttcattaat
     1501 tcttgagagc tgacttctta aaggaaccag tggggaattg acaaccatag gatgattatt
     1561 ggttgaaatc cagccgtttt gttttttaag ggcgtttaga acggtcaata tttgatctgg
     1621 aaccctttgt aatggtttag gatatattag gtcactcaat tgataaataa attcgtaagt
     1681 taacgggttg ttggcaaatg ctatgtaaac cagttcctct tgaggattgg ctaacttgta
     1741 ttttattgcc tgagttaggc cggcactttt aatagatgcg tgaatttggt attcaggatt
     1801 tagatacaaa taagcgatat aacaagtcac ttgttttttt cccagatcaa cagattgtct
     1861 gcagttacgg ctgaatccgg ctatttcttt attttctcca tagagaaccg ttaactcgcc
     1921 atacaaatca ggctgtatga tgaaacgtat aaattcttct tggctggcca ggtgataata
     1981 cttggagagg aagcgatata atttatttat taattttaag gaaatttttt tcttttcttc
     2041 atctacaaca taagagtgga tcgctttaac atcgtgtctg ttcattgctc tctccatgaa
     2101 acattttttt cgctttaatg gctcgctgta taatttatcc ataaattatc taagtaatag
     2161 cgaatagcag ataaaaaatt gtgcaactct ttaaatatag actcgttttc atttttgtca
     2221 aattgaagaa taacaattgt cgtaactcca tgaaacggtc atctcagtaa ctctgtggat
     2281 aggcaatata tggggaatga taaaagaatt acggtggttc ctcatcaagg atcttaaata
     2341 aatttataag ggagagtcaa taacctctta tattttcaaa atatttttaa caaaaggtat
     2401 ggtgatttta cgatgagcct cccaagatgc atcatctagc tggttgagga tctcatataa
     2461 atcatgcatg tttcgagagc aacggtttaa taaaaaatgt ccgacactct caggaagttc
     2521 aaatccgcgt tttgctgcgc gcattttgag tgtgtttatt ttttcgtcat cgtttaattc
     2581 attcaactga ataactaatc cccaacttag tctggagcgt aaatccgcaa gtttaatgtc
     2641 acattttatt ggtggttgat tacctgatat aattaaaaga gaacgttcgg agtccttaat
     2701 cttattatat aaatggaata aagcttcttc ccaggccgaa tcgttagcga tggtattaat
     2761 atcatcaata caaattaaag tttgatcttc taatccttca atgctttgtg gtccccattc
     2821 ttttaaaaaa gctaaaggaa ggtaaatggc tgattgggtt aaattaatgg cttggcaaca
     2881 agcttgcagt aaatgtgatt tgccacttcc tttagggccc caaagataga gcaacctgtc
     2941 tgccttaaaa gaaagcattt caattaattg ctgctgcaac aaggtattat taccccaatt
     3001 gaagtcgtcc agggttgctt catcattcaa ttttattgcc agggccagct gtttattcat
     3061 ttttacgatt ctttggggga ccaggctttt ctgctccagg tccaaacata atcaaggtag
     3121 gaagtaacgg tggtaaaggc agttaaatag atgagtatat caactaaatc ataggggaat
     3181 ctaaagtaag ctaattcaaa taaacataaa gtgactaaag ttaactggaa gccggtattg
     3241 agtttactga gtcgagaggg ctcaaaatcc agtttgcgct gtataaagta ataccaggct
     3301 aatactccca ttgaaatagt gaagtctctt aaaaaaacaa gaagcactaa ccaccagggc
     3361 aaggagttta agagagctaa agaaatgaag ctggaggcaa ctaacaattt atctgcaaga
     3421 gggtcaacaa atgagccaaa aaaactttgc cattgaaaat gcctggctaa ccaaccatcc
     3481 aaaccatcag ttaatcctgc ggcgataaat atataaaagg ccttaacgta ttcatgatga
     3541 aataaataca ttagaaaagg agctattaaa actaatctta gcaatgttaa ggtattggga
     3601 atatgtttta gtatcatctg ttatacgaaa attagttcct cctctttttt tatttatcgc
     3661 tgaggaggga atatattgtg ccaacaatac tctattattt gagtgtttca aatggcgtat
     3721 gtgaattagt ttatacaata ccattgtctc tgtcacctta ttgagaaatc aattgggatt
     3781 tattgactca tagtatccac atcccggcct cgagaagttc tcatatttta agattggaac
     3841 aagagcgcat ggggtttttc gtcagtccag tagagcttag atctctgata atgggttgta
     3901 ttcctttatt gtaatatttc gattgatttt ggaatgcaga cttaataaca attcaggttg
     3961 atgctcccag ttatcatgaa gcaatagatg ttgttgccaa cttagaatac gcggatcaat
     4021 gtctttggat aagtctttaa atacagtttg catgacttgg cgcgttaatc cccataaggt
     4081 tttttcagaa atctgatact cattacgtaa acagccaatc aagtgatgta gatgattttt
     4141 taatgttccc tggatgaaaa gggttctaac ctgattaaga tcttttgtat agatggatag
     4201 agagtctgga gcgttgggtt tttgaacgtt tttaaacaat tcatgattgc atagcttaag
     4261 gttgtttggc tcttttataa ttatcccctg aggtttattg tcatcgaaga tgactaaaat
     4321 attgtgttgc tccacttcta gtgctaatcc atattttagc aatagatgca attgtccaaa
     4381 taacatctta taacaatatt cagtgaaata agtcgttggg tgtagttcgc tagctttaat
     4441 aatttcaatg agtaatggtg tattgctaca aggtgaatca gttaataggg caggtaatgg
     4501 aactgctctt tgatcctgtc ttattttgtg gagtgggttt tgatataagc caaacaataa
     4561 ttttactcga ttgcattcag agagagtttg atcataggtg ctcatgctct ccaggttttt
     4621 acatataaac aaggtatttt ggtaatagtt tgtttttgct aacagggaat cgatccatct
     4681 gctaagcaat accatgtcat cagaattatc gagttttcca gccattgagg tgcttaaagt
     4741 ggtagccagt ttaagtgtac attgagtgga ttgtgttggc atcataatat caggtgataa
     4801 agaaggtatt agtgtttggt gatgaggtaa caagatgagg gatttattat caattaaaga
     4861 tgcaaacgcc atttgtaatt gattcctcca ctgccaaggg tgaacaggaa ttggataata
     4921 atcatcaggg ttgatgtggc taaataacaa tttttcacgc caataaaaat attctttggg
     4981 aaattcttga gaaatttggt tggcataatc aatttttgag cttattgact gaatttttgt
     5041 tttatttaat gcacaccaat gtacactgac tttggcctgg aattcgggag agttttggag
     5101 tacttctctt cgcgtaaacc ctgtttttgc gctgaatgcc gggtgatagg gatgcctcaa
     5161 actgccccac tgttccagaa ataataacgt ttgatacgga gatagttcat tatttatcca
     5221 tgtccacaga ctcttgtagt gcatggcttc atttcttaat tgggtgttcc agttttgcct
     5281 gtaggcataa gccaaagcct cattagcaat cgattcatct aatgcttccc tgatttttcg
     5341 ccattgaaaa aacttgcttt ccggcattga atttttcagt tttatctgca attggtcaat
     5401 gaaatcatgg atatggtgac ttgcaatggc tgattgaatt aatccttcct taatggaggc
     5461 atgctgtaaa cgcttcaggg tatttttatg ggctaaggta ataaaataat ccacactatt
     5521 ttgtggtagt cctatgccaa tttcaaatag taaaaagcgt aattgatggc tgagttcatg
     5581 aaaattaccg tacgccaggg ccattattat ctcctgaagg attattttgt tataaattaa
     5641 atataaatga taatcattct catttgcaag gggtagggca aataaaatat tattaattga
     5701 caatagagtt tgattttgga taaaaagttt ctgtattctg agttaaatga taatattttc
     5761 tacttttatt atgtaaatga gtaagtggca tgaattatag acccgatata gatggattaa
     5821 gggccatcgc aattttattg gtcttaattt atcatggggg cctctcgctt tttccatcag
     5881 ggtttatcgg tgttgatgta ttttttgtta tatcaggttt tctcattact actattatcc
     5941 atgagtcact gaataagaat gatttttcat tcttcgattt ttataatcga aggttatggc
     6001 ggttacagcc tgttttcgtt gctctgatcg tggtgacaac cctactaagt gtattgtttt
     6061 atttgcctga tgatttaatt gaatttagtc gtagtgcgcg taaagcatcg ctgtttattt
     6121 ccaacctgtt ttttaataaa acaaccaccg gatatttttc acccgatacc catcaacttc
     6181 ctttgctgca tacctggtct ctttcaattg agtggcaatg ttatctggtt ttgccactgg
     6241 cgatgtatgg cttatatcgg tttctaaata aagggcaggt tatctatatt gtgcatgggt
     6301 tggccatttt attttttttg ctggctttgt actactcaca ggcggctcct gcccaaacct
     6361 attatcaatt ttccagtcgt atttttgagt ttttaatcgg ttcttgcatc gctttaagcc
     6421 ctcgaattga gttctcaatc aataagtcaa ttttagttat tttaggctta tccgcattat
     6481 tttttataat ttataccgct acactggata atattcttct tggatatcct aattggcgta
     6541 cactttttgt gtgtctggca acgggtttgt tgatagcatt aggtacaaat tttccttcac
     6601 ttgctattgt aaatgctctc tcttttcgac cattagtgtt tataggagtt ttatcttatt
     6661 ctctttatat ttggcattgg gttgtctttt caattttaag gtaccaaaat atcgaagagc
     6721 ggtctgaggt tttatttata gtttatggca ttgtttttgt tttagcttat ttatcctggc
     6781 gatatataga aaaaccgacg aaaaaattca gacatattcc ttttcgttat acggttgtga
     6841 ttttattgct gatccctgtt ttattttctc atttaagctc tttagttatt aaatctcatt
     6901 ctggatatcc acaacgattt aatcaggagc tggttagggt ttataaacaa ttaaagagtt
     6961 acgaaagccc atacagagcg ttatgtatag ataataccag ggcagacaat gcgtggaaat
     7021 gcaaaatagg agcaaaagag caaggtggca ggactggcct tatgataggt gattcttttt
     7081 ccaatcatta ttgggggttt atggatgtgt tagggaaagc tgccggtgtt tcaatattgg
     7141 ctcaaggaac ctcttcttgt atttccttac caggcatata tctttatgat tggtggcatt
     7201 tcaaagataa aatatatcag gattgttatg agcaaacgga aaaatattat cgcatgattc
     7261 aaaagaatca ttacgattat gtaattatag gtcaagtatg ggaaaattat gtttcagccc
     7321 atattatcaa tcaacgtggc gatgattatt ctgcagaatt aaccaaaaag cgtttggcag
     7381 atgccttaga cagagcactt ggcgtgataa tatcttctgg agcaaaacct gtattaatcg
     7441 aatcaaccgc ggtaacgcat ggaaatttac atcaatgctt ttttaagcat attaaattaa
     7501 gacaaagtta taactcagaa gagtgccgct ttactttaac cagttcagat aatgagactt
     7561 ggctaaacca attgtttgat aacatgcgaa gaaaataccc tcaattgata ataatcgatc
     7621 ctaaacaagt tcaatgtcgc gataatgttt gctattccga tttgaacggg attccagtat
     7681 atcgtgatga agggcatatt actgattacg cttcatacca actgggttat ttatatttgc
     7741 gggagtttga aaatcctttg atttaaaaag gttggtttgc tttgattccc cagactacaa
     7801 aacgatttta caaattttat tcttcatttt tacgatacag aaccagtgta tttcctatca
     7861 tctgaactaa ttcggcatgc aattgttcac aaagctcctc agccatttgc attctgtctt
     7921 ccttttcagc cccattaatt ttgactttaa ttaattcatg tgctaacaag gctacattcg
     7981 tttctgccaa tatagcttca gttaagcctt tggcgccaag gagcacaacg ggctttaaat
     8041 gatgagcctt ggctttaagt gattgtttaa aagacgtatc cactgcgata tcccagataa
     8101 taaaatggtg aattattgca cgttttgctg ggaaaaggta gtaaaattct gtatcatagg
     8161 aaaaattgtt ttatctttat tcgttatgaa tcgtactaaa agcagtaaac gttggctgca
     8221 agagcatttt gatgacgttt atgtaaaaaa agcgcaggct gaaggatatc gaagtcgagc
     8281 cgtatataaa ctcaaagaaa tagatgacaa agagtcactc attaaaccag gcatgacagt
     8341 ggttgattta ggggctgctc ctggtggctg gacacaatac gcctcagaaa agatgagagg
     8401 aagcggtcgt cttgttgcat tagatatttt acccatggat gctttgccta atgtggagtt
     8461 tatccttggg gattttcgtg aagataatgt cttgcaagaa ttaattaatt tgattcctca
     8521 aaggacttta gacttgttat tatcggatat ggccccaaat atgagtggaa gctcagcaat
     8581 agatattcca agagccatgt atctggtaga gctggctttt gatttcgcgg aaaaaatgtt
     8641 aaaacccggt ggtaatatgc tagttaaaat ttttcatggc tcgggttttg atgaattagt
     8701 caaacaggca cgagcgtctt ttgaaaaagt ggtaatccgt aagccatccg cttcaagatc
     8761 tcgttcaaaa gaaacctatt tgctggcaaa gggctataat ttatagtagt tttatatata
     8821 tttaaaatat gcttggcgtt gttttggatg atatgagctg tgcaagagct attttgggtc
     8881 tcgcaacaaa attgtaagta actaacgagg ttaatacatt gaacgacatg gttaaaaatt
     8941 tatttttgtg gctgataata gcgatagttc ttgtctctgt tttcagtaat tttggtcctc
     9001 gtaactcagt tgctgagaaa atttcttata gccagttttt acaggaagtg gatcaaggga
     9061 tgattaactc tgttacgatt gaggataaca aaatcatcaa gggcgtaacc aagaataata
     9121 aacgctttgt cacgtacatg ccgatgcaag acaatgcttt gttaggcgaa ttgttgaaaa
     9181 gcaaagtcga tgtcagtggt caggaaaaac aacaggaaag tttccttttg catttattta
     9241 ttaactggtt cccaatgttg ttattgatag gtgtatggat atttttcatg cgccaaatgc
     9301 aaggcggcgg tggtcgcggc gcaatgtctt tcggcagatc aagagctcgg ctattaggtg
     9361 aagatcaggt taaagtcacc tttgcagatg ttgcaggtgt tgatgaagcc aaagaagaag
     9421 tgaaagagct tgtcgatttt ttacgtgatc caacaaagtt tcaaaatttg ggtggacgca
     9481 ttccccgcgg tgttttgctg gtcggttccc ctggtacagg taaaacatta ttagccaggg
     9541 cagttgccgg ggaagcgaaa gtccctttct ttaccatttc aggttctgat tttgttgaga
     9601 tgtttgttgg ggtcggtgcg tcccgagtgc gtgatatgtt tgagcaagcc aaaaagcatg
     9661 cgccatgtat catctttata gatgaaatcg atgcagtagg tagacacaga ggcgccggct
     9721 tgggtggcgg tcatgatgaa cgcgagcaaa cacttaacca attgcttgtt gaaatggatg
     9781 gatttgaagg taatgaagga gttattgtcg tagcggcaac taaccgtccc gatgttttgg
     9841 accccgcgct tttacgtccc ggccgctttg atcgtcaagt cgttgttcct ttgcctgata
     9901 tcagaggtcg tgagcaaata ttaaaagttc atttacaaaa ggtccctgta gatagccatg
     9961 tagaagtgaa agctattgca cgcggtaccc caggtttttc aggagcggat ttagccaatc
    10021 ttgttaatga agcggcatta tttgctgcca gggctaataa acgcaaagtg ggtatgattg
    10081 aactggataa agcaaaagat aaaattatga tgggggctga aagacgctcc atggtaatgg
    10141 atgataacga gaaaaaactc accgcatatc atgaagcagg tcatgctatc gttggtctgt
    10201 cagttcctga gcatgatcca gtttataaag tatcaataat tcctcgaggc cgagctttgg
    10261 gtgtaactat gtttttacca gaacaagaca gatatagtca cagcaaacgc cgtttggaaa
    10321 gtcaattatg cagtctgttt ggtggtcgaa ttgcagagga attaattttt ggtcctgaaa
    10381 gtgtgacaac gggtgcttcc aatgacatta tgcggtctac tgagattgct cgcaaaatgg
    10441 ttacaacttg gggcttatct gctttaggtc ctctaacttt tggtgaggaa gaggaagaaa
    10501 tatttttagg acgctcagtg aataaacata aggagatgtc cgatagaaca gcacagcaaa
    10561 ttgatgacga ggttcgtgct attattgaca ggaactatca aagagcaaaa gaaatattgg
    10621 aaactaatat cgataaatta catttaatgg ctcaaagcct catcaaatac gaaacgatag
    10681 acaccaatca aattcaagaa attatgtctg gtaaagagcc tacccctccg gaggattggg
    10741 ggtcaacaaa accgatggat aaggctgagt cggttaatga tgctcctgca aagcctatta
    10801 atggcgaaac aatagaaaat ccggcagaag gacattaatt ttatcagtag ttatttatcg
    10861 agagtaccct gtgaattctg aacagttcct cggttggctt gagcgaaaga gccaacctcc
    10921 ttcctccttg gtttttgaaa agccattaat tatgggtgtg ttaaatgtta cctcggactc
    10981 attttatgat ggtggcaagt atctgtctgt tgatagcgcc tgtgatcagg ccttaaaatt
    11041 aattgcatgt ggagctgatc tcattgatat tggtggggaa tcaaccaaac ctggtgctcc
    11101 cccagtccct ttagatgttg aactatcaag agttcttccc gtaatcaaac aacttcgtgt
    11161 cctcgcagat gtgtgtattt ctattgatac taataagcca gaggtgatgg aagcggcaat
    11221 cgatgctggt gcgaatgtta taaatgatgt ttatgcttta agaagggatg gtgctctgaa
    11281 tgttgctgcg cagttagatg ttcctgtttg tttaatgcac atgaaaggag agccgcgaac
    11341 tatgcaagaa aatccttctt acccagaagg aataatgaag gaattacagc atttttttaa
    11401 agagcgcatc gataaatgcc agaaagctgg gctgaataag aataaattaa ttcttgaccc
    11461 tggctttggt tttggaaagc gcgttaaaga taacctaaaa ttaatttatc atctgaacga
    11521 attgcgttct tttggattgc caattctttt gggcgtttca cgcaaaagca caattggtgc
    11581 tgtattgaat aatgaagttg agcaacgttt gattggaagt attacgctag gaatatatgc
    11641 tgctcttaaa ggcactgcaa ttatcaggac acatgatgtg gatgaaacga atcaagcatt
    11701 aactatgatt aatgcaattt atcaagctgc taaagaaatc ggttaatagc tcatgaaatg
    11761 atcagaagac aactgagatt agaaaaatta aataataagg taggtaagga tgagtcaacg
    11821 taaatatttt ggtacggatg gaatacgggg tcacgttgga ttatcaaata ttaaccctga
    11881 atttgtttta aaattaggct gggcagtagg ctgcgttctt gcgaacggag cgaggaaaaa
    11941 ggtagtgatt ggtaaggaca ccagagtgtc tggctatatg ttggagtcgg ctttggaagc
    12001 aggtttgtcc gcagctggag ttgatgtggc attattagga ccaatgccta ctcctggaat
    12061 agcctatcta acacaaacat tgagggctaa tgcaggcatc gtgattagcg cttctcataa
    12121 tttatttgaa gacaatggca ttaaattttt ttctgctgat ggaggaaaac ttccggatag
    12181 tgtagagctt gcaattgaag cccaattaga aaaacagttg caaactgtac cttcagccaa
    12241 gctgggtaaa gcaacacgta ttaatgatgc agcgggtcgt tacatagagt tttgtaaatc
    12301 tacgattcct tccctatctc gactatccaa tttgaaaata gttgtggatt gcgccaacgg
    12361 ggcaacctat catatcgccc caaatgtatt ctctgaattg ggtgctgatg tagttcctat
    12421 aggaattaaa ccagatggtt ttaatatcaa tcaagaatgt ggttcgacag cgcctgaatt
    12481 attaagagaa aaggtgattg ctgttggggc tgatattggt attggtttgg atggagacgg
    12541 ggatcgagtt attttagtgg attcactggg aaatctcgta gatggcgatc aaattattta
    12601 tatcattgcc aaggacagac accaacgagg agtactgcat ggaggggtcg taggcacgtt
    12661 gatgagtaat tatggtcttg aattagcaat tacctcattg ggggttcctt ttcaacgctc
    12721 aaaagtaggt gacaggtatg ttcttgaaac gctaagagag aaagattgga agattggagg
    12781 tgaaacttct ggacatattg tttgtttgga taaaacaaca acaggtgatg gaattgtagc
    12841 agcactgcaa gttctttcaa tcatggtgaa gcaaaataaa gcactgcatg agttaactgc
    12901 aggtattcaa ttgttacctc aaactttagt gaatttaaaa acgaataatg ctgccttgtt
    12961 agcctctaat cctgatgtaa ttcaggcggt taaaaatttg gaaaaacatc ttaatggaga
    13021 gggtagagtg ttactcagac cttctggcac tgagccttta ctgagagtta tggtggaagg
    13081 tgctaatgca tcgatagtca agcaacaagc acaaatgtta tgtgatgaca tcagtcagat
    13141 cgataaaaaa atgactgaga gcctaccgtc cacttgagca tctgtctttt taagttctaa
    13201 tgcaggggct ttttcaaaag cccctctgtt tcacagtata aggtattaac tacttgttgg
    13261 agccatggag tggagtattg tgtcaacctc ttcaagtaat cggtgagatc ttgttttgaa
    13321 aaaacaaggc ctttttgaat actcatattc atcttgccct gttaacttac ctattaacat
    13381 tcggaaaaaa tcttcaatat attcccatgc tcttataaat cctctatgcc gttttaaagg
    13441 ttcatatcgg ggatcttgag taatgagagg catggcttct cggtttgaag tttttgcatc
    13501 agggagttcc atcaaatgtt taattgaaat aagtgaatgg attgcctgaa taaataattc
    13561 agcttccaat ggatgttcct ttttcaattt tacatattgc tgatgaaatt tttgcaattc
    13621 ttcaggccaa ttctttaatt gctgttcttc atcaatcgat tcatcaacta taggcagagc
    13681 aggttttaag cggagaatat tttgtatatc ttgcaatggg tttgggcaca tgcgcttgta
    13741 agctagtaat acagataaat cattttgatt ttttaattca aacaagatgt cttttaaaag
    13801 atgaagtgct ttaagaccca atgcctcatt tacacaagaa ctttgcgctg catcatataa
    13861 acctttatac catagctgta tttcttctga atgttgcttt atatattcca tgttttgtac
    13921 atgaaatgaa tctaattttt gcttaagcag gatagaagat ggtggtaaag gggagatgct
    13981 aggggcagat tcctggtgtt gaggatttga taatttatct aactcatggc ctgtagaaac
    14041 aggcttttct ttaaatgctt tatcaatccc tgaggaaatt acttttcccc tgtctacggt
    14101 aaacgtctca gaatcttctt cagatgagat gctaatttga ttaccagcct catcaaaaaa
    14161 ggctaaaata ttatcttctg ataattcatc gtgaccatta aataatgaca agccatttgg
    14221 tttcctggat atagctggaa cttcaggggt tgatggtctc aatggttccg atgggcttga
    14281 tttgacaaca gccagtgttt cttgtccgtt gggtacttgt tgttctaaac tggactcaga
    14341 gccttgccga accacttggg tgtggggtgt taattccgta acgacagata tttcttgttc
    14401 tatctcttta tgttgctttt cgccagaaag cccagtgtct ttctgaacca taggagagag
    14461 tcgtgttgat tcaaggacga ttggtgtttc ctgtctctca gttacttgct gtttttctac
    14521 agtagccacg gaatcctttt caaccataga agagggtcgt attaattcca gtgtgactgg
    14581 tatctcttgc tctacttcta catgctgttt tttaccaaca gattcagact cagtatgaat
    14641 gatttgagga gagcgtgttg actcgagaac aaccggtatt tcgtgtccat tgtctacttg
    14701 cagtttttca gtggtagatt caagttcctt atgaatggct tgaggagtgc atgttgaatc
    14761 cagaacaacc ggcatttctt gtccattgtc tacttgcagt ttttgggtga tagattcaga
    14821 ttccttatga atgactggac gggggcgtaa tgattccaga acaaccggcg tttcttcctc
    14881 attgtctagc tgctgttttt ctatgggaga atcattgcct atctgaatta cttgggcagg
    14941 gtgcatcaat tccacagtaa gagggattgt tttatctact gctatttcct gtttttccaa
    15001 tggaggatca attttttcac cggacagatt aggcaaaggt aattgattta attcagacac
    15061 aagggattgg atttgattgg caagagtaga taaatcttct ttaatagagt ttatgtcttc
    15121 gttctttagt ggttgatttg aaattaaacg taattgctca ataagtgctt ttgcttcatt
    15181 ggttgtttgt ccatgatctt tcataagcaa atcaatttgc ttggctgttt caagataaga
    15241 ttggcatagt ttaatctggc tttttaactc ttctttaagt ggatcctctt cattcaatga
    15301 atctttactt tcttcagctt cttgcaaact tggaagttgt acatccatag ttataggtaa
    15361 atcgtatcca cttcttagtt gttgtatttg tttgcccaga gcaggcattt cttgaagttt
    15421 ttccaacgct tgtgaaaatt gttccttctt ttccgaaact tttggcatga gaatgagaag
    15481 taccaaataa aaatcggccg gcgaagagtc ataagaaagg ccacttggta aaacaaaatt
    15541 tggtatactt tctattggta tttcatttat caggtcaatg gcatcaggaa ctaatttttt
    15601 ataaatagat atatcctctt catttgaagg agtttctggt atcgctttaa tcaacagttc
    15661 ttgctgtttc acaagttcat gatgtttgat ttttagctct ttttgttgag ctaataattg
    15721 caggaagcta actgaattct taagtttttg ctgattatca gaataattag aatccgaata
    15781 ccatgaaagc caggaagatg ggttaaaatt agataataat ccatcaatct ggttttggta
    15841 ttcctgtatc aatccttgag tgtcttcact ttcttgaaat ttcctggtta actctgcttt
    15901 taaatcgggt gccagggagt ggttagttac tacggtttca atacgaccca ggtctttaag
    15961 caatagttca atttgttttt ctagctcagt ctctttttta ttaatatcag ttgcagataa
    16021 ttgtttggcg aatcgctttt gcaattcatc ccatttttca caagcgcgag ttaatttttt
    16081 aatttccaat tcaactgcag ctataaattt ttgtaaacta tcaacagcat cttcttccac
    16141 agggggttga atgccgaact gagacaaatc tgccattaag gcattccatt tatttttcag
    16201 caaaaccagc tttaatttgg cacgtgtagt ttctatttcc ttactggttt tgaagagata
    16261 actgtagtgc ccaaccacga ttgatttttg aacctctccg tattcaggga atttataagg
    16321 aggcgctaat ggattttgtt tggacaggac ttcgttcgct ttaggctcga tttctttgta
    16381 taaaggattt aattcatttt ttgcgtgctc aattttttta gccaaatcag aaatttttct
    16441 ttgcagttct tctccttttt tcatgacctc atctagtttg tgtttttttc ttttatactc
    16501 ttcttgttct tcagctaaac tggaatattc atcaacatat tttttaatta acaataaaag
    16561 atttaactgg gattcaactt caacaggagc ttttccttcc ggcagtaggg ttgaactaaa
    16621 tatttgcttt aaatttaagg ggttaattgg tggagtcaag gtagagattt cattcattaa
    16681 gtgcagtatg atttcgttca ttataaaacc ctcctggtta aatcatagta ggattctatc
    16741 gaaatcaaac cactaagcaa gtagaaaaat gtattttttg caaaaaaaat gaataattaa
    16801 ttctgaaaaa aaacatagat aaagctacac atctgagtga agtttcggta tattgcacag
    16861 ctgcacacta tcagttgttt actgataggc tggaaataat agaaaggatt ttgtccgcct
    16921 atactagaaa ttattggagg gagtgtgagg caaaagatag ttgctggtaa ctggaaaatg
    16981 aatggccaga ttcaacaggt gactgaattg gtttcgcaaa ttgaggaatt aattggcttt
    17041 gattgcgcag cacaggttgc tgtaatgcct cccagcattt atatacccaa ggtaagagac
    17101 tgcttaagga cggggaagat tgtagtaggt gcgcaaaatg tttatccgaa agattatggt
    17161 gcctacacag gtgaactgtc agctcccatg cttaaagatt ttgattgccg atatgttctg
    17221 gtaggacact cggagcgcag gcaatttttt catgaagatg aaaattttgt ggcgcaaaaa
    17281 ttccaccatg tcaaagatca tggtatgata cctgttcttt gtgttggtga aactctttct
    17341 gaaagagaga atggaaaaac agagcaggtt attgctcaac aggtgctcgc agtgagtgca
    17401 aaagggaaag attgttttcg tgattgcgta gtggcttatg agcctgtatg ggcaattggg
    17461 acaggaaaaa ctgctacacc tgaacaggca caaaaaatac accagtttat tagagatctg
    17521 gttggagaaa taaatgatag cgatgcgaaa catttgacgc tcatatatgg tggcagtgtt
    17581 aatgaaaata atgcaaaagc cttattttcc atgccagata ttgatggagg gttagtgggt
    17641 ggagcatcgt tgaatgcaaa acaatttgtg gaaattgtga aatgtatcaa ttgatattaa
    17701 tgattcatgt cttaattgct gtaattttaa taggcttggt tcttatccaa catggcaagg
    17761 gtgctgatat aggtgctgct tttggttctg gcgcatcaaa cacgttattt ggaagtcaag
    17821 gtactggtgg cttcttgttc aagttaactg gtggtctggc aataactttt tttattacca
    17881 gtttgatgct gtcttatatg gtttctacgc agaaccaaaa ggcaggacag ttagctattc
    17941 ctcaacaaac cggtgctcca gtcagttcag tcccagtgcc agttgaaaaa aataataaga
    18001 agtaactaaa aagatgtagt gtctttaggg ctgttgtctt ttgtgatgaa ctcaggataa
    18061 caaaaataaa aataagccga agtggtgaaa ttggtagaca cgccgtcttg agggggcggt
    18121 ggcgcaagcc gtgccggttc gagtccggcc ttcggcacca ttttaaatcg agcaaggtga
    18181 actgatgcta tctcaatatc tacccgttct cgtttttata attgttgcct tgattttagg
    18241 tgcagtaatg ataggcgctg gttctttgct ttccaagcat agtccggata gcgctaaaaa
    18301 ctccccctat gagtgtggtt ttggcgcctt tgaaagctcc catatcccgt ttgatgtacg
    18361 gttttatctg gtggctattt tatttattat ttttgatctg gaaacagcat tcttttttcc
    18421 ttgggccttg gcattacgta aaataggatg gtttggcttt tatgctatga tgttatttct
    18481 tggactgttg gttataggtt ttatttatga atggaaaaga ggcgccctag agtgggaata
    18541 aggttctttc attcttttaa aatatttgat ttaatctttc attttagcca ttactatggg
    18601 aagcaaagga cgcttggcaa atcattacag agctttttgc ttttagatga acctgatgga
    18661 tttttaggtt ggtttttaaa acttggtcat cctttgccaa aacgatagct gagattatat
    18721 tttaagccat tgtaataatg gttatgtaaa aaactggatt attaattatt taactgctta
    18781 gaggcgaact atggcggttg cagaactaga aaaaaaaggt tttgagttaa ctacggtcga
    18841 aaaattggtt ggttgggcac gcagtggctc catgtggcca atgacatttg gcttagcttg
    18901 ctgtgccgtg gaaatgatgc acgtgggagc agcacgctat gatttggaca ggtttggtat
    18961 tattttccgc cccagtccac gccagtcaga tgtcatgatt gtagcaggga ctttatgtaa
    19021 caaaatggcc ccggcactca gaaaagttta tgatcagatg ccagaaccac gctgggttat
    19081 ttcgatgggg tcttgtgcca atggtggggg atattatcat tattcctatt cagtggttcg
    19141 tggctgcgat cgtattgtgc cagtcgatgt ctatgttcct ggctgtccac caacagcaga
    19201 agcgttgtta tatggcatta ttcaactaca gaataaaatc aggcgaaaac ctgttttaga
    19261 ggcataagct cattaagggt gcagattaga tgacaaaaaa tgagtatttg attgaaaagc
    19321 tacaagctga tttagcaaat catattaccg agttaacctc cgcatatggt gaggtaacta
    19381 ttgagtgtga ggtgcaaaat ttattgcctg ttatgattga gttgagagac agagaggaat
    19441 tttctttcga tcagttaatt gatctctgtg gcgtagatta tttgcattat ggtgattatg
    19501 attgggaaac agagtctgcg acagaacatg gattttcacg aggtgtcgag cgtcaggaag
    19561 ccaaggctta tgctgtaaat aaaccacgat tcgccgtagt ctatcattta ttatcaacca
    19621 aaaaaaatca tcgtttacga gtcaaattat ttgtagaaga atcccattta attgttccat
    19681 cagtacatca tttatggaag tctgctaact ggtttgagcg ggaagcttat gatttatatg
    19741 gaattttatt cgatggccat cctgatttaa gacggctact gacggattat ggttttatag
    19801 gccacccttt ccgcaaggat tttccactca gtggagaggt agaaatgaga tatgatgcaa
    19861 aactgcaaaa ggtaatatat gcccctgtag acattgtgcc aagaattgta gttccaaagg
    19921 ttattcgtaa cgataaccgc tacataggta atgaaggtag caaaaatgat tgaacttaaa
    19981 aattatacct tgaattttgg cccacaacat cctgctgcac atggtgtttt aagattggtt
    20041 cttgagttag agggtgaaac tatcgttcgt gctgatccac atattggatt gcttcatcgt
    20101 gcaacggaaa aattggctga gaccaagcct tacattcaaa gcattggtta catggacaga
    20161 ttagattacg tctcaatgat gtgcaatgag catgcttatg tcatggcgat tgagaaatta
    20221 ttgggtatag aaccgccttt gcgagccaag tatatacgaa ctatgtttga tgaggtgact
    20281 cgaattctga accatctctt gtggttagga gctactgcat tggatattgg tgcaatgaca
    20341 gtgtttcttt attgtttcag agagagagaa gatttgtttg attgctacga ggcagtttca
    20401 ggagcacgta tgcatgctac ctactatagg ccaggagggg tagcaagaga cttgccagat
    20461 actatgcctc aatacaaacc gtcaagatgg catagcgaaa gagagattga aaaaaagaat
    20521 cacaatcgtc agggcagtct tttggatttt ctttgggact ttactgaacg atttccccac
    20581 tgtgtggatg aatacgaaac attgttgacc gataatcgta tatggaagca acgtactgta
    20641 gatattggtg tcgtttcacc tgaaaatgcg ctgcaatggg ggtttaccgg accaatgtta
    20701 agaggctccg gtattgcctg ggatttacgc aagaaacaaa gctatgctgc ttacgacagg
    20761 gttgaatttg atattcctgt tggcaagaca ggggattgtt atgatcgata tttggtaaga
    20821 gtcgaggaat tgagacaatc caatcgaatt ataaggcaat gtattgaatg gttgcgcaag
    20881 catcctggtc cagtaaaagt cgatgattat aaagtgtcgc ctccacgccg tgtagtaatg
    20941 aaacatgaca tggaagcatt aattcatcac tttaaattat ttaccgaagg cttttgttta
    21001 ccgcgaggtg aagtttattc atctgttgaa gcgccaaagg gcgagtttgg gatctatatg
    21061 gtgtcagatg gagctaataa accttaccgt ctgaaaataa gagctccagg ctttgctcat
    21121 ttatcaagct ttgatgatat ggttagaggt cacatgctag ctgatggagt ggctatattg
    21181 gcaagtcaag atatagtgtt tggagaaatt gatcgttaat ttaacgacat gcttgcttaa
    21241 agtgtattaa gagataataa acgatataaa ggtttcgaaa tgtctgctaa ttcagcaaaa
    21301 atattaagtc aattgatgcc ggcaaaccgt atgctcgata ttgatcattg gatatcaaaa
    21361 tatcccaaag atcaaaaaca atccgcggtc atgagtgctc ttcgaatagt acaagaagaa
    21421 catggtcatt taacaacgga attaatgaat gcagtagctg aatatcttga tatgccgccc
    21481 atcgcagttt atgaagtagc aagtttttat acaatgtatg aacacaagcc agtggggcga
    21541 catttaatta atgtttgtac caatatttct tgcatgctga gggattctgc tggcgttgtt
    21601 gaacatttag aaaaaaaatt gggtgttaat ctaggtggaa caacagaaga tggtcgattt
    21661 actttaagat cagttgaatg cttgggtgct tgtgtgaatg cgccaatgat gcaagttgat
    21721 aaagattatc acgaaaattt gactgctgaa tctatagata aagtattgga acagtaccaa
    21781 tagaaaataa aggtgtttgc catggttgaa ctaaatcaag tttgttaccg aacatttcat
    21841 ttaccagagt cctggtcgct atcaacttat gaaagtattg gtggctatag cgcatggcga
    21901 aaaattttaa aagagcaaac atcacctcaa ttaataattg aagagttgaa aacttctgcc
    21961 ttgcgcggac gaggaggggc tggattccca accggtttaa aatggagttt tatgaataga
    22021 aactcccctg ttcaaaaata cgtagtatgt aattctgatg aaggtgaacc gggtacttgt
    22081 aaggacagag agattcttac tttaaatcct caccaattaa tcgaaggaat ggccatagct
    22141 gggtatgtta tgggcgccac ggttggttac aactacataa gaggagaatt ctggctacct
    22201 tttgagcgta ctgaggctgc aataaaagaa gcatacgatg ctggcttgct aggtaaaaat
    22261 atattaggct ccggctttga ttttgattta tataatcatt taggcgctgg tgcttacatt
    22321 tgcggtgaag aaactgcatt gcttaactca attgaaggaa aaaagggaat gcctcgtttt
    22381 aaaccaccat tcccggcaaa ttatggcttg tatggtaaac cgacaaccat aaataacacg
    22441 gaaacatttg cgtctgttcc ggttattatg gaaaaaggtg gagaatggtt tttaaaatta
    22501 ggtaaaccga acaatggcgg tgccaaatgc tttagtgtta gcggccatgt taacaaacct
    22561 ggtaactatg aaattccctt gggcacgcca ttcaaaacgc tacttgatct ggctggcggc
    22621 gtgattaaag gccgaaaaat caaagccgtg attcctggtg gttcctctat gccagtgctc
    22681 ccaggcgacg tcatgctggg gctggatatg gattatgaca gtatacagaa agccggctca
    22741 ggcttgggtt caggcgcagt cattatcatg gatgaaacca cctgcatggt ggatgctttg
    22801 tatcgtattt ctgagttcta catggatgaa tcctgtggac aatgcacacc ttgtcgggaa
    22861 ggcacaggtt ggttagtcag gttattacac agaattataa acggtcatgg cgagccggga
    22921 gacgtggaga aattagtgaa tgtggcgaat aacatagagg gaagaaccat ttgtgcatta
    22981 ggcgaggcag cagcttggcc agtacagagt tttgttaaac atttctatga tgaatttgaa
    23041 tatttcataa agcataaacg ctcgatcgtc gcagcataat taagaaggtt agaccatggc
    23101 tactattgaa atcgacggta aaacatttga agcagaaaac ggtaaaatga ttatagaggt
    23161 tgctgatgag gctggcatct atattccacg tttttgctat cataaaaaat tatctgtggc
    23221 agctaattgt cgcatgtgtt tggtcgaagt ggaaaatggg aggaaaccgg ttcctgcttg
    23281 tgctacacct ataactaatg ggatgaaagt atttacaaag tcagagcagg ctatacactc
    23341 ccaaaaggta gtaatggaat ttctcttgat aaatcatcct ttggattgcc ctatctgtga
    23401 tcagggtgga gagtgtgagc ttcaagatat ttctatgggg tttggcgctg ataaatcaga
    23461 gtacacagaa accaagcgtg cggtagacga tgagaattta ggaacactaa tatctactga
    23521 aatgacacgt tgcatccatt gtacacgctg cgtgcgcttc ggcgaggaaa ttgcaggggt
    23581 aagagagctg ggagctaccg gccgtggcga aaagacgcag attggtactt atgtagaaca
    23641 cagtatgaca tcggaagttt cagggaatat catagacttg tgccctgttg gcgccttaac
    23701 ttcgaagcct tatcggttta aagcccgtgc atgggaattg actcagcatg atagtgttgc
    23761 gcctcatgat tgcttggggt caaatgttca tattcataca agaaataata aattaatgcg
    23821 tgtagttccc agggaaaacg aatctatcaa cgaaacctgg ctttcagaca gagacagatt
    23881 tagctatttg gggttaaatg cggtttcaag ggcgagcaaa ccacaaatca aaaaaaatgg
    23941 ccaatgggaa gcggttgatt gggaaaccgc acttaagttt gccgcagaag ggataagccg
    24001 agttatcaaa caacatggcc cagagcaaat ggccgctttt gcgtccagtt cttctaccct
    24061 ggaagaaatg tatttactgc aaaaattaat gagagagcta ggtgttcaaa atctagatca
    24121 ccggttacaa caaattgatt ttagagatca ggcattttta cctactacgc cagtaagcac
    24181 tttgccttac gcagagatag atcatcagca ttcaattctg ttaataggat gcaatattca
    24241 tagagaagtg ccgttggcag gaacaagggt aagaaaagct tttcgcaatg gcgcaaaaat
    24301 ttatgccctt aatccagtag attttgatta tcattttgat ttatccggac gagtcattat
    24361 ttctcctttg gaaatgccga tgcaattagc taaattagcc ttagcgctta caaccgaatt
    24421 ggcatcgctt ccagaagaag ttcaaaaact gttaataggt ttggaagtag ataaacaaac
    24481 caaacaaata gcacaatcat taaaagaaga gaaagcctgt ttgataaccg gagcaatagt
    24541 tgaaaatcat ccagaagctt ccttattgcg tactttggtg gctattgttc aaaaacttag
    24601 tggagccaaa ttagtcagat taacgactgg agcaaacagc gcgggcgcat gcattgcagg
    24661 tatgctgccg catagaacag ttgctgggaa atcaattgca gagccaggtt tcaatgtaca
    24721 agaagcgtta aattccaagt taaaaggtta tttactcatg ggtgttgaac caggctatga
    24781 ttttgcaaat ccagccgggg caaggcaatc aatgttagct gcagagtttg ttgtactctt
    24841 atcagcctat gagcatgaat ccatgcatga ttatgcagat gttattctac cgatcgctcc
    24901 ttatgctgaa acatctggaa catacataaa cattgataat acctggcaaa cagtaaaagg
    24961 agccatgctg ccatttggcg aatcacggcc agcctggaag gtgcttcgtg ttttgggtaa
    25021 tttattgcac tgcaaaaaat ttgattatac ctcaacagag gacatcttgg aggaagtcaa
    25081 agaagcagta tcgatgacaa tggagcacga atacgaacct tactatcctg aatcattgcc
    25141 tgtcattaat caatcactgg taagagttgg agagtggcct ctttatcgaa tagatgctat
    25201 tactagaaat gcaaaagagt tacaattatg tgccgcatct gaatctgcct gtataagaat
    25261 tcatccatca acggcagata gattgaaatt agaagaaatt gctactgtat ctcaaggaga
    25321 tattgagata acattgccgc taaaacgtga tgagcgcatt gcggttgatg ttgtgtgggt
    25381 ggcaaatgca atgccagaaa cagttgacct tggtcactcg tttgctgcaa taacaataaa
    25441 gcgctagaaa ggtataaaaa tgcttcatac tattggcata ctaatttgga ttattattaa
    25501 aattcttgtg atagttgtcc ctttactgat ttccgtggcc tacctaacct atgcggagcg
    25561 taaagtgatt ggctatattc aagtgcgtat cggcccgaac agggttggtt taaagggctt
    25621 gctacagcca tttgctgatt tattgaaatt aataactaaa gaaatcatag ttcccactcg
    25681 atcaaataaa tacctttttg ttattgctcc actttttgct ttagtcccct ccttagtcgg
    25741 ttgggcggta atcccttttc aggaaggtat tgtcctggca aatattaacg caggggtctt
    25801 atacttattt gccatgagtt cgttgggcgt atatggtgtt cttattgcag gatgggcatc
    25861 gaattccaaa tatgccatgt ttggcgcact gcgtagtact gcccaaacag tctcttacga
    25921 aattgctatg ggatttgctc ttgttggtgt gctcttggcc gctggaagca tgaacttgac
    25981 tgatattgtt aattctcaaa aaggtggaat gctgcattgg tggtttattc ccttgctccc
    26041 cctattcttg gttttctgga tctccggtat tgcagaaact aacagagctc cttttgactt
    26101 ggcagaaggt gaatcagaaa ttgttgctgg cttccatgta gaatattctg gcattggatt
    26161 cgctttattc tttctctctg aatacgccag tatgattttg atttcaacag ttttggctat
    26221 tttattcatg ggcggatggc tgtcaccttt tgaaggcatt acgttcctgg atcaaatctt
    26281 ttttatagta cccggatttg tttggttgct tctgaaaatt agtttcttcc tttttgtgta
    26341 tttgtgggta agggctactt tcccccgata tcgttatgac cagcttatgc gtttaggctg
    26401 gaaggtactt atccctgtta ctatagtttg gttaatagta accgccctta tggtggttgc
    26461 tcatgtcaag ccatggtttt gacggttaga agagaagccg atgaaaaaag tatatcatta
    26521 tataattcac tatgttcgta cctacttact gttagagcta ttagccggtt tatggttaac
    26581 agtaaagtat tttttcagga aaaaaataac agttcagttt cctgaagaac aaacgccact
    26641 ttctccaaga ttcagggggc ttttggcatt acgtcgttat cccaatggtg aggagcgctg
    26701 cattgcatgt aaattgtgtg aagccgtttg ccctgcatta gctattacta ttgaatcaga
    26761 gcaaagagac gatggatcac gccgtactac tcggtatgat attgatatgt ttaaatgcat
    26821 taattgtgga ttatgtgaag aatcttgccc tgttgattcg atcgttgtca ctccaattca
    26881 tcactaccat atcagcgaac gtggtcaaaa tattatgaca aaagagaagc ttttagcagt
    26941 aggcgatttg atggaatcac aactcgcagc agacagagct gctgatgaaa aataccgttg
    27001 atggggtgat ttatgcatga atgggtaatc caagcaattt tttatgtctt tgcattattg
    27061 accgtactgt cggcagtaat ggtaataagc caaaacaatc cagttcgttg tgttttgttt
    27121 ttagtgctca ctttttttac cagtgcggtt ttatgggttt tagctgaagc tgaattttta
    27181 gcattaatcc ttatcctggt atatgttggc gctgtcatga ctttatttct gtttgtcgtc
    27241 atgatgttaa acattgacat tgaaagcatg aaaag
//
LOCUS       DACTJE010000025        25530 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.025, whole genome shotgun sequence.
ACCESSION   DACTJE010000025 DACTJE010000000
VERSION     DACTJE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25530
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.025"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            126..1763
                     /locus_tag="I9760_000814"
     CDS             126..1763
                     /locus_tag="I9760_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HAT5042600.1"
                     /translation="MKKISCSILLLINSLISYADSDQPKLILQIVVDQLRGDLIYRHH
                     KQFGPSGFNYLLAHGLDYHNAHHPHANTTTCAGHSTIATGSYPSLHGIVDNDWYDRKT
                     RKLKYCMEDLQAKILPTVHTHIEISGRSPRNLKPSTLSDEIILANKGKAFGVSLKDRA
                     AITLAGHAGKAFWFDKTNGGFITSTYYYSSYPLWVENWNKHYQPKAYDWNLSHPKSFY
                     QNANTLPFRHNYGSFGQTFPHHVTNPPSEEYFKSLSRTPKADELTADFAKQLLVNEKL
                     GKTTGQTDYLAISFSAVDAIGHQFGPNSLEAEDNLIALDDTLSQLFKTIDKEVGLNNT
                     LIILTADHGVSDGPSYLKAHKIPEIKPVNIEATGKHIENLLLKRFNLPAQALMSINPP
                     FIYLDHQIIKEKNLDIAEVSFYLAEEISHLPGIFKAYPLPIRDIEKDWLSAKVDRMSY
                     AYRAGDVYIVQPPYQSHGAKSEDRVAHGSPWQYDTYVPLLFVHPDFKPKRIFRQVHTT
                     DIAPTLSSLLLIKPPAAAVGQPLVEVVNAFHKTGEND"
     gene            1816..2454
                     /gene="udk"
                     /locus_tag="I9760_000815"
     CDS             1816..2454
                     /gene="udk"
                     /locus_tag="I9760_000815"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAT5042601.1"
                     /translation="MTKQAIIIGISGPSASGKSLLANTIVNELGSEQVVVISEDAYYK
                     DNGHLPFTEREKINYDHPDSFDHALLYEHLRQLRVGNTVQIPIYSHSKHLRLPETRAV
                     GQHAIIVLEGILLFSDKALREIMDIRIFMSTPLDVCLTRRLKRDVVERHRSFESVIHQ
                     YETTVRPMYMQFIEPSSRYADIIVPRGGENRIAIEMIQAKMRELLASHTRGS"
     gene            2568..3356
                     /locus_tag="I9760_000816"
     CDS             2568..3356
                     /locus_tag="I9760_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HAT5042602.1"
                     /translation="MGFLTGKKALIVGLASNRSIAYGIAKAFHNQGAELAFTYQNEKL
                     QSRVETMASEFNSTLVFPCDVASDEEIKAVFDNLRNHWDKLDILVHSVAYAPADQISG
                     DFVECANREGFRIAHDISAYSLIGLSQAALPMMLDTQGSILTLSYYGAEKAVPNYNVM
                     GVAKASLEASVRYLAASLGSRGLRINAISAGPIKTLAAAGIKDFRKIHAAYANITPLQ
                     RNVTADEVGNTAAFLCSDLASGITGEVLHVDAGYHAVSAMSELG"
     gene            complement(3894..5768)
                     /locus_tag="I9760_000817"
     CDS             complement(3894..5768)
                     /locus_tag="I9760_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAT5042603.1"
                     /translation="MLQKLNEHIQGVVAWLVIILIAITFTLFGVDYYFQSRQISDAKV
                     IVNDRPITMQAFETNYRRTRAQQDLPQMTAADEKNLQNQVLNQMITNEVSIQAARKYG
                     FEVSPEQANAAIVQIPQFQEDGHFSAQRYQQALSGALFTPETFQNEVRQGMLLNQQRF
                     AFMGTSFALSDEIKRFVRLYMQTRDYDYLTVPSDRFEQQAKISEDDIKSYYNQHHKKF
                     MTLEQVTLDYVLLSMHDIKSQIKISPDEIKSYYEENKSNYLTPAQWRVAHILFAVPEN
                     ATKEEQDSIKQKADEVYSDLKKHPEQFDKYVASKSDDKLSIANKGILPWITGGQNEYD
                     RVLSNLTRPGQISIPVQTKYGYEIFKLIAYKPVTTKSLQEVENIIMDQLTSYMAQAKY
                     AQVLEQLTDLSYQTPDSLDPVLDSLKLKIQHTEPFSRDGGKQPITKNKQVIHAAFSTD
                     VLELGNNSEPIQIDNDSVIVLRVKKHIPSREQTLDEVRDQIKKILAKQYGDAKAKEIG
                     SSLLHPVEDKQQQELIANNQFEWHSVENASRDSDKANSLINDLAFNLLRPESRDGVIL
                     DNGDYVVVKLKRINDGKLSSLDQEQRDSLIQQIEASYGMMDYDLYVNNLLHHAKIERK
                     "
     gene            complement(5860..5936)
                     /locus_tag="I9760_000818"
     tRNA            complement(5860..5936)
                     /locus_tag="I9760_000818"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5900..5902),aa:Asp,seq:gtc)
     gene            complement(5943..6018)
                     /locus_tag="I9760_000819"
     tRNA            complement(5943..6018)
                     /locus_tag="I9760_000819"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5983..5985),aa:Val,seq:tac)
     gene            complement(6026..6304)
                     /locus_tag="I9760_000820"
     CDS             complement(6026..6304)
                     /locus_tag="I9760_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAT5042604.1"
                     /translation="MNKSELVDAIASGSGLTKADASRVLETFMATVTDVLKKGDQVVI
                     PGFGSFSTGNRSARTGRNPQTGKTIQIKASRVAKFKAGKNLKEAVQQA"
     gene            complement(6433..8883)
                     /gene="lon"
                     /locus_tag="I9760_000821"
     CDS             complement(6433..8883)
                     /gene="lon"
                     /locus_tag="I9760_000821"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAT5042605.1"
                     /translation="MSNENEIISNETVKSSALPVLPLRDVVVYPHMVIPLFVGRGKSI
                     KALEAAMIDNKQIFLVAQRKSAHDDPGPEDIYQVGTVSSVLQLLKLPDGTVKVLVEGE
                     QRARVKEYTQDKGYLEATLEYIEEVGSTIQEQEIGILMRSLMSQFEQYIKLNKKIPPE
                     VLSPLAGIEEPGRLADTIAAHLTLKVDDKQELLETMDVGTRLEKLMAAIENEIDLLHV
                     EKRVRGRVKRQMEKSQREYYLNEQIKAIQKELGEMGEEGNEIEQLENSIKKAGMPKEA
                     KEKSLAELHKLKMMSPMSAEATVIRNYLDWMLMVPWKKRTKIQFDLLKAEKLLDKEHH
                     GLEQVKERIIEYLAVQQRVKRLKGPILCLVGPPGVGKTSLGQSIANATGRTFIRIALG
                     GVRDEAEIRGHRRTYIGSMPGKIIQKLCKAGVKNPLIMLDEVDKMAMDFRGDPASALL
                     EVLDPEQNHTFSDHYLEVDYDLSDVMFIATANSLEIPAPLLDRMEVIRLPGYTEDEKV
                     SIAEKYLVPKQMLLNGLTNEEIHISEGAIRDIIRYYTREAGVRNLERDIASICRKVVK
                     EILSSKKGKKGKTISITVSNITKYLGVKKYRYGLAEQYDQIGQVTGLAWTSVGGELLT
                     IEASIMPGKGKVTHTGQLGEVMQESIHAAMTVVRSRAKAFGLPDDFYEKHDFHVHVPE
                     GATPKDGPSAGIGMCTVLVSIVTQIPVKADVAMTGEITLRGQVLPIGGLKEKLLAAHR
                     GGIKHVIIPEENVKDLEEIPDNVLRKLTIHPVNTIEQVLELALQRNPWVEEPTNIQPG
                     TILNKRSKKIKNNDLHAH"
     gene            complement(9121..10395)
                     /gene="clpX"
                     /locus_tag="I9760_000822"
     CDS             complement(9121..10395)
                     /gene="clpX"
                     /locus_tag="I9760_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAT5042606.1"
                     /translation="MSKSGNGNGDKVLYCSFCGKSQHEVKKLIAGPSVFVCDECVELC
                     NDIIREETHETHEETEARLPTPKEISNFLDEYVIGQQHAKKVLSVAVYNHYKRLQHKS
                     EDGVELGKSNILLIGPTGSGKTLLAQTLARILNVPFAMADATTLTEAGYVGEDVENII
                     QKLLQKCDYDVDKAQQGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVA
                     SVPPQGGRKHPQQEFLQVDTSNILFICGGAFAGLDKVIRERSDKSSIGFSAQLKSKKS
                     SNDEVSKVLGQLESDDLIKYGLIPEFVGRLPVVTTLQELDEAALIDILTRPKNALTKQ
                     FQSLFKMEGSELEFRGEALIAIAKKALERKMGARGLRSILENILLDTMYDLPSLEGVN
                     KVVIDESVVNGLSKPILIYEQDEKKSASGSKD"
     gene            complement(10536..11180)
                     /gene="clpP"
                     /locus_tag="I9760_000823"
     CDS             complement(10536..11180)
                     /gene="clpP"
                     /locus_tag="I9760_000823"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAT5042607.1"
                     /translation="MPGYSDNIIRNASGLIPMVIEQTSRGERSYDIYSRLLKERIIFL
                     LGEVEDHMANLVVAQLLFLESENPEKDISLYINSPGGVVTAGLAIYDTMQFIKPDVST
                     LCIGQAASAAALLLCAGAEGKRFCLPNSRVMIHQPLGGYRGQATDIEIHARETLAVRE
                     RLNNIMAKHTKKTPDQIMRDTERDNFMSATQAMEYGLIDKVLYDRQVAGHSTDL"
     gene            complement(11183..12514)
                     /locus_tag="I9760_000824"
     CDS             complement(11183..12514)
                     /locus_tag="I9760_000824"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAT5042608.1"
                     /translation="MQVSVETLEGLERKVTVSVPTEKVEEEVSSRLRNLARKVKIDGF
                     RPGKVPFNVVKSRFSDSVREEVAREMVQSTLYEALQKNELVPAGYPHVEPLEIEPGKD
                     FKYTAVFEVMPVFEIVELNQAPVELIRSEVTDKDVDNMIEKLREQNKEWHEVTHAVKK
                     GDKVVIDFQGFLDDKPFQGGSAEGYELVIGSGSMIPGFEDGIVGGKIDKPFDIKVSFP
                     EDYGHKDLAGKEATFKITIKKIMEGKLPALDEAFAEKFNIKEGGIESLKKDIRENMAR
                     ELERRVNMMNREKLFDSLMSVNHVELPIALIDKEIEHLKHDMYHRLFGHEHKDDEKIP
                     DFPRELFEEQAKRRVHLGLLFAEYVKKHEIVADNDKVNAMIDKFASAYESPDELRAWY
                     QSSKEHMAEVEALVMEDMVADKIAEDAKLKYKNMDYDSVMNPKKGTEKKGE"
     gene            complement(12544..12628)
                     /locus_tag="I9760_000825"
     tRNA            complement(12544..12628)
                     /locus_tag="I9760_000825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12592..12594),aa:Leu,seq:tag)
     gene            complement(12719..12794)
                     /locus_tag="I9760_000826"
     tRNA            complement(12719..12794)
                     /locus_tag="I9760_000826"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12759..12761),aa:His,seq:gtg)
     gene            complement(12956..13032)
                     /locus_tag="I9760_000827"
     tRNA            complement(12956..13032)
                     /locus_tag="I9760_000827"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12996..12998),aa:Arg,seq:tct)
     gene            complement(13038..13114)
                     /locus_tag="I9760_000828"
     tRNA            complement(13038..13114)
                     /locus_tag="I9760_000828"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13078..13080),aa:Pro,seq:tgg)
     gene            13376..14602
                     /locus_tag="I9760_000829"
     CDS             13376..14602
                     /locus_tag="I9760_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT5042609.1"
                     /translation="MIMKTKLLPLFDNLNHIHKNHENVILPDARFHNDFTYCLSFLKS
                     YIGSEGTFNSYRRETERLLQWSWLVKNSTIDSLRRDDIEQYIHFCQNPPKTWISLKKV
                     PRFIEKNGNRIPNEEWRPFVVTLSKVSRKNGHLASIDQFKLSQGAVQEIFAILSTLFN
                     FLIAEEYLISNPVALIRQKSKFIRKRQHNAPVRRLSLVQWEAVLRAAESLAEDSPEKH
                     ERTRFILSLLFGMYLRISELAASERWVPSMNDFAKDSNGHWWFTTVGKGNKERQIAVS
                     DAMLESLTRWRLFLGLTPLPSPADNSPLLPKLRGHGPMSDTAPIRRIVQHCFDLAEEQ
                     LRLNGLSEEADNLSAATVHWLRHTGISEDVKIRPREHVRDDAGHSSSATTDRYIDIEK
                     QARYQSARKKIIQPSE"
     gene            14725..16575
                     /locus_tag="I9760_000830"
     CDS             14725..16575
                     /locus_tag="I9760_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT5042610.1"
                     /translation="MLTLRQITLSRGDKILLDKVNVSLYEKQKIGLIGHNGCGKSTLF
                     DFLLGQLSPDTGEYLINPQLSISHLSQHIPDSNEKALDFVLAGDQQYIKLLKRLSEAE
                     KNGDDAEVLQCHEELSHTGGYSKPAQAAVIMSGLGFSGNQQQEPVNSFSGGWRMRLNL
                     ARCLMKPADLLLLDEPTNHLDMEAIFWLERYLKQSPSTIILISHDREFLDAFVTHILH
                     IENRNLTLYSGNYSSFEKTHAQQLALQQAMHEKQQTKIKHMMTFVTRFKAKAAKAKQA
                     QSRMKAIEKMEIIAAAQADSPFSFDFFPCPRAGNPLIQCNQVDAGYINHPPILKKIKL
                     SLNPGDRIALLGPNGEGKSTLIKTLTADLPVLSGDIHRSPHLQIGYYAQHQLEQLNCK
                     LSPVETLQTLSPEVREQTIRDFLGGFNFRGDMAVQSIQHFSGGEKARLALARLVWLKP
                     NLLLLDEPTNHLDLGMRSAIELALQTYEGALILISHDRHLLKSSVDNFYLIYQGKVQP
                     FEGDLDDYYLWLQTRNSEKESKLATSPNEYKERKSYQNKLKKLEQLIEHYQNQLRLLD
                     KQLADVSLYEDTKQKSKLDNLLMERDIAHSSLMKTEEEWLEICNALENLE"
     gene            complement(16603..17277)
                     /gene="rnc"
                     /locus_tag="I9760_000831"
     CDS             complement(16603..17277)
                     /gene="rnc"
                     /locus_tag="I9760_000831"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAT5042611.1"
                     /translation="MKINLERLCRRLNYHFNNIAYLKQALTHCSAGSDNYERFEFLGD
                     SILSFVIANELFNRFPLHSEGQLSRLRSFLVKGEMLAEIAREIDLGDYLFLGQGELRS
                     GGFRRTSILADALEAILAAIYLDGGMIAAKQIILMLYSSRLDDPDLNHCLKDAKTQLQ
                     EFLQASKFALPEYVLTKIEGDEHAQIFHVTCTIEGVSQVAYGTGPNRRKAEQLAAKAM
                     LEQLQG"
     gene            complement(17267..17659)
                     /locus_tag="I9760_000832"
     CDS             complement(17267..17659)
                     /locus_tag="I9760_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HAT5042612.1"
                     /translation="MRKQQGMTLIGMLFTAVVVIMVGILVMRAVPVYIQHYSILHSIK
                     ALNSTPVSSLSGDPLTDVTTLRESVTKRLDINGVEDLKPGELVITPSGPNKFKIKLKY
                     QVIKPLIYNMSLLFNFEDNFEVITGSEN"
     gene            complement(17671..18426)
                     /gene="lepB"
                     /locus_tag="I9760_000833"
     CDS             complement(17671..18426)
                     /gene="lepB"
                     /locus_tag="I9760_000833"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAT5042613.1"
                     /translation="MNFALILVILSAVTGLIALLDWLIWSKKRLPEQKPGRMIEYSRS
                     FFPVFFIVLLLRSFLVEPFRIPSGSLEPTLLVGDFVAVNKFSYGLRLPVWEKKIVPIA
                     NPKTGEIAVFRWPPEPSYDYIKRVIGVPGDKISYHDKKLTINGKEVKQTFVEYTTDES
                     SGKAVTKYKEDLNGVVHDIFVRPDAPAVDFDIVVPEGNYFMMGDNRDDSADSRFWGFV
                     PDSYLRGKAFLVWMSWNGKTDNVRWSRIGRLIH"
     gene            complement(18534..20339)
                     /gene="lepA"
                     /locus_tag="I9760_000834"
     CDS             complement(18534..20339)
                     /gene="lepA"
                     /locus_tag="I9760_000834"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAT5042614.1"
                     /translation="MKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLTEREMSSQVL
                     DSMDIERERGITIKAQCVSLNYTAKDGKTYLLNFIDTPGHVDFSYEVSRSLAACEGAI
                     LVVDAAQGVEAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIEDIIGLDAQD
                     AIRVSAKSGLGVNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGVVSLVRIVNG
                     AIRKGDKMRVMSTGRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKEIQGAPVGDT
                     LTLDRNPADKVLPGFQRVKPQVYAGLFPVSSDDFEAFREALAKLSLNDASLFYEPESS
                     EALGFGFRCGFLGMLHMEIIQERLEREYNLDLISTAPTVVYQIVTQKGETLLIDNPSH
                     LPPTPQIKEMYEPIVRANILVPQDYLGPIITLCVERRGVQVSMTYSGRHVSVVYDIPM
                     SEVVSDFFDRLKSVSRGYASLDYNFQRFQIADLVKMDILINSERVDALAVIVHRDSAH
                     SRGKLIAEKMQQLIPRQMFDVAIQAAIGSHIIARQTVKALRKNVTAKCYGGDVTRKRK
                     LLEKQKAGKKRMKQVGHVEIPQEAFMAVFQTDKKK"
     gene            20561..21577
                     /gene="mltB"
                     /locus_tag="I9760_000835"
     CDS             20561..21577
                     /gene="mltB"
                     /locus_tag="I9760_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAT5042615.1"
                     /translation="MRQIIWLGFISLFLLSFTTYSDTTFTKRKDVQSFINMMVTNYHF
                     NRNQLTTVMNQVKLQPQVIESMEKPYEKKNWDVYRELFLTQKRLKSGLQFWAENQKTL
                     EKIQRRYGVSPEIIIAILGVETMYGERQGEYRVLDTLATLAFNYPKRSAFFTKELKEF
                     FLLCREQHVSPTQYKGSYAGAIGKPQFMPSSYRYYAVDFNNNGRRDLVNDNGDSIASI
                     ANYLHKHGWNLNQGIAQPAKISGWRYKQLRMNPKTANYRYSQLISYGVKPITASQNHP
                     PRAGVIELVTRKGKEYWIAYPNFFVITRYNSSPQYALAVYLLSQQLKQHWYTSATKKH
                     RAYV"
     gene            21652..22791
                     /gene="gspL"
                     /locus_tag="I9760_000836"
                     /gene_synonym="lspL"
     CDS             21652..22791
                     /gene="gspL"
                     /locus_tag="I9760_000836"
                     /gene_synonym="lspL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspL family type II secretion system protein
                     LspL"
                     /protein_id="HAT5042616.1"
                     /translation="MDKCFIFSKHLDDNGCVCLKISDLGELIAPPEFRNFSQIKDLQK
                     ESSTIIVETCTRAILLGLAIPWLPERKARIAIPYALEDKVTQPVEELHFAFDKFYYQN
                     NQYLVVVIDKQRMKKLIQVFDEHGIEFDAVTLDWFALASQELIISESELLINNEDFKG
                     VLSGDLAQTYLKNHPHTPAHLFQDSKIPVASPASQNEEHSYTWISKRLIKSKPLNLCQ
                     GEIQHGKTTDWVKKGYLLVGGLCGFWLISILFVNWLSLHWLNRQIDEIDRQTAVIYRE
                     FFPDAKQVISPKFRISQLLGGSTAENQTRFWFILNQLSKAIKDSGITVEQLRYQNKTI
                     SITLVSSDFESLQKIENKLKQSQLKVKQTQASTKDQQVVATLELT"
     gene            22788..23258
                     /locus_tag="I9760_000837"
     CDS             22788..23258
                     /locus_tag="I9760_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HAT5042617.1"
                     /translation="MKTYLNTLNDREKWMVIVAGVSLFIYGYYLLLYTPLSNQVNQKS
                     TQLIEKTETLEWMKQVRMQKRSAKRKESVDNSQLLTILASQLKNNKTLKFPYQLQQTG
                     SGDVQLTFDAVPFQNFIQWLAKINEVYSINIKQFDVEKTSTPGVTRLMIILSTS"
     gene            complement(23363..23896)
                     /locus_tag="I9760_000838"
     CDS             complement(23363..23896)
                     /locus_tag="I9760_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HAT5042618.1"
                     /translation="MMGISIRYIILGIGVMNISSCMVYEDTYTTRYQIYTYDYGQLYP
                     QTDYRARDYNYGNQSGQSVVVPDSYHVGEYHSPVSFKDRDRNWVNSQNPQGYTIQIAD
                     DEKAAYVAQKLYKAPKNDRMAQVKYYQNGKTHYKGLYGTYDSQEAAQKALESLPPEIK
                     QGAGIKDWGSVQGNLNE"
     gene            24170..25495
                     /locus_tag="I9760_000839"
     CDS             24170..25495
                     /locus_tag="I9760_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HAT5042619.1"
                     /translation="MWTHRRSGQTNQRGSQDHRDPYERDRTRVIHCPAFRRLQRKTQI
                     LGTDEGDFHRTRLTHSLEVDSIGRSIVRNLMTNQEHQLLLSSLLPNDDLISVICLLHD
                     IGHPPFGHGGEVALNYMMRHYGGFEGNGQTLRLLTKVENSYGGFGLDLTRRALLGILK
                     YPVKRANVVAPKQPPIHESIHKTIKINDWLPPKAYFDCEQPEVDWLLSPLSDNDRELF
                     QSLSVKPHGTQTGKAAYHNFDCSIMDTADDIAYGVHDLEDAIHLRLINRSHLDTPEFR
                     QLIGETTLSLHQERLLNSLFSPEICLRKQAIGEMVNYFITSTQIIVTNEEFENSLLKH
                     NIALIPEAHALLRYLMQCIYNNVIDSQEARTFEYGGQTVVLRLFDAISSNPASLLDNK
                     NRVLFSQAPDEVAAYRVVCDYLANMTDEYAYRMHERLFGFNTRTIFERL"
BASE COUNT         7943 a         5396 c         4406 g         7785 t
ORIGIN      
        1 atattttttg ctcaatcagt cagacacaat aaaaattaga agaaatttaa ataagctatt
       61 tattgttttt ataaaacatt tgatttaatt tacgcgtcaa tttgcaatat atgatggtca
      121 taaaaatgaa aaaaataagc tgcagtattc ttcttttgat caactcctta attagctacg
      181 ctgattcaga ccaacctaaa ttaattttac aaatagttgt tgaccaatta cgcggagatc
      241 tgatttaccg tcatcataaa cagtttggac cttctggttt caattatttg cttgctcatg
      301 gtttggatta tcataacgca catcatcctc atgccaatac aacgacctgc gcaggccata
      361 gcactattgc aacaggaagc tacccctctt tacacggcat agttgataat gattggtatg
      421 atagaaaaac aagaaaacta aagtactgta tggaggattt acaagccaaa attttaccca
      481 ctgtacacac ccatatagaa atttcaggac gctcaccgcg aaacctaaaa ccatccacat
      541 taagcgatga aatcatttta gcaaataaag ggaaagcctt tggtgtatca ttaaaagaca
      601 gggcagccat cactctagct ggtcacgcag gaaaagcgtt ttggtttgac aaaacgaatg
      661 gtggatttat tacaagcact tactactatt ccagttatcc actatgggta gaaaactgga
      721 ataaacacta tcaacccaaa gcctacgact ggaatctttc tcatcctaaa tcattctatc
      781 agaatgcaaa tactcttcca tttcgtcata actatggcag ctttggtcaa acatttcccc
      841 atcatgtaac caatcctcca tctgaagaat atttcaaatc gctttccaga acaccaaagg
      901 cagatgaatt aacagctgat tttgccaaac aactactggt taatgaaaaa ttgggaaaaa
      961 caacaggcca aacagattat cttgcaatca gtttttctgc tgtagatgct attggtcacc
     1021 aatttgggcc taatagtctg gaagcagaag acaatttgat cgcccttgat gacaccctaa
     1081 gccaactttt taaaaccatt gataaagagg ttggtcttaa taatacctta ataatactca
     1141 ccgcagatca tggtgtcagc gatggtccat cttaccttaa agcacataaa atacctgaaa
     1201 ttaaacctgt taatatagaa gcaacaggaa aacacatcga gaatttatta ttgaaacgtt
     1261 tcaacctgcc tgcgcaagct ttaatgtcaa ttaatcctcc ttttatatac cttgatcacc
     1321 aaattataaa agagaaaaat ttggatattg cagaggtcag tttctacctg gcagaagaaa
     1381 tcagtcatct tccagggata ttcaaagcct accccttacc tattcgtgat attgaaaagg
     1441 attggttaag cgcaaaagtt gaccggatgt cttacgctta ccgcgcaggc gatgtttaca
     1501 ttgttcaacc gccatatcaa agccacggtg caaaaagtga agatcgagtt gctcatggca
     1561 gtccctggca atatgatact tatgtcccgc tactgtttgt acaccctgac ttcaaaccaa
     1621 aacgtatatt caggcaagta cacacaacgg atattgcacc aacattatct tccttattat
     1681 tgattaaacc ccctgccgca gcagtaggac agcctttagt tgaagtagtc aatgcttttc
     1741 ataagactgg agagaatgat tgaattccaa ctgaaaaaac tttactattc gtacaaaatt
     1801 tttaatgaaa acattatgac aaagcaagca attattattg ggatttccgg cccatcagca
     1861 tctggaaaaa gtttattagc aaatactata gtcaatgaat taggatccga gcaggttgtc
     1921 gttatctctg aagatgccta ctataaagat aatgggcatc taccctttac agagagagaa
     1981 aaaataaatt acgatcatcc tgactccttt gatcatgctc tactttatga gcatcttcgt
     2041 cagctacgag ttggaaatac agtacaaatt cctatttatt ctcattccaa acatctgcgt
     2101 ttacctgaaa ccagagcagt gggtcaacat gccattattg tactcgaagg aatactgctt
     2161 ttcagtgata aagctctacg agaaattatg gatatacgta tattcatgtc gactcctctt
     2221 gacgtctgtt taacgcggcg cttaaaaaga gatgtagtag aaaggcatcg atcatttgaa
     2281 tcggttattc atcaatatga gactacagtt cgccctatgt acatgcaatt tattgagcct
     2341 tcaagccgct acgctgatat aatagtacct cgcggcggtg aaaacagaat cgctatagag
     2401 atgattcaag ctaaaatgcg ggaattatta gcttcacata ctcgaggcag ttgatgtttt
     2461 attaggctgt ttaagagcaa cctaattgat attgtggttt tagtccagga tcgaagtaac
     2521 actcacttcg ccctgctaaa ttaagaataa tgggaggaga tactattgtg ggatttttaa
     2581 ctggaaaaaa agcacttatt gttggattgg caagcaacag atcgattgca tatggcattg
     2641 ctaaagcatt ccataatcaa ggcgctgagt tggcttttac ttaccaaaac gaaaaattac
     2701 agtcacgtgt tgaaacaatg gcatcggaat ttaattcaac tctagttttt ccttgtgatg
     2761 ttgcaagtga tgaagaaata aaagccgtgt ttgataattt gcgtaatcac tgggataaat
     2821 tagatatcct ggtccactct gtagcttatg ctcctgctga tcagatcagt ggtgattttg
     2881 tagaatgtgc aaatagagaa ggatttagaa tagctcatga tattagtgcc tatagcctca
     2941 taggtttgtc tcaagccgca ttgcctatga tgctagatac tcagggttca attttaacct
     3001 taagctatta tggggcagaa aaagccgtac caaactataa cgttatgggt gttgcgaagg
     3061 caagcttgga agcctctgtg cgctatctgg cagccagctt aggatccaga ggattaagaa
     3121 ttaatgccat atcagctgga ccaattaaaa ctcttgccgc tgctggaatt aaagattttc
     3181 gtaaaattca tgctgcctat gcaaacatta ctccattaca aagaaatgtt actgctgatg
     3241 aagtcggaaa tactgctgcc ttcttatgct ctgatctggc atccgggatt actggtgaag
     3301 tgctacatgt tgatgcagga tatcacgcag tatcagcgat gagcgaacta ggttagatat
     3361 atattagtcc gctttaaatt gctgacttaa ttcttagttg tacttaaaaa aattcaataa
     3421 agctaaatta tattggggct gagcaaatag cgaggcccag actactaaac gttttttata
     3481 atataacaga caaatttaga tttagcctat ctaatctatt gaatcgacat gaaatagagc
     3541 acctcaatca aatgactcct gcgactcctt cattaacgga ttattattaa gagaataaac
     3601 ctcaacttaa aaagaccata tttagctgat tttgaatgaa aataatcctt caaaagctca
     3661 tataaccacc tgcgctctat tcaggatcat tttcgtccag cctgaacccc aaaccaaccg
     3721 ctataaaata tttcaacgcg taagtctatg aacagcttca ggcttattaa tgtgatattt
     3781 atatcaggag caactttgaa aaagcctcac agagaaaaac tataggcaaa aactgaagaa
     3841 ttctcgatat ctggccatcc cttaatttcg taaggaacag ccattttctt atcctatttc
     3901 ctttctattt tcgcatgatg aagcaagtta ttaacatata aatcataatc catcattcca
     3961 tagctagctt ctatttgctg tatcaagcta tctctttgct cttgatccag agagcttaat
     4021 tttccatcat taattcgttt taatttaaca acaacataat cgccgttgtc taaaattact
     4081 ccgtctcggc tttctggacg taataaatta aatgctaaat cattaattaa agaatttgct
     4141 ttatcactat ccctggaagc attctctaca gaatgccatt cgaattgatt gttagcgatt
     4201 aattcctgct gttgcttatc ttcaacgggg tgaagcaaac tactaccaat ttcttttgct
     4261 ttagcatccc catactgttt tgcaagtatt tttttaatct gatctcgcac ttcatccaag
     4321 gtttgttctc ttgaaggtat atgtttcttc actcgtaaaa ctattacaga gtcattatca
     4381 atctgtatag gctcgctatt attccctagt tctaaaacat cagtactgaa tgctgcatgg
     4441 ataacctgct tatttttggt aataggttgc ttgccaccat cacgagaaaa aggctctgta
     4501 tgttgaattt tcagctttaa tgaatccaaa accggatcaa gtgaatcagg agtttgataa
     4561 cttaaatctg ttaattgctc taaaacttga gcgtattttg cctgagccat ataactggtt
     4621 agttgatcca ttataatgtt ttcaacttct tgcaaagatt tagttgtaac tggcttgtaa
     4681 gcaatcagct taaaaatttc ataaccatat tttgtttgta ctggtataga aatttgccct
     4741 ggtctagtta aattggaaag tactctgtca tattcatttt gcccgccagt aatccaggga
     4801 agaataccct tattggctat agataacttg tcatcagatt tggaagcaac atatttatca
     4861 aattgttctg gatgcttttt taaatcgcta taaacctcat cggctttttg cttaattgaa
     4921 tcttgttctt ccttggtggc attttctgga actgcaaata aaatatgggc aacccgccat
     4981 tgagcaggag ttaggtagtt acttttgttt tcttcataat agcttttaat ttcgtcgggt
     5041 gatatcttta tttgtgactt gatatcatgc atagacaaaa gcacataatc caatgtaact
     5101 tgctctaaag tcataaattt tttatggtgt tggttataat aactcttgat atcatcttct
     5161 gaaatcttcg cctgttgttc aaagcgatca gaaggtaccg tcaaataatc ataatccctg
     5221 gtctgcatgt acaatctgac aaaacgcttt atttcatctg ataaagcaaa tgatgttccc
     5281 ataaacgcaa aacgttgttg attaagcaac atgccttgcc ttacttcatt ctggaaagtc
     5341 tctggagtaa acaacgctcc actcaaagct tgttgatatc gttgtgccga aaaatgtcca
     5401 tcctcctgga attgaggaat ttgcactatg gctgcattgg cttgttctgg gctaacctca
     5461 aatccatatt tcctggcagc ctgaatactc acctcattcg ttatcatttg atttaatact
     5521 tgattttgca gatttttttc atcagcggca gtcatctgtg gtaaatcttg ctgggcacga
     5581 gtacgtctgt agttcgtttc aaacgcttgc atggtaattg gtctatcatt aactattacc
     5641 tttgcatcgg atatttgtcg agattgaaaa taataatcaa caccaaaaag tgtaaaggta
     5701 atagcaatta atataataac taaccacgct actacaccct gaatgtgttc atttaacttc
     5761 tgcaacatgt cccgagatcc attttgatat taacaatgct tgatgaaacc gattctacca
     5821 taagctgata aaaaaaacgc gcctttcagc gcgtttatct ggcggagtgg acggggctcg
     5881 aacccgcgac ccccggcgtg acaggccggt attctgacca actgaactac cactccattt
     5941 cttggtgggt gctgtaggga tcgaacctac gaccctcgcc ttgtaagggc gatgctctcc
     6001 cagctgagct aagcaccctg aaaacttaag cttgttgtac cgcttctttc aggtttttac
     6061 ctgctttaaa tttggcaacc cgtgacgctt taatttggat tgtttttcca gtctgtgggt
     6121 ttctgccagt acgtgctgaa cgattacctg ttgaaaaaga accaaaacca ggtattacaa
     6181 cttgatcgcc tttctttaag acatcagtta cagtagccat aaaagtttca agaactctgc
     6241 ttgcgtcagc tttggtcaaa cctgaaccgc ttgctatagc atcaactaat tcactcttat
     6301 tcatcttttc ccctttacag ttaatcatcc atattccgga gctgatttaa acagattcaa
     6361 ctgctccagt caagttcgta catccttgca gagcccgcca gtagcgggcc acgaagagaa
     6421 atatactctg tattaatgtg cgtgtaaatc attattttta attttttttg atcttttatt
     6481 caatatagtg ccaggttgta tatttgttgg ctcctccacc catggattgc gttgcaaagc
     6541 cagttccaaa acctgttcaa tggtgttaac aggatgaatt gtcagcttgc ggagcacatt
     6601 atcagggatt tcctcaaggt cttttacatt ctcctcgggg ataatgacat gcttgattcc
     6661 accacgatgt gcagccagca atttttcttt taatcctcca attggtaaaa cttgcccacg
     6721 cagagtaatt tcacctgtca tggcgacatc tgccttaact gggatttgtg ttactatgga
     6781 gactagtacc gtgcacattc ctataccagc gcttgggcca tctttgggtg ttgcaccttc
     6841 tggtacatga acatgaaaat catgtttctc ataaaaatca tctggcaaac caaaagcttt
     6901 agcgcggctt cttactaccg tcatagctgc atggatagat tcttgcatta cctcaccgag
     6961 ctgaccagta tgagtaactt tacccttacc tggcataatg gatgcttcta tagtcagaag
     7021 ttcacctcca acacttgtcc aggctaagcc agtgacttga cctatctgat cgtattgttc
     7081 agctaatcca taacgatatt ttttaacgcc caaatactta gtaatgttgc taacagttat
     7141 actgattgtt tttccttttt ttcccttttt gcttgagagt atttctttaa caacttttct
     7201 gcatatactc gctatatccc tttcaagatt acgtactcca gcctctcgag tataataacg
     7261 gatgatatct ctaatagccc cttcgctaat atggatttct tcattggtta aaccatttaa
     7321 cagcatttgt ttgggaacca aatatttttc agcaatactg actttttcgt cctcagtata
     7381 tccaggcaag cgaataactt ccattctatc caataacggg gccggaattt ctagcgaatt
     7441 ggcagttgca ataaacataa catcactcaa gtcataatca acttccaagt aatgatcact
     7501 aaatgtatgg ttctgttcag gatccaatac ttccagtaat gccgatgccg gatcaccgcg
     7561 aaaatccatt gccatcttat ctacttcatc cagcatgata agaggatttt taactcctgc
     7621 cttgcataat ttttggataa ttttacctgg catggaacct atatatgttc ttcgatgacc
     7681 acgaatttct gcttcatctc ttaccccgcc cagagcgatg cgtatgaaag ttcttccagt
     7741 agcattggcg atagattgtc caagagaagt tttacctact cccggaggac ctactagaca
     7801 caatatcggt cctttcaatc tttttactcg ttgttgtaca gcaagatatt cgatgatacg
     7861 ttctttaacc tgctccagac catgatgctc cttatcgagc agcttttctg cttttagtaa
     7921 atcaaactga attttggtcc gcttcttcca tgggaccatc agcatccaat caagataatt
     7981 acgaataaca gttgcttcag cagacatcgg agacatcatt tttaatttat gtaactctgc
     8041 taatgatttc tctttagctt ctttcggcat tccagccttt ttgattgaat tttccaattg
     8101 ttcaatctca ttgccttcct cacccatttc ccctaattct ttctgaatag ctttgatttg
     8161 ttcgtttaga tagtattctc tctggctttt ttccatttga cgtttgactc gacctcttac
     8221 acgtttttcc acatgtaaca gatcaatttc attttctatc gccgccatta atttctcaag
     8281 tcgagttcct acatccatag tttcaagcaa ctcttgctta tcatctactt taagcgttaa
     8341 atgtgcggca atggtatctg ccaggcgacc tggctcttct attcctgcca atggtgacaa
     8401 gacttctggt ggaattttct tattaagctt tatatattgt tcaaattgcg acatcaagga
     8461 ccgcattaga attccaattt cttgctcttg aattgttgag ccaacttcct caatgtattc
     8521 aagtgtcgct tcgaggtaac ctttatcctg agtatactcc ttgacacgtg cacgctgctc
     8581 accttcaaca agaactttta cagttccgtc aggtaacttt aacaattgca aaacactgga
     8641 tacagttccc acttgataga tatcttctgg accaggatca tcatgcgccg atttacgctg
     8701 agcaactaaa aaaatttgtt tattatcaat catcgctgct tcaagagctt taattgactt
     8761 gccacgccca acaaagagag gtatcaccat atgcggatag acgactacgt ccctcaatgg
     8821 taatacaggc aaagcagaag atttcacagt ctcatttgat attatttcat tttcattaga
     8881 cataatgacc cttattcttc aaaggaaaat ttcttggtta tagtttctta atactattac
     8941 ttacgagtat acagatattg gtgctggtga acaattcgta atgtgagagt tagtctattg
     9001 ctattaagat ggctaaattt gttattttaa gctacaaaat cagctttaaa tatattttgt
     9061 gaatgaaaga taaaataact tctgcaacaa tttaaattat tgcagaagtc aacatcttac
     9121 ttaatcttta ctaccagacg cacttttttt ctcatcctgt tcataaatga gaatcggttt
     9181 agatagtcca ttcactacac tctcatcaat aacaacctta ttcacgcctt ccaaggaagg
     9241 taagtcatac atagtgtcta aaagaatatt ttcaagtatt gatcgcaaac ctcttgcgcc
     9301 catttttctt tcgagggcct ttttagcaat agcaatcaaa gcttcgcctc taaactcaag
     9361 ttcagaacct tccattttga aaagtgattg aaattgcttt gttaaagcat tcttaggtct
     9421 cgttaatata tcaatcaatg ccgcttcatc aagttcctgt aaagtggtta caacaggcaa
     9481 acgtccaaca aactcgggaa tcaacccata tttaattaaa tcatctgatt ccaattgacc
     9541 taaaactttt gaaacttcat cattactgct ttttttgctt tttaattgag cagaaaaacc
     9601 tatgcttgat ttatcgctgc gttcacgtat tactttgtcc aaacctgcaa aagcgccacc
     9661 acagataaat aaaatatttg atgtatctac ctgcaaaaat tcctgctgtg gatgctttcg
     9721 cccaccttgc ggtggaacag atgcaacagt accttcaatt aatttcagta aagcttgttg
     9781 aacaccttct cctgaaacat ctctggtgat agatggatta tctgatttgc gtgaaatctt
     9841 atcgatttcg tcgatataga caataccttg ctgtgcctta tctacatcat agtcgcactt
     9901 ctgtaataac ttctggataa tattttcaac gtcttcgcca acatatccag cttcagtaag
     9961 ggtcgtggca tcagccatgg caaaaggaac attaagaatc cgcgccaggg tttgagctaa
    10021 aagagtctta ccactgccag taggtcctat aagtaaaata ttacttttgc cgagctcaac
    10081 accatcctcg cttttatgct gtaatctctt atagtgatta tatactgcta ctgataaaac
    10141 ctttttggca tgttgttgcc ctattacgta ttcatcgaga aagttagata tttcttttgg
    10201 tgtcggtaga cgtgcctcag tttcttcatg agtttcatgc gtctcttctc gaataatatc
    10261 attacaaagc tctacacatt catcacaaac aaacacagaa ggtccagcaa ttaatttttt
    10321 tacctcatgt tgactcttgc cacaaaaaga gcaatataag accttatctc catttccgtt
    10381 accggattta ctcataacca aaccccttct tacagctatt actaattaat agtaattaca
    10441 tgtaaaattt tagacaatca gataatattg cgccaccaca ttactatcaa accaacttat
    10501 attatactca gataaactag gagtatctag caaagttata agtctgtaga atgtccagca
    10561 acttgtctgt catagagaac tttatcgatt agaccatact ccatagcctg agtagcactc
    10621 ataaagttat ctctttctgt atcacgcatg atttgatcag gtgttttttt agtgtgttta
    10681 gccataatat tattcaaacg ttccctaact gccaaggtct ctcgagcatg aatttctatg
    10741 tctgttgctt gacctctata gccacccaag ggctgatgta tcataactct tgagttaggt
    10801 aagcagaatc gtttgccttc agcaccggca caaagtagca aggcagctgc acttgctgcc
    10861 tggcctatgc ataatgtact tacatctggc ttaataaatt gcatggtgtc gtagatagca
    10921 agacctgcag taacgactcc gccgggagaa ttaatatata gagatatatc tttctctgga
    10981 ttttcagact ccagaaacaa caactgagcc acaaccaggt tagccatatg atcttctact
    11041 tcacccaaga gaaaaataat tctttctttt aataatcttg agtaaatgtc ataggaacgc
    11101 tcacctcgcg aggtttgctc aatgaccatt ggtattaatc cgcttgcatt acggataata
    11161 ttatctgaat agcctggcat gcttactctc cttttttctc agtgcccttt ttgggattca
    11221 tcactgaatc ataatccata tttttgtatt taagtttcgc atcttctgct attttatcag
    11281 caaccatatc ttccataact aatgcttcaa cttcagccat atgctctttg ctgctttgat
    11341 accacgcgcg aagctcatca ggactttcgt aagcactggc aaatttatca atcatagcat
    11401 taactttgtc gttgtctgca acaatttcgt gtttttttac atactcggca aaaagtaagc
    11461 ccaagtgtac gcgtcgcttg gcttgctctt caaataactc ccttgggaaa tcaggaattt
    11521 tttcatcgtc tttatgttcg tgaccaaaca atcggtgata catatcatgc tttaaatgct
    11581 caatttcttt atctatcaaa gctattggca actcaacatg attcacagac attaatgaat
    11641 caaacagttt ctctctgttc atcatgttga cccgacgttc taattcacga gccatatttt
    11701 ctctaatatc ctttttaaga gactctatac caccttcttt tatattaaat ttctctgcaa
    11761 atgcctcgtc caaagcaggc aacttgcctt ccataatctt tttgatagtg atcttgaacg
    11821 ttgcttcctt cccagccaaa tctttatggc cataatcttc cgggaaactc actttgatat
    11881 cgaacggttt gtcaattttc ccaccaacaa tcccatcttc aaatccggga atcatcgaac
    11941 cagaaccaat tacaagctca tatccttcag cactgccgcc ctggaatggc ttatcatcca
    12001 aaaatccctg aaaatcaata acaactttat cacctttttt gaccgcatga gttacttcat
    12061 gccactcttt gttttgttct cgtaattttt caatcatgtt gtcaacatct ttatcagtga
    12121 cttcagatcg aattaattcc actggagctt gattgagctc gacaatttca aaaacgggca
    12181 taacttcaaa tacagcagtg tatttaaaat ccttacctgg ttcgatttct aaaggctcaa
    12241 catgaggata acctgctgga accaattcat ttttttgtaa ggcttcatat aaggtagatt
    12301 ggaccatttc ccgggcaact tcttcacgca cactatcaga gaaacggctt tttacaacat
    12361 taaaagggac tttgcctgga cgaaaaccat ctattttcac tttgcgagca agatttctga
    12421 gacgcgagct cacctcttcc tcaactttct ctgtaggtac agaaactgta accttacgct
    12481 ccaaaccttc tagggtctca acagaaactt gcattaattc acctctcgga agttatcatg
    12541 aaatggtgcg aaaggagaga ctcgaactct cacgggtcgc cccgctggaa cctaaatcca
    12601 gtgcgtctac caattccgcc actttcgcaa tcaggttgga ttatcaatca gtaattcagt
    12661 cttgtaaaga ctcagatgga atctttagcg gtagactcaa aaaaatattt gttacaaatg
    12721 gggtgaacga tgggactcga acccacgaca accgggatca caacccgggg ctctaccaac
    12781 tgagctacgc tcaccaaaaa cactcttaaa cagggcttac gaaacttccc aaattacaaa
    12841 ttcataattt tctatatagt tatttacaca aaatagattt tcattaaaga attacgtaac
    12901 tatagatggt ttcgtaagcc ctgttccagc tataatactg caaaagcaag gcggctggcg
    12961 cgcccggcag gattcgaacc tgctaccctc ggcttagaag gccgatgctc tatccagatg
    13021 agctacgggc gcttaaatgg tcggggagga gggatttgaa cccccgacat cctgctccca
    13081 aagcaggcgc gctaccagac tgcgctacac cccgataccc gtcccaagga cagagcgcag
    13141 ataatactag gtgctatcac gaaggtcaat atcctttagc atataatttt tatttattga
    13201 tatagtctga atttcttcag tacttttcgt caatttattg ccctttacaa gcgcttacgt
    13261 agttgcctca gcactattat caacaataac tcgtgtactc tataactatg cactttcacc
    13321 ctcatgactt aaatcagcaa ttaggttagg ataatgaatt atcagcagat aagttatgat
    13381 catgaaaaca aaactattac ccttgtttga taatctcaat cacatccata aaaaccatga
    13441 aaatgtaata ttgcctgatg cccgatttca caatgatttc acttattgct taagcttttt
    13501 aaaaagctac atagggagcg aaggtacttt caacagttac agaagagaaa cagaacgttt
    13561 gttacagtgg tcatggcttg tcaaaaactc tactattgat tctttaaggc gcgacgatat
    13621 tgaacaatac atccatttct gtcaaaaccc gcctaaaaca tggattagtc ttaaaaaagt
    13681 tccacgattt atagaaaaaa atggtaaccg gatacctaat gaagagtggc gaccttttgt
    13741 agtgacacta agtaaggttt ccaggaaaaa tgggcatctt gcatcaatag atcaattcaa
    13801 gttatctcag ggagccgtgc aagaaatatt cgccatctta agtactctat tcaactttct
    13861 aattgctgaa gaatatctga tatccaatcc agttgccctt attaggcaaa aaagcaaatt
    13921 tattcgcaaa agacagcata acgcgcctgt tagacgatta agtctggttc aatgggaggc
    13981 tgtcctgaga gcagctgaaa gtttggctga agattcccct gaaaaacatg aacgcactcg
    14041 ttttatttta agtctgttat ttggcatgta tcttcgtata tcagaacttg ctgccagtga
    14101 gagatgggta cccagtatga atgattttgc caaggacagt aatggccact ggtggtttac
    14161 taccgtaggg aaaggaaata aggaacgaca aattgcggta agcgacgcta tgctggaaag
    14221 tcttacccgt tggcgattat ttcttggact tacccctctt ccatcaccag ctgataattc
    14281 gcctttacta cctaagctgc gtggtcatgg tccaatgagt gatacagctc ctataaggcg
    14341 aattgttcag cattgttttg acttggcaga agaacaactg cgacttaatg gtttatcaga
    14401 ggaagcggat aatctatccg ctgccacggt gcattggctg cggcatactg gtatatcaga
    14461 agatgtaaaa atcaggccca gagagcatgt gcgagatgat gccgggcaca gttcaagtgc
    14521 tacaacggac aggtatattg atatagaaaa gcaagcacgt tatcaatcgg cacgaaagaa
    14581 aataattcag cccagcgaat aatttatttt taaagaaaat ctggttttaa acttatgcaa
    14641 atatccagct cgcttacatg atcaatagga agcttaagct aatttatgat aaaatgactg
    14701 attagttcaa gcttacggta taacatgctt acattgcgtc aaattacttt atccagagga
    14761 gataaaattt tactcgataa ggtcaatgtc agtctttatg aaaaacaaaa aattggcctt
    14821 atcggtcata atggatgtgg aaaatcaaca ttatttgatt ttcttttagg tcaattatct
    14881 cctgatactg gagaatatct aattaatcca caactcagca tcagtcattt gtctcaacac
    14941 atacctgata gcaatgaaaa agctttggat tttgttcttg ccggagatca acaatacatt
    15001 aaattgctaa agcgcctttc agaagctgaa aaaaacggcg atgatgcaga ggttttgcaa
    15061 tgtcacgagg aactctctca tactggtggt tacagtaagc ccgcgcaagc agcagttatt
    15121 atgtctggct taggatttag tggaaatcaa cagcaagagc ccgttaacag cttttctggt
    15181 ggctggagaa tgcgcttaaa tcttgctcgg tgcttaatga aacccgccga tttactcctg
    15241 cttgatgaac ccacaaacca tttagacatg gaagccatct tctggctgga acgctattta
    15301 aaacaaagtc caagtactat tattcttatt tcacatgata gagaatttct tgatgccttt
    15361 gttactcata tattacatat agaaaatcgt aatctgacac tttattcagg aaactatagc
    15421 agctttgaaa aaactcatgc gcaacaactg gctctgcaac aagcaatgca tgaaaagcaa
    15481 caaaccaaaa tcaaacatat gatgactttt gtaactcgtt ttaaagcgaa agcagccaag
    15541 gccaaacaag cgcaaagtcg aatgaaagct atagaaaaga tggaaataat tgcagcagcc
    15601 caagccgact cccctttttc ttttgatttt ttcccttgtc ctcgggcagg aaatccttta
    15661 attcaatgca atcaagttga tgcaggatat atcaatcatc ctccgatttt gaaaaaaatc
    15721 aaactctctc ttaatcccgg cgatagaatt gcgctactgg gacctaacgg tgaaggaaaa
    15781 tcaaccttaa ttaaaacgct tacagcagat ttgcccgtat taagtggaga tattcatcgt
    15841 tctccacact tacaaatagg ctactacgct cagcatcaac ttgagcaatt aaactgtaaa
    15901 ttaagtccgg tagaaacact gcaaactctc tcgccagagg taagagaaca aaccatacgg
    15961 gattttttag gtgggtttaa tttcagaggg gacatggcgg tacaatccat tcagcacttt
    16021 tcaggtggtg aaaaagcccg cctggcatta gcaagactgg tttggctaaa acccaatctt
    16081 cttttgctgg atgagccaac aaaccatttg gatcttggaa tgcgctcagc aattgaatta
    16141 gcgctgcaaa cttatgaggg tgccttaatt ttgatttcgc atgaccgcca tttgttaaaa
    16201 tcaagtgtgg acaactttta tcttatttat caaggaaaag tacaaccatt tgaaggggat
    16261 cttgatgatt actatctctg gctgcaaacc agaaattcag aaaaggaatc caaattagct
    16321 acttccccaa atgaatataa agaaagaaaa agctatcaaa acaaattaaa aaagctggag
    16381 caactcattg agcactatca gaatcaattg cgccttcttg ataagcagtt agctgacgtt
    16441 tctctgtatg aagacacaaa gcagaaaagc aaattggata atcttttgat ggaacgagat
    16501 atcgctcatt cttctttaat gaagacagaa gaagagtggt tagaaatatg taatgctctg
    16561 gaaaacctgg agtaagtaat aggttctgct aatcaatacc atttaacctt gtaactgttc
    16621 caacatggct tttgctgcca actgctcagc ttttcttcta ttaggccctg tgccataagc
    16681 tacctgactt actccctcaa ttgtgcatgt tacatggaag atttgagcgt gctcatcccc
    16741 ctcaatcttg gttaggacgt attcaggtaa agcgaatttt gatgcttgca aaaactcctg
    16801 taattgtgtc tttgcatcct ttaaacaatg atttaaatcc ggatcatcca atcgtgaact
    16861 ataaagcatt aaaataatct gttttgcagc aatcattcct ccatccaaat aaatggctgc
    16921 caatatagcc tcaagggcat ctgctaatat agatgtgcgg cgaaatccac cgctccttaa
    16981 ctcaccttgc ccaagaaata gataatcacc caaatctatt tctctggcta tttctgctaa
    17041 catttcacct ttaacaagaa aagaacgcaa tcgacttaat tgcccttcac tgtgaagtgg
    17101 aaaacgattg aatagctcat tggcaataac aaaacttagt atcgaatctc caagaaactc
    17161 aaagcgttca taattatcgc taccagcact gcaatgcgtc aaagcctgct tcaaataggc
    17221 tatgttatta aaatgatagt ttaaacgtct gcacaatctt tctaaattaa ttttcactac
    17281 ctgttattac ctcaaaatta tcttcaaaat taaataataa gctcatatta taaattagag
    17341 gtttgatgac ttgatatttt aacttaattt taaatttatt ggggcctgaa ggagtaataa
    17401 ccagctctcc tggttttaag tcctctaccc cattgatatc taatcgttta gtcacacttt
    17461 cccttaaagt tgtaacatcg gtcaagggat cccccgacaa agaagaaaca ggtgttgaat
    17521 ttaatgcttt gattgaatgc aaaatcgaat aatgttgaat ataaactggc actgcccgca
    17581 taacaagtat accaaccatt atcaccacaa cagcagtaaa cagcatacca atcaatgtca
    17641 ttccctgttg ttttctcatg ccctattccc tcaatgaatt aatctgccta tccttgacca
    17701 gcgtacatta tctgttttgc cattccaact catccagacc agaaatgctt ttcctcttaa
    17761 atagctatca ggtacaaacc cccagaaccg actatcggcg ctatcatccc gattatctcc
    17821 catcataaag taattcccct caggaacaac tatatcaaaa tctacggctg gcgcatcagg
    17881 tctgacaaat atgtcgtgca ccacaccgtt taaatcttct ttatactttg tcacagcctt
    17941 gccagagctt tcatcagtag tatactcaac aaaggtctgt ttcacctctt tgccattaat
    18001 agttagtttt ttatcatgat atgaaatttt atccccaggc actccaataa ctcttttaat
    18061 atagtcataa gatggttcag gtggccagcg aaatactgca atttctcccg ttttgggatt
    18121 agcaattggc actatttttt tctcccatac aggaagtctc agtccataag aaaatttatt
    18181 aaccgctaca aaatcaccaa ccaacaaagt aggctcgagt gagcccgatg gaatacgaaa
    18241 aggttcaact aaaaaagagc gcaatagcag aacaataaaa aatacaggaa aaaaagaacg
    18301 tgaatactca atcattcgac ctggcttttg ttcaggcaaa cgctttttag accaaatcaa
    18361 ccaatccaat aaagcaatca atcctgttac agcggataat atgaccaata ttaaagcaaa
    18421 attcataaat tcaatatcct caattttaag caaaagacaa tttaatcatg atttgactta
    18481 tgtccagatg ctgacaaaaa ggtcaacaca tccttattag taaatgagcg gctctacttt
    18541 tttttatctg tttgaaacac ggccataaat gcttcttgag gtatttctac atgccccacc
    18601 tgtttcattc ttttcttgcc agctttttgt ttttccagta atttccgttt acgcgtcaca
    18661 tcaccaccat aacactttgc tgtaacgttt ttgcgcaatg ctttcactgt ttgcctagca
    18721 ataatatggc ttcctatagc agcctgaata gccacatcaa acatctgtct tggaataagc
    18781 tgttgcatct tctccgcaat caattttccg cggctatgag cggagtctct atgaacaatc
    18841 acagcaagag catcaacacg ttcgctattt attaatatat ccatcttgac taaatcagca
    18901 atctggaatc tttggaagtt ataatccaaa gaggcataac ctcgactaac tgattttaat
    18961 ctatcaaaga aatcagagac tacttcgctc attgggatat cataaacaac tgaaacatga
    19021 cgaccactat aggtcatact gacttgaaca ccacgtcttt caacacataa ggtaatgata
    19081 ggccccaaat aatcttgtgg cactagaata tttgctctga caattggctc atacatctct
    19141 tttatttgcg gggttggggg caaatgagaa ggattatcaa ttaataaagt ttcccccttc
    19201 tgagttacaa tctgataaac aacagtaggc gccgtagaaa tcaaatcaag gttatattct
    19261 ctttccaatc tctcctgaat gatttccata tgcagcatgc ctaaaaatcc gcaacgaaaa
    19321 ccaaatccca aagcctctga agattccggc tcataaaata aagaagcatc atttaaactc
    19381 aatttagcca aagcctctct aaatgcctca aaatcatccg agctgaccgg aaacaaacca
    19441 gcataaactt ggggcttaac gcgttgaaac cctggaagaa ctttatcagc tggatttcta
    19501 tccagagtta aagtatcacc aactggagcg ccctgaattt cctttatacc agcaacaaca
    19561 taacccactt caccagcata taaagcatcc agtttagtcc gtttgggggt aaaaatacca
    19621 acttgatcca cttcataagc tctgcctgtg gacatcactc gcattttatc accttttcgt
    19681 atggcaccat taacaatacg aaccagtgag acaactccta aatagctatc aaaccaggag
    19741 tcaataataa gtgcttgtaa tggggcatga acatcgcctt tcggaggggg aatattcgca
    19801 accaatgcct ctaaaacatc attaacaccc aagccacttt tagcacttac tctaatcgca
    19861 tcttgagcat ctagaccaat gatatcttcg atctcggaaa taacccgctc aggttctgcc
    19921 tgcggcaaat caattttatt cagtactggc aatacggtaa gagactgatc aattgctgta
    19981 taacaaactg ctaaagtttg tgcttctact ccctgtgctg catcaacaac cagaatagcg
    20041 ccttcacaag cggcaagaga acgagatacc tcgtaactaa aatcaacatg ccctggggta
    20101 tcgataaaat tcaataaata ggtctttcca tctttagctg tgtaattcaa ggaaacgcat
    20161 tgcgctttga tggtaatacc tcgctcacgt tcaatatcca tagaatcaag aacttgcgag
    20221 ctcatttcgc gctcagtcaa cccgccacaa atttggataa atcgatcagc caacgtagac
    20281 ttgccatgat caatatgggc aattattgaa aaatttcgaa ttcggtttaa atctttcaac
    20341 tgttctaacc ttcttgcaaa tagcataagt ctatcttaaa tgttcgcatg cagaaagtag
    20401 gcaaagcaaa ttcagtcgtt tttatattct taagagaatg aaaattgctt attcatttac
    20461 caatttcatg cactgtattt aagataaaca aaagtcaaaa gtttgctatg aggctttatt
    20521 tttactaaaa ttcgtgcttt gcaacgatac agggtacatg atgcgacaaa tcatctggct
    20581 aggtttcatt tcactcttcc ttttatcttt cactacctat tccgacacaa cttttacaaa
    20641 acgtaaagac gtacaatcgt ttattaacat gatggtaacg aattatcact ttaatcgtaa
    20701 tcagttaact acagtaatga accaagttaa acttcaacca caagtcatcg aatctatgga
    20761 aaagccctat gaaaagaaaa actgggatgt ctaccgagaa ctgtttctta ctcagaaaag
    20821 attaaaaagc ggattgcaat tttgggctga aaatcaaaaa acattggaaa aaattcagag
    20881 aagatatggg gtgtctccgg agatcattat agctatttta ggagtagaga caatgtatgg
    20941 tgagcgtcag ggtgaataca gagtcttgga tactctggct acactggctt tcaactaccc
    21001 caaacgttct gcattcttta ctaaagaatt aaaggagttt ttcctcctgt gccgagaaca
    21061 gcacgtatcc ccaactcaat acaaagggtc ttacgcaggt gccataggaa aaccccaatt
    21121 tatgccaagc agttatcgat attatgcagt ggactttaat aacaatggtc gaagagattt
    21181 agtcaatgac aatggtgact ctattgccag catcgccaat tatttgcata aacatggttg
    21241 gaacttaaat caaggcatcg ctcaaccggc caaaatctcc ggatggcgat acaaacaact
    21301 tcgcatgaac cctaaaacag caaattaccg atactcacaa cttataagct atggagtaaa
    21361 accaattaca gcgtcacaaa atcatcctcc tcgtgcagga gtcattgagt tagttacaag
    21421 gaagggtaaa gaatactgga ttgcctaccc caattttttt gtaattaccc gctataattc
    21481 gagccctcaa tatgctctgg cggtttactt attatcccag caattgaagc aacattggta
    21541 tactagtgcc accaaaaagc acagagctta tgtttaatgc cagctggaaa taataataat
    21601 tactggtata tactgaaaaa ttgacagcca taacaataca gagtttgact tatggataag
    21661 tgttttattt ttagcaaaca tctcgatgat aatggttgtg tatgcctgaa aatatctgac
    21721 ttgggtgagc tcattgctcc ccctgaattt cggaatttct cgcaaataaa agatttacaa
    21781 aaagaatcca gtacaattat tgtagaaacc tgtaccagag caatacttct tggtcttgca
    21841 attccatggc tacctgaaag aaaagccaga attgcaattc cctatgcact agaggataaa
    21901 gtaactcaac cagtcgaaga acttcatttt gcctttgata aattttatta tcagaataac
    21961 caatatctcg ttgttgtaat tgataaacaa cgaatgaaaa agttaattca agtttttgat
    22021 gagcatggga tagagtttga tgcagtaact cttgactggt ttgcgctagc atctcaagaa
    22081 ttgatcatta gtgaatccga gctattgatc aataacgaag attttaaagg tgtcctctca
    22141 ggtgatttgg ctcaaactta cctgaaaaat catccacaca ctcccgctca tctgtttcaa
    22201 gacagcaaaa tacctgtcgc ctctccagca agccaaaatg aagaacactc ttacacctgg
    22261 attagtaaaa gattgataaa atcaaaacca ctcaatcttt gtcaaggtga aatacaacat
    22321 ggaaaaacaa ccgattgggt taaaaaagga tacctattgg ttggcggatt atgcggcttc
    22381 tggttaattt ctattctttt tgttaattgg ctttctttgc attggctcaa tagacaaata
    22441 gatgaaattg accggcaaac cgcggttatt tatcgcgagt tttttcctga tgccaaacag
    22501 gtcatcagtc ctaagtttcg aataagtcag cttcttgggg ggagtactgc agaaaatcaa
    22561 actcgatttt ggtttatatt aaatcaatta tccaaagcca taaaagatag tggtatcaca
    22621 gtagagcaat taaggtatca aaacaaaaca atatcaatca cattggtaag ttctgatttt
    22681 gaaagcttac aaaaaattga aaataaacta aaacaatcac aactcaaagt gaaacaaact
    22741 caagcctcaa cgaaagatca acaagtagtt gcaactctgg agttaacgtg aaaacctatt
    22801 taaacacgct gaatgatcga gaaaagtgga tggttatagt agctggtgta tcacttttta
    22861 tttatggcta ctatttatta ctttacaccc cgctgagcaa ccaggtaaat caaaaatcca
    22921 ctcaattaat tgaaaaaact gaaacattgg aatggatgaa gcaagtaaga atgcaaaaac
    22981 gctctgccaa aagaaaggaa tctgtagata acagccaatt gttaactatt cttgcttccc
    23041 aacttaaaaa taataaaaca ctcaaattcc cctatcaatt acaacaaaca ggctcggggg
    23101 atgttcaatt aacctttgac gcagtccctt ttcaaaactt tatacaatgg cttgcaaaaa
    23161 tcaacgaagt ctattctatc aatataaaac aatttgatgt ggagaaaacc tcaacaccgg
    23221 gagtaacccg actgatgatc atactctcta caagttagaa ttgctattat gtattagtca
    23281 agactataag cctgacctta gaataataaa atacggaata tcaaagtatt ttttttaaat
    23341 acatatagtt tctcaagata acctattcat tcaaatttcc ttgaacactc ccccaatctt
    23401 taattccagc accctgttta atttcaggag gtaatgattc caatgctttt tgagctgctt
    23461 cttgactatc ataagtacca tataaaccct tataatgggt tttgccattt tgataatact
    23521 tcacttgagc cattctatca tttttaggtg ctttatagag tttttgggcc acataagcag
    23581 ccttttcgtc atcggcaatc tgaatcgtat aaccttgagg attttggctg tttacccaat
    23641 ttctatctct gtccttaaag gatacaggag aatgatactc accaacatga tatgaatcag
    23701 gaaccacaac actttgacca gattgattgc cataattgta atcacgagca cgataatctg
    23761 tttgaggata cagctgacca taatcatagg tataaatttg atagcgggta gtatatgtat
    23821 cctcataaac catacaagaa gaaatattca ttacgccgat acctagaatg atgtacctga
    23881 tgctaatacc catcataaca ccccctgatt ttaactttta cttttactct tatttgttgt
    23941 tatctacaaa tacaaggaaa actttaggaa aatgttttat gaattgattc agaattattg
    24001 gatttctatt caagtcatcg tccgaaaaaa tttcccttat tctgcgcgca tcaaaaaaat
    24061 aaatgccatt ataaacaatc gtaattttcc agagactatg caaagactct attgtttccc
    24121 aacatggtat agaataaagt aattggtttg tggaataagg gcaaaagata tgtggactca
    24181 tagacgttct ggtcaaacca atcaaagagg ctctcaagat catagagatc cctatgagcg
    24241 agataggaca agagtaattc actgtccagc atttagaaga ctacaaagaa aaacacaaat
    24301 tcttggaaca gatgagggcg attttcatag aacccgtcta acccactccc tggaagtaga
    24361 ttctataggt agaagtatcg ttcgcaactt gatgaccaat caggaacacc agttgttatt
    24421 gtccagcctt ttgccaaatg atgacctcat cagcgtcatt tgtttgcttc atgatattgg
    24481 ccatccgccc ttcggacatg gaggagaagt cgcattgaat tatatgatgc gacattatgg
    24541 aggatttgaa ggcaatggac aaactcttcg cttactgaca aaagttgaaa atagttacgg
    24601 tggctttggc ctggatctaa ctcgtcgtgc gttgctgggt attttaaaat acccggtaaa
    24661 acgagctaat gtggtagctc ccaaacaacc acctattcat gaatcgattc ataaaacaat
    24721 caaaatcaat gactggctgc ctcctaaagc ttattttgat tgcgagcagc cagaagtcga
    24781 ttggttatta tcgcctttat cagataatga cagagaatta tttcagtctc tctccgtaaa
    24841 gccccatggc acccaaacag gtaaagcagc ctatcataat tttgattgct ctattatgga
    24901 cacagcggac gatattgcct atggagtcca tgacctggaa gatgcaattc acttaagatt
    24961 aattaaccgt tcacatcttg atactcctga gttcaggcaa ttaatcggtg aaacaaccct
    25021 ctcgctacat caggagcgtt tgcttaattc tctattttct ccagaaatct gtttaagaaa
    25081 acaagctatc ggagaaatgg tcaattattt tattacctct actcaaatca ttgtaaccaa
    25141 tgaagaattt gaaaacagtt tattaaaaca taatattgca cttatcccag aagcccatgc
    25201 cctcctccgc tatttaatgc aatgtattta taataatgtc attgactccc aagaagcacg
    25261 tacctttgag tatggaggtc aaacggtagt actccggcta tttgatgcca tcagctccaa
    25321 tccagccagc ttgcttgata ataaaaatcg agtgttattt tcgcaagctc cagatgaggt
    25381 cgctgcttat agagtcgttt gtgattattt agcaaacatg actgacgagt atgcttatcg
    25441 aatgcatgag agattattcg gatttaatac aagaacaatt tttgaaaggc tttagataac
    25501 aagaaagacc ttaaaacaat agccattcaa
//
LOCUS       DACTJE010000026        25432 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.026, whole genome shotgun sequence.
ACCESSION   DACTJE010000026 DACTJE010000000
VERSION     DACTJE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25432
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.026"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(260..1117)
                     /gene="queF"
                     /locus_tag="I9760_000840"
     CDS             complement(260..1117)
                     /gene="queF"
                     /locus_tag="I9760_000840"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAT5042620.1"
                     /translation="MNKELESIYQNIANQSELGQTANYDSYYNPKRLYPIPRAPKRQE
                     INLDPNSTTFYGFDCWNHYEVSWLNSKGKPVVAMAVISYDCHSPCIIESKSLKLYFNS
                     LNNSTFPDVETVVQTISKDLSYCIGSEVAVNVYPLSEIASQTIYAAFDGVCLDKLDIE
                     CSVYHVMPDFLSTSSELVEEVLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLK
                     YLISFRNHNEFHEQCIERIFADIMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCAFD
                     GQNIRLIRQ"
     gene            1262..1663
                     /locus_tag="I9760_000841"
     CDS             1262..1663
                     /locus_tag="I9760_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAT5042621.1"
                     /translation="MINQQIEVFLKSPAFAVVGASSNREKYGNKVLRCYLRNGKKVYP
                     VNPHETLIESLPVIHSVNDLPDDVHSISIITPPSITEEVVKEAVKKGIQHIWMQPGAE
                     SDSAIQYGLDHKLNVISGGPCILVVMGYEEN"
     gene            complement(1728..2849)
                     /locus_tag="I9760_000842"
     CDS             complement(1728..2849)
                     /locus_tag="I9760_000842"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAT5042622.1"
                     /translation="MPLQPFKTSNLLTMGVELELQLISLSNFDLTAASPDILELLGRS
                     SFPGSFTPEITESMLEIATDVHEEYDQLLKQLFHIRDALVTVGDRLNIGICGGGTHPF
                     QMWSDQRIFNKTRFIEVSELYGYLTKQFTIFGQHIHIGCEDGNQALFLLHSLNRYIPH
                     FIALSASSPFVQSKDTLYNSARLNSVFAFPLSGRAPFVLNWDEFSLGYFEKMEHTGIV
                     KSMKDFYWDLRPKPEFGTIEMRVCDSPLTVERAAALACYMQALCSYLLENKEPLPHED
                     DYLVYNYNRFQACRFGLDGTLVHPKTYEQILLREDILTTLRRLKPYANQLNSTMALEH
                     IYEITHKGSDASFLREKYAEHRTLESVVNESLKQFRRSK"
     gene            complement(2851..4038)
                     /locus_tag="I9760_000843"
     CDS             complement(2851..4038)
                     /locus_tag="I9760_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAT5042623.1"
                     /translation="MIDVGKIITDLAWPISLLIAWFAGELGYRWAKLPHISIYAIVGF
                     SLASTQIGLLPSIQPPSILLLANIGFGLILFESGYRINLRWLSYNPWVAASSITEATL
                     TFCLVYILVKSFGLQTSTCLLLASLSMATSPATVVRVINEQRSSGQVTERVLHLSVIN
                     CVLAVFLFKVIIGLVVFRTSGNLWEAAYASLTVTAISTIWGIIFGVIMPILLKTVKST
                     CQDNTLAFACAVILLVILTHNLKLSPVLATLTFGLVSRHGRMVFSPSQPGFGTLGDLL
                     SVLLFVFIAATIEWQRVVTGVWIGLSIITVRFVAKIIGISLFAHISGISLKKGILTGL
                     AMTPISVFVILILEQSRYIGINLVDTLAPLAMVALTLEILGPIMVQRALIKANEISAI
                     KEF"
     gene            4368..4970
                     /gene="def"
                     /locus_tag="I9760_000844"
     CDS             4368..4970
                     /gene="def"
                     /locus_tag="I9760_000844"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAT5042624.1"
                     /translation="MALEIITIEKKENYHMLKTRAENIQFPLNREDKDLIEAMKKKLY
                     ALGGVGLAAPQVNSAKQIIAVYIPEEAQLLRDNAKIFPMHILINPNYEPLPHTRIISD
                     FEGCYSVSSKAGKVPRYDEIQLKYYDEEGNVHQQIENGFYARVLQHEIDHLNGVLITD
                     RLTPDCVQGTIEEMMTIRRAELPVEKRKLFDQIMAKKLKK"
     gene            complement(5006..5410)
                     /locus_tag="I9760_000845"
     CDS             complement(5006..5410)
                     /locus_tag="I9760_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HAT5042625.1"
                     /translation="MHKLIELQGVGPKYAEMLKRVGIESQEDLLRVCSGRRGRFRVAE
                     QTGISHKLILKWTNQADLARVNGINEEYAELLEQAGIDSIPELAHCNPEQLYSSLKEK
                     NEQCHLAQHVPGLSQVVTWVEQAKKLPRAVYH"
     gene            5598..5942
                     /locus_tag="I9760_000846"
     CDS             5598..5942
                     /locus_tag="I9760_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042626.1"
                     /translation="MRKHKNKMVRIFLLALGLFSSTTINAWYGSGITIGVARANVHYY
                     GDDIRVYDPWGYGYYDRNYYYNGVRNIIVPNVIVPNVVINVPVQRYYVPSCEEMEVCD
                     PYGQCWLEQFCR"
     gene            6143..7189
                     /locus_tag="I9760_000847"
     CDS             6143..7189
                     /locus_tag="I9760_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HAT5042627.1"
                     /translation="MSETIGYAAHKAGAPLKPFTFERRPVGAHDVAIDILFCGICHSD
                     LHQVNNDWAGAFYPMVPGHEIIGRVKEVGHQVRRFKAGDLVGVGCMVDSCRQCTYCHQ
                     NEEQYCKSGATFTYNSQDNHLGGVTYGGYSKHIVVTEDFVLKIDESLDPAAAAPLLCA
                     GITTYSPLKRAKVGKMSKVGIVGLGGLGHMAVKLARAMGAYVTVFTTSINKIKAGKDL
                     GAHDVVLSTDSNVMQTLANQYDFILDTVSAKHDLGQYLNLLKVDGQLTQVGLPSDPLE
                     VTMFPVILKRLSINGSLIGGIKETQEVLDFCARHGITADIELIKMHEVNSAYERLLKG
                     DVKYRFVIDMSTLD"
     gene            complement(7243..8562)
                     /locus_tag="I9760_000848"
     CDS             complement(7243..8562)
                     /locus_tag="I9760_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAT5042628.1"
                     /translation="MYNILLIFIAFLLVLLNAFFVSAEFGMVKLRATRIQAIKENYGL
                     RGRILEQVHQNLDAYLSACQLGITLASLGLGWIGEPAFAELLGPLFIALGISSVHIVT
                     ISSFFIAFFLISFLHIVIGELMPKSIAIRQSELISIWTAVPLYWFYWIMYPAIWLLNT
                     CSNFFLKLSGMDIKNEYEGFYSSEELKLILSGQHLQSELKEDEREILEHTLDLAELNV
                     TEVMRPEDEMIIIDINEPFETIKDKILKYRYSRYPVYDSGTKEMIGVIHVKDLFADLL
                     LQKNINTLKPYVRPVLKVSHKLPAMDLLRKFRAGMPHFALVYKHKSSLLGFVTLDNLL
                     HILIGRIKDEFHKTHEDWVKNSDGTITTKGDCSLYSLERALDCDITLSPDEEELNTLY
                     GLIITRHGKLPNEGEIIQFVEFDALIEKVQDSFITKIKVFPKIKNQE"
     gene            9216..10511
                     /locus_tag="I9760_000849"
     CDS             9216..10511
                     /locus_tag="I9760_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042629.1"
                     /translation="MNQNSFKPKNNFVSLVMWALPLSFFTFQFLLRLWPGLTMQDIMA
                     QFSIDAASFGLIAAFYYYGYAGMQIPVALLLDKFGARFVVFAFAMLCGIGTLVFIGTN
                     NFYLALSGRFLIGVGSAAGFLGVSKVVSEWFSHSQYSRMIGFSFSVGLLGAIYGGKPL
                     AHLIQNFSGQQVGLALAVFSILLGCCALLILRSPPNNTHSVTKTMNLSHCSSLFSSWI
                     IWALALANLLMVGALEGFADVWGVPYLIAKYALNKTDAAFLISFIFMGMIFGGPLLAF
                     LSQKLGNYFVIALCGAGLAFLFLILLYTSTLHWWQLGTLFFAIGLLCCYQVIVFAAGS
                     TLVPYEGLGVTIAFLNCINMLGGSFFHTLIGKLMNSSWRGQMSANGLKLYEIESYQWA
                     LSLIPFCALCGSLLIVLIHIKTRAYAAPGFIKKTSLTGV"
     gene            10514..11158
                     /locus_tag="I9760_000850"
     CDS             10514..11158
                     /locus_tag="I9760_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HAT5042630.1"
                     /translation="MEKIFWSNPYQHTLDTKIISINGNEILLEKTIAFSFSGGQESDR
                     AFINGLEVLSSRIDGNLIYYTLPDDHNLAPNDVITMTIDWPRRHRLMRLHFAAELILE
                     LVTRTSMLEKVGAHISESKARIDFKANFNISSIFSQLLDEYNAIIRQDLVIYKGYHDV
                     TNQRRFWKIDGFAEVPCGGTHVHSTSEVGFIRLKRSHPGKSIERIEITLMDDSR"
     gene            11405..12319
                     /locus_tag="I9760_000851"
     CDS             11405..12319
                     /locus_tag="I9760_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT5042631.1"
                     /translation="MELPYHFSTAYVSKPEFIEALSEELGEVSNKIGSLVFSPHQKLD
                     VCFALDVWLEPEIVTFQSISEAAKILRQAGKFWYLHPVTEIRRSRLIEEQLRKCPSLM
                     SHFPVTSDIPPIGVFSLLDRNTLLFSAKRLKKWPDGQCYFIEDKVNPPNRAYLKLWEA
                     LFLLNKYPLPGETVLDLGASPGGWTYVMQSLGTQVTSVDKAELDTKIMALPRVSFLKQ
                     SAFALDPEQLTESYDWVLSDVACYPDRAYTLIKKWIASKKAKQLIFTIKLQGQTDLNT
                     IKRFQSIPNSKVINLFYNKHEVTFFYPV"
     gene            complement(12392..13345)
                     /gene="epmA"
                     /locus_tag="I9760_000852"
     CDS             complement(12392..13345)
                     /gene="epmA"
                     /locus_tag="I9760_000852"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAT5042632.1"
                     /translation="MSDNSWLPSASIQLLRARAELIGKIRQFFTERQYLEVETPVMAR
                     HGVTDVYLSNIKAIFRGETCYLQTSPEYHMKRLLAAGSGPIFQLARVFRDDELGRWHN
                     PEFTLLEWYQLHIDHHVLMDEVDLLLQIILKSRPMIRKTYQQAFEEACGIDPFSTSIE
                     QFNKVLAQHDLDGVLSPNEQDRDQYLFLLMSHVVEPFLAEESTPVAVYHFPASQAALA
                     QVKEGLAERFEVYYQGVELANGFHELTDVEAQKKRFMLDQQHRKQKGLTAMEADAYLL
                     KALEYGLPPCSGVALGVDRLLALALKQPSIEKILSFDFSRS"
     gene            complement(13348..13683)
                     /locus_tag="I9760_000853"
     CDS             complement(13348..13683)
                     /locus_tag="I9760_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HAT5042633.1"
                     /translation="MFGFKKKQSNESIKEGVITALRGVFDPEIPVNIYDLGLIYDIAV
                     NDEGHVHIQMTLTTPGCPVAQTFPGTVEQAVNQVEGVSDCTVELVWEPPWSQDRMTEA
                     ARLELGIFY"
     gene            complement(13755..14204)
                     /locus_tag="I9760_000854"
     CDS             complement(13755..14204)
                     /locus_tag="I9760_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAT5042634.1"
                     /translation="MSMELRELYQEIIIDHNRNPRNHHAMEDATTEAKGFNPLCGDKL
                     TVYLKLQGDLIRDVSFIGCGCAISQASASLMTDALKGKSIKEAHELFHRVHRMLTQEE
                     EDSLVSMDKLTVLAGVKAFPARVKCATLAWHTLEAALNKETEVVKTE"
     gene            complement(14201..15424)
                     /gene="sufS"
                     /locus_tag="I9760_000855"
     CDS             complement(14201..15424)
                     /gene="sufS"
                     /locus_tag="I9760_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HAT5042635.1"
                     /translation="MTSFDVNKIRKDFPVLHQKINEYDLVYFDNAATTQKPKAVIDAI
                     AQFYEKDNSNVHRGVHALSVRATEMYEAARAKVKRFINARSPRECIFVRGTTEAINLV
                     AQSLVAPRILPDEEILITHMEHHSNIVPWQMVCKKMGCKLQVAPISLNGEVILEEFER
                     KLNENTKMVAINYASNSLGTINPVKTMIKMAHEVGAKVLLDGAQATAHLIVDVQDLDC
                     DFYAFSGHKMYGPTGIGVLWGKEELLNSMTPYQGGGEMINSVSFEATEYAAIPHKFEA
                     GTPNIAGAIGLAAAIDYIWSLDLDAIAEYETQLLNYATKAIEAVKGYNIIGTAANKVP
                     IISFVHGKIHAHDIGTILDSEGIAIRSGHHCTMPLMDFYDVAATSRISMSFYNTFKEI
                     DYCMEALQRVKEVFA"
     gene            complement(15435..16721)
                     /gene="sufD"
                     /locus_tag="I9760_000856"
     CDS             complement(15435..16721)
                     /gene="sufD"
                     /locus_tag="I9760_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAT5042636.1"
                     /translation="MSDILDYYQQQAQLGVSQIPWLAQLQSKALTDLKRRGFPTRYDE
                     EWKYTLVDSLLQQHFVPPDPTVKPALKTAFDDSPVKNLFLIQNGFISQEQEWIKNLPQ
                     GVLILPLSLALIHYADLVKPHLGKIMEQEHGFHYLNTAMLQCGVFIYIPEGICLEEPI
                     VLKHIQDQTNQAMYLRHLILMEKESQATLIEEYHGEENICYFTNSITEVFLGAGAKLN
                     HYKIQKESKSAYHLGHLSVRQSAASQFSSHSLSLGGKLVRSDISVSMQEEQAQCLMNG
                     IYAPGDGQHIDHHTVVHHLVPNCRSDQDYKGILKGHSRAVFNGKVIVAKNAQHTDAKQ
                     QNKNLLLSANAEIDTKPQLEIFADDVLCSHGATVGQLDEEAIFYLATRGISRDEASHY
                     LIQAFANDNLRLIPNRDLADWMGNLLTQQLGYANEY"
     gene            complement(16718..17470)
                     /gene="sufC"
                     /locus_tag="I9760_000857"
     CDS             complement(16718..17470)
                     /gene="sufC"
                     /locus_tag="I9760_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAT5042637.1"
                     /translation="MLNIKELNVAVNGQSILKGINLEVKPGEVHAIMGPNGSGKSTLS
                     KVLAGHPSYEVTRGDISYLGQDLLPMSPEERARAGIFMSFQYPVEIPGVTNINFLKAS
                     VNAVRKGQGKNTLDAIEFLSFIREKCQLLDMDESFLYRSINEGFSGGEKKRNEILQMA
                     ALEPKLAILDETDSGLDIDALRIISQGVNAMRSPERSIILVTHYQRLLDYIEPDFIHV
                     LANGRIITSGDKSLALELEKKGYSWLEEVEQL"
     gene            complement(17472..18920)
                     /gene="sufB"
                     /locus_tag="I9760_000858"
     CDS             complement(17472..18920)
                     /gene="sufB"
                     /locus_tag="I9760_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAT5042638.1"
                     /translation="MAKSSEQLNSLLDREYQHGFITDIEVETFPPGLDEDVIRRLSAI
                     KGEPEFLLEWRLKAFRHWLTMPHPEWSSVHYPPIDYQSVSYYSAPKSKKDAPKSLDEV
                     DPELLKTYEKLGIPLREQEMLAGVAVDAVFDSVSVATTFKAKLAEKGVIFCPLSEAVH
                     QYPDLVKKYLGSVVPYRDNFYAALNSAVFSDGSFVYVPNGVRCPMELSTYFRINAAST
                     GQFERTLIIADVDSYVSYLEGCTAPMRDENQLHAAVVELIALDGAQIKYSTVQNWYPG
                     DKEGKGGIYNFVTKRGACRGKKSKISWTQIETGSAITWKYPSVILQGDDSVGEFYSVA
                     LTNNFQQADTGTKMIHLGKNTRSTIISKGISAGHAHNAYRGLVRIAPTATNARNYTQC
                     DSMLMGSQCSAHTFPYIEVKNPTAQVEHEATTSKISEEQLFYCQQRGIDTEDAVSMIV
                     NGFCKQVLKELPMEFAVEATKLLGISLEGAVG"
     gene            complement(18913..19374)
                     /locus_tag="I9760_000859"
     CDS             complement(18913..19374)
                     /locus_tag="I9760_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HAT5042639.1"
                     /translation="MLRVSKLADYGTVVMVYLAKHSQELCNARDIALHTHLTVPTVSK
                     ILKRLTSAGLLTSVRGVSGGYRLQRPAAEISVSQVIFALEEHRGFTECSMQPNDCFLQ
                     GVCTIQGNWRLISHAIETALDSVSLEALAKPTLPTRDVERVRQLASGVNRG"
     gene            19815..21557
                     /gene="phaC"
                     /locus_tag="I9760_000860"
     CDS             19815..21557
                     /gene="phaC"
                     /locus_tag="I9760_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HAT5042640.1"
                     /translation="MTHDTELSELMQAVAKKSLQIMTDFKEKPIPLSSLVSQYIDLTE
                     HFQNLIAVILKNPEKVWQMQLNYLEDALSLAQAQFNYWLEGKPLPINDQRFNGEDWIN
                     NPFFNLLSQHYLLANEHMNSLLENMEYGDENLAKRVRFFTRQYLDALSPANFIHTNPQ
                     LMAETLQSHGKNLLRGLHNLLSDVEAGSSRLIIKMTDTEAFKIGENLATTPGKVIFRN
                     SMMELIQYCPRTTKVKSIPLLIVPPWINKYYILDLSPHNSLIRWLVEQGITVFIISWV
                     NPDKTYANKSFYDYLNEGPREAISVIQKQLRVKQVNTLGFCIGGTLLASLLAYNKATK
                     DLSIRSATFLAAMIDFSDPGDIAVFIDEQQINKLEEEMKSKGYLAGKFMASSFNSLRA
                     NDLIWSFFIKNYLRGKNPVPFDILYWNADSTNMPATMHSQYLRWMYLHNDLIKPGKIR
                     LNHTPIDVTNIDIPTFFLSTQKDHIAPWKTTYKGFELMKGPKRFVLGGSGHIAGIINP
                     PTQQKYGYRTNSSMDLSAEQWFEKSKEHSGSWWPEWLNWLKLHSGRLTNSPDINHLPF
                     APIMDAPGSYVLKK"
     gene            21606..21989
                     /locus_tag="I9760_000861"
     CDS             21606..21989
                     /locus_tag="I9760_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042641.1"
                     /translation="MRKKNRILLASLFLLGSLNAFASQDLTVVNTSDDLGYVTIGSYS
                     CELTSSCAIPPDNSIRINSDIVEAACANTPVDCEIVFGVDSIKARVATGSFSLNEGIQ
                     YIAQSRWLNYSAKRLSKTVAEIYKR"
     gene            complement(22076..22495)
                     /locus_tag="I9760_000862"
     CDS             complement(22076..22495)
                     /locus_tag="I9760_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HAT5042642.1"
                     /translation="MKLIWIVLALILAFVVILYLFPQINFREMPKGLVEGQLPDDKPN
                     WVSSMVNESDSHYIAPLKVESMPQLVACIERKIPELKIQKQEASLLVGYRQSRFFHFV
                     DWLCIHADGNVISSATMGYSDFGKNRELVEKIRSLCL"
     gene            complement(22496..24697)
                     /locus_tag="I9760_000863"
     CDS             complement(22496..24697)
                     /locus_tag="I9760_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HAT5042643.1"
                     /translation="MMTKTNEENLTWKEKQKQRARAWIKILLEKWFRIEVKGQYQANS
                     HSVIIANRASIIDVLLLSVFLPERLTLALHPDMFKKIWVKTLLFFADVIVVDPGSAQA
                     TRVLIRAIRAGKRCLLFPQGLLRQQEDSLKVFEGPGLILQKVGAEVIPVRIEGAEHSI
                     FSNSKNKHRIRIFPKITLHILPAQKLIQDENTGVDREGISLRLFQLISDLSFANSFKP
                     YVLFSALIEGVSIGAKNKTKIEDNNRIPLTYRQFLARCFILGRQIKKQTAIGETVGVM
                     MPTTIAGMVTFFALQAYRRIPAMLNFSMGFYNLYSACNTARIKSIYTARQFIETARLE
                     PLVEELQQAGIRIHYLEDFKSTIHLGNKLSGIFKGFFPTLSYRFLGDKISPDTTGIIL
                     FTSGSEGKPKGVALSHANILANCWQMTSRVDFTPRDVLFNSLPIFHCFGLTAGSVLPL
                     VNGLNCFFYPSPLHYKVIPGLVYQTGATILFGTDTFLTGYARAAGKHDFNSVRYIFAG
                     AEKVKPETIRYWSEAFGARIYEGYGATEASPVISLNCPLASVPGSVGMILPFMDFKVE
                     PVEGIVQGGRLKLCGPNIMLGYLDEDKPGVIIEPEDGWHDTGDIVTVNADGFITIAGR
                     AKRFAKIAGEMVSLTAVEGIAASIWPELLHAAVVQKSPRKGEQIFLFTEAEYADKVSF
                     IKKVKERGQSELLVPHMIYPACQIPVLPSGKIDYITLEKQFLEQKETMETV"
     gene            24852..>25432
                     /locus_tag="I9760_000864"
     CDS             24852..>25432
                     /locus_tag="I9760_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAT5042644.1"
                     /translation="MRVLNSCNKVRGIFTLTQKTIKTFCLILAILVLIIFAVVFHNHS
                     LEIIDYIDDLGWLAPVLFLITYCLATLLLLPTMVLTLAGGAVFGPVFGTLLNLLGATS
                     GAAFAFLITRHLVYDWFSTKKGEKLNKLIAGVDEKGWVFVAFLRLFPIVPFNLVNYGL
                     GVTGISFRLYLLTTFIFLIPAEIIYTYFGYVGM"
BASE COUNT         7820 a         5290 c         4679 g         7643 t
ORIGIN      
        1 ttgaccttgg agttttcgta agtcctgtca atcaaaataa actccaaaac caagttttta
       61 ttctttaatc tatggatttc agcgatggac caatcgaaca gcgatgagta aatctgtgga
      121 attaagttta agatttatgc ctgaattggt acaaatttcc aggcaatctt agcataggaa
      181 agatgtaatg tattgaaaat taattattaa attcccaggc gggattaaaa tttatccctt
      241 aggggataag taccttcttt tattggcgta ttaaacgaat attttgccca tcaaaagcac
      301 aaggttctgt tgacctgatt gggttaatat ccagacctcc ccttcttgta tatcgtccat
      361 aaacagtcaa tgactcaggt tggcaaaatc gcataatgtc tgcaaatatc cgttcaatgc
      421 actgctcatg aaattcatta tgatttctaa atgaaatcag gtatttaagc aacccttcat
      481 gatttatctt tttaccttta taaataattt gaacgcttcc ccaatccggt tggttggtaa
      541 ccaggcagtt tgatttaagt aaatcagaat acaatacctc ttcaactaat tcagacgagg
      601 tcgaaagaaa atcaggcatt acatgatata cagagcattc aatatcaagc ttatcaaggc
      661 aaacaccatc gaatgcagca tagatagttt gactagcgat ttccgacaag gggtacacgt
      721 taaccgccac ttcagaacca atgcagtaac ttaaatcttt gctgatagtt tgcacaacgg
      781 tttccacatc agggaaagta ctattattca acgagttaaa atagagtttt aatgacttgg
      841 attcaataat acacggggaa tgacaatcat aagaaataac agccatcgca acaacaggtt
      901 tacctttgga gtttaaccag gagacctcat aatgattcca gcaatcaaaa ccatagaaag
      961 tggtggagtt gggatccaaa ttaatttcct ggcgttttgg ggctctgggg ataggataca
     1021 gccttttagg attatagtag ctgtcataat ttgcagtttg ccccagctcc gattgatttg
     1081 ctatattctg ataaatactt tcaagttcct tattcattat ggatgccttt tgtaactcta
     1141 aaaaacaaag tgattataat ctatcatgtt acctaagggt caattttttc ttaaaaaaaa
     1201 ggaaatagcc tatatttaat taaagatact gaaattagca tctaaaggag tgcatttacc
     1261 catgattaat caacagatag aagtattttt gaaatctcct gcttttgccg tagttggcgc
     1321 ctcttccaat agggaaaaat acggcaacaa agtattgcga tgctatcttc ggaatggcaa
     1381 aaaagtctat cctgtaaatc cacatgaaac gctgattgaa agcttgcctg ttattcattc
     1441 agtaaacgat ttacctgatg atgtacacag tatttctatt atcacccctc catccattac
     1501 tgaagaagtc gtcaaggaag cagtcaaaaa aggaatccaa catatatgga tgcagcctgg
     1561 agcagagagt gactcggcca ttcaatatgg cttggatcac aagcttaatg tcatttctgg
     1621 gggaccttgt atattggttg tcatggggta tgaagagaat taaggaaata caaaaagcta
     1681 ttttgtagtc caaaatagct tttttgattt tgtataatcc cagccgatta tttagaacgc
     1741 ctgaattgct ttaatgattc attgaccaca ctttcaagag ttctatgttc tgcatatttt
     1801 tctcttaaaa agctggcatc actcccttta tgagttattt cataaatatg ctccaaagcc
     1861 atagtactat ttagctgatt agcataaggc tttaatcggc gaagtgttgt taaaatatct
     1921 tctcgaagca aaatttgctc atatgttttg ggatgcacca gagtgccatc cagaccaaat
     1981 cggcatgctt gaaaacgatt gtaattgtac acaagataat catcttcgtg aggcagaggt
     2041 tctttatttt ccaataaata actgcaaagc gcttgcatat aacatgctaa agcggctgct
     2101 cgctcaaccg taaggggact atcacacacc cgcatttcaa ttgtcccaaa ttcaggttta
     2161 ggtcttaaat cccaataaaa atccttcata cttttcacta tgccggtatg ctccattttt
     2221 tcgaaataac cgagtgagaa ttcatcccag tttaatacaa agggagccct tccacttaat
     2281 gggaaggcaa ataccgaatt caatcgagca gagttataca aggtatcctt actctgaacg
     2341 aagggagatg aagcagacaa tgcaataaaa tgagggatat aacgatttaa agaatgaagt
     2401 aaaaaaagcg cttgattacc gtcctcacaa ccaatatgta tatgttgccc aaaaatagta
     2461 aattgcttgg ttagatagcc atataattca gacacctcta taaacctggt tttattaaaa
     2521 attcgctgat cagaccacat ttgaaatgga tgggttcccc cgccacaaat accaatattt
     2581 aatcggtctc caactgtcac cagggcatcg cgtatatgaa acagttgttt cagtagttga
     2641 tcatattctt catgcacatc tgtcgctatt tctaacatac tctctgtgat ttctggcgta
     2701 aaactgccgg gaaaagacga tcgtcccaag agctccaata tatcagggct agcggcagtc
     2761 aagtcaaaat tacttaagct gattaattgt aattccagct caacccccat agtcagtaaa
     2821 ttagatgttt tgaatggttg taaaggcatt tcaaaattcc ttgattgcag agatctcgtt
     2881 cgctttaatt aaagcacgtt gaaccattat tggacccaat atttccaaag tcagagcaac
     2941 catagccagc ggggccagtg tatctaccaa attaatacca atatatcggc tctgttctaa
     3001 aatcaaaatg acaaaaacag agatgggggt catagccaaa ccagtcaaga tacctttttt
     3061 aagcgaaatc ccactaatat gggcaaataa actaatcccg ataattttag ctacgaacct
     3121 tacagttata attgataatc ctatccatac accagttact accctttgcc attctatggt
     3181 tgctgcaata aatacaaaca aaagtaccga taacaaatca cctaaggtgc caaatcctgg
     3241 ctgtgaagga gaaaaaacca ttcgcccatg acgggaaacc aacccaaagg ttaatgtggc
     3301 taaaaccggg gacaatttta aattatgggt taaaataacc agcagtatca cagcgcaggc
     3361 aaatgccaac gtattgtctt gacaggtgct tttaactgtt ttaagcaaaa tgggcattat
     3421 cactccaaaa ataatccccc aaatagttga aatggctgtc accgttaagc tggcataagc
     3481 tgcttcccaa agatttcctg aagttctgaa aacaaccaag ccaatgatga ctttgaataa
     3541 aaatactgcc agcacacaat tgatcacgga gagatgtaaa acacgttctg tcacttggcc
     3601 tgaactacgt tgttcattaa tcaccctgac tacagtggct ggtgaagtcg ccatggacaa
     3661 cgaagccaat aaaaggcagg tcgaagtttg taacccaaag gatttcacta aaatataaac
     3721 caggcaaaag gttaaagtcg cttcggtaat actggatgct gcaacccagg ggttataact
     3781 taaccagcgt aaattaattc tatagcctga ttcaaacaag attagcccaa aaccaatatt
     3841 ggctaaaagt aaaatagatg gtggttgaat agacggtaat aatcctattt gagttgatgc
     3901 tagtgaaaat cctacaatcg catagatact gatatgaggc aattttgccc atcgatatcc
     3961 aagttcccct gcaaaccaag ctatcaataa actgataggc caagccaagt cggttataat
     4021 ttttccaaca tcaatcatcc atacctccta tgaaaatccg accttacttt ataacacaca
     4081 tcgatttgtt ttgcagaatt gaagtatttt taagacaata taaaataatc aggacttacg
     4141 aaaaatccta aggtcgaggc aaaaaatgtt tttaatgaat gagtttagat aaaataaatg
     4201 gctgaattaa aaactttttt aacaaagaga ttgaggtttt tcgtgagcac tgatagtttt
     4261 ttgtccaatt aggaaaatca ttgctactaa ccacattaac gagaaccaaa aagaaatctc
     4321 aacaacttct tattttaata ggcttcttat tagggatgtc accagtcatg gctttagaaa
     4381 ttattacaat agaaaaaaaa gaaaactacc atatgctaaa aactcgggct gaaaatattc
     4441 aatttccctt gaatcgagaa gataaagatt taatagaagc gatgaaaaaa aaattatacg
     4501 cattgggagg ggttggtttg gcagcaccac aagtcaattc cgccaaacaa attatcgcag
     4561 tttacatccc tgaagaagct cagcttttac gtgataatgc gaaaatcttt ccaatgcaca
     4621 tcctaatcaa tccaaattac gagcccctgc cccacactcg tattattagc gactttgaag
     4681 gttgttattc tgtatccagt aaagcaggga aagtgccaag atatgatgaa attcagctaa
     4741 agtactatga tgaagaaggg aatgttcacc aacaaatcga aaatggcttt tacgcccgtg
     4801 ttttgcaaca tgaaattgat catctgaatg gcgtattaat caccgatcgt ttaactcccg
     4861 attgtgtaca agggactatt gaagaaatga tgactattcg ccgtgctgaa ttgcctgtag
     4921 aaaaacgaaa attatttgat caaatcatgg caaaaaaact taaaaaataa ggtcgagttc
     4981 ccaaattatc ttttatgaat tgacttcaat gataaactgc cctgggtaat ttttttgctt
     5041 gttcaaccca ggtaacaact tgagataatc caggtacatg ttgcgccaaa tgacattgct
     5101 catttttctc ttttaatgag ctataaagtt gttcaggatt gcaatgtgct aattctggga
     5161 ttgagtcaat accagcctgt tccagaagct cagcatattc ttcattaatg ccatttactc
     5221 gcgcaagatc agcttgattg gtccatttca aaatcaattt atgactgatt cccgtttgct
     5281 cggctactct aaaacgcccc cggcgaccac tacaaactct taataaatct tcctgacttt
     5341 caatccctac tcgctttagc atttcagcat attttgggcc tacgccttgc agctcaatta
     5401 acttgtgcat tttatttcca gtcttctact acgatattac atatataaaa ttatagaata
     5461 taatgattaa ttattaacca aaacaaatgt tgaaaaaatt taggcctcac gtattaaaaa
     5521 aaaatcaatt catttggcaa aatgaaacaa tttcattatg ataattactt tcctttcagt
     5581 ccctggtgag gtagaaaatg cgcaagcaca agaacaaaat ggtcaggata tttttattgg
     5641 ctttaggact attttccagc accaccatta atgcttggta tggctcaggg ataaccattg
     5701 gtgttgccag agcgaatgtt cactattatg gagatgatat ccgagtatat gacccgtggg
     5761 gctatggata ctacgacaga aattattact ataatggagt aagaaacatt atcgttccta
     5821 atgtaattgt gccgaatgta gtgatcaatg taccagttca aaggtattat gtaccctcat
     5881 gtgaggaaat ggaagtttgt gatccttatg gacaatgttg gttagagcaa ttttgcagat
     5941 aaattaaaaa ctcaatcaac taatgttttg ggactcagcc cattatggga tgagatatca
     6001 gcaggactta caaaaatccc aagatcaaga caaaaaatgc ttttaatgag ggagtttaga
     6061 taaactaaac aaccgaatta aaaacatttt ctaacaaaga gattgggggt atgtcctgat
     6121 tataattttt taaggacaaa aaatgtcaga aactattggt tatgccgccc acaaagcggg
     6181 agccccttta aaacctttca cttttgaacg ccgccctgtt ggtgcacacg acgtagcaat
     6241 tgatattcta ttttgtggta tctgtcattc cgatttgcat caagtcaata atgattgggc
     6301 aggagcgttc tatcctatgg ttcctggaca tgaaatcatt ggacgagtca aggaggtagg
     6361 acatcaagtc cgccggttca aagcaggaga tttagtgggc gttggctgta tggttgactc
     6421 ctgccgtcaa tgcacctatt gccatcagaa tgaagaacaa tattgcaaat ctggagcaac
     6481 ttttacctat aatagtcaag acaatcatct gggaggggtg acctacgggg ggtattccaa
     6541 acatattgtc gtcactgaag attttgtatt aaaaattgat gaatcacttg atcctgccgc
     6601 cgccgcacca ttactctgtg ccggaataac cacttattcc cccttgaaaa gggctaaagt
     6661 aggtaaaatg agtaaagtag gaatcgtagg cctgggtggc ttaggacata tggctgttaa
     6721 attagcccgt gccatggggg cttatgtgac tgtatttacc acttctataa acaagataaa
     6781 agcgggtaaa gatttggggg ctcatgatgt tgtcttgtcc accgacagca atgtcatgca
     6841 aacactagcc aatcaatatg attttattct tgatactgtt tctgccaaac atgatcttgg
     6901 ccaatattta aaccttctta aagtggatgg acaactaacc caggttgggc ttccatcaga
     6961 tccacttgaa gttaccatgt ttcctgttat tttaaaaaga ctgagcatta acggctcatt
     7021 gattggcggc attaaagaga cacaagaagt tttggatttt tgcgccaggc atgggatcac
     7081 cgcagacatt gaattaataa aaatgcatga agtaaattct gcctatgaaa gactgttaaa
     7141 aggagatgtg aaatatcgat ttgttatcga tatgtcaacc ctggattaaa tgaaccaggt
     7201 ataaaaggct tccagttggc cttttatacc acggagcaaa tattattcct ggtttttgat
     7261 cttggggaaa actttaattt tggttataaa agaatcctgt accttttcaa tcaaggcatc
     7321 aaattcaaca aattgaatta tttcgccttc attgggcaat tttccgtggc gcgtgataat
     7381 aagtccatat agtgtattca gttcttcttc atctggcgat aaagtaatat cgcaatcaag
     7441 agcccgttct agagaataaa gggagcaatc tccttttgtc gttatagttc cgtccgaatt
     7501 ttttacccaa tcttcatgtg ttttatgaaa ctcgtcttta atccgcccaa tcaggatatg
     7561 gagcaaatta tccagagtta caaatccaag taaactcgat ttgtgtttgt ataccaaagc
     7621 aaaatgaggc ataccagctc tgaatttacg cagcaaatcc atagcaggta atttatgact
     7681 gactttaagt actggtcgca cataaggctt taatgtattg atatttttct ggagcaacaa
     7741 atcggcaaat aaatccttaa catggatgac accaatcatt tccttagtcc cggaatcata
     7801 gacaggataa cgactataac gatattttaa aattttatct ttaatcgttt cgaatggctc
     7861 attgatatct attatgatca tttcatcttc agggcgcatt acctcagtga catttaactc
     7921 agccaaatca agagtatgct cgaggatttc acgttcatcc tcttttaatt ccgattgcag
     7981 atgctgcccg cttaaaatta atttaagttc ttcacttgaa taaaaacctt cgtattcgtt
     8041 ttttatatcc atgccggaca gtttgagaaa aaaattggaa caggtattta ataaccagat
     8101 ggcaggatac attatccagt aaaaccaata caacgggact gcagtccata ttgaaatcaa
     8161 ttcagattgc cgaatggcaa tggacttggg cataagctca ccgattacaa tatgtagaaa
     8221 agaaataaga aaaaatgcaa taaaaaaaga agatatagta acaatatgaa ccgatgaaat
     8281 acccaaagca ataaatagtg gtcccaataa ctccgcaaaa gcaggctctc caatccaacc
     8341 caatcctaac gaagcaagag taatgcctaa ctgacatgct gataaatacg catccagatt
     8401 ttgatggact tgctcaagaa tgcgacctct tagcccgtaa ttttctttga tggcttgaat
     8461 tctcgtagcc ctgagtttaa ccatcccaaa ttcggcggat acaaaaaatg cattgagaag
     8521 caccagcagg aatgctataa aaattaacag aatgttatac attaaaaata gtccggttta
     8581 tgatttaatt tcacagaact tacgaaaaat cccaatctct tcgttaaaaa tatttataat
     8641 tcggtcattt ggtttatata aaatccctca ttaaaaatat tttttgcctt gaccttgtgg
     8701 tttttcgtaa gtcctgtttt ctattcagtc gtaccttcac taaaaacatt cactctctac
     8761 aaatgtgatc gaatccttta tgttaataga atatcgcagt cctggcgagt ttaaaatgac
     8821 tcagccagag ctcaaagata caataattat tatccggttt aatgacaaat tatttaatca
     8881 aagcaaccgt atagaaatga caccaaatac cagtaaatgg cggcccccta ttgacaaata
     8941 gaagcacaat ttgttaattt gactcattaa aagacaaaat taactcaacc tgaaaaaatt
     9001 atgaatcgtc caaacagcaa aacaggccat gacttctgtg caaattctta tgccaatgtt
     9061 ttgctttgca gcgcttccca gtctttagat aatctttcaa tcattattat tatcttgccc
     9121 tctttcccct agagcggtca gctgttgcta attctctgcc tctctaggga ggcttccaaa
     9181 gagttagcac caactgattt caactggagg gtgtcatgaa tcaaaacagt ttcaaaccaa
     9241 agaataattt cgtctcattg gtgatgtggg cattaccttt atcttttttt acttttcaat
     9301 tcttattacg tctttggcct ggattaacca tgcaagacat tatggcgcaa ttttcaattg
     9361 atgccgcaag ctttggcctt atcgctgcat tctattatta tggatacgca ggaatgcaaa
     9421 tccctgtagc cttactgctg gataaatttg gagcccgctt cgttgtattt gcctttgcaa
     9481 tgttgtgtgg tattggaact ttagttttca taggtaccaa taatttttac ttggctctgt
     9541 caggcaggtt tttgataggt gtaggctctg cggctggttt tcttggcgta tccaaagtcg
     9601 tatcagagtg gttcagccat tcacagtact ccagaatgat cggtttctcc tttagcgtag
     9661 gcttgttggg ggcaatctat ggaggaaaac cgcttgccca cctgatacag aattttagtg
     9721 gacaacaagt tggtttggct ttggcggttt tctccatcct cttaggatgt tgcgcacttt
     9781 tgatcttgcg ctctccccca aataatactc actcagtaac aaaaacaatg aatttgtctc
     9841 actgttcctc tttattttca tcgtggatca tctgggctct tgctcttgct aatttattaa
     9901 tggtcggtgc cctggaggga ttcgcggacg tatggggagt accttatctt atcgccaaat
     9961 acgcactgaa caaaactgat gctgcttttc tcatctcgtt tatctttatg gggatgatat
    10021 ttggcggtcc tttactcgct ttcctcagcc aaaaattagg aaactacttt gtaatagccc
    10081 tctgtggtgc tggtttggcc ttcttgttcc taatccttct ttacacctct acccttcact
    10141 ggtggcagct gggcaccctc ttctttgcaa tcggcctgct gtgttgctat caagttattg
    10201 tattcgccgc aggttctact ttggttcctt atgaaggttt aggcgtcacc atcgccttct
    10261 tgaattgcat caatatgtta ggcgggtctt ttttccatac tttgattggt aaattaatga
    10321 atagcagttg gagagggcaa atgagcgcta atggacttaa attatatgaa atcgaatcgt
    10381 atcaatgggc tttgtcactc attccattct gtgccttatg tggcagtttg cttattgtat
    10441 tgattcatat caaaacaaga gcttatgcag ctccaggatt tataaaaaag acttcattga
    10501 caggagtgta gctatggaaa aaattttttg gtctaatccc taccagcata cactcgatac
    10561 caagataatc tcaattaatg gcaacgagat tctattagaa aaaaccatcg cgttttcatt
    10621 ttcaggagga caggaaagcg atagagcatt tatcaatggc cttgaagtac tcagttcacg
    10681 aatagatgga aatcttattt attataccct gcctgatgac cataatcttg cgcccaatga
    10741 tgtaatcacc atgactatag attggcctcg aagacaccgg ctgatgcgtc ttcattttgc
    10801 agcagaactg attctggaac ttgtaaccag aaccagtatg cttgaaaaag taggagctca
    10861 catctcagaa tccaaagctc gaatagattt taaagcgaat tttaatatct cttctatttt
    10921 tagccagctg cttgatgaat ataatgccat cattagacaa gatcttgtta tctataaagg
    10981 ataccatgat gtgacaaatc aacgtcggtt ttggaaaatt gacggttttg ctgaagtacc
    11041 ttgtggggga acacatgttc attccacttc tgaagtgggt tttattcgtc tcaaacgctc
    11101 gcatcctggc aaatctattg aacgcattga aataaccctg atggatgata gtagataatg
    11161 cacccctcgc ataagctgat tgttttgctt tggggcaagg tgagaatgtt atggggcaat
    11221 gagtttatat acattgaatg aacgttgctg aaacgtttgg agcgtgacag taaaataaac
    11281 aaggcgggtt attcaggagg ccaggtcaag cgctaatcga gacaaaaata tagaaaaaga
    11341 atacaaaatc gcctaaatta cagcatttga ctttgtcgcc attaacctgt gagcgttatt
    11401 tatcatggaa ctgccctatc atttctctac tgcctatgtg tcaaaaccag agtttataga
    11461 agcactttct gaggagttag gcgaggtatc caataaaata ggctctctcg ttttttctcc
    11521 tcatcagaag cttgatgttt gttttgcact ggatgtatgg cttgaacctg agatagtaac
    11581 ctttcaatcc atttcagaag cagcaaaaat attgaggcaa gccggaaaat tctggtattt
    11641 gcaccctgtt actgaaattc gccgatcgcg cttgattgag gaacaacttc gcaagtgccc
    11701 atcactaatg tcccattttc cagttacaag tgacattcca cccataggtg tatttagtct
    11761 tcttgataga aacactctac tctttagcgc caaacgacta aaaaaatggc cggatggaca
    11821 atgttatttt attgaagaca aagtcaatcc cccaaaccgg gcttatttaa aattatggga
    11881 agccttgttc ctgttaaata aataccctct ccctggtgaa acagttttag atttgggcgc
    11941 atccccggga ggttggacat atgtgatgca atcactcgga acccaagtca cttcggtcga
    12001 caaagcagaa ctggatacaa aaatcatggc gcttcccaga gtcagttttt taaagcaaag
    12061 cgcctttgcc ctagatcccg aacaactcac agagtcttat gactgggtac tttccgatgt
    12121 tgcctgctat cctgacagag cctacaccct catcaaaaaa tggatagcat caaaaaaagc
    12181 aaaacagttg atttttacca tcaaactaca ggggcaaact gacttaaaca caatcaaacg
    12241 atttcaatcc atacctaatt ccaaagtaat caatctgttt tataacaaac atgaagtcac
    12301 gtttttttat ccagtataaa cataaccagg atttagtgat tatgtctgca aaaatatctt
    12361 aatgacgagc taatatttaa ttttctatga cttaagacct tgaaaaatca aatgagagaa
    12421 ttttttctat gcttggttgt tttaaggcga gcgccaacaa tcgatcaacc cccagggcaa
    12481 ccccgctgca aggaggtaac ccatattcca gggctttcaa aagatatgca tcggcttcca
    12541 ttgccgtcaa tcccttttgc tttctatgtt gctgatccag cataaaacgc tttttttgcg
    12601 cctcgacatc agtcaactca tgaaaaccat tagccaattc aactccctga tagtacactt
    12661 caaagcgctc agccaagccc tcttttactt gtgccaaggc agcctgggag gcagggaagt
    12721 gatacacagc aacgggggtt gattcttctg ccaaaaaagg ctccacgaca tggctcatta
    12781 ataaaaacag atattgatcc ctgtcttgtt cgttaggaga taagacacca tccaaatcat
    12841 gctgtgctaa taccttgttg aattgttcta tggatgtaga aaaagggtca atgccgcaag
    12901 cctcttcaaa agcctgttga taggtttttc tgatcatagg ccgggattta agaataattt
    12961 gtaataataa atccacttca tccatcaaaa catgatgatc aatatgtaat tgataccatt
    13021 caagtaaagt aaattcagga ttatgccagc gccccagttc gtcatcacga aaaactcgcg
    13081 ctaattgaaa aataggcccg ctgccagcag ctaacagccg tttcatatga tattccgggg
    13141 aagtttgtaa atagcatgtt tcacctctga aaatcgcttt gatattgctc aggtaaacat
    13201 ctgtaacccc atgccgcgcc atcacagggg tttccacttc cagatattgg cgttcagtaa
    13261 aaaattggcg gattttacct attagctcgg ctcgcgcacg taataattga attgaagctg
    13321 agggcaacca ggagttatca gacatgatca ataaaatatt cccaactcca ggcgggcagc
    13381 ctctgtcatg cggtcttgag accagggcgg ctcccacacc aactcaacag tgcaatcact
    13441 gactccttca acctgattga ctgcctgctc caccgtaccg ggaaaggttt gcgccacagg
    13501 gcaccccggg gtcgttagcg tcatctgaat atgaacatgg ccttcgtcat taacggcaat
    13561 gtcataaatc aagcccagat cataaatatt gaccggaatt tcgggatcaa aaactcccct
    13621 caacgcggta ataacgcctt cctttattga ttcgttactc tgttttttct taaaaccaaa
    13681 catgagctgc ctctggtcta ttttgcaata tacagacaaa ctctttcacg agctctgcca
    13741 ctattgccat aacattattc tgttttcacc acttctgttt ctttgtttaa agccgcttcc
    13801 aaagtatgcc acgccaaagt ggcgcatttt acacgcgcag ggaatgcttt aactccggct
    13861 agaactgtta atttatccat ggaaaccaaa gaatcttctt cctcttgtgt tagcattcga
    13921 tgaacacgat gaaatagttc atgagcttct ttaattgatt taccctttaa cgcatcggtc
    13981 attaacgatg cagaagcttg tgagatagca cagccacatc caataaaact gacatcacga
    14041 atcaaatcac cctgcaactt gaggtaaaca gtcagtttat ctccgcataa agggttaaac
    14101 cccttcgcct cggtggtcgc atcctccatc gcatggtggt tacgaggatt acgattgtga
    14161 tcgatgatga tttcctggta aagttcccgc aactccatac tcatgcgaat acctctttaa
    14221 ctcgttgtaa ggcttccata caataatcaa tttctttaaa cgtattataa aaggacatgg
    14281 aaatacgaga ggtcgcagca acatcgtaga aatccatcaa aggcatagtg cagtgatgtc
    14341 cgctgcgtat cgctatacct tcactatcca gaatagttcc aatgtcatgg gcatgaattt
    14401 ttccgtggac aaaagagata atgggtactt tgttggcagc cgttccaata atattgtacc
    14461 cttttactgc ttcaatagcc ttggtagcat agtttaacaa ttgtgtttca tattcagcta
    14521 tcgcatccaa atccaatgac caaatataat caattgcagc agccaaacca atcgcccctg
    14581 caatattcgg ggtgcctgct tcaaatttat gggggatagc cgcatattcc gttgcttcaa
    14641 aactcacgga gttgatcatt tcgcccccgc cttgataagg agtcatgcta tttaataatt
    14701 cttcttttcc ccataaaaca cctattccag tgggaccata cattttatgt ccggaaaaag
    14761 catagaaatc gcaatccaaa tcctgaacgt ccacaatcag gtgagccgtt gcctgtgccc
    14821 catccagtaa cactttagcc cctacttcat gggccatctt aatcattgtt ttaacaggat
    14881 taatggttcc taaagaattg gaggcgtaat taatcgcaac cattttagtg ttttcattta
    14941 actttctttc aaactcttca agtattacct cgccatttaa agaaataggg gcaacttgca
    15001 gcttgcaacc cattttcttg cagaccattt gccagggaac tatattggaa tgatgttcca
    15061 tatgcgttat caaaatttct tcatcaggta atatgcgagg ggctacgaga ctctgagcca
    15121 ctaaattaat ggcttcagtg gtaccacgta caaaaataca ttctctcggt gacctggcat
    15181 taataaatcg ttttaccttt gctctggctg cctcatacat ttccgtagcc ctgacactca
    15241 aggcatgaac tccacgatgt acgtttgaat tatccttttc ataaaattga gcaatagcat
    15301 caatcacagc cttgggtttt tgagtagtag ccgcgttatc aaaatacacc aaatcatact
    15361 cattgatttt ctgatgcaat acaggaaaat ctttcctgat tttattgaca tcaaaggatg
    15421 tcattaggga aggattagta ctcatttgcg taccccaact gttgagttaa taaattaccc
    15481 atccaatcag ccaaatcacg atttggaata aggcgcaaat tatcattggc aaacgcctgg
    15541 atcaaataat gagaagcttc atccctgcta ataccgcgtg ttgccaggta aaagattgcc
    15601 tcttcatcca gttgcccaac cgtagcacca tgtgaacata aaacgtcatc agcgaaaatc
    15661 tctaattgcg gttttgtatc aatttcagca tttgcggaga gaagtaaatt tttattttgc
    15721 tgcttggcat cagtatgttg cgcattcttg gctacaataa ccttgccatt aaatacagct
    15781 ctggaatgtc cctttaaaat ccctttgtaa tcttgatcgc ttcgacagtt aggcaccaaa
    15841 tgatgaacca cagtatgatg atctatgtgt tgcccatccc ccggtgcgta aattccattc
    15901 ataaggcatt gagcctgttc ttcctgcatg gacacgctaa tatcactgcg tactaacttc
    15961 ccacccaaac ttaaagagtg gctggaaaat tgactggcgg cagattgcct aaccgataaa
    16021 tgacctaaat ggtaagctga tttactttct ttttgaattt tataatgatt aagttttgcg
    16081 cctgctccaa gaaacacttc agtaatactg ttagtgaaat agcaaatgtt ttcttcgcca
    16141 tgatattctt caatcagagt agcctgagat tccttttcca taaggatcag atgtcttaaa
    16201 tacatagcct ggttcgtttg atcttggata tgctttaaaa caatgggttc ttccaaacaa
    16261 attccctcag gaatataaat aaagactccg cattgaagca tggctgtatt taaataatga
    16321 aacccatgct cctgctccat aatttttcca aggtgaggct taaccaaatc agcataatgt
    16381 attaacgcaa gagataaagg cagaattaac acgccttgag gcagattctt aatccattct
    16441 tgctcttggg aaataaatcc attctgtatt aaaaacagat ttttgactgg tgaatcgtca
    16501 aaggcggttt taagagcagg ctttactgta ggatcaggtg gtacaaaatg ttgctgtaat
    16561 agtgagtcaa cgagagtgta cttccattcc tcatcatatc gggtgggaaa accacggcgc
    16621 ttcaaatcag ttaatgcttt agactgcaac tgcgcaagcc agggtatttg cgatacgcct
    16681 aattgggctt gctgttgata ataatccaaa atatcactca taactgctcc acttcttcaa
    16741 gccagctata acccttcttc tccagttcca gcgccaaaga tttatcccct gacgtaataa
    16801 tacgaccatt tgccaagaca tgaataaaat caggctcaat ataatccagt aagcgctgat
    16861 aatgggtaac taaaataata gaacgctcgg gagagcgcat cgcattaact ccttgagaaa
    16921 taattcgtaa tgcatcgata tccaggccgg agtcggtttc atcaagaata gctagcttgg
    16981 gctccaaagc ggccatttgc aatatttcat tacgtttctt ttccccacct gaaaatccct
    17041 cattaatact tcggtataaa aagctttcat ccatatccag cagctgacat ttttcccgaa
    17101 taaaacttaa aaactctatg gcatcaaggg tgttttttcc ttgtcctttg cgaaccgcat
    17161 tcactgaggc tttaagaaaa ttgatattgg ttacaccagg aatttctacc ggatattgaa
    17221 aagacataaa aataccggcc cgcgctctct cttccgggga cataggcaat aaatcttgtc
    17281 ccaaatagga tatatcacca cgagttactt cataagaagg atgcccagcc aataccttgg
    17341 agagagtact ttttcctgaa ccattaggtc ccataatcgc atgaacctct cctggtttaa
    17401 cctcaagatt aatacctttt aaaatagact gtccattgac agcaacattg agttctttaa
    17461 tgtttaacat attagccgac tgccccttct aagcttatac ctagtaattt agtggcttcc
    17521 accgcaaatt ccataggtaa ttcttttaaa acttgcttac aaaatccatt cacgatcata
    17581 gaaactgcat cttctgtatc gataccgcgt tgctggcaat aaaacaattg ctcttcacta
    17641 atttttgaag tagtggcctc atgctctact tgtgccgttg gatttttgac ttctatataa
    17701 ggaaaagtat gtgctgaaca ttgactaccc attaacatgg agtcgcattg ggtataatta
    17761 cgcgcgttcg tggctgttgg tgcgatgcgt accaatcctc gataagcatt atgcgcatgc
    17821 ccggcactga tccctttcga aataattgtt gatcgggtat ttttccctaa atgaatcatt
    17881 ttcgtacctg tgtcagcttg ctgaaaatta ttggttaagg caacagaata aaactcgcca
    17941 acagaatcat caccttgcaa aatcacactg gggtatttcc acgtaatggc tgaacctgtt
    18001 tcaatttgtg tccaggaaat tttcgatttc ttacctcgac aagctcctcg tttggtcaca
    18061 aaattgtata tccccccttt accttcttta tcaccgggat accagttttg taccgttgaa
    18121 tattttattt gagccccatc taacgcaatc aattcaacga ctgcggcatg caactggttt
    18181 tcatctcgca tcggggcagt acacccttcc aggtaggaca cataactgtc aacatcagca
    18241 ataataagtg ttctctcaaa ttgcccggta gaagcagcat tgatcctgaa ataggtcgat
    18301 agctccatgg gacatcggac accgttaggg acataaacaa atgatccatc actaaacaca
    18361 gcagagttca aagcagcata aaaattatcc ctgtaaggaa caactgaccc cagatatttc
    18421 ttaaccaagt ccggatattg atgtaccgct tccgataatg ggcaaaaaat tactcctttt
    18481 tcagctaatt tcgctttaaa agtagtcgca actgaaacac tgtcaaacac cgcatcaacg
    18541 gcaacacctg ccagcatttc ctgttcgcgt aaaggaatac ctaatttctc ataggttttt
    18601 aacaactccg gatcaacttc atccagactt ttgggagcgt cttttttact tttaggcgcg
    18661 gaataatagg aaacggactg ataatcaatt ggaggatagt gcacactcga ccattcgggg
    18721 tgaggcatag tgagccagtg ccgaaatgct ttcaaccgcc actcaagcaa aaattcaggt
    18781 tcgcctttta ttgcagacaa acgacgaatg acatcctcat ccaatccggg aggaaaagtt
    18841 tcaacttcta tgtcggttat aaaaccatgt tgatattctc tatcgagcag agaatttagt
    18901 tgctcactgc ttttagccac gatttactcc acttgctaat tgccgaactc gctcaacatc
    18961 ccttgttgga agagttggtt ttgccaatgc ctccaaactg acactatcaa gagcagtttc
    19021 tatcgcatgg cttattaatc gccaatttcc ttgaatggta catactccct gtaaaaaaca
    19081 atcattgggt tgcatactgc attctgtgaa acccctgtgc tcttccaaag caaatatgac
    19141 ttgagacacc gaaatttcag ctgctggtct ttgtagtcta tatcctccgg atacacctcg
    19201 taccgaagtt aacaaacccg cgctagtcag gcgttttagt attttactaa cagtaggtac
    19261 cgttaaatgc gtatgtaagg caatatctcg agcattgcat aattcctgcg aatgcttggc
    19321 tagataaacc attacaactg ttccataatc ggccaattta ctgacgcgca gcataacacc
    19381 ccctactcga ataatctagt accaaataag tcttattttc agatagagta aaagaatatc
    19441 ttattaagaa caaggattag acaattgctt actgatccaa tatcacctga tcctctaagt
    19501 gagcttttct ggtacaatca tccaaagcaa aattctatta ccttattata aatatagtac
    19561 taatttggtt ctatattcga taaaagagag attttacctt attgataagg aaataagcaa
    19621 ataaaatctt ctgctctatc ttggcgattt catgcgtttt aatatttttt acaataggtt
    19681 agtcataaag ggaatgggct tgctaatatt ccatctgtct ataatgtgct ctggatttaa
    19741 tatagaatac tcttgataaa cacaaccaaa cccataagaa taagctattc gattatttgc
    19801 tttcaggagc taggatgact cacgatactg aactaagcga actgatgcag gccgtagcta
    19861 aaaaaagcct gcagattatg accgatttca aagaaaaacc catcccgctt tcatcgctgg
    19921 tcagtcaata catcgatctc acggagcatt tccaaaattt gattgccgtc attctaaaaa
    19981 atcctgaaaa agtatggcaa atgcagttga attacctcga agatgcctta agtctggctc
    20041 aagcacaatt taattattgg cttgaaggaa aaccactgcc tattaacgat cagcgtttta
    20101 atggggaaga ttggataaac aatccttttt ttaacttgct aagccaacat tatcttttgg
    20161 caaatgagca tatgaattca ttgctggaaa atatggaata tggtgacgag aatctggcaa
    20221 aaagagtacg tttttttacc cggcaatatc ttgatgcttt atccccagct aactttattc
    20281 atacgaatcc tcagctcatg gcagaaacat tacaaagcca tggcaaaaac ctattacgtg
    20341 gattacataa tttgctgtcc gatgttgaag caggatcctc tagattaatc attaaaatga
    20401 ccgatactga agcatttaaa atcggtgaga atctggctac aacgcctggt aaagtcatat
    20461 ttcgcaattc catgatggag ttaatccaat attgccccag aacaacaaaa gtaaaatcca
    20521 tacctttact tatcgttcct ccctggatta ataaatacta cattttagac ttaagtccgc
    20581 ataattcatt aatacgctgg ttggtcgaac aagggattac tgtttttatt atctcctggg
    20641 tcaatcctga caaaacgtat gccaataaaa gcttctatga ttatttaaat gaaggcccca
    20701 gagaagccat ttcagtaatt caaaaacaat tgcgtgttaa acaagtcaat acgctgggat
    20761 tctgtatagg cgggacactt ctggcttctc ttttagccta caataaggca actaaggatc
    20821 tttccattcg cagtgcaacc tttttagcag caatgattga tttcagtgat cctggagaca
    20881 tcgctgtttt tattgatgag cagcagatta ataaacttga agaagaaatg aaatccaaag
    20941 gttatctggc tggcaaattt atggcgtcca gctttaattc cctaagggca aatgacctaa
    21001 tatggtcatt tttcatcaaa aattacctgc gcggtaaaaa ccctgttcct tttgacatac
    21061 tctattggaa tgctgattca actaatatgc ctgcaaccat gcattctcaa tatttacgtt
    21121 ggatgtattt gcataatgac ttgataaaac ctggaaaaat tcgccttaac cataccccta
    21181 ttgacgtaac aaatatcgac atacccactt tctttctgtc tactcaaaaa gatcatatcg
    21241 ctccctggaa aacaacatat aaaggatttg aattaatgaa ggggccaaaa cgttttgttt
    21301 taggtggatc tggccatatt gcgggaatta tcaatccgcc aacccagcaa aaatatggtt
    21361 atagaaccaa tagcagcatg gatttatctg ctgagcaatg gtttgaaaaa tccaaggaac
    21421 actccggttc atggtggcca gaatggttaa attggcttaa attgcattcc ggaagactca
    21481 ccaattcccc tgatatcaat catttgccat tcgcacctat aatggatgcc ccagggagtt
    21541 atgtactaaa aaaataatat tttgaactca ataaccatac attaaaacat aaaaggagtt
    21601 gtattgtgag aaaaaaaaac aggatacttt tggcaagttt attcttgcta ggcagcctga
    21661 atgcctttgc ctctcaagat ctgacggttg ttaatacgtc cgatgatctg ggatatgtga
    21721 caataggatc ctattcctgt gaattgactt ccagttgtgc tattccccct gataattcga
    21781 ttcgtattaa ctctgatatc gtagaagcag cttgtgcgaa tacccccgtt gattgtgaaa
    21841 ttgtttttgg ggttgacagt ataaaagcaa gagtcgcgac cggaagcttt agcttgaatg
    21901 aggggataca atatattgcc caatctcgtt ggctgaatta ttctgctaag cgtttatcca
    21961 aaaccgtggc tgaaatctat aaaagataat tcgatttatt ccttgctgat gtcctggttg
    22021 aagcacgcag caatagccag gacaatgttt attgaaatga attgattaag ataaatcata
    22081 aacacaggga tctgattttc tccaccagct ctctgttctt gccaaagtca gaatacccca
    22141 tggttgcact tgaaataaca ttcccatccg catggatgca taaccaatca acaaaatgga
    22201 aaaagcggct ttgtctatac cccaccagta aggatgcctc ctgcttctga attttcaatt
    22261 ccggaatctt gcgttcaatg caagctacca attgaggcat actctcaacc ttcaaaggag
    22321 caatatagtg agagtccgat tcattaacca tgctacttac ccagtttggc ttatcatcgg
    22381 gcaattgccc ctcaactaac cccttcggca tttctcgaaa gttgatttga ggaaataaat
    22441 aaagaatgac aacaaaggct aaaataaggg ctaatactat ccatattaat ttcatttata
    22501 ctgtttccat ggtttctttc tgttctaaaa attgcttttc aagcgttata taatcaattt
    22561 ttcctgaagg caggacagga atttggcatg ccggataaat catatgagga accagcaatt
    22621 ctgattgccc tctctcctta acctttttta taaatgatac tttatctgca tactctgctt
    22681 cagtaaataa gaaaatttgc tccccctttc ttggagattt ttgcacaaca gcggcatgta
    22741 ataattcagg ccaaattgaa gctgcaattc cttccaccgc tgtaagagac accatttcac
    22801 cagcaatctt ggcaaaacgt ttggcgcgac ctgctatagt aatgaatcca tccgcattca
    22861 cggtgacgat atctcccgta tcatgccaac catcttcagg ttctataatt actccaggtt
    22921 tatcctcatc aagataacct aacatgatat tgggtccgca taattttaaa cgcccgcctt
    22981 gtacaattcc ctctactggt tctactttga aatccataaa aggtaaaatc attccgacac
    23041 tacccggtac tgatgccaac ggacaattaa gcgatatgac tggcgatgcc tcagtagcac
    23101 catagccttc ataaattctt gccccaaacg cttcgctcca gtatctaatg gtttccggtt
    23161 tcactttctc ggcgccagcg aagatatagc ggacgctgtt gaaatcatgt ttgcccgcgg
    23221 cccttgcata acccgttaaa aaagtatctg tgccaaacaa aatagtagcc cctgtttgat
    23281 aaaccaatcc aggaattacc ttataatgaa gaggggaagg atagaaaaaa caattcaaac
    23341 cattaaccaa aggaagcaca cttcctgctg taaggccaaa acaatggaat ataggtaagg
    23401 aattaaataa aacatctcgt ggagtaaagt cgactctgga agtcatttgc caacaattag
    23461 ccaatatatt ggcatgacta agagcaaccc ctttgggttt tccctcagaa ccagaggtaa
    23521 acaggattat gcctgttgta tcaggggata ttttgtcacc aagaaaacgg tagcttagag
    23581 taggaaagaa gcctttgaat atcccggaaa gcttgttacc taaatgaatg gttgatttaa
    23641 aatcttctaa ataatgaatt cttatacccg cctgttgtaa ctcttctacc aagggttcta
    23701 atctggcggt ttctatgaat tgtcttgcgg tataaattga tttaattctt gctgtattac
    23761 aagctgaata taagttataa aaacccatgc taaaatttaa catggcaggt attcgtctat
    23821 aagcttgtaa agcaaaaaaa gtgaccatgc cagctatagt agtcggcatc attactccaa
    23881 ccgtttcgcc tatagccgtt tgctttttaa tttggcgccc cagaataaaa caacgagcaa
    23941 ggaactgtct gtaagttaag ggaatacgat tattgtcttc tattttagtt ttgtttttag
    24001 ctcctatact cacgccttca attaaagcag aaaataaaac atatggctta aaactattgg
    24061 cgaatgacaa atcacttatt agttgaaata aacgtaaact gataccttct ctgtcgacac
    24121 ctgtattttc atcttgaatc aatttttgag caggtaatat atgaagagta attttcggaa
    24181 aaatccgtat tcgatgttta tttttgctat tagaaaaaat gctgtgctcc gccccttcaa
    24241 tacgaacagg gattacctcg gcgccaacct tttggagtat caatccaggt ccttcaaaga
    24301 cctttaagct gtcttcctgc tgtcttaata gcccttgtgg aaatagaaga caacgcttgc
    24361 cagcccgtat tgcccttatt aaaactcttg ttgcctgagc gctgcctgga tcaactacta
    24421 tgacatcagc aaaaaataat aacgttttta cccatatttt tttaaacata tcaggatgta
    24481 aagctaaagt taatcgttcg ggtaaaaata cagaaaggag taacacatca attatagaag
    24541 ccctgttggc aataatgaca gaatggctgt tggcctgata ttgtccttta acctctatgc
    24601 gaaaccattt ctccagaaga atttttatcc atgccctagc ccgctgtttt tgtttttcct
    24661 tccaggtcaa attttcctca tttgtttttg tcatcatgtc gcatcagtta tctctatatt
    24721 gacttttatt ctgcaagatt caaaacgatt tgaatagata ggtttatgaa tcatgttgat
    24781 ttcatttaaa aaatttaagc gaaagcaaca tctgttttta tgataattta cccggaatat
    24841 tgagagaagg cttgcgagtc cttaattcct gtaataaagt gaggggcatc tttactttaa
    24901 cacaaaaaac cattaagaca ttctgtttaa ttttagccat attagtttta attattttcg
    24961 cagtggtctt tcataatcat tcgttagaga tcattgatta tatagacgat ctgggttggc
    25021 ttgcccctgt tcttttcttg atcacctact gccttgcgac tttattgctt ttacccacaa
    25081 tggtattaac tctggctggg ggagctgtgt ttgggcctgt atttggcacc ttgcttaatt
    25141 tattgggagc cactagcggc gctgcgtttg catttctgat aacaagacac ctggtttacg
    25201 attggttctc aaccaaaaag ggtgaaaaac taaacaaatt gattgccggg gtagatgaga
    25261 aaggttgggt ttttgtggct ttcttgcgat tgtttccaat agtccctttt aacctggtca
    25321 actatggctt aggcgtaacg ggcatatcat ttcgtcttta tttgctaact acttttattt
    25381 ttctaattcc tgcagaaatt atttacacct attttggata cgtcggcatg ga
//
LOCUS       DACTJE010000027        25339 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.027, whole genome shotgun sequence.
ACCESSION   DACTJE010000027 DACTJE010000000
VERSION     DACTJE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25339
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.027"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..489
                     /locus_tag="I9760_000865"
     CDS             <1..489
                     /locus_tag="I9760_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5042645.1"
                     /translation="ALRKQIVNMIENIQVNEFHLSITPEINASILKAEEEYCYYSSCN
                     FWYAVDEQGGIIGSIGLKKLNHTEGEVKKFFIAPKYRGLGLAQKLMLTLVRNAMKHGF
                     KKLFLGTVAQLNAARRFYEKNGFVLVNKNSLPKEFELCPLDTHFYYCETDKLDKSLRE
                     LI"
     gene            547..1110
                     /locus_tag="I9760_000866"
     CDS             547..1110
                     /locus_tag="I9760_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT5042646.1"
                     /translation="MVADLMLCTNFFIFTGGPGSGKTAVLNELNTWGYLTVSEVARSI
                     IQKQQADGGDAIHTGNRKAFRDLMLEQSIADYCKMQSEEKIVFFDRGIPDLYGYSKAF
                     CGEASKEVNEAVGRFRYNKTVFIFPPWPEIYENDDERQQDYQEALQTYYAVKEGYFDC
                     GYVLLEVPKCPIKDRANFIFQMISKNG"
     gene            1352..2659
                     /locus_tag="I9760_000867"
     CDS             1352..2659
                     /locus_tag="I9760_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042647.1"
                     /translation="MAAGFIGLLKQRKFLPLFLTQFFGAFNDNAYKLAMLTMISYHLS
                     HTQEQSEYYQAIAGALFILPFFIFSATSGQLADKYDKALLTRLVKVLEVLLMIIGGIA
                     LYSGSIFLMMCTLTGMGVHSSFFGPIKYAILPDHLPKKNLLAATGLIEASTFIAILLG
                     TTLGTLTVGGKEATPYIAIIMTLSAAFSGLVSSLFIPPAPSALHELKVDFNLWRATVK
                     MLKEAKKEYGILISILTISWFWLIGAIMLTKLPDYTHYVLGASAHVFALFLALFSIGI
                     ALGSITINWLLKGQVNLSAVPMAMLAFTFFAFDLYWSSPAGHDLKTPLFNLFNFFTSF
                     THWRIAFDLFMLAFCAGLFVVPLYTYLQIISPVENRARTIAANNIFNALFMVMGTLLV
                     MLLLFFSFSIPQVFLILCLLNLVAAMFLFLGLRTLRRSSEEIA"
     gene            complement(2804..3739)
                     /gene="hemF"
                     /locus_tag="I9760_000868"
     CDS             complement(2804..3739)
                     /gene="hemF"
                     /locus_tag="I9760_000868"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAT5042648.1"
                     /translation="MPISKTLPYNAIEQIKSYLLQLQNTICVSLESIDGKTRFHEDSW
                     QRAAGGGGKTRIMANGNVFEKAGVNFSHVSGEQLPASASAHREELAGRHFSALGVSLV
                     IHPQNPYVPTTHANVRFFVAEKEDSEPVWWFGGGFDLTPYYGFVEDCEHWHQTALNAC
                     LPFGETIYPKFKRWCDDYFFIKHRNEARGIGGLFFDDYNEISFDHSFELMRSIGDHFI
                     LAYEPIVARRKDIPFGNREKAFQNYRRGRYAEFNLVYDRGTLFGLQSGGRTESILMSL
                     PPIVHWEYNWHPEKGSDEEKLYTDFLPAKDWLKKE"
     gene            3954..4346
                     /gene="flgB"
                     /locus_tag="I9760_000869"
     CDS             3954..4346
                     /gene="flgB"
                     /locus_tag="I9760_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAT5042649.1"
                     /translation="MAINLDSYFGIHAKALIARDQRASVLANNIANVNTPNFKARDVD
                     FNEVLTATMAGNSQQLRVTSDQHINTNADFMTHLKYRMTHHASLDGNTVDKDMETTEF
                     ARNALNYQASLSFLNGKIKSMLTALKGE"
     gene            4349..4771
                     /gene="flgC"
                     /locus_tag="I9760_000870"
     CDS             4349..4771
                     /gene="flgC"
                     /locus_tag="I9760_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAT5042650.1"
                     /translation="MSLNAIFDIAASAMTAETVRLTTSAGNMSNANVEAGSPDEVYQA
                     KYPVFKSVQENANQWMGEQIKAGVKLDGIYESDAEPHRRYDPNSPIADKDGFVYTSNI
                     NYVEEMANIISASRSYQMNIELLNTTKQLMQRTLQLGE"
     gene            4783..5460
                     /gene="flgD"
                     /locus_tag="I9760_000871"
     CDS             4783..5460
                     /gene="flgD"
                     /locus_tag="I9760_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HAT5042651.1"
                     /translation="MTTNSVNGSNSAGGLGLNQIDSGKRQNLGQQDFLRLMVAQVQNQ
                     DPLQPQANGEFLSQLAQFSTNDGITKMQESLQQLASSLQSNQALQASALVGRKVLVNS
                     NTLSLGTEGDVKAAIDMAPGVSNLRAAIYTESGELIKTIPLGQPEPGFFQFSWDGTDQ
                     SNQRLASGRYTIKVTGTYGGQEVALKTMTSANVDSVSLGQNGEDLKLNVAGVGSVYLS
                     QVRQISV"
     gene            5574..6887
                     /locus_tag="I9760_000872"
     CDS             5574..6887
                     /locus_tag="I9760_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HAT5042652.1"
                     /translation="MVFNTALSGIQASTRDLEVIGNNIANSATIGFKGSRAEFADIYS
                     SSAYGSGSNAVGGGVKLSRVHQSFATGTLSTSNNTLDLAVNGSGFFILSDQGAKIYTR
                     AGQFKLNNENYIVNANNQRLTGLLTDSNGAITGASGDLRINTANITPRASSNVSVGVN
                     LNSQSKAPNVDWVGGASPATDTYNNVTSSTIYDSLGNSHVLSMYFIHADSTALAGSPN
                     AASPAGTENQWYVAFQIDNQNVPPNVGAQNTNNLFRANFNSDGSFAGVFDTSNAALPN
                     NLIPLSFNLNNGSSPLNLNIDLSDSTQFGSPFAVQSAYNNGYTTGSLAGLDIDDSGMI
                     YGRYTNGQSLAMGQIQLANFADPESLQNIGNTSWAETTASGQPLISAPGSGGLGLINS
                     GRLEESNVDLTGELVKLINAQRNFQANAQTIRTGDAITQTIINIR"
     gene            6898..7644
                     /gene="flgF"
                     /locus_tag="I9760_000873"
     CDS             6898..7644
                     /gene="flgF"
                     /locus_tag="I9760_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HAT5042653.1"
                     /translation="MEPILYNSISGSRSDFKRQEIIANNLANINTPGFKADLYQAQTM
                     YMNNANGNNQFSAHSFITQNANGIDTSPGEIITTGRDLDVAIDGDGWMAVQDSQGREA
                     YTRGGSLRLNPNGQLITASGKVVLGDGGPISIPPAQSIEIGSDGTISIVPLEGDVKSL
                     AILDRIKLVTLDRNNIYKNDEGLIQLKSGAATANSNIKLQSGALEGSNVKAIDQMVAM
                     ISAGREFDAQMKLLSTVDDNGQKLAQLLQE"
     gene            7791..8576
                     /gene="flgG"
                     /locus_tag="I9760_000874"
     CDS             7791..8576
                     /gene="flgG"
                     /locus_tag="I9760_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAT5042654.1"
                     /translation="MEPALWVSKSGLEAQDKNIATIANNLANVNTTAFKKGRALFEDL
                     IYQNLRQAGAQSSQNSQIPTGINMGTGVHLVATEKIFSQGSLQNTQNALDVAIEGRGF
                     FTVLMPDGTQSYTRDGSFKTDSTGQIVNSNGYPLQPNITIPPQTTKITIGTDGTVSAL
                     VAGNNAPSVIGNIQLTDFINPAGLQPIGQNLYTETVASGTPITDNPGNSGLGTLLQGS
                     LEASNVNVVEELVNMIQAQRSYEITAKSIQTVDSMLQYLTQTL"
     gene            8589..9281
                     /gene="flgH"
                     /locus_tag="I9760_000875"
     CDS             8589..9281
                     /gene="flgH"
                     /locus_tag="I9760_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAT5042655.1"
                     /translation="MNRLNIAVSCLATALLFGCEALHPPAPGDNPDYAPTYPVTPDPK
                     ELRKVSGAIYSSETALPLFETPRARHPGDILTVYLIEKTDAQKNATTTQRKNDTTKIT
                     NKLFLGRPISLGSGYSMDFDLDNQRQFNGEGRSIQNNKLAGSISVTVAKVLANGNMVV
                     QGEKWVRINQGNEFVRLSGIVRPQDIKADNTITSDRIANARISYGGTGQINNTNAQGW
                     LSRILWGPLFPT"
     gene            9320..10423
                     /locus_tag="I9760_000876"
     CDS             9320..10423
                     /locus_tag="I9760_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAT5042656.1"
                     /translation="MRRMLVIRWILAIHLIATQVFAERIKDIATLAGVRVNQLVGYGL
                     VVGLSGTGDKTGTKFTEDSFANMLTQLGINIPPGVRLNSKNIAAVMVTANLSSFMKKG
                     QTMDVNISSIGDSKSLLGGTLLLTPLKGADGRVYAMSQGNVVVSGISASGSDGSSVTV
                     NVPSGGRIPNGATIEADIPNPFYYSNSLTYNLHNPDFTTAKRMSDAINELMGPGTAKA
                     IDAGSVVVTAPKKLSQRVDYVSVLENIEFKPGEAMAKIIINARTGTVVISSNVIVKSA
                     AVSHGNLVVSITETPVISQPNAFASGRTVSTQQSQVNIQQKNNRAFILPKGTTLKDIV
                     RGINAVGATPADVISILEALQQAGALSATLIVI"
     gene            10435..11310
                     /locus_tag="I9760_000877"
     CDS             10435..11310
                     /locus_tag="I9760_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAT5042657.1"
                     /translation="MTIQSIATSDFQGLNELKIQAKNNAKEALPEVAKQFEGIFLQSM
                     LKSMRMGQHFLDESSPFSGKNEATFQEMLDAQYASTIAESKGIGLAALLAKQLENSVG
                     DKANKPVNSSTEVNNTKVTNSEESLSVVDDFVKSVWPTAKQAASLIGLDPKLLVAQAA
                     LETGWGRFVTRDADGSSSNNLFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYP
                     SIEHSFHDYVSLIKGSERYQMALANAENPEIFVSELNKAGYATDPNYSNKILSIYHGD
                     ELNQAIQRCESFEQS"
     gene            11360..13309
                     /gene="flgK"
                     /locus_tag="I9760_000878"
     CDS             11360..13309
                     /gene="flgK"
                     /locus_tag="I9760_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HAT5042658.1"
                     /translation="MSILNIAYSGLNAFQRALDVTGNNIANFKTRGYSRQSIQFTPIT
                     SNRYAGSYIGAGVSVASIYRNVDQFANAQVRSTLSYRTQYDAFYNQAIQIDKLLSQDG
                     SSISVPLQTFFDSIGQLNSTPDNIATRGVVLKQSQLLAQQFNSLQTKLEEYERNSTLQ
                     LTESVKIINRITKELAEVNGKLLGNNNIPELLDHRDELLKQLSGYTDLSIFDQGDGTI
                     SVGIASGDMLVAGTQQRDLLVGTGKDSIFGTRIFLSSGGNNQVDITDRLTTGMLGGLI
                     DYEKNVLGQASQLIGQMAIGLAQTFNTQHKLGMDMNSQIGKDFFTDFNSPGQMLKRST
                     ASADNSGTAVLSVNISDISQVKLSDYDLIISDTGANELRLIRKSDGTSTTLTWSSSPP
                     APPAGQVVIDGMTITVNDLSQLANNDHYTLTPTRGAARDFALEIKDAYEIALASPVKT
                     TASLNNTGQGQIILGPVLNTASVNKQFRIDFISDTQYNLVNVTDSTTAGPFAFIPNTN
                     NVIQIPDGITPSYSVVLSGIPKSGDQFTTDYNTGGFGDNRNGLILGNIQQNKIFSNGS
                     ETLFDRYGNLLAEVGGRTNQAKTSFESADILHKQALDFQDSKSGVNLDEEGANLLVFQ
                     QAYQAAGKLMEISNQIMNLLFDIMR"
     gene            13313..14548
                     /gene="flgL"
                     /locus_tag="I9760_000879"
     CDS             13313..14548
                     /gene="flgL"
                     /locus_tag="I9760_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HAT5042659.1"
                     /translation="MRISTNQIYQRGLDNLLTQQERLARLQDQQASGIRVRTPSDDPV
                     ASAQIELIKQRINNIELFQNNRQAADSALRLEEGILSNAVNSLHRLREIQIQAGNPSL
                     SEEDRKTLAVEAQALLNQLLDYANTKDSNGSYMFSGSKSLTQPVSLNLSGQYVYNGDS
                     TQRFQAVTTSLLVAVNDTGDNVFMRIPSGNGRFAIRETLTPNTGTASVSSGSVTNEAA
                     FVPDNYTMTFALNSQGNLVVMVSGTLSGNVIPPSGLPDDAPLYQEGSAIGFNGMEMVV
                     SGLPKAGDSFSISPAKNESIFSTVQRMINNLNKPYTSSVEKAATQTENNQLLAQIDSA
                     LGHILSVQSDLGARLNQLETAEKANNDYLDISAATLKKLREIDPVAVATELNLQLVNL
                     QAAQLSFTRIQGLSLFNFL"
     gene            14743..16539
                     /gene="vpdB"
                     /locus_tag="I9760_000880"
     CDS             14743..16539
                     /gene="vpdB"
                     /locus_tag="I9760_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VpdB"
                     /protein_id="HAT5042660.1"
                     /translation="MKTVNSQNGTSAEPSVNLGKLSTAKNLVFRGGGSKGIAYVGALQ
                     SLSEQGVLENIENVAGSSAGAMTAAIVACGGSADLMEYFCSANSLEDFVDNPLTRKLA
                     KLFMDKKGFLAGILIELFKSNKMLPSDSSAAYTSLSHEQTDKQLDAKSSLAEAKLVTK
                     PSKLTFPVASKGTKLVEAMEHVMRMAIFTHFPESLDNELKNVREKLNAIEDKGSAEYI
                     NLAKRQQIVEDTQKIINGLHDPNFDLTFGHLHTLHQYDPQRFKELHITGTDENGELRI
                     FSHEKDADMPVSIAVRISASLPGVFDPVIYEGRKYIDGGAANNLPVNIFFNKEEAYTP
                     GVVTTIGVSCENQLDITEKEFKNPSFMERIKEYLGDFVKKILCKLDVEGAVKEQKREM
                     LKYIDNGNLFVMVAPPISVTTAQMHINDKEKEGVIEVGKAFCDMQLKSMIDADYKPQP
                     FDEKAAKILTNSSQQQEQLKEKTMLFKSRLQSFKQGEGVKPWSQHVENAIDRLMSLKG
                     EITKAQVDLGRTWFDIKSENADPAVRLKKFNDAFLASPLAKPSSNQQEINFSKEIRKE
                     IDLLKGLPGLNNTSSHCTEEFNEQSTPSLYRA"
     gene            complement(16663..16738)
                     /locus_tag="I9760_000881"
     tRNA            complement(16663..16738)
                     /locus_tag="I9760_000881"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16703..16705),aa:Arg,seq:cct)
     gene            16932..17993
                     /locus_tag="I9760_000882"
     CDS             16932..17993
                     /locus_tag="I9760_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 domain-containing protein"
                     /protein_id="HAT5042661.1"
                     /translation="MNELSLQDLPEYQSFLVEAMQQITQSRIKAAYSVNRELIMLYWW
                     LGEHIVTQQNTYGWGKSFVERFSLDLKKHYPDAKFGFSPQNLWYMRQFYLEYKDKPNL
                     QQLVGEIPWSQNLLILSKIKDDNEKLYYLTAAKEQSWSRETLREQINSQAYQRHCLSE
                     KNHNFKNTLPRDLAAQAQQSMKDVYMFDMLGITEPVVEAEIERRMVEKIKDVILELGY
                     GFSFIGNQYRIVTPDSEYFIDLLFYHRKLQALVALELKRSKFKPEYAGKMNFYLNLLD
                     DFVKEPHENPSIGIILCGDHSKFDVEYALRGIDKPVGVAGYQLTRDIPKQLRDALPDA
                     DQLEEKIMFELGLNNESQN"
     gene            17977..19194
                     /locus_tag="I9760_000883"
     CDS             17977..19194
                     /locus_tag="I9760_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT5042662.1"
                     /translation="MKAKINNTLLKKLVPQDKEYEVHDTDLKGFILRVFPSGTMRYVC
                     QYKRGGKINIGTVGVMTPAQAREKAVEILNDFNKGIDPKAKRGSNKLKSLGEFFENEY
                     TPWVLTHHKRGDKTLATIRRCFDKLFSKPLEEITPSIIEQWRIKRLNDGISNATLNRD
                     IGTLKSLFTKAVEWGFIKENYLKNLKLSKIDRAPKVRYLSIDEERNLRRALFEREGKL
                     KQDRKRGNEWRQARGYDLLPEYEENELCDYLTPMVLISINTGLRQGELFHLTWEMIDL
                     QERSIIIAGQITKNSSSRYIPLNNEVYGIIKQLYEKSNSKKGLVFLSKDNKPFNNIKR
                     SWTTILKKAQITEFRWHDLRHHFASKLVMAGIDLNTVRELLGHSDIKMTLRYAHLAPE
                     HKIKAVNKINWTD"
     gene            19592..20260
                     /locus_tag="I9760_000884"
     CDS             19592..20260
                     /locus_tag="I9760_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAT5042663.1"
                     /translation="MCGRFAYIASYDKLKYQFHLANAIEVPPRFNISPGADVLCLVET
                     DGHKIQCVLLRWGFIPSWATDRKKLGNLINARAETVFEKPTFRQSIKSKRCLIPMSGF
                     YEWRQEDGVKQPYFFQKKNHDLLAVAAIRDTWQQSDEVIHSCCLITTDANAFMQPVHN
                     RMPVILGEEAQAIWLNNTQYDKAQLMALMKPYPYEDLEGYRVTTLVNKANFDHPLAME
                     PLSE"
     gene            20396..20902
                     /gene="umuD"
                     /locus_tag="I9760_000885"
     CDS             20396..20902
                     /gene="umuD"
                     /locus_tag="I9760_000885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAT5042664.1"
                     /translation="MSPRGGKRVGAGRPRGEPTKAVRISLSQLAELERLKSHASYQLP
                     VFASKIQAGFPSPADDYIEGYLDLNTKFIKHPSSTFVLQATGESMVEAGIFSGDWLLV
                     DRSIEPSDGRIVIAAVNGELTVKRLSKKGGRVQLLPANPKFQPIDITEDSEMVIWGVV
                     TLVLHELA"
     gene            20889..22166
                     /locus_tag="I9760_000886"
     CDS             20889..22166
                     /locus_tag="I9760_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAT5042665.1"
                     /translation="MSLPEMFALIDCNNFYASCERLFRPDLKDVPIVVLSNNDGCCIA
                     RSNEAKALGIAMGEPYFKIKHLCKQHGVKAFSSNYTLYGNMSHRVMCTIEEAWPHIEV
                     YSIDEAFLDLRSLPVDSYDSFCEQLQKKILKHTGIPTSIGIGPTKTLAKAANHLCKKV
                     YKIPVFNITSNRGRLLQQISVGDIWGVGRQWANKLISRGIHTAYDLAMTNPHLLKKCF
                     NAVLMRTAMELQGIACGGLEVIEPKQSIMSSKSFGQMQTQIASIEESISSHCARAVEK
                     MRRQQLVATRLVVFVHTNRFREDLAQHFQSIEFKLINPTDDLRLITKIAKRCLQRIFK
                     PGYYYKKAGVCLEDLIPKKPRQLDMFHQPSDEHLKHTEQLMGVFDQINQKYGRSTIRL
                     AAEGYSKPWEMRAELKSPAYTTRWSQVPKVHLY"
     gene            complement(22780..23580)
                     /locus_tag="I9760_000887"
     CDS             complement(22780..23580)
                     /locus_tag="I9760_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042666.1"
                     /translation="MKKIITSAICLYSLSLPVYSESTPDGQPILYCPNSVKCDFKLYG
                     TERCRVMDNPSEVWGGYTFSGPKLLEGIYKFKRVEVSAEKRPVCKYYLNDKQPAPLDI
                     SLNDPNSNYPTTTTRYINKFNPFLAEGTSWTGTNFGMSCSSNDPKHCPLTEEPEITVT
                     SNGSKSFDMVFYFASGNDSEWEPKYKSSSILSYNELYDLCGSTSECIIDIGESSACRD
                     GFDCLQLGTVSLDLSNPNVVKINKTTSSIPYLSIMQKKPFNTLYSELK"
     gene            complement(24118..25317)
                     /locus_tag="I9760_000888"
     CDS             complement(24118..25317)
                     /locus_tag="I9760_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAT5042667.1"
                     /translation="MKIYSFDTLANADLIIDAVYEGGSSGNASDDPISKIIKGIGNMG
                     GFRSAGQGIFKKLIVLYTNMEDGDWPDSIDTSKGQFIYYGDNKHPGHDIHDTPRQGNA
                     TLKMLFDSTHNEKDARRIVPPIFIFVKYPTASSSRSVQFKGVAVPGYPGLSATDDLIA
                     VWKTTNGQRFQNYRAIFTILNIPMVSRKWINSLFDPFGQDNSLNPFYQWKISGKADVL
                     IAPSTKTIRTQIEQMPRTKLEREILQAVFDYFCEAPIKFEACAAKIFQLYDENVLIDE
                     ITRSAVDGGKDAIGRYVLGIKEDPVYAEFFLEAKCYQPGLNGQNINSVGVKEVSRLIS
                     RIKNRQFGVLVTTSFIAKQAYGEVREDGHPIVFLSGGDISRILIKKGINSTDAVLAWL
                     NSEFSKS"
BASE COUNT         8061 a         4457 c         5181 g         7640 t
ORIGIN      
        1 gctttgcgta aacaaattgt taatatgatt gaaaatattc aagtgaatga gtttcatctt
       61 tcgattactc ctgaaatcaa tgcatcaatt ttaaaggccg aagaggagta ttgctattat
      121 agcagttgta atttttggta tgcggttgac gaacaagggg ggattatcgg ttctatcggt
      181 ttaaagaagt taaatcatac tgaaggggaa gtaaaaaaat tttttattgc gcctaagtat
      241 cgagggttag gattagctca aaaattaatg ctgactttag ttagaaacgc catgaaacat
      301 ggatttaaaa agctgttttt ggggacagta gcccagttaa acgcagcaag acgtttttat
      361 gaaaagaatg gatttgttct tgttaataag aattctttac ctaaagaatt tgagctgtgc
      421 cctttggata ctcattttta ttattgcgaa acagataagc tggataaaag cttaagagaa
      481 ttgatatgag tacactaaaa gctttgtaat ttaatttatg agcaattttt aaaggtatga
      541 caggttatgg tagcggattt aatgctatgc actaattttt ttatatttac gggaggacct
      601 ggctctggca aaactgccgt attgaatgag ttaaatactt ggggctattt aacagtttct
      661 gaagtagcac gttctattat tcaaaaacaa caggctgatg gtggtgatgc gatacatact
      721 ggaaatagaa aagcgtttcg agacttgatg cttgaacaat cgattgcaga ttattgtaag
      781 atgcagtcag aggaaaaaat tgtatttttc gatcggggaa tcccggattt gtatggttac
      841 tccaaagcat tttgtggtga ggcgagtaaa gaagtgaatg aggctgttgg acgatttcgt
      901 tataataaaa cagtctttat tttccctccc tggcctgaaa tttatgaaaa tgacgatgag
      961 cgccaacagg attatcaaga agcgttgcaa acttattatg ctgtaaaaga ggggtatttt
     1021 gattgtgggt atgtgttatt agaggttccc aaatgcccta ttaaagaccg cgctaatttt
     1081 atttttcaaa tgataagtaa aaatggatga gccggttggg gtgttggcta gtgtaaatct
     1141 cctgtatgtt cttttgtcat tccaattcct gaaataaatt cgtccaaaat taatatcgcg
     1201 aaataacaat cacaatgtgc tgcatttgta aattaaatag tgggagtggc atcctataag
     1261 ctttttatct ggtatttatc atttactttc attcaataca taaatatcct tgttaactta
     1321 actttagttt taaagcacaa atggagctct aatggcagca ggttttattg gtcttctcaa
     1381 acaaagaaaa tttttgcccc ttttcctgac ccaatttttt ggtgctttta acgataatgc
     1441 ttataagcta gcgatgttga ctatgattag ctatcattta agccatacac aagaacagtc
     1501 ggaatattat caggctattg caggcgcttt attcattcta cctttcttta tcttttccgc
     1561 tacttccgga caattggcag ataaatatga caaggctttg ttgactcgtc tggttaaggt
     1621 gcttgaagtg ttattaatga ttattggagg tattgcatta tacagtggga gcattttctt
     1681 gatgatgtgt actttgactg ggatgggggt acattcttct ttctttggac ctatcaaata
     1741 tgcgatttta ccagaccatt tgcctaagaa aaatttatta gcagcgacag gcctaattga
     1801 agccagcact tttattgcta ttttattagg cactacattg ggtacgttaa ccgttggagg
     1861 gaaggaggct accccttata tcgcgattat tatgacctta agtgccgctt tttctggttt
     1921 ggtctccagc ctgtttatac ctcctgcacc atcagcactt catgaattaa aagtggattt
     1981 taatttatgg cgggccacag tgaagatgct aaaggaggcg aaaaaagaat atggaattct
     2041 tatatccata ttaactattt cctggttttg gttaattggc gctatcatgc tgaccaaatt
     2101 acctgattat acccattatg ttttgggggc tagcgctcat gtttttgctc tatttcttgc
     2161 cttattttca ataggcattg ctctgggatc aattactata aattggttat tgaaaggaca
     2221 agttaatctt tctgctgtcc caatggccat gctcgctttt actttttttg cttttgattt
     2281 gtattggtca tctcctgcgg gtcatgattt aaagaccccc ttatttaact tatttaattt
     2341 ttttacttca tttacccatt ggcgaattgc ttttgattta tttatgttag cattttgtgc
     2401 aggactattt gtagttccct tgtatactta cttgcaaatt ataagtccag ttgagaacag
     2461 ggctagaacg attgcggcga acaatatttt caatgccttg tttatggtga tgggaacttt
     2521 gttagtcatg ctgttattgt tttttagctt ttcaattcct caggtttttt tgatactttg
     2581 tcttttaaat ttggttgctg cgatgttctt gtttcttggg ttaagaacct taagaagaag
     2641 ctcagaggag atagcttaac aacagatctc ttgcataaac tgtctcttcc attttattgc
     2701 gcgttgtaaa cgtttcgcgg tgcttattta ctgcatgtat actggctcct cgaaattttc
     2761 aggtaagggg agaaggatat catttatttt tgagtttggt gttttactcc tttttcaacc
     2821 aatcttttgc aggtaaaaaa tccgtgtata atttttcttc atcactgcct ttttcaggat
     2881 gccaattgta ttcccaatga acaatgggag gcagggacat caatatggac tcagtccttc
     2941 cgccagattg cagaccaaat aaagtgcctc gatcatagac taaattgaac tccgcataac
     3001 ggcctctgcg gtaattctga aatgcttttt ccctgttgcc aaaagggatg tctttacggc
     3061 gagccactat gggttcataa gccagaataa aatgatcgcc tatgctacgc atgagttcaa
     3121 agctgtgatc aaagctgatt tcattgtaat catcaaaaaa taagccgcca atcccacgtg
     3181 cttcatttct gtgcttaata aaaaaataat catcgcacca acgcttgaat tttgggtaaa
     3241 tcgtttctcc aaaaggtaaa caggcattta atgccgtttg atgccaatgc tcacagtctt
     3301 caacaaatcc ataataaggg gttaaatcaa atcctccacc aaaccaccag actggttctg
     3361 aatcctcctt ttcagcgaca aaaaatcgaa cattggcatg tgttgttggc acataggggt
     3421 tttgaggatg aatgaccaga gataccccta atgcgctgaa atgtcttcct gccaattctt
     3481 ctctatgtgc actggctgat gctggtaatt gttcccctga aacatgtgaa aaattgacgc
     3541 cggctttttc aaagacattc ccattcgcca ttattcttgt ttttcctcca cctccagcag
     3601 ctcgctgcca ggaatcctca tggaatctgg ttttgccatc aattgactcc agtgagacac
     3661 aaattgtgtt ttggagttgg agcagataag atttaatttg ctctattgca ttataaggaa
     3721 gtgtctttga gattggcatg atgagactct gcatagggaa ttagcgcttg gatgcttcat
     3781 tttctcattt tttggatatt ggcacaatgt ttgctttgtt taaataggtt tttttatcat
     3841 ttttatgttt cagtgaggtt tgaagacaag tatgaagcca tatagtgcat tggaaaattg
     3901 gcataatgtc aaatgatggt taagacttaa ataacaataa gacaggagta aatatggcca
     3961 ttaatcttga ttcttatttt gggattcatg ccaaggcgtt gattgcaaga gatcagaggg
     4021 catcagttct tgctaataac atagccaatg ttaatacacc gaattttaaa gcccgggatg
     4081 ttgattttaa tgaagtactg acagcaacta tggccggtaa cagccagcag ttaagggtaa
     4141 cttcggacca gcatattaat accaatgccg attttatgac tcatttgaaa taccgaatga
     4201 cacatcatgc ttctttagat ggtaataccg ttgataaaga tatggaaact accgagtttg
     4261 ctcgaaatgc attgaattat caggccagct tgagtttctt aaatggcaaa atcaagtcca
     4321 tgctgaccgc attgaaaggg gaataatcat gtcattaaat gcaatttttg acattgcggc
     4381 ttctgcaatg accgcagaaa cagtgcgttt gactaccagt gctggcaata tgagtaatgc
     4441 gaatgttgaa gcaggtagtc cagatgaggt ctatcaagcc aaatatcctg ttttcaagtc
     4501 agtccaggag aatgcaaatc aatggatggg ggagcaaatt aaggctggcg tgaaactgga
     4561 tggtatttat gagagtgatg cagaaccgca cagaaggtac gatccaaata gtccaatagc
     4621 tgataaagac gggtttgtct atacatcaaa tatcaattac gttgaagaaa tggcaaatat
     4681 catttcagct tctcgttctt atcaaatgaa tattgaatta cttaatacaa ccaaacaact
     4741 tatgcaaagg acgctgcaat taggcgaata agggggaagg gaatgactac caattcagta
     4801 aatggttcca attcagcagg tggtttaggc ttgaatcaaa ttgattcagg taaaagacaa
     4861 aacttggggc aacaggattt tttgcgtttg atggtagcac aagttcaaaa tcaagatcct
     4921 ctgcaaccac aggcaaatgg ggagttttta tctcaactgg ctcagtttag caccaatgat
     4981 ggaatcacaa aaatgcaaga atcactccaa caattagctt catctctaca atctaatcaa
     5041 gcgcttcaag catccgcttt ggttggccgc aaggtgttag taaacagcaa taccctgagc
     5101 ctgggtacag aaggagatgt aaaagctgcc attgatatgg cacctggtgt ttctaatctt
     5161 cgtgccgcaa tctacacgga atctggcgag ttgataaaaa ctattccctt gggacaacca
     5221 gaacccgggt ttttccaatt tagttgggat ggtactgacc aaagtaatca acgtttagca
     5281 tcaggaagat atacaataaa ggttactggt acttatggcg gacaagaagt ggcattgaaa
     5341 actatgacgt cagccaatgt cgatagtgtt agtcttgggc aaaatggcga ggatctaaag
     5401 cttaatgtag cgggcgtagg atcagtctat ttaagtcagg taaggcaaat atcagtataa
     5461 atcaatgatt tcagtcaccg tggccttatt gaaacgatgg cttaagtcat aagcgcattg
     5521 cttataaagc agtcaagggc aaatgctaaa aaattataaa gcaggaggct attatggttt
     5581 ttaatacagc attaagtgga attcaggcat caaccagaga tttggaagtt ataggtaata
     5641 atattgcaaa ctctgccaca ataggattta aaggctcaag agccgagttt gcagatattt
     5701 attcatccag cgcttatggc tctggcagca acgctgtagg tggtggcgtt aaactgtcac
     5761 gtgtacatca atcattcgct acaggaacat taagtacctc aaacaatacg ctcgatctgg
     5821 ctgtgaatgg ttcgggtttc tttatattaa gtgatcaagg agccaagatt tatactcggg
     5881 ccgggcaatt caagttgaat aatgagaact atatagtcaa tgcaaataat caacggttga
     5941 ctggtttact aactgattct aatggggcaa ttacaggagc atcgggcgat ttaagaataa
     6001 acaccgcgaa tattaccccc agagccagtt caaatgtctc agtaggcgtt aatctgaatt
     6061 ctcaaagcaa agcgccgaat gtggattggg tcggaggggc tagtccggca accgatactt
     6121 ataacaatgt cacctcgtca acaatctatg acagtttagg aaattcacat gtattgagca
     6181 tgtattttat tcatgctgat tctactgcct tggctggttc acctaatgcc gcatcacctg
     6241 ctggcacaga aaatcaatgg tatgtagcat ttcagataga caatcaaaac gttcctccta
     6301 atgtgggggc tcaaaataca aataatttat tccgagctaa ctttaattct gatggctcgt
     6361 ttgcgggagt attcgatacc tcaaacgccg ctttacccaa taatttaatt cctttgtcat
     6421 tcaacttaaa caatggttcc agtcctttaa atctaaatat tgatttatcg gatagtacac
     6481 aatttggtag tccctttgct gttcagtcgg cttataataa tggctatact accggaagcc
     6541 ttgctggact ggatattgat gattcaggaa tgatctatgg tcgttatacg aatggccaat
     6601 ctttagcaat gggccagatt cagctggcta attttgcaga tccagaaagt ttgcaaaata
     6661 taggcaatac cagctgggct gaaactactg cttcaggaca gccattgatt agtgctcctg
     6721 ggagtggcgg tttgggatta attaattcag gaagacttga agagtcaaat gtggatttga
     6781 ctggtgaatt ggtcaagtta attaatgctc agcgtaattt tcaggcgaat gctcaaacaa
     6841 ttcgaaccgg tgatgcgatt actcaaacga tcatcaatat tcgctaggag gtaagtcatg
     6901 gaaccgatcc tgtataattc catttctggt agccgttcag attttaaaag acaggaaata
     6961 attgccaata atttggcaaa tattaatacc cctgggttta aggctgattt atatcaggca
     7021 caaaccatgt acatgaataa tgcgaatgga aataaccaat tcagcgctca ttcatttatc
     7081 actcaaaatg cgaacggaat tgatacttct cctggagaga ttattaccac aggcagagac
     7141 ttggatgtgg ccattgatgg ggacggatgg atggctgttc aggacagcca aggtcgtgaa
     7201 gcttatacca gagggggcag tttgcgtttg aatcctaatg ggcagttaat tactgcctca
     7261 ggcaaggttg ttttgggtga tggaggccca atttctatcc cgcctgctca atccatagag
     7321 attggttcgg atggaactat atcgattgtc cctttagaag gtgatgtcaa atctctggca
     7381 atcttagaca ggattaaatt agttacgctg gatagaaata atatctataa aaatgatgaa
     7441 ggtttgatcc aactgaaatc aggtgcggct actgctaata gtaatattaa attgcaaagc
     7501 ggtgcattgg aagggagtaa tgtaaaagcg attgatcaaa tggtagctat gattagtgct
     7561 ggtcgtgaat ttgatgccca aatgaagtta ttatcaacag tcgatgataa tggacaaaaa
     7621 ttggcacagc tattgcaaga gtaaacttaa ttaatttaat ttgtttcact gagggcgaac
     7681 cgttttccaa taattaggtg tcacaggtga ttttaggaac ggaaggcttt gataaactga
     7741 aatgataatt aaggccgata taataataag aataatagca ggaggccgtt atggaaccag
     7801 cattatgggt gagtaaatca ggtttggaag cccaggataa gaatattgct actattgcga
     7861 ataatcttgc taacgtcaac acaactgcgt ttaaaaaggg tagagcttta ttcgaggatt
     7921 taatttatca aaatcttcgc caggctggtg ctcaatcttc acaaaattcg caaattccta
     7981 ccggtataaa catggggaca ggagtgcatc tggttgctac agaaaaaata ttcagccagg
     8041 gcagtcttca gaatacacaa aatgcgttgg atgttgctat cgaaggacgt ggctttttta
     8101 cagtcttaat gccagatggc acacaatcct atacacgtga tggaagcttt aaaactgata
     8161 gtacaggcca gattgttaat agtaatgggt atcctcttca gcccaacatt acgataccgc
     8221 cacaaacaac caaaattact attgggacag atggcacggt tagtgcttta gtcgcaggga
     8281 ataatgcgcc atccgttata ggaaatatcc aattaaccga ttttataaac cctgcggggc
     8341 tgcaacccat tggtcagaat ttatatacgg aaactgtagc aagcggtaca ccgattacag
     8401 ataaccctgg caactccgga ttaggtactt tattgcaagg ttcattagaa gcttctaatg
     8461 tgaatgtggt tgaagaattg gttaatatga ttcaggctca aagaagctat gaaataacag
     8521 caaaaagcat tcagaccgtt gacagtatgt tgcaatattt aacccaaact ctgtaagtga
     8581 gataggttat gaaccgatta aatatagctg tctcttgctt agctacagcc ttgttgtttg
     8641 ggtgtgaagc cttacaccca ccggcaccag gcgacaatcc tgattatgct ccaacttatc
     8701 ctgtaactcc ggatcccaag gaattaagaa aggtaagtgg tgctatttat agtagtgaaa
     8761 cggctctccc tttgtttgaa acaccgaggg caaggcatcc gggtgatata ttaacggtgt
     8821 atttaatcga aaaaactgat gctcagaaaa atgcgactac cactcagaga aaaaatgata
     8881 cgacaaaaat cacaaataaa ctctttttgg gtagacccat ctctttggga agtggttaca
     8941 gcatggattt tgacttggat aaccaaaggc agtttaacgg agaaggcaga tcaatacaaa
     9001 acaataagtt agcgggcagt atatcagtga cggtagccaa agtgttggcg aatggcaata
     9061 tggttgttca aggtgagaaa tgggtcagaa ttaaccaggg caatgaattc gttcgtcttt
     9121 cagggatagt tcgtccccag gatataaaag cagataacac tattacctct gacagaattg
     9181 ccaatgcgag aatttcttat ggtggaactg gccaaattaa taatacgaat gctcaaggat
     9241 ggttatcaag gattttatgg gggcctttat tcccgactta attggtaatt atcgagatta
     9301 caaacgacaa ggaattaaga tgcggcgaat gctggttatt cgatggatat tggcaattca
     9361 tttgattgca actcaagttt ttgctgaacg aatcaaagac atcgctactt tagctggtgt
     9421 tcgtgtcaac caattggtcg ggtatgggtt ggtagtcggt ttaagcggta caggggataa
     9481 gactggcacg aaatttactg aagacagttt cgctaatatg ttgactcaac taggaattaa
     9541 cattcctcca ggagttagac tcaattccaa aaacattgca gcagtgatgg tcactgccaa
     9601 tttgtccagc tttatgaaaa aaggacagac tatggatgtc aacatttcat caataggcga
     9661 ttctaaaagc ttattaggtg gaactttatt acttactcct ttgaaaggag cggatggtcg
     9721 tgtctatgcg atgtcgcagg gaaacgttgt agtatctggt atcagtgctt ctgggagcga
     9781 tggttcaagt gtcactgtta acgtgccaag tggaggaaga attcctaatg gcgctacgat
     9841 tgaagctgat attcctaatc ctttttatta ctcaaactca ctgacttaca acttgcataa
     9901 cccggatttc actacagcga aacgaatgag tgatgctatc aatgagttaa tgggaccagg
     9961 cacagccaaa gctatagatg ctgggtctgt ggtagtaaca gctcctaaaa aattatccca
    10021 gcgcgtagat tatgtttcag tacttgagaa tattgaattt aaaccaggcg aagccatggc
    10081 taaaattatt attaatgcca ggactggaac agtggttatt tcaagcaatg tgattgttaa
    10141 atctgctgcc gtatctcatg gtaatttggt cgttagcatt acagagactc ccgttatcag
    10201 tcaacctaat gcctttgctt cagggcggac tgtgtcgacg cagcaatcac aagtcaatat
    10261 tcagcaaaaa aataaccgtg cctttatctt acccaagggt accactttga aagatatagt
    10321 cagagggatc aatgctgttg gtgcaacacc tgctgatgtg atttccatac tggaagcttt
    10381 acaacaggcg ggcgcattga gtgctacatt aatagtcata taaaggtgat ggatatgacc
    10441 atacaaagta ttgctacgag tgattttcaa gggttaaatg aattaaaaat tcaggcaaaa
    10501 aacaatgcaa aagaagcgtt gcccgaggta gctaaacagt ttgaaggaat attcttgcaa
    10561 tccatgttaa aaagcatgag gatggggcaa catttccttg atgaatcgag tccttttagt
    10621 ggtaagaatg aagcaacctt tcaagagatg ctggatgcac aatatgctag tactattgcc
    10681 gagtctaaag ggataggatt ggcagctctg ctggctaaac aactggaaaa ttcagttggg
    10741 gataaagcaa ataaaccagt taattcttct acagaggtca acaatactaa agtgactaat
    10801 tcagaagaat cgttatctgt tgttgatgat ttcgtaaaat ctgtgtggcc cacagcaaaa
    10861 caggctgcat cattaattgg attggatccc aagctgttgg tagcgcaagc ggcattggag
    10921 actggctggg gtagatttgt tacaagagat gcagatggtt caagcagtaa taatttattt
    10981 aatataaaaa ccgggtcgca tagcgaagta gagtcaattc aggttaaaac aacagaatac
    11041 attgccgata cacccattaa gatcaatgct tcctttagaa aatatccatc catagagcat
    11101 agttttcatg actatgtttc attaattaag ggaagtgagc gataccagat ggccttggcc
    11161 aatgcagaaa atcctgagat ttttgtcagt gagcttaata aggcagggta tgctactgac
    11221 ccaaattata gtaataagat tttgtctatt tatcatggtg atgaattgaa ccaggcaata
    11281 caacgatgtg aatcattcga acaaagttaa tagttaggcc caagttaaag aaaaaattag
    11341 ttcagggagt gaacaaaata tgtctatact taatattgcc tattcaggat tgaatgcttt
    11401 tcagcgtgcc ttagatgtta ctggcaataa tattgctaat tttaaaacca gagggtatag
    11461 cagacaatct atccaattta cacctattac ctcaaatcgc tatgctggtt catacatagg
    11521 agcaggggta tctgttgcat cgatttatcg aaacgttgat caatttgcca atgctcaggt
    11581 tagaagtacc ttgagttata gaactcagta tgatgcgttt tataatcagg caattcagat
    11641 agataaattg ctttcacagg atggatcaag tatttcggtt cctttacaaa ctttttttga
    11701 ttcaataggc caattgaaca gtactcccga taatatagcg acaagaggtg tggtactaaa
    11761 acagagtcag ttattagctc agcaatttaa ttcgctacaa acaaaacttg aagagtatga
    11821 gcgtaattca acattgcaac taacagagtc agtaaaaata attaatcgca taacaaaaga
    11881 gttggctgaa gtcaatggaa aattattagg gaataacaac atacctgaat tacttgacca
    11941 tagagacgag ctgctcaaac aattatctgg ttatactgat cttagtattt ttgatcaggg
    12001 tgatggtacg attagtgttg gaattgccag cggagatatg ctagtggcag gaacccagca
    12061 acgcgatttg ctagtaggta caggtaaaga ttctattttt ggaacaagga tctttttaag
    12121 tagtggagga aataaccaag tcgatattac agacaggctc actaccggta tgttaggcgg
    12181 tcttatagat tatgagaaaa atgttttggg acaggccagc caattaatag gtcaaatggc
    12241 cattggttta gctcagacat ttaataccca acataagttg ggaatggaca tgaatagcca
    12301 aattggcaag gattttttta ctgattttaa ttccccggga caaatgctga aacgatctac
    12361 agcttcagct gacaatagtg gaactgctgt cttatctgtc aatatctcag atatatccca
    12421 agtgaaatta agcgattacg atctgataat tagcgatact ggggctaatg aattgcgatt
    12481 gataagaaaa tcagatggta cttctacaac cttaacctgg tcaagctcgc caccagcccc
    12541 accagcaggg caagtagtta tagatggaat gacgattaca gtaaacgatt tatcgcaatt
    12601 ggcaaataat gaccattata ctctaacccc taccagagga gcagccagag attttgcctt
    12661 ggaaattaag gatgcttatg aaattgcgct ggcttctcca gtgaaaacaa cggcgtcatt
    12721 aaataatacc gggcaaggtc aaattatttt agggccagta ttgaatacgg cttctgttaa
    12781 caaacaattc aggatagatt tcatttcgga tactcaatac aacctggtta atgtaacgga
    12841 cagtacaaca gccggtccgt ttgcctttat accaaataca aacaatgtca ttcaaattcc
    12901 cgatggaata actccttctt attcagttgt tttatccgga attcccaaat ccggagacca
    12961 atttactact gattacaata ctggtggatt tggtgataat cgcaatggct tgatccttgg
    13021 caacatacaa caaaacaaga ttttttcaaa tggcagcgaa accttatttg atagatatgg
    13081 taatttgctt gctgaagtgg gaggcagaac caatcaagct aaaaccagct ttgaatctgc
    13141 cgatatactt cataagcagg cattggattt tcaggatagc aaaagtgggg tcaatctgga
    13201 tgaggaggga gccaatttgc ttgtctttca acaggcttat caggcagcgg gaaaattgat
    13261 ggaaatatcc aatcaaatta tgaacttgtt attcgatatc atgaggtgat gaatgcgtat
    13321 ttcaaccaat cagatatatc agcggggttt ggataatttg ttgacccaac aagagagatt
    13381 ggcaaggctt caggatcaac aagccagcgg cataagagtt cggactccat ctgatgatcc
    13441 agtggcatcg gcacaaattg agctgattaa acagcgaatt aacaacatag agctgttcca
    13501 aaataatcgt caagcagccg acagtgcctt acgtttagag gaagggatat tatccaatgc
    13561 agttaattca cttcatcgct tacgagaaat acaaattcag gctggtaatc ccagtctttc
    13621 cgaagaggat agaaaaaccc ttgctgttga agcccaggct ttattaaatc aattacttga
    13681 ttatgccaac accaaggaca gtaatggaag ttatatgttc agtggtagta aatcgctaac
    13741 acagccagtg tcattgaatc tctcgggaca atatgtttat aacggagata gcacacagcg
    13801 ttttcaagca gttacaacga gtttgctggt agcagttaat gataccggtg acaacgtatt
    13861 tatgcgtatt ccaagcggaa atgggcgttt tgccataagg gaaacgctaa cgcctaatac
    13921 aggaacagct tcagtcagtt ctggttccgt gactaatgaa gcagcttttg tgcctgataa
    13981 ttacacaatg acttttgctt taaattctca gggtaacctg gtagtcatgg tgagtggtac
    14041 tctcagtggt aatgtgatac ccccaagcgg tttaccggat gatgcaccat tatatcagga
    14101 gggtagtgcc attggtttta atggtatgga aatggtagta tctggcttgc ccaaagcagg
    14161 ggattcattt tcaattagtc cagcaaaaaa tgaatctatt ttctctactg tacagcgcat
    14221 gataaataac ttaaataaac cctatacctc ctctgtagaa aaagcagcta ctcaaactga
    14281 aaataatcaa ttgttagctc aaattgacag tgctttaggt cacattttga gcgtacagtc
    14341 tgatttgggc gcaaggctca atcaattgga aactgctgaa aaagcgaata atgattattt
    14401 ggatatcagt gctgcgacat taaaaaaact cagagaaatt gatcctgttg ctgttgcaac
    14461 ggaattgaat ttacaactag tcaatctcca ggcggctcag ttaagcttta cacgaattca
    14521 aggattatca ttatttaact ttttgtaaaa gcatatcaaa aatagagtat ttcatctttt
    14581 atactgagta gtgttgaata aactgatgcg aacttaacca ttgctgtaaa tcaatcaaca
    14641 taactataag ttataaccta taaaaagaac tcataagcat gtcacatcct gttcatattc
    14701 acctgttact atgtattaaa tacccaataa gcaggtgaaa ttatgaaaac ggtaaatagt
    14761 caaaatggta catcagcaga gccatctgta aatctaggaa aattgagtac tgcaaaaaac
    14821 ctcgtatttc gcggaggagg ctctaaaggg atagcttatg ttggagcatt gcaatccttg
    14881 tctgaacaag gggtattaga aaacattgaa aatgttgctg gaagctctgc gggtgcaatg
    14941 accgcagcga tagtcgcttg cggtggctca gccgatttaa tggaatattt ttgtagtgcg
    15001 aatagcttag aggattttgt tgataatcca ttaactagaa agttagcaaa attatttatg
    15061 gacaaaaaag gttttctagc tggaatatta atagaattat ttaaaagcaa taaaatgcta
    15121 ccatcagatt cttctgcagc ttatacttca ttaagtcatg agcaaacgga caagcagtta
    15181 gatgctaagt ccagtcttgc agaagcaaaa ctggtcacaa aaccttccaa gttaactttc
    15241 cctgttgctt ctaaaggaac caaattagtt gaagcgatgg agcatgtcat gaggatggct
    15301 attttcacgc attttcctga atctctggat aatgaattga aaaatgtccg cgagaagctc
    15361 aatgcaattg aggataaagg gagcgctgaa tatattaatc ttgcaaaaag acaacagatt
    15421 gtagaagaca cccaaaaaat cataaacgga ttgcatgatc cgaattttga tttaactttt
    15481 ggtcatttac acacgttaca tcaatacgat cctcagcgat tcaaggaatt gcacattacc
    15541 ggaacagatg aaaatggcga gttacggatt ttttctcatg agaaagatgc tgacatgcct
    15601 gtttctattg cagttcgtat ctctgcgtca ttgccaggag tttttgatcc ggtaatctat
    15661 gaaggaagaa aatatattga tggcggtgct gccaataatt taccagtcaa tatcttcttt
    15721 aataaggaag aagcttatac tccaggcgtt gtaactacaa ttggggtttc ttgcgagaat
    15781 caattggaca tcacagaaaa ggaatttaaa aatccatctt ttatggaaag gattaaggag
    15841 tatttaggag attttgttaa aaaaatatta tgtaaacttg atgttgaggg tgctgtaaaa
    15901 gaacagaaaa gagaaatgct gaaatacata gataatggaa atctctttgt tatggtggca
    15961 cctcccattt ccgtaacgac tgcgcaaatg catattaatg ataaagaaaa agagggcgtg
    16021 atcgaggtag gtaaagcatt ttgtgatatg caattgaaaa gtatgattga tgcagattat
    16081 aaaccacagc catttgatga aaaagcagca aaaatattga caaacagcag tcagcaacaa
    16141 gagcaattga aagaaaagac catgttattt aaatcaagat tgcaaagttt taaacaagga
    16201 gagggagtca aaccttggtc tcagcatgtg gaaaacgcta ttgatcgatt aatgtcactc
    16261 aaaggagaga ttactaaagc tcaagtagat ctgggtagaa catggtttga tataaaatca
    16321 gaaaatgccg acccggcagt tagattgaaa aaatttaacg acgcattcct tgcttctcca
    16381 ttggcgaaac caagcagtaa tcaacaggag attaatttca gtaaagaaat aagaaaggaa
    16441 attgatctat taaaaggtct gccaggatta aataacacct cttctcattg tacagaagag
    16501 tttaatgagc agagtacacc atccctatat agggcgtaaa agtttaatag gctgtgaaat
    16561 gtaaattaaa gcaagggtgc tttaggttac aatattttta aagctccgat ttattaaatt
    16621 gtataatctt gtaagtttct ttggaataga ccctttaata aatggcgtcc cgggcaggat
    16681 tcgaacctgc gacctgcccc ttaggagggg gatttaataa atatcccgat atatctccat
    16741 gtgtctctat gcaaatcaat ttcttataaa taaagggttt tattgctttt tcattacatg
    16801 gaaatacatt gttatataac catacgcaat taatttgcac cctatatgca cccttatggg
    16861 aaaagtatat actgcttcta taatttttta aaacgaagaa tacaataata atttatggat
    16921 agagagcagt tatgaacgaa ttatcacttc aagatttacc agaataccag agctttttag
    16981 tcgaagctat gcaacagatc actcaatctc gtattaaagc tgcttattcg gtcaatagag
    17041 aactgataat gctttattgg tggcttggag agcatattgt gactcaacaa aatacctatg
    17101 gttggggaaa atcattcgta gaaagatttt ctttggattt aaaaaagcac tatcctgatg
    17161 ccaagtttgg cttctctcca caaaacttat ggtatatgcg tcagttttac ctggaatata
    17221 aagataagcc aaatctccaa cagcttgttg gagaaattcc ttggagtcag aatttactta
    17281 ttttaagcaa gataaaggat gacaatgaaa agttatatta tttaactgcc gcgaaagaac
    17341 aatcttggag tagggaaaca ttaagagaac aaataaactc acaagcttat cagcggcatt
    17401 gtttatcgga gaaaaaccat aactttaaaa acacattacc tcgagattta gcagctcaag
    17461 ctcaacaatc aatgaaagat gtgtatatgt tcgacatgct tggtattact gaaccagtag
    17521 ttgaggctga aattgaacgt agaatggtag aaaagattaa agatgttatt ttagagctcg
    17581 gctatggttt tagctttatc ggtaatcaat accgcattgt tacaccagat tcagaatact
    17641 ttattgattt actattctat caccgcaaac tgcaagcact tgttgcacta gagttaaaac
    17701 gatcaaaatt taagccggaa tatgctggta aaatgaattt ctatttaaat ttgttagatg
    17761 attttgttaa agaacctcat gaaaatccat ctattggtat tattttatgt ggtgatcata
    17821 gcaagtttga tgtggagtat gcgttacgtg gcattgataa acctgttgga gtagcagggt
    17881 atcaattaac acgtgatata cctaaacagt tgagagatgc tctaccagat gcagatcaat
    17941 tagaagaaaa aatcatgttt gagttggggc taaataatga aagccaaaat taataacacc
    18001 ctgctaaaaa aattagttcc tcaagacaaa gaatatgagg tacacgacac cgatttaaaa
    18061 ggatttattc ttagagtctt tccctctgga acaatgcgct atgtctgtca gtacaagcga
    18121 ggcggcaaga ttaatattgg gactgtggga gtgatgactc cagctcaggc tagagaaaaa
    18181 gcagtcgaaa ttttaaatga ctttaataaa ggcattgatc caaaagcgaa aagaggaagt
    18241 aataaactta aaagcctagg cgagttcttt gaaaatgaat atacgccttg ggttttaact
    18301 catcataaaa gaggggacaa aacgctcgct acaattagac gctgttttga taaattattt
    18361 tccaagcctt tggaagaaat aacaccttcg attatagagc aatggcgcat caaacgatta
    18421 aatgatggta tttctaatgc aacacttaat cgagatattg gtacattaaa atctttattt
    18481 actaaagcgg tagaatgggg atttattaaa gagaactact taaaaaattt gaaactttct
    18541 aaaattgata gagcacccaa agtgcgttat ttatctattg atgaggaaag aaatttaagg
    18601 cgagccctat ttgagcgaga agggaagtta aagcaagatc ggaaaagagg taatgagtgg
    18661 aggcaagcaa gaggatatga tttgctgccg gagtatgaag aaaatgaact ttgtgactat
    18721 ctgacaccaa tggttttgat ttcaattaat actggattga ggcaaggtga gttatttcat
    18781 ttaacgtggg aaatgattga tttacaagag cgatccatca ttattgctgg tcagattaca
    18841 aaaaatagca gttcgcgcta tatacctcta aataatgaag tttatggaat aataaagcaa
    18901 ctttatgaaa aaagtaattc aaagaagggg ctggtatttc tcagtaaaga caataagcct
    18961 tttaataata ttaaacgatc ttggacaaca atcttaaaaa aggcacaaat aactgaattt
    19021 cgctggcatg atttacggca tcatttcgct agcaaacttg ttatggcggg tattgattta
    19081 aatactgtgc gagagctatt aggtcattcg gatataaaaa tgacattaag atatgcacat
    19141 ctagcacctg agcataaaat taaagcagtt aataagatca attggactga ttaaacttac
    19201 atttattaaa tgctttccag tcttcgacac cttggttcga agcctgatac tctaatttta
    19261 aattcgttat aaatcaataa ctagtgaagc gtcaaactca cttgtaactg cctagaacgg
    19321 cctattacag tatagtgctg tcacagttga gacacgtttt gattgcagaa gactatcaac
    19381 cattttgata atgcggttca ttgttaggaa atagatatct ttatcttaat atttaaattt
    19441 taatgatagt agaaaaatac aacaatcttt aatttttaat tagtcacata agatcaatta
    19501 caaagtgcaa aggtttaagt aaattaggct attctatcga tagacaatga aaagaaagag
    19561 atactaaact taaaaaacag ctgagaatga tatgtgcggt cgttttgctt atattgcttc
    19621 ttatgacaaa ctgaaatacc aatttcatct agccaatgcg attgaagttc cacctcgctt
    19681 caatatttct ccaggagctg atgtattatg tcttgttgaa accgatggtc ataaaattca
    19741 atgcgtatta ttgcgttggg gattcattcc ctcgtgggca acggacagaa aaaaattagg
    19801 aaatctcatc aatgcacgag ccgaaacagt ttttgaaaaa ccgacttttc gtcagtcaat
    19861 aaaatcgaaa cgatgtttaa tccctatgag tggcttttat gaatggcgcc aagaggatgg
    19921 tgtaaaacag ccttattttt ttcaaaaaaa gaatcatgat ttgctggctg ttgctgctat
    19981 tcgagatacc tggcagcaga gcgatgaggt gattcattcc tgttgcctga ttacaacgga
    20041 tgctaatgca tttatgcaac cagtgcacaa tcgcatgcca gtaattttag gtgaagaggc
    20101 tcaagctatt tggcttaaca acacgcaata tgataaagca caattaatgg cgttaatgaa
    20161 accttatcct tatgaggatt tggaaggcta tcgagtgact actttagtga acaaagccaa
    20221 ttttgaccat cccttggcga tggagccatt atctgaataa aaaaatttaa aaataatttt
    20281 cctaatattc tcgaaggctt agtgtggttt tcatttccag ttgttctgag atgggattta
    20341 cagtttattt ttccttgtct atgatatttg tacgtaaatc aaaaaaagaa tcatcatgtc
    20401 accacgaggc ggaaaaagag taggggcggg tcgcccaagg ggtgagccta ccaaagcagt
    20461 tcgtatatca ttatcccaac ttgctgaatt agagcgtcta aagagtcacg catcgtatca
    20521 attgccggta tttgctagca aaattcaagc aggatttcca tcaccggccg atgattacat
    20581 cgaggggtat cttgatttaa acaccaaatt cattaaacac ccatcgtcta cctttgtttt
    20641 gcaagccaca ggggagtcta tggtggaggc tggaattttt tctggcgact ggctgttagt
    20701 ggatagaagc atagaacctt cggatggacg cattgtcatt gcagctgtga acggtgaact
    20761 cacggtgaaa cgcttgtcga aaaaaggggg aagggtgcaa ttacttcctg ctaatcccaa
    20821 atttcaaccc attgacatta cagaggacag tgagatggtg atttggggag tggtgacttt
    20881 ggttctacat gagcttgcct gagatgtttg cccttatcga ttgcaacaat ttttacgcca
    20941 gttgtgagcg tttgtttcgt cctgatttaa aggacgtccc catcgtggtg ctatccaata
    21001 acgatggctg ttgtatcgca cgctcgaatg aagccaaagc attgggcatt gccatgggcg
    21061 agccgtactt caaaattaaa catttgtgca aacagcatgg agtgaaagct ttttcctcaa
    21121 attatacgct gtatggcaac atgagtcatc gtgtgatgtg cactattgaa gaagcctggc
    21181 cccatataga agtttactcg attgatgaag cgtttcttga tttaaggagt ttaccggttg
    21241 atagctatga ttcgttttgc gagcagttac aaaagaaaat cttgaagcac acaggaatac
    21301 ccacttccat cggtattgga cctactaaaa cactagctaa agccgccaat catttatgca
    21361 aaaaagttta taaaattcct gtgtttaata tcacctcgaa tcgtgggcgg ttattgcaac
    21421 agatttccgt tggggacatt tggggagtag ggcggcaatg ggccaataaa ttaatttcgc
    21481 gaggcattca tacggcttat gatttggcaa tgaccaatcc tcaccttctg aagaaatgtt
    21541 ttaacgccgt gttgatgcga actgctatgg agcttcaagg aattgcttgt ggcggtttag
    21601 aggtaataga gcctaagcaa agtattatgt catccaaaag ttttggtcag atgcaaactc
    21661 aaattgcttc gattgaggaa tcaatcagta gccattgtgc tcgtgcggtg gagaaaatgc
    21721 gtcgccaaca attggtggcg acgcgtctgg ttgtatttgt gcatacgaac cgatttcggg
    21781 aagatttggc tcagcacttt cagtccatcg aatttaagct gattaatcct acagatgatt
    21841 tgcgcttaat taccaaaata gccaaaagat gtctgcaacg catttttaaa ccagggtatt
    21901 actataaaaa ggcaggggta tgtcttgaag acttaattcc taaaaaacca cgacagctgg
    21961 atatgtttca tcaaccaagt gatgagcatc taaaacacac cgaacaattg atgggtgtct
    22021 ttgaccaaat caatcaaaag tatggaagaa gcacgattcg gttagccgcc gaaggctatt
    22081 caaaaccctg ggagatgcgt gctgagctga aatcacctgc ttataccacg cgatggtctc
    22141 aagttcccaa agttcatttg tattaagaaa actcacaaaa taactcagtt tttggtgttc
    22201 accttcccaa gaatacacaa tagcaagaac atgcttactt aaaggctcta aaactttgga
    22261 tcgagcaatc ttgtcaactt gtttgcagta ggtaaattat ggtgtatttt gatatggtct
    22321 attattgcct gaaactgtct agtactgcgt tagttatatt taaaactgtt acaaaaatgc
    22381 tacagtgaag tgtttaaaaa ggccgcggga atcgaacccg ttaccatggg ttttcgatat
    22441 ttaatgaaat acaactaaag accatgaaaa accatacaaa ttctgtgtta tcgtactttc
    22501 acaaaaatac atcaagttgt ctttcatggt ctagaattgt ctgaaaatag tgctgttttg
    22561 gtgccgataa atgcatcgta ttcaaacagc cttattttta aaaatccgac gcgtttagag
    22621 tcaatgattt tattatagga aaaaaataat aggttacaaa attcatgatt ccaatacaaa
    22681 taacgctgct tcctaaggag aatctatgca aaaaatactt gggaaattga cttaaagaga
    22741 acacttagtt taaaaggcta ttagtaaaat attaagtgtt cactttagct cagaatataa
    22801 cgtattaaaa ggctttttct gcataatgga gagataaggg atacttgatg ttgttttatt
    22861 aatttttact acgtttgggt tggatagatc aaggctcact gttcctagtt gcaagcaatc
    22921 aaatccatcc cgacatgcgc tagattctcc tatatctata atacattctg atgttgagcc
    22981 acataagtca tataattcat tatatgacaa aatagatgag gatttatatt taggctccca
    23041 ttcagaatca ttcccactgg cgaaataaaa taccatgtca aagctttttg aaccatttga
    23101 agttacagtt atttcaggtt cctctgttaa cggacaatgt tttggatcat ttgaagaaca
    23161 cgacattcca aaattagtac ctgtccagct agtcccctct gcaagaaatg gattaaattt
    23221 gttaatatat ctagtggtgg tggttggata attactatta ggatcgttca aacttatatc
    23281 taatggtgct ggttgtttat catttaaata atatttgcat acgggacgtt tttcagctga
    23341 aacctcgact cttttaaatt tataaattcc ttccagaagt tttggtcctg aaaacgtata
    23401 gcctccccat acctctgagg ggttatccat aacgcggcaa cgttctgtgc catacaattt
    23461 aaaatcacat ttaacgctat tagggcaata caatataggt tggccatcag gagtcgattc
    23521 ggaataaaca ggcagcgata gcgaatataa acaaatagcg gatgtgataa tttttttcat
    23581 ttatagcaac aaaaacaacc atttcggaaa ataatatggc atttatttgt tcaagtcaat
    23641 tttcttttgg gaaacagctt aatttttaat accccgcgct tattacttct attcatagag
    23701 gcaatataag ttaggagaaa cacacttagc ctgttgaatt ttaattttaa gactaagtgc
    23761 ctgaaaaacc atagttctca tatctccgta aacaactttc aactataact ctgcctttgc
    23821 taacggctcc cataacgtgg gttctaatga tgtctctaaa ccaccaacta tggatctaat
    23881 ttttctttca tttggaataa gcaaatctag aatttgaaat tttcggtttt actttaatga
    23941 tctcctagca tatccctcta tatttttcac gatagttgat ttaactattc ttttaaactc
    24001 ttaatttttc aattttaaat tctctgattt ctcataatta ttcatgttat taactgacta
    24061 taaccctgtt atgaaaaaat ctcacataat atggataagc taccttgaaa taatcgatta
    24121 gctctttgag aattcactat taagccaggc caaaacagca tcggttgaat taattccctt
    24181 tttgattaaa attctagaaa tatctcctcc agataaaaaa actataggat gaccatcctc
    24241 tctaacctct ccataagctt gtttcgcaat aaaagaggtt gtaaccaaaa ccccaaactg
    24301 tctatttttt attctagaaa ttagtcttga tacttcttta accccaacag aatttatatt
    24361 ttgaccattt aaacctggtt gataacattt agcttctaaa aaaaattcag catatacagg
    24421 atcttcctta atgccaagaa cataacgccc aattgcatcc tttcccccat ctactgcgct
    24481 tcgagttatt tcatcaataa gtacattttc atcataaagt tggaatatct ttgcagcaca
    24541 agcttcaaat tttataggtg cctcacaaaa ataatcaaat acagcttgca atatttctct
    24601 ttcaagttta gttctgggca tttgctctat ttgagttcgt atcgttttag tactaggtgc
    24661 aatcaataca tcagctttcc cactaatttt ccattgatag aatggattaa gagaattatc
    24721 ttggccaaag ggatcgaaca aagaattaat ccatttccta gaaaccatag ggatattgag
    24781 aatcgtaaaa atagctctat aattttggaa acgttgccca tttgttgttt tccaaactgc
    24841 tataagatcg tcggtagcag atagcccggg atacccagga actgcaactc ctttaaattg
    24901 cactgatcta gaactactcg ctgtaggata cttaacaaaa ataaatatag ggggaacaat
    24961 tcgccttgca tctttttcgt tgtgtgttga gtcaaaaagc atttttaatg ttgcattccc
    25021 ctggcgaggg gtatcatgaa tatcatgtcc aggatgttta ttgtctccat aatagataaa
    25081 ttgtccttta gatgtgtcta tagaatcagg ccaatcacca tcttccatat ttgtatataa
    25141 tacaatcaat tttttaaata ttccttgacc tgctgaccga aaccctccca tattacctat
    25201 tcctttaatt atttttgaaa taggatcatc acttgcattt cctgagcttc caccttcata
    25261 aactgcatca ataatcaaat cagcattagc aagagtatca aatgagtata ttttcatatt
    25321 tcctttatgc ttatttaga
//
LOCUS       DACTJE010000028        25169 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.028, whole genome shotgun sequence.
ACCESSION   DACTJE010000028 DACTJE010000000
VERSION     DACTJE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25169
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.028"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            434..2065
                     /locus_tag="I9760_000889"
     CDS             434..2065
                     /locus_tag="I9760_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HAT5042668.1"
                     /translation="MHPNYYLSPLAVAIALGIASPVKAADPIPLQKSSFSEVTQKFQL
                     TLPGVMKGAVVSTNSLQFIRQHTDGNKVTHVRMQQQYAGFPVFGGYAILHSKNATPSL
                     ATAKSDVKMNGVIYDGLQAELGQPKPSFVKNASLALQQFKDKYANKQISEDQVTPMIY
                     IDEKHQAHWAYKVSVLVIHDDRIPERPTAIIDAETNKPFVQWDDVKTEKVQAKGMGFG
                     GNRKIGEYQFGKDLPLLEITRDSSVEMCFMENTDVKVVDMGHKYYSNNKPMQFTCKET
                     PDTQSTKTYYTGYSADGYDRDNGAASPTNDALYAGYVIKHMYHDWYGVEALTKSDGSP
                     MQLVMRVHYGQGYENAYWDGKQMTFGDGDTMMYPLVSLGVGGHEISHGFTEQHSGLEY
                     FGQSGGMNESFSDMAAQAAEYYSVGKNSWQIGPEIMKEDSGYDALRYMDKPSRDGMSI
                     DVADDYYGGLDVHYSSGVYNHLFYILANQPNWNLRMAFDVMVKANMDYWTPYSTFDEG
                     GCGMLSAAKDLGYNLDDVKKSLSEVTINYQSCYVD"
     gene            complement(2067..2915)
                     /locus_tag="I9760_000890"
     CDS             complement(2067..2915)
                     /locus_tag="I9760_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5042669.1"
                     /translation="MKTKILSIPGLSIACKVWGNPDNPPILALHGWLDNANSFDNIAE
                     HLQNDYYFIAVDLPGHGHSSHLPPGCIYHFTDGIFTVVEIINALGLNKLHLLGHSMGA
                     CLGSLVAGVAPDRFLSLSLIEGLGPFSHPAETACQQLSKYLDYLSQKQSKKAKGYNKF
                     EHAALARSVKGYVSLDIAKSLCERGIQQENGLYYWRHDRRLLAPSPLQMTEAQVLSCL
                     TEIKAQTYLIWASKGFSFDSDKMKARIQAVKNIKIERLDGGHHIHMEKPEVISRLLAK
                     FYQSIK"
     gene            3383..4138
                     /locus_tag="I9760_000891"
     CDS             3383..4138
                     /locus_tag="I9760_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EEP domain-containing protein"
                     /protein_id="HAT5042670.1"
                     /translation="MQPEVRSVSLITYNIHKGFGVGAVRFLLPEMRDAIAGLNPDFVF
                     LQEVQGKHRKREKRIVSWPDSPQCEYIAENIWPHYVYAKNAVYQSGHHGNAILSKYPF
                     ESFESINLSNMNRASRGILHTQLKLDNNIIHLLCVHLGLFKAERTEQCKALIQRIKDV
                     VPKNEPLIMAGDFNDWRHVISKILAENLGIEEAFVALEGQHARSFPAIKPALCVDRVY
                     FRGMKVQEVACLQGKPWRMLSDHLPLYARFELL"
     gene            complement(4316..5326)
                     /locus_tag="I9760_000892"
     CDS             complement(4316..5326)
                     /locus_tag="I9760_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class I"
                     /protein_id="HAT5042671.1"
                     /translation="MSYEELSNTMEQMLHEGKGILAADESNSTIGKRFASINTENTEE
                     NRRDYRLLLATTPDLEQYINGVILFEETFTQTDEHGTPIPELFANKGIIPGIKVDKGL
                     VDLANTEHEKVTQGLDGLTERLLHFKKLGAKFAKWRNVYSISEYTPSLTAIKTGAENL
                     GRYAAACQSVGIVPIVEPEVLMDGNHSIEHCAEVCEMVLHELFHTLFIHQVELEHIIL
                     KPSMVTCGKEVAPFSDPEEVADYTISVFRNNVPASVPTINFLSGGQTPQQATANLNAI
                     NQIGHQPWLLSFSFGRALQEDCLKEWCGNKANIAKAQEALIKRARLNSMACLGEYQND
                     ME"
     gene            complement(5476..6210)
                     /locus_tag="I9760_000893"
     CDS             complement(5476..6210)
                     /locus_tag="I9760_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HAT5042672.1"
                     /translation="MQINTFPITLEESFMISPKVKHFIFNCELSPPFEYLPGQFITIH
                     FEHDGKNLKRSYSIANEPKKDNRIEFAAGYFEGGPGTELLYNLKPGDVIHINGPFGRL
                     IFRDETPGRYILVATSTGTTPYRAMLNELGQRIEKHPDLQVVILQGVQRSEEILYPDD
                     FQAFAKKYPQASFRPYLSRVQKQDLKDNEYSGYVQHAFPELNLNPTRDMVYLCGNPGM
                     IDEAFNYLKEHGFSMQHIVREKYISR"
     gene            6294..6836
                     /locus_tag="I9760_000894"
     CDS             6294..6836
                     /locus_tag="I9760_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmC"
                     /protein_id="HAT5042673.1"
                     /translation="MNTIDLITILGNISQSLYPVQKLITGGAYILGVLFFLTAIGKLK
                     KIGDHRAQSSSQEKMFTPMMYLLVGALLIYLPSGMDLMANTAFGVGNVLTYSNYNPAN
                     IYSSMGFLIRTAGVLWFIRGCVLVAHSSQPGTQQGLKGLVFLIAGVFAMNFDNTIAMI
                     NSALDYFVKATLSVKAAAGF"
     gene            complement(6980..7264)
                     /locus_tag="I9760_000895"
     CDS             complement(6980..7264)
                     /locus_tag="I9760_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HAT5042674.1"
                     /translation="MSNRKNKTIVIEGVTSQGKTFRPSDWAERMSGSLAVFKNSRIYY
                     SPLLQPSVNSEGYKCVLLDPKLKESSPQVYQAIMDFAKANNLKICGEEDL"
     gene            7494..8231
                     /gene="pssA"
                     /locus_tag="I9760_000896"
     CDS             7494..8231
                     /gene="pssA"
                     /locus_tag="I9760_000896"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAT5042675.1"
                     /translation="MNKENHSGIYLLPNLLTTVSLFAAFYSIVASTKGQYEAAIVAMF
                     IGMIADSLDGRIARLTNTQTEFGAQYDSLSDMVTFGVAPSLLLYNLTLHQLGKFGWLV
                     AFIYTAAVALRLARFNTQIGKSDKRYFQGLPCPPAAAVMASFAWICFQNEWANLIVSL
                     FTAVLALIVSVLMVSNLRYYSFKEVDYKGKVPFLYVLVMILLFVAIAANPAIVLFASS
                     GIYALSGPMLTVISLHKMRKQRRNMSK"
     gene            complement(8473..8742)
                     /locus_tag="I9760_000897"
     CDS             complement(8473..8742)
                     /locus_tag="I9760_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAT5042676.1"
                     /translation="MIKTKIKIINRLGLHARASAKFVSTAAKFQSHIDVTKDTQTVNG
                     KSIMGVMMLAANKGSELTLEIDGPDEERMNEALVELINNRFGESE"
     gene            complement(8933..9232)
                     /gene="hpf"
                     /locus_tag="I9760_000898"
     CDS             complement(8933..9232)
                     /gene="hpf"
                     /locus_tag="I9760_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAT5042677.1"
                     /translation="MQINITGHGMDVTPALRAFTEEKFDKLERHFDHIRAINVVFDVE
                     KVRQIAEATVLVTKGELHASSESEDMYAAIDTLVDKLNRQLIKHKEKLLAHRDRD"
     gene            complement(9259..10653)
                     /locus_tag="I9760_000899"
     CDS             complement(9259..10653)
                     /locus_tag="I9760_000899"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAT5042678.1"
                     /translation="MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESN
                     PLLEATPVEEKEDAQINTNQNQDEYSDFQWSQLYNNLSKRNNFEENDYNFDNLHCTTT
                     NLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPVAEIHASLNSDDFPLDIEE
                     IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNY
                     RQLMKNHQISEKTIHEILQVIHKLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLN
                     QDILPRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQSRQETLLKVATCIM
                     EYQHGFLEFGEEAMKPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSH
                     VNTDSGGECSSTAIRAVIKKLISAENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREA
                     MGIPPSNERKVISS"
     gene            complement(10864..11028)
                     /gene="rpmG"
                     /locus_tag="I9760_000900"
     CDS             complement(10864..11028)
                     /gene="rpmG"
                     /locus_tag="I9760_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAT5042679.1"
                     /translation="MAAVTIKVKMESTAGTGYYKTTTKNPRNHPEKMELMMYDPKVRK
                     HVLFKEKKVK"
     gene            complement(11043..11279)
                     /gene="rpmB"
                     /locus_tag="I9760_000901"
     CDS             complement(11043..11279)
                     /gene="rpmB"
                     /locus_tag="I9760_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAT5042680.1"
                     /translation="MSRVCQVTGKRPMVGHKVSHANNKTKRRFLPNIQNHKFWVEEEN
                     RFVTLRLSTKGMRIIDKLGIKAVLDKIRAKGEKI"
     gene            11548..12225
                     /gene="trmB"
                     /locus_tag="I9760_000902"
     CDS             11548..12225
                     /gene="trmB"
                     /locus_tag="I9760_000902"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAT5042681.1"
                     /translation="MQRKIKSYVLRAGRISNRQQQGLDLWLEDYELKFDSPSPWNFAK
                     EFGRHDADTIVEIGFGMGTSLFTMAMNNPQCNYLGIEVHKAGVGSLVADLHEHQITNV
                     RVVVHDAVDVLQTKIPENSLAGVQIFFPDPWHKKRHHKRRLIQSEFIQMLVKKIRPSG
                     FIHCATDWEDYAEHILNVLSSESALFNQQKEGGYSPRPDSRPLTKFEQRGERLGHGVW
                     DLVFIKK"
     gene            12237..13361
                     /locus_tag="I9760_000903"
     CDS             12237..13361
                     /locus_tag="I9760_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAT5042682.1"
                     /translation="MIKHAILISGLIVSFLASAAEMSFLAQLTEVPGASGYEKNVRHL
                     VQKQWQSYMAELKIDGMGNLIGQRKDNQKGPKLLFMAHLDETGFMVESITPDGFLKVI
                     PLGGIANSVIYAQRWTVSTPKGPVLAYSGMDSPHLLGDKKMSGSPDINALFLDIGAEN
                     KEQAQKEFAIQPGLEVTPVSEFSQLSKNRFLGKALDDRLGLAVISDALRAVKQQPNQL
                     YLAATVQEEVGMRGASTVYKATHPDIAINVEVGIADDYPMLLADRKGRIGLGKGPSLF
                     VYDKSMIPNQELINWIMELANKNNIPLQLEVEPGYGEDGSKVQASGMGVPVVNIGIPI
                     RYAHQHAGIFDKRDYDNTVKLLRLIAENLNQEVVDKIKKS"
     gene            13439..14926
                     /gene="ankC"
                     /locus_tag="I9760_000904"
     CDS             13439..14926
                     /gene="ankC"
                     /locus_tag="I9760_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkC/LegA12"
                     /protein_id="HAT5042683.1"
                     /translation="MDFVSEMNKILQMELEQFKEFIQKKLEEDKNYLEKLFWLPNGSQ
                     MTVLNYLIEQYSEPKLGIDDANRDLLAKIDFVLHEAKDVNVGEPLHQAIVAGKISLAL
                     HLLGVDENNFTPGESEKTDVLSILSKVKKKIEESFNHVKRCFFDVDKRDGYGRTLLSL
                     ALDAKRQELLIAILARNPIVHATTLRSSAYVPFQPIHQAVVLDYAEGITLLASMGAQL
                     TNPLGSMRDTPVILAARLGKINALAALLELPTQSLSLESENNHLFEDKQTGHTAVEEL
                     CERMANENDKAEALRGIAMLICRGAEPPRNEKMRNLLSSNRVAFLKAVSTYLADKPQL
                     VDAFVERCHLRESALHNIVYADHSWGSSIRHLLGVPSEAALIVEELVTRKYSDPSFAS
                     ENVSSLSTTATENLRSERNPLKLYAEFVRRYTQAYDSQMITNRWSTMRWMIAEGNCDW
                     DTVVRYSKNHPTSRTRIVLNEMFNPIPRVHEDIESQQNSPRVVLK"
     gene            15135..16277
                     /gene="hflK"
                     /locus_tag="I9760_000905"
     CDS             15135..16277
                     /gene="hflK"
                     /locus_tag="I9760_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAT5042684.1"
                     /translation="MGWNEPEKGKDPWSGKNQPPDLDEALKRIQDKLKKTFFGGTGKS
                     NNNSSGGSSGGLLAVTVLLIAFILWALSGIFIVDPAEQAVILRFGKYAETVGPGPHWI
                     PRFISSKIVMNVDRVLDYSYSAQMLTSDENLVSVSLAVQYRINDLSEYLFNVANPEES
                     LQQATSSALRQVVGTTTLDQIITEGREVWGGRVQETLTKTLESYKTGILIVNVSPQPA
                     RAPESVQDAFDDAIKAQEDEKRFKEQAYAYAAKVVPIAEGKASRIQQEAEAYSKQVVL
                     RAQGEVAEFLALLPQYNAAPQVTAKRMYLEAMQKVMNKSSTIIVDSKAGNLLYLPLDK
                     LLGNQYVPQSGNLKAIKNGTNVSNEEDNLILSGRDLTRPTYSQGRD"
     gene            16280..17194
                     /gene="hflC"
                     /locus_tag="I9760_000906"
     CDS             16280..17194
                     /gene="hflC"
                     /locus_tag="I9760_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAT5042685.1"
                     /translation="MNAFKTTLGVLIFLVLVLLFTTMFTVTQGQQGIILRLGRLVKDP
                     QTDAVKVLNPGLHFKTPFIESVRIFDTRIQTMDIKSTRIVTKEKKDVMVDYYVKWRIS
                     DLAQYFKSTGGNEFKAETLLEQQLNTLLRAQFGKRTISDAVSGGRDDVMEILRNAAEK
                     QAGELGIKVVDVRIKGIELPSNTSNAIYQRMRADMQKIANRHRADGQAAAEQIQAKAD
                     ADVTVLLAKTNSNAQRIRAVGEAEAAAIYSKAYTQNPDFFALYKSLLAYEASFHSKKD
                     ILILDQSSSFFDYFKQAMPKNDGTAAKK"
     gene            17329..18624
                     /locus_tag="I9760_000907"
     CDS             17329..18624
                     /locus_tag="I9760_000907"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAT5042686.1"
                     /translation="MGKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTL
                     KINGVKTVLRLIPSGMLRPNVTCYIANGVVLSPQALLSEIKELEGNGVNVRERLRISL
                     ACPLILPYHIALDKARETHMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFAN
                     KLTELLDYHNFVLTQYFKQPAVDLESLLDESLQWAEELRPMVCDVSACLHEHRKQGEN
                     ILFEGAQGVYLDIDHGTYPYVTSSNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGG
                     GPFPTELLDDVGKRIAERGQEFGAVTGRPRRCGWFDAVLLKRSIELNSISGLCVTKLD
                     VLDGLEVLRIAVAYKDRDGNILSRPPLAADDFNDLLPVYEELPGWQESTADVTVMSDL
                     PANARAYLKRIEEILGIPIDMLSTGPERDSTITLRDPFL"
     gene            complement(18798..19545)
                     /locus_tag="I9760_000908"
                     /pseudo
     CDS             complement(18798..19545)
                     /locus_tag="I9760_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212725.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(19750..20079)
                     /locus_tag="I9760_000909"
     CDS             complement(19750..20079)
                     /locus_tag="I9760_000909"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT5042687.1"
                     /translation="MQGNDVNKEARFAFLTLDYALHAGSDALVFNGQRGAQLMLKRLR
                     TPEELGMISPHQLRYTFCKNLVNADISLEKMASLAGQERLDTTKLYCQSTFFDLSEAV
                     ERIGEMD"
     gene            complement(20057..20251)
                     /locus_tag="I9760_000910"
     CDS             complement(20057..20251)
                     /locus_tag="I9760_000910"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT5042688.1"
                     /translation="MNRNQQNQLLNHFEQNGRRRDIAIVKILLNTGLRASELCNLKWS
                     HIVLNERKGHLTINAGKRCK"
     gene            20418..21584
                     /locus_tag="I9760_000911"
     CDS             20418..21584
                     /locus_tag="I9760_000911"
                     /inference="COORDINATES: protein motif:HMM:NF012772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5042689.1"
                     /translation="MINNALICTKNNGLATLVASVCQYCGFKDLCVKNAREISNILEN
                     ESIKLIILDTALQEPCIIDVLYILSNLKCEIPIIALGECDDKILLSIKRIGELKKLYV
                     YTMELNSFSEEAFCEILNLIDKKSQIINEEAITIALEKKQFFMHYQPKISLSTYKLVG
                     VEALIRWQRPNHGLVQPDSFIPVAEKTGLIIPMTYWVIQDVFRQYSAWKRKGIQLKFA
                     INLSPNILTNVILPDELGKLVKEYKICPEDICFEITEAAAMHWPDIVLEVLTRIRLKG
                     FSLSIDDFGTGYSSLVELQRLPFTELKIDKSFVSDLAYNKSNMHIVKSIIGLGKNMNL
                     TLIAEGVETKEVADKLLAMGCENMQGYLISRPLSESEFLNWYRNKIDKNEIFTA"
     gene            21595..22317
                     /locus_tag="I9760_000912"
     CDS             21595..22317
                     /locus_tag="I9760_000912"
                     /inference="COORDINATES: protein motif:HMM:NF012301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT5042690.1"
                     /translation="MRTIKVQGSKPTILIADDDLSTRMLLRATISQWDYPVIEASDGE
                     EAWEILKQNDGPQIATVDWMMPKLDGLDLCRRTKNLPRPPYMILLTSMSGASNVVNAL
                     DSGADDFLTKPFNFIELRSRLHVGQRIINYANRLESVINEKKPHENISQHLPITIKRL
                     NAMGKKILDEWINLQSMLHLLEAMEKDNHIKFKNQILGIYERQKELIEEITSLETFLG
                     HRASESKTTEVKARKTKKIAKK"
     gene            22345..24870
                     /locus_tag="I9760_000913"
     CDS             22345..24870
                     /locus_tag="I9760_000913"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012301.1,HMM:NF012722.1,HMM:NF014567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT5042691.1"
                     /translation="MSTHKKNISKQDLLQILRSVPDLYLILSPKFDIIDASDAYIKAT
                     LINRKDVIGRNLFDVFPDNPGDLNATGTRNLRNSLENVLKNKVPDTMAVQKYDIRSND
                     ENVNSFEERYWSPMNIPVFDENQQIKYIIHRAEDVTDFIHLKELGTKQKKLNEVLTSR
                     AGTMEQEIYQRAQEIQETNKELRVAKELAEQANQAKSAFLATMSHEIRTPLNGVIGMT
                     SLLEGTTLSEEQKEYVKSIRISGETLLNLINDILDFSKIESGHFELDYVDFDIRQVIE
                     DTVEIVAYKAHAKNLAIGALIDKDVPSWVNTDSSRLTQILINLLSNAIKFTQQGQIEV
                     NVTRKKNKGKKSNGYITLLFEVKDTGLGITPEVMGRLFKSFSQGDPSVSRKYGGTGLG
                     LVISKRLSEFLGGEIGVDSIPGKGSRFWFTIKVKEVVANTPKVMEYFLPRLKGMHMLV
                     VDDNEINRNIFKAQLETWGISCDVVEDGFEALKKLATVKNKVNPYHMILLDYNMPGMD
                     GIELASKILEIKEFAKTPMLLATSLGLPIARERLDELNISSYISKPVRQSKLYESIIS
                     ILKNTSKTQESESIFKKDKSIITEHNERILLVEDHAINQQVAIHIFERLGYKRIDLAN
                     NGLEALEAFNTKSYDLIFMDCQMPEMDGYTATSKIRNIEQFKGLHRVPIIAMTAHALK
                     GDKEICLKAGMDDYLAKPISLPELEQVILRCLANKASKNPMGTLGGKNDNGLDIERLQ
                     LIFGGDNAAIKEFVNTFIVQAKILLNELIVALEKHDHISSKEMCHKLKGMCGNASAIQ
                     LYQLSIEQEQMILSKQWQEAIALHEKMLLSLDALSHHNVKCSE"
     gene            complement(24997..>25169)
                     /locus_tag="I9760_000914"
     CDS             complement(24997..>25169)
                     /locus_tag="I9760_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAT5042692.1"
                     /translation="YIDRKYVSFSIKDSSNSGILGTIAGDDTVLLIIKSKSDQQKVLD
                     ILMKEFPYLFAS"
BASE COUNT         7730 a         4346 c         5140 g         7953 t
ORIGIN      
        1 atttaattag gttaaaattg caccacttag gtcaattttt tttggaaatt atttagagag
       61 cccaagtcca tcagtctgtg gcttttcaaa atgtcggata aactcaagtt ttgcatctga
      121 gcaaaattag tattttcaaa aataataaat ttgtgctagt ttggaaaagt ttggaaaaga
      181 gttcttgcat tttgaaaatg gtagttggtt atatgcctag aaatattagg atactatcca
      241 aatagttgaa ttaaaaaagg aaaatacttt tttattatct taagtattgt cctgatttgg
      301 atcatcctta attttgcagt tgagcattag tctacgctaa aaaattaact gaaaaattat
      361 aggggaatat aacaaacctg ggcactccag gaagagttgc tgtatgagaa tttagtttaa
      421 ggagttagaa tcaatgcacc caaattatta tttgtcacct ttagctgtag ctatagcatt
      481 agggattgct tcacctgtga aggcagccga cccgatccct ttacaaaagt catctttttc
      541 tgaagtcaca caaaaatttc aattgacttt gcctggtgtt atgaaaggtg ctgtagtttc
      601 aacgaatagt ttgcagttta taagacagca tactgatggg aataaagtga cgcatgttcg
      661 tatgcagcaa caatatgccg ggtttccagt ttttggtggt tacgctattc tgcacagtaa
      721 gaatgccact cctagtttag cgacggcaaa atctgatgtg aaaatgaatg gtgtgattta
      781 tgatgggttg caagcagaat taggccagcc taaaccttca tttgtgaaaa atgcttcttt
      841 ggcattacaa caatttaagg ataaatacgc gaataaacaa attagtgaag atcaagtaac
      901 cccaatgatt tatattgatg aaaaacatca agctcattgg gcttacaaag tcagcgtttt
      961 agttatccat gacgatagaa ttcctgagcg accaacagca attattgatg cagaaaccaa
     1021 taagcctttt gttcaatggg atgatgtaaa aacggaaaaa gttcaagcaa aagggatggg
     1081 ctttggtgga aatcgtaaaa ttggtgaata tcaatttggc aaggacttgc cattattaga
     1141 aatcacacgt gattcaagtg ttgaaatgtg ctttatggaa aataccgatg ttaaagtagt
     1201 cgacatggga cataagtact attcaaataa caaacctatg caatttactt gtaaagagac
     1261 tcccgacact caatcgacta aaacttatta taccggatat tcggctgatg gttatgatag
     1321 agataatgga gccgcttctc ctaccaatga cgcattgtat gcaggttatg tcattaaaca
     1381 catgtaccat gattggtatg gggtggaggc attgactaaa tcagatggat cgccaatgca
     1441 attagttatg agagtgcatt atggtcaagg ctatgaaaac gcctactggg acggcaaaca
     1501 aatgaccttt ggtgatggtg acaccatgat gtatcctttg gtttctctag gtgtaggtgg
     1561 ccatgaaatc agtcacgggt tcaccgaaca acattctgga cttgaatatt tcggtcagtc
     1621 tggtggcatg aatgagtcgt tttcagatat ggctgcccaa gctgctgaat attattcggt
     1681 tggaaaaaac agctggcaaa tcggcccgga aataatgaaa gaagacagtg gttatgacgc
     1741 attgcgttac atggataaac caagccgtga tggtatgtct attgatgtag ctgatgatta
     1801 ttatggtggg ttagatgttc attactccag tggagtgtat aaccatttat tctacatatt
     1861 agccaatcaa cctaattgga atcttcgaat ggcttttgat gtcatggtga aagccaatat
     1921 ggattattgg acaccttatt caacatttga tgagggtggt tgcggtatgt tgagtgctgc
     1981 caaagacctg ggttacaatc tggatgatgt taagaagtca ttgagtgaag tgactataaa
     2041 ttatcaatct tgttatgtcg attaattcat tttattgatt gataaaattt agccagaagt
     2101 cgactgatga cttctggctt ttccatatga atatggtgtc caccatctaa cctctcaatt
     2161 ttaatatttt ttactgcttg aatccttgct ttcatcttat ccgaatcaaa tgaaaatcct
     2221 ttgctggccc aaataaggta agtttgagct tttatttctg taaggcagga aagaacttgt
     2281 gcttcagtca tttgtaatgg agatggggct aatagcctgc gatcgtgtct ccaataatat
     2341 agaccatttt cttgttgaat tccgcgttcg caaagtgact tggcaatatc cagagaaaca
     2401 tatcctttaa cggatcgggc aagagcagca tgttcaaact tattgtagcc ttttgccttt
     2461 tttgattgtt tttgtgatag atagtccagg tattttgaca gttgctggca tgctgtttct
     2521 gccgggtgag aaaaagggcc taaaccttcg attaagctta atgaaagaaa acgatccggc
     2581 gcaaccccag ctacaagact tcccaagcag gctcccatag agtgccctag caagtgaagc
     2641 ttatttagtc ccaaggcatt aataatttca acaacagtaa atattccatc agtaaaatga
     2701 tatatacatc ctgggggtaa gtgtgaagaa tgaccatgtc caggtagatc tacggctata
     2761 aaatagtaat cattctgtaa atgttcagca atattatcaa aactattagc attatctaac
     2821 caaccgtgca aggcaagaat aggcggatta tcaggatttc cccatacttt gcatgctata
     2881 gatagtcccg ggatacttaa aatctttgtt ttcatcaatt ttctttggtt tgtaggtaat
     2941 agttatagct tattacttta tacgcaggga tgtaaattca gtttttaaaa tagtcaggac
     3001 ttgcgaaaaa aataatctct tcgctaaaaa atgcttttaa tccagtcatt tagtttattt
     3061 aatcttcctc atcaaaatca atttttgcct tggtcttggt ttttttcgca agtcttgata
     3121 gtaattgaaa tagacaaaaa gcaataaagt aagtcgatta ttgggttgac ttgtaacaaa
     3181 ctataaaaga tcacaaaata aatggcttta ctggtttttg gggaagggcc aatattatga
     3241 taaataaaaa taattcagag tccgagttaa tcatgtttaa tgacaaattc ttttcaaaat
     3301 gtgggttata attatagagc aatacattaa tcctattacc attatactga ttatagaaac
     3361 ctaaatgaga gatagagtat ctatgcaacc tgaggtgcgc agcgtatcgc tgataacata
     3421 taatattcat aaaggttttg gggtaggtgc agtaaggttt ttgcttccag aaatgcgaga
     3481 tgctatcgct ggattaaatc cggactttgt ttttttacaa gaagtgcaag gtaaacatag
     3541 aaaacgcgaa aaaagaattg tcagttggcc tgattcccct caatgtgagt acattgctga
     3601 gaatatatgg ccacattacg tttatgcaaa gaatgcagta tatcaatctg gccaccacgg
     3661 aaatgctatt ttaagcaaat acccgttcga gagttttgaa agtattaatc tttcaaacat
     3721 gaacagagca tcaagaggta tacttcatac ccaattaaaa ttggataata atattattca
     3781 tcttttgtgt gttcatttag gcctattcaa ggcagagcgt actgagcaat gtaaggcatt
     3841 gatacagcgt attaaagatg ttgtgcctaa aaatgagcct ttgataatgg caggagattt
     3901 taatgattgg cgtcatgtta tttctaaaat ccttgctgaa aacttaggga ttgaagaagc
     3961 ttttgtcgcg cttgaaggac aacatgcccg ttcatttcct gcaataaaac ctgctttgtg
     4021 tgtcgacagg gtgtattttc gcgggatgaa agttcaagag gttgcttgtt tacagggcaa
     4081 accttggcgg atgttatctg atcatttacc cttgtatgca cgttttgaat tattatgaca
     4141 gggttacctc cagtctcttt gttgaaaaag atttttaatg cggtcatttg gtttattcca
     4201 actccctcat ttaaaatttt ttgccttgac catggggttt ttagcaagcc ttgtatgatt
     4261 aaaattaaag cgacagcctt cctggtcgag agaaggttct gtcgctattg caactttact
     4321 ccatgtcgtt ttggtattca cccaaacaag ccatactgtt taagcgggct ctcttaatta
     4381 aagcctcttg cgctttggca atatttgctt tgtttccaca ccattccttt aagcaatcct
     4441 cttgtaatgc tcttccaaat gaaaaactta ataaccaggg ttggtggcca atttgattga
     4501 ttgcattcaa gttagcggta gcctgttggg gtgtttggcc accggagagg aaattaatgg
     4561 tgggtactga cgctggaaca ttattacgaa atacactaat ggtgtagtca gccacttctt
     4621 caggatcgct aaacggggcc acttctttac cacaggtaac catactcggt ttaagtataa
     4681 tatgctccaa ctccacttga tggataaaca aggtatgaaa cagctcatgg agaaccattt
     4741 cacagacttc tgcacaatgt tctatgctat ggttgccatc cattaatact tcaggctcaa
     4801 caattgggac gatgccgact gattgacaag cagcagcgta acggcccaaa ttttcagctc
     4861 ctgttttgat tgcagttagg ctgggagtgt attcggaaat tgaataaacg tttctccatt
     4921 ttgcgaattt tgcacctaat tttttaaaat gaagtagacg ctcagttaaa ccatctaatc
     4981 cctgggtaac tttttcatgt tctgtattgg ctaaatcaac aagccctttg tctactttaa
     5041 ttccaggaat aatgccttta ttagcaaaaa gctcaggaat tggtgttcca tgctcatcag
     5101 tttgagtaaa agtttcctca aataaaatga cgccgttgat atactgttct aaatcaggag
     5161 tggtcgctag taacaagcga taatctcttc gattttcctc ggtattttct gtattgattg
     5221 acgcaaagcg tttgccaatt gtactgttgc tttcatctgc agctaaaata ccttttcctt
     5281 catgcagcat ttgctccatg gtatttgata attcttcata actcatagcg tctttatctc
     5341 ctcattatcg ggtgtcgacc tttcgaagtg gtttggcacc tattaaattt aaataacgat
     5401 tagtcatctt ttgtcaaatc cgggttattt accgaaagga tgacattgat taacaatggt
     5461 ttttgatgcg gtatttcagc gtgaaatata tttttcacgt acaatatgtt gcatggaaaa
     5521 accatgttct tttaaataat taaatgcctc gtcgatcata cctggatttc cacataaata
     5581 aaccatatcg cgagttggat ttaaattaag ctctggaaat gcatgttgta cataaccact
     5641 gtattcattg tctttcaagt cttgtttttg cacacggctt aaatagggac gaaaacttgc
     5701 ttgaggatat ttttttgcaa aggcttgaaa atcatctgga tagagaatct cttcgctgcg
     5761 ttgcactcct tgaaggatga ctacctgcaa atcaggatgt ttttctattc tttgaccaag
     5821 ctcattaagc atggctctat aaggtgtggt accagtacta gtcgctacca atatgtatct
     5881 tcctggagtt tcatctctaa aaatcaaacg accgaaaggt ccattaatgt ggatcacatc
     5941 acctggcttt aaattgtaga gtagttctgt gcctggtcct ccttcgaaat acccggcagc
     6001 aaattcaatg cgattatcct ttttgggttc attggcaata ctgtaactac gctttaagtt
     6061 ttttccatca tgttcaaaat gaatagtgat aaattgtcct ggtaaatatt caaaaggggg
     6121 tgataattca caattgaata taaagtgctt taccttggga gaaatcatga aagactcttc
     6181 tagagtgatg gggaaagtat ttatttgcat aaggaatatc aacttttatt ctaaaatgat
     6241 taatataggg gaaaaattat gcattgcaag agctaaacgc atataattaa catatgaaca
     6301 cgatagattt aatcactata ttaggtaata ttagccaatc cctttatcct gtgcaaaaac
     6361 ttatcacagg aggagcttat atattgggtg tcttattttt tttaacggca attggcaagt
     6421 taaaaaaaat cggggatcat cgggctcaat cctcctcaca ggaaaaaatg tttactccaa
     6481 tgatgtattt attagttggg gcacttttaa tctacttgcc ttcgggtatg gatcttatgg
     6541 caaacaccgc ttttggtgtc ggtaatgtat taacttattc caattataat cctgccaata
     6601 tctacagttc aatggggttt ttaattcgca cggcgggtgt tctgtggttt atacgtggat
     6661 gtgtacttgt cgctcattcc agccagccag gtactcaaca aggattaaaa ggattggtat
     6721 ttcttattgc aggcgttttt gcaatgaatt ttgataatac gattgccatg attaattctg
     6781 ctttagatta ttttgtcaaa gcaactttat cagtaaaagc cgcagcggga ttctagtttt
     6841 tctgattgat ttgtctgcgc tttaaagaaa caggacttag gaaaaacccc aagatcgagg
     6901 caaaaatgtt tttaaccaag aaactggggg ttttcctaag tcctgagaaa ataaaactgg
     6961 agggcaatcc tttggaatgt tataagtctt cttcaccaca gattttcaag ttgttagctt
     7021 tagcaaaatc cataattgcc tgataaactt gtgggcttga ttccttgagt ttgggatcaa
     7081 gtaaaacaca tttataaccc tcactattaa cgcttggttg caagagcgga gaataataaa
     7141 ttctactatt tttaaaaaca gctaaagaac cgctcatacg ctcggcccag tcacttgggc
     7201 gaaatgtttt gccttgtgaa gtaacaccct caatgacaat agttttattt tttcgattag
     7261 acataactct atgcatatta aatacagtat gataattata tcatcatcat gctgggcctg
     7321 cagggaaaat taattatttt attgcaataa atggttgttt cgctttagtc attgccaatt
     7381 attagtcaca attgcatgct gtctttatga ctaagcataa gtttccatag tagatttagt
     7441 tctacttatg atagcatgtc cttgatttaa aaatgaatga ggtatgattg cgaatgaata
     7501 aagaaaatca ttctgggatt tatttgttac caaatttatt aacgactgtc agtttatttg
     7561 cggcatttta ttcaatagtg gcttccacaa agggacaata tgaggctgct attgtcgcta
     7621 tgtttatagg aatgatcgct gacagtcttg atggacggat cgctcggtta accaatacac
     7681 aaactgagtt tggtgctcaa tacgatagtt tgtctgatat ggttactttt ggagtagcgc
     7741 catccttgct gttatataat ttaactctgc atcaattagg aaaatttggt tggttagtcg
     7801 catttattta tacagcggct gttgcgttgc gtctcgctcg gtttaatact cagattggaa
     7861 aatcagataa acgttatttc cagggcctcc catgtccacc ggctgcagca gtaatggctt
     7921 cttttgcttg gatctgtttt caaaatgaat gggcaaattt gattgtatct ttatttacag
     7981 ctgtcctggc tttgattgtt tccgtattga tggtgagtaa tttacgctat tacagtttta
     8041 aggaagtaga ttataagggc aaggttccat ttttatatgt tttagtgatg attcttttgt
     8101 ttgttgcaat tgctgcaaat cctgcaatag tgctttttgc aagttcaggt atttatgctt
     8161 tatcaggccc tatgttgacg gtgatttcat tacataaaat gaggaaacaa agaagaaata
     8221 tgagcaaatg acatggattg tgtatttctt gatgaattat tatgtaaatc tgttacccag
     8281 acctgtgaac agtagatttc ttcagattag tatcaattaa ggtgtattga gaccttaggt
     8341 cagtttattg aatcacaggt tgatcataat aaagatgtta caagaactgc gaggaacatt
     8401 gatggagaac aggaacctct gtgtgtcgca aaaagatatg gagcagaggt aaacagcgta
     8461 gtttagtatt ctttattcac tttccccaaa tcgattattg attaattcga ctaaagcttc
     8521 attcatgcgt tcttcatctg gaccgtcaat ttccaacgtc aactcactac ctttgttagc
     8581 agcaagcatc atcacaccca taatgctttt gccattaacg gtctgagtat ctttggttac
     8641 atcaatatgg ctttgaaatt ttgctgctgt tgagacaaat ttcgctgagg cacgagcatg
     8701 taaaccgagt ctgttgatta tctttatttt ggtcttgatc atagcacgta caatgtatca
     8761 tgactctggc atcaggtaca gtgattaaat aagtgatttt agtaatcaac cctctttctc
     8821 agtgctatga cttcatctaa aaaacggtta tttgttttat gtcagtttac ataagcactg
     8881 agaaggtgtt gatattaaat taaagtaaaa gggtttgtat ttaaaataac atttaatccc
     8941 tgtctctatg ggctaaaagt ttttctttat gtttaatcag ttgtcgattt aatttgtcta
     9001 ccaaagtatc aatagcagca tacatgtctt ctgattctga actggcatgc aactcacctt
     9061 tggtaaccaa tacggtcgct tctgctattt gtcttacttt ttcgacatca aatactacat
     9121 taattgctct gatgtgatca aaatgacgct caagcttatc aaatttttct tcagtgaatg
     9181 ctcttagtgc aggagtgaca tccataccat gtccagtaat gttgatttgc ataaaacctc
     9241 ctttcgttga ccgattaatt aactactaat aacctttctt tcattggatg ggggaatccc
     9301 cattgcttcg cgatatttgg caacagtgcg tcttgctact ttaatgcctt gctcacctat
     9361 tagttgggca attttactat cactcaaagg tttttttcta ttctctgctg aaattaattt
     9421 ttttattacc gctcggattg cggtggaaga gcattctcct ccgctatcag tattgacatg
     9481 gctggagaag aagtacttta actcaaaaac acctcgaggg gtgttaataa atttttgagt
     9541 tgtaactctt gataccgttg attcatgcat atccaaggca gaagctacat cattcaatat
     9601 taatggtttc atcgcttctt caccaaattc cagaaatcca tgttggtatt ccatgataca
     9661 ggttgctact tttagaagtg tttcctgacg gctttgaatg ctttttaaaa accaacgtgc
     9721 ttcttgtaaa ttatgcttta aaaattgatt atccagacta ttgtttgcgc gttggattaa
     9781 agcggcatat tggctattga tactcagcct gggaagaata tcttgattca aaaaaacttt
     9841 ccattgatta tctatctttt taacagttaa gtcgggaata acatattcaa tattatcttg
     9901 atggatcaaa cttcctggtt ttggatttaa tttatgaatg acttgtaata tttcatgaat
     9961 tgttttttca cttatttgat gatttttcat gagttgtctg tagttatgtt gccctagcaa
    10021 ctcaatgtct tcaagaataa ctttcttggc agtagccaaa agcttgggtt cgagtggaag
    10081 ttgttccaat tgcaaaacga gtgtttctgc taaattaaca gatgcgcatc ctacggggtc
    10141 aaataattga agcctgtgcc tcactgcttc tatttcttct atatccagag gaaaatcatc
    10201 gctatttaag ctggcgtgaa tttcagcaac gggtatggtt aaaaagccgt catcgtttat
    10261 cgcatcaata attgttgtag caatgactcg gtcaatatcg ctcataggcg tcaggtcaag
    10321 ttgccagtta agatgatctt gtaaattggt ggtagtgcag tgtaaattat cgaaattgta
    10381 atcgttttct tcaaaattat tgcgcttgct taagttatta tacagctgtg accattgaaa
    10441 atctgaatat tcatcttggt tctgattggt atttatttgg gcgtcttctt tttcttcaac
    10501 aggagtggct tcaagcaaag gatttgattc aacaacgagt tgaatttctt gttgtaaatc
    10561 aaaggtggat aattgcagca gtcttattgc ttgttgcagt tgtggggtta acgtgagatg
    10621 ttgaccaata ccaatctgca atgtcggttt cattcaaatc ctcttttttt ttcactgtct
    10681 ttaactatca gtttaaacga tccacgagga ttatccagtg cttacgggcg ttaatttgaa
    10741 tttaaaagta atggaattac ctatattttt aataaaatga taggcttgtt ttgaaatata
    10801 acaaattggt ttttaaagtg gataatgaga ggaggtaagc cccaaagaca ggggcctatc
    10861 aatttatttt acttttttct ctttgaacag tacgtgcttt cgtactttag ggtcatacat
    10921 cattaattcc atcttttcag gatggttacg tggattttta gtagtggttt tatagtatcc
    10981 agttcctgct gtggattcca ttttcacttt aattgtaaca gctgccatgg cttatcccct
    11041 tgttatatct tctcgccttt agctctgatt ttatccagaa ctgcctttat gcctaacttg
    11101 tcaataattc tcataccctt ggtacttaat cgcaaggtga caaatcgatt ttcttcttca
    11161 acccagaatt tatgattctg aatgttaggc aggaagcgtc ttttggtttt gttattagcg
    11221 tgcgacactt tatgaccaac catgggtcgc ttgccagtta cctgacatac tctagacatt
    11281 gtgttactcc aagataaaaa aaattttaac agcccttacc aatgcactac agtggtgttt
    11341 attttggcgg gctttcgttg taaaaaataa ccagatatga aatacaagag cggttttata
    11401 acaaaaaaac ccaaatattg caatattttt actttgtttt cattaaaaac tgatttttca
    11461 gggctgttgt ccaaacaggt atctggtaaa agtagaatat ttaatcaaaa tcagtataat
    11521 cctgaaattt tatattgagt tattgtaatg caacgtaaga ttaaaagtta cgtgttgcgc
    11581 gctgggcgaa tcagtaacag acagcaacaa ggattagatc tttggcttga agactatgaa
    11641 ttaaaatttg atagtccctc tccctggaat tttgcaaagg aatttggccg tcatgatgca
    11701 gatactattg tagagattgg ttttggtatg ggtacctcgt tgtttactat ggcaatgaat
    11761 aaccctcaat gtaattatct gggcatagaa gtacataaag caggtgttgg tagtctggta
    11821 gcggatttgc atgagcacca gataactaat gtgcgggtag tagtgcatga tgcagtagat
    11881 gtattacaaa ccaaaatacc agaaaactca cttgccgggg ttcaaatttt ctttcccgat
    11941 ccgtggcata aaaagcgtca tcataagcga cgactgattc aatccgaatt tattcaaatg
    12001 ttagtaaaaa aaatcaggcc atcgggattt attcactgtg ctactgattg ggaagattat
    12061 gcagagcaca ttttaaatgt tttatcttca gaatccgcct tgtttaacca acaaaaagag
    12121 ggcggatact caccacgccc ggactcaagg ccgttaacaa aatttgagca gcgtggtgag
    12181 cgtctaggcc atggggtatg ggatctggtt tttataaaaa aataaggaag ttacctatga
    12241 taaagcatgc aattcttatt agcggattga ttgtttcctt tttagcttca gcagctgaaa
    12301 tgtcgtttct agcccaattg acggaagtac ctggtgcctc tgggtacgaa aaaaatgttc
    12361 gtcatttggt acaaaaacag tggcaatcct acatggcgga gctaaagatc gatggtatgg
    12421 gtaatttgat cggccagcgt aaagataatc agaaaggacc gaaattacta ttcatggcgc
    12481 atcttgatga aacggggttt atggtggaat ccataacacc tgatggcttt ttgaaagtta
    12541 tccctttagg cgggatcgca aactcggtca tctatgctca acgctggaca gtttcaacac
    12601 ccaaaggacc tgtattagct tattctggca tggattctcc gcatttattg ggtgataaga
    12661 aaatgtcagg gtctcctgat atcaatgccc tgtttttaga tatcggagca gagaataaag
    12721 aacaagctca gaaagaattt gcaatccaac ctggccttga agtcacacca gtaagtgaat
    12781 ttagccaatt aagcaagaac agatttttag gcaaggcctt ggatgatcgc ctaggattag
    12841 ctgtcattag tgacgctctg agagcagtga aacaacaacc caatcaatta tatcttgctg
    12901 ccacagtgca agaggaagtt ggtatgcggg gtgccagcac agtatacaaa gcgactcatc
    12961 cagatattgc aattaatgtt gaagtgggaa tcgcagatga ttatccaatg ctgctggctg
    13021 atagaaaagg tcgaattggt ttggggaagg gaccatcctt gtttgtttat gacaaatcaa
    13081 tgattcccaa tcaggagtta attaactgga taatggaact tgctaataaa aataatatcc
    13141 cgctacaact tgaggtggag ccaggatatg gtgaagatgg ctccaaggtt caggcatcag
    13201 gcatgggcgt tcctgtcgtc aacattggta tccctattcg atatgcacac caacatgccg
    13261 ggatatttga taaaagagat tacgataata cagttaaatt gttgaggttg attgctgaga
    13321 atttgaatca ggaagtggtt gacaagatta aaaagagtta acaatgtttt gatctcccat
    13381 tgtccattgt gtaatattgg tgtcctataa atagtttata aataaaggaa gtattattat
    13441 ggattttgta agtgaaatga ataagattct acaaatggaa ttagagcaat ttaaagaatt
    13501 tattcaaaag aagctggaag aagataaaaa ttatttagaa aagttatttt ggttgccgaa
    13561 tggttctcaa atgactgttt taaattattt gatcgaacaa tactctgagc ctaagcttgg
    13621 tatagacgat gcaaataggg atttgcttgc caaaatagat tttgtccttc acgaggctaa
    13681 agatgtgaat gttggtgagc ctttgcacca ggcaattgtt gctggaaaaa tttccttggc
    13741 actccatctg ctgggagtgg atgaaaataa tttcacaccc ggggagagtg aaaaaacgga
    13801 tgttttgagt attttgtcta aagtaaagaa aaaaatcgaa gagtctttta atcacgtaaa
    13861 aagatgtttt tttgatgtgg ataaacggga tggttatggg cgcactttgt tgtctttggc
    13921 attagatgcc aaacgccagg aactgttaat tgccatttta gcaaggaatc ccattgtaca
    13981 cgcaacaacg ttaagatcct cagcctatgt tcctttccag cctattcatc aggctgttgt
    14041 tttagattat gcagagggca ttaccttatt agccagcatg ggggcgcaat tgacaaatcc
    14101 cctgggtagt atgagagaca ctccggtcat tttggctgct cgtttgggca aaataaatgc
    14161 actggcggct ttactggagt tacccactca aagtttgtct cttgaatccg aaaacaatca
    14221 tttattcgaa gacaaacaaa ctggccatac agcagtggaa gagttatgtg aacgcatggc
    14281 caatgagaat gataaagcag aggctcttcg tgggattgcc atgctaatat gccgtggtgc
    14341 tgaaccacct cgcaatgaaa aaatgcgaaa tttattaagc agcaaccggg ttgctttctt
    14401 aaaagcggtc agtacctatt tggcagataa gcctcaattg gtcgatgctt ttgtagagcg
    14461 ttgtcatcta cgagaaagtg ctttgcataa tatagtttat gccgatcatt catgggggag
    14521 ttctattcgt catttactcg gtgttcccag tgaggcggcc cttattgttg aagaattagt
    14581 aacgagaaaa tacagtgatc cttcctttgc cagcgaaaat gtttcgtcat tatcaactac
    14641 tgctacggaa aatctacgtt cagagaggaa cccactgaaa ctctatgccg aatttgtcag
    14701 gcgatacacg caagcatatg atagccaaat gattaccaat cgttggagta ccatgcgctg
    14761 gatgatcgcc gaagggaatt gtgattggga tacagtagtt cggtattcga aaaatcatcc
    14821 aacgagccgt accaggattg tgttgaatga aatgtttaac ccgataccga gggtacatga
    14881 ggatattgag tctcaacaga attctccaag agttgttcta aaataattta tgtcaatatt
    14941 ttgatcttga accttcgaca gacaccttgg cggtctgcga aggtttataa aacatcttga
    15001 gtatttccag atgattgcca agtacagata gtcttttgct tcatttcaat atacaagtcc
    15061 tgaagtatct acttgcttaa acttcatata ccccataaaa tcctaaattc atttgaaatt
    15121 gctaggagta gtacatgggg tggaatgagc cagagaaggg caaagatcca tggtcgggta
    15181 aaaatcaacc tccggatttg gatgaagctc ttaaacgtat ccaggataaa ttgaagaaaa
    15241 cattttttgg aggcacgggc aaatcgaata ataattcgtc aggtggctcc agtggcggat
    15301 tgcttgctgt cacagtttta ttaatagctt ttattctttg ggctctttct ggtattttta
    15361 ttgtggatcc tgcagagcaa gctgtgatac tccgctttgg taaatatgca gaaacagttg
    15421 gtcctggtcc tcattggatt cctcgtttta tctcatcaaa gattgttatg aatgtggata
    15481 gggtgctaga ttactcctac tccgctcaaa tgcttaccag tgatgaaaat ttagtatcgg
    15541 tctctttggc tgtccagtac cgcattaatg atttgagtga gtatttgttt aatgtcgcaa
    15601 atcctgagga aagcttacaa caggcaactt ccagtgcttt aaggcaggtt gtaggcacga
    15661 caaccctgga tcagataata actgaaggtc gagaagtttg gggaggtaga gtacaagaaa
    15721 ctctgacgaa aactttagaa agctataaga ccggaatttt aatcgttaat gtgtcacccc
    15781 aacctgccag agctccagaa agtgttcagg atgcttttga tgacgcgatt aaagctcaag
    15841 aagatgaaaa acgtttcaag gaacaggctt atgcttatgc agcaaaagtc gtgcccattg
    15901 cagaaggtaa agcaagtcgt attcaacagg aagcggaagc ttattcaaag caagtcgttt
    15961 taagggcaca gggagaagta gcggaatttt tagccttact tcctcaatac aatgcagctc
    16021 ctcaagttac ggctaaacgg atgtaccttg aagccatgca aaaagtaatg aataagagca
    16081 gtacgattat tgttgacagt aaagcaggaa atttattgta tctaccttta gataaactac
    16141 ttggtaatca atacgttccg caatctggaa atctaaaagc cataaaaaac ggtacgaatg
    16201 tcagtaacga ggaggacaac ttgattcttt ctggaagaga tctcacaaga ccaacttata
    16261 gtcaagggag agattgagaa tgaatgcatt taaaacgaca ttgggtgtct taatcttttt
    16321 ggtactagtg cttttgttta ccactatgtt tactgtcaca caagggcagc agggtattat
    16381 tttacgttta ggacgtttgg taaaagaccc tcaaactgac gcagtgaaag tgttaaatcc
    16441 tgggctccac tttaaaaccc cttttatcga aagtgtgcga attttcgata cacgcatcca
    16501 aaccatggat atcaaatcga cccgtattgt gaccaaagag aaaaaagatg tcatggttga
    16561 ctattatgtg aaatggcgaa tttcagattt ggcccagtat tttaaatcaa caggtggaaa
    16621 tgaatttaaa gctgaaactt tgctagaaca acagttaaat accttgctca gagctcaatt
    16681 tggtaagcgt accatatccg atgccgtttc aggagggcgt gatgatgtta tggaaatact
    16741 gcgtaatgct gctgagaagc aagctggtga attaggtatt aaagtggttg atgttcgtat
    16801 taaaggtatt gaattgcctt caaataccag taatgccatc tatcaacgca tgagagctga
    16861 catgcaaaaa attgcaaaca gacatagagc tgatggacaa gctgccgcag aacagatcca
    16921 ggcaaaagct gatgctgatg tcacggtttt attggctaaa actaatagta atgctcagcg
    16981 aattagagca gttggtgaag cggaagcagc ggctatctat tccaaagcat acactcaaaa
    17041 tcctgatttt tttgcattat acaaaagctt gttagcctat gaagccagtt ttcatagtaa
    17101 aaaagatatt ttaatattgg atcaaagcag ttcatttttt gattatttca aacaagctat
    17161 gccaaaaaat gatggtacag cggctaaaaa gtaattataa tgtgaaattt tgctgtaatc
    17221 aggttcgata tttattagca tcatccatct aatataaatg cagttgggtt tctcgtaaaa
    17281 tacaactgca tttttaattt ttacaggtat gcaaacaaag agtaaatcat gggtaagaat
    17341 gttgttgttt tgggtacaca atggggagat gaaggcaaag gcaaaattgt tgatttgctg
    17401 acacaagatg ctcaagtggt tgtccgttat caagggggcc ataatgcagg ccatacatta
    17461 aaaatcaatg gagtaaaaac agtacttcgt ttaattccat ccgggatgct tcgccctaac
    17521 gtaacctgct atattgcaaa tggtgtggtc ttatcacctc aggcattact tagtgaaatt
    17581 aaggagcttg agggtaatgg cgttaatgtg agggagcgat tacgcattag cttggcttgt
    17641 cccctgatcc tcccttacca cattgctttg gataaggctc gtgagacaca tatgggcaag
    17701 tctgcaattg gaacaacagg tcgtggtata ggtcctgctt atgaggataa agtggctcgg
    17761 cgcgctttaa gagtaggtga tttatttcat agagaccgat ttgccaataa attaacagaa
    17821 ttattggatt atcataattt tgtattaaca caatatttca aacaaccagc tgttgatttg
    17881 gaatccctat tggatgagtc attacaatgg gctgaagaat taagaccaat ggtttgcgat
    17941 gtttctgcct gtttgcatga acatagaaaa caaggtgaaa acattttatt cgaaggggcg
    18001 cagggtgttt atttagatat tgatcatgga acatacccct acgttacttc ttcaaatacc
    18061 tgtgtgggat ctgtaattaa tggtgcggga tttggtcctc gctatattga ttacgtgtta
    18121 ggtatcacga aagcttacac aactcgtgtt ggtggtggcc cattccccac tgagttactg
    18181 gatgacgttg gaaaacgcat tgcagaacga ggtcaggaat tcggtgcagt aactggtagg
    18241 cccagacgtt gtgggtggtt tgacgctgtt ttattaaagc gctcgattga gttaaatagc
    18301 atttctggac tctgcgttac caaattggat gtgcttgatg gtttagaggt attgcggatt
    18361 gctgtggctt ataaagacag ggatggcaat atattatctc gacctcctct ggcagctgat
    18421 gatttcaacg atttgttacc agtctatgag gaacttcctg gttggcagga atctactgct
    18481 gatgtgactg tgatgagtga cttacccgcc aatgcaaggg cttatctaaa gcgaatagaa
    18541 gagatattag ggattcctat tgatatgctg tcaacaggac ctgaaaggga ttcaacaatt
    18601 actttacgag atccttttct ttgaatgtaa ggacagagtt ttgtaatcac tgtgggtgtc
    18661 atgcaaggat ttcttgatgt gacactttcc gccttttaag tattattgtc tgtgttttag
    18721 caaactattt ccttggggca gcaggcaatt cttgcatcgc taaatttcat ttcatgctgg
    18781 ttctatgaac gaaatttcta acgcatccat atgagcgctc cggccaaatg caaaaatcca
    18841 gagaaagcgg aagttgtttt atcaaaacga gaaaaaattc tgcgaaaatg ctttaatttg
    18901 aaaaagaagc ctctatcaga aagcgctcct tataaagttc tttatctgtt tctctggggc
    18961 gcttatcgct ttttcttgat ggaatgacgg aagagtagcc ttggttatgt aacgaatcaa
    19021 tgagttcttg tgaatcatat cccttatcag caagaactat tgtgtcgtat tgttccttga
    19081 gcagctctgc tgcttgagta atatcattcc ggtgtcctcc ggtcagaata aatttaatcg
    19141 gatttcccag tccatccacc agcgcatgag ttttagtggt aaaaccacct ttactacgcc
    19201 ctaatgtctg ggcttcattc ccattcttga cataacccga tgcgcaagcg tgagcacgta
    19261 caatggttgc atctatcatc atagtttgag catcaatatc acttacaaaa gacattaacc
    19321 cagactagat accccgatca gatcacgatt tataacgtcg atgaatctga ggtcaatgac
    19381 cataatagta agggagcaat cgcattggca gccagtacgt aatacatacc aacctccttc
    19441 tataaaacgg cgtaacgcct attgattctt gacatgtaga cctttcaagc tgcgcatgaa
    19501 agaataaatt ttatcccata gtttttctga tatgttatga tacatttagg aagttccata
    19561 atgtgttttt tacgatcgcc aatcgaattg cactataaaa ctgccttctt ttcaattatt
    19621 taaaatttcg ttcacagaac ctagacaaag gatcgagttt aaaaattatt ttgttttcat
    19681 ccgaaccgag cacaggtgga tgactaaatc gtttggtttc ataatctggt aatacttcaa
    19741 ttaaacgcat tagtccattt ctccgattcg ttctaccgcc tcgctcaaat cgaaaaaagt
    19801 ggattggcaa tataattttg ttgtatccaa tcgctcttgg cctgcaagag aagccatttt
    19861 ttcgaggcta atgtcggcat ttaccaaatt tttacaaaat gtatacctta gttgatgggg
    19921 ggaaatcatt cctaactctt ctggggtgcg taatcgcttc agcatgagct gagcccctcg
    19981 ttgaccatta aaaactaatg catcgcttcc tgcatgtaaa gcataatcta aagtaagaaa
    20041 cgcaaatcgt gcttctttat ttacatcgtt tccctgcatt gattgtcagg tgtcctttac
    20101 gttcatttaa aacaatatga ctccatttta agttgcatag ctcacttgca cgaagccccg
    20161 tatttaataa gatttttaca atcgcaatat ccctcctgcg gccattttgc tcaaaatggt
    20221 tcagtagctg attttgttga ttgcgattca actattggtc ttcaaaaatc aatgacattg
    20281 gtggtccgac aaatctaaaa ccctgaccga tctaaacaag ctatctaaat gttggtgtgc
    20341 ttacttacaa aacggctata cttaaaatca catatgggca aaagttgatc taattgacta
    20401 gtaaaggaaa tatttttgtg ataaataatg cgttgatatg cacaaaaaat aatggcttag
    20461 ctaccctagt ggcatctgta tgccaatatt gtggttttaa ggatttgtgc gtaaaaaacg
    20521 ctagagaaat ttcaaatatc cttgaaaatg aatctattaa gttaattatt cttgatacgg
    20581 cgttacaaga gccttgtatc atcgatgtat tatatatttt atccaattta aagtgcgaaa
    20641 tacctatcat tgctcttggt gagtgtgacg ataaaattct tttatccata aaaagaattg
    20701 gtgaattaaa aaagttatat gtttacacca tggagttgaa ttctttttca gaagaagcat
    20761 tctgtgaaat cttgaacctc attgataaaa agagccaaat cattaatgaa gaggcaatta
    20821 cgatagcttt agagaaaaaa caatttttca tgcattatca acctaaaatt tcattaagta
    20881 cttataaatt ggttggtgta gaagccttaa ttcgatggca aaggccaaat catggtctcg
    20941 ttcaacctga ttcatttatc cctgtcgcag aaaaaaccgg cttgataatt cccatgacct
    21001 attgggttat tcaggatgtc tttcgacaat actcagcatg gaagagaaag ggtattcagt
    21061 taaaatttgc cattaattta tcaccaaata tcttaacaaa tgtaatctta cccgacgaac
    21121 ttggtaaatt ggtaaaagaa tacaagatct gtccagaaga tatttgcttt gaaattactg
    21181 aagctgccgc tatgcattgg cctgatatcg tattagaagt attgactcgt atccgcttaa
    21241 aaggtttttc tctttcaata gatgattttg gaacaggtta ttcatcttta gttgagttgc
    21301 aacgattgcc tttcacagag ctaaaaattg ataaatcatt tgttagtgat ttagcctaca
    21361 ataaatctaa tatgcatatt gttaaatcaa tcataggatt ggggaaaaat atgaatctaa
    21421 ccttgatagc agaaggtgtt gaaacaaagg aagttgctga taaattatta gccatgggtt
    21481 gtgaaaatat gcagggatat cttatttcca gacctttatc agaaagcgaa ttcttgaatt
    21541 ggtatagaaa caagattgat aaaaatgaga ttttcacagc gtaaggatgc gcttatgaga
    21601 acaattaaag tccaaggatc aaagcccacc attttaatcg ctgatgatga tttatcaacg
    21661 cgaatgctgc tgagagctac catttctcaa tgggattatc ccgttattga ggcaagtgac
    21721 ggtgaagagg cttgggaaat tctgaaacag aatgatggtc cccaaattgc tacagtagat
    21781 tggatgatgc ccaagctcga cggattggat ttatgtcgtc gcacaaaaaa tttacccaga
    21841 ccaccttata tgatcctgct gactagtatg tcaggagctt cgaacgtggt gaacgcatta
    21901 gactctggcg cagacgattt tttaacaaag ccgtttaatt ttatcgagtt acgcagtcga
    21961 ttacatgtag ggcaacgaat cattaattat gccaatcgac tggagtcagt cattaatgaa
    22021 aaaaaaccgc acgaaaatat ctctcaacat ttgcccatta ctatcaaaag attaaatgct
    22081 atgggcaaaa aaattttaga tgaatggatt aatcttcaaa gcatgctgca tttgttggaa
    22141 gctatggaaa aagataatca tattaaattt aaaaatcaga tacttggtat ttatgagcgt
    22201 caaaaagagt tgattgaaga aattacgagc ttggaaacat tcttgggaca cagagcttcc
    22261 gagagcaaaa caacagaagt gaaagcaaga aagacgaaaa aaatagctaa aaagtgacat
    22321 taatttattc gaacggattg caccatgtcg acacataaaa agaatataag caaacaagat
    22381 ttgcttcaaa ttctaagatc agtaccagat ctatatctca tattgagtcc aaaatttgac
    22441 atcatagatg cgagtgatgc ttacatcaaa gccaccttga tcaatagaaa agatgtgatt
    22501 ggaagaaatc tgttcgatgt attccctgat aatccagggg atctgaatgc gactggtacg
    22561 cggaatctgc gcaattcatt agaaaatgtt ttaaaaaaca aagttcctga tacgatggca
    22621 gtgcaaaaat atgatattcg cagtaatgat gaaaatgtga atagctttga ggagcgttat
    22681 tggagcccta tgaatatacc agtctttgat gagaatcagc agataaagta catcattcat
    22741 cgtgctgaag acgttactga ctttattcat cttaaagaac tggggacgaa acagaaaaag
    22801 cttaatgagg tattgacatc tcgtgctgga acgatggagc aagagattta tcaaagagca
    22861 caggaaattc aagaaactaa taaagaatta cgtgtggcaa aggaattggc agaacaggct
    22921 aatcaagcaa aaagtgcttt tttagcaacc atgagtcatg aaattcgaac tccactaaat
    22981 ggtgttatag ggatgactag cttgcttgaa ggtaccactt tatctgaaga gcaaaaagag
    23041 tatgtaaaat ccattcgaat ttcaggagag accttgttaa atctcattaa tgatattctt
    23101 gatttttcta aaattgaatc aggtcatttt gaactcgatt atgtggattt tgatatcaga
    23161 caagtgatcg aggatacagt agagattgtt gcatataagg ctcatgcgaa gaatttagct
    23221 atcggtgctt taattgataa agatgttcct tcttgggtta atacagattc ctctcgattg
    23281 acgcagatat taatcaattt attaagtaat gctatcaagt ttactcagca aggacaaatt
    23341 gaggtaaacg ttactagaaa aaaaaataaa ggtaaaaagt ctaatgggta tataacatta
    23401 ttattcgaag taaaagacac gggtcttggg attactcctg aagtgatggg acggctcttt
    23461 aaatcatttt ctcaaggcga tccatcggtt tcacgtaaat atggtgggac tggcttaggt
    23521 ttggtcatat caaagcgttt atctgaattt ctaggcggtg agattggtgt agatagtatc
    23581 cccgggaaag ggagtcgttt ttggtttacc ataaaagtta aggaagtggt tgcgaacacc
    23641 cccaaagtca tggaatattt tctcccacga cttaaaggaa tgcatatgct ggttgtcgat
    23701 gataatgaaa tcaatcgtaa tatatttaaa gcgcaattgg aaacctgggg aataagttgt
    23761 gatgttgtag aagatggttt tgaggcacta aaaaaactag ccactgttaa aaataaggtg
    23821 aacccttatc acatgatatt actggactat aatatgcctg gcatggacgg tatagaattg
    23881 gcttcaaaaa ttttagaaat aaaggagttt gcgaaaacac ctatgctgtt agcaacttct
    23941 ttaggtttgc caattgctag agagcgttta gatgagctga atattagctc atatatatct
    24001 aaaccagttc ggcaatccaa attgtatgag tcaatcatat ctatattgaa gaatacatct
    24061 aaaacgcaag aatctgaatc gatttttaag aaggataaat caatcattac cgagcacaac
    24121 gaacggattt tattagttga agatcatgca ataaaccaac aagttgcaat tcatatcttt
    24181 gagcgactgg gatacaagag gattgacctt gccaataatg gtttagaagc tttagaagca
    24241 ttcaatacga aatcttatga tttaattttt atggattgcc aaatgccaga gatggatggc
    24301 tatacagcaa cgagtaaaat tcgaaatatc gaacaattca aaggactgca tcgtgtacca
    24361 ataattgcaa tgacagcaca tgcactaaaa ggagataaag aaatctgctt aaaagcagga
    24421 atggatgatt atcttgccaa gcctataagt ctccccgaat tagagcaagt tattctacgg
    24481 tgtctggcca ataaggcatc aaaaaatcca atgggtacat taggtggcaa aaatgataat
    24541 ggattagata tcgaacgtct gcaacttatt tttggaggtg ataacgcagc gatcaaagaa
    24601 tttgttaata cctttatagt acaagcaaaa atacttttaa atgaattaat agttgcatta
    24661 gaaaaacatg atcatatatc ttccaaagaa atgtgtcata agttaaaagg tatgtgtgga
    24721 aatgccagtg ctattcagtt gtatcagttg tctatagaac aagagcagat gatattaagc
    24781 aaacagtggc aagaggcaat cgctttacat gaaaaaatgc ttctaagctt agatgcgcta
    24841 agtcaccata atgtgaagtg tagtgaatga ttatgaattc gatattctgt attatcgagt
    24901 tgatgttggt tctacatgaa gatcctggtt cgcatttgcc ccgtttctgt aatttaacct
    24961 ggctttcctc agcatttgtc aaattttaaa ttatagctac gaagcaaata aatatggaaa
    25021 ttccttcatt aaaatatcaa gaactttttg ttgatcagat ttacttttaa taatgagcaa
    25081 gaccgtgtcg tctccggcaa tagttcctaa aattcctgaa ttcgaagagt cttttatgct
    25141 gaaggaaacg tatttacggt ctatataat
//
LOCUS       DACTJE010000029        24677 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.029, whole genome shotgun sequence.
ACCESSION   DACTJE010000029 DACTJE010000000
VERSION     DACTJE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24677
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.029"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            234..2924
                     /locus_tag="I9760_000915"
     CDS             234..2924
                     /locus_tag="I9760_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0365 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042693.1"
                     /translation="MVDFDKPVSKNDILASLLLIADLNRPSLNQTLSWEQALKSFLSL
                     NDPLIKTEIFIKWLSQLNPDQLQSSPEIVLLLKHIVEQKLLPYTLKKILIQRENFTTS
                     QINWLCNHCLIVAPDQTTLPDIIKLGSWSWLENHMKRNELLNFDLLKIAILNKDYLAS
                     INNSKESQLDFIYALNANKFNYQQILELIALAQDSQIKSLLITCLLSKKEYLDNLKEK
                     SFLAHLNQSEFHSPPRLNPLIHQLNLSCLTVAQIELLQPEACVTLLCSIPHFHQLSEK
                     QVEKILLKYPKPDMIAYWLNHYSSRPNAHFILAHLMNLVDVHVVDELNKMDSTKKETI
                     IMNMVEHLELFDPTHPLLINQNEEKRLITAIQRYLYGQHHDAYVTYIKLLVYKLLNRN
                     YQFSPQAMQLLICLNNLEEFKELNNKIAYLTNYYLRASAQSGDSSLFYNDSRINITSM
                     TQLVQLAPKIPTKSKEKSLLSSIFCGIKISESSVMTPTNDIPEHPLINKLIKNKRTVK
                     SIDYFLMHYKGDKQVLSKIINDYLSYYVQEGCTEIHRRSIYHLIVLMSLSDISASVRE
                     AIFTAFLNYPELHDEQINFSLFVYDAKRTIRHFGAQASEDSYARVIDLCEGALKKLNP
                     NKHKEIISIATQALTEAKIELSFGENTGFIAQLISRLKRCWYYGWTGFFSPNPPTFVA
                     AIDSPIKPSEKKTSPLLQQRKYFTTKLETNLSIILDDIKSGYTQQKLNELIEALSIFS
                     LQSNPKNELKIRQKLHRLFHYLLLESKNDLTLDAWLIKNQQLLIANRFRLLEMQLMKG
                     ARNEVESLLKEFDEDSAHFSPISKEFTHSLPEQNPYKCESVSSNQTPGATILDSATEL
                     ATSLKSTLGNLGVFFSNNIMSAIYPNNKSTPQSTSTMISP"
     gene            3099..3404
                     /locus_tag="I9760_000916"
     CDS             3099..3404
                     /locus_tag="I9760_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HAT5042694.1"
                     /translation="MDERRKYFRLKNHGEINASLDNNSIEIVEISSNGAVVVKQKTDI
                     PKEGVLKLQIHNFIMELCYEVIRAEDNNIVLHFTKEDETNKLFLVLKRLRDERKNKT"
     gene            complement(3514..4359)
                     /locus_tag="I9760_000917"
     CDS             complement(3514..4359)
                     /locus_tag="I9760_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HAT5042695.1"
                     /translation="MNNLAQQIEALPITLAGRFIYRFLPYRRRLIFSNISQVYNDQLN
                     ESQKKRLAKAYYSHLAKSLKEALQLRFMSEKKLRAQVEVIGHEKMLAVVAQKKGVLVV
                     TGHFGNWEFAPLGGVLNFKEFKGQFHFIRRTLRFKFIERIMFKNYYQAGLNVIPKKNS
                     LEQVCVALEQNHAVIFVLDQHASLVNRDGIAVEFFGKKAGTYRSLATISRHTGIPVIP
                     AASYRLPNGKHVLEFHDPIPWKDYDTTQESLYRNTLAYNQALEKIILAHPEQWNWMHK
                     RWKLS"
     gene            complement(4352..5629)
                     /locus_tag="I9760_000918"
     CDS             complement(4352..5629)
                     /locus_tag="I9760_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HAT5042696.1"
                     /translation="MSQANRVFITGRSALTASGATADETWNAIISGQCGIDEINFWEL
                     SEWSHRLGGEIKDFQPSKMLPDRKLIKVISRQDIMGINAAVQAVEHSKMIAYRDSLAD
                     ADLFNEQTAIYVGSPGNKYFQQYDFIPLLAKTHGDMRSFANQLFDEVHPMWLLRILPN
                     NVLAYTGITYGFKGPNHNVTNHAVGGTQAILEAYHAIRSGQAERAVVVAYDIGTEPQG
                     LFYYEKLGVVSNRHLKPFDKEHDGTILAEGAAALVLESEASVKSRAATCYGEIVGGLS
                     ASEATGLFSIEADGQHLASLMGQTLSTSGINSEEIGLIVTHGNGNSKSDDSEALAIKS
                     VFKQEEVPATAFKWSMGHTLCASGVLDAVLTTYALETKCVPGIANFQQIAPTCEGLSV
                     SREHREIISNSYAMMINRGFASMNVCLVIKACE"
     gene            complement(5630..6922)
                     /locus_tag="I9760_000919"
     CDS             complement(5630..6922)
                     /locus_tag="I9760_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HAT5042697.1"
                     /translation="MKRRVAITGLGVVSPLGNDVSTTWMNLIAGHSGIDSIKQFDASA
                     FPTTIAAEVKNFSPDTSISGKHTRFIMSFTHFALEAARQAFEDASIFPTKQTSDRWGI
                     VTGSGMMTAEFEYLTRFQQACAAGGEIDWGKLQSNSRDFYNLVDFGKTTSNSGLSLLI
                     QQYGITGYASSVHTACASGGQALGLAMQVIRRGEADFMLAGGFDSMINPLGLSSFCLL
                     GALSTYNETPQTASRPFDATRNGFVLGEGAAFLILEEWSKAKARGAKIYAELAGEGNS
                     LSSYRITDSHPNGDGAIQAIKRALEDAGVQPRDVDYINAHGTSTKMNDLSETNAIKVV
                     FGDRIANLPVSSTKSQTGHLIAAAGALEAVLSVKSIEQAQVPKTANLKTPDPECDLDY
                     VVDGPREKSLGVVLSNSFGFGGSNSCLLFKHPEFEETK"
     gene            complement(6927..7823)
                     /locus_tag="I9760_000920"
     CDS             complement(6927..7823)
                     /locus_tag="I9760_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HAT5042698.1"
                     /translation="MRFLFVDRIVQLSPGEWIKGIKHVTRDDAYLTTDDHGRLCFMPS
                     LIGETLGQLAAWNVMQHNHFTARPVAGVVSSARLYRPAYVGETILLESNIDSLDEKAV
                     QYHSIAKVGNDIIFTIDGALGPMLPMTEFISTEVIRQQFAEIYRPGDWSSVLALSSPV
                     LIEENCSDCGMINSSMQFDRILASQPGISLIAEKRITRSASYFPDHFPKRPVLPMTVL
                     LECKLNLAKEFIARAKYDRAYRISELRKIKMNEFVHPGDVVVCQVKVKQQMDDELILS
                     YRCEVDGKRVCVVDIVLIPKGK"
     gene            complement(7823..8236)
                     /locus_tag="I9760_000921"
     CDS             complement(7823..8236)
                     /locus_tag="I9760_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAT5042699.1"
                     /translation="MNVAEVYPKVREIIADVLVIDEEEISLNSSLIEDLGAESIDFLD
                     LVFQLEKEFKIKIPRGQLEKNARGDLAEDEFEKGGILTTSGLQALKNYLSEVPADRFK
                     ENMKVNEIPVLFTVETFCKLVISAIAQQQATETVA"
     gene            complement(8307..9065)
                     /gene="fabL"
                     /locus_tag="I9760_000922"
     CDS             complement(8307..9065)
                     /gene="fabL"
                     /locus_tag="I9760_000922"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HAT5042700.1"
                     /translation="MSSLVFSGKVGLITGGARGIGKATALKLAQSGCDIAIVYYNSSD
                     EAQSLVNEITGMGRKAVALQADVADCQSVKEMFSQFREHFKHLNFLVSNAASGVLKPA
                     LKMSTKHWRWCLETNALALNHLVTEGLALMPKNSRVIALSSLGASRAIPNYAFIGASK
                     AALEALVRSLSLELASYGITVNTVSAGVVDTDALKHFPNREQLLDEYQAHALADRALT
                     PEDVANAIYLLCLPEAAMINAHTLYVDAGYSQVG"
     gene            9227..10594
                     /locus_tag="I9760_000923"
     CDS             9227..10594
                     /locus_tag="I9760_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042701.1"
                     /translation="MKYSWNKTVFPIAAIFSFRLLGLFLLIPVFTLYATNLKGATPTL
                     IGLALGSYGLAQGLLQIPFGMLSDKLGRKPMIAIGLLLFAGGSLLGAVTHSIAGMITA
                     RILQGAGAIGSVLIALLADLTLDEQRTKAMAIVGITIGTSFSLAMIISPALTHYYGLS
                     GVFYLTAALAVLGIMILYLIIPNPSKEQFHADSEPSLSLIKPVVTDQHLQRLNFGIFC
                     QHFILTATFFAIPMLLKYQIENGHLHQQWHFYLPLMLFSFILMVPFIIISERKKRLKT
                     VFVISVLLTTFTQGLLVLASQNWYWFCILMFVYFVSFNVLEATLPSLVSKLASPGSKG
                     TAMGIYSTSQFLGIFFGGSLAGILYQWYDNQAIFLINMILSCLWFIISLSMKSNVYFS
                     TLILPFTANNEESKLVVNQLLKVAGIKEVILSKDENTLYVRVDNEEYSTGSAEKILHQ
                     STIYQ"
     gene            10678..11157
                     /gene="ssb"
                     /locus_tag="I9760_000924"
     CDS             10678..11157
                     /gene="ssb"
                     /locus_tag="I9760_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAT5042702.1"
                     /translation="MARGINKVILVGNVGADPDVRYLPNGNAVTTLSVATSETWKDKT
                     TGEKQDRTEWHRVVCFNRLGEIAGEYIRKGSKLYVEGSLRTRKWQDQQGQDRYTTEIV
                     ASDIQMLDSKGSSATNYDDMPSFQGTSTPQQASTKNQATPTSTAQDAFDELDDDIPF"
     gene            complement(11225..11608)
                     /gene="rplQ"
                     /locus_tag="I9760_000925"
     CDS             complement(11225..11608)
                     /gene="rplQ"
                     /locus_tag="I9760_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAT5042703.1"
                     /translation="MRHRNSGRSFSRTSSHRKAMFSNMCCSLIEHELIRTTLPKAKDL
                     RRYIEPLITVSKSDSVASRRRAFDILRSKSAVGKLFTDLGPRFAKRPGGYIRIIKCGY
                     RDGDNAPMAIVELMDRPVSSDDTEE"
     gene            complement(11627..12619)
                     /gene="rpoA"
                     /locus_tag="I9760_000926"
     CDS             complement(11627..12619)
                     /gene="rpoA"
                     /locus_tag="I9760_000926"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAT5042704.1"
                     /translation="MYTEINEMLTPKVLKVQAESPYKARIVLEPLERGFGHTLGNALR
                     RILLSSMPGSAITEASIDGVLHEYSTIEGVQEDVVDLLLNLKSVAIKLTVGNEAQITL
                     NKEGPCQVTAGDIQLTHGQEIINPELVIANLNEKGKLNMTLKVERGIGFHNTDAFVRY
                     HDDEIEKKTVGKLKIDNSFSPVKKVAYFVDSARVENRTDLDKLTIELETNGTIDAEEA
                     IRISASILQRQLHAFVDMKFEESRADNKERNDFDPVLLRSVDDLELTVRSANCLKAEN
                     IHYIGDLVQRTESELLKTPNLGKKSLTEIKDVLASRSLSLGMKLENWPPASLGE"
     gene            complement(12638..13258)
                     /gene="rpsD"
                     /locus_tag="I9760_000927"
     CDS             complement(12638..13258)
                     /gene="rpsD"
                     /locus_tag="I9760_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAT5042705.1"
                     /translation="MARYLGPKCKLSRREGCDLLLKSGVRDHKSKCKSEKLPGQHGDK
                     KPRLNSYGIQLREKQKIRRLYGILEKQFRNYYKKAARQKGSTGENLMALLERRLDNVV
                     YRMGFASTRAEARQLVAHKAILVNDKVVNVPSFLVNPGDTVSVRQKAKNQGRIQAALA
                     LSEQRAPCDWITVDTGSFKGTFSTAPTLMDLSSDYNVNLVVELYSK"
     gene            complement(13275..13673)
                     /gene="rpsK"
                     /locus_tag="I9760_000928"
     CDS             complement(13275..13673)
                     /gene="rpsK"
                     /locus_tag="I9760_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAT5042706.1"
                     /translation="MAITKSKQQKTRKKVKRVVSDGIVHIHASFNNTIVTFTDRQGNA
                     LCWATSGGSGFRGSRKSTPYAAQVATERAAAVAKEYGMKSVAVFVHGPGPGRESTIRE
                     LITQDFKIVEITDVTGIPHNGCKPPKKRRV"
     gene            complement(13697..14053)
                     /gene="rpsM"
                     /locus_tag="I9760_000929"
     CDS             complement(13697..14053)
                     /gene="rpsM"
                     /locus_tag="I9760_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAT5042707.1"
                     /translation="MARIAGVNIPDHKHVVIALTAIYGIGKTTSLKLCKTVDIDPSVK
                     VSQLSDAQLESLRTEIAKITVEGDLRRVVTMNIKRLMDLGCYRGLRHRRGLPLRGQRT
                     KTNARTRKGRRKGTSS"
     gene            complement(14131..14244)
                     /gene="rpmJ"
                     /locus_tag="I9760_000930"
     CDS             complement(14131..14244)
                     /gene="rpmJ"
                     /locus_tag="I9760_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAT5042708.1"
                     /translation="MKVRASVKRICRNCKIIKRSGTIRVICKDARHKQKQG"
     gene            complement(14261..15589)
                     /gene="secY"
                     /locus_tag="I9760_000931"
     CDS             complement(14261..15589)
                     /gene="secY"
                     /locus_tag="I9760_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAT5042709.1"
                     /translation="MKNQKHNGSKPRGGLAELKSRLMFVVLGILVYRLGAHIPVPGLD
                     PAKLANFFSEQQNTIFGLFNMFSGGALSRVTVFAIGIMPYISASIIIQLFSVVSPKLE
                     QLKKEGESGRRKINQYTRYLTLLLSIFQSMGMARWLASQQIAMQPDFSFYFTAVVTLV
                     TGTMFLMWLGEQITEKGVGNGISLIIFSGIVSSMPNAIGSVLQQVKEGQMQALTLVII
                     AAVVIVVTGFVVFMERAQRRIRVNYAQRTQGRKVYAAQTSHLPLKINMSGVIPPIFAS
                     SIILLPATLAQFFSQTKGLGWLSDVGMALSPGQPLYLIVYAAAILFFAFFYAALVFNP
                     KDTADNLKKSGAYIPGIRPGEQTTKYIDSVMTRLTLVGAIYLVLVCLLPQILMYTWHV
                     PFYFGGTSLLIIVVVIMDFVAQVQAHLMTQQYDSLLKKANFRGTKLPGLL"
     gene            complement(15592..16026)
                     /gene="rplO"
                     /locus_tag="I9760_000932"
     CDS             complement(15592..16026)
                     /gene="rplO"
                     /locus_tag="I9760_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAT5042710.1"
                     /translation="MNLNTLSPDPGSRPSRRRVGRGIGSGLGKTCGKGHKGQKSRAGG
                     YHKINFEGGQMPIQRRLPKMGFKSRVGRTIDEVSLGELAKLNDEVIDLVALRKAGLIN
                     NSIKDVKVILSGELTAAIKLKGLRVTKGARSAIESLGGSIEE"
     gene            complement(16026..16211)
                     /gene="rpmD"
                     /locus_tag="I9760_000933"
     CDS             complement(16026..16211)
                     /gene="rpmD"
                     /locus_tag="I9760_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAT5042711.1"
                     /translation="MGKKIKITLVKSTIGRKPKHVAIAKQLGLGKTNSSVVHSDTPAI
                     RGLVNEINYLLLVEESA"
     gene            complement(16214..16720)
                     /gene="rpsE"
                     /locus_tag="I9760_000934"
     CDS             complement(16214..16720)
                     /gene="rpsE"
                     /locus_tag="I9760_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAT5042712.1"
                     /translation="MSFDELPKSDGYQEKLVSVTRTAKVVKGGRVFGFAVLVVVGDGK
                     GKVGFGRGKAREVPIAIQKAMDQAKKNMVYIPLSGTTIFHEITWNYGASKVFMKPASE
                     GTGIIAGGAMRAVLEVLGVQNILAKSIGSTNPSNIVRATIGALTHIGTPDYVAAKRGK
                     TVEEVMAG"
     gene            complement(16730..17089)
                     /gene="rplR"
                     /locus_tag="I9760_000935"
     CDS             complement(16730..17089)
                     /gene="rplR"
                     /locus_tag="I9760_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAT5042713.1"
                     /translation="MNKQNARHRRGLKAKALIRKTGRSRLVVYRSGVHIYSQIVKSDQ
                     LGDKVLVASSTIDKELRSSLTGKSKVEQASLVGQLLGKRAKAAGITQVAFDRAGYKYH
                     GRVKALAEGAREAGLDF"
     gene            complement(17100..17639)
                     /gene="rplF"
                     /locus_tag="I9760_000936"
     CDS             complement(17100..17639)
                     /gene="rplF"
                     /locus_tag="I9760_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAT5042714.1"
                     /translation="MSRVAKAPVIHSANVEVTFVDGVITVKGPKGILTQKINKLVNIQ
                     HSKESNKLEFSPASNDPMGWAQAGTARALVRNMVQGVTEGYTVTLELVGVGYRAQSKD
                     KSISLSLGYSHSIEYDLPKGVTVETPNNTTILLKGVDKQVLGQIASEIRAFRPPEPYK
                     GKGVKYAGEQIVRKEAKKK"
     gene            complement(17657..18052)
                     /gene="rpsH"
                     /locus_tag="I9760_000937"
     CDS             complement(17657..18052)
                     /gene="rpsH"
                     /locus_tag="I9760_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAT5042715.1"
                     /translation="MSMHDPIADMLTRIRNGQQAKHQQVTLVSSKLKEEIARVLKEEG
                     YIQDFFIETLPNGLKSITLKLKYYHGRPVIEFIKRISRPGLRVYKSYKDLHSIPGFGV
                     AILSTSKGIMTHVSAKVKGVGGEVICEVA"
     gene            complement(18075..18377)
                     /gene="rpsN"
                     /locus_tag="I9760_000938"
     CDS             complement(18075..18377)
                     /gene="rpsN"
                     /locus_tag="I9760_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAT5042716.1"
                     /translation="MAKKSMLMRESKRAKLVEKYRQRRNELKQLIKSSDDFQVIMESQ
                     AKLAKLPVNSNPVRYVTRCKQCGRPHAVYRKFNLCRICLRQQLMVGNIPGGRKSSW"
     gene            complement(18390..18941)
                     /gene="rplE"
                     /locus_tag="I9760_000939"
     CDS             complement(18390..18941)
                     /gene="rplE"
                     /locus_tag="I9760_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAT5042717.1"
                     /translation="MARLKEFYKKDVVTMMMKRFNYSSVMEVPRILKITLNMGVGEAV
                     GDKKVMNHAIEDMTLISGQKPVVTKARKSIAGFKIREGWPIGCKVTLRRERMYEFLDR
                     LISITLPRVRDFRGLNPKSFDGTGNYSMGIHEQIVFPEIDYDKTDGIRGLDICITTSA
                     KTNEEAKALLEAFNLPLKDKDRK"
     gene            complement(18957..19286)
                     /gene="rplX"
                     /locus_tag="I9760_000940"
     CDS             complement(18957..19286)
                     /gene="rplX"
                     /locus_tag="I9760_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAT5042718.1"
                     /translation="MKRIKSGDEVIVIAGKSKGHIGKVLRVIDDAVVVEGGNLIKKHI
                     KPNPQKPENKGGIIAREAPLHVSNVAHYNPVTKKADKVGFKYLESNGVSKKVRYYKSN
                     NEIIDRI"
     gene            complement(19299..19664)
                     /gene="rplN"
                     /locus_tag="I9760_000941"
     CDS             complement(19299..19664)
                     /gene="rplN"
                     /locus_tag="I9760_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAT5042719.1"
                     /translation="MIQMQTVLEVADNSGARKVMCIKVLGGSHRRYARVGDVIKVSVK
                     DAIPRSKVKKGAVMRAVVVRTAQGVRRDDGSLIRFDDNAAVLLNNQNEPIGTRIFGPV
                     TRELRERFMKIISLAAEVL"
     gene            complement(19753..20007)
                     /gene="rpsQ"
                     /locus_tag="I9760_000942"
     CDS             complement(19753..20007)
                     /gene="rpsQ"
                     /locus_tag="I9760_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAT5042720.1"
                     /translation="MSTNSESNARTMIGKVVSDKMDKTIVVMIERTVKHPKYGKIMKR
                     RTKLHAHDENQVCRVGNTVKIRESRPLSKTKSWVLVEVIS"
     gene            complement(20009..20203)
                     /gene="rpmC"
                     /locus_tag="I9760_000943"
     CDS             complement(20009..20203)
                     /gene="rpmC"
                     /locus_tag="I9760_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAT5042721.1"
                     /translation="MKKIDELRNMSVEELQNELLSLRKDQFNLRMKKASGSLDKTHLI
                     TMVRKSVAKVKTILTEKAGK"
     gene            complement(20203..20616)
                     /gene="rplP"
                     /locus_tag="I9760_000944"
     CDS             complement(20203..20616)
                     /gene="rplP"
                     /locus_tag="I9760_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAT5042722.1"
                     /translation="MLQPKRTKYRKQMKGRNRGLALRGSKISFGEFGLKAVERGRLTA
                     RQIEAARRAMTRHIKRGGKIWIRVFPDKPITQKPLEVRQGKGKGSVEYWVAQIQPGKV
                     LFEMEGVSKELAMEAFDLAKAKLPFKVMFEERTVM"
     gene            complement(20633..21289)
                     /gene="rpsC"
                     /locus_tag="I9760_000945"
     CDS             complement(20633..21289)
                     /gene="rpsC"
                     /locus_tag="I9760_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAT5042723.1"
                     /translation="MGQKVNPIGIRLGIIKDWNSKWFAGKRYAEFLIQDIKLRNDLKK
                     KLMAAAVSKILIERPANNAVVTILTARPGVIIGKKGGGIETLRKEISDNLGVPVHLNI
                     EEVKKPELDATLVAEGIAQQLEQRVMFRRAMKRAVTSALKAGAKGIKICVSGRLGGAE
                     IARSEWYREGRVPLHTFRADIDYGTAESKTTYGIIGVKVWIFKGEILPQKKRSTESAQ
                     "
     gene            complement(21292..21627)
                     /gene="rplV"
                     /locus_tag="I9760_000946"
     CDS             complement(21292..21627)
                     /gene="rplV"
                     /locus_tag="I9760_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAT5042724.1"
                     /translation="MEVTAKLKGAPLSAQKGRLVADMIRNMNVSGALDVLKFTPKKGA
                     KLMLKLLESAIANAENNNGADIDDLKVGMVCVDEATTLKRISPRAKGRANRICKRTCH
                     ITIKVSDEE"
     gene            complement(21637..21915)
                     /gene="rpsS"
                     /locus_tag="I9760_000947"
     CDS             complement(21637..21915)
                     /gene="rpsS"
                     /locus_tag="I9760_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAT5042725.1"
                     /translation="MARSIRKGPFIDHHLISKVEAAIESKSKKPIKTWSRRSTIVPEM
                     IDLTIAVHNGKDHVPVFITDNMVGHKLGEFAMTRTFKGHSGDRKAKGK"
     gene            complement(21934..22761)
                     /gene="rplB"
                     /locus_tag="I9760_000948"
     CDS             complement(21934..22761)
                     /gene="rplB"
                     /locus_tag="I9760_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAT5042726.1"
                     /translation="MALLKSKPTSPGKRGEIRVVHHDIYKGKPHAALVEKLKKTGGRN
                     NQGRITVRHIGGGQRQKYRIIDFKRNKDGILGRVERLEYDPNRTALIALISYKDGEKR
                     YIIAPSNLEVGATIQSGADSPISVGNCLPLKNIPVGTTIHCVEMKPGKGAQMLRSAGC
                     SGQLVAKEGVYATLRLRSGEMRKIHVLCRAVIGEVSNSEHNLRALGKAGAKRWRGIRP
                     TVRGVAMNPVDHPHGGGEGRTSGGRHPVSPWGLPTKGYKTRSNKRTDTFIVRGRKKK"
     gene            complement(22773..23069)
                     /gene="rplW"
                     /locus_tag="I9760_000949"
     CDS             complement(22773..23069)
                     /gene="rplW"
                     /locus_tag="I9760_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAT5042727.1"
                     /translation="MNAERLMMVLREPHTSEKATVMADKFKQFTFKVLKNATKTEIKL
                     AVEHIFNVKVKSVSVVNVKGKSKRFKQTSGKRSDWKKAFVSLHADQDIDFTVTE"
     gene            complement(23066..23671)
                     /gene="rplD"
                     /locus_tag="I9760_000950"
     CDS             complement(23066..23671)
                     /gene="rplD"
                     /locus_tag="I9760_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAT5042728.1"
                     /translation="MEITTIDTKSKLKLNKEIFAYTYNEGLVHQAVVTFMNNARSGNS
                     AQKTRSEVSGGGKKPWNQKGTGRARAGTIRSPLWRSGGVTFASKKRDYSQKLNKKMYK
                     RALRSIISELCRTGNLVVVSDFQCDNHKTKDFLKKMNQMEISNALIIMSEVGENEYLG
                     SRNLIDYDICDVTTIDPVSLLRFEKVVVTEAAIKKIEEQLQ"
     gene            complement(23671..24321)
                     /gene="rplC"
                     /locus_tag="I9760_000951"
     CDS             complement(23671..24321)
                     /gene="rplC"
                     /locus_tag="I9760_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAT5042729.1"
                     /translation="MMIGLLGRKIGMTRVFTPEGVSVPVSVVEVQPNRVSQVKTAAND
                     GYSAVQLTGGTKKSSKVSKPVAGHFAKAQIDAGDMQVEFRIDSEDAFTPGQVISVADV
                     FTAGQYVDVSGLTKGKGFAGTVKRHNFRTQDASHGNSRSHRVPGSIGQNQTPGRVFKG
                     KKMAGHMGNARCTIQSLELVKVDSERNLLLIKGAIPGAPGSRVEIKPAVKKQARGE"
     gene            complement(24356..24673)
                     /gene="rpsJ"
                     /locus_tag="I9760_000952"
     CDS             complement(24356..24673)
                     /gene="rpsJ"
                     /locus_tag="I9760_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAT5042730.1"
                     /translation="MSSNQNIKIRLKSFDHRLIDLSTREIVDTAKRTGAQIRGPIPLP
                     IRKEKFTVLTSPHVNKDARDQYELRTHKRLVVIVHPTEKTVDALMKLDLAAGVDVQIS
                     LDD"
BASE COUNT         7395 a         5408 c         3977 g         7897 t
ORIGIN      
        1 aggacttagg aaaaacgcta aggtcaaggc aaaaaatatt tttaatgagg gagtttagat
       61 aaactaaatg accgaattaa aaactggttt ttacgcaaag attcggggtt gcttaagtcc
      121 tgtaaagatt ccatcgagag aatttgtgaa tattatattc cgtggcttaa catgaacgaa
      181 atgggtatta aagttgatag agtagttttg acatttcggt caaggaggat caagtggttg
      241 attttgataa acctgtctca aaaaatgata ttcttgccag cttattactt atcgcagatt
      301 taaatagacc atctctaaat caaactcttt cctgggagca agctctaaaa tcatttttat
      361 cgcttaatga tccgctgatt aaaacggaaa tatttataaa atggctcagt caattaaatc
      421 ctgatcaact tcaatcgtcc ccagagatag tcctcctttt gaaacacatt gtcgagcaaa
      481 agctgctccc ctacaccctc aaaaaaatat tgattcagcg tgaaaacttc accacctcac
      541 aaatcaattg gctttgcaat cattgtctta tagttgcacc tgatcaaacg actttacctg
      601 atattattaa actcggctca tggagctggc tggagaatca tatgaaaaga aatgaactat
      661 taaattttga tttgttaaaa atagccatct taaataagga ttatttagcc agcatcaaca
      721 atagtaaaga aagtcagctc gattttatct atgcgttaaa cgctaataaa tttaactacc
      781 aacaaatact tgaattaatt gctttagcgc aggatagcca gattaaatca ttattaatta
      841 cctgtctatt aagcaaaaag gagtacctgg acaatttaaa agaaaaatca tttctagccc
      901 atttaaatca aagtgaattt cactcaccac ctcggcttaa tccactaatc caccaattaa
      961 acctgagctg tttgactgtg gcacaaatag aacttctaca acctgaagca tgtgttactt
     1021 tactctgttc aatccctcat tttcatcagt tgtcagaaaa acaggttgaa aaaatactac
     1081 ttaaatatcc aaagcctgac atgattgcct attggctaaa tcattactca tccaggccaa
     1141 atgcgcattt tattttagcc catttgatga acctggtaga tgttcatgtt gttgacgaac
     1201 ttaataaaat ggattccaca aaaaaggaaa ccattattat gaatatggta gaacatctcg
     1261 aattatttga tccgactcat cctcttttga tcaaccaaaa tgaagaaaaa cgactaatta
     1321 ccgctattca acgctatctc tatggccaac atcatgacgc gtatgtaact tacatcaagt
     1381 tattggttta taaattatta aaccgtaatt accaattctc acctcaagcg atgcaactcc
     1441 tgatttgttt aaataatctg gaagagttta aagagttaaa taataaaatc gcctatctga
     1501 ccaattacta cttgcgagca tcagcacaat ccggtgattc gagcttattt tataatgatt
     1561 cacgcataaa catcacgagc atgacacaac tggtccagct agccccaaaa attccaacta
     1621 aatcaaaaga aaaaagtctt ttaagcagca tcttctgcgg catcaaaatt agcgaaagct
     1681 ctgtaatgac accgaccaat gatattcctg aacatccttt aattaataaa ttaataaaaa
     1741 acaagagaac agtgaaatcg atagattatt ttttaatgca ttataaaggc gataagcaag
     1801 tactttccaa aatcatcaat gattatttaa gttactatgt tcaagaagga tgcactgaaa
     1861 tacatcggcg ttcgatatat catttaattg ttctcatgtc tttaagcgat atatcagctt
     1921 ctgttagaga ggctatattc acagcgtttc taaactatcc tgaattacat gatgagcaaa
     1981 ttaatttttc tttatttgtc tatgatgcga aaagaactat ccggcatttt ggggcacaag
     2041 ctagcgaaga cagctatgcc agagttattg atttatgcga gggagctcta aaaaaattaa
     2101 atccaaataa acacaaggaa attatcagta tagcgactca agctttaact gaagcaaaaa
     2161 tcgagttaag ttttggtgaa aataccggat ttatcgctca actgatttca cgattgaaac
     2221 gatgttggta ttatggctgg actggttttt tttcccccaa cccacctacc tttgttgcag
     2281 caattgactc gccaataaag ccaagcgaaa aaaaaacctc ccctcttctt caacaaagaa
     2341 aatatttcac taccaaactt gaaacaaatc tgtcgattat cctcgatgac ataaaatcag
     2401 gttacaccca acaaaaactt aatgaattaa tcgaagcatt gagtatattt tcattgcaat
     2461 ccaatccaaa aaacgagctt aaaattcgtc aaaaactcca tcgcttattt cactatctgt
     2521 tactagaaag caaaaatgac ttaacattgg atgcctggct tataaaaaat cagcaacttt
     2581 taatcgcgaa tcgttttcgt ttgctagaaa tgcaactaat gaaaggagct cgcaatgagg
     2641 ttgaatcttt actcaaggaa ttcgatgaag actctgctca tttcagccct atcagcaaag
     2701 aatttactca ttctttaccc gaacaaaatc cgtataagtg tgaatctgtt tcttcaaatc
     2761 aaactccagg cgcaactatc ctggattcag caactgagtt agctactagc ctaaaaagca
     2821 cattaggcaa cttgggagta ttttttagca acaacatcat gtcagctatt tatccaaata
     2881 ataaatccac tccccaatcg acatcaacaa tgatttcacc ttgattaatc ttccggctgc
     2941 gcatctacag ccggattgat ttataataac ctgaaacaaa caaccgttct aaaatttgga
     3001 tccttcctat ttaattagca gatatcacgc tcatccggtt gacctatcag gaataataga
     3061 cctaaaatta aaaagcatac ttaacttatg gttaaacaat ggacgaaagg cgaaaatatt
     3121 ttcggctaaa aaaccatggc gaaataaatg ccagcttaga caataattca attgaaattg
     3181 ttgaaatctc ttccaatggt gctgtagtag ttaagcagaa aactgatatt cctaaagagg
     3241 gagtgcttaa actacaaatc cataacttca ttatggaact ttgctatgaa gtgattcgag
     3301 ctgaagacaa caacattgtc cttcatttca caaaagaaga tgaaacgaat aagttatttc
     3361 tggtactcaa acgattaaga gacgaacgaa aaaataaaac ataaaaattt gacataactt
     3421 gcccccataa aaagtcattt gtgattaagt aggagcctgg agaaaacatt ctgtgacaaa
     3481 gaataaataa gaaaccgaag acaatccatt catttaagaa agtttccagc gtttgtgcat
     3541 ccaattccac tgctcaggat gtgccagaat aatcttttcc aaagcctgat tataggccaa
     3601 ggtatttcgg tataaagatt cttgtgtagt gtcataatct ttccagggaa tggggtcatg
     3661 aaactccaaa acatgctttc cattaggtaa tctgtaactg gctgcaggta taactggaat
     3721 tcctgtatgg cgagatatcg tcgccaggct tctgtaggtg ccagcttttt ttccaaaaaa
     3781 ctctaccgct atcccatctc tgttcaccag ggatgcatgc tgatccaata cgaagattac
     3841 ggcatgattt tgttctaacg caacacaaac ctgctccaga gaattttttt taggaataac
     3901 atttaggcca gcttgatagt aatttttgaa cataatgcgt tctatgaact taaatcgcaa
     3961 ggtacgcctg atgaaatgaa attggccttt gaattccttg aagtttaata cccctcccaa
     4021 gggcgcaaac tcccaatttc caaagtgacc ggtaacgact aacactcctt ttttctgtgc
     4081 cacaacagct aacatttttt catggccgat tacttcaacc tgagctcgta attttttttc
     4141 actcatgaat cttaattgca gcgcttcttt taaagatttt gcaagatgtg agtaatacgc
     4201 ttttgccaag cgcttttttt gagattcatt cagttgatca ttataaacct ggctaatatt
     4261 ggaaaaaatc aaccggcgcc tgtatggcaa aaaccgataa ataaaacgtc ccgccagtgt
     4321 aatgggtaat gcttctattt gttgagctaa attattcaca agctttgatc accaaacata
     4381 cattcatgct ggcaaaccct cggttgatca tcatggcata ggaattagat attatctctc
     4441 tatgctcacg acttactgat aagccttcac acgtcggggc aatttgttga aaattagcga
     4501 tccccggcac gcatttggtt tcaagggcat atgtagttaa taccgcatcc aatactccag
     4561 aggcgcataa agtatgtccc atggaccatt taaaagcagt cgcaggaact tcttcttgtt
     4621 tgaatactga tttgatagcc agagcctcac tgtcgtctga tttactgttt ccatttccat
     4681 gggttactat tagtccaatt tcctcagagt ttattcctga tgtgcttaat gtctgcccca
     4741 tcaatgaagc caaatgttgc ccatctgctt caatagaaaa taatcctgtt gcttcagaag
     4801 cggataagcc accaactatc tcaccataac aagtcgcagc cctggacttc acactggctt
     4861 cactttccaa tacaagggct gcggcacctt cagcaagaat ggtgccgtca tgctctttat
     4921 caaaaggttt taaatgacga ttacttacca cacccaattt ttcataataa aataagcctt
     4981 gaggttctgt gcctatatca taggcaacca caaccgcacg ttctgcctgc ccgctacgta
     5041 tggcatgata cgcttccaaa atggcttgtg taccacctac cgcatgattt gttacattgt
     5101 gatttggacc tttaaagcca taggtaatac ctgtataggc aagcacatta ttaggtaaaa
     5161 tcctcaataa ccacatagga tgcacttcat caaacaactg atttgcaaag ctacgcatat
     5221 ctccatgggt tttagccaac aaaggtataa agtcatattg ttgaaaatat ttattccctg
     5281 gagaaccaac ataaatagcg gtttgttcat taaataaatc agcatctgcc aggctatctc
     5341 tgtaagcaat cattttacta tgctcaacag cttgcactgc cgcattgatt cccattatat
     5401 cttgacggga aataactttt attaattttc tatcaggaag catcttggaa ggttggaaat
     5461 cttttatttc tccgcccaaa cgatgcgacc attcagataa ttcccaaaaa ttaatttcat
     5521 ctattccaca ctggcctgaa atgattgcat tccaagtttc atcagcagtt gcaccagagg
     5581 cagttagagc gctgcgacca gtaataaata ctctattcgc ttgactcatt tattttgtct
     5641 cctcgaattc tggatgttta aataacaagc agctattaga acctccaaaa ccaaacgaat
     5701 tagataatac tacccctaaa gatttttcac gtggaccatc aacaacataa tccaaatcac
     5761 actccggatc aggtgttttt aaattcgctg ttttggggac ttgcgcctgt tctatggatt
     5821 taactgacaa taccgcttca agcgctcccg cagctgcgat taaatgcccc gtttgacttt
     5881 tggtagagct cactggtaaa ttggcgatcc tgtccccaaa gacaactttg attgcattgg
     5941 tttcacttaa atcattcatt ttagtggatg taccatgcgc attaatataa tcgacatccc
     6001 gtggttgaac acccgcatcc tccaacgcac gtttaatcgc ttgaatagca ccatcaccgt
     6061 tgggatgtga atcagttatt cggtaggagc tcaaagaatt cccctcaccg gccagttcag
     6121 catagatttt tgccccacgg gcttttgcct tactccattc ttcaaggatc aaaaaggcag
     6181 ccccttcacc caggacaaaa ccattgcgtg tcgcatcaaa ggggcgactt gccgtttgcg
     6241 gagtctcatt atacgttgat aaggcaccca aaagacaaaa gctcgacaaa cccaaaggat
     6301 taatcatgga atcaaatcca cctgccaaca taaaatctgc ctcgccacga cggatcacct
     6361 gcattgccaa tcccaaggct tggcctcctg aagcgcaggc cgtatggaca gaagaggcat
     6421 aaccagtaat cccatattgt tgtattaata aggaaagacc agagtttgaa gtggttttac
     6481 caaaatcaac caaattatag aaatccctgc tattagattg cagctttccc caatcaattt
     6541 caccgcctgc ggcacaagct tgttgaaacc gcgttaaata ctcaaactcg gccgtcatca
     6601 tcccactgcc tgttactatt ccccaccgat cagatgtttg cttggtagga aatatagaag
     6661 catcttcaaa tgcctggcgt gcagcttcca aagcaaaatg agtaaaagac atgataaatc
     6721 gagtatgctt tcccgaaata gacgtatcag gtgaaaagtt ctttacttca gccgcaatag
     6781 tggtaggaaa agcactggcg tcaaactgct tgatactatc tatgccagaa tgaccagcga
     6841 tcaaattcat ccaagtggtg gatacatcat ttcctagagg cgaaacaacc cctagacctg
     6901 taatagcaac tcgtcgtttc ataacctcat ttaccttttg ggataagcac tatgtcgaca
     6961 acacataccc gcttgccatc aacctcacac ctataagaca gtattaattc atcatccatt
     7021 tgctgtttca ctttgacctg gcaaactacg acatctccgg gatgaacaaa ttcattcatt
     7081 ttaattttac gtaattcgct gattcgataa gctctatcat attttgctct agctataaac
     7141 tctttcgcca aattcaattt acattcaaga agtaccgtca tcggtagtac aggtctttta
     7201 ggaaaatgat ccgggaaata agatgctgaa cgagttatcc ttttttcagc aatcaagctt
     7261 atacctggtt gactggcgag gattctgtca aattgcatgg aagaatttat cattccacaa
     7321 tcagagcagt tttcctctat taaaacaggc gaactcaaag ctaacacaga agaccagtca
     7381 cctggccggt atatttctgc aaactgctgt ctaatgacct ctgtactaat aaattcagtc
     7441 ataggcagca tggggcctaa tgctccatct atagtaaaaa taatatcatt acctactttg
     7501 gcaatgctat ggtattgaac tgccttttca tccagtgaat caatatttga ttcaagcaat
     7561 atcgtttcac cgacgtaagc aggtcgatac aatcgggcac ttgatactac gccagcgact
     7621 ggtcttgcag taaaatgatt atgttgcatc acattccacg ctgccaattg ccccaatgtt
     7681 tcaccaatca aagaaggcat aaagcaaagc cttccatgat catcagtggt taaataagca
     7741 tcatctcttg tgacatgctt tatcccttta atccattctc ctggggataa ttgtacaatg
     7801 cgatcaacaa ataagaacct catcaggcta cagtttccgt tgcttgctgc tgggctatag
     7861 ctgaaataac cagcttgcaa aaagtttcta cggtaaacaa cacaggaatt tcgttaactt
     7921 tcatattctc tttaaatcga tcagcaggga cttcgctcaa ataattcttt aaagcctgta
     7981 atcctgaggt tgttaaaata ccacctttct caaactcatc ttcagccaga tcaccacgag
     8041 cattcttttc caactggcct ctcgggattt taatcttgaa ttctttttcc aattgaaaaa
     8101 ccaaatcaag aaaatcgatg gactcagcac ccaaatcctc aattaaacta ctgtttaaag
     8161 aaatttcttc ttcatcaatt actaatacat cagcaataat ttccctaacc ttagggtaaa
     8221 cttcagctac attcatattt tatcctctga accaacaaag ctcgcaaagc ttgaaattat
     8281 atcataaaat ttacttttat catctactag ccaacctgac tatagcctgc gtcaacatat
     8341 aatgtatggg cattaatcat ggccgcttca ggcaaacaaa gcaaatagat agcattagcc
     8401 acatcttcag gtgttaacgc tctatctgcc aaagcatgag cttgatattc atccagtaac
     8461 tgttctctgt ttggaaaatg ctttaaagca tccgtatcaa caacacctgc agaaacagta
     8521 ttcacagtaa taccataact agccagctcc aaacttagcg aacgaaccaa tgcttccaga
     8581 gctgctttag aggcaccaat aaaagcataa tttggaatcg cccttgatgc acctaagctg
     8641 gatagtgcaa taactcgact gtttttaggc attagagcaa gaccttccgt aaccaagtgg
     8701 ttcaaagcca aagcattggt ttccaaacac cagcgccaat gcttggtcga cattttcaaa
     8761 gcaggtttca atactccact cgctgcattg ctcactagaa aatttaagtg cttaaagtgt
     8821 tctctaaatt gagagaacat ttctttgaca gattgacaat ctgctacatc ggcttgtaag
     8881 gcaactgctt ttctacccat gccggttatt tcattgacca gactttgagc ctcatcagag
     8941 ctattataat acacgatagc aatatcgcag cctgactgtg ctaactttaa tgcggtcgct
     9001 ttaccaattc ctcgtgcacc acccgttatc aatcctacct ttcctgaaaa aactaatgaa
     9061 ctcatacgat aaccctatag tataaaatcc caccaaacta ccgctaatct gctgtgctgt
     9121 caatggaaat atcgggactc tatcaagcgg aatttcataa tttatcttgt ttccaaagca
     9181 cttaatttgt accatctcca catgatataa tttagaccta taatgaatga agtattcttg
     9241 gaacaaaact gtttttccta tagcagcaat attctctttt cgtctcctgg gattattttt
     9301 acttatccct gtattcactc tttatgccac taaccttaaa ggtgctactc caacattaat
     9361 tggcttggct ttgggaagct atggattggc acaaggatta ctacaaatcc cttttggtat
     9421 gctttcagat aaactaggca gaaaacctat gatcgccata ggtctattgc tattcgctgg
     9481 aggaagcttg ttaggagcag taactcattc tattgctgga atgataactg ccagaatact
     9541 acagggtgct ggagctatag gtagcgtgtt aattgctttg cttgctgatt taaccctgga
     9601 tgaacaacgt acaaaggcaa tggccatagt agggataact attggaacat catttagcct
     9661 cgcaatgata attagccctg ctcttaccca ctactatgga ttatcaggag tgttctatct
     9721 cactgcagct ttagctgttc tagggattat gatactttat ctcatcattc ccaatccctc
     9781 aaaggagcag tttcatgcag atagtgaacc cagcttatct ctcataaaac cagtggtaac
     9841 agatcaacat ttacaacgcc tcaattttgg aattttttgt cagcatttta ttcttacagc
     9901 cacttttttt gctataccca tgctattgaa atatcaaatt gagaatggtc atttacatca
     9961 gcaatggcac ttctatttgc cactcatgtt attctcattt attttaatgg taccctttat
    10021 cattatttca gaaagaaaaa aacgattaaa aacagtattt gttatatctg tactgctgac
    10081 tacctttact caaggtctgc tggtactcgc atcacaaaat tggtattggt tttgtatttt
    10141 aatgtttgtg tatttcgttt ccttcaatgt tcttgaagca acattacctt cattagtgtc
    10201 aaagctggca agtccaggga gcaaaggcac agcaatgggt atttattcaa cgagtcaatt
    10261 tttaggaatt ttttttggtg ggtctttagc tggtattctt tatcagtggt acgataacca
    10321 agctattttt ctaattaaca tgattctgag ctgtctatgg ttcatcattt cgctaagcat
    10381 gaaatctaat gtttattttt ctactcttat tttgcctttt acagcaaata atgaagaatc
    10441 aaaattggta gtcaatcagc ttttaaaggt agccggcatt aaagaagtta ttttgtccaa
    10501 agatgaaaat accttgtatg ttcgagtaga taatgaggaa tactccacag gaagcgctga
    10561 aaaaattctt catcagtcta caatttacca ataacacctt ctctttgggc tgctacgaaa
    10621 taaccgccaa attatgtata atacctaact aactttcgca tacaggagat gatttcaatg
    10681 gcaagaggaa taaataaagt aatacttgtt ggtaatgtag gggcagatcc tgatgtaagg
    10741 tatttgccta acggaaatgc tgttactaca ctttcagttg ctaccagtga aacatggaaa
    10801 gacaaaacga caggtgaaaa acaagataga actgaatggc acagagtagt ctgttttaat
    10861 cgtctgggtg aaatagccgg agaatacatt cgcaaaggtt ccaagctata tgtcgaaggg
    10921 agcttaagaa caagaaaatg gcaagatcaa cagggccaag atagatatac aactgaaatt
    10981 gttgcgtctg acattcaaat gctcgatagc aaaggaagta gtgccactaa ttacgacgac
    11041 atgccttcat ttcagggaac ctctacccct caacaagctt caacaaaaaa tcaagcaact
    11101 ccaacatcta ctgcacaaga tgcttttgat gaattagatg atgacatacc gttttaaatg
    11161 attcaaataa aagcccgcgt taatgcgggc tcaaaatata cattaatagt tattaatcta
    11221 aaatttactc ttccgtatca tcactgctta caggtctatc cattaattca actatagcca
    11281 taggtgcatt atcaccatcc ctatacccgc atttaataat acgtatgtac ccacctggtc
    11341 gttttgcgaa acgtggtcct aaatcagtaa ataatttacc tacagctgat ttagatcgca
    11401 aaatatcaaa agcacgtcta cgtgatgcta ctgaatcaga tttactaaca gtaatcaaag
    11461 gttcgatata tcgacgtaaa tcttttgcct ttggcaaagt ggttctaatt aactcgtgct
    11521 caatcaaaga acaacacata ttagaaaaca tagccttacg gtggctactg gtacggctaa
    11581 aactacgtcc ggaattacgg tgacgcataa caaagactcc taaatattac tcgccaaggc
    11641 ttgctggcgg ccaattctca agtttcatac ccagcgataa tgatcgagac gctaaaacgt
    11701 ctttaatttc agttagtgat ttcttaccaa gattaggtgt cttcaataat tcactttctg
    11761 ttctttgaac caaatcgcct atataatgaa tgttttcagc ttttaaacaa ttagctgatc
    11821 gaacagtcaa ctccaaatca tccactgaac gtaataatac aggatcaaaa tcattacgct
    11881 ctttattgtc agcacgtgat tcttcaaatt tcatgtcaac aaacgcatga agctgtcttt
    11941 gtagtatact agcagatatt cttattgcct cttcagcatc gatagtaccg ttagtttcta
    12001 attcaatagt aagcttatct aaatctgttc tgttttctac acgtgcactg tcgacgaaat
    12061 aagctacttt cttcactgga gaaaaactgt tgtcaatttt taactttcca accgtctttt
    12121 tctcaatttc atcatcatga tagcgaacaa atgcatcagt attatgaaag ccaatacctc
    12181 tttcaacttt aagagtcata tttaatttac ccttttcatt gagattggct atgacaagtt
    12241 ctggattaat aatttcttga ccatgagtca actgaatatc accagctgta acttgacatg
    12301 gtccttcttt atttaaggta atctgcgctt cgtttcctac agtcaatttg atagcaactg
    12361 attttaggtt aagcaacaaa tcaaccacat cttcctgaac accttcaata gtactatatt
    12421 catgcaaaac accatcgata gaagcttcag taatggcact tcctggcatg gatgacaata
    12481 agattcttct caaagcattg ccgagggtgt gaccaaaacc tcgctccaaa ggttctaaaa
    12541 ctattctagc tttatagggc gactcggcct gtactttgag aaccttaggc gtcaacattt
    12601 cattgatttc agtatacatt cagctatctc tccgagctta cttagagtaa agttctacaa
    12661 caaggtttac gttgtagtct gaagataaat ccattaacgt cggtgctgta gaaaatgtac
    12721 ctttaaaaga accagtatct acagtaatcc aatcacaggg tgctctttgt tcagataaag
    12781 ctaaagcagc ttggatacgt ccttggtttt tagccttttg tcgaactgat acagtatccc
    12841 caggattaac cagaaaagaa ggtacgttaa ctactttatc atttacaagt attgccttat
    12901 gtgcaactag ttgtctagct tcagcacgtg tactggcaaa acccatacgg taaacaacat
    12961 tatctaaacg tctttccaat agggccatta aattttcacc tgtagagccc ttttgtctag
    13021 ccgctttttt gtaataatta cggaattgct tttcaagaat cccataaagc cttctaatct
    13081 tttgtttctc tctaagctga attccataac tatttaaacg aggtttttta tcaccatgct
    13141 gtccaggtaa cttttcagat ttacacttgg atttatgatc acgaacccca cttttaagta
    13201 ataaatcaca accttcgcgt cgtgataatt tacatttagg accaagatat ctagccatta
    13261 attactcctg agtcttatac acgacgtttt ttaggtggct tacaaccatt atgtggtatg
    13321 ccagttacat cggtaatttc aacaatttta aaatcctggg ttattaattc acgaatagta
    13381 gattcacgac caggaccagg accatgcaca aaaactgcaa cagatttcat accatattct
    13441 ttagcaacag cagcagctcg ctcagttgca acttgcgcag cataaggagt actctttcta
    13501 gaacctctga agccagatcc accagatgtt gcccagcata atgcattacc ttgtctatct
    13561 gtaaaggtaa ctatagtatt gttgaaagat gcatgaatgt gtactatgcc atcagacact
    13621 acgcgtttta ccttttttcg tgttttttgc tgctttgact ttgttattgc catcttttat
    13681 tttcctacat gaaaaatcaa ctactagtgc cttttctacg gccttttctg gtacgagcat
    13741 tagttttcgt acgctgtccg cgcaacggca atcctctcct gtgtcttaat cctctataac
    13801 aaccaagatc cattagtctt tttatgttca ttgttacaac acgtcgaaga tcaccctcta
    13861 cagtaatttt cgcaatttca gttctcaaag attccaactg agcatctgat aattgagaaa
    13921 ctttaactga aggatctata tcaactgttt tacataattt caatgatgtt gttttaccaa
    13981 taccataaat tgctgttaaa gcgattacca catgtttatg atcaggtata ttaactcctg
    14041 caatacgagc cattagtttc tccgaacgtt atttgttctg tcgaaaggga cagaaggata
    14101 ctattttaat aaaataaaat caagcagata ttacccttgc ttttgtttat gacgggcatc
    14161 cttacaaatt actcgtatag tgccacttct ttttattatt ttacaattgc gacatattcg
    14221 ctttactgat gctcttactt tcattcctaa ctccgataaa tcatagcaga ccaggtaact
    14281 tagtacctct aaaattcgcc ttttttaata aagaatcata ttgctgagtc attaaatgag
    14341 cttgtacctg tgctacaaaa tccataataa caacaactat aatcagcaac gaagtaccac
    14401 caaaataaaa tggaacatgc caggtataca ttaaaatttg tggtaacaaa catactaaaa
    14461 ccaagtaaat ggctccaact aaagtcaaac gagtcatcac tgaatcgata tatttagttg
    14521 tttgctctcc tggacgaatt ccaggaatat atgctcctga ttttttcagg ttatctgctg
    14581 tatccttagg gttaaacact aaggcagcat aaaagaatgc aaagaacaaa atagctgctg
    14641 catacactat caaatataag ggttgacctg gtgatagtgc cattccaaca tcagataacc
    14701 atcccagccc tttagtttgt gaaaaaaatt gagctaaagt tgctggtaat aaaataatgc
    14761 tagatgcaaa tattggtgga atcacacctg acatatttat ttttaatggt aaatgactcg
    14821 tttgggctgc atataccttt ctaccttgcg ttctctgtgc ataattaaca cgaattcgtc
    14881 gttgagcacg ctccataaat acaacaaaac cagttactac aattactact gctgcaatta
    14941 taactagtgt aagagcttgc atctgacctt ccttaacttg ctggaggaca gatcctatag
    15001 catttggcat gctagagaca attccagaaa agataattaa ggaaattcca ttaccaacac
    15061 ctttttctgt aatttgctca cccaaccaca ttaaaaacat agtgcctgta actaaagtta
    15121 caacagcagt aaaatagaat gagaaatctg gttgcatagc aatttgctga ctagccaacc
    15181 atctcgccat acccatagac tgaaatattg acaagagcaa tgttaaatat ctggtatatt
    15241 gatttatttt tctacgacct gattcaccct ctttctttag ttgctccagc ttgggagaaa
    15301 ctacagaaaa cagttgaata ataattgatg cagaaatata aggcataata ccaatcgcaa
    15361 acactgttac acgagacagg gctccaccag aaaacatatt aaacaagcca aatatagtgt
    15421 tttgctgttc actaaaaaaa ttagctaatt ttgcaggatc taatcctgga acaggtatat
    15481 gagcacctaa tcggtaaact aatatgccta gaacaacaaa catcagtctt gactttagtt
    15541 cggctaatcc accacgaggc ttactaccgt tatgcttttg gtttttcata gtcactcttc
    15601 tatactaccg cctaaactct caatagcaga acgagctcct ttagtgactc ttaaaccctt
    15661 aagcttaatt gctgcagtca gctcgccaga taaaattact ttaacatctt tgattgagtt
    15721 attaattaaa cctgctttac gtaaagcaac taaatcaatt acttcatcat tcaattttgc
    15781 aagctcaccc aaactcacct catcaatggt tcgacctact ctagacttga aacccatttt
    15841 gggtaatcgt ctttgtatag gcatttgacc accttcaaaa ttaattttgt ggtaaccacc
    15901 tgctcttgat ttttgacctt tatgaccttt gccacaagtt ttgcctaatc cagaaccaat
    15961 acctctcccc actcgtcttc ttgagggtct ggaacctgga tctggcgata atgtatttaa
    16021 attcattatg cactctcctc aaccaacaac aggtaattaa tttcatttac aagtccacga
    16081 attgcaggag tatcactatg aaccacactt gaatttgttt tacctaaacc cagctgtttg
    16141 gctatagcaa catgctttgg ttttctacct atcgtgcttt taaccaaggt tattttaatt
    16201 ttttttccca taattagcct gccattactt cttcaactgt ttttccacgt ttggctgcta
    16261 catagtccgg cgtaccaatg tgtgtcaatg caccaatagt ggcacgaaca atgttacttg
    16321 gattggtaga accaatgctt tttgcaagaa tattttgcac acccaatact tccaaaacgg
    16381 cacgcatagc acctccagca ataattccag taccttcact tgctggcttc ataaacacct
    16441 tggaagctcc atagttccat gtgatttcgt ggaatattgt agtacctgaa agaggaatgt
    16501 aaaccatatt tttcttggct tggtccatag ctttttgaat tgctattgga acctctcttg
    16561 ccttacctcg tccaaaacct actttaccct ttccgtcacc tacaactact aaaacagcaa
    16621 aaccaaaaac tcgtccaccc tttacaactt tagccgtacg agttaccgat accaattttt
    16681 cttggtatcc atctgacttg ggtaattcat cgaatgacat aatcgttcct taaaaatcta
    16741 atccggcttc gcgtgcacct tcagcaagtg ctttaactct gccatgatat ttataacctg
    16801 ctctgtcaaa tgcaacctgt gtaatgcctg cagccttagc acgcttaccc aatagttgac
    16861 caactaaact ggcttgttca actttacttt ttccagtcaa actggatctc aattctttat
    16921 ctatagtaga tgaagctacc aacaccttat caccaagctg atctgacttg acaatttgag
    16981 aataaatatg cacaccactt cgatacacaa ctaatcttga gcggcctgtt ttgcgtatta
    17041 aggctttggc ttttaatcca cgtctatgtc gtgcgttttg cttattcata gttaacacct
    17101 tatttcttct tagcttcttt acgaacaatc tgctctccag catatttcac acctttccct
    17161 ttataaggct ctggtggtcg aaatgctcta atttcagaag ctatttgtcc aagaacttgc
    17221 ttatcaacgc ccttcagcaa aatggttgtg ttattaggag tctctacggt tacacctttt
    17281 ggtaaatcat actctataga atgtgagtat cctaatgaca aactaattga cttgtcttta
    17341 gattgcgctc tatatcctac accaaccaat tctagtgtaa ctgtatatcc ctcagtaact
    17401 ccttgaacca tgtttctaac taaggctcta gctgtaccag cttgtgccca tcccattgga
    17461 tcattagaag caggtgaaaa ttctaatttg tttgattcct tactatgttg aatattaacc
    17521 aatttattaa ttttttgagt taatattcct ttggggcctt ttacagttat tactccatca
    17581 acaaatgtta cttcgacatt tgctgaatga attactgggg cctttgctac tctagacata
    17641 tcattcctcg caagtattaa gccacttcac aaatgacttc gccaccaaca ccttttactt
    17701 ttgctgatac atgggtcatt atacctttag acgtagaaag aatagccaca ccaaatccag
    17761 ggattgaatg caaatcctta tatgatttat atactctcaa acctggacga ctaatgcgct
    17821 taataaattc aattaccggt cttccatgat agtactttag ctttaaagta attgacttca
    17881 gaccatttgg taatgtttca ataaaaaagt cttgtatata tccttcttcc tttaaaactc
    17941 gagctatttc ttcttttaat ttagaagaaa ccaatgttac ttgctgatgt ttagcttgtt
    18001 gaccatttct aattctagtc agcatatcag caattggatc atgcatactc acaattattc
    18061 tccaataaaa tagtttacca gcttgatttt ctaccgccag ggatattgcc caccataagc
    18121 tgttgtctta aacaaattct gcaaagatta aacttgcgat aaactgcgtg tggtcttcca
    18181 cattgcttgc atcgagtaac atagcgaaca ggatttgaat taactggtaa ttttgctagt
    18241 tttgcctgac tttccattat tacctggaaa tcatcagatg atttaatcaa ttgttttaac
    18301 tcgtttcggc gttgtctgta cttttctacc agcttcgcac gctttgactc tcgcatcagc
    18361 attgattttt tagccacaat ttcacccttt tattttctat ctttgtcttt cagtggaaga
    18421 ttaaatgctt ctaacaaagc tttagcttct tcatttgttt tagcacttgt tgtaatacaa
    18481 atatctaaac cgcgtattcc gtctgtttta tcataatcaa tctctggaaa cacgatttgc
    18541 tcatgtattc ccatgctgta gtttcctgtt ccatcaaaag attttggatt caaaccacga
    18601 aaatctctta cacgaggtaa agtaatagat attaatctat ctagaaactc atacatacgt
    18661 tcacgtctga gcgttacctt acaaccaatt ggccatccct ctcttatttt aaaaccagct
    18721 attgattttc tagcttttgt aacgacgggt ttctgaccag aaatcagcgt catgtcttca
    18781 atcgcatgat tcatgacttt tttgtcacca acagcttctc caactcccat attcagagtt
    18841 attttgagaa tcctgggtac ttccatcaca cttgaatagt tgaaccgttt catcatcata
    18901 gtgacgacgt ctttcttata aaattcttta agtctcgcca tttcaatcac ctaactctat
    18961 atacggtcaa ttatttcatt gttagactta taatatctaa cctttttgct aacaccatta
    19021 ctctcaagat atttaaagcc gaccttatca gccttttttg taacaggatt atagtgagca
    19081 acatttgaaa cgtgtaatgg agcttcacgc gctataatac cacccttatt ttcaggcttt
    19141 tgtggattag gcttgatatg ctttttaatt agatttccgc cttctactac aacggcatca
    19201 tcaattaccc ttaaaacctt acctatatga cctttacttt taccggcaat tacaattact
    19261 tcatcgcctg atttaatacg tttcatatct aatccttctt acaatacctc tgcggcaaga
    19321 gaaattatct tcataaatct ttctcttaac tcgcgtgtaa ccgggccaaa tatacgtgtt
    19381 cctattggct cgttttggtt gtttaaaaga actgctgcat tatcatcaaa tcgaatcaat
    19441 gaaccatcat ctcttcgaac accttgagca gttctaacga ctaccgctct cattacagca
    19501 ccctttttta ccttacttct tggtatagca tcttttacgc ttactttaat tacatcacct
    19561 acacgggcat accgtctgtg tgatccacca agtactttaa tacacattac tttacgtgct
    19621 ccgctgttat cagctacttc aagcactgtc tgcatttgga tcatttttct ctccagatct
    19681 aatttcgacc agaaaaaggc tgctgattat atcaggcctt ttagggtttt aaaagtaaaa
    19741 caattaagtt acttatgaaa ttacttcgac taatacccag cttttcgttt tagagagtgg
    19801 tcgtgactca cggattttta cggtattacc aactcggcaa acttgatttt catcatgtgc
    19861 gtgtagttta gttcttcgct tcatgatttt cccatacttt ggatgtttca cagtacgttc
    19921 aatcataact acaatagttt tatccatttt gtcgctaact acttttccaa tcattgttct
    19981 ggcatttgat tcactattag tagacatttc acttacctgc cttttcagtc aagattgttt
    20041 taacttttgc cactgactta cgcaccatag tgatcagatg tgttttatct agtgagccac
    20101 ttgctttttt cattcgtaaa ttaaattgat ctttacgtaa tgaaagcaat tcattttgca
    20161 attcctcaac tgacatattg cgcaattcat ctattttttt cattacatta ccgtccgctc
    20221 ttcaaacatc actttgaaag gaagttttgc ttttgcaagg tcaaatgctt ccattgcaag
    20281 ctctttgcta acgccttcca tttcaaataa aaccttacct ggttgtatct gggctaccca
    20341 atactcaaca cttcccttac ccttaccttg tcttacttca agtggttttt gtgtgattgg
    20401 tttatctgga aatactctaa tccaaatttt accaccacgc ttaatatgtc gtgtcatagc
    20461 ccttcgcgct gcttcgattt gtctagcagt taaacgtcca cgctctacag cctttagacc
    20521 aaactcaccg aaactaattt tactaccacg aagagccaaa cctctattgc gacccttcat
    20581 ttgttttcgg tacttagtac gctttggttg taacataatt tctaatcctc actcactggg
    20641 cgctttcagt tgatcttttc ttttgcggga gaatttcacc cttaaagatc cagactttta
    20701 cgccaataat tccgtaggta gtcttggatt cagcggtacc ataatcaata tcagctctaa
    20761 aagtatgtaa aggcactcgt ccctctctgt accattcact tcttgcaatt tcagcaccac
    20821 ccaatcggcc acttacacaa attttaatac cctttgctcc agctttcaga gcagaggtaa
    20881 cagcacgttt catagctcgt ctgaacatga cacgttgctc taactgctga gcaatacctt
    20941 cagccaccaa agtagcatcc aactcaggct ttttcacttc ttcaatattc aaatgaactg
    21001 gcacacccaa gttatcggaa atctctttac gaagtgtttc aattcctccg ccctttttgc
    21061 cgataataac acctggtctt gctgttagga tagttacaac agcgttatta gctggtcgct
    21121 caataagtat cttgctaaca gctgcagcca tcaatttctt tttaagatcg ttacgtagtt
    21181 ttatatcctg aattaaaaac tcagcatatc gctttcctgc aaaccatttt gagttccaat
    21241 ctttaattat tccaaggcgt atacctattg ggtttacttt ttgtcccatt gctattcctc
    21301 gtcagacact ttaatagtta tatggcaggt acgtttgcaa attcgatttg ctctaccctt
    21361 agctctggga ctaatacgtt ttaaagttgt tgcttcatca acgcatacca tgcctacttt
    21421 cagatcatca atatcagctc cattattatt ttcagcattt gctatagctg actctaataa
    21481 tttgagcatt aatttagcac ccttttttgg tgtaaacttg aggacatcaa gcgcaccaga
    21541 cacattcata ttacgtatca tatcggctac taatctaccc ttttgtgcgg ataaaggagc
    21601 gccttttaat ttagctgtta cttccatcat atcctcttac ttacctttag cctttctgtc
    21661 cccagagtgg cccttgaatg tacgagtcat ggcaaactca cctaatttat gaccaaccat
    21721 attgtctgta ataaatacag gaacgtggtc tttgccgtta tgcacagcaa ttgttaaatc
    21781 aatcatttca ggaacgattg tagaacgtct agaccaagtt ttaattggct tttttgattt
    21841 tgattcgatt gcagcttcta ccttgctgat caaatgatgg tcaataaatg gaccttttct
    21901 aattgaacga gccacttgat atcctcatat taattatttc ttcttacgtc ctctgacaat
    21961 aaatgtgtca gtacgcttat tgcttctagt tttataacct tttgttggca atccccatgg
    22021 agatacagga tgacgtccac ctgatgttcg tccttcacca ccaccatgtg gatgatcaac
    22081 tgggttcatg gcgaccccac gaacagtggg acgtatccct ctccatcgtt tggcaccagc
    22141 tttacccaat gctcttaggt tgtgttcgct attacttact tcaccaatta cagctcggca
    22201 taaaacatgt atcttgcgca tctctcctga acgtaatctt aaagttgcat aaacaccttc
    22261 ttttgcaaca agttggccac tgcaaccagc acttctgagc atttgcgcac ctttaccagg
    22321 cttcatttca acacaatgta ttgttgtacc tacaggtata ttttttagtg gcagacagtt
    22381 tcctacacta attggtgaat cagctccact ttgaatagta gctccaactt ccagatttga
    22441 tggtgctata atatagcgct tttcaccatc tttgtaggat atcagtgcaa ttagggcagt
    22501 tctatttgga tcatattcta atctctcaac acgacctaat ataccatcct tattgcgttt
    22561 aaagtctata attcgatatt tttgacgttg tccaccacca atatgtctaa cagttatacg
    22621 accttggttg tttcttccac ctgttttttt tagtttttca actaatgcag catgtggctt
    22681 acctttatag atatcgtgat gtacaacacg tatttcacct cgttttccgg gtgaagtcgg
    22741 tttagatttt aatagtgcca tctttatctg ccttattcag taactgtaaa gtcaatatct
    22801 tggtctgcat gaagagagac aaatgccttc ttccagtcac ttcgcttccc actggtttgt
    22861 ttgaagcgtt tagatttacc cttcacattg acaacagata cacttttaac ttttacatta
    22921 aatatatgtt caactgctaa tttaatttca gtcttagtcg catttttcag tactttgaaa
    22981 gtgaactgtt taaacttatc ggccatgaca gttgcctttt cagaagtatg tggttctcta
    23041 agaaccatca tcaatctctc agcgttcatt gtaactgttc ctcaattttt ttaattgcag
    23101 cttctgtaac aacaactttt tcaaatcgta gtaaggaaac aggatctata gttgtaacat
    23161 cacagatatc ataatcaatc aggtttcttg aaccaaggta ttcattttca ccaacttcac
    23221 tcattataat cagagcatta cttatttcca tctgattcat ttttttgagg aaatccttgg
    23281 ttttgtgatt atcacactgg aaatcactaa caaccaccaa gttcccagtg cggcaaagtt
    23341 cggagattat actacgtaat gcacgtttgt acattttttt attaagcttt tgtgaataat
    23401 ctcttttttt agatgcaaat gttactccac cacttctcca taatgggctg cgaatggttc
    23461 ctgctcgcgc acgacctgtt cctttttggt tccagggctt tttaccacca ccactaactt
    23521 cagaacgagt tttctgagcg ctattaccgc ttcgagcatt gttcatgaaa gtaactactg
    23581 cttgatgaac caagccttcg ttgtatgtat aagcaaatat ctctttattt agctttaatt
    23641 ttgactttgt atctattgta gtaatttcca tcactcacct cgtgcttgct ttttgactgc
    23701 aggcttaatt tctactctag aacctggagc accaggaatt gcacctttaa tgagcaataa
    23761 atttctttca gagtcaactt ttaccaattc cagactttga attgtacatc gagcattacc
    23821 catgtgaccg gccatttttt tccctttaaa aacacgacca ggggtttggt tttgtcctat
    23881 agaaccaggt actctatgtg atcttgagtt accatgcgat gcatcttgag ttctaaagtt
    23941 atgtcgtttt acagttcctg cgaaaccttt acctttagtt agacccgata catcaacata
    24001 ttgtccggca gtgaatacat cagcaacaga tatgacttga cccggggtaa atgcatcttc
    24061 tgaatctata cgaaattcta cttgcatatc accagcatcg atttgtgctt tagcaaaatg
    24121 cccagccact ggcttactca ctttgctaga tttctttgta ccaccagtta attgtacagc
    24181 tgaataacca tcattcgctg ctgtttttac ctgagaaacg cgattcggct gaacctcaac
    24241 aacagaaaca ggaacagaaa ctccttctgg tgtgaagaca cgcgtcatac cgatcttacg
    24301 gcctaataaa ccgatcatca ttgtaacacc tctttaatat cctttgcctt tatatttagt
    24361 catcaaggct tatctgaaca tcaaccccgg cagccaaatc caatttcatt aaagcatcta
    24421 cagttttttc agttggatga acaataacga ccaggcgttt atgagtacgt aattcatatt
    24481 gatctcttgc atctttattt acatgcggtg aggtcaatac agtaaatttt tcttttctga
    24541 taggcaaagg aattggtcca cgtatttgtg cgccagttct tttcgcagta tcaacgattt
    24601 ctcgtgttga tagatcaatc aaccgatgat caaaggattt taatcttatt ttaatatttt
    24661 gattgctact cattttt
//
LOCUS       DACTJE010000030        24539 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.030, whole genome shotgun sequence.
ACCESSION   DACTJE010000030 DACTJE010000000
VERSION     DACTJE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24539
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.030"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            156..2162
                     /gene="tkt"
                     /locus_tag="I9760_000953"
     CDS             156..2162
                     /gene="tkt"
                     /locus_tag="I9760_000953"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAT5042731.1"
                     /translation="MNSFTELANAVRMLSIDAVNQAQSGHPGMPLGMADIATVLWKKF
                     LKFNPKNPHWFNRDRFVLSNGHGSMLLYSLLHLTGYNLDISELKNFRQLHSKTPGHPE
                     HGHTPGVETTTGPLGQGLANSVGMALAERVLASTFNHDHFNLVDHYTYTFAGDGCLME
                     GISHEACSLAGTLGLGKLIVFYDDNGISIDGKVESWFTDDTTSRFKAYNWQVIGPIDG
                     HDADQIEQAISAARENTTQPSLIICKTVIGLGSSVAGSEKAHGSPLSAQDINNVREFF
                     NWKHAPFEIPDSIYKQWDHREQGEKEEQQWLQLLHEYRQQHPSEHDEFLRRANGDLPD
                     DWQEISAGFLKQCLNNEKAIATRKASQQCIEFFAPILPEMFGGSADLTGSNNTDWSGS
                     KAITAHNFSGNYLYYGVREFAMAAIMNGLAVHGGFIPYGGTFLVFADYARNAIRLSAL
                     MKQRVIYVLTHDSIGLGEDGPTHQPIEHIAMLRMTPGMTVWRPADLMETAVAWQQSLE
                     HHNGPSALLLSRQNLPALAHGTDAADLIKKGGYIIADCEGKPDAILIATGSEVQLAIT
                     AAEKAKARGLQVRVVSMPCAERFLAQSDDYKNQVLPGQVRTRIAIEAASSAYWHQFVG
                     FDGAVIGLDCFGVSAPATDAFNYLGITVEKIINTLDTLTKQTMV"
     gene            2244..3236
                     /gene="gap"
                     /locus_tag="I9760_000954"
     CDS             2244..3236
                     /gene="gap"
                     /locus_tag="I9760_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAT5042732.1"
                     /translation="MTIRVAINGYGRIGRCILRSIFEYNRQNEFEVVAINDLSGIDVT
                     AHLTRYDSTHGPFRSDVSIEGNMLIVDGHGIQVIAERDPAKLPWKQLDIDIVFECTGF
                     FTSRDAAMGHINAGAKKVLISAPAKGVDTTVVYGVNHSVIKASDVIVSNASCTTNCLA
                     PVVKVLNDAIGIESGLVNTVHAYTKDQMLLDGSHKDLRRARSATQSIIPTKTGAAAAV
                     GLVLPELAGKLDGFAMRVPVLNVSVVDLTFQAKRATSVEEINDIMRKAKNRILQINED
                     PLVSCDFNHNPASAIFDTTETKVIGNLVKVVAWYDNEWGFSNRMIDTAHQMMNC"
     gene            3247..4437
                     /locus_tag="I9760_000955"
     CDS             3247..4437
                     /locus_tag="I9760_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAT5042733.1"
                     /translation="MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTI
                     KSALDSGAAVIVLSHLGRPEEGKYEKKFSLEPVADYLRENLEYPVRFVKDYLSGVGVN
                     PGELVVCENVRFNPGEKANDEALAKKLASLCDVFVMDAFGTAHRAQASTYGVAQYAPV
                     AVAGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIA
                     NTFLKAQGFEIGISLYEPDLLDEARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFN
                     KSLSNVAADDMILDIGPETIRDYVDLIHDANTIIWNGPVGVFEFPQFAYGTRAIAIAI
                     AESDAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKH
                     VKTN"
     gene            4421..5845
                     /gene="pyk"
                     /locus_tag="I9760_000956"
     CDS             4421..5845
                     /gene="pyk"
                     /locus_tag="I9760_000956"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAT5042734.1"
                     /translation="MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSAL
                     QLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNKSITLIDGQNFTLDCMAPDTLGD
                     INGVSVAYPNLANELSIGDHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNR
                     KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPII
                     AKIERMEALDHLTDIIREADAIMVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVIT
                     ATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLFPVKVITMVNKICLS
                     AEKHASFFYHSDPETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWVSRHH
                     STVPIFAISANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELIKSGHLEKRGY
                     VLLTRGTQIGMPGGTNCMEIIPVV"
     gene            5936..11563
                     /gene="sdhB"
                     /locus_tag="I9760_000957"
     CDS             5936..11563
                     /gene="sdhB"
                     /locus_tag="I9760_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdhB"
                     /protein_id="HAT5042735.1"
                     /translation="MLLLSIEVINMPDHNSSDIPLSQQIDEVRGLLLKALKAQLDDLI
                     AGKLTPQPSGVPFVHFEKDPPQIATLKKVINCLYHAEEAFKSWESIDTSTLLGKAKAA
                     PKLIRALTQIYKSLGLLDEATPEIRSVIADNYHLLEPLFTQAYTIVQESGWLSEFMEM
                     DVTDKASKVIFQGMDLLGPDIDKWHDTHPLVDAFAKISKLVETISKLQEKDMPGKEKE
                     QAVELVRALLDDLDNNPFISKLSISDFEDSKAIKDLLEWFKNIQDDGFDFSKKSIKQY
                     VSWANHYLPTLITFADQLERQNYLKPGSLSTNLCSQIEHLGKQVNGILSEPNFGITER
                     VVSIESLKGKREQQIHNAQANSVRAIMATEKQQAATADFYRILEAYKGMNLSQVTEPD
                     RILLRQLYPQIQTALAHASLDLENLLTSVLNTTGPEKTPAKPKSWWELAKGGLGYVAS
                     YVVNYNVDKILGTRESVDRFLSSQIASEQFKILIAEKAREKLRTGSDKLEETALQYKA
                     EVRVNAIKSTLRKQPLQREAPPSTFVAVKPSKLVNLRGTLTAIQEMQLSTTVNSTRTN
                     VDTLIHRHLPEKYQACFSTLPYQVNEKDPELVKQIKTIENNLFQLEKALKDFEKIDLS
                     YGIMIRLHYFIAIASAASKLKSSVKELSPEGQKALAPLLQQIMTLGSSFSDTNYTTND
                     LSALQQLKTEGQARPGSPLGTSPEKTKTLPEETIKGNDKKPKEKTTELLDYADKISTA
                     RTLLLSKFKSTLSPAIADSLNPQEHGVPFIHLDQDPPQTAAIKKIINSMYHAECALKT
                     WHGIDTSTRLGKIAAAHQGVAALSQVYKSFALFTEDFSDIHNLIRENHDLIAPVIIEA
                     NKLVKEYGWASQFSELDVAQKLGSILGQGINTVQPKTDEITQTASLVKLFSEIPRLLN
                     NISSSLDENAEKSVDELRINSKKIEAIGAVFELFFEENAYLMSMFKGFSAISGLIELN
                     KKIQREGNNLQEITIRQYQKWLKEGYPNLMMMLDEIETRHYLTPGLLSAPIALELDQI
                     NDKLNEIIETKPDFKLQKIPLSFYMGDKRIERLMTKKTEHCLALLQIEEQKKAVNTFF
                     GILKHYSGQPLSNIKPNELAQLRLAFVKIQSAMANSNLELSNDFVAALNQLETSPSEE
                     INKIKVTVSQLIKLQPAVENNLSEQKKSSELKIAVIDKAIEHVIAKSFELKSDVSKEI
                     NIPQLRRTFLETESKKPIVGPGELKPTSVSSLNTVRGNLAYIQELKLSSTVGMIREQF
                     SALTRDNFSTRMQKYLIKPPGKPLHVINDKDPLMVRQIKEVENSLYHLETALKQFEQM
                     KKSDSFVSQTKALVEIANHAHELKMAVERFSPELKEHYGPVVRTALSFSNKVQSINYN
                     KEDWADLQFILNRAHKELLKRKTPRDHRIEKAFFEAGIREVQIDRTESAGKKAAKLGV
                     KYAHLASPQLEKARAYLSSRYKNAFGQQPKAIRSLTREQLANEEFMDAEISRLKKTLE
                     EKYGFNIATIKVLLALLEQIQRAGAQAAEVAGMVNTLVTYDFPKIKENAYRDLLTKLS
                     QEEDYLNLKPGTLINPAMAAVNQLFLSAALELDMPFSKKLSILDDTTYVNILVRQTES
                     DLQTLRKKLSEDPSNHEIAFEIEIKNDKLAFLRQQMVLLEDKNPNQTRNVLLDMQFEV
                     SLRNHLVYTTLKAPIAQEYEQIAREYYKKNKNRFLYADDCSKELLQGLKEFEKKHLPD
                     HLIVFEAYDRLRLFSAKLPPKNQDVKEYIENINRELQNRDIPIEQRAIKVKSLPNDAV
                     FIEKLSSADKGTHFLRKFIQFITIITASITEAIKTGGSLIYIYRQKQMEQSIKNIEKS
                     ISLKEELHTIKNTDGASPRKGEGQEGEIEFTKL"
     gene            complement(11663..12733)
                     /locus_tag="I9760_000958"
     CDS             complement(11663..12733)
                     /locus_tag="I9760_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042736.1"
                     /translation="MRIATLFLAVLFISPLWAAPKFFPSQPALNGVYDIKGTELCSTA
                     KHTLAYLNKGKNYDPQVIHDGKVIKISLDRVKKTLEFICQNQKKLNNPDFVKQHFDFI
                     RWYPDLSQAKQLSANKPLLQNIPKDKILMTKYFVHLAGASTKPSSAKPYPLYALPEDE
                     QNLTLDKANAKPGLIRFRYGKQAILSGALKNQPVSELAYLSRDDLEAALLQGTVVADF
                     GPTAGKRIFNVHRSNNIAYDRTKTPYQQERFWYFKEVDGIKGYGKDAEYKITVNPKVT
                     FAADLAEFGLGKLLMIQYQDKSGRTMTKMGILADTGGAFNNNLYQIDYLAGSFAGKDV
                     LYRATRDLPDYVDAYFMILKQP"
     gene            complement(12734..13426)
                     /locus_tag="I9760_000959"
     CDS             complement(12734..13426)
                     /locus_tag="I9760_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="activator of prop osmoprotectant transporter"
                     /protein_id="HAT5042737.1"
                     /translation="MRKQALHPRTAVINKAQKNQSKRARSDALLWLAANFPEAFDNSL
                     RIRPLKIGIMSDILQHAEKAEQVGISKSKLREAVVLFTRRLDYLACLKAREVRIDLHG
                     NPVAEVTEEEAEHASMKIKKRVEKSVKNARKQVNAKAANHSHVNSQPSAVSSVKPMNS
                     FDSHPEPLLPIYPLRSSTYASQNVAMQSAKSPSVVVKHKAPKQYDPDAVARLKEKLGL
                     SRKAEEKKETTE"
     gene            13783..13968
                     /locus_tag="I9760_000960"
     CDS             13783..13968
                     /locus_tag="I9760_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042738.1"
                     /translation="MRIILSILAIFIPWLVLLIKDNPGGAVVALIMQATLIGWPFASI
                     WAWRAVNPLTPTSQSKQ"
     gene            14148..14879
                     /locus_tag="I9760_000961"
     CDS             14148..14879
                     /locus_tag="I9760_000961"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAT5042739.1"
                     /translation="MRTRIIVNGANGKMGILACETLENHEQFEIVAKLSRQDNLGQSI
                     LDTKAQIVVDLTRADCVYENSLTIINHGARPVIGTSGLVETQIDELTKLCEIKQIGGI
                     IAPNFSLGAILMMILATKASEYFSEVEIIEGHHQQKLDAPSGTALKTAEMIAAARKKP
                     KNKLPLKELTPGARGGSHHDINIHSLRLPGLLARQEVLFGNIGETLSITHNSIDRRCF
                     MPGIVLACQKVLNLNNLVYGLEHLL"
     gene            complement(14983..17451)
                     /locus_tag="I9760_000962"
     CDS             complement(14983..17451)
                     /locus_tag="I9760_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT5042740.1"
                     /translation="MGKGIILRVLENTILSPQVFDTLERLLPGYKVEYFKEQPDYRKS
                     IARRIDSLHDAFSFILKAYPLDPKHTSLTVATLSTYAAECKASCDLEKLTLEELHLEL
                     ERFTAKLVEAIAIAWKWPKGKAVKEAIASLNEAEQYVLMSRGRSDIATIMPIEMGSET
                     KYVLQYDESLSPVYEQWLSELKQLKEYNFPKTPAWFKNLPPYQQAYYCNLNLSSVDPK
                     KALQHFNTFFGNWGDIAKRSLNLTTELNQIHTNSPPYPSWFNELSPAQQAMIRVLSAT
                     PHEIKSSLKEFKKFMVEQARNDQYASTLSLVPKLPQWYWVLSEKQQYFLEYALKNAEK
                     IEDVVSYLSSRHRTLPAPGNYGAHSLYLIDDEGKETLFYDKRYRSSHVASRDSLKFPE
                     DVQQRHVDSNLVKVMEFAKPQQPLLLQTLISPIHAVDYIPTVVTDFLPELPPDLELYK
                     IAREAVTRSKRRHEIFQHNHPFNIAKRYYYTQATDTDSEFLLKAAQKYASSKPGLQAL
                     IDDYKAVLESPLGSATFWDYDGRELFLSSLEELIILNMGGYSYGSCVSGKDRKAVELL
                     HTDAMILYKAKYGNWPKFGIPKEKQERVNFINIVVDLYISRHQHELAGQNAPGSEGIK
                     TPDWYWPNDIAEAINERLGTEKALAYDDRLATDNEVKNISKDLRSFFLPENELHCLLI
                     AKQLGEKMCTMLYDVLSALINEERRFQKSSKDSWKLRWFSDKDVSSTPTGILNIREVM
                     HDENSGNDNVLRIGKIFAAVLNRPESDSSRTTATNSVYDRIRKLLQPLSSETTLQTLA
                     EEAILEWSSLFESSKRENSGLVYV"
     gene            17629..17805
                     /locus_tag="I9760_000963"
     CDS             17629..17805
                     /locus_tag="I9760_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HAT5042741.1"
                     /translation="MKSIEDFTGFSESGIDDAIQNALKKAGNPTHFEVVETLGSQDSQ
                     SDRRYQVTLKTLSD"
     gene            17916..19415
                     /locus_tag="I9760_000964"
     CDS             17916..19415
                     /locus_tag="I9760_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAT5042742.1"
                     /translation="MGYNVPHYIGGQLINETDTEAQAIYNPALGEIIGQVHFASKATC
                     DRAVAIAKAAGNEWSQTPAIKRARVLFRFRELLEKYQMDLARIVTREHGKTLDDAKGS
                     VARGIEVVELHCGLVNELKGDFSAEVASHIDCHTFRQPLGVCAGVSPFNFPVMVPIWM
                     MIPAIACGNTFILKPSEQDPSAPVRLLELLSDAGLPPGVANCIQGNKTTVEHLLAHPD
                     IAAFTAVASTPVAEYIYTTAAAYGKRAHTFGGAKNHCVVMPDADLDQAANAIVGAAYG
                     SAGERCMALSVVVTVGQHTADALINKISPLIRAIRVDAGDVEGTDMGPLVSQAHRQRV
                     LAAIEKGVNEGARLIIDGRTFQHPQYPQGFYLGPCLFDEVTEEMSVYQNEIFGPVLVI
                     VRVNHFEEALALVNKHQYGNGTAIFTRDGFTAREYSQRVQAGLVGINIPIPVPIANHP
                     FGGWKRSVFGDTNMHGEESIHFYTRRKTVTSKWPITELKDSSFAMPTHD"
     gene            19439..20335
                     /gene="mmsB"
                     /locus_tag="I9760_000965"
     CDS             19439..20335
                     /gene="mmsB"
                     /locus_tag="I9760_000965"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HAT5042743.1"
                     /translation="MANIGFVGLGHMGFPMAINLLKAGHSVMGYDLQQSALEHFAEQG
                     GTIAHNLQELAKGKDVIVTMLQTGQQVLHVCLGVDGIFSQLNAGSLYIDCSTIDVKSS
                     RETHQIAKENHLLVVDAPVSGGVAGATAATLTFMVGGEAEAYNAAHPILSAMGKKIIH
                     TGGAGSGQAAKICNNMILGTTMIAISEAFILAKELGLSDGKLFEVVNNSSGQCWAMSK
                     YVPVPGILENVPANNDYKPGFAANMMLKDLLLSQNTAKSVHIETPLGAKTTEIYQQFV
                     DQGLGDIDFSAIIKHIGKIQEV"
     gene            20337..22220
                     /gene="acs"
                     /locus_tag="I9760_000966"
     CDS             20337..22220
                     /gene="acs"
                     /locus_tag="I9760_000966"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAT5042744.1"
                     /translation="MLPVHLKHPHLTATEEYLSEFWSEIAAQTIDWIQPWSITLQGGL
                     HKGDVKWFQGGLLNVSANCLDRHLPHKANQTAIIWEGDDENQNKTLTFAQLYSEVCKM
                     SNVLKSLNVRRGDTVGIYLPMIPEAAIAMLACARIGAIHTVVFAGFSAHALQQRLIAS
                     SCKCLITADAFQRGGKTIPLKKQADEASVDLNITKLVVKNSNAPTALNKNKEHWWHEL
                     KQTVSDQCTPEPMNTEDPLFILYTSGSTGQPKGVVHTTGGYLVQAAYTHQLIFACQDN
                     EVFWCTADVGWITGHSYVVYGPLCNGITTLMFEGIPTWPDAARNWRIIDKHQVNVFYT
                     APTAIRSLMRAGDQWLNSSSRSSLRLLGSVGEPINPEAWNWYHQKVGQGKCPIVDTWW
                     QTETGAIMISPRASDEVIKPGSARKPIPGIVPLLLNEQGHEINGAGEGLLAIKYPWPS
                     MARTIAGDHQRYCNTYLSNGYYITGDGAKRDEDGDYWITGRIDDVLNVSGHRLGTAEI
                     ESALVSHPKVAEAGVVGIPHDLKGQAIFAYVILKQGNKPDAELQTELMERVKDQISAI
                     AKPDVIQFANDLPKTRSGKIMRRILRKIACKEVSHIDELGDLTTLANPQIVEELMKNI
                     LKR"
     gene            complement(22317..>24539)
                     /gene="cegC2"
                     /locus_tag="I9760_000967"
     CDS             complement(22317..>24539)
                     /gene="cegC2"
                     /locus_tag="I9760_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC2"
                     /protein_id="HAT5042745.1"
                     /translation="ETSQELLGEVLTELALSNPVEENKLSPKAGINFNGEAFLHLIVK
                     SAIQQKINQLAYLPEKAKQEIESGLINFYFGGASSSLTRASGLRALFIANIFSKKSIE
                     ALFPPKALFAKAIANYLTLKTLPDLLIEYLKSKDTSPIDEEFFDVVLASATFKDPDEF
                     FENLFRLSRINPEVAKALFVFASQKINVLFGISLEEYAKKIALKDSGEFKSWFESLSK
                     PQPVIKIPEIDNVLRQQRVDDAKRVISDIVQRINSFPFSFEGFKTVEHVNLNAEELRG
                     QLKKIVHSGELQNALQILDLPDGHPEVQRALERKLRMIDAAANRRSDFLRKYETDIDE
                     HVRQIKNFPIDFNDADTIVAIESQRILLNKKLHTLVKAEDLLGEQFIANPKIKMVYYA
                     QVEKINLRAELLQKRLLDEAQKVIDSVEKRIDNFVIRFNDISSTSAVEWQRNNLLQQL
                     DNLVKPNQALLSSEKVLDCNNLQPSIVRALQAKKQEINETADQLIIKINAEEVVKSYE
                     KQIREFPISFSRCQTVEEVIARKQDLIQSVRYLVDNKPDLLKAREQLQLSDEYHSDIK
                     IALTDKICEINRQADVMSKRITDQIAAIKETLNILAEIKFSDHLKTIESMVKTLETKA
                     VGDENYKRAAPIARTFYNNLLRAEEHFKNSQLPKNVKCNDFHQACVRAINAVMPVLEV
                     HRGWKQVFADLASALVTLCTLGGANLYAGRWRLFPVPTESEKIVKDFSLSMQPLSVSA
                     "
BASE COUNT         7518 a         4996 c         5004 g         7021 t
ORIGIN      
        1 ctccatcatt atgtcaaaaa ttaggaaaaa tttaaactta aatttacatt ttgctctaat
       61 attgtcatta ttttcaacta aaaatgaatg gtcttcttgc cgatttatgc cttctgagcg
      121 acaatagtgc ctcagttaaa tgcaaaagag taggcatgaa cagttttaca gaattagcta
      181 atgcagtacg catgttaagc atcgatgcag taaaccaagc tcaatcaggc caccctggca
      241 tgcccttggg tatggctgat attgccaccg ttttatggaa aaaatttcta aaattcaacc
      301 ccaaaaaccc gcactggttt aacagagacc gttttgtatt atctaatggc catggttcca
      361 tgcttctcta ttctttactg catttgaccg gatacaactt ggatattagc gagcttaaaa
      421 acttccgcca attgcactca aaaacaccag ggcatccgga acatgggcac actcctggtg
      481 tcgaaacaac aacagggcca ttaggtcagg gtttagccaa ttcagtcggg atggccttag
      541 cggagcgtgt gctggctagt acctttaacc acgatcactt taacctggtt gatcattata
      601 catatacttt tgccggtgat ggttgcctga tggaaggcat ttctcatgag gcgtgttctt
      661 tagctggcac tcttggtttg ggaaagctca ttgtatttta tgacgacaat gggatatcta
      721 ttgatggtaa ggtagaatcc tggtttacgg acgatactac gtctcgcttt aaagcctaca
      781 actggcaagt cataggcccc atcgatggcc acgacgcaga tcaaattgag caagccatat
      841 cagcagcacg cgagaacacg actcaacctt cattaattat ctgtaaaacc gtcataggac
      901 tgggctcctc tgtagccggt agcgaaaaag cgcacggctc tccattgagt gcccaagaca
      961 taaataatgt ccgtgaattt tttaattgga aacatgctcc ctttgaaatt ccagattcga
     1021 tatacaagca atgggatcat agagagcaag gagaaaaaga agagcaacaa tggctgcagc
     1081 tcctgcatga ataccggcaa caacatccat cggaacacga tgagtttttg cgtcgtgcca
     1141 atggcgacct gcccgatgat tggcaagaaa tatcggctgg attcctaaaa caatgcctga
     1201 acaatgagaa agccatagca acgcgtaagg cgtcgcagca atgcatagag tttttcgcgc
     1261 caatattacc ggaaatgttt ggcggctcag cggatttgac tggttccaat aataccgact
     1321 ggtcaggcag caaagccatc acggcccata atttttccgg aaattacctt tattatggag
     1381 tgagggaatt tgccatggca gccatcatga atggacttgc tgtgcatggg ggattcattc
     1441 cctacggcgg aacattcctt gttttcgcag attatgcaag aaatgccatc cgtttaagcg
     1501 ctttaatgaa acaaagagtg atatacgtat tgactcatga ttctatcggt ttaggggaag
     1561 atggcccgac acaccaacct atagaacata tcgctatgtt aagaatgacc cctggcatga
     1621 cggtctggcg ccctgctgat ttgatggaaa ccgctgttgc atggcaacaa tcgttagaac
     1681 atcataatgg cccgtctgcc ttattactct caagacaaaa tctgcccgct ttagcccatg
     1741 gcaccgatgc ggctgacttg attaagaaag gcggatatat tattgccgat tgtgaaggga
     1801 aacctgatgc tatcctgata gccactggtt cagaagttca actggctata accgcagcag
     1861 aaaaggcaaa agcaagaggt ctccaggtca gggtggtatc catgccctgt gctgagcgat
     1921 tccttgccca gagtgatgat tacaaaaacc aggtattacc tggtcaggtt cgcacacgta
     1981 ttgcgataga agcagctagc agtgcctatt ggcaccaatt tgttggcttt gacggcgctg
     2041 tcattgggct ggattgtttt ggggtatcag ctcctgctac agatgctttt aattaccttg
     2101 gaattacagt agaaaaaatt atcaatactc tagatacatt aaccaaacaa acaatggtgt
     2161 aacccatacc ccttctgaag tttcaagaag gcctgtattc agtataactg agtatggagg
     2221 ttaccctaac tcggagaagt agtatgacaa tacgagtcgc gattaatggc tatggccgca
     2281 ttggccgttg cattttaaga tcgatttttg aatacaacag gcaaaatgag tttgaagtcg
     2341 ttgccattaa cgatttatct ggcatcgatg tgaccgctca cctgactcgc tacgattcaa
     2401 ctcacggtcc ttttcgctcg gatgtgagca tagaaggaaa catgctgatt gttgatgggc
     2461 atggcattca agtgattgct gagcgcgatc cggccaaact gccatggaaa caattggaca
     2521 ttgatatcgt ctttgaatgc accggtttct ttaccagcag agatgcggca atgggtcata
     2581 tcaatgccgg cgccaaaaaa gtattaatat ctgccccggc caaaggcgtt gatacgacag
     2641 tggtctatgg agttaatcac tccgtcatca aagcgtctga tgtcattgtg tctaacgctt
     2701 cctgtacaac aaactgcctg gcgccagtag ttaaagtgtt aaatgatgcc ataggaattg
     2761 aatcaggatt agtcaacact gtccatgctt atacaaaaga tcaaatgctt ttggatggaa
     2821 gccataaaga tttacggcgc gcccgctctg caacgcagtc catcattcca accaaaaccg
     2881 gcgctgccgc agccgttgga ttggttttac ctgaactggc agggaaattg gacggatttg
     2941 cgatgcgagt accggtatta aacgtatccg ttgttgattt aactttccag gcaaaacgcg
     3001 caacttctgt ggaagaaatc aatgacatca tgcgcaaggc taaaaaccga attttacaaa
     3061 ttaatgagga ccctttagtt tcttgtgact tcaatcacaa tcccgcttcc gctattttcg
     3121 acacaacgga aaccaaagtg ataggcaatt tggttaaagt agtagcctgg tatgacaatg
     3181 aatggggttt ctctaaccgc atgattgata ccgcacacca aatgatgaac tgctaaagga
     3241 taaccaatga acctgattaa aatgagcgac attgatctgt ctggcaaaag agttctgatt
     3301 cgtgaggatt taaatgtccc gatcaaagac ggtatgatta ccagtgatca aagattgcaa
     3361 gcggccctgc ctacaatcaa atccgctttg gacagtggcg cagcagtaat cgtattgtct
     3421 catcttgggc gtccggagga aggcaaatac gaaaaaaaat tctctttgga gcctgttgct
     3481 gattatttaa gggaaaacct ggaatatcct gttcgcttcg tcaaagatta cctgagcggc
     3541 gtgggtgtaa accctgggga attggtcgta tgcgaaaatg tccgatttaa tcccggtgaa
     3601 aaagccaatg acgaagcttt ggcaaaaaaa ctggcaagcc tttgtgatgt ctttgtcatg
     3661 gatgcttttg gcacggcaca cagggcacaa gcgtctacct atggtgtcgc tcaatatgct
     3721 ccagttgccg ttgctggtcc tctgctaatc cgtgaacttg aagcgttaaa ccaagtgctt
     3781 aaagcgccta aaaaacctat cgtcgctatt gttggaggag ccaaagtctc atccaagctc
     3841 agtctattga aacaattggt gggtatggtt gatgttctta tccctggcgg cggtattgcc
     3901 aatacttttc tgaaagccca agggtttgaa attggtattt ccctttacga acctgattta
     3961 ttggatgagg ctcgccatat ccttattctg gccaaagaga aaggttgcca aattcctcta
     4021 cccaccgacg ttgtagtagg caaaacattt agtgaaacct gccctgcttt caacaaatct
     4081 ctgtctaatg tggctgcgga cgatatgatc cttgatattg gcccagaaac cataagagat
     4141 tatgtagacc tcatccatga cgccaacacc atcatctgga atggtccggt tggtgtattc
     4201 gaattcccgc agtttgctta tggaactaga gccattgcta tagcgattgc cgagagtgat
     4261 gccttttcta ttgctggtgg tggtgatacc ttggctgctg ttgatcttta tgatttgaat
     4321 caacaaattt cttatatttc cacaggaggt ggggcatttc tcgaatgtct ggaaggaaaa
     4381 accttgcctg ctgttgctat tttgcaggag cgcgcaaaac atgttaagac gaactaagat
     4441 tgttgccact ttaggcccag ccagcaagga acctgaaatt ctgcgatcca tgctggctgc
     4501 tggagtcaac gtagtacgaa ttaatttttc tcatgccgat tcctccgccc tgcaattaat
     4561 tgcactggtt cgtaaaatcg cagacgaatt aaaccacccg gttgcagtca tggctgactt
     4621 acaagggcct aaaataagag tcggccgttt tcaaaacaaa tccatcactt taattgatgg
     4681 acaaaatttt acattggatt gtatggcccc agatactctg ggagacataa atggcgtatc
     4741 ggtcgcttat cctaatttgg ccaatgaact cagtattggt gatcatcttc tgattaatga
     4801 tgggttaatt gaattggaag tcatagagat ttccgggtca aaaattcatt gcaaggtagt
     4861 ggaaggtgga gtactcactg atttgaaagg tttgaatcga aaaggtggcg ggttagcagc
     4921 cagaacttta acagaaaaag atagaaatga tttacgtaca gcaattgaag cagaagtgga
     4981 ctacatcagt ttatcttttg taaaagacgc agaggatatc cggcaagcaa gagcgctaat
     5041 gaaagactat ggggcccaaa taacccctat tatcgctaaa attgagcgca tggaagcttt
     5101 ggatcactta actgatatta ttcgcgaagc agacgccatc atggtggcca gaggagattt
     5161 aggcgttgaa gtgggtgctg ctgaagttcc cgccattcaa aaacacatca tagaacaaac
     5221 gcgactttta gataaagtgg tgattaccgc aacacaaatg atggaatcca tgataagcaa
     5281 tcctcagcct acccgtgccg aggtttctga tgtagccaac gcgattctgg acggaactga
     5341 tgctgtgatg ctttcggctg aaacagcaag cggccttttt ccagtcaaag tcattaccat
     5401 ggtcaataaa atatgtttaa gcgctgagaa gcatgccagc tttttttatc atagtgatcc
     5461 ggaaacctgt cattatcaac gggctgacca ggcaatcgct atggctacca tgcataccgc
     5521 caatcatttc cccattcagg ccattatcac actgaccgaa tccggagaca cggctttatg
     5581 ggtatcccga caccacagca ccgttcctat ttttgctata tcagccaata agagaaccat
     5641 tggtcgttta agccttgtaa acaatgtctt tcctatctac attgattttc atcaattcaa
     5701 ccccgaagga ttgaatcaac aaattttaca tgaattaatc aaatcagggc accttgaaaa
     5761 gagaggctat gtgttattaa ctcgcggaac ccaaattgga atgcctggcg gcaccaactg
     5821 catggaaatt atacctgttg tctgagttca agatatcctg ttaattaaag caaggttctt
     5881 tcataaaaga ccaaaatgat tattttaatg acactttaat caaattatca cctctatgct
     5941 actattaagc atagaggtga taaatatgcc agatcataac tcatcagata ttccgttatc
     6001 gcagcaaata gatgaagtca ggggattgct ccttaaagcc cttaaagcgc aattggatga
     6061 tctgatagct ggaaaactca caccacaacc aagtggcgtt ccttttgttc attttgagaa
     6121 ggatccacca caaattgcaa ctctaaaaaa agtcattaat tgtctttatc atgctgagga
     6181 agcatttaaa agctgggaaa gcattgatac cagtacgcta ttgggtaaag ccaaagcagc
     6241 acccaaatta atccgtgcac taacccagat atacaaatcc ctgggtttac ttgatgaggc
     6301 tacccctgaa ataaggagtg tcatagccga caattatcat ttacttgaac cgctgtttac
     6361 tcaagcttat accatcgtgc aagagtctgg ctggctatca gaattcatgg aaatggatgt
     6421 gaccgataaa gcatccaaag tgatcttcca agggatggat ttattgggtc ccgatatcga
     6481 taaatggcat gatacgcatc ctcttgttga cgcctttgca aaaatatcaa aactggtaga
     6541 aaccatatcc aaactacaag aaaaggacat gccaggaaaa gagaaagaac aagcggtaga
     6601 attggttcgc gccttacttg atgacctgga caataaccct tttatcagca aattaagtat
     6661 cagtgatttt gaagactcaa aagccataaa ggatttatta gaatggttta agaacataca
     6721 agacgatggt tttgatttta gcaagaaatc cattaagcaa tatgtttcct gggctaatca
     6781 ctatttaccc acattaatca ccttcgctga tcaactggaa cgacaaaact atttaaaacc
     6841 aggcagttta agtaccaatt tgtgttctca aatagagcat ttaggcaagc aagtgaatgg
     6901 catactatct gaacctaatt ttggtattac tgagcgcgtt gtatccatag aatcattgaa
     6961 ggggaaaaga gagcaacaaa ttcataatgc tcaggccaac agtgtgcgag ctatcatggc
     7021 taccgaaaaa cagcaagcgg ctactgctga tttttatcga atcctggaag cctataaggg
     7081 catgaatctt tcacaagtta cagagccgga tcggatatta ctaagacaat tatatcccca
     7141 aatacaaacg gcactggctc atgccagttt agatcttgaa aatctgttaa cctctgtttt
     7201 aaatactacc ggccctgaaa aaaccccagc caaacccaaa tcatggtggg aattagccaa
     7261 aggtggttta ggttatgtag ccagttatgt ggtcaattac aacgttgata aaatccttgg
     7321 aaccagggag tctgttgatc gttttctgtc aagccaaatt gcgtccgagc aatttaagat
     7381 cctgattgct gaaaaagctc gtgaaaaatt aaggacagga tcagataagc tagaagaaac
     7441 cgcactgcaa tataaagctg aagttcgtgt caatgctata aaatcaactc tgagaaaaca
     7501 acccttgcag agggaggctc ctccatccac atttgttgca gtgaaacctt cgaaattagt
     7561 caatcttcgt ggaacactaa ctgccattca ggaaatgcaa ttatcgacta cagtaaacag
     7621 cacaagaaca aatgtcgata ctctgattca tcgccatttg ccagaaaaat atcaagcctg
     7681 tttttcaact cttccgtatc aagtcaatga aaaggatcct gagttggtga aacaaattaa
     7741 aaccattgaa aataatttgt ttcaattaga aaaagcactt aaagattttg aaaaaatcga
     7801 tttgagctat ggaattatga tccgattaca ttattttatc gcaatcgctt ctgcagcttc
     7861 caaactaaaa tcaagtgtca aagaactttc accagagggt caaaaagcac tagctccact
     7921 gttgcaacaa attatgactt tagggtcatc tttctcagac accaattata caactaatga
     7981 cttatccgcc ttgcaacaat taaaaacaga aggccaagcc aggcccggaa gtccactcgg
     8041 aacatctccg gaaaaaacaa aaacattacc tgaagaaact ataaagggaa atgacaaaaa
     8101 accaaaagaa aaaacgactg aattattgga ttatgctgat aaaatcagta ctgctcggac
     8161 attgttatta agtaagttta aatccacctt atctcccgcc atagccgaca gtttaaaccc
     8221 acaggaacat ggcgttccgt ttattcatct tgatcaagac ccgcctcaaa cagcggcaat
     8281 aaaaaaaatc atcaacagta tgtatcatgc ggagtgtgct cttaaaacct ggcatggcat
     8341 agataccagc actagattag gtaagattgc tgcagcacat caaggcgtag cagcattatc
     8401 ccaagtgtac aaatcatttg cgttgtttac tgaggatttc tcagacattc acaatttaat
     8461 tcgtgagaat cacgatttaa tagcacctgt gatcatcgaa gcaaacaagc tggttaaaga
     8521 gtatggatgg gcaagtcaat tcagtgaact tgacgtcgcc caaaaactgg gaagcatttt
     8581 aggtcaggga attaatacag ttcaacctaa aacggatgaa ataacccaaa ccgcttcact
     8641 ggttaaatta ttttcagaaa ttccaaggct actgaacaat atttcaagtt cattggatga
     8701 aaacgctgag aaatcagttg atgagttaag aatcaacagc aaaaaaattg aagccattgg
     8761 cgcagtattt gaattgtttt ttgaagaaaa tgcctactta atgagcatgt ttaaaggatt
     8821 cagcgctatc tcaggtctta tcgaattaaa taaaaaaatt caacgcgaag gaaacaacct
     8881 gcaagaaatc actattcgac aataccagaa atggttaaaa gaaggttatc ccaatttgat
     8941 gatgatgctt gatgaaattg agacacgcca ctacttaacc ccaggcctgt tatcagcacc
     9001 tattgccctg gaattagacc aaattaacga caagcttaat gaaattattg aaaccaaacc
     9061 tgattttaaa ttacaaaaaa ttcctctttc attctatatg ggagataagc gcatagagcg
     9121 cttaatgact aaaaaaacgg aacactgcct cgcattgctg caaatagaag agcaaaaaaa
     9181 agcagtgaac acttttttcg ggatacttaa acactattca ggccaaccgc tgtcaaacat
     9241 aaagccaaat gagctcgctc aattgcgtct tgcttttgtt aagattcaat ccgcaatggc
     9301 gaatagtaat ttggaattaa gcaatgattt tgttgctgct ttaaatcaac ttgaaacgtc
     9361 cccatctgaa gaaataaata aaatcaaagt tacagtctct caattgataa aattgcaacc
     9421 tgctgttgaa aacaatttaa gcgaacagaa aaaatccagt gaattaaaaa ttgcagtcat
     9481 tgataaagcc atagagcacg ttatagccaa atctttcgag ttaaaatctg atgtatcaaa
     9541 agaaatcaat ataccccagc tgcgcagaac atttttagaa actgaaagca agaagcctat
     9601 tgtaggtcct ggggaattaa aaccaacatc ggtctcatct ctcaacaccg ttcgtggtaa
     9661 tttggcttac attcaagaat taaaattatc atccactgtg ggtatgatac gagagcaatt
     9721 ttcggctctt accagagata atttctccac tcgaatgcaa aaatatttaa tcaaacctcc
     9781 aggtaagcct ttgcatgtga taaatgacaa agatccgctc atggtaaggc aaattaaaga
     9841 agtcgaaaac agtttatatc acctggaaac cgctttaaaa caattcgagc aaatgaaaaa
     9901 gagcgacagc tttgtatcgc aaacaaaagc gttggtagaa attgcaaacc atgctcatga
     9961 attaaaaatg gcagtggaac gattttcacc cgagttaaaa gagcattatg gcccagtagt
    10021 tcgtaccgca ttgagcttca gcaataaagt tcaatccatc aattacaaca aagaggattg
    10081 ggctgatctt cagtttattc tgaatagagc ccataaagaa ttgttaaaaa gaaaaactcc
    10141 aagagaccat cgtattgaaa aagctttttt tgaagccggg attcgtgaag tacaaatcga
    10201 tcggactgaa tcagcaggaa aaaaagccgc aaaactgggt gtaaaatacg cgcatctggc
    10261 ctcccctcag ttagagaaag caagagccta tttaagttcc agatacaaaa atgcgtttgg
    10321 tcaacaacct aaggctattc gttcgttgac tcgagaacaa ttagcaaatg aagaatttat
    10381 ggacgctgaa attagcagat tgaaaaaaac attggaagag aagtatggtt ttaatattgc
    10441 aacgatcaaa gttcttcttg ctttattaga acaaattcaa cgggctggag ctcaggctgc
    10501 tgaggtagcc ggtatggtca ataccttggt tacttatgac ttccccaaga taaaagaaaa
    10561 tgcgtatagg gatttactga caaaattatc tcaggaagaa gactatctga atttaaaacc
    10621 aggcacatta attaatccag ccatggctgc agtcaaccaa ttattcttat ctgcagccct
    10681 ggaattggac atgcctttta gtaaaaaatt atctattctg gacgatacca cttatgtcaa
    10741 tattctcgtc agacaaaccg aaagcgatct gcaaacatta aggaaaaagc tatccgaaga
    10801 tccctcgaat catgaaatag catttgaaat tgagattaag aacgataaac tagctttcct
    10861 caggcaacag atggtgttgc ttgaagataa aaaccccaat caaaccagaa atgtcttatt
    10921 ggacatgcag ttcgaggtgt cattacgtaa tcaccttgtt tatacaacgc ttaaagcacc
    10981 cattgcccag gaatatgaac aaattgccag agaatattac aaaaaaaaca aaaatcgctt
    11041 tctgtatgcc gatgactgtt caaaagagtt gctgcaagga ttgaaagaat ttgaaaaaaa
    11101 acatttgcct gatcatttga tagtttttga agcctacgac aggcttcgcc tattcagtgc
    11161 aaaacttccc cctaaaaatc aggatgtcaa agagtatatt gagaacatca atagagaatt
    11221 acaaaataga gatatcccta tagagcaacg tgctataaaa gttaaatcat tacctaatga
    11281 cgctgtcttt attgaaaaat taagttcagc agataaaggc actcatttcc taagaaaatt
    11341 cattcagttc attacaatca ttacagcaag catcactgaa gcaataaaaa ccgggggcag
    11401 tttgatttat atttatcgcc aaaagcaaat ggaacaatca ataaagaata tagaaaaatc
    11461 gataagcctt aaagaagaac ttcataccat aaaaaatact gatggcgctt caccccgcaa
    11521 gggagaaggg caagaaggtg aaatcgaatt taccaaattg taagactcaa taagccatat
    11581 tggtaaaaaa tgattctata ctctggactt accccgtgaa cagtgtgtaa acagtaatgg
    11641 caaaaatatt gatgtgaaat gcctaaggtt gttttaaaat cataaagtaa gcatcgacat
    11701 aatcgggtaa atctcgagta gccctgtaca aaacgtcctt tcctgcaaag cttcctgcca
    11761 aataatcaat ttggtataaa ttattattaa aagccccccc agtatcagcc aggatgccca
    11821 ttttagtcat tgtgcgtcct gatttgtctt gatactgaat cattagtaat ttccctaaac
    11881 caaactcagc caaatcagct gcaaaggtca ccttggggtt aaccgtaatt ttgtattcag
    11941 cgtccttacc ataaccctta attccatcga cttccttgaa ataccaaaat cgctcctgct
    12001 gatagggagt tttagttcga tcataagcaa tattattact acgatggaca ttaaatattc
    12061 tcttgccagc agtcggtcca aaatccgcga caacggtacc ttgcagtaaa gccgcctcca
    12121 aatcatctcg acttaaataa gccaattcgc taaccggttg atttttaaga gcaccggata
    12181 atattgcttg cttaccatat ctgaaacgga ttagtcctgg tttggcattt gctttatcca
    12241 gagtcaaatt ctgctcgtct tcgggcaaag cgtacaaagg atagggtttc gcggagcttg
    12301 gtttggtact ggcaccagcc aaatgcacaa aatatttagt catcaatatc ttatctttgg
    12361 gtatattttg taataatggt ttattggcgg ataattgttt ggcttggctt aagtcaggat
    12421 accatcgaat aaagtcgaaa tgttgtttta caaaatctgg gttatttaat tttttttggt
    12481 tttgacaaat aaattccaag gtttttttca ctctgtctaa agaaatctta atgacttttc
    12541 catcgtgaat cacttgggga tcataatttt tgcctttatt taagtaggct aaagtatgct
    12601 ttgctgtact gcataattca gttcccttaa tgtcatatac tccattcaag gcaggttggg
    12661 atggaaagaa ttttggcgca gcccatagtg gagaaataaa cagcactgct aaaaataatg
    12721 tcgctattcg catttactct gttgtttcct ttttctcttc tgcttttcga gacaatccta
    12781 atttttcttt taaacgggct acagcatccg gatcatattg ctttggtgct ttatgtttga
    12841 ctacgacgga tggtgacttg gcggattgca ttgccacatt ttgtgaggca taggtagagc
    12901 ttctgagagg atatattggc aaaagaggct caggatgaga gtcaaacgaa ttcattggct
    12961 taacgctgga aactgcactt ggctgactat ttacatgcga atgattagcc gcttttgcat
    13021 tcacttgttt gcgagcattt ttaacactct tttccacgcg ttttttaatt ttcatggaag
    13081 catgctcagc ttcttcctca gtaacctcgg ctactggatt tccgtgcaaa tcaatacgga
    13141 cttcacgagc tttcaggcaa gccagataat caagacgacg ggtaaaaaga acaacagctt
    13201 cccttaattt acttttagaa attccaactt gctctgcttt ttctgcatgc tgcaatatat
    13261 cagacataat accaatcttt aatggccgaa tacgcaaaga attatcgaat gcctcaggga
    13321 aattagccgc aagccataat aatgcgtcag atcgcgctcg cttggattga tttttttgtg
    13381 ctttattgat gacagcggtt cgtgggtgca gcgcctgctt tctcattctg gtacccttat
    13441 tgtgtttagt ctattggcat gaaaattatc aaaatcattt gatttgcaaa agtctgttga
    13501 ccttgaatgt atgatagtca tatcttgaag tcagatggtg taaattatgc tattagttac
    13561 tcgaggaatg agcgcacaat gtacaatgtt tattggttgt ttgcaagcct tctttattat
    13621 ttccattaag ccgaatacct tttgatttgt atccaattag caaaatacat tatgatatgt
    13681 ctataattaa cttaagtcag atagaatagc gcagaggatg cttgatagat tgctaaataa
    13741 aggaacctta tcgctcaata atgtattaat ggtgattaca aaatgaggat aatactttca
    13801 attcttgcta tatttatacc gtggttggtt ttattgatta aagacaatcc aggcggagct
    13861 gtcgttgcgt tgattatgca agcaacacta attggatggc cttttgcttc aatctgggca
    13921 tggcgggcag tcaacccact tactccgacg tctcaatcca aacaataagt atcccaatcc
    13981 tgtttttatt caacatataa ctaaatcagg acttaagaaa aatgccaagt ctaggcaaaa
    14041 aatgttttta atgagagagt ttagataggc tcaatgaccg aattaaaaat ttttttataa
    14101 agagattggg gtttttcgga aatcatgtaa ataattcgga gtgataaatg cgaacacgta
    14161 tcattgttaa tggtgccaat ggaaaaatgg ggatactagc ctgtgaaaca ttggaaaacc
    14221 atgaacagtt tgaaatagta gccaaactaa gcagacagga caatcttggg caaagtatac
    14281 tggacaccaa agcacaaata gttgttgatt tgacaagagc agactgtgtc tatgaaaact
    14341 ctttaaccat tattaatcac ggcgctcgac cggttattgg gacatcgggg ttggttgaaa
    14401 cacaaatcga tgagctcacc aaactgtgtg aaataaaaca aataggtgga attattgcac
    14461 ctaatttttc tctcggtgcc attttaatga tgattctcgc cacaaaagct tctgaatatt
    14521 tttcagaggt tgaaattatt gaaggccatc atcaacaaaa attggatgcg ccatcaggga
    14581 ctgctttaaa aactgcagaa atgatagccg cggcgcgcaa aaaacccaaa aacaaacttc
    14641 cattaaaaga attaacccct ggggcccgag gcggctctca tcatgatatt aatattcatt
    14701 cccttagact gcctgggttg cttgctcgac aagaagtcct ctttggcaac ataggcgaga
    14761 cactctccat tacacataac agtatagatc gccgttgttt tatgccggga attgtgctgg
    14821 cctgtcaaaa agtattgaat ttaaataacc tggtttatgg tctggaacat ttgctttaaa
    14881 ttgcaaaaac caggttgacc cgtattactg tcgggcatac gggtctcctt ttcacatatc
    14941 accccttgat atggaacgtt tcccaactca attttttaaa cattacacat aaaccaagcc
    15001 actgttttct cttttggaac tctcaaacaa agaagaccat tctaaaatgg cttcttctgc
    15061 caatgtttgt aatgtggttt ctgatgacag gggctgtaat aatttcctaa tacgatcata
    15121 gactgaattg gttgctgtag ttcttgatga gtcgctttct ggccgattta aaacagctgc
    15181 aaatattttt ccaatccgaa gaacattatc attgcccgag ttttcatcat gcattacttc
    15241 acgaatattg agtattccag taggtgttga gctcacatct ttatcagaga accaacgtaa
    15301 tttccaggaa tctttgcttg atttttgaaa tctgcgctcc tcattgatta aagcgcttag
    15361 tacatcataa agcattgtac acattttttc gcctagctgt ttggcaatta acaagcaatg
    15421 caattcgttt tctggtagaa aaaaggaacg caaatccttg ctaatattct tcacttcatt
    15481 atcagtggct aatcggtcat cataggctaa tgctttctca gtgcccaagc gctcgttaat
    15541 ggcctcagcg atatcgtttg gccagtacca atcaggcgtt ttgattccct ctgaacctgg
    15601 cgcattttgc cctgcaagct catgttgatg tctggagata taaagatcta caacaatatt
    15661 gatgaaattg actcgttctt gtttttcttt gggaattcca aatttaggcc aatttccata
    15721 ttttgcttta tacaaaatca tcgcatcagt atgcaataac tctactgctt ttctgtcttt
    15781 accactgaca caggagccat agctatagcc gcccatgttg agaataatca gttcttccaa
    15841 agagctaaga aataattccc tgccatcgta atcccaaaat gttgcagagc ctaaaggcga
    15901 ctctaaaacg gccttataat catcgattag cgcctgcaat ccaggcttgg aagacgcata
    15961 tttctgcgct gcttttaata aaaactcgct gtctgtatcc gttgcctgag tataataata
    16021 acgcttggca atattaaaag gatgattatg ctggaatatt tcatgcctgc gtttgctccg
    16081 ggtaaccgct tctcgggcaa ttttatacaa ctccagatca ggaggtaatt ccggtaaaaa
    16141 gtctgtaacc accgttggaa tataatctac tgcatggata gggctaatta atgtttgcaa
    16201 taacaaaggc tgctgaggtt tggcaaattc catcacctta accagattag agtcaacatg
    16261 acgctgctgg acatcctcag gaaattttaa cgaatcacgg ctggcaacat ggcttgaacg
    16321 atacctcttg tcataaaata aagtttcttt accctcatca tcgattaaat ataaactgtg
    16381 cgcaccataa ttgcctggag caggcaaagt tcggtgccgg cttgataaat aagatacgac
    16441 atcctcaatc ttttctgcat ttttgagagc atattccaaa aagtattgtt gtttctcgga
    16501 caaaacccaa taccactgtg gaagcttagg cactaggctc aaagtcgatg cgtattgatc
    16561 attcctggcc tgctcaacca taaatttttt aaactctttt aagctggatt taatctcatg
    16621 tggagtagca gacaagacgc gaatcatggc ttgctgagca gggcttaatt cattgaacca
    16681 ggaaggataa ggcggtgagt tggtatgtat ttgatttaat tccgtggtca aattcagtga
    16741 cctttttgca atatctcccc aattgccaaa aaatgtatta aagtgctgca gcgctttttt
    16801 aggatcaaca gaacttaaat tcagattaca ataataagct tgttgatagg gcggcagatt
    16861 tttaaaccag gcaggtgttt taggaaaatt atattctttt aattgtttta attcacttag
    16921 ccattgttca taaacgggag aaagggattc atcatattgc aaaacatact ttgtctcact
    16981 gcccatttca ataggcatta tggtagcgat atcgctacga ccccgactca ttaagacata
    17041 ctgctctgct tcattaaggc tggctatcgc ctctttgact gccttacctt taggccattt
    17101 ccacgcgatt gcaatagctt caaccaattt ggccgtaaat cgttcgagct caagatgcaa
    17161 ctcttctaat gtcagttttt ccaggtcaca tgaagccttg cattctgcgg cataagtact
    17221 taaggttgca accgtcagag aagtatgttt tggatcgagg gggtaagctt ttaagataaa
    17281 tgaaaaggca tcatgaaggc tgtctatccg gcgagcgatg cttttacgat aatctggctg
    17341 ctctttgaaa tactctactt tgtaaccagg tagaagtcgc tctagtgtat caaatacctg
    17401 aggagataat attgtatttt ccaaaacacg taaaattata ccttttccca tagcttaatt
    17461 cccttactgc cttaatttcc tcattattgt ataaataatt caaagtgaca atgttttgaa
    17521 ttaacaattg aattatatat ttaccctaag atatcaggaa gttaattgcc ttaatagcat
    17581 tacttcatgc taaactagtt tttttggagg aatagtaaaa ggatagctat gaaaagtata
    17641 gaggatttta caggtttttc agaatccggg attgatgatg ccattcaaaa tgccttaaaa
    17701 aaagcaggta atccaactca ttttgaagtc gttgaaacac taggttcaca agatagccaa
    17761 tcagatcgac gatatcaggt aacgcttaaa acactgagtg attaatcatt aagtaacggc
    17821 aatcattggc tagaaataaa tgacaaaaat ttaatgtcaa ttctgtttct ataactatta
    17881 tctagctact gcctttcaat acaaggagtt taaaaatggg atacaatgtg cctcactata
    17941 ttggtggaca acttattaat gaaacggata ctgaagctca agccatttat aatcctgctc
    18001 tgggtgaaat tattggccaa gtccattttg cgtcaaaggc aacctgtgat agagctgtgg
    18061 ctattgccaa agcggcagga aatgaatggt cgcaaacccc tgctataaaa agagcgcgag
    18121 ttttattccg atttagggag ttgctggaaa aataccaaat ggatctggca cgaatagtca
    18181 ctcgtgagca cggcaaaacg ctggatgatg cgaaaggatc ggtggccaga ggcattgagg
    18241 tagtggagct gcactgcggg ctcgtcaacg agctaaaggg agatttttct gctgaagtgg
    18301 ccagccatat tgattgccat acttttcgac aacccttggg agtatgtgcc ggagtatctc
    18361 catttaactt tcctgtcatg gtacccattt ggatgatgat tcctgccatt gcttgtggaa
    18421 atacctttat tcttaaacct tccgagcaag atccctctgc gcctgtccgt ttattggaac
    18481 tgttaagtga tgccggtttg cctccgggcg ttgccaattg tatccagggt aataaaacga
    18541 ctgtcgagca tttactggct catccggata tagctgcctt tactgcggtc gcctccactc
    18601 ctgttgccga atacatctat accactgcag cagcctatgg aaagcgggca catacttttg
    18661 gcggagctaa aaatcactgt gtggttatgc ctgatgccga tttagaccag gcagctaacg
    18721 ctatcgtcgg agcagcctat ggttcggcag gagaacgttg tatggcttta tctgtcgttg
    18781 taacggttgg tcaacatacc gctgatgcct tgatcaataa aatttccccc ctcattcgag
    18841 ccattcgcgt agacgccgga gatgttgagg gcactgatat gggtcctctt gtcagccagg
    18901 ctcatcgaca aagagttttg gccgctattg aaaaaggggt taatgaaggt gcccgcttga
    18961 ttatcgatgg cagaaccttt caacatcctc aatacccaca gggtttttac ctgggaccct
    19021 gcttgtttga tgaagtaact gaagaaatgt ctgtctatca aaatgaaatt tttggacctg
    19081 tactggttat cgtgcgagtc aaccattttg aagaggctct tgctttagtc aataaacatc
    19141 aatacggaaa tggcactgct attttcactc gggatggctt tacagcacgt gagtatagcc
    19201 agcgagttca agcaggcctg gtcgggatta atattccaat acccgttccc attgccaacc
    19261 atcctttcgg aggctggaag cgctctgtgt ttggagatac caacatgcac ggagaagaaa
    19321 gtattcattt ctatactcga cgcaaaacgg ttaccagcaa atggccaata accgaattaa
    19381 aggatagcag ctttgccatg cccactcatg attaatcatg cttattacgg agaaatatat
    19441 ggctaatatt ggatttgtgg gattgggtca tatgggtttt ccaatggcca ttaatctttt
    19501 gaaagccggt catagtgtca tgggctatga tttgcaacaa tcagcacttg agcattttgc
    19561 tgaacaagga ggaactattg ctcataactt gcaggaattg gcaaaaggca aagatgtcat
    19621 tgttactatg ttacaaaccg gacagcaagt tttacatgta tgccttggtg tagatggtat
    19681 attcagtcag ttaaatgctg gctcattgta catcgattgc tctacaattg atgtaaaaag
    19741 ctctcgagaa actcatcaaa tagcgaaaga aaatcatctt ctggtcgtcg atgctcccgt
    19801 ctctggcggc gtagcggggg ccactgcagc cacgttaaca ttcatggttg gtggtgaagc
    19861 cgaggcttat aatgcggcgc atcccattct ctctgccatg ggaaaaaaaa tcatccatac
    19921 aggcggtgca ggcagtgggc aagcagccaa aatttgcaat aacatgatat tgggtaccac
    19981 gatgatcgcc atatcagaag cgttcattct ggcgaaagaa ctagggctgt ctgatggaaa
    20041 attatttgag gtggtaaaca attcatcggg tcaatgttgg gctatgagca aatacgtccc
    20101 tgtaccagga atattggaaa atgttcctgc aaacaatgat tataagccag ggtttgcagc
    20161 aaacatgatg ttgaaggatt tgttattgag tcaaaatact gcgaaatcag tacacattga
    20221 aacacccctt ggcgccaaaa caactgaaat ttatcaacaa tttgtcgatc aaggccttgg
    20281 tgatatcgat ttttcagcca ttattaaaca cattggcaaa atacaagagg tgtagcatgc
    20341 ttcccgttca tctaaagcac cctcatttaa cagctacaga agaatatcta tctgaatttt
    20401 ggtctgaaat tgccgcacaa accattgact ggatacaacc ttggagtatc accctgcagg
    20461 gaggattaca caaaggcgat gtgaagtggt ttcaaggcgg cttactcaat gttagcgcta
    20521 attgtctgga taggcattta ccccataaag caaatcaaac agccatcatt tgggaaggtg
    20581 atgatgaaaa ccaaaataaa accctgactt ttgcgcagct ttattctgaa gtatgcaaaa
    20641 tgagcaatgt gttaaaaagc ctaaatgttc gcagagggga tacggtaggc atttacctac
    20701 ccatgattcc tgaagcggcc attgccatgc tggcttgtgc tcgtatagga gctattcata
    20761 ccgttgtgtt tgccgggttt tcggcccatg cattgcagca gcgattaata gcctcttcct
    20821 gtaaatgttt aatcactgcg gatgcgtttc aacgcggtgg aaaaactatc cctttaaaga
    20881 aacaggctga cgaagccagc gtggacttaa atatcaccaa gctggttgtt aaaaattcaa
    20941 atgccccaac ggcactaaat aagaacaaag aacattggtg gcatgagcta aaacaaaccg
    21001 tatcggatca atgcacgccg gaaccaatga acaccgaaga tcccctgttt attctctata
    21061 cttccggaag cacgggacag ccgaaaggag tggtacatac cacaggcggc tatttagtgc
    21121 aggccgctta tacgcatcaa ctgatatttg cttgtcagga taatgaagta ttttggtgta
    21181 ctgctgatgt cggttggatt acagggcata gttatgtcgt ctatggccct ctctgcaatg
    21241 gcattaccac attgatgttt gaaggaatac ccacgtggcc tgacgcagca agaaactggc
    21301 gcatcattga taagcaccaa gtgaatgtgt tctacacagc tcccacagcc atccgctccc
    21361 tgatgagagc tggcgatcaa tggctaaact caagctcaag aagttctctt cgtttactgg
    21421 gttcagtggg tgagccaatt aacccggaag catggaattg gtatcaccaa aaagttggac
    21481 aagggaaatg cccgatagtg gatacatggt ggcaaactga aaccggagct attatgatta
    21541 gtcccagagc cagtgatgaa gtgatcaaac caggctctgc tcgtaaacct attccgggga
    21601 tcgtaccact cctgttaaat gagcaaggac atgaaattaa tggcgctgga gaaggccttt
    21661 tggctatcaa atacccatgg ccttctatgg caagaaccat tgcaggagat caccaaagat
    21721 actgtaacac ttatttatcc aacggctatt acatcactgg tgatggagcc aaacgagatg
    21781 aagatggaga ttattggatt acaggccgaa ttgatgacgt acttaacgtt tccggccatc
    21841 gattgggtac tgctgaaatt gaatcagcat tggtcagcca tcctaaggtt gctgaagctg
    21901 gcgttgtagg tattcctcat gatttaaaag gtcaagcaat atttgcctat gtcattctga
    21961 agcagggaaa taagcctgat gccgagctcc aaactgaatt gatggagcga gtcaaagatc
    22021 aaatcagtgc tatcgccaaa ccagatgtca ttcagtttgc aaatgatttg ccgaaaaccc
    22081 gttcaggtaa aattatgcgc cgaatactaa gaaaaattgc ttgtaaagaa gtgtcgcaca
    22141 ttgatgagct gggtgattta acaactcttg ctaaccctca gatcgttgag gaactcatga
    22201 aaaatatttt gaagcggtaa aaagaaccgt cgagcttcac aaagtttatt ctgatagcaa
    22261 acaaataaac tatgtgaagc ctgacaacca gttcaaataa tatcaaatca atttcatcag
    22321 gcactgacag agagcggttg cattgacaat gaaaaatctt tgacaatttt ttcagactcg
    22381 gtgggtacag gaaataatcg ccatctcccc gcgtataaat tggctccgcc caaagtacat
    22441 aaagtgacta aagcactcgc taaatcagca aacacttgtt tccagccacg atgtacttct
    22501 aaaacaggca tgactgcatt aatggcacgg acgcaagctt gatgaaaatc attgcatttc
    22561 acattcttag gcagttgaga gtttttgaag tgctcttcgg cccttaataa attattgtaa
    22621 aatgttctcg ctatgggtgc cgcgcgttta taattttcat cgccaaccgc tttggtttcg
    22681 agcgttttaa ccatactttc aatagttttc aagtggtcgg aaaatttaat ttcggcaaga
    22741 atattcaaag tttcttttat tgcagcgatt tggtcagtaa tacgcttgct cattacatca
    22801 gcttgtctgt taatttcaca aattttatct gtcagagcta ttttgatatc actatgatac
    22861 tcatcagata gttgaagctg ttcccgcgcc ttaagtaaat ctggtttgtt atccaccaaa
    22921 tatcgaaccg attgaattaa atcctgtttt ctggcaatca cttcttcaac tgtttggcac
    22981 ctgctaaaac ttatcggaaa ttccctgatt tgtttttcat agctttttac tacttcttca
    23041 gcatttattt taatgatcaa ttgatcagct gtttcattta tttcttgttt tttcgcttgc
    23101 agtgctctta ctatggatgg ttgcaagtta ttacaatcca gaaccttttc agaacttaac
    23161 aaagcttgat taggtttaac cagattatca agttgttgca ataaattatt acgctgccat
    23221 tctactgcag aagtggagct tatatcatta aagcgaatta caaaattatc aattcttttt
    23281 tcaacagaat cgataacctt ttgcgcctca tcaagaagcc gtttttgcaa taattcagcc
    23341 cgcaaattaa tcttttcaac ctgggcataa tagaccattt ttatttttgg attggcgatg
    23401 aattgttcgc ccaaaagatc ttccgctttg accagtgtgt gaagcttttt gttcaacaga
    23461 atacgttggg attcaatagc tactatggta tccgcatcat taaagtcgat tgggaaattt
    23521 tttatttgcc tgacatgctc atctatatcc gtttcatact tcctgagaaa atctgaacgt
    23581 cgattagctg cggcgtctat cattcggagc ttacgttcta aagccctctg gacttccgga
    23641 tgtccatcag gtaaatccag tatttgcaac gcattttgca attcgccaga atgaactatt
    23701 ttcttaagtt ggcctctgag ctcttctgca ttaagattaa cgtgttctac ggttttaaat
    23761 ccttcaaaac taaatggaaa agaattgatt cgctgaacaa tatctgaaat cactctttta
    23821 gcatcgtcga ctcgttgttg ccttaataca ttatcaattt ctggaatttt tatcacaggt
    23881 tgtggcttgg ataaggactc aaaccatgat ttgaattcgc ctgaatcttt taaagcgatt
    23941 ttttttgcgt attcttctaa agatatgcca aatagaacat taattttttg agatgcgaag
    24001 acaaataacg cttttgccac ttctggatta attcggctta atctgaacaa attttcaaag
    24061 aattcatccg gatctttaaa cgttgcgctg gcaagaacga catcaaaaaa ctcctcatca
    24121 attggcgatg tgtctttgct ttttaagtac tcaatcaata aatctggtaa agtctttagt
    24181 gtgagatagt tagcaatcgc ttttgcaaaa agagcttttg gcggaaataa tgcctctatt
    24241 gattttttgg agaaaatatt ggctataaac aatgctcgta accctgatgc tcttgttaaa
    24301 ctagaggatg ctccaccaaa gtaaaagtta attaacccgg attcaatttc ttgtttcgct
    24361 ttttcaggca ggtaagctaa ctgattgatt ttttgctgta ttgcagactt cactattaaa
    24421 tggagaaaag cctcgccatt aaaatttatg cctgctttgg gacttaattt gttttcttcg
    24481 actgggtttg ataaggctaa ctctgtcaaa acctcgccta ataattcttg agatgtttc
//
LOCUS       DACTJE010000031        24128 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.031, whole genome shotgun sequence.
ACCESSION   DACTJE010000031 DACTJE010000000
VERSION     DACTJE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24128
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.031"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..89
                     /locus_tag="I9760_000968"
     CDS             <1..89
                     /locus_tag="I9760_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT5042746.1"
                     /translation="RKMKLIQRRAYGFRNFENYRLRVKVLCS"
     gene            complement(199..275)
                     /locus_tag="I9760_000969"
     tRNA            complement(199..275)
                     /locus_tag="I9760_000969"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(239..241),aa:Ile,seq:cat)
     gene            complement(365..2230)
                     /gene="rpoD"
                     /locus_tag="I9760_000970"
     CDS             complement(365..2230)
                     /gene="rpoD"
                     /locus_tag="I9760_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAT5042747.1"
                     /translation="MTMNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHF
                     DVIISMLEGMNIKVFELPPSEDELALLLNTEEVPDIEEAAMVLASVDKETGRTTDPVR
                     MYMREMGTVELLTREGEIRIAKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIR
                     LNEIISGFADVDEEIAPATNIGSMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPE
                     QAKIYFDELREAFEKAMKALKEHGRTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHF
                     RQLRNHIREFERSIMRLCIEKARIPRKLFIDTFPGQETDLGWLETIISKHAKKLDLQR
                     IEENREEILRLQSKLASFEIEYGLTISEIKDINRKMSIGEAKARRAKKEMVEANLRLV
                     ISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIAD
                     QARTIRIPVHMIETINKLNRISRQILQETGREATPEELAEKMELSEDKIRKVLKIAKE
                     PISMETPVGDDDDSHLGDFIEDNNIESPIDMATAEGLREATLEILETLTPREAKVLRM
                     RFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEKLRSFLEGDEG"
     gene            complement(2317..4050)
                     /locus_tag="I9760_000971"
     CDS             complement(2317..4050)
                     /locus_tag="I9760_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAT5042748.1"
                     /translation="MSGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHN
                     EKTPSFNVVAKKQFYHCFGCGASGNAISFVMNYLNQGFTDAVETLATRLGLSVPRDGT
                     GEKHKSSLNLYNLMSEVSQYYQKKLKYNGQVAIDYLRNRGLSGEIAKRYHLGYAPEGW
                     HNLEKAFPNNQRELLSTGMLIKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRVLDKD
                     QKPKYLNSPETVLFQKSRELYGLHQVLSQQKNPDSIIVVEGYMDVIALAQHGIFNVVA
                     TLGTATSTFHIQLLAKHTKHLIFCFDGDAAGKQAAWRALESSLPHLNAGLDAGFIFLP
                     NEHDPDSLVRNEGKNGFLELLQQATPLHRFFFDTLSKDINLSNPAGKTQLINAVKPYL
                     QKMVEGSYKQLLIDDLSRLTHIESHRLTNLITDKSESKPEVQAAISRSPLRIAIALLL
                     QNPEIYSIAIQQINPALLHEQEHHILLKLLQQLKDKPNANTATLIEFWRNSSYFELIV
                     KLAAWDHQVPEQELTKEFIDVLLFLQKQNREILIRQYIEKSRKTGLTEAERLSLQNLL
                     KERHGQAEIEK"
     gene            complement(4059..4502)
                     /locus_tag="I9760_000972"
     CDS             complement(4059..4502)
                     /locus_tag="I9760_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2359 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042749.1"
                     /translation="MTIKEQINNDIKDAMRAKDKNLLNALRLISAAVKQIEVDERIEV
                     DDERMLVILDKMSKQRKESIAQFEKASRDDLVAQEQFELNIISKYLPEPLTDNEIEEL
                     ICDAIKSTGAEKMSDMGKVMAILKPKLQGRADMAQVSARIKAKLS"
     gene            complement(4705..4944)
                     /gene="rpsU"
                     /locus_tag="I9760_000973"
     CDS             complement(4705..4944)
                     /gene="rpsU"
                     /locus_tag="I9760_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAT5042750.1"
                     /translation="MPTVRVKEGENPEYALRRFKRSCEKAGILTELRRREFYEKPTAE
                     RKRKQAAAVKRHLKKISRDVSSRRGVGHRRKKSTT"
     gene            5149..6150
                     /gene="tsaD"
                     /locus_tag="I9760_000974"
     CDS             5149..6150
                     /gene="tsaD"
                     /locus_tag="I9760_000974"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAT5042751.1"
                     /translation="MLVLGIESSCDETGVAVYDSESGLLSHALHSQIATHRVHGGVVP
                     ELASRDHVNYLVPLVDEVLTKAQIRKNQLDGIAYTAGPGLIGALLVGSCFAKSLAYAL
                     NIPALAIHHLEAHLLAAKMETPSLDFPFIALLVSGGHCQLIEVNNIGEYRLLGDTLDD
                     AVGEAFDKTAKLMGIPYPGGAVLANLADQCLSTPYQFPRPMTDRPGLDFSFSGLKTHA
                     LNTWNQSEKKESDRSEIAKAFQQAVVETLIIKCKRAIKESQSKRLVVAGGVGANKALR
                     SALQKWIKDIQGEVYFPALEYCTDNGAMVAYAGCLRMMRGEIDGGLGVIVKPRWPLA"
     gene            complement(6324..7157)
                     /gene="plsY"
                     /locus_tag="I9760_000975"
     CDS             complement(6324..7157)
                     /gene="plsY"
                     /locus_tag="I9760_000975"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAT5042752.1"
                     /translation="MALFIFLILVGYLMGSINSAIIVCRTFGLPDPREEGSKNPGATN
                     VLRLGGKQYGIMVMVFDALKGILPVILAKFLSAEPVTVAFTALAAVVGHMYPVFFHFR
                     GGKGVATTIGALLAFHFIIGVMVAATWLLVANFWRYSSLASIASISLAPFYSLILVGN
                     LNIFPPLFMITILVLYKHRDNFNRLIDGKEPKIKFKHSVIEEIMEASPATSAEQEFPG
                     KEVIDTNIDETEKTEQAEAVKKPKVKKATTKAKKTTSKEETTKKPKSTKPKTKTVKEK
                     E"
     gene            7511..8920
                     /locus_tag="I9760_000976"
     CDS             7511..8920
                     /locus_tag="I9760_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAT5042753.1"
                     /translation="MDAIHLLSATEIIRRIKMKELSAAEVMVAHLNHIDKINPVINAL
                     TERIPPEECLKQAKEIDKSISSKKNLNKLMGLPVAIKDALYVKGLICSSACSGFYKGE
                     KAVRDATLVSRLKKEGAIILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGG
                     SAALIAAGGVPFALGSDGGGSLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNL
                     ISFGPMARSVSDLRLGLSVLAGSDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVD
                     AEIQNVVKSAALALQDDVAIVREVRPDCVSKAFDLHWELFLGGDRGAGFKTMLSELGV
                     NNLSWELQEFLRQAEQTQFSVTQLHQRMREIDLFRLELALFMQDYDVLISPVFPTVAK
                     PHGIGIKEISDFSYAMTHNLSGFPTISLRCGTSAEGLPINVLIAANRWKDTTSLAVAE
                     RVEQLMGGYKPPSLIHSLQ"
     gene            complement(8952..10079)
                     /gene="hemW"
                     /locus_tag="I9760_000977"
     CDS             complement(8952..10079)
                     /gene="hemW"
                     /locus_tag="I9760_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAT5042754.1"
                     /translation="MLPLSLYIHIPWCIRKCPYCDFNSHKSPDVLPEHQYIQALIEDL
                     KTDISSYNTREINSIFIGGGTPSLFSAEAYNNLFNELKRILPFAKNIEITMEANPGTV
                     EQHRFTDYRQSGINRLSLGIQSFNPNHLKILGRIHDEKQAHSAIDTARKAGFDNLNLD
                     IMHSLPNQSVTQGLQDLKTALSYQPEHLSWYQLTIEPNTVFYKHTPPLPSEEEDYLLE
                     EQGFALLHNSGYHRYEISAFSKPEKQARHNINYWLFGDYLGIGAGAHGKMTTPNAIIR
                     TRKHRQPKDYLDNEKPYLAACETITGKELLFEFMLNTTRLEQSIPLDLFTTTTGLELN
                     QLLPLLKLANKKQLINLTDTHWQVTPLGRQYTNDLQALFLP"
     gene            10161..10721
                     /gene="nudE"
                     /locus_tag="I9760_000978"
     CDS             10161..10721
                     /gene="nudE"
                     /locus_tag="I9760_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAT5042755.1"
                     /translation="MRNKPKCKQRTVIAKSRLFTVEEMYLEFSNGAQRVYERIKSHGH
                     GAVLIVALTPGESLLLVREYAAGTDSYELSFPKGVIDRNETPIEAANRELREETGFAA
                     RELHWIRSMSLAPGYFGARIELIVAEGLYHSPLPGDEPEPPETVEWPLHAAYELLEKP
                     DFTEARSIAALFLVREWLNKRKSYDK"
     gene            10711..11703
                     /locus_tag="I9760_000979"
     CDS             10711..11703
                     /locus_tag="I9760_000979"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAT5042756.1"
                     /translation="MTNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLEL
                     EPALPSLEGVAMELDDCAFPLLKRIVCTADLNKAMDGVNWALLVGSVPRKQGMERSDL
                     LQINGGIFTKQGQAINDYASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLD
                     ELRARTQLAKKAGVDITAVTQMTIWGNHSATQYPDFYNAKINGTSAAQVINDETWLKE
                     TFVSTVQQRGAAVIKARGSSSAASAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGV
                     DEGLIFSFPCRREHGELKVVENLEFNDFGRERFNTTLNELRSERDTVKSLGLLD"
     gene            complement(11802..12722)
                     /gene="mavF"
                     /locus_tag="I9760_000980"
     CDS             complement(11802..12722)
                     /gene="mavF"
                     /locus_tag="I9760_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavF"
                     /protein_id="HAT5042757.1"
                     /translation="MQNIWLLCFYKLLLKKGLWRNIGMEDPFSVLLKSLQSVFHLEAM
                     RNGKYTFPAVQHLKEATENYNPKARNTFIELLRAIREALPYIEKWRINFNVIRKSMDA
                     LAKLHHMPTIDWNQVLSHPKVSPRFQFSALNHSHHDYDLTTWLEKKVGKPFAQLDHTE
                     LTQTLVDNRDRFGFSQKLSNHLKKDPDFLYTIILRSERNFIKISQTRLILYLTDEQLA
                     RAIIKHIPVLMHKRKEPFEQIEQLIHTLNEILSNGRSVSTLLRNTDAKTILENSPLFQ
                     MYLSEEYKNRHQHPNKDPDLKTNESLKPGL"
     gene            12853..13392
                     /locus_tag="I9760_000981"
     CDS             12853..13392
                     /locus_tag="I9760_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAT5042758.1"
                     /translation="MITVGNKFPEFQLKATVANDVSNAFQMISNETYRGKWLVVFFWP
                     KDFTFVCPTEIAEFGRLNSEFADRDAQILGGSTDSEFVHLAWRNQHPDLNNLPFPMLA
                     DVKRELTANLGILDDEEGVAQRATFIVDPQGITRFVMVTDLNVGRNPAEVLRVLDALQ
                     TDELCPCNWKKGEETIHIK"
     gene            13415..13930
                     /locus_tag="I9760_000982"
     CDS             13415..13930
                     /locus_tag="I9760_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase"
                     /protein_id="HAT5042759.1"
                     /translation="MLENVKESIPEVAKDIRLNLTKVLDLEQSDGLSEHQILGSALAV
                     SYSLGNEVLIKGLKELVHNEQIVRAAQLAASLMAMTNIYYRFVHLTEIKEIEHIPAGL
                     RMQGMMTPGVDRVTFEILSLAVSALNGCGACVSSHARQLKEHGLSDTSLARVGRITAV
                     IHAVHVSGRIK"
     gene            complement(13974..14462)
                     /locus_tag="I9760_000983"
     CDS             complement(13974..14462)
                     /locus_tag="I9760_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn]"
                     /protein_id="HAT5042760.1"
                     /translation="MNKSGIILIGTILFSSMAIADDLTAPIYTTGPKPVAIGKVTFTQ
                     TPYGVLITPDLTNLPEGPHGFHLHKTADCGNHGMHAEGHYDPQNTNSHQGPYGNGHLG
                     DLPVLYVTSNGKAMIPTLAPRLKLSDMHNLAVMIHANGDTYSDNPPQGGGGDRIACGV
                     IK"
     gene            14767..16791
                     /locus_tag="I9760_000984"
     CDS             14767..16791
                     /locus_tag="I9760_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAT5042761.1"
                     /translation="MELRIDNTPFKAIFQPLDLGFTQIKNRLLMGSMHTGLEEDKENL
                     NRLASFYRERAQGGVGLIVTGGFAPNRAGRLAPFAAKLTNSKEQHRHEVITQAVHEAG
                     GKIALQILHAGRYGYHPFIVAPSGIKSPISPFKPWEMSKRRIIKTIKHFARCARLAQL
                     AGYDGVEIMGSEGYLINQFIVTHTNHRRDEWGGSFSNRIRFPIEIVRSVREAVGEHFI
                     IIYRLSMLDLVAEGSNWQEVVTLAKAIEQAGASLINTGIGWHEARIPTIGTMVPSAAF
                     TPVTQHLKPEIKIPVITSNRINTPELANQLLEDGVADMISMARPFLADPCFAEKAKLG
                     ESKAINVCIACNQACLDQVFVNKTASCLVNPRACNETTLVYETTNQPKAIAVVGSGPA
                     GLAFAVTAAERGHKITLFEKANQIGGQFNLAKKIPGKEEFQHTLNYFAYQLQKYKVDV
                     RLNTEATVELLKDYDEVVLASGIIPRVPEIKGIDHKKVVSYIDVITQNKPVGSRVAVI
                     GAGGIGFDVAEFLTHDHQLTSEQFYNEWGIDISGQHRGGLKPPEIAPSPREVYLLQRK
                     KEKMGKRLGKTTGWIHRSSLKHKQVKMLSGVNYERIDDQGLHLTIDDKPQVLEVDSIV
                     ICAGQIELKNLYEPLKMAGKLVHLIGGAYKALELDARHAIDQACRLAALV"
     gene            16788..17705
                     /locus_tag="I9760_000985"
     CDS             16788..17705
                     /locus_tag="I9760_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAT5042762.1"
                     /translation="MSKIAVVINPIAGGGRGKKVWKVLKPGLHALFEQTVYRISNRVN
                     DLEAITDGLLAENPDSLLIIGGDGTLNHVLNGLIEKDKLKNPQTKIAFFNAGCGGDFT
                     RQFPQQKMTEFLDRLSHNQFIKCDIGKITLANQLSRYFINIASCGLSGHVVLRVSKSK
                     WLKKLGGTLNYLLHALTGLMTYRKTKVRVQIDDALPFDSSILLMAVCSGQYFGGGMHV
                     APMAKVNDGLFDVVSFNEFSKLNALLKLYKIYSGGHLLDNNVHYVQTKKIKIEPLEES
                     KIEIEADGEIIGCLPAQFELIKETLPLII"
     gene            18073..19842
                     /locus_tag="I9760_000986"
     CDS             18073..19842
                     /locus_tag="I9760_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAT5042763.1"
                     /translation="MNQEISNFSTFNFSNALNKALEDMKFITPSPIQAQTIPLILQGR
                     DAIALAQTGTGKTAAFALPILQNLSPEISTTQALILAPTRELAIQVAEQFELLSKYQR
                     NVTIAVLCGGQEYGRQLKQLRSGAQVVVGTPGRILDHIDKGTLLLNNLKTFILDEADE
                     MLRMGFIEDVETILEKLPEKKQMALFSATMPYRIRQIANTYLNDPASIEIRMETATVK
                     SIEQRFLFASVHQKPDALIRVLEVEDYQGVIVFVRTKSSTEEVAELLQQHGLRAMAIH
                     GDITQSLRERIIAQFKQGAIDILVATDVAARGLDVERVTHVINYDMPHDNETYVHRIG
                     RTGRAGRSGVTILFVTPKESRLISSIERHTRQRIEKVQVPNDHMIQMARQQRFMSNIT
                     TRLNHEHVYSYKRIVEEYIKEHDVSAVDVAAALALLLHKDMPWRKELNMPKAASAFKE
                     ERARAGEKGLDLRGRFKEDRKSSFADRKGARGSKKDFAKDFSNERKKFNDGRKAVNQE
                     SVAQDLFRIDVGKIHGVKPGNIVGAIANEAGLQSRYITGLKIHEDHSTVRLPKGMPKE
                     VMKDLTKAWVCGRQLNLTLIGSA"
     gene            20192..20410
                     /locus_tag="I9760_000987"
     CDS             20192..20410
                     /locus_tag="I9760_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042764.1"
                     /translation="MKKKHEPGFFGRETKNIKDIESNRYDPDCPIRRADKSRGFSQKA
                     SSQFQLRIKEEVKEALDDIRNESAYKFN"
     gene            complement(20570..21196)
                     /gene="mavE"
                     /locus_tag="I9760_000988"
     CDS             complement(20570..21196)
                     /gene="mavE"
                     /locus_tag="I9760_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavE"
                     /protein_id="HAT5042765.1"
                     /translation="MTRFIMLSFVTGYRKSISCNRFSRNVVSPYFFMRTIIMTRFERN
                     FLINSLMFLETILSVDKKLDDAIHHFTQGQYENPRYQINSRITNADDWSKEDKLKFTS
                     AIAEAIALVSEKYENPTSETTEQIQSARNILLDNYVPLLTANTDPENRLKSVRENSSQ
                     IRKELIAKLKDEVPYKSQFENPYVLFPFVAATVAVAATAASVLFGNKP"
     gene            21355..22284
                     /locus_tag="I9760_000989"
     CDS             21355..22284
                     /locus_tag="I9760_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAT5042766.1"
                     /translation="MKLLASLCAFLLSGAVSATPLNNIVVFGDSLSDNGNLYEYMKHQ
                     LPQSPPYFEGRFSNGPVWIERLAASYFPNDPNSHLLDYAFGGAGVSVDEEDDEVFFTL
                     RREVNSYLLAHQDKASPDSLFVIWIGANNYLGMPVEVDETLKNVNRGITDSIQRLVDK
                     GAKHILVLNLPDLGRTPAALEFGSVEEMTYFSTQHNNALSNTVDYFKKTYPEVEWLFF
                     DTGSHFDHVIEHAAEYGFTNITGTCSFSIVDEITKNSVLKMVASVKPELTESACDGYL
                     FFDLVHPTALAHKIMAEKARLMLDEAGVEFAEN"
     gene            22476..23249
                     /locus_tag="I9760_000990"
     CDS             22476..23249
                     /locus_tag="I9760_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3530 family protein"
                     /protein_id="HAT5042767.1"
                     /translation="MNQIKWIVLLFCFVVSNVFADKLDITVDQKKIILPYWLSRPVSY
                     GAVIIVSGGETAQWSLLLEQFAKGLARNGWSVVLLNCTKNNSIPWINQLPEVISTLRQ
                     NNNKRIVLVHYGDQLNLALEYFSKPQSKMINGLVMLSAYDLNRNLQKVPRLRFPLFDI
                     VGQFDYDMVRQQRKSREEKFKENSYLAIDIPGATHDYQYSQQLLLAFVHGWMAKLPEF
                     EPQPPPILVSYLEPVYSTASSIVSTNKLDYQPLVSQINS"
     gene            complement(23216..24106)
                     /gene="djlA"
                     /locus_tag="I9760_000991"
     CDS             complement(23216..24106)
                     /gene="djlA"
                     /locus_tag="I9760_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAT5042768.1"
                     /translation="MNLRDFFVITTWWGKILGAFFGYLTAGPVGALFGILVGNFFDRG
                     LVSYYSNPHWLYHAEKQRIVQKAFFEATFSIMGHVAKSDGRVSEQEISMAKSIMNEMK
                     LSKGQKDLAKRLFNEGKQADFNVSLALIQLQRICKDNRDLLKLFVDIQYRAAQVDGLS
                     SQKIHALDNIFTHLGFAPLHKQYRFYEDFGSYFQQEQSKQHYHNQQEYKHTSSSQGQQ
                     GYKPQSPPNTLAHAFALLEVSPNANKQEVRRAYRRLLSRNHPDKLIAQGLPEEMIKLA
                     NDKTHQIMKAYELICETKGW"
BASE COUNT         6985 a         4412 c         5182 g         7549 t
ORIGIN      
        1 atcgtaagat gaaactgatt caacggcgtg cttatgggtt tagaaatttt gaaaattaca
       61 ggttaagggt taaagttttg tgttcttgat tagcgccccc ggaaatggga agagccaaga
      121 cccctttatt ttctgtctac attgcgtcta catgactttt tcgaattttc taaaactacc
      181 gtaactactt gattttattg gtgggcccac taggacttga acctaggacc aacggattat
      241 gagtccgctg ctctgaccaa ctgagctatg ggcccggaga gggggtcatt ttaaacttaa
      301 tttctttaat gttctactat tttttatatt tttttaatga ctgtttgtaa aaacagtcat
      361 taaattaacc ttcgtctcct tcaaggaagc ttcttagctt ttctgaacgt gaagggtggc
      421 gcaatttgcg tagtgctttt gcttcgattt ggcgaatacg ttcgcgggtt acgtcaaatt
      481 gtttaccaac ttcttccaga gtatggtctg tattcatttc gataccaaaa cgcatacgca
      541 gaaccttggc ttctcgtggg gtcaaggttt ccagtatttc taaagtggct tctcttaaac
      601 cttccgctgt agccatatcg attggcgatt caatgttgtt gtcttcaata aaatcaccca
      661 gatgagagtc gtcatcatca ccgacagggg tttccatgga aataggttct ttggctatct
      721 ttagaacttt gcgaatttta tcttcactta gctccatttt ttccgccaat tcttccggtg
      781 tcgcttcccg gccagtttct tgcaaaattt gccgagaaat acgattgagc ttattgatgg
      841 tttcaatcat atgaaccgga atacgaatag tccgcgcctg atcggctatg gatcgtgtaa
      901 tcgcttgtct aatccaccag gtcgcatagg tagaaaactt atagccacgg cggtattcga
      961 atttatcgac tgctttcatc aggccgatgt tgccttcctg gatcaaatca agaaactgca
     1021 agccgcggtt ggtgtatttt ttggcgatag aaatcacgag acgcaagtta gcctcgacca
     1081 tttctttttt agcacgtctt gctttggctt cgccaataga catcttgcgg ttaatgtctt
     1141 tgatttcact aatggttaaa ccatattcga tttcaaaaga ggccaacttg gattgtaagc
     1201 gcagtatttc ttcccgattt tcttctatac gctgaagatc aagttttttg gcatgtttgc
     1261 tgataatggt ttctaaccag cctagatctg tttcctggcc tgggaaggta tctataaaca
     1321 attttctggg gatgcgtgct ttttcgatgc aaagacgcat aatgcttctc tcaaattcac
     1381 ggatatgatt tcgcaattgg cgaaaatgac gagttaattt atcaacttgt ctggaagtca
     1441 atttgagttt cagaaaagat tccgacatgg aatcgagcaa ctcgaccgtt tttttattcg
     1501 tacggccatg ttctttgagg gctttcattg ccttttcaaa ggcttcacgc agttcatcaa
     1561 agtaaatctt cgcttgctca ggattgggtc catcatccac atcagatata ccaccttcgc
     1621 cgtcttcatc ttcctcatcc acatcaagaa gtatttcttc ctgttgctct tcatccagca
     1681 tggagccaat gttagtggca ggtgctattt cttcatcaac atccgcaaaa ccactaatga
     1741 tttcattaag gcgaatttct tcggcaagga aacgatcata gtcttcaaga accagttgca
     1801 ctgtttccgg ataatgcgcc agggatttta ggacctggta aattccttct tcaatgcgtt
     1861 tggcaatgcg gatttcacct tcacgagtca gcagttctac tgtacccatt tcgcgcatat
     1921 acatacgaac cgggtcagtt gttctacctg tttctttatc cacagaggcg agtaccatgg
     1981 ctgcttcttc aatatctggg acttcttcag tatttaataa gagcgcaagt tcatcttcac
     2041 tgggtggaag ctcaaaaact ttgatgttca tgccttccag catgctaatg attacatcaa
     2101 aatgctccgt atccacaata ttaggtaata agtcattaat ttgactgtaa gtcaaataac
     2161 cttgctcttt acccaggcta atgactttcg taatttgtga gctttgctga tcttggtcat
     2221 tcatggtcat aggcacttct gttggaacaa tgggaaatta caaaaagttt taaattataa
     2281 actgggtagc tgcaacatac cagtatttca gtatttttat ttttcaattt cagcttgccc
     2341 atgtctttcc tttaataaat tttgtaaact gagccgttca gcttctgtta agccagtttt
     2401 tctggatttt tctatgtatt gtcgtatcaa tatctcacga ttttgttttt gtaaaaataa
     2461 taagacatca ataaattctt tagtcagctc ctgctcaggc acttgatgat cccaggctgc
     2521 caatttaact atcaattcaa aataactgct attgcgccag aattcaatga gggtggcagt
     2581 attggcattg ggcttgtctt ttaattgttg caataatttc aataaaatat gatgctcctg
     2641 ttcatggagc aaagcaggat tgatttgttg tattgctata gaataaattt cggggttttg
     2701 taagaggagc gcgatagcaa tacgcagtgg tgaacgagaa atagcagctt gtacttccgg
     2761 cttgctttcc gatttatcag taatcaaatt agttaaccga tggctttcaa tatgggttaa
     2821 gcgtgacagg tcatcaataa gcaactgttt gtatgagccc tcaaccattt tttgcaaata
     2881 gggtttcact gcgttgataa gctgtgtttt tcctgcaggg tttgacaaat taatgtcttt
     2941 acttaacgta tcaaagaaaa agcggtggag tggtgttgct tgttgcaaga gctcgaggaa
     3001 accgtttttt ccttcatttc ttaccaggct atccgggtca tgttcattcg gcaagaaaat
     3061 aaatccggca tctaatccgg cattcaaatg cggtaaactg ctctccaatg ctcgccaggc
     3121 tgcttgttta cccgctgcat caccatcaaa gcaaaaaatg agatgtttgg tatgctttgc
     3181 caatagttgt atatggaatg tgctggtggc agttcccaat gttgctacca cattaaaaat
     3241 gccatgttgt gccaaggcaa tgacatccat gtaaccttca acaacaataa tactgtcggg
     3301 atttttctgc tgagatagaa cttgatgtaa gccatataac tcacgacttt tttgaaaaag
     3361 tactgtttca ggcgagttaa ggtatttggg tttttgatct ttatcgagta cacgtccacc
     3421 aaaaccaatc acacgaccgt ttctgtcatg gatggggaac ataattcgat tacgatatct
     3481 atcataaatt ttaccttcat cgcttttaat gagcataccg gtactcaata gttcacgctg
     3541 attgtttggg aaagcttttt ccaggttatg ccaaccttca ggtgcatagc ctaaatgata
     3601 tcgtttggca atttccccac taagacctcg gttgcgaagg tagtcaattg ccacttgtcc
     3661 attatatttt aatttttttt ggtaatattg gctgacttct gacatgagat tatagagatt
     3721 gagagatgat ttatgtttct ctcctgtacc atcccgtggg acacttaaac ccaagcgtgt
     3781 cgcgagggtt tcaacagcat ctgtaaaacc ttgattcaga taattcatta caaaactgat
     3841 ggcgttacct gaagcgccac aaccaaaaca atgataaaat tgtttttttg caacgacatt
     3901 gaatgatggt gttttctcat tatggaaagg gcaacatgca atatgactgt tgcctctttt
     3961 tttcaaagga acatatccgt ctattaattc caccaaatca gtgcggttta atagatcatc
     4021 aatgaatggc tgtgggatta agccagacat attggaactc agcttaattt cgcctttatc
     4081 cgggcgctga cttgggccat gtccgcgcgt ccttgtaatt ttggcttaag gatagccatg
     4141 actttaccca tatcagacat tttttcagcg ccagtagact tgatcgcgtc gcagattaac
     4201 tcttcaattt cattgtcagt taaaggttca ggcaggtatt ttgaaatgat attcaattca
     4261 aactgctcct gggcaacgag atcgtcacgg cttgcttttt caaattgagc aatagattct
     4321 ttgcgttgtt tggacatttt atccaggatg actaacatgc gctcgtcatc cacttcaatg
     4381 cgctcatcaa cttcaatttg ttttaccgca gcactgatta agcgtaaagc attgagcaag
     4441 tttttgtcct ttgcgcgcat agcgtcttta atgtcattgt tgatttgttc ttttatagtc
     4501 attttaattc cttaaaaaac aaaaggtcac atacaatgtg accttggtgt aatataggac
     4561 gagctgcgat ttactaccga aactgccacg aaggctaatt tcaaataaaa acaattaaaa
     4621 ataacagagc ttccaaaata gggcactgtt taagtgcctc acgcaaactt gctttttagt
     4681 gcagtcaatc ttgaaaaaat cagatcaggt agtgcttttt ttacgtcggt gaccaacacc
     4741 tcgtcttgaa ctaacatcac gagatatttt tttaagatga cgttttactg cagcagcttg
     4801 cttgcgctta cgctctgcgg ttggtttttc ataaaactca cggcgacgta attcggttaa
     4861 aataccggct ttttcacaag agcgtttgaa acgtcgtagt gcatattctg ggttttcgcc
     4921 ttcttttaca cgaacggtag gcattaacta gtcctctggt tatttaatct taataggtgg
     4981 gcaattctag tgctagattt tccacacgtc aagattaatc tatacgtttc ttgctatttt
     5041 agcaaattat caagacattg ttctttaatt taatggtatc ttttgtctgt gatataattg
     5101 aaaaatgatt aaaatctgga aaattgattc cgattattga ggtagttcat gcttgtattg
     5161 ggtatagagt cttcctgtga tgaaacaggt gttgctgtct atgattcaga atcaggatta
     5221 ttgtcccacg ccttgcattc acaaattgcc actcatcgtg tacatggcgg tgttgttcca
     5281 gaactggcat caagagatca tgtgaattac ttggttcctt tggtcgatga ggtactaact
     5341 aaagcgcaaa ttcgtaaaaa ccaattggat ggaatagctt acaccgcagg acctggatta
     5401 attggcgctt tgttagttgg ctcctgcttt gccaaaagtc tcgcctacgc cttaaatatc
     5461 ccggcattgg ctattcatca tttggaagcc catctgttag ccgcaaaaat ggaaacccca
     5521 tctctggact ttccctttat tgccctgttg gtttccggtg gccattgtca actgatagaa
     5581 gtaaataata ttggtgagta tcgattatta ggagatactc tcgacgatgc agtaggagaa
     5641 gcctttgaca agacagccaa actgatgggg attccctatc ctggaggagc cgtgttggca
     5701 aatttggctg atcaatgttt atcaactccc tatcaatttc cccgtcccat gactgacagg
     5761 ccaggcttgg actttagttt tagcggacta aaaactcatg ccttaaacac ctggaatcaa
     5821 agtgaaaaaa aagaaagcga ccgctccgaa attgcaaaag cattccaaca agcagtagtt
     5881 gaaacactaa ttataaaatg caaaagagca attaaagagt ctcaatcaaa gcgccttgtg
     5941 gtagccggtg gagtaggagc caataaagcg ttgcgcagcg ctttgcaaaa atggattaag
     6001 gacatccaag gggaagtgta cttccccgct ctggaatatt gcacagataa tggtgcaatg
     6061 gtcgcttatg caggatgttt gcgcatgatg cggggggaga ttgatggggg gttgggggtt
     6121 atagtcaaac ctcgttggcc cctcgcttga acgcccgtct tcggcgcatc ttgccagatc
     6181 attgctctaa aaaatgctca tgtactcatc tatacactcc gcttttttag tcgcaatgat
     6241 ctggcaattt gcactcgaat atgggcgttc aagggtaggt gacttcggcg catctttgct
     6301 tgtgcattaa taggtgctgc gccttattcc ttttctttga ctgttttcgt tttaggtttg
     6361 gttgatttcg gttttttagt tgtttcttcc ttgctggttg tcttttttgc ttttgttgtt
     6421 gcttttttga cttttggttt ttttacagcc tcagcttgtt ctgtcttttc tgtttcatca
     6481 atattcgtat caatgacttc tttgccagga aactcttgct ctgcagaagt agctggagaa
     6541 gcctccatta tttcctctat cacactgtgt ttgaacttga ttttaggctc ttttccatct
     6601 atcaaacgat taaaattatc tctgtgtttg tagagaacca gaatggtgat catgaataat
     6661 ggggggaaaa tattaagatt acccaccaat attaaggagt aaaaaggagc taaagagata
     6721 gaagctattg atgctaaaga ggaatagcgc cagaaatttg ctaccagtaa ccaggtcgcg
     6781 gcaaccatga caccaattat aaaatgaaat gctaacaagg ctcctattgt tgtagcaaca
     6841 ccttttcccc ctctgaaatg aaagaaaaca gggtacatat gaccaacaac ggctgctaaa
     6901 gcagtaaagg caacggttac aggttcggca cttaaaaatt tggccaggat gacaggaagt
     6961 atacctttta aagcatcgaa taccatgacc ataataccgt actgcttccc tccaagacgg
     7021 agtacattag tagcccctgg gttttttgaa ccctcttctc ttgggtcggg gagcccgaac
     7081 gtacggcaca ctatgatagc tgaattaatg gagcccatta aataaccaac tagtattaga
     7141 aatataaaaa gcgccaccga tttctcctta ctcgggaata atttttatta tcaaccaatc
     7201 tcaattaaca ggcaatttta agatagaaaa tatgccaaga ctagataata gaattcattt
     7261 catgttattt caatttaatt ccatgtttag tgctggaatg agtgtaggaa ttgacttgtt
     7321 tatgcaaaca gcagccatgg ttggtataat ttgatccaac aagagcgttc atattgctaa
     7381 aaaagaggat gttccataga atttgaatta tttgcaatag tttgagacgg atactgaatg
     7441 ttgcttttta gtcctgttcg gtgtgtcact atcattctac tccattattt aaggaaattt
     7501 tactaaagct atggatgcca ttcatctcct ttctgcaaca gaaatcattc gccgcattaa
     7561 aatgaaagaa ctctcggctg cagaggtcat ggtggcgcac ttaaaccata ttgataagat
     7621 taatcctgta atcaatgcgc tcaccgaacg tattccccct gaggaatgct taaagcaggc
     7681 aaaggaaatt gacaagagta tctcttcgaa aaaaaatctg aacaagttaa tggggttgcc
     7741 tgttgccata aaagacgctt tgtatgtgaa aggactaatt tgttcttcgg catgttcagg
     7801 attttacaag ggagaaaaag cagtacgtga tgcgaccctg gtttctcgac tgaaaaagga
     7861 aggagcaatt attcttggct taactaatgt tcctgaattg tgtcgcggtg gtgattcgga
     7921 taatttaatc tatggacgga ccaataatcc ttatgatctg gcaagaacca gtggaggcag
     7981 tagtggtggt tccgcggctt tgatcgctgc aggaggcgtt ccttttgctt tgggctccga
     8041 tggtggaggt agtttaatgc agcctgccca ttgttgcggt attgttgcat tgaagccaac
     8101 acatggccat cttcctcata caggatctgt aggtggggat tcctatggtc ttattggcaa
     8161 cctgatttct tttggaccaa tggctcgttc tgtcagtgac cttcgtttag gactctccgt
     8221 gcttgcgggt tctgaccaat atgatcctta tacaaatccg gttcctgtta tgcctgctgc
     8281 accactaaaa aaattacgtg tagcctattt tacagagaat ggttttacgc cagttgatgc
     8341 ggagatacag aacgttgtta aatcagcagc cttggctttg caggatgatg tggctatagt
     8401 gagggaggta cgacctgatt gtgtcagcaa ggcttttgat ttgcactggg agctttttct
     8461 cgggggcgat cgcggagcgg gatttaaaac tatgttatcg gaattagggg ttaacaatct
     8521 gtcatgggaa ttacaagagt tcttgcgtca agcggaacaa acgcaattct cggtgactca
     8581 attgcatcaa cgcatgcgtg aaatcgattt gtttcgtctg gaactcgctt tattcatgca
     8641 agattatgat gtattgatta gtccagtgtt tccaacagta gcaaaaccgc atgggattgg
     8701 gattaaagaa atcagcgatt tttcctatgc gatgacacac aatttaagtg gatttccaac
     8761 gattagccta agatgcggga cttcagccga ggggttgccc attaatgtat taatagccgc
     8821 taatcgctgg aaagatacta cctctctggc tgttgcagaa cgcgttgagc aattaatggg
     8881 gggatataaa cccccttctc taatccactc tctgcaatga agcaagaatc aataattttt
     8941 gaaacagcga actagggcag aaacaaggct tgcaaatcat tagtatattg cctgcctaat
     9001 ggtgttactt gccaatgagt atctgttaaa ttaatcaatt gtttcttatt agctaatttc
     9061 agcaagggta aaagctgatt aagttccaat cctgtcgttg tcgtaaataa gtctaaaggt
     9121 atggattgtt ccaaccgggt tgtattcagc ataaactcaa acaacaattc tttacctgta
     9181 atggtttcgc aagcagccag ataaggtttc tcattatcca gataatcttt gggttgacgg
     9241 tgcttacggg ttcttattat tgcgtttggg gttgtcattt ttccatgagc accagcgcct
     9301 atacccaaat aatcaccgaa tagccaataa ttgatattgt gtctggcttg cttttccggt
     9361 ttggaaaagg cagaaatttc atagcggtga tatccggaat tatgtaacag ggcaaatcct
     9421 tgttcctcca gcaagtaatc ttcttcttca gaaggtaggg gaggagtatg cttgtaaaat
     9481 acagtattcg gttctatggt taattgatac caggataaat gctcaggttg ataggataaa
     9541 gcggtcttaa gatcctgtaa tccctgcgtt acactttgat tgggtaaact gtgcatgata
     9601 tcaagattga gattatcaaa gccggcttta cgggcagtat ctatagcaga atgggcttgt
     9661 ttctcgtcat gaatacggcc aagaattttt aaatgattgg gattaaagct ttgaattccc
     9721 aaagaaagac gattgattcc tgattgtcta taatcagtaa atcgatgttg ttctacagta
     9781 ccgggattgg cttccattgt aatttcaata ttttttgcaa aggggagtat gcgctttaat
     9841 tcattaaata agttgttata agcttcggct gaaaataaac tgggtgtgcc gccaccaata
     9901 aaaatggagt taatttcacg ggtattgtat gaggaaatat ccgttttaag gtcttcaatc
     9961 aaagcctgaa tatactggtg ttcgggtaaa acatcagggc ttttgtgtga gttaaaatca
    10021 cagtatgggc attttctgat gcaccaggga atatgaatgt acaaggataa cggaagcatt
    10081 acaattttta aactggcttg ttagggaatg cgatagtatc atgatagcaa ttttgctaca
    10141 aaattaagtg atgaccattg atgcgaaata aaccaaaatg taagcaaagg accgtgatag
    10201 ccaaatcccg gttgtttaca gttgaagaaa tgtatctgga attttccaat ggggctcagc
    10261 gagtctatga gcggattaaa agtcatggtc atggtgcggt gttaatagtt gcattgactc
    10321 ccggagaatc attattatta gttcgggaat acgccgcagg tactgattct tatgaattat
    10381 catttcccaa gggagtgata gacagaaatg aaacccctat agaagcagct aacagagagt
    10441 tacgtgaaga gactgggttt gcggcaaggg aactgcactg gattcgttcc atgtcattgg
    10501 ctcctggtta ttttggagct cgcattgagc ttattgttgc agagggtttg taccattcgc
    10561 cgttgccagg tgatgagcct gagcctcctg aaacagtaga gtggccttta cacgctgctt
    10621 atgagttatt ggaaaaaccg gattttaccg aggcgcgcag tattgctgcg ctatttttag
    10681 ttagagagtg gttgaataag aggaaatcat atgacaaata atcgagtaag agttgctgtc
    10741 acaggtgcag cagggcaaat aggttatgcc ttggtgtttc gtattgcttc cggtcaaatg
    10801 tttgggccaa atactgaagt ggaattgaat ttacttgaac ttgagccggc acttccctca
    10861 ctggaaggag tcgctatgga gcttgatgat tgcgcatttc ccttattaaa acgcattgtt
    10921 tgtactgcag atttgaataa ggcaatggat ggtgttaact gggcattatt agtcggttca
    10981 gttccgcgaa agcaaggcat ggagcgttct gatttattac aaatcaatgg tggtatattc
    11041 accaaacaag gtcaggcgat taatgattat gccagtgatg atgtacgggt atttgttgtg
    11101 ggaaaccctt gcaataccaa ttgcctaatt gctatgaatc acgcaaaaga cgtgccttct
    11161 gatcgttttt atgcaatgac cacattggat gagttaagag ctcgtactca attggctaaa
    11221 aaagcaggcg ttgacattac tgcagtgact caaatgacga tttggggtaa tcattccgcc
    11281 acccaatatc ccgattttta taacgcaaaa atcaatggaa cttctgctgc gcaagtcatt
    11341 aatgatgaaa cctggttaaa agagactttt gtatcaaccg tccagcaacg aggagccgca
    11401 gtgatcaagg cacggggttc atcttctgct gcttctgccg ccaatgcgat aattacagga
    11461 gttaatcatt tggttacgga tactcctgct ggcgaatcgt tttcaatgtg ccgtcgctca
    11521 aagggagagt acggagtcga tgaaggtttg attttctctt tcccatgccg aagagaacat
    11581 ggcgaattaa aagtggtcga gaacttggag ttcaacgatt ttggccgcga gagattcaat
    11641 accactttaa acgaattacg aagtgagcgt gacacggtca agtctttagg cttattggat
    11701 taaccagacc gggttaattc ttgatagatt tgattctgtt gtttttgctg attaccctgc
    11761 tttgtgaatt tttcagggca gggtgctgtc gcaccttggt tttataaacc aggtttcaat
    11821 gactcgtttg tttttaaatc agggtcttta ttgggatgtt gatgtctgtt tttatattcc
    11881 tcactcaaat acatttgaaa taatggcgaa ttctccagga tggttttagc gtctgtgttt
    11941 cgtaaaagag ttgatacgga acgaccattg gataatattt cgttgagagt atgaatcaat
    12001 tgctctattt gctcgaaggg ttcttttctt ttatgcatca aaacgggaat atgcttaatt
    12061 atagctctgg caagttgttc gtctgttaag taaagaatca agcgggtttg agaaattttg
    12121 atgaaatttc tttctgacct gagaatgata gtgtataaaa aatcaggatc tttttttaag
    12181 tgattgctca atttttggct aaatccaaac ctatcccggt tgtcaaccag cgtttgggtt
    12241 aattcagtat gatccaattg agcaaagggt tttcctacct ttttttcaag ccaggtcgtt
    12301 aaatcatagt catgatgaga atgattcagt gcgctgaatt gaaatcgtgg cgatactttt
    12361 ggatgagata agacctgatt ccagtcaata gttggcatgt ggtgcaattt agccagagca
    12421 tccatggatt ttctgattac attaaaatta atccgccatt tttcaatata aggcaaggct
    12481 tctctaattg ctctaagcag ttcaataaaa gtatttctgg cttttggatt gtagttctcg
    12541 gtggcttctt taagatgttg aactgctgga aaagtgtact tgccatttct cattgcctct
    12601 aaatgaaata ctgattgtaa tgattttagc aatacagaaa aagggtcttc cataccaata
    12661 ttcctccaca ggcccttctt taaaagtagt ttataaaaac ataaaagcca aatattttgc
    12721 aaaatggatt aataattttt attatgatta attttgttta tcgttttgat aaaaacaatt
    12781 gattttattt atttagcggt ttcacctaaa tttaacaatg aaaatggtgt taatttaatg
    12841 aggagaaaac ttatgattac ggtaggcaac aaatttccag agtttcaact taaagcaacg
    12901 gtggccaatg atgtaagtaa tgcatttcaa atgatctcta atgaaacgta tcggggtaaa
    12961 tggttagtcg tatttttttg gcctaaagat tttacctttg tctgccctac tgaaattgcg
    13021 gagtttggaa ggctaaattc cgagtttgcc gatcgagatg ctcaaattct gggtggaagc
    13081 acagacagtg aatttgtaca cttggcctgg agaaatcagc atccggactt aaacaatttg
    13141 ccatttccca tgctggctga tgttaaacga gaattgacag caaatttggg catattggat
    13201 gacgaggagg gagttgccca acgtgcgaca tttattgtag accctcaggg aattactcgt
    13261 tttgtgatgg ttacggattt aaatgtaggg cgcaatccag ctgaagtatt acgtgttctg
    13321 gatgctttac agaccgatga actttgtcca tgtaattgga aaaaaggtga agaaacaatt
    13381 catatcaaat aaattgaaaa aagaggcatc aatcatgtta gagaatgtta aagaaagtat
    13441 accggaagtg gctaaagaca tacgcttgaa tttgactaag gtacttgatc tggaacaaag
    13501 tgatggattg agtgagcatc agattttagg ttctgcattg gctgtgagtt attctttagg
    13561 caatgaggtt ctaattaaag gacttaaaga attggtgcat aacgaacaga ttgtacgtgc
    13621 agcacaactt gcggcaagcc ttatggcgat gactaatatt tactatcgct ttgttcattt
    13681 aactgaaatt aaggaaattg agcacattcc tgcaggatta cgaatgcaag gaatgatgac
    13741 tccgggggta gacagggtaa cctttgaaat attgtccctt gctgtttctg ctctcaatgg
    13801 ttgtggggct tgtgtcagct ctcacgcacg tcaattgaaa gagcacggct tatctgatac
    13861 aagccttgca cgagttggac gtattactgc tgtgattcat gcggttcatg tgagtggtcg
    13921 aatcaaataa atcattgcat ttgaagcaga ttatctgctt caaatgcaaa aaactattta
    13981 atgacgccgc acgctatacg gtcccccccg ccaccttgtg gaggattgtc actgtaagtg
    14041 tctccattgg catggatcat gacagctaaa ttgtgcatat cacttaattt caaacgaggg
    14101 gctaaagtag gtatcattgc tttcccatta cttgtgacat ataaaacagg taaatctccc
    14161 aaatgcccgt taccataagg gccttggtgg ctattggtat tttgaggatc ataatgtcct
    14221 tctgcatgca ttccgtggtt accacaatct gcagttttat gtaaatggaa accatgcgga
    14281 ccttcaggaa ggttagttaa gtctggtgta atcaaaacac cataaggggt ttgtgtgaag
    14341 gttaccttgc ctattgcaac tggtttggga ccagttgtgt aaattggtgc tgtaagatca
    14401 tcggcaatag ccatgcttga gaataaaata gtaccaatta atattattcc tgatttattc
    14461 atgtcatttc ctggtatttt tcaaagcccg ttaatatttt tagtattatc caagaaaaac
    14521 atttattcca caggatttaa aaaaaaatcg attttttggg gttaaaaata tttttaattc
    14581 agacatttag tatatcagga tttgcacgtt caaaccttca ttgtgtagac catgagagtt
    14641 tcgtaaactc tattttaaag tttagagaga ggattacaaa ttaattgaat cggtaaaaaa
    14701 tgattgctgt ataataagca aggtttatac tttatgtttt aagctttaat taaacagaga
    14761 gtcctcatgg aattgagaat tgataatacc ccttttaaag ccatttttca gcctttggat
    14821 ctgggcttta cccaaatcaa gaatcgttta ttgatgggtt caatgcacac tggacttgag
    14881 gaagataaag aaaatttaaa tcgcctggca agcttttata gagaaagggc tcagggtgga
    14941 gttggattaa tcgtaacggg tggttttgct cctaaccgag ctggccgtct ggctcccttc
    15001 gctgccaagc tgactaatag taaagagcaa catcggcatg aggtcattac tcaagcggtc
    15061 catgaagcag gtggaaaaat tgccttgcaa atattgcatg cagggcgtta tggttatcac
    15121 ccttttattg tagcaccaag cggcattaaa tcccccatta gcccgtttaa accgtgggaa
    15181 atgagtaaaa gacgaattat caaaaccatc aaacattttg cccgatgtgc cagactggct
    15241 caattggctg gctatgatgg ggttgagatc atgggaagcg aaggatattt gataaaccag
    15301 tttatagtta ctcataccaa tcatcgaagg gatgaatggg gaggaagttt ctccaatcgc
    15361 attcgatttc ctattgaaat agttcgcagt gttcgtgagg cggtaggcga gcactttatt
    15421 atcatttatc gcttgtccat gcttgattta gtggctgagg ggagtaattg gcaggaagtg
    15481 gttaccctcg ctaaagccat tgagcaagct ggtgccagtt taatcaatac gggtataggt
    15541 tggcatgaag caagaattcc aacgatagga acgatggttc catctgctgc cttcactcca
    15601 gtgacccagc atttaaagcc agaaattaaa atacctgtaa ttacatctaa ccgaattaat
    15661 acaccagaat tggctaatca attattagaa gatggggtcg cagatatgat ctctatggcc
    15721 agaccatttc tagcggatcc ctgctttgca gaaaaagcaa aattaggcga aagcaaagcg
    15781 attaatgtat gcatcgcttg taatcaagca tgtcttgatc aagtttttgt gaacaaaaca
    15841 gcatcttgcc tggttaatcc ccgcgcatgc aacgagacaa ccttggttta tgaaacaaca
    15901 aatcaaccga aagctattgc tgtagttggt tctgggccag ctggccttgc ttttgctgta
    15961 acagcagctg agcgagggca taaaattacc ctgtttgaaa aagcgaatca gattggcggg
    16021 cagtttaatt tagcgaaaaa aattcctgga aaagaagagt ttcagcatac cttgaattat
    16081 tttgcctacc aactccaaaa gtataaagtg gatgtacgtt tgaatactga agctactgtg
    16141 gaattactta aagactatga tgaagtggtc ttggccagtg gaattatacc cagagttccc
    16201 gaaattaaag gcatagatca taaaaaggtg gtgagttata ttgatgtgat tactcaaaac
    16261 aaacccgttg gctcacgtgt tgccgtcatt ggggcaggag gaattggttt tgatgtcgct
    16321 gagtttttaa ctcatgatca tcaattgact tctgagcaat tttataatga atggggaatt
    16381 gatattagcg ggcagcatag aggtggatta aaaccacctg aaattgcacc gagccctcgt
    16441 gaagtgtatt tattacaacg caaaaaagaa aaaatgggta aacggttggg aaaaactacc
    16501 ggatggattc atcgctccag tttaaaacat aaacaagtca aaatgctttc gggtgtaaat
    16561 tacgagcgta ttgatgatca gggattgcac ttgactattg atgacaagcc tcaagttttg
    16621 gaagtagatt ctatagtgat ttgtgcaggc caaatagaat taaagaattt gtatgagcct
    16681 ctgaaaatgg caggcaagtt ggttcattta ataggaggcg cttataaagc tttggagctt
    16741 gacgcacgtc atgctattga tcaggcttgt cggttggctg cattagtatg agtaaaattg
    16801 ctgtagtgat aaatcctata gcaggcggcg gtcgcggaaa aaaagtttgg aaagtcctaa
    16861 aacccgggtt acatgcccta ttcgaacaaa ctgtttacag aatatctaac agggttaatg
    16921 atcttgaagc aatcacagac ggacttcttg ctgaaaaccc tgacagcctg ttaataatcg
    16981 gtggagatgg cactttaaat catgttttga atggcttaat tgagaaagat aaattaaaaa
    17041 atccgcaaac aaaaatcgct ttttttaacg caggttgtgg cggtgatttt acaagacaat
    17101 ttccccaaca gaaaatgacc gaattcctgg atagattaag tcataaccaa tttatcaagt
    17161 gtgatattgg gaaaattact cttgccaatc aattgtcgag gtattttatt aatattgcca
    17221 gctgtggtct gtcaggacat gtggttttgc gtgtctctaa aagcaagtgg ttaaaaaaac
    17281 tgggcggtac tctgaattac ttgttgcatg ctttaaccgg gttgatgacc tatcgtaaaa
    17341 ccaaagtaag ggttcagata gacgatgctc taccatttga tagctccatc ctgctcatgg
    17401 ccgtctgcag tggccagtat tttggcggag gtatgcatgt agcgccaatg gcaaaagtga
    17461 acgatggctt gtttgatgtg gtttctttta atgaattttc aaaactaaat gcacttttaa
    17521 aattatataa aatttattca ggagggcatt tgttggataa taatgtccat tatgttcaga
    17581 ctaaaaaaat taaaatagag ccgttggaag aaagtaaaat agaaattgag gcagatggtg
    17641 aaattattgg ttgcttaccg gcacagtttg agttaattaa agaaactctt cctcttatta
    17701 tttaaaaata aaggtcaaaa tagggcactt tctataaaat tattttttat caagacaggt
    17761 tgttgcctca agacgagatc acatcattaa gtgcaataaa caacacaaac gattgttcat
    17821 attttgtcat tctgcgcagg cgggaagcca tttttattgt aagctgtgga gcaaaatttg
    17881 aaatagattt tcacttgcgt gggaatgaca catgtgatgt attatttagt caaaatatga
    17941 ttttgccctg gttgtagtct tgataaaatt aataaatata gtgcatcatc ttgttaattt
    18001 tgttattata atgcacattt tttatattag ttagcctttt cagaaagacg ctttattata
    18061 ttcaggaaca gtatgaatca agaaatatca aacttctcta cttttaattt ttctaatgct
    18121 ttaaataagg cgttagaaga tatgaagttt attacgccat cgcctatcca ggctcaaacc
    18181 attcctttga tattgcaagg tcgtgatgcc attgctcttg cgcaaactgg tacaggtaaa
    18241 acagcggctt ttgcattacc aattttgcaa aatttgtccc ctgagatatc taccactcag
    18301 gctttgattt tagcccctac tcgtgaattg gcaatccagg ttgcagagca atttgaatta
    18361 ttaagcaagt accaacgcaa tgtgacgatc gccgttcttt gcggtgggca agaatatggt
    18421 cgtcaattaa aacaattgcg tagtggtgcg caagtggttg ttggaacgcc aggacgtatt
    18481 ctggaccata tcgataaagg caccttgctc ttaaataatc tgaagacatt tattctggat
    18541 gaagcagatg aaatgttgcg tatgggtttc atcgaagatg tagaaaccat tttagaaaaa
    18601 ctgcctgaaa aaaagcaaat ggcattgttt tcagcaacca tgccctatcg aattcgtcaa
    18661 atagccaata cttatttgaa tgaccctgcc tcaattgaaa tccgtatgga aactgcgacg
    18721 gtcaagtcta ttgagcaacg gtttttattt gcttccgtac atcaaaaacc ggatgcgtta
    18781 atccgggtct tggaagtgga ggattatcaa ggggttatcg tttttgtacg cactaaaagc
    18841 agtactgagg aagtagcaga actattgcaa caacatggat taagagcaat ggcaattcat
    18901 ggtgatatta ctcagtcttt gcgtgagcgg attattgctc aatttaaaca aggagcgatt
    18961 gatattctgg tggcgacgga tgtcgctgca agaggtttgg atgtcgagcg agttactcat
    19021 gtgattaatt atgatatgcc acatgataat gaaacttatg ttcacagaat tggcagaaca
    19081 gggagagctg gccgttctgg tgttacgatt ttatttgtaa ctcccaagga atcgcgttta
    19141 atttccagca ttgaacgcca taccaggcaa cgaattgaga aagttcaagt tcctaatgac
    19201 catatgatac agatggctcg tcaacaaaga ttcatgtcta acattactac tcgactgaat
    19261 catgagcatg tgtattccta caaaaggatt gtggaagaat acattaaaga gcatgacgta
    19321 tcagcagtag atgttgcggc tgctcttgct ttattgctgc ataaagacat gccttggaga
    19381 aaagaattaa atatgcctaa agcagcttca gcattcaaag aagagagagc tcgggcagga
    19441 gaaaaaggat tggatttacg tggtcgattt aaggaagata gaaaaagttc ttttgctgat
    19501 cggaaaggtg cgcgaggaag caagaaagat tttgccaaag acttttctaa tgaaagaaaa
    19561 aaatttaatg atggcaggaa ggccgttaat caagaatcag tagcccagga tttatttcgt
    19621 atcgatgtag gcaaaattca tggagtcaaa cctggcaaca tagtaggggc tattgctaat
    19681 gaggctggct tgcaaagccg atatattact ggtcttaaaa ttcatgaaga tcactctact
    19741 gttcgtttac caaaaggtat gccaaaagaa gtgatgaagg acttaaccaa ggcttgggtt
    19801 tgtggccgcc aactcaattt aacattaatt ggtagtgcct gaatagctta gcagtaaagt
    19861 gctgttagcg ctatcaattg gcctttaatt attttgtaaa acgatttcgc ctgtcagggc
    19921 gagatcataa cattaagtac aataaatagc gcaaacaatg gttcacattt tattatcccc
    19981 gcacaggcag taagctatct cttctctata gcaaagggag gggtaagatt tgaaacagat
    20041 tcccactcgc gtaggaatga tatgcatgac gtattattta atcaaagtat gattttaccc
    20101 tgaagaccta cctttttcct ggcttgtttt tctaatttaa tacctctaat atggattggt
    20161 gttctgaaat ttaacactga ggggccaaat tatgaaaaag aaacatgaac ccggtttttt
    20221 cggtagagaa accaagaata tcaaagacat tgaaagcaat agatacgatc ctgattgccc
    20281 aattcggaga gctgataagt ctcgtggatt cagccaaaaa gcaagcagcc agtttcagct
    20341 acgaataaaa gaagaagtaa aagaagcgtt agacgacatt cgtaatgaat ctgcctataa
    20401 atttaattaa ccattatttt atctctgagt gtaagagatc agctccttct tgaaacgacc
    20461 gtatttggtc catcttgaac ggaaataacc attggagtac tgatgatctg tatatttctg
    20521 agtttaatga agcctgacaa gaggcttcat tagtaataaa acactacctt tatggtttgt
    20581 tgccaaacaa cactgacgca gcagtagctg ctacggctac agtggcagca acgaatggga
    20641 aaagaacata ggggttttca aactgacttt tataaggtac ttcgtctttg agtttggcaa
    20701 ttaattcttt tcttatctga ctactatttt ctctcacaga ttttaatcta ttctcagggt
    20761 cggtgttcgc tgtaagtaat ggaacatagt tatccaataa tatatttcgg gcactttgta
    20821 tttgctctgt agtctcactg gttggatttt catatttctc cgaaacaaga gctatggctt
    20881 ctgcaattgc agaagtaaat tttagcttat cctctttgga ccagtcatca gcatttgtta
    20941 tacgtgaatt aatttgatag cgggggtttt catattggcc ttgagtaaaa tggtgaattg
    21001 catcatccag cttcttgtcc acagagagta tggtttcaag aaacatcaag ctattaatca
    21061 ggaaatttct ttcaaatcta gtcattatta tggttctcat aaagaaataa gggctgacga
    21121 catttctgct gaatcgattg caagatattg attttctgta ccctgttaca aaagaaagca
    21181 ttatgaatcg agtcagggaa gtgtttacag gatttactat aattaggttt ctgttttaga
    21241 tcaacaaaaa atttatatac agtaatgtgt taaatgccgt ggaaaaattg ataaatcatt
    21301 atataattga atgaacctaa agatataaaa tcaatctatt atttaagggg tttcatgaaa
    21361 ctattagcat ctttgtgtgc ttttttattg tcgggagctg ttagcgcgac accacttaat
    21421 aacatagttg tatttggtga tagtttgtcg gataacggta acttatatga atacatgaaa
    21481 caccaacttc ctcaatcccc cccttatttt gaaggtcgtt tttccaatgg cccagtatgg
    21541 atagaacgtc tggcagcttc atacttccca aatgatccca actctcattt gctggattat
    21601 gcttttggcg gagcaggagt ctctgtcgat gaagaagatg atgaagtatt ttttacctta
    21661 agaagagaag tgaattctta tttgttagca catcaggata aagcaagccc tgacagtctt
    21721 tttgttattt ggataggggc aaataattac ctcggaatgc ctgttgaagt ggacgaaacg
    21781 cttaaaaatg ttaaccgagg gattacggat agcatacaac gtttggtaga caagggagcg
    21841 aagcatattt tagttctcaa tttacctgat ttgggaagaa ctcctgccgc tctggagttt
    21901 ggctctgttg aagagatgac ctatttctca acacagcata ataatgcatt atccaataca
    21961 gtcgattatt ttaaaaaaac ttatcctgaa gttgagtggt tgttttttga cacgggtagt
    22021 cattttgacc atgtgataga acatgctgca gagtatggat ttacaaatat aacaggtacc
    22081 tgctcctttt cgatagtaga tgagattact aaaaactcgg ttttgaagat ggttgcgtct
    22141 gtgaagccag aattgacaga aagcgcatgt gatggttatt tgttttttga tttggttcat
    22201 ccgacagcgt tggcacataa aataatggca gaaaaagcgc gtttgatgct ggatgaagct
    22261 ggtgtggagt tcgccgagaa ttaagagttt tttatggtga tagcaaatgc aacttgcatc
    22321 gtcattcaaa tgaatccgca atgataagaa cggggatttt atactgagca gaacccaatg
    22381 agagaatgat gtgtttgaac ttcgcaaata ttctctcatt ctaactggca ctaactagtg
    22441 cgtcttgtcc atcataggca attttatagg gaaatatgaa tcaaattaaa tggatagtac
    22501 tactattttg ctttgttgtt tccaatgtat ttgcggataa gcttgacata accgtcgatc
    22561 aaaaaaaaat tatattgcct tattggctat caaggcctgt ttcctatggg gcggttatca
    22621 ttgttagtgg tggagagaca gcgcaatggt ccttattatt ggaacagttt gccaagggat
    22681 tggctcgaaa tggctggtca gtcgtattat tgaattgtac taaaaataac tcgataccct
    22741 ggattaacca attaccggaa gtcatcagta ctttaaggca gaataataat aaaagaatcg
    22801 ttttagtcca ttatggtgat caacttaatc tggcattaga gtattttagc aagccccaat
    22861 ccaagatgat caatgggcta gtgatgttgt ctgcctatga tttaaataga aatttgcaaa
    22921 aagtacctcg gttgcgtttt ccactgtttg atattgtggg gcagtttgat tatgacatgg
    22981 taaggcagca acgtaagagc agggaagaaa aatttaaaga gaatagttat ttagccattg
    23041 atatacctgg tgctactcat gactaccaat acagtcaaca attattattg gcgtttgtcc
    23101 atggttggat ggcaaagctt ccagagtttg agccacagcc accgccgata ttggtttctt
    23161 atctggaacc cgtttattct acagcaagtt caatcgtatc gaccaacaaa ttggactacc
    23221 aacccttggt ttcacagatc aattcatagg ctttcataat ttgatgagtt ttatcgttag
    23281 ccaatttaat catttcttcg ggtaaacctt gagcaattaa tttatctgga tggttacggc
    23341 tcaatagacg tctgtacgct cttctaacct cctgtttgtt tgcattcgga cttacctcaa
    23401 gtaatgcaaa agcatgggct aaagtattag ggggtgattg aggtttgtaa ccctgttgac
    23461 cttgagatga ggaggtatgc ttgtactcct gttgattatg atagtgttgt ttggattgtt
    23521 cttgctggaa ataagaacca aaatcctcat aaaaacgata ctgtttgtgt aggggagcaa
    23581 atcccagatg cgtaaaaatg ttatctagtg cgtgaatttt ttgactactt aatccatcta
    23641 cttgagctgc cctatactga atatctacaa aaagctttaa caaatcacgg ttatccttgc
    23701 agattctttg taattggata agagcaagac tgacattgaa atcagcttgt ttcccttcat
    23761 taaataatcg ttttgccaaa tccttttgcc ccttgctcaa tttcatttca ttcatgatgg
    23821 atttagccat gctaatctct tgttcagaaa cacggccatc cgatttcgca acatgcccca
    23881 ttatggagaa agtggcttca aagaaagctt tttgaacgat tctttgtttt tctgcatggt
    23941 acagccaatg gggattagag taataactga caaggccccg atcaaaaaag tttccaacga
    24001 gaatgccaaa caaagcgcct actggcccag cagttaagta gccaaagaag gcaccaagta
    24061 tttttcccca ccaggttgtt attacaaaga aatcgcgtaa gttcattatc agaaattacc
    24121 tataaaga
//
LOCUS       DACTJE010000032        23954 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.032, whole genome shotgun sequence.
ACCESSION   DACTJE010000032 DACTJE010000000
VERSION     DACTJE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 23954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23954
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.032"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..691)
                     /locus_tag="I9760_000992"
     CDS             complement(<1..691)
                     /locus_tag="I9760_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfhydrogenase subunit delta"
                     /protein_id="HAT5042769.1"
                     /translation="MKPRIAVYKFTSCDGCQLAFLNAGESLLTLSDLVEIVHFAEAGM
                     VDVDMKVDIAFVEGSISTPDEEHRIRKIRENSQYLITIGACATAGGIQALRNFADSRE
                     WMASVYASPDYIKTLTTSTAIAHHVKVDWELWGCPVNSGQILEAIRFLLSNATPKIKR
                     DSECMECKRKGNVCVLVTKKQPCMGPVTQTGCGSLCPTLGRACYACYGPKENANPNSL
                     GEWFLSIGLTKE"
     gene            complement(709..1554)
                     /locus_tag="I9760_000993"
     CDS             complement(709..1554)
                     /locus_tag="I9760_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit gamma"
                     /protein_id="HAT5042770.1"
                     /translation="MNEVVYDPYLPKEVEIIQRTQESSSIFTLHFRFTDEEHHKQFQF
                     YPGQFNMLYLYGVGEVAISIVSDPRKKTFLSHTIRAVGRVTKAMQKLQVGDRLGIRGP
                     FGVGWPLQKTIGKDIIVLTGGLGCAPSVSIINYILGRRRHYGKLSILQGVKHSEDFIF
                     RKQYAKWQKSDHTEVYIAADQAGPKWPWGVGYVTDLIDHIIIQPDNSVVMMCGPEMMM
                     NTAVKVFTQKGILENDIYLSMERNMECGIGHCGHCQYGGLFVCKDGPVFAYSQVKELF
                     KESGF"
     gene            complement(1547..2686)
                     /locus_tag="I9760_000994"
     CDS             complement(1547..2686)
                     /locus_tag="I9760_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit A"
                     /protein_id="HAT5042771.1"
                     /translation="MNDKRNCFLPHDKLQHLIDSLQNAGYSCVGPQVRDGAIVYDVLT
                     RAEQLPWGMRDHQEPGGYRLEQIPERKAFAFANGPQGIKPLLFKPQETVWRVERDEQG
                     KLCFKPHQPDETPVAIIGSRACDLAAMSIQDKVFIENNKPDPRYKRRREQLFVVAVNC
                     TYSSANCFCVSAGTGPAVNNSFDILMTEIEDGFVVEAGSDKGKKIMNQLDLHDAKSAQ
                     CTDAQNNVKQAAALQTKRIPLDNKRDLRDLLFSNLNHTRWDDVAERCLSCGNCTLVCP
                     TCFCHSEVEKPGLDGCTSEHHREWDSCFTTGHTYLNGKIIRDDTKKQYRQWLTHKVGS
                     WFDQFDTSGCVGCGRCVTWCPVGIDITEELAAISGESNARKIENE"
     gene            complement(2722..3780)
                     /gene="hypE"
                     /locus_tag="I9760_000995"
     CDS             complement(2722..3780)
                     /gene="hypE"
                     /locus_tag="I9760_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HAT5042772.1"
                     /translation="MNEILITKKLTGPKLNFKEGTINLTHGSGGRAMAQLIEQLFLTA
                     FDNEWLAQKNDQACFIVPSGRMVMATDAHVISPLFFPGGDIGSLSVHGTVNDVAMSGA
                     KPLYLTASFILEEGFPLKDLRKIVYSMADAARQAGVAIIAGDTKVVERGKGDGIFITT
                     TGVGIVPAGIHISGDAAKPGDKIIVSGFIGDHGVAIMAHRNNLEFQTKVQSDTASLHG
                     LVGEMVKAVPELHCLRDPTRGGLATTLNELALQSKVGFVIDESKIPIRTEVASACELL
                     GLDPLYVANEGKLIAVCPSEKAERLLTAMKAHPLGLHAEIIGEVIEDHRYFVQLKTKL
                     GGLRIVDWLAGEQLPRIC"
     gene            complement(3777..4883)
                     /gene="hypD"
                     /locus_tag="I9760_000996"
     CDS             complement(3777..4883)
                     /gene="hypD"
                     /locus_tag="I9760_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAT5042773.1"
                     /translation="MKYIEEFRNGAYAQAIINEIIATAEPQRHYQFMEFCGGHTHAIH
                     RYGIPSLLPNNVEMIHGPGCPVCVLPIARIDKAIALASMPQVILCSYADMLRVPGSGQ
                     KSLLTAKACGADVRMIYSANDALKIAQENPDKDVVFFAIGFETTTPPTAFVIKQAKKL
                     NITNFSVFCNHVLTPIAMDSLLQANEVALDGFVGPAHVSIVIGSFPYEQVCKKYQKPI
                     VISGFEPLDVLHSILMLIQQLNEGRCDVEIQYTRAVNRKGNLLSQRIMAEVLEIRDRF
                     EWRGLGFIPSSALKIKESFSEFDAEQRFELPESAAQEHKQCLCGEVLRGVKKPTDCKL
                     FAKACLPENPLGSCMVSSEGACAAVYAYGRVNAL"
     gene            complement(4880..5107)
                     /locus_tag="I9760_000997"
     CDS             complement(4880..5107)
                     /locus_tag="I9760_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HAT5042774.1"
                     /translation="MCLALPAQIDQVLEDNKAIVNLGGITKEISTVLLEQVSEGEYVI
                     IHAGFALTKLDEREANKTLSLFAHMLKDGLK"
     gene            complement(5117..7342)
                     /gene="hypF"
                     /locus_tag="I9760_000998"
     CDS             complement(5117..7342)
                     /gene="hypF"
                     /locus_tag="I9760_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAT5042775.1"
                     /translation="MELERVQISIQGLVQGVGFRPCVYKLAKQMELTGFVQNNASGVL
                     IEIQGNYLSQFIPKLREQLPPLAAIHNLEFTSLPIVPNERAFEILDSQQGKINTVITP
                     DVCICSECLGELFDPQSRYYRYPFLNCTHCGPRFTITRNLPYDRCQTSMSQFPLCQSC
                     QSDYLNPDNRRYHAQPTACSECGPQLALSVDKVAQRIRDGEIIALKGLGGYQLICDAR
                     NEDAISQLRLRKSREAKPFALMVVNAKSAERIAEVSPQARVVLESRERPIVLLRKKKA
                     LLPDEIAPGLNHFGIMLPSTPLHYLLFNSFLGNPDYCQWLDEYHSMVLVVTSANIGGE
                     PLIIDDKKAEQGLISIADKIVSHDREIITRTDDSVVRMINDTSVLIRRARGYVPAPLR
                     LPYEIPPTVALGGHLKNTFCITRGEEAFVSQHIGSLNNKATIEYFHESLNHLLKFLAV
                     KPERIAHDWHPDFYTSRLAQKYDISAYGVQHHHAHLASVVAEHGIQEPVLGLVLDGYG
                     FGSDGEAWGGELLLLEKAEFKRVGHFFPIPQPGGELAAREPWRMAAGILHTLGRGDEI
                     SQRFKEQPQSPFLLQLLEKKIHCPLTTSCGRLFDAVSALLGVESLSLFEGHAAMRLES
                     MVTHPQVLNKGWTINGNVFDMRPTLQFIANTTEQQLGANLFHGTLIAGLSEWVTGICR
                     EKGIHMLLLSGGCFLNQVLAEGLINSLIKSGIKPLLPKLLPPNDGGIALGQAWIVGIK
                     E"
     gene            complement(7358..8116)
                     /gene="hypB"
                     /locus_tag="I9760_000999"
     CDS             complement(7358..8116)
                     /gene="hypB"
                     /locus_tag="I9760_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAT5042776.1"
                     /translation="MCGICGCSVEKGQTMDNGHHAAHHEHSVPHEHSVDIEQNILAQN
                     NEFAAHNRDYFLRHKILALNLMSSPGSGKTTLLSKTISELGKEMDIAVIVGDQQTNCD
                     AQMLKSHGGDAWQINTGKVCHLDAHMISHAIQDISLKKQSLLFIENVGNLVCPASFHL
                     GEQFRIVVLSVTEGENKPLKYPDMFHSADLLLITKADLLPHVNFDLDLCKEYVRRTNP
                     SIDIITLSAVNGVGLKDWHDWLLHSFRLNVQSSS"
     gene            complement(8116..8457)
                     /gene="hypA"
                     /locus_tag="I9760_001000"
     CDS             complement(8116..8457)
                     /gene="hypA"
                     /locus_tag="I9760_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAT5042777.1"
                     /translation="MHELWLCKRIVEIIKQQATGNKCRKVKKIVLEIGQLVAVDKHAL
                     NFSFKVITQGTIAQNAELSIVEIPGEAICNSCQQIVPMKQYYDECLVCGNHSLTLTKG
                     EELKVKSMVVE"
     gene            complement(8469..9146)
                     /locus_tag="I9760_001001"
     CDS             complement(8469..9146)
                     /locus_tag="I9760_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HAT5042778.1"
                     /translation="MNDSGWFLISTALLLGMRHGFDLDHLATIDAITRTVRYKPSLSR
                     MVGFLFSLGHGLVVIIVSLIIGGGIISASTPEWLNEVGNIVSVTCLLLFGFLTLWNTV
                     HYSSSAIIPTNLRSYLFRKLADNNVHPLMILFIGALFALSFDTMSQIVLFSLAASALS
                     GFLFSGLLGFVFMLGMMMSDGLNGFFVASLIRRADGFSLLFSRLVGFGIAFFSIGIAI
                     INCCKLL"
     gene            9292..10251
                     /locus_tag="I9760_001002"
     CDS             9292..10251
                     /locus_tag="I9760_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAT5042779.1"
                     /translation="MSKTAKHKKLISCVVPVFNEEVLIAEFIAALDKTLKSITYPYEI
                     LLIDDGSQDNTFAIIQTLRKEYSLRCIRFSRNFGKEKALSAGLDHARGDAVILLDSDF
                     QHPLELLSEFIAKWEDGYDMVYAVRQNRSDESWLKRVCAKIFYHLTSKINRINIPANA
                     GDFRLLDRKIVNALQTLPERNRFMKGLYSWVGFKQIAVPFNVHPRKSGSSQWSFYSLL
                     DLAITGITSFTAFPLRMISLGGMVVAALALIYAAWIVLSTLIFGIETPGWATIVTAIS
                     FFGGLQLFAIGVVGEYIGRVFDEVKHRPHYIIDEESSFDDHCS"
     gene            10235..10624
                     /locus_tag="I9760_001003"
     CDS             10235..10624
                     /locus_tag="I9760_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAT5042780.1"
                     /translation="MTTALKLSHRLIYFTGVGICAAIVHLLIVFNLVNFLNLQPLLSN
                     AIAFLIAFNVSYLGHKYFTFNQLQDKKQLSLPHFFLVAASAGLLNETLYYLLLRFTTL
                     YYIAALVFVLSIVAVYNYLFSRFWACR"
     gene            complement(10601..11413)
                     /locus_tag="I9760_001004"
     CDS             complement(10601..11413)
                     /locus_tag="I9760_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HAT5042781.1"
                     /translation="MSDLKNIFLCADDFGLNHGITQGILKMARLQRLSAVSCIVNASS
                     FDVDAPDLLALKNGVRTGLHFNLTEGYFLSHPDKLCFGLRELLIRSHLGILNSRLISD
                     ELNTQLDHFIKVMGKLPDFIDGHQHIHQFPGIRRIIIDMYEQRLKGKSIFIRSTYPSV
                     NMSGYQAKTKILGITGGKILSSQLKKTNIPHNPYFAGIYDFSARTNYRSLFRQWLNNV
                     PTNTLIMCHPGEGDNSDIIAAARARELNYFLSDEFMQDCEEYQVNLTTGPKS"
     gene            complement(11580..12470)
                     /locus_tag="I9760_001005"
     CDS             complement(11580..12470)
                     /locus_tag="I9760_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAT5042782.1"
                     /translation="MPFAHLLLALLVVFVWGINFIFVKLSLEEFSPLLLCAVRFLLAS
                     VPAVFFIKPPAVPFKIIASYGLIMFALQFALLFIGLRVGMTPGVASLLMQVQVFFSMF
                     FAIIFLGEQPQVGQIIGALIAFIGIGVVALHFDHNVSLMGFLCILAAAASWGIGNLIT
                     KKIHSVNLIAVIVWSSFVACLPMFILSLVFEGPESFVTAYEHMTWKGILSVLYIVYIS
                     TWVGYGVWNWLISRYPVGMVVPFTLLVPVVGILSSVLILGEPFYLWKLVAGLLVISGL
                     CINLLTTRLFVAKTQQPETA"
     gene            12735..14081
                     /gene="sdbB"
                     /locus_tag="I9760_001006"
     CDS             12735..14081
                     /gene="sdbB"
                     /locus_tag="I9760_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbB"
                     /protein_id="HAT5042783.1"
                     /translation="MAKTITKDSFFHSRLQQFVANSLFPAGAGDWYKNKGYGKKGEVD
                     DEAPFRDFVEKQKTDKKSHYYKEFQGLDLSLKKVKTKLVSGESCQLEVMKCQPESNEP
                     KKPGTGKHIVYFPGANTYYQACFRDISTACKETGATVHAFNFPGTGLSSGKVREANDL
                     INAGISVVSSLIKQGVHPDDIILQGDCYGASIALEVKKQLEDQADIKVRAIMNNVFKS
                     FKAAVCDMITQSPWLPNILKSIVKRLLEFTGWHVTPGKKYKHADPYQCHIQHLGDQTL
                     ESSTLSGKVSKYHHEIQTGQTKSQKRAPITDTCPEEYPKDRDELDRKHYVRVKESAKE
                     RLASKFGVDKFGRVNAHFADLCELEMLDGQSVYQGFVNDYIARSNAYIEKHPQKGIKE
                     VQDDLRKLHYLQPADSIEITEDEAQDFNTVVDLITEEQQLRHDRFNDNTIGKSISM"
     gene            complement(14108..14665)
                     /locus_tag="I9760_001007"
     CDS             complement(14108..14665)
                     /locus_tag="I9760_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAT5042784.1"
                     /translation="MRILEKNIKKTPWYLKPFFWNQKRKYGQILEPALIWARIPRLFL
                     AVAAMYGVIDRKKSPLSPILRSLITVRVSQINWCAFCVDINSATLIKRAGSEARYKQL
                     AKWRSSSLFSPEEKAALDYAEKVTYSYQRPDDECFNQLKLYYSEQEIIELTALIAFQN
                     MSSKFNSALGVEAQGFCSRQLTGKS"
     gene            14714..15298
                     /gene="rdgB"
                     /locus_tag="I9760_001008"
     CDS             14714..15298
                     /gene="rdgB"
                     /locus_tag="I9760_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HAT5042785.1"
                     /translation="MKEIILATSNPGKIKELEQLLAPTICIPQADLGISDAEETGLSF
                     IENAILKARHASSLANKPALADDSGLVVPSLNGEPGIYSARYAGIKANDEDNIQQLLS
                     KMADLSQEQRQAYFFCAIALMRHAKDPTPLIATGVFHGLISVKPSGTNGFGYDPVFYL
                     NEYQCTAAELPAKIKNRISHRAKALNQLRALLPD"
     gene            15300..17015
                     /locus_tag="I9760_001009"
     CDS             15300..17015
                     /locus_tag="I9760_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAT5042786.1"
                     /translation="MNSEVDTLFAQAYKLQYEGHLPQAISLYEQILAQSPKHTETLHF
                     LGLTYAQLGDMENAILYFLQARKINPNDVGILNNLANAYKKAGQLDEAIKYYQQAIEI
                     KPEYVQAHNNLAATYALLNNYQKALHHYVIAVNTEPDFSAAHFNLGLLLLKNQQLSAA
                     KTQFNNVIALNPQHREAQFYLGILHLEDNLLSEAEQAFHKVLEQDHEHVQSLINLGVI
                     ALKREQNQLAVDYFTKALALDNEDIDARNNLAATFMHHDRFENALMHYDVLLKKEPDN
                     LEYLYNSGVAQMALGHLNEATLLFDQILTLQSDHTPSLNNLAAIYLKMDMRETAREYL
                     ERALAINPDDVISRHMLNAITGATSVNTTEKYAQNLFNNYALYYDQHMQGELHYKIPH
                     HIGRLIHQLQLLQTSHSLDLGCGTGLTGIVLREISKHLTGVDIAEKMIARAKEKNIYD
                     LLVCSELIDFLRKDKNDYDLAVAADVLPYFGNLDDIFNSINQHLKQEGYFIFTTEIST
                     TTPWKLESSARFSHQPEYIESLINKYQFHLIKQEKIPGRTQNKQVLEVMLYCIQKSTS
                     CFVSN"
     gene            complement(17333..18721)
                     /locus_tag="I9760_001010"
     CDS             complement(17333..18721)
                     /locus_tag="I9760_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAT5042787.1"
                     /translation="MTYQQKQIPRSVFRAYDIRGIIGKELDENSFYNIGLAIARYLQK
                     LNRRQIFVARDGRLTSHALATALKEGLLDSGIDILDLGAVATPVMYYATHTQGIDSGL
                     MVTGSHNPADYNGIKLVLAGKTLIQDEIDTLYALLGEVKQSAVRGTETAFDVIEGYIQ
                     RIVSDIQLKRPLKVVVDCGNGVAGPIIPRAISKLGCEVIPLYCEVDGRFPNHHPDPSI
                     EANLVDLKAAVANHQADIGLGFDGDADRLGLVTNKGEVIWPDRLMMFYSREILSRNPG
                     ATVVFDVKCSSHLEKEIQAAGGVARMCPTGHSIVKAVMKKEQAILAGEMSGHLFFKDR
                     WYGFDDALYSACRLLEIISFSHLTVSEQFELIPNSVNTPEIKIAITDEEKFDFIQRFS
                     EQADFPEGRILNIDGLRVEFPNGWGLLRASNTTPCLVARFEATDKDHLEQIQYLFKKQ
                     IQRLDSELDLPF"
     gene            complement(18722..19180)
                     /gene="dut"
                     /locus_tag="I9760_001011"
     CDS             complement(18722..19180)
                     /gene="dut"
                     /locus_tag="I9760_001011"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAT5042788.1"
                     /translation="MHQVIQLKILDSRIGDTIPLPAYATDGSAGLDLRVCISEPMQVA
                     PQQTVLLPTGIAIYIADPKLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGELKISCW
                     NRSQEHFTVNPGDRIAQLVFIPVVQASFEVVNEFTESSRGEGGFGSSGRY"
     gene            complement(19207..20403)
                     /gene="coaBC"
                     /locus_tag="I9760_001012"
     CDS             complement(19207..20403)
                     /gene="coaBC"
                     /locus_tag="I9760_001012"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAT5042789.1"
                     /translation="MQDFINKKILLGVCGGIAAYKSAHLVRELTRLGADVRVVMTHSA
                     QQFINPLLMQALSGNEVRIDLFDTQAERAMGHIELARWADYLLIAPASANCLAKMAQG
                     LADDLLSTLYLVTETPVIVCPAMNRSMWAHPATKANCELLKERGVFFVGPEEGSQACG
                     EHGLGRVSEADQIVNTLRLLDVNQLLHGKRIVITAGPTRESIDPVRYLSNYSSGKMGF
                     AMAEAAAMAGAQVTLISGPCHLQTFSDIEVIRVESAQSMLEEVNRHVQQGDVFIGTAA
                     VSDYRVLNPASEKMKKNENSELTLQLVRNADILSEVAKSGKASFVVGFAAETTDVISY
                     AKEKMKNKKLDMIIANQVGNGMGFESDVNQVTVITKNKQIELPLTHKTRLAGQIIAIL
                     AASLQN"
     gene            20480..21163
                     /locus_tag="I9760_001013"
     CDS             20480..21163
                     /locus_tag="I9760_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAT5042790.1"
                     /translation="MMVAHTAQQLDLREKLLTNGVHSLSDIELLAVFISSGNNKKSCL
                     QLAYELTKHLGNLRNILNADLQSFKSIHGLGEVRYAQLQAAKEICHRSDFIHLQKEIR
                     LSNTQQTYAFLKKRLRDYKNETFAALFLDNQHRIIAYEELFSGTINTATVYPRPIVER
                     VLQLNAAALILAHNHPSGLSDASQQDLAITERIRDALDLVDARLLDHIVIGDNEVYSI
                     FAENKWVCN"
     gene            21199..21435
                     /locus_tag="I9760_001014"
     CDS             21199..21435
                     /locus_tag="I9760_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042791.1"
                     /translation="MLQPFITDFIKNSSFQFIFVMGLVSDITVAGTIKHIVVLFGFPY
                     LSSVVLYCLMKRKKATVKARISEQYRPKQKKISR"
     gene            21515..>23954
                     /locus_tag="I9760_001015"
     CDS             21515..>23954
                     /locus_tag="I9760_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HAT5042792.1"
                     /translation="MLIYQGKEIVRFKEKTGGKNKSDVDGFYKDNDGGKFFIKKPGDP
                     RELFTELFAGLLLKEFMKRGLIDESYFPSLICADVIQFEDKSYGLIQPLVSFDELHKV
                     IGTSSGDGKDRNTLKETLFGPGYYAGITKQNKYFGLSMALMFSLLLGAHSVHSGNIVV
                     LNGEEKEKSKQFGRIDWGDAFRYFAHPNNNDNLLYAYENRGWFNYKSLTKDYFLNYKK
                     INGLFPAMAEKARQLQSKLNPELLVKIVTSALKNIPADLIDEKTKIQLAAYMCMDSFK
                     EATFGTEGNCKDFAIAMATLLENRLGKIAVLKDMSPLSNPEELYQSILELKPLTLLMT
                     SSTSFSETINQWADILKTTDMEKFSFDSNPINLLELVKQFNLYVDELAITCEANNVWA
                     KERIDSTPNLFALYDNSGGEAIHGHAFVPYYKESIVLRRLFTVDPNTFNLSRFAAFEG
                     PCQLYCKEHKDSAWVKIQTLLTLGNGIINTLKIIKQAQAFGIDEAVTENLKALKEQFI
                     AFQLAEADIKESLKAPSFAEPLPNKESEFFYPIDEKALAKMNGYQLATICLEELNSPK
                     PSPLIERILSNKKFWKRINSAFESGVFKGRTDDPAGKIAKIREWHQLLQISGKKTAGQ
                     IDELQKIVISLQSKIKRQTIEFEELEATLIQIKEKYQLLEKMAEQSEHEKSSAQSIIR
                     SLNLELSQLKLQLQEQEKLQFQLKELKEKIHEQTTLSKRLGEELQTQKKTNTHQEETI
                     QRITKEKSLADSSLESLRKELHELAKKERSLHKTLEEKQLQVQQLEEQLAEKEKENLA
                     LKKADKQSQHEKSLD"
BASE COUNT         7655 a         5182 c         4280 g         6837 t
ORIGIN      
        1 cttctttggt taatccgata gataaaaacc actctcccaa agaattggga ttagcatttt
       61 cttttggccc ataacaagca tagcatgctc tgcctaaagt ggggcaaagc gacccacagc
      121 ctgtttgagt tactggcccc atacaaggtt gttttttggt caccagcaca cacacattcc
      181 cttttcgttt gcactccata cattcagaat ctctttttat tttgggagtc gcattagata
      241 ataaaaaacg aatcgcctcc aatatctgcc cgctatttac aggacacccc cataactccc
      301 aatccacttt aacgtgatgc gcaatagccg tggatgtagt taaagtcttg atataatccg
      361 gtgacgcata aacactggcc atccattccc tggagtctgc aaaattacgt aaggcttgta
      421 taccgcccgc tgtagcgcaa gcacctatgg taataaggta ttgactgttt tcacgaattt
      481 tacgtattcg atgctcttca tcaggagttg aaatactgcc ttccacaaat gcgatatcca
      541 ctttcatatc cacatccacc atacctgcct ctgcaaaatg cacaatttca accaagtctg
      601 ataaagttaa caacgattca cccgcattta aaaatgccaa ttgacatcca tcacaagaag
      661 taaatttata tacagcgata cgcggtttca tagcgttcct cagacacttt aaaatccaga
      721 ttctttgaat aactctttaa cttgagaata agcaaataca ggaccatctt tacacacgaa
      781 taaaccaccg tattgacaat gaccacaatg accaattcca cattccatgt ttctctccat
      841 actgaggtag atgtcgtttt caagaatccc tttttgggta aacactttaa cggctgtatt
      901 catcatcatt tcaggcccac acatcatcac cacagagtta tctggttgta taatgatgtg
      961 gtcaatcaag tcagtcacat aaccaacacc ccaaggccat ttaggaccag cttgatctgc
     1021 ggctatataa acttcagtat gatctgattt ttgccatttg gcatactgct ttctaaaaat
     1081 aaaatcctcg ctgtgtttta ctccctgcaa aatactgagc ttaccgtaat gacgtcgtct
     1141 tcctaaaata taattgataa tggaaacaga tggagcacaa cccaatcccc cggttagaac
     1201 aataatgtct ttacctatcg ttttttgtag tggccagcct actccaaaag gacctcgtat
     1261 tccaagacga tcaccaacct gcagtttttg catggctttg gttacccgcc ctaccgctcg
     1321 aatagtgtga cttagaaaag ttttctttct gggatctgaa acaatagaaa tagccacttc
     1381 accgacccca taaaggtata gcatattgaa ttgaccagga taaaattgaa attgcttatg
     1441 atgttcttca tcagtaaagc ggaagtgcag agtaaaaatt gaagacgact cctgtgtacg
     1501 ttgaataatt tcaacttctt ttggtaaata aggatcatag actacttcat tcattttcta
     1561 ttttccttgc attggactcg cctgaaattg ctgccaactc ttcggttata tcaattccca
     1621 caggacacca ggtcacacaa cgtccacatc caacacagcc gcttgtatca aattgatcaa
     1681 accagcttcc taccttgtga gttaaccatt gcctgtattg cttttttgtg tcatcacgaa
     1741 taattttccc attgagataa gtatgacccg tcgtaaaaca ggagtcccac tctctatggt
     1801 gttcactggt acaaccatcc aggcctggct tttcaacttc actatgacaa aaacaggttg
     1861 ggcataccaa agtacaatta ccacaggata aacatcgttc ggccacgtca tcccaacggg
     1921 tgtgatttaa attcgaaaac agcaaatctc gtaaatccct tttattgtcc agaggaatcc
     1981 tcttggtttg caaggcagcg gcttgtttca cattattttg ggcatcagta cattgagcac
     2041 tttttgcatc atgtaaatcc agttgattca taatcttttt gcctttatca ctacctgctt
     2101 ccacaacaaa tccatcttca atttccgtca taagaatatc aaacgaattg ttcactgcag
     2161 ggccagttcc agcagaaaca caaaaacaat tcgcagagga ataggtgcaa ttcactgcaa
     2221 caacaaataa ttgctcgcgc cttcttttgt aacgaggatc cggcttatta ttttctataa
     2281 aaactttatc ttgaatgctc atcgccgcta aatcacaagc gcgagaaccg ataatggcaa
     2341 caggtgtttc atctggctga tgtggtttaa agcacaattt tccttgttca tcacgctcaa
     2401 ccctccaaac agtttcctgt ggcttaaaca atagaggctt tattccttga gggccattgg
     2461 caaatgcaaa agctttgcgt tcgggaattt gttccaatcg atagccacct ggttcttgat
     2521 gatcacgcat accccatggt aattgctcag ccctggtcag gacatcataa acaatcgcac
     2581 catcacgcac ttgaggcccc acacaggagt atccagcatt ttgcaaagag tcaattaaat
     2641 gttgcaattt atcatgaggc aaaaaacagt tcctcttgtc gttcatcctg gtcgtccttt
     2701 cttgtcgatc gttattactt attaacagat tctgggcaat tgctctccgg ccagccaatc
     2761 cacaattcgc aacccgccca attttgtttt cagctgaaca aaatagcgat gatcttcaat
     2821 cacctcacca atgatttcag catgtaaacc caaggggtgc gctttcatag cagttaatag
     2881 cctctctgcc ttttcagaag ggcaaactgc tattaatttt ccttcattag caacataaag
     2941 aggatcaaga cctaataatt cgcacgcgct agcaacctcg gtacgaatcg gaattttact
     3001 ttcatcaata acaaacccga ctttggattg taaggccaat tcattcagtg tcgttgcaag
     3061 ccctccacgc gttggatcac gcaagcaatg aagttctgga actgctttca ccatctcgcc
     3121 gacaagccca tgcaaagagg ctgtgtcaga ctgcactttg gtctgaaatt ccaggttatt
     3181 gcggtgcgcc ataatagcta ccccatggtc ccctataaaa ccgctcacaa tgattttgtc
     3241 accgggtttt gctgcatcac ccgaaatatg aattcccgca gggacaatcc ctactccggt
     3301 tgtcgtaata aaaataccat cgccttttcc tcgttcgaca acttttgtat cgccggcaat
     3361 gatggcaaca cctgcttgcc tggctgcatc agccatggaa taaacgattt ttcgcaaatc
     3421 cttaagagga aacccctctt caagaataaa gctggctgtc aaatacaaag gttttgcccc
     3481 tgacatagcc acatcattaa cagttccatg cactgacaaa gaaccaatgt ccccgccagg
     3541 gaaaaaaagt ggggaaataa catgagcatc ggttgccatg accatacgtc ctgaaggaac
     3601 aataaagcaa gcctgatcat ttttctgtgc caaccattca ttatcaaatg cggttaaaaa
     3661 taattgttca attagctgag ccatagcacg tccaccactg ccatgggtca aattgatagt
     3721 gccttcttta aaattcagct ttggccccgt taatttttta gttatcagga tttcattcat
     3781 aaagcattaa cccgtccata agcataaact gctgcacaag ctccctccga tgaaaccata
     3841 caggatccta aaggattttc tggcaagcaa gccttggcaa ataatttgca atcagttggt
     3901 ttttttactc ctcttaatac ctcaccacaa agacattgct tatgttcctg cgctgcactc
     3961 tccggtaatt cgaaacgctg ttcagcatca aactcagaaa aagactcttt gatctttaat
     4021 gcgctggatg gaataaaacc caaaccacgc cattcaaaac gatcgcgtat ttccaggact
     4081 tcggccatga tgcgttgtga caaaagatta cccttacgat tcactgcacg agtgtattga
     4141 atttccacat cacaacgccc ctcattcaat tgttgaatta acatcagaat agaatgcaac
     4201 acatccaacg gctcaaaccc tgatatcacg ataggttttt ggtatttttt acaaacttgc
     4261 tcataaggaa aactcccaat cacgatactg acatgggctg ggccaacaaa gccgtccagg
     4321 gcaacttcat tggcctgcag caaactatcc atagcaatgg gagttaacac atggttacaa
     4381 aatacactga aattagtgat gtttaatttt ttagcttgct taatgacaaa agcagtggga
     4441 ggtgttgttg tttcaaagcc tatagcaaaa aacacaacgt ctttatcggg gttttcctga
     4501 gcgattttta atgcatcatt cgcagaatag atcatcctga catcagcgcc gcatgctttg
     4561 gcggttaaca aacttttttg tccacttccc gggactcgca gcatgtcagc atagctgcac
     4621 agaatcactt gcggcattga ggcaagcgca atagccttat caattctcgc tataggcaaa
     4681 acgcaaacag gacaaccggg gccatgaatc atttcaacat tgttgggaag caaacttgga
     4741 atcccataac gatgaatagc atgagtatgg cctccacaaa actccataaa ctgataatgt
     4801 ctttgcggct ctgctgttgc gataatttca ttgataatgg cttgtgcgta agcaccgtta
     4861 cgaaactctt ctatgtattt cacttcaagc catcctttag catatgggca aacaaactca
     4921 atgttttgtt tgcctctctt tcatccagtt ttgttaaggc gaatcctgca tgaataatga
     4981 cgtactctcc ttcagaaacc tgctccaaaa gaacagtgga aatttccttg gtgatcccac
     5041 ccaaattaac gattgccttg ttatcttcca aaacctgatc aatttgcgct ggcaacgcta
     5101 agcacataag ccacccttat tcttttattc caacaatcca tgcttgacct agcgcaattc
     5161 caccatcatt tggtggaagc aatttgggca acaggggttt aatacctgat ttaattaatg
     5221 aattaattaa gccttccgca agaacctggt ttaaaaaaca gccgccactc aatagtagca
     5281 tgtgaatccc tttttctcga caaattcctg taacccactc gctaagacct gctattaaag
     5341 taccatgaaa cagattggcc cctaactgtt gctctgtcgt gtttgctata aactgtaaag
     5401 taggccgcat gtcaaaaaca ttgccgttta tcgtccagcc tttgtttaag acttgtggat
     5461 gagtcaccat gctttccaat ctcatggccg catgaccttc aaacaaggag agcgattcaa
     5521 cccctaaaag agcgctgacc gcatcaaaca gtcgcccaca actggtagtc agcggacaat
     5581 gaatcttttt ttctaataat tgcagtaaaa atggcgattg gggctgctct ttaaaccgtt
     5641 gcgaaatctc atcccccctt cccaaagtat gcaaaattcc agcagccata cgccagggtt
     5701 ctcttgcagc aagttcgcct ccgggctgag gaatagggaa aaaatgtcca acgcgcttaa
     5761 attccgcttt ctccagtaat aataattcgc ccccccaagc ttcgccatca gaaccaaacc
     5821 catagccatc cagaaccaat cctaatacag gctcctgaat tccatgttct gctacaacag
     5881 aagccaagtg ggcatgatga tgctgcaccc cgtaagcaga tatatcatac ttttgcgcca
     5941 atctgctggt ataaaaatca ggatgccaat catgagcgat tcgttctggt ttgaccgcaa
     6001 gaaattttaa taaatgattc aatgactcat gaaaatactc aatcgtcgct ttattgttta
     6061 aactgccaat atgttgtgaa acgaaagctt cttcgccacg agtaatacag aaggtatttt
     6121 taagatgtcc tcccaaggcc acggtagggg gaatttcata aggcaatctt aaaggagcag
     6181 gcacatagcc gcgtgcccga cgaatcaata ctgaagtgtc attaatcatg cgaacaacag
     6241 agtcatctgt gcgagtaatg atttcccgat catgagagac aattttatcg gctattgaaa
     6301 ttaaaccttg ctcggccttc ttatcatcaa taatgagcgg ttcaccgcct atgttcgcac
     6361 tggtcaccac taataccatg gaatgatact catccagcca ttggcaataa tccgggttgc
     6421 caaggaacga attaaaaagc aaataatgta atggagtaga aggtagcatg ataccgaaat
     6481 gatttaagcc cggagcaatc tcatccggta aaagcgcttt cttttttctt aacaagacaa
     6541 tcggcctttc tctgctttct aataccactc tggcctgggg gcttacttcg gcaattcttt
     6601 ctgcactttt cgcattaact accatcaaag cgaatggttt ggcctcacga ctttttctta
     6661 accgcaattg tgaaatagca tcctcatttc gagcatcaca aattaattga tatcctccca
     6721 aaccctttaa agcaataatt tctccatccc gaattctttg agctaccttg tcgactgata
     6781 gcgcgagttg cgggccacat tcactacagg ctgtaggttg cgcgtgatag cgacggttat
     6841 caggatttaa atagtcggat tgacacgatt gacaaagggg aaactgactc attgatgttt
     6901 gacaacgatc atagggcaga tttctggtaa ttgtgaacct tggaccacag tgagtacaat
     6961 ttaagaaagg ataacgatag tatctgcttt gagggtcaaa taattcaccg aggcattcag
     7021 aacaaatgca aacatccgga gtaatgacag tatttatttt accctgctgg ctatcaagaa
     7081 tttcaaaagc tctttcgttc ggaacaattg gcagtgaagt gaactccagg ttatgaattg
     7141 ctgcgagagg aggtaattgc tctcttaatt tcggaataaa ttgtgataaa tagttgcctt
     7201 gaatttctat caaaacacct gatgcattat tttgtacaaa gccagttaat tccatttgtt
     7261 tggcaagctt ataaacgcac ggtctaaaac caaccccttg cactagtcct tgaatgctaa
     7321 tttgtacccg ttccaattcc atgggctacc actttttcta tgaagaagac tggacattca
     7381 acctaaaaga atgcaacaac caatcgtgcc aatccttcaa tccaacacca ttaaccgctg
     7441 acagggtaat aatgtctatg gatggattcg tgcggcgtac atattcctta cataaatcca
     7501 aatcaaaatt cacatggggt aataaatctg ctttggttat cagaagcaaa tctgctgaat
     7561 gaaacatatc agggtatttg agcggtttat tctctccttc cgtcacagaa agaactacta
     7621 ttctgaattg ctcgcccaga tgaaatgaag ccgggcaaac gagattaccc acattttcga
     7681 taaataaaag cgattgcttt tttagggaaa tatcttgtat ggcgtgactg atcatatggg
     7741 catccaaatg gcatactttt ccagtattaa tttgccaggc atcccctcca tgagatttta
     7801 gcatttgggc atcacaattt gtttgttgat cacctactat gactgcaata tccatttcct
     7861 tgccaagttc ggaaatggtt ttggaaagca atgttgtctt cccggatcca gggcttgaca
     7921 tcaaattcaa agccaaaatt ttatgcctga gaaaataatc ccggttatga gcagcaaatt
     7981 cattgttttg cgctaaaata ttctgctcga tatcaactga atgttcatga ggcacagaat
     8041 gctcatggtg agcagcatga tgcccattat ccatggtttg acctttttct actgaacatc
     8101 cgcatatccc gcacattatt ccaccaccat tgatttcact tttaattctt caccttttgt
     8161 cagtgttaag gaatgattgc cgcaaaccag gcattcatca taatactgtt tcatcggtac
     8221 aatttgttga caagaattgc atattgcttc acctgggatt tctacaatcg ataattctgc
     8281 attttgtgca atcgtccctt gagttatcac tttaaagcta aaattcaatg cgtgcttatc
     8341 gactgctact agctgcccta tttccagcac tattttcttt acctttcggc atttattgcc
     8401 agtggcttgt tgcttgataa tctcgacaat acgcttacat aaccataatt catgcatttt
     8461 tagcctgttt aaagcaattt gcagcaattg attatagcaa tacctatgct aaaaaaagca
     8521 atgccaaatc ctactaatct tgagaaaagt aatgagaacc catcagcacg ccgaatcaag
     8581 cttgctacaa aaaatccatt taatccatcc gacatcatca ttcccagcat aaaaacaaaa
     8641 cctaataacc cggaaaatag aaaacctgat aaagcactgg ctgcaagtga aaacaataca
     8701 atttgactca tcgtatcaaa agataaggca aacaacgcac caatgaaaag aatcataaga
     8761 ggatgtacat tgttatcagc aagcttcctg aataaataac tccttaagtt ggttggtatg
     8821 atggcagaag atgaataatg aaccgtattc cacaaagtta aaaacccaaa taacaataaa
     8881 caggtaactg atacaatatt tcccacttca tttaaccatt caggagtact cgctgaaata
     8941 atacccccgc caatgatcaa actcacaata ataactacca aaccatggcc tagtgaaaac
     9001 aaaaagccaa ccatgcgcga taaagaaggt ttataacgaa ctgtgcgagt tatcgcatcg
     9061 atcgttgcca gatgatccaa atcaaaacca tgacgcatcc caagcaagag agcagttgaa
     9121 atcaaaaacc aacctgaatc gttcattagt atggggccat tattaatgtt aaagactatg
     9181 gacaatatac caatactttc actgttttta taaccctcaa tggactcaac ttccaagagt
     9241 aggttaacat atgaagctat ctatcttttg tatggtacat taatactcat tatgtctaaa
     9301 actgcaaaac ataaaaaact catctcttgc gttgttcctg tttttaatga ggaagtactt
     9361 attgctgaat tcatcgccgc attggataaa actcttaaga gcatcactta tccttatgaa
     9421 attctactga tagatgatgg aagccaggat aatactttcg caatcattca aacattaaga
     9481 aaagagtatt ctcttcgttg cattcgtttc agtagaaatt tcggcaagga aaaagcgctc
     9541 agtgcaggat tggatcatgc gcgcggagat gctgtgatct tattggatag tgatttccaa
     9601 cacccgcttg aattattaag cgaatttatc gccaaatggg aagacggtta tgacatggtt
     9661 tatgccgtga ggcaaaatcg ttctgacgaa tcatggctaa aacgagtttg cgctaaaatt
     9721 ttttatcatc tcacttccaa aataaaccgt attaatatcc cggcaaatgc aggtgacttt
     9781 agattgctcg atagaaaaat tgttaatgca ctacaaaccc ttcccgaacg taatcgcttt
     9841 atgaagggtt tatacagctg ggtcggtttt aaacaaatag ccgtgccatt taatgtacac
     9901 ccccgtaaat cgggtagttc acaatggagt ttttattctt tactggattt ggcaattact
     9961 ggcataacat cgtttactgc ttttcctcta agaatgattt ccctgggagg aatggtggtg
    10021 gcagccctgg cgcttattta tgccgcctgg attgtattaa gcacgctaat ctttggcatt
    10081 gaaacccctg gctgggcaac catagtcact gcaatcagtt tttttggcgg attgcaatta
    10141 tttgctatcg gtgtagttgg cgaatacata ggccgtgttt ttgatgaagt gaaacacaga
    10201 ccccactaca ttatcgatga ggagtccagt tttgatgacc actgctctta aattatccca
    10261 tcgattaatt tattttactg gagtagggat ctgcgctgct atcgtacatc ttttgatcgt
    10321 gttcaattta gtcaattttc taaatcttca acctctgctg tctaatgcta ttgctttttt
    10381 aattgcattc aatgtcagct atttaggtca taaatatttc acttttaatc agctacaaga
    10441 taaaaaacaa ttaagtttgc ctcatttttt tctagtggca gcttctgccg gcctacttaa
    10501 tgaaacacta tattatctgc tattacgctt cacaacgtta tattatattg ctgccctggt
    10561 ttttgtttta agtatcgttg cagtttacaa ctatcttttt tcaagatttt gggcctgtcg
    10621 ttaaattgac ttggtattcc tcacaatctt gcataaattc atcactcaaa aaataattta
    10681 gttccctggc tcttgccgct gcaataatat cactattgtc accttcacca ggatggcaca
    10741 tgattaaggt atttgtcgga acattgttta accattgcct gaataaagat ctgtagttcg
    10801 ttctggcaga aaaatcgtaa atccctgcaa aataaggatt gtgaggaata tttgttttct
    10861 ttaactggct acttaaaatt ttaccgccag ttattcccag aatttttgtt tttgcttgat
    10921 accctgacat gttaaccgaa gggtacgtgg aacgaataaa aatactcttt ccctttaatc
    10981 gctgttcata catatcaata ataattcgcc taataccagg aaactgatgg atatgttgat
    11041 gaccatcaat aaaatccggc aactttccca tgacttttat gaaatgatcc aattgagtat
    11101 tcaattcatc tgagattaat cttgaattca agatacccaa atgcgatctg ataagtaatt
    11161 ctcttaaacc aaaacaaagc ttgtcaggat gagatagaaa ataaccttct gtgagattaa
    11221 aatgcaagcc agtgcgaacc ccatttttca aagccaataa gtctggggca tcgacatcaa
    11281 aagaactggc attcacaata cagcttactg cggataagcg ctgtaaacga gccattttta
    11341 aaataccctg ggtaatacca tgattaaggc caaaatcatc agcgcacaag aatatatttt
    11401 tcagatcact cattaataac ttctaattcc aaaaaaaatt gtaatatatt actgccccta
    11461 tccttatttt gtaataacca aaatcattat ctgataatct atattatagg ggtgatagaa
    11521 atttaactcc ctgctccact tattttctta aaaggatata tagaatcagc tggggataac
    11581 taggctgtct caggttgttg cgtttttgca acaaacaggc gagttgtcag taaattaata
    11641 caaagcccac taatcaccag caatccggca accagcttcc agagataaaa tggttctccc
    11701 aaaataagga cagaactcaa aatacccaca actggaacca ataaagtgaa aggtactacc
    11761 atgccaactg gataacggct tatcaaccaa ttccatactc cataaccaac ccaagttgaa
    11821 atataaacaa tataaagcac ggaaagaatg cctttccagg tcatatgttc ataggcagtt
    11881 acaaagcttt caggcccttc aaaaaccagt gaaagtataa acatgggcaa acaggcaaca
    11941 aaactactcc aaacaatcac agcaatcaga ttaacagaat gaattttttt cgtaatgagg
    12001 ttgccaatac cccaggaagc cgctgcagca agaatacata aaaaccccat taaagacaca
    12061 ttgtggtcga agtgtaaggc cactacacca atgcctataa atgcaatcaa agcccctatt
    12121 atctgcccga cttggggttg ttctcctaaa aagattattg caaaaaacat actgaaaaag
    12181 acttgcactt gcatgagtaa agaagccact cctggagtca tgcccacacg caaaccaata
    12241 aaaagcaatg caaattgcaa ggcaaacatg attaacccat aactagcaat aattttaaaa
    12301 ggtacagcag gaggcttgat aaaaaacaca gccggtacac tggctaaaag aaatctgaca
    12361 gcgcaaagaa gcagtggaga aaattcttca agactaagct tgacgaaaat aaaattaatt
    12421 ccccaaacga aaacaaccaa taaagctaac aagagatggg caaaaggcat gactgaagtc
    12481 aacttttata aaagtctctt tattttaacc cataaacata aaatatgcta agaaatctta
    12541 ataattgagc aaataaacaa caaataattt cggcatattg gcatgaatcc taaaaaaatc
    12601 atagtattat tgatattttt ggtttttagc aaatccgaat gtactttatt aatactctct
    12661 taagtttttt ctgctaaatt tatgctaaat ttaaaaaata tactgacttt atttagctca
    12721 cactggggaa ttaaatggcc aaaactatta caaaagacag tttttttcat agtcgtttac
    12781 aacaatttgt agctaattcc ctttttccag caggggccgg tgattggtat aaaaacaaag
    12841 gctacggaaa aaagggcgaa gttgatgacg aagctccttt tagagatttc gttgaaaaac
    12901 aaaaaaccga taaaaaatca cactattata aagagtttca aggcctggat ttaagtctga
    12961 agaaagtgaa aacaaagctg gtttctggtg aatcctgtca gttagaagtc atgaaatgcc
    13021 aaccagagtc aaatgaacct aaaaaacctg gcactggcaa acatattgtc tattttcccg
    13081 gggcaaatac ttactaccaa gcctgttttc gcgatatcag cacggcttgc aaagaaactg
    13141 gagcaacagt acatgctttt aatttcccag ggacaggtct cagttcaggg aaggtgagag
    13201 aggcaaatga cttaatcaat gccggtattt ctgtggtatc ttctttgatt aaacaaggcg
    13261 tgcacccgga cgacatcatt ttacagggtg attgctacgg agcaagcatt gcattagagg
    13321 taaaaaagca attagaagac caggcagaca tcaaagttcg tgcaatcatg aacaatgttt
    13381 tcaagtcatt taaagcagcc gtttgcgata tgattaccca atctccatgg ctacctaata
    13441 tattaaaaag cattgttaaa agattgttag aatttaccgg ctggcatgtt actccgggaa
    13501 aaaaatataa acatgccgat ccttaccaat gtcatattca acacttggga gatcaaacgc
    13561 tagaatcgtc aacattatca ggtaaagtca gcaaatatca tcatgaaata caaactgggc
    13621 aaaccaaatc tcaaaaaagg gctcccatta ccgatacttg tccagaagag tatccaaagg
    13681 atcgcgatga gctggatagg aaacattatg ttcgagtcaa agaaagcgct aaagaacgcc
    13741 ttgcttccaa atttggagta gataaatttg gaagggtcaa tgctcacttt gccgatttgt
    13801 gtgaactgga aatgttggat gggcaatcgg tgtatcaagg ctttgtcaat gactatattg
    13861 ccagatcgaa tgcttatatc gaaaaacacc ctcaaaaagg tattaaagaa gttcaggatg
    13921 atttacgtaa attacattac ctgcaaccag ctgattccat agagattact gaagatgaag
    13981 ctcaggattt taatacggta gtggatctta ttacggaaga acaacaacta aggcatgatc
    14041 gattcaacga taatacaata ggtaaaagca tctctatgta attgaaagta ctgactggca
    14101 ggactattca gcttttgcca gtcagttgcc tcgaacaaaa tccttgtgct tcaacgccaa
    14161 gggcactatt aaatttactg gacatattct gaaatgcaat cagtgctgtc aattcaatga
    14221 tttcctgttc agaataatac aatttcaatt gattgaagca ctcatcatca ggacgttgat
    14281 aagaataagt tactttttca gcataatcca atgccgcttt ttcttctggt gaaaataatg
    14341 aggaactcct ccatttcgcc agttgtttat accgtgcttc acttccagcc cttttaatta
    14401 aagttgctga attaatatca acacaaaacg cacaccagtt aatttgagaa accctgacag
    14461 tgatgagaga gcgtaatatt ggggaaagtg gtgatttttt tctgtctatc acgccataca
    14521 tagcagcaac tgccaaaaat aacctgggaa ttcttgccca aatcaaagca ggttcgagta
    14581 tttggccgta ttttcttttt tgattccaga agaatggttt taaataccag ggagtcttct
    14641 tgatattttt ttccaaaatt ctcatgcata attatccttt ttatgaatac tgaattaata
    14701 gcatggaata agaatgaaag aaattatttt ggcaaccagt aaccctggaa aaataaaaga
    14761 attagaacaa ttactagccc ccaccatatg cattccccaa gccgacttgg gtatatccga
    14821 tgcggaagaa acaggattaa gttttataga gaatgccatt ctcaaagccc gtcatgccag
    14881 ctcattagcc aataaaccag ctttagccga tgactcgggg cttgtagtcc cctctttaaa
    14941 tggagagccg ggtatctatt cagcacgcta tgctggaata aaggcaaatg acgaagacaa
    15001 tattcaacaa ttgttaagta aaatggctga tttatcccaa gaacaaagac aagcctattt
    15061 tttttgcgca atagcgctaa tgcgacatgc caaagatcca acaccgctca ttgccacagg
    15121 cgtatttcat ggccttatta gcgtgaaacc ttcaggaaca aatggctttg ggtatgaccc
    15181 ggtattttat ctcaacgaat atcaatgcac agccgctgaa ttacctgcta aaattaaaaa
    15241 taggattagc catcgcgcaa aagctttaaa ccaattacgc gcccttcttc cagattaata
    15301 tgaacagtga agtagatact ctttttgcac aagcctataa gcttcaatat gaaggccatt
    15361 tacctcaagc cattagctta tatgagcaga tacttgccca atcacccaaa catacagaaa
    15421 ctctgcattt cctgggatta acctatgccc aattaggtga tatggaaaat gccattttat
    15481 attttttgca agccagaaaa attaatccca atgacgttgg tatactcaat aatctcgcta
    15541 acgcttataa aaaggcaggt caattagatg aggcaattaa atactaccag caagccattg
    15601 agattaaacc agagtatgtc caggctcata ataatctggc agcaacctac gcattactca
    15661 acaattacca gaaagcgttg catcattatg tgattgcagt gaatacagaa cctgacttta
    15721 gcgcggctca ctttaattta ggtttattac ttctcaaaaa tcaacaattg tctgctgcca
    15781 aaacacaatt taataatgtg atagctctaa atcctcagca cagagaagct caattttact
    15841 taggtatttt gcatttggaa gacaatttac tctcagaggc agaacaggca tttcataaag
    15901 tgttagagca agatcatgaa catgtccaat ccctaattaa cttaggcgtg atcgctttga
    15961 aaagagaaca aaaccaatta gctgttgatt attttaccaa agccttggct ttggataatg
    16021 aagacattga tgcacgtaat aatttggcag ccacattcat gcatcatgat cgatttgaaa
    16081 atgcgctcat gcattacgat gttttactga aaaaagaacc cgacaatctg gagtatttat
    16141 acaattccgg agtcgctcaa atggcattgg gccatctcaa tgaagccacc cttttgtttg
    16201 atcagatttt aacattacaa agtgatcaca ccccatcctt gaataatctg gccgctattt
    16261 atttaaaaat ggacatgcga gaaaccgcgc gcgaatatct tgagcgcgct ctggcaatca
    16321 accccgatga tgtgatcagc aggcatatgc tgaatgccat tacaggggca acgagcgtta
    16381 atactacaga aaaatatgcc caaaatttat ttaataatta tgccttgtat tacgaccaac
    16441 acatgcaagg tgaattacac tataagattc cgcatcacat tggccgcctc atccaccaac
    16501 ttcaactttt gcaaaccagt cattctttgg acttgggttg tggtactgga ttaacaggta
    16561 ttgtgcttcg tgaaataagc aaacacttaa ccggagtaga tatagcagaa aaaatgatag
    16621 ctcgagcaaa ggaaaaaaat atctatgatc ttctggtatg ctcggaatta atcgattttt
    16681 taagaaaaga taaaaacgat tacgatttag cggtcgctgc agacgtccta ccctattttg
    16741 gtaatctgga tgatattttt aattccatca atcaacatct gaagcaagaa gggtatttta
    16801 ttttcactac cgaaatcagc acaacaactc cgtggaaact ggaaagcagc gctcgattta
    16861 gccatcaacc cgaatacata gagagcttaa tcaataaata ccaatttcat cttatcaaac
    16921 aagagaaaat accaggccga acacaaaaca aacaagtgct ggaagtcatg ttgtattgca
    16981 tacagaaatc aacctcttgt tttgtttcca actgaactta cgccccccta atcactttgt
    17041 taaaaaaaac cgttcacagg atatgtaatt agggaatacc aagtgttctg tctttcccgc
    17101 gaaggcgaat ctatcaataa aattagcata tagtttttat ttttagacgg tttcccgcct
    17161 tcgcgggaag gaccactcaa ataaatagga ttataaacag aaaagactgc attcccaatg
    17221 aacggttgta ttaaaaaata aacttttatc taggcttttt cgtgagttct gtccaaatct
    17281 ggcaaactct gaaaaggata actctatatt ggatttttca tattcttaaa cactaaaatg
    17341 gcaaatccaa ctcagaatcc aagcgctgaa tttgtttctt aaacaaatat tgaatttgct
    17401 ccagatgatc tttatctgtc gcctcaaaac gtgcgaccag acaaggagtt gtgtttgatg
    17461 ctcgtaacaa tccccatcca tttggaaatt caacacgcaa accatcaata ttcaaaatcc
    17521 taccttcagg gaaatcagcc tgctcactaa aacgctgaat aaaatcaaat ttttcttcat
    17581 cagtaatcgc tatttttatt tcaggtgtat tgacactatt agggatcaac tcaaattgtt
    17641 cactgacagt caaatgggaa aaactgataa tctctaacaa tcggcatgcg ctatataaag
    17701 catcgtcaaa tccataccag cgatctttaa aaaacagatg cccgctcatt tcgccagcca
    17761 agatggcttg ttcttttttc atcaccgctt tcacaattga atggccagtg gggcacattc
    17821 tggcaacacc gcctgctgcc tggatttctt tctccaaatg actggagcac ttcacatcaa
    17881 acacaacagt agcacctgga ttacgactta aaatctctcg tgagtaaaac atcattaaac
    17941 gatcaggcca aatcacttcg cctttatttg tcactaggcc aagtctgtca gcatccccat
    18001 caaagcccaa accaatatca gcttgatgat ttgccacagc cgcttttaaa tcaactaaat
    18061 tagcttctat tgaagggtca ggatgatgat tgggaaatcg accatccact tcacaataca
    18121 ggggaatgac ttcacagcct aattttgaaa tagccctggg aataattgga cccgccacac
    18181 catttccaca atcaacaact acttttaaag ggcgcttcaa ttgaatatcg ctcacgatac
    18241 gctgaatgta gccttctatc acatcaaaag ccgtttctgt gccacgaaca gctgactgtt
    18301 tgacttcacc cagcaaagca taaagtgtat ctatttcatc ttgaattaat gtttttccag
    18361 ccaatactaa cttgatacca ttataatcag caggattatg acttccggta accatcaatc
    18421 cagaatcaat cccttgcgta tgggtagcat aatacatgac aggtgtcgcc acagcaccaa
    18481 gatcaaggat atctatccca ctgtctagca aaccctcttt caaggccgta gccaatgcat
    18541 gactggtcag acgtccatca cgagctacaa atatttgtcg acgatttaat ttttgtaaat
    18601 aacgtgcaat agctaaacca atgttataaa aagaattctc atccagttct ttaccgataa
    18661 tccccctaat gtcataggca cgaaagacag agcggggaat ttgtttttgt tgataggtca
    18721 tttaatatct cccggaacta ccaaaaccac cttctcctct ggagctttct gtaaattcat
    18781 tgaccacctc aaaagaagcc tgtacaaccg ggataaaaac taattgagca attctatcac
    18841 caggattaac agtaaaatgt tcctggctcc tgttccaaca agagattttt aattctcctt
    18901 gataatcgga gtcaatcagc cctacaaggt ttcctaaaac aataccattt ttgtggccta
    18961 aacctgatct tggcaaaatc actgccgcca gctttggatc ggcaatgtaa atagcaatgc
    19021 cggtgggcag caatactgtt tgctgaggtg ctacttgcat aggctccgag atacagaccc
    19081 ttaaatccag accagcggac ccatcagtgg cataggcagg caatggaatg gtatctccta
    19141 tccttgaatc cagaattttt aactgaataa cttgatgcat aataattctc aatcgtttta
    19201 ttgcgcctaa ttttgcagac ttgcagcaag gattgcaata atttggcctg ccaggcgtgt
    19261 tttatgggtt aatggcagct ctatttgttt gttttttgta atcactgtta cttgatttac
    19321 atcactttcg aatcccattc cattacctac ctgattggcg ataatcatat ccagtttttt
    19381 atttttcatt ttttcttttg cataggaaat cacatcagtt gtttcagctg caaacccaac
    19441 aacaaaggaa gctttaccac ttttagctac ttcagaaaga atatctgcat ttctaacaag
    19501 ctggagagtg agttcagagt tttcattttt tttcattttt tccgaagcag gattcagcac
    19561 tctataatct gatactgcgg ctgttccaat aaatacatct ccctgttgta catgtctgtt
    19621 cacttcttcc agcatcgatt gtgcagactc cactcttatg acctcgatgt ccgaaaacgt
    19681 ttgtaaatga caaggaccac taattaatgt cacttgtgcg cctgccatag cggcggcttc
    19741 agccattgca aaacccatct tccctgaact gtaattgctt aaataacgaa ctggatcaat
    19801 cgactcgcga gtagggcctg cagtaatcac aattcgtttg ccatgcaata gttgattcac
    19861 atccagtaag cgcaaggtat tcacaatttg atccgcctcg ctgactcttc ccaatccatg
    19921 ttccccacag gcctgggagc cttcttcagg cccgacaaaa aacacacctc gttcttttaa
    19981 caattcacaa tttgccttgg ttgctggatg tgcccacatg cttctgttca tggcaggaca
    20041 aacaataact ggcgtttcag taacgagata taatgttgaa agcaaatcat cagcaagacc
    20101 ttgagccatt ttagcaagac aatttgcaga cgcaggagca atgagcaaat aatctgccca
    20161 tctggctaat tcaatatgtc ccatagcccg ttcagcttga gtgtcgaata aatcaatcct
    20221 cacttcattg cctgataatg cctgcatcaa taaagggtta atgaactgtt gtgctgaatg
    20281 agtcatgaca accctcacat cggcccccaa acgagttaac tctctaacca gatgagcaga
    20341 tttataagca gcaatgccgc cacaaacacc aagtagaatt tttttattaa taaaatcttg
    20401 catggatcgc catttttgat aagaataacc ccgatcaaag cacaaacttc gcaataaagg
    20461 aatataaaag gaatacaaaa tgatggttgc ccatacagcg cagcaacttg acttgcgcga
    20521 aaaactactt actaatggag tacatagcct ctctgatatt gaattgttag ccgttttcat
    20581 cagttcgggt aacaataaaa aatcttgttt gcaactagcc tatgagctca ccaaacactt
    20641 gggaaattta cgtaatatcc tcaatgctga tctgcaaagc tttaaatcaa tccatggatt
    20701 aggagaggtt cgttatgcac aacttcaggc tgcgaaagaa atatgtcatc gaagtgattt
    20761 tattcatctg caaaaagaaa tccggttatc taacacacaa caaacttatg cttttttaaa
    20821 aaaacgactg cgagactaca aaaatgaaac ttttgccgca ctctttctgg ataaccaaca
    20881 ccgaattatt gcttatgaag aattattctc aggaaccatc aacacggcaa cagtctatcc
    20941 cagaccgatt gtagaacgtg tattgcaatt aaatgctgct gctttaattc tggcacataa
    21001 tcatccctcg ggcttgtctg atgcaagcca gcaagattta gccattacag aacgcattcg
    21061 agatgctctg gatttggtag atgcccggtt actggatcac attgtcattg gggataatga
    21121 agtgtattcc atttttgctg agaacaaatg ggtatgtaac tagttcccgt ccctatcatt
    21181 tgagttaatg gattaaaaat gctccaacct tttattacag attttataaa aaatagttcg
    21241 tttcaattta tttttgtaat gggattagtt tctgacatca ccgtagccgg gacgataaaa
    21301 cacatagtag ttctctttgg attcccctat ttaagctctg ttgttcttta ttgtctaatg
    21361 aagcgtaaaa aagcaaccgt taaagcaaga atcagtgagc aataccggcc aaaacagaaa
    21421 aaaattagta gatgaattct taaggttttc ttaaggttta gttaatactt ggtggttata
    21481 atggcacaaa gtgttgtttt aggtcagagt cattatgtta atttatcaag gtaaagaaat
    21541 agttcgattt aaagaaaaaa cggggggtaa aaataaaagc gacgtcgatg gtttttataa
    21601 agacaatgat ggcgggaaat tttttataaa aaaaccaggt gaccctcgtg aactattcac
    21661 ggaattattt gccggtttgt tattaaaaga attcatgaag cgagggttga ttgatgaaag
    21721 ttattttcca tcattgattt gcgcggatgt cattcaattt gaagacaagt cctatgggct
    21781 catccaacca ctggtttcat ttgatgaatt acataaagtg attggaacga gctctggtga
    21841 tggcaaagat cgtaatacac taaaggaaac cctttttggg ccaggttatt atgctggtat
    21901 tacgaaacaa aataaatact ttggcctttc tatggccctt atgttttctt tattgttggg
    21961 agcccatagt gtacatagtg gcaacatcgt tgtactcaac ggagaggaaa aagaaaaaag
    22021 caaacaattt ggccgaatag attggggaga cgcattccgt tatttcgctc atcctaacaa
    22081 taatgacaat ctcctttatg cttatgaaaa cagaggttgg tttaattata aatcactaac
    22141 taaagattat ttcctcaact ataagaagat caacgggctc ttccctgcga tggctgaaaa
    22201 agctcgacaa ttgcaatcca aactcaatcc tgaattactt gttaaaatag taaccagcgc
    22261 actcaaaaat attccagctg atctgataga tgaaaaaacc aaaatccaac ttgccgcata
    22321 catgtgtatg gattcattta aagaggcgac atttggcact gagggtaatt gcaaagattt
    22381 tgccattgcc atggccacat tattagaaaa tcgtttagga aaaattgctg ttcttaaaga
    22441 tatgtcccca ttgagcaatc ctgaagagct atatcaaagt attttggaat taaaaccctt
    22501 gacgcttctt atgacctctt caacctcttt ttcagaaacc ataaatcaat gggcagacat
    22561 cttaaaaact acagacatgg aaaaattttc gtttgatagt aatccaatca acctactgga
    22621 attagtcaaa caatttaatc tttacgttga tgaactagcc atcacgtgtg aagccaataa
    22681 tgtctgggca aaagaaagaa tagacagcac ccctaatctg tttgctctct acgataatag
    22741 cggtggtgaa gcgattcacg gacatgcctt tgttccttac tacaaagaaa gcattgtatt
    22801 gcgccgccta tttacagtag accctaatac tttcaactta tctcgttttg ctgcttttga
    22861 aggaccatgc caactctatt gcaaggaaca taaagactct gcctgggtta aaatccaaac
    22921 cttactgact ttaggtaatg gaattattaa tacgctaaaa ataatcaaac aggctcaagc
    22981 attcggaata gatgaggcag ttacagagaa tttaaaagca ttgaaagaac aattcattgc
    23041 ttttcaactt gcggaagctg atataaagga atcattaaaa gcaccttctt ttgcagagcc
    23101 cttgcctaat aaggaaagtg agttttttta tcccattgat gaaaaagcat tagctaaaat
    23161 gaatggatat caacttgcaa cgatttgtct ggaagaacta aatagcccta aaccgtcgcc
    23221 tttaattgaa agaattctca gtaataaaaa attttggaaa cgtattaact ctgcttttga
    23281 aagtggtgta tttaaaggcc gcacagatga tcccgccggt aaaatagcta aaatccgtga
    23341 atggcatcaa ttattacaga tttctggtaa aaaaactgct ggacaaattg atgagctgca
    23401 aaagatagta atttctcttc aatcaaaaat taaaaggcaa acgattgaat ttgaggagtt
    23461 agaagcgact ttaatccaaa taaaagaaaa atatcagtta ctggagaaaa tggctgagca
    23521 aagcgaacat gagaaatctt ccgcccaatc cattattcgc tctctgaatt tagaactgag
    23581 tcagcttaaa ttacaactgc aagagcaaga aaaattacag ttccagttaa aagaactcaa
    23641 agagaaaata catgaacaaa ctactctatc caagcgatta ggagaagagc tgcaaacgca
    23701 aaagaaaacc aacacgcacc aggaagaaac cattcaaaga atcaccaaag aaaaatcatt
    23761 ggccgattct tcccttgagt cgcttcgaaa agaactgcat gaattggcaa aaaaagaacg
    23821 ctctcttcat aaaacgctcg aagaaaaaca attacaagta caacagttag aagagcaatt
    23881 agcagagaaa gaaaaggaaa atctagccct gaaaaaagct gacaaacaaa gtcaacatga
    23941 aaaatccctg gata
//
LOCUS       DACTJE010000033        23595 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.033, whole genome shotgun sequence.
ACCESSION   DACTJE010000033 DACTJE010000000
VERSION     DACTJE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 23595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23595
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.033"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..2136
                     /gene="pbpC"
                     /locus_tag="I9760_001016"
     CDS             <1..2136
                     /gene="pbpC"
                     /locus_tag="I9760_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HAT5042793.1"
                     /translation="KYRLYVPLSQIPKELMEATLLQEDQYFLWHKGVNPFSMVKAIWQ
                     TYVVKSRRIGASTITMQVARIRFGINSKKISGKLWQVIRALQIERHYDKDKILEAYFN
                     LAPYGGNIEGVGAASLIYFNKPVSKLGLPEALTLSIIPQNPVKRTSKNSELKKIRNRL
                     FARWLKQHPQDQEKQPLFGLPLVMQTNQNLPFLAPHFVNMVLKENPGLSQSISTTLDY
                     RLQLIVERITRHYLARKKMFGVHNAAVLLVDSRTMSIKALMGSADFFNNKISGQINGV
                     ETKRSPGSTLKPFIYGLALDQGLIHPNTVLKDVPQSFNGYNPENFDYDFMGLIKAKDA
                     LVLSRNIPAIELSNQLTNPTLHKLLEQAHITQLRSESYYGLSLNLGGVELTMKELVGL
                     YAMLINDGVWYPINSLKGVPRQKGRHLLSAEASFLVFDMLKNTPMKESNNDGSLQLPI
                     AWKTGTSSGYRDAWTVGVFGPYVLAVWIGNFDNQANPAFIGKEIAAPLFFELINAIKH
                     ERGPIARVEKYPEHMHLEKIEVCKASGMLPGRYCTDTEWTWFIPGKSPIKTDTIFREV
                     AINGKTGLRTCHIDENTRFEVYEFWPSDLLRIFRRAGIQRHTPPFFEPGCALSGNSGI
                     SPQITSPQAGVSYIVHANTQSNKIIPLTAVTDAGIAHLYWFINESFVAETKSDEPYLW
                     QAKPGKYVVRVVDDRGLSDARDITIQMVS"
     gene            complement(2195..2695)
                     /locus_tag="I9760_001017"
     CDS             complement(2195..2695)
                     /locus_tag="I9760_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042794.1"
                     /translation="MFESFRKCLSAISMRAPYASTPGHRNAQRANAGSEVLGTTLETF
                     DPAASIPTKLAQTLISIYSLFRFDTHVSEKLIHLLQGSIAATQMGLGIALLFTGTECE
                     EYTDADLCKAIFLLQLLYRGTLLVGWAPSEFSKDPYAEPKVVSEDKAESEEENEDEES
                     RNSASV"
     gene            complement(2859..3455)
                     /locus_tag="I9760_001018"
     CDS             complement(2859..3455)
                     /locus_tag="I9760_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042795.1"
                     /translation="MFSKKERTLLFANNLWYLSEGMLGPLFGIFCERFNSSPLDISWV
                     WSLYLISVGIVVIGLGYISDFIHKEKLLVAGYALQTIFTFCYIFITSKKQLALLQIGL
                     GISGGLSTTTWYALYARYEDKTHEGLTWGLANGLSSIYTGIALLCGGLIIAKTSFDVL
                     FIIMGCIQFLATIYVSKLIFIEHPKIIRRFRNPNLAKN"
     gene            3616..4734
                     /locus_tag="I9760_001019"
     CDS             3616..4734
                     /locus_tag="I9760_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1661 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042796.1"
                     /translation="MNTVPLKPQRLLSLDVFRGMTIVLMIFVNGQAAIDPYPIFEHVD
                     WNGCTLADLVFPFFLFIVGLTSVISLKNQMERKAKTSLYSAIIERSVVLFLLGLFLNV
                     FPHPIEFDSIRIYGILQRIAVCYLISAFIYLNTSIKTQFFIFLVLLLGYWIIMTQVPV
                     PGYGANQLTKDGSWVSYFDQLFFSASHLYEKTYDPEGFLSTFTSIATTLSGVLAGSLL
                     ISPCNQFKKFYLLAGIGLLFLLLGWLWNMSFPINKNLWTSSYVLWTSGLALLAFAFCY
                     LLIDRFGVKKWSVFFKVFGMNALFAFVFHVLLLKLQYAFKITTPDGSKMVLISYLKDY
                     FFGGFSNHNAALLYSICFLFLNFLVVLILYKRNVFIRI"
     gene            complement(4828..6309)
                     /gene="legL3"
                     /locus_tag="I9760_001020"
     CDS             complement(4828..6309)
                     /gene="legL3"
                     /locus_tag="I9760_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL3"
                     /protein_id="HAT5042797.1"
                     /translation="MLTLSLAKCINATKNGAFAIKDLDLSGPLSYEDVLFLAEYLKVT
                     PLTDLDLSMAITRENSKALWDLSQVIAKNSQLQKLTFEVQLDFDFYFKHNLPIGWLDF
                     IDDYRLKKIAAPLHEALVNMLKNKPALNKLTIDFLVMETPFHNKHLNQLARSINKIPL
                     NYLNLGLSVTKETQVPLFKTKEPNQTLEFLILRGFNSGRNTLKYLPQTAALKMLAIDD
                     MQFEEHDLKLLANIMKSNSSLVALILGKTNLGQVNSPDVLTWLSACPNLNSLILIDNN
                     LSRLNVDSLCELLANNLQALKKLDLSKNAYMGDDAIKIAKALTFNTHIETLVLDDNYI
                     ENEGLQAFIDLVKNNKNITSLSISNTCRYTPSNEIIHSLCDLLRDPGCQLKELKFNQD
                     ITLAQYLMLQHALNSNKTLEYIDFNFRNQNILEAILGKNNSGNLNENEFSSDNPKEEK
                     APNTLNTDPTSKSAPLFFISPKSRQEENKNELSSLPSHSVHLH"
     gene            complement(6447..7484)
                     /locus_tag="I9760_001021"
     CDS             complement(6447..7484)
                     /locus_tag="I9760_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HAT5042798.1"
                     /translation="MKLTRNMIAQAVQAKIITEEQAKNLIEFIKNLPEQTPGFNLTNV
                     LYYFGGLIAIGAMTLFMNLGWELFGGWGIFYLSICYALLGLGLCSQFNGKGYVIPAGI
                     CATFVICLTPLAIYGLQKGMGWWPDDSVYHDYHVYIKWHWIFMELGTLMVGTILAWIY
                     RYPFMIMPIAVTLWYMSMDIAAMLGDGQFDFELRAMVSMYFGLITILIAFWVDVRSSQ
                     SADYAFWLYIFGVMAFWFGLSCQSSDSEISKFIYMCINLFMILTGVILNRKVFVIFGA
                     LGCSYYLGHLAFQVFKFSMLFPVALTAIGFGIIYLGIIWQKNEAKLTKELRSILPKPL
                     RDLLEARDSMD"
     gene            complement(7620..8936)
                     /locus_tag="I9760_001022"
     CDS             complement(7620..8936)
                     /locus_tag="I9760_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAT5042799.1"
                     /translation="MKSRLSVLSLALITVCSVDSIRNLPAAALAGNQLFYYFALALLF
                     FLLPCAIVAVWFSRQSEQGVYGWVKQGLGNQWAFIAIWFQCIQNVLIYPTLLSFIAGT
                     LLYSISPDLVQNKSMLFLIIIFLIWSLTWINLKGIHLSSRFNSYCSITGLILPFIIIL
                     FVGLYWWITQVKPGTTVLPPAEPYSWTSLTAIILSFCGIELAAVHARDSKQGAFPKAI
                     AISVITIFLTMLFGSIVLAMIIPPEQLNFISSIPQLIQLFFNKIGYGYFAFIINGLIA
                     IGCIGAANNWLIAPIKGLEFAINEGFLNRQLSQVNKHNAPFRLLVAQAFCVSIISALF
                     LIVPSINASYWIMLNAATQINLLMYLLLFISAIKIVSSKPILSKLNIMIPAYLGLAGS
                     GITLIVSLTPPPSLAISSKLIYALLGAIFLVIMTLIPIWRRPRVLT"
     gene            complement(8936..9703)
                     /locus_tag="I9760_001023"
     CDS             complement(8936..9703)
                     /locus_tag="I9760_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HAT5042800.1"
                     /translation="MFKNAIVRTPSSTMINGLTTSADLGKPNYELALIQHQNYIKALS
                     TCGLEVTVLPDLEQFPDACFVEDVSLLTEQFAVLTCPGAESRRDEVQEIEPSIQAFFK
                     DRIFRISTPGRLEAGDVLRIDNHFFIGLSERTNKEGAEQLIYLLNQHGYTASVIQLKK
                     FLHLKTGVSYLNNDYVLVSGELINHQAFGHLKQIVVSHEEAYAANCIMINETVLLPKG
                     CPKITHRLSELGFSIIELDMSEFRKLDGGLSCLSLRF"
     gene            9885..10808
                     /locus_tag="I9760_001024"
     CDS             9885..10808
                     /locus_tag="I9760_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042801.1"
                     /translation="MTSNKCDIVLLTQKDFIDPADITPYIQNVLTEDRLLTEALEQKG
                     LKVKRTHWDNRQYNWSETRYALFRATWDYFHRYDEFHLWLNDCSKRIQFINPISIIQW
                     NIDKHYLSDMQQKGINIPPTLFLKRGRLNILNNLMKESGWKKAILKPAIAGGARHTYL
                     FDQDSADHYQDVFESLINNESMLLQEFQENIVNKGEVSFMVFGGRYSHSILKKAKSGD
                     FRVQDDFGGTVHPYEASQEEREFVENVIAQCDELPVYARVDVMWDNNNKLCLSELEMI
                     EPELWFRESSQAASAMADAVCEHISGVSEYK"
     gene            10850..12523
                     /locus_tag="I9760_001025"
     CDS             10850..12523
                     /locus_tag="I9760_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family peptidase"
                     /protein_id="HAT5042802.1"
                     /translation="MNTRIVSLLAVSMASVNLQAASENIIWQAGDNNLKKYNFSILQD
                     KKGFMSITNKITSLNYQLKPVDHEGNRDYNDIRYQVYYRNIPVWGHELILHKTSKSKI
                     FLTGVDVSEIEKDVHQTDGKLSAADIESQIIKYNNNSIVYKNIKKIIYLDSHKKAYLA
                     YHLSMYANDPLHFVSAPNYIIDANSGEILKSWDDLTHKKVGQGLGGNVFILPYRSGLF
                     QHGDSMKDIPSLGKFEVTIKGKNCVVESPEVRVINAAYTDLDKRSFPVLSIVEIYKNI
                     PAFSYPCSKESQYININDGDTSPANYSFSSINDAMYFAGVTLEMYKEYYGVEKPLGDD
                     LPLRAYTHIKNFDNAFAVPSIKIKGLYLMHQQIVIGDGDTQLTAPAQSTLSHELSHNF
                     TRLHSNLVYNGQSGGINEAFSDMASIAMQDYLRKDFPWYWDGEDWSIGREATISGEPI
                     RYMDYPTKDGHSIEHFDDYHDDLDVHLTSGIFNRAFYLLAHQPNWSVRKAFQVMVDAN
                     IKYWTSGTSFEAASCGVIQAAIDRQYNKQDVINAFKEVGVICPVIGLIN"
     gene            12719..13837
                     /locus_tag="I9760_001026"
     CDS             12719..13837
                     /locus_tag="I9760_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1654 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042803.1"
                     /translation="MMDEVKRIHARQKVASAKELEKRYCIKEAMTEYLRAIDLFLQIS
                     NKTPEDINAIVNVHYDLANLYFNRRDYLSAGRHYEAAINHLTETPLKDDSYRLLTELY
                     IDLSDACYELMNQSAGDEAMANALKAFGLIQNKTFEEQQIGDPVANFKQFHIFYEKKL
                     STKSYLNSAKFMNHEYLLGEGQVARQKEQALFEQFENISISEIQQIDRSLENMLSQLS
                     LAAEPPTPNSVSGVDRVIFNPIFVNVTPSDSSYRGMAMQVLNLAKSYIQNQKISDAIA
                     TYQQAIKILNTIKSPIESDLQIVQELTQHIRYLQNKPSSTKDQFSSCYKEAQTPVIAT
                     ASEVGFFNYRPQEAFQVSDSFHSPNEMDVEYCDVDLRP"
     gene            14252..15667
                     /locus_tag="I9760_001027"
     CDS             14252..15667
                     /locus_tag="I9760_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAT5042804.1"
                     /translation="MNKGMNAFVFLIAVIAGFGGFLFGFDSSVIADVKDQVISQLALT
                     DWQWAQVVSSSLLGCILGIPISGLVADKLSRRSLLKVVALGFILGSSLCALAPGFVSI
                     LLGRFIIGICIGIASYIAPLFIAEIAPPHQRGTLVLINGLTITFGQAIAYLIGYFLHD
                     YSLMSWRYLFWIGSLPALVLFSGMYFVPHSPRWIMIKYGADATLKTLKQIRPVGYNIQ
                     QELTEISNNLVNPAPAYIQMFKKPIVFVLLLGVGLGLFQQFSGINALMYYGPVVFESF
                     GFSPVKNAILATFFLGLVNFIFTVVTLFYVDKLGRRFLLISGTLIASISLYFVSLLSC
                     HSLFVNKFWILGGLSFYVMGYCISLGSLFWVLISEIYPLSVRGLAMSIATMFQWGANF
                     VVSLLFLPIYQFSGQTVTFAMFATFCLLACWFIYYFVPETTSVSLEKLEENLSSGKKV
                     RDIGQPVSKNIKIAKFNLIMD"
     gene            15670..16674
                     /gene="iolG"
                     /locus_tag="I9760_001028"
     CDS             15670..16674
                     /gene="iolG"
                     /locus_tag="I9760_001028"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAT5042805.1"
                     /translation="MKKKICRIGIVGAGRIGRLHAENILFHLPQFELVAIADPQIDRN
                     WAEKLGIAIASTQSEDVLCHPDLDAILIASPSHLHLEQIITASIHEKAIFCEKPIGLN
                     EEEIEKTLNIIKNNNTLLQLGFNRRFDPGFANIQKRVQFGEIGTPHILRITSRDPACP
                     SKEYCATSGGIFMDMSIHDFDMARFLMQSEVVEVYATGAVLINPDFEQFDDVDTAIIQ
                     LRFANGAFGVIDNSRQAIYGYDQRIEVFGSGGMLLAENQFEHTVSRYSANGTDGAKPQ
                     YFFLERYHQAYLAELNAFYSAWNDNQTSPVSGLDGLQALRIAKAAKQSLETHLPVVLS
                     "
     gene            16682..18568
                     /gene="iolC"
                     /locus_tag="I9760_001029"
     CDS             16682..18568
                     /gene="iolC"
                     /locus_tag="I9760_001029"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAT5042806.1"
                     /translation="MKQFPGFYFDKNRAIDLICMGRVAVDLYAEQIGLDLKDVASFKK
                     YLGGCAGNIAVGAARLGLKSQMFSCVGSDELGKFLKEELEREGVNIELLLETDNHLTG
                     LVLLGIKPPSNFPLLFYRNDCADMQIKSDQISFTDLKNAKSLLITGTGLSTESMKNTT
                     LEVVHLARKAETTIVFDLDYRPVLWRLTVIGNGESRYCQNEYVTQVYQQILPLCDLIV
                     GTEEEICIAGGSNDIEVSLINIRQRTDAPIVVKLGEKGARVHFGADKGLLQAKSFPVE
                     VLNVLGAGDGFMSGLLAGLLQGKSWEQAVTYANACGALVVTRHGCAPAIPYKEELNYF
                     IQEYDHEPEIWNSSQLTQLHEKLSKNRKTQLLIKNPCGFADGLNSIVTMQDSPTAMSF
                     SSLKLNKGQSHQFNSSYEFAALLMTGKVVFQYGCYSQEVERTDYFSQSPYVLHCSKNE
                     VSSVIALTDCEILLMETENENLFSPILFDASNMLELDNRGEGVLENTSYRLVRTVFDK
                     RNRPESNLVVGEIITFQGRWSSYPSHYHQQPEIYHYRFSEPQGYAFGENGKEVMRIEH
                     YDTYQIANNQEHAHCTAPGYALYTLWFIRHLNNNAYTLPTFTKDHEWTRTSRANLRTW
                     QHKQ"
     gene            18607..20478
                     /gene="iolD"
                     /locus_tag="I9760_001030"
     CDS             18607..20478
                     /gene="iolD"
                     /locus_tag="I9760_001030"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAT5042807.1"
                     /translation="MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTG
                     LGEALEYDAQGLIYYQGHNEQGMAHAATAYAKQKNRLGIMACTSSIGPGATNMITAAA
                     TATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ
                     LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAML
                     TEAIRHIRTAKQPLIIAGGGVHYSLATETLSEFALKHRIPVAETQAGKSSLTAKHPMN
                     VGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQDCQIIHLNVSRLDAM
                     KMNGVMLKGDAKAGLEQLGKELADYQTSLSYQQLIRHYQNEWAQELDKLTQSYSTEQA
                     GLHQTTVLGLLNQYVTEEDVIISAAGSLPGDLHRIWQSKKAKDYHLEYAYSCMGYEVA
                     AGLGVRIAKENQEGEVYVLVGDGSFVMMHSELLTAIQERKKITILIFDNHGFQCIRNL
                     QEGNGSLGFGNEFRYREPTTNALNGDYLPIDFCKYAEGLGAATYFVKSYEQFRTTLDS
                     AKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYQMMREQIETVRQY"
     gene            20493..21377
                     /gene="iolE"
                     /locus_tag="I9760_001031"
     CDS             20493..21377
                     /gene="iolE"
                     /locus_tag="I9760_001031"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAT5042808.1"
                     /translation="MSIQLGIAPINWCNDDDPNLGKDISFEQCINEMAMAGYSGTELG
                     NKYPRDVRTLKAALEQRGLQLSSSWFSTYFTEANAFEKTVSRFLEHLSFMRALGASYI
                     NVCECGHAIQGTDLPILSAHIPQFTQEQWSLLIQGLHTLGRIAYDFNMKIVYHYHAGT
                     GVFWEKDIDFLMTNTSPKLVSLLLDTGHAAFSGIDPVNLIDKYGDRILYVHLKDIRPE
                     VLRRVEMERLSFMDAVRRGVFTVPGDGAIDFIPIWAGLQKHRYNGWMIVEAEQDPEKA
                     HPLEYAKKAYDYLHSFIH"
     gene            21499..21756
                     /locus_tag="I9760_001032"
     CDS             21499..21756
                     /locus_tag="I9760_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HAT5042809.1"
                     /translation="MSMKDKLVQSAISAFLVLIASNTAIAEDLSKEKCYGVVKKGMND
                     CSTATSDCAGSSTKDSQKDAFILLPKGICEKLVGGSLDMGK"
     gene            21784..22377
                     /locus_tag="I9760_001033"
     CDS             21784..22377
                     /locus_tag="I9760_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAT5042810.1"
                     /translation="MKRIYRHFIAPILAVFVLSSYVYAEPQTLTLDNQHTYVLWKIKH
                     LGFSTQAGKWYASGQLVLDKDNPQQSKVNVTIKVDDIVTGIPELDKHLKGKLFFDTKQ
                     FPTATFVSNRVEVMGKNKAKVYGMLTLHGVTKPIILNVILNKAGINLLNDRETAGFSA
                     TTSLKRSDYGIKALIPEVGDDVEIEIEAEAYLDKKYG"
     gene            22381..22911
                     /locus_tag="I9760_001034"
     CDS             22381..22911
                     /locus_tag="I9760_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAT5042811.1"
                     /translation="MQLKNTSTSFGLVAIFFHWIMAALIIGLLGVGLYMVRIPISLEK
                     LKLYGWHKEYGFLVLFLAFFRLIWRLTNQLPELAIPLLEKIAARSMHWAFYFFMFAMP
                     ISGWLITSAAGLPASFFGLFVLPDLVAPDENRRILFQLIHEWLGYALIMAIILHAAAA
                     LKHHFINKDDILRRML"
     gene            22911..23486
                     /locus_tag="I9760_001035"
     CDS             22911..23486
                     /locus_tag="I9760_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAT5042812.1"
                     /translation="MNVYIKSSLWVLIFFVNTLIYASKLPEWEIVPAQSEITFTGTQN
                     GAPVTGRFKKFTGQIFADPSNYKTGSIDITIDMNSIAAPFEDIVTTLASPDWFNVKTF
                     PNAEFKASKFNQLDDKTYEAEGILTIRDKSAPVTLKFVVEQISNDQALVEGSSTIKRS
                     TFGVGQGEWASTDEIQDAVIVRFKISATRKK"
BASE COUNT         7414 a         4004 c         4786 g         7391 t
ORIGIN      
        1 aaatacagac tttatgtacc gttatctcaa atacccaagg agttaatgga ggccacactt
       61 ttacaagaag atcagtattt tctctggcat aaaggagtga accctttttc catggtgaaa
      121 gccatctggc aaacttatgt ggttaaatcg cgcagaatcg gggcttcaac aatcacaatg
      181 caggtggcac gaattcgttt tggaattaac tccaagaaaa tttctggaaa attatggcaa
      241 gttattaggg cgttacaaat agagcgtcat tatgataaag ataaaattct tgaggcttat
      301 ttcaatttgg cgccttatgg tggaaatata gagggagtag gggcggcgag cttaatttat
      361 tttaataagc ctgttagtaa acttggttta ccagaggcac taactttaag cattatccct
      421 caaaatccag taaaaaggac atcaaaaaac agtgaattaa aaaaaataag aaatcgctta
      481 tttgccagat ggctgaagca gcatccgcaa gaccaggaga agcagcctct gtttggactg
      541 cctttagtta tgcaaaccaa tcaaaaccta cccttcctgg ctcctcattt tgtaaatatg
      601 gtactgaagg aaaatcctgg tttatcacaa agtatttcaa caactttgga ttacaggctt
      661 cagttaatag ttgagcgaat tacaaggcat tatttagcaa gaaaaaaaat gtttggtgtt
      721 cacaacgcgg cggttttact tgtggatagc cgtactatga gtattaaagc gttgatggga
      781 tctgctgatt tttttaataa caagataagt ggacaaatca atggtgtaga aaccaaaagg
      841 tcgcctggct caactctgaa accatttatt tatggattag cactcgatca aggtttaatt
      901 caccccaata cagttttaaa ggatgtacca cagagtttta atggttataa tccggaaaac
      961 ttcgattatg attttatggg gctcatcaaa gcaaaagatg ctttggtact tagtcgtaat
     1021 atcccggcca tcgagctttc caatcaatta actaatccaa cattacataa attacttgaa
     1081 caggcgcata taactcagtt gcgttctgaa tcatattatg gcttgtctct taatttgggt
     1141 ggtgttgaac tcactatgaa agagttagtc ggtttatatg ctatgctgat taatgatggg
     1201 gtctggtatc caatcaattc attaaaaggg gttccgcgac aaaaaggtcg acacttgctt
     1261 agcgcagaag caagcttttt agttttcgat atgctaaaaa atactccaat gaaagagtcg
     1321 aataatgatg gcagtttaca attaccaatt gcctggaaaa caggaacttc atcaggatac
     1381 agagatgcct ggacagttgg agtttttggt ccttatgtat tggcagtttg gataggaaat
     1441 tttgataatc aggcaaatcc ggcttttata ggtaaagaaa ttgctgcgcc tttatttttt
     1501 gaattgatta atgcaataaa acatgaaaga ggaccaatag ctcgagttga aaaataccct
     1561 gaacacatgc atttggaaaa aatagaagta tgcaaagcct caggtatgct tcctggtcga
     1621 tattgtacgg atacagaatg gacctggttt attccaggta aatcccctat taaaacggat
     1681 acaatttttc gagaggtagc aattaacggg aagacggggc ttcgtacttg tcacatcgat
     1741 gaaaatactc gttttgaagt gtatgagttt tggccctccg atttattaag aatattcagg
     1801 agggcaggga ttcaacgcca taccccgcca ttttttgaac caggttgcgc tttgtcggga
     1861 aatagtggta ttagccccca aattacatct ccccaggcag gggtaagtta tattgtccat
     1921 gctaataccc aatcaaacaa gataatccca ttaactgcgg taacagatgc tggaattgca
     1981 catttatact ggtttatcaa tgaaagtttt gtagctgaaa caaaatcgga tgaaccttat
     2041 ctctggcagg cgaaacctgg taaatatgtg gtaagggtgg tagatgaccg tggtttatct
     2101 gatgcgcggg atattactat tcagatggtt agttgacgca tatttgataa tatataagtg
     2161 agcccttgtc ctaatcggag caagggattt aaaattaaac tgaggccgag tttcttgact
     2221 cttcatcttc gttctcttct tcagattccg ctttgtcttc tgatacaact ttaggctcag
     2281 catagggatc cttggaaaat tcactagggg cccagcccac tagcaaggtt cctctgtata
     2341 gtaattgtaa tagaaatatt gccttgcata gatcagcatc cgtatattcc tcacactctg
     2401 ttccagtaaa aagcaaagca ataccaagac ccatttgtgt tgccgctata ctaccttgca
     2461 aaagatggat cagtttttca gaaacatgag tatcaaatct gaatagggaa taaattgaga
     2521 ttaaagtttg agcgagtttt gttggaattg atgcggcagg atcaaatgtt tcaagcgttg
     2581 tgcccaatac ttcggatccg gcattagcgc gttgagcgtt acgatgtccc ggtgtcgagg
     2641 catacggagc tcgcatagaa atggcagaca aacattttcg aaaagactca aacataatca
     2701 tatccttata acaatttaat acgtgatatc tttaataaaa gcggacatta acagacaagt
     2761 tattttgatt ccagtatttc ttgatgaatt tagcatcaat ctacatcaaa aaatgttcat
     2821 ttgatttgac atttcaacat ggctttcaga ttttaaaatt aatttttagc caaattagga
     2881 tttctaaaac gcctgataat tttggggtgt tcgataaaga tgagcttact gacataaata
     2941 gttgctagaa attgaataca tcccatgatg ataaacaaaa catcaaatga tgttttggca
     3001 ataattagac caccacataa caaggcgatc ccagtgtaaa tacttgataa cccattggcc
     3061 aaaccccaag tgagtccttc atgcgtttta tcttcatagc gggcatacag agcataccag
     3121 gttgtagtag agagcccacc tgatattcca aggccaattt gcaataaagc cagttgcttt
     3181 ttacttgtta taaagatgta acaaaaggta aatatggttt ggagtgcata ccccgcaacc
     3241 agcaattttt ctttatgaat aaaatctgaa atataaccca agccaattac cacaattcca
     3301 actgaaatta aatacaaaga ccatacccat gatatgtcta agggagacga gttgaacctt
     3361 tcgcaaaaaa taccgaagag cgggcctaac attccttctg acaagtacca caaattattg
     3421 gcaaataata atgtacgttc ttttttacta aacattaatt caacttcaag ttaagtcaat
     3481 catgactaac atacaagttt aaaagagatt ttcatataga ttttagaaat ttaattttta
     3541 tttttgatat tacaatttgc cactcgtggt aagataatgg ggctcagttt tttataaatc
     3601 cattttaaat agcgcatgaa tactgttcct ttaaaacccc aacgcctttt atcccttgat
     3661 gtatttcgcg ggatgactat tgtattgatg attttcgtta atggtcaggc agctattgat
     3721 ccctatccga tttttgagca tgtggactgg aatggttgta ctttggcgga tttggttttc
     3781 ccatttttct tgtttatcgt tggcttaacg agtgttataa gcctgaaaaa ccaaatggaa
     3841 cgaaaagcaa aaacctcatt atattctgcc atcatagaga gaagtgttgt tttgtttctt
     3901 ttaggattat ttttgaatgt ttttccccat cctattgaat ttgatagcat aaggatatat
     3961 gggattctgc agcgaatcgc tgtttgttat ttaatttccg ctttcattta tctgaatact
     4021 tcaattaaaa ctcaattttt tatttttctt gttcttttat taggatattg gattattatg
     4081 actcaagtac ctgtcccagg atatggagca aatcaattaa ccaaagacgg aagttgggtt
     4141 tcatactttg accagttatt tttttcagct tcccacttat atgaaaaaac gtatgatccc
     4201 gaaggttttt taagtacttt tacatctatt gccactactt tatcaggtgt cctggccggt
     4261 agtttactaa ttagtccatg caatcaattc aagaaatttt atctattagc agggattggc
     4321 ttgctatttt tgttattggg atggttatgg aatatgagtt ttcctattaa taaaaattta
     4381 tggactagct cttacgtact ctggaccagc ggcctggctc tgcttgcctt tgccttttgc
     4441 tatttgctga ttgatagatt tggggttaaa aaatggtccg ttttttttaa agtatttggc
     4501 atgaatgcat tatttgcttt tgtatttcat gtccttcttt taaaactgca atatgctttt
     4561 aaaataacaa cacctgatgg ctctaaaatg gtattgatat cctatctcaa agattatttt
     4621 tttggaggat tcagtaatca caatgcagca ttgctgtatt ctatctgttt tcttttcctg
     4681 aattttctgg ttgttttgat tttatataaa cgtaatgtat ttattcgaat ataaagaggt
     4741 aaggttggtt gggcctcgca gattgctcag cgccaactgt gtacatcgct agtgagaaat
     4801 tataattgag tgtaatgtgt attatcccta atggagatgg acactatgac tcggtaaaga
     4861 agaaagttca tttttattct cttcttgccg actctttggg gaaataaaaa ataatggagc
     4921 acttttgcta gtaggatccg tattcaaagt atttggggct ttttcttctt taggattgtc
     4981 acttgaaaac tcattttcat tcaaattacc ggaattattt ttcccaagaa ttgcctcaag
     5041 aatattctga tttctaaaat tgaaatcaat gtattctagt gttttgttag agtttagggc
     5101 atgttgcagc ataaggtact gtgccaaagt aatatcctgg ttaaatttta gttcttttaa
     5161 ttgacatcca ggatcacgta ataaatcaca tagactgtga attatttcgt tagaaggtgt
     5221 atatcgacag gtgtttgaaa tagatagaga cgttatattt ttattgtttt ttaccagatc
     5281 gatgaaggct tgtagcccct cattttcaat atagttatca tccagcacca atgtttcaat
     5341 atgagtattg aaagttaacg ctttggcaat ctttatcgcg tcgtcaccca tataagcatt
     5401 tttggacaga tccagttttt tcaaagcctg gagattattc gctaataatt cgcataaact
     5461 atccacatta agcctgctta gattattgtc aatgagtata agactattta aattggggca
     5521 tgcactgagc caggtgagta cgtctggcga attgacttgt ccaagattag ttttaccaag
     5581 gattaaagca accagtgacg agttggattt cataatatta gccagcaact tcaaatcatg
     5641 ttcttcgaat tgcatgtcat caatagctaa catttttaag gctgctgttt ggggtaaata
     5701 tttcaaggta tttctacctg aattaaaacc acgaaggatt aggaactcaa gagtttgatt
     5761 cggttctttc gtcttaaata gcgggacttg tgtttctttg gtcactgaaa ggcccaaatt
     5821 caaataatta agcgggattt tattgataga tcgtgctaac tggttgagat gtttattgtg
     5881 aaaaggagtt tccatcacta aaaagtctat agttagctta tttagagcag gtttgttttt
     5941 tagcatgtta accaatgcct catggagtgg cgccgctatt ttttttaacc gataatcgtc
     6001 tataaaatct aaccaaccaa ttggcaggtt atgtttaaaa tagaaatcga agtccagttg
     6061 cacttcaaat gttagttttt gtagctgtga atttttagca ataacctgac ttaaatccca
     6121 tagcgcttta gagttttccc tagtgatggc cattgacaag tcaagatctg ttaagggtgt
     6181 gacttttaga tattcagcta aaaataatac atcttcataa gatagagggc ctgataaatc
     6241 caaatctttg atagcgaatg ccccattctt tgttgcatta atacatttgg caagtgataa
     6301 agttaacata attactcctc aagtcctttt gaaagaaaat aatgatgaac tcatctatct
     6361 tctacttcag gtagttgaaa gtaaagagtt acctacaatt tatctaaaga gtaatagaat
     6421 gtgataggac gtgagaaaat atttcattaa tccatactgt cccttgcttc caataagtct
     6481 cttaatggtt tgggaagtat ggatcgaagc tctttagtaa gtttcgcttc atttttttgc
     6541 cagattattc ctaaataaat gataccaaaa cctatggcag tcaaagcaac agggaaaagc
     6601 atactgaatt taaacacctg aaatgccaga tgacccagat aataagaaca tcctaacgca
     6661 ccaaaaataa cgaatacttt tctgtttaaa atgactccag tcagaatcat gaagaggtta
     6721 atgcacatat agataaattt ggatatttca ctgtcggagg attggcagct taaaccgaac
     6781 caaaaagcca tgacaccgaa tatatacagc caaaaagcat aatctgctga ttgactggat
     6841 cgaacatcaa cccaaaaggc aatcaatata gtaattaagc caaagtacat tgaaaccatt
     6901 gcccttagct caaaatcaaa ttgaccgtca cccaacatgg ctgctatatc catggacata
     6961 taccaaagag tgaccgcaat tggcataatc ataaaaggat accgatatat ccaggccagg
     7021 atagtaccta ccattagcgt acccaactcc atgaagatcc aatgccattt gatataaaca
     7081 tgatagtcat gataaacgct gtcatctggc caccagccca ttcctttttg taagccatag
     7141 attgccaaag gagtaaggca aatgacaaaa gtagcgcaaa tgccagcagg aatgacatat
     7201 cctttcccat tgaattgact gcaaaggcct aggccgagta gggcataaca aattgacaaa
     7261 tagaaaatac cccagcctcc aaacagttcc catccaagat tcataaacaa agtcattgca
     7321 ccaatagcta taagtcctcc aaaataataa aggacattgg ttaagttaaa tcctggagtt
     7381 tgttcgggaa gattttttat gaattcaatg aggttcttgg cctgttcctc tgtaattatt
     7441 ttagcctgga cagcctgagc aatcatattc cgtgtcaatt tcatcatcat ctccgatgag
     7501 tactgaattc ctgctaaaat ttaacctgat tttacaataa ctaatgatta atatctcaat
     7561 tatagctgag agtatgaaac ttgcgtctga atttgcaaat caggattgga tatttctttt
     7621 catgtaagaa ctctaggtct cctccaaata ggaatcaagg tcattatgac caggaaaatt
     7681 gcgccaagca atgcataaat caatttggag ctaatggcga gtgatggcgg tggcgtcaaa
     7741 cttacaatta aggtaatgcc actgcctgcc aaccccaggt aagcgggaat cattatattt
     7801 agcttactta aaatcggttt tgaggaaact attttgatag cgctaatgaa gagcaagagg
     7861 tacattaata aattaatttg tgtcgctgca ttcaacatga tccagtaaga tgcattaatc
     7921 gaaggtacga taagaaataa agcactaata atcgatacac agaaagcttg tgcgactaac
     7981 aatctgaaag gtgcgttatg tttattcact tgactgagtt gcctatttaa gaacccttcg
     8041 tttattgcaa attctaatcc ttttataggt gcaatgagcc agttatttgc tgcacctata
     8101 caacctattg cgatcaatcc attaataata aaggcaaaat atccataacc aattttatta
     8161 aaaaataatt gtatgagctg gggaatgctg ctgataaaat ttaattgttc tgggggaatg
     8221 atcatagcca atacgatcga accaaataac atcgtcagaa agattgtaat aactgaaata
     8281 gctatggctt tagggaaagc cccttgttta ctgtcccgtg cgtgaaccgc ggctaattca
     8341 ataccgcaaa atgacaaaat aattgcagta agcgaagtcc aggaataagg ttcggctggt
     8401 ggtagaactg tggtaccagg tttaacctgc gtaatccacc agtacaatcc cacgaacagg
     8461 ataataataa aaggaagaat taatcccgtt atggagcaat aagagttaaa acgacttgag
     8521 agatgaattc cttttaaatt aatccaggtt aaactccaaa tcaaaaaaat aataattaga
     8581 aaaagcatac ttttgttttg aactaaatca ggggagatgc tgtagagcag ggtgccagca
     8641 ataaaactca ataaggtagg gtaaattaaa acattctgaa tacattgaaa ccaaatggcg
     8701 atgaatgccc actgattccc tagtccttgt tttacccacc catacacacc ttgttctgat
     8761 tgcctggaga accaaactgc aacaatggcg caagggagaa gaaagaatag caaggccaat
     8821 gcaaagtaat aaaacagctg gttccccgct aaagcggctg ctggaagatt tctaatgcta
     8881 tcaaccgagc aaacagtgat taaggcaaga cttaaaacag ataagcggct tttcattaaa
     8941 atctcaacga caagcagctt aaaccaccat ccaactttct aaattcgctc atatccaatt
     9001 caataattga aaaccccagt tcggataaac gatgggttat tttaggacac cctttgggta
     9061 ataacacagt ctcattaatc ataatgcaat tagctgcgta cgcttcttca tgacttacta
     9121 ctatttgttt taaatgacca aatgcttgat gattgattaa ttctccacta accaatacat
     9181 aatcattatt caggtaactc actcctgttt taagatgcag aaattttttt agctgaatta
     9241 ctgaggctgt atagccatgc tgattcagta aataaattag ttgctcagcc ccctctttgt
     9301 tagttctttc tgagagacca ataaaaaaat gattgtctat tctaagcaca tctccagcct
     9361 caagtctgcc aggcgtgctt attctgaaaa tcctgtcttt aaagaatgct tgaatgctgg
     9421 gttcaatttc ctgaacttca tctcgcctgg actctgcgcc agggcatgtc aagacagcaa
     9481 attgttcagt taataatgac acgtcttcta caaaacaagc atccgggaat tgttctagat
     9541 ctggaagcac tgtcacttca agaccacatg ttgatagtgc tttgatataa ttttgatgct
     9601 gtattaatgc aagctcataa ttgggtttcc ccaaatcagc cgatgtggtt agtccattga
     9661 tcattgtaga agatggtgtt cttactatag catttttaaa cattcatcat gtccttaaat
     9721 aagtgatttc caatacacac gcagccagag attaaacaaa gagtcttcaa aatgcaatca
     9781 ttgttaattt gattaaatta gctggttgat ttgcgatggg ggtatataga tttagtttaa
     9841 aaagataagt attcttgtat catttgatac ggattgtttc tatcatgacc tctaacaagt
     9901 gcgatattgt cctactaaca cagaaggatt ttatagaccc ggccgatatt actccctaca
     9961 ttcaaaatgt gctaacagaa gatagattat taaccgaagc tctggagcaa aagggattaa
    10021 aagttaaacg aactcattgg gataatcgac aatataattg gtcagagact agatatgcac
    10081 tgtttcgtgc cacctgggat tattttcatc gttatgacga gtttcatctc tggctaaatg
    10141 attgctcaaa aagaattcaa tttataaatc cgatttctat tattcagtgg aatattgata
    10201 aacattattt gagtgatatg cagcaaaaag gaattaatat ccctcctact ttatttttaa
    10261 aaagggggag gctaaatatc ttaaataatt taatgaagga atcgggatgg aagaaagcga
    10321 tactaaaacc tgcaattgca ggcggagcac gccatacata tttatttgat caggattccg
    10381 cggatcatta tcaagatgta tttgaatcat taattaacaa tgagtcgatg ttactccagg
    10441 aatttcagga gaacattgta aataaaggag aagtctcttt tatggtattt gggggtagat
    10501 atagccattc tatactaaaa aaagcaaaat ctggcgattt tagagtacag gatgatttcg
    10561 gtgggactgt acatccttat gaggcatctc aagaagaaag ggagtttgtt gagaatgtca
    10621 tagctcaatg cgatgagtta cctgtgtatg cacgtgttga tgtcatgtgg gataacaata
    10681 ataaattgtg tttgagtgag ttggaaatga tagagcctga gctttggttt agggaaagca
    10741 gtcaagccgc gtctgctatg gctgatgcag tatgcgaaca catatcagga gtcagtgaat
    10801 ataaataaac gctgtaagac acaacaatag tcaaagacag ggaaaaaata tgaacacacg
    10861 gatagtttca ttattagctg tttctatggc aagtgttaat ttgcaagctg cttcagaaaa
    10921 tattatttgg caggcgggcg ataacaattt aaaaaaatac aattttagta tcttgcaaga
    10981 taaaaaagga tttatgtcaa ttactaataa aatcacgtcg ttaaattatc agctgaaacc
    11041 agttgatcat gagggcaatc gtgattataa cgatattcgt taccaggttt attatagaaa
    11101 tattcctgtt tggggtcatg agctaatcct tcataagacc agcaagtcaa aaatttttct
    11161 gaccggtgtc gatgtgtctg aaattgaaaa ggatgttcat cagactgatg ggaaattaag
    11221 tgctgctgat attgaaagcc agataataaa atataataac aattcgatcg tatacaaaaa
    11281 tataaaaaaa atcatttatc tggattcaca taaaaaagct tatctagctt atcatttatc
    11341 aatgtatgca aatgatccat tacattttgt ctccgctccg aattatatta tcgatgccaa
    11401 ttctggtgag attttaaaaa gctgggatga tttaacacat aagaaagttg gtcagggctt
    11461 aggtggtaat gtattcattc ttccctatcg gtcgggattg tttcaacatg gggattcgat
    11521 gaaggatatt ccttctctcg gcaaatttga ggtaacgatc aaggggaaaa attgtgttgt
    11581 agaaagccct gaggtaaggg tcattaatgc tgcgtatact gatttggaca agagatcctt
    11641 ccccgttcta agcattgtgg aaatctataa aaatatacca gcattttcct acccctgtag
    11701 caaagaatca caatatatca atattaatga tggagataca tcacctgcaa actacagttt
    11761 ttcttcaatt aatgatgcta tgtattttgc cggagtcaca ctggaaatgt acaaagagta
    11821 ttatggcgtt gaaaaacctt taggtgacga cttgcctttg cgagcctata cccatattaa
    11881 aaattttgat aatgcctttg ctgtgccttc tattaaaata aaaggattgt acttaatgca
    11941 tcaacaaatt gtgattggtg atggcgatac tcaattaaca gcaccggcac agagcacttt
    12001 atcgcatgag ttgtctcata attttaccag attgcattct aacctggtct ataatgggca
    12061 atccggagga atcaatgaag cattctcgga tatggcatcg attgctatgc aggattactt
    12121 gcgtaaagat tttccatggt attgggatgg tgaagattgg tctatagggc gtgaggctac
    12181 tataagtggt gagccaattc gctacatgga ctatccaacg aaagatggtc attctattga
    12241 gcatttcgat gattatcatg atgacttgga tgttcatctg accagcggga tctttaacag
    12301 ggctttttat cttttagccc atcaaccgaa ctggtcagtg agaaaggcat ttcaagtgat
    12361 ggtagatgcc aatataaaat attggacatc agggacttca tttgaagcag cttcttgtgg
    12421 tgttatccaa gcggcaattg acaggcaata taataagcag gatgtcatca atgcttttaa
    12481 agaggtagga gtgatttgtc ctgttatagg tctcataaat taaatacagc aattcaggtg
    12541 acttcgatac tgattgctga taaaaagaga taatcatatt aattcaactt tccggctcac
    12601 aaccaattct tttttaattt cagacaatta agaaaattcc tatttgtttt aagggggttg
    12661 gattatttta caataagttg tctttatata tgggtctgaa ttcgcattaa aggagtcaat
    12721 gatggatgaa gtaaagagaa tacatgccag acagaaagtc gcctctgcca aagagcttga
    12781 aaagagatat tgcataaaag aagcaatgac cgagtatcta agagcaattg atttattttt
    12841 acaaatttcc aataaaactc ctgaggatat taatgcaata gttaatgtac attatgatct
    12901 ggcgaacctg tattttaata gaagagatta tttaagcgcc ggtagacatt atgaagcggc
    12961 gataaatcac ctcacagaaa ccccactcaa agatgactct taccgacttc taacagagtt
    13021 atatattgat ttatctgatg cgtgttatga gctgatgaat caatctgctg gggatgaggc
    13081 aatggctaat gcactaaaag catttggatt gattcaaaat aaaacatttg aagaacaaca
    13141 aattggggat ccagttgcta atttcaaaca atttcatatt ttttatgaaa aaaaactatc
    13201 aacaaagtct tatttaaact ccgcaaaatt tatgaatcat gaatatctgc tcggagaggg
    13261 gcaagttgca aggcagaagg agcaagcact ttttgagcag tttgaaaata tatcgattag
    13321 tgaaatacaa caaattgatc gtagcttaga aaatatgtta tcgcaattgt ctttggcagc
    13381 agaaccccca acccctaatt cagtctctgg agttgatcga gttattttta atccgatttt
    13441 tgtaaatgta acgcctagtg atagctctta tcgcggcatg gcaatgcaag ttctaaattt
    13501 agccaaatct tatatacaaa atcaaaagat ttcagatgca atagctacct atcaacaagc
    13561 cattaaaatc ctgaatacaa ttaaatcacc tatagaaagt gatctgcaaa ttgttcagga
    13621 gctaacgcaa catataagat acttacaaaa taaacctagc tctacaaaag atcaattttc
    13681 atcctgttat aaagaggctc aaactcctgt aattgccact gcttcagagg tgggattttt
    13741 taattacagg ccacaagagg cttttcaggt atcggattca tttcatagcc ctaatgaaat
    13801 ggatgtggaa tactgtgatg tcgatttgcg cccataagta cgttgctagt tgagtgacga
    13861 taattatttt tgtgttcaaa cggcagatag gtaggatttt agggtccttg ccttttgcag
    13921 caatattgca aaaaactacc aaatttagaa accactgcat ctcaagtata attaattctt
    13981 gtaacttgtg attgcagtgc agcaagaatt tgcgtggcat agggtgttgc gccctttgtt
    14041 ccatgaatta actacttatg gtgatttaaa ttgggacgtt ggctaagcgt gtcctcttca
    14101 cgccaaaaag tgtttgttta atcagcaaac ctccattatc ggggtaaagt attattttat
    14161 cttcaataat ttacactttg atatttttct tgcaacctgg ccgtgataac atcatgcacg
    14221 aacagaattt tcattaggga cagatagtta catgaacaag ggaatgaacg catttgtatt
    14281 cctgatcgcg gtaattgccg gatttggcgg ttttttattc ggatttgatt ccagcgtcat
    14341 agctgatgtg aaagatcagg ttatctccca attggcatta acagactggc aatgggcgca
    14401 agttgtcagc agtagtttat tgggatgtat attgggaata cccataagtg gtcttgtcgc
    14461 agataagtta agcagacgat ccttattaaa agttgtagct ttaggcttta tactggggag
    14521 tagtttgtgt gctttggctc ccggcttcgt ttcgatatta ctcggtcgat ttattatcgg
    14581 catttgcata ggtattgcct cctatattgc tcctttattc attgcagaaa tcgcgccgcc
    14641 tcatcagagg ggaactttag tattgattaa tggactaacc atcacgtttg gtcaggctat
    14701 cgcctattta attggctatt ttctccatga ctattcttta atgagttgga gatatttatt
    14761 ttggatagga agtctacctg ctctcgtttt attttcagga atgtattttg tccctcattc
    14821 accccgctgg ataatgatta aatatggagc tgatgctact ttaaagacct taaagcaaat
    14881 taggccagtt gggtataata ttcagcagga attgactgaa attagtaata atttagttaa
    14941 tcctgcacca gcctatatcc agatgtttaa aaaaccaatt gtttttgttc tcttattagg
    15001 agttggttta ggactattcc agcaattttc cgggatcaat gctttgatgt attatggacc
    15061 tgttgtcttt gaatcatttg ggttttctcc agttaaaaat gcaattctcg ctaccttttt
    15121 tttgggtctg gtcaatttta tttttacagt ggttaccctt ttttatgttg ataaattagg
    15181 cagaaggttt ttgttaatca gtggtacttt gatcgcatca attagcttat attttgttag
    15241 cttactatcg tgccactctt tatttgtcaa taaattctgg attttaggtg gtttatcatt
    15301 ttatgtgatg gggtattgta ttagcctggg gtctctgttc tgggttttaa tatccgaaat
    15361 atacccctta tcagtgcgtg gtttggcaat gagcattgct actatgtttc aatggggcgc
    15421 taattttgtt gtatcacttt tgtttcttcc catttatcaa ttctcaggtc aaacagttac
    15481 ctttgctatg ttcgctacct tttgcttgtt ggcatgctgg tttatttatt attttgtccc
    15541 ggaaactaca tcagtatcgt tggaaaaact ggaagaaaat ttaagctctg gaaaaaaagt
    15601 aagagatata ggtcaaccag tatcgaaaaa tataaaaata gcaaaattta atttaattat
    15661 ggattaatca tgaaaaagaa aatatgtcga attgggatag taggtgcggg aagaattggt
    15721 agactgcacg cagaaaatat tctatttcat ttaccgcaat ttgaattagt ggccatagca
    15781 gatccccaga tcgataggaa ttgggcagaa aaattaggga ttgcaatcgc gtctactcaa
    15841 tcagaagatg tgctatgcca ccctgacttg gatgccatct tgatcgcgtc accatctcat
    15901 ttacatctcg aacaaattat tacagcgagt attcatgaga aagcgatttt ttgtgaaaaa
    15961 ccaattggtt tgaatgaaga agagatagag aaaacattaa acataatcaa aaacaataat
    16021 actttattgc aacttggatt caatcgtcga tttgatccag gttttgccaa cattcagaaa
    16081 agagtgcagt ttggtgaaat tggcactcct catattcttc gaattacttc tcgtgatccg
    16141 gcttgtccat ccaaagaata ttgcgcgaca tctggtggaa tatttatgga catgtctatt
    16201 catgattttg atatggcacg ttttttaatg caatccgagg ttgtggaggt ttatgcaaca
    16261 ggggctgttc taattaatcc tgattttgag cagtttgatg atgtagatac agcaatcatt
    16321 caactacggt tcgctaatgg cgcatttggt gtcatagata acagccgtca agcaatctat
    16381 gggtatgatc aaaggataga agtattcggt tcaggtggta tgttattggc tgaaaatcag
    16441 tttgagcata cggtatctcg atactcggct aatggaacgg atggcgcaaa acctcaatat
    16501 tttttccttg agcgttatca tcaagcttat ctggcagaac tgaatgcgtt ttatagtgcc
    16561 tggaatgata atcagacaag tcccgtttca ggattggatg gactacaagc cttgcgcatc
    16621 gcaaaagcag cgaaacaatc tttagaaacg catctgccag tagtattgag ttgaggccgc
    16681 tatgaaacag tttcccggat tttattttga taagaataga gcaatcgatt tgatttgtat
    16741 gggacgcgta gcagttgatc tctacgcgga gcaaatagga ttggatttaa aggatgttgc
    16801 aagctttaaa aaatatttgg gtggttgtgc aggaaatatt gccgttggag ctgcccgatt
    16861 aggattaaaa agccagatgt tttcctgtgt tggctcagat gagttaggta agtttctgaa
    16921 agaagaactg gaacgtgagg gagttaatat tgagttatta ttggaaacag ataatcattt
    16981 aactggcctt gtacttttgg gtattaagcc tccctccaat ttccctctgc ttttttatcg
    17041 taatgattgc gcagatatgc agattaaatc agatcaaatt agttttactg atttaaaaaa
    17101 tgcaaagtca ttattgataa cgggaacagg tttatccaca gaatcaatga aaaatactac
    17161 cctggaagta gttcatttag cccgaaaagc agaaacgaca atagtttttg atctcgatta
    17221 tagacctgtt ctttggcggt taactgtgat tggtaatggg gaaagtcgtt attgccagaa
    17281 tgaatatgta actcaagtgt atcaacaaat tttgccttta tgcgatttga ttgttgggac
    17341 tgaagaggaa atatgtattg caggcggttc gaatgatatt gaagtttcat taatcaatat
    17401 caggcagcga accgatgcgc caatagttgt taaattagga gagaagggtg ctcgtgttca
    17461 ttttggagca gacaaaggtc tattacaggc aaaatctttt cctgttgaag tattgaatgt
    17521 tttgggagca ggtgatggtt ttatgtccgg gcttttagct ggtttgctgc aaggaaaatc
    17581 atgggagcaa gcagtaacct atgctaatgc ttgtggcgcc ttggtcgtga ctcgtcatgg
    17641 ttgcgctcct gcaattccat acaaggaaga attgaactat tttattcaag aatatgatca
    17701 tgagcctgaa atatggaaca gctcccagtt aacacaattg catgagaaat taagtaaaaa
    17761 tcgaaagaca cagttgctta ttaaaaatcc atgtggattt gccgatggtt taaattcgat
    17821 tgtaacgatg caagactcac ctacagcaat gagttttagt tcccttaaat taaataaggg
    17881 gcaaagccac caatttaact cgagttatga atttgctgcc ctcttaatga caggaaaagt
    17941 tgttttccag tatggctgtt atagccagga ggttgaacgt acagattatt tttctcaatc
    18001 accctatgtc ttgcactgct caaagaatga agtaagttca gtaatcgctc tgactgattg
    18061 tgaaatttta ttaatggaaa cggaaaacga aaatttattt tctccgattt tatttgatgc
    18121 atctaatatg cttgagttag acaatagggg agaaggagtt cttgaaaata cttcatatcg
    18181 acttgtcaga acggtatttg ataaacgaaa cagacccgaa tctaatttgg tagttgggga
    18241 aattattacg tttcaagggc gttggtcaag ttatccttca cattatcatc aacaacctga
    18301 aatttaccac tacaggtttt cagagcctca aggctatgct tttggcgaaa atggtaaaga
    18361 ggtgatgcga attgaacact atgataccta ccagattgcc aataatcagg aacatgctca
    18421 ttgtacagcg cccggttatg cactttatac actgtggttt atccggcatt taaataataa
    18481 cgcatatacc ttgcctacct tcacaaaaga ccatgaatgg acaaggacaa gcagggcaaa
    18541 tttaagaaca tggcaacata aacagtagta atccttatct gcagtatgat tttgagagtc
    18601 aaataaatga agattaaatt aactatggct caggccctat taagattttt agctaatcag
    18661 tatgtcgttc tggataataa tgaataccgt ttcatcaatg gtgtatttgg aatatttggc
    18721 catggtaatg taaccggatt aggtgaggca ttggaatacg atgctcaggg tttaatttat
    18781 tatcagggac ataatgaaca aggaatggct cacgcagcaa ccgcctatgc caaacaaaaa
    18841 aatcggctag ggataatggc ttgtacttca tcaattggtc ctggcgctac taatatgatt
    18901 actgcagcgg cgacagcgac aaccaataga attccattac tgttgttgcc aggtgatgta
    18961 ttcagttgca ggcaaccaga tccggttctc caacaattgg aggtgccata tgactacacc
    19021 atatctgtta atgattgctt taaaccagta agtaagtttt gggacaggat aaccagacct
    19081 gaacagttaa tgacagcgtg tatgcatgct atgagggtat taaccgatcc agttgaaaca
    19141 ggtactgtta ctttatgttt gcctcaagac gttcagtctg aagcttatga atatgaggaa
    19201 tcttttttta aaaagcgctt atggcgtatt gagagagaga ggataagtga tgcaatgttg
    19261 acagaggcca taaggcatat cagaacggct aagcaaccat taatcattgc aggtggtggt
    19321 gtacattatt cactggctac ggagacttta tcagagtttg cattaaaaca tcgcattcct
    19381 gttgctgaaa cccaagcagg aaaaagtagc ctgacagcga aacaccccat gaacgttggt
    19441 ggaataggtg ttactggatc tgaaatagcg aattctctgg cagctcaggc tgatgttatt
    19501 ttggttatcg ggtctcgatt acaggatttt acgacagctt ccaaatgggc ttttaaaaac
    19561 caggactgcc aaatcattca tcttaatgta tcacgtcttg atgccatgaa aatgaatgga
    19621 gttatgttaa aaggagatgc taaagctggt ttggagcaat tggggaaaga gctggcagat
    19681 tatcaaacct ctctctctta tcagcagtta atacgtcatt atcaaaatga atgggctcaa
    19741 gagttagata aattgactca gtcttattct acagagcagg ctgggttaca ccaaactact
    19801 gttttgggac tgctcaatca gtatgttact gaagaggatg tgataattag tgcagcgggg
    19861 agtttgccag gtgatttgca tcgaatttgg caatccaaaa aggcaaagga ttatcatttg
    19921 gagtacgctt attcctgtat gggatatgag gttgcagcgg gtctaggagt acgtattgca
    19981 aaagaaaatc aggaaggaga ggtctatgtt ttagttggag atggcagttt tgtcatgatg
    20041 cattcagagc ttttaactgc catccaggaa cgcaagaaaa ttacaattct catttttgat
    20101 aaccatggat ttcagtgcat tagaaattta caggaaggga atggtagtct gggttttggt
    20161 aatgaatttc gttataggga gccaactact aatgctttaa atggcgatta tttacccata
    20221 gatttttgta agtacgcaga gggattgggg gcagctactt attttgttaa atcctatgag
    20281 caattccgga ctactttaga ctcagcaaaa aaacaggaaa atagtacggt tattgttctt
    20341 cctgtattac caaagaccat gagtcaagga tataagactt ggtggcgggt tggagttgct
    20401 gaggtatctc aatcggaatc agtgagtaaa gcttaccaaa tgatgcgaga gcaaattgaa
    20461 acagttagac aatattgaag atggaagtga agatgagtat tcaacttggt atcgcaccaa
    20521 ttaattggtg caatgatgat gatccgaatt taggtaaaga cattagtttt gagcaatgta
    20581 ttaacgaaat ggctatggca ggttattctg gcactgagct ggggaataaa tacccacgag
    20641 atgtaagaac gttaaaggct gctttggaac aacgaggttt acaattatcc agttcatggt
    20701 ttagcactta ttttactgag gccaatgctt ttgaaaaaac cgtatcaaga tttcttgaac
    20761 acttaagctt tatgcgcgca ttaggtgcat cgtatattaa tgtttgtgaa tgcggtcatg
    20821 ctattcaagg cactgattta ccgatattaa gtgctcatat ccctcaattt actcaagagc
    20881 aatggagttt attgattcaa gggctacata ccttaggccg aattgcttac gacttcaata
    20941 tgaagatcgt ttatcattac catgcaggga ctggggtgtt ttgggagaaa gacattgatt
    21001 ttttaatgac caataccagt ccaaagttgg tttctctatt acttgataca ggacatgctg
    21061 ccttttcagg aatagatccg gttaatttaa tagataagta cggtgatcgt attttgtatg
    21121 ttcatctgaa agacattcgc cctgaggtct tgagacgagt tgaaatggag agactcagct
    21181 ttatggatgc agtaagaaga ggggtattta ctgtgcctgg tgatggagca attgatttca
    21241 tacctatatg ggcagggctg caaaaacatc gctataatgg atggatgata gtggaggcgg
    21301 agcaggatcc ggaaaaagca catcctttgg aatacgccaa aaaagcatat gattatttgc
    21361 attcatttat tcattaaaga aaggctgatg attgaagatt taataaaatt tactttatta
    21421 aaaataccag ggaagcttaa gagttatcta tttataggct gagatctggt taagttcact
    21481 ataataagga gattatccat gtcaatgaag gataaattgg tacaatctgc cataagtgct
    21541 tttttagttc tcattgcatc aaacacagca atagcagagg atttgagtaa ggagaaatgt
    21601 tatggtgtgg ttaaaaaagg gatgaatgat tgttcaacag caacttcaga ttgtgctggg
    21661 tcttcaacca aggatagcca aaaggatgct tttattttat tacccaaagg gatttgcgaa
    21721 aaacttgttg gtggatcatt ggatatgggt aaataaaaca ttaatttaat ttgggagttg
    21781 aatatgaaac ggatttacag gcattttatt gctccaatat tggcagtctt tgtattgtcg
    21841 tcttatgtct acgcagaacc acaaaccttg accttagata atcagcatac ttatgtgtta
    21901 tggaagatca agcatttagg cttctcaact caagctggaa aatggtatgc aagcggtcaa
    21961 cttgttcttg ataaggataa cccgcagcag agtaaagtga atgttactat caaggtcgat
    22021 gatatcgtca caggtatacc cgagttggat aaacatttga aaggtaaatt attttttgat
    22081 accaagcagt ttccaacagc aacttttgtt agtaacagag tggaagtaat gggaaagaat
    22141 aaagcaaaag tatatggaat gcttactttg catggcgtca caaaacccat catattgaac
    22201 gtgatattaa ataaagcagg aattaattta ttaaatgaca gagaaacagc tgggtttagt
    22261 gctaccactt cattaaagcg ttctgattat ggaataaaag ctttaatacc agaagtagga
    22321 gatgatgttg aaatagaaat agaagcagaa gcctatcttg ataagaaata tggataatct
    22381 atgcaattaa aaaatacttc aaccagcttt ggtctggttg cgattttttt ccattggatt
    22441 atggccgctt taattattgg gctattggga gtgggtttat acatggtcag gatccctatt
    22501 agtcttgaga aattaaaatt atatggttgg cataaggagt acggatttct ggtattgttt
    22561 ttggcttttt tcagattgat ttggcgtttg actaatcaat taccggaatt agctattccc
    22621 ctgttagaga aaattgcagc tcgcagcatg cactgggctt tttatttttt catgtttgct
    22681 atgcccatca gtggatggct aattacctcg gctgcaggat tgcctgcttc tttttttggt
    22741 ttatttgttt tacctgacct tgttgcgccc gatgagaata gaagaatttt gttccaattg
    22801 attcatgaat ggttgggtta tgcattaatt atggccataa tcctgcatgc agcggcggca
    22861 ttaaaacatc attttattaa taaggatgat atattacgga gaatgttata gtgaatgtct
    22921 atattaaatc ttccctttgg gtattgatat tttttgtgaa tacattgatt tacgccagta
    22981 aactccctga atgggaaatc gtgccagcac aaagtgagat tacttttact ggtactcaaa
    23041 atggtgcccc ggttacaggg cgatttaaga aatttactgg tcaaattttt gctgatcctt
    23101 caaactacaa gacagggtca attgatatca ctattgatat gaattcgatt gcagcacctt
    23161 ttgaggacat cgtaacgaca ttggcctccc ctgactggtt caacgtcaag acatttccta
    23221 atgcagaatt taaagcttcg aaatttaatc aattggatga taaaacctat gaagcagaag
    23281 ggattctaac tataagagat aaatcagctc cggttactct gaagtttgtt gtggagcaaa
    23341 tttctaacga tcaggcttta gttgaaggta gctcaacaat caaacgcagt acatttgggg
    23401 taggacaagg tgaatgggct agtactgatg aaatacagga tgcagtaatt gttcgcttta
    23461 aaataagcgc tacacggaaa aaataattta aaggttaaca tgcaagcaat aatatgtaga
    23521 ggaatgttaa ataattttaa ttatttcaaa ttttaagaaa ctacatggca tcatgcattg
    23581 gccgggtttt ttcac
//
LOCUS       DACTJE010000034        23351 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.034, whole genome shotgun sequence.
ACCESSION   DACTJE010000034 DACTJE010000000
VERSION     DACTJE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 23351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23351
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.034"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(212..526)
                     /locus_tag="I9760_001036"
     CDS             complement(212..526)
                     /locus_tag="I9760_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HAT5042813.1"
                     /translation="MSHYTKHVFICTNQKAPGKQCCANSGGEEFFDFMKSKLLEFDLH
                     GPGKIRVSKSGCLGRCGSGPCIVIYPEGVWYTYSSFEDIEQIIKYHLIDGKIVAPLLL
                     DR"
     gene            complement(531..830)
                     /locus_tag="I9760_001037"
     CDS             complement(531..830)
                     /locus_tag="I9760_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAT5042814.1"
                     /translation="MIVNALSKAIMAETLCDELKLNKPVAKEMVENFFEELRHALENG
                     QHVKLSGFGNFTLRDKPQRPGRNPKTGEEIPVEARRVVTFKPGLKLKTKIEKIGK"
     gene            complement(827..3208)
                     /gene="pheT"
                     /locus_tag="I9760_001038"
     CDS             complement(827..3208)
                     /gene="pheT"
                     /locus_tag="I9760_001038"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAT5042815.1"
                     /translation="MKVSKLWLREWVNFSLTEQELAEQLTMAGLEVDAVNPVAGQFTH
                     VIVAEVLDTKPHPDADKLTLCEVNINKDKPLKIVCGAANVRPGLRVALAMIGAHLPGG
                     LQIKESKLRGELSQGMLCSATELGLAEHSEGIMELSDEAPIGMDLREYLALDDHVFDI
                     DLTPNRADCFSILGVAREVAVLNKLPLIEQPIVTVPPAIDDGLRVNLIHSEACPRYCG
                     RIIRNLNLEAKTPLWMAERLRRGGIRTLHPVVDVMNYVMLELGQPMHAFDLAKINGEI
                     NVRYGVSGEQLELLDGQEVVLNDNVLVIADKEKPLAMAGIMGGANSAVQEQTQHVFLE
                     SAYFNPVTIAGVARKYGLFSDSSQRFERGVDPCLQSKALERATELILSISGGEAGPVI
                     ESFDKKFLPGTVSFLFDTTKVKKLTGLSIPLNEMKNLLEGLGVVIIKETNHFFEVTIP
                     SHRVDLQQDADLVEEIIRLYGYDKLQAQPMQTSVQAGLISAKEKIATHVSSWFSAKGY
                     HETISYSFVDPELQEALYPQKEFMELLNPISSELSQMRAGMWPGLIASMIYNSHRQQT
                     AVKFFEIGVVFDLDGGQLKERSCIAGLLMGEQGNLNWSESARLFDFYDLKGDLQSLFA
                     SLKLDDVEFIQSSHHALHPGQSAQIVINGKHSGWIGVLHPRLSDAFDLDQDVVLFELN
                     LESLINPTIPLYKPISKYPQIRRDLSFLVDRQISAMQIERVIRNTVKEDWLKSFDVFD
                     VYMGKGIPEDKKSIAVAMTLQDDTRTLVDAEINLTISAIIKKLENEFSILLRE"
     gene            complement(3466..4479)
                     /gene="pheS"
                     /locus_tag="I9760_001039"
     CDS             complement(3466..4479)
                     /gene="pheS"
                     /locus_tag="I9760_001039"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAT5042816.1"
                     /translation="MVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLKG
                     LANLSAEEKPKVGQLVNQAKQGISALIETKMIELKEKQLLAKLAAEQIDVTLPGRNHS
                     TGSLHPVTQVKHRINDYFSRLGFDIVEGPEIETEFYNFEALNIPGHHPARAMHDTFYF
                     GDGRLLRTHTSPVQIRTMEQRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQA
                     TLAGLKGLLQDFFAYFFGRELALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFTG
                     WLEVLGCGMVHPNVLIAVNIDPNEYHGWAFGMGMDRLAMLYYGIDDLRMLFENDLTFL
                     RQF"
     gene            complement(4619..4978)
                     /gene="rplT"
                     /locus_tag="I9760_001040"
     CDS             complement(4619..4978)
                     /gene="rplT"
                     /locus_tag="I9760_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAT5042817.1"
                     /translation="MPRVKRGVTAKARHKKILDQAKGYYGARSRTYRVAKQAVIKAGQ
                     YAYRDRRQKKRQFRALWITRINAQARECGLSYSRLIDGLKKASIELDRKILADMAVHD
                     KVAFAAIAEQAKAALAG"
     gene            complement(4994..5194)
                     /gene="rpmI"
                     /locus_tag="I9760_001041"
     CDS             complement(4994..5194)
                     /gene="rpmI"
                     /locus_tag="I9760_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAT5042818.1"
                     /translation="MPKLKSHRGAAKRFRKTASGAIKRRGAYRNHILTKKSTKQKRHL
                     RVEAGTLKPCDARLAERMLHGS"
     gene            complement(5215..5751)
                     /gene="infC"
                     /locus_tag="I9760_001042"
     CDS             complement(5215..5751)
                     /gene="infC"
                     /locus_tag="I9760_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAT5042819.1"
                     /translation="MSAPTKRENDRARINEQITVPEVRLIDVDGNQAGIVSTREALRA
                     AEESGLDLVEISPTAKPPVCRIMDYGKFLFELSKKQAEARKKQKQIQVKELKFRPTTE
                     DGDYQVKLRNLIRFLNHGDKVKITLRFRGREMAHQELGMKILERLQADTADVAIVEQH
                     AKREGRQLLMVLSPKKTK"
     gene            complement(5776..7689)
                     /gene="thrS"
                     /locus_tag="I9760_001043"
     CDS             complement(5776..7689)
                     /gene="thrS"
                     /locus_tag="I9760_001043"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAT5042820.1"
                     /translation="MPNVKLPDGNVKHFEAPLTIYDVAHHISPGLAKAAIAGRVDGVL
                     VDTSYLIKEDCSLIIVTEKHEDSLEIIRHSTAHLLAQAVKALFPSAQVTIGPVIEDGF
                     YYDFAFERSFTPDDLSLIEAKMHELAKANLSITRRELPRNEAIQYFKDLGEEYKAKII
                     ADIPENEALSLYRQGDFEDLCRGPHVPSTGFLKAFKLTKVAGAYWRGDSNNEMLQRIY
                     GTAWADKKSLEEYLFRLEEAEKRDHRKLGKALDLFHFQDIAPGMVFWHPKGWTIYQEL
                     EHYMRNRLVDFGYQEIRTPQLVDRSLWEKSGHWANFRDEMFVTETENRHYAVKPMSCP
                     CHVQIYNHGLKSYRDLPLRLSEFGNCHRCEPSGALHGLMRVRNMVQDDAHIFCTEDQI
                     QSEVAMMLELVQSVYKDFGFTEIKYRLALRPEKRVGSDDVWDKAETALKLAMQGRNIE
                     WVDAPGEGAFYGPKIECSLSDCLGRIWQCGTIQVDFSMPARLEASYVAEDGSKQTPVM
                     LHRAILGSFERFMGILIEHYAGKLPLWLSPVQAIVLTISEKQNEYAEKVRKTLQKRGI
                     RANFDLRNEKIGFKIREHTLQKIPYLLVVGDKEVENCQVAVRTRDGIDLGVMTIDTIC
                     DTLTQEIIRKGSI"
     gene            complement(7818..7892)
                     /locus_tag="I9760_001044"
     tRNA            complement(7818..7892)
                     /locus_tag="I9760_001044"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7858..7860),aa:Val,seq:gac)
     gene            8202..8489
                     /locus_tag="I9760_001045"
     CDS             8202..8489
                     /locus_tag="I9760_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HAT5042821.1"
                     /translation="MWFKIIHSHVEIAIYAKPNAKKSKLMAISDDRLHIALHAKPQEG
                     EANNELLFFISQFFKIPKTQIELIKGKSSRHKLIRLPLSESVFCFLNNPKI"
     gene            8812..9297
                     /locus_tag="I9760_001046"
     CDS             8812..9297
                     /locus_tag="I9760_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5042822.1"
                     /translation="MEKVNLTKQFAYRLRDAMIAAGHNSQRSPSGVCIHKLAEMTGYS
                     LQICRKYLRGEVIPDPVKLIEIASKLNVSPGWLLFGETHNDPGTSKEHITISKSLLHH
                     IFTKAACLYNRELVEDDVSDFLMDLINDISLINATEEQSKKIIDLAISSVSHFRHSQR
                     T"
     gene            complement(10007..11569)
                     /gene="wipA"
                     /locus_tag="I9760_001047"
     CDS             complement(10007..11569)
                     /gene="wipA"
                     /locus_tag="I9760_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector WipA"
                     /protein_id="HAT5042823.1"
                     /translation="MPKRLINKNIDIYNYPNEFEDNLGSISLGDLHGNAIKLIHFLFR
                     HKIIKFKTEIINFHEAYQQFVTIYEQYDDMVQEYLEIRTLLQLIQIKITNAQQRILDI
                     EQKLSLATDHQKEFSQSLLQLKKPIEANLQMAEKSKAGLEEKLSGLKTRLPSCIERFN
                     KFMTQIEINDIKTLIRLLGDEVADRGSCDYFTLRILDFLYQNQIAIKIILSNHGYEFI
                     HAYEKLVVGQPFKPKGYIGDIQIKSFWGLQLLLEQSVITEEELRSLVERAYKPTLKII
                     DYSLSEDGITLYSHAPIRFDSIRMAASQLGVTYNDSTKEALAETIDQLNAQLQIYMKN
                     NMLHLLFENNEINDPTNMTDEERNASPLIYLVWNRWNESKEVENARPGKYNGYFVTYV
                     HGHDPFQSPLTYVYNLDTLCGKYSRVGEEEQINKAFQFLTENRHTNVDKTASESLLRN
                     ISRYKVLDSDEYTLKHKIPKTSLELATDILDCKIKESLIKLSLLGKPKAVSGSLSVDQ
                     NISIPNQASLGK"
     gene            11850..13001
                     /locus_tag="I9760_001048"
     CDS             11850..13001
                     /locus_tag="I9760_001048"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAT5042824.1"
                     /translation="MRLLSFKKSKIVSIGTELEFQIIDCSSLSLVSRSKELMRALKDM
                     RYRDQIKPEITQSMIEINSSIHQSAKEMYDELLELQKILVETAASIDIAFCGGGTHPF
                     QQWTMQKIFPSKRFKKKFNQYRYLSKRATVFGQHIHIGCPTGDDAIYLTHALARYVPH
                     FIAISASSPFYLGINTNYCSSRSTIFNAFPLSGVIPYLRNWQEFSDYYRKMYRWKIIE
                     NMKDFYWDIRPKPELGTIEIRVCDTPLTLRKSILITAYIQALALYLLEEKSVQLSHDL
                     YYVYNYNRFQASRHGLEGELTVTDKDRPIPIMDDILETIKKIEQYINGLGNSEYIEEL
                     YSDVINKQNDSVLINKIYKQDGSFSKLVAAQCELWLSDSKDRKWMTQPS"
     gene            12980..14011
                     /locus_tag="I9760_001049"
     CDS             12980..14011
                     /locus_tag="I9760_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HAT5042825.1"
                     /translation="MDDTTFLSTKEKILEIKPLMESAFGNILGYQELENMLAICTLVP
                     FSNNQILFHQGDRIKGIYIIIKGTVDLIARTLGKGITKIETCLASDFLGETSFLLNEP
                     SATSAVAKDNVTCLFIPSAYFELMPAFSPEAKYKIMKAIGYQACGRLKQLHDKIIDFI
                     SHSDMQSLSFLGRVIQTFTQPKIHTLDESGVDKDQIYKKTLFNSFTQDETDELLEHAI
                     PLTANKNCVLIHENETIPVCYIVIYGAVQSNVIQNSKIAKLSVIGPDSLFASTACFLS
                     DTKFSITFITCESATLLKISEAELQYFQKNKPMIWYKLFNLICGSIISLARSLKKLDV
                     RLNIEKYNQ"
     gene            14018..14935
                     /locus_tag="I9760_001050"
     CDS             14018..14935
                     /locus_tag="I9760_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAT5042826.1"
                     /translation="MCRILSYLGQPTVVEELLYKPDNSFIKQSYHPKYLPYLLNLAGF
                     GMAAWDRNSINPLQAYVYKTSKLPFYDENLHNLAEKIAPHCLLAHLRGVSYHDKQVVT
                     DQNVHPFLYPVTSIALAHNGMLHNFETMRYDLLKYVKTEYRKYIKGTTDSEWIYAVFL
                     SQLENPKGHFDASEIINAVIATLKILKTVRQQNNIVINSPVNLFLTNGDFIAATRFCL
                     DYGWKPKDATQSTHFTYHSLWYTYGERYGFYENEYKMKGGTINSSIIIASEPLTENTT
                     TWLEVPEYTLIFANQVDGEIKITSQDINI"
     gene            complement(15063..16118)
                     /locus_tag="I9760_001051"
     CDS             complement(15063..16118)
                     /locus_tag="I9760_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HAT5042827.1"
                     /translation="MVNSDLILSPYQLNDKITLKNRVIMAPMTRNMANDDFSPTRQMA
                     DYYARRADAGLIITEGTIISPDGQGYSNAPGIFTQTHIDGWKMVTDAVHERGGKIFLQ
                     IWHVGRVSHPIFLHGNLPISASETMMTGKVRRADDLYYGKSRAATLEEINELIQNYAL
                     AARRAMIAGFDGIEIHGANGYLIDQFLHYHTNQRHDQYGQYPENMGRFALEVVKACIN
                     EIGGQSVGLRLSPGAYLNEIVGDERDAMVFRYVLEELNHYGIAYVHTGNFDDGIQFKE
                     LGFKTMTEFMRAHFKGTLIASGSYTLHQARKDIQNKKYDLIALGRPFIANPDLMNRIK
                     WNADFLPYENRMLDTLF"
     gene            complement(16190..16489)
                     /locus_tag="I9760_001052"
     CDS             complement(16190..16489)
                     /locus_tag="I9760_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5042828.1"
                     /translation="MNVINILKALSNEKRLQIIRWLKEPDKHFSSSHCDVSKDGVCVG
                     LIEKKSGLSQSTVSQYLSQLQQAGLIFTERRGQWTYCKANASLINEFINELKVMI"
     gene            complement(16742..17086)
                     /locus_tag="I9760_001053"
     CDS             complement(16742..17086)
                     /locus_tag="I9760_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042829.1"
                     /translation="MNNTIDNQSQEELTHFDANTPQSIIQLDRKLREMKSEVDMKIND
                     PSSYNGDDKEVYFQKLSKLSEEINEAIKSLDALVKMVDIQDEEFKKDFYESDVMKEFN
                     ESVMKSFDKLPE"
     gene            17203..17832
                     /locus_tag="I9760_001054"
     CDS             17203..17832
                     /locus_tag="I9760_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAT5042830.1"
                     /translation="MLLHYLFIVGICVEAITGALAAGRKKMDFFGVLLIASITALGGG
                     TVRDTLFNTYPLTWVAHPEYLLYTSVCAFLTIFIAHSLVRIMKVFLILDALGLSTFVI
                     IGTQKVLAHGMPPSVAVISGMITGICGGMLRDILCNDIPLVLRKELYAVIALAGALLF
                     ITLDYFHIPHNLNIIITLLCIFTARLLAIFFHIEIPIFDYSESIKRRKK"
     gene            complement(18040..18534)
                     /locus_tag="I9760_001055"
     CDS             complement(18040..18534)
                     /locus_tag="I9760_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAT5042831.1"
                     /translation="MVLISTSMGDIHIKLDTENTPLTAENFLNYVRKGFYNDTIFHRV
                     IDGFMIQGGGLNTNMEQKTTASPIQNEAKGAKPNKRGTIAMARTMDPHSATAQFFINV
                     ADNGFLNYSGDHPQGYGYCVFGEVVEGMDVVDAIAKVKTGQRNGHADVPVEEISIIEV
                     RELA"
     gene            complement(18561..19712)
                     /gene="tgt"
                     /locus_tag="I9760_001056"
     CDS             complement(18561..19712)
                     /gene="tgt"
                     /locus_tag="I9760_001056"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAT5042832.1"
                     /translation="MNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVN
                     NMMPSELSAAGTQIILGGNTYHMLCAPGMKVIENAGGMHRFMGWHGPMLTDSGGFQVF
                     SLSKNKEICSIDESGAHFKLPGSKGIIHMTPEMSLETQKIIGADIIMAFDQCTPDSCS
                     KDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSA
                     MNTDGIAIGGETVGFDMDTTVEVIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGID
                     MFDCVAPTRNARHGALYCGKLVREGNWLKFQSEHENGRIQIKKSCFADDLAPVMESCS
                     CYTCLNYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHLIINS"
     gene            complement(19774..20265)
                     /locus_tag="I9760_001057"
     CDS             complement(19774..20265)
                     /locus_tag="I9760_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HAT5042833.1"
                     /translation="MKKLTYRKIQSFQVIITLLVIFASFYLEYAVGLQPCPLCLMQRI
                     CVFILLGLMGLSLGTLKKAHIVSLMQFLVACAGLYFSLRQLWLQSLPSDQAPACMPGL
                     DVLIQYFPWQTVAKALFWGAGDCAEVTWTMFGISMPGWAAMYFLFMAIMGLFLFFRTR
                     TIN"
     gene            complement(20262..20690)
                     /locus_tag="I9760_001058"
     CDS             complement(20262..20690)
                     /locus_tag="I9760_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HAT5042834.1"
                     /translation="MRLNIALILSFFSIMLFAMDEVDEQQILQRIQPVGKVRVQEEQG
                     NNIEAGATKTTVVDSKSKKEPGQDIYDKYCSVCHRDGLAGAPKFQSEADWKPRLASKK
                     IDDLLASAIKGLNAMPAKGTCMDCSDAELKAAIQYMLPKS"
     gene            20879..22129
                     /locus_tag="I9760_001059"
     CDS             20879..22129
                     /locus_tag="I9760_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing autoinducer synthase"
                     /protein_id="HAT5042835.1"
                     /translation="MSNTEYQTIDNKTQYINGKEKHQNHAYPEYINHSMEKYYEGRVN
                     KSWQGKHILKGKTPTANSLIFSSNDYLNISQHPQLIKAQIAAMQKYGNGQMQSAVFLN
                     NSSFLLSQCEKKFSSFLNYSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWS
                     GVKAARAKSIPFLHNSVDSLKKRIEHHGPGIIAVDSIYSTLGTISPLADYADIARKFK
                     CLLIVDESHSLGTHGSQGKGMVAKLGLSSQIDIITASLAKALSGRGGIITGDRQLIEY
                     IRYSSLPSIFSSALMPHDLAGFSASLEIITQEEWRRAKLQTNADFLREALSCEGINIG
                     SSGSQIVPLVTGSEANTLWLRDELEKEDIFGAVFCSPATPKNKCLIRLSISAHHEKSD
                     LLRVIECLVRLARKRPDIPLFKGN"
     gene            complement(22211..23236)
                     /locus_tag="I9760_001060"
     CDS             complement(22211..23236)
                     /locus_tag="I9760_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT5042836.1"
                     /translation="MQHFSIPTCYFPSTAVFVDDSRDFLLNFVLQLDEGLAYRVFDSP
                     FEALDCIKQKRCELELLSERCLSEYTEAKNCPLTNHTINLDLAAIHAEVYNSRRFSEI
                     SVVVVDYAMPGMDGLEFCRRIEDTNIKKILLTGQADEKLAIAAFNEGLIHRYIKKSDP
                     DVASLITQSIHDLQLQYFQSMSDMIVRMLSVTSPNCLHDKKYAELFWRLCREKGIVEF
                     YLADNSGSFLMLDDDANISFLIVKNEADMQLHYDLALDNGASGDVLDQLHNGEKIPCF
                     WQSNLVTPQWNEWSNCLVPANRFVSDETYFYAYVQGAVLFDVRLDKILSYHQYLEELD
                     AEEMFLN"
BASE COUNT         7243 a         4971 c         3919 g         7218 t
ORIGIN      
        1 aacatcagat taatgactaa atgtgaagta ttttttatct tattgacgta agttcatact
       61 gaatcaacag aaaaccccga tactaatttt catctctttg tttcattaga agagactggg
      121 cctgactact cgctcagaat taaccaacgc ttaattttat caaaagcctt ttcgtgccag
      181 gaaagcacaa aaagtttatt tccctaaata attatctgtc tagcagaagt ggcgctacta
      241 tcttaccatc aattaagtgg tacttgatga tttgttcaat atcttcaaag gaagaataag
      301 tataccaaac tccttctgga taaataacga tgcaaggacc agagccacat cgtccaaggc
      361 atcctgattt gctgacccgg atttttccag ggccatgcaa atcaaattct aacaattttg
      421 atttcataaa atcaaaaaat tcttctccac cggaattagc acaacattgc ttacccggtg
      481 ctttctgatt tgtgcatatg aatacatgct tggtgtaatg agacaacaaa ctactttcct
      541 attttttcaa ttttcgtttt taattttaaa cctggcttaa aggtaacaac ccttctggct
      601 tccacaggaa tttcttcacc tgtttttgga ttgcggcctg gcctttgtgg tttatctctc
      661 aaagtaaaat taccaaaacc ggataatttc acatgctgac cattttcaag agcatggcgt
      721 aattcttcaa aaaaattttc aaccatttct ttagcaactg gtttatttaa ttttaattcg
      781 tcacacaacg tttctgccat tattgctttg cttagtgcgt tcacgatcat tccctcaaaa
      841 ggattgaaaa ttcattttcc agtttcttga ttatagcact aattgttaaa ttgatctcgg
      901 catcaactaa agttcgagta tcgtcttgta gtgtcatagc cacagcaata ctttttttat
      961 cttctggaat acctttaccc atatacacgt caaagacgtc aaacgatttt aaccaatcct
     1021 ctttaactgt atttctgatt actcgctcaa tctgcatggc acttatctgc ctatcaacca
     1081 ggaaagacaa atcgcgcctt atttgtggat atttggaaat aggcttatac agaggaatag
     1141 tcggattgat taatgattca agattcaact caaacaaaac cacatcctgg tcaagatcaa
     1201 aggcatccga caagcgagga tgcagcacac cgatccagcc tgaatgcttg ccgttaatca
     1261 caatctgagc tgactggccg gggtgaagcg catgatgaga tgattgaata aattcaacat
     1321 catccagctt caatgaagca aacaaggatt gtaaatcccc ttttaaatca taaaaatcaa
     1381 ataaacgggc agattcactc caattcaaat tgccttgctc ccccattaac aagccagcaa
     1441 tacatgaacg ctcctttaat tgccctccgt ccagatcaaa aaccacgcct atttcaaaaa
     1501 atttcactgc ggtttgttgt ctgtggctat tataaatcat agatgcaatt aagcctggcc
     1561 acattcccgc acgcatttgc gataattcag aggaaattgg attaagcagt tccataaact
     1621 ctttttgtgg gtaaagcgct tcctgcaact caggatcgac aaaactataa ctgatggttt
     1681 catggtagcc tttggcacta aaccacgatg aaacgtgtgt cgcaattttt tccttggcac
     1741 tgattaaacc agcttgtact gatgtttgca ttggctgagc ttgtaacttg tcatagccat
     1801 ataaacgaat aatttcttca accaaatcag catcttgttg caaatcgact cgatgagagg
     1861 gaatggtgac ctcaaaaaaa tgattagttt ctttgatgat gacaacacct agaccttcca
     1921 gtaaattttt catttcgttt agagggatag acagcccggt taactttttc acttttgttg
     1981 tatcaaataa aaaggaaacc gtacctggca aaaacttttt gtcaaaagac tctatcactg
     2041 gcccagcttc gccacctgag atagataaaa tcaattccgt cgctctttca agagcttttg
     2101 attgcaaaca agggtccact ccccgctcga atcgttgtga cgaatcactg aataagccat
     2161 attttctggc aactccggca attgtcaccg gattaaaata ggcgctttcc aggaaaacat
     2221 gctgtgtctg ctcttgaact gcgctattag ctcctcccat tattccggcc atagccaaag
     2281 gcttttcttt atcggcgatt accaacacat tgtcgtttaa tacaacttct tgaccatcca
     2341 ataattctaa ttgctcgccc gaaacgccat aacgtacatt aatttcacca ttaatttttg
     2401 ctaaatcaaa agcatgcatg ggttgtccaa gctcgagcat gacataattc atcacatcga
     2461 ctacagggtg caacgttcta atgccgcctc tacgtaagcg ctcagccatc cataatggtg
     2521 ttttcgcttc aagatttaaa ttacggataa ttcgaccgca atatcggggg catgcctcgg
     2581 aatgaattaa attcactcga agaccatcat cgatggctgg cggcactgta actatgggtt
     2641 gctctattaa gggtaattta ttgagaacag ccacttcccg cgcgacaccc aaaatactaa
     2701 agcaatctgc gcgattcgga gtcaaatcaa tatcaaatac atggtcatca agggcaagat
     2761 actcgcgtaa atccattcca attggtgcct catcactcag ttccattatc ccctcggaat
     2821 gttctgccag tcccagttct gtcgcagaac acaacatgcc ctgagacaat tctccacgca
     2881 atttggattc cttaatttgc aaaccgccag gcaagtgtgc cccaatcatg gccagggcaa
     2941 ctctcaaacc aggcctcaca ttagccgcac cacaaacaat tttcaacggc ttgtctttat
     3001 tgatattcac ttcacataag gttaatttat cagcatctgg atgaggcttg gtatctagga
     3061 cttcagcaac aatgacatga gtgaattggc cagctacagg attcactgcg tcgacttcca
     3121 agccagccat ggtcaattgc tcagccagtt cttgttcagt taatgagaag tttacccatt
     3181 ctcgtaacca taacttactt actttcattt aaagtcctca tctctgtaac tgttcatttg
     3241 gctcaaagtt tccatccgtt aattttgctc tctttatgat aatagagata aaattactga
     3301 taactatttg cctgtatcaa ctacataata aaatattatt ttcagtattc atgggaatta
     3361 tcccggacta caatttggtc taatctgtat catttatgta ccatatgaaa gacaatcata
     3421 aagaaacata aaaataaaat ccgggactca ttatttttat aatggttaaa actgccgtaa
     3481 aaaagttaaa tcattttcaa ataacatacg caaatcatct atgccataat aaagcattgc
     3541 caatctgtcc atacccatgc caaaagccca tccatgatac tcattcggat caatgttcac
     3601 tgcgattaaa acgttagggt ggaccatacc acaacccaat acctcaagcc agcctgtaaa
     3661 cttgcaagag cggcaaccct taccattaca ttgagtacat tctatatcca cttctgcgga
     3721 aggttcggta aaagggaaat aggaaggtct gaatcttaat gccaattccc gaccaaaaaa
     3781 ataagcgaag aaatcttgca acaaaccctt taacccggcc aaagtggctt gcttatcaat
     3841 gagtaatccc tccacttgat gaaacatagg ggtatgggtt acatccgaat cacaacgata
     3901 aactcggcca ggagcaatta aacgaaaagg tggcttacgc tgctccattg tcctgatttg
     3961 tacaggagag gtgtgtgtcc tcaacaagcg tccgtctcca aaataaaacg tatcgtgcat
     4021 cgcacgtgca ggatgatggc cagggatatt taatgcttca aaattataaa attctgtttc
     4081 aatttcagga ccttcaacaa tatcaaatcc taaacgacta aaataatcat taattctatg
     4141 ctttacctga gtaacaggat gtagggaacc tgtggaatga ttgcgaccgg gaagagtaac
     4201 gtcgatttgc tctgctgcca gcttggctaa taattgcttt tctttcaact cgatcatttt
     4261 tgtttcaatc aaagcactaa ttccctgctt tgcttgattg accaattgac ctaccttggg
     4321 tttttcctct gcagacaaat tagcaagtcc ttttagcaat tcggtgagct tgcctttctt
     4381 gcccaaaaaa tcaacacgaa tttgttcaag agcaactata tccgtggctt gtttgattgc
     4441 ttctgaagct tgttcttgta tggtgtttat aagtaccaac atagttctac ccacaagtaa
     4501 aaaggaggcc ttagcctcct caataatttt tattttacaa accggctttt atttaagatt
     4561 gctattccga attgagattt ataaacgaaa ggcaataaca aaaaaagcag gctgaaaatt
     4621 aaccagctaa agctgctttg gcttgttctg caatagcagc aaaggcaact ttatcatgaa
     4681 ctgccatatc agccaggatt ttcctgtcca gctcaatgga agcttttttc aaaccatcaa
     4741 tcaagcggct gtaggataac ccacattcac gagcttgagc attgatacga gtaatccata
     4801 aagcacggaa ttgtcgcttt ttctgccttc tgtctcggta tgcgtattga ccagctttaa
     4861 ttacagcctg tttagcaact cgataggtac ggctacgggc accataataa cctttggcct
     4921 ggtctaaaat tttcttgtga cgcgcttttg ctgtcacacc acgttttact cttggcattg
     4981 ttcaatctcc ccgttaacta ccgtgcaaca tacgttcagc caaacgcgca tcgcatggct
     5041 tcaaagtacc tgcttctaca cgcaaatgac gcttttgctt agtagatttt ttagtgagga
     5101 tatgatttct gtaagcgccg cgacgtttaa tcgctccact cgctgtcttt cggaagcgtt
     5161 tagctgctcc acgatgtgac tttaacttcg gcataacaat atactccgca ttcgttactt
     5221 ggtttttttg ggtgataata ccatcagtaa ttgtcttcct tcacgttttg catgctgctc
     5281 cactatagct acatcagccg tgtcggcttg caaacgttcc aaaatcttca taccgagttc
     5341 ttgatgagcc atttcacgac cgcgaaaacg caatgtgatt ttgaccttat caccatgatt
     5401 gaggaaacgg atcaggttgc gtagcttgac ctgataatct ccatcttccg tcgtaggacg
     5461 gaattttaat tctttgacct gaatttgttt ttgcttcttt cttgcttcag cttgtttttt
     5521 actcagttca aagagaaact taccataatc cataatacga caaacagggg gtttggctgt
     5581 tggagatatt tccaccaaat ccaatccgct ttcttctgct gcccttaacg cttctcgcgt
     5641 tgatacaatt cctgcctggt taccatcgac atcaattaag cgtacctcag gtacagtgat
     5701 ctgttcatta attcgtgcgc gatcattttc acgcttagtt ggtgcactaa tggtttaatc
     5761 ctccagttcg tttatttata ttgatccttt acggataatt tcttgcgtta aggtatcaca
     5821 aattgtatct attgtcatca cacccaggtc aatgccatct cgagtgcgaa cagctacctg
     5881 acaattttca acctctttat cgccaacaac caataaataa ggaatttttt gtaaagtatg
     5941 ctcgcgaatt ttaaatccaa tcttttcatt tctcaagtca aaattggcgc gaatccctct
     6001 tttttgcaaa gttttcctca ctttttcggc atactcattc tgcttctcac taatagtaag
     6061 cacaatcgct tgcactgggg ataaccataa tggcaattta cctgcataat gttcgattaa
     6121 tattcccata aatcgttcga aagaacccaa aatggctcta tgcaacatca ccggtgtttg
     6181 cttgcttcca tcttcagcaa catagcttgc ttcaaggcgt gcaggcatag agaaatccac
     6241 ttgaatcgtt ccgcactgcc aaattctacc taaacaatca gagagagagc attcaatttt
     6301 aggtccataa aatgctcctt ctccaggcgc atccacccac tcaatatttc ttccctgcat
     6361 agccagttta agggcggttt ccgccttatc ccaaacgtca tctgaaccga ctcgtttttc
     6421 agggcgcaaa gccaatcggt attttatttc agtaaaccca aagtccttat atacggattg
     6481 cactaactcc agcatcatag ccacttcaga ttgaatctga tcctcagtgc aaaaaatatg
     6541 agcatcatcc tgcaccatat tgcgtacccg catcaaccca tgcagcgcgc ctgatggctc
     6601 acagcgatga caatttccaa actcagatag tctgagagga agatcccggt aacttttcaa
     6661 accatgatta taaatttgaa cgtggcaggg acagctcata ggttttactg cataatgtcg
     6721 attctctgtt tcggtaacaa acatttcatc tctaaagttc gcccaatgac ctgatttctc
     6781 ccataacgat ctgtctacta attgcggtgt tctaatttcc tgatagccaa aatcgaccag
     6841 gcgatttcgc atgtaatgct ctagctcttg ataaatagtc catcctttgg ggtgccaaaa
     6901 aaccattccc ggagcaatat cctggaaatg aaacaaatcc agtgccttgc ctaatttacg
     6961 atgatcacgt ttttctgctt cttccaaacg aaacaaatat tcttccaggg attttttatc
     7021 agcccaggca gtgccgtaaa ttcgctgcaa catttcgtta tttgagtcac cacgccaata
     7081 agcgccagct acttttgtca atttaaatgc ttttaaaaac ccagtggagg ggacatgtgg
     7141 acctcgacac aaatcttcaa aatcaccttg cctatataga gagagcgctt cattttcggg
     7201 tatatcagca ataatttttg ccttatattc ttcgcctaaa tccttaaaat attgtattgc
     7261 ttcatttcta ggtaattctc gacgagtgat cgaaagatta gcctttgcca gctcatgcat
     7321 cttcgcttcg attaaactca aatcatcggg agtaaacgag cgctcaaaag caaaatcata
     7381 ataaaacccg tcttctatta caggacctat agtcacttga gcggatggaa ataaggcttt
     7441 tacagcctga gctaataaat gagcggtaga atgcctgatt atttcaagac tgtcttcatg
     7501 tttctcggtt acgataatca aagagcagtc ttcttttata agataggatg tatccaccaa
     7561 gacgccatcg acacggcctg ctatcgctgc tttagctaat ccagggctaa tgtgatgtgc
     7621 cacatcataa atagtgagtg gcgcttcaaa atgttttaca tttccatctg gtaatttaac
     7681 atttggcata atcttatccg tctttccatc tttcaggttc ttgttaaaaa aaaaccctgc
     7741 atggcagggt tcaaaccttt aataattcag tgcagtagtt taccaattta gggattctac
     7801 tagaaatcca ggaattatgg taggcacgag tgggatcgaa ccaccgacca ccaccatgtc
     7861 aaggtggtgc tctaccactg agctacgtgc ctataattca acctgaatga aaattggcat
     7921 gaattataca cataagattc atggaaatgc aatcatccaa atcatatcat ttttatctaa
     7981 tgaaaaagca agataacatg acattaaaat tttggatgag catggcctat caatcttgcc
     8041 ttttaactct tttgcaaatg caacccttct caactaaacc gcaaaacact cttgctttcc
     8101 ccaaaatgca ttagtgaaag aataaagagc acgaaaagct aattccaact ctcttgggaa
     8161 caaggtaaaa tgataaagcg tccgttagca tttaaaacat tatgtggttt aaaatcatac
     8221 attcccatgt agaaatagct atttatgcca aaccgaacgc caagaaatca aaactcatgg
     8281 ccatttctga tgacaggctg catattgcgc tccatgccaa accgcaagaa ggggaagcga
     8341 ataatgagct tttattcttt atatcccaat ttttcaaaat tccaaaaaca caaatcgagc
     8401 taattaaagg aaaaagcagc cgtcataagc taataagatt acccttatca gaatctgttt
     8461 tctgctttct aaataatcca aaaatctaga acatatcacc ctttaaaact tagaaatggc
     8521 ccgaatgaag gggttcattc aagccatgca atgcgaggtt aaccgctatg gttaaaccac
     8581 ttggactacc cggtgatact tgttccttca agtaacatca gcgtccgata tttggactta
     8641 aagaaacgtg ttataacgtt tcaataccca ttataatcat ttaacaccaa aaatcaacca
     8701 atgatttcca gtagaaacaa tttgtttcca tttaatggat atatttgcag gtttaactac
     8761 cctttgctac acttttgtca ctttttaata tcttaaaatt attactccaa tatggaaaaa
     8821 gttaacttga caaaacagtt tgcttaccgc ttgcgtgatg ccatgattgc agcagggcat
     8881 aattctcaac gctctccatc aggagtatgt attcataaat tagctgaaat gacaggttac
     8941 tctttgcaaa tatgcagaaa ataccttcgt ggagaggtca ttcccgatcc agtcaagctg
     9001 atagaaattg catcgaaact aaatgtttca ccaggatggc ttctgtttgg tgaaacccat
     9061 aatgatcctg gaacatctaa ggaacacatc acgataagca aaagtttact ccatcatatt
     9121 tttacaaaag cagcttgtct atataaccgt gagcttgtgg aagatgatgt gtctgacttt
     9181 ttgatggatt tgattaatga cattagtctc atcaatgcca ccgaagaaca gtccaaaaaa
     9241 ataattgatt tggctatctc ttcagtgagc cattttagac actcgcaaag aacgtagagt
     9301 aagaaacttt catggaaaat agcccaaaag agatagtgct ccactctcaa cttttagaga
     9361 actttttcgc ctttaaaagt aaagtatcca atgtctttag agacgtattg ggcatatatg
     9421 actttcattc atatttcctt aatgcgtatt acaggacaca atcaattgct gcccctatcc
     9481 tctactcctg ccatggaatg taacttattt aacagttttc tttagcaaca tgagctaacc
     9541 tataatccca agtgatttaa attatgtact caaggcgatt ggcacacact ttaataatgt
     9601 aaaacactat gatgagctat attatcttga ccaaattaaa catggttttc ccatcgcttt
     9661 ctcccttgct accaagataa atgaaaatta tgtgatttat tcaatagcaa gccacaaatc
     9721 ttatgccttt actcgtgagt tattttctac tcatcaagaa gatttttaaa aaacagggcc
     9781 atattgctcc aatttgctgc atccattttt tagttattat gaagattttc cttctcaata
     9841 tttaatttta aactcacact gaataataag aatccacccc gactttataa tgaattactt
     9901 gatttttaaa gagctcgata atctacgtac gtacctcaat tttttcgaag cattttgtgc
     9961 gcattttatg tctatctcta aaactcagtg cttcatagag agtcggtcat ttgcccagag
    10021 acgcctgatt aggaatagaa atattctggt ctacactcag cgaacctgat actgcttttg
    10081 gtttcccgag caagctcagt tttattaaac tctccttaat tttacaatcg agaatatctg
    10141 ttgccaattc aagtgacgtc ttagggattt tgtgttttaa agtgtattca tccgaatcca
    10201 gcactttata ccttgatata ttgcgaagaa ggctttcact tgctgtttta tccacattgg
    10261 tgtgtctatt ttcagtcaaa aactggaagg ccttgttaat ttgctcttcc tcacccactc
    10321 ttgagtattt tccacacaga gtatccaggt tatacacata agttaatggg ctttgaaaag
    10381 gatcatgccc atgaacatac gtgacaaaat agccattgta ttttcctggt cgagcattct
    10441 ctacttcttt gctttcattc cagcgattcc atactaaata aattaacggc gatgcgtttc
    10501 tctcttcatc agtcatattg gttggatcgt taatttcatt attctcaaat aacaaatgca
    10561 gcatgttgtt cttcatataa atttgcaatt gggcattaag ttggtctatg gtttcagcca
    10621 atgcttcttt ggtggaatca ttataagtga cacccaactg agacgctgcc attcgaatgc
    10681 tatcaaagcg tatgggagca tggctataca aggtaatacc atcctcgctt agagagtaat
    10741 caataatttt taaagttggt ttataagctc tctcaactaa acttctcaat tcctcctcag
    10801 taatgacact ttgctccagt aataattgga gaccccagaa tgatttaatt tgtatatccc
    10861 caatataacc tttgggttta aatggttgac cgactacaag tttttcataa gcatgaataa
    10921 actcgtaccc atgattggat aaaatgatct tgattgcaat ctgattttga taaaggaagt
    10981 ccagtattct taaagtgaaa taatcacaac tcccccgatc ggccacctca tcacccagca
    11041 atcgaataag cgttttaata tcattaattt ctatctgcgt catgaactta ttaaatcgct
    11101 caatacatga tggcagcctt gttttcaacc cacttaattt ttcttccagg ccagctttac
    11161 tcttttcagc catttgtaaa ttggcctcta ttggcttctt aagttgcaat aatgattgag
    11221 aaaactcttt ctgatggtct gtcgcaagtg ataatttttg ctcaatgtct aaaattcttt
    11281 gctgtgcgtt agttattttt atttgtatca attgaagaag cgtacgaatt tccaaatatt
    11341 cttgaaccat atcgtcatat tgttcataaa ttgttacaaa ttgctggtaa gcttcgtgga
    11401 aattgataat ttctgtttta aatttaatta ttttatgacg gaaaagaaaa tggataagct
    11461 tgattgcatt gccatgcaag tcgcccagtg aaatggaacc taaattatct tcgaattcat
    11521 taggataatt gtatatatcg atattcttgt taatgagacg ttttggcatt ccctagtcca
    11581 tgtaaaaaac tccccttcaa aataagggca tactcaaagt cgacaataac aaatattcac
    11641 ttttcatgta ataacatgga agtattaaat tcatacaatg aactcccact ttaattaccc
    11701 tggaatagca gcaaacaagc taaattattg aacaattttt atgggtttgg ggaaacatga
    11761 atcttcatat ctattcccat taaacaatca tctaaccaaa aaacctatct gctatactta
    11821 aacaaggaaa ataatagtca gagaaaggaa tgagattatt atcttttaaa aaatccaaaa
    11881 ttgtttcaat tggaactgaa ttggagtttc agattattga ttgcagttca ttgtctctgg
    11941 tttctcgttc aaaagaattg atgagagcct taaaagacat gcgctatcgc gaccaaatta
    12001 agccagaaat tacgcaaagc atgattgaaa tcaattcatc aattcatcaa tccgctaaag
    12061 aaatgtatga cgaactgtta gagctacaaa aaattttagt agaaactgct gcaagcattg
    12121 atattgcttt ttgcggcgga gggacacacc catttcaaca atggaccatg caaaaaattt
    12181 ttccatcgaa acgattcaaa aaaaaattta accaataccg ttatctcagc aagcgcgcta
    12241 ctgtttttgg acaacatatt catatcggct gcccaacagg agacgatgct atttatttaa
    12301 ctcatgcctt ggcaagatat gtccctcact ttattgcaat cagcgcttca tcgcctttct
    12361 atctgggtat taatacaaat tattgctcat cacgctcgac tatttttaat gcttttccat
    12421 taagtggtgt cattccctat ctgagaaatt ggcaagaatt ctctgattat tatcgcaaaa
    12481 tgtatcgttg gaaaatcatt gaaaatatga aagactttta ttgggacatt agaccaaaac
    12541 cagaacttgg tacaatagaa ataagagtct gcgacacccc tttaactctt agaaaatcga
    12601 ttcttattac tgcttatatt caagcattgg cactctatct cctcgaagaa aaatctgtcc
    12661 agctgtctca tgatttatac tatgtctata attacaaccg gttccaagcc agcagacatg
    12721 gtctggaagg agaattaact gtcactgata aagacagacc catacctatc atggatgaca
    12781 ttcttgaaac aataaaaaaa attgaacaat acattaatgg acttggaaac agcgaatata
    12841 ttgaggaact gtatagtgac gtcatcaata aacaaaatga tagcgttctc ataaataaaa
    12901 tttataaaca agatggctca tttagcaagc tggtagcagc acaatgcgaa ctatggttaa
    12961 gtgattcaaa ggataggaaa tggatgacac aaccttctta agtacgaaag aaaaaatcct
    13021 ggaaatcaaa ccattaatgg aaagtgcttt cggcaatatt ctagggtatc aagaactgga
    13081 gaatatgttg gcaatctgta cacttgtacc attttccaac aatcaaatct tgtttcatca
    13141 aggggataga ataaaaggca tttacatcat tataaaagga actgttgatc ttattgccag
    13201 aaccttaggc aaaggaataa ccaagataga aacttgtctt gccagtgatt ttttgggaga
    13261 aacctccttt ctcctcaatg agcccagtgc cacatcagcg gttgctaagg acaatgtgac
    13321 atgcttattt atcccttctg cctattttga actgatgccg gcattttcac ctgaggccaa
    13381 atacaaaatc atgaaagcaa ttggttatca ggcctgtggt cgtttaaaac aattacacga
    13441 caaaattatt gattttattt ctcattctga tatgcaaagc ttatcttttt tggggcgagt
    13501 cattcagact ttcacacaac ctaaaataca caccctggat gagagtggag ttgataaaga
    13561 ccaaatttat aaaaagactt tatttaactc ttttactcaa gatgaaaccg atgaattgct
    13621 tgaacacgcg ataccactaa ctgccaataa aaattgcgta ttgattcacg agaatgaaac
    13681 cataccggta tgttatatag tgatctatgg ggcggttcag tccaatgtca tacaaaacag
    13741 taaaattgcc aaattgtctg ttattggtcc tgactcgttg tttgccagta cggcctgttt
    13801 tctgagtgac actaaatttt caattacatt tattacctgc gaatcagcaa ctttattaaa
    13861 aatatctgaa gccgaacttc aatatttcca aaaaaataaa cccatgatct ggtacaaatt
    13921 atttaatttg atatgtggat ctatcatttc ccttgctcgt tccttaaaaa aattggacgt
    13981 gcgactgaac atagaaaaat ataatcagtg aggtactatg tgcagaatat tgtcttacct
    14041 tgggcaaccc acagtagttg aagagttact ttataaaccg gataactcct ttattaaaca
    14101 gagttatcat cccaaatatt tgccttattt attaaatctg gcgggttttg gtatggctgc
    14161 ttgggataga aattcaatta accccttaca agcttatgtc tataaaacat ccaaacttcc
    14221 tttttacgat gaaaatttac ataatttagc agaaaaaata gcacctcact gtcttctcgc
    14281 ccatctacga ggggtgtcct atcatgataa gcaagtagtt actgatcaaa acgtgcatcc
    14341 atttctctat cccgtaacca gtattgcttt agcacacaat ggtatgctcc ataactttga
    14401 aaccatgcgc tatgatctgc tgaaatatgt taaaacggaa tacagaaaat atattaaagg
    14461 aactacagat agtgagtgga tctatgctgt ttttctttct caattagaaa atcccaaggg
    14521 tcattttgat gcctctgaaa tcataaatgc tgtaattgcc actttaaaaa ttttaaaaac
    14581 ggtacgccaa caaaacaaca tagtcattaa ctcaccagtt aatctttttt taaccaatgg
    14641 tgattttatt gccgcgactc gattttgttt ggattacggc tggaaaccca aagacgctac
    14701 tcaatcgaca catttcacct atcattcact atggtacact tatggagaac gatatggatt
    14761 ttatgaaaat gaatataaaa tgaaaggggg aaccattaat tcaagcatta ttattgcgtc
    14821 tgagccatta actgaaaaca caacaacctg gcttgaggtc cccgaatata ctttgatttt
    14881 tgcgaatcaa gtcgatggtg aaatcaaaat tacttcccag gatattaaca tttgaaatgc
    14941 tccatattta gcatcccaag acgctttctc atcatctcct tgtgcctttc tcatcacttc
    15001 attccaaaat tttccggtaa tacagaaagc aagaaaggta attgaatgcc ccgctcaagt
    15061 gcctaaaaca aggtgtccaa cattctgttt tcataaggta gaaaatcggc attccacttg
    15121 atacgattca ttaaatcggg attagcaata aatggccttc ccaaagcaat taaatcgtat
    15181 tttttatttt gaatatcttt tcttgcctgg tgaagcgtat agctaccgct ggcaattaag
    15241 gttcctttaa aatgagctct cataaattct gtcatcgttt tgaaccctaa ttctttgaat
    15301 tgaataccgt catcaaaatt acctgtatgc acataagcta ttccatagtg attcaattct
    15361 tccaatacat atcgaaatac catggcatct cgctcatctc cgacaatttc attaagataa
    15421 gctccgggag aaaggcgcaa tcctacagat tgcccaccta tttcattgat acaggctttt
    15481 accacttcca gcgcaaatcg ccccatattc tctggatatt gtccatactg atcatgacgt
    15541 tgattggtat ggtaatgtaa aaactgatca atcaaataac cattggcacc atgaatctca
    15601 atgccatcaa acccggctat catcgctctt cttgcagcca acgcatagtt ttggattaac
    15661 tcattaattt cctctaatgt agccgccctt gattttccat aataaagatc atcggcacgc
    15721 cggaccttac ctgtcatcat cgtttctgaa gcagaaatcg gtaagttacc atgtaaaaaa
    15781 atcggatgag ataccctgcc aacatgccaa atttgcaaaa atattttccc tccccgttca
    15841 tgcacagcat cagttaccat tttccaaccg tcaatatggg tttgcgtaaa tattcctgga
    15901 gcattgctat aaccctgacc atcagggcta ataatagtgc cttctgtaat aatcaacccg
    15961 gcatcagccc ttcgagcata ataatctgcc atctgcctgg ttggactgaa atcatcgtta
    16021 gccatattac gagtcatagg agccataatg actctgtttt ttagtgtaat tttatcatta
    16081 agctgataag gacttaaaat taaatcggag ttaaccatga taaatctcct gcatgtcata
    16141 acaatacatt ggaatatcgc aaatattcga tttattcaag caaaattttt taaatcataa
    16201 ctttcaattc atttataaat tcattaatta ggcttgcatt cgccttacaa taggtccact
    16261 gtcctctacg ctcagtgaat atcaaaccag cttgctgtaa ttgtgataaa tattgagata
    16321 ccgtggattg agataagcct gatttttttt ctattaagcc aacacaaaca ccatctttgc
    16381 tcacatcaca gtgcgacgaa ctgaaatgtt tatctggctc ttttagccat ctgattatct
    16441 gtagtcgctt ttcattagaa agcgctttta atatatttat tacgttcata ttttaattat
    16501 atcgttttat tgcgatatgt caatttagtg attacccatg gtcatgtgtg ttgaaatttc
    16561 aaataacctg cccgatacca acactaaaaa taagacgaat gagagagata aatgctcaat
    16621 atgggtattt tgaatattac ccctgaaaca gttcgtgcga caaatagtat tgcagaagag
    16681 cttgttgatt taactatttt acactctcaa aatttatttt aagagccttt aaaatctatt
    16741 ttcattcagg caatttatca aaacttttca tgacggattc attaaactct ttcattacat
    16801 cactttcata aaaatctttt ttaaattctt catcctgaat atcaaccatt ttaaccaatg
    16861 cgtccaagct cttgattgct tcatttattt cttcagataa ttttgataat ttttgaaaat
    16921 aaacttcttt atcatcccca ttataagatg aaggatcatt tattttcata tcgacttcgc
    16981 ttttcatttc cctgagcttt ctatccaatt gaatgatact ctggggcgta tttgcatcaa
    17041 aatgagttaa ttcttcttgc gattgattat cgatcgtatt attcatcaat ttgctcctgc
    17101 caaaataatt aaaaccttac tattaaattc tagtcgaagt acagcatttc ttcatttgta
    17161 ctattagtgc tatcattgtc tttttaaata gcagcaaatc atatgttact tcattatctt
    17221 tttattgtgg ggatttgtgt agaggcgatt acaggagccc tggcagcagg acgtaaaaaa
    17281 atggattttt ttggggtgct acttattgcg tcaatcactg cactgggagg agggactgta
    17341 agggatacat tatttaacac ctatccactg acctgggtag ctcacccgga atatctttta
    17401 tatacctcag tatgcgcttt tctaactatt tttattgctc attcactggt tagaataatg
    17461 aaagtattct taattcttga tgcccttggt ctctcaactt ttgttattat tggtacacaa
    17521 aaagttctcg cccatggtat gccaccaagt gttgcagtaa tcagtggaat gattaccggg
    17581 atttgcgggg gaatgttgcg agatatttta tgtaatgaca ttcctctggt tttaagaaaa
    17641 gaattatatg ctgttattgc cctggctggt gcgcttctgt ttataacatt agactatttt
    17701 catatccctc ataatttaaa tatcattatc actttgttat gcatttttac cgcacgctta
    17761 ttggctattt tttttcatat tgaaattcca atttttgatt actcagagag tataaagagg
    17821 agaaaaaaat aacgtttttt ttcactaaaa cacttaaata atgatgttaa tctcttagag
    17881 ataccccatg atattttgcc tctgaaaaat ggcacgctgt tttgcaaatg cggcttcctg
    17941 catgtataag gaagatccct cttcctactg gggatgaaat taaagaaaat atacttttgc
    18001 catgaaaaac ctatgctatt tcagccatat attttctcgc taagccaatt ctctaacctc
    18061 aatgatgctt atttcttcta cagggacatc tgcatgacca tttcgttggc ctgttttcac
    18121 tttagcaata gcatcaacca catccatccc ttcaactact tctccaaata cgcagtaacc
    18181 gtaaccttga ggatggtcgc cactgtaatt cagaaaacca ttatctgcaa cattaataaa
    18241 aaattgggct gttgcagaat ggggatccat tgtcctcgcc attgcgatgg ttccacgttt
    18301 attaggtttc gctccttttg cttcattttg aattggagag gcagttgttt tttgctccat
    18361 atttgtattc aaaccgccgc cttgaatcat aaaaccatcg ataacacgat gaaaaatcgt
    18421 gtcattataa aaacctttac gcacataatt taggaagttt tctgcagtta atggggtatt
    18481 ttctgtatct aatttgatat gaatatcacc catggatgta gaaattaaaa ccattatttc
    18541 acctcttaat attaaataat ttaggaattt attatcaaat gacgcatgtg attacaaaca
    18601 tcatgcaaaa catgaacatt atggatgctt gccaaagcag taaataaatt agctttttgt
    18661 tttgataaat gatgcaaata ggatcttgaa taattaagac aagtataaca agaacaactc
    18721 tccataacag gtgccaaatc atcagcaaaa catgatttct ttatttgaat tcgtccattt
    18781 tcatgttcgc tttgaaattt tagccaattc ccttctctta ccaatttacc acaatataag
    18841 gcaccatgtc gggcattacg tgttggtgca acacaatcaa acatatcgat accttccgcc
    18901 accacatcca acagatcttg aggtgacatt ccaactccca tagtataacg tggcttattg
    18961 cccggaagat actcatacac ccaacgaatg acttccactg tagtatccat atcaaagcct
    19021 acagtttctc cacctattgc aataccatcc gtattcattg cagaaacaaa atcagcactt
    19081 tctcgacgta aatcttgaaa aactcctcct tgcacaatgc caaataaagc ttgtttatat
    19141 ccgtatcgag agttgggata ttgttgatgg tagtcaatcg attgttttag ccatctgtgg
    19201 gtccgcaaca tgatttgact tacttcatcc ttggagcagc tgtcaggagt acactgatca
    19261 aatgccatta taatatcggc accaattatt ttctgggtct ccagagacat ttccggggtc
    19321 atatggataa tccccttact gccaggcaat ttaaaatgag caccagattc atctatagaa
    19381 caaatttcct tgttcttcga taaactaaaa acttgaaaac caccgctgtc tgttagcata
    19441 ggtccatgcc accccataaa gcgatgcatc ccccctgcat tttctatcac cttcatgcca
    19501 ggcgcacaca acatatgata ggtattaccg cccagaataa tttgagtacc cgcagcagac
    19561 agttctgacg gcatcatgtt attgacccct gcacgggttc caactggcat aaataccgga
    19621 gttataaatt caccatgagg cgtaaccaca cgtgtaacac gagctttggt acccgaatgc
    19681 tggtacagta cttcacatga aaaagtgttc attcgcagaa acggcaagat aaaacaaaga
    19741 atgataacga aagcacgcgt ttaatgccag gaattaattg atcgttcggg ttcgaaaaaa
    19801 cagaaaaagc cccattatcg ccataaacag aaaatacatg gcagcccacc caggcataga
    19861 aattccaaac atagtccagg tcacttcagc acaatcccct gctccccaaa atagggcttt
    19921 agcaacagtt tgccatggaa aatactgaat taatacatca agcccaggca tgcaagcagg
    19981 agcttgatct gacggtaaag attgcaacca caattgcctt aaagagaaat acaaaccggc
    20041 acacgcaacc agaaactgca tcaagcttac tatatgcgct tttttcaagg ttcccagaga
    20101 caaccccatt aaccctaaca aaataaatac acaaattcgc tgcatcaagc acaaaggaca
    20161 tggttgtagt ccaacagcat attcaaggta aaacgaagcg aatattacca ataaagtaat
    20221 aattacctga aaagattgta tttttctgta agtaagcttt ttcatgattt cggcaacata
    20281 tattgtatcg cggcttttaa ttcagcatca gaacagtcca tacaagtacc ttttgctggc
    20341 atggcattca accccttgat tgcagaagcg agtaaatcat ctattttctt acttgccaaa
    20401 cgaggtttcc aatcggcctc cgattgaaac ttgggcgccc ctgccaatcc atctctatga
    20461 catacggagc aatatttatc atatatatct tggcctggtt cttttttgct cttggaatca
    20521 acaacagtcg ttttagtcgc tccagcttct atgttattcc cctgctcctc ttgaactcga
    20581 actttcccaa ccggttgaat tctttgtaaa atttgttgct catccacctc gtccattgca
    20641 aaaagcatga ttgaaaaaaa agacaagatc agcgcaatat tcaacctcat atttatccgt
    20701 cctctaatag ttaatttaaa aatcataccg acaagtgact tttttttcca gaaagatttc
    20761 aataggccca atgatttata aaatcattag ctgaatactt agacttttac tgaaaagatc
    20821 cttccactat cagccattgt gctaaactgc acacaaggga aaagaatctg agttatggat
    20881 gagcaataca gaataccaaa caattgataa caagactcaa tatatcaatg gaaaagaaaa
    20941 acatcaaaac catgcttatc ctgagtacat caatcattcc atggaaaaat attacgaggg
    21001 acgagtcaat aaatcctggc aagggaaaca catacttaaa ggcaaaaccc cgactgcaaa
    21061 ttcactgatt ttttcgagca atgattattt aaacatcagt caacatccgc aattaattaa
    21121 agcgcaaatt gctgcgatgc aaaaatatgg taatggtcaa atgcaatctg ctgtattttt
    21181 aaataacagc tcttttttgc tcagccaatg tgaaaagaaa ttcagttcct ttttaaatta
    21241 ttctgcggcc ctgctcactc aatcaggctg gagcgctaac gttggtctca tacaaaccct
    21301 ggccaagtac aatacaccag tgtatctgga tttttatgcc catatgtctt tttggtctgg
    21361 tgtaaaagca gcaagagcca aatctatacc gtttctacat aattcagtcg attcactaaa
    21421 aaaacgaatt gagcaccatg ggccaggaat aattgcagta gattctattt atagtacgct
    21481 gggtacgatt agtccattgg ctgactatgc tgacattgca agaaaattca aatgtttgct
    21541 catcgtcgat gaatctcatt cattaggtac gcatggttcg caaggcaagg ggatggtggc
    21601 taaactggga ttatcaagcc aaatagacat cataacagca agcctggcta aagccttatc
    21661 aggacgggga ggcatcatta caggagatag gcaacttata gaatatatca gatattcatc
    21721 tctaccctct atttttagtt ctgctttaat gcctcatgat ttagcaggat ttagcgcatc
    21781 attagaaatt attactcaag aagaatggag aagagcaaaa ttacaaacca atgctgattt
    21841 tttacgagaa gccttatcat gcgaaggaat taatatagga agtagtggct ctcaaatagt
    21901 ccctttagtt acaggcagcg aggctaacac gctttggtta agggatgagc tagaaaaaga
    21961 agacatcttt ggagccgtgt tctgttcacc cgccactccc aaaaataaat gcctgattcg
    22021 tttatccatc agcgcgcatc acgaaaaatc ggacttgctc agagtgattg agtgcctggt
    22081 cagacttgca agaaaaagac ccgatattcc tttgtttaag ggtaattaac acgacttcta
    22141 attattggcg gctcatttga acagttaaca tttcaaataa gaattatgtg ctctttatat
    22201 agaattccta ttaattcaga aacatttcct ctgcatctaa ctcttccagg tattggtgat
    22261 aagaaagaat tttatccagg cgcacatcga acaaaacagc cccttgcaca taagcataaa
    22321 aataggtttc atcagaaaca aatcggttgg caggaaccaa acaattcgac cattcattcc
    22381 attgaggtgt cactaaatta gattgccaaa aacaaggaat tttttctcca ttatgcaatt
    22441 ggtccaatac atctccacta gctccattat ccaaagccaa atcatagtgt aactgcatat
    22501 ccgcttcgtt tttcacgatt aaaaaactga tgttggcatc atcatccagc atcaaaaagc
    22561 tacctgagtt atcggccaga taaaattcaa cgattccctt ttccctgcat aaacgccaga
    22621 ataactcagc atattttttg tcatgtaaac aatttggcga tgtcaccgaa agcatgcgta
    22681 caatcatatc agacatgctt tgaaaatatt gtaactgcag gtcatggatg ctttgagtaa
    22741 tgagactggc gacatccgga tcactctttt tgatgtatct atgaattaat ccctcattaa
    22801 aagcagcgat tgccagcttc tcatctgctt gcccagttaa taatattttt ttaatattag
    22861 tatcttcgat tcgcctgcaa aactctaatc catccatacc cggcattgca taatcaacaa
    22921 cgactactga aatttcagaa aatcgtcgag agttataaac ctcggcatga atagcagcca
    22981 aatcaagatt aatggtatga ttagttagtg ggcaattttt tgcttctgtg tattcactga
    23041 gacaacgttc acttaataat tccagttcac agcgtttttg tttaatacaa tctaaagctt
    23101 caaaagggga atcaaataca cggtacgcca aaccctcatc cagctgcaat acgaaattga
    23161 gcaagaaatc ccgactgtcg tcgacaaaaa ccgcagtgct tggaaagtaa caggtaggta
    23221 ttgagaaatg ttgcatggct cctcctgagc aaaacgttcc aaagttatat cctttctatg
    23281 aacttcaaat aacctgtggc tccatcatca gaacctggct aacttgtgaa taaagtaaca
    23341 tgattgttta t
//
LOCUS       DACTJE010000035        23149 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.035, whole genome shotgun sequence.
ACCESSION   DACTJE010000035 DACTJE010000000
VERSION     DACTJE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 23149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23149
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.035"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            69..2285
                     /locus_tag="I9760_001061"
     CDS             69..2285
                     /locus_tag="I9760_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042837.1"
                     /translation="MIRTFLTAFLMLLCSLALAQEKVVTQFFKEVNSSIAISEGKANI
                     QASRYRVVDIDVNQLYAELENAPHRDGISTGIPLQLELPQPDGTVKRYQVMENSTLAP
                     ELSTKFPEIKTYDAYGIDNPGELVKFDLTPQGFHAMILSPGRDTVFIDPLIKGNTQYY
                     MIYYKKDFITSKKMKCGVKNQNQPLINAASKRDFTDFNPCVLKKYRLALAATAQYTQF
                     HGGTVPQALAAEATTVNRVNGIYEIDMAITMQIIANNNLIIYTDPNNQPYTSGDPDKM
                     IGENQANIDKEIGAANYDIGHVVDAAGSGLAQTPSVCINGEKAMGVTGQSNPVGDPFD
                     VGYVAHEIGHQFGANHVQNNNCQRNNPTAVEPGSGSTIMSYAGICDPNVQDDSDPYFN
                     GISLQEMGNFVSDATHTCPVKTSIPSAPVIQGTNGGVKIPAQTPFALTATATKSGGNE
                     ALTFAWEQQNNEASQQPPVSTSRVGPNFRSFSPQVSGTRYFPNLNALANNGPFTWEVL
                     PSVSRTLKFRVTVRRNTPGGSCNAYTDTTLSVESKAGPFVVTNPTESGITWTGISPRT
                     VTWDVANTNLPPINARFVNILLSIDGGQTFPFTLLSNVDNTGSEVICVPNLNSSTARI
                     MVQASNGTFFNVSKNNLTIIPVPPRPPELTRADRNPMDTSKAFILYANCIPTGNDVYT
                     VNGLPPGATVRFDANNRRFIIENINTPKRVRNVTITATDENGVSRTSNAITIPSIL"
     gene            2298..2999
                     /locus_tag="I9760_001062"
     CDS             2298..2999
                     /locus_tag="I9760_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HAT5042838.1"
                     /translation="MNRYSIFFLIFCTLSLESHASLYSGINLGINAVKINKNLVYPLE
                     DPVPTTSSFNSAYTDFHGQLIAGYDFSLAPKISAAVEANIDLFTGKAKHRITEWYFNE
                     DVYAEEQLKVGYSLFLLPTYHLNNMVQFFVGPGVSSSRFNIAFSNTAGNVGVSDSLNE
                     WLTGISIKAGSITKLTRNIDLLLTYQFTQYNSVTGVQMEPLSEDTLRGSYKPNVNTVL
                     IGFKVHPSDEITKDT"
     gene            complement(3068..3259)
                     /locus_tag="I9760_001063"
     CDS             complement(3068..3259)
                     /locus_tag="I9760_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042839.1"
                     /translation="MECKCNQCGMGVKNLTCSKCDALLVSGTIVKNDGNKVQVAKCPN
                     GCGQIKSPVCCGHDMACSV"
     gene            complement(3314..4174)
                     /locus_tag="I9760_001064"
     CDS             complement(3314..4174)
                     /locus_tag="I9760_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042840.1"
                     /translation="MVSYLFSEVHFTHALKKVLGISVLVGVYSAIIYFWFEQDNLHSS
                     NWLSYSQSVFGIILGLLLVFRSNRAYERWWEARTLWGQLVNASRNLAIKIRILLSPNA
                     DEAKKFSELINTFCEVLAIHLRQKSTRDDFKKFLKMVSLPNHVPSYVAQELYRELSEK
                     TKASKRLNLWLLDNEFSKFMDICGGCERIKNTFISLSFRVFVKHVFIIFILFLPCSLI
                     DTLGVWAIPATIIITYLIIAIEGIARNLEEPFGLTEDHLNLSAITESIRESVNEVLLG
                     QKSNEESNSI"
     gene            complement(4351..4587)
                     /locus_tag="I9760_001065"
     CDS             complement(4351..4587)
                     /locus_tag="I9760_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042841.1"
                     /translation="MKRVHHHPATHSHPSNATVQVSQGQTTVVVNPNPPHFPGQLPMY
                     HPNSFFTTHHHSPYYASQNQHYHQQPAPNTHQHP"
     gene            4903..5778
                     /locus_tag="I9760_001066"
     CDS             4903..5778
                     /locus_tag="I9760_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HAT5042842.1"
                     /translation="MDSLDLDFYAITRAFLFFSLIGLMVVLEYFFSFHRLGKAGVRRR
                     RVSNWYLGIVNIIVALPLSTLTTAAYCEYYQIGLFNLLKVPFGLVFILWFIIYDLYVY
                     LFHRLSHATFLWHFHKIHHSDKSLNVTTSFRFHPVEYILFNALKIVLIILLGPYFFVI
                     LVSDFIQAILVFWGHSNIALNAKLEQALSRIIITPRYHVMHHTQDECRRNYAAGLTLW
                     DYLFHTQTEPLWNKDDINKLTLGVQTKTNWSEFKNLLLINKLIHFLGRLQFVYFKFPL
                     ITIGVAVIFGIVEFY"
     gene            complement(5914..6126)
                     /locus_tag="I9760_001067"
     CDS             complement(5914..6126)
                     /locus_tag="I9760_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0045 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042843.1"
                     /translation="MKSTNNMQKKETDKPTSEQEQKKEVRRNNAPDFIPGASPYYNLY
                     CFFYKLCHGEDTSRQHQPATSFQHGK"
     gene            6336..6734
                     /locus_tag="I9760_001068"
     CDS             6336..6734
                     /locus_tag="I9760_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042844.1"
                     /translation="MNCFPFFAKKNKKNNEEKAEKIFSVFPAGTSINKTTVKEICFTD
                     IFCDTHPFVWYYYPEHNLAIWTSRLAGTTQEYPADYLLNQLAKIPGITIDQGDAAIKD
                     VSTFLQGHQMIPIRNNESWRYIKSSSFSMS"
     gene            6933..7595
                     /locus_tag="I9760_001069"
     CDS             6933..7595
                     /locus_tag="I9760_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAT5042845.1"
                     /translation="MKPTDKKHWSKIEYLHQSVKNPNIHIKGKHSYYSDAWTGSFEET
                     VVRYLYGDEYSLQTWQPQWQIDQLYIGDYVCIAAEVIILLGGNHNHRADWFCLYPFAD
                     KYVEAYQGKGDTIIKDGVWLGMRAVIMPGVTIGEGAIVAASSIVTKDVEPYSIVAGNP
                     AKPVKKRFAENVIERILALGIYSWSEAKFNLLKDYICSNNIDKLEQASRSYDQNHTIE
                     SK"
     gene            7592..8470
                     /locus_tag="I9760_001070"
     CDS             7592..8470
                     /locus_tag="I9760_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5042846.1"
                     /translation="MINIRKLRLGDESTLETFLKSHADESMFLRSNLYHSGLSYHDKP
                     FHGDYFAAFDTNKKISGVLAHYWNGNVIVQCPDFNVLTALVGTFQKAATREIAGILGE
                     DQQADVVMKQLQLEHVDYAVNYPDGLYALNLQNLAMPGNQDRYQLKVAKDCDKCLLYE
                     WLRAYQIEALGAEEDSSILKEQVESEVKSTLEGNDRWVLEENGTPVSLCSFNARLSDI
                     VQVGPVWTPVTLRGRGYARTVVALSLSAAQKSGVSRAVLFTNNQAAVRAYQAIGFNKV
                     GNYRLAILKKPILLTR"
     gene            8523..9818
                     /locus_tag="I9760_001071"
     CDS             8523..9818
                     /locus_tag="I9760_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT5042847.1"
                     /translation="MDMDIKHKPLKRELSLFGATMMGLGSIVGTGVFVSIGIAAGVTG
                     PSVILAIALAALVATCNAFSSAQLAANHAVSGGTYEYGYRYLHPAFGFTAGWMFLCAK
                     SASAATAALGFAGYFIHLSGLETVPVIPIALAVSITLILLVLSGLRRTNTVNIIIVMI
                     TLITLTVFIISGLPDFMQRGTANLRPFFPVSKQEGLSHFFYATALMFVAYTGYGRIAT
                     LAEEVKNPGKFIPAAIIITLMVSAVLYILVGLVAIGAVGVEKLAQVTETNATPLESAA
                     RAIGIPGLSVLVSIGACTAMLGVLLNLILGLSRVALAMGRKGDLPKMFCYVSETRHVP
                     VSAVIGVGVVIIALVLTGSVETTWTFSAFTVLIYYAITNLAALHLPKEEQLYPPLFAI
                     GGLLSCLFLAFWVPIGVWITGISLLFLGLLWKVVANRFL"
     gene            9882..10748
                     /locus_tag="I9760_001072"
     CDS             9882..10748
                     /locus_tag="I9760_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HAT5042848.1"
                     /translation="MIDGIMVLWFILLMLSLAFIAYDVREMPIDWVQKLGWVLVVAYT
                     GPLGLFFYFLTCRSPGKGLHALYTKAQWKQAVNSEVHCLAGDATGIIFAAIFLSFIDL
                     SNGVELILEYISAFFFGWVIFQAGMMRSMYSSYGEALRKTFFAETISMNFVMMGMIPV
                     MMILMNTLDYGRNPAHLQFWFIMGMATVVGGFLAFPINNWLVKKKLKHGCMTLPEGDE
                     QIEGHMTHHHGGHEEHLQAQAKRIGSKVIHGHTHEMGSLPLNIQLKYIGISFGVLLII
                     VLIMAFFVPLKI"
     gene            complement(11236..11718)
                     /locus_tag="I9760_001073"
     CDS             complement(11236..11718)
                     /locus_tag="I9760_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAT5042849.1"
                     /translation="MWLDYLHNINSDPNLLYLFIRTFIIYVFAIFIIRVGNTRFNFET
                     AFDYIFVFVIGAVLSRAINGNSTLISAMAGSSLLIFLHWIFALCSFYSHGFGKLVKGK
                     SVLLIQDGQLIWSALKRHQVTEEDLRQVFREKLNASDLTTVKEARLERTGRISFITYK
                     "
     gene            complement(11931..12824)
                     /gene="prpB"
                     /locus_tag="I9760_001074"
     CDS             complement(11931..12824)
                     /gene="prpB"
                     /locus_tag="I9760_001074"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HAT5042850.1"
                     /translation="MSNFRYSQGKKLREALMNEKPLQVVGTINAYTALQAKRAGFHAI
                     YLSGAGVANASYGLPDLGITTLNDVLEDVRRIMSAVDLPLLVDIDTGWGGAFSIARTI
                     KEMIKAEAAAVHIEDQVQAKRCGHRPGKALVEKEEMIDRIKAAVDAKTDPDFVIMART
                     DALANEGLNKALERISAYIEAGADMIFFEGVRKLEEYQALTEQCNVPVLANITEFGVT
                     PLFTLEELEEVGVSLALYPLSAFRAMSAAAEKVYDTIRKNGSQNDILAEMQTREELYQ
                     VLNYHFYEDKLNELFMKEKTT"
     gene            complement(12950..13684)
                     /locus_tag="I9760_001075"
     CDS             complement(12950..13684)
                     /locus_tag="I9760_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAT5042851.1"
                     /translation="MLNKLMWLTGKAQEDWIEKILHLRQKIWPDYQRIKNDTLFKLYS
                     HYPLYQWLSHYNGEVVAVINSLPVALDQSLDNLPQEGLNWVLSHLESTTPLTQSNYLA
                     AVSVTVNPAYQRLGLSKLLLNRLKASAQEAQYKALIVPARPSQKENYPLISLEDYLKW
                     KRVDNTIFDPWLRVHAQLGGKIAYPCQRSRVITANVAQWEQWLGHPLLQTGQYLVKGG
                     LNPITINKEKDIGIYIEPNVWVIHDL"
     gene            complement(13677..14378)
                     /locus_tag="I9760_001076"
     CDS             complement(13677..14378)
                     /locus_tag="I9760_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3865 domain-containing protein"
                     /protein_id="HAT5042852.1"
                     /translation="MIREYMDKRNNQFTTEILNYFSDLKKFSTSYLEWITMEHYQFSL
                     NNTRFLMNSHIQTKSLSDPGVSEELLANYNEEKNHAQMYRHALKKIGLDVTNRIPYVA
                     TRQFFMRLEALIVINASKTLGVMYATETAAIFEHILFKEISAELCQRKGLDFENSRIK
                     QFHDLHLDGVEQAHKDELGKFMDHTLAEEHVDPNIFLQEKEIMDGAYAAIDIMEDWWS
                     GLINAKAHLLEQAHA"
     gene            14482..15390
                     /locus_tag="I9760_001077"
     CDS             14482..15390
                     /locus_tag="I9760_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT5042853.1"
                     /translation="MINFSQIQVFITIVESGGFIAAGEKMHRSQPAITNAIKKLESSL
                     GFELFDRKVYRPQLTAKGKAFYQKAREVVAQFMQLENSAETLRSGVETQFSIDLDIAL
                     PLPGYAPLIQSFIRQYPETCFSLSNNTFVRCLERLERRECHLCIAASHIRSPQIEYLR
                     MPSIRMIPVASRDYYDQHKERINNPKLGIYCMQIMIAGTQQELYQLKIEPLSHSMPKW
                     LVGDMYIRKQLILSGMGIGRLPDHLVEKELAEGSLVQLNTRDYVAVTMDIYAMRLNNQ
                     EHGMVSNYAWQSLKSYAEDTVSPDLK"
     gene            15535..16254
                     /locus_tag="I9760_001078"
     CDS             15535..16254
                     /locus_tag="I9760_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase-C39 like family protein"
                     /protein_id="HAT5042854.1"
                     /translation="MIDFKINSQPDDESCGPTCLHAIYQYYGLDISYEEVANQVESFL
                     SGGTIAPMLGKHALQLGFKVTLYVNNLQLYDPTWFNSDGSSNTDVLREMLMQQMRYKR
                     NQYLARGTHAALDFLTLGGAIYFRCLTAEILKEYFARQIPILTGLSSTYLYNCSRELF
                     NNEGKSRSDPIRGLPCGHFVVLCGYNQKHRLVVVADPYKKNPVSGDNYYTVSIHRLIN
                     AIMLGVVTRDGNLLIIEPKNS"
     gene            complement(16527..16931)
                     /locus_tag="I9760_001079"
     CDS             complement(16527..16931)
                     /locus_tag="I9760_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT5042855.1"
                     /translation="MNNKKIGEFAKLCGVGIETVRYYQRQGLLHVPLLNKELGAEKIR
                     RYGDDDISRLKFILLAKKAGFTLKEIKELLEFDAKNDRERVRDMAERRIEKLNLKINE
                     LTEARNRLSLLVTKCIETSSSPCPILMTFEKS"
     gene            17001..17744
                     /locus_tag="I9760_001080"
     CDS             17001..17744
                     /locus_tag="I9760_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HAT5042856.1"
                     /translation="MNHTSIKKAVLYRMVIEHHVCPYGLKARDLLRRQGYEVEDNWLT
                     TREQVEAFKDKHNVKTTPQTFINDERIGGYDDLRRYFGKKVRDPKEKSYRPVIVLFAI
                     TALMAMAVSYTRFANPFTPHALSWFIAFSMAVLALLKLQNIESFSTMFLNYDLLAKRW
                     VPYSYIYPFAEALAGILMIAGALNWLSIPIALFIGTVGALSVFKAVYIDKRELKCACV
                     GGDSNVPLGFISLTENLMMIGMALFMLLM"
     gene            17944..19035
                     /locus_tag="I9760_001081"
     CDS             17944..19035
                     /locus_tag="I9760_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042857.1"
                     /translation="MSKEIKKNLQNNNSAFVYLRSTGLATNSLSSILQYMTRIIVLTE
                     SAGIALGILGVLFSVIGFVSSTIARILDPTASKLSRVMSAGILIAAAAGAIPLIYLGT
                     LFPAMWVYLGIGIMGFGILNSSYYLYSDVRKESALINQYKDNPEQYVESIKEKRQAKY
                     EKLLNTRLDDKQARVLLVFKLYQLDKKLAFAKQEGPARLYDIVCHRKHTRSLERFIDL
                     GTSLTSLIVGVSGLFWGIAAWPIGPILLGISLAVFSVSLSKSAIQFYRKVNKNNALLK
                     EVLSNDRYVSLIDANLDNPYDIEEMGTVEKTQEKVCTHELSQESALSKDQEHKEHYTK
                     LFQPVNKTALRPNDEETDELSCKPVLLQR"
     gene            19155..19436
                     /locus_tag="I9760_001082"
     CDS             19155..19436
                     /locus_tag="I9760_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAT5042858.1"
                     /translation="MANVTKLSGIHFTDHDLRRTLITIAEGLDTSAYALKRLMNHKMN
                     GDITAGYIVTDVDRLRKPMPQITDYFLKCMGVQPSATLVAIRPQGAVHE"
     gene            19660..19842
                     /locus_tag="I9760_001083"
     CDS             19660..19842
                     /locus_tag="I9760_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042859.1"
                     /translation="MSILIKFESLAKQAIFSSNKDIILQSIGLLDMIKAKNNQKIRMT
                     LSNSSEQFADMSRVVR"
     gene            19857..20621
                     /locus_tag="I9760_001084"
     CDS             19857..20621
                     /locus_tag="I9760_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HAT5042860.1"
                     /translation="MKKLALVGSGIKSISHFTFEFNTYTTSADKVLYLVNEPITKQWI
                     ERYSKLSESLDPIYFAENDRQISYDKIRDKILTELETHNFITVVLYGHPTVFADPGLQ
                     SIIIAQKKSIETIVLPGISVENCLYADLKIDPGQFGCFHVEATELLLYDKIIDPTVHL
                     CIWQPGMIGNRSVPKPNQKSNHLELLRIKLKKYYPDDHISILYEASMYPGVEPIIHKF
                     PLYDIEDQNIGTLSTLYIPPLPQRKPNLDILKQITS"
     gene            20651..21829
                     /locus_tag="I9760_001085"
     CDS             20651..21829
                     /locus_tag="I9760_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042861.1"
                     /translation="MLSVLSYRLSLLPKLSKTCYLLLLFSFLECVAGGIAYYLSLYLG
                     TSTPLTKLQIGQVGSIVGFGALAGALCSTYITDKLNNKNIIIASFLFLGLGFFCLPQS
                     MSFLLISGCVFIIGFATSIFLTTNNTLLLQNAPQDQNSLRFIQSMKIVSENLGNSFSI
                     LLIMFFAAQYYGQIFEVIGLFFFLFALNTMKYLNVKQNSLEDSSYEEKETNQQGNNKR
                     LYVTISAVFIVGIIYAQQRIAYPLFLHEAFSSFTITAILFWLDPIIVSLFQVKITKIV
                     SPLKISHQLAIGGLLLGTGLFALLIVDSIGGAIMACLTFILGEMLFMPTSFVRCYESA
                     GNKRKGMVAGAWRSAYSSGLIVGPVLSGFLMEYYSYNACWILAGILGFFLFILFSVKK
                     "
     gene            complement(21830..23101)
                     /locus_tag="I9760_001086"
     CDS             complement(21830..23101)
                     /locus_tag="I9760_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042862.1"
                     /translation="MKTINPVFFELVDKLSPDNKFAMYLVDYGYGDLVGDDDGIFLPT
                     KTGQYVRLDSEYTPPSILNDLAYGLTNSPLGVFFNKSFEWFLNTSDEQINRTFPIYID
                     PPGTFFSIGHIINYGYTATHLPNGVLFVTAGSKSNFMIPKIGCGIMHSRIQKNHNITK
                     SAPRSYHEHADIFQDIVKGNRDNNGWSASVLYFSENWIEKISKDSAWQEVRDYFYRIS
                     NHRSEYAVNSDYYNHVYRVTNKRNNLKSNLLIYETARHLFEILLGAKLGFAPAMDDEL
                     LPLSIIQEVYSECYKLEYLPIIAIPAYYNYRDPKPVYYSLQYPSLCSYSPKARNASTT
                     LSDLIALIDVVHKYQKDFTLPIRECKNTILEEVSKITSFNFYHPATEENSIIKSPEII
                     PYQDERFRGEKRFPINASFFKGCVSIFSNSI"
BASE COUNT         7154 a         4162 c         4406 g         7427 t
ORIGIN      
        1 tttttcaatt ttaattggat ttactgttct aataataatt gattttactc aatttgatgg
       61 gttttacaat gataaggacg tttcttacag cattcctcat gctgctttgt tcacttgctt
      121 tagcacaaga gaaggttgtc actcaatttt ttaaggaagt taattcttcc atagcaattt
      181 cagaaggaaa agcgaatatc caggcaagcc gatatcgtgt tgttgatatc gatgttaacc
      241 agctttacgc agaattggaa aatgcgcctc atcgcgatgg tataagcaca ggcatacccc
      301 ttcaacttga acttcctcag ccggatggaa ctgtaaaacg ttatcaagtg atggaaaaca
      361 gcactttagc tcccgaatta agtacaaaat ttccagaaat taaaacatat gatgcctatg
      421 ggattgataa tccaggggaa ttagtaaagt ttgatctgac acctcaaggt tttcatgcca
      481 tgatcttaag tccaggtaga gatacagtct ttattgatcc tttaataaag ggtaatacgc
      541 agtactacat gatttattac aaaaaagatt tcattacttc caaaaaaatg aagtgcggtg
      601 tgaaaaatca aaatcaacct cttataaatg cggcttcaaa aagagatttc actgatttta
      661 atccctgtgt attaaaaaaa tatcgtcttg cattggcggc aacggcacaa tatacccaat
      721 ttcatggggg tacagtacct caagcacttg ctgcggaggc aactactgtc aacagggtca
      781 acggtatcta tgaaatcgat atggcaatca ccatgcaaat tattgctaat aacaatttga
      841 ttatttacac agacccgaat aaccagccct ataccagcgg cgatcctgat aaaatgattg
      901 gtgaaaatca ggcaaatatc gataaagaga ttggagcagc taattacgat ataggtcatg
      961 tggttgacgc cgctggtagt ggattggctc aaactccaag cgtgtgtata aacggtgaaa
     1021 aagcaatggg tgtaactggg caaagtaatc ctgttggaga cccatttgat gtcggttatg
     1081 ttgcccatga aataggacat caatttggtg cgaatcatgt acaaaataat aattgtcaaa
     1141 gaaataaccc tactgctgta gagcctggaa gcggcagtac gattatgagc tatgcgggaa
     1201 tttgcgaccc taatgtccaa gatgattctg atccctattt taatggtatt agtttgcaag
     1261 aaatggggaa ttttgtttct gatgcaacac atacttgtcc tgtaaagaca tcaataccct
     1321 ctgctccggt aattcaagga acgaatggtg gggttaaaat accagcacaa acgccttttg
     1381 ctctaactgc tacagcaact aaaagtggag gtaatgaggc attaactttt gcatgggagc
     1441 aacagaataa tgaagcatca cagcagcctc ctgtcagcac ctcaagagtt ggaccaaatt
     1501 ttagaagttt ctctcctcaa gtatcgggga cacgttattt tcccaattta aatgctttgg
     1561 ctaataatgg cccctttacc tgggaagttt taccttcagt ctcccgcacc ttgaagttca
     1621 gggtaaccgt acgcaggaat actcccggag gttcttgcaa tgcttataca gatactacct
     1681 tatctgtaga aagtaaggct ggaccttttg tagtgacaaa tcccaccgag tcaggtatta
     1741 cctggactgg tatctcacca aggacagtta cttgggacgt agccaatact aaccttcctc
     1801 caataaatgc ccgatttgta aacattctat tatcaataga tggagggcaa acttttcctt
     1861 ttaccctctt atcaaatgtc gacaatacag gaagtgaggt aatctgtgtt cctaatctga
     1921 attcgtcaac agcaagaatt atggtgcagg catcaaatgg gacttttttt aatgtttcca
     1981 aaaacaattt gacaattatt ccagttccac ctcgaccacc agagttaacg cgcgctgatc
     2041 gaaatcctat ggatacttct aaggctttta ttctctatgc gaattgcatt ccaactggca
     2101 atgatgtgta tacggtgaat ggattgcctc ctggtgcaac agtaagattt gatgcaaata
     2161 atcgacgatt tatcattgag aatattaata caccgaaaag agtgcgtaat gtgactatta
     2221 ccgcaacgga tgaaaacggt gttagcagaa cttcaaatgc aattactatc cccagtattt
     2281 tataaaaagg gctcaatatg aatagatatt ccatattttt tttaattttt tgtactttat
     2341 cactggaatc tcatgccagt ttgtattcag gaattaattt gggaatcaat gctgttaaaa
     2401 tcaataaaaa tctggtctac ccgctggaag atcctgtgcc aacaacttcc agtttcaata
     2461 gtgcttatac tgattttcat ggacaattga ttgcaggtta tgatttttct cttgccccaa
     2521 aaatatctgc tgcagttgag gcaaatatag acttgtttac aggtaaggca aaacacagaa
     2581 ttaccgaatg gtattttaat gaggatgtgt atgcagaaga gcagttaaaa gtaggttatt
     2641 cgttattttt gttacccacc tatcatttaa ataacatggt tcaatttttt gtaggtccag
     2701 gcgtgtcatc gagtcgattt aatattgctt ttagtaatac tgctggaaac gtaggggtca
     2761 gtgatagttt gaatgaatgg ctgaccggga taagtattaa ggcgggttca ataacaaagc
     2821 taactcgcaa tatagactta cttttaacct atcaattcac acaatataac agtgttaccg
     2881 gggttcaaat ggaaccttta tccgaagata cgttgagagg gagctataag ccaaacgtaa
     2941 acacagtgct aataggtttt aaagtacacc cttcagatga aatcacaaaa gacacttaat
     3001 ctcagttatg gataagacct caattgcagt tatttgaggt ttcctatcct taactgattt
     3061 taaataatca aactgagcac gccatgtcgt ggccgcagca aacaggtgat ttgatttgac
     3121 cgcatccgtt ggggcatttt gctacttgaa ctttattccc atcatttttg actatggtgc
     3181 cactaaccaa caatgcgtca catttactgc aggttaaatt ttttacgccc ataccacatt
     3241 gattgcattt acattccatt ataatgactc cctgttattt aagtagaaaa acttttatta
     3301 attataatcc atactaaatt gaattggatt cttcattaga tttctgtcct agcaagactt
     3361 cattgacgct ttctctaata ctttctgtta ttgcactaag gtttaagtga tcttctgtta
     3421 aaccaaatgg ttcttctaaa tttcgagcaa ttccctctat ggcgatgatt aaataggtaa
     3481 taataatggt agcaggtatt gcccaaactc caagcgtatc gattaggctg caaggtaaaa
     3541 aaaggataaa aataatgaat acatgcttta caaaaacccg aaatgaaagc gagataaatg
     3601 tatttttaat acgctcgcaa ccgccgcaaa tatccataaa tttgctaaat tcgttatcca
     3661 ataaccatag attcaatctt ttcgatgctt tggtcttctc agaaagctca cgatacagtt
     3721 cttgtgcaac ataagacggc acatgattgg gaagactcac catttttaaa aattttttaa
     3781 aatcatcccg agtacttttt tgcctcaaat ggatggccaa tacttcacaa aacgtattga
     3841 tgagttcaga gaatttttta gcttcatcgg cattgggtga taacaatatc ctgattttta
     3901 ttgctaaatt acgactggca ttgactaatt gaccccacag ggttctcgct tcccaccaac
     3961 gttcataagc ccgattgctt ctaaaaacca aaagcaaacc taaaatgatg ccaaatacgc
     4021 tttgcgaata ggatagccag ttactggaat gcaagttatc ttgctcaaac caaaaataaa
     4081 taatggcgct gtaaacacca accaaaacgc taattccaag tactttcttt agggcatggg
     4141 tgaaatgtac ttctgaaaat aaatagctaa ccatggcagt ccatttgatt gtttagtttt
     4201 tccagaataa tagaaactga tctttctata aagtgctttg tcaaaaaagt gggagcaaaa
     4261 taagtaataa ccagagaatg ccagttatta cttattgttc actctaaatt cttaaagagc
     4321 aatagtctgt ttattaacag ttaccaccat ttatgggtgt tgatgagtat tgggtgctgg
     4381 ttgttggtga taatgttgat tctgggatgc gtaatagggt gaatgatgat gagttgtaaa
     4441 aaaactattg ggatggtaca taggcaattg ccctggaaaa tgaggagggt tcggattaac
     4501 aacgacagtg gtttgtccct ggctgacttg tacagtagca tttgatgggt gagagtgtgt
     4561 tgcagggtga tgatgtactc gtttcataga ttattatcct tataaaaata caactgcatt
     4621 acatacataa aactgacgtt attcaggcta ttcaaaaata acctgagtgc gagattgtaa
     4681 tgtagatagt taaacaagta cagatagtgt taaaaattta ctattttcac ttcctgttgc
     4741 ctcaatatta ttagcttttt gtgctataat tatgcgcata gtggttatat aaacacactg
     4801 ttctagccaa gacaattaac ataacttaat aagctaagga ataagcacca agagggtgat
     4861 ttgtatttct ttgtgtttaa ctaaatttat taaggtcttg ttatggattc tctagattta
     4921 gatttttatg ccattaccag agctttttta tttttttcgc taattggttt gatggtagtt
     4981 cttgaatatt tctttagttt tcatcgtttg ggtaaagcag gggttagaag gcgaagagtc
     5041 agcaattggt atttgggaat cgttaacatt atagttgccc ttccattaag caccttaact
     5101 acagcggcct attgtgagta ttaccaaata ggattattta acttgctgaa ggtacctttt
     5161 gggcttgtct ttattttatg gtttataata tatgatttgt acgtgtactt atttcatcgc
     5221 ctaagtcatg caactttctt atggcatttt cataaaatac atcattccga caaatcgctt
     5281 aacgtaacaa cgagttttag atttcatcct gttgaatata ttttattcaa tgctttaaaa
     5341 atagttttga ttatcctttt gggtccttat ttctttgtca tattagtctc cgattttatt
     5401 caagcgatac ttgtattctg gggacacagc aacattgctt tgaatgctaa acttgagcaa
     5461 gcactatctc gaataattat tactccgaga tatcatgtga tgcatcatac gcaagatgaa
     5521 tgtagaagaa attatgctgc aggactaacg ctatgggact atttatttca tacccaaaca
     5581 gaaccccttt ggaataaaga tgatatcaat aaactaaccc tcggagtcca aacaaaaaca
     5641 aattggtctg aattcaaaaa tctcttatta ataaataaat taatccattt tttgggcagg
     5701 ctacaatttg tctatttcaa attcccccta attacgatag gagttgcagt aatttttgga
     5761 atagttgaat tttattaatt accttagcat gtaggcttta gttaaaaata tagccagttt
     5821 ttaaaaactt attttaggga accataacta atgattccct atacatagat tgttatcaaa
     5881 ttttggtagc ttctagttat gttaaaaaga aatttatttc ccatgctgaa aagaagtagc
     5941 cggttgatgt tgtctggatg tgtcttcacc atggcaaagt ttataaaaaa aacaataaag
     6001 attataataa ggtgatgcac caggaataaa atcgggggca ttatttcttc tcacttcctt
     6061 cttttgttct tgttccgaag ttggtttatc agtctctttt ttttgcatgt tatttgttga
     6121 tttcataaat cttatccttg attaagaaaa tagattgaat tttaagtatc gttcaaacca
     6181 ggaaagagag ttccggtttg tttacaaatg aaccgtttta ttgctcatgg cttggtgatt
     6241 atagttcaga caggtgatca attgtagtag ttgtcccaga tgtgttatgt ttatctgact
     6301 gacaggaatt gaggtgccag aacaaggaat agataatgaa ctgttttcct ttctttgcaa
     6361 aaaaaaataa aaaaaataac gaagaaaaag cggaaaaaat attcagcgta tttcctgcag
     6421 gtacttctat aaataaaaca acggtaaagg aaatttgttt tacagatatc ttttgtgaca
     6481 cccatccttt tgtttggtat tactatccag aacataatct ggcaatttgg acttcaaggc
     6541 tggcagggac aacccaagaa tatccggcag attatttgct taatcaactt gcaaaaattc
     6601 ctggaattac catcgaccaa ggtgatgcag ccatcaagga tgttagtact ttcctccagg
     6661 gccatcaaat gattcctatt agaaacaacg aaagctggcg gtatatcaag tcttcttctt
     6721 tctcaatgag ttaatgaata gattgaaagc aggtccttgt tcttgataat ggaagatttt
     6781 gctggctcag tttcgcaagt gatgcgcaag ggatattcaa atgatttctc tcatcttatt
     6841 gattgcatag agcccacaca taatgaaaca ttagaaagtt ataatcataa taatatacaa
     6901 tgagctcatt taaattccta aatgagcaag acatgaaacc tactgataaa aaacactggt
     6961 ctaaaatcga atatctacac caaagcgtaa aaaatccaaa catccatatc aagggtaaac
     7021 acagttatta tagcgatgcc tggactggta gttttgagga aaccgtcgtt cggtatcttt
     7081 atggagatga atacagttta caaacctggc aaccacaatg gcagattgat caattatata
     7141 ttggtgatta tgtttgtatt gctgctgaag tgataattct gttaggtgga aaccataatc
     7201 accgagcgga ctggttttgt ttgtatccat ttgcagataa atacgtagag gcttatcaag
     7261 gcaaaggcga tactattatt aaggatggtg tctggcttgg catgcgtgct gttattatgc
     7321 caggcgttac tattggcgaa ggagcaatag tggctgcaag cagtattgta accaaagatg
     7381 tggagcccta cagcattgtt gctggaaatc ctgcgaaacc agtcaaaaaa agatttgcag
     7441 aaaacgtgat tgaacgaatt ctggccttag gtatttattc ctggagcgaa gccaaattta
     7501 acttattaaa agactatatt tgcagcaata acatagataa attagagcag gctagcagga
     7561 gctatgatca aaaccatacc atagaaagca aatgataaat atcaggaaat taagattagg
     7621 tgatgaatcc accttggaaa cttttttaaa atctcatgct gatgagagta tgtttttacg
     7681 cagcaatctc tatcattccg ggttgtctta tcatgataag ccttttcatg gggattattt
     7741 tgctgcattt gataccaaca aaaagataag cggtgtatta gcccattatt ggaacggcaa
     7801 tgtcattgtg cagtgccctg atttcaatgt tttaacagca ctagtgggca cttttcaaaa
     7861 agccgctacc cgagaaattg caggtattct cggcgaagat cagcaagccg atgttgtcat
     7921 gaaacaactc cagctggagc atgtagatta tgccgtgaat tacccggatg ggctatacgc
     7981 acttaattta cagaatctag caatgccagg taatcaagac agatatcaac tgaaagtcgc
     8041 caaagactgt gataaatgtt tattatatga atggcttaga gcttatcaaa tcgaagcctt
     8101 aggtgctgaa gaagacagtt cgattttaaa agagcaagta gagagtgaag tgaaaagtac
     8161 tctcgaaggc aatgatcgtt gggttttaga ggagaacggc acccctgtct cgctatgcag
     8221 ttttaacgca agattatcag atattgtcca ggttggaccg gtatggacac cagttacgtt
     8281 gcgggggcga ggatatgctc gaacagttgt tgcgcttagt cttagtgcag cccaaaaaag
     8341 tggagtgagt cgagcggtat tatttactaa taatcaagct gccgtacgag cttaccaggc
     8401 aattggtttt aataaagtag gcaattatcg attggccata ttaaaaaaac caatactctt
     8461 gacacgttaa ttgtgaatac ctgttaaaac agtaaaagta tttattgata ggaaataaaa
     8521 cgatggatat ggatattaaa cataagcctt tgaagcgtga actcagtcta tttggcgcta
     8581 cgatgatggg gctcggctct attgtcggga cgggtgtttt tgtcagcatc ggaattgcgg
     8641 cgggtgtcac aggaccatcc gtcattcttg ctattgcttt ggcagcactt gttgctacct
     8701 gtaatgcgtt ttccagcgct caacttgccg caaatcatgc tgttagtgga ggtacttatg
     8761 aatacggata tcgttattta catcctgctt ttggtttcac tgccggttgg atgtttttat
     8821 gcgcgaagag tgcttccgct gcaacggccg cgcttggctt tgccggttat tttattcacc
     8881 tgtcaggttt agagacagtg cctgtcatac ctatcgcttt agcagtgtct atcacactaa
     8941 tacttttagt cttaagtgga ctcagaagaa caaatacagt gaatattatt attgtaatga
     9001 ttacattaat aaccttaacc gtttttataa taagcggatt acctgatttc atgcaaaggg
     9061 gaactgctaa tctgaggccc ttctttcctg tttcaaaaca agaaggatta agtcattttt
     9121 tttatgccac tgctttaatg tttgtggctt ataccggtta tgggagaatt gcaacattag
     9181 cggaagaggt taaaaatcca ggaaaattta ttcctgcagc gattattatc actctgatgg
     9241 tgagcgccgt actttatatt ctggtgggtc ttgttgctat tggagctgta ggggttgaaa
     9301 aactggccca agtgactgaa acaaacgcaa ctcctttaga gagcgcggca cgagccattg
     9361 gtatccccgg attgagtgtt ctggtctcca ttggggcatg cacggccatg ttaggggtat
     9421 tactcaattt aatacttggg ttatctcgtg tggctttagc tatgggtaga aaaggcgatt
     9481 taccgaagat gttttgctat gtatcagaaa caaggcatgt tcctgtttcc gctgtcattg
     9541 gagttggagt tgtaattatt gctctggtat tgactggaag tgttgaaaca acatggactt
     9601 tcagtgcgtt tacagtttta atctattacg caattactaa cctcgctgcc ttgcatttgc
     9661 ctaaagagga gcaattgtat ccacctctgt ttgctatcgg aggcttactg tcctgccttt
     9721 tcctggcgtt ttgggttcca attggagtat ggataacggg aatcagttta cttttcctgg
     9781 ggcttctttg gaaagttgtt gccaatcgtt ttttataaag gcaagaaaaa aagacggatg
     9841 tattctagaa taaacaagaa gatcagagaa aaggaaccgc aatgatcgat ggaattatgg
     9901 ttttatggtt tattttactg atgctctctt tggcttttat tgcttatgat gtgagggaga
     9961 tgccaattga ttgggtgcaa aaactaggat gggtattggt cgtggcttat accggccctt
    10021 taggcttgtt tttttatttt ttgacttgca gaagcccagg taaaggattg catgctttat
    10081 ataccaaagc gcaatggaaa caggcggtta attccgaagt tcattgtctg gctggtgatg
    10141 cgacagggat catcttcgcg gcaatttttt tatcttttat tgatttaagc aatggtgtag
    10201 agcttattct cgagtatatt tcagcctttt tctttggttg ggttattttt caggctggaa
    10261 tgatgagaag tatgtattca agctatggag aagctttaag gaaaacattt tttgcagaaa
    10321 ccatatctat gaattttgtg atgatgggta tgattcctgt gatgatgatt ttaatgaaca
    10381 cattagacta tggacgcaac ccggcacatt tgcaattttg gtttattatg ggaatggcta
    10441 ctgtagtcgg tggttttctg gcttttccaa ttaataactg gttggttaag aaaaaattaa
    10501 agcatggatg tatgactttg ccagaggggg acgaacaaat cgaagggcat atgacccatc
    10561 atcatggagg tcatgaagag catctacagg cacaagctaa aagaattggt tcgaaagtca
    10621 ttcatggaca cacccacgaa atgggaagct taccattaaa cattcaattg aaatatatcg
    10681 gtataagttt tggagtttta ttgattattg ttttgataat ggcctttttt gttccattga
    10741 aaatataaca gagattttta ttaaatctga acaatgttgg attaaaataa tccaacattg
    10801 ttttcgtctt attgcatggt tcttgatatt gcttttccag ttgctttaat atcttgttta
    10861 gctcctgtta cagtcccctt gacagtatgg catgcagcta atagagcaat gctgatactg
    10921 agagccccta acttgattaa attccttttt aagttaaaca taactcctcc tttatactta
    10981 taattcattt gaattttaaa cgcccgtttt ttacttgctg ctgtatgtct tggacaattg
    11041 gaggcacgca ggttgttaaa cgagccatat aaaaatcaaa taattacgtt ggtattttta
    11101 gttaattttc ataaaaagaa ttctttacat aaagaataga agagtattct taattttcaa
    11161 gatacaattt ctatccaatg aaacagtcgg gatttacgaa aaaagtaatc gccagaattc
    11221 gctcttgtta caaaattatt tgtaggtaat aaaactgata cgccccgttc gctccaaacg
    11281 agcttctttc actgttgtca aatcacttgc atttaatttt tctctgaaaa cctgcctcaa
    11341 atcttcttca gtcacttgat gtcttttcaa agcactccaa atgagctgcc catcttgtat
    11401 taatagcacc gatttccctt taaccagttt tccaaatccg tgggaataaa aggaacaaag
    11461 agcaaagatc caatgtaaaa aaataagtaa actgctccca gccattgcag atattaaagt
    11521 agagttgcca ttaattgcac gacttaacac ggcgccaatg acaaatacaa aaatataatc
    11581 aaatgctgtt tcaaaattga atcgggtatt gccaacacgg atgataaaaa ttgcgaaaac
    11641 gtagataata aaagttctta taaaaagata taaaaggttt ggatcggaat taatattatg
    11701 gaggtagtca agccacatat ctgtttcaac aaatcactta tcccaaaact ataaatagaa
    11761 ttgcttttct tttgcaataa ctttatcaaa ttttaaatgg attgcttttg aatgcctgtc
    11821 tggatagaga gttaatctgt tgtttataaa catcaatttc ggcagataat cctctatccg
    11881 ggcatataaa accttatttt ctcagattag ttctgataat taattgatat tcacgtcgtt
    11941 ttttctttca taaagagttc attaagcttg tcttcgtaaa aatggtaatt taatacctga
    12001 tacaactctt cgcgagtttg catctctgcc agaatatcgt tttggctgcc gtttttgcgg
    12061 attgtgtcgt aaactttttc tgcagcggca ctcattgccc gaaatgcact taaaggataa
    12121 agagccaggc taactcctac ttcctctaat tcttccagcg taaataatgg agtaactcca
    12181 aattcagtga tattggccaa taccggaaca ttacattgct cagttagcgc ctgatattcc
    12241 tcaagtttac gtactccttc gaagaaaatc atatctgccc cggcttcaat ataagcacta
    12301 attcgctcca gcgccttatt taatccttca ttcgccaggg catcagttct tgccataata
    12361 acaaaatcag gatcagtttt cgcatccaca gcggccttaa tacgatctat catttcttct
    12421 ttttcaacca atgccttacc cgggcgatga ccacatcgtt tcgcttgaac ctgatcttca
    12481 atatgtaccg ctgctgcctc agctttaatc atttctttga tagtccgtgc gatactaaag
    12541 gctccacccc atcctgtatc aatatcgacc aggaggggta aatcaacggc actcataatg
    12601 cgccgtacgt cttcgagtac atcatttaat gtggtaatgc ccaagtctgg taagccgtaa
    12661 gaggcgttag ctactcctgc tcctgaaaga taaattgcat gaaatcctgc tcttttggct
    12721 tgcaaagcgg tataggcatt aattgttcct acaacttgca atggtttttc attcattaac
    12781 gcttcgcgta gtttttttcc ctggctgtat cgaaaatttg acatttggtt tccttgtttt
    12841 gcctaagatg agaatttatc aggacttacg aaaaatccca atctctttgt taaaaaaagt
    12901 ttttaattcg gtcatttagt ttatctaaat gagagatggc aaaaatcaat tacaaatcat
    12961 gaataaccca aacatttggc tcaatgtata ttccaatatc cttttcttta ttgatagtta
    13021 taggatttaa tccgccttta acaagatatt gacctgtttg aagcaatgga tgaccgagcc
    13081 attgttccca ctgagcaaca ttcgctgtga tcacgcgtga tcgctggcat ggataagcta
    13141 tttttcctcc cagttgtgca tgaactctta accaaggatc aaaaatagta ttatccactc
    13201 ttttccactt caaataatcc tctagggaaa taagcgggta gttttctttt tggctaggtc
    13261 tggctggcac tattagtgct ttatattgag cctcctgtgc actggctttt aaccgattta
    13321 ataagagttt gcttaaacca agtctttggt aagccggatt cactgtcact gatacagcag
    13381 caagataatt tgattgggtt aagggtgttg tagattccag atgagacagg acccaattta
    13441 atccttcttg tggcaaatta tcgagtgatt gatcaagcgc tactggcaaa gaattaataa
    13501 cagctactac ctcaccatta tagtgagata gccattggta taaaggataa tgactataga
    13561 gcttaaaaag agtgtcattt ttgatgcgtt gataatcagg ccaaatcttt tgtcgtaaat
    13621 gtagaatttt ctctatccag tcttcttgcg ccttgccagt tagccacata agtttgttaa
    13681 gcatgagctt gttccaaaag gtgtgctttt gcgttaatta aaccggacca ccaatcttcc
    13741 attatatcga tcgccgcata cgcgccatcc attatttctt tttcctggag aaaaatattg
    13801 ggatcaacgt gctcttcagc aagcgtatga tccataaact tgcctaactc atctttatgc
    13861 gcctgttcta ctccatccag gtgtaaatca tggaattgct tgatgcgaga attctcaaaa
    13921 tccaaacctt ttctttgaca aagctcggcg cttatttctt taaataaaat atgctcaaat
    13981 attgcagctg tttcagtagc gtacataacg ccaagggttt tggatgcgtt aatcacaatc
    14041 aaggcttcaa ggcgcataaa aaactgtctg gttgccacat acggaatcct gtttgttaca
    14101 tccaggccga ttttttttag tgcatgccga tacatttgag catgattttt ttcttcgtta
    14161 tagttagcga gtaactcttc agaaactccg ggatcagaaa gcgacttggt ttggatatga
    14221 gaattcatta aaaaacgagt attatttaac gaaaactgat aatgctccat ggttatccat
    14281 tcaagataac tcgtcgaaaa tttttttagg tcactaaaat aatttaaaat ttcggtagta
    14341 aattggttgt ttctcttatc catgtattct cttatcatga agcctccagt cagtattaaa
    14401 atcaattcca agggtttacc ataactataa agatcatata attaaaatat tattttataa
    14461 ttatcttgat aatttttagt tatgataaat ttttcacaaa tccaggtatt tattacaatt
    14521 gtcgaatctg gtggatttat tgctgctgga gagaagatgc atcgttcaca gccggcaata
    14581 actaatgcca taaaaaaatt ggagtccagc ctgggcttcg aactgtttga tagaaaagta
    14641 taccgacctc aactcactgc gaaaggtaaa gctttttatc aaaaagcaag agaagtggtc
    14701 gctcaattta tgcaactgga aaatagtgct gaaacattga gatccggagt agaaacacaa
    14761 ttttctatag atctcgatat tgctttacca ttgccaggat atgctccgct tattcaatct
    14821 ttcataagac aatatcccga aacttgtttt tcactaagta acaatacttt tgttcgatgt
    14881 cttgagcgcc tggaaagaag agagtgccat ttatgtatag ctgcaagcca tattcgttct
    14941 ccacagatag aatatttgcg tatgccttcc atacgaatga ttcctgttgc gagcagggac
    15001 tattatgacc agcataaaga aagaataaat aatcccaagc tgggaatata ctgtatgcaa
    15061 attatgatag ctggcacgca acaggaatta tatcaactca agattgagcc attaagtcat
    15121 tctatgccca aatggttagt gggtgatatg tatattcgta aacaactgat tttatcgggt
    15181 atgggtattg gccgtttacc ggatcatctg gttgaaaagg agttggcaga gggctctttg
    15241 gttcagttaa ataccaggga ttatgtagcc gttactatgg atatttatgc tatgcggtta
    15301 aataatcaag agcatggcat ggtctccaat tatgcttggc aatcactaaa aagttatgct
    15361 gaagacacgg tatctcccga tttgaaataa ccaatttttg cgtttgcttg ctaattcaag
    15421 cagtttggtg gatcgaatat ttgaaaaaca ggaggtgatt cattgacata aaaccaagat
    15481 aagcctaaat ttaaatcaaa aagacataaa attgatctcc agttggatat tataatgatt
    15541 gattttaaaa taaattccca acctgatgat gaatcttgcg ggccaacctg tttacatgcc
    15601 atatatcaat attatggctt ggatatcagt tatgaagagg tggcaaacca ggtagagagc
    15661 tttttatctg gtggaacaat agcgcccatg cttggtaaac atgccttgca attgggattt
    15721 aaagtcaccc tttatgtcaa taatttacaa ttgtatgacc cgacatggtt taatagcgat
    15781 ggttcatcaa ataccgacgt tctgagagaa atgctcatgc aacaaatgag gtataagcgt
    15841 aaccagtatc ttgcccgcgg aacacatgca gcacttgatt ttctgacttt agggggcgct
    15901 atctattttc gatgcctaac tgcagaaatt ttaaaggaat attttgccag gcagattccc
    15961 atattgacag ggcttagttc aacctatctg tataactgca gtcgtgaatt attcaacaat
    16021 gaaggcaaat cgaggagtga tcctattcgc ggcctaccgt gcggacattt tgttgtactg
    16081 tgcggataca accaaaaaca tcgcttagta gtggtcgctg atccttacaa aaagaatccg
    16141 gtttcaggtg ataattatta caccgtatcc atccatagat tgattaacgc aattatgttg
    16201 ggagtagtga cgcgtgatgg taatttattg attattgaac cgaaaaactc gtagacgagc
    16261 agcaagttcg aactatccca gaccaagcaa ttgtattacc agatttttaa tattaattta
    16321 aataaaacag tgcgagtaaa tatcttcttt atcattcgct aataattcat atacaatgga
    16381 tgccggttaa tttaaagaat ataacgacac ctccaaaatg agccgagatg aaaattgaca
    16441 gctggtgagt aacgtaatag caaaagcttc tcatgaagaa attttggaaa ttatcaataa
    16501 tttaatggct ttctattttt atgggtttaa cttttctcaa aggtcataag tatggggcaa
    16561 ggtgaagagc tggtttctat acattttgtt actaagagtg aaagtctatt ccgagcttcg
    16621 gttaattcat taatttttaa attaagtttt tcaatacgcc tttcagccat atctcttacg
    16681 cgctctcgat catttttagc atcaaactcc aatagttctt ttatttcttt taaagtaaag
    16741 cctgcttttt tggctaaaag gataaacttt aagcgactta tatcatcgtc accataacgc
    16801 cttatttttt cagctccaag ttctttgtta agcaacggaa catgaagcag tccctggcgt
    16861 tgataataac gcacggtttc aatgccaact ccacataatt tagcaaattc accaattttt
    16921 ttattattca ttacttgact ccgtactata gtacgtactc tatattacta tttatagacc
    16981 attaatcata ggggatagaa atgaatcata cttcaattaa aaaagcagtt ttgtatcgca
    17041 tggtgataga acatcatgtt tgcccttatg gtctcaaagc tagagattta ttaagacgtc
    17101 aaggctatga ggtagaagat aattggctaa caacccgtga acaggtcgag gcgtttaagg
    17161 ataaacacaa tgtgaaaacc acacctcaga cttttatcaa cgatgagcgt atagggggtt
    17221 atgacgattt acggcgttac tttggaaaga aagtaagaga tccaaaagaa aaaagttatc
    17281 gccccgtcat cgtactgttt gcgattaccg ctttaatggc gatggcagtg agttatactc
    17341 gctttgccaa tccttttaca ccacatgctt taagctggtt tattgcattt agcatggcag
    17401 ttcttgcttt attaaagctc cagaatatcg aaagcttctc aaccatgttt ttaaactatg
    17461 acctcctggc caaacgctgg gttccttaca gctacattta cccatttgct gaagctttag
    17521 cagggatatt aatgattgca ggagcgttaa attggctttc tattccaatt gccttattta
    17581 ttggaactgt aggtgctctt tctgttttta aagccgttta tatcgacaag cgtgagctta
    17641 aatgtgcctg tgtgggaggt gatagtaacg tacctttagg atttatttct ctcactgaaa
    17701 atttaatgat gataggaatg gctttattca tgttgcttat gtaattcagc tagcaagcgt
    17761 agctctgatg aagtggaacg aaatccggga gtatggctgc gcttgtatca gagctacact
    17821 agctatacac tgtcaacata taaaaagtcg atcatcgagc tatcgatatc cagggttgtt
    17881 aaagttctct taagatagac cttatataat gaacattttt tatatttatt gcagttctgc
    17941 cagatgagca aagaaatcaa gaagaaccta cagaacaata attcagcctt tgtctacttg
    18001 cgcagcacgg ggctcgccac aaactctctg tcgagcattc tgcagtacat gacacgcatc
    18061 attgtactta cggaaagcgc gggcattgcc ctgggcattc tgggagtgct cttttcagtc
    18121 attggttttg taagcagtac tattgcaaga atacttgatc caacagcctc taaattaagt
    18181 cgtgtgatgt cggctggtat tctcattgca gctgcagcag gggctatacc actcatctat
    18241 ctgggaacgt tattccccgc gatgtgggta tatcttggta taggcattat ggggtttggc
    18301 atacttaatt cgtcatacta cctttattcg gacgtgcgta aggaaagtgc actcataaat
    18361 cagtataaag ataaccctga acaatatgtg gaaagtatta aagaaaagcg acaagccaag
    18421 tacgaaaaac ttctcaacac gcggctcgac gataagcaag cacgagtgct gctagttttt
    18481 aagctttacc aacttgacaa gaaattagcc tttgctaaac aagaggggcc agctagactt
    18541 tacgacatcg tatgccatcg caagcacacc cgtagtctgg agagatttat tgaccttggc
    18601 acttctctta caagccttat tgttggggtg tcaggcctgt tttggggaat cgctgcctgg
    18661 ccaattggcc ccatattgct cggtatatct ttggctgtat tttctgtaag cctatcaaag
    18721 tcagcgatac agttttatag aaaagttaat aaaaacaatg cgcttttaaa agaagttctc
    18781 tcaaatgata gatatgtatc acttattgat gcgaatctgg acaacccgta tgacatcgaa
    18841 gagatgggca ctgtggaaaa aacacaagag aaggtttgta cgcatgagct ttcacaagag
    18901 tctgcgctct cgaaagacca agaacataaa gaacattata ccaaactctt ccagcctgtg
    18961 aataaaaccg ctctcaggcc aaacgatgaa gagaccgatg agctaagctg caagccggtt
    19021 ctactccaaa ggtagtggca ctataatctg gttatagcaa tctaactcct tgactccgca
    19081 atcaaaagag gattaatgag tatatttttc caggcacaag agcagcaggt catatcattg
    19141 aaccacgtaa gcaaatggcc aatgtgacca aactctcagg cattcatttt acggatcatg
    19201 atttaagacg aactcttatc actattgccg aggggctgga tacctcggct tatgctttga
    19261 agcgtttgat gaaccataaa atgaacggtg atatcactgc ggggtacatc gttaccgatg
    19321 ttgatcggtt gagaaagcca atgccacaaa ttaccgatta ttttttaaag tgtatgggtg
    19381 tccaaccatc cgcaacacta gtagcaattc gaccacaagg agcagttcat gagtaataaa
    19441 attaaagttc aagatgacgc attaccctta aaccaagatt aaatttcttt aattgacaaa
    19501 tagaagtgaa gaatatccaa atagtaattt atattgagcg tttcttgatt atacgtaatg
    19561 atatcaatct attgattttt ccgagggatc tatttcatat ggttttttca ttctatatca
    19621 cttatactct tttatagagg gcatataaga gtgaatatta tgagcatctt gattaaattc
    19681 gaaagcttgg ctaaacaagc aatcttttcc agtaacaaag atatcattct tcaatcgatt
    19741 ggcctgcttg atatgattaa ggctaaaaat aatcaaaaaa taaggatgac tttatctaat
    19801 agttctgaac aatttgctga tatgtctagg gtagttagat agtaaaagga tttatcatta
    19861 agaaattagc tttagttggt tcaggtatta aatctatatc tcatttcaca tttgaattta
    19921 atacttacac taccagtgca gataaggttt tgtatttggt taacgagcct attactaaac
    19981 aatggataga acgttattca aaattgtcag aatctcttga tccgatatat tttgctgaaa
    20041 atgatcgaca gatttcttat gataaaataa gggataaaat tcttactgag ctggaaacac
    20101 ataattttat tacggtggta ctttatgggc accctacagt tttcgcagat cctggcttac
    20161 agtctataat tatagctcaa aaaaaatcga ttgaaacaat agtcttacct ggtatttctg
    20221 ttgaaaattg cctctatgca gatcttaaaa ttgatcccgg acaattcgga tgtttccatg
    20281 tagaggcaac agaattattg ctatatgata aaattatcga tcctacggta cacctttgta
    20341 tttggcagcc aggtatgatt ggcaatcgct ctgtacctaa accaaatcaa aaaagtaatc
    20401 atttagagtt actaagaatc aaattaaaaa aatattaccc agacgaccat atatctattc
    20461 tttatgaggc gtcaatgtac ccaggtgttg aaccaataat acataaattc ccattatatg
    20521 atattgaaga tcaaaacatt ggaacacttt caacattata tatcccccct cttccccaaa
    20581 ggaaacctaa tttagatatt ctgaaacaaa taacctcata gaataagcta ctaattattt
    20641 aaggaaaaat atgttatctg tactgtctta tcgattaagt ttactgccaa aattatcaaa
    20701 aacttgctat ttacttcttc ttttttcttt cttggaatgt gtagcaggag ggattgcata
    20761 ctatctatca ttatatttag gaacatcaac acccttaaca aaattacaaa ttggtcaggt
    20821 tggttctatc gttggatttg gagcattagc tggtgcgtta tgttctactt acataacaga
    20881 taaactcaat aataaaaata ttataattgc cagttttctc tttctaggtt taggcttttt
    20941 ttgtttacct caaagcatga gttttctgct tatatcaggt tgtgtcttca taattggttt
    21001 tgcaacaagt atttttctta ccacgaacaa taccctctta ttacaaaatg cccctcaaga
    21061 tcaaaatagc cttcgcttta tacaaagtat gaaaatagtc agtgaaaacc ttggtaatag
    21121 cttttctata ttattaatta tgttttttgc agcgcaatat tacggtcaaa tttttgaagt
    21181 aattggtcta tttttcttcc tttttgcact gaataccatg aaatatttaa atgtaaagca
    21241 aaattcatta gaagactcct cttatgaaga aaaagaaaca aatcaacaag ggaataataa
    21301 acgactttat gtaactatta gtgctgtatt tatagttgga attatctatg cccagcaacg
    21361 aattgcatac ccactttttt tacatgaagc tttttcaagt tttactatca ccgcaatact
    21421 tttctggtta gatcctatta ttgtttcttt atttcaggtc aaaataacaa aaatagtatc
    21481 cccattgaaa atatctcatc aattagcaat aggagggtta ttattaggta caggcttatt
    21541 tgcattattg atagtcgata gtattggagg tgcaatcatg gcgtgtttga cgtttatttt
    21601 aggagaaatg ttatttatgc ctacttcctt tgtacgatgc tatgagtcag ctggaaataa
    21661 gcgtaaaggc atggttgctg gtgcatggag aagtgcttat tcctctggtc tgattgttgg
    21721 ccctgtttta tcaggtttcc tcatggagta ttatagttat aatgcctgtt ggatattagc
    21781 cggaatactt ggttttttcc tatttatttt atttagcgtc aaaaaataat taaatactat
    21841 ttgaaaaaat cgaaacgcat cctttaaaaa aggatgcatt aatcgggaac ctcttttcac
    21901 ctctgaagcg ctcatcctga taagggataa tttccggtga tttgataatg ctattttctt
    21961 cagtagctgg atgatagaaa ttaaaactgg ttatttttga aacctcttca aggatagtat
    22021 ttttacattc ccgaatcggt aaagtgaaat ccttttgata tttatgaaca acatcaatta
    22081 aagcgataag atctgaaagg gtggtcgatg cattccttgc tttgggtgaa taactacata
    22141 gagagggata ctgaagagaa taataaacag gtttaggatc ccggtaatta taatatgcag
    22201 gtattgctat aatgggcaaa tattctaact tataacattc tgaataaact tcttgaataa
    22261 tagataatgg gagtaattca tcatccattg caggcgcaaa accaagtttt gcacctagca
    22321 gtatctcaaa aagatgtcgt gctgtttcat agattaataa attggatttt aaattattcc
    22381 ttttattggt aactctataa acgtgattat aataatcgct atttactgca tattcagatc
    22441 gatggtttga aatcctgtaa aaataatctc taacttcctg ccatgcggag tccttactga
    22501 ttttttcaat ccaattttca gaaaaatata atacagacgc actccaacca ttattatccc
    22561 tgttaccttt aactatatct tgaaatatat cagcatgctc gtgataagag cgaggagccg
    22621 actttgtaat attatgattc ttttgtattc gcgaatgcat aattccacac ccaattttgg
    22681 gaatcataaa attggatttt gaaccggctg taacaaaaag aaccccattt ggtaaatggg
    22741 ttgctgtgta accataatta ataatatgtc ctatactaaa aaatgtacct ggaggatcaa
    22801 tatatatagg aaatgtccgg ttaatttgct catcggaggt atttaaaaac cattcaaatg
    22861 atttattaaa aaaaacgcct aatggactat tagtcaaacc ataagccaaa tcatttaaaa
    22921 tcgatggggg agtatattct gaatcaagac gaacatattg acctgtttta gttggcaaaa
    22981 atatcccatc atcatcacca accaaatctc cataaccata atctacgaga tacattgcaa
    23041 atttattatc tggagagagt ttatcaacta gttcaaaaaa aaccggatta atggttttca
    23101 tgatgttcct gatatcagtc caaaaaactt tttttattgc taacatttt
//
LOCUS       DACTJE010000036        22443 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.036, whole genome shotgun sequence.
ACCESSION   DACTJE010000036 DACTJE010000000
VERSION     DACTJE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22443
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.036"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(39..1829)
                     /gene="oadA"
                     /locus_tag="I9760_001087"
     CDS             complement(39..1829)
                     /gene="oadA"
                     /locus_tag="I9760_001087"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HAT5042863.1"
                     /translation="MSKTFITDVTLRDAHQCLIATRMRTEDMLPICNKMDDVGFWAME
                     VWGGATFDACLRFLKEDPWSRLRQLRQMLPNTQLSMLLRGQNLLGYRNYADDVVRAFV
                     KLAVNNGVDVFRVFDALNDARNLKVAIDAIKSHKKHAQGAICYTTSPVHTLDNFLELG
                     KKLAEMGCDSIAIKDMAGLLTPTVTVELYAGLKQATGLPVHLHSHSTSGLASICHYEA
                     VLAGCNHIDTAISSFSGGASHPPTEALVAALTDTPYDTELDLNILLEIDDYFKAVRKK
                     YSQFESEAQNIDPRVQLYQVPGGMISNLYNQLREQNALDKMDAVHKEIPRVRKDLGYP
                     PLVTPTSQVVGTQAVINVLTGERYKTITNEVKLYCQGKYGTPPGKISSALRKKAIGRT
                     EVIEVRPGDLLPNELDQLQNEISDLALSDEDVLLYAMFPEIGRQFLEQRKNNQLIPEP
                     LLTQSSAPDNSVMSEFDIILHGESYHVKVAGYGMIEHGQQSCFLWVDGVPEEVVVQHS
                     ELHDKIERSSVNNKIGPGDITVAIPGSIIAIHVSVGDEVKAGQAVLVIEAMKMETEIK
                     APANGVVAEILCQKGDKVTPGQVLIRVEVS"
     gene            2031..2468
                     /gene="aroQ"
                     /locus_tag="I9760_001088"
     CDS             2031..2468
                     /gene="aroQ"
                     /locus_tag="I9760_001088"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HAT5042864.1"
                     /translation="MKKILVLHGPNLNLLGSREPSIYGHASLTQINGDLIQEADNAGI
                     RLSCFQSNAEAELIQAVHQAGIDKINYIIINPAAFTHTSIALRDALSAVAIPFIEVHL
                     SNIFSRETFRHHSYFSDIAVGVISGLGTKGYLLALQAIIKELK"
     gene            2482..2964
                     /locus_tag="I9760_001089"
     CDS             2482..2964
                     /locus_tag="I9760_001089"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAT5042865.1"
                     /translation="MDIRKIRKLIELLEETGISEIEIKEGEESLRLSRHSNIVAETAP
                     QIHYVAPSAVKQTASAPSSNTSTTESHVIENKQVTSASSGHKIRSPMVGTMYTAPSPE
                     SPPFVTVGQSVKAGDTLCIIEAMKMFNEIEADRAGKIVEILVKNGDPVEYDQALFIIE
                     "
     gene            2977..4320
                     /gene="accC"
                     /locus_tag="I9760_001090"
     CDS             2977..4320
                     /gene="accC"
                     /locus_tag="I9760_001090"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAT5042866.1"
                     /translation="MLSKIVIANRGEIALRILRACKELGIQTVAVHSDVDKDLLHVRL
                     ADETVCIGPAPAQKSYLNIPAIISAAEITDAVAIHPGYGFLSENADFADIVEQSGFRF
                     IGPRGDSIRLMGDKVSAIAAMKAAGVPCVPGSDGPLGDDDNKNLELARKIGYPIIIKA
                     AGGGGGRGMRVVHSESNLLSAIALTRSEAKAAFNNPIVYMEKFLENPRHVEFQVLGDG
                     KGHAIHLGERDCSMQRRHQKVVEEAPAPGITPELREEIGASVIKACKELQYRGAGTFE
                     FLFQDGCFYFIEMNTRIQVEHPVTEMITGIDLIKEQIKIASDIPLTLTQKDIKLRGHA
                     IECRINAEDPRNFMPSPGTIKLLHQPGGPGIRFDSHIYSSYVVPPNYDSMIGKLISYG
                     ATRAEAIARMRNALDEIIIDGIKTNIELHQRILHDKSFMEGGTNIHYLEKMLKDS"
     gene            4322..5191
                     /gene="prmA"
                     /locus_tag="I9760_001091"
     CDS             4322..5191
                     /gene="prmA"
                     /locus_tag="I9760_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAT5042867.1"
                     /translation="MWFQLKIEHCPNDKIEEITEELEECGALSITLTDKNDNPVLEPE
                     PGTTPLWPEVIIHALFAQAEEAQYAREQLVAKRPSLHCSLELLADKNWERAWMDDFRP
                     QRFGNRLWVCPTWLPPPEPDAVNLILDPGLAFGTGTHATTSLCLTWLEQADLKNKSII
                     DYGCGSGILSLAAIKLGAKHVYAVDIDNQALQATQSNAHANHITESQLSISFPEALQN
                     PVHLVIANILLAPLISLKERFHQLLPSGAHLVTSGILEEQAPLLIDAYDSAFTHIATE
                     YCEGWSLLVFTSK"
     gene            5218..6807
                     /gene="purH"
                     /locus_tag="I9760_001092"
     CDS             5218..6807
                     /gene="purH"
                     /locus_tag="I9760_001092"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAT5042868.1"
                     /translation="MAKKQIYSSFKPRRALLSVSDKRGIRELGQVLHDQGVELIATGN
                     TAAILREHKLPVTDVSECTGFPEIMDGRVKTLHPAIHAGLLARGEEDSPVLRQHGIKP
                     IDLLIVNLYPFEQVINHPDCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYSK
                     LIHYLQDQKAPSHWNFALAKKAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFN
                     KITDLRYGENPHQQAIFYADKNSHPGSLSTATLLQGKQLSYNNILDADAALDCVKSFS
                     NEKSVCVIVKHTNPCGIALSDTSLDAYLKAFQSDPISAYGGIIAFNGTLDSDTAKAIL
                     EKQFVEVIIAPDANEEAKKILATKENTRVLLTGFWQQGNNFRLSMKKVDGGLLVQEHD
                     SLSLESCELQTVTQIKPTDEQLQNLMFAWLAAKHVKSNAIVYANDLTTIGIGGGQTSR
                     VMSARIGLWQAEQMGFDPKGAVMASDAFIPFPDTIEIAAKAGISAIIQPGGSIRDEKI
                     ISCADQHNIAMIFTGVRHFKH"
     gene            7092..7877
                     /locus_tag="I9760_001093"
     CDS             7092..7877
                     /locus_tag="I9760_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HAT5042869.1"
                     /translation="MTTEQYPSSLVNRLAITEPALVPSGYYRSKLFIAPFSTNVLVAA
                     AGPILSLLERLCLSPSLPPVEDIRENIQHELRAFHSKLDASKYPLDIISIAQYLLSAT
                     IDEILGKSYLRVYNLTTEFKSFTPLSSDGAQPQQRFFEILNYIKERPNQFLDLIELAY
                     FCLIAGFEGEYHLKADGRQCLDNTIEDLYQIIQKYRFNKPHRLFNENPLPKTIKKNYK
                     ATWITTIAAVGIVILAFVTSHVLLENKAKTVLFGHTQLAMLEN"
     gene            7877..10798
                     /locus_tag="I9760_001094"
     CDS             7877..10798
                     /locus_tag="I9760_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF"
                     /protein_id="HAT5042870.1"
                     /translation="MDNSLRALCDAIKKILSQLKPQTNQLSFVVITGKNAQGKSALLK
                     QSNMEEMPVFSEEHAKLYFNQKGIIVELGENWLTNSKTLLLTTLKQLNKCNRHLKITG
                     LILCVDVNDLLIAEPAQFTEQKKSHLQLIQRLGVNLGYQAELALIFTKMDTLAGFSEF
                     YQMDHVNDLSKPLGFSLDCVNQLSKKIETFSLQFNQLIEALGQQVINKMHPARSTIKR
                     SLIREFPLQLASLRAPIQSLIQGISPKLFHVHSIFFTSAEQGGVSVDRLNKKIQHEYA
                     LVVQDTFPQATNFRSYFVEGALQTIQEQCIQVPQTTKFSQKPFIAVAVSLAGLSLLTL
                     VYNHYKTSHILDEASKELLAYEALNNQGTSGAQALYHLSQAANKMNHITSNSISLPTV
                     HQLKLNLHNNAQNRLQGEFLPSLANELEQVITSPGNTPITRYKALKIYLMLGNSKYFS
                     SQPILDWFQQQWQGRAPGTTQKQLALLKQTLSNPPQNIPIKSQIISDARNYLNALPTS
                     YLYYSIAKEYFPQEKQKIVIEGFSIPTDEVPTYFTKTGFTKIINELPKITSTLQSENW
                     VLARQDLSQLHNMLIQAYCFDYVTWWQTFMKKSQPLRYQDYQQGRQLTKLIQQSNSFY
                     KFANLIQQETKPDLTEGISTFNQFIANQFTDLNLTTLSSIKELDFRISELERFIATLS
                     VVNDGGKTAFTITRSRFLNENSSDPVSLLYSQARQLPEPLSSWTKQIAGDIWVILIRD
                     SRQYINNQWQKTVYREFQNSIAQRYPFDSSQTAEIDIADFNRFFSTHGLLNTYSEQYI
                     KPFLDTSSPEWKPKAVNDFILPISNEVIDELIRANIITNMFFPDHSDESKIDFSLQKI
                     SLDPVVSNLVLEIGGTKLMDNQTSDSLIRFTWPQNNAKLALDSIEGNHYELAEYGTWA
                     IFKLLEKVNVLVDEQDSSSLQILFEVNSNSGRYLLKTNNQINPFTPGILNGFTLNDHI
                     A"
     gene            10801..12057
                     /locus_tag="I9760_001095"
     CDS             10801..12057
                     /locus_tag="I9760_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAT5042871.1"
                     /translation="MKQNTKHSIIAGLYGNALEWYDFLLYASFAPVFAEIFFPSKIHF
                     VSLIATFSLFAIGFIMRPIGGLLLGHYADHVGRRKALILSMSVMTISTALIAVLPDFN
                     SWGIIAPLLFSVLRLIQGLAVGGELPGSTTYLIEHMFQQSRGLAGSLVLSSAFLGIFI
                     GALTASIFSTLFTGDALLNWGWRYAYLLGGILGILGIYLRIKSFESPTFLKDKHTAEL
                     PAKVVFTRFKQQLFLSVIITSILAMSNYVLIAYVTTFLVKSEYFLLKDALLINLIALA
                     LLTVLIPLMGFLSDVVGRKPIFLIGLFALCLLIFPFFWLLLSGSWWKALCSELLLAIA
                     LAPINATVPTIIAEMFPTSVRASGISISYNIGQALFGGTMPLVAFSLIELSSNKMAPA
                     WYLFLWTLIVIPITISIRESYQKELT"
     gene            complement(12203..15232)
                     /locus_tag="I9760_001096"
     CDS             complement(12203..15232)
                     /locus_tag="I9760_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmB"
                     /protein_id="HAT5042872.1"
                     /translation="MANWSESFFEGVDTFFAWLSTSLKQTTESYIDLETADSPTVLVN
                     HDGSLLSVLKIEGITGLAGPEEFERLVEGLANSFQAAMGRPGHALQVYFSHDKQNIVK
                     MIKDIYDPAEATASRLELNLRDLFEERVNYLSQYCAEERVYFVLYTRPFNLASDQLKA
                     ANKAKMKMIKDTKAPPFKNTQTIFAAVPEIRDTHDAYVRAILNDLDALNVIAKLLEVH
                     DAVHAIRMTGDPDYTADDWRATLPGDKIPVRELNNFEGDPSDLLWPPLSRQVLPRDAE
                     IIDLRTVRVGDKIYASTYIDLFPKDVRPFISLFSRILPSHVPWKISFLLESEGLSTIK
                     LKGLLAAILSFSSAQNRLISDSVNLLKYIQLNTDEAIVRLRVVATTWAPEGNIPLLRR
                     RSSELVKAIEGWGSTDVSEICGDPFAGFVSSMLATTLNSSAVASVAPLSDVISMLPIT
                     RPASPWKTGALLFRTPDGKPWPFQPGSTEQTTWIDLVYARPGSGKSVLSNALNLALCL
                     SGGLMRLPRIAIIDIGPSSSGLISLLKEALPASKRHLVAYHRLRMTPDYSINPFDTQL
                     GCRYPTALERSFLVNFMTLLTTPLGAAKPYDGMADLAGMVVDELYKSMADEFNPTPYA
                     PGIEEFIDGILEEIGFVRDAKSTWWEVTDALYSAGFVHEAMLAQRYAMPLLADAASIC
                     RTPSIEDLYEKITAPTGESLINAFSRMISSAVREYPILSRVTSFDIGDARVVSLDLDE
                     VAKSGGDAADRQTAVMYMLARYVLARHYYLTEESLNNVPEQYKEYHKQRVLEIREDHK
                     RIVYDEFHRTAKSSAVREQVIIDMREGRKWKVQISLLSQAVDDFDPVMIDFATAIYVM
                     DAGPSQAVEKTSQIFGLSETAKIALRTRVHGPRQGGATFLAQFATKTGINVQLLTLTL
                     GPVELWAFSTTAEDATVRNLLYRHLGPSEARRLLAALFPNGTVTKELEARLASMKQKV
                     GLIEDDEREGMINQLVNDILDAYSKDPNVKSLPAKVG"
     gene            complement(15268..15894)
                     /locus_tag="I9760_001097"
     CDS             complement(15268..15894)
                     /locus_tag="I9760_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAT5042873.1"
                     /translation="MADNQQRCELKLIASPGSWRLYSARKIDERFKSYEQKIFQRDRY
                     TCQFCGFQARLYQDIVNLDGDYTNNRLSNLVTACCFCAQCFFVESVGVGGYGGGTLIY
                     LPELTQAELNSLCHVLFCAITNDTGYKSSAQNIYRSFKFRSQIVEEKFGEGTSDPAIF
                     GQLMIDSGVNSEEIREKLFKNIRLLPSRAKFRKQIEKWAASALEEIAD"
     gene            complement(16092..16490)
                     /locus_tag="I9760_001098"
     CDS             complement(16092..16490)
                     /locus_tag="I9760_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmD"
                     /protein_id="HAT5042874.1"
                     /translation="MNIKVIHKSGYKHWLVSAACLALLTLICQDAAAGVSLGKMASQI
                     TGSFTSLTKLITAGSYLAGLGFSIGAIMKFKQHKDNPTQIPIGTPIALIFIAAALLFL
                     PSILGVAGVTMFGGSGGKTAGPTGTIYKTN"
     gene            complement(16516..17100)
                     /locus_tag="I9760_001099"
     CDS             complement(16516..17100)
                     /locus_tag="I9760_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmC"
                     /protein_id="HAT5042875.1"
                     /translation="MADPTCTGGTVACWIVSQANILANIANQLEPVQRLITGGAYLIG
                     CAFIFKAIYSLKVYGEARTMMSSNTSIKEPVMYLMVGALLIYFPSLVSSVLQTTFGYS
                     NPLAYSGGVSSGSDTISALFGSGSLVGRPLVMIIRVIGLIAFVRGWVLIARSASQGQP
                     PGGTGKGLIHVFGGILAINIVGTVDMINNTLYGT"
     gene            complement(17108..17917)
                     /locus_tag="I9760_001100"
     CDS             complement(17108..17917)
                     /locus_tag="I9760_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmG"
                     /protein_id="HAT5042876.1"
                     /translation="MMAEHDQNNDEYKFAELDSYDMDQAGESDLDSEASYQSGKEGLT
                     KKKDIKRNALIAIGAVVFIMVMYKIIGWMFFSDKSSQVTSKPAIPPVTQVATPQPVQT
                     IPTTTPIQQVQPTTIIEDDPDLKKKVSAIEMTQQSLRSEVNALSEQINAVNNNIKNLN
                     AQIVNLNQIIGNMSNQIARQSEVINVLMARTTPKKVVKVSRPIVQARIIYYIQAVIPG
                     RAWLIGSNGSTLTVREGSKIPGYGMVKLIDSLQGRILTSSGQVIKFSQEDS"
     gene            complement(17932..21078)
                     /gene="dotG"
                     /locus_tag="I9760_001101"
     CDS             complement(17932..21078)
                     /gene="dotG"
                     /locus_tag="I9760_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotG/IcmE"
                     /protein_id="HAT5042877.1"
                     /translation="MASKKENLKSLFSNTRTRVIIIFTAALLIIAVVIGFFKIRGATT
                     GSIAAAEVSTVPGGIQSIPGVLDPTAQYAKLQEEQNITQAQVAEKTGGSAIPTIIRTQ
                     ALGEGVGVIGSQSGVGFAALAQEELGGPQRSLWIQELQDGSCSKSVITKVVNQGAQLT
                     DLKAACSCVQLKDSGYGLQELEQVCECKELKSAGYNARQLKEAGYSAGRLRNCGFDAC
                     ELRNAGFTAQEMKDGGFSDGELKGAGFSDAEIAKASGLPDGITADDVRKAGCGAAALA
                     KLRQAGVSASAIRKISGCTAEQLKAAGYTAKELKDAGFSAADLRRAGFSAAELKDAGF
                     TARDLLNAGFTPADLAKAGFSDAQIKAAQAELPPGITPQDVKNAGCDVEALKKEREAG
                     VSAALIRQYAGCSAQALKAAGFTDADLANAGFTPAQISAATPLSDAEIKAAGCDPDKL
                     KKLFSAGVSAKRIKELNGCSAEALKAAGYDAQSLLAAGFTPQELLAAGFTPKQLEDAG
                     LNPVSIIADGRVADCSVESLKKARAAGVSALTIKQTLGCSAAALKAAGYTAKELKDAG
                     FTAAELKAAGFSAKELKDAGFTAKELRDAGFSAQELKDVGFSAKDLKDAGFSAAELKA
                     AGFTAAQLKAAGFSAKDLKDAGFSAAELKAAGFSAKELKDAGFSASDLKNAGFSAKEL
                     KDAGFSASDLKSAGFSASELKNAGYSADELKKAGYTSAELRNAGFSPQESAVAGLQGP
                     DLQQLDSSITGIPSIPGATPRPTTSDAASSAEQLQAILQKQNEQLAEQKYQQEIQQRT
                     SDMLTAATQLVQDWKQVETQVYTEGTEETKTSGGESAVPGTGTGTGSNNQPVDQGAVS
                     AQNQAIIKTGDIMFAVLDTSVNSDEPGPILATIVTGKLKGSKLIGSFNLPSNADKMVI
                     TFNTMSIPGAEKTISISAYAIDPNTARTALASRTNHHYLMRYGSLFASSFLQGFGNAF
                     QSANTTITIGGTGGGNNITVANGVGRSTLENAVIGLATVGKAWSQQAQQLFNTPTTVE
                     VYSGTGLGILFTQDVTTI"
     gene            complement(21083..22165)
                     /locus_tag="I9760_001102"
     CDS             complement(21083..22165)
                     /locus_tag="I9760_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmK"
                     /protein_id="HAT5042878.1"
                     /translation="MKKYDQLCKYCLVIGLTFSMSCSIYAADQSDDAQQALQQLRMLQ
                     QKLSQNPSPDAQSGAGDGGDNAASDSTQQPNQSGQANAPAANQTATAGGDGQIISQDD
                     AEVIDKKAFKDMTRNLYPLNPEQVVKLKQIYETSEYAKAATPGTPPKPTATSQFVNLS
                     PGSTPPVIRLSQGFVSSLVFLDSTGAPWPIAAYDLGDPSSFNIQWDKTSNTLMIQATK
                     LYNYGNLAVRLRGLNTPVMLTLIPGQKAVDYRVDLRVQGYGPNAKSMPTEEGIPPSAN
                     DLLLHVLEGVPPPGSRRLVVSGGDARAWLSNEKMYVRTNLTILSPGWLASMTSADGTH
                     AYEMQKSPVLLVSWHGKVMQLKVEGL"
     gene            complement(22168..>22443)
                     /locus_tag="I9760_001103"
     CDS             complement(22168..>22443)
                     /locus_tag="I9760_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein DotI"
                     /protein_id="HAT5042879.1"
                     /translation="QSNNLDAVKAKKLVVSAVATRAPIILQKGVLNGRYSWRVQMPIL
                     VTYQSASEFTQQNNVVTMLITRVSTLNSPRGIGISQFVVGPASGGVS"
BASE COUNT         6767 a         4889 c         4044 g         6743 t
ORIGIN      
        1 taaaaacaag tagtgaaaat aatacactta actacccgtt aactcacctc cactctgatt
       61 aatacttgcc ctggagtcac tttgtctccc ttttgacata atatttctgc aaccacccca
      121 tttgcaggtg cttttatttc tgtctccatt ttcattgcct caataaccag tacagcctgc
      181 ccggccttga cttcatcacc aacagaaaca tgaattgcaa taatagaacc ggggatagct
      241 acagtaatat ctccaggtcc tatcttgtta ttaacagatg aacgctctat tttgtcatga
      301 agttcagaat gttgcacaac aacttcttca ggaacaccat caacccacaa aaaacaggat
      361 tgttgaccat gctcaatcat tccataacca gctactttta catgataact ctcaccatga
      421 agaataatat caaactcaga cataacggaa ttatctggtg cacttgattg agtcaataat
      481 ggctcaggaa ttaattgatt atttttacgc tgctctaaga attggcgccc aatttcggga
      541 aacatcgcat aaagaagaac atcctcatca cttagtgcca aatcacttat ttcattttgc
      601 aattgatcta attcattagg taacaaatcg ccaggcctga cttcaatcac ttctgttcgt
      661 ccgatcgctt ttttccgtaa agcagaacta attttgccag gaggagtgcc atattttccc
      721 tgacagtaga gtttcacttc attggttatt gttttatacc gttctcccgt tagaacatta
      781 ataactgcct gagttccaac gacttgagat gttggcgtaa ccaaaggcgg ataacccaag
      841 tccttacgaa cgcgggggat ttccttgtga acagcatcca tcttgtctaa tgcattctgc
      901 tcccttaact gattatacag atttgagatc atgccacctg gcacttgata caattgaaca
      961 cgagggtcaa tattctgtgc ctcactttca aattgagaat acttcttgcg gacagcttta
     1021 aaataatcgt ctatttccaa taaaatattt aaatccaatt ccgtgtcata cggagtgtca
     1081 gtcaatgcgg caaccaatgc ttccgtaggt ggatgagatg caccgccaga aaaagatgaa
     1141 atcgctgtat caatgtgatt acaacccgct aaaacggcct cataatggca tatacttgcc
     1201 aaaccggaag tagaatgact atgcaaatgt acaggtaagc cagtcgcttg ttttaaaccg
     1261 gcatacaatt cgacagtcac cgttggggtt agcaaacctg ccatatcttt tatggcgata
     1321 ctatcacatc ccatttcagc caattttttc cctagctcca aaaaattgtc caaagtatga
     1381 accgggctgg ttgtgtaaca aattgctccc tgggcatgtt ttttatgtga tttaatcgcg
     1441 tctatggcta ctttaaggtt tcgcgcgtca tttaaagcat caaacacacg aaatacatcc
     1501 actccattat tcacggccaa tttaacaaac gctcggacta catcatctgc ataattccta
     1561 tagcctaaca aattttgacc tcttaacagc atagagagtt gagtattcgg taacatttgg
     1621 cgaagttgac gcaatcttga ccagggatct tcctttaaaa atcgcaaaca ggcatcaaaa
     1681 gtagcgcctc cccagacttc catagcccaa aatcctacat catccatttt attacagatt
     1741 ggcaacatat cttcagtacg cattcttgta gcgattaagc attgatgggc atcacgcaag
     1801 gtgacatctg ttataaacgt tttagacata agctcttcct ggctcaagaa ataaataaaa
     1861 atatcataaa cctaaaatcg gtacactctt taagtacaat ccattgcttt ttactaaata
     1921 taaaatttct tgctaaactt gcggagatct tgccgatatc ctcgcagata tatagacatg
     1981 ttcataaagt tcaggcaaaa tctgcaaatc tcagactata ctgatgtgta atgaaaaaaa
     2041 ttctagtatt acatggtcct aatctaaacc ttttaggctc ccgagaaccg tccatttatg
     2101 gacatgcttc gttaactcaa atcaatggtg atttaatcca ggaagcagac aatgcaggga
     2161 ttcgactcag ctgctttcaa agcaatgctg aagcagagtt aattcaagct gtccatcagg
     2221 ctggaattga taaaatcaat tacattatca taaatcctgc ggcatttact cacacaagca
     2281 ttgctcttag agatgcttta agtgcggttg ccatcccatt tattgaggtt catttaagta
     2341 acattttttc ccgtgaaact tttcgtcacc attcctattt ctcagatatt gccgttggtg
     2401 tcattagcgg tttggggacg aaagggtatt tactggcctt acaagcgatt atcaaagaat
     2461 taaagtagag agaaaaaaca aatggatatc agaaaaatca gaaaacttat tgaactcctg
     2521 gaagaaactg gtatttcaga gattgaaatc aaagaaggag aagagtcgtt aagactaagt
     2581 cggcatagta atatagtagc agaaacagct ccacaaattc attatgtggc tccttctgct
     2641 gtgaagcaaa cggcctctgc tccttcctcc aatacttcta ccaccgagtc tcatgttatt
     2701 gaaaataaac aagttacttc agcctcctca ggccataaaa ttcgttcacc aatggttgga
     2761 acaatgtaca cagcgccatc tccagaaagc ccaccttttg taacagtggg acaatctgtt
     2821 aaagcaggag atactctatg cattattgaa gcaatgaaaa tgttcaatga aattgaagcc
     2881 gatcgtgctg ggaaaattgt tgaaatcttg gtaaaaaacg gggatcctgt tgaatatgac
     2941 caggccttgt ttattataga atagagaggc ctgtttatgc tcagtaaaat agttattgct
     3001 aaccgaggcg aaattgcgct tcgtatactg cgtgcttgta aagaacttgg cattcagact
     3061 gttgctgttc actctgatgt agacaaagat ttattgcacg ttcgcctggc tgatgaaacc
     3121 gtatgcatag gccctgcacc tgcccaaaaa agctatctca atattcctgc aattatatct
     3181 gcggcagaaa ttactgatgc agtagcgata catccaggtt atggattttt atcagaaaat
     3241 gccgattttg cagatattgt tgagcaaagc ggtttccgtt ttattggccc acgcggcgac
     3301 tccattcgtc ttatggggga taaagtttcc gcgattgctg ccatgaaagc ggcaggagta
     3361 ccctgtgttc ccggttcaga cggtccttta ggagatgacg acaacaaaaa tctcgagttg
     3421 gcgcgaaaaa ttggttatcc aatcattatc aaagcagccg gcggcggtgg tggacgtggc
     3481 atgagagtcg ttcatagcga atccaattta cttagtgcta ttgcgttaac ccgaagcgaa
     3541 gcaaaagccg ctttcaataa cccgatagtt tacatggaaa aatttctgga aaatcctcgc
     3601 catgttgaat tccaagtcct tggtgatggg aaagggcatg ctatacattt gggtgagcgt
     3661 gattgctcga tgcaaagacg acatcaaaaa gtcgtagaag aagctccggc tccaggaatc
     3721 actcctgaat taagagaaga aattggcgcg tcagtgatta aagcttgcaa agagttacaa
     3781 taccgaggtg ctggaacctt tgaattttta tttcaagatg gttgttttta tttcatcgaa
     3841 atgaatacgc gcattcaggt tgagcatcca gtaactgaaa tgatcactgg gattgattta
     3901 attaaggaac aaatcaaaat tgccagcgat attcctctga cattaaccca aaaagatatt
     3961 aaattacgcg gccatgctat tgaatgccga atcaatgcgg aagatccaag gaattttatg
     4021 ccctctcctg gaaccataaa attacttcat caaccaggag gcccaggcat tcgttttgat
     4081 tctcacatct acagcagcta tgttgttcca cctaattacg attccatgat aggaaaatta
     4141 ataagttatg gggcaacaag agcagaagcg attgccagga tgcgtaatgc ccttgatgaa
     4201 attatcattg atggcataaa gacaaatatt gaattacacc aacgtattct tcatgacaaa
     4261 tcttttatgg aaggtggcac aaacattcac tatctagaaa aaatgctcaa ggattcatag
     4321 cgtgtggttt caactaaaaa tagaacattg tcctaatgac aagattgaag aaataaccga
     4381 ggaactcgaa gaatgcggtg ccttgtccat cacactcaca gacaaaaatg acaaccccgt
     4441 tcttgaacca gagccaggga caacgccttt atggcctgaa gtaatcattc atgccctatt
     4501 tgcccaagca gaagaggcgc aatacgcaag agaacaattg gttgctaaaa gaccatctct
     4561 gcattgcagt ttggaactgc tcgctgataa aaattgggaa agagcctgga tggatgattt
     4621 tcgacctcaa cgttttggta accgcttatg ggtctgtcct acttggctcc ctcccccgga
     4681 acctgatgcg gttaatttaa tattagatcc tgggttagca tttggtacag gcacacatgc
     4741 gacaacctcc ctatgcctaa cttggctgga gcaggctgat ttaaaaaata aatcaataat
     4801 agattatggt tgtggttcag gaatactctc actcgcggca atcaagctag gagctaaaca
     4861 tgtctatgct gttgacatag ataatcaagc cttacaagcc acacaaagta atgcgcatgc
     4921 caaccatatc actgagagcc aactctctat cagcttcccg gaagcattgc aaaatcctgt
     4981 gcatttagtt atcgcaaata ttttgttggc tccattaatc agtttaaagg aacgttttca
     5041 tcaactgctc ccgtcaggcg cgcatttggt aacatcagga attctggaag aacaggcccc
     5101 tttgctgatt gatgcttatg attccgcatt cacccatatt gcaactgaat attgtgaggg
     5161 atggtcgttg ctggttttta caagcaaata ataactatat cactaaggta tatgtaaatg
     5221 gcgaaaaaac aaatttactc ttctttcaag ccacgacgag cactactgag tgtttccgat
     5281 aaaagaggaa taagagaact cggtcaagtc ttacatgacc aaggagtgga attgatagcc
     5341 actggaaaca cagctgccat attaagagaa cataaattac ccgttaccga tgtcagtgag
     5401 tgcacaggat ttcccgaaat aatggatgga agagtcaaaa cccttcatcc agccattcat
     5461 gctggtttat tagcccgcgg agaagaagac agcccagttt taagacaaca tggaataaaa
     5521 cctattgatt tactgattgt caatttatac ccctttgaac aagtcattaa ccacccggac
     5581 tgcgatttta ataaggccat tgaaaacatt gacattggtg gaccagcaat ggtgcgagcg
     5641 gcagctaaaa atcatgccca tacttatgtc atagttgatc caaatgatta ctcaaaatta
     5701 attcattatt tacaagatca gaaagcacct tcccattgga attttgcgtt agctaaaaaa
     5761 gcatttgccc atactgcagc ctacgatgcc gcaattgcaa attatttaac tactttggat
     5821 aatgattatg tcccaactgg atttcccgac atactaacct gccaatttaa taaaattact
     5881 gatcttcgtt atggtgaaaa cccccatcag caagctattt tttatgctga taaaaattca
     5941 catccaggct ctctaagtac agcaacctta ttacagggaa aacaattatc ctataataat
     6001 attcttgatg ccgatgcagc tctcgattgt gtgaaatcat tttccaatga aaagtcggtt
     6061 tgcgtcattg taaaacatac caacccctgt ggcattgcgc tatccgacac atcacttgat
     6121 gcttacttaa aagcctttca aagtgatccg atatccgctt atggtggaat tattgctttc
     6181 aatggaactc tggatagcga tacggcaaaa gcaatattag aaaaacaatt tgttgaagtg
     6241 attattgcac ctgatgccaa tgaagaagca aaaaaaattc tggcaaccaa agaaaacact
     6301 cgtgttcttc taaccggttt ttggcaacaa ggtaataatt ttagattaag tatgaagaaa
     6361 gttgatggtg gtctattagt acaagaacac gattctcttt ctctggaatc atgtgagcta
     6421 caaaccgtta cccagataaa acccactgat gaacaactac aaaatttaat gttcgcttgg
     6481 ttagcagcca agcatgtcaa atctaatgcc attgtatacg ctaatgattt gaccactata
     6541 gggattggcg gaggacaaac cagtcgagta atgagtgctc gtattggttt gtggcaagca
     6601 gagcaaatgg ggtttgatcc caaaggcgct gttatggcct cagacgcatt tatccctttc
     6661 cctgatacca ttgaaatagc agcgaaggct ggtatatccg caattattca accagggggc
     6721 tctatcagag atgaaaaaat catttcttgc gccgaccaac acaacatagc gatgattttt
     6781 acaggagtgc gacattttaa acattaatgt caagcatgta ttaattccaa gcttgactaa
     6841 cttgaacaaa gagtcgtata taacgtatca tcttcttttt aattgaggct ttttgattag
     6901 cataaatgga ccttttcttg taggactgat ggacttgata ttagaacaag gagcaagtat
     6961 ttctagaagc agattttacc tatagaataa ataagaatta taggaatact tactaatcaa
     7021 ctagcaatca cgcaaaagac aaaaatgcta ctgaaagtct aatgcatcac tgaaacccat
     7081 ggagtaatat aatgacaact gagcaatacc cgtcctcact tgtcaatcgt cttgcaatca
     7141 cagagcctgc gttggttcca agtggatatt atcgttcgaa attatttatt gccccatttt
     7201 ctacaaatgt tttagttgca gcggccggac caatactgtc tttattggaa agactttgtt
     7261 taagtccttc tttacctcca gtagaggata taagagaaaa tatacagcat gaattacgcg
     7321 cctttcacag taaattagac gcttctaaat atccgttgga tattattagc attgctcaat
     7381 atttattatc agctaccatt gatgaaatat taggtaaaag ttatctaagg gtctataacc
     7441 tgacgacaga gttcaaatca tttactccct tatcaagtga tggggcacaa ccccagcagc
     7501 gtttttttga aatattgaac tacattaaag agaggccaaa tcaatttctc gatttaattg
     7561 aattggccta cttttgcctc attgccggat ttgagggtga gtaccatctg aaagcagacg
     7621 ggcgacaatg tcttgataat actattgaag atttatatca aatcatacaa aaataccgtt
     7681 ttaataaacc acatcgcttg tttaatgaga atcctcttcc caaaactatt aaaaaaaatt
     7741 ataaagcaac gtggataaca actattgctg ctgtaggtat tgtaattcta gcttttgtta
     7801 ctagccatgt tttattagaa aacaaagcta aaactgtttt atttggacat acccaacttg
     7861 ctatgctgga aaactaatgg acaattcatt acgcgcatta tgtgatgcta taaaaaaaat
     7921 tctctctcaa ttaaaaccac aaactaatca attatctttt gttgtcatca ccggaaaaaa
     7981 cgcccaaggc aaatcggcac tgttaaagca aagtaatatg gaagaaatgc ccgtattcag
     8041 cgaagagcat gccaaactgt attttaatca aaagggaatc atagtagagc ttggtgaaaa
     8101 ttggcttacc aatagcaaaa cattattgct cacaacactt aaacaattaa ataaatgcaa
     8161 tcgccatctc aaaattacag gcttgatttt atgtgtggat gtcaatgatt tactgatcgc
     8221 cgaaccagca caattcactg aacaaaaaaa atctcatttg caacttattc aacgtttagg
     8281 tgttaacctg ggatatcagg ctgaattagc tttaatattc accaaaatgg ataccttggc
     8341 tggcttttct gagttttatc aaatggatca tgtcaatgat ctatctaaac ctcttggctt
     8401 ttcccttgat tgtgtcaacc aattaagtaa aaaaattgaa acgttttccc ttcaattcaa
     8461 tcaattaatt gaagctttag gacagcaagt tatcaataaa atgcacccgg cacgttctac
     8521 tattaagcgc agtttgatac gcgaatttcc tttgcaatta gccagcttga gagccccaat
     8581 tcagtcgtta attcagggga tttcgccaaa attattccat gttcactcta tttttttcac
     8641 gagtgccgag caaggtggag tcagtgtaga taggctaaac aaaaaaattc agcatgaata
     8701 cgctctggtt gttcaggata ctttccctca agcgaccaat ttcagatcct attttgtaga
     8761 aggtgcactg caaacaatcc aggaacaatg catccaggta ccacagacta caaaattttc
     8821 tcaaaaacca ttcattgctg ttgcagtaag tttggccggg ctcagtttat taacgctggt
     8881 ctataaccac tacaaaacct ctcatattct tgatgaggct agtaaagaat tgcttgccta
     8941 tgaagcactg aataatcagg gcactagtgg tgcacaagcg ctatatcact tatcgcaggc
     9001 tgcaaataaa atgaatcaca ttacatcaaa ctccatatcg ctgcctactg tgcaccaatt
     9061 aaaattaaac cttcacaaca atgctcaaaa tcgtttacaa ggagaatttc ttccctcctt
     9121 agctaatgag ttagaacaag tcatcacaag tcctggcaac acccctatca cccgttataa
     9181 ggctttaaaa atttatttaa tgttaggaaa ctcaaaatat ttttcatccc aacccattct
     9241 ggattggttt caacaacaat ggcagggacg tgcaccaggc actacgcaaa aacagttggc
     9301 tcttttaaaa caaactctta gcaatcctcc tcaaaatata cccatcaaat cacaaataat
     9361 cagtgatgcg aggaattatc ttaatgcact tccaacaagc tatttatatt actctattgc
     9421 gaaagaatat ttccctcagg aaaaacaaaa gattgtgatt gaaggttttt ctatacctac
     9481 ggatgaagtg ccaacctatt ttactaaaac cggatttaca aaaattatta acgaattacc
     9541 taaaattacc agtacacttc aatcagaaaa ctgggtactt gcgagacagg atttgtctca
     9601 attacacaat atgctgattc aggcttattg ttttgattac gtcacttggt ggcaaacgtt
     9661 catgaagaaa tcccaaccat tacgttatca ggattatcag caaggtcgtc aattaacaaa
     9721 attaatacaa caatccaatt cgttttataa attcgctaat cttattcagc aggaaaccaa
     9781 accggattta accgaaggca tatccacctt taatcaattc attgccaatc aatttactga
     9841 tttaaattta acaactttat ccagcattaa ggaattagat tttagaattt ctgaattaga
     9901 acgatttatt gcaactttat ctgttgtaaa tgatggaggt aaaacagcgt ttaccattac
     9961 acgctcgcgt tttctcaatg aaaattcctc tgaccctgtg agtttgctct acagccaggc
    10021 acgacaatta cctgaaccat tgagttcgtg gacaaagcaa atagctggtg acatttgggt
    10081 tatactgatt agagatagtc gtcaatacat taataatcag tggcaaaaaa ccgtctatag
    10141 agagttccaa aactctattg cccagcgcta tccttttgac tcgtcacaaa ctgcagaaat
    10201 agatattgca gattttaatc gctttttctc aacacatggc ttattgaaca cctactctga
    10261 acaatacatt aagccatttc ttgacacttc atctccagaa tggaagccta aagctgtgaa
    10321 tgatttcatc ttgcctattt caaatgaagt aattgatgag ttaattcgcg ccaatatcat
    10381 tactaatatg ttctttcctg atcacagtga tgaaagcaaa attgatttta gcttgcaaaa
    10441 gatctctctg gatcctgtag tttctaatct agtccttgaa attggcggca ctaaattgat
    10501 ggataatcaa acaagcgatt cactaattcg cttcacctgg cctcaaaaca acgctaaatt
    10561 ggctcttgat tctattgaag ggaatcatta cgagctcgca gaatatggaa cctgggcaat
    10621 atttaaatta cttgaaaaag tcaatgtgct tgtggatgaa caagatagtt caagcctcca
    10681 aatacttttt gaagtgaata gcaactccgg ccgctattta ttaaaaacaa acaaccaaat
    10741 taatcctttt actccaggaa ttctaaatgg gtttacttta aacgaccata ttgcataatt
    10801 atgaaacaaa ataccaaaca cagcattatt gctggattat acggaaatgc tttggagtgg
    10861 tatgatttct tgctctatgc cagttttgct cctgtctttg ctgaaatttt tttcccttca
    10921 aaaatccatt ttgtttctct tatagcaaca tttagcttat ttgctatagg attcattatg
    10981 cgcccaattg gtgggctttt gctcgggcac tatgctgatc atgtaggacg gcggaaagca
    11041 ttaatactat ctatgtcagt aatgaccatt tctaccgcat taattgcagt attacctgat
    11101 tttaattcat ggggaattat cgccccactt ttattttctg ttttacggct cattcagggg
    11161 cttgcagtgg gaggcgaact gcccggctcg accacctatt tgattgagca tatgtttcaa
    11221 caaagccggg ggctcgctgg cagcctcgtg ttaagtagcg cttttttagg tatttttatt
    11281 ggagcgctta ccgcctcaat attcagcaca ttatttacag gtgatgctct attaaattgg
    11341 gggtggaggt atgcgtatct gttaggaggt attttaggaa ttttgggcat ttatttgcgt
    11401 attaaaagct ttgaatcacc cacattccta aaggataagc atactgctga acttcctgca
    11461 aaagtggtat tcacgcgatt taaacagcaa ctgtttttat ctgtgataat tactagcatt
    11521 cttgccatgt ctaattatgt acttatcgcc tatgtcacaa cttttttagt caaatcagag
    11581 tattttctct tgaaagatgc gttattaatc aatttaattg cccttgctct tttgacagtt
    11641 ttaataccgc tgatgggatt tctttctgat gttgtgggac gcaagcctat tttcttaatc
    11701 ggtttatttg ctctttgcct actaatcttc ccattttttt ggctcttatt aagtggtagt
    11761 tggtggaaag cattatgcag cgaattatta ctagcgatag ctttagcccc aatcaatgcg
    11821 acagtaccca ctattattgc agaaatgttt ccaaccagtg tacgggcgag tgggatttcc
    11881 atatcctata acattggcca ggctttattt ggcggtacca tgcctttggt tgcgtttagc
    11941 ctgattgaat taagcagcaa taaaatggct ccggcttggt accttttcct atggaccctt
    12001 atcgtgatac caataactat ttccattcgt gaaagctatc aaaaagagct tacttaaggc
    12061 attagattga gatgaaccga cataatttct atcgattgaa taaacaacaa tagatttaaa
    12121 tcagtttttt agtagaaaaa acccagcatt aatagccaga aaataagtgc attgagccat
    12181 actaataaaa atcttacctg cttcaaccta cttttgcagg caagcttttc acgttgggat
    12241 cttttgaata agcatcaaga atatcattca ccaattggtt aatcattccc tctctctcat
    12301 cgtcttcaat taacccaact ttttgcttca tgcttgctaa tcgagcctcc aattctttag
    12361 tcactgtacc atttgggaac agtgcagcta aaagccgtcg agcttcactt ggtcccagat
    12421 gcctatacaa aagatttcgt actgtggcat cttctgcagt agtactaaat gcccataatt
    12481 cgaccgggcc caatgttaag gttaataatt gtacgttgat acctgtttta gtagcaaact
    12541 gagcaaggaa agtagcgcca ccttgtctag gaccatgaac tcgagtacgt aacgcaatct
    12601 tggctgtttc agaaagtcca aaaatttgac tggtcttctc gacagcttga gatggtcctg
    12661 catccataac ataaatagcg gtagcaaaat caatcattac cggatcaaag tcatctactg
    12721 cttgtgataa taaggaaatt tgcactttcc attttcggcc ttcacgcata tcaataataa
    12781 cttgctcacg aacagcagac gattttgccg tgcgatggaa ttcatcataa acaatacgct
    12841 tatgatcttc tcgaatctct aatactctct gtttgtgata ctctttatat tgttccggta
    12901 cattattcaa actttcttct gttaaataat agtgccttgc caatacatat ctggctagca
    12961 tatacataac cgctgtttgt ctatctgccg catcaccacc acttttcgct acttcatcca
    13021 aatcaagaga cactactctt gcgtcaccaa tatcaaaact agtgacccgt gataaaatag
    13081 gatattcgcg aacagctgat gaaatcattc ttgaaaaagc attaattagt gattcaccag
    13141 ttggcgccgt aatcttttca tataaatcct caattgatgg tgttctacag atagaagcag
    13201 catccgctaa taacggcatt gcatatcttt gcgctaacat cgcctcatga acaaaacctg
    13261 ctgaatagag cgcgtccgtc acttcccacc aggttgattt ggcatctcgg acaaagccta
    13321 tttcctccag gatgccatcg ataaattcct ctattcctgg agcatagggt gtcggattga
    13381 attcatcagc catgctttta tataattcat ctaccaccat acccgccaaa tctgccatac
    13441 cgtcatatgg ctttgcagca cctaatggcg tagttagcaa ggtcatgaag ttgactaaga
    13501 atgatcgttc caaagcagtt ggataacgac atcccaattg ggtatcaaag ggattaatcg
    13561 aataatctgg ggtcatcctt aatctatgat aagcaaccaa atgacgctta gatgcaggga
    13621 gagcctcttt taacaatgaa atcaaaccgc tacttgaggg gcctatatca ataattgcta
    13681 tgcgcggcaa acgcattaaa ccacctgaaa gacaaagagc taaattcagt gcatttgaca
    13741 acacagattt accagaacca ggacgagcgt aaaccagatc aatccaagta gtttgttcag
    13801 ttgagcctgg ttgaaacggc cacggtttac catctggtgt tctaaaaagc aaggctcctg
    13861 ttttccaggg agatgctggt cttgtaatag gcagcattga gataacgtct gacaaaggag
    13921 ctactgatgc tactgctgag ctgttcaaag tagtagctag catacttgac acaaagcctg
    13981 caaacggatc cccacaaatc tctgatacat cagtagaacc ccaaccttca atagccttta
    14041 ctaactcaga acttctccgc cgcaacaagg gaatattgcc ttcaggagcc caggttgttg
    14101 caacgacgcg tagtcttaca atcgcctcat cagtattgag ttgtatatat ttcagcagat
    14161 taaccgaatc actaattaaa cgattctgtg cagagctaaa gctcaatatc gctgccaaga
    14221 gccccttcaa tttaattgtg cttaatcctt cgctttctag caaaaaagaa attttccagg
    14281 gcacatgtga aggaagaata cgagaaaata aactaataaa tggtcttaca tcctttggaa
    14341 acaaatcaat ataggttgaa gcataaattt tatcgcccac tcggacagtg cgtaaatcaa
    14401 taatctctgc atctcttggc aaaacctgcc tggacaacgg cggccataat aaatcagaag
    14461 gatccccctc aaaattattc agctcgcgaa caggtatttt atctccaggt aaagttgcgc
    14521 gccaatcatc tgcagtataa tcaggatcac ctgtcatgcg aatggcatgt actgcatcgt
    14581 gcacctcaag caacttagct atcacattaa gggcatccaa atcatttaga attgcacgca
    14641 cataagcatc atgtgtatct cttatttccg gaacagccgc aaaaatcgtt tgtgtgtttt
    14701 taaatggagg agcttttgta tccttgatca tcttcatttt tgctttgttc gcagctttta
    14761 actgatcgct cgccaagtta aatggtcttg tatacaaaac aaaataaaca cgctcctctg
    14821 cacaatattg tgataaataa ttgacccgct cctcaaataa atctcttaaa ttaagttcaa
    14881 gacgacttgc tgttgcttcc gcagggtcat aaatatcctt gatcattttt actatgtttt
    14941 gcttatcatg gctaaaataa acttgtagcg catgaccagg tctacccata gcagcttgaa
    15001 atgagttggc taaaccttca acgagtcgtt caaactcttc agggcctgcc aatccagtaa
    15061 tcccttcaat ttttaatacc gacagtaatg atccatcatg attaactaat acggtagggc
    15121 tatccgccgt ttccaaatct atataagact ctgtagtttg cttcaaggaa gtacttaacc
    15181 aggcaaagaa agtatcgacg ccttcaaaaa acgattctga ccaatttgcc atcctttgtc
    15241 ctcaattatt aatagtactt ttactgttta atctgcaatt tcttctaaag cactggcagc
    15301 ccatttctct atttgcttcc tgaattttgc cctagagggt aatagcctta tatttttaaa
    15361 aagtttctct cttatttctt cactattcac accagaatca atcattaatt gcccaaatat
    15421 tgcagggtca cttgtgccct caccaaattt ttcttcaaca atttgcgagc gaaatttaaa
    15481 gcttctataa atattctgtg cactggattt gtatccggta tcattggtaa tagcacaaaa
    15541 aagaacatgg cataagctat ttagttcagc ttgggttaat tctggcaggt agatcaaagt
    15601 acctccacca taaccaccaa ctccaactga ctcaacaaaa aagcattgcg cacaaaaaca
    15661 acaagctgta actaaatttg ataacctatt gtttgtataa tcaccatcca gattcacgat
    15721 gtcttggtat aatcgggctt gaaacccgca aaattgacaa gtatacctat ctcgctgaaa
    15781 gattttttgc tcataggatt tgaatcgctc atcaattttt ctggcagaat ataaacgcca
    15841 ggaacctggt gaagcaatta gttttagctc acatcgttgt tgattatccg ccatagtttg
    15901 gttcacattc agtcaatttt caaaaaatta tgtgttctat ccttaactac tgactaagcc
    15961 agcttaaaga tatttgacca tattaataga gacaatcatt taaatttact atttaacaca
    16021 ataaaaagcc tataaattaa attgggtaaa gcggtattct ctttacccaa ataatataaa
    16081 taatctctct attaattagt cttatatatt gttccagtag gtccagcagt tttaccgcca
    16141 ctaccaccga acatagttac tccggccact cctaaaatag aaggaaggaa taataatgcc
    16201 gcagcgataa atatcaaagc tattggtgtt ccaattggaa tttgagtagg gttatccttg
    16261 tgctgcttaa atttcataat agcaccaata gaaaaaccca atccagccaa gtaagagccc
    16321 gctgtaatca atttagtaag acttgtaaat gaacccgtaa tttgtgatgc cattttacct
    16381 aacgatacac ctgctgcagc atcctgacaa attagagtca ataaagccaa acaagctgcg
    16441 cttactagcc agtgtttata acctgattta tggataacct taatgttcac aattctctcc
    16501 tgtttttaaa ttagcttaag taccatataa agtattgtta atcatatcaa ctgtaccaac
    16561 aatattgatt gccaatattc ccccaaatac atgaatcaaa cctttacctg tacctcctgg
    16621 aggttgcccc tgcgacgcgg aacgcgcaat taatacccac cctcttacaa atgcaattag
    16681 accaataacc cggataatca tcaccaaagg tctgcctact agactgccac taccaaataa
    16741 ggctgaaata gtatcgcttc cactactcac acctcctgag taggctaatg gattgctata
    16801 accaaaagtc gtttgcagta cggaggaaac caatgacggg aaatagataa gtaatgcacc
    16861 taccatcaaa tacatcaccg gctctttgat actcgtatta ctcgacatca ttgtcctggc
    16921 ttctccataa acctttaagc tataaattgc cttaaatata aaagcgcacc caatcaaata
    16981 agcgcctcca gtaatcagcc tttgaacagg ctccaactga ttagcaatat tagctaatat
    17041 gttagcttga ctcactatcc agcatgctac tgtgccgcca gtacaagtcg gatcagccat
    17101 atacgcctca actatcttct tgactaaact taatcacttg tccagaactt gttaaaatac
    17161 gaccttgcaa agaatcaatt aatttaacca ttccatatcc tggaattttt gatccctctc
    17221 taactgtaag tgtagaacca tttgagccaa ttagccaagc ccgacctgga attactgctt
    17281 gtatgtaata aataattctt gcttgaacta ttggccgaga cacttttaca actttcttag
    17341 gtgttgtacg tgccattaag acattaatca cttctgattg tctagcaatt tgatttgaca
    17401 tattaccaat aatttgattc agatttacaa tttgagcatt taaatttttg atattattat
    17461 ttactgcatt aatttgctca ctaagggcat tgacttcaga acgaagactc tgctgtgtca
    17521 tttctattgc tgaaactttc ttctttagat cggggtcatc ttcaataata gtcgtaggct
    17581 gaacttgttg tatgggagtg gtggttggta ttgtctgtac aggttgtggt gttgctacct
    17641 gtgtcaccgg aggaatagcg ggctttgagg ttacttggct ggatttgtct gagaaaaaca
    17701 tccaaccaat tattttatac atcaccatta taaatacaac tgccccaata gcaataagag
    17761 catttcgctt aatatctttt ttctttgtta atccctcttt cccagattga tacgaggctt
    17821 ctgaatcgag atccgattca ccagcctgat ccatatcgta tgagtcaagt tctgcaaatt
    17881 tatattcatc attattttga tcgtgctctg ccatcattat ccgccatcaa attaaattgt
    17941 tgtaacatcc tgggtaaata aaatacccaa acctgtacca gaataaactt ccacagttgt
    18001 tggtgtatta aacaattgtt gcgcctgttg actccatgcc tttccaacgg tagccaatcc
    18061 tataaccgca ttttccaaag ttgagcgacc aacaccatta gctacagtaa tattattacc
    18121 accgccagta cccccaatgg tgattgttgt atttgccgac tggaatgcat ttccaaatcc
    18181 ttgtaagaaa gaagaagcaa acagggaacc atatcgcatt aaatagtgat gattagttct
    18241 actggctagg gctgttcttg ccgtgtttgg atcaattgca taagctgaga tggatatagt
    18301 tttttctgct cctggaattg acattgtatt aaaggtaatc accatcttgt ccgcattgga
    18361 cggtaagttg aagcttccta ttaattttga gccttttagc ttaccagtaa caatagtcgc
    18421 caatattgga ccaggttcat cactattcac agaagtatcc agaaccgcaa acataatatc
    18481 acccgttttg ataatagcct gattctgagc actaactgcg ccttgatcta ctggttgatt
    18541 attagagcct gtgcctgtac ctgtgcctgg aacagcactt tcaccaccag atgttttagt
    18601 ttcctctgta ccttctgtat acacctgggt ttcaacttgc ttccaatctt gaaccagctg
    18661 agtcgctgcc gtgagcatgt cagaagttct ttgctgaatt tcttgctgat atttttgctc
    18721 agccaattgc tcattttgct tttgtaaaat agcctgaagt tgctcagcac ttgaggcagc
    18781 atcactggtt gttggtcttg gagtagcgcc aggaattgag ggaattcctg tgatacttga
    18841 gtcgagttgt tgcaaatcag gcccttgtaa ccctgcaacg gctgattctt gtggagaaaa
    18901 tccagcattt ctcagctcag ctgaggtata tccggctttt tttagctcat cagccgaata
    18961 tccagcattc tttaattctg atgcactaaa tcctgcgctt ttcaaatctg aggcgctaaa
    19021 accagcatct ttcaattctt ttgcgctaaa tccggcattt ttcaaatccg aggcgctaaa
    19081 accagcatct ttcaattctt ttgcgctaaa tccggctgct tttaattccg ctgcagaaaa
    19141 accagcatct tttagatctt ttgcagaaaa cccagcagcc tttaactgag cagcagtgaa
    19201 tccagccgct tttaattcag cagcagaaaa accggcgtct tttagatctt ttgcactaaa
    19261 acccacatct ttcaattcct gggctgagaa acctgcatca cgcaattcct ttgctgtaaa
    19321 accggcatct tttaattctt tcgcactaaa acccgctgcc tttaattcag ctgcagtaaa
    19381 cccagcgtct ttcagttctt ttgcagtata accagctgct ttcaatgctg cagcggaaca
    19441 tcctaatgtt tgctttattg tcaaagcgct aacaccagct gctctcgctt tttttaatga
    19501 ctctacgctg caatctgcaa cacgcccgtc tgcaataatg gatacaggat ttaatccagc
    19561 atcttccaac tgctttggag taaatcctgc cgcaagcaat tcttgagggg taaatcctgc
    19621 agcaagtaag gactgtgcgt cataacccgc agcttttaaa gcttctgcac tacaaccatt
    19681 gagttctttg atacgtttcg cagatacgcc tgctgaaaat aattttttca gtttatctgg
    19741 atcacagcca gccgccttta tttccgcatc actcaaagga gttgctgcac tgatttgagc
    19801 tggagtaaat cctgcgtttg ctaaatccgc atcagtgaat ccggcagctt tgagagcctg
    19861 tgcgctacat cctgcatatt gtctaattaa agcggcacta actcctgctt ccctttcctt
    19921 tttcagcgcc tccacatcac aaccagcatt tttcacatct tgcggagtaa tcccaggcgg
    19981 aagctctgcc tgagcagctt tgatttgagc atcagaaaat cctgcttttg caagatcagc
    20041 cggtgtaaat ccagcgttta acaaatctct tgcagtaaat ccagcatctt ttaattctgc
    20101 agcagaaaag cccgctcgtc ttaagtccgc agcgctgaaa cctgcatctt ttaattcttt
    20161 agcggtataa cctgctgctt ttaactgctc agcggtacaa ccgcttattt ttctaatcgc
    20221 agacgcgcta acccctgcct gacgcaattt ggccaatgca gcagctccac atcctgcttt
    20281 gcgcacatca tctgcagtta taccatctgg taaaccactt gcttttgcaa tttcagcatc
    20341 agagaacccg gctcctttta attctccatc agaaaaacct ccatccttca tttcttgtgc
    20401 tgtaaagcct gcattacgta attcacaagc atcaaatcca caatttctta gtcgaccggc
    20461 actataacct gcctccttca actgccttgc attataacca gcagatttta attctttaca
    20521 ctcacagact tgctctagtt cctgcaaacc gtacccacta tcttttaact ggacacagct
    20581 acaagcagct ttcaaatcag ttaattgggc tccctgattc acaacttttg taatcacaga
    20641 tttgctacaa ctgccatctt gtaattcttg tatccataaa cttcgttgag gaccacctaa
    20701 ctcttcttgt gctaaagcag cgaaacctac tccactttga gagccaataa caccaactcc
    20761 ttcccccaat gcttgagtac ggataatcgt tggaatagca ctcccacctg ttttttcagc
    20821 gacttgtgcc tgggttatat tttgttcttc ttgcaatttg gcatattgag cggttggatc
    20881 taaaactcct ggaatagatt gaattcctcc cggcactgta gaaacttcag ctgcagcaat
    20941 cgaaccggta gtagcacccc ttattttaaa aaaacctatt actactgcaa tgattaataa
    21001 agctgctgta aaaatgataa ttactcgagt ccgagtattg gaaaacagtg actttagatt
    21061 ttcttttttg ctcgccattt gcttataacc cttctacctt gagttgcatg acttttccat
    21121 gccaagacac taataaaacc ggagactttt gcatttcgta agcatgcgtt ccatctgcgc
    21181 tagtcatgct ggctaaccag cccggcgaca atatagtgag attcgttcta acatacattt
    21241 tttcattaga taaccaagct cgagcatctc ctccactaac caccagacgc ctacttccag
    21301 gtggaggaac tccttctaac acatgaagaa gcaaatcatt ggctgatgga ggaatgcctt
    21361 cttcggttgg catactttta gcgttagggc cataaccttg aactcgcaaa tccactcgat
    21421 aatccacggc tttctgcccg ggaataaggg taagcattac aggagtattt aatcctctta
    21481 accttactgc tagattgcca tagttatata gcttcgtagc ttgaatcatt aaagtattac
    21541 tggttttatc ccattgaata ttaaatgaag aggggtcgcc caaatcatat gcagcaatag
    21601 gccaaggagc tcctgttgag tccaggaata ctaaagaaga cacaaaacct tgtgataatc
    21661 ttattactgg tggtgttgat cctggagaca aattaacaaa ttgtgatgtc gctgtaggct
    21721 taggtggagt gcctggcgtt gcagctttgg catactcaga agtttcatag atttgtttta
    21781 atttgactac ttgctcagga tttaagggat ataagttgcg tgtcatatcc ttaaatgctt
    21841 tcttatcaat aacttcggca tcatcttgac taattatctg cccatcaccg cccgcagttg
    21901 ctgtttgatt tgcagccgga gcattggcct gaccggattg attaggttgt tgtgttgaat
    21961 cagaagctgc attatcaccg ccatcaccgg ctcctgactg tgcatcaggg gaagggtttt
    22021 gtgataattt ttgttgtagc attcgcagtt gctgcaacgc ttgctgagca tcatctgatt
    22081 gatcagccgc atagatactg catgacatac tgaatgtcaa gcctatcacc aaacaatatt
    22141 tacataattg atcatatttc ttcatcacta ggatacccca ccgctggcag gtccaacaac
    22201 aaattgtgaa atacctatcc ctctaggcga attcaacgtt gaaactctag ttattaacat
    22261 tgttactacg ttattttgtt gtgtaaattc actggcactt tgataggtga ctaatattgg
    22321 catttgtacc ctccaggaat acctaccatt gagcacacct ttttgcaaaa tgattggggc
    22381 tcgggttgct actgctgaca caactaattt tttcgcttta accgcatcaa ggttattgga
    22441 ttg
//
LOCUS       DACTJE010000037        22265 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.037, whole genome shotgun sequence.
ACCESSION   DACTJE010000037 DACTJE010000000
VERSION     DACTJE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22265
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.037"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(212..448)
                     /locus_tag="I9760_001104"
     CDS             complement(212..448)
                     /locus_tag="I9760_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5042880.1"
                     /translation="MSIIVNLDVMMAKRKCRLRDLAEAIGITEANLSILKNGKAKAIR
                     FATLEAICAYLHCQPGDLLEYQSIEDNHLIKDRA"
     gene            complement(535..1074)
                     /locus_tag="I9760_001105"
     CDS             complement(535..1074)
                     /locus_tag="I9760_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAT5042881.1"
                     /translation="MKKIKSVSHLLFLLFKTLAYGLPLVTTYFILFHFESMLNWGGWH
                     SLLSTPVKDPSHFSLLHRFIILLIEALPLSINVLICHQLAKLFRLYEHGYLFEEENIR
                     LIKNISIYLISGELIQLLYQPLITAALTFTNPSGQRIASITLGTANLSTLITAFIILI
                     ASWIIKEAHKLKTDALLTI"
     gene            complement(1202..2197)
                     /gene="hemH"
                     /locus_tag="I9760_001106"
     CDS             complement(1202..2197)
                     /gene="hemH"
                     /locus_tag="I9760_001106"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAT5042882.1"
                     /translation="MRRGLLLLNLGTPNNADIRAVKLYLREFLTDKRVIDLPTIPRYI
                     LVYCLILPFRSPKSAQAYQSIWTEKGSPLLYHSQNLVTKLQSALKDEYKIALGMRYGT
                     PSITTALAELKDCHSLTILPLFPQYSSAATGSAIEKTLSHLANQEIIPSIKIIRDFYQ
                     RPEYIQAQAKIMKPYIKDNFHLLFSYHGIPERHIHKSGCDTLCPQTCTPIYDKNQACY
                     RAQCYQTSLLLAKELQLGTHQYTTAFQSRLGKTPWIKPYTDEIFAELISKGIKNIVVS
                     CPSFVADCLETLEEIGIRAKEQWEKLGGEQFILTPCMNDHPEWIKAIQSIVNEQF"
     gene            complement(2308..2541)
                     /locus_tag="I9760_001107"
     CDS             complement(2308..2541)
                     /locus_tag="I9760_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAT5042883.1"
                     /translation="MARGEVKWFNNAKGWGFIIPEGGGEDIFVHFSSIHGTGYKTLVP
                     GQAVTYDVVNGERGLHASNVVALSENAEQEEIA"
     gene            2778..3209
                     /locus_tag="I9760_001108"
     CDS             2778..3209
                     /locus_tag="I9760_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HAT5042884.1"
                     /translation="MFCIDDRIKSSSFIIGEWPLSTVLLKNDASYPWFILVPRRMDIQ
                     EIHQLEKSDRFLLIEEINQLSLLASDYFKPDKLNIGALGNIVPQLHIHIVIRRKSDPL
                     WPQGIWQASMPSIPYPESELKSLLPTLGDLVGAYDKPQSLC"
     gene            complement(3423..3782)
                     /locus_tag="I9760_001109"
     CDS             complement(3423..3782)
                     /locus_tag="I9760_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAT5042885.1"
                     /translation="MIKKIAFTMYPVQNMDRARQFYEKTLGLEVGKISAEGAWVEYDL
                     PQGGCFAITTLAQGVIPSAHSGGSVAFEVDNLDDLVFQLKSKGVQFKIDLFASPVCRM
                     AIAVDSEGNAFTLHQLT"
     gene            3878..5221
                     /locus_tag="I9760_001110"
     CDS             3878..5221
                     /locus_tag="I9760_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAT5042886.1"
                     /translation="MLKKLMFLLLLGAGLLSCGQSTDQQISNIITPKQFPSSPMDYRP
                     VQELPYLAWWKQFQDPELDRLIDTGLKTNMDIHIAMANLQQAQGELQQVKLSWIPNVQ
                     ILGGYSTNPALGVPGGFYGIWPSYILNIMQLYTQQKRAQYQVQYHQAAIDGLRLTLIG
                     QVAAAYFTLIAQLEQLKLLYKLDRDLKSLIALSRKEINIGLKNDIDLAQLQSNERLIA
                     AQIKPIKHNIVVSQNALRYLINENPGLIKSKNNFAQLDFTRFKPGSLPATVLGNRPDM
                     KMAEYSLKAARAGIFVAYSDFFPVMQLDDFIGEAHLPDSTFEQATDAYVNWTINPNTL
                     GKISVSKGVYNAKLAEYVKTVRLILKEVDNAFSANKRMREQFLSYRHAQEDYQHKYKL
                     QNGLLKSGLMSYKELLESKVYLDTLALSTNQAKLELAMSLVMLYQDLAGGYACSD"
     gene            5361..6398
                     /locus_tag="I9760_001111"
     CDS             5361..6398
                     /locus_tag="I9760_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAT5042887.1"
                     /translation="MKKLMEYGGKIKEYLLRLKPKRWFTLTNVIIFIGFLTAIIYVFS
                     YLIPFTDNAFVVNNVRPVAALTSGYITELYVKNGDAVRKGQKLFTVFKKPYEYTVEQL
                     SADLAGAQAKLAVLKATYERDLKLSGNEQKIYKKLAQDDQKYLKGYAIKSVSLITLQN
                     SQQETKAAKDKWQASLKQLEIDQHQITVQENEIKSIQARLKNAKVNLDLTDVYAQGNG
                     VIQNLFFTIGTPVNINQPLFSLVDQDNIYIQANFNETDLRDVRKGSKVLIFPRMYLGR
                     KIFHGVIDSDYWSANRQLVDDRTQLQNVINENQWILLPQRLPVIIKVTDPDPKYPLRV
                     GASAYVYVEVY"
     gene            6376..7308
                     /locus_tag="I9760_001112"
     CDS             6376..7308
                     /locus_tag="I9760_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042888.1"
                     /translation="MSMSKFIEWIDEVDPYAVQRIALYKSLFIATVMAYVYWVFRPTN
                     FTAFFSPFLLVRFYESPSLTSFKEKEHFLIFIGVVVVLLSTSFYLVYPFRVVFFFFSI
                     IALASVYFYVLKNYLPLRNVTMLIIASGATILSTEPPANWQIVYDIVGSSALSMIAIF
                     ICLRFFPNQYLSVWKRALAKFIQSLELDIEGSFTHRGPKFMQEEMTHLGMLRQYRRLI
                     PKKYMIYTQRISINIRNIQFSLDNLYYEAKNEAFWHGVKENLYTLRCAIKTNTSCGTP
                     KMSIMPESKLQQYVMRCLQQAFSQWNQLCMILQH"
     gene            7287..8174
                     /locus_tag="I9760_001113"
     CDS             7287..8174
                     /locus_tag="I9760_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAT5042889.1"
                     /translation="MHDTSALKKKLDYARFIHLVIMFMVAMLIFLFSTFPEKWWVLIT
                     VLSVSAGIEPGLIIRRAKHRIGGTLLALIILIPLLYLLQLNYRLISILFVLAIVGLSV
                     ATLNTRRYDISVFFITLVVFFLMAQTEEATSPRGPFEMVLNRGICTLFGVFIVLAGDY
                     FLFQAYRYSHRLYFFHQVIVYDFLNDIVRKIDESNTEKINTLLFVEKLRGQFTEHYLP
                     ISISSENLKLDFKTSEHTKKRIDIFQETIWEIRRLVFALCISEFVLHSSTASEQHLQR
                     FKLLINKARTHFIQFEGRY"
     gene            8196..8576
                     /locus_tag="I9760_001114"
     CDS             8196..8576
                     /locus_tag="I9760_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5042890.1"
                     /translation="MSHPHYSVYNEKCPSQNVLEGISDKWSILVISLLSQKIYRFGEL
                     KREIGGISPKMLTQTLLKLERYGFVQRQSYPVLPMKVEYSLTRLGKELSAILVLLTRW
                     TETNMDKIINAEKTFCEQRLGCHA"
     gene            complement(8578..9105)
                     /locus_tag="I9760_001115"
     CDS             complement(8578..9105)
                     /locus_tag="I9760_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042891.1"
                     /translation="MAQELVLDDITAMMARYQKTCRYTYTNQSPIIMHPDLKDLLDYR
                     NHKIITRYNMDYPTAAMQAEEALHELMKFIWLCHKHKTDKLLYPDDKNLDFSCVIHAE
                     MADIDKMWHTFLLFTRDYHEFCDTRLNGIFFHHDPLIGENTPVSEEDYTLELTRYLTY
                     IHDHLGSETLIKWFQ"
     gene            9271..9882
                     /locus_tag="I9760_001116"
     CDS             9271..9882
                     /locus_tag="I9760_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5042892.1"
                     /translation="MNKRNWTAYYNSTKQNTLPRKSLLKAIANFDKEKINLSKSAIDL
                     GCGAGIDVMELLRCGWSVIAIDSQLSAIDSLLSSAQELTCANKLKTIISSFEALDSLP
                     KVNLINASFSLPFLKPVYFYKFWSVILKGLKPGGRFSGSFFGFNDFWNTRTDMTFLNR
                     EMICHLFSQFKIEYFQEEEKDEMDALGYYKHWHKFFIVAKKIA"
     gene            complement(10049..10858)
                     /gene="ankJ"
                     /locus_tag="I9760_001117"
     CDS             complement(10049..10858)
                     /gene="ankJ"
                     /locus_tag="I9760_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkJ/LegA11"
                     /protein_id="HAT5042893.1"
                     /translation="MIKMGRSEMKIASAELRELMKAVSEGHYETVNTILDKDPELVNQ
                     YAPPTYDSPLARVLNKKHIDYKMLDILVKHHVDFDYPINYHKETPIELACKNQDLQLF
                     KYLVQHNAPISEQAPHFLLVNSTNIKYLTEDKIKNTCEIIKLMGGLEAVSSKCDAEGN
                     RFGEQARKSQLINRFGGIVKYDYMQLLQSVYPIVDREVDAPTIHGSTEVLTNLLNKIR
                     GQFSSKETYDQQNLKDSISLFFMTGGEIPPSRKVPESRFEEAGIDTPKNAL"
     gene            11121..13121
                     /gene="ceg14"
                     /locus_tag="I9760_001118"
     CDS             11121..13121
                     /gene="ceg14"
                     /locus_tag="I9760_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg14/sidL"
                     /protein_id="HAT5042894.1"
                     /translation="MQNLDEILKKLRKDLKKTSKETTEKLSVLSKKVAEDVVKVSNQT
                     VQKINEVTKKFTVDTTVSTIDTQKILDDFMKNIRFSRFKQIWQAIEKTPKSKHILLKS
                     LFLKGLLTHNKPLLEEIIRQIELIPYSSDLEMLGAFSQDKAHPLSPELLEFVQEMLAN
                     ESEKVLLTILMNAFQSIPELSLDSKTGTLSMKTKKALLPLLIEKVDTSIAKSIMSQLT
                     DISFDSVLPAFRPSIGDPSYQKTNINLNKYLSLVGNKTDISEFLILTIGLFTSLKIGD
                     KGFLQELSADYLFVRYDDCLHKIIEKLKEKEVIEQLGEKEVQKILEASGNLKRFKPTS
                     NNPRRFFETRLAQYINGLSHSEKTIEIDSIGEDKEPEDEELRDNTLKACNKILQFFLG
                     DCGRVDTPREMEQKICIFANGYKDTSKFTKKEVEFITGVLHHAGAHKGRDRVERDKLT
                     GIKERKFETDSEKVGSDVWNCGGGVMKGCSPVFYDAQRDPMRNMLVDSSTVAPDNKEG
                     KQWFWNNNRQYSSYVGSISGHTCNIVGMLAKYMTEYKEDLDLQNDINLFLIQVIGVYA
                     KRGFHAMLEVIDVLHDPYVQDIFKGYGVQVNLYSYFKENPELAGFLQHAMNDATTYTQ
                     ALVNKKHVKEALKSNSFFSKKDEESGDKKNDHTPKDGTVYGC"
     gene            complement(13800..14849)
                     /gene="ceg15"
                     /locus_tag="I9760_001119"
     CDS             complement(13800..14849)
                     /gene="ceg15"
                     /locus_tag="I9760_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg15"
                     /protein_id="HAT5042895.1"
                     /translation="MADHSPFIKFMFLNIKLSILQYNIILKAIIKLKVAILMTNCKTS
                     NTLLLKPILGYSCFNFSISVFILILFYIPLTSGTCWAKKSVNHNEVVMRGKKEFLKHE
                     YSPGHWSIDYTRAGTSIAVITVRNKYHYSVILNPTDCRGYRIIIRYLNEGDSTLSSAF
                     NRPYTVSEQRGLNDVASLMTQVYEKLGLIVQFSQLGNNSQSFDKGTGVTLIGSEEEPS
                     MLHLHMWGRGDPDMEYIAGVPLRGPEPGLMFDLIAKNKTHPINQHAIKWNEEELKACL
                     AMFKLKLAEYVNSPEFTEEFGDTLKVTIHDKKDLGEAKESSFGFFHKMKSASDGITTL
                     EKTLALEDDKINPFA"
     gene            14941..15153
                     /locus_tag="I9760_001120"
     CDS             14941..15153
                     /locus_tag="I9760_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042896.1"
                     /translation="MDIHSTYKMLAKMQGNKELSSHKIAETEQAEADTVPQLKLQAEP
                     EAKPDMQNILSNMLGISSNKKHSPMD"
     gene            15324..15584
                     /locus_tag="I9760_001121"
     CDS             15324..15584
                     /locus_tag="I9760_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAT5042897.1"
                     /translation="MAGGFSATSHWRDSARSARFFVVDARAAFPIFLFLMHIRVWTGV
                     LVLVSAIFFGIIEHYGFTVPVFLRWLRSFLAGSVRSARPWWR"
     gene            15585..15929
                     /locus_tag="I9760_001122"
     CDS             15585..15929
                     /locus_tag="I9760_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmS"
                     /protein_id="HAT5042898.1"
                     /translation="MERDISKCMAKIAASMNAKFYLNDRFVSFDEVFSETGLLPAIAK
                     RADQLCSLCLGYGLGATYDESEGALLGIRVVFDEVTPNVLRLLCMTDVMNELIQGGPS
                     RDYTPLDELMYD"
     gene            16029..16391
                     /locus_tag="I9760_001123"
     CDS             16029..16391
                     /locus_tag="I9760_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAT5042899.1"
                     /translation="MGNNTDDSARNPFGFYTPPRVKEIGEPDVTDATLGSVYSEIISP
                     VKDCILTVAKAVSFNPGGKDNTDAVEVLTELNTKVERAALNQPILTTKTERMFGAAES
                     EKSSEPPSHDERGFKLSS"
     gene            16482..17057
                     /gene="icmQ"
                     /locus_tag="I9760_001124"
     CDS             16482..17057
                     /gene="icmQ"
                     /locus_tag="I9760_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm secretion system protein IcmQ"
                     /protein_id="HAT5042900.1"
                     /translation="MKDQLSDEQKETILKALNDAIEKGPWDKSNFLRVIGKKLIAIRD
                     RFLKRIGAASQAKLKAESHLANRIALRSGQQEIYVSLYSSDGSNLQSWEKIVGSLPRQ
                     MISRPIYADEEDIKAILKTKENKQNEAYVAIYISQSDILHLSADKAPVDKLGKPLLTL
                     KDKSISLENISRFVHVSGVYRYSNGRLIKNA"
     gene            17244..18374
                     /gene="icmP"
                     /locus_tag="I9760_001125"
     CDS             17244..18374
                     /gene="icmP"
                     /locus_tag="I9760_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system coupling complex
                     protein DotM/IcmP"
                     /protein_id="HAT5042901.1"
                     /translation="MAQQQQQSGSDNSMAPVWIVILLFITAYFVWALAHQYIVSFVFT
                     INIWQARLVNLFLNNQLLANQIYLMQTLDPNTVNWDQMVTVMRAVGDYMRYPVICILV
                     VLAFVLYNSNVTLKYRKTYDMKSLRAQEQFNWPAIMPIVKEDLVSQDVNKGPWAMALT
                     PMEFARKYNLLRKDDALLDNPVPGEEMTAGIRRGDAKRVFTMQLGPYWDGFERCSPQA
                     YALSAVFMARMNRDRDAANNILKVLDKTFVDGKPDFSVARPVMKKYQNSELVQEVVAK
                     HAYVLTVIASLLEAAREDGVVPSSEFLWLKPVDRRLWYMLNCVGRQTPYSEVAGPFAH
                     WKAEKEMGRRSLVPMIDEAIRALEIAVKEVRLTPRQMEELEP"
     gene            18371..20722
                     /locus_tag="I9760_001126"
     CDS             18371..20722
                     /locus_tag="I9760_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAT5042902.1"
                     /translation="MMRGIDSRHELDPTLLLRDTRTFTQRLADFFADPTNISIVLISL
                     AAVSYYFSEAATFLLIMGGIFFLYSYTRKQKLPFRLPQISRAKDYNDLKPGINKPNIA
                     RGITFFGNDRKTGEELWFANDDMRTHALIFGSTGSGKTETLVSLSYNALVQGSGFIYV
                     DGKGDNSLYAKVFSMVRSMGREDDLLLINFMTGARDIVGPQEKRLSNTLNPFCQGSSS
                     MLTQLVVSLMGSSGQSSDGDMWKGRAIAFVEALMRLLVYMRDEGAILLDANTIRNYFD
                     LQRLESIVIDKVFPRDDQESVNIETIPKLVTDPLRNYLNTLPGYNKEKKGKQVSQVLE
                     QHGFITMQLVRSFSSLADTYGHIIRTNLAEVDFKDVVLNRRILVVLLPALEKSPDELS
                     NLGKIIVSSLKAMMAAGLGEEVEGDYRDVILRKPTNAPTPYMCILDEYGYYAVQGFAV
                     VPAQARSLGFSAIFAGQDLPAFQKASKEEAASIGANTNIKICMKLEDPTETWDFFTKT
                     AGEAYVTKVDSFQTKETSIANSYMDTKSSSFEKRARVDLLDLKEQTEGEAHIFFKSKI
                     VRARMFYANPKPVKQLKINQFLKVEPPPDDYLMKLQKQLASFQSILESGDLSINKAVE
                     NEEITLISKALKESTIVEPIERGVAALIAFHGQNEPEPVEDIVEEEVEGALTIFSKLR
                     IDPNAPPILVADKEVFSEPLLPINETRNQMITIERLAGAKDKYAGTVANELIKDFQIA
                     TSYPPEERDVIDVQELTGIIRDLSAKISAEREKANKKAAEELT"
     gene            21126..21695
                     /locus_tag="I9760_001127"
     CDS             21126..21695
                     /locus_tag="I9760_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAT5042903.1"
                     /translation="MRSLRTNYIYVLFKTTGLLFLLLLSACNRSGYIPENEVPKLPCR
                     VDGACDATIIKMMTDLNKKGIKVASVGQNYLISIPASALFADQSPRLNWASYSLLNEI
                     AAFLKQFRKIAITVTSYSSKYVSVKRERALTLARSRVVSEYLWSQGVDSRIIFTQGLG
                     SDKPITSYTLGGDRSPNARVEITFRRAVA"
     gene            21707..21991
                     /locus_tag="I9760_001128"
     CDS             21707..21991
                     /locus_tag="I9760_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAT5042904.1"
                     /translation="MSRETWALIKRNKNFNVHIYRRGLTLLILSLGLSCGLSGLIYYV
                     HMHEPERDFYATSGITPPIQLKPMSTPNMSSEALLPPDPPDEDSQRIIPQ"
     gene            22007..>22265
                     /locus_tag="I9760_001129"
     CDS             22007..>22265
                     /locus_tag="I9760_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein DotI"
                     /protein_id="HAT5042905.1"
                     /translation="MAEDALTVVALRNKFYKDSQRKVILALLIAIVVNVVLASLVVYM
                     ITHPPAPKYFATSINGRITPLFPLDEPNQSDSAVLQWANQAA"
BASE COUNT         6905 a         3748 c         4444 g         7168 t
ORIGIN      
        1 tgagagacaa aaaaaatcat taagaaaaag atccttttca acataatctc ctgatagtag
       61 ttagttttaa aaaagaaggt cgccatagta caggaaatga gtttttcaga ccatagtctt
      121 tagggcgggt tattgtaaaa tgtgtttaac atcaattact ttttgccttg accctggttt
      181 tttgcaagtc cttactcaaa gctttctcag attaagccct gtctttgata aggtgattgt
      241 cttcaatgga ttgatactca agaagatctc cgggttgaca atgcaagtaa gcacaaatag
      301 cttcaagggt tgcaaaacgt attgctttgg ctttaccatt ttttaaaatg gataagttag
      361 cttcagtaat gcctatagct tcagctaaat ctctaagtcg acactttctt tttgccatca
      421 tcacgtccaa attaacaata attgacatcg attgaaactc cttaaacaag cgggatataa
      481 actgattgag actgcgtaag aaaatctctt gatgtcctta cgctgaagtt cacattaaat
      541 ggttaacaag gcatccgttt ttaatttatg tgcttcctta ataatccaag aggcaatcaa
      601 aatgataaaa gcggtaatta acgtggacag gttcgctgtt cccaaggtga tgcttgcgat
      661 acgttgacca gatggattgg taaaagtcag agctgcggtt atcaaaggtt gataaagcaa
      721 ttgtataagt tcacctgaga tcaggtaaat actgatattc ttaatcagtc gaatgttttc
      781 ttcttcaaat aaatagccat gttcatacaa gcgaaacagc tttgccaatt gatgacaaat
      841 tagaacatta atggataagg gtaatgcttc aatcaataaa ataatgaaac gatgcaacaa
      901 ggaaaaatga gaagggtctt tcactggggt tgataacagg gagtgccatc ctccccaatt
      961 cagcatgctc tcgaaatgaa atagaatgaa ataagtcgtt acaaggggaa ggccatatgc
     1021 taatgttttg aataatagaa aaagcaaatg actcacagat tttatttttt tcattatata
     1081 attgtccttc taagatgaga tcatgcgtat tttattaaat atttattgtt ttgcaataaa
     1141 tatttaatgt gaaacataat tttattgcga ctgccgatgg ttaaatcagt aagacggcag
     1201 gttaaaattg ctcgttaaca atactttgaa ttgctttgat ccactcagga tgatcgttca
     1261 tgcaaggagt taatataaat tgctcaccgc caagtttttc ccactgttct tttgcacgaa
     1321 ttcctatttc ctcaagagtt tccaggcaat ctgcgacaaa agatgggcag gagaccacaa
     1381 tgtttttaat tcctttgctt attagttcgg caaaaatttc atcggtataa ggttttatcc
     1441 atggagtttt cccaagtctg gattggaagg cggttgtgta ttggtgagtg cctaattgca
     1501 attcttttgc cagtaataga ctggtctgat agcattgagc cctataacat gcttgatttt
     1561 tatcgtatat gggtgtgcat gtttgggggc aaagcgtatc gcacccgctt ttgtgaatat
     1621 gccgttcggg aattccgtgg tagctaaaaa gaaggtggaa attgtcttta atataaggct
     1681 tcataatttt agcttgcgcc tggatgtatt ctggtctttg gtagaaatct cgaatgattt
     1741 taattgatgg aataatttcc tggtttgcca aatgggataa ggttttttca atggctgaac
     1801 ctgtagcagc agaagaatat tgaggaaata agggtaaaat ggtaagggaa tggcaatctt
     1861 ttaattctgc aagtgctgtt gtaattgatg gagttccata gcgcattccc aaagcaattt
     1921 tgtactcatc cttgagtgcg gactgtaatt ttgtcactag attttggctg tgatacaaaa
     1981 gcggagagcc tttttcagtc catattgatt ggtaagcctg ggctgatttt ggcgatctaa
     2041 agggtaagat caaacaatat accaggatat agcgcgggat agttggtaag tctattactc
     2101 ttttgtcagt aagaaattca cgcaaatata actttaccgc cctgatatct gcattattgg
     2161 gagtgccaag atttaacagt aataaaccac gtctcatcag ctaaagtcca gttataaaat
     2221 gaaggacata atacctcaag ttgatggacg cagcagcata aatattagtt atactgccgc
     2281 gcttaatagt ttatcacatc aatctggtta agctatttct tcttgttcgg cgttttcact
     2341 aagagcaacg acattagagg catgtaaacc tctttcacca ttaacgacat cataggtgac
     2401 tgcctgccct ggaaccagcg ttttgtatcc cgtgccgtga attgaggaga agtgaacaaa
     2461 aatatcttca ccaccacctt ccgggataat aaaaccccaa cccttggcgt tattaaacca
     2521 cttgacttcg cctctagcca tgaatcctcc ttatgctaaa gcttatcaat cgtcgactga
     2581 cataagcggt cagctgagca attgcattct tccttaaatg acaattgtta atagcctatt
     2641 ttacaatagg tcatttcaac ataacgcaaa aagggatcat tactcaatag gtttattaaa
     2701 atttatctgt aattcttata attttgagat catgtttttg ttacaatgat agagagctta
     2761 ttgatagaga gagtattttg ttttgcattg atgatcggat taagtcgtct tcttttatca
     2821 ttggagagtg gcctttatct acagttttat taaaaaatga cgcctcatat ccctggttta
     2881 ttttggttcc aagaagaatg gatattcaag aaattcatca gcttgaaaag agtgatcgat
     2941 ttttattaat tgaggaaatc aatcaactgt ctcttctggc tagtgattat tttaaaccag
     3001 acaagcttaa tataggcgct cttggcaata ttgttccgca attacatatc catattgtta
     3061 tccgcagaaa aagtgatcct ttgtggcctc aaggtatttg gcaagcttcc atgccttcta
     3121 ttccttaccc tgaaagtgaa ttaaagagtc tgctgcctac cttgggtgat ttagtcgggg
     3181 cttacgataa accccaatct ctttgttaaa aaatgttttt aacttggtca tttagtttat
     3241 ctaaactacc tcattaaaaa cattttttgc ctcgaccttg aggttcatcg taagtcctgt
     3301 tagtaaacaa ggcatactaa ggaacattat tattttgtat ttttggaaat attgatgtct
     3361 ctcataacac caatacgtgt gaggctctct ttagggattc tcacacgtat tggcagcaac
     3421 aatcatgtta attgatgcaa agtgaacgca tttccctctg aatccacagc aatcgccatt
     3481 ctgcaaacag gtgaagcgaa taggtcaatt ttgaactgaa ctcctttgga ctttagttgg
     3541 aataccagat cgtcaagatt atctacctca aaagcaacgc ttcctccgga atgagcgcta
     3601 gggattactc cttgagccaa tgtagtaatt gcaaaacaac ccccttgagg taaatcatat
     3661 tctacccatg ctccctcagc ggaaatttta cccacttcaa gtccaagtgt tttttcataa
     3721 aattgccttg ctctatccat attttgaact ggatacatgg taaatgctat ttttttgatc
     3781 atgagggctc cttgttgaaa tttaaatata ctttcaccat tagatgaatt ataaattaca
     3841 atgataaaca ttcactttga aataaacgat gatgtgcatg cttaaaaaat taatgttctt
     3901 attattgttg ggagcaggtt tgttaagctg tggtcaaagc acggatcagc aaatttccaa
     3961 tattataaca ccgaagcaat tcccctccag tcccatggat tatcgaccag ttcaggaatt
     4021 gccttatttg gcctggtgga agcaatttca ggatccagaa ttggatcgtt taattgatac
     4081 tggattgaag accaatatgg atattcatat tgcaatggct aatttacagc aagctcaagg
     4141 agagttacag caagtgaagt tgagttggat acctaatgtt caaatattgg gagggtattc
     4201 aactaatcct gctcttggtg tgccaggagg gttttatgga atatggccaa gttatatttt
     4261 gaatattatg caactttata ctcagcaaaa gcgcgctcaa tatcaggtcc aatatcatca
     4321 ggcagcaatc gatggtttgc gtttgacctt gattggtcag gtggcagcgg cttatttcac
     4381 tttgatcgca cagttagagc aacttaaatt attgtataaa ctggataggg atttaaagtc
     4441 gttaattgct ttgagcagga aagaaatcaa cataggatta aaaaatgata ttgatttggc
     4501 tcagttgcaa tccaacgagc gcttgattgc agcacaaata aaacccatta aacacaacat
     4561 tgtcgttagt cagaatgcgt tgcgttattt aataaatgag aatcctggtt taatcaaaag
     4621 caaaaataat tttgctcaat tggattttac ccgctttaaa cccggaagtt tacccgctac
     4681 tgtattaggt aatcgccctg atatgaagat ggcggagtat tccctaaaag cggctcgtgc
     4741 aggtattttt gttgcttata gtgatttttt tcctgttatg cagctggatg attttattgg
     4801 agaggcacat ttgcctgata gcacgtttga acaagcgacc gatgcgtacg taaactggac
     4861 aattaatccc aatactctgg gtaaaatttc agttagcaag ggagtttata atgcaaaatt
     4921 ggccgaatac gttaagacag tccgattaat tttaaaagaa gttgataatg ctttttcagc
     4981 taataaacgc atgagagagc aatttttatc ttatcggcac gcacaagaag attatcagca
     5041 taaatacaaa ttacaaaatg gtttattaaa atcagggtta atgtcctata aagagttatt
     5101 ggagagtaag gtttatctgg ataccctggc actctcaaca aatcaggcta agctggaatt
     5161 ggccatgtca ctggttatgt tgtatcaaga tttggctggt gggtatgcct gtagtgacta
     5221 aatcagtttg ctttgtaaga cattgatttt aaaagtcgcc aggcgggcta aaattgcaat
     5281 tctcgtctct tgagctggtc gatgagataa gttaatttcg cattattgtt atataccaag
     5341 ggaaactggt tggctatttt atgaaaaaat tgatggaata tggtggaaaa ataaaagagt
     5401 acttgcttcg tttaaaacca aaacgctggt ttactcttac taatgtcatt atttttatcg
     5461 gttttcttac tgcaattatc tatgtttttt cctatttgat cccttttact gataatgcgt
     5521 ttgtagtaaa caatgtacgt ccggttgcgg cattaacaag cggatatata actgagcttt
     5581 atgtaaaaaa tggtgatgca gtgcgtaagg gacaaaaact atttaccgta ttcaaaaagc
     5641 cgtatgaata cacagttgaa cagctcagtg ctgatttagc tggagcacag gcaaagttgg
     5701 cagtattaaa agctacgtac gaacgggatt tgaagcttag cgggaatgag caaaaaatat
     5761 ataaaaagtt ggcgcaagat gatcaaaaat accttaaagg gtatgctata aaatctgttt
     5821 ctctgattac cctgcaaaac tcccagcaag aaacaaaagc agctaaagat aaatggcagg
     5881 cttcattaaa acaacttgaa atagatcagc atcaaataac agttcaggag aatgaaataa
     5941 aatccattca ggcccggtta aaaaatgcca aagtcaattt ggatttaacg gatgtttatg
     6001 ctcagggtaa tggagttatt caaaatttgt tttttaccat aggaactcca gtcaatatca
     6061 atcagccatt attctcattg gttgatcagg ataatattta tattcaggcc aattttaacg
     6121 agacggattt gcgtgatgtg cgcaagggct caaaggtatt gatatttcca agaatgtatt
     6181 taggaagaaa aatatttcat ggtgttattg attcggatta ttggtccgcg aataggcaat
     6241 tggtagatga tcgcactcag ctgcaaaatg ttatcaatga aaatcaatgg attttattgc
     6301 cccagcgcct tcctgttatt atcaaggtta ctgaccctga ccctaaatat ccgcttcgtg
     6361 taggagctag tgcttatgtc tatgtcgaag tttattgaat ggattgatga ggttgatcct
     6421 tatgctgttc aacgaatagc gctctataaa tcacttttta ttgccaccgt tatggcctat
     6481 gtttattggg tatttcgtcc cacgaatttt acggcatttt tttcaccatt tttgctcgta
     6541 agattttatg aatcacccag tcttaccagc tttaaagaga aagaacactt tttgattttt
     6601 attggagtgg tagtggtatt gttgagtacc tcattttatc ttgtttaccc atttcgggtt
     6661 gtatttttct ttttctcaat tattgcgtta gcaagcgttt atttttatgt gctaaaaaat
     6721 tatcttccat tgaggaatgt gactatgctc attattgctt ctggagcgac aattttaagt
     6781 acagaacctc ctgcaaattg gcaaattgtc tacgacatcg ttgggtcaag cgctttatct
     6841 atgatcgcca tattcatttg tttaagattc ttccccaatc aatatctcag tgtatggaaa
     6901 agggctttgg caaaatttat tcaatccctt gagctggata ttgaaggttc ttttactcac
     6961 cgtggtccca aattcatgca ggaagaaatg actcatttgg gtatgcttag gcaatatcgt
     7021 agacttatcc ccaaaaaata catgatttat actcagcgaa tttcaatcaa catacgcaac
     7081 attcaatttt ctttggataa tctttattat gaagccaaaa atgaggcttt ctggcatgga
     7141 gtgaaagaga atctttatac tcttagatgt gcgattaaaa ccaacacatc ttgtggcact
     7201 cctaaaatgt ccatcatgcc tgagtcaaaa ttacaacaat atgttatgag atgtttgcaa
     7261 caagcattta gccaatggaa tcaattatgc atgatacttc agcattgaag aaaaaacttg
     7321 attatgctcg atttattcat ctagtcatca tgttcatggt tgccatgctc atttttttgt
     7381 tttcgacttt tcccgaaaag tggtgggtat tgatcaccgt cttgtcagta agtgcgggta
     7441 ttgaaccagg attgattatc agaagagcta aacacaggat aggagggaca ctgttggctc
     7501 tcatcatttt gattccctta ctctatttat tgcaattgaa ctatcgattg atatcaatct
     7561 tatttgttct ggcgatagtt ggtttgagtg ttgctacctt aaacacaaga cgttacgaca
     7621 ttagtgtatt ttttattact ttagttgtct tttttttgat ggcacaaact gaagaggcga
     7681 catcgcctcg tggtcctttt gagatggttc ttaatcgagg catatgtaca ctgtttggtg
     7741 tttttattgt gttggcaggc gattattttt tatttcaggc ataccgttat tctcataggc
     7801 tttatttttt tcatcaggtg atagtttatg attttttaaa tgatattgtg cgaaaaatag
     7861 acgaatccaa tacagaaaaa attaatacat tgctctttgt agagaaatta cgtggccaat
     7921 tcaccgagca ttatctcccg atatccatca gctccgaaaa tttgaaactg gattttaaaa
     7981 caagtgaaca cactaaaaaa agaattgata tttttcaaga aacgatttgg gaaatacgac
     8041 gcttggtttt tgcattatgt atttcagagt ttgttttaca ttcctcaacg gcatctgaac
     8101 agcatttgca acgatttaag ttactgatca ataaagctcg aacccatttt attcagtttg
     8161 aaggtcgtta ttaattagaa atagggagta caaatatgtc tcatcctcat tatagtgttt
     8221 ataacgagaa gtgcccatca caaaatgtac ttgaaggtat aagtgataaa tggtctatat
     8281 tggtcattag ccttttatcc caaaaaatat accgttttgg agagttaaaa cgtgaaatcg
     8341 gtggaatatc acctaaaatg ttgacgcaaa cattactgaa attggagcga tatggctttg
     8401 tccaaagaca atcttatcct gttttaccta tgaaggttga gtattcatta accagattgg
     8461 gtaaggaatt aagtgccata ttggttttac ttactcgttg gactgaaacc aatatggata
     8521 aaataatcaa tgcggaaaaa acattttgcg aacagaggct tggttgtcat gcttgattta
     8581 ttgaaaccat ttgattaaag tttccgaacc taaatggtca tggatatagg ttaaatagcg
     8641 ggtcaactcc agagtataat cctcttcaga aacaggggta ttttccccaa ttaaagggtc
     8701 atgatgaaaa aaaattccgt tgagacgggt atcacaaaac tcatgataat ctctggtgaa
     8761 taaaagaaaa gtatgccaca tcttgtcaat atcagccatt tcagcatgaa tgacacaaga
     8821 gaaatccaga tttttatcat caggataaag taatttatct gttttatgtt tatggcagag
     8881 ccaaataaat ttcattaatt catgtaatgc ctcttcagct tgcatggctg cagttgggta
     8941 atccatatta tatctggtaa taattttatg atttctgtaa tcaagtaagt cttttaagtc
     9001 aggatgcatg attatggggc tttgattagt ataagtgtaa cggcacgttt tttggtaacg
     9061 tgccatcatt gcagttatat catccaatac aagttcctgg gccacaccat cctctattcc
     9121 tttttagcag ttttttctgg acaattttct taatgagttt ttctttcata attcactcct
     9181 ttattttata aaatgaacgg gttcgagact aaaatattta ataaaatcag tcaattagtt
     9241 accttttggt aactagaggg gatacagtaa atgaataaaa gaaattggac ggcttattat
     9301 aattctacga aacaaaacac tctccccaga aagtctttgc ttaaagcaat tgcaaatttt
     9361 gataaggaaa agatcaatct atctaaatcg gcaatcgatt tgggatgtgg tgctggtatc
     9421 gatgttatgg aacttttgcg atgcggttgg tctgtgattg ccattgattc acaattatct
     9481 gctatagaca gtttgctttc ttcagctcaa gaattaacat gcgcaaacaa attaaaaaca
     9541 ataatttctt catttgaggc tttggattca ctccctaaag taaacttgat taacgccagc
     9601 tttagccttc catttctcaa gccagtttac ttttataaat tctggagcgt tattttaaaa
     9661 gggcttaagc caggtggtcg attttctggt tcgttttttg gttttaatga cttctggaac
     9721 accagaacgg atatgacttt tttaaatcgg gaaatgattt gccatttgtt ttcccaattc
     9781 aaaattgaat attttcaaga agaagaaaag gatgaaatgg atgctttggg ttattataag
     9841 cattggcata aattttttat tgtagccaaa aaaatcgcat aaatgggtaa tcataaaatt
     9901 gtatgtattg gcttagaagg cacaatcaag ttcttatgag ttgagagtta tatatgagag
     9961 ttatataaag gagagaggcg ggggtgccaa gaaacgccaa atagtaaatt tggtgcttct
    10021 tgacgactta atagatagga taaaaaattt aaagtgcgtt tttaggggta tctatgccag
    10081 cttcttcaaa acgactctct ggaacttttc ttgaaggagg tatttctcca ccggtcataa
    10141 agaaaagact aatggaatct tttaaatttt gttgatcgta tgtttctttt gagctgaact
    10201 gccccctaat tttattgagc agattggtaa gtacttcagt agagccatgg atggttggag
    10261 catctacttc tctatcaaca attggataaa ctgattgcag caattgcatg taatcgtact
    10321 ttactatacc accgaagcga ttgattaatt gtgatttcct tgcttgttct ccaaagcgat
    10381 taccttcagc atcacattta gaagaaacag cttccaaacc acccattaat ttaataattt
    10441 cacaggtgtt tttaatttta tcttcagtca ggtatttgat attggtgcta ttcaccagca
    10501 ggaaatgtgg tgcttgttct gaaatggggg cattgtgttg gactaagtac ttgaataatt
    10561 gaagatcctg atttttacaa gccaattcaa ttggtgtctc tttgtgatag ttgataggat
    10621 aatcaaaatc aacatggtgc ttaaccaaaa tatcaagcat tttgtagtca atgtgttttt
    10681 tatttaatac tctagccagg ggtgaatcat aagtgggggg ggcgtactga tttacaagct
    10741 caggatcttt atccagtata gtattgacag tttcataatg accttcagat actgccttca
    10801 tcaattcacg caattcagct gatgctattt tcatttcact tctacccatt ttaatcacct
    10861 attttaaact atcgccatag tattctaaaa atcaaactgg ctgaatctta gcatgaagac
    10921 tgtataaatg acaatatttt tgttcattta ttttacttat taataattat ttagatcaat
    10981 tcaggtggta atttaaataa acaacacttt tttttaattt tgaacaataa gtttaatatt
    11041 ttcttaatct ttgtgtttta tcataccccc tgtgaaatat aagttgtcaa acatgacaat
    11101 gctaagaggt agagggttat atgcaaaact tagatgagat tctaaagaaa ctgagaaaag
    11161 atctgaaaaa gacttcgaag gaaacgactg agaagctcag tgttttatca aagaaagttg
    11221 ctgaagatgt ggttaaggta tccaatcaaa cggttcaaaa aattaacgaa gtaaccaaaa
    11281 aattcaccgt agatactaca gtcagcacta ttgacaccca aaaaatttta gatgatttta
    11341 tgaaaaatat acgtttttct cgatttaagc agatttggca agcaattgag aaaacaccga
    11401 aatccaagca tatattgtta aaatctctct tcttaaaagg gctattgact cataataaac
    11461 ctctacttga agaaattata cgacaaattg aattaatacc ttactcaagt gatctggaga
    11521 tgctcggtgc ttttagtcag gacaaagcac atccgctctc acctgagctt ctggagtttg
    11581 tgcaagagat gcttgccaat gaaagtgaga aagtattact cactattctg atgaatgctt
    11641 ttcagtctat tcctgaattg tctcttgata gcaagacagg gactttaagc atgaaaacta
    11701 aaaaagcctt actgcctctt ttaattgaaa aagtggatac ttcgattgca aaatcaatca
    11761 tgtcacaatt aactgatatc tcatttgatt ctgtattacc cgcatttcgt ccatcgattg
    11821 gtgatcccag ctatcagaaa acgaacataa acttgaacaa atacttgtct ctagttggta
    11881 acaaaacaga tatttcagaa tttttaatat taacgattgg tctttttact tccttaaaaa
    11941 ttggagataa ggggtttttg caagaattat cggctgatta tttatttgtt cgatatgatg
    12001 actgcttgca taaaataata gaaaaactaa aggaaaaaga agtaatagaa caattaggag
    12061 aaaaagaagt tcaaaaaatt ctggaagctt ctggaaacct taagcggttt aaaccgacaa
    12121 gtaataaccc aagacgattt tttgaaactc gccttgctca gtacattaac ggcctttccc
    12181 actcagaaaa gaccattgaa attgattcga taggagaaga taaagagcct gaagatgagg
    12241 aattgagaga taacacattg aaggcatgta ataaaattct gcaattcttt ttgggtgatt
    12301 gtggaagagt tgatactcca cgagagatgg aacaaaaaat ttgtatcttt gccaatggtt
    12361 ataaggatac ctctaaattt accaagaaag aagtcgaatt tattaccggt gtattacatc
    12421 atgctggagc gcataaaggg cgcgataggg ttgagcggga taaattgacg ggcattaaag
    12481 aaagaaaatt tgagactgac tcagaaaaag taggttcaga tgtatggaat tgtggcggtg
    12541 gagtcatgaa aggctgttcg cctgtctttt atgatgccca acgcgacccc atgcgtaata
    12601 tgctagtgga ttcatcgact gttgcgcctg ataacaaaga gggaaaacaa tggttttgga
    12661 ataataaccg ccagtattcg tcttacgtag gatcgatttc cgggcatact tgtaatattg
    12721 taggtatgtt agccaagtat atgacagaat ataaagaaga tctcgatctg caaaacgaca
    12781 tcaatctttt tcttattcag gtaatcggtg tttatgccaa gcgtggtttt catgccatgc
    12841 ttgaggtcat tgatgttctt catgatcctt atgtacaaga catattcaaa ggatatggtg
    12901 ttcaagttaa cctttatagc tattttaaag aaaaccctga gcttgctgga ttcctgcagc
    12961 atgcgatgaa tgatgcaacc acttatactc aggctcttgt taataagaag catgttaagg
    13021 aagcactcaa atcaaattca ttcttcagta agaaagacga agagagtgga gataagaaaa
    13081 acgaccatac gccaaaagat ggaactgttt atgggtgcta aaggataatt tgggttccca
    13141 ttccctaata gcctcggctt gtccgaggca tcaaattcag ggaaattttg cattaagaat
    13201 atatcttttc gaataaatca cccctggaca agattgattt ttgctctatt gtctagctcg
    13261 tttttaaatt tttttaaaga caagcaactt ctcttctcta taggtttcgt ggctttcata
    13321 atagacctat agcttgtact tcgttgtatg tgttgatgca ctcttttgcc gagacgaata
    13381 gactgctttg cataacctgt tatttttatt ttactgccgc gttgcgaact tttctgtggt
    13441 gcttatttaa agacgtttgc cccttgtcct gaaacaaaat aaacaggtta tgccagaggc
    13501 ttaatgctaa taaatgccac tctggtaggg tgtatacaag tgctttaaac atattcatca
    13561 gttttttgat gaccatcaat tgagctgttg ataaattgaa ttcgaacaga taaaaaatct
    13621 gcagatatgt gtttcatagt attatccact atcaagttgg tagtagcaac aggtagacaa
    13681 gtgggtacgc atggttaata gtcgctggct gaaaacattt ttatccggaa attttgacac
    13741 ttttcctggc tgaaattcga gtacaataaa gagacgatac catttatgtg ctctgtcgat
    13801 tatgcgaagg gatttatttt atcatcttcc agggctaagg ttttttccaa agttgttata
    13861 ccatctgatg cagatttcat tttatggaaa aacccaaaac tactttcctt cgcttcacca
    13921 agatcttttt tgtcgtgaat agtcactttt agggtatcac caaattcttc agtaaactct
    13981 ggtgaattaa cgtattcagc cagtttaagt ttgaacatgg caagacaggc tttgagttcc
    14041 tcttcattcc attttatggc atgttggtta atagggtgag ttttattctt tgcaattaaa
    14101 tcaaacatca aaccaggttc gggccctcta agaggaacac cggcgatata ttccatgtca
    14161 ggatcacccc tgccccacat atgcaaatga agcatactgg gttcttcttc acttccaatg
    14221 agagttactc ctgtcccttt gtcgaaagac tgggagttat tgccaagttg agaaaactgc
    14281 acaataagcc ctaatttttc atagacttgg gtcatcaaac ttgcaacgtc attcaatccg
    14341 cgttgttcag atacagtata agggcggtta aaagcagatg aaagggttga atcaccctca
    14401 ttcagatatc gaataatgat acgataacct ctacaatcag tagggttaag aatcacagag
    14461 taatgatatt tatttcggac agtgataact gcgattgagg tgcctgcccg agtataatca
    14521 attgaccaat ggccagggga gtattcatgt tttaaaaatt ctttttttcc tctcattaca
    14581 acctcattgt gatttacact tttttttgcc caacatgtgc ctgatgtcag cgggatataa
    14641 aacaaaatta aaataaagac acttatagaa aaattaaaac aactatatcc cagaatgggt
    14701 tttaatagca aagtgttact ggttttacag tttgtcatta atatggcaac ttttaatttg
    14761 ataatagctt tcaagatgat attgtattgt aaaatactta acttaatatt aagaaacata
    14821 aattttataa agggggaatg atcggccagt gccgaatttt tttgattttt ttcaaactct
    14881 taataattcc ttaatttgta tgtgatatct taattaagag ttgttaaaag gggatttcag
    14941 atggatatcc acagtaccta caaaatgtta gctaaaatgc aagggaacaa agagctaagc
    15001 agtcataaaa ttgctgaaac tgagcaagct gaagcggata ctgttccaca gttaaaactt
    15061 caagctgaac cagaagcaaa acctgatatg caaaatattt tatctaatat gttagggata
    15121 tcatctaata aaaagcattc tccaatggat taagtccggg gtattaacac ttaggatatc
    15181 tatttttggt taaccccttc gtgtttgcaa tattcaattg cataaaaatt aatcttagtt
    15241 taagtttctt aaaagtatca acgtgcacaa gctgtttttt aagtatatac tttttttaaa
    15301 ggatgatttt ttgaggttta aaaatggcag gtggattttc agcaacgagc cactggcgtg
    15361 actcagcgag aagcgcgcgg ttttttgtag ttgatgcgag ggccgcattc ccaatatttt
    15421 tatttctaat gcatataaga gtatggacag gtgttttggt cttagtttct gcaatttttt
    15481 ttgggataat tgaacattat ggtttcactg tcccggtttt tttacgctgg ctcagaagtt
    15541 ttttagctgg ttcagttaga tctgctagac cttggtggag ataaatggag cgagatatta
    15601 gcaagtgtat ggcaaagatt gcagcaagca tgaatgcgaa attttacctg aacgatagat
    15661 ttgtaagttt tgatgaagtt ttttcagaaa cgggtttgct accggccatt gccaagagag
    15721 cggatcaatt gtgttccttg tgtttgggtt atggtttggg agcaacttat gacgagtctg
    15781 aaggggcgtt actaggtatt agagtggttt ttgatgaagt gacgccgaat gtattacgtt
    15841 tgttatgtat gacggatgtt atgaatgagc ttattcaagg gggcccaagc cgtgattata
    15901 ctcccctgga tgagttaatg tatgattagt tgtttgtaaa gaattagaaa gttttattgg
    15961 accaagatat attgaaagat atattttgat atatgtaaag taagagattt agctcaggag
    16021 tggtaataat gggtaataat actgatgaca gtgcacgaaa tccatttggt ttttatacgc
    16081 cacctcgtgt taaagagata ggtgaacctg atgtaactga tgctactcta ggcagtgtct
    16141 atagtgaaat tatttcaccg gtaaaggatt gcattttgac tgttgcaaag gctgttagct
    16201 ttaatcccgg agggaaagat aacacggatg cagttgaggt gttaacagag ctcaatacaa
    16261 aagtagagag agccgcctta aatcaaccga ttttaacaac aaagacagaa agaatgtttg
    16321 gtgctgctga atctgaaaaa tcgtctgagc caccaagcca tgatgaacgt ggtttcaaat
    16381 tatcatcttg aaagattaat ttcatttcag tgatttttat aaatgctgtt atactttctt
    16441 tatatttccc ctaattcttg gttcccataa gtattgaagt tatgaaagat caactctcgg
    16501 atgaacaaaa agaaaccatt ctaaaggcct taaacgatgc tattgaaaaa ggtccatggg
    16561 ataaatccaa tttcttaagg gtaattggta aaaagttaat tgcaattcga gatcgttttt
    16621 taaaacggat aggtgccgct agtcaggcca agttaaaagc agagtctcat cttgcaaatc
    16681 gaattgcctt aaggagtggt cagcaggaaa tatatgtttc attatattcc tcagatggaa
    16741 gcaatcttca gtcctgggag aaaatagtgg ggagtttgcc ccgacaaatg atttcacgac
    16801 ctatttacgc agatgaagaa gacataaaag cgatattaaa aacaaaagaa aataaacaaa
    16861 atgaagcgta tgtggcaatt tacatcagtc aatcagatat cttgcattta tccgctgata
    16921 aggctccggt agataaatta ggtaaaccgt tattaacgct taaagataaa tccatcagtc
    16981 tggaaaacat cagccgcttt gttcatgtga gtggcgtata tcgatactcc aatggccgtc
    17041 taattaaaaa tgcataggcc gtttcggttt tccttataat gatttcgagc gtgttaaaaa
    17101 tttatttcta ctctaactgt ctttacgctt tatttaatca tccttgtatt catattttga
    17161 tatagtacac gaaaatctcg caaaaatagc tctgagcagt atatacttga tatatagaat
    17221 ctattaacta attgtatata gagatggcac aacaacagca gcaatcaggt tcagataatt
    17281 ccatggcgcc agtatggatc gtcatactgt tatttattac tgcttatttt gtttgggcac
    17341 tggctcatca gtacattgtt agttttgtat ttactatcaa tatctggcaa gcaagattag
    17401 ttaatttgtt tttaaataat caactcttgg ccaatcaaat ttatctgatg caaacacttg
    17461 atcctaacac tgtcaattgg gatcaaatgg tgactgtaat gcgtgcggtc ggtgattata
    17521 tgcgttaccc ggttatttgt atcctggtag tgttagcctt tgttctttat aactcaaatg
    17581 tgactttaaa atatcgcaaa acctatgata tgaaaagttt gcgggctcag gaacaattta
    17641 attggccagc catcatgcct atagttaagg aggatttggt tagtcaagac gtcaataaag
    17701 ggccttgggc aatggcttta acacccatgg aatttgcgcg aaaatataat ttattgagaa
    17761 aggacgacgc gttattggat aatcctgtgc ctggcgaaga gatgacagcg gggattcggc
    17821 gtggcgatgc caaacgagta tttaccatgc aattgggtcc ttattgggat ggttttgaac
    17881 gttgctctcc tcaggcttac gcattgtctg ccgtatttat ggcaagaatg aatagagata
    17941 gagacgcagc aaataatatc cttaaagttt tagataaaac atttgtggat gggaaacctg
    18001 atttttctgt tgcaaggcca gtaatgaaaa aataccaaaa ctcagagttg gttcaggaag
    18061 tagtagccaa acatgcttat gtgctcacag tgattgcttc tttgcttgaa gcagcacgag
    18121 aagatggtgt ggttcccagc tcagagtttt tatggctcaa accagtcgac agacgtttat
    18181 ggtatatgtt gaattgtgta ggcaggcaaa ctccatactc tgaagttgca ggtcctttcg
    18241 cacattggaa agctgaaaag gaaatgggaa gacgctcttt ggtgccaatg atagatgaag
    18301 cgattagagc tctggaaatt gcagtaaaag aggtgagatt aacaccacgt caaatggagg
    18361 aattagagcc atgatgcggg gtattgattc tcgtcatgaa ttagatccaa cccttttact
    18421 tagggataca cgtactttta cgcagcggtt ggcagatttt tttgcagatc cgaccaatat
    18481 ttctattgtt cttatttcat tagcagctgt ttcatattat ttctctgaag cggcaacatt
    18541 tttgttgatt atggggggca tattttttct ttatagctat acccgaaagc aaaagttgcc
    18601 atttcgattg ccccaaattt cgcgcgcgaa ggattacaac gatttgaaac cgggtattaa
    18661 taaaccgaat atagctcgag gtattacctt ttttggtaat gacagaaaga ccggtgagga
    18721 actttggttt gctaacgatg atatgcgtac ccatgcgtta attttcggtt caacaggtag
    18781 tggtaaaaca gaaactttgg tttctctgtc atataatgcc ctggtacagg ggagcgggtt
    18841 tatctacgtt gatggtaaag gagataactc tctgtatgct aaagtctttt ctatggtaag
    18901 gagcatgggg cgtgaagatg atttgctctt aatcaacttc atgactggag cgcgcgatat
    18961 tgtcggtccg caggaaaaaa gactttccaa tacactaaac ccattctgcc aaggctcttc
    19021 cagtatgcta actcagttgg tagtcagttt gatgggatct tcaggccaat catctgatgg
    19081 tgatatgtgg aagggacggg ctatagcttt cgtagaagca ttaatgcgtt tattggtgta
    19141 tatgcgtgat gaaggggcaa ttcttcttga tgcgaatact attcgaaatt attttgattt
    19201 gcaaagactg gaatctattg taattgataa agtttttccc agagatgatc aagaaagcgt
    19261 caatattgaa accattccaa aactagtaac ggatccttta agaaactatt taaatacctt
    19321 gcctgggtat aataaagaga aaaaggggaa acaggtatcc caagttttgg agcaacatgg
    19381 ttttattacc atgcagctgg taagaagttt ctcatctctg gcagatactt atggtcatat
    19441 tattcgtacc aatttagcag aagtcgattt taaagatgtg gtactcaaca gacgtatatt
    19501 agtggtttta ttacccgctc tggaaaaatc accggatgaa ttatctaacc ttggcaagat
    19561 tattgtttca tcattaaaag ctatgatggc ggcaggttta ggtgaagaag tagagggtga
    19621 ttatcgtgat gttattctaa gaaaacccac gaatgcccca actccttata tgtgcattct
    19681 tgatgagtac ggttattacg cagtacaagg atttgcggta gtgcctgcgc aagctcgttc
    19741 tctaggattt tctgctattt ttgctggaca agacttacct gcattccaaa aagcatccaa
    19801 agaagaggca gcttccattg gtgcaaacac aaacattaaa atatgtatga aactggaaga
    19861 tcctaccgaa acctgggatt tcttcactaa aacagctggc gaagcttatg ttacaaaagt
    19921 ggattcgttt caaaccaaag aaaccagcat agcaaacagc tatatggata ctaaaagctc
    19981 ttcttttgaa aagagagctc gggtagatct ccttgattta aaagagcaaa ccgaaggtga
    20041 ggcgcatata tttttcaaat cgaaaattgt tcgtgctcgt atgttttatg ccaatccaaa
    20101 accggttaag caattgaaaa ttaaccaatt tttaaaagta gagccgccgc cagatgatta
    20161 tttaatgaag ctacagaaac aattggcttc tttccagagc attcttgaaa gtggtgactt
    20221 gagtatcaat aaggcagtcg agaacgaaga aatcactctc attagtaagg cattaaaaga
    20281 atctactatc gtagagccaa ttgaacgagg cgttgctgct ttaattgcat ttcatggtca
    20341 aaatgaaccc gagcctgtgg aagatattgt agaggaagaa gttgaggggg cattaactat
    20401 tttcagcaaa ctacgaattg accctaatgc tcctccaata ttagtcgctg acaaagaagt
    20461 tttctctgag ccattattac caattaatga aacacgaaat caaatgataa cgattgagcg
    20521 tttggcaggg gctaaagaca aatacgctgg tacagtagct aatgaattaa tcaaagattt
    20581 tcaaatagcg actagctatc ctcctgaaga aagggatgtg attgatgtcc aagaacttac
    20641 tggtatcata agagatttat cagctaaaat ttccgcagaa cgtgagaaag ccaataaaaa
    20701 ggctgcagag gaattaacat aaagactcag tcggcaattg gaggtagaag tgttcctgtg
    20761 ctattgggca tgaagagagc tccatatgta agttactctg agttttttat aatccgggca
    20821 gtgacgttgt ttctttaaac tttgatctat caggttgata ttctgtgtgg ttcctctttg
    20881 ggagaacttt ttgtattctg aattaacagg gtaattatca gtttactcat agattaattt
    20941 ggcatatgtt gatttgcctt aattagtcaa ccccatctga aataaataaa ttatttaaaa
    21001 aaatggcaat tttattaaat tcattgtaac gtaatgattt gtatgttttt attatcaaat
    21061 ggaatgtttg agatagttag acatagataa cacaattagt ctattttcaa agatggggat
    21121 aacaggtgag atcacttcgt actaactaca tctatgtatt gtttaaaaca actggattat
    21181 tattcttatt gttgctgagt gcttgtaatc gttctggtta tatccctgag aatgaagtgc
    21241 ccaaattacc atgccgtgtt gatggtgcat gcgatgcaac aataataaaa atgatgactg
    21301 atttaaataa aaaagggatt aaagttgcat cagtaggcca aaattattta attagtattc
    21361 ctgctagcgc tctgtttgct gatcaatctc ctcggttaaa ttgggcttcc tatagccttt
    21421 taaatgagat cgcggcgttt cttaaacaat ttcgtaaaat tgctataacg gtaaccagtt
    21481 acagtagtaa atatgtgtcg gtaaaaagag aacgagcatt gacattggcg agatcaagag
    21541 tagtaagtga atacctatgg tctcaggggg ttgatagcag aatcatcttt acacaaggct
    21601 taggtagtga taaacctata actagttata ctcttggtgg tgatagatca cctaatgcca
    21661 gggtggagat cacatttagg cgggctgtgg cataattgag gtaaaaatga gtcgagaaac
    21721 atgggcttta ataaaacgaa ataagaattt taatgtccat atttacagac gtggcttaac
    21781 actgctaatt ctttctcttg gattgagttg tggtttgtct gggttgattt attatgttca
    21841 tatgcatgaa cctgagcgag atttttatgc aaccagcggc ataactcctc ctatccagct
    21901 taagcctatg tcgactccta acatgtcatc tgaagcattg cttccgccgg atccaccaga
    21961 tgaagattca caaaggatta taccgcaata atttttagag gatattatgg ctgaagatgc
    22021 cttaactgta gttgcattaa gaaataagtt ttataaagac agtcaacgca aagtcatttt
    22081 ggcgttattg attgctattg tggtaaatgt ggtcttggca tcgcttgttg tctatatgat
    22141 tactcatcca cctgctccaa agtactttgc aacaagtatt aacggtcgta ttaccccttt
    22201 atttcctttg gatgaaccaa accaatcgga ctccgctgta ctacaatggg ctaatcaggc
    22261 agcaa
//
LOCUS       DACTJE010000038        22037 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.038, whole genome shotgun sequence.
ACCESSION   DACTJE010000038 DACTJE010000000
VERSION     DACTJE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22037
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.038"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(75..1187)
                     /locus_tag="I9760_001130"
     CDS             complement(75..1187)
                     /locus_tag="I9760_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAT5042906.1"
                     /translation="MNIFNLPDLGEGLPDAEIHEWFVKEGDTVKADQPLVSMETAKAV
                     VDVPCPQSGTIAKLYGKPGDVIKTGEPLVAFVSTTEKPADKGTVVGNLEESTDVSEDN
                     FIIGSQRSSHRVKTTPAVRLLAKKLGVDLSSLKGSGDNGVITREDVQNQANINSQPPA
                     GFEHLRGVRRAMLNSMVQSHAEIVPVSIFDEADIHIWKPNTDITVRLIRAIIHASKKE
                     PALNAWFDTKHAARRCFKEVHLGIAMDNEEGLFVPVIHDAEKYSDSELRKIIDEFKES
                     VRNRAVSADKLKGATITLSNFGKFSGRFASPIIVPPMVAILAVGRLYKAAVINDEDKI
                     EAHKMLPLSLSFDHRAITGGEATRFLGAVIESLQQA"
     gene            complement(1190..2164)
                     /locus_tag="I9760_001131"
     CDS             complement(1190..2164)
                     /locus_tag="I9760_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAT5042907.1"
                     /translation="MPDITLVEAVTQALAYELAHDENVVVFGEDVGKNGGVFRATVGL
                     QDRFGENRVFDTPLAESMIAGLAVGMSIQGLKPVAEFQFMGFIYPAMNQIISHAARMR
                     NRTRGRLHCPLVYRAPFGGGIRAPEHHSESTEALFAHIPGLQVVIPSSPKRAYGLLLA
                     AIRNPDPVIFLEPKRIYRLVKQPVPDDGQALPLGKCFTLQQGDDLTLISWGASMHETL
                     QAAKQLTDEGISCDVIDVATIKPLDIETILSSVEKTGRCVIVHEGAKTCGVGAEISAQ
                     IMEHCMADLLAPVQRVTGYDTVMPYFQLEKQYIPSIARIKNTVMSIME"
     gene            complement(2157..3230)
                     /gene="pdhA"
                     /locus_tag="I9760_001132"
     CDS             complement(2157..3230)
                     /gene="pdhA"
                     /locus_tag="I9760_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAT5042908.1"
                     /translation="MTTVAQFEITYTQYLNEQGKLVAELPPFADNQSILKELYKIMVL
                     TRTFDKKAIALQRTGKMGTYAPINGQEAISTAIGHALKPEDVLVPYYRDYAAQFQRGV
                     KMSEILAFWGGDERGSHYSNNAEDLPICVPIASQCLHAAGVAFAFKYRNQPRVAVVCI
                     GEGGTSEGDFYEAMNVAGAWKLPVVFVVNNNKWAISVPIEKQTGSQTIAQKAIAAGFS
                     GVQIDGNDIFAARQVIGDAIEKARQGGGPTLIEALTYRLCDHTTADDATRYQPSHEVE
                     EAKAKEPIVRFKHYLMQQNIWTSQDEEKLVIECSETVEKAVEEYLNTKPQPVSSIFDY
                     HYAELPEYLIEQRAIAMEEYSHA"
     gene            3410..4732
                     /gene="pabB"
                     /locus_tag="I9760_001133"
     CDS             3410..4732
                     /gene="pabB"
                     /locus_tag="I9760_001133"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HAT5042909.1"
                     /translation="MIDSTLITLDYHDFSSESYEKFSRLPGFVLLQSTDKSTGRYDIL
                     SAYPYERMVIENNVHDKDLVLTELKNKLKIEESEFDLPFQGGAIGYVSYDFGSLLHNH
                     EQYSQKDLAHIPVLNFGFYDWAIIVDHHLKKATLFARNSHSSTKSVVDKIKNILNDQP
                     MKSVQFSVQAEFLPLISKENYRSSFNAIQQALRAGRSYQVNFTQPFQTKFTGDLWDMY
                     QRVNKNNPVPYAAFIRTEEADILSFSPERFLLYDNGEMLTSPIKGTIHRSKDPVVDKK
                     LKEQLLKCPKNKAENVMIVDLLRNDLGKLAKAGSVKVMNLCHIQSFNAVHHLVSDIRA
                     LCKEDIHPIEAFFSCFPGGSITGAPKLETMKIISEQETYKRGVYCGCIAYFSQHGRFD
                     ANIAIRTVTAKDNVLYLAAGGGIVIDSDWEDEYRECFTKITAIINGIK"
     gene            4719..5228
                     /locus_tag="I9760_001134"
     CDS             4719..5228
                     /locus_tag="I9760_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAT5042910.1"
                     /translation="MESNNTQQSIKIHSSVIVLHDLSKDSLILTRRSQMLKKHPGEVC
                     FPGGFQEINDQDLYSTALRELNEELGVTSDRITLIRKLNIEKTLLGLVIHPWYASIDS
                     IFPYTMNPQEVSELILVPMPLVKNQKNYKEIRIEKEGKHIVTCQFIANEELIWGATAR
                     IMRQLASQY"
     gene            5382..6119
                     /locus_tag="I9760_001135"
     CDS             5382..6119
                     /locus_tag="I9760_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAT5042911.1"
                     /translation="MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYG
                     FDIDLMQEICRRLHATCTFEAYIFDDLFPALKNREVDLVIASMIITDERKKHFIFSLP
                     YMESNSQYITTVDSKISTFDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDML
                     LGLSNNQVDASLMDYEAAKYWMASEPYAYKLIGKKYKLIGKKISIGEGYSIMANPDQF
                     VLIKKINKILLEMEADGTYLRLYSEYF"
     gene            complement(6167..7876)
                     /locus_tag="I9760_001136"
     CDS             complement(6167..7876)
                     /locus_tag="I9760_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HAT5042912.1"
                     /translation="MDKRWFEQYQNGVPHEIDASQYLSLISLFNESCSSYASRAAYSN
                     LGTEITYAELDSLSRNFAAYLQQLNLEKGARVAIMLPNVLQYPVAIFGVLRAGYVVVN
                     TNPLYTADEVIHQMNDSGAEVIIVLANFAKTVEKALPSMPSVKHVIVTQVGDLFPSFK
                     RFIVNFVVNHIKKLVPAFTIPHAVAFNYALSEGKQATLHRVELNHDDIAFLQYTGGTT
                     GVAKGAILTHGNLIANVMQAYTWISPLGVSNQDVIVTALPLYHIFSLTANCLTFMKIG
                     AKNILITNPRDMGHFIDQIKNSGFTAITGVNTLFNALLNHPKFKEIDFSKLKLALSGG
                     MALQKSVSLKWSEMTKTRVLEAYGLTETSPAVTINPMYLEGYNGSIGLPLPSTDVSIR
                     DESGKEVPIGTSGELCIKGPQVMPGYWKRPDETALVFTKDGFLKTGDIARMDEEGFIY
                     LVDRKKDMIVVSGFNVYPNEVEQIIGMHPGVLEVGVVGIVDEESGERVKACIVRKDST
                     LTAEQIINHCKEHLTAYKIPKVVEFFDELPKTNVGKILRRALKNQEKEQKSSANKKTM
                     EPV"
     gene            7948..8643
                     /gene="minC"
                     /locus_tag="I9760_001137"
     CDS             7948..8643
                     /gene="minC"
                     /locus_tag="I9760_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAT5042913.1"
                     /translation="MGENITNTQAFKLKGRLYTLTVLQVLNTDKNAFSQQFAEIVIRA
                     PKLFERTPVVFDLSSVNHIEFDLQELCQIARNHGMIPLAIQGGSPLHDTLAQCQGLGI
                     LNASTAHDKPIIEESSECQSIENFKTKLLTTPVRSGQQVVAKGADLIVTSSVSHGAEL
                     LADGNIHVYGTLRGRALAGISGDIEARIFCQSLDAELVSIAGFYRISDAIEPVNGPCQ
                     IYLANEHIVVEPL"
     gene            8640..9368
                     /locus_tag="I9760_001138"
     CDS             8640..9368
                     /locus_tag="I9760_001138"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAT5042914.1"
                     /translation="MIDSVFISDLHLHPEDNEIQTRFDHFVNWAQLSVKRIYILGDFF
                     HAWSGDDSINAWSTAIAEQIESLTQSGIEIYFMTGNRDFLLGSLFAKRTGWKILTEPT
                     IITLGNHKILLVHGDRYCTNDRSHQRFRLLTRNRWFSFLFLRLPLSFRKRLVNGVRKI
                     SLKNHHKSIEQMDIVTEAAIRHMSENNTEILIHGHTHKPGITSYNYEEKILTRYVLSD
                     WDDIPKLLCYDNTKGFYFTQYDIN"
     gene            9365..10111
                     /gene="ravY"
                     /locus_tag="I9760_001139"
     CDS             9365..10111
                     /gene="ravY"
                     /locus_tag="I9760_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavY"
                     /protein_id="HAT5042915.1"
                     /translation="MRSYPMSKPSNKMKEELEQIRKTEIENAIAKEREDLLAQRSRER
                     AEADDNLKKGRYIKDPRTGQSITLWESAVQKAEEVLNADLNSYMDARAAIMSLLKMYY
                     EMVKANAQTMKELRAGIGNTIMDWGVFPIKDYIGKKLTGNPEIDLPILQHEVSYTDDN
                     KLKIEPLTRSDKHEEKGQLDKLFEGIIHMWLKNNDYTPDRNNPGQFVNSHGETLDKAT
                     FDKLKKDDQHGLNHFLTEYDPDLQFRPSRP"
     gene            complement(10200..10823)
                     /locus_tag="I9760_001140"
     CDS             complement(10200..10823)
                     /locus_tag="I9760_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HAT5042916.1"
                     /translation="MLKRLDSDLQTLNDFLKTKTPEAWLNHAAANIPLLLLDHAHCER
                     KAAGTAINFISKYPEKAELVAIMAPLAREELLHFEKVIDIMKQKGIVYSPLQPSDYAS
                     NLHKHVTNKDGIERLCDQLIIGAIIEARSCERFSSIIPYIEDQDLARFYKTLVKSECR
                     HFEEYLHLAKFYGGSVDDRISEFLTIENAFILSDDVVFRFHSGLPPA"
     gene            10897..11781
                     /locus_tag="I9760_001141"
     CDS             10897..11781
                     /locus_tag="I9760_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042917.1"
                     /translation="MDEIKKDDELSQWLSTYGTITAERILGRYNISLPQDEILEAINI
                     PSSFYRHLLQIPLKNVLNGIVIQQASDYHVYAQKLLIDYLLSGESSKEPDSQGAGTRE
                     SLEDERQRLVQLGDEFHKLELEQDNLIASSQASLMKISIDWNTKLETTLSKLNSLYKN
                     TNSKIKKNAIRKALIKAFIHCDLVKDQSQKNKYQLIDKLNQTLAVSVGAELKESILTN
                     LSELFQILEALNTKLDEFTDRTNHLSQQAKSFRTQFYEVILRIIELIKLLPEYKIDPA
                     QDAINREPLYFDRTIGER"
     gene            11916..12341
                     /gene="ndk"
                     /locus_tag="I9760_001142"
     CDS             11916..12341
                     /gene="ndk"
                     /locus_tag="I9760_001142"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAT5042918.1"
                     /translation="MAKELTLSIIKPDAVAKSVIGEIYTRFEKAGLDIVAAKMTQLSR
                     EQAESFYDIHRARPFFKDLVDFMISGPVMIQVLKGENAVAKNREIMGATNPKEAAPGT
                     IRADFADSIDANAVHGSDSLENAAREIAFFFEQHELCNR"
     gene            12362..13510
                     /locus_tag="I9760_001143"
     CDS             12362..13510
                     /locus_tag="I9760_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAT5042919.1"
                     /translation="MDQQKVNLLNYNYSQLRELLIAWDEKPFRAQQLFQWIHQVGIRD
                     FAQMTNLGKVLRNKLSQLACIDLPEIVACQKSADGTHKWLLKLECGNCIETVFIPEAN
                     RGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGTHDKKIT
                     NVVMMGMGEPLLNFDNVVSAMNIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVA
                     LAVSLHAPTDELRNELVPINKKYPLSQLISLCKRYFKDEPRRKVTFEYVMLKGVNDQP
                     EHASQLIKLLHNVPAKVNLIPFNPFPLTQYQRSSRETIDAFRDKLMKHGINTITRKTR
                     GDDIDAACGQLAGEVKDKTSRSQRWQKLHFMSKTDKSTELTISSEEIA"
     gene            13690..14472
                     /gene="pilW"
                     /locus_tag="I9760_001144"
     CDS             13690..14472
                     /gene="pilW"
                     /locus_tag="I9760_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HAT5042920.1"
                     /translation="MLKSIRYLLTIIICLFVGACQHTQDNEEPNSIRKVNLSKAASFN
                     VQLGLGYLKQGDRPRAKKKLLTALEQQPDSADVNAALAYYFEQTKELEQAKKFYHKAI
                     TLSQNGGAQLNNYGAFLCRQGDYKNAETYFLKAVKDQNYVHTSGAYENAGLCAMAIPD
                     HDKAILYFTKALNQDPSRKESLYELVKLQSKIGHDKEALELLQKHADLVLNDKVMLAL
                     AKDIANRTGQYTLAAEYENSFNKLEQTNNISGVNDEYNSSNG"
     gene            14447..15016
                     /locus_tag="I9760_001145"
     CDS             14447..15016
                     /locus_tag="I9760_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT5042921.1"
                     /translation="MNTIAAMDETRNNEFTNPGYELAALRQQKGYSIEYVASKLHLRA
                     RIIELIEAGDFDLLPQPVFVKGYLRAYSKLLGVSPEPFLTLFNAQYCFEKKPERAALW
                     QSKKESHKAEHFIKWFTILFAIGVLVAVGIWWQKNKDSQSIDTPKENSSGLSISETSS
                     EIKLTDLSKMESLLTPNNTQMTPLEKNGG"
     gene            15006..16286
                     /gene="hisS"
                     /locus_tag="I9760_001146"
     CDS             15006..16286
                     /gene="hisS"
                     /locus_tag="I9760_001146"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAT5042922.1"
                     /translation="MVVDKIQAIRGMNDVLPDSTSIWRFIEQTFINCLVRYGYKEIRF
                     PIVENTQLFKRTIGEITDIVEKEMYTFNDLNGDSITLRPEGTAGCVRACIEHGLLHNQ
                     QQKLWYLGPMFRHERPQKGRYRQFNQFGVEALGITGTGIELELISICRRLWIDLGFSQ
                     SVQLQVNSLGEINERQKYRSILVEYLRDHFQILDEDSKRRLDKNPLRVLDSKNPDLQQ
                     LIQNAPKLIDVLGDDSREHFQSFCNGLETLGIPYSINPVLVRGLDYYGQTVFEWVTDQ
                     LGSQATICAGGRYDMLVEFLGGAPTPAVGFALGLERIFLLMETLNLLNESNNKQSIFI
                     IATNEEAILKALVMAESIRNAHPSLDVITNTAGGGFKSQFKKADKSGARLALILGEDE
                     IAKEYVSIKDLRTEIEQISIPMTKINEFLQDYLA"
     gene            16304..16978
                     /locus_tag="I9760_001147"
     CDS             16304..16978
                     /locus_tag="I9760_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAT5042923.1"
                     /translation="MSVYMTEEEQLEIIKKWWKRYGNMITIFLSIMLLIIAGYRYMNW
                     HNDKIKQQASIAYENMMIAFSNQNIKSVRSYANELIKNYNHTVYADIAHMTLAKIYVN
                     KNKLEQARNELKAVASESQMTPLRQIAKIRIARLLAADKAYTNALNELSAVEDEAYLP
                     VINELKGDIYSAKGQYQDAMNAYRLAIDEVRTNGMGNLFLEMKTNELAIKTQSMITDD
                     KKVKAA"
     gene            17003..18157
                     /gene="bamB"
                     /locus_tag="I9760_001148"
     CDS             17003..18157
                     /gene="bamB"
                     /locus_tag="I9760_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAT5042924.1"
                     /translation="MKIRILVLILCALTQGCTYVDDYMLGKDNTPQPKELKEIQPKVK
                     MAQSWTTPVGKAHKTNEYLNIKPAIRGDIIYTADASGLVQAVNRKDGQIKWSTALKNN
                     IVSGPTVAAGYVAVGTNASTLVLLNQSDGKEIWQNKVSAEVLAPPAISHQKVIAKTID
                     GKVYAIDAVNGKQLWVADHGAPSLVLKASSSPIIVDNLVLVGFSDGKLDALELQTGRL
                     IWQRSIAYGTGASDVERLVDIDSDPIISNNVAYLATYQGYVGALSLSDGQFIWRKPAS
                     VYKNMLLSHNNLYFTDSNDVLWSLNSSTGQVNWKQTSLKARGLTAPALVGGNLAVGDK
                     TGYLHILSTQTGELLGRSQLSGGVTVSPSVSGKNMYVLTNNGMLNQLSVS"
     gene            18162..19550
                     /gene="der"
                     /locus_tag="I9760_001149"
     CDS             18162..19550
                     /gene="der"
                     /locus_tag="I9760_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAT5042925.1"
                     /translation="MIPVIALVGRPNVGKSTLFNRITKTQDALVADFPGLTRDRQYGH
                     AQHENKSFIIVDTGGIGVDDIEVDTLMSRQSQVALNEANVILFLVDGRSGLTGIDQQI
                     AQALRKFNKKVHLVVNKTDGMNEDIACADFQSLGITDIHAISASHGGGISSLLEEILE
                     PFTTETHEATDDKAIKIAFAGRPNVGKSTLINRILGEERVVVYDMPGTTRDSISIPFT
                     REDKQYVLIDTAGVRRKSRIDEKIEKFSVIKTLQAIKEAHVCLLLLDANEGITDQDMN
                     LLGFIIESGKALVIAVNKWDGLEEDHKEKIKSELSRRLHFANFAKIRFISALHGSGVG
                     GLFKDINEAYHSAIQSFSTPKLTRLLQDISAKHTPPCINGRRIKLRYAHLGGHNPPVI
                     VIHGNQLDALPESYKRYLNNEFIKHLGLVGTPLKIEFKGGQNPFANKKNKLSQRQVNK
                     KKRLMRWAKSKK"
     gene            complement(19615..20037)
                     /locus_tag="I9760_001150"
     CDS             complement(19615..20037)
                     /locus_tag="I9760_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAT5042926.1"
                     /translation="MYTNILFATDLLNEHSHLTEKAANIAKQFNAKLYLLHVIELPTS
                     ILIAQGLGFTELANPSKEDAQTVLSLIGESLQIPQEQQFVEIGSVKEHILNKAKDLNC
                     QLIIIGSHSATGLPSILGSTAHAVVNHSVCDVLTLKAD"
     gene            complement(20092..20517)
                     /gene="folK"
                     /locus_tag="I9760_001151"
     CDS             complement(20092..20517)
                     /gene="folK"
                     /locus_tag="I9760_001151"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAT5042927.1"
                     /translation="MNVCYLSLGSNQKNPERQIRQAINEIKQIPSTCLIKVSRLYWNK
                     AWGFENQQEFCNVVIEIRTTLLPHKLLKWCQKIENRHKRVRRKFWGPRTLDIDIILYG
                     YRLIRTKNLIVPHPCFHLRDFVLVPLRELNSNLKIPNYT"
     gene            complement(20514..21824)
                     /gene="pcnB"
                     /locus_tag="I9760_001152"
     CDS             complement(20514..21824)
                     /gene="pcnB"
                     /locus_tag="I9760_001152"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAT5042928.1"
                     /translation="MLRRNRSHGHAIDLAYIIPRNKHNISKADISNNALKVLNRLISH
                     GFQAYIVGGSVRDLLLHKAPKDFDIATNATPNEVKNLFKNARIIGRRFKLVHILFHRE
                     IIEVATFRGHNSSDASHQFNERGMLVRDNVYGSLDEDAWRRDFTINSLYYNISDSSII
                     DFTGGVEDVKQKLIRIIGDPVTRYQEDPVRMLRAVRFSAKLQFNLSPETKAPFPKISH
                     LITHVSSSRLFDEITKLYQCGEAESVQKLLVEYGLFNYLFPQTAALFDSHYPVNPLLG
                     IALENTDTRIRDGKPVTPAFLIAVLLWFPMIECSRKLQESGLPPLPSIEKAMSIVLSE
                     QNAIISIPKRYSQVIREIWLLQYRFEKRFGGRAFNLLLHPRFRAAYDFMALRALSGDE
                     SMELAQWWATFQEVDNVEKNNMVTQLAATTPGRPKKRRKRKIAK"
BASE COUNT         7251 a         4015 c         4133 g         6638 t
ORIGIN      
        1 gaaaagacag atagttgtag taatagtatt tgttgttagg cctcgctatt tacgaagccc
       61 aacataaatt aaaatcatgc ttgttgcaaa gattcaataa cagcacctaa aaatcgagtg
      121 gcttctcctc cggtaatagc tctatgatca aagctcaagg ataaaggaag cattttgtgg
      181 gcttcaattt tatcctcatc attaattact gcggctttat aaagtcttcc aactgctaaa
      241 atcgctacca ttggaggaac aattattgga ctggcaaaac gtcctgaaaa ttttccaaaa
      301 ttagacaacg taattgtcgc cccttttagc ttgtctgcac taaccgcccg attacgaact
      361 gactctttga attcatcaat tatttttctt aattcactgt cggagtattt ttctgcatca
      421 tggataacag gaacaaacaa accttcctca ttgtccatgg caatgccaag atgcacttct
      481 ttaaagcatc tacgtgcggc atgtttggta tcaaaccagg cattaagagc aggttccttt
      541 tttgaggcat ggataatagc ccggattaaa cgaacagtaa tgtctgtgtt gggtttccaa
      601 atatggatat ctgcttcatc aaaaatactg actggaacta tttctgcatg agattgtacc
      661 atgctattaa gcatggctcg tctaacacct cgcaagtgct caaacccggc aggtggttgt
      721 gaattgatat tggcctgatt ttgaacatct tctcgagtaa taacaccatt gtcaccacta
      781 ccttttaaag agcttaaatc aactcctaat ttttttgcta acaatcttac agcaggtgtt
      841 gtcttaacgc ggtgagaaga acgctgactt ccaataataa aattatcttc tgacacatca
      901 gtactttcct ccagattacc aacaactgtg cccttatctg caggtttttc ggtagttgaa
      961 acaaacgcta caagaggctc acctgttttg ataacatcac ctggtttgcc ataaagtttt
     1021 gcgatagtgc ctgattgagg gcagggtacg tcaacaactg cttttgccgt ttccatagaa
     1081 actaaaggtt gatctgcttt aactgtgtcg ccttccttaa caaaccattc atgaatttca
     1141 gcgtcaggta aaccttcacc taaatcgggt agattaaaaa tattcattat tactccatta
     1201 tactcataac agtgttttta atacgtgcaa tgctaggtat gtactgtttt tcaagttgga
     1261 aataaggcat aaccgtatca tatcccgtaa ctctttgcac aggagctagt aaatctgcca
     1321 tacaatgttc cataatttga gccgatattt cagcacccac tccacaagtt tttgctcctt
     1381 catgcactat gacgcaacgt cctgtttttt ctacagaact taaaatagtt tctatatcca
     1441 gaggcttgat tgttgctaca tcaataacat cacaggaaat accttcatca gttaattgtt
     1501 tggctgcctg taaggtttca tgcatactgg ctccccagct aattaatgtc agatcatctc
     1561 cctgttgcag ggtaaaacat ttacctaacg gtaaggcttg tccatcatct ggaacaggtt
     1621 gtttgaccaa gcgataaatt cgttttggtt caagaaagat aacgggatca gggttacgta
     1681 tagcggccaa caacagacca taagcacgtt ttggagaaga gggaataacc acttgcaaac
     1741 ccggaatatg agcaaataat gcttctgtac tttcagaatg atgctccggt gctctgattc
     1801 caccaccaaa cggagcacga taaaccaaag ggcaatgtaa gcgacctctg gttctgttac
     1861 gcatacgcgc tgcatgagat atgatctgat tcatggctgg ataaataaaa cccatgaatt
     1921 gaaattcagc aacaggtttt aagccttgaa tggacattcc aacagccagg cctgctatca
     1981 tcgattctgc taatggagta tcaaaaactc tattttctcc gaatctgtct tgtaaaccta
     2041 cagtagctcg aaatactcca ccatttttac caacatcttc gccaaagact actacatttt
     2101 catcatgagc taactcatac gccaaagcct gagtaacagc ttcaactaaa gtaatatcag
     2161 gcatgagaat actcctccat ggctattgcg cgttgttcaa ttaaatattc tggtaattct
     2221 gcgtaatgat aatcaaaaat gctgctaaca ggctgaggtt tggtattcaa atattcctct
     2281 actgcttttt ccaccgtttc actgcattct ataaccagct tttcttcatc ctgagatgtc
     2341 caaatatttt gttgcattaa atagtgttta aaacgaacaa taggctcttt ggcttttgcc
     2401 tcctctactt catgagaagg ctgatagcgt gttgcatcat cagccgttgt atggtcgcac
     2461 aagcgatagg ttaaggcttc gattagagta ggtccaccac cttgacgagc cttttcaata
     2521 gcatccccta taacctgtct tgcagcaaaa atatcattac cgtctatttg aacgccagaa
     2581 aaaccagcag caatcgcttt ttgggctatt gtttgtgagc cagtttgttt ctctataggc
     2641 acagaaatcg cccatttgtt attatttact acgaagacca ctggtaattt ccatgcccca
     2701 gccacattca tggcttcgta aaaatcacct tctgaagtgc ctccttctcc aatacatacc
     2761 acagctactc ttggttggtt tctgtatttg aaggcaaatg cgactcctgc ggcatgcaaa
     2821 cattgagatg caattgggac acaaataggc aaatcttcgg cattattaga ataatgactt
     2881 cctcgctcat cccctcccca aaaggcgagt atctctgaca ttttaacgcc tctctgaaat
     2941 tgagcggcat aatctctgta ataaggtaca agtacatctt caggtttaag agcatgccca
     3001 attgcagtcg aaatagcttc ctggccatta attggcgcat aggttcccat tttccctgtt
     3061 ctttgtaatg caattgcttt tttatcaaaa gtacgggtga gtaccatgat tttatacaat
     3121 tctttaagta tggattgatt atctgcgaat ggaggtaatt cagcgaccaa cttaccttgt
     3181 tcattcaggt attgtgtgta tgttatttca aattgagcaa cagttgtcat tacatggctc
     3241 cttgtcaaca acgcgttggc ataggatact cacaaattaa tttaaaaacc agtaaattaa
     3301 taggaaccca agataaaaat atacgttttt tattgataag ttaggttaaa atgaccaaat
     3361 gaaaaacact gcattttttc acagcacatc cttatcttga ggtgataatt tgattgatag
     3421 tactcttatt acacttgact accatgattt ttcatctgaa tcctatgaaa aattcagtcg
     3481 tttacctgga tttgtcctgc ttcagagtac agacaagtca acggggcgtt atgatatttt
     3541 aagtgcttat ccatatgaga ggatggttat tgagaataat gtacatgata aagatttagt
     3601 attaacagaa ttaaaaaata aactaaaaat agaggagtca gaatttgatc ttccttttca
     3661 gggcggggcc ataggctacg tttcttacga ctttggttca ctcttacata atcatgaaca
     3721 atattcacaa aaagatctgg cgcatatacc tgttttaaat ttcggatttt atgattgggc
     3781 tatcatagtt gatcaccatt taaaaaaggc cactttattt gcgagaaata gccattcttc
     3841 aacaaaaagc gttgtagata aaataaagaa tatattgaat gatcaaccca tgaaatcggt
     3901 acaattttca gttcaagctg aatttttgcc tttaatatca aaagagaact acaggagctc
     3961 attcaatgcc attcaacaag ccctgagagc aggacgctct tatcaggtta atttcacgca
     4021 accgtttcaa acaaaattca ccggggatct ctgggatatg taccaaaggg ttaacaaaaa
     4081 taaccctgtc ccctatgctg cttttatcag aactgaggaa gctgacatat tgagcttttc
     4141 tcctgagcga tttttacttt atgacaacgg tgaaatgtta acttcgccca taaaaggcac
     4201 tatccaccga tcgaaagatc ctgttgtaga taaaaaatta aaagaacagt tattgaaatg
     4261 ccctaaaaac aaggctgaaa atgtcatgat tgtcgatttg ctaagaaacg atctagggaa
     4321 acttgccaag gctggcagtg taaaggtaat gaatctttgc cacatccaga gcttcaatgc
     4381 agtacatcat ttggtttccg acataagagc tctatgcaaa gaggatattc atccaattga
     4441 agcatttttc tcctgttttc caggtggttc aattaccggg gcgcctaaat tagagacgat
     4501 gaaaattatc agtgaacagg aaacctataa aagaggggtc tattgcggtt gcatagccta
     4561 tttttcacaa catggccgtt tcgatgccaa tatcgcgata cgtacggtga cagccaaaga
     4621 taatgtttta tatttggcag caggaggggg aatagttatt gactccgatt gggaagatga
     4681 atatcgtgaa tgtttcacaa aaattactgc aattatcaat ggaatcaaat aatacccaac
     4741 agtcaatcaa aatacattca tcagttatcg ttttacatga tctttccaaa gactcgttaa
     4801 ttctcaccag gcgaagtcag atgcttaaaa aacatccagg agaagtgtgc tttcctggtg
     4861 gttttcaaga aataaatgat caagatctat atagcactgc attgagagaa cttaatgaag
     4921 agctaggtgt tacatcagac agaataactc tgatcagaaa attaaacatt gaaaagactt
     4981 tactgggttt agtcattcac ccttggtacg ccagtattga ttctatattt ccttacacta
     5041 tgaatccaca agaggttagt gaattaattc ttgtgcctat gcctttagta aaaaatcaga
     5101 aaaattataa agaaattaga attgagaaag agggaaaaca cattgtcacc tgccaattta
     5161 ttgctaatga agaattaata tggggtgcca cagcaagaat tatgaggcaa ttagcttctc
     5221 aatattaagt attataccag gatgataaaa ggcatttctt taaatgcctt tacctgatta
     5281 taaaccaaat tagagaaatc atatgctaca aattgtacaa atcaaatttt ttgtaccatg
     5341 attatgttta aacaaacttc ctaaattaca ggatatagtt tatgaagtta ctacaacttc
     5401 tcttatttat tattatttcc tctaattctt attcattaaa tttaacaatt ggtacttcaa
     5461 aatttaatcc gccttttgaa gtttggtctg gtaacaacag ttcattgtat ggctttgata
     5521 ttgaccttat gcaagaaata tgtagaagat tacatgcgac ctgtactttt gaagcctata
     5581 tattcgatga tttatttccc gcgttaaaaa accgcgaagt cgatctggtt attgcctcaa
     5641 tgattattac agatgaaaga aagaaacact ttatttttag tcttccctat atggaaagta
     5701 actcacaata tataactacc gtcgattcta aaatcagcac atttgatgat ttgcacggaa
     5761 aaaaaattgg agtaagaaaa ggaactcctt acgcaaggca agttttgtca gaaaaccgca
     5821 acaatcaggt aattttttat gagctcatac aagatatgct tcttggctta agcaataatc
     5881 aagttgatgc cagtttaatg gattatgaag cagcaaaata ttggatggca tctgaaccct
     5941 atgcttataa attaattggt aagaaatata aattaattgg taagaaaatt tctattggag
     6001 aagggtattc tattatggca aaccctgatc aatttgtatt aattaaaaaa ataaataaaa
     6061 ttcttcttga aatggaagct gatggaactt atctgagact atattcagag tacttttaga
     6121 agaggagtat taaggacaaa tagcacccaa aatactccaa ttatacctaa acaggctcca
     6181 ttgttttttt attggccgag cttttttgct ccttttcttg gttttttaaa gcacgacgta
     6241 aaatcttgcc cacatttgtt ttaggaagtt cgtcaaagaa ttcgacaact ttggggattt
     6301 tataagcagt caaatgctcc ttgcaatgat tgataatttg ttcagccgtc aatgtactat
     6361 cttttcttac aatacaggct ttaacccgtt caccagattc ttcatcaaca atacctacaa
     6421 cgcctacttc taatacaccg ggatgcattc ctattatttg ctctacctca tttggataaa
     6481 cgttgaatcc tgaaacaaca atcatgtctt tttttctatc gaccaggtag ataaagcctt
     6541 cttcatccat acgtgcaata tcgccagttt ttaagaaccc gtctttagta aataccaagg
     6601 ctgtctcatc aggccttttc caataaccag gcatcacttg tggtccttta atgcataact
     6661 ccccacttgt accaataggc acttctttcc cggattcatc acgaatagat acatcagttg
     6721 agggaagagg taagccaata ctgccattat agccttcaag gtacatcggg tttatagtta
     6781 cagcagggct agtttcggtt aatccataag cttccaaaac acgtgtttta gtcatttcag
     6841 accattttaa ggacacactc ttttgcaatg ccataccgcc tgataatgcc agttttaatt
     6901 tgctaaaatc aatttcttta aatttaggat ggttcagcaa tgcattgaat agagtattaa
     6961 ctccagttat tgctgtaaaa cccgaatttt tgatttgatc tataaagtgt cccatatcac
     7021 gtggattggt gatcagaata tttttcgcgc caattttcat aaaagtgagg caattggccg
     7081 tcaaggaaaa aatatggtac aaaggaagtg cagttactat aacatcttga ttgctgactc
     7141 ccagaggaga tatccaggtg tatgcttgca tcacattggc tataagattg ccatgagtca
     7201 aaatagcgcc ttttgcaaca ccagtagtac caccagtata ttgtagaaac gcaatatcat
     7261 catgattcag ttcaactcga tggagcgttg cctgtttccc ttcagataaa gcatagttaa
     7321 aagccacggc gtgaggaatt gtgaaggcag gaactaattt ttttatatga ttcactacaa
     7381 aattaacaat gaatctcttg aatgaaggaa ataaatcacc cacttgcgta acaatcacat
     7441 gcttaacgga aggcattgac ggcaatgctt tttcaaccgt ttttgcaaaa ttagctagca
     7501 cgataataac ctctgccccc gagtcattca tttggtgtat tacttcatcc gctgtgtata
     7561 aagggttagt gtttaccacg acataacctg cacgtaacac accaaagatg gctacaggat
     7621 attgtaaaac gttaggcagc attattgcta ctcttgcccc tttttccaaa ttcaattgct
     7681 gcagataagc agcgaaattt ctgcttaaac tgtccagttc agcataagta atttccgtac
     7741 ctaaattaga ataggcagct ctgctagcat aagagctaca tgattcatta aataaagata
     7801 tgagggataa atattgactt gcgtcaattt catgtggtac tccattttgg tattgttcaa
     7861 accatcgttt atccactttg atatccttct gttattgttt tcctcatgct agtataaaca
     7921 aaaaactctc taatggatat catgctaatg ggtgaaaata taactaatac acaagcattt
     7981 aaacttaagg gaaggttgta tactttaacc gttttgcaag ttttgaacac tgataaaaat
     8041 gctttttctc aacaatttgc tgaaatagtt attagagcgc ctaaattatt tgaaagaacg
     8101 cccgttgttt ttgacttgtc ttctgtcaat catatcgaat ttgatttaca ggaattatgt
     8161 caaattgcac gtaatcatgg tatgattcct ttggcaattc aaggtggtag tcctttacat
     8221 gatactctgg ctcaatgtca gggtttgggc atactaaatg cttcaacagc acatgataag
     8281 ccaataatcg aagaatcctc agaatgtcaa agtatagaga atttcaaaac aaaattacta
     8341 acgacaccag tgcgttcagg ccaacaagtc gttgccaagg gagctgattt gatagtgact
     8401 tcttcagtta gccatggggc agaattatta gctgatggaa atattcatgt gtatggaaca
     8461 ttacgcggca gagcattagc aggaatatcc ggggatatcg aggcaagaat tttttgccag
     8521 tctttggatg ctgaattagt atccatagcg ggtttctatc gaataagtga tgctattgaa
     8581 ccagttaatg ggccttgtca gatttacttg gcaaatgaac atatcgttgt tgaaccatta
     8641 tgattgactc cgtttttata tccgatttac atctgcatcc agaagataat gaaattcaaa
     8701 ccagatttga tcattttgta aattgggctc agctatctgt aaaaaggata tacatactag
     8761 gtgatttttt tcatgcctgg tcaggtgatg attcgattaa cgcatggagt actgctattg
     8821 cagaacagat agaatcattg actcaaagtg gcattgaaat ttattttatg actggaaatc
     8881 gtgatttttt gcttggctca ttatttgcca agcgaacggg ttggaaaata ttaacagagc
     8941 caaccattat tacacttgga aatcacaaga tcttactggt tcatggcgat cgttattgta
     9001 ctaatgacag atcacatcaa cgattccgat tattaacgag aaataggtgg ttctcatttc
     9061 tttttttaag acttccttta tcatttcgca aacgattggt taacggagtt cgcaagatca
     9121 gtctaaagaa tcaccataaa tcaatcgaac agatggatat tgttacagag gctgcaattc
     9181 gacacatgtc tgaaaataat acagaaatac tgattcatgg acacacacat aagcctggca
     9241 taacaagtta caattatgaa gaaaaaatac tgacaaggta tgttctaagt gattgggatg
     9301 acatacccaa actattgtgt tatgataata caaaaggatt ctatttcacc caatatgata
     9361 taaattgagg agttacccaa tgtctaaacc atcaaataaa atgaaagagg aactcgaaca
     9421 aattcgaaaa actgaaatag aaaatgctat agcaaaagag cgtgaagatt tacttgctca
     9481 aagaagccga gaacgcgctg aggccgatga caacctaaaa aaaggccgat atatcaaaga
     9541 tcctcgtaca ggccaatcaa taactctctg ggaaagtgca gtgcaaaaag cagaggaagt
     9601 tttgaatgct gatttaaatt cttatatgga tgctcgtgct gcgatcatga gtttattaaa
     9661 aatgtattat gaaatggtca aggcaaatgc tcagactatg aaggaattgc gcgcaggaat
     9721 tggtaacacg attatggatt ggggtgtctt tcccattaaa gattatattg ggaaaaaact
     9781 cacgggaaat cctgaaattg atttaccaat tttgcaacat gaagtgagtt acactgatga
     9841 caataagttg aaaattgaac ctctgactcg ctcagacaaa catgaggaaa aggggcaact
     9901 tgataaatta tttgagggta taatccacat gtggctaaag aataatgatt atacccctga
     9961 ccgcaataat cctggtcaat ttgttaattc acacggtgaa accttagaca aagcaacgtt
    10021 tgataaatta aaaaaagacg atcagcatgg acttaatcac tttttaacgg aatatgatcc
    10081 tgatttgcaa ttcagaccaa gcagaccttg attaagcaag agaaatattg ggttgcacta
    10141 gcctgtatat aatacttatg ggctagtgct gaaaaatctg ttgactctgt tttaaggcat
    10201 caagccggag gcaagccact atgaaagcga aataccacat cgtctgataa gataaatgca
    10261 ttttctatag ttaaaaactc cgaaatacga tcatcaacag aaccgccata gaacttggcc
    10321 aaatgcaaat actcttcaaa atgcctgcat tctgatttaa ccaatgtttt ataaaacctg
    10381 gctaagtcct gatcttcaat atatggaatt attgagctaa aacgctcgca cgatcgggcc
    10441 tcaatgatag cacctataat taattgatcg catagtctct caattccatc tttatttgtg
    10501 acatgcttat gcaaattgga tgcatagtct gatggctgca agggagaata aacaattccc
    10561 ttttgcttca tgatatcaat gactttttca aagtgcagta attcttctct tgccaatggt
    10621 gccataattg ctactaattc tgctttttct ggatatttgc ttatgaaatt aattgctgtt
    10681 ccagctgctt tacgttcaca atgagcatga tcgagcaata aaagaggtat attggcagct
    10741 gcatggttta accaggcttc cggagtttta gtttttaaaa aatcatttaa cgtttgtaaa
    10801 tcactatcta atcgttttaa catgataaaa tacactataa tttcaagtaa atagccctta
    10861 tatcacatac tttaaagaat ttaaattgga taaaaaatgg acgagatcaa aaaagatgat
    10921 gaattgagtc aatggctttc tacctatgga acaatcaccg ctgaacgtat attaggtaga
    10981 tacaacatat cactgccaca agatgaaata ctagaagcga ttaacattcc atctagtttc
    11041 tatagacatt tgttacagat tcccctaaaa aacgtactta atggaattgt aattcaacag
    11101 gcaagtgatt accatgtcta tgcacagaaa ctgttaattg attatttgtt atctggtgaa
    11161 agcagcaaag agcctgactc acagggggcc ggaactcgcg aatcacttga agatgaaaga
    11221 cagagattgg ttcagttagg ggatgagttt cataaattgg aattagagca ggataatctc
    11281 attgcatcaa gccaagccag cttaatgaaa ataagcattg actggaatac aaaactggaa
    11341 acaactcttt ctaaattaaa tagcctatac aaaaatacaa attctaaaat taaaaaaaat
    11401 gcaattagaa aagcgttaat caaggccttt attcattgtg atctggtcaa ggatcaatca
    11461 caaaaaaaca agtaccagct aattgataaa ttgaatcaaa cattagctgt ttctgtaggt
    11521 gctgaattaa aagagtctat cctgaccaat ctgagtgaac tcttccaaat tctagaagct
    11581 ttgaatacaa agcttgatga atttactgat aggactaatc atctaagcca acaggctaaa
    11641 tcattcagaa cccaattcta tgaggtaata cttcgcatca ttgagttaat taaactatta
    11701 cccgaatata aaattgaccc tgcacaagat gcgattaacc gagaaccatt atactttgat
    11761 agaacgattg gagagcgcta acgcaaaacc ctattttctc taaaatcata ctataactat
    11821 tgactgaatg gttgaatgca tgaattttta ttagccagac cactatgaat tcatgtataa
    11881 taatgcactt taaattaatc tcattggagt gcatgatggc aaaagaatta acattatcta
    11941 ttattaaacc tgatgctgta gcaaagtctg tgataggtga aatatatacc cgctttgaaa
    12001 aagcaggctt ggatattgtt gccgctaaaa tgacacaact ttcacgcgaa caagccgaga
    12061 gcttttatga tattcatcgc gcgcgaccat tctttaagga tctggtggat tttatgattt
    12121 ctggtcctgt aatgatccaa gtattaaaag gtgaaaatgc tgtcgctaaa aacagagaaa
    12181 ttatgggcgc gacaaatccc aaagaagcag cacctggaac tatacgtgct gattttgctg
    12241 atagtattga tgcaaatgct gtgcatggtt ctgacagcct tgaaaatgct gctcgtgaaa
    12301 tagcattttt ctttgaacag catgagttgt gcaatagata aaggcaggct tgggagcggt
    12361 aatggaccaa caaaaagtga atctattaaa ttataattac tcacagctac gagaattatt
    12421 gatagcgtgg gatgaaaaac ctttcagagc tcaacagcta tttcaatgga ttcatcaagt
    12481 tggcatacgt gactttgctc agatgactaa tttagggaaa gttttaagaa ataaactttc
    12541 ccaattagct tgtatagatt tacctgaaat cgttgcatgt caaaaatcag ctgatggcac
    12601 acataagtgg ctattaaagc tggaatgtgg gaattgtatt gaaacagtgt tcattccgga
    12661 agcaaatcga gggacacttt gtgtttcatc acaagtaggt tgtgctttaa attgttcttt
    12721 ttgctccaca gcaaagcaag gatttaacag aaatttatca acagctgaaa taattggtca
    12781 agtatggctg gcagcacgcg aactatcaga taataatggc actcacgata aaaaaataac
    12841 taacgtcgtg atgatgggaa tgggtgaacc gttacttaat ttcgataatg ttgtttctgc
    12901 tatgaacatc atgatggatg atttagccta tggactatcc aaaagacggg ttacattaag
    12961 cacttcgggt gttttaccgg aaatggaacg actcagagaa gtcagtcctg tcgcgttggc
    13021 agtatcctta catgcaccaa cagatgaact acgtaacgag ctggtaccaa ttaataaaaa
    13081 atatcctctc tctcaattaa tatccctatg taaacgatat tttaaagacg aaccaagaag
    13141 aaaagttact tttgaatacg tcatgctaaa aggagttaat gatcaacctg aacatgctag
    13201 tcaattaatt aaacttttgc ataatgtgcc tgcaaaagtt aatttaattc cttttaaccc
    13261 atttccttta actcaatatc agagatcatc ccgggaaaca attgatgcct ttcgagataa
    13321 attaatgaaa catggtatta atactattac tcgcaaaact cgtggagatg atattgatgc
    13381 agcatgcggc caacttgctg gcgaggtaaa agataaaacg agcagatcac aacgatggca
    13441 aaaacttcat ttcatgagca aaacagacaa atcaactgaa ttgaccattt cttcggaaga
    13501 aattgcataa cacagtcttg tataataacc gagagccaat taagaacttt ctccagtctt
    13561 cctggttgga gattatgtat aataaaggtg ttttcacctg cagactcaca ttaaatagtg
    13621 acaaatcatt tttaatttgg acttaagtgg ttataatgcg aaatcatttc taacgtaaag
    13681 tgattaatcg tgctaaaaag cataaggtac ttattaacta taataatttg tttatttgtt
    13741 ggagcttgtc agcatacaca agataacgaa gagcctaata gcattagaaa agtaaacctt
    13801 agcaaagcgg cttcttttaa tgtgcaatta gggttgggat atttgaagca aggcgacagg
    13861 cctagagcaa aaaagaagct gttaaccgcc ttggaacaac aacccgattc agcggatgtt
    13921 aatgcagcat tagcctatta ttttgaacaa accaaagaat tggaacaagc gaaaaaattc
    13981 tatcataaag caataaccct atcgcaaaat ggtggtgcac aattgaataa ttacggcgct
    14041 tttctgtgtc gacaggggga ttataaaaat gctgaaactt acttcctgaa ggccgttaaa
    14101 gatcaaaact atgtgcatac ctcaggcgct tatgaaaatg ctggattatg tgctatggct
    14161 atacctgatc atgataaagc cattttatat tttacgaagg ccttaaatca ggatccatcc
    14221 agaaaagaat cactatatga attagtaaaa ttacaaagta aaataggaca tgataaagaa
    14281 gccctcgaac tgcttcaaaa acatgctgat ttagtattaa atgataaggt aatgcttgca
    14341 ttagcgaaag acatagcaaa tagaacaggc caatatactc ttgcagcaga atatgaaaat
    14401 agcttcaata aactggagca aactaataat attagtggag taaacgatga atacaatagc
    14461 agcaatggat gagacaagaa ataatgaatt taccaatccc ggctacgagc tggcggctct
    14521 tcgccagcaa aaagggtatt caatcgagta tgttgcgagc aaacttcatc tacgagctcg
    14581 tattatagag ctcattgaag caggagattt tgatttattg cctcagccag tttttgtaaa
    14641 agggtacttg cgcgcctatt caaaattatt aggagtttca cctgaacctt ttttaaccct
    14701 ttttaatgca caatattgtt ttgaaaaaaa accggaaaga gcagccctat ggcaaagtaa
    14761 aaaggagtct cataaagcag aacattttat aaaatggttc accattttat ttgcgattgg
    14821 agttttagtc gcagttggga tatggtggca aaaaaacaag gatagccagt ctatagatac
    14881 cccaaaagag aattcatctg gattatcaat cagtgaaacc agttcggaaa ttaaattgac
    14941 tgatttatca aaaatggaat cgttattaac gcccaacaat acacaaatga cacctttgga
    15001 gaaaaatggt ggttgataaa attcaagcta tccgaggaat gaatgatgta ctgcctgata
    15061 gtacctcgat atggcgcttt atagaacaga cttttataaa ttgcctcgta cgatatggat
    15121 acaaggaaat acgttttcca attgttgaga atacacagct atttaaacgt acaattggtg
    15181 aaataacaga tattgttgaa aaagaaatgt acacttttaa cgatttgaat ggtgatagca
    15241 taaccctaag gccagaaggc acagccggct gtgttcgtgc ctgtatagaa catggtttat
    15301 tacataatca acaacaaaaa ttatggtact taggccctat gtttcgtcat gaacgtcctc
    15361 agaagggtag ataccgtcaa tttaaccaat ttggggttga ggcgctagga attactggca
    15421 ctggcattga gcttgagctg atctcaattt gccgcagatt atggatcgac ctgggttttt
    15481 ctcagtcagt tcaattacaa gttaattcat taggtgaaat aaatgaacgt cagaaatatc
    15541 gctctatcct ggtagagtat ctacgcgatc attttcagat acttgatgaa gacagtaaga
    15601 ggcgattgga taaaaaccct ttgagagttc ttgatagtaa aaaccctgat cttcaacagc
    15661 tcattcaaaa tgcccctaag ttaattgatg ttcttggtga tgatagcaga gaacactttc
    15721 aatcgttctg caacggattg gaaacacttg gtattcctta ttccatcaac cctgtattgg
    15781 taagaggatt agattattat ggccaaacgg tgtttgaatg ggtaacagac caattaggaa
    15841 gtcaagctac aatatgtgca ggaggtagat atgatatgtt ggtggagttc cttggcggag
    15901 cacccacacc tgcagttggg tttgctttag gattggaaag aatatttctt ttaatggaaa
    15961 ctttaaactt gttgaatgaa agtaacaata aacaatcaat atttatcatt gctactaatg
    16021 aagaggccat attgaaagcg cttgtcatgg cagagtcaat tcgaaatgct catccatctc
    16081 ttgatgtcat taccaatacg gcaggcggtg gttttaaaag ccaatttaaa aaggccgata
    16141 aaagcggggc tcgcttggct ttgattcttg gggaagacga aatagccaag gaatatgtaa
    16201 gcattaaaga tttgagaact gaaatagaac aaatatcaat accgatgacc aaaataaatg
    16261 aatttttgca agattatcta gcttaagggc aggagaatat tatatgtctg tttacatgac
    16321 ggaagaagaa caattagaga ttataaaaaa atggtggaaa cgatatggca atatgataac
    16381 tattttttta tcaataatgc tcctgattat cgcagggtac cgatatatga attggcacaa
    16441 tgacaagata aaacaacaag catctattgc ctacgaaaat atgatgattg cattttccaa
    16501 tcaaaacata aagtctgttc gttcctatgc caatgaatta attaagaatt ataaccatac
    16561 tgtttatgct gatatagctc atatgacact tgcaaaaatc tacgtaaata aaaataagct
    16621 tgagcaagca agaaatgagt taaaggctgt tgcatcggaa agccaaatga caccgctaag
    16681 acaaattgcc aaaatccgca ttgcacgttt actggcggca gataaagctt ataccaatgc
    16741 attaaatgaa ttaagtgctg ttgaggacga agcctacttg ccagtaatta acgaactgaa
    16801 aggggatatt tatagcgcca aaggccaata tcaggacgca atgaatgcct accggcttgc
    16861 aatagacgaa gtaagaacaa atggaatggg taatttattt ttagaaatga aaacaaatga
    16921 gttagcaatt aagactcaat ctatgattac tgatgataaa aaggtaaaag cagcttaatt
    16981 ttattataaa ggtttgtttt ttatgaagat tagaatatta gttttaatac tatgtgcctt
    17041 aactcaagga tgcacatatg tggatgatta tatgctcggt aaggataata cgccccaacc
    17101 caaagagcta aaagaaattc agcccaaggt taaaatggca caaagctgga caactccagt
    17161 tggaaaggct cataaaacaa atgagtattt aaatatcaaa ccagctattc gtggcgatat
    17221 aatttatact gcagatgcaa gtggactggt tcaggcagtt aacaggaaag atggtcagat
    17281 aaagtggtct accgcattaa aaaataatat agttagtgga ccaactgtag ctgctggtta
    17341 tgttgctgta ggaactaatg cctccacatt ggtactactt aatcaatcgg acggaaaaga
    17401 gatatggcag aataaagtat ctgctgaagt attagctcct cctgctatct cacatcaaaa
    17461 ggtcattgca aaaaccattg atggtaaagt gtatgcgatt gatgcggtaa acggcaagca
    17521 actgtgggtt gcagatcatg gcgctcccag cctggtgtta aaagccagct cgtcccctat
    17581 cattgtcgac aatctggttc ttgtcggatt ttccgatggc aaattagatg ctttggaatt
    17641 acagaccgga cgattgatct ggcaaagaag cattgcttat ggaacaggag ctagtgatgt
    17701 cgaacgttta gttgatattg attctgaccc tataataagc aataacgtcg cctatttggc
    17761 aacttatcag ggatatgttg gagcactatc actgtctgat ggccaattca tatggagaaa
    17821 gccagcatct gtttataaaa atatgctttt aagtcataat aatctgtatt ttacggacag
    17881 caatgatgtc ttgtggtctt tgaatagtag cacaggacag gtcaactgga agcaaacctc
    17941 cctaaaggca agaggtctta ctgcacctgc attagttgga ggaaatttag ctgttggtga
    18001 taaaacagga tatttacata tcctatcaac tcaaacaggt gagttacttg gacgttctca
    18061 gctttctggt ggagtaacag tatcaccgag tgtttctggt aaaaatatgt atgtattaac
    18121 caataatgga atgctcaatc aactttcagt gagctaataa aatgatccct gttatcgctt
    18181 tagttgggcg tcccaatgtt ggaaagtcta ctttgtttaa tagaattact aaaacgcagg
    18241 atgctcttgt cgcagatttt cctggcttga caagggatag acaatatgga cacgctcaac
    18301 atgaaaacaa gtcctttatt attgttgata ccggaggcat aggagttgat gacattgaag
    18361 tagatacttt gatgtcaaga cagtctcaag ttgctttaaa cgaagcaaat gtgattcttt
    18421 tcttggtgga tggccgttca ggactaacag gtatagatca gcaaattgca caagcattaa
    18481 gaaaatttaa taaaaaagta catcttgttg tcaataaaac agatggaatg aatgaagata
    18541 ttgcttgtgc cgattttcaa tcattaggca ttactgatat acatgctata tctgcatctc
    18601 atggtggagg catcagttca ctactcgaag aaattctcga gccatttaca acagaaacgc
    18661 atgaagcaac tgatgataaa gcgatcaaaa ttgcttttgc ggggcgccct aacgttggaa
    18721 aatcaacttt gataaacagg atattagggg aagaaagagt agttgtttat gatatgcccg
    18781 gaacaactcg agatagtatt tcaataccct ttacaagaga agataagcaa tatgtgctta
    18841 ttgatactgc tggtgtacgg cgtaagtctc gaattgatga aaaaatagaa aagttttctg
    18901 tcatcaaaac tctgcaagct ataaaagaag ctcatgtctg tttgttactt cttgatgcaa
    18961 atgaaggcat taccgatcaa gatatgaatt tgctcggttt tattattgaa tctggtaaag
    19021 cactagttat tgctgtgaat aaatgggatg gcctggaaga agatcataaa gaaaaaataa
    19081 agtcagaact gtcaagaagg ttgcattttg caaattttgc aaaaataaga tttatttcag
    19141 cattgcatgg gagcggggta ggtggattat ttaaagatat taatgaagcc tatcattcag
    19201 caatacaatc cttttctact ccaaaactaa ccagattatt acaagatatc agcgcaaagc
    19261 atacaccacc atgcatcaat ggccgacgaa taaaattacg ctatgctcac cttggcggac
    19321 acaatcctcc agtcattgtt attcatggaa atcaactcga tgccttgcca gaaagttata
    19381 aacgttattt aaacaatgag tttattaagc atttaggatt agtaggtact cctttaaaaa
    19441 tcgagtttaa aggaggtcaa aatccatttg ccaataaaaa aaataaatta tcacaaaggc
    19501 aagtgaacaa gaagaaacga ttaatgcgat gggcaaaaag caagaaatag cactccggtt
    19561 cacctcagcc aggtttaaat tgttctaatg aaaaaaggta ctaatctctt cttattaatc
    19621 tgctttgagt gttaatacat cacaaaccga atgatttact acagcatgcg ctgtacttcc
    19681 tagaatagac ggtaatcctg tagcagagtg tgagcctatt ataattaatt ggcaattcag
    19741 atcttttgct ttgtttaata tatgttcttt tacggacccg atttctacaa attgttgttc
    19801 ctgaggtatt tgtaaacttt ctccaattaa tgataataca gtctgggcat cctcttttga
    19861 tgggttagct agctccgtaa aacccaatcc ttgagctatt aatatacttg ttggcaactc
    19921 aatcacatgt aatagataca attttgcatt aaactgttta gcaatattag cagccttttc
    19981 tgtaaggtgg ctatgctcat ttaacaaatc tgtggcaaat aaaatatttg tatacatgtt
    20041 aacctcattt taaatagaat cattaagtta tagtgtagta gattatgata tctatgtgta
    20101 atttggtatt tttaaattag agtttaattc tcttagagga acaagaacaa aatctcgtaa
    20161 atggaaacaa ggatgtggaa cgattaagtt ttttgtgcga attaatctat aaccatataa
    20221 aatgatatca atatctaatg ttcttggccc ccaaaacttt ctcctgactc gcttgtgcct
    20281 gttttctatt ttctggcacc acttaaggag cttatggggt aacagagtag ttcgtatctc
    20341 tataacgaca ttacaaaatt cctgttgatt ttcaaagccc caggccttat tccaatataa
    20401 gcgagacacc ttgataaggc atgttgatgg aatctgtttg atttcattaa tggcttgtct
    20461 tatttgacgc tcaggatttt tttgattcga tcccaagctc agatagcaaa cattcatttt
    20521 gctattttcc gtttacgacg ttttttaggc cgcccgggcg ttgttgcagc caattgggtt
    20581 accatgttat ttttttcaac attatcgact tcttgaaacg tggcccacca ctgcgccagc
    20641 tccattgact catcaccaga taatgcccta agagccataa aatcatacgc tgctctgaat
    20701 cgaggatgaa gaagtaaatt aaatgctctg ccaccaaatc ttttttcgaa gcgatattgc
    20761 aaaagccaaa tttcccttat aacctggcta taccttttag gtatcgaaat aattgcattc
    20821 tgttcactaa gcactataga catggctttt tcaatcgagg gtaaaggcgg aagcccagat
    20881 tcttgcagtt ttctggaaca ctctatcatt ggaaaccata acaaaacagc aataagaaat
    20941 gccggagtca caggctttcc atcacgtatt cttgtatcag tattttcaag tgctatgccc
    21001 aaaagcggat taaccgggta atgactatca aatagagcag ctgtttgagg aaatagataa
    21061 ttaaataaac catactcgac caataatttt tgaaccgatt cggcttctcc gcattgataa
    21121 agtttcgtta tttcatcaaa tagtcgcgaa ctggaaacat gggttataag gtggcttatt
    21181 ttggggaagg gagctttggt ttccggagat aaattaaatt gcagcttggc actaaacctg
    21241 acagcacgta acatacggac aggatcctct tgataacgcg taacgggatc gccaattatt
    21301 cgaattaact tttgcttaac gtcttctaca ccaccagtaa aatcaatgat tgaagaatca
    21361 gaaatattat aataaagaga gtttattgtg aaatcacgtc tccatgcgtc ttcgtccaga
    21421 gaaccataaa cattatctct tacaagcatg ccgcgttcat taaattggtg gcttgcatcg
    21481 cttgagttat ggccacgaaa agttgctact tcaataattt cacgatgaaa taaaatatgt
    21541 accagtttaa atcgacgacc aatgatacga gcatttttaa aaagattttt tacctcattg
    21601 ggagtcgcat ttgtcgctat atcaaaatct ttaggcgctt tatggagcag taaatctctg
    21661 acactgcctc caacgatata ggcctgaaaa ccatggctga ttaaacggtt aagaacttta
    21721 agcgcattat tacttatatc tgctttcgat atgttatgtt tatttctcgg gatgatataa
    21781 gccaagtcaa ttgcatgtcc atgcgacctg tttcttcgta acagtttttt aataaacttg
    21841 atgattgttg tttctctcta aattgattaa acagtaattc ggttgtgttt tcaattatcc
    21901 tgcaagttta atatttactt tacagaaata ttcaattcta taaagcttaa cacgtaaact
    21961 tttcagatga ctatttaatc taaattgtta acaccaagca attataacga agcaaatgat
    22021 caaagtgaat catttac
//
LOCUS       DACTJE010000039        20920 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.039, whole genome shotgun sequence.
ACCESSION   DACTJE010000039 DACTJE010000000
VERSION     DACTJE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20920
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.039"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            132..383
                     /locus_tag="I9760_001153"
     CDS             132..383
                     /locus_tag="I9760_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042929.1"
                     /translation="MNKLQLTGAALAVGAASMFALAPAFAVDATATTIHCKGVNACKG
                     QGSCKTADNACKGQNSCKGKGILEMSKEDCEKQGGTVAE"
     gene            465..1328
                     /locus_tag="I9760_001154"
     CDS             465..1328
                     /locus_tag="I9760_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HAT5042930.1"
                     /translation="MEVFPNKLAQKLPYLGFGLGLRPNYYEEILTSKPDLDWFEILTE
                     NYLIPGGKPLYYLDKIREHYPIVMHGVSLSLGSSDPLDFDYLKQVQELASRIEPAWIS
                     DHLCWTGVHGLNTHDLLPVPYTSEAIQHIVSRIQVIQDFLKRPFLIENVSSYLTYKQS
                     EMSEWDFILEIVKQSGCYLLLDVNNVYVSSFNHNFDPMAYINSMPPGRVAQIHLAGHT
                     NHGDYIIDTHNAPVIEPVWDLYEATIQRLGPVSTMIERDDNMPDFSELLSEINHAKRL
                     AIQAAKEKVAV"
     gene            1325..2113
                     /locus_tag="I9760_001155"
     CDS             1325..2113
                     /locus_tag="I9760_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HAT5042931.1"
                     /translation="MKSLLTLQTQFQEFIYSGQGSIKESIVPTKLVSTETRLNIYKDG
                     YRLRLIECLAANYPALYAYLGTEEFEKLSARYIDSYPSAYRSIRWYGDRLPELIKKYY
                     AKSCYYLYELAELEWKMTLAFDAADDTVLNVEEMAAVPPESWAEMGFVLHSSVHRLDF
                     CWNVFSLWQALVNNLEIPELKDSPATTGWVLWRGQNYIIQYYSLSEEEAWALDALIQG
                     SSFGVICEGLCQWMSAEEVGMKAASYLKNWIQNGLLSQCRIRRN"
     gene            2165..3538
                     /locus_tag="I9760_001156"
     CDS             2165..3538
                     /locus_tag="I9760_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAT5042932.1"
                     /translation="MLNPREIRTIEDAKRIVEERQLTHVKVGLFDIDGVMLGKYMSRN
                     KFFSALEHGFAFCDVILGWDSKDKLYDNVKYTGWHTGYPDASVRIVPSTCREILFEEN
                     QLLFMAEFSQAAEAICPRGILRRVINKAAGMGYDVYAALEYEFFVFDETPDSVRAKGF
                     RNLKPVTPDNFGYSIIRNTVHAEFYHQILTMAEKMDFPIEGLHTETGPGVLEAAIAVD
                     DAEAAGDKAALFKTFMKILAQRNNKMATFMAKWSGDYPGQSGHIHLSLRDRDGGKSAF
                     YESGREYNMSKIQRHFLAGQQKLMPEFLAMLSPTVNSYSRLIPGYWAPTEATWGVENR
                     TTALRVIPGSDKSQRIEYRLGSADANPYLALAAAIGSGLYGIENELEPEAEVKGNSYE
                     QHHSPALALPRTLWESAQNLKNSSAAYELFGQEFVEHFAASREWEEREFRKHITDWEL
                     DRYFEII"
     gene            3576..4973
                     /locus_tag="I9760_001157"
     CDS             3576..4973
                     /locus_tag="I9760_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAT5042933.1"
                     /translation="MIATLKTYSPVDNSLYVEREYANQEEIKTVLSKAQVAKKLWSLS
                     SLAERQEYCLAAVNEIVASKVDIAEEICWQMGRPIRYAAGEINGFEERARYMIAQAES
                     SLASVELPEKKGFTRYIKREPLGLVLVIAPWNYPYLTAVNAIIPALMAGNVVILKHSA
                     QTPLVAERFDQAFKKAGLPMGVFQYLHLTHEDTEQLMRSPAVNSVAFTGSVAGGERVE
                     QTVAGRFIPVNLELGGKDPAYIRADADLDHAVETVIDGAFFNSGQSCCGIERIYVHKE
                     IYEQFVNKAVNLVKQYKLGRPDDPETTLGPLVRASSADFVREQIKEAVAQGAVAHINT
                     RDFVMDRPGTPYLAPQILTDVNHRMRIMTEETFGPVVGIMSVDSDEEAIERMNDSDYG
                     LTAAVFTQDIHRGIAIGERLQTGTFFINRCDYLDPGLAWTGVKKSGRGCTLSAIGYEY
                     LTRPKSFHIRAKIET"
     gene            5103..6263
                     /locus_tag="I9760_001158"
     CDS             5103..6263
                     /locus_tag="I9760_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAT5042934.1"
                     /translation="MKNQSLIVNWNYPTRILVGAGRINELAKVCRELGMNAPLLVTDP
                     GLASSLMVSKVIQQAQASGLRTGLFCQIKTNPTGDNVMNGVYAFKAGQHDGVIAFGGG
                     SALDAGKAIALMVGQDRPLWDFEDVGDNWTRVNVSAMAPVLAIPTTAGTGSEVGRASV
                     ITDAEKQVKKIIFHPKMLPAMVILDPVLTVGLPKSLTAATGMDALSHCLEAYCSNYYH
                     PMAEGIALEGIRLIKENLIQAYKDGSDLEARTHMLVASAMGATAFQRGLGAMHALAHP
                     LGAIYDVHHGRLNAVLMPYVLLANRSEIENKIEHLANYLSIANGFDGFLDWIEQMRLE
                     LGIENTLSQLGIDHSQIDRLAKMATEDAAAASNPVLFTLEQYKELLSKAIGE"
     gene            6393..7091
                     /locus_tag="I9760_001159"
     CDS             6393..7091
                     /locus_tag="I9760_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HAT5042935.1"
                     /translation="MNLGILLCDKVSEVFVADHGQYPEMFANLLRPADSTLELTVFDA
                     EHGELPTDIHAVDAYLISGSRHGVNDDYPWIRKLEEFVRTLHASQKKLIGICFGHQLI
                     AKALGGKVIKSPKGWGVGMSQNQIYQFKEWMRPSLNCFNLLVSHQDQVIELPTGAEIL
                     AGSDFCPNYMMQIGSFFSVQGHPEFTKSYSRDLMVSREDSVNEIEFAKGMKSLELHED
                     DTIIAQWIVNFLKA"
     gene            7182..9416
                     /gene="lem21"
                     /locus_tag="I9760_001160"
     CDS             7182..9416
                     /gene="lem21"
                     /locus_tag="I9760_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem21"
                     /protein_id="HAT5042936.1"
                     /translation="MAKTIKATGDGACLFNAVSIGLSVEILSGRLDSQLDTPGYQALL
                     DEFAKHHPQFNPKSWKTLKEWLAYYNDTRDIELILAPVLFNLNQKYQDHLDEEILNEL
                     TNLVWKNKANIENGQAWFQLQNTGDLGEALFPKLENLDLKKDRAPLLDKLREILKDYK
                     LELTRENVKQFLTEKAKELLSALKKKISSDPHAFQRGYSCDELKGMTDALAISLVENR
                     EEDITDNRIKIRLENQEEHWNVLCNEEDSERFLDSTPSRLKMTSLEAYRGDKQVSAPT
                     SEQLDLIEEPGVFLRERTIDNPGLGNCAFYAFAIGLVNIIQEEAKYNRRTMFDRWVGL
                     DRSISGQYDEILKLNLEDPDKELLDRLQSSLRIVTYQYQIRELRNVCVFRNGNYNRLT
                     GNSNFVNFAALYYGDPLDTDSRFNPFADSVPILIKMANIDRDSVHPGHENDVLVPLFL
                     DLLYGDTTNPADITLETEPKSDSPIITAMNNITQDFFWGTHLDLNYLAEAFEVNLHVL
                     RNNSPIQEFVDIPERHTLTLTNSNNTHWTTQITTARATTGMLLGRNPHLSGAKRTGEQ
                     QRVNEGFPGKESANDFMIPPFDEISNPHHKKEVETNKPIGKGFFHDRPVVSEEQRKLE
                     QLKRIVANATLAYTTYSESIWFCFFHYHGQTGRDRANNFCKTFSKITDYGQAKEALVH
                     YLNDSRNGNTHPHSYRTMLLHELQGNHDNKKDLQYLSRHFTSSLNKLASVLEVQLFAN
                     RYVP"
     gene            complement(9673..10329)
                     /locus_tag="I9760_001161"
     CDS             complement(9673..10329)
                     /locus_tag="I9760_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAT5042937.1"
                     /translation="MQNIQHLIDYILHIDVYLNAFVSTYGFWTYLALFAVIFCETGLI
                     VTPFLPGDSLLFAAGSIAAQPGNPMNIMLLFVLLFMASILGNQVNFLVGRAIGPRVFS
                     AKRSWLLNPKHLHDTHAFYEKHGGKTIILARFIPIIRTFAPFIAGIGRMRSLHFTLFN
                     LISAILWIGSLLSLGYYLGSIPLIKENFSLVIYGVIAISILPPIIALISKKSTSSIKK
                     "
     gene            10796..11305
                     /locus_tag="I9760_001162"
     CDS             10796..11305
                     /locus_tag="I9760_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042938.1"
                     /translation="MKKWNSFLLLIYLSLFIGHAHAITSLLTNTSLLTNFAQLVKAVE
                     QGDDVKAIIRLDRCQITDPALQTQLVQNLDGASTRFNFTEYLHFRTRINDQLRDTVTT
                     VSSKIFEYSPGVFLSVSSRLNVFEDNTATLRVNFYNPALGTSVLIIDWLCDISNGNDE
                     NGLFLYNSP"
     gene            11627..12919
                     /gene="dctA"
                     /locus_tag="I9760_001163"
     CDS             11627..12919
                     /gene="dctA"
                     /locus_tag="I9760_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HAT5042939.1"
                     /translation="MNFLKKLYVQVLLGVFIGILLGYFLPDVGVQLKAIADVFIKIIK
                     MLISPIIFLTLVSGIAAMNDMRQVGRLAGGALLFFTFITTLALLIGLLATDYFKPGVG
                     LNVNPNSLDAKEASSYLEGAHKVTNVTDLLINIIPRTFISAFVDGELLQVIFISILFA
                     IGLILTGNMGKSLVNGMQILSNVFFKIIHLVMYMAPIAAFAAMAFSVGKFGVGPLFNL
                     IGLLACYYLTSIFFILIILGSLLQWYCKINIWHLLRYLKDELLIVWSTASSETVLPSL
                     MQKLERLGCEQSVVGLILPLGYSFNLAGTAIYLTMAAMFIAQATNTDLTVWQELSLLG
                     VMIISSKGAAGVSGSGFIVLASSLATIGHIPAAGVVLVLGIDKFMNEARSIINMMANA
                     IATIIISRWQQSLDYQKTQFELQEKGQAETKSVYASEM"
     gene            complement(13182..16082)
                     /locus_tag="I9760_001164"
     CDS             complement(13182..16082)
                     /locus_tag="I9760_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042940.1"
                     /translation="MLELNDKETAVIKAIHHNHKKRLESQMITRFGVYFEQSKHPEKL
                     YSWIEKIEDTRLLANHYLDTIALSRAPSTISSLEKETAGSSYYIEKAKLLIPESIRNF
                     LSDINSSNDNTLFNFVAAEVFSLALGKSITDLLFDYFNNEFLGAPENVQKEFNETLEK
                     QTELSNLIKKECERISIEIIKLFHFREMLLLGKISKSPIEPNSREEFIAKAKIANQSG
                     ESVHLAIEIYFARELSRIFNSGFKSLYQVHDVEIQSEHQSPLVKWINQMSESEQVRLA
                     FTQEIQLKFMLLAKDFLLEKMNQKGILARYPIIFSLFAGVIIAPIVGVTMIIMTGGTF
                     LWAIGALAVLVFALSCIATHLLINKLSVLTYQRSSDNRAHIQQSIDMINGEFLRLKKE
                     SLLKKETTSEVIENTRDFQKINQNFHLVTIGKKVARGSVSGWLREYASRYRHSKAIEI
                     DLGDEYKELITQSRIQTQELIHSINKKNSKLLIKWIHDTMTYLQDEKNQSVIEEFELI
                     PKMKEQVLEIVCQLNYIPPMLLKFYSNSREKGGLGGNESDFSHIKRLTPIDAPDKPRH
                     NPYNYLCDTALNLFKKYEPLYTSTCIFLGDPEYRQMLGIARRDAGEPLTAENINRYLD
                     NSYAFLLSLCHKIKPGLGLDPLQQSARVTDEFVLYRMLLLKQLAAISESDSKEISNEI
                     KFKIRLFIQKYFNQDTKIIFDDLENQMFLLNKEAPNSRIYKNSDHFEVIDTELDNIFQ
                     AITLDMAYNSNNFRFIDILDYFINKFIKEYDSQSATVFAYGKAEQEINPQGTKYYLKT
                     LAQYCNNTTEFLKSAQTNTTLTATNLLDCYRYNISLQVCRTQIRIICSIKEINKLKIT
                     NETTEQITLLLKSFQQLAEFAKIHCFLLKTDSSCYRLFKLIENKAMQSTLSRDWIRQL
                     DAKEALVYMMDDLPNQLRLSTDYNVSNLFFSSTKQNKREKMEPLEEHIVKPT"
     gene            complement(16389..17033)
                     /gene="upp"
                     /locus_tag="I9760_001165"
     CDS             complement(16389..17033)
                     /gene="upp"
                     /locus_tag="I9760_001165"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAT5042941.1"
                     /translation="MDFNQVKVINHPLIQHKLTIMRKKETSTVKFRTLMHEVSMLLAY
                     EVTRDLEIEYEEIETPLATMQSPVLKGKKLVFVSILRAGNGLLDGMLQLVPTARIGHI
                     GLYRDPKTLEAVEYYFKLPEHTQDRDVIVVDPMLATGNSAIAAVKEVKALHPKSIKFL
                     CLLASPEGISNFHGEHPDVPIFTAAIDEQLNDHGYIVPGLGDAGDRLYGTKLAH"
     gene            complement(17046..18287)
                     /locus_tag="I9760_001166"
     CDS             complement(17046..18287)
                     /locus_tag="I9760_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="URC4/urg3 family protein"
                     /protein_id="HAT5042942.1"
                     /translation="MNNQEQQIQNVLNTLRDPDTIRTRSHRILNLAKQNRLEHFSLNP
                     ERMTTTASYIVDIITSRYPELDIPYHSRWRHFEMDGLPRIHNLRKQLDPISPSEWGKI
                     LYELVIISVFLDAGAGPLWRYKEALTEKEYSRSEGLALASLYLYESGAFSADPTNPFR
                     VDAERLLDFKETDLIKGFQVSSTNLLEGIPGRVSLLNRLGEIIHRKEHCFNRRKRLGE
                     FYTYVESLQDNKILPTTKLFEAVLETFNDIWPVRLIYHGVSLGDVWIHSALKTNEAGS
                     EYIPFHKLSQWLTYSLVEPLEQTGIDIADLDVLTGLPEYRNGGLLIDTELLKVRNNKI
                     LEQPQDPGSEPIVEWRALTVALLDELALLIRQQLNMNAESLPLAKILQGGTWEAGRQI
                     AQKKRINGTPPIEIISDGTIF"
     gene            complement(18280..19548)
                     /locus_tag="I9760_001167"
     CDS             complement(18280..19548)
                     /locus_tag="I9760_001167"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAT5042943.1"
                     /translation="MSPDDSKKKSKGHIVLSSHPASHTQAPLKIKWGEKNPKERGPVI
                     ASLTNIKNRNAVGTHSGSYSVYRALAVAAGLLDPEHVPDLTDTLPPVDIGPHKQWGEA
                     GKIVSLDPWGHLVASVFSEEIKAGYDIRPTIAVTRAHINIPELHIAMQKGRLKPDGKI
                     LSETGDVVVTKAAIEPVWYLPGIAERFQVSEALLRRTLFEQTAGMFPELVTRSDLDVF
                     LPPIGGLTAYFFGDVTTIHNPEIELTCRIHDECNGSDVFGSDICTCRPYLVHGIELSI
                     ESAQKGGAGLIVYNRKEGRALGEVTKFLVYNARKRQKGGDTAAKYFERTECVAGVQDM
                     RFQELMSDILHWLGITKIHRFVSMSNMKYDALLKSGINVIERVKIPDELIPPDARVEI
                     DAKRAAGYYSPEGIIDINELAKPKGRALHE"
     gene            complement(19634..20842)
                     /locus_tag="I9760_001168"
     CDS             complement(19634..20842)
                     /locus_tag="I9760_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5042944.1"
                     /translation="MSDISRGDIRVKGFTDSEMDFQLIRQLGSSSYRAASVGECLNIA
                     NTMKNEDPEEWVRQFERLAEWQKKDGLERMVKNHVISGREQLFKASNSFRAAEYYSPP
                     SSEHHRKLGLNSADCFCTAISSMDIHFENHSIPYKNINLPCYFISPANDGEKRDTVMI
                     VSGFDGTLEEEFLMRGLAAVERGYNVIHFAGPGQMDVFRKYPDTYFEPDFEQVVKRII
                     NYFEFRQEINMAKLTLMGISFGGYFATRAASHEPRIKVLIANSPILDLHAYMCSFVKM
                     DPCEMPDSEDFSINDLPSIPNEVMPPQLKAQTEHLIRRFGQPTFKATFHYLREFTVGD
                     SLNHIKIPCLALLGSGEGGEPGKQFAEFCKITNSAQYEFSDFEGAGSHCQVGNVSYSN
                     AVVYDWLDSL"
BASE COUNT         6145 a         3822 c         4414 g         6539 t
ORIGIN      
        1 gaaaaaataa ttatttattg gtttggataa tcttgacttt tttgattatt gattcttaaa
       61 atgctattgg caaaacgaga gatctttgcc ggatttataa tcattttcat ttaattaaaa
      121 agggaattgc tatgaataaa ttacaattaa caggcgctgc tttagctgtt ggcgctgctt
      181 ctatgtttgc tttggctcct gcatttgctg ttgatgctac agctaccact attcactgta
      241 aaggtgttaa tgcttgtaaa ggccaaggca gctgtaaaac tgctgataac gcttgcaaag
      301 gtcaaaattc ttgcaaaggc aaaggcattc ttgaaatgtc taaagaagat tgcgaaaaac
      361 aaggtggtac cgttgctgag taattaatag caattatttc agtcgcatct taccaggtat
      421 gtagaacaaa catgcctggt ccccattctt ttttatggtc taatatggaa gtattcccca
      481 ataaattggc tcaaaaatta ccatatttag gttttggttt aggattacgg ccaaactatt
      541 atgaagaaat cctgacgagt aaacctgatt tggattggtt tgaaatatta actgaaaatt
      601 atttaattcc tggaggcaag cctctctatt atctggacaa aattcgcgaa cactatccta
      661 ttgtcatgca cggtgtttct ctttctctgg gaagtagcga tcctttggat ttcgattatt
      721 taaaacaagt acaagaatta gcgtctcgca ttgaaccagc ctggatttca gatcatttat
      781 gttggactgg cgttcatgga ttaaatacgc atgatttatt accggttccg tatacaagtg
      841 aagcgattca gcacatagta tcccgtattc aagtaataca agatttctta aaacgtcctt
      901 ttttgattga aaatgtatca agctacctta catacaaaca gtcagaaatg tcagaatggg
      961 attttattct ggaaatagta aaacaatccg gttgttattt attgttggat gtcaacaatg
     1021 tttatgtcag ttcattcaat cataatttcg atccaatggc ttatattaat tccatgcctc
     1081 ctggaagagt agctcaaatc catttggcag gtcacactaa tcacggagat tacattatag
     1141 acactcacaa tgctccagta attgagccag tttgggatct ttatgaggca accatacaga
     1201 gacttgggcc tgtatctact atgattgaac gtgatgataa catgcctgat tttagcgagc
     1261 ttttaagtga aattaatcat gcaaaacgcc ttgccataca agctgcaaaa gaaaaggtag
     1321 ccgtatgaaa tcgctattga ctttacaaac ccaatttcaa gaattcattt attccgggca
     1381 gggatcaatt aaagagtcca tagttccaac caagttagtt tcaacagaaa cgcgtctgaa
     1441 tatttataaa gacggttatc gattgagatt gattgaatgt ttggcagcga attaccccgc
     1501 tttatatgcg tatttaggta ctgaggaatt tgaaaagtta agtgctcgtt atattgattc
     1561 ttacccatct gcttaccgtt caatccgatg gtatggtgat agattgcctg aattgataaa
     1621 aaagtactat gcgaagtctt gctattattt gtatgaactg gctgagctgg aatggaaaat
     1681 gaccttagcc ttcgatgcag cagatgatac agtgcttaat gtagaagaga tggcagcagt
     1741 gccgcccgaa tcctgggctg agatggggtt tgtacttcat tcatcagtcc ataggcttga
     1801 tttttgttgg aatgtttttt cattgtggca agctttggtc aataatcttg agattccaga
     1861 gttgaaagat agtcctgcta ctactggctg ggtgttatgg cgaggtcaaa attatatcat
     1921 tcaatattac agcttgtcgg aagaagaggc ttgggcttta gacgctctga ttcagggctc
     1981 ctcttttggg gtgatttgtg agggattatg ccagtggatg tctgccgaag aggtcggaat
     2041 gaaagcagcg tcttatttaa aaaactggat tcaaaatggg ttattatctc agtgtcgtat
     2101 tcgtcgcaat tagacattgg gaggtaaaga agcttacatt gacagaacga cataaggata
     2161 tgccatgcta aacccaagag agatccgaac catagaagat gcaaaacgca tcgtggaaga
     2221 aagacaatta actcatgtta aggttggtct ttttgatatc gatggtgtga tgctgggcaa
     2281 gtacatgagc cgtaacaagt ttttttcggc tttggagcat ggttttgctt tttgtgatgt
     2341 cattttagga tgggattcta aagataagct ttatgataat gtgaaatata cgggttggca
     2401 tacgggttac cctgacgcct cagtgcgaat tgttccttca acctgtcgtg aaatactatt
     2461 tgaagaaaat caactgttgt ttatggctga attttcacaa gcagcagaag ccatctgtcc
     2521 tcgcgggatc ttgcgtcgtg taatcaataa agctgcgggt atggggtatg atgtttatgc
     2581 cgcattggaa tatgaatttt ttgtatttga tgaaacacct gacagcgtgc gtgcaaaagg
     2641 atttcgtaat ctaaagcctg ttactcctga taattttggc tactcaatta ttcgtaatac
     2701 ggtacatgct gaattttatc atcaaattct cactatggcg gaaaaaatgg atttccccat
     2761 tgaaggttta catactgaaa caggcccggg agttctggaa gcagcaatag cggttgatga
     2821 tgccgaggcc gcaggagata aagcggcttt gtttaaaaca tttatgaaaa ttctcgcgca
     2881 acgcaataat aaaatggcaa cttttatggc taaatggtca ggtgattacc ctgggcaaag
     2941 tggtcatatt catctgtctt tgcgagatag agatggtggc aagtctgctt tttatgaatc
     3001 aggcagagaa tataatatga gcaaaattca acgccatttt ttagccgggc agcaaaagtt
     3061 gatgcctgaa tttttagcca tgttatcccc gactgtgaat agctattcca gactaattcc
     3121 cggctattgg gctccaacag aggcaacatg gggtgtcgag aaccgcacca ctgctttacg
     3181 agtgattccc ggcagtgata agtcacagcg tattgaatat cgcttgggtt ctgcagatgc
     3241 gaacccatat ctggcgttgg ctgctgccat agggtcgggt ttatatggca ttgagaatga
     3301 gttggagcct gaggctgagg taaaagggaa ttcctacgag caacatcata gtccggcatt
     3361 ggcgttgcct cgcacattat gggaatcggc acaaaattta aaaaactctt ccgctgctta
     3421 cgaattattt ggtcaagaat ttgttgagca ttttgctgct tcaagagaat gggaagagag
     3481 agagtttaga aaacatatta ctgattggga actcgacagg tattttgaaa ttatttaatt
     3541 gaaacgacag attttataaa atttatggat taaaaatgat tgctacctta aaaacctatt
     3601 ctcctgtcga taactctctt tatgttgaga gagagtatgc gaatcaagaa gagataaaaa
     3661 ctgttttaag caaagcacaa gtagcaaaaa aactgtggtc tttgtcttct ctggccgaac
     3721 gccaggaata ttgtttggct gctgtcaatg aaatagtcgc tagcaaagta gacattgcag
     3781 aagaaatttg ttggcaaatg ggaagaccaa ttcgatacgc agctggtgaa atcaatgggt
     3841 ttgaagagag agcaagatat atgattgcac aggccgaatc atcactggct tctgtggagt
     3901 tacctgaaaa aaaaggattt acaagataca tcaaacgaga gccgttagga cttgtgttgg
     3961 ttatagcgcc atggaattac ccctatctca cagcagtcaa tgcgattatt cctgcactta
     4021 tggcgggtaa tgttgtgatt ttgaaacatt ctgcgcaaac tccgttagtg gctgagcgtt
     4081 tcgatcaggc attcaaaaaa gcaggcttac ccatgggtgt ttttcaatat ttgcatttga
     4141 cacatgaaga tacagaacaa ctcatgagat cgccagcagt taattctgtc gctttcacag
     4201 gttcagttgc tggtggtgaa agagtggaac agacagtggc aggacgtttc attcctgtga
     4261 atttggagct aggtggaaaa gatccggctt atatcagagc agacgctgat ttggaccatg
     4321 cagtagaaac agttatagat ggtgcatttt ttaattcagg ccaatcctgt tgtggtatag
     4381 aaaggattta tgttcataaa gagatatacg aacaatttgt taataaagca gttaatttgg
     4441 ttaaacagta taaattggga cggcccgatg atccagaaac cactctgggt ccactggtac
     4501 gtgcttcttc agctgacttt gtcagagagc aaatcaaaga agcggtagcc caaggagcag
     4561 tagcacatat aaatacccgg gattttgtaa tggatagacc tggtacaccc tatctggcac
     4621 cgcaaatttt aaccgatgtt aatcatcgca tgcgaattat gacagaagaa acttttggcc
     4681 cagtcgtagg gatcatgtcg gttgatagtg atgaagaggc aatcgaaagg atgaatgata
     4741 gtgactatgg tttgaccgct gctgtattta ctcaggatat tcatcgagga atagcgattg
     4801 gtgaacgatt acaaacaggt acttttttta tcaatcgttg cgactacctt gatcccggtt
     4861 tggcttggac aggtgtaaaa aaatcaggca ggggttgcac tttatccgcc ataggctatg
     4921 agtatttaac gcggcccaaa tcattccata tcagagctaa aattgagact taaagcctga
     4981 tacctacgat tacctacgcc ttaaaactca aaatataatt ataaaaaaga cctaatcttt
     5041 cctgcggaaa gatacttgct atcagttaca atggtctttt tcgaatcaaa ggaagatcac
     5101 acatgaaaaa ccaatcatta attgtcaatt ggaattaccc gacacgaatc ctggttgggg
     5161 ctgggcgaat caatgaacta gcgaaagtat gtcgtgagtt aggcatgaat gctcctctgt
     5221 tagtcacaga cccgggattg gcttcgtcgt taatggtgag taaagtcata caacaggctc
     5281 aggcatccgg tttgcgaacg gggttatttt gccaaattaa aacaaatcct accggtgaca
     5341 atgtgatgaa tggagtctat gcttttaaag ctggccagca tgatggcgtg attgctttcg
     5401 gaggtggctc ggctttggat gccggcaaag ccatcgcttt aatggttggc caggatcgac
     5461 ctttatggga ttttgaggac gtgggtgata actggactcg ggtgaatgtt tcagccatgg
     5521 ccccagttct tgccattcca acaacagcag gaactggttc agaggtcggg cgagcttctg
     5581 taattaccga tgctgaaaag caggttaaaa aaatcatttt tcatcctaaa atgttgcctg
     5641 ctatggttat tctggatcca gtgttaacag tcggtcttcc caaatcactt actgcagcta
     5701 caggtatgga tgctctttcc cattgtcttg aagcttattg ttccaattat tatcatccta
     5761 tggcagaagg tattgcgttg gaaggaataa gattaatcaa ggagaactta attcaagctt
     5821 acaaggatgg tagtgacctg gaggcaagaa cccatatgct ggttgcttca gcaatggggg
     5881 ctaccgcctt tcagcgtggt cttggagcga tgcatgcctt ggctcatcca ttaggtgcaa
     5941 tttatgatgt ccatcacggg cggcttaatg cggttttgat gccctatgtt ttattggcta
     6001 atcgtagtga aattgaaaac aagatagagc acctggcaaa ttatttgtca atagcaaatg
     6061 gatttgatgg ttttttggat tggatagagc agatgagatt agagttagga atcgaaaata
     6121 cattaagtca gttaggtatt gatcattcgc agatagacag actggcaaaa atggccaccg
     6181 aggatgcggc agcagcttcc aatcctgttc tttttacatt agagcaatac aaagaactat
     6241 tgagcaaagc cattggtgag taatcgttgt aaaggaaaag gtgcagaaac gggttgtttg
     6301 ccaggtataa atcagatgga aaggacttgt atattgaaat taagtctaat ccagacaata
     6361 aaaattttgc gataattaat taggtgaatt aaatgaatct tggtattttg ctttgtgata
     6421 aggtaagtga agtatttgtt gcggatcatg gtcaataccc tgagatgttt gccaatctat
     6481 tgcgcccggc ggattctact cttgaattga ctgtatttga tgcagagcat ggggagctgc
     6541 caacagatat ccatgccgta gacgcttatc tgatatcagg aagtcgacat ggggtgaatg
     6601 atgattatcc ctggataaga aagcttgaag agtttgttcg tactttgcat gcttcccaaa
     6661 aaaaattaat tggtatttgc tttgggcatc aattaatagc gaaagcctta ggtggaaaag
     6721 tgattaaatc tcccaagggt tggggggtag gtatgtcaca aaatcaaatt taccaattca
     6781 aagaatggat gagaccatct ctaaattgct tcaacttgct ggttagccat caagatcagg
     6841 tgattgagtt accaaccggt gcggagatat tggcagggag tgatttttgt ccaaattata
     6901 tgatgcaaat tggttctttc tttagtgtgc aggggcaccc tgagtttact aaatcttatt
     6961 ctcgtgattt gatggtgtct cgcgaggatt cggtcaatga aattgaattt gcaaaaggga
     7021 tgaaatctct tgagttacat gaggatgaca caattattgc ccagtggata gttaattttt
     7081 taaaagcctg atgagctgtt tttaattgat tattataccc ccttagagta caatatgtga
     7141 acattaattt tattcactga ccagaactat cgggagtatg tatggcgaag acaattaagg
     7201 ctactggaga tggcgcttgt ctgtttaatg cagtgtcaat cggactgagt gtcgagatct
     7261 tgagcggaag actggattcg cagctggata cacccggtta tcaggccttg ctggatgaat
     7321 ttgccaaaca tcacccgcaa tttaacccca aaagctggaa aactttaaaa gagtggctag
     7381 cctattacaa cgataccaga gacattgagc tgattttagc acccgtatta tttaatttga
     7441 atcagaaata tcaagatcat ctggatgaag aaattctcaa tgaattaacc aatcttgtat
     7501 ggaaaaataa ggccaatatt gaaaatggcc aagcctggtt tcaattgcaa aacacagggg
     7561 atttgggcga ggcacttttt cccaaattgg aaaatctgga tttgaaaaaa gacagggctc
     7621 ctttattaga taaattacga gaaattctta aagactacaa acttgaactg acacgtgaaa
     7681 atgtaaaaca atttctgacc gaaaaggcta aagaattatt atccgcttta aagaaaaaga
     7741 tcagttcaga tcctcatgcc tttcaaaggg gttatagctg tgatgagtta aagggtatga
     7801 cagatgcctt agcgatcagt ctggttgaga atagagaaga ggacattacg gacaaccgaa
     7861 tcaaaatcag attagagaac caggaagagc attggaatgt attgtgtaat gaagaagata
     7921 gcgagcgttt tctggattcg acaccctcac gtttgaaaat gacttccttg gaggcttatc
     7981 gaggtgataa acaagttagt gcaccaacat ctgagcagtt ggatttgatt gaagagcctg
     8041 gcgttttttt aagagaaaga accattgata accctggcct tggcaattgc gctttttatg
     8101 cctttgcgat aggtttggtg aatataattc aggaagaggc aaagtataat agaagaacca
     8161 tgtttgatcg atgggtaggt ctcgacaggt caatttcagg tcaatacgat gagattttga
     8221 aacttaatct cgaagatccc gataaagaat tactggacag gttgcaaagc tccttaagaa
     8281 tagtgactta tcaatatcaa atacgagaat taagaaatgt ctgtgtattc agaaatggaa
     8341 attataatcg attaactgga aacagtaatt ttgttaattt tgcagcctta tattatggcg
     8401 atcctctgga tacagattct cgattcaatc cttttgccga ttctgttcct atactgatta
     8461 aaatggcaaa cattgatcga gacagtgttc atccaggaca tgaaaacgat gtattggttc
     8521 ctctcttttt agatttatta tatggtgaca ctacgaatcc agctgatatc accctggaaa
     8581 cagaaccaaa gagtgattca cctatcatta ctgcgatgaa taacatcact caggattttt
     8641 tctggggcac ccatttggat ttaaattatt tagccgaagc gtttgaggta aatcttcatg
     8701 tgctaaggaa taatagtcca attcaagagt ttgtcgatat tcccgagcga cacactctta
     8761 ctttaactaa tagcaataat acccattgga ctacccaaat tacaacggcg agagccacta
     8821 caggaatgct tttggggcga aaccctcacc tgagtggagc taaaagaacc ggggagcaac
     8881 agcgagttaa cgaggggttt cctggaaaag aatcagccaa cgattttatg atacctcctt
     8941 ttgatgaaat atctaacccc catcataaga aagaggtaga gaccaataaa ccaatcggta
     9001 aagggttttt ccatgataga ccggtagtta gtgaagagca gagaaaattg gagcaattaa
     9061 aacgaattgt tgccaatgct acgctcgctt acactaccta cagtgaaagc atctggtttt
     9121 gcttctttca ttatcatggt caaacaggcc gagatcgggc taataacttc tgcaaaacat
     9181 tttctaaaat aacagattat ggccaagcca aagaagcttt ggttcattat ttaaatgaca
     9241 gcaggaatgg aaatacacat ccacattcct acagaacaat gttactgcat gaattacaag
     9301 ggaatcatga caataaaaaa gatttgcaat atttgtccag gcattttact tcttctttaa
     9361 ataaactagc atctgtttta gaagtgcaat tgtttgccaa cagatatgtt ccttaggtaa
     9421 ctgttcatgg ggtatgtgat taaggaatat taggtcctat gtctttcccg cgaaggcggg
     9481 aatctatcca taaagctagc acaagggttg atttttagtt agcttcccgc cttcgcggga
     9541 aagacaactc aaataaatag gcttataaac agaaaaattt gcataccccg tgtaaaggtt
     9601 acttccttag taagagcgtg gtgtaaaagg catcctagag ccctttgcac ccgcataagt
     9661 ccctaaagta tattattttt taattgagga cgtcgatttt ttactaatta aagcgatgat
     9721 gggcggtaaa attgaaatcg ctatgacccc atagataacc aatgagaaat tctcttttat
     9781 taatgggatt gagcctaaat aatatcccag gctaagaaga cttccaatcc aaagtatggc
     9841 gctgatcaga ttgaaaagcg tgaaatgcaa tgatcgcatc ctgccaatac ctgcaataaa
     9901 tggagcaaag gtgcgtatta tgggaataaa acgcgccagt attattgttt tgccgccatg
     9961 cttctcataa aaagcatggg tatcatgcag atgcttggga ttcagtaacc aggagcgttt
    10021 agcgctaaag acacgagggc ctattgcacg ccctacaagg aaattcactt gattccctaa
    10081 tatcgaagcc atgaaaagca aaacaaacaa cagcatgata ttcatagggt ttcctggttg
    10141 agctgctata cttcctgcag caaataacag ggagtctcct ggtaagaaag gagtgacgat
    10201 aaggcctgtc tcacaaaaga tgacagcaaa taacgcaaga taagtccaaa acccatatgt
    10261 tgatacaaag gcatttaaat aaacatcgat atgaagaatg tagtcaatca ggtgctgtat
    10321 gttttgcata gtgtctcttt ggattaaatg tatgacgtga attccagggt atctatactg
    10381 acgtgagtta tggataaaat cagctgttat ctcaaaccac tcgggcatct cctcagcctg
    10441 aacggctccc tgggtatcga ctggttctta tataatcttt attcatcact cacgttactc
    10501 taaagaaaca aaaggatacc cattttgaaa atatagagtt tatgaaaaat tttgatcttt
    10561 ttaactcgac ctcgaaattt atacccgtga aagcttactt tttcatcagc ccaaaatatt
    10621 attgttttta ttacctttta aaaagcttac taaattctac acgacttaca taaaatcccc
    10681 aattttctta aaagaaaatg aaattttctg gcaaatatgg attattgatt atacacttta
    10741 tgtaatattt gttcttatag attaagcaaa taatgaatca ataagagggg acgttatgaa
    10801 gaaatggaat agctttcttt tgttaattta tttgtcttta tttataggcc atgcccatgc
    10861 aattacttct ttgttaacta acacttcctt gttgactaat tttgcgcaat tagttaaagc
    10921 agttgaacaa ggtgatgatg tcaaggccat tatacgattg gatcgatgcc aaattacaga
    10981 tcctgcactg caaactcaat tagtacaaaa tttggatggc gcttcaacaa gattcaattt
    11041 tacagagtat ttgcattttc gaaccagaat taatgatcaa ttacgagata cggtaactac
    11101 tgtctcaagc aagatatttg aatattcgcc gggtgtattt ttatctgttt caagtcgttt
    11161 gaatgtcttt gaagataata ctgccacctt gcgtgtgaat ttctataatc ccgccttagg
    11221 tacaagcgta ttaattatcg attggctttg tgatatcagt aatggcaatg atgaaaatgg
    11281 gttgtttcta tacaatagtc cttaagctct cgaggtataa agtttacttc tttcatttgg
    11341 ctcatttctt aatgactgtt cagatccagt ctaagatgat ctggacttat ccaataataa
    11401 tgatgcatca agcaaacctg aagcaatcaa ttctgcaatt ttatttttca aaatctcttc
    11461 aaattgttgt aataatagtt aaaattgaaa aaatatatca atattgatca tatctagaat
    11521 aatttaaact attcatgtaa taatgatttg tctagggaaa gaatttttag tgcttcatgc
    11581 tttgcttttg aatagttgtt tatagtaaac gacctggaga ataaaaatga attttcttaa
    11641 aaaattatat gtacaagttc ttctgggtgt tttcattggt attttgcttg gatatttttt
    11701 gcctgatgta ggggttcaat taaaagctat agccgatgtt tttatcaaaa tcattaagat
    11761 gctaatttct cctattatat ttctcaccct ggtctccgga atcgcagcaa tgaatgatat
    11821 gaggcaagtt gggcgcttgg caggtggggc attgcttttt tttacgttca tcacaactct
    11881 ggcattgcta ataggtcttc tggcgacaga ttattttaag cctggtgttg gtttaaatgt
    11941 gaatcccaat tctctggatg ccaaggaagc gagttcatac cttgaggggg cacataaggt
    12001 aaccaatgtg actgatctgc taattaatat tatcccgaga acttttatta gcgcttttgt
    12061 cgatggtgag ctgctgcaag ttatttttat ttcgatattg tttgctatag gtctgattct
    12121 tacaggcaat atgggcaaat ctttggttaa tgggatgcaa atcctgtcta atgtcttttt
    12181 taaaatcatt catcttgtaa tgtatatggc gcctatagct gcctttgctg ctatggcgtt
    12241 cagcgttggg aaatttggag ttggcccttt gtttaattta attggattat tggcttgtta
    12301 ttatctgacc tccattttct ttatcttgat tatcttggga agtcttttgc aatggtactg
    12361 caaaattaat atctggcatc tgttacgata tttaaaggat gagcttttaa tcgtatggag
    12421 tactgcttcc tccgaaaccg tactgccaag tttgatgcag aaactggagc gattggggtg
    12481 tgagcaatct gtagtcggtt tgatcttgcc attaggatat tcttttaatt tggcaggaac
    12541 tgcaatctat ttaacaatgg cagctatgtt tattgcacag gctaccaata ctgatttaac
    12601 tgtttggcag gaactgtcgc ttctgggagt tatgatcatt agttccaagg gtgccgctgg
    12661 ggtgtccgga agcggattta ttgtgttggc aagctcatta gctacgatag gacatattcc
    12721 tgctgcaggt gtcgttcttg ttttgggtat cgataaattc atgaatgaag ccaggtcaat
    12781 tattaatatg atggctaatg caatcgccac gattattatt tccaggtggc aacaatcttt
    12841 ggattaccag aaaactcaat ttgaattgca agaaaaagga caagcagaaa ccaaaagtgt
    12901 ttatgctagt gagatgtaaa atttctacag gactcacgaa aaaccccaat ctcatcgtga
    12961 aaaatgtttt taattcggtc atttagttta tctaagctcc ctcattaaaa acattttttg
    13021 cctcgacctt ggagtttttg gtaagtcccg ttctattgcg aaagggggca tgtaacccct
    13081 caaaaaaatc aagcttatag aaaaacctgg atgtccttgt attagccatc caggctccaa
    13141 aataccgagt ttttatttac caataatacc tctaaattaa tttaggttgg tttcactata
    13201 tgttcttcca agggttccat tttttctctt ttattttgct ttgtcgaact aaaaaataaa
    13261 tttgaaacgt tgtaatctgt agaaagtctt aactgattag gcagatcatc catcatgtaa
    13321 acgagcgctt cttttgcatc gagctgtctt atccaatctc tggataaagt agattgcatt
    13381 gctttgtttt caatcagttt aaataatcga taacaagaag agtcagtctt taacaagaaa
    13441 caatggattt ttgcgaactc tgcaagttgt tgaaaggatt tgagtagaag ggttatttgt
    13501 tcagtagtct catttgttat ttttaattta ttgatttcct ttatgctaca gataatccga
    13561 atttgcgttc tacaaacttg caaactgatg ttgtatcgat agcaatcaag gagatttgtg
    13621 gccgttaatg ttgtattggt ttgagcgctt tttaaaaatt cggtcgtatt gttacagtat
    13681 tgagcaagag ttttgagata gtattttgtg ccttgagggt tgatttcctg ttctgctttt
    13741 ccataagcaa atactgttgc ggactgggag tcgtattctt taataaattt attaatgaag
    13801 tagtcaagaa tatcaatgaa tctgaaatta ttggagttat aagccatatc aagagtgatg
    13861 gcttgaaaaa tgttatccaa ttccgtatca attacctcaa aatgatcgct gtttttatag
    13921 atacgggagt tgggggcttc cttgtttagc aaaaacattt ggttttcaag atcatcaaaa
    13981 atgattttgg tatcctgatt aaagtatttt tgaataaaaa gcctaatctt aaatttgatt
    14041 tcattgctta tttctttgct atcagattcg gaaattgctg ccagctgttt taacaacagc
    14101 attctgtaga gaacgaattc atcagtgaca cgcgctgatt gttgtaaggg atccaagcct
    14161 aatccaggtt tgattttatg gcaaagcgat aataaaaagg cataggagtt atcaagataa
    14221 cgattgatgt tttccgctgt tagaggctcc ccggcatctc ttctggcgat acctaacatt
    14281 tgtcgatatt ccgggtctcc taaaaaaata caagtcgaag tatataaagg ctcatatttt
    14341 ttaaacagat tcagagctgt atcgcagaga taattatagg ggttatgcct gggtttatct
    14401 ggcgcatcaa tgggggtcaa acgttttata tgagaaaaat cagattcatt acctcccagg
    14461 ccacctttct ctctggagtt agaatagaat ttcaataaca taggggggat gtaattgagt
    14521 tgacagacaa tctcaaggac ttgctctttc attttgggga ttaattcaaa ttcttcaatc
    14581 acagattgat ttttttcatc ctgcaaataa gtcatggtat cgtgaatcca cttaatcaat
    14641 aacttggagt ttttcttgtt aatcgaatga atgagttctt gagtttgtat cctgctttga
    14701 gtaattaatt ctttgtattc atcccccaaa tcaatttcga tggctttact gtgcctatac
    14761 cgagaagcgt attctcttaa ccatccagat actgagcctc tggctacctt tttaccaatc
    14821 gtgactaaat gaaaattctg gttgattttt tgaaaatctc ttgtattttc aataacctca
    14881 ctggttgttt ctttttttaa taaactttct tttttcaaac gtaaaaactc tccatttatc
    14941 atgtcgatag attgctgaat atgagctcta ttatccgaag aacgttggta agttaatacg
    15001 ctgagtttat tgatcaggag gtgcgttgct atgcaagaga gagcaaaaac caggacagct
    15061 agagcaccta tcgcccatag aaaagtaccg ccagtcataa taatcatagt cacaccgact
    15121 attggagcaa taatgactcc tgcaaagagt gaaaaaatga tagggtatct tgcgagtatc
    15181 cctttctgat tcattttttc cagtaaaaaa tcttttgcca gaagcatgaa tttcaactgg
    15241 atttcctgag taaaggccaa tcgaacctgt tcagactctg acatttgatt gatccacttg
    15301 accagaggcg attgatgttc actttgaatt tccacatcgt ggacttgata aagtgattta
    15361 aaacctgagt taaaaatacg acttaattca cgtgcaaaat agatttctat agccaaatga
    15421 acagattcgc cagattggtt ggcaattttt gctttggcaa tgaattcctc tctggaattg
    15481 ggttcgattg gtgatttgct tattttccca agcagcaaca tttctctgaa atgaaataat
    15541 ttgataattt ctatggagat gcgctcacat tcttttttta tcaaattgga taattcagtt
    15601 tgtttttcca gggtttcatt aaattctttt tgtacattct ctggcgcccc taaaaattca
    15661 ttattgaagt aatcgaataa caggtcggta attgatttgc ctaaagcgag agaaaagacc
    15721 tcggcggcta caaaattgaa taaggtattg tcattggaag aattgatatc agaaagaaaa
    15781 tttcttatag attccgggat aagcagtttt gccttttcaa tgtagtagga agaacctgct
    15841 gtttcctttt ccaatgaaga tattgtgctt ggagctcggc ttagagcgat agtgtcgaga
    15901 tagtgattag ctaagaggcg cgtgtcttca attttttcta tccaggaata taatttttca
    15961 ggatgttttg attgctcaaa atatacccca aaacgggtta tcatttggct ttcaagtcgc
    16021 ttcttatggt tatgatgaat agccttaatg actgcagttt ctttgtcgtt cagttctaac
    16081 attcttcgct ccctcgtaga gtgattagat atgactttat gcaataaggg ctatgccaag
    16141 agactatatt tgctgttgct tgtttcctgg catttccctg ccaaaattct ttagaatctt
    16201 gatacatcac atctgttgtt cccgcataaa cactcagagg gagtccacag aaaaattggt
    16261 ggtaattttt cagcatgccc ttgtcaaaaa gtgaaagcag tcattactga aagggcaaga
    16321 caatcttaat tattatttaa acgtactatt ggctgattag gtatccctga aataaggata
    16381 attcagcatt aatgagccag ttttgtgcca tatagcctgt ctcctgcgtc tcctaaacca
    16441 ggaacaatgt atccatgatc attcagctgc tcatctatgg ctgcggtgaa tattgggaca
    16501 tcaggatgtt ctccatgaaa attggaaatt ccttcaggag aggccagtag acaaagaaat
    16561 ttaattgact tgggatgtaa tgccttgacc tctttaaccg cggcaatggc tgaatttcct
    16621 gtcgccagca tggggtcaac aacaataaca tctctgtctt gtgtgtgttc aggtagcttg
    16681 aagtagtatt cgactgcttc cagcgttttg ggatctcgat agagacctat atgaccaatt
    16741 cgggcagtag gaactaattg taacatgccg tccagtagac cattacccgc gcgtaatatc
    16801 gagacaaaaa caagtttttt tcctttcaaa acaggtgatt gcatagtggc caaaggagtt
    16861 tctatttctt cgtactctat ttccaaatcc cgagtgactt cataggctaa tagcatactg
    16921 acttcgtgca tgagtgtccg aaattttact gtgctggttt ctttttttct cattatggtg
    16981 agtttgtgct gaatgagagg atgatttata accttaactt gattgaagtc cattattatt
    17041 ccttattaaa atatcgtgcc atcactgata atttctattg ggggtgttcc attaattcgt
    17101 ttcttttgtg caatctgtcg tcccgcctcc caagtgcctc cttgcagtat tttggctaag
    17161 ggaagagatt ctgcattcat gttgagctgt tgcctgatta atagcgctag ctcatccagc
    17221 aaggctacgg ttaaagcacg ccattctact atgggctctg agccagggtc ttgaggttgc
    17281 tccagaattt tattatttct tacttttaat agttcggtat cgattagtaa accaccattt
    17341 cgatactcag gcaatccggt caatacatcc aaatcagcaa tatcaatacc tgtttgttcc
    17401 agtggttcta ctaaagaata agtgagccat tgtgataatt tgtgaaaggg tatgtattca
    17461 gatccggcct cattggtttt caatgcgctg tggatccaga catcgcctaa agatactcca
    17521 tgatagatca atcgaacagg ccatatgtca ttgaatgttt ctaacacggc ctcgaataat
    17581 ttggtagtag gcagtatttt gttgtcttgc aaggattcca cataagtata aaattcacca
    17641 agacgttttc ggcgattaaa acagtgttcc ttgcgatgaa tgatttctcc aaggcgatta
    17701 agcaaggata ctcgaccggg tattccttca agcagattgg tagagctaac ctgaaaacct
    17761 tttatgagat ccgtttcttt aaagtcaagc agacgctcgg catccacgcg aaacgggtta
    17821 gttgggtcag cgctaaatgc cccactttca tagaggtata aactggccag ggctaatcct
    17881 tcagagcgac tgtactcctt ttcagtgaga gcttctttat agcgccatag ggggcctgcc
    17941 cctgcatcga ggaaaacact gatgatgacc agttcgtaga gtatttttcc ccattctgag
    18001 ggggaaattg gatccagttg tttgcgtaaa ttatgaattc ggggaaggcc atccatttca
    18061 aaatgacgcc agcggctgtg atatggaata tcaagctcag gataacggct ggtgataatg
    18121 tctacgatat acgatgccgt tgttgtcatt cgttccgggt tcagggaaaa gtgttccagc
    18181 cgattttgtt ttgccaagtt taaaatacga tgcgaccggg ttcttatggt gtcaggatca
    18241 cgaagcgtat tcaaaacatt ttgtatttgc tgctcttgat tattcatgaa gggcacgtcc
    18301 tttcggttta gccaattcgt tgatatcaat aattccttcg ggtgagtaat atcctgctgc
    18361 acgcttggca tctatttcta cccgggcatc tggaggaatc aactcatccg gtatttttac
    18421 ccgttcaata acattgattc cagatttgag caaggcatcg tatttcatat tggacattga
    18481 aacgaagcga tgaattttag tgatccccag ccaatgaaga atatcggaca ttaattcctg
    18541 gaaacgcata tcctgaacac cggcaacgca ttccgttctt tcaaagtact tggctgcagt
    18601 gtcaccacct ttctgtcttt ttctggcgtt ataaaccaga aatttagtga cctccccaag
    18661 agctcttcct tctttgcgat tataaacgat aaggcctgcg cctccttttt gtgcggattc
    18721 gatggataat tcgattccat ggaccaaata tggtctgcaa gtgcatatgt cagaaccaaa
    18781 gacatcggag ccattacatt catcatggat acggcaagtt aattcgattt ccggattatg
    18841 gatggtagtc acatcaccaa agaaataagc agttaatcct ccaatgggcg gcaaaaagac
    18901 atccaagtcg ctacgggtca ccagttcagg gaacattcca gcggtttgtt caaaaagagt
    18961 gcgtctgagc aatgcttctg aaacctggaa gcgctccgca attccaggca agtaccagac
    19021 tggttcaatc gcggccttag tcaccacgac atcccctgtt tcacttaaaa tttttccatc
    19081 aggttttaat ctgccttttt gcatggcaat atgcaattcc gggatattaa tatgtgccct
    19141 tgtgacggca atggttggtc tgatgtcata gccggctttt atttcttctg agaaaacaga
    19201 agccactaga tgcccccagg gatcgaggga gacaattttg cctgcttcac cccattgctt
    19261 atgaggccct atgtcaacag gaggcagggt atcggtcaga tcaggaacgt gttcgggatc
    19321 cagcagacca gctgctactg ccaaggccct gtaaacagag taagaccctg agtgagttcc
    19381 aactgcattt cgatttttga tattagtgag agaagcaata acaggccccc tctctttagg
    19441 gtttttttca ccccatttga tttttaatgg agcttgtgtg tggcttgccg ggtgtgaaga
    19501 caagacaatg tgtccttttg attttttctt tgaatcatct ggtgacatga tacttaattc
    19561 cttttgtttc aacggtgctc aaataaatcg gttgttttat cttttatctt ttataagcag
    19621 gtgcacactg ttgttataaa ctgtctaacc aatcataaac cacagcattg gaataagaaa
    19681 cattgcctac ctgacaatga cttccagccc cttcaaaatc actaaattca tattgtgcag
    19741 aattcgttat tttgcaaaat tccgcgaatt gttttccagg ctctcctcct tcgccacttc
    19801 ctaataatgc aaggcaaggg attttaatat gatttaatga atcacctacg gtgaactctc
    19861 ttaaataatg aaatgttgcc ttaaaagtag gttgcccaaa tcttctgatt aaatgttctg
    19921 tttgggcttt gagttgaggg ggcatcactt cattgggtat agagggtaag tcattgatgc
    19981 taaaatcttc agaatcaggc atttcacaag ggtccatttt tacaaaggaa cacatataag
    20041 catgtaaatc cagaataggg gagttagcaa ttaaaacttt gattcttggt tcatggctcg
    20101 cggcacgagt tgcgaaatag ccgccaaaac taatgcccat caatgtcaat ttggccatat
    20161 tgatctcttg cctgaactca aaataattaa tgattctttt aaccacttgc tcaaaatcag
    20221 gctcaaaata agtgtccgga tattttcgaa agacatccat ttggccgggg cctgcgaaat
    20281 gaatcacatt atacccacgt tctaccgcag ctaatccgcg catgaggaac tcttcttcca
    20341 aagttccatc aaagccactg actatcatga cggtatctcg tttttctcca tcattggcag
    20401 gggagataaa atagcatggg agattgatgt ttttataagg aatggaatgg ttttcaaaat
    20461 ggatatccat gcttgagatg gctgtgcaaa agcaatctgc tgaattcaat cccaattttc
    20521 tgtgatgttc tgaggaagga ggggagtaat attcagcggc acgaaatgaa ttggaggctt
    20581 tgaacaattg ctctcgaccg cttatgacat ggttttttac catgcgttcc aaaccgtcct
    20641 ttttttgcca ctcggctaac cgttcaaact gtctgaccca ttcttcaggg tcttcatttt
    20701 tcatggtgtt tgcaatattt aaacattcac ctactgatgc ggctctatag ctgctggatc
    20761 ccagttgccg gattagttga aaatccatct ctgaatcagt aaaacccttc acccgaatat
    20821 cgccacgact gatatcactc attttacttc tccgttattt ccgttttaca gagtgttatg
    20881 agcttaatag taacggtaca tgccaagaac atcaatctta
//
LOCUS       DACTJE010000040        20526 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.040, whole genome shotgun sequence.
ACCESSION   DACTJE010000040 DACTJE010000000
VERSION     DACTJE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20526
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.040"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..129
                     /locus_tag="I9760_001169"
     CDS             <1..129
                     /locus_tag="I9760_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HAT5042945.1"
                     /translation="IGFFKEHPGHYTEEVAKKIPGDMKDEFTDIEPSSTKPSSTGS"
     gene            500..1537
                     /locus_tag="I9760_001170"
     CDS             500..1537
                     /locus_tag="I9760_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAT5042946.1"
                     /translation="MLFRHKGILYLFLSSLIISAANPVIAKLITLGNEHLVNGRNPIS
                     FCNVLFAGNFIAVLTLILIHFRELKEFQPEHLTAKNWCLIFLAALFAGFLTPTLFFLG
                     LMYTNVINVVLISNLQIPMTLLAGWIFLGEVPNIRVTTGAFLATLGVFAIVILQYWLG
                     GAGALTPNPEMKTGPLFIFLSSIPRSGEICIFLAVVSSTASTIIGFHALRRLPHYLFS
                     ILKMLLGLVLFFFIVISMFGWHHFADLFSPFLWEWMLFYGSVIIALRYYFSMLGMKHA
                     KVAEVAISSSIIPLMSIIFSYLILGEVPGISQIIGGSLILFGIYVALTGKLQAPEKTE
                     ILEKPSGFSGV"
     gene            complement(1639..1884)
                     /locus_tag="I9760_001171"
     CDS             complement(1639..1884)
                     /locus_tag="I9760_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042947.1"
                     /translation="MNVHSMCFSRVLRYAAVTSLLFMAVSFTSVANAAQGCGEGYHRA
                     IHNGTCVLNYPGAFATPAPAHPGCWRNMWGQLRCYRY"
     gene            2214..2864
                     /locus_tag="I9760_001172"
     CDS             2214..2864
                     /locus_tag="I9760_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HAT5042948.1"
                     /translation="MDRFQDRYQAGQLLGDKLKPYANRNDVIVLALPRGGVPVAAMIS
                     EAINAPLDLMIVRKLGVPGHEELAFGALAINKIKVYNEELLQYLQLSQKDINQVLSQE
                     ENELAKRNRAYRGDKPFPDLKNKIVILVDDGIATGASMRAAVLALRKESPKKIIVAVP
                     VAPEDMNDIMKSVADEYICLISPMSFNAVGLWYEDFSQTEDEEVLYLLGKVYDRKV"
     gene            complement(3020..3751)
                     /locus_tag="I9760_001173"
     CDS             complement(3020..3751)
                     /locus_tag="I9760_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HAT5042949.1"
                     /translation="MKKFILFPLISALSSTFVHAGGMGDSNSCCSTFASLEGGYTWSS
                     IDGYNFTIVGTNNTLTSTEDKQGYSGRLAVGVLSMIDDQYGFTGEVGWGYYGRTTINP
                     SVAGALGQVPAALTIKHTLSGFDALLGVTYFQNYFSWSAKAGALIQNMQVDSSAFFTP
                     QIFPIVDNFDMKTNRTAVLPAAKLGVAYNFDSNWAITGSYLFALGANPGTTATFNPNT
                     LRSSLSIDDENPMMNAILFGVQYTA"
     gene            3922..5208
                     /locus_tag="I9760_001174"
     CDS             3922..5208
                     /locus_tag="I9760_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5042950.1"
                     /translation="MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHS
                     PVTSLLATMASSGVALVVRPLGGFILAVWADKYGHHKALMFVFYLMAAATMLITFTPS
                     YSTIGLVATCVIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
                     LMGSSACLILTFYCSNQTLYQFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDT
                     GKLFILVKQQKRSLLIAFSLVAGCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGI
                     LVSIITIPFFGALSDRIGKKIVMLCALACYFGLIYPLLFLLNQYPGVTILILVENILG
                     LCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNSAVLLFGGFAPFIITALIEYTKNPMV
                     FTYYQMTTVFISLLAVLAYQEERKLSENQPEHLVAI"
     gene            5220..6374
                     /gene="argE"
                     /locus_tag="I9760_001175"
     CDS             5220..6374
                     /gene="argE"
                     /locus_tag="I9760_001175"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAT5042951.1"
                     /translation="MTTLDWLKQLISFDTTSRNSNLSLIEYLANGLNDFKINPILIHD
                     SKEQKANLLATLPGKQGRLEGGIILSGHTDVVPVDGQIWDSDPFQATVKNNKVYGRGA
                     CDMKGFIAVVMALVPQLKEMNLDFPVHFAFSYDEEIGCLGVPSLIDKIVELNYQPRAC
                     IVGEPTLMKPVVGHKGKYSYRCQIHGVAAHSSLTNQGCNAIEHAASFISYLRGMANEF
                     KKYGNRDFSYDVPYTTLTTNLIKGGNAYNTIPNLCEFVFEFRNLSTDSSEDLNQKIMS
                     YVKEELAPSLHQDHPDSNVNLDTIAKAPGLDMPITDPFVRAAQTVCQSDKLMKVAYAT
                     EAGLFQQAEIPTIVCGPGSIEQAHRANEYVEVEQLRLCEEFIINMLRSHF"
     gene            6728..7414
                     /locus_tag="I9760_001176"
     CDS             6728..7414
                     /locus_tag="I9760_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAT5042952.1"
                     /translation="MSKFIFVIGGTGTGKTTVSEEIKTIAEAQNFTVTILSLDHYYLP
                     QSMMDPDKPKNFDVPEALEQTLIQQHLQDLEAGKTIFRPTYDMESSDRVLNGEMKIIP
                     ADIIIVEGIFAGEYLSSLRNQTEKLKIYLQSNQINDNYTRKEDRDIVERKRSSAYAHV
                     AKKNQMAGFFRYVAPHMISSNMVIDNPWLPQQNMTTTEKLPLIAGNNLIKLNEFLSSA
                     ESHFLYKTYS"
     gene            complement(7479..9491)
                     /gene="ravR"
                     /locus_tag="I9760_001177"
     CDS             complement(7479..9491)
                     /gene="ravR"
                     /locus_tag="I9760_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavR"
                     /protein_id="HAT5042953.1"
                     /translation="MLSQGVRSMGKEKRELEASYIPLPKKGRFWIHSESCYTEEELNF
                     REWILKIRSNSGVHPDYLNNPSSWFANDDQALVNLYEHAWFYSNAYKPRHVLSLDDFH
                     VQPIDNQRLQWPQLYDLMQKVGDYLLLRNKELPPEHLNQPMNFVLLDVNNILKGLSQN
                     NNIQQVKEQLESVTRYLRTIEKNISPLIGSDRLFLANFRKTVDTKIHPHLDHMLETQL
                     LRDKLGELSKDIRKLSSERNRILHYALNANSVNPHSYEFELDKISDSASYPTQAAKHC
                     AQKSNELVVDPLPILHLTKEQLSDCPHFKLITMKDEILDHYAKAVSDLNELERFQKVI
                     QEIMDLLGQAGEVYTVHQFKHQMQSLLTQIDSFIDASSEHIDEIIDANTQAYHKAIQE
                     EQNLPFWKNWLTNEKEKLKIFIKNQDTLSQFPSSSSDLKKTNKALKTRVNEVVSHLNQ
                     PKLKETSFEAIAGQTQALNKLMLSMHDWMKIQHELKGLPLPQPPEPLKIMPTPIPTVD
                     VFSSPPPPLHAYKPLFTALPGNTTAPCMPDNNQCKTTPSSPTQNSLGYLGLIALIPIS
                     VIALYLVIHWMNKKDAQIRVSGTEEEFNKIKEKFEDLLAELKGFEHSEEIDLSDDYYF
                     IVQDYEELIKKTKSNHYDVEALQDTYQELEDLYEEYCNPKKELLNF"
     gene            9751..10236
                     /locus_tag="I9760_001178"
     CDS             9751..10236
                     /locus_tag="I9760_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAT5042954.1"
                     /translation="MPSFDIVSKMNEVDLRNAVDNAVREVSTRFDFRGVEATIELKDL
                     TVTLRSESDFQVRQLEDLFRNHCSKRNLSTSGVEIEDEPVHSGKFYTLTMTFKQGIDQ
                     PTAKEIVKYIKDTKAKVQTSIQGDKVRVTGKKRDDLQETIALLKKSNIELPLQYENFR
                     D"
     gene            complement(10359..12626)
                     /locus_tag="I9760_001179"
     CDS             complement(10359..12626)
                     /locus_tag="I9760_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5042955.1"
                     /translation="MDNSFVFNETTLKRVPHFLVLIIILLLGLPYIGLNWGLDFSNLA
                     ITINDGKIAGSQLIESQIRGYFRQTLLQWSGFSLAAVTVLLAFTQYRLSNDKIALIIG
                     LAILFSGSIEALHTLVIDGLALKTADKNNLDALIWVFSNSVSGFILIIGLSLLAHASD
                     DKPVRLVTFILLTTFLVLAAIAVIYYAAVIIQIPDMWLYDSYVSRPFELISVFIYLML
                     MIIVYPKIYASYPTILSDSVFYMSLTQVVLAIYIMLLSTTPYDSAFNIAYFLKVVVYF
                     IPCTCFVINYVFSYSAVLEGQKNLKIKQEELKYMASHDPLTNLFNRREFEDLLDKSIA
                     NGQRNNSSLALLLIDLDNFKAINDTFGHFHGDELLKQFANRLVLLVRKGDLLSRVGGD
                     EFTLISLNIESPSSARHLAERILNELNSPYTINGKLITVTVSIGISIFPDDGNTTEDL
                     LRKADLAMYKAKNSGKNTYQFYTEQLGYSQHRESELESHLRKALQNDEFELYYQPKFN
                     LINMEIIGAEILLRWNNEALGGVSPNEFIPVAENTGLITELSQWVLQKSCIQIMHWAS
                     QYNKMLSFSINVSPVQLTNPQFPKILEKILQEYQYPAEYLELEITENALMGDQDEISK
                     MLNVISDLGIKLSLDDFGKEYSSLNRLKTLPIDSLKIDKTFISDIHNEHEKVVIIDII
                     IKLANELGMSIVAEGIETIPQLKYLVSRKCHFGQGFLLSKPLTANEFTSIAYTKLGKT
                     KSNAKVISLINNNRL"
     gene            complement(12780..14123)
                     /gene="amrB"
                     /locus_tag="I9760_001180"
     CDS             complement(12780..14123)
                     /gene="amrB"
                     /locus_tag="I9760_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HAT5042956.1"
                     /translation="MSVRQPAVAGYFYPDDPLTLKQTVLNFLDQAPIHKPAPKAILVP
                     HAGYVYSGAVAASAYASLRDKKDTINKIILLGPAHRLYFKGIAYDPVDKFATPLGEID
                     QDKELLTQIIDLPYVYSLPEAHQNEHCLEVQLPFCQMIFSKFKILPLVIGETNPQDVA
                     RLIARIWGGDDTLLIISSDLSHYLPYHIAQREDKKTCLSIDTLNGEEILHDAACGYFP
                     LRGFLYFGRQNHVYSRLLDLRNSGDTAGDKERVVGYAAYHFYQKLKFSEHCADELKYI
                     AKETIRLQTEENKALTINYNDYSQLLQMRVPTFITLKKNGMLRGCMGSLTTKDPLAEN
                     IIYNSIRAASADPRFPQIKPCELKELSLTISLIKPLSPLHFDSEEELKSQLQIGLDGL
                     VLICGPYQATFLPSVWESVKTKDEFINHLKLKMGLSENFWSSEMRALRYATEIIK"
     gene            14195..15271
                     /gene="amrS"
                     /locus_tag="I9760_001181"
     CDS             14195..15271
                     /gene="amrS"
                     /locus_tag="I9760_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HAT5042957.1"
                     /translation="MFDTIPTKYWHKLEDGRIQCDVCPRNCKLREGQRGLCFVRANLD
                     NQIKLTTYGRSSGYCIDPIEKKPLNHFLPGSPVLSFGTAGCNLACKFCQNWDISKSRD
                     IDTLADTAMPEDIAKAAQNLNCLSVAYTYNDPVIFMEYAIDVAKECHKLGIHSVAVSA
                     GYINPEPRRELYTYMDSANIDLKSFSEDFYYKITGSHLQPVLDTLIYLKKETSVWFEI
                     TNLVIPEENDSDAEFERMSEWIMTNLGPDVPLHFTAFHPDWKMMDKAPTPFSTLQRAR
                     EIAIKTGLHYVYTGNVHDTTGSSTYCPHCKLPVIVRDWYRILDYRVDDTGHCLNCHTQ
                     IAGVFKGAVGHWGPRRVMVKLGCS"
     gene            15330..15749
                     /locus_tag="I9760_001182"
     CDS             15330..15749
                     /locus_tag="I9760_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1171 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042958.1"
                     /translation="MRFQDSDFEERYNTMWNKIAVSADVQIRQLFGAKGFFSEQQPNY
                     YQLLANYAQAAKNIVDNLNRQSPMFDDKEYVEGYMIATLQSVYKDFSQYKPRIAGRYG
                     EHSSCVELINKTLDWVQSFDLKLENLSESDDEMKITF"
     gene            15913..17385
                     /locus_tag="I9760_001183"
     CDS             15913..17385
                     /locus_tag="I9760_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAT5042959.1"
                     /translation="MKQLVVAGFFLLLLTSPLSALDVDTYRSSQKQFNTEDKAEQGDA
                     QAQFELGLKYEKGDGVNKDLKKAIYWYQKAADQGQAEAQNNLGVLYLKGEGVPQNSQQ
                     AMYWFKKASEQGLAIGQNNIGILYENGLGVKKDPGQAFIWYQKAAEGGNSDGQYNLAV
                     MYMYGNGIPKDIKKAIHWYIKAAEQGDLDAQNNLGVLYERGEEVPRDLKAAISWYTRA
                     ANEGSLIAQTNLGVLYMTGDPSIQDGKKAIYWYEKAAAQGGEKAQNNLGYIYEQGIGT
                     EKDMKKAIYWYEKAAENGFTLAQNNLGVLYSNDGELQDYKKAYLWFKKAADQGFAEAQ
                     NNLGLMYMKGNGLSVNYHEAVLWYKRAAEQGLPLAQHNLAIMYMKGLGIKKDNKLAIK
                     WYQKAAEKGLDLAQNNLAVMYIRGEGVKRDFKKAMYWYQKAAEQGLDLAQINLGIMYL
                     DGMGVNKDFAKAKYWIGKAKDSGSQDALTIWNKNKLWKYQ"
     gene            17481..19067
                     /locus_tag="I9760_001184"
     CDS             17481..19067
                     /locus_tag="I9760_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAT5042960.1"
                     /translation="MSAARQINNPKMRQWRILLVYNIYRIVSIFLFLGIYYFSYINKY
                     YPLLFLCIAFAYLVFALIFLYFWHRKILSFDKQVFLSGTIDVIAISSMLGLIGNLESG
                     YGILLNVTIAALSILVPGRLAIYFASLASCLLLCGNVLQFFIYNQKDLSTFFYSGIYG
                     AGFFATAITAWYLSNWVRMSESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRI
                     RLMNTAAREFFNLSKHHAIQKLDQLSDSLVSKFDDFLLKTTNNERTAQTIIEDPYLRV
                     HFFSTSVGHKSEVLIILEDMTYIAQQAQQLKLAALGRFSASIAHELRNPLGAIAHAIQ
                     LFGDNEDLNEENTRLKQLIMNNCERMNRVIKNVLQLSRRQKSQPQVNELATFLEHFKQ
                     DFTHHNPCNLTIKLPANKSLSVVFDKSQLEQILVILCENSIQHGRDHEGNVNIVIAAK
                     SSSNKITLTVCDSGPGVPPEHRDNIFEPFFTTLRGGTGMGLFIARDLCEINQARLNLL
                     NSNKGSCFAITVNPSDELLI"
     gene            19064..20392
                     /locus_tag="I9760_001185"
     CDS             19064..20392
                     /locus_tag="I9760_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAT5042961.1"
                     /translation="MIKSKVLVIDDEPDIRELLTLTLSRMGLICDAVSDFKQGIEHIK
                     QNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQLPVAVITAYGNVEGAVNTLKAGAFDYV
                     SKPVDLKMLRELVKTALSMQNPSTESPDEGLLVGESLIMQQLRESIHKLARSQAPVFI
                     QGDSGVGKELVAQLIHANGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDK
                     TGLFVAAHGGTLFLDEIAELPLAMQVKLLRAIQEKAIKPIGELYEIPVDVRILSASHK
                     NLIEEINAGRFRQDLYYRINVIELRVPTLMERLSDIPCLTEAILKKLSKNQNRNLVKI
                     NPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPAVAVPSTS
                     TNLNGYLQDQEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD"
BASE COUNT         6288 a         3434 c         4145 g         6659 t
ORIGIN      
        1 attggatttt ttaaagaaca tcctggtcat tatacagaag aagttgctaa aaaaatacca
       61 ggagatatga aggatgagtt tactgatatt gaaccatctt ccactaaacc atcgtcaacc
      121 ggcagctaat tttttattga gcatagacga gcaacggata aatagaaact gtggtttttt
      181 atcttgtaaa taaacctgga tttaattgta ttctttcctt ttatcttctc ttattgacgt
      241 gtattgtgct gtaagggaga tttataaagt aaatttttag ctgcacttaa gaaatctaaa
      301 gaaattttat gtattgattc gcaagataag attattaaaa tcgtgcatat tctatccatg
      361 ttatttgatt gtgcatcagg ggggggctaa tcaagatatg ctattgattg cagtctctca
      421 cctgaaaaaa gatgtagata caggtaaaat tgagtgtaca aaattaaagc cttgataagg
      481 atgtgggggg gaaattaaga tgctgtttcg acataaggga atactatatt tattcctgag
      541 ttcgctcata ataagtgctg ctaatcctgt catagcaaaa cttatcactc ttgggaatga
      601 gcatttggtc aatggtcgta acccaatttc gttttgtaat gtcctatttg ccggaaactt
      661 cattgcagtg ctcactttga ttttaattca ttttagagag ctaaaagaat ttcaaccaga
      721 acatttaaca gcgaaaaatt ggtgtttaat ttttctggct gctttattcg caggtttttt
      781 aacgcccacc ttattttttt tgggattaat gtacaccaat gtgattaatg ttgttcttat
      841 ttccaatcta cagatcccta tgactttatt agccggctgg attttcttgg gagaggtgcc
      901 taatattcga gtaaccacag gggctttttt agctaccttg ggagtatttg caatagttat
      961 tcttcaatat tggctggggg gagcaggtgc tttaactcca aaccctgaaa tgaaaacagg
     1021 tcctcttttt atttttctca gctctattcc tcgttcaggt gaaatttgta tctttttggc
     1081 agtagtttcc agtactgctt caactattat tggttttcat gcacttagaa gattacccca
     1141 ttatctgttt agtattttaa aaatgttatt ggggcttgtt ttattctttt ttatagtgat
     1201 ttctatgttt ggctggcatc attttgctga tcttttttcc ccatttttat gggagtggat
     1261 gctcttttat gggagtgtta ttattgcttt gcgatattat ttcagtatgc tgggcatgaa
     1321 acatgcaaaa gttgcagagg ttgcaatcag ttcatcaata attcctctga tgtctattat
     1381 tttttcctat ttgatattag gtgaagttcc cggtatttcg caaatcatag gtggaagttt
     1441 aattttgttc gggatatacg ttgctttaac aggaaaatta caggctcccg agaaaaccga
     1501 aatcttggaa aaaccttcgg gtttctcagg tgtttagaat gactattttt ctatgaataa
     1561 aaccttattt ttgctagtta tatcaaaaat ataacagcaa gaaataaggt ttgatatgtt
     1621 tgtattttca agtttgaatt agtatctata gcaacgcaat tgtccccaca tattacgcca
     1681 gcagcctgga tgtgctggcg caggggttgc aaaggcgcct ggatagttta gaacacaagt
     1741 accattatga atggccctgt gataaccttc cccgcatccc tgagccgcat ttgccactga
     1801 tgtgaaggac acagccataa acagaaggct tgtcacagca gcataacgta acaccctcga
     1861 aaaacacatg ctatgtacat tcattgtact ctccttatgt tgcttcaatt attttttagc
     1921 atacagcata agtatagagt caatttttaa attatgccta aaatgtgatc atattttttg
     1981 gttagtcata tttcgaatct atagattaat ccattctata tattccttga tgcgccattt
     2041 tgaggtacag ggttattctc tgtatctgaa gtcaactcta atgacgaaat agtgtacggg
     2101 tagttattca gctaaaaatc agagaagatt aagaataaac tcttgctgac agcaggataa
     2161 tttccagatc tgaactacaa taaaaatagc aggacagata attgagtagg aaaatggatc
     2221 gctttcaaga tcgttatcaa gctggacaat tattaggaga taagcttaag ccttacgcga
     2281 accgaaatga tgtcatagtg cttgcactac cccgtggagg agtgcctgta gctgcaatga
     2341 tttctgaggc tataaatgct ccattagatt taatgattgt tcgcaaactg ggtgttcctg
     2401 ggcatgaaga attagctttc ggtgctttgg ccattaataa aataaaagtt tataatgaag
     2461 agctgttgca atatttgcaa ctttctcaaa aagacattaa tcaggttctt tctcaagaag
     2521 aaaacgaatt agcaaagcgg aatagagctt atcgtggtga caaacctttt cctgatttaa
     2581 aaaataaaat cgtaattctg gtagatgacg gtattgccac aggcgctagt atgcgtgcag
     2641 ctgttcttgc tttacggaag gagtctccta aaaaaattat agtagcggtt cctgtagcac
     2701 ctgaagatat gaatgacata atgaaaagtg ttgcggatga atacatttgt cttatatctc
     2761 ccatgtcttt taacgctgtg ggtctttggt atgaagactt ttcccagact gaggatgaag
     2821 aagtattata tttacttgga aaagtatacg atagaaaagt ctaaaaataa caaatgacaa
     2881 accatgacaa aatgaaagag aggagctttt atctcctttt tttgagtcct gtgaagacct
     2941 gttgaaggaa taagttaaaa agtcttgggg ccaaatactt gaccccatat aaaaggtgac
     3001 gtttttaaat aatattaaac taagcggtat attgaacccc aaataaaata gcattcatca
     3061 tgggattttc atcgtcaata ctcagactgg atcttaatgt gttaggattg aaagtcgcgg
     3121 ttgttcctgg atttgctcca agcgcgaaca aataagagcc ggtaatagcc cagttagaat
     3181 caaagttata ggcaactccc agctttgctg ctggcaaaac agcagtcctg ttcgttttca
     3241 tatcgaaatt atccactata ggaaatattt ggggagtgaa aaaggcacta gagtctactt
     3301 gcatattttg aatgagcgcg cccgccttgg cagaccagct aaaatagttt tgaaaatagg
     3361 taacgccgag cagggcatca aatccgctca aggtatgctt gatcgtcaag gcagctggaa
     3421 cctggcctaa agctcctgca acacttggat taatggttgt tctaccatag tacccccagc
     3481 ctacttcgcc ggtgaatcca tattgatcat ctatcatact aagcacaccg acagccagac
     3541 ggccactata accttgttta tcttctgtcg aggtaagagt attgttggta ccgactatag
     3601 taaaattgta tccatcaatg gaactccagg tgtatccacc ctcaagtgac gcaaaagtag
     3661 agcaacaaga gttggaatca cccataccac cagcatggac aaaagtactg gacagggcgg
     3721 aaattaaagg gaacaggata aattttttca tgatttagta actcattcca tgtttattat
     3781 tgtaaaagtc aataatttaa atcagttgat agggaattac cagtaaaatt aatattgatc
     3841 ttagtttctt ataaagcatg gtaatttttt ttatattcta ctagtatgtg ctttgctcta
     3901 aatttaccct cgtaaggaaa catgaataca tcaaatacct cctttaaaac attgggagca
     3961 atatgtattg gtaatgctat ggaatggtat gatttcatga tttataactt catgctgctt
     4021 tttattgccc aagctttttt tcccaatcat agtcctgtga cttccctctt ggctacaatg
     4081 gcaagctctg gagtggctct ggtagtccgg cctcttggtg gttttatttt agcagtttgg
     4141 gcagataagt atgggcatca taaagcatta atgttcgttt tttatttgat ggctgccgca
     4201 accatgctta ttacttttac accatcctac tccacaatag gtctggttgc tacttgtgtc
     4261 attgtatttg cgagattgtt gcaaggactt gctactggtg gcgagtttgg catatccagc
     4321 gctctattag ttagcttatc gccaagcgat aaaaaggggt tttacacttc cttacaaatg
     4381 gtaggccagc ttttggcaat attaatggga tcttcagcgt gccttatttt aactttctat
     4441 tgttccaatc aaacccttta tcaatttgcc tggagaatac cttttgcttt aggactgttg
     4501 attctacctt taggatggat tttaagacgt cgattgcaac gggaatttac ctggaagaaa
     4561 gagcaacagg acacaggaaa gttgtttatc ttggtaaagc aacaaaaacg ttctctgttg
     4621 atagctttta gtcttgtagc cgggtgtaca ggctcgattt ataccttatt cagctatatg
     4681 cctacctatt gtaaattgta cttgaattta tcgctgacag aggcgtactt aggcactgca
     4741 tctggaatat tagtcagtat tattactatt cccttttttg gagcattgtc ggatagaata
     4801 ggcaaaaaaa tagttatgtt atgtgctttg gcttgttatt ttggattaat ttatccttta
     4861 cttttcttac tcaaccaata ccctggtgta actattctca ttttggttga aaacatttta
     4921 ggtttgtgta tcggcgctta ttttggagtg ttgaccatta cggttagctc cttatttcca
     4981 ccatcagtac gttctacttg tctagccgtc agctataatt cagctgttct gttgtttggt
     5041 ggttttgctc catttattat aacggcgttg attgaataca ctaaaaaccc tatggttttt
     5101 acttattatc aaatgacgac ggtctttatc agtttgctag cagtccttgc ttatcaggag
     5161 gaaaggaagc tatctgagaa tcagccagaa catctcgttg caatttaaaa ggtaggaata
     5221 tgacaacttt agattggtta aaacaactca tttcttttga tactacttcg cggaactcta
     5281 atttatcatt gattgaatat ttggcgaatg ggttaaatga tttcaagatt aatcctatcc
     5341 taattcacga tagtaaagag cagaaagcta atctgttagc tactttgcca ggaaaacaag
     5401 ggcgtttgga agggggtatt attctgtctg gtcatactga tgttgttcct gttgacggtc
     5461 aaatttggga tagtgatcct tttcaagcca ctgttaaaaa taacaaagtg tatggtcgtg
     5521 gcgcgtgtga catgaaagga tttattgcag tcgtgatggc tttagtccca caattgaaag
     5581 aaatgaacct ggattttcca gttcattttg cattttctta tgatgaggaa attggttgtc
     5641 ttggtgtgcc ttctctcatc gataaaatag tggagcttaa ttatcaaccg cgtgcttgca
     5701 ttgtaggtga gccaacctta atgaagcctg ttgtgggtca taaaggcaaa tattcttatc
     5761 gctgccaaat acatggtgta gctgcccatt cttcattaac gaatcaagga tgtaatgcga
     5821 tcgaacatgc cgcgtctttt ataagctatc ttcgagggat ggctaatgaa tttaaaaaat
     5881 atggaaatcg agatttttct tatgatgttc catatacaac acttaccact aatctcatca
     5941 aaggaggtaa tgcttataat actattccaa acttgtgtga gtttgttttt gaatttagaa
     6001 atctgtctac tgacagctca gaggatttaa atcagaaaat tatgtcttat gttaaggaag
     6061 agcttgctcc tagcctgcat caggaccatc ctgactctaa tgttaacctt gatacgatag
     6121 caaaagctcc tggtttggat atgccgatta cagatccttt tgtcagagca gctcaaacag
     6181 tttgccagag cgataagctg atgaaagtag cttacgctac agaagccggg ctatttcaac
     6241 aggcagaaat tcctactatt gtgtgtggtc caggtagtat cgaacaagcc catagagcaa
     6301 atgaatatgt tgaagtagag caattacgat tatgtgagga atttataatc aatatgctgc
     6361 gctctcattt ttgagcttta tcagattggt aagtaaaaaa tcatcggtat ttgatgctga
     6421 ttttactagc acttttataa ggtattcaca aagatataca cagtttttgt ggataacatg
     6481 attagatgaa atttgattgg ttttaaaaaa atactttttt atcaattggt tattaaaata
     6541 aatgtttttt atgccagcat ttgctgtaat cattttattt tagctacaat ctttaaaatg
     6601 gttcgctttg taagctttgg agtactggat ttggctcaag agcttatcaa ggtacagatt
     6661 gatcggtttt cttgtaattt gttcctcagt tattataatg gcttttttga ccgcaagaga
     6721 atattacgtg tctaaattta tctttgtcat tggaggaact ggaactggaa aaacgactgt
     6781 ttcagaagaa attaagacaa tagcagaagc acaaaatttc acggttacga tattaagtct
     6841 ggatcattac tacttgcccc aaagtatgat ggatccggac aaaccaaaga attttgatgt
     6901 ccctgaagct ttggaacaaa cattaattca acagcatttg caggatttag aagctggtaa
     6961 aaccatattc agaccgacct atgatatgga aagcagtgac agagtattga atggggaaat
     7021 gaaaattatt cctgctgata tcattattgt cgaaggaatt tttgctggtg agtatttatc
     7081 ctctttaagg aatcaaacag aaaaactcaa aatttattta caatccaatc aaataaatga
     7141 caattacaca aggaaggaag acagagatat agttgaaaga aaaagatcat cggcttatgc
     7201 ccatgttgcg aaaaagaacc aaatggcagg attttttaga tatgtcgctc cccatatgat
     7261 ttcttcaaat atggtcattg ataatccttg gcttcctcaa caaaacatga ctactaccga
     7321 aaaacttcct ttgattgcag gcaataactt aataaaatta aatgaatttt tatcttcagc
     7381 tgagagtcat tttttatata aaacatatag ttaaaatgta acttattgcc ccaggggata
     7441 atttaggggc aatataaaat gcaatattta aaattgagtt aaaagttcag taattccttt
     7501 ttaggattac aatactcttc atagagatct tccaattctt gataagtatc ttgtaatgct
     7561 tctacatcat aatggtttga ttttgtcttt tttatcaatt cctcatagtc ttggacaatg
     7621 aaataatagt catctgagag atcaatctct tccgaatgct cgaaaccttt aagttcggct
     7681 aatagatctt caaatttttc ttttatctta ttgaactctt cttctgtgcc agatactcgt
     7741 atttgagcat cttttttatt catccaatga ataaccaaat ataaggctat gacactaata
     7801 ggtatcaggg caattaatcc taaataacct aaagaatttt gtgtgggtga gctgggggtt
     7861 gttttgcatt gattattgtc aggcatacaa ggagctgttg tattgccagg cagggcagta
     7921 aatagaggct tatatgcatg aagtggaggc gggggagaag agaatacatc aactgttggt
     7981 ataggagttg gcattatctt gaggggttca ggtggttggg gaagtggaag ccctttcaat
     8041 tcatgttgga ttttcatcca gtcgtgcatt gataacatca atttattaag tgcttgagtt
     8101 tgtccagcta ttgcttcaaa ggacgtttct tttaacttag gctgatttaa atggctaacg
     8161 acttcattga cccgggtttt taaagccttg tttgtttttt tgagatcaga agaactggat
     8221 ggaaattgag agagtgtgtc ctgattttta ataaaaatct ttagtttctc tttttcatta
     8281 gtcaaccaat ttttccagaa gggtaaattt tgttcctctt gtattgcctt atgataggct
     8341 tgagtgttcg catcaattat ttcatcaatg tgttctgacg aagcgtcaat aaaactatca
     8401 atttgtgtca gcagagattg catttgatgt ttgaattgat gaacagtata gacttcgcca
     8461 gcctgcccca gaagatccat gatttcctgg atgaccttct gaaatcgttc caactcgtta
     8521 agatcagaga ctgctttggc atagtgatca aggatctcat ctttcatcgt gattaatttg
     8581 aagtgaggac aatcacttaa ttgctcttta gttaaatgca gtataggcag aggatctacg
     8641 accaattcat ttgatttctg agcgcagtgt ttggctgctt gagttggata agaagcggag
     8701 tcactgattt tatccagttc aaattcataa gagtggggat ttaccgaatt ggcattaaga
     8761 gcataatgca agattctatt tcgttcactg gataattttc tgatgtcttt tgataactct
     8821 cccaacttat ctctaagtag ttgagtttcc agcatatggt ccagatgcgg gtgaatttta
     8881 gtatccactg ttttgcgaaa gttggctaaa aataatcggt ccgaaccaat cagtggtgaa
     8941 atattttttt caatagtacg caggtagcga gtgactgact caagttgctc ttttacttgt
     9001 tgtatgttgt tgttctgact tagccctttt aagatattgt ttacatcaag aaggacaaaa
     9061 ttcatgggtt ggtttaagtg ttcggggggt aactccttat ttcttaataa aagatagtca
     9121 ccaacttttt gcattaagtc ataaagttgt ggccattgga ggcgctgatt atcaatgggt
     9181 tggacatgaa aatcatcaag gcttaacaca tgtctcggct tgtaggcatt tgaataaaac
     9241 caggcatgtt cataaagatt aaccaaagct tgatcgtcgt tggcaaacca ggaactggga
     9301 ttattaagat aatccggatg aacccctgaa ttcgatcgaa tttttaaaat ccattctctg
     9361 aagtttaatt cttcctccgt gtagcaactt tcagaatgga tccaaaagcg ccctttctta
     9421 ggtaacggta tatatgaggc ttccagctct ctcttttcct ttcccatact tctcactcct
     9481 tgagaaagca aaaaaaaact taaggtaatt ccagattaac actaaattag caatagcttt
     9541 ttttgctgcc gattaataat atccataacc aactgggtaa tttttattcc aatttatttg
     9601 ataacactct ttgcggaatt aagcaatagt taatcaattg atttgttttg agacaaatat
     9661 aatttttaca agagggaaaa tggaggtgct atttctttca cctatccctg ttttttgata
     9721 aattacaatt ttaattttaa cggattgttt atgccttctt ttgatattgt ttctaaaatg
     9781 aatgaagtgg atttgcgcaa tgcagtagat aatgcagtgc gagaagtaag cactcgattt
     9841 gattttcgag gagttgaggc tactatcgag cttaaagatc tgactgttac tttgcgttct
     9901 gaatctgatt ttcaagtgag gcaacttgag gatttattcc gaaatcattg ctcaaaacgt
     9961 aacctcagca catcaggagt agagatagaa gatgaacctg tacatagtgg aaaattctat
    10021 actcttacca tgacttttaa acaaggcatt gaccaaccta ctgctaaaga gatagtgaag
    10081 tacataaaag acactaaggc taaggtccaa acttctattc agggtgataa agtgagggtg
    10141 accggaaaaa agcgagatga tcttcaagaa accattgctt tattaaaaaa atcaaatatt
    10201 gaattacctt tgcagtatga aaattttcgt gattaattaa tatgaattcg atataaaagg
    10261 caaagaaaag ccttttatat ccttaaatga acgatatcac ctaaaatgca cagcatctat
    10321 aagaaataca ttttgaaaga tccagtatat ggttctaatt aaagtctgtt attgttaatt
    10381 aatgaaatga cttttgcgtt agatttggtt tttcccaact tggtataagc aattgaagtg
    10441 aactcatttg cagttaaggg cttactcagg aggaagcctt gaccaaaatg acattttctt
    10501 gacacaagat attttaattg aggtatggtt tcaataccct cggcaacaat actcattcct
    10561 aactcattag ccaatttaat aataatgtct ataatgacta ctttctcatg ttcgttgtgt
    10621 atatcagaga taaatgtttt atcaattttt aaactgtcaa tagggagagt ttttagtctg
    10681 ttaagggagg agtattcctt gccaaaatca tccaatgaca actttattcc caagtcggaa
    10741 attacattaa gcattttgga gatttcatct tgatcaccca ttaaagcatt ttcagtaatt
    10801 tcaagctcga gatattctgc agggtattga tattcctgta gtattttttc aagaatttta
    10861 ggaaattgtg gatttgttaa ttgaacaggc gaaacattta tggaaaagga aagcatcttg
    10921 ttgtattgac tagcccagtg cattatttgt atgcacgatt tttgtaaaac ccattgactc
    10981 agttctgtta ttaatccggt gttttctgct actggtataa actcattagg agacactcct
    11041 cccagggctt cattattcca gcgtaaaaga atttctgcgc caataatctc catattaatc
    11101 aggttaaatt tgggttggta ataaagttca aattcatcat tttgtaatgc ttttcgaaga
    11161 tgagattcca gctcggattc cctatgctgt gaataaccta gttgttcagt ataaaattga
    11221 taggtatttt ttcctgagtt ttttgcttta tacatagcca agtccgcttt tcttaacaga
    11281 tcttctgtag tatttccatc atcaggaaaa atagatattc ctatactgac cgttactgtg
    11341 attaatttgc cattgatagt atagggactg tttaattcat ttaaaattcg ttctgccaga
    11401 tgccttgcgc tggaagggga ttctatattc agagagatta atgtaaattc atctccaccc
    11461 actctggata aaagatcacc ttttcgaact agtaaaacca ggcgattggc aaattgtttt
    11521 aaaagttcat cgccatgaaa atgaccaaat gtgtcattta ttgccttgaa attatctaaa
    11581 tcaataagca gtaaagccag ggaagagtta tttctttggc cattagcaat ggatttatca
    11641 agcaaatctt caaattccct gcgattaaac aggtttgtca gaggatcatg agaggccata
    11701 tattttaact cttcctgttt tatttttaag tttttttgcc cttccagtac cgctgagtat
    11761 gaaaaaacgt aattaattac aaaacaggtg caaggtataa aataaacaac cactttaaga
    11821 aaatatgcta tattaaaggc actgtcatac ggagttgttg ataatagcat gatgtagata
    11881 gccaacacga cttgggtgag tgacatatag aatacagaat cagataaaat agtcgggtaa
    11941 gatgcataga tttttgggta aacaataatc atcaacataa gatagataaa gacggatatg
    12001 agttcaaaag gtctggaaac ataagaatca taaagccaca tatcaggaat ttgtattatt
    12061 acagcggcat aataaatgac tgcgatagca gccaagacca aaaaagtagt cagtagaata
    12121 aaagttacca atctcactgg tttatcatcg cttgcatggg ccagtaaaga aagtccgatg
    12181 attagaataa agccgctaac actattagag aaaacccaaa ttaaggcatc caggttattc
    12241 ttatctgctg ttttcaaggc taagccatca ataaccaagg tatgaagtgc ttcaatggag
    12301 ccagagaata gaatggcaag gccaataatt aaagcaattt tatcattact cagtcgatac
    12361 tgagtgaacg ctaataacac ggtgactgca gcaagagaaa atcctgacca ttgtaagaga
    12421 gtttgtctga agtaaccgcg aatttgagac tcaatgagtt gggaacctgc tattttccca
    12481 tcatttattg taatggccaa attactaaag tccaaacccc agtttaaacc tatgtaaggc
    12541 aggcctaaca ataaaataat aattaatacc aaaaaatggg gtacacgctt taaggtcgtt
    12601 tcattgaata caaatgaatt atccattttt gtatctcaaa attcctatat tttaaaatta
    12661 gcataaaatg tgcctgttat tggcaaaatt gctgatttta tatcttgtta atctgggcta
    12721 tatgaaaata atatttgggg ctggattctt atttgagcat tattaatgag tttttcaatt
    12781 tattttatta tttctgttgc gtagcgaagt gctctcattt ctgaagacca gaagttttct
    12841 gaaagcccca tttttaattt taaatgatta ataaactcat cttttgtttt aacagactcc
    12901 catacagaag gtaaaaaagt agcttgatag ggaccacaaa ttaatactaa accatcgaga
    12961 ccaatttgca gttgtgattt gagctcttct tcactgtcaa aatgtaaagg agataatggt
    13021 ttaattaacg aaatagttag tgacagctct tttagttcac atggctttat ttgggggaag
    13081 cgtgggtctg cacttgctgc ccgtatggaa ttgtaaatga tgttctcagc taatggatct
    13141 ttagttgtca ggcttcccat acaacctctt agcatgccat tcttttttag agtaataaaa
    13201 gtaggaactc gcatctgtaa taattgacta taatcattgt aattaatggt taatgcttta
    13261 ttttcttctg tttgtaatcg tattgtttct tttgcgatgt acttcaattc atctgcgcaa
    13321 tgctctgaaa atttaagttt ttggtagaag tgataagcgg catagcctac tactctttcc
    13381 ttatctccag ccgtatcgcc tgagttgcgt aaatcgagca gacggctgta aacatgattt
    13441 tggcgaccga aatacaggaa accgcgcaga ggaaaataac cacaagcggc atcgtgaagt
    13501 atttcttcac catttaaagt gtctatggaa aggcaagtct ttttatcttc tctttgggct
    13561 atgtgatagg gaagataatg gcttaaatca gagctgataa tcaggagggt atcatcacca
    13621 ccccatattc gggctattag acgggctacg tcttgaggat ttgtttctcc aataaccagg
    13681 gggagtattt taaatttgga gaatatcatt tggcagaaag gcaattgtac ttccagacaa
    13741 tgttcatttt ggtgagcttc aggaagtgaa tagacatagg gtagatctat aatttgagtt
    13801 agcagctctt tatcttgatc aatttctcct aacggcgtgg caaatttatc aacaggatca
    13861 taggcaatgc ctttgaaata caatctatgc gcagggccta atagtatgat tttattgatg
    13921 gtgtcttttt tatcgcgtaa cgaggcataa gcagaagctg ctacagctcc tgaatagaca
    13981 taaccagcat gtggaaccag tattgctttt ggagctggct tatgaattgg tgcttggtcc
    14041 aggaaattca atacggtttg ctttaaagtc aatggatcgt caggataaaa atacccagct
    14101 acggctggtt gacggacgct cacaacaaca ctccggaaca actgaacata ctatacttat
    14161 aaatatagac taagagttga ttgtgggcta tttgatgttt gatactattc caaccaaata
    14221 ctggcataag ctggaagatg ggcgcataca atgtgatgta tgtcctcgta attgtaaatt
    14281 gcgtgaaggg caaagagggt tatgttttgt tcgtgcaaac ctggataatc aaataaaatt
    14341 aactacctat ggccgttcta gcggttattg catagatccc attgaaaaaa aaccattgaa
    14401 tcactttttg ccgggtagcc ctgtcttatc tttcggaact gcgggatgta atctagcctg
    14461 taagttttgc caaaattggg acattagcaa atccagagat attgatacat tggcagatac
    14521 tgccatgcca gaagatattg caaaagctgc ccaaaacctg aattgcctaa gtgtggcata
    14581 tacctataat gatccggtga tttttatgga atatgccatt gacgtcgcaa aggaatgtca
    14641 taagcttggc attcattcgg tagcggtttc tgcagggtat attaatcctg agccacgcag
    14701 ggagctttat acatacatgg actctgccaa tatcgattta aaatccttta gtgaagattt
    14761 ttattacaaa attactggtt ctcatttaca acctgttctg gatactctca tctatttgaa
    14821 gaaggaaacc agtgtatggt ttgaaattac taatttggtg attccagaag aaaatgattc
    14881 ggatgccgag tttgagagga tgtctgaatg gattatgact aatctggggc ctgatgttcc
    14941 tttacacttc actgcgtttc atccagattg gaaaatgatg gataaagctc cgactccatt
    15001 ttcaacttta caaagagcca gagaaatcgc aatcaagaca ggcttacatt atgtgtatac
    15061 gggaaatgtt cacgatacaa ccggcagcag cacttattgt ccccattgta aattacctgt
    15121 gattgtaagg gattggtatc gaatattgga ttatcgggtg gatgatactg gacattgctt
    15181 aaattgtcat acgcaaattg caggagtatt taagggagca gttggtcatt ggggcccaag
    15241 aagagtaatg gttaaattag gttgctctta atcttcacat aataaatttt tagtattata
    15301 tgccatgttt ttgtacatgg tgaattaata tgagatttca agattctgac tttgaagaac
    15361 gttataatac aatgtggaat aaaattgcag tgtctgctga tgttcaaata cggcaattat
    15421 ttggagccaa aggatttttt agtgaacagc agcccaatta ctatcaactc cttgcaaact
    15481 atgctcaagc agccaaaaat attgtcgata atttaaatcg tcaatctccc atgtttgatg
    15541 acaaagaata tgttgaaggc tatatgattg ctactctcca atcggtatat aaagattttt
    15601 ctcaatataa accgagaatt gcagggcgtt atggagagca ctcaagctgt gttgaactga
    15661 ttaacaagac cttggattgg gtacaatcct ttgatttaaa attagaaaac ctctctgaat
    15721 ctgatgatga gatgaaaatt actttctaat tgcaagctat cctgtattat gagaaatctg
    15781 taatgcggga tagcaacatt tccctattac atcttactcc tatagatgca ctttgcttgt
    15841 gtggcaccag agcgtcaatg tttattcatc atcaattgtg ctataattga acaatgattg
    15901 cagggaggta tgatgaaaca attggttgtt gcaggatttt tcttattact tctaacttcc
    15961 cctttatcag cgctggatgt tgatacctat cgtagttcac aaaaacaatt caatacagag
    16021 gataaggcgg aacagggtga tgctcaagca cagtttgaat tagggctcaa atatgaaaag
    16081 ggtgatggag taaataaaga tttaaagaaa gcgatttatt ggtatcagaa agctgcagat
    16141 caaggacaag ctgaggcaca aaataatctc ggtgtattgt atcttaaagg agagggtgtg
    16201 ccccaaaaca gtcagcaagc aatgtattgg tttaagaagg cttcggaaca gggtttggca
    16261 attggtcaaa ataatatagg gatcctatat gaaaatggcc tgggtgttaa aaaagatccg
    16321 ggccaggctt ttatctggta tcaaaaggcc gcagaaggcg gaaattcaga cggacaatat
    16381 aaccttgcag tcatgtacat gtatgggaat ggtataccaa aagatatcaa gaaggcgatt
    16441 cactggtata tcaaagcagc agagcaagga gatcttgatg ctcaaaataa tcttggtgtt
    16501 ctttatgagc gaggcgaaga ggttccaaga gatttaaaag cagctattag ttggtatacc
    16561 agagcagcaa atgaaggttc actgattgct caaactaacc ttggtgtatt gtatatgact
    16621 ggtgatcctt ccattcaaga tggcaagaaa gcaatatatt ggtatgaaaa agcagcggca
    16681 caaggtgggg aaaaagctca aaataatctt gggtatattt atgaacaagg aattggtacc
    16741 gaaaaggata tgaaaaaggc tatttactgg tacgaaaaag ccgcagagaa tggatttact
    16801 ctggctcaaa ataatttagg tgttctctat tcaaatgatg gcgagctgca agattacaaa
    16861 aaggcttatt tatggttcaa aaaagccgca gatcaaggat ttgccgaagc ccaaaataac
    16921 ttgggtttaa tgtatatgaa aggtaatgga ttgtcagtta attatcacga agcagtcttg
    16981 tggtacaaga gagcggcaga acaaggatta cctttagcgc aacataatct cgcgattatg
    17041 tatatgaaag gcttggggat aaagaaagac aataaactgg ccattaaatg gtaccaaaaa
    17101 gctgctgaaa aaggattgga tctggcgcaa aacaatttgg cggttatgta tatacgtggt
    17161 gaaggagtaa aaagagattt taagaaggcc atgtattggt atcaaaaggc agctgaacaa
    17221 ggtttggatt tggcacaaat taatctgggt attatgtatc ttgatggcat gggtgttaat
    17281 aaagatttcg ctaaagcaaa gtattggatt ggaaaagcca aggacagtgg ttctcaagat
    17341 gcgctgacca tatggaataa aaataaattg tggaaatatc agtgatcatt ggtgtgggtc
    17401 ttagttgatg tattgtgcaa aatgctcaag tctggttata taattaaaat acgctgaata
    17461 atctaatgga ttagaaaagg atgtctgctg ctcgccaaat caataatccg aaaatgagac
    17521 aatggcgaat attgttggtt tacaatattt acagaatagt aagtattttt ctatttttag
    17581 gcatttatta tttcagttat atcaataaat attatccctt attatttctc tgtatcgctt
    17641 ttgcctattt agtatttgct ttgatttttc tttatttttg gcatagaaaa attttaagtt
    17701 ttgataagca ggtgttttta tcgggtacga ttgatgttat agccatttcc agcatgctgg
    17761 gtctcattgg taatttggaa tccggttatg gcatattatt aaatgtgact attgctgctt
    17821 taagcatttt agttcccggg cggttggcca tctattttgc atcactggcc agttgtttat
    17881 tgctatgtgg gaatgttttg caatttttta tttataatca aaaagactta agcacttttt
    17941 tttatagtgg aatctatggg gctggttttt ttgcaacggc tatcaccgcc tggtatttat
    18001 ccaattgggt cagaatgtct gagagtctgg ccagacaccg tagctatgag ttggcaggaa
    18061 tgcaaaggct caatgaatat attgtcgaac gattgcattc aggcattatt tatgttgcag
    18121 agggaaagcg aatccggttg atgaatactg ctgcccgtga attttttaat ctgagtaaac
    18181 atcatgcaat tcaaaaattg gaccaactgt ctgattcact tgtatccaaa tttgatgatt
    18241 ttcttttaaa aacaacgaat aatgaacgta cagctcaaac tattattgaa gatccttatc
    18301 ttagggtaca ttttttttct acttctgttg gacataagtc tgaagtactg attattctgg
    18361 aggacatgac ttatattgca caacaagcac aacaactcaa attagcggct ttagggcgtt
    18421 tttcggcaag tattgctcat gaattacgta atcccttggg cgcgattgct catgctattc
    18481 aattgtttgg agataatgag gatttaaatg aagagaatac tcgtttaaaa caattaatca
    18541 tgaacaattg tgagcgaatg aacagggtga ttaaaaatgt attgcaactt tcaaggcgtc
    18601 agaaatctca accacaggtt aatgaacttg ctacattttt agaacatttt aagcaggatt
    18661 ttactcacca taatccctgt aaccttacga taaaattacc tgccaataag tcgttatcgg
    18721 tggtctttga taaaagtcaa ttggagcaga ttttggttat attgtgtgaa aattccattc
    18781 aacatggacg ggatcatgaa ggtaatgtga atatagtcat tgctgccaaa tcttcttcga
    18841 ataaaatcac tttgacggta tgcgattctg gaccgggtgt acctccagaa cacagagata
    18901 atatctttga accatttttt accaccttgc gaggtggtac tgggatgggg ttatttattg
    18961 ccagagattt gtgtgaaatt aatcaagcca ggctaaattt gttaaactca aataaaggaa
    19021 gctgttttgc gatcactgtg aatccatcgg atgaactttt aatatgatca aaagtaaagt
    19081 gcttgttata gatgacgaac cagacattag agagttatta actcttactt tatcacgcat
    19141 gggtttaatt tgtgatgcgg tgtctgattt taagcagggc atagaacata taaaacaaaa
    19201 ttattattct ttggttttaa ccgatatgcg tttgcctgac ggagatggta tagaaattgt
    19261 taaattcatt cagaaataca aaccacagtt gccagtcgct gtcattactg cttatggaaa
    19321 tgtagaaggt gcggtcaata ctttaaaggc tggtgcattt gattatgtat ccaagcctgt
    19381 tgacttaaag atgctcagag aactcgttaa gacggcatta tccatgcaaa atccgtctac
    19441 cgagagtcct gatgaaggtc tattggttgg tgagagcctg ataatgcaac aactgcgtga
    19501 gagcattcat aaattagctc gtagtcaggc accggttttc attcaaggtg attcgggtgt
    19561 tgggaaagaa cttgttgctc agctgatcca tgctaatggt cccagaaaag ataagccatt
    19621 tattcctgta aattgtggtg ccatacctgg tgagttgatg gagtcagagt tttttggcca
    19681 taaaaaagga agttttacag gagcgatttc ggataaaaca ggattatttg ttgcagctca
    19741 tggcggcact ttgtttcttg atgaaatcgc cgaacttcct ctcgccatgc aggttaaatt
    19801 actacgcgca attcaggaga aggcaattaa accaataggt gagttatatg aaattccggt
    19861 cgatgttcgc attctaagtg cgagtcataa aaatttgatt gaggaaatca atgcagggag
    19921 gtttcgtcaa gatttgtatt atcgaattaa tgtgattgag ctgcgtgttc caactttaat
    19981 ggaaaggctt tctgatatcc cttgcctgac agaggcgata ttaaaaaaat tatctaaaaa
    20041 tcaaaacaga aatttggtta aaataaatcc ctcttgtttt gaaatattga gccgttatca
    20101 tttcccggga aatgtcagag agttggaaaa tatccttgag cgtgccatgg ctatgtgtga
    20161 aggagaggtc atagagccaa gcgatttaca tttgcccaga acaacaataa ccccagctgt
    20221 tgccgtaccc tccacgtcaa ctaacttaaa tggttacctt caggatcagg aaaaagagtt
    20281 aattcttgat gcgcttgaga aaacaaaatg gaacagaacc gctgcggcac aactactggg
    20341 ggtcagcttc cgaactctcc gttatcgatt aaaaaaactg ggacttgatt agatgttgcg
    20401 ctttgaaggt tttcaaaggt taaaatagac ctcttgcctg gccagcttct ttcgttttat
    20461 tgtcgccctg aaaacgtttc tgagttgctc atttactgta tcaggactta tgccccccaa
    20521 tctcta
//
LOCUS       DACTJE010000041        20478 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.041, whole genome shotgun sequence.
ACCESSION   DACTJE010000041 DACTJE010000000
VERSION     DACTJE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20478
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.041"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            74..439
                     /locus_tag="I9760_001186"
     CDS             74..439
                     /locus_tag="I9760_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042962.1"
                     /translation="MLPSPFPVYVIEGSAVVNHPYPGAKKVLLPTDNGYVDYPGCYIA
                     CYSHNTGVYAISPTISVMGQIRVKGQYDARICQPDGYKNQDISAAYQFKQLCAEKIPA
                     CQNNSCWAGGDTGGWFGIQ"
     gene            complement(540..1553)
                     /locus_tag="I9760_001187"
     CDS             complement(540..1553)
                     /locus_tag="I9760_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0716 family Dot/Icm T4SS effector"
                     /protein_id="HAT5042963.1"
                     /translation="MFDDTNMSKNESGTAKPIIKSTLMFFNSKLTDLNDKRLQLNRGK
                     GLAKYLAKSQKNTRDLAPLDAALGVMSFSLYMLRFSANVGLLAHLILVESQEKTNHKI
                     RRDIYYSLLNDSLWCVVNLTQFFWLSYRNSISAGLQGMQLETLAQLIDILVMIIRYQQ
                     DKKEYDLKYNQAMGMERSRLAIEWQNKELNGLRSLLTSLSVVIAFGLFSFSGVAVPLS
                     PVISSIILISSLLRVLIDIEKDRQLIHQLKLNETSREQIKNEQLAMTNARMKDLNKII
                     LNSVFLPIGFFLLITTPIPLTIIACVSMLLVHWLITNLINMRYSPNISVSPTIQTAEL
                     SLC"
     gene            1684..2355
                     /locus_tag="I9760_001188"
     CDS             1684..2355
                     /locus_tag="I9760_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HAT5042964.1"
                     /translation="MRLLIVEDHKRTADFICKGFNEYQFQADAVYDGAEAIYRISEIN
                     YDAVVLDVMLPIMDGWAVLEKIRELKPDLPVLMLSACDDVDSRVKGLEMGAYDYLVKP
                     FSFNELLARVKSLLRRKPKESPILLKINDLTLNLQKHTAIRGDKKLPLTAKEFMLLTF
                     MLKRSGEVLSRTVIAEQVWDINFDTNTNIIDVAIKRLREKVDNDHPTKLIHTVRGVGY
                     VLEIR"
     gene            2336..3433
                     /locus_tag="I9760_001189"
     CDS             2336..3433
                     /locus_tag="I9760_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAT5042965.1"
                     /translation="MYWRFANSMSITNRLLLIFIGSTVLILSIITGLIYPPMKELLHH
                     SHLNNEHYNYLLTRICIKNFFVGLWFSTLIIILASYLLAKKSMRPIKIFTKELASINA
                     SSLEKRLPYTGNPKELEELALTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRTA
                     TEITLTKPQTIETYQHVLQTHLEEYQSLTQLIDNLLFLTRCEHGQLQLNVRTLSANHL
                     ISSIIEYYYSIAQEKGIEIQMFGEAQIEVDEHLFKRVISNLIDNSLTYTNKGGRILII
                     IEKRTDNSIQIRIKDNGIGISKEHLPLLYHGFYRVDYSTNKENAGLGLGLAIAKSIME
                     HHKGQLAIDSKMGLGTTVILTLPGKSFLKIE"
     gene            complement(3575..5575)
                     /locus_tag="I9760_001190"
     CDS             complement(3575..5575)
                     /locus_tag="I9760_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HAT5042966.1"
                     /translation="MSDTPFIAANKKVAELTFRVIILAIILTVLLAMSNAYLALKLGI
                     LTSASIPAAIISMGILRFFKNSTILENNAVQTAASAGEAVAGGIVYTIPALIIIGFWN
                     HFDYVTNFFIAVCGGILGVLFSIPLRRILVNDQTLKFPEGRAIAEVLKSSADKVGIKD
                     IFLGGLVGGLLELLQIGFKVIASSWNYWFVVKRSLFGLGAGFSATMIGAGYLVGHDMA
                     ISIFLGAVISWLIALPIASQFYPDFLNQYPAEQAATLLWNSEMRYLGIGAMLFAGIWT
                     FIKLAKPLTRSVKTSFRTYISKSKFREQYPRTEKDIPMPFILMGIGLMAGILFLFFQI
                     IFPLGQVGLDNDFSPTIVFIGVIYVLVVGFLFSVITAYFSGMVGVTASPGSSVVISGM
                     LFAAWLLVSAINHLLPLPLTSHQIQAAEAITIIIGAVVTGIAAIANDNTQDLKVGQLV
                     GATPWKQEVMLLLGVVISALVIPPVMQILFNVYGIAGVMPHAGMDVTQSLPAPTAALM
                     AAITEAVFRNSLPWNMMLAGSCIILLAIFLNRILNIKRFLNLSILGIAIGMYLPLSSS
                     VPLFIGGMIALFVQNRLGRLKVNPEESTNRKQKGTLIACGLVAGSAIMDVLLAIPFSI
                     FHSPDALQLVGSSWKQYGVYLGVFSTLLLAWWISHRVCRSKI"
     gene            complement(5575..6270)
                     /locus_tag="I9760_001191"
     CDS             complement(5575..6270)
                     /locus_tag="I9760_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAT5042967.1"
                     /translation="MPIQMNLINKIGLMFGLLFYSFLASAITTEVIKKETADYLLDIK
                     YPQGFQSNGVNQAVKEFITNQQKSFMNELSEDADTPADAPGKTGLNITYAIPYQSTNA
                     LSVRFNISIYHRGAAHPSNAVKVLNFIKNQPVQLSDLFVPGSDYLQPIADLAKKEITA
                     KKISDENWIKEGTKPTQENYSIWHFTKKGIAIVFNTYQVAAYVYGEQTVDIPLSLISA
                     MVKPEISKAVWGN"
     gene            complement(6297..7508)
                     /locus_tag="I9760_001192"
     CDS             complement(6297..7508)
                     /locus_tag="I9760_001192"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027223834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HAT5042968.1"
                     /translation="MFACDELLLNASTIDATGLQLSNQAIVIKKGRIEWCGSEDQLPA
                     HFQESAKSRKDCHGQLITPGLIDCHTHLVYAGHRAAEFRLKLQGVSYADIAKSGGGIL
                     STVQMTRDASEEELIDQSLPRLLALKNEGVTTVEIKSGYGLDLQNELKMLKVARQLGE
                     MAGVRVKTTFLGAHAVGPEFKGNSQAYVDFLCNEMLPAAKNMDLVDTVDVFCESIAFS
                     IRQAEQIFQAAKDLNLPIKCHAEQLSNMGASSLAARYGALSCDHLEFLDENGALNMVK
                     ANTVAVLLPGAFYFLKEKQKPPVDLLRQVGVGMAIATDSNPGSSPTTSLLLMMSMACQ
                     FFSMSIPEVLSAVTYQASRALGMEKDIGSIEAGKIADLVLWSIKDSAALCYYFAYPLP
                     HQTMVAGEWVS"
     gene            complement(7508..8317)
                     /locus_tag="I9760_001193"
     CDS             complement(7508..8317)
                     /locus_tag="I9760_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAT5042969.1"
                     /translation="MSGIISPIVFYIDEEIVYLITGGGSGIGRALAFALAKRNKQVFI
                     VGRREKLLQETASLSANIHYLCADVSTPQGRELIKASLSTTPQIDGLINNAGTLEPTL
                     LLKDISFAEWHQALNTNIDAALFLPQLLYDKLSGGRVLNVGSAAAYFPIKGWGAYCVS
                     KAALAMLTRCWQIECDSLAFASVMPGIIDTHMQAIARNGINLDPEQVNFYRRLKDHNR
                     LISPETVAEFFIWLLLDIDNETYVSKEWDIYDTTHHSAWLKPPHQVLHWEF"
     gene            8608..9567
                     /locus_tag="I9760_001194"
     CDS             8608..9567
                     /locus_tag="I9760_001194"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HAT5042970.1"
                     /translation="MFDDLSNYRPANPALWQGRKDTTNQERFFQKITFIDNQNELMTK
                     DKKTIFLGFASDAGIKRNLGRTGAKLGPDQIKTQLAKLPCHNNKHYVDLGNVVCENDE
                     LELAQSQFAQIVHFCHENGHQICAFGGGHEIAWAHYQGLSSLYPKLGVINFDAHFDLR
                     PYKKGEFGNSGTPFSQIATYCEEKKMPFHYCCIGVQKFGNTPSLFEKAKELNVSYLSA
                     EDLYEQSQAWQIAFLDDFILNLDHIYLTICLDVLAECYAPGVSAPQALGLSPWQIMPL
                     LKYLIQSGKVVSLDIAELSPPLDSELKTARLAALIIAELLDTN"
     gene            9580..10554
                     /locus_tag="I9760_001195"
     CDS             9580..10554
                     /locus_tag="I9760_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmL"
                     /protein_id="HAT5042971.1"
                     /translation="MKKTVLWGALFTLITTQANSEATQQAISTQSSASTGTNVVSEST
                     NQVPATNQNTQQNQLNQPVQSNGQQPTQTIQNNQPNQPSQTGEQQSNQNTQNNQQNQP
                     AQQQTTQQPAQQQVPSQQTAPVINCDYKISAETKAIEQSLVLTWSEKAIIQSFDFDPA
                     TVDAQLQKLQACFTDQGWLSFNSALQKSGNLDAIKSQKLHVSSQIDGQPQVTEAIENQ
                     WKIIIPLQVVYQNDKEKVTQLLNVNVTVGRKITGDLGINQMIAVPRTATNTSQTNSPS
                     NTNPADANTSTPQTPGDTTNNATQTNTQTQTGTPTNSPPSGSTTNTQN"
     gene            10611..11783
                     /locus_tag="I9760_001196"
     CDS             10611..11783
                     /locus_tag="I9760_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042972.1"
                     /translation="MRLIFTGVLCLLTLNAQANVIQYFAGISYNNPADLFKVKNGILL
                     IGGTGSYADLQFKGSALNFNTFQYESGVNHSRTYIVWPYGRVAKRLNDKTVVAVDLTE
                     PFNSNLDWGNDAFTRYAATQNYLTDVDLSPKISYAISKKLQIGGGINFNVLLKNEVNW
                     AFPTGQSTYANLINRSSSFGVGYNLGINYAVNDTNFLGITYYSRIRQNTSGTSYLGLA
                     ANPDFQFGFYMPATTVASYVHIFNPKWLINLQVFQSEWNANQKVRLYNTAAPPPFTNF
                     IFDMHFDASYAYLAAIRKQVSDKLGIALAGMIDDGPEEDGLRTIVFPSDTQYFLGLIG
                     DYRFTEHASMELILGHVYSNPSIQNKAKVNNVPVPFTTGRVTINANVLDLKVKIEG"
     gene            complement(11823..12248)
                     /locus_tag="I9760_001197"
     CDS             complement(11823..12248)
                     /locus_tag="I9760_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAT5042973.1"
                     /translation="MYNIMSFLCGLLFGAGLTISNMINPNKVLNFLDINGFWDPSLLI
                     VMAVAVTVTFLGYKLINKFDKPILCNQFYLPEKKNIEKKLILGSIIFGIGWGVAGYCP
                     GPSITALTTFNSDPVYFVIGLIAGSWTYYWLFKKRTIQK"
     gene            complement(12248..12685)
                     /locus_tag="I9760_001198"
     CDS             complement(12248..12685)
                     /locus_tag="I9760_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HAT5042974.1"
                     /translation="MDYITSFTPYLSAIGGLLIGLSAVLFLWLNGRIAGISGMIHGLF
                     PPEKPFPAWRIAFLLGLAAAGLIYFISPVIQFPLRTHYPVFLLLISGFLVGFGTRMGQ
                     GCTSGHGVCGLARFSKRSFIATLLFILSAMITVYLIRHVGGLY"
     gene            complement(12795..13406)
                     /locus_tag="I9760_001199"
     CDS             complement(12795..13406)
                     /locus_tag="I9760_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAT5042975.1"
                     /translation="MKKIYWAVILMCLGLTSCVEFDESTLITDDAGYVHRTVHYLRDK
                     RGRNYFPMQIKAPGERLFVFDPKASAWAAYDEEGHRVMTGAGSGGIDVCEENPNQSCR
                     TITGTFRIYNKRGIDCRSGEYPVDTKGGAKMPYCMYFYRGFTIHAAYEVPEHPSSHGC
                     VRVFPSAAKWLNEEFLRVGTKVIVLAYPDQNGVKTMSSLEKVN"
     gene            complement(13448..15121)
                     /gene="ettA"
                     /locus_tag="I9760_001200"
     CDS             complement(13448..15121)
                     /gene="ettA"
                     /locus_tag="I9760_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAT5042976.1"
                     /translation="MAQYIFTMNRVSKIVENQRFILKDISLSFFPGAKIGVLGLNGSG
                     KSTLLRIMAGVDTQYEGEARPQPGIKIGYLAQEPELDLNKTVREIVEEGVAEIKEKLS
                     RFDAISMRFAEPMSDDEMNTLLMEQGELQNEIEACGGWDLERKLDVAADALRLPEWDA
                     IIGKLSGGERRRVALCRLLLSSPDMLLLDEPTNHLDAESVAWLEHYLEEFSGTVVAIT
                     HDRYFLDNAAEWILELDRGEGIPYKGNYSSWLEQKEARLSMEKKQEDALQRAIKAELE
                     WVRTSPKGRHAKNKARLARFEEMNSKEFQKRNETNEIYIPPGERLGDLVLEGEKICKS
                     YGDRILIDNFDFKLPKGGILGIIGPNGAGKSTFLKMLTGQEEPDKGVIRIGETVKLAY
                     VDQMRDNLNPNNSVWQEISDGHDIMQIGNFQMPSRAYVGRFNFKGTDQQKKISQLSGG
                     ERNRVHLAKLLKSGGNVLLLDEPSNDLDVETLRALEEAILNFPGCAIVISHDRWFLDR
                     ICTHLMAFEGDSQITFFEGNYTEYETDRKRRLGDEANRPSRIKYRKLSD"
     gene            complement(15164..16186)
                     /gene="tdh"
                     /locus_tag="I9760_001201"
     CDS             complement(15164..16186)
                     /gene="tdh"
                     /locus_tag="I9760_001201"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAT5042977.1"
                     /translation="MKSLVKAKKEPGIWMQDIPVPEYGVNDVLIKIKRTAICGTDIHI
                     YSWDEWAQATIPVPMTVGHEFYGEIVEVGKEVQGLKVGQRVSGEGHITCGFCRNCRAG
                     KRHLCRNTLGVGVNRPGCFAEYLALPATNVIALPDNITEEQAAILDPFGNAAHCALAF
                     DVVGEDVLITGAGPIGIMAAAIVRHIGARHVVITDVNDHRLELARQMGVSRAVNVKYQ
                     KLIDVANELGMLEGFDVGLEMSGNPMALNDMMKAMNHGGHVALLGIPPQETPIDWNQV
                     IFKGLVIKGIYGREMFETWYKMIAMLQSGLNISPVITHNFPVDEYQHAFQIMASGQSG
                     KVILNW"
     gene            complement(16273..17457)
                     /locus_tag="I9760_001202"
     CDS             complement(16273..17457)
                     /locus_tag="I9760_001202"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAT5042978.1"
                     /translation="MLERFINYLQNELETLKADGLYKSERVISSQQQADVKVNEREVI
                     NLCANNYLGLANDPELIAEGQAALAKYGYGMASVRFICGTQTPHKQLEQKISHFLSQE
                     DTILYSSCFDANTGLFETLLTEEDAIISDALNHASIIDGVRLCKAARYRYANNDMKAL
                     EEQLIAAKNARFRLIATDGVFSMDGILANLPAICELADKYDAMVMVDDSHAVGFMGKT
                     GRGTPEHFGVSDRIDIVTGTLGKALGGASGGYTASNQVVIDWLRQRSRPYLFSNTLAP
                     VIAHTSCVVLDKLSTNNSLAEKLKRNSHYFREGMTQLGFELIPGEHPIIPVMLGDASL
                     AGRIANRLLDLGVYVVGFSYPVVPKGLARIRTQMSAALELHHLDKALKAFETVGKEFS
                     VI"
     gene            17638..18288
                     /locus_tag="I9760_001203"
     CDS             17638..18288
                     /locus_tag="I9760_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAT5042979.1"
                     /translation="MNHSARINMIKQQLRTGDVLNESILDLYDELLRHEFVPEPFSHF
                     AYSDMQIPLAYGQRMLTPLEEGTILQSLDLKGHETVLEVGTGTGFMTALLSKLCKKVI
                     SIDYYSEFTANAKRKLEEHNCNNVELITGDACRGWLESAPYDVMVFTGAMEKLTDTHK
                     LQILPGGKLFAILGKSPVMKAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF"
     gene            18300..19667
                     /locus_tag="I9760_001204"
     CDS             18300..19667
                     /locus_tag="I9760_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HAT5042980.1"
                     /translation="MRKSLFCWILTLGVSTHVFATDLMDIYQQALENDTIFKEAYDTY
                     MSSTEAIPQARAALYPQVGLGSQAGRNYQDAVAGAFSANQYYGSYLWQVNASQALFNY
                     QAWAKVAQAKASVKAAQATFNDAAQNLILRTAKAYFDVLFAKDTLDFAEAKKRANKRQ
                     YDQATQRFQVGLDAITSVYEAKAAYDQSIATVIAARNNQINQSENLRKLTNHVYETLA
                     PLKDSKIPLVKPEPNDVNQWIDTGLKQNYKLYAAKYNLEVAKDNVKAISAGNWPVFSL
                     QSNASQVHNNASGNTVFIPSKQTQANIAIAMNFPVFQGGLVQAQTRQAQYGFQSTSEK
                     LEQTYRDVIVNSRIAFNTITDGISKVKADRQTVISVQNSLQSTEAQFEVGTRTMVDVV
                     NAQQRLFEAQEQLARDQYDLINSILTLKYLAGTLNVNDLEQINSWLATTRVNGFSPVD
                     NKTSK"
     gene            19685..>20478
                     /gene="ttcA"
                     /locus_tag="I9760_001205"
     CDS             19685..>20478
                     /gene="ttcA"
                     /locus_tag="I9760_001205"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAT5042981.1"
                     /translation="MSSNPSSVEKKLLHYTGKAIADFNMIQRGDRVMVCLSGGKDSFT
                     LLTILNQLRIKSGNKFEIFAFTLDQAQPGWNDACLRQWLAEKSIPHEILTRDTYSIVK
                     EKIPEGKTYCSLCSRLRRGIIYRYAEENGFNKIALGHHRDDLIRTLMMSILYNGDIRS
                     MPPKLLSDNKKHIVIRPLCYVQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLI
                     DQLAIENPKVPSNMLHALQSLKPSQLMDQNFWNFKNLEDGLETAQS"
BASE COUNT         6601 a         4398 c         3609 g         5870 t
ORIGIN      
        1 tccgggtatt ttattaatta cgaactcttt tgcccttgat atacctggga atattaccaa
       61 ttctaacaaa gatctgctcc cttcaccttt tcctgtttac gtcattgagg ggtctgctgt
      121 tgtcaatcat ccttacccgg gagcaaaaaa agtactcctg ccaaccgaca acggctatgt
      181 agattatcca ggatgctata ttgcttgtta ttcgcataat acaggtgtct acgccatttc
      241 cccaacaatt tcagtcatgg gacaaattcg agtcaaaggg caatatgacg cccgaatttg
      301 ccaacctgat ggatataaaa atcaagacat cagtgctgct tatcaattta aacagctttg
      361 tgctgaaaag atccccgcct gccaaaataa cagttgctgg gccggtggcg atactggcgg
      421 atggtttgga attcaataag cagaccaatg aatttcacag aataaaatga gataggatac
      481 ttgattttgg gtcaaagcca cgcctttaaa atttttagca agcatcatca ctctcaactt
      541 cagcagagag ataattcagc agtttgaata gtaggggata cggagatatt tggtgaatac
      601 ctcatattaa ttaaattagt aattaaccag tgaaccaata acatacttac acaggctatt
      661 atagttaaag ggattggagt tgtgatcaac agaaaaaaac caataggtaa aaaaacgcta
      721 ttaagaatta ttttatttaa atctttcata cgagcgttcg tcattgccaa ttgttcattc
      781 ttgatttgtt ctcgactagt ctcattaagc ttaagttgat gtattaattg cctatctttc
      841 tctatatcga ttaaaactcg taaaagtgaa ctaataagaa tgatagaaga tattacaggc
      901 gaaagtggta ctgccactcc tgaaaatgaa aataatccaa aagctatcac tacagataag
      961 cttgtgagca aggatcgaag accatttaat tctttattct gccattcaat tgctaaacga
     1021 gagcgctcca tacccatagc ctgattgtat tttaagtcat attccttttt gtcttgttga
     1081 taacgaataa tcatcactaa aatatcgatg agttgagcca acgtttctaa ttgcatccct
     1141 tgaagacctg cagatattga gtttctataa gataaccaaa aaaactgggt caggttaaca
     1201 acgcaccaca agctatcatt taaaagactg tagtaaatat cccttcgtat tttatgatta
     1261 gttttttctt gcgattcgac taaaatgaga tgcgctaata aaccaacgtt agctgaaaat
     1321 cgaagcatat acagagaaaa gctcatcaca cccaaagccg catctaaggg agcaagatcc
     1381 cttgtgtttt tttgtgattt tgctaaatac tttgctaaac ctttaccacg atttaattga
     1441 agtcttttgt catttaaatc agtcagtttg ctattaaaaa acattaatgt ggattttatg
     1501 atcggttttg ctgtgcctga ctcgtttttg ctcatattag tatcatcaaa caattatgaa
     1561 ttaataataa gcaataaaat ctgtcagaag agtgacatca agattacaaa attgtaatct
     1621 aattcattat gataattagc atttttatgt taaaatagaa cataatttaa acacaatagc
     1681 attatgcgat tattaattgt tgaagaccat aaaagaacgg ctgattttat ctgtaaagga
     1741 tttaatgaat atcaatttca ggcagatgct gtatacgatg gtgcagaagc aatctatcga
     1801 atctcagaaa ttaactacga tgctgttgtt ttagacgtta tgttacccat aatggatgga
     1861 tgggctgtac ttgaaaagat tcgtgaacta aaacctgatt tacctgtttt aatgctttct
     1921 gcctgcgacg atgtcgatag tcgtgtgaaa gggcttgaaa tgggtgccta tgattatctt
     1981 gttaaacctt tttccttcaa tgagctcctt gcccgagtaa aatctttact acgaagaaaa
     2041 cccaaagaga gccctatctt attaaagatt aatgatttaa ctctaaactt acaaaagcat
     2101 acagcaatac gaggcgacaa aaagttacct ttaacagcaa aagaattcat gttattgacg
     2161 tttatgttga aacgaagtgg tgaagtgctt tcaaggacag tcattgctga acaagtttgg
     2221 gatattaatt ttgacacgaa taccaatatc attgatgtag cgatcaagcg tttaagggaa
     2281 aaagtggata atgatcatcc tactaaattg atccataccg tcagaggagt aggctatgta
     2341 ttggagattc gctaactcaa tgtccattac taaccgctta ttactgatat ttattggttc
     2401 aaccgtactg atattgtcca ttatcactgg gttaatctat cctccaatga aagaattact
     2461 ccatcatagt catttgaata atgagcatta taattattta ttaacacgga tttgtattaa
     2521 aaatttcttt gttggactct ggttttctac tttgataatc attcttgcca gttatttgct
     2581 cgcaaaaaaa agcatgagac caattaaaat attcactaaa gagcttgcat cgattaatgc
     2641 ctcatcacta gagaaacgat taccttacac agggaatccc aaagaactgg aagaattagc
     2701 tttgacttgt aatgatatgc tctttagaat agaaagtgca tttagccata taaagcaatt
     2761 ttcagccagt atagctcatg aattacgaaa tcccattcat tacctccgaa cagctaccga
     2821 aattaccctg actaaaccac aaacgattga aacctaccaa catgtattac aaacgcatct
     2881 cgaagaatat caaagtttaa cccaacttat agataatctg ctctttttaa ctcgctgtga
     2941 gcacggccaa cttcaattaa atgttagaac attatctgca aaccacttaa tcagctcaat
     3001 tatcgagtat tattattcaa tagctcaaga aaagggcata gaaattcaga tgtttggcga
     3061 ggctcagatt gaagtagatg aacatttatt taagcgcgtt atttcaaatt taatagacaa
     3121 cagtctgacc tataccaaca agggcggaag aatactaatc atcatagaaa aaagaacaga
     3181 caacagcatt caaatccgta taaaagacaa tggaattggt atatcaaaag aacacttacc
     3241 tcttttatat cacggatttt atcgcgtaga ttatagcact aacaaagaaa acgcaggtct
     3301 tggtttaggt ttggctatcg ctaaatcaat catggaacac cataaaggcc agcttgcgat
     3361 agatagcaag atgggtttag gcaccactgt tatattaacc ctaccaggaa aaagcttttt
     3421 aaagattgaa taatgtatgt atttcatcta tccgagacct taagggaacg gatatacatt
     3481 gaactaagtt attctaagaa catagacttt tcccgcctca cacagcaaca ccaatctaca
     3541 ataagtagaa ttgttagagt aattatcaat taactcaaat tttgcttctg cagacacgat
     3601 gacttatcca ccatgctaaa agcaaagtag aaaacactcc taaatacact ccatattgtt
     3661 tccatgatga tcctaccagc tgcaaagcat cagggctatg aaaaatagaa aacggtatag
     3721 ccagtaacac atccatgatc gctgatcctg ccactaaacc acaggcaata agagtgcctt
     3781 tttgctttct attggtagat tcttcagggt taactttcaa ccgtcctaaa cgattttgaa
     3841 caaatagggc aatcattcct ccaataaata aaggaaccga agaggaaaga ggtaaataca
     3901 ttccaatggc aatacctaaa atagataaat taagaaagcg tttgatgttt aatatgcgat
     3961 tgagaaaaat agccaataaa ataatacatg aaccagccaa catcatattc cagggcaatg
     4021 aatttctaaa tactgcttcc gtgatggctg ccattaatgc ggcggtcggg gcgggtaacg
     4081 attgtgttac atccattcca gcatgtggca ttactcctgc tatgccatag acattaaata
     4141 aaatctgcat gactggagga ataaccaaag cagaaataac aacgccaagc agcaacatca
     4201 cctcctgttt ccatggagta gccccaacta attgacctac ttttaagtct tgggtgttgt
     4261 cgttagcgat agccgctatg cctgtgacta cagcacctat aataatagta atagcttctg
     4321 cagcttgaat ttgatgtgaa gttaatggaa ggggcaatag atgattaatt gctgaaacca
     4381 ataaccatgc ggcaaaaagc atacccgaga taaccactga actcccggga cttgcagtga
     4441 ctcctaccat tcctgaaaaa taagctgtaa tcactgagaa taaaaaaccg acgactaaaa
     4501 cataaataac accaataaat acaatagttg gtgaaaaatc attatccagt ccaacctgcc
     4561 ctaatggaaa aatgatttga aagaacaaaa acagtattcc tgccatcagt ccaattccca
     4621 tcaatataaa tggcatggga atatcttttt ctgtgcgagg atattgttct ctgaacttgc
     4681 tcttggaaat ataagttctg aatgatgtct tgacacttct ggtgagtggt ttcgctaatt
     4741 ttataaaagt ccaaataccg gcaaacagca tggctcctat ccccaaatag cgcatttcac
     4801 tattccataa caaagtagct gcttgttcgg cgggatattg atttaaaaaa tcaggataaa
     4861 actggctggc tattggcaaa gcgatcaacc atgaaatgac cgccccgaga aaaatgctta
     4921 ttgccatatc gtgaccaacg aggtagcccg cacctatcat ggttgcagag aagccagcac
     4981 ccaaaccaaa tagagaacgt tttacaacaa accaataatt ccagctgctg gcgattactt
     5041 tgaaaccaat ttgcagtaac tcgagaagtc cgcctacaag cccgccgaga aaaatgtctt
     5101 taataccaac cttgtctgcc gaagatttca agacctctgc aatcgctctt ccttcaggaa
     5161 atttgagggt ttggtcatta acaagaattc gacgcaaagg aatagaaaac aaaactccca
     5221 ggattcctcc acaaacagca ataaaaaaat tagtgacgta atcaaagtgg ttccaaaacc
     5281 cgatgataat gagcgcagga atagtgtaaa cgatacctcc tgcaacagcc tcaccagcag
     5341 atgctgctgt ttgtacggca ttgttttcaa gaatggttga gtttttaaag aatcgcaaaa
     5401 tccccatgga aataatagcg gcgggaattg aagcggaagt taatataccg agtttaagcg
     5461 ccagataagc attagacatg gccagtagaa cggtcaaaat tatcgccaga ataatcactc
     5521 taaaagtgag ttcagctact tttttattcg ctgcaataaa tggagtatca gacatcaatt
     5581 accccacact gctttggaaa tttctggttt taccatagca gaaattagtg ataatgggat
     5641 atccactgtt tgctctccat aaacataagc tgccacctgg tatgtattaa aaacaatagc
     5701 aatccctttt ttggtaaaat gccaaatact ataattttcc tgtgtcggtt tcgttccttc
     5761 tttaatccag ttttcatctg aaatcttctt ggctgtgatt tcttttttag ccagatcagc
     5821 gattggctgc aaataatcgg aacctggaac aaataaatca gacaattgta caggctgatt
     5881 cttgataaag ttgagtacct ttactgcatt tgagggatga gcagctcctc gatgatatat
     5941 ggaaatatta aatcgaacac ttagggcatt agttgattgg taaggaatag catatgtaat
     6001 atttaaaccg gttttacctg gagcatctgc aggagtatca gcatcttcag ataattcatt
     6061 cataaaactc ttttgctgat tagtaataaa ttcctttacc gcctgattta ctccgttact
     6121 ttgaaatccc tgagggtatt ttatatccag caaataatcc gctgtttctt ttttaataac
     6181 ttctgttgta atcgcagaag caagaaacga ataaaataat agcccaaaca tcaggccaat
     6241 tttattgatt aaattcattt gtatcggcat tgtttatctc caatcaagta atcctctcag
     6301 gatacccatt caccagccac catcgtttga tgaggtaacg gataagcgaa ataataacat
     6361 agtgcagcgc tatcttttat agaccataaa accaaatcag caatcttgcc tgcttcaatg
     6421 ctcccaatat ccttttccat tcctaaagct ctggaagcct gatacgttac tgcagataaa
     6481 acttcaggta tagacataga gaaaaattgg caagccatac tcatcatcaa taataaagaa
     6541 gtcgttggag aagaacccgg gttagaatcc gtggcaatgg ccataccaac accaacctga
     6601 cgcaataaat caacaggtgg tttttgtttt tctttaagga aataaaaagc gccaggaagt
     6661 aagacagcaa ccgtattggc ttttaccata tttaacgcgc cattttcatc caaaaactcc
     6721 agatgatcac aggataacgc accataacga gcagccaaag aactggcccc catattagat
     6781 aattgttcag catggcattt tattggtaaa tttaaatcct tagcagcttg aaaaatttgc
     6841 tcagcctgcc ttatagaaaa agctatggat tcacaaaaaa catccacggt atctaccaaa
     6901 tccatatttt tagctgcagg caacatttca ttacaaagaa aatcgacata agcctgacta
     6961 tttcctttaa actcgggacc tacagcatgc gcccccagaa aagttgtttt taccctgact
     7021 ccagccattt cccctaactg cctggcaacc ttgagcattt tcaactcatt ttgtaaatca
     7081 aggccgtagc ctgacttaat ttcaacagta gtaacccctt cattttttag cgcaagaagt
     7141 cttggcaatg attgatcaat taactcttct tcggaggcat ctcgtgtcat ttgtactgtg
     7201 gataatatgc ccccgcctga tttggcgata tcagcgtaac tgacgccttg caattttaat
     7261 cgaaactctg cggctctgtg gcctgcataa accagatgag tatgacaatc aattaatcct
     7321 ggtgtaatta attggccgtg acaatccttt cttgacttgg cactttcctg aaaatgggca
     7381 ggtaactggt cctcagaacc acaccactct attctgcctt tttttataac aatggcttga
     7441 ttagaaagtt gcaaccctgt ggcatcgatt gtgcttgcat tgagcaataa ttcatcacag
     7501 gcaaacatta aaattcccaa tgaagtacct ggtgaggcgg cttcaaccag gcagagtggt
     7561 gagtcgtgtc atatatatcc cactctttgg atacatacgt ttcattatca atatctaaca
     7621 gcagccaaat aaaaaattct gctactgttt cgggtgatat caaacgatta tgatctttta
     7681 aacgcctata aaaattgacc tgttccggat caagattaat cccgtttcgg gctattgcct
     7741 gcatatgagt gtcgataatt ccaggcatta cgctggcaaa ggccagtgaa tcgcactcaa
     7801 tctgccagca tcgtgttaac atagccaagg ccgctttcga tacacaataa gctccccatc
     7861 ctttgatagg aaaataagcg gcagctgaac ctacattcaa tactctgcca cctgacaatt
     7921 tatcatacaa aagttgtgga agaaaaagtg ccgcatctat attggtgttt aatgcctggt
     7981 gccactcggc aaaagaaata tcttttaaaa gcagtgtagg ctccagagtt cctgcattgt
     8041 ttatcaaacc atcaatttga ggtgtggtac ttaaactggc tttgattaac tctctgcctt
     8101 gaggagtgga gacatcggca cataaataat gaatgtttgc agacaaagaa gcggtttctt
     8161 gcagtaattt ttccctgcgc cccactatga acacctgttt attgcgtttt gccaaagcaa
     8221 acgccaaagc tcttcctatg ccgctaccac caccagtaat gagatacaca atttcctcat
     8281 caatatagaa aacaataggt gaaataatac cagacattag acttcttgca taacctgcac
     8341 gttttatttt agaacaagaa aaaagcgact gaacggtcta agtttattaa aagtaaatga
     8401 aaatcttgag tatttagcat ttctcttgga aactagccct atcattagag ctcttgcata
     8461 acctgattat tttgttttag ggcaaggcgc aaacgttttg aggagcggag tttacatgca
     8521 gtaaattagc accacagaaa cgtttgcaac gcggcaataa cactaaagaa acaggttatg
     8581 caagaggtct attaaaagga gaacttgatg tttgacgatt taagtaacta tcggccagcc
     8641 aaccctgcgc tttggcaagg caggaaggac accaccaacc aagaacgttt ttttcaaaaa
     8701 attactttta ttgataatca aaatgaatta atgactaaag acaaaaaaac tatatttctt
     8761 ggatttgcca gcgatgccgg aattaaacgc aatttaggca gaacaggagc aaaattaggc
     8821 cctgatcaaa ttaaaaccca acttgccaaa ttaccatgcc ataataataa acattatgtt
     8881 gatcttggaa acgtggtatg tgaaaacgat gaattggaat tagcccaatc tcaatttgct
     8941 caaatagttc atttttgcca tgaaaatggc catcagatct gtgctttcgg cggtgggcat
     9001 gaaatagcct gggcgcacta tcaaggatta tcctcactct atccgaagtt aggggttatc
     9061 aactttgatg ctcattttga tttacgccct tataaaaaag gtgagtttgg caactcggga
     9121 actcctttct ctcaaattgc cacctattgc gaagaaaaaa aaatgccttt tcattattgt
     9181 tgtattgggg tgcaaaaatt tggcaatacc ccttctcttt ttgaaaaagc aaaagaatta
     9241 aatgtcagtt acctctcagc agaagacctt tatgagcaaa gccaggcttg gcaaattgca
     9301 tttcttgatg attttatcct taatctggac catatttact taacgatatg cctggatgtt
     9361 ctcgccgaat gctatgcccc tggtgtaagc gccccgcaag cattaggttt atctccctgg
     9421 caaataatgc ccctgttgaa ataccttata caaagtggca aagtagtaag tttagatatt
     9481 gcagaacttt cgccgccact agattccgaa ttaaaaactg ctagactagc ggcacttatt
     9541 atcgcagaat tgttagatac taattgagga gtatatttca tgaagaaaac tgtgctgtgg
     9601 ggtgctttat ttactctaat cactactcaa gcgaactctg aagcaactca gcaagcgata
     9661 tcaactcaaa gttcggcgtc caccggcact aatgtagttt ctgagtcaac gaatcaggta
     9721 cctgccacga atcaaaacac gcagcaaaat cagctgaacc aacctgtaca gtcaaatgga
     9781 caacaaccaa ctcaaaccat tcaaaacaat caaccaaatc aaccgagcca gacaggggaa
     9841 caacagtcta accaaaacac ccagaataat cagcagaatc aaccagcaca gcaacaaacc
     9901 acacagcaac cagctcaaca acaagtacct tcacaacaaa cagcgccagt tattaattgt
     9961 gattacaaga tttccgccga aacaaaagca atagagcaat ccctggtttt aacctggtca
    10021 gagaaagcaa taattcagtc atttgatttt gatccggcta ctgtagatgc ccaattgcaa
    10081 aaattacaag cctgctttac agatcaagga tggctaagct ttaattcagc attgcaaaaa
    10141 tcaggtaatc tcgatgccat taaatcacaa aagttgcatg ttagcagcca aattgatggt
    10201 cagccacaag tgactgaagc aatagaaaat caatggaaaa ttatcatacc attacaagtt
    10261 gtctatcaaa atgacaagga gaaagtaacc caattactta atgtcaacgt aactgttgga
    10321 cgaaaaatta ccggtgattt aggcatcaac caaatgatcg ctgtaccccg cacagcaacc
    10381 aatacctcac aaaccaattc accgtctaat acaaacccag cagacgcaaa tacctcgacc
    10441 cctcaaacac ctggtgacac aacaaataat gctacccaaa ccaatacaca aacacagact
    10501 gggacaccaa ccaactcacc tccgagtggc tccacaacaa atacccagaa ttaaattcaa
    10561 caattagtgc ggcattccat taagaatgct gccaaaatca cggaaatatc atgcgattaa
    10621 tatttacagg agtattatgc ctgttgactc tcaatgccca ggcgaatgtg atacagtatt
    10681 ttgcaggaat tagttataac aaccctgctg acttattcaa agtaaaaaat ggtatcttgc
    10741 ttattggagg aaccggttct tatgctgact tgcaatttaa gggaagcgcc ttaaatttta
    10801 atacgtttca atacgaatca ggagttaatc actcgcgtac ttatattgtt tggccatatg
    10861 gtcgagttgc caagcgcctc aatgataaga ctgtagttgc agtagatctt acagaacctt
    10921 ttaattccaa tcttgattgg ggcaacgacg cttttacaag atatgctgcg acccaaaatt
    10981 acctgaccga tgtggatcta agtcccaaaa tatcctatgc gattagcaaa aaattacaaa
    11041 ttggtggtgg cataaacttt aatgtactac taaagaatga agtcaattgg gcttttccta
    11101 ctggccaatc cacttatgcg aatttaatta accgatcctc tagttttggt gtgggttata
    11161 atttggggat taattatgca gtgaatgata ccaatttcct tggtatcact tattattcaa
    11221 gaattcgcca aaatacttcc ggtaccagtt atctgggatt agctgccaac cctgatttcc
    11281 aattcggttt ttatatgcca gcaacaacgg ttgccagtta tgtccatatt tttaatccca
    11341 aatggctcat caacctacag gtttttcaaa gcgaatggaa cgccaatcaa aaagtgagac
    11401 tttataacac agcagcacca ccaccattta ctaattttat ttttgatatg cattttgatg
    11461 cctcttatgc ttatcttgcc gcgatacgta aacaagtctc tgataaatta ggcattgcgc
    11521 tggcaggcat gatcgatgat ggtcctgaag aagatggcct tagaaccatc gttttccctt
    11581 ctgataccca atattttctt ggattgattg gtgactaccg ttttacggaa catgcttcca
    11641 tggaacttat cttgggacat gtgtattcta atcccagcat tcaaaacaaa gccaaggtaa
    11701 acaatgtacc cgttcccttt actacaggcc gggtgacaat taatgccaat gttttggatc
    11761 ttaaagtcaa aatcgagggg tgatcttcca ggtgtaacca cctggcactt cctccaagag
    11821 ttttattttt gaatagtcct ctttttaaat agccaataat aagtccatga tcctgctatc
    11881 agcccaatca caaaatatac cggatcggaa ttaaaggtag tcaaggcagt aatagatggg
    11941 cctgggcagt atccagccac accccaacca attccaaaaa tgatacttcc caagattaat
    12001 tttttctcaa tgtttttctt ctcgggtaaa taaaattgat tacacaatat tggtttatca
    12061 aatttgttta tcagtttgta ccccaaaaaa gttacggtga cagcaacagc cattacaatc
    12121 agtaaactcg gatcccaaaa tccattgata tcaagaaaat ttaatacctt gttaggattg
    12181 atcatatttg aaatagtcaa cccggctcca aataacaaac cacatagaaa tgacatgatg
    12241 ttatacatta atataatcct cccacatgtc ttatcagata cacagtaatc attgcagata
    12301 agataaaaag cagtgtcgct ataaaggagc gcttggaaaa tctggccagt ccacataccc
    12361 cgtgtcccga agtacaacct tggcccattc gggttccaaa acccaccaaa aaaccactaa
    12421 ttagtaatag gaaaaccgga taatgtgttc ttaaaggaaa ctggataaca gggcttatga
    12481 aatagatcaa tcctgcagct gccaaaccaa gtaaaaaggc aatacgccag gctgggaatg
    12541 gtttttcggg tggaaagagt ccgtgtatca ttccactgat acctgctata cggccattta
    12601 accataaaaa aaggacagca cttaagccaa tcagtaatcc accaatggca cttagataag
    12661 gagtaaaaga ggttatataa tccattattt atatttccgt ttcatttaca atgaataaca
    12721 cctgaattga gacactttaa aaaattaaag tgtctctcaa attacattga tgttttaaaa
    12781 caatagtcca aattttaatt cactttttcc aatgaggaca tggttttcac cccgttttga
    12841 tcagggtagg ctaatacgat taccttcgtt ccaactcgca agaactcttc gttcagccat
    12901 ttggcagcac tggggaaaac ccttacacat ccatgacttg atgggtgctc tggaacttca
    12961 taagccgcgt gaatagtgaa ccccctgtaa aaatacatgc aatagggcat tttagccccg
    13021 cctttcgtat cgacagggta ttcacccgaa cgacagtcaa tacctcgttt gttataaatc
    13081 ctgaatgttc cggttatcgt cctgcaagat tgatttggat tttcttcgca aacatctatt
    13141 ccaccagaac cagctcccgt cattacacga tgaccttctt catcgtaggc agcccaggca
    13201 gacgctttag gatcaaaaac aaataatctt tcgcccggtg ctttaatttg cattgggaaa
    13261 taattacgtc ctcttttatc ccttaaataa tgaacggtcc tatgtacata acccgcatcg
    13321 tcagtaatta atgtcgactc atcaaactca acgcacgaag ttaatccaag gcacattaaa
    13381 ataactgccc aataaatttt tttcatttag attttatctc cacaatagta aaacctggtg
    13441 gccttaatta atcgcttaat tttcgatact taattcgtga tggtctgtta gcttcatcac
    13501 ccaatcgacg ctttctatcc gtttcgtatt cagtgtaatt accttcgaaa aaggttattt
    13561 gtgaatctcc ttcaaaagcc atcaaatgag tacaaattct atctaaaaac catctatcgt
    13621 gagaaatcac tatagcacaa cctggaaaat taaggatggc ctcttccaaa gctctcaatg
    13681 tctcaacatc caagtcatta ctgggttcat cgagtagcaa aacattcccg ccacttttca
    13741 ataattttgc caaatgaacc cggttacgct ccccgccaga caattgagaa attttctttt
    13801 gctgatccgt ccctttaaaa ttaaaacgcc caacataggc tcgtgaaggc atttggaaat
    13861 tacctatctg cataatgtca tggccatctg aaatttcttg ccacaccgaa ttgttggggt
    13921 ttagattgtc ccgcatctga tcaacataag ccaacttcac tgtttctcca atacgaatga
    13981 cgcccttatc tggttcttcc tgcccagtta acatttttaa aaaagtagac ttacctgccc
    14041 cattgggacc aataattcct aaaataccgc ctttgggaag tttgaaatca aaattgtcga
    14101 ttaatatccg gtcaccgtaa gatttgcata ttttttcacc ttccagaacc aaatcaccta
    14161 accgctcacc gggcgggata tatatttcat tggtttcatt acgcttttgg aattctttag
    14221 aattcatttc ttcaaaacgg gccaagcggg ctttattttt agcatgacgt cctttaggtg
    14281 aggttctaac ccattccaac tctgctttaa tagcacgttg caaagcatcc tcttgctttt
    14341 tctccatact caaccgtgct tctttttgtt caagccagga actgtagttc cccttatacg
    14401 ggataccttc gccccgatcc aactctaaaa tccactccgc agcattatcc aggaaatacc
    14461 ggtcatgggt aattgcaaca acggtaccag aaaactcttc aagataatgt tctaaccagg
    14521 caacactctc tgcatccaaa tggttggtag gttcatcaag aagcaacata tcaggacttg
    14581 atagcaacaa tcgacaaaga gcaacacgtc tgcgttcacc acccgacagt tttcctatta
    14641 tggcatccca ctcgggtaat cttaacgcat cagcagccac atcgagcttt ctctcaagat
    14701 cccaaccgcc acaagcttct atctcatttt gcagctcacc ttgttccatt aacaaggtat
    14761 tcatttcatc atcgctcatt ggttctgcaa aacgcatact aatcgcatcg aaacgagaca
    14821 gtttctcttt aatttcagcg acaccttcct caactatttc gcggacagtt ttatttaagt
    14881 caagctcagg ttcttgagca agatatccaa ttttaattcc tggttgaggt cttgcttcac
    14941 cttcatattg tgtatcaaca cccgccataa tccgcaatag agtagatttt ccagagccat
    15001 ttaatcctaa tacgccaatt tttgccccgg gaaaaaaact taatgaaata tcctttaaaa
    15061 taaacctttg gttctcaaca atcttgctca ctctattcat agtgaatata tactgcgcca
    15121 taatcgctcc aaatctctat aatcagcctt ttatcaaaca atactaccag ttcagtataa
    15181 ctttccctga ctggcccgat gccataattt gaaatgcgtg ctgatattca tctactggga
    15241 aattatgagt aatgacagga gaaatattta aaccgctctg taacatagca atcatcttat
    15301 accaggtttc aaacatttct cgaccataaa tacccttaat aaccaatcct ttaaagataa
    15361 cctggttcca atctataggg gtttcttgtg gtgggatacc caataaagcc acatgaccac
    15421 catgattcat cgccttcatc atatcgttta atgccatagg gttcccggac atttccagac
    15481 ccacatcaaa accctcaagc attcccagct cattagccac atcaattaat ttttggtatt
    15541 tgacattaac cgctcggctg acacccattt gcctggccag ttccaatcga tgatcgttaa
    15601 catcggtgat aacaacatgc cgtgcgccaa tatgtcttac gatggctgcg gccattattc
    15661 ctatgggacc tgcaccagta atcaaaacgt cttcgcctac tacatcaaat gccaatgcac
    15721 aatgagcagc atttccaaaa ggatcgagaa tagccgcttg ctcttctgta atgttgtcag
    15781 gtagagcaat gacattggta gcaggaagtg ccagatactc agcaaagcat cctggtcgat
    15841 tcacccccac tcccagagta ttacgacata aatgacgctt gccggcacga caatttctgc
    15901 aaaaaccaca ggtaatatga ccttcaccag aaactctctg tccaactttc aaaccttgga
    15961 cttcttttcc aacctctact atttcaccat aaaattcatg ccctaccgtc ataggaacag
    16021 gaattgtcgc ttgcgcccat tcatcccagg aataaatatg gatatctgta ccacaaattg
    16081 cagtcctttt aatttttatt aaaacatcat ttactccgta ttcaggaaca ggaatatcct
    16141 gcatccaaat tccaggctct ttttttgctt taaccaatga tttcattgtc atacctcaaa
    16201 ttaattaggt aataccacca tttcgcaatt catcctaaaa tatcaggttg tgaaatgtag
    16261 agtaatttag ttttagatta cagagaattc cttaccaacc gtttcaaacg ccttcaaagc
    16321 tttatccaaa tgatgcaatt caagagcagc agacatttgg gtacgaatgc gagccaaacc
    16381 tttgggtacc actggatagg aaaaccccac aacatagaca cccagatcca gcaagcgatt
    16441 agcaattcgt ccggccaaac tggcatctcc taacatgaca ggaataatag gatgttcacc
    16501 cgggattaac tcaaaaccta actgagtcat tccttcacgg aaataatgac tattgcgctt
    16561 taatttttca gcaagggagt tatttgtgga caatttatcg agcaccacac aagaagtatg
    16621 agcaataacg ggtgctaatg tattggaaaa cagataaggc ctggatcgtt ggcgcaacca
    16681 atcaatgaca acttgatttg aggcagtata tcctcctgat gcgcccccta aggccttacc
    16741 aagagtgcca gttacgatat cgattctatc actaactcca aaatgctctg gtgttcctct
    16801 acctgtctta cccatgaaac caacagcatg agaatcatca accatcacca tggcgtcata
    16861 cttgtcggct agttcacaaa ttgctggcaa gttagctaat ataccgtcca ttgaaaaaac
    16921 accatctgtt gcgatcaaac gaaatctcgc attttttgct gcaattaact gttcttccag
    16981 ggccttcata tcattattgg catatcgata acgtgcggcc ttacacagcc tgactccgtc
    17041 aataatactc gcatgattca gggcatcgct gataatggca tcttcttccg tcaataatgt
    17101 ctcaaataat cccgtattgg catcaaagca ggaagaatac aatatagtgt cttcttgact
    17161 taaaaaatgg ctaatctttt gttctagttg cttatgcggg gtttgtgttc cacaaataaa
    17221 acgaacagaa gccatgccat atccgtactt ggctaacgca gcctgtccct ccgctattaa
    17281 ttcaggatca ttggccaaac ccaagtagtt atttgcacaa agattaataa cttctctttc
    17341 gttaaccttc acgtcagctt gctgttggct ggaaataact cgctctgatt tatacaatcc
    17401 gtctgccttg agtgtttcaa gttcattttg taaataatta atgaatcgct caagcactat
    17461 aatctcctgt atttctggat aaatttgtaa ataataccaa attttaaaca ttctcaccat
    17521 gagatattat caagaaaatt tcactttcaa acattaatgc ttagacataa gctgaattct
    17581 tgctaaaatt gctccaaagt tttaacctat ccaatgatta ttaagacggt agagcaaatg
    17641 aatcacagtg cacgcataaa tatgatcaaa caacagttac gaactggtga tgtattaaac
    17701 gaatccatcc ttgacttata cgatgagctc ttaagacatg agtttgttcc tgaacctttt
    17761 tctcattttg cttattcaga catgcaaatt cctttggcat acggtcaaag aatgttaact
    17821 ccattggaag aaggtacaat tttacaatcc cttgatctta aaggtcatga aactgtgttg
    17881 gaagtgggaa caggaaccgg ttttatgact gcattactca gtaagttatg taaaaaagtt
    17941 atcagtattg actattattc tgaatttact gccaatgcaa aacgcaaact cgaagagcat
    18001 aattgtaata atgttgaatt aattaccgga gatgcttgtc gtggatggtt ggaaagtgca
    18061 ccatacgatg ttatggtatt cactggtgcc atggaaaaac tgacagatac ccataaacta
    18121 caaattttac ctggtggcaa gttgttcgca atccttggta aatcccctgt aatgaaagct
    18181 tatctttttc agttggatca taatgcaatc tggacagaat ccatgctttt tgaaacggat
    18241 atacctcctc tggtagatca attgaaaccg aaagaatttg ttttctaggg aaaactcgaa
    18301 tgagaaaatc tctgttttgt tggattttaa ctttaggtgt ttcaacccat gtgtttgcaa
    18361 cagacctaat ggatatctat caacaagctc tggagaacga taccattttt aaagaggcat
    18421 atgataccta tatgtccagc acagaagcca tacctcaagc tcgtgccgct ctttaccctc
    18481 aagtaggcct tggttcacaa gctggtcgca attatcagga tgctgttgct ggagctttca
    18541 gtgccaatca atactatgga agctatttgt ggcaagtcaa tgcttctcaa gcactgttta
    18601 actatcaggc atgggccaag gtagctcagg ctaaagcctc tgttaaagca gcacaagcca
    18661 ctttcaatga tgcagcacag aatttaattc taagaacggc caaagcctat tttgatgtct
    18721 tgtttgctaa agacacttta gactttgccg aagcaaaaaa acgggcgaac aaaagacaat
    18781 atgatcaagc aactcaacgt tttcaagttg gcctggatgc cattacttct gtctatgaag
    18841 caaaagcagc gtatgatcaa tccatagcga cagttatcgc cgcccgcaat aatcagatta
    18901 atcagagtga aaatttacga aaacttacta atcacgtata cgaaacactc gcgccactca
    18961 aagacagcaa aattcctctt gttaagccag agccaaatga cgtgaatcaa tggatagata
    19021 ccggacttaa gcaaaactat aaattatatg ctgcaaaata taatcttgag gtggcaaaag
    19081 acaatgtaaa agcgatctct gcgggcaact ggccagtttt ttcactacag agcaacgcaa
    19141 gccaggtaca taataatgca tccggcaata ccgtttttat tccgtcaaaa caaacccagg
    19201 ctaatattgc aatcgcgatg aattttcctg tttttcaagg cggcctggtc caggctcaaa
    19261 cccgacaagc tcaatatggc tttcagtcta cgagtgaaaa actggaacaa acgtatcgcg
    19321 atgtcattgt aaacagccgc attgctttca ataccatcac tgatgggatc agcaaagtaa
    19381 aggcggacag acaaacggtc atctccgtgc aaaattcttt gcaaagtact gaagctcaat
    19441 ttgaagttgg tactcgaacc atggtagatg tggtcaatgc tcaacaaaga ttgtttgaag
    19501 cacaggaaca attagcccgg gatcaatacg atttgattaa ttccatcctt acgctgaaat
    19561 accttgcagg gacattaaac gttaatgatc tcgaacagat taactcatgg ttagcaacta
    19621 cacgtgtcaa tggattttca cccgtagata acaaaacctc caaataacaa atttaggttt
    19681 ttttatgtct tccaatcctt cttcagttga aaaaaaatta ctccattata caggtaaagc
    19741 catagctgat tttaatatga tccagcgtgg tgacagagtc atggtatgtc tgtctggcgg
    19801 aaaagattca ttcacactat taacaattct taaccagctc aggatcaaat caggaaataa
    19861 atttgaaatt tttgcgttta ccctggatca agcccagcca gggtggaatg atgcctgttt
    19921 gcgtcaatgg ttggctgaaa aatccattcc tcatgaaata ctaacccgag acacatacag
    19981 tatagtgaag gaaaaaattc ctgaaggcaa aacctactgt tctttgtgtt ctcgcttgag
    20041 acgtggaatc atctacagat atgctgaaga aaacggattt aataaaatag cattaggtca
    20101 ccatcgtgat gatcttattc gtacattgat gatgtcaatt ctatataatg gcgatatacg
    20161 ttccatgcca cccaaattat taagtgataa taaaaagcac atagtcatcc ggccactgtg
    20221 ctatgtgcag gaaaaagata ttattacgtt tgcatcagaa caagcctttc caattatccc
    20281 atgcaatctt tgtggttctc aagaaaatct aatgcgcaaa aaagttgcct cactcattga
    20341 tcagcttgcg attgaaaacc ccaaagtccc cagtaacatg cttcatgcgc tgcaaagttt
    20401 gaagccaagt caattgatgg atcaaaattt ttggaatttt aaaaatctgg aagatgggct
    20461 tgaaaccgcg cagtcaat
//
LOCUS       DACTJE010000042        20284 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.042, whole genome shotgun sequence.
ACCESSION   DACTJE010000042 DACTJE010000000
VERSION     DACTJE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20284
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.042"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..541
                     /gene="ceg5"
                     /locus_tag="I9760_001206"
     CDS             <1..541
                     /gene="ceg5"
                     /locus_tag="I9760_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg5"
                     /protein_id="HAT5042982.1"
                     /translation="QEWRDRFIQKMGGISHFASSDKFNANKECPQTYPENPTTLYLST
                     YTAKNGELAVVFPNKEIRQNFLTLFRHASSATWSIYEGQDAVYFNDKNLHTPGYNFGI
                     NAPACVSQSSNAAHADVRYAARILGQALRNPNNTCFFNRLPEELINKIISLSTDNEVL
                     AAEDAISLVQPNLGKPQLK"
     gene            complement(746..1315)
                     /locus_tag="I9760_001207"
     CDS             complement(746..1315)
                     /locus_tag="I9760_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAT5042983.1"
                     /translation="MKNKLLPNLIIITLAATIYANSINVQAKQNDQIQNSNEQLTQDP
                     FDTDPFFQSHDDVLLQIHKMQQAMDQFIKSQFSQMQNTLANDPHHNPFGSAKNIQITE
                     NKDEIIYKIKLPQGSDSKVDITVKNGQLVLSANVTQKITREQDNNKSVSFSQSNYMQS
                     FPLPQSYDPKSMVTKMKDSNLIVTFKKNS"
     gene            complement(1799..4048)
                     /gene="katG"
                     /locus_tag="I9760_001208"
     CDS             complement(1799..4048)
                     /gene="katG"
                     /locus_tag="I9760_001208"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAT5042984.1"
                     /translation="MFKRTIPLFAAFTLAISPSIFPNYAHAQEDKPKTNQYWWPKMLD
                     LSPLRQPNATSNPMGEKFNYAEEFNSLDLNAVIEDLKKLMTTSQDWWPADYGNYGPLF
                     IRMSWHAAGTYRIYDGRGGANGGFQRFAPQNSWPDNANLDKARRLLWPIKQKYGRKIS
                     WADLLVLAGNVAMESMGFKTIGFAGGREDAWEAININWGPEGKWLESKRQDKDGKLEK
                     PLAATVMGLIYVNPEGPNGVPDPLAAAEKIRETFGRMAMNDEETVALIAGGHAFGKTH
                     GAASGKYLGPAPEAAGIEEQGFGWKNSYGSGKGKDTITSGLEGAWTVTPTHWSHNYLQ
                     NLFNFNWVKTKSPGGAIQWVPENSNASSMVPDAFDPSKRHAPVMLTTDLALKFDPVYS
                     KIAKRFLDNPKEFDDAFARAWFKLIHRDMGPRSRYLGSLVPKEAMIWQDPVPPVDYKL
                     VDANDIANLKGKILNSGLTTSELVKTAWASASTFRGTDMRGGANGARIRLAPQKDWPA
                     NDPQELAKVLKTLESIQNNFNNAQADGKKISLADLIVLGGNAAIEQAAKQAGYDIIVP
                     FTPGRTDATQGMTDVKSFEVLEPKADGFRNYFDKSNNMSPPEMLVEKASLLKLSVPEM
                     TVLVGGMRVLNANTGQNQYGVFTDKPGTLNNDFFINLLSMSTEWKKSSETEGIYEGYE
                     RKTGKLKWKATSVDLIFGANSELRAVAEAYATDDAKEKFIQDFINAWVKVMTADRFDI
                     KAANANINS"
     gene            complement(4180..5184)
                     /gene="ravE"
                     /locus_tag="I9760_001209"
     CDS             complement(4180..5184)
                     /gene="ravE"
                     /locus_tag="I9760_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavE"
                     /protein_id="HAT5042985.1"
                     /translation="MENDMPKTKYALPPVVLFESHADRSTSDFLIKQLPDLKKAGYTT
                     ICVDGMEPCASLEENISMMKILIQIQVKKLSELPLEHPEYAQGVEKLRSVVAKLDLFE
                     AMKEQGFKLGGIDLPVSEQLKEKSLNSIRREQTITDNTLKHVKENDGGVVVVLGFGHC
                     IFQQMIKEQDENANQYLWYHVHNPDNETQAYKELVESYTKKGLSTYFPLGVNIFKSSD
                     KELDTDFWNKVSANCYNYDPKALETSTASILKSLVGPEVTAHLRTDGQHHVDALISLE
                     TVEKTHQIKSSDFLRSLSKTLGNIHFEVAKIKTKDQVIIRGINEPEVAEQISKLSKKM
                     "
     gene            complement(5386..6660)
                     /gene="ravF"
                     /locus_tag="I9760_001210"
     CDS             complement(5386..6660)
                     /gene="ravF"
                     /locus_tag="I9760_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavF"
                     /protein_id="HAT5042986.1"
                     /translation="MLLGDFIMSLEIYTWKNGSKGLGFTKPVKSMLLGGNVGHAAVEL
                     TFPADAKGDELAKKYQDVPGLSISKRTEIVPEKQENGSYKPKEQVVYFVYFSWWPGHT
                     NGHHINSHREDLESEWRHEPAPKIKPEIQEHLYGENIPTANKTNVTGLLISDKTITKV
                     KEISHQSIKETTTIENDPAYQQLKAEEAALQTELQQLIDKRKIYEQELARSGLEQRQP
                     NKELCLTDAEIARGYEISKELKRVAEQIKLCQIDFEERYLSVGEPPSSVIRIPTTLDH
                     NAPTFALDAAGILDKMASLAQSTTAYSFYKFNCSTSATQVVKAGISDELKNIMKTDGF
                     NVEKASQTTIATPTSFRHFSQQVQTELILLNTNQALVEQQRAKTENTSEAPTTSTQSF
                     KQKFQDTVKEKSEKAVREQDESEENRIGLTIS"
     gene            7019..7336
                     /locus_tag="I9760_001211"
     CDS             7019..7336
                     /locus_tag="I9760_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HAT5042987.1"
                     /translation="MTMMITTGNSFEGKVIKQYLGIVRGIVVRSPTISQGLMGGLKSI
                     VGGKIGAYSEMCEHAREEAFQLMIEHAQALNANGIIAMRYDTGEIGQAGTEVLCYGTA
                     VII"
     gene            7444..8475
                     /locus_tag="I9760_001212"
     CDS             7444..8475
                     /locus_tag="I9760_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042988.1"
                     /translation="MNIVRIFLSFLFLFFNCLGFAQSRVPQLFLFLGGDKASSFQNTL
                     QNPCIQGVQIIYSWRQLEPQKGVYDFSKIEEDWNFLSRIDKKLFIQIQDRSFSPDVFH
                     VPDYIRLEKAYHGGVVQQYDFPGEGKPMTTGWVARTWDPAVRKRFQLLLKKLASKFDG
                     KIYGINLSETSIDLDPDNLPEDFSSDKYFYSVLDNIGYLKKAFQKSRVIQYVNFFPDE
                     WNNDHHYMSRLFAFARKHHIGLGGPDVVPYKKSQMKNSYPFFHKFKGKIETAIAIQEP
                     DYTYTNPKTGDFYRFDDFYSFSQEYLGAFILFWNTEEPFFSEQLLPRLNRTYFNCKKG
                     NNESQAATA"
     gene            8591..9961
                     /locus_tag="I9760_001213"
     CDS             8591..9961
                     /locus_tag="I9760_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAT5042989.1"
                     /translation="MLVELLSRIQFGFSIGFHILFPTLNLGLAVFLVIMEAMWLKTKN
                     PAYLEICKFWTKIFALTFGMGVVSGIVLAYQIGTNFGPFITQFGNVLGALFAYETLTA
                     FFLEAGFLGVMLFGWKRVPPALHFIATLLVTIGTTVSAFWIMSANSWMQAPSGYQLIE
                     GKYVVDSWWAVVFNPTFIPRFIHMLLASYVTTCFVIMAVSGFYFLRKQSTAIAKKSLS
                     FSLWASLILVPLQIGVGDVVGINVHHYQPLKTAAMEGIWETQKGAPLLLFAIPSQTQQ
                     KNFYEVGIPKLASLINTHEWNGEMIGLSSVGNEEQPRMAPVFFSFRIMVGIGLIMLFT
                     AIAGFILRLRGTLFENSWFHRWCIVMAPLGFIASIAGWLTAELGRQPWVVHGLLKTRD
                     SVSAIGVEEVIISFVLLIVAYGVVFGFYLYYLLRLISHGPGILEEDEAEHHAFQYMTD
                     FPKEKK"
     gene            9961..10950
                     /gene="cydB"
                     /locus_tag="I9760_001214"
     CDS             9961..10950
                     /gene="cydB"
                     /locus_tag="I9760_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAT5042990.1"
                     /translation="MLPFIFATLLGFIVLMYVILDGFDLGIGILFPFTSSESERDKMM
                     NSIAPVWDGNETWLVFGGAMLYGAFPQVYGLLLPILYMPIMLMLIALIFRGVSFEFRF
                     KAKRSKTLWNWSFSISSVAATFLQGVILGAFVQGFSINESSMTINDPDWLTPFSLCTG
                     IALVCGYALLGSTWMIIKSTGRLQRKMVHYARGLLVLVSFFLLFVSIWTPLHSEEVFH
                     RWYSFPNVLLLSPLPLIALMAIYLVWQNLNSGSEYKPFIYSIIIFLCSYIGIAISVYP
                     YLIPHQVTLWEAAAPDSTLVFILIGVAIMLPILLAYTLYAYYLFRGKSSENYH"
     gene            complement(10951..11736)
                     /locus_tag="I9760_001215"
     CDS             complement(10951..11736)
                     /locus_tag="I9760_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HAT5042991.1"
                     /translation="MNKMIILLVIAIIFLHMCLIWLWYRYTNNPSVVDVGWASGLTLA
                     GLIYLNNQEFSLRSLLLVLALLTWGIRLGGYLWWTRIRLKKIDKRYLALSNDWKLAKP
                     LGFFLNFQLQGVLICLVSIPWYFSSSETQTALNLLDGLALIIFLTALILETVADNQLQ
                     DFKKNYPGKVCNQKLWRYCRHPNYFYEWLVWCSFTLFALSAPYGWIAIISPLTLYLIM
                     TKITAPMTEQGSIESRGNLYIEYQKVTPMFFPKWLKNNSTPRK"
     gene            complement(11733..12269)
                     /locus_tag="I9760_001216"
     CDS             complement(11733..12269)
                     /locus_tag="I9760_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HAT5042992.1"
                     /translation="MIINPVHIVHYLSYYLCWIFCIYLASYGYPLLGLIITVLIMGLQ
                     IIVQILNQLPWRGALYFSMGLALLGAITDAIWLYSGMIYFKANFFGSHFPPLWMVALW
                     LSFGFNLVVLYGSWLTYYFLWGLVMFPSIIFAYWLGVQFGAATLIGSEVVFYLTLGLT
                     WSLFLPLSLYLFNYRHRK"
     gene            complement(12259..14046)
                     /locus_tag="I9760_001217"
     CDS             complement(12259..14046)
                     /locus_tag="I9760_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family EDD domain-containing protein"
                     /protein_id="HAT5042993.1"
                     /translation="MPLDKLIGITFHAAIIAGCHRLIRHKQILNAINVFPVADGDTGD
                     NLSSTCMAIIYFSSVKADLRGTLESIADASIIGARGNSGIIFSQFFNLLAKHLPLKEE
                     LYFNDFSKIMVQVGKEIAAVVTNPVQGTMITLIQKWATVCEIKQPDSLLAQAMLELLP
                     LLFMEVEQTKNSLNILQKANVVDAGALGFYYFVEGFTQFLQQSHTTDTANTHSPLSIT
                     EHPQLTCTTTEYRYCTEAVLKSASIDKSKLLHFLKEQGECASVTGNDRITRFHVHTNN
                     PREIFTSLYKDYTIQYPKVDDMQRQHELQFGKKYKIGLVTDSSADLPQDLIDEYQIHQ
                     IPLNIHVDEHHLLDKHCFEANYFYTQLNSFRTYPKTSCMNPNLIEEKLAKIASHYDHV
                     LVLSISSKMSGTYDAFLTATKKYANITVLDTKTNSGAHGLLLHYAGQLIASGNDIDTI
                     TKLLGKAIKNTYIFVMVNQFESMIRSGRVNKISGRIAQWVNIKPIVSIDSAGQGIMVG
                     KCFSASRARNKLINFLQEKKDEAQLILEDYCIIHAGDKNLALHLAEMTTQAFNKPPTY
                     IESVSLAIGLHAGQGCIAIAGRLRECNDY"
     gene            14129..14827
                     /locus_tag="I9760_001218"
     CDS             14129..14827
                     /locus_tag="I9760_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAT5042994.1"
                     /translation="MARYLVIAASSAIGQSVTTLLKNRGDTVFTTARDNHKISPDFQL
                     DATHFDAVLDVFRQTGPLDGVVNCAGSLLLKNAHSTSFDEFQVVINASLTTSFATIRA
                     AGLTMNQGGSVVLISSAAALVGLANHEAIAAAKAGIIGLAQSAAATYASNNLRVNVVA
                     PGMVNSPLTSSLLSNQLVLNASKGMHALGRIGVPEDIAHAIVFLLNPDNSWITGQVIA
                     VDGGLSRVRPKMKI"
     gene            14844..16382
                     /locus_tag="I9760_001219"
     CDS             14844..16382
                     /locus_tag="I9760_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HAT5042995.1"
                     /translation="MTTLCFILGDQLNETIASLAAINKQKDIVFLCEVAEETSYVPHH
                     PKKIAFLFSSMRHFAQQLKKKGYRVRYTTFDDPRNQGSFAKELLRAIEEEQVSAISLT
                     EPGEWRVLKIFHGLKSQLTIPVTLHEDNRFLCKINEFRNWAKEKKQLRMEFFYRMMRQ
                     KYHLLVDNNGKPVGGTWNYDAQNRKNANHISSFPKRLEHTRDVITDEVLGLVSARFAK
                     HFGQCYPFNLAVTREQALEEANYFMEHFLCNFGAYQDAMLKDEISLYHSKLSVYLNAG
                     LLLPLELCQMAEQAYFSKQMPLNSVEGFIRQILGWREYVRGIYWLFMPDYGEMNYFNA
                     CRPLPSFFWGEETGMFCIKEVVRQTSTEAYSHHIQRLMITGNFALLAGLDPKQVCEWY
                     LAVYADAYEWVELPNTLGMALYADGGLMASKPYAASGKYIHRMSNFCQSCYYNPNDLL
                     GEKACPFNALYWNFLHQNQEKLKDNPRLRYAYMNWQKMDFDKQKKTIHKAQRILADLD
                     SANL"
     gene            complement(16387..16800)
                     /locus_tag="I9760_001220"
     CDS             complement(16387..16800)
                     /locus_tag="I9760_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HAT5042996.1"
                     /translation="MNGWTLKVFAIAFFIFIVTDMIWLGFIARNLYFDQYQQWLRLSE
                     GQLKPIWWSALLVYLLFALSIIVFVIPLAKTSLPYAALYGALLGAIIYGIYDFTCLAI
                     FKDFPVGMAFLDWLWGIVLCSWCSVATIYVASYIK"
     gene            17164..20049
                     /locus_tag="I9760_001221"
     CDS             17164..20049
                     /locus_tag="I9760_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HAT5042997.1"
                     /translation="MKLLRFHELKSLPSMDEKALELLIKVLGNKGIRKLIKSADGKPI
                     SREIMIHEFGIDCQILFITTEASLKPIIVPTENKISGGGKSYCEQFKVYALDDGKTYF
                     LKSVKINAESLTEFTNETDTLSKLGRLVGTFFNEQTQVHYILTTFIKGIDLSRYKNAL
                     PLNINLKHFWEVLGIMISVCHQVKQFHELGLIHRDLKPGNIMLDADMQCHLVDFGSSS
                     SDKEPKPVSWGTASYLAPELNAQEDFIAFSQASDLFALAYSLDELFNPFRQVKFAKVD
                     IGIKNKHLVLLHAEIEACITGLMSNETSVRTLYFSRILQLQRVPESFKSRPEAFTYMI
                     MLLTQWKSCYEAPEMNKELDEIIAEIKVAYENHEQDVVKIITLLEQLSKADGLLNSHK
                     ALLSVLIKSLANVQQQELGQDDILPRRFESDVVSRLIKTPTAKMMAAIKQVSDAIVKI
                     LEQYEHSPAESFEHAVLQDFLEQMVQSDILFGAPKEKIEISDIIKILKANRPEDFVQI
                     QHISFKFAQVALRDLALHDLPKHEPKGAFLEILKKYQAKYAPEETSLFELYHHMAGVR
                     DHNPSKVKKGEGYRFRAFICDELFYGDEIFTTGDNRGREGKPNQRLQTNQVGLMKFEH
                     SAHTKGLLTLNGQSWYADCKTQLPNYDSIHYLSALKTDCPYITGPSGMTSLFMNMMFL
                     LLNPKDQDVILSYSLGVMTYVVGAGYHSIKEILIPMVKCVGIVPDYPQHKGTECLTAP
                     PLYNHYFKAIEEFDKEFAEVHEKIWQEYLAYFRMTYMPVCMRSHCLPHQVPSMEDISS
                     EVKEMLEIVSKSVKNCLEEHKVKRTGDMGLFLSTPCGQTKALEVLSHVCQQQVGLTAI
                     FRQIQAYFKGTFETEEGIIINREMQLKFIAHFFDVLKESPTLLSQFNLTLRIENTIGV
                     HQCELGSSKQKELFLEIKKTSIAANEEQMVANHEPRKENRNVIILPY"
     gene            20068..>20284
                     /locus_tag="I9760_001222"
     CDS             20068..>20284
                     /locus_tag="I9760_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5042998.1"
                     /translation="MLSKEKINPFLKNTKRQGSDNLAAMADPTSLKNTGDYRCFNSKP
                     EAYTYFIFLLNQWQTNYIDDKREAIAKA"
BASE COUNT         5839 a         3869 c         4169 g         6407 t
ORIGIN      
        1 ccaagaatgg agagaccgat ttattcaaaa aatggggggc atatctcatt ttgcatcttc
       61 tgataaattc aatgccaata aagaatgtcc gcaaacttat cctgaaaatc ctactacgct
      121 gtatctcagt acctatactg caaaaaatgg ggagttagcc gtagtttttc ctaataagga
      181 aatacgtcaa aactttttaa ctttatttag acacgcctct tcagcaacat ggtcaattta
      241 tgaagggcag gatgcggtat attttaatga taaaaatttg catacccccg gatataactt
      301 tggtattaat gcacctgcgt gtgtttcaca atcatctaat gctgcccatg cggatgttcg
      361 ttatgctgca aggattttgg gccaagccct tagaaatcca aataacactt gcttcttcaa
      421 caggcttcca gaagaattaa ttaataaaat cataagctta agtacggaca atgaagtgct
      481 tgcggcagaa gatgcaataa gtttggttca acctaactta ggtaagcctc agttaaaata
      541 atcagctatt tttaaaaatg gtgccggggc ttgaatttta tgtgcaaaga cagggaatct
      601 ttgcaataga aaattcaaac ccaggctctt gatttccttt aaccagcgct gcagaaaatc
      661 aaaatttcaa gccggcatca aactgtataa tcaatccttt cccatggttg ttttagcagg
      721 ttatttaatt aagcagataa tgcggttaag aatttttttt aaaagtaaca attaaatttg
      781 agtctttcat ttttgtgacc atggacttcg gatcatagct ctggggcaat ggaaatgatt
      841 gcatgtaatt gctttgggag aaactgacac ttttgttatt atcttgttca cgggtgattt
      901 tctgagtgac gttagcgcta agaactaact gcccattttt aacggtaata tccactttac
      961 tatcagaacc ttgcggtagt tttatcttat aaataatctc atctttattc tctgtaattt
     1021 gaatattttt tgcactgcca aagggattgt gatgaggatc gtttgcaagg gtattttgca
     1081 tttgtgaaaa ttgacttttg ataaattgat ccatagcttg ttgcattttg tgtatttgca
     1141 gcagaacatc atcatgtgat tgaaaaaaag gatcagtatc aaatggatcc tgggttaatt
     1201 gttcgttact attttgtatt tgatcatttt gtttcgcctg aacgtttatg gagttagcat
     1261 atattgtagc ggcaagagtg atgatgatta aattgggaag tagtttattt ttcattaaaa
     1321 atctcctcac aagatttgtt tataaaaaat tctatctcta tttgatatgg atgtttttac
     1381 ttcaatccac tcattagccc tcttgcataa cctgccctct ttcattttat tgctgaatgc
     1441 aaactccgct cctcaaaacg ttagtgcctt ggcctgaaac caattaatca gcttaggcaa
     1501 gaggtctatt ttaaagcggt tatctcttat tggtgaacat gaaagtcgca tgcaaaatat
     1561 tgggttaaac cagatttttt caagtacaaa gtggcgaata tttgaaatgg aataaagccg
     1621 tataacacgt aattcaagag agggcatagg ccaagctgga aaaccataga ggtttggcta
     1681 aattagccgg gctttgttaa accagtataa gtcagagcct tatccggtga tttgggattt
     1741 agggtaacaa acgatgattg agtcccagtt tgtcacctta aatctgaaag aatgaggatt
     1801 aactgttaat gttggcattt gccgctttga tgtcgaagcg atcggctgtc attaccttaa
     1861 cccaggcatt aataaaatct tgtataaatt tttcttttgc gtcatcggtt gcataggctt
     1921 ctgcaactgc tcttaactca gaattcgcgc cgaagatgag atcaaccgag gtcgctttcc
     1981 atttcagctt tccagttttg cgctcataac cttcataaat cccctctgtc tcacttgatt
     2041 tcttccactc tgttgacatg cttagcagat tgatgaaaaa gtcattattt agagttccag
     2101 gtttgtcagt gaatacacca tattgatttt gtccagtatt cgcgttaagt acacgcatcc
     2161 caccaactaa aaccgtcatt tccggaacac ttaatttcaa cagactcgct ttttccacca
     2221 acatttctgg tggcgacata ttgttgcttt tatcaaagta atttctaaac ccgtctgctt
     2281 taggttcaag aacctcaaaa gacttcacat cagtcattcc ttgtgtggca tctgtgcgtc
     2341 ctggcgtgaa gggaacaatg atgtcgtagc cagcttgctt ggccgcttgt tcgattgcgg
     2401 cattaccacc taaaactatc aaatccgcaa gagatatctt tttcccatcg gcttgagcat
     2461 tattgaagtt attctgaatg ctctccaatg tttttaatac ttttgccagc tcctgaggat
     2521 cattagccgg ccagtctttt tggggagcca ggcgaattcg agcaccattt gcccctccac
     2581 gcatatcagt gccacggaag gtggatgccg aggcccaagc agttttgacc agctcggatg
     2641 tggtcaatcc cgagttgaga attttaccct tgaggttagc aatatcattg gcatcaacta
     2701 atttgtaatc aactggcgga actggatctt gccaaatcat ggcttcttta ggcactaaag
     2761 agccgaggta acgtgaacgt ggccccatgt ctcggtgaat cagcttaaac caggcacgag
     2821 caaatgcgtc atcgaactct ttaggattat caagaaaacg cttggcaatt ttactgtaaa
     2881 caggatcaaa ttttagtgcc aagtctgtgg tcagcatgac cggcgcatgc cttttactgg
     2941 ggtcaaatgc atcgggaacc attgacgagg cattcgaatt ctcaggtacc cactggattg
     3001 ctccaccggg gctcttggtt ttaacccaat tgaaattgaa caggttttgt aagtagttat
     3061 ggctccagtg ggtaggtgtt acggtccagg caccttctaa accactggta atcgtgtctt
     3121 tccctttgcc gctgccgtaa ctgtttttcc aaccaaagcc ttgttcttct atgcctgctg
     3181 cttctggcgc tggccccaga tatttgccgg acgcggctcc atgtgttttt ccgaatgcgt
     3241 gcccgcccgc aattaaagca actgtttctt cgtcattcat ggccatacgc ccgaaagtct
     3301 cgcgaatttt ttcggccgca gcaagaggat ctggaacgcc gtttggtcct tccggattca
     3361 catagattaa gcccattacg gtcgcagcaa gcggtttttc aagttttcca tctttatcct
     3421 ggcgcttact ttccagccat ttgccttcag ggccccaatt gatattgatg gcctcccatg
     3481 cgtcttcacg ccctccagca aatccaatgg tcttgaagcc catagattcc atcgcaacat
     3541 ttccagcaag taccaacaaa tcagcccatg aaatcttgcg cccatatttc tgcttaattg
     3601 gccacagtaa acgtctggct ttgtccagat tggcattatc cggccagctg ttttgcgggg
     3661 cgaaacgctg gaagccaccg tttgctccac cacggccatc atagatgcgg taagttcctg
     3721 ccgcatgcca tgacatgcgt atgaataatg gaccatagtt accataatca gcaggccacc
     3781 agtcttgcga ggtagtcatt aattttttga gatcttctat cacggcattc aaatctaagc
     3841 tattgaattc ttcggcatag ttgaattttt cacccatggg attcgaagtg gcattgggtt
     3901 gccgcagcgg gctaagatcg agcattttag gccaccagta ttgattggtt ttcggcttgt
     3961 cttcttgcgc atgggcataa tttgggaaaa tggaaggcga gatggccaga gtgaatgctg
     4021 caaacagagg tattgttcgt ttgaacatag aaatgactcc cttttctata cagaaaaggc
     4081 aaagtatact ccatgttact tttaagcagc aacaatactt taagcagtta atttaattaa
     4141 ggtaatctaa aaatatattt taatttcaga atattaggtt tacatttttt ttgatagctt
     4201 gcttatctgc tctgctacct ccggttcatt aatgccacgg ataattacct gatcctttgt
     4261 cttgattttg gctacttcaa agtgaatgtt acctaaggtt ttgcttaaac ttctcaagaa
     4321 atccgaagat tttatttgat gtgttttttc aactgtttca agagaaatta gtgcgtccac
     4381 atgatgctgc ccatcggtgc gcaagtgcgc tgtcacttcc ggaccgacta aggatttcaa
     4441 aatggaagca gtcgatgttt caagagcttt tggatcataa ttatagcaat tggcgcttac
     4501 cttattccaa aaatcggtat ccaattcttt atctgagctt ttaaaaatat taactcccag
     4561 tgggaaataa gtagagagtc ctttttttgt gtaggattcg accaattctt tgtaggcctg
     4621 tgtttcatta tccgggttat ggacatggta ccagaggtat tgattggcat tttcatcttg
     4681 ctctttaatc atttgctgga atatgcaatg gccaaatccc aggacaacga ctactcctcc
     4741 atcattttct ttcacatgct ttaaggtatt atcggtaatt gtttgttctc ttcttatgga
     4801 attgagactc ttctctttaa gctgttctga aaccggcaag tcaattcctc caagtttgaa
     4861 tccttgctcc ttcattgctt caaaaaggtc taatttagca acaacgcttc tcaatttttc
     4921 aacaccctgc gcatattcag gatgttcaag gggtagctca gataatttct tgacttgtat
     4981 ctggatgagt attttcatca tagatatatt ttcctccagg gatgcgcatg gttccattcc
     5041 atcaacacaa atggttgtat atccggcttt cttcaaatct ggaagttgtt tgataagaaa
     5101 atcgctcgtt gacctatccg catggctctc aaataacaca actggaggaa gggcgtattt
     5161 ggttttaggc atatcattct ccatttgagg tatatcttta aagtatagtt gattaaattt
     5221 aatcgctcat ttaatgagca acatgttgga gtgtggctat ctaaggaata atttttcaga
     5281 ttattttact tgccagtatg tgttgtagtt atcggttaag tttatcttat tcattattgt
     5341 tatgctggaa ctcaaccgat taattctttt ggaaagatgg aaatattaac ttatggttaa
     5401 acctatcctg ttttcttcag actcatcttg ttctctaact gctttttccg atttttcttt
     5461 cactgtatct tgaaattttt gtttgaagct ctgtgtgctt gtggttggtg cttcagatgt
     5521 attctctgtt tttgctcttt gctgttccac aagtgcttga ttcgtattaa gaagaatcaa
     5581 ttccgtttga acttgttgac tgaaatgacg aaacgatgtg ggtgtagcaa tggtcgtttg
     5641 agatgctttt tctacattaa acccatcggt tttcatgata ttttttagct cgtcactgat
     5701 accagctttc accacttgcg ttgctgatgt ggaacaatta aatttataga agctataggc
     5761 ggttgtgctt tgtgccaaac tagccatttt gtcaagaatt cctgctgcat caagagcaaa
     5821 agttggagca ttgtggtcaa gtgttgtggg tattctaatc acggatgaag gaggctctcc
     5881 aacactgaga taacgctctt caaagtctat ttggcataac ttgatttgct ccgccactct
     5941 ttttagttct ttgctaatct cataaccacg agcgatttcg gcatcagtta aacagagttc
     6001 tttattaggt tgtctctgtt caaggccaga tctggccaat tcttgttcat aaattttgcg
     6061 tttatcaatt aattgctgta attcagtttg taaagctgct tcctctgctt ttaattgctg
     6121 ataggctggg tcattttcaa ttgtggtagt ttctttaata ctttgatggg aaatctcttt
     6181 tactttggta attgttttat cactaattaa tagtcccgta acattggttt tattagcggt
     6241 tggaatattt tctccatata aatgctcctg tatttccggt ttaattttgg gagctggttc
     6301 atggcgccat tctgattcca aatcttctct gtgactgtta atgtgatggc cattggtatg
     6361 gcctggccac cagctaaaat acacaaagta aacaacttgt tctttcggtt tataacttcc
     6421 attttcttgc ttttctggaa caatttcagt acgtttactg attgaaaggc caggtacatc
     6481 ctgatatttt ttcgctaact catccccttt cgcatcagcg ggaaaagtta attcaacggc
     6541 agcatgacca acattgccac ctaatagcat ggacttaact ggtttggtaa atcccaaccc
     6601 tttggaacca tttttccagg tatagatttc aagactcata ataaaatctc caagcaacaa
     6661 tatgatttta ttatgctcta actgattaaa atttaaatta aaagagactt atgtgtgcag
     6721 ggaatttcag cataaaccat tcctgacagg aatgacggtg aatctttaaa gattcatcaa
     6781 gacttatgaa aaacgtcaat gggagcgaaa acctgatctg ataagaggga atatttctca
     6841 gtataaacaa aatggattaa ctattcttgc caatgctaat ctgtaatccg gcaaatgatt
     6901 tttatttata aacaaggagg tctttattta gaaagtttta ataatttttt caataagctt
     6961 ctattttcaa gattttaaac taatatggaa agataaaagg gatatttagt gagcgattat
     7021 gacaatgatg attacaacag gaaactcgtt tgaagggaaa gtgattaaac aatatttggg
     7081 tattgtgaga ggtatagtag tgcgctctcc taccatcagc cagggattga tgggaggctt
     7141 gaaatcgatc gtcggaggaa aaattggtgc gtactcggaa atgtgtgagc atgccagaga
     7201 agaagcattt cagctcatga tagaacatgc ccaggcactc aatgccaatg gcattattgc
     7261 catgcgttac gatacgggtg aaattgggca ggctggcaca gaggttttat gttatggaac
     7321 ggcagtgatt atctgatgcg ttatttttca agtgcaagtc atagagtatt tcccggctaa
     7381 gttaggaggg tatagaggaa atatgatatt attcctggct tgataagatg aacaagaagc
     7441 caaatgaaca ttgtaagaat atttctttca tttctttttt tattttttaa ttgtcttggt
     7501 ttcgcacaat cccgggtacc acaattattt ctatttttag ggggagacaa ggcaagttct
     7561 tttcagaata ctctgcaaaa tccttgtatc cagggagtac aaatcattta ttcctggagg
     7621 cagttggagc cgcaaaaagg agtttacgat ttttcgaaaa tcgaggaaga ttggaatttc
     7681 ctaagtagga tcgataaaaa gctgtttatt caaatccaag atcgttcatt tagcccagat
     7741 gtatttcatg tacctgacta cattcgcctg gaaaaagcat atcacggtgg tgtggtacag
     7801 cagtatgact tcccgggaga aggaaaacca atgactacag gatgggtggc tcggacctgg
     7861 gatcctgctg taagaaaacg atttcagtta ttattaaaaa aactggcatc aaaatttgat
     7921 ggaaaaattt atggcattaa tctttcagaa acttcaatag atcttgaccc tgataatctc
     7981 cctgaagatt ttagctcaga caagtatttc tattcggtcc ttgataatat tggttattta
     8041 aaaaaggctt ttcaaaaaag cagagtcatt caatatgtta attttttccc ggatgaatgg
     8101 aataatgacc atcactacat gagtagacta tttgcatttg ccagaaagca ccatatcggt
     8161 ttaggtggcc cggatgtggt gccttataaa aaatcgcaaa tgaaaaacag ttatcctttt
     8221 tttcacaagt tcaaaggaaa gatagaaacg gccatagcga ttcaagaacc agattatact
     8281 tataccaatc caaagacagg cgatttctat cgatttgatg acttctattc tttttcacaa
     8341 gagtatttag gcgctttcat tttattttgg aatacggaag aacctttttt ctctgaacag
     8401 cttttaccta gattgaaccg tacttatttc aattgcaaga aaggcaataa tgaatcgcag
     8461 gctgccacgg cttaattaaa agggtagaaa tcgtattgtt tttatttgaa gcgaattcct
     8521 gatttaccaa gatatgtata ggcttatatg ctaaaataaa tctactgagc ccatctagtt
     8581 gaggtgacta atgcttgtag agctactatc acggattcaa tttggtttta gcattggctt
     8641 tcatatctta tttcccactt taaatcttgg actggcagtt ttccttgtaa tcatggaagc
     8701 catgtggtta aaaaccaaaa atcctgctta tctcgaaata tgcaagtttt ggactaagat
     8761 ttttgctttg acctttggca tgggggttgt ctctggcatt gttttggctt atcaaattgg
     8821 taccaatttt ggaccattca tcactcagtt tggaaacgtc ctgggggctt tgtttgctta
     8881 tgaaacgttg accgcttttt tcctggaagc tggtttttta ggagtcatgc tttttggttg
     8941 gaaaagagtt cctccagcac ttcattttat cgccaccctt ttggttacta taggaacaac
     9001 cgtatctgct ttttggatca tgtcagccaa ttcatggatg caggccccca gcgggtatca
     9061 actcattgaa gggaaatatg ttgtcgatag ctggtgggcg gttgttttta atcctacctt
     9121 cattcccaga tttattcata tgctgttagc ttcttatgta acgacttgtt ttgtcattat
     9181 ggcggtatcc ggattttact ttttaaggaa acaatctact gcaattgcta aaaaaagttt
     9241 atctttcagt ttatgggcat cgttgatttt agttcctttg caaataggag tgggtgatgt
     9301 cgtgggtatt aatgtgcatc attaccaacc actaaaaaca gctgctatgg aaggcatatg
     9361 ggagactcaa aaaggagcgc cgcttttgtt gtttgctatc ccgtcacaga cacagcaaaa
     9421 aaatttttat gaagtgggaa taccaaaact ggcaagcttg atcaatacgc atgagtggaa
     9481 cggtgaaatg atcggtttga gttctgttgg caatgaagag cagccgcgca tggctcctgt
     9541 gtttttttcc tttcgtatta tggtgggtat tgggctcatt atgttattta cggctatcgc
     9601 tggttttatc ttgcgcttaa gagggactct ttttgaaaat tcatggtttc atcggtggtg
     9661 tattgtaatg gctccactcg gttttattgc cagtatcgct ggatggttaa ccgctgaact
     9721 tgggcgacaa ccctgggttg tccatggtct tttaaagacg agagactctg tttctgcaat
     9781 aggggttgaa gaagttatta tttcttttgt cttgctcatt gttgcctatg gagtggtctt
     9841 tggattttat ctctattatc tgctgaggct catctctcat gggcctggaa ttcttgaaga
     9901 ggatgaagcg gaacatcatg cttttcaata tatgaccgat ttccccaagg agaaaaaata
     9961 atgttaccgt ttattttcgc tacacttctt gggtttatcg ttttgatgta tgtcatttta
    10021 gatggttttg atttgggaat tggtatttta tttcctttca cctcaagtga gagcgaacgc
    10081 gataaaatga tgaactcgat tgcacctgtt tgggatggca atgaaacctg gctggttttt
    10141 ggaggagcca tgctttatgg tgcatttcca caagtttacg gtttgctttt gcccatcctt
    10201 tacatgccta tcatgcttat gctgattgca ttgatatttc gtggcgtgag ttttgagttt
    10261 cgctttaagg ccaagaggtc aaaaactttg tggaattggt cattttcaat cagttcggtt
    10321 gcggccactt ttttgcaagg agttattctt ggcgcctttg tacaggggtt ttctattaat
    10381 gaatcttcca tgactatcaa tgatccggac tggcttactc cttttagcct ttgtaccggt
    10441 atcgctttgg tgtgtgggta tgcgcttttg ggatctacct ggatgattat caaaagcacg
    10501 ggaagactgc agcgtaaaat ggttcattat gccagaggct tgttagtctt ggtcagcttt
    10561 tttctgcttt tcgttagtat ttggacgcct ttgcatagtg aggaagtgtt tcatcgttgg
    10621 tatagttttc caaatgtgct actactgagc cccttgccct tgatagcctt aatggctatt
    10681 tacctcgtat ggcagaactt aaattccggc agtgaataca aaccgtttat ttacagtatc
    10741 attatatttt tatgctccta tataggaatt gcaatcagcg tctaccctta cctgattcct
    10801 catcaagtga ctctctggga ggcggcagca cctgattcga cacttgtttt tatcttgata
    10861 ggtgtcgcta ttatgctgcc aatccttctt gcctataccc tttatgctta ttaccttttt
    10921 cggggtaaat catcagaaaa ttatcactag ttattttctg ggcgtcgaat tatttttaag
    10981 ccatttaggg aaaaacatcg gggtcacttt ttgatactcg atgtaaagat tgcctctgga
    11041 ttcaatcgaa ccttgttcag tcatgggagc tgtgattttt gtcatgatta aataaagggt
    11101 caatggagaa ataatggcta tccatccata aggagcggaa agggcaaata aggtaaagga
    11161 gcaccatacc agccattcat aaaaataatt tggatgcctg caataacgcc aaagtttttg
    11221 attgcagact tttccaggat agtttttctt aaaatcttgc aattggttgt cggcaactgt
    11281 ttccaaaatt aaggccgtta gaaatataat caatgcgagc ccatccaata gatttaatgc
    11341 tgtttgagtt tctgaggaag aaaaatacca tggaatggaa acaaggcaaa tcaatacgcc
    11401 ttgcaactga aaatttaaaa aaaatcccaa aggtttggca agtttccaat cgttgcttaa
    11461 agcaagatag cgcttgtcta tttttttaag gcgtattcgt gtccaccata aataccctcc
    11521 aagcctgatt ccccaggtaa gcaatgccaa gacgagcagt aaagagcgca atgaaaattc
    11581 ttgattattt aaataaataa gtccggcgag tgttaaacca gaagcccatc ccacatcaac
    11641 cacggaagga ttattggtgt aacggtacca tagccaaatc aagcacatat gtaaaaagat
    11701 gatggcgatc acaagtaaaa ttatcatctt attcatttcc tatgccggta attaaatagg
    11761 tataaactca aaggcaagaa tagagaccag gttaacccta aagtaaggta aaatacaact
    11821 tcactgccga taagggttgc ggctccaaat tgtactccta gccaataggc aaagataatg
    11881 gagggaaaca tgactaaccc ccaaaggaaa tagtaagtta accaggaacc atacaaaaca
    11941 acgagattaa atccgaaact tagccagaga gcgaccatcc aaagcggagg aaaatgagaa
    12001 ccaaagaaat tggccttaaa atagatcata ccgctataaa gccagatggc atcagtgatc
    12061 gcgcctaaca aggctagtcc catggaaaaa tagagggcac cacgccaggg gagttgatta
    12121 agtatctgta caataatctg taggcccatg attaatacgg taatgattaa tcccagcaaa
    12181 ggatagccat aagaggctaa gtagatacaa aatatccagc acagatagta cgacagataa
    12241 tgaactatat gcacaggatt aataatcatt acactctctc aggcggcctg cgatagcaat
    12301 gcatccttga ccggcatgta aaccaatggc aagggataca gactctatat acgtgggtgg
    12361 tttattgaat gcttgggttg tcatctcagc gagatgcaaa gccaggtttt tgtcacccgc
    12421 atgaataatg cagtaatcct ctaaaatgag ctgagcctcg tctttctttt cctgaaggaa
    12481 gttaattaat ttattacgtg ctcttgaggc tgaaaaacac ttgcctacca taatgccttg
    12541 gcctgctgaa tcaatggaga ctatgggttt tatattaacc cactgggcga ttcgcccgga
    12601 tattttatta acccgaccag aacgaatcat cgattcgaat tggttcacca taacaaaaat
    12661 ataggtgttt tttatcgctt tgccgagtaa tttggttatg gtatcgatgt cattccctga
    12721 ggcaattagt tgacccgcat aatgcaaaag caatccatgc gccccagaat tcgttttggt
    12781 atccagtacg gtgatattgg cgtatttttt ggttgcagta aggaaggcgt cataagtgcc
    12841 actcattttg ctggatatcg atagaacaag aacatgatca tagtgtgagg caatttttgc
    12901 cagtttttcc tctataagat ttggattcat acaagaggtt ttaggataag ttctgaacga
    12961 gttaagttgt gtataaaaat aattggcctc gaagcaatgt ttatccagta aatggtgctc
    13021 gtcgacatga atatttaaag gaatttgatg gatttgatat tcatcgatta aatcctgagg
    13081 caaatcggcg cttgaatcgg tgaccaaccc tatcttatat tttttcccaa actgcagttc
    13141 atgttgcctt tgcatgtcat ctactttagg gtattgaata gtgtaatcct tatataaact
    13201 cgtgaaaatt tcacgaggat tattggtatg aacatgaaat ctggtgatgc gatcattgcc
    13261 cgtaacagag gcgcactctc cttgttcttt taaaaaatgt aataatttac ttttatcaat
    13321 ggaggcggat ttgagtactg cttctgtaca gtatcgatat tctgtcgtgg tacatgtcaa
    13381 ctgggggtgc tctgtgatag ataacggtga gtgggtattt gctgtatctg tcgtgtgtga
    13441 ttgctgtaaa aactgtgtaa acccttctac gaaataataa aaccctaggg caccagcatc
    13501 cacaacattg gctttttgca aaatatttaa agaattttta gtttgttcta cttccatgaa
    13561 gagtaaaggt agcaattcca acatggcctg agctagcaag gaatcgggtt gttttatctc
    13621 acacactgta gcccactttt gaatgagtgt tatcatagtg ccctgtacgg gattagttac
    13681 gactgcggca atttctttac caacttgaac catgattttg gaaaagtcat taaaatagag
    13741 ctcttctttg aggggtaaat gcttggctaa taagttaaag aattgagaaa aaataatccc
    13801 ggaatttcca cgtgcaccga taatgctcgc gtcagcaata gactctagag tgcctcttag
    13861 gtcagctttt actgatgaaa aatagatgat tgccatgcat gtggaggata aattatcccc
    13921 cgtgtctcca tcggctaccg gaaaaacatt aatggcgttg agtatttgtt tatggcgaat
    13981 cagcctatga cagccggcta ttattgcagc atgaaatgtt ataccaatta acttatctaa
    14041 tggcatggga tgtccgtata atgaatattg aagtttccaa gctaatccat catagtaggt
    14101 atttttatac taatgcaaga agaatgatat ggctcgctac ctggtaattg ctgcaagcag
    14161 tgctatcgga caatccgtga caaccctttt aaaaaatcgt ggtgataccg tttttaccac
    14221 agcacgagat aatcataaga taagccctga ttttcaactg gatgccactc attttgatgc
    14281 ggttttagat gtttttcgcc aaacaggacc ccttgatggc gtagtcaatt gtgcgggttc
    14341 actacttctg aaaaatgctc acagcaccag ttttgatgaa tttcaagtcg taatcaatgc
    14401 gtcactcact acttcttttg ctaccattcg agctgctggc ttaactatga atcaaggagg
    14461 ctctgttgta ctgatatcat cagccgccgc tttggtaggt ttggccaatc atgaagccat
    14521 tgcggcggcc aaagcaggta tcattggttt ggctcaatct gctgcagcca cttatgcttc
    14581 aaacaattta agagtgaatg tcgtggctcc tggaatggtc aatagcccgc tgacctcatc
    14641 gttattaagc aaccagttag ttttgaatgc atcaaaggga atgcatgctt tagggcgaat
    14701 tggtgttccg gaagatatcg cccacgcaat tgtgtttttg cttaatccgg acaacagctg
    14761 gatcacaggg caagtgattg ctgttgatgg cgggttaagc cgagttcggc ccaagatgaa
    14821 aatttaatga aactataatt cttatgacga cactgtgttt tatattaggc gatcaactca
    14881 atgaaacaat cgcctcactt gcggcaatta ataaacagaa ggacattgta tttttatgtg
    14941 aagtggcaga agaaacttct tatgtgcctc accacccgaa aaaaatcgcg ttcttatttt
    15001 cgtcaatgcg tcattttgcg caacaattga aaaagaaagg ttatcgagtc cgctatacga
    15061 cattcgatga tcctcgcaat caaggcagtt ttgccaaaga attgcttcgt gccattgaag
    15121 aggagcaggt gtcagcaata agccttactg aacctggaga gtggcgtgtt ctgaaaatat
    15181 ttcatggttt aaaaagccaa ttgaccattc ctgtcactct acatgaagac aatcggtttt
    15241 tatgcaaaat taatgagttc agaaattggg ctaaggaaaa aaaacagtta cggatggagt
    15301 ttttttaccg gatgatgcgc caaaaatatc accttctggt cgataataat gggaaaccgg
    15361 ttggcggcac atggaattat gatgctcaaa atcgtaaaaa tgccaatcac atcagttcat
    15421 tccccaagcg tttagagcat accagggatg tgataactga tgaggttttg ggcttggttt
    15481 cagcgcgttt tgccaagcac tttggtcagt gctatccctt taatttggca gtgacccgcg
    15541 agcaagcatt agaagaagcc aattatttta tggaacattt tctttgcaat ttcggtgcgt
    15601 atcaggatgc catgctcaaa gatgaaatca gcttatatca ttcaaaactg tcggtttacc
    15661 taaatgccgg gcttttgttg cctctggaat tgtgtcaaat ggcagagcaa gcctatttct
    15721 caaagcaaat gcctctcaac tcggtagaag ggttcatacg ccaaatttta ggatggcgag
    15781 aatatgtgag gggtatttat tggcttttta tgccggatta cggcgagatg aactatttta
    15841 atgcatgcag gccgttgcct tccttttttt ggggagagga aacgggaatg ttttgtatta
    15901 aggaagtggt acgtcagacc tcgactgaag cctattccca ccatattcag cggttgatga
    15961 taacaggtaa ttttgccttg cttgccggcc tggatcccaa acaagtctgt gagtggtatc
    16021 ttgctgttta tgctgatgcg tatgaatggg tggagttacc caatacattg ggtatggctc
    16081 tttatgcgga tggcgggctg atggccagta aaccgtatgc cgccagtggg aaatacattc
    16141 accgcatgag taatttttgc caatcctgct attataatcc caatgatctt ttgggtgaaa
    16201 aggcatgtcc ttttaatgcg ctctattgga attttcttca ccaaaatcaa gaaaaattaa
    16261 aagataaccc tcgccttcgt tatgcctata tgaattggca aaaaatggat tttgataaac
    16321 aaaagaagac tatccataaa gcacagcgaa tattggcaga cttggattct gccaatctct
    16381 gatgtcttat ttgatataac ttgccacata gattgttgct accgaacacc aagagcacag
    16441 tactataccc catagccaat caaggaatgc cataccgaca ggaaaatcct taaaaatagc
    16501 gaggcaggta aaatcataaa ttccataaat aatagcacca agaagcgcac catataatgc
    16561 ggcataaggt agcgatgttt tagccagcgg tataacaaag acaataatac tcaaggcaaa
    16621 aagcagataa actaataaag cagaccacca tattggcttt aattgcccct ctgaaagacg
    16681 cagccactgt tggtattggt caaaataaag atttctggcg atgaacccaa gccatatcat
    16741 atctgtcaca ataaagataa aaaaggcaat cgcaaaaacc ttaagcgtcc atccattcat
    16801 tttattccct taattatgat ttttatcctt ctggttttgt attttacatt caatcggatt
    16861 caggtaatag agcttcattt ccaaaatcta tcatttaaca aaaagttaat tttatggcta
    16921 atcgagataa atgcttctta ctataaaagg atatcggtaa ttaccatact tgaatatggt
    16981 gtttgatatt tgtgcaatca ttctcttaat gagccccatc ttgggagcag ctatttggca
    17041 tgcgagagca tgtttgggag ttattatttt gctttgtact ttgctcttaa ttttttctta
    17101 atgatcgttt tttataatgc aaaggttatt tttattcaag tttgtaaata tgtgaggttc
    17161 cttatgaaat tgcttcggtt tcatgaatta aaatcattgc caagcatgga cgagaaagca
    17221 ttagaattgt tgataaaggt tttaggcaac aaaggcatta gaaaactaat aaaatctgcc
    17281 gacggcaagc ctatttcacg tgaaataatg attcatgagt ttggaataga ttgtcaaatt
    17341 ctatttatta ccaccgaggc gtctcttaag ccaatcatcg ttcccactga aaacaagatt
    17401 tcaggaggtg gcaaatctta ttgtgagcaa tttaaagttt atgctttgga tgatggaaaa
    17461 acttatttcc taaaatccgt taagatcaat gctgagagtt tgacagagtt taccaatgag
    17521 acagacacct taagcaaatt gggtcgcttg gtaggcactt tttttaatga acaaactcag
    17581 gtgcattata ttctcacaac ttttataaaa gggattgatt tatcccggta taaaaacgcg
    17641 cttcctctta atattaatct aaaacatttt tgggaagttc tcggtattat gatttctgta
    17701 tgccatcagg tgaaacaatt ccatgaatta ggcttaattc accgcgatct taaacctgga
    17761 aatattatgc tggatgcgga tatgcagtgt catttagttg atttcggcag cagttcttct
    17821 gataaagaac ctaaaccggt atcatgggga accgcaagct atttagcacc agaacttaat
    17881 gcacaagagg attttattgc tttctctcag gctagcgatc tttttgcttt ggcttattct
    17941 ctagatgaac tttttaatcc attcaggcag gttaaatttg cgaaagtgga tataggaatt
    18001 aaaaataagc atcttgtgct tttgcacgct gaaatcgagg catgtattac tgggctcatg
    18061 tcaaatgaaa catcagtacg aacactttat ttttctcgca tcctgcaatt acaaagagtc
    18121 cctgagagtt tcaaatcgag accagaagca tttacctata tgattatgct gctcactcaa
    18181 tggaaaagct gttatgaagc accggaaatg aacaaagagt tagatgaaat cattgcagaa
    18241 ataaaagtgg cttatgaaaa ccatgaacaa gatgtcgtaa agattataac attgcttgag
    18301 cagttatcta aagcggacgg gctgcttaat tctcataaag ccttgctctc tgttttgatt
    18361 aaatcgcttg ctaatgtcca gcaacaagag ctcggacaag acgacatctt gccgcgtcgt
    18421 tttgaatcag acgttgtgag ccgcctgata aaaactccaa cagctaaaat gatggcagcg
    18481 attaaacaag tttcagatgc tatcgtaaaa atcctggaac aatatgaaca ttcccctgcc
    18541 gaatcttttg agcatgcggt tttacaagat tttctcgaac aaatggttca atcggatatt
    18601 ctttttggtg cccccaaaga gaaaattgag atttccgata ttataaaaat actcaaggca
    18661 aacaggccgg aagatttcgt acaaattcag catatttctt ttaaatttgc ccaagttgct
    18721 ttacgcgatc ttgccttgca tgacctgcca aaacatgagc caaaaggcgc ttttttagaa
    18781 atattgaaaa aatatcaagc aaaatatgcg cctgaagaga catcactttt tgagctctat
    18841 catcacatgg caggtgttag agatcacaac ccatcgaaag taaaaaaagg ggagggatac
    18901 cgctttagag ctttcatttg tgatgagctt ttttatggag atgaaatttt tactacgggg
    18961 gataatcgtg gccgcgaagg caagcctaac caaagattac aaactaatca agttggttta
    19021 atgaagttcg aacattcagc ccacaccaaa ggactactga cgttaaatgg tcaatcctgg
    19081 tatgctgatt gtaagaccca gctaccaaac tacgattcaa ttcattatct ctcagccctg
    19141 aaaacggact gtccttatat tacggggcct tccgggatga cttcattatt tatgaatatg
    19201 atgtttcttc tgctcaatcc taaggatcaa gatgtcatcc tctcttacag ccttggggta
    19261 atgacttatg tagttggtgc tggctaccac agcattaaag aaattcttat tccgatggtc
    19321 aagtgcgtag gtattgtgcc tgattatcca cagcataaag gaacagagtg tcttacagcc
    19381 ccgcctttat ataaccatta ctttaaggca atagaggagt ttgataaaga gtttgctgag
    19441 gttcatgaaa aaatttggca ggaatacctt gcatatttcc gtatgactta tatgcccgtt
    19501 tgtatgcgta gtcattgcct gccacatcaa gtcccctcaa tggaggatat aagctcagaa
    19561 gtaaaagaaa tgcttgaaat tgtgtctaag tctgtgaaaa attgcttaga agaacataaa
    19621 gttaagcgta ctggcgatat ggggttgttt ctttcaacac catgtgggca aacaaaggcg
    19681 cttgaggtct taagtcatgt ctgtcaacaa caggtagggc taaccgcaat tttcaggcag
    19741 atccaggcgt attttaaagg aacttttgag acagaagaag gaataattat aaatagagaa
    19801 atgcaactta aatttattgc tcatttcttt gatgtattga aggaaagccc aacactgctt
    19861 tcacaattca atttgacctt aaggatagaa aatacgattg gtgtgcatca gtgcgaattg
    19921 ggaagctcta agcaaaaaga gctcttcctg gagattaaga agacgtccat cgcagcaaat
    19981 gaagagcaga tggttgcaaa tcatgaaccc aggaaagaaa accgcaacgt catcattttg
    20041 ccatattaac ctcaaggaag gcgttctatg ttaagtaaag aaaaaattaa cccttttcta
    20101 aaaaatacaa aacgccaagg cagcgataac cttgctgcca tggctgatcc gacatcatta
    20161 aaaaacacgg gtgactaccg atgttttaat tcaaaaccag aggcttacac ttattttatt
    20221 tttttattaa atcaatggca gaccaattac attgacgaca aaagagaagc gatagctaaa
    20281 gcca
//
LOCUS       DACTJE010000043        20193 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.043, whole genome shotgun sequence.
ACCESSION   DACTJE010000043 DACTJE010000000
VERSION     DACTJE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20193
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.043"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(143..1165)
                     /locus_tag="I9760_001223"
     CDS             complement(143..1165)
                     /locus_tag="I9760_001223"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAT5042999.1"
                     /translation="MSRHLNVAIVGATGAVGETFLTVLEERNFPIKSLYPLASSRSVG
                     KTVTFRDQELDVLDLAEFDFSKVDLALFSAGGAVSKEYAPKAVAAGCVVVDNTSCFRY
                     EDDIPLVVPEVNPHRIADYTKRGIIANPNCSTIQMVVALKPIYDAVGISRINVATYQS
                     VSGTGKKAISELVAQVGDLLNGRPANVQVYPQQIAFNALPHIDQFEDNGYTREEMKMV
                     WETRKIMEDDSIMVNPTAVRVPVIYGHSEAVHLELKKPLTADDARALLAKAPGVTVVD
                     NLSKASYPTAIKNAVGHDDVFVGRIRQDISHPCGLNLWIVADNIRKGAATNAVQIAEI
                     LQKEFL"
     gene            complement(1275..2333)
                     /gene="aroC"
                     /locus_tag="I9760_001224"
     CDS             complement(1275..2333)
                     /gene="aroC"
                     /locus_tag="I9760_001224"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAT5043000.1"
                     /translation="MSGNTFGALFTVTTFGESHGPAIGCVVDGCPPGMSLTEADIQPF
                     LDKRKPGQSKYTTQRREEDKVQILSGVFDGKTTGAPIALLIQNTDQRSRDYEDIKNLF
                     RPGHADFTYHYKYGHRDYRGGGRSSARETAARVAAGAIARLYLKRYLNLDIIGYLQQM
                     GDLKLQFENENEINKNPFFCPNNKQIQELADYVDRLRRQGDSVGARVKILARGVPTGL
                     GDPVFDKLDATLAYAMMSINAVKGVEIGAGFNAVEQLGSHHRDQMTAKGFLSNHAGGI
                     LGGIATGQPIEVSIALKPTSSITTPGQTINTEGEEVTVVTKGRHDPCVGIRAVPIAEA
                     MMALVLMDHYLRHKAQCK"
     gene            complement(2345..3277)
                     /gene="prmB"
                     /locus_tag="I9760_001225"
     CDS             complement(2345..3277)
                     /gene="prmB"
                     /locus_tag="I9760_001225"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAT5043001.1"
                     /translation="MVMVDTLVSSYFSKETEELVTILDFLRFSLSCAENAQLFYGHGT
                     DNAWDDMRSLILRSLSLPYDVDPFLLNARLTTSERKHLCNQLDKRINQRVPVPYLIKE
                     AYFCDLPFYVDERVLIPRSPIGELINNQFSPWIEAERVHHVLDLCTGSGCIAIACCYA
                     FPEAQVDAVDISNQALAVATINCERHDVGDQLALIESDCFTALSGKQYDLIVSNPPYV
                     GKEEMQTLPDEYRHEPVLALETGNNGLAIIEKILKNAHAYLSEHGILVVEVGNSEEAL
                     CETYPMVPFTWLEMSNGGQGVFLLTKQQLEDYFK"
     gene            3442..4011
                     /locus_tag="I9760_001226"
     CDS             3442..4011
                     /locus_tag="I9760_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAT5043002.1"
                     /translation="MTDDFLSDEDKALFRHHMRSVKPLNEKTKRVKNPTQPTHEKKTT
                     TFKSQPVPKEEYFLSDFIRDTVQPDTILSFSEPGIPNQRFRALKNGQIPWEAKLDLHG
                     LKAEAARQALYDFIQTQSLNNKRCLLIIHGKGGHQGAPPVIKNLINRWLPQFSQVLAF
                     HSALPKDGGLGAVYVLLKRNREKDEQGNY"
     gene            4084..4503
                     /locus_tag="I9760_001227"
     CDS             4084..4503
                     /locus_tag="I9760_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAT5043003.1"
                     /translation="MEKLGQFIINHWQLWLAFVVVVFLIFINEILSQRKKAKEVSPQS
                     LVDLMNNGNAIVIDIRDKESFKNGHIINSMNVNPDDFEQQKMSKYKDKPIILVCARGL
                     QSPTLAMKIRNQGYQPLVLSGGIQAWQNADLPLVKGK"
     gene            4508..4762
                     /gene="grxC"
                     /locus_tag="I9760_001228"
     CDS             4508..4762
                     /gene="grxC"
                     /locus_tag="I9760_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAT5043004.1"
                     /translation="MNEVILYTTGYCPYCIKAKELLDRKKVIYTEIRVDLQPELREEM
                     IQKSGRRTVPQIFINGQAIGGCDDLYALEAQGTLNELLKK"
     gene            4772..5260
                     /gene="secB"
                     /locus_tag="I9760_001229"
     CDS             4772..5260
                     /gene="secB"
                     /locus_tag="I9760_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAT5043005.1"
                     /translation="MTEQLNTNQQNDEAQFMIQRIYIKDLSYETPNTPAVFQQQWEPE
                     LKLDLNTTTTQLDKNVYEVVLTVTATVMNQKTTAFLVEVKQAGIFTIQGAAASQLDHL
                     LHSFCPSILFPYAREAITSQVIRGSFPQLVLAPINFDALYMQQLEEKQKATGAKDETV
                     SH"
     gene            5262..6251
                     /locus_tag="I9760_001230"
     CDS             5262..6251
                     /locus_tag="I9760_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAT5043006.1"
                     /translation="MNKKTIAMLGAGSWGTAVAIHLAKIGHKTLLWSHNPQHVALMAE
                     QHSNPAYLPGIPFPENLIPSDNLIECVQSADYVIIAVPSHAFAEIINKIPKPTQGLAW
                     LTKGVDPASHELLSQLVASRFGVDFPIAVISGPSFAKEVARFLPTALTLASNNTNYQK
                     KMHQLFHHDNIRVYLSDDLIGVQLCGAVKNILAIACGISDGLGYGANAKAALITRGLA
                     EMTRLGLSMGARQDTFLGLAGVGDLVLTCTDDQSRNRRFGLLLGREVPIPEAEHQIGQ
                     VVEGKHNAAQICAIANKNKVEMPICEQINALLHGIVHAQEAVNNLMSRPAKEE"
     gene            complement(6337..7155)
                     /locus_tag="I9760_001231"
     CDS             complement(6337..7155)
                     /locus_tag="I9760_001231"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAT5043007.1"
                     /translation="MNSNKDLAIGVFDSGMGGLTVLRALRDKLPHESFVYLGDTARLP
                     YGTKSPDTVKQYAMQMAKILVERRIKALVIACNTATTAALPHLQSMLTDIPVLGVVAP
                     GAAAAVSATKNHRIAVLATETTIASRAYHDLIIKNLPEAVVSSRACSVLVALAEEGMV
                     TNAVAREALKHYLDDFSDEDAVLLGCTHFPVFKPLLSSLLPKDVAIVDSAQATAISLY
                     ELLQNQNLHNDNLEAPPRINYLVTDSINRFQKVGEIFLGEQLSLSDIELIDAMG"
     gene            complement(7248..10751)
                     /gene="ceg28"
                     /locus_tag="I9760_001232"
     CDS             complement(7248..10751)
                     /gene="ceg28"
                     /locus_tag="I9760_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg28"
                     /protein_id="HAT5043008.1"
                     /translation="MILEEYIRMAKNKEFFDALEEIAESAKNDETLRNELAKVLDDIL
                     KTDPSDPEAFRKIVAEHQEFWDEHDPSLMEFNEGRFFGKSRKQYLKSDDFLNSTDPTY
                     NFQKLHQFAAEQRVKLGLEKSDTDTLVAILKNNPEECRAYIESKKPGLGNFSEGNVHG
                     WLKEEYTPTIPPKAINKSTGVLSDEAIKRIKEQARDLLLLKLINSSGNTQLLKDLRDA
                     MSKPEAERAANALGFPTEGNGVLFLSREVVDALEERVEKLEQEAAKRGFDSYVQSLSH
                     NALLAKKNGLESTTAAGFKNSLDEPYKTYLPESEWERAQGVLGARYLQAVLSSGTQNL
                     KDALNAKDANALITELKKPALLGPHDYIDKAVTEENLGSLKKNMMKSFINNIKDETNL
                     KALDALKALDGAKNLDKFKEVLGKLGITPADWVKDTDLKDMKQWARARQFELEINRVS
                     SLGSGAHSKLMSTLTKLPVEKQREILAKPQQLRHLMNAYESHVAEHYLGKNASGIAEL
                     LTENKRLEGFRAIHNAEVARVLANFKPEITLNDKQVAAINQALTTANSNPNTYTQATD
                     YKILIDAIKTQSGSVNQKDFYNAFNLNDDGRAFTSSTPRKDEMSKQQQHNQHIYAEYN
                     STSNSGNKKLLAVLLSIEKPVTFSKDIVNRFLRPLKDSETPQDYADTLFGENPTNPAN
                     KKFKDDLLRELTPTVFNEIKNDLRKQELLDTNPAHVMTAIKALSTELESIKGITGPIR
                     TNADKLKFINDIDPVHLYNPTFQGTARSKAAQMKERYEGLSRDCGLVVDQLRRQVVAL
                     EGHLKSLPKEGEFKAAGLTLEQKAEIKKLRTDLEAELSAVREDLDFYKKIQGKLETIV
                     KEVDVAAKGKMHYYYNSEGIKRHPPVSRDQIPPLPNVPNPSLRSSTTATTGSNGRIQE
                     FLVGEKIPEGQIVVVDVSHKTAPKSGAPVETIGRYTQDNNVPDQVTSKKGEISKVPGS
                     KFEILQFPTQVPPPNPPSGDPLVEAKVNFSMAMAADILASLDSPPTKDKPIRLRGSNP
                     EELEYLYTALVILGEKNPKFKFNRDAIEVNSAVFHPDNVKGRLWGFSSNSLYSQVFTN
                     TGLTETQNIIQSKIKHMQQMTDEKFSPQKEREKVDSKVQEITDKQSKMKKELNPVHKT
                     TERTIEQEGPAPESPSTGMRK"
     gene            10873..11175
                     /locus_tag="I9760_001233"
     CDS             10873..11175
                     /locus_tag="I9760_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043009.1"
                     /translation="MRIKIILCGLILTNLTYAQGCIVGGQASQPRYVCFDGRTLDLTE
                     TGLVWNAKRGCFISSVPCCNYNATHYAYHPNPAKIGGAYARCRHDYPFTLGQMQTH"
     gene            complement(11179..12471)
                     /locus_tag="I9760_001234"
     CDS             complement(11179..12471)
                     /locus_tag="I9760_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HAT5043010.1"
                     /translation="MSPSESGELSIIQELSQRIVEAQRQIRILDSIKWDESVKQEFFK
                     FKGKKLPSIDKAYYDGKPLPFKVKDKQEEFRIILRDAQNQLGQYSTVTRLIRRQCEEY
                     SRAAQMLSLRGTPSFSEMAMELYGSPDDVFYVGGPRMCEMGSLLFDVLKSLQVQLQSQ
                     ADIKRYTPIQAQEILQERLGKFFDKHPGRVSVMVSDDMIADASAGADSIKLSQNAMFS
                     DRDLRYLEVHEGWVHVGTTLNGALQPNCFFLSKGSPSSSVIQEGLAVITEVVTFSSYP
                     GRMLKITNRVIALDKVTQGADFIDIYRYFLECGLSEEDSYNQSVRIFRGSTPTGGPFT
                     KDLSYAKGFVLIYNYIRFAIGQKRVDTIPLLFTGKLVLDDLPLLGELKEREILTDPVY
                     LPPPFQDLAALSAWMSFSLYLNQFDLNEIQKNFRFLLV"
     gene            complement(12481..12711)
                     /locus_tag="I9760_001235"
     CDS             complement(12481..12711)
                     /locus_tag="I9760_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043011.1"
                     /translation="MKKLSFIVLAALVLTACNSRYASNGENLYLQSRNGEALVVPPPL
                     TSANISNFYDLPQQTQDARVSIAPPVEDITTS"
     gene            complement(12726..13598)
                     /locus_tag="I9760_001236"
     CDS             complement(12726..13598)
                     /locus_tag="I9760_001236"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAT5043012.1"
                     /translation="MFSGSIVALVTPMRNDSVDVHHLRELVEFHIAKGTHALVAAGTT
                     GEAGTLSHSEKLLVIKTVIEQAKERVPVIAGTAMNATKDCIELTQQAMEYGAHAALIM
                     TPAYIKPTQEGLYLHYSHIAQSVAIPIILYNVPGRTACDMLPETVARLAKISNIIGIK
                     EATGQMTRLQQILRLCEGSIDVYSGDDLTAAQWLLAGAKGVISVTANVAAKLMAKMCD
                     LAMDDDQAGCLRIQEQLMPLHELLFVESNPIPVKWAMNKMGLIGGELRLPMTELSEKH
                     HQALEKVLKNLELI"
     gene            13810..14061
                     /locus_tag="I9760_001237"
     CDS             13810..14061
                     /locus_tag="I9760_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043013.1"
                     /translation="MKKIIPTLFFIGISTTTMVGCTNTQVGTAVGAAAGAGIGYGVSG
                     GSALGTAIGAGAGALVGGAIGQEQDRREYYYYSGYPYYY"
     gene            14339..15121
                     /locus_tag="I9760_001238"
     CDS             14339..15121
                     /locus_tag="I9760_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HAT5043014.1"
                     /translation="MKLKNKVAIVTGAASGIGKEIALVYAKEGAKVAIADLNLDQASH
                     VAEEIKSSGGQAMAVAMNVTDEAQVNQGVDKVADHFGGVDVLVSNAGIQIIESVDKLA
                     FSDWKRMLAIHLDGAFLTTKACLKYMYASGNGGSIIYMGSVHSKEASVLKAPYVTAKH
                     GLLGLCKVVAKEGGTHKVRANVICPGFVRTPLVDKQIPEQAKELGISEEDVIKKVMLK
                     ETVDGEFTTTDDVAQTALFFASFESNALTGQSLVVSHGWFME"
     gene            15128..16288
                     /locus_tag="I9760_001239"
     CDS             15128..16288
                     /locus_tag="I9760_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAT5043015.1"
                     /translation="MTRTKKSARQIEYRHIACSFQGGGALGAYQAGVLHALNEVGYHP
                     HWYVGTSIGAINAAIAAGNSDKERIEKMYQFWESIATPSAVNESTLLDDKISRKIQHL
                     IAAHSALLLGQPGFFVPRFPSPDFGWHNSPDLLSYYDTSPLHKSLEKFIDFDRLNNNK
                     LTRISVGAVEVSSGMMTYFDSYKQKIGPEHIMASGALPPGFPAVKVDGKYYWDGGISS
                     NSPVNYILNHNNQKQMLCFMIHLFDSFGMVPTSMDEVLKRKKDIEFSSRFIKLINLHK
                     EIHDLRNTIHILSNHISPEEKSKPEIRECIKKGRPSAISLVRFLYEHDETELSSKDYE
                     FSKKSIHERIQKGYDDGKNAIKKSPWNQSVPMTEGIALYDMSAKDTMKKYQD"
     gene            16318..17223
                     /gene="motA"
                     /locus_tag="I9760_001240"
     CDS             16318..17223
                     /gene="motA"
                     /locus_tag="I9760_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAT5043016.1"
                     /translation="MLIIVGYIVILLCVFGGFSLTGGHLAAIFQPVELLIIGGAAMGS
                     FIVGNNITVLKSVLKSLPATFRGQKSAKQASNDLLSLLYHLLSKARQQGLMSLENDID
                     EPDKSPIFTGYPNLMSNHHLIEFICDYLRLIITSNMQPFQLESLMDMEIESHHEEEMI
                     PANAITKLADAMPAFGIVAAVLGVVHTMESISLPPSELGVLIAHALVGTFLGILIGYG
                     FVGPIATAMEQNANQNQLMLQCIKVTILASLHNNPPIIATEFGRKVLFTSQRPSFNQL
                     NDEIKNAKTAQTTTQNSEATEATSS"
     gene            17233..18171
                     /gene="motB"
                     /locus_tag="I9760_001241"
     CDS             17233..18171
                     /gene="motB"
                     /locus_tag="I9760_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HAT5043017.1"
                     /translation="MSEINDISDPINDDLEKKDKREKKEKTTHYTPIIRKIKKNDHKH
                     HGGSWKIAFADFVTAMMAFFLLMWLVASLNKAQKAGIAEYFKQPMKVALFGGESMGNR
                     KATIKGGGPNIEDTDGQVSATNKPLINESIAKDKEIVESQANEIKKLEELKSEINLSM
                     EKDPSLAGLKKQLLMDVVSDGLRIQLIDNQKKPMFDVGSDKINPEIEPILANIAKLLN
                     SVPNKITIQGHTDANPYHNPEELEYTNWELSAQRANAARRALIKAGMDENKVMTVTGY
                     SSTILLDKKNPYNPENRRISIIVMKKGAENKIKVNK"
     gene            complement(18237..18710)
                     /locus_tag="I9760_001242"
     CDS             complement(18237..18710)
                     /locus_tag="I9760_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043018.1"
                     /translation="MFLEPGLINGVVMKRIILFMIAWSLLNSAYSSHSLPSHASILFT
                     LANTERSNSCPALLHNAKVVVDYDYNFERNMGLAHLRQLQSTKWSETLHPLGLSNYYA
                     FMSDMKPKAVQIEGGEVTIYRIIFHLYKNGDSRIYLMINQDGECIMSSDVVNVNL"
     gene            18955..>20193
                     /locus_tag="I9760_001243"
     CDS             18955..>20193
                     /locus_tag="I9760_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAT5043019.1"
                     /translation="MSSNESAAIVSPIVNQKLSQWKKQDTIWMLSLYGTAIGAGTLFL
                     PIDAGLNGILPLIIMTILAFPMTYYSHRALCRFVLSGSSSHNDITDVVEEHFGSFAGK
                     MLTILYFFAIYPILLMYSVAITNTTESFLINQMGITPPPRALLAIILILGLMGIVRLG
                     QEIIVKSMSVLVYPFATILFLLSLYLIPNWNNAIFIQSNSLHANGGHGLLMTLWLIIP
                     VMVFSFNHSPIISSFAVNQKQQYGSQADEKSSLIMKYSHLMMVFSVMFFVFSCVLSLS
                     PQDLLQAKQQNISILSYLANHFNTPIMAYIAPVIAFIAISKSFLGHYLGAKEGLSGII
                     VKSLKSTTGKTINKNSLHRIIEIFMILTCWVVATINPNILKMIETLGGPIIAVLLFIM
                     PMYAIAKVPAMKKYNNQISNV"
BASE COUNT         6130 a         4298 c         3742 g         6023 t
ORIGIN      
        1 tcaaaattta acagtcataa tagaagtata acttaggata taaaaagtat ggcatttttt
       61 ctaatataac ctgcatgacc ttatgggggc atacaggttt tctatcaaga tactggacct
      121 atgctattgc ggggtcggag cttcacagaa actctttttg cagtatttca gcaatttgta
      181 ccgcgttagt tgccgcacct ttgcgaatgt tatccgcgac tatccacaaa ttgagtccac
      241 aaggatgaga aatatcctgt cttatgcgcc ctacaaaaac atcatcatgc ccgactgcat
      301 tcttaattgc tgtgggataa cttgctttag aaagattatc cactacagta acgccgggtg
      361 cctttgccaa aagcgcgcga gcatcgtcag ccgtcaaagg cttttttaac tccagatgaa
      421 ccgcttcaga atgcccataa ataacaggaa ccctgacggc tgtagggtta accataatgc
      481 tgtcatcttc cataatcttg cgggtttccc agaccatctt catctcttct cgggtataac
      541 cattgtcttc gaactgatca atatgaggaa gcgcattaaa agcaatttgc tgaggataaa
      601 cttgaacatt agctggtctc ccatttaaaa gatcgcctac ctgagcaacc agctcgctga
      661 tggctttttt gccagtcccg gaaacagact ggtaagtcgc cacattaata cgactaatac
      721 caacagcgtc atagattggc tttagggcaa cgaccatctg aatggtagag caattagggt
      781 tagcaataat gcctctttta gtataatcag cgattcgatg aggattcact tcagggacaa
      841 caagaggaat atcatcctca taacgaaaac aggaggtatt atcaaccacc acgcacccag
      901 cagctacagc ttttggcgca tattcttttg aaacagcgcc gcctgcagag aataaagcaa
      961 gatcgacttt gctaaaatca aattctgcta aatccaaaac atccaattcc tgatctctaa
     1021 aagtcactgt cttacctact gagcgagaac tcgctaatgg gtaaagggac ttaattggaa
     1081 agtttctttc ttccaaaacg gttaaaaagg tttctccaac agcgccggtg gcacctacga
     1141 tagctacatt caaatgtctg ctcattatga cctcatctta ccatcaatta acaatgtgct
     1201 agtcaagtac agttcctgct tgcgaagcag aaaacggact acgatatggc tatattttga
     1261 aacgttataa tttattattt acactgtgct ttatgccgta aataatgatc cattaacacc
     1321 aaagccatca ttgcctctgc aattggcaca gcccttatcc ccacgcaggg atcatgacgc
     1381 cctttggtta ctacagtaac ctcttcacct tcagtattaa tggtctgacc tggagttgtg
     1441 atgcttgaag ttggctttaa agcgatgctc acttcgatgg gttgccctgt cgctatgcct
     1501 cctaaaatac caccggcatg attactcaag aatcccttgg cagtcatctg atctctatga
     1561 tgacttccta actgctccac tgcattaaaa ccagctccaa tttcaacacc tttaacagca
     1621 ttaattgaca tcattgcata agcaagagta gcgtccagtt tatcaaatac agggtctccc
     1681 aatcctgttg gcactccccg tgccagtatc tttacccgag caccaacgga atcaccctgg
     1741 cgtcttaaac gatctacata atcagccagc tcctgaattt gcttattatt aggacaaaaa
     1801 aagggatttt tattgatttc attttcattt tcaaactgta acttcaaatc gcccatttgt
     1861 tgtaaataac caataatatc caagtttaaa taacgcttta aatacaatct ggcaatagca
     1921 ccggcagcga ctcgtgcggc tgtctcacga gcggaagaac ggcctccccc tctataatct
     1981 ctatgcccat atttataatg ataggtaaaa tcagcatgtc ctggacggaa caaattttta
     2041 atgtcttcat aatcccggga acgctgatca gtattctgta tcagcaatgc aatgggagct
     2101 cctgtcgttt tcccatcaaa tacaccagaa agaatctgta ccttatcttc ctctcgtctc
     2161 tgggtagtat atttggattg acccggctta cgtttgtcta aaaaaggttg gatatctgct
     2221 tcagttaaag acatgccggg aggacaacca tcgactacac atccgatagc aggaccgtgg
     2281 ctttcaccaa aggttgttac agtaaataac gctccaaagg tattccctga catgccttac
     2341 tcgcttattt aaaatagtct tctaattgct gttttgttaa taaaaacacc ccttgcccgc
     2401 cgttgctcat ttcaagccag gtaaaaggaa ccataggata agtctcgcat aaagcctctt
     2461 cactattacc tacttccact accaaaatcc catgttcact taaataagca tgagcattct
     2521 ttaaaatttt ttcaataata gccaagccat tatttcctgt ttccaatgcc aaaaccggtt
     2581 catggcggta ttcatccggc agggtctgca tctcttcctt ccccacataa ggaggattgc
     2641 tcacgatcaa atcatattgt ttacctgaca gtgccgtaaa acaatccgat tcaatcagtg
     2701 ccaattgatc gcctacatca tgacgttcac aattgatagt agcgacagcc aatgcttgat
     2761 ttgaaatatc cacagcatcc acctgagcct cagggaatgc ataacaacag gcaatcgcta
     2821 tacaaccact cccggtacat aaatccaaaa catgatgcac tcgttctgct tctatccaag
     2881 gggaaaattg attattgatt aactctccta ttggtgatct gggtataagc actcgttcgt
     2941 caacataaaa aggcaaatcg caaaaatagg cttccttaat taaataaggt acgggtactc
     3001 tttgattaat tcgtttatcc aattgattac aaagatgttt gcgttcactg gttgttaaac
     3061 gtgcattcaa caaaaaaggg tcaacatcat atggtaaaga caagcttctc aatatcaggg
     3121 atctcatgtc atcccaagca ttgtcagttc cgtggccata aaataattgc gcgttttctg
     3181 cacaagacaa actaaaccgt aaaaaatcca gaatagtaac cagttcttct gtttccttgc
     3241 taaaataact actcacaagc gtatcaacca taaccataga aaaagtacaa attgtacact
     3301 aaattaaaat ctatgtcctg tattctttgt taaggggtgc gatttaagac gccccttcgc
     3361 atggctcgat tttcacctaa cctaaagaat tacttcaaat taagttataa ttaaatatat
     3421 tcttttgatg cctgttaaaa aatgactgat gatttcctgt ctgatgagga taaagctcta
     3481 tttcgtcatc atatgcgttc tgtaaaacca ttaaacgaaa aaacaaaacg cgtaaaaaac
     3541 cccacgcaac ccactcatga aaaaaaaacc acaactttca agagtcagcc cgtacccaaa
     3601 gaagaatatt ttttatctga ttttattcgt gacactgttc aacccgatac tattttatct
     3661 ttttcagaac caggcattcc caatcagaga ttccgtgcac taaaaaacgg acaaattcca
     3721 tgggaggcca agctggattt acatggatta aaagctgaag cagctcgcca agcgctttat
     3781 gattttatcc agactcagtc actcaataat aaacgctgct tactcattat tcacggcaaa
     3841 ggaggccatc aaggcgctcc cccggtaata aaaaacctaa tcaatcgctg gctgccacaa
     3901 tttagtcaag ttctggcgtt ccacagcgct cttccaaagg atggaggatt gggagctgtg
     3961 tacgtcttat taaaaagaaa cagagaaaag gatgaacagg gcaattacta gtaacacttt
     4021 acttgcgtta aagcagtaat cttaggataa ttcgtcaaat taaaaaatat cttgagtaca
     4081 gctatggaaa aattaggtca gttcatcatc aatcattggc aactctggct cgcctttgtt
     4141 gtggttgtat ttttaatttt cattaatgaa atactctctc aaagaaagaa agccaaagaa
     4201 gtttcaccgc aatccctggt tgatttaatg aacaatggaa atgccatcgt aatcgacata
     4261 cgcgacaaag aatcattcaa aaatggccat atcattaatt caatgaacgt aaatcctgat
     4321 gattttgaac aacaaaaaat gagcaagtac aaagataaac ccattatcct ggtctgcgca
     4381 cgaggcttac aatctccaac cctagccatg aaaatccgca atcaaggtta ccaacccctg
     4441 gttttgagtg gaggaattca agcctggcaa aatgctgatc ttcctttagt gaagggtaaa
     4501 taaacagatg aatgaagtca tactctacac tacaggctat tgtccttatt gcatcaaagc
     4561 aaaagaacta ctagatagaa aaaaggttat ctataccgaa atccgcgtcg atttacagcc
     4621 tgaattacgt gaagaaatga tacaaaaaag tggccggcgt acagtgcctc aaattttcat
     4681 taatggccag gcaattggtg gctgcgatga tttatatgcc ctggaagccc agggaacatt
     4741 aaatgaactt ttaaaaaaat aggaataaaa catgactgaa caactgaata ccaatcagca
     4801 aaatgatgaa gctcaattca tgattcaacg aatctatatc aaagatttat cctatgaaac
     4861 accaaataca ccggctgttt ttcaacaaca atgggaacca gaactcaaac tggatctcaa
     4921 tacaacgact actcaactgg ataaaaacgt ttatgaggtg gttttaactg ttacagccac
     4981 tgtgatgaat caaaaaacca cagcattttt ggttgaagta aagcaagcgg gcatctttac
     5041 tatccaaggc gccgcagcaa gtcagttgga ccacttgctt catagctttt gccccagcat
     5101 cctgttcccc tatgctcgtg aagcaattac ctcgcaagtc attcgtggca gcttccccca
     5161 attagtactc gcaccgatta attttgatgc tttatatatg cagcagctgg aagaaaaaca
     5221 aaaagcaact ggagctaaag acgagactgt ttctcactaa gatgaataaa aaaaccattg
     5281 ccatgttagg tgccggctcc tggggcactg ctgtcgcaat tcatttagct aaaatagggc
     5341 ataaaacgct gctgtggagt cataatccac agcatgttgc cctcatggct gaacagcatt
     5401 caaatccagc ttaccttcct ggtatacctt ttccagagaa tttgattcct tctgataatt
     5461 taattgaatg cgttcaatca gcggattatg tcatcatagc agttccttct catgcctttg
     5521 cggaaataat caataaaatc cctaaaccaa cccaaggatt ggcgtggctt acgaagggtg
     5581 ttgatccagc gagtcatgaa ttattaagcc aactggttgc cagtcgattt ggggttgatt
     5641 ttcccatagc agttatttca ggcccatcgt ttgccaaaga agtggctcgc ttcctgccta
     5701 cagctctcac tttggctagt aacaatacga actatcaaaa aaaaatgcat caactatttc
     5761 atcacgacaa cattcgtgtc tatttaagtg atgatttaat aggtgtgcaa ttatgtggcg
     5821 ctgttaaaaa tatcctggcg atagcctgtg gaatcagtga tggcttaggg tatggcgcta
     5881 atgccaaggc agcgcttatt acccgtggac ttgctgaaat gactcgtttg ggattaagca
     5941 tgggagcacg gcaagatact tttctaggct tggctggtgt aggggacttg gtattgactt
     6001 gtacagatga tcaatcgcgc aatcgccgct ttggattgct cttgggtcgt gaagtgccta
     6061 ttcctgaagc cgaacaccaa attggccaag tagttgaagg gaaacacaat gccgctcaaa
     6121 tttgcgctat agccaacaaa aataaggtgg aaatgccaat ttgtgagcaa attaatgcgc
     6181 tattgcatgg tattgttcat gcccaagagg ccgtgaacaa cttaatgagt cgtcccgcca
     6241 aggaagaata acctttgccc ttgcaaagaa tcatattaaa ttaggtttag tgcaccagga
     6301 caccaggacg caggtgatac tggtggattg tcgacattaa cccatagcat caatcaattc
     6361 aatatcactc aaactcaatt gctcacctaa aaatatctcg cctaccttct ggaacctatt
     6421 gatagaatct gttaccaaat agttgattct ggggggagct tccaaattgt cattgtgcag
     6481 attttgattt tgcaataatt cataaagaga aattgcagtc gcctgagcag aatcgacaat
     6541 tgcgacatct tttggcaata agctggatag caagggttta aatactggaa aatgagtgca
     6601 ccccaataac acagcatctt catccgaaaa atcgtcgaga taatgcttca atgcctcacg
     6661 agcaactgca ttagtcacca taccttcctc tgccaaggcg actaaaacac tacatgcccg
     6721 actactgaca actgcctcag gcaaattctt tatgatcaaa tcatgatagg ctctggaagc
     6781 aatggttgtt tctgtagcca gaacagcaat acgatgattt ttggtagctg acacagcagc
     6841 ggctgcacca ggtgccacca cacccagaac cggaatatcg gttaacatgc tctgtaaatg
     6901 aggtaaggca gccgtcgttg cagtattaca ggcaatgact aaagctttta tgcggcgctc
     6961 aaccaaaatt tttgccattt gcatagcata ttgtttgact gtgtcagggc tttttgtgcc
     7021 ataaggtaaa cgggcggtat ctcccaaata aacaaaagat tcatgaggta atttatcccg
     7081 cagagccctt aaaaccgtaa gtccccccat gcctgaatcg aatacaccaa tagccaaatc
     7141 cttgttagag ttcaattgat tactccttct tgtcatttgt ttcctggctc tcttatttgg
     7201 caaaatagct tcgttctacc aaaaatgaga gccaaagtca ttattattta cttacgcatc
     7261 cctgtactcg gactttcagg agcaggtcct tcttgttcaa tggttctttc agttgtttta
     7321 tgcaccggat tcagttcctt cttcatcttg ctttgtttat cagtaatctc ttgtactttg
     7381 gaatccactt tttctctttc tttctgagga ctaaatttct catcagtcat ttgttgcata
     7441 tgttttattt tagactgtat gatattctgg gtctctgtta atcctgtgtt agtaaatacc
     7501 tgactatata atgaattgct gctaaatccc cacaaccgtc ctttcacatt atccggatgg
     7561 aatactgcag aattcacctc aatcgcatca cggttaaact tgaattttgg attcttttca
     7621 ccaaggatca ccagggctgt gtacaaatat tccagttctt cgggattaga tccgcgtaat
     7681 cgtatcggtt tatccttggt tggcggacta tccagactcg ctaaaatatc agccgccatc
     7741 gccatggaaa aattgacttt agcttctacc agaggatctc cacttggagg gtttggaggt
     7801 ggaacttggg tcgggaattg taaaatctca aatttacttc cgggaacctt gcttatttct
     7861 ccttttttgc tcgttacttg atcaggaaca ttgttatcct gagtataccg tcctatcgtt
     7921 tccacaggag caccggattt cggtgctgtt ttatgagata catcaacgac aacaatctgc
     7981 ccttccggta ttttttcacc aacaagaaat tcttgtatcc ttccgttaga cccagtagta
     8041 gcggttgtac tcgatctcag agaaggatta ggaacattgg gtaatggcgg tatctgatca
     8101 cgtgaaacag gaggatggcg tttgataccc tcagaattgt agtagtaatg catcttccct
     8161 ttggctgcca catccacttc ttttacaata gtctctaact ttccttgtat ttttttataa
     8221 aaatccaaat cctcgcgcac cgcactcaat tcggcttcca aatcagtacg caactttttg
     8281 atttccgctt tttgctccag ggtcaagcca gcggccttaa attcaccttc tttgggcaga
     8341 ctcttcaaat gaccttcgag agcgactacc tgacgcctta attgatcaac aaccagtcca
     8401 caatccctgg acaaaccttc gtatctttct ttcatttgag ctgctttcga tctggcggta
     8461 ccctgaaagg ttggattgta taaatgaacc ggatcgatat cattgataaa ctttaactta
     8521 tctgcgtttg ttcttattgg tcctgtgatt cctttaatag actctaattc agtactcaga
     8581 gctttgattg ctgtcatcac atgagctgga ttggtatcta atagttcctg ttttctgaga
     8641 tcatttttta tctcgttaaa tacagttggt gtaagttccc gcaacaaatc gtctttaaat
     8701 tttttatttg ccggattagt tggattttct ccaaataatg tatcagcata atcttgagga
     8761 gtttcgctat ccttcagagg acgtaaaaac cgattgacta tatctttgga aaaggtgaca
     8821 ggtttctcta tactcaacag gacagctaat aattttttat tacctgaatt agaagtactg
     8881 ttgtattcag catatatatg ttggttatgt tgttgctgct tactcatctc atcttttcga
     8941 ggagttgagc tggtaaaagc tctgccatca tcgtttaaat taaatgcatt ataaaaatct
     9001 ttttgattta ccgagccaga ttgagtttta atcgcatcaa tcaagatttt atagtcggtg
     9061 gcctgggtat aggtattggg gttagaattg gcggtggtta atgcctgatt aatagcagca
     9121 acttgcttat catttaaagt gatttcgggt ttaaaattag ccaaaaccct ggctacctcg
     9181 gcattgtgaa tagccctaaa tccctcgagt cttttattct ctgtcaagag ctctgcaatg
     9241 ccggatgcgt ttttcccaag gtaatgttct gctacatgag attcataagc gttcattaaa
     9301 tggcgtaatt gttgtggctt ggccaaaatc tctctttgtt tttcgactgg aagcttagtt
     9361 aatgtgctca ttaatttcga atgggccccg ctgcctaaag aagacactct atttatttca
     9421 agttcaaact ggcgagctct tgcccattgc ttcatatctt tcaaatcggt gtctttcacc
     9481 caatccgctg gagtaatacc taacttcccg agtacctctt taaatttatc caaattttta
     9541 gccccgtcta atgcttttaa agcatccaag gcctttaaat ttgtttcatc cttgatatta
     9601 ttaataaagc tcttcatcat attttttttg agcgagccta aattttcttc tgtcaccgct
     9661 ttatcaatgt agtcatgggg tcccaagagg gcaggttttt ttaattcagt aatcaatgca
     9721 ttggcatctt tagcattgag agcatccttg agattttggg tgcctgatga taaaaccgct
     9781 tgaagataac gagctcccaa tactccttgg gctctctccc attccgactc cggtaagtac
     9841 gttttataag gttcatccaa actgttttta aaccctgcag ctgtcgtact ttctaacccg
     9901 ttttttttgg ctaatagcgc attgtgagat aatgattgga cataactatc aaatcctctc
     9961 ttggcagctt cctgttctag tttttcaacc ctttcttcca gagcatcaac tacttctcta
    10021 ctcaaaaaca ataccccgtt tccttcagta ggaaaaccca aggcattcgc agcacgttca
    10081 gcctcgggtt tactcatggc atcacgcaaa tcctttaata attgagtatt tccagaactg
    10141 ttaatcaatt taagcaataa caagtcacga gcttgttctt taattctttt gatcgcttca
    10201 tcagataaaa caccagtaga tttgttaata gctttcggag gaattgtcgg ggtatattct
    10261 tcttttaacc aaccatgcac atttccttca ctaaaattac ccaaaccagg ctttttggat
    10321 tcgatataag ccctacactc ttcaggattg ttttttaaaa tagcaactaa agtgtctgta
    10381 tctgattttt ctaacccgag tttgactcgt tgttctgcag caaattgatg taatttttga
    10441 aaattatagg ttggatctgt agaatttaaa aaatcatctg atttcaaata ttgttttcta
    10501 ctcttaccaa aaaaacgccc ttcattaaac tccatcaagc taggatcatg ctcatcccag
    10561 aactcctgat gctcagctac tatctttcta aaagcttcag gatcagaggg atcagttttc
    10621 aatatatcat ctaaaacttt tgcaagctca ttacgaagag tttcatcatt ttttgctgat
    10681 tccgcaattt cttccagcgc atcaaaaaat tctttatttt tggccatacg gatgtactcc
    10741 tcgagaatca ttatgtgttc attttaagcc aaaaaactta agttatcctt aaattttgcc
    10801 gatattaata ccacagatga aaacctaagc tagctttata attagaagta tagcaaggat
    10861 aaggtgaata gaatgagaat aaaaataatt ctttgtgggt taattctaac aaacctcacc
    10921 tatgcccaag gctgtattgt tggcggtcag gcctctcaac ccaggtatgt ctgttttgat
    10981 gggcgaacgt tggatctaac tgaaacaggc cttgtatgga atgccaaacg gggatgtttt
    11041 atcagctcag taccttgctg taactacaat gccactcatt atgcctatca cccaaatccg
    11101 gccaaaatag gcggagccta tgcccgttgc aggcacgact atccctttac tcttggccaa
    11161 atgcaaactc attaatgttt aaaccaacaa aaatcgaaaa ttcttttgaa tttcatttaa
    11221 atcaaactgg ttcaaataca gagagaaact catccacgca cttaatgcag ccaaatcctg
    11281 gaaaggaggt ggaagataca ctggatccgt taaaatctcg cgttctttta attcacctag
    11341 caaaggtaaa tcatccaaaa ccagcttgcc ggtaaataat agtgggattg tgtcgactct
    11401 tttctgccct atagcaaagc gtatgtaatt gtaaattaat acaaatccct tggcatagga
    11461 taaatccttg gtaaatgggc cacctgtcgg tgtgctgcct ctaaatatac gtacggactg
    11521 gttatagctg tcttcttctg acaatccgca ctccaggaaa tagcgataaa tatcaataaa
    11581 atctgcccct tgagtcacct tatccaaggc aatcacccgg tttgtaattt tcaacatccg
    11641 acctggataa gaagaaaatg tcacgacttc agtaataact gccaagccct cctgaatcac
    11701 gctgctggaa ggagaacctt ttgacaggaa aaagcaattc ggttgcaagg ctccattgag
    11761 agtcgttccc acatgaaccc agccctcatg aacttccaaa tagcgtaaat ctctatcgct
    11821 aaacatagcg ttctgactta atttgatgct gtcagctcca gcagaagcgt cagcaatcat
    11881 atcatcgctg accataacac tcactcggcc aggatgcttg tcaaaaaact ttcctaatcg
    11941 ctcctgtaaa atttcctgag cctgtatcgg agtgtagcgc ttgatatcag cttgagattg
    12001 caattggacc tggagactct ttaatacatc aaatagcagg gaacccattt cacacatacg
    12061 cggtccaccc acataaaaca catcgtccgg gctaccatat aattccatag ccatttcgct
    12121 aaatgaaggg gtaccccgca aagataacat ctgggctgcg cggctatact cttcgcattg
    12181 acgtctgatt aggcgagtta ctgtggagta ttgccctaat tgattctgtg cgtcacgcaa
    12241 aataatacga aactcttcct gcttgtcttt gactttaaaa ggcagtggct taccgtcata
    12301 ataagcctta tcaatagaag gaagtttttt tcccttgaac ttaaaaaatt cctgctttac
    12361 tgattcatcc catttgatgc tatcaagaat cctgatttga cgctgcgcct ccacgatacg
    12421 ctgagacaac tcctgtatta ttgacaactc tcctgattct gatggcgaca tgatgaatcc
    12481 ttatgaagtt gtgatatctt ccaccggagg cgctatactc acccgcgcat cctgagtttg
    12541 ttgaggtaaa tcataaaaat tactaatatt agcactagtc aatggaggtg gtactaccaa
    12601 agcttcgcca ttacggctct gtaaatataa attttctcca ttactggcat aacgagaatt
    12661 acatgctgtt agtaccagtg ctgccaagac tataaaactc aattttttca caacaaaacc
    12721 ctcagttata tcaactctaa attcttcaac accttttcca aagcctgatg atgtttttca
    12781 gacaactccg tcatcggtaa tcttaattcc cctccaatta atcccatttt gttcatagcc
    12841 cattttaccg ggattggatt cgactccacg aacaaaagtt catgtaaagg cattaattgc
    12901 tcttgaatac gcaaacatcc tgcttgatca tcatccattg ccaaatcaca catttttgcc
    12961 atcaatttag cagccacatt agccgtgact gaaataacac ctttagcccc ggccaacaac
    13021 cattgggcag ctgtcaaatc atcgccactg taaacatcaa tgctcccttc gcataaccgt
    13081 aaaatctgct gtaaacgagt catctgtccg gttgcttcct ttattcctat tatattcgaa
    13141 attttagcca gtcttgctac cgtttcaggt aacatatcac aggctgttct tccgggaaca
    13201 ttatataaaa taatcggaat cgctacggat tgggcaatat gactgtagtg caaatacaat
    13261 ccttcttgag taggcttgat ataggctggt gtcatgatta aagcagcatg cgccccatat
    13321 tccatagctt gctgggttag ttcaatacaa tccttggtcg cattcatagc agtacctgca
    13381 atcacaggca ctctttcttt tgcttgttca atgactgttt tgatgaccaa aagtttttcg
    13441 ctatgtgata aagtaccagc ctcaccagtc gtgccagctg cgaccaaggc atgagttccc
    13501 ttagctatat gaaattcaac cagttctcgc aaatgatgaa catcgacgct atcatttcgc
    13561 atgggggtta ccaaggctac tatacttcca ctgaacatgt tgccctcaaa aattaatgat
    13621 ccatgatcgt atattactgg aaaacagaca cccgagtaaa tttttatata acactttagt
    13681 caactggaat ctctatcagc aattattatc ttcaatccca aatacatatt tgtttaaatt
    13741 ttttacatat ttagcttatt ttgtactatt cttgatatga agacaacaac ttacaaaagg
    13801 aagtttacta tgaaaaagat tataccaacg ttgtttttca tagggatatc aaccactacg
    13861 atggtaggtt gcacaaatac tcaagttgga acagcagttg gagcagctgc aggcgctggt
    13921 attggctatg gagtttcagg aggatcagca ctgggaactg caattggagc aggggctggt
    13981 gcattggtag gcggtgccat cggtcaagag caagacagac gtgagtatta ttactacagt
    14041 ggctacccct attattatta attctgacta gtcgactaaa tatattcctt aatcatttta
    14101 gcgatcataa gaacctggtc ttttcaagat agagaaaggg ccaggataat tcttgatttg
    14161 ggtaaaacca aaactcttgg tgaaatgata cagaaaagaa gcaattacct cccttaaaga
    14221 caactctcta aaaaacttaa acaattcatc aaagattttt gttagcaaat aataaggcat
    14281 tattaaaaat ccattgctat aatcaattca tggaaaacaa tgacaaggat agtaaaagat
    14341 gaaactgaag aataaagtcg ctattgttac aggagcagca agcggaattg gtaaagaaat
    14401 tgcgcttgtt tatgctaaag aaggggcaaa ggtagcaatc gctgatctga atcttgatca
    14461 ggcaagccat gtagcagagg aaatcaaatc atcaggcgga caagcaatgg ccgttgccat
    14521 gaatgtaact gatgaagcac aagtcaatca gggcgtggat aaagtagccg atcactttgg
    14581 aggggttgat gttttagtca gcaatgccgg tattcaaatc atcgaatcag tggataaatt
    14641 agccttttca gactggaaaa gaatgcttgc cattcatttg gatggtgcct ttttaactac
    14701 taaagcttgc ttgaaataca tgtatgcctc aggcaatggc ggcagtatta tttatatggg
    14761 ctcggtccat tccaaagaag cttctgtact caaagcacct tatgttactg ccaaacatgg
    14821 tctattgggt ttatgcaaag tagttgccaa ggaaggtgga actcataagg taagagccaa
    14881 tgtcatttgc ccggggtttg tccgtactcc tctcgttgac aaacaaattc cagagcaagc
    14941 aaaagaatta ggcatcagtg aagaagatgt catcaagaaa gtgatgctaa aagaaacagt
    15001 tgacggggaa ttcaccacca cagacgatgt agcacaaacc gctttatttt ttgcatcatt
    15061 tgaatcgaat gcgcttacag gacaatcatt ggtagtcagt catggttggt tcatggagta
    15121 aacaaccatg actcgaacta aaaaatctgc aagacagatt gagtatcgcc atattgcatg
    15181 cagctttcag ggaggagggg ctttgggtgc ctatcaagct ggagtattgc atgctttaaa
    15241 tgaagtggga taccaccctc attggtacgt gggaacctca attggagcaa ttaatgcggc
    15301 gattgctgca ggtaactctg acaaagagcg gatagaaaaa atgtatcaat tttgggagtc
    15361 tatagctact ccgtccgcag tcaatgaatc aactctcctc gatgataaga taagtcggaa
    15421 aattcaacat ttaattgctg ctcattccgc tttactcttg ggacagccag gtttttttgt
    15481 acctcgtttc ccttcccctg attttggttg gcataacagc cctgatttat taagttacta
    15541 tgatacttct cctttgcata aaagtttgga aaaatttatt gattttgatc gtctgaataa
    15601 caataaatta acccgaatca gtgtaggagc agttgaagta agtagtggaa tgatgactta
    15661 ttttgattcc tataaacaaa aaataggacc tgaacacatt atggcaagcg gtgcgcttcc
    15721 tcccggcttc ccggcagtta aagttgatgg taaatattac tgggatggtg gcatatcaag
    15781 taattcacca gtcaattata tcctgaatca taataatcaa aagcaaatgc tttgttttat
    15841 gattcatttg tttgattctt ttgggatggt tcccacctca atggatgagg tattaaaacg
    15901 taaaaaagac attgaatttt ccagtcggtt tataaaatta attaaccttc acaaggaaat
    15961 tcatgattta agaaacacaa ttcacatttt atcaaaccat atttctcctg aggaaaaatc
    16021 aaaacctgaa ataagggaat gtattaaaaa gggacgtcct tccgctattt cgctggttcg
    16081 atttttatat gagcacgatg aaacggaatt gtcttcaaaa gattatgaat tctccaaaaa
    16141 atctattcat gaacgcattc aaaaaggtta tgatgatggt aaaaatgcga tcaaaaaatc
    16201 gccttggaat caatcagtcc caatgactga aggtatagca ctgtatgaca tgtctgccaa
    16261 agacactatg aaaaaatatc aagactaatt ggacactaga tactatagga tagggatatg
    16321 ttaattatag taggttatat tgtaatacta ctctgtgtat ttggtggatt cagcctgaca
    16381 ggcggccatc tcgccgcaat atttcagcct gttgaattat tgataatcgg aggggccgcc
    16441 atgggttcct ttatcgttgg taataatatt accgttttaa aatctgttct aaaatctctg
    16501 cctgctacat ttcgcggcca aaaatcggca aagcaagcct ccaatgattt gttaagcctt
    16561 ctataccatt tactcagtaa agcaaggcaa caagggttaa tgtcgctcga aaatgacatt
    16621 gacgagccgg ataaaagccc aatttttacc ggttatccca atttaatgag taatcatcat
    16681 ttaatagagt ttatttgtga ttacctgaga ttgattatca cttccaacat gcagcctttt
    16741 caattggaaa gcttgatgga tatggagatc gaatctcacc atgaagaaga aatgatccca
    16801 gccaatgcca tcaccaaact ggctgatgcc atgcctgctt ttggtatcgt tgcagcagta
    16861 ttaggtgttg tccataccat ggaatccatc agcttacctc cctctgaatt aggtgtttta
    16921 atcgctcacg cgttagtggg aaccttttta ggtattttaa ttggctatgg ttttgttggg
    16981 cctatcgcta ctgctatgga acaaaatgcc aatcaaaatc aattgatgct gcaatgtata
    17041 aaagttacca tactggcaag tttgcacaat aaccctccta ttattgctac tgaatttggc
    17101 cgtaaagtat tatttacatc gcaaagacct tcatttaatc aattaaatga tgaaataaaa
    17161 aatgccaaaa ccgctcagac aacaactcaa aactctgaag caacagaagc tacatccagt
    17221 taggttacag ccatgagcga aataaacgac atcagcgatc cgatcaacga cgatctggaa
    17281 aaaaaggaca agcgggaaaa aaaagaaaaa accactcact atacccctat catcagaaaa
    17341 attaaaaaaa atgatcataa gcatcatgga ggctcttgga aaattgcctt tgctgacttt
    17401 gtaacggcca tgatggcctt ctttctactc atgtggttag ttgcttcatt aaacaaagct
    17461 caaaaagcgg gtattgccga gtattttaaa cagccaatga aagtggcatt gtttggcggg
    17521 gaaagcatgg ggaatagaaa agcaacgatt aaaggaggag gccctaatat tgaagacaca
    17581 gatggccagg tttctgccac caataaacct ttaatcaacg aaagcatagc aaaagataaa
    17641 gaaattgttg aatctcaagc taatgaaata aaaaaacttg aagagctgaa atcagagatc
    17701 aatttaagta tggaaaaaga tccatcgctt gctggcctaa aaaaacaact cctgatggat
    17761 gttgtgtctg atggcttgcg cattcaactg atagacaatc agaaaaaacc catgtttgat
    17821 gtaggctctg ataaaatcaa tccggaaatt gaacccatac tcgctaacat cgcaaaacta
    17881 ctcaattcag tgcctaataa aatcactatt caaggccaca ccgatgccaa tccttatcat
    17941 aatcctgaag aattggaata caccaattgg gaattatctg cacaaagagc gaatgcagca
    18001 agacgcgcat tgataaaagc tggaatggac gaaaataaag ttatgacagt cacaggttat
    18061 tcttccacta ttcttctcga taaaaaaaat ccctacaatc ctgaaaatcg gcgcattagc
    18121 atcattgtca tgaaaaaagg ggcagaaaac aaaatcaagg tcaataaata atttggcatt
    18181 cgaccaaggc tagccccgtt tttgagagct tttcattaaa attaatacta tttactttac
    18241 aaattaacat tcacaacatc agaactcata atgcattcgc catcttgatt aatcataaga
    18301 taaatcctgc tatcgccatt tttatacaaa tgaaaaataa ttcgataaat ggttacctct
    18361 cccccttcaa tctgaaccgc tttgggcttc atatccgaca taaatgcata ataattactt
    18421 aaaccgagcg gatgtagtgt ttcgctccac ttggtcgatt gcaactgtct taagtgagct
    18481 agtcccatat ttcgctcaaa gttatagtca taatcaacaa cgacctttgc attatgcagc
    18541 aaagccgggc atgaattact acgctctgta ttggcaaggg taaagagaat gctggcatgg
    18601 gatggaagtg agtgagaaga ataggctgaa ttcaataaac tccaagcgat cataaacaaa
    18661 atgattcgtt tcataactac tccattaatt aaacctggtt cgagaaacat tcttattaca
    18721 atacgaattc atttgcaaca atttaaaaga ataagatcgg aatgagcttc atgaattgtt
    18781 aagaaggcaa ggccattttc ctcgctcctt accttaaatt aatctcttgc tttgttcatt
    18841 tcagttggat atgatttagt tcgcaattgt tgaaatattg tctttgtagt gttaatattg
    18901 atcaacctgc gaggcgctat tttattagtt agtattaaaa cgaggatgtt tttcatgtca
    18961 tcaaatgaat ctgcagctat agtatctccg atagtcaacc aaaaactatc acaatggaaa
    19021 aaacaagata cgatttggat gttaagcctc tatggcacag caattggtgc cggaactctt
    19081 ttccttccta tagatgctgg tcttaatggc attttgcctt taatcattat gacaattctg
    19141 gccttcccca tgacttatta ttctcatagg gctttatgtc gttttgtcct ttcaggctcg
    19201 tcctcacaca atgacatcac cgatgttgta gaagaacact ttggctcatt tgcgggtaag
    19261 atgctaacta tcctgtactt ttttgcgatt tatccaattt tgcttatgta cagtgtcgca
    19321 ataactaata ccacggaaag tttcctgatt aatcaaatgg gaattacccc tccgccacgg
    19381 gctcttttag caatcatatt aattcttggg ttaatgggta tcgtgcgttt aggacaagaa
    19441 attattgtca aatcgatgtc ggtattggta tatccttttg ccactattct atttctgctt
    19501 tcgctttatt taattccaaa ttggaataac gctattttta tccaaagcaa ttccttacat
    19561 gccaatggag ggcatggact attaatgact ttatggttaa ttattccagt gatggtgttc
    19621 tcctttaacc attctcctat catttcatct tttgctgtga atcaaaagca gcaatatggt
    19681 agccaagctg acgagaaaag ctcattaatt atgaaataca gtcacttgat gatggttttc
    19741 tctgtgatgt tttttgtatt tagctgtgtg ttgagcctgt cacctcaaga tcttttgcaa
    19801 gcaaagcaac aaaatatttc cattttgtct tatctggcga atcattttaa tacccctatt
    19861 atggcctata ttgcaccggt tattgcattc atagccattt caaaatcttt tctgggacat
    19921 tatttaggtg ccaaggaagg tttaagtgga attattgtta aatcattaaa atcaacaaca
    19981 ggcaaaacaa ttaataagaa ttccctgcac agaatcattg aaatctttat gattctgact
    20041 tgttgggttg ttgctacaat taatcctaat attttaaaaa tgattgaaac attaggaggt
    20101 cccatcatag ctgtcttatt atttatcatg ccaatgtatg ccattgctaa agtgccagcc
    20161 atgaaaaaat acaacaatca aatcagtaac gta
//
LOCUS       DACTJE010000044        20063 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.044, whole genome shotgun sequence.
ACCESSION   DACTJE010000044 DACTJE010000000
VERSION     DACTJE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20063
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.044"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            50..910
                     /locus_tag="I9760_001244"
     CDS             50..910
                     /locus_tag="I9760_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAT5043020.1"
                     /translation="MKIKTVTRYLLLFSIFFLIGFNNVFAQKKAIAITIDDLPFVGEY
                     RNFHLNMMMNTMKDEKIPATGFIIAKEVRPDNWEILHKFREAGFGLGNHTFSHANLNK
                     LKAKEYIQEIKEADSLLMPVLTEPKYFRYPYLAMSSGSKKNKILCYLEKHHYQVAPIT
                     IDSKDFVFNQRLLSVPELGRRAYLDELKPFYLDFIWQQTLKAEEHTQYHHNPDQAQIL
                     LIHANLLNAYVLPDIINLYKQNGYTFVSLEDALKTFKENYHCHKTRILTKKPAGEETD
                     LNIESFMDWD"
     gene            1132..1839
                     /locus_tag="I9760_001245"
     CDS             1132..1839
                     /locus_tag="I9760_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAT5043021.1"
                     /translation="MNKVITESQKFTLRNIANMLFCVESSVLPAQSNIAQKIQTQGIM
                     YNDLCKDSFDTEAPLNSNPLSIAGFTLVELLVTLSVFAIILTLIVPSLRTMILNSRLT
                     SNIDSLVSSLNYARGVALDRAVNVAVCPLGSPGSTACGANWSSGWIVVTQPVAGAPTL
                     LKSHQTSVNDPVITSNVSSVVFDPHGLSTTQSNFTMCDNRGNAFARSAMVLATGFVQS
                     GTTPGQAVWNNGALNCP"
     gene            1852..2391
                     /gene="pilV"
                     /locus_tag="I9760_001246"
     CDS             1852..2391
                     /gene="pilV"
                     /locus_tag="I9760_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HAT5043022.1"
                     /translation="MINRMDTLAKDKGMTLLEVLISVVILAVGMLGIASMLIVSNKAN
                     NSSYAKLQAVQAVYDIFDRIRANSDAASNGSYNVSNIGSNGYPTSVSAPSVLCNASVC
                     NPNQLARYDTWYWLTRVVSQLPNGSGSITSALSGVAGNTVITVTVQWDDSKAQNELGA
                     SSASSGTANFVQFRVQSQI"
     gene            2388..3455
                     /locus_tag="I9760_001247"
     CDS             2388..3455
                     /locus_tag="I9760_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAT5043023.1"
                     /translation="MNIKYRLQQGFSLVELMIATLVGLILTYSILQIYLAQTQLYKAS
                     NSQDLIQSTENAISNLLIPMIRSAGFAGCGSIITAVSSLNAGGPRPLGILNTTPTILM
                     GYNGSGASFTITQSNPANSTNASHWSPALDSTLVGNAQQGSDVLVVLGSTSDSYPTGI
                     TAIDPGSNTFVVQGTSGMSLTSGMFGAVSDCGKTVIFQITGVAGTTITHDAGAGVLQN
                     ASSAFPVNFQTGSQFIPIQQTAFFVGQGQGGQSALMRASLVGNNWTIQPLVPGVEIMK
                     VQYGIGNNGVITRYVSANAVTDWAQVYAIRLGFLIAGQLGSGSLTTRQYNVLDTQVTV
                     PADNRLRHVFELTINLRNSIS"
     gene            3452..3964
                     /locus_tag="I9760_001248"
     CDS             3452..3964
                     /locus_tag="I9760_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAT5043024.1"
                     /translation="MNVSFTAQYSKQRGYVLITSLVLMSMLTVLALTQISQNTSQTRI
                     ATNATDSEISFEKTEGAINEAINLIINGTYNPQSFLASNNGMYLFDQSATTPLWKTIN
                     WSSGSAVINSFQGNTGAQAGYFLEKLPSVIMPGQSMKKPSNVYRITARSSGASGDTSV
                     LIQSTIQIQQ"
     gene            3977..7486
                     /locus_tag="I9760_001249"
     CDS             3977..7486
                     /locus_tag="I9760_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilY"
                     /protein_id="HAT5043025.1"
                     /translation="MDALKLCILIREGAQKAIRVLPRIFLAISLSASVSLYAVPLTIP
                     QIPLIIASPIHPQVLILIGNSQSMDGNLSGAIMTGSGSLSSGLSSLYNSSSPVNYTVP
                     AGFTPPVQGPDANGLAPYTVIQGGNKVDNSSSRLNVAKAGVKAIIENYMPTTDFALGT
                     YSTSNISTYNTWVYYMSPPGSNFVFTNTPVAGNRYVINPCYNYGSASSTVSSNCASIG
                     SLYGTSLVSSSQYLQIGGASDDPTINDVLYAGSGFPGVFVSFNGPSPATPYPPNFSLS
                     NYNNGNVLLSYSSTRPSIGSFATGPTNAGFVPFSQQVMYVQRGFGYYSNQSFNTGNMR
                     VNMTTAGTNPTTTSVTNAINAFLPHLRPETNSTSTTEIKAAAVQSPLAGLLTRSRSFM
                     KTVGTTSGNCPQKQYIILISDGLPTQDLQARYWPPLGSAAATGYGVTATFNADGSLNS
                     TNSQALSDAINEINALKNDGVLTFVIGMGAGVDPAVNPEAAATLRAMAVAGGTENYYP
                     ATSPQALVNSLNSILSNIQNGSFSTSAAAISSTRIDSSTVEYQANFITRDTPYQDWTG
                     NLTAIELDENTGQPTNIIQWSAQSTLDSLVSGTGWSTNRKIATWDPIAADGVPFRWAN
                     INATQQAQLQPSDLLGENRLEYLRGNATLEKRNGGTFRDRSHVLGDIIDSQVIYVGPP
                     SGPYLTASYISFAKANANRQPMLYVGANDGMLHAFNATTGEEFFAFIPNAVFNNLHQL
                     TSPLYNQNHLFFVNGSPESADVQFSDGSWHTILVGGENAGGKSIFALDITNPTNLSSE
                     PGVANAVLWEFADTDLGFTYSKPQIGQIRSGSPSSLNFAVFFGNGYNNTNNKSVFYAV
                     DPQTGTLLRKIDLCAAVPSACNVNLPQGLSTVALGQKDGLQADPVTVVYAGDLQGNLW
                     AIDVSSADPASWSARVLFQARDSAGTPQPITTPPLVTLNPNYPRNPGLFVVFGTGQLL
                     TTSDLVTTQRQTIYGVWDKPLSSVPYVRANLQQQTLTLVNSATSGLSTNIITATATTI
                     NWSNKVGWFADLPIAGQRLITTPELINGAFIATLNTPPLNSCGFGFTSMLLELNFLNG
                     GAFQYARFDISGDGGFDIADQYNGAYPVGIGLSNSYANSPTVLGPNKDNNLVILITQS
                     DGTQTTVIAPNITPRKIGWWQIQ"
     gene            7498..7947
                     /locus_tag="I9760_001250"
     CDS             7498..7947
                     /locus_tag="I9760_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAT5043026.1"
                     /translation="MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQY
                     IQKSRRADAHATLTQDQIILERCYSQNFSYAAACGALPAFPQTTPNGYYTINISNLTA
                     TTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG"
     gene            8025..10232
                     /locus_tag="I9760_001251"
     CDS             8025..10232
                     /locus_tag="I9760_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAT5043027.1"
                     /translation="MEIFCFLIGVLYAYTYNIYLTIAIPLLFYITPRYPIVLFFIAGC
                     GFALIHQWIVSPKGMPDTQVITQAQLEGTIASIPMQHPNKTQFHFAIEKINNNPAQGL
                     IQLNWYNKPPVLRAGQRWQLSVKLKKPRNFHNPGGFNYVRYLAARHIHWTGYIRYRNN
                     KLISNQPPSFSWLALREKIGDALTKLAPDTETAGIIEALTLNVTHHISSDHWNLFRRT
                     GTIHLFGISGEHIALVSGIFFIVMRWIWSINYRCCLFLPANTIASIGGLLAALPYAFL
                     AGFEPPVQRALIGCVLYTIYAIGKQRFTPWQIWRYALFLVLCIEPHAVFLQGFYFSFL
                     AVACLLLTHQRWPLKNYKKTLALQLSCLIGLMPLTLHWYSYGSINGFLANLFAIPLVS
                     FLIVPLALMTMITSPFHWSWILMKPLAWLVALLLQGLTYVEQIAGINITWSITSVTMM
                     LAMTAGLFLRVLLPVKPFKHLAILWIILPLFPPRTAIYPGEALINILDVGQGLAVAIQ
                     TQHHVLLYDTGDSYFQGSDLGQMAIIPFFRSIGIKTIDKIVISHPDKDHKGGLNSLEK
                     EMKVNQLIVNDPYFYKRGSNCHSYPQWQWDGVSFRFLPIRESFNKKNNNSCILQVSNS
                     AGRVLLTGDIEKAAEEYLVRNYGSQLASEILVVPHHGSKTSSSYRFLLEVNPLYAIAS
                     LGFDNRFKFPHSKTLQSMKTLGITFFRTDECGMAAIKLPASGKINKPTCMQAV"
     gene            complement(10258..11565)
                     /locus_tag="I9760_001252"
     CDS             complement(10258..11565)
                     /locus_tag="I9760_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAT5043028.1"
                     /translation="MHTQRGLEVTTGSIEAVDSINYFHEQVLSSGQNAIQILDSAKKH
                     TDNLLIQTYAAAFYLYAQEDVANEQASDYLQSAFRLLTSANEREKLIFEAVTNWSKRD
                     YANAISLLVTLLERYPRDTLALKFMEWLFYCTGQAFNAEYFLKVCDKCAPENQDESHF
                     LAIHSFALELCGQYSKAKEMAEEAITMNLLTPWAHHTLAHVHLLTSDITGGINRLRDL
                     QKTWEDILPLLKGHNTWHLALFYLANRNEEEVKKLYPHISGALPDTVLEQLDTISLLW
                     RMDMAGLPQDRLLNQVVDHLSTHPLEYYTGFTNAHFIYCLVKSGYKNEADESLKRMKF
                     YACSPSSDALWGDVVLPLCQGIYAFADADYKTALMLMEPVIGECAQLGGSDAQIELFF
                     QTYLLVLIHNKQKDKALQFFTEHLKYYNNTPLSDWWFQSANKA"
     gene            complement(11766..12143)
                     /locus_tag="I9760_001253"
     CDS             complement(11766..12143)
                     /locus_tag="I9760_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043029.1"
                     /translation="MMKKINLFKLLMIFVLGTSALQAAGLSTQRIEQLSNARVTVWKT
                     IIYPSSKQVLPMHRHDNDRVLVALTDGTLKITNNKGKVHYLKLKKGKSYYLTKDAPNE
                     LHNDENVTNHPIQVMVIQLNDKA"
     gene            12280..12672
                     /locus_tag="I9760_001254"
     CDS             12280..12672
                     /locus_tag="I9760_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043030.1"
                     /translation="MLTTFLATVIGWYLVIVSLFLLFHNEHAKSAMAEIIAHRGSQLI
                     MAIVTLITGLLLVVSHNIWVMGWPVVITLFAWLVLLGGLIRFLCPDTAVRIGQSFVDN
                     PMRLKIAAVIFFIIGLFLLFNVYYVYLK"
     gene            12798..14726
                     /locus_tag="I9760_001255"
     CDS             12798..14726
                     /locus_tag="I9760_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043031.1"
                     /translation="MTRAQTCPHQTTVISNLKIIPLTLGWLLKSALLIIIIAVFIPLS
                     PKMPAPGLDASWAVGLNQAVAQGLAFGKDIFFTLGPYSSIYTKFYHPATDFMMVGGSL
                     YLALCYWIAIILLMQGIKWRWSIAFAILLFSMIYARDSLFFSYSLIIGLLIHKEISGK
                     ETSFKSNFCTLFALLFTPFGLFPLIKGSLLILSIITVLLCSLFLILYKQRNLAVLCLA
                     SPVVSILFFWIASGQSIFNLPAYVSNTILLASGFTEAMAIEGDNKELIFFGVTSSCIL
                     LSILLQKQLSPLSKAFLFCIYLVFLFLSYKTGFTRHFGHAFIAGTSVLLASFLLPYFN
                     SNNKVIVPLLALSLYTASYINGHYTKISVRDNFISTYTAAYYGLKNRIQNKNWLKENF
                     SLTMNYLKARDDFPRLPGTTDIYSYNQTSLIASGMTWSPRPIFQSYSVFTAKMAEINT
                     NYLLSENKPDNILFKVEPIDNRIPSMEDGMSWPILINHYQPVRLENDFLFLRKKLVNK
                     PVTSLVPLKTEIHTFGEQVKIPDTDKPLFVEIEIQPTLWGRLATTFFKPHQLEANLEL
                     KNGTQRQYRLISNMAKSGFLLSPLIENSAEFGLLFSQNNYLAGKKVISFSINSKQVPS
                     SHWQTKYTIHYKIFASNN"
     gene            14886..16310
                     /gene="sidA"
                     /locus_tag="I9760_001256"
     CDS             14886..16310
                     /gene="sidA"
                     /locus_tag="I9760_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector SidA"
                     /protein_id="HAT5043032.1"
                     /translation="MSKKYRTPIRLSDKMKTLKKNLKDQLAQSKSLGEQIHAGTTAAS
                     TITTGVSHVFNVIDSFLSAYRQIPLVGGILQLVATIAKSISIVSDPEKPIAEKIISLI
                     LIAAIIALSITAITLGGLAAAIIGTVIASTVTIMEGLGFFGKIAEKFQLANSYKMKKE
                     FIDLVAKRIEPNNDKYNDLLEIRAIELQHELEKSYPNQIEQQQLKEELNFINAVLRKK
                     NITPGNNKENDAYKLNQLYAKRAEQLKTLVDKIALINPAGKRAEQNKLLSEIKSLQKE
                     ITKTDDSIEEIIAPLAKIERDNLKSTINLPLSYSTFAIAAASTLISIAGLLVVAGVIA
                     APPIMVPIMFAVGSVMAIIGLIKWTAEKMAEIEDAQLKAEETAKHEDAVLDEALNVYE
                     QQLNLELVSPSNPARQVKNILTVHDKVPALSPTETTVSSLDSRFTLFGSSSREHTKIA
                     EQKTTDTTNEQAPTTFDSISKPTR"
     gene            16400..17374
                     /locus_tag="I9760_001257"
     CDS             16400..17374
                     /locus_tag="I9760_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 family protein"
                     /protein_id="HAT5043033.1"
                     /translation="MKSREKFMARNKRLDRFHNAQNSNTCGYDQAFSEIQDGRKRSHW
                     IWYIFPQLENLGKSPSAKLYGIKDFNEACDYLKDPVLFKRYFELIKLIEEKLKNTPLP
                     HLMGGFPDDRKFISSLTLFQATSNYLSSKDTDSQNNYHELYETCNRVFKQIAPQGYSP
                     CQQTELLVKHQSNSPVKKTTEKSSQPPQVTIFSPKRSKPNTAHPILKDLAQYINKRGN
                     EWSYHYNFLGLVAFVYFIQDLILGTDHFNHKAREVKLTAASKLQKILDPQCHERCNLN
                     PSERKALSDGRLGEIINKHGGLDKILTEAPRKPPKTGHDQEINISPFS"
     gene            complement(17480..17860)
                     /locus_tag="I9760_001258"
     CDS             complement(17480..17860)
                     /locus_tag="I9760_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAT5043034.1"
                     /translation="MTQAAFGEFCWNELATSDVKKAKDFYGKVFGWQFREIESKEMTY
                     TLIQCNDKEMAGIWQIPKDQQSHIPPHWMAYIYVQDVLEALKKAKQHGAQEVKGITQA
                     GEMGRFAIIVDPTGAHVALWESFE"
     gene            18175..18747
                     /locus_tag="I9760_001259"
     CDS             18175..18747
                     /locus_tag="I9760_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAT5043035.1"
                     /translation="MSQNIILNRCEWVGQNKPHYELYHDTEWGIPVHDDEKHFEMLIL
                     EGAQAGLNWETILKKRDGYRRAFKQFNPQAVAQMTDDELNALLINPEIIRNRLKIFST
                     RKNAKVFLSIQQEYGSFDSYVWQFVNGAPIINRPESIRAIPATSKESDALSKDLKKRG
                     MSFVGSTIIYAYMQAIGMVNDHIVTCFRAK"
     gene            complement(18906..19997)
                     /locus_tag="I9760_001260"
     CDS             complement(18906..19997)
                     /locus_tag="I9760_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043036.1"
                     /translation="MKKYHQLLMGISLLLFGVCNYAGDTGPVENSDLFRVFIPNLKPG
                     YEFSATAFYLQPGADNLGWGVITTVLPIPSPNWQVVTFNPDYQVGFNLGGRYVFANSG
                     SDLQLNWSHLRTNDTDNVFVNPSSQWISPFSQTGTPPTPSGQITGVALLKLAHASLDF
                     SYDAVSLDVGKFINFGSKLQTRLFTGLSSAWIKEELISTFRGFSLPILSLNNTSTYKG
                     VGPRLGFNNSYNISHGFNLVGQLAGSVLYGRMQPAQYQFTGTSQQLIIAGIFVNREGL
                     ANPSVNQLVPALDAKIGLSYLYSLKQNWDLNFEAGYMGALYFNPLSSYETNTNVIALD
                     TGSLSTSSAKHTQSNFSVGGPYITVSLRT"
BASE COUNT         6222 a         4231 c         3699 g         5911 t
ORIGIN      
        1 aatagtgaca atatttttct caattgacct tacacatatt aaatttcgaa tgaaaatcaa
       61 gactgttacg agatacttac tgcttttctc aatatttttc ctgataggat ttaacaacgt
      121 atttgctcaa aaaaaagcca tagcaatcac tattgatgat ttgccctttg ttggtgaata
      181 ccgaaacttt catctgaata tgatgatgaa tacaatgaaa gacgaaaaaa tcccggcaac
      241 aggttttatc attgccaaag aagtcaggcc tgataattgg gaaatacttc ataaatttcg
      301 tgaagcaggt ttcggcttgg gcaaccatac tttttctcat gccaatttaa acaaactcaa
      361 agcgaaagaa tatatacagg aaattaaaga agcagattca cttttaatgc ccgtcctcac
      421 tgaacccaag tattttcgtt atccctattt ggcaatgagt tctggaagta aaaaaaataa
      481 aatcctctgc taccttgaaa aacaccacta tcaagttgcc ccgattacca ttgacagcaa
      541 agattttgtc tttaatcaac gcttattatc tgtgcctgaa ttaggaagga gagcttacct
      601 ggatgagttg aagccatttt atctggattt tatctggcaa caaacgttaa aagctgaaga
      661 gcatacgcaa tatcaccata atccagatca agcacaaatc ctgttaatcc atgctaactt
      721 acttaatgcc tatgtcctgc ctgatattat taatttatac aaacaaaatg gttacacgtt
      781 tgtgagtctg gaagatgcct taaaaacatt caaagaaaac tatcattgtc ataaaaccag
      841 aatccttaca aaaaaacctg ccggagagga aacggattta aacatagaat catttatgga
      901 ttgggattaa taagcaccat gaagccttca gactgtaaaa tttttttgcc agaccaatgt
      961 ttctggcccc tatataattc tctctataca accttgctaa ccggactatc cttaaaattg
     1021 caatagctaa ctggaccaag catttgctag ccaattattt gtaaaaaatt taactaattt
     1081 gaactataat taacaatgta gtgggttcga tcaaacaata gggataatgc tttgaacaaa
     1141 gtgataacgg aatcacaaaa attcactctg cgcaacatag ctaatatgtt attttgcgtt
     1201 gagagtagtg tgttgcccgc acaatctaat atagcacaaa agatacaaac acaaggcata
     1261 atgtacaatg atctttgtaa agacagcttc gatactgagg cccctcttaa cagcaaccct
     1321 ctttccatag ccggattcac actggttgaa ttattagtaa ccctatctgt ttttgccatc
     1381 atcttaactc taatcgtccc ttcattaaga actatgattt tgaatagccg tttgacttca
     1441 aacattgact cactggtaag ctcattaaac tatgcgcgtg gagtggcact tgatcgggca
     1501 gtcaatgtgg cagtatgccc attaggttca ccaggttcaa cggcctgtgg agccaactgg
     1561 agctcaggat ggatagtagt gacccaacca gtagcaggag caccaacgct tttaaaatcg
     1621 caccagactt cagtgaatga tcctgttatc acttcgaacg tatccagtgt tgtttttgac
     1681 ccacatgggt tatcaacaac ccaaagtaac ttcactatgt gtgataacag aggcaatgcg
     1741 tttgcccgct cggcaatggt tttggccact ggatttgtcc aatccggaac aacacccggc
     1801 caagcagtct ggaataatgg agctttgaac tgcccttaac ggagatagaa tatgattaac
     1861 cgaatggata cattagcaaa agacaaggga atgactttgc tggaagtgtt gatatctgta
     1921 gtcatattag ccgttggcat gctagggatc gccagtatgt tgatagtcag taataaagcg
     1981 aacaattcca gttatgcaaa actgcaagca gtacaagcag tctatgatat ttttgacaga
     2041 attcgagcga attcggatgc cgcatctaat gggagttata atgtcagtaa tatcggcagt
     2101 aatgggtatc ctacgtccgt aagtgctcct tcagtattat gcaatgcatc ggtatgcaat
     2161 ccaaatcaac tggcacgata tgatacctgg tattggctca ccagagtggt gtcgcaattg
     2221 ccaaatggaa gcggttcaat aacgtctgct ctttcaggtg tggcaggaaa taccgtgatt
     2281 accgtgactg tacaatggga tgatagtaaa gcacaaaatg agttaggggc aagcagcgct
     2341 tcttcaggaa ctgccaattt cgttcaattc agagtacaga gtcagatatg aatattaagt
     2401 acagactaca acaaggattt tcattagtcg agttaatgat agccaccttg gttggactga
     2461 tactcactta ttcaatttta caaatatacc tggcacaaac ccaactttac aaagcatcga
     2521 actctcagga tcttattcaa agtactgaaa atgccatatc caatttactc ataccgatga
     2581 taagatcggc tggatttgcc ggctgtggat ccattatcac cgcagtttcc agcctaaatg
     2641 ctgggggccc aaggcctttg ggaatattga atacaacgcc aacaatcctt atgggttaca
     2701 atggcagcgg agcgagcttc acaataaccc aaagcaatcc tgcaaatagc accaatgcca
     2761 gtcattggtc acccgcatta gattccaccc ttgttggcaa tgctcaacaa ggtagtgatg
     2821 tgttggtagt attaggctct acctctgaca gctatccaac aggaataacc gcgattgatc
     2881 ccggcagcaa tacctttgta gttcaaggca ctagcgggat gtcccttacc tctggaatgt
     2941 ttggagcggt ctctgattgt ggtaaaaccg taatatttca aattaccgga gtagcaggaa
     3001 ccacgattac tcatgatgca ggcgctgggg tgctacaaaa tgcaagcagt gccttccctg
     3061 tcaattttca aaccggctcc caattcattc ccatccaaca aacggcattt tttgtgggtc
     3121 aaggtcaagg tgggcaaagc gcattaatgc gggcctctct cgttggcaac aactggacaa
     3181 tacagccatt agttcctggg gtggaaataa tgaaagtcca gtatggtatt ggcaataatg
     3241 gagtcattac tcgatacgtc tctgctaatg cagtaactga ttgggcacag gtttatgcca
     3301 ttcgtttggg atttttgata gcaggtcaac tcggatcagg aagcttgaca accagacaat
     3361 acaacgtttt ggatacgcaa gtcactgtac ctgctgataa ccgcttaaga catgtatttg
     3421 aattaaccat caacttgagg aattcgatat catgaacgta tctttcacag ctcaatattc
     3481 aaaacagcga ggttacgtcc tgataacctc tcttgtgctt atgtcaatgc ttaccgtttt
     3541 agccctgacc cagatatcac aaaacacatc tcaaaccagg attgcaacta acgcaaccga
     3601 ttcggaaatc agttttgaaa aaactgaagg cgccattaat gaagccatta atctgatcat
     3661 aaatggcact tataaccctc aaagttttct tgcttctaat aatggaatgt atttatttga
     3721 ccaaagtgct actaccccac tttggaaaac gattaactgg tcaagtggtt cggcagtcat
     3781 taacagcttt caaggcaata caggggcaca agccggctat ttccttgaaa aactgccgtc
     3841 cgtgattatg cctggccaga gtatgaaaaa gccaagtaat gtctacagaa taactgctcg
     3901 gtcatcagga gcaagcgggg atacttcagt actcatacaa agtacaattc aaattcaaca
     3961 gtaggttttg attatcatgg acgcgttaaa actttgcatt ttaatcagag aaggagctca
     4021 gaaagcaatt agagttctac ctcgaatatt tctagccatt tcattgtctg cctcagtttc
     4081 tctttatgcc gttcccttaa ccattccaca aatacctcta atcattgcca gtccaattca
     4141 ccctcaggtt ctgatactca taggaaactc tcaatccatg gatggtaatt tgagtggagc
     4201 aatcatgacg ggctctggtt ctctgtcctc cggtttaagc tccctttaca attccagttc
     4261 acctgtgaat tataccgtac cggctggatt tactccgcca gtacaaggcc ctgatgccaa
     4321 cggcttagct ccttatactg ttattcaggg aggtaataaa gtagacaaca gttcaagccg
     4381 actcaatgtg gccaaagctg gagtcaaagc gattattgaa aattacatgc ctacaacaga
     4441 ctttgcattg ggcacctaca gcacaagtaa catcagtact tataacactt gggtttatta
     4501 catgtcgccg cctggaagta attttgtgtt caccaacacc ccggtagctg gaaatcgata
     4561 cgttatcaac ccttgctata actatggttc ggcttcatca actgtttcaa gtaactgtgc
     4621 atccattgga agcttgtatg gtactagttt agtttcttca agccagtatc tacaaatcgg
     4681 gggggcaagc gatgatccaa ctattaatga cgttctatat gctggaagtg gttttcctgg
     4741 agtttttgtc agttttaatg gcccatctcc agcaacacct tatcctccga atttttctct
     4801 cagcaattat aataatggta atgtacttct ttcctattct tcaaccagac cctcgattgg
     4861 aagttttgct acaggcccca caaatgcagg ttttgtaccc ttttcacaac aggtaatgta
     4921 tgtgcaacgt ggttttggct attacagcaa tcaatctttt aataccggca atatgagagt
     4981 gaacatgact acggcaggaa caaatcctac aactactagt gttactaatg caattaatgc
     5041 gtttttgcct catcttcgac ctgaaacaaa ttctacatcg acgacagaaa tcaaagcagc
     5101 cgctgtacaa tcccctcttg caggattatt gacacgatct cgaagtttca tgaagactgt
     5161 cggtacaacc agcggcaatt gtccgcaaaa acaatacatt atattaattt ctgatggtct
     5221 acccactcaa gacctccagg caagatattg gccgccacta ggaagtgcgg ctgcgaccgg
     5281 ctatggcgtc accgcaacat ttaatgccga tggctcactg aatagtacta acagtcaagc
     5341 cttatcggat gcaatcaatg aaataaacgc tttaaaaaac gatggcgttc ttacttttgt
     5401 tataggcatg ggagctggcg tagatcctgc tgtaaatcca gaggcagctg caacccttag
     5461 agccatggct gttgcaggag gtaccgaaaa ctactaccct gcaacaagtc ctcaagctct
     5521 agtcaatagt ttgaactcca ttctctcaaa tattcaaaat ggatcttttt ctacctctgc
     5581 agcagctatc agctcaactc gtattgacag tagtactgta gaatatcaag ctaactttat
     5641 aaccagagat acaccctatc aggattggac tggtaatttg acagctattg aactggatga
     5701 gaatactggc caaccaacaa atattataca atggtcagct caatcgacac ttgattccct
     5761 ggtatcagga accggatggt caactaaccg aaagatagcg acttgggatc ctatagccgc
     5821 agacggagta ccgtttcgct gggcgaatat taatgccacc caacaggctc aactgcaacc
     5881 aagtgatctg ttgggagaaa accgattaga atacctgcgt ggcaatgcca ctctggaaaa
     5941 aagaaacggg ggaacattcc gtgatcgctc gcatgtattg ggagacatta tcgatagcca
     6001 ggtaatttat gttggcccac caagcggccc ctatttaacc gccagttaca tcagcttcgc
     6061 aaaagccaat gccaatcgac aacccatgtt atacgttggg gcaaatgacg gaatgcttca
     6121 cgcctttaat gcaaccactg gagaagagtt ttttgcattc attcccaatg ccgtgtttaa
     6181 taatctccac caactcactt ctcccctgta caaccaaaat catttattct ttgtcaacgg
     6241 ctctccggaa agcgctgatg tacaattttc cgatggttcc tggcacacca ttctggtggg
     6301 aggtgaaaat gcaggcggta aaagcatctt tgccctggac attacaaatc cgacaaacct
     6361 gagttctgaa ccaggagtgg ctaatgctgt gctctgggaa tttgcagata ctgacttagg
     6421 gttcacttat agcaagcctc aaataggtca aatcagatcc ggaagtccat cttcattaaa
     6481 ttttgccgtc ttttttggca atggatacaa caatactaac aacaaatcgg tattttatgc
     6541 cgtagaccct caaacaggaa cactactaag aaaaatagat ctttgtgctg ctgtaccttc
     6601 agcctgcaat gtcaatttac cccagggatt atcgacagtc gctttgggac aaaaagacgg
     6661 attgcaagct gatcctgtta ctgtagtgta tgccggtgat ttgcaaggca atctgtgggc
     6721 aattgatgtt tcctcggctg atcctgccag ttggagtgcg cgtgtattat tccaggcaag
     6781 agactctgct ggtacaccgc aacctatcac tactcctcct ttagtgacat tgaatcctaa
     6841 ttatcctcgc aatccaggac tttttgttgt gtttggcacc ggacaacttc tgaccacatc
     6901 cgatttggtc actactcaaa gacaaaccat ttatggagta tgggacaagc cgctttcatc
     6961 tgtgccttat gtacgtgcca atctccagca acaaactttg accttggtta actcggctac
     7021 atcaggttta tccacaaaca ttatcactgc cactgctact accatcaatt ggagtaacaa
     7081 agtgggatgg tttgctgatc tgcctatagc ggggcaaaga ctcattacta cacctgaact
     7141 aattaacgga gcatttattg caacgctaaa cactcctcca ctcaattcgt gcggttttgg
     7201 tttcacatcc atgcttctgg agcttaattt cttaaatggc ggtgcttttc aatatgcgcg
     7261 atttgatatt tccggcgatg gaggctttga tatagccgat cagtataatg gggcctaccc
     7321 cgttggaatt ggcttgtcca acagttatgc caactcacca actgttctgg ggcctaacaa
     7381 ggataacaat ttggtcattc tgatcacaca atcggatgga acacaaacta ctgttatcgc
     7441 tcctaacatt acgccaagaa aaataggctg gtggcaaatt caatagagga tacccaaatg
     7501 ctcagtcatg tacattttat gaagaatagc cgtatgaaac aatccgcgtt taccctggtt
     7561 gaagttctga tcagcatggt cattatgggc attctggttt caattgccta tccatcctat
     7621 ttacaatata tccaaaaatc ccgtcgtgcc gatgctcacg ccacattgac acaagatcaa
     7681 attattttag aacgctgtta ttcacagaat ttttcttatg ctgcggcgtg tggcgcctta
     7741 ccagcatttc ctcaaacaac gccgaacggg tactatacta tcaatatttc aaacctgaca
     7801 gccacaacgt ataccttaac tgcaacccct gttggaactc aagccaaaga taccgagtgt
     7861 gccagcatgt caattaacca ggccaatgta aaaacagcag tagattcctc cgctaatgcg
     7921 caaccagaat gctggaatcc cggttaatac tgtaaaaaat tctttacaca aaggattcaa
     7981 ttcgagtaga tttgccagta agggaaagtc caacttaatt tgaaatggaa attttttgct
     8041 ttttaatagg cgtactttat gcctatacct ataatattta tctaactatt gcgattcctt
     8101 tactctttta tatcacacca agatatccca ttgtcctgtt cttcattgcc gggtgtggct
     8161 ttgccctgat tcatcagtgg attgtatctc ccaagggcat gcctgatacg caagtaatca
     8221 cacaagccca attggaagga acaattgctt ccatcccgat gcaacatcct aacaaaaccc
     8281 aattccattt tgcaattgaa aaaattaata ataaccctgc tcaaggcttg atccaattaa
     8341 actggtataa caaaccacct gtcttacgag ccggtcagcg atggcaactt tcagtgaaac
     8401 taaaaaaacc tcgtaacttc cacaacccgg gaggttttaa ttatgtacgt tacctggcgg
     8461 ctcgtcatat ccattggact ggctatattc gttatcggaa taataagcta attagcaatc
     8521 aacccccttc tttcagttgg ttagcactac gtgaaaaaat aggggatgct ttgacaaaac
     8581 tggcgccgga tacagaaacg gcaggcatca tcgaagcatt gacgttaaat gttactcacc
     8641 atatcagttc agatcattgg aatttgttcc gaagaacagg aacgatacat ttattcggga
     8701 tttcaggaga gcacatcgct ctggtctctg gcattttctt tatagtaatg agatggattt
     8761 ggtcaataaa ttatcgatgc tgtttgttct taccggccaa taccatagcg agtatcggtg
     8821 ggctactggc cgccttgccc tatgcttttt tagccgggtt tgagcctcct gtgcaacgtg
     8881 ctttaattgg ttgtgtactt tacacaatat acgccatagg aaaacaacgt tttacaccat
     8941 ggcaaatatg gagatatgcc ttatttttag tcttgtgtat tgagccccat gccgtattcc
     9001 tgcaaggttt ttacttttca tttcttgccg tggcctgtct tctattgact catcaacgct
     9061 ggccactgaa aaattataaa aaaacactgg cattacaatt aagctgcctc attggattaa
     9121 tgcccttgac tctccattgg tattcctatg gttctatcaa tggatttctt gccaatttat
     9181 tcgccatacc tttagtcagt tttctgatag tacccttggc tttaatgacc atgataacga
     9241 gtccatttca ttggtcctgg atattgatga aaccattggc gtggcttgtt gccctgttac
     9301 tgcaaggact aacgtatgtt gagcaaatag cagggatcaa tatcacctgg tccatcacct
     9361 cggtaacaat gatgctcgct atgacagcag gtctgttttt aagggtatta ttacctgtca
     9421 agccattcaa acacttggct atcctttgga ttatccttcc cttatttcca cctcgtactg
     9481 caatttatcc cggggaagca ttaattaata ttttggatgt tgggcaagga cttgccgttg
     9541 cgatacaaac gcaacatcat gttttactct acgacacggg agattcctac tttcagggga
     9601 gtgatttagg gcaaatggcg ataatcccct ttttcaggtc aattggaata aaaacaattg
     9661 acaaaatagt aataagccat cccgataaag atcataaagg cggattaaat tcccttgaaa
     9721 aagagatgaa agtcaatcaa ttaatagtga atgatcccta tttttataaa cgtggaagta
     9781 attgtcattc atacccgcaa tggcaatggg atggggtgag ttttcgtttt ttacccatca
     9841 gggaatcttt caataaaaaa aataacaatt cttgtatctt acaggtaagt aattcggctg
     9901 gcagggtttt gttaacggga gatattgaaa aagcagctga agagtacctg gtaagaaact
     9961 acggcagtca gctggcatcc gaaattttag ttgtgcctca tcatgggagc aaaacgtctt
    10021 catcctatcg atttttgctg gaagtaaacc cgctttatgc cattgcatcc ttaggatttg
    10081 ataatcgctt caaatttcca cattctaaaa ctttgcaaag catgaaaaca ttaggtatca
    10141 ctttttttag aacagacgaa tgcggaatgg cagcaatcaa actacctgcg tcaggtaaaa
    10201 ttaacaaacc aacctgtatg caggctgtat aaatggattg gctgacccat gttatatcta
    10261 agccttatta gccgattgga accaccagtc actcaatggc gtattattgt aatactttaa
    10321 atgttccgta aaaaattgta acgctttgtc cttttgttta ttgtgtatca acaccaacaa
    10381 gtaagtctga aaaaaaagct ctatctgcgc atcgcttcca cctaattgtg cacactcacc
    10441 aatcaccggc tccatcaaca tcaaggcagt cttgtaatct gcatcggcaa aggcatagat
    10501 accctgacac aaaggcaaaa cgacatcgcc ccatagagca tcagaagaag gtgaacaggc
    10561 gtaaaacttc attcttttca acgactcatc cgcttcgttt ttataacctg acttcaccag
    10621 acaataaata aaatgagcat tcgtaaaccc ggtataatat tccaatggat gggtacttaa
    10681 atggtccaca acctgattta acaacctgtc ttggggcaga cctgccatat ccatgcgcca
    10741 tagcagggaa atagtatcca gctgctcaag cacagtatca ggcaatgccc ctgaaatatg
    10801 ggggtataac tttttaactt cctcttcatt acgatttgcc aaataaaata gcgctaaatg
    10861 ccaggtatta tggcctttca acaaaggcaa aatatcctcc caggtttttt gtaaatctcg
    10921 aagtctattg ataccaccag taatatcact tgttaataaa tgcacatgag ccagggtatg
    10981 atgtgcccaa ggagtaagca aattcattgt gatagcttcc tcagccattt ccttggcttt
    11041 tgaatattgc ccacacaatt ccaaagcaaa ggaatgtatg gctaaaaaat gggactcatc
    11101 ctgattctcg ggagcacact tatcacatac tttaagaaaa tattcagcat taaaagcctg
    11161 tcctgtacag taaaaaagcc attccatgaa tttcagagcc agagtatccc tgggatacct
    11221 ttcaagcaga gtaactaaca agctaatggc attggcataa tctcgcttag accaattcgt
    11281 tactgcttca aaaattaatt tttcacgttc attcgctgat gttaataacc tgaatgccga
    11341 ttgcaaataa tcgctggctt gttcattcgc tacatcttct tgtgcataca aataaaaagc
    11401 cgccgcataa gtttgaatta ataaattgtc agtatgtttt tttgctgaat ccagaatttg
    11461 gattgcgttt tgaccggagc ttagtacctg ttcgtgaaaa taattgatac tatctactgc
    11521 ctctatggag cctgtcgtta cttccaatcc acgttgagta tgcattctcg aaatcctgtc
    11581 aatgaatgaa tcaatgatta aatgatagca caaataataa acaacaagga cggaagcaac
    11641 gctccaatta gatgatagta cggcctgata tcacgatgac agcggtagag ctccaagtac
    11701 tctcgctaaa attgttttcc ctgaccagaa attaatacaa caccagatta cccacttcct
    11761 gctaatcatg ctttatcatt gagttgaatt accatcacct gaattggatg gttagttacg
    11821 ttttcatcgt tatgcaattc gttaggggcg tctttggtga gataatatga cttacctttc
    11881 tttaatttta aatagtgtac tttgccttta ttgtttgtaa ttttcaatgt cccatccgtc
    11941 aatgcgacca aaactcgatc gttatcatgc ctgtgcatgg gcaaaacttg ttttgaagag
    12001 ggatagataa ttgttttcca cacagtcacc ctggcattag ataattgctc aatacgttga
    12061 gttgataatc ctgcagcctg gagcgcactt gtacccagaa caaagatcat aagcaatttg
    12121 aataaattaa tttttttcat catgttagat tactctccat tttcaaatca aataacagtg
    12181 tgtcatctcg cttactcctc aattttcctt taactttccg acacatagct cacaaaattg
    12241 ctataattat agtaggaggt cattgtaagg agtttgaaga tgttaactac atttctagcc
    12301 acagtgattg gctggtattt agtcatagta agcttattct tattattcca taatgagcat
    12361 gcaaaatcag ctatggcaga gattatagct catcgcggat cgcaattgat tatggcgata
    12421 gtaaccttaa ttacaggttt gcttttagtc gtcagtcata atatctgggt aatgggctgg
    12481 cctgttgtta ttactctttt tgcatggtta gtcttacttg gtggtttaat taggttttta
    12541 tgccccgata ccgcagttag aataggccaa tcatttgtcg ataatccaat gagactaaaa
    12601 atagcagctg taatattctt tattattggt ttgttcctgt tatttaatgt ctattatgtt
    12661 tatttgaaat aaaggaagag ttattgaggt actatgactc attctgttga tggggaaagg
    12721 aagcgataaa gtccttgtca attcagcagc aaggaatgct atgatatgat gcttgatatg
    12781 atatgcctac aaaaaaaatg actagagccc aaacctgccc tcatcaaacg accgttattt
    12841 caaatcttaa aataattcca ctcactttgg ggtggttatt aaaatctgct ttgctcatta
    12901 ttataatcgc cgtttttatt ccactatccc ccaaaatgcc agctcccggt ctcgatgcct
    12961 cttgggctgt aggacttaat caagcagttg cccaaggttt ggcttttggt aaagacattt
    13021 tttttacctt aggaccttat tcttcaatct atacaaaatt ctaccatcca gctaccgatt
    13081 tcatgatggt tgggggaagt ttgtatttgg ctttatgtta ctggatagct attatccttt
    13141 taatgcaagg cattaaatgg cgttggagta ttgcctttgc aatacttctc tttagtatga
    13201 tctatgctcg ggattctttg tttttttcct attcattaat cattggctta ctcattcata
    13261 aagaaatttc tggaaaagaa acatccttta aaagcaattt ctgcacttta tttgctttgc
    13321 tttttacgcc attcggttta tttcctctga taaagggctc tcttttaata ttaagtatta
    13381 ttaccgtgct cttatgctct ttatttttga tcctatataa acagcgaaat cttgctgtgc
    13441 tttgtctggc atctccagta gtttccatat tatttttttg gatagcatcc gggcaatcta
    13501 ttttcaatct gccagcttat gtcagtaaca ctatcttgct cgcatcaggt tttactgaag
    13561 ccatggcaat tgaaggagac aataaagaac tgatattttt tggggttacc tcttcttgca
    13621 ttctcttatc aatactcctg caaaaacagc tctcaccatt atccaaagca tttctatttt
    13681 gtatctatct cgtttttctt ttcctctcat ataaaacggg tttcacaaga cattttggcc
    13741 atgccttcat tgctggtacc tctgttttgc tagcctcttt tttgttaccg tattttaatt
    13801 ccaacaacaa agtaatagtc ccattattag ctttatccct ttatacagcg agttatatca
    13861 acgggcacta taccaaaata tctgttcgag ataatttcat ttccacctat accgccgctt
    13921 actatggcct taaaaacaga atccagaata agaattggtt aaaagaaaat ttttcattaa
    13981 cgatgaatta tttgaaagcc cgcgatgatt ttcccagatt accgggtact accgatattt
    14041 attcttacaa tcaaacttca ttaatcgcct cgggaatgac atggtcgcca aggccaatct
    14101 ttcagagtta ttccgttttt actgcaaaaa tggcagaaat caatacgaat taccttttaa
    14161 gtgagaacaa accggataac atccttttta aagtagaacc aattgacaat agaattcctt
    14221 caatggaaga cggaatgagt tggccgatac tgattaatca ttaccaacca gtacggttag
    14281 aaaatgattt tttattttta agaaaaaaat tagtcaacaa acctgtaaca tctttagtgc
    14341 cattaaaaac agaaattcat acttttggag aacaagttaa aatacctgat accgataaac
    14401 ctctttttgt cgaaatagaa attcaaccaa cgctctgggg gaggttagcg actacatttt
    14461 ttaagcctca ccagcttgaa gccaatttgg agttaaaaaa tggtacccaa agacaataca
    14521 ggctcatttc caatatggca aaatccggtt ttctattatc gcctttaata gaaaacagcg
    14581 ctgaatttgg attgttattt agccagaata attacctggc aggtaaaaaa gttatttctt
    14641 tttccattaa ctcaaagcaa gtgccttcct cgcactggca aacgaaatat actattcatt
    14701 ataaaatttt tgccagcaat aattaaaaca ctatggatga ggcaatcaaa atagtatacc
    14761 aagctcttct ttgttagttt ttgtttaaat tccaaacaaa tatcttccca aaaggaaaat
    14821 taacaagtaa aattatgtac taaaataatt catatagttt tatatatgcc tttagggttc
    14881 tttttatgtc caaaaaatat cgaactccca tccgattgtc agacaagatg aaaactctta
    14941 aaaagaatct taaggatcaa cttgcacaat ccaaatcgtt aggtgagcaa attcatgccg
    15001 gaactacggc agcatcaaca ataacaacgg gagttagtca tgtgtttaat gtcattgact
    15061 cctttttaag cgcctatcga caaattccac tggtaggcgg aatactgcag ctagtggcca
    15121 caattgccaa atcaatttct attgtgagtg atccagaaaa accgatagct gaaaaaataa
    15181 tatcgctgat tctaattgct gccattatcg ccctttctat tacagcgatc actctcggtg
    15241 gattggctgc cgcaatcatc gggacagtaa ttgcttctac tgtaaccatc atggaaggat
    15301 tgggattttt tgggaaaatt gctgaaaaat ttcaattggc aaattcctac aaaatgaaaa
    15361 aagaatttat agatcttgta gcgaaacgta tagagccaaa caatgataaa tataatgact
    15421 tattagaaat tcgtgccata gaactccaac atgaacttga gaaatcttat cccaatcaaa
    15481 ttgaacagca acaattaaaa gaagagttga atttcattaa tgcggtactt aggaaaaaaa
    15541 atatcacccc gggcaacaac aaagagaatg acgcttacaa actaaaccaa ctctatgcta
    15601 aacgcgctga gcaattaaaa acccttgtag ataaaattgc tttaatcaat ccagctggaa
    15661 agagagctga gcaaaacaaa ctgttaagcg aaatcaaatc attacaaaaa gaaataacca
    15721 aaaccgacga tagcatagaa gaaatcatag ccccgcttgc taaaatagaa cgagacaatt
    15781 tgaaatctac cattaatttg ccattaagtt acagtacctt tgctatagca gcggccagta
    15841 ctttaatatc gatagccggc ttattggtgg tggctggcgt gattgccgca cctccgatta
    15901 tggtccctat catgtttgca gtaggtagtg ttatggcaat cattggttta atcaagtgga
    15961 ccgctgaaaa aatggctgaa atagaggacg ctcaattaaa agcagaagaa actgcgaaac
    16021 acgaagatgc agttttagat gaagcattaa acgtttatga gcaacaatta aatcttgagt
    16081 tagtcagtcc ttcaaaccct gcgagacagg tgaaaaatat attaacagtt cacgataaag
    16141 tgcctgcttt gtcgccaact gaaactactg taagctcatt agattccaga tttactttat
    16201 ttggctcatc ttctcgagag cacacaaaaa tagcagaaca gaaaaccact gatacaacta
    16261 atgaacaagc acctacaact tttgattcca tatccaaacc aactcgttaa caaatataac
    16321 aactttttat aactttgtta taattgattt acaaatagtc agtttatgcg taatacctga
    16381 tttgtgaata taaaaattaa tgaaatcaag agaaaaattc atggccagaa ataaaagact
    16441 cgatcgtttt cataatgctc aaaatagtaa tacttgtggc tacgatcaag cattcagcga
    16501 aatacaagac ggaagaaaaa gaagccattg gatatggtat atttttcctc aattggaaaa
    16561 tttaggtaaa agcccatcgg ccaaactata tggaattaaa gattttaatg aggcatgtga
    16621 ttatttaaaa gatcctgtac tgtttaagcg ctattttgag ttaataaaac tcatagagga
    16681 aaaattaaag aacactcctc tacctcatct gatgggtggt tttcctgatg atcgtaaatt
    16741 catctcaagt ttgaccttgt tccaagccac ttccaattac ctgagttcaa aagatacgga
    16801 ttctcaaaac aattatcatg agctttatga gacttgtaat agagttttta aacaaattgc
    16861 tccacagggt tattctccat gtcaacaaac tgagttattg gtcaaacatc aatcaaattc
    16921 ccctgtgaaa aagacaacag aaaaatcaag tcaacctcca caagtgacta tcttttcacc
    16981 caaacggtcc aaaccgaata ctgctcaccc tatccttaag gatcttgctc aatatataaa
    17041 caaaagggga aatgaatgga gttatcatta taattttcta ggcctagtgg ctttcgttta
    17101 tttcatacag gatttaattc ttggaactga tcatttcaat cataaagccc gtgaggtcaa
    17161 attaactgcg gcaagcaaat tacagaaaat tcttgatccc caatgccatg aacgatgtaa
    17221 tctaaatcca tcagaaagaa aggcgctttc cgatggtcgc ttgggggaga ttattaataa
    17281 acacggggga ttagataaaa tcctgacaga ggctcctagg aaacctccaa aaactggcca
    17341 cgatcaggaa atcaatatta gtcccttttc ataaaaccat tggatttcaa gtctttgcac
    17401 caagaaacat cacattataa acccaatggc ttttacaatt ttttaatctc tacgaattca
    17461 ctacaaacca catcaattat tattcaaaag actcccatag agcgacatgt gcaccagtcg
    17521 gatcgactat aatagcaaaa cgtcccattt caccagcctg cgttattcct ttgacttcct
    17581 gagcgccatg ttgtttggct ttttttaaag cctctaatac atcctggaca taaatataag
    17641 ccatccagtg aggaggtata tgactttgtt gatctttggg aatttgccat ataccagcca
    17701 tttccttatc attgcattga ataagagtgt aggtcatttc ctttgattca atctctctaa
    17761 attgccaacc aaagaccttg ccataaaaat cctttgcctt tttaacatca gaagtggcta
    17821 attcattcca acaaaactct ccaaacgcag cttgagtcat aagaatctcc tttaatttgt
    17881 tttttagaac aacttgattg gtattcggat ttatgcaaaa caccacaccc aaggcaaaaa
    17941 atatttttaa taaggggatc tagataaact aaatgaccga attaaaaact tttttcacaa
    18001 agagattggg attttcgtaa gtccttatac tgttagcaag cgatttcctg attaccttca
    18061 ttacttgaca acgaatacaa cttaatgaat agttcacaca gaatagtaga ccaatgttat
    18121 aaatactgcc attaccagga ttaaatattt ttaacccaat ctaataatta cattatgagt
    18181 caaaacatca ttctaaaccg atgcgaatgg gtagggcaaa acaaacccca ttatgaacta
    18241 taccatgata ctgaatgggg aattcctgtt catgatgatg aaaaacattt cgagatgctc
    18301 attctggaag gtgctcaggc agggttaaat tgggaaacta tattaaaaaa gcgcgatgga
    18361 taccggagag ctttcaagca atttaaccca caagccgtag cccaaatgac tgatgatgaa
    18421 ttgaatgctt tgttaattaa tcctgaaatt atccgcaacc ggcttaaaat cttttctacc
    18481 agaaaaaatg cgaaggtttt tctatctatt caacaagaat atggatcgtt tgattcttac
    18541 gtttggcaat ttgttaatgg agcacctatc ataaatcgtc ctgaaagcat acgagcgata
    18601 ccagcaacca gcaaagaatc agatgcctta tccaaagatc tgaaaaaaag aggaatgagc
    18661 tttgttggct caacaatcat ctacgcttac atgcaagcca ttggtatggt aaatgatcac
    18721 atcgtaacct gttttcgagc caaataaaga aatcactcga actcctgaag gtgactcata
    18781 ttctccataa gctttttagc gaaatcattt aaaccggatt atctgtcgtg tcctggccta
    18841 accataaatt ggcaatgcag tttcatgttt tgttaggcca aggaatgata gtttgaccac
    18901 cctaatcagg ttctcaagct gactgtaatg taagggccac ctacagaaaa attactttgt
    18961 gtgtgtttgg cacttgatgt cgacaaagag ccagtgtcca aggcaataac attggtattt
    19021 gtttcataac tggataaagg attaaaatac aaagctccca tataccctgc ttcaaaattc
    19081 aaatcccaat tttgtttcaa tgagtaaaga taacttaacc caattttggc atccagagct
    19141 ggaaccagtt gattgacact gggattagca agtccttcac gatttacaaa gattccagcg
    19201 ataatcaatt gctgagaggt tcctgtgaat tggtattgag ctggttgcat tctgccgtac
    19261 aagactgagc cagcgagttg tccgaccaaa ttgaacccat gagaaatatt ataactatta
    19321 ttgaatccta gtctcggtcc aacacctttg taagtggatg tattatttaa tgacagtata
    19381 gggagtgaaa agccccgaaa agttgatatc aactcttcct ttatccaggc actgctcaac
    19441 cctgtaaaaa gccttgtctg taatttggaa ccaaagttaa taaatttccc aacatcgaga
    19501 ctgactgcat cataactaaa atcaagactg gcatgtgcaa gcttcaataa agccactcct
    19561 gtaatttggc cagaaggcgt aggaggagta ccggtctgag aaaaaggaga gatccattgc
    19621 gatgagggat tcacaaagac attatcagta tcattggtac gcagatgaga ccagtttaat
    19681 tgcaaatcgc ttccagaatt ggcaaataca taacgtcctc ccaaattaaa cccaacttga
    19741 tagtctggat taaaagtaac aacctgccaa ttaggcgatg gtattggcag tactgtcgta
    19801 atcacccccc aacctaaatt atccgctccc ggttgcagat aaaaagctgt cgcagaaaat
    19861 tcatatccag gtttcaaatt aggaatgaag actcggaaca ggtcgctatt ttcaactggc
    19921 cctgtatcac cggcataatt gcatacacca aataacagta agctaattcc cataagtaat
    19981 tgatgatatt tcttcatatt taatgaatcc tccctaatgc atgatttcta tcaatcagga
    20041 cttacgaaaa accctaatct ctt
//
LOCUS       DACTJE010000045        20047 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.045, whole genome shotgun sequence.
ACCESSION   DACTJE010000045 DACTJE010000000
VERSION     DACTJE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20047
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.045"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..279)
                     /locus_tag="I9760_001261"
     CDS             complement(<1..279)
                     /locus_tag="I9760_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043037.1"
                     /translation="MKQLILHPTDISQWHALVNEAQAATRLILNENTESYLVFLLMRF
                     SQGTKLIESVVALDFLESMRKPRQLQVELLRDVGDKSLLFCGLFPGIAK"
     gene            complement(547..1506)
                     /locus_tag="I9760_001262"
     CDS             complement(547..1506)
                     /locus_tag="I9760_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAT5043038.1"
                     /translation="MSFKGFKVVMLILLSTQSYASKLASSIVCDSTIENPCIVQDSKT
                     QFSPVIRYREVASIADVYGGNITGINKFHLSGSEQPSEKGWEAIAESISRKMGAETKK
                     VIVLDLRQESHGYLNGRAITLVSAYNWINLGKSNSQSTLDQENWLAGLRSRKIVNGVL
                     TVPQYVAKQYSQGKSMVVSTVKNEEYYVYKKGFDYYRIFISDHRAPLDSEVDALVALI
                     KNNPEDTWYHVHCRGGKGRTTTVFAMFDMLKNADKVSFEEIIARQASIPPFYNLMVTN
                     REIPELTPYYEQRLQFLIHFYEFARQSLMGYSGTWSEWKKLNI"
     gene            1684..2694
                     /locus_tag="I9760_001263"
     CDS             1684..2694
                     /locus_tag="I9760_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAT5043039.1"
                     /translation="MKYRKLGKVGPDVSALGFGCMGLSEFYGKPARLDDAIKIIRNAH
                     DQYQVNFFDTADIYGKGKNEELVGKAIKPFRNQIILATKCGVVRTGSNDEMSVNNSSA
                     YIRTACEKSLKRLNTDHIDLYYLHRYDHQTPIEEVMEIMHDLIDEGKIGYIGLSETDA
                     DTIRAAYTAVKDKLVAVQTEYSLRVRAPATAVLGTCRELGICFVAYCPIARGFLSGKI
                     KKPNLLRTNGFDFRTNIPQFQPENFDQNLGLVNELETIGEKTGYTPAQLSLAWLLAQG
                     EDIIPIPGTSNPQHLAENMRSIDIFLTPEQMKDLENAYKNNPVAGKRLSKELMATFHL
                     KE"
     gene            2802..3614
                     /locus_tag="I9760_001264"
     CDS             2802..3614
                     /locus_tag="I9760_001264"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HAT5043040.1"
                     /translation="MDYYFTKGKPQLVNMKTPVYLVTQFQQLMDLMQNQYQVSCLELM
                     QRSGKAACDFLVYRWPKVKKISIFCGRGDNGGQGYVLAQQAKKMGMIPTVWQVGHQMS
                     MSKPPQMHEEVWYEMNSCHQQGILLHTYSPDIDLGDPELIVDALFGVGLYGHVRPEIA
                     SLLQRLQQFTVPILAIEVPTGINASTGEIAGNALAATATITFLCMKLGLLINDGKIYS
                     GEIAFDDLLAPEAIYQQVKGIEKSSLLDSSTCFSKKIWYRNKTQKGWQLSIN"
     gene            3726..5552
                     /gene="typA"
                     /locus_tag="I9760_001265"
     CDS             3726..5552
                     /gene="typA"
                     /locus_tag="I9760_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAT5043041.1"
                     /translation="MIEKIRNIAIIAHVDHGKTTLVDKLLQQTGTLNERAPKVERVMD
                     SNALEKERGITILAKNTCVYWKGYQINIVDTPGHADFGGEVERILSMVDSVLLLVDAV
                     DGPMPQTRFVTQKAFARGLNPIVVINKIDRPGARPDWVMDQVFDLFDNLGANDTQLDF
                     PVVYTSALNGYAKLNLEDNATDMSALLQTIVDKVTPPCVDANGPFQMQISSLDYSSYV
                     GTIGIGRITRGQIKAKSPVKIIDKDGNMRSGRLLQLLGFKGLERVEVEEASAGDIIAI
                     TGIESLNISDTICDPNLVEALPALLVDEPTISMTFQVNDSPLAGQEGKYVTSRKIRER
                     LNTELLHNVALRVEDCDDPDKFRVSGRGELHLSILIETMRREGYELAISKPEVIIREV
                     EGEQQEPYEHLTVDVEENHQGTIMEKLGERRGEMQNMVPDGKGRVRLDYIIPTRGLIG
                     FHNEFLSCTSGTGLMYHVFDHYGPLIKGRIGKRLNGVMIANCPGTARAFALFNLQERG
                     RLFLEPQSVCYEGMIVGIHARDNDLVVNVTKEKQLTNIRASGSDENIILTPPVKLSLE
                     QALEFIDDDELVEVTPLSIRLRKKFLKEHDRKKASRTATSEE"
     gene            5559..6446
                     /locus_tag="I9760_001266"
     CDS             5559..6446
                     /locus_tag="I9760_001266"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAT5043042.1"
                     /translation="MKQKFKRAILYARQHRANQEVNESLHRLVDFLSTQDIEIFQDSD
                     TAASFELKAPVLPREKMGAKHDLIIVVGGDGSLLSASRMAIKVNTPVIGINRGRLGFL
                     TDILPQDIESHLGPVLNGQYNEEERFLLHTKIYDKENSYFEGDALNDVVLGRGSETHL
                     IEFDVYINQQLVSHYRSDGMILSTPTGSTAYALSAGGPIMHPQLNAIVLVPMFSHSLS
                     SRPLVIDGEAEIELYISKSNETDLRISCDGHESRVVKPGQKVAVKKNGNRLRLLHPLD
                     YHYYDTLRSKLGWESKHQG"
     gene            6450..8117
                     /gene="recN"
                     /locus_tag="I9760_001267"
     CDS             6450..8117
                     /gene="recN"
                     /locus_tag="I9760_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAT5043043.1"
                     /translation="MLTTLRIEHFAIVKQLELDFTQGMTTFTGETGAGKSIMIDALLL
                     ALGEKADPSVVRPGEEKCDITAGFCFDESSELAQWLAEHDISCHDGEIYLRRIIYAEG
                     RSKSYINGQPFPLQKIKELSEKLVHIHGQHQHQTLMQHATHRQQLDRYANNHLLLNDV
                     NTLYKQHQQIVQEINALQSQEQQADRIKLLQFQIDELQALDIQDGELEALHQEHQILH
                     HAREYLQLSQQINELLNSDDQPNICSGLNQIFQLIQQLPQEQAQIKNTAELINSALIQ
                     CEEAITEMQQFADKIQLDPERLQEVEARISSIHQLARKYHLDSKSLQEHEKTLQNELE
                     SLQNVESKLAHLHLQLAQINKVYESAALKLSESRKIHAKKLAQEITASIQQLGMPKGW
                     IEIEITPLEKMQAHGLDKVEYKVCTNPGMEPDSLNKIASGGELSRISLAIQMITAQKG
                     STPTLLFDEVDVGIGGSTAALVGKMLRKLGERLQVFCVTHQPQVAASAHHHFLVTKHS
                     NNNQTFTHISLLKASDKIAEIARMMGGLTITEQTLSHAKELISLSNE"
     gene            8284..8490
                     /locus_tag="I9760_001268"
     CDS             8284..8490
                     /locus_tag="I9760_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAT5043044.1"
                     /translation="MSEKIRGTVKWFNESKGFGFLESGGKDYFVHFSAIQGSGFKTLA
                     EGATVMFKACNGQKGPQAEEVEVV"
     gene            complement(8829..10562)
                     /gene="ceg34"
                     /locus_tag="I9760_001269"
     CDS             complement(8829..10562)
                     /gene="ceg34"
                     /locus_tag="I9760_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg34"
                     /protein_id="HAT5043045.1"
                     /translation="MLMIMARVIVTYSPEVGGSSVLSLKESFTHVGSEVIDGDYRKMM
                     EDIPKEEFDKLYSTKEGRQRLFAHAKAKAMELLKNVDVLALSGNNSMIDPELFNQTRD
                     PDQNYDFSRTIAELALVHVATEKGMPILGVCGGHQVIAVYGGGEICDLNSDKLNTQKV
                     MNYDAIKVHKGSLIAQIIGGKQVQKEMDTARKAFNERMNQYNRLSEKLNALEKLNLVQ
                     EAGKLDALGKLEELDKSEVQLLLKLTGLNIEEELFDLRELVGEIGETNQLKTELQNIK
                     TSLNGLEDSHPLTSPHYETEFFGAHEQVVSKLAAGFQHTGTASDNESNEAAESEFGVP
                     IITTQFHPEVGAKGLPEAHFFYLRTEAEQEMNLNIFRYLDKAGDAYNKKKNLMVELKQ
                     INPKNQDVLLQPSQIKKTSKERQQEREKQGNKSSSKRQTESYFFESILSKIGSYLSAI
                     GEFIREGIGKFLRDILSSMFTYDSVSRLKKKEQKQSKSEHSKIDLIDSKSNSYSTVFH
                     DMKVKSPSEILRENPAKLWAEKDDSHEKTITNQTDMDNSNKVRITTELVQEEVGQEFD
                     ENHTSLYRNNL"
     gene            11001..11885
                     /locus_tag="I9760_001270"
     CDS             11001..11885
                     /locus_tag="I9760_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043046.1"
                     /translation="MVPPEGFCFNYETSTSNSFQSFMSIKDISTKALAEFTAMVLQLE
                     KEEIKVLTLSQRQDLPDAVFPNNWFSTHIDARGNNILIIYPLLAKNRQAEVNIKGLME
                     VLNQAQFEVHEIIDLRNDKDEILEGTGSLVLDRENHLLYASLSPRTSLNLVNKVADIL
                     GYTPIVFNSVDRQNKPVYHTNVIMSLSQCYAIICLDSIHDTQQKAMLSESFKETGKTM
                     IDISWNQAQHMCGNVLELYNKKGESLLILSEQAKRHFTHEQLRIIQQHSRLISTDIPT
                     IEAIGGGSARCMMAEIFY"
     gene            12033..13289
                     /locus_tag="I9760_001271"
     CDS             12033..13289
                     /locus_tag="I9760_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2828 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043047.1"
                     /translation="MKISELKKLVAELNKEVSPKVTCTNIINLAGNLSEIIEYFEQDE
                     HVGPELIYRIEAVICEFWKLVSLTLPYEEWQSSIQVAPWLILQQSLSKAGLLPTDFHH
                     PILYQRLKERYESFGHSELGVDQLLPLLIRCSRMTGYANKDPQSLDTYPHSPLNKQIE
                     ARRPQELAKLKDILCLLRAIFYLIHHCCTIEQLTLIPYLIYFRNPTTDEERRSELAIF
                     NWLTQKPADCLEFFKTNEDYIDTRSFRQISELAPLRPFIPTARSDFIKITNREHWIYP
                     FIQSRTNTSRSEYDLLNDAVNWLDTDFATEKDKSYHAALEFAHTVKKQANILTQREMK
                     IVHSALYVFCLDKYIKHRKADPRPRCTPFSLSGETKCQAAEKKQQEILGKPTKFGFFE
                     NLALNEGRLKTLTKTFEMPPYPLLRN"
     gene            complement(13356..20033)
                     /gene="sidH"
                     /locus_tag="I9760_001272"
     CDS             complement(13356..20033)
                     /gene="sidH"
                     /locus_tag="I9760_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidH"
                     /protein_id="HAT5043048.1"
                     /translation="MKRTIETYIIYLKEDLKIADTCKTIKDGLLKSITDKTHFSEELA
                     TYFERDNPNAPFKVNTTDPTQVAVLKKLLNALENAEKSFRAIENIDISRDRYTAMIAK
                     DAVMVSYKAVHEIYAALQLINHSNSDIQDIVGPHIQKLLPQMALASKALGNFAPEHPE
                     ESAGAVLAGVVNMLPTEKPTESESLGKLSNLIFELPHYFEELQKLIATGASGIATKSI
                     TSAEDYQSAMIKKANETKYYFEQLSSKSGLLAIPSYLSIVKRLIAHSTDLVNAGAPLT
                     KQAYLDAVAKLEDIKHNILPQLISELEMVEESMGLKPGLLTDPALEQMNKYYTQLAEQ
                     VDNIAKAAGVLDTVSDYSDSIGGKIVRFLAGDSKKLDVGPKLTPAPDLGVLMDDVFIQ
                     KRRSNQESRLNEARLSSEDKSVLAAANRFFDKIGSYNSIHKAWSKWSLANISQSEKDA
                     LIKEYKQFQPHFAALYPDIDKLVVDALTQPTGSNIVSRLYSSDYKQLWSSDHFKQVLS
                     CKDSVLSSIQQSLAQSEFKAKLIEKTMSHSEETAYSMNNKTTNLTTRVQPFEPLKFTL
                     EDDKPVEYYHKRVIAASNQILELERAQKGVAEFFNYIQKKYPHENPSFDSLDESDKEF
                     LRKAYKTFQPQLLALKHDDINTRLVSSLTSSKPTDPPLRLTDLVSLKSGINDYLNEKI
                     SDLNQDKTTLLDKEEEAREEQYAKNPLVAKGAELEKQTLFGQMSKLKLSKSVDDFFNK
                     KFQTYLKDNLSPEVWKQLSSNGETLDFDKIPYLEFHKDSPEVAMYKQLINSMHYMKNG
                     LEKLESLNDYGDPNNIYHRTRFVMTTFNALVMNICFSKYYVMEAGNNPGLKAIVQEGL
                     DLLKPLEGMPLIGDYLKTTEKQEPPKQNIITAWKKQQAVVESGLSKGPKPKTDQQLIS
                     EQLGKIQEAIDNFDGDLEVSDSAREKIKTQIGEFAKGISGLSFGPGSVKKILAALTKL
                     ETQLSNLDKESPEVTLGKLKDIHSELNAQFRAAAEYTEYHSGQKFGSYSNNISTIVSN
                     FCNGLVSNLPLKQEPAPKVKAPEKPVTPVITGTTNPHEVVFGTKHEEFNSVYQPYLLL
                     KRITDEFRDQNNPYKPSFDELKEEAVSYYDKIQPLLESVDPKFDKNFIAKHKESSTLL
                     KAIDEVMSMRQRINNPESSFAKLKDLHLEGDFEKEENKEKFRQLYKEIQPYLHKIDST
                     YDQTQFLEGLKTAKDFSGALQRIMNEENALQQSTSLKDTSYLQLIAESLYQIPVKLNK
                     LKAEPDTPEPSKEEIDANVKAFVEGLNGLSFGPGSVKKILSTAAKLQMQLSDIGKEGR
                     ELTMGRLKEIQAEFGTILMAAADNAEFHLGLKPGTYSRTVSERFEKFYSSLIVNLPLE
                     KDQTGLELLIDTTSTQKRLAREMERLESVKEDTSAIDTKKSIFGTEHEQFSTLYQPYA
                     SLRHIAKDIEDGVHMNLYERTLEELKEEASNEYKKIQPYLAKINPEFTEDYISKTDGE
                     YSLLHAIDRVFEERHKINKPSSPFDKLRDLYLDGDFEKEENKEQFLQLYAELQPHLIK
                     INYQYDLAYFLRELQTPEDFKAATERIINDESKLQELITGLDDTKRLKVKLCEERIGY
                     FIDLLKKQELEVGPEKIQAFKEKIFFNYIHANVNSALDAKIGSHAEQFLQFIEKDFLD
                     KKNEILEKITIDQDMEEEIAKAIDRIAPDIINNKIESFKKLLFDSYIQSDIKNSLHNE
                     LGIYTSLFIDKISPEIHLHQSEILGNVAFDSKMGAEIGSKINAITPGILLNNNPLKEA
                     YVDLNNTLKEINTLLDEENKKTRDNPCRNEKIAKLTSLKDRLSDLDSIPKENTVEFLK
                     KMQEETRSSLKSLESNDALINIYDVLNSLKETIENGSDLPEIKKDKLQMISDVQNILS
                     NFDKNPAERLNLAVQILNDSNPEVLSKTKGNFLIGEAFKGQVFTNYINTKISEQLNNE
                     LGPYGKVFLKQIMPDFIAKKSEIIKEIAIDNMETGLETQFKIHAPAIFEKNKELKAAY
                     EQLNVHLKEVQSLIEAEEKKPKGNPCREEKIAALRSHQSQLMNTQRIPDHETLRFLQE
                     QNKSAKSFMGKLEKYDTMISVYDSLTEIREHVSNHKSLSKEIKDEKIQEISKMEDMLK
                     TTSKEPSIRLAEVKAHGLSDQCKNVLLKNSDNFLVSFFKTLFSKLFNIKNENETLVSS
                     FKQRLQNIKGPEPVATPMETPENEAPLVNANITRF"
BASE COUNT         6107 a         3916 c         3643 g         6381 t
ORIGIN      
        1 tttagcgata cctggaaata agccacaaaa caacaagctt ttgtccccca catcccttaa
       61 taattcgact tgtaactggc gaggtttgcg catggattca agaaaatcaa gtgcaacaac
      121 ggattcgata agctttgtac cttgagaaaa gcgcatcagc aaaaaaacca aataactttc
      181 cgtgttttca ttcaatatca gtctggtagc tgcttgcgct tcattaacca gcgcatgcca
      241 ttgactaata tcagtgggat gtaatattag ttgtttcata aatacccccc atatttacct
      301 cttactatta gtctagcaaa caaataccaa ttgttttcta tgactaatat aataattcgt
      361 aatgcatttt tatgattaaa tcaagatttt cttatcttgg agcagataaa tcccaagaaa
      421 accaacactt aatttaagat tgatatggta tttgccgaaa acaattgagt gccaagcctt
      481 catgaaattc gcttcatttc aaatgaaggc tttttaatat gatcttcttg caaatggcac
      541 tagatgctag atattgagct ttttccattc tgaccaggta cctgaatatc ccataaggga
      601 ttggcgagca aattcataaa aatgaatcaa aaattgcagt ctttgctcat aataaggagt
      661 taactcaggg atttcacgat tcgtcaccat caaattataa aaaggtggaa tggaggcttg
      721 gcgtgcgatg atttcttcaa agctaacctt gtctgcattc tttaacatat caaacatggc
      781 aaaaacagtc gtcgtacggc ctttcccgcc acggcagtgg acatgatacc atgtgtcttc
      841 cggattattt tttatcagtg cgacaagcgc atccacttca gaatctaaag gagcccggtg
      901 atcggatatg aatatgcgat aataatcaaa tcctttttta tatacataat attcttcatt
      961 ttttacagtg ctcactacca tcgatttgcc ttgagaatat tgtttggcta catattgggg
     1021 cacagttaaa acaccgttaa caatttttct ggatcttaag ccggctaacc agttttcctg
     1081 gtccaaagta ctctgactat tcgactttcc caaatttatc cagttatagg cgctaactaa
     1141 agtaatcgct ctcccattta gataaccatg actctcctgg cgaagatcga gcactatgac
     1201 tttttttgtt tcagccccca tttttctgga aatagactcg gcaatagcct cccaaccctt
     1261 ttcgcttggt tgctcgcttc ctgacaaatg aaatttatta atccctgtga tattgccacc
     1321 gtaaacatcc gcaattgatg caacctctct atatctaatg acgggtgaaa attgggtttt
     1381 actatcctga acaatacatg gattttcaat agttgaatca caaacaatgg aggatgccaa
     1441 tttagaggca taactttgag ttgataataa tatcagcatt accactttaa atcctttaaa
     1501 gctcataata aatccttttt aaatccctaa agatgaaccg cagcattcta tgtaaaagat
     1561 ttttattatt caagacgcgt taccatttca gaaataaaat cacaatcatt aatatgttac
     1621 tgccaaataa ttctatactt tattcaactg tccaaataaa accatgtcag ttgaggataa
     1681 accatgaaat atcgcaaatt gggaaaggtg gggcctgatg tctcagcatt gggctttggg
     1741 tgcatgggat tatcagagtt ttatggcaaa cccgctcgcc ttgatgatgc aatcaaaatt
     1801 attcgtaatg cgcatgacca ataccaagtt aatttctttg atacagctga tatttatgga
     1861 aaaggtaaaa atgaagaatt agttggtaaa gcgataaaac cctttcgtaa ccagattata
     1921 cttgcaacga aatgcggagt agtaaggaca ggctctaatg atgaaatgag tgtcaataat
     1981 tcatctgctt atatcagaac agcctgtgag aaaagcctaa aacgcttaaa tactgaccac
     2041 attgatctct actatttgca tcgttatgac catcaaacac caattgaaga agtgatggag
     2101 ataatgcatg accttattga tgaaggcaaa attggttata ttggtttatc tgaaactgat
     2161 gctgatacca ttcgggcagc ttatacagca gtcaaggaca aactggttgc tgttcaaact
     2221 gaatattcac ttcgagttcg agctccggcg acagcagttt tgggcacctg ccgtgaattg
     2281 ggtatttgtt ttgttgccta ctgccccata gcacgtggtt tcctcagtgg aaaaattaag
     2341 aagcccaatc ttttaaggac aaatggcttt gatttcagaa ctaatattcc gcaattccaa
     2401 cctgaaaact ttgatcaaaa tcttggctta gtcaatgaac ttgagaccat aggagaaaaa
     2461 acaggctaca ccccggcaca attatcatta gcttggctat tggctcaagg agaagacatt
     2521 atccccattc ccggaacctc gaatccacaa cacttagcag aaaatatgcg ctcaattgat
     2581 atttttctaa ctcctgaaca aatgaaagat ttggaaaatg cttacaaaaa taatcctgtt
     2641 gccgggaaac gattatctaa ggaactgatg gcaacctttc atttaaaaga ataagctgta
     2701 tgtgaatagc ataggagaat gacgctttct taaccgcaaa tcaggaaact ttccaacatt
     2761 ctaaaaacca tgtttacaaa agttagaaaa gattttttta tatggactat tattttacta
     2821 aaggaaaacc acagctggtc aacatgaaaa cgcctgttta tcttgttaca caatttcaac
     2881 aactgatgga cttaatgcaa aaccaatatc aagtttcttg tctcgaactt atgcagcgtt
     2941 cgggcaaagc agcctgcgac tttttggtgt atcgttggcc gaaagttaaa aaaatcagta
     3001 ttttttgtgg tcgtggtgat aacggaggac aaggctatgt tttagctcag caggcaaaaa
     3061 aaatggggat gatcccaaca gtttggcaag taggacacca gatgagtatg tcaaaaccac
     3121 ctcaaatgca tgaagaagta tggtacgaaa tgaattcatg ccatcagcaa ggtattcttc
     3181 tgcatactta ttcgcctgac attgatttag gcgatcctga attgattgtc gatgcccttt
     3241 ttggcgtagg gttgtatggt catgttcgcc cggaaatcgc ttcgcttctt caacgcctcc
     3301 aacagttcac tgtacccatc ctggctatcg aagtgcctac aggcatcaat gccagcacag
     3361 gtgaaatagc aggtaatgcc ttggctgcaa ccgccaccat taccttcttg tgtatgaaat
     3421 taggcttatt aataaatgat ggaaaaattt atagtggaga aatagcattc gatgatttac
     3481 tggcacctga agcaatttat caacaagtca aaggcataga aaagtcaagc ttgcttgata
     3541 gttctacttg cttctccaaa aaaatttggt atcgcaataa aactcaaaaa ggctggcagt
     3601 tgagcatcaa ttaaagagta gatattcttt aatgagcgat tagtgtataa tgttaaatct
     3661 atgaacaatt gtcagttatt atcttaaatt attaaatctc gctattcccc aactaaaggt
     3721 tttttatgat tgaaaaaatt cgcaatattg ccattattgc ccacgttgac catggcaaaa
     3781 ctactcttgt tgacaagctc ttgcaacaaa ctggaacgct caatgagaga gctccaaaag
     3841 ttgaacgcgt gatggactcc aatgcacttg aaaaggaacg tggaattact atcttagcca
     3901 aaaacacttg cgtttattgg aaaggttatc aaattaatat tgtagatacc ccagggcatg
     3961 cggattttgg cggcgaagtg gaacgtattc tttctatggt tgacagcgtt ttattgctgg
     4021 tcgatgcagt tgacggcccc atgccacaaa cccgctttgt cacccagaaa gcatttgccc
     4081 gtggcttaaa ccctatagtc gtgatcaata aaattgaccg tccaggagca agacctgatt
     4141 gggtcatgga tcaggtgttc gacttgttcg ataacttagg cgccaatgac acccaactag
     4201 acttccctgt agtttatacc tccgctttaa atggctatgc caaacttaat ttggaagaca
     4261 atgcaacaga tatgtctgcc ctactgcaaa caatcgtaga taaagtcaca ccaccttgtg
     4321 tcgatgccaa tggtccgttt caaatgcaaa tcagctctct cgattattct tcttatgttg
     4381 gcaccatagg cattggccgg attacacgcg ggcaaattaa ggcaaaatct cctgtaaaga
     4441 ttattgataa agacggtaat atgcgcagcg gacgactgct ccagttactg ggattcaaag
     4501 ggctggaacg cgttgaagtc gaggaagcca gtgccggtga tattatcgcg ataaccggaa
     4561 ttgagagcct gaatatatcc gacacgattt gcgaccccaa cctggtggag gcattgccag
     4621 cacttttagt agatgaacct acaatcagca tgacttttca ggttaatgac tcgcctctgg
     4681 ccggccaaga aggtaaatac gtcacatcaa gaaaaattcg tgaacgctta aatactgaat
     4741 tactgcataa tgtggcgctt cgcgtagagg actgcgatga tcctgataaa ttcagagttt
     4801 ctggtcgcgg cgaattacac ttgtcgattc ttatcgaaac gatgcgccgt gaaggctatg
     4861 aattggcaat aagcaaaccg gaagtgatta ttcgcgaggt agaaggtgaa caacaggagc
     4921 cttatgaaca cctgacagtt gatgttgagg aaaaccatca aggtaccatt atggaaaaat
     4981 tgggtgaacg ccgaggcgaa atgcaaaata tggtgcctga tggcaaaggc agagtacgtt
     5041 tggattacat catcccgact cgcggattaa tcggttttca caatgaattt ttatcctgca
     5101 cttccggtac cggattaatg tatcatgtct ttgatcacta cggtccgttg attaaaggac
     5161 gcatagggaa gcgtctcaat ggcgtcatga tagctaattg tcctggtact gcccgggctt
     5221 ttgccttgtt caatttacaa gaacgtggaa gattattttt agagcctcaa tcagtctgct
     5281 atgaaggaat gattgtaggt atccatgcca gagataacga cctggttgtt aatgtcacca
     5341 aagaaaagca attaacaaac atcagggcat caggttcgga tgaaaatatt atcctgactc
     5401 ctccggtcaa attatctctt gagcaagcgc tggaatttat cgatgatgat gagttagttg
     5461 aggttacccc gttatctatt cgtttacgta aaaaatttct caaggaacac gatagaaaaa
     5521 aagcctctcg taccgccaca agtgaagaat aacacattat gaaacaaaaa tttaaacgag
     5581 ccatcttgta tgcccgtcaa cacagagcga atcaggaagt caatgaaagc ttgcatcgac
     5641 tggttgactt tttaagcacg caagacatag aaatctttca ggactcagat actgcggcca
     5701 gttttgaatt aaaagcccct gttttaccta gagaaaaaat gggggcaaaa cacgacttga
     5761 ttattgtcgt gggtggtgat ggcagtttac tttctgcctc acgtatggct ataaaagtta
     5821 atacaccggt catagggatt aacaggggga ggctaggttt tttaacggat attttacccc
     5881 aagacattga gtcccattta ggccctgtgc ttaatggaca atataatgaa gaagaacgct
     5941 tcttattaca tactaaaata tatgataagg aaaacagtta ttttgaaggc gatgcactca
     6001 atgatgtcgt attaggcaga ggaagtgaaa cgcatttgat agaatttgat gtttacatca
     6061 atcagcagct ggttagccat taccgttcag atggaatgat tttatccact ccaactggct
     6121 caacagccta tgccttgtct gcaggaggac cgataatgca tcctcaactc aatgccatcg
     6181 tcctggtacc catgttttca cacagcctaa gttcccgacc actcgttatc gatggggaag
     6241 cagaaattga attgtatatc agcaaatcaa atgaaaccga tttgcgtatc agttgtgatg
     6301 gtcacgaatc ccgtgtcgtt aaaccgggac agaaagtagc agtcaaaaaa aacggtaatc
     6361 gattgcgttt gttacaccct cttgattatc attattatga tacattacga agcaaacttg
     6421 gttgggaatc aaagcatcag ggataaaaca tgctcacgac attgcgcatt gaacattttg
     6481 ctattgttaa acaactggaa ctcgatttta cacaaggcat gaccaccttt acgggcgaaa
     6541 caggcgctgg caaatccatc atgattgacg ccttgctgtt agcattaggt gaaaaagcag
     6601 atccttctgt agtccgcccc ggcgaagaaa aatgtgatat cacagccgga ttttgtttcg
     6661 atgaatcatc agaactcgct caatggttgg ctgagcacga tatttcttgc catgatggtg
     6721 aaatctatct aaggcgcatt atttacgcag aaggccgctc caaatcttat attaatggcc
     6781 aacctttccc tttacaaaaa ataaaggagt taagtgaaaa actggtgcac atccatgggc
     6841 aacaccagca tcaaacactc atgcaacatg caacacatag acaacaactt gatcgctatg
     6901 ccaataacca cctcttgtta aatgatgtca atacactcta taaacaacat caacaaatag
     6961 tacaagaaat aaatgcatta caaagtcagg agcaacaagc agaccgcatt aaattattac
     7021 aatttcaaat tgatgaatta caagcgctgg atatccagga tggtgaactg gaagccttac
     7081 atcaagagca tcaaattttg catcatgcca gagagtatct gcagttaagt caacagatca
     7141 atgagctatt gaatagtgat gatcaaccga atatttgctc tggtttaaac cagatttttc
     7201 aactcatcca gcaactccct caagaacaag cgcaaataaa aaacacagct gagttaatta
     7261 attcagcctt gattcaatgt gaagaagcaa tcactgaaat gcagcaattc gcagataaaa
     7321 ttcagctgga tccggaaaga ttgcaagaag tggaagcccg aatcagctct attcatcaac
     7381 tagcccggaa gtaccatctt gatagcaaat cgcttcagga gcatgaaaaa acattacaaa
     7441 atgaattgga aagtttgcaa aatgtagaaa gcaaattggc acatctgcat ttgcaattgg
     7501 ctcaaatcaa taaggtatat gaaagcgccg cattaaaatt aagtgaatca cgcaaaattc
     7561 acgcaaaaaa actggctcaa gaaataacgg ccagcattca gcagttaggg atgccaaaag
     7621 gatggattga aattgaaatc actcctttag aaaaaatgca agctcacgga ttggataaag
     7681 tggaatacaa ggtatgtact aaccctggga tggaacctga ttcattgaac aaaattgcct
     7741 ctggcggaga attatcccga atcagtcttg caatacagat gattacagca caaaaaggtt
     7801 caactcctac cttattattt gatgaggtcg atgtgggtat tggtggctcg actgctgcac
     7861 tagttggaaa aatgctgaga aaacttggag aaagattgca ggttttttgt gttacccacc
     7921 agccgcaagt tgctgcctcg gcacaccatc actttttagt caccaaacat agtaataaca
     7981 atcaaacttt tactcatatt tcattgctta aagcatccga taaaattgcc gagatcgctc
     8041 gcatgatggg tgggctcact ataacagaac aaacactttc tcatgcgaaa gagctgatat
     8101 cgctgagcaa tgagtgaaca cgtcgatcat tttcgggtga aattttacca atctattgat
     8161 ttaatttcct attttttata caaaatctaa attaattcaa aaaattaaaa aaaaattaat
     8221 aaaaaaacag cacaattaat aaaataatgc tacaatggat gcgtaacaat ttaggaaatt
     8281 aacatgtcag aaaaaattcg tggtacagtc aagtggttta atgagtccaa aggtttcggt
     8341 tttttagaaa gtggcggcaa agattatttt gtgcatttta gtgcgatcca aggcagtggt
     8401 tttaaaaccc tggctgaagg cgctactgta atgtttaaag cttgcaatgg tcaaaaagga
     8461 cctcaagctg aggaagttga agtagtctaa tttttttata ttagccctgt ttttttaagt
     8521 ataatttaat ccttagaaaa gcagggcttt ttttatctac aacattctta aaatttctgt
     8581 tctgcactta aaaaaactct gctcaagcaa ttgctttggc aaatattggt taaagccagc
     8641 tcaattatat gataaagttt gtgccatcat tcaattcaat agcgctcaaa ttattattgg
     8701 caaatacctg tgctgataat agctgttact agcattctgt ttttcaatgg aggcagcata
     8761 gtagataaat atcaatcccc catcgtttct ggtagactgc ataaagacgt tcggtcatat
     8821 cttgtctatt ataaattgtt tctgtacaaa ctagtatggt tctcatcaaa ctcttgtcca
     8881 acttcttctt gaaccaattc tgttgttatt cttaccttat tagagttatc catgtcagtt
     8941 tgattggtga tggttttctc atgactatca tctttttctg cccacaactt tgctggattt
     9001 tctcgtaata tttcagaagg tgacttaact ttcatatcat gaaacacagt agaatatgag
     9061 ttacttttgc tatcaatgag atctattttc gaatgctcag attttgattg tttttgttcc
     9121 ttttttttca gtcttgaaac ggaatcatat gtaaacatac ttgataggat gtctcttaaa
     9181 aatttaccaa ttccttctct tatgaattct cctatggcac ttaaatatga accaattttt
     9241 gagagtatac tctcaaaaaa gtaagattct gtttgtcttt ttgatgaaga cttgttccct
     9301 tgtttttccc tctcttgctg acgttctttg cttgtttttt taatttgaga gggttgaagc
     9361 aatacatctt gatttttcgg attgatttgt tttagctcaa ccattaggtt tttcttttta
     9421 ttgtaagcat cacctgcttt gtcaaggtat ctaaatatat taagattcat ctcttgttca
     9481 gcttctgttc ttagataaaa gaaatgagcc tcgggcagtc ctttagcacc aacttcggga
     9541 tggaattgcg tggttataat aggaacacca aactctgatt ctgctgcttc attggactca
     9601 ttatcacttg cagtacctgt atgttgaaag cctgctgcta atttggaaac aacttgctca
     9661 tgcgcgccaa aaaactccgt ttcataatgc ggggaagtaa gaggatgaga atcttctaat
     9721 ccatttaaac tcgtttttat attttgtagt tctgttttta attgattagt ctcgcctatt
     9781 tctcctacta attctcttaa atcaaacaac tcctcttcta tatttaatcc agtcaacttt
     9841 aataaaagtt gcacttcaga tttatctaac tcttctaatt ttcctaatgc atctaatttc
     9901 cctgcttcct gtactaggtt taatttttcc aatgcattta gtttttctga caatctatta
     9961 tattggttca ttctttcatt aaacgctttt ctggctgtat ccatttcttt ctggacttgt
    10021 tttccaccaa taatttgagc tattaatgaa cccttatgca ctttgattgc atcataattc
    10081 atgacttttt gtgtatttaa tttgtcagaa tttaaatcgc atatttctcc accgccatat
    10141 actgcaatga cttgatgccc accacaaaca cctagaatag gcataccttt ttctgtcgct
    10201 acatgcacta atgccaattc agcaattgtt ctggaaaaat cataattttg atcggggtct
    10261 cttgtttgat taaaaagctc aggatcaatc attgaattat taccagataa tgctaacaca
    10321 tcaacatttt taagtaattc cattgctttt gctttggcgt gtgcaaataa tctttgtcgg
    10381 ccttctttag tggaatacaa cttatcaaat tcttctttgg gtatgtcttc catcattttc
    10441 ctgtagtcgc cgtcaataac ttcggaaccg acatgtgtaa atgattcttt caacgaaaga
    10501 acactgcttc caccaacttc aggactatag gtaacaatta ctcttgccat gatcataagc
    10561 atatttgaat gagcaaatga tattataaat gatcattatt aagggatcct tatttattgg
    10621 tttggttttg agcaattcgc aatttcaggt aattatttga tgaaactgaa aattaaaagg
    10681 agttctggac aagagcaaat tatttgcagc cgtgaactat gaccttcgct ttgataccat
    10741 gggatacaat atagagcttg attagcggcc ttattcctgg aacactattt accctatttg
    10801 ttttgcctgc gatttacaac atcatcaaat ccaaactaat tgatttacaa aaacaagcgg
    10861 caaaaatcgg tgcagcaaag aataaagatt tatttaaaat gcaataagtg gatatggatt
    10921 tttgcaaata ctatcagtat tctaggcaac atgatttccc cgctgactta tttatgactt
    10981 gtaaaggatc tacggtatta atggttccac cggaaggatt ttgctttaat tatgaaacat
    11041 caaccagcaa ctctttccaa tcattcatgt ctatcaaaga cataagcact aaggcgctag
    11101 cagaatttac tgctatggtg ttacaactgg aaaaagaaga gataaaagta ttaaccctat
    11161 cgcaaagaca ggatttaccg gatgccgtct ttcccaataa ctggttttca acacatattg
    11221 atgcaagagg gaataatatt cttattattt atccattact agctaaaaac agacaagcag
    11281 aagtcaatat caaaggatta atggaagtac tgaaccaagc tcaatttgaa gttcatgaaa
    11341 taattgactt aagaaacgat aaggatgaaa tccttgaagg aactggcagc ctggttttag
    11401 atagagaaaa ccaccttctt tatgcgtctt tatccccgag aacttccctg aatctggtaa
    11461 acaaagtcgc tgatatcctg ggatacactc ctattgtatt taatagtgta gatagacaga
    11521 acaagcccgt ctatcatacc aacgtcataa tgagtctaag ccaatgctac gcgattattt
    11581 gcctggattc tattcatgat actcaacaaa aagccatgct ttcagaaagt ttcaaagaaa
    11641 caggcaaaac aatgattgac attagttgga atcaagcaca gcacatgtgt ggtaatgttt
    11701 tggaacttta taacaaaaaa ggtgagagcc ttttaatttt atccgagcaa gctaaaagac
    11761 actttactca tgagcagcta agaatcatcc aacaacactc cagattaata tcaacagata
    11821 tccctacaat agaagccatt ggaggtggaa gcgcacgatg catgatggct gagatttttt
    11881 attaaaagtt caaaaaattt tgctaccagt ttaatttcct ttcagatatt ctctaaaacg
    11941 agacttaaga aaaaccccaa ggtcaaggta aaaaaagatt ggaggtttac ttaaggcctg
    12001 taaaaaacta atcattcagg cagggaatca tcatgaaaat tagtgaatta aaaaaactgg
    12061 ttgctgaatt aaataaagag gtttcaccaa aggttacatg cacaaacata attaacctgg
    12121 ctggcaattt atcagaaatc attgagtatt ttgagcagga tgaacatgtt ggtccagagc
    12181 ttatataccg aatagaagcg gtgatttgtg aattctggaa gctagttagt ctcacattac
    12241 cttatgaaga atggcaaagc agtattcaag ttgctccctg gcttatatta cagcaatcat
    12301 tatccaaagc cgggttactg cctacggatt tccaccatcc cattttgtac cagcgcttaa
    12361 aagaacgcta tgaaagcttt ggtcacagcg agttaggagt tgaccagctc ttgcccttgc
    12421 ttatccgttg cagccgcatg actgggtatg ccaataaaga tccacaatct cttgatactt
    12481 atcctcactc accattaaac aaacaaatcg aagcgagaag accacaggag cttgccaagc
    12541 taaaagacat cctctgcctt ttaagagcca tcttctattt aattcatcat tgttgcacca
    12601 ttgagcaatt gacgttaatt ccttacctga tttattttcg taatcccact acagatgaag
    12661 aaagacgatc tgaattggcc atttttaact ggctaaccca aaaaccagct gattgccttg
    12721 aattctttaa aaccaatgaa gattacattg acacaagatc atttaggcaa attagtgagt
    12781 tggcaccatt acgccctttt atcccaactg cgcgtagtga ttttattaaa ataactaata
    12841 gagaacactg gatataccct tttattcaaa gcaggacaaa tacatcaaga tcagagtatg
    12901 atttgctgaa tgatgcagtc aattggttag atactgattt tgcaactgag aaagataaat
    12961 cctatcatgc tgctttggag tttgcccata ccgtcaaaaa gcaagcaaac attttaaccc
    13021 aaagggaaat gaaaatagtg cattccgcgc tctatgtctt ttgtctggac aaatacataa
    13081 aacatcgtaa agcagatcct cgtccaaggt gcactccgtt ttcactttcg ggagaaacaa
    13141 aatgccaagc agccgaaaaa aaacagcagg agatactggg aaaaccaacg aagtttggat
    13201 ttttcgaaaa tctggccttg aatgaaggaa gattaaaaac actgaccaag acttttgaaa
    13261 tgccacccta tcctttacta agaaattagt taaatttaat tgttgttttg actgaaatga
    13321 aagattatgt ctgattaaat caaaactaat tcgaattaga atctggtaat attggcattc
    13381 actaaaggtg cttcattttc tggagtctcc ataggcgtag cgacgggctc tggacccttg
    13441 atattttgca gcctttgttt gaatgatgaa accaaagttt cattttcgtt tttaatatta
    13501 aataatttgg aaaatagcgt tttaaagaaa ctcaccagaa aattgtcaga atttttaagt
    13561 aatacatttt tacattgatc acttaggccg tgagccttga cctctgccaa acgaatagaa
    13621 ggctctttgc tggttgtttt tagcatgtct tccatctttg atatttcctg aattttttca
    13681 tctttgattt cttttgacaa actcttatga ttactaacat gctctcttat ttctgtcagt
    13741 gagtcataca cactgatcat cgtgtcatat ttttctaatt tacccataaa acttttcgca
    13801 cttttattct gctcctgcaa aaacctgagt gtctcatgat ccggaatacg ttgggtattc
    13861 attaactgag actgatggga tcttaatgcg gctatttttt cttcacggca gggattacct
    13921 ttaggtttct tttcttctgc ctcaatcagg ctctgtactt cttttagatg gacgtttaat
    13981 tgctcgtaag ctgcttttaa ctccttattc ttttcaaaaa tagcaggagc atgaatttta
    14041 aactgggttt caagccctgt ttccatatta tcaatggcta tttctttgat tatttcagat
    14101 tttttggcaa taaaatcagg catgatttgc ttcaagaata cctttccata aggaccgagt
    14161 tcgttgttca attgctcgct gattttagta ttaatataat tagtaaaaac ttgtccttta
    14221 aatgcttctc caataagaaa gtttccttta gtttttgaca agacttcggg gttagagtca
    14281 tttaatattt gcactgccaa atttaaacgc tcggctgggt ttttatcaaa attcgagagg
    14341 atattctgaa catcggaaat catttgtaac ttatcttttt tgatttcggg taagtcactc
    14401 ccgttctcta tggtttcttt taaagaatta agtacatcat aaatattgat taaggcgtca
    14461 ttgctttcca gagatttcag cgaagacctt gtttcttctt gcatcttctt aaggaattcc
    14521 actgtatttt ctttaggaat actgtccaaa tcactcaagc gatcctttag agaagtcaat
    14581 ttggctattt tttcattgcg acaaggatta tccctggtct ttttattttc ctcatcaagt
    14641 aatgtattaa tttccttcaa tgtattattt aaatcaacgt acgcctcttt caatggattg
    14701 ttattcagca aaattccggg agtaatcgca ttaatttttg agccaatttc agcccccatt
    14761 ttgctgtcaa atgcaacatt tcccaatatt tcagattgat gcaaatgaat ttccgggctt
    14821 attttatcga taaataaact ggtataaatt cccaattcat tgtgcagaga gtttttaata
    14881 tcgctctgaa tataactgtc aaatagtaat tttttaaaag actctatttt attattgata
    14941 atgtcagggg ctattctgtc tattgctttt gcaatttcct cttccatatc ttggtcaata
    15001 gtaattttct ctaaaatctc atttttttta tcgagaaaat ccttttcaat gaattgtaga
    15061 aactgttcgg catgagaacc aattttagca tcaagggcac tattgacatt agcatggatg
    15121 taattaaaaa atattttttc tttaaaggct tgtatttttt ctggcccaac ttccagctcc
    15181 tgtttcttta gcagatcaat aaaataacct atacgctctt cacataattt tacctttaat
    15241 cgttttgtat catccaaacc ggtaatcaat tcttgaagtt tactttcatc attaataatt
    15301 ctttctgtcg cggctttaaa atcttccggt gtttgcaact ccctaagaaa ataagcgaga
    15361 tcatattggt agtttatttt gataagatgg ggttgaagct ccgcataaag ctgcaaaaat
    15421 tgttctttat tctcttcttt ttcgaaatcg ccatccaaat ataaatccct taatttatca
    15481 aaagggctgg atggtttatt aattttatga cgctcttcga ataccctgtc aatggcatgg
    15541 agtaatgagt actcaccatc tgttttggaa atgtaatcct cagtaaattc aggattaatt
    15601 ttagcaagat aaggttgaat ttttttatat tcattcgaag cttcttcttt taattcctct
    15661 aatgtcctct cgtacagatt catgtgcacc ccatcttcta tatccttcgc aatatgcctt
    15721 aagctggcat atggttgata taaagtactg aattgctcat gctctgtacc aaatatactt
    15781 tttttcgtat cgattgcact ggtatcttcc ttgacacttt caaggcgttc catttctctt
    15841 gccaaacgtt tttgtgtgct tgttgtatca attaataact ccaaacccgt ttgatctttt
    15901 tccaaaggta aattgacaat cagagaacta tagaactttt caaatcgctc actcacagta
    15961 cgagaatacg taccgggctt taggcccaaa tgaaattctg cattatcagc agccgccatt
    16021 aaaatggtgc cgaactctgc ttgaatttct tttaaacgcc ccattgttaa ttctcgtcct
    16081 tctttaccaa tatcagaaag ctgcatctgc agtttagcgg cagtactcag aatcttttta
    16141 actgaacctg gaccaaagga taatccattt aatccttcaa caaatgcttt cacattcgca
    16201 tctatttcct ctttggaagg ttctggagtg tcaggttccg cttttaactt attgagcttg
    16261 actggaattt gatagaggct ttctgcaatt aattgtaaat aagaagtatc tttcagtgac
    16321 gtactttgtt gaagagcatt ttcctcattc ataattcttt gcaaagcacc actgaaatcc
    16381 tttgctgtct ttaaaccttc taaaaattgc gtttgatcat aagtactatc gattttatgg
    16441 aggtacggct gaatttcctt gtatagctgt cgaaattttt ccttgttttc ctctttttca
    16501 aagtctcctt ccaaatgcaa atccttaagt ttagcaaaac tactttcagg attattaatg
    16561 cgttggcgca ttgacatcac ttcatcaata gctttcagta gtgttgatga ctctttatgt
    16621 tttgctatga aatttttatc aaattttgga tcaacagact cgagaagagg ttggattttg
    16681 tcataataac taaccgcttc ctcttttaat tcgtcaaaag aaggtttata tggattattt
    16741 tgatctcgaa actcatcagt aatacgcttt aacaagagat agggctgata aacgctatta
    16801 aattcttcat gttttgtgcc aaaaactacc tcatgagggt tagttgtacc agtaataaca
    16861 ggagtaacag gtttttctgg tgccttaacc tttggtgccg gttcttgttt taaaggcaga
    16921 ttactaacaa gaccattaca aaaattactg acaatagtgc ttatgttatt ggagtaggag
    16981 ccaaactttt gacctgaatg atactcggta tattctgcag ccgccctgaa ttgcgcattc
    17041 aattcggaat gaatgtcctt aagtttacca agtgttactt caggactttc cttatccaga
    17101 ttagaaagct gggtttcgag tttggtcaag gcagccagta tttttttcac agaaccaggg
    17161 ccaaaagaca agccactgat ccccttggca aattcgccaa tttgagtttt tattttttcc
    17221 ctggctgaat ctgaaacttc caggtcacca tcaaaattat cgatggcttc ctgaatttta
    17281 cccaattgct cactaattaa ttgctgatct gttttaggct taggtccttt tgacaaacca
    17341 gattctacaa ccgcttgttg tttcttccat gcagtaataa tattttgctt ggggggctct
    17401 tgtttttctg tggtttttaa ataatcacct atgagtggca taccctctaa tggttttagc
    17461 aaatccaaac cctcttggac aattgccttt aatcctggat tatttcctgc ctccataaca
    17521 tagtacttac tgaaacagat attcataacc agagcattaa atgtggtcat gacaaatcgc
    17581 gtacggtgat aaatgttatt ggggtcacca tagtcattta acgactccag cttttccaag
    17641 ccattcttca tataatgcat ggagtttatt aattgcttgt acatggcaac ttcagggctg
    17701 tctttatgaa actcgagata cggtattttg tcaaaatcaa gcgtttctcc attcgatgag
    17761 agctgtttcc atacctcagg agaaagattg tccttaagat aagtctgaaa ttttttattg
    17821 aaaaaatcat caacagactt ggataacttc aatttactca tctgcccaaa gagcgtttgt
    17881 ttctcaagtt ctgccccctt cgctaccaag ggattttttg catattgttc ttcgcgagct
    17941 tcttcctcct tatccagcaa agtcgtttta tcctgattta aatcactaat tttctcattt
    18001 aaatagtcat taatacctga tttaagagat accagatcgg tgaggcgaag aggagggtcc
    18061 gttggttttg aactggttag gctgcttaca aggcgagtgt taatatcatc atgcttcaac
    18121 gccaataatt gtggttgaaa tgttttataa gccttgcgaa gaaattcttt atccgattca
    18181 tccagagaat caaacgatgg gttttcatgg ggatactttt tctgaatata attaaaaaac
    18241 tccgctactc ctttctgtgc tctttcaagt tctaaaattt ggttagatgc tgcaataacc
    18301 ctcttatggt aatactcaac cggtttatcg tcctccaaag taaattttag tggttcaaac
    18361 ggttgaaccc gagtagttag attagttgtt ttattattca ttgaataagc tgtctcttct
    18421 gagtgactca tggttttttc aatgagcttt gctttaaact cagattgagc aaggctttgc
    18481 tgaatactgg atagcacaga gtccttacag gataatacct gcttaaaatg atcagaactc
    18541 cataattgct tataatcgga actgtatagt ctggaaacga tattgcttcc ggtaggctga
    18601 gtaagagcat ctacaaccag tttgtcaata tctgggtata atgccgcaaa atgtggctga
    18661 aactgtttgt actccttgat taaagcgtcc ttttcactct gactaatgtt tgccaaactc
    18721 cacttggacc atgctttgtg aatgctattg taggagccga ttttatcaaa gaaacgattg
    18781 gccgcggcaa gtacactttt gtcttcagag ctaagtctgg cttcattaag tcggctttcc
    18841 tgattagatc ttctcttctg aataaataca tcgtccatca aaacacctaa atcaggtgca
    18901 ggcgttaatt ttggccccac atccagtttt ttgctatctc cagcaagaaa acgaacgatt
    18961 tttcccccta ttgaatcaga ataatctgaa acagtatcaa gaactccagc tgccttggca
    19021 atattatcaa cttgctctgc cagctgcgta tagtatttat tcatttgttc aagagcggga
    19081 tcggtcagta aacctggttt taaacccatg ctttcttcaa ccatttccag ctctgaaatc
    19141 aattggggta aaatattatg tttaatgtct tcaagcttag cgacagcatc caaataagct
    19201 tgttttgtca gaggagcacc ggcattaacc aaatcagtac tatgggcaat taatcttttg
    19261 actattgaaa gatagcttgg gattgctaat agcccactct tggaactgag ttgttcaaaa
    19321 taatattttg tttcatttgc cttttttatc attgcggatt gatagtcttc agcactggta
    19381 attgatttgg tggcaatccc tgatgccccg gtagcgatta atttttgtaa ctcctcgaag
    19441 taatgaggca attcaaaaat aaggttactg agtttaccca aactttctga ctcggttggc
    19501 ttttcagttg gaagcatatt gactacacca gcaagcactg ccccggcact ctcctcagga
    19561 tgctccggag caaaatttcc taatgcctta ctggctaaag ccatttgtgg taataatttt
    19621 tggatatgtg ggcctactat atcttgtatg tccgaattac tgtgattgat tagttgaaga
    19681 gccgcataaa tttcatgcac cgctttataa ctgaccatga ctgcatcttt agcgatcatg
    19741 gcagtgtacc tatcacgact gatatcaata ttttcaatag ctcgaaacga cttttcagca
    19801 ttttccagag cattaagcaa ttttttcaga acagcgactt gggtaggatc agtcgtgtta
    19861 actttaaaag gggcattggg gttatctcgt tcaaaataag tagccaattc ctcagaaaaa
    19921 tgggttttat cggtaatcga ttttaacaaa ccatctttta ttgttttaca agtatcagct
    19981 atttttaaat cttcttttaa atagatgatg taggtttcaa tggttctttt catgccacat
    20041 caaccag
//
LOCUS       DACTJE010000046        19542 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.046, whole genome shotgun sequence.
ACCESSION   DACTJE010000046 DACTJE010000000
VERSION     DACTJE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19542
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.046"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..480)
                     /locus_tag="I9760_001273"
     CDS             complement(<1..480)
                     /locus_tag="I9760_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HAT5043049.1"
                     /translation="MPNSSNVLRHMENHFALTTCEDIKNIMQPLLIKHGMTVFNYYRI
                     YFDSTVIRLSTDQKWTEHYFKMNYLETLTIPESYLKKPLNYYLWLTDDCPLMLQDAAI
                     NFNMANGISIAKINHDSIEYFCFASTRDNTSIVNNFYLNNLDVLEQYSLYFKDQFNSI
                     "
     gene            complement(743..1489)
                     /locus_tag="I9760_001274"
     CDS             complement(743..1489)
                     /locus_tag="I9760_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal large subunit pseudouridine synthase
                     B"
                     /protein_id="HAT5043050.1"
                     /translation="MSSERLQKILSQAGLGSRREMERWIDNGWVQVNGKPAKLGDSAG
                     PSDKISVKGKLIPNPLKSKQNIRVLLYHKPVGEVSSRHDPKFEKTVFDNLPHLRQGRW
                     VQVGRLDLNTSGLLIFTNNGELANQLMHPKYGLEREYAVRVHGQVSQESLNNLLKGVM
                     LDDGLAKFTKIQFRGGEGTNSWYHVTLNEGRNREVRRLWESQGVEVSRLIRIRYGMLT
                     MPRFLSRGQFYELTPKEVTEFLDSLPNELS"
     gene            complement(1479..2060)
                     /gene="scpB"
                     /locus_tag="I9760_001275"
     CDS             complement(1479..2060)
                     /gene="scpB"
                     /locus_tag="I9760_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAT5043051.1"
                     /translation="MIDNELKNIVEALVLSSNEPVTLEKIQETFDEWQRPTLEDLQAI
                     IESLKVDYSYRAFELIQVAGGFKVQTKSKYATWVARLQIEKPAKYSRALLETLAIIAY
                     KQPVTRADIEDIRGVAVNSQIIKTLMEREWIRIAGYKDVVGKPAVYTTTKEFLNYFNL
                     NYLSELPPLPEIMDTLTLHEATQPIKECTQNEQ"
     gene            complement(2062..2853)
                     /locus_tag="I9760_001276"
     CDS             complement(2062..2853)
                     /locus_tag="I9760_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAT5043052.1"
                     /translation="MNIDLQPAPVVKAVVDGKEITQFPEDLYIPPDALEVLLDSFTGP
                     LDLLLYLIRKQNIDILDIPMTSITNQYLQYIQLMENRRMELAAEYLLMAAMLAEIKSR
                     LLLPVTPDSEEEEEDPRMTLVRQLQAYEQIKQAAELLDGLPRQDRDNFTIQVNPDEVE
                     AIKVYPEVELSELVEAMKILLKREEHYSKHQVSRELMSVRERMSRILFQLQHQKILEF
                     GQLYTLEEGRMGLVVSFLAILELAKQSLLIITQTQPFSPIHLQAA"
     gene            complement(2853..4067)
                     /locus_tag="I9760_001277"
     CDS             complement(2853..4067)
                     /locus_tag="I9760_001277"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAT5043053.1"
                     /translation="MSALFTSNKRVVSGMRVSGRLHLGHYHGVLKNWIKLQHQYDCFF
                     FAADWHGLTTQYEDPNFIEKHLWDMIIDWLACGVNPGLCKIFIQSWVPEHAELHLLLS
                     MITPLGWLERVPTYKDQQEKLKEKDLSTYGFLGYPLLQSADVLLYHADYVPVGEDQVA
                     HIELTREIARRFNYLYGREANFEELAAAAIKKMGKKNGKIYTELRRQFQEHGIHESIK
                     TAQALLEDQPNLSIGDKERLLGYLEGSGKIILNEPQPLLTETAKMPGLDGQKMSKSYH
                     NTIMLRDEPALVEKKILTMPTDPARVKRTDPGEPEKCPVWQFHKVYSNEEVKDWVQKG
                     CRTAGIGCVDCKRPVIGAIHQELKPIQEAISDYEADLGSVKRIVAEGSEAAREEGNKT
                     LNTVREVMGLDY"
     gene            complement(4313..4933)
                     /locus_tag="I9760_001278"
     CDS             complement(4313..4933)
                     /locus_tag="I9760_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAT5043054.1"
                     /translation="MSQFFALHPDNPQARLLRKAVSIIEEGGLIVYPTDSGYALGCSL
                     GNKSALERIRRLRQLDKHHNMTLVCRDLSQLGTYAKVSNPIFRLLKAFTPGSYTFILN
                     ATHEVPRLMLHPKRRTLGLRVPDNTITLSLLECLEAPLMSTTLILPGAEAPLSEPEAI
                     KDLLGNQIDLIIDGGNCGHEPTTVVDLTGDYPIIIREGKGDPEPFK"
     gene            complement(4930..5769)
                     /locus_tag="I9760_001279"
     CDS             complement(4930..5769)
                     /locus_tag="I9760_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAT5043055.1"
                     /translation="MIDLHCHSSFSDGALTPEELIKRASTLQVECLSLTDHDTVAGYD
                     ALYDAAVHTNIKIVKGIELSTRWKKYDIHILGYQINHTAELKELIAHQNKSRIERAEK
                     IGESLHSVGIDNAYEKASLLAGHKRVGRPHFAQVLINEGKAKDMKSAFKNYLGRGKCA
                     YIPTLWISMHDAVKGISHSGGQAVIAHPLKYGLTRSKLRDLITEFKEAGGVGMEVVSG
                     EMTTVQIQEMAGMCERFHLLASSGSDYHSDAQSCINLGRQRQLPIICTPIWQEWNMKQ
                     GTL"
     gene            6048..6998
                     /locus_tag="I9760_001280"
     CDS             6048..6998
                     /locus_tag="I9760_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAT5043056.1"
                     /translation="MSSTLNSEIIKLPPLNTRELANLCGVLHSNLKILEQHFKVSIKI
                     KNHSMIICGQSGEHFEEIKKVLKLLYPLTSEPIDTKTLRVLINEDYQTAMTHHIRLSR
                     KSISARNSKQAAYLDSINTHDITFAVGPAGTGKTYLAVSKAIECFEKGEVQRLIFVRP
                     AVEAGEKLGFLPGDLVEKVLPYLRPIYDALYEMIGFKETQKLIQTDIIEILPLAFMRG
                     RTLNESFIILDEAQNTTIMQMKMFLTRMGFGSKTVITGDITQVDLPKGIDSGLAHAIQ
                     LFRHFPDISIHTFTSREVVRHPLVSKIVDCYDNEMERIKK"
     gene            6995..7471
                     /gene="ybeY"
                     /locus_tag="I9760_001281"
     CDS             6995..7471
                     /gene="ybeY"
                     /locus_tag="I9760_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAT5043057.1"
                     /translation="MTYHIDIQNATGKLLPLSEDEITKLASLALRDHKQDAELTVRLV
                     DVEEMTYLNHTYRKKNKPTNVLAFPCSLPANIELECPLLGDVVICPEVLLAESAQFNK
                     SLHAHWSLILIHGVLHLLGYDHIKDEEASIMQMLEAKLLAELGYANPYEVEENELE"
     gene            7431..8321
                     /locus_tag="I9760_001282"
     CDS             7431..8321
                     /locus_tag="I9760_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAT5043058.1"
                     /translation="MLIHMKSRKMNLNKLEDGGSWFSRLKQFLQGEPQNQEELVSLLR
                     DAQIRSLINSETLAMIEGAILFSKMRVRDIMLPKNQMVCINKDDDFKHIISIVTQTGH
                     SRFPVTGENSDEVVGILHAKDLLKYQPENMESFDLLDICRQVTFVPESRRLDSLLSEF
                     RSNRNHMAIVVDEYGEVSGFVTIEDIIEQIIGDIEDEFDIDEDAYIKTHEGHCYIIKA
                     HTPIEEFNEQLNADFSDETYDTIGGIVLNKFGYLPQRGEVIIIDDFEFKVLNADSRRI
                     KLLECLDKRSPEKLNSTVEG"
     gene            8329..9009
                     /locus_tag="I9760_001283"
     CDS             8329..9009
                     /locus_tag="I9760_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT5043059.1"
                     /translation="MSSSILIIDDDTELTDLLTQYLEPEGFNVVCVHDGENGVKRALN
                     QVFDAIILDVMLPKLNGFEVLKAIREHLETPVLMLTARGDDIDRIVGLEIGADDYLPK
                     PCNPRELVARLRAILRRTQKIPTPKPIIEQHNIIVDCSKRHVTMGGKFLELTNAEFNI
                     LEMLIKSPGQAFSKEELTEYALGRKYTAYDRSIDVHISNLRNKLGDNPQGEPLVKTVR
                     GFGYMFNA"
     gene            9002..10369
                     /locus_tag="I9760_001284"
     CDS             9002..10369
                     /locus_tag="I9760_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HAT5043060.1"
                     /translation="MRSLYWKIFVSFWLATILIIFTTAWVTSQIAQKSSQPAREEMFM
                     DSYANAAVATYESGQQSALLKWLDKIGLSRHMTLYLLTSTGEIIGAQKPPDAVKKISE
                     NLINDELPSDGIFKTGKLVVSHEILSTSGKYYRLAAVSEKPISYFVQIPWAGLTIRLT
                     LAIFISGLICYLLSRYLTQPLRSLGMAAKSIAKGNLNTRVGRFRGHSKDEIAELSNEF
                     DRMAEQLEALVHSKERLIQDISHELRSPLARLKIAIELGKKKTKHLADNEFARMEIET
                     SRLNDLIGEILDFARLEKSTTELHLSQTNIADLLAQVISDANYEFSHNSIRIQAGIIT
                     PCELNIDQRLIHRAIENIVRNASHYSPPDEKVLISSSYDKNKDQVYIDINDKGPGVPE
                     DQLEKIFNPFYRVDTSRTKKTGGYGLGLAIAARAVALHQGEIIAKNREQGGLLVRIIL
                     PADVK"
     gene            10547..11350
                     /locus_tag="I9760_001285"
     CDS             10547..11350
                     /locus_tag="I9760_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043061.1"
                     /translation="MSNDTKLNNASNYFFISGFVISKIKYLPIAVVSSALNLISLLFY
                     LVGYSLWFIASHFYPGQDKKMQEWYEFAQFKEQYLYAAALGLVATSISMMAIFSPIML
                     VLSGWLFFGSNIVWTIGEYNKLNNPPSHEENFSKDRQNAYVSYALSISLIGFITAVST
                     TVAFFIPVMTIPLLIVTTIVCIGVGALAFEYWIESKFGDSQPDITIDESYKQMSNDLE
                     KKITLEPNPTPEPYHGTRPLHFTPHDVKMKEPSPDLQMDLSSRTCKPQY"
     gene            11389..11784
                     /gene="cdd"
                     /locus_tag="I9760_001286"
     CDS             11389..11784
                     /gene="cdd"
                     /locus_tag="I9760_001286"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAT5043062.1"
                     /translation="MDKLTSNMINEAHKVLVNAYAPYSKFSVAACICTDKNNFYTGVN
                     VENISYGLTTCAEVSAICNMVTAGERKIKSIVVLAANNLLCSPCGACRQRIHEFSSSD
                     TMVHLCNKETIFHSITINELLPLAFDFNP"
     gene            11797..12636
                     /locus_tag="I9760_001287"
     CDS             11797..12636
                     /locus_tag="I9760_001287"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAT5043063.1"
                     /translation="MTTSISTISYPHLAAEYIQKTCPSFKPKVGVVLGSGLGQFAEEL
                     EDTVAIEYEKLPGFPRTTVQGHGGKLILGYYGSTAVICLQGRAHTYESMENHEAVKTY
                     VRTLKLLGCQYFIATNASGSLKEEVGPGELMLITDHINFQPGNPLVGPNDDEFGPRFY
                     PLDNAYDITMRNALLDIAQRHSIKLHQGVYISVLGPNYETAAEIRAFKLLGADAVGMS
                     TVPEVLVANHCGLRVAVIATITNYATGLAKTSHSHESVVKMASSAAEKLNTLIKQYIL
                     ELS"
     gene            12633..13397
                     /gene="deoC"
                     /locus_tag="I9760_001288"
     CDS             12633..13397
                     /gene="deoC"
                     /locus_tag="I9760_001288"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAT5043064.1"
                     /translation="MSLDKHFNDLLDKLLSNQDITSISNAQLMHCIDLTLLEADASLE
                     ALHQLQSQACQNNVAAICVLPKHLHVFKPNSKIKLATVINFPLGNDDLLVSLAEIDNA
                     IQLGANEIDYVLPYQYYLRGNKQKALNHCDVIIQTCKKHQLTLKIILETGLFPDMESI
                     YQLSSEIIALGGRFLKTSTGKTPQGATLAAVFAIVSAVMDSTYASSCGIKVSGGIKTP
                     LQAQQYAQLAELLMGKAIHKDWFRIGASTLLEKLLS"
     gene            13451..15223
                     /locus_tag="I9760_001289"
     CDS             13451..15223
                     /locus_tag="I9760_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAT5043065.1"
                     /translation="MKLWIMLFNCFIVTFLITFSHAKAPVNNNKSSYCLPNQPCWPTP
                     KEWEQLNKKINGHLIKARNYLSPCIEDPKSNGCKNTLEEIKNPFYLETQPTATQSTGW
                     FEAWDSEISPYIVAAKTAQEIAHAVKFARKHHIKLVVKGTGHDYLGRSNAPDSLLIWT
                     HHMREIQFQKHFVPNGCSANHKGVPAVTVEAGTRWIEAYKEVTVNQGRYVQGGGCTSV
                     GVAGGFIQGGGFGSFSKKYGTGAGGILEAEVIIADGSILIANECQNQDLYWALKGGGG
                     GTYGIVSKVTLETHELPATFGALTGSITAKTEQDFKNLIDYFIIFYRERLHNEHWGEQ
                     VILGPDNKIELALVFQGMSKTQVDRLWQPFKTWLSQKPEHYQFTINSLVLPARKFWDY
                     DYLSKNLPDHIVIDKRKNIPYPEFWWSGNQSEVSIYLTHYQSGYLPYTLFQEKFSNKL
                     SNALFEASRLTKLSLHFNKGLAGASQQAIERQRKTSMNPSVLDAAALITIIGGQQYVF
                     PKLPEHKPDLDKARFSAKQAKKAMKLISALNPDSGTYGNEADYNLKNWQRALWGSNYE
                     KLLQIKHHYDPENLFNCHHCVGSE"
     gene            15303..15701
                     /locus_tag="I9760_001290"
     CDS             15303..15701
                     /locus_tag="I9760_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043066.1"
                     /translation="MKTKIIFKSISSLLLGLLLVSCSNELPPGEYDANEIGKIKKVIP
                     GVIISKRPVNLHRQNADANLHVKGGADSNLIDGGYNRTHGVEYVIKLNSGTIISVVQA
                     EDLKLKNKQKILVIYGRHTRVVADNGSDVY"
     gene            complement(15835..16683)
                     /locus_tag="I9760_001291"
     CDS             complement(15835..16683)
                     /locus_tag="I9760_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAT5043067.1"
                     /translation="MIPWNAYVRLLRLNKPIGILLLWYPTAWALWMANQGFPSIDLLM
                     IFLLGTVFMRSAGCVINDIADRHIDRHVARTQFRPLTAGEVSLSEAFILLFILLCASL
                     LLLLKLPINCFYFAVISVLITFLYPFCKRFLNAPQLILGLAFSMGIPMAFIASGKNLN
                     SDFIVLFLINFSWIIAYDTMYAMTDKADDLKIGVKSTAIYFASYDRLIIALLLIFLHS
                     LWLVWAINKNAEWFFYLLWCTAAGILTYQLKLIYARIPKNCFKAFLVSGYYGLVMWFA
                     VGLALI"
     gene            complement(16680..17216)
                     /locus_tag="I9760_001292"
     CDS             complement(16680..17216)
                     /locus_tag="I9760_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAT5043068.1"
                     /translation="MSINYESLLIADTKGPGLLKNWLEYQDSLTDKLKAITGNAELER
                     LSQNWSIPNWWDKYVLCIPDKSILQREIVMKNQGIIYWYARSVIPQSCYALKPEFFNR
                     LENESIRNLIFDESSVRRLPILCYPVDQLNLEFHWVKKYISAEYSQMWVRFTELVFQE
                     KSSFYLVEILLPELENLL"
     gene            complement(17329..17817)
                     /locus_tag="I9760_001293"
     CDS             complement(17329..17817)
                     /locus_tag="I9760_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAT5043069.1"
                     /translation="MRLQLMKITGFTLLETMLTLALLVGLLLLGSWSFFSLIQNNERE
                     TLVNSIKTAIQYSKIQAIHLGHPIYLLPFGSNENWSRGMVLAKLNQTTNKTELIHQWQ
                     WSSNSWNINWKGVDSNHRIIISNIPNRAMSNGKFILNNKRTNEKVVVTLNRLGRVRVG
                     GN"
     gene            complement(17823..18602)
                     /locus_tag="I9760_001294"
     CDS             complement(17823..18602)
                     /locus_tag="I9760_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAT5043070.1"
                     /translation="MMTLITGATRGIGLALAYEFASHGHDLILIGRDSEQLEHISKQL
                     NTQYQVNVEYKVHDLSKPGSAFKLYTDLKEQGVEVNCLVNNAGIGYMGSFVEMGMNKL
                     DDLMNINMISLSELTLCFLHDFVARNEGSVLQVSSTAAFQPGPFMAAYYASKAYVATL
                     SHAIAYELKDTKVSMSILCPGATKTNFFHASGMESSMLERGYIGMMTPEKVAKIAYKG
                     LKKGKLYIIPGLRNKILAYAAAISPKAIALRIASYLHHKIL"
     gene            complement(18547..>19542)
                     /gene="vpdC"
                     /locus_tag="I9760_001295"
     CDS             complement(18547..>19542)
                     /gene="vpdC"
                     /locus_tag="I9760_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VpdC"
                     /protein_id="HAT5043071.1"
                     /translation="DPASGWERDRLKLRQYALQTIIPETGKVSTTGFNVERQSQEELI
                     QNGYQSTLDYLNVRYTRKNNSYVNKEIMYSTFNSLGDLLAYCCYRGDEQWFNVVNNLI
                     VQSTLPNRTALMKQSLELRKLYFNTSLPSNPEKNKTACNESNTLTFFGNAVCQHHNHS
                     QELENGNHDVLLALYPIFLKLSTDLLINKIDKNLLDRARHSLNIHSPFDCLEHFNKIS
                     GNAHVMIHIIVKLIKELKERSCQQIYDVLKEVRHLLYSDINLMKANYFGKWDLGLPQC
                     IRILNLLKQEKHDDVSRLLDCLRNQMEPVQTVKGGVHHDDLNNGSHERYRFGLGV"
BASE COUNT         6269 a         3762 c         3462 g         6049 t
ORIGIN      
        1 tattgaatta aattgatctt taaagtaaag actatattgt tctagaacat ccagattatt
       61 gagataaaag ttattaacaa tacttgtatt atcacgtgta cttgcaaaac aaaaatattc
      121 tatagaatca tggtttattt tagcaataga tataccattg gccatgttga aatttattgc
      181 tgcatcttgg agcatcaaag gacagtcatc cgttagccat aaataataat tcagcggttt
      241 ttttaaatag gattctggaa tagtcagtgt ctccagatag ttcattttaa aataatgctc
      301 agtccatttt tggtctgtgg agagtctaat gacagtacta tcaaagtaaa ttcgataata
      361 attaaagact gtcataccat gttttatgag caaaggttgc atgatatttt ttatatcttc
      421 acaggtagtc aaggcaaagt gattttccat atgccttaaa acatttgatg aattaggcat
      481 tacttctctt ttcaaactca tgggcaaata atcttgcgta gattttcaga tctggattca
      541 gcattttttg gaaagccatg taaattgctc cagtgcttct tcagatgtta aattttcgtt
      601 ccctaaaatt tcttcaactt tgaatatgtt tatacatgtg aggataggat taatcaaaaa
      661 taactgtcca gaaagtcatc attcttttca agacagttga tatagtgaat acaacgaaat
      721 ctaacatctg attttaaatc atttaagaca attcgttggg taatgaatca agaaattcag
      781 tcacttcttt tggggtaagt tcataaaact gccctctgga taaaaatctg ggcattgtaa
      841 gcatgccata acggattctt attaatcgac taacctcaac accttgagac tcccacaatc
      901 gacgtacttc tctatttctt ccttcattaa gggtcacatg gtaccaggaa tttgttcctt
      961 ctcctccacg aaattgaatt ttggtaaatt ttgccaaacc atcatccaac ataacccctt
     1021 tcaataagtt atttaaagat tcctggctga cttgaccatg aactcgtacg gcatactctc
     1081 tttccaaacc atacttggga tgcatgagtt ggttagccaa ttcgccatta ttagtaaaaa
     1141 tgagcaagcc ggaagtattt aaatccaagc gccccacttg cacccaacgc ccctgtctaa
     1201 gatgcggtaa attatcaaat actgtttttt caaattttgg atcatgtcga ctggatactt
     1261 caccgacagg tttatggtaa agaagaaccc ggatgttttg tttgcttttt aaagggttgg
     1321 gaatcaactt tcctttgacg ctaattttat cactagggcc agcggaatct cccagtttag
     1381 ctggtttgcc attaacctgc acccaaccat tatcaatcca ccgctccatt tcacgacgag
     1441 atccaagtcc ggcttgactt aatattttct gtaatctttc actgctcatt ttgcgtacac
     1501 tcttttatag gttgggttgc ctcgtgcaag gttaatgtat ccattatttc aggtaaggga
     1561 ggaagttcac ttaagtaatt taagttaaag taatttaaaa attcttttgt cgttgtataa
     1621 acagctggct ttcctactac atccttatag cctgctatac gaatccattc cctttccatt
     1681 aacgttttta taatctggct gttaacggca actcctctaa tgtcttcaat atcagcgcgc
     1741 gtaacaggtt gcttgtaagc aattattgcc aaagtttcaa gcagtgcgcg cgaatacttt
     1801 gctggcttct cgatttggag acgagcaacc caagtggcat atttggattt tgtttgtacc
     1861 ttaaaaccac ctgcaacctg gataagctca aaagcacggt aagaataatc tacctttaag
     1921 ctctctatta tagcctgcaa atcttctagt gttggtcttt gccactcatc aaaggtttct
     1981 tgtatttttt ctaaagtaac gggctcatta ctggataata ccaaagcttc aacaatattt
     2041 tttaattcat tgtcaatcat ctcatgcagc ctgcaaatgt ataggtgaaa atggctgagt
     2101 ttgcgtaatg atcaataaag actgcttagc cagttcaaga atagctaaaa aactcacaac
     2161 caatcccatc cttccctctt caagagtata taattggcca aattccaaaa ttttttgatg
     2221 ctgcaattga aagagaatac gactcattcg ttcacgaaca gacattaatt ccctggaaac
     2281 ttgatgtttg gaataatgct cttcacgttt tagcaaaatt ttcatagcct cgactaactc
     2341 agataactca acttcaggat aaactttaat tgcttcaacc tcgtctggat taacctgaat
     2401 tgtaaaatta tctctgtctt gtcttggcaa tccatccaat aattcagccg cttgttttat
     2461 ttgctcataa gcttgcaact gtcttaccag agtcatacgt ggatcttctt cctcctcctc
     2521 tgaatctggt gtaactggca ataataaacg tgacttgatt tcagctaaca tggcagccat
     2581 taataaatac tcggcagcca gctccattct tcgattttcc atgagctgga tatattgtaa
     2641 atactggttg gttatacttg tcatgggaat atcaagaata tcaatatttt gcttgcggat
     2701 taaatacagc aataaatcta atggcccggt aaaagaatcc agtaatacct ccaatgcatc
     2761 aggaggaata tataaatcct ctggaaactg agtaatttcc ttaccatcca ccaccgcttt
     2821 gactacaggc gcaggctgca aatcgatatt cattaataat caagccccat gacttctctg
     2881 accgtattta aagtcttgtt tccttcttct ctggccgctt cgcttccttc cgctacaata
     2941 cgttttaccg aacctaaatc agcttcataa tctgaaatcg cttcctgtat tggtttaagt
     3001 tcttgatgga tagcaccaat cactggtctc ttacagtcaa cgcaaccaat tcctgctgtt
     3061 cggcaacctt tttgcaccca atcttttact tcctcattag aatacacttt atggaattgc
     3121 caaacaggac atttttcagg ctctcctgga tctgtacgtt taacacgcgc cggatccgtg
     3181 ggcatagtca gaattttctt ttcaactaaa gctggctcat ctcttagcat gatcgtatta
     3241 tggtaagatt tggacatttt ctgcccatca agaccaggca tttttgcagt ttccgtaagc
     3301 aaaggctgtg gttcgtttaa tataattttg ccacttcctt ccaaatatcc caacaatcgc
     3361 tctttatctc caatcgacaa attaggctga tcttctaaaa gcgcttgtgc tgtcttaatc
     3421 gattcgtgga tgccatgctc ctgaaattgt ctgcgtaatt cggtataaat tttcccattc
     3481 tttttaccca tctttttgat tgctgcagca gccagctcct caaaattagc ctctctacca
     3541 taaagatagt taaatcttcg ggctatttct cgtgttaact caatatgagc cacttggtct
     3601 tcacccactg gaacataatc cgcgtggtat aaaagtacat cagcactttg tagtaatgga
     3661 tatcccaaaa aaccataggt tgataaatct ttttctttta atttctcctg ctgatcttta
     3721 taagtaggaa ctcgctccaa ccaacctaaa ggagtgatca tggacaaaag taaatgtaat
     3781 tcagcatgct ctggaaccca ggattgaata aatatcttac acaagcctgg attaactcca
     3841 caagctagcc aatcaataat catatcccag aggtgttttt caataaaatt ggggtcttca
     3901 tattgtgttg ttaggccatg ccaatctgct gcaaaaaaga aacaatcgta ttgatgttgt
     3961 aattttatcc aattttttaa aacaccatga taatgaccta aatgcaatct tccactgacc
     4021 cgcattccag aaacgactcg cttattggaa gtaaacagtg ctgacatcaa tttcctcttc
     4081 ttgttataaa ttaatcaatt atttcaaata caaaaatttc tttattccct gaattctaac
     4141 ctgatgcttg cttcaatcat attttatcca aattttatca agattagaga catgagatta
     4201 ataatcaaaa gctaactgga caaaataaaa ttcaatggtg tcagctctag tctggtagga
     4261 gcacataaaa ttctctttta ctaaatacta acaaaaccgc gactgtattc cattacttaa
     4321 agggttcagg atctcctttt ccctctctta taattatcgg ataatctcca gttaaatcta
     4381 ctactgtagt gggttcatga ccacaatttc caccatcaat tattaaatca atttggttgc
     4441 ctaataaatc tttaattgct tcaggctcgc ttaatggcgc ttcagctcct ggtaaaatta
     4501 aagtggtgct cattaaaggg gcttccagac actccaataa agacaaagta atcgtgttat
     4561 caggtactct taaccccaat gttcttcgtt ttgggtggag catcaaacga ggcacttcat
     4621 gtgtagcatt aagaataaag gtataagagc ctggagtaaa agccttcaat aatctaaaaa
     4681 ttggattaga aacctttgca taagtaccta actgggataa gtcacgacaa accagggtca
     4741 tattatgatg cttatccaat tggcgtaagc ggcgaatacg ctccagggct gatttattgc
     4801 ccaaactaca gcccaaagca tagccagaat ctgtcgggta aacgataagc cccccttctt
     4861 caataattga aactgctttt ctcaacaagc gtgcttgagg attatctgga tgtaaagcaa
     4921 aaaactgact cataaagtcc cctgtttcat gttccattct tgccaaatag gcgtgcatat
     4981 tattggtaat tgtctctggc gccccagatt tatacaagat tgagcgtcac tatggtaatc
     5041 tgatcctgat gaagcgagca aatgaaatcg ctcacacatc ccggccattt cttgaatttg
     5101 tacagttgtc atttctccag aaacgacttc catgccaaca cctccagcct ctttaaactc
     5161 cgtgattaag tcacgcaatt ttgaacgcgt caatccatat ttaagtggat gagcaattac
     5221 agcctgtcca ccagaatgag atataccttt gaccgcatcg tgcatgctaa tccataaggt
     5281 aggtatatag gcgcacttgc ctctcccgag gtaattttta aaagccgact tcatatcttt
     5341 tgccttccct tcattaatca gtacttgggc aaaatggggt cgacccaccc gtttgtggcc
     5401 tgccaaaaga ctggcttttt cataagcatt gtcaatacca accgaatgca aggattctcc
     5461 aattttctca gcacgctcta ttcgactttt attttgatga gcaattaact cttttaattc
     5521 tgctgtgtga ttaatttggt aaccaagtat atgaatgtca tacttcttcc aacgagtact
     5581 gagttctatc cctttaacta ttttaatgtt tgtatggact gcagcgtcat ataaagcgtc
     5641 atatcctgca acagtatcat gatcagtaag agataggcat tcaacttgta aagtactcgc
     5701 tctttttatt agttcttctg gagtcagtgc accgtctgaa aaagaactat gacaatgaag
     5761 atcgatcata aattgcagct aatttaaaaa aatcgaagta tagcataatt aacgtgagtt
     5821 cgacataaaa ggcttttatg acttttatgt cgctaaacgc acgttatccc gtaaaaaagc
     5881 aaagcttttt atacgggatc cattcaaaat acccaattct atataagaaa aaccaggtaa
     5941 aaatatgatg ttgtaaagac taaactatcc ttttctttga acaacttgca caacatagta
     6001 cttttgcact attctttaag tatggtcatt aatctttaat aaaaactttg agtagtacat
     6061 taaacagtga aataatcaaa ttaccacctc ttaataccag ggaactcgca aacctgtgtg
     6121 gggttttgca cagtaactta aaaattttag aacagcactt caaggtttca ataaagatca
     6181 aaaatcatag catgataata tgtggccaat caggtgagca ttttgaagag ataaaaaaag
     6241 tattaaaatt attgtatcct ttaactagcg aacctataga taccaaaact ctacgagtct
     6301 taatcaatga ggattatcaa acagcaatga ctcaccatat cagattatct cgaaaatcaa
     6361 tttctgcacg taacagcaaa caagccgcat atctggattc tattaatacg catgatatta
     6421 catttgccgt agggccggct gggactggta aaacctacct ggctgtttcc aaagcaatag
     6481 aatgttttga aaaaggcgaa gtacaaagac ttatttttgt aaggcctgct gtagaagcgg
     6541 gtgaaaagct cggttttctt cccggcgatc tggtagaaaa agtcttacct tacttaaggc
     6601 ctatctatga cgcactgtat gaaatgatag gctttaaaga aactcaaaaa ctgattcaaa
     6661 cagatatcat tgaaattctc ccattagctt tcatgagggg cagaacttta aatgaatctt
     6721 ttattattct ggatgaggct caaaacacca ctatcatgca aatgaaaatg tttttgacac
     6781 gcatgggctt tggctctaaa acagtcatca caggtgatat cactcaagtc gacttgccta
     6841 aaggcattga ctcgggcctt gcgcatgcca ttcaattatt cagacatttt cctgatatca
     6901 gcattcatac atttaccagt cgtgaagtcg tcagacatcc tttggtttca aaaatcgtag
     6961 attgctatga taatgagatg gaaaggatta aaaaatgacc tatcacattg acatacaaaa
     7021 tgctacaggg aaattattgc ccttgagtga agatgaaatc accaagctgg catcactagc
     7081 tctaagagat cataagcagg atgctgagct aacagttcgt ctggttgatg ttgaagaaat
     7141 gacttatctt aaccatacct atagaaagaa gaacaaacca accaatgttc ttgcatttcc
     7201 ttgctcacta ccggcgaaca ttgaactgga atgtcctctt ttgggtgacg tcgttatttg
     7261 ccctgaagtg ttgcttgcag aaagtgctca attcaataaa tcgctccatg cacattggtc
     7321 tttaatcctg attcatggag tgttgcattt attagggtat gatcatataa aagatgaaga
     7381 agcctctatt atgcaaatgc tcgaagctaa attactagct gaattaggtt atgctaatcc
     7441 atatgaagtc gaggaaaatg aacttgaata aattggaaga tggtgggtcg tggttttctc
     7501 gtttaaaaca gttcttacaa ggagagccac aaaatcagga agaattggtc agtttattaa
     7561 gagacgcgca aatacgctcc ctgattaatt cagagaccct cgcaatgatt gagggagcta
     7621 tccttttctc aaaaatgcgt gttcgagaca taatgctgcc taaaaatcaa atggtttgca
     7681 ttaataagga tgatgatttt aagcatatta tatctattgt aactcaaacg ggacattcac
     7741 gttttcctgt aacgggtgaa aattctgatg aagtggttgg cattttgcac gcaaaagatt
     7801 tgttaaaata tcaaccagaa aatatggaat catttgattt attggacatc tgccgccagg
     7861 taacttttgt acctgaaagc agacgtcttg atagtctgct tagtgaattt cgaagtaata
     7921 gaaatcatat ggctattgtt gttgatgaat atggagaggt gtcaggtttc gttaccattg
     7981 aagatatcat tgaacaaata atcggtgaca tagaagatga atttgatatc gatgaagacg
     8041 cctatattaa aacacatgaa ggacactgct atatcatcaa agcccatact ccaatagaag
     8101 aatttaacga acagctcaac gctgatttta gtgacgaaac ctatgataca attggtggca
     8161 tagtattaaa taaattcggc tatttacctc aacgaggtga agtaataatc atagatgatt
     8221 ttgaatttaa agttcttaat gccgattcga gaagaataaa gttattagaa tgtctcgata
     8281 aaagaagtcc cgagaaacta aattctactg ttgaaggata agtaattcat gagcagctct
     8341 attctcatta ttgatgatga cacagaacta acagatttat taacccaata tttggaacca
     8401 gaaggcttta atgtagtatg tgtgcatgat ggagaaaatg gcgttaaaag agctttaaat
     8461 caggtatttg atgccattat actcgacgtc atgctgccta agcttaatgg ttttgaagta
     8521 ttaaaggcaa taagagagca tttggaaaca cctgtattga tgttaactgc tcgtggtgat
     8581 gatatcgatc gcattgttgg gttagaaatt ggtgcagatg attacttacc taaaccttgt
     8641 aatccacgag agttagttgc acgtctgcgt gctattttaa gacgcactca aaaaatccca
     8701 actcccaaac ctattattga acaacataat atcattgtgg attgctccaa acgccacgtg
     8761 actatgggag gaaaattcct tgaattaacc aatgcggaat ttaatatttt ggagatgtta
     8821 atcaaatcac cagggcaagc attttccaaa gaggaactca ccgaatatgc tctgggaaga
     8881 aaatacactg cttatgaccg cagtatcgat gttcacatca gcaatttgcg aaataaatta
     8941 ggcgataatc ctcagggaga acctttagtc aaaactgtcc gcgggtttgg gtacatgttt
     9001 aatgcgtagt ctgtactgga aaatatttgt ttctttttgg ctggccacca ttttaattat
     9061 ttttactacg gcatgggtta ccagccaaat cgcacaaaaa tcatcacaac cagctcgaga
     9121 ggaaatgttc atggacagct atgccaatgc agctgtcgca acctatgagt caggccaaca
     9181 atcagcactt ttgaaatggc ttgataaaat aggcctttct cgtcatatga cactgtattt
     9241 actaaccagc accggcgaaa tcattggcgc acagaaacca cctgacgccg taaaaaaaat
     9301 ttcagaaaat ttaataaatg atgaactccc aagtgatggc atatttaaaa cagggaaatt
     9361 agtcgttagc catgagatcc tatcaacttc tggaaaatat taccggctag cagctgtcag
     9421 tgaaaagcca atctcttatt ttgtgcaaat tccatgggct ggactgacaa tacgtttaac
     9481 tctggctata ttcatcagtg ggcttatctg ctatttactc tcacgttatt taactcaacc
     9541 tttacgctca ttaggcatgg cagcaaaatc tatcgctaaa ggaaatttaa atacccgggt
     9601 tggtcgcttc agaggtcata gtaaagatga gattgcggag ttaagtaatg aattcgatcg
     9661 aatggccgag caattagaag cgcttgttca ttccaaagaa cgattaatac aggatatttc
     9721 ccacgaatta cgctctccat tggctcgact taaaatcgcc atagaattag ggaaaaagaa
     9781 aaccaagcat ttggctgata atgaatttgc ccgcatggaa attgaaacat ccagacttaa
     9841 tgatttgata ggcgaaattc tcgattttgc tcgtttagaa aaatcgacaa cagaacttca
     9901 tttatcccaa acaaatatcg cggatttgct tgcacaggtt ataagtgatg ccaattatga
     9961 gttcagtcac aattctataa gaatacaagc tggaataatt acaccttgtg aattaaatat
    10021 tgaccaacgc ctaatacaca gagctataga aaatattgtt cgtaatgcat cacactattc
    10081 tcctccagat gaaaaggtcc tgatttcatc aagctatgat aaaaacaaag atcaagttta
    10141 tattgatatt aatgataaag gacctggggt tcccgaagat caactggaaa aaatatttaa
    10201 tcctttctac cgcgtggata cctccagaac aaaaaaaacc ggtggctatg ggcttgggct
    10261 cgcaattgca gctagggctg tagcattaca ccaaggagaa attattgcta aaaatagaga
    10321 gcaaggtggt ttgcttgttc gtatcatttt acctgctgat gttaaatagg atttctatat
    10381 atcgagttcc tccttattga ctaaaattga tttttgactt gcatttttat tatctaattc
    10441 cgtatacata caaataatta tacattttgt tcataatccc agtttactgg ataaaaccta
    10501 tagatttaag taaaatgaat ctaaaaaata attggaatgc cattttgtga gcaatgatac
    10561 aaaattaaat aacgcaagta attatttctt tatatctgga tttgtaattt caaaaattaa
    10621 ataccttccc attgccgtgg tttcttccgc tctgaatcta atttcacttt tgttttacct
    10681 ggttggctat agcttgtggt ttattgcaag tcatttttac ccaggccaag ataaaaagat
    10741 gcaggagtgg tatgagtttg ctcaatttaa agaacaatat ctttatgccg cagctctagg
    10801 gctcgtcgca acatctatca gcatgatggc aatcttttcc ccaataatgc ttgttctttc
    10861 aggatggctg ttttttggca gtaatattgt ttggacaatt ggtgagtaca acaaattaaa
    10921 taatccaccc agccatgaag aaaatttttc caaagaccgc caaaatgcct atgtgtctta
    10981 tgctttatcc ataagtttaa taggattcat tacggcagtc tcaactactg tcgctttctt
    11041 tattccagta atgactatac ccttacttat agttacaact attgtatgta ttggagtggg
    11101 agcgttagcg tttgaatatt ggatcgaatc gaaatttggc gactctcagc ctgatatcac
    11161 tatcgatgaa agctacaaac aaatgagcaa tgatttggag aaaaaaatta ctttggagcc
    11221 caatcctacc ccagaacctt atcatggaac caggccactt cactttacgc cacatgatgt
    11281 aaaaatgaaa gaaccgtctc cagatcttca aatggattta tcctctcgta cttgtaaacc
    11341 acagtattaa tttagtatat tctgcacgcc aatgatgagg gtgtgacaat ggataaatta
    11401 acttcgaaca tgattaatga agcgcataag gtgctagtca acgcttatgc cccctattcc
    11461 aaattttcag tagctgcctg tatatgcact gataaaaata atttttatac aggggtcaat
    11521 gtagaaaata tttcttatgg gctaacaacc tgtgctgaag tttcagcgat ttgtaatatg
    11581 gttaccgcag gtgagcgaaa aattaaaagc attgtggtat tagctgcaaa caatttgcta
    11641 tgttctcctt gtggtgcatg ccgacaaaga attcatgagt tttcatcctc tgataccatg
    11701 gtacatctct gtaataaaga aacaatattt catagtatta ccattaatga attactaccc
    11761 ttggcttttg attttaaccc ttaatcggac gactttatga cgacttcaat atccactatc
    11821 agttacccac atttagcagc agaatatatt caaaaaacat gtccatcatt taaaccaaaa
    11881 gtgggtgtag tattaggctc agggttaggc caatttgccg aagagttaga agatacagtt
    11941 gctattgaat acgaaaaact cccaggattt cccagaacta cagtacaggg gcatggagga
    12001 aaattaattt taggatatta tggatctact gcggttattt gcttacaagg cagagcccat
    12061 acttacgaaa gcatggaaaa tcatgaagca gtaaaaacct atgtaagaac tttaaagcta
    12121 ttaggttgcc agtattttat agcaacaaat gcttctggtt cacttaaaga agaagttgga
    12181 ccgggtgaat taatgttaat taccgatcac atcaattttc aacccggaaa tcccctggtt
    12241 ggtccgaatg atgatgagtt tggacccagg ttttatcctt tagataatgc ttatgacatt
    12301 accatgcgca atgcattatt ggatattgct caaagacact cgattaaact acaccagggg
    12361 gtttatatct ctgtcttggg accaaactat gaaacagctg ctgaaattcg tgcctttaaa
    12421 ttgctgggag ctgatgcagt ggggatgtca actgtgcctg aagttttagt cgccaatcat
    12481 tgtggcttgc gtgtcgctgt tattgcaacc ataacaaatt atgcaactgg ccttgcaaaa
    12541 accagtcata gccatgaatc cgttgtcaaa atggcttcta gtgccgctga aaaacttaat
    12601 actttgatta aacagtatat tttggaatta tcgtgagttt ggataagcat tttaatgatc
    12661 tattggacaa gctcctgtca aaccaggata ttacatctat ttcgaatgct cagttgatgc
    12721 attgcattga tttgacacta ctagaggcgg atgcttctct tgaagcattg catcaattac
    12781 agagccaagc ctgtcaaaat aatgttgcag ctatttgcgt gctacccaaa catcttcatg
    12841 tatttaagcc taactccaaa attaaattag caactgttat caattttcct cttgggaatg
    12901 atgatttgct ggtttccctg gcagagatag ataatgccat tcaactgggt gctaatgaaa
    12961 tagattatgt actaccctat cagtattact taagaggaaa taagcaaaaa gcgttaaatc
    13021 attgtgatgt cattattcaa acctgcaaga aacatcaact taccttaaaa ataattttag
    13081 aaactggcct ttttccagat atggaaagca tttatcagct tagctcagaa atcattgctc
    13141 ttggtggacg gtttctcaaa acttcgactg gaaaaacccc tcagggtgca actctggcag
    13201 cagtatttgc tatagtcagt gctgttatgg attcaactta tgcatcctct tgtggaataa
    13261 aggtttcagg agggattaaa acgcctcttc aggctcaaca atatgctcaa ctggctgaat
    13321 tacttatggg caaagcgatt cataaggatt ggtttcgtat tggagccagt actctgcttg
    13381 agaaattatt gtcatgaacg cacagtctat gtgttaatat gtcagcatgc aaacagggag
    13441 agctcttgca atgaaattat ggataatgct atttaactgc tttattgtta cctttttaat
    13501 aacattctct cacgcaaaag cgccagtaaa taacaataaa tcatcttatt gcctccctaa
    13561 ccaaccatgc tggccaaccc ccaaagaatg ggagcaacta aacaaaaaaa taaatggcca
    13621 tttaataaaa gccaggaact atctgtcacc ttgcattgaa gatcccaaaa gtaatggttg
    13681 taaaaatacc ctggaggaaa taaaaaatcc cttttatttg gaaacacaac ctaccgcaac
    13741 ccagtccacc ggctggtttg aggcatggga ttcagaaata agcccataca tagttgcagc
    13801 aaaaacagcg caggaaattg ctcatgcagt aaaatttgct agaaaacatc atatcaaact
    13861 agttgtcaaa ggcactggtc atgactatct tggaagatcc aatgctcctg attctttgct
    13921 gatttggaca catcacatgc gagaaataca gttccagaaa catttcgtcc caaatggttg
    13981 ttctgcaaat cataagggtg ttcctgcggt aactgttgaa gcaggtacca gatggataga
    14041 ggcttataaa gaggttactg taaaccaggg gcgctatgtt cagggcggag gctgtacttc
    14101 cgttggagtt gctgggggat ttattcaagg tggaggattt gggagttttt ctaaaaaata
    14161 tggaaccggt gcagggggaa ttttagaagc agaagtaatt atcgctgatg gttcaatttt
    14221 gattgccaat gaatgccaaa accaggattt atattgggca ttgaaaggag gtggcggcgg
    14281 aacttatggt attgtaagta aagtcacatt ggaaacgcat gaattgccag caacatttgg
    14341 agcattaaca ggaagtatta cagcaaaaac agagcaggat tttaagaatc tcatagatta
    14401 ttttattatt ttttacagag aaagacttca taatgaacat tggggagaac aagtcatatt
    14461 aggccctgat aacaaaatag agctggcatt ggtttttcaa gggatgagca aaactcaggt
    14521 tgatagatta tggcaaccat ttaaaacttg gttatcccaa aaacctgaac attatcaatt
    14581 tacaataaac agcctggtac tacctgcaag aaaattctgg gattacgatt atctaagcaa
    14641 aaatctacct gatcatattg taattgataa gcgaaaaaat atcccttatc cagagttttg
    14701 gtggtctggt aatcagtcag aagtttctat ctatttaact cactaccaat ccggttattt
    14761 gccctatacc ctctttcaag aaaaattttc gaataaacta tctaacgctt tgtttgaagc
    14821 atctcgcctg accaaactgt ctttgcattt taacaaaggc ttggcagggg cgtctcagca
    14881 agcaatcgag agacaaagaa aaacatcaat gaacccttcc gtattggatg cagcagcctt
    14941 gataacaata attggggggc aacaatatgt ctttcccaag ttgcctgagc acaaacctga
    15001 tttagataaa gcacggtttt cagctaaaca ggcaaaaaaa gcaatgaagc taatatctgc
    15061 tctgaatcct gattcgggca cctatggaaa tgaggccgat tacaatttaa aaaattggca
    15121 aagggcttta tggggatcca attatgagaa attattacaa attaaacacc attatgatcc
    15181 cgaaaacttg tttaattgcc accattgtgt tggaagtgaa taaaaattaa ttaataattt
    15241 taagaatctc agttagaata actagtcata gtgaattaat tagcattgga gtttaaatta
    15301 tcatgaagac taaaatcata tttaaatcca tttcatcatt actattgggt ttgctacttg
    15361 taagttgtag caatgaactt cctcccggtg aatatgatgc aaatgagata ggcaaaataa
    15421 aaaaggttat tccgggtgtg attatttcca aacgaccagt taatttacat cgtcaaaacg
    15481 ctgatgccaa tctgcatgta aaaggcggtg ctgattctaa tttgatagat ggtggttata
    15541 atagaacaca tggtgtcgaa tacgtcatca agttaaactc tggcaccatt atttcagtgg
    15601 tacaagcaga agacttgaaa ttaaagaaca agcaaaaaat tctggtaatt tatggaagac
    15661 acactcgagt tgtagctgat aatggaagcg atgtatatta attagccttc ctgtttcaaa
    15721 atatgggttg gtgttggatt ttttcttcta aagcctgtct tatgaaggaa ttttccgaca
    15781 cccataatca tttggcacaa caaattttct attagggttg agaggacata ttatttaaat
    15841 caatgctaat ccaacggcaa accacattac taatccataa taaccactca ctaaaaatgc
    15901 tttaaaacaa tttttaggta ttcttgcata aattaacttt agttgataag tcagtattcc
    15961 agccgcagta caccataaca aataaaaaaa ccattctgcg ttcttattta ttgcccatac
    16021 caaccataaa gaatgcagaa aaatcagtaa caaagcaata attaatctat cataacttgc
    16081 aaaatatatt gcagtcgatt tgactcctat ttttaaatca tcggctttgt cagtcatcgc
    16141 atacatcgtg tcataagcga taatccagga aaaattaatt aaaaacaata caataaaatc
    16201 actatttaaa tttttccctg aggcaataaa tgccatagga atacccatgg aaaaagccaa
    16261 acctaatatc agttgtggag cattcaaaaa tcgtttacaa aatgggtata aaaaagtaat
    16321 tagcacggaa ataaccgcaa aataaaaaca attgattgga agttttaata ataaaagcaa
    16381 tgaggcacaa agtaatataa acaataaaat aaatgcctca gacaagctga cctctccagc
    16441 agtcaagggg cgaaattggg ttctggctac atgcctatca atatgcctgt cagcaatatc
    16501 gttaataaca caacctgcgg aacgcataaa tactgtacct aataaaaaaa tcataagcaa
    16561 atcaatagaa ggaaagccct gatttgccat ccaaagtgcc catgcggtgg gataccaaag
    16621 taataaaatt cctattggtt tatttaagcg taataatctg acataggcat tccagggaat
    16681 cataataaat tttccagctc tggtaacaag atttcaacca aataaaaaga actcttttcc
    16741 tgaaatacta gctccgtaaa cctgacccac atttgagagt attcagcgct aatgtatttt
    16801 ttaacccaat gaaactcaag atttaattga tctacaggat aacaaagtat tggtaaccgt
    16861 ctcacactac tttcatcaaa aatgagattt cgtattgatt cattttccaa acggttgaaa
    16921 aactctggct taagggcata acacgattgt gggataaccg atcgcgcgta ccaatagata
    16981 atcccttgat ttttcataac aatttcacgt tgaagaatgc ttttgtccgg tatacacaga
    17041 acatacttat cccaccaatt tgggatagac cagttctgag ataaacgttc cagctccgca
    17101 ttaccggtta ttgcttttag tttatctgtt aaggaatctt ggtattctaa ccaatttttg
    17161 agcaatcctg gccctttggt atcggcaatc agcagggatt cataattaat agacatacat
    17221 aaataatttg gaaaaaaatt tcataagata ttacaaaatt acctctttat gaacaaccat
    17281 tgcttataat ttaagataaa aacaaaatcc ttttattttg atcttgtgtt aattaccccc
    17341 taccctaacc ctgccaagcc tatttaaagt aaccacaact ttttcattgg ttcgcttatt
    17401 gttcaaaata aatttaccat tactcatcgc acgattcgga atattggata taataattct
    17461 atggtttgaa tctacgcctt tccaattaat attccaggaa ttagaactcc attgccattg
    17521 gtgaataagc tcagttttat tggttgtttg attaagttta gcaagcacca tccccctgga
    17581 ccaattttca ttagaaccaa aaggaagaag ataaatcgga tgccccaaat gaatagcctg
    17641 tattttggag tattgaatgg ctgtttttat actattaact aatgtttctc tctcattatt
    17701 ttgtattagt gaaaaaaatg accatgaacc taaaagcaat aatcctacaa gcaaagctaa
    17761 tgtaagcatc gtttcaagta gagtaaatcc tgttattttc atcaattgaa gccgcatatc
    17821 atctacaaaa ttttatgatg aagataagat gcgatcctaa gagcaatcgc tttgggtgat
    17881 atggctgctg cataggctaa aattttattt cttagccccg gaataatata caactttcct
    17941 ttttttaatc ctttataggc aattttagcg accttttctg gtgtcatcat tccaatatat
    18001 cccctctcca acatagaact ttccatacca gaagcatgga aaaaattggt ttttgttgct
    18061 ccaggacaaa gaatagacat agatacctta gtatctttaa gctcataagc aatagcatgg
    18121 gaaagcgttg ccacatatgc cttactcgca tagtaggctg ccataaaagg acccggttga
    18181 aatgctgctg ttgaagaaac ctgtaaaaca ctgccttcgt ttcgagccac aaaatcatga
    18241 agaaaacata gtgttaattc acttaaagaa atcatgttta tgttcattaa atcatcgagc
    18301 ttgttcatgc ccatctcaac aaaagaaccc atatagccta tgcccgcatt attaaccagg
    18361 cagttgactt ctacaccctg ctcttttaaa tcagtataca acttaaatgc gcttccaggt
    18421 ttacttaagt catgtacttt atattcaaca ttaacttgat attgtgtatt taattgcttt
    18481 gaaatgtgct ccagctgttc agaatctcta ccaataagta ttaaatcatg gccatgtgat
    18541 gcaaactcat acgccaaggc caaacctata cctctcgtgg ctcccgttat taaggtcatc
    18601 atgatgcact cctcctttta ctgtttgcac aggctccatt tggttacgta ggcaatccaa
    18661 tagccgagat acatcgtcat gcttttcctg ttttaacaaa ttcaagatcc gtatacattg
    18721 agggagacct aaatcccatt tgccaaaata atttgctttc ataagattaa tgtcgctata
    18781 caataaatgc ctaacctctt ttaatacatc ataaatttgt tggcatgatc tttctttgag
    18841 ctctttaatt aatttaacga tgatatgaat cataacatga gcatttcctg aaattttgtt
    18901 aaaatgctca agacaatcaa agggagaatg tatatttagt gaatgacgtg cacggtctaa
    18961 taggttctta tcaattttat ttattaataa atcagtagat agttttaaaa aaattggata
    19021 gagtgccagt aagacatcat ggtttccatt ttcaagttcc tggctgtgat tatgatgctg
    19081 acaaaccgca ttaccaaaaa aagttaaagt attgctttca ttacaagctg ttttattctt
    19141 ttcaggatta gatggtagag acgtattaaa ataaagtttt ctaagttcaa gggattgttt
    19201 cattaaagca gtacgattag gtaaggtaga ttgcacaatt agattattaa caacattaaa
    19261 ccattgttca tctcctctgt aacaacagta agcaagcaaa tctcccaaag aattgaaagt
    19321 agagtacatt atttccttat taacatagct gttgtttttt ctagtatatc ttacattaag
    19381 ataatccaga gttgattgat aaccattttg gattaattcc tcctggctct gcctctctac
    19441 attaaatcct gttgttgata ctttccctgt ttcaggaatt atagtttgaa gagcatattg
    19501 tcttaactta aggcggtctc tttcccatcc acttgcagga tc
//
LOCUS       DACTJE010000047        19403 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.047, whole genome shotgun sequence.
ACCESSION   DACTJE010000047 DACTJE010000000
VERSION     DACTJE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19403
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.047"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(3..1025)
                     /gene="aph(9)-Ia"
                     /locus_tag="I9760_001296"
     CDS             complement(3..1025)
                     /gene="aph(9)-Ia"
                     /locus_tag="I9760_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(9)-Ia"
                     /protein_id="HAT5043072.1"
                     /translation="MLRNNIPDQHLIELLKVYYGIDIHTVQLIVGGADMNAFGYKADS
                     ESNSYFVKLKYGHHDEINLSIIRLLHDSGVKEIVFPINTLEANLFQQVDHVKIIVYPF
                     IDAPNGFTKNLTEKQWKQLGKILRQIHGTSVPTSIQQRLRKETYSPKWREMVRSFYSQ
                     IRFDESDDQITTDFKSFFNQNIDSIHQLVNSSEELSKKIRPDFEKYVLCHSDIHAGNV
                     LVVDEESIYIIDWDEPMFAPKERDLMFIGGGIGNVWNKPHEIDCFYEGYGKTSIDKTI
                     LSYYRHERIVEDIAVYGHDLLSHDQNAQSRLESFKHFKSMFDPNDVIEIAFASYFNLL
                     FFRIIQ"
     gene            complement(1402..1782)
                     /locus_tag="I9760_001297"
     CDS             complement(1402..1782)
                     /locus_tag="I9760_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAT5043073.1"
                     /translation="MSKTYTEKLFSYGTLRYEKVQIANFGRKMQGQEDVLQGFKLTTI
                     EIKNSAVVATSGDNFHPIISYTGNPSDKVPGIVFTISKEELAQADKYEVSEYKRVNVK
                     LNSGVNAWVYINAQDLYGNNQAKS"
     gene            1871..2164
                     /locus_tag="I9760_001298"
     CDS             1871..2164
                     /locus_tag="I9760_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAT5043074.1"
                     /translation="MMSKFFNLKTGTLHSVESYAGAVKLPKGISQESIRINERILDND
                     GYPLMAPKLRRQNANQLRMFFSRESNTLLNGSYDLADRSNRDLTEVANIISYR"
     gene            2500..4296
                     /gene="lem10"
                     /locus_tag="I9760_001299"
     CDS             2500..4296
                     /gene="lem10"
                     /locus_tag="I9760_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem10"
                     /protein_id="HAT5043075.1"
                     /translation="MVTKIIWVSNNGKPNLKIEFVSEEEKSNFFKEVKKKASELGLNF
                     PLVQGSGNSLLIEASNYPINPCGCYISPGGKLAINFGKVELSHFILPKVGVKTEHAEI
                     FKDHNTIFFHKHKLPGVNSELTFIPTGTPVIVPVTKPATTSPAFFNPSKSGDSSISIS
                     AIGNVDSPMIRITFQNQTEREFFLNKITDKAKSLGVNISTHPFEIKEPNMVLIKPSKY
                     PDNKLGCYISKNKEIAINFGRTDFRDFVLSNLGVGSHLGTCPTKNETGNDTFYFHQEN
                     LSLNGPALSVNTKITKEIEKNDYLLSANGSKAIDFVWSNFLKHPYNPKLKQPDANVKA
                     AWEKHADWEHWSNMGQDWSLNTPSGLVPRPNHGIAHTLRVAQLVPVIAEFLKAYSGDP
                     KFQKLTQKEIQKAQYMMLFSVIGRENDMSWTDANHYQQAFKEAYNGQYSKHIYATFKE
                     NAQKGFLNHVVTNKSSLIPSLFSDESELQYWAEALDTGKPGISSASGILMALAHDLDL
                     MRCYDKGKFNSLKMKDLVARLGGNEDAAKKLADYAHDLIVATGDRCMGYGVTQDYNYS
                     LFGKCSLDPNECLKQLQSIPKPETTLANQYGI"
     gene            4534..7215
                     /gene="pepN"
                     /locus_tag="I9760_001300"
     CDS             4534..7215
                     /gene="pepN"
                     /locus_tag="I9760_001300"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAT5043076.1"
                     /translation="MKTDQSKVKKLSDYKSLDYFVIHVDLQIDLSKKPVESKARLTVV
                     PNLNVDSHSNDLVLDGENMTLVSLQMNDNLLKENEYELTKDSLIIKNIPQNTPFTIEM
                     TSLLGENTDLFGLYETEGVALVKAESEGLRRVFYLPDRPDNLATYKTTIIANQEDYPV
                     LLSNGVLIEKKELPLGLHSVTWLDDVPKPSYLFALVAGNLQRSVTYYQTKSGRELPIE
                     FYVPPSATSKCDFAKEVLKEAMAWDERTFNLECALRQHMVAGVDKYASGASEPTGLNL
                     FNTENLFASPETKTDLGILRVLEVVAHEFFHYWSGDRVTIRDWFNLPLKEGLTTFRAA
                     MFREELFGTDLIRLLDGKNLDERAPRQSAYTAVRSLYTAAAYEKSADIFRMMMLFIGK
                     EPFIEAVAKFFKDNDGGAVTLEDFIESISNSSGKDLRSFLSWFTESGIPELIVTDELN
                     PDTKQYFLKIKTVNGRNRPIPILMGLLDSSGAEIVADKLLIVDQEEIEFQFENIQTRP
                     IPSLLRSFSAPVHMKYEYSYQDLLLLMQFDTNLYNRCEAAKQLISALINDFCIGKKIE
                     LSPQFFAVYKALLSDNSLNEWMLAELITLPSLEELIENQDKPDFEKLNEGRQLIQNAL
                     ANELKTDFYNLLFRIQISGDDDKQKLKGFDLKQAGLRRLKSVCFSYLLNVDFEKTKEK
                     LILQFEDALGKNMTETALALSMLCEINCEEADVALEDYYHYWKNDPGAVNNWFSIQAL
                     AHSPDVIERVKKLMRHGDFDLSNPNKVYALLGSFIKNPFGFHSVTGEGYQLVADAIFD
                     LDKINPTLAANLTEKFTYWDKYDVNRQAMMISTLKIIYSNATSSDVRTMAKKGLDKVK
                     EDLPLPIHLTFHGGSTMQDRTAQLIADGNKENAYQLH"
     gene            complement(7357..8280)
                     /locus_tag="I9760_001301"
     CDS             complement(7357..8280)
                     /locus_tag="I9760_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT5043077.1"
                     /translation="MSSKNTHSTLLNKQEINWILEQRITRLTDNKEVLIYPMGAERYL
                     SSALSGHEEERYNLHSEIKEKNVLIIPGYGNSAFLFAQAGAKSILVYDKDPVTIAWVK
                     AFKKYYHYREYNNNGKPYPSIGELLTALTKWYPPLLKLPSGVIKNSVFWIINPKLLRK
                     SYIYYMLKLTRQAVQLRVQNEFEFDHHIEFYAGELKHLIKNHPSKTFDTVFVPYLLGV
                     QNGIEKEKDIVSFIKQLIELVPYGHILVTPSKSTKEFHLVGQSYFCTTAYSNIQSIPD
                     LKPYVIAEDSIWFKTQGLAVFGIKPDAEKRL"
     gene            complement(8367..8900)
                     /locus_tag="I9760_001302"
     CDS             complement(8367..8900)
                     /locus_tag="I9760_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043078.1"
                     /translation="MSFDFIKSIWIAALIGITPTLSYSESNIQYQETLQSHCFETWMK
                     KMDPSIDKETYKQFGDKYCRCISTKNLDNKTGVQKAIRNCMPQTILHDAMDELEEEVT
                     LSEVTTQTIEQYCLNRWSLIYPNQSDEDKKTIQAYCACVKTKLLSFIEQIEKISNREY
                     NEAINDINTICSENLNQ"
     gene            complement(8958..9599)
                     /locus_tag="I9760_001303"
     CDS             complement(8958..9599)
                     /locus_tag="I9760_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAT5043079.1"
                     /translation="MKTLVITGISRGIGLEIARIFLDKDWTVIGTSTSGYSPIKHKNL
                     RTYKLDLAISEHIDAFAKNLPKIDVLINNAAVLLEEWNEEKINMSLLKKTFAINLFGT
                     VELTEKCIPKFNDSAQIINISSGWGAFSSNNSPFQPHYKMSKVSLNMYTKLLAERLPQ
                     ITVSSFDPGWVKTNMGTQNAKKLPSETALEIYELISMKKKSGYFWFNGKPRDW"
     gene            complement(10159..10623)
                     /gene="rimI"
                     /locus_tag="I9760_001304"
     CDS             complement(10159..10623)
                     /gene="rimI"
                     /locus_tag="I9760_001304"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAT5043080.1"
                     /translation="MILNIRRMKDSDIENVYSIETNVHIAPWSKDILRDCVLVGYDCR
                     VLEINNGDSSILAGYIISRISNNSCHILNLCIAKPLQSKGLGRKLLQTVLYSLSKYTQ
                     TESVILEVRPSNSAALHLYETMGFEKVEIKKDYYKDKNSVEDAILLKKLLHH"
     gene            complement(10959..12383)
                     /gene="lpdA"
                     /locus_tag="I9760_001305"
     CDS             complement(10959..12383)
                     /gene="lpdA"
                     /locus_tag="I9760_001305"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAT5043081.1"
                     /translation="MANKIKTDVVVLGSGPGGYTAAFRAADLDKKVVLVERFDSLGGV
                     CLNVGCIPSKALLHIAKVVDEAHEMSEQGVAFGKPKFDNKKLVAWKNSVVAKLTGGLK
                     ALAKQRKVEVITGTGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIKLPFIPEDKR
                     IFSSTGALELADIKGDLLVLGGGIIGLEMATVYSSLGVNVTVVEFMDQLIPNADADLV
                     NILQKRMTKKGVKFLLKTKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVSVGRKPN
                     GGAINAEKAGIKVDERGFIPVDNQLRTNVPHIFAIGDVNGQPMLAHKAIPEGKVAAEV
                     IAGKKHYFEPKCIPSVAYTDPELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMGR
                     EEGMTKLLFCPETNRILGAGIVGVNAGDLIAETALAIEMCCDVEDIALTIHPHPTLSE
                     TIAQSAEAFEGTITDLYLPKKKKK"
     gene            complement(12472..14106)
                     /gene="aceF"
                     /locus_tag="I9760_001306"
     CDS             complement(12472..14106)
                     /gene="aceF"
                     /locus_tag="I9760_001306"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAT5043082.1"
                     /translation="MTKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEK
                     ASMDIPSPISGTVTQILVKVGDKVSEGDLIVKAKSDTTTNISSSQEQKTEPEKQNLQT
                     RAEEQSVDTKATASTPSSKDIEISIPDIGGANDVDVIDILVKPGMEVEKDQALITLEG
                     DKATMDIPSPYAGKVIEMKIKLGDKVSQGTPILTLKTLGKSETPEIEKSQIKNISEQS
                     IKEIEKPYEELKSEAISINNLEIAESKSILISAGPAVRRLAREFGVDLSLVQGSGRKS
                     RITKEDLQNYIKVRLSEKTTSGGFSLPSNPAIDFSKFGSIETKPLNKIKKLTGTNVHR
                     SWITIPHVTQFDEADITDLEAFRKSESESAKNQDYKLTLLAFVCSVVCKALHAYPQFN
                     ASLDTSGENLIYKKYYNIGIAVDTPNGLVVPVIKNVDKLSVIDIAKEMSRLSTKAREK
                     GLTPIDMSGGCFTISSLGGIGGTAFTPIVNSPEVAILGLSRSIIKPIYDNKEFKPRLM
                     LPISLSYDHRVIDGAEAARFTRFLCDCLGDIRRVLL"
     gene            complement(14164..16827)
                     /gene="aceE"
                     /locus_tag="I9760_001307"
     CDS             complement(14164..16827)
                     /gene="aceE"
                     /locus_tag="I9760_001307"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAT5043083.1"
                     /translation="MTNETNLDLDPIETREWLDALQAVLFNDGPERTAFLLQQLLNKA
                     NAEGVKLTSSINTPYRNTIKPHEEKQMPPDEGIGKRINALIRWNAVAMVLRAGKYAPE
                     LGGHIASYASASTLYETGFNYFFKGQKGENGGDLLYIQGHSAPGIYARAFLEGRLTEK
                     QLEKFRQEVEVDGLSSYPHPWLMNDFWQFPTVSMGLGPLQAIYQARFLKYLENRGLIK
                     AEGRKVWAFLGDGEMDEPESVGALSIAAREKLDNLIFVVNCNLQRLDGPVRGNGKIIQ
                     ELEGLFRGAGWNVIKVIWGGRWDPLFARDNQGWLQKRMEECLDGDYQSYKANDGSYVR
                     QHFFNQYPELKKMVENMSDEEIWRLNRGGHDPQKVYAAYARAVEHKGTPTVILAKTIK
                     GYGMGAAGEGQNITHQQKKMTIDQLKAFRDRFNIPISDDKIADIPFYKPDDDSPEIKY
                     IKKQREALGGYLPHRSTEVEQLKIPDLGEFSSITKGLGDREISTTMAFVRILSVLLKN
                     KDINSRIVPIVPDECRTFGMEGLFRQIGIYSPVGQLYTPVDHEQVMYYREAVDGQILE
                     EGINEAGAFCSWIAAATSYSSNKLAMIPFYIYYSMFGFQRIGDLAWAAGDMQARGFLL
                     GGTAGRTTLAGEGLQHQDGHSHVLASTIPNCISYDPTYAYELAVIIQNGLHRMYEKQE
                     NVFYYITIMNENYSHPDMPAGVEEGIIKGMYLLKENKKKSKNHVQLMGCGTILREVIK
                     AAEMLEEDFSITSDIWSVTSFNELRKEGLAVERYNNMHPQNKPQESYVTSQLKGRRGP
                     VIASTDYMRIYADQIRPFVPNRYITLGTDGYGRSDTRTQLRHFFEVDAKFIVLAALNA
                     LVAEGSLDKTKIVDAMKRYNINQDKLDPMTH"
     gene            complement(16855..17244)
                     /locus_tag="I9760_001308"
     CDS             complement(16855..17244)
                     /locus_tag="I9760_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAT5043084.1"
                     /translation="MNTAFRLLFCLIILELSACATLKSKITHHKTLSQCQQTCFQQLN
                     YCKQNCTDNCRDCSTKVNHFAKENYLEYLHEVKIQGGYITRGLQSYRDPLQCRKVTCN
                     CSADFNACNQGCSGVIQKRLQPVPYCS"
     gene            complement(17283..18035)
                     /locus_tag="I9760_001309"
     CDS             complement(17283..18035)
                     /locus_tag="I9760_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043085.1"
                     /translation="MKLLVIVLCLLSERFLIHSVSYQRFSWFNNYCLFLKKTIDKNKC
                     FSNPWATLIAIILPIVFLTFLVYFSLQSILFGLFGLILSLFIFYYCLGPQNAFYPILK
                     KQANQTETDAIGEYFAEVNSQLFAVVFWYIIAGPIAALTYRLITLCKEINFISTQANQ
                     ITNILEWIPARITALLFLLVGNFQRGFHLFAQYVLTYPDSNDKILRGCGLQAVRINDT
                     EEVPMAAAENLVEHATIVLLVFIALFTLVAWL"
     gene            complement(18040..19212)
                     /locus_tag="I9760_001310"
     CDS             complement(18040..19212)
                     /locus_tag="I9760_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003637008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAT5043086.1"
                     /translation="MHDGSVFYTIFLIFAGAAFFSTLVLYTKQSLLVAYITLGAILGP
                     WGFKLVSDVTVVKQIGDIGIVFLLFLLGLHLQPQNLIHMLRKVTWIAIVSSILFAALA
                     YGIGRWFGLTVTESLILGGAMMFSSTIIGLKLLPTTILHHQHTGEVMISVLLMQDVIA
                     IVLLILLNSAHQDGNLSISDFILVIIALPALCLFSFLFERYILIKLLARFDRTQEYVF
                     LLSIGWCLGISVLAQTIGLSEDIGAFIAGVALASSPISLFIAESLKPLRDFFLVMFFF
                     SIGATFNFGFGAQVVIPAVILATLILIIKPLLFYILLARSGEKKQVAKEVGIRLGQGS
                     EFSLLVASIALSTNLISEVASNLIQATTILTFIVSSYLVVLKYPTPIALSEKMRKD"
BASE COUNT         6028 a         3530 c         3408 g         6437 t
ORIGIN      
        1 atctattgaa taatgcggaa aaataagaga ttaaaatatg atgcaaaagc tatttcaata
       61 acatcatttg gatcaaacat cgacttaaaa tgtttaaaac tttcaagtct ggactgagca
      121 ttttgatcat gtgaaagcaa gtcatgtcca tacacggcta tatcttcaac aattcgctca
      181 tgtctgtaat aagataaaat ggttttatcg atgcttgttt taccataacc ttcataaaaa
      241 caatcaattt catgaggttt attccataca ttaccaatgc ctccgccaat gaacatcaaa
      301 tcacgttctt ttggtgcgaa catgggctca tcccaatcaa taatgtaaat ggactcctca
      361 tcaacgacca acacattgcc tgcatgtatg tcagaatgac aaagtacata tttttcgaaa
      421 tcaggtcgaa tttttttaga cagttcttct gaagaattga ctaattgatg gatagagtcg
      481 atattttgat taaaaaaaga tttgaagtca gtggtaattt gatcatcaga ctcatcaaat
      541 ctaatttgac tgtaaaaaga cctaaccatt tcacgccatt taggcgagta agtttctttt
      601 cttaatcgct gttgtatcga ggtgggaaca gaggttccat ggatttgtct taatattttc
      661 ccaagctgtt tccactgttt ttctgttaaa tttttggtga aaccattggg cgcatcaata
      721 aatggataca caattatttt aacatgatcc acttgctgaa ataagtttgc ttcgagtgtg
      781 ttgataggaa aaacaatttc ttttactcca gagtcatgca agagacggat gattgataaa
      841 ttaatttcat catgatggcc gtattttagc tttacgaaat aagaattgga ttctgaatct
      901 gctttatatc caaaggcatt catatcagca ccgcccacga tgagttgtac agtatgaata
      961 tcaattccat aatagacttt caaaagctca atgaggtgtt gatccggaat gttatttctt
     1021 agcataagag ttaattacta tttggaaatt atcataatgg taaagcaaat aatataattg
     1081 gaatactatt attactataa tattaagtct gattatcaaa ataatcagac ttaattggca
     1141 tgtaatgaac cctattcttc ttaggtataa tgaggtattt gtttaccctc tatcaaagtt
     1201 tgaattggag tacttccaca acacatttta ccttgatgag ttcgttgatt attgatgtgt
     1261 attttttagt ttttaaattg tataacgtat gggcattaca ttcgttttat aacttggcaa
     1321 taaagcattt cctacaaatt aataatagaa ttacccattt gagtctctat atatttgttc
     1381 tgaactaata tctattgcta gttagctttt tgcttggtta ttaccataga gatcttgagc
     1441 attgatataa acccatgcat tcacccctga gttcaatttg acattcacac gtttatattc
     1501 agagacttca tatttatcag cttgtgctaa ttcttctttg ctgatagtaa atacgatacc
     1561 tggaactttg tctgagggat tgcccgtata cgaaataatg ggatgaaaat tatcgccact
     1621 tgtcgcaaca acggctgaat ttttaatttc aatcgtggtt aatttgaatc cttgcaatac
     1681 atcttcttgg ccttgcattt ttctaccaaa attagcaatt tgtacttttt catagcgtaa
     1741 ggtcccgtat gaaaataact tttctgtgta cgttttgctc ataaaatcaa ccctcataaa
     1801 ataaaagtag tgaattttaa tcattttctt attattatca aagtgagatt tataaaacca
     1861 ataggagcaa atgatgtcaa agttttttaa tttaaagaca ggtaccttgc attctgtaga
     1921 atcctatgcc ggcgcagtca aattgcctaa aggcataagt caggagtcaa taagaataaa
     1981 tgagcggatt ctggataatg acggctaccc tttgatggca cctaagttaa gacgtcaaaa
     2041 tgcaaatcag ttaagaatgt ttttttccag agaaagtaat actcttttaa atggatcata
     2101 tgatcttgct gatcgttcaa acagggatct gacggaagtt gccaatataa tctcttatcg
     2161 ctaaatataa tcaggtattt gccagaataa taatcaggga aatacctgac aaaccagttt
     2221 ttcactgaat gtgtttggtt acttgctgat tattcttaaa gtcccatagc ataaacaaca
     2281 agctttgaat gattcatcct gatgatttta tggttagttg tatggctcca aatcgggtga
     2341 cttgttcatg tgcttcaatg taaagctaag attacttatt aggcatagcc gttaaatcat
     2401 ctaacctgat tttatgtcat tatccaaaac catataacaa attaatagac aaaatgtact
     2461 ataattctca aatattcctg ggaatagtag aggccaatta tggttacgaa aataatttgg
     2521 gtttctaata atggtaaacc taatttaaaa attgaatttg ttagtgagga agaaaaaagt
     2581 aattttttca aggaggtaaa gaaaaaagca agcgaattag gactaaattt tccattagtg
     2641 caaggctctg gaaattctct tctgatagaa gcttcaaatt acccaattaa tccttgtggt
     2701 tgttatatat ccccaggtgg gaaattagct attaattttg gaaaagtgga gttgagtcat
     2761 tttatattac ctaaagtagg cgtgaaaaca gaacatgccg agattttcaa ggaccacaat
     2821 actattttct tccataaaca taaacttcca ggtgtgaatt ctgaattaac gttcataccg
     2881 actggcacac ctgttattgt tcccgtaaca aaaccggcga ctacttctcc agcatttttt
     2941 aatccttcaa agtcagggga ttcttcgatt tctattagtg cgataggaaa cgttgatagt
     3001 ccgatgataa gaattacttt tcaaaatcaa acggagcgag agttttttct aaataaaata
     3061 acagataaag caaaaagttt aggagtcaat atctcaactc atccttttga aatcaaagaa
     3121 cctaatatgg tacttattaa accttctaaa tacccggaca ataaattagg ttgctatatt
     3181 tcaaagaata aggaaattgc aattaatttt ggaaggacag attttagaga ctttgtgttg
     3241 tctaatctcg gagtaggctc tcatttaggg acctgcccaa caaaaaatga aacaggtaac
     3301 gacactttct attttcatca ggagaatctt tctttaaatg gccctgcatt atcagtaaat
     3361 accaaaataa ccaaagaaat agagaaaaat gattatctac tatctgctaa tggctccaag
     3421 gccattgatt ttgtttggag taatttttta aaacatcctt ataatcctaa actgaagcag
     3481 ccagatgcta atgtaaaagc agcttgggaa aagcatgctg attgggaaca ttggtccaat
     3541 atgggacagg attggtcatt aaatactccc tcgggtttag ttcccaggcc aaatcatgga
     3601 attgctcata cgttacgagt cgcacagctt gtaccagtca ttgctgaatt tttgaaagca
     3661 tattcaggtg atccaaaatt ccaaaaatta acgcaaaaag aaatacaaaa agcacaatac
     3721 atgatgttat tttcagtaat tgggcgtgaa aacgatatga gttggacaga tgccaatcat
     3781 taccagcagg catttaagga agcatacaat ggacagtatt ccaagcacat ctatgctact
     3841 ttcaaagaaa atgctcaaaa aggtttttta aatcatgtgg ttaccaataa atcgtcatta
     3901 attccttcac tattttccga tgaaagcgag cttcaatatt gggctgaagc tttggacacc
     3961 ggtaagcccg gaatttcttc tgcctcgggc atacttatgg ctctggctca tgatttggac
     4021 ctcatgagat gctatgacaa aggaaaattt aatagtctga agatgaagga tttagttgca
     4081 aggcttggag gaaatgaaga tgcagcaaag aaactggctg attatgctca tgatctgatt
     4141 gtagcgactg gggatcgatg catgggttat ggggtaactc aggattataa ttactctctt
     4201 tttggcaaat gcagccttga tcccaatgag tgtctaaagc agcttcaaag tattcctaaa
     4261 ccagagacaa ccttggcaaa tcaatatggt atttgagatt aaatctttta tactatggct
     4321 gtagccagtt taaatcgtac ggttatttaa ttagcatcct ttgaatgcaa agggtgcttc
     4381 tattgctcta acaaataagc actagctcga ttgaaattac tatcttgatc ttatattgtg
     4441 acttcactga actgaaagcg gataacatgg tttttgatca gttgaaataa ggaatattaa
     4501 tttttagttt ttttggtaaa acagggagta tttatgaaaa cagatcaatc caaggtaaaa
     4561 aagttaagcg attataagag ccttgattat tttgtaattc acgttgattt gcaaattgat
     4621 ctcagcaaaa aaccagttga atccaaagcc cgtcttacag tagttcccaa cctgaatgtt
     4681 gattcacatt caaacgattt ggtattggat ggtgagaata tgacattagt atcgttacaa
     4741 atgaacgata atttattgaa agaaaatgaa tatgagctaa ctaaagattc tttaataatt
     4801 aaaaacatac ctcaaaatac cccctttact atagaaatga cctctctgct cggtgaaaat
     4861 accgatttgt ttgggttgta tgaaacagag ggcgtggcct tagtcaaggc agagtctgag
     4921 ggattacgcc gagtatttta tttgccagac agacctgata atctcgcgac ttataaaacc
     4981 actatcatcg caaatcagga agattatcct gttctacttt caaatggtgt gctaattgag
     5041 aaaaaagaac ttcctttggg tctgcattct gtaacctggc tggatgatgt cccaaagcca
     5101 agttatttat ttgctttggt cgcaggcaat ttacagcgat cagtcactta ttatcaaaca
     5161 aaatctggcc gtgaattacc catcgaattc tatgttccac catcagccac atcaaaatgt
     5221 gattttgcaa aagaagtatt gaaagaagca atggcatggg atgaacgtac ttttaattta
     5281 gaatgtgcct tgagacaaca tatggtagcc ggggttgata aatatgcgtc aggtgcttcc
     5341 gagccaaccg gattaaattt atttaataca gaaaatctat ttgcaagccc tgaaactaaa
     5401 accgatttag gcattttacg cgtgttggaa gtggttgccc atgagttttt tcattattgg
     5461 tcaggagatc gagtcactat aagagattgg tttaatttac cattaaagga aggtcttact
     5521 acgtttagag ctgctatgtt cagagaggag ctgtttggta ctgatctgat aagattactt
     5581 gatggcaaga atttggatga aagagcacca cgacaaagtg cctacacagc agttagaagc
     5641 ctatatactg ctgcagccta tgaaaaaagt gctgatattt ttcgtatgat gatgttgttt
     5701 ataggtaaag aaccctttat tgaagcagta gctaagtttt ttaaggataa tgatggaggt
     5761 gcagttactc tggaggattt tattgaatca attagcaact cttctggtaa agatcttcgt
     5821 tcttttttgt cctggtttac tgaatctgga attcctgaat tgatagtaac agatgaattg
     5881 aatccagata caaagcaata ttttttaaaa attaaaacag tcaatgggag aaatcgcccc
     5941 attcctatcc ttatgggttt gctcgatagt tcgggggcag aaattgtagc tgataaatta
     6001 ttaatcgttg atcaggagga aatcgagttt caatttgaaa atatccagac ccgccctatt
     6061 ccatcgttat tacgtagttt ttctgcccct gtacacatga aatatgaata ttcttatcaa
     6121 gatttattat tgttgatgca attcgacact aatttgtata atcgttgtga ggcagcaaag
     6181 caacttattt cagctttaat taacgatttt tgtataggga aaaaaataga attatctcct
     6241 cagttcttcg ctgtttacaa ggcattactt tctgataact ctttaaatga atggatgcta
     6301 gctgaattaa ttaccttacc gtctttagaa gagttgattg aaaatcagga taagcctgat
     6361 tttgaaaaat taaatgaggg gcgtcaatta atacaaaatg cccttgcaaa tgaattgaaa
     6421 acggattttt ataatctcct ttttagaata caaatatctg gggatgatga taaacaaaaa
     6481 ttaaaaggtt ttgatttgaa gcaagcggga ttgcggcgtt taaaatccgt atgtttttct
     6541 tatttgctaa atgttgattt tgaaaaaaca aaggagaaac tgattcttca atttgaggat
     6601 gctttaggca aaaatatgac agagactgct ttggctttaa gcatgttatg cgaaataaat
     6661 tgtgaggaag cggatgttgc attagaggat tattatcatt attggaaaaa tgatccgggc
     6721 gccgtaaata actggttcag tattcaagca ttagcgcact cccctgatgt gatagagcgg
     6781 gtaaaaaaat taatgcgtca tggtgatttt gatttgtcta atcctaacaa ggtgtatgct
     6841 ctgctaggat catttataaa aaacccattt ggatttcatt ctgttacagg tgaaggttac
     6901 caactggttg ctgatgccat ttttgacttg gataaaatta atccaacttt ggctgccaat
     6961 ctaactgaaa aatttactta ttgggataaa tacgatgtaa ataggcaagc tatgatgatt
     7021 agcactttaa aaataatcta ctctaatgct acttcatctg atgtgagaac aatggcaaaa
     7081 aagggattgg ataaggtaaa agaagatctc ccattaccaa tccatttgac ttttcatggg
     7141 ggatcaacga tgcaggatag aacagctcaa ttaattgctg atggaaataa ggaaaatgct
     7201 tatcaattgc attgatttct tatttcatga cctgatttgg aatagagtga gatattgata
     7261 ttgcctaaaa cttggggggg ggggggtgaa tactcccctt tcttgataaa gaatgagaaa
     7321 gcacgttttc taatacaata aatcatagtc ttattttcac aaacgcttct ctgcatctgg
     7381 ttttatacca aatactgcaa gtccttgagt tttaaaccaa atactgtctt ctgcaatgac
     7441 ataaggtttt aaatcaggaa tggactgtat attagaataa gctgttgtac aaaagtaact
     7501 ttgcccaacc agatggaatt ctttagtgct ttttgaaggg gtcactaaaa tatgaccata
     7561 gggtacaagc tctattaatt gcttaataaa actaacaata tctttctctt tttctattcc
     7621 attttgtaca cctaagagat aaggaacaaa tactgtatca aatgtttttg acggatgatt
     7681 ttttatcaga tgttttaatt cacccgcata aaactctata tgatgatcga actcaaattc
     7741 attttgaact cttaattgca cagcttgcct ggtgagtttc agcatataat aaatatatga
     7801 ttttcttagt aatttagggt taattatcca aaatacagag tttttaatca ctcctgaagg
     7861 tagtttaagt agaggaggat accatttggt gagagctgtc agaagttcac ctattgaagg
     7921 atagggtttc ccattattat tgtattctct gtaatggtaa tattttttaa atgcttttac
     7981 ccatgcaata gtcacgggat ctttatcata aacaagaata gatttggccc ctgcttgagc
     8041 aaataaaaaa gcgctattac catatccagg gattattagg acattttttt ctttgatttc
     8101 actgtgtaaa ttataccttt cttcttcatg accagataaa gcagatgaca aatagcgttc
     8161 tgctcccatt ggataaatta aaacttcttt attgtcagtc agtcttgtaa tacgctgctc
     8221 taaaatccag tttatttctt gcttattgag taaagtgcta tgggtatttt tacttgacat
     8281 gaataggcct ttcataaaag gattagaact agctggtgct caatgtgagt attaatttag
     8341 tcgacattta attacattat actatgttat tgatttaaat tttccgagca aatcgtatta
     8401 atatcattaa tggcttcatt gtactccctg tttgatattt tttctatttg ctctataaaa
     8461 cttaataatt ttgttttgac acacgcacaa taagcctgaa ttgttttttt atcttcatca
     8521 ctttggttgg gataaataag gctccatcta ttaagacaat attgttcgat tgtttgtgtc
     8581 gttacttctg ataaggtcac ttcttcctcc agctcatcca tggcatcatg taaaattgtt
     8641 tgtggcatgc aatttctaat tgctttttga acccctgttt tattatcgag atttttagtt
     8701 gaaatgcatc gacaatattt atctccaaat tgtttatatg tttctttgtc aattgatgga
     8761 tccatttttt tcatccaggt ttcaaagcaa tgtgattgta gtgtttcttg atactgaata
     8821 ttggactctg aataagacaa ggttggtgtt atacctataa gagctgcaat ccatattgat
     8881 tttataaaat cgaaactcac gtttgtcact cctttgatgc tttattttat agttgagaat
     8941 aactgaaatt taactggcta ccaatccctt ggttttccat taaaccaaaa atagccgctt
     9001 tttttcttca tactaatcag ctcatagatt tccagtgccg tttcactggg aagttttttt
     9061 gcattttgtg ttcccatgtt tgttttaacc caacctggat caaaactgga tacagttatt
     9121 tggggaagtc tttccgcaag caatttagta tacatattca aactgacttt tgacatttta
     9181 taatgtggtt gaaaggggga attattggaa ctaaatgctc cccaccctga agagatatta
     9241 ataatttgtg cactatcatt gaacttgggg atacattttt cagttaactc aactgtacca
     9301 aacaggttaa ttgcaaaggt ttttttcaat aaggacatat taattttttc ttcattccac
     9361 tcttcaagca acacggcggc attattaatc agtacatcaa ttttaggtaa attttttgca
     9421 aatgcatcga tgtgctccga aatggctagg tctaacttgt atgtgcgtag gtttttatgt
     9481 tttataggag aatatccgct ggtggaagta ccaataaccg tccaatcttt gtcaaggaaa
     9541 attctagcaa tttccaggcc tatacctcta ctgattcctg ttatgacgag agtcttcaat
     9601 ttaaatcctc tatccgtggc ttgtaaaatc aaaataattg atcttttaac tttttgcaaa
     9661 actcaatctc ttagttaaaa aaattttata attcaatcat ttggtttatc taaattccct
     9721 cctaaaaaac attttttacc tggatcttga gatttttctt aagccccaag ggaagatgcg
     9781 tcctttaatg tttatggaat atctcataca agcataagat tcattgcgtt gattatttaa
     9841 aattgctggt taatgccagt acaacatttt ttgataccat tgattcaagg aattcacagt
     9901 actatacaca agtttatcca cagattttgt ggataaaaat tttataatta cattaaataa
     9961 cctttgtgaa ataagacaga ttaaccatca actaatgggt taggaaaata aaatattaga
    10021 atatgaaaaa agttatccac atgtgaataa atcacataga agttttgaaa actcctacat
    10081 gataacaaag aaaaaatgaa aaaacagtct tgacttaaaa ttttcctata aaatttttaa
    10141 tagtaagaat attttatttt agtgatgaag tagttttttt aagaggattg cgtcttctac
    10201 actgttttta tctttataat agtctttttt aatttctact ttttcaaatc ccattgtttc
    10261 gtaaagatgt aatgcagcgc tgttacttgg ccttacctct aatataactg attccgtttg
    10321 agtatatttt gataaagaat aaagaactgt ttgtaatagt tttctcccca aaccttttga
    10381 ttgtaatggt ttggcaatgc acaaatttaa tatatgacaa ctattattgc ttattctgga
    10441 tatgatatat ccagcaagga tcgagctatc tccattgttg atttccagaa ctctgcaatc
    10501 ataaccaaca agcacgcaat ctcttagtat gtctttactc cagggggcaa tatgaacatt
    10561 tgtttcgata gaatagacat tttcaatatc actatctttc attctacgaa tgtttaatat
    10621 cattcattac tcctgggaat aatcatactt aatttatgat gaattaaata ttagcaaatg
    10681 tgttatcaaa cattactaat ccaaaatact gcacttaata attctttagt cttttattta
    10741 ggccctttgc atgactgtcc tttcattttt ttgctgtgct gcatatattt ttgtgggatt
    10801 tatgtaaact ctaccccgca aaaagtttgt atcttgtcca aaataaaata atcagcccat
    10861 gcaagaggtc tatatataat tatatacata taataagcat ccctttgctt ttatgagcaa
    10921 aggaaataca atgaggtata attggctacc tgtaattatt attttttctt ctttttaggc
    10981 aaatacagat ccgttatagt gccttcaaat gcttctgctg actgagctat ggtttctgat
    11041 agagtcgggt gaggatgaat agtaagagcg atgtcttcaa catcacaaca catttcaatg
    11101 gctaaggcag tttctgcaat taaatcgccg gcattaaccc caacaatacc tgctcccagg
    11161 atacggttag tttctgggca gaatagtaat ttggtcattc cctcttctcg ccccatgctg
    11221 agtgcacgac cgctagcagc ccatggaaag gaggctttct catagtttat tcctttctct
    11281 tttgcttctt tttctgttag accagcccaa gctaattctg gatctgtata agcaacacta
    11341 ggtatacatt ttggctcaaa ataatgtttt ttccctgcaa tgacttctgc tgcaactttc
    11401 ccttcaggga ttgctttatg tgcgagcatt ggttgcccat tgacatcgcc aatagcaaaa
    11461 atatgaggaa cattcgttct taattgatta tcaactggaa taaagccacg ttcatctact
    11521 ttgatccctg ctttttcagc gttgattgct ccaccgttag gtttcctgcc tactgaaaca
    11581 agtacttgct gaaagcaaag aggcttatca gttgcatgtt ctccttccat tgaaacataa
    11641 ataccatctt ttttggcttc aactgcagtt actttcgttt ttaatagaaa tttgacaccc
    11701 tttttagtca ttcgtttttg cagtatattg accaagtcgg catcagcatt cggaattaat
    11761 tggtccataa attcaacaac tgttacatta actcccagcg aggagtagac agttgccatt
    11821 tccaagccaa taataccacc acccaataca agtaagtcac ctttgatatc agcgagttct
    11881 aatgcccctg tagagctaaa tattcgttta tcttctggta taaatggtaa tttaatggat
    11941 tctgagccta ctgcaataat ggcattatca aattcaattt ctacagtccc ttctttcgtt
    12001 tcaacaagaa tttgatgggt acctgaaaac ttacctgtcc cagtaattac ttctactttt
    12061 ctttgtttgg caagagcttt tagtcccccg gtcaactttg caacgacaga gtttttccag
    12121 gcaaccaatt ttttattgtc gaatttaggt ttaccaaatg ctacaccttg ctctgacatt
    12181 tcatgggctt catctactac tttagcaata tgcaataatg cttttgaggg aatgcaacca
    12241 acatttaaac aaacgccacc tagtgagtca aaacgctcga ccaaaaccac ctttttatcc
    12301 aagtcggcag ccctgaaagc ggctgtgtat ccgcctggtc cactacctaa taccactaca
    12361 tctgtcttaa ttttattagc cataagaagc ctcatcaaaa aatatgtttt ctggggttat
    12421 tggcatttca ttcagaacaa ggaaatacca atagctctat tcctttatat gtcagagtaa
    12481 aactcttcta atatcaccta aacaatcaca aagaaagcga gtaaaccggg ctgcctcagc
    12541 gccgtcgatt actctatgat cgtaagaaag tgaaataggc aacatcaatc tgggtttaaa
    12601 ctctttatta tcgtatatgg gttttattat tgatcttgat aagcccaaaa ttgcaacttc
    12661 cgggctattt acaataggag taaatgccgt tccgcctata ccaccaaggc tggaaattgt
    12721 aaaacaaccg cctgacatat caataggtgt taatcccttt tctctagctt tcgtactgag
    12781 tctggacatt tcctttgcaa tatcaatcac actcaattta tcaacattct ttattacagg
    12841 aaccaccaga ccgttaggtg tatctactgc tatacctata ttataatatt ttttataaat
    12901 taaattttca ccactggtat ctagtgacgc attaaattgc gggtaagcat ggagagcctt
    12961 acaaactacg ctgcaaacaa atgctaacag ggttaatttg taatcttgat ttttagcgct
    13021 ctctgattct gattttctaa acgcttccaa gtctgtaata tctgcttcgt caaattgagt
    13081 gacatgtgga atggttatcc aggagcgatg tacgtttgtt ccagtaagtt ttttaatttt
    13141 atttaaaggc tttgtttcaa ttgaaccaaa tttactgaaa tcgatggctg gatttgacgg
    13201 taagctgaag ccaccagacg tagttttttc acttaaacgt actttgatat aattttgcag
    13261 atcttctttt gtaatacgag attttctccc acttccctga accaaagata aatcgactcc
    13321 aaactctctt gccaaccttc ttacggctgg acctgcagat attaagatac tttttgattc
    13381 agctatctca aggttattaa ttgatattgc ttcggatttt agttcttcat atggcttctc
    13441 tatttccttt atagattgtt ctgagatatt tttaatctgg cttttttcga tttcaggtgt
    13501 ctcagatttt cctagggttt ttagagttag aataggagta ccttgagata ctttatcccc
    13561 taatttaatt ttcatttcga tgactttacc tgcatagggc gaaggaatat ccatcgttgc
    13621 tttatcaccc tctaatgtaa ttaatgcctg atccttttct acttccatcc ctggtttgac
    13681 taaaatatca attacatcaa cgtcatttgc accaccaata tccgggatag atatttcaat
    13741 atctttggaa ctaggtgtag atgcagttgc ctttgtatca acagattgct cttctgctct
    13801 ggtttgcaaa ttctgttttt ctggttcagt cttttgttct tgagaactgg aaatatttgt
    13861 cgttgtatct gacttggctt tgacaatgag atcaccttcg gatactttgt caccgacttt
    13921 aacgagaatt tgcgttaccg ttccagatat tggagaaggg atgtccatgc ttgccttctc
    13981 agactcaagc gtaatcaatg gtgtatctac ttcgatctga tcaccctctt tcactaaaat
    14041 ctcaatgaca tcaacttgat tagcgccacc gatatcagga attttaattt cactttcttt
    14101 tgtcatattt tcctcagcac tcagttaaac cggaactatc ttctgttctg attaattgtt
    14161 taactaatgg gtcattggat ccaatttgtc ttgattaata ttgtaacgtt tcatggcatc
    14221 gacaattttt gttttatcaa gactaccttc agctacaagt gcatttaatg ccgccaacac
    14281 tataaatttt gcatcaactt caaagaaatg acgtagttgt gtacgggtat cacttctgcc
    14341 gtaaccgtct gtacctaggg taatatagcg attaggaaca aaaggcctga tctgatctgc
    14401 ataaatacgc atatagtcag ttgaggcaat aacaggtcca cgacgtcctt ttaattgaga
    14461 tgtcacataa ctttcctgag gcttattttg ggggtgcatg ttattgtatc gctctacagc
    14521 caaaccttct ttacgtaact cattgaagct agttacactc cagatatccg atgtaattga
    14581 aaaatcctct tccagcatct ctgcggcttt gataacttca cgcaaaatag taccgcatcc
    14641 cattagttgg acatggtttt ttgatttttt cttattttct tttaagagat acattccttt
    14701 gataatacct tcctctactc ctgcaggcat atcgggatga gagtagtttt cattcataat
    14761 agtaatgtaa taaaacacgt tttcctgttt ttcatacata cggtgcaatc cattttggat
    14821 gataactgct aattcatagg cgtatgtggg atcataagaa atacaattag gtattgttga
    14881 tgctaagaca tgactatgtc catcttggtg ttgcaatcct tctcctgcca acgttgttcg
    14941 tccagcggtt ccaccaagta gaaatcctct cgcttgcata tctcctgccg cccatgctaa
    15001 atcaccaatg cgttggaaac caaacataga gtaataaatg taaaatggaa tcattgccag
    15061 cttatttgaa ctgtaagagg ttgcggcagc aatccatgaa caaaacgccc cagcttcatt
    15121 aataccttct tcaagaatct gcccgtcaac tgcttctcgg taatacatca cttgctcatg
    15181 atctacagga gtataaagtt gacctacggg agaataaatt ccaatttgtc tgaataatcc
    15241 ctccataccg aatgttctgc attcatctgg tactatgggg actattctgg aattaatatc
    15301 tttatttttg agtagaactg aaagaattcg cacaaatgcc atagtagtcg agatttctct
    15361 atctcctaat cccttggtaa tactggaaaa ttctcctaaa tcaggtattt ttaactgttc
    15421 tacttcagtt gaacggtggg gcaaataacc gcccaaggct tccctttgct tttttatata
    15481 cttgatttcg ggactgtcgt catcgggttt atagaaagga atatcagcaa ttttgtcatc
    15541 gctgattgga atattaaatc gatctctgaa agcttttagt tgatctatgg tcattttttt
    15601 ctgctgatga gtaatgtttt gtccctcacc agccgctccc atcccgtagc ccttgatggt
    15661 ttttgcaagg ataactgttg gagttccctt gtgttcaact gctctggcat aagctgcata
    15721 tactttttga ggatcatggc caccacgatt taatcgccaa atttcctcat cagacatatt
    15781 ttcaaccatt ttctttaatt ctggatattg attaaagaaa tgttgtctta catacgatcc
    15841 atcgttcgct ttgtaagatt gataatctcc atccagacat tcttccatcc ttttctgtag
    15901 ccagccttga ttatcacggg caaataatgg atcccaacgg cctccccaga tgaccttaat
    15961 aacgttccat ccagctcctc gaaataggcc ttcaagttct tgaatgattt taccgttacc
    16021 acgaacaggc ccatcaagtc tttgtaaatt gcaattaact acaaaaatga gattatccag
    16081 cttttctcgt gctgcgatac tgagtgcccc gactgattca ggctcatcca tctcgccatc
    16141 acccaaaaat gcccatactt tcctaccctc agctttgatc agtccccggt tctcaagata
    16201 ttttagaaag cgagcttgat agatagcttg caaaggacct aagcccatgg atacagtggg
    16261 aaattgccaa aaatcgttca ttagccaagg gtgtgggtag gaagataaac catctacctc
    16321 tacttcttgc cgaaattttt caagttgttt ctctgttaat cggccttcaa gaaatgctct
    16381 ggcataaatg cctggagccg agtggccttg aatataaagc aagtctcctc cattttcgcc
    16441 tttttgtcct ttaaaaaaat agttaaatcc agtttcatat aacgttgatg cagatgcata
    16501 agaagcgatg tggccgccaa gctctggcgc gtatttacct gccctaagta ccattgcaac
    16561 agcgttccat ctaattaatg cattgatgcg ctttccaatt ccctcatcgg gaggcatttg
    16621 tttttcttcg tgaggtttta tagtgtttct gtagggagta ttaattgagc tggttagttt
    16681 aaccccctcg gcgttcgctt tattaagaag ctgctgcaaa aggaatgcag tacgctcagg
    16741 accatcatta aagagtacag cttgtagggc atccagccat tcacgagttt ctattggatc
    16801 cagatctaaa tttgtttcat tggtcatgaa atagttcccc aacaatcaat aattttatga
    16861 acagtatggt acgggttgta atcttttctg aattacacca gaacaacctt gattacatgc
    16921 attaaaatcc gcagagcaat tacaagttac tttgcggcat tgtagtggat cacggtaaga
    16981 ttgcaacccg cgagtaatat atccaccttg gattttgact tcgtgaaggt attccagata
    17041 attttctttg gcgaaatggt ttactttcgt agaacaatct cggcagttgt ctgtgcaatt
    17101 ttgtttacag taattaagct gctgaaaaca agtttgttgg cattggctta aggttttatg
    17161 atgggtaatt ttacttttta gtgtggcaca agctgaaagc tccaatatta taaggcaaaa
    17221 aagtaaacgg aatgcagtgt tcatgattca agtcccacat taatttattg tgaaatagaa
    17281 cattatagcc aagctactaa cgtaaataat gcaataaaaa ccaacaacac tattgtggca
    17341 tgttctacaa gattttcagc agcagccatt ggtacttctt cagtatcatt gatacgaact
    17401 gcttgcaaac cacaccctct cagtatttta tcatttgagt caggataagt tagaacatat
    17461 tgagcgaata gatgaaaacc tcgttgaaaa tttccaacta acaaaaacag caaggcggtg
    17521 attctggctg gtatccattc gagaatgtta gtaatttgat ttgcctgagt acttataaaa
    17581 ttaatttctt tacataaagt gattaatctg tatgttaaag ctgctattgg cccagcgatg
    17641 atgtaccaga atacaacagc aaataattgg ctgttaacct cagcgaaata ttctccaatt
    17701 gcatctgttt cagtttgatt agcctgtttc ttcaaaattg ggtaaaaagc attttgcggc
    17761 cccagacagt agtaaaaaat aaacaggcta agaattaggc caaataaacc aaacagaata
    17821 ctttgtagtg aaaaataaac aagaaatgtg agaaaaacaa tgggcaatat gatagctatc
    17881 aaggtagccc aaggattaga aaaacattta tttttatcga tggttttttt aagaaataga
    17941 cagtagttat taaaccagga aaatcgctga taggacactg aatggattaa aaaccgttca
    18001 ctgagcaagc ataatactat taccagtaat ttcatttgat taatccttac gcattttttc
    18061 agataaagct ataggtgttg gatatttcaa tacaacaaga tacgatgaaa cgataaaagt
    18121 taaaatagta gttgcctgga ttaaattgga ggcgacttct gaaatcaggt ttgtactcaa
    18181 ggcgatgctc gcaaccaata aagaaaactc actcccctgc cccaagcgaa ttcctacttc
    18241 ttttgccact tgttttttct caccagaacg ggcaagcaat atataaaata gcaacggctt
    18301 aataatcagt atcaatgtcg ctaatatcac cgctgggata actacctgag ctccaaatcc
    18361 aaaattaaaa gtagcaccta tcgaaaaaaa gaacattaca aggaaaaaat ctcttaatgg
    18421 ctttaaactt tcagcaatga acaatgaaat tgggcttgat gccaaagcta ctcctgctat
    18481 aaaagcgcca atatcttctg ataagcctat tgtttgtgct aaaacggata tccccaaaca
    18541 ccatcctatg gagagtagaa aaacgtattc ctgagttcta tcaaaacgtg ccagcagttt
    18601 tatcaggatg tatctttcaa ataaaaaaga gaacaagcac agggccggta gcgcgataat
    18661 aaccaggatg aaatcactga ttgacaggtt cccatcctga tgggcactat taagtaatat
    18721 caaaagcact atagctataa cgtcctgcat taggagtacg ctaatcatga cttcaccagt
    18781 atgctggtga tgtaaaatgg ttgttggtaa taatttcaag ccaataatag tacttgaaaa
    18841 catcatggct ccgcccaata tcaatgactc agtcactgtg aggccaaacc atctgccaat
    18901 tccgtaggca agtgcagcaa acagaattga gcttacgatt gcaatccagg ttacttttct
    18961 tagcatatga atcaaattct ggggctgtaa atgcaaacct aataagaaaa gcaagaatac
    19021 tattccgatg tcacctatct gcttgacaac cgtcacatcg gatacaagtt tgaatcccca
    19081 aggacctaag atagcaccaa gtgttatata agcaaccaat aatgactgtt tggtgtacaa
    19141 taccaaagta gaaaaaaaag cagctccagc aaatatcaaa aatatagtgt aaaatacaga
    19201 gccatcatgc attgattgaa tacctcttaa gaaaaggtag ttaaaattga attataaagc
    19261 caattttatc cattaatcta ttaattagct attaattatt gtttattaat aataaattgt
    19321 tgcctttagt tagaactagg gtgatatcta ttgatgagaa tgtcatcgat agagtcataa
    19381 tgtgctgata taattaacac aag
//
LOCUS       DACTJE010000048        19263 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.048, whole genome shotgun sequence.
ACCESSION   DACTJE010000048 DACTJE010000000
VERSION     DACTJE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19263
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.048"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1037
                     /locus_tag="I9760_001311"
     CDS             <1..1037
                     /locus_tag="I9760_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HAT5043087.1"
                     /translation="PNAEQAFSSVEFIMRDINFGWLLRYMHSTGASAFFVVIYLHMFR
                     GLLYGSYQKPRELVWLLGMFLYLLLMAEAFFGYLLPWGQMSYWGAEVITSLFGAIPYI
                     GKGLAVWLRGDYSVANPTLQRFFALHVIGIPILMIILVFLHIVALHKVGSNNPEGIDI
                     KKALDKDGKPLDGIPFHPYYTVKDFVGIIVFSILFFAVVFFAPEMGGYFLEHANFVPA
                     NPMVTPDHIAPVWYLAPFYAILRAIPDKLIGVVCMAAAILLLFFLPWLDKSNVRSVRY
                     KGMYSRISITLFAFSFLLLGYLGTTPVTPVRLFLARIAAVIYFSYFILMPIYSRFEKT
                     RPVPTRIGEK"
     gene            1034..1774
                     /locus_tag="I9760_001312"
     CDS             1034..1774
                     /locus_tag="I9760_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HAT5043088.1"
                     /translation="MMKIKVLLVAALAFLMSNAAFTENLTMDYVPVDINDKESLQRGA
                     KMFMNYCSGCHSLKYMRYNRMAQDLGLTTFDGQIAEDLLKNNLIFTQANIYDPIQIAM
                     SPEDAKQWFGMVPPDLSLSARDRGPVWIYNYLKSFYSDPSRPFGANNLLMPDVAMPNV
                     LEPLIGKVILVKDKNSHANSLLVVKRGEMFEGQFDSALRDLVNFLVYVGEPAKLIRYK
                     LGVFVLLFFCIFLIAAYSLKKAYWKQLK"
     gene            2025..2645
                     /gene="sspA"
                     /locus_tag="I9760_001313"
     CDS             2025..2645
                     /gene="sspA"
                     /locus_tag="I9760_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAT5043089.1"
                     /translation="MAIVAKRTIMSLFSDVDDVYSHQVRIVLAEKGVNVEILAVKQDE
                     PGADLLSINPYGTVPTLIDRELVLYEARIIMEYLDERFPHPPLLPVYPVARAEARKMM
                     HRIEQDWYYLMNAILREESVEQARANLLESLTSLDPVFADKPYFLSDEFSLLDCALAP
                     LLWRLPKLGLEIAPNFKGIISYMQRVFKRDSFQTSLTDAERQIRAA"
     gene            2648..3043
                     /locus_tag="I9760_001314"
     CDS             2648..3043
                     /locus_tag="I9760_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAT5043090.1"
                     /translation="MAMTSNKPYLIRAFYDWIVDNDLTPYILVDASNPNVQVPREHVQ
                     HDRIVLNISPSATRGLLLENDRIVFTARFSGQTEQIFVAPNAVLEIYAKENGRGIAFS
                     VDEDDEPPPKTSSSSESATKNKPSLKLVK"
     gene            3171..4652
                     /locus_tag="I9760_001315"
     CDS             3171..4652
                     /locus_tag="I9760_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAT5043091.1"
                     /translation="MNKLENALYRCDQIKACEQQAATLYQKDENALMALAGAEAFSFL
                     QKLYPHVRHIAVFCGAGNNAGDGYVLARLAHEQGFSVTVYQCKSIEDLPSAARNAARQ
                     ALASGVHCQSADEPLDGDAELIVDALLGIGLQGAVYGVIANAIHQINASGLPVLSIDI
                     PSGLNADTGLVENICVNATATITFIGLKTGMYTLDGPDYCGVIHCAHLNLEACTTKLT
                     RFASLLRDSTLPLPLAARKKNSHKGDYGHVLVIGGGPGMPGAVSLAAKAALKTGAGVV
                     TVATWPDYAKSALPLIPEAMIWGVKTAGELMPLLAKATVCVIGPGLGDSDWAKTLFLT
                     AITTQLPMIIDASALRLLSQHPQMDDNWILTPHPGEAASLLSCTARDVQMDRYHAASA
                     IQKQYGGVVVLKGAGTVIQTAEKNIFVCPQGNPGMATAGMGDILSGIIAAFCAQGFTL
                     SDAAKLGVWVHAVAGDKVAEKSGEVGVLASDLLDEIPSILNCI"
     gene            4778..5260
                     /gene="tsaE"
                     /locus_tag="I9760_001316"
     CDS             4778..5260
                     /gene="tsaE"
                     /locus_tag="I9760_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAT5043092.1"
                     /translation="MAIEDNDNFITLDLVSEQESKRFAEKLAFCISAPLVITLSGDIG
                     AGKTTIIRAMLKSLGVISAIKSPTFSLVESYNCGQFHIHHFDLYRIHQEEELEYLGFR
                     DYFSNQSVCCIEWPEHGGKTLPPVDIQFNLIIKGTGRLIQIAALSVAGKRILACLAGE
                     "
     gene            5257..6687
                     /locus_tag="I9760_001317"
     CDS             5257..6687
                     /locus_tag="I9760_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAT5043093.1"
                     /translation="MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQ
                     GSFTHKLFTLSQPNRVVLDLSDTQLAVNISQLGLINSLIQQVRSGSPSKNTLRLVMDV
                     KQPVKVRSSSWKPKGELNGIRIDLVNDTPLVINNRRQLSSPVKTIKAPVQQTQSKISS
                     QPSEQRITVHKTPVQVPHNPSKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAI
                     TLKLKRLIDRQPGMRAVLTRSGDYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNG
                     ASVFALSQRGATSEAARWLAEKENYSELGGVNLGELDDSNGVVRSVLIDLSQTATINA
                     GLEMGGKVLGQLDNFTNLHNNKVEQARFVVLKSPDIPSILVETGFISNPREERNLTNP
                     AYQERLSQAIFQGLKNYFWDHPPHGTRIEAMISPNFHIVKKGETLPAIAARYRVSVNA
                     LQSINRLQARARLMPGQKLVIPGTWS"
     gene            6678..8408
                     /gene="mutL"
                     /locus_tag="I9760_001318"
     CDS             6678..8408
                     /gene="mutL"
                     /locus_tag="I9760_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAT5043094.1"
                     /translation="MVLRIHQLSPAIANQIAAGEVIERPASVVKELLENSLDAGASVI
                     GVDVNYGGLLQITVSDNGSGIVGDDLPLAIAAHATSKIRTLDDLYSIDSMGFRGEALA
                     SIASVAKVTIISKPEEQDNAMMLRVEGENRTLSPCARNIGTTIDVSDLFYNAPVRKRF
                     LKNEKLEFQAIEMVVKRFALSAPQIALTLKHNKKLIFSLPPALSEQAKAIRMGRILGN
                     TFMREAIFLDVEHSGMRLYGWISNHRLQRSQNDRQWIYVNQRMVKDKLLQHAVKQAYD
                     GLLHAGRFPICLLYFTLPASEVDVNVHPTKHEVRFQQPRLVHDFFTSQLTKALQSTLD
                     HSETMEYAYAASSCNEQQNTVSEPSHICNDRESYEGARALSPSWSDNSLSSSITCKGY
                     KKTSSVYHPETEFQWVILNERFILVLIKQQPYLVDIVTLFQEWIEKQLMQKALPLESR
                     PLLISIRYPLPNQWIHKLDHLKHELTRVGIQIEYPNADDLLIRSIPLNAPYLDVRRFF
                     NVLCELDFFDTEQLIALISRSQMFDPKQLTVEERIEMNQLLLELSLQEEKCNTCKALT
                     IDDCRMLLHE"
     gene            8401..9342
                     /gene="miaA"
                     /locus_tag="I9760_001319"
     CDS             8401..9342
                     /gene="miaA"
                     /locus_tag="I9760_001319"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027225287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAT5043095.1"
                     /translation="MNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMN
                     IGTAKPSIHELQRAPHYLIDIKDPVESYSAAQFCTDALSLCAEIIKRGNIPLLVGGTM
                     MYFNALQKGLATLPEADEAVRKRLEEEALSQGWDFLYQKLSQLDPVTAARIHAHDTQR
                     IQRALEVYYLTGSTLSTYLTGPHERPDYYFVNLALFPEQRSWLHERIAQRFDAMLSEG
                     FIEEVQQLQAKWPIQINLPSMRCVGYRQILQHLAGHYDYETMREKGIAATRQLAKRQL
                     TWLRHWEGALFYDSQNVGFNTDIIAKIREILDNTVSN"
     gene            9365..9559
                     /locus_tag="I9760_001320"
     CDS             9365..9559
                     /locus_tag="I9760_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HAT5043096.1"
                     /translation="MRKTTKEPASAKKNYVVHRHQLPLSCPTDEMELWNAHPKVYLPI
                     EKTGVEVCPYCGSRFVLQND"
     gene            9552..10589
                     /locus_tag="I9760_001321"
     CDS             9552..10589
                     /locus_tag="I9760_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HAT5043097.1"
                     /translation="MIKSVCVIRLSALGDVLMLVPLIRTLQNKLPNAQLTWIISKPAF
                     YLVEGMEGVEFIVIDKPKTLADYWRFRKQMLPRQFDVLLAPQASFRTNWLIPFIKAKR
                     KVGYDSHRANDGHRLFIRESISPGLEHTLEGFLKFAEPLGVTEKIIHWDLPITKADYE
                     WAQNHLPEQERPILVVNPAASKPERSWLAERYIEVLKQAQVKWDVKVVLTGGPGDHDK
                     SLAEEIAKEIPVVNLVGKTKPKQLLAVISKAKAVLCPDTGPSHMAAAVGTPVVALHAV
                     TNPSISGPYTFQHLVVNRYSQAIQTVLGVSPEKSVWGTQVHGYEAMKLITVDDVMEKL
                     TEIFSGLMNNT"
     gene            10705..11535
                     /locus_tag="I9760_001322"
     CDS             10705..11535
                     /locus_tag="I9760_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HAT5043098.1"
                     /translation="MQLSSEQCDFFFTNGYLVINNFFSESVCDLLKQRIETLLENNQA
                     EIPKTIFSTQTNEHAKKQYFLDSGDKIHYFFEPGAFDEAGDWLRPFNQSINKIGHALH
                     ELDPVFRQYSRDDRIKKIAHQLGLKTLGLVQSMYIFKQPGIGAEVLCHQDSTYIFGED
                     SDALGFWFAIEDATLENGCLEVIPSPCTTPLKQRMFRRENEIYFENFDSSPWPEENSV
                     PLPVKKGALIILHGRVPHKSQANFSNQSRHAYTLHLVDISLPYPEKNWLQWPNGIPCL
                     "
     gene            11659..12459
                     /locus_tag="I9760_001323"
     CDS             11659..12459
                     /locus_tag="I9760_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5043099.1"
                     /translation="MDNESLLAILNNWSATNYAKGNYILREISSKFLAKISIDSNDYL
                     LDAGCGDGSFTQMLANLVPDGYVLGLDRSKTMIDYANKHCRSINVRFDIGDIQEPIIY
                     GPFDNILSFWCLHWTDLEKSLSNLYHVLKPGGKICAIFSSGKQSTLFEVLNSLQFQNL
                     YPDVAKNVVQSKMMRENYSVQCRRALDNLPFKSISFVVEHCQVELPDLGLFENFIHGA
                     PLFKDLPGKMYQDIVNGMVQFFDEYCKKKYGGKWVYETTPYFLTITRE"
     gene            complement(12532..13062)
                     /locus_tag="I9760_001324"
     CDS             complement(12532..13062)
                     /locus_tag="I9760_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2692 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043100.1"
                     /translation="MPTTFEDKSSKVPSLKSLAANVIQKTNANLFFRLHSLETPPEIK
                     KGFIDKELEALTHELTEDYQTKVEARNEKIEECSSNLSSNECFVKCSAFTLTTLMAGV
                     HVGIYYILKAAAVDPSTQIAYISSIPATICFSMCVGVCLNRQITKCLSSCFTPSVPDK
                     ITVDLDELGRQSHVSP"
     gene            complement(13313..15856)
                     /locus_tag="I9760_001325"
     CDS             complement(13313..15856)
                     /locus_tag="I9760_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAT5043101.1"
                     /translation="MSKPYFFYVSGMNCTSCSNAIESFLRNSNLIKINTFHADMSTPD
                     PKKIVISLSEERENEHESNWKIIKKLIDDTGFTCENHEFPPIKDEKPTLLPEKKQTAA
                     QQEASTLSTIFSSAKKIITSHWLLGGLGCVTGASLMILTMAIGGLPLAVMIPLAGLSI
                     LLTIALGAQSYYEAWKKLVQSRTLTMDTLFSISTVSVLIVSLASFFVPWLPMMFEAGL
                     LIYGFRHIGIAIEDTIKDKISSTKFQDRTPQKVRLYRHSDFKKTRIEEIQPNDIIQVK
                     PGELIPLDSVCEQDTLIYPTIITGAVIPKQFRSGEKILAGMRLANDAKPLTLRVLKTA
                     KESYLARLDESIAQSVLEKAPIEIKTGQLLSYFIPSIIALSILSGIIVGLIATPALAI
                     QCAISVLVSACPCTLGLIIPLAVKTGIHKGAEHGVQFKNSRVLQLAEQIDVVIFDLNG
                     TLTTGVPVVKEYAVLNDADLSSEHFLSICNVLEGQSKHPFGKAIQSFTQGINKQKFNI
                     ANLDHSNHSGISAEINGIQYTIGSKTLMQEIGISTSSLENQMNLVAGDSLVFLARNKQ
                     LLGYLVITDPLRKDAKQTVDALIKMGKEVHLCTGADEKTAYRYAQALGIKKVHANCVA
                     TTINTEDRSKPVYIKSLQNQGLKVAMIGDAANDANALAASDFGIAVLSQDSDEITQQH
                     ASAIIQSGTILPIANAFAISKQTVNNINQNLLISLGYNLTTVLIAGGLLVALGFALNP
                     AIGVALMVTQACFVLLNVYRFKEQPLQHLQDKVQEKPEPAPSSYEAMKKHSPNFHTAP
                     EINHKPAIQSGKQPTNKNKSPSFWSDYFPELFEEEKDTSTEHLMNNKIG"
     gene            16216..17847
                     /locus_tag="I9760_001326"
     CDS             16216..17847
                     /locus_tag="I9760_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein LphB"
                     /protein_id="HAT5043102.1"
                     /translation="MRALLLPRRKTILVLLIAIYCAYDLMVALYTAWDFTTDDAYISW
                     YYARQLASGKGLLWHATLPPVEGYSNFLWIIIAASVIKLQLPLVSSMKVISCCSLGLS
                     LLFLYRLARLFCSPLLSILPVFLFSHYTGVAWWTVSGLETLFYCALSLALIWQCTLAF
                     GYHTVNDSHWINRFSLDSTNHWIITNVLLLLLSLTRFEGIIWIIPLAVFIGCQYLRDH
                     RELVFKDHKRLFLWVSITFVCFTLPYLVYFIWRLIYFGHWIPNSYRCKALAYGQFLAV
                     DLDYFLFITPLLILSVPYLLSAKKDCRHSLLWLPSVLYSLLLWKANPVVTYGLRLFLS
                     PFALFSILPVLGLNQFIQYFKPFRWDPEYPIAGLMLFITWFFVPGNDLNHLSKSVIEY
                     NERAQNRMTIADLLNRKAKKGDSVLLDDCGIIPFFSREDIRFIDAQCLNNSDLTQKPF
                     KNDLSLYADYLNSKVRPDWVITNYYPMESHGDYLTDLLDAKNFFKNYHLASTLSSGFY
                     LNQNQPQGKKMIDFVYQIFKRAYNKPLELESQEPK"
     gene            complement(17854..19254)
                     /locus_tag="I9760_001327"
     CDS             complement(17854..19254)
                     /locus_tag="I9760_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmX"
                     /protein_id="HAT5043103.1"
                     /translation="MKVLPKLALANLVCFTTLSVFAQPADPSGQQTSTNTSNLVTYLT
                     NLGKYLGYDITQSSKAPNPPYSQLFNSNVVQLVQNYAYNTFLGAIPVDAMSQSLMNFV
                     TDKVQGNSLINNLANTTFKYQNFSAPSSGADGKITANGLIDQSTASAQTPTTPGIFSA
                     PQITTSGTYLNDPVSQAVFNILGTPDYSFCMDNEQKNWLPNCNLMYQNLVMQNVIGTL
                     PPAQTSGSTPAFYSYKYNQPLISQLNSNSLIAPLLMDTSASQSGQDSGLTAKSQAQQA
                     LNFIRYASAQVTPPSLPKLSAYSELWNQATAKPNSAGYNEVQQKQAAATLSSYFNNLR
                     VYAAQTSVGVSNLYYILSKRLPQNMSADQSNANITSQALNEFNMATRRLFDPTASNTP
                     GQPNQQWIKQINDASPATVQKEIAILLAEINYQMYLDRQIQERILLTNSIMLLQNLKA
                     AQPTADFSSQGSPSEQ"
BASE COUNT         5530 a         3387 c         4154 g         6192 t
ORIGIN      
        1 cgccaaacgc ggagcaggca ttttcttcag ttgaattcat tatgcgggac atcaattttg
       61 gctggctact ccgttatatg cattccacag gagcttctgc tttttttgtt gttatctatt
      121 tgcatatgtt tagaggcttg ctttatggtt cttatcagaa accacgggag ttagtttggt
      181 tactgggaat gtttttatac ctcttgttaa tggcagaagc attctttggt tatttattac
      241 cctggggaca aatgtcttac tggggtgctg aagtgattac ttccttattt ggagcgatac
      301 cttatattgg caaaggtctt gctgtatggt tgaggggaga ttactctgtt gcaaatccga
      361 cgttgcagcg attttttgcc ttgcatgtca taggaatacc cattttaatg atcatcctcg
      421 tgtttttgca tattgttgca ttgcataaag taggctcaaa caatccggaa gggattgata
      481 tcaagaaggc cctggataag gatggaaagc ctttggatgg aatacccttc catccttatt
      541 acactgtgaa agattttgta ggtatcatag tcttttcaat tttatttttt gcggttgtct
      601 tttttgcccc tgaaatgggg gggtattttt tagagcatgc taattttgtg cccgctaatc
      661 caatggtgac tccagatcat attgctcctg tttggtatct cgcgcctttt tatgccattt
      721 taagggctat tcctgataag ctaattgggg tggtgtgcat ggctgctgcc atattattgc
      781 ttttcttttt gccctggctc gataaaagca atgtgcgctc tgtaagatat aaaggaatgt
      841 attccagaat aagcattacc ttgtttgcgt ttagctttct acttttagga tatttgggca
      901 caactccggt tacaccagta aggttgtttt tagctcgaat tgccgctgtt atttattttt
      961 cttactttat cttgatgcct atttatagtc gttttgagaa aacccggcca gtacctacac
     1021 ggataggtga aaaatgatga aaataaaggt gttattagtt gctgcattgg catttttaat
     1081 gtcaaacgct gcttttacag aaaatttaac aatggattat gtaccggttg atataaacga
     1141 taaagagagt ttgcaaagag gggcaaagat gtttatgaat tattgctcag gatgccattc
     1201 tctcaaatac atgcgttata accgcatggc tcaggatctt ggattaacta cttttgatgg
     1261 tcagattgct gaagatttat taaaaaataa cttaattttt actcaggcca atatttatga
     1321 tcccattcaa atagctatgt cacctgaaga tgccaagcaa tggtttggta tggtgcctcc
     1381 tgatttgtct ttaagtgcgc gagatagagg gcctgtttgg atatataatt atttgaaaag
     1441 tttttacagc gacccttcca gaccttttgg tgctaataat ttactaatgc ctgatgttgc
     1501 tatgcctaat gttttggaac ccttgattgg aaaagtcatt ttagtcaaag ataaaaattc
     1561 gcatgcaaat agtttattag tggtaaaacg aggtgagatg tttgaaggcc agtttgacag
     1621 cgcattaagg gatttagtca attttttggt ttatgtaggt gagcctgcca agctaattcg
     1681 ttataaatta ggagtttttg ttcttttgtt cttctgtatt ttccttatag cagcttattc
     1741 actgaagaaa gcttattgga aacaattaaa ataggaatag gctgcttact cctcagtttt
     1801 gtaaaaagta ctacattttt gccttttata cagagttcaa tctcaaatgg ttgtcttcag
     1861 aatcagaaaa tatatctggt ttcttgtatc gagttcacgc taaaataaga agatttgctc
     1921 aaagtaaccc agaagattaa aaatgtgata gaatagcaac ctttgtgcac tagaatataa
     1981 aaagccattt taacaggctt aaatgaattg ataggagtaa ggtaatggca atcgttgcaa
     2041 agcgcaccat aatgtcttta ttttctgatg tagatgatgt ttatagtcac caagttcgta
     2101 tcgttctggc agaaaaagga gttaatgttg aaattcttgc agtaaagcaa gatgagcctg
     2161 gagctgacct attgtctatt aatccttatg gaaccgttcc aacattaatt gatagggaac
     2221 ttgtattata tgaagcaaga atcattatgg aatatttgga cgaacgtttc cctcatcctc
     2281 cattattacc agtttatcca gtagcccgtg cggaagctcg caaaatgatg catagaattg
     2341 aacaggattg gtattattta atgaacgcta ttttgcggga ggaatctgta gagcaagcca
     2401 gagctaattt acttgaaagt ttaaccagtt tagatcctgt ctttgcagat aaaccttatt
     2461 ttttaagtga tgagttttcg cttttggatt gtgcattggc tcctttatta tggcggttac
     2521 ctaaattagg tttggaaatt gcgcctaatt ttaaaggaat aatttcctat atgcagcgtg
     2581 tgtttaaacg tgattctttt caaacaagtt tgacggacgc tgagagacaa ataagagcag
     2641 cataattatg gcaatgacat caaacaaacc ctacttgatt cgtgctttct acgattggat
     2701 tgttgataat gatttaacac cctacattct cgtagatgca tcaaatccca atgtgcaggt
     2761 tccaagggag catgtccagc atgatagaat tgtattaaac atttctccct cagcgacccg
     2821 aggactttta ttggaaaatg atcgcattgt gtttacggca cgcttttctg ggcaaacaga
     2881 gcaaattttt gttgctccca atgctgtact agagatttat gcaaaagaaa atggcagagg
     2941 gattgctttt tcagttgacg aagatgatga gccgccacct aaaacttctt cttcctctga
     3001 aagtgctaca aaaaataagc catcactaaa attggtaaag tagacggcat ggtttctttt
     3061 aagcgaatcg tagccaagaa gcactgattt ttacagcatg atcttggcat agcgatataa
     3121 aaatattttg tggtcgttat atcaaattta cgttaatttg gagccaagag gtgaacaagc
     3181 tagaaaatgc tctgtatcgt tgtgatcaaa ttaaagcttg tgagcagcaa gctgcaactt
     3241 tgtatcaaaa agacgaaaat gccttgatgg ctctggctgg cgcagaggca ttttctttct
     3301 tacagaaact ctacccgcat gtaaggcata tagccgtctt ctgcggcgct ggcaataatg
     3361 caggtgatgg ttatgtcttg gccagattag ctcatgaaca aggattctca gttactgtat
     3421 atcaatgtaa atcaatagaa gatttacctt ccgctgccag gaatgccgca cgccaggcat
     3481 tggcatctgg agtacattgt caatccgcag atgaaccttt agatggcgat gccgaattaa
     3541 ttgttgatgc attattgggt attggattac aaggagccgt gtatggggta atagccaacg
     3601 cgattcatca aatcaatgcc agtgggttac cggtattatc aatcgatatt ccatctggtc
     3661 ttaatgcgga tacaggatta gtagagaata tttgcgtcaa tgcgactgcc acaattactt
     3721 ttatcggact taaaacagga atgtatactt tggatggacc agattattgt ggtgtgattc
     3781 attgtgctca tttaaacctg gaggcatgta caactaaatt aacccgattt gcttccctgc
     3841 tgagggattc taccttgcca ttgcctctcg cagctagaaa aaagaactct cataaaggtg
     3901 attatggaca tgttttagtg ataggcggag gcccaggaat gcctggagca gtgagcttgg
     3961 cagccaaggc agcactcaag acgggagcag gagtagttac tgttgcaacc tggccggatt
     4021 atgcgaagag cgctttaccc ttaattcctg aagcgatgat ctggggtgtc aaaacagcag
     4081 gggagttaat gcctttatta gcaaaagcta cggtttgtgt tattgggcct gggcttggtg
     4141 atagtgattg ggctaaaacc ttgtttttaa ctgccattac aacccaatta cccatgatta
     4201 ttgatgcttc agcgctgaga ttactttctc aacatcccca aatggatgac aattggattt
     4261 taacacctca tcctggtgaa gctgccagtt tgctttcatg tactgcaaga gatgttcaaa
     4321 tggatagata ccatgcggca tctgctatac aaaaacaata tggtggtgtt gtggtattga
     4381 aaggagcagg cacggttatt cagaccgcgg aaaagaatat ttttgtctgc cctcaaggca
     4441 atccaggtat ggctaccgca ggcatggggg atattttgag tgggattatt gccgcttttt
     4501 gcgcccaggg ttttacctta tccgatgctg caaagttagg ggtgtgggtc catgctgttg
     4561 ctggagacaa ggttgctgaa aaatcaggag aagttggggt attggcgagc gacttacttg
     4621 atgaaattcc aagcattttg aattgtatct agtgcgatga tttattaaga aggtaacctg
     4681 agtgttactg aggtaacaaa ctctgtttca atacttgtgt ataacggttt ggctgacagg
     4741 ctttttctaa acagatacat tatgtagtgt tctcgatatg gctattgaag ataatgataa
     4801 tttcataact ttggatttgg ttagtgaaca agagagcaaa agatttgcag aaaaattggc
     4861 gttttgtatc tcagcgcctt tggtgattac tttaagtggt gatatagggg ccggaaagac
     4921 aaccattatt cgcgcaatgc taaaatcact cggggtaatt tcagccatca agagtccaac
     4981 tttttcatta gttgaaagtt ataattgtgg tcaatttcat attcatcact ttgatttata
     5041 ccgtattcac caggaagaag aactggagta tttaggtttt agagactatt tttccaatca
     5101 gagcgtttgt tgtattgaat ggcctgaaca tggtggaaaa acattgcctc cagtcgatat
     5161 tcagtttaat ttaataatta aagggactgg gcgcttaata caaatagctg cattaagtgt
     5221 cgcgggtaaa agaattttag cttgcctggc gggtgaatga tgatgagagc gtgggtctta
     5281 agtctgttat tatatctacc aagttctttt gctgtatttt ctgcacaatt aaagaatatt
     5341 acagtttctc agcaaccaac catgacgtct ctttatcttt ctatacaagg ttcatttacc
     5401 cataagctat ttaccttaag tcagcccaat cgtgtggttt tagacttaag tgatacacaa
     5461 ttagccgtca atatcagtca attgggttta atcaattcgc ttattcagca ggtaagaagc
     5521 ggtagtccct ccaaaaacac gctcaggctt gtcatggatg taaaacaacc agtcaaggtg
     5581 cgctcatctt catggaaacc gaaaggagaa ttgaatggga tcagaattga tcttgttaat
     5641 gatactcctt tagtcataaa taatcgcagg cagttgagtt cgcctgttaa aacgattaaa
     5701 gctccagtcc agcaaaccca atcaaaaata tccagccagc catcagaaca acgaataact
     5761 gtgcataaaa cacctgttca ggttcctcat aacccatcaa aaaaaccttt gcgtgatgtt
     5821 gttgtagtgt tggatgcagg gcatgggggc aaagatcctg gtgcaagagg tccgcgtcgt
     5881 agtaacgaga aggatgttgt gctggccatt actttaaaac ttaaacgatt gatagacaga
     5941 caaccaggta tgcgcgcagt cctgacacgc tcgggagatt attacgttgg acttagacaa
     6001 cgcctggata ttgccagaaa atataatggt gatgtattca tttctatcca tgccgatgca
     6061 ttcaataatc ctcactctaa tggagcctcg gtatttgcct tgtcacaaag gggggccacc
     6121 agtgaagcag cacgctggtt agcagaaaag gagaattact ctgaactagg tggagtgaat
     6181 ttaggtgaat tggatgatag taatggggta gtacgttctg ttttgatcga tttatcacaa
     6241 actgcaacaa taaatgctgg tttggaaatg gggggaaaag tattaggtca attggataat
     6301 tttaccaatt tgcataataa caaagtggag caagcccgtt ttgttgttct gaaatctcca
     6361 gatattcctt ctattttggt tgaaaccggt tttatctcaa atccccgtga ggaaagaaat
     6421 ttaaccaacc ctgcctatca ggagcgattg agtcaggcaa tatttcaagg actaaaaaat
     6481 tatttttggg atcatcctcc tcatgggact cgaattgagg ctatgatttc tccaaatttt
     6541 catatcgtta aaaagggtga aactcttcct gctatcgctg ctcgatatag ggtatctgtg
     6601 aacgcattac aatcaattaa ccgccttcag gcaagggcaa gacttatgcc cgggcaaaaa
     6661 ttagtgatac ctggcacatg gtcttaagga tacatcaact ttcgccagca atagccaatc
     6721 agattgcagc aggagaagtt atagaaagac ctgcttcggt agtcaaggag ctgctggaaa
     6781 actctttgga tgctggtgcc agtgtgattg gtgtcgatgt caattatggg ggattgctgc
     6841 agattacggt tagtgataat ggttcaggaa tcgttggaga cgacttgcct ttagctattg
     6901 cagctcatgc aaccagtaaa atcagaacgc ttgatgattt atattccatt gatagtatgg
     6961 gctttagagg ggaggcattg gcaagtattg cctctgttgc caaagtgact atcatttcaa
     7021 agcctgagga gcaggacaat gcaatgatgt tgcgagtaga gggggagaac aggactctct
     7081 ctccttgtgc gcgcaatatt ggcactacca ttgatgtttc tgatttgttc tataatgccc
     7141 cagttcgtaa gcggttttta aaaaatgaaa aactggaatt tcaagctatt gaaatggttg
     7201 tgaaacggtt tgcattaagc gctcctcaga ttgcattaac gcttaagcat aataagaaac
     7261 tgattttttc attaccgcca gccttaagtg agcaagccaa agccattcgt atgggtcgaa
     7321 ttttgggcaa tactttcatg agagaagcta tttttcttga tgtggagcac agtggtatgc
     7381 gtctttatgg ctggataagc aaccatcgct tgcaacgcag ccagaatgat aggcagtgga
     7441 tttatgtgaa tcaacgcatg gtaaaggata aattactcca acatgcagtg aaacaagcgt
     7501 atgatggctt attgcatgca ggcagatttc caatttgttt gttgtatttt actttacctg
     7561 cttctgaagt ggatgtgaat gtacatccta caaagcatga agtgagattc caacagcccc
     7621 gcctggtaca tgattttttt acttcccaat tgacgaaagc gttgcaatcg actcttgacc
     7681 attcagaaac aatggaatac gcatatgctg cttcctcttg taatgagcag caaaatacag
     7741 tttcggaacc ttcccatatc tgcaatgaca gagagtccta tgaaggggca agagctttat
     7801 ccccaagctg gagtgacaat tcattatcat catctataac ctgcaaaggg tataaaaaaa
     7861 ccagctctgt atatcatcca gaaacagagt tccagtgggt tatcttaaat gagcggttta
     7921 ttttggtttt gataaagcaa cagccttacc ttgtagacat cgtaacattg tttcaagaat
     7981 ggatagaaaa gcagctaatg caaaaggcat tgcccttgga gagccggcct ttattgatct
     8041 caattcgtta tccattacca aaccaatgga tacataagct ggatcactta aagcatgagt
     8101 tgactcgtgt tggaatacag attgagtacc ctaatgctga tgacttgtta attcgcagca
     8161 tccctttgaa tgcgccctat ttagatgttc gtcgtttttt taatgttctc tgcgaactgg
     8221 atttttttga tactgagcaa ttgatagcgt taattagccg ttctcaaatg tttgacccaa
     8281 aacaattaac agttgaagag cgtattgaaa tgaatcaatt gttgctggaa ctctctcttc
     8341 aagaagagaa atgtaataca tgtaaagcgt taaccattga tgattgtcgg atgttattgc
     8401 atgaataagc tagtgttttg tttaatggga ccaactgctt caggaaagac aggattagca
     8461 tgcgagttgc taactcattt cccttttgaa attattagtg ttgattccgc catgatttat
     8521 agagatatga atattgggac agcaaaacct tcaatccacg aattacagag agcgcctcat
     8581 tatttaattg atattaaaga tccggttgaa tcctattcgg cagctcagtt ttgtacagac
     8641 gctttgtctt tgtgtgctga aatcatcaaa agagggaata ttcctttgtt agtaggtgga
     8701 actatgatgt attttaatgc cctgcaaaaa gggttagcca ctttgcctga agccgatgaa
     8761 gctgttcgta aacgactgga ggaggaagcc ttatcacagg gttgggattt tctttatcaa
     8821 aagctaagtc aattagatcc ggtaacagca gctcgcattc atgctcatga tacgcaaaga
     8881 attcaaagag cgctggaagt ttattactta actggttcaa cgttatcaac ctatttgacc
     8941 ggaccccatg aacggcctga ttattatttt gttaatcttg ctctttttcc tgagcaaaga
     9001 tcctggttgc atgaacgtat cgcgcaacgg tttgatgcga tgcttagtga gggattcata
     9061 gaagaagttc aacaattgca agcaaaatgg cctattcaaa tcaacttgcc ctccatgcga
     9121 tgtgttggat ataggcaaat actccagcac cttgcaggcc actatgatta tgaaactatg
     9181 cgagaaaaag gtatagcagc aacaaggcag ctggccaaac ggcaattaac ctggttgagg
     9241 cattgggagg gggctttatt ttatgattcg caaaatgtgg gatttaatac agatattata
     9301 gcgaaaataa gggaaatatt agataatact gtctctaatt gacatcatgc cgcaaaggaa
     9361 caacatgagg aaaacaacaa aagaacctgc aagtgctaaa aaaaattatg tcgtacatag
     9421 acatcaatta ccattgagtt gcccaacaga tgagatggag ttatggaatg ctcatcccaa
     9481 ggtttatttg cctatagaga aaaccggggt ggaggtttgc ccttattgtg gctctcgttt
     9541 tgtattgcag aatgattaaa tcggtttgtg ttataaggct ttcagcttta ggggatgttt
     9601 tgatgttggt cccactgata aggaccttgc aaaataaatt gcctaatgct caattgacct
     9661 ggattatttc aaaacctgcc ttttatttgg tggaaggaat ggaaggcgtg gagtttattg
     9721 ttattgataa acctaaaacg cttgctgact attggcggtt taggaaacaa atgttgcctc
     9781 gccagtttga tgtgttgtta gcgcctcaag ccagttttag aactaattgg cttattccat
     9841 ttattaaagc taaacgcaaa gttggctatg attctcacag agcgaatgat ggccatcgtc
     9901 tgtttataag ggaaagcatt tctcctggat tggagcacac acttgagggc tttttaaaat
     9961 ttgctgagcc tttaggtgtc actgaaaaaa taatccactg ggatttaccg attaccaagg
    10021 cagattatga atgggctcaa aatcatttgc cggaacaaga aaggccaatt ttagttgtta
    10081 atcccgcagc cagcaaacct gagcgcagtt ggttggcgga gcgatacatt gaggttctga
    10141 aacaggcgca agttaaatgg gatgtcaaag ttgttttaac cggagggcca ggcgatcatg
    10201 ataaatcatt ggcagaggaa atagcgaaag aaattccagt ggttaattta gttggaaaga
    10261 caaaacctaa gcaattgttg gctgtgatca gtaaagcaaa agcagtcttg tgtcctgata
    10321 caggaccttc tcatatggcg gcagcggtag gcacgcctgt tgtggctttg catgctgtta
    10381 ccaatccctc tatttctggc ccgtatactt ttcaacattt ggtagttaac aggtattcgc
    10441 aagctatcca aactgtatta ggagtgagtc cagagaagag tgtttggggt acgcaggtcc
    10501 atggatatga agccatgaaa ttaattaccg tagatgatgt aatggaaaaa ttaactgaaa
    10561 tattttcagg acttatgaac aatacctaat ctttgtgcgg gaaaggcttt taatgagagc
    10621 atttcgctta tgctcattaa aagctgtttg cccttaccct ggtgatttcc ccaagtactg
    10681 attttgattt ttgaggagca taggatgcaa ctttcttctg agcaatgtga tttttttttc
    10741 acaaatggat atttggtaat aaataatttc ttttcagaat ctgtatgcga tctattaaag
    10801 caacgcattg aaacattgct tgaaaataat caggcagaga tacctaaaac aattttttcc
    10861 acgcaaacca acgaacatgc aaaaaaacaa tatttccttg attctggaga taaaattcat
    10921 tatttttttg agccgggtgc ttttgatgaa gccggtgatt ggctacgtcc ttttaatcaa
    10981 tcaattaata aaattggaca tgctttacat gaattggatc ctgtttttag gcaatattcc
    11041 cgagatgatc gaattaagaa gatagcacat cagcttgggt tgaaaacact tggattggtc
    11101 caatcgatgt acatattcaa acagccggga ataggggctg aagtactttg ccatcaggat
    11161 agtacctata tttttggtga ggatagtgat gctttagggt tttggtttgc aatagaagat
    11221 gctactttgg aaaatggctg tttggaagtg attccaagcc cgtgtaccac acccttaaag
    11281 cagaggatgt ttcgtcgaga aaacgaaatt tattttgaaa atttcgattc aagtccttgg
    11341 ccagaagaaa acagtgtccc tttacctgtt aaaaaaggcg ctttaattat actacatggc
    11401 agagttccac ataaaagcca ggctaatttt tctaatcaat ccagacatgc ctatactttg
    11461 catttggttg atatttcatt accttatcct gagaaaaatt ggttgcaatg gcctaatggg
    11521 attccttgtt tataacatca ctagtttaga gatctcagag aatacaacat caaaagcttc
    11581 caatttttag aaaattgtgt tggagaactc tataatttac aagaggctct tggtaaatca
    11641 ttttggaaga aatcccgaat ggataatgaa agtttgcttg ccatattgaa taattggtct
    11701 gcaaccaatt atgcaaaagg taactatatc ctaagagaaa tctccagcaa gtttcttgca
    11761 aagatatcta tcgatagtaa tgattactta ctcgatgctg gttgtggtga tggcagtttc
    11821 actcaaatgc tagccaattt agtccctgat ggatatgttc ttggcttgga tcgatcaaaa
    11881 acaatgattg attatgcaaa caaacattgt aggagtatca acgttcgttt tgatataggg
    11941 gacattcaag aacctataat ttacgggcca tttgataata ttttatcatt ttggtgtctt
    12001 cactggactg atttggaaaa atcattatcc aatttatatc atgtcttgaa accaggaggt
    12061 aaaatttgtg caattttttc ttctggcaaa caaagcactt tgtttgaagt gttaaacagc
    12121 ctgcaatttc aaaatcttta ccctgatgtc gctaaaaatg tcgtgcaatc taaaatgatg
    12181 cgggagaatt attctgttca atgccgaagg gcattggata atttgccatt taaaagcatt
    12241 tcttttgttg tagaacattg ccaagtggaa ttacctgatc tcgggttatt tgaaaacttt
    12301 atacacggtg ccccattatt caaggatctc cccggtaaaa tgtatcaaga tattgtaaac
    12361 ggcatggtgc agttttttga tgagtactgc aaaaaaaaat acggtggaaa atgggtgtat
    12421 gaaaccactc cctatttttt aacaattacc agggagtgaa gaatcattgt aattaaatta
    12481 tattttagcg aataagtcgg cttttttctt tcaaggaatc cagaaaaccg cttatggact
    12541 aacatgggat tgtctaccca actcatccaa atcgacagta attttatcgg ggacactggg
    12601 cgtaaaacaa gaactcaagc atttggtaat ttgtcgattc aagcagactc caacgcacat
    12661 agagaaacaa atagtagcgg gaatactcga aatatatgct atttgcgttg atggatcgac
    12721 agctgctgct ttcaggatgt aataaatacc aacgtgcact ccagccatca atgtagtgag
    12781 ggtaaaagca gagcatttta caaaacattc attagaacta agattggaac tgcattcctc
    12841 aattttttcg ttcctggctt ccaccttggt ttgatagtcc tcagttaatt cgtgggttaa
    12901 tgcctctagc tccttgtcaa taaacccttt ttttatctca gggggagttt caagcgagtg
    12961 caacctgaaa aagagatttg cattggtttt ttgaatgaca ttagcagcca gggattttaa
    13021 actggggact ttggatgatt tatcttcaaa ggttgttggc atgagttttc ttaaaatgta
    13081 agttaattaa ttttgccaat ttgaccatat aatttattta ttgtctatat aatgatgcgg
    13141 tatatactgc tgattacctc atttcacatg cgatttttat gagtcatcat atattggtaa
    13201 gagacatcac attcttgatc ctttagagca taataaatca aaattgattg ttatgattga
    13261 ggaaggtgat tggccttcct catttcaaat tcagccaagc tttatttagg gcttatccta
    13321 ttttgttatt cattaggtgt tcggtgcttg tgtccttttc ttcctcaaat aattcaggaa
    13381 aataatcact ccaaaaggaa ggtgatttat ttttattggt cggctgcttt ccagattgaa
    13441 tggctggttt atgattaatc tcgggagctg tatggaaatt gggtgaatgc tttttcatgg
    13501 cctcataaga agaaggagcc ggttctggtt tttcctgaac tttgtcttgc aaatgttgca
    13561 agggttgctc tttaaagcgg tatacattga gcaagacaaa acaagcctgg gttaccatta
    13621 atgccacacc aatagcaggg tttagcgcaa aaccaagggc gactagcaga ccgccggcta
    13681 ttaatactgt tgtcaaattg taccctaagc taattaacaa attctggttg atattgttta
    13741 ctgtttgttt tgatatggca aaggcattgg ctataggcaa tatagttcca ctttgtataa
    13801 tggctgaggc gtgttgctgg gtgatttcat cgctgtcttg agacagtact gctataccga
    13861 agtcgctcgc agcaagagcg tttgcgtcat tagccgcatc tccaatcata gctactttta
    13921 gtccctgatt ttgtaatgat ttaatataaa ctggtttgga tctatcttct gtattgatag
    13981 ttgtagcgac acaattagca tgtactttct taatgccaag agcttgcgca tagcgatagg
    14041 cggttttttc atctgcacca gtgcatagat gcacttcttt tcccatttta attaatgcat
    14101 caactgtttg ctttgcatct tttcgtaagg ggtcggtgat gaccagataa cctaatagtt
    14161 gtttgtttct ggctagaaat accaggctat cgcccgcaac cagattcatt tggttttcaa
    14221 gcgaagatgt agaaatccct atttcctgca ttaaggtttt actgccaata gtatattgaa
    14281 taccattaat ctctgctgaa atgcctgaat ggtttgaatg atccaaatta gcaatattga
    14341 atttttgttt attgattcct tgagtaaacg attgtattgc ttttccgaaa ggatgttttg
    14401 attgcccctc caatacattg caaatactca gaaaatgctc agaagaaaga tcggcatcat
    14461 ttaataccgc atattcttta accacaggaa cgccggttgt taaagtgcca ttcaagtcaa
    14521 aaatgactac atcaatttgt tctgctaatt gtaataccct ggaattttta aactgcacac
    14581 cgtgttcggc tcctttatga attccagttt ttactgctag aggaataatt aaaccaagag
    14641 tacaagggca agcagaaacc aatactgaaa tagcgcattg tatcgcaagt gctggtgtag
    14701 caatcaagcc gacaatgatc cctgaaagga tggagagagc aataatggat gggataaaat
    14761 agctaagaag ctgccctgtt tttatctcta tcggtgcctt ttcaagtaca gattgagcta
    14821 tgctttcatc aagacgggca aggtatgact cttttgcagt ttttagaacg cggagggtga
    14881 gtggttttgc atcatttgcc agacgcattc cggcaagtat tttttcacca ctacgaaatt
    14941 gtttaggtat gacagcaccg gtaatgatgg tgggataaat gagggtgtct tgctcacaaa
    15001 cgctatccaa tggtataagc tcacctggtt tcacttgtat aatgtcattg ggttgaattt
    15061 cttcaatcct cgttttttta aaatcagaat ggcgatataa tctgactttt tgtggggttc
    15121 tgtcttgaaa cttggttgag cttattttgt ctttgatagt gtcctcaata gcgataccaa
    15181 tatgcctgaa tccataaata agtaatcctg cctcaaacat cataggtaac cacggtacaa
    15241 agaatgaagc aagcgacaca ataagaacag aaacagtgct aattgaaaac aatgtgtcca
    15301 ttgtgagtgt gcgagattgg actaactttt tccaagcctc gtaataagat tgggcaccaa
    15361 gcgcaatggt taataaaatg ctgagacccg ctaggggaat cattacagca agaggtaaac
    15421 cgcctatagc catcgtcagg atcattaagc tagcaccagt gacacaacct agccctccaa
    15481 gcaaccaatg ggaagtgatt attttttttg cgctgctaaa tattgtggat aatgtgctgg
    15541 cttcttgttg agctgctgtc tgttttttct cggggaggag agtaggtttc tcatctttaa
    15601 ttggagggaa ttcatggttt tcacaagtaa accctgtgtc atcaattagt tttttaatga
    15661 ttttccagtt gctttcatgt tcattttctc tttcttcaga aagagatatc acaatttttt
    15721 tggggtctgg ggtgctcatg tctgcatgaa atgtattgat tttgattaaa ttggaatttc
    15781 ttaaaaagga ctctatggca ttactgcaac tggtgcaatt cattccgctg acataaaaaa
    15841 aataaggctt ggacataatc gctcgctgag ttaaatggtt aactcatcct gagaactttg
    15901 taccggattt atcaatcaac accaaccatc gagcaaaaat tgcattttaa tgccaggtct
    15961 atcattttta gtccagagga tgtgcatcac tgtcattgaa atgatattgt gatattccct
    16021 tataccaata acctggactt ttggtgtttg ttgtaagtcc cgtattattc cattgaggat
    16081 acagcatttt taaattaggt taaaatcata tcatacccat tatttttttt gcatcttgat
    16141 tttagagctt gttgatattt tgtgatattg ctattgtgaa ttgggttata aataattttt
    16201 ggagcagaac acattttgcg tgccctccta ttgccaagaa gaaaaaccat tctcgtgcta
    16261 ttgatagcca tttattgcgc ttacgacctg atggttgcat tgtatacagc ttgggatttc
    16321 acaacggatg atgcttatat cagctggtat tatgcgcgac agttagcgag tgggaaagga
    16381 ctattgtggc atgcgactct gcctccagta gaggggtatt ccaatttttt atggatcatc
    16441 attgctgcat cggtcattaa attgcaatta cctctggtta gcagtatgaa ggttatttcc
    16501 tgttgtagct tgggattgtc attactgttt ttatatcgat tggcaagact tttttgtagc
    16561 cctctattat ccatattacc cgttttttta tttagccatt atacaggagt ggcttggtgg
    16621 acggtcagtg gattggaaac cttattttac tgtgccctat ctttggcatt gatatggcaa
    16681 tgtaccttgg cattcggtta tcacactgtt aatgacagtc actggatcaa tcgattttcc
    16741 cttgactcaa ccaatcattg gataataacc aatgtgctct tgttattatt gagcttaaca
    16801 cgatttgagg gaatcatttg gataattcct ttggctgttt ttataggatg tcaatatttg
    16861 agagatcaca gggagctggt ttttaaagat cataagagat tgttcttatg ggtttcaatt
    16921 acttttgttt gctttacatt accttatctt gtgtatttta tttggagatt aatttatttc
    16981 ggtcattgga ttcctaatag ctatcgttgc aaagcgttag cttatgggca gtttttggca
    17041 gtagatttgg attattttct ttttataaca cctttactta tattatcggt accctattta
    17101 ttatctgcta agaaagattg tcgccattct ttactttggt tgccctctgt tttgtatagt
    17161 ctgttgcttt ggaaagcaaa tcctgtggtt acctatggtt taagattgtt tttaagtcca
    17221 ttcgctcttt tttccatatt acctgtttta ggacttaatc aatttataca atattttaaa
    17281 ccctttagat gggatcctga atatccaatt gcgggattaa tgttgtttat tacctggttt
    17341 tttgtgccgg ggaatgattt aaaccattta tcaaaatcag ttatagaata taatgaacgt
    17401 gctcaaaata gaatgactat tgctgattta ctcaaccgga aagcaaaaaa gggcgacagc
    17461 gttctgttag atgattgtgg gattattccg tttttttctc gagaagacat tcgattcata
    17521 gacgcgcaat gtttgaataa tagcgattta acccaaaaac catttaaaaa cgatttaagt
    17581 ctttacgccg attatctcaa ttctaaagtg aggcctgact gggtaattac caattactat
    17641 cctatggaat cacatggtga ttatttgaca gatttgttag atgcgaaaaa tttttttaaa
    17701 aactatcatt tagctagtac attaagctcg ggcttttatt taaaccaaaa tcaaccgcaa
    17761 gggaaaaaga tgattgattt tgtttatcaa atttttaagc gggcgtacaa taagcccctg
    17821 gaattagaat cccaggagcc aaagtgatga ccattattgc tcagaaggag agccttgact
    17881 tgaaaaatca gcagtgggtt gagcggcttt taaattttgc aatagcataa tgctattggt
    17941 taataatatt cgttcttgta tttgtctgtc caaatacatt tgataattaa tttcagccag
    18001 caatatcgct atttcttttt gaactgtagc aggggatgcg tcattaattt gtttaatcca
    18061 ttgttggttt ggttgtccag gagtgttgga ggcggtagga tcaaataatc gtcgagtagc
    18121 catgttaaat tcattcagcg cttggcttgt gatattagcg ttactttggt ctgcactcat
    18181 attttgaggt aaccttttgg ataaaatata gtagaggttg ctcacgccaa cagaagtttg
    18241 cgctgcataa acacgcaaat tattaaaata actgcttaat gttgcggctg cttgtttttg
    18301 ttggacttca ttatagcctg cagaattggg ttttgcagtg gcttgattcc ataactcact
    18361 gtatgcactc aacttgggta aggaaggagg ggtgacttga gcgcttgcgt atctgataaa
    18421 attaagcgct tgctgggctt ggctttttgc cgttaatcca gaatcttgcc cagattgtga
    18481 cgcagaggta tccatcagta agggggcaat caaagagtta ctgttcagtt gacttataag
    18541 cggttgattg tatttgtagg aataaaaagc aggcgttgaa ccagaggttt gtgctggcgg
    18601 caaagtgcca attacatttt gcatgaccag attttgatac atgaggttac aattgggtaa
    18661 ccagttcttt tgttcattgt ccatacaaaa actgtaatcc ggggtgccca gaatattgaa
    18721 tactgcctga ctgactgggt catttaagta tgtcccggag gttgtgattt gaggcgctga
    18781 aaaaatgcca ggtgttgtgg gtgtttgggc actagctgtt gattgatcta ttaagccgtt
    18841 cgctgtaatt ttcccatctg cgccggagga tggggcgctg aaattttgat atttaaaagt
    18901 agtatttgcc aaattattaa ttaatgaatt tccttgtacc ttatcggtaa caaaattcat
    18961 taagctctgc gacatggcat cgacaggaat agcgcccaaa aaggtattat aggcgtaatt
    19021 ttgaacgagt tgaactacat tggaattgaa taattgcgaa taaggcgggt taggggcttt
    19081 gctggattga gtgatatcat agcctaaata ttttcccagg ttcgtcaggt aggtcaccag
    19141 attggaggtg ttggtgcttg tctgttgtcc agatgggtca gcaggttgag caaaaacaga
    19201 taaagtggta aaacaaacca gattggccaa ggccagttta ggtaatactt tcattattca
    19261 tcc
//
LOCUS       DACTJE010000049        18812 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.049, whole genome shotgun sequence.
ACCESSION   DACTJE010000049 DACTJE010000000
VERSION     DACTJE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18812
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.049"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..507
                     /locus_tag="I9760_001328"
     CDS             <1..507
                     /locus_tag="I9760_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2271 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043104.1"
                     /translation="FNKTENEDDGLEVVEEYENFISLVSQVKSGQINADKAFETIKDT
                     TESRQADVIIANFFKVCELMFWATAAFFSYVACVSVGIPLTFCDPLLGIAVSLSTFLM
                     AISTASKFLDCFDEFQSLDKINEHDQNQKDTVRFFSKTSNSTSSVRNKEAEVEESSQR
                     LYPNLQTV"
     gene            complement(682..1170)
                     /locus_tag="I9760_001329"
     CDS             complement(682..1170)
                     /locus_tag="I9760_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 domain-containing protein"
                     /protein_id="HAT5043105.1"
                     /translation="MIIRFSKVLLVVAVSFFCLLTAFGNITDYSANFPAVVKVLTMSD
                     IFPDSTITYRAITSPVLHYVAFIIIVILELLTAIFCGIGAWKLFKARNESASVFNHSK
                     NWAIGGLTLGFVTWQVIFMSIGGEWFGMWMSPTLNSALTTAFHIFITILAVMIYLVIK
                     DE"
     gene            complement(1315..2628)
                     /locus_tag="I9760_001330"
     CDS             complement(1315..2628)
                     /locus_tag="I9760_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAT5043106.1"
                     /translation="MKILFLLLLIFLSVIPAQAKPVELVFWHAMAGHLGDEVRLLADD
                     FNKSQNQYRVKPIYKGNYTETLTNFAAAFRARQAPSIVQIFEVGTSIMLAPPGVIKPV
                     DLLMSEQGISLPKDDFIQSVREFYSRDGQLMAMPFNLSAPVLYYNADILAKVGYGKHN
                     FPQTWSAMEIMAEKIKKAGYDCTYTTAYPGWVLFESFLAIHGLPITQGNPARAVFHTP
                     QLMAHFQRLKRWHDLHYFRYGGRVDDATILFTSSVCPLFSQSSGAYNSLSALVPFHLG
                     VATMPLDTGASPIRHANVAGGAALWAVGGQTEEQYRGIARFFAFIAKPEVQKRWHEHT
                     GYLPLGLKGIYASIVQSSQHPALLLARTDLEDNLPEKPYKHMGPQNQIRGINDEVLEA
                     MFADLMSPEEALNEATIRANHLLLRFARNTQRERVVDDKVGKGLG"
     gene            complement(2625..3344)
                     /gene="ugpQ"
                     /locus_tag="I9760_001331"
     CDS             complement(2625..3344)
                     /gene="ugpQ"
                     /locus_tag="I9760_001331"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAT5043107.1"
                     /translation="MILEKIIGHRGASGYAPENTMASFNKALSLGCRFIEFDVMCSLD
                     GEPFIIHDDNLKRTTNGKGEVGLMEADYLRGLDAGSWYSKQYKGEKIPHFTDVLKWLS
                     FSDVQANIEIKPYPGTVEQTTVSVLSHIHRHWPQNKELPLVSCFEWDALVLCRSIAPE
                     MPLGLLLHEWDKDWLQKAKQLECYSIHFNRKILTADRVKAVKDQGYVVCAYTVNRKRL
                     AKKLFDWGVDALFSDYPDLLK"
     gene            complement(3349..4044)
                     /locus_tag="I9760_001332"
     CDS             complement(3349..4044)
                     /locus_tag="I9760_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043108.1"
                     /translation="MRKIAWILGLMVLTPFVWADNETPDLVLDKIFFQVSAKKWVTTQ
                     TALLNVSINATLSNADLVKARADIMDSLNKIAKGEWHLVQFDRSQDSSGLEKLYVQAQ
                     ARVDQSALTNIYQNAKSVSKPGAKYEISGVEFKPSLEETQVVRAKIREQLYQQVNDEI
                     DRMNKAYPKQNYSVSNLVFFEGDNIPQQPRAYQAKEMNALAMAAAPAPLTVSNELILT
                     AMVEAASNRKQGD"
     gene            4357..5430
                     /locus_tag="I9760_001333"
     CDS             4357..5430
                     /locus_tag="I9760_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid dehydrogenase"
                     /protein_id="HAT5043109.1"
                     /translation="MMSVDNIKNDNQVLAEEDFLEYALSHGFGDLHFKVDPQTGMKAM
                     IAIHSTKLGPALGGCRFIEYPNTAAALKDAMRLARGMSFKAASVNLPLGGGKSVIIKP
                     KGFFNRAEYMHRFGEFVNELNGRYITALDSGTELSDMDIIAEHTDYVASLSRYNGDPS
                     PSTSKGVLRGIQAAVAFKLGKDSLKGLHIAIQGLGHVGYALAKQLHELGATLTVADIS
                     PAAVNMAVQELGAKAVGTDIIHKVPCDVFAPCALGAIINDLTIPQLQTSIIAGAANNQ
                     LAHTYHGKILHEKGILFAVDYVINAGGLIFAASKYLQTPEDKVSEQIDSIYTSLTEIF
                     TRSAKDNLPTSEIADTLAKEKLA"
     gene            5445..6101
                     /locus_tag="I9760_001334"
     CDS             5445..6101
                     /locus_tag="I9760_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT5043110.1"
                     /translation="MKHLSLTPELYKYLLDISLREHPALAALRKETSTMELANMQVAP
                     EQAQFMQMLIRLTRAKKVLELGTFTGYSALAMSLALPDDGQVITCDINEGWTKHAHPY
                     WREAKQEHKIKLRLGPALDTLHSLLNEGGEHQFDFIFIDADKTNYLNYYELALKLVTP
                     KGLIAIDNIFWDGKVIDPNDTSGQTREIKKLNQVIKNDSRVFVSLLAIADGMFLVQPI
                     "
     gene            6165..7214
                     /gene="hppD"
                     /locus_tag="I9760_001335"
     CDS             6165..7214
                     /gene="hppD"
                     /locus_tag="I9760_001335"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HAT5043111.1"
                     /translation="MMQNNNPCGLDGFAFLEFSGPDRNKLHQQFSEMGFQAVAHHKNQ
                     DITLFKQGEIQFIVNAASHCQAEAHASTHGPGACAMGFKVKDAKAAFQHAIAHGGIAF
                     QDAPHANHGLPAIQAIGGSVIYFVDEEHQPFSHEWNITSPEPVVGNGLTAIDHLTHNV
                     YRGNMDKWASFYASIFNFQEIRFFNIKGKMTGLVSRALGSPCGKIKIPLNESKDDLSQ
                     IEEFLHEYHGEGIQHIALNTNDIYKTVNGLRKQGVKFLDVPDTYYEMINDRLPWHKEP
                     LNQLHAEKILIDGEADPKDGLLLQIFTENIFGPVFFEIIQRKGNQGFGEGNFQALFEA
                     IERDQVRRGTLKELS"
     gene            7270..8268
                     /locus_tag="I9760_001336"
     CDS             7270..8268
                     /locus_tag="I9760_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAT5043112.1"
                     /translation="MKLASLKSTRSRDGELCIVNRLLTAAIRVPHIASTLQYALDHWD
                     VVEPKLQLVYQQLNDNQIEDAFAFAPELCASPLPRAYQWADGSAYVNHVELVRKARGA
                     EMPADFWTNPLMYQGGSDAFLGPRDPILVSSEAYGIDFESEVAVITDDVPMGIDHTNA
                     AHHIKLLMLVNDVSLRNLIPEELAKGFGFFQSKPASSFSPVAITPDELGSYWDGQRVN
                     LPLISHLNEQLFGQPNAGVDMTFSFPELIQHAAKTRFLTAGTIIGSGTVSNLDRSKGS
                     SCIAEKRMLEMIEFGQAKTPFMRFGDRIRIEMLDGQGYSLFGSIDQIVTKYDKDSL"
     gene            8265..8903
                     /gene="maiA"
                     /locus_tag="I9760_001337"
     CDS             8265..8903
                     /gene="maiA"
                     /locus_tag="I9760_001337"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HAT5043113.1"
                     /translation="MILYDYFRSTACYRVRIALNLKKIAYEKIEVHLVNNGGEQHSLQ
                     YHQINPQELVPSLDINGQILSQSMAIIDYLEEIHPEMPLLPKDPFMKATLKSMALIVA
                     CDMHPLNNLRVLNRLKEQFNANEEQVLEWYHHWLKTGFDAFEEKLGALERDKPVCFGS
                     EVGLADVCLIPQVYNAHRFHFDMASYPIINEINEYCLTLPAFHDAAPEAISS"
     gene            complement(8900..9460)
                     /locus_tag="I9760_001338"
     CDS             complement(8900..9460)
                     /locus_tag="I9760_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043114.1"
                     /translation="MLQRVLLVLLCFFSFLASASDTVLKLYRPFGDVFEQAPPVIKKI
                     MSGQCYEQSHIIIREDAWRCQAEGQLFDPCFVKAGSKKMEAVCPQSPWVGDSVQINVE
                     VPLNNEYHKTLDMSRAFPWAIELVNGEYCLAINSNEQYDSMPVRYRCSNQNVLIGYLQ
                     RCKTAWSMLEKTPKGVITVELRRAWF"
     gene            9608..11011
                     /gene="asnS"
                     /locus_tag="I9760_001339"
     CDS             9608..11011
                     /gene="asnS"
                     /locus_tag="I9760_001339"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAT5043115.1"
                     /translation="MTEVFTIKQCLDGEISIDETVTVRGWVKTRRDSKAGLSFISLHD
                     GSCFSPIQIVATDQLSNYHKEVTKLTAGCSMIATGKLVASQGKGQFFEIQAESIEVVG
                     WVENPDTYPIQAKRHTLEFLREVAHLRPRTNTISAVTRVRHSLAQAIHRFYHEQGFFW
                     VHTPIITASDCEGAGEMFRVSTLDLLNIPKNDKGQIDFSKDFFGRETFLTVSGQLNVE
                     AYCMAMSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFANLEDICKLSQNMLRYLCK
                     TVLEERADDMDFFNQFVAPGCIERMEHIADSEFEIMTYTDAIKALEASDQKFEFPVSW
                     GLDLQSEHERYLAEVLCKKPVIVTNYPQEIKGFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEILDRRMDECNLNKEHYQWYRDLRRYGTVPHAGFGLGFERLISYVTGVS
                     NVRDVIPFPRTPGHADY"
     gene            complement(11008..11406)
                     /locus_tag="I9760_001340"
     CDS             complement(11008..11406)
                     /locus_tag="I9760_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043116.1"
                     /translation="MDEKKDKTIQINEMTSTVEDEKKAQPLRKSERSLPVKSHRRSFS
                     IFYPYSTENSKVLSSEQASNKGFLPGPDFNREQYDTVDTGGPLASIPEDEILEKIFSP
                     RNRRRLLSEGNQPQQQTAEVEEDSLSALSI"
     gene            complement(11430..12113)
                     /gene="lolD"
                     /locus_tag="I9760_001341"
     CDS             complement(11430..12113)
                     /gene="lolD"
                     /locus_tag="I9760_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAT5043117.1"
                     /translation="MNDIILTSQKLYKSYHDGTSTVEVLKGVDLAITKGDRIAIIGPS
                     GSGKSTLLHLLGGLDKPTSGLITLGNVNWQKINEKQRCQLRNQQLGFVYQFHHLLPEF
                     TALENVMMPLLLAGMAVKDAEEKAINMLEHVGLKPRLAHKPAQLSGGERQRVAIARAL
                     VHQPHCVLADEPTGNLDEATASKVFDLMLELNKKMNTALVIVTHDQRIAERMDRVLVL
                     HEGSLYARE"
     gene            complement(12106..13353)
                     /locus_tag="I9760_001342"
     CDS             complement(12106..13353)
                     /locus_tag="I9760_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HAT5043118.1"
                     /translation="MYKPLALFIGLRYTRSRKKNHFVSFISLSSMLGIGMGVMVLITV
                     LSVMNGFDQEIHRRFFGMAPEITITGPDERLSDWPEVVKKVETIPGIKAIAPYVGSQG
                     LLTHEGQVLPIVLTGILPEKEQSVTHLNKKLLAGNMSNLKHFGIILGKGLADSLGVMI
                     GDKVTIMIPQATVTPAGMIPRFKRFTVVGVFSAGTGFNFDTKLAFINIEDAQKLMQMD
                     KNDVSGIKMKINNVYKAPELSYELSDLLGEGYQVGNWTQQFGAFFEAVKMEKTMMFMI
                     LLLIIAVAAFNLVSSLVMVVNDKQAEIAILRTIGATPSTILWVFIVQGMMVGLVGTIL
                     GLLGGLVLANNATEIVNALQSFFQVKVLSSSIYFVDYLPSKIMFRDLWQVCAMALLMS
                     FAATIYPAWRASKTVIAEALHYE"
     gene            complement(13592..14470)
                     /locus_tag="I9760_001343"
     CDS             complement(13592..14470)
                     /locus_tag="I9760_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAT5043119.1"
                     /translation="MSLYRELENIYKAGPTHMVGDGFRVINFIPGDSDLAKRLNPFVL
                     LDYNPSFYFPPTDSPLGVGPHPHRGFETVTIAYKGAVAHHDSDGNHGIIEAGDVQWMT
                     AGAGILHKEYHATHFAKQGGLLQMVQLWVNLPALYKNTPPAYQALTKQQMGLYHFSNQ
                     QGLLNIIAGNYDGVHGPAKTFTPINLFDIRLLAQSEAILELPSDHNVAILVLEGKITI
                     NQNQSAHENQLLVFSLSAGKIKINSEGKNAILLVLSAEPLLEPIAHHGPFVMNTREEL
                     QQAFIDFYEGKFGNLE"
     gene            complement(14484..15104)
                     /locus_tag="I9760_001344"
     CDS             complement(14484..15104)
                     /locus_tag="I9760_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAT5043120.1"
                     /translation="MKLLAIDSSILTNTSVSRQLTRSFVSRWQKIYPETEVVYRDLHA
                     QPINHLSQKILAANSVPSTQISAEIREEMNLSMQLISELLSASVLVIGAPMYNFSIPS
                     QLKSWIDRIVIAGKTFKYVDGKVQGLATGKRAYILSSRGGFYNAEPALNLDHQERYLT
                     SILNFIGISDITFIRAEGVNVGEEIRTQSLHQAEAKIQQLLQFQMA"
     gene            15253..16173
                     /locus_tag="I9760_001345"
     CDS             15253..16173
                     /locus_tag="I9760_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT5043121.1"
                     /translation="MLPDLNDLYFFYLVVEQRSFTKAGRSIGITKSKISRRISELESR
                     LGVRLLHRSTRKLALTDIGQIFYQHCKAMVDEAQSAQEAIELVQSKPRGRIRVTCPAL
                     FAQSTFTDIITRFMQQYQEVTIVLNATDRPVDLIQEGFDVAIRFQANTFEDSTLVVRK
                     LGLSFSSLIASPTYLNHHGTPQTPQDLVNHACFAKTRNEGMSQFLLTHNDGRQISVHF
                     QPILESNEWLVLKQAALSHLGITMMPQEICRKEIDSGLLVPILTEWSLPKASLYIIYP
                     SRRGLIPAIRSFIDFVAEELINNCTNIRLK"
     gene            complement(16192..16278)
                     /locus_tag="I9760_001346"
     tRNA            complement(16192..16278)
                     /locus_tag="I9760_001346"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16242..16244),aa:Leu,seq:taa)
     gene            complement(16296..16369)
                     /locus_tag="I9760_001347"
     tRNA            complement(16296..16369)
                     /locus_tag="I9760_001347"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16335..16337),aa:Cys,seq:gca)
     gene            complement(16430..16505)
                     /locus_tag="I9760_001348"
     tRNA            complement(16430..16505)
                     /locus_tag="I9760_001348"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16470..16472),aa:Gly,seq:gcc)
     gene            complement(16558..17121)
                     /gene="pgsA"
                     /locus_tag="I9760_001349"
     CDS             complement(16558..17121)
                     /gene="pgsA"
                     /locus_tag="I9760_001349"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAT5043122.1"
                     /translation="MSTLTSLPNILTLMRISIIPVFIMVFYFPFKWAHPTAAVLFAIA
                     SITDWLDGYLARKLEMMSHFGAFLDPVADKLLVSTSLLLLVGAKDIDYITIPAIVIVG
                     REIVISALREWMAEVGRRASVAVGYIGKVKTSLQMIALVLLLAFNPLVSWWGSLGFVL
                     LYVSAILTIWSMVIYLAIAWPELMKKS"
     gene            complement(17141..17800)
                     /locus_tag="I9760_001350"
     CDS             complement(17141..17800)
                     /locus_tag="I9760_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAT5043123.1"
                     /translation="MSSAYQLVIFDWEGTIADTLGVILHTVATEAKMLGFGDIDPYQA
                     RKYVDLGLVQAVKKTLPNLTAGQQEDLLQAVQSAMISRPTEVRLIPGVLEFIQQLHQA
                     KVDLAIATNKGHNSLIRALQATELDQLFKVTRSAGQVPAKPCPQMLEEIMEEFGRDPS
                     STLMIGDSTTDMEMAKRINVNAIGMDFYHQQEDALKAAGALAVFDDYKLLSAYLMLPE
                     A"
     gene            complement(17793..18740)
                     /locus_tag="I9760_001351"
     CDS             complement(17793..18740)
                     /locus_tag="I9760_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAT5043124.1"
                     /translation="MSEVSYKEIGSEENGQRLDNYLIRILKGVPKSHIYRIIRGGEVR
                     INKKRAQASSRLQIGDSIRIPPIRMTDSKEIFVSDNLAKRLKDSIIFEDSSLLVINKP
                     AGIAVHGGSGLSLGVIEALRKTRHDLSYLELVHRLDRETSGCLLLAKKRSTLRAIQAL
                     LEAREVQKIYWALLTHRWDGKKKITITAPLEKNTLKSGERVVSVQKDGKASETDFKLL
                     ENYHQACWVEASPKTGRTHQIRVHSAYFGHVIVGDEKYGAKVGEVEGIDYQQNRLYLH
                     ARSIQFNLNGVMHVFQADVDDGFGNTLKQLRARSLLSYE"
BASE COUNT         5756 a         3979 c         3511 g         5566 t
ORIGIN      
        1 ttcaacaaaa ccgaaaatga agacgatggc ctcgaagtcg ttgaggagta cgaaaatttc
       61 atttcgttgg tctcacaggt aaaatctggg caaattaatg ctgacaaagc gtttgaaaca
      121 ataaaagata cgacagaatc caggcaagca gacgttatta ttgctaattt ctttaaagtt
      181 tgtgaactca tgttctgggc tactgcagca ttttttagtt atgtagcctg tgtatcagta
      241 ggtatcccct taactttttg tgatcctctc ttgggtatcg cagtctcctt atctaccttt
      301 ttgatggcaa ttagcacagc aagcaaattt ttagactgtt tcgatgagtt ccaatcatta
      361 gataagatta acgagcatga tcaaaatcaa aaggataccg tacggttttt cagtaaaacg
      421 tcaaactcca cttcatcagt acgtaataaa gaagctgaag tagaagaaag ctctcagaga
      481 ttatatccga atttacaaac cgtctaaatt aataaacaat gacacatcag accaaagatt
      541 attttggtct gatgttttgg tttgctttaa agagcataat caaaattaag gcaatacata
      601 agtcacctca tgtttaaata ctttctaaac agccgctccg ataggaggct actctgttac
      661 aaattaatct ctggaatttc tctattcatc cttaatcacc aaataaatca taacggctaa
      721 aatagtaatg aagatatgaa aagctgtggt aagggctgag ttgagcgtgg gagacatcca
      781 cataccaaac cactctcctc ctatggacat aaaaatcact tgccaggtta caaaacccaa
      841 cgtaagacct ccaatagccc agttcttgga atgattaaaa acagaagcac tttcatttct
      901 tgccttaaac aatttccacg caccaattcc acaaaatatg gcggttaata attccaggat
      961 aacaatgata ataaatgcaa cataatgcag aactggacta gtaattgctc gataggtgat
     1021 ggtggaatca ggaaatatat cgctcatggt taatactttt accacagcag gaaaattggc
     1081 ggaataatca gtgatgtttc caaatgcagt aagtaagcaa aaaaaggaaa cagcaacaac
     1141 aagcaacact tttgagaatc tgataatcat catttcatcc tttgaaacaa gttaaaaaat
     1201 atgtatcacc taattgataa gaacgttcgt ggagaggatg cttatgctcg tttctcacca
     1261 agccaggttc gctttcttca agcttcgcta accaaaccga tgaatcaaaa aatcttagcc
     1321 caaacccttg cccactttat catcaactac cctttctctt tgagtatttc tggcaaaacg
     1381 taacaataaa tgattcgctc tgatcgtcgc ctcgttcaga gcttcctccg gactcatcaa
     1441 atctgcaaac attgcctcta aaacctcatc attaattcca cgaatttgat tttgcgggcc
     1501 catgtgttta taaggtttct caggtaaatt gtcctcgaga tctgttctgg ctaataataa
     1561 agcgggatgt tgacttgatt gcacaatgct agcgtaaatt ccttttaacc ccaacggcaa
     1621 ataacccgta tgctcatgcc agcgcttttg cacttcaggt tttgcaataa atgcaaaaaa
     1681 tctggcaatt cctcgatatt gctcttctgt ttgtcctcct acagcccata gagcagcacc
     1741 ccctgccaca ttcgcatgtc ttatcggact ggcccccgta tccaaaggca ttgttgcaac
     1801 acccaaatga aaaggaacaa gggcagacaa actattataa gcccccgatg attgactaaa
     1861 taaaggacaa acactactgg taaataaaat ggtagcatca tcaacacgac caccataacg
     1921 aaaataatgc aaatcatgcc agcgctttag acgttgaaaa tgagccatta attggggagt
     1981 atggaaaaca gcacgagcag gattaccttg agttatgggc aacccatgga tagcaagaaa
     2041 agattcaaat aacacccatc caggataagc cgtagtataa gtacagtcat aaccggcttt
     2101 tttaattttt tctgccatta tttccattgc tgaccaggtt tgagggaagt tatgcttgcc
     2161 atatcccacc ttggctaaaa tgtcagcatt atagtacaga actggtgccg agagattgaa
     2221 aggcatcgcc attaattgcc catccctgct ataaaactca cgcactgatt gaataaaatc
     2281 atctttaggc aaactgatcc cctgctcact catcaacaaa tcaacgggtt tgatcacacc
     2341 agggggagct aacataattg aagtgcctac ttcaaaaatt tgaactattg atggtgcttg
     2401 acgagctctg aatgcagcag caaaattggt aagagtttcc gtataatttc ctttgtaaat
     2461 cggtttgact cgatattgat tctgactttt attaaaatca tcagcaagca aacggacttc
     2521 atcacccaaa tgccctgcca tcgcatgcca aaaaaccagc tcaactggtt tggcctgagc
     2581 aggaatgaca ctcagaaaaa ttaacaaaag caaaaagagt attttcattt taacaagtct
     2641 ggataatcac taaagagagc atcaacaccc caatcaaata actttttggc caaacgttta
     2701 cggtttacag tataggcgca tactacatag ccttgatcct taactgcctt cactcgatca
     2761 gcggttaata tctttctatt gaaatgtatt gaataacatt ccaattgctt cgccttctgt
     2821 aaccaatctt tatcccactc atgcagcaag agccctaaag gcatttctgg agcaatactg
     2881 cggcataata ccaaagcatc ccattcaaaa caggaaacga gaggtaattc tttattttgg
     2941 ggccaatgcc gatggatatg acttagcact gatacagtag tttgctcaac cgttccagga
     3001 taaggtttta tttctatgtt cgcctgaaca tcggaaaaag acaaccactt taatacgtca
     3061 gtaaagtgtg gaattttttc tcccttatac tgcttcgaat accaagagcc agcatcaaga
     3121 ccacggagat aatctgcctc catcagtccc acctctcctt ttccattcgt ggttcgtttc
     3181 aaattgtcat cgtgaatgat aaaaggttca ccatcaagac tgcacatcac atcaaactca
     3241 atgaaacgac aacctaaaga caatgcttta ttaaaagacg ccatcgtatt ttctggagca
     3301 taacccgatg ctccccggtg accgataatt ttttcaagga tcaactaatt aatctccctg
     3361 tttgcgattg gatgctgctt ctaccatagc ggtcaatatt aattcattac tcactgtcaa
     3421 tggtgccgga gcagcagcca tcgctaaagc attcatttct ttcgcttgat aggctcttgg
     3481 ttgctgggga atgttgtcac cctcgaaaaa aaccaggtta cttacagagt aattttgctt
     3541 gggataggct ttattcatac gatcaatttc atcattcact tgctgataaa gctgttcacg
     3601 aatcttggca cgtacgactt gagtctcttc caaactgggt ttgaactcca caccactgat
     3661 ctcgtacttg gctccaggct tactgaccga tttggcattc tgataaatat tagtcagagc
     3721 agattgatcg actcttgcct gagcttgtac ataaagtttc tcaaggccag aactatcctg
     3781 agaacggtca aactgcacca aatgccactc ccctttagca attttgttta agctatccat
     3841 tatgtcagca cgtgctttca ccaaatcagc attacttaga gtggcattga tactgacatt
     3901 gagtaacgct gtttgggtag taacccattt tttagcagaa acttgaaaaa aaatcttatc
     3961 aagcaccaaa tctggtgtct cattatcagc ccaaacaaaa ggagttaaga ccattaaacc
     4021 cagtatccag gcaatttttc tcataatatg ctccattcat aatgtttcta ttggaattag
     4081 tgtataccag caagctagaa gacaccacag cagtttgaac ataatcactc taaaacaaat
     4141 cctatacggc attgcctgca aacagtcgga tttgagtaca ggttgagaaa ataaacagag
     4201 taaaaaagcg gcgtgtacat taaagtacat gagcattttt tagaatgttt ttttctcaag
     4261 atgcgccaaa tacggccgtt tgctaagaat tacattgatt taattgacga atagatctat
     4321 aacttcttcc aaaattattg caagaggagc ttaaaaatga tgtctgttga caatattaaa
     4381 aatgataacc aggttttggc tgaagaagat ttcctcgaat acgcattgtc tcatggattt
     4441 ggtgatttac attttaaggt ggatccgcaa acggggatga aggctatgat tgcaattcat
     4501 agcaccaagt tgggacctgc tttgggtggt tgtcgattta ttgaatatcc taatactgct
     4561 gctgctttga aggatgctat gcgccttgcc agaggcatga gctttaaggc tgcttcagtc
     4621 aatttacctt tgggcggtgg aaaatcagtt attattaagc ctaaggggtt ttttaatcga
     4681 gcagaataca tgcatcggtt tggtgaattc gttaatgaat tgaatggcag gtacatcact
     4741 gctttggata gcggtactga attaagtgac atggatatta ttgctgagca tactgactat
     4801 gttgccagtt tatccagata caatggagat ccttcacctt ctacttccaa gggtgtgtta
     4861 agagggattc aggctgctgt tgcctttaaa ttaggtaaag actctctcaa aggtttgcat
     4921 attgctattc agggtcttgg ccacgtcggt tatgctctgg caaaacaatt acacgaattg
     4981 ggcgcgacat taactgttgc tgatatttca cctgctgctg tgaacatggc ggttcaagag
     5041 ttgggagcta aagcggtcgg cactgacatt atccataaag taccgtgtga tgtatttgct
     5101 ccttgtgctt taggtgcgat tatcaatgat cttactattc cccagttaca aaccagtatt
     5161 attgctggcg ctgccaataa tcagctcgca cacacatacc atggcaagat tttacacgaa
     5221 aaaggcattc tgtttgctgt ggattatgtg attaatgcag gtggtttgat atttgctgcc
     5281 tctaaatact tacaaacgcc tgaagacaag gtaagtgagc aaattgatag tatctatacc
     5341 tctctgactg aaatttttac tcgctctgct aaagataatt tacctactag cgagattgct
     5401 gatactttgg ctaaggaaaa attggcttaa tttggatacc gtttatgaaa catttatccc
     5461 ttactcctga gttgtacaaa tacctgcttg atatttcctt gcgtgaacat cctgctcttg
     5521 cggcattacg caaagaaaca tccactatgg aactcgccaa tatgcaggta gctccagagc
     5581 aagcccaatt tatgcagatg ctgatacgtt tgactcgtgc aaagaaggta ttggaacttg
     5641 ggacattcac aggttatagt gccttggcca tgtcccttgc tttgcctgat gatgggcaag
     5701 ttatcacctg tgatattaat gagggatgga caaaacatgc tcatccttat tggcgtgaag
     5761 ccaaacagga acataaaatt aaattacgcc ttggacctgc tcttgacact ttacactcat
     5821 tacttaatga aggtggagag catcaatttg atttcatttt tattgatgct gacaaaacta
     5881 attatctgaa ttactatgaa ctggctttaa aattagttac acctaaagga ttaattgcca
     5941 tagataatat tttttgggat ggaaaagtaa ttgatccgaa tgatacgagt ggacaaacca
     6001 gagaaattaa aaaattaaat caagtgatta aaaacgattc aagggtcttt gtcagtttgt
     6061 tagctatagc tgatgggatg tttctggtgc aacccattta atctttaaaa tccccggcaa
     6121 gcaatacact gcattgctat gtaaataagt acaaggagaa tctgataatg caaaataaca
     6181 acccctgcgg attagatggc tttgcctttt tagagttttc aggccctgat aggaataaat
     6241 tacatcagca attttctgag atggggtttc aggctgttgc ccaccataaa aatcaagaca
     6301 ttactctttt caaacaaggg gaaatacaat ttatagtgaa tgcggcctcc cattgtcagg
     6361 cagaagcgca tgcttcaact catggtccag gcgcttgtgc aatgggcttt aaagtaaaag
     6421 atgccaaagc cgcttttcaa cacgctatcg cgcatggcgg tatagcattt caggatgcac
     6481 ctcatgccaa tcacggcttg ccagccatcc aggctattgg tggtagtgtt atttattttg
     6541 tcgatgaaga acaccaaccc ttctctcatg aatggaatat tacctcacca gaacccgtag
     6601 ttggaaatgg tctgacagca atcgaccatc tcacccataa cgtttatcgc ggtaatatgg
     6661 ataaatgggc cagtttctat gcttccattt ttaacttcca ggaaattcgt tttttcaata
     6721 tcaaaggaaa aatgacaggt ttggttagtc gagcattagg tagcccttgt ggcaaaatca
     6781 aaattccttt aaacgaatcc aaagatgatt tatcacaaat tgaagagttt cttcatgaat
     6841 atcatggcga gggcattcaa cacatcgctc tcaataccaa tgatatttat aaaacagtca
     6901 acggcttaag aaaacaaggg gtcaaattcc tggatgtgcc agatacttac tatgagatga
     6961 ttaatgaccg cctcccatgg cacaaggagc cactgaatca actccatgct gagaaaattt
     7021 taattgatgg agaagcagat cccaaagacg gcttgttact gcaaatattt actgaaaaca
     7081 tatttggacc agtctttttt gaaattattc aacgcaaagg caatcagggg tttggtgaag
     7141 ggaatttcca ggctctattc gaagctattg aaagagatca agttcgacgt ggtactttaa
     7201 aagaattaag ctagacaaac agttgaaatg taatgatgct gtttttcagg cataagccag
     7261 gagatctata tgaaactcgc cagtttgaaa tcaactcgtt cccgggatgg agagctttgc
     7321 attgtcaaca gattgctgac cgctgcaata cgcgttcctc atatcgcatc aacacttcaa
     7381 tatgccctgg atcattggga tgttgttgaa cccaaattac aactggttta tcaacagtta
     7441 aatgataatc aaatcgaaga cgcctttgcg tttgctcccg agctatgtgc ctcgccatta
     7501 ccccgtgctt atcaatgggc cgatggcagt gcctatgtaa accatgtcga attggttcgt
     7561 aaagccagag gagcagaaat gcctgctgat ttttggacca atccactcat gtatcaaggc
     7621 ggatctgatg cttttctggg gcctcgtgat cctattttgg taagctcaga agcctatgga
     7681 attgactttg aatccgaggt ggctgttatc actgatgatg ttcccatggg gattgatcat
     7741 acaaacgctg cccatcatat caaactgttg atgttggtta atgatgtctc tctacgcaat
     7801 cttatacccg aggaactggc aaaagggttt gggttcttcc aatcaaaacc tgccagcagc
     7861 ttctccccgg tagcgataac cccggatgag ctaggttctt actgggatgg acagcgtgtg
     7921 aatttgccat taattagcca tttgaatgaa cagttatttg gtcaacccaa tgccggagtt
     7981 gatatgacct tttcttttcc agaactgata cagcatgcgg caaaaacaag gtttttaact
     8041 gcaggcacta ttataggttc tggaacagta tccaacctcg accgcagcaa aggctcttct
     8101 tgtatcgctg aaaagcgaat gctggaaatg atcgaattcg gacaggcaaa aacaccgttt
     8161 atgcgatttg gtgaccgaat tcgcatagaa atgctcgatg gtcaagggta ttccctattt
     8221 ggctccattg accaaatagt gacgaaatac gataaggact ccttatgatt ttgtatgatt
     8281 atttccgctc taccgcatgc tatcgagtaa gaatcgctct taatttaaaa aaaattgctt
     8341 acgaaaaaat tgaagtgcat ctggttaata atggtggcga gcagcatagc cttcagtatc
     8401 atcaaattaa ccctcaagaa cttgttccta gccttgacat caatggacaa atcctgagcc
     8461 aatcaatggc aatcattgat tatttggaag aaatccatcc ggaaatgcct ttgcttccta
     8521 aagatccttt tatgaaagca accctaaaat caatggcttt aattgttgct tgtgatatgc
     8581 accctcttaa taatttaaga gtactaaatc gcttaaaaga gcaatttaat gccaatgaag
     8641 aacaggtact ggaatggtat caccattggc taaaaacagg atttgatgct tttgaggaaa
     8701 aacttggagc tttggagcgc gacaagccag tctgctttgg aagtgaggtt ggtctggccg
     8761 atgtctgttt gatcccccaa gtttataatg ctcaccgatt tcattttgat atggcgagtt
     8821 acccaataat caatgaaatt aatgagtatt gtttaaccct accggcattc catgatgcag
     8881 caccagaagc catttcatct taaaaccatg ctcttctcaa ctctaccgtg attacgcctt
     8941 taggggtttt ctccaacata ctccaggctg ttttacatcg ttgcaggtaa ccaattaaaa
     9001 cattttgatt gctgcaacgg taccttacag gcattgaatc atattgttcg ttagaattta
     9061 tagccaaaca atactcacca ttaaccaatt caatagccca gggaaaagcg cgagacatat
     9121 ctaaggtctt atgatattcg ttattcaaag gtacctctac gttaatttgt acactatcac
     9181 caacccatgg cgattgagga caaacagcct ccattttttt actacctgct tttacaaaac
     9241 agggatcgaa taattgacct tccgcctgac aacgccacgc atcttcgcgt ataatgatgt
     9301 gggattgttc ataacattgg ccagacatta tttttttaat gacaggtggt gcttgttcaa
     9361 aaacatcacc aaaaggtctg tataatttta gaacagtatc gctggcgctg gctaaaaaag
     9421 aaaaaaaaca tagcaatacg agtaaaactc tttgcagcat ggtgttcccc cacggatttt
     9481 atttagtgta tcatcacatt ttaattcagg gtttaggaaa tactacaaac gggagaaaat
     9541 caatccctcg aaggattgga atatttccta agccctgaat caatcgtttt cgattttgga
     9601 gtatataatg acagaagtct ttactataaa acaatgtctc gacggcgaaa tcagtattga
     9661 tgaaaccgtt acggtaagag gatgggtgaa aactcgaaga gattcaaaag ctggcttgtc
     9721 tttcatcagc cttcatgatg gctcttgctt ttctcccata caaatcgtcg ctaccgatca
     9781 gttatccaac taccacaaag aagtaacaaa attaactgct ggctgttcaa tgatagcgac
     9841 tggaaaattg gtagcatcac aaggaaaagg gcaatttttt gaaatccagg ccgaatctat
     9901 tgaagtggtg ggatgggttg aaaacccaga cacataccct attcaagcca aacgccatac
     9961 tcttgagttt ttaagagaag tggctcatct gcgccccaga accaatacca taagcgctgt
    10021 caccagagta cgtcattctt tggcgcaagc cattcatcgt ttttatcacg aacaaggctt
    10081 tttttgggtg catacaccga tcattactgc cagcgactgt gaaggagcag gagaaatgtt
    10141 cagagtatct actctggatt tattaaatat ccctaagaat gataaaggac aaatcgattt
    10201 cagtaaggat ttttttggca gagaaacgtt cttaactgtt tctggccaac taaatgtcga
    10261 agcttattgt atggccatgt ctaaggtcta cacttttgga ccaacatttc gggctgaaaa
    10321 ttcgaataca agccgacatt tggctgaatt ctggatgatc gaaccagaaa tcgcttttgc
    10381 aaatctggaa gacatttgta aactcagcca aaatatgctg cgttatctct gtaaaacggt
    10441 tcttgaagag cgtgccgatg atatggactt tttcaatcaa tttgtcgccc caggttgtat
    10501 agaacgtatg gaacatattg cggattctga atttgaaatt atgacctata ctgatgcgat
    10561 aaaagcactt gaggcgagtg accaaaaatt tgaatttcca gttagctggg ggctggatct
    10621 gcaatcagag catgaacgtt acctggctga agtgctttgc aaaaagcctg tcattgtgac
    10681 aaattacccg caagaaatta aggggtttta catgcgtctt aatgacgatg ggaaaacggt
    10741 agccgccatg gacgttttag ctccaggcat cggagagatt atcggcggca gccaacgcga
    10801 agagcgcttg gaaatactgg atagacgcat ggacgaatgc aacctgaata aagaacatta
    10861 ccaatggtat agagacttgc gtcgttatgg cactgttcct catgccgggt ttggcttggg
    10921 ctttgaacgc cttatcagct atgttacagg agtatccaat gtgcgagatg tgataccttt
    10981 ccctcgcaca ccaggccatg ccgattatta aatcgataag gcgcttaaag aatcttcctc
    11041 aacttcagct gtttgttgtt gaggttgatt cccctcagac agcaatcgac gtctatttct
    11101 tggcgaaaaa attttttcta atatttcatc ctctgggata cttgcaagcg gccctcctgt
    11161 atccactgta tcatactgtt ccctattgaa atcaggtcca ggtagaaatc ctttgttact
    11221 tgcctgttct gaagaaagca cttttgaatt ttcggttgag taaggataaa aaatagaaaa
    11281 acttcttcta tgtgacttta caggtagact acgctcggat tttctcaatg gttgggcttt
    11341 tttttcgtcc tcaacagtgc ttgtcatttc attgatctga attgttttat cttttttttc
    11401 atccattgtt aacactcctt atcttatttt tattctctcg catacaaact gccttcatgc
    11461 aaaacaagta ctctatccat acgttctgca atccgttgat catgagttac tataaccagg
    11521 gctgtattca tttttttatt aagttccaac atcaaatcaa aaactttact ggccgttgcc
    11581 tcatccaaat ttccagtcgg ctcatcagcc agaacacaat gtggctgatg caccaaggct
    11641 ctcgctatgg caaccctttg cctttctcct ccagacaact gagcaggctt atgcgctaat
    11701 cgtggtttaa gccctacatg ttccagcata ttaatggctt tttcttccgc atctttcaca
    11761 gccatacccg ccagtaataa cggcatcatc acattttcaa gtgccgtaaa ctcgggtaac
    11821 aaatggtgga attgataaac aaatcctaat tgttgattgc gcaattgaca acgttgtttt
    11881 tcatttattt tttgccaatt gacattgcca agagtaatca ggccgctggt tggtttatcc
    11941 agccccccca acaaatgcaa aagggtactt tttccagaac ctgatggccc gataatagcg
    12001 atcctgtccc cttttgttat ggccaagtcc actcctttta acacctcaac tgttgaagtg
    12061 ccatcatggt aggacttata cagtttttga ctggttaaaa tgatgtcatt cataatgcaa
    12121 agcctccgcg atgactgtct ttgatgctcg ccaggcagga taaatagttg cagcaaaact
    12181 cattaataat gccatagcac aaacttgcca cagatcacga aacataattt tagaaggcaa
    12241 gtaatcaaca aaatagatgc ttgaagacaa caccttcacc tggaaaaagg actgcaatgc
    12301 attaactatc tccgttgcat tattggccaa cactaaacct cctaacagcc cgagaattgt
    12361 accaaccaga ccaaccatca ttccctgtac aataaaaacc cataaaatgg tagaaggagt
    12421 agcgccaata gttcttaaaa tggcaatttc agcttgtttg tcattgacta ccattaccag
    12481 agaggacacc aaattaaaag cagccacagc aataataagc aataaaatca taaacatcat
    12541 cgttttttcc attttaactg cttcaaaaaa agctccaaat tgttgggtcc aattgcctac
    12601 ttgatatcct tctcccaaaa gatcggataa ttcataagac aattcaggtg ctttgtatac
    12661 attattaatt ttcattttta tacctgaaac gtcattctta tccatttgca ttaatttttg
    12721 agcatcttcg atattaataa atgccaattt agtatcgaaa ttaaatcctg taccagcaga
    12781 aaaaacacct acaacggtaa agcgcttgaa tcgagggatc ataccggcag gagttactgt
    12841 tgcttgcggg atcattatcg taaccttatc cccaatcatc actccaaggc tgtccgcaag
    12901 accttttccg agaataatcc caaaatgttt cagattactc atattacctg ccagtagttt
    12961 cttattgaga tgagttactg attgttcttt ctctggcaaa ataccggtta gaacgatagg
    13021 caaaacctgg ccttcatgag ttaataatcc ttgacttcca acataaggcg caattgcctt
    13081 tattccagga attgtttcaa cctttttaac cacttcaggc caatcactta atcgctcatc
    13141 aggacctgtt atggtgattt caggcgccat cccaaaaaaa cgacgatgaa tttcctgatc
    13201 aaaaccattc atcactgata aaacagtgat taaaaccatc actcccatgc ctatacccaa
    13261 catggaactt aaggaaataa aggaaacaaa gtgatttttc ttacgtgaac gcgtataacg
    13321 taatccgata aacagagcca atggtttata cataatcgta ttcagctgga ttaaagttag
    13381 tattgtacag tacttataga aaaggccaag attgaagcct aaagatttta aagaacttat
    13441 taataattca tcatcagaca acccacatag ttatccacag aatctgtgga taactaatag
    13501 ctaggatttc ataatcatgt gttttgggga aatccttttt aaatagtcat ctagtagact
    13561 ttgttttctc agcaaacgta tctcaatttt tttattcaag attaccaaac tttccttcat
    13621 aaaaatcgat aaaagcttgc tgaagctctt cacgagtatt cataacaaaa ggtccatgat
    13681 gagctatagg ttctaacaaa ggttctgcac tcagaaccaa aagaatagca tttttccctt
    13741 cactatttat tttgattttt cctgcactca gtgaaaaaac gagtaattga ttctcatgtg
    13801 cgctctggtt ttggttaata gtaattttgc cttcaagcac caagatagct acattatgat
    13861 cacttggtag ttctaaaata gcctcactct gagcaagtaa acgtatatca aataaattaa
    13921 ttggtgtaaa agttttggca gggccatgaa ctccgtcata atttccagca ataatattta
    13981 ataaaccttg ttgatttgaa aaatgataca gccccatttg ttgcttggtt aatgcttgat
    14041 aagctggagg agtgttttta tagagcgctg gtaaattgac ccataattgt accatttgta
    14101 ataaaccacc ttgtttcgca aaatgtgttg catgatattc tttatgtagg attccagccc
    14161 ctgctgtcat ccattgaaca tctccagctt ctatgattcc gtgatttcca tcactatcat
    14221 gatgagctac cgcaccttta taggcaatag taaccgtttc aaaaccacga tgtggatgtg
    14281 ggcctacacc aagaggacta tctgttgggg gaaaataaaa tgacggatta taatccaaca
    14341 aaacaaaagg atttaatcgt ttagccaaat cactatcacc gggaataaaa ttaatgactc
    14401 gaaaaccatc ccctaccata tgagtagggc ctgccttata aatattttcg agttctcgat
    14461 ataaactcat cgccgactcc tgattatgcc atttgaaact gtaataactg ttgtatcttt
    14521 gcttctgctt gatgtaagga ttgagtgcga atctcttctc caacattcac tccttctgct
    14581 cggataaagg taatatcaga tatgccaata aaatttaaaa ttgaagttaa ataacgctcc
    14641 tgatgatcaa gatttagcgc aggttccgca ttataaaaac caccacggct tgataatata
    14701 taagcccttt ttcctgttgc taatccttgt accttaccat ccacatattt aaaagttttt
    14761 cccgcaataa caatgcgatc aatccaggat tttaattgag agggtattga aaaattatac
    14821 ataggggctc cgataactag tacagaagca cttaataatt ctgagattaa ttgcattgat
    14881 aaattcattt cctccctaat ttcagcagat atttgtgtag atggtaccga attggcagct
    14941 aaaatttttt gcgataaatg gttaataggt tgggcgtgta aatcacgata aactacttcc
    15001 gtttcaggat aaatcttttg ccatcgacta acaaaacttc gggttagctg ccgtgaaaca
    15061 gaggtattag ttaatatact tgagtcaata gctaataatt tcattttatc actccatatt
    15121 tattgattct attattagca tattataaag aacaataaat aataagatgg cttttatatg
    15181 actcattgtc ctataaataa aaccaataaa tgctataatt agcagataac taaacttaca
    15241 caagtgtaaa aaatgttacc tgatctcaat gatttgtatt ttttctatct ggttgtggag
    15301 caaagaagct ttactaaagc agggcggagt ataggtatta caaaatcaaa aatcagtcga
    15361 cggattagtg aattagagtc tcgtttaggg gtgaggttat tgcatcgctc cacaagaaaa
    15421 ctcgcgttga ctgatattgg acaaattttt tatcaacatt gcaaagctat ggttgacgag
    15481 gctcaatctg cgcaagaagc aattgagtta gtgcagagca aaccacgagg gcgtattcgt
    15541 gtcacctgtc ctgcattgtt tgcacaatcg acttttaccg atattattac ccgatttatg
    15601 caacaatatc aggaagtgac tattgtttta aatgccacag atcgccctgt ggatctgatc
    15661 caggaaggat ttgatgtcgc aattcgtttt caagctaata cctttgaaga ttcaacttta
    15721 gtcgtacgca aactggggct tagttttagc tcgttgattg ctagtcccac ttatctaaat
    15781 catcatggaa cccctcaaac accacaagat ttggtcaatc atgcttgttt tgctaaaaca
    15841 cgcaatgaag gaatgagcca atttttatta actcataacg atggtcgtca aatttctgtg
    15901 cattttcagc caattttaga aagtaacgaa tggctggttc taaaacaagc ggcattatca
    15961 catcttggaa taacaatgat gcctcaagaa atttgtcgca aagaaattga ttctgggtta
    16021 ttagttccga tattgacaga atggtctttg ccaaaggcaa gcctttatat aatctatccc
    16081 tctcgtcggg gtttgatacc agccatacga tcttttattg attttgtagc cgaagaatta
    16141 attaataatt gtaccaacat taggcttaag taatttaaac acatcaaatt ttggtgccca
    16201 gggccggaat cgaaccggca tggtgttacc accgagggat tttaagtccc ttgcgtctac
    16261 ctgtttcgcc acctgggcat tcactgtttt tttaatggag gctgaggccg gaatcgaacc
    16321 ggcgtacacg gctttgcagg ccgctgcatg accactctgc cacacagcct caaatctata
    16381 tacttcgcta ttgctctaaa aaaaaagcga cttcgatgtc gcttaaattt ggagcgggaa
    16441 acgagactcg aactcgcgac cctaaccttg gcaaggttat gctctaccaa ctgagctatt
    16501 cccgcgtctc tacgggatga gattctaccg aaaataaata ccctgtcaac agcaaaatta
    16561 actttttttc attaactctg gccaagcaat agccaaataa atcaccatgg accaaatcgt
    16621 caaaatagca gacacataaa gaagaacaaa ccctaagctc ccccaccaag aaacaagggg
    16681 attaaatgcc agcaataata ccaaagcgat catttgcaaa gaagttttaa ctttccctat
    16741 atacccaacg gcaacactag cacgacgtcc aacttcagcc atccactcgc gcaaagctga
    16801 aatcacgatt tctcgtccaa cgattacgat tgcaggtata gtgatataat ctatatcttt
    16861 cgctccaact aacaataaca agcttgtcga taccaataat ttatcggcga cgggatcaag
    16921 aaaagctcca aaatgagaca tcatttcaag tttgcgcgcc aaataaccgt ctaaccaatc
    16981 agtaatgctt gcaatagcaa aaagaaccgc agcggtagga tgagcccatt tgaaaggaaa
    17041 ataaaatacc ataataaata ctggaatgat cgaaatccgc attaaggtta atatattagg
    17101 caaactggtt aacgtactca ctgctgaaca tccccctttt ttatgcttcc ggcaacatca
    17161 gataagctga caatagctta taatcatcaa atacagccaa agcacctgct gccttaagcg
    17221 catcctcttg ctgatggtaa aaatccatgc ctatagcatt aacattgatt ctttttgcca
    17281 tttccatatc cgttgtagaa tcacctatca tcaaagttga tgaaggatct ctcccgaatt
    17341 cctccatgat ttcctcaagc atttgcggac aaggttttgc cggtacttgt ccagcagagc
    17401 gtgtcacttt aaacaattga tcaagctcag ttgcctgcag agcacggatt agagaattgt
    17461 ggcctttatt tgtcgcgatg gccaaatcga cctttgcttg atgaagttgt tggataaatt
    17521 ctaatactcc aggtattaaa cgcacttctg ttggccgtga aatcattgcg gattgcaccg
    17581 cctgtaacag atcttcctgc tgcccggcag ttagattggg caatgttttt tttactgcct
    17641 gaaccaatcc taagtctaca tattttcttg cttgatatgg gtcaatatca ccaaagccca
    17701 acattttagc ttcagtagca acagtatgta aaataactcc caatgtatca gcaatcgtgc
    17761 cttcccaatc aaaaattact aattgatatg cactactcat agctcaatag gctccttgca
    17821 cgcagttgct ttaaagtatt tccaaatcca tcgtctacat cagcttgaaa tacatgcatt
    17881 accccattca gattgaattg aatagacctt gcatgcagat aaagacgatt ctgttgataa
    17941 tctatacctt ccacctcacc caccttagcg ccgtatttct catcaccaac aatgacatga
    18001 ccaaagtaag cgctatgaac acgaatttga tgtgtccgtc ctgttttagg agaagcctcc
    18061 acccaacagg cctgatgata attttccagc aatttaaaat ctgtttctga tgccttacca
    18121 tctttttgaa cggaaacaac cctttctccg gatttcaatg tgttcttctc gagaggagct
    18181 gtaattgtaa ttttcttttt accgtcccaa cgatgcgtta ataaagccca ataaattttc
    18241 tgcacttcgc gggcttccag taatgcttga atggctcgta acgtgcttct ttttttggct
    18301 aataaaagac aaccggaagt ctctctatcc aatcgatgta ccaattccaa ataggataaa
    18361 tcgtgccttg tctttcttaa tgcttcaata acccccaggc ttaaaccact gccaccatgt
    18421 actgcaatac ctgctggttt attaattacc aacaaggaag aatcttcaaa aatgatacta
    18481 tccttcaagc gttttgccaa attatcgctt acaaaaattt ctttagaatc agtcattcga
    18541 attggcggta ttcttatact gtccccaatt tgtaaacgac tgctagcttg agctcttttt
    18601 ttattaatcc gaacttcgcc gccacgaata atcctgtaga tatggctttt aggaactcct
    18661 tttaaaatgc gaatcagata attatccaaa cgctggccat tctcttcgga gccaatttct
    18721 ttataactta cttcactcat tgtcaataaa cctgttttct gagcgtgatt tttttgatat
    18781 gattagatat cgtacgggaa aatccctgta cg
//
LOCUS       DACTJE010000050        18781 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.050, whole genome shotgun sequence.
ACCESSION   DACTJE010000050 DACTJE010000000
VERSION     DACTJE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18781
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.050"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1045
                     /gene="legK2"
                     /locus_tag="I9760_001352"
     CDS             <1..1045
                     /gene="legK2"
                     /locus_tag="I9760_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegK2"
                     /protein_id="HAT5043125.1"
                     /translation="LENLHNKGYAHGDLTFKNILYNEKNNQLKIIDAGEMKVADERFW
                     AQDISIAIEMISLLFTTNETNQFKASRYWSDEPTVRRIVNPNPVEAKLNRILNSEYRC
                     VSDLKLAIERNYNEYLNNSNLVLKKNVELESEALGLQAYKNQMSKQQLLGEIQGFKEN
                     YWNMKDLLTLTNRHHLRVFLEYLDNICSAFKDDKTDEKSARAAYDFLNAQINKLFEDN
                     SKNSKPSFESFSEDVQRFLIHIDTYLMKNPSACSNSIASTIQLLKQLDNKKSFNPEQS
                     FKDFCSYKEITIQLLLKPFETPVAEMASAPAIASDLIEKQSDTKRKLQEMKSSANQDK
                     QPIFTPSLMRGPS"
     gene            1404..2717
                     /locus_tag="I9760_001353"
     CDS             1404..2717
                     /locus_tag="I9760_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAT5043126.1"
                     /translation="MEYHRVWRFSYGLVLLLLYASLVSAGNALTLKEVTQIALANNKD
                     LKAARYNVSIAKARLLQAGFWPNPSLNLSNNDDRLFTDEGEYSRSAGFSQAFPISGRI
                     AKQKTVARLDVLKAVAEIREAERQLSAKVASAFYTAVLTERRIQQVNYLLRINRELVD
                     VIHNRYHAAEISKLDDNAARIEYLRIVQEKHLLHSLSISQYATLNQLMGREANSSLSL
                     KMDFAAAKKLPALDVLKTQALKNRPDRQSIALSIERANADRRLAKAERFADWTVGLGI
                     QQDKIVVEEGPPQPADRTLGVTLSVPLPLLNRNQGRILEASHTGTQAMMSLRALDLSI
                     ATEIASNYEQLKALRKSLEETQRVSLQLTIENVKLARDSYENGQISFLNVLQVQKQQN
                     DLQTTYLNTLEKFLQVYVALCTAIGPGKTDGFCSYLEYQSNGDSK"
     gene            2714..3682
                     /locus_tag="I9760_001354"
     CDS             2714..3682
                     /locus_tag="I9760_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAT5043127.1"
                     /translation="MRLQLKIRMMIMTVFFLGLLSPIQVSFAHGGNIEVSEGGTKGPV
                     HLTPEQARMLDIKVVQATHRPMAQLLGLNGQIQLLPDAQADVSIRISGSVTAIDVNLG
                     DSVKTGQRLATVQSRLVGNPPPSVAVNAPIAGIIDARNINLGQAVEPNTVLFHISNRD
                     KLLVIAQVYEEDLGKVKVGQEVNVHVLSYPKQTFPGQVTLIEPNLDPLTRTVNVRITL
                     DNKEGLLKPGMFVRANVILSHNKAALVVPNAALLQADNEQFVFVQNGGTYKRVDVKVG
                     AVEDDYSEIIDGLVPGDLVVTQGNRELYTLWLSGGRKLQSGQEEHH"
     gene            3683..6883
                     /locus_tag="I9760_001355"
     CDS             3683..6883
                     /locus_tag="I9760_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAT5043128.1"
                     /translation="MFTRLIAWSLHNRLLILALTIILCLLGGYTLKQMPVDVFPEFAP
                     PQVVVQTQAPGMATQDVETLITYPLESAINGTPGVASVRSKTSVGLSTITVVFDDKTD
                     IYLNRQLVNERIQQVINRLPPGVSSPVMLPVTSAVGWLLKYALVSHTASPETLRTISD
                     WTIRPRLLALGGVASVVSLGGEVKQYQVQLDPKRMLAYGITVEEVRQALATGNQNVPG
                     AFVHQGGTEFVVSTIGRIKTLDDIKKTLIVVRKGVPVTVNNVATVAFGGEIKRGDASF
                     NAENAVIGTVSKIYGADTLTTTRKVEQALADIKKSLPAGVELNTEVFRQANFIESAIH
                     NLTRALIEGAVIVIAVLFLFLMNWRASFITFLSMPVSFIAGILVLHYFGFGINSMTLG
                     GMAIAIGEVVDDGIITVENVVHRLRLNRLAEKPLPTIEIVFDAVLEIRSSVVYATIII
                     SLVFLPIFFLSGIPERIFSPLAIAYIASVIGSLVVSITMVPALCYLLLVRKTEKNQDA
                     KVEVHALSEDERYHYAQEETVKQTQTETRFVIWLKKHFLTGLNWSLAHCKTVLVISIA
                     AFIFALSLLPFFGTSFLPEFHEGNFIIVMSTLPGTSLDESMRLGKQVSKTLLKYPQVI
                     SIAQRAGRSELDEDALPPNISEFDVHLNFGKDKTMSPDELLQSIRADLANIPGALFNV
                     GQFIAHRMDEVLSGVRAQVAVKLFGDNLATLNELGESVESILKTVPGVVDINKEQQIN
                     VPQLVIKIDREKAARYGVNIGQISEDIQILLNGVTVSSILEGQRTFDLYLRIGETERD
                     SVKAIQNMLIDAHGAANNQETKIPLRAVADIQLEPQPFAINRENVQRVLVIGFNVQGK
                     DLGSVIQEVQHQIKEKISLPGGYFVQYGGQFESQQQASRVIVLFGALAIFIMLVLLHK
                     AFGTFWEALLVMFNLPLALIGGVVSLFLASGDMSVAAMIGFITLFGIAARNGIILVSH
                     YNQLRSLGRGREQVVIQGTLDRLVPVLMTAATAALALIPLLWGSPAGKELQRPLAQVL
                     LGGLLTSTVLNMFVVPTVYNAFEIWHEKRKQSKESS"
     gene            complement(6985..8241)
                     /locus_tag="I9760_001356"
     CDS             complement(6985..8241)
                     /locus_tag="I9760_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5043129.1"
                     /translation="MENKKKRVVVATAGTFLEWAEFTYYAYIANIISGSFFPKLGNHL
                     GLIATFSVFALSYFFRPLGALFFGYLGDKMGRRVALQSSIMLMGLSSLFIGCLPAYNS
                     IGILAPILLLIFRCVQGFAVSGEFNGSAIYLIEHDSEKPCQAGSWTGFASALGMMFGG
                     LMSTLIYLPHMPEWAWRIPFLLGTLSCFCAMYFRKNLRESPPYLTISKEKPSNPLKIL
                     FDDYKKQLIKSVLLVAALGIYIYIMNVYYASHLAKYTTLSDAQTKFVVTLGQGLVVLF
                     IVLTGMYADRTDERHIIKIGLWGYFIAAPIAYLAPQTNSFALILLSQIPYAICNALVG
                     TPIFKLLNDFFPTQIRYSGVSIAWGVSMAIFGGTAPLVANYLQYSFKLATAPIFYILL
                     SASVALLVLHDKKNYKIAPIKLRSDN"
     gene            8465..10003
                     /locus_tag="I9760_001357"
     CDS             8465..10003
                     /locus_tag="I9760_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT5043130.1"
                     /translation="MQKANAKIQNINEIMFSDRVMLLNKNLLVSIPANFLCALIIYID
                     FNKTTTDQEMLSVWFMVGVTVFVLHGGLFLFNYYRSLPSKYLLKWLISVTAIYGALWG
                     IAGSVLIPQNDLLNQMIVIIIIIGIASGGLHILQPSFLASLLFFTLTLIPLCVWFFQQ
                     STLNYWFLGTALLIYFCFLLIISWMGYGLLNKNFKLRYENLDLINKLYAINANLEESE
                     LRFRSAFNSAAIGMAIVSLEGMWLKVNQSLCQIVGYSEEELLETNFQSITHPEDLELD
                     LGYVRQLLEGDIRFYHMEKRYIHKNGSVIWILLSASLIRDLDNKPLYFISQIQNIDAQ
                     KRAEQELQHIAYHDTLTGLGNRKLLELSFDQALAHAKRHQTQIAIMFMDLDYFKNVND
                     KLGHDIGDLLLVEIGKRLQTILRSTDLIVRHGGDEFIIALTELSNVNQVIEIANKILI
                     AVSKPITIKRYGISITGSIGISIYPYDGDDSDTLIRKADEALYRVKIGGKNNFQLFNT
                     VLHK"
     gene            10061..10564
                     /locus_tag="I9760_001358"
     CDS             10061..10564
                     /locus_tag="I9760_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HAT5043131.1"
                     /translation="MDINQYPSKVFKTEETGLLAKMIIKGLNSVPPSTYIALDASIYT
                     TTRNPQMYSLSTQLGQFKSSHVITPEIAFREQNGMGLAWIAARIGDIALISELAKHQA
                     ELNKADNDGFTTAHIAALTGQPLVLDELIKLGADLSPSLISAIHWKKNLLETRCCFSN
                     EFISAVS"
     gene            10539..10748
                     /locus_tag="I9760_001359"
     CDS             10539..10748
                     /locus_tag="I9760_001359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043132.1"
                     /translation="MNSFLQFHKGKQEVLLSPGELAVIMGDENHIQRYLDKVIEPHFR
                     SANRADCAEKRNTRAAIAEGSDCMS"
     gene            10780..11076
                     /locus_tag="I9760_001360"
     CDS             10780..11076
                     /locus_tag="I9760_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043133.1"
                     /translation="MLELQFEETDALLEAIKENEERVIGRKYYIDFIDEHKESYTNVM
                     WHISNPVKEELFQNEAQLRHSGLSTTVLYGMSWATSFLSSCYRFLTPKRPTRIS"
     gene            11084..11746
                     /locus_tag="I9760_001361"
     CDS             11084..11746
                     /locus_tag="I9760_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043134.1"
                     /translation="MPQTLDSQSKALFKKLLQEHKDSLKKEMARSEQFITNINKKLFP
                     ESEEMLPLFSTLSSKNLQLLPIYEFRIMKSRLAEDSDILKEPIDAQNLMHKKSSMSIL
                     RIPATEREENIYAVFQMLECPLMCNEDSLIPKILKEIKAIIKEIDPQEEQSISDALRL
                     IRNVIKDGARQERPLVAEVLKIFDATGSLNFRQIRNSLEHLPGLNEFSLSSRRPLSGH
                     ST"
     gene            complement(11779..12267)
                     /locus_tag="I9760_001362"
     CDS             complement(11779..12267)
                     /locus_tag="I9760_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAT5043135.1"
                     /translation="MKIRAIFFMIAIFFPVTLFASNTTCPLSNGINVLTKKRILNICK
                     NGTVVKTFKVALGYKGVGKKKAGDNKTPIGLYGLAHPRKSNQFKVFIPILYPTKKQLA
                     AGYSGRDVGIHGPAQTSNLFGWLNNLPGSTRGCVAVGKNNYIEYVANWVKANPGAKVL
                     II"
     gene            12745..14622
                     /locus_tag="I9760_001363"
     CDS             12745..14622
                     /locus_tag="I9760_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAT5043136.1"
                     /translation="MMNESSTEKKNELSLSFAALGVVFGDIGTSPLYAFGQVIKYFPI
                     NDHNIYGILSLIFWSLIIIVSIKYLVIVFRADNDGEGGIIALAGLIRQKIKKPGGWLL
                     FITLVGIGLIIGDGILTPAISILSAVEGLESLSPNLAKYVLPVTLIILFFLFKMQSIG
                     TGKIGIYFAPVMLIWFITIGVLGFLQIIQNPKVLMAINPYYAIYFFMIHKYFALFILG
                     GVFLVMTGGEALFADLGHFGKKAIRTGWFAVALPALLLCYFGQGAFVLMHIEYIKYPF
                     FSLSPDWFLPVMIILATIATIIASQAIISAAFSILKQASLLNLIPRLKIIYTSKFEKG
                     EVYLPLINFILALGTCSLVVIFKSSSNLADAYGIAVNLDMLITTVLVGIIAYYCWNWH
                     AFKVMIFPLILVIELAFFAGNIPKLLTGGWIPILIAFLGFVVMYTWHCGFEKLRELHH
                     RDALMDAFIIDELNQNKISRQSGMGLYIIDPYDCEGESLLHHLRLNRIFFENMIFVSI
                     KIENKPYIPIEDKFELIKKAEGFYLIFIHYGFTENINLPNELDEMFKRVYLPFEIIKN
                     KLIYFIEIVFVEMTRERQKHMYMWQKHLFSLMIRNAVPDIQFYRLPYNKTIAIGTYYQ
                     L"
     gene            14672..15133
                     /locus_tag="I9760_001364"
     CDS             14672..15133
                     /locus_tag="I9760_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HAT5043137.1"
                     /translation="MNKADLHNSVLFKGIDEKYIDDFLENCEEVELPEGEFLFHQNEI
                     GDSMYIVEQGELQIILDQNSTGSNPQEEQVIGVLKSGALFGELCVFGQLKRAASIRAL
                     VDCRLLKIEGEDFRIRIYSKDLDALLICYNIAKLLSQRFITLLSLRPTLNM"
     gene            complement(15301..15588)
                     /locus_tag="I9760_001365"
     CDS             complement(15301..15588)
                     /locus_tag="I9760_001365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043138.1"
                     /translation="MKVQQNELLILVIDFLEQHYHLNQIDATLVNGFSTLGYMVGAPF
                     FGVLSHKVKNVIPIMFYAALIESILLAFNILYLHDLHLKMIFYAIVGFLAS"
     gene            complement(15644..16555)
                     /locus_tag="I9760_001366"
     CDS             complement(15644..16555)
                     /locus_tag="I9760_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAT5043139.1"
                     /translation="MTIQKGLHERRALKISIAVTFLLAVVGILFGLLSGSLAIVFDGM
                     FNMVDTVISILAFFVARLLTSKGNRRFQYGYWHIEPMVLVLNGSILILLCTYALVNAI
                     GSLMSGGHELNFDWAFVFALLVFFLSTGMYFYLVKTNRKIKSEFLRLDIQSWLMSALI
                     STSLLLAFGIAALLHGGAYGYFTPYIDPLILAILTAFLIFVPMAAVRDAMRDIFRMAP
                     VGLDERIREFLDELIKQHDFKTYTSYVAKIGRAQFIEIHIVVPMDYPISSIETLDKIR
                     NEIALAIGEDTPQRWLTIAFTANENWI"
     gene            complement(16712..18118)
                     /gene="dbpA"
                     /locus_tag="I9760_001367"
     CDS             complement(16712..18118)
                     /gene="dbpA"
                     /locus_tag="I9760_001367"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAT5043140.1"
                     /translation="MTQSEHNDQHLSFSQFPLRQELIKSLASSNYENMTPIQMQSLPI
                     ILRNEDMIAQAKTGSGKTAAFALCLLNNLKISFFVVQALVLCPTRELAEQVSQAIRRL
                     ACLMPNVKIINLSGGIPMQPQLDSLRHGAHIIVGTPGRILKHLKNASLDLSQVKALVL
                     DEADRMLDMGFFDDIKNIISVCPKQRQTLLFSATYPEEIKRLSKQFMKDPKEVHVVTP
                     PEEIDIEQHFYEVSRHAQKFPLLKSLLLHYQPVSVLIFCNTKQQTIEVTDQLINEGFS
                     AIALNGDMEQIERDLAVLRFANQSCSILVATDVAARGLDIKELSAVINFDLAFDHDVH
                     IHRIGRTGRAGSKGIALSIVTPADAQRICAIEDNYPLPIHWGNINELENHNTARLVPE
                     MVTLCIASGKKDKIRPGDILGALTKDAGLAGNTIGKINITAMYSYVAIHHSQADKAYQ
                     YLQSGKLKGRKVNVRKIN"
     gene            complement(18358..18567)
                     /locus_tag="I9760_001368"
     CDS             complement(18358..18567)
                     /locus_tag="I9760_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAT5043141.1"
                     /translation="MSKTVNGVVKWFNEIKGFGFIEQEGGPDVFAHFKEISSSGFKTL
                     TEGQRVQFIVTQGAKGLQAQNITAL"
BASE COUNT         5871 a         3293 c         3743 g         5874 t
ORIGIN      
        1 gttggaaaat ctgcataaca aaggctacgc ccatggcgat ttaactttca aaaatatttt
       61 atataacgaa aaaaataatc aattaaaaat tattgatgca ggagaaatga aagtagcaga
      121 tgaacgcttt tgggcacagg atatttcaat tgccatagaa atgatatcac tcttgtttac
      181 aactaatgaa acaaatcaat ttaaggcatc acgctattgg tctgatgaac ctacagtcag
      241 gcgaatagtt aaccccaacc cagtagaagc aaagttaaat agaattttga acagtgaata
      301 tcgttgtgtt tcagatttaa agttagctat tgaaaggaat tacaatgaat atctaaataa
      361 ttcgaatctg gttttgaaaa aaaacgttga actagaaagt gaagctttgg gtttacaagc
      421 atacaaaaat caaatgagca aacaacagtt gttgggagaa attcaaggat ttaaagaaaa
      481 ttattggaat atgaaagatt tgttgacctt gacaaatcga catcatctta gggtattttt
      541 ggagtattta gataatatct gttccgcatt taaagatgat aaaactgatg aaaagtcggc
      601 cagagcggcc tatgattttc taaacgcaca aatcaataaa ttattcgaag ataattcaaa
      661 aaactcaaaa ccaagttttg aaagtttctc agaagatgtt cagcgatttt taattcatat
      721 tgatacctat ttaatgaaaa atccctctgc ttgctctaat tccatagctt ccaccattca
      781 gttattgaaa cagttggata ataaaaagag ttttaatcct gagcaatctt ttaaggattt
      841 ctgttcatat aaagaaatta cgattcaact gttattaaag ccatttgaaa caccagtggc
      901 tgaaatggct agtgcgccag ctatagcatc agatttaatt gaaaagcaat ctgacaccaa
      961 aagaaaactt caagaaatga aatcttctgc aaatcaagat aagcaaccta tatttacccc
     1021 ttcattgatg cgaggcccaa gctaaaaaat gtcactgaag tgttagagca gcctgtcatc
     1081 tattatttca ctgctcttgt gtaattacat ttgctgggag atactaacag gacttacgaa
     1141 aaatcccaat ctctatgtta aaaaatgttt ttaatttggt catttggttt atctaaactc
     1201 cttgattaaa aacattttct gcctcgacct tggggttttt cgtaagtcct gaataaatca
     1261 tataacaata aaaagaaggt attaattgat gacttacttt cgaataaaac acagtccatt
     1321 actcttgatg tctgcacctg ccacagatta ttttttcgat tccatgttga ctgaagatag
     1381 atagattttt tgagctatcg tttatggaat atcatcgtgt ttggcggttt tcttatggat
     1441 tagtcctgct actgctgtat gcctctctgg tatcggctgg gaacgctttg actcttaaag
     1501 aagtaaccca aattgcttta gcaaacaata aggatttaaa agccgcccgt tacaatgtat
     1561 ctatcgcaaa agcccggttg ctacaagcag gcttttggcc taatccaagc ttgaatcttt
     1621 caaataatga cgatcgttta ttcactgatg aaggagagta ttcgcgcagt gctggcttta
     1681 gccaggcatt tcccatatca ggacgtattg caaaacaaaa gacagtggct cgtctggatg
     1741 tattgaaagc tgttgcagaa atcagggaag ctgagcgtca actcagtgcc aaagtggcta
     1801 gtgcctttta tacagcagtg cttacagagc gtcgcataca gcaagtcaat tacttattac
     1861 ggattaaccg ggaattggtt gatgttatcc ataatcgtta tcatgctgca gagatttcaa
     1921 aactggatga caatgcagcg cgcattgagt atttgcgtat tgttcaagaa aaacatcttt
     1981 tgcacagtct cagtattagt caatatgcga cactaaatca attaatggga cgtgaagcca
     2041 actcttcttt atcacttaaa atggattttg ccgctgcaaa aaaactcccg gcattagatg
     2101 ttctaaaaac acaggcgctt aaaaatcgcc ctgatcggca atctattgcg ttatcgattg
     2161 agcgtgccaa tgctgatcgc cgtttggcaa aagccgagcg ctttgcagat tggacagtag
     2221 gccttggtat tcagcaggat aaaattgtgg tggaagaagg cccccctcaa ccagccgatc
     2281 gtactttggg cgtcacactg tctgttcctc tccctctttt aaatcgaaat caagggcgaa
     2341 ttttagaagc aagtcataca ggtactcagg cgatgatgtc attacgtgct ctcgatttaa
     2401 gtattgcaac ggagatagcc agtaattatg agcaactcaa agcattacgt aagagtcttg
     2461 aggaaacaca acgtgtttct cttcaattaa ctattgaaaa tgtaaaattg gctcgtgatt
     2521 cttatgaaaa tgggcaaatt tcttttttaa atgtgctaca agtgcaaaaa caacaaaacg
     2581 atttgcaaac cacttatctc aatacgttag aaaaattttt acaagtctat gtggccttgt
     2641 gtacagctat tggtcctgga aaaacagatg ggttttgctc ttatttagag tatcaaagca
     2701 acggtgatag caaatgagat tgcagcttaa aattagaatg atgatcatga ctgttttttt
     2761 tcttggcttg ctttccccca tccaagtgag ttttgcacac ggaggaaaca tagaagtaag
     2821 tgaaggaggc accaaggggc cggttcattt gactccggaa caagccagga tgcttgatat
     2881 caaagttgtc caggcaaccc atcgtcctat ggcacaattg ctgggtttaa atggtcaaat
     2941 tcaattatta cctgatgctc aagctgatgt cagcattcgt attagtggca gtgtgacagc
     3001 gattgatgtg aatttggggg acagtgtcaa gacaggacag cgcttggcta cagtgcaatc
     3061 acgattggtg ggaaatcctc caccgagcgt tgctgtcaat gccccaattg ctggtatcat
     3121 tgatgcaagg aacatcaatt taggtcaggc tgtcgagcca aacaccgttc tttttcatat
     3181 cagtaatcgc gataaattat tagtcatagc tcaagtgtat gaagaagatt taggtaaagt
     3241 gaaggtaggg caagaagtga atgttcatgt cttaagttat cccaaacaga cattcccggg
     3301 gcaagtcact ttaattgaac ccaatcttga tccattgacc cgtacagtta atgtacgaat
     3361 tactttggat aacaaggagg ggctactgaa acccggaatg tttgtgcggg ctaatgtgat
     3421 tttaagtcat aataaggcag cactcgttgt gccgaatgcc gctcttttac aagcagataa
     3481 cgagcaattt gtttttgtgc aaaatggagg cacttacaaa cgagtcgatg ttaaagtagg
     3541 cgctgtggag gatgactact cagaaattat tgatggtcta gttcctggcg atctcgttgt
     3601 tactcaggga aatcgcgagc tttatacact ttggttgagt ggaggccgca aactgcaatc
     3661 agggcaagag gagcatcatt aaatgtttac tcgccttatt gcctggtctt tgcataatcg
     3721 tctcttgatt ttggcattaa cgattattct ttgtttgctg ggtggttata ctctaaagca
     3781 aatgccggta gatgtttttc ctgaatttgc ccctcctcaa gttgtagttc aaacccaagc
     3841 gccaggcatg gcaacccaag atgtggaaac attaatcact tatcctttgg aaagtgccat
     3901 caatgggact ccaggggtag ccagtgttcg ctcaaaaacc tccgtcggat tatcaacaat
     3961 taccgtggta tttgatgata aaacggatat ctatctcaat cgtcaactgg ttaatgagcg
     4021 cattcaacag gtaatcaacc gtctccctcc aggtgtttcc tcacctgtga tgctgccagt
     4081 cacatcggca gtagggtggt tgcttaaata tgctttggtg agtcacacag caagccctga
     4141 aaccttgcgc actatttctg attggactat tcgtccacgt ctattggctt taggcggagt
     4201 tgcttcagta gtttcactgg gcggagaagt aaagcaatat caagtacagc ttgatcccaa
     4261 gcgtatgctg gcttatggca tcacagtcga agaagttcgt caagcgttgg caacaggaaa
     4321 tcaaaatgta cctggtgctt tcgtacatca ggggggtacg gaatttgtcg taagtaccat
     4381 tggtcgtatt aagacgttgg acgatataaa aaaaacactg attgtagtcc gcaagggagt
     4441 tcctgttact gttaataatg tagccacagt cgcttttggc ggtgaaatca aacgcggtga
     4501 tgcttcgttc aatgcagaaa acgctgtcat tggtactgtt tccaaaattt atggagctga
     4561 tacattaact acaacacgta aagtggagca agctttagct gatataaaaa aatccttgcc
     4621 ggcaggtgtt gaattaaata cagaagtatt tcggcaagcc aatttcattg agtcagccat
     4681 tcacaaccta accagggctc ttattgaggg agctgttatt gtgattgccg tgctctttct
     4741 atttttgatg aattggcgag cttcatttat tacctttctc tctatgcccg tttcctttat
     4801 cgcaggtatc ttggtccttc attattttgg ttttggcatt aactccatga ctttgggcgg
     4861 aatggcgatt gccattggtg aagtggtgga tgatgggatt attactgtag aaaatgtggt
     4921 acatcgctta agactgaatc gtttggcaga aaaacctttg cctacgattg aaattgtttt
     4981 tgatgcggtt ttggaaattc gcagttcagt agtttatgcg accattatta ttagcctggt
     5041 gtttttaccc atcttctttt tgtcaggaat tcccgagcgg atttttagcc cattagccat
     5101 tgcttatatt gcttccgtaa taggctctct tgtcgtttct attactatgg taccagccct
     5161 atgttatctc cttttggtgc gaaaaactga aaagaatcag gatgctaaag tggaggtgca
     5221 tgctttatcc gaagacgaac gttatcatta cgcacaagag gaaacggtta aacagactca
     5281 aacggaaacg cgttttgtga tttggctaaa aaagcatttt ttaaccggtt taaattggtc
     5341 tttggcccat tgtaaaacag ttttagtcat atcaattgca gcattcattt ttgctttgag
     5401 tttacttcct ttttttggaa cctctttctt gccggagttt catgagggca actttattat
     5461 tgtgatgagt actcttcctg gaacttcatt ggatgaatcc atgcgtttgg gtaagcaagt
     5521 aagtaagacg ttattgaaat atccgcaagt aatttctatt gcccaacgag cgggtcgcag
     5581 tgaattggat gaagatgctt taccaccgaa tattagtgaa ttcgatgtac atctgaattt
     5641 tggtaaagat aaaaccatgt ctcctgatga attgttacaa tctattcgag ctgatttagc
     5701 gaatattcca ggagcgttat ttaatgtggg gcagtttatt gcccatcgca tggatgaagt
     5761 gttatcagga gtcagagcgc aagtagccgt gaaactcttt ggtgataatc ttgctaccct
     5821 taatgaattg ggtgaatcgg tagaatccat tttaaaaacc gtcccaggag tagtagatat
     5881 taataaagag cagcaaatca atgtacctca gctggtcatt aaaattgata gggagaaagc
     5941 ggctcgctat ggtgtcaata ttggtcaaat ttccgaggac attcaaatcc ttcttaatgg
     6001 tgtgacggta tccagtattc ttgaaggcca gaggacgttt gatttgtatt tgcgtattgg
     6061 tgaaacagaa cgggatagcg ttaaagccat tcaaaatatg ctgattgatg cccatggtgc
     6121 ggccaataat caagaaacaa aaatcccttt gcgtgcggtg gctgatattc agttagagcc
     6181 tcaaccgttt gcaattaatc gagaaaacgt gcaacgtgtt ttggttatcg gttttaatgt
     6241 gcaagggaaa gatttgggta gtgtgattca agaagtacaa catcagatta aagaaaagat
     6301 ttcgttacct ggtggttatt tcgttcaata tggtggccaa tttgaaagtc aacaacaagc
     6361 atctcgtgtg attgttcttt ttggtgcatt ggcaattttt atcatgttag tacttcttca
     6421 taaggctttt ggcacatttt gggaagcctt gttggttatg ttcaacttac ctttggcatt
     6481 aataggtgga gtggtctctt tattccttgc cagcggtgat atgagtgtgg ctgccatgat
     6541 tgggtttatt actctatttg gaattgcggc acgtaatggg atcattttag tgagccacta
     6601 taatcaatta cgttctcttg gccggggacg tgagcaagta gtgatccagg gaactctcga
     6661 tcgcctggta cccgtattaa tgacagcagc gacagcagct ctggccttaa tacctctctt
     6721 gtggggatct cctgctggta aagaacttca acgtcctttg gcacaagtgt tattgggagg
     6781 attattaact tcaacggttt taaatatgtt tgttgtgcct acagtctata atgcctttga
     6841 aatctggcac gaaaagagaa aacaatcaaa agaaagttct taagaagtac catgatgaat
     6901 cgaataattg aacctattaa gaagtaatcg tcaccaattg cctggatgct tgcacagaca
     6961 tgtgcttgca aaggctaatt ttattcaatt atctgatctt aattttatcg gggctatctt
     7021 gtaattcttt ttatcatgta aaacgagcaa ggcaacactg gccgataaaa gtatatagaa
     7081 tattggtgca gttgctaatt tgaatgaata ttgtaaataa ttggcgacca atggcgctgt
     7141 tcctccaaaa atagccatgc ttactcccca ggcaatagaa acaccactgt atcgaatctg
     7201 agttggaaaa aaatcattca atagcttaaa aattggtgta ccgaccagag cattgcagat
     7261 tgcataggga atttgtgata agagaattaa tgcaaaagag ttggtttgag gggccaggta
     7321 agctatagga gctgcaataa aataccccca taaaccgatt tttataatat gtctttcgtc
     7381 agttctatcc gcatacattc ctgttaaaac aatgaatagg acaaccaatc cttgccctag
     7441 ggtaacaacg aatttggttt gggcatctga caaagtggtg tattttgcca aatgagaagc
     7501 ataataaaca ttcataatgt agatataaat acctaatgcc gctactaata ataccgattt
     7561 aattaattgt tttttataat cgtcaaataa aattttcagt ggatttgatg gtttttcttt
     7621 acttattgtg aggtagggtg gtgattccct caaattttta cgaaaataca tcgcacaaaa
     7681 gcaggataag gttcctaaca gaaaagggat acgccatgcc cactctggca tgtgaggtaa
     7741 ataaattaaa gtagacatca atccgccaaa catcataccc aatgcggaag caaatccagt
     7801 ccagctaccg gcctggcatg gtttttctga gtcgtgttca atcagataga tagcgctacc
     7861 attaaattca ccagatacgg caaagccttg tacacaacga aagatgagca agagaattgg
     7921 agccaagata ccaatactat tgtatgcagg aagacaacca atgaacagag aggataaccc
     7981 catcagcata atagaagatt gcagggcaac tcgacgcccc atcttgtctc caagatatcc
     8041 aaaaaagagg gctcctaacg gacgaaaaaa gtaactgagc gcgaaaacag aaaaggttgc
     8101 tatcaaacca agatgatttc ccaattttgg aaaaaacgat cctgagataa tattggcgat
     8161 ataggcgtaa taagtaaact cagcccattc cagaaaagtt ccggcggttg cgacaacgac
     8221 ccttttcttc ttattttcca tacaataaat caatagaata ttaaagtgat gtagagggat
     8281 tttaccttaa attgatctca ttattctatg taattcgatg atataacaga ttcatagagt
     8341 tactatagtg tcacattctg cttctaacaa ttaagtattt ttgtactata ttaatacaat
     8401 atattaatac aatagggttc aaattgaggt tttgaatttg tcactttatc tcagtgagtg
     8461 ctgaatgcaa aaggccaatg caaagattca aaatataaat gaaataatgt tttcagatcg
     8521 cgtaatgtta ctaaataaaa atttattagt gagcattcct gcaaattttt tatgcgcatt
     8581 aattatttat attgatttca ataaaacaac aacagatcaa gaaatgctct cagtatggtt
     8641 tatggttgga gtaacagtat ttgttctcca tggaggttta tttttattta attattatcg
     8701 ttccttgcca tccaaatacc ttttaaaatg gttaataagc gtcactgcga tttatggggc
     8761 tctatgggga atagcaggtt ctgttcttat tcctcaaaat gatctgttaa atcaaatgat
     8821 tgtcataatt ataattattg gaatcgcatc aggtggatta catattcttc agcccagttt
     8881 tttagcaagt ctattatttt ttacattaac ccttattcct ctatgtgtct ggttttttca
     8941 gcaaagtaca cttaactatt ggttccttgg aactgcattg ctgatttact tttgtttttt
     9001 attaattata tcctggatgg gatatggact tcttaataag aattttaaat tacgttatga
     9061 gaatttggat ttaattaata aattgtatgc aattaacgct aatttggagg aaagtgagtt
     9121 acgtttccgt tccgcattta attctgctgc tattggtatg gctatagttt ctttggaggg
     9181 gatgtggtta aaggttaatc aatcgctttg tcagattgta ggatactcag aagaagaatt
     9241 attggaaact aattttcaat cgatcactca tccagaggat ttagagctcg atttgggtta
     9301 tgtgaggcaa ttattagagg gggatataag attctatcat atggaaaaac gctatattca
     9361 caaaaatggt agtgtcattt ggattttact cagtgcttct ttaattcgag atctcgacaa
     9421 taaaccactc tattttatct cgcaaattca aaacattgat gctcaaaaac gagcagagca
     9481 agaacttcaa cacatcgcat atcatgacac tttaactggt ttgggaaata gaaagctatt
     9541 agagttatca tttgaccagg cattagctca tgcgaaacgt caccaaacgc aaattgctat
     9601 tatgtttatg gatttggatt attttaaaaa tgtgaatgac aaattgggtc atgatattgg
     9661 ggatctttta ttagttgaga ttgggaaaag attacagacg attttaaggt ctactgatct
     9721 aattgtcagg catggtggtg atgaattcat tatcgcgctt actgaattat ccaatgtaaa
     9781 tcaagttata gaaatagcaa ataaaattct aattgctgta agcaagccta tcacaatcaa
     9841 acgttatggt atttcaatta caggcagtat tggtataagt atctatccat atgacggcga
     9901 tgattcagac acattaatta gaaaagctga tgaagcatta tatagggtga aaataggggg
     9961 aaaaaataat tttcaattgt tcaataccgt gttacataag taagactctc ttgatcatac
    10021 tctggcattg acttataaac ctggtaatgg gtgggggttt atggatatta atcaataccc
    10081 ctctaaagtt tttaaaaccg aagagacagg attacttgct aaaatgataa taaagggttt
    10141 gaattcagtc ccgccttcca cttatatcgc tttggatgca tccatttaca ccacaacaag
    10201 aaacccacaa atgtattcat taagcacaca attgggtcaa tttaaatcca gtcatgtaat
    10261 caccccggaa atagcattcc gggaacaaaa tgggatgggt ttagcctgga ttgcagcacg
    10321 aataggagat attgcactta tctcagagct tgcgaagcat caggctgaac tcaataaggc
    10381 agataatgat ggctttacca cggctcatat agctgcatta acaggtcaac ctttggttct
    10441 tgatgaatta ataaaattag gtgctgatct aagtccttcc ttaatttcag ccattcattg
    10501 gaaaaaaaac ctacttgaaa caagatgctg tttctcgaat gaattcattt ctgcagtttc
    10561 ataaaggaaa acaagaggtg ttactctcgc ctggagaact ggcagttatt atgggggatg
    10621 aaaatcatat tcaacgttat ctggataaag tgattgaacc tcattttaga tctgccaaca
    10681 gggcggattg tgcagaaaaa aggaatactc gggcggccat tgccgaggga agtgattgca
    10741 tgtcttaacc agctcattga tttaaaacgc caaaaggaaa tgcttgagtt acaatttgaa
    10801 gaaacagacg cgcttctgga agcaatcaaa gaaaatgagg aacgcgtcat aggcagaaaa
    10861 tattatattg attttattga tgagcacaaa gagtcctata ctaatgtcat gtggcatata
    10921 tccaaccctg taaaagaaga gctgtttcag aatgaagcac agcttcgtca ctcaggactt
    10981 tcaactacgg tactttatgg catgagttgg gcgacgtcat tcctttcctc ctgctaccgt
    11041 tttttgacac ccaagcgtcc aacaagaata tcttgactct ttcatgcccc agactttgga
    11101 tagtcaaagt aaagccttgt ttaaaaagct tttgcaagag cacaaggata gcctaaaaaa
    11161 agagatggcc aggtccgagc aatttattac taatatcaat aaaaaattat tccctgagtc
    11221 agaagagatg ctccctctat tcagtacgtt gtcgtcaaaa aacctgcagc tactgcctat
    11281 atacgagttt cgtataatga aaagcagact tgcagaagac agtgatatac taaaagagcc
    11341 tatagatgca cagaacttga tgcataaaaa atcatcgatg agtattctaa gaattccggc
    11401 aactgagcgt gaagaaaata tttacgcggt atttcaaatg ctagagtgcc ctctaatgtg
    11461 taatgaagac agtttaattc ccaagatttt aaaagaaatc aaagcgatta taaaagaaat
    11521 agacccacaa gaagagcaat caattagtga tgcgcttcgc ctgattcgca atgtgataaa
    11581 agatggggcc cgacaagaga gacctttagt tgctgaagtt ctaaaaatat ttgatgctac
    11641 gggtagcctt aattttagac aaatacgcaa ttcgttagag cacttacctg gtttaaatga
    11701 attttcttta tcatcaagac gaccactatc agggcattct acataaaata aataggcggt
    11761 atcaaatttc gtctcaattt aaataattaa aaccttagct ccaggattgg cttttaccca
    11821 atttgccaca tactcgatgt agttgttttt gccaacagca acacatccac gagttgagcc
    11881 tggtaagttg tttaaccaac caaataaatt ggatgtctgg gctggtccat ggatcccaac
    11941 atccctacct gaatatccag cagctaattg ctttttagtc ggatagagaa ttggaataaa
    12001 gactttaaat tgattggatt ttcttggatg ggctaaccca tataaaccaa ttggagtttt
    12061 gttatcccca gccttttttt taccaacgcc tttgtacccg agcgctactt taaaggtttt
    12121 aacgacggtg ccgtttttac aaatatttaa aattcgcttt ttagtgagta cattgattcc
    12181 atttgataaa ggacaagtcg tattactagc gaataaagta actggaaaaa aaatagcgat
    12241 catgaaaaaa attgctctta ttttcatata aataggctaa tattgttgaa gttgggcaat
    12301 aattatccca caatattgtt tttattcata gacctggcag gctaaatctt atgtaaatat
    12361 acgtagcttg aaagtctgtt taggaaaaaa tcaatttcta tggcgtgctc tgaagaactc
    12421 tccttcagaa atcttagttc gaagccaagt attttagcca tgacaaactt taaaataaag
    12481 atttgatgag acggtgatta atgataacta agaagatctg gaacaatttt aaaaaaaact
    12541 gagaaaatat atcaaaaaac ttgaaagatg tatgggccta ttgcttatgc aatgaagaaa
    12601 gcattttaca gttggcgata gatagaaata aataattcac caggcacagg atacttggag
    12661 ataatgtttt tttaatatca gatagtacta tactcaaaat agtaattcat aaaggatctt
    12721 aaattatatt gggagtaacc ccggatgatg aatgagtctt caacagagaa gaagaacgag
    12781 ctcagcttat catttgcggc tctgggggtt gtttttggtg atataggaac aagtcctctt
    12841 tatgcatttg gccaagtaat aaagtatttt ccaatcaatg accataatat ttatggtatt
    12901 ttatcgctca ttttttggtc gctgatcatt atagtcagta ttaagtatct ggtaatcgtg
    12961 tttcgtgctg ataatgatgg cgaaggcggt attatagctt tagcagggtt gataagacag
    13021 aaaataaaaa aaccaggagg ctggctttta tttattactc ttgttggcat agggcttatc
    13081 attggtgatg gaatattaac tccagcgata tccatattaa gtgctgttga aggtctggaa
    13141 tctttgtctc ccaatttggc caaatatgtt cttccagtga cattaattat tttatttttc
    13201 ctctttaaga tgcagagcat aggtactggg aaaattggta tttatttcgc tcctgttatg
    13261 cttatttggt ttattaccat aggcgttttg ggttttttac aaattataca gaaccccaaa
    13321 gtactgatgg caataaatcc ttactatgcg atttattttt ttatgataca taaatacttt
    13381 gctcttttta tattgggagg ggtgttttta gtaatgactg gtggtgaggc tttatttgct
    13441 gatttagggc attttggcaa aaaggccatt cgaacagggt ggtttgctgt ggcgcttccc
    13501 gcattacttt tatgctattt tggccaggga gcgtttgtat tgatgcatat agagtatatc
    13561 aaatatccct tttttagttt atcgccagat tggtttctcc ctgtgatgat tattttggct
    13621 accattgcaa ccattattgc atcacaggca attatttcag cggcattttc gattttaaag
    13681 caggcgtcac tgcttaatct gattccacgt cttaagatta tctatacgtc caaatttgag
    13741 aaaggcgaag tatacttgcc acttatcaat tttattttag ctttaggtac atgttcactt
    13801 gttgtgattt ttaaatcatc atctaatctt gccgatgctt acggcatcgc tgttaattta
    13861 gatatgctta tcacaacagt tttagttggg atcattgctt attattgctg gaattggcat
    13921 gcgttcaagg taatgatttt ccctctgata ttggttattg aacttgcatt ctttgctgga
    13981 aatataccca agttgttaac tgggggttgg atccctattt taatcgcgtt tctaggattt
    14041 gtagtgatgt atacctggca ctgtggcttt gagaaactac gggaattgca tcatagagat
    14101 gctctgatgg atgcttttat cattgatgaa ttaaatcaaa ataaaatttc aagacaatcc
    14161 ggaatgggat tatatattat cgacccctat gattgtgaag gggaaagcct tcttcaccat
    14221 cttcgcctca accgtatttt ttttgagaat atgatttttg taagtatcaa aattgaaaat
    14281 aaaccttata tccctattga agataaattt gagcttataa agaaagctga agggttttat
    14341 ttaattttta ttcattatgg gtttactgaa aatattaatt tgcctaatga attagacgaa
    14401 atgtttaaaa gagtatactt accttttgag ataattaaga ataaattaat ctattttatt
    14461 gagatcgttt ttgttgaaat gactagagaa aggcaaaaac acatgtacat gtggcaaaag
    14521 cacctttttt ctctcatgat acgcaatgct gtgccagata ttcagtttta tcgattacct
    14581 tacaataaaa ctattgcaat agggacttat tatcaattat gagcccattc aagaggcgat
    14641 attgggtttt taagtggtta aggatagtga aatgaacaaa gccgatttac ataacagcgt
    14701 gttatttaag ggaatagatg aaaagtacat tgatgatttt ttggaaaatt gtgaggaagt
    14761 tgaacttccg gaaggtgaat ttctttttca tcaaaacgaa attggtgact caatgtatat
    14821 tgtcgaacag ggtgaattac aaattatact ggatcaaaat tcgacaggct cgaatcctca
    14881 ggaggagcaa gtgattggag tacttaaaag cggagcattg tttggggaat tatgtgtatt
    14941 tggacaacta aaacgagccg catcaatccg tgccttagta gattgtagat tattaaaaat
    15001 cgaaggggag gatttcagga ttcgtatcta ctcaaaagat ttggacgcat tgttgatttg
    15061 ttataatatc gcaaaactat taagccagcg ctttattact cttttaagct tgcgtcctac
    15121 tctgaatatg taaattggaa tagatctcat taaatgacaa gctcaaatag attcaaaccc
    15181 acggaaccat agttcgaagc tgatgctctg ataattcctt ataaatcaat atattgtaag
    15241 ctgtcaaatt cacctgaaat ggcttattac cgcgaggtgt cgctattatg gagtctgtta
    15301 tcaactagct aaaaaaccaa ctatggcata aaagatcatt tttaagtgta ggtcatgaag
    15361 ataaaggatg ttaaatgcca ataaaatact ttcaatcaat gcggcataaa acatgattgg
    15421 aatgacgttc tttactttat gagacaacac tccaaaaaaa ggtgcgccga ccatataccc
    15481 caaagtggaa aagccattaa caagagttgc atctatctgg ttaagatgat aatgctgttc
    15541 tagaaagtca attactaaaa ttaagagttc attttgctgt actttcaatt ttcagtagca
    15601 tgttttcaca aatatcctta tggaaacttg aataaaacgc ttgttaaatc cagttttcat
    15661 ttgcagtaaa ggcaatggta agccaacgtt gtggcgtatc ttctccaata gccaacgcga
    15721 tttcattacg aattttatcc agggtttcaa tgctcgaaat tgggtaatcc ataggtacta
    15781 ctatatgtat ttcaataaat tgtgcgcgtc ctatttttgc aacgtagctt gtataagtct
    15841 tgaaatcatg ctgtttaata agctcatcta aaaattctct tattctttca tcaagaccca
    15901 caggagccat gcgaaatatg tctctcatgg catcacggac tgcggccata ggcacaaaaa
    15961 tcagaaatgc agtaagaatt gctaaaataa gggggtcaat gtaaggggta aaatatccat
    16021 aagccccacc atgcaacaag gctgcgatgc caaatgccaa taggagagaa gtagaaatca
    16081 aagcagacat taaccaactt tgaatgtcta gtcgcaagaa ttcggactta attttgcggt
    16141 ttgttttgac taaataaaaa tacatgcctg ttgataaaaa aaacaccaaa agtgcaaaca
    16201 caaatgccca atcaaaattg agttcatgtc caccagacat caaactgcca atcgcgttta
    16261 ccagtgcata agtacaaaga agtattagga tactaccatt caaaacaaga accataggtt
    16321 caatatgcca gtaaccatat tgaaatctcc gattcccctt acttgttaaa agacgggcaa
    16381 caaaaaaagc aaggattgat ataacggtat ccaccatatt aaacatgcca tcaaagacga
    16441 tagccaatga accggacaat agcccaaata aaatacctac cacggctaag agaaaggtaa
    16501 cagcgattga tattttaaga gctcttcgtt catgaagtcc cttttgtatt gtcatttgag
    16561 tgatatcctg attttaacaa tgctttatct acttatacta ttgcttcaac agccactaat
    16621 aaatccatag gcttgtatta ttaggtcttt tacataataa ctgcttcttt tttcaattgt
    16681 tgcagtaagt ttttcgcatg gtttagaaaa atcaatttat tttacgtaca ttaactttgc
    16741 gtccttttaa ttttccactt tgtaaatatt ggtaggcttt atctgcttgg ctatggtgaa
    16801 ttgcaacata ggagtacatg gcggtgatgt taattttgcc aattgtgttg cctgctaatc
    16861 ctgcatcttt ggttaatgcc ccaaggatgt caccagggcg aattttatct tttttaccgg
    16921 aagcaataca gagtgtaacc atttctggca ctaagcgagc agtgttgtga ttttctaatt
    16981 cattaatgtt tccccaatga atggggagcg ggtagttatc ttcaatggcg caaattcgtt
    17041 gtgcatctgc aggcgttaca atgcttaagg caatgccttt actcccagct cgtcctgttc
    17101 gaccaatacg atgaatatgg acgtcgtgat caaaagcgag atcaaaatta atcactgcgg
    17161 aaagttcttt tatatcaagt cctcgtgctg caacatcagt ggcgacaaga atagaacaac
    17221 tttggttggc aaaacgcaag acagcaagat cacgctcgat ttgttccata tcaccattta
    17281 aagcgatggc actgaagcct tcattgatga gctggtctgt gacttctatt gtttgttgct
    17341 tggtattaca gaagattaac actgaaacgg gttgatagtg caacagtaat gattttaata
    17401 agggaaattt ctgagcatgt ctcgagactt cataaaaatg ttgttcaata tcaatttctt
    17461 caggaggggt taccacatga acttctttcg ggtccttcat aaattgtttt gaaagccgtt
    17521 tgatttcttc aggataagtc gcagaaaaaa gcagggtttg tcgttgtttg ggacaaaccg
    17581 aaataatgtt tttaatgtca tcaaaaaaac ccatatccag cattctgtct gcctcatcca
    17641 aaactaaagc ttttacctga gataagtcta aagaggcgtt ttttaaatgc ttaagtattc
    17701 tccccggtgt tcctacaata atatgagctc catgtcgtaa tgaatcaagc tgaggctgca
    17761 ttggtatacc accagataaa ttaataattt tgacattagg catcaaacag gctaatcgac
    17821 ggatagcttg gcttacttgt tccgcgagtt cacgggtagg gcaaagaacc aaggcttgta
    17881 ctacaaaaaa agaaattttt aaattattta ataaacacaa agcaaaagca gcggtctttc
    17941 cactccctgt ttttgcttga gctatcatat cttcatttct aagaattatg ggcaggcttt
    18001 gcatttggat aggggtcata ttttcataat ttgaagaagc aagacttttt attaattctt
    18061 gacgaagggg gaattgagag aatgataggt gttggtcgtt gtgttctgat tgagtcatgg
    18121 aaagtgctta ttgttataat aggtgaatag cttagcactt cttagtgcaa ttttaaatca
    18181 atttattttg ggcaagctta atctgatgct tacaattgtc ttttctactt gaatccgtgt
    18241 agatcaaaat aacattaaaa tcatctcaaa gacatcaaca gagagtgatt ttaatgtttc
    18301 tatcagatat atcattctac aaagagatat aagtaaaaat aatattagct acttaaatta
    18361 aagggctgtt atattttgtg cttgaagacc ttttgctcct tgcgtcacaa tgaattgtac
    18421 tcgctgacct tcagtcaggg ttttaaagcc agaacttgaa atttctttaa agtgagcaaa
    18481 tacatccggg ccgccttctt gctcaataaa accaaatcct ttgatctcgt taaaccattt
    18541 aacgactcca tttactgttt tagacataag tatccttaag tatttattat agtgcctgta
    18601 taggcgaatt tagccggaaa ccggtcaaga attaaaaact accgaacgag ggattaaatg
    18661 aataaaacga cgatttggaa agtataaaaa tagacatttt ctagctagga tcagaatact
    18721 attctttaag gctacaagtc aaggctctac cccaaataag atattggctc taccccaaca
    18781 a
//
LOCUS       DACTJE010000051        18599 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.051, whole genome shotgun sequence.
ACCESSION   DACTJE010000051 DACTJE010000000
VERSION     DACTJE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18599
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.051"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1221
                     /gene="legY"
                     /locus_tag="I9760_001369"
     CDS             <1..1221
                     /gene="legY"
                     /locus_tag="I9760_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegY"
                     /protein_id="HAT5043142.1"
                     /translation="KKHFLNNFQTNGAIVASPSQYNPNYYYDWIRDSAIAMGLVETWY
                     EASQSARYKKLLLEYVSWTEKIQHQADPIAGQDILGEPKFYINGNPFDGEWGRPQNDG
                     PALRASVLIRFAQQLLDHNEIDYVKSHLYNNTMDPQSMGTIKMDLEYIAHHWQDANFD
                     LWEEVYGHHFFTAMAQQKALTDGAILAHQLHDRQAAVFYEMQANLINSRLKQHLDHQN
                     KIIQATLLPHPGPQKTLELDSSVMLGILINPQKEGVFAPHHTFVQNTAKALHEQFNLM
                     FPINKNRSGAILFGRYPGDTYDGYQTNSIGNPWFILTATMAEYYFTMAHNLSPNSINK
                     LHIQNYLKKGDNYLRLIKQYGPDLNLSEQINLNTGVQQGATSLTWSYVSVLRAIHLRE
                     QLENRIKTTGWNTY"
     gene            complement(1311..2732)
                     /locus_tag="I9760_001370"
     CDS             complement(1311..2732)
                     /locus_tag="I9760_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAT5043143.1"
                     /translation="MAWIVAIIGSIAGFLFGYDEGIIAGSLGLVKNHFNLNATHIGVM
                     ASALPFGALFGSLLIGAFMASKCVKRFGRRSLLSFAGFLFFVGALGAGFAETVSVLIL
                     SRLILGLAIGMASVLTPLYLAETAAVQSRGAVVAIYQLALTVGIVCSYSVNYLLIEQQ
                     AWRAMFASSAIPALLLTLGILFMPESPRWLCSVGRHGAAANSLRKLRGKQSVEQELKD
                     IEATLANEPKQGNWLLLFQKPLLPVLMLGTILFCLQQLSGINVVIYFAPEIFKNLGLG
                     STTGQILATMGIGLVNLLVTIIAILYVDKLGRRKLLLLGFAGTSLSLFALSLFSLNHV
                     AWLSYLSVICLMVYIFSFAISVGPIPHIAMAEIFPLHVRGAGMGMSSMSNWLFNTIVI
                     FSFPVLHQMFGIEMTFVLYAVICFLGFIYAYIYMPETRNISLEQIETYIMSGKPLRFL
                     GREDEEVNAVSTKSESSLLASTN"
     gene            complement(2845..3507)
                     /locus_tag="I9760_001371"
     CDS             complement(2845..3507)
                     /locus_tag="I9760_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAT5043144.1"
                     /translation="MSFSEWKTQPGAVFAASPVIPVIVIKELEDALPLAEALFAGGIH
                     VLEVTLRTPVAIKALELLINTFPDELIGAGTVITPGQFHDVVAAGARFAISPGQTREL
                     LIAGQKSEIPLIPGVASVSELMEGLGMGYNHFKFFPAAAAGGIPMLKAISGVFPQVKF
                     CPTGGINSKNYEEYLCLPNVACVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCS
                     QN"
     gene            complement(3494..4501)
                     /gene="glk"
                     /locus_tag="I9760_001372"
     CDS             complement(3494..4501)
                     /gene="glk"
                     /locus_tag="I9760_001372"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAT5043145.1"
                     /translation="MNFFMMPHDLAQYAIVADIGGTFARFSRVNLVNLQMDKIEISPC
                     AEFISLESALLTYKNRHSLQEIKHIAIAIACPVIDDLVSMTNCHWQFSITELKQRLGL
                     EVLEVMNDFTAIAMSLPVLSTQDLVQIGNGYLDASKVRVVLGAGTGLGVAYLIPHQHH
                     YSAFAGEGGHADWGAKTEQEWFIYRYLKSKYSHVSYERLLSGQGLENLYQALAAYHSK
                     KVEFLSAAQIISLALNQECFIAHKAVAQFFSSLGSFAGDLALTYGAFGGVYIAGGIMP
                     RLLSLVHQSDFRIQFEDKGRFSDFNALIPTYVIAAAQPGILGASVSLKQILTGASHVL
                     F"
     gene            complement(4476..6314)
                     /locus_tag="I9760_001373"
     CDS             complement(4476..6314)
                     /locus_tag="I9760_001373"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAT5043146.1"
                     /translation="MHPVINQVTENIQNRSAARRAAYLNQIDKARTKGPQRSILHCGN
                     LAHGFAACAKQDKAILRGNAKANIAIVSAYNDMLSAHQPYAVFPDLIKEAISAAGGVA
                     QFAGGVPAMCDGITQGQPGMELSLLSRDVIALSTAVALSHNMFDGGLLLGICDKIVPG
                     LLMAALAFGHLPFIFVPAGPMPSGISNQEKARIRQLYAAGQVDKNALLDVEAASYHTA
                     GTCTFYGTANSNQLVIELMGLQLPGSSFVNPNTPLRNELTKAAAYQILSLTDLNSDYM
                     PIGQMLDVKNIVNGIIGLLASGGSTNHTMHLVAIAAMAGYWVNWDDFAQLSAIIPLIA
                     RIYPNGQADINHFQRAGGMAYFVKTLLEADLLHPDVNTVVGFGLDKYTQQPQLIDNRV
                     VWVNGPDSSSNNEVLTSATDPFKPQGGLQVLSGNLGRAIIKTSSLYDEHYVIEAPAVV
                     CSSQEEFEHLFNQGLLNKNCVVVVRFQGPKACGMPELHQLTPKLGVLMDKGFHVALVT
                     DGRMSGASGKVPAAIHVTPEAVDGGGIAKIETGDMILIDCERGILQLLVSDEELAGRS
                     PVAIEGYSSVLGMGRELFGNLRLHFTGAEQGACSLFKGNEFFYDAS"
     gene            complement(6302..7015)
                     /gene="pgl"
                     /locus_tag="I9760_001374"
     CDS             complement(6302..7015)
                     /gene="pgl"
                     /locus_tag="I9760_001374"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAT5043147.1"
                     /translation="MQFHNFRDAKQLIHDMVEKLRTILSDAIKRRGQAFLVVSGGRTP
                     IDLFKELALADLPWEKVVITLTDERCVNLFDPHRNELLVREFLLQEKAKKAKFISLFD
                     EKEDPLGHLNEVNSQIDSLPMFDAVVLGMGEDGHTASLFPCSDELSAGLDDKANAVLI
                     VKPRTASFQRVSLSKRRLLNSSTIFLHLVGQRKRVVLEQAMATNNPEILPICAILNAP
                     DANVQVMYAPSDEEKECIL"
     gene            complement(7002..8492)
                     /gene="zwf"
                     /locus_tag="I9760_001375"
     CDS             complement(7002..8492)
                     /gene="zwf"
                     /locus_tag="I9760_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAT5043148.1"
                     /translation="MLLKAESKYPCDLILFGTLGDLSCRKLLPALYQLELANLLHEKT
                     RIIGCAREELDFSAYVDLIKNKIQQFLFDEIDEAVWLRFIKKLAYCKLDLNQSDDYQK
                     LTDYVNPSERVVISYLAIPPSLYSQTCRSLARLNLTQKPSRVVLEKPIGHDLPSSVAT
                     NEQVACFFAEDQIYRIDHYLGKETVLNLLVLRFANSIFSTNWDNRVIDRVEISVAEEI
                     GVEGRWEYYDKSGQVRDMLQNHLLQILSLVAMEPPLSLSAESIRSEKLKVLKSLRFIN
                     KSNVHDHTVRAQYVANTLANGKKIPGYLEEVGANQASTTETFVAIKAYVDNWRWSGVP
                     FYMLTGKRLPKKQSEVVIYFKPQPYNIFHPLNEELYPNQLIIRLQPDEGVEVRIMNKI
                     PGLGERMMLHDSKLDLNFHHSFKTQRIADAYERLLLEVMLGNQYLFVSREEIEQAWQW
                     IDSIVSAWEEDNIPLYTYPACTWGPKEVIRLFNGQAHVWGDHNAVS"
     gene            8810..9055
                     /locus_tag="I9760_001376"
     CDS             8810..9055
                     /locus_tag="I9760_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043149.1"
                     /translation="MLNKRFSERLNKELDNIGVPEATAERIEILSKLIKIPKFKAEAL
                     LNGITSPDEAVLKTLAQELEVNADWLIGKSDSKSGAH"
     gene            complement(9059..9967)
                     /gene="rimK"
                     /locus_tag="I9760_001377"
     CDS             complement(9059..9967)
                     /gene="rimK"
                     /locus_tag="I9760_001377"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAT5043150.1"
                     /translation="MKIAILATNPHLYSHKRLKAEAEAAGHEVKIINPLYCYMNVAAS
                     NPKVHYRGGAPLPHFDAVIPRIGASITYYGTAVLRHMETMGMYTLNESIAISRSRDKF
                     RSLQLLARKGIPMPLTSFAQSPDDTEDLIHMVGGAPLVIKLLEGTQGKGVILADSHQS
                     AVSIINAFKEMHANILVQEFIEESRGTDIRCFVIGEKVVAAVKRQAKDGEFRANVHQG
                     GKAVKVKLSPQERAIAVSAAKTMGLRVAGVDLIRSNHGPLVLEINSSPGLEGIEKATN
                     INLAGKIIEYIEKKAKPISSNHRFHG"
     gene            complement(10334..10975)
                     /locus_tag="I9760_001378"
     CDS             complement(10334..10975)
                     /locus_tag="I9760_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HAT5043151.1"
                     /translation="MNIKIKGGAVTITVLALVSLLAGIYISQNFHFKKKINVADFHGT
                     YLENPRTVNNFSLTGTDQKVFDNSSLQGRWTLVFFGFTNCGYLCPTTMAELGKMYRIL
                     EKKGVKNLPQVVMISIDPQRDSLDKLGSYVTSFHKSFYGARGEEDSIKSMTHEMGIAY
                     AKINSKESSEPENYDIQHSGAVMLFNPQGELNAFFTTPHHADLLAKDYVLLVS"
     gene            complement(10985..11869)
                     /locus_tag="I9760_001379"
     CDS             complement(10985..11869)
                     /locus_tag="I9760_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAT5043152.1"
                     /translation="MRTEYAARLPVDWRDYVELCKPRVVLLMLLTVIVGMYLAAPGWV
                     SLRLIAFTLLGIGLCAGSAAAINHLVDRHIDSIMARTKKRPVAYGRVSVKQALWFAVI
                     IGLMGLSLLILFVNQLTALLTFVTLIGYAGVYTGYLKRATSQNIVIGGLAGAAPPLLG
                     WTAVTDQLDPQALLLVLIIFTWTPPHFWALAIYRYKEYQDAEIPMLPVTHGIQFTKLN
                     IYLYTVLLLVVSLLPFVVSMSGWIYLLGALVLGIRFLVWAHKLYFTDKPVVAMQTFRF
                     SILYLMLLFVFLLVDHYF"
     gene            complement(11859..12866)
                     /locus_tag="I9760_001380"
     CDS             complement(11859..12866)
                     /locus_tag="I9760_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HAT5043153.1"
                     /translation="MSNSSIRLAATLAVFLAFFVVMLGAYTRLTDAGLGCPDWPGCYG
                     RMVLPGADKGLQEAQSQYPDLPIESKKAWTEMAHRYAAGTLALLIFFIAFQVWFKRCQ
                     DQDLPWHLPLALFMLVVFQAALGMWTVTLKLLPVVVMGHLLGGMLIVACLCRFRLQIA
                     QLKGQNLPEWRPWLRLGIVIILLQIALGGWVSANYAGISCIGFPLCNGVWFPELHFTK
                     GFNLFSTIGANYQGGVLESEVRASIQFIHRIGAILTATYLLALSLIIVFKTHSIYLRA
                     TAIIMAMLVLLQFTLGIMNVIHLLPLKVAVAHNGVAALLLAVAFCSLHFTQDREVSHA
                     Y"
     gene            complement(12871..13416)
                     /locus_tag="I9760_001381"
     CDS             complement(12871..13416)
                     /locus_tag="I9760_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043154.1"
                     /translation="MKKPYKYMTLLLLVVIFAAPGITAYLFYKHPSWLGTSKVNKGIL
                     LSPPIALKPLDSSAKWRIIYWNPNGCDKTCLKQLNMLGRVRLSFGRKLYQVDEWLLLS
                     NKDSLIAEELKPILKEQDIHVAALSLTEMSKLSTLYPEAKIFIANPDNYLILSYQPGC
                     NPGDIYKDLKLLLNTTEIKSG"
     gene            complement(13400..14128)
                     /locus_tag="I9760_001382"
     CDS             complement(13400..14128)
                     /locus_tag="I9760_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HAT5043155.1"
                     /translation="MPSLTCFNFRFTSNWPMLILTAVCFFLFISLGFWQIHRADEKTA
                     MITAQQALAKQEPIIWQPGQKLPEQYQRISIEGAFLPKLFLLDNQHYQHQFGYDVVSP
                     MLLDDDSIVMVDRGWVSGDITRRTFPNVQTPNGKFKLFGMVYFPSKKQWVLGPSFEEK
                     ENKVTLLELIDEEILKQLLQKKVYPFIIRLDKNELFGFVREWEIVSMPPQRHFAYAIQ
                     WFAMALVILIIFVIFNLKKNEKTI"
     gene            14196..14399
                     /locus_tag="I9760_001383"
     CDS             14196..14399
                     /locus_tag="I9760_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HAT5043156.1"
                     /translation="MFTKIFIIFVMILIVASLASGLFYLVKDTDKSKRTVKALTVRIA
                     ISLSLFVFLFIAFKLGLIQPHGI"
     gene            14816..15364
                     /locus_tag="I9760_001384"
     CDS             14816..15364
                     /locus_tag="I9760_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HAT5043157.1"
                     /translation="MNKQMQWHLFGNTSGLLIICCLLILAFLDQFIFYELPCPICILQ
                     RICFIAVGLCFVMNLMLGIRASHYGLMLLATLLGLSISVRQMYIHLTPGDPGYGGLIF
                     GLHLYTWSAIIFLVIILLIAGALILDKGFMPDYKVRSKSAKVMIWVFFILILANGIST
                     FLECGPHECPDNPSKYYLLNNN"
     gene            15383..15826
                     /locus_tag="I9760_001385"
     CDS             15383..15826
                     /locus_tag="I9760_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAT5043158.1"
                     /translation="MRLLFISLILASGYLQADTINNYMNIANNIPQMEMKADPQAQAW
                     ARSARHVLTITSESIAETLIQANEAAKTQGKPIFCLPQGVQLNSFVMNELIQQTYKEI
                     SSQQSDKDKMTVSQVALLGLSKKYPCEPSPQEKQIQHVASLLSPQ"
     gene            complement(16039..16380)
                     /locus_tag="I9760_001386"
     CDS             complement(16039..16380)
                     /locus_tag="I9760_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAT5043159.1"
                     /translation="MSDKFSHITKDITTQLAKFRKEMPELMTGFSSLAQAATKDGALD
                     KKTKELIAMALAVAKQCPGCIGFHSQTLVKLQATREELLETLGMAVYMGGGPSLMYAA
                     EALEAFEEFSK"
     gene            complement(16580..17170)
                     /locus_tag="I9760_001387"
     CDS             complement(16580..17170)
                     /locus_tag="I9760_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0405 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043160.1"
                     /translation="MLYNLTRQVKFNNPSCLCNFQKMGYIDELLEELSARLPELEWKI
                     NGLSSSISIHNLPKGIFSSVIEFNGPACIKEIQDDIHALQKHKDESIACFLAERIQKK
                     INVLVVLCQMDKKNNKPESKISFDLTTLSTRQQWIKTMEEKICALEEQYQAMRKTLEQ
                     MKSCSNPATILHLQTELGNVEKRLTLAKETLNRAIS"
     gene            complement(17160..18563)
                     /locus_tag="I9760_001388"
     CDS             complement(17160..18563)
                     /locus_tag="I9760_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT5043161.1"
                     /translation="MSNKKHSLTIFSLTMITVGSVDSIRNLPATALFGSQLIFYFILG
                     ALFFLIPTALVSAELASGWARQGGVYIWVKEAFGKKSGFLAIWLQWIENVIWYPTILS
                     FVAGTIGYLINPSLTTNPYFLWAVIVSCFWGATIVNLRGMKSSAMFSNICSLAGLLLP
                     MSLIIGLGAVWIAQGNPLQISFDMPSIVPHLSDKGMWVSLTAIILSFCGIEIATVHAN
                     DVKDPQHAFPKALVYSVGIILSTLILGSLAIAVVLPQKDINLVAGIMQAFSAFFLKYH
                     MDWMMPVVAVMLVLGGLGGVSNWIIAPTKGLLVAAEEGNLPEVFQRTNGKGAPAMMLY
                     TQATIVTVLSALFLFMPSVNGSYWLLTALAAQLYMLMYLIMFVAAIKLRISQPYHPRP
                     FKIPGGMAGMLFVAGIGIIGVVTTLAVSFIPPEGINVGSATRYELTLIIGLLLMCAPP
                     FIASWLQAKEADLEPAV"
BASE COUNT         5945 a         4075 c         3293 g         5286 t
ORIGIN      
        1 aaaaaacatt ttttaaataa ctttcaaacc aatggagcca tcgtagcctc accctcgcaa
       61 tacaacccca attattatta tgactggatt cgagactcag ccattgccat gggattggtt
      121 gaaacatggt atgaagccag ccaatctgct cgatataaaa aacttttatt agaatacgtt
      181 tcatggacag aaaaaataca gcatcaagcg gatcccattg ctggccagga tatattgggt
      241 gaacccaaat tttatatcaa tggcaatcct tttgatgggg aatggggacg gccacaaaat
      301 gatggtccgg cactcagagc atctgtttta attcgttttg cgcaacagct tctcgatcat
      361 aatgaaatag attatgttaa atcacatctt tataacaaca ccatggaccc acaatccatg
      421 ggcactatca agatggattt ggaatacatc gcacatcatt ggcaagatgc aaattttgac
      481 ctgtgggaag aggtttatgg ccatcatttt ttcacggcta tggcacaaca aaaagcgctg
      541 accgatggcg ccattttagc tcaccaactt catgacagac aagctgctgt tttttatgaa
      601 atgcaagcca acttaattaa cagtcgactg aaacagcatt tggaccatca gaataaaata
      661 attcaagcca cattattacc tcaccccggt cctcaaaaaa cactagagct tgattcgtcc
      721 gtcatgttgg gtattttaat taacccacaa aaagaaggtg tttttgcccc acatcacacc
      781 tttgtgcaaa atactgcaaa ggctttgcat gagcaattta atttgatgtt tcctattaat
      841 aaaaaccgtt ctggagcaat attgtttggt cgttatcccg gcgatactta tgatggctac
      901 caaacaaaca gcatagggaa cccctggttt atcttaaccg ctacgatggc tgaatattat
      961 tttaccatgg ctcataatct gtccccgaac agcattaata aacttcatat acagaactat
     1021 ctaaagaaag gggacaatta cctaaggctg ataaaacaat atggccctga tctgaattta
     1081 agtgaacaaa ttaatttgaa taccggagtt cagcaaggag cgacatcctt gacctggagt
     1141 tacgtttccg tcttgcgagc tattcattta agagaacaat tggaaaatag aataaaaact
     1201 acgggctgga atacctatta ggattttata cgatagggta atgcgataaa gaatagaatt
     1261 tgtggcccgt gtcagtcaaa gataacacgg gcatcagagg aaaatttata tcaatttgtt
     1321 gaagccagca aagaagattc tgatttagtt gacacagcat taacctcttc atcctctcta
     1381 cctaaaaacc gtaatggctt gccactcatt atataagttt ctatttgctc taaactaata
     1441 ttcctggttt caggcatata aatataagca taaatgaacc ccaagaaaca gattaccgcg
     1501 tacagaacga aagtcatttc tataccaaac atttgatgca gaacaggaaa actaaaaata
     1561 actatcgtat taaacaacca attactcatg gatgacatcc ccataccggc accacggaca
     1621 tgaagaggaa aaatttctgc catagcaatg tgggggatag ggcccacgct gattgcaaaa
     1681 gaaaaaatat aaaccattaa gcaaataacg gataaataag atagccaggc aacatgattt
     1741 aatgaaaata gcgataatgc aaacaagctc aaactggttc ctgcaaaacc cagtaacagt
     1801 aatttgcgtc gccctagctt atcgacatac aaaatggcaa taatagtaac cagtaaatta
     1861 actaacccaa tccccattgt cgctaatatt tgtccagtcg tgctgcccaa gcctaaattc
     1921 ttgaatattt ctggtgcaaa ataaattacc acattgatac cgcttaattg ttgtaaacaa
     1981 aataatattg ttcctaacat caacacaggc aacaagggct tttggaatag aagtagccag
     2041 ttgccttgtt ttggttcgtt cgccagtgtg gcttcaatat cttttaactc ttgctcaact
     2101 gattgtttgc ctctcaattt tcttaaggag tttgctgctg caccatgacg tccaacgctg
     2161 cataaccacc tgggagactc tggcataaac aaaatcccca gggtcaataa caaggcaggg
     2221 atagcacttg aagcaaacat tgcacgccac gcttgctgct ctatcaataa ataattgacc
     2281 gaataagaac aaacaatacc cacagtcaat gcaagttgat aaattgcaac cactgcgcca
     2341 cgtgattgca cggctgctgt ttcagccagg tataatggag ttaaaactga agccattcca
     2401 atggccaaac ctaaaattaa ccgagataaa atcaacactg aaacggtttc agcaaaaccg
     2461 gcacccaggg ctccaacaaa gaacaaaaaa ccggcaaagg ataataaaga acgtctgccg
     2521 aaacgtttta cgcactttga tgccatgaat gcaccaatca acagcgaacc aaacaatgcc
     2581 ccaaaaggta aagcagaagc cataacgcca atgtgggttg cattcagatt aaaatgattt
     2641 tttaccagtc caagagaacc tgctattatt ccctcatcgt aaccaaataa aaaaccggca
     2701 attgacccaa ttattgcgac aatccaagcc atttttcact ccttgattta aattcttttc
     2761 ctctgtctct actctacaca atgaactgtt ttagctggca ctatccctta catcacataa
     2821 aaacgctttt tcaacccgaa tagattaatt ctgcgagcaa agttctcgct tctgagaact
     2881 caccgccata cataactctg taattaaaga ccagttatga ttcttgatag cctcttccgg
     2941 tacaatccag gaccctccaa cgcaagctac atttggcaag caaagatact cctcataatt
     3001 tttggaatta attccgcctg ttggacaaaa tttaacttgg gggaatacgc ccgatatcgc
     3061 tttcaacata gggatacctc cagccgctgc cgcaggaaaa aatttaaaat ggttatatcc
     3121 catccccaaa ccttccatta attcagagac acttgctaca cctggaatga gaggaatttc
     3181 acttttctgt cctgctatta ataattccct tgtttggcca gggctaatgg caaatcgagc
     3241 tcctgctgct accacatcat gaaactggcc aggggtaatc acagttccgg caccgattaa
     3301 ttcatctgga aacgtattga tcaataattc cagtgctttt attgccactg gtgttcttaa
     3361 tgtcacttca agaacatgaa ttcctccggc aaataaagcc tcggccaaag gtaacgcatc
     3421 ctctaactct ttgattacaa tgactggaat aaccggagat gcagcaaata ccgcgcctgg
     3481 ttgtgttttc cattcagaaa aggacatgag aagctcctgt caatatttgt tttaaactga
     3541 cgctcgcgcc taaaatacct ggttgagcgg ctgcaatgac ataagtagga attaaggcat
     3601 taaaatcact aaatcgtcct ttgtcttcaa attggattct aaagtcgctt tggtgtacca
     3661 aagagagtaa acgcggcata atccctcctg caatataaac accaccaaaa gctccataag
     3721 ttaatgctaa atcaccagca aacgatccca aacttgaaaa aaattgggct actgccttat
     3781 gagctatgaa acattcctga tttaatgcca aagatataat ttgtgctgct gacaaaaatt
     3841 caactttttt actatgatac gcggctaaag cttgatataa attttccagt ccttgtccag
     3901 acagcaatcg ctcataagat acatgggagt atttactttt caaataacga tagataaacc
     3961 actcctgctc agtttttgct ccccaatcag catgcccccc ttcccctgca aaagcactat
     4021 agtgatgttg atgaggaatt aaataagcaa cacccaaacc ggttcctgcg cctaacacca
     4081 ctctcacttt actggcatcc aggtaaccat taccaatctg caccagatcc tgagtcgata
     4141 atacaggtaa actcatcgca atcgcagtaa aatcattcat gacttccaga acttcaagac
     4201 caagtctctg ttttaactct gttattgaaa attgccaatg acaattcgtc atagaaacca
     4261 aatcatcgat gactggacac gcaatcgcta tcgcaatatg ttttatttct tgcaaagaat
     4321 gccggttttt ataagttaat agcgcggact caagactaat aaactcggca caaggagaaa
     4381 tttcaatttt atccatctgc aaattgacaa gattaactct actgaaacga gcaaaagtac
     4441 cgcctatgtc cgcaacaatc gcatactgcg ctaaatcatg aggcatcata aaaaaattca
     4501 tttcctttaa atagactaca tgccccctgt tctgcaccag taaaatgtaa tcttaaatta
     4561 ccaaacaact ctcgtcccat gcctagaaca gaggaatacc cttcgatagc tactggagaa
     4621 cggcctgcta actcttcatc agaaactaat aattgaagta ttcctctttc acaatcgatc
     4681 aaaatcatat cgcccgtttc aattttagca atcccgcccc catcaaccgc ttccggggta
     4741 acgtgaattg cagcgggaac cttgcccgat gcgccagaca ttcgaccatc agtaaccaat
     4801 gccacatgaa accctttatc cattagcaca ccaagttttg gcgttaattg atgtaattct
     4861 ggcattccac atgcttttgg gccttgaaaa cgaactacca ccacacaatt tttattaagc
     4921 aaaccttgat tgaatagatg ttcaaattcc tcttggcttg agcaaacgac tgcgggagct
     4981 tctatcacat aatgctcatc ataaagactg gatgtcttaa taattgctct gccaagattg
     5041 ccacttaaaa cctgcaaacc accctgaggt ttaaatggat cggtagcaga ggttaatact
     5101 tcattattgc ttgagctgtc aggaccatta acccaaacca ctcgattgtc aatcaattgc
     5161 ggctgctgcg tatatttatc cagtccaaaa cccacgactg tgttgacatc aggatgaagt
     5221 aaatcagcct ctagcaaggt tttaacaaaa taagccatgc ctccagctct ttgaaaatga
     5281 ttgatgtctg cttgaccatt aggataaatt cgagcaatta atggaataat tgccgacaat
     5341 tgtgcaaaat catcccaatt tacccaatac cctgccatag ccgctatcgc gacaaggtgc
     5401 atggtatgat tggtcgatcc ccctgaagcc aataatccga taataccgtt tacaatattt
     5461 ttcacatcaa gcatctgccc tattggcata taatcagaat ttaaatctgt taatgacaaa
     5521 atctgatatg ctgctgcttt ggttaattca ttgcgcaacg gggtattagg attcacgaag
     5581 gaagagcctg ggagctgcaa ccccatcagt tcaataacta attgatttga attggccgta
     5641 ccataaaatg tgcatgttcc agccgtatga taggaggctg cttccacatc caataaggca
     5701 tttttgtcga cttgacctgc tgcgtacaat tgacgaattc ttgctttttc ctgattggag
     5761 ataccagaag gcatgggccc cgcgggtaca aaaataaaag gcagatggcc aaatgccagc
     5821 gcagccatta ataatccagg gactatttta tcgcaaatac ctaagaggag ccctccatca
     5881 aacatgttat gtgacaaagc aacagcagtc gataaagcga tcacatcccg gctgagtaaa
     5941 cttaattcca tccccggttg tccctgagta atcccatcgc acatggctgg gacgcccccg
     6001 gcaaattgcg caactcctcc tgcagcagag atcgcttcct taatcaaatc agggaaaaca
     6061 gcatacggtt gatgcgccga taacatatcg ttataagcag aaacaatggc aatattggct
     6121 tttgcattac ctctcaaaat agccttgtcc tgtttggcac aagcagcgaa gccatgtgcc
     6181 aaattcccac aatgcagaat acttcgttgt gggcctttag tacgagcctt atcgatttga
     6241 ttcagataag ccgctcttct agcagcgcta cgattttgga tattttctgt cacttgattt
     6301 attacaggat gcattctttt tcctcatcgc ttggagcata catcacctga acattggcat
     6361 ctggggcatt taaaatagcg cagattggca ggatctccgg gttgttagtt gccattgcct
     6421 gctccagaac aacccgtttt ctttgaccta ctaaatgtaa aaaaatagta ctgctgttta
     6481 ataatcgcct tttcgataag ctaaccctct ggaatgaagc tgttctaggc ttcacaataa
     6541 gcaccgcatt tgctttatca tctaatcctg cacttaactc atcactgcat ggaaataaag
     6601 aagcagtatg gccgtcttcc cccattccta aaactactgc atcaaacatt ggtagagaat
     6661 caatttggct attcacctca ttcaaatgac ctaatggatc ctctttttca tcaaacaagc
     6721 tgataaattt ggcttttttt gccttctctt gcagtaaaaa ttcacgtacc agcagctcgt
     6781 tacgatgtgg atcaaaaagg ttgacacaac gttcatcggt caaagtgatc actacttttt
     6841 cccaaggcaa gtcggctaaa gccaattcct tgaacaaatc gataggggtt ctaccgccag
     6901 aaaccaccaa aaaggcttgc cctcttcgtt tgattgcatc tgatagaata gttcttaatt
     6961 tttccaccat gtcatgaatc aactgtttgg catccctaaa attatgaaac tgcattgtga
     7021 tcaccccaaa catgtgcttg cccattaaat aagcgtatga cttcctttgg cccccatgta
     7081 caagctggat aggtatacag cggaatatta tcttcttccc atgccgatac aatactgtca
     7141 atccattgcc aagcctgttc aatttcttct cggcttacaa aaagatactg attacctaac
     7201 atgacctcta gcaataatct ctcataggca tcagcaatcc tttgcgtttt aaaactatga
     7261 tggaaattta aatccaactt ggaatcatgc aacatcatac gctcaccaag acccggaatt
     7321 ttattcatta tcctcacctc caccccttca tcgggttgta agcgaataat taactgatta
     7381 gggtacaatt cttcatttaa gggatgaaag atattataag gctgtggttt aaaataaata
     7441 accacttcgc tttgcttttt gggtaatctt ttgccagtaa gcatatagaa tggaacacca
     7501 gaccatcgcc aattgtcaac ataggctttg atagcaacaa aagtttctgt tgtagatgcc
     7561 tgattagcac ccacctcttc caaataacca ggtatttttt taccatttgc taaagtattg
     7621 gccacatatt gggctcgtac tgtatgatca tgaacattgg acttgttaat aaaacgcaaa
     7681 gatttcagta cttttaattt ttcgcttctg atgctttctg ctgaaaggct taaggggggt
     7741 tccatcgcaa ctaaagataa tatttgtaac aaatgatttt gcagcatgtc cctgacttgg
     7801 cccgacttat cataatattc ccaacggcct tcaacaccga tttcttcggc aacagaaatt
     7861 tcaactcgat caataacgcg attatcccaa ttggttgaaa atatggaatt agcaaagcga
     7921 agcactaata aattcaagac ggtttcttta cccaggtaat gatcaattcg ataaatctga
     7981 tcttcagcaa aaaaacaagc cacttgttcg ttggttgcta cagaagaagg caaatcatgg
     8041 ccaataggtt tttccaaaac aactcgcgat ggtttctgtg ttagattaag gcgagcaaga
     8101 ctgcggcaag tttgagaata aaggcttgga ggaattgcca agtaagaaat aaccactcgc
     8161 tcagaagggt taacatagtc cgttaatttt tgataatcat ctgattgatt cagatcaagc
     8221 ttgcaataag ccagtttctt tataaaacgt aaccagactg cttcatcaat ttcatcaaat
     8281 aaaaattgct gaattttatt tttaatcaag tcaacataag cagaaaaatc aagctcttct
     8341 cgggcacacc caataattct tgttttttcg tgaagtaaat tagccagttc tagttgatat
     8401 aatgcgggca acaattttcg acaagacaag tcacccagcg taccaaacaa aattaaatca
     8461 caaggatact tcgattctgc ttttaagagc atatatcatc ccaaaattac aataactatg
     8521 tccctgaaat tgcatcgatt ttacatcact ggaatcaagc gtcaatcaaa attgattttt
     8581 ttcgctcatt ctatcaggta catcacctgt ttaatcattc aaatcaattg aagtgaatta
     8641 aaagcggtag aggataaaaa aggattaaaa aaagcacttg gaaatccgct ttatgaactg
     8701 caacattatc aaaattagcg atttttgtct aaaaattgca aaacttcatt tttatgctat
     8761 atgaaataag tacacgttag taaaaaatcc ctttgattag gagccactca tgcttaacaa
     8821 gagattttca gaacgactga ataaagaact ggataatatt ggagtccctg aggccacagc
     8881 agagcgaatt gagatacttt ccaaactcat caaaattcca aaatttaaag cagaagcttt
     8941 gcttaatgga attacaagcc ctgatgaagc cgtattaaaa acattagctc aggaactgga
     9001 agtcaatgcg gattggctaa ttggcaaaag tgactccaaa tcaggagccc attagaaatt
     9061 atccatgaaa tctatgattg gaactaattg gcttcgcttt tttctcaatg tattcaatga
     9121 tttttcctgc caaattaata ttagttgctt tttctatacc ttcaagacct ggagaagagt
     9181 taatttctag cactaatggg ccatgatttg agcgaatcag atccactccg gcaactctta
     9241 atcccattgt ttttgcggca ctaacagcaa ttgcccgttc ttgcggggaa agtttgactt
     9301 ttactgcttt tcctccttga tgaacatttg cgcgaaactc cccatccttg gcctgccgtt
     9361 taactgcggc aactactttt tctcctatga caaaacaacg tatatccgta cctcgggatt
     9421 cttcaatgaa ttcctggacc aaaatattgg catgcatttc tttaaaagca ttgataatac
     9481 tcacagctga ttgatgactg tcagcaagaa tgactccttt gccttgtgtt ccttctaata
     9541 attttatgac cagaggagcc ccaccaacca tatgaatcaa atcttcagta tcatcaggag
     9601 actgggcaaa actggttaac ggcataggaa ttcctttacg tgccagcaat tgcaaagagc
     9661 gaaatttatc cctggatcgt gatatagcta tagattcatt aagagtgtac atgcccattg
     9721 tttccatatg acgcaatact gccgtaccat aataggtaat ggatgcgcca attctaggaa
     9781 taacggcatc aaaatgcggt aaaggtgccc cacctcgata atgtacctta ggattagaag
     9841 cagccacgtt catataacaa tataaagggt taataatttt aacctcatgc cccgcagcct
     9901 cagcctccgc ctttaaacgc ttatgagaat acaaatgggg gttagttgcc aatatcgcaa
     9961 ttttcatgaa ttaaactttc ctcagtaatt tcatacttca atctgaacaa tccaaaccat
    10021 tttccctaat tttctattaa ataacgcaag ttttaagcat cacaacagag ctgctctgaa
    10081 atgacaaaag cagccgagcg atagaaaatc gcgggtttta agccaagata aatgaaatgt
    10141 caacaatcct tacttaaagc ctagattaaa aatcacaaat tatcctccct gccataccca
    10201 acaggagagt aatttttgaa ttcatgcaac agtcaattaa ttaactaatg catcctagat
    10261 agatttcttt gacataaata aaacaagtct gcttagctat tgctcctgta gaggtaaaat
    10321 atcgaattac aggttaagat accaataaga cataatcctt agctaagaga tccgcatgat
    10381 gtggtgttgt aaaaaatgca tttaactcac cttgaggatt aaatagcatt acggccccac
    10441 tatgttgtat atcataattt tcaggctccg aactctcttt actgtttatt tttgcgtaag
    10501 caatgcccat ttcatgggtc attgatttaa ttgaatcctc ttctcctctt gcaccataaa
    10561 atgatttatg gaaagaagtg acataactac ccaatttatc aagactgtct ctttggggat
    10621 caatagatat catcacaact tgaggtaaat ttttcacccc tttcttttcc agaattcgat
    10681 acatttttcc aagttctgcc atggtagtcg ggcatagata accacaattg gtaaatccaa
    10741 aaaatactaa agtccatcga ccttgcagac ttgagttatc aaaaactttc tgatctgtgc
    10801 ctgttaagct aaaattattg actgttcttg gattttcaag ataagtcccg tgaaaatccg
    10861 ccacattgat cttcttttta aaatgaaaat tctgagagat ataaatacca gccaataaac
    10921 tgaccaaggc caatacagtg attgttacag cgcccccttt tatctttatg ttcattctaa
    10981 ccccttaaaa atagtgatct actaataaaa agacaaacaa cagcattagg tacagaatag
    11041 aaaatcggaa tgtttgcata gcaacgactg gtttatcagt aaaatacaat ttatgtgccc
    11101 aaaccaaaaa cctgatgccc aaaaccaaag cacctagcaa ataaatccag ccgctcatac
    11161 ttacaacaaa aggcaaaaga ctgactacca gcaataaaac cgtatagaga taaatattta
    11221 atttagtgaa ttgaataccg tgtgtcacag gtaacatagg aatttcagca tcttgatatt
    11281 ctttatatcg ataaattgcc aaagcccaaa aatgaggagg tgtccatgtg aatataatca
    11341 atactaataa caaggcctga ggatccaatt ggtcagtaac cgcagtccac cccaataagg
    11401 gaggcgcagc tccggctagg cccccgataa caatattctg cgaagtagct cgttttaaat
    11461 accctgtgta aacaccagca taaccaatga gtgttacaaa ggtaagtaat gctgttaatt
    11521 ggttcacaaa caggataaga agacttaatc ccattagccc gataatgacc gcgaaccata
    11581 aggcttgctt tactgacacg cgcccataag cgaccggtct cttttttgtt ctggccataa
    11641 tcgaatcaat atgcctgtct accaaatggt tgattgcagc agcgctgcca gcgcacagtc
    11701 ctatgccaag taaagtaaaa gcgattaagc gcaagcttac ccagccagga gctgctaaat
    11761 acattcctac aatgacagtg agtagcatga gcaacacgac gcgtggctta cacaattcca
    11821 cgtaatcacg ccaatctaca ggaagtcgag ctgcgtattc agtacgcatg gctaacctcc
    11881 ctatcttgtg taaaatgcaa agaacaaaat gccaccgcta ataataatgc ggctacacca
    11941 ttatgagcga ccgcaacctt gagtggtaac agatgaatca cattcattat ccctaaagtg
    12001 aattgaagca atactaacat agccatgata atagcagttg ctcttaaata aattgaatgc
    12061 gttttgaata cgattattaa tgacagagcc agtagataag tggcagtcaa tatagcccct
    12121 attctatgaa taaattgaat gctggcccta acttcactct caagaacacc gccttggtaa
    12181 ttggcgccta tcgtagaaaa taaattaaat ccttttgtaa aatgcaattc aggaaaccag
    12241 actccgttac ataatggaaa accaatacac gaaatacctg cataattggc acttacccaa
    12301 ccacccaaag caatttgcaa taaaatgata acaattccca aacgcaacca aggtcgccat
    12361 tcaggtaaat tttgaccctt tagctgggct atctgcaaac gaaaacggca taaacaggct
    12421 acgattaaca tacctcccag caaatgtccc atgacaacta caggcagtaa ttttaaagta
    12481 acagtccaca ttcccaatgc cgcctgaaat accaccagca taaataaagc caagggaaga
    12541 tgccaaggca aatcttgatc ctgacaacgc ttgaaccata cctggaaagc aatgaaaaaa
    12601 atcaacaggg ctaacgttcc tgccgcatag cgatgagcca tttcggtcca ggctttcttg
    12661 gattcaatag gaaggtccgg atattgtgat tgagcttctt gcaaaccttt atctgctcct
    12721 ggaagaacca tgcggccata acaacctggc caatccgggc agcctaagcc cgcatcagtt
    12781 aaacgagtat aagctcctaa cataacgaca aaaaaagcca aaaaaacagc gagagtcgct
    12841 gctaatcgta ttgaactatt agacatcaat ttatccgctc ttaatttcag ttgtattgag
    12901 taaaagtttc aaatctttat atatatcacc tgggttacat cctggttgat agctcagtat
    12961 gaggtaatta tccgggtttg ctataaaaat tttagcttca ggatataaag tcgacaattt
    13021 agacatttca gttaatgata aggcagcaac atggatatct tgctctttta aaatgggttt
    13081 taattcctct gcgatcaatg aatctttgtt gcttagaagc aaccattcat ccacctgata
    13141 taattttctc ccaaaagaaa ggcggactct tcccaacata ttaagctgct taagacaagt
    13201 cttgtcacaa ccattaggat tccaatagat aatgcgccat tttgcactgc tatctaacgg
    13261 tttgagagcg atcggtggag aaagtaaaat tcctttattt actttagagg ttccaagcca
    13321 ggaaggatgt ttatagaaca aataagcggt tattcctggt gcagcaaaaa tcactaccaa
    13381 gagtaataat gtcatgtatt tatatggttt tttcattttt cttcaaatta aaaatcacga
    13441 atataatcag aataaccaat gccatggcaa accactgtat tgcataagca aagtgccttt
    13501 gcgggggcat ggatactatc tcccattcac gtacaaaacc aaaaagctca ttcttgtcga
    13561 ggcgaataat aaacggatag accttttttt gcaaaagttg tttcagtatt tcttcatcaa
    13621 tgagttcaag aagagtcacc ttattctctt tttcttcaaa acttggccct aatacccatt
    13681 gttttttact tggaaaataa accatgccaa atagtttaaa tttgccgttg ggagtctgaa
    13741 cattaggaaa agtgcgtcga gtaatgtctc cagaaaccca acctctatcc accataacga
    13801 tggagtcatc atcaagaagc ataggagaaa caacgtcata accaaattga tgttggtaat
    13861 gttgattgtc aagcaaaaat aactttggca gaaaagctcc ttcaatgctg attcgttgat
    13921 attgctctgg caatttttgc cctggctgcc aaattatggg ctcctgcttt gccagcgctt
    13981 gttgtgcagt tatcattgca gttttttcat cggctctgtg tatttgccaa aatccaaggc
    14041 taatgaataa aaaaaagcaa acggccgtaa gaataagcat tggccaattt gaggtgaaac
    14101 gaaaattaaa acaggttaaa ctaggcatct tttatcaact actcatttgg ttagctaaca
    14161 ttcccctgaa gctagcaatg tcaggagtat aacaaatgtt cactaaaatc ttcatcatct
    14221 ttgtcatgat tcttattgtg gcctcattag cgagcgggct cttttacctg gtcaaggaca
    14281 ctgataaatc aaagcgcaca gtcaaagcgc tcaccgtgcg cattgcaatt tcattatctt
    14341 tatttgtgtt tttatttatt gccttcaaat tgggattgat tcagccacat ggaatttagc
    14401 ttttggcttt taataaattc acctcaagag atagggtaat ggctgagata atgagatatt
    14461 tttaaatagt catttcagcc acctacagga aattccccct aatccacgag acgacaaaca
    14521 acgagaaaaa atctcccagc gagcatcaat tgaccgtaaa ccaataccac cgttccaggc
    14581 aggaggggct actctcttac aataaaattt cgaaattgct caatatacta tctatacttt
    14641 tgttgtagcc ctcaaaaggg atggtgatca tcatgatgat agttctgttt ttattttatt
    14701 tcataatagg attacaaatt atatttaaac ccaataaaac gataatactc caatttcttt
    14761 tcagtttgct tcttatacta atcagcttca attatcacag ccatctggtg aacctatgaa
    14821 taaacaaatg caatggcatc ttttcggcaa tacttcgggg ttattgataa tttgttgcct
    14881 tttgatcttg gccttcctgg atcaattcat cttttatgaa ctaccctgcc caatatgcat
    14941 tttgcaaaga atttgcttta ttgcagtagg tctctgtttt gttatgaatt taatgctggg
    15001 tattagagct tcacattacg ggctgatgct tttggcaact cttttagggc tgtctatctc
    15061 agttcggcaa atgtatattc atttaacacc aggtgatcct ggttatggcg gcctcatttt
    15121 tggtttgcat ctttatactt ggtcagcaat catattttta gtcattatct tattaattgc
    15181 tggggctcta atccttgata agggcttcat gcccgattat aaagtacgaa gcaagtcggc
    15241 aaaagtaatg atatgggtat tttttatctt gattcttgcc aatggaatca gtacattctt
    15301 ggaatgtgga ccgcacgaat gtccagataa cccgagcaaa tattatttac taaataataa
    15361 ttaaggatct tgaggattta aaatgcgtct tttgtttatc agtttaattc ttgccagcgg
    15421 atatttacag gccgatacaa taaataatta tatgaatatt gctaacaata ttccgcaaat
    15481 ggaaatgaaa gcagaccctc aagctcaagc atgggccaga tcggcgcgtc atgtacttac
    15541 catcaccagt gaaagtattg ctgaaacttt aatccaggcg aatgaagctg cgaaaacaca
    15601 agggaaacct atcttttgct taccacaagg tgtgcaactt aattcttttg taatgaacga
    15661 gctcattcaa caaacctata aagaaatatc cagtcaacaa agcgacaaag ataaaatgac
    15721 cgtttctcaa gtggcattat tagggcttag caaaaaatac ccttgtgaac ccagtcctca
    15781 ggagaaacaa attcaacatg tggcttccct gttatcgccg caatagcccc cacagcaaca
    15841 aagtcccgga attttttcta catccgtttc tttaagccag acgacatgtt ctgaaaattg
    15901 ttagggtgag catggcaaag caaagtgggc agagcgtcaa gcactgtgaa ttcccttgtc
    15961 aatgcttgca aaaactctgc cttgctcgtt ttttattgat tttagtattt attaagcgca
    16021 actttagcgt cttgataatt acttgctaaa ttcctcaaaa gcttccaatg cttctgcagc
    16081 atacattaga gaagggccgc ctcccatata aaccgccata cccaatgttt ccagtaactc
    16141 ctctcttgtc gcttgcagtt tcactaaagt ttgtgaatga aaccctatgc atccggggca
    16201 ttgcttagcc acggccaacg ccatagcgat taattccttg gtttttttat ccagtgcacc
    16261 atctttcgtc gcagcttgtg ccaaagaaga aaatccagtc attaactctg gcatctcttt
    16321 gcgaaacttg gctaattgcg tagtgatatc ttttgtaatg tgtgaaaatt tgtctgacat
    16381 gaggctctac tcctttcctt attatatttt ttcaagcgtc tctaatttac agcataatat
    16441 tgggatgcaa aagatatatt ccctaattta acgtgagtaa tgtataagac aggtcgttat
    16501 cttgaatcat ccatgctgag gatatcgcct gattcttata caatccctat cgataacgtt
    16561 aatttaagaa gtagcgattt caggatattg ctcgattgag cgtttcctta gccagagtaa
    16621 gtcttttttc aacattccct aattcggttt gtaaatgcaa aatagttgca ggattagagc
    16681 aggacttcat ttgctctaaa gtttttctca tggcttgata ttgttcctcc agggcacaga
    16741 tcttctcttc catggtttta atccattgtt gtcttgtact aagagttgtc aaatcaaaag
    16801 aaatttttga ttcaggttta ttattctttt tgtccatttg gcataatacg accaggacat
    16861 taattttttt ctgaatacgc tctgccaaaa aacatgcaat tgactcatcc ttatgtttct
    16921 gtaaagcgtg aatatcatcc tggatttcct tgatacaggc aggtccatta aattctataa
    16981 cggaagaaaa tatgcctttg ggcaaattat gaattgaaat agaagaactc aatccattga
    17041 tcttccattc cagctctggc aatcgtgcag atagttcttc caacaattcg tcaatatagc
    17101 ccatcttttg aaagttacat aaacaactgg ggttattaaa cttaacctgt cttgttaaat
    17161 tatacagcag gttccaaatc cgcctctttg gcttgcaacc aactggctat aaaaggagga
    17221 gcacacatga gcagcaagcc aataatcaag gtcaattcat agcgtgttgc actgccgaca
    17281 ttaatcccct ctggaggaat aaagctaacc gctaaagtgg taaccactcc aatgatgcct
    17341 atgcctgcaa caaacaacat tcctgccata cctcctggaa ttttaaaagg tctgggatgg
    17401 taaggttggc ttatgcgcaa tttaattgct gcgacaaaca tgatcaaata catcagcatg
    17461 tataattgag ccgccaatgc tgttaagagc caataggatc cattgacact gggcataaat
    17521 aagaatagcg ccgataatac tgtgactatg gttgcctggg tgtatagcat catggccgga
    17581 gcaccttttc cattagtccg ttgaaatacc tctggtaaat tcccttcttc tgcggcaact
    17641 aataaacctt tggtaggggc aataatccaa ttactcacac caccaagccc tccgagaacc
    17701 aacatcacag ctaccacagg catcatccaa tccatgtgat atttaaggaa aaaggcagaa
    17761 aaggcttgca taattccggc aaccagattg atatcctttt gtgggagtac taccgcaatc
    17821 gctaatgaac ctaaaatcaa agtactcaga atgatcccta cagaataaac cagggctttg
    17881 gggaaagcat gctggggatc cttcacatca ttagcatgaa ccgtagcaat ctctatcccg
    17941 caaaacgaca atatgatggc ggttaatgaa acccacatgc ccttatccga taaatgggga
    18001 acaatactgg gcatgtcaaa actgatttgc aaggggttac cttgagctat ccaaacagca
    18061 cccaaaccaa tgatcaaaga catgggtaat aataatcctg ccaaagaaca aatattactg
    18121 aacatggccg atgatttcat accgcgcaaa ttcacaatgg ttgctcccca aaagcaactg
    18181 acaatgactg cccaaagaaa ataaggattg gtagttaaag aggggttaat aagataacct
    18241 attgttcccg caacaaacga tagaatcgtg ggataccaaa tcacattttc tatccattgc
    18301 aaccaaatgg ctaaaaaccc tgattttttc ccaaaagctt ccttaaccca gatataaacg
    18361 ccaccctgcc tggcccaccc agaagctaac tctgccgaga ccaatgcggt tgggataagg
    18421 aagaacaagg cacccaggat aaaataaaaa atcaattgac tcccaaataa tgcagtagct
    18481 ggcaagttac ggatactgtc tacagagcca acggtaatca tagtcaagct aaatatagtg
    18541 agagaatgtt ttttattgct cattgtattc cttcagtgag gactatttgg aatcaaaag
//
LOCUS       DACTJE010000052        18144 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.052, whole genome shotgun sequence.
ACCESSION   DACTJE010000052 DACTJE010000000
VERSION     DACTJE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18144
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.052"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(233..397)
                     /locus_tag="I9760_001389"
     CDS             complement(233..397)
                     /locus_tag="I9760_001389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043162.1"
                     /translation="MLAATVEIFCDIDDFCKAWFKEMSPYLLPLPNRKRQRPCRLSAS
                     EIKIILSLTE"
     gene            complement(494..781)
                     /locus_tag="I9760_001390"
     CDS             complement(494..781)
                     /locus_tag="I9760_001390"
                     /inference="COORDINATES: protein motif:HMM:NF024412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT5043163.1"
                     /translation="MDAGYVCRKCGISRPNLRKWYRRYLKAGIDGLNDESKKPHLFPG
                     KLNSELIKLTLDLRISRNLGARGIQAELVRLPKCPLSLASIHKSLTNLTSI"
     gene            complement(992..1282)
                     /locus_tag="I9760_001391"
     CDS             complement(992..1282)
                     /locus_tag="I9760_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAT5043164.1"
                     /translation="MRSFYVYIMASKRNGTLYVGSTVDLIKRVWQHKQKVVPAFTATY
                     NVHILVYYEIHQSYIEAARREKRFKNWPRQWKINTIEQLNSEWRDLYEDICR"
     gene            complement(1337..2086)
                     /locus_tag="I9760_001392"
     CDS             complement(1337..2086)
                     /locus_tag="I9760_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011789342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HAT5043165.1"
                     /translation="MPRLMLSDELWSKLKGIMLQHKIYDKPNLRMIVEGMLYHMRVGC
                     PWRDLPAAFGCWNSIYQQFNRWSFKDKLMQIFKALVHAPDLEWEFIDGSIVRAHQHST
                     GACSTESEAIGKSRGGNTTKIHMAVDAFGLPIEFQLTGGKVHDAKAAPELIEQLPKSD
                     YVIADKGYDSEDIREQIREKGSIPNIPRKSNSKTGNKDIDWYLYKYRHLVENMFACLK
                     HFRAIATRYDKLKRNYASMIAIACSCMWLPM"
     gene            complement(2267..3289)
                     /gene="ravT"
                     /locus_tag="I9760_001393"
     CDS             complement(2267..3289)
                     /gene="ravT"
                     /locus_tag="I9760_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavT"
                     /protein_id="HAT5043166.1"
                     /translation="MHECVIQIAILGYKRMYSKLLRGAATAVAGYVSYVLLNETKREH
                     EIKSKEEIKDCEDLVKNSSEFKEIGRPIHGTLSSSYVQHIETGNIYVKKGAHSRRDLV
                     KELMVSNALHKIREDQPECLIMQTPIKNGFQYHTLSRKFDHTQDVEEFVRNGRIDELR
                     KKEVIGLGDTLITDHIFGKQSDTKLANMVVRDKQDKLIFTSIDHERAVNPTSFSFFHP
                     TPPIYTKNKSTLIRSINDLGEQSEDNHAGLAGDPRAKEFGEVVENVINNLDIDNYYQK
                     VATTNLSSVISQCEKLAKASKGRLVQSEDCAGYESFFKEMQRNAKNQLESSKPQDDTP
                     VLRKSY"
     gene            complement(3381..4268)
                     /gene="ravW"
                     /locus_tag="I9760_001394"
     CDS             complement(3381..4268)
                     /gene="ravW"
                     /locus_tag="I9760_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavW"
                     /protein_id="HAT5043167.1"
                     /translation="MKQKIDYIARYFKLMSPIINREEINNIVKAQDELEITGAPEHGS
                     HKLSIVKELETGFEYVQKQTKNQTETEKEFMMASFLNKVNPNHPKCKLVETNNGSVSI
                     LSRKQENTQDVEQFVRAGRTNELLEKKVIGLEDTLIADNILGKQSDTKLANMLVKDEG
                     DTLVFSNIDHERANLPTFSLFNSGQRRYPTSAHELVSGIADLYEPSDDNRSGLAGDKR
                     AKEFGEVATKVIKQEKIKSAYEKVANADIDSVYKKCSSLSQNSTFFGGKNNCNAYRQY
                     FKEIQKEAADTVSKFDLKK"
     gene            4818..6800
                     /locus_tag="I9760_001395"
     CDS             4818..6800
                     /locus_tag="I9760_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043168.1"
                     /translation="MNKILLFVFGSIGLSLNIEISAAKGIVCKDYDGNPMTVKPKTIT
                     IFNNSEVTIYPVLATSKNAVNEWIQGCFRTTESYPTNYVYKLYVNEGTGIVPGSSVKI
                     TLPLYSQLANDRYITWWNGGRIVLADKNERLRNENDQALSTPEDVSCDGTNTECKLSV
                     YSSDVQFPENIYAQLSEYTFGDSIIPPKQSTRILKPENVGYNISYVDHVYMPVAIGPK
                     NNPYIGYSGSVMSLPTFRSHLNSFLETNLGHGWPVYNLNELKLPGGYNVFAQRSGTLP
                     PGDNVPVKPNDGFPPVLTVLACIQGDCTEEQKASLHFGESVQRMQNLWGSCVNWDEED
                     ISKYITQKVDCPQDLKGKLSAVQQFFKKNHQQYLQLYEHGKCNLTPGIDPVPFNYWEA
                     IKHIYGWVPFNEGCGAGANPLAETNIPGWDHAGIQSMYIHDLQYNYQNKPIPELLFNP
                     YVQLIHDKNYLSMDAYGFSVDDAVGFMSELGDGLIFTVGGTKGLENQQEFNYADGFSV
                     AIGVPLSLLDQVNTPMIKKYGVCVFNQDANDLNCQEVKQNVIMPTNSQIAGFRVGTVA
                     SYPIKVFFTDLNDNLYTFVVNTKFAPCSAEMDPSQCPTNKAEIIDTKSCIVTKSNGEK
                     HPKSNNWCQNANPNQQREKQLTKNFISFPPPVDYME"
     gene            complement(6933..8417)
                     /gene="gspE"
                     /locus_tag="I9760_001396"
                     /gene_synonym="lspE"
     CDS             complement(6933..8417)
                     /gene="gspE"
                     /locus_tag="I9760_001396"
                     /gene_synonym="lspE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE family T2SS ATPase variant LspE"
                     /protein_id="HAT5043169.1"
                     /translation="MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPNTPLQAFA
                     EIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILDAAEGMEEDMDLSMLASQLPVSE
                     DLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
                     IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILD
                     KQAAQLDLNLLGMPEHTLKTVRQMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTR
                     NILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVVMIGEIRDLETAEIA
                     VQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKT
                     PHELRQEEKELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIH
                     RNENIQTMENYLRPTTPSIREDGFKRVLCGDTSLAEILRVTSQN"
     gene            complement(8421..10796)
                     /gene="gspD"
                     /locus_tag="I9760_001397"
                     /gene_synonym="lspD"
     CDS             complement(8421..10796)
                     /gene="gspD"
                     /locus_tag="I9760_001397"
                     /gene_synonym="lspD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspD family T2SS secretin variant LspD"
                     /protein_id="HAT5043170.1"
                     /translation="MRSIVIILLAFVCCSLVRANDVNISTVTKDGNTFFYLQAGAYNL
                     EKDAKIRQNELAKLVDQKVEIKNLADKHLYLVQIGPIDDYQTARALKEKLSQKTEKQF
                     NKISKNQTSSLEVSQLPKNMEQAQTAPPGSKLWNLRNADIRAVIAEVSRITGKNFVID
                     PRVQGKVSIVSSTPLSSRELYQVFLSVLQVSGYAAIPNGEIIKIIPNIDAKTQSPDLL
                     SGMKSPPRGDDMMVAVIPVHYVPSEQLVPVLRPLMPQWSSVSAYAPSNMLILSGRANN
                     IKSLAEIIKQVDSSSANGIDMVRLHHALAMDVANTLKDLVKTQPGIGSRTQITIAADD
                     RSNSILVSGSKTDRIRLRMLILKLDKDSSTGVNSNTQVVYLNYLRAEDLVPILAGIAQ
                     ANFSGNVGTTIGTITRPALDSTNPASSLANTSADGQSSSLSSTSTSSAPMNASGATAN
                     TTSASTQNEGSTKPTVQIIGEPNTNSIILNAPASIIRILKTVISQLDIKPAQLLIEAL
                     VAEVDEKDVNNLGIEWGSNQQTGNPKDFRPGFAIINSKTRIDDFQAQIYALAREQRAN
                     ILSTPSVVVLDNRQAKILIGKQVSIATTSYPNNAGGTTTASPFTTFDRVNVALHLYVR
                     PQITRGQGIQLQIDQGNDTLDPATATTDNTTTPTFNISSIVTSVHVESGDIVVLGGLI
                     QDSIGNDNNKLPILGDIPGIGRLFQRNIRNRDKRVLMVFIKPIILRNERDNLHVTGEK
                     YNYVRQYQLDWIRSQEAFEQTDDETVLPPLTQGNLPVPFSTPPQYSSKMTK"
     gene            10969..11997
                     /locus_tag="I9760_001398"
     CDS             10969..11997
                     /locus_tag="I9760_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAT5043171.1"
                     /translation="MDNAIIKWGILGTSFISEVMANAIQESKTSELVAIGSRTSKIAK
                     RFSEKFAIPKFYDNYQSLILDSDIDVVYIGLPNHLHKEWIIRCAQAGKNILCEKPFVV
                     GIEEIHEVISVIERTNVFCMEALMYRYHPFIKKLQEIIQSNTIGKIKLYNATYTANIA
                     EIANPVAGGSIRNLGCYPISLVRLLANAEPIEIRGIGRMNSRNNTDSQASVLLKFEDD
                     SIAAVSTADDIEMHWQFEVYGTKGHLKVETNPWLPGSEGNKIYISLNTQSTPREISVK
                     AEKSLYTYQIDFINEQVLKGDSRQFSKTSLLDSMGNTIVLETWLKQILSSKPSLELSR
                     LKDRLPHA"
     gene            complement(12018..14123)
                     /locus_tag="I9760_001399"
     CDS             complement(12018..14123)
                     /locus_tag="I9760_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HAT5043172.1"
                     /translation="MDSNFKISIRVSIITLFVLLLSLVGFTIIGINYIAFNSVLNSSA
                     KDSIEQTSLLVKERFEIYLNPLNQDLIKITNAINNGIINPDDSKQFDKFLFESIRYNP
                     EIFMVYYGSTTGDFIGVDREQKGIIGLTHVINSVSPPVNVRYQLNEQGEIIGKKLLTR
                     HYDPRVRPWYQQAIKAGKPIWTNVYTFYVFDKSDFLIPGITGASPIYNKNKQIKGVIA
                     LDLTIDGLQHFIRELELTKNTMIYVINNESNIIAFRTPQNKKDIRGKKLTPEWIKKLN
                     IPLKKLDFNDFEPIIKSYTHDNQKYYLAYQPISSTNEKALWHIIIIVPETDVIEPLKD
                     LSMRYILLTILVLLIGTLLVRIVSQRISNPIIQLTEEAKEITMLNLKPRPLLKTRIKE
                     VSYMDKTLSSLRSSLASFQRYVPGSLVKKLMRSGKIAQVGGQSQTITILFSDIKDFTS
                     ISESTSPQKLMTYLSDYFQSMTEIVIQHEGTLDKYIGDAIMALWNAPSKDTNHSLHAC
                     LAAKIMIDRLSLLNQKNKHLGFPEFKIRIGIHTGDAVVGNVGSEDRLSYTALGDSVNL
                     ASRLESINKNYHTQIIVSHATYTQVSEKFPFRLLDEVAVRGKRESIEIYELITAQNLE
                     NLERHKKEFQKAFSLYQKGSWKESISAFAKLTPAYSDDQLASVYIERCKVLAVNPPVN
                     WDGIWREGKTDYSLLGKFD"
     gene            complement(14267..15427)
                     /gene="lbtC"
                     /locus_tag="I9760_001400"
     CDS             complement(14267..15427)
                     /gene="lbtC"
                     /locus_tag="I9760_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038838172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="legiobactin import MFS transporter LbtC"
                     /protein_id="HAT5043173.1"
                     /translation="MKKNALLIVLLTWFLGNMDIHFLTPALPSLVDYFSVTPNTVQLT
                     ISLFLLGKALSMILWGLLSERYGRKPIFIGGLLLFILSNFLIALNHSILPFLVCRFLQ
                     GISVGATLLMGRAMINDTHNEQNATKYFAWLFTLAGLFICFLPFFGGLINSYWNWQIA
                     SLIIAAYGLLLLPLCRGLKETKPHYVKFPRLGHSIMVVFNHPLFVSYLLISALMMAGE
                     SAFNTSASFILIKGENFTSAQYGTIKTTMAIMHLLGTACCGLIAKYYHSIQLTKLGVR
                     FFIFAAFLMWLFVFSAENIYLTFIVPMVIYYFGTGFIVASATASAVRPFPQQMALALA
                     ITLFCQFNCSAFFSFITSMIGIQQVETFMFLLSLVSILSFLALLKLQRMEPC"
     gene            complement(15424..16638)
                     /gene="lbtB"
                     /locus_tag="I9760_001401"
     CDS             complement(15424..16638)
                     /gene="lbtB"
                     /locus_tag="I9760_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="legiobactin export MFS transporter LbtB"
                     /protein_id="HAT5043174.1"
                     /translation="MNKLVQKMMLSGQFLMILALEMTNPFLPLLIAHQSNTPVPSVVI
                     YNTLSLVLPMLANILLTPIWGLAADRYGYKPMLMRASWALVLTQASMIFVNSVFWILI
                     IRLLQGAFAGFLVAMQTYALSITEWQSKSTQLSRLQSSKAIATSTAGFLGGLALSFTN
                     YQGLFGLATLICLGTTVVMHYKLPSPPKQQIKKRSQTLNHSYTSKSVFFFLCVLIMLT
                     QIAKFLPDPGFTLYLNKYCSNNLVLIGFLYSLPAMGMLCSSVWCGKQFDYCRSQPSLV
                     NQYLIRYSVFGAILMIIQANVDNFYLFALIRILWGIVLAALLPALFALCSDRNLLPGY
                     ALGLANSFAKLGNLIGLLLGGLFAFYLPYPAIFMIIAGIYSLFAIFVYGYDRLNVRQM
                     TDFSNSYFNVKS"
     gene            complement(16631..>18144)
                     /gene="lbtA"
                     /locus_tag="I9760_001402"
     CDS             complement(16631..>18144)
                     /gene="lbtA"
                     /locus_tag="I9760_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="siderophore legiobactin biosynthesis protein
                     LbtA"
                     /protein_id="HAT5043175.1"
                     /translation="KQKPEKTFDRWPLLKKELDESINNQALALAYYHCWQQSLKQQAK
                     NHSSLWSWLSEQHNKQQILTFLEQWGCIGHPYHPNFRAKMGFIPEEVIRYSPEFNAQV
                     DIPWLAIHRSVAFTSIDATSYSLLFAQHFAKEYQLWNYSVQKQQLDPDQYLPLPVHPW
                     QWNNKLQTLASVLINSKQLLITEAVQKTKPSMSFRTMMPLDNKGPHLKLATAIHTTSA
                     LRTVSPASVSNSSPLSSWLRQLLTQYQSFEKPLFLARDLAGINTTHPSISPQNKKHLA
                     MIVRENPLELIDASQTLVPVAALYAISPVSQRALFVEIIEASGMEPELYFIEYSRCIL
                     ECQLHLLLSYGVALEAQQQNTLVIFQSHRPVGLVIRDLGGIRVCFNSLYETTPKPNLH
                     PDSTISCHELSDLFNTFIYGNLLSNLTFCINCLHDNYNLTKKNLWILLKQIANQLLQN
                     FKKEINPLIYQKFQQHLFSLPWQHKSLLSMRLNQNQDNLVFFSLDNPLSQDNE"
BASE COUNT         5507 a         3289 c         3507 g         5841 t
ORIGIN      
        1 gatgaatatg tgcccctact tacaaagaaa ggattctttg cggtacaaat tgaaaaggtg
       61 ccagaaaaaa atgttaatca aaataataaa caaaaatctc ttgaggaagg agagccgcct
      121 tgctgcacaa taatttaacc tcagctcgac atatttttat aatgtgtagt agttcaaact
      181 tgatctgaca gttaccggtt tttcaagtag ttgtgtcaga ttaagttgaa actcattcag
      241 ttaatgacaa aattatctta atttcactag ctgacagcct acaagggcgt tgtcttttgc
      301 gattcggtaa tggaagtaaa taaggcgaca tttctttaaa ccatgccttg caaaaatcat
      361 caatatcaca gaatatttct actgtcgctg ctaacatctt gtttcctttc ataattcaga
      421 ggtgagtttt aaagaaacaa aatgtatata gtaccgataa ttttttcaat aattcaatga
      481 catagactta ttcttatatc gaggtgaggt tagttaatga tttatggatg ctcgctaaag
      541 aaagtggaca tttgggcaat ctaaccaatt cagcttggat acccctggcg cccaaattac
      601 gactgatacg caaatcaaga gttagtttaa tcagctctga atttaacttc cctggaaata
      661 aatgaggctt tttgctttca tcatttaatc catcgatacc agcctttaga tagcgtctgt
      721 accactttct caggttagga cgagaaatac cacatttacg gcaaacatat cctgcatcta
      781 tagtctcaag atatagattt acctaagtta acctagcttt aatttgctgt ttcataaggt
      841 gggtaaatct atcaattaat tgaaacgatg tctatgaatc acacaattaa acatattttg
      901 tataaggtca ctggtaatta ctagggtctg ttgacaattc actcctccag cctacgtgac
      961 gcggcttgtc cgcggcatcc agacgatctg attagcggca aatatcttcg tatagatcac
     1021 gccattctga atttaactgt tcaatggtgt ttattttcca ttgtcgaggc cagtttttga
     1081 aacgtttttc acgccgtgca gcctcgatgt agctctgatg gatttcgtaa taaactagaa
     1141 tatgcacatt gtaagtagca gtaaacgccg gaaccacttt ctgcttgtgc tgccaaacgc
     1201 gtttaatcaa atcaaccgtt gaaccgacgt ataatgtccc attacgcttg ctcgccatga
     1261 tatagacgta aaatgacctc atcctgcttc cttgttgata ttggactcac aggaatgata
     1321 acactggatg gtgtgattac ataggcagcc acatacaaga gcaagcgatt gcaatcatgg
     1381 aggcataatt tcgtttcaat ttgtcatatc gcgtggcaat ggcgcgaaaa tgtttaagac
     1441 atgcaaacat gttttccacc agatgccgat atttgtacaa ataccaatca atatccttat
     1501 taccggtttt ggagttactt ttccgtggga tgtttggtat agaacccttt tccctgattt
     1561 gttcccgaat gtcttcacta tcatatcctt tgtcagcaat aacataatca gatttgggca
     1621 attgttcaat tagctctggc gcggcttttg catcatgtac cttgcccccg gttagttgaa
     1681 attcaatagg taacccaaag gcatcaacag ccatatgaat ttttgttgtg ttacctcctc
     1741 tggacttacc aatggcttcg ctttctgtac tacacgcgcc tgtgctgtgt tggtgagcac
     1801 gaacaatact cccgtcgatg aattcccatt caagatcggg agcgtgcaca agtgctttaa
     1861 aaatttgcat taatttatct ttgaatgacc aacgattaaa ttgttgataa attgaattcc
     1921 agcatccaaa tgctgctggt aggtctcgcc aagggcaacc aacacgcatg tgatataaca
     1981 ttccctcaac gatcattcgc agatttggct tgtcataaat cttgtgctgt agcatgattc
     2041 ccttcagctt tgaccatagc tcatcactaa gcatcagtct tggcattgca aactcatttt
     2101 atatctgggg tgagaaccag tatattatga gtttgcttct acttatcaat agattaactg
     2161 attattcaaa ctcggtgtta cctttaccat cgcctggatg ccgcggacaa gccgcggcac
     2221 gtagggtgga ggagtgaatt gtcaacaggc cctagcaact agttggctaa taactttttc
     2281 gcaggaccgg tgtatcatct tgtggtttag atgattctaa ttgatttttt gcatttcttt
     2341 gcatttcttt gaagaaagat tcataaccag cgcaatcttc tgactgcact aacctgcctt
     2401 tactggcttt agctaacttc tcgcattggc ttattacact agacaaattc gttgtagcta
     2461 ccttttgata ataattatca atatccaagt tatttataac gttttctact acttcaccaa
     2521 actcctttgc tctgggatca cctgctaaac ccgcatgatt atcctcgctt tgttcgccta
     2581 aatcatttat ggatcggata agtgtagatt tattctttgt ataaatagga ggggtaggat
     2641 gaaagaaact aaagcttgtt gggttaactg ccctttcgtg atcaattgaa gtaaaaatta
     2701 gtttatcttg cttatctctg accaccatat tagcaagttt tgtatcagat tgtttaccaa
     2761 aaatatgatc agttatcagt gtatctccta atccaatgac ttcttttttc cttaactcgt
     2821 ctattcgtcc attacgaaca aattcctcaa catcttgagt atgatcaaat tttcgagaca
     2881 aggtgtggta ttggaagcca ttttttattg gtgtttgcat aattaaacac tcaggttgat
     2941 cttctcgaat tttatgaagg gcatttgata ccattagttc ttttactaaa tccctacgag
     3001 aatgagctcc ttttttaaca tagatattcc cagtctctat atgctggaca taagatgatg
     3061 agagagttcc atgaattggc ctacctattt ctttaaattc ggatgaattt ttaacaagat
     3121 cctcgcagtc tttaatttct tctttagatt ttatttcgtg ttctcttttt gtttcattca
     3181 gcaacacgta agatacgtat ccagccacag cggtagcggc gcctcgtaat aatttactat
     3241 acattcgttt atatcctaaa attgcaatct gtatcacgca ttcgtgcatg actggggcct
     3301 tttggaatag ccatagtaag ctagagagcc taagactaca accatctagc ttattatatt
     3361 gatcagggta gatttactat ttattttttc aaatcaaatt ttgacacagt gtctgccgcc
     3421 tctttctgga tttccttgaa atattgccga taggcgttac aattattttt acctccaaaa
     3481 aacgtactgt tttgagataa agagctgcat tttttataca ccgaatctat atcggcattt
     3541 gcaacttttt cataagctga tttaattttt tcttgtttta ttactttcgt ggccacctct
     3601 ccaaattctt ttgctcgttt atcaccagca agacccgaac ggttatcatc actaggctcg
     3661 tatagatcgg caataccgct aactaattca tgagcagaag ttggatatct cctttgtccc
     3721 gaattaaata aactaaacgt gggtaaatta gctcgctcat gatcaatatt agaaaaaact
     3781 aaggtatcac cttcatcttt cacaagcata ttcgccagtt tagtatctga ctgtttgcct
     3841 aaaatattat ccgctattaa cgtatcttca agaccaatca cctttttctc taataattca
     3901 tttgttcttc ctgctcgaac aaattgctca acatcttgtg tgttttcttg tttgcgagat
     3961 aagatactga ctgaaccatt attagtttct actagtttac attttggatg atttggattg
     4021 accttattta gaaaacttgc catcataaat tctttttcag tttcggtttg attttttgtc
     4081 tgtttttgaa cgtactcaaa tcctgtttct agctctttaa ctatagaaag tttatgtgat
     4141 ccatgttctg gtgctccagt aatttcaagt tcatcttgag ctttaacaat attattaatt
     4201 tcttctctat taataatagg agacatcagt ttaaaatatc gtgcaatgta atcaattttt
     4261 tgtttcataa gaacacctca aattttatat tttatattta accaatgatc agttggctta
     4321 ataaataaac attttaaatt aaaagaatta aggctttatt tggaagttgt cgtatttgtg
     4381 ccaaagacgt tagacgaagt tattttgggc ttatttaagc cgctaatgca tagaattgtt
     4441 cagaacgaat cattcttttt gataaaatat gttggttttt tctcaacata tgatgcagtg
     4501 caagaagcgg tttcccacta ttctgatcga gtaagtcagt ctttgacagc catttccata
     4561 taaagcggag cttgttgtct tatcagtttt ttagacaaga tataatcttg aaatgagcac
     4621 atatttttca atttcacttc gaatagcctc agagaggctt gagccgtgtg cggggaaact
     4681 cgcatgcacg gttcttagga gagtggtggc cggtaacggc cactgcttat ccgaccaact
     4741 cgctgacaga tgattgctaa tttgaaaaat tactttttgt gctattttta taaaatattg
     4801 tacaaagaga aattaatatg aacaaaatac tgctttttgt ctttggaagt ataggtttgt
     4861 cattgaatat agagatttct gctgctaaag gcattgtgtg caaagactac gatggcaatc
     4921 caatgacagt caaaccaaag acaatcacta tatttaacaa ctctgaagtt acgatttatc
     4981 ctgttttggc aaccagcaaa aatgcagtaa atgaatggat tcaaggctgt tttcgtacca
     5041 cagaatctta tccaacaaac tatgtttata aactgtatgt gaacgagggg acaggaatag
     5101 ttccaggttc ctcagtaaaa attaccttgc cattatatag tcaattagca aatgatcgtt
     5161 acatcacttg gtggaatgga gggcgtatcg ttttagctga taaaaacgaa agattgagaa
     5221 atgaaaacga tcaggcatta tcgacccctg aagacgttag ctgtgatggg acaaataccg
     5281 agtgtaaatt aagcgtatat tctagcgacg ttcagtttcc agaaaatata tacgcgcagt
     5341 tatcagaata tactttcggc gactctatta ttccacccaa gcagtcaaca agaatattaa
     5401 agccagaaaa tgtaggctat aacatttcct acgtagacca tgtttatatg cctgttgcaa
     5461 tagggcctaa aaataatcct tatattggat atagcggttc agtaatgtcc ttgcctacat
     5521 ttagaagcca tcttaattcg tttctagaga cgaatttagg acatggttgg cctgtttata
     5581 atctcaatga attaaaatta ccaggaggtt ataatgtttt tgcacaaaga tctggcacat
     5641 tacctcctgg ggacaatgta cctgtcaagc caaatgatgg ttttccaccg gtattaacag
     5701 tgttagcttg tattcaaggg gattgtaccg aggaacaaaa ggcatcactc cattttggtg
     5761 agtcagttca acgtatgcag aatttatggg gttcttgtgt caattgggat gaagaagaca
     5821 tcagcaaata tataacccaa aaagtggact gtccacaaga cttaaaagga aagcttagtg
     5881 ctgtacagca attttttaaa aaaaaccatc aacaatattt gcagctgtat gaacatggga
     5941 agtgcaattt aacccccggc atagatcctg tgccatttaa ttactgggaa gcgattaagc
     6001 acatatatgg ttgggttcct tttaacgaag ggtgtggtgc tggcgctaat ccattggcag
     6061 agacaaatat cccaggatgg gatcatgctg ggattcaatc catgtacatt catgatttac
     6121 agtataacta ccaaaataaa ccaatacctg agttattatt caatccttat gtccaattaa
     6181 tccatgataa aaattatctt tccatggatg cttatgggtt ttctgtcgat gatgctgttg
     6241 gctttatgag tgagctagga gatggattga tttttactgt tggtgggact aaaggtcttg
     6301 aaaatcaaca agagtttaat tatgccgatg gtttttctgt ggccattggt gtgccattat
     6361 ctttattaga ccaagtaaat actcctatga ttaaaaagta tggtgtatgt gtgttcaatc
     6421 aagatgccaa tgacttgaat tgccaggaag taaagcaaaa cgttattatg cctactaata
     6481 gccaaatagc gggatttaga gtaggtacgg tagcttcata tcctatcaag gtatttttca
     6541 ctgatttgaa tgataacctg tatacctttg tcgtcaatac gaagtttgcc ccatgctctg
     6601 cagaaatgga tccatctcaa tgtccaacga ataaggcaga aattatcgat acaaaatctt
     6661 gtattgttac caagagcaat ggagaaaagc atcccaagtc gaataattgg tgtcaaaatg
     6721 ctaatccaaa ccaacaaaga gaaaagcaat tgactaagaa tttcatcagt ttcccaccgc
     6781 cggtagatta tatggagtag gtgctgaatt ataatgcgac tttagaactt tttcttaggc
     6841 taattacttt gtttaattaa gctgataaat taaatgatta atatcttgaa gatgtggtgt
     6901 gactgtttaa aaattgctaa agtatgggta atttagtttt gagaggttac tcttaaaatt
     6961 tctgcaagtg acgtatcgcc acataatact cttttaaaac catcttcacg gatgcttgga
     7021 gtggtgggtc gtaaataatt ttccatcgtt tgaatatttt cattacgatg aatcattcct
     7081 ctcagggttt catctacgat aattaattca taaattcctg ttcttccctt ataacccaag
     7141 tggttgcaat agtcgcatcc cttgggttca aagacctgag atacatcagc atcaggttta
     7201 aggcccatta actccttttc ttcctgtctt aattcatggg gtgttttgca atgagagcat
     7261 aatttcctta ccaaacgttg tgcaatgagg ccgactatac tggatgacaa taaaaaagac
     7321 tcaactccca tatcatgcag gcgagttaat gctcccaatg cggtattggt atgaagggtt
     7381 gatagtacca aatgccctgt taagctggct tgaacagcaa tttcagccgt ttccaaatca
     7441 cggatttctc ctatcatgac aacatccgga tcttgacgta atatagccct taatcctttg
     7501 gcaaaagtca tttgaacttt agtattaacc tgcgtttgtc ctacaccagg caggtcatat
     7561 tcaatgggat cttcaatggt taaaatatta cgagtcacct gatttagttc ggtgagcata
     7621 gcatagagag atgttgtctt gcctgaacct gttgggccag tgactaaaat aatgccatga
     7681 ggttcagcaa tcatttgtcg tactgttttt aatgtatgtt caggcatacc caaaagattt
     7741 aaatccagtt gagcagcttg tttatccaat atccttaaaa ccactcgctc tccatggttt
     7801 gagggtaaag ttgagaccct gacatcaata ttgtgtcctc ctatacgcaa tgaaattcga
     7861 ccatcttgag gaattctctt ttcggcgata tccaatttgg ccatcacctt aactctggaa
     7921 atgaccaaag gagcgatagc tcgttgaatt tcaaggacct cttgcagaac accatcaatt
     7981 cgattgcgca ccaatactct gtcttcataa gtttcaatgt gtatatcaga tgctttttgt
     8041 ttaatcgctt gggtaaacag agcgtttagt aagcggataa ttggggcatc gtcttggttt
     8101 tccaataagt cttcactgac tggaagttga ctggccaaca tggataaatc catatcttct
     8161 tccatgcctt ctgcggcatc cagtattgaa gatttagatt ggtaaatatt ggctaaatgt
     8221 tgctgaaaag tggattcatc gacttgtttt agatcaagtt cgcattgcaa taatcttttt
     8281 atctcagcga aagcctgcag tggtgtgtta ggtaaatgat agacaatagc cagattttct
     8341 tgtatttctc ccacaacaat tccattgctt ttggcaaaac tgtaaggaat tttaagtgat
     8401 ttttcttcat tttccatgat ttatttggtc atttttgatg agtattgggg aggagtacta
     8461 aaaggtactg gtagattgcc ttgagttaat gggggcaaca ctgtttcatc atctgtttgt
     8521 tcaaaagcct cttgggagcg aatccaatcc agctgatatt gacggacata attgtatttt
     8581 tcgcctgtga catgcaagtt atctctttcg ttgcgtaata tgataggttt aataaatacc
     8641 atgagtactc gcttgtctct gttacgaata ttacgttgaa acaatctgcc tattccaggg
     8701 atatctccta aaatgggtaa tttattattg tcatttccaa tactgtcctg aatcaacccc
     8761 ccaagtacta caatatcacc actttctaca tgaactgaag ttacaatact gcttatatta
     8821 aaagtggggg ttgttgtgtt gtctgttgta gcagttgcag gatccaaagt atcattccct
     8881 tgatcaatct gtagttgaat tccttgtccg cgagtaattt gcggccttac ataaaggtgc
     8941 aacgcaacgt taaccctgtc aaatgttgtg aaaggacttg ctgtggtagt gcctcctgca
     9001 ttattggggt agctggttgt tgctattgag acctgtttac ctatgagtat ttttgcttga
     9061 cgattatcaa ggactactac ggagggagtt gataaaatat ttgctctttg ctctcttgcc
     9121 aaggcataaa tttgagcctg gaaatcgtca atcctggttt tactgtttat aatggcaaag
     9181 cctggtctga agtccttggg attgcctgtt tgttgatttg atccccactc tattcccaga
     9241 ttgttaacgt ccttttcatc cacttcagca acgagggcct cgattaatag ttgagcaggt
     9301 tttatatcta gctgagaaat gactgttttt agaatacgaa taattgaagc aggtgcatta
     9361 agaataatag agttagtgtt cggttctcct attatctgta cagtaggctt ggtagagcct
     9421 tcattttgag tacttgctga tgtcgtgttt gctgtcgctc ctgaggcatt cataggggca
     9481 gaactggtcg aagtggagct taatgaagag ctttgcccat cggcagaggt attggcaaga
     9541 ctggaggcag ggttggtgct gtctaatgcg ggtcttgtaa tggttccaat ggttgttcct
     9601 acatttccac taaaattggc ttgggctata cctgccagta tagggactaa atcttcagct
     9661 cttaaataat tcaggtaaac aacttgtgta tttgaattga ctcctgttga gctgtcttta
     9721 tccagtttca atatgagcat tcgcaaacga attctatccg ttttgctccc actgactaaa
     9781 attgagttgg aacggtcatc tgcagcaatg gtaatttgtg ttctgctgcc gataccaggt
     9841 tgagttttaa ccaggtcttt taaagtgttt gcaacatcca tagccaaagc atgatgtaaa
     9901 cgaaccatgt ctatgccgtt tgcacttgag ctatctactt gtttgataat ttccgcaagg
     9961 cttttaatat tgtttgctcg accagacaga atcaacatat tagaaggagc ataggcagat
    10021 acgctgctcc attggggcat tagtggcctt agaacaggta ccagttgttc agaaggaaca
    10081 taatgaaccg gtattacggc gaccatcata tcgtcacctc gtggaggact cttcattcca
    10141 ctgagtaaat caggtgactg tgtttttgca tcaatattgg gaataatttt gataatttct
    10201 ccattaggta ttgcggcata acctgatacc tgcagtactg agagaaagac ttgatatagc
    10261 tcgcggctgg ataacggcgt tgatgaaaca atcgatactt ttccttgtac tctgggatca
    10321 ataacaaaat ttttacctgt aattcgagag acttctgcga ttacggctct aatatcagcg
    10381 ttacgtaaat tccataattt actgcctgga ggagccgttt gcgcttgttc catattttta
    10441 ggcagttggc ttacctcaag tgaagaggtt tgatttttgc taattttatt aaactgtttt
    10501 tctgtttttt gagataactt ttctttgagt gctcttgcag tttggtaatc atcaattgga
    10561 ccaatctgca caaggtaaag atgtttgtca gccaggtttt taatttcaac tttttgatcg
    10621 actaatttgg caagttcatt ttggcgtatt ttagcgtctt tttcgagatt ataggcacca
    10681 gcttgaagat aaaaaaatgt attgccatct ttggttacgg ttgaaatatt gacatcatta
    10741 gcacgtacta atgaacaaca aacgaaggct aaaagtataa ttactatcga gcgcatttaa
    10801 aaaccgtaaa ataatggata atgacataat aactaaattt gactgcttac catagctttt
    10861 ttaataactt agagcaggaa atttaagtgg tttttactgc tatggtttca ctcgtgtcat
    10921 ttatgcgttt taatatacag tcagcaattt tttagccgga aggaatatat ggataatgcg
    10981 ataataaaat ggggaatttt aggtacaagt tttatctctg aagtgatggc taatgcaatt
    11041 caggaatcaa aaaccagtga gcttgttgct attgggagtc gaacaagcaa gatagccaaa
    11101 cgattttctg aaaaattcgc tattccaaaa ttttatgaca attatcaatc attaattctc
    11161 gatagtgata tagatgtggt ttatataggg ttacctaatc atctgcataa agaatggatt
    11221 attcgttgtg cgcaggcagg aaaaaatatt ttgtgtgaaa aaccttttgt tgttggtata
    11281 gaagaaatcc atgaggttat ttcagtaatt gagagaacaa atgttttttg tatggaagct
    11341 ctaatgtacc gatatcatcc ctttataaaa aaattacaag agatcattca aagcaacaca
    11401 ataggtaaga taaagttata taatgcgaca tatacagcaa acattgcaga aatagccaat
    11461 cctgttgcgg gaggaagtat acgtaatctc ggatgttacc caatttcctt agtgaggctg
    11521 cttgctaacg cagagccaat cgaaatccgt ggtattggaa gaatgaattc caggaataac
    11581 acggatagtc aagccagcgt tcttttaaaa tttgaagacg attcaattgc agcagtttcc
    11641 actgccgatg atattgaaat gcattggcaa ttcgaggttt atggaacaaa aggccattta
    11701 aaagtagaaa cgaatccctg gttgcctggc tctgagggta acaaaattta tatctctctc
    11761 aatactcaat caacacccag agaaataagc gtgaaagcag aaaagtcact ttatacctat
    11821 caaatagatt ttatcaatga gcaagtattg aaaggggatt ccagacaatt tagcaagacg
    11881 tctttattag atagcatggg aaatacaatc gttctggaaa cctggctaaa acaaatactc
    11941 tcttcaaaac caagccttga attatccaga ttaaaagaca ggctgcctca tgcataagca
    12001 aaaaggttct ggtgatacta gtcaaatttt ccgagaagag agtaatctgt ttttccttcg
    12061 cgccagatac catcccaatt gacaggtgga ttaactgcta ataccttgca gcgttcgatg
    12121 taaacggaag ctaattgatc gtcagagtag gctggagtaa gttttgcaaa cgcgcttata
    12181 ctttctttcc aggaaccttt ttgatagaga gaaaatgctt tttgaaattc ctttttatgt
    12241 cgctcaagat tttcaagatt ttgggctgta atcagttcat atatctcaat actttcacgc
    12301 ttacccctta cagccacttc atctaacaaa cggaaaggaa atttttcaga tacttgagtg
    12361 taagtggcgt gactgacaat gatttgagta tgataatttt tattaattga ttccaggcga
    12421 cttgccagat tgactgagtc gcccaaggct gtataactta gcctatcctc tgaaccaaca
    12481 ttacccacaa cagcatctcc ggtgtgaatg ccaattctga ttttaaattc gggaaatcca
    12541 agatgtttat tcttttgatt taacaggcta aggcgatcaa tcatgatttt ggcagccagg
    12601 catgcatgca atgaatgatt ggtatctttt gaaggtgcat tccataatgc catgatagca
    12661 tcgccaatgt atttatccaa agtcccttca tgttgtatta ctatctcggt catggactgg
    12721 aaataatcgg ataaataggt cattaatttt tgtggtgagg tggattctga aatacttgta
    12781 aaatctttta tatctgaaaa taaaatagta attgtttggc tttgccctcc aacttgagca
    12841 atctttccgc tacgcattaa tttttttacc aaagatccgg gaacatagcg ttggaatgaa
    12901 gctaagcttg atctcagaga tgatagtgtt ttatccatat aactgacttc tttaatcctg
    12961 gtcttcagaa gtggcctggg tttgaggtta agcatggtaa tttctttagc ttcttcagtc
    13021 agttgaataa taggatttga tattctttgt gaaacgatac gaactaataa tgtcccaatc
    13081 agcaagacca gtatagttaa caaaatatag cgcatgctta agtctttcag tggttctatc
    13141 acatctgttt caggaacaat aataataata tgccatagtg ctttttcatt tgtactggat
    13201 ataggttggt atgctaaata atatttttga ttatcatgag tatacgattt gatgatgggt
    13261 tcaaaatcat tgaaatcgag tttcttaagt ggaatattca gcttttttat ccattccggg
    13321 gttaatttct ttccacggat gtctttttta ttttgagggg ttctgaaagc aatgatattg
    13381 gactcattat taatcacata aatcatagtg tttttagtta attccagttc tcggataaaa
    13441 tgttgtaaac catcaatagt caggtcaaga gcaatcactc cttttatttg cttattttta
    13501 ttatatattg gtgaagcccc ggtaattcca gggataagga agtcactttt atcaaataca
    13561 taaaatgtat atacgtttgt ccatatcggt ttccctgctt ttatagcttg ttgataccac
    13621 gggcgaactc tgggatcata atggcgtgtc agcaattttt ttccaattat ttctccttgt
    13681 tcgtttaatt gataacgaac gttgactgga ggtgatacgg aattaatcac atgggttaac
    13741 ccaattattc ccttttgctc tctatctact cctataaaat caccagttgt tgaaccatag
    13801 taaaccataa atatttcagg attatatcga attgattcaa aaagaaattt atcaaattgt
    13861 tttgaatcat cagggttaat gatgccatta tttatggcat ttgttatttt aattaaatct
    13921 tgatttaaag gatttaaata gatctcaaac ctctctttga cgagtaatga tgtttgctca
    13981 attgaatctt tagcgctact gttaagaaca ctattgaaag caatataatt aataccaata
    14041 atagtgaacc ctaccaggct taacaaaagc acaaataagg taatgatgct gacacgtata
    14101 ctgattttaa aattactatc catatttttt ccttctaatt gaaggaagta cacaggttct
    14161 aagcgaaatg cgattaagaa cttattttaa ataagcatag tcttctttag gcagaaatac
    14221 acgcaaactt tatattttct taccaggatc aaagatcatg gttttttcag cagggctcca
    14281 ttcgttgaag ttttagaagg gcaagaaaac tcaaaatact taccaaactt aataagaaca
    14341 taaaagtttc tacttgctga attcctatca tgctggtaat aaaactaaag aaagcggagc
    14401 agttaaattg acaaaataaa gtgatagcta atgcaagagc catttgctga ggaaaagggc
    14461 gaacagctga tgctgttgct gatgcaacaa tgaaacctgt accaaaataa taaatcacca
    14521 taggcactat aaaagtcaaa tatatatttt cagcagaaaa gacaaataac cacatcaaga
    14581 atgctgcgaa aataaagaaa cgtactccta attttgttaa ttggatacta tgataatatt
    14641 ttgctattaa gccacaacaa gcggtaccta ataaatgcat aattgccata gtagtcttga
    14701 ttgtaccata ctgagcgctg gtaaaatttt ctcctttaat aagaataaaa gaggcgctgg
    14761 tattaaacgc cgattctcca gccatcatca aggcagaaat aaggaggtaa gaaacaaaaa
    14821 ggggatggtt gaagaccacc ataatactat gacctaggcg agggaatttt acataatggg
    14881 gtttggtttc ttttaaacct cgacataaag gtaataaaag caggccatag gcagcaatga
    14941 taagagaggc gatttgccaa ttccaataac tgtttatcaa acctccaaaa aatggaagaa
    15001 agcaaataaa cagcccagcc agagtaaata accacgcaaa gtattttgtg gcattttgtt
    15061 cattatgggt atcgttaatc attgctctac ccattagaag ggtagcgcct acgctaattc
    15121 cctggagaaa tcggcatact aaaaagggaa ggatggagtg gtttagagca atcagaaaat
    15181 tgctcagaat aaataacaac agtcctccaa tgaagattgg ttttcttcca taacgttcgg
    15241 aaagaagacc ccataaaatc atactgaggg cttttcccaa aagaaaaaga ctgatagtta
    15301 gttgaactgt attgggagtt accgagaagt aatcaactaa tgatggcaag gctggagtaa
    15361 ggaaatgtat atccatgttt ccaagaaacc aggttaataa cactataaga agagcatttt
    15421 ttttcatgat ttcacgttaa aataagaatt ggaaaaatct gtcatctgcc tgacatttag
    15481 ccgatcatac ccgtaaacaa aaatcgcgaa taaactataa attccggcaa ttatcataaa
    15541 aattgcagga tatggtagat aaaatgcaaa aagaccaccc aataacaatc caattagatt
    15601 tcccagtttt gcaaatgaat tggctaatcc caaggcataa cctggtaaca aattacgatc
    15661 actgcacaaa gcaaataacg caggcaataa ggctgcaagt acgatccccc acaaaatacg
    15721 tataagagca aacagataga aattatctac attggcttga atgatcatga ggatggctcc
    15781 gaatacacta tagcgtatta aatattggtt aaccaaagaa ggttgacttc gacagtagtc
    15841 aaactgtttg ccacaccata ctgaactaca tagcatcccc attgccggta aagaataaag
    15901 aaatccaatt agtaccagat tatttgaaca atacttattg agatacaggg taaaccctgg
    15961 atcaggtaaa aacttggcaa tttgggttag catgattaat acgcaaagaa agaagaaaac
    16021 actttttgat gtgtaagaat gatttaatgt ttgtgatctt ttctttatct gttgctttgg
    16081 tggggagggt aatttgtagt gcatcactac agtagtgccc aggcatatca atgttgccaa
    16141 accaaataat ccttgatagt tagtgaagct taaagctaat cctccgagaa atccggctgt
    16201 agaagtggca attgccttgg aggattgcaa gcgagataac tgtgtgcttt tgctttgcca
    16261 ttcagtaatg cttagggcat aggtttgcat tgcaacaaga aaacctgcaa aagctccttg
    16321 cagtaatcgg atgattaaaa tccagaatac agaattaaca aaaatcatac tggcctgggt
    16381 caacaccaag gcccaggatg ctctcattaa catgggttta tagccatatc gatcagctgc
    16441 caagccccag attggggtta atagaatgtt agccagcatc ggtagaacta gactcagcgt
    16501 gttataaatc actaccgatg gtactggtgt gttgctttgg tgcgcgatca atagtggcag
    16561 aaaaggattc gtcatttcca aggccaatat catcaaaaac tgacctgaga gcatcatttt
    16621 ttgaactagt ttattcatta tcttggctca atgggttgtc aagactaaaa aataccaggt
    16681 tatcctgatt ttgattgagt ctcatgctca gcaaactttt atgttgccag ggaagagaga
    16741 ataaatgttg ttggaatttt tggtatatca atggattaat ttcctttttg aaattttgta
    16801 ataattgatt tgcaatttgt tttagcagga tccaaaggtt ttttttagtc aagttatagt
    16861 tatcatgtaa acaattaatg caaaaagtta agttactcag taaatttcca taaatgaagg
    16921 tattaaagag atcactcaat tcgtggcatg aaatggtaga gtctggatga agattaggtt
    16981 tgggtgttgt ctcatatagc gaattaaaac agaccctgat gccgccaaga tctcgaataa
    17041 ctaatccaac aggccgatga gactgaaaaa tgactaaagt gttttgttgt tgggcttcca
    17101 acgcaacgcc atagcttaat aataaatgaa gctggcattc gagaatacat cgactgtatt
    17161 caataaaata tagttcaggc tccatgccac ttgcttcaat aatttcaacg aataaagcac
    17221 gttgactgac tggagaaata gcatagagtg ctgctacagg tactaatgtt tgtgacgcat
    17281 cgatcaattc cagtggattt tcacgtacta tcatagccaa atgcttttta ttttgtgggg
    17341 aaatggatgg gtgtgtggtg ttaatccccg ctaaatccct ggctagaaat agtggctttt
    17401 caaaactttg atactgagtc agtaattgcc taagccaact ggaaagagga gacgaattgc
    17461 ttactgaggc aggagataca gtgcgtaaag cagaagtagt atggattgct gtagctaatt
    17521 ttaagtgtgg acctttgtta tctaaaggca tcatcgttcg gaatgacatg gatggtttgg
    17581 ttttttgaac agcctctgtt attaataatt gctttgaatt gattaataca gaggccagtg
    17641 tttgtaattt gttattccat tgccagggat gaactggaag aggcaaatac tgatcaggat
    17701 ctaattgttg tttttggaca gaataattcc acaattgata ttctttagca aaatgttggg
    17761 caaacaacaa agaatagcta gtcgcatcaa ttgaagtaaa cgctacggag cgatgaattg
    17821 ccagccaggg aatatctacc tgcgcgttaa actcagggga gtatcgaatc acttcttcag
    17881 gaataaaccc catttttgcg cggaaattag gatgatacgg gtgccctatg caaccccatt
    17941 gctctaaaaa agtcagaatt tgttgtttat tatgttgctc tgataaccat gaccataaac
    18001 tggagtggtt tttcgcctgt tgcttcagcg attgttgcca gcaatgataa taagcgaggg
    18061 ctaacgcctg attgttgatg ctttcatcga gttccttttt tagaagaggc catcgatcaa
    18121 atgttttttc cggtttttgc tttt
//
LOCUS       DACTJE010000053        18007 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.053, whole genome shotgun sequence.
ACCESSION   DACTJE010000053 DACTJE010000000
VERSION     DACTJE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18007
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.053"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1019)
                     /locus_tag="I9760_001403"
     CDS             complement(<1..1019)
                     /locus_tag="I9760_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HAT5043176.1"
                     /translation="MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGQVPIKS
                     NQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGRFRINLFRQRGEVAMVVRYIKGE
                     IPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
                     PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYA
                     ETGHLCISTLHANNANQALDRVINFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQR
                     VAAVEILINSPYISDLIEKGKIDDIKEVMARSAEQGMQTFDQALFNLYKEGKITKENA
                     IKYADS"
     gene            complement(1109..3037)
                     /locus_tag="I9760_001404"
     CDS             complement(1109..3037)
                     /locus_tag="I9760_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-gulono-gamma-lactone oxidase"
                     /protein_id="HAT5043177.1"
                     /translation="MALPTELKSKFIQILNQNKISHSVGWSNYMESVNNPAAIVVEVK
                     NEKEVQTVMKAIKELNSDREPKDKVTVRATAGWTDKPGKSCCFFPWSEAQESQYNEGF
                     SFSQVVGGKASPDTAGTDVIIRFAKKFHHAKVIGPLKNPPVFNPDNPIHRLPMTLVEV
                     SAGMQVADFADFLREHQLSLSTVSMISWVTPVGLAGTAGHGTGRDEPAFSGLIESVTV
                     CDMDGNLREINSDHPDFKDLTGGHSGLLGIVVSMKLRAVKAFNLCETIELFPNTREMT
                     GRLSDILKNNQYISIMGMPSYGCPESDKLVSKWQIRQWNYTTDKPGKKEKAPYDPDVR
                     SFAQELQVRIGASVMDFLLDSGLKHLLPSFMLLSAAVVTGTRGTSPKVDYENHITHPQ
                     VAFPKEMRDVSYLIPVKDDEAGQKLELILQKMEAMLNEAGSMGEFPVTYAIYVRYLKG
                     TNGGLSTSFTSSDDERILAIDVVTHPEAPGITNFEKGFMSFLKEMNIIPRNHLGKNFP
                     AGVVHYDQFLGAERVSKYKQALERFYCSPGKNDGAERLSMSPFYTPYLQGMLSASAPT
                     NQLVDQNKYEQRIEKLHEEHSDLECAEFLSKLYAEVSRMSTFSEEALTAKKGFLEACK
                     AELEHRNSRLEAPVPLLS"
     gene            complement(3325..4125)
                     /locus_tag="I9760_001405"
     CDS             complement(3325..4125)
                     /locus_tag="I9760_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase"
                     /protein_id="HAT5043178.1"
                     /translation="MNEDTIRDFNRNGAVCLRQFLNAEEIAILKEGIEFNLSSLSPRA
                     KIASRPDDPGYFVEDFCTWQNNPFYQRIIFGTNLGVAAAQIMQSQSVRLYHDHLLVKE
                     PGTRQVTPWHQDQPYYNIEGKQNCSFWIPVDPVSRSSTLEFIAGSHLGPWLMPRTFLD
                     NQAKWFPEGSLQELPPIEEARARYPIIGWEIEPGDVVCFHMLTLHMARGVEGRHRRRV
                     FSVRFLGDDITHAPRAWPTSPDFPGLTAEIPAGASMDHPLFPCLPLKI"
     gene            complement(4158..5510)
                     /gene="radA"
                     /locus_tag="I9760_001406"
     CDS             complement(4158..5510)
                     /gene="radA"
                     /locus_tag="I9760_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAT5043179.1"
                     /translation="MKVKTQFVCNQCAAIFKQWAGQCTQCGAWNSIVEEGIMPENKTL
                     RSGQWANQRSVITAVEDVVMDKIVRMDCGISELNRVLGGGLVYGSVVLIGGDPGIGKS
                     TLLLQTLANLSMQETVLYVTGEESLQQVAMRAQRLGLPLAGLRLLAETQVESIIAQAQ
                     KEKPRIIVVDSVQTIFTEAISSAPGGVSQVRESAAQLVRYAKMTNTAIFLVGHVTKEG
                     TLAGPRVLEHMVDSVLYFEGQNDSRFRVIRAIKNRFGAVNELGVFAMTDKGLKEVANP
                     SAIFLSRQPEPTPGSAVMVTWEGSRPMLVEVQALVDEAHGQQAKRVTAGLEHNRLAIL
                     LAVLHRHGGIATYDQDVFINVVGGVKVTETASDLALLAAVISSLRNRIFDSETIIFGE
                     VGLAGEIRPVQSGQERIKEAIKHGFKRAIVPFANAPKQQIDSMTVEPVKHLRDVLEKM
                     "
     gene            5745..6638
                     /locus_tag="I9760_001407"
     CDS             5745..6638
                     /locus_tag="I9760_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2327 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043180.1"
                     /translation="MAKFTVGQDPVKGLTELKAYMEEQISKIKKATSEQEIDQLLVEV
                     LNEYDDKMGSLSKIYKGGNEQVEQLKIDVRKLYEPLRDQFSYNHPQTLVGEFQTKLEE
                     QHKRAEEEKRKLEKQREEQLKLEKQLEEEKQKLAKQSEVEEKPKLENQQEENITQALQ
                     KVDGIIQELTLKIDRVDQHQYKKAHDTANTLLQSLIAARDEYERDLRANEFSQELAGR
                     KFKLACQDAVKIAKPVLEKDLGWGDYLKNLLKCLGNAVITVFTFGYQQGFFAYARPDS
                     AKAVEKAEEDLGLRQAASSPK"
     gene            6902..7285
                     /gene="lem22"
                     /locus_tag="I9760_001408"
     CDS             6902..7285
                     /gene="lem22"
                     /locus_tag="I9760_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem22"
                     /protein_id="HAT5043181.1"
                     /translation="MPAFKKGQNPVTELTRLKEYMEDQIAKAKESSSLTAQLKFLENA
                     HTEHFVKMGSLTTIYKGGSEVVDRLKIEIRSLYEEMLELKDKCRDQIQQYETSIKNSP
                     TFFTTRNSKTKDEEFDLDFDKAFGL"
     gene            7479..7709
                     /locus_tag="I9760_001409"
     CDS             7479..7709
                     /locus_tag="I9760_001409"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAT5043182.1"
                     /translation="MSKGIHFEQSITELEEIVRQLEKGELSLEESLKQFEKGISLARR
                     CQNALNQAEQKIETLTGTDSNIELDSDEQTSD"
     gene            7690..8586
                     /locus_tag="I9760_001410"
     CDS             7690..8586
                     /locus_tag="I9760_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAT5043183.1"
                     /translation="MNKQVIDKYTQRHELYLEQLLNEIIIPAPQIRSALHYALFSGGK
                     RIRPILVYLAGDLIDVDQGVLDIIAAALELTHCYSLIHDDLPAMDNDDLRRGKPSCHK
                     AFDEATAILVGDGMQALAIEVLLMRLSPLLPAAQVVAITQVLVNASGISGMVSGQSLD
                     LSELAKSSVTEEQLREIHLLKTGKLILACFEMVLAAQHEVSEQIKSALRTYGKHIGLV
                     FQMQDDYLDLYAPTQILGKGRSSDQANQKTTFATLFNKQQLEEEIAVHYQIAMDSLRL
                     FGSKAAALIELTKQLQNRSNLS"
     gene            complement(8630..9484)
                     /gene="bioC"
                     /locus_tag="I9760_001411"
     CDS             complement(8630..9484)
                     /gene="bioC"
                     /locus_tag="I9760_001411"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAT5043184.1"
                     /translation="MTVNNEISKAFSKHADDYERVAKVQKEIGSRLFERLQYLKIAPR
                     RILDLGCGPGFFSKELALLYPKAQIVGMDLSFAMLEQARKKQGWRRKWPLVSADMQKM
                     PFATGAFDLVFANQVIHWSSSLGMVFRELNRVMNVNGCLMFTTLGPDTFKELQTAWSA
                     ANQYAHVNEFVDMHDIGDCLIAEHFMDPVIDMELLSIHYETLPQLLLALKTQGVRNIN
                     PKRNHGLTGKSAWKQFEAQYATMRTTTGKYPLTYEVVYGQAWKGAQRKMEQGIETWIP
                     VSRVVKKS"
     gene            9642..10232
                     /locus_tag="I9760_001412"
     CDS             9642..10232
                     /locus_tag="I9760_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAT5043185.1"
                     /translation="MKQLGSCCQYCAYPLSDDTYLVCGQCVRKRPSFDSAYIAYRFEE
                     PLRSLIHQFKYHNGLYLASFLKQLLLNALPKNALKPDCLIPVPMHPKRLKRRGFNQAG
                     VLTRLLARQLNIPYDLYYCQKIINTASQANLDGEQRRKNLRHAFYVSAVTYEHVMIVD
                     DLLTTGSTANEMAHTLKNAGVKRVDICCCARAVTKN"
     gene            complement(10261..11334)
                     /locus_tag="I9760_001413"
     CDS             complement(10261..11334)
                     /locus_tag="I9760_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAT5043186.1"
                     /translation="MIMALVAIILTLILVIGIHELGHALAARLFQVKISKISIGFGKP
                     LIQWQTQSGCNWIWAMWPLGGYVQLLNSRISPVKPQENAYCFDKKPIWIRVLILLSGA
                     IANLITAWIALVLVFYIGISYKQPQIQSVKLDSLAAKAGIQAGDQWVAIENYPTDSWQ
                     GVGMQLVIHWGQKDVHIVLRQGNQQLKQLNLDLSQIEFTSKDGSLLESLGIKPDLSAV
                     SSLTRYPSLLASMQKAFAEIIHTMYFFIMILKQLFLGVIPFSILLGPLAIFSVSVASL
                     TQGVIVFLLFIATLSLAVALVNLFPIPGLDGGSILYSFIEKIRGKPVSVAVEVLLHRL
                     MIILFCVLLVHLLMNDLNRYLHH"
     gene            complement(11391..11669)
                     /locus_tag="I9760_001414"
     CDS             complement(11391..11669)
                     /locus_tag="I9760_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAT5043187.1"
                     /translation="MDIILQGQHNAIETVESLERVLKLFKERYQVDGFREIHLTVTLV
                     DEQGDEVELVDSDTNQPYRIFEVYRKGYELAGRKGIPMLQLVVDNTHK"
     gene            11987..13261
                     /locus_tag="I9760_001415"
     CDS             11987..13261
                     /locus_tag="I9760_001415"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAT5043188.1"
                     /translation="MVFVACGLNHKTAPIHVREKVALQPAMQDSLLSSLLDLPEVNEA
                     AILSTCNRTEIYCDTNTPEVLGNWLAHEHQLSEELLSQFLYIHQGKEGIKHTLRVASG
                     LDSMMIGEPQILGQMKQAYQHACRLGTVKTQLRPVFEYIFRASKRIRTRSGIGANPVS
                     IAYAAVQLIGQLFKNYHSLSVFLIGSGETASLVAKYLHQHGVHRFLIASRTLENAQKL
                     AETFGGKTLSIGDIPQYLPLADVVISATACPLPFINKSLVEHALEQRNHAPMFLLDLA
                     VPRDIEGNVNELEQVHLYNVDDLQSMIEKGMDERRNAALQAEQLIESELDNYIRWHRS
                     LRAKDVICDYRNQMHTLAQQELQRALKKISAGQNQQDVLNEFSMRLVNKLTHNPTIGL
                     RQIAWDNREDLLDLARYLFDTTANQSLYEEIS"
     gene            13245..14333
                     /gene="prfA"
                     /locus_tag="I9760_001416"
     CDS             13245..14333
                     /gene="prfA"
                     /locus_tag="I9760_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAT5043189.1"
                     /translation="MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQ
                     LEPVSQCYESYLEAKNNLDSLNELLESDDKDLATMAEEEIDTVKKQIEELDEQLQWHL
                     IPKDPDDERNIYLEVRAGTGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGG
                     YKEIIAKISGQAVYSQLKFESGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQ
                     INPDDLRIDTYRSSGAGGQHVNKTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLL
                     KTRLLDAEVSKQKQEQAQTRKSLVGTGDRSERIRTYNFPQGRLTDHRINLTIYQLSDI
                     MEGNLSLVIDPLKREYHAELLADLGRHD"
     gene            14326..15189
                     /gene="prmC"
                     /locus_tag="I9760_001417"
     CDS             14326..15189
                     /gene="prmC"
                     /locus_tag="I9760_001417"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAT5043190.1"
                     /translation="MIDIRSLLEQALQQFKVKNQETKLEAELLLCHVLNKNRAYLFAH
                     PDALVSPEQIETYLQMIKQRAEGLPIAYITGQREFWSLSLKVTPNVLIPRHETERLVE
                     LALELIPDKENVSVLDLGTGSGAIALALAKERPLWHIDACDFSKEALELARYNAKTLG
                     LNNINFCHSYWFNNLPLKQYHAIVSNPPYIAENDPHLKHGDVRFEPTSALVSSQDGLA
                     DLQYIIQHSYEYLLPDGLLLVEHGFEQKNEISAILNQLGYKNIHCWQDLQGHDRVSGG
                     QRPSNRNNKPL"
     gene            15288..15764
                     /gene="dksA"
                     /locus_tag="I9760_001418"
     CDS             15288..15764
                     /gene="dksA"
                     /locus_tag="I9760_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HAT5043191.1"
                     /translation="MTGQLIDKTKERLYNVKNDAIGNMGIAPYQETEGEEYMNEKQLA
                     HIEKILLAWRQSLMEEVDRTVSHMKDEAANFPDPSDRASQEEEFSIELRTRDRERKLI
                     KKIEDALERLRNEDFGYCEACGIEIGLKRLEARPTATLCIDCKTLSEIKERQNQGA"
     gene            15834..16667
                     /locus_tag="I9760_001419"
     CDS             15834..16667
                     /locus_tag="I9760_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5043192.1"
                     /translation="MFFMYFTNYGYRLNQIKKGSDQHWLFLPGGPGLGSEYLASFCDK
                     LTLQGTVSLVDFPKDGNNSEGQLDFVSWQEGLISLLKSLNKPILVTHSFSGMFTLNFP
                     EIENHLSGLVLMNTTTTNSFFQHVNEMRQKHNLPDLVPAASQYHLYPSNETYKKFWDT
                     YKHYCFTQEELPIGEQMMELFAYNNESYHYAIEHFYNDFHYKWHPQTIPTMTIASELD
                     YICPPAIFIQDKNFQCQNVINKVIYKAGHCPWILHLEQVQKCFDEFSGMIQEINIERI
                     L"
     gene            complement(16672..17931)
                     /locus_tag="I9760_001420"
     CDS             complement(16672..17931)
                     /locus_tag="I9760_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAT5043193.1"
                     /translation="MRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQE
                     NAPVDVWLHAVSLGEVIAATPLIDAMLNKHWSVLVTTMTPTGSERVKSRFGHKVAHQY
                     LPYDLPWVLKRFFKRTKPRVGIIMETELWPNLIKQAHASGVALFLANGRLSDRSLQGY
                     LKLKFLFKPVLNQFSGILTQSNEDAERFIALGANADLVHVLGNMKFDLQIDSVDSSQY
                     RELKSHWGEDRLTVIAASTHDDEESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQ
                     LSVQAGFNTGCRSNLDTISRENEVVILDSLGELLGFYQISDFAFVGGSLVPVGGHNVL
                     EPIAMNVPVLSGNQVHNFKSICRELKEAQAILLVNHANELVDAIIKLYQDKESQSTMV
                     ANASSVLESNKGSVVRYLQKIESVLGY"
BASE COUNT         5739 a         3858 c         3295 g         5115 t
ORIGIN      
        1 gaatcagcat acttaatcgc attttctttg gtaatttttc cttctttata taaattaaat
       61 aaagcctgat cgaaagtctg catgccttgt tccgcgcttc ttgccatgac ttctttaata
      121 tcgtctatct tccctttttc aatcaaatcg gaaatgtaag gagagttaat caagatctcc
      181 accgctgcga cacgttgatt atcaactcct ggtatcaaac gtaatgaaat gattgctttt
      241 aaattaagag ataaatctaa taatatctgt tgtcttgcct catcggggaa aaaattaatc
      301 actctatcca gagcctgatt ggcgttattg gcatgcaaag tagaaatgca caaatgtcct
      361 gtttcagcat aagctatcgc atgtttcatt gagttaagat cacgaatttc accaattaat
      421 atcacatcag gcgcttctct catcgcgttt ttcaatgcat tgtcatagct tagagtatca
      481 atacctatct ctcgttgatc aactattgat tttttatgtt gatgtatata ttcaatagga
      541 tcctctattg ttaaaatatg ccctctgtga ttttcattac ggtggttgat catcgcggcc
      601 agagtcgttg atttacccga tcccgtggac ccaacaacca atactaaacc acgcagctcc
      661 aagaccagag actttaaaat ttgaggaaga tttaaatctt ctatagaagg tatttcgccc
      721 ttgatgtaac gcaccaccat agccacttcg ccacgttgcc tgaataaatt gattcggaaa
      781 cgccctactc cagctaatga aaaagccata ttgagttcca ttttagactc aaattctttt
      841 tgctgtgcgt cattcataat agataaaaca atttctgcca tttgattcga tttgatagga
      901 acctggccaa taggtgatgt gatgccctcg attttaatat ttggtggtgc cccgacactg
      961 aaaaacaaat ctgaggcacc tttatcgacc atgagcttaa aaaaaggagt aatatccata
     1021 ggttaactca aaatattatt atatttttaa gtttagtaga gactcctgaa tttgctaggc
     1081 tctgccttgt gcttattttc ctgattgctt aactcaatag aggtacaggg gcttctaatc
     1141 gactgttcct gtgctccaat tccgccttac atgcttctaa aaaccctttt tttgcagtaa
     1201 gagcctcttc tgaaaacgta ctcatcctgg acacctcggc atataattta ctcaaaaact
     1261 cagcacattc gagatcagaa tgctcctcat gcaacttctc tatgcgttgc tcgtatttat
     1321 tttgatcaac taattgattg gtaggtgcac ttgcagataa catgccttgc aaataagggg
     1381 tataaaaagg agacatgctc aggcgctcag caccatcgtt ctttcctgga ctgcaataga
     1441 atctctccag tgcctgctta tactttgaca ccctctccgc acccaaaaac tgatcataat
     1501 gcactacgcc cgcggggaaa ttttttccca aatgatttct gggaataata ttcatctctt
     1561 ttaaaaaaga catgaatccc ttctcaaaat ttgtaatccc aggtgcttca ggatgagtga
     1621 ctacgtctat cgctaaaata cgctcatcat ctgatgaagt aaaactggtt gataatcctc
     1681 cgttagtccc cttaagataa cgaacataaa tcgcataagt aacaggaaat tcccccatac
     1741 tacccgcttc gttcaacatg gcttccattt tttgtaaaat aagttccaat ttttgccctg
     1801 cttcgtcatc tttgactgga atcaaataac tcacatccct catctccttt ggaaaagcca
     1861 cctgaggatg tgtgatatga ttttcataat ctacttttgg tgatgtacca cgagtacctg
     1921 tgactactgc tgctgacagt aacataaaag aaggtaataa atgctttaat cctgagtcca
     1981 gtaagaaatc catcactgag gcaccaatgc gaacctgtaa ctcttgagca aaagagcgaa
     2041 cgtccggatc ataaggggcc ttttcctttt ttcctggttt gtcggtagta taattccatt
     2101 gacgaatttg ccatttggaa accagtttat ccgattcagg acagccgtaa cttggcattc
     2161 ccataatact gatgtattga ttattcttta atatatcact taacctgcct gtcatttccc
     2221 tggtattggg aaataattct attgtttcac ataaattaaa tgccttaacc gctcgtaatt
     2281 tcatgctaac aacaataccc aacaacccgc tatgaccacc tgtcaaatct ttgaaatcag
     2341 gatgatcaga attaatttca cgcaggttgc cgtccatatc acaaactgta accgattcaa
     2401 ttaggccact gaaagccggt tcatcacgcc cggtaccatg tcccgctgtt ccagccaagc
     2461 caacaggggt cacccaggaa atcatgctaa cagttgacaa ggataactga tgttctcgta
     2521 aaaaatcggc aaaatcagcc acctgcatcc ctgcactcac ttccaccaaa gtcatgggga
     2581 gtctatgaat gggattgtct ggattaaaaa cagggggatt ttttaatgga ccaatcactt
     2641 ttgcatgatg aaattttttt gcaaaacgga taattacatc ggtacctgcc gtatcaggag
     2701 atgctttacc acctacgact tgagaaaaag aaaatccttc attgtactga ctctcctggg
     2761 cttcgctcca tggaaaaaag caacagcttt tacccggttt atcagtccat cccgcagtag
     2821 ctctcaccgt cactttatct ttgggttccc tgtcagaatt caattcctta atcgccttca
     2881 tcactgtctg aacctctttt tcatttttaa cttccacgac aatcgcggct ggattattaa
     2941 cactctccat gtagttagac caaccaacag aatgagaaat tttgttttga ttcagaattt
     3001 gaatgaactt actttttaac tccgtaggta acgccataat tactccttgt aatattagtc
     3061 ctcaaacggt taaacataag tttaatagag caaaccgatg taccatgaac agtgcctctc
     3121 gatcaggtat acataaagac acttcaaaaa tacaatacaa tacttaatga aaaacaataa
     3181 cgaattttgg tagtcaataa taaaaaatat acccttttga ttgtacgctt cttggaataa
     3241 catccgttgt gaataggaat cttaaaaatc ttatccttga aaaaatgagc tcttttttga
     3301 agggaaataa aatatatcgt tttactatat tttgagtggc aaacatggaa ataatggatg
     3361 atccatgcta gcgcctgccg gtatttcggc cgtcaaacca ggaaaatcag gtgaagtagg
     3421 ccatgctctg ggggcatgcg taatatcatc acctaaaaaa cgcacagaaa acactcttct
     3481 tctatgcctt ccctcaactc ctcttgccat gtgtaaagtc agcatatgga aacatacaac
     3541 atcgcctggc tcaatttccc atccaataat tggatacctt gctcgcgcct cttctatagg
     3601 aggcaactcc tgcaaacttc cttcaggaaa ccattttgcc tgattatcta aaaatgttct
     3661 tggcattaac cagggaccca aatgagaacc agcgataaat tccagagttg aagaacgact
     3721 gacaggatca accggaatcc agaaactgca gttctgcttc ccctcaatat tataatacgg
     3781 ctgatcctga tgccagggtg taacctgtcg tgtacctggt tcctttacca acaaatgatc
     3841 atgatacaac cggactgatt ggctttgcat tatttgagcg gcagctactc ccaaattcgt
     3901 accaaagata attcgttgat aaaaaggatt gttttgccaa gtacaaaaat cctcaacaaa
     3961 ataaccagga tcatcgggtc tgctggctat tttggcacgg ggacttaagg aggataaatt
     4021 aaattctatt ccttctttta gaatagcaat ttcttccgca tttaaaaatt ggcgaagaca
     4081 tacggcacca ttacgattaa aatcacggat agtatcttca ttcattttat attccggtgt
     4141 gattggtttt tgataactca cattttctcc aatacatctc gcaaatgttt aactggctct
     4201 accgtcatgg aatcgatttg ctgtttggga gcattggcaa aagggactat agcacgcttg
     4261 aatccatgtt ttatagcctc cttaatgcgt tcttgaccac tttgcactgg tctgatttcc
     4321 cccgctaatc ccacttcacc gaatatgatg gtttcactat caaatatacg attacgtaaa
     4381 cttgaaatca ctgcagccaa caaagccaaa tcactcgctg tttcagtcac tttcactccc
     4441 ccgactacat tgataaaaac atcctgatcg taagtagcga tacctccatg acggtgtaaa
     4501 acagctaaca agatagccag gcgattatgc tcaagacccg cagtaacgcg tttcgcttgc
     4561 tgaccgtggg cctcatcaac caatgcttgt acttctacca acatgggtct tgatccttcc
     4621 caagtcacca tcactgcact gccaggcgta ggttcgggct ggcgagataa aaaaatagct
     4681 gaggggttgg ctacctcctt caggcctttg tcagtcatcg caaatacacc taattcattg
     4741 acagcgccaa aacgattttt aatggcgcga atgactctga agcggctatc attttgcccc
     4801 tcaaaataga gaacactatc aaccatatgt tctaacaccc gaggcccggc taatgttcct
     4861 tccttggtga catgccctac caggaagatg gccgtattgg tcatcttggc ataacgcacc
     4921 aattgtgctg ccgattccct gacttgacta actcctcccg gagcagaact tatggcttca
     4981 gtgaatatcg tttgtactga atcgacgaca ataattcgcg gcttttcctt ttgggcttga
     5041 gcaatgatgg attcaacctg agtttctgcc aatagccgca gtccggccaa aggtagtccc
     5101 aatctttggg cacgcatggc aacttgttgt aatgactctt cccccgtcac atacaatact
     5161 gtttcttgca tagataaatt agccagcgtt tgcaacagta aggtggattt accgatcccc
     5221 ggatcccctc caatcaaaac aaccgagcca taaaccagcc cccctcccaa aacccggttg
     5281 agctcggata taccacaatc catacgtact attttgtcca ttacaacgtc ttcaacagca
     5341 gtgatgacag agcgctgatt agcccattgc ccacttcgta aagtcttgtt ttcaggcata
     5401 atcccttctt caacaatgga attccaggcg ccacattgag tacattgtcc tgcccattgt
     5461 ttaaaaatgg ccgcgcattg attacaaaca aactgggttt tcactttcat aatttttgcc
     5521 ttgattcaag cattttattt agtacattta tgataactcg cgctgaacat atttgcctta
     5581 aataattttt aaatgaacca ggacttatct tatcttgaac gccacctttt tctttttatt
     5641 gcactcaatt tacaaaatat ttattaaaac tttacttttc aaaaacactt tcttgcttta
     5701 ttatttgccg aagtgatttt tttataaaaa ccgaggataa aattatggca aaattcaccg
     5761 taggtcagga tccagttaaa ggtttaactg aacttaaggc atacatggaa gagcaaattt
     5821 caaaaattaa aaaagcaacc tcagagcagg aaatcgatca actacttgta gaggttttga
     5881 atgagtacga tgataaaatg ggttcattat caaaaattta caagggtgga aatgagcaag
     5941 ttgaacaact aaaaattgac gtaagaaaat tatatgaacc tttacgtgat caatttagtt
     6001 ataaccaccc acaaacactg gttggtgaat ttcaaacaaa attagaagaa caacacaaac
     6061 gggctgaaga agaaaagcgt aagctggaaa aacagcgcga agaacaattg aaactggaaa
     6121 agcaacttga agaagaaaag caaaaacttg caaaacaatc tgaagtagaa gaaaaaccta
     6181 agcttgaaaa tcaacaggaa gaaaatataa ctcaagctct ccagaaagtt gatggaatta
     6241 ttcaagagct cacactcaaa atagacagag ttgatcaaca ccagtataaa aaagcacatg
     6301 acacagcaaa tacacttctc caaagtctta tagctgctcg tgatgagtat gagcgagact
     6361 taagagccaa tgaattttct caggaacttg ctggaagaaa gtttaaatta gcttgtcaag
     6421 atgccgtaaa aatagctaaa ccggttttag aaaaagactt aggctggggt gactacctga
     6481 aaaatctttt aaagtgcttg ggcaacgcgg tgattaccgt ctttacgttt ggttatcaac
     6541 aaggtttctt cgcttatgca cgtccagatt cagcgaaagc ggtagaaaaa gctgaagaag
     6601 atttggggct tcgtcaagca gcttcatcac ccaaataatt aaacttcaaa caaaaacctg
     6661 acattcgctt ggcttcacga tgtttaaatg cgaatgtcag gttttttata caaatcgcgc
     6721 ctgattttca tagacttatc tgacaaacaa tttggcttca atttaaattg attaacccta
     6781 taccaggcac tatgcattaa tacttatcta ataaaaaaga atatgaaaat atttgctttt
     6841 caattgcctg tttttgcttt aacttaccta agggattatt ttaaaatgtg aggtcaaaat
     6901 catgcctgct ttcaaaaaag gacaaaatcc ggtaacagaa cttaccagat taaaagagta
     6961 tatggaagac caaatagcga aagcaaaaga gtcctcctct ttaacagctc aactaaagtt
     7021 cctggaaaac gcccacacag aacactttgt caaaatgggc tctctaacca ccatttacaa
     7081 gggcggcagt gaagtagttg accgcttgaa aattgaaata cgctctttat atgaagaaat
     7141 gctcgagtta aaagataaat gccgtgatca gattcaacaa tatgagacat ctataaaaaa
     7201 cagccccacc ttcttcacta cgaggaatag caaaacaaag gatgaagaat ttgatctgga
     7261 ttttgataaa gcatttggtc tttaaacgct gttaactacc taatgttggt ctttgatatt
     7321 aaacttgcaa agtccaacat ccagttctgg agttccttta taaaacccaa agctgccaat
     7381 aagcttctat gattgatttt ccacttactt ccattcagaa gttgaatcct cgcgttaacc
     7441 cagtataatc acacctttta tcgccttgga aaacagttat gagcaaaggc atacatttcg
     7501 aacaatcaat caccgaactc gaagaaattg tccggcaact ggaaaaggga gagcttagcc
     7561 ttgaagaatc gctgaaacaa tttgaaaagg gaattagcct tgcaagacgt tgccaaaatg
     7621 ctttgaatca ggcagagcaa aaaattgaaa cgttaactgg tactgattcc aacattgaac
     7681 tcgactcaga tgaacaaaca agtgattgat aagtataccc aaagacatga gctatatctc
     7741 gagcaattgc tcaatgaaat aataatacca gctccccaaa ttcgctccgc actccattac
     7801 gctttatttt caggcggcaa aagaataaga cccattttgg tttatttggc tggcgattta
     7861 attgatgttg accagggagt tctggatatt attgcggcag cgcttgagtt aacacattgc
     7921 tattccctga ttcatgatga tcttccagcg atggacaatg acgatctgcg ccgcggtaag
     7981 ccgagttgtc ataaagcatt tgatgaggca accgctattt tagtcggcga tggcatgcaa
     8041 gctttagcga ttgaagtctt actgatgaga ttatcgcccc ttctccccgc agcacaagtc
     8101 gtagccatta ctcaagtatt ggtaaatgcc agtggaataa gtggaatggt tagtggccaa
     8161 agtcttgatt tatcagaact ggccaaatca tcggtaactg aggagcaatt aagagaaatt
     8221 catcttttga aaactggaaa actgatcctg gcgtgttttg aaatggtttt agcagcacaa
     8281 catgaggtat ctgagcaaat caaatccgca ctaagaactt atgggaaaca tatagggctt
     8341 gtatttcaaa tgcaagatga ttacctggat ctgtacgctc caactcaaat attgggaaaa
     8401 ggtcgctcct cggatcaggc aaaccaaaaa accacttttg caaccttatt taacaagcaa
     8461 caattggaag aagaaatcgc tgttcattac caaattgcaa tggattctct gcggttattt
     8521 ggtagtaaag cggctgcatt aattgaatta acaaaacaat tacaaaatcg tagcaatttg
     8581 tcttgacacc attgatgcac cggacaatcc gattgatatc cgttttaaat taggattttt
     8641 taacaacacg cgaaacagga atccaggttt ctataccttg ctccatttta cgttgcgcac
     8701 ccttccaggc ttgcccataa actacttcat aagttaaggg atatttgcca gtagtagtac
     8761 gcattgtagc gtattgtgct tcaaattgtt tccatgcact tttacctgtg agcccgtgat
     8821 ttcgtttagg attaatattt cttacccctt gagtttttag agctaatagc aattggggaa
     8881 gggtctcgta atgtatagac agtaattcca tgtcaatgac tgggtccatg aaatgctcgg
     8941 caatcagaca atctccaata tcatgcatat caacaaattc attaacatga gcatattgat
     9001 tagcggcaga ccaggccgtt tgtaattcct tgaaggtatc aggacccaag gtagtaaaca
     9061 ttaagcatcc gttgacattc attacacgat taagttcccg aaaaaccatg cctaatgagc
     9121 tactccaatg aataacctgg ttagcaaaaa ccaaatcaaa agcgcctgtt gcaaaaggca
     9181 ttttctgcat atcggcactg actaaaggcc atttacgacg ccaaccctgt tttttcctgg
     9241 cttgctctaa catggcaaac gacaaatcca ttccaacaat ctgcgctttc ggatataaca
     9301 aagccaattc ttttgaaaaa aatccaggcc cacaccccaa atcaagaatt cgtcgaggag
     9361 caattttcaa atattgtaat cgctcaaaca aacgactgcc gatttctttt tgaactttgg
     9421 ccacacgctc atagtcatcg gcatgtttgc taaatgcctt gcttatttca ttgttgaccg
     9481 tcattataaa gaccagggaa aaattaaatg ctggaaatcc aactatcgtg ctccaaaaat
     9541 tattaagtat aacacagaac ttacgtttac cttcaatatg cactctttgc aaccagttcc
     9601 acaaaagcca acttgcagtt tgctctaatt gcatggagtt tatgaaacaa cttggatctt
     9661 gctgtcaata ctgtgcctac cccctttctg atgatacgta tctggtttgt ggtcaatgcg
     9721 ttagaaaaag gccctccttt gatagtgctt atatagcgta tcgttttgaa gaacctctgc
     9781 gtagtttaat acatcagttt aaataccata atggccttta tctggcctcg tttctaaagc
     9841 aattacttct taatgcctta cccaaaaacg cattgaaacc agattgtcta attcccgttc
     9901 ccatgcatcc caaacgatta aaacgaaggg gatttaacca ggctggtgta ttaaccagat
     9961 tattagctcg tcaattaaac atcccttatg atttatatta ttgccaaaaa ataatcaata
    10021 ccgcttcaca agccaacctt gatggagagc aacgaaggaa aaatttacgt catgcttttt
    10081 atgtatcagc tgtaacttat gaacacgtta tgattgttga tgacttatta accacaggca
    10141 gcacagccaa tgaaatggca catacattaa aaaatgctgg agttaagaga gtagatatat
    10201 gttgttgtgc gcgggctgta actaaaaatt aaaaggcagc ccgtacctat gagtccactg
    10261 tcaatggtgt aaatatcgat tcaaatcatt cattaataaa tgcactaaca aaacacaaaa
    10321 tagaataatc atcaagcgat gaagcaagac ttccacagcc actgagacag gtttcccacg
    10381 aattttctct ataaacgaat ataaaatgct gcctccatca aggccaggta taggaaataa
    10441 attgaccaaa gctacagcaa gactcaaagt agcaatgaat agtaaaaaaa cgataacgcc
    10501 ttgagttaaa gaagccacag aaacagaaaa aatagccaac ggtccaagta aaattgaaaa
    10561 aggaataacc ccgagaaaca attgcttcaa tatcattata aaaaagtaca tggtatggat
    10621 tatctcagca aacgcttttt gcattgaagc cagtagagat ggatagcgcg tcaaactgga
    10681 aactgctgac aaatcaggtt tgatccctag actctcgagc agagagccat cttttgaagt
    10741 aaattcaatc tgacttaaat caagattcaa ttgttttaat tgttgattcc cttgtcttaa
    10801 aacaatgtga acatcctttt gcccccaatg aattaccaat tgcataccta ccccttgcca
    10861 ggaatcagtt gggtaattct ctatagcaac ccattgatcg cctgcttgaa tacccgcctt
    10921 agcagcaagg ctatccagtt ttaccgattg aatttgaggt tgtttgtagc taatgccaat
    10981 ataaaaaacc aataccaagg ctatccatgc ggtaataagg ttggcaatcg ctcctgacaa
    11041 taaaatcagc acacgaatcc aaattggttt cttatcaaaa caataagcat tttcttgtgg
    11101 ctttactgga ctaatccgcg aattcagtaa ctgaacatac cctcccaaag gccacattgc
    11161 ccagatccag ttacaaccac tttgcgtttg ccattgaatt aatggttttc caaatcctat
    11221 ggaaatttta ctaattttga cctgaaacag gcgggctgcc agtgcatgcc ccagttcgtg
    11281 tatacctatc accagtatta acgtaagtat tatggctacc aatgccataa tcataatgga
    11341 ctccttatcc agaatatgag gcttattaaa aacacaccag aaagaataat ctatttgtgc
    11401 gtattatcaa ctaccagctg caacattgga atcccttttc ttccagccaa ttcatatcct
    11461 ttacgataaa cttcaaaaat tcgataaggt tgattcgtat cactatcaac caactccacc
    11521 tcatcgccct gttcatcaac taaagtgaca gtcaaatgaa tctcgcgaaa tccatcaacc
    11581 tggtaacgct ctttaaataa tttcagaacc ctctccaggc tctcaacagt ttcaatagca
    11641 ttatgttgac cttgcaggat aatgtccatt taaagctcct attgaaatga cacatgttgt
    11701 gcaatatgtg gctatacatg ttggcggcaa aactcaacag gactttagaa agtcactgtt
    11761 ctcattatta gatatgagcc cgtttgaaga tagagaaagt aaattgaatc ccttgccagc
    11821 aaattttccc aagtgggaca atcttgagga agaaggcggg taaaaaatat actgtaaaat
    11881 gattgcaaga aacagaaata caaggaacct tgtctttttc tctacaaatt ttacaatgaa
    11941 tcgtttacaa ttcgttaatt cccttaaaca aataaatcaa gattctatgg tatttgtagc
    12001 ttgcgggctt aatcataaaa ctgctccaat tcatgtgcgt gaaaaagtcg cactgcagcc
    12061 agccatgcaa gattctctat tgagcagtct gcttgattta cctgaagtca atgaagcggc
    12121 tattttatct acatgcaacc ggactgaaat ctactgtgat accaataccc ctgaggttct
    12181 tggaaactgg ttagctcatg aacaccaatt atcagaggaa ttattatctc aatttttgta
    12241 tatccatcag ggaaaagaag gaatcaaaca taccttgcgt gttgccagcg gtttggattc
    12301 catgatgata ggcgaaccac aaatccttgg ccaaatgaaa caggcgtatc aacatgcttg
    12361 tcgtcttggc acagtaaaaa cacaattacg gcctgtgttt gaatatattt tcagagcgtc
    12421 caaacgcatc agaactcgta gcggaattgg ggctaatccc gtatcgattg cctatgctgc
    12481 ggtacaactg atagggcaat tatttaaaaa ctatcattcc ttaagtgtct ttttaattgg
    12541 ttccggagaa acagcctctc tggttgctaa atacctgcat cagcatggtg tccatcgttt
    12601 tttgatagcg agccgcactc tggaaaatgc tcaaaaatta gctgaaactt tcggtggaaa
    12661 aacactgtcc attggtgata tcccgcaata tttacctctg gccgatgttg ttatttctgc
    12721 cacagcatgc ccactcccat tcatcaataa aagcctggtt gagcatgcct tagagcaaag
    12781 aaaccatgcc cccatgtttt tattggattt ggccgttcct cgtgatattg aaggaaatgt
    12841 caatgaattg gaacaagttc atctctataa tgtagatgat ttacaatcca tgatagaaaa
    12901 agggatggat gaaaggcgaa atgcggcatt acaagcagaa caattgatcg aaagcgaact
    12961 ggataactac attagatggc atcgctcgct tagagccaaa gacgtcattt gcgattaccg
    13021 caatcaaatg cacactttgg ctcaacagga attacaacgt gctttgaaaa aaatatccgc
    13081 tggtcaaaat cagcaagacg ttttaaatga attcagtatg cgcttggtca acaagctaac
    13141 tcataatccc accataggtt taagacaaat agcatgggat aacagagaag acttacttga
    13201 cctcgcgcga tatctctttg atacaacagc caatcaatca ttatatgaag aaatctcttg
    13261 agttaaaatt acagcaaatg ttggagcgat acgaggaagt aggccgctta ctgtccgaag
    13321 catccattat tgccgatcaa aaccaattta agtctttatc aaaagaatat gcccaacttg
    13381 aaccagtttc ccaatgttat gagtcgtatt tagaggcaaa aaataatttg gattctttaa
    13441 acgaattatt ggaaagtgat gataaagatt tagcgactat ggctgaagaa gagattgaca
    13501 ctgtcaagaa acaaattgag gaattggatg aacaattaca atggcatctc attcctaaag
    13561 atcctgatga tgaacgcaat atttatcttg aagtcagagc cggcacaggt ggagacgaag
    13621 cagctatatt tgccggagat ctctttcgaa tgtacagccg ttatgcagaa agccagggtt
    13681 ggcaaattga attaattagc gccagtcatg gtgaacatgg aggctacaag gaaataattg
    13741 ccaaaatcag tgggcaagcg gtttattcac aactcaaatt tgaatcagga gctcacagag
    13801 tacagagagt acctgaaact gaatcgcaag gtcgtgtcca tacttccgca tgcacggttg
    13861 ctatcatgcc ggaggtggat gaaattaatg acattcagat aaatcccgat gacttacgca
    13921 tcgatactta cagatcttcc ggagccggtg gacagcatgt taacaaaaca gattctgcca
    13981 ttcgtatcac acacattcct acaggagtgg ttgtagagtg ccaggatgag cgttcacagc
    14041 acaaaaaccg tgccaaagca atgtccttgt taaaaacaag attattagat gccgaagtaa
    14101 gcaaacaaaa acaagaacaa gctcaaacac gtaagtcact ggttggtaca ggtgaccgct
    14161 ccgagcgcat tagaacttat aactttcctc aaggccggct cactgatcac cgtatcaatc
    14221 tgactatcta ccagcttagt gacatcatgg aagggaattt atccctggtc attgacccgt
    14281 taaaacgcga ataccatgct gaactgctag ccgatttagg tcgacatgat tgatattcgt
    14341 tcattactgg aacaagctct gcaacaattc aaagtcaaaa accaggaaac caagttagag
    14401 gcagaacttt tactctgcca tgtgttaaac aaaaaccggg cctatctttt tgcccatccg
    14461 gatgctttgg tttctcctga acagatagaa acctatttgc agatgattaa acaacgtgct
    14521 gaaggtctac caatcgccta cattactgga cagcgggaat tttggtctct ttcgttaaaa
    14581 gtaacaccca atgttctcat tccaagacat gaaaccgagc gtctggtgga gcttgcactg
    14641 gagctaatac ctgacaagga gaatgtttct gtccttgatc tgggaacagg aagtggtgct
    14701 attgccttgg cgcttgcaaa agaaagaccc ctttggcata ttgacgcctg tgatttcagc
    14761 aaagaggcat tggagttagc ccgttataac gctaaaacac tcggcttaaa caatattaat
    14821 ttctgtcatt cctactggtt taataatctc cccctaaaac aataccacgc tattgtatct
    14881 aatccgccct acattgctga aaatgatcct catttaaaac atggcgatgt ccgctttgaa
    14941 ccaacaagtg cattagtcag tagtcaagat ggattagctg atctacaata tattattcag
    15001 cacagttatg aatatctttt gcctgatgga ctgctcttgg ttgaacatgg ttttgaacaa
    15061 aaaaatgaga taagcgctat acttaatcaa ttaggatata aaaacataca ctgttggcaa
    15121 gatcttcaag gacatgatag ggtaagtgga ggacaacgtc caagcaatcg gaataacaaa
    15181 cctttgtagt gattattttt ttgttgatga tactgacatt ttttggtata cgtagcgatt
    15241 ttgtataaaa ctcagtttca gatacagaat gaaatatagg gtctggcatg acaggacaat
    15301 taattgataa aaccaaagag agattataca acgtgaaaaa cgatgcaata ggaaatatgg
    15361 gaatagcgcc ctaccaagag actgagggcg aggaatatat gaatgaaaaa cagctggcac
    15421 atattgaaaa aatattgctc gcctggcgcc aatctttaat ggaagaagta gatcgtactg
    15481 tctcgcacat gaaagatgaa gcagccaact tcccagatcc ttccgacaga gcaagccagg
    15541 aagaagagtt tagcattgag cttagaaccc gcgacaggga acgaaaactc attaaaaaaa
    15601 tcgaagacgc tcttgagcga ttaagaaatg aagattttgg atattgtgaa gcctgcggaa
    15661 tcgaaattgg cctgaaacgt cttgaagcaa gacctactgc gaccttatgc atagattgta
    15721 aaactctatc tgaaatcaag gaacgccaaa atcagggtgc ttgattcttt cttgcctctg
    15781 aacattaaac aataaattcc cgccaggcgg gagtttatta ttacaagcaa tcgatgtttt
    15841 ttatgtattt taccaattat ggctacagat taaatcaaat caaaaaaggc tcggatcaac
    15901 actggctgtt tctgccaggt gggccggggc ttggttctga atatttagcc agtttttgcg
    15961 acaagctaac tttgcagggc actgtaagcc ttgttgattt tccaaaagat ggaaataata
    16021 gtgaaggcca gcttgatttt gtatcttggc aggaaggcct catttcattg ctgaaatctt
    16081 tgaacaagcc aatccttgtt acccatagtt tttcaggaat gttcacgctg aattttccgg
    16141 aaattgagaa tcatttaagc gggcttgttt taatgaatac aacaacaacc aacagtttct
    16201 ttcagcacgt taacgaaatg cgtcaaaaac ataatttgcc agatttagtc cctgctgcga
    16261 gccaatatca tttatatcca tccaacgaaa cctataaaaa attttgggat acttataaac
    16321 attattgttt cactcaggaa gagctaccaa tcggtgagca aatgatggaa ttatttgcct
    16381 acaacaatga atcctatcat tatgccatcg agcattttta taatgatttt cactataagt
    16441 ggcatcctca aaccattcca accatgacca ttgccagtga acttgattac atttgtccac
    16501 ctgcaatatt tattcaggat aagaattttc aatgtcaaaa tgtgattaat aaagtcattt
    16561 ataaagcagg ccattgtcca tggatactcc accttgaaca agttcaaaaa tgttttgatg
    16621 aattttctgg tatgatacaa gaaatcaata ttgagagaat tttataacaa gctaatatcc
    16681 caaaactgac tcaattttct gtaaatacct cactacagag cccttattac tttcaaggac
    16741 tgaactggcg tttgcaacca tagtgctttg agattcctta tcctgatata acttgataat
    16801 tgcatcaacc aactcattgg catgattcac tagcaaaatt gcctgagcct ctttaagctc
    16861 tctgcaaatt gatttaaagt tatgcacttg attcccgctt aaaacaggca cattcatcgc
    16921 gattggctcc aatacattat gaccaccaac cgggacaagg ctgccgccaa caaaagcaaa
    16981 atcactaatt tgataaaatc ccaaaagctc acccaagctg tcgagtataa caacttcatt
    17041 ttctctggaa atcgtatcca aattgctacg acacccagta ttaaatcctg cttgaacact
    17101 caactgatag accgtttgaa atcgttctgg atgacgcgga gcaattaata acactacccc
    17161 cggaatagcc tcctgcaaac gaggtagctg agataaaatc tgagactcct cgtcgtcatg
    17221 agtgcttgct gcaatgacgg taagtctatc ctctccccaa tgacttttta attccctgta
    17281 ttgactacta tcaacagagt caatctgcaa atcaaacttc atattcccta atacatggac
    17341 taaatcggca tttgcaccca aggcaatgaa tctctcagca tcctcattgc tttgagtcaa
    17401 aattcctgaa aattgattca aaaccggttt aaataaaaat ttaagcttta gatatccttg
    17461 caaggagcga tcagataagc gtccattcgc cagaaataat gccactcctg aagcatgagc
    17521 ctgcttaatt aaattaggcc acaattcagt ttccataatg atcccgacac gaggtttcgt
    17581 tcttttaaaa aatcgtttga gaacccaagg taaatcatag ggtaaatatt gatgcgctac
    17641 tttatgacca aatcgagatt ttactcgttc agaacctgta ggggtcattg tggtaactaa
    17701 cactgaccaa tgtttgttga gcattgcatc aattaaaggt gttgccgcga tcacttctcc
    17761 cagagaaacc gcatgaagcc atacatcaac tggtgcattt tcttgtattc ccagacaaaa
    17821 acgctctcct atcctttgcc tgtaagctgg caactttcta cctttccgcc acaggcgaaa
    17881 aagcaagtaa ggagtcagta agtacattaa aaaggaatac acaaaacgca taaacaggta
    17941 ccaaacacaa aaaggtaaag tatataccta aaacagttcc ttgtgcgata tgttacaatt
    18001 caattag
//
LOCUS       DACTJE010000054        18004 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.054, whole genome shotgun sequence.
ACCESSION   DACTJE010000054 DACTJE010000000
VERSION     DACTJE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18004
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.054"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..2103
                     /locus_tag="I9760_001421"
     CDS             <1..2103
                     /locus_tag="I9760_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HAT5043194.1"
                     /translation="NKQFDQINLQLSVQNLNLNNLNKAVETLGELSAQSDECIADIQK
                     KINGVDVLIKQATSTGNDKGADLVYLNNQKHQLAELQSQCRLFSIRAQEAIEAYKSAI
                     LQLRQEKTLARTLPLWQLASKIINSSDNTKWINHTKEKFLLQDLPVYLWFCIVAGSFF
                     ISILILSKLRQSNVIRRYVKLKKIRWSHITLLALFMMTGSFYGYISLFTAEEQLINSQ
                     FLLIKELFWFLCGTLFIVCLFKVKKIKAFFDWYAMDSEFFEILSIVIVAFYFLALSEQ
                     RLFDIWEVNKIIVELTRSIFLFLLLGTTAYFTHYFCRSHLHISFVKRHYNFIRYFIFA
                     LLSGCAILNINGFYTLAMQLTLSGIFTFFILYITILIGQSINKFYASLSYSQGKLNVI
                     RIFGYKKDQMLTEFYILKVTLKLVIVLLSVYLIGISWGFATDFIDTVYKKLLYGFTLA
                     NITIYPTRILFGIIIFCFLYLVSRAISTSISRHQQFENEEETQVAIASILTYVGFSIA
                     VISGLLVAGFNFTGLAIIAGALSVGIGLGLQSIVNNFVSGIILLIEKPIRPGDRINID
                     GVEGFVKKIRVRSTQILTPVNEDIIIPNSDLITRRVVNYMLTDNYWRVNCEVSVAFGT
                     NLNLVKDILLEIANKHDDVVKSGRNKPVVLFTSFADSALTFQLWCMIKDVNKKSTVKS
                     ELNFSIEEAFRKHDISIT"
     gene            2276..4420
                     /locus_tag="I9760_001422"
     CDS             2276..4420
                     /locus_tag="I9760_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT5043195.1"
                     /translation="MIKKIHRWLPLLVLIGLLVLFFSLHLDKYLSFNALRENHALLIA
                     WTQTHYFIAPLIFIVFYTTAVAISIPGAVLLTLTGGFLFGVFWGVLFVVISATLGATI
                     LFFAVRTALGDWFAQKASGWIERMRQGFQHNAFSYLVTLRLIPLFPFWAVNIVPALLN
                     IRAKTFITATFIGIIPGTTVYVMVGNGLSQIFAANQTPNLGIIFELQILGPLLALAVL
                     SLMPVLYQFSTRKHQNHHSFSDISRVINCDLAIIGGGAGGLSLASGCSQLGLKVVLVE
                     SGKMGGDCLNYGCIPSKSLLAAAKTFYYAKHATHFGVHTEAIKINFQQVMQHVHQIID
                     NISEHDSVQRFESLGVQVIKQVGKFLNPDTLQAGDSIIKAKRFVVATGSSPFIPPIPG
                     LDAVSYFTNETIFDLKEQPEHLIVIGGGPIGCELAQAFAMLGSKVTLLEGLNLLPKDD
                     PDCVAVLRTQMKSMSIVIHEQIEITQINSHPDTGISVCFEFQNTQFTITASHLLIATG
                     RRANVKPLDLEKAGVKLTSKGVEVNKYLQTSNKKIYALGDVTGLYQFTHMASYQASIV
                     LRNIVFKLPSKVDYRAIPWVTYTDPELAHVGIGVSDALKHPDAQIIEWPFVDNDRAQT
                     ERSLNGKIKIITDKKARILGVTIVGPHAGELILPWVMAIREKKNLRSFTDVIVPYPTL
                     SEISKRVAGSFYAPKLFSNKTRMLVRWLQKIG"
     gene            complement(4476..5186)
                     /locus_tag="I9760_001423"
     CDS             complement(4476..5186)
                     /locus_tag="I9760_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043196.1"
                     /translation="MIHLLINWVNLKLTMRASTINTILFLFLGVWGDLSHATKSIHTN
                     QVDSLFNIVKMSTVLSKDSLISPYNYLLTQPLMTTTLEKYYQRTSRIKVITAFTNPHN
                     NTYSRIIILYVDRNKKRNNVELAQKKNEIIPVELAAININFEELPEKLITDITHTNIP
                     FGKLLTQYHLKVFSKDRRYFSVICNNMLASLIHCKPNKKLYGRTNVLARVDNKKWVAH
                     VIEILSDEKEVKHRLRLN"
     gene            complement(5283..6008)
                     /locus_tag="I9760_001424"
     CDS             complement(5283..6008)
                     /locus_tag="I9760_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043197.1"
                     /translation="MENLEHGHSQKEIRDRLAQVPSQNYLRDWIYGGIDGVVTTFAIV
                     SGVVGGQLSSLVILILGFANLLADGFSMAASNYLGTKSEVDQFQRYKTIEEKHIDFIP
                     EGEKNEIKQIFQNKGLHGQTLDQVVEEITANRALWIKTMLQEEYGLPATLRFPLKSAL
                     YTFSAFLLFGIIPLIPYVLVMNNSFIWSCFFTAITFFVIGSIKSHWSTKSWLYSGFET
                     LIIGTVTASLSYGIGLFLHHYLT"
     regulatory      6101..6194
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6268..7212
                     /locus_tag="I9760_001425"
     CDS             6268..7212
                     /locus_tag="I9760_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT5043198.1"
                     /translation="MSSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEP
                     ADPSDVTEIVGLGTVDFGVKAMIHTVAAKAKGYPVTSIGTLLDEPPTGLIALKSSGIN
                     SFQDIVGKRVGYIGEFGKKIIDDLASLAGIDPTSYKTVRIGMNVTDAIYRDVIDTGIG
                     FINFQKVELEHLCGETVFLRIDQLAGLGCCCFCSIQFIVPEITLQQPELVKGFLRATQ
                     RGAAYTTEKPEEAYELLCQAKPQLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYT
                     KHLKIIDEHFDISQCYTNRFLPDTPYSDLKPIACCLEN"
     gene            7215..8285
                     /locus_tag="I9760_001426"
     CDS             7215..8285
                     /locus_tag="I9760_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT5043199.1"
                     /translation="MRAGIVGSGIMGRLLAFVLHNAGWQVSLFDRSNESMNCSTAAAG
                     LLTPFSELDKSILLISTLGQESIKNCWPRIIEDLPESVYFQQSGSLVLCHPGDQAEWI
                     HFSRRILSQLNPSNSVFQKLSHENLIALEPELTKFETAYYFPNEAHIDCQTLMLSLKN
                     YLDSVCIPHITNTPVLSLKPGEINTKEQRYHFDVVFDCRGLGAQNVFSDLRGIRGELI
                     WLHAPGVQLHRPIRLLHPRYSLYLVPRPGNFYLIGASEIEAEDFSPVSVRTALELLTA
                     VYYLHRNFDEARIIKTVTHCRPTLVNHLPRIKFADGLIAVNGLYRHGYLIAPALAEEI
                     LRGVTSNFRHLNYPELWENYCD"
     gene            8278..8481
                     /gene="thiS"
                     /locus_tag="I9760_001427"
     CDS             8278..8481
                     /gene="thiS"
                     /locus_tag="I9760_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAT5043200.1"
                     /translation="MINVYFNDKIYNIQPSLSLSAFLKQLNYTQQHFAVAINNHHVPH
                     KAYETTILQANDCIDILFPMQGG"
     gene            8484..9272
                     /locus_tag="I9760_001428"
     CDS             8484..9272
                     /locus_tag="I9760_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAT5043201.1"
                     /translation="MWNLAGKKLSSRLILGTACYPSLEHMQQAIHNSGTEVITISIKR
                     QTSAGLDGESFWQAVKKLDCHLLPNTAGCRNAEAAINTAEIARELFNTQWIKLEVIGD
                     DYNLQPEPFELIKAARILIDRGFEVFPYCTDDLVLCQKLVDAGCKILMPWGAPIGSGK
                     GLINPYALETLRYRFPDITLIIDAGIGKPSHAVQVMELGFDGVLLNTAVALANHPALM
                     ATAFRHAVIAGHQAFTGGMMSERNVAHPSTPLIDTPFWHQVNNL"
     gene            9269..10735
                     /gene="thiD"
                     /locus_tag="I9760_001429"
     CDS             9269..10735
                     /gene="thiD"
                     /locus_tag="I9760_001429"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAT5043202.1"
                     /translation="MKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQ
                     NAHSIIAVEEISRDQVAAQCRALELNLKPDAIKIGMLCSTPICEEIAYFLKGYEGFVV
                     LDPIITSSSGTNLFFPDLQQHKNNLIQLFPYITIITPNRIEAEIILNRSIYSYQDIIN
                     AASDLLSLGAKQVLLKGGHVKDNSFSQDYWTDGKESFWIANRRFPETNYRGTGCVLSS
                     ALTACLALGYSIKDAIVIAKMYVNRGIRQSIEIDKDASQLYHDGWPEDEADLPYLSPT
                     PFIKPVPSFKKYSMGFYPIVDSSHWLEMLLPLGIKCIQLRIKEASQERLEEEIKRSVH
                     LANQYNAALFINDHWELAIHYGAAGVHLGQEDLEKADVDRINRSGLFLGISTHCYYEV
                     ARAHALNPSYVACGPIYETTSKIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSD
                     VLKTKIDGVSVISAITKASAPLVAAKQFLTQMNESHNE"
     gene            10728..11855
                     /locus_tag="I9760_001430"
     CDS             10728..11855
                     /locus_tag="I9760_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAT5043203.1"
                     /translation="MNKSNLAMNKLTRYSQQIKMEEIGLDGQEKLKNSRVLCIGLGGL
                     GSPLLLYLAAAGVGVLGIVDDDIIELSNLHRQILYNHTHINKKKAVTAKKQLLAINPL
                     IQVESYSSRLTEENAAELITQYDIIADGTDNFYTRYLIHSICFELEKPYVYASASGFQ
                     GYCSIFHGKQDPCLHCIFPVSVSDINIPNCEGAGVIGTLPGMLGIIQATEIIKWILQI
                     GNPLKKRLLTINLLTMTFKNIIISRNPDCEFCIQNQSIRQLINPVNCSAQIEPCHHRI
                     TLDEFYQLFTNNKNIHLIDVRSTKEHQIQNIGGIVLPLDELPQRLHELNPNRLIILYC
                     QSGTRSKMALDILLKAGFASVKYIENGLKGVTDNYNKDMPQ"
     gene            complement(11884..12717)
                     /locus_tag="I9760_001431"
     CDS             complement(11884..12717)
                     /locus_tag="I9760_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HAT5043204.1"
                     /translation="MIRSILVLFFLFIANAGYAWNAIGHQLVAQIAYDNLTPQSRRMC
                     DLYSHSKSKTSSNVNFVKSASWLDSIRAHDVHWFDALHYIDIPFSMDETELPVLTDIN
                     ALWGINQAIAVLSSKKASIADKKLSLRILVHLVGDIHQPLHTVTKISKKLPKGDLGGN
                     LFQLAKNPIGNNLHQYWDNGGGILIGQDKFFQIKNKARQLEKKWSCQSASKEKNPQQW
                     INASHQLALTKVYKVSAHQVPGKQYQLNTQNITEKQILLAGCRLAYLLNNIAEGKNKL
                     I"
     gene            complement(12720..13979)
                     /gene="tolB"
                     /locus_tag="I9760_001432"
     CDS             complement(12720..13979)
                     /gene="tolB"
                     /locus_tag="I9760_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HAT5043205.1"
                     /translation="MFNRIISLFLLLFTGQVIALDLELTQGINSALPIAINSFGSNAA
                     AQEIGNVIENDLTISGQFKIISGPQGANSQSSVSTLRQLGADSVVTGRVNQVGNRIEV
                     SFTLADAVANGNILLTKTFQINANQVRALAHHISDEVYQKLTGERGIFSTRIAYISVQ
                     RNGGRSRYSLEVADADGHNPQSLLVSSEPIMSPSWSPNGKSISYVSFEKKKAEIFTVS
                     VETGQRRLITSFPGINGAPAWSPDGQHLAVVLSKSGTPKIYDVDLSSGSMKQLTFGNS
                     IDTEPRYSPDGRSLLFTSGRGGSPQVYRLSLADGQISRVTFEGNYNARASYTPDMKHI
                     VMLHREDRQFNIGVQNTGGGPISNLTFSGLDESPSVSPNSRLVLYATRYQDRGVLGIV
                     SIDGRIRMRLPAREGDVQEPAWSPYLS"
     gene            complement(13985..14983)
                     /gene="tolA"
                     /locus_tag="I9760_001433"
     CDS             complement(13985..14983)
                     /gene="tolA"
                     /locus_tag="I9760_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAT5043206.1"
                     /translation="MISDLTYRKAFLFAVTLHLFLLVLLFTDNSSKKPVLTIESKNSP
                     GMMQPTASQQEVVKAVSVDNKEIMKTVNRLKQEREQQKKAELNRQKELLRQAEAAKQQ
                     RIKEQQEIARLKEEANKIAIARKKQVEEEKKRLKELAEQKALEAKRIEELKKQNEKLA
                     KQRELEAKKLAELNKKKQEEKEKAEKLKAEQAKAEKLKAEKARAEQEKAEMERKRQAE
                     AAAAQQAAQNAERQARIAGEVDKYKALIVNAIGRNWILPENVDSSLSSQFRIRLAPDG
                     TVLEVSLIRSSGDPLLDRSAQTAIYKASPLPVPQDVETFNLFRDISLTVRPEQVRG"
     gene            complement(14980..15435)
                     /gene="tolR"
                     /locus_tag="I9760_001434"
     CDS             complement(14980..15435)
                     /gene="tolR"
                     /locus_tag="I9760_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HAT5043207.1"
                     /translation="MNRSKTNRERPISEINVVPYIDVMLVLLVIFMITAPMLTQGVTV
                     DLPKAASQTLQAADREPIIVSVNQLGSYFLNISSTPAEPIEAQALLVRVAAELELAKQ
                     SGQKLNVLVKGDQGVAYGKVVQAMALLKQAGAEQVGLLTDSQTGDEGQA"
     gene            complement(15444..16118)
                     /gene="tolQ"
                     /locus_tag="I9760_001435"
     CDS             complement(15444..16118)
                     /gene="tolQ"
                     /locus_tag="I9760_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HAT5043208.1"
                     /translation="MANQANVLMYFMQAGLVVKSVMLILLIASIISWTLIFQRAWFFN
                     RRKQMTETFTRRFWDCGDLSKLYADVDSNSDERQGVAAIFHAGFKEFVRCRKQGKVSI
                     EPIQRVMQISHAKEAEKLEQHLPFFASVGSISPYVGLFGTVWGIMTSFQALGHAQQAT
                     IAMVAPGISEALVATALGLFTAIPAVIAYNRYTTRANTLLNKYDLFQEELISLIEQQT
                     SGSPRG"
     gene            complement(16146..16541)
                     /gene="ybgC"
                     /locus_tag="I9760_001436"
     CDS             complement(16146..16541)
                     /gene="ybgC"
                     /locus_tag="I9760_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAT5043209.1"
                     /translation="MNLISNHQHNIRVYAEDVDYMGIVYHANYLCYLERARTELLRQN
                     NLILSEFEKSDILFAIHELTINYVFPARLDDQLTIKTEIKEFRACSFLFNQEISNQHK
                     QLICRASVQVVCVGSNLKPKRLPNIMRNE"
     gene            complement(16545..17555)
                     /gene="ruvB"
                     /locus_tag="I9760_001437"
     CDS             complement(16545..17555)
                     /gene="ruvB"
                     /locus_tag="I9760_001437"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAT5043210.1"
                     /translation="MLESDRLISSQSIVSEDAMDRAIRPLSLSEYVGQDSVSSQMQIF
                     INAARKRNDPLDHVLIFGPPGLGKTTLANIIAHEMGVNIRQTSGPVIERAGDIAAILT
                     NLQQNDVLFIDEIHRLSPVIEEILYPAMEDYKLDIMIGEGPAARSIKLELPPFTLIGA
                     TTRAGLLTSPLRDRFGIVQRLEYYSVDSLTQIVARSAHLLGVPTKPEGAREIALRSRG
                     TPRIANRLLRRVRDYSEVKGNGIITVDMAQQALEMLEVDQHGFDLMDRKLLLAVIEHF
                     NGGPVGIDSIAAAIGEEKGTIEDVLEPFLIQQGFLMRTPRGRIATSKAYQHFGFSAIE
                     QE"
     gene            complement(17702..>18004)
                     /locus_tag="I9760_001438"
     CDS             complement(17702..>18004)
                     /locus_tag="I9760_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HAT5043211.1"
                     /translation="YFRTNNLYADSEMQYMDEQFSTPHNSPESDIMGLEFEKQIEAAM
                     SCLSEELRLCYGLHIFDGQTYQDIAKEMHCPIGTVRSRIFRARKQLMDIVSENHTL"
BASE COUNT         5337 a         3476 c         3369 g         5822 t
ORIGIN      
        1 aataaacaat ttgatcaaat caatctccag ttgtcagtac aaaatcttaa tttaaataac
       61 ctgaataagg cagtagaaac tctcggtgaa ctgtccgcgc aatccgatga atgtatagct
      121 gatatacaga aaaaaataaa tggagtggat gtattaatca aacaagctac ctctacaggg
      181 aatgataaag gagctgattt agtctatctg aataatcaaa agcatcagtt agctgagtta
      241 caatctcaat gtcgattatt ctcaattcga gctcaggaag caatagaagc ttataaaagt
      301 gctattttgc aattaaggca agaaaagact ttggcgcgta ccttaccatt atggcaatta
      361 gcaagtaaaa ttattaattc atctgataat accaaatgga taaatcatac taaagagaaa
      421 tttttacttc aggatttgcc tgtatatcta tggttttgta ttgttgcagg aagctttttt
      481 atctctatcc tgatcttgtc aaaattacgg caatccaatg ttatacgccg ttatgtgaaa
      541 ttaaaaaaaa ttcgctggtc tcatattaca cttttggctt tgtttatgat gacgggttca
      601 ttttatggct acatttccct atttactgct gaagagcagc taatcaattc tcaattcctt
      661 ttaataaagg agcttttttg gtttctttgc ggcacattat tcattgtttg tttattcaaa
      721 gtcaaaaaaa tcaaagcttt ttttgattgg tatgctatgg atagtgaatt ttttgaaata
      781 ctaagcattg tgattgttgc attttatttc ctcgcattat cagaacagag attatttgat
      841 atttgggaag tcaataagat tattgtggag ttaactcgct ccatctttct ttttttattg
      901 cttggtacaa ccgcttattt tactcactat ttctgccgtt ctcatctgca tatttctttt
      961 gtaaaaagac attataattt tattcggtac tttatttttg cactgctttc agggtgcgca
     1021 attcttaata ttaatggctt ttatacttta gccatgcagc ttactctatc tgggattttc
     1081 acatttttta tcttgtatat cactatttta ataggacaga gtattaataa attttatgct
     1141 tcattaagtt actcacaagg taaattgaat gttatacgaa tattcggata taaaaaggat
     1201 cagatgctta ctgagtttta tattcttaaa gttaccttaa aacttgttat tgtactccta
     1261 agtgtctatc ttatagggat atcctggggt tttgctactg attttattga tactgtttat
     1321 aagaaattat tgtatggctt tactctggca aatattacaa tttatccaac gcgcattctc
     1381 ttcggaatca taattttttg ttttttatat ttggtgtctc gcgccatttc aacttcaatc
     1441 agtcgtcacc aacagttcga aaacgaagaa gaaactcagg tagctattgc ttccattttg
     1501 acttatgttg gattcagtat tgctgttatt tcaggcttac tcgtcgcggg ctttaatttt
     1561 actggacttg ccattatcgc tggagcactt tctgtaggaa ttggtcttgg actgcaaagc
     1621 atagttaata attttgtatc aggtataatt ttgcttattg agaaaccaat aagacctgga
     1681 gatcgaatta atatcgatgg tgttgaagga tttgtaaaaa aaatcagagt cagatcaaca
     1741 caaattctca ctccggtaaa tgaagatatt attattccaa actctgattt aattacacgc
     1801 cgtgttgtca attatatgct gacagataat tattggcgtg ttaattgtga ggttagcgtt
     1861 gctttcggga caaacctgaa tcttgttaaa gacatccttt tggagatcgc taataagcat
     1921 gatgatgttg taaaaagtgg cagaaacaaa cctgttgtgt tatttaccag ttttgccgac
     1981 agtgcattaa cttttcaact ctggtgtatg attaaagatg taaataaaaa atcgacagtc
     2041 aaaagtgaat taaatttctc tattgaggag gcttttcgca agcatgacat ttcaataaca
     2101 tagtcttaat gtgattttca tatttgaatt ggagattaaa tactttccct actctggaaa
     2161 ataatgcttg gtatcattaa accctatcga taattaagat atgacctcaa agtaaattcc
     2221 tgttaggatc ttcatataat ttatatcagc ttatcgggtt aaaggaataa catgaatgat
     2281 taaaaaaata catcgttggt tgcctttgct tgtattaatc gggctattgg tgcttttctt
     2341 cagcctacac cttgataaat acttaagttt taatgcgtta agagaaaatc atgctttact
     2401 cattgcgtgg acccaaactc attattttat tgcgccactg atttttattg ttttttatac
     2461 cactgctgta gccatctcta ttccaggagc agttcttctt actttaactg gaggattttt
     2521 attcggagta ttctggggcg tgttatttgt agtcatcagc gctacgcttg gggccaccat
     2581 actgtttttt gccgttcgca ctgctttggg cgactggttt gcccaaaagg catctggatg
     2641 gatagagcgt atgcgtcagg gatttcagca taatgctttt tcttatttag ttacactgcg
     2701 attaattcca ttatttccct tttgggcagt taatattgta ccggctcttt taaacattcg
     2761 agcaaaaaca ttcattacag cgacttttat tggcatcatt ccaggaacaa cagtatatgt
     2821 aatggttgga aatggtttaa gccaaatatt tgcagccaat cagacgccta atcttgggat
     2881 catatttgaa ctgcaaatat taggtccact gctcgcgttg gctgttctct ctctaatgcc
     2941 agttctttat caattctcaa ctcgcaagca tcagaatcat cacagtttct cagatattag
     3001 tcgagtaata aattgtgatt tagccattat aggtggtggt gccggaggtt tgagtttagc
     3061 ttccggttgt tcacagttag gactcaaagt tgttttggtg gaatcaggga aaatgggagg
     3121 agactgttta aattacggtt gtatcccatc caaatcctta cttgctgcag ccaagacgtt
     3181 ttattatgca aaacatgcaa cacattttgg agtacatact gaagcaataa aaattaattt
     3241 tcaacaagta atgcaacatg tgcatcaaat catagacaac atttctgaac acgattctgt
     3301 ccaacgtttt gagtcgctag gagtgcaggt cattaaacaa gttgggaaat ttttaaaccc
     3361 tgatacctta caagctggcg atagcattat taaagcgaaa cgcttcgtgg ttgcaacagg
     3421 ttcatcacca tttatcccac ccattcccgg attggatgca gtctcctact tcaccaatga
     3481 aacgattttt gatttaaagg aacaacctga gcacttgatt gtcatcggtg gtggccctat
     3541 tggttgtgaa cttgctcaag cgttcgccat gctcggttct aaagttactc tgctcgaagg
     3601 attgaatctt cttcccaagg acgatcctga ttgtgttgct gtcctacgca cacagatgaa
     3661 atcgatgagt attgtcattc atgaacaaat cgagattacg caaataaact ctcatcctga
     3721 tacgggtatc tccgtatgtt ttgaatttca aaacacacaa ttcacaatta cagccagtca
     3781 tctgctcatt gcaactgggc gtcgggcgaa tgtaaagcct cttgatttgg aaaaagcagg
     3841 ggttaaactc accagcaaag gtgtcgaagt aaataaatac ctacaaacca gtaataaaaa
     3901 aatctatgct ctgggtgatg taacagggct ttaccagttt actcatatgg cgagttatca
     3961 ggccagtata gtgcttcgta atattgtttt taaactaccg tctaaagtgg attatagagc
     4021 gattccatgg gtcacttata ctgatcccga attagctcat gttggaatag gagtcagtga
     4081 tgccttgaag caccctgatg cccaaataat cgaatggcca tttgtcgata atgatcgagc
     4141 acaaactgaa cgctcgttaa atggaaaaat aaaaatcatc acggacaaga aagcaaggat
     4201 ccttggagtc accatagttg gcccacatgc cggagaactc attttacctt gggttatggc
     4261 gattcgtgag aaaaaaaacc tccgaagttt tactgatgta atcgttcctt accctactct
     4321 gagtgaaatc agcaaacgag ttgctggtag tttttatgca cctaaattat tttccaataa
     4381 aacacgaatg ttagttcgct ggttgcaaaa aattgggtaa atagattttt aactaactat
     4441 atgttttttg gctgaaagaa aaaattacaa gctatttaat tcaatcttaa acgatgtttg
     4501 acttcttttt catcagataa aatttcaata acatgggcaa cccatttttt attgtctact
     4561 ctagcaagca cattggtacg gccatataat tttttatttg gtttgcaatg gattagtgaa
     4621 gccaacatgt tgttgcaaat aaccgagaaa tagcgacggt ctttactgaa aactttcaga
     4681 tgatattgag ttaataattt tccaaaaggg atattggtgt gagttatatc agtaataagt
     4741 ttctccggca gttcttcaaa attaatgtta atagctgcta attcaactgg aatgatttca
     4801 tttttctttt gggcgagctc tacattattt cttttcttat ttcggtctac atacaggatg
     4861 ataattcgag aataggtatt attatgcgga tttgtaaagg cagtaattac tttaatcctg
     4921 gaggtgcgct gatagtactt ttccaaagtg gtcgtcatta atggctgtgt aagtaagtaa
     4981 ttgtaaggag agattagact gtctttgctt aatacagtgc tcattttaac aatattgaac
     5041 agactatcta cctgatttgt gtggattgat ttagtcgcat gtgataaatc cccccaaaca
     5101 cctaaaaaaa gaaataaaat agtattgatg gtacttgctc gcatggtgag ttttaaattc
     5161 acccagttga ttaataaatg aatcatttct agcatgacct tatataatta actacaaaat
     5221 tcctatctaa caaaagcatc cattgggatt ataggatgaa agcagcagat ccatgccgta
     5281 ttttaagtca gataatgatg taaaaatagt ccaataccat aagatagaga ggctgttaca
     5341 gtgccaatta ttagtgtctc aaaaccggaa tataaccagg atttggttga ccagtggctt
     5401 ttgattgagc cgatgacaaa aaatgtaatt gcagtgaaaa aacaagacca gatgaacgaa
     5461 ttgttcatga ccagcacgta aggaattaat ggaataattc caaaaagtag aaacgcacta
     5521 aaagtataaa gcgcagattt aagcgggaat cgaagtgtag caggcaaccc atattcttct
     5581 tgtaacatgg tttttatcca taatgctctg ttcgctgtaa tttcctcaac aacttgatcc
     5641 aatgtttggc catgcaaacc tttattttga aaaatttgtt ttatttcgtt tttttcaccc
     5701 tctggaataa aatcaatatg tttttcttca attgttttat aacgctgaaa ttgatcgacc
     5761 tcagatttgg tacccagata attacttgcc gccattgaaa acccatcagc aagcaagttg
     5821 gcaaaaccta aaattagaat aactaaagaa gatagttgtc cacctacaac accagaaaca
     5881 atggcaaaag tagtgacaac accatcaatt cctccatata tccaatctcg caagtaattt
     5941 tgagatggga cctgtgctaa tcgatctctg atttcttttt gcgaatgtcc atgctctaaa
     6001 ttttccatag cagatccttt ctccatatgt gttatttatt ctgccaatat tatcgctagt
     6061 ttgacaaagc atataataaa atttagtata tttgagtttg ttagatggcg cggggtgtcg
     6121 ggaaatccgg ctgagagtta cccgttgaac ttgatctgaa tcatatcagc gtaaggacgc
     6181 cttcaaaaaa accatggctt ttttaaagca tggttccctt ttgccatctc cataatattt
     6241 aaaataataa cataaggagt tatggcgatg tcatcactaa aatccagggt taccctactg
     6301 cttaattggt acaccaaccc ttatcatacc cccatacttg tggcacaaca attgggattt
     6361 tattcggaag aagatatcaa gctcgccatt ctggagcctg cagatcccag tgatgtgacc
     6421 gaaattgttg gcctgggaac agtagatttc ggtgttaaag caatgattca tactgttgca
     6481 gccaaagcaa aaggttatcc agttacttcc ataggaacgc ttcttgatga gccccctacc
     6541 ggacttattg ctttaaaatc aagtggcatc aattctttcc aggatattgt tgggaaacga
     6601 gtaggttata tcggcgaatt cggcaaaaaa attattgatg atttggccag cctcgcaggt
     6661 attgatccga cgagctacaa aacagtacgt attggcatga atgtcacaga tgccatttat
     6721 cgggatgtta tcgacacggg tattggtttt attaattttc aaaaagttga actggagcat
     6781 ctttgcggag aaactgtatt tttacgaatc gatcaattag ctggtttagg ctgttgttgt
     6841 ttctgctcaa tacaatttat tgttcctgaa ataacattac agcaacccga attagtcaaa
     6901 ggatttctca gagcaacaca gcgcggtgca gcctacacga cagaaaaacc tgaagaagcc
     6961 tatgagttat tatgtcaggc taaaccacag ttgcggactc ctttatatca aaaaattttt
     7021 acaagaacat taccattttt ttcacgtaca ttaattaatg ttgacagaga ctgggataag
     7081 gttggacgct ataccaagca cctaaaaatt attgatgaac attttgatat ctcgcaatgc
     7141 tatacgaatc gttttttgcc agatacacct tattctgatt tgaaaccaat cgcctgttgt
     7201 cttgaaaatt aattatgcga gcaggcattg taggctcagg gatcatgggg cgtctgctgg
     7261 cttttgtatt acataatgcg ggctggcagg tatccctttt tgatcgctct aatgaaagca
     7321 tgaattgcag tactgctgct gcaggtctat taacaccgtt ttctgaattg gataaatcaa
     7381 ttctattgat ttccaccttg ggtcaagaat ctattaagaa ctgttggcca cgtattattg
     7441 aggatttgcc tgaatctgtt tattttcaac aatcgggtag tctggtgcta tgccacccag
     7501 gcgatcaggc ggaatggatt cactttagtc ggcgtattct gagtcaactc aatccgagta
     7561 attctgtttt tcaaaaatta tcccatgaaa atctcattgc gcttgagcct gagctgacaa
     7621 agtttgaaac tgcttattat tttccaaatg aagcgcatat tgattgccaa acactaatgt
     7681 tgtctttaaa aaattatctc gactctgttt gtatacctca cattaccaat actcctgtct
     7741 tatcgcttaa acctggagag atcaatacaa aagaacaaag atatcatttt gatgtcgttt
     7801 tcgattgtcg tggtttaggt gcgcaaaatg tattttctga tttacgaggt attagaggtg
     7861 aactcatttg gcttcatgcg ccaggagttc aactacatcg ccctattcgt cttctgcatc
     7921 caaggtacag tctatatctt gtacccagac ctggaaattt ttacctcata ggcgctagcg
     7981 aaattgaggc agaagatttt agtccggttt cagtacgtac ggctttggaa ttattgaccg
     8041 ctgtgtatta tttacatagg aactttgatg aagcacgcat tataaaaaca gtgacccatt
     8101 gccgtccaac cttagtaaat catttaccac gtattaaatt tgccgacggg ctaatagcag
     8161 ttaacggtct ttatcgtcat ggttatttaa tagccccagc cttggcagaa gaaattttac
     8221 ggggtgtgac atccaatttt cgtcatctga attatccaga actttgggag aattattgtg
     8281 attaatgtct atttcaacga taaaatttac aacatccaac cctcattatc attaagcgca
     8341 tttttaaagc agcttaatta tactcaacag cattttgctg tcgctataaa taatcatcat
     8401 gtaccacata aagcttatga aactacaatt ttgcaagcca atgactgcat tgatatcctc
     8461 tttcccatgc aaggaggtta atgatgtgga atttggcagg aaaaaaatta agtagccgtt
     8521 tgatcttagg tactgcgtgt tatccttcgt tagagcatat gcagcaggca attcataatt
     8581 cgggtacaga agttattaca atatcaataa aacgccagac ttctgcagga ttggatggag
     8641 agtccttttg gcaagcggta aaaaaactag attgtcatct tttgcccaat acggctggtt
     8701 gccgtaatgc ggaggcagcg atcaatacag ctgaaattgc gagggagtta tttaatactc
     8761 aatggataaa actggaagtg ataggcgatg attataattt acagcccgaa ccatttgagt
     8821 taatcaaggc agccagaatt ttgattgaca gaggatttga agtttttcct tattgcacag
     8881 atgatctcgt tttatgtcaa aaactggttg atgctggttg taaaatttta atgccctggg
     8941 gagctcctat aggctcaggt aagggtttaa ttaaccccta tgccttggaa actttgcgtt
     9001 atcgatttcc tgatatcaca ttaatcattg atgccgggat tggtaaacca tctcatgctg
     9061 tacaggtgat ggaattgggt tttgatggcg tgcttctaaa tactgctgta gctcttgcta
     9121 atcatcctgc tctcatggca acagcattcc gccatgctgt tattgcaggt catcaggcat
     9181 ttacaggagg aatgatgtct gagagaaatg tagctcaccc aagtactcca ttaatcgata
     9241 ctccattttg gcatcaggta aacaatttat gaaaaaaccg attgtttgga caattgctgg
     9301 tgttgactca tccggattag ctggtgtcca tgctgacatg gaaactttta gtcgcctgaa
     9361 tgtcagagct tgttcagtca tcaccgcagt aactgcacaa aatgctcact caattatagc
     9421 agtagaagag atatccagag atcaggttgc tgctcagtgc cgtgccttgg aattaaattt
     9481 aaaaccggat gccattaaaa ttggtatgtt atgttctaca ccaatctgtg aagaaatagc
     9541 ttatttctta aagggttatg aaggatttgt tgttcttgat ccgataatta cctcctcctc
     9601 gggaactaac ctgttttttc cagatttaca acagcataaa aataatttaa ttcaattgtt
     9661 tccgtacatt actattatta ctcctaatcg gatagaagca gaaattattt taaatcggag
     9721 catttattct tatcaggata taatcaatgc tgcttcagat ctgttatcac ttggagcaaa
     9781 gcaggtatta ctgaaaggag gacatgtaaa agataactca ttcagtcaag attattggac
     9841 tgatggaaaa gagtcgtttt ggattgcaaa tcgtcgtttt cctgaaacaa attaccgtgg
     9901 gacaggctgt gttctatctt cagcactcac agcttgtctt gctctaggct actcaataaa
     9961 agatgctatt gttattgcta aaatgtatgt caatcgtggc atacgccaat caatagaaat
    10021 tgataaagac gcctcgcaac tttatcatga tggttggcca gaagatgagg ccgatctccc
    10081 ctatttatca ccaacaccgt tcattaaacc agttccttca tttaaaaaat attctatggg
    10141 tttttatccc attgtggata gtagccactg gttagaaatg cttttgccat tgggaattaa
    10201 gtgcattcag ttacgaataa aagaagcctc tcaagagcgg ttggaagaag agataaaacg
    10261 gagtgtccat ttggcaaatc agtataatgc tgcattattt attaatgatc actgggaact
    10321 tgctattcat tatggtgctg caggagttca ccttggccaa gaggatctgg aaaaagctga
    10381 tgtcgataga attaatcgat caggtttatt cttaggtata agtacgcatt gctattacga
    10441 agttgctcgg gctcatgctc ttaacccttc ctacgtcgct tgcggcccca tatatgaaac
    10501 aaccagcaaa ataatgccat ttcaagcaca aggaattgca cgacttgaac gttggcgaaa
    10561 aacgcttcgt tatccattag ttgccattgg aggtataact ttaaagaacc tttcagatgt
    10621 cttaaaaaca aaaattgatg gtgtgtcagt gatctctgcc attacaaaag catcagcacc
    10681 tttagttgca gccaaacaat ttttaactca aatgaatgaa tcacataatg aataaatcaa
    10741 atttggcaat gaacaaatta acccgctatt ctcaacaaat aaagatggaa gaaattggat
    10801 tagacggaca ggagaaactt aagaattctc gagtattatg cattgggctt ggcggtttgg
    10861 gttcgcctct cttactttac cttgctgcag caggtgtcgg agtgttaggg attgtcgatg
    10921 atgacatcat tgagctaagt aatttgcata ggcagattct ttataatcat acccatatta
    10981 ataaaaaaaa ggctgttact gcaaaaaagc aacttctggc tattaatccc ctgattcaag
    11041 ttgaatctta ctccagtaga ttaacagaag aaaatgcggc tgagctaatt acgcagtatg
    11101 acatcatcgc agatggaacc gataattttt atacgcgata tttgattcac agcatttgct
    11161 ttgaactgga aaaaccttat gtgtatgcta gtgcctcagg tttccaaggg tattgctcta
    11221 tattccatgg gaaacaagat ccctgcctcc attgtatctt cccggtttct gtaagcgata
    11281 tcaatattcc caattgcgag ggagccgggg taattggtac tttacccggg atgctaggta
    11341 ttattcaggc aacagaaata atcaaatgga tactacaaat tggcaatcca ttaaaaaagc
    11401 gtttgcttac tattaacctg ttaacaatga cctttaagaa catcattatt tcaagaaatc
    11461 cggattgtga attctgcatt cagaatcagt caattagaca attaattaat cctgttaatt
    11521 gttcagctca aattgagcca tgccatcata gaataactct tgatgaattt tatcaattat
    11581 ttacgaataa taaaaacatt catctaattg atgttcgtag tacaaaggaa catcaaatac
    11641 aaaatatagg gggtattgtg cttcctttag atgagctgcc tcaacgctta catgaactaa
    11701 accctaatcg gcttattatt ctgtactgcc agtcgggaac gcgaagcaag atggcgttag
    11761 atatacttct caaggctggc tttgcttctg ttaaatatat agaaaatggt ttaaagggag
    11821 taactgacaa ttacaataaa gacatgcccc aataattcac ccctcctaaa aaggaagctg
    11881 aaattaaata agtttatttt taccttcggc aatattattt agcagataag cgagtcgaca
    11941 cccagccaga agtatctgtt tttctgtaat attttgagta tttaactgat attgcttgcc
    12001 gggcacctga tgagctgaaa ccttataaac tttagtcaat gccaattgat gtgatgcatt
    12061 aatccattgt tgcggatttt tttccttact ggctgattgg caagaccatt tcttttccaa
    12121 ttgcctcgcc ttgtttttaa tctgaaaaaa tttgtcttga ccaatcagaa taccgccacc
    12181 attatcccaa tattgatgca aattattccc aataggattt ttagctaact gaaataaatt
    12241 accacctaaa tcacctttag gtaatttttt acttatcttt gtaacggtat gcaaaggctg
    12301 atgaatatct ccaaccaaat gcaccaatat gcgtaagctc aattttttat cagcaatact
    12361 ggcttttttg gaagacagca cagcaattgc ctgattaatt ccccagagtg cattgatgtc
    12421 tgtcaagact ggtaattctg tctcatccat agaaaaagga atgtcaatgt aatgtaaagc
    12481 atcaaaccaa tgaacatcat gtgctcttat ggaatccagc catgaagctg attttacaaa
    12541 attaacatta gacgacgtct tcgattttga atgggaatat aaatcacaca ttctcctgga
    12601 ttgaggtgtc aaattatcat atgcgatctg ggctaccagt tggtgcccta tcgcgttcca
    12661 tgcataacca gcattagcaa taaataaaaa aaataatacc aatatgcttc ttatcattct
    12721 taactcaggt aaggggacca agcgggctct tgaacatctc cttcacgagc aggaagtctc
    12781 attctaattc tcccgtcaat tgaaacaatg cctaaaaccc ctctgtcctg ataacgggta
    12841 gcataaagca ctaaacgact atttggagag actgatggtg attcatcaag gccagaaaag
    12901 gttaaattgg aaatcgggcc accacctgta ttttgtacac caatattaaa ttgtctatcc
    12961 tccctgtgca gcatgacgat atgcttcata tctggagtat aggaggcacg ggcattataa
    13021 ttgccttcga aagtgactcg acttatttgg ccatcggcca atgataagcg atagacttgc
    13081 ggtgacccgc ccctccctga agtgaacagc aaactgcgac catcaggaga ataacgtggt
    13141 tccgtatcga tagaattgcc aaaagtaagc tgtttcatag aaccgctgct caaatcaaca
    13201 tcgtaaattt tgggtgtacc gcttttggat aatacaactg ccaaatgctg accgtcaggt
    13261 gaccatgcgg gcgctccatt aattcctggg aagctcgtaa ttaagcgacg ctgtcctgtt
    13321 tcaacagaga cggtaaaaat ttcagctttt ttcttttcaa aagaaacata ggatatcgac
    13381 tttccattgg gtgaccatga aggtgacata atgggctctg aagagacgag taagctttga
    13441 ggattgtgtc cgtcagcatc agcaacctcc agggagtagc gagaccttcc tccatttcgt
    13501 tgtaccgaaa tataagctat cctggtggag aatatccccc gttctccagt cagtttttgg
    13561 tatacctcat cactaatatg atgagccaaa gctcttacct gattggcatt gatctgaaag
    13621 gtttttgtca acaaaatatt gccattcgct acggcatcag ccaaagtaaa gctcacttca
    13681 atccggttac caacctgatt aactcggcca gtgactacac tgtcagcgcc aagttgacgt
    13741 aatgtactca cggaagattg agaattcgcg ccttgtggtc cagagataat tttaaattgt
    13801 cctgatatag tcaaatcatt ctctatgaca tttcctattt cctgagccgc cgcgtttgag
    13861 ccaaaagaat tgatggctat gggcagggct gaattaattc cttgagttag ttccaaatca
    13921 agagcaatca cttgaccggt aaataacagc aagaaaagtg aaataattcg attgaacacg
    13981 agctttaccc cctaacttgt tctggacgaa cggttaaact gatatcgcga aacaaattaa
    14041 atgtttcaac gtcttgaggc acgggaagtg gtgaagcctt gtatattgct gtttgtgctg
    14101 acctatctaa caaaggatca ccactgctgc ggattagaga cacttccagt acagttccat
    14161 caggtgcaag gcgtatcctg aattggctcg acagagaact gtcgacattt tcaggtaaaa
    14221 tccagttgcg tccgatcgca ttgacaataa gtgccttgta tttatcaact tcaccagcta
    14281 tccgtgcttg tctttcagca ttttgggctg cctgttgggc tgcagccgct tctgcttgtc
    14341 tttttctttc catctctgct ttttcttgct cagcacgggc tttttccgct ttcagctttt
    14401 cagccttcgc ctgttcagct ttaagtttct cggctttctc tttttcttct tgttttttct
    14461 tattgagttc agcaagtttt ttagcttcca attcccgttg tttggccaac ttttcgtttt
    14521 gtttctttaa ctcttcaatg cgtttcgctt ctaatgcttt ttgctcagct aattctttta
    14581 accgtttttt ttcttcttca acttgttttt ttctggcaat tgcaatttta ttagcttctt
    14641 ctttcaatct tgcaatttct tgttgctctt tgattctttg ctgttttgca gcctcagctt
    14701 gccttaataa ttccttttgt cgattaagtt cagctttctt ctgttgttca cgttcttgtt
    14761 tcaaacgatt aacggttttc attatttctt tattgtctac actgaccgct tttactactt
    14821 cctgttgaga agcagtaggt tgcatcatgc caggtgaatt tttggattca atggttagca
    14881 caggtttttt gctgctatta tctgtaaaaa gcaatacaag taaaaaaaga tgaagtgtta
    14941 cagcaaaaag aaaagctttt ctataggtaa gatcacttat catgcttgcc cctcatcacc
    15001 agtctgagag tcagtaagca accccacttg ctcagcccct gcttgcttta agagtgccat
    15061 ggcttgaaca actttcccat aagcaacccc ctgatctccc ttaacaagca cattgagttt
    15121 ttgacctgac tgtttggcta actccagttc tgccgcaact cgtactaata atgcctgagc
    15181 ttcaataggt tctgctggcg tactactaat atttaaaaaa tagctgccta attgattcac
    15241 tgacacaatg ataggttctc tgtcagcagc ctgtaaagtt tgactagctg ctttgggcag
    15301 atctactgta acaccttgtg tcagcattgg agcagtaatc ataaaaataa ccagtaaaac
    15361 caacataaca tcaatgtaag gcactacatt aatttctgag attggacgtt cacgattggt
    15421 tttagatcga ttcattttag gtcttatcct ctaggtgaac cagaagtttg ttgttcaatt
    15481 aaagaaatca gttcttcttg aaataaatca tatttattca gcaaagtatt ggcacgtgtc
    15541 gtatatcggt tgtaggcaat aaccgcagga attgcagtaa atagccctaa agcggtagca
    15601 accagcgctt ctgaaatacc tggtgccacc attgcgatag tggcttgttg ggcatgtccc
    15661 aaagcctgaa atgatgtcat tataccccaa actgtaccaa atagacccac atagggagaa
    15721 atagacccta cagatgcaaa aaatggtaaa tgttgctcca gtttttccgc ttctttggca
    15781 tgacttatct gcattactct ttgtataggc tcaatagata ctttgccttg ttttctgcag
    15841 cgaacaaact ctttaaaacc agcgtgaaag atagcggcca ccccttgtct ttcatcacta
    15901 ttactgtcta catcagcata gagtttgctc aaatctccac aatcccagaa ccttctggta
    15961 aaggtttcag tcatttgctt tctacgatta aagaaccatg ctctttgaaa gattaatgtc
    16021 catgaaatta tcgaagcaat taataatatc aacataacgg actttactac caaacccgct
    16081 tgcataaaat acattagtac atttgcttga ttagccacca ttaatctcca cactgaaagg
    16141 tttcgttatt catttctcat aatattaggt agtcgtttgg gctttaaatt actgccaacg
    16201 caaactactt gaacactggc cctgcaaatt aattgtttat gttgattaga tatctcttga
    16261 ttaaatagaa aactgcacgc tctaaattct ttaatttctg ttttaattgt taattgatca
    16321 tctaacctgg ccggaaatac ataattaatt gtgagttcgt gaatcgcaaa taaaatatcg
    16381 gatttttcga attcacttag aattaaattg ttttgcctta ataattcagt tcgagctctt
    16441 tccaaataac aaaggtagtt agcatgataa acaattccca tataatcaac atcttctgca
    16501 tagactctaa tgttatgctg atgatttgat ataagattca tgtgttattc ctgttcgatc
    16561 gcactaaagc caaaatgctg ataagctttc gaagtggcta ttctaccgcg aggagtgcgc
    16621 attagaaagc cttgttgtat taaaaatggc tccaaaacat cttctatagt ccctttttct
    16681 tcgccaatag cagctgcaat actatctatg cctactggac caccattgaa gtgttctatc
    16741 accgctaaca ataattttct atccattaaa tcaaacccgt gctgatccac ttcaagcatt
    16801 tccaatgctt gttgagccat atcaacagta atgatgccat ttcctttcac ttcagaataa
    16861 tccctgactc ttcttaacag gcgattggct attcgtggtg ttcctctgga gcgtaatgca
    16921 atttctctgg ctccttcggg ttttgtaggg acaccaagca aatgcgcgga gcgtgccaca
    16981 atctgggtaa gcgaatcaac agaataatat tccaaacgtt gcactatacc aaatctatcc
    17041 cttaatggcg aggtaagcaa ccctgctctt gtcgttgctc caattaatgt aaagggaggt
    17101 aactccaatt taatggatcg tgctgctggc ccctctccta tcatgatatc caacttataa
    17161 tcttccatgg ctggataaag aatttcttct ataacaggac ttaatctatg aatttcatca
    17221 ataaatagta catcattttg ttgtagattc gtcaatatag cagcaatatc gccagcacgc
    17281 tcaatcactg gcccagaagt ttgtctgata ttcactccca tttcgtgagc tatgatattt
    17341 gcaagggtag ttttaccaag gccaggtggg ccaaaaataa gaacatgatc caaaggatcg
    17401 tttctctttc ttgccgcatt gataaatatc tgcatctgtg agctaacaga atcttgccct
    17461 acgtactcgc tcaagctaag cggcctgatg gctctatcca ttgcgtcttc cgagacaata
    17521 ctttgactac ttattaggcg atcactttcg agcataagag gttttatcgg gttcattcga
    17581 atgaaagcat tttaacatag gtttatctaa tagcagatct ttgtaagctt atttatttaa
    17641 taattaaatg caattgattt cacgattaag gttgatgatt ttatatgata tcaaaatgat
    17701 attataaagt gtgattctca cttacaatat ccatcagctg ttttcgagct ctaaatatac
    17761 gtgagcgtac agttccgatg gggcaatgca tttcttttgc aatatcctga taagtttgtc
    17821 catcaaatat gtgcagccca taacataatc gtaactcttc agataaacat gacattgctg
    17881 cttctatttg tttctcaaac tctaatccca ttatgtctga ctctggagaa ttatgtggtg
    17941 tagaaaattg ttcatccata tactgcattt ctgaatcagc atataaatta ttggtacgaa
    18001 aata
//
LOCUS       DACTJE010000055        17766 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.055, whole genome shotgun sequence.
ACCESSION   DACTJE010000055 DACTJE010000000
VERSION     DACTJE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17766
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.055"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..977
                     /locus_tag="I9760_001439"
     CDS             <1..977
                     /locus_tag="I9760_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isopenicillin N synthase family oxygenase"
                     /protein_id="HAT5043212.1"
                     /translation="NDKSVITVDLNDPVKSLEYLEEAFQIGTFILKNSPVNFKRLAKV
                     LSDLDTFYNLDFKTKIDISGQISGGGATRGYFPLGVESGLVDVFEKKESYSFGWPDNE
                     IPDSDASNLSLVAPNIYPDHGYINPDDINGLIKDFANAAKKLSRLIEIILFNGKTVLP
                     IDESSDFQSLLRSFRYYKKTEFDPPNTGANSLHTDWNLITLVYADADGYQYRDNEGNF
                     QNIIPEMEDCLIVNFGDFLSAWTGGKIVSPWHQVVEAKKKDVRNSLVFFYYPAASSSA
                     VKNPIYNQGEPLSLFVDQSDPLNRLWDFSKIETMNEMYAGKWKQVSRK"
     gene            complement(1119..1568)
                     /locus_tag="I9760_001440"
     CDS             complement(1119..1568)
                     /locus_tag="I9760_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HAT5043213.1"
                     /translation="MSLLLTSPAFEHNDFIPDEFTCHGLDCPPPLAWENAPPNTKSFV
                     IIMDDPDALGGLWVHWVLFNIPASYNELDAAIDVPDSAISVKNSWGKTGYGGPCPPSG
                     THRYYFRLYALDTFLSLDEKATKKDVEYAMQGHILETSELICKYTKI"
     gene            1800..2651
                     /locus_tag="I9760_001441"
     CDS             1800..2651
                     /locus_tag="I9760_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5043214.1"
                     /translation="MTSYQERQVKIPGFTIALKIWNPKNPNPVLCLHGKMDNAASFDL
                     LAPLLSNRQLVAVDYPGTGLSSHYPEGVVPHWKNDAFLMCHVIKALGWKSFDIIAHSL
                     GSFLATVLAIAQPKQVNKLVFLDILGPTVHIIENSMAYLPLNIESYLISGKQPRTVFS
                     SLDAAIKDRMNIGNISYQAAQALVQRGTKQDDEGWVWTFDPRLRCISATIPHEDEIRA
                     MFRAIDVPVCLILANQGVSYPESVFKGRTQSIKNLTIHRVQGGHHVHMDDPASVAEII
                     SQYLSSK"
     gene            2778..3296
                     /locus_tag="I9760_001442"
     CDS             2778..3296
                     /locus_tag="I9760_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1453 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043215.1"
                     /translation="MPRWFRRIVDEGERLLVEVTDELRDESEVHKAVSQLHLRVEQVL
                     NANRARLEKPVIEAAEGFLHDLGVILEEDSMLRFPHAPVNQYLLAEKCTDIIYNYKPS
                     LESAPGIWNQIKAHINNFIEIIFGLENVLPTTQTMFAKDVSFTRCKRNLSRLKESLES
                     DAPDLLSVLVNG"
     gene            complement(3421..4806)
                     /locus_tag="I9760_001443"
     CDS             complement(3421..4806)
                     /locus_tag="I9760_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5043216.1"
                     /translation="MKKNIIMFIVSCAMFMEAIDTTIINTAIPVMAHSLEVNPIDLKL
                     ALISYLLSLAIFIPISGWIADKYGVKKVFIAAIGVFTLSSLWCGFTQSLWELVLARII
                     QGVGGSLTVPVGRLIILRTCERHELITKMSIVVMIASLGMLLGPLLGGIITDYFSWRW
                     IFWVNIPVGIIAMLLSHKLLPTILPRSVPPLDKSGFIMFGAGLAFLTLGLSLFSETKA
                     DIVYSASITLLSALLLIGYTKHSRSKRNPIIKVTLLNIRTFRIAVFGNLLTRLSFGGI
                     PFLLPLLFQIILEYSPQLSGMLLTPVALGVFLVKPLSFSILRRLGYKKLLLLNTVLVC
                     FSLWSFATINQSTSVIYIGFLTFLYGFFIALQYTGMNSLAYANIDDNDMSYATSIMST
                     VQQLSQSFGVAISALLVSLFTFQVSQHFVLTVKIFHLTFFALGILTILSGLIFTSLKK
                     EDGKELIESPT"
     gene            complement(4835..6091)
                     /locus_tag="I9760_001444"
     CDS             complement(4835..6091)
                     /locus_tag="I9760_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HAT5043217.1"
                     /translation="MLKNITRALSLSLLISHNICANEASSIETISQPTTIQFLEKFGA
                     GFANLEHKNLGDRVQLYLPSHNSKTGIVKLRLPNGSKLSYGEIVMYGGDMFGNPAKPI
                     SNCSEKNRLACFQAQFDALGTLGNKQDKRCSNPLNQITHLERFFTNLAEEIEKSKQNG
                     TNDWDYYKQHDSAISKELNKLTCGGSIISGFIPFGNYIKLAEVNYDHFVPDSLVAYKT
                     GHRFALETALKAYEKKQQGLIDEAHKLLELAYAQNAFANHYLTDSFSAGHMRTPRRAI
                     GHDILLPSVLNLLIANLMHNEDNQHGLNVVNHEGISWLAYGDGYLYKPEAELQRMVIL
                     DAMQRSADSIHSTFMNGVIPEHYPEMDVFPDYSKIEQINDTAPLFKVENGVLLKRVNN
                     KDHYDHHWTKYWSGLVTLIEFQVNKI"
     gene            6212..7546
                     /gene="rlmD"
                     /locus_tag="I9760_001445"
     CDS             6212..7546
                     /gene="rlmD"
                     /locus_tag="I9760_001445"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAT5043218.1"
                     /translation="MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGE
                     MVEFQYTRVKKDFDEGKLLSIIEPSTLRVEPKCPHYQMCGGCSLQHMSAEEQIRFKQS
                     HLLDLLSRYGHTEPQSMLSPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
                     TEINQCPILNSKIDTDIVHLRKLIDTMEDKQCIAQIEVAAGDNEVALIFRNLTPLTEQ
                     DELKIRKFAEQFQYKVFLQPGGLDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQ
                     VNAELNRKMVTQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCSRVIGVEGNKNMVER
                     AYMNAKSNHITNVDFYAANLDDVMEVRNLVNTSFSKVLIDPPRSGALEIVKQIDSIDP
                     ERIVYVSCNPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG"
     gene            7552..9756
                     /gene="relA"
                     /locus_tag="I9760_001446"
     CDS             7552..9756
                     /gene="relA"
                     /locus_tag="I9760_001446"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HAT5043219.1"
                     /translation="MVRVKDTTPLTPDGSIDVEMWLHHLGSKGYLDNLELVRAACTLS
                     QLAGQDHATETGQTCLQQGLSMADLLADLEVDQETLAAAIIFENVHYADLSIDDVEEQ
                     LGHNIAKLVKGIEKMSAMNNFQALNKYPQNKNQIDNIRKMLLAMVDDVRVVLIKLAEC
                     LCILRTAGHLSETVRKQLATEAMEIYAPLANRLGIGAIKWEMEDLAFRHLHPEEYKAI
                     AKGLKAKRLERDSFVNRIVEQLEHQIRATGARHFAVYGRSKHIHSIYKKMKRKNVSLD
                     EIYDATAVRILVDTEAQCYEVLGMVHTLWKQIPAEFDDYIINPKSNGYQSLHTAVEGP
                     EGRVFEVQIRTFHMHDLAEMGVAAHWKYKEGAVSQKESHERKIEWLRDVLAWHQEMAA
                     NKGVAENVTTEFLEDRVYVFTPDGDVLDLPHGVTPLDFAYHVHSNLGHRCRGAKVNGH
                     IVPLTYQLKTGDRVEVLTGKEIKPSRDWINPHLNYLKTARAKAKVLHWFKMQDYEDNV
                     RDGRELLEKELKSLGIKSDKLNEVITALNYKKADDLYASLGRGDIKIGQIVNRLAPPD
                     TSEQNLQKFVRQQQKPEVTGSDLRIEGVGNLLTFMARCCQPVPGDEIVGYITIGRGVS
                     VHRKDCPNIIHASEKQKRRFIQVNWGTATRENYVVDVLIKAFDRSELLKDVTSLLSNE
                     KAHVYALQTHSNKHENMAYITLTVEIDGLNSLSRLLTKLEQIPNVLEARRQL"
     gene            complement(9797..10162)
                     /locus_tag="I9760_001447"
     CDS             complement(9797..10162)
                     /locus_tag="I9760_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xcc1710-like domain-containing protein"
                     /protein_id="HAT5043220.1"
                     /translation="MNINLETAEQHAVQAYSDKKIQINSIIYESSLIVSKKEIITDFV
                     IKDIQDINNSYIDLLLRSKPELIIIGHLQTGKTLPATLISQLSQKRIGIECMSIGAAC
                     RTYNVLLSEHRAVVAGFIF"
     gene            10221..11414
                     /gene="alaC"
                     /locus_tag="I9760_001448"
     CDS             10221..11414
                     /gene="alaC"
                     /locus_tag="I9760_001448"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAT5043221.1"
                     /translation="MSQFPRIKRLPPYVFNTINQLKMEARARGEDIIDFGMGNPDQPT
                     PPHIVNKLIEVAQRPDTHRYSMSKGIPRLRRAMATWYLRHYDVHLDSEKQILATIGSK
                     EGLAHLALAISGPGDTVLVPDPAYPIHTYGFIIAGANVKQIPLIDEVQFLAAVEDAIN
                     RTWPKPKALVLNFPANPSTHCVEYEFFEKVVDLAKRNNIWIIHDLAYADIVFDGYKAP
                     SILQVPGAIDIAIETYSMSKSYNMPGWRVGFACGNEELVAALTRIKSYLDYGTFTPIQ
                     VAAITALEGPDDCVLEIRNLYEKRRNLLCDGLNDIGWEVSKPKGTMFVWAPIPLNYRA
                     MGSLEFSKYLLKEAQVAVSPGIGFGQQGDGYVRFGLIENKDRIRQALRNLKALFKRDG
                     LLKAI"
     gene            11419..12228
                     /locus_tag="I9760_001449"
     CDS             11419..12228
                     /locus_tag="I9760_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043222.1"
                     /translation="MNVIINTESDCDSKEGKLLKSQGLAVLNLLACGGYDLVNPPVGN
                     LLKSSHNLEGDWVILTPMHWQASHNDAVIVALDKDLRVTDEEVKYWFDLYSAYLAEEG
                     MPLYFYDKYTWLLRVDDKPPLNSKPIYQVLNKSLMPELSQLDETMYWQKFFTESQMFF
                     SSNARKSLINGVWAWGSGKLKTKKTISICTDKHFLAVAQAYSSNVTLYDPSVNLSGFE
                     VVLLHGIDSLSELHQVEIKKIPAHWYWNNCVLIKAKSHWFTRLWRSLTHAD"
     gene            12218..13957
                     /gene="recJ"
                     /locus_tag="I9760_001450"
     CDS             12218..13957
                     /gene="recJ"
                     /locus_tag="I9760_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAT5043223.1"
                     /translation="MLIKQRQQPEFTVNLPNVPDVLKRIYITRGISDESQLDKQLQTL
                     LPFHTLKGINEACLRLENALRSEQRILIIGDFDADGATSTALAITALRAMGARFVEYL
                     VPNRFEFGYGLTPAIVEVASKWQPDLIITVDNGIASFEGVDAANKMGIDVLITDHHLP
                     AESLPDACAIVNPNQPGCLFPSKSIAGVGVIFYVMLALRRHLSNCNWFNEINMAEPNM
                     ASFLDLVALGTVADVVGLDQNNRIMVNQGMMRIRQGQCREGIKALIEIAGRDYSRLRE
                     SDLGFAIAPRLNAAGRLDDMSLGIECLITTDPKMARNFCQQLDELNLERRQIESEMKE
                     QAMLALEKLSINDEENKHLPVALCLHDKTWHQGVIGILAGRMKEKYHRPVIAFASVSE
                     HELKGSARSVPDLNIRDVLAAVDKDNPGLILKFGGHAMAAGLSISPELLNDFRSAFVS
                     EVLKHLDLSQCEGEILTDGPLQSHELSLETAQLIQQAGPWGQAFAEPVFDNVFEILDQ
                     RIVGKNHLKMTLISSQGGELLDAIAFNVDLKSWPNHRIKYIHAAYKLDINFYQGRTRL
                     QLLIQAMNPVQQA"
     gene            14038..15030
                     /gene="dusA"
                     /locus_tag="I9760_001451"
     CDS             14038..15030
                     /gene="dusA"
                     /locus_tag="I9760_001451"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAT5043224.1"
                     /translation="MVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGA
                     VQNNPDRALYFNSVEHPLAIQLGGADKSALVKCAIIAEQKGFNEINLNLGCPSDKVQA
                     GRFGACLMKEPEHVADCIRAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLVEAGC
                     DKLIIHARKAWLNGLNPKQNRTIPPVNYDYVYELKKEIPHIFITINGNILTVEEINNH
                     LKHVDGVMLGRLACDNPYQIAEIHQALYPEFIKIKRSQLLMNYLDYWFDQSRANLSLS
                     ILVKPLFNLAFGLPGAKHWKKKLMNIIQEKNTSGFSELIQYLLNLEAINQVEFV"
     gene            15131..>17766
                     /gene="secA"
                     /locus_tag="I9760_001452"
     CDS             15131..>17766
                     /gene="secA"
                     /locus_tag="I9760_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAT5043225.1"
                     /translation="MLSTLIKKMFGSRNERTLRRMEKSVMAINAFEPKMQALSNEELA
                     GKTQEFKERFNNGESLDELLAEAFATVREVSLRTLGLRHFDVQLIGGMVLHEGNIAEM
                     RTGEGKTLVATLPAYLNAISGRGVHIVTVNDYLAKRDSQWMKPIYEFLGLTVGVIYPD
                     MSHKEKQEAYKADIVYGTNNEYGFDYLRDNMAFSLTDKVQRELNFAIVDEVDSILIDE
                     ARTPLIISGAAEDSSELYIKINSLIPQLKKQEEEGDEGDYTIDEKQKQAHLTDAGHLH
                     IEELLTKAKLLDPGESLYHASNIMLMHHVNAALKAHAMFHRDIDYIVKDNQVVIVDEH
                     TGRTMPGRRWSEGLHQAVEAKEGVPIQNENQTLASITFQNFFRMYNKLSGMTGTADTE
                     AYEFQQIYNLEVVVIPTNRSMIRKDEADLVYLTQADKFQAIIEDVRECGVRRQPVLVG
                     TVSIEASEFLSQLLKKENIKHQVLNAKFHEKEAQIIAEAGRPGAVTIATNMAGRGTDI
                     VLGGSLAADLANLPADASEQEKEAVKKEWQKRHDEVIAAGGLRIIGSERHESRRIDNQ
                     LRGRAGRQGDPGSSRFYLSLEDNLMRIFASERVASMMRRLGMQPGEPIEHSLVTRAIE
                     NAQRKLEGHHFDVRKQLLDYDNVANDQRQVIYTQRSSIMAMTDTQEVVEMMREEVMDS
                     LVDTYIPPQSLEDQWDPQALSDVLSDEFKIKAPVPDWIDKDHSIQPEKIKEKILALAI
                     EHYDEKVRKVGRPVISQFEKSIILQTLDNHWREHLAAMDQLRQGIHLRGYAQKDPKQE
                     YKKEAFSLFTMMLDNLKYEVIRILSSVEIQTEEDAHVVEEQRRADQIRKMNLMHESLS
                     ENDEASETQTFRRQEKKIGRNDP"
BASE COUNT         5629 a         3029 c         3750 g         5358 t
ORIGIN      
        1 acaatgataa gtctgttatt actgttgatt taaatgatcc ggtgaagagt ctggaatatc
       61 ttgaagaggc tttccagatt ggtactttta ttctaaaaaa ttcgccggta aatttcaaaa
      121 gattagcgaa agttttatct gatctggaca cattttacaa tttggatttt aaaactaaaa
      181 tcgatatttc cggacaaatt tctggcggtg gtgcaaccag aggctatttt ccactgggtg
      241 tagaaagtgg tcttgttgat gtatttgaaa aaaaggaatc ttactcgttt gggtggcctg
      301 ataatgaaat cccggattca gatgcctcaa atttatcatt agttgctccc aatatctatc
      361 ctgatcatgg ttatatcaac ccagatgata ttaatggatt gataaaagat tttgccaatg
      421 cagcaaaaaa attatccagg ttaattgaaa tcatcctctt caatggaaaa actgtcttac
      481 cgatcgatga atcttcagat tttcaatcgt tgttacgaag cttcaggtat tacaaaaaga
      541 cagaatttga ccctcctaat acaggagcaa attcgcttca cacagattgg aacctgatta
      601 ctttggtata tgcagatgca gatgggtacc aatatagaga taatgagggt aactttcaaa
      661 acataatccc tgaaatggaa gattgcctta tagttaattt tggagatttt ttatcagctt
      721 ggactggtgg aaaaattgtt agtccttggc atcaggtagt tgaggcaaag aaaaaagatg
      781 taagaaattc tttagtcttc ttctattatc cagcagcctc tagctcagct gttaaaaatc
      841 ctatatataa tcaaggtgag cctcttagtt tgttcgttga tcagtctgat cctttaaata
      901 ggttatggga cttttctaaa atagaaacca tgaatgaaat gtatgcggga aagtggaaac
      961 aagtgagtag aaaataggat tcagcttaag tcattaagca atgattatca taacaaaaaa
     1021 taaaaaaata cccttggctg gtatattttt tgttccccaa aaaaataggg ttatgctgta
     1081 tattaggaat cattgttctc ttctaatctc aagagggatt agattttagt atatttacaa
     1141 atgagctcac ttgtctcaag aatatggcct tgcatagcat attcaacgtc tttctttgtt
     1201 gctttttcat ccagagataa aaaagtatcc aaagcatata acctaaaata atatcgatgt
     1261 gttccgcttg gtgggcatgg tccgccgtaa cctgttttgc cccaactatt cttaacgctt
     1321 atcgctgagt cgggtacatc tatcgctgca tccagttcgt tataacttgc cggtatgttg
     1381 aataaaaccc aatgaaccca taatcctcct aacgcatccg gatcatccat aattatgaca
     1441 aaggatttgg tatttggcgg agcgttttcc caagcaagcg gtggaggaca atccaatcca
     1501 tggcaagtaa attcatcagg aataaaatcg ttatgctcaa atgccgggct tgttagtaat
     1561 aaactcaaaa tactctcctt atctctttcg gatacagcat tgtgaatctt aactttttgt
     1621 tatttttccc tactaccctt aaattatatt agcatggtaa ttttggatct gccattcatc
     1681 aaagcgataa agaaattgaa aatattgttc tcttcattga ctcttactcc tcctactcat
     1741 tagtatataa atctcttaaa tatttgtctc aactaaagag ttttaagaag gtatatatct
     1801 tgacatctta ccaagaaaga caagttaaaa tcccgggttt tactattgct cttaaaattt
     1861 ggaatcccaa aaatcctaat ccagtactgt gtttgcatgg caaaatggat aacgcagcaa
     1921 gttttgattt gctggcgcct ttgttatcaa acaggcaact ggttgcagtg gattatcctg
     1981 gaacaggatt gtcaagccat taccctgaag gtgttgtccc tcattggaaa aatgatgctt
     2041 ttctcatgtg tcatgttatt aaggcattag gatggaagag ctttgatatt attgcccact
     2101 cactaggttc tttcctggca actgttctag ccattgctca acctaaacaa gttaataaat
     2161 tggtattttt agatatttta ggaccaacgg ttcatattat tgagaacagt atggcttatt
     2221 tacccctaaa tattgaaagc tatttaatat ccggtaaaca accaaggact gtatttagca
     2281 gtctggatgc tgcaatcaag gaccgtatga atattggtaa cataagctat caggcagctc
     2341 aagccctggt tcaaaggggt acaaaacaag atgacgaggg ctgggtttgg acatttgatc
     2401 cccggttacg ctgcattagc gcaactatac ctcatgaaga tgaaattcga gccatgtttc
     2461 gtgctataga cgtgccggtt tgcctaattc tcgctaacca aggtgtatct taccccgaat
     2521 ctgtttttaa aggacgtacc caatcaataa aaaatttgac cattcatagg gtacagggag
     2581 gacatcatgt gcacatggat gatccagcct ctgtcgcaga aattatttct caatatttaa
     2641 gttccaaata aaactggttt attaaagaat aacaagtata gatggactct tgctctaaat
     2701 gttgatgcag ggatgattga taatgaatga ttttatgatg tataattggg caatctattt
     2761 aatggtggag cattaatatg cctagatggt ttagaagaat agtagatgag ggagaaaggc
     2821 tgcttgtcga agtaactgat gaattgcgtg atgaaagtga agtgcataaa gcagtaagtc
     2881 agttacattt acgtgtcgaa caagttttaa atgccaatcg agctagactt gaaaaaccgg
     2941 ttattgaagc agctgaaggt tttcttcatg atctaggggt catcctggaa gaagattcca
     3001 tgttgagatt tcctcatgca ccagtaaacc aatatctact cgcagagaaa tgcactgata
     3061 ttatttacaa ttacaaaccg tccttagagt cagcaccagg gatttggaat cagatcaagg
     3121 ctcatattaa taattttatt gaaataattt tcggtttaga gaatgtttta ccaacgacgc
     3181 agactatgtt tgcaaaagat gtaagtttca ccagatgcaa aagaaacctt tccagattaa
     3241 aggagtcact tgaaagtgac gcgcctgatt tgttgtctgt gttggtcaat ggttaaatca
     3301 tcattgaatt taattaatgt ccttaagcat taattttttc ttgaatgacc agatactgta
     3361 ttgacatgca ttgtcttggt catcaaggtg tctggcttac aagatacaac tgagatggac
     3421 ctaggtgggt gactcaatca gttcttttcc atcctctttt tttaagctgg taaatatcaa
     3481 tcctgaaaga atcgtaagta tccctaaagc aaaaaaggtt agatgaaaaa tcttgactgt
     3541 taaaacaaaa tgctgcgaaa cctgaaaagt gaataaactg accagtaggg cagaaattgc
     3601 tactccaaaa ctttgggata actgttgaac tgtgctcata atacttgtcg cataactcat
     3661 atcattatcg tcaatattcg catacgcaag ggaattcatc cctgtgtatt gcagagcaat
     3721 gaaaaatcca taaagaaatg ttaaaaaacc aatgtagatg acagaagtgc tttgatttat
     3781 tgtggcaaag gaccagagag aaaaacagac taaaacggta tttaaaagga gtaatttttt
     3841 ataacccaat cttcgtaaga tggagaaaga taatggctta accaggaata cgcctaatgc
     3901 cactggtgta agcaacattc ctgacaactg gggagaatat tctaaaataa tttggaagag
     3961 caaaggtaat aaaaagggaa ttccaccaaa acttaaacga gttaataaat ttccaaacac
     4021 ggcaatacga aatgttctta tatttaacaa agtaactttt ataataggat tccttttgct
     4081 acgagaatgt ttggtatatc caattaacag caaggcggat aagagagtaa ttgaagcgga
     4141 gtaaacgata tccgctttcg tctcactaaa caaagacaag cctaaagtca agaatgcaag
     4201 gcctgctccg aacattataa atcctgattt atccaaaggt ggaaccgatc tgggcaggat
     4261 agttggaagc agtttgtgag ataaaagcat ggcgattatg ccaacaggga tattaaccca
     4321 aaatatccac cgccatgaga aataatcagt aataataccg cctagtaagg gaccaagtag
     4381 catacctagc gaggcaatca tgacgacaat actcattttt gttattaatt cgtgacgctc
     4441 acaagttctc agaataatta aacgtccaac aggcacggtt agggagccgc ccacaccctg
     4501 tataattctg gctaatacta attcccaaag gctttgagtg aatccgcacc ataaggaact
     4561 tagagtaaag acaccaatag ctgctataaa tacttttttt acaccatatt tatcagcaat
     4621 ccatccactt ataggaataa agattgccaa gcttaaaaga tagctaatga gcgcaagttt
     4681 gagatcaatt ggattgacct ctaaactatg agccattacc gggattgcgg tgttgataat
     4741 agttgtatca atagcctcca taaacatggc gcatgataca atgaacataa taatattttt
     4801 cttcataatg cctgttttat ggaagttaga acgtttaaat tttattaacc tgaaactcta
     4861 ttaaagtcac aagcccgctc cagtattttg tccaatgatg atcatagtga tccttattat
     4921 taactcgttt aagcaaaacg ccgttttcaa ctttaaataa tggagctgta tcatttatct
     4981 gttcaatttt tgagtagtcg ggaaacacat ccatctctgg gtagtgttca gggataactc
     5041 cattcataaa agtactatgg atactgtccg cagaacgttg catggcgtct aagatgacca
     5101 ttctttgtaa ttcggcttca ggtttgtata aatagccatc tccataggca agccaactta
     5161 ttccttcatg attcactaca tttaacccat gttgattatc ttcattatgc atcaaattgg
     5221 cgataagaag atttaaaaca gaaggcagta atatatcatg tcctatggct cttcttggag
     5281 tacgcatgtg ccctgcagaa aaagaatcag ttaagtaatg atttgcaaag gcgttttgag
     5341 cataggctaa ttcaaggagc ttgtgagctt catcgataag tccttgctgc tttttctcat
     5401 aagcttttaa tgctgtttct aaagcaaatc tatggcctgt tttataggct acaagtgaat
     5461 cgggaacaaa gtgatcatag ttgacttcag ctaatttaat ataattgcca aagggtataa
     5521 aaccactgat tatacttcca ccacaagtca gtttgtttaa ttccttgcta atagccgagt
     5581 cgtgctgctt ataataatcc caatcatttg ttccgttttg ttttgatttt tcgatctctt
     5641 cagccaaatt agtaaaaaac cgctcaagat gagttatctg attaagaggg ttagagcaac
     5701 gtttgtcttg cttgttacct aaagttccca gagcgtcaaa ctgcgcctgg aaacaagcta
     5761 acctgttttt ctcagagcaa ttggatatgg gttttgccgg atttccaaac atatcaccac
     5821 cgtacatcac tatctcgcca taacttagtt tactcccatt gggtaatctc agttttacaa
     5881 taccagtctt gctgttatgg ctgggcaaat aaagttgaac tctatccccc agatttttat
     5941 gttccagatt tgcaaagcct gcaccaaatt tttctaaaaa ttgaatggtc gttggttgag
     6001 atattgtctc gatagaagat gcttcattgg cgcagatgtt atggcttatt aacaatgaca
     6061 aagacaaagc tcgtgtgata ttcttcaaca ttatgcattc ccttgattat ttggcccttt
     6121 atttgggcat tttttgtttt ttatatccat acaacattct aaaaatgtag catgagaatt
     6181 ttgtaattga taattatttg agaaacattt catgagaaaa gttaagccaa aattgaattt
     6241 aacctcacaa accgctcgga tcgtaaattt aagccatgat ggaaggggga tagcacgagt
     6301 taatggcaaa gcaaccttta ttcagggagc attacccggt gagatggttg agtttcagta
     6361 tacacgggta aaaaaggatt ttgatgaagg taaattatta tcaattatcg agccctctac
     6421 tctcagggtt gaaccaaaat gccctcatta tcaaatgtgt ggaggctgtt ctttgcaaca
     6481 tatgagtgcc gaggaacaaa ttcgtttcaa acaatcccat cttttggatt tgctatccag
     6541 gtatggccat actgaacctc aatcaatgtt gtcgccttta acaagccatc attggaatta
     6601 tagaaataaa gcacgcttaa gtactcgctt tgtagaaaag aaacaaagca ccatggttgg
     6661 gtttagagag agaaataatc caaggttcat tacagaaata aatcaatgtc ctattcttaa
     6721 ttcaaaaata gatactgata ttgttcattt aagaaagtta attgatacga tggaggataa
     6781 gcagtgtatc gcacaaattg aagtggctgc aggtgataat gaagtggcgc tgattttccg
     6841 caatctaacc ccattaacag agcaagatga gttgaaaatc aggaaatttg cggaacaatt
     6901 ccaatataag gtattccttc agccaggtgg acttgattcg gttttttgtt tttatccttc
     6961 agatgcccat gcttatttga gttatgagct gcccgattat caaataactt ttcaatttca
     7021 tcccaacgat tttactcagg taaacgccga gttaaacaga aagatggtaa cccaggcaat
     7081 tcagttaatg gaattaaaaa attctgatat cgtgctagac ttattttgtg gactgggtaa
     7141 tttttcgctt cccatggcta aacattgctc tcgagttatc ggtgtcgaag gcaataagaa
     7201 catggttgaa agagcctata tgaatgccaa gtctaatcat attaccaatg tagattttta
     7261 tgctgctaat cttgatgatg tgatggaagt taggaatctg gttaatacgt cttttagcaa
     7321 ggttttaatc gatcctcccc ggtctggtgc gcttgaaatt gtaaaacaaa ttgattcaat
     7381 tgatcccgag cgtatagtat atgtatcttg taacccgata acattagctc gagatacaga
     7441 tatattggtt aatcaaaaag gttacgtttt gataacagct ggggtaatgg atatgtttcc
     7501 tcatacagcg catgtcgagt ctattgcctt gtttcaaaaa ggataatatg tatggtaaga
     7561 gtaaaagata cgactccgtt gacgccagat ggcagtattg acgtagagat gtggttacat
     7621 catcttggtt caaaaggata tttggataat ctcgaactcg taagagctgc ctgtacttta
     7681 agtcaattgg ctgggcagga tcatgctact gaaacagggc aaacttgttt acaacaaggg
     7741 ttatcaatgg ctgatttact tgctgacctg gaagtagatc aggaaactct ggctgccgct
     7801 attatttttg aaaatgttca ttatgcggat ttatctattg acgatgttga agagcaattg
     7861 gggcacaata tcgctaaatt ggtcaaaggc atagaaaaaa tgagtgccat gaacaatttc
     7921 caggctttaa ataaataccc gcaaaacaaa aatcaaattg acaatattcg caaaatgctt
     7981 cttgcgatgg ttgatgatgt gcgagttgtt ttaatcaagc tggcagagtg tttatgtatt
     8041 ttaagaacag cagggcatct gtctgaaacg gtacgtaaac agctggcaac cgaagccatg
     8101 gaaatttacg cgcctttagc taaccgcttg ggaatagggg caatcaagtg ggaaatggaa
     8161 gatttggctt tccggcattt gcatccagaa gaatataagg cgattgccaa gggattgaag
     8221 gctaaacgat tggaaaggga cagctttgtc aatcgcattg tcgaacagct tgaacatcag
     8281 attcgagcaa caggagctcg ccactttgcc gtttatggtc gctcaaaaca tatccatagt
     8341 atttataaaa agatgaagcg taaaaatgta tcattagacg agatatatga tgcaacagcg
     8401 gttcgtattc tggtagatac tgaagcacaa tgttacgaag tgttaggcat ggttcatact
     8461 ttgtggaagc aaattccagc agagtttgat gattacatca tcaatccaaa atccaatggc
     8521 tatcagtctc ttcataccgc agtagagggt ccggaaggaa gagtttttga agtgcaaatc
     8581 agaacttttc atatgcatga tttagcagaa atgggtgtgg cagcccattg gaaatataaa
     8641 gagggcgcgg tatcacaaaa agagagccat gagcgcaaaa tagaatggtt aagagacgtt
     8701 ttggcgtggc accaggaaat ggcagctaat aaaggtgtcg cagaaaacgt tacgactgag
     8761 tttcttgaag acagagtgta tgtatttact cccgatggcg atgtgttgga tttaccccat
     8821 ggggtgactc ctttggattt tgcttatcat gtgcatagta atttgggaca tcgttgccga
     8881 ggtgccaagg ttaatggtca tattgtccca ttgacttatc aattgaaaac aggagatagg
     8941 gttgaagtac tgactggaaa agaaatcaaa ccatcaaggg attggattaa tcctcatctg
     9001 aattatttaa agaccgcaag agccaaggct aaagtattgc attggtttaa aatgcaagat
     9061 tatgaagata atgtgagaga tggtcgtgag ttattggaga aggaattaaa atctttaggt
     9121 attaaatcgg ataaactcaa tgaagttatt acggcattga attataaaaa ggcggatgat
     9181 ctttatgcca gcttgggacg aggtgatatt aaaataggtc aaattgttaa tcgattggca
     9241 cctccagata cttcagaaca aaatttacag aagtttgtaa gacagcaaca aaaaccagag
     9301 gtcacaggga gtgatcttcg tattgaggga gttgggaact tattgacatt tatggcaaga
     9361 tgctgccaac ctgttcccgg tgatgaaata gtaggataca tcacgattgg aaggggggtt
     9421 tccgttcatc gaaaagattg tcctaatatt atacatgcca gtgaaaagca aaagcgacgg
     9481 tttattcagg tgaattgggg tacagcaaca cgggaaaatt atgttgttga tgtgttgatt
     9541 aaagcctttg atcgctccga gttattgaag gatgttactt cgctgctttc aaatgaaaaa
     9601 gcccatgttt atgctttaca gacccatagc aataaacatg aaaatatggc ttatatcact
     9661 ttaactgttg agatcgatgg tttaaacagt ttgtcgcgct tattaactaa actggagcaa
     9721 attcctaacg tgctggaagc aagaaggcaa ttatagcttt ggtatattta gccagagcct
     9781 tctcaaaaaa ttgatattag aagataaacc ctgcaacaac cgctctgtgt tcgctcaata
     9841 agacgttata agttctacaa gcggcgccta tcgacatgca ttcgatacca attctttttt
     9901 gcgataattg actgattaga gtggcaggta gcgttttgcc tgtttgtaga tgaccaataa
     9961 taattaattc gggtttgctt cttaataata aatcaatata tgaattatta atgtcctgga
    10021 tgtctttaat tacaaaatca gtaatgattt ctttcttaga aacaattaaa ctactttcat
    10081 aaattatgga attaatctga atctttttat cgctgtaagc ttgcactgca tgttgctctg
    10141 cggtttctag attgatattc atgattttaa aaagttcatt agtttataat ggctaacagt
    10201 atatcacaga ggtcaatatt atgagtcaat ttccacgcat taaacgctta cccccttatg
    10261 tatttaatac aataaaccag cttaagatgg aagcgcgggc gcgaggagag gacattatag
    10321 actttggcat ggggaatcct gatcaaccaa ctccacctca tattgttaat aagcttatag
    10381 aggttgctca aaggcctgat acgcatcgat attcaatgtc gaaaggaatt cctagattac
    10441 gtcgagcgat ggcaacttgg tatcttcgac attatgatgt tcatctggac agtgaaaaac
    10501 aaatattagc tacaatagga tctaaagagg gacttgcaca tttggctttg gcaatttcag
    10561 gtccaggtga tacggtgctt gttcctgacc ctgcttatcc tatccatacc tacggattta
    10621 tcattgcggg tgctaatgta aagcaaattc ctttaatcga tgaagttcaa tttttggctg
    10681 cagtagaaga tgctattaac agaacctggc caaaacccaa agccttggtt ttgaattttc
    10741 cggcaaatcc aagtactcac tgtgttgaat atgagttttt tgaaaaggtg gttgatcttg
    10801 ccaagcgtaa taacatttgg attattcatg atttggccta cgcagatata gtttttgatg
    10861 ggtataaagc cccttcgatt ttacaagttc ctggtgccat agacattgca attgaaactt
    10921 actcaatgtc caaatcttat aatatgcctg gttggcgtgt tgggtttgct tgtggtaatg
    10981 aagagttagt agctgcctta acacgtataa aatcctactt ggattacggt acttttactc
    11041 ccattcaggt tgctgcaatt acagcattgg aaggccctga tgattgtgtg ttggaaataa
    11101 gaaatcttta tgaaaaacga cgcaatcttt tatgtgatgg actgaatgac attggttggg
    11161 aagtgagcaa gcctaaaggg accatgtttg tttgggcgcc tattcctctc aattaccgcg
    11221 ctatggggtc tcttgagttc agtaaatatt tattgaaaga agcacaagtt gctgtttcac
    11281 ctggtatagg ttttggacag caaggggatg gttatgtccg attcggctta attgaaaata
    11341 aagacagaat aagacaagcc ttgagaaatt taaaagctct gtttaaaaga gatggtttac
    11401 tgaaggctat ttagtgcaat gaatgtcatt ataaacacag agagcgactg tgatagcaaa
    11461 gagggcaaat tattaaaatc gcaaggtttg gcggtactta atttattagc atgcggcggt
    11521 tatgaccttg taaatccccc tgtaggtaac ttattgaaat cgagccataa tcttgaggga
    11581 gattgggtga ttttaactcc tatgcattgg caagcatcac ataatgatgc tgtcattgtc
    11641 gctcttgata aggatttacg agtaacagat gaagaggtga aatattggtt cgatttgtat
    11701 tctgcctatt tagcggaaga aggaatgccc ctgtattttt atgacaaata cacttggtta
    11761 ttgcgagttg atgataagcc acctcttaat tccaaaccaa tttatcaggt actaaacaag
    11821 tctttgatgc cagaattatc gcaactggat gagactatgt actggcaaaa attttttact
    11881 gaaagtcaaa tgtttttctc ttccaatgct cggaaatcat taattaatgg tgtttgggca
    11941 tggggtagtg ggaaattgaa aactaaaaaa acaatatcaa tttgcacaga caagcatttt
    12001 ctggcagtag ctcaagcata ttcttcaaat gtgactttat atgacccgtc agtgaacttg
    12061 agcgggtttg aggttgttct attacatggc attgattctt taagcgaatt acatcaagtt
    12121 gaaataaaaa aaataccggc acattggtac tggaacaatt gtgttttaat taaagcaaaa
    12181 tctcattggt ttactcgatt atggagaagt ctgacgcatg ctgattaaac aacgacaaca
    12241 gcctgagttt actgtcaatt tacctaatgt acctgatgta ttaaaacgta tttatataac
    12301 acgaggcatt agtgacgaat ctcaacttga taaacaatta caaacattac ttccttttca
    12361 cactttaaaa ggcattaatg aagcttgtct aagattagag aatgctttgc gttcagagca
    12421 acgtattctt attatcggtg attttgatgc cgatggggca acttctacgg cattagccat
    12481 tactgccttg cgtgctatgg gtgcaaggtt tgttgagtac ttggtaccta atcgatttga
    12541 atttggttat ggtcttactc ccgctattgt tgaagtggca agcaaatggc aacctgattt
    12601 aattatcact gttgataatg gtatagccag ttttgaagga gtagatgcgg ctaataaaat
    12661 gggaatagat gtattaatta ctgatcatca tttaccagca gaatcactac ctgatgcttg
    12721 tgcaattgtt aatcccaatc agcctggttg tttatttcca agcaagtcaa ttgcgggagt
    12781 tggcgttatt ttttacgtta tgctggcatt gcgaaggcat ctttccaatt gtaactggtt
    12841 taatgaaatc aacatggctg agcctaatat ggcgagtttt ctggatttgg ttgcattagg
    12901 gactgtagcc gacgttgtgg ggcttgacca aaataacagg atcatggtta atcaaggcat
    12961 gatgcgtatt cgtcaaggac aatgcagaga gggaataaaa gccttgattg aaattgctgg
    13021 tagggattat tccaggctaa gagagtctga ccttggtttt gccatagcgc caagattaaa
    13081 cgccgcaggc cgtttggatg atatgtcgct tggtattgaa tgtttgatta cgacggatcc
    13141 aaaaatggca agaaatttct gtcaacagtt ggatgagttg aatctggaaa gaagacaaat
    13201 agaatctgaa atgaaagagc aagccatgct tgctttggag aaattgtcaa ttaacgatga
    13261 agagaataag cacttaccgg ttgccttgtg ccttcatgat aaaacctggc atcaaggagt
    13321 gataggaatc ttggcaggaa ggatgaagga aaaatatcat cgtcctgtca ttgcatttgc
    13381 ttctgtcagt gagcatgagt taaagggctc agcacgttct gtccccgatt taaacattag
    13441 agatgtattg gcagctgttg ataaagacaa tccaggactg attcttaaat tcggaggaca
    13501 tgctatggct gctggcttaa gtattagccc agaattattg aatgattttc gctcagcatt
    13561 cgtgtcagag gttctgaaac atcttgactt atcacagtgc gaaggagaaa tactgacgga
    13621 cggaccattg caatcccatg aactgagttt ggaaacagca caattaatac aacaagctgg
    13681 tccttggggc caagcgtttg cagaacccgt atttgataat gtatttgaaa ttctggatca
    13741 acgaattgtc ggtaaaaatc atttaaaaat gacattaatt tcctcacaag gaggggagct
    13801 acttgatgcc attgctttta atgtggattt aaaatcctgg cctaatcata ggattaagta
    13861 cattcatgcc gcctataaac tggatatcaa tttttatcag gggagaacaa ggctccaatt
    13921 gcttatccag gcaatgaatc ctgttcagca agcataaact cttaaattga tgctattttt
    13981 gtgtatttct caaacaacaa tgtataatgt tatatttgct ttaaattgat attaaacgtg
    14041 gttaatagta ttttttctaa attatctata gcgccgatga ttgattggac taacagtcca
    14101 tttcgtactc tgatgaggtt attggctcct aacgcccttt tgtatactga aatgcaaaca
    14161 acaggggctg tgcaaaataa tcctgacaga gctttgtatt ttaattctgt tgaacatcct
    14221 ctcgctattc aattgggtgg ggcagataaa tccgcattag ttaagtgcgc cattattgct
    14281 gaacaaaaag ggtttaatga aattaatttg aatttgggat gtcctagtga caaagtacag
    14341 gcgggacgat ttggggcctg cttgatgaag gagccagaac acgttgctga ttgtattcgt
    14401 gcaataaaac aggtagtagg aataccagtc acagcgaaaa cgaggatcgg tattgataac
    14461 caggacagtt atgagttttt cagatctttt tctcttcact tggttgaagc aggttgtgat
    14521 aagcttatta ttcatgctcg taaggcttgg ttaaatgggt taaatccaaa gcaaaataga
    14581 acgattcctc ccgtaaatta tgattatgta tatgaactaa aaaaagaaat tccccatatc
    14641 tttattacaa tcaatgggaa tatattaact gttgaagaaa taaacaacca tttaaagcat
    14701 gtcgatggag taatgctagg acggcttgct tgcgataacc cctatcaaat tgctgaaatt
    14761 catcaagcct tatacccaga attcatcaaa attaaacgta gccagttgtt aatgaattat
    14821 ctagattatt ggtttgatca atctcgagcc aatctctcgt taagcatttt ggttaagcca
    14881 ctttttaatt tagcttttgg attaccggga gccaaacatt ggaaaaagaa attgatgaat
    14941 attattcagg agaaaaatac ttccggtttt agtgagttaa tccagtattt attaaatttg
    15001 gaagcaataa atcaagtcga gtttgtataa gaaataatag agcagatgtc aacatttcgt
    15061 ggaattttat caagatttct agtacattaa cgagtttatt aattggaaca tcctgagggt
    15121 tattgaggcc atgctgagta cgttaattaa gaaaatgttt ggaagccgaa atgaacggac
    15181 attacggcga atggaaaagt ctgtaatggc tattaatgcg tttgagccga aaatgcaggc
    15241 tttatcaaac gaagagctgg ctggaaaaac tcaagaattt aaagagcgtt tcaataatgg
    15301 tgaaagtctc gatgagttac ttgcggaagc ttttgctacg gttagagaag tatcattgcg
    15361 cactctcggt cttaggcatt ttgacgtaca attaataggc gggatggtat tgcatgaggg
    15421 aaatattgct gaaatgcgta ccggggaagg taaaacattg gttgcaacct tgccagccta
    15481 tctcaatgcc attagcgggc gtggtgtaca tatagttact gttaatgatt atcttgcaaa
    15541 aagagatagc caatggatga agcccatata tgaattttta gggcttacag ttggcgttat
    15601 ctatcctgat atgtcgcaca aggaaaaaca ggaagcttac aaagcggata ttgtttacgg
    15661 aactaataac gaatacggtt ttgactattt acgtgacaat atggctttta gtctgactga
    15721 taaggtacaa agagagctta attttgctat tgtcgacgaa gtagattcca ttttgatcga
    15781 tgaagcgaga actccattga ttatatctgg cgcggcagaa gatagttcag agctgtatat
    15841 caaaattaat tctttgatcc cacagttaaa aaaacaagaa gaagagggtg acgaaggcga
    15901 ttatactata gatgaaaagc aaaaacaggc tcatctgact gatgcaggcc atctgcatat
    15961 agaagaactg ctgaccaaag ccaaattatt agatcctggt gagagtcttt atcatgccag
    16021 caatatcatg ttgatgcatc atgtgaatgc ggcactcaag gctcatgcaa tgtttcacag
    16081 agatattgat tatattgtca aagacaatca agtggtgata gtggatgagc ataccggccg
    16141 taccatgcct ggaaggcgtt ggtcagaagg cttgcaccag gcagtcgaag ccaaggaggg
    16201 agtcccaatt caaaatgaaa atcagactct ggcttctata acttttcaga attttttccg
    16261 catgtataat aaattgtcag gaatgacggg tactgctgac acagaagcct atgagttcca
    16321 acaaatatat aatcttgaag tggttgtaat acctaccaat aggtcaatga ttagaaaaga
    16381 cgaggctgat ttggtttatc taacacaggc agataaattc caggcgataa tcgaagatgt
    16441 acgagaatgt ggtgttcgta gacaacctgt cttggttgga acagtttcaa tcgaagcttc
    16501 tgagttttta agtcaactct taaaaaagga gaacataaag catcaggtat tgaatgcaaa
    16561 attccatgaa aaagaagcac aaattattgc tgaagccggg cgccctggtg ctgtaaccat
    16621 tgcgactaac atggctggac gaggaacaga tattgtttta ggtggtagct tggcagctga
    16681 tctggctaat ttgccagctg atgcgagtga gcaagaaaaa gaagcggtta aaaaagaatg
    16741 gcaaaaacgt catgatgaag tgattgcagc aggcggactt cgaattattg gctctgagcg
    16801 tcacgaatca agacgtatcg ataatcaatt aagagggcga gccggtcgcc agggtgatcc
    16861 cggaagtagc cgtttttatt tgtcacttga agacaattta atgagaatat ttgcttctga
    16921 gcgcgtggcc tctatgatgc gtcgtttagg aatgcagcca ggtgagccga ttgaacatag
    16981 tttggttacc agagcaattg aaaatgctca acgcaaactg gaaggacatc attttgacgt
    17041 aagaaagcaa ttgttggatt atgataatgt agcgaatgat cagcggcagg tcatttatac
    17101 tcagcgttcc tctattatgg caatgacgga tacacaggaa gttgttgaaa tgatgagaga
    17161 agaggtaatg gatagtttgg ttgatactta tatcccccct caaagtttgg aggatcagtg
    17221 ggatccacag gctttgtctg atgttttgtc agatgaattt aaaatcaaag cccctgtgcc
    17281 tgactggatt gataaagatc atagtataca acctgagaaa attaaagaaa aaattcttgc
    17341 tttagctata gagcattatg atgagaaagt aagaaaagtt ggaaggccag tcatttcaca
    17401 atttgaaaaa tcaattattt tacaaacttt agataatcat tggcgtgaac acttggctgc
    17461 aatggatcaa ttacgtcagg gaattcattt aaggggttat gctcagaagg atccaaagca
    17521 ggagtacaaa aaagaagctt ttagtttgtt tacaatgatg ctggataatc ttaagtatga
    17581 ggtgatcagg atactatcat ccgttgaaat tcaaacggag gaggatgctc acgttgttga
    17641 agagcaacgt agagcggatc agattagaaa aatgaatctc atgcatgaaa gtttatcaga
    17701 gaatgatgaa gcaagtgaaa cgcaaacttt tagacgacaa gaaaagaaaa ttggccgtaa
    17761 tgatcc
//
LOCUS       DACTJE010000056        17592 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.056, whole genome shotgun sequence.
ACCESSION   DACTJE010000056 DACTJE010000000
VERSION     DACTJE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17592
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.056"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(41..274)
                     /locus_tag="I9760_001453"
     CDS             complement(41..274)
                     /locus_tag="I9760_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAT5043226.1"
                     /translation="MSQRETGHVKWFNEKKGFGFIVNQKGEDIFVHYKDIQGVGFKTL
                     HENDPVTFELDRGPKGLKAQNVVVASESVHTEE"
     gene            523..1155
                     /gene="pyrE"
                     /locus_tag="I9760_001454"
     CDS             523..1155
                     /gene="pyrE"
                     /locus_tag="I9760_001454"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAT5043227.1"
                     /translation="MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGD
                     SLRKLGQFYAKTLLEQEVSFEHLFGPAYKGIPLATATAVALAELGRDITVTFNRKEVK
                     THGEGGQLIGSPLTGRTVIIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGL
                     TEKSTLSEIREQGIEVYSIINLFDLIEFLKNDNQYEQVQKLESYQERYGA"
     gene            complement(1268..1369)
                     /gene="cydX"
                     /locus_tag="I9760_001455"
     CDS             complement(1268..1369)
                     /gene="cydX"
                     /locus_tag="I9760_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAT5043228.1"
                     /translation="MWYFSWILGTGLACVFAVLNAVWLELREEDVKQ"
     gene            complement(1381..2517)
                     /gene="cydB"
                     /locus_tag="I9760_001456"
     CDS             complement(1381..2517)
                     /gene="cydB"
                     /locus_tag="I9760_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAT5043229.1"
                     /translation="MPLDYETLRIIWWLLIGILLIGFAIMDGFDLGVAMWLPWLGRTD
                     IERRILINSIGPTWEGNQVWFILGGGAIFAAWPTLYALSFSGFYLAMLVVLLALILRP
                     VGFKYRSKLDNPIWRAFWDWALFIGGFVPALIFGVAVGNVIQGVPFYFDESLRVFYTG
                     SFFELLNPFALGCGLLSVLMLAMHGAFFIQVKTVGTLQNKAKDRARWSALLVIFLFIG
                     LGAWVYYGIDGYVLKGTVPHDGPSNPSYKDVIIQAGAWFNNYKEYPVSLLVPVLCIIS
                     AFLAIIFANKSVLAFVLSGLSVGTLIATVGVSMFPFILPSSSNPKYSLIVWDSSSSQV
                     TLFIMLVATVLLLPIVLAYTAWVYRVLRGKVTESSIKAEQDSAY"
     gene            complement(2533..4065)
                     /locus_tag="I9760_001457"
     CDS             complement(2533..4065)
                     /locus_tag="I9760_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215302.1"
                     /note="part of the aerobic respiratory chain; catalyzes
                     the ubiquinol to ubiquinone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I ubiquinol oxidase subunit CydA"
                     /protein_id="HAT5043230.1"
                     /translation="MIPAAEVVDLSRLQFALTALYHFLFVPLTLGLSVMLAIMETVYV
                     MTGRDLWKKMVKYWGVLFGINFVLGVATGLTMEFQFGTNWAYYSHYVGDVFGAPLAIE
                     GLMAFFLEATFVGLFFFGWNRLSKLQHLTSTWLLALGTNLSALWILIANGWMQHPVGA
                     YFDYQTMRMEVSSFYEMLFNPVAQAKFVHTISAGYVTGAMFVLSISAYFLLRGRNKAF
                     AKRSMTVAVSFGLASALSVVVLGDESGYVANENQKMKLATMEAMWHTEKAPANLTVFG
                     IPDEKTLTTKYAIDIPYALGIIATRSFNTPLQGIIELIEEGKLKIKDGMIAYDALKVL
                     QKDPKNEQAKAVFTKHSENLGYALLLKKYTENVTDATEEQINQAANDLKPKVLPMFFS
                     FRIMVACGIYFILLFATGFYLSVRHRLEKTSWYHRVAFYSLPLPWIAAELGWIVAEYG
                     RQPWVVEGILPTFMGASSLTSGQVMTSIAGFVLFYTVLAIVEIYLMVKYIRLGPDGMN
                     HH"
     gene            4441..4737
                     /locus_tag="I9760_001458"
     CDS             4441..4737
                     /locus_tag="I9760_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT5043231.1"
                     /translation="MKIHGSFKKSALPNLMQAISLLKNEDEAMQFFTDLCTPAELESM
                     ADRWQIVPLLRQGIPYRTIHEETGVSVTTITRVARCLSFGTGGYRLIAERLEKL"
     gene            4734..5615
                     /locus_tag="I9760_001459"
     CDS             4734..5615
                     /locus_tag="I9760_001459"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAT5043232.1"
                     /translation="MKNRLRLALQKKGRLSDESLNLLQRCGLKFRIKPNALLTHVDNF
                     PIDLLFVRDDDIPTLVFDSLCDGGIVGENVLLEASFNRPDKAYRKILALGSCTCRLSI
                     AVPEAFDYQGAGSLDGRRVATSYPNLLNNYLAERKISAEILTLSGSVEVAPRMGMADV
                     ICDLVSTGQTLEDNKLKEVDTVLNSQAVFIQTQRQLDPDMQELFAMLARRIQAVQQAQ
                     ERKYIVFHAPKAALERIAQKLPGAESPTILPLPGNNEKVAVHVVSSEGVFWNTLETIQ
                     YLGASSILVLPIEKMLE"
     gene            5621..6916
                     /gene="hisD"
                     /locus_tag="I9760_001460"
     CDS             5621..6916
                     /gene="hisD"
                     /locus_tag="I9760_001460"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAT5043233.1"
                     /translation="MLTIKNWQLLSENDKRRCLSRPRDNSAIKENVLEIINQVQLSGD
                     KALYDLTKRFDRVNLQYLQISQEKIRQASIPQNALIAIKQAIETISSYHQSLLPENTE
                     ISTASGITIRNVYRPIQKVGLYVPGGNKTPLVSSLLMQAIPAKVAGCPIKVLCTPPDA
                     EGEINEHILVAARLCGIDTIYAIGGAQAIAAMAYGTESVIKVDKIFGPGNSYVTQAKT
                     LVAIDADGAAIDMPAGPSEVMIIADTEANPEFIAADLLAQAEHGPDSQVILICDECEL
                     ANQVNQQLEIQMRYLSRIEFIKQSLANSRIIICSNQSEQLDIINSYAPEHLIINRKNP
                     EPWVEKIVAAGTVFLGSWAAETMGDYVTGSNHVLPTSGFARNHSGLSTLDFMTRFTVQ
                     AINQEAIRNLGPAAMTLAELEGLDAHANAVQIRLNTLGD"
     gene            6917..8011
                     /gene="hisC"
                     /locus_tag="I9760_001461"
     CDS             6917..8011
                     /gene="hisC"
                     /locus_tag="I9760_001461"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAT5043234.1"
                     /translation="MSILNLVRADLLNSKNYVPGGESARYRSHANELPWSPVTMGEHN
                     LNYYPNIGLQIELQKQLAKRYQIYSDQIILTRGSDDGIDLTTRLFLTAGKDAFMQFPP
                     TFPMYAFYVRLQQAELIECPLDRRTNFRLTLDQIENSWKPNCKIIMFCSPNNPTGNLV
                     DLNLIAKTCELYANQSIIVVDEAYIEFANAPSATSLIGEFENLIVLRTLSKAFGLAGL
                     RLGCIIAQSPIIQAFNKIIAPYSIATPSMELAKRALNNSDWFTKTIEQIKSSRAWVIK
                     KFADNPIIEKIYPTETNFILIQTRFSKQLTTWLAHYGIAVRDFPSSSLLHDHLRITVG
                     HDEQNQLLINTLSSFNADVAGLNYEKDFIY"
     gene            7989..9047
                     /gene="hisB"
                     /locus_tag="I9760_001462"
     CDS             7989..9047
                     /gene="hisB"
                     /locus_tag="I9760_001462"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAT5043235.1"
                     /translation="MKKILFIDRDGTLVEEPFDFQVDSLDKIKLTSGVIPALLQLQKA
                     GFTFIMVSNQNGIGTVAFPEEDFAVCHEFILDLFSSQGILFDEIFICPHTPENNCICR
                     KPKTGLLEPYLKETVFAKQYSWVIGDRDTDKEFADNLGVNFLPISKTHTWEMVASAII
                     NDARKASVQRKTKETTIDLSVQLDSDQTSVIDTPIPFFTHMLEQVAKHGGFDLRLQAS
                     GDLEVDEHHLIEDTAIALGEAIRTALGDKWGINRYGYTLPMDESLATIAIDISGRSFC
                     DFKGQFTREFIGGMATEMIPHFFQSLSSALGATIHIEVTGTNHHHMIEACFKVLGRAL
                     RQACSRTNNYLPSTKGVL"
     gene            9044..9643
                     /gene="hisH"
                     /locus_tag="I9760_001463"
     CDS             9044..9643
                     /gene="hisH"
                     /locus_tag="I9760_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAT5043236.1"
                     /translation="MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAEEIQKASHVILP
                     GVGTARSGMTALQQNGLIDVLRTLTQPLLGICLGMQLLLEYSEEDDIPCLGLIPGVAE
                     LLKAERNHPVPHMGWNNLHWQKTSSLQQGLNNSDYVYFVHSYALKADNYALARCQYHE
                     EFTAVVKKGNFYGMQFHPEKSANVGMVLLNNFLSLESTC"
     gene            9637..10356
                     /gene="hisA"
                     /locus_tag="I9760_001464"
     CDS             9637..10356
                     /gene="hisA"
                     /locus_tag="I9760_001464"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAT5043237.1"
                     /translation="MLVIPAIDLQSGRCVRLKQGRFDQVTQFSVFPIERALHFAKLGA
                     KRLHVVDLDGARSGKMQQLELICSMQKTGIPIQAGGGIRSIEQALECSNAGISQLVIG
                     SLAITNPDLTIQIIEKIKPENIVLALDVRVDTKVPLLAINGWQNNSTSSLWEVVSYYE
                     NYGIKNILCTDIACDGMMNGPNFDLYQQAVEYFPQIAWQASGGVRHMQDITTLNSLGI
                     SAVILGLMLYQDHMNLEELLC"
     gene            10350..11117
                     /gene="hisF"
                     /locus_tag="I9760_001465"
     CDS             10350..11117
                     /gene="hisF"
                     /locus_tag="I9760_001465"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAT5043238.1"
                     /translation="MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGEILPLAAHYSASGA
                     DELVFYDITASSDDRSVCPDWVNKVAATINIPFTVAGGIRTLHQARLILNAGADKISI
                     NSPALENPSLINELSKEFGKQCMVIGVDSQWIVDDYYVYQYTGNENKSINSKRKTREW
                     IYEIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCHVPLIASGGAGKIDDFVEVF
                     KKSHVSGALAASVFHDKILAINDIKLALQTENIEVRL"
     gene            11114..11737
                     /locus_tag="I9760_001466"
     CDS             11114..11737
                     /locus_tag="I9760_001466"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAT5043239.1"
                     /translation="MSDLDINSLDWQKMNGLLPAIIQNAENGKVLMLGYMNQEALIAT
                     LTTGQLTLYSRSRKRLWRKGESSGNSMALQHISADCDNDSLLIQVIPKGPACHLGYST
                     CYQPHNNYTLGFLDGLIELINERAEEKNPNSYTAQLLDSGVNRCAQKLGEESVETVIA
                     AVSNNKEELINEASDLIYHLLVLLKACELSFYDVLLCLQARDRSVHT"
     gene            complement(11826..12551)
                     /locus_tag="I9760_001467"
     CDS             complement(11826..12551)
                     /locus_tag="I9760_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HAT5043240.1"
                     /translation="MKNALKREDAANKKLGVLIYENVQPMDVIGPWEVFATWKTILDA
                     SIDMFLVAESSNEVCCANQISLKPHCEFNNSPQFDYLLVPGGAGRREQVNNPRLISFL
                     KKQAQNTDYLLSVCTGAFLLHAAGLLTHHEATTYWRAMPELKSFEDVQLVEKRIVKSG
                     KVWTTGGISSGIDLAFEFISTIAGNDVAGKIQLLFEYFPECKLYSQPAMVSELPSYYA
                     TKKDEAVLEMNLPDYIKEYFKKS"
     gene            complement(12691..13839)
                     /locus_tag="I9760_001468"
     CDS             complement(12691..13839)
                     /locus_tag="I9760_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HAT5043241.1"
                     /translation="MSLALKIKSIILSFFLVLVTTVACATQVSLRDKIGQMLILGFQG
                     KVVDEKSPIAQAIEKNNIGGVILFDFNQQSQTFDKNIESPEQVNRLNNQLQTLTQKAN
                     RKYHRDNLPLLISVDYEGGNVNRLHPRYGFPEMPSAKDIGSMSLERADASAELMANTL
                     KSTGFNLNFFPELDVNINPDNPIIGKKDRSFSSIPEIVTQYAQLYTEQFIKHQIHCSY
                     KHFPGHGSSLSDSHLGFVDISDTWSEQELIPFLQLLSQPNHCDMVMIAHIVNRKLDMT
                     GLPASLSYQIINGLLRRDLKFDGVVITDDMQMKAITNYYGLETAVTLSINAGADMLIF
                     GNQLVEKFQDSTEIIDMIEQKVRSGEISEQRINEAYQRIVKMKRSFNY"
     gene            complement(13964..15136)
                     /locus_tag="I9760_001469"
     CDS             complement(13964..15136)
                     /locus_tag="I9760_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAT5043242.1"
                     /translation="MKAKVILVSGKQNTVLRGHPWIFPKAIAQYSGKLITGELVEIIG
                     SEGESLGFGAYNEHSLYRVRVLALSNEQINSFDYKSLITHRIKQASLVRRCLDLPNEQ
                     TNAYRLFNSEADGLSGLTIDCFNDYCVIASSAYWVELNKSIIVHVLQELLPHNHIIWL
                     PQNKPLSQDGWKEIHTEKSQGSTQVLEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKL
                     SRGKRVLDLYTFTGGFALHAAKAGALHVTAVDSSVQAIEQAKNNAVLNHLNTIEFIKA
                     DAREYLKMAGEYDVVILDPPKLVPSQKHLQQAKNYYRFLHREAFKYMKPGSLLMTCNC
                     SSALSSQDFCSLVSAQAVAMGKQARILGVYGPASCHPTLAAFPEGNYLTAILLALV"
     gene            15214..16197
                     /locus_tag="I9760_001470"
     CDS             15214..16197
                     /locus_tag="I9760_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043243.1"
                     /translation="MRQFTKYLTIFLFSSSVSVAYAGPWFTGPLLAPAGHTVPKGHTN
                     LEVYGIDVFTDGQYNASGQRISTPKFKTFAANPILTHGFTDWLDVQLTVPYLFNSTRG
                     VHENHLADVSTALGIQILEQKGSPKRIDFRVLLQETIPTGRYDQLNPALLGTDATGLG
                     SYQTLVGLDFQYLLQVFKTHYLRTRFIASRLFSSNVHIDGLSSYGGSINTHGTVKPGI
                     ENNLDLAFEFTLTQHWVAVMEGYIATGKATRFNGILNFNNIGSPPVNVSDLDAAPVVI
                     GNASFREEALAPAIEYNFNENIGVIGGVWFPVSGINTSHYMTYVLAINAFW"
     gene            16204..>17592
                     /locus_tag="I9760_001471"
     CDS             16204..>17592
                     /locus_tag="I9760_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HAT5043244.1"
                     /translation="MRYSSYEMKKNRFTYGLALGALGVVFGDIGTSPLYALKVTLSGI
                     PINQFNILGVLSLIFWSLIIIVSFKYLMIIFRADNDGEGGILALLALMKHKSTKYQPL
                     FYIVAIFGAGLLLGDGMLTPAISVVSAVEGLGTLSDKLYPYVLPIASVILVLLFSLQA
                     KGTARIGYLFGPLILIWFITIAILGILQIVEHPVVLQAINPYHAIAFLVDEGLRGYFL
                     LGGIFLVVTGGEALFADIGHFGKNPIRFSWFFIALPCLLLNYFGQGANLIVRPEEISN
                     PFFMIAPPWFYLPLIIIATVATVIASQAVISATFSLTKQAVLLGLCPKIPIVQTSMLH
                     SGQIYVPQINFILFIGTMAFCLAFKTSDNLAHAYGIAVNLEMLLVDAMVAYAAVSIWR
                     WSTFNVIFLFGLFLLIDLAFLGANTHKFITGGWVPIVLAFFIAFIMYSWRYGLEYLRD
                     NFYMNKEDISKIL"
BASE COUNT         5516 a         3583 c         3265 g         5228 t
ORIGIN      
        1 aaaaaataaa gcgtagcctc accagataaa atcatctggc ttattcttct gtgtgtactg
       61 attcgctagc cactaccaca ttttgtgctt tcaaaccctt aggtcctcga tcaagttcaa
      121 aggtgactgg atcattttca tgcagcgttt taaaaccaac tccctgaatg tctttgtaat
      181 gcacgaaaat atcttcgcct ttttgattaa caataaaacc aaatcctttt ttttcattaa
      241 accatttaac atggcctgtt tctctttgcg acattcgcat ttcccctttg acttacacag
      301 atacatcaaa attgttattt gaataaataa aagttcactt tagcgcaact taaagtgaaa
      361 aaataaatca ctccctaata aggaatttac gctataataa acccttttcc atgcaaggat
      421 aaatcctgca ctatgattag catatcagtt ttttgttgat tgttaaggat ttttttaaaa
      481 tatgtgattg ttttcaataa aattttgtat taggaaacca gaatgaatca taccaaatca
      541 tcttttataa aattagctct ggagtgtcaa gtacttaaat ttggtgaatt tactttaaaa
      601 tcaggacgaa taagtcccta tttctttaac gctggtttat tttatcacgg tgattcctta
      661 cgtaagttag gtcaatttta tgcaaaaacc cttttagaac aagaagtttc ttttgagcac
      721 ttgtttggac cagcgtacaa aggtatccca ctcgctaccg caactgcagt ggccttggct
      781 gagttaggca gggacattac agtgactttt aatcgtaaag aagtaaaaac tcatggagaa
      841 ggcggacaat taattggttc gccgctaaca ggaaggacag tgatcattga cgatgtgatt
      901 acagcgggca cagcattcag agaatcacaa accctgatta aagagaatgg agggattctt
      961 cgtggtgtca ttatcgctct ggacagatgc gaacgaggat taactgagaa atcaaccctg
     1021 tcagaaatca gggagcaagg aattgaagtc tattcaatta tcaatttgtt tgatctaata
     1081 gaatttctga aaaatgacaa tcaatacgaa caggtacaaa aactggagtc ctatcaagag
     1141 cgttatggcg cataatatta ggattacaga ttttccttaa aaaagagtaa gacaatcatc
     1201 actcattgtg ttttcagttg ctgagctatc catgaagatc agcaacttac actgagattg
     1261 cctccgtcta ttgcttgacg tcttcttctc taagttccaa ccatactgca ttaagcaccg
     1321 cgaacacaca agccagtcct gtgcccaaaa tccaggagaa ataccacata gaaaaacctc
     1381 ctaatacgca gaatcttgct ctgctttaat agaactttca gttaccttac ctcgtaatac
     1441 tcgataaacc caagccgtgt atgcaagcac aataggcaat aataataccg ttgccactaa
     1501 cataataaac aaagtaacct ggctggaaga gctatcccaa acgatcaaac tgtatttagg
     1561 atttgaagaa gagggtaata tgaaaggaaa catactaacc cctaccgttg caatcaaagt
     1621 accaacgctc aatccgctca gcacaaaagc caacacactt ttattcgcaa aaataatcgc
     1681 taaaaaagcg gatattatgc ataaaacagg cactaaaagt gatactggat actctttata
     1741 attattaaac catgctccgg cttgaataat gacgtccttg taagaaggat ttgatggccc
     1801 atcatgggga actgttcctt ttaaaacata accatcaata ccataataaa cccaagcccc
     1861 taatccaata aataaaaaaa taaccaaaag agcactccag cgagccctgt cttttgcttt
     1921 attttgcaaa gtccctacag tttttacttg aataaaaaaa gcaccatgca tggcaagcat
     1981 aagcacagat aataatccac accctaaagc aaacggattg agtaattcaa aaaaacttcc
     2041 cgtatagaat actcttaaac tctcatcaaa ataaaatgga acaccttgta tgacatttcc
     2101 aaccgctacc ccaaagatta aagccggaac aaatccaccg atgaataaag cccaatccca
     2161 aaatgctctc catatggggt tatccagctt tgatctgtat ttaaagccaa cgggtcttaa
     2221 aatcaacgcc aaaagaacaa ccagcatagc caagtaaaaa cctgagaaag ataaggcata
     2281 gagagtaggc caagccgcaa aaatagcgcc tccgcccagt ataaaccaaa cctggttacc
     2341 ctcccatgtt gggccaatgc tgttaattaa tatccgtctc tcaatatctg ttctacctaa
     2401 ccagggaagc cacattgcca caccaaggtc aaatccatcc ataatggcaa agccaataag
     2461 caaaatacct attaacaacc accaaattat tctgagtgtt tcataatcca aaggcatttg
     2521 attctccttt catcaatgat gattcatacc atctgggcca agacgtatgt atttgaccat
     2581 caagtaaatc tcaacaattg caagaacagt ataaaacaat acaaaacctg cgattgaagt
     2641 cattacttgt cctgatgtta aagaggaggc ccccataaaa gtaggcagaa taccctcaac
     2701 aacccagggt tgacggccat attctgctac tatccaccct aattcagcag ctatccaagg
     2761 taaaggcaaa gagtaaaatg caaccctgtg gtaccaagat gttttttcca atcgatgtct
     2821 tacagacaaa taaaaaccgg ttgcgaaaag aagtataaaa tatatcccac aagcaaccat
     2881 aattctaaat gagaagaaca ttggcaggac tttaggtttg agatcgttcg ccgcttgatt
     2941 tatttgctcc tcagtagcat ccgtcacatt ctcagtatac ttcttaagca ataaagcata
     3001 accaagattt tcgctatgtt ttgtaaaaac agccttggct tgttcgttct ttggatcttt
     3061 ctgaagaact ttcaatgcat cgtatgcaat catcccatct ttgattttta attttccttc
     3121 ctcaattaat tcaataatac cttgtaaagg ggtattaaac gaacgcgtgg caataatacc
     3181 aagggcataa ggaatatcta ttgcatattt tgtcgttaga gtcttctcat ctggtattcc
     3241 aaaaacagtc aaattggcgg gagctttctc ggtatgccac atcgcctcca tagtagccaa
     3301 cttcattttt tggttttcat tggcaacata accgctctca tcgcccagta ccacaacaga
     3361 caatgccgaa gccaaaccaa aagaaactgc gaccgtcatt gagcgtttgg caaacgcttt
     3421 atttcgcccc cgcaaaagaa aataggcgct tatggataag acaaacatgg ctcctgttac
     3481 atatcccgca ctgatggtat gcacaaactt ggcctgagca acaggattaa acagcatttc
     3541 gtagaaactg ctaacctcca ttctcatcgt ttggtaatca aaataggcgc ctacaggatg
     3601 ttgcatccaa ccattagcaa ttaaaatcca caatgctgaa agattagtgc ctaaagctaa
     3661 taaccatgtt gaggtcagat gctgcaattt tgacaatcta ttccagccaa agaaaaataa
     3721 acccacaaag gtagcttcca gaaaaaaggc cattagccct tcgatcgcca gtggagcacc
     3781 aaatacatct cccacataat gtgaatagta agcccagtta gtaccaaact ggaattccat
     3841 agtcagaccg gttgcaactc ccaaaacaaa attaatccca aacaaaacac cccagtattt
     3901 gaccattttc ttccagagat cacggccagt cattacataa actgtctcca tgatagctaa
     3961 cataaccgat aaccccagcg taaggggtac aaatagaaaa tgatatagtg cagttaatgc
     4021 aaattgtaac cttgataaat caacaacttc agcagctgga atcataaaac tacctcttgt
     4081 tatttggggt aagtactgtt tgttcttctt ttcctaacaa ccattcttta ttactagaca
     4141 actcaggatg cattcctctg acacagaaat accataataa aacgagcaat atcattttaa
     4201 atgaaagggt aaatacaata tctctggtaa gtggtttcat gacatttcct caggatttat
     4261 catatcgaca gtctctgata aaaatattta cttctgcctt ttgattttca gtcaatccag
     4321 tttcccggaa agagacctgt atactctacc ccatccagaa ttttttttca ctaaattatt
     4381 gacaatgcta aagataatca ttatcatcat taaaatgtta caatacgtta atacattaac
     4441 atgaaaatac acggatcttt taaaaaatct gcgctgccta atttgatgca agctatcagc
     4501 ttgcttaaaa acgaagacga agccatgcag ttttttacag acttatgtac tcctgctgaa
     4561 ctagaatcta tggctgatcg atggcaaata gttcctttgc tacgccaggg catcccatac
     4621 agaacgattc atgaagaaac aggggtcagt gtaacaacca tcacgcgagt tgcgcgttgt
     4681 ttaagctttg gtacaggcgg ttatcgttta atagccgaaa gattggagaa gttgtgaaaa
     4741 atcgtttacg tctggcctta caaaaaaaag gccgattatc agatgaatct ttaaatctcc
     4801 tacaacgctg tggtttaaag tttcgtatta aaccaaacgc attattaact catgttgata
     4861 attttccaat cgatttacta tttgttcgcg atgatgacat ccctactctc gtattcgaca
     4921 gtttatgtga tggtggcatc gtgggagaaa atgtgctgct ggaagcttct tttaacagac
     4981 cagacaaagc ctatagaaaa atattggcat taggcagttg tacttgtcgt ttatcgatag
     5041 cagtaccaga agcattcgac tatcaaggag ccggtagttt ggatgggagg cgagttgcaa
     5101 ccagctatcc aaatttactc aacaattatc ttgctgaacg aaaaattagt gcggaaattc
     5161 tgaccttatc cggctcggtt gaggtagcgc cacgaatggg aatggctgac gtcatatgcg
     5221 atttggtatc tacaggccaa acactcgaag ataataaact caaagaagtt gatactgttc
     5281 tcaacagcca ggccgtcttt attcaaactc agaggcaatt ggatcctgat atgcaagagt
     5341 tgtttgccat gcttgccaga agaatacagg ctgtccagca agctcaggaa agaaaataca
     5401 ttgtttttca cgcgcccaag gccgcccttg agcgaattgc tcaaaaatta ccaggagctg
     5461 aatcaccaac cattctccca ttacccggaa ataatgaaaa ggttgccgtg cacgtagtct
     5521 ccagtgaagg tgtgttttgg aatactttag agactatcca atatttaggt gccagctcca
     5581 tccttgtatt acctattgaa aagatgctgg agtaattcat atgctaacga taaaaaactg
     5641 gcaattactg tctgagaacg ataaaaggcg atgcctttca aggccacgag acaattctgc
     5701 aataaaggaa aatgtgctgg aaattataaa tcaggtccaa ttatcaggag ataaagcact
     5761 ttacgattta accaaacggt ttgatagagt caatttacaa tatttacaga tctcacagga
     5821 aaaaattaga caagccagca taccgcaaaa tgccttaatc gccataaaac aagccattga
     5881 aacaatctcg tcatatcacc agtctttgtt gcctgaaaat accgagattt cgactgcatc
     5941 aggaattaca atccgcaatg tctacagacc aatccaaaaa gttggcttgt atgtgcctgg
     6001 aggaaataaa acacctttag tttcttcgct tctcatgcaa gcaattccgg caaaggtagc
     6061 gggctgccca ataaaagtgc tttgtactcc ccctgatgca gaaggggaaa ttaacgagca
     6121 tatcctggta gctgcaagat tatgtggcat cgacacaatt tacgctatag gtggagctca
     6181 agccatagcg gctatggctt atggcactga aagtgtcatc aaagtggata aaatattcgg
     6241 gccgggtaat agctatgtca ctcaggcaaa aactttagtt gcaattgacg ccgacggagc
     6301 tgcgattgac atgcccgctg ggccatctga agtgatgatt attgcagata ctgaggcaaa
     6361 ccctgaattt attgctgcag atttattagc ccaggctgaa catggcccgg attcacaggt
     6421 tattttaatt tgtgatgaat gtgaattggc aaaccaggtt aatcaacagc tggaaattca
     6481 gatgagatat ttgtcaagaa tagaatttat caaacaatct ctcgcgaata gcaggataat
     6541 tatctgttct aatcaatcgg aacagctgga tattatcaac tcctacgctc cggaacacct
     6601 gatcattaac agaaaaaacc ccgaaccatg ggttgagaaa atcgtggctg ccggaacagt
     6661 atttcttggc tcttgggccg cagaaacaat gggtgattat gtaacaggaa gcaatcacgt
     6721 cttacctacc agtggttttg ccagaaacca tagcgggctt tctactcttg attttatgac
     6781 acgatttacc gtgcaagcca taaatcaaga ggctatccgt aatttaggtc cagcggcaat
     6841 gactttagcg gaattagagg gtttggatgc tcatgccaat gccgttcaaa taagactcaa
     6901 tactctgggg gattaaatgt ccatactcaa tcttgttcgt gcagatctat taaatagtaa
     6961 aaattatgtc cccggtggtg agagtgctcg ctatcgatca catgccaatg aactgccttg
     7021 gtcaccagtt actatggggg agcacaattt aaattattac ccaaacattg gcttacaaat
     7081 agagctgcaa aagcaattgg caaaacgtta tcaaatctat tccgatcaaa tcatactgac
     7141 cagaggaagt gatgatggca ttgatttgac aaccagactt tttctaaccg ccggaaaaga
     7201 cgctttcatg caatttcccc ctacttttcc tatgtacgct ttttatgttc ggctacaaca
     7261 agcagaattg atcgaatgtc ctttggatag aaggactaat tttcgcctca ccctggatca
     7321 aattgaaaat agctggaaac caaactgcaa aattatcatg ttttgtagtc ccaataaccc
     7381 gactggtaat ttggtggatt taaatttaat tgccaagaca tgtgaactgt atgcgaatca
     7441 atctattata gttgtggacg aggcgtatat agagtttgca aatgcaccaa gcgctacgtc
     7501 cttaattgga gagtttgaaa atctgattgt cttaagaacc ctgtcgaaag cctttgggct
     7561 ggctggatta cgtttaggct gcattatagc ccagagtcct attattcaag cttttaataa
     7621 aatcattgcc ccctattcca ttgccactcc tagtatggag ctggctaaaa gagctttgaa
     7681 taatagcgat tggttcacaa aaactattga gcaaatcaaa tcatctcgtg cctgggttat
     7741 aaaaaaattt gccgataatc caatcattga aaaaatttat cctactgaaa ccaatttcat
     7801 tctcattcaa acacgatttt caaaacaatt aaccacttgg cttgcccatt atggcatcgc
     7861 agtaagagat tttccttcgt cctctttatt gcatgatcac cttcgtatta cggtgggtca
     7921 tgatgagcaa aatcaattat tgattaatac cttatcctca tttaacgctg atgtagcagg
     7981 attaaattat gaaaaagatt ttatttattg accgcgatgg caccttagtt gaagaaccct
     8041 ttgattttca agtagattct ctggataaaa ttaaattaac ttctggagta ataccagccc
     8101 tattgcaatt acaaaaagct ggctttacct tcattatggt atccaatcaa aatggcatag
     8161 gaaccgtagc ttttccagaa gaggattttg ctgtttgcca tgaatttatc ctggatctct
     8221 tttcctctca agggattctt tttgatgaga tttttatatg ccctcacacc ccagaaaaca
     8281 attgtatttg tagaaaacct aaaactggct tgctcgaacc ttacttaaaa gaaacagtat
     8341 ttgccaaaca atattcatgg gtaattggcg accgggatac tgataaagag tttgctgata
     8401 atctgggcgt taatttcttg cccatttcga aaacacatac ttgggaaatg gtggcttctg
     8461 caattatcaa cgatgctcgc aaagcgtctg tgcaaagaaa aacaaaagag acgacaatag
     8521 atttgtcagt tcaactggat agcgatcaaa ccagtgttat agacacccct atccctttct
     8581 tcactcacat gttggaacaa gtagcaaagc acgggggctt tgatttacga ttacaagctt
     8641 ccggagattt ggaagtagat gagcaccact tgattgagga tacagccatt gcattggggg
     8701 aagcaattag aacagcgctg ggtgacaaat ggggaattaa tcgttacggt tatacccttc
     8761 ctatggatga atcattagct accattgcaa tcgatattag cggcagaagt ttttgtgact
     8821 tcaaaggtca gtttactcgt gaatttattg gcggtatggc aacagaaatg atccctcact
     8881 ttttccaatc gctatcgagc gcgttggggg caactatcca tatcgaggta acaggcacca
     8941 atcatcacca catgatcgaa gcatgcttca aagttttagg tagagccttg cgacaggcat
     9001 gctcaagaac gaataattat ctaccttcaa ccaagggtgt attatgattg ctgtgattga
     9061 tgtcagtggg aataatttaa cttctctgac aaatgccttg attcgattgg gagggcattt
     9121 tgcactgacc catgatgctg aagaaataca aaaagcaagc catgtcattt tgccaggggt
     9181 tggaacagca cgttctggta tgacagcttt gcagcaaaat gggctaatag acgttttaag
     9241 aaccttaacc caaccacttc ttggcatctg tcttggaatg caattattgc ttgaatacag
     9301 cgaagaagac gacataccct gcctgggttt gattcctgga gttgcagagt tattaaaagc
     9361 tgaaagaaat catcctgttc ctcatatggg atggaacaat cttcattggc aaaaaacatc
     9421 atcattacaa caaggattaa ataatagtga ttatgtttat tttgttcata gctatgctct
     9481 aaaagcagat aattatgcat tagctcgatg tcagtatcat gaagagttca ctgcagttgt
     9541 aaaaaaaggt aatttttatg gtatgcaatt tcatccagaa aaatcagcca atgttggcat
     9601 ggtattacta aataactttt tatctttgga gtctacatgt tagttattcc agctattgat
     9661 ttgcaatctg gacgatgtgt gcgcttaaaa caaggacggt ttgatcaagt aacgcaattt
     9721 agcgtttttc ccatagaaag ggctttgcat tttgccaaac taggcgccaa acgccttcac
     9781 gtagtcgatt tggatggtgc tcgcagcgga aaaatgcaac agttggagct catttgttcc
     9841 atgcaaaaga caggaatacc aattcaagcc ggaggtggaa tcaggagtat agaacaagct
     9901 ttggaatgca gcaacgcagg aatttcacaa ttggtcattg gcagcctggc tattacaaat
     9961 cctgatttga caatacaaat cattgaaaag attaaacctg aaaatattgt tcttgctctg
    10021 gacgttcgtg ttgatacaaa agtccccctt cttgcgatta atggctggca aaataatagt
    10081 acaagcagcc tttgggaagt agtgtcctat tacgaaaact atgggataaa gaacatactt
    10141 tgtactgaca tcgcatgcga tggcatgatg aatggtccta atttcgattt gtatcaacaa
    10201 gcagttgaat attttcctca gattgcctgg caagcctcag ggggagtacg ccatatgcag
    10261 gatattacaa cgctgaattc attaggaata tccgccgtaa tccttgggct aatgctttat
    10321 caagatcata tgaatttaga ggaattatta tgttaacgaa gcgaatcatt ccctgtcttg
    10381 acgttcgtga taatcaggtt gtcaagggag taaaatttcg caaccataga ataattggag
    10441 agatactccc tttggctgct cactactcag catcaggggc tgatgaatta gtattttatg
    10501 atattactgc aagtagcgat gaccgatctg tttgtcctga ttgggtcaat aaagtagcgg
    10561 ctactatcaa tattccattt acagtggctg gaggcatccg aaccttgcat caagcaagat
    10621 taatattaaa tgccggagca gataaaatct caatcaacag ccctgcgttg gaaaaccctt
    10681 cattaattaa tgaattgagc aaagagtttg gaaaacaatg tatggtaata ggagttgata
    10741 gtcagtggat agttgatgat tattatgtct atcagtatac aggcaatgaa aataaaagta
    10801 tcaattcaaa acgcaaaact cgagaatgga tatatgaaat tcaagaccgc ggtgctggtg
    10861 agatagtcct taattgcatg caatctgatg gcgttcgaat gggatatgat attgcacagc
    10921 tatcagaaat gattaacctc tgccacgttc ccctcatcgc atcgggcggt gctggaaaaa
    10981 ttgatgattt tgttgaggta tttaaaaagt ctcatgtcag tggggctttg gcagcaagcg
    11041 tttttcatga caaaatactg gcaatcaatg acataaaact agcattacaa acagaaaata
    11101 ttgaggtaag actatgagtg acttggacat taactctctt gattggcaaa aaatgaatgg
    11161 actattgccg gcaataatcc aaaacgcaga aaatggtaaa gtattgatgt taggttatat
    11221 gaatcaggaa gcattaattg ccacactaac tacaggacaa ctgaccttat acagcagaag
    11281 tcggaaaaga ctgtggcgta agggagagtc ttccggcaat agtatggctc tacaacatat
    11341 tagtgccgat tgcgacaatg acagtctttt gatccaggtt attcctaaag ggcccgcttg
    11401 tcatttaggt tattcaacct gttatcagcc acataataat tatactcttg ggtttcttga
    11461 tggtttgatt gaattaatta atgaacgtgc tgaggaaaaa aatccaaaca gttatactgc
    11521 tcaactcttg gattctggtg tgaaccgatg tgctcaaaaa ttaggagaag agtcggtaga
    11581 aacagtgatc gctgctgtga gtaacaataa agaagaactt atcaacgaag cgtctgattt
    11641 aatttatcat ttattagtgc ttctcaaagc ttgtgagtta agtttttatg atgttttatt
    11701 gtgtttacag gcaagagaca gaagtgtaca tacttaattg cttattccat agaagatctc
    11761 tataaccaga atcctcagta attatcaatc aagactagtt aattttttaa actactatta
    11821 agaaattaac ttttcttgaa atactcttta atgtaatcag gcaaattcat ttccaacact
    11881 gcctcatcct ttttagttgc atagtaagaa ggcaactcac tcaccatagc aggctgggaa
    11941 tacagcttgc actcagggaa gtattcaaaa agcaactgta tttttcccgc aacatcattg
    12001 ccagcaatgg ttgaaataaa ttcaaatgcc agatcaatgc cactggaaat tccaccagta
    12061 gtccaaactt tacctgactt aacgatacgc ttttcaacca attggacatc ctcaaaagac
    12121 tttaattcag gcatagcgcg ccaataagtt gttgcttcat gatgagtcaa taggcctgcc
    12181 gcatgcaata aaaatgctcc agtacaaaca gaaagcaaat agtcagtgtt ttgagcctgc
    12241 tttttcaaaa aagaaatcaa acgtggatta tttacttgct ctcttctacc agccccacct
    12301 ggaaccagta aataatcaaa ttgtggagaa ttgttaaatt cgcaatgagg tttcaggcta
    12361 atttgatttg cgcaacaaac ttcattgctg ctttctgcca ccagaaacat atcaatagag
    12421 gcatcaagaa tagttttcca ggttgcaaac acttcccacg gcccaataac atccataggc
    12481 tggacgtttt cataaatcaa aactcccaat tttttattcg ccgcatcttc ccttttcagc
    12541 gcgtttttca tgcaatgcca ccttaatctg aaaaatccca ttttttatat tagttttttt
    12601 gataattgac ctgcatcgca tatatacctc agcttatcta tagctcgcag attatcaggt
    12661 acttttatca gagtaaatcg agaaaaatct ctaataatta aaagacctct tcatttttac
    12721 aattcgttga taagcttcat taatcctttg ttcagaaatc tctcccgatc gaactttttg
    12781 ctcgatcatg tcaatgattt ctgtcgagtc ttgaaatttt tctaccaatt gattaccaaa
    12841 tattagcata tctgctcctg cattaattga aagagtaaca gcagtttcta atccataata
    12901 attcgttatt gctttcattt gcatatcatc ggtgataaca accccgtcaa attttaaatc
    12961 acgacgcaat aatccgttaa tgatctggta agaaaggctg gcaggtagcc ctgtcatatc
    13021 cagttttcta ttcacaatat gagctatcat caccatatcg caatgattgg gttgagataa
    13081 taattgtaaa aaagggatta attcttgctc gctccaggtg tcacttatat ctacaaaacc
    13141 caaatgtgaa tcagataatg aactgccatg tccaggaaaa tgtttatatg aacaatgtat
    13201 ttgatgtttt ataaattgct ctgtataaag ttgcgcgtat tgagtgacaa tttccggtat
    13261 cgaagagaaa ctcctgtctt ttttgcctat gataggatta tctggattta tattcacatc
    13321 taattcaggg aaaaaattca aattaaatcc tgttgatttt aaagtatttg ccatcaattc
    13381 tgctgatgca tcagctcttt ccagagacat ggagccaata tcttttgctg aaggcatttc
    13441 agggaatcca taacggggat gcaatcgatt aacgtttccc ccctcgtaat caacagaaat
    13501 aagtaatggt aaattatctc tatgatattt acgattggct ttttgcgtaa gtgtttgtaa
    13561 ctgattattt aaccggttaa cttgttccgg actttctata tttttatcaa aagtttgtga
    13621 ctgctgatta aaatcaaaaa gaataacacc accaatgttg tttttctcaa ttgcctgggc
    13681 aataggtgat ttctcgtcaa ccactttgcc ctggaatcct aatattaaca tttgcccaat
    13741 tttatctctc aaactcactt gtgtggcaca agctaccgta gtcaccaaaa caagaaaaaa
    13801 agataaaata attgacttga ttttcaatgc taaactcatt agaaaatgct taattttctc
    13861 tattgcgata tgcgccaatt atacatatta agaccaaacc aatcaaatgt tatacaaaaa
    13921 aattaaccta ataccatcct tgatttaaaa atcaaggttc cctttatacc aatgccaata
    13981 aaatagctgt taaataattt ccctctggaa aagctgccaa ggtaggatgg cagcttgctg
    14041 gtccgtatac gcctaaaata cgcgcctgct ttcccatcgc aacagcctga gcgcttacca
    14101 gggaacaaaa atcctgggat gacagcgctg aagaacagtt gcaagtcatc aataaagatc
    14161 ctggtttcat atatttaaat gcttctctat gtaaaaatcg gtaataattt ttagcttgct
    14221 gtaaatgttt ttgtgaagga accagttttg gcgggtcaag aatgaccaca tcatattcgc
    14281 cagccatttt cagatattct ctggcatctg ccttaataaa ttcaatggta tttaagtgat
    14341 ttaaaacagc attattttta gcctgctcta ttgcctgcac cgagctatct acagccgtaa
    14401 catgaagagc tcccgcttta gctgcgtgca aagcaaaccc tcccgtaaaa gtataaagat
    14461 ccaggactct ttttcccctg gatagttttg ctatcctttt gtgattttct ctttggtcaa
    14521 gaaacaatcc agttttttgg gtttgcgcaa attcaacatg atacagaaca ccttcttcca
    14581 aaacctgagt gctaccctga cttttctctg tatgaatttc tttccagcca tcttgagata
    14641 atggcttgtt ttgaggaagc caaatgatat gattatgcgg gagtaactct tgtaatacat
    14701 gaacaataat cgatttattc aattctaccc aataggcaga acttgctata acacaatagt
    14761 cattaaagca atcaatcgtt aaaccagaga gtccatccgc ttcactgttg aacaatcgat
    14821 aagcattagt ctgttcattt ggtaaatcaa gacatcgtct caccagactg gcttgcttga
    14881 ttctatgagt aattaaagac ttgtaatcga aactatttat ctgttcattg gagagagcaa
    14941 gaacccttac tctatataaa gagtgctcat tataggctcc gaatcctaaa ctctcacctt
    15001 cagagccaat tatctcaacc aattcacctg taattagctt gccagaatat tgagctatgg
    15061 ctttggggaa aatccaggga tgacctctta atacagtgtt ttgctttcca ctaactaaaa
    15121 taactttcgc tttcattctc tatttcccaa aacctaaaag tcagttaatc tacactatca
    15181 agaattatac ttgcaatgga aagaaggaca aatatgcggc aatttacaaa atacctaacc
    15241 atttttctat tttcatcttc agtgagcgtt gcttatgccg gtccctggtt cactggacct
    15301 ttattggcac ccgcaggcca taccgtcccc aagggccata ccaacctcga agtttatggc
    15361 attgatgtct ttactgatgg gcaatacaat gcatctggac aaagaatatc cacacccaag
    15421 tttaaaacct ttgccgccaa cccaatactc actcatggct ttactgactg gctggatgtg
    15481 cagttgaccg tgccttattt attcaactcg acacgcggag tccatgagaa ccatttggct
    15541 gatgtttcaa ctgcattggg aatacaaatc ctagagcaaa aaggttcccc taaaagaatc
    15601 gattttcgtg tccttttaca ggaaacaata cccactggac gttatgacca attgaatccg
    15661 gctttacttg gtacggatgc cactggtctt ggaagctacc aaacgttagt gggcttagac
    15721 tttcaatatt tattacaagt ttttaaaacc cattatctaa gaacacgatt tattgcatca
    15781 cgcctttttt ccagtaatgt tcatattgat ggactaagta gttatggtgg ttcaataaat
    15841 actcatggga cggttaaacc cggcattgaa aataacttag acttggcctt cgaatttact
    15901 cttactcaac actgggttgc cgtaatggaa ggttatattg caactggtaa agccactcga
    15961 tttaacggta ttttaaactt taacaacatt ggcagcccgc cggtcaatgt cagtgatttg
    16021 gatgctgccc ccgtagttat tggcaacgcc tcatttagag aagaagcact agctccggca
    16081 atagaataca acttcaatga aaacattgga gtaattggtg gagtttggtt tcccgtgagt
    16141 ggaatcaata cttctcatta tatgacctat gtactggcaa ttaatgcctt ttggtgatag
    16201 cacatgcgtt attcctccta tgaaatgaaa aaaaacaggt tcacttatgg ccttgccctt
    16261 ggagctcttg gggttgtatt tggtgatata ggtactagcc cactgtatgc tttaaaagtt
    16321 accttatcag gcattcctat caatcaattc aatatattag gcgtattatc gctcattttt
    16381 tggtcattaa tcatcattgt ttcttttaag tacttgatga ttatttttag ggctgacaat
    16441 gatggggaag gaggaatttt ggcacttctt gctttaatga agcataagag taccaaatat
    16501 caacctctat tttacatagt cgccatattt ggagccggtt tgttattagg tgatggtatg
    16561 cttaccccgg ccatttccgt tgtcagtgcg gtagaaggtc ttggaactct ttcggataag
    16621 ctctaccctt acgttctccc cattgcttct gtgatcctcg ttttgctttt ctctttgcaa
    16681 gccaaaggca cagcaaggat aggctatctt tttgggccac ttatacttat ttggtttata
    16741 accatagcca tattaggaat attgcaaatt gtagagcatc cagtagtttt gcaagcgatc
    16801 aacccctacc atgccattgc atttttggtt gacgaaggcc tgagaggata ttttttactg
    16861 gggggtattt tccttgtagt aacaggaggt gaagccttat ttgcagacat aggtcatttt
    16921 ggtaaaaatc caatccgatt cagctggttt tttatagcgc ttccttgcct gctattaaat
    16981 tattttggcc aaggcgctaa cctgatagta cgccccgaag aaataagcaa cccatttttc
    17041 atgattgcgc caccatggtt ttatctgcct ttaattatta ttgcaactgt tgctacagtg
    17101 atcgcatctc aagcagtaat ttcagcaact ttttcactaa ccaaacaagc cgtgttgtta
    17161 ggtttatgcc ccaaaattcc aattgtccag acctccatgc tacattcagg acaaatctat
    17221 gttccacaaa tcaattttat tctatttatt ggtaccatgg cattttgcct ggctttcaaa
    17281 acctcggata acctggctca tgcttatgga attgctgtaa accttgaaat gcttttagtt
    17341 gatgccatgg ttgcttatgc tgctgtttca atatggagat ggtcaacatt caatgtgata
    17401 tttctctttg gtctattcct tttgattgat ttagcctttt taggagctaa tactcataaa
    17461 ttcattacag gtggctgggt tccaattgtt ttagctttct ttatagcctt cattatgtac
    17521 agttggcgct atgggttaga gtatttaaga gataattttt atatgaacaa agaagatatc
    17581 tctaaaattt ta
//
LOCUS       DACTJE010000057        17096 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.057, whole genome shotgun sequence.
ACCESSION   DACTJE010000057 DACTJE010000000
VERSION     DACTJE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17096
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.057"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..232)
                     /locus_tag="I9760_001472"
     CDS             complement(<1..232)
                     /locus_tag="I9760_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043245.1"
                     /translation="MIGVGLNWHMIDKMRKSKKPHPLNHIASGDNFSHLNLLERGLIS
                     QLIELSYDENTLHTLSLSGLKLLGKHPLLKNTI"
     gene            428..1069
                     /locus_tag="I9760_001473"
     CDS             428..1069
                     /locus_tag="I9760_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAT5043246.1"
                     /translation="MRTSSFLDRLIGEVDSALRTLVLPQKRITTRQSPAENLADTVLS
                     AQEKKHISGLMRVNHAGEVCAQALYQGQALTARLTHIKEQMASAAAEEVDHLAWCEER
                     LYELGSKPSLLNPIWYCGSVLLGALAGLAGDKVSLGFVAETERQVTAHLQRHLHYLPE
                     KDKKTIAILKRMQEDEEHHAHTAMEAGAVELPYIIKQLMNAVSKLMTQSSYYI"
     gene            1086..2081
                     /locus_tag="I9760_001474"
     CDS             1086..2081
                     /locus_tag="I9760_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HAT5043247.1"
                     /translation="MDYNTIFILFVIFIAFVFDFINGFHDAANSIATIVTTGVLTPRQ
                     AVLWAAFFNFIAFLIFNLTVAQTIGYGLIDTDLVDTYFILAALMGAIFWNLVTWYYGL
                     PSSSSHALIGGLAGAAIAKGGLSSLKITGFAKVAAGIFISPVLGLLIGLLFTHIFSLF
                     LRHKNEEELNTLFKSFQLISSALLSITHGGNDAQKTMGIIAVLLFSASWLDGVFYVPF
                     WVVISCHGVISLGTLAGGWRIVHTMGTKITKLNTLKGCAAETGAAITIFAATEFGVPV
                     STTHTVTGSIAGVGLISGIGGAHWKVLRRIFYSWLLTIPAAALVAAAIVNIAPTA"
     gene            complement(2091..3482)
                     /locus_tag="I9760_001475"
     CDS             complement(2091..3482)
                     /locus_tag="I9760_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT5043248.1"
                     /translation="MDLFRKKDIKGSLDNESHLAKCLSVFDLTFLGVGAIIGAGIFVL
                     TGIVAATISGPAVIFSYVVAGFACAFAALSYAELAASIGGCGSAYGYAYAGFGELIAW
                     IVGWDLLLEYSIAVSAVSIGWSGYANDFLMALKIFIPTHLLHGPADGGDLNILAILII
                     AVLTTLLIVGVKSSSRFNNIMVLVKLLVIFIFIAIAFGEVKVENWSSFMPYGWAGVMK
                     GASIIFFAYVGFDAVSTAAEEAINPQRDLPIGIIASLAICTVIYILVSGLLTGIAHYT
                     TLNVSSPISHVLLVLGYKTAAGLVGVGAIAGLTTVMLVLFYGLTRVFLAMSRDGLLPR
                     FLSKTNEHTKTPIRIILLCGILMASLASVTPIGTLAELVNIGTLFAFIVVCGGVLYLR
                     RTHPDMERPFKTPFMPYVPILGIISCFYLILNLPWVTILRFIIWMAVGMAVYFAFSRG
                     NSALSVEGDCAAE"
     gene            3642..4214
                     /locus_tag="I9760_001476"
     CDS             3642..4214
                     /locus_tag="I9760_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAT5043249.1"
                     /translation="MKRLISCLTIICALNASAAAETTSNPCSRWISFLKPVCQRIHQT
                     WTEGHDDMYFSGYAWHNRYVYSNEKIKSYNETAWGGGLGKSLFDEKGNWHGLYAIAFL
                     DSHRHLEPAVGYAYLKTASVNKDLKAGLGYSVLVTSRVDYDNVPIPGALPWAALFYKR
                     ITIAATYIPGSSREGHENGNVLYMLGKISL"
     gene            4354..4779
                     /locus_tag="I9760_001477"
     CDS             4354..4779
                     /locus_tag="I9760_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4949 domain-containing protein"
                     /protein_id="HAT5043250.1"
                     /translation="MMLKTQLTAFIGAVILAGSSLANPIKPEVCPSVPSIQSEGMSMS
                     SEILEGMYITYNLSHYNTSSSWVFIVGPIAAENDDMALAESNKLLSTMSGSPHPEDDG
                     EGNWICQYTTKSKDIIAFAIEADDMLSPLKMMRYLRTIR"
     gene            4926..5468
                     /locus_tag="I9760_001478"
     CDS             4926..5468
                     /locus_tag="I9760_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HAT5043251.1"
                     /translation="MKSFIEQAQFYATYHQNQATRYTHMAGVPLIMLSIMIFLGFVKI
                     VIPGVYATDLACLATLATLIYYFLLNWQLALALTPILIFLLWLASWFNYDGPTKFGLW
                     VFIITFVVGWGLQFYGHFIEGKKPAFMVNFTQALIAPLYLTAELFFMAGLMNSLREQI
                     YGNEEVNTEEISKTKTKSKS"
     gene            complement(5538..7661)
                     /gene="rlmKL"
                     /locus_tag="I9760_001479"
     CDS             complement(5538..7661)
                     /gene="rlmKL"
                     /locus_tag="I9760_001479"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAT5043252.1"
                     /translation="MNYSLFISCSKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPV
                     IYNLCLWSRLANRIQLILFSGHATKEQAVHQLCTDFHWQTVFTHDKTIAIEFHGASEQ
                     IRNTMFGAQIVKDGIVDHFRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSL
                     HQRGYRKKAGKAPLKENVAAAMLLRAKWPELAAQGYSLHDPFCGSGTLVIEAAMMAAH
                     IAPGLLRQDQSLQYWARHQSSLWEKLRTQALQQVKPLAVKLIGTDADSKIITLARSNA
                     ERAGVLPLVEFNTLSLNACRPGTKRGLVVCNPPYGERLGEVTQLVPLYQELGTTLHTC
                     YQGWQAAILTSSPVLAKALGLRADKQYTLYNGPLECKLYCLTLSAANKLKNTPNAPLS
                     DNAQMLFNRLEKNRNHLQKWARKNQITCYRIYDADLPEYAYAIDIYNDYAVLQEYAPP
                     ASIPVHKAEKRSLEMLQVVPRALGIHPEKLIVKQRKQQKGSEQYQKIGKTSQRLIVTE
                     GKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLEPGTRFLNCFCYTASASVHAALAGALT
                     TNVDLSKTYLLWAEDNFRLNDINLSKHQFLQYDCKEWMKTTRDKFDVIFLDPPSFSNS
                     KRMSDILDIQRDHVSLINMAMRLLNPDGVLYFSTNLRQFKLEPMLKEKYAVQDITPQT
                     IDQDFKRNSKIHHCFKIVMPHFADN"
     gene            complement(7658..8137)
                     /locus_tag="I9760_001480"
     CDS             complement(7658..8137)
                     /locus_tag="I9760_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAT5043253.1"
                     /translation="MDEQISKSQKKRDAHYLQDIGIKFIDLSASKLDSLPLPDNLRQA
                     IVDARSIKSHGAKRRQAQLIGKLMRAADFEAILAAYEKILEEESSVTASFHEIELWRD
                     RLLQDGKDALTEFIDAYQPEDVQQLRQLIKKALDDQMKEKNTGAAKALFRYLRSLIK"
     gene            complement(8329..9240)
                     /locus_tag="I9760_001481"
     CDS             complement(8329..9240)
                     /locus_tag="I9760_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAT5043254.1"
                     /translation="MAFFLITLCILILSFFCLAVMVLKFIQQPSLPLSFSQYVKLMCS
                     GVIAFIADTVGVGSFAVNVALAKLLGTFRDDELPAVNNGAQVLPGAIESLFFIYMVDV
                     DLTTLLTLVTGTCIGGLVGGFVVSQLSKQAIRLAMICSFALIIVLLIAHQLRLLPVGG
                     DLTELHSWKLVIGFFAMVVCGSLTSVGVGLFVMVQGVLFLMNVSPVVAFPIMTTAGAM
                     QQPLTTLVFLQKNKIPLKKTLILSFSGCLGVMITIPIFTKLTITWLHFLLLFILIYNF
                     LAIGHTYLRSRPVKQYVQSSVKLAAAD"
     gene            complement(9463..11139)
                     /locus_tag="I9760_001482"
     CDS             complement(9463..11139)
                     /locus_tag="I9760_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M4 family protein"
                     /protein_id="HAT5043255.1"
                     /translation="MLKKTMFFILSISASSIFAADNVDLYQAPLNSINKYPILQTPKN
                     AIILKSSSAVIDNSLQKLNQTKEDNQMIVRYQQLYKGIPVIGAQVMITKGTDSGVQSN
                     DNAEVNGHLLENIELNTKPAISAQQAKEYAKKSYFQFSSQSNIQQETAELQIRPDHNN
                     QLKLVYLVSFKSVQQDGKPDWPFFVIDAQTGALIKQWNNIKNYLDTGPGGNEKVQEYW
                     YGKDGLPALDVTQNGSQCVMENSKVKLVNLHSQWDWENTINTPFEYVCNNNIEENING
                     GFSPGNDAYYFGHVIVGMYKDWYGLNALQHSNGAPMQLVMRVHFGQNYDNAFWDGQAM
                     SFGDGLDFYPLVSLDVAGHEVTHGFTEQHSGLEYHDQSGALNESLSDMAGQASRAYLL
                     EKNPQLYNKAYLQPNEVTWGIGETIVRDSYGKALRFMDYPSSDGSSADCLDKGIAQNN
                     GSYCAISYDEVVAYANTHIALPQERQSFIVHTASGVFNKAFYLMSKDMGIKNAYHIMV
                     VANTKYWTPTTDFKNGACGVIYAARDLNTDINKVKSAFGQVGIDTAGCVI"
     gene            complement(11374..12759)
                     /locus_tag="I9760_001483"
     CDS             complement(11374..12759)
                     /locus_tag="I9760_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAT5043256.1"
                     /translation="MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNID
                     SKNIPYIAWWRDFKDPTLNQLIERGLISNTSLGMSRGSIEAAEGELKKIRYQWVPTLD
                     LMTGYSRNPATGFPGVLIVFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIIS
                     QIAASYFTYLAEIERKEFLQTLANDLTQLAKVASKVYQGGLSSEIEQEELYSQVNLIQ
                     GELEVTERNIVISRNALRYLLNQNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDM
                     QVAENRLRASHEGIGVAESNLLPTIQLDYIGGLAAGDSRYALPKKTVKFNDQLLKAPI
                     IDMPVLGAVAKAKGVNKVSYYNYIDTLQKALRDTTNALSENERLTNKLKQTASAQKHL
                     AKAYNLNDRLYQTGIQSYIETLNSKISLDKINISLNQDKLQELLSIVRLYQELAGGYR
                     ANLESEIKSSV"
     gene            complement(12743..13648)
                     /locus_tag="I9760_001484"
     CDS             complement(12743..13648)
                     /locus_tag="I9760_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAT5043257.1"
                     /translation="MRRWLGVQAFSYVELRSIQISTVFAFTIFVQEWLRYPRAGWTGF
                     AVMMIYAGFDNGTTIFRTYHRFLGVLLGLFTGYLLWFVGHLDYRTLILIMPLMVFFAF
                     FLVGRAYSVPTVFTVNVSVLGSGYFSTEDTLPVTYFIIDYTLCTLIAFTIILGFEYFW
                     FRHYGLMQRFIKSTQAEVIDDLYRLVRLLNQGKIKKTEWFQACIKLTDSLFEVNKLIT
                     NSQFLISSEHAVGDEFNQFVELANRIFIGLKALYMAYYTKHYHTFDYYQLFQQVQKDL
                     VRLKDFVAGEQTIDLSEGVKHATSG"
     gene            complement(13633..14565)
                     /locus_tag="I9760_001485"
     CDS             complement(13633..14565)
                     /locus_tag="I9760_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAT5043258.1"
                     /translation="MFISQLKDKLASYDRYKEHRINGLKAVFVLELMIIFYFFSSIDN
                     PYFYYFYAPITCFTVEAAGTTLKEKYMLLLYTLLGSILSIFLFGIISVYNVLFIFFVF
                     FYTLALYFLVLHHFKKMLVIVPLMLSLASYSLIYGVEADSNFYIAINNALYTLAAMAV
                     IFAGLFIFPKKYYFDIWERAFLEVITGLESLSAKICKGEVKTIAIFSGIIVMERYSKM
                     LPRNIKCYSILKITILSFELILTMSYLLSFQKQLRKEYVIVLHHYLERLCQSCKARQP
                     LILSDQELPAFKETHELEILYQLILSWNYLCADG"
     gene            complement(14540..15613)
                     /locus_tag="I9760_001486"
     CDS             complement(14540..15613)
                     /locus_tag="I9760_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAT5043259.1"
                     /translation="MKEKFATWYKIVTKHIRFFFSYSIPMFFKKAKNYINLPIIVVGL
                     ALIIGLFHLFSYLIPFTDNAFVVTNVTPVAADVSGFITEIYVKNGQKVKKNDPIFSVY
                     RVPYELAYQQAMADYKEGLKKIDVFQKQIERTVALIKATDAELEKAQFALGLKKNKNV
                     AEAVSVLELKNLGYDVSTLSNKRNALQQEVAVLQAKIEQQRHTVASLKAKMDNAKVNL
                     DLTIVRAPGDGVIDNMYVSPNTPIEIHKPVFSFIDTANYYIQANFNETDLRNVRQGDK
                     AYIILRMYYFDKIFHGVVVNSLWAAERQSTAPKSQIQVVTNQNEWLLLPQRFPLQIKI
                     LDPDPDYPLNPGASAYVYISTKR"
     gene            complement(15714..16925)
                     /locus_tag="I9760_001487"
     CDS             complement(15714..16925)
                     /locus_tag="I9760_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAT5043260.1"
                     /translation="MSLEVEPKPHGNNPYANRFDNLPLKDQGRFPRVAYVLQGGGSLG
                     AYQFGVVKGLLEAGYEPDWIAATSIGAIQAAIIVGNPPEKRVEKLERFWDEIAPGNFF
                     DFLGESDITLDFYNKLSAACALLFGQPEFFFPKWCTGTIPLWGDPTTLSYYDTTPLRK
                     TLLDLIDFELLNSCPIRLSLGAVQISNGHLIYFNNINYRIEVEHIMASAALPPGFPAV
                     NIDGELYWDGGVHSNTPLEVILEAIPAENTLCFLIDCFGGRSFIPKNMSEIEERRKDI
                     SYGTHAQRTIYNYLQRQKMRNSMMELSRILTDEQKKESAHLLDIGSPHHCTLVHLAYS
                     SRIVKAASKDYNFGRVVIKKRMEAGYSDAKAVLAEESQWGYLPEDGKSRLYESPNNLS
                     RLFRKRKKEVF"
BASE COUNT         5431 a         3480 c         3073 g         5112 t
ORIGIN      
        1 tgattgtatt ttttaataag ggatgcttgc caagtaattt caatccactt aagcttaaag
       61 tatgtagggt attttcatca taactcaatt caatcaattg gcttattaag cctcgttcta
      121 acaggttaag atggctgaaa ttatctcctg aagcaatatg atttaatgga tgtggctttt
      181 tactcttcct cattttatct atcatgtgcc aatttagacc tacacctatc atttctgtat
      241 aataaagctt aggatcttat tgtgtaataa tcaaaataga tgtataaaat ttaccccatc
      301 aatagctcca tcttaactcg cagccaccga tattaaattg ccttataaaa tttaattgat
      361 taatgacttc aaactacaca ctaatcaagt taacatagag accttacact ttatttttaa
      421 agtatccatg cgaacatcaa gcttcctcga tagattaata ggtgaagtcg atagtgcgct
      481 tcgaaccctc gttttgccac aaaaacgtat tacaacgcgc caatctcccg ctgaaaatct
      541 ggctgatact gtgctttccg ctcaggaaaa aaaacatatt tcaggattaa tgagagtaaa
      601 tcatgccgga gaagtatgtg ctcaagcgct ttatcaagga caagctttaa cagcacgact
      661 tactcacatc aaagagcaaa tggctagtgc cgcggctgaa gaagttgatc atttggcctg
      721 gtgcgaagaa cgtttgtatg aattaggcag caaacccagc cttttaaatc ccatctggta
      781 ttgtggttcg gttctattag gcgccctggc tggattggct ggcgataaag tcagcttggg
      841 atttgttgct gaaacagagc gccaggtaac cgctcattta caaaggcatc tgcattatct
      901 cccggaaaag gataaaaaaa ctatagccat attaaaaaga atgcaagaag atgaggaaca
      961 tcatgcgcat acagcgatgg aagctggagc agttgaactt ccctatatta ttaagcaatt
     1021 gatgaatgct gtatcaaaat taatgactca gagcagttat tatatttaac aggatatgat
     1081 tacatatgga ctacaatacg atattcattc tattcgtcat atttattgcg ttcgtatttg
     1141 attttatcaa cggatttcat gatgccgcca attcgattgc aaccatagtc actacaggtg
     1201 ttttgacgcc aagacaagct gtactatggg ctgctttttt taacttcatt gcttttctga
     1261 ttttcaattt aaccgtagcc caaacaatag gctacggact aatcgatacg gatctggtgg
     1321 atacttattt tattttagca gccctgatgg gagcgatatt ctggaatttg gtgacttggt
     1381 attatggact gccatcaagc tcctctcatg ccttaattgg cggattagct ggtgcggcaa
     1441 tagccaaggg aggcctgtct tcactcaaga ttacaggttt tgccaaagta gcggcaggga
     1501 tatttatatc tcccgttctg ggattattga ttggcttatt atttactcat attttctctt
     1561 tgtttctcag acataagaac gaagaggaac tgaatacatt atttaaaagt tttcaactga
     1621 tatcttcagc attattgagt atcacgcatg gaggaaatga cgcacagaaa acgatgggta
     1681 tcatcgcggt attacttttt tccgcatctt ggcttgatgg tgtattttat gtgccatttt
     1741 gggtcgtcat ttcatgtcat ggggtaatta gtctggggac tttggctggt ggctggagga
     1801 ttgtgcatac catgggaact aaaattacca aacttaacac cctgaagggt tgtgctgctg
     1861 aaacaggagc agccattacc attttcgctg caacagaatt tggagtacct gtctcaacca
     1921 cccatacggt cactggctcc attgcgggag ttgggctaat cagtggcata ggtggcgccc
     1981 actggaaagt tttacgcagg atattctact cctggttgtt gaccattcca gcagcagcct
     2041 tagttgctgc tgctattgta aacatcgcac caacagctta gagtccaatc tcactcggcg
     2101 gcacaatccc cctcaacact caaagcacta tttcccctgc taaatgcaaa atagacagcc
     2161 atacctaccg ccatccaaat tataaaacgc aatatcgtta cccatggcaa atttaaaatt
     2221 aaataaaaac agcttattat gcctagtata ggaacataag gcataaatgg agttttaaat
     2281 ggtctttcca tatcaggatg agttcgcctt aaatataaaa cacctccaca aacaacaata
     2341 aaggcaaaca aggtgccaat attaactagc tccgctagag taccaatagg agttactgag
     2401 gcgagagatg ccattaatat gccacatagc aatataattc gaattggtgt tttagtgtgt
     2461 tcgttcgttt ttgataagaa ccttggcaat aagccatccc ttgacattgc taaaaatact
     2521 ctggttaatc catagaacag aactaacata actgtggtta aaccagcaat tgcgcctaca
     2581 ccgaccagtc cggctgctgt tttataaccc aagacaagca ggacatgact gattggtgat
     2641 gaaacgttaa gggtagtgta atgagcaatc cctgttaata aacctgaaac cagaatatag
     2701 atcactgtac aaatagccag agaagcgatt atccctatgg gtaagtctcg ttgaggatta
     2761 attgcttctt cagcagcagt agaaacagca tcaaacccca cgtaggcaaa aaagatgatt
     2821 gaagcacctt tcataacacc agcccaacca tagggcatga aagaagacca attttcaacc
     2881 ttcacttcac caaatgcgat agctatgaaa ataaaaataa ccaaaagctt aacaagcacc
     2941 atgatattat taaatcgaga actggatttc acaccaacaa ttaataatgt agttaaaaca
     3001 gctatgatca aaatggctaa aatattgaga tccccaccgt cagcgggacc atgtaataaa
     3061 tgagttggaa taaatatttt cagagccatt aaaaaatcat tggcataacc actccagccg
     3121 atggaaacag ccgatacagc aatagaatat tccaataaca aatcccatcc gacgatccac
     3181 gcgattaact caccaaaacc tgcgtaagca tatccataag cactgccaca accccctatg
     3241 ctggcagcca gttcagcata ggataaggcg gcaaaggcgc aggcaaatcc ggcaacaaca
     3301 taggagaata tcacggctgg tccgcttata gtggcagcta ctattcctgt aagaacgaaa
     3361 ataccagcac ctataatcgc gcccactccc aaaaaggtaa ggtcaaaaac actaaggcat
     3421 ttagctaaat gtgattcatt gtctagcgac ccttttatat cttttttacg aaacaagtcc
     3481 atgatagtat gctctcaggt ttatttttta ttgtttattt ccaaaattta agttatcaat
     3541 aaacataaac tccggttatc ttccatctaa caaaacctta cacaattaaa ctctaccatt
     3601 aaattatagc taggagaaac cctgcaaagg ttaaaaattg aatgaaaaga ttaatttcct
     3661 gcttaaccat tatttgcgct ttaaatgcgt ccgctgcagc ggaaacgaca tccaatcctt
     3721 gttctcgctg gataagtttc ctcaagcccg tctgtcaacg catccaccaa acatggacag
     3781 aaggccatga tgatatgtat ttttcaggct atgcctggca taaccgctat gtttacagta
     3841 atgaaaaaat taagtcctac aatgaaaccg catggggtgg tggattaggt aaaagtcttt
     3901 ttgatgaaaa gggaaactgg catgggcttt acgcgattgc cttcctggat tcccatcgcc
     3961 atttagagcc cgccgttggt tacgcctatc tcaaaacagc ttcagttaat aaagatttaa
     4021 aagcaggcct tggatactcg gtattagtta cctctcgggt tgattatgat aatgtcccca
     4081 tccccggagc attaccgtgg gctgctcttt tttataaaag aataacgatc gcagcaacct
     4141 atatacctgg gtccagtcgt gaaggacatg agaacgggaa tgtattgtat atgctcggaa
     4201 aaatatcctt ataaagatgg attaactttt gctagctgca aaaggtcatg tgaaacaaac
     4261 cctgattaga tccctttaaa acggttgcaa ctgaaaattc aacttgttag tcttttgaag
     4321 atttttaact gctaagtaac aaggagaagt aacatgatgt tgaaaaccca gttgactgct
     4381 tttatcggtg ctgtaatctt ggctggctct tctttagcaa atccaataaa acctgaggta
     4441 tgccccagtg taccctctat tcaatcggaa ggaatgtcca tgtcttctga aattttagag
     4501 ggcatgtaca tcacctataa tttaagtcat tacaatacca gttcaagctg ggtgtttatt
     4561 gtagggccaa tcgcagctga aaacgatgat atggcattgg cagaaagcaa taaattactt
     4621 tcaaccatgt cagggtctcc ccatccggaa gatgatggag aaggcaattg gatatgtcag
     4681 tatacaacca aatccaaaga cattattgca tttgccatag aggcagatga tatgctttct
     4741 ccattgaaaa tgatgagata tctcagaaca atccgctgat aagtggataa cacctgcacg
     4801 gacacggaag catgatttgc ttccgtgatt taacgaatag ttaagaactc ctcctctctt
     4861 tttggtactt ctttatttta tgtgctatgt tttcttcatg accctaactt caggatgaaa
     4921 agaaaatgaa atcgtttatt gagcaagctc aattctatgc aacctaccac caaaaccagg
     4981 cgacacgtta tacacatatg gcaggagtcc cgttaatcat gctttctatc atgatttttc
     5041 tgggatttgt taaaatagtt attccaggtg tttatgcaac tgatcttgct tgtttggcta
     5101 cgcttgccac tctcatttat tacttcctat taaactggca acttgccttg gcattaactc
     5161 ccattttgat ctttttgtta tggctggcca gttggtttaa ctatgatggc ccaacgaaat
     5221 tcggactttg ggtatttatt attacttttg ttgtaggctg gggattgcaa ttttacggtc
     5281 attttattga aggaaaaaaa cctgctttta tggttaattt tacccaagcc cttattgcgc
     5341 ctttgtattt aacagcggaa ttgttcttta tggcaggact aatgaattcc ctgagagaac
     5401 aaatttacgg gaacgaagaa gtaaatactg aagaaataag caaaaccaaa acaaaatcca
     5461 aatcttaaag agagtcgagc ttcacgctta tgaaagggaa gctcggcaag agatctaata
     5521 ataaataact gaattattca gttatccgca aaatgcggca ttacaatttt aaaacaatgg
     5581 tgaattttgc tatttcgttt aaaatcctga tcgatggtct gcggagtaat gtcttgtact
     5641 gcatatttct cttttaacat tggctctaac ttaaattggc gcagattagt tgagaaatat
     5701 aaaacaccat caggatttaa caaccgcatt gccatgttaa tcaaagagac atgatctctt
     5761 tgaatgtcta aaatatcgct catacgcttt gagtttgaaa agctgggggg atctaaaaaa
     5821 ataacatcaa atttatccct ggtagttttc atccattcct tacaatcgta ttgcaaaaat
     5881 tgatgcttgg ataaattaat atcatttaat ctaaaattat cttccgccca aagcaaatac
     5941 gttttggata aatcaacatt cgtggttaat gcaccggcca aggcagcatg gacgcttgca
     6001 ctggcggtat aacaaaaaca gttcaaaaat cgagttcccg gctctaattg agcaaatttc
     6061 aagcgcataa gtctatgatc cagaaatagt cctgtgtcaa gataatcata caaattcact
     6121 atcaattttg ccttaccttc tgtcactatc aatctttggc tggttttgcc tattttttga
     6181 tattgttctg agcccttttg ctgctttctt tgtttaacta ttaatttttc agggtgaata
     6241 cctaaggccc gtggaacgac ttgcagcatc tcaaggctgc gtttttctgc cttgtgtact
     6301 ggaatgctgg ctggtggcgc atattcttgc agtacagcat agtcattata tatgtctatg
     6361 gcataggcat attcgggtaa atccgcatcg taaattcgat aacaggtgat ttgatttttt
     6421 cttgcccatt tctgtaaatg attcctgttt ttttccaatc ggttaaatag catttgtgca
     6481 ttatcagaaa gcggggcatt cggggtattt tttagtttat ttgccgcaga aagagtcaaa
     6541 caatagagtt tgcactctaa tgggccatta tataaagtat attgcttatc agctcgtaat
     6601 cctaatgctt tagccaaaac tggactggaa gttaaaatag ccgcttgcca accttgataa
     6661 caagtatgta atgtagtacc caattcttga tataaaggaa caagttgagt tacctccccc
     6721 aatcgctcac cgtaaggtgg attacaaaca actaatcctc tttttgtccc tggtctacaa
     6781 gcatttagcg ataatgtatt aaattcaacc aaaggtaata cgcctgctct ctcagcattg
     6841 gaacgcgcaa gagtaattat tttgctgtca gcgtcagtac caatcagctt aactgccagc
     6901 ggcttcactt gctgtaatgc ctgagttcgc aatttttccc acaaagaact ctgatgccga
     6961 gcccaatatt gtaaggattg atcttgtcgt aataaacctg gagcaatatg agctgccatc
     7021 atggctgctt ctataactaa tgttccagag ccacaaaacg gatcatgtaa gctatatccc
     7081 tgtgcggcta gctctggcca tttggccctt aataacatgg ctgctgcaac gttctccttt
     7141 aaaggagcct tgcctgcttt tttacgatat cctctttgat gcaggctata tccaaccaaa
     7201 tcaaaactca ccgttaaaat atcatttttc aaatgagcgt gaattaaaat ttgcggtttt
     7261 tctttgtcga cagaaggcct tgaaccattc aagcgacgaa aatgatccac tattccatct
     7321 ttgactatct gagcgccaaa catggtatta cgaatttgct cagaggcacc atgaaattca
     7381 atagctatgg ttttatcatg ggtaaaaaca gtttgccaat gaaaatcggt acataactga
     7441 tgtactgctt gctcttttgt ggcatggcca ctaaataaaa taagctgtat tctgttagca
     7501 agtcttgacc ataaacataa attataaatt acaggcaggc tagcctcacc atagacacct
     7561 tgaggactca cctgagtcac atgaagcccc aatcccttga gttcgtcttc cagcaaatac
     7621 tccaaacctt ttgaacaact aatgaataga gagtaattca ttttattaaa gaccttaaat
     7681 agcgaaacag tgctttagca gcacccgtat ttttctcttt catctgatca tctaatgcct
     7741 ttttaatcag ttgccttaat tgttgcacgt cctcaggttg ataagcatcg ataaactctg
     7801 tcaaagcgtc ttttccatct tgtaataacc tgtcgcgcca cagctctatc tcatgaaaag
     7861 aggccgtaac tgaactctct tcttcaagga ttttctcata ggcagcaaga atggcttcaa
     7921 aatcggctgc acgcattaac ttgcctatta attgcgcctg tcgtctttta gccccatggc
     7981 ttttgattga cctggcgtca acaattgctt gacgtaaatt atcgggaagg ggcaaagaat
     8041 ccagtttgga tgcgcttaaa tcaataaatt taatgcctat atcttgtaaa taatgagcat
     8101 ctcttttttt ctgtgattta ctgatttgtt catccatagg gcgatatatt tattaaaata
     8161 acgttaatta tactatataa gtacctatcc agataggata ggctctaatc aattttgaat
     8221 aaaaacaagc aaataccaac atcaacctgc atcatcctat tagttagcca tagctttaaa
     8281 agccctgttc ccgaaaaaac aatgaagaaa aattataaac tgagagactc agtccgctgc
     8341 cgcaagttta acagatgatt ggacgtattg tttgacaggt cttgatcgta aataagtatg
     8401 accaattgct aaaaaattat agataaggat aaataaaagt aaaaaatgca gccaggttat
     8461 tgttaatttt gtaaaaattg gaatggtgat cataacaccc agacatcctg aaaagcttaa
     8521 aatcaaagtc ttttttaacg ggattttatt tttttgtaaa aaaactaatg tggtcagcgg
     8581 ctgttgcatg gctcctgcag ttgtcatgat aggaaaagcg acaactggtg aaacattcat
     8641 taaaaataaa acaccttgca ccatgacaaa aagccctact ccgacagagg taagagagcc
     8701 acaaactacc atagcaaaaa agccaataac cagcttccag gaatgcaatt ctgtcaaatc
     8761 accacctact ggcaatagac gcaattgatg agctattaaa agaacaatga ttagggcaaa
     8821 tgaacaaatc attgccagtc taatggcttg cttgcttaac tggctaacta caaaaccacc
     8881 tactaaccca ccaatacagg ttccggtaac taaagtaagt agcgtagtta aatcaacatc
     8941 taccatgtat atgaaaaata aagattcgat agcgccaggg aggacttgag cgccattatt
     9001 tacagcaggt aattcatcat cacgaaaagt gcctaataat tttgccaagg ctacattaac
     9061 ggcgaaactg ccgaccccca ctgtatctgc aataaaagca ataactccgc tacacattaa
     9121 tttaacatat tgggagaagg atagaggcaa ggaaggctgc tgaataaatt ttaaaaccat
     9181 tacggccagg cagaaaaaac tcaatattag tatgcaaagc gtgattaaaa aaaatgccat
     9241 tctgttccta atacgaaagt aatatataat cccaatgatt ttaaaatcaa agcttattaa
     9301 ggtttctatt ccaaagaaat ctaaaagaaa gatcctgttt gattatatac taatttctat
     9361 gcccagcaaa caacactgcg agcaaaaatg gctcgcgatg aaccacaaga gatcaccgca
     9421 gtttgttgga gcaaaaactt gccgggagct gcttttgctc ccctaaataa cacacccggc
     9481 tgtatcaata cctacttgac caaaagcgga cttaacctta ttgatatcgg tatttaaatc
     9541 cctggcagca taaatgactc cgcaagctcc atttttaaag tctgtcgtag gagtccaata
     9601 ttttgtgtta gcaacaacca tgatgtgata agcgttttta atacccatat ccttagacat
     9661 taggtaaaaa gccttattga acacaccgct ggctgtatga actatgaagc tctggcgttc
     9721 ttgaggaagt gcgatatgtg tattggcata ggctactacc tcatcataac tgatagcaca
     9781 atagctgcca ttgttttgcg caataccttt gtctaaacaa tctgcagagc ttccatcaga
     9841 ggatgggtaa tccatgaatc gcaaagcttt gccataagaa tcacgaacta ttgtttcacc
     9901 aataccccat gtgacttcat tgggctgtaa gtaagctttg ttatacaact gaggattttt
     9961 ttccaaaaga taagctcttg acgcttgtcc tgccatatca gatagggatt cattaagtgc
    10021 acctgattga tcatggtact caagaccaga atgctgctct gtaaaaccat gagtcacttc
    10081 atgaccggct acatctaaag aaaccaatgg gtaaaaatcc aacccatctc caaatgacat
    10141 ggcttgtcca tcccaaaaag cattatcata gttttgccca aaatgaactc gcataaccaa
    10201 ttgcattgga gcaccattag aatgttgtaa ggcattaagt ccataccagt ctttgtacat
    10261 accaacaata acatgtccga aataatacgc atcattgcca ggagaaaatc ctccattaat
    10321 attctcttct atattattgt tacaaacgta ttcaaaagga gtatttatcg tgttttccca
    10381 atcccattga gaatggagat taaccaactt gacttttgag ttttccatga cgcattggct
    10441 gccattttga gtcacatcca aagcaggtaa tccatcttta ccataccaat attcctgaac
    10501 tttctcatta cctccaggcc ctgtatccaa ataatttttg atattgttcc attgcttaat
    10561 caaagctcct gtttgagcat caataacaaa aaaaggccag tctggcttac catcctgctg
    10621 cacgctttta aatgaaacca aataaaccag ctttaattgg ttattatggt ctggccgaat
    10681 ctgtaattca gctgtttcct gttgtatgtt agattgggag ctaaattgaa aataggattt
    10741 ttttgcatat tccttcgctt gttgcgcact aatagccggt ttcgtattaa gttctatatt
    10801 ttccaataaa tgcccgttca cctctgcatt atcattggac tgcactccag aatctgttcc
    10861 tttagtaatc atcacttggg cgccaataac aggtattcct ttatacagtt gttgataacg
    10921 aactatcatt tgattgtctt cttttgtttg atttaatttt tgcaatgaat tatcaataac
    10981 agcagaagaa ctctttaaaa taatcgcatt ctttggtgtt tgtagtatag ggtatttatt
    11041 gatgctattg agaggggctt gatacaaatc tacattgtca gcagcaaaaa tacttgaagc
    11101 agatatcgac aaaataaaaa acattgtttt ttttaacata cacaactctc acaggataca
    11161 caatggcgca aataatgtca caaaattaaa cattatgcaa tatttatttg catatcctgc
    11221 cttgatatat gcccatttat gctcactatg cactaaaaac aaccacaaaa gactgaatca
    11281 gggaggcact gtagaagaag ataagctggg tactatgaaa aatgaaatac gcccctattc
    11341 gtaattaata atttataaca gagttctgtt tgctcagaca gaagacttta tttcagactc
    11401 taaattggcg cgataacctc cagccagttc ttgatataaa cgtacaatgc ttagtagttc
    11461 ttgtaattta tcctgattta aactgatgtt gattttatcg agcgaaattt tgctatttaa
    11521 ggtttctata taactttgaa tacctgtctg atataacctg tcgttcaaat tatacgcttt
    11581 tgccaaatgt ttttgagccg acgcagtttg tttgagctta ttggttaaac gctcattttc
    11641 tgataaggca ttggtcgtat ctcttaatgc tttttgtaaa gtatcaatat aattataata
    11701 agaaacctta tttacgcctt tagccttagc tactgcacct aaaacaggca tatcaataat
    11761 gggggctttc aacaattgat cattaaattt caccgttttt ttcggtaatg cgtagcggct
    11821 atctccagct gccaaaccac caatataatc taattgtata gtaggcaaaa gattactttc
    11881 tgcaacacca attccttcat gtgaagcacg caaacgattt tcagctactt gcatatcagg
    11941 ccgattttca agaactgtta acggtaaaga accgggtatt aatttacgat ttttaagttg
    12001 taataatttt ttaactgttt ttatttcccc tggattttgg tttagtaaat accggagagc
    12061 attgcgactt ataacgatat tacgctctgt tacctcgagt tcgccttgta tcaaattgac
    12121 ctggctatac aactcttcct gctcaatttc agaacttaat cctccttggt aaactttact
    12181 ggcaaccttt gccaactgag ttagatcatt ggctaatgtc tgtaaaaatt ctttgcgttc
    12241 tatttctgca agataggtga aataacttgc cgctatttga gaaataattg ttagtttgag
    12301 agcatcatct tctgctttta cttgtgcaag agtatatttt gctctttttt gctctcttat
    12361 ttgattgatt atgtttattg tatagttagg aataaaaaca atcagaacgc caggaaaacc
    12421 cgttgccgga tttcttgaat aaccagtcat aagatcgagg gtaggcaccc actgataacg
    12481 aattttttta agctcaccct ctgccgcttc aatgctccct cttgacatgc caaggcttgt
    12541 attggaaatc aaccctcttt caatcaactg gttgagagtg gggtctttaa aatcacgcca
    12601 ccaggctatg taaggaatat tcttgctatc gatattttta acattcttgt cttttaccga
    12661 ccatttatta attacttcaa catggggtct gtgataaggc tcactatagc atccgactaa
    12721 aaagactgat aataaaatga ttttaaccgg acgtagcatg ctttactccc tcactaagat
    12781 caattgtttg ctcccctgca acaaaatctt ttaagcgcac caaatctttt tgaacctgtt
    12841 gaaatagttg ataataatca aacgtatgat aatgtttcgt ataataagcc atatataagg
    12901 ctttcaagcc aatgaaaata cgattagcca attccacaaa ctgattaaat tcatccccta
    12961 cagcatgttc tgaactgatt aaaaactggg agtttgttat gagcttattg acctcaaaca
    13021 aactgtcagt caatttgata caagcctgga accattctgt tttctttatt ttaccctgat
    13081 ttagcaaacg aaccagcctg taaagatcat caatcacttc agcctgagta cttttgataa
    13141 atcgctgcat cagtccgtaa tgcctaaacc aaaagtattc aaagcctaga ataatagtaa
    13201 aggcgatcaa cgtgcaaaga gtataatcga taataaaata ggtgactggg agcgtatctt
    13261 ccgtactaaa gtaaccagaa ccaagtactg acacattgac cgtaaataca gtgggaacac
    13321 tgtatgctct tcctaccagg aaaaacgcga aaaaaaccat taaaggcata atcagaatta
    13381 acgttcgata atccagatga cctacaaacc ataatagata tccggtgaat aagcctaaaa
    13441 gcactcctaa aaaccgatga taagtcctga atatggttgt tccattatca aaaccggcat
    13501 aaatcatcat gacagcaaag cccgtccacc cggcccgcgg ataacgcaac cattcctgga
    13561 caaaaatagt aaacgcaaag accgtactga tctgaataga gcgcagctca acataagaaa
    13621 atgcctgtac tcctaaccat cggcgcataa gtaattccag cttaaaatta attgatacaa
    13681 aatttccaac tcatgcgttt ctttaaatgc aggcagctcc tggtctgaga gtatcagtgg
    13741 ttgcctcgct ttacaggatt gacaaagacg ttcaagataa tgatgtaaaa caataacgta
    13801 ttccttacgc agttgctttt gaaaagacag caagtaagac atggtcaaga ttaactcaaa
    13861 agataaaatg gttatcttta atatactgta gcacttaata tttcttggca gcattttgga
    13921 ataacgctcc atcacaataa tgcccgaaaa aatggcgata gttttgactt ctcccttaca
    13981 aatttttgca gatagagatt ccaggccggt aatgacttcg agaaaggcac gctcccaaat
    14041 atcaaaataa tactttttgg gaaaaataaa taatccagcg aagattaccg ccatggctgc
    14101 caaggtatat aaagcattat taatagcaat ataaaaattg ctatctgcct ccacaccata
    14161 aatcagggaa taggacgcca aacttagcat caatggaaca ataacaagca tttttttaaa
    14221 atggtgaagc acgaggaaat aaagagccaa ggtatagaaa aaaacgaaaa aaataaaaag
    14281 cacgttgtat acagaaatta tgccgaataa gaatatagag agtatgcttc ctaaaagggt
    14341 atagagtaaa agcatgtact tctcttttag ggttgttccc gccgcctcca cagtaaaaca
    14401 tgtgattggg gcataaaaat aatagaaata cggattgtca atggaagaaa aaaagtaaaa
    14461 tataatcatc agttccaaaa caaatactgc ttttagtcca ttaatacgat gttctttata
    14521 cctatcgtag gatgcgagct tatcttttag ttgagatata aacataagca ctggcgccag
    14581 gatttaaagg atagtcagga tcgggatcca atatcttgat ttgtaatgga aatcgctgag
    14641 gaagcaacag ccattcattt tgatttgtaa caacctgaat ttgactcttt ggagcagtgc
    14701 tttgtcgttc agcagcccat aacgagttga caacaacccc atgaaatatt ttgtcaaaat
    14761 aatacatgcg taaaatgata tacgctttat caccttgacg tacattgcgt aagtccgttt
    14821 cattaaaatt agcctggata taataatttg cagtatctat aaaagaaaac accggtttat
    14881 ggatctcgat aggggtattt ggagaaacat acatattatc tatcactcca tcaccaggag
    14941 ctctgacaat ggttaaatca agattaacct tggcattatc catttttgcc ttaaggctgg
    15001 cgacagtatg tctttgttgt tcaatcttgg cttgcaaaac cgccacttcc tgttgcagag
    15061 catttctttt attacttaaa gtagaaacat cataaccaag attttttaat tcaagaacag
    15121 atactgcttc ggccacgttc ttattttttt taagaccaag agcaaattga gctttttcaa
    15181 gctcagcgtc cgttgcttta atcaaagcaa ccgttctttc aatctgcttt tgaaacacgt
    15241 ctattttttt taatccttcc ttgtaatcag ccattgcttg ctgataagcc aactcataag
    15301 gaaccctgta aacgctaaat ataggatcgt tcttctttac tttttggcca ttctttacat
    15361 agatttctgt aataaaacca gatacatcag cagctacagg ggtaacatta gtaacaacaa
    15421 acgcattgtc agtgaaaggg atcaaatagg aaaataaatg aaaaaggcca ataataagag
    15481 ccaagccaac gacaataatg ggaagattga tgtaattttt ggccttttta aaaaacatag
    15541 ggatagaata actaaagaaa aaccggatat gtttagttac tattttatac catgtggcaa
    15601 acttctcttt cacaatgcag cgcctctttg atggtgactc tataatacaa catcagttta
    15661 tctctcaaag agagaaaccc catgacaagg ccctcccttt ttcggcatta aaactaaaat
    15721 acctcttttt tccgttttcg aaacaatcga gaaagattgt tgggtgactc atataatcgg
    15781 ctttttccat cctcaggtaa ataaccccat tgagattctt ctgccagcac agccttggca
    15841 tctgaatacc cagcttccat tcgcttctta atgacaactc ggccaaagtt gtaatccttc
    15901 gaagccgctt ttactattct tgaactgtaa gccaaatgaa ccaaggtaca atgatggggg
    15961 cttccaatat ctaacaaatg agcgctctcc ttcttttgtt catccgttaa aatacggctt
    16021 aattccatca ttgagtttcg cattttttgt ctctgcaaat aattgtaaat agtacgttga
    16081 gcatgggttc catatgaaat gtcttttctt ctctcttcaa tttcactcat gtttttggga
    16141 ataaacgatc gcccaccaaa acaatcaatt agaaaacaca aggtattttc tgcaggaatg
    16201 gcctccagaa taacttctaa tggggtgtta gagtgcacac caccatccca atacaattct
    16261 ccatcgatat taacggcagg aaagcccgga ggtaaagcag cacttgccat aatgtgctcc
    16321 acttctatac gataattgat attattaaaa tagattaaat ggccattgct gatttgaaca
    16381 gcaccgagac ttaaacgaat ggggcaggaa tttagcaatt caaaatcaat aagatccagc
    16441 aatgtcttgc gaagaggagt cgtatcataa tagctcagag tcgttggatc accccataaa
    16501 ggaattgttc cggtgcacca ttttggaaaa aaaaactcag gctggccaaa taataaagca
    16561 caagcggcac ttaatttatt ataaaaatct aaagtgatat cagattcacc taaaaaatcg
    16621 aaaaaattac ccggggcaat ttcatcccaa aatctttcga gtttttcaac acgcttttct
    16681 ggaggatttc ctacaataat cgctgcttgt atggcaccaa tagaggttgc tgcaatccag
    16741 tccggttcat acccggcttc aagaagccct ttaaccacac caaattgata ggctcccaaa
    16801 gagccaccgc cttgcagcac ataagccaca cgggggaaac gtccttgatc cttgaggggt
    16861 aagttatcaa agcgattggc ataaggatta ttaccgtgcg gtttaggctc cacttccaaa
    16921 ctcataaaaa ctcctttttc actttagttg atcatcgcat cctggctgaa atgttgggaa
    16981 tacttgctgc cgcttaataa cactgttcaa aatcagattg atatatacat tgttattaaa
    17041 taacgtgagt tatggattac gattatataa gaagctgtcg atatccaggg ttcgtt
//
LOCUS       DACTJE010000058        16873 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.058, whole genome shotgun sequence.
ACCESSION   DACTJE010000058 DACTJE010000000
VERSION     DACTJE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16873
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.058"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(379..1263)
                     /locus_tag="I9760_001488"
     CDS             complement(379..1263)
                     /locus_tag="I9760_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAT5043261.1"
                     /translation="MISEHFICKKAKIIAMSSLFITTVVFAAPSIQYASPMGEENWRM
                     TGNPLRCGLSLTIPNYGIGYFEQYATQSPHFILRKWDQVQRVLPAQIVASPPVWKPLL
                     TRSYLVTRTYIKPGEYGLFLNRQPALKLLTFLSQGYQASFNYLSEEGFRTTVTLSPIR
                     FQKVYSQYQRCLSGLLPFNYEDVKESVFHFGVDSKLLSDADKVQLRRIAQYVDADVQI
                     DAIKIIGHADDSGRKGYNNAISQYRAESVKNYLIRLGVPQRKLSITWVGALQPIARND
                     TDEGRAANRRVVVTLIKK"
     gene            1333..2370
                     /gene="pyrC"
                     /locus_tag="I9760_001489"
     CDS             1333..2370
                     /gene="pyrC"
                     /locus_tag="I9760_001489"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAT5043262.1"
                     /translation="MQTLIINRPDDWHLHLRDGEFLTHTVQASAQHFARALVMPNLKP
                     ALTNLSAIESYRNRIISAIPKQNSFIPYMTFYLNESVKPEELQQAASLSYIVGAKLYP
                     AGATTNSEEGAKSLKALYPLFETLQANNLVLQIHGEVTHSDIFEREALFIDEYLKPIA
                     KHFPKLRIVLEHISTKTAVDYVSEGPANLTATITPHHLLYNRNKLLAGGIRPHYYCLP
                     ILKHEKDQKALQIAATSGNPKFFAGTDSAPHAINAKENACGCAGIYSAPYAVALYTQI
                     FDELNQLKKLNDFLSRFGAEFYQLPINQQQLELIKSPQTIPNSMPFGSTHVVPIAAGD
                     TINWSINGFSQ"
     gene            2354..2977
                     /gene="rnt"
                     /locus_tag="I9760_001490"
     CDS             2354..2977
                     /gene="rnt"
                     /locus_tag="I9760_001490"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAT5043263.1"
                     /translation="MVSRNNILSNTIKNRFRGFLPVVVDVETAGIDPQKNALLEMCIV
                     LLDMDEQGLLKKDSSYFEHILPFPGAELDQKSLEFNQIDPYQPLRFAVDEQTALERLF
                     KPIHDLLKKTRCQRAVLVGHNAWFDLLFIKAAIKRTGIKSPFHAFTCFDTATLAGLIF
                     GETVLAKAAQAAGLHFDSQEAHSAIYDAEKTADLFCLMINKWRMLNI"
     gene            complement(3104..3709)
                     /locus_tag="I9760_001491"
     CDS             complement(3104..3709)
                     /locus_tag="I9760_001491"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin C"
                     /protein_id="HAT5043264.1"
                     /translation="MSVLVGRKAPDFTVAAVMGNGEIVDKFNLHEHLKGKYGLVFFYP
                     LDFTFVCPSELIALDHRIEEFKRRNVEVVAVSIDSHFTHNAYRNTPVKNGGIGPVRFT
                     LAADMTHSICQSYGVEHPVAGVAFRGAFVIDTNGMVRSQIVNDLPIGRNIDEILRIID
                     AVQFFEENGEVCPAGWQKGQAGMKASPKGVAEYLSEHSESL"
     gene            complement(3762..4079)
                     /gene="grxD"
                     /locus_tag="I9760_001492"
     CDS             complement(3762..4079)
                     /gene="grxD"
                     /locus_tag="I9760_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HAT5043265.1"
                     /translation="MDTLEKIKKQIAENAIMLYMKGTPKMPQCGFSARAVQCIEACGV
                     DFAYVDILANPDIRQVLPQFSDWPTFPQLYVKGELIGGSDIIAEMFQQGELEPMLRDA
                     VAA"
     gene            4410..4988
                     /locus_tag="I9760_001493"
     CDS             4410..4988
                     /locus_tag="I9760_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869440.1"
                     /note="SodB; iron binding; present under aerobic and
                     anaerobic conditions; destroys free radicals; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAT5043266.1"
                     /translation="MTFTLPQLPYALDALAPHVSKETLEYHYGKHHNTYVTNLNKLIP
                     GTEFESMTLEEIIMKAKGGIFNNAAQVWNHTFYWHSMSPNGGGEPKGRLAEAINKSFG
                     SFAAFKEQFSQTAATTFGSGWAWLVQDQSGALKIINTSNAGTPMTEGLNALLTCDVWE
                     HAYYIDYRNRRPDYIEAFWSLVNWDFASSNLK"
     gene            5048..6217
                     /locus_tag="I9760_001494"
     CDS             5048..6217
                     /locus_tag="I9760_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HAT5043267.1"
                     /translation="MSLITSYSPMPVTFTHGEGVWLYDEQGKKYLDGLSGIAVCGLGH
                     AHPDVTRTIQEQAARLIHTSNVFHIKEQELLAAKLTSMARMEQVFFANSGAEANEAAI
                     KLTRLFGHKKGIETPSIIVMEKAFHGRTMATLTASGSRKVQAGFEPLVPGFIRAPFND
                     LDAIHTIAANREDVVAVMVEPIQGEGGIYPAEEGYLRALANLCEQNDWMLILDEIQTG
                     NGRTGKLYSCMHYDIQPDILTTAKGLGNGMPIGACLMSKRAKDLFKPGNHGSTFGGNP
                     LACATALTVLEVIERDKLCERVTKNSALLMDKLISNLGEHPHVKAIRGKGYMIGIELD
                     RPANDMRVLGLENQVLFNITAETVVRLLPPLIISEDEIDELVNRITRTINQFTNQ"
     gene            complement(6346..7113)
                     /locus_tag="I9760_001495"
     CDS             complement(6346..7113)
                     /locus_tag="I9760_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT5043268.1"
                     /translation="MTLKITFLGSGSSFAIGPENYQSNVLLELENDTLLIDAGTDIKY
                     SLRDQGLSYKDIKNIYISHLHYDHCGGLEWFALITYFDPNLSHKPCLIASDKIINELW
                     DKSLSGGLSTLPHERANLSAFFDVKAVKQYEGFVWRSIKFKLVQTVHYYSEYELMPSF
                     GLIFSYNKSRILFTSDTQSCPVQLISFYEESDIIFHDCETTESKSGVHAHYSELVKLP
                     SHLKIKMWLYHYNPGKLPNAKKDGFLGFVAKGQSFVF"
     gene            complement(7269..8051)
                     /locus_tag="I9760_001496"
     CDS             complement(7269..8051)
                     /locus_tag="I9760_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAT5043269.1"
                     /translation="MLLKCIEKIIDEYFAVIPRHTPEFENINGALIIAHRGAHNNARG
                     IIENTLEAFQNAKETGCWGIELDVHLTKDKVFVVNHDPTLNRLWNKDIAIADLNFSEL
                     RALECRIPSLSEVVSQYGNSMHLFIELKTAIEEEEILVQTLQHLTPCKDFHLLTLQET
                     TFKKLCSFPRQSLLLVAVHNNVKKFCELSIKEGYGGVLGNYLLLTNRALKYLQDANQH
                     FGVGFVDSKNSLYRELNRGVKWLFTNEAININQYLNDLKNRM"
     gene            8303..9973
                     /locus_tag="I9760_001497"
     CDS             8303..9973
                     /locus_tag="I9760_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAT5043270.1"
                     /translation="MAIKVTKRGMDILRDPILNKGTAFSLQERDEFALHGLIPTTVET
                     LEQQVVRCLDAYSAKEDPLEKHIYLRALQDRNEVLFYRFIIDNLVHILPIIYTPVVGQ
                     ACEMFSHIYRQPRGVFLSYPERDKLDSIIQNIASTRSTKVIVVTDGERILGLGDQGAG
                     GLGIPIGKLSLYTSCGGIHPSNTLPIILDVGTNNKERLDDPEYIGWRHARISGKEYDD
                     FVDQFVQSIKRHMPHVLLQFEDFAQQHAYPLLERYKNQLCTFNDDIQGTASVAVAAIL
                     AATRVTNTPLKEHRVALLGAGSAGCGISEQLVHAMMNQGLSEEEARSRFYLVDRYGLL
                     HDEMTDLLPFQKGFVRSSTSLQNWKLEKKGEITLTDVINNAQPTILLGVSGQPNQFKE
                     AMIKTMLSYCERPIIFPLSNPTSRAEAIPQDLLNWTAGKALIATGSPFEPVVINGHKI
                     EIAQCNNSYIFPGVGLGVVAGQAKRVTDLMMMAAAVALSELAPAIRTGEGRLLPELNS
                     IREVSQHIARAVILQGIKEGHIEPMNNNKIDDSIKRTMWTPQYEPYVL"
     gene            10203..11171
                     /locus_tag="I9760_001498"
     CDS             10203..11171
                     /locus_tag="I9760_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAT5043271.1"
                     /translation="MSIITTNLNRAIHDLQQGKPVAIPTETVYGLAAPINNNAAIRAV
                     FEMKGRPLNHPLIVHIASNWDLHKLVEEVPEYAHQLASKFWPGPLTLVFYCKKDQINP
                     LITGGQSTIAIRCPDHPLTQKLLQKLNIPVVAPSANPFGKISPTTALHVKQSFNDAEL
                     TILDGGRCRVGIESTIVDATNPVSYQILRHGIIDEKTIAETIAIGCSTQKNNIRVPGK
                     LDSHYQPQKTLYYFDQYETLSEFCQKRKGTVYVIANRQPPGVETNLFHPLGMHPEKSA
                     YDLYYQLRLADTSDAICIAIELPPNTSEWQGVRERILKAGFPYSQQ"
     gene            11254..11340
                     /locus_tag="I9760_001499"
     tRNA            11254..11340
                     /locus_tag="I9760_001499"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11288..11290,aa:Leu,seq:cag)
     gene            complement(11345..12196)
                     /gene="psd"
                     /locus_tag="I9760_001500"
     CDS             complement(11345..12196)
                     /gene="psd"
                     /locus_tag="I9760_001500"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842959.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAT5043272.1"
                     /translation="MFRDVLKTLPQYLIPKHGITALAGYFADVKNPRLKNFLIQNFIR
                     KFDVDMSEALIEDPKSYDCFNDFFIRHLKPECRPLSQSDVICPVDGCISEIGKIERGQ
                     LLQAKGKYYSVQELLACDGQLAEQFVQGQFATLYLSPKDYHRVHMPIDAELVSMTYIP
                     GALFSVQPATTRVVPKLFARNERLAIFFKTKIGPMVMVMVGATIVGAIGTSWHGDVKR
                     SKELERFDYSEQFLDKIISQGSEMGYFKLGSTVVLLFANGEKIQWDKELLAGSKIQLG
                     KPMAIIT"
     gene            complement(12289..14904)
                     /locus_tag="I9760_001501"
     CDS             complement(12289..14904)
                     /locus_tag="I9760_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2975 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043273.1"
                     /translation="MGLPKKALKESQLQFLTAGTAVSDSSHQTYKVSFIENGVIKNAF
                     YKKLDPKNHYPELLAKISVAVSLFKRIFQGRRSAEERLVFDDEERLVGTLSISVDGFK
                     GFNFHKESVPQESSAKEQVIPSTRTLIEKSFMEILLGRWFLDDDDGHPHNLSLAGDID
                     FDMFFYWFTIYMKEPRPAIGIPKTRVNLTVRDWEGFPNVKDSKPFHWPTYKNPGQETL
                     PTVLPVQDKLVNLILEKTYPDPGQFEQLAHEPVAQEQKFAAALKILLTYQPEMIRKRL
                     TELFGEMTLNYTSLDETDVALRNQYEKTFPHLCNENTNIKPFVDFIMNLYQMHYDNLY
                     RVVVFYMGCENNGYGVPLPATNSALYHKPSFYKDIVEWARTQNITIFSKDDSSIKFDE
                     DELRRRYHQVWRDAYAPTFRDLLHDSYSLTNKLLQQVSTFHVVLDEVEGKKPTDDTLT
                     NAWELFGTMPELSLEKITPLISVDKDSKLRTALILLVEFTTQFHAVAKTYYQKDRKDL
                     TEEDNLEFSEQLVQLYTNYNLKIRQSLAHTSTLAGEFNRIAVGLKQYTERANFQLHLT
                     TTDEQMKEATVATTPKEILPHTHEDVIRQFNDSLFLWAKNLRPEDLSHHISEIIDKYY
                     APTIELLSKRHRAQPVKEYLQASVNESGENRLAYILSAGEGDTGALNTLLIQHLTPYM
                     LQTYPLLSIRNAVKEGNFDKDLEIFTKAAVDFAKHDRRFIHLYNVEGKSLFFKTMYEW
                     IDELPATKFKGLLESALKDYEGKLWWSTSRRSEVEGYCTKFSQAKIVAMTFLNGKDSS
                     SLNDVLFDKIIAAIQKDINKNKEKLKIPGFRLINCYNAKEHRADYFKEVKNYAEPISH
                     RQETTLNSNVTSLVV"
     gene            complement(15167..16696)
                     /locus_tag="I9760_001502"
     CDS             complement(15167..16696)
                     /locus_tag="I9760_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043274.1"
                     /translation="MSITAQELVKQYKLRLTPAMENDLLSEESRLKKELEAVPFNSEE
                     TLYKSILQMIIVFYEENTLEENRYLLQDHELIKQLSALMWDDIQIKLIPFLIQKNFTL
                     SEIKELLFDDAYYRSLHVLVDFGLTQDIPELLAHQEKREQLKFINTLANDHCRKLCLI
                     FWVKGSLSIKEIQDIVNATSHYPMLAETLIALDKTKTISIKQLKKLALDPKKHQQESI
                     LYHYSEQFKAYNLRKSDLSQLNLDDLDALGKSFKVLKEAGIANDYAYRLVLKNNKTGQ
                     LLRLFLPGLAKIESLSHRKALIELLYIGAQKGVVTQGKALLQIKDSNLLALSRALRER
                     FICVQQMQDLGFKKEIIAFTGEENNINSSRFRHVIMRVEEKCKDIHERLRKSSLDKDK
                     VGNWQRADEKYRQTLYSIAYDGITKSGVDLHIKMKSAEKEILSIVDPEIKSIIHKVLV
                     VIANIIITALTLGFANDLKESATGNYWFFNQSPSGEVIRALNKEVLTTIDSPELITIL
                     P"
BASE COUNT         5273 a         3400 c         3024 g         5176 t
ORIGIN      
        1 gttccagcaa acactgcacc gcttcctaag gcgagtactg ccactgctgt ttttttcaaa
       61 ctaaacatgc cttgtctcca cttgtttatt attgagtttc cattttattg ataactcacc
      121 atttggagtt gtcaatagca cgcatcgtcg cttaggttcg aagcatgcaa cgatatgagt
      181 atctgcagtg attttaaata agtcaagaaa atttattaaa aattccaata ttttcataac
      241 gatataacta aatttgaaca gtatctatat ttttttaaca ttattatcat gtctctttca
      301 aatgaaattt gttgaccaag aagggggttt tcgaggatga aatgccatca tgaaaagata
      361 cttttttaat cacaaaattt atttttttat caatgtcaca acaacacgtc tgttagcagc
      421 tctcccctcg tcagtatcat tcctggctat cggttgcaga gctcctaccc atgttattga
      481 tagttttctt tgaggtactc caagcctaat caaataattt ttcacagact ctgctcgata
      541 ttgtgaaatg gcattattgt aacctttacg gccgctatcg tcagcatgac caataatctt
      601 aatggcatca atttgtacat cagcatccac gtattgggca atccgtcgta attgcacttt
      661 atcagcatca gacaataact tactatccac cccaaaatga aacacgcttt cttttacatc
      721 ctcatagttg aaaggcaaca ggccactcag acaacgttga tattgtgaat acactttttg
      781 aaaccgaata ggtgagagtg taaccgtagt gcgaaatcct tcctctgata aatagttaaa
      841 gcttgcctga tatccttgcg ataaaaacgt caataatttt aaagcgggct gtcgatttaa
      901 aaatagtcca tactccccgg gtttaatata agttcgggtt accagataag atctggttaa
      961 taacggtttc cacactgggg gacttgctac aatttgagca ggtaaaaccc gttgaacttg
     1021 atcccattta cgcagaatga aatgagggga ttgggtagcg tattgctcaa agtagccaat
     1081 accataattt ggaatcgtca atgacaagcc acatcgaaga ggattgcctg tcatacgcca
     1141 attttcttct cccatagggc ttgcgtattg gatagaggga gcagcaaaga cgacagtagt
     1201 aatgaataaa gaactcattg caataatttt tgccttcttg catataaaat gctctgaaat
     1261 cattttttgc tcatccaaat tgcctaattc atgttattct cttttatcgg caataatgca
     1321 ggaaccttta ttatgcaaac tctgataatt aatcgccctg acgattggca tctccatctc
     1381 agggatggag agtttctaac tcataccgtc caggcgtctg cccaacattt tgccagagcg
     1441 ttagtgatgc ccaatctcaa accagcacta accaatttat ctgcaattga gagttataga
     1501 aacagaatca tttcggccat tcccaaacaa aactccttta ttccttatat gactttttat
     1561 ttaaatgagt ccgtcaaacc agaagaatta cagcaagccg cctctctttc ttatatagtt
     1621 ggagcaaaac tttatccagc tggagcgacg acaaattctg aagagggtgc taaatctttg
     1681 aaagcactct accccctttt tgaaacattg caagcgaata atttagtctt gcaaattcat
     1741 ggcgaagtca ctcatagcga tattttcgag cgtgaagcat tatttattga tgaatacctg
     1801 aaacctatcg ccaaacattt ccccaaatta agaatcgtgc tggagcacat atcgactaaa
     1861 actgccgtgg attatgtttc tgaagggcct gctaacctta cagcaacaat aactcctcac
     1921 catttgcttt ataatcgcaa taagctactc gcagggggca ttagacctca ttattattgt
     1981 ttgcctattt taaaacacga aaaagaccaa aaggccttac aaattgcagc gacaagcgga
     2041 aatccaaaat tttttgccgg cactgacagt gcccctcatg ccatcaatgc caaggaaaat
     2101 gcatgtggat gcgccggtat ttattcagct ccatatgcgg tagcgctcta cacgcagatc
     2161 tttgatgaac ttaaccaatt aaaaaaactt aatgattttc ttagtcgatt tggtgcagag
     2221 ttttaccaac tccctattaa tcaacagcaa ttggaactca ttaaatcacc tcaaactatc
     2281 cccaactcaa tgccatttgg atcaactcat gttgtgccta ttgctgctgg agacactata
     2341 aactggagta ttaatggttt ctcgcaataa tattctaagt aacaccatta aaaatcgctt
     2401 tcgtggcttt ttgcctgttg ttgttgatgt agaaactgcg ggaatagacc ctcaaaaaaa
     2461 tgctctcctg gaaatgtgta ttgttttatt ggatatggat gagcaaggct tgttaaaaaa
     2521 ggatagtagt tattttgaac atattttacc tttcccgggt gcagaactgg atcaaaaatc
     2581 actggaattc aaccaaatag atccttacca gcctcttcgt tttgcagtag atgaacaaac
     2641 tgcccttgaa cgattattca aacccatcca tgacttgcta aaaaaaaccc gttgccaaag
     2701 agctgtttta gtagggcata atgcctggtt tgatttgtta ttcatcaaag cggcaatcaa
     2761 aagaaccggg ataaaatcac ctttccatgc cttcacttgc tttgatacag caacattagc
     2821 gggtttgatt tttggagaaa ccgtattggc caaagcagct caagcagccg gattacattt
     2881 tgacagccag gaggcgcatt ctgctattta tgatgcggaa aaaacagctg atttgttttg
     2941 ccttatgata aacaagtggc gaatgctgaa catctgatat cagaaaaaca taaatagcct
     3001 ggattaccta aaacagtaac ccaggagttt gatagaaatt ttcttgtgtt atgcttcaca
     3061 ataaaacaca acaacaaaag aattctattt cctcgccctt atattacaag gactcggaat
     3121 gctcagacaa atattctgca acacctttag gagatgcttt catgccagct tgtccttttt
     3181 gccagccagc aggacacacc tcaccatttt cttcaaagaa ttgaactgca tctatgatac
     3241 gaagaatctc atcaatattt ctacctatcg gcaagtcatt gacgatttgt gaacggacca
     3301 tgccattcgt atcaataaca aaagcgcctc ggaaagccac gccagcaact ggatgctcta
     3361 cgccatagga ttggcaaata ctgtgagtca tgtcagctgc caaagtaaat ctcacaggcc
     3421 ctattccgcc atttttcaca ggcgtattcc tatacgcatt atgggtaaaa tgtgaatcaa
     3481 tagatacagc aaccacctca acattacgtc ttttgaattc ttcaatacgg tgatcaagag
     3541 caattaactc ggaagggcat acaaaggtaa aatccagggg ataaaagaaa actaatccgt
     3601 atttgccttt caaatgttcg tgtaaattga atttatcaac aatttcccca ttgcccatta
     3661 ctgcagccac agtaaaatca ggtgctttac gcccaactaa aacactcatt ctataatctc
     3721 cattagcggt gggtgagtaa atcccacaat tgttattaaa attatgccgc tactgcatca
     3781 cgcaacatag gctctaactc tccttgctga aacatttcgg ctatgatgtc agatcctcca
     3841 attaattccc ctttcacata aagttgagga aaggtaggcc agtcagaaaa ttgtggtaaa
     3901 acttgacgaa tatcaggatt ggcaaggata tcgacatagg caaagtctac tccacaagcc
     3961 tcaatacact gaactgcacg agcggaaaac ccgcattgag gcatcttggg agtgcctttc
     4021 atgtagagca ttatcgcatt ttcagcaatt tgctttttaa tcttttctaa agtatccact
     4081 atgcaccttt tataaaataa aaaacccaat tatgtcgaaa accaaacaag gtcgcaacta
     4141 atgattctat tgtgataatc ctatcaatct ttttcttata ttgatatttt accctacctt
     4201 aacgatcaat aaaataccaa tttagtacta aatgtgccaa aaatccccac cttgacgaga
     4261 attaaaatcc actattttgt aaaagattat tataccagca gaaatgtatc catggccaag
     4321 actataaaaa tattgaatcg ctcagtaatg gcacattgaa tatcacactt atcctgataa
     4381 actaaagact tttaatcctg gagattgata tgacttttac cctgccgcaa ttaccttatg
     4441 cattggatgc actggctcct catgtttcca aagaaacctt ggaataccat tatggtaaac
     4501 accataacac ttacgtcacc aacttaaata aattaattcc cggaacagaa tttgaatcca
     4561 tgactctgga agagatcatc atgaaagcta aaggcggtat ttttaacaac gccgctcagg
     4621 tttggaatca caccttttat tggcacagca tgagccctaa tggtggaggt gagccaaaag
     4681 gcaggctggc ggaagccatt aataaaagct ttggttcttt tgcagcattc aaagaacaat
     4741 tttcacaaac agctgctaca acattcggtt ccgggtgggc atggctagtc caggatcagt
     4801 caggggcttt aaaaattatc aacacaagca atgctggaac accaatgact gaaggattaa
     4861 atgccttatt aacttgtgac gtatgggagc acgcttatta catcgattat agaaataggc
     4921 gcccggatta tattgaagcc ttttggtcat tagttaattg ggattttgcg agcagtaatc
     4981 tgaaataaaa taacaagctg gatttgttgc aatccctacc ggataatttt atgtttatgg
     5041 agacatcatg agtttaatta caagttacag cccaatgcct gtaaccttca cgcacggaga
     5101 aggcgtgtgg ttgtatgatg agcaggggaa aaaatacctc gatggcttaa gcgggatagc
     5161 agtttgtgga ttaggtcatg cacaccctga tgtcacccgt accatacagg agcaggcagc
     5221 caggctaata catacttcta atgtctttca tattaaagaa caagaattat tagccgccaa
     5281 actcacttca atggcacgaa tggagcaggt tttttttgct aattctggag cagaagctaa
     5341 tgaagcagcc attaaactta ctcgtctgtt tggtcataaa aagggaattg aaacgccttc
     5401 tattattgta atggaaaaag catttcatgg tcgcactatg gccaccttaa ctgcatcagg
     5461 cagtcgtaaa gttcaagctg gttttgaacc cttggtccct gggtttattc gtgctccctt
     5521 caatgatctt gatgccattc atactattgc ggccaacaga gaggatgtgg ttgcagttat
     5581 ggtagaaccg attcagggcg aggggggaat ttatcccgca gaggaaggtt atctgcgtgc
     5641 attggctaac ctttgcgagc agaatgactg gatgttaatt ttggatgaaa tacaaacagg
     5701 aaatggcaga acaggtaagc tttattcttg tatgcattac gacatacaac ctgacattct
     5761 tactacggca aaaggacttg gaaacggtat gccaattggg gcttgtttaa tgagcaagcg
     5821 agctaaagat ttatttaaac ctggaaatca tggctcgact tttggtggca accctttagc
     5881 ttgtgcaacg gctttgactg ttctggaggt cattgaacga gataagctat gtgagcgcgt
     5941 cactaaaaac agtgctttgt taatggataa attaattagc aacctgggag agcatcccca
     6001 tgtaaaagct atacgtggta aaggttatat gataggaata gagttggaca gacctgcaaa
     6061 tgacatgcgc gtcctgggtt tggaaaacca agttttgttc aatattacag ctgaaacagt
     6121 agtaagatta ttacctccac ttattatcag tgaagatgaa attgatgaat tggtcaatcg
     6181 aataactcgt actattaacc agttcaccaa tcagtagtga gaagacaact ttctctcaat
     6241 tgaatagaaa ttgttttctc cctggctgtc tcgcagtatg ctaaattaat ctggtatctg
     6301 acaacatgct ccctggattg tccgggagca tgtgtgtgag ttacatcaaa acacaaagct
     6361 ttgccccttg gcaacaaaac ctaagaaacc atcctttttg gcattaggca atttccctgg
     6421 attataatga tatagccaca tttttatctt taaatgagag ggaagtttaa cgagctctga
     6481 atagtgcgca tgtaccccgc ttttagattc tgtagtttca caatcatgaa aaataatatc
     6541 tgattcctca taaaaggaaa ttaactgtac cggacaactt tgtgtgtctg atgtaaaaag
     6601 gatgcgtgat ttattatagc taaatatcaa accaaaactt ggcatcaatt catattcact
     6661 atagtaatgg actgtttgta ccaatttaaa ctttattgat cgccaaacaa aaccttcata
     6721 ttgttttacc gccttaacat caaaaaaagc gctaagattt gctctctcat gaggcaaggt
     6781 gcttaacccg ccggataaac ttttatccca taattcgtta ataattttat cagaagcgat
     6841 taaacaaggt ttgtgcgaga gattaggatc aaaataggtt attagagcga accattccaa
     6901 tccaccgcaa tgatcataat gcaagtggct aatataaata ttttttatat ctttataact
     6961 caatccttga tctcgtaatg agtatttgat atctgttcct gcatcgatta ataaagtatc
     7021 gttttctagc tctaaaagca cattggattg ataattttct ggcccaatag caaaggatga
     7081 tccggaacct aaaaaagtaa tttttagagt cattgtaccc cctgccgtca ctcacgaaaa
     7141 tgtcccgacc ttaaacctgc tatctttaag gtactaatcc ttgctacatg ataaaatcga
     7201 actgttaatt taagactatg tgatgattat tgaattgtca aaaacaggca ggagtattgt
     7261 caaaatgatt acattctatt ttttaagtca tttaaatact gattaatgtt tattgcctca
     7321 ttagtaaaga gccatttaac ccctctattt aattctctat acagactgtt tttggaatca
     7381 acaaatccca cgccaaaatg ttgattggca tcttgtaaat attttaatgc cctattagtc
     7441 aataataaat aattacccag tacgcctcca tacccctcct tgatgcttaa ttcacaaaat
     7501 ttcttaacgt tattatgcac tgcaacaagc aataaagatt gcctaggaaa agaacacaat
     7561 tttttaaagg tagtttcctg taaagtcaat aaatgaaaat ctttacatgg cgttaaatgt
     7621 tgcagggttt gcactaatat ctcttcctcc tcaatagctg tttttaattc aataaataaa
     7681 tgcatggaat ttccatactg actcactact tcagagagcg aggggattct gcactccaat
     7741 gcccgtaact cactaaaatt cagatcagca atagcaatgt ccttgttcca taatcgattt
     7801 aaggtaggat catgattaac aacgaacacc ttgtccttag tgaggtgtac atccagttca
     7861 attccccaac atccggtctc ttttgcgttc tgaaatgctt ccagagtgtt ttcaattatc
     7921 cctcttgcat tattatgagc tcctctatgc gcgattatta acgcaccgtt gatattttcg
     7981 aattccggtg tgtgcctggg aattacagca aaatactcat caattatttt ttctatgcat
     8041 ttgagtaaca aaaccaatta tcctaatagt ccaattttaa tcattttatg ccggcataaa
     8101 ataaaaggca aaatacgcta ttagcacaaa atcacaaaaa tcgactaaac tagaaactat
     8161 gtgagaatga caatacaaca ataaattagc atttataaaa tttgtaaata acttccatct
     8221 taaaatcaat gagttgaatt gaagttattt agaagtcctg atttaaattt ctcggttaca
     8281 aatattcaca aggagtgttc taatggcaat caaagtaaca aaaaggggaa tggacatact
     8341 aagagacccc atactaaata aaggtaccgc atttagcctc caggaaagag atgaatttgc
     8401 cttgcatggt ttaataccaa caacagtgga aacccttgaa caacaagtag ttcgctgcct
     8461 tgacgcttat agtgccaaag aggacccttt ggagaaacac atttacttac gtgccttgca
     8521 ggaccgaaat gaagtacttt tttatcgttt tataattgat aatctggttc atattttgcc
     8581 cattatatac acaccggttg tcggccaagc ctgtgaaatg ttttcccaca tttatcggca
     8641 accaagagga gtatttctaa gttatccaga gcgagacaaa ctggacagca tcatacaaaa
     8701 cattgcgtcg actcgttcaa ccaaagtaat tgttgtcact gatggcgaac ggatcttggg
     8761 gttgggagat caaggagcag gtggattggg aattcctata ggcaaattgt ctctttatac
     8821 cagttgcggt ggtatacatc cttctaacac tttacctatt attctggatg taggaaccaa
     8881 taataaggag cgtctggacg atccggaata tattgggtgg agacacgctc ggatttcagg
     8941 caaagagtat gatgattttg ttgatcaatt tgtgcaaagt ataaaacgcc atatgcctca
     9001 tgtgctatta caattcgaag attttgcaca acaacatgcc tatcctttat tggagcgata
     9061 taaaaatcaa ctttgtactt ttaatgatga tattcaagga actgcttctg ttgctgtagc
     9121 cgccatacta gcagcaactc gtgttactaa tactccttta aaggaacatc gagttgcttt
     9181 attaggtgca ggatcagcgg gttgtgggat cagtgagcag ctggtgcatg ccatgatgaa
     9241 ccagggactt tctgaagaag aagccagatc acgtttttat ttagttgaca ggtatggact
     9301 tttacatgat gagatgacag atctattgcc atttcaaaaa ggatttgtcc gttcttctac
     9361 cagtctccaa aattggaaat tagaaaaaaa aggagaaatc acgttaacag acgttattaa
     9421 caatgcccaa ccgactatac tcctgggggt gtctggccaa cctaaccaat tcaaggaagc
     9481 aatgatcaag accatgctaa gctattgtga acgccccata atttttcctt tatcaaatcc
     9541 tacgtctcgc gcagaagcaa tcccgcaaga tctgttaaat tggacagctg gtaaagcttt
     9601 aattgcaaca ggcagtccct ttgagccagt tgtaattaat ggtcataaaa ttgagatagc
     9661 tcaatgcaat aattcttaca ttttcccggg agttggtctt ggagtagttg caggacaagc
     9721 caaaagagtg actgatttaa tgatgatggc agcagcagtt gcattaagtg agctagcacc
     9781 tgcaatcaga accggagaag gacgcttact cccagaatta aattccatta gagaagtcag
     9841 tcaacatata gcgagagcgg taattttaca gggtataaaa gagggtcata tagagccaat
     9901 gaacaataat aaaatcgatg attctataaa aagaacgatg tggactccac aatatgaacc
     9961 atatgttcta taataaaccg atgtagaaat tgctggaatt taagttttaa ttttttagaa
    10021 agactaaaat ttattagttt atcgcaatag aatttacagc atagggttga gggaaaatca
    10081 ggttattgat cctacctgat tccctccagt ttatttatag aagcttattc ttattattaa
    10141 attgagctta aaaacagata gaaagaccct gaaacataac tattttttat cgagcccctt
    10201 atatgtccat aattactacc aatctcaatc gggcaattca tgatttacag caaggaaaac
    10261 cagtagctat accgactgaa acggtttacg gattggcagc accaataaac aataatgctg
    10321 ccatcagagc tgtgtttgaa atgaaaggca gacctttaaa tcatcctttg attgttcata
    10381 ttgcttccaa ttgggatctg cataagctcg tggaagaggt acctgagtat gcgcatcaat
    10441 tagctagcaa gttctggcct ggaccattga ccctggtatt ctattgtaaa aaagatcaaa
    10501 tcaatccgct cattaccggt ggtcaatcta ccatagcgat aagatgcccg gatcatccct
    10561 tgacacaaaa attgttacaa aaattaaata ttcctgtggt ggcaccctct gccaatcctt
    10621 ttggaaaaat tagcccgaca accgctttgc acgttaaaca atcatttaat gatgccgagt
    10681 taactatcct ggacggagga cgctgtcgtg tgggtattga atcaaccatt gtagatgcta
    10741 ccaatcctgt ttcttatcaa attttacgcc atggaataat agacgaaaaa actattgctg
    10801 aaacgattgc cataggttgc tcgactcaaa aaaataatat tcgtgtccct ggtaaattgg
    10861 atagccatta tcaaccacaa aaaactttat attattttga tcaatatgaa accctttccg
    10921 aattttgtca aaaacgcaaa gggactgttt atgtcatagc gaatagacaa ccccctggtg
    10981 tcgagactaa tctctttcat ccactgggaa tgcatcctga aaaatcggcc tatgatttgt
    11041 attatcaact aagattagct gatacatccg atgccatttg tatcgctatc gagctaccac
    11101 ccaatacgag tgaatggcag ggagtacgag aaagaatatt aaaagccggg ttcccctatt
    11161 cgcagcaata aatctcaagt aatttatact tttttgattt cgcgcaattc tgtattgaca
    11221 gaacactata atttgcgtaa cttaacgccc tatgccgagg tggtggaatt ggtagacacg
    11281 ctagcttcag gtgctagtgg gggcaacccc gtggaggttc gagtcctctt ctcggtacca
    11341 ttaattaagt aataattgcc attggtttac ccaactgaat cttgctacca gccaataatt
    11401 ctttatccca ttgtattttt tctccatttg caaacaataa aacgaccgta gatcccaatt
    11461 taaaataacc catttcactt ccctgagata taatcttgtc caaaaactgc tcactataat
    11521 caaagcgttc taattctttt gatcttttga catcaccatg ccaactggtt ccaattgcac
    11581 ctacaatagt agccccaacc atcaccatca ccatcggacc aattttcgtt ttaaaaaaaa
    11641 tggcaagcct ttcatttcgt gcgaacaatt taggaacaac acgtgtagtt gctggttgaa
    11701 cggaaaacaa agcaccagga atataagtca ttgaaacgag ttctgcatct attggcatat
    11761 gtaccctgtg gtaatctttc ggcgataagt atagcgttgc gaattgtccc tgcacaaatt
    11821 gttcggccaa ttgcccatca caggctaaaa gctcttgtac agaataatat ttgcctttag
    11881 cctgcaataa ctgtcctcgt tcaatcttgc ctatttcgct gatacaacca tctactggac
    11941 aaatcacatc tgactgagaa agaggacggc attccggctt taaatgacga ataaagaaat
    12001 cattaaaaca gtcataagac ttaggatctt caataagcgc ctcactcatg tcgacatcaa
    12061 acttacgaat aaagttttga attaggaaat tcttgagacg agggttctta acgtcagcaa
    12121 aataccctgc taaagcagtg ataccgtgtt tcggaataag gtattggggt agtgtcttca
    12181 gtacatcacg aaacatactt tttactcaaa actgaaaaaa gagaataata acctaaagct
    12241 tattgtattg tcacaataag acgagtaaat ctccatgcca gagaaaattt agaccaccaa
    12301 gctcgtaaca tttgaattca aagtagtttc ttgcctatga gatataggtt ctgcgtaatt
    12361 tttgacctct ttaaaataat cagccctgtg ttctttggca ttgtagcaat taattagcct
    12421 gaatcctggt atctttaatt tttctttgtt tttgtttatg tctttttgta tagccgcgat
    12481 gattttatca aataaaacat cattcagaga ggaggaatct ttcccgttta aaaaggtcat
    12541 agcaacaatt tttgcttgtg agaattttgt acaataacct tccacctccg atctccttga
    12601 agtggaccac cataatttac cttcgtaatc ttttaatgca gactcaagca accccttaaa
    12661 cttagtcgcg gggagctcat caatccactc atacattgtt ttgaaaaata gagacttacc
    12721 ctccacattg tataaatgaa taaaacgcct gtcatgtttg gcgaaatcta cagctgcttt
    12781 ggtaaaaatt tccaaatcct tatcaaaatt accttcttta actgcattac ggatgctcaa
    12841 caaaggataa gtttgtagca tataaggcgt gaggtgttga ataagcaaag tatttaaagc
    12901 acctgtatca ccctcccctg cgcttaaaat ataagccaat ctgttttcac cactttcatt
    12961 tacgctggct tgtaaatatt ctttaacagg ttgtgctcta tgacgctttg ataacagttc
    13021 tatagttggg gcataatatt tatcaattat ttcagagata tgatgagata aatcttcagg
    13081 tcttaagttc ttggcccaaa ggaataaaga atcattaaac tgcctaatga cgtcttcatg
    13141 cgtatgcggc aatatttctt tgggggtcgt ggctacagtt gcctccttca tctgctcatc
    13201 tgttgtcgtt aaatgtaatt ggaaattggc ccgctctgtg tattgtttta gcccaacggc
    13261 tatgcgatta aattcaccgg caagagtgct tgtatgtgcc aaactttgac gaattttcag
    13321 gttataattc gtatacaact gtaccaattg ctcggagaac tcaagattgt cctcctccgt
    13381 taaatccttt cggtctttct gataataagt cttagctact gcatgaaatt gagttgtaaa
    13441 ttcaacaagc aatattaacg ctgttcttag tttgctgtct ttatctacac taattaatgg
    13501 ggtaattttc tcgagtgata actcaggcat ggtgccaaac aactcccagg cattggttaa
    13561 ggtgtcatcg gttggctttt tgccttctac ttcatccaga acaacgtgaa atgtagacac
    13621 ttgctgcaat agtttattag tcaggctata ggagtcatgc aacagatctc tgaatgtagg
    13681 cgcataagca tctcgccaca cctgatgata tcttcttcgc aactcatctt catcaaattt
    13741 gatgctgctg tcgtccttgc taaaaatcgt aatattttga gtccttgccc attcgactat
    13801 gtctttatag aaagaaggtt tgtggtagag cgctgaattc gttgcaggca acggaactcc
    13861 gtacccatta ttttcacacc ccatataaaa aaccactact cgatacaaat tatcataatg
    13921 catttgatat aaattcatta tgaaatcgac gaatggtttt atattggtgt tttcattgca
    13981 taaatgagga aatgttttct cgtactgatt gcgcagggcg acatccgttt catctagaga
    14041 agtgtagttt agcgtcatct caccaaaaag ctcggtcaaa cgctttctta tcatctccgg
    14101 ctgataagtt aacaatattt tcaacgcggc agcaaatttt tgctcctgag ccactggctc
    14161 atgagcgagt tgctcaaatt gaccaggatc cgggtacgtc ttttcaagaa ttaaattaac
    14221 cagcttatct tgaacaggca aaaccgttgg caaagtttcc tgccccggat ttttatatgt
    14281 tggccaatga aaaggctttg agtctttgac attaggaaat ccttcccaat cgcgcacagt
    14341 caaattaacc cttgttttag gtatacctat agctggcctg ggctctttca tatagatggt
    14401 aaaccaataa aaaaacatat caaaatcaat atccccggcc aaacttagat tatgcgggtg
    14461 accatcatca tcatcaagaa accaccttcc cagaaggatt tccatgaaac ttttttcaat
    14521 taaggttctt gtgctgggaa tcacctgttc tttggcggaa gactcctgag gcactgactc
    14581 tttatggaaa ttaaaccctt taaatccgtc aacactgata gataaagtac ctaccagcct
    14641 ctcttcatca tcaaatacaa gacgttcttc tgccgacctt cttccctgaa aaattctttt
    14701 aaataaagag acagcgacac ttattttcgc taaaagttcc ggataatgat ttttaggatc
    14761 cagtttttta taaaaggcat ttttaataac gccattttca ataaaactga ccttgtatgt
    14821 ttgatgagag ctatccgaaa ccgcagtccc cgctgttaaa aactgcagtt ggctctcttt
    14881 cagtgctttt ttgggcaaac ccatatatca aatcctttaa atcagatcaa tccatcctat
    14941 gccccatatt aatcagaaat agtcaagaag ttcaagtata attttcatac aaaattacac
    15001 ttgactagaa acaaactaga catctggcac acataatgag ttataaatga aaatttgctt
    15061 gtttatttgt catgatgacg aactcgaaat catcatttta attaaacatt cagggttgtc
    15121 ttatattgcc caaagcaata agggtttgtt aacagaagcc ctatgattag ggcaaaattg
    15181 taattaactc cggagaatca atagtagtta atacttcttt atttaaggct cttatgactt
    15241 ctccagatgg actttggtta aaaaaccaat aattgccagt ggcactttct tttaaatcgt
    15301 tagcaaaacc caaggtcaat gccgtaataa taatattagc tataacgact aaaactttat
    15361 gtattattga ctttatttca ggatcaacaa tactcaatat ttccttttcc gcacttttca
    15421 ttttaatgtg cagatcaaca cctgattttg ttatgccatc ataggcaatg ctgtagagtg
    15481 tttgccggta tttttcatca gcacgctgcc aatttccaac tttatcttta tcaagagatg
    15541 attttcgtaa tctttcatgt atatccttgc atttttcttc tacccgcata atgacatgcc
    15601 tgaaacggct ggagttgata ttgttttctt cgcctgtgaa tgcaatgatc tctttcttaa
    15661 atcctaaatc ttgcatctgc tggacacaaa taaatcgttc gcgcaacgct cgtgacaaag
    15721 ccaataaatt gctgtcttta atttgtaaaa gcgcctttcc ctgagtaaca accccttttt
    15781 gggctcctat atataaaagt tcaattaaag cttttctatg agagagcgat tcgattttag
    15841 caagccctgg caaaaacaag cgaagcaatt gccctgtttt attatttttt agtaccaaac
    15901 gataagcata atcattggca atccccgctt ctttcaagac tttaaaagat tttcctaaag
    15961 catccagatc atccaaattt aattgggata aatcggattt acgcaaattg taggctttaa
    16021 attgttcaga gtaatgatat aaaatagatt cctgttgatg ttttttggga tccagagcca
    16081 gtttttttag ctgttttata gaaatggttt tggttttatc taatgctatc aatgtttcag
    16141 ccagcattgg gtaatggctg gtagcattaa caatgtcttg tatttctttg atcgataaac
    16201 tccccttaac ccaaaatata agacatagtt ttctgcaatg atcatttgct aaagtattaa
    16261 tgaattttag ttgctctctt ttttcctgat gggctaataa ttcagggata tcttgagtta
    16321 aaccaaaatc gacaaggaca tgaagcgaac gataatacgc atcgtcaaat aagagctctt
    16381 taatctcact taaagtgaaa tttttctgaa ttagaaaagg aattaacttg atttggatat
    16441 catcccacat caatgcgctt aattgcttta ttaactcatg atcttgcaat aaataacgat
    16501 tttcttccaa agtattttct tcataaaata caataatcat ttgcaaaata cttttataca
    16561 gagtttcctc agagttaaaa ggaaccgctt caagctcctt ctttaatctg ctttcttccg
    16621 acaataaatc gttttccatg gcgggagtca aacgaagctt gtattgtttt accaactcct
    16681 gggctgtaat actcattgga agttaacctc ttgttcattt actaattaac cctatttcat
    16741 ggagataggg taataaagat tattttttgg atcaaaattt ggttattttg aacgatacat
    16801 aaatagtatc aaaactacaa aatgtaaata ttataaccaa ttttgcagca aatggggatt
    16861 ttttttaaaa ata
//
LOCUS       DACTJE010000059        16746 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.059, whole genome shotgun sequence.
ACCESSION   DACTJE010000059 DACTJE010000000
VERSION     DACTJE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16746
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.059"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            190..11532
                     /locus_tag="I9760_001503"
     CDS             190..11532
                     /locus_tag="I9760_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAT5043275.1"
                     /translation="MVNVTMKNKTKNLLNEPIAIIGMNCQFPGIDSDIEDISAFYDML
                     MKGQTPIKDVPENRWNIEEYYSADRKKADKIISKKGGFLDNTHLFDAAYFKISSAEAK
                     QIDPQQRLFLEVAIRALNDANIPLDSLKDSNSGVYCGISTHEYSQLNYKDHIKFNAYT
                     PIGIANSAAAGRLCHFLNLKGPSMAVDTACSSSFTALYLAATALRNQQCDMAIVGGVH
                     LSLCPESFIGLTKANMLSATGQCSSFDSKADGFVRSEGCGVVIVKRLTDAIRDNNKIY
                     ALIKSMVINQNGDGTGLAAPSTNAQIAMHQAALEHAHLTAGEIDYIETHGTGTTVGDP
                     VEFNAIQSIHQGHHSPQKPLIVGALKSNIGHTISSSGIASLIKVLCSLQNEAIPPNLH
                     YSNPNSLIDPESIPALLPVEAIPFLKLKNKKRYVQVSNFSFTGTNVSVILEEAPELES
                     HESKQDDNEPKCFVASANSELSLKQLMSRYEHYLKNSSSTLGDICHTLINCRDHYKFR
                     CAIIVNDKKELIKKIESKDYEIKKVVLQNNPRTVLNDAKAIYEAYLSGINISTDDKNT
                     AYNKVDLPLYCFDRKPYWHEVRKQVKNDHWLDSLYQQPIEQQVAIIKTELASQIKDLL
                     KKDNIDPHQDLETLGLTTSLLESLDQIFQDMFSPRYKVPSLQSLRYLTLDKLARHLQK
                     MIMPPVVYRQPSINVLNVEPIAIIGMSCRFPKAANVDEFLSLLEKGESGMIDIPLERW
                     DNEKFYDSDVDALGKLYIKQLGFIDHVNHFDAEFFNISPREAKLMSPQLRVFMETSYH
                     ALEDANLSLDAVKDTNTGVFVGVGTNEYPRVLAYQGVTLEDLNIYFATGNVLNALAGR
                     VAYSFDFHGPIQAIDTACSSSMTAIHNACLSLQSGDCNMALAGGVNIILSPDSNITLS
                     KARMLSPDSRCKTFSEDADGYGRSEGCGVVVLKRLSDAVKDNDNILAVIKGTSINSDG
                     KSGGFTVPNGAAQEEVILSALAKANLSPGDIDFVEAHGTGTPLADPIEVNTLTKIFSE
                     HHNKENPLYISSVKTNIGHSESASGVASVIKTVLSLKTKTIFKHLNFKKLNPAIQLIN
                     TVIPLDKTDWTKKQGLRCAGVSSFGFSGANAHAILQEAPEDKKEARTLPEESLLVLSA
                     KSKTSLELLLASYQNYLSNTREEFADICYTAATCRSHFMYRVAIKAQNAAQAANIIKS
                     NGYTICHITKEKEWTQQPDTLAELQTAYLDGFPINWSEFYRSLGHHFAKVKLPLYEFE
                     RIEHWFGDKNKLKDAPLPKDWCFQIQWQNEPCDKSNRKLRGNRWLLIGPRHLADGFKA
                     QGLEIVYEDEEYSRDKLEGIIFAESLHLAPPDIEANIDFQKKTLKKLLNLIKELDNQS
                     IDLQLLILTSNGIAELANDKHPVNISNSPLIGFCKTLALELPQFKTILIDMDKIEEDK
                     YRTQVIEEINYNHGSRYEHIVAYRGGERLASRLKKTPLEDRRRLLHGVKGRYLIAGGC
                     GGLGLVTAQSLLSSGARELILTSRNIDKPRIKEAIYKIKSHYPGANIRVVSLDITDRE
                     SLKNLLSELNADGQLKGIIHAAGAAIKAPLLEHQDEDVDYLFSAKVKGGWYLHELSLD
                     CNLDFFVVYSSVSSVFGSNKESVYSGTNSFLDALIAERQRMGLPGTAVQWGPWGEVGM
                     AQKRSRDEGLKQALISNAQGHVIIKCLINDEFKHATIISPEYLKFMLDFVPKPLPGFY
                     GQLDEELEKIPQQHHQSPAKNLSPWLNQYLEISEEERFKACKAMVSDICTGILELTEN
                     EDLNEDEGFFELGFDSLMITELASELKKKLEPFLKVTVTIGFNYPSISKLAKYIESEL
                     DKNLVKIQSPKSTPKPIDDGIAIIGMSCLLPNAPDIAAFEKLLEEGLSGIKDIPNDRW
                     DNNQYYDPDMEAPGKSYVTKLGLIENIKYFDAAFFGISPREAKLMEPQQRVFLECCYK
                     ALENANYPSESLRGSLTGVFAGVGPNEYYAQLEKSGFSNEELSAYSITGNVLNLIPGR
                     VAYTFDFKGPSISVDTACSSSLVAIHYACQSLKNKEIDYALAGGVNILLMPESNVTLC
                     KAKALSPDGQCKTFDERADGYVRGEGCGVVLLKRLSDALRDNDHILAVIKGSAVNNDG
                     KTAGLTVPNGKSQEEVMKKALGQTELSRTDISYIEAHGTGTPLGDPIEVDAINKVYGH
                     QRNKENPLYLGTVKTNIGHLESASGVAGVIKTVISLQRKTIYKNLNFNKLNPNIKLDG
                     TQLALEKTNWKTNTKLNYAAVNAFGFSGTNAHVILAEFPKEEKPKTSKPSQKHLLVIS
                     AHSKTALDNLTQRYQQYLETTEHHFGDICFTAATCRDHYHYRLALTAENAIEASRMLG
                     AGQFALSYHENITLDLQNDRALNSLLMDYLQGKPVDWKSYYQSLKGEFTKVLLPNYTF
                     DRSEYWLDKKNNDSMATHKGEQHPLLGQMLAMPGNEYLFHQKLDLENLAYIKQHSVFE
                     KVIFPATAYIESGLAAAKSIFQSSAFQIEKFNIERPLHPKQGQEFQLQVKPKMDGQYK
                     INLFAKQENDWQTFCEMEIHAKPTAARESIHIDVLKSSFGDKVELSDIYEQFQKSSLF
                     YGDEFRVLQEGYVRTNSVLAKVTLTKASQGQDYYYHPVLLDGAMQSILLLSVKNDENK
                     NFNAENVTYIPYAFIRMTVFQEAPRSLWVHLTKQNTEDASDLCVNMNLYEQSGLLIAQ
                     IAGLKLRRVTRSHFVSYDAALNHLYYTEWSPVPLKTLPQQKIPDFLAITKNPGKAKEI
                     LDGLNYHLVERIDDCTNIENSNIVFLYEQGQFHDLFHCCQILFRLRPKRFILVTEHAY
                     AIQDHDQVNPYHTMACSFWKSFRNEYEPNKNYTIDLDSNSRLATALMYLFNTDTHDTQ
                     IAVRESLYLPRLKKKQLVSNSASERLIDGNATYLITGGTGGLAKLLMEYLMRRGARHI
                     AITSRAHYPDDIKALIDKAKQNQIDIKHYMVDAGNYQKMEQVIGEIQQSPNPLKGVFH
                     LAGLIQDGLIVNLNDEAMQKVISAKMDGALILHQLTQNIPLEWFVMFSSSASLLGARG
                     QANYAAANGFLDGLAHLRRQQGLPAISINWGPFHTTGMAANLTHTLQHYGFLPLDKDN
                     IDILDVLLPSQLPQISPCPMNWDVYCKHTPKQTELSALVKAKPLPDQSFLNALRQHSK
                     EESISILSQALREIAADVLALDDSEHISNHQDLFSTGLDSLMSMDIRNRIYDKLQCQT
                     LSLPIEYFINTPTINKIARHISDELDHVFDSHSDYHPTENPSEREIALCDFQYVFWVL
                     NKLDYHFNIGTQIQLHGRINKDYVQRAFEFVVNQNSVFWIHFNKDKPTQKLHKVGQFE
                     LIYQDISLNHETKALNHEFQNNILRAIPLTKQPLIRVYLYKINNELHELHIVIPHIIV
                     DDASCEMVFCQFKNNYEKLTLGKELVSNPEKGSYFNYVKQNNNHYQKNLKNKIDFWQN
                     YNKGFKMLSFGNRYHLSDTSKQTKHLFHYPIDPQHAEQFINWHKTQNINVSTGLIAAC
                     QIVFYKLSRQNKIPIILIHSGREGGEYKSTLGLFSEYKRINLTLNENSQFIDCIHSIE
                     EQLLKTAPYQKCSHLIKDSGLKGSSLSFGQYLTLALNKMFMLKHFKESKLHSIIIDYY
                     LKYLSRLLSFKKNISMKQRLNQLFKLDMSLQKPERLRVLVSVTPSFFTKERPDMSFAN
                     LDYSYPNHFGCMDRPIGNRTLWIYFSKNQDGEYQISINAPLTIECKNKIGSGLSQFIT
                     TFVENNNCNIAELIQAMEDSSIAS"
     gene            complement(11535..11936)
                     /locus_tag="I9760_001504"
     CDS             complement(11535..11936)
                     /locus_tag="I9760_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAT5043276.1"
                     /translation="MKSLRMAGVLFLFLFSFVSIHVYAAPNTGASNAEMKDVCQLAGG
                     TFTSTIEGNWACCWDNWGCYGCVQGVCKMKCHTQRCRRANGQSAVLQPGEVKIKGKFG
                     TDIVVPKVSTQPSSSNPKQIKNNNTTVNQAQ"
     gene            complement(12166..13119)
                     /locus_tag="I9760_001505"
     CDS             complement(12166..13119)
                     /locus_tag="I9760_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAT5043277.1"
                     /translation="MTSISEWWMWLGFLAFVLIMLFVDLFVFGGGKAHRVSTKEALSW
                     TILWFTLAIIFNFLLWWYLVENFTFSVANEKALEFFTGYLIEKSLSFDNIFVFLILFE
                     YFAIPAKYQHRVLIYGVIGAIVMRLILILLGIWIVNQFHWILYIFGFFLMITGIKMFI
                     FADKKPELAKNPILHWMRSHLRITQELHGERFFLRKNQLVYVTPLFMVLVLIEISDLI
                     FAVDSIPAIFAITNDPFIVFTSNIFAILGLRALYFLLVNMHKRFYFLKYGLAFILVFV
                     GFKMLIAPWFKIPIFIALGIVMATLVFCILFSIWHRKNEVT"
     gene            complement(13234..15096)
                     /locus_tag="I9760_001506"
     CDS             complement(13234..15096)
                     /locus_tag="I9760_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmL"
                     /protein_id="HAT5043278.1"
                     /translation="MPKHLFYGVFLILLSMKAYSQDAVSRDIAKRTESIEKKGIVYHN
                     VIDCDYKISPEISHIDSNIILNWAEYAAILSFNFNFSSIEDQLNQLHSCYTEKGWGQM
                     QNALHKSNNIETIKAEKLKVKGYKDGEAQIIEALDNRWKIILPLKVVYENDKKRVTHF
                     LNAYLTIGWKNKDSLGIMQMIAIPRLAPLAQKVTPADEVIKSMGIILAERKKASLDSA
                     RKITDSFFVFLFSNHGECSKGNLALSNRASQRAAIIQPMPNLNPTHNLVRMQSFDMPE
                     SAKTPYLALSQQKSLVNDSDSNKSTLSDFGNKSRKLYPNWLSQIGLHSSKLQLNSLIT
                     RINPLPLWRAFSQYQQSIRDKSDNIEAINKQKPILSAQIEGEPQFIEVKENQWDIKLP
                     IKIVYQKDDERITQRLDVNLALDQKMINHLTVKNKSLDSNRVLQSLKSILASSSLQLS
                     PQLFLGSQNIQLKQPEQSKPKETVSCHYKIPEETIKTDKNIILQWAEHAATQSFAFDF
                     TSIESQLNKLQSCYTENGWIEFNSAMMKSGNIEAIKMQRLTMTSEVNGVAEFIETREN
                     HWIIALPLKVTYKNDTMRVSQLLNIQLTVGRKTNGEFGVIQLIATLDPSSPPPS"
     gene            15433..15933
                     /locus_tag="I9760_001507"
     CDS             15433..15933
                     /locus_tag="I9760_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HAT5043279.1"
                     /translation="MNTTSLSLTPLLRHSVGFERFNDLFESMRNADDSTYAYPAYDIE
                     KHGEDSYTITMAVPGFQESDLNIMVQNDQLTVSGRIQEKETKESEYLHRSIVTRAFEQ
                     TFRLADHMQVTGAEIKNGLLSIGLIREIPEEAKPRIIPIKSISDQESNKKGKTLEPES
                     KKKISN"
     gene            16087..16638
                     /locus_tag="I9760_001508"
     CDS             16087..16638
                     /locus_tag="I9760_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAT5043280.1"
                     /translation="MDIKKLAPWNWFKEEEAQNKSVPLSTTGQSCISPLSQLHQEIDR
                     IFDNVLRSVGISNLDIEKTPSNSAQNLLLKPCIDIAATDKEYTITVEVPGVEEDHIKL
                     ELSDNTLIIKGEKKHESEKKDKDIYRIERAYGSFQRVLSLPEDANQEDIKAQIKNGVL
                     TITMPRKEVSKPKGKLIDIKKAS"
BASE COUNT         5500 a         3357 c         3126 g         4763 t
ORIGIN      
        1 ctatcaggga gcggatatgg tattcccata caaaatcttg ctgaattctg gctatactta
       61 aggtaaatag accttccagg ttaatttgtt tatactcaaa ttctctctct gttatggttg
      121 tttttattat aactctttgc cagacaaata atttttgtgc gtagccaggc actttcagga
      181 gcattgtaag tggtaaacgt aactatgaaa aacaaaacca aaaacctttt gaatgaaccc
      241 attgccatca ttgggatgaa ttgtcaattt cctgggattg attctgatat agaagatatt
      301 agtgcctttt atgacatgct gatgaaaggg caaacaccaa tcaaagatgt gcctgaaaat
      361 cgctggaata ttgaagaata ctatagtgct gacagaaaaa aagcagataa aattattagc
      421 aaaaaaggcg gttttttaga taacacccat ttattcgatg ctgcttattt taaaatatct
      481 tctgccgaag cgaagcaaat agatccccaa caacgccttt ttctcgaagt ggctattcga
      541 gctttaaatg atgccaatat cccactagac tctttaaaag actctaacag cggagtttat
      601 tgcggcattt ccacacacga atacagccaa ctgaactata aagaccatat caaatttaat
      661 gcctacacac ccattggaat agcgaacagt gctgccgctg gcaggctctg ccattttttg
      721 aatttaaaag gacccagcat ggctgttgat acagcatgct cttcttcgtt cactgcactc
      781 tatctagcgg ccaccgcatt gagaaaccag caatgtgata tggcaattgt cggaggcgtc
      841 catttgagtc tctgtcctga aagttttatc ggattaacca aagccaatat gctgtcagcg
      901 acaggtcaat gcagcagctt tgacagtaaa gcagacggct ttgttcgtag cgaaggttgc
      961 ggagtagtta ttgttaaacg cttaactgac gccatacgag acaataacaa aatctatgca
     1021 ctcatcaaaa gcatggttat taatcaaaat ggcgatggaa caggccttgc agcccccagt
     1081 actaatgccc aaattgccat gcaccaagcc gcattggaac atgcccattt aactgcaggt
     1141 gaaattgatt acattgaaac ccacggaaca ggcacaacag taggtgatcc cgtcgaattt
     1201 aatgcgatcc aaagcattca ccaggggcat cactctcctc aaaaaccctt gatagtaggg
     1261 gcactgaaaa gtaatatagg ccatactatt tcatcatcag gcattgcttc tttaattaaa
     1321 gtactttgct ctttgcaaaa tgaagcgatt ccccccaatt tacattattc aaacccgaac
     1381 agcttgattg atcctgaaag tatccctgcg cttctacccg ttgaagcaat tccttttctc
     1441 aaactcaaaa ataaaaaaag atatgtccag gtatctaatt tcagctttac aggcacgaat
     1501 gtaagtgtca ttttggaaga agcgcctgag ttggaatcac acgagtcaaa gcaagacgac
     1561 aatgaaccaa aatgttttgt tgcctcagcc aatagtgaac tctcattgaa acaattgatg
     1621 tcacgctacg aacactattt gaaaaattct tcctcaacct tgggcgatat atgccatacc
     1681 ttgatcaatt gtagagacca ttacaaattt cgctgcgcca tcattgtaaa cgataaaaaa
     1741 gaactcatta aaaaaattga atcgaaagac tatgaaataa aaaaggtagt tctgcaaaac
     1801 aatcccagaa cggtattgaa tgatgccaaa gccatttacg aagcttactt atccggcatt
     1861 aatatcagta ctgatgataa aaatactgcg tacaacaaag tggatttacc gctctactgt
     1921 tttgacagaa aaccctattg gcatgaagtc agaaaacaag tcaagaatga tcattggctg
     1981 gattctttat atcaacaacc aatagaacaa caagtcgcaa tcattaaaac agagctcgca
     2041 tcccaaatca aagatctttt aaagaaagac aatatagatc cgcatcagga tttggaaact
     2101 ttaggtttaa ctacctcctt attggaatcc ctggatcaaa tcttccagga tatgttttcc
     2161 ccccgatata aggtgccttc tctccaatcc ttacgctact taaccctgga taaactggca
     2221 agacatttac aaaaaatgat catgccgcct gtcgtttacc gacaaccgtc aattaatgta
     2281 ctcaatgttg aacctattgc aattataggt atgagttgtc gtttccccaa agcagccaat
     2341 gtggatgaat tcctgtcttt gctggaaaaa ggagaaagcg gcatgatcga tatcccctta
     2401 gagcgctggg ataacgaaaa attctatgac tcggacgttg atgccttagg aaaactgtac
     2461 atcaagcaac tgggcttcat tgatcatgtc aaccattttg atgcggagtt tttcaatatc
     2521 agcccgcgtg aggctaagct catgtcacct caattgcgtg tttttatgga aaccagttat
     2581 cacgcacttg aagatgccaa tctttctctt gatgcggtca aagacactaa tacaggagtt
     2641 tttgtcggag tggggactaa tgaatatccc cgagttttag cttatcaagg cgttacctta
     2701 gaagatttga atatttactt tgcgacgggg aatgttttaa atgcgctggc cggccgtgta
     2761 gcctattcct ttgactttca tggcccaata caagcaatag ataccgcatg ctcatcctcc
     2821 atgacagcca tacacaatgc ctgcctgagt ctgcaatccg gtgactgtaa tatggctctt
     2881 gctggcggtg tgaacatcat ccttagccct gattctaaca ttactctttc aaaagccaga
     2941 atgctctcac ctgacagccg ttgcaaaacc ttcagtgaag acgctgatgg ctatggccgc
     3001 agcgaagggt gtggtgtggt ggttttaaaa cgtttaagtg acgccgttaa agacaacgat
     3061 aatattctgg cagtaatcaa aggcacctcc atcaacagcg acggtaaaag tggtggtttc
     3121 actgtaccta atggtgctgc gcaagaagaa gtcatcctaa gcgcattggc aaaagcgaac
     3181 ctgtcacctg gtgatattga ttttgtcgaa gcccatggga ctggcacgcc attagctgat
     3241 cctatcgaag tgaatacgct gaccaagatt tttagtgagc atcacaacaa agaaaacccg
     3301 ctttatatca gctcagtaaa aaccaatatt gggcactcgg aaagcgcatc cggtgtagcc
     3361 agtgttatca aaaccgtttt aagcctgaaa accaaaacaa tatttaagca tctgaacttc
     3421 aaaaagctta atcctgccat tcaattaatt aatacagtaa tccctcttga taaaaccgat
     3481 tggaccaaga aacaaggctt aaggtgtgcc ggggttagtt cttttggttt tagtggggct
     3541 aatgcccatg ccatcctaca ggaagctcct gaagacaaaa aagaagcacg cacccttccc
     3601 gaagaatctc tgctcgtttt atcagcaaaa agcaaaacct ctctggagtt attactggcc
     3661 agttaccaaa actatttatc caatacccgg gaagaatttg ccgatatctg ctacacagca
     3721 gcaacttgtc gcagtcattt tatgtatcgt gtagcaatta aagcgcaaaa tgcagcccag
     3781 gcggccaata tcataaaaag caatggatac actatttgcc atatcaccaa agaaaaagaa
     3841 tggactcaac aaccggacac acttgcagaa ttgcaaacag cctacctgga tggattcccc
     3901 atcaactggt ctgaatttta tcgctccctt ggccatcatt ttgcaaaagt aaaattaccc
     3961 ttatacgaat ttgaacgtat agaacattgg tttggtgaca aaaacaagtt gaaagatgca
     4021 cctctaccaa aggactggtg ttttcaaatc cagtggcaaa atgaaccatg tgataaaagc
     4081 aatcgaaagc tgagaggaaa tagatggctt ttaattggtc ccagacatct ggctgatggt
     4141 ttcaaagctc aaggcctgga aattgtctac gaagatgaag agtattctcg agacaaactg
     4201 gaaggcatca tttttgctga aagtttgcat ctggcaccac ctgacataga agctaatatt
     4261 gattttcaaa agaaaacctt aaaaaagctg ctgaacttaa ttaaagaact tgataaccag
     4321 tcaattgatt tgcaattgct cattcttacc agcaatggaa ttgctgaact ggcgaatgac
     4381 aaacaccctg tcaatataag caatagccct cttattggtt tctgcaagac tctcgcttta
     4441 gagctgccac aattcaaaac cattctcatc gatatggata aaatcgaaga ggacaaatac
     4501 cggacacaag tgatagaaga aatcaattat aaccatggaa gccgctatga acacatagta
     4561 gcttaccgtg gtggggaaag gctggcttcc cgtttaaaaa agactccgct ggaagatagg
     4621 cgacgactat tgcatggagt gaaaggacgc tatcttattg ctggcggctg cggtggtttg
     4681 ggattggtaa ctgcccaatc cttgctttcc tctggagcaa gagaattaat tctcacctca
     4741 agaaatatcg ataaaccaag gatcaaggaa gccatttata aaattaagtc ccattaccct
     4801 ggcgccaaca tccgggttgt cagtttagat attactgata gggaaagttt aaaaaattta
     4861 ttatcagagc ttaatgccga tggtcagtta aaaggcatca tccatgccgc tggtgctgcg
     4921 attaaagcgc ctttgcttga acatcaggat gaagacgttg attatttatt ttcagctaaa
     4981 gtaaaaggcg gatggtattt acatgaacta agtctggatt gcaacctgga tttctttgtt
     5041 gtctattctt cagtttcctc cgtatttgga agcaacaaag aatccgttta tagtggaacg
     5101 aacagtttcc tcgatgcctt gatcgcagag cgtcagcgta tggggttacc tggcaccgct
     5161 gttcagtggg gaccttgggg tgaagtagga atggcccaaa aacgctcgcg tgatgaagga
     5221 ctaaaacaag cgttaatcag taatgcacaa ggccatgtaa tcattaaatg tctcatcaac
     5281 gacgaattca aacacgccac tatcatatct ccagaatacc tcaaatttat gctggatttt
     5341 gtacccaagc ccctacctgg gttttatgga caacttgatg aggagcttga aaaaatacca
     5401 caacaacatc atcaaagccc ggcaaaaaat ctgtctccat ggcttaatca atatctggaa
     5461 attagtgaag aggagcgatt taaagcctgt aaagcaatgg tatctgatat ttgcacagga
     5521 atccttgaac taacagaaaa tgaagactta aacgaagacg aaggtttttt tgagttgggt
     5581 tttgactcgc tcatgattac tgaattggcc agtgaactaa agaaaaagct ggaaccattc
     5641 cttaaagtta ccgtgaccat tggttttaat tatccatcca tcagtaagct tgccaaatac
     5701 attgaatcag agcttgataa gaacttggtc aagatacaaa gtccaaaatc aactcccaag
     5761 ccgattgatg atggtatagc aattataggc atgagttgtt tattgcctaa tgcgccagac
     5821 atcgctgcct ttgaaaaact gttggaagag ggattaagcg gtatcaaaga cattccaaat
     5881 gaccgctggg ataataacca atattacgat cccgatatgg aagcgcctgg taagtcctac
     5941 gttactaaac tgggattaat cgaaaatatt aaatattttg acgctgcatt ttttggtatt
     6001 agtccccgag aagcaaaatt gatggaacct cagcaaagag tatttcttga gtgttgctac
     6061 aaagcattgg aaaatgccaa ctacccttcg gaatctttgc gtggcagctt aacaggcgta
     6121 tttgcaggcg taggtcccaa tgaatattat gcgcaactgg aaaaatcagg gttttcaaac
     6181 gaagaactca gcgcttactc catcactggt aatgtactca atctgattcc aggccgagta
     6241 gcctacactt ttgattttaa aggcccgtca atcagtgttg acacggcatg ctcttcgtcg
     6301 ctggtcgcaa tccattacgc ctgccaaagt ttaaaaaaca aggaaattga ttatgctctt
     6361 gcgggtgggg tgaatatact gcttatgccg gaatctaatg taactttgtg caaggcaaag
     6421 gcattatcac ctgatggcca atgcaaaacc tttgatgaaa gggctgatgg atacgtcaga
     6481 ggcgagggtt gcggcgtagt attgcttaaa agactctccg atgccttgcg ggataacgat
     6541 cacatcctcg ccgttattaa aggctcagcc gtaaataacg atggtaaaac agctggatta
     6601 accgtaccta atggtaaaag ccaggaagaa gtcatgaaaa aagcgcttgg ccagactgaa
     6661 ttgtcgagaa ccgacatcag ttatattgag gctcatggca caggaacccc gttaggggat
     6721 cccattgaag tggatgcaat caacaaagtc tacggtcatc aacgcaacaa agagaacccc
     6781 ttatacctgg gtactgttaa aaccaatatt gggcatcttg aaagtgcatc aggcgtagct
     6841 ggcgttataa aaaccgttat cagcctgcaa aggaaaacca tttataagaa tttgaatttt
     6901 aataaattaa atcccaacat caaactggat ggtactcaac ttgctttaga aaaaacaaat
     6961 tggaagacga atacaaaact caattacgca gcggtcaatg catttggttt tagcggcacg
     7021 aatgcgcatg ttatccttgc ggagtttcct aaagaagaaa aacccaaaac ctcaaaaccc
     7081 tctcaaaaac atttactggt tatttcagcc cactccaaaa cggctttaga taatttaact
     7141 cagcgatacc aacagtattt agagacgact gaacatcatt ttggtgacat atgctttacc
     7201 gcagcgactt gtcgagatca ttatcattac cgtttggctt taaccgctga gaatgcgatt
     7261 gaagcgagtc gtatgctggg tgcagggcaa tttgccttgt cttatcatga aaatattaca
     7321 ctggacttac aaaacgacag agcgcttaat tccctgttaa tggattatct gcaaggaaaa
     7381 ccagttgatt ggaaatccta ctatcaatcc cttaaaggag agtttacaaa agttctgctg
     7441 cctaattata cctttgaccg cagcgagtat tggctggata aaaaaaacaa cgatagcatg
     7501 gccactcata aaggtgagca acaccctctt ttagggcaaa tgcttgccat gccaggaaat
     7561 gaatatctgt ttcatcagaa actggatttg gagaatttgg cttatatcaa acaacacagt
     7621 gtttttgaaa aagtgatttt tccagcaaca gcctatattg aatcgggttt ggcagcagca
     7681 aaatccatat ttcaatccag cgcttttcaa attgaaaaat tcaatatcga acgtcctcta
     7741 catcctaaac aaggtcagga gtttcaatta caagtcaaac ctaaaatgga tggacaatac
     7801 aaaatcaatc ttttcgcaaa acaagagaac gactggcaaa ctttctgtga aatggaaatt
     7861 catgccaagc cgacagcagc ccgggaatct atccatattg acgtgcttaa atcatccttt
     7921 ggcgataaag tagagctgtc ggacatatat gagcaatttc agaaaagctc attattttac
     7981 ggtgatgaat ttcgtgtcct gcaagagggc tatgttcgga cgaacagtgt tttggctaaa
     8041 gtcacattga caaaggcaag ccagggccag gactattatt atcatcctgt tctgcttgat
     8101 ggtgccatgc aaagtatctt gttattaagt gttaaaaatg acgagaataa aaattttaac
     8161 gccgagaatg ttacctacat accctatgcc ttcatccgca tgactgtatt tcaggaagcg
     8221 ccacgaagcc tatgggtgca tttaactaaa caaaatactg aagatgcgag cgatctttgc
     8281 gttaacatga atttatatga gcaatctggt ttattaattg ctcaaattgc aggccttaag
     8341 ttgcgaagag taacacgaag tcattttgtt tcttatgacg ctgctttaaa ccacctttat
     8401 tacacagaat ggagccccgt cccattaaaa accttacccc aacaaaaaat ccctgacttc
     8461 ttggccatta caaaaaatcc tggcaaagct aaagaaatat tggatggcct taattatcac
     8521 ctggttgaaa ggatcgatga ctgcacaaat atcgagaaca gcaacatagt attcctttat
     8581 gaacaaggac agtttcatga tctttttcat tgctgccaga tcttatttcg attgcgtccc
     8641 aagcgcttta tactggttac ggaacatgct tatgccattc aagaccatga tcaggtcaat
     8701 ccttatcaca caatggcctg ttctttttgg aaaagcttta gaaatgaata tgagcccaat
     8761 aaaaattata ccattgattt agactccaat agtcgcttgg ctacggcgtt aatgtattta
     8821 tttaatacag acacccatga cactcaaata gcagtcagag aatctcttta cttacccagg
     8881 ctaaagaaaa agcaattggt cagtaactct gcctcggaga gattaattga cggcaatgcc
     8941 acttatctta ttacaggcgg tactggagga ttagccaaac tcttgatgga atacctcatg
     9001 aggcgcggtg ccaggcacat tgccattacc agccgcgccc actatccgga tgacattaag
     9061 gcattaatag ataaggccaa acaaaatcag attgatatca aacattacat ggttgatgcc
     9121 ggaaattatc agaaaatgga gcaagtgatt ggagaaatac aacagagccc caacccattg
     9181 aaaggcgtat ttcatttggc aggtttgata caagatgggc tcatcgtcaa tttgaatgat
     9241 gaagcgatgc aaaaagtaat cagtgcaaag atggatggcg ctttaatttt acatcaatta
     9301 acccaaaata tcccattaga atggttcgtc atgttttctt catccgcttc cttgttaggt
     9361 gccaggggtc aggcaaacta tgccgcagct aatgggtttt tagatggttt agcccattta
     9421 aggcgccaac agggcttacc agcaatttct atcaactggg gacctttcca taccacagga
     9481 atggcagcca atttgactca tactctacag cattatggtt ttttaccatt ggataaggac
     9541 aacattgata ttcttgatgt gctcttgcct agtcaattgc ctcaaatttc accttgccct
     9601 atgaattggg atgtttactg caaacataca ccgaaacaaa cggaattatc cgcgctggtt
     9661 aaggcgaaac cactacctga tcagtctttt ttgaatgcac ttcgacaaca ttccaaagaa
     9721 gaaagcatct caatattgtc acaagcatta cgagaaattg cagcggatgt cctggctttg
     9781 gatgacagcg aacacatttc taaccaccaa gatttgtttt caacgggtct ggattcactg
     9841 atgtcaatgg atatcagaaa tcgcatttat gacaaattac aatgccaaac actaagctta
     9901 ccgattgaat attttattaa tactcctacc attaataaaa tcgccagaca tatttctgat
     9961 gaattagacc atgtttttga tagtcactct gactaccacc ctactgaaaa tccgtcagaa
    10021 agggaaattg cattatgtga ttttcaatat gtattctggg tgcttaacaa actggattac
    10081 cattttaata tcggtacaca aatacaatta cacggccgaa tcaataaaga ttatgtccaa
    10141 cgggcttttg aatttgttgt gaatcaaaac agtgtcttct ggatccattt taataaggat
    10201 aaaccaaccc aaaaattgca taaagttgga caatttgaat taatttacca ggatatttct
    10261 ctgaatcatg aaactaaggc gctgaatcac gaattccaaa acaatatatt gcgcgctatt
    10321 ccactcacca aacaaccatt gattagagtg tatttgtata agattaataa tgaattgcat
    10381 gaattacaca tcgtaatacc ccatattatt gttgatgacg catcatgcga aatggttttt
    10441 tgtcaattta aaaacaatta tgaaaaacta actcttggga aagaattagt atccaaccca
    10501 gaaaaaggct cttattttaa ttatgtgaaa caaaataata atcactatca aaaaaacctg
    10561 aaaaataaaa ttgatttttg gcaaaactat aataaaggct ttaaaatgct gagtttcggc
    10621 aatagatatc atttatccga tacctcaaaa caaacaaagc acttatttca ttaccctatt
    10681 gatcctcaac acgccgaaca atttataaat tggcataaaa cccaaaatat caatgtcagt
    10741 actggattga ttgccgcatg ccaaattgtt ttctacaaac taagccgcca aaataaaata
    10801 ccgatcattt taattcacag tggtagagaa ggtggggaat ataaatcaac cctgggtttg
    10861 ttctcggaat ataaaaggat aaatcttacc ttaaatgaga atagccaatt tattgattgt
    10921 attcattcaa ttgaagagca actcttaaaa acagccccct atcaaaaatg ttctcatctc
    10981 atcaaagaca gtggcttgaa aggttcgagc ttatcctttg gtcaatattt gactttggcg
    11041 ttaaataaga tgtttatgct gaaacacttt aaagaaagta agctccattc cattatcatt
    11101 gactattacc tgaaatattt aagtcgcctg ctatccttca aaaaaaacat ttcaatgaaa
    11161 caacgactaa atcaactatt taaattggat atgtcattgc aaaaaccgga aagattaaga
    11221 gttctcgtca gcgtaacccc cagctttttt accaaagaac gccctgatat gagttttgct
    11281 aatcttgatt acagctatcc taatcatttt ggatgtatgg ataggccgat aggaaatcga
    11341 acgctttgga tttatttctc caaaaatcag gatggtgaat atcaaatttc cattaatgcg
    11401 ccattaacaa tagaatgtaa aaataaaatc ggaagtggtc ttagccagtt tattacaact
    11461 tttgtagaga ataataattg taatattgct gaattaattc aagcaatgga ggattcctcc
    11521 attgcttcct gattttactg agcctggtta accgtagtat tattattttt tatttgttta
    11581 gggtttgatg atgaaggctg agttgaaact ttgggtacca caatatccgt tccaaatttg
    11641 ccttttatct tgacctcacc tggctgcaat acagcagatt ggccattggc tctacgacaa
    11701 cgctgggtat gacatttcat cttacaaact ccctggacac atccatagca tccccagtta
    11761 tcccaacagc aagcccaatt gccttcgatt gttgaggtaa aagttccccc tgccaactgg
    11821 cagacatctt tcatttcagc attcgatgcc cccgtattcg gcgcagcata aacatgaatt
    11881 gatacaaaac taaaaagaaa gaggaaaagc acccctgcca tccttaaaga tttcatgatt
    11941 cacctccttg gaatcgtact atcagcacct gcgaaaaaac ccaatttctt tgttaaaaat
    12001 tgctttcaat ttgttcattt agcttatcta aactccctca ttaaaaacat tttttgcctt
    12061 gaccttgggg cttttcgtaa gccctaacta tttaattatg gcaaaacctc ataattgttc
    12121 aagttttaaa caaaaagagg tcttgaaaca ctgaaaagca tccactcagg ttacttcatt
    12181 ttttctatgc caaatactga atagaataca aaaaaccaaa gttgccataa caatacccag
    12241 agcaatgaaa atcggtatct taaaccaagg tgcgataagc atcttaaagc ccacgaatac
    12301 taaaataaaa gcaagcccat atttgagaaa ataaaatcgt ttgtgcatat tcacaagcag
    12361 aaaatataaa gcgcgtaatc ctaaaattgc aaatatatta gaagtgaata caataaaagg
    12421 atcattggtt atggcaaaaa tcgctggaat gctatccaca gcgaaaatca gatcactgat
    12481 ttcaataaga accaacacca taaaaagagg cgttacgtag acaagttgat ttttcctgag
    12541 aaaaaatcgc tcaccatgta actcctgggt aattcgcaaa tggcttcgca tccaatgtag
    12601 tatggggttt tttgcgagct ctggtttctt atcagcaaaa atgaacatct tgattcccgt
    12661 tatcatcaga aaaaatccaa aaatataaag aatccaatga aattgattca ctatccatat
    12721 ccctaataaa ataagaatca gacgcataac gatggcacct atcacaccat agattagaac
    12781 acgatgttga tatttagccg gtattgcaaa atactcaaag agaataagaa aaacaaaaat
    12841 attatcaaac gataaagact tctctatcag ataacccgta aaaaattcaa gggctttttc
    12901 attggccact gaaaaagtaa aattctcaac aagataccac catagaagaa agttgaaaat
    12961 aatagccaga gtgaaccaca atatggtcca ggataaagct tcttttgtgg aaacgcgatg
    13021 agcttttcct cccccaaaca caaacaaatc aacaaacagc ataatgagca caaatgctaa
    13081 aaaacctaac cacatccacc attcactgat tgaagtcatt aatttctacc atgtcttttc
    13141 tatggttttt ataccacatt gatatgtttt cattatagtc atagtattag aaattatgcg
    13201 cttcaaaaaa tatttatatt catttgaaaa aacctaggaa ggaggaggag aagaaggatc
    13261 caaagtagcg atcaattgaa taaccccaaa ctcaccattg gttttcctgc ctacagttaa
    13321 ttgaatattc agtaattgag aaactcgcat ggtatcgttt ttataagtga cttttaaggg
    13381 caatgctata atccaatggt tctctctggt ttcaatgaac tctgctaccc cattaacttc
    13441 actagtcata gtgagtcttt gcattttaat tgcctcgata ttccctgatt tcatcatggc
    13501 agaattaaac tcaatccatc cgttttctgt ataacatgat tgtaatttat ttagctgcga
    13561 ctcaatagaa gtaaaatcaa aagcaaaaga ttgtgttgcg gcgtgttcag cccattgtaa
    13621 aataatattt ttatctgttt ttatagtttc ttcagggatt ttatagtgac aagaaaccgt
    13681 ttcttttggc tttgattgct ccggttgttt gagttgaatg ttttgagagc cgaggaacaa
    13741 ttgaggtgat agctgaagag aagatgaggc cagtatcgat tttaaagatt gtaacaccct
    13801 gttagagtca aggctcttat tctttaccgt caagtgattt atcatctttt gatctaaagc
    13861 taaattaaca tcaaggcgtt gagtgattct ctcatcgtct ttttgataaa caatttttat
    13921 aggtaattta atatcccatt gattttcttt aacctctata aactggggct ctccttctat
    13981 ctgagcactt aaaatcggct tttgcttatt gattgcctca atattatctg atttatctct
    14041 tatggattgt tgatattgag aaaaagcacg ccataagggc aagggattaa ttcgagtaat
    14101 caatgaatta agttgtaatt tagaagaatg caaaccgatc tggcttaacc agtttggata
    14161 gagttttcga cttttgttgc caaaatcact cagagtgctt ttgttagaat cagaatcgtt
    14221 aacaagggat ttctgttggg ataaagcaag atatggagtt tttgcagatt caggcatatc
    14281 aaatgattgc attctcacaa gattatgagt gggatttaag tttggcattg gttgaataat
    14341 tgccgctctt tgcgaagccc tgttactcaa agcaagattt cctttagaac attctccatg
    14401 atttgaaaat aaaaacacaa aaaaagaatc tgttattttt cttgcactat ctaaactggc
    14461 cttttttctc tcagccagta ttatgcccat acttttaatt acctcatcag ctggcgtcac
    14521 cttctgagct agtggcgcca gacgaggaat agcaatcatt tgcataatcc ccaaactatc
    14581 tttatttttc cagcctatgg tcagataggc attaagaaaa tgtgtcactc tttttttatc
    14641 attctcataa acaactttta agggcaatat aattttccat cgattatcca aagcctctat
    14701 tatttgagcc tccccatctt tatagccctt tacttttaat ttttctgctt taatggtttc
    14761 tatattattt gatttatgta aagcattttg catttgcccc cacccctttt cagtatagca
    14821 agagtgcaat tgatttaatt ggtcctcaat agacgaaaaa ttgaaattaa aagacagaat
    14881 agccgcatat tcagcccaat ttaaaatgat attcgaatca atatgagaaa tttcaggtga
    14941 gattttataa tcacaatcaa taacattgtg atagacaata cccttttttt caatggattc
    15001 ggttcgcttt gcaatatctc tgctaacagc atcctgagag taggctttca tactcaataa
    15061 aataagaaat acaccataga agagatgttt tggcataaaa agattcctta ttctgtatct
    15121 acaaatccct tacgaacgtg aaaatttatc ggataattta atttattcta ctgtttaata
    15181 gtttggaggt aactagttaa tttttcaaat attttactat tctaatttcg acctatggaa
    15241 agaattgcta ttcagcacat gttttctttt tagactataa aaatctgtca tttttctact
    15301 tgaaatttta ttaattgacc ctatttaaac atacaactac atagggtttt acacttcaat
    15361 tgtgagacgt ggagcagcca gtagggtgct cggttaatat taatcttggc ctcaaagtta
    15421 aggagatata ttatgaacac tacctcatta tcactaaccc cgttactacg gcattccgtt
    15481 ggttttgaac gattcaatga tttgtttgaa tcgatgcgaa atgccgatga ttcaacctat
    15541 gcctatccgg cctatgacat tgagaaacat ggagaagaca gttatacgat cacaatggct
    15601 gtcccaggat ttcaggagag tgatctgaac atcatggtac aaaatgatca gttaacggtt
    15661 agtggccgca ttcaagaaaa agaaaccaag gagtctgaat acttacaccg cagcattgtg
    15721 acgcgtgctt ttgaacaaac ctttcgactt gcagatcaca tgcaagtaac aggcgctgaa
    15781 ataaaaaatg ggctattgtc tattggcttg attcgtgaaa taccggaaga agcgaaacca
    15841 cgcatcattc caatcaagag tatttctgat caagaaagca ataaaaaagg caaaaccctc
    15901 gaaccggaaa gcaagaaaaa aatttccaat taaaataatg tgccggggga tatggaaaat
    15961 ccggcacgga ttttagatag aatagccaga tttcgcctgg caatgattaa tttgatgtta
    16021 accagataag tctaataata gcaataactc aattgactta acccagattt aatggaggtt
    16081 acgactatgg acattaaaaa acttgctccc tggaattggt ttaaagagga agaagctcaa
    16141 aataagagcg taccgcttag taccactgga caaagctgca tttctcctct gagccaactg
    16201 catcaagaaa tagatagaat atttgataat gtcttaagaa gcgttgggat ttctaatctg
    16261 gatatagaaa aaacaccctc taactcagcc caaaatctgt tgcttaaacc ctgtatcgat
    16321 attgcagcaa ctgataagga atataccatc acagtagaag tgcctggcgt ggaagaagat
    16381 catattaagt tggaattaag cgataacaca ttgatcatca agggcgaaaa aaaacatgag
    16441 tcagaaaaaa aagacaaaga catctataga atcgaacgcg cttatggctc atttcaacgc
    16501 gtgttatcac ttccagaaga cgctaatcaa gaagacatca aagctcagat aaaaaatggg
    16561 gttctcacca ttacaatgcc acgtaaggag gtatccaaac ccaaaggtaa attaatagac
    16621 attaaaaaag caagctgatt tattgccaga aatctatgag caatgtgccc atataagtat
    16681 cctctttaat tcaatagaaa gagggggcca agggaaaagt tagagttgga taaaaatcta
    16741 ctctga
//
LOCUS       DACTJE010000060        16343 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.060, whole genome shotgun sequence.
ACCESSION   DACTJE010000060 DACTJE010000000
VERSION     DACTJE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16343
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.060"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            69..1040
                     /locus_tag="I9760_001509"
     CDS             69..1040
                     /locus_tag="I9760_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043281.1"
                     /translation="MLKKTSLALLGLAASGYALAGTMGPVCTPGNVTVPCEAKRWDLG
                     VQALYLKPVYDADMAYRQVHVAPTRYDDDQGWDWGYRLEGSYHFNTGNDVTLTWIHYS
                     DFANPSGLVGRIDIPPLPPALAFPRLFSAIYKNRFEQVNLVMGQHADFGLVKDMRFYG
                     GLQYANIEIDNTNYYFGAFPQLGGVNLFDNTDYKGVGPVVGIDYAYALTNALSVTANG
                     AASVLYGKGKYNIGYVALPTGSSGAVVRSIYANKRTIVPSLEAKLGLNYAYNMAQGVL
                     NLEGGYQVMNYFNALHTQFLQNMANPVTNSDYGLYGPYIGAKYIGNA"
     gene            1337..2929
                     /locus_tag="I9760_001510"
     CDS             1337..2929
                     /locus_tag="I9760_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbtU family siderophore porin"
                     /protein_id="HAT5043282.1"
                     /translation="MKFKKIILALACLSSPLYADQDQQLKSEIQRLQHQAEDLQAQLN
                     RLQKQLANHKSSQQKHEQQAAAKPAEPQSKPTVKSGAAIEEKYHSSKVEVHAPDAHPE
                     SISFYPTALIADNRVVTYIAGTPVVSSPFLGDRPAFDGSDYIVNISSINRDVRLMQQR
                     RRLYRAYQKIGYPIPNMPIISLSGKAEPAATFNNPFRSTNTDGDITLGSSELDVAAAL
                     NENVEAYIAIAYDESPPAIGPRVNNSAFNLNMGFVNIGNLDKSPLYFTAGQVYVPFGR
                     YSSAMVSSPVTMNLARTKTRPVIFGYKSQADTGPFGAVYGYRSDTTLGRSGVGGVNLG
                     YIFGFDNDINGEVGGGFISSIADAGGMQSTGANVGTTFGGFGSITNGNENVRKTKAAD
                     VHGHVGYDRYNLTLEWVGAIQSFRPQDLSFNGQGARPQAAQAELGMTFMAFNRPASIG
                     VGYQWTKEALALNLPKRRYVGVFNISIWKDTVESIEYRHDIDYGLTQFANGAAPQGFV
                     NLPTLGTGKSADTVSAQIGVYF"
     gene            3136..4641
                     /locus_tag="I9760_001511"
     CDS             3136..4641
                     /locus_tag="I9760_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HAT5043283.1"
                     /translation="MHRDCVRQEGLHKLNNFFCKLAVVKRILQGMIFKRRLSDSVHED
                     SDSVATKQSPASVTCEKGLSKLMQTSCIFIFLTLFLLIGLQTSFAAKIVELNIKGPIG
                     PATVDYMERGIKSAQDADLIVILIDTPGGLYDSTRNIIQLFLLSDVPIVTYVSPTGAR
                     AASAGTYLMYASTIAAMAPGTQMGAASPVSLGTGFSEGEKDEKKKSTMENKVTHDAVA
                     TIRSLAQLRGRDPDFAEKAVTEGKSITANEALSKGVVNYIAKNRDDLLSQIHGIKVSQ
                     NNKTITINTESPDIQVINPDWRTRFLSVITNPTVAYLLLLLGIYGIFFELVNPGYVLP
                     GVVGAVSMLFALYALQLLPINYAGLGLIILGILFVIAEAFTPSFGALGVGGTVSFILG
                     SIMLMNTEHLAFQIAWSAIWAMAVLNILIFVLVLGMLIKSRNQKIRHGLETLVGAKGR
                     ALGDINLEGQAVIKGEIWNVHSSSPIAANKSVKVTRASGLLLEVEEDQSVY"
     gene            4684..5439
                     /locus_tag="I9760_001512"
     CDS             4684..5439
                     /locus_tag="I9760_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HAT5043284.1"
                     /translation="MGPFLVILLVAIGLLLASMFKVFREYERGVVFMLGRFWRVKGPG
                     LIIIIPIIQQVVRVDLRTIVMDVPSQDVISRDNVSVRVNAVVYFRVVVPENAIIQVEN
                     YFEATSQLAQTTLRSVLGQHDLDDMLAEREQLNSDVQKILDAQTESWGIKVSNVEIKK
                     VDLDESMIRAIAKQAEAERDRRAKVIHAEGELQASEKLLQASQVLAQQPQAMQLRYLQ
                     TLATIAVNNNSTIIFPMPMEIGDILTSMAGKKK"
     gene            5678..6244
                     /locus_tag="I9760_001513"
     CDS             5678..6244
                     /locus_tag="I9760_001513"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAT5043285.1"
                     /translation="MSIKSDRWIEKMALEHGMISPFQAGQVRENQNGRIISYGVSSYG
                     YDVRCSNEFKIFTNINSAIVDPKAFDENSFVDVQSDVCIIPPNSFALARTVEYFRIPR
                     NILTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTTLPAKIYAYEGVAQMLFL
                     EANEVCAVSYRDRNGKYQGQTGVTLPRT"
     gene            complement(6260..6571)
                     /locus_tag="I9760_001514"
     CDS             complement(6260..6571)
                     /locus_tag="I9760_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAT5043286.1"
                     /translation="MIKSELIEHIAARMTHLTEKQVADGINRILELMSEALIHGQRIE
                     IRGFGSFSLHYRPPRNAHNPKTGEKVVTEAKYSPHFKPGKELRERVNSSRAKFPLLDK
                     D"
     gene            complement(6824..7540)
                     /locus_tag="I9760_001515"
     CDS             complement(6824..7540)
                     /locus_tag="I9760_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HAT5043287.1"
                     /translation="MLDFISSKTLFLCLILFLILFWLCFDKARKELRINRWQKKLNLK
                     HHAQIFHLLYGDVNGFMISQQARKNRDAIEYVYGEIEFLPFTALLSMVNIDHNTVFYD
                     LGSGTGKAVVACAMVYPVHKSVGIELFPNLHQCACERLQKLAAMEGYTESSKKVSFIL
                     GDFLTVDLSEATLIFINSSTLFGATWEALNTRLNSLPQLSTVITTSKTLSSSRFKLVT
                     RAKIQMSWGVVFAFIHKKEN"
     gene            complement(7545..8270)
                     /locus_tag="I9760_001516"
     CDS             complement(7545..8270)
                     /locus_tag="I9760_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043288.1"
                     /translation="MEHYQEDDLARIKELLRHTGEFIAYFEFVEAKMMEWREEIEQQA
                     GRVLHIAQSLQNELTSMQNLISQSGVTHFKISAEKALAQGETHLHSLERTCNQFTQIF
                     QQQQERLNALTENCIDRIERHTGQATQTLTMQLSKYDVQHFHRIANESCDHVDRVAND
                     AINKSNKMLNMLQFKVGLFAMLITVLTAFVIVIYLNDEMPWEMHHQAMSERQAGKVLL
                     QAWPKLSQKEKAKILNEDGLKPG"
     gene            8855..9898
                     /locus_tag="I9760_001517"
     CDS             8855..9898
                     /locus_tag="I9760_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043289.1"
                     /translation="MPRARFFKAETNSSQVQQSFINVGGEQFHAAAVALIDYFKRSPR
                     INDDTLKKILERFYFHFPKYIPSQPYLTPSERMSMLINGARKSEIVECFAYVLRQLAI
                     DEIYAKPLNYRDIFAEFDRDTPKSLLRQPSTPLPASALRALSHVIGTNITLSVVENGK
                     ELRKREVYVGDNLNSAKPGITIQVQQNNRYFPAVKNKGDFVYVGQLAVSPPKPVESSN
                     TSGKDTIADMVDLIASDNKKLLQTYKQWHQNFLTMLKLGEITTQRMINLYIKFLPSEK
                     GLVVDSAEFFSRLSEDSDKPVIPDYSGENKDGVNQLLASALAGWISARQVDVDQLFDH
                     LENRPSSVSMSVG"
     gene            complement(9993..10943)
                     /locus_tag="I9760_001518"
     CDS             complement(9993..10943)
                     /locus_tag="I9760_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HAT5043290.1"
                     /translation="MIYPNILATIGHTPVVKINRLGKDLECELYAKCEFFNPGGSVKD
                     RIGYEMVVKAEKEGRIKPGDTLIEPTSGNTGIGIALAGAVLGYKVIITMPEKMSQEKQ
                     SVLERLGAIIYRTPTEAAYNDPDSHISLAKKLQAEIPNSHILDQYANPNNPNAHYFGT
                     AQEIIDDFGKDLHMVVAGVGTGGTITGIAKRLKEFNPAIKIIGADPEGSILGGGTEIK
                     SYHVEGIGYDFFPDVLDNTLIDAYIKTNDADSFRTARRLIKEEGLLIGGSCGAAMWAA
                     LQAAKSLSKGQKCLVILPDSIRNYMSKFANDEWMKEMGFL"
     gene            11522..11788
                     /locus_tag="I9760_001519"
     CDS             11522..11788
                     /locus_tag="I9760_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043291.1"
                     /translation="MINYKTIKWRYGLIALFALGVSGCYFDGTSPQERGYSYQENSMP
                     SSGGQTTASNQSQPQQKSYASKDTSQKSTPGPKRTAAPQLPVIQ"
     gene            12029..12166
                     /locus_tag="I9760_001520"
     CDS             12029..12166
                     /locus_tag="I9760_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide"
                     /protein_id="HAT5043292.1"
                     /translation="MDKLNWEQPEVCAIPIDSVSASTEEADVQDGSSESGLSSSSSSG
                     G"
     gene            12254..13219
                     /locus_tag="I9760_001521"
     CDS             12254..13219
                     /locus_tag="I9760_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HAT5043293.1"
                     /translation="MEYGNLIDILNTNETALTSFSAPEILKTAQRMRLTNSVYTILRE
                     HYGIPASQLANFKLVLKENRDRAIRKLFLSIKINELFQQYNIPVIHLKGLPLSQKLFG
                     DPLIRQSCDIDLYISPQHLEQADEVFFENGIIRDKYSFLHDVNKLKKHRVLKDVSYVA
                     EKYGIKIELHWRLFRWKDKFNQPFEFYWNNKVPILVGGKNLFYTLSPIDEYLYVAVHG
                     ANHGFESLHWLYDFARYSTLLSQDDSRRIVELVNNLSIQTEYQMATYCAERIFPSVFT
                     ESGNAGLLPNSLTRKALISLIYHSKSTYRSKRRILRMMANKFYYI"
     gene            13361..15187
                     /locus_tag="I9760_001522"
     CDS             13361..15187
                     /locus_tag="I9760_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HAT5043294.1"
                     /translation="MNALYGVLRFDGQPFDRELLERAKFNSPVWRADHSELYYNDFYA
                     TASTQRFITPECALQTAPYHDKESGVIANGDLYLTNHSSLANWLGVSSKNSDLYLATR
                     AYLKWGEKCVNYLAGNFFLVFYDLRKKRLFIAIDHFNFCCCYYLFVPGKYFIFSNTFN
                     TFKVFHDSLAVNHGAFHKLAAKQKIGQETCLKSVKKLTAARYMIVSDQNLQEQQYWDL
                     RKTQQTGQCQSREDYYSAFRNEIQQSIRQSIRTDAEIMAHYSGGLDSSSIASIAALQL
                     AKENKTLMAYTAIPKYLSGDSHRKGCKYHELDRVQSACRYYDNIETIVYQSSEDLSIF
                     DKLQVFYPYLDQPFKAVFNMEWILAGLEMAAAKNCRILLNGEQGNGSISWKGVTLGNY
                     LNQRVKFTRHLLKYHMKLGKQFIKSGSLNLKKNRTDLNEMKKNFLEPHYSLLRGKNRL
                     ETRSYMRSIYLWFGVEVIDPTSNLDLVQFCYDLPEWVYHGGNKYFEIRRLPKLKRRLL
                     VRDALEGLVPPEIRHNPYRGEQAADWYYQFNKYGHAWGRLLADIAEKHPLIWEIYPKK
                     YLLDLFANHPISPQSRNFQDVNMHLVPALSTAFFINYLNDLY"
     gene            15213..15527
                     /locus_tag="I9760_001523"
     CDS             15213..15527
                     /locus_tag="I9760_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HAT5043295.1"
                     /translation="MSNHLQQHKYQFEYKTNTILERYDLVFFSSVDEDLILLDEEASH
                     YLVVNSVGYKIWNLLESKKTVSQLVSGLMEIYKVDFDTCFNDIKAFIQQMVQHKLIKV
                     CS"
     gene            15585..16055
                     /locus_tag="I9760_001524"
     CDS             15585..16055
                     /locus_tag="I9760_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HAT5043296.1"
                     /translation="MTEIKKIRYAHLFLVLLDKGKTRVHVAVLIVYYLIVSRIITWLY
                     PFNRYAKSFGHKHAETLYEDFDHLYWLKCCQKLFPKINRLLPWKSACLQQSVAAFLLL
                     RRKNMPTTIYFGMKREGDEVIGHVWLRCGKHYITGGNGVGYTIFCTYALDRVKN"
     gene            16066..>16343
                     /locus_tag="I9760_001525"
     CDS             16066..>16343
                     /locus_tag="I9760_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5043297.1"
                     /translation="MAEFYKKYAGKYKDFLDFIRFLYEYSPFQLVFSQAMVILSSFLG
                     GIGVVSLVPMLSYSGWLAQNKSGSDLNWLSSLWQQLPMHDGQIPITFV"
BASE COUNT         4910 a         2986 c         3421 g         5026 t
ORIGIN      
        1 ttaagtaatt aagttctttt tgagcccatc accaaaatga tgggcttttt ttttgaaatg
       61 gagtccatat gttgaaaaag acaagtttag ccctattggg attggctgca agtggttatg
      121 cattggcagg aactatggga cctgtttgta cgcctggtaa tgtcactgtt ccctgtgaag
      181 caaaacgttg ggatttaggt gtgcaggcac tctatctaaa accagtttat gatgccgata
      241 tggcttatag acaagttcac gtagccccta caagatatga tgatgatcag ggttgggatt
      301 ggggataccg attagagggt tcttatcact ttaatactgg taatgatgtg actcttacat
      361 ggattcacta cagtgatttt gcaaatccta gcggtcttgt cgggcgaatt gatatccctc
      421 cattgccgcc tgcattagcg ttcccacggt tatttagcgc aatatacaaa aacagatttg
      481 agcaagtgaa cctggtgatg gggcaacatg ctgattttgg cttggtgaag gatatgcgat
      541 tctatggtgg tttacagtac gccaatattg aaattgataa tacgaactat tattttggtg
      601 cgtttcctca gctaggcgga gtcaacctat tcgataatac tgattataaa ggggtaggac
      661 ctgttgtagg aatcgattat gcctatgccc taaccaatgc attgagtgtc actgcgaatg
      721 gagcggcttc agttctatac ggtaagggta aatacaacat cggctatgtt gcattgccta
      781 caggctcttc tggcgcagta gtgcgatcca tctatgcaaa taaaagaacg atagttccca
      841 gtctggaagc taagcttgga ttaaattatg cttacaatat ggcccaagga gtattaaatc
      901 ttgagggtgg ttatcaggta atgaactatt ttaatgccct gcatacacaa ttcctgcaaa
      961 acatggcaaa cccagtaacc aatagtgatt atggactcta tggaccatat attggcgcaa
     1021 aatatattgg taatgcctaa acgaagtcct ttacttaaat gcgttcgtca tcgacgaacg
     1081 cattgcttta aaatactgct taaaaatctt gaaaaaaatt ctttaacaaa aaatataatc
     1141 acggtaatcc attctaagtt taaccaacta aacccctaat ttattgatgt atagacaata
     1201 aactataaat gcatacaaat aaatcaattt attttaaatt ttagcggagt gatcctatag
     1261 ccagccagta attgccgcgc tataatccgg tggctatccg tttaggatgt aaaaataata
     1321 aacaggagta ccccgcatga agtttaaaaa aattattttg gccctcgctt gtttatcatc
     1381 gccattatat gcggatcaag atcaacaact gaaaagtgaa attcaacgat tacagcatca
     1441 agcagaagat ttacaagccc aactcaaccg attacaaaaa caattagcaa atcataaaag
     1501 ctctcagcaa aagcacgagc aacaggccgc cgcaaagcct gcagaaccac agagtaagcc
     1561 gacggttaaa tcaggggctg caatagaaga aaaatatcac tcatccaaag tcgaagtcca
     1621 tgcgcctgat gcccacccag agtcaataag cttttatcca acagcattga tagccgataa
     1681 ccgagtggtt acttatatag cagggactcc tgtagtaagc tcgccattcc taggagaccg
     1741 gcctgcgttt gatggctctg attatatagt aaacatttcc agtattaacc gagatgtacg
     1801 tttaatgcag cagcgacgcc gtttatacag agcctaccaa aaaataggct accctatccc
     1861 taatatgcca attatttcat taagtggtaa ggcagaacca gcagcgacat ttaataatcc
     1921 atttcgatca actaatacgg atggtgatat cacgctaggc tcaagtgagc tggacgttgc
     1981 tgctgcattg aatgaaaatg tcgaagctta tattgcaatt gcctatgatg aaagccctcc
     2041 cgcaattggc ccgcgggtta ataactcggc atttaatttg aatatgggat tcgtgaacat
     2101 tggtaatttg gataagagcc cgttgtattt tacagcagga caagtctatg ttccttttgg
     2161 ccgttattcc agtgcaatgg tcagttcccc tgtgacaatg aatttagcaa ggaccaaaac
     2221 aaggcctgtc atttttggct ataagtctca ggctgatacc ggtccttttg gcgctgtcta
     2281 tggctacaga agtgatacca cattgggtcg ctcaggagtc ggaggcgtta atttagggta
     2341 tattttcgga tttgataatg acatcaatgg tgaggtaggg ggcggtttca ttagctctat
     2401 tgcagatgcc ggcggtatgc aaagcacagg cgccaacgta ggaacaacgt ttggtggttt
     2461 tggctccata acaaacggta atgaaaacgt tcgaaaaaca aaagcagctg atgtccacgg
     2521 tcacgtaggg tatgatcgtt ataatctgac tttggaatgg gttggtgcca tacaaagttt
     2581 tagaccccag gatttaagtt ttaatggcca aggtgccaga ccccaagctg cccaagcaga
     2641 actcggtatg acttttatgg cttttaacag accagcctcc ataggagttg gttatcagtg
     2701 gacaaaagaa gcattagcac tgaatctacc taaacgacgc tatgtcggag tgtttaacat
     2761 ttccatttgg aaagacacag tcgagagtat tgagtacagg catgatattg attatggact
     2821 tactcagttc gctaatggtg ctgcacctca aggttttgtt aatcttccga ctttgggtac
     2881 aggaaaatct gccgatactg tttcagccca aattggtgtt tatttctaaa ttatttccat
     2941 tcaaatagca ggcttgtaac aattcaatcg gttacaagct gatgtgttta gggggctgct
     3001 tacaccggat gcggggtaag tatcctgccc agttcaactc actttgataa tactgaattg
     3061 acgtgaccag cccctttgtt tttttcaacc tgtgctatct tttattttat aactacctct
     3121 taaagggatg taaaaatgca tcgggattgc gtcaggcaag agggactgca caaactcaat
     3181 aattttttct gcaaattggc tgtggtaaaa agaattttgc aaggaatgat attcaaaaga
     3241 agactatctg attcagttca tgaggattca gattctgttg caacaaaaca atccccagcc
     3301 tcagtaacgt gtgaaaaggg actgagtaaa ttaatgcaaa caagctgtat ttttatattc
     3361 cttaccttgt tccttttgat tggattgcaa acttcatttg cagcaaaaat tgtagaattg
     3421 aatataaaag gaccaattgg gcctgctaca gtagattata tggaaagagg tattaaaagc
     3481 gctcaagatg ctgatttgat agtgatttta atcgatacac caggtggttt atatgattca
     3541 acacgaaata tcattcagtt atttttactt tctgatgtgc ccatagtgac ttatgtaagt
     3601 cctactggag caagggctgc gagtgcagga acttatttaa tgtatgcgag tacaatagcg
     3661 gctatggcac ctggcactca aatgggggct gcaagtcctg tcagccttgg cacaggattc
     3721 agtgaaggtg aaaaagatga gaaaaaaaaa tcaaccatgg aaaataaggt aactcatgat
     3781 gctgtcgcta caataaggtc acttgctcaa ctaagaggac gcgatcctga ttttgctgaa
     3841 aaggcagtga cagaaggtaa atccataaca gcgaatgaag ccctcagcaa gggagtggtc
     3901 aattatattg caaaaaatag agatgattta ttgtctcaaa tccatggaat aaaagtcagt
     3961 caaaataaca agacaataac tattaatacc gaaagccctg acattcaagt aatcaatcca
     4021 gattggagaa cccgctttct atcagtcatt accaacccta cagtggctta tttattattg
     4081 ctacttggaa tttatggaat tttctttgaa ttggtgaatc caggttatgt tttacctgga
     4141 gtggttggcg cagtttcaat gctttttgcg ctgtatgcgc tgcagctgtt gcctataaac
     4201 tacgcgggtt tgggcttgat tattttaggt attttatttg tcatagccga ggcatttacc
     4261 ccaagttttg gagcgttagg tgtaggagga actgtttctt ttattttggg ttccattatg
     4321 ctgatgaaca cggagcacct ggcttttcag atagcgtggt cagcaatttg ggctatggca
     4381 gttctgaata tattgatttt tgttttggtt ctggggatgt taattaaatc tcgaaaccaa
     4441 aaaataagac atggcctgga aacgttggtg ggtgcaaaag gcagagcgtt aggagacatt
     4501 aatctggaag gccaggctgt aattaaggga gagatttgga atgtccattc aagctcgccc
     4561 attgcggcaa ataaaagtgt taaagtcacg cgagcatcag gattattgtt ggaagtggag
     4621 gaagatcagt cggtttatta gttggttatt ccatgttgaa tgaaagggat taaggagaat
     4681 gagatgggac catttctggt tatattactg gtagcgatag gcttgctact ggcttcaatg
     4741 ttcaaggtat ttcgggaata tgagcgtggg gtggttttta tgctgggtcg gttttggcgc
     4801 gttaaaggtc cgggattgat aatcattatc ccaattattc aacaagtggt acgtgttgat
     4861 ttgcgaacca tagtcatgga tgtacccagt caagatgtta tctcaagaga taacgtttct
     4921 gtacgcgtca atgctgtggt gtattttcgc gttgttgttc ctgaaaatgc aatcatccag
     4981 gtagaaaatt attttgaagc aaccagccaa ttggcgcaaa ccacattgcg atcagtatta
     5041 ggtcaacatg atttggatga tatgttggct gaacgtgagc aactgaatag tgatgtgcaa
     5101 aaaattcttg atgcccaaac agaaagctgg ggaatcaaag tttcaaatgt tgaaatcaag
     5161 aaggtggatt tggatgaaag tatgataagg gcaatagcca aacaggctga agcggaaaga
     5221 gacaggagag cgaaagtcat ccatgcggag ggagagctgc aagcttcgga aaagttatta
     5281 caagcctctc aagtactagc ccagcaaccc caggcaatgc agctaagata tttgcaaact
     5341 ttagccacta tagcggttaa taacaattca acgataattt tccctatgcc aatggagata
     5401 ggtgatatat taacttccat ggccggcaag aaaaaataga ccttgaacag tggcataagc
     5461 cccaaatata cctaagtgta tagagtgctg ccattggata tgagaatatg gtgatgagtt
     5521 tttttattcc acaacaggtt gtggtatcag tattttttta aaatgagggc ttttatcgaa
     5581 gtttcaatca tcttattctg tataatttcc caaaacaaac tttcgtttca ttgactttta
     5641 tgatagaaat ctatcttttt aagaaaagag gaatatcgtg tcaatcaaat cagacagatg
     5701 gatagagaaa atggcccttg agcatgggat gatttctcca tttcaggccg gtcaagtgcg
     5761 agaaaaccaa aatggtagga ttatttctta tggcgtctcc agttatgggt atgatgttcg
     5821 ctgttcgaat gaattcaaga tttttacgaa tattaattca gcgattgttg atcccaaggc
     5881 gtttgatgaa aatagctttg tagatgttca gtctgatgtc tgcattattc cccccaactc
     5941 gtttgccttg gcaagaactg tcgaatattt tcgtataccc cgcaatatat taacgatatg
     6001 cttaggtaaa tccacttacg ctcgttgcgg gattatagtg aatgttactc ctctggaacc
     6061 ggaatgggag gggcatgtta ctctcgagtt ctccaatacg accactttgc cagccaagat
     6121 ttatgcgtat gagggagttg ctcaaatgct gtttttagag gctaatgagg tttgtgctgt
     6181 ttcttatcgt gatcgaaacg gaaagtacca gggacaaacc ggggttacac taccacgcac
     6241 ttaggatgaa aaataaaatt taatctttat ccaaaagtgg gaacttggct cgcgacgaat
     6301 tgactcgctc tcgtaattcc tttccaggtt taaagtgagg actgtatttt gcctctgtta
     6361 ctactttttc acccgtttta ggattatgag catttcgcgg tggtcggtaa tgaagagaaa
     6421 aactgccaaa gccacgaatt tctattcttt ggccatggat aagtgcttca ctcattaatt
     6481 caagtattct gttgatgcca tcggctactt gcttttcagt aagatgcgtc attcgagcag
     6541 cgatgtgttc aatgagttcg gatttaatca tatactcacc tcgttttgtc gcgtatttaa
     6601 tagaccgatt gcatcctcta ttattttagc ttggttgcct atgtttctat tcgctttgta
     6661 tcttgaaaca cgggtggccg atactaaaat aaactccacg agctgagcaa ctggcctaat
     6721 gtctgctacc aagacacatc aggcgctgac aatttcccat ttgattttat aagtatagtt
     6781 cgggtaaaaa attattcaat gttttcaagc tggttagtaa aaattagttt tctttcttat
     6841 gaatgaatgc gaaaactaca ccccaactca tttgtatttt agcgcgagta actaatttaa
     6901 aacgactgga tgataaggtt ttgctagtag taatgacggt tgaaagttgt ggcaggctat
     6961 tgagacgggt gttaagggct tcccaggttg caccgaataa ggtacttgaa ttaataaata
     7021 ttaaggtcgc ctcagataaa tcaacggtta gaaaatcacc caatataaat gagacttttt
     7081 tgctgctctc tgtatagcct tccatagccg ccaatttctg gagacgctcg caagcacatt
     7141 gatgtaagtt agggaacaat tcgataccta cacttttatg gactggatat accatagcgc
     7201 aggcgacaac agccttacca gttccagagc caagatcata aaaaacagta ttgtgatcaa
     7261 tattgaccat tgataataaa gcagtgaacg gtaaaaactc aatttcccca tacacatatt
     7321 ctattgcatc tcgattcttt cttgcttgtt gggaaatcat gaagccatta acatcaccgt
     7381 acaggagatg aaaaatttga gcatgatgct ttaaatttag ttttttttgc catcggttaa
     7441 tgcgcaactc ttttcttgcc ttatcaaaac aaagccaaaa aagaatgaga aacagaataa
     7501 ggcataaaaa aagcgttttt gacgatatga aatcaagcac agtattatcc tggtttcaga
     7561 ccatcttcat tcaagatttt tgccttttcc ttttgactta attttggcca tgcttgaaga
     7621 agaactttac cagcctggcg ttcactcatg gcttggtgat gcatttccca aggcatttca
     7681 tcattaaggt aaatgactat cacgaaggca gtgagtacgg tgattaacat ggcaaaaaga
     7741 cctactttga attgaagcat attaagcatt ttattgcttt tgtttatggc atcgtttgca
     7801 actcggtcga catggtcaca gctttcgtta gcgatgcgat gaaagtgttg aacatcgtat
     7861 ttagatagtt gcatagtcag tgtttgagtt gcttgaccag tatgcctttc gattctatca
     7921 atgcagtttt ctgttagggc attgagcctt tcttgttgtt gttggaagat ttgggtaaat
     7981 tgattgcaag ttctttctaa agaatgtaaa tgtgtttcgc cttgagccag tgccttttca
     8041 gcacttattt taaaatgggt tactcctgat tgagaaataa ggttttgcat tgaagttaat
     8101 tcattttgta gtgattgagc gatatgtaga actcgcccag cctgttgctc aatttcctct
     8161 cgccattcca tcattttcgc ttcgacaaac tcaaaatagg caataaattc tccagtatgt
     8221 ctcagcaact ctttaatgcg agccaagtca tcttcctgat aatgctccat gcctctctcc
     8281 ttactaaatt catagtcaga gaatcttcaa atagacctct cgcataatct atccttttca
     8341 ttttattgcc gcgttgcaaa cgtttctatg tttgcacttt gcctaaaaca aaataatcag
     8401 tttatacaag agagctaata atatcctttg atgcatcttg cctctttggc tgtgaattta
     8461 gaattatgat gttccatatt atattatatc cataacttta ttttggaaac ggcaattttt
     8521 gcctaaaatt ctcactcaat tttcgttatt agttccataa attctagatg ggtacagtat
     8581 agtcatgcct cataaagttg gcattattta tgcttgaatg taatatactc ggcatatact
     8641 ttctttatta ttaaaatcca ctgagacgag ttgtcggttt gcagaaataa tcgaagctca
     8701 atggcattat atgaaaatga gcggtggttt aaaaatgatg tggtcaagag aaacgccctg
     8761 cgttaaaagt ttcagtagac tgtaaaaact ttgttaatat ttccttaatc tggattgatt
     8821 agaatgaaga tataagttgg gataggagaa cattatgcca agagctaggt tctttaaagc
     8881 agaaactaat agttcgcaag tacagcaaag ctttattaat gtgggcggag agcagtttca
     8941 tgcggctgca gttgctttaa ttgattattt taagagatcc ccacgcatta atgatgatac
     9001 tttgaaaaaa atactggagc gtttctactt tcattttcca aaatacattc ccagccaacc
     9061 ttatttgacc ccctccgaac gcatgagtat gttgattaat ggggcacgga agtcagaaat
     9121 tgtcgaatgt tttgcttatg tattacgcca attggctata gatgagattt atgcaaaacc
     9181 tctcaattat cgtgatatct ttgctgaatt cgaccgcgac acgccaaaat cattgttacg
     9241 tcaaccatct actcctctcc cagccagtgc tttgagagcc ctttctcatg taattggtac
     9301 aaacatcact ttatctgttg tggaaaatgg caaggaattg aggaaacgtg aagtgtatgt
     9361 tggggataat ctgaatagtg caaaacccgg gataaccatt caggtccagc aaaataacag
     9421 gtatttccct gccgtgaaaa ataaaggtga ttttgtttat gtagggcaat tggcagtaag
     9481 cccccccaaa ccagtagaaa gctccaatac aagtgggaaa gatactatcg ctgatatggt
     9541 tgatttaatt gcgtctgata ataaaaaatt attacagacc tataaacaat ggcatcagaa
     9601 cttcctaacc atgcttaaat taggcgaaat aacaacccag aggatgatca atctttacat
     9661 caagttttta ccttcagaaa aaggcttagt tgttgactca gcagaatttt tttccaggtt
     9721 atcagaagat tctgacaagc cggttattcc tgattattcc ggtgaaaaca aggatggagt
     9781 caatcagtta ttggcttctg cgctggctgg atggattagt gccagacagg tcgatgttga
     9841 tcaattgttt gatcatttag aaaatcgacc ttcttcagta tccatgtctg ttggttaaag
     9901 tgtgttggca atccggatca ttttttcaaa gcggattgtc aacgatcatt ttttgatgaa
     9961 atagcaatac gatagttttt tatggctttc aattaaagaa agcccatttc tttcatccat
    10021 tcatcattgg caaatttgga catgtaatta cggatagagt cgggtaaaat caccagacat
    10081 ttttgtcctt tgcttaagga tttggctgct tgcaatgcgg cccacatcgc tgctccacat
    10141 gagccgccaa ttaacagtcc ttcttcttta atgaggcgtc tggcagtacg gaaagaatcc
    10201 gcatcattgg tttttatgta ggcatcaatc aacgtattat ccagcacatc agggaagaaa
    10261 tcataaccta tcccttcaac atgataagat ttgatttctg tgccgccacc gagaattgag
    10321 ccttcgggat ctgctccaat gattttgatg gcagggttaa attctttaag gcgtttggca
    10381 atgcctgtaa ttgttccgcc tgtcccaacg ccagcaacaa ccatatgtaa atcttttccg
    10441 aagtcgtcaa taatttcctg agcggtccca aaataatggg cattggggtt attggggttc
    10501 gcgtattgat caaggatatg ggagttaggg atttcagctt gtaatttttt agccagagaa
    10561 atatgactgt ctggatcatt atacgccgct tctgttggcg ttcgataaat gatggctccc
    10621 aggcgttcga gcacggattg cttttcctgg ctcatttttt ctggcatggt aataatcact
    10681 ttgtagccta atacagcgcc tgccagggct atacctatgc cggtgttccc ggaagtaggc
    10741 tcgatcaagg tatctcccgg tttaattctc ccttcctttt ctgcttttac taccatttca
    10801 tagccaatac gatcttttac ggagccgcca ggattaaaga attcgcattt ggcatacagc
    10861 tcacattcca gatcttttcc aagcctgtta atttttacaa cgggagtatg gcctatagtg
    10921 gccaggatgt tagggtaaat catggtagtc ctttgtcatg gtgaaatgag ttgattataa
    10981 gcggataagg taaaatagca aaatggtggt ttctacaatc aagttatttt ctcttattaa
    11041 cgggagttat agataagggt tatataagaa gcaatcgata tccagtattc gtttgggctg
    11101 aggagatgcg taagcatcgt ctcgaagcct tggtaccaaa catcaaattt caggcaaggc
    11161 ttcaagacag cctaaatgcc tcgtcagccc gaacgataca agataatgac ctatattaat
    11221 ccataactcg cgtttattgg aaagaaatgc aaatcatctt ggcagtttat atttgtgata
    11281 tcctccttat taatgcaggg atacttcgtt tttatggtta tttctccttt tttacattat
    11341 atttattgtg ggcgttggtt atttttgtaa aaacagaaaa acattactta ttaattgaga
    11401 tcaaaaaacg gatttctatc tgttttacaa aaatggcaaa aatccaaagt gttttcaaat
    11461 agttacaata attcatttca attgatattt tgtttaatta acgaatgcag gaagcttaat
    11521 tatgataaat tacaaaacga taaaatggag atatggcctc attgcgctct ttgctttagg
    11581 ggtatcagga tgttattttg acgggacaag ccctcaagaa agagggtatt cttaccagga
    11641 aaatagcatg ccatccagtg gaggacaaac caccgctagc aatcaatccc agccgcaaca
    11701 aaaaagctat gcgtcaaagg atacctctca aaaatctact cctgggccaa agcgaaccgc
    11761 tgcaccccaa ctcccggtaa ttcaataaaa agagttttac ttattatccc tcataatcaa
    11821 cttatatgag ggataactaa aacttgttca aaagattttc tcataggtat gaaatgccat
    11881 gatccttgat agttatcaaa gatggcagta gagaaattcc tccttgtttt ggttagatcc
    11941 ataatgttat tttttctgat atttgcgtgc cttgaaaaat tattttagaa tggcggccgt
    12001 ttagtagtct gaaatacagg agtagatgat ggataagtta aattgggagc agcctgaggt
    12061 ttgtgctata ccaatagaca gtgttagcgc tagcactgag gaagctgacg ttcaggacgg
    12121 cagtagtgaa agcggccttt cctcaagcag cagttcaggt ggttaaaaag aaaagggttc
    12181 tttataagta aagtacaaat tacccttttt ctatttttta gattcgccag caatcaagcc
    12241 tttaggataa ttaatggaat atggcaacct tattgatatt ttaaacacga atgaaaccgc
    12301 cttgacttca ttttcagcac ctgaaatcct aaagacagca caaagaatgc gcctgaccaa
    12361 ttcggtttat accattttaa gggagcacta tggtattcct gcttctcaac tcgctaactt
    12421 taagctcgtt ttaaaggaaa atagagacag ggccattagg aaattatttt tgtccatcaa
    12481 aatcaacgag ctttttcagc aatacaatat tccggttatt cacctgaaag gactaccact
    12541 ctcgcaaaaa ttgtttggag accctttgat tcgtcagtca tgcgacatcg atttatatat
    12601 ttctccccag cacttggaac aggcagatga agtgtttttt gaaaatggaa tcattcggga
    12661 taaatacagt ttcttacatg acgttaataa gctaaagaaa catcgagtac taaaggatgt
    12721 ttcgtacgtg gctgaaaaat atggtattaa aatcgaactc cactggcgtt tgtttagatg
    12781 gaaagataaa ttcaatcagc cttttgagtt ttattggaac aataaagtgc cgattttggt
    12841 ggggggaaaa aatttatttt ataccctgtc ccctattgac gagtatctgt atgtcgctgt
    12901 acatggagcg aatcatggat ttgaatcttt gcactggctt tatgattttg cccgttattc
    12961 caccttgctc agccaggatg acagcagaag aattgttgaa ttggttaata atctttccat
    13021 acagacagag tatcaaatgg cgacttattg tgcggagaga atatttccaa gtgtgtttac
    13081 agaaagcggt aatgcaggtt tactacccaa ctctttaacg agaaaagccc tgatttcttt
    13141 aatttaccat tcaaaaagca catatcgctc gaaaagaaga atattacgta tgatggcgaa
    13201 caaattttat tacatttaat gaaaagctta gagtagaaat ggagcttgct actaccggca
    13261 acgaatttat tagttttgct gttgcggtag ttacattttt ggctggttgg aaattaagca
    13321 gcacaaacat tagtagttat tttttatctt aggatatagc atgaatgctt tgtatggagt
    13381 tctgcgtttt gacgggcaac catttgatcg agagttatta gaacgagcca aatttaatag
    13441 cccggtttgg agagcggatc atagcgaact ttattataat gatttttatg ctacggcgag
    13501 cacacagcgc tttattactc ccgaatgcgc cttacaaaca gctccatatc atgataagga
    13561 atcgggtgta attgccaacg gcgatcttta tctaactaat cattccagtt tggcaaattg
    13621 gttaggagtt agctccaaaa attcggattt atacctggca acccgtgcat atttaaaatg
    13681 gggtgaaaaa tgtgtgaatt atttagcagg gaattttttc cttgtatttt atgacttacg
    13741 caaaaaacgg ctgtttattg ccattgatca ttttaatttt tgttgctgtt actatctgtt
    13801 tgtgcctggt aaatacttta tatttagcaa tacattcaat acattcaaag tttttcatga
    13861 ctccttagcg gtgaatcatg gcgctttcca taagcttgcg gcaaaacaga aaataggtca
    13921 ggaaacttgc ctgaaatcgg ttaaaaaact gacggctgcc agatatatga ttgttagcga
    13981 ccaaaattta caagagcaac aatactggga cctccgtaag acacaacaga caggacaatg
    14041 ccaatcgcgt gaagactact atagtgcatt cagaaatgag attcagcaaa gtatcagaca
    14101 aagtatccgg actgacgcag aaatcatggc ccattacagc ggtggtttag actcttcatc
    14161 cattgcttcg attgctgcct tgcaacttgc caaagaaaat aaaactctta tggcatatac
    14221 cgcgattcct aaatatttgt cgggcgatag tcatcggaaa ggatgtaaat atcatgaatt
    14281 agatcgggtg caaagcgcat gccgttacta tgacaatatt gaaacaatag tttatcaaag
    14341 ctctgaagac ttgagtattt ttgataaact tcaagtcttt tatccttatt tagatcagcc
    14401 attcaaagcg gtattcaata tggaatggat attggcagga ttggaaatgg cagcagccaa
    14461 aaactgcagg attcttttaa atggtgaaca ggggaatgga tccatttcat ggaagggagt
    14521 gacattgggc aactatctga atcagcgtgt aaaatttacg cggcatcttt taaaatacca
    14581 tatgaaatta ggcaagcaat ttataaaatc tggctcgcta aacctgaaaa aaaatcgtac
    14641 tgatctcaat gagatgaaaa aaaatttttt agagccccat tattctctcc tgagaggtaa
    14701 gaacaggttg gaaacaagaa gttacatgcg ttccatctat ttgtggtttg gggtagaggt
    14761 gattgaccca acaagtaacc tggatctggt ccagttttgc tatgatcttc ctgaatgggt
    14821 ttatcatgga ggtaacaaat attttgaaat caggcgattg cctaaattaa aaaggcgctt
    14881 attggtgaga gatgctctgg aagggcttgt gccccctgag atccgccata atccctatcg
    14941 cggtgaacag gcagcagatt ggtactatca attcaataaa tatggtcatg cctggggaag
    15001 gcttttggca gatattgcag agaaacaccc tctcatttgg gaaatttatc ctaaaaaata
    15061 ccttttggat ttatttgcta atcatcccat tagtccgcaa tcaagaaatt ttcaagacgt
    15121 taatatgcat ttggtcccag ctttaagcac agcatttttt atcaactacc taaatgatct
    15181 gtattgatta ttacttactg gagataatct cgatgagtaa ccatttacaa caacacaaat
    15241 atcaattcga atataaaacc aatacaatat tagaacgcta tgacttggtg tttttttcca
    15301 gcgttgatga ggacttgatc ctgcttgatg aagaagcgag tcattatttg gtagttaatt
    15361 ccgtaggtta caaaatatgg aatttgctgg aatcaaaaaa aacggtgagc caacttgttt
    15421 ctgggcttat ggaaatatat aaggttgatt tcgatacctg ctttaatgat attaaagcgt
    15481 ttatacaaca aatggttcaa cataaattaa tcaaagtctg ttcatagata aagaatttat
    15541 aatcattcgc tgtcatagtt tttaagcaga attggataat acaattgact gaaataaaga
    15601 aaataagata tgcccatctt tttctggttt tattggacaa gggaaaaaca cgagttcatg
    15661 ttgctgtgct tattgtttat tacctgatag tatcaagaat cattacctgg ttatatccgt
    15721 ttaacagata tgccaagtcg tttgggcata aacacgccga aactttgtat gaagacttcg
    15781 accatttgta ttggctaaaa tgttgccaaa aactgtttcc taaaataaat agattgttgc
    15841 catggaaaag tgcctgttta cagcaaagtg ttgcagcatt tttgctatta cgtcgcaaaa
    15901 atatgcccac aacgatttat tttggtatga agcgagaagg agatgaggtc atagggcacg
    15961 tatggttgcg gtgcggtaaa cactatatca ctggaggaaa cggcgtgggt tatactattt
    16021 tttgtactta tgcattagac cgggtaaaaa attaagtgtt gttttatggc tgaattttat
    16081 aaaaaatatg cgggaaaata taaagacttt ctcgatttca tcaggttttt atatgaatat
    16141 tcgccattcc agcttgtgtt ttcacaagca atggtaattc tttcaagctt tctgggcggt
    16201 attggagtgg tctcattggt gccaatgtta agctatagtg gttggctagc ccaaaataag
    16261 tccggctctg atctcaattg gttatccagt ttatggcaac aattgccgat gcacgatggc
    16321 caaattccaa tcacttttgt ctt
//
LOCUS       DACTJE010000061        16323 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.061, whole genome shotgun sequence.
ACCESSION   DACTJE010000061 DACTJE010000000
VERSION     DACTJE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16323
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.061"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..3804
                     /locus_tag="I9760_001526"
     CDS             <1..3804
                     /locus_tag="I9760_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HAT5043298.1"
                     /translation="VASNITRSGLANEGADTNLMLILDTSGSMAGSGIQTLINSTLEL
                     LERYEALGNVKVRIVTFNTSATAIGSVWMTVDAAKNALLGLTAGGNTNFDAALITAMN
                     AFNSGTVDGADGRIGGAQNVSYFISDGNPTVNQDWPSIPGTLAQNGIQPSEQAAWEAF
                     LQDTNGASAGGEKIRSYALGMGTGITVSNLEPIGFNGVNGTDIPAVVVTDISQLDSVL
                     ASTITASPISGTLISDPNPGVNFGADGGHVQSVTVNGITYTVNANLSSLSTSGSATQG
                     SNTHSYDNSTHVLTVTISTGESLTIDMDNGSYTFTPPANITSSINRIINYVLVDGDGD
                     TAAATITFNIDISGFNDPLVVRDDLVLTNQPAVSGQDVISIPTWALLANDTGGSGVSS
                     VSSVTQPVAEGTVVLNAGSVAFTEESNSAADGTYNGSTSDPFRYTNTTGSQSATAKVD
                     LDRTQAGENTLDGTYRSEILLGRDTSNDTIIGNDGNDILIGLGGNDTLSGGNGDDILA
                     GGAGNDTLDGGAGNDTATYIDATAGVTVSLNTSSAQNTVGAGTDTLTNIENLTGSKFN
                     DTLTGNNSANTIKGLEGNDTISGLGGNDTLDGGSGDDVIIGGLGDDILTGGSGADQFV
                     LVKGQGGASAGAAPIDTITDFEVNIDKIVINGNNITGVSVSTPVSNTYTITVNYSDAA
                     TEHFKVTLSNSALLNDSGKTHLNGVVISGGTATIDGTIVGAVLYMDLNANNQEDEGER
                     LGITDQYGHVEWVVDISKFDVNGDGQYVIGEARAVQTGGFDIDTGLSYEINLYGPVGS
                     AVISPLTSLLQAQLEAGMDYETANAALVARLGLPEGTQIISFNPITGSGEVLAQNAGV
                     MTAAIQFAEIAAIHYSTDEGHVSFAVFEAISKALLELPEGVVADFGDEGFLQSISNYL
                     GLDALVTSDMIDYMATSQKALEASILTLAPGEDALTAISKIQHVTQGIYAQAIEMALI
                     GYLPLDALKNMSAVLKAYADGDITDEQLNSFEDKLSAVIVNWEDNNVTDTSHQATLQT
                     ERTVDDSSVKSEVNHEMAQEVNAVINDFMDEHNISLYYYDQQAQMPQEAQEQQPTEHQ
                     AEETVMHDDVVQDLLLDDHSLTMLANNPQLNGGNGNDVLHGTTGNDFIRGGQGNDTMT
                     GGGGVDTFFWLSGDDDGGVDTITDFKANPVDQSSDASVLNLSDLLSDADLETNSLDNY
                     LNVSTTEEGDTAIKVDPNGNGNFDAPAQTIILEDVDLTAVFATNNSHDIVNQMIANGN
                     LIVEQ"
     gene            3983..4582
                     /gene="wrbA"
                     /locus_tag="I9760_001527"
     CDS             3983..4582
                     /gene="wrbA"
                     /locus_tag="I9760_001527"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAT5043299.1"
                     /translation="MSNPYILVLYYSRTGSTAQLAQYIARGVESVSGLEARLRTVPPV
                     STTCEAVDKPVPDAGAIYVSLDDLRHCNGLALGSPTRFGNMAAPMKYFWDGTTSLWLG
                     GDLVNKPACVFSSSASMHGGQETTLISMMIPLLHHGMILLGVPYTEPALTSTQSGGTP
                     YGVTHVAGGDNENPLSQDEISLAKALGERLGRVAMGLKV"
     gene            5076..5426
                     /locus_tag="I9760_001528"
     CDS             5076..5426
                     /locus_tag="I9760_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAT5043300.1"
                     /translation="MVPADAKLLKQAKSLRKRSTDAENCLWYYLRAHRLKGYKFKRQV
                     PIGNYIVDFVCIQRKLIVELDGGQHLLNKEYDKDRSAYFSTLSYKVLRFWNDDVLLRT
                     GDVLESIVRCLEES"
     gene            5482..6264
                     /gene="xth"
                     /locus_tag="I9760_001529"
     CDS             5482..6264
                     /gene="xth"
                     /locus_tag="I9760_001529"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAT5043301.1"
                     /translation="MKVISFNANGIRSSARNGFYDWLVGQDVDFVCIQETKSQVEQLI
                     PEELYFPRDYFCDYFDAEKKGYSGVAIYSKYKPVRIVKGLGFDYCDKEGRYIQFDYPK
                     LSVISLYLPSGTSGEDRQEVKYAFLEQFARHLMNLKNEGRELIICGDYNIAHKKIDLK
                     NWRGNQKNSGFLPEERAWMDELFGSMGFVDAFRVHNQEEEQYTWWSYRARAWEKNVGW
                     RIDYQVITPGLTEYVADSRIFREARFSDHAPLMIEYKGDWCV"
     gene            6257..7027
                     /gene="xth"
                     /locus_tag="I9760_001530"
     CDS             6257..7027
                     /gene="xth"
                     /locus_tag="I9760_001530"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAT5043302.1"
                     /translation="MFKLASWNVNSLKVRLEQVLQWLESSQVDLLAMQETKLTDENFP
                     VASFTDKGYHVIFSGQKTYNGVAIISRYPMTDIQTDIPELEDPQRRILAATVKGIRLI
                     NLYVPNGGELTSDKYQYKLDWLNKVTRFIENQLSIYSKVAVVGDFNIAPEDRDVHDPV
                     EWEGCVLVSPAERQAFMKILELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDH
                     VLLSHELNALCRQSKIDKEPRKAERPSDHAPVWVELEL"
     gene            7164..7391
                     /gene="rpmE"
                     /locus_tag="I9760_001531"
     CDS             7164..7391
                     /gene="rpmE"
                     /locus_tag="I9760_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAT5043303.1"
                     /translation="MKASVHPDYQTVKVTCSCGEVFETRSTLCKDLNIEVCSMCHPFY
                     TGKQKLVDTGGRVQKFRDRYNMRTGQAKSKE"
     gene            7518..8753
                     /locus_tag="I9760_001532"
     CDS             7518..8753
                     /locus_tag="I9760_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAT5043304.1"
                     /translation="MDNEVIKQRALDYHEFPVPGKLSVHLTKSTNSQDDLSLAYTPGV
                     AAPVLAIAEAPENAYRFTSKGNLVAVMTNGTAVLGLGNLGPLASKPVMEGKAVLFKRF
                     ADLDVFDIEIDAEDPQSFIATAKRIAPTFGGINLEDIKAPECFEIEQALIEQLNIPVF
                     HDDQHGTAIVVAAGLLNALELQNKKLSDIKIVCMGAGAAGIASMRLLVALGAPKSNML
                     LLDTKGVIHSGREDLNPYKFAFARNTSCRTLEDALVDADVFIGVARPDLLNAHLLSLM
                     APNPVIFALSNPDPEIKPELAHSVRKDLVMATGRSDYPNQVNNVLCFPYIFRGALDVR
                     ATCINQAMQIAAVEAIRQLVHEPVPQVVKDNYPGVVNWDFGPDYIIPKPIDPRLKERV
                     PAAVAKAAIASGASQFNNL"
     gene            8965..10257
                     /locus_tag="I9760_001533"
     CDS             8965..10257
                     /locus_tag="I9760_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5043305.1"
                     /translation="MHTENRTNYAMIGWLLCGLGAIFYSYEYLLRIAPSVMENALRTH
                     FNLSASGFGFLSSIYYLAYVPMQLPVGVLLDRYGPKRLLTMACLVCVIGTYMFTGTTI
                     FWVAATGRFLVGFGSAFAFVGVLKIATLWLPENRLAMISGMTSALGPIGAMLGDNFLE
                     LFIVKMGWVKTLNMTAAFGIVLTLVLWVGIRDRKGQHRQSGTVPTLKKGLVDLGIIIT
                     SRQIWVNGMYGCLVYLPTTVFAELWGIPYLHHAHGLTTQAAGLANSVLFLGFIIGAPI
                     MGYISDRLYRRKFPMLIGASGAAIVMMMILYLPGLNESNIQALMFLLGLLYSAQAIVF
                     AVGRELSPGEAAGTAMAVTNMIVMLGAMFLQPLVGHLLDFSLSTHLSDTTGIVVDNMS
                     KLYTVDDYQFALSIIPLGILIAAILTFFLKETHAKAPK"
     gene            10238..11515
                     /locus_tag="I9760_001534"
     CDS             10238..11515
                     /locus_tag="I9760_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5043306.1"
                     /translation="MQKHLNNMTTKKQISYGVLVCFIGAVFYCYEFILRIVPGALQTE
                     LSTALGHISATTFGQISALYYFAYSPMQMPVGMLMDRYGPRRLLTFACLCCALGSWMF
                     TLTWSIVLVGIGRFLVGFGSSFAFVGVLSLALHWLPRRYFSLVAGLITTLGMLGLVYG
                     EIKITEWSEVIGWDHVLMHIAMIGSALCILTFFVVRDGPEGYQPNKYPWPEFFHNVLK
                     VLTSHEVWLIGFVGACLYTSLSVFGELWGKTYLEQAHHLTKVEAAKTVSAVFLGWAVG
                     APLAGYISDITRRRLLPLVIGAIVSLICISFVLYCPGLSYLTLNILLFLYGVFSATEI
                     IVFIMAKECSGAKLSGTVFAVTNMIISLAGAIFQPLVGKLLDTFGDSGIVAGEHIYTV
                     DDYQVALSILPISLLLVTILAFFIKDKDHSNNS"
     gene            complement(11546..12364)
                     /locus_tag="I9760_001535"
     CDS             complement(11546..12364)
                     /locus_tag="I9760_001535"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HAT5043307.1"
                     /translation="MVKIRPFLKWAGSKYNCLNEIISSLPTGQRLIEPFAGSGVVFMN
                     TSYSSYLLAESNSDLVNIYRILQLNGESFIDYCQQYFTPEANCKEKYYELRSEFNNLM
                     DSNQKSAVFLYLNRHGYNGLCRYNSKGKYNVPFGLYEKPYFPRKEMMLFYKKCLSAQF
                     IHGDFRNTFALAEPGDVIYCDPPYVPLTENNQTLPYNQKLFSLEDQIELAELAQETAS
                     KGIPVIISNHDTEFTRHHYKKAQIKSFYVPRWISCRSQMRLPVKELVAVFNCPS"
     gene            complement(12535..13731)
                     /locus_tag="I9760_001536"
     CDS             complement(12535..13731)
                     /locus_tag="I9760_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan/tyrosine permease"
                     /protein_id="HAT5043308.1"
                     /translation="MINSKLIGGILLIVGTSIGGGMLALPVSTAEVGFTNSLFFLFFC
                     WLVMTAGALLILEVNLRLPAGSNMISMAKSTLGLPGQVIAWITYLLLLYTLLAAYISG
                     GSDVFSSLLHKAHIDLPNWITSVIFTAMFSLIVYSGIRSVDYVNRGLMFGKLGVYILL
                     VIIISPHVSTAGLTGGSAKAFTGTLMILVTSFGFASIVPSLRDYFNDDIRSLRRVILF
                     GSLIPLFCYIVWDAVIMGVISREGENGLLSLMNSDHATSGLTEALSQSVSSQWISGFF
                     GFFTSICMVTAFLGVSLGLFDFLADGLKLKKSGNQGKYTLALTFIPPLAVVLFNPGIY
                     LHALSYAGVCCVILLLLLPSIMAWKGRQTCSQSPSETHLIPGGNPALSLIGFIAIGLL
                     IIAIMY"
     gene            complement(13932..15122)
                     /locus_tag="I9760_001537"
     CDS             complement(13932..15122)
                     /locus_tag="I9760_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan/tyrosine permease"
                     /protein_id="HAT5043309.1"
                     /translation="MRSRFIGGILLIVGTSIGGGMLALPVANAATGFWQSSLFLLICW
                     AFMTFGAFFILEANLYLSRGKHMVSMAKATLGDYGLLTAWISYLFLLYTLLSAYISGG
                     ADVLNSLLSKVNWHLSDWQASSLFTLIFGLVVYGGIHSVDVANRALMFGKLAVYCILV
                     ILIAPHIEFHYFQHGDYRQIGGAIMILITSFGFAIIVPNLRDYFDDDIKTLKKVIFIG
                     SLIPLICYIAWDAVIIGSLPSAGTSGLESLMQNPRTTSALASLLTDKVENTTISALFN
                     FFTSICMLTAFLGVSLCLFSFLADGLKLRERGSHGIGLFVLTFLPPLLIVIYYPGAYI
                     YALRYAGIFCIILLLLLPALMCYFGRKRYKSPFWVPGGKISQFAVIIISVVLLAHEFI
                     ALFK"
     gene            complement(15133..15825)
                     /locus_tag="I9760_001538"
     CDS             complement(15133..15825)
                     /locus_tag="I9760_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAT5043310.1"
                     /translation="MSRKLAKTRILGYGLMICFLAGCFHPPYNNFQPDRRAVKRVGVD
                     TGIGAVAGVIASGTASGTLIGAAAGGTVGLVASIYRDSKRKIIRDLQKQDIQYVEYGD
                     TRTLIIPTDKYFMFSSPRLNEICYPGLNNVIRLLNFYPQSTIYVAGFTDNVGSRSHKR
                     KLSQAQAETMMTFLWANGIAAKRLKAEGYGDKNAISDNAIIHGSAQNRRIEIQWFTSP
                     AQPPQPQMAYVK"
BASE COUNT         4836 a         2900 c         3611 g         4976 t
ORIGIN      
        1 gtggcatcaa atattacaag atcaggatta gcaaatgagg gtgctgatac aaaccttatg
       61 ctaattcttg atacttcagg aagtatggcg ggttcaggaa ttcaaacctt aattaattcg
      121 actttggaat tacttgagcg ttatgaggcg cttggtaacg taaaagtacg tattgtaact
      181 ttcaacacaa gtgcaacggc tattggctct gtttggatga ccgtagatgc agctaaaaat
      241 gcactccttg gacttactgc aggaggcaat acaaattttg atgcggcgct tattaccgct
      301 atgaatgcat ttaattctgg tactgttgat ggagctgatg gaagaatagg gggagcacaa
      361 aatgtgtcgt attttatctc tgatggtaat ccaacagtta atcaagattg gccaagtatt
      421 ccaggtacat tagcacaaaa tgggattcag ccaagtgagc aagctgcatg ggaagctttc
      481 ctgcaagaca cgaatggtgc atctgctggt ggcgaaaaaa ttagatctta tgccttaggc
      541 atgggtacgg ggattactgt cagcaatctt gagcctatag gatttaatgg ggtaaatggt
      601 acagatattc ctgctgttgt cgttacagat attagtcaac tagacagtgt attagcaagt
      661 acaattactg caagcccaat tagcggtact ttgatctcgg atcctaaccc tggtgttaac
      721 tttggagctg atggagggca cgttcaatct gttacagtga atggtataac ttatactgtt
      781 aatgcaaatc ttagttctct ttctacaagt ggttctgcta ctcaaggatc aaatactcat
      841 tcctatgata attcaacgca tgtgttaaca gtaacaatta gcacgggtga atcgcttact
      901 atagacatgg ataatggtag ttatacattt acaccaccag caaatattac cagctcaata
      961 aaccgcataa tcaactatgt tctagttgat ggtgatggtg atacggctgc tgcgacaatt
     1021 acttttaaca ttgatatttc cggattcaat gatccgttgg tagtacgtga tgatttagtt
     1081 cttaccaatc aacccgctgt aagtggtcaa gatgtcattt ctataccgac atgggcatta
     1141 ttagccaatg atacgggtgg aagtggcgtg tcttcagtca gcagtgtgac acaaccagtt
     1201 gctgaaggta ctgtagttct caatgctggt tctgtagctt ttaccgaaga atctaatagt
     1261 gcagccgatg gtacctacaa tggaagtaca tctgatcctt ttaggtacac taatacgaca
     1321 ggttctcaat cagcaaccgc taaggttgat ctggatcgta cacaagcagg ggaaaatacg
     1381 ttagatggaa cctacagaag cgagatttta ttaggtagag atacttcgaa tgacacaatt
     1441 attggtaatg atggtaatga tatactgatt ggattaggtg ggaatgatac tttaagtggt
     1501 ggtaatggtg atgatattct tgctggtggc gctggtaatg atacgttaga tggtggtgca
     1561 ggcaatgata cagctactta cattgacgcc actgcaggtg taactgtttc cttaaatacc
     1621 agttctgcac aaaatactgt tggtgcagga acggatacac ttaccaatat agaaaatcta
     1681 accggttcca aatttaatga tactctaact ggaaataata gtgccaatac aatcaaagga
     1741 ttggagggta atgatacaat aagtggatta ggtggaaatg atactttaga tggcggttcc
     1801 ggagacgatg tcattattgg tggattgggt gatgatatac tcaccggcgg atcaggggct
     1861 gatcaatttg tgcttgtgaa agggcaaggt ggtgctagtg caggcgcagc cccaatcgat
     1921 actattacag actttgaagt gaacattgat aagattgtta ttaacggtaa taacattact
     1981 ggtgtcagtg tatccactcc ggtttctaac acttatacta taacggttaa ttattctgat
     2041 gctgctacgg aacactttaa agtgacatta tccaatagtg ctttattgaa tgattctgga
     2101 aaaactcatt tgaatggagt ggttataagt ggcggaacgg ctaccattga tggaacaatc
     2161 gttggtgcgg ttttatatat ggatctgaat gccaataatc aggaagatga aggcgaacgg
     2221 cttggcatca ctgaccagta tggccatgtg gaatgggtcg ttgatatctc taaattcgat
     2281 gtcaacggtg atggtcagta tgttataggc gaggctcgtg ctgttcaaac cggtggtttt
     2341 gatatagata caggtcttag ctatgaaata aatctttacg ggccagtagg ttcagcagtt
     2401 atatcgccct tgacaagtct cttgcaagcg cagctagaag ccggtatgga ttatgagaca
     2461 gctaatgctg cgttggttgc tcgcttaggg ttaccagagg gaacacaaat tattagtttc
     2521 aatccgatta ctggtagtgg agaagtcctg gctcaaaatg caggtgtaat gacagctgcc
     2581 attcagtttg ctgaaatagc cgcaatacac tattcaaccg atgaaggtca tgtttcattc
     2641 gctgtctttg aggcaattag caaggcattg cttgaacttc ctgaaggggt agtcgctgat
     2701 tttggcgatg agggtttctt gcagtccatt tcaaattatc tgggattaga tgctctggtt
     2761 acatctgata tgattgacta tatggcaacc agccagaaag cgttagaggc cagtattttg
     2821 actcttgcgc ccggagaaga tgctctgacg gccatcagca agatacagca tgtgacccaa
     2881 ggtatatatg cccaggctat tgaaatggca ttgatagggt atttgccatt agatgctctt
     2941 aaaaacatga gtgctgttct taaggcttat gctgatggag atattacgga tgaacaactc
     3001 aactcctttg aagataagct ttctgctgtg attgtgaatt gggaagataa caatgttaca
     3061 gacacatctc atcaggctac attgcaaaca gagcgtactg ttgatgactc ttcagtcaaa
     3121 tcagaggtga atcatgaaat ggcgcaagaa gttaatgccg tgattaatga ttttatggat
     3181 gaacataata tttctttgta ctattatgat caacaggctc aaatgcctca agaagcacaa
     3241 gaacaacaac caacagagca tcaggctgaa gagacggtca tgcatgacga cgttgttcaa
     3301 gatttgttgc ttgatgatca tagccttacc atgttggcta ataatcccca attaaacggt
     3361 ggtaatggca atgatgttct gcatggaact accggaaacg attttataag aggcggtcaa
     3421 ggaaatgata cgatgacagg tggtggcggt gttgatacct tcttctggtt aagtggcgac
     3481 gatgatggtg gtgtggatac tattaccgat ttcaaagcaa atcctgttga tcaatcatca
     3541 gatgccagcg tccttaactt aagtgatttg ctatctgatg cggatctgga aaccaatagt
     3601 ttggataatt atctgaatgt ttctaccaca gaagagggtg acacagccat taaagtagat
     3661 cctaatggca atggaaattt tgacgctcca gcgcaaacga ttatattaga agatgttgat
     3721 ttaacagccg ttttcgctac taacaattcg catgatattg tgaatcaaat gatcgctaat
     3781 ggaaacttga tagtagaaca gtaagatgaa ctcattacat tggtgaatat ggaattcacc
     3841 aatgttttat taattggaaa atatcctcgc gacatttttc tcgctgctat atactcatgc
     3901 gttcattcga taataagcct tgcaattcat ttcacgttat taaaatgatg aaaaaaggca
     3961 aaaaacgcta tggagcagta tcatgtctaa tccttatatc ttggttttat actattctcg
     4021 cactggctca acggcacaat tagctcaata tattgctcgt ggggtggaaa gtgtgtctgg
     4081 gcttgaagcc aggttacgca ctgtaccccc tgtatctacc acttgcgaag cggtggataa
     4141 gccggttccc gatgctggcg caatttatgt ttccttggat gatttgcgtc attgtaatgg
     4201 gttggcttta ggcagtccta ctcgctttgg taatatggct gctccaatga agtatttttg
     4261 ggatggaact acttctttgt ggctgggtgg ggatctggtg aacaagcctg cttgtgtgtt
     4321 ttcttcttca gccagtatgc atggtggaca ggagactacc ttaatctcca tgatgattcc
     4381 tttgttacat catggcatga ttttattagg tgttccttat actgaacccg ccctaaccag
     4441 tacgcagtct ggtggtacgc cttatggtgt aacccatgtg gctggtggtg ataatgagaa
     4501 tccattaagt caagatgaga tttcgttggc taaagctctg ggggaaagat tggggcgtgt
     4561 agcaatggga ctgaaggttt gatgagaggg ttttaggggt tgattacccc tctcccttac
     4621 cctctcccca aaggggcgag ggaatgatta attaaactat atccttctcc aaataggcga
     4681 gggaatgatt aattaaacta tatcctttct ccaaataggc gagggaatga ttaattaaac
     4741 tatatctttt ctccaaatag gcgagggaat gattaattaa actatatctt ctctccaaat
     4801 aggcgaggga atgattaatt aaactatatc ttctctccaa atagatgagg gaatgattaa
     4861 ttaaaactag agttttctaa attgatggct atgcgagttc attgatttta gagtgataaa
     4921 ttttttgttt atgagcaatg atgtaatttg gtatttttaa agaaaattgt agtaattggc
     4981 gctgatattt aaacaaaaac tccctctccc gcgaaagaaa ttgtataaaa aatttgggta
     5041 tgattcgcgg gagaggggtg gggagagggg aataagtggt tccagctgat gctaaattat
     5101 tgaaacaagc aaaatctctc cgtaaaagaa gcactgatgc tgaaaattgt ttatggtact
     5161 atctaagagc acatcgatta aaaggataca aatttaaaag acaagttcct attggaaatt
     5221 atattgttga ttttgtttgt attcagagaa aactcatcgt tgaattggat ggtgggcagc
     5281 atcttttaaa taaagagtat gataaagacc ggagcgccta ttttagcaca ttaagttata
     5341 aggttttgag gttttggaat gatgacgtgt tattgagaac tggagacgta cttgagagta
     5401 ttgtcagatg tttagaagaa tcataaattt ggtaggatta atatttaacc gttcagtaat
     5461 gacaacggag ttgcctaagt aatgaaagta attagtttta acgccaatgg catacgctct
     5521 tctgcccgta atggatttta tgattggctt gtagggcagg atgttgattt tgtatgtatt
     5581 caggaaacga aatcgcaagt agagcaatta atcccggaag aactttattt tccaagggat
     5641 tatttttgtg attattttga tgctgaaaag aagggctaca gtggggttgc catttattca
     5701 aaatataaac ccgtgcgtat tgtgaagggg ttggggtttg attactgtga taaagaaggg
     5761 cgttatatcc aatttgatta ccctaaatta agtgtgattt ctctttattt accttctgga
     5821 acaagtggtg aggatcgaca agaagtgaaa tatgcttttc tggagcaatt tgccagacac
     5881 ttgatgaatt taaaaaatga gggaagagag ttaattattt gtggtgatta caacatcgcg
     5941 cataaaaaga tagatttaaa aaattggcga ggaaaccaga aaaactcggg atttttaccc
     6001 gaagaacgtg cctggatgga tgagttattt ggttctatgg ggtttgtaga tgcctttagg
     6061 gtacataatc aggaagaaga gcaatatacc tggtggtctt atcgagctag agcctgggaa
     6121 aagaatgtcg gttggcggat tgattatcag gtgattacgc ctggtctgac tgaatatgtc
     6181 gcggacagcc gaatttttcg cgaagcacgt ttttcggatc atgccccttt aatgattgaa
     6241 tacaagggag actggtgtgt ttaaactcgc tagctggaat gttaattcat taaaagtccg
     6301 tctggaacag gttttgcaat ggcttgagtc ttcacaagtt gatctgcttg ccatgcagga
     6361 gactaaactg actgatgaga actttcctgt tgcttcattt acagacaaag ggtaccacgt
     6421 cattttttca ggtcagaaaa cttataacgg ggtggccatt attagtcgat atcctatgac
     6481 agatattcaa accgatattc cagaattgga agatccccag cggcgtatcc tggctgctac
     6541 ggtaaaagga atcagattaa ttaatcttta tgtgcccaat ggtggagaac tgacttcaga
     6601 caagtaccaa tataaactgg attggttaaa caaagtaact cgtttcattg aaaatcagtt
     6661 aagcatttat tctaaagtag cagttgtggg tgattttaat attgcgcccg aagataggga
     6721 tgtccatgat ccagttgagt gggagggttg tgttttggtc agtcctgctg aaaggcaagc
     6781 attcatgaag atacttgaat tgggtttaca tgacagtttc cgtaattttg ctcaggaaga
     6841 aaaagcgttt agctggtggg attatagagc cgcagcattc cgtcgtaatc gtggcttgcg
     6901 cattgatcat gtgctattga gccatgaatt aaacgcttta tgccgacaat ccaaaatcga
     6961 taaagaacca aggaaagcag agaggccttc cgatcatgcg ccggtttggg ttgagttaga
     7021 attataaatg tctgaggtgt ttgagaaaaa attgctttat gagatcattt ctgctattgt
     7081 tttgactcat ttgtgtagag tatggtgctt ggcaatagtg tccaggtggc gatacttatt
     7141 tgaaactaga agagagtggt attatgaaag catcagttca tcctgattat caaacagtta
     7201 aagtaacatg cagctgtggt gaagtatttg aaactcgttc tactttgtgt aaagatttga
     7261 acatagaagt ttgttcaatg tgccatcctt tttatacagg aaaacaaaag ctggttgata
     7321 ctggtggccg tgttcagaaa ttccgtgaca ggtataatat gcgtactggg caagccaaat
     7381 caaaagaata atattttaat ttttgagttt ttaacaaagg cacaaatttt ttgtgccttt
     7441 tctctaagag aggattaaaa attcaaattt ttgatatgat gttttcaccg cgtgatctga
     7501 aatagagagt atgcactttg gataatgaag taataaagca gcgggcattg gattatcatg
     7561 agttccctgt tccagggaaa ctctctgttc atcttacaaa atcgactaat tcccaagacg
     7621 atttgtcttt ggcctataca cctggtgttg ctgctccagt tttagccata gctgaagcgc
     7681 ccgaaaatgc ttatcgtttt accagcaaag gcaatctggt tgcggtgatg accaatggca
     7741 ctgcagtgtt aggtttggga aatcttgggc ctttagcgag taagcctgtg atggaaggca
     7801 aagcggtatt atttaaacga tttgccgatc ttgatgtctt tgatatagaa atcgatgcag
     7861 aggatcctca gtcttttatt gctactgcaa aacgcattgc cccaactttt ggcggcataa
     7921 atcttgaaga catcaaagcc ccggagtgtt ttgaaataga acaagctttg attgagcagt
     7981 taaatattcc tgtattccat gatgatcagc atggtactgc gattgtagtt gcagctggtt
     8041 tgttaaatgc tttggaacta cagaacaaga aattaagtga cattaaaatt gtctgcatgg
     8101 gtgctggagc tgctggtatt gcttctatgc gactccttgt ggcattaggg gccccaaaaa
     8161 gtaatatgct tcttctggac acgaagggag ttattcattc aggccgcgag gatttaaatc
     8221 cttataagtt tgcttttgcc agaaatacat cctgtcgtac acttgaagat gctttggtcg
     8281 atgccgatgt gtttattggc gttgcaaggc cagatttgct gaatgcgcat ttattaagtt
     8341 tgatggcgcc caatccagtg atttttgctt tatctaaccc tgatcctgaa atcaaacccg
     8401 aattggctca tagtgtacgt aaagatctgg tgatggcaac aggacgaagt gattatccta
     8461 atcaagttaa taacgtatta tgttttcctt atatttttcg tggtgcgcta gatgttcgtg
     8521 ccacttgcat taaccaggcc atgcaaattg cagctgttga agccattcgt caattggtgc
     8581 atgagcctgt tcctcaggta gtgaaggaca attacccagg agttgtgaat tgggattttg
     8641 gacctgacta tattattcca aagcctatcg atcctcgctt aaaagagagg gttcctgctg
     8701 ctgtagcgaa agctgcaata gcgagtggcg cgagtcaatt taataattta taaggtaata
     8761 aattcacctt gtagaagtaa gcagtttagg tcttaatagc gtttaaacga aggatatctt
     8821 ggaaaaattc tttgtaaagc caccagctgg tttgcagaga gattgcaaga tgagctttta
     8881 tgcgcttata agatatgctg ggatgcagaa atgggaacta agacaggatt taaaaatact
     8941 tgactaattg ttaaggagat aattttgcat actgaaaacc gaactaatta tgcaatgata
     9001 ggatggctac tttgtgggct gggagcaata ttttatagtt atgaatattt attacgcatc
     9061 gcaccaagcg taatggaaaa cgcgttaagg actcatttta atttatcggc gtctggtttt
     9121 ggcttccttt cttccattta ttatctagct tatgttccta tgcaattgcc agttggggtt
     9181 ttattggaca ggtatgggcc aaaaagacta ctaacaatgg cttgcttggt atgcgttatt
     9241 ggaacgtata tgtttacggg gacaactatt ttttgggtag ccgcaacagg tcgttttcta
     9301 gttggatttg gctctgcttt cgccttcgtc ggtgttttaa agatcgcaac tctttggctg
     9361 cctgaaaatc gattggctat gatctctggt atgacatcgg cactaggccc catcggtgct
     9421 atgctgggcg ataactttct ggaattattc atcgttaaga tgggttgggt taagacactt
     9481 aacatgactg cagcttttgg tattgtattg actttagttt tgtgggttgg gatcagggac
     9541 aggaagggac agcacaggca aagcggtaca gttcccactc ttaagaaagg gttagttgat
     9601 ctgggaatta ttattaccag ccgacagata tgggttaatg gaatgtatgg ctgtttagtt
     9661 tatctgccaa ctacggtttt tgcagagttg tggggtattc cctatttaca ccatgcccat
     9721 ggtttaacta cccaagcggc aggattagct aactctgtac tatttttggg atttattatt
     9781 ggtgctccaa taatgggata tatttcggat cgtttgtatc gacgtaaatt cccaatgcta
     9841 attggtgcta gcggcgctgc aattgtcatg atgatgattt tatatttgcc tggattaaat
     9901 gaatccaata ttcaagcttt aatgttttta ttaggattgc tttatagcgc tcaagctata
     9961 gtttttgctg tgggcagaga gctgagccct ggtgaggcag caggaacagc aatggcggta
    10021 actaatatga tagttatgct cggcgcaatg tttttgcagc cgttggtggg gcatttatta
    10081 gacttcagct tatcaactca tttaagtgat actactggta ttgttgtgga taacatgagt
    10141 aaactatata ccgtagatga ttatcagttt gccttatcca ttattccatt aggtatattg
    10201 atagcggcca ttttaacttt ctttctaaaa gaaactcatg caaaagcacc taaataatat
    10261 gactactaaa aaacaaattt catatggcgt cctggtttgc tttatcgggg ctgtttttta
    10321 ctgttatgaa tttattctca gaattgttcc gggagcttta caaacggagc taagtacagc
    10381 tttgggacat atttctgcta ccacctttgg acaaatttca gccctgtatt attttgcata
    10441 ttcacccatg caaatgcctg tagggatgtt aatggatcgc tatgggccga gacgactttt
    10501 gacttttgcc tgtttgtgtt gtgccttggg ctcgtggatg tttactttga cctggtcaat
    10561 tgtgctagtt ggaattggtc gattcctggt gggatttggc tcatccttcg cttttgttgg
    10621 tgtcttgtct ctggctttgc attggctgcc acgtcgttat ttttctttag tggctggttt
    10681 gattactacg ctaggtatgt taggactggt ttatggtgaa atcaaaataa ctgaatggtc
    10741 agaggtcata ggttgggatc atgttttaat gcacattgca atgattggtt ctgcgctgtg
    10801 tatcttaact ttctttgttg ttagagatgg tccagaagga tatcaaccca acaaatatcc
    10861 ttggcccgag ttttttcata acgtgcttaa ggtactgacg tcccatgaag tgtggcttat
    10921 tgggtttgtt ggagcctgcc tttatacttc attatcggta tttggagagc tgtggggtaa
    10981 aacctatttg gagcaagcac atcatttaac caaagtagaa gccgcaaaaa ctgtttctgc
    11041 tgtttttctg gggtgggcgg taggagcccc cttggctggg tacatatccg atattacccg
    11101 cagacgattg ttgcctttgg tgattggtgc gatagtttca ttaatatgca ttagttttgt
    11161 tttgtattgc cctggcctat cctatcttac cttgaacata ttgctattct tgtatggtgt
    11221 tttcagtgca actgaaatta tagtttttat tatggctaag gaatgtagtg gagccaaatt
    11281 atcaggaacg gtttttgcag taaccaatat gattatttct ttggctggtg ctatttttca
    11341 accgctcgta gggaagttac tggatacatt tggcgacagc ggtatagtgg caggggaaca
    11401 tatctatacg gttgatgatt atcaagtggc tctttcgata ttacctattt cattactttt
    11461 ggtaactatt ctggctttct ttattaaaga caaagaccat tccaataact cttaatcttg
    11521 tgataaagac tcttctttgt ctttgtcagg atggacagtt gaatactgcg actagctctt
    11581 taaccggcag tcgcatctga cttctgcagc ttatccagcg aggtacatag aaactcttta
    11641 tttgtgcttt tttgtaatga tgtcgcgtaa attcagtatc atgattcgat ataatgacag
    11701 gaatcccttt ggaggctgtt tcctgggcca attcagccaa ctcaatttga tcttctaaag
    11761 aaaataattt ttggttatag ggtaatgttt gattgttttc ggtcagtggc acatagggtg
    11821 ggtcacagta aatgacatca ccgggttcag ctaaggcaaa agtgtttcta aaatcgccgt
    11881 gaatgaactg tgccgaaagg cattttttat aaaacagcat catttcctta cgtggaaaat
    11941 aaggtttttc gtaaagtcca aaagggacat tatattttcc ttttgaattg tatcggcaca
    12001 aaccattata tccatgccga ttaagataga gaaaaaccgc tgatttttga tttgaatcca
    12061 ttaaattatt aaattcactt cgcaattcat aatatttttc tttgcaattg gcttcggggg
    12121 taaagtattg ctggcaatag tctataaagg attcaccatt taattgtaga attctataaa
    12181 tattaaccaa atcagaattg ctttcagcca gtaggtaaga tgaatagctg gtgttcataa
    12241 acaccacgcc tgaacctgca aatggctcta ttaaacgctg gccagtgggt aaagaagaga
    12301 taatttcatt taaacaattg tacttacttc ctgcccattt cagaaaaggt ctgattttta
    12361 ccataactta taaaatcaat attttaggat gaagtatctc ttattttcag gataaagaac
    12421 aggataatga ataaaggttc cttatctgat ttacaaaata gcccttgtac tggtttcgct
    12481 ttactttaaa atatatcaaa tttgtacttt ttaacgctga tattcgggct ttaattaata
    12541 catgatcgca atgataagca aaccaatggc gataaatcct ataaggctta aagctggatt
    12601 tcctccagga atcaaatgag tttctgatgg agattggcta caggtttgtc ttcctttcca
    12661 tgccatgata gaaggtaata acagcagtaa aatgacacaa caaaccccgg cataacttaa
    12721 tgcatgcagg tagatacccg ggttaaacaa caccacggcc agtggaggga taaaagtgag
    12781 agccagcgta tacttgcctt gattaccaga ttttttaagc tttaatccat cagcaagaaa
    12841 atcaaataat cctaatgaaa ctcccagaaa tgcagtaacc atacagatag aagtgaaaaa
    12901 cccaaaaaat ccactgatcc actggctgct taccgattga ctaagtgctt cagtgaggcc
    12961 gctggttgca tgatccgagt tcattaggga caataaacca ttttctccct cgcgtgagat
    13021 aacgcccata atcactgcat cccagactat gtagcagaat aatgggataa gagaaccaaa
    13081 caaaataact cgacgtaagc tacgtatatc atcattaaaa taatcacgta agctgggcac
    13141 gatggatgca aatccaaaag aggtgaccaa gatcatgaga gtgccagtaa aggctttcgc
    13201 tgagccgcct gttaatcctg ctgtggatac atggggactg ataataataa ccaataaaat
    13261 ataaacgccc agtttgccaa acataagtcc acgattaacg tagtctacag agcgtatacc
    13321 actatataca ataaggctga acatggcagt aaatattaca gacgttatcc aattcggtaa
    13381 atcaatgtgg gccttatgca gcaggctgct aaatacatca cttccacctg aaatataagc
    13441 cgccaggagg gtataaagaa gaagaaggta ggttatccag gcaattacct gacctggcag
    13501 gcccaaggtt gatttcgcca ttgaaatcat gttgcttcct gccgggagtc gtagatttac
    13561 ctcaagaatt aataaagctc cagcggtcat caccaaccag caaaaaaaca ggaaaaataa
    13621 tgagttagta aatcccacct cggctgtaga aacaggtagc gctagcatcc caccaccaat
    13681 cgaagtgcct actatgagta atataccacc aattaatttt gaattgatca cgaagtataa
    13741 cccaaaaaac agtacaaata cagaacaaaa tgatactttg ttcctcaaaa aaagtcaact
    13801 tccttattgt agacctcaat cattttcttt tcgcaaatga cctggcgttc aatttataca
    13861 tcctgtatat caggttttaa tgttttattg ctataaagta aaaacaaggc cagtaataac
    13921 attaatcatt tttatttaaa aagcgcaata aattcatgtg ccaataagac tacagaaata
    13981 ataatcactg caaactgtga tattttacct cctggaaccc aaaagggtga tttgtaacgc
    14041 tttctgccaa aataacacat tagggctggc aataatagta gtaatatgat gcaaaatata
    14101 ccggcgtagc gcagagcata aatataagcc cctggataat aaatgacgat tagtaaaggc
    14161 ggcaaaaaag tcaaaacaaa taagccaatt ccatggctgc ctcgctctcg caattttaag
    14221 ccatcagcaa gaaaactaaa taaacaaagt gatacaccta agaaggcagt taacatgcaa
    14281 atggaggtaa aaaaattaaa gagggcgctg attgtggtat tttcaacttt atcagttaac
    14341 aaactggcta acgcgctggt tgtacgcgga ttttgcatta gactttcaag tccgctagtg
    14401 cctgctgaag ggagtgaacc aataattacg gcatcccagg ctatgtaaca tatcaaaggt
    14461 attaatgaac caataaaaat gacttttttt aatgtcttga tgtcatcatc aaaataatct
    14521 ctcagatttg gcacgataat agcaaaacca aaagaagtaa ttaaaatcat aattgcgcca
    14581 ccaatttgtc gataatcacc atgttgaaaa tagtgaaatt cgatgtgtgg ggcaatcagg
    14641 ataacaagta tgcagtaaac ggctaattta ccgaacatga gagcgcggtt agcaacatcg
    14701 acggaatgga tgccaccata taccaccagg ccaaaaatta aagtaaataa cgaacttgcc
    14761 tgccaatcac tgagatgcca attgactttg gacaatagac tgttcaatac atcagcgcca
    14821 ccggaaatat aagcggaaag caaggtatac aataagaaaa ggtaactaat ccaggcagta
    14881 agtaacccat aatcgcccag agttgccttg gccatggaaa ccatgtgttt tcctctggac
    14941 aaatagagat ttgcttccag gatgaaaaag gcaccaaagg tcataaaagc ccagcagata
    15001 agaagaaaca gagaagattg ccaaaaaccg gttgctgcat tagctactgg tagagccaac
    15061 atccctccac caatggaggt accaactatc aataagatgc cgcctataaa tcttgaacgc
    15121 attaataaac ctttatttta cataagccat ttgtggttgt ggaggttgag ccgggcttgt
    15181 aaaccactgg atctcgattc ttctgttctg agcgcttcca tgaataatag cattgtcaga
    15241 aatcgcattc ttatctccat agccttctgc ttttagtctt ttggctgcaa taccattagc
    15301 ccagagaaaa gtcatcattg tttcagcttg cgcttgagaa agttttcttt tatggctgcg
    15361 tgatccgaca ttatcagtaa atccggcaac ataaatcgta ctttgaggat aaaaatttaa
    15421 cagcctgatg acattgttta ggccaggata gcatatttca ttaaggcgag ggctgctaaa
    15481 catgaaatat ttgtctgtag gaataattaa tgttcttgta tctccatatt caacatactg
    15541 aatatcctgc ttttgcagat ctctgatgat tttgcgtttg ctgtcacggt agatactcgc
    15601 aactagacct acggtaccac cagccgctgc cccaataaga gttcctgaag cagtaccact
    15661 ggctattacc ccagctacag caccaatccc tgtatctact cctactctct taacagctcg
    15721 tctatcgggt tgaaagttat tataaggggg atgaaagcag cccgcaagaa agcaaatcat
    15781 taatccataa ccaaggattc gtgttttggc taatttgcgt gacatatcca ctcctttaat
    15841 caatgattta atcattatta agcatctcat taaatttcgc aatctaacac tttatatttt
    15901 tacaggacgt ataaaaaaat aattttcttc gtctataatt gaaatagtca aggaagaaac
    15961 agagtgatgc actgtgaaag gaaattcagt gtgcccactc gcccaaatgc aaggagcggt
    16021 atgcctggct cattacccgg tttaccgaaa aagctatgaa aattccagtc caactcaaaa
    16081 atataggcgg taatatccca gaaaatgata ttgctttgca agtttcttat tttataaatc
    16141 tcaaaataac gaattgcttc cccaaagtga cgcttatatt gctccagctt caggcaagat
    16201 tgatgtcagt tttggaaata atctcgcgcg aaataaagat gttttctttg ctgtaatcag
    16261 tactgcagac aagaaaataa ttttgctgaa tcttaaggat gataattggg tttgatatgg
    16321 gca
//
LOCUS       DACTJE010000062        15928 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.062, whole genome shotgun sequence.
ACCESSION   DACTJE010000062 DACTJE010000000
VERSION     DACTJE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15928
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.062"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(221..1063)
                     /locus_tag="I9760_001539"
     CDS             complement(221..1063)
                     /locus_tag="I9760_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAT5043311.1"
                     /translation="MHHLILGYGYCGYYLAQELLGHHQQVTVVSRQLKKELELSKLQH
                     ISHDLEYPFHWSEPDSIIYYLIPPPSQGTCDTFLQQFLNQSSLNAKKIIYFGSSGVYG
                     NHQGAWVDELSACIIDSPRQQRRLDAEQQWLAYCQQHNKELSLLRIAGIYGPNRIPAD
                     AAKSKIPIIKPTEAPFTNHIFVKDLALIAYLLGQKQTTSAVYNVADGNPTHMGELQQT
                     VANALGIESASYESFEQAWEKASPMKREFMRASKRLKIDLLKTALGEDIQFTSLYDAV
                     KQSL"
     gene            complement(1091..3667)
                     /locus_tag="I9760_001540"
     CDS             complement(1091..3667)
                     /locus_tag="I9760_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HAT5043312.1"
                     /translation="MHWQKVNQKDMPIKFTDELKSDELYNLLSQGAVVITPNNRLSGT
                     ILQNYFTYWSRKTITKPICLPFNTFLVNSFEQLKFQKPDLEHPILLNSAQCQHLWRKL
                     IESHPSIIYTEGLLKTIMQAWEYCEQWQLTPEAPAFHYTPQTQQFQQWWQSFNKILKE
                     ISAIHQHQLIPYLLDSNYQGLLKTVVWMCFDDFNPQQLSLQNYLNAQGVIQYGYDLKE
                     NQETPEVFSAKDKKTEYQELMTWLEKKIQQGKKQIGVVVPNLEQESSVLQRILRDCFE
                     PNLFNISLGKPLSAFHLISHALIWLSLDHNLLNNHQAALLLQSPYLGKAKDEFLQRSD
                     YLQESKLLQDQSFPTKLFIEDIAHHTPKLAELLKNMNFYPEKTTLDEWIELFQERLNG
                     IGFPGDYVLNSEDYQCFNRFTMIFDEFRQLSLISSHLTKKEAFEALKQLIDNTIFQPQ
                     KSNSPIQILGLLEASGCEFDSLWVMGLTDRCLPQQTRLSPFIPFELQRELGMPHSIPA
                     RELQYAKQILQRLQRGSVETVFSYPQFDDDTPNLPCSLITHYPAYLSRPADNKTNVQS
                     CLVPLEENYEVPVKPDESISGGTSLLSNQAKCPFKALAQHRLKANPVLKTTDGMDDKE
                     KGQLIHKILELLWQRLENQEHLIKLKPAELERFIDQAIQGALNPIRQQHSQTFPDLVA
                     NVEYMRLKRIILSYLEWEKQRPPFKITGLEENHSINLAGLDFKVRVDRLDEVGNKQWV
                     IDYKSTLPGGKPWNEERPMEPQLLLYALLNEQINTLLLIQLKTGKIKLSGFSEEKWET
                     KEINTLKEDENWEDYRQRWLEQLTHLAEEIKSGYCPPRPQNLTICQRCDFQNLCRFQA
                     KQ"
     gene            complement(3615..4175)
                     /locus_tag="I9760_001541"
     CDS             complement(3615..4175)
                     /locus_tag="I9760_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043313.1"
                     /translation="MNTPDPALSISNPRLLSAIYFGLLSVVGTILINAFLTSIGIEEI
                     IPVYQSVILGMVVASCTGAIFGEAIVRCKKPYKRKTFWLGFSMVIASLPVFDLGLVFL
                     MSEDHAKLFEVTTLTSMVLFYLIVLAYSYVLFGILLAIASGLAAMYLRGQLVYDILHT
                     QTRRQQKHKHALAKSKSEGHAHKVHR"
     gene            4524..5762
                     /locus_tag="I9760_001542"
     CDS             4524..5762
                     /locus_tag="I9760_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAT5043314.1"
                     /translation="MNSILFKNANVILGESTELKKKFDVLVQNNLISQVSQTPLQPFE
                     GMRIIDVKGKTLMPGLIDAHAHITGLTLSPKNIFYSEAEIFLAAATYLKNSLFYGFTT
                     LREAGGADFRIAQLLDNKSIPGPRLFYSGRALTQTGGGADFRKPNEQIDPCGHVGSFS
                     TMSVIADGIDEVRKAAREELRKGATQLKVFASGGVVFPSLSNPTLYEYSEEELSTIVE
                     EARARNTYVMAHAYSDESVRKCIKSGVRSIEHANFVSEPTVELMSESGVFYDPTFISL
                     VQRIESAEQNRLSEAIVANLKNTIEKGKKVYEYALKYKIPIAFGTDLWGPEAQRDQLR
                     EFEMLKELDSAANIIRSATVVNAELLMQKGKLGVISEGAYADLLVVDGNPLVNLNVLL
                     RPDENLKLIMKDGVIYKNEL"
     gene            complement(5795..6346)
                     /locus_tag="I9760_001543"
     CDS             complement(5795..6346)
                     /locus_tag="I9760_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HAT5043315.1"
                     /translation="MLYHDPLISEHTMKWVRTFIINYTICPFAKGPVNKGTLRIITSD
                     TPKKHQALKDLITEIQFLEANPAVETTLLIFARAFKDFFSYLDFISLAEQLIQNLNYE
                     GIYQLATFHPDYYFADTSPDDVANYTNRSPYPMIHLLREDSLDKAIAAYGDTHTIPEK
                     NIATMNELGLEKMKQLLASISIP"
     gene            6464..7027
                     /locus_tag="I9760_001544"
     CDS             6464..7027
                     /locus_tag="I9760_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAT5043316.1"
                     /translation="MQKICRFIFKLIGWKIVGELPNEKKYIVIVAPHTSNWDFVICLC
                     ARFVVGVKINFLAKHQLFFFPLSYFLRAVGGTPVDRSKKGNRVEKVVELFRQHDELKL
                     GLAPEGTRSPVTRWKEGFYHIARQAGIPIVMVGPDYSTKEIRIHEPFWTTGDIKKDFQ
                     CIIEFFRTIKGYRPKEIPDYNPKDSDA"
     gene            complement(7024..7338)
                     /locus_tag="I9760_001545"
     CDS             complement(7024..7338)
                     /locus_tag="I9760_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAT5043317.1"
                     /translation="MGKDNLLIGMLIEETTTISFIEVCQKYHIPEELFIEMIEQGLFP
                     DQPSDPEKIALGQKELRRLESAFRLHRDLGINLPGVALALDLLEKIDQMHHELEILRK
                     HF"
     gene            complement(7341..8231)
                     /locus_tag="I9760_001546"
     CDS             complement(7341..8231)
                     /locus_tag="I9760_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HAT5043318.1"
                     /translation="MDKDYYKIMGVSQDASEKDIKMAYRKLARKYHPDISKEPDAEER
                     FKEMAEAYEVLKDPKKRSEYDQYLKYKEFNPQSDGFTGRRTQEQPFQEFHFDSDFFET
                     LFGHSRYQQQPMTGQNYHGKITISLEEAYHGAVKNLQVPVEQGTETKIQTLKVKIPAG
                     VKSGQQIRLAGQGGSGSGGGARGDLYLTVEVMKHPIFDVMENDIYLTLPVTPWEAALG
                     ATVVIPTLAGKIDLKIPPGSQGGQKLRIKNRGLPGSPPGNQYVLLKIITPPAQTEEAK
                     ALYKKMAEVMPYNPRKTMGV"
     gene            complement(8271..9389)
                     /locus_tag="I9760_001547"
     CDS             complement(8271..9389)
                     /locus_tag="I9760_001547"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HAT5043319.1"
                     /translation="MRHSALLIINSGSSSVKFQVFSNTETLDLLAKGKIINIGGEPSF
                     TAVNETLEHQRTLITLEKDMTYEQAIHLILDWISQQQQSWEIHAVAHRVVHGGEYFTQ
                     SVLVTPQIISQLETLNSLAPLHQPHNLAAIKIIGRIKPELPQIACFDTAFHAQHTPLF
                     TTCALPEYLRQQGIRRYGFHGLSYEWISLVLKKDYPDLANNRIIIAHLGNGASLCAIN
                     RGISIDTTMSLTALDGLPMGTRCGSLDPGAVIYMMRELNLPPDKVEEILYNESGLLGL
                     SGKTNNVLELQSSPDKNAQFALEYFCLKTAQFIGMMAISLGGVDGIVFTGGIGENSEP
                     IRANILKRIECLHPFSVIVIPANEEKMMAMHTLTALRT"
     gene            complement(9386..10792)
                     /locus_tag="I9760_001548"
     CDS             complement(9386..10792)
                     /locus_tag="I9760_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HAT5043320.1"
                     /translation="MNNEFLENVTYEELTLGRRASLNRTLTQDDINLFAAMSGDINPS
                     HVDPQFAKSDVFHGVVGHGMWSGALISTLLGTVLPGPGTIYLEQDIQFITPVRIGDNL
                     TITIVVRDKHPGKSIVHFDCMGVNQKGETVIKGLAKVIAPTKKMKVPRTVLPLVQIHH
                     NDHFNKIIEACSNLPSIKTAVVHPVTANVLEAVYDSVKAGLITPVLIGPMAKIKCAAS
                     EAKIDLSNWEMIDTEHSDAAAFKAVELAASGKVDSIMKGALHTDELMSAVVPATSGLR
                     TKYRISHAYVMDVPTYHKPLIITDAAINIAPNAADKADICQNAINLWRILYGEEIKPK
                     VAILAAVEIVNPKMQATVDAAVLCKMADRGQIINGILDGPLAFDNAINKQASKEKNII
                     SPVAGDADILLVPDIESGNMLAKQLTFLGHADAAGIVLGARVPIILASRADSLRTRLL
                     SCAVATKIAAARKAGKIK"
     gene            complement(10804..12591)
                     /locus_tag="I9760_001549"
     CDS             complement(10804..12591)
                     /locus_tag="I9760_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAT5043321.1"
                     /translation="MKKTNEKPINKTNEMPASSKKEIVSPLEQTPASEQSDPIFRFID
                     KLYQANLGKLTAGISPAALGTAYYSWFAQLLQSPGSMLRLASYPLLHANDYLSNLFKY
                     DKPRDGKDVRFHTDNWSYYPWRLWAEQFLQFEDWCLQASSKVPGIPLHVKRTVTFSTR
                     QILDALSPSNFVLTNPDLLQETIRSNGQNLIRGTELAFQDFVEKITGSPPAGVENFIP
                     GKQVAITKGKVVYSNHLIELIQYTPQTEKVYKEPILILPAWIMKYYILDLLPENSLVN
                     WLVRQGHTVFIVSWRNPTKEDRNLGLDDYYKLGAMDAINAVSNAIPHTKIHLMGYCLG
                     GTLALLTAAAMAHDHDNRLKTLSLLAAQGDFIDAGELLLFITKSEVSFLKSMMWEQGY
                     LDTKQMSGTFQMLRSYDLIWSKMVQDYMHGTQRGMIPLLAWNADATRMPYKMHSEYLE
                     KLFLNNDFAEGRFILDGKPVVGENIRIPAFVVSTEKDHVAPWKSVYKTHLLINSDITF
                     VLTNGGHNAGIVSEPGHEGRYYRIRERKMDSTYLDPTTWVKRAELREGSWWIAWHDWL
                     VNHSSQKQVSAPKLDKKLPNAPGKYVLQK"
     gene            12808..13119
                     /locus_tag="I9760_001550"
     CDS             12808..13119
                     /locus_tag="I9760_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HAT5043322.1"
                     /translation="MLKCLSSVIIVFLILFTTGCQTNTVSNMFIPSNPSGMTLAQSVK
                     DSLMESNDPVINQIHVESNQNVVILSGYVKKIRQSDIAEQIARQVQGVQSVENRIIVR
                     P"
     gene            complement(13210..13500)
                     /locus_tag="I9760_001551"
     CDS             complement(13210..13500)
                     /locus_tag="I9760_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043323.1"
                     /translation="MNGCKISPVGLGLSLGILWGLSLLVMGLIAYFYAYGRPFVEAVA
                     TLYLGYEPSILGSFIGGVIGFIDGFVTGFLIAWLYNCFSCCSCCRGKENKKE"
     gene            13617..14267
                     /locus_tag="I9760_001552"
     CDS             13617..14267
                     /locus_tag="I9760_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043324.1"
                     /translation="MNYFIGLFFGLLNAVICYAEPNGSSLPKELIKVNKENNPKCVEF
                     VTYKGELYCSVVPLADSSGDSQVIHYEKQIVRFDDRPWKIAWGKKTDKVATVEYIPVG
                     DDINNWKELITTQFMPGLTHMTPAQFGNQFLYNLNQSGVKYIVHVIEEKPELLIFEFK
                     IQKPANLQQDEILKITKGKDGIYVLHYAIKKSDMTKENRDKWIKNLKNSFIKISTP"
     gene            14390..15748
                     /locus_tag="I9760_001553"
     CDS             14390..15748
                     /locus_tag="I9760_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT5043325.1"
                     /translation="MKLSFLGATGTVTGSKYLVESNNKKILVDCGLFQGLKELRLRNW
                     SPLPVSPKSIDAVVLTHAHIDHSGYLPLLVKQGFKGRIYATAATTDLCSILLPDSGFL
                     HEEDARRANKYGYSKHHPALPLYTQEDANNTLKYFESVEYDTPYPLMDDCEISWHRAG
                     HILGSAFIQFQTKQIKLLFSGDIGRLNDPVMKPSELMKSTDYLVIESTYGNRLHDKTD
                     PLAFIANIVNTTCKRGGTVLIPAFAVGRAQTMLYYLYILKQKKLIPDVPVYLDSPMAI
                     DATELLLKHHNEHRLSAEECRAVCQIATYVHAPNESMAVDWERESKIIISASGMMTGG
                     RVLHHLKVYAPDPASTILLTGFQAAGTRGEKMLNHEPSVKIHGDDYPVRAQVATLTNA
                     SAHADYEEILIWLKHFEKAPKEVFITHGEPQAALGLKGHIETELGWNCKIPDYLQKVN
                     LD"
BASE COUNT         4644 a         2921 c         3341 g         5022 t
ORIGIN      
        1 ttgatttaat tgtgtatcac agttttgcaa acgtttttcc aagagccggg cattgttgta
       61 agaaggcata gtttacctcg attgtaatta taaatttaaa atcctattga tttataacga
      121 attattggat tatatataac caatgtggca gcataatacc atattttaaa gaaaattaaa
      181 gtttaactta tcaagaatag ttttttacaa aaacagaaaa tcataagctc tgttttactg
      241 catcatacaa actagtaaat tgaatatctt cacccaatgc agtttttaac agatcaatct
      301 tcaatcgttt ggacgctcgc atgaactctc ttttcatagg gcttgctttt tcccaagctt
      361 gctcaaagga ctcataggat gctgattcaa ttcctaatgc gtttgctacc gtttgttgta
      421 gttcgcccat atgggttggg ttgccatcgg caacattata gacagccgat gttgtttgct
      481 tttggccaag caaataagct attaaagcca aatccttgac aaaaatatga ttggtaaaag
      541 gggcttcagt cggttttatt attggaattt tcgatttggc tgcatcggct gggattctgt
      601 tcggtccgta tatgcctgca atacgcaata aggaaagctc tttattgtgt tgctgacaat
      661 aggccagcca ttgttgttct gcatccaatc gtctttgttg acgtggggag tcaataatac
      721 aagctgataa ttcgtcaacc caggctcctt gatggtttcc atagactcca cttgaaccaa
      781 agtaaataat tttctttgca tttaatgaac tttgatttaa aaactgttgt aagaaagtat
      841 cgcaggtacc ttgagaaggt gggggaatta gatagtagat aatggagtcg ggctcgctcc
      901 aatgaaatgg gtattcgaga tcatggctta tatgctgtag ttttgaaagt tccaattcct
      961 tttttaattg tcgtgatact acggttacct gttgatgatg cccaagaagc tcttgggcca
     1021 ggtaataacc gcaatagcca taaccaagaa ttaaatgatg cataagtagt cccgataaat
     1081 taaaactggt ttattgttta gcctggaagc ggcataagtt ctgaaaatca cagcgttggc
     1141 aaatggtcag attttgaggg cgtggtggac aatagccaga cttaatttct tcagctaaat
     1201 gagttaattg ttctagccag cgttgtcgat agtcttccca attttcatcc tctttcaaag
     1261 tattaatctc tttggtttcc catttttctt cgctaaaacc gctgagcttg atttttccgg
     1321 tttttaattg aatcaataac aaagtattga tttgttcgtt aagcaacgca taaagtagca
     1381 attgaggctc cattggtctt tcttcattcc atggtttgcc gccagggaga gtgcttttgt
     1441 aatcaatgac ccattgcttg ttacctactt catccagtct gtccactctt actttaaaat
     1501 cgagcccagc caaattgatc gaatgatttt cttccaggcc tgtgatttta aaaggagggc
     1561 gttgcttttc ccattccaga taagacaaga taattcgttt taatctcatg tattcaacat
     1621 tggcaaccaa gtcaggaaaa gtctgggagt gttgttgcct aatcggattt aaagcgcctt
     1681 gaattgcttg atcaatgaat cgttcaagtt cagcgggttt taatttaatt aagtgttcct
     1741 gattttccaa tctttgccag agcaactcca ggattttatg gattaattga cctttttcct
     1801 tgtcatccat tccatctgta gtttttagaa cagggtttgc cttaagtcgg tgttgtgcta
     1861 atgctttaaa agggcatttg gcctgattgc ttagtaagct ggttccgcca gaaatggatt
     1921 catcaggttt tacaggcact tcatagtttt cttcaagagg aaccaaacaa ctttgaacat
     1981 ttgttttatt gtcagcaggc cggctgaggt aggcaggata atgagtaatc aatgagcagg
     2041 gtaaattagg cgtgtcatcg tcaaattgtg gataactaaa aacggtttcc acgctacctc
     2101 gttgcagtct ttgcaaaatt tgcttggcat attggagctc tcttgccggg atactgtgag
     2161 gcatacccag ttcacgctgt aattcaaatg ggataaaggg cgataaacgt gtttgttgag
     2221 gtaaacatcg atcagtcaat cccattaccc aaaggctgtc aaactcgcaa cctgatgctt
     2281 ctaacaatcc caaaatttga attggtgaat tgcttttttg tggttgaaaa atagtgttgt
     2341 ctatgagttg ttttaacgct tcaaatgcct cttttttagt caaatggctg cttatcagac
     2401 ttaattgtct gaattcgtca aaaatcattg taaagcgatt aaagcattga taatcctctg
     2461 agttcaaaac ataatctcca ggaaaaccaa tgccatttaa cctctcctga aataactcaa
     2521 tccactcatc cagggtagtt ttttccggat aaaaattcat gttttttaac aattcagcca
     2581 gtttaggcgt gtggtgggca atgtcctcaa taaataattt tgtggggaaa gattggtctt
     2641 gcaacaactt gctttcctgt aagtaatctg atctttgaag aaactcatct tttgccttgc
     2701 caaggtaggg tgattggagc agcaaagctg cttgatgatt atttaagaga ttgtgatcaa
     2761 gactcagcca aatgagggca tgagagatca agtgaaatgc gcttaatggt ttgcctaaag
     2821 aaatattaaa taaattgggt tcaaagcagt cgcgtaaaat tcgttgcaaa accgatgatt
     2881 cttgttcaag attaggcaca acaaccccga tttgcttttt tccttgttga attttcttct
     2941 ccaaccaggt catcaattcc tggtattctg tttttttgtc tttagctgag aaaacctccg
     3001 gagtctcctg gttttctttt aaatcataac catattgaat taccccctgc gcgttcaaat
     3061 aattttgcag ggataattgt tgtggattaa agtcatcaaa gcacatccag acaacggttt
     3121 ttaagagccc ttgataattg gaatcaagca agtagggaat taattgatgc tgatggatgg
     3181 cagaaatttc tttaagtatt ttattaaaag attgccacca ttgttgaaat tgttgtgttt
     3241 gcggcgtgta gtgaaaagca ggcgcttcag gtgtaagttg ccattgctca cagtattccc
     3301 atgcttgcat tattgtttta agcaagcctt ccgtgtaaat gatagaagga tgagattcaa
     3361 ttagttttcg ccataaatgt tggcattgtg ccgaatttaa aagtatggga tgttccaggt
     3421 ctggtttttg gaatttgagt tgttcaaaag agttaaccag aaatgtatta aaaggcaagc
     3481 atattggctt ggtaattgtt tttctggacc aataagtgaa gtaattctga aggatggttc
     3541 cgcttaatct attgttagga gtaataacga ctgctccttg agacaataga ttataaagct
     3601 catctgactt aagttcatcg gtgaacttta tgggcatgtc cttctgattt actttttgcc
     3661 aatgcatgct tatgtttttg ttgacgtcga gtttgggtat gcaggatatc gtatactaat
     3721 tgtcctctta aatacatagc ggcaagcccg gatgcaatag ccagcaaaat accaaaaagc
     3781 acataactat aggccagaac aattaggtag aacaaaacca tgctggttag agtggttacc
     3841 tcaaaaagct tggcatgatc ttctgacatc aagaagacaa gaccgagatc aaagacaggc
     3901 agtgaggcta tgaccatact gaatcctaac caaaaggttt ttcttttata tggtttttta
     3961 cagcgaacga tggcttcccc aaaaatggcc ccggtgcagg acgctacaac catgccaaga
     4021 atgaccgatt gatagaccgg gataatttct tcaattccaa tcgaagtgag aaaggcgttg
     4081 atcagtatcg tgccaacgac cgataacaag ccaaaataga ttgctgataa taaccgtgga
     4141 tttgagatcg aaagtgctgg atctggtgtg ttcatgatgc acctgataat gagttctata
     4201 tgataaattt tagtacataa tttcaataat gtggacttgg gtttgattaa ttttctatag
     4261 ttatgggcaa gagtggtcgt tatctagatc tagaagactt gtatggaatt taatgtttcg
     4321 tgctaaggct tcgagacgat gcttatgcat ctcctcagcc caaacgaatc ctggatatca
     4381 attggattta tatcatcctt atcagtaact cacgttaaat taaatacttt tgaaaaagca
     4441 gggtaaagga aaatgagagc gagtcggagt tgccaaaata gactataaat agagtaagtt
     4501 agattaatga cttcgagatt aaaatgaact caattctttt taaaaatgcc aacgttattt
     4561 tgggagagtc tacagagctt aaaaaaaaat ttgatgttct ggtgcaaaat aatttgatct
     4621 cccaggtgtc gcaaacaccg cttcaacctt tcgaaggaat gcgcattatc gatgtcaaag
     4681 ggaaaacatt aatgcctggt ttaatcgatg cccatgctca tattaccgga ttgaccctaa
     4741 gcccaaaaaa tatcttctac tctgaagcag agatattttt ggctgctgct acttatctta
     4801 aaaacagctt gttttatggt tttacgactt tacgagaagc tggaggcgct gattttagaa
     4861 tagcacaatt actagataat aagagtattc cagggcctcg attattttat tctgggaggg
     4921 cattaactca aacgggtgga ggagctgact ttagaaaacc gaatgaacag attgatcctt
     4981 gtgggcatgt tggctctttc tcgacgatgt cggttattgc agatgggatt gatgaagtga
     5041 gaaaggctgc tcgagaagag ttaaggaaag gagcaacaca attaaaagta tttgcttcag
     5101 gcggggttgt tttcccttcc cttagcaatc caacactgta tgaatattcc gaggaggagt
     5161 tatcgactat tgttgaagag gcacgagcaa gaaatactta tgttatggcc catgcttata
     5221 gcgatgaatc agtaagaaag tgcattaaat cgggtgtgcg ttccattgaa cacgctaatt
     5281 ttgtaagcga accaactgta gaattaatgt cggaaagtgg tgtgttttac gatccgacat
     5341 ttatttcatt ggtgcaaaga attgaaagtg ctgaacaaaa ccggttgtct gaagctattg
     5401 tagccaattt gaaaaatact attgaaaaag gcaaaaaggt ttatgaatat gctttaaaat
     5461 ataagattcc tattgctttt ggtacagatt tgtggggtcc tgaggcacag agagatcagt
     5521 taagagaatt tgaaatgctc aaggagcttg attcagcagc caatattatt cgttcagcta
     5581 ccgtagtcaa tgcggaatta ttaatgcaaa agggaaaact tggcgtcata agcgaaggag
     5641 cttatgctga tctgttagta gtagatggta atcctttagt caatttaaat gttctgctcc
     5701 gcccggatga aaaccttaaa ttgattatga aagatggggt catttataag aatgaattat
     5761 aaagcaaggt ctttttcaga agtcctgttg aatactaggg tatagaaatg gaagccaata
     5821 actgtttcat tttttctaaa ccaagctcat tcatagttgc tatatttttt tctggtatag
     5881 tgtgggtatc accataggca gcgattgctt tgtctaggct gtcttccctt aataaatgga
     5941 tcatgggata aggggaacga ttagtgtagt tggcaacatc atctggagaa gtatcagcaa
     6001 aataataatc cgggtggaaa gtagcgagtt ggtatatccc ttcataattt aagttttgaa
     6061 ttaattgttc agcaaggctt atgaaatcca gataagaaaa aaagtcttta aatgctcgag
     6121 caaaaataag cagagtcgtt tcaacagcag ggtttgcttc cagaaattga atttcagtga
     6181 ttaaatcttt aagtgcctgg tgttttttgg gggtatctga agtaattatt cttaaagtgc
     6241 ctttgtttac ggggcctttt gcaaaagggc aaatcgtgta attgataatg aatgtccgca
     6301 cccatttcat ggtgtgttct gaaatcaatg gatcatgata gagcataggg taagtggttt
     6361 cgaaaaaagg gatattgtac cttgatcatg tgaaataaca atttcttcta aatagatatc
     6421 aaaatcaagt atactttggt taaaacaaaa atggattttt gtcatgcaaa aaatatgtcg
     6481 ttttattttt aaattaatcg gttggaagat agttggtgaa ttaccgaatg aaaaaaaata
     6541 tattgtgatt gttgctccgc ataccagcaa ttgggacttt gtcatttgcc tgtgtgcacg
     6601 ttttgttgtc ggggttaaaa tcaatttttt agcgaaacat cagttatttt ttttcccttt
     6661 gagctatttt ttgcgtgcag ttggaggtac tccggttgat cgttctaaaa aggggaacag
     6721 ggttgagaaa gttgtagagt tgtttcgcca acacgatgaa ttaaagctgg gcctagctcc
     6781 cgaaggtacc cgaagtccgg tgaccagatg gaaagagggg ttttatcata ttgctcggca
     6841 agcaggtatt cctatagtga tggtggggcc tgattattct actaaagaaa tccgtatcca
     6901 tgaacctttt tggaccacag gagatattaa aaaggatttt cagtgcatta tcgagttttt
     6961 tagaacgatt aaaggttatc gccctaaaga gatacctgat tataatccta aagattctga
     7021 tgcctaaaaa tgcttgcgta aaatttcaag ctcatgatgc atttgatcaa ttttttccag
     7081 taaatcaaga gctaaagcga ctccaggcag gttaatgcct aaatctctat gtaatcgaaa
     7141 ggcggattca aggcgacgca attctttttg gcctaatgca attttttcgg gatcagatgg
     7201 ttgatctgga aacaaacctt gttcgatcat ttcaatgaat aattcttcag gaatatgata
     7261 cttttggcat acttcaataa aagaaatcgt tgtggtttct tcgataagca tgcctattaa
     7321 aagattgtct ttgcccatgc ttatactccc atagtcttac gagggttgta aggcattacc
     7381 tccgccattt ttttatataa agcttttgct tcttcagttt gagcaggggg ggtaataatt
     7441 tttaatagaa catattgatt accagggggt gaacctggta atccgcgatt tttaattctg
     7501 agtttttgcc cgccttgtga gcccggaggg atttttaaat cgatttttcc tgccagggtt
     7561 ggtatgacaa ccgtagctcc taaagcagct tcccacggtg tgacaggtaa tgtcagataa
     7621 atatcattct ccatgacatc aaatatgggg tgtttcatca cttcgactgt tagataaagg
     7681 tcgccacgag ccccccctcc acttccactg cctccttgtc ctgccaagcg aatttgttgt
     7741 cctgatttaa cccctgcagg gattttaact ttcagggtct gtattttagt ctctgtgcct
     7801 tgttcaacag gaacttgtaa gtttttaaca gctccatggt aagcttcctc cagactgata
     7861 gtgattttgc catgatagtt ttgaccagtc atcggttgct gttgatagcg cgaatgtcca
     7921 aacaaggttt caaaaaaatc gctgtcaaaa tgaaattcct gaaagggttg ctcttgagtt
     7981 cttcgcccgg taaatccatc gctttgggga ttgaattcct tgtatttcag atattgatca
     8041 tattcggatc tttttttagg gtctttcagt acttcatagg cttctgccat ttctttaaat
     8101 cgttcttcag catctggttc tttactaata tcggggtgat atttgcgggc taattttcgg
     8161 taagccattt tgatgtcttt ttcagaagca tcctgactga ctcccatgat tttatagtag
     8221 tctttatcca ttttttatac tcattaccta tgccagtaaa caatacgcct ttacgtcctg
     8281 agggctgtca aggtatgcat agccatcatt ttttcttcat tcgcaggaat aacgataaca
     8341 gaaaaagggt gtaagcactc tatcctctta aggatatttg ctctaatcgg ttctgaattt
     8401 tcccctattc ctcctgtgaa tacaatacca tctactccac ctagtgaaat ggccatcatt
     8461 ccaataaatt gagctgtttt caggcaaaaa tactccaaag caaattgagc gtttttatca
     8521 gggctggatt gtaattccag cacattattg gtttttcctg ataaaccaag caagccggat
     8581 tcgttataaa gtatttcctc taccttatct ggtggaagat tcaattctct catcatgtaa
     8641 ataacagccc caggatccaa actgccacaa cgagttccca ttggtaaacc atccagcgct
     8701 gtcaaactca tggtggtgtc tatactgatt cccctattta tagcgcacaa gctagcgccg
     8761 ttgcctaaat gggcaataat aattcgattg ttagccaaat cagggtagtc ttttttgaga
     8821 acgagagaaa tccattcata ggaaaggcca tgaaatccat aacgcctaat cccttgttgt
     8881 cttaaatatt cgggcaaagc acaagtagta aataatggag tatgttgagc atgaaaggcg
     8941 gtatcaaagc atgcaatctg aggtaattct ggcttaatcc gaccaatgat ttttatggca
     9001 gccaaattat gaggctggtg taggggcgcg agagaattca gtgtttccaa ttggctgatg
     9061 atttgcggag taaccaggac actttgagtg aaatattctc cgccatgaac gacccgatgg
     9121 gcgacagcat gaatttccca gctttgctgt tgttggctaa tccagtccag tataagatga
     9181 atggcttgct catacgtcat gtccttttcc aaagtgatta gagttctctg gtgctccaat
     9241 gtttcgttaa ctgcggtaaa agatggttct cctccaatat taataatctt gcctttggcg
     9301 agaagatcaa gtgtctctgt attggagaac acttgaaatt taacgctgga ggaacctgaa
     9361 ttgatgatta acagtgcaga atgcctcatt ttattttgcc tgctttacga gccgctgcta
     9421 tcttagttgc taccgcacag gacaataaac gtgttcgtaa tgaatcagct ctgctggcta
     9481 gaatgatagg gactcttgct ccgagaacta ttcctgctgc atcagcatgt ccgagaaagg
     9541 tgagttgttt ggctagcata tttcccgatt caatgtctgg taccagcaaa atgtcggcat
     9601 caccagctac aggagagata atgttttttt ccttactggc ttgcttattg atggcattat
     9661 caaaggcgag ggggccatcc agtatgccat taattatctg acctcggtca gccattttac
     9721 acaggacagc ggcatcaacg gttgcctgca ttttgggatt tactatctca acagctgcaa
     9781 ggatagcgac cttgggtttt atttcttctc catataaaat acgccagaga ttgattgcat
     9841 tttgacatat atctgcctta tccgctgcgt ttggggctat attaatggct gcgtcggtaa
     9901 tgattaaagg tttatggtaa gttggaacgt ccataacata ggcgtgactg atgcggtatt
     9961 tggttcttag tccagaagtg gcaggtacca ccgcagacat taactcatca gtatgcaggg
    10021 cgcctttcat gatagaatcg actttcccgg atgccgctaa ttcaactgct ttaaaagcag
    10081 cagcatcact gtgttctgta tcaatcattt cccaattgga taaatcgatt ttagcctcac
    10141 ttgcagcaca tttgattttt gccataggac ctattaatac tggagtaatt aaaccggcct
    10201 tgactgagtc ataaactgct tccaggacat tggcagtgac gggatgaaca actgctgttt
    10261 tgattgaggg caagttactg caggcttcaa tgattttatt aaagtgatca ttgtgatgga
    10321 tttgcaccag aggcagaaca gttcggggta ccttcatttt tttagtcggg gcaatgactt
    10381 tggcaaggcc tttaattact gtttctcctt tttgatttac ccccatacag tcgaaatgaa
    10441 caatcgattt gccaggatgt ttgtcacgaa caacgatggt aattgtcaga ttatctccaa
    10501 tgcgaacggg agttataaat tgaatgtctt gttcaagata gatagttcca ggcccgggaa
    10561 ggacagtgcc cagcagggta gaaattaaag ctccagacca catgccatgt ccgactacgc
    10621 catgaaatac atcgctttta gcaaattgag gatcgacatg agagggattt atgtctccag
    10681 acatagcggc aaataaatta atatcatcct gagttaatgt acgatttaag ctggctctgc
    10741 gtcctaaagt cagctcttca taggtgacat tttcaagaaa ttcattattc attactaatt
    10801 cctttatttt tgtaatacat atttcccagg agcgtttggt agttttttgt ccagtttggg
    10861 tgcagacacc tgtttttggg atgagtgatt aactaaccaa tcgtgccagg caatccacca
    10921 ggatccctct cttaattcag ccctttttac ccaggtagtt gggtcaagat aggttgaatc
    10981 catttttctt tcccgaatgc ggtaataacg tccttcatgt ccgggttcac tgactattcc
    11041 agcgttatga cctccattag tcaggacaaa agtaatatcg gaattgatta acaaatgggt
    11101 tttgtatacg gatttccacg gagctacgtg atctttttct gtgcttacga caaaagcagg
    11161 tatacgaata ttttcaccta cgaccggctt tccgtccaga ataaatcggc cttcggcaaa
    11221 atcattatta aggaacagtt tttccaggta ttcactatgc attttgtatg gcatacgagt
    11281 tgcgtcggcg ttccatgcca gtaaagggat catccctctt tgagtcccgt gcatgtaatc
    11341 ctgaaccatt ttagaccaaa tcaaatcgta cgaacggagc atttgaaaag ttccagacat
    11401 ttgttttgtg tccagatacc cttgctccca catcatactt tttaaaaagg atacttcact
    11461 tttggttata aagagcagta attcgccggc atcaataaaa tcgccttgag ctgcgagcag
    11521 ggataatgtt ttaagacggt tatcgtgatc atgagccatc gctgctgcgg ttaacagagc
    11581 cagggtgccc ccaagacaat aacccattaa atggattttg gtatgtggaa tggcattgct
    11641 cactgcattt atggcatcca tagcgcccaa cttataataa tcatccagtc caagatttct
    11701 atcttctttc gttggattgc gccaggatac aataaataca gtatgtccct gtctaaccag
    11761 ccagtttact aaagagtttt ccggcagcaa atccaggata taatatttca tgatccaggc
    11821 gggtaaaatc aagatcggtt ctttgtatac cttttctgtt tggggtgtgt actggattaa
    11881 ttcaattaaa tgattgctat aaacgacttt cccctttgtt atagcgactt gtttccccgg
    11941 gataaaattc tcgactcccg caggaggaga accggttatc ttttctacaa aatcctgaaa
    12001 agcaagttct gttccacgaa ttaaattttg tccgttcgag cgaattgttt cctgaagcaa
    12061 atcaggattg gtcaaaacaa aattggatgg tgacaaggca tcaagaatct gtctggtgga
    12121 aaaggttact gtccttttga catgaagtgg tatccctgga actttgctcg aggcttgtaa
    12181 acaccaatct tcgaattgga gaaattgttc agcccaaagc cgccagggat agtaactcca
    12241 gttatctgta tgaaagcgta catctttgcc atctctgggc ttgtcatact tgaaaaggtt
    12301 ggaaagataa tcgttagcat gcaacagagg ataagaggcc agtcgtaaca ttgaaccagg
    12361 tgattgcagc agttgagcaa accaggagta ataggctgta cctaatgcgg caggacttat
    12421 accagcagtt aactttccca ggttagcttg atatagctta tcaatgaatc tgaaaatggg
    12481 gtcagattgt tcggaagcag gtgtttgttc tagtgggctt acgatttctt tttttgagga
    12541 tgcaggcatt tcatttgttt tattgatagg tttctcgttt gtttttttca taccttgcat
    12601 cctgtaggtg tttgatttat aaaaaatagt atagcagcat tccataaatg acaatttaat
    12661 ctgagttatt gtttgaaaag acttaagttt ctcttgctaa acgatgattt ataggcagtg
    12721 gcttgactat agaaataagg ggagttgtgc tattattcgg ttcttttaat ttagtgtaga
    12781 taaatccact ctatcttgga gattgtgatg ctaaaatgtt tatccagtgt aatcattgtg
    12841 tttttgattt tgtttacaac cggttgtcaa accaatactg tgagtaacat gtttattccg
    12901 tctaatccat caggcatgac tttggctcag tcggtaaaag attcgctaat ggaaagcaat
    12961 gatcctgtga tcaatcaaat tcatgtggaa tccaatcaaa atgtggtcat attaagtggt
    13021 tatgtgaaaa aaattcgcca gagtgatatc gcagaacaaa ttgccagaca agtccaggga
    13081 gttcagtcag tggagaaccg tattattgtt cgcccctaaa ctattttaac tcttgaataa
    13141 attaatttct cttgtcaatg acgttggttt taacgtcatt gaccacatga tttctcatta
    13201 ttagcaagct tattcttttt tattttcttt acctcggcag caagaacaac aactaaaaca
    13261 attgtaaagc caagcgataa ggaatcccgt gacgaaccca tcaataaagc caatgacgcc
    13321 accaatgaag cttcccagga tagaaggttc gtagccaagg tataaggtgg caactgcttc
    13381 aacaaagggc ctgccatagg cataaaaata ggcgattaat cccatgacta acaatgataa
    13441 tccccataga attcctaaag aaagacccag tccaacagga ctgattttgc aaccattcat
    13501 ggcaatctcc ttattagctc tctaatataa ttatatacca gtttgtctaa tcaaaattgt
    13561 gggtcatggg tttcaacgtc tacaattaaa tagtatccac ttttgggatt aagagcatga
    13621 attatttcat tgggttgttt tttggattac ttaatgccgt tatttgctac gcagagccta
    13681 atggctcttc tcttccaaaa gaattaatca aagtcaataa agaaaataat cctaaatgtg
    13741 tggagtttgt aacctataag ggagaacttt attgcagtgt tgttcctttg gctgattcct
    13801 caggtgattc acaagttatc cattatgaga agcaaatagt acgttttgat gaccggccct
    13861 ggaagatcgc ttggggaaag aaaacggata aggtagctac cgttgagtat attcctgttg
    13921 gagatgacat taataactgg aaagagttga ttaccacaca atttatgccg ggtttaactc
    13981 acatgacacc agcccagttt ggcaatcaat ttttatataa cctgaatcaa tcaggggtaa
    14041 aatatattgt ccatgtcatt gaggaaaaac ccgagctact catttttgaa tttaaaattc
    14101 aaaagcctgc taatctgcaa caagacgaaa tcttgaaaat taccaaaggt aaggacggaa
    14161 tttatgtact tcattatgca attaaaaaat cggatatgac taaagagaat cgagacaagt
    14221 ggataaaaaa cttaaaaaac agttttataa aaatatccac accgtaagtg caaaagaatg
    14281 ggcgggataa aaagttagca tttgaagctt caaatgactt taaaataaat tggcaatagg
    14341 tcgtattatt cattacagta ctgcgaatga catacaacaa ggtattatta tgaaattgtc
    14401 ttttttaggt gcgactggta cggttaccgg ctctaagtac ctggtagaat caaataataa
    14461 aaagattttg gtagattgcg gacttttcca gggtttaaaa gaattgagac taagaaactg
    14521 gtcacccctt cctgtgtctc ccaaatcaat agatgctgtt gttttgaccc acgcacatat
    14581 tgatcatagc ggttatttgc cattattggt gaaacaagga tttaaggggc gcatctatgc
    14641 aactgctgcg acaaccgatt tatgttctat cttactgccc gatagcggtt ttttgcacga
    14701 ggaagatgcc agaagggcaa ataaatatgg gtatagcaag caccatccgg ctttaccttt
    14761 gtatactcag gaagatgcaa ataatacctt aaagtatttt gaatcagtag aatatgacac
    14821 cccttatcct ttaatggatg attgcgaaat cagttggcat agggctgggc atatattagg
    14881 gtctgcattt attcaatttc aaacaaagca aatcaagtta ttatttagtg gtgatattgg
    14941 aagactcaat gatcctgtta tgaagccttc cgagttaatg aaatctaccg attatttggt
    15001 gattgaatca acttatggga atcgcttgca tgataaaacc gatcctttag cgtttatagc
    15061 caacatagtc aatacgactt gtaaaagagg cggtacagtg ctaattcctg cttttgcagt
    15121 cggtagagca caaactatgt tgtattattt gtatatttta aaacaaaaaa aattaattcc
    15181 tgatgtccct gtctatctgg atagccctat ggctattgat gccactgaat tattattaaa
    15241 acatcataac gaacaccgct taagtgcaga ggagtgccga gccgtctgcc aaatagcgac
    15301 ttatgtccat gccccaaatg aatccatggc agtggattgg gagcgcgaat ctaaaattat
    15361 tatttctgca agtggcatga tgacaggggg ccgggttttg catcatttga aggtatatgc
    15421 tcctgatcct gcaagtacta tccttttgac tggattccag gctgctggaa cgagaggtga
    15481 aaaaatgtta aatcatgagc cgtccgttaa aatccatggt gatgattacc ccgtcagggc
    15541 tcaggtagct acgttaacca atgcctctgc tcatgcagat tatgaggaaa tactaatttg
    15601 gctaaagcat tttgagaagg ccccaaagga agtctttatt acccatggcg agcctcaagc
    15661 ggcattggga ttaaaggggc atattgaaac ggaactgggg tggaattgta agatacctga
    15721 ctatcttcaa aaggtaaact tggattgata aatcccaaga atagtactta atttaatatt
    15781 ctgagcaaaa gtggttagtc ccctaaaaat cagctttagt tgaaacagtt gggcatgaat
    15841 tgaatctgat gatccagaat ctataaaatc tcagattgat tccattagac gctgagtctg
    15901 tttggtttaa tggctgggtt ttggtgat
//
LOCUS       DACTJE010000063        15735 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.063, whole genome shotgun sequence.
ACCESSION   DACTJE010000063 DACTJE010000000
VERSION     DACTJE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15735
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.063"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(62..763)
                     /gene="flgA"
                     /locus_tag="I9760_001554"
     CDS             complement(62..763)
                     /gene="flgA"
                     /locus_tag="I9760_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAT5043326.1"
                     /translation="MKKCILSFFLFFASTSLFSESSQSLELLKNKIEQYALNELSNYT
                     EGKIQVTADNIDPRLNLKVCDDNKLEVFNPYQTPMLNTSTMGIKCLEENNHWTLYIPV
                     KITIFKSVLVAKRALLKGTKISNSDIYQTELDVQKLKQGYFTDSKELIGLVCKHDITP
                     NSPLNPFNIELAKLVHKGEQVSIIAAHDNLTVSMDGIAMDEGALGDSVKVKNLSSKRI
                     IEAQVTGKKTVKVIF"
     gene            850..1257
                     /locus_tag="I9760_001555"
     CDS             850..1257
                     /locus_tag="I9760_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HAT5043327.1"
                     /translation="MRPCYFVCILALFMFAMPGYSENHHPQEFLKSIAGTKDEGEKIF
                     NHFCVNCHGTKPMISLGAPEIGNERAWGARLKQGMEVLFKHTDEGFNAMPPRGGCFEC
                     TDKQLILAIIAMVPKQAQKGLLNNLLDHKEYKQ"
     gene            1334..1891
                     /locus_tag="I9760_001556"
     CDS             1334..1891
                     /locus_tag="I9760_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAT5043328.1"
                     /translation="MKKQILLVPVCVLAASCASMDRSTAIVDDAGYTHYTMSMESDRK
                     GSNYFPEKRQATGKKVFIFDPKATAWAAYDAQGNRVKTGSASGGKDFCEDVGRPCRTV
                     TGSFKVYSKKGEECTSSIYPIETGGGAKMPYCMHFHGGYSIHAAYEVPNYNASHGCIR
                     VLPSAAKWLNQNFIDVGTSVIVKPY"
     gene            2160..2930
                     /locus_tag="I9760_001557"
     CDS             2160..2930
                     /locus_tag="I9760_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HAT5043329.1"
                     /translation="MILCIDVGNSHIYGGVFDGDEIKLRFRHTSKVSTSDELGIFLKS
                     VLRENNCSPETIRKIAICSVVPQVDYSLRSACVKYFSIDPFLLQAGVKTGLNIKYRNP
                     VEVGADRIANAIAATHSFPNQNIIVIDFGTATTFCAISHKKAYLGGAILPGLRLSADA
                     LSKNTAKLPSVEIIKTESVVGRSTIESIQSGVYYGVLGACKELIQRIHHEAFNGDKIL
                     ILATGGFASLFDKQGLYDHLVPDLVLQGIRLAAMMNTA"
     gene            3543..4001
                     /gene="mraZ"
                     /locus_tag="I9760_001558"
     CDS             3543..4001
                     /gene="mraZ"
                     /locus_tag="I9760_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAT5043330.1"
                     /translation="MFRGINAITIDTKGRLAIPTRYRSALGAEDKIPLVVTIDTEETC
                     LLLYTAAQWQIIEDNLQKLPSFNAAARRIQRLLIGHATDVEVDANGRVLLPTVLRNYA
                     KLEKDVVMIGQGNKFEVWNKELWESKREQWLAEEASMTDGLPEEMKTFSL"
     gene            4013..4939
                     /gene="rsmH"
                     /locus_tag="I9760_001559"
     CDS             4013..4939
                     /gene="rsmH"
                     /locus_tag="I9760_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAT5043331.1"
                     /translation="MAKHQSVLLHESIKGLAIKADGIYFDGTFGRGGHSREILNHLSD
                     KGRLFAIDKDLDAVQYAKDYFGLDKRFQIFHGSFAQIKEFASQAGVIGAVDGILLDLG
                     VSSPQLDNPERGFSFMLQGPLDMRMDLTQSINAANFVNEAEVNELAHVFRAYGEERFA
                     GRIAKAIVDARKLKPITTTLELAEIVKEANPKWEKHKHPATRVFQAIRIHVNQELTDL
                     SNCLEQCLDVLGPRGRLAVISFHSLEDRIVKQFMRDKEQGNRPPVEVPIKYEELKTNF
                     KRVGKAVKPQSSEIKENVRSRSAVLRIGEKLA"
     gene            4936..5274
                     /gene="ftsL"
                     /locus_tag="I9760_001560"
     CDS             4936..5274
                     /gene="ftsL"
                     /locus_tag="I9760_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAT5043332.1"
                     /translation="MNAAAKVINQGNLFNGQLADMQMSKSLYMLIVLLAAVLVSAFAV
                     IYSTNSYRLTLSQVQQEEQQTHFLQLQWGQLLLEQASLATPARVEELAREKLGMTLPT
                     SANTYLLHPQ"
     gene            5656..7317
                     /locus_tag="I9760_001561"
     CDS             5656..7317
                     /locus_tag="I9760_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAT5043333.1"
                     /translation="MKKTHHFARLVTVAVFFSLILAVLIWRMVDLTVLHRQFLQGQGD
                     ARSIRVIDIPAYRGMITDRNGTPLAVSTPVESVWVNPKEFSPDEEQFMQLAKYLNLAP
                     KELSRKIVRAENREFVYLQRQLPPPLSKKIEALKIPGVNFQKEFKRYYPDSDSISPLI
                     GFTNVDDQGLEGIELAYQDWLMGVVGKKRVIKDRLGRVVEDLGVLKEPRPGHELTLSI
                     DRRLQYLAYSELGKTVEEFSAKSGSVVVIDAENGEILAVANVPSYNPNARGHYDKETY
                     RNRAFTDTFEPGSVIKSFSIASALETGLFTPDTIIDTNPGWMTVHGRTVRDIHNYGVL
                     DVTGILQHSSNVGVTKMVLSTPAEQLIGFLQKCGFGQRSELAYPGESEGGIVKAKDAN
                     PFVLATLGFGYGLSVTALQLAKANLIFANKGRLIPVTLIHNDTPSNGIQVMQPKTAEN
                     VLLMMEAVMNKGTGKGARVPGYRVAGKTGTARVAGKNGYKDRKYTASFIGIAPVSKPK
                     FVVAVIIHEPSRKGYYAASVAGPLFAKVMSGALRLFNIPTDEPVS"
     gene            7339..8790
                     /locus_tag="I9760_001562"
     CDS             7339..8790
                     /locus_tag="I9760_001562"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAT5043334.1"
                     /translation="MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFVAYPGAAS
                     DGRLYIEKAVASGAVAVVYEPNNLPQGITLPTAIPCIAIPQLAEKLGEIAKRFFNNPS
                     QFLGLTGVTGTNGKTTIAYQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALC
                     LQRLMHQYKEEGIKQVCMEVSSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYG
                     AAKSRLFERAELQWAIINQDDDFQDLMSNVLKTHVKKVTYGMHQNCDVKALQWNMDIT
                     GTEIEVKSPWGHQLLKIKALGKFNIYNSLAVYSSLLASGHNPEKVVQVMAELKAAPGR
                     MEIVAQSPYVLVDYAHTPDALENVLITLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAA
                     SMYADNIVITSDNPRSEDPEIILNEIACGIPASAPVSKLVNREEAIAYALRKAEHNDI
                     ILIAGKGHESYQQIGNTKHVFSDQEVVRRLMNN"
     gene            complement(8835..9887)
                     /locus_tag="I9760_001563"
     CDS             complement(8835..9887)
                     /locus_tag="I9760_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HAT5043335.1"
                     /translation="MNILADALLPGLDSAFPPPFTVTLYHKADEIPELLHHKDVLLCR
                     STLKINGDLLKNHQIKVVATATSGTDHIDFPFLESQNISIIDAKGCNAISVADYVVAC
                     LAYLDKQQLIQGKTAGIIGLGQVGTKVYERLNAAEFQLCLYDPPKATRDTSFQSCSLE
                     DLFECDLLCVHAELHSDAPYPSLNLINRDFLKELKPGCIIINASRGGIVNEEALLHLG
                     SAILYCTDVYNNEPHIDSRIVSKATLCTPHIAGHSLEAKFAAVAIVSRKLHQMLGLPY
                     PQFATPEKPYRLNENSDWRELALSIYNPIHETLELKHAGNLSSAFLTLRKNHHHRHDF
                     TTYFDSDSIKKYPLLG"
     gene            complement(9884..10543)
                     /locus_tag="I9760_001564"
     CDS             complement(9884..10543)
                     /locus_tag="I9760_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HAT5043336.1"
                     /translation="MPEFTLIQQICIWALPVLLAITFHELAHGYVAYRCGDNTAKMFG
                     RLTLNPIKHIDPIGTVLLPLLIGVITNFNFVIGYAKPVPVNQSQLRKPRRDMILVTLA
                     GPLANLIMAFLWAGCFKIATFLNPQSSMAALFLLVTARAGMMINLILAFLNLIPIPPL
                     DGSKVVINLLPARQAIAYAKLEPFGFLILIVLIFTNVLGYILTPLIYGSLYILSAIFN
                     L"
     gene            10800..11438
                     /locus_tag="I9760_001565"
     CDS             10800..11438
                     /locus_tag="I9760_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAT5043337.1"
                     /translation="MTQFEKTFNFMKKIWVIITYIILVVLSYYFLDRTLATYFHSLDL
                     RVNAHALTYLTALGKWKIYVALFLITALYFRYIQQNKQYEIRSWYLLGCVLLPNLLTF
                     VLKISFSRARPDLLFDNNLYGFYWFQHNDNYWSFPSGHSITITALAAGLGFLFPRYFF
                     LFIGAALLVAATRVILYHHYLSDVMTGFYISMLLVGLFTQYLQRNHYLNKIN"
     gene            11478..12722
                     /locus_tag="I9760_001566"
     CDS             11478..12722
                     /locus_tag="I9760_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAT5043338.1"
                     /translation="MTIERELKNFVRLYAEWDTTNNCYKSHLDKDTLLRLERDFARFI
                     NQQLLIDGPEKEKIVKNYKRLTLCFHPDHASGFSPEMVWLENNLSQSKNNGACFKILG
                     LCYEKLISPEKFKDSSLGDIKSKDDFKQWLENLRSKATTYSGQSFCDSLINLLEQSSG
                     FFDDTGKIKPRGLRVLLRFIPVVFASYGTFLFAEELFAIYALYFILLKGGQYMESTES
                     SQLKKIGCTLQEISIITATASTTLLVRLLEMTFWASRQFLDVSLQIGSAILKPMLPAP
                     ETKEKSESDTAANLCRDLILASHNTGEGMQFKTPELKVISAPLESYLGLNAQQFFGDL
                     RIGREKRLKVEAFLFKMRVLDSLPSPLEEKLVEAQKELDKIKKDTKVFTSNTAKAVKE
                     AEQVISLLQDKDSMQLVVYNGT"
     gene            13131..13880
                     /locus_tag="I9760_001567"
     CDS             13131..13880
                     /locus_tag="I9760_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAT5043339.1"
                     /translation="MKILVAVKRVIDPYVKIRVKSDNSGVETQNIKMAMNPFDEIAIE
                     EALRLREKNWATEVIAVSIGADSSQETLRHALALGADKAILVRTEKSFESLNIAKILN
                     NIVANEKPDLVLMGKQSIDGDNNQTPQMLAALLNWPQATNASKIIPDNNNLEVVREID
                     GGLETLKVQLPAVISTDLRLNEPRYASLPNIMKAKRKPLDIIDLDSLGLSLKQHVEIL
                     KVNAPATRSGGVILDSVAALLDKLQHEAKVL"
     gene            13894..14832
                     /locus_tag="I9760_001568"
     CDS             13894..14832
                     /locus_tag="I9760_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAT5043340.1"
                     /translation="MSTLVLVEHNNQQLHPATRNTLSAALELGDKITLLVVGYQCRSV
                     AEEAASLEGAHAVLHVDNPCYEHQLAEAVSDLVVSFANSFSAILAPSSTYGKNILPRV
                     AAQLDVTQISDVSKIINSDTFEHPVYAGNAIETVRVLDSLKVMTIRTTAFDPITSSQE
                     VCCIESIDKEFQAQGSQFVRHELSKSERPDLGSAKIVVSGGRGLQNAEKFKLIEELAD
                     TLGAAVGASRAAVDAGFVPNDYQVGQTGKVVAPMLYVAVGISGAVQHLAGMKDSKVIV
                     AINKDADAPIFQIATYGLVGDLFELVPQLIEQLKNR"
     gene            14844..>15735
                     /gene="ald"
                     /locus_tag="I9760_001569"
     CDS             14844..>15735
                     /gene="ald"
                     /locus_tag="I9760_001569"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HAT5043341.1"
                     /translation="MLVGVPKEIKSHENRVGLVPSSVREIIRAGSDVLVEKGAGLGIG
                     ISDDDYRNAGAEVVDTADDVFANAELIVKVKEPQPIECKRLREGQTLFTYLHLAPDPH
                     QARLLKESGVTAIAYETVTQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILL
                     GGVPGVAAANVVVIGGGVVGTNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTV
                     YSTADSIEKYVSSADLVIGAVLVPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFE
                     TSRATTHQEPTYVVDNVVHYC"
BASE COUNT         4862 a         2662 c         3407 g         4804 t
ORIGIN      
        1 gtaaattaag gttgattttt aatccttttt ccaaattttt tcttaaaaat taaaacatta
       61 gttaaaaaat aactttcacg gttttctttc ctgtgacttg agcttcaata attcgttttg
      121 aggaaagatt ttttactttt acagagtctc ctagcgctcc ctcatccatt gcaattccat
      181 ccatactcac tgttaagttg tcatgagcgg ctattataga tacctgttct cctttatgga
      241 ccagtttggc taattcaata ttgaaagggt ttagtgggct attaggcgtg atatcatgct
      301 tgcaaaccaa tccaataagc tctttactgt cagtaaaata tccttgtttc agtttttgaa
      361 catcaagctc ggtttgatat atatctgaat tactaatttt tgtacctttc agtaaagctc
      421 gttttgccac aagtacagat ttaaatatag ttattttgac aggtatatat aaggtccagt
      481 gattgttttc ctccaaacat ttgattccca tggtactggt attgagcatg ggtgtttgat
      541 aagggttaaa tacttcaagt ttattatcat cacaaacttt taagtttagg cgaggatcaa
      601 tattatccgc cgtaacctga attttacctt cagtatagtt tgataattca ttgagggcgt
      661 attgttctat tttatttttt aataattcga gagactgact tgattctgaa aataaactgg
      721 tgcttgcaaa aaataagaaa aagcttagta tgcacttttt cacgacataa actcccttag
      781 tctattgatt ttttatattg aaacgcagcg tgtcccaagc aagtattgcg ccaattttag
      841 gagtatatca tgagaccttg ttattttgtt tgtatattgg ctttatttat gtttgcgatg
      901 ccagggtact ctgagaatca tcatcctcaa gagtttctaa agtcaattgc tggcacaaag
      961 gatgaagggg aaaaaatttt taatcatttt tgtgttaatt gccatggaac gaaaccaatg
     1021 atttctttag gagcccctga aattggaaat gaaagagcat ggggggcaag attgaaacaa
     1081 ggaatggaag tgctttttaa acacaccgat gaagggttta atgccatgcc tcccagagga
     1141 ggatgctttg aatgtactga taaacagctg attctagcta tcattgcgat ggttcccaaa
     1201 caggctcaaa aaggactatt aaacaacctg ttagatcata aagaatacaa acagtaaaaa
     1261 atttttataa aaaagcctaa acttattcaa actgctcccg atatagttaa aaagagaaag
     1321 agggagatag aaagtgaaaa aacaaatact gttagttccg gtttgtgtct tggctgcctc
     1381 ttgtgcatcc atggatagat caacggccat agtcgatgat gcgggttata cccattacac
     1441 tatgagtatg gagtccgata gaaagggttc taattacttt cctgagaaaa gacaggccac
     1501 aggaaaaaaa gtatttattt ttgatcctaa agctactgcc tgggcagctt acgatgctca
     1561 gggtaaccga gttaaaaccg gtagtgcttc agggggaaaa gatttttgtg aggacgttgg
     1621 cagaccctgt cggacggtaa caggcagctt taaagtttat tccaagaaag gcgaggagtg
     1681 tacctccagt atctatccaa tagaaactgg cggtggagca aaaatgcctt attgcatgca
     1741 ttttcatggt ggttattcaa tacatgctgc ctatgaagtt cccaactata acgcaagtca
     1801 cggctgtatc cgggttttac caagcgctgc aaaatggtta aatcaaaact ttattgacgt
     1861 aggaacttcg gtaatcgtta aaccttatta ataagtaaaa attctccact actccccgct
     1921 aattgcgggg tttttttatt ggatgatttt attatctcgt gcagacaaga ggataatggt
     1981 ttcgtaagaa gtccagctaa agtttgggtt gggagcaaag cggatctaac aataatttga
     2041 atcaataata tatagaatgc tctcaataca gggctatttc tttccgggat tgctgaatta
     2101 ttatcttaga cctataatca caaatcacta gttatgccat ttaaaaagta gatttaataa
     2161 tgattctgtg tattgatgta ggaaactctc atatatatgg tggagtattt gatggagatg
     2221 agattaaact ccggtttcgc catacttcca aggtaagtac ttcggatgaa ttgggcatct
     2281 ttcttaaaag tgttttacgt gaaaacaatt gctctcccga gacaattaga aaaattgcta
     2341 tttgttcagt agtacctcaa gtagattact cattgcggtc agcctgtgtg aagtattttt
     2401 ctattgaccc gtttttatta caagcaggag ttaagactgg attgaatatc aaatatagaa
     2461 atccggttga ggttggagct gaccgaattg caaatgctat tgccgccact catagttttc
     2521 ccaatcaaaa cattattgtt attgattttg gtacagcaac tactttttgt gccatttctc
     2581 ataaaaaagc ttatttaggt ggagctattt tacctggtct tagattatct gctgatgctt
     2641 tgtcaaaaaa tactgcaaag ctaccttctg ttgaaattat caagaccgag agtgttgttg
     2701 ggcgttctac catagaaagc attcagtctg gagtctacta tggtgtcctt ggtgcatgta
     2761 aagaattaat tcaaagaata catcatgaag cgttcaatgg cgataaaata ctaattttgg
     2821 caacaggagg atttgcgtct ttatttgata agcaaggtct ttatgaccat cttgtgccag
     2881 atttggttct ccaggggata agattagctg ctatgatgaa tactgcctga gcagtaaaag
     2941 gtttttgtta atatcagatt tatttctgtt tattctaatg gagtttaagg ggtgttttgt
     3001 attctggtaa gttaatcaaa agagaggatt tcgagtctta taaagcagga aacccttttg
     3061 ctcaggattt ccatttttag cgttttacct cccgcttttt ctattagaaa aactctgggc
     3121 tcttccttct gtttcattaa tcaattcaag atcaacctat gaaccaaaca aattctagac
     3181 cgctcacttg cataatctgt ctctttcatt ttattgctgc gttggaaatg ttcttgtggg
     3241 gctcatttac tctggcaaac cctgctcctc aaaaaatctg ctctttcctt gaagcaaaat
     3301 aatcagatga ttcgagagaa cttataactg tgagcataca tttggttaca aatctttctt
     3361 aaagatcaaa taaataacaa gtttgggatt tgcctcttta atccataaaa aaaattactc
     3421 caaaagtggt atttcagctt gacgtgtgta gaaatgtgtt cctatagtgt agaaaagtgg
     3481 gttaaaataa taaaaaagtg gagaattgtg ggagaataat tctccttcac ttgggaaata
     3541 atatgtttcg tggaattaat gccatcacca tagacactaa agggcgctta gctattccga
     3601 cgcgttacag aagtgccttg ggtgcagagg ataaaatccc tctcgtggtg actattgata
     3661 ccgaagaaac ctgcttactc ctttacacag cagcacaatg gcaaattatc gaagacaatt
     3721 tgcaaaagct accaagtttt aatgctgcag caagacgaat tcaacgtctg ttaatcgggc
     3781 acgccactga tgtggaggtt gatgcgaatg gacgagtact tttacccaca gttttaagaa
     3841 attatgcaaa gttagaaaaa gatgtcgtga tgataggcca gggaaataaa tttgaggttt
     3901 ggaataaaga attatgggaa tccaagcgtg agcaatggct agcagaagaa gcgtccatga
     3961 cggatgggtt acccgaggaa atgaaaacgt tttctttata acggaaagag aaatggcaaa
     4021 gcatcaatct gttttattac atgagtcaat caaaggtttg gctataaaag ctgacggcat
     4081 ctattttgat ggaacttttg gccgaggagg ccacagccgc gagattttaa atcatttaag
     4141 cgataaagga agactgtttg caatagataa ggaccttgat gcggtgcaat atgccaagga
     4201 ttattttggt ttggataaac gatttcaaat ttttcacggt tcattcgcgc aaatcaagga
     4261 atttgcgtca caagcgggag taattggggc cgttgatggt attttgcttg atctgggagt
     4321 gtcttcacct cagttggaca atccagaaag aggttttagt tttatgctgc aaggccctct
     4381 tgatatgcga atggatttaa ctcaatcaat taatgctgcg aattttgtca atgaagcaga
     4441 agtcaacgag ctggctcatg tattcagggc ttatggtgag gaacgatttg caggcaggat
     4501 tgcaaaagct attgttgatg caagaaaact aaaaccaata acaactactt tggagttagc
     4561 agaaattgtt aaagaagcaa accccaaatg ggaaaaacac aaacatcctg caacccgggt
     4621 atttcaagcg attcgaatac atgtcaatca ggaattaacc gatttgagta attgtctaga
     4681 gcaatgtctg gacgtattgg ggccaagagg acgcttggcc gttatcagtt tccactcttt
     4741 ggaagaccga atagtcaaac aatttatgag agataaagag caaggtaaca ggccgcctgt
     4801 tgaagtccca atcaaatacg aagaactaaa gaccaatttt aaaagagtag gtaaagcagt
     4861 gaaaccacag agtagcgaaa taaaggaaaa tgttagatct cgaagtgcag tacttaggat
     4921 aggggagaaa ttagcatgaa cgctgcagcg aaagtcatta atcaaggtaa tttatttaat
     4981 ggtcaattgg cagatatgca aatgtcaaaa tcactctata tgttgatagt attgttagct
     5041 gccgtactag tcagtgcatt tgctgtcata tatagcacta attcctacag attaactctc
     5101 agtcaagtgc aacaagagga acagcaaacc cactttttac aattgcaatg gggacaatta
     5161 ttattggaac aggccagttt ggcaactcca gcccgggttg aggaattagc cagagaaaaa
     5221 ttgggaatga ctttaccaac ttctgcaaat acgtacttac tgcatcctca ataagtgagt
     5281 cgttttagag gagttaaagt gtttccatat tttgagttat aggtaagagc tgagtacctg
     5341 gtgaaggata atctatgtcc aagagcatca aattaaagat tgaataatga aatttcaaag
     5401 gatttgatag agcaattagg cgcaatgcgg aggaaatcac ctcgaaaaaa ttgaaagctt
     5461 catgtacact tacacgtcat tcatcttctt tccaggacgt catggaagac taagtgtaaa
     5521 atagcctgat aaattccagg caggtatacg tgccttatgt ttaactctaa ggtaattttg
     5581 agaaagaggt ttcaagtata ttttcactaa tttattgttg cgtgcatagc taatactatt
     5641 tatgagtagt catatatgaa aaaaacacac cattttgcca ggctggttac tgtcgctgtt
     5701 tttttctctt tgattttggc tgttttgata tggagaatgg ttgatctcac tgttctgcat
     5761 aggcaatttt tgcagggcca gggtgatgcg cgcagcatcc gagttataga tattcctgcc
     5821 tatcgaggta tgatcaccga tagaaatggt actccgcttg ctgtgagcac acctgttgaa
     5881 tcggtttggg tcaatccaaa ggaattttct ccggatgaag aacaatttat gcagttagct
     5941 aaatacctga atttggcacc taaagaatta agtagaaaaa ttgtccgggc cgaaaatcgg
     6001 gagtttgtgt atttacaaag acagttacct ccaccccttt cgaaaaagat cgaagcttta
     6061 aaaatcccag gtgttaattt tcaaaaagaa ttcaaacgtt attatccaga ttcagatagt
     6121 atttccccat taattggatt taccaatgtc gatgatcaag gattggaggg gattgagctg
     6181 gcctatcaag attggcttat gggagtcgtt ggtaaaaaaa gagtaatcaa agatcgatta
     6241 ggacgagttg tcgaagatct gggagttttg aaggaaccac gcccgggaca tgagttaact
     6301 ttgagtatag atagacgact acaatattta gcttatagcg aattaggtaa aacagttgaa
     6361 gagttttctg ctaaatctgg gtctgtagtt gtaatcgatg ctgaaaatgg tgagatcctg
     6421 gctgtggcta atgttccatc ttataatcct aacgcacgtg ggcattatga taaggaaacc
     6481 taccgcaaca gagccttcac cgatactttc gagcctgggt ctgtcattaa atcattcagt
     6541 atagccagtg ctttggaaac aggcttattt actccggata ccattattga tactaatcca
     6601 ggatggatga ctgtccatgg ccgaacagta agagatatcc ataattatgg tgttttagat
     6661 gttaccggaa tattacaaca ttccagtaat gtgggtgtta caaaaatggt attatccact
     6721 ccagctgaac aattaatcgg gtttttgcaa aaatgtggtt ttgggcaacg ctctgaattg
     6781 gcatacccgg gtgaaagtga aggaggcata gtaaaagcca aggatgcaaa cccattcgtt
     6841 ttagccacat taggtttcgg ttatggcttg tctgttactg cactgcaatt ggctaaagcc
     6901 aatttaattt ttgcaaataa aggacgatta atacctgtga ctttgattca caatgatacc
     6961 cccagtaatg gcattcaagt gatgcaacct aaaaccgctg aaaatgtatt attaatgatg
     7021 gaagcggtaa tgaataaggg tacaggtaag ggagcaaggg ttcctggtta tagggtagct
     7081 ggaaaaactg gaacagcacg agttgctgga aagaatggat ataaagacag gaaatacaca
     7141 gccagtttta ttgggatagc accagtttcc aaaccaaagt ttgtagtcgc tgttattatt
     7201 catgaacctt cacgaaaggg ttattatgct gcctctgtgg cagggccctt atttgctaaa
     7261 gttatgtcag gtgctttacg cttattcaat attcctactg atgagccagt tagttaaaga
     7321 agaataggag ccaattttgt gaaattaacc caattattag aaccgtggat taaaagggac
     7381 attattgatg gcactgtgag tgacataaaa aatgacagcc gcttggtgaa agaaggggat
     7441 ttatttgtcg cctatccagg ggcagcttcc gatggtcgat tatatataga aaaggccgtg
     7501 gcatcaggtg ctgtcgctgt ggtttatgag cccaataatt tacctcaggg aattactttg
     7561 ccaacagcaa ttccctgtat agccattccc caattagctg aaaaattagg ggaaatagcg
     7621 aaacggtttt ttaataaccc atcccaattt cttggtttaa ctggggtaac tggcacaaat
     7681 ggcaagacga ctattgctta tcagttggca caggcacatg atttactcgg gcagcactcg
     7741 gcttacatcg ggactatagg acaaggcaag gtggatgctt taaaaccttt ggaaaatact
     7801 acgccagatg ctctttgttt gcagaggttg atgcaccagt acaaagaaga gggcattaaa
     7861 caggtttgta tggaggtatc ttctcacgcg ttgagccagc atagagtcga ctctctcgaa
     7921 tttagtcagg ctatctttac caatttaacg ctcgatcatt tagattatca ccaaactatg
     7981 caagcttatg gggctgcgaa gtcgaggttg tttgaacgag cagagttgca atgggctatt
     8041 attaatcagg atgacgactt tcaagatctc atgagcaatg tgctaaagac tcatgtaaaa
     8101 aaagtgactt acggtatgca tcaaaattgc gatgtgaaag cgctccaatg gaatatggat
     8161 atcaccggaa cagaaattga agtcaaatct ccttgggggc accagcttct aaagattaaa
     8221 gcactgggta agtttaatat ttataatagc ctggctgttt atagcagttt gcttgccagc
     8281 gggcataatc cagaaaaagt agtgcaagtt atggccgaat taaaagcggc tcctggccga
     8341 atggaaattg tcgctcaatc cccatatgtt cttgttgatt atgctcatac tcccgatgca
     8401 ttggaaaatg ttcttatcac cttaaaacaa ataaaaaaag ggagattgtg ggttgtattt
     8461 ggttgtggtg gggacagaga taaaaccaaa cggcccatta tgggaaaggc agccagcatg
     8521 tacgctgata atattgtcat taccagcgat aatccgagaa gtgaagatcc ggaaattatt
     8581 ctaaatgaaa ttgcttgtgg cattcctgcc tctgcgcctg tctctaaact ggtgaataga
     8641 gaagaggcaa ttgcttatgc tttaagaaag gctgagcata acgatatcat attaatagca
     8701 ggaaaaggac atgaatcgta tcagcagatt gggaatacaa aacatgtttt ttctgatcag
     8761 gaagttgtaa gacgtttgat gaataattaa aatttcggga caggggttaa ttattagaaa
     8821 cagacatcaa cactttaacc taataatgga tactttttta ttgagtcaga atcaaaataa
     8881 gttgtgaaat catggcggtg atgatgattc ttgcgaagag tcaagaatgc tgaggataga
     8941 tttccagcgt gttttaattc tagtgtttca tgaatcgggt tatatattga taaagccaac
     9001 tctctccagt cgctgttttc atttaaccta taaggttttt caggggttgc aaattgtggg
     9061 tatggaagac caagcatttg atgtaattta cgactgacga ttgcgactgc agcaaattta
     9121 gcttcgaggc tgtgtcctgc aatatgtggt gtgcaaagag ttgccttgga gacaatccgg
     9181 ctatctatat gaggttcgtt attgtagacg tcggtgcaat aaagtatggc agagccaaga
     9241 tgcaaaaggg cttcttcatt aacgatacct cctcgagaag cattgatgat aatgcagcca
     9301 ggttttaatt ccttgagaaa atcccgatta attaagttaa ggcttgggta gggcgcatcg
     9361 ctatgtaatt cagcatgaac acataagagg tcacattcaa aaagatcttc aagagagcag
     9421 ctttgaaaag aagtatctct ggttgctttg ggtgggtcat aaaggcataa ctgaaattcg
     9481 gcagcattta gtcgttcata aactttagta cctacttgcc ctaaaccaat aattcctgct
     9541 gttttacctt gaataagttg ctgtttatct aaataggcaa ggcaagcaac aacatagtca
     9601 gcaaccgata tggcattgca tcctttggca tcaataatag atatgttttg tgattctaaa
     9661 aaaggaaaat caatatgatc ggttccactg gttgctgtag caacaacctt tatttgatgg
     9721 ttttttagta aatcgccatt gatttttaga gttgaacgac aaagaagaac gtccttatga
     9781 tgaagcaatt ctggaatttc atcggcctta tgatacaagg ttacggtaaa tggtggtgga
     9841 aaagcactat ccaatcctgg caataaagca tctgctaaaa tattcataaa ttaaaaatcg
     9901 cgcttaaaat atataatgaa ccatagatga ggggagtaag aatatatccc aatacattag
     9961 taaaaattaa tacaatcaat attaaaaaac caaatggttc taattttgca taagcgatag
    10021 cttgtcttgc tggcaataga tttattacaa ctttgctgcc atcaagtggc ggaatgggga
    10081 ttaagtttaa aaaggcaaga atcaaattga tcatcattcc cgctctggct gtaacaagca
    10141 ggaataaagc agccattgag ctttgaggat ttaagaaagt tgcaatttta aaacatccag
    10201 cccataggaa tgccattatc agatttgcta atggtcccgc aagcgttacc aatatcatat
    10261 ctcggcgtgg ttttcgcagt tggctctggt taaccggaac cggttttgcg taaccaatta
    10321 cgaaattgaa atttgttatc acgccaatta aaagaggtaa tagaacagtg cctattggat
    10381 caatgtgttt gatagggttt aatgttaatc gaccgaacat tttagctgtg ttgtcaccgc
    10441 agcggtaggc aacgtaacca tgagctaact catgaaaagt aatagctaat aatactggta
    10501 aagcccaaat acaaatttgt tggattaacg tgaattctgg cattaatatt ctgatgtatt
    10561 taataaggat cctgatttta acgaattcta ctcgctaata acaagggttg atgaaaaatt
    10621 gtgtggccgc tcgcatttgt tgatattttt accatccttg ccgaaaaatc ttgattttct
    10681 actcaagcgt gagaaatctc gacaggacct aatttttatt aataacatgg ggtcttggct
    10741 aaatcgaaag catcttatat gattccattt gatatgagga tttataaggc ttggtttcga
    10801 tgacgcaatt tgagaaaact tttaacttca tgaaaaaaat ctgggtaata ataacctata
    10861 ttatccttgt tgttctctct tattattttt tggatagaac gttggctact tatttccata
    10921 gcctggatct tcgagtcaat gctcatgcat taacatactt gacggctttg ggaaaatgga
    10981 agatttatgt tgcattgttt ttaatcaccg cattatactt tcgatacatt cagcaaaata
    11041 aacaatatga aattagatct tggtatttat tgggctgtgt tttgctgcct aatttattga
    11101 cttttgtgtt aaaaattagc tttagtcgtg cgcggccgga tctcttgttt gataataacc
    11161 tctatgggtt ttattggttt caacacaatg acaattattg gtcattccct tcagggcatt
    11221 ctattactat aactgctttg gctgcgggat tggggtttct cttcccaaga tattttttcc
    11281 tctttatcgg agcagccttg cttgtcgctg ctaccagggt tattttatat catcattatt
    11341 taagtgatgt tatgactgga ttttatatca gtatgttgct tgttggctta tttacacagt
    11401 atttgcaaag gaaccactat ctcaataaga taaattagac tctataaatc gtagtcaaat
    11461 ttattaaggg agtaattatg actatagaaa gggaattaaa aaattttgta agattgtatg
    11521 cggaatggga cacaactaat aattgttata aatcccattt agataaggat accttattga
    11581 gattagaaag agattttgca agatttatta accagcaatt attgatagat gggccagaaa
    11641 aagaaaaaat tgttaaaaac tataaaagac taactctctg ctttcatccc gatcatgctt
    11701 caggattttc gccagaaatg gtttggctag agaataattt atctcagagc aagaataatg
    11761 gagcatgctt caagatactt ggcttgtgct atgagaaatt aatttcacct gaaaaattca
    11821 aagatagtag tttaggagat atcaaaagca aggatgattt taaacaatgg ctggaaaatt
    11881 taagatccaa ggcaactacc tattctggcc agagcttttg tgacagttta attaatttat
    11941 tagaacaatc cagtggtttt tttgatgata ctggcaaaat aaaacctaga ggactcagag
    12001 ttttacttcg atttattcca gtagtatttg ccagctatgg cacttttctt tttgcagaag
    12061 aattgtttgc tatctatgcg ctttatttta ttttattgaa aggagggcag tacatggaga
    12121 gcactgaatc cagtcaatta aaaaaaatag gctgcacgtt gcaagaaatc agtattatta
    12181 ctgcaactgc atcaactact ctattagttc gcctgcttga aatgactttt tgggcttcgc
    12241 gccaatttct tgatgtgagt ttacaaatcg gctctgcgat tttaaaacct atgttacccg
    12301 ctcctgagac taaggaaaaa tcagagtctg atacagctgc caatttatgt agagatttaa
    12361 tcttggctag ccataatact ggtgaaggga tgcaattcaa gacaccggag ttaaaagtca
    12421 tttctgctcc tcttgaatct tatcttggtt taaacgcgca gcaatttttt ggtgacttga
    12481 ggataggtcg agagaaacgt ttaaaagtag aggcattttt attcaagatg agagtactgg
    12541 attctcttcc tagtcctttg gaggagaaat tggtagaagc tcaaaaagaa ctggataaga
    12601 taaagaagga tactaaggtt tttacaagca atacagcaaa agccgtaaaa gaagcagaac
    12661 aagttatctc tctgctacaa gacaaggatt ctatgcaatt ggtggtttat aatgggactt
    12721 aatagctgtt gcaagtatct catgcttatt ctcagggcaa aatcacacat tggttaaata
    12781 acggattatg tgtgtgattg gtgtgtatga gtgaagctat tgcaaatctc gatcgtcagt
    12841 ttgctataaa aacagcttcc ttacctatgc gtgaatgaca aaatataacc attgtttgca
    12901 ctgttaattg tgcttaaggg cattattttg ccctatgttt tttctttttg catgagtaga
    12961 gttttccaac ccggaccaga ttataaggca ggtgagaata gaaaaaggtt ttctttaaca
    13021 tagctcattt agaataaatt agctttatgt tctaattaaa atcaacctct tgaatttata
    13081 cccctatctc tggcaagatg cagactgatt tgatattcca gtgaggaacc atgaaaatac
    13141 tcgtggctgt aaaacgtgtg attgaccctt atgtcaaaat tcgagtgaaa tcggataatt
    13201 caggagttga aactcaaaat atcaaaatgg caatgaaccc atttgatgaa atagccattg
    13261 aagaggcatt gcgtttgcga gaaaaaaatt gggcaacgga agttattgct gtaagcattg
    13321 gtgcggatag ctctcaggaa actttaaggc acgctttagc tttgggcgcg gataaggcca
    13381 ttctggttcg aacagaaaaa tcattcgaga gccttaatat agccaagata ttaaataata
    13441 tcgtagctaa tgaaaaaccg gatttggttt tgatgggcaa acaatcgata gatggtgaca
    13501 acaatcaaac tcctcaaatg ttggctgcat tattgaattg gccacaggct accaatgcgt
    13561 cgaagatcat tcccgataat aataatcttg aagtggtgag agaaattgat ggtggtttgg
    13621 aaacactaaa ggtacagctg cctgcagtaa tcagtaccga tcttcgttta aatgagccca
    13681 gatatgccag cttgccgaat attatgaagg ccaaacgaaa gccccttgat atcattgatc
    13741 tggattcgct tggcttatct cttaaacaac acgttgaaat attaaaggtt aatgccccgg
    13801 caacgagaag tggtggtgtc atacttgatt ctgtggctgc attgttggat aaattacaac
    13861 atgaagccaa agtgctttaa ttaaggagat gagatgagca ctttagtttt agttgaacat
    13921 aataatcagc agttacatcc agcaacccgc aatacactgt ctgctgcttt agaactcggt
    13981 gataaaatta cattactagt ggttggatat caatgccgtt cagtagccga ggaggcagcc
    14041 agtctggaag gagctcatgc tgttttgcat gtagataatc cttgttacga acatcaattg
    14101 gctgaagcag ttagcgattt ggtggtgtct tttgcgaatt cattcagtgc tattttagct
    14161 ccttccagta cttatgggaa aaatatattg cccagagttg ctgcacaact tgatgtgacc
    14221 cagatttctg atgtgagcaa gataattaat tcagacacat ttgaacatcc tgtttatgct
    14281 ggtaatgcca tagagactgt gcgtgttttg gattcattaa aagtaatgac tattagaacg
    14341 acagcttttg atcccatcac atcaagccag gaagtatgtt gcatagaaag tattgataag
    14401 gaatttcaag cccaaggaag tcaatttgtg cgccatgaat taagtaaatc cgagaggccg
    14461 gatttaggta gtgccaaaat agttgtttct ggcggtagag ggctacaaaa tgcagaaaaa
    14521 tttaaattaa tcgaagaatt agctgatact ttgggcgcag cagtaggggc atccagagct
    14581 gcagttgatg ccggttttgt tcctaatgat tatcaggtag gtcaaaccgg gaaggtagtg
    14641 gctcctatgc tttatgttgc cgtgggtatt tctggagcag ttcaacattt ggcaggtatg
    14701 aaggattcca aggttattgt tgcaattaat aaagacgcag atgcacctat ttttcaaatt
    14761 gcgacttatg ggttagtagg tgatctgttt gaattagttc cacaattaat agaacaatta
    14821 aagaatcgat aagaggtaac aatatgttag taggggtacc aaaagaaatc aaatcgcacg
    14881 aaaacagagt aggacttgtt ccctcgagtg ttagagaaat tattagggca ggaagtgatg
    14941 tattggtaga gaagggggct gggcttggta ttggcatatc ggatgatgat tatagaaatg
    15001 ccggggctga agtggttgat actgctgatg acgtatttgc caatgccgaa ttaatagtca
    15061 aagtaaaaga gccccagcct attgaatgta aacggttacg tgaagggcaa acattattca
    15121 cttatttaca tttagctcct gatcctcatc aagcgcgttt attaaaagaa tccggggtta
    15181 cagcgatagc ttatgaaact gtgacacagg atgatggcgg gttaccttta cttactccca
    15241 tgtcccaagt ggctggtcgt atggcaatcc aggccggtgc acattgcttg gagatggctc
    15301 aagggggtag cggtatattg ctaggtggtg tacctggtgt tgctgcagcc aatgtcgtag
    15361 ttattggcgg tggagttgta ggaacaaatg ctgttcgcat ggcgatgggg atggaagctc
    15421 gtgttattgt gttagatcgt tcactgcaac gtttaaatga attggatttt cagtttggtt
    15481 ctaaactcaa tacagtttac tcaactgcag attctataga aaaatacgta tccagcgctg
    15541 atttagtgat tggtgctgta ttggtaccag gtgctgcagc ccccaaatta gtgactcgct
    15601 ctatgctgag ctcaatgcgt cctggctccg ttttagtcga tgttgctatt gatcaaggag
    15661 ggtgttttga gacgagtcgt gcgacaaccc atcaagaacc tacttacgtg gttgataacg
    15721 tagtgcatta ttgtg
//
LOCUS       DACTJE010000064        15603 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.064, whole genome shotgun sequence.
ACCESSION   DACTJE010000064 DACTJE010000000
VERSION     DACTJE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15603
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.064"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..2055
                     /locus_tag="I9760_001570"
     CDS             <1..2055
                     /locus_tag="I9760_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5043342.1"
                     /translation="KLKEKLLKISSKGISSAFEGIIITDSEGTILEANQAFMKMTGYT
                     EQEVIGQKPAIFQSRKQNEKFYKELWKILAEKGQWSGELWAKKKNKKLFPAFLAISSV
                     KNSEGDVINYIGYYHDLTLIKKQEQQIENFKYYDYLTGLPNKFLLTQRLQMLISGLSE
                     ENKELIVFNIRVFDSNSKNSLLDFPPNIRDKIILEVVNRIDRTSPKKMFYSRIGLGEF
                     IVIYFIKSRIEVMASIAQRMIEKLACPYYIDHQTIILQTIIGLTAYYKNSAFSAEDLL
                     QQSEIARHKAQINGVNIFEFFDPQVEERIIEFNKRIESVSKAMQLNELKVYYQPIVEL
                     NTAKVVGLEALLRWQHPEKGLLLPKQFLSDLNNHPLSLQIGDWVLHQVLHQVEELLAC
                     NIKIPINVNVSAYQLQHPNFVKKLFDTLARYPNVPHRLLVLEILETEAIEDLTLVSQI
                     IAECKAKGINFSLDDFGTGYASLTFLKELSTSEVKIDRSFIINIFDNPKDLAILKIII
                     ESCKVIGRTLIAEGVETVAHGKLLELLGCHLIQGYAIAKPIPPAKLIIWIKNWHLGPE
                     WRETRFSQAELSNLVKISIKHYLHYQSIRLYLENNQSPKPELSLEVCPIEQWLIENQQ
                     TIKESKRFKKVCDLHHKEHEVGNTIIQLIRTGQKEQVDKQLAKLDGLRDKFLKELMNL
                     IF"
     gene            2168..2878
                     /locus_tag="I9760_001571"
     CDS             2168..2878
                     /locus_tag="I9760_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043343.1"
                     /translation="MRNLKFSHPFSIGISLFLFILFFVPLLSYGAKTSNMRGKRYCEI
                     ILSKTISSYAVYNTWGLNDCPEQLWSKVSMPAVKKETGASFVHLNGPRYWVIDGFKNT
                     SLINPAIKTISGIPMREAGILHLSLIDLFKNKPYQSHIVDRKTTWIYQEGKPVFELID
                     PTGQVFVMQSYSVQKYPQIMDNLKQLGDKLQLPKGWKFKTGVLNKLETIEAVNNKAVV
                     VQDNFLNTYQKASHDFLK"
     gene            3094..3477
                     /locus_tag="I9760_001572"
     CDS             3094..3477
                     /locus_tag="I9760_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043344.1"
                     /translation="MILRLILTSYLIIFPYKFLMANQVMNTAIKVLEECYDKTTDLRK
                     YVPCVETEADKIPALQNLQIRIKFKNPEKNSKEKVPILMVDKTGYMYYCIATAGKSLT
                     IDSCAGTQGKPLSEGQLMSIELLKD"
     gene            3758..4397
                     /gene="ravN"
                     /locus_tag="I9760_001573"
                     /pseudo
     CDS             3758..4397
                     /gene="ravN"
                     /locus_tag="I9760_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavN"
     gene            complement(4486..5181)
                     /gene="lem5"
                     /locus_tag="I9760_001574"
     CDS             complement(4486..5181)
                     /gene="lem5"
                     /locus_tag="I9760_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem5"
                     /protein_id="HAT5043345.1"
                     /translation="MVRFFKDREIEIKDSAGEYYKKLCNALKTTMSVCIASTSALVIN
                     ELSAKINKDRMTQYEENSSIAIHGIGEIRVFLLRIFKHLEDNKIDFLDGRAAIKNTMS
                     KGMAGNCEHQAFYLAALLRQQNIPALIYDIEDINHTVVISENYLLDPWIGEIYMLAHT
                     DLRPFYGSSLNMKASWLNRLLSNKKFSYPDELTLQTLKEYFVQLEDQNKIGLNILDNK
                     IGIGHQIPCELSY"
     gene            5313..5447
                     /locus_tag="I9760_001575"
     CDS             5313..5447
                     /locus_tag="I9760_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAT5043346.1"
                     /translation="MEFKMTKYRDAGTGRYVTKEFAKKHPGITIGENSKPKSQRKPKK
                     "
     gene            5686..7746
                     /gene="ravM"
                     /locus_tag="I9760_001576"
     CDS             5686..7746
                     /gene="ravM"
                     /locus_tag="I9760_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavM"
                     /protein_id="HAT5043347.1"
                     /translation="MKYFLKLSRQHSSNQKKRTQPAQFEDHHLRIRQPQYAPITIDLV
                     GSVRFIFTFSLFCFRAIAGLLTGAISRKNLFDFVFNRTKTGLTGSIEIITVRRRLTKA
                     TQKIQEITPQKSLLERESEEPSISTNTKPTIKPNTTHKQNVFFTKSEEKSETKQFKAV
                     ESHDFNNSEELMAFMAKINELIAQDEAMASPKDENESSSTVGEEYIEKLIEIMKNSEL
                     LKHEFWSRLDQMTYKDIQLFVSKLSPTKEKGKFGSSDLDYLWRYFNGFEFEPSKRENR
                     KDKFAVMLEALSEEQLKASFDSSDFLSLLNKDYYIETAANTLKRKQLALFASDSKRHD
                     ILNLMIKKLKPSAYLLGKLASVMPYATTKIKIALIDQIAHLPHSASFKIELAKLAEHH
                     IGMNTQTYIKRYQSLPALPSNTTHSKPPLSKWASVPAVSKPIYTNIPVAKEDWTDEAF
                     EAFLRELNATTYLINEKKIIDDLNSLPDHRIKMIIEKASSPDYKDMLKHFWMIESKTI
                     NHRSFIENRKNRLKIILENLSESQLKSSIKDNKFWDIFRNTQEPYCKMAAKVLSPRQF
                     EIIISNATLERHADFAVIITQIKKDSSADKERLQQIIEAIIPHTTPWFALALERQIKG
                     LLTSDKSLFEKFSTDLKKYLSKSLERPEVSLKYRRDRDLNRRAISHLLTEPLENSYKA
                     SFSL"
     gene            7985..8866
                     /locus_tag="I9760_001577"
     CDS             7985..8866
                     /locus_tag="I9760_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5043348.1"
                     /translation="MLKRVLNTAKAKQPISTTGIFAHTYYWLTSPAGDQSYQNPNYKN
                     EEDNNTGTAIYFIHGTADQPAAFKRVAERLIDTGLPDEICSLNLLAFDQRYQGKSIKF
                     FAEQLKNKIKVNQHQRVILMAHSRGGLVASYFAEFLAKEASIDVPLVITMGTPFNGSY
                     LAVKPLSWFSDSIREMEINSEFLAQLKQEIVEHSVSAYHFFIAKEDAIVPGESGYIKD
                     YVDKYPESLHILDRHGHLSIMSSHRLVSHISSLLHDYIDDYQNDTEEVKISKLGELTL
                     IEDYYPVKSEPDSLFNP"
     gene            9052..9471
                     /locus_tag="I9760_001578"
     CDS             9052..9471
                     /locus_tag="I9760_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAT5043349.1"
                     /translation="MNFSAWYFPSLAALILYGAWGYWGTRASDFINPLSITFYSSIGV
                     LISGIIALILLGFKPELSVKGSTYGLLNGLANGIACIFFILALRNGPTMPVVLVTSMY
                     PMITLIFCMIFLKQELSLKQGLGMVFALTALILFSTE"
     gene            9713..10987
                     /locus_tag="I9760_001579"
     CDS             9713..10987
                     /locus_tag="I9760_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1106 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043350.1"
                     /translation="MRNNKMGEKIPSFFHPDNKAASHQILVDDPLIIKDSELLDLLNA
                     SIEKDEKYHLLISCIRSHSPKYLNQRIMVKSHITIEGDERTPPHWTPLATKREYGYPT
                     DGDQNNELTTTLANIIATNELNFSSAQMVALFRELMIKGVDFTAMDQSPYLNLIHPKS
                     GNNPLKSLIAASLGDPEIIHLLHDFMSYIENIVPRDKQLLIINNQDNFEFEAMQPAEF
                     LLRLGHEDLALRLYRMGAQPTVQAFRLACSSYGTTIYYEEAMECIQFMMNSVELSETD
                     LVEGKNSLQKASPGQYNEMLTRILRSLQEDSKLDTGHSSLYEMIQKDYIENYQDKRFI
                     SFRDFCQKEAKIDHPLIQKYALLRIKQENVGNGYFSDYYRTESRHLSENKWAFLNKNQ
                     RASQLLINLINAIDLGLIDMELGSLSITSAKL"
     gene            complement(11086..11589)
                     /gene="msrB"
                     /locus_tag="I9760_001580"
     CDS             complement(11086..11589)
                     /gene="msrB"
                     /locus_tag="I9760_001580"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAT5043351.1"
                     /translation="MTKLISVVFYLFIIPNVMAYTPQFNKSEQLKKLTPLQYQVTQNG
                     ATEKSFDNAYWNNKEEGIYVDIVSGEPLFSSNDKYDSGTGWPSFTKPIDTSYLVFHTD
                     RSYFFIVRTEVRSKYANSHLGHVFKDGPEPTGLRYCMNSAALKFIPKKEMEQAGYGEY
                     LYLFNKK"
     gene            11788..12780
                     /locus_tag="I9760_001581"
     CDS             11788..12780
                     /locus_tag="I9760_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HAT5043352.1"
                     /translation="MNQKQIKNELALKWALEHLALNDEFKIIDQQKIIETAYSTVHRI
                     TSSESNVYYLKQTPEMLYLEPQTLTYIKEQRCHNIPQIIAENKHLNCFLMLSCGDETL
                     RNLFKENIDLEMLKAGILNYTKIQRLVENQLPQLMELGLPDWRLEKFSSLYDQLIQQE
                     ELLSSDGLSKNEITLLNKAHEICFNLCESLSKYQIPETINHCDFHENNMILNQKTGNV
                     SIIDWGETVITHPFFSLNGCLWNITFFYPIKQSDKIYEILQRQCITPWLGLHDETDLL
                     NALKIANQLNGVFAALSYKRIYDATMDQSQTVQQEHPGSIAGCLRSFINHFHLA"
     gene            complement(12824..14035)
                     /locus_tag="I9760_001582"
     CDS             complement(12824..14035)
                     /locus_tag="I9760_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5043353.1"
                     /translation="MVMKTMRLFKGILFTFCGIIFCFNSHAALEHEKLVNYIALGEFS
                     RETAEIALKKMPPLDTLTVHYDLQLYKINYKTQSPDGNLTIASGLVAMPIHPVGQVGI
                     ISYQHGTRFERNDVPSRNNEKNYIYLAAYGNSAGYMTVMPDYLGLGDNELTLHPYVQA
                     ETLASSSIDMLFAAKELANRLHYPISDKLYLAGYSEGGFSTIVMFEMLAKEYPDLPVS
                     AVAPGSAPYGWEETMHFVMLEPGPRATAYLAYFFYSLQTYKSYWSGFDEIFAPPYNTL
                     IPELMDGYHAVDEILQALPQDPLLIFQPKFSNGIISKTDRNTEILKINFNHYDFKPTA
                     PLLLVGTKGDRDVPYAGAEMAYHSFRKYSDFVWIKSVSDALDHVQAHPFVLKEQVDFF
                     KQFERQEAMNK"
     gene            14328..15191
                     /locus_tag="I9760_001583"
     CDS             14328..15191
                     /locus_tag="I9760_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043354.1"
                     /translation="MVITMSREEEQAKKKLEEDKVQEDKFLRSTVADYIREFSSMLHA
                     CEQRARRKPADRTALSAEKDAHTLREFLDDLQKAQQEGHGDLPILRTPAEWAAIDQMR
                     QRCVQYQIDPMQNHKMKNEKTEKPTHDSQVSEENLRRLQILDRYKETPRSEEEEEKER
                     KRLINLATSGNNTFIAYEDLETIAKSGKPFFIPSNSKEDACLSCGNGRLYKGSLSEIR
                     MQLEFDLKKDPDNEQMRTGLRRIENEIFMKPNLASPKKEKDFTEAGITPQENTYTTPN
                     PFSIKPKKEPI"
     gene            complement(15473..>15603)
                     /locus_tag="I9760_001584"
     CDS             complement(15473..>15603)
                     /locus_tag="I9760_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043355.1"
                     /translation="TLSKAQGLFTKVTGIKTDSQRAVEEIFSKALKDLQSPKLAMR"
BASE COUNT         5424 a         2900 c         2629 g         4650 t
ORIGIN      
        1 aaattaaagg aaaaactttt aaaaatttca agtaagggta tcagttcagc gtttgaaggc
       61 attattatca ccgattcgga aggcaccatt cttgaagcga atcaagcttt tatgaaaatg
      121 accggatata cagaacagga agtcataggc caaaaacccg ccatttttca atcaagaaag
      181 caaaatgaga aattctacaa ggaactatgg aagatattgg ctgaaaaagg acagtggagt
      241 ggtgagcttt gggctaaaaa gaaaaataaa aagttatttc cggctttttt agccatctca
      301 tccgttaaaa attctgaagg ggacgtaata aactacattg gttattatca tgatctaaca
      361 ctaataaaaa aacaagaaca acaaattgag aattttaaat attatgatta tttaaccggt
      421 ttaccaaata aatttttact cacccaaaga cttcagatgc tcatcagcgg attgtctgag
      481 gaaaataaag aactcatcgt ttttaatatt cgagtatttg actcaaacag caaaaactct
      541 ctgctggatt ttcctcccaa tattcgcgat aaaatcatat tggaagtggt taaccgtatt
      601 gatcgaacat cacctaaaaa aatgttttac tcacgcattg gtttaggtga atttattgtt
      661 atttatttta tcaaaagcag aattgaagtg atggcttcca ttgcgcaaag aatgatagaa
      721 aaactcgctt gtccttatta cattgatcac caaactatta ttttgcaaac gatcattgga
      781 ttaaccgcct attacaaaaa tagcgcattc agtgccgagg acttgctaca acaatcagaa
      841 attgcccgcc ataaagctca aatcaacggt gttaatattt ttgaattttt tgatccgcag
      901 gttgaagagc gaattataga atttaataaa cgcattgaat cagtctctaa agcaatgcag
      961 ctaaatgaac tgaaagttta ttaccaacct attgtagaat taaatacggc taaagtggtg
     1021 ggtttggaag cgctacttcg ttggcagcat ccagagaaag gtttattgct tcccaaacaa
     1081 tttttatccg acctcaataa tcatcctctt tctctacaga ttggtgattg ggtgcttcat
     1141 caggtattac atcaagttga agaattgctc gcttgcaata taaaaatccc catcaatgtt
     1201 aatgttagtg cttatcaact gcaacatccc aactttgtca aaaaactgtt tgacacatta
     1261 gcacgatacc ctaatgttcc tcatcgcctg cttgtgcttg aaatccttga aacagaagct
     1321 attgaagatt taaccctagt atcccagata atagccgagt gcaaggctaa aggaatcaac
     1381 ttttccttgg atgactttgg aacaggttat gcttctttaa ctttcctaaa agaattgagt
     1441 acctcggaag tgaaaatcga tcgaagtttt atcattaaca tctttgataa ccctaaagat
     1501 ctcgctattt tgaaaataat tatagagtct tgcaaagtga ttggccgcac cctgattgct
     1561 gaaggagttg aaacagtagc acatgggaaa ctattagaat tacttggttg ccatctaatc
     1621 cagggatacg ccattgccaa acctatccct cctgcaaaat taattatatg gataaaaaac
     1681 tggcatcttg gcccagaatg gagagaaacc agattttcac aagcagaact atctaatctg
     1741 gtaaaaatct ccataaaaca ctatctacat taccaatcaa tcagacttta tttagagaat
     1801 aatcaatccc ctaaacctga attatcactt gaagtatgtc caatagaaca gtggcttatt
     1861 gaaaatcaac aaacaatcaa agaaagcaaa cgatttaaaa aagtgtgtga tttgcatcac
     1921 aaagaacatg aggtgggaaa tacaataatt cagctaattc ggacagggca aaaagaacaa
     1981 gtggacaaac aactggcaaa gcttgatggc ttaagagata agttcttgaa agagttaatg
     2041 aatttaattt tttaattcaa ttactatttg aaagtgttgt agaatgaatt tcaataaaaa
     2101 atatttttta ttcaatgagt tataaattaa aatgtaggtg ggtatcacta atgacaagga
     2161 gatggtcgtg cgcaatctaa aattttctca tcccttttct attgggattt ctttattttt
     2221 attcattttg ttctttgttc ccttgctctc ttatggcgcc aagacatcca atatgcgtgg
     2281 caaaagatat tgtgaaatca tcctatcaaa aaccatctca agttatgctg tttataatac
     2341 ttggggacta aatgattgcc cagaacaatt atggagtaaa gtttctatgc ctgcagtaaa
     2401 aaaggaaaca ggggcttctt ttgtccatct caatggtcct cgatattggg taattgacgg
     2461 attcaaaaac acttctttga taaatcctgc tatcaaaaca attagtggaa ttccaatgcg
     2521 ggaagcaggt attttacatc ttagtttaat agatttattt aaaaataaac cttatcaatc
     2581 tcatatcgtg gaccgtaaaa caacatggat ctatcaagaa ggaaaacctg tctttgaatt
     2641 gattgatccc actgggcaag tttttgtcat gcaatcctat agtgttcaaa aatatccgca
     2701 aataatggat aacctcaaac aactaggtga taaacttcaa cttcctaaag gttggaaatt
     2761 taaaactggt gtgcttaaca agctagaaac aatagaagct gtcaataaca aagccgtagt
     2821 tgtccaggat aattttttga atacctatca gaaagccagc catgatttcc tgaaataaat
     2881 tcagctgata attggttact ttcataggtc atgagtttag tatgcgttga caatccatcc
     2941 tctactttgg gggtaatggc attaatgaac gtgttaatgg caatttcaac catctataaa
     3001 acctatgatt agcaaaccaa ggtaaaataa caaacatttt acaaatgttg tattataatt
     3061 aacctatatg gagtatcata gaggttaatt ataatgatat taagactaat ccttacttct
     3121 tatttgatta tcttccccta taagttttta atggctaatc aagttatgaa tactgctatt
     3181 aaggtattag aggaatgtta tgataaaacc actgatctta gaaaatatgt cccatgtgtt
     3241 gagacagaag cagataaaat acccgcctta caaaatttgc aaatacgcat aaaatttaaa
     3301 aatcctgaaa aaaattctaa agaaaaagta cccatattga tggtagataa aacaggatac
     3361 atgtattatt gtattgcaac agcaggaaaa agtttaacca tagattcttg cgctgggaca
     3421 cagggaaaac ctttgtctga ggggcaattg atgtcaatag agttactaaa agattaatat
     3481 ctgttattca aatgagttta atgcgattga catagataaa ctgtaaaatc ttgccttagt
     3541 taacaggttt ccaaaaaact cgattaatac ttattttcga tactcaaaga ataattaata
     3601 gtctttcaaa aacattcatt ttgcacataa ctttttctcc tggtttcaag caattattgt
     3661 tttcacatga tcaaataatg aatcaaaagt attgcaaaag ccaatttgat cctataagtt
     3721 actattgagt ttaaataaca gctaaaggac tattattatg ccaacttatt ttgatcctat
     3781 tatgcaagaa gatactgtac tggatgaaaa cacgatcgtt tacttagtta agattggtga
     3841 taataaattc agtattaaag caatttcatc aggattggag cacctcccca gtgatccaac
     3901 tactcacgcg gagaaatatt ggcctattcc tgccaaatca ttgattgatc attcaagtaa
     3961 caagctcctt tttgaagagg ataagttaac gaatcaaccc ataagcaaag atcaggtaat
     4021 agaactattt gcagttgatc ccgataagac tgaacccaag caatttagtg actccgttaa
     4081 gagagagtta actgaaaact gggcacgaga ggtactacag gatcaatttg ctcatgcagg
     4141 gcaaaaccct catttctttt tttatcaacc cctattcatc catgggcatc atggagataa
     4201 ccctgctcaa caacaggata atgcaatacc aattcctctc ccactcctga tgttttcctt
     4261 gataccaccc atgaaataat aataaccaac aacaaatgac attattattt tttaacatac
     4321 tcctggtacc tgagaatcaa ggccatgaac ctgagcagga aaatacaggc gagcatactc
     4381 atggacactc acattaaacc caacctgtat tactaactgt tgtaaatgtc gggcgcctcg
     4441 cccgacatct ccagtttgac tggaacgctc aacaccaatc cctttttaat aagatagctc
     4501 gcaaggtatt tgatggccta tacctatttt attatctaaa atgttaagtc cgattttgtt
     4561 ttgatcttct agttgtacaa aatattcttt taatgtttgg agagtgagtt catcagggta
     4621 gctgaacttt ttattactta ataatcgatt tagccatgaa gctttcatat ttagtgacga
     4681 cccgtaaaac ggtctgagat ccgtatgggc aagcatataa atctcaccta tccatggatc
     4741 aaggagataa ttttcagaga taactacagt atgattaatg tcttcaatat cataaataag
     4801 cgcaggtata ttttgttgtc tcagcaatgc agcaagataa aaagcctggt gttcacaatt
     4861 tcctgccatc ccctttgaca tggtattttt tattgccgcc ctcccatcca gaaaatcaat
     4921 cttgttgtcc tcaagatgtt taaatatcct gagcaggaaa actcttatct caccgatacc
     4981 atgaatagcg atagagctat tttcctcata ttgagtcatt ctatctttat tgatttttgc
     5041 gcttagttca ttaataacta atgcagaagt gctggcgatg cacactgaca tagttgtttt
     5101 cagagcatta catagttttt tgtaatactc acctgctgaa tctttgattt caatttccct
     5161 gtctttgaaa aatctgacca tgtctaatcc cttatatcca ttaagcgctt tagtttacat
     5221 acaagtatgg aatgagcaaa cagaacagcc tcttttcacc aaaaccactc ttgctaattg
     5281 tatttgctat acttcaattg gcacatataa aaatggagtt taaaatgaca aaatatagag
     5341 atgcaggaac cggccgatat gtcaccaaag agtttgcaaa aaaacatcct ggaataacca
     5401 ttggtgaaaa tagcaagcca aaatcacaaa gaaaacctaa aaaataatca ccattaagta
     5461 attggtttga ggctgcattt tatacaaaaa aaagaccatg ttacctttaa aatagctctc
     5521 taaaataggc cattgagaag tttgcttgat gttttagtat taatctatca atatacatgt
     5581 ggtgtacgag gtcaattcta ttaacgaatt ctcagtatag ttcagttaat agaataagcc
     5641 attactttta cattaaattt aaccatacgg atattagtta atataatgaa atatttttta
     5701 aaactgtctc gccaacattc ctcaaatcaa aaaaaaagaa cccagcctgc tcaatttgaa
     5761 gatcaccatt taagaattcg tcaacctcaa tatgccccta tcactattga cttggttggt
     5821 agcgtaaggt ttatctttac cttttcgtta ttttgtttta gagcgattgc tggtttactt
     5881 actggtgcga tttctcgaaa aaatcttttt gattttgtat ttaatagaac gaaaactggc
     5941 cttacaggtt caattgaaat cattacagtc agaagaagat taacaaaagc gacccagaag
     6001 atacaagaaa taactcccca aaaatcatta ttagaaagag aatcagaaga accgtctatt
     6061 tcaaccaaca cgaaaccaac tatcaaaccc aatactactc ataagcagaa tgtatttttc
     6121 acaaaatctg aagagaaatc agaaacaaaa caatttaaag ctgttgaatc ccatgatttc
     6181 aacaattctg aagaacttat ggcttttatg gcaaaaatca atgagctcat agcacaagac
     6241 gaggcaatgg ctagcccaaa agatgagaat gagagctcat ctacggtagg ggaagaatat
     6301 attgaaaaat taattgagat aatgaaaaac tcagaattat taaaacatga attttggagt
     6361 agactggacc agatgactta taaagacatt caattatttg tcagtaaact tagtccaact
     6421 aaagaaaaag ggaaatttgg ctcaagtgac ctggattatt tatggcgtta ttttaatggt
     6481 tttgaatttg agccttcaaa aagagaaaat agaaaagata agtttgcagt gatgcttgaa
     6541 gccctttcag aagaacagtt aaaggcttct tttgattcgt ctgatttttt aagcctttta
     6601 aataaagact actacattga gactgcagct aacacattaa aacgtaaaca attggcattg
     6661 tttgcttcag atagcaaaag acatgacatt ctgaatttaa tgataaaaaa actgaaacca
     6721 agcgcttatt tgttaggcaa acttgcttct gttatgcctt atgccactac aaaaattaaa
     6781 atagccctga tcgatcagat tgcccattta cctcattccg cttcttttaa gattgaactt
     6841 gcgaaattag ctgaacacca tattggtatg aatactcaaa cctatataaa acgttaccag
     6901 tccctacctg ccctcccatc caatacaacc cacagcaaac cacccctttc aaaatgggct
     6961 tctgtacctg ctgtctccaa accaatatat accaatatcc ctgtagcaaa agaagattgg
     7021 accgatgaag cctttgaggc attcctgcgt gaactgaatg caacaaccta cctcatcaat
     7081 gagaaaaaaa taattgatga tttaaacagc ttaccagatc acagaattaa aatgattata
     7141 gaaaaagcct cttcacccga ttataaggat atgctgaaac acttttggat gattgaatcc
     7201 aagacaataa atcatagatc tttcatagag aacagaaaaa atcgcctgaa aattatttta
     7261 gaaaatttat cggaatctca attaaaaagt tccataaaag acaacaagtt ttgggatatt
     7321 tttagaaaca ctcaagaacc ttattgtaaa atggctgcca aagtattatc ccccagacaa
     7381 tttgaaataa tcatttctaa tgccactctc gaaagacatg ccgactttgc agtaatcatt
     7441 actcaaatta agaaagacag cagcgctgac aaagaaagac tgcaacaaat tatagaagca
     7501 atcattcccc acactactcc atggtttgcc ttagctttag aacgccaaat aaaaggttta
     7561 ttgacaagtg ataaatcctt gttcgagaaa tttagcactg atttaaaaaa atacctcagt
     7621 aaatcgctgg aaagacccga agttagttta aaatacagaa gagacagaga tctaaatcga
     7681 cgtgctattt ctcatctcct gacagaacca cttgaaaact cttacaaagc gtcctttagt
     7741 ctttaaagat actctactac cttaagagct ctctgctttt gaccagtaag gcaccaatta
     7801 caaaaaacca atagagacgt taaccaaatc gcaattcatt cataattcat tgacaataac
     7861 tgcccccgat aactctttaa agcacagttt ataattgact catattaagc tatattattg
     7921 agaaattatt gacagtatcc gactttcgga actcgacgct ccacaataaa aaaggaattt
     7981 aactatgttg aagcgcgttc ttaatactgc aaaagccaaa caacccattt ccaccacggg
     8041 tatatttgct catacttatt attggttaac ttctcctgct ggcgatcaat cctatcaaaa
     8101 tcctaattac aaaaacgaag aagacaacaa cacagggacg gcaatctatt ttatccatgg
     8161 tacagctgat caaccagcgg catttaagcg tgttgctgaa cgtttgatcg ataccggtct
     8221 tcctgatgaa atttgctctc tgaatttact agcctttgat cagcgttacc agggaaaaag
     8281 tatcaaattt tttgctgaac aactcaaaaa taaaataaaa gttaatcagc atcagcgagt
     8341 cattttaatg gcgcactcca gaggagggct agttgcttct tattttgcag agtttttagc
     8401 aaaagaagcc tccattgatg taccgttagt aattacaatg ggcaccccgt ttaatggttc
     8461 ttaccttgct gtaaaaccat tgtcatggtt ttctgattct attagagaaa tggagattaa
     8521 tagtgaattt cttgcacaac ttaaacaaga gatagtagaa cactccgtta gtgcgtacca
     8581 tttttttatt gcaaaagaag atgcgattgt acctggagaa tcagggtata taaaagacta
     8641 cgttgataag tacccagagt cgctacatat tttagatcgc cacggtcacc tttccatcat
     8701 gtcttctcat cgtttggtat cacatatttc cagtttactc catgattata ttgatgatta
     8761 tcaaaatgat acagaagaag taaaaatttc taaattagga gagctcactc taattgaaga
     8821 ttattatccc gtaaaatctg aaccagattc ccttttcaat ccataaacgt ccattcatgg
     8881 gttttgtggt tattatttat tcagaaaaat aaactacatc agtaatgagc gtttttataa
     8941 accacagctc atcatgataa taaataaaat ttagtaacaa acagtattat tttcactaat
     9001 gaacagttat cctgagcaaa cctcaaacaa attgcttgat aagataaaat tatgaatttt
     9061 agcgcttggt actttccatc tttggccgca ttaattctgt atggcgcttg ggggtattgg
     9121 ggaacaagag cctcagattt cattaatccc ctgtctatta ctttttattc aagtatagga
     9181 gttttaattt ccgggattat tgctttgatt ttattgggtt tcaagcctga actttctgtc
     9241 aaagggagta cttacggttt gctaaatggt ttggctaatg gaattgcctg tatttttttt
     9301 attttggcat tacgtaatgg ccccaccatg ccggtagttt tggtcacctc tatgtatccc
     9361 atgattactt taatattctg catgattttt ctcaaacaag aattaagttt aaaacaagga
     9421 ttaggtatgg ttttcgcact gactgcctta attttatttt caactgaatg aaaacccaaa
     9481 tcagatgaaa tagcctacat ttctttgact tatcaaaatt aaagagattt acttaaaaac
     9541 tttggccaag caaaaatctt cttgactaat ataaacaatc taatcaagat aaaagcatca
     9601 tgcctaaaat aggaataaaa acacagttgt tgattgttac aaacaaacat gggttcgaag
     9661 cttttgttac attagctgtc gcttttaaat tttttgaaag aggttcttaa ctatgcgaaa
     9721 taataaaatg ggagaaaaga ttccaagttt ttttcatcct gataataagg ccgcctcaca
     9781 tcaaatattg gtagacgacc cgttaatcat taaagatagc gaattgttag acctgttaaa
     9841 cgcatcaatt gaaaaagacg agaaatatca cctcctaata tcctgcattc gcagtcacag
     9901 tcccaaatat ttaaatcaaa gaattatggt taaaagccat ataacaatcg aaggtgatga
     9961 gcgtaccccc cctcactgga cacctttagc aacaaaaaga gaatacggtt atccaaccga
    10021 tggggatcaa aataacgaat tgacaacaac actggcaaat atcattgcta ccaatgaatt
    10081 aaatttttct tctgctcaaa tggttgcctt gtttagagag ttaatgataa agggagttga
    10141 ttttaccgcc atggatcaat ccccctacct caatttaata catccaaaat ctggcaacaa
    10201 ccctctcaaa agcttgatag cagccagttt gggggatcct gaaattatcc acttactgca
    10261 tgacttcatg agttatattg aaaatatagt tcctcgcgat aaacaattat tgattatcaa
    10321 taatcaagat aattttgaat tcgaggcgat gcagccggct gaatttttat taaggctagg
    10381 ccatgaagat ttggctttac gcttatacag aatgggagct caaccgactg tacaagcatt
    10441 ccgactggct tgctccagtt atggcaccac tatttactac gaagaagcaa tggaatgcat
    10501 tcaattcatg atgaattctg ttgagttgag tgaaacggat ttagttgaag gaaaaaattc
    10561 cctgcaaaaa gcaagtccgg gacaatacaa cgagatgctg acaagaatat tacgttccct
    10621 gcaggaggat tcgaaacttg atactggaca ttcttctctc tatgaaatga tacagaaaga
    10681 ctacatagaa aattatcagg ataagaggtt catttcattt cgtgattttt gtcagaaaga
    10741 agcgaaaatt gatcatccgc tgattcaaaa atatgctctt ttacgcatca agcaagaaaa
    10801 cgtaggtaat ggttatttct cagattacta ccgcacagaa tccagacatt tgtctgagaa
    10861 taaatgggct ttcctcaata agaatcaaag agccagtcaa ttgctgataa atctcattaa
    10921 tgcaattgac cttggattaa ttgacatgga acttggatca ttaagtataa cctcggccaa
    10981 actataatct taggaacata aggcaagttc agctcatttc tattcctaca ttgatttgag
    11041 ctcaatgaac tccataggct aacttgctgg aaaaacaaag gcagtttatt ttttattgaa
    11101 cagataaagg tactctccat acccagcttg ttccatttct ttttttggaa taaatttcaa
    11161 ggcagctgaa ttcatgcaat atcgcaaccc ggttggttct ggcccgtctt taaagacatg
    11221 gcctaaatga gagttagcgt atttagagcg aacttcagta cgcacaataa aaaaatagct
    11281 tctgtcagta tggaaaacca aataggatgt atcgattggc tttgtaaaac tgggccaacc
    11341 cgttcctgaa tcgtatttat cattagaact aaacaaaggt tctcctgata ctatatcaac
    11401 ataaatacct tcctctttat tgttccagta tgcattatca aatgattttt ccgttgctcc
    11461 attttgggtt acctgatatt gcaatggagt cagcttctta agctgctctg atttgttaaa
    11521 ttgaggggta taggccatca cgtttggaat tataaacaga taaaatacaa cgctgattag
    11581 ttttgtcatg tcatactcca cattcactgt tcatgcccac attgataaca cttacttata
    11641 gtgtagcaag cgtaccccta ttatcagaaa tctacgtttc catcggaatt aatattcata
    11701 cccaaagcaa aagacaggca tgatgtttgg acttaaacta ttttatacat taaattcata
    11761 ttaaagatta ttagtcatga gcaaactgtg aatcaaaagc aaataaaaaa tgaactcgcc
    11821 ttaaaatggg ctcttgagca tctggcatta aacgatgaat ttaaaatcat tgatcaacag
    11881 aaaattattg aaaccgctta ttcaacagtg catagaatca cctcttccga aagcaacgtt
    11941 tattatctga agcaaacacc ggaaatgttg tacctggaac cccaaacatt aacctatatt
    12001 aaggaacaac gatgtcacaa cattccccaa atcattgcag aaaataaaca cttaaattgc
    12061 tttttgatgc tgtcttgtgg cgatgaaact ttacggaatc tgtttaaaga aaatattgat
    12121 ttggaaatgc ttaaagcagg tattcttaat tacactaaaa ttcaacggtt agtagagaat
    12181 caactcccac aacttatgga attgggtctt cctgattggc ggcttgaaaa attttcatca
    12241 ctatatgacc aactgataca acaagaagag cttttatcaa gtgatggact atcaaaaaat
    12301 gagattaccc tcctgaacaa agcacacgaa atttgcttta atttatgtga atcactctcc
    12361 aaataccaaa ttccagaaac aattaatcat tgtgattttc atgaaaataa catgattctc
    12421 aatcaaaaga cgggaaatgt aagcatcatt gactggggag aaaccgttat cactcatcca
    12481 tttttttctc tgaatggctg tttgtggaac ataactttct tttacccaat aaaacagtcc
    12541 gacaaaatat atgaaatatt gcaacggcaa tgtattactc cttggttggg cctgcatgac
    12601 gaaactgatt tattaaatgc gctaaaaatt gctaaccaac tcaatggggt ttttgctgca
    12661 ttaagttata agcgaattta tgacgcaact atggatcaat ctcaaacagt tcaacaagaa
    12721 caccctggtt ctatagccgg ctgcctaaga tcattcataa accattttca tttagcttga
    12781 ccgctacatc aaaactgtga ttgctgagtt tcataaaatg acattattta ttcattgctt
    12841 cttgacgctc aaattgttta aaaaaatcca cctgttcttt gagtacaaag ggatgagctt
    12901 gcacatgatc caatgcgtca ctgacggatt ttatccacac aaaatcacta tatttcctaa
    12961 aggaatgata tgccatttca gctcctgcat aaggtacatc acgatcacct ttagtgccaa
    13021 ccaataacaa aggagctgta ggtttaaaat catagtggtt aaaattaatt tttaaaatct
    13081 cggtatttct atctgtctta ctaataattc cattagagaa ttttggctga aaaataagca
    13141 gcggatcttg tggtaaggct tgaagaattt cgtcaacagc atgatatcca tccatcaatt
    13201 ccgggataag agtattgtaa ggaggagcaa aaatttcgtc aaaaccagac caataactct
    13261 tataagtttg cagagaataa aagaaatagg ccaaataggc tgtagcacga ggtcctggtt
    13321 ccaacatcac aaaatgcatg gtttcttccc agccataagg agctgaacct ggggcaaccg
    13381 cagagacagg taaatcagga tactcttttg ccagcatttc aaacattaca atagttgaga
    13441 atccgccttc tgaataacct gccagatata atttatcgct tattgggtaa tgaagcctgt
    13501 tggctaactc cttagcagca aacaacatat caatgctact gctggcaaga gtttctgcct
    13561 gaacataagg gtggagtgtt aattcattat ctcccagacc aagataatct ggcatcaccg
    13621 tcatataacc tgcactatta ccataggcag caaggtatat atagtttttt tcattatttc
    13681 tggaaggtac atcattgcgt tcaaatcggg tgccatgctg atagctaata attcccactt
    13741 gccccacagg atgaattggc attgcaacta atcctgaggc aatagtcaga tttccatctg
    13801 gtgactgggt tttataatta attttatata attgcagatc ataatgtact gtaagggtgt
    13861 ctaatggcgg catttttttt aaagcgattt ctgctgtttc cctggaaaat tcacccaaag
    13921 caatataatt aaccagcttc tcatgctcca gagcagcatg ggaattaaag caaaaaataa
    13981 tcccgcaaaa tgtaaaaaga atccctttga ataaacgcat cgttttcata accatctata
    14041 aaaatgtgga taattacatg tttaataagg agaatcaaat gaagcggtta cttgcattaa
    14101 tgcaaaggaa aaaatctgtc cctttcatca tctaacaaac aagtattcaa ttaaaacgcc
    14161 tgatgaaata aatgaagtat catctacaaa tgctcttgtt gctaaaaaat gcatgaattt
    14221 tattggaaaa ctcagagaga taagtaactc cagaaaaata tactatcatt aattttataa
    14281 gataagatta ttttcaattc cacagtgaat tgatgttcaa ataatttatg gtaatcacaa
    14341 tgtccagaga agaagagcaa gctaagaaaa aattagaaga agataaagta caggaagaca
    14401 aatttctcag aagtacagtg gctgattata tccgggaatt cagtagtatg ttacatgctt
    14461 gcgaacaacg agcaagaaga aaacctgccg acaggacagc actctcagcc gaaaaagacg
    14521 cacatactct tcgtgaattc ctcgatgacc ttcagaaagc tcaacaagaa ggacatggag
    14581 acctacctat actacgaacc ccggccgaat gggctgctat cgatcaaatg cgacaaaggt
    14641 gcgtacagta ccaaatcgat cccatgcaga accataaaat gaagaatgaa aaaacagaga
    14701 aaccaaccca tgactctcag gtttctgaag aaaatttacg ccgactacag atattagata
    14761 gatacaaaga aacacctcgc tctgaagagg aagaagagaa agaaagaaaa agacttataa
    14821 atcttgcaac cagcggtaat aatactttta ttgcttatga agatttggaa acgatagcca
    14881 agagtggtaa accttttttt atcccatcca actcaaaaga ggatgcttgt ctatcttgtg
    14941 gtaatgggcg tttgtataaa ggttcattat cagagattcg tatgcaatta gagtttgacc
    15001 taaaaaagga tccggataat gagcagatga gaactggttt gagaagaatt gaaaatgaaa
    15061 tttttatgaa acctaattta gcatcaccca aaaaagaaaa agactttact gaagcgggaa
    15121 taactccaca agaaaacact tacactactc caaacccatt ttcaataaaa ccgaaaaaag
    15181 aacctatata agcctatgta ctgatgagct taaccacttg gatccaaaca actggaaatc
    15241 ttgctaaagt gatgaagctt aatggttagt aacataagct ttctatcaac caataaaatg
    15301 ccgcaccgct gtcaagacaa acaaaacaca gttatttaaa caatgctggt agaacaggga
    15361 catggctatg agaacaactt gtactgctac agtcttcgcc aaattgaaaa atttaaattg
    15421 aatttgatga ggcagtcagc tgataaatgg caactgccca atcaaatttg agctacctca
    15481 ttgcaagctt gggtgattgt aaatctttga gtgccttgct aaaaatctct tctactgccc
    15541 gttgcgaatc agtttttatg ccagtaactt tagtaaataa cccttgggct ttgctaaggg
    15601 tca
//
LOCUS       DACTJE010000065        15525 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.065, whole genome shotgun sequence.
ACCESSION   DACTJE010000065 DACTJE010000000
VERSION     DACTJE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15525
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.065"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(45..1547)
                     /locus_tag="I9760_001585"
     CDS             complement(45..1547)
                     /locus_tag="I9760_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5043356.1"
                     /translation="MLNLFREQPRAFHMIFMLELWERFGFYTVQGILTLYFIRYLGYS
                     DLESYYTFGAYSALVYGMVVIGGYLGDRILGTKRTIVLGLVVLAMGYFSLAVTDKQHV
                     FLALGLICVGNGLFKANPSNLLSKCYDDNDPRLHGGFTLYYMAINLGSMVALFVGPAV
                     SSAYGYSYAYMISAIGLILGIVNYWYQRQHVSHVNTKADLRKITVFHWGIIIAGVLIL
                     TGISAYLLQHVMLAKNLLWGITLLVVSVYIYLMRKQAQASFMRMLVALVLMIEAVIFF
                     TLYQQMPTSLNLFAVNNVIPTFLGITIDPQSFQALNPIWIVLMSPVLAYFYRKLHQHN
                     ILFSIPYKFAFGMTMCAVSFLMLYFARYFYVGNGMVSSWWMIFSYLFQSLGELLVSAL
                     GVAMVAELVPATNAGFVMGMWFLTSAVAGFIGATVASFTALPENIKPGVESLLIYSDV
                     FACIGLVTLLIAVIMWLVSPRLSRYITVPSLEGDKDIETVEYSTTYIPSH"
     gene            complement(1615..2997)
                     /locus_tag="I9760_001586"
     CDS             complement(1615..2997)
                     /locus_tag="I9760_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043357.1"
                     /translation="MGITKDERFRKFEIEKTLQLLQSMQRPIEYPLTPIDKTSLLKKA
                     TKRIPLLSKFLKTVDDTGEVVVKIGDRMTSLSHTPGKESLAHGFAFGGVAMAGFDFLR
                     IPVIYLSAYLLGEEVPVNLSNNAKWFYSALLLGLTITALAVPVTAPYIAFASAGLSLG
                     VGTFLLGKTLYERYQLGRERRKLKAEILREEDEMLKIQDKAKHLESLLQSADNEEQIA
                     AILYEISVVQEDYSRQKERLQSLKNDELQLEQQIKKLGTMKVVDRTIAVALSALTIAA
                     LAVTLFFPPVGGGMLAGIAIFGGAYFLARVSMPLIKATAVWLYNKLKSTPSESSESQD
                     IDNKPTLSNEKEEKKEDSLQIDNEFVLPPIDAEASISEDSKEYQPVHESTEDVLVRLS
                     VNIDRLKEKVRMEKKEEIEEESPEELQHFSSLTPSPRTLGSRKLSAKESDDENGESES
                     DIEHPDVPKI"
     gene            3135..4247
                     /locus_tag="I9760_001587"
     CDS             3135..4247
                     /locus_tag="I9760_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HAT5043358.1"
                     /translation="MMVKKALYLAGGGARGAYQAGVLKAIGHILQTKTLPFDLVSGVS
                     VGSVNAAVLAENANDFPAALEKLEAIWGEIHCQQIYKASNYELSKSVMRNLSTLIIKQ
                     RQSGHLLDTTPLRQFLEESIDFTRIEQNIQEGYLDTFEVLSACYETHQTISFYAHNHS
                     EFEDWNYPRHSSQRAVINAQHILASSALPLFFPPVSLNGFHYGDGSIGLVAPLRGAIR
                     FQVDRILILGTRELPEFTDPETLRNGEIAFAHILGNMMNGLFLDNLDRDIEMVNRMNE
                     IATLLSMWKKRRSPWRPISTLHLRPSRAMASVAQSHYTSMPVLLRFLLNIMGARSHSG
                     DLLSFLLFEKEFTRELLELGYQDTITAATEVSAFFS"
     gene            complement(4297..5094)
                     /gene="aroE"
                     /locus_tag="I9760_001588"
     CDS             complement(4297..5094)
                     /gene="aroE"
                     /locus_tag="I9760_001588"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAT5043359.1"
                     /translation="MLRRFAVIGHPIAHSLSPVIHQMFAQQTQIELIYEKILGDDVKF
                     EQQISDFFIQYGNGLNVTLPYKKRAYELAKIRTQRCTLAGAANTLWMEENQLHADNTD
                     GIGLIRDLSRFLELKDKKILILGAGGAARGIIFPLLEAKPLQLIVANRTLEKAEELQR
                     QFPQINVTSFAELTEFFDLIINATSTSLSGQVIVLPEEVLSHKPFCYDLAYNQKTSTA
                     FVQYARNGGCEAVDGLGMLVEQAAEAFFTWNKVMPSTQKILEHLRSF"
     gene            complement(5304..7895)
                     /gene="pepN"
                     /locus_tag="I9760_001589"
     CDS             complement(5304..7895)
                     /gene="pepN"
                     /locus_tag="I9760_001589"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAT5043360.1"
                     /translation="MPDTTIYLKNYQPPSFTVETVELNFDLYDDHALVNSTLKLTRQN
                     DGALHLYGEELELIALHLNGRPLEHEKYVLTKDALIIEQCPDEIVLNIVTRIKPQENT
                     QLSGLYRSNHLFCTQCEAEGFRRITYYLDRPDVLATFTTRITADKKQYPILLSNGNLM
                     DTGETADGRHWVIWEDPFKKPSYLFALVAGNLACVRDSFVTCSGRIVDLRIYVEPGNE
                     DKCSHAMESLKKAMKWDEEVYGREYDLDIYMIVAVSDFNMGAMENKGLNIFNSKYILA
                     RPETATDQDFADVEGVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSRDMNS
                     RDVNRIMDVKVLRSAQFPEDAGSMAHPVRPESYQEINNFYTATVYNKGAEVIRMQHTL
                     LGKERFRRGMDLYFKRHDGYAVTIDDFVAAMEDANGVDLTQFKLWYSQAGTPEVRVLS
                     HFSEGRLEITMQQNCPPTPECHEKKPFHIPVRIALFDLKGEMIPIENELLELKEKEQR
                     FIFSGLNEKPVVSLLREFSAPVKIYDDLDQDDLLFLLRYETDGYAKWDAAQRLVLNCL
                     NENLKLPASEWQVSKTLVAAFQHVLLDDSLDMDLRAELLTPPGFEEVAATMKWVDVSK
                     VESVRDYFRQQLGLGLYTEASDLYKELWEVEDHRMHGQAYGRRKLRNVCLWLMMKANE
                     SDALELCREQFKASRTMTDQIASCSLLVNCSRESQRNEAIEGFYKQWSHNELVLDKWF
                     AMQAACELPGTLDHVKKLTQHPAFCIKNPNKVRALVGAFCMANPRNFHALDGSGYVFL
                     SEVLIKLDKLNPQIAARLATPFTRWRSYDEPRQKLIQNQLEQLTKLDLSRDLREVVDK
                     SLIVEEK"
     gene            8068..9999
                     /locus_tag="I9760_001590"
     CDS             8068..9999
                     /locus_tag="I9760_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HAT5043361.1"
                     /translation="MNTQDFLAGYTKRFVDNKEEDMSLDEYLELCKTDPTAYANPAER
                     LLMAIGEPELIDTRHDPVLSRIFSNKIIHHYEVFKDFYGMEEPIEQIVGFLKHAAQGL
                     EETKQVLYLLGPVGGGKSSIAEKLKDLMERIPFYAIKGSPVFESPLSLFNPLEDGELL
                     RERFGIPTRYLRYLMSPWAVKRLQEYNGDISKFRVIKVKPSRLKQIAIAKTEPGDENN
                     QDISSLVGKVDIRKLEEFSQDDPDAYSYSGGLCRANRGLLEFVEMFKAPIKVLHPLLT
                     ATQEGNYNATEGLSAIPFEGIILAHSNESEWQSFRNNKNNEAFIDRINIVKVPYCLRV
                     SEEIKIYQKLIHNSSLANAPCAPGTLDMLAQFSVLTRLKEPQNSSIYSKMRVYNGESL
                     KDTDPKAKSYQEYRDFAGVDEGMSGISTRFAFKILSKVFNFDHTEVAANPVHLMYVLE
                     RQIEQEQFPQELHETYLNFIKEYLATKYVDFIGKEIQTAYLESYSEYGQNIFDRYITY
                     ADFWIQDQDYRDPDTGEIFDRALLNLELEKIEKPAGISNPKDFRNEVVNFVLRARANN
                     RGKNPVWNSYEKLKSVIEKKMFTNTEDLLPVISFNAKASEEDKKKHEEFISRMVEKGY
                     TRKQVRLLCEWYLRVRKSQ"
     gene            10153..11418
                     /locus_tag="I9760_001591"
     CDS             10153..11418
                     /locus_tag="I9760_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HAT5043362.1"
                     /translation="MSQLIDRRQNAGKKSTVNRQRFLRRYKSQIKKAVSEAVGKRSIT
                     EIDQGEQITIPAKDIYEPQFHRGHGGHIERVLPGNDNFIAGDRIKRPGGGGAGGAGGN
                     ASDSGEGEDNFVFELSREEFLELYFEDLELPDLVKKELARISTYKTVRAGVTTSGIPN
                     NINVLRSMKQATGRRVALASPYKKRLKEAEEELERLKQLANPDKIDLLKLERDIEFFK
                     KKIQTVPFIDTIDLRYNHRVRVPSPSTQAVMFCVMDVSGSMDEAKKDIAKRFFILLYM
                     FLTKNYEKIELVFIRHHTSAKEVNEEEFFYSRETGGTVVSSALELLNTIIEARYPPQA
                     WNIYVAQASDGDNWNADSPYCQELLQEKIMPLLQYFAYIEIMPRHHQSLWEVYQQVKE
                     RYPNFAMENIDNVADIYPVFRELFKRKTV"
     gene            11415..12938
                     /locus_tag="I9760_001592"
     CDS             11415..12938
                     /locus_tag="I9760_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAT5043363.1"
                     /translation="MRKKPLSTGAEWTFELIQAYDREIARIAKSFNLDTYPNQIEIIT
                     AEQMMDAYSSVGMPIGYHHWSFGKHFVSVEKSYKRGQMGLAYELVINSNPCISYLMEE
                     NTMAMQALVIAHACYGHNSFFKNNYLFKMWTSADAIIDYLVFAKKYISDCEERYGIDA
                     VEPVLDACHALMNYGVDRYKHPTPLSIQEEKIRQQNREIYMQSQVNELWRTIPFSKQI
                     DKNGQKKRFPEEPQENILYFIEKNAPLLEPWQREIVRIVRKLAQYFYPQGQTKVMNEG
                     WACFWHYTILHALYDEGLVTDEFMLEILQSHTNVIMQPAYNSPYYNGINPYTLGYHMM
                     QDIKRICENPTDEDKKWFPYLANTDWLTSLDTAMRNYKDESFIAQYLSPKLIRDLKLF
                     HIVDDDRNSELYINAIHDEWGYQQIREALSRQYNLGYLEPDIQVYSVDLQGDRSLTLR
                     YSQQNRVPLGSSTNEVLKHLHYLWQFPVILQAVDGENKITEEFSCPPLNKNKTAETN"
     gene            13230..14090
                     /gene="vipE"
                     /locus_tag="I9760_001593"
     CDS             13230..14090
                     /gene="vipE"
                     /locus_tag="I9760_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VipE"
                     /protein_id="HAT5043364.1"
                     /translation="MPLTQTQRLINTYGASLKNGTISNEELIILLDPNTFTKSEGYVD
                     PNAPVSDSNHSKMDAIKDFVLTIGPTLDSEILHQLTSRMIELSPPGDRNTFMRGSSLE
                     KAFLAFEMAHYPTKAEEHFNSTRVRTEFPGENDIDNLKAVILNPIIAFFQSPPGTDEK
                     LEKLIKLCTDYKEHLKPSLTDDANNLVNKKHAIVTNMLAALTDSTAPNNATKIKNMEQ
                     ILTEENKATLKDRRDSFSGARFLESILHILSLGIYSKLTKGTFKFWKSHGEALSDNIE
                     EVSKQQPGMK"
     gene            complement(14171..15376)
                     /locus_tag="I9760_001594"
     CDS             complement(14171..15376)
                     /locus_tag="I9760_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAT5043365.1"
                     /translation="MRFNQWMTCITSGLILASNSSFATTSPVHEQLQVPQCLAAKITV
                     PHKILAENKEFKIIDVLSSDVETLTILADKVSCGHFVNVSHKLTGTLAANQQQSAQKL
                     LQKKLVKPFGVSKLHKDVYEIKHEEEVNAALKEIVSDNIWQTLTHMTSYYNRSATKDT
                     GVDTANWLKSKFEQMAVEYGRTDTSTFFVKTGWYKQPSLVTVIGKDIKAPAIVIGAHM
                     DTLDGRMPGAGDDGSGSSSIMEAARVILSSKTTFKRPIYFIWYAAEERGLVGSQHVVQ
                     HFQEQSIPVKAVVQFDMTGYRNDANDPTMWVFTDYTDRDLSNYLAKLIDHYIHVPVDY
                     SRCGYGCSDHASWNEEDIPAAFPCETSFADHNPYIHTSSDKMDLLNLEHMTNFSKLAV
                     AFAIELASE"
BASE COUNT         4996 a         3232 c         2853 g         4444 t
ORIGIN      
        1 gatattaaaa ccaactcaat tgccgaaaag ggaaataaac aaacctagtg actggggata
       61 taggtcgtag agtattctac tgtttctata tccttatccc cttccaagga agggactgta
      121 atatagcgac ttagccgagg tgatactaac cacataataa ctgcaatcaa tagagtaaca
      181 agaccaatac aggcaaatac atcggaataa atcaataaag actcaacccc tggtttgatg
      241 ttctctggaa gtgctgtaaa agacgcaaca gtagcgccaa tgaaccctgc cactgcggaa
      301 gttaaaaacc acatccccat aacaaaccca gcattagtag ccggcaccaa ttcagcaacc
      361 attgcaacac caagagctga tactaataac tcgccaagac tttggaataa ataactgaaa
      421 atcatccacc aggaagaaac catgccattt ccaacataaa aataacgagc aaaatacagc
      481 attaaaaagc tcactgcaca catggtcatg ccgaatgcaa atttataagg tatagaaaat
      541 aaaatattat gttgatgcaa ttttctatag aaataagcta atacgggact cattaataca
      601 atccatattg ggttgagagc ttgaaagctt tgaggatcaa tagtgatgcc caggaaagta
      661 ggtattacat tatttacagc gaacaaattt aatgaagtag gcatctgttg gtataaagta
      721 aaaaatatga ctgcttcaat cattaaaacc aaagcgacta acattctcat aaaagaagcc
      781 tgagcctgtt tgcgcattaa atagatataa acagacacta ccaagagagt aataccccac
      841 aacagatttt tggcaagcat gacatgttgt aataaataag ctgatattcc agtaagtatt
      901 aaaacacccg ctattattat tccccagtga aagactgtta ttttacgaag atccgctttt
      961 gtattgacat gagacacatg ttgtcgttga taccaataat tcacaatacc caaaatcaaa
     1021 cctattgcac taatcatgta agcataagag taaccataag cgcttgatac tgctggtccg
     1081 acgaataatg caaccatact gcccagatta atagccatat aataaagggt aaaaccacca
     1141 tgtaatcgtg gatcattgtc atcataacat ttggataaca aattcgatgg attcgctttg
     1201 aatagcccat tacctacaca aatcaatcct aatgcaagaa atacatgttg cttatcagta
     1261 actgcaagag aaaagtaacc catggccagc acaacaagac cgagaacgat ggttcgcttg
     1321 gttcctagaa ttctgtctcc caaatagccg ccaataacca ccattccata taccagcgca
     1381 gaataagcac caaaggtata gtaagactct aaatcgctat aacccaggta gcgaataaaa
     1441 taaagagtaa gaattccctg aactgtatag aacccaaatc gctcccataa ttccaacata
     1501 aaaatcatgt gaaatgcacg aggttgctca cgaaataaat tcagcatgaa ttttcattcc
     1561 ttgatagtaa ttttttttaa cttaaaggaa attcttgttt tttgcaatgc agatttaaat
     1621 tttagggaca tcaggatgtt ctatatctga ctcgctttct ccattctcat catcactctc
     1681 ttttgctgat agttttctgg atcccaatgt tcttggggag ggagttagag atgaaaaatg
     1741 ctgaagctcc tctggggact cttcctctat ttcctctttt ttttccattc ttaccttttc
     1801 cttgagcctg tcgatattta ctgataacct caccagtaca tcctcagttg attcatgcac
     1861 aggttgatac tcctttgaat cctcagaaat tgacgcctca gcatctattg gtggtagaac
     1921 aaactcgtta tcgatttgca aagaatcttc ttttttttct tctttctcat tacttaaagt
     1981 cggtttatta tctatatcct gtgattcaga actctcactt ggggtcgatt ttaatttatt
     2041 gtacagccag acagctgttg ctttaattaa aggcatgctt actctagcca agaaatacgc
     2101 tccgccaaaa atggcaatcc cagccaacat gcctcctcca accggcggga aaaataaagt
     2161 cacagctaaa gcggcgatag taagcgcaga caaagcaacc gcgatagtcc tgtccacaac
     2221 tttcattgta cccaattttt tgatttgttg ttctaattgc aactcgtcat tttttaaact
     2281 ctgaaggcgt tctttctgcc tgctatagtc ctcctggaca accgatattt catataaaat
     2341 ggctgctatt tgctcttcat tgtcagcgct ttgtaaaagg gattccaaat gctttgcctt
     2401 atcctgaatt ttcagcattt catcctcttc ccttaagatc tccgctttta attttcttct
     2461 ttcccgacct aattgatagc gttcataaag agtttttcct aataaaaacg tacctacacc
     2521 taaactgagg cctgctgaag caaaagctat ataaggagct gttaccggga ctgccaatgc
     2581 ggttatggtc aatcctaaca atagggcgga ataaaaccat ttggcattat tactcaaatt
     2641 aacagggact tcttcgccta gcaaataagc cgacaaataa atgacaggaa tcctaagaaa
     2701 atcaaagcct gccatggcaa caccaccaaa ggcaaaacca tgagccaaag actctttacc
     2761 aggtgtatgg gataaactgg tcatcctatc accaattttg acaactactt cacccgtatc
     2821 atcaacagtt tttaaaaatt ttgacagcaa aggaatgcgt tttgtggctt tttttaatag
     2881 tgacgtttta tctataggcg tcaacggata ttcaatagga cgttgcatcg actgtaagag
     2941 ttgaagagtc ttttctattt caaattttct aaatcgttcg tcttttgtaa ttcccatgat
     3001 aatgactcaa gaaaagaatc gatttaattt aagtatatca attcataatt aagaaaagat
     3061 gacatcgata attctttctt gctaattagc ccagatttca actatatttc aagttataat
     3121 aaataaaata aaacatgatg gttaaaaaag ctctatattt ggcaggtggt ggtgctcgag
     3181 gcgcttatca agcaggcgta ctcaaagcta tcggtcatat tttacaaacc aaaaccctac
     3241 cttttgactt agtgagtggc gtcagcgtag gcagcgtcaa tgcggccgta cttgctgaaa
     3301 atgccaatga ttttcctgct gccctggaga aactcgaggc tatttggggt gaaattcatt
     3361 gtcaacaaat ctacaaagcc agtaactacg aattaagcaa atcagtaatg cgtaatttaa
     3421 gcaccttgat tatcaaacaa cgtcaatcag ggcatttgct tgatacaaca ccattacggc
     3481 aatttctgga agaaagcatt gatttcacac gaattgaaca aaatatacaa gaaggatacc
     3541 tcgatacttt cgaagtttta agcgcctgtt atgaaacaca tcaaaccatc tctttctacg
     3601 ctcataatca ttcagaattt gaagattgga attatccccg tcatagcagt caacgtgccg
     3661 tgatcaacgc ccagcatatt ctggcatcaa gcgccttacc attgttcttt ccccctgtca
     3721 gcctcaatgg attccattat ggtgatggaa gcataggctt ggtcgccccc cttcgcggtg
     3781 ctattcgttt tcaagtggat agaattctca tcttaggtac cagggaattg cccgaattta
     3841 ctgatccaga gaccttgcgt aatggtgaaa ttgctttcgc tcatattcta ggcaatatga
     3901 tgaatgggct ttttctggat aatctggata gagacattga gatggtcaat cgtatgaatg
     3961 aaatagcgac actcttatct atgtggaaaa aacgacgctc tccctggaga ccaatctcaa
     4021 ccctgcattt acgcccaagc cgagcaatgg cctcagtagc ccaaagccat tacaccagca
     4081 tgccagtatt gcttcgattt ttattaaata ttatgggggc cagaagtcat tccggtgatc
     4141 tacttagctt tttactcttt gaaaaagaat ttactcggga attactggaa ttaggatacc
     4201 aggacaccat tactgcagcg acagaagtga gcgccttttt tagttaacca gagtttggtt
     4261 gccaaagtgc atctagttgt tatgcaagct tccaatttaa aaagatcgca ggtgttctaa
     4321 tattttttgg gtagaaggca taactttgtt ccatgtaaag aatgcttccg cagcttgctc
     4381 tactaacata cctaatccat caactgcttc gcaccctcca ttacgagcat attgaacaaa
     4441 ggcggtgctt gttttttgat tataagccaa atcataacaa aatggtttat gggataacac
     4501 ctcttcaggt aatacaatga cctgaccgga caaactcgta gaagtggcat tgatgattaa
     4561 atcaaagaat tctgttaatt cagcaaaact tgtgacattg atttgaggaa attgacgctg
     4621 taattcttct gctttttcca aagtccgatt agcaacaatt aattgtaacg gtttcgcttc
     4681 caataaagga aaaataatcc ctcttgcagc tccgccagcc cccagaatca gaattttttt
     4741 atcctttaac tcaagaaatc gggataaatc acgtatcaac ccaataccat cggtattatc
     4801 agcatgtaat tgattttcct ccatccatag ggtgtttgct gctccagcca gggtgcatct
     4861 ttgcgtgcgt atttttgcta attcataagc gcgcttttta taaggtaaag tgacattaag
     4921 cccgttgcca tactggataa aaaaatcaga gatttgttgt tcaaacttga catcatcacc
     4981 caagattttt tcataaatca attcaatttg ggtttgttgg gcaaacattt gatgaatgac
     5041 aggagacaaa ctatgtgcaa ttggatgacc tatcactgca aaacgacgaa gcacaaaaac
     5101 tcctttatcc tataggtttc attggttttc ctaattataa actgttctca cgcaacaagc
     5161 acatcaatcc aagttgttct taaatcgcac ccggtgatca agctgaaaag ttagttgatg
     5221 aattttgaaa gctttgtttg cacaaattcg tttaagtcgt gctgaatctg cctatagcaa
     5281 aatccagcac actttaattc atcctatttt tcctcaacaa tcagactctt atctacaacc
     5341 tcgcgtaaat cccgcgacaa atcgagtttt gtaagctgct ccaactggtt ctgtattaac
     5401 ttttgtcttg gttcatcata acttcgccaa cgcgtaaaag gcgtagccaa gcgtgcggcg
     5461 atttgaggat ttagtttgtc gagcttaatc aatacctcac ttaaaaagac atatccgctg
     5521 ccatccaacg catggaagtt tctggggtta gccatacaaa aagcgccaac cagcgcccga
     5581 actttattcg gatttttaat acaaaaagca ggatgttgag ttagtttttt gacatgatcc
     5641 aatgtgcctg gcaactcaca tgccgcttgc atcgcaaacc acttatccag taccaattca
     5701 ttatgagacc attgtttata aaatccttct atggcttcat ttcgttgaga ttccctggag
     5761 caattgacta gtaagctgca actggcaatt tgatccgtca tggtacgaga agccttgaat
     5821 tgttctcggc ataattctaa agcatctgat tcgttcgctt tcatcatcaa ccataaacaa
     5881 acatttctta attttctgcg tccataagct tggccatgca tgcgatgatc ttctacttcc
     5941 cataattctt tataaagatc agacgcctca gtgtataagc ccaatcccaa ttgttgacgg
     6001 aaataatctc gcactgattc aaccttgctg acatcaaccc atttcatggt agctgccact
     6061 tcttcaaatc ctggaggtgt caacaactct gccctgagat ccatatcaag agagtcatca
     6121 agcagaacat gctgaaaggc agcaacaaga gttttagaga cttgccattc actggctggc
     6181 aatttcaagt tttcattgag acagttaaga accaggcgtt gtgccgcatc ccacttggca
     6241 tatccatccg tttcataacg caataaaaat aacaagtcat cctgatccag atcatcataa
     6301 attttcacag gggctgagaa ttctcttaat agcgagacaa caggcttttc atttagccca
     6361 gaaaaaataa aacgttgctc cttttccttt aactccagaa gctcgttttc tatgggaatc
     6421 atttcccctt tcaaatcaaa taatgcaatg cgaaccggga tatgaaatgg ctttttctca
     6481 tggcactcag gcgtaggagg acaattttgt tgcatggtaa tttccaatcg tccttctgaa
     6541 aaatggctga gaacgcgcac ttcaggtgtc cctgcctggc tataccagag tttgaattgc
     6601 gttaagtcaa ctccattggc atcttccatg gcagcaacaa aatcatcaat ggttacggca
     6661 tagccgtcat ggcgtttgaa ataaagatcc atgcctcgtc gaaacctctc cttgcctaac
     6721 aaagtatgtt gcatgcgaat cacttccgcg cctttgttat atactgtcgc cgtgtaaaaa
     6781 ttattaattt cctgatagga ttccggcctt actggatgcg ccatgctccc ggcatcctcg
     6841 ggaaactgag cactgcgcaa taccttaacg tccataatcc gattaacatc acgagaattc
     6901 atatcccgag aaaactcctg atcccggaat acagtcaatc cctcttttaa acttaactgg
     6961 aaccaatcgc gacaagtcac acgatttcct gtccagttat ggaaatactc atggcctact
     7021 acaccttcaa catccgcgaa atcttgatca gtggcggttt cagggcgagc gaggatgtat
     7081 ttggaattaa atatattcaa ccctttgttt tccattgctc ccatattgaa atcactcacc
     7141 gcaactatca tataaatatc taaatcgtac tcacggccat aaacctcttc atcccatttc
     7201 atggcttttt ttaatgattc catggcatga gagcacttgt cctcattccc cggttctaca
     7261 taaatccgta aatcaacaat cctgccggag caagtaacaa aactatctct aacgcaagct
     7321 aaattgcccg ctaccaaagc gaataaataa gagggcttct tgaaaggatc ctcccaaatc
     7381 acccaatgac gtccatccgc ggtttctccg gtatccatta aattaccgtt agacaataaa
     7441 atcggatatt gctttttatc tgcagtaatt cgtgtagtaa aagttgccaa aacatcgggt
     7501 ctatcaagat aataggtaat tcgtctgaaa ccttctgctt cgcattgagt acagaataaa
     7561 tggttagaac gataaagtcc tgataattgc gtattttctt gaggtttaat gcgagtcaca
     7621 atattaagta ctatttcatc cgggcattgc tctataataa gagcatcttt cgtcaacaca
     7681 tacttctcat gttctaaagg tcgcccattt aaatgcaaag caattaattc cagctcttcg
     7741 ccatacaaat gcaaagcgcc atcgttttga cgtgttaact taagagtgct gttcactaga
     7801 gcatggtcat catacaaatc aaaattcaat tccacagtct caactgtaaa agaaggcggt
     7861 tgataattct tcaaataaat ggtcgtgtct ggcatagtca ttgcttccta taaaaaaaac
     7921 gaataagttt attgatttat gtattaaatt aagtggttag tctactatac tatgaattaa
     7981 aagaagtaat ttttgctttt atacaggaaa gagaaaaaaa agaatcacat tatatccttc
     8041 tcagggttaa gtaaaagggg atggcgcatg aatacacaag attttttagc gggttatacc
     8101 aaacgatttg ttgataacaa agaagaagac atgagtttgg atgaatatct ggagttgtgc
     8161 aaaacggacc cgacagcata tgccaaccca gcagaacgtt tgctcatggc gataggcgag
     8221 cctgaattga tcgatacccg acatgatccg gttctatcac gaattttctc aaataaaata
     8281 atccatcatt atgaagtttt taaagatttt tatggaatgg aagaacccat tgagcaaatc
     8341 gttggctttt tgaaacatgc tgcgcaaggg ttagaggaaa caaaacaggt tttatatctt
     8401 ttggggcctg ttggcggtgg taaatcttcc attgcagaaa aattgaaaga tttaatggaa
     8461 agaatccctt tttatgcaat caaaggttct cctgtctttg aatctccact gtcattattt
     8521 aaccccttgg aagatggtga attacttaga gaacgttttg gtatcccaac acgctaccta
     8581 cgctacttaa tgtccccctg ggcagtcaag cgattacagg aatacaatgg cgacatcagc
     8641 aaattccggg ttattaaagt caagccttct cgactcaaac aaattgccat tgcaaaaaca
     8701 gaacctggcg atgaaaacaa tcaagacatt tcttcactgg ttggtaaagt cgatatccgt
     8761 aaattggaag aattttccca agatgatcct gatgcttaca gctactctgg aggcttgtgt
     8821 cgagctaacc gcggactttt ggagttcgtt gaaatgttta aggcaccgat aaaggtcctt
     8881 catcccttgc tgaccgcaac gcaagaaggg aattacaatg ccacagaagg gctttcagct
     8941 attccttttg aaggaattat actagcccac tccaatgaat cagaatggca atcatttaga
     9001 aacaataaaa ataacgaggc atttatagac cgaatcaaca tagtcaaagt gccttattgc
     9061 ctgcgtgttt ctgaagaaat caaaatctat caaaaactaa tccataacag ttctttagca
     9121 aatgcccctt gcgcacctgg tacactggat atgctagccc agttttctgt attaacccgt
     9181 ttaaaagaac cacaaaactc cagtatttac tccaaaatgc gcgtgtataa tggagagagt
     9241 ttgaaagata ccgatcccaa ggcaaaatcg tatcaggagt atagagattt tgcaggggtt
     9301 gacgaaggga tgagcggaat atccacgcgg tttgccttca aaatcctttc aaaagtattc
     9361 aattttgatc atactgaagt ggccgccaac ccggttcatt taatgtatgt gctggaacgc
     9421 caaatcgaac aggaacaatt tcctcaagag ttacatgaaa cctacttgaa cttcattaaa
     9481 gaatatttgg cgaccaaata cgtcgatttt ataggcaagg aaattcaaac cgcttacctg
     9541 gaatcctatt ctgagtatgg gcagaacata tttgaccgtt acattaccta tgctgatttc
     9601 tggattcaag atcaggatta tcgcgatccg gatactggcg agatatttga cagagcctta
     9661 ttaaacctgg aactggagaa aatcgaaaaa ccggcaggga tctccaatcc taaagacttc
     9721 cgtaatgaag tagtcaattt cgtactgcgc gctcgagcaa ataaccgagg aaaaaaccct
     9781 gtatggaata gctatgaaaa actaaaatca gtgattgaga aaaaaatgtt taccaatacg
     9841 gaagatttgt tacccgttat ttctttcaat gccaaagcat ctgaggaaga taagaaaaaa
     9901 cacgaagagt ttatctctcg aatggtagaa aaaggctaca cacgaaaaca agttcgctta
     9961 ctttgtgaat ggtatttgag agtaaggaag tcacaataat aggtaggatc attcataact
    10021 caataggtta tgaaatacag tcagggttaa taagtaacta tctgaaaaga tcaagtagaa
    10081 tcctaaattc agatactgct tacaacctgg ctaacaaaaa actgacctgc agaaggtgtg
    10141 gagctaacaa ttatgtcgca attaattgat agacgacaaa atgctggaaa aaaaagcacg
    10201 gttaatcgcc aacgtttttt acgccgttat aaaagccaaa ttaaaaaagc agtctctgag
    10261 gctgtcggaa aacggagtat tactgaaatt gaccaaggcg aacaaatcac tataccagcc
    10321 aaagatattt atgagcccca attccataga ggccatggtg gccatatcga acgtgtacta
    10381 cctggaaatg ataacttcat cgccggagac aggataaaaa gacccggcgg tggaggtgct
    10441 ggaggagctg gaggtaacgc cagcgatagt ggtgaaggcg aagataattt tgtttttgaa
    10501 ttatctcgag aagagttttt ggaactctat tttgaagatc tggaattacc cgatttggta
    10561 aaaaaagaac tagcaagaat tagcacttat aaaacagtaa gggctggcgt aaccactagt
    10621 ggaataccta acaacattaa tgtccttcgc tcaatgaaac aagcgacagg acgccgtgtc
    10681 gctttagcct ccccctataa aaagagatta aaggaagcag aagaagagct tgaacggctg
    10741 aaacaattgg ccaatccaga caaaatagac ctattgaagc ttgaaagaga cattgagttt
    10801 ttcaaaaaaa agatacaaac tgtaccattt atagatacaa tcgatctgcg ttataatcac
    10861 cgtgtgcgtg tgccttcacc atctacacaa gcagtgatgt tttgcgtaat ggacgtatca
    10921 ggttccatgg atgaagcgaa aaaagacata gcaaaacgct ttttcatcct actctatatg
    10981 tttcttacca aaaattacga aaaaattgaa ttggttttta tccgccatca cacctcagcc
    11041 aaagaagtta atgaagagga atttttttat tcgcgcgaga ctggcgggac tgttgtttcc
    11101 agtgccctgg agttattaaa caccatcatc gaggcacgct accctcctca agcctggaat
    11161 atttatgttg cccaagcatc agatggtgat aattggaatg ccgactctcc ttattgtcag
    11221 gagttgctgc aagaaaaaat tatgccttta ttgcaatatt ttgcctatat tgaaattatg
    11281 ccccgtcatc atcaaagttt gtgggaagta tatcaacaag taaaagaacg ttatcctaat
    11341 tttgccatgg aaaatattga taatgttgct gatatttatc cggtatttcg cgaattattt
    11401 aaaaggaaaa cggtatgaga aaaaagcctc tatcaactgg tgcagaatgg actttcgagt
    11461 taatacaggc ctatgatcgc gaaattgcac gcatcgccaa aagctttaat ttggatacat
    11521 atccaaacca aattgaaatc attaccgctg aacaaatgat ggatgcctat tcttcggtgg
    11581 gtatgcctat cggttaccac cattggtctt ttggaaaaca ttttgtcagt gttgagaaaa
    11641 gctacaaaag aggacaaatg ggcctggcct atgagcttgt aattaattcc aatccctgta
    11701 tttcttattt aatggaagaa aataccatgg ctatgcaagc cttggttatc gctcacgcat
    11761 gctatggcca caattctttt tttaagaaca attacttatt taaaatgtgg acttctgcgg
    11821 atgctattat tgactatctg gtattcgcca agaaatacat cagcgattgt gaggaacgct
    11881 acggaataga tgcggtagaa ccagtgcttg atgcttgcca tgcccttatg aattacgggg
    11941 ttgatcggta caaacatccc acacccttat ctatccagga agaaaaaatt cgacagcaaa
    12001 accgtgaaat ctacatgcaa tcacaagtta atgagctatg gagaacgata cctttcagta
    12061 agcaaatcga taaaaacgga caaaaaaaac gctttccaga agagcctcag gaaaatattc
    12121 tctattttat agaaaaaaat gcccctttat tagagccttg gcagagagaa atagtgcgta
    12181 tagtaagaaa actggcccaa tatttttatc ctcagggaca aactaaagtg atgaatgaag
    12241 ggtgggcctg cttttggcat tatactattt tgcatgcttt gtatgacgaa ggtttagtga
    12301 ctgatgaatt tatgctggaa atactgcaaa gtcataccaa tgtcatcatg caaccagcct
    12361 ataatagccc ttattacaat ggtattaatc catacaccct tggctaccat atgatgcaag
    12421 acatcaaacg aatttgtgaa aacccaacag acgaagacaa aaagtggttt ccctatctcg
    12481 cgaatactga ttggctcaca agccttgata cggcgatgcg caactacaaa gatgaaagtt
    12541 ttatcgctca atacctgtca ccaaaactta tacgagattt aaaactgttc catattgttg
    12601 atgacgaccg aaactccgaa ttatacatca atgccattca cgacgaatgg ggatatcagc
    12661 aaatcagaga agcattatcc agacaataca atctgggtta tctggaacct gatatccaag
    12721 tctactccgt tgatttgcaa ggagatcgtt ctttgacttt aaggtactcc caacaaaaca
    12781 gagtaccact tggaagctca acaaatgaag tattaaagca cttgcattat ttatggcaat
    12841 ttccagtcat attacaggca gttgatgggg aaaataaaat caccgaggaa tttagttgcc
    12901 cacctcttaa taaaaataaa acagcggaaa ctaattgata gtcttatatt actggctcaa
    12961 aatagctatt agttttctat tataactaag ggccagtatc tattcttttt cttctcctta
    13021 attcactgcc cttaatatgc tgccaaccac ccgatctaat gactgctcct gatgcgatct
    13081 tgaagcaacg aaacaatttt atctggctgc tccttagaca atacctttca ttgaggtaat
    13141 gcgggttgtg gcaatagaaa tactctccta ataatttctt aataattttg tactataatt
    13201 tgccataacc aatcattgga gtcactgcaa tgcctttaac tcaaacccaa cgcctgatta
    13261 atacctacgg cgcttcactt aaaaatggaa caatctctaa cgaagaactg attattttgc
    13321 tggatccaaa cacttttaca aaatcagaag gttatgttga tcccaatgcc ccggtttcag
    13381 actccaatca tagtaaaatg gatgcaataa aggattttgt attaaccatc ggcccgactt
    13441 tagattctga aatacttcac caattaacat ctcgaatgat cgaattatcg ccccccggag
    13501 atagaaacac atttatgaga ggctctagct tggaaaaagc tttcttagct tttgaaatgg
    13561 ctcactaccc aacaaaagca gaggagcatt tcaactcgac aagagtgaga acagaattcc
    13621 caggcgaaaa tgatatagac aatcttaaag cagttatttt gaacccaatc attgcatttt
    13681 tccaatcgcc tcccggaacc gatgaaaaat tagaaaaact tatcaaatta tgcacggatt
    13741 ataaagagca cttgaaacct tctctaaccg acgacgctaa taatttagta aataaaaaac
    13801 atgcgattgt tacaaacatg ctagccgccc tcactgacag cacagcccca aataacgcaa
    13861 caaaaattaa aaatatggag caaatactga ctgaagaaaa caaagcaacc ttaaaagatc
    13921 gccgagattc atttagcggt gctagatttc tagaaagtat cctgcacata ttgagtctgg
    13981 gtatttattc aaaactgacc aaaggcactt ttaaattttg gaaaagccat ggcgaggctc
    14041 tctccgataa cattgaagaa gttagtaaac agcagcctgg catgaaataa atatcaatcg
    14101 atatcaataa attaacttta aaaaatagcc tctcaaaaat atttaaaatt tatgagaggc
    14161 attaaaacag ttactcggaa gcgagttcaa tagcgaaagc taccgcgagt ttggagaaat
    14221 tggtcatatg ttccaggttc aataaatcca ttttatcaga agaagtatgg atgtatgggt
    14281 tgtgatcagc gaaactggtt tcacatggga atgcagcagg aatatcttct tcattccagg
    14341 aagcatgatc actgcaacca taaccacatc gtgaataatc tactggcaca tgaatataat
    14401 gatcaattaa tttggccaaa taattactta aatctctatc tgtgtaatca gtaaataccc
    14461 acatagttgg atcattggca tcatttcgat aaccggtcat gtcaaattgt accacagcct
    14521 tcactggaat tgattgttcc tgaaagtgtt ggactacgtg ttgtgaaccg accaaacctc
    14581 tttcttcagc agcataccaa ataaaataaa tggggcgctt aaatgttgtt ttagaagaca
    14641 aaataacccg tgcggcttcc ataatgctcg atgatccact tccatcatca ccagccccag
    14701 gcatacggcc atcaagagta tccatatgag caccaataac aatagcgggg gctttgatgt
    14761 ctttgccaat cacggtaacc agggaaggtt gtttatacca ccctgttttt acaaaaaagg
    14821 tcgaggtatc agttcgtcca tactcaacgg ccatctgttc aaatttggat tttaaccaat
    14881 tggccgtatc aacacccgta tcttttgttg cagagcggtt ataataggaa gtcatatgag
    14941 tcagagtttg ccatatgtta tcagagacaa tttcttttag tgccgcattg acttcttctt
    15001 catgcttgat ttcataaacg tctttatgta atttggatac accaaagggc ttaacgagct
    15061 ttttttgcag taacttttga gcagattgct gttggtttgc cgctaaggtt cccgttaatt
    15121 tatgactcac attcacaaaa tggccgcaac tgactttatc agccagaata gtcaaggtct
    15181 ctacatcaga tgaaagtaca tcaataattt tgaattcttt attctcggcc aaaatcttat
    15241 ggggtacggt tatttttgca gcaaggcatt gtgggacttg caattgctca tgaacaggcg
    15301 acgtcgttgc aaaacttgaa ttactggcga gtattaatcc tgatgtgata caggtcatcc
    15361 attggttaaa acgcatatcc tctcctaagt tatttaaatc gtttttgata ctacctgatg
    15421 aaacgaatat tgcaaacaaa ctttattgtt gaattgaaac aaagacaaaa aatacttttt
    15481 taatattaat gctattgata atcattttca tttggtataa agttc
//
LOCUS       DACTJE010000066        15369 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.066, whole genome shotgun sequence.
ACCESSION   DACTJE010000066 DACTJE010000000
VERSION     DACTJE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15369
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.066"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1632)
                     /locus_tag="I9760_001595"
     CDS             complement(<1..1632)
                     /locus_tag="I9760_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAT5043366.1"
                     /translation="MIAELGLFFLILALVASLMLALIPLAGLQFNRKDWIDSSRNYVV
                     LQFLFVACAYLCLTACFLMDDFSVNYVLNNSSLLLPWFYKFCAVWGGHEGSMLLWVAI
                     LSFWMILVCFFSSSLDTEMRARVLVILGWLSIGFILFLLMTSNPFLRQFQVLNTQGRD
                     LNPLLQDPGFLFHPPMLYMGYVGFCVAFAFAIAALWAGKVETSWSKWTRPWTLAAWCC
                     LTAGITLGSWWAYRELGWGGWWFWDPVENASFMPWLVGTALLHSLAVTEQRQQFKAWT
                     MLLAIAAFSLSLIGTFLVRSGVLTSVHAFAVDPQRGLYILGFLLFVIGGSLTLFLFRA
                     QTLQVNKHPGLLSRESALLLNNVFLVVIMLTVLMGTVYPLLVEGLGLGKLSVGAPYFN
                     AVFVPLMIPLLLLMGLGVRLKWQNDCWRDVFRKLNRIFLLSLILPVILLFCTSTEIHL
                     TALLGLILAFWVLLSTANAAYHKVRDRGMAGVGQAYWGMVLAHCGVAATVIGIAVSSA
                     YGVQDDVRMSPGKQVNLLDYQIGFINQTPLSGPNYK"
     gene            complement(1629..2060)
                     /gene="ccmE"
                     /locus_tag="I9760_001596"
     CDS             complement(1629..2060)
                     /gene="ccmE"
                     /locus_tag="I9760_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAT5043367.1"
                     /translation="MTPVRRRKLFILLFALSVLSAAAALVLYALRQNISLFYTPTQIV
                     AGEAPAKHHIRVGGMVEANSIVRAKKGLDVQFKITDFENTIVVTYSGILPDLFREGQG
                     IVAEGEVTDNHHFHATQVLAKHDANYMPPQVKSALADKVKQ"
     gene            complement(2066..2224)
                     /gene="ccmD"
                     /locus_tag="I9760_001597"
     CDS             complement(2066..2224)
                     /gene="ccmD"
                     /locus_tag="I9760_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAT5043368.1"
                     /translation="MNQFFEWLAMGGYSFYVWPAYGLVCVVLGMNILGIKWQRTRTRK
                     KLQRWFKR"
     gene            complement(2208..2963)
                     /gene="ccsA"
                     /locus_tag="I9760_001598"
     CDS             complement(2208..2963)
                     /gene="ccsA"
                     /locus_tag="I9760_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HAT5043369.1"
                     /translation="MWKILYQLASPKNFYNYAGRLIPWLAVSALATIVIGMVWGLVFA
                     PPDYQQGDAYRIIFVHVPSAFLSMALYAWMGFLAILLLVWRIKMAGLLIHKVAQLGAW
                     MAFLALITGSIWGKPMWGAWWVWDARLTSELILLLLYLAILATYQAVKNKDDGDKIIA
                     ILALVGLIDLPIIHYSVYWWNTLHQGATLSVFSKPKIALSMLYPLLITLLGFFLYSLW
                     IILEKARNEVLFRERKQSWVKIQFEEQSDESVF"
     gene            complement(3177..3857)
                     /gene="ccmB"
                     /locus_tag="I9760_001599"
     CDS             complement(3177..3857)
                     /gene="ccmB"
                     /locus_tag="I9760_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAT5043370.1"
                     /translation="MNSPFQLFGKQFRREILIQVRQIRYLVNSCLFFLMLLFIFPLTL
                     RPELMLMRMFAPGLVWMAILLSMLLSLERLFQQDYEQGVLEQWLVSGQPLNLLVTAKV
                     MAHWLISVTPLLLLSPLIAILFSFNVWETCILLLSLICGTPAILFLCALAAAFGIGIN
                     QRGALMALILLPLTLPLLIFGSGTLSVAMQGLPVSGYLALLLAMSLLAVGFLPYAIAA
                     VIRISHID"
     gene            complement(3854..4456)
                     /gene="ccmA"
                     /locus_tag="I9760_001600"
     CDS             complement(3854..4456)
                     /gene="ccmA"
                     /locus_tag="I9760_001600"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027225196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HAT5043371.1"
                     /translation="MLDVIELDFDYHDQPLLQQISFHLPAGGLLHLKGSNGAGKTTLL
                     KLIAGLLNPEKGEIFFERQSIKKDLCTYQKQLCFVGHRSGINPYLTLRENCLYDIHFS
                     PGAVGITELCRLFSLEHLIDYPCGLLSSGQKRQVALLRLWMSKAKLWLLDEPLVALDE
                     LSLLTIITKIQEHRAKGGAVLLTSHQDLPLNKADYEEYHL"
     gene            4604..5539
                     /gene="sohB"
                     /locus_tag="I9760_001601"
     CDS             4604..5539
                     /gene="sohB"
                     /locus_tag="I9760_001601"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAT5043372.1"
                     /translation="MEFLSEYGMFLLKCITLVIALLILLAGIFSMGRKTKPKLEITSL
                     NEEYEHLNALMNKEILGKKPGKKKKDKTKRPVLYVIDFSGDIKATQVEQLRDEVTSIL
                     SIAKPEDEVLVRLESPGGAVNGYGLAASQLQRIRDKKIPLTVSIDKMAASGGYLMACV
                     ANKIIAAPFAIIGSIGVVAQIPNFHRWLKKNNIDVELLTAGEYKRTLTLFAENTEKGR
                     KKFQEDLEKIHTAFREYVLKNRSQLDIDKVATGEHWIAKDAFDLRLVDKLATSDEYLI
                     EKMAEFNAFKLTVHAKLPIISKVLKPAMRLIHPWI"
     gene            5763..6044
                     /locus_tag="I9760_001602"
     CDS             5763..6044
                     /locus_tag="I9760_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043373.1"
                     /translation="MSDFKSKLPDLNELTSMTAKLFKGIKTSVSEIIQDYKQKRAQPV
                     STEEKTTENKPAESKAEPKTESKPAENKTESKAESKPESNTEETQEKEK"
     gene            complement(6086..7501)
                     /locus_tag="I9760_001603"
     CDS             complement(6086..7501)
                     /locus_tag="I9760_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAT5043374.1"
                     /translation="MRSNDRIYIIDDDKERCDKLRTIFDFIGQSSEITQYGNWQLSVN
                     PNPSVIVLGASTCFEKMMDELDTLVKRFPKTPILVIDAVLSTSECADRNIVDCLSFPF
                     SYAKMLKSLHRCQIAKEAVKFITAGTHKTPLFRSLVGNSEGIRQVRKLIEQVADTEAS
                     VLILGESGTGKEVVARNIHSLSSRANKPFVPINCGAIPGELLESELFGHEKGAFTGAI
                     TSRQGRFELANGGTLFLDEIGDMPLAMQVKLLRVLQERCFERVGSNKSIEVNVRIIAA
                     THRNLEIAIQEGKFREDLFYRLNVFPIEMPPLRNRSSDLSLLFNELVSRIEGEGRPTV
                     RLMPDAMDALCEYSWPGNVRELANLVERLSILYPNGIIGKSDLPHRFQGEYKPSCSEA
                     SGAERETLLQIIKQESIANAEGIDLKEHLVKTELALISQALDESDWVVAHAASYLNMR
                     RTTLVEKMRKYGLTRPERMER"
     gene            complement(7696..8289)
                     /locus_tag="I9760_001604"
     CDS             complement(7696..8289)
                     /locus_tag="I9760_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043375.1"
                     /translation="MSNFRNFLVFILLFFSFCGQLTASSKQNLAFRNFWHPTYLGQRL
                     DYCTLDGKECGKEVANRYCQMLGYDYSTQNVIAYNVGLTNYLGSKAQCKGWRCNGFMT
                     IACAIGLSHTPPKPYHYREKKFAVPRYNDYRVDWCMERNKACGKQAANSFCNRMGYMQ
                     AKDFVKQAQVSATKTIGSQELCFGNQCNAFKMIICYR"
     gene            8679..9695
                     /locus_tag="I9760_001605"
     CDS             8679..9695
                     /locus_tag="I9760_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAT5043376.1"
                     /translation="MKKYLTFIVSGLALIAIIWFIRHLWIIGTVKVVRPVYGTAVQAV
                     YATGTVEPTIMMPIASQNTARLVKLNADEGSEVVKGQILASLEDEHLQHNLKELKEHE
                     KLAKIRYERNAQLVKKGVISKDEYDRTLSEWQAASAAVHRIEAQISYLQLIAPADGRI
                     IKRDGEVGQLIPANQPVFWLSCCAPLRVSAEVDEEDIVLVRVGQKVLIRSDAFPNQIF
                     YGRVKSITPKGDPIARSYRVRISFSKNNPLLIGMTAETNIIVHEKKNALLLPVSSISE
                     NKVWIVEDDGKLSQNTVTIGARGLKQAEILKGVNINDLVVLQPDNKLKNGQKVRFILV
                     KQEE"
     gene            9695..10951
                     /locus_tag="I9760_001606"
     CDS             9695..10951
                     /locus_tag="I9760_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT5043377.1"
                     /translation="MKLFLKIALKHLLARKRQSLVSLMGIVLGVAFFLTISSLMQGSE
                     KDFIKRLVDNSPHITIIDEYRNPRIQPIQQLHTHGAIEIRSVKPLTEPRGIRGYEQTI
                     NNLSLTIPGIIISPVLIGQALISYAGKDYSVSLNGMIPEEIKKVSTIENYMTSGSIDD
                     LIVNPDGIVLGSELLRKLSLTKDDNITVTAPNGQIRTFKILGVFRTGRSDFDTNQAFI
                     SIKRAQALLNRPNRSNNIIIKLPNPYQAYDIAAQIEKQIGYKTISWQEKSEDLLNTLV
                     IRNIIMYSVVSAVLIVAAFGIYNVISTVVMEKHRDIAILKSIGFQKHDIQFIFIIEGF
                     LLGLVGCLLGLPLGSGLMYSLMQVQFKPPGSSELINMPLDWSYLQFVIATAFAMSASI
                     IAAVLPAHKAALVHPVDILRGGGGGM"
     gene            10948..11625
                     /locus_tag="I9760_001607"
     CDS             10948..11625
                     /locus_tag="I9760_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5043378.1"
                     /translation="MNKIVIKTKELSRQLSGEVPVTLVKDINLEVREGEFLVISGPSG
                     SGKSSLLYLLGLLDSPSQGTIWLNNEEVSTYEEEKMAWLRLTQIGYVFQFHFLLPEFT
                     ALENVMLPMQRLGKLSLGEMKNRATKLLTSLGLEDQLSKLPKQLSGGQSQRVAIARAL
                     ANDPILLLADEPTGNLDTVSSTNVQNILKELAHEYRRSVIIVTHDMNFAATADRVIHI
                     VDGKMQE"
     gene            complement(11811..12569)
                     /locus_tag="I9760_001608"
     CDS             complement(11811..12569)
                     /locus_tag="I9760_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAT5043379.1"
                     /translation="MITRDELSLFLLDFLNCSQYQDYAPNGIQVEGKDKIKRICTAVS
                     ASEDVISQAIEQQADALLVHHGYFWKGENPVISGMKRRRIAKLLGHNMNLFAYHLPLD
                     CHPELGNNASLANLLLIESPEMHKVNNTANLLWSGKLSKAMNSEQFSSFLEHKLGRYP
                     VHIAGNEKMIHSIAWCTGAAQDFIEEAYQLGVDAYLSGEVSERTFYQAMELGIQYFSC
                     GHHATERFGIQSLGVYLANYFDLEHWFIDSPNPI"
     gene            complement(12566..13834)
                     /gene="murA"
                     /locus_tag="I9760_001609"
     CDS             complement(12566..13834)
                     /gene="murA"
                     /locus_tag="I9760_001609"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAT5043380.1"
                     /translation="MDKLLINGGKALHGEVVISGAKNAALPIMAASLLASDHVTISNV
                     PHLKDITTMMELLGQLGAHLIVDEKMNVQVDSSQVNEFVAPYDLVKTMRASILVLGPM
                     LARFGKADVSLPGGCAIGTRPVDLHLKALRAMGADITVKNGYINARCKKGRLQGKRLM
                     FDTVTVTGTENVLMAAVLAEGITTIKNAAREPEVVDLANFLIQMGAKIRGAGTSTIEV
                     EGVESLNGGTYSVMSDRIEAGTYLAAGALTRGQVTVKKVRPDTLLSQLCKFEEAGAEL
                     TIGEDWVSLNMHNKRPQAVNIATAPYPAFATDMQAQFMAMNSVAEGSSTIIETIFENR
                     FMHVQELQRMGANIQLNGNTAIVHGVEKLTGAPVMATDLRASASLILAGLVAEGETVV
                     ERIYHVDRGYERIEEKLSLLGADIKRVSDR"
     gene            complement(13827..14072)
                     /locus_tag="I9760_001610"
     CDS             complement(13827..14072)
                     /locus_tag="I9760_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HAT5043381.1"
                     /translation="MLSNEEIEQRLNEIDDVFYVKVEGDGYQYQVTVVSDIFLKKSKV
                     ARQQWVYAQLKDFITTGRLHAISMKTWTKEEWGKQNG"
     gene            complement(14462..14743)
                     /locus_tag="I9760_001611"
     CDS             complement(14462..14743)
                     /locus_tag="I9760_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HAT5043382.1"
                     /translation="MEINNFKPGAELTFKSVVSVRDKLYKALMEDTRERFCLDLSEVT
                     HCDSAGLALIIEARKLCKQNNKVFDVIGISPETQSLAEFCGVKTILQRS"
     gene            complement(14746..15354)
                     /locus_tag="I9760_001612"
     CDS             complement(14746..15354)
                     /locus_tag="I9760_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT5043383.1"
                     /translation="MRVFKTILLVVLMTLSQAMVAQTSPIPMLERAANQIISILKENK
                     ARLKSNPEIIYKAVEENLLPNVDVAGMSRSVLGRQAWSKASEAERVEFSKAFTRLVIR
                     TYSSPLAQYSDETVQFLPLRGSLNSRFIRVNSVIVRSAGQNIPLSYSLVSKNGKWKIY
                     DISVEGVSLLQSFRSQFAQALQHSSISQVIKEMQQKQIKKAS"
BASE COUNT         5105 a         3271 c         2704 g         4289 t
ORIGIN      
        1 tttgtaattt gggccactca acggagtctg attaatgaat ccaatctgat aatccagcag
       61 attcacttgc ttgccaggag acatacgtac atcatcttgt acgccataag ctgaagaaac
      121 agcaatgcca atcactgttg ctgcgacacc acaatgagcc aaaaccatcc cccaataagc
      181 ctgtccaact cctgccatcc ctctatccct gactttgtga tacgccgcat tagcagtact
      241 caacaaaacc cagaatgcca aaattaatcc caaaagggct gtaaggtgta tttcagttga
      301 ggtacagaat agtaaaatga caggcaatat cagactaagc aaaaaaatac gatttagctt
      361 tctgaagaca tcccgccaac aatcattctg ccatttcaaa cggacaccta accccattag
      421 caacaataaa ggaatcatta atggaacaaa tactgcatta aaatagggcg ctccaacgga
      481 taatttacct aaccctaacc cttccaccag caatggataa accgtgccca ttaataccgt
      541 cagcataata accactaaaa acacattgtt taataacaac gcactttctc gggataataa
      601 gccgggatgt ttattgactt gcaaggtttg ggctctgaaa agaaacaatg ttaaagaacc
      661 cccaatgacg aataacaaaa atcccagaat atataatccc cgctgaggat caaccgcaaa
      721 ggcatgaact gaagttaaaa ctccagagcg aacaagaaaa gtaccaatta aacttaaaga
      781 aaaagcagcg attgcaagca acatggtcca cgctttaaat tgctggcgtt gctctgtaac
      841 tgccaaagaa tgcaatagtg ccgtaccaac cagccagggc ataaaagaag cgttttctac
      901 aggatcccaa aaccaccaac caccccagcc taattctcta taagcccacc aactgcctaa
      961 agtaattccc gcggttaaac aacaccaggc agccagagtc cagggtctgg tccatttaga
     1021 ccaactggtt tctactttcc ctgcccataa ggcggcaata gcaaaagcaa aagcgacaca
     1081 aaaccctaca tatcccatgt acaacatagg aggatggaat aaaaagccag gatcttgcag
     1141 caatggattc agatcacggc cctgtgtatt caatacttga aattggcgca agaaaggatt
     1201 ggaagtcatt aacaaaaaaa gaataaaacc aatgcttagc caacctaaaa taactaatac
     1261 ccgagcgcgc atttccgtat caaggcttga gctaaaaaaa caaaccagga tcatccaaaa
     1321 actaaggata gcgacccata acagcatgga gccttcatgc ccaccccaca cagcacaaaa
     1381 tttataaaac catgggagca ataagctgga gttattcagc acatagttca ccgaaaaatc
     1441 atccatcaaa aaacaggccg tgagacatag gtaagcacat gccacaaata aaaattgcag
     1501 tacgacataa ttcctggagg aatcaatcca gtctttccga ttgaattgca aacccgccaa
     1561 tggaatcaaa gctaacatca aagaagccac taaagccaaa atcaaaaaaa acagccctaa
     1621 ttccgctatc attgtttcac cttatcagct aaagcagact ttacttgagg tggcatataa
     1681 ttggcatcgt gttttgccaa aacttgtgta gcatgaaaat gatgattatc agttacttct
     1741 ccttccgcaa ctattccttg cccttctcga aataaatcgg gaagtatccc tgaataggta
     1801 acaacaatcg tgttttcaaa atccgtaatt ttaaactgga catccaaacc ttttttggca
     1861 cgtactatgc tgtttgcttc caccatacct ccaactcgga tgtggtgttt tgctggtgct
     1921 tctcccgcaa caatctgcgt gggggtgtaa aataaactga tattttgtct taaggcatat
     1981 agaaccaagg cagccgctgc agataaaaca gacagggcaa acagtaagat aaataatttg
     2041 cgtcgccgta caggggtcat ttaacctatc tcttaaacca acgttgtaat tttttgcgcg
     2101 tacgagttct ttgccacttt attcctaaaa tattcatgcc caaaaccacg caaaccaaac
     2161 cataagcagg ccaaacatag aaagaataac cgcccattgc caaccattca aaaaactgat
     2221 tcatcagact gttcctcaaa ttgaatctta acccatgatt gctttctctc cctgaataag
     2281 acttcattac gtgctttttc caaaatgatc cataaggaat acaagaaaaa acccagcaaa
     2341 gtgattaaca aaggatacaa catactgaga gcaattttgg gtttggaaaa cacagataaa
     2401 gtcgcacctt ggtgtaaagt attccaccaa taaactgaat aatgaattat aggtaaatca
     2461 attaagccca ccaaagctaa aattgctatt attttatctc catcatcttt attttttacc
     2521 gcttgatagg tagccagaat tgccagatag agtaacaaaa gtattaactc tgaggtcagg
     2581 cgcgcatccc atacccacca ggcaccccac atgggtttac cccaaatgct ccctgtaatt
     2641 aaagcaagaa atgccatcca ggcacctaat tgcgcgactt tatgaatcaa aagccctgcc
     2701 attttgatac gccacaccaa caataaaatg gccagaaacc ccatccaggc atacaatgcc
     2761 attgataaaa aagcgctagg cacatgaaca aaaataattc ggtatgcatc cccttgctga
     2821 taatctggtg gagcaaatac caatccccaa accataccaa tgactatggt agctaaagca
     2881 ctgactgcca accagggaat gaggcgtccc gcgtagttat aaaaattttt tggcgatgcc
     2941 aactgataca atatcttcca cataggaaac aataaaatca aaaagaagga ttctaccacg
     3001 catcaaaaca atcacaaaat ttagttccaa acttagggtc tgttgacatt tcgctcgctt
     3061 gagtcgaaaa gccttgattt tcgagcaccg tagcggagca tacatagaag tatgtgagta
     3121 acggagcaca gaaaatcaag gcttttcggc caagatagta gaaatgtcaa tagaccctag
     3181 tctatatgac taatacgtat cactgccgca atcgcatagg gtagaaaacc aactgccaat
     3241 agagacatcg ccaataataa agccagatag ccgctaaccg gcaaaccttg catcgcaacg
     3301 ctcaaagtac cactaccaaa tatcagtaaa ggtaaagtta acggcaatag aatcaatgcc
     3361 atcaaagccc cacgctgatt tatccctata ccaaaagctg ctgcaagcgc acataaaaat
     3421 aatatggcag gcgtcccgca aataaggctc aataacaata tacatgtttc ccaaacattg
     3481 aatgaaaata atattgcaat caaaggagat aataataata gcggtgtcac agatatcaac
     3541 cagtgtgcca taactttggc tgtcacgagg agatttaatg gttgccctga aactagccac
     3601 tgttcaagaa ccccttgttc ataatcttgt tgaaacaacc gttctaacga aagaagcatg
     3661 gataacaaaa tggccatcca cactaatccg ggagcaaaca tacgcatcaa catcaattct
     3721 ggtcttagag taagaggaaa aataaataac aacattaaaa aaaataaaca cgaattaact
     3781 aaatatctga tttgccgcac ttggattaat atttcacgcc tgaactgttt tccgaataat
     3841 tgaaatgggg aattcataga tgatactcct cataatctgc cttgtttaaa ggcaaatcct
     3901 gatgagaggt caataataca gcaccacctt ttgccctgtg ctcctggatt tttgtgataa
     3961 ttgtaagcaa tgataactca tcaagagcaa ccaaaggttc atcaagcaac cataattttg
     4021 cttttgacat ccataatctt aataaagcaa cttgcctttt ttgtcctgag gatagtaatc
     4081 cacagggata atcaataagg tgttctaaac tgaataatct gcataattct gtaatcccca
     4141 ctgcaccagg agaaaaatgt atgtcatata aacaattttc tcttagtgta agatagggat
     4201 taatccctga tctatgcccc acaaaacata attgcttttg ataggtgcat agatcttttt
     4261 ttatagattg cctttcgaag aaaatttctc ccttttcagg attaagcaaa cctgcgatta
     4321 atttaagtaa agtcgttttt cctgctccat tggaaccttt taaatgcaat aagcctccag
     4381 caggaagatg aaaagaaatt tgttgcaaca atggctggtc atgatagtca aaatcaagtt
     4441 caatcacatc aagcataaaa cactttattt ttagatacac tcggctaact ataacaggac
     4501 ataagaaaaa tccccaatgc cttcgtgaaa agttatttaa tttttgcttg cgaagtgagt
     4561 ggatgatcgc cataatctgc tttatttttt gcaaaggtat atgatggaat tccttagtga
     4621 atatggcatg tttctcttaa aatgcatcac tctcgtcatc gcattactca ttcttctggc
     4681 aggtatattt tctatgggtc gcaaaaccaa acccaaactg gaaattacct ctcttaatga
     4741 agagtatgag cacctgaatg ccttaatgaa taaagagatt ttaggaaaaa aacctggaaa
     4801 aaagaaaaaa gataaaacaa agcgcccggt tctttatgtc attgatttca gcggtgatat
     4861 caaagcaacc caagttgaac aactaagaga tgaagtcaca tcgatattaa gtatagcaaa
     4921 acctgaggat gaggttttag taaggttgga aagccctgga ggggccgtca atggttatgg
     4981 acttgctgcg tcccagttac agcgaatccg tgataaaaaa attcctttga ctgtcagcat
     5041 agacaaaatg gcagccagcg gcggatattt aatggcctgc gtagcaaata aaataattgc
     5101 tgccccattt gcaataatcg gctctatagg tgtagttgct caaattccta attttcatcg
     5161 ctggttaaag aaaaacaaca ttgatgttga gctattaact gcaggagaat acaaacgcac
     5221 tctaacactt tttgcggaaa atactgaaaa aggtagaaaa aagtttcagg aagatttgga
     5281 aaaaattcat accgcattca gagagtatgt tcttaaaaat agaagtcaac tcgatataga
     5341 taaggttgca actggtgaac attggatagc aaaagatgct tttgatctcc ggctcgttga
     5401 taagttagct actagcgatg aatatttaat agaaaaaatg gcggagttta atgcattcaa
     5461 attaactgtc catgccaaat tgccaattat aagcaaggtg cttaaaccgg caatgagact
     5521 cattcatccg tggatttaaa aattagtact gaaacagctt gggaacgcta ttgaaattca
     5581 ataccgttaa atcaggcagc attccatagg aaacgagatc aattttatca tgggcttaaa
     5641 gattgatgat ggtagcggaa tttaaggaat taatgagcaa aaactttcaa aatctgccat
     5701 taaccaaaat ttgaatctcg gtatataatt aaagaataac aattttttct gaggagtaag
     5761 acatgagtga tttcaaatcg aagttaccag atcttaatga attaacatca atgaccgcga
     5821 aattatttaa agggattaaa accagtgtca gtgaaataat tcaggactat aagcaaaaaa
     5881 gagcacaacc tgttagtacc gaagagaaaa ccacagaaaa taagccagcg gaaagcaagg
     5941 ccgaaccaaa aacagaaagc aagcctgctg aaaacaaaac tgaatcaaaa gcagaaagca
     6001 aacctgaatc aaatacagaa gaaacacagg aaaaagagaa ataatcctgt gctagcgaat
     6061 aagtaatatg aggtgagttg tgtatctatc gctccattct ttctggtcga gttaagccat
     6121 atttacgcat tttttcaact aaagttgtcc ttctcatgtt gagataactg gctgcatgag
     6181 caacaaccca atctgactca tctaaggcct ggctaatcaa ggcaagttct gtcttgacta
     6241 aatgttcttt taaatcaatg ccttctgcat tagctattga ctcctgcttt attatttgca
     6301 acaaagtttc acgttccgcc cccgatgcct ctgagcaaga tggtttatat tcaccttgaa
     6361 accgatgagg taaatcactc ttgccaataa taccattagg gtaaagaata gataatctct
     6421 ccaccaaatt agctaactcc ctcacgttac ccggccaact gtattcgcat aaagcatcca
     6481 tagcatcggg cattaatcga acggtaggtc tgccctctcc ttcaattcga gaaaccagct
     6541 cattaaaaag cagtgataaa tcactacttc tattacgtag tggaggcatt tcaatgggaa
     6601 atacattcaa tctgtaaaac aaatcttctc taaatttccc ttcttgaata gcaatttcta
     6661 aatttctatg tgtggcagca atgatacgaa cattgacttc gatgctttta tttgaaccta
     6721 ctctttcaaa acaacgttct tgtaaaactc ttaataactt aacctgcata gctaatggca
     6781 tatctcctat ttcatccagg aatagagttc ctccattggc caactcaaaa cgtccttgcc
     6841 ttgatgtaat cgctcctgta aatgcacctt tttcatgacc aaataattca ctctccaata
     6901 attcccccgg gattgctcca caattgatag ggacaaatgg tttatttgct cgggatgaaa
     6961 gggaatgtat attgcgagca acgacttcct tacctgtacc tgactcaccc aaaatcaaca
     7021 cactggcctc ggtatcagct acttgctcaa tcaatttgcg tacttgacga ataccttcac
     7081 tattcccaac caagcttcta aataaagggg tcttatgagt gcccgcagtg ataaatttaa
     7141 ctgcttcttt agctatttga cacctatgga gtgatttaag catttttgca tagctgaatg
     7201 gaaatgataa acaatcaacg atatttctat ccgcgcattc agatgtagaa agcacagcat
     7261 caataaccaa tattggagtt tttggaaacc gtttaactaa tgtatccaat tcatccatca
     7321 ttttttcaaa acaagtagaa gctccaagaa ctatcacact tggattagga ttaacgctca
     7381 attgccaatt gccatattga gtgatctcag aggattggcc tataaaatca aaaatagtcc
     7441 gaagcttgtc acaacgttcc ttatcatcat cgataatata aatcctgtca ttgctcctca
     7501 taaacctatc cttaagtttg agtcatttaa tttaaagtat cggacacata atgaataact
     7561 gtcaaataat tgtcacaatt tattttaacc gtcataaaat ctatcaaatt aggccgtatc
     7621 gttataataa aaaaatacta atatatccta atgctaatta aataatgata gtcattacta
     7681 gcctgagcgt ttcaattatc tataacaaat aatcatctta aacgcattac actgattacc
     7741 aaaacataac tcctgacttc ctatggtctt cgtagcactc acttgagcct gtttcacgaa
     7801 atccttggct tgcatatatc ccatacgatt acagaacgaa ttagcagctt gtttaccaca
     7861 ggctttattg cgctccatac accaatccac tcggtaatca ttataacggg gtacggcaaa
     7921 ttttttctct cgataatggt aaggtttggg aggagtatgt gataaaccaa tagcacaagc
     7981 tattgtcata aaaccattac aacgccatcc tttgcattga gccttgctac ccaagtaatt
     8041 ggttaaaccg acgttatagg caatgacatt ttgagtggag taatcataac ccaacatttg
     8101 gcaataacga ttcgctacct cctttccgca ctctttccca tctaaagtac agtaatcgag
     8161 tcgttgccct aaataagtag gatgccaaaa atttctaaat gccaaattct gcttacttga
     8221 agctgttaac tgaccgcaaa aagaaaaaaa taaaagtata aaaaccagga aatttctaaa
     8281 attgctcatt ataacaatcc attaccaagt attagcacat gttagcttag gatgactggg
     8341 aggcacaaga gagatttatt cttcttttac tcgtgctgta atgaactttt gtcttattgc
     8401 attttctaga tacgactatg atttaaaagc tgaccaaaga caaaataact atatcaagat
     8461 aatttttcaa aggctataaa caatgttata aatagcatat gaacagaact tatcccctgt
     8521 aatcaaggca acaaatgttt ttaataggaa agtttggcta aattgaatga cagaattaaa
     8581 aacactttgt aacaaagagc ataggggctt aagcaaattc taatgaaaca agcttttcaa
     8641 tcatcacttt aaaagcccat atcaaaggct agtcgtttat gaaaaaatat ttaactttta
     8701 tagtgtcagg cttagcctta attgcaataa tatggttcat tcgccactta tggatcattg
     8761 ggactgtcaa ggttgtgcgt cctgtttatg gaacagcagt tcaagctgtc tatgccacag
     8821 gaacagtcga accaaccatc atgatgccaa ttgcctcaca aaatacagcc cgccttgtca
     8881 agttgaatgc agatgagggt tcggaggtag taaagggaca gatattagcc agcctggaag
     8941 atgaacacct acaacataac ttaaaagaac taaaagagca tgaaaaatta gcaaaaataa
     9001 gatacgagcg gaatgctcaa ttagttaaaa aaggagtcat ctccaaagat gagtatgatc
     9061 gcaccttgtc cgaatggcaa gcagccagcg ccgcagtaca cagaatagaa gctcaaatca
     9121 gttatttaca actgatagcc cctgcagatg gccgtatcat taaacgtgac ggtgaagttg
     9181 gacaactaat cccggcaaat caacctgtat tttggctttc ttgctgtgcc cccctaagag
     9241 tttccgctga agtcgatgaa gaagatattg tattggtcag agttgggcaa aaagtattga
     9301 tcagatcgga tgcttttcca aatcaaatct tttatggccg tgtcaaaagc atcaccccta
     9361 aaggcgatcc aatagccaga agttacagag ttcgcattag tttttcaaaa aataatcctt
     9421 tattgattgg tatgacagct gaaaccaata ttattgtcca tgaaaagaaa aatgccttat
     9481 tgctgcctgt aagtagtatt tctgaaaaca aagtttggat tgtagaggat gatgggaaac
     9541 tctctcaaaa tacagtgact attggcgcaa gaggattaaa acaagccgaa attttaaaag
     9601 gtgtcaatat taacgacctg gttgtcttac aacctgataa taagctaaaa aatgggcaaa
     9661 aagtgcgttt cattcttgta aaacaggaag aataatgaag ctatttttaa agatcgcttt
     9721 aaaacatttg cttgcacgca aacgacaaag cctggtttct ttgatgggaa ttgtattggg
     9781 ggtagccttc tttttaacaa tttcgtcact gatgcagggg tctgaaaaag atttcatcaa
     9841 acgcttagtc gataattcac cgcatatcac gatcatcgat gaatatcgaa accctcgtat
     9901 acaacccatt caacagctgc acacacatgg tgctattgaa attcgcagtg ttaaaccatt
     9961 aacagagcct cgagggatta gagggtatga acaaacaata aataatttat cgcttaccat
    10021 cccgggaatt attatttctc ctgttctaat tggacaagcc ttaattagtt atgcagggaa
    10081 agattattcg gtctctctaa atggtatgat ccctgaagag ataaaaaaag tttctactat
    10141 cgaaaattat atgacttcag gttcgattga tgatctcata gtcaatcccg atggtattgt
    10201 cttgggaagc gagctcttgc gtaaattatc cctgacaaaa gatgataaca ttactgtgac
    10261 agcgcccaat ggtcaaatac gcactttcaa aattcttggc gtatttcgca ctggccgttc
    10321 agattttgat accaatcaag cgtttattag tattaaacgc gctcaagcat tattaaaccg
    10381 acctaaccgc tccaataaca tcattataaa actgcctaac ccctatcagg cctatgacat
    10441 tgccgctcaa attgaaaaac agataggcta taaaactatt tcctggcagg aaaaatcgga
    10501 agatttgctc aatacactag tcattcgaaa tatcattatg tatagcgtag ttagcgctgt
    10561 tttaattgtc gctgcatttg gaatttataa tgttatttcg actgtagtaa tggaaaagca
    10621 cagagacatt gccatactta aatcaatcgg tttccaaaaa catgatattc aatttatttt
    10681 tatcatagaa ggttttcttc taggactggt tggatgtttg ttaggactgc ctttgggaag
    10741 tggactgatg tacagtctca tgcaagtcca attcaagccg ccaggaagct ctgaattgat
    10801 taatatgcca ctcgattgga gttatttaca atttgtcatc gccacagctt ttgcgatgtc
    10861 cgcctcaatc atagccgccg ttcttcctgc tcataaggca gcgcttgttc atcctgttga
    10921 tatcctgcga ggaggaggag gaggaatgtg aacaagattg tcataaaaac aaaagagctt
    10981 tcgcgtcaac tgtcaggtga ggttcccgtc actttagtca aggacataaa tcttgaggtc
    11041 agagaaggtg aatttttagt aatttctggc ccctcaggtt ctggcaaatc gtcattgctt
    11101 tatttgctag gcttgcttga tagcccttct caaggaacaa tttggctgaa taatgaagaa
    11161 gtttcaactt atgaagaaga aaaaatggcc tggttgcgct tgactcaaat tggttatgtc
    11221 tttcaatttc attttctatt acctgagttt acggccctgg agaatgtgat gttgcctatg
    11281 caacgtctgg gcaaactaag tctgggggaa atgaaaaacc gagctaccaa gcttttaacc
    11341 agcctagggt tagaggatca attatcaaaa cttcctaaac agctgtcagg agggcaaagc
    11401 cagcgcgtcg ctattgcacg agctctggcc aatgatccca tattgctctt ggcagacgag
    11461 ccaactggaa atcttgatac cgtttcaagt accaatgtgc aaaatatact aaaagaactt
    11521 gctcatgaat acagaagatc ggttatcatc gtaacacacg atatgaattt tgctgctaca
    11581 gcggacagag tgattcacat tgtcgatggc aaaatgcaag aataacctca cttcttgcta
    11641 gtccatgctt acttcatcag tttcatcctg atctattctt caggactaac gaaaaacccc
    11701 aaggtcgagg caaaaaatgt ttttaatgag ggagttaaga taaactaaat gaccgaatta
    11761 aaaacatttt ttaacataga gattggggtt ttgcgtaggc cctgttcttt ttaaatcgga
    11821 ttaggactat ctataaacca atgttccaag tcaaaatagt ttgcaagata aacaccgagg
    11881 gattgaatgc cgaaacgttc cgtcgcatga tgaccacaag aaaaatattg tatacccaat
    11941 tccatagcct gatagaacgt tcgttcagaa acttcaccgc ttaaataagc atcaacgcca
    12001 agttggtaag cttcttctat aaagtcctgt gctgctccag tgcaccatgc tatggagtga
    12061 atcatttttt catttcccgc aatatgtaca ggataccggc caagtttgtg ctctaaaaaa
    12121 ctggaaaact gttcgctgtt cattgccttt gataattttc cagaccaaag gagattggca
    12181 gtattgttga ccttatgcat ctcaggggac tctattaaca gcaaattcgc caggctggca
    12241 ttattcccta gctcgggatg acaatccaaa ggcaaatgat aggcaaataa attcatgtta
    12301 tggccaagta gcttagcaat ccttcgtctt ttcattccgg aaataacagg attttcacct
    12361 ttccagaaat aaccatggtg aactaacagt gcatcagctt gctgttcaat agcttgggaa
    12421 atcacgtcct ctgatgcact gactgccgta catatacgct taatcttgtc tttgccttca
    12481 acttgaattc cattaggtgc ataatcctgg tattgagagc aattcaagaa atccagtaaa
    12541 aaaaggctta attcatctct tgtaatcacc tgtcagaaac tctcttaatg tccgctccca
    12601 atagagataa cttttcttct attcgctcat aacctctatc aacatgataa atccgctcga
    12661 ctactgtttc cccttcagca accaggcccg cgagaattaa actggctgat gcccttaaat
    12721 cagtagccat taccggtgca ccagttaatt tttcaacacc atgaacgata gctgtatttc
    12781 cattaagttg gatattagcc cccattctct gtaactcttg aacatgcata aatcggtttt
    12841 caaaaatagt ttctataata gttgaagaac cttctgctac ggaattcatt gccataaatt
    12901 gagcttgcat atccgttgca aacgcaggat aaggggcggt tgcaatatta actgcctgag
    12961 gccgcttgtt atgcatattt aaactgaccc aatcctcacc aatcgttaat tccgcgcctg
    13021 cctcttcgaa tttacataat tgagataata aagtatcagg acgtactttc ttaacagtca
    13081 cttgaccacg agtaagcgct ccagctgcca aataagtgcc cgcttctatt ctatcggaca
    13141 tgaccgaata agtcccccca tttagtgatt ccacaccttc tacttcaata gttgaagtac
    13201 ccgcgcctcg aattttcgct cccatttgaa tcagaaagtt agccaaatcc accacttctg
    13261 gttcgcgcgc agcattttta atcgtagtaa tgccttctgc caacacagca gccattaaca
    13321 cattttcagt tcctgtgaca gtaactgtgt caaacatcaa gcgtttacct tgcagacgtc
    13381 cctttttaca acgagcattg atgtagccat ttttaaccgt aatgtctgcc cccatggctc
    13441 ttaatgcttt taaatgcagg tcaactggtc ttgttccaat ggcgcatcca ccgggcaaag
    13501 aaacatctgc cttaccgaag cgcgccagca ttgggcctaa taccaaaatg gaggcacgca
    13561 ttgttttgac cagatcataa ggagcaacaa actcattgac ttgactggag tcaacttgaa
    13621 cattcatctt ttcatcaaca attaagtgag ctcccaattg ccctaacagt tccatcatgg
    13681 tagtgatgtc tttaagatga ggtacattag atattgtgac gtgatcactg gctaatagac
    13741 tggcagccat gattggaaga gcagcgtttt ttgctcccga gatgaccact tcaccatgta
    13801 atgctttacc accattaatc aataatttat ccattttgct tcccccattc ttcttttgtc
    13861 catgtcttca tactgatagc atgaagcctt ccggtggtaa taaagtcttt aagctgggca
    13921 taaacccatt gctgtcttgc tactttagac ttcttaagaa aaatatcgct cactacagtt
    13981 acctgatatt gataaccatc gccctctacc ttgacataaa atacatcgtc aatttcatta
    14041 agtctttgtt caatttcttc attacttaac ataaacttag ctcactaatt aaaataaata
    14101 caaatttttt atacttttat tgataaaaaa taatgaacat aactgaatca ttatcaaaaa
    14161 aatccgtgtt tagaccatca ttctctaaag atttagacta tttcaccaac cacaacatta
    14221 cctcaaaatt catgggaatt tatcaattca ggaggacaac tagagtctgt taatttttct
    14281 ctggcttgaa acccaaaact ttgattttta agcaccgtag cagaaatata aaaccattat
    14341 taagcaacgg aacacagaaa accaacgctt taaagccaag acagtagaaa tgtcgataga
    14401 ttctagcgct ctaaagggat aagctctatg gaataataaa tatttctgac tcagaattgt
    14461 ttcaagatct ttgtaaaata gtcttcactc cacagaattc agccagagat tgtgtttcag
    14521 gagatattcc tattacatca aaaaccttat tattttgttt acataatttt ctagcttcaa
    14581 taattaaagc aagtccagca ctatcgcaat gagtcacctc acttaagtca aggcaaaatc
    14641 tctctctggt atcttccatt aaagccttat acagcttgtc acggaccgat accactgatt
    14701 tgaatgtaag ctcagcgcca ggtttaaagt tattaatttc cacgatcagg aagccttctt
    14761 aatctgtttt tgttgcattt ccttaatcac ttgactaatg ctggaatgtt gcagtgcctg
    14821 tgcaaattgc gatctaaaac tttgcaacaa actaacacct tcaacgctta tatcataaat
    14881 tttccatttc ccattcttgg acacaagact ataacttaat ggtatatttt gccctgcaga
    14941 acgcacaatc acgctattta cacgaataaa ccgactattt aaagagccgc gtaaaggtaa
    15001 aaactgcaca gtttcatcag aatattgggc caatgggcta gaatatgtac gaatcaccaa
    15061 gcgagtaaat gcttttgaga attcaaccct ttccgcttca ctagctttac tccaggcttg
    15121 tcttcccaag actgaacgag acataccagc aacgtcaaca ttgggtaaaa gattctcctc
    15181 gactgctttg taaataatct caggattact ttttaaacgc gctttatttt ctttgagaat
    15241 agaaataatt tgattagctg cacgttctaa catagggata ggagaggtct gagcaaccat
    15301 tgcttgagac aacgtcatca acacaactaa taaaattgtt ttaaaaactc tcatggttca
    15361 cctattttt
//
LOCUS       DACTJE010000067        15330 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.067, whole genome shotgun sequence.
ACCESSION   DACTJE010000067 DACTJE010000000
VERSION     DACTJE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15330
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.067"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..167)
                     /locus_tag="I9760_001613"
     CDS             complement(<1..167)
                     /locus_tag="I9760_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAT5043384.1"
                     /translation="MQNEYSQFEQRKRDHIELALMPANQSSELNPFDHFSLVHEALPD
                     LDFKDISIQSIR"
     gene            335..1393
                     /locus_tag="I9760_001614"
     CDS             335..1393
                     /locus_tag="I9760_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2050 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043385.1"
                     /translation="MKSKLEYHFSANADGDESCIEDVDGNEFTPNGSPAHGWTAWTRF
                     FGHQNQPPKYVVKQPRPDKVRSSSDLRDKKLNNEWNRARYQHELDIWNLVYPDNKAYL
                     FTDKGIRLVLPYLPGKTLFSFTSREEDQLTWCQLNHAVFCEFKRFTELGLKHCDLKYD
                     NFLIERKQDGTFRAYIIDLDSVGKLKPNDQDYDWNIVLASLFDDTGLKSPDVCPPGYI
                     ALKSIDVPAENENEARSKLDKIKKELSQKKPTLVRVKNCQQNKLDYYFVYGCLYNGQW
                     GFTKLDEKIISSVNLSNFSGALVDCRAASVIGNHIKLKQGHISYRLDLYINCLNEKIA
                     ELTLLKSQNSPKKLYIPF"
     gene            complement(1531..1830)
                     /locus_tag="I9760_001615"
     CDS             complement(1531..1830)
                     /locus_tag="I9760_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043386.1"
                     /translation="MGRFIFKGTSLIKLMLAFTLLIGALILVTPANAKAVNRDINPII
                     NKSNPTQMAYWVRYRYWGPYYYRGYYYRPYYYRWGYPYYRPYYRPYYRPYYYYRW"
     gene            complement(1957..2781)
                     /locus_tag="I9760_001616"
     CDS             complement(1957..2781)
                     /locus_tag="I9760_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043387.1"
                     /translation="MNEKLIETQSNLIKQAETELIQSNQLLESLQQEKGLQEESDLDE
                     LDSLKSLILVLQYRLDTQNALSQLGHLQQYLLQLLGVEAKHSFEFNIERLAFALGTDE
                     LKQILAMLNHLVDSLLRVIAQKQLKESQDKKKSLQQTKQFKPIDTLVNVLNKQKLFMA
                     KLDELKLALENIGGAPQPGIVYDHIAALQGPISRFYQALQHGLGLSNSLYQQLQKKNQ
                     LSYQLPDTLLKPEHVIRNMNFHPESQRLFKTTLERNKIENLEERAAAKRLGHFFNH"
     gene            2902..4026
                     /locus_tag="I9760_001617"
     CDS             2902..4026
                     /locus_tag="I9760_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HAT5043388.1"
                     /translation="MEQTSAQIVFEQKNATYQCCGSWSVLTLDGLPKKFSTSPLPDSQ
                     QITINGEKISAFDSAGALALKKCIDELKKRNNQIELVNFSDSQQQMLELIESKQDILS
                     YQLPAVSKEGVLYQIGKESENKLHQVDGLIILIGDLSTRLFEAFAYWRRFHLPSIVSV
                     IDSAGLKALPIIGLLSFLIGVVLAYQMGLQLETYGANIFIAYLSGMAIFREFGPLITA
                     IIVAGRTSSSFTAQIGSMKINEEVDAILTMGLSPTELLVLPKVLGLLIVFPLLIFWAD
                     VFSIWGAMIMSKNMLNIGFEDFLSRLRDSVGLNQMMLGLYKAPAFSILIALVGCFQGF
                     RVESSADSIGSQTTKSVVQALFLIIIADAAFSIAYSWMGL"
     gene            4027..4764
                     /locus_tag="I9760_001618"
     CDS             4027..4764
                     /locus_tag="I9760_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT5043389.1"
                     /translation="MSEPVIEIKGMKNCLGGQWVHTDVNLTVERGEILAIIGGSGSGK
                     TTILRSLLMLLKPSAGSIKVFGEEIYNLDTEQAFRLRRRWGMLFQHSALFSAMTVLEN
                     VMFPMREFTTLDQDFMKELALLKITLVGLSKEAAVKYPSELSGGMQRRAAAARAIAMD
                     PELLFLDEPTTGLDPRGARLFDDLVVFLRDALNLTIVMVSHDIESLKRTTDRVAFVGD
                     GKILSVEPINELMKNKNPLIAEYFSKF"
     gene            4794..5717
                     /locus_tag="I9760_001619"
     CDS             4794..5717
                     /locus_tag="I9760_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HAT5043390.1"
                     /translation="MESKTNYTIVGLIVLILTAGLLSAGLWLSVGFNQKEYTSYTVYL
                     KESVSGLSVESPVKFNGVQVGYVKEIKLNKNDPRQVELLLNIEKSTPITTSTSATLIT
                     QGITGVTYVGLSAGSSELTPLRKMPGEPYPVIPSKPSLLNQLDAVLKEVAENVGAVSE
                     KAQLIFNEENADNVRKSLANIERITEIIADKGQTIDSSLNNLDVFVANMANASKQFPQ
                     LIKDLKTGISKFKSLADNMSAAGKDVSKTMIAGKNTIDQISQQAIPPAVILLRRLNAI
                     SANLEKVSNEMRQNPSVIIRGTKAPKLGPGE"
     gene            5721..6323
                     /locus_tag="I9760_001620"
     CDS             5721..6323
                     /locus_tag="I9760_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043391.1"
                     /translation="MRVLSSVCFILGILTLMSCSPVKVPVTNEYQLTAYSTKQLTRKP
                     RPITIQVTVPEAVAGYQTEEMLYMKKPFKLEPFVKNAWTSPPADMLFPLLVQSLQSSG
                     YFYAVTSSPYSEEADYRLDTQLLKLEQNFIKKPSVLEFSAKIVLTHISDNQIIGSRIV
                     SLQIPCSQDTPYGGVIAANQATFRFTATATDFVVSHIKRD"
     gene            6421..6951
                     /gene="pal"
                     /locus_tag="I9760_001621"
     CDS             6421..6951
                     /gene="pal"
                     /locus_tag="I9760_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAT5043392.1"
                     /translation="MKAGSFYKLGLLVASAVLVAACSKTPGSADGGAAVGDGDATAQG
                     LGQMTHFAGQEPGESYTTQAPHNQLYLFAYDDSTLASKYLPSVNAQAEYLKTHPGARV
                     MIAGHTDERGSREYNVALGERRADTVAEILRMAGVSRQQIRVVSYGKERPANYGHDEA
                     SHAQNRRVEFIYEATR"
     gene            6948..7916
                     /gene="ybgF"
                     /locus_tag="I9760_001622"
     CDS             6948..7916
                     /gene="ybgF"
                     /locus_tag="I9760_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HAT5043393.1"
                     /translation="MINCKKSIITLCFVLMLPFASWAEAPVIDDSENFAMIDRQEEYE
                     APLVNPKYDNPQIENAELDGSQNDNYSTDTSQSYDEPALVKEDRSTISDNAKLIDKIQ
                     QLQKEIQELRGQLEVQAHDLKLLQQQQVAFYKDLDSRLSNSSTSAKTVQNDKPATDIS
                     LGSNSPETLKVASPQIKAGSSNGKPQPVVAVSRANPADEQISYLAAYELVKNKRYDEA
                     IKSMQTFVQKYPRGGYTANAEYWLGELYLVKKDYSKAIEHFEIVLQQYPSSSKAAASL
                     LKSGYAYAEKGDKQEAKKRFQQVVKTYPDTPTAQLASSKLEAINAL"
     gene            7913..8566
                     /gene="queE"
                     /locus_tag="I9760_001623"
     CDS             7913..8566
                     /gene="queE"
                     /locus_tag="I9760_001623"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAT5043394.1"
                     /translation="MKRFNEQLRITEIFHSLQGESVTVGLPTVFVRLTGCPLRCQYCD
                     TAYAFSGGEVVEIDDILNKVASYQCQHVCVTGGEPLAQPGCILLLSKLCDAGYSVSLE
                     TSGARDIASVDQRVMIVMDLKTPDSREADKNLLSNLSFLKPTDQIKFVLCSRTDYEWA
                     CSMLSEYQLAERVQLLFSPSWNQLNPTNLANWIIQDKLPVRFQLQLHKILWNDAPGH"
     gene            8584..9531
                     /locus_tag="I9760_001624"
     CDS             8584..9531
                     /locus_tag="I9760_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAT5043395.1"
                     /translation="MHWFAQAPANIALIKYMGKKDENSNLPDNSSLSYTLSNLLSSVK
                     LEKLPTKKDIWEPLTIPGAPEFNLSVEAQKRFIDHLVRLKEYFGYVGGFLIQSSNNFP
                     HSSGLASSASSFAALTKCASIALSELTQKPLPSIDEQAQLSRLGSGSSCRSFYAPWAL
                     WTGDKVSAIDLPYKDLLHQVIVISSQEKEIPSRVAHKLVKTSPFYETRSERAEANLKL
                     LLNAFENKDWTSIYQICWHEFLDMHQLFKTCEKPFSYITDNTLHILSVIEKFWNEKGD
                     GPVVTMDAGPNVHLLYRSDQTDLARQFKSDHLVGNYDVL"
     gene            9521..10399
                     /locus_tag="I9760_001625"
     CDS             9521..10399
                     /locus_tag="I9760_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAT5043396.1"
                     /translation="MFYDFETTTFGKWILAGEHAVVRGHEALVFPIKERQLTLKYNPS
                     SPSLSAEFQGINGSDMHLLFWSVLERGMHLLGRSLNELGGHFYLETNIPVGVGMGASA
                     ALCVAMSRWYCAQQMIQDNQCNEFAKQLEHLFHGKSSGLDVAGVASDTGVYFKSGLCT
                     PVKQTIYPYWYLSSCNQIGITSHCIQQVEYLWNKNPELAHQVDKQMVEAVQEAKAVLE
                     EGGSNALNRLAKAINKAADCFLQWGLVSEGLQQHMNHLFADGAIAVKPTGSGGGGFVL
                     SLWDKIPSSHTELIPV"
     gene            complement(10412..10681)
                     /gene="ftsB"
                     /locus_tag="I9760_001626"
     CDS             complement(10412..10681)
                     /gene="ftsB"
                     /locus_tag="I9760_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAT5043397.1"
                     /translation="MRPIFIILIVALVALQHKLWLGDGNIIQWIKLEKKLEAHKSQND
                     KLAARNKALEADIKELKSGDQALEEQARYELGMIKQNEVYYQFVD"
     gene            complement(10906..12174)
                     /gene="eno"
                     /locus_tag="I9760_001627"
     CDS             complement(10906..12174)
                     /gene="eno"
                     /locus_tag="I9760_001627"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAT5043398.1"
                     /translation="MHIHKIQAREILDSRGNPTIEADVTLTTGIIGRASVPSGASTGS
                     REACELRDNDPKRYAGKGVQKAVKHVNNEINQALQGLSVEDQENLDRILCQLDNTENK
                     SHLGANAILATSLACARARALSLNQPLYMTLNQGDMMTMPVPMMNILNGGAHADNNVD
                     IQEFMIMPIGAPDFPVALQMGTEIFHVLKSVLKKQGLNTAVGDEGGFAPNIQSNRQAL
                     DLLSEAIEKAGFRLGEDIVFALDVAASELFNEGFYHMYSENQKFDSHQLIEYYANLIS
                     SYPIVSIEDGLDEKDWSGWKQLTTHLGNKVQLVGDDLFVTNPKILREGIAQGIANAIL
                     IKVNQIGTLSETRQAIKLAYDNGYRCVMSHRSGETEDTFIADLAVASGCGQIKTGSLC
                     RTDRTAKYNQLLRINELASLPYAGKNILKR"
     gene            complement(12206..12808)
                     /gene="maf"
                     /locus_tag="I9760_001628"
     CDS             complement(12206..12808)
                     /gene="maf"
                     /locus_tag="I9760_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HAT5043399.1"
                     /translation="MSKFLQQKPIILASSSTIRHKLMKSLGLDFLVVPSNCNEEEIKT
                     RHNSVELVELGITLAKIKALDVSQHYPEHYIIAADQLCVADKRVFNKPLNHQTAVSHL
                     RELSGKQHQQIACLCIVKESKILWQYHETATLTLHHLSEKTIEAYLQAEKPYQSCGAY
                     QYEGLGKWLFKEVQGSEDTILGLPLMPLVNALVNLKVVGI"
     gene            complement(12801..13559)
                     /locus_tag="I9760_001629"
     CDS             complement(12801..13559)
                     /locus_tag="I9760_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAT5043400.1"
                     /translation="MLSATSLKIIFAVSIFIVILIAGWYPFKKRLKDDKHIDFPIGET
                     LATGVFLGAALLHMLPESNTLFKEMGYNYPFAFIITGVVFLIFLWFEHLGKELYHHHD
                     AEHPAFAILAWAMLSVHSLMLGAALGFTQYNSMIIMLFLAIITHKWAESLAIAIQLNK
                     SSMSTRKSMVFFFFFSLMTPLGIYFGWYFGHGVETNSLFDPILIAASAGTFLYLGTLH
                     GLERCVMVERCCNLSDFSFVIIGFLLMASVAIYV"
     gene            complement(13675..14817)
                     /locus_tag="I9760_001630"
     CDS             complement(13675..14817)
                     /locus_tag="I9760_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAT5043401.1"
                     /translation="MYPHDRYWQAKKVTLIGAFINALLGVVKMIGGFLYHSHALIADG
                     VHSISDLITDVTVVFASKYGSVSADDSHPYGHQRIETAATLLLSLLLILAGCGIAWDA
                     LDELLKSDHTMPNWLSIPIVCFSIIANEALFHYTRHVGKKISSQLITANAWHHRSDAA
                     SSIVVLIGLIGSLAGLVYLDAIAAVIVSLMIIKMGWSYGWNSVKELVDTAVDAELFSQ
                     IERVIQSIDGVKRIHQLRSRFMGSDVLIDVHILVSPKISVSEGHYIAQHVHHTLVKEI
                     DCVKDVIVHVDPEDDEISCPSLHLPNRAILQEKFFNDIRHDFPQILFWNIHYLNGKMS
                     LDIFCSNDFNQWKKLHNRVISTLKSQIDIKEVRLFGLHEEMHHPSN"
BASE COUNT         4701 a         2759 c         3095 g         4775 t
ORIGIN      
        1 cggatgcttt gtatggaaat gtctttaaaa tcaaggtcag gtaaggcttc atgcaccaaa
       61 gaaaaatgat caaagggatt taattcgctg ctttgattgg ctggcattaa ggccagttct
      121 atgtgatcgc gcttacgctg ttcaaattgg ctgtattcgt tttgcatgcc tgaatttact
      181 tttgaaaaat agcgtcaatt ttagcataaa atcatgaaat agaccattgc atttctattc
      241 aacacagtga tttatttggt atcattaatt taaccatgag ttaactattt ttttcgttta
      301 gctagcttct tggtaacaaa ttaaggtaat tatcatgaaa tccaaacttg aataccattt
      361 ctctgcaaac gcggatggcg atgaatcctg tattgaagat gttgatggaa atgaatttac
      421 tccaaatggt tcaccagcac atggatggac tgcgtggact agattttttg gccatcaaaa
      481 ccagcctcct aaatatgttg ttaaacaacc tagaccagat aaagtccgtt cctcttcaga
      541 tcttagagat aaaaagctta ataatgaatg gaacagagca cgctatcagc atgaactgga
      601 tatatggaat ttggtttatc cagataataa agcctatttg tttacagata aaggaattcg
      661 attggttctc ccttatttac ccggtaaaac gctattttct tttactagta gggaagaaga
      721 tcagttaacc tggtgtcaac taaaccatgc agtattttgt gagtttaagc gctttactga
      781 gcttggattg aagcactgcg atttaaaata tgacaatttt ttgatagaaa gaaagcaaga
      841 tggtaccttt agagcctaca taattgatct cgattcagtg ggtaaactaa agcctaatga
      901 tcaggattat gattggaata ttgtcctggc atctctattc gacgataccg gactaaaaag
      961 tccggatgtt tgcccacccg gctatattgc tttgaaatca atcgatgttc cagcagaaaa
     1021 tgagaatgaa gcacgatcaa aacttgataa aataaaaaaa gaactatcgc aaaaaaaacc
     1081 taccttggta agagtcaaga attgccagca aaataagttg gattattatt ttgtttatgg
     1141 atgtttgtat aatgggcaat ggggctttac taaacttgat gagaagatca ttagttcagt
     1201 taatctatct aatttttctg gtgctttagt tgattgtaga gctgcttctg ttattggtaa
     1261 tcatattaaa ttaaaacaag gccacattag ttacagactt gatctttata tcaattgtct
     1321 gaatgagaaa atagctgaac tcactctact caaatctcaa aactcaccta aaaagctgta
     1381 tatcccattt taaaccagaa cctgtttcta ccatgaaggg atcaaagcgt gctgctgtgt
     1441 tataagtata atatcctctt cttggcaaag ccttcttagg ctatatcacc atagctatat
     1501 tctatacata ggccagcagc gtcttatcgc ctaccagcga taatagtaat aaggtctgta
     1561 gtagggcctg tagtaaggtc tgtaatatgg ataaccccag cgatagtaat acggtctata
     1621 gtagtatccg cgatagtaat atgggcccca atatctatag cgtacccagt aagccatttg
     1681 agtcggattg gatttattaa ttatcggatt gatatctctg ttaactgctt tggcatttgc
     1741 aggcgttact aaaatcaaag ctccaatcag aagggtaaaa gctagcatca gttttattaa
     1801 agaggtccct ttaaagataa aacgtcccat tttttactcc ttaaagcgta ttttatttaa
     1861 gtatagacta cacgggatta tttgattaaa ttttgtacac aatcattact gggcatgagt
     1921 ttaaatttta ttgaggcagt aagtggttta ttagggttaa tgattaaaaa aatgcccgag
     1981 ccttttggcc gcagctcgtt cttcaaggtt ctcgatctta ttgcgttcca aggttgtttt
     2041 gaatagtcgc tgcgattcgg gatgaaagtt catattacga ataacgtgtt ctggtttaag
     2101 caaggtatca ggtaattgat aactcaattg attttttttc tgtaactgtt gataaagaga
     2161 gttacttaag cctaaaccat gttgtagtgc ctggtagaaa cgggatatgg gcccttgtaa
     2221 cgcagcaata tggtcataaa ctatgcctgg ttgtggagct ccgccaatgt tttcaagagc
     2281 cagctttaat tcatcaagtt ttgccataaa taatttttgt ttattcaaaa cattaaccag
     2341 ggtatctatc ggtttgaatt gcttggtttg ttgaagtgat ttctttttgt cctgagactc
     2401 tttaagttgc ttttgggcaa taacacgtaa caacgaatct accaaatgat taagcatagc
     2461 cagaatttgt tttaattcat ctgttcccag agcaaaggcc aatcgctcaa tattgaattc
     2521 aaaagaatgt ttggcttcta cccccaataa ctgaagcagg tattgttgta aatgacctaa
     2581 ctggcttaag gcattttgtg tatccagtct gtattgcaaa accagaataa gggatttgag
     2641 tgagtccaat tcatccaagt cggattcttc ctgtagtcct ttttcttgtt gtaaagactc
     2701 cagcagctgg ttagattgaa tgagttctgt ttccgcctgt ttgatgaggt tagattgggt
     2761 ttcaattaac ttttcattca tgaagataac ctggatataa aagagcacta tctatattat
     2821 tatgatacca tgaatcgtaa gtttttgcgg tcttgtggta ttttgtatta ttctaggaaa
     2881 attcaataaa aagaaataaa tatggaacag actagcgcac agattgtttt cgaacaaaag
     2941 aatgcaacat accagtgttg tggttcctgg tctgttttaa cccttgatgg attacctaaa
     3001 aaatttagta caagcccatt acctgattca caacaaataa ccataaacgg tgaaaaaatc
     3061 agtgcttttg atagtgctgg agctctggca ttaaagaaat gcatagatga gcttaaaaaa
     3121 agaaataacc agattgaatt agtcaatttt tctgatagcc aacagcaaat gctggagctc
     3181 atagagtcca aacaagatat tctttcctat caattacctg ctgtttccaa agaaggtgtt
     3241 ctttatcaga ttggtaaaga gtcagaaaat aaattgcatc aggttgatgg tttgattatt
     3301 ttgataggag atttaagcac aaggcttttc gaagcgtttg catattggcg gcgatttcat
     3361 ttgcccagca ttgtttctgt tatagattct gccgggctaa aagcgttacc catcattggc
     3421 ttattgtctt ttttgatagg ggtcgtttta gcttatcaaa tgggtttgca attagaaact
     3481 tatggtgcca atatttttat tgcctattta tctggcatgg caatttttcg ggaatttgga
     3541 cctttaatta cagcaatcat tgtagcgggg cgcaccagct catcattcac agctcaaatt
     3601 ggcagcatga agattaatga agaggtagat gccatcttga ccatggggct ttctcccact
     3661 gaattattag ttttacccaa agtcttgggt ttattaatag tgtttccttt attaattttt
     3721 tgggcagatg tctttagcat ttggggggca atgatcatgt ccaaaaatat gttaaacatc
     3781 ggttttgaag attttttaag ccgcttaaga gattccgtag gacttaacca aatgatgctg
     3841 ggtttgtata aagctcctgc cttctcaata ttaattgctt tagtggggtg ttttcagggg
     3901 tttagagtag aatcaagtgc tgatagtata ggaagccaaa caaccaaaag tgttgtgcaa
     3961 gctttattct taattattat tgctgacgct gctttctcca tagcgtatag ctggatgggt
     4021 ttataaatga gtgaaccagt cattgaaatc aagggaatga aaaattgtct tggcggtcaa
     4081 tgggtgcaca ccgatgttaa tctcaccgta gaaaggggag aaatcctggc tattataggg
     4141 ggctctggaa gtggcaaaac caccatatta cgcagtttgc tgatgttatt gaaaccttct
     4201 gcgggtagta ttaaagtatt cggtgaagaa atttataatc ttgatacgga gcaagctttc
     4261 aggcttaggc gtcgatgggg catgttattt cagcacagtg ctttattttc agctatgacc
     4321 gttctggaga atgtgatgtt cccaatgagg gaattcacta ctttagatca ggattttatg
     4381 aaagaactgg cacttttaaa aataactttg gtaggcttat ctaaagaagc agctgtgaaa
     4441 tatccttccg agttaagtgg gggaatgcaa agaagagctg ccgcggcaag ggcaattgca
     4501 atggatccag agctattatt tctggacgaa cctacaacag gattggatcc caggggagcc
     4561 aggctttttg atgatttggt tgttttttta agagatgcgt taaatttaac catagtcatg
     4621 gttagtcatg atatagagtc tttaaaaagg acaacagatc gtgttgcatt tgtaggtgat
     4681 gggaaaattc ttagtgttga acccattaat gaattgatga aaaataaaaa cccgctaatc
     4741 gctgaatact ttagcaaatt ctaagaaaca ctataacaga ggggacaact tacttggaat
     4801 ctaaaaccaa ctacaccata gtaggcctca tcgtacttat tctgacagct ggcctattgt
     4861 ctgcgggttt atggctctcg gtcgggttta accagaaaga gtatacttct tatactgttt
     4921 atctcaagga gtctgtttct ggtctcagtg tcgaatcgcc tgttaaattc aatggtgtgc
     4981 aggttggtta tgtcaaggaa attaaattaa ataaaaatga tcccaggcaa gtggaattat
     5041 tgttaaatat cgaaaagagt acgccaataa caaccagtac ttccgcaacc ttaattacac
     5101 agggtattac aggagttact tatgttgggt tatctgctgg atcttccgag ctgactcctt
     5161 tacgaaaaat gccgggggag ccctatcctg taattccttc caaaccatct cttctgaatc
     5221 agttggatgc tgttttaaag gaggttgctg agaatgtagg tgcggtgagt gaaaaggctc
     5281 aacttatctt taatgaagaa aacgctgata atgtaagaaa atcattggct aatatagagc
     5341 gtattacaga aataatcgct gataaaggtc aaacaattga tagttcttta aataatcttg
     5401 acgtgtttgt agccaatatg gcgaatgcaa gcaaacaatt tcctcagttg ataaaagatt
     5461 taaaaacagg tatttcaaaa ttcaaatctt tagctgacaa tatgagtgct gcgggtaagg
     5521 atgtttcaaa aacgatgata gcgggcaaga atactatcga tcaaatttcc caacaggcta
     5581 tacctccagc agtcatttta ttgcgacgct tgaatgccat ttcagcgaac cttgaaaaag
     5641 tcagcaatga aatgaggcaa aacccttctg tgattattcg aggtacgaaa gcacctaaac
     5701 ttgggccagg ggaataaaat atgagagttc tcagtagtgt ttgttttata cttggaatat
     5761 taacactgat gagttgttca cctgttaaag tcccagtgac caacgaatat caattaacag
     5821 catacagtac caaacaattg accaggaagc ccaggcccat aacaatacaa gttacagtcc
     5881 ctgaagcggt agcaggatat cagaccgaag aaatgcttta catgaagaaa ccgtttaaat
     5941 tggaaccatt tgtaaaaaac gcctggacct cacctcccgc ggatatgctc tttcctttat
     6001 tagtacaaag cttgcagagt tcagggtatt tttatgccgt tacatcaagt ccgtatagtg
     6061 aagaagcaga ttatcgattg gatactcaac tattgaaatt agagcaaaat tttatcaaga
     6121 aacctagtgt tcttgaattt tcagcaaaaa tagtactaac ccatattagt gataatcaaa
     6181 ttataggctc gcgaattgtt agtttacaaa taccttgttc acaagatact ccttatggcg
     6241 gagttattgc ggcgaatcag gctacgtttc gatttactgc aacggcaaca gactttgttg
     6301 tatcgcatat taaacgtgat taggaatagc caaaaaataa aaagaattgt acaatggctc
     6361 tccatgatta tcatgtgtag tttctaaaaa ataataagaa gagtttaagg agattgtgga
     6421 atgaaagccg gatcgtttta taaactggga ctgcttgtag caagcgctgt attggttgca
     6481 gcctgttcta aaaccccagg cagtgcggat ggtggggctg cggtaggtga tggtgatgct
     6541 actgcccaag gtttggggca aatgactcac tttgctggac aagagcctgg tgagtcctat
     6601 acaacgcagg caccgcataa tcaattgtat ttgtttgcgt atgatgacag cactttggca
     6661 tcaaaatatt taccttctgt taacgcgcag gcagaatatt taaagactca cccaggtgct
     6721 cgagtgatga tagctggaca tactgatgag cgtggtagcc gtgaatataa cgttgctctt
     6781 ggtgaacgcc gtgctgatac tgtagctgaa attctgcgta tggcaggtgt tagcagacaa
     6841 caaattcgag tagttagcta cggcaaagag cgcccggcta attatggtca tgatgaagcg
     6901 tcgcatgcgc agaatcgacg cgtagagttt atttatgagg caacaagatg attaattgca
     6961 aaaaatccat tatcacccta tgttttgtac tgatgcttcc tttcgcctca tgggcagaag
     7021 cgccagtaat agatgatagt gaaaattttg caatgattga cagacaagag gagtatgaag
     7081 ctcctcttgt caatcccaag tacgataatc ctcaaattga gaatgctgag ttggatgggt
     7141 ctcaaaatga taactactca accgatacct ctcaatccta tgatgaaccc gcacttgtca
     7201 aagaagaccg aagtactatc agtgacaatg caaagttaat tgataaaatt cagcagcttc
     7261 aaaaggaaat acaagagtta cgtggccagc ttgaagtaca ggcacacgat ttgaaattat
     7321 tacagcagca gcaagtcgct ttctacaagg atctggattc tcgattatcc aattcttcaa
     7381 cttcagcaaa aactgttcaa aatgataaac cagcaacaga tatttcattg ggatctaatt
     7441 cgccagagac tttaaaagtg gcttctcctc aaatcaaagc aggatcatct aatggtaaac
     7501 ctcagcctgt tgtcgctgtt tccagggcta atcctgctga tgaacagata agctacctgg
     7561 ctgcatacga gttagttaaa aataaacgct atgatgaagc gattaagtct atgcagactt
     7621 ttgtgcaaaa atatcccaga ggtggttaca ccgcgaatgc agagtactgg ctaggtgaat
     7681 tatatcttgt taaaaaagac tattctaaag caatagaaca tttcgagatt gttttacagc
     7741 aatatccttc atccagcaag gctgcagcca gtttgttaaa atctggttat gcttatgccg
     7801 agaaaggtga caagcaagaa gctaaaaagc gttttcaaca agttgttaaa acatatcctg
     7861 atacaccaac agcgcaactg gccagctcta aattggaagc aattaatgct ttatgaaacg
     7921 atttaatgaa caattaagaa ttacggagat ttttcattca ttgcaaggag aatcagtaac
     7981 tgtaggtttg cctactgtat ttgttcgcct gacaggctgc ccattgcgat gtcagtattg
     8041 tgatacggct tatgcttttt caggtggtga agttgttgaa atagatgata ttttgaataa
     8101 agtagcttca tatcaatgtc agcatgtttg tgttactggt ggagaaccac ttgctcaacc
     8161 tggctgtatt cttttattaa gcaaactttg tgatgcaggg tactctgtct ctttggaaac
     8221 cagtggcgcc agagatatag cctctgtaga tcaacgagtt atgatcgtta tggacttaaa
     8281 aactcctgat tccagggaag cagataaaaa cttgctgtcc aatttgagtt ttctgaagcc
     8341 cactgatcag attaagtttg tgctatgcag ccgaactgat tatgaatggg catgtagtat
     8401 gctgagtgaa tatcagttgg ctgaacgggt acaactctta ttttctccga gttggaatca
     8461 gctaaatcct actaatcttg caaattggat aattcaggat aaattaccag ttcgttttca
     8521 actacaatta cataaaattt tatggaatga tgcacctggt cattgacctc aaggagacta
     8581 actatgcatt ggtttgctca ggcaccagct aatattgctt tgatcaaata tatgggtaaa
     8641 aaggatgaaa attccaattt acctgataat tcttctctat cttacacctt gagtaattta
     8701 ttaagttccg tgaaattaga gaagttgccg actaaaaaag acatttggga gccacttact
     8761 attcctggag caccagaatt taacctgtca gttgaagcgc aaaaacggtt tattgatcat
     8821 ttagtacgat tgaaagagta ttttggatat gtcggtgggt ttttaattca atccagtaat
     8881 aatttccctc atagcagtgg tttagcgagt tctgcctcca gctttgctgc cttgacaaag
     8941 tgtgcatcaa ttgcattaag tgaactcact caaaaacctt tgccttctat tgatgagcaa
     9001 gcacaattga gtcgattagg ttcaggctca tcatgccgtt ctttttatgc accatgggcg
     9061 ttatggacgg gcgataaagt ctctgcaatc gatttgcctt ataaagatct attacaccaa
     9121 gtcatagtaa ttagcagtca ggaaaaagaa ataccatcaa gggtagcgca taagctggtt
     9181 aaaacaagtc ctttttatga aacgcgaagc gaaagggcag aggcaaattt gaagttgtta
     9241 ctgaacgctt ttgaaaacaa agattggacg agtatatacc aaatttgctg gcatgaattt
     9301 cttgacatgc atcaattatt taaaacctgt gaaaaaccat tttcttacat tacagataat
     9361 actttgcata tattgagcgt tattgaaaaa ttttggaacg agaaaggtga tggccccgta
     9421 gttactatgg atgctggtcc caatgttcat ttgctttata gatcagatca aacagatctc
     9481 gcacgacaat ttaaatcgga tcatttggta ggaaactatg atgttttatg attttgaaac
     9541 caccactttt ggcaagtgga ttttagcagg tgaacacgcg gtagtaaggg gacatgaagc
     9601 tttagtattt cctatcaagg aaaggcaatt aactttgaaa tataaccctt cctcaccgtc
     9661 cttgagcgca gaatttcaag gcattaatgg ttctgatatg catttattat tttggagcgt
     9721 attagagcgc ggcatgcact tgttgggacg ttcactgaat gaattaggag gtcactttta
     9781 tttggaaacc aatattcctg ttggggttgg catgggtgct tcggcagcgc tttgtgttgc
     9841 catgagtcgg tggtactgtg cgcagcaaat gatacaagat aatcaatgca atgaatttgc
     9901 caaacaatta gaacatctgt ttcatggtaa aagtagtggc ttggatgtag ctggtgtagc
     9961 gtcagatact ggtgtatatt ttaaatccgg gctttgtacc ccagtaaagc aaaccattta
    10021 tccctattgg tatttatcct cctgcaatca aatcggcatt acttctcatt gtatacagca
    10081 agttgaatat ttatggaata aaaatccaga acttgctcac caagtagata agcaaatggt
    10141 agaggctgtt caggaagcaa aagctgtttt ggaagaaggg gggagtaatg ctttaaaccg
    10201 tttggcaaaa gcgataaata aagccgcaga ttgtttttta caatggggtt tggttagtga
    10261 gggcttgcaa caacatatga atcatttgtt tgctgatggt gcaattgcag ttaagccaac
    10321 cggttcaggt ggtggagggt ttgtattaag cctttgggat aaaattccct catcccatac
    10381 agagttgatt ccggtctagc tcctttcaag cctaatcgac aaattgataa tatacttcat
    10441 tttgcttaat catgccgagt tcatatcgag cttgttcttc aagagcctga tcaccacttt
    10501 ttaactcttt gatatctgct tccaacgctt tatttctggc agctaattta tcgttttgac
    10561 ttttatgagc ctccaatttt ttttcgagtt taatccattg gatgatattt ccatcaccta
    10621 gccatagctt atgttgtaaa gcaactaaag caacaattaa gataatgaat atggggcgca
    10681 tagagttttc tattgaagaa tgtatctaat tactattgta gtatcttatt accacgagga
    10741 gcaatatgtg ttcagggtat atagtattaa attttttatc gaactgatga ttttaaattg
    10801 tattttattt atattacctt tgatttaaat tagagctgat gccaagtttt attcagcact
    10861 caacatcacc ccaacttaaa cattaataat gctgaaagct tgaactcatc tctttagaat
    10921 gttttttcca gcatagggta aagacgccaa ttcattgatt cttagtagtt gattatattt
    10981 cgcagtcctg tctgttcgac acaaagatcc ggtttttatt tggccacaac cactggctac
    11041 tgcaagatca gcaatgaagg tatcttctgt ttctcctgaa cgatgggaca tgacacatcg
    11101 gtatccattg tcgtatgcga gctttatagc ttgcctggtt tcgcttaaag tacctatttg
    11161 attgacttta attaatatgg cattagcaat tccctgagca attccttctc gcagaatttt
    11221 gggattagtt acaaataaat catctccgac gagctgaact ttatttccca aatgggtggt
    11281 taattgcttc catcctgacc agtctttttc gtccagtcca tcttcaatac tgactatggg
    11341 ataactggaa ataagatttg catagtattc tatgagttga tgagaatcaa atttttgatt
    11401 ttcagaatac atatgataaa agccctcatt aaaaagctca gaagccgcaa catccagggc
    11461 aaatacaatg tcttccccta atcgaaagcc tgctttttca attgcttcgc taaggagatc
    11521 cagcgcttgg cgattggatt gaatattagg tgcaaaacca ccttcatcac caacagcggt
    11581 atttaatcct tgctttttta acacagattt cagtacatga aaaatttcag ttcccatttg
    11641 caatgcaact ggaaaatcag gagcgccgat tggcatgatc ataaattctt gaatatccac
    11701 attattatca gcatgagcgc ctccattcag aatattcatc ataggaacag gcatggtcat
    11761 catgtcaccc tgattaaggg tcatatacaa tggctgatta agagacaatg ctcttgctct
    11821 ggcacatgcc agagaagtag ccaggatagc attagctccc agatgggatt tgttttctgt
    11881 attatccaat tggcataaaa tgcgatccaa gttctcctga tcctcaaccg acaatccttg
    11941 taatgcctga tttatctcgt tattaacgtg tttaactgct ttttgtacac ccttgccagc
    12001 gtatcgtttt ggatcattgt ctctaagttc acaggcttcc ctgcttcctg tggaagcacc
    12061 tgaaggaacg ctggctcttc caataatgcc agttgtgaga gtaacgtctg cctcaatagt
    12121 tggattaccg cgagaatcta atatttcacg tgcttgaatt ttatgtatgt gcatgatctt
    12181 tcctaatctt tttatattta ttaacttaaa tacctaccac cttaaggttt acaagcgcat
    12241 taactaatgg cattagtggc aaaccaagaa tagtatcctc gcttccttgt acttccttaa
    12301 ataaccactt gcctaggcct tcatattgat aagcaccaca gctctgataa ggtttttccg
    12361 cttgtaaata ggcttcaatt gttttttcac ttagatgatg cagtgtcagt gtagccgttt
    12421 catggtattg ccaaagtatt ttgctctctt taacaatgca caggcaagca atttgttgat
    12481 gttgcttccc gcttaactct ctcaaatggc ttacagctgt ttgatgattc agtggtttgt
    12541 taaatactct tttatcagcg acgcataatt gatctgctgc aatgatatag tgctctggat
    12601 aatgttggct tacatccaac gctttaattt tagccagggt aatccctaac tcaactagtt
    12661 cgacggagtt atgccttgtt ttaatttctt cttcgttaca attcgatggt acaaccagga
    12721 aatccaagcc aagtgatttc atgagtttat gtcttatagt cgaagatgag gcgagaataa
    12781 tcggtttttg ttgtaaaaat ttagacatag atggccaccg atgccatgag taaaaagcct
    12841 atgattacaa agctgaaatc acttaaatta cagcaacgct ctaccatgac acagcgctct
    12901 agtccatgta aagttcccaa atagagaaat gtaccggcag atgccgcgat taatataggg
    12961 tcgaataaag agttagtttc tactccatga ccaaaatacc aaccaaaata aatgcctaag
    13021 ggtgtcataa ggctaaaaaa gaaaaaaaat accatacttt ttcgagtgct catcgagctt
    13081 ttatttaatt gaattgctat agccaaactt tccgcccact tatgagtgat gatcgctaaa
    13141 aatagcataa tgatcatgga attgtattgg gtaaaaccta aagcagctcc cagcataagc
    13201 gagtggacag aaagcatggc ccaggcgagt attgcgaaag caggatgttc tgcatcatga
    13261 tggtggtata gttcctttcc taaatgttca aaccacaaga aaataaggaa tactactccg
    13321 gtaataataa acgcaaatgg atagttataa cccatttctt taaataaggt gtttgattca
    13381 ggtaacatgt gaaggagtgc tgcacccaga aaaacacctg tagccaaagt ttcacctatg
    13441 ggaaaatcaa tgtgtttatc gtctttaagt cgctttttaa atgggtacca gcctgcaatg
    13501 agaatgacta taaatatcga aacagcaaaa atgattttta aactggttgc ggacaacatg
    13561 aagtcttcct actttaaaat gcgtatatat tatcctgaaa tgactaatcg aatctacagt
    13621 gcacagcagt attacattgt taaaatttag caattcttgc atgccttctg gatattagtt
    13681 tgatggatga tgcatttcct catgcaagcc aaagaggcgc acttccttta tatcgatttg
    13741 tgatttcagg gttgagatta ctcgattatg caattttttc cattgattaa aatcatttga
    13801 gcaaaaaata tccaaactca tttttccatt taaataatgt atattccaaa ataaaatctg
    13861 gggaaaatca tggcgaatgt cattaaaaaa tttttcctgg agtattgccc tgtttggtaa
    13921 atgcaaggat ggacatgaaa tttcatcgtc ttcagggtca acatggacaa tcacatcttt
    13981 gacgcaatca atttctttta ccagagtatg atgcacatgt tgagcgatat agtgcccctc
    14041 ggaaacggat attttgggtg aaaccagaat atggacatca ataagcacat cactacccat
    14101 aaaccggctt ctcaattggt gaatacgctt tactccatca atgctttgta taactcgttc
    14161 aatttgagaa aataattctg cgtcaacagc tgtgtctacc aactctttga cactgttcca
    14221 gccatagctc caccccattt ttataatcat caggcttacg atgacggcgg caatggcatc
    14281 caggtaaaca agccctgcca agctgccaat taatccaatc agcacgacta tggatgaagc
    14341 agcatctgat cgatgatgcc aggcattggc ggtgattaat tgggagctaa ttttttttcc
    14401 tacatggcgg gtgtagtgaa ataatgcttc gtttgcaatg attgagaagc acactatagg
    14461 aatagataac cagtttggca tggtatggtc tgatttaagc aattcgtcaa gtgcatccca
    14521 ggcaatccca cagcctgcta ggattagtaa aagggaaagc aataaagttg ctgccgtttc
    14581 aattctttga tggccatatg gatgagagtc atcagcgcta acgcttccat attttgaagc
    14641 aaatacaacc gtcacatcag taattaaatc agaaatggag tggactccat ccgcgatgag
    14701 agcatgagaa tgatataaaa aaccaccaat cattttgaca acaccgagta aggcattaat
    14761 aaaagcaccg ataagggtca cttttttggc ttgccaatac ctgtcatggg ggtacattgt
    14821 tgttcctttt tcaatcacaa aaataattta gatttttgta aataaaaatt agaagggaaa
    14881 ctataccatg ttatagcatg agttcctatt tatctcgtgc tgtcagttat agtcgactaa
    14941 atgtattaat aatttctttt tggtttttac tcgattacgc taggagaagt taaaaaaata
    15001 attgcagatg ttacccaaaa gcaaattgga aagttctgaa tcagttagaa aatagactga
    15061 ttttacaagg tgttttgcat tctgtgcaca ggattatcca cagattttgt ggataaatat
    15121 atctgaggga attaaattaa aaaagccagg taacccttac atggaaaggg ctatagaaat
    15181 ttcaatagta aaattagcaa gagttttgtg caattgattt ttgaagttat ccacagggat
    15241 aagacggaat gaatgttact tgtggaccgc ttatgttagc aaagaaaaaa ataaaaaaca
    15301 gtcttgactt caataatttt aattattttt
//
LOCUS       DACTJE010000068        15322 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.068, whole genome shotgun sequence.
ACCESSION   DACTJE010000068 DACTJE010000000
VERSION     DACTJE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15322
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.068"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            408..1736
                     /locus_tag="I9760_001631"
     CDS             408..1736
                     /locus_tag="I9760_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAT5043402.1"
                     /translation="MIKTFIDKKIQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKM
                     NHEAYRPEVQDQIRWLLDHPGFVTKACKQAEPYLYHVTREIQRRNLPGELALLPMIES
                     AYDPFAYSKVGAAGLWQIMPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVF
                     NGSWILSIAAYDAGEGAIGRAVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMS
                     NPRAYKINLPATPFRPYFEEVNIGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPY
                     KPFTLLIPIAKVTQFYFNLSHSPVDKRVSWRKHLVLPSDSLQSIARKYHTTVSLIKKL
                     NRLSEHSIRPNQILLIPSAKSTPALPFHEISKSKFLSQTPTKGKVYRVIHIVQANETY
                     NTLERLYKVTAQDIMKWNHIGSGEALVKGRQLIIWKKSSNLEWNRTFKKT"
     gene            complement(1862..2623)
                     /locus_tag="I9760_001632"
     CDS             complement(1862..2623)
                     /locus_tag="I9760_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAT5043403.1"
                     /translation="MKRIVFILLFISNVLVHSESLKVGVLQFAPPFSSISDKANHYYG
                     FVVALMNIICNRLQEKCEYIPIPKEGELKGLDKGVYHIVFSSTPITTSLPANYRYSLP
                     YLPSDGQFLTLKNNSIDTLNDINFKKVGFFKINFQQSPLLKKYETTNTLIEYTDPAEL
                     VNALTTKKIDAILINYQASRFIVNNLNPTEDDQLKLIGDKIPIGSGYGIIALKNQSAL
                     IDKINHILLEMEKDGSYLRIYNEYFGANPAYNLHH"
     gene            complement(2859..3449)
                     /locus_tag="I9760_001633"
     CDS             complement(2859..3449)
                     /locus_tag="I9760_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1124 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043404.1"
                     /translation="MRMKMFSKTIPLTSQEAFEILCTTDYLKKISKIIFNFQQLFNVE
                     RSTLLSHHKFNPKISNNREFLQDLEARYDRLNQAIENNEPYPFLYGDVCLLKEYLQVI
                     LGYYQDQLKAHQPVANSYLKRITKSHKFSTLMSDISEEEHPELGKKDSEILIKYTINF
                     CAKKIMMEDLKTISDLVIKPFLFDHKDEEGFSYCNP"
     gene            3730..4425
                     /locus_tag="I9760_001634"
     CDS             3730..4425
                     /locus_tag="I9760_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT5043405.1"
                     /translation="MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLV
                     QIKYEGENFNDIFLGLANKKYDVVISGTTITPNRSKQVLFSNPYLQFNQGIAVNTELK
                     PNVSSFEDLTGLVAGIQKGNTSDEVAQDLLRKNILSDIKYYAYNEIELAIQDLSAGKI
                     GLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAFAKDNFKLRDKVNIVLADLVQNG
                     TLSRLQHKWISTT"
     gene            4738..8169
                     /locus_tag="I9760_001635"
     CDS             4738..8169
                     /locus_tag="I9760_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HAT5043406.1"
                     /translation="MKILKTTEKKSRKPTPKENRALPLQLVQNTEPDRTYYLRSTVEG
                     IGSFLSTRVRELYNTLAMTFSLDSMDWSSFKKYIWPTISSGAALLLGDYLTRSMSEYQ
                     TGSPEYDFIFKFLFGTAILGYCALNRITDRKFTSQVELSAAVASEVMAITYMYSSVFK
                     GTTYLLNDSISDEYSLIAGLGTSALLVPSGISYLTQKIQEFLVRIQYNKGAQRSDTAV
                     ISSGLTPISNLYFQINHFIASELMISSRAFQLGLISHNIRYADRIMQKFRNLPALLAD
                     LAPSTIKKMRIQQQKLEHDYEYNHKLHQVLRFTADGPMFISIPRYQLRTGDLVYCDSS
                     IDLACVPVSGELLALRRDLKGEFTQTLEQKKFSVNLKAQNGEDVWIEHETKPDFTSHY
                     KKVDLHAVRDGKQAGVLVGDKLNLYGNDNIFIQIKPEKELLLSSNYEKKAVINEIIAE
                     RKQRSVLYSILGSVIMAAFLQRDITAMPAETLRLMFTLFQTMIPFSEAFLRETVNSRL
                     MKKLNSELGDQPFGTIDALRVVDLCNALGGYYRDRFPNGVAIISDKTGTLTTTRMDVL
                     GLWTNDMAPEVQKTLKEKEGLLLPEETQWLQTFEVFCNAFTNNKKELEPEEHAILELF
                     QSLLDNRQCLEVITLGNNHFKKEMISKDIKKEIETFHLGLYRTFGGRFTLVQDGSDHY
                     LVFCGVPKQEAFKETPLLKAYTTMQSRTGVLSRDWCLARGKLSEREFTILKELFSKDD
                     KKQIEHFILQNKLLLENLMYYGTFIIDNPVKKGAEKFIAQCREISVPVFVATGDTTKA
                     AENIAKVLCPANAGKIVTLRADQIDEKGIEFLNEENYPSDSTVIFSGINEAILNRFQK
                     LMGREGAKRPVVIFAEMSTEGKGVLARYLKDHHYFVVANGDGTNDVMMMKNSNVVIAH
                     HSDDGTFAPGVDGLTNISEEQLRRLFGSQKTFYELFDINLPKSLFVQKFAPLANSQEK
                     PTMALALKSGKMTFDLAKTVGADVTEMNQQHWYSVVFDLLWLWIAFYEINESSDLPMD
                     NRNINASRLISNTMGIALIIAVFQSLANYALFNESTNLTSMLIMLSFLPLILKSIFSG
                     FRMVQDSLYPQVEVTEVEEEGDTSTNGSVLSYFGTFFSRKPVKQLEGLNCAPALK"
     gene            complement(8265..9773)
                     /locus_tag="I9760_001636"
     CDS             complement(8265..9773)
                     /locus_tag="I9760_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HAT5043407.1"
                     /translation="MTKKRIDRLIFSEVECNMKKCSMVIAGKPISYEKLFGTVNRIAI
                     HFQNKALSGSRIAFMLPNSLEIIAIYLACFQSGCIAMPVNRRYAPPELEKVLRDAQPI
                     YLIIEASKLFLLEKINWSATGIKKIFVLGENQNYPYFSFNELLNEPANCPKNGIDYKA
                     PAVIFYTSGSTGEPKGVVHTLSSIEAMLDSTSLALENITADDKMIICEPQCHVSGFIE
                     TFSTLSQEGTALVHDGFDMDWYLATVRHEKPTLAVHHIDTYIKLLDSGRCNTKTFQSF
                     RGIYTGGDTLPVAVQDKFLACAGKPIQVGYGMTEAIWLTVCRTPDLTQKSCIGKPVNG
                     VTLRLVNAAGEDVRCGETGEILVKGDMVMHSYWNNPEETRKAFIKGWFKTGDCGIQDE
                     QGYFYYKGRIKNIIIRNTSNIMPGEVEATIYKHPAISAAAVIGVPDEAEGEVPIAFVV
                     VKKSNQLTKEELYSFLIEQIAQYKIPAKIYFIDEMPLTNSGKINHKKLYDYL"
     gene            9856..10320
                     /locus_tag="I9760_001637"
     CDS             9856..10320
                     /locus_tag="I9760_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HAT5043408.1"
                     /translation="MDFIKRTIELARENVGHGGRPFACLIVQNGKILAEATNTVAQTH
                     DPTAHAEVNAIRIATAKLGCEHLYDCDFYILAHPCPMCLAAMYYCSPRKVIFITTRDE
                     YKTYYVDDRKYFTLENFYQEINKPWRERKMPMEHRPDKEAIEVYKMHQKKKS"
     gene            10748..12307
                     /gene="ravO"
                     /locus_tag="I9760_001638"
     CDS             10748..12307
                     /gene="ravO"
                     /locus_tag="I9760_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavO"
                     /protein_id="HAT5043409.1"
                     /translation="MPTGIVLSSQSPEATTSFPRRGGTAAGINHVTGFPVIKMHRDAQ
                     ARPDERKYFNQIKDGSLVFITGHSNTALDHLTGDYVPPLATPVQPMSTEWTYKQFRDL
                     ILKNGSLKAGDTITVVLWACNAGEGGLSSGAGLLGRLFREKQINTRVIASAATTMRFD
                     GHYSPTPEGTPAMAFITENGSKDIRIFDFTEEATIEWKNKGRLYVTCNGIEGYNLYSP
                     KQLESLESEKIYKVMEEIFVDRHYKENIRTGEEVKQYFSKSKAEFILRWSSANESDTE
                     NKYVFIAASFQASDGIHSIRYGINKNGELFSINTASNKVTPIDILPLGVMATLTQHIT
                     QNKELIEKALHEKGAQMTPSPKLQHRVKRKKSDHTELLILKGEKEPLKPVQTDTLSSK
                     VPASPGWSTQENQDFLMFVTPLQVQELLKSHGETKKEKHEKKIRLGHEKTIGDEKTRK
                     PVSKHKEPSGENEEPEMDLSKRQRIPVVQRKRPQGRRHEFFSDSPKNPDEHKTEKDKT
                     ISPSRNPTKGG"
     gene            complement(12436..13887)
                     /locus_tag="I9760_001639"
     CDS             complement(12436..13887)
                     /locus_tag="I9760_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HAT5043410.1"
                     /translation="MKFRTKLFLGLLLIVITTNGIFLFLSYQYGKETVYKEVGSSALS
                     IAAATAVLMNPEDIQKFNLDTSANSPLYKDLEKKLLEVRNANRRKDVYVNFIYSLFPD
                     SNNPKVLRFAVDPEENTIDKTELGTVMKLKTESGQTINVNYESPIVLPEFVEDIWGTW
                     LTALYPIKDAQGKYLGNIRVDVDADSVNQRFHFLLYSGIAVFISTFLFAIFLGWLLFQ
                     WFNKPLLKITTALKNIAQGDLEQHLDIRTKDEFAEVGRVINEMTEGLRQRNMLQVSLT
                     RFISHALAEKIVKSGELPQVFSERRKVTIMVCDIRDFTTISERIKPELVVDFLNHFFE
                     KMIEAITSQHGILDKYLGDGFLAIFGSPDDDAYQEDHAIHAALKMREAMKSLNADWAK
                     ILGSEIQIGIGINTGSAIVGNIGTDIHMEYTAIGDTVNLASRIENATKKLNTDILISE
                     YTYIATDHTAFQFIELGEISIKGRVHKVKVYTL"
     gene            complement(14049..14375)
                     /locus_tag="I9760_001640"
     CDS             complement(14049..14375)
                     /locus_tag="I9760_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043411.1"
                     /translation="MPKNNEPSGEELKKAGIVVGITNLLLWPFYYADSRWGLFASIFA
                     TSAVLYASHEVGESKRPVENSVARLGLFQQRGASNAEIENAFNNTVTGAAAIYDWLVP
                     GSNGPK"
     gene            complement(14540..>15322)
                     /gene="cfa"
                     /locus_tag="I9760_001641"
     CDS             complement(14540..>15322)
                     /gene="cfa"
                     /locus_tag="I9760_001641"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAT5043412.1"
                     /translation="NDLFQIMLDSNMNYTCGYWRKAENLEQAQLDKLDLTCRKLCLKP
                     GMKLLDIGCGWGGLAKYAAENYGVSVVGITISQQQYELAKTRCAHLPVEIRFQDYRDL
                     NEKFDRIVSLGMFEHVGYKNYRKYMEIVHQCLNDDGLFILHTIGSNESVTKATPWISK
                     YIFPNGMIPSIMQIGQASEKLFVMEDWHNFGADYYKTLMAWHENFNKGWDQIKSQYSE
                     KFFRMWNYYLLSCAGAFDARMLQLWQIVFSKKGIQGGYSAPR"
BASE COUNT         4844 a         2696 c         2932 g         4850 t
ORIGIN      
        1 atggccaggt tctggttaat tctcggaaaa tgctttggag tttgcatttc gtgcaaaatg
       61 caatgctaaa tgtctaccca ccaaccttga tgcttgatac acacaaccgg ccatcccatt
      121 tccatatgaa aataaaccct aaccgcgctg ataaaaatca gtttgtcata aataattgct
      181 ggaggggcca tcagcatata ttgaaacaaa tgttagagca atattaataa aaaaacgtta
      241 aatagacagg gtgttaaatt atcatggcct tatattgcag gaagtgcttt atacaagata
      301 ctaagaggag tatattttat aagacgcgct caggaccggt tcaggcgaag agaaacaata
      361 cattataaac gggctccaga gaagctttat agagcaggaa tagtaatttg attaagacat
      421 tcatcgataa aaaaatacag ctgctattca gttgccttat tttttttatc ttcagtactc
      481 atgctaaatc atttgaaaca cctgatgtat gggatgtttt gcgcagtgaa ttcaagatga
      541 atcatgaagc ctatcgccca gaggttcaag atcaaattcg ctggctactg gatcatccag
      601 ggtttgtgac taaggcctgt aaacaggctg agccttatct ttatcatgta actcgagaaa
      661 tacaaagaag aaatttaccc ggtgagttgg cattactccc tatgatagaa agcgcttacg
      721 atccttttgc ttactcaaag gttggtgctg caggtttatg gcaaataatg ccggtgacgg
      781 ggaatcaatt aggtttaatt caggattggt ggtttgatgg aagacgaagc attaatcatt
      841 caactgatgc agccctgact cacctgatgc atttaaataa agtgtttaac ggaagctgga
      901 tactttccat cgcagcttac gatgctggcg aaggagccat aggcagagct gtaaaagcaa
      961 ataacactaa cggaggtagc gtcagttttt ggaatctgga tgtacctaaa gaaactcaaa
     1021 tttatgtccc tcgattttta gctttggctg aaatcatgag caaccctcgg gcttataaaa
     1081 taaatcttcc tgcaacgcca tttcgacctt attttgaaga ggtgaatatt ggcagtcaaa
     1141 ttgacttgaa tcatgcggct agtttagcag gaatttcggt tagtgaatta accaaattaa
     1201 atccgggtta taatagatgg gctaccgcac cctataaacc cttcactttg cttatcccta
     1261 tagcaaaagt gactcaattt tatttcaatt tatcccattc acctgttgat aaacgagtga
     1321 gctggagaaa acatctcgtc cttccaagtg atagtttaca aagtattgcc agaaaatatc
     1381 atacgactgt ttcattaata aaaaaattaa atcgtctgtc agaacactcg ataagaccca
     1441 atcagatctt gttaattccg agcgccaaaa gtactccagc attgcctttc cacgaaattt
     1501 caaagtcaaa attcttaagc cagacaccaa caaaaggtaa agtatacagg gtcatacata
     1561 ttgtccaagc caatgaaacg tataatactc ttgagcgact ttataaagtt acagctcagg
     1621 atattatgaa atggaatcat attggctcgg gagaagcgtt ggtaaaaggg cgacagttaa
     1681 ttatttggaa aaagtcaagc aatcttgaat ggaacaggac tttcaaaaaa acttaagttt
     1741 ccggcaatcc tgcattctaa acataacccg tgttcagtca aagtttcacg cttgcctatt
     1801 cgataactta ttaataaatt tggttaaatt tctcatatgt ggcaattttc atactaacct
     1861 actaatgatg aagattatat gctggatttg caccaaaata ttcgttgtag atacggaggt
     1921 aggaaccatc tttttccatt tccagcaaaa tgtgattaat tttatcaatc aatgccgatt
     1981 ggtttttaag ggctatgatc ccatatccgc ttcctatcgg tattttatcg ccaatcaatt
     2041 ttaattgatc gtcttcagtg gggttgagat tattcacgat gaatcgtgac gcttgataat
     2101 tgattaaaat cgcatcaatt tttttggttg ttagtgcatt aactaattct gccggatctg
     2161 tgtactctat aagcgtattg gtggtttcgt atttttttaa aagaggagat tgctgaaaat
     2221 taatcttaaa gaaaccaact tttttaaaat tgatatcgtt caaggtatca atcgagttgt
     2281 tttttaatgt cagaaattga ccgtcactgg gtaagtaggg caagctgtac cgatagttag
     2341 caggtaatga tgtcgtgata ggggttgagc taaaaacaat atgataaaca cctttatcca
     2401 gaccttttaa ctcgccttct ttaggaatag ggatgtattc gcatttttct tgcaaacggt
     2461 tgcatattat attcatgagg gcaacaacga aaccataata gtgatttgct ttgtctgata
     2521 tagatgagaa aggaggagca aattgtaaga cacccacctt gagtgattca ctgtgaacta
     2581 gaacgttgct tataaacaac agtataaata ctatccgttt cataaattgg gtctcttctt
     2641 aatctggtcc atttttaata agagaagtaa tcttttttaa ttatagggca atagttttca
     2701 ttttggatcg gtttttcaga gtcaatttta gtgaaataaa atattgttag ctgcgcaatc
     2761 aagaggcttt tcaaggtagg gcttggaagg ggttgggcag tatccgattt agagatatcg
     2821 ttaaatccag gtcactgccc tctaatctat tctccagctt atggattgca ataagaaaaa
     2881 ccttcttcgt ctttgtgatc aaataaaaat ggttttatta caaggtcact aattgttttt
     2941 agatcttcca tcatgatttt ttttgcacag aagttaatgg tgtacttgat taaaatttcc
     3001 gaatcttttt tgccaagctc aggatgctcc tcttctgaaa tatcagacat tagagttgaa
     3061 aatttgtggc ttttagttat ccgttttaaa tagctgtttg ctactggctg atgtgcttta
     3121 agctgatcct ggtaatagcc tagaatgact tggaggtatt ctttcaataa acatacatcg
     3181 ccgtataaaa aagggtaagg ttcattattt tctattgcct gattgaggcg atcataacga
     3241 gcttccagat cttgaaggaa ttctcgattg ttacttattt tggggttgaa cttgtgatga
     3301 gaaagaagag tacttctttc aacattaaac aattgttgaa aattaaagat aatctttgaa
     3361 atttttttaa gataatctgt agtacataat atttcaaagg cttcctggct ggttaatgga
     3421 attgttttag aaaacatttt catacgcatg acgtgatctc tggttaattt agatttaatt
     3481 taaatctaaa tgattaaaat tcagcattat ataacaatgc attagataga taaataaaca
     3541 tagtgatttt tttatattct ttttggtttt tataatttta gctgttaatt acataaccaa
     3601 tcctggatat attactcgtg taatcatggg taaattattc atttaaatca atggcataaa
     3661 aaatatttgg ctgaattttt tctgattaat ctatacttag tgaatcgatt ccaaacaata
     3721 gttatatcta tgattctaaa gccgggaaaa ttattggtgg ctactgctta tccagatcca
     3781 ccgtttgata ttgagccagg tgatggtttt gatggtgatt taataaaaga aatttgtcga
     3841 attttaaatt tgcagctagt ccagatcaaa tatgagggag aaaattttaa tgatattttt
     3901 ttaggcttgg ctaataaaaa atacgatgtt gtcatttcag gaacgactat tacaccaaac
     3961 cgctcaaaac aagtactttt ttcgaatccc tatttacagt ttaatcaggg tatcgcggtt
     4021 aatactgaat taaagccaaa tgtttcttct tttgaggatt tgacaggttt ggtggctggt
     4081 atacagaaag gcaatacctc ggacgaagtt gctcaggatt tattacgtaa gaacatactc
     4141 agtgatatta aatattatgc atacaatgaa atagaattag ccatacaaga tctgtctgcc
     4201 ggtaaaattg gtttggtcat taaattatat cctgttattt cctgtttaat tcgaaactat
     4261 acaaatttgg ctgttccttt gcaggctgaa acacatgaaa aacttggtat cgcgtttgct
     4321 aaagataatt ttaaactgcg ggataaagtc aatattgtcc tagccgatct tgtgcaaaat
     4381 ggcactttaa gtcgcttgca acataagtgg ataagtacaa cctagggtaa attaggccaa
     4441 tgtcagtttc aactcaggag ataaccttat ttaaagacag gacttacgat aaactccaat
     4501 ctctttgtta aaaaaagttt ttaactcggt catttagttt atctaaactc cctcattaaa
     4561 aacatttttt gcctcgacct tggagttttt cgtaagtcct gaaagaatct tatttttctt
     4621 gaatcgttct gtaattattt ttcaaatcct cttgaacttt tatacagata agttaattat
     4681 caggttgagc tctaaagatc caatagagtg ttattggagt acaaaaggat ttataacatg
     4741 aagatattaa agacgaccga aaaaaaatcg cgaaaaccta ctcctaaaga gaacagagct
     4801 ttaccgcttc aattggtaca aaatactgag ccggatcgaa cgtattatct gagaagtact
     4861 gtagaaggca tagggagttt tctttctact cgtgtaaggg aactttataa cactttagcc
     4921 atgacttttt ctttagactc tatggattgg agcagtttca aaaaatacat ttggccaacg
     4981 atatcctctg gagcagcatt gctgcttggt gattatctaa cccgatccat gagcgaatat
     5041 caaacgggaa gcccggaata tgattttatt tttaaatttc tttttggtac agcgatatta
     5101 gggtactgtg ctttaaatcg gataacggac aggaaattta cttcccaggt tgaattatct
     5161 gctgcagtag ccagtgaagt catggcaata acctacatgt atagttcggt tttcaaaggc
     5221 accacttatt tattgaatga tagtatttct gatgaatatt cattaattgc cgggctagga
     5281 accagcgcgc tgctggttcc tagcggaatt tcttatctaa cacaaaaaat acaagagttt
     5341 ttagtgcgaa tccaatacaa caagggtgcg caacgctcgg atacagcagt gatttcaagc
     5401 gggctcacac ccatatccaa tctttatttt caaatcaatc attttattgc cagcgaactg
     5461 atgatcagtt cacgcgcatt tcagctgggg ctcatttcac acaacattcg ctatgcggat
     5521 agaataatgc agaaatttcg taacttgcct gcattattag cggatttggc accatcgacg
     5581 ataaaaaaaa tgcgtatcca gcaacagaag cttgagcatg attacgaata caatcataag
     5641 ctacatcagg ttttaagatt tacagcagat ggcccaatgt ttataagtat cccaaggtat
     5701 caattacgca caggggatct ggtttattgt gatagcagca tcgatttggc ttgtgtaccg
     5761 gtttctgggg agctgcttgc tttaaggcgt gatttaaaag gagaatttac tcaaaccctg
     5821 gaacaaaaaa aatttagcgt taacctgaaa gcccaaaatg gggaagatgt ttggatagaa
     5881 catgaaacca agcctgattt tacctcgcat tataaaaagg tggatttgca cgctgttaga
     5941 gatggcaaac aggcaggagt attggtaggc gataaattaa atttatatgg taatgataat
     6001 atttttattc aaatcaaacc cgagaaagag cttttgttaa gcagtaacta tgaaaaaaaa
     6061 gcagtcatta atgagattat tgccgaacga aaacaaagaa gtgttttata ttctatttta
     6121 ggctccgtta tcatggctgc ttttttacaa agggatatta ctgcaatgcc tgcagaaacc
     6181 ctaaggctca tgttcacact ctttcagact atgattcctt tttctgaagc ttttttacga
     6241 gaaactgtga atagccgttt gatgaaaaaa ttgaatagcg agctcgggga tcagcctttt
     6301 ggaaccatag atgctcttcg tgtggttgat ttatgtaatg ctcttggcgg ttattatcgt
     6361 gacagatttc ccaatggtgt tgccattatt tcagataaaa ccggaactct tacaactaca
     6421 agaatggatg tattagggtt atggactaat gatatggctc ctgaggttca gaagacatta
     6481 aaggaaaagg agggtttgct cctgccagaa gaaacccaat ggctgcaaac ttttgaagtg
     6541 ttttgcaatg cgtttactaa caataaaaaa gaattggaac ctgaggaaca tgctattttg
     6601 gagttatttc aatccttact cgataatcgg cagtgtctgg aagtgattac tttagggaat
     6661 aatcatttta aaaaagaaat gatctctaaa gacattaaga aagaaattga aacgtttcat
     6721 ttgggtttat atcgtacgtt tggcgggcgc tttactttgg ttcaggatgg ttctgatcat
     6781 tatctggtct tttgcggagt gcccaaacaa gaggctttta aggagactcc tttgctaaaa
     6841 gcctatacga ctatgcaaag tcgtactgga gtgttatctc gtgattggtg tttggctcga
     6901 ggtaaactca gtgaacgcga gtttactatt ttgaaagaat tatttagcaa ggatgataaa
     6961 aagcagattg agcattttat tttgcaaaat aaattattgc tcgaaaattt gatgtattac
     7021 ggtactttta ttattgataa tcctgtaaaa aaaggagcag aaaaatttat tgcccaatgt
     7081 cgggaaatat cagttccagt ttttgtagca acaggagata cgaccaaggc agcagaaaat
     7141 attgctaaag tattgtgccc ggctaatgca ggaaaaattg ttacgctgcg tgccgatcag
     7201 attgacgaaa aaggtattga atttttaaat gaagaaaatt atccctctga ctctacagtt
     7261 atattttcag gtattaatga agcgatattg aacagattcc aaaaactgat gggtagagaa
     7321 ggggcaaaac gccccgtcgt tatttttgct gaaatgagta ctgagggaaa aggtgtttta
     7381 gctcgttatc ttaaggacca tcactatttt gttgttgcca acggggatgg tactaatgat
     7441 gttatgatga tgaaaaattc taatgtggtg atagcgcatc attcagatga tggaactttt
     7501 gctccaggag tggatggctt aaccaatatt agtgaggagc agctaagacg gttgtttggt
     7561 tcccagaaaa ctttttatga actgtttgac attaatctac ccaaaagcct gttcgttcaa
     7621 aaatttgccc ctctagccaa ctcacaagaa aagccaacca tggctttggc actcaaaagt
     7681 ggaaaaatga cgtttgattt ggctaaaaca gttggagcag atgtcacaga aatgaatcag
     7741 cagcattggt atagcgttgt ttttgactta ttatggttgt ggattgcttt ttatgaaata
     7801 aatgaaagct ccgatttgcc aatggataat cggaacatca atgcttcaag attgatttcc
     7861 aatacaatgg ggattgcctt aataatcgct gtttttcaat cccttgctaa ttatgctctg
     7921 tttaatgaat ctacgaactt gaccagtatg ctgattatgt tgagtttttt gcctctaata
     7981 ctaaagagta ttttctcagg ttttagaatg gttcaggaca gcttgtaccc tcaggtggaa
     8041 gtaaccgaag ttgaggaaga aggagataca agtactaatg gatctgtcct aagctatttt
     8101 ggaacttttt tttcacgcaa gccagtaaag caattggagg gtttaaactg tgctcccgca
     8161 ttaaaatagc cccttgcata aaggggctgt ttttatttta tcgctcggtc ataaatattt
     8221 ttgtgatgct tattgacctc atgtaaactg tgtatttttt aattttaaag ataatcatac
     8281 agtttcttat gattaatttt tccactattg gtcaagggca tctcatcaat aaagtatatt
     8341 tttgcaggta ttttatattg agcaatttgc tcaattaaaa aagaatataa ttcttctttt
     8401 gtcaattggt tactcttttt tactactaca aaagcaattg gtacttctcc ttctgcttcg
     8461 tcaggtacac caatcacagc cgctgcgcta attgctggat gtttataaat ggttgcttcc
     8521 acttcaccag gcataatatt ggatgtattg cggataataa tgtttttaat ccgaccttta
     8581 taataaaaat aaccttgttc atcctgaatt ccgcaatctc ctgttttaaa ccaacctttt
     8641 ataaatgcct tgcgggtttc ttcaggatta ttccaataac tatgcatcac catatcacct
     8701 tttaccagta tttctcctgt ttcaccacaa cgtacgtctt ctccagctgc gtttacaagg
     8761 cgtaatgtta ccccattcac cggtttgcca atacaggatt tttgagtcaa atcaggggtt
     8821 ctgcagactg ttagccaaat ggcttccgtc attccataac ccacttgaat tggtttacca
     8881 gcgcaagcca gaaacttgtc ctggactgcc acaggtagtg tatccccacc tgtataaatt
     8941 ccacgaaatg attgaaatgt ttttgtatta cagcgtccag agtctaataa ctttatatag
     9001 gtatcaatat gatgaacggc cagtgttggt ttttcatgtc gcactgtcgc aaggtaccaa
     9061 tccatatcaa aaccatcatg aactaacgcg gtaccttctt gtgataatgt agagaatgtt
     9121 tcgataaatc cacttacatg gcattgtggc tcacagatta tcattttgtc gtcagcagta
     9181 atgttttcca gtgccaatga agtcgagtca agcatcgctt caatggaaga aagcgtatga
     9241 acaactcctt tgggttcccc cgttgaaccg gaggtataaa aaattactgc aggcgcttta
     9301 taatctatac cattttttgg gcaattcgct ggttcattta ataattcatt aaaagaaaaa
     9361 taaggataat tctgattttc ccccaggaca aaaatttttt taattcctgt cgctgaccaa
     9421 ttgattttct ctagcaaaaa taatttactt gcttcgataa ttaaataaat tggttgggca
     9481 tcgcgcaata ccttttcaag ctcaggtggc gcatagcggc gattaacagg catggcaata
     9541 catcctgatt gaaaacaggc caggtaaatg gctataattt caagactatt gggcaacata
     9601 aaagcgatac ggcttccaga gagtgcttta ttttgaaagt gaattgcaat acgattcact
     9661 gtaccaaaca atttctcata ggatatgggt ttgcctgcaa taaccataga gcattttttc
     9721 atattgcatt caacttctga aaaaataagc ctgtctattc tttttttagt cattatgttt
     9781 tcctggatag attcatttgt tactttttat attatgggaa ataattaata attatgggct
     9841 aaaaatggga aaaaaatgga ttttataaag cgtactatcg agctggctcg agagaatgtg
     9901 ggacatgggg gcagaccatt tgcttgtctt atagttcaga atggaaaaat tcttgctgaa
     9961 gctaccaata cggtggcgca aacccatgat cctacagccc atgctgaagt taatgcaata
    10021 cggattgcta cagcgaaatt aggttgtgag catttatacg attgcgattt ttatatactt
    10081 gctcatcctt gcccgatgtg cctggccgca atgtattatt gtagtcccag gaaagttatc
    10141 tttatcacga cgagagatga atataaaacc tattatgtag atgatcgaaa atacttcact
    10201 cttgagaatt tttatcagga gatcaataag ccatggagag aacgtaagat gcctatggag
    10261 catagaccgg acaaagaggc aatagaagta tataaaatgc accagaaaaa aaaatcttag
    10321 tccaaataac tgtttaatat ttaaaacagt ttgattttca acctttaaaa aacttttatg
    10381 ttttaaagtt catagtcttg cttgatcact ttcaataaat catgtttatc ttgagtagta
    10441 tcttcctgct tttttcagtc taccttttga gcatatctta aaaattttaa aacatctcaa
    10501 atgtattttt tatttcaatg ctgattgtta ttaatataat cttaatgttt ttataataaa
    10561 ctatgctggt tttatattag acagcacgaa gttaatagaa agtttccttc aaaatcaaga
    10621 tgattcccgt tgatcattat aaagcactcg caattgagca aagcaaaaca actttgaagt
    10681 tccaataaaa atctgattgc atataatttt gctcaaattt tcaagataaa ctaattgagt
    10741 atgaataatg cctactggaa tcgtattaag ttcacaatcc cctgaagcca caacttcttt
    10801 tcctcgtcga ggaggaactg ctgctggaat aaaccatgtg actggttttc ctgttattaa
    10861 aatgcataga gacgctcagg ctcgtcctga cgagcgaaag tactttaatc aaattaaaga
    10921 tggcagcctg gttttcatta ccggacatag caatactgcc ttggatcatc ttactggcga
    10981 ttatgttccc ccattagcga ctccggttca gccaatgagt accgaatgga catataaaca
    11041 gtttagagat ctgattctga agaatggaag cctcaaagca ggtgatacga taactgtcgt
    11101 tctttgggca tgtaatgcag gtgagggagg tcttagtagt ggggcaggct tattggggcg
    11161 cttattcagg gaaaaacaaa ttaatacaag agttattgct tcagcagcaa ctactatgcg
    11221 atttgatggt cactattctc caactccaga gggaacgcca gctatggcgt ttataacaga
    11281 gaatggtagc aaagatattc gtatttttga ttttactgag gaagcaacga ttgaatggaa
    11341 aaataaaggg cgtctttatg ttacctgtaa tgggattgaa ggctataact tgtacagtcc
    11401 taaacaatta gaatccctgg aaagtgaaaa aatatataaa gtgatggagg aaatatttgt
    11461 tgacaggcac tacaaggaaa atatcagaac tggagaagaa gtgaaacaat acttttccaa
    11521 gtctaaggca gagtttatat tacgttggag ttcagccaat gaatctgata ctgaaaataa
    11581 gtatgtcttc atcgcggcat cttttcaagc ctctgatgga attcatagca ttcgatatgg
    11641 aataaacaag aatggagagc ttttttcaat aaatacggct agcaataaag ttacgccaat
    11701 tgatatttta ccactgggtg taatggctac tttgacgcaa catattactc aaaataaaga
    11761 gttgatagag aaagcactcc atgaaaaagg agctcaaatg actcctagtc caaaactaca
    11821 acatagagtg aagcgtaaaa aatcagatca tactgaattg cttattttaa aaggcgagaa
    11881 agaaccatta aaacccgttc aaacagatac tctctcaagc aaggtgcctg cttctccagg
    11941 atggtcaacc caagaaaatc aggatttctt aatgtttgtt acaccgttgc aagtccagga
    12001 attgcttaaa tctcatggag aaacaaaaaa ggaaaaacat gagaaaaaaa tcagattagg
    12061 tcatgaaaaa actattggtg atgagaaaac aagaaagcca gtatctaaac ataaagaacc
    12121 atctggagaa aatgaagaac cagaaatgga tttaagtaaa aggcaacgaa tacctgttgt
    12181 acagagaaaa cgacctcagg gtcgtaggca cgagttcttt agtgattccc caaaaaatcc
    12241 agatgagcat aaaacagaaa aggataaaac gatatctccc tcgcgaaatc caaccaaagg
    12301 aggctagctg gcaagcaaag tgttgttggt gttgattaag actataccat tttgacacaa
    12361 gaattagtgg gtgtattttg atatggtgtg ataaagggtt cccagtcaat tgttacagat
    12421 taaaaataat taatcttaaa gcgtataaac ttttacttta tgaactcttc ctttgataga
    12481 aatttctccc aattctataa attgaaatgc cgtatgatct gtcgcaatat aagtatattc
    12541 actgattaga atatctgtat tgagtttttt ggtggcattt tctattcgtg atgccaaatt
    12601 gacagtatca ccaatagctg tgtattccat atgtatatca gttccaatat tcccgacaat
    12661 ggcactgcct gtattgatac caataccaat ttgtatttca gagcctaata tttttgccca
    12721 atcggcattg agtgatttca tcgcttctcg catttttaat gcggcatgga tagcatggtc
    12781 ttcttgatag gcgtcatcat caggtgaacc gaaaatcgct aaaaaaccat ctccaagata
    12841 tttatccaat attccatgtt gcgaggtaat agcttctatc attttctcaa aaaaatgatt
    12901 taaaaaatca actaccaatt caggttttat acgctccgaa atggttgtga aatcgcgtat
    12961 atcacagacc ataattgtga ccttacgtct ctcgctaaat acttgcggta actcaccaga
    13021 tttcacaatt ttttctgcta aagcatgaga aataaaacgt gttaagctta cttgcagcat
    13081 gttacgctgt cttaatcctt cagtcatttc attgattact cgacccactt ccgcgaattc
    13141 atcttttgtc ctaatgtcga gatgctgttc aagatcacct tgggcaatat ttttgagtgc
    13201 agttgtaatt tttaataagg gtttattgaa ccactgaaaa agtaaccagc ctaaaaaaat
    13261 ggcaaataaa aatgttgaga taaagacggc aatacctgag tacagcaaga aatgaaatct
    13321 ttggttaacc gaatcggcgt ctacgtccac tcggatattt ccaaggtatt tcccttgtgc
    13381 gtcttttatt gggtataaag cagtcagcca tgtaccccag atgtcttcaa caaattcagg
    13441 cagaacgata ggggattcat aatttacatt aattgtctgc cctgattcag tcttaagttt
    13501 catgacagtt cccaattcag ttttatctat agtgttttct tcgggatcta cggcaaagcg
    13561 aagtaccttg ggattattag agtcaggaaa taaactatat atgaaattca catacacatc
    13621 tttgcgacga tttgcgttac gtacttcaag aagttttttt tcaagatctt tgtaaagagg
    13681 tgagtttgca gacgtatcca gattaaattt ctggatatcc tccggattca tcaggacagc
    13741 tgttgccgca gctatggata gggctgatga gcctacttct ttgtatacgg tttctttacc
    13801 atattgataa gataaaaaca gaaatatgcc gttagtagtg attacaatga gaagtaggcc
    13861 aagaaataac ttagtacgaa atttcattat ttatccacca ttagcagatt aattataagt
    13921 attggataaa ggcagtgaga agtaaagaca acagatttct ggagatcttt accaatctcc
    13981 agaacgatgt ggttttggat aaaataatac ttcccaaaaa tataatttat tataagacag
    14041 gatgcttatt atttggggcc attagagcca ggaacaagcc aatcatatat cgcagcggca
    14101 cctgtgaccg tattattaaa agcattttca atttcagcat tgctggcccc acgctgctgg
    14161 aagaggccaa gtcgcgccac tgaattttcg acgggccgtt tactttctcc gacttcgtga
    14221 gaagcataaa gtactgcgga agtagcgaat atggatgcaa ataaccccca tctcgagtcg
    14281 gcataataaa atggccataa aagcaggttt gttattccta ccactatccc tgcttttttg
    14341 agctcttctc cacttggttc attatttttt ggcatattaa ttccttttta gacaaaattt
    14401 cagttcaaac tatattccag ataataaaaa ttgcaagcaa aattaacggg atatatgaat
    14461 aatcttgcca acttcatggc atggaaaacc aggtttgctg gtgaaggtat tacgatacgg
    14521 aataaatcgt ttaaaccatt tacctcggcg cagagtaacc tccttgaatt cctttctttg
    14581 aaaaaacaat ctgccataat tgcaacattc tggcatcaaa agcgccagca caagagagca
    14641 aataataatt ccacatacga aagaattttt cgctatattg ggatttaatt tggtcccatc
    14701 ccttattgaa attctcatgc catgccatta atgttttgta ataatcagct ccaaaattat
    14761 gccaatcttc cataacgaat aatttttcgg aagcttggcc tatttgcatg atggatggaa
    14821 tcattccatt gggaaaaata tattttgaaa tccagggtgt ggcttttgtg acactttcat
    14881 tggaacctat agtgtgtaat atgaacaaac catcatcatt taaacattga tggacaatct
    14941 ccatgtattt tcggtaattc ttgtaaccaa catgctcaaa catccctaag gagactatac
    15001 gatcgaattt ttcatttaag tctctataat cctgaaatcg gatttcaaca ggcaaatgag
    15061 cacagcgcgt tttagctaac tcgtattgtt gctgtgatat cgttatgcca actactgata
    15121 ccccatagtt ttcagcagca tattttgcaa gtcctcccca tccacatccg atatccagca
    15181 atttcatacc gggtttaagg cagagttttc tgcaggtcag atcgagcttg tcgagttggg
    15241 cttgctccaa attttcagct tttctccaat aaccgcaagt gtagttcatg ttgctatcaa
    15301 gcataatctg aaacagatca tt
//
LOCUS       DACTJE010000069        15243 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.069, whole genome shotgun sequence.
ACCESSION   DACTJE010000069 DACTJE010000000
VERSION     DACTJE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15243
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.069"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            73..1590
                     /locus_tag="I9760_001642"
     CDS             73..1590
                     /locus_tag="I9760_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAT5043413.1"
                     /translation="MKHGLFAHLKHDLPASIVVFFVALPLCLGIALASHVPLFSGIIA
                     GVIGGVVVGFISGSALGVSGPAAGLVAIVISSVETLGSWEAFLLAGVIAGILQLIAGF
                     LRGGIIAYYFPSSVIKGMLTGIGIIIVLKQIPHLLGHDVTPLLLSMSDGELDINDIRE
                     ALDDINPEVVIISIVSLLTLIFWEKLVKKHKLFEMIQGPLVVVILGIALNLFYEHRLT
                     SFSLDPSELVQLPKFNKASELFNYLTLPDFSLITHLKIYKIAILIALIASLETLLCVE
                     AVDKLDPYKRVTSTDRELKAQGVGNILSCLIGGLPITQVIVRSSANVAFGAKSKLSAI
                     LHGLLLFICVISIPEILNEIPLASLASILIIIGYKLAKPALFKQMYKMGWEQFVPFMI
                     TIIGIIFSDLLFGITLGFSLAIFIILRHHFLNSHDMIKTRGKNKTQYCLKLAEEVTFL
                     NKGSIINELKNIPEGAEVVIDGSRSKIIDHDIKEVIQNFILNSKTKNIQVKLIGI"
     gene            1594..2220
                     /locus_tag="I9760_001643"
     CDS             1594..2220
                     /locus_tag="I9760_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842179.1"
                     /note="macrophage inducible 5; Mig-5; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HAT5043414.1"
                     /translation="MWTLTKEQQQAITPEKAIELLKEGNKRFVNNLKLNRNLLQQVNE
                     TSQGQFPFAVILSCMDSRTPAELIFDQGLGDVFSIRVAGNILNDDILGSIEFACQVVG
                     VKLIAVVGHTQCGAIKGACDGVKLGNLTNLLNKISPVIQEAKKLDSKHDVHSPEFLNC
                     VTSLNVKHTMNEIKQRSNIVHQLLDEKRIAIVGGLYQLETGEVQFFDE"
     gene            complement(2333..2629)
                     /locus_tag="I9760_001644"
     CDS             complement(2333..2629)
                     /locus_tag="I9760_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043415.1"
                     /translation="MSHPGKKKILFSILYSLRHLIALLVMSVGIYLIKTVTAILYLPS
                     DYSTLPLLSLCGVLWLSNDFFLRFILVVNFIIKPFFLYLGVLFCFYYLHKKCCS"
     gene            complement(2660..3631)
                     /locus_tag="I9760_001645"
     CDS             complement(2660..3631)
                     /locus_tag="I9760_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAT5043416.1"
                     /translation="MSLRILSLSDVKQSITMHQAISALESAFIQLANNTVKLPLRTGV
                     TIEEENGLMLTMPGYLAQEKSLGLKVVSIFPENLTKNKPTITGFIMLLDATTGEAKVL
                     MDAAYLTSLRTGAVSGLATKYFARDNATQVAIIGSGAQAKTQLEAVASVRDIQQVFVW
                     SRNIKNAEKFANEFEDQFDMTICPSVAMAVKDADIICTATNSTEPLIHFGDISPHAHI
                     NAIGSHHPSMKEISHDVLSRAVVIADQLDAVLAESGEIISAVEHNHLKKELIIEIGHW
                     LLQKNTDYKDKLTVFKSVGLAIQDLSVAEAVYQNALKNNLGEIFSIS"
     gene            complement(3686..4132)
                     /locus_tag="I9760_001646"
     CDS             complement(3686..4132)
                     /locus_tag="I9760_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HAT5043417.1"
                     /translation="MKSIANKENEVAIIHKLIEEYANAARDKDIDKIMSYYAPDIRSF
                     DAVSQLQFKGADDYRKHWQTCLSFCPGPSVFEVHELETLVDNNLAVSYYLTYCGGTNE
                     KGEMQGGWMRGTMVHCKMNGKWKIMHEHYSVPFDMKTGNTLFDLKP"
     gene            4211..4381
                     /locus_tag="I9760_001647"
                     /pseudo
     CDS             4211..4381
                     /locus_tag="I9760_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            4575..6056
                     /locus_tag="I9760_001648"
     CDS             4575..6056
                     /locus_tag="I9760_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAT5043418.1"
                     /translation="MPHGVAALCLIQAFSTFSYAVQYSSLALFITKQLGIANLSANSI
                     VGLFLSFSYIFQLIGGVIGSRFISNRLLFVTTILIQNMGLFFLAQANPSLLNIGLSLF
                     MVGSGLNVTSSNSMLTQCFIPQDDRRESAFFLSYAGMNVGYCIGYIACGFFDYSNQYQ
                     YLLYASILTNSIALLIIIKNWAYLGDKNFPLPNLTKRNWYFIKNILAIAFITFLIPFM
                     RVCFEFANFCNGLVIITCLFMFLIIFILGLKQKSSSDRKKMMAYLILAISSVLFWMIY
                     LTGPMGVTLFVKNNVDKHLFSFELATQWIKNINPVVIIFGAPIMSMLINKLKAKGFHV
                     SAPVQFSCSFVFLALSFLCLIFGIICSNSYGYISIYWIIGHHILQGMSELLLGPVGYA
                     LIGRIAPPKLQGILMGTWMLVIGVAASFSQYFSNAMVQSESVDPLITNQDFLTAFKQL
                     GLWSLLCAALLYFVSGKLTRIINGTKDNKSVNESKALPMPYSN"
     gene            complement(6061..6693)
                     /gene="cegC4"
                     /locus_tag="I9760_001649"
     CDS             complement(6061..6693)
                     /gene="cegC4"
                     /locus_tag="I9760_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC4"
                     /protein_id="HAT5043419.1"
                     /translation="MYPNFLGMACLFHKRKNMLTINREKFEEIKNQLKEKQLVCNQIA
                     QWKKTPLWLREMTHQPEIPPNFLNELRQIRDAIVSFEIDKNTLGLAVFYNELYKLLAP
                     SYNVLTPIDTDHMNNHVLLLQRISNYAGKVDEHIKSLSASEVKHLDYAEFWSLLSETC
                     SIQEVDRRKQAKEKFSQSIFLRLCQYNPKIYLDVATRLEEPAEQSNTLDF"
     gene            complement(6859..7395)
                     /gene="cegC4"
                     /locus_tag="I9760_001650"
     CDS             complement(6859..7395)
                     /gene="cegC4"
                     /locus_tag="I9760_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC4"
                     /protein_id="HAT5043420.1"
                     /translation="MSAKQKELERLLELKKKQEEQQVLNQKDMLERIKLENKYMEFLQ
                     MTSQQMEEELKKRGPVKEVEVKGKDIDPIIADYKKLYSKESWYKEPETKDGKTHLTFP
                     SQEAAGTFFKDQAEKNRSFIVIDAATNKVLAYSNGDGKLYNGNGSLYQGGDFKASKED
                     FTSFKMPEREEPKMGMQL"
     gene            complement(7528..8958)
                     /locus_tag="I9760_001651"
     CDS             complement(7528..8958)
                     /locus_tag="I9760_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAT5043421.1"
                     /translation="MDLSLLKKINDFEWQIPQHGKMLVPGVIFASEQLILDMDMKVYE
                     QLSNVATLPGIVSAAYAMPDAHWGYGFPIGGVAAFDPDDGGVISAGGVGFDISCGVRL
                     LSTGLKRDELESVKVSLADALFTHIPAGVGSRGGIHLTIKQLDDMMRGGAQWAVKQGY
                     GEAADLERIEDRGCVEGAIPDFVSEQAKKRQKNEMGTLGSGNHYLEVQEVRQIYCSDT
                     ATALNLHEGDIVVSIHCGSRGLGHQIGTDYLRSMVIESQHYGIKLADRELACAPIHSK
                     LGENYLGAMRAGINCALANREIITHFMRNVFQDILPRTQIDLIYDVSHNTCKEEFHEV
                     DGKKKRLFVHRKGATRALGPGHPQLPKAVSHIGQPVIIGGSMGTGSYVLAGVKGSEHK
                     SFSSACHGAGRAMSRHQAMKKWNGKDIVDKLAQQGILIRSGSYRGVAEEAPGAYKDVH
                     SIVDAAEMSGLAKKVAKLIPIICVKG"
     gene            complement(8992..9384)
                     /locus_tag="I9760_001652"
     CDS             complement(8992..9384)
                     /locus_tag="I9760_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HAT5043422.1"
                     /translation="MHEADIGVRGIGPTLPDAFEMGALALTNVVADAALIKPSLSIKI
                     HCEAPNEEILFTDWLNAVVYNMQIHNMLFREFDVKLNRFVLDAVIRGEKVDQKKHQPV
                     VEVKGATYTELKIYKNNGIWIAQCVVDV"
     gene            complement(9405..9515)
                     /locus_tag="I9760_001653"
                     /pseudo
     CDS             complement(9405..9515)
                     /locus_tag="I9760_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947063.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
     gene            complement(9520..11073)
                     /locus_tag="I9760_001654"
     CDS             complement(9520..11073)
                     /locus_tag="I9760_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase"
                     /protein_id="HAT5043423.1"
                     /translation="MRIFSRFSDRLVIFAFLVIIFVPGIGMFFEKQADEVRSLLNREP
                     HQLPPINIKKIGRTDFKGIENWFVDHTLFMTSLSKFWSHVVYQLGTSIKPGQAILGKE
                     DWLFLGNDYAASIDQYTGRNKPTEEEVLLKLSVLKQMNHLAKQNDIPFLVVIAPDKHE
                     IYPEYLPANVHKSSNKNRLDLLQEGMLARGIDFINLRQKEMEAKNTLGKQYGDLYLKG
                     DSHWNYVGAYVAYQAISDYMRQKGLQSRQLQFNFIPRETTYSDLTNFLQLTHIKSNNP
                     LPDVSNLKIDLFGRDIDGKEIKLDDFQGNPNGVILIAPYENINKAVQNKQTCLLIGDS
                     FSESLSFYFHNDFYNTVRIHSGNTSWNLSDLIQKYHPDLIVYEKVERDLLYPLVNFQT
                     TANQVNLELPKQVLTARGGVDKFKIGPDTISVNGWAYIPDLDAGNGEVFLKLSMGTHT
                     YLYSMNKMQKQSVNLAFKQDGKHLDLSGFNGTISRKDLPSGLYKVSLIVVNDEVMGET
                     ELPGTYTLS"
     gene            complement(11076..12497)
                     /locus_tag="I9760_001655"
     CDS             complement(11076..12497)
                     /locus_tag="I9760_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HAT5043424.1"
                     /translation="MVFSSLIFLFLFLPCLNLTYLVCFKSIKWQNFVLMLFSLSFYYW
                     GEKDHIFIMLACILINYLCGLLIEDSKKPGQRKIYLITSVSASLAMLMYFKYFNFFLD
                     SLNSVFDLNVMGISKIVLPLGISFYTFHALSYVVDVYWKKVPAERNFVTFVCYVSFFP
                     QLVAGPIVRYADIRHSFYQRYITRAGVRNGILRFCFGLAKKVLIANEVAKLADYVFYL
                     PANELTFWVAWLGAIAYALQIYFDFSGYSDMAIGLGLMFGFKYKENFNFPYNALSIQE
                     FWRKWHISLSSWFRDYLYIPLGGSYKSRFRTQINLIIVFGLCGLWHGANYTFIVWGLF
                     HGFFLILERTHFGYFMKKLPKIIRHGYVLSVVTVGWVFFREDNIQSALVFLKTMFIPA
                     EFNLDSIAACINIILIFAFIVGIILALGFNSRVRDSLILSRNKNWIIAYKQSSKVLGI
                     LLLLVSILLLSTNSYNPFLYFRF"
     gene            12713..13351
                     /locus_tag="I9760_001656"
     CDS             12713..13351
                     /locus_tag="I9760_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043425.1"
                     /translation="MQDSFFSFLSSPINLLFLFVAFFLLFVTPWLWNIVNSAHNIHKL
                     LDPLGIKKKENSLPVTGLLFLPLLLIIIFIEWFWPLANITLFPILASPYIKFNIIAVI
                     SVVILLILIQCDKKKSMELWVLAYAAVLYLLYLIIIMATVVVGMNAFPASHYIFAPES
                     SMTINRTIIYGLLIAHLIFIWLTISLKFTWRLLLMILYTLALSFAYFSLIVS"
     gene            13578..14678
                     /gene="wipC"
                     /locus_tag="I9760_001657"
     CDS             13578..14678
                     /gene="wipC"
                     /locus_tag="I9760_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector WipC"
                     /protein_id="HAT5043426.1"
                     /translation="MNRMALFIIFIIHCFFSQSFAEQEKPYNELYVKQANLKQYPKEI
                     NSYPPGVEITIGDLHGNALKLLYFLIRNDVIKIDKEDYKLFVTIYQKNPNELTTKDLS
                     FFQIIVNSAEINTQHKIRFLGDDLCDRGMNDYYTLVIYKKLDQANVPFEVILSNHGNF
                     FLTAYERPEQSFNYNPYGEGENESTVQSMLNMGRLIDRGLIDKQDILEMIQYHYLKHI
                     VLPSYTHNKDKNELTIYTHAPIDLGIISALANDLQVPFKDSNLHELTKSLDAMNSKIK
                     QWILSNTFTRHYKELNEAHNQINTPSPIKQILWNRDYSILDRHAHPNNKPYGINYVHG
                     HDSMPNVFDLDNLFGKGEDFYQGPYAVHITHS"
     gene            complement(14726..>15243)
                     /locus_tag="I9760_001658"
     CDS             complement(14726..>15243)
                     /locus_tag="I9760_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043427.1"
                     /translation="NCSKQKSDQVAINKLSETQAPVDDEIREDILFVDLNKNNSREEH
                     EPIITMSADAYERIFHSQPPLSRMLRRQINNAEYIALSKEEFSKLVLQGNREGLGEIH
                     FVIPVIIMEHGYYETQMQIVNMGKIKEVQPNSDTSRITQEPAKSYRIDFESKPALNLK
                     TTNRFIRWLGL"
BASE COUNT         4737 a         3056 c         2588 g         4862 t
ORIGIN      
        1 actgtatttt tatttaagaa aagcactttt tctttcctgg agttcaagcg gataactaaa
       61 tacgaggcca ttatgaagca tggtttattt gctcatctca aacacgactt accagcatcc
      121 attgttgttt tttttgtggc tttacctttg tgtctgggca tagcattagc ttcacatgtt
      181 cctctttttt caggaattat tgctggtgtt attggtggag tagtcgtagg gtttatcagc
      241 ggctcagcgc ttggagtgag cgggccagca gcaggacttg tagcaatagt catttcctct
      301 gtagagacat taggcagctg ggaagctttt ttgttagctg gggtaattgc tggaatattg
      361 caattaattg caggttttct gcgaggtgga attattgctt actattttcc ctcctccgta
      421 atcaaaggca tgctcacggg aatcggtatt attattgttt taaaacaaat tcctcattta
      481 ttagggcacg acgtcacgcc acttctattg agtatgtcgg atggtgaatt ggatattaat
      541 gacatcagag aagcattgga tgatattaat cctgaggttg ttattatcag cattgtttct
      601 cttttaaccc tgattttttg ggagaagctg gtaaaaaaac acaaactgtt tgaaatgata
      661 caaggccccc ttgtcgtggt catcctggga attgctttga acttgtttta tgaacaccga
      721 ttaaccagtt tttcccttga tccatcggaa ctggttcaac tgccgaaatt taacaaagca
      781 tccgaattat ttaattattt aacactgcca gatttttcgc taattactca tttgaaaata
      841 tacaaaatag caatcctcat tgccctaatc gccagtttgg aaacactgct ctgcgtagaa
      901 gccgttgata aactggatcc ctacaagaga gtaacgtcta cagaccgtga attaaaagcc
      961 cagggagtag gaaatattct ctcttgcttg attggcggac taccgattac acaggttatt
     1021 gtgcgtagta gtgccaatgt cgcttttgga gcaaaatcca agctgtccgc tattttacat
     1081 ggcttattac tttttatttg tgtcatcagc ataccagaaa tactcaatga aattccactt
     1141 gcatcattag ctagtatttt gattattata ggctataagt tagccaagcc ggctttattc
     1201 aaacaaatgt ataaaatggg atgggaacag tttgtaccct ttatgattac aattattggg
     1261 attattttta gcgatttact tttcggaatt acactaggat tttcactagc tatttttatt
     1321 atcttgcgac atcactttct taattcacat gatatgatta aaacccgtgg caaaaacaaa
     1381 acccaatact gccttaaact ggcagaagag gtaacttttt taaacaaagg aagtataatc
     1441 aacgaattaa aaaatatacc agaaggtgcc gaggtagtta ttgatgggtc tcgctctaaa
     1501 attattgatc atgatattaa agaagtcatt caaaatttca tattgaattc aaaaaccaaa
     1561 aatattcaag ttaaattaat aggaatttaa atcatgtgga ctttaaccaa agaacaacaa
     1621 caagcaatta ctccagagaa agctattgag ctcttaaaag aaggtaacaa gcgtttcgtt
     1681 aataatctta agttaaatcg caacttgctc cagcaagtta atgagacgtc acaaggacaa
     1741 tttccatttg ctgttatttt aagttgcatg gattcacgta ctccagcgga gttgattttt
     1801 gaccaaggcc ttggcgatgt tttcagtatt cgagttgccg gtaatatttt aaatgatgat
     1861 attttgggca gcattgaatt tgcatgtcag gtagtaggtg tgaaactgat tgcagttgtc
     1921 ggccatactc aatgtggagc aattaaagga gcttgtgatg gtgtcaaatt gggcaacctg
     1981 acaaatctac tgaataaaat cagccctgtg attcaagaag ccaaaaagct ggattcaaaa
     2041 cacgatgttc atagcccaga atttttgaat tgcgttacca gcctgaatgt aaaacatact
     2101 atgaatgaaa ttaaacaacg cagtaatatc gtacatcaat tgcttgatga aaaaagaatt
     2161 gcaattgttg gtggtttata tcagcttgag acaggagaag ttcaattttt tgatgagtga
     2221 tatcaaggca tcaataattt tcaatgatca gttcttttta gctgtacaac agtattgatg
     2281 tggaatgctt ttatcaaaga attgctgcgg acttgagact tgaagtccgc atctatgaac
     2341 aacatttttt atgcagataa taaaagcaaa ataaaactcc taaatagaga aaaaatggct
     2401 tgataataaa attaacaact aatataaaac gtaagaaaaa atcattggat aaccacaaga
     2461 caccgcacaa agacaaaagt gggagcgttg aataatcaga gggaagatac aatattgctg
     2521 ttactgtctt aattaaataa atacccactg acataaccag tagagcaatc agatgacgta
     2581 aggaatataa aattgaaaaa agtattttct ttttcccagg atgactcatt tatactcact
     2641 ctcataaatg ttgccctgac taactaatag aaaagatctc accaagatta tttttcaatg
     2701 cattttgata aactgcttca gcaacactta aatcctgtat tgccaaacca acggatttaa
     2761 acactgtaag cttatcttta tagtcagtat ttttttgtaa tagccagtga ccgatttcaa
     2821 taatcaattc cttttttaaa tgattgtgct ctactgcact aatgatttcc cctgattcag
     2881 ctaaaacagc gtctaactga tcagcaatca ccactgctcg gcttaacaca tcatggctaa
     2941 tttctttcat tgaagggtga tgcgagccta tggcattaat atgggcatgt ggcgaaatgt
     3001 ctccaaaatg aatcagtggt tctgtgctat tggttgctgt acaaataata tcggcatctt
     3061 tcactgccat ggcgacagaa ggacaaattg tcatatcaaa ttgatcttcg aattcgttag
     3121 caaatttttc ggcattttta atattgcgcg accaaacaaa aacctgttgt atatctcgca
     3181 cagaagcaac tgcctcaagt tgtgttttcg cttgagcacc tgacccaatg atcgcgacct
     3241 gagtcgcatt atcgcgtgca aaatattttg tcgccagtcc tgaaacagcg cccgttctta
     3301 aggaggtgag gtaagcagca tccattaata ctttagcttc acctgttgtt gcgtcaagca
     3361 acataataaa accagtgata gtaggtttat ttttagtcaa attttcagga aaaatggata
     3421 ctacttttaa gcctaagctt ttttcttggg caagataacc cggcatagtc aacattaatc
     3481 cgttttcttc ctcaatggta acacccgttc tcaagggcaa cttcaccgtg ttatttgcca
     3541 attgaataaa tgcgctttcc aaagcactga ttgcctgatg catggtgatg ctctgcttta
     3601 catcgcttaa agacaatatc ctgagactca tatcaataaa ccctatactt tagttaacta
     3661 cgtagtgact gaatagaatc acatcttatg gttttaaatc aaataaagta tttcctgttt
     3721 tcatatcaaa gggaacagaa taatgctcat gcatgatttt ccattttcca ttcattttgc
     3781 agtgtaccat tgtacctcgc atccaacctc cttgcatttc acctttttcg ttcgttccac
     3841 cacaataagt caaataatag gaaaccgcca gattattatc aaccaaagtt tccaactcat
     3901 gtacttcaaa tactgaaggt cccggacaga atgaaagaca ggtttgccaa tgttttctat
     3961 aatcatcagc tcccttaaac tgaagctgtg aaactgcatc aaaagaacga atatctggtg
     4021 catagtaaga catgatttta tcaatgtctt tatcgcgtgc tgcattggca tactcttcaa
     4081 tgagcttatg aattattgcc acctcatttt ccttattcgc aatagatttc acctttatct
     4141 cctttaattg gcttcttcct gaattttaat tgtagcaaat tctgcaaaaa acctaatgaa
     4201 ttgttaacct gcaacagatg ttgctaaagc aacatttttt tgctttgtta aaaaagaacc
     4261 cacaatagaa ttaccctgtt ccctgaggtt ttttgatacc gatgcagcga atggattgcc
     4321 tcatcatata gagaaatctg actctcaaca gaaaaaatat tctgcggaat catttaaata
     4381 aaataaccta tacaattcaa acaggtttga taaacgaagc ttaatcaacg tttctttcct
     4441 aattttacta aagtaaagtt tggaaagaaa ttgaatggca ggttgttaca ttcttgaagg
     4501 caactgatta ttgatggtaa agtctattct cataaaatca gccaaaatgt ttgatgatta
     4561 aaataattcg ggaaatgccg catggagttg ctgcgctctg cttaattcag gcattttcaa
     4621 ctttttcata cgctgttcaa tattcttcgc tcgcactttt tattaccaaa caacttggaa
     4681 ttgccaattt atcagccaat agcatagtgg gcctgttttt aagttttagc tatatttttc
     4741 aactaattgg cggtgtcatc ggaagccgtt ttataagtaa cagactacta tttgttacaa
     4801 ctattcttat tcaaaatatg ggtttattct ttttagcgca agcaaatcct tccttgctta
     4861 acataggctt aagtttgttt atggtaggtt ctggtttgaa tgttacttcc agcaacagca
     4921 tgcttactca atgtttcata ccacaagatg atcgcaggga aagcgcattt tttctcagtt
     4981 atgccggcat gaatgtcggt tattgcatag gttatatagc ctgcggtttt tttgattact
     5041 ccaaccagta tcaatatttg ctctatgcaa gcatcctgac taactccatt gctttattga
     5101 ttattattaa aaactgggcc taccttggcg ataaaaattt tccattaccc aatctcacta
     5161 aacgaaactg gtatttcata aaaaatattc tggcaatagc attcattaca ttccttattc
     5221 cttttatgcg ggtatgtttt gaatttgcaa acttttgtaa tggattggta ataattacct
     5281 gtttattcat gtttcttatt atctttatcc tggggctaaa acaaaaatcc tcctccgaca
     5341 ggaaaaaaat gatggcatat ttaattttgg caatcagttc ggttttattt tggatgattt
     5401 atctaactgg cccaatgggt gtaactcttt ttgtcaaaaa taatgtggat aagcatcttt
     5461 tcagctttga attggctact caatggataa aaaatattaa tcccgtggtc attattttcg
     5521 gagcaccaat catgtccatg ctcattaaca aattaaaagc aaaaggtttt catgtctctg
     5581 ctcctgtgca attttcctgt tccttcgtgt ttttagccct gtccttttta tgccttatct
     5641 ttggaattat ttgttcaaat tcctatgggt atatcagcat atactggatc ataggccatc
     5701 atatcttgca aggaatgtct gagttacttc ttggccctgt tggctatgca ttaattggaa
     5761 gaatagctcc cccgaaacta caagggattt taatgggtac ctggatgctg gttataggtg
     5821 tagcagcctc tttttcccaa tatttctcca atgccatggt gcaatcagaa tcggtagacc
     5881 ccctaattac aaatcaggat tttctaaccg catttaagca attgggactt tggtctttac
     5941 tctgtgctgc cttgttatat tttgtttcag gtaaattaac caggataata aatggtacaa
     6001 aagacaataa atcagttaat gagagcaagg ctctccccat gccttactct aactaatcat
     6061 ttaaaaatct aaagtattgc tttgctcagc gggttcttcc agacgagtag caacatccag
     6121 gtaaattttc ggattgtact ggcacaaacg taagaatata ctttgggaaa atttttcctt
     6181 tgcctgcttg cgcctgtcta cctcttgaat agaacatgtc tcactaagca gagaccaaaa
     6241 ctcagcgtaa tccaaatgct ttacttcact cgctgacaaa ctttttatat gctcgtctac
     6301 cttacccgca tagttgctaa ttctctgaag caaaagcaca tgattattca tatgatcggt
     6361 atcaattggt gttaatacat tgtacgatgg agctaaaagc ttatacaact cattataaaa
     6421 cactgccaaa cctaaggtat ttttatcaat ctcaaaggat acaatagcat cccgaatctg
     6481 tcttaattcg tttaaaaaat tggggggaat ctctggctga tgagtcattt ctctcaacca
     6541 cagcggagtt tttttccatt gcgcaatttg attgcaaact aattgtttct cttttagctg
     6601 gtttttaatt tcctcaaatt tttctctatt aatagttagc atgttttttc tcttatgaaa
     6661 aaggcaagcc atacccaaga aatttgggta cagtttccag aattggtaat atttgataaa
     6721 gcttaaactt tttcagatta catgatatga tatttgacaa ttaacgtcaa aaaaagtcag
     6781 tctggttttt atttaaaaaa gagaaaaaac aatcataaaa tgattatcgg cactaaatcc
     6841 gatttaaatc aaagcagatt aaagttgcat acccatttta ggctcttctc tttcaggcat
     6901 tttaaagcta gtgaaatcct cttttgacgc cttgaagtca ccgccttgat aaaggctacc
     6961 attcccattg tatagttttc catcgccatt agaataggca agcactttat tggttgcagc
     7021 gtcaataaca ataaaagatc tgtttttttc agcctgatct ttaaagaagg tacctgctgc
     7081 ttcctgagac gggaaagtca aatgagtttt tccgtctttc gtttcgggtt ctttatacca
     7141 actttcctta ctataaagct ttttgtagtc tgcaataatg ggatcaatat ctttaccctt
     7201 cacttcaact tcttttacag gacctctttt ctttaattct tcttccattt gctggctggt
     7261 catctgcaaa aattccatat atttattctc gagctttatt cgctccagca tatccttctg
     7321 atttaaaact tgctgttctt cctgtttttt ctttaattcg agtaatctct caagctcttt
     7381 ttgttttgca ctcattatag atatctcctc gcacttgttg agctaatttt agtctaaaaa
     7441 tttatcgaaa attattttga gttcattttt tttacaaatt ccttacaatt ataagctgct
     7501 cctttcacct caataaacaa tggattgtta acctttgaca caaatgatgg gaattaattt
     7561 agcaactttt tttgccaaac ccgacatttc agcggcatcg acaatagaat gaacatcttt
     7621 ataagccccc ggagcttcct cagcaacccc acggtatgat ccgctacgaa ttaaaattcc
     7681 ttgttgagcc aatttatcta ctatgtcttt cccgttccat ttcttcattg cctgatgacg
     7741 actcatagcg cgacctgctc catgacatgc cgagctaaaa gatttatgct cagaaccttt
     7801 gacgcctgct aatacatagg aacctgttcc catgctccct ccaataatca caggctggcc
     7861 tatatgagaa acagctttag gtagttgtgg atggcctggg cccaaggcac gagttgctcc
     7921 ttttcgatga acaaataagc gctttttctt gccatctacc tcatgaaatt cttctttaca
     7981 agtattgtga gaaacgtcat agattagatc aatctgagtg cggggtaaaa tatcctgaaa
     8041 cacattgcgc ataaaatgag tgatgatttc tctgttggct aaagcacaat taattcctgc
     8101 tctcatagcg cccaggtaat tttcccctag ctttgaatga ataggagcac aagccaattc
     8161 cctatccgcc agcttgatac cataatgttg tgattcaata accatggaac ggaggtaatc
     8221 tgtaccaatt tgatgcccca atcctctgga accacaatgg atactcacta caatatctcc
     8281 ttcatgcaaa tttaaagctg ttgctgtatc ggaacagtag atttgcctga cttcctgcac
     8341 ttccagataa tgattacctg aacccaaggt acccatttca tttttctggc gctttttagc
     8401 ttgctcagag acaaaatcag ggatagctcc ttccacacaa ccgcgatcct cgatacgctc
     8461 taaatcagca gcttcaccat agccttgttt tactgcccac tgtgcacccc ctcgcatcat
     8521 atcatcgagc tgtttgatag ttaaatgaat gccgcctcta ctacctactc cagctggaat
     8581 atgagtaaat aaagcgtctg caaggctaac tttgacagac tccaattcat ctcgcttcaa
     8641 tccagtagat aagagcctca ctccgcatga aatatcaaaa cccacgccac ctgctgaaat
     8701 aacccctcca tcatccggat caaaagcagc gacaccgcca atcggaaaac catatcccca
     8761 atgcgcatca ggcatagcat aagcagcaga aacaattccc ggcaaagtag caacattaga
     8821 taattgttcg taaactttca tatccatatc aagaatcagc tgttctgaag cgaaaataac
     8881 tcctggcacc aacatctttc catgttgcgg tatttgccat tcaaaatcat ttattttttt
     8941 caaaagactt aaatccatca ctgttcacca cattaattcc tgttaaggag tttatacatc
     9001 aacaacacat tgagctatcc aaatgccatt atttttgtat atttttaatt cagtataggt
     9061 ggcccctttg acctcgacaa ctggttgatg ctttttttga tcaacctttt cacccctaat
     9121 gacagcatcc aaaacgaagc ggttgagttt tacatcaaac tcacgaaaca gcatattatg
     9181 aatttgcata ttatagacca cggcatttag ccaatcggta aataaaattt cttcattggg
     9241 tgcttcacaa tgtattttta ttgataaaga aggcttgatt agggctgcat ctgcaactac
     9301 attcgttaat gccaaagctc ccatttcaaa ggcatctggg agagtaggtc cgatgccacg
     9361 aactccaata tctgcctcgt gaatgaagtg ttcccagtat ttatttaatg gtaacgggtt
     9421 ttgtcttttg ctaataatcg caactccttt ttagcttcca ttaaaatttc tttttgcccg
     9481 gtagccaatt tgcttccagc tcgattataa aaatcccatc taactcaaag tataagtgcc
     9541 tggaagctcc gtttcgccca tcacctcgtc gtttacgaca atgagtgata ctttatatag
     9601 gccagaaggc aaatccttac gcgagatagt tccattaaat cctgacaaat caagatgctt
     9661 accatcctgt ttgaaagcca gattaacact ttgtttttgc attttattca tactgtataa
     9721 ataagtatga gttcccatcg acaactttaa aaacacttcc ccatttccag catctaaatc
     9781 gggaatataa gcccagccat tgactgagat tgtatctggg ccaatcttga atttatcgac
     9841 tcctcccctg gcggttaaaa cctgtttagg gagttcaaga ttcacttgat ttgctgtagt
     9901 ttgaaaattg acgagtggat agagcaaatc acgctcgact ttttcataca caatcaaatc
     9961 aggatggtat ttttgaatta aatcagacaa attccaggag gtattcccgg agtgaatacg
    10021 tactgtatta taaaaatcat tatgaaaata aaaactgaga gactccgaaa agctgtctcc
    10081 tattaataaa caggtttgtt tgttttgtac agctttatta atattctcgt aaggcgcaat
    10141 gaggattaca ccatttggat tgccctgaaa atcatccaat ttaatctctt ttccatcaat
    10201 gtctctgcca aacaaatcga tttttaaatt tgaaacatcg ggaagaggat tattggattt
    10261 gatatgcgtc aactgcagaa aattggtaag atcagagtag gtggtttctc tgggtatgaa
    10321 attaaattgt aattgtctgc tctgtaaccc tttttgccgc atgtaatctg atattgcctg
    10381 gtaagcaaca taagcaccaa cataattcca gtgactatcc ccctttaaat aaagatctcc
    10441 atactgtttg ccaagagtat ttttggcttc catttccttt tgtctcaaat tgataaaatc
    10501 aatacctcga gcaagcattc cctcttgtaa aaggtcaagc ctgttcttat tggagctttt
    10561 atgaacgtta gccggtaaat attccggata aatctcatgt ttatctgggg caatgaccac
    10621 cagaaaagga atgtcatttt gtttagccag atgattcatc tgttttaaaa cagataattt
    10681 caagagaact tcttcctcgg taggcttatt tctccctgtg tactgatcga tggatgccgc
    10741 gtaatcattg cccaaaaaca gccaatcctc tttccccaaa atggcttgcc caggtttgat
    10801 gctggttccc aactgatata cgacatgact ccagaattta ctcaacgaag tcatgaacaa
    10861 ggtatgatca acaaaccaat tttctatgcc cttgaaatca gtacgaccta ttttttttat
    10921 attaatggga gggagttgat gaggttctct gtttaacaga gatctcactt catccgcttg
    10981 tttttcaaaa aacataccaa tccctggtac aaaaatgatt accagaaaag caaagatgac
    11041 cagcctgtca gaaaacctgg aaaaaatacg catcatcaaa acctaaaata gaggaaaggg
    11101 ttataagaat tagtgcttaa taataatatt gaaaccagta aaagcagtat tcccaaaacc
    11161 ttgctggatt gcttgtatgc gataatccag tttttatttc tacttaagat gagactatcc
    11221 cttactctgc tgttaaaccc taacgcaagt ataatgccca caataaaagc aaaaataaga
    11281 ataatattaa tacatgctgc tatggaatca agattgaact cagcaggaat aaacatcgtt
    11341 ttaaggaata ccagtgccga ctgaatatta tcttccctga aaaataccca tccgactgtc
    11401 acaacagaga gaacatagcc atggcgaata attttaggta attttttcat aaaataacca
    11461 aagtgggttc tttccagaat aaggaaaaac ccatgaaata acccccaaac aataaaggta
    11521 taattagcgc catgccataa accgcacaaa ccgaatacaa tgatcaagtt aatctgcgtt
    11581 cgaaatcttg acttataact ccctccgagc ggtatatata aataatctcg aaaccagctt
    11641 gataaggaga tatgccactt ccgccagaac tcctgtatcg ataaggcatt atagggaaaa
    11701 ttaaaatttt ccttgtattt aaacccaaac atcaaaccaa ggcctattgc catatcagaa
    11761 tagccggaaa aatcaaagta gatttgcaga gcataggcta tagctccgag ccaggccacc
    11821 caaaaagtca actcattggc aggcaaataa aatacataat cagcaagctt tgccacttca
    11881 ttggcgataa gaaccttttt tgccaagccg aaacaaaaac gcagaattcc attacgtacc
    11941 cctgcacgag ttatatatct ctgatagaaa gaatgtctta tatctgcata gcgaacgatt
    12001 ggtcctgcca ccaattgcgg gaaaaaggat acataacaaa cgaaagtaac aaaattacgt
    12061 tctgctggta cttttttcca atacacatca accacatagc ttaaagcatg aaaagtataa
    12121 aaacttattc caagaggcag cacaatttta ctgattccca tgacatttaa gtcaaagaca
    12181 ctgttcagtg aatcaagaaa aaaattaaag tacttaaaat acatgagcat agccagtgat
    12241 gcgctcacag aggtgatcaa atagattttt cgttgtcctg gttttttgct gtcctcaatc
    12301 aatagaccac atagataatt tatcaaaatg cacgccagca taataaagat atgatctttc
    12361 tcaccccaat aataaaagga caagctaaag agcattaaaa caaaattttg ccacttgata
    12421 cttttaaaac agactagata agtcaggtta aggcacggaa gaaataaaaa aagaaatatt
    12481 aaagatgaaa aaaccattct atctccgtga aggcatttgc ggaattaatt aaacttgtat
    12541 taactgttca agacgttaga ttcatcgcgt gaagcgcatt atagggaagt gttattcgtt
    12601 gtgcaaatag aatacgataa aaagaaccat ctgcaaaatc tgaattgaag ctgccattat
    12661 aacattcatt agctatactt aacctaaggc agcattcgtt gatgaattaa taatgcaaga
    12721 ttcctttttc tcatttttat caagccctat caatttactc tttctttttg ttgcattttt
    12781 tttgcttttt gtaacaccat ggctctggaa catagtgaac tcggctcaca acattcacaa
    12841 gcttcttgac ccgcttggga ttaagaaaaa agaaaattca ctgccagtga caggactgtt
    12901 atttctccca ctgctcctga taataatttt cattgaatgg ttttggcctt tggcaaatat
    12961 cacgttgttt ccgatactag cctcacccta tattaaattc aatatcattg cagtcatttc
    13021 agtagttatt cttttgattt taatccaatg tgataaaaaa aaatcaatgg agctttgggt
    13081 gcttgcctat gcagctgtac tttatttatt gtatttgatt atcattatgg cgaccgtagt
    13141 ggtagggatg aatgcttttc ctgcaagcca ttatattttt gcccctgaaa gcagcatgac
    13201 aatcaatagg acaataattt atggtttatt aatagcccat ttaattttta tctggctaac
    13261 catatcgctt aaattcacat ggcgtttatt gttaatgata ctctatactc tggccctgtc
    13321 ttttgcttat ttctctctta tagtctctta aataagctgt ttctttcatt ttattgtagt
    13381 gttgcaaacg tttgtgtggt gcagaaactc cgcccctcaa aacgttttca ctttaccctg
    13441 gcaggactta cgaaaaaccc caaggtcgag gcaagtggtc taccattgct ttcatgaaca
    13501 aaccaaggta tacctagctt accagattac ggtcatcgac acagaacaat aaaaaatttt
    13561 tacaggatgt agcattcatg aaccgcatgg cacttttcat catttttatt attcattgtt
    13621 ttttttctca atcatttgcc gaacaggaaa aaccatacaa cgaattgtac gtcaaacaag
    13681 caaacctgaa acagtatccc aaggaaatca atagttatcc gcctggcgta gaaatcacta
    13741 ttggagattt gcatggaaat gcattgaaat tgctctattt tttaattcgc aatgatgtga
    13801 ttaaaatcga caaagaggac tataagctat ttgtaactat ctaccagaaa aatcctaacg
    13861 aactgacaac aaaagatttg tcttttttcc aaattatagt caattccgct gaaatcaata
    13921 cccaacacaa gatacgtttt ctgggagatg atctatgtga tcgtggcatg aatgactatt
    13981 acactttggt tatttataaa aagctcgacc aggccaatgt cccttttgag gtgattctat
    14041 ccaatcatgg caactttttt ttaacagcct atgaaagacc tgagcaatct tttaactata
    14101 atccttacgg tgaaggtgaa aacgaatcca cagtgcaatc catgctaaat atgggaagac
    14161 tgattgatcg gggattaatt gataaacagg acattttgga aatgattcaa tatcattact
    14221 taaagcatat agtacttccc agttatacgc ataataaaga taaaaatgaa cttactattt
    14281 atactcatgc gccaatcgat ttaggaataa tctcagcatt agccaatgat ctgcaagttc
    14341 cttttaaaga ctctaacttg catgaattaa ccaaaagtct tgacgcaatg aactccaaaa
    14401 taaaacaatg gatactctct aacacgttca ctagacatta caaggagctt aacgaagcac
    14461 acaatcagat caacacgcca agcccaatta agcaaatatt gtggaatcgt gattattcta
    14521 tcctcgatcg ccacgctcac cccaataaca aaccctatgg cattaattat gttcatggcc
    14581 atgactctat gcccaatgtg tttgatctgg ataacttatt tggaaaaggt gaggattttt
    14641 accaagggcc ttacgctgtg catattacac acagctagct gtgaataaaa tcaaccctgt
    14701 cattaaacag ggtaacaaat tgaaattata gccctagcca ccgaataaag cgatttgttg
    14761 tttttaaatt gagggctggt tttgattcaa aatctattct atagctttta gcaggttcct
    14821 gtgtaatacg agacgtatca gaattgggtt gaacttcctt aattttaccc atattgacga
    14881 tttgcatttg agtttcataa tacccatgtt ccataataat gacaggtatt acaaaatgaa
    14941 tttctcccaa accctcccta ttcccctgaa gaaccagctt gctaaattct tccttactca
    15001 aagcaatgta ttctgcattg ttaatttggc gtcttaacat gcgggacaaa ggaggttgag
    15061 aatgaaaaat acgctcataa gcatctgctg acatagtaat gataggttca tgctcttcac
    15121 ggctgttatt tttattgagg tcaacaaaca aaatatcttc ccgtatttca tcgtcaacag
    15181 gagcttgggt ctcagaaagc ttgttaatag cgacttgatc agatttctgt ttactgcaat
    15241 tta
//
LOCUS       DACTJE010000070        15167 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.070, whole genome shotgun sequence.
ACCESSION   DACTJE010000070 DACTJE010000000
VERSION     DACTJE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15167
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.070"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..237)
                     /locus_tag="I9760_001659"
     CDS             complement(<1..237)
                     /locus_tag="I9760_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calmodulin"
                     /protein_id="HAT5043428.1"
                     /translation="MVYYINLKEQPLHQDKEILTQLIKQYLLSGSSQSALFANHPYTL
                     NDEDGNEIEFLLSHDIYKNQREDGITEFLILNGSQ"
     gene            595..1509
                     /locus_tag="I9760_001660"
     CDS             595..1509
                     /locus_tag="I9760_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT5043429.1"
                     /translation="MDLRKINLNLLLHLDTLLNELSVSKAAEKSFMSQTAMSHILKQL
                     RDIFDDPLFIRKPHGLQPTQKVLDLAPKIKKFLNYSNDIFEEDTFNPGNDNLFLKTAS
                     VGTGEYFILPRLCAHLAKNAPKFVLKISSLSEYLSLEYLLMSELDFAIGPGFVETGNN
                     IIRELLFEEEAVCIMHKSHPLANQELTQDMYLQAEHVDIQFSYMGNENILYKALQGYH
                     QRNIKVIVPNIISALEVVYHTNFITTVPRTLAVTFKDRYQIELHPFPFPKKTFLVNFY
                     YHKRLSNHKPLQWVLDVIKKYCCVNHEA"
     gene            complement(1521..2111)
                     /locus_tag="I9760_001661"
     CDS             complement(1521..2111)
                     /locus_tag="I9760_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAT5043430.1"
                     /translation="MVAMNNPISLLTTWISEELEKGAPNPQHAILSTAGKNSIPHGRV
                     IAIREINEQGLLFFTQRGTRKVEEMTHNPMVSLTFWFELCEREVMIDGSARPLSESEN
                     QAYWDTYPPFAQIRFHSYAPTSSQVIPSKQLIENKRKILTSQFHDKPLPMSEYYYGFR
                     VIPTRMVFYTYRTDELSDVVEYCFIKNNWEKNLLSP"
     gene            complement(2242..2622)
                     /locus_tag="I9760_001662"
     CDS             complement(2242..2622)
                     /locus_tag="I9760_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5043431.1"
                     /translation="MVQIIEMDEKKLIQKKAPIKTASMVEAIYGCKWSLTIYSLLRNG
                     INRPGEMVRSVEGLSTKVLNQCLRKNIEFGILGRNTFHELPPRVEYFVTPFGEKFLKI
                     LDQIEELNEQIRDYQQPKFHNDDS"
     gene            2717..3481
                     /locus_tag="I9760_001663"
     CDS             2717..3481
                     /locus_tag="I9760_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAT5043432.1"
                     /translation="MFNQNLAIIIGGSSGMGLETAKKLSSNNMELLITGKDKQKLENA
                     AAEIQKLGAKVDILVLDLYDKEQVFQFMTSMNDETRRVKYLVNAAGYFKPITFLEHDE
                     EDYDRQVNLNKAFFFITQAVVKNMKKNGGGSIVNIGSMWANQAVKATPSSAYSMQKAG
                     LHSLTQHLAMELAEHNIRVNAVAPAVVATPIYKSFIDEERIEESLQDFNKFHPIGRIG
                     KATDIADAIEFLLSEKASWITGEIMNVDGGVMAGRN"
     gene            3504..3764
                     /locus_tag="I9760_001664"
     CDS             3504..3764
                     /locus_tag="I9760_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043433.1"
                     /translation="MPASINSKRLIFNALFQIYLMWVLDKDYIRSSQYPESVWAEEMR
                     KHRLEALARNIKFHTRLRDGINASSARTIQNNGCLIHNSRYP"
     gene            complement(3859..4800)
                     /locus_tag="I9760_001665"
     CDS             complement(3859..4800)
                     /locus_tag="I9760_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAT5043434.1"
                     /translation="MILNITAEQFPDLTLNAIKSCQNIYQSIDFNFGEDADIAINKTS
                     LEKFINQFKSIHSTHDKPIEGIITLGKMKNVSSDTIKLLLTTEDFVQMLDQKSFLKLI
                     VTSNEIANFVLDNPKLRAKLDGIEPLVDAQKFENSCTARAIMKILLERGFIEPSSYTP
                     GKELEIYKDIWLEPGKVASPEKIASYFCKYNLNVIGVEIRELSKSVRNKYSKDMVITS
                     LYSLFKKEVPIRKKMTLTTLSEADFPEGITSLIIIKAGVLHTLLGKKRHGQFEVTDPW
                     FGDKKIYSGFMDFLEKERKNLGVFFEISQGSQEIFRP"
     gene            5033..5551
                     /locus_tag="I9760_001666"
     CDS             5033..5551
                     /locus_tag="I9760_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-box protein"
                     /protein_id="HAT5043435.1"
                     /translation="MKKNFFSDLPEETIVNTLSFLKANTLARIAQTCQFFNRLANDKH
                     LELHQLRQQHIKRELWGNLMVAARSNNLEEVKKILKKGIDPTQTNSYHLNRTPLLAAI
                     EGKAYQTANYLWRKYTFDPNFKDNYGDSPISLLKKQLANPAFKDKEKKQIRALIRGMQ
                     EEKIAQSKCLVC"
     gene            complement(5582..6001)
                     /locus_tag="I9760_001667"
     CDS             complement(5582..6001)
                     /locus_tag="I9760_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT5043436.1"
                     /translation="MRAKNDECTHVLLVDDDEVDLMSVQRALTKLSIPVCFHIARDGV
                     EALEQLSGNIKATKPKIIILDIMMPKMNGIEFLRELRSHIHYNPIKVVIFTTSSNIRD
                     KQATTGLDIAGYFVKDSQFNEFVYCCKHLLEKKSHLS"
     gene            complement(5991..8441)
                     /locus_tag="I9760_001668"
     CDS             complement(5991..8441)
                     /locus_tag="I9760_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT5043437.1"
                     /translation="MRRENTKSNIDFKSIFDSLPDLYLLLDRDFNIVGVSDAYLQATM
                     VSRKQILGKNVFDAFPDNPNDPKATGAKNLYASLLRVLKNKSADTMAIQKYDIQRPAD
                     RGGGFEERYWSPINTPVLGADQEVEYIIHRVEDVTEFVHLKKTGSAQLKLMEELRTKA
                     GEMEIEIYQRAQEIQEANKQLEEANKNLARLDQIKTQFFANVSHELRTPLMLILDPLE
                     MLLAEKKLPSFVQNTLRRIQNNAQILLKHVNDLLNISKLDAGKMILHYANIDLVKLIQ
                     QTIALFEAQIPERKLVFSMDLPDELQAQIDPDKIQHVLINILSNAIKFTPQNAKVRIR
                     LVQINPETCRLQIEDNGPGIPSDLCETIFERFFQIEESTTRTVGGTGLGLSIAKDFVE
                     LHGGSILAGNARNGGAIFTIELPLKAPQNAKVYSSYSQLLLGEKEISASLAEFRNKVK
                     PIKEGAEEYFEQDYYDRPLILVVEDNQEMNHFICKILNTFYRTESAFDGREGLQKSLA
                     LHPDLIVSDIMMPHMSGVEMVHAIREHPLLLKTPIIILTAKADDELTARMLEEGAQDY
                     VIKPFSAKEFKARVANLILVKKAEDELDQFVYHASHDLKAPLPAMEHLISWIEEDLGE
                     MLSEQSKKYLLLLRQRASRMSKLLDGLLKYAQSGYVFDKVRTIHTDRLITDIIKKLNP
                     PQTVTFQLEDTLPVLNTCELPLREVFTALLDNSIRHHHRKDGHIIIGTKEKDRFYEFF
                     VADDGPGIEKKYQDRIFQLFQTLQPRDKLETSGVGLSIAKKIVEFQGGKISVESEKNK
                     GSVFRFLWPKDKEIGDAC"
     gene            8855..10303
                     /gene="mavC"
                     /locus_tag="I9760_001669"
     CDS             8855..10303
                     /gene="mavC"
                     /locus_tag="I9760_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavC"
                     /protein_id="HAT5043438.1"
                     /translation="MTTSKLEKTGLHVHEKIKHMVKNYGTMITGIPAEILGQNEAEIS
                     VGYVKKMGNMKENIAEVVRKSEMTQPTNSCGKASNEVCDLLLGTEGASEFEKSSYQVL
                     SGDGSNLKGSLPNKNLLVRVEMDRFNAPQKYQKIKREEFNPETAEKNKIYLLEDQLVY
                     LDIFGKVIDLGQTSDTCHRLFNAITTPFYQNYILYDEYIDPEESAEEAAMFEMGEIVK
                     AKMKNIDCWTATHSFTIFVPESDSEDTRTLYPYQAYWTSHTLQQWFSGDKDEKLSRLG
                     IDGYIEKLALLGTTTDSKIRSSIYGELFSPPGKEHVFCTGMNEKFSPLRVKFKVTEVN
                     PEIALQNLEEVQEFIDTNYPGENAKDQCELYKIKAQEAMTKQLEMRLLIEPTHRKESV
                     TPYKEKNTQSFFSKNLDTTSRIDKMISSVITMENLKILAKEADTLSGKDREKLVEYFK
                     TLPSEQLAEIKAESETAQKAWDEQYGLVLRDK"
     gene            10379..11659
                     /locus_tag="I9760_001670"
     CDS             10379..11659
                     /locus_tag="I9760_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2148 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043439.1"
                     /translation="MIVRGINMTKIKLESPGFMVHKKLKSMSQSYGVMMTGVPAEVLG
                     QMQAERSIPSINKTGNLKQQIAKEVSKVCHMMTEPTQSCGQASNDVCELLLGKIEAEK
                     FHFTKYEALSADGDNLKNVLENTAPSSTNLLIRFEIDREDPPIVLVKTKNENFNPETA
                     VKNKIYLLENKLYFIDKMGNLFNLGPGKKKCTQLFNAIGDSAEYSLCDPFVLEEPEKP
                     EDFAISEIVDIFNEQKERFDFWIGSHSFTIYIPQTLGESPRQFYPYQAYFGSHTLQDW
                     FVSDKDEYLSRIGIDKYIEKLAVLGKTTNTKERSDIYAEFFSKRGREAFFCAHLNEKR
                     QPLRVKFKITEINPELALKNLQETQEFIDTHPGENPSDKVENYRNRAKLAMTEHLESL
                     LDIKPESSPSLFWKNVDKKEQEMTDSSRYKKGPS"
     gene            11747..12106
                     /locus_tag="I9760_001671"
     CDS             11747..12106
                     /locus_tag="I9760_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2149 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043440.1"
                     /translation="MMSREKAAGTFFKDYQKKNVMRLLQDSLEKIINEWLKTDDESHT
                     KLKSLQELSEMDINATSFAEHSPLPDFVTRLWLDPHKALDAMDKNISKNEIRKLIKET
                     AREIELVFTHQKAPDYS"
     gene            12340..12921
                     /locus_tag="I9760_001672"
     CDS             12340..12921
                     /locus_tag="I9760_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043441.1"
                     /translation="MSCYILDLIETLKNDFVKKNTPTRFDSVKSYGELLEKSKINPAI
                     AEYITGTSSGVMMVDSYEKLLELSKINYAVADNMTQTQLGLDLIDSYEKFLEITKINA
                     KVAFNFQILNYQRYVKLVDSYQQSSQAKSLSDLNTSANSQLFFKKRDSTGQDRASITT
                     GQARVAHDTVGLGLKWNEAIQNTISENMMNQRL"
     gene            12980..13846
                     /locus_tag="I9760_001673"
     CDS             12980..13846
                     /locus_tag="I9760_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HAT5043442.1"
                     /translation="MKNRRDEQTMLTLIIKIANDDERIKIVIMNGSRASPSAKKDIFQ
                     DYDIVYLVTEIESFVHDKNWINQFGELLIMQTPDEMDGQWPKFKGKYTYLMQFKDWNR
                     IDLTLLHIDQLETMPRDSQSLLLLDKDNLIAPFGEPTDEDYLPSPPTEKDFVNCCNEF
                     LWVSTYVAKGLWRKQLIYAKHVSEQIVKEELIKMLMWYAGIQTNFSQPMGTYGKYIEN
                     HLELELRQEFLKTYVDADYKNMWASLFKMCEIFNDLAIKISSYFGYSFNQKEFDNVVD
                     YLQDVKNNKIPT"
     gene            13979..>15167
                     /locus_tag="I9760_001674"
     CDS             13979..>15167
                     /locus_tag="I9760_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5043443.1"
                     /translation="MPKTPNNCSLSSGNKPPTFLRILFSYMAEYRWNTAFFILFGLFW
                     AFTLPYMSYLLGQIIDEIKFQNPKQFDTFSLILTSLILYVAIHVLRSLGYYVHGLFSL
                     TSIPEKKAKLIKQLFNHLGNQSIGYFEDKHSGYLTNKITNASSSIEPLIFNLFTIIYP
                     QSLAIILTGILLSLVVPYFGVVLWLWGAILIIYSYHSAKIGNDKAMLFANSNSKVNGK
                     VVDVVNNIHTVIYNAEMSFELGNLDTDIQDMVQNDRNLQRHMTKVMLVQHIAMNILVA
                     FFLIGSVLGYNYGYVSVGDIVFVMTSVTAIAGLTSSLGKCFLDFVTNAGRLNEGLSLL
                     GDQHTITDNANAQAHQINNGEIQFHDISFAYPNQPPIFLNFNLTIPAKQKIGIVGSSG
                     AGKT"
BASE COUNT         5073 a         2756 c         2657 g         4681 t
ORIGIN      
        1 ctgtgagcca tttaaaatta aaaactccgt aataccatcc tcacgttgat ttttgtaaat
       61 atcatgactt aagaggaatt ctatttcatt tccatcttca tcgtttagcg tataaggatg
      121 gttggcaaat aatgcggact gactggaacc agacaataaa tattgcttaa ttaactgcgt
      181 taaaatttct ttatcttgat gtaaaggttg ttccttcaaa tttatgtaat aaaccataat
      241 gctctcctac aagccaatta tctacatgta tttgagcata ttgataaata ttaatacaat
      301 taattgtttt gatatgtttt attaaattta gttatatcta ttatttttca atgaattact
      361 gatgatttga cgtcttttta tgagcagtta tttattttga taaatatcaa aaaatattat
      421 taacaactcc tattcatatt cataatactg tttgttagaa acaaactgtt tccttaacta
      481 taattctatt aataaagaaa aagatatggt cggtaaattc caaattgtag tttttgggct
      541 tattcagtca ccctggatag gaatataaaa aagttaattt ggatttaatg aaaaatggat
      601 ctcagaaaaa ttaatctcaa cttgttattg catcttgata cattgttaaa tgagctctca
      661 gtatccaaag cggctgaaaa atctttcatg agccagactg caatgagcca tatattaaaa
      721 caattacgtg atatattcga tgaccctttg tttattagga aaccgcacgg gcttcaacca
      781 acacaaaagg tacttgatct ggcccccaag attaaaaaat ttttaaatta ctccaatgat
      841 atttttgaag aagatacttt caatccagga aatgacaatt tatttttaaa gactgcatca
      901 gtaggtacag gggaatattt tattttaccc aggttatgtg ctcatttggc taaaaatgct
      961 ccgaaatttg tattaaagat ttcttcactg tcagagtact tgagtctcga gtatttatta
     1021 atgtccgaat tagactttgc aataggacca ggctttgttg aaacggggaa taacatcatc
     1081 agagaattat tgtttgaaga ggaggctgtg tgcatcatgc ataaatctca tcctctcgca
     1141 aatcaggaat taactcaaga tatgtacttg caagctgaac atgttgacat acaatttagt
     1201 tacatgggaa atgaaaatat actgtataaa gcattacaag gatatcatca aagaaatatc
     1261 aaagtgatag tgcccaatat cattagcgca ttggaagtgg tttaccatac gaatttcatt
     1321 acaactgtac cgagaactct agctgttact ttcaaagaca gatatcaaat tgaactacac
     1381 ccttttcctt tccctaaaaa gacattttta gttaattttt actatcataa acgattatca
     1441 aatcataaac cgctgcaatg ggtattggat gtaataaaaa aatattgttg cgtcaatcat
     1501 gaggcttaaa caaattgata ttaaggagaa agcagattct tctcccaatt gttttttata
     1561 aaacaatatt ctacgacatc agacaattca tccgttctat aagtataaaa aaccatgcgt
     1621 gtgggaatga ctctgaaacc ataataatat tcactcatag gcaatggctt atcgtgaaac
     1681 tgagaagtca atatttttct tttattttca atgagttgtt tgctgggtat aacttgtgag
     1741 gaagtaggcg cataactatg aaatctgatt tgcgcgaaag gcggataggt atcccagtat
     1801 gcttgatttt cgctttccga taatggcctg gctgaaccgt ctatcatgac ctctctttca
     1861 cataactcaa accaaaaagt gagagatacc atagggttat gggtcatttc ttcaactttt
     1921 ctggttcctc gttgggtaaa aaataataaa ccttgctcgt tgatttcgcg aatggcgata
     1981 acacgcccat ggggaataga gtttttgcct gctgtagata atatggcatg ctgtggatta
     2041 ggtgctcctt tttccaattc ctcagaaatc catgttgtta aaaggctaat gggattgttc
     2101 atggccacca tttgaatacc tattgcaaat attttttgat gttcataatg atactgatta
     2161 acaacagctc attgcgacgc tgagtattgt gagccataat tcacccgcag tctattggta
     2221 atacgtcttt tacaaaaaat cttaactatc atcattgtgg aacttgggtt gttgataatc
     2281 cctgatttgc tcgtttaact cttctatttg atccagaatt tttaaaaact tttctccaaa
     2341 aggagtcaca aaatactcaa ctctgggagg tagctcatga aaggtatttc tgccgagaat
     2401 cccaaattca atattcttcc gaaggcattg gtttaaaact tttgttgata gtccttcaac
     2461 gcttcgtacc atttcccctg gtcgattgat tccatttcgt aaaagagaat agatagtcaa
     2521 ggaccactta caaccataga tagcctctac cattgatgcg gtcttaatgg gagctttttt
     2581 ttgtattaat tttttttcat ccatttcaat aatttgtacc ataaagtact tagtgaactg
     2641 aaaagtgctt acttttttta atattgtagt tctttcatac tggattggac agtattttaa
     2701 tgaatcagga gaatctatgt tcaatcaaaa tttagctatt attattggcg gctctagtgg
     2761 tatgggcctt gaaactgcaa aaaaactttc aagtaacaat atggagctgt taattaccgg
     2821 aaaagataaa caaaaacttg aaaatgctgc ggcagaaata caaaaactgg gagcaaaagt
     2881 agacatttta gttcttgatc tttatgacaa agagcaggta ttccaattta tgactagtat
     2941 gaatgatgaa actcgaagag taaaatatct tgtaaatgcc gcaggttatt tcaaacccat
     3001 cactttttta gaacatgatg aagaagatta cgatcgtcag gttaatctta ataaggcgtt
     3061 cttttttatc acccaggccg tagtaaaaaa catgaaaaaa aatggagggg gtagtatcgt
     3121 caatatcggt tcaatgtggg cgaatcaagc ggttaaagca accccctcct ctgcttattc
     3181 catgcaaaaa gccggacttc attcactaac ccaacattta gctatggagc ttgctgagca
     3241 caatataaga gtaaacgccg tggcaccggc agtggtagcc actcctatct ataaatcatt
     3301 tattgatgaa gagagaatcg aggagtcgtt acaggatttt aataaatttc atcctatcgg
     3361 cagaatcggt aaggcaacag atatcgctga tgcaatcgaa tttcttttgt cagaaaaagc
     3421 aagctggatc acaggggaga ttatgaatgt cgatggaggg gtaatggcag gaagaaatta
     3481 atcagcaaac cttatctaga tttatgcctg cgagcatcaa tagcaaaaga ttgattttta
     3541 atgccctgtt tcagatttat ctgatgtggg ttctggataa ggattatata agaagcagtc
     3601 aatatccaga gtccgtttgg gctgaggaga tgcgtaagca tcgtctcgaa gccttggcaa
     3661 gaaacattaa attccataca aggcttcgag acggcataaa tgcctcctca gcccgaacga
     3721 ttcaaaataa tggctgtctt atccataact cacgttatcc ataaaaatca atctcttgct
     3781 attgatgctc gcactaaggc ttttcattgc ttgcatggaa taatgtggcc atttgctttt
     3841 ggcaaacatt gataaccatt acggtctaaa aatctcttgg gatccttgag aaatttcaaa
     3901 aaatactccc agattttttc tctctttttc gagaaagtcc atgaatccac tatatatttt
     3961 tttatcacca aaccagggat cggttacttc aaactgccca tggcgtttct tgcctaataa
     4021 ggtatggagt actccagctt ttataatgat aagagaggtt atcccctcag ggaaatctgc
     4081 ctcacttaag gttgtcaatg tcattttttt tcttattggc acctctttct taaaaagtga
     4141 atagagtgaa gtaataacca tatcttttga atatttattt cgtacagatt ttgatagctc
     4201 tctaatctcg acaccaatga cattcaaatt atatttgcaa aaataggatg cgatcttttc
     4261 aggcgatgcc actttaccgg gttctaacca aatgtctttg tatatttcca attctttgcc
     4321 aggtgtatag ctgcttggtt caataaaccc cctttcaaga agtattttca ttattgctct
     4381 tgcagtacaa gaattttcga atttttgtgc atcgaccaaa ggttcaatgc catccagttt
     4441 tgctcttaat ttaggattat ctaatacaaa atttgcaatt tcattagagg ttactattaa
     4501 ttttaagaag cttttttggt caagcatttg aacgaaatct tctgtggtaa gaagcaattt
     4561 aatcgtatca gaggagacat ttttcatctt ccccaaagta ataatccctt caatgggctt
     4621 atcgtgagtg ctatgaatac tcttaaattg attaataaat ttctccaagc tggttttatt
     4681 gatggctata tcagcatctt ccccaaaatt aaaatcaata ctttgataaa tattttgaca
     4741 ggatttaata gcattaagcg tcaaatctgg aaattgctct gccgtaatat tcaaaatcat
     4801 aataacctgt cttcacatcc caaaactgac actaacttga ttataggtta ttttgcatat
     4861 aaataattct ttttgatata taaaaatgca attgtatctt gtatttgcct attctgattc
     4921 tgttctcctt tcattaaatc ctggttagca aaaaagtgaa gcaatgactt aatattcagt
     4981 taataaacat agtgtaaaat tattaaactt ttaatcatca ctatttgctg ctatgaaaaa
     5041 gaattttttt tctgatcttc ctgaggaaac aattgtcaat acattgagtt tcttaaaagc
     5101 aaacacacta gctcgtatag ctcagacatg tcaatttttt aatcgcttgg ctaatgataa
     5161 acatctggag ctgcatcaac taagacaaca gcatataaag cgagagctat ggggaaatct
     5221 tatggtggcg gcaagaagca ataacctgga agaggtcaaa aagattctaa aaaaaggaat
     5281 cgatccaacc cagaccaata gctaccactt aaatagaacg cctttacttg cagctatcga
     5341 aggaaaagca tatcaaactg caaattacct ctggagaaaa tacactttcg atcccaattt
     5401 taaagataac tatggtgatt cacctatctc tcttcttaaa aagcaactgg caaatccagc
     5461 cttcaaggat aaggaaaaaa aacaaatacg cgccttaatt aggggaatgc aagaagaaaa
     5521 aatagcacag agcaagtgcc ttgtttgtta aagctgttaa attaaacaga tcgcgtctgg
     5581 tctatgatag atgtgatttt ttttccagca agtgtttgca gcaataaaca aactcattaa
     5641 attgagagtc ttttacaaaa tatcctgcta tatccaaacc tgttgttgcc tgcttatccc
     5701 ggatattact tgatgtcgtg aagataacaa ccttaatcgg attataatga atatgagaac
     5761 gcaattctct gagaaattct ataccattca tttttggcat catgatatca aggatgatga
     5821 ttttaggctt ggtcgctttg atatttccag agagttgttc gagagcttca acaccatcac
     5881 gagctatatg aaaacaaact ggaatactca attttgtcag cgctctttga acagacatta
     5941 aatcgacttc atcatcatca accaataaaa catgggtaca ctcatcattc ttagcacgca
     6001 tcgccaatct ccttatcttt tggccataaa aaacgaaaga cgctgccttt gtttttttct
     6061 gattccacag aaattttacc tccttgaaac tcgactattt tcttagcaat gctcagacct
     6121 acaccacttg tttcaagttt gtccctgggt tgtaaggtct gaaataattg aaaaattctg
     6181 tcttgatatt ttttttctat tccagggcca tcatccgcta caaaaaactc ataaaaccga
     6241 tccttttctt ttgtaccaat gatgatatgt ccatctttac ggtgatgatg cctgatactg
     6301 ttatcgagaa gcgctgtaaa aacctctctc aaaggaagct cgcaagtatt caatactggt
     6361 agtgtatctt cgagttgaaa cgtgacagtc tgcggaggat tcaatttctt tatgatatca
     6421 gtaatcaatc tatcagtgtg aattgtacga accttatcaa aaacatatcc tgattgagcg
     6481 tatttaagca atccatcgag caactttgac attcgtgaag cacgttgacg caagagcagt
     6541 aaatattttt tagattgttc agaaagcatt tcacccagat cttcttcaat ccatgagatc
     6601 aaatgctcca tagcaggcag aggcgctttt aaatcatgtg atgcgtgata gacaaattga
     6661 tccagctcat cttcagcttt cttcaccaaa atcaaattag caactctggc tttaaattcc
     6721 ttggccgaga atggttttat cacataatcc tgagcccctt cttccaacat acgtgcagtt
     6781 aattcatcgt ctgctttggc agttaaaata ataattggag ttttcaatag cagggggtgt
     6841 tctctaatag catgcaccat ttcgacaccg ctcatatgag gcatcataat atcactaaca
     6901 atgagatctg gatgtaaagc aagcgatttt tgcaacccct cccgaccatc aaaagcactt
     6961 tcagtacgat aaaaagtgtt caatatttta caaataaaat gattcatctc ctgattgtct
     7021 tctactacca ggatcagcgg tctatcgtaa taatcctgtt caaaatactc ttcagccccc
     7081 tccttaattg gttttacttt atttctgaat tcagcaagac tcgcgcttat ttctttttcc
     7141 cccaataata gctggctata ggaagaataa acctttgcat tttgaggcgc ttttagtggt
     7201 aactcaatgg taaaaatggc accgccattt ctagcattcc ctgccaaaat tgaaccccca
     7261 tgtaactcaa cgaaatcttt tgcaatcgat aaacccaacc cagttccccc taccgtacga
     7321 gttgtagatt cctcaatctg aaaaaaacgc tcgaatatag tttcacaaag atcagaagga
     7381 atacccggtc cattatcttc tatctgaagc ctgcatgttt caggattgat ttgcactaat
     7441 ctaatcctta ctttcgcatt ttgtggtgtg aattttatgg catttgacaa aatattgatt
     7501 aaaacatgct ggattttgtc gggatctatt tgtgcttgca attcatcagg caaatccatt
     7561 gaaaaaacaa gcttgcgttc gggaatctga gcctcaaaca atgcaattgt ctgttgaatt
     7621 aattttacca aatcaatatt ggcataatga agaatcattt tgccagcatc cagctttgat
     7681 atattcagca aatcattgac atgttttagt aaaatttgag cattattttg tatgcgcctt
     7741 aatgtattct gaacaaatga gggaagcttt ttttcagcaa gcaacatttc cagaggatct
     7801 agtatcagca ttaatggggt acgaagttca tgactgacat tagcgaaaaa ttgggttttg
     7861 atttgatcaa gtctagccag atttttatta gcctcttcca actgcttgtt tgcttcctga
     7921 atttcctggg cacgctgata gatttcaatc tccatttccc cagccttggt acgcagttct
     7981 tccatcagct tcagctgcgc tgaaccagtt ttttttaagt ggacaaattc cgtaacatcc
     8041 tccacacgat gaataatata ttccacttcc tgatcagccc ctaaaaccgg agtattaata
     8101 gggctccaat aacgttcttc aaatccccct cctcgatctg caggtctctg aatatcatat
     8161 ttttgtatag ccattgtatc tgctgatttg ttttttagaa cccttagtag cgatgcatat
     8221 aggttcttag cacctgttgc ctttggatcg tttggattat caggaaaagc atcgaagaca
     8281 ttttttccca aaatttgttt gcgactcacc attgttgcct gtaagtaggc atcactgact
     8341 ccgacgatat taaaatccct gtccaataaa aggtataaat ccggcaggga atcaaatata
     8401 gatttaaaat ctatatttga tttcgtattt tcccttcgca tagagcatcc tccttcctct
     8461 atgtaaggcc tattgactat aaagcattac acaagtcttt gcgaggctgg ccatgaatcc
     8521 aatgtacaat tttcaacaca gggtatgaat agccaattaa ccctactact aattataatc
     8581 gaggatttga gaccttacta attaggaaag cctggtttcg ataattgctt gcaaccacca
     8641 acaaaaagat gaacctcttt cagccagtac aacgcttgct ttagtggttc gatcccctct
     8701 cagcaatttt tcttcaatag tcgccctatc gatttaagat cttatcgtca atcaaatata
     8761 taaaaataat acaaaaaaac acatacaaaa ccaaagtgta caaagtttat tttaatctac
     8821 aattaaatat agggtatcta tcagtgaggt taatatgaca acttccaagc ttgaaaaaac
     8881 gggtttgcat gttcatgaaa aaatcaaaca tatggtcaag aattatggaa cgatgataac
     8941 cggtattcct gccgaaatat tagggcaaaa tgaggcagaa atttcggttg gatatgtaaa
     9001 aaaaatggga aatatgaaag aaaatattgc tgaagtagtt cgaaaatctg aaatgactca
     9061 acccacaaac agctgcggaa aagcgagcaa tgaagtgtgt gatttacttt taggaacaga
     9121 aggagcgagc gaattcgaaa aatcaagcta tcaagtatta tctggggatg gcagtaattt
     9181 gaaaggttct ctacccaata aaaatttact ggtcagggtt gaaatggatc gatttaatgc
     9241 tcctcaaaaa taccaaaaaa taaaaagaga ggagtttaat cccgagaccg cggaaaaaaa
     9301 taaaatttat ctactggaag atcagctggt atatctcgat atatttggaa aagtcatcga
     9361 ccttggacag acatcagaca catgtcatag actatttaat gctattacca ccccttttta
     9421 tcaaaattat attctttatg atgagtatat tgatccggaa gaaagtgctg aggaagcggc
     9481 catgtttgaa atgggtgaga tagttaaagc taaaatgaaa aacatagatt gttggacagc
     9541 aacccattct tttactatat ttgttcctga atcggacagc gaagacacac gcacattata
     9601 tccttaccag gcatattgga ccagccatac tttgcaacaa tggttttccg gtgataaaga
     9661 tgaaaaatta tcacgcttgg gtatcgatgg ctatatcgaa aaactggctc tattaggcac
     9721 aacaaccgat agcaaaatac gctccagcat ttatggagaa ctattttctc ctccaggaaa
     9781 agaacatgtt ttttgtaccg ggatgaacga aaaattctcg ccattacgag tcaaatttaa
     9841 agtcactgaa gttaatccag aaatagcatt gcaaaattta gaagaagttc aagagtttat
     9901 tgatacgaat tatcctggcg aaaacgcgaa agatcaatgc gaactttata aaataaaagc
     9961 gcaagaagcg atgactaaac aacttgagat gcgacttcta atagagccga ctcatcgaaa
    10021 agaatcagta accccctata aagaaaaaaa cacacaatct ttctttagca aaaacttaga
    10081 tacaaccagt agaatagata aaatgatctc atcagtcatt acgatggaaa acctcaagat
    10141 tcttgcaaaa gaagcagata ccctctctgg aaaggatcgt gaaaagttag tggagtattt
    10201 taaaacatta ccatcagagc agctggcaga aatcaaggct gaatcagaaa cagctcaaaa
    10261 agcctgggat gaacaatacg ggttagttct tcgtgataag tgataaatcg aatcggcttt
    10321 gtgtatttgt cacttgaact gtttataatc aaatgatatt gacctataat atatcacttt
    10381 gattgtgcgt ggcattaata tgacaaaaat aaaactggaa tcgccgggtt tcatggtaca
    10441 caaaaagttg aaaagcatgt cccaaagcta tggagtcatg atgactggtg tccctgctga
    10501 ggtgttagga caaatgcagg cagagcgatc tatccccagc attaataaaa cagggaacct
    10561 taagcaacaa attgcaaagg aggtttcaaa ggtatgtcat atgatgactg aacccactca
    10621 gagttgtggc caagccagta atgatgtgtg tgaattgctt ttaggaaaaa ttgaagcgga
    10681 aaaattccat ttcaccaaat atgaagcatt atcagcagat ggagacaatc taaaaaatgt
    10741 tttagaaaat acagccccat ccagcactaa cctattaata cgatttgaaa ttgacagaga
    10801 agaccctccc attgtattgg tcaaaacaaa aaacgagaat ttcaatccgg aaaccgctgt
    10861 gaagaataaa atttacctat tagaaaataa attatatttt attgacaaga tgggtaatct
    10921 ttttaacctt ggaccaggta aaaaaaaatg tacgcagtta ttcaacgcaa ttggagactc
    10981 agcagaatat agtctttgtg atccttttgt tcttgaagag cctgaaaaac ctgaggattt
    11041 tgcaataagt gagatcgtag atatctttaa cgaacaaaaa gagagattcg atttttggat
    11101 cgggtcgcat tcttttacaa tatatatccc tcaaacgctg ggtgaatcac cacgccaatt
    11161 ctatccttat caagcttact ttggttcaca cactttacag gattggtttg tcagcgataa
    11221 agatgaatac ctgtcgcgaa ttggcatcga caagtatatt gaaaaattag ctgtactggg
    11281 aaaaacaaca aacacgaaag aacgctcgga tatttatgca gaattttttt caaagcgagg
    11341 ccgggaagca tttttttgcg cccaccttaa tgaaaaaaga caaccattac gagtaaagtt
    11401 caaaataact gaaattaacc ctgaattggc attaaagaat ttacaggaaa cgcaagaatt
    11461 tattgatacc catccaggag aaaatccatc agataaagta gaaaattata gaaatagagc
    11521 aaaattggca atgaccgaac acctggagag cctattagac atcaagcctg aatcaagccc
    11581 ctctttattt tggaaaaatg tcgataagaa agaacaagaa atgactgaca gtagcaggta
    11641 taaaaagggg ccaagctaat caataaaaca ataaaaatca attaaattat aaattgttct
    11701 aaaattgtat taatgggtgt ttgaaaataa cgaaggagtt tgatttatga tgagtcgcga
    11761 aaaagcagct ggaacttttt ttaaagatta ccagaaaaaa aatgtaatga gattgttaca
    11821 agactctctc gaaaaaatta tcaacgaatg gctgaaaact gatgatgaaa gccataccaa
    11881 actgaaaagt cttcaagaac tttcagagat ggatattaat gctacctcat ttgctgaaca
    11941 ctcaccactt cctgattttg ttacgcgtct ctggctggat ccacataaag cgcttgatgc
    12001 catggacaaa aacatttcaa aaaacgaaat cagaaagctg attaaagaaa cggcgaggga
    12061 aatagaactt gtctttactc atcaaaaagc acccgactac tcttaattga aatgaggatg
    12121 gggctggtat attaattctt cattcatttt ggccatatca ttggccaaat gatgtcttat
    12181 tggataaaaa catacacaga aaaacaattc gaaaattgac taatatctaa aaacccttct
    12241 aattaatttg ttatgttaaa ttaagtctat ggaaccgggc ctccagaggt tcgttctttt
    12301 ccaccacaaa ttactttaaa tataaggggt taaaagataa tgtcatgcta tattttggat
    12361 ttaatcgaaa cattaaaaaa tgattttgta aaaaaaaata cccctacaag atttgacagc
    12421 gtcaaatctt atggagagtt attggaaaaa tcaaaaatta atcccgcgat tgcagagtat
    12481 atcacaggca cttcatccgg ggttatgatg gttgattcct atgaaaaact attagaactc
    12541 tctaaaatta actatgctgt tgctgataat atgacacaaa ctcaattggg tctggattta
    12601 attgatagct atgaaaaatt tctggaaata acaaagataa atgctaaagt tgcttttaat
    12661 tttcaaatat taaattatca aaggtatgtc aaactggtcg actcatatca acaatcttca
    12721 caagcaaaat ctctttcaga cctaaatacc tcggcaaatt cgcaactttt ttttaagaaa
    12781 cgcgattcta ctggacaaga ccgagcttct attaccacag gacaagcgag agtagctcat
    12841 gatacagttg gattggggct gaaatggaac gaagctatcc agaataccat tagcgagaac
    12901 atgatgaacc agagacttta acagcaaacc ctaatagcta ttctagaatt taaatttaaa
    12961 aaggccataa tccagaaata tgaaaaatcg acgtgatgaa caaacaatgc ttacactcat
    13021 catcaaaata gctaatgacg atgagaggat taaaattgtg attatgaatg gctctcgagc
    13081 aagcccttca gcgaaaaaag atatttttca ggattatgat attgtgtatc tggtaactga
    13141 aatagaatca tttgttcatg ataaaaattg gattaatcag tttggtgaat tacttatcat
    13201 gcaaacacct gatgaaatgg atgggcaatg gccaaaattt aaaggtaaat atacttatct
    13261 gatgcaattt aaagattgga ataggattga tttaaccttg ttacacatcg atcaactgga
    13321 aactatgcct agagacagtc aaagtctctt attgcttgat aaagacaatt taattgcacc
    13381 ttttggcgaa cctactgatg aagattactt accaagcccc cctacagaaa aagattttgt
    13441 taactgttgt aatgaattct tatgggtaag cacttacgtt gccaaaggac tctggagaaa
    13501 acaactcatc tatgcaaaac acgtttcaga gcagatagtt aaagaagagt taattaaaat
    13561 gctcatgtgg tatgcaggaa ttcaaaccaa ctttagtcaa ccgatgggaa cttatggaaa
    13621 atatattgaa aaccaccttg agctcgagct ccggcaagaa ttcctcaaaa cctacgttga
    13681 tgctgattat aagaacatgt gggcttcatt attcaaaatg tgtgaaatat tcaatgacct
    13741 agcgataaaa atttcaagtt attttggtta ctcatttaat cagaaagaat tcgataatgt
    13801 ggtagattat ttgcaagatg taaaaaacaa taaaattcca acttaatcga acaaatccac
    13861 ttggagttaa agaataaata ctattgagat atcgatgttg gttatcgatt tttgcaaatg
    13921 aattgagagc actccgccat atgaggtgga acaaatttta aagtttgaag gaaatgttat
    13981 gcccaaaact ccaaacaatt gtagtttatc cagcggtaat aaaccaccaa ccttcttaag
    14041 aatcttgttc tcatatatgg ctgaataccg ctggaataca gcatttttta ttttatttgg
    14101 ccttttttgg gcatttactc taccttatat gtcctactta cttgggcaaa tcatagatga
    14161 aataaaattt caaaatccta aacagttcga cacctttagc ttaatcctaa cctcattaat
    14221 tctttatgtt gcaattcatg ttttgcgaag cttgggatat tacgttcatg gtttgttttc
    14281 acttaccagt attcccgaaa aaaaagccaa actgattaaa caactattta atcacctggg
    14341 caatcaatcg atcggttatt ttgaagataa gcactccggt tatttaacaa acaaaattac
    14401 caatgccagt tctagtattg agccacttat ttttaattta tttaccatta tttacccaca
    14461 atcattagca attatcctaa caggtatatt attgtcctta gtcgttccgt attttggagt
    14521 ggttctctgg ctctggggag ctatacttat tatttattca taccattccg ccaaaattgg
    14581 taatgataaa gcaatgctct ttgcgaattc caatagtaaa gtcaacggta aggtagtcga
    14641 tgtggtcaat aacatccaca cagtaatcta caatgctgaa atgtcttttg aacttggtaa
    14701 tctggataca gacattcagg atatggttca aaatgatcgc aatttgcagc gccacatgac
    14761 aaaagtcatg ctggtacagc acattgcaat gaatatcctc gtcgctttct ttttaatagg
    14821 ctcagtgctt gggtataatt atggttatgt cagtgtaggc gacattgttt ttgttatgac
    14881 atcagttact gctattgcag gcctcacaag cagccttggg aaatgctttc tcgattttgt
    14941 tactaatgcc gggcgtttaa atgaagggtt gagcttattg ggagatcaac atacgattac
    15001 tgataacgca aatgctcaag cacatcaaat taataatggt gaaattcaat ttcatgacat
    15061 ttcatttgct tatcccaatc aaccgccaat ttttctaaat tttaatttaa cgattccagc
    15121 aaagcagaaa ataggaattg taggtagttc aggggctggt aaaacca
//
LOCUS       DACTJE010000071        15144 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.071, whole genome shotgun sequence.
ACCESSION   DACTJE010000071 DACTJE010000000
VERSION     DACTJE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15144
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.071"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..204
                     /locus_tag="I9760_001675"
     CDS             <1..204
                     /locus_tag="I9760_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043444.1"
                     /translation="NSSSSSSSDTSSQLSPPPDSNTNTSTPDLGSQTKYDETSSSSNS
                     GSGQSSGSGSSSDVPTPSQSMPG"
     gene            complement(235..1248)
                     /locus_tag="I9760_001676"
     CDS             complement(235..1248)
                     /locus_tag="I9760_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine monophosphate reductase"
                     /protein_id="HAT5043445.1"
                     /translation="MTDQAITFDDVLLVPSYNHHESRRVVETTSTDRLGKLTLNLPVI
                     SANMDTITESNMANFMHSKGAMGALHRFMTIEENIQEFKKCKGPVFVSVGCTENELQR
                     AEALRDAGADFFCVDVAHAHAKYVGKTLKSLRQLLGSRCIMAGNVATYAGADYLASCG
                     ADIIKAGIGGGSVCSTRIKTGFGVPMLTCIQDCSRADRSIVADGGIKTSGDIVKALAF
                     GADFVMIGGMLAGSAPTPGEVFQKDDGSKVKRYRGMASREAQEAFLGQMHEWKTAEGV
                     ATEVPFKENPDGIIADIIGGLRSGLTYAGADSISELQRKLNYVIVTQAGRIESLPHKL
                     LEG"
     gene            complement(1271..2677)
                     /locus_tag="I9760_001677"
     CDS             complement(1271..2677)
                     /locus_tag="I9760_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAT5043446.1"
                     /translation="MDNTNTGLKLFVLDTNILMHDPTAIYHFDEHDIYLPMVVLEELD
                     SHKTGTSELARNVRQTNRMLVELMSNATHDQIVSGLPIPSYINSSNKKCSGRLFFQTD
                     EFDQVVPSTLPGHKVDNTILATALGLQKKYYGKRQVIIISKDINLRIKAGILGIHAED
                     YYNDQVLDDVNLLHRGLHILDNNFWDSHAKDMGSWQESGKTFYKVSGPLVSQWNPNDC
                     LSTEDDQFQALVKKIDGPNAVVQLIRDYTQPKHSVWGITARNREQNFSLNLLLDPDID
                     FVSLQGSAGTGKTLLTIAAGLTQVMDQNRYSEILMTRVTIPVGEDIGFLPGTEEEKMT
                     PWMGALMDNLEVLHSSQVGGSFGRGATQDLLQNKIKIRSLNFMRGRTFLNRYIIIDEA
                     QNLTSKQIKTLVTRAGPGSKIICLGDIKQIDTPYLTETTSGLTFAVDRFKSWEHSAHM
                     TLTRGERSRLAFYAAEHL"
     gene            complement(2832..4031)
                     /locus_tag="I9760_001678"
     CDS             complement(2832..4031)
                     /locus_tag="I9760_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HAT5043447.1"
                     /translation="MFIRNLQLFIVVASCILVLIGTDILLPSLPNIAHALSVSSNEAK
                     MLISIFMIGQFSTVLIWGVIADRLGRRKTLFLGMLIFFIGSIFSLKADSIQLLLACRF
                     LQGTGAVVVPVAGWALVQDLFPKDEGARIMTWIGTLTAVIPLFAPALGGQLDVLYGWR
                     SNLYCIAFYSFILCVIMLFLPRTIPVQKTTTADLKETLSIYIRIIKNKTFISYIALFG
                     LLNCGEWCFLTVAPFYYSHAHIAADTMGILLMITSMGFVCGSLLASRLFNRLGIDKII
                     ALGIQLAVMSSLMLLIGEYFHWSKYQLYNAITIGVYILSSALLWGATTSRALQCFDDC
                     RGSASAIRSLILLCFAAFGTYSGRLVNHNSLFPVGLFLLFMAFIAFIVFNNKELKTER
                     HMEGIPI"
     gene            complement(4157..4621)
                     /locus_tag="I9760_001679"
     CDS             complement(4157..4621)
                     /locus_tag="I9760_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAT5043448.1"
                     /translation="MKIGEMVPDFAFNATNNVQGHLNDYKGQIIVLYFYPKDATPGCT
                     TEGQDFRDAYSQFKDLNTVIFGISRDSLKSHEQFKSKQNFPFELISDSNETLCQMFDV
                     IKMKSMYGKQVRGIERSTFIIDTQGVLTHEWRKVNVKGHVEEVLDTVRSLAG"
     gene            complement(4633..5103)
                     /gene="smpB"
                     /locus_tag="I9760_001680"
     CDS             complement(4633..5103)
                     /gene="smpB"
                     /locus_tag="I9760_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAT5043449.1"
                     /translation="MTTKKQPDSTIALNRKAGFDYFIEDQYEAGLVLEGWEVKSLRAG
                     KINLSDSHVIIKYGEAFLLGAQIQPLPTASTHFIPDPIRTRKLLMNKKELNHLIGSVE
                     RQGYTIVPLSLYWKKNKIKIKIALAKGKKEHDKRDTIKDREWQRDRSRIMKKNT"
     gene            complement(5215..5979)
                     /locus_tag="I9760_001681"
     CDS             complement(5215..5979)
                     /locus_tag="I9760_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAT5043450.1"
                     /translation="MKDIIIASFYKFIPLNDFESLREPILTKMHEIGIKGTIILAHEG
                     VNGGFAGNREQMNVFYDYLRSDSRFADLHFKETYDNKNPFDKAKVKLRKEIVTMGVQK
                     VDPSYNAGTYLSPEEWHQFIQDPNVILLDTRNDYEYELGTFKNAINPDIENFREFPDY
                     VQRNLIDKKDKKIAMFCTGGIRCEKTTAYMKELGFEHVYQLHDGILNYLESIPESESL
                     WEGKCFVFDDRVAVDQKLDRVYPQLPQDYKYEREQK"
     gene            6232..7533
                     /locus_tag="I9760_001682"
     CDS             6232..7533
                     /locus_tag="I9760_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAT5043451.1"
                     /translation="MIQNNRICRWLFIVLLSLPLVGFTSIPVKSIVVFGDSLSDNGNT
                     THLLKSLRQEEDPAFLVAPFKIFVINKMIEFANDYYVPQIVLDAGISAVTDFFDHDLA
                     PYLANLVGKVKQVPVLPGKPYWKNRFSNGRVWIEYLAEMLSIQKSDEEVYLNKAFGGS
                     WSATYDYQLTVWNLIRHPLGTIKNLIVGKLIPPSLGLIVQAYLLEHEQLSDETLYFIY
                     SGSNDYINVLFFEDNYNTEVMSTYIDNVLDGLSSAVLKLANAGARRFVIMGIPHVGDT
                     PKFVKTTDRDVLNAAVDLHNERLAKRIDAWKERYPTADFLFVNTEQYLKKAFEDPEKY
                     GFYNVKDACIDVKFPMFNAFIHSPFARNYVLQYTQVLQYRDKQFAPGENNYHMCSEPE
                     DYLFWDEIHPTTRAHNYLAYEVCMALEEHGYEVTCKMPESA"
     gene            7897..9711
                     /gene="glmS"
                     /locus_tag="I9760_001683"
     CDS             7897..9711
                     /gene="glmS"
                     /locus_tag="I9760_001683"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAT5043452.1"
                     /translation="MCGIMGAVSERDISKILLEGLRRLEYRGYDSAGIAVIDSQDRLK
                     RVRIQGKVQNLADAMQETAIAGNTGIAHTRWATHGKPSEQNAHPHLSHGEIALVHNGI
                     IENHEHLRQQLITYGYQFTSETDTEVAAHLIHYHYQQHENLLLAVQKAAAEMQGAFAL
                     GVIHQKRPEELVAIRKGSPLVLGFGIGENFIASDALALRSFAQSVIYMEEGDSACVTT
                     QDIKVYDSNRILVQRAVHPLNSDSEIVNKGPYRHFMLKEIFEQSKVITDTLESRINSI
                     DVLRASFGEKASHIFPVVKNIHIVACGTSYHAGMIAKYWLESLAGLPTQVEIASEYRY
                     RDVVVPDNTLFITVSQSGETADTLAALFKAKQSNYLASLAICNVATSTLVREADCVFL
                     TRAGIEIGVASTKAFTTQLAAFLMLAAALCKDNRAQEVLRQLQELPACCERVLQMNEE
                     VESLASLFVNKVHALFLGRGVQYPVALEGALKLKEISYIHAEAYPAGELKHGPLALVD
                     KDMPVIAVAPNDELLDKLKSNLHEVSARGGQLFVFVDDSQNWKANGARLIKVPSCGAW
                     LAPIVYTIPLQLLAYHVAVAKGTDVDQPRNLAKSVTVE"
     gene            9708..10373
                     /locus_tag="I9760_001684"
     CDS             9708..10373
                     /locus_tag="I9760_001684"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HAT5043453.1"
                     /translation="MNKGQYFWNELWCEGRISFHKEEVNPDLIAYISSLNIPAKGRVL
                     VPLCGKSVDMLWLVRQGYHVVGIELVEKAILQFVQEHQITVRENTTGQAKQYFTDNLN
                     LWITDIFALNAALIEPVDAIYDRAALVALPKKLRPAYVDICLKWLKPGGSILLKTLQY
                     NQEKVQGPPYSVSPEEIALSYQQCAKIELLKSQKRIREPNDHLFNLGISEVNDYVWRI
                     RKG"
     gene            10497..12866
                     /locus_tag="I9760_001685"
     CDS             10497..12866
                     /locus_tag="I9760_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAT5043454.1"
                     /translation="MAEVMLASAAIIADELKVKVSQVETAIRLLDEGATVPFIARYRK
                     EATGGLDDVQLRFLAERLIYLRELDERRTVILQSIREQEKLTPELEQSILAAETKTRL
                     EDLYLPYRPKRRTKAQIAIEAGLEPLAHALWKDPALDPEEQASQFINSEAGIENTQAA
                     LDGARHILMEHFAEDAELINELREYLWQHGIVKSVGNSTKKDQANKFSDYFDYSEAIK
                     KIPSHRALALFRGRREGILQVSLVLPDQDANYGENKVAAYFNVTDRQRKADGWLIDTV
                     RMTWKIKLFTKLELELLTRLREIADEEAIKVFSKNLRDLLLAAPAGPQVTIGLDPGIR
                     TGVKVVVVDATGKLLDYSVIFPFAPQNEWHQSIADLAKLAAKYQVNLISIGNGTGSRE
                     TERLVADLIKMYPDLKLTKIIVSEAGASVYSASEIAANEFPDLDVSLRGAVSIARRLQ
                     DPLAELVKIEAKSIGVGQYQHDVNQAKLARCLDGVVEDCVNAVGVDINTASAALLSHI
                     SGLNDTLAKNIVQFRDEHGAFTNRNQLKNIHRMGEKAFQQAAGFLRIMNGDNALDASC
                     VHPEAYPLVEKIISDKKVSIKEIIGNRELLQSINAEQYVDENFGLPTIRDVLRELEKP
                     GRDPRPEFRTAQFQEGVEDIKDLEQGMVLEGVVSNVTNFGAFVDIGVHQDGLVHISAM
                     TNRFITDPRSVVKAGDIVKVKVVEVDKERRRIGLSMKLNDEQAPAVLQKANKPQPARK
                     QAEQKQPEFKKKQEHKKKPDKIKKSEPVKKTVFNTAMADALAKLKRGGE"
     gene            12869..13249
                     /locus_tag="I9760_001686"
     CDS             12869..13249
                     /locus_tag="I9760_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAT5043455.1"
                     /translation="MQTPKGELTIQTLAMPLNTNANGDIFGGWIVSQMDLAAGVLAKR
                     ISHGRVATVAINSMTFLKPVHVGDVVSCHVELVKVGNTSMTIRVEVWAYSSIKMEKYQ
                     VTEGIFVFVAIDENGKPRQVPKTQ"
     gene            13246..14886
                     /locus_tag="I9760_001687"
     CDS             13246..14886
                     /locus_tag="I9760_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2832 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043456.1"
                     /translation="MTNKTQELQRVIADMAVLIEQDAEPDPQLYAFFFQHPEISLELI
                     HLINNLSEELVHDGPPIYSACVFALDICIAQLQAAAEANNKNATKLLSQLMNHLAEVI
                     DTHKHSLSFWLPVLNAFYEVHVELTDDLKEAYYGLASQDDDAVEVDEQFNHLDSIKDL
                     IHELSDLTLFDIAESFFAQSYAMPPDFFADLIVDLYSIEEGKDIALLVLLHPNIEVRE
                     IALSTIDQLINTITLTPASLSRLQIIKYWYPTHYHGMFDRWIKTQRKKGVTFETEASQ
                     AKITIKATEVDGSGSQGVFIHARKNKKNRLCGLLLKYDLGIKDAWITPEISAAEVTDY
                     YHKAFEDSVTLREVDFSYFQTIVEHFLAITVEKSDIPNLHFLEILEFLGIRLKPDKLD
                     LDCVFEQLSVQIIPFTQEKIAESLKRSKMWLKNKQFTESWYIENPTIDKIVNHNSSFV
                     EGVKVCRLADAIDAVFSEEMELHRDKWQFHFLWIALWVKAKHKKNEKVWQDSFLIAYA
                     IHQGYPLKDIPVMKEICYQTVVNSIETMQERRTHLSQE"
     gene            complement(14941..>15144)
                     /locus_tag="I9760_001688"
     CDS             complement(14941..>15144)
                     /locus_tag="I9760_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VipD"
                     /protein_id="HAT5043457.1"
                     /translation="DFKVEVQPEPVKVSTSENQETVSNQKDINPAVEYRKIIAEVRRE
                     HTDPSPSLQEKERVGLSTTFGGH"
BASE COUNT         4531 a         2792 c         3228 g         4593 t
ORIGIN      
        1 aacagtagca gtagcagcag tagtgatact tcatcacaac tgagcccgcc accagactcg
       61 aatactaata caagtacgcc agatcttggt agccaaacaa agtatgacga aacatccagc
      121 agttctaaca gcgggagtgg tcaatcttct ggctcaggct catcttctga tgtcccaaca
      181 cctagtcagt ctatgcctgg atagatgtga aaacagatta tccgggttgg tgagttatcc
      241 ctccagcaac ttgtgaggca agctttctat gcgtcccgcc tgggtgacaa tcacataatt
      301 caattttctt tgcaattcgc tgatggagtc ggcgccggca taggttagac cagagcgcaa
      361 tccgcctatg atatcagcaa taatcccatc agggttttct ttaaatggca cttcagtcgc
      421 aacaccttct gctgttttcc attcatgcat ttgtccaagg aatgcttcct gggcttctcg
      481 ggatgccatg cctcgatagc gtttgacttt gcttccgtca tctttttgaa agacctctcc
      541 tggcgtgggg gcagagccgg ctaacatgcc gcctatcatc acaaaatcag cgccaaaggc
      601 caatgctttg acgatatcac ccgatgtttt gatgccgcca tcagcaacga tggaacgatc
      661 ggcacgtgag caatcctgaa tacaggtcaa catggggacg ccaaagccgg ttttaattcg
      721 ggtgctgcat actgagcctc ctccaatacc tgctttaata atatcagcgc cacaagacgc
      781 aagataatct gccccggcat aagtggcgac attcccagcc ataatgcagc gacttcccaa
      841 taactgacgt aagcttttta atgttttgcc aacgtatttg gcgtgggcat gcgctacatc
      901 aacacaaaag aaatcggctc ctgcatctcg cagtgcctct gctcgttgta attcattttc
      961 agtacatcca accgatacaa agacaggtcc tttgcatttc ttaaattcct gaatattttc
     1021 ttctatggtc ataaatcggt gtaaagcgcc catggctcct ttgctgtgca taaaattagc
     1081 catattactt tcggtaattg tatccatatt ggcgctgatt accggaagat ttaaagtcag
     1141 tttacctaac ctgtcggtac tggttgtttc aacaactcgc ctggattcat gatggttgta
     1201 cgatggaacg agcagaacat catcaaaagt gatcgcttga tcagtcatta ttaatcctca
     1261 taaacaaata ctataaatgc tcagccgcat aaaaagccag ccttgagcgc tctccccgag
     1321 tcaaagtcat atgagcgctg tgttcccaac ttttaaaccg gtctacggca aaagtcagtc
     1381 cggaagtggt ttcagttaaa tagggggtat caatttgctt aatgtccccc agacaaataa
     1441 ttttactacc tgggcctgct cttgtaacca aggttttaat ttgttttgat gtcagatttt
     1501 gcgcttcatc aattataata tagcggttta aaaaagttct tccccgcata aaatttaacg
     1561 agcgtatttt aattttgttt tgcagtaaat cctgagttgc cccgcgtcca aaactgccgc
     1621 cgacttgcga gctgtgaagc acttcaagat tatccattaa agcgcccatc cacggagtca
     1681 ttttttcctc ctcggtaccc ggcaagaatc ctatatcctc gccaacagga atcgttaccc
     1741 ttgtcattaa tatctcgctg tatcgatttt gatccatgac ctgggttaat cctgcggcta
     1801 tggttaatag tgttttccct gtacctgctg agccttgcaa gctgacaaaa tcaatatcgg
     1861 gatccagtaa caagtttagg gaaaaatttt gttcacggtt tcttgccgtg attccccaga
     1921 ctgaatgttt gggttgagtg tagtcacgaa tgagttgaac tacggcatta ggcccatcaa
     1981 ttttcttgac caaagcttga aactggtcat cttccgtact aaggcaatcg tttggattcc
     2041 attggcttac caaaggaccg cttaccttat aaaaggtttt accactttct tgccaggatc
     2101 ccatatcttt ggcatgacta tcccaaaaat tgttatcgag aatatgtaat cctctgtgaa
     2161 gtaaattgac gtcatccagc acttggtcat tgtagtaatc ttcagcatga atacctaata
     2221 tccctgcttt aatacgcaag ttgatgtctt ttgaaataat gataacttgt cttttgccat
     2281 aatatttttt ttgcagacct aaagcggttg ccagtattgt attgtctact ttatggcccg
     2341 gcagagttga aggaaccact tgatcaaatt catctgtttg aaaaaacaat cgtccactgc
     2401 atttcttgtt gctgctattg atgtaagatg ggataggcag tcctgagaca atttgatcgt
     2461 gagtggcgtt actcattagc tctaccaaca tgcggttcgt ttgccttacg tttctcgcca
     2521 attcggatgt ccctgtttta tgactatcca attcttcaag caccaccatt ggcaaataaa
     2581 tatcatgctc atcaaaatgg tagatagcag taggatcatg catcaaaata ttagtatcga
     2641 gcacgaacag cttaagtccg gtattggtat tatccatagg ttcaccctgt cagctaaagt
     2701 attcttcaat tcaagtggaa tcagtctcct gcgtaggcag ggagtacatt gaaatccctg
     2761 aattctatgt tcattgtgct gagctatggt ggttccgtca agaattatat tattaagtgt
     2821 ctgtttttaa gttaaatggg tatgccttcc atgtgccgtt ctgttttcaa ttctttatta
     2881 ttgaaaacaa tgaatgcaat gaaggccata aaaagtaaaa acaatccaac agggaacagg
     2941 ctattatgat taaccaggcg acctgaataa gtgccaaaag cagcaaagca cagtaatatt
     3001 aaactgcgaa ttgcagaggc tgagcctctg caatcatcga agcattgcag ggctctggat
     3061 gtggttgcgc cccatagcaa ggctgagctt agaatataga cgccgatagt tatggcatta
     3121 tagagttggt acttgctcca gtgaaagtat tctcctatta acaacatcag gctgctcatt
     3181 acagccaact gaattcccaa tgctatgatt ttatcaatcc ccaggcgatt aaataagcgg
     3241 gaggccagta acgatccgca gacaaatccc atggaagtaa tcattaataa tatccccatg
     3301 gtgtcagcag caatatgtgc gtgcgaataa taaaatggag ctacagtcag gaagcaccac
     3361 tcgccacagt ttaataatcc aaatagtgcg atataggaaa taaatgtttt gttttttata
     3421 attcgaatgt aaatgcttag tgtttccttc aggtcagcag ttgtcgtttt ttgcacgggg
     3481 atagttctgg gtaaaaacag cataatgacg cataaaatga aagagtaaaa tgcgatacaa
     3541 taaagatttg aacgccaacc atataacaca tccaattgtc cccctaatgc gggggcaaag
     3601 agtggaatca ctgcagtcag tgtcccaatc caggtcatga tgcgagctcc ttcgtctttg
     3661 gggaagagat cctggactaa agcccaacct gcaacaggga caacgactgc gccagtacct
     3721 tgcaagaatc gacaagccag taacaactga attgaatcgg cttttaaact gaagattgag
     3781 cctataaaga aaataagcat ccccagaaat aatgtttttc ttctgcctag ccggtcggca
     3841 ataacgcccc aaataagaac tgtagaaaat tgccctatca tgaaaatgga aatgagcatt
     3901 ttggcttcat ttgatgagac agacagtgcg tgagcaatgt taggcaggct tggtaacaaa
     3961 atatctgtac caattaaaac cagtatgcaa gaggcgacaa caataaaaag ttgcaggttg
     4021 cggataaaca tattgaactc ttaagaaaag ggcgtatttg atgtatttat ccgcaacaat
     4081 aatagcaaaa attaattaaa tactctataa aaagtatttt tttaaagcat caatccctat
     4141 cagggattat tcttatttat cctgccaaag atcgcaccgt gtctagaact tcctcaacgt
     4201 gcccttttac attcacttta cgccattcgt gtgtcaagac accttgtgta tctataatga
     4261 aagtgctccg ctctattcct ctgacttgtt tgccatacat ggatttcatc ttgatcacat
     4321 caaacatttg gcataatgtt tcattgctat cactgattaa ttcaaaagga aaattctgtt
     4381 tgcttttgaa ttgttcatga gattttaaac tatctctgga aataccaaaa atcaccgtat
     4441 ttaaatcttt aaattgtgag taggcatctc tgaagtcttg accttcagtc gtacagcccg
     4501 gagtggcatc tttagggtaa aaataaagta caatgatctg tcctttgtaa tcattaagat
     4561 gtccttgcac gttgttcgtg gcgttaaaag caaaatcggg gaccatctca cctattttca
     4621 taaaccatct cctcaagtgt tctttttcat tattcttgaa cgatcccttt gccattctct
     4681 gtctttgatc gtgtctcttt tgtcatgctc tttttttcct ttagccagag caatttttat
     4741 tttaatttta ttttttttcc aatacaaaga aagagggact atggtatagc cttgcctttc
     4801 aacacttcct atgagatggt ttaattcttt tttattcatc aatagcttgc gcgtcctgat
     4861 cggatcagga ataaaatgag tggatgcggt gggaagaggc tgtatttggg cgcccaatag
     4921 gaatgcctca ccgtatttga ttatcacgtg tgaatccgac aaattgattt ttccagcacg
     4981 cagacttttt acttcccagc cttccaaaac caggcctgct tcgtattgat cttcaataaa
     5041 gtaatcaaaa ccagcttttc tattcaaggc tatggtggaa tctggttgtt ttttggtagt
     5101 cataattcat ctgatttgtt gaaaaaaaca gtataactga tgtactatcc cattgaactg
     5161 cttttcttaa ttttgttgcc ttgaacggct tatggcaaaa aagtatattc tacgttattt
     5221 ctgctctctt tcgtatttat aatcctgagg taactgtgga taaacacgat ctaatttttg
     5281 atccactgca acgcggtcat caaaaacaaa acatttgcct tcccaaagtg attcgctttc
     5341 aggaatagac tccaggtaat ttagaatgcc atcatgaagt tggtaaacat gctcaaagcc
     5401 cagctctttc atataggctg tcgttttttc gcaacgaatt ccgccagtac aaaacatggc
     5461 tattttcttg tcctttttat caattaaatt gcgctgaacg tagtcgggaa attccctgaa
     5521 attttctatg tcaggattaa ttgcattctt gaaagtaccc agttcgtatt cataatcatt
     5581 gcgggtgtct aataaaataa cattaggatc ttgaatgaac tgatgccatt cctcagggct
     5641 tagataggtc ccagcattat aagaggggtc aactttctgg acgcccatgg tcactatctc
     5701 tttacggagt ttcactttcg ctttatcaaa agggtttttg ttgtcatagg tttctttaaa
     5761 atgcaggtcg gcaaagcgcg aatcagaacg caggtaatca taaaaaacat tcatttgttc
     5821 tctattcccg gcaaatccgc cgttgacgcc ctcatgggcc aggatgatag ttccttttat
     5881 gcctatttca tgcattttag tgagtatggg ctctctcagt gattcaaaat cattgagagg
     5941 aatgaactta taaaatgatg caataataat gtctttcacg ctgtaaccta aagattaaca
     6001 aaaaatcaat tcgcaaaaat tatcatttac gcacaattta atcaatagga atgaattaaa
     6061 tgcatttcct ggacatcggg attttaacgg catattgggt gaaaaatagt tgcgttattg
     6121 catgttagta cttaagtata aagaatcgtt gacaataatt aaattctgat catactctaa
     6181 gggttaagct gctatctttt gtttcgctgg ctttgatata aggagatttt tatgatccaa
     6241 aacaacagga tttgtcgctg gctcttcatt gtattgttgt ctctcccttt ggttggtttt
     6301 acgtctattc cagtgaaatc aatagtggtg tttggagaca gcttgtctga taacggtaat
     6361 actacccatt tacttaaaag cctgcgtcag gaagaggatc ctgcgttttt agtggctcct
     6421 ttcaaaattt ttgttatcaa caaaatgata gagtttgcta acgattatta tgttccacag
     6481 atcgtgcttg atgcaggaat atcagcagtt acagattttt ttgatcatga tttggctccc
     6541 tatcttgcca atttggtggg taaagtaaaa caggtcccgg tgctgcctgg caaaccttat
     6601 tggaaaaatc gcttttctaa tggtcgtgtc tggattgagt atttggcaga aatgttatcg
     6661 atacaaaaaa gtgatgaaga agtctaccta aataaagcat ttggaggtag ctggtctgcc
     6721 acttatgatt atcaattaac agtttggaac ttgatacggc atccattggg aacaatcaag
     6781 aacctgatag taggaaaatt aattcctccg agtcttggtc tgatagtgca agcttatctg
     6841 ctggaacacg agcagttaag tgatgaaacg ctgtatttta tttattcagg aagtaatgat
     6901 tacattaatg ttctgttttt tgaagacaat tacaatactg aagtaatgag cacctatatt
     6961 gacaatgtgt tggatggttt gagttctgca gttctgaaac tggctaatgc gggagcgaga
     7021 cgttttgtta ttatgggaat accacatgtg ggggatactc ccaagtttgt gaaaactact
     7081 gatcgtgacg tgttaaatgc agcggtagac cttcataatg agcgccttgc aaaacggatt
     7141 gacgcatgga aagagcggta tccaacagct gacttcttgt ttgtaaatac agagcaatac
     7201 ctgaaaaaag cgtttgaaga tccggagaaa tatggttttt ataatgtgaa agatgcctgt
     7261 atcgatgtga agttccccat gttcaatgca ttcattcact ctccatttgc tcgtaactac
     7321 gtattgcaat acacacaggt tcttcaatat cgagataagc aatttgctcc aggtgaaaat
     7381 aactatcaca tgtgtagtga accagaagac tatttattct gggatgaaat tcatccaacc
     7441 acgagagcac ataactatct tgcttatgag gtttgtatgg ctttggagga acatggctac
     7501 gaggtaactt gcaaaatgcc agagtccgcc taatacaatt aggtatcatc acttgaacca
     7561 cattaacagt ggttcaggtg atttcatgat gaagttgtct aaattgatcc tcatttaagt
     7621 aaatccagag cggtttttaa gcaattcttg agtctctaaa tgatgacgaa gattaatttg
     7681 ccttagtgat ttgtaaaact ttatgtcata attccccttt tttattgata tacttcattc
     7741 atatgaggga atattattta taaaatttaa ggcaccggtt atttaggaaa ttagctgcat
     7801 tcttatgcgt ttcactcttt atctatatat agtagatgaa ccaagaatga aaaagcttat
     7861 ttctacaaaa acagttgctg aaaagaggag caggttatgt gcgggattat gggagctgtt
     7921 tcagaacggg atattagcaa gattttattg gaaggcttgc gccgattgga atatagaggg
     7981 tacgattctg caggtattgc tgtcatagat agtcaagatc gtttaaagag agtaagaatc
     8041 caggggaaag tgcagaattt ggcggatgct atgcaagaga ccgctatcgc tggaaataca
     8101 ggtatagctc atactcgttg ggcgacgcat ggtaaaccct ccgaacaaaa tgctcatcct
     8161 catttatctc atggagaaat cgcattagtt cacaatggca ttattgaaaa tcatgaacat
     8221 ttgcgtcaac aacttattac ttatggttat caattcacat ctgaaactga tacagaggtt
     8281 gctgcgcatt taattcatta tcattaccag cagcatgaga atttattact tgctgtacaa
     8341 aaagctgctg cagaaatgca aggggctttt gcattaggag tgattcatca gaaaagacca
     8401 gaagaattgg ttgcaattcg taaagggagt cctttggttt tgggatttgg aattggtgag
     8461 aatttcattg catctgatgc cttggcactc agatctttcg cacaatcagt tatttacatg
     8521 gaagagggtg acagtgcttg tgtcacaaca caggatatta aggtctatga ttccaatcga
     8581 atacttgttc aaagagcggt tcatccgttg aacagtgatt ctgagatagt gaataaaggg
     8641 ccatacaggc attttatgct gaaagaaata tttgaacaat cgaaggtaat cacagacacg
     8701 ttagaaagtc gaattaatag cattgatgtg ctaagagcga gttttggtga gaaagcatcc
     8761 catattttcc cggtggtaaa aaatattcac attgtggcat gtggaaccag ttatcatgct
     8821 ggaatgattg ccaaatattg gcttgaatca cttgccggct tacctacaca ggttgaaatt
     8881 gcaagtgaat atcgataccg agatgttgtt gtccccgaca ataccttatt tatcactgta
     8941 tctcagtcag gagaaacagc tgatactctt gctgccctat ttaaggcaaa gcaatcgaat
     9001 tatttggcta gtttagctat atgcaatgtt gcaaccagta ctttagtgag agaggcggat
     9061 tgtgtttttt taactcgggc aggtattgag attggtgtag catctaccaa agcgtttact
     9121 acacagttag cagccttcct gatgttggca gcagcacttt gtaaggacaa tcgtgcgcag
     9181 gaagttttaa gacaattaca agagctgcct gcctgttgtg agcgtgtttt gcagatgaat
     9241 gaagaagtcg aatcattggc ttctttgttt gtaaataaag ttcatgcctt atttttaggt
     9301 cgaggggttc aatatcccgt agctctggaa ggcgcattaa aacttaagga aatatcttat
     9361 atacatgcgg aagcttatcc tgccggtgaa ttaaagcatg gtccattggc tttggttgat
     9421 aaggatatgc ctgtgattgc agtagcccct aatgatgaac ttttagataa attaaaatcc
     9481 aatttacatg aagtgagtgc cagaggtggg cagttatttg tatttgttga tgattcacaa
     9541 aattggaaag cgaatggcgc tcgtttaatc aaagttccat cctgtggtgc ttggcttgcg
     9601 cctattgttt acactattcc tttgcaattg ctggcctatc atgttgctgt agctaaaggg
     9661 actgatgtgg atcaacctag aaaccttgca aaatcggtga ctgtagagtg aacaaaggtc
     9721 aatatttttg gaatgagtta tggtgcgagg gacgtatttc ttttcataaa gaagaagtaa
     9781 atccggattt gatagcctat atctcttcct taaatatacc tgccaaggga agggttctgg
     9841 ttcctttgtg tggaaaaagt gtcgatatgc tttggttagt tcgccaaggg tatcatgtgg
     9901 ttggtatcga actggttgaa aaggctattc ttcagtttgt tcaggagcac caaatcactg
     9961 ttcgggagaa tacgacaggt caggcaaaac aatattttac agataatttg aatttatgga
    10021 ttaccgatat ttttgcttta aacgcggctt taatagaacc agttgatgct atctatgaca
    10081 gagcggcttt ggttgctttg ccaaaaaaat tgcgaccagc gtatgtagat atttgcctca
    10141 agtggcttaa gcctggggga agtattctgt taaagacttt acaatataat caagagaaag
    10201 tgcagggtcc tccgtatagt gtttccccag aagaaatcgc tttatcgtat cagcaatgtg
    10261 ccaaaataga gttgttgaaa tcgcaaaaac gaattcggga gcctaatgat catttattta
    10321 atcttggtat tagtgaagtg aatgattatg tttggcgtat taggaagggg taaaacgtag
    10381 tacagtgtta cgcataagga cattttgtat ggatattagc ttaatttcta tatcgcatta
    10441 tagtatgtga tgagtatata atgagacgat ttttatcatg ttttacgggg atatgaatgg
    10501 cagaagtgat gttggcgtca gccgcaatta ttgcagatga acttaaggtc aaagtatcgc
    10561 aggtagaaac tgctattcgg ctgttagatg aaggagcaac agtacctttt attgcgcgtt
    10621 atcgcaaaga agcaacggga gggcttgacg atgtgcaatt gcggtttctt gcggaaaggc
    10681 taatttattt gcgtgaattg gatgagagac gaacagtcat tttgcaatcc attcgtgaac
    10741 aagaaaaatt aacaccggaa ttggaacaaa gcatattagc tgctgaaact aaaacacgcc
    10801 tggaagactt gtatttaccc tataggccta aacgtcgtac aaaagcacaa attgctattg
    10861 aagcaggatt agagccatta gctcatgctt tatggaaaga tcctgctttg gatcccgaag
    10921 agcaggcttc ccaatttata aatagcgaag cagggattga aaacactcag gccgccctgg
    10981 atggtgcgcg acatatttta atggagcatt ttgctgaaga cgccgagtta attaacgaat
    11041 tacgagagta tttgtggcaa cacggaatag tcaaatcagt aggaaacagt actaaaaagg
    11101 atcaggcgaa taaattttct gattactttg attattcgga agcgattaaa aaaattccat
    11161 ctcatcgggc actggctttg ttccgggggc ggcgtgaagg aattctgcaa gtaagtctgg
    11221 ttttacctga tcaggatgcc aattatggag agaataaggt tgctgcttac tttaatgtca
    11281 ctgacaggca aagaaaagcg gatggctggc ttatagatac ggtcaggatg acttggaaaa
    11341 ttaagttatt taccaagctg gagcttgagt tattaactcg tctgcgcgaa atagcagatg
    11401 aggaagccat taaagtgttt tccaaaaatt tacgtgatct gttgctggct gctcccgcag
    11461 gtcctcaagt gacgataggt cttgacccgg gaattagaac gggtgttaaa gtggttgtcg
    11521 tggatgctac aggtaagttg ttggattact ctgttatttt tccatttgct cctcaaaatg
    11581 aatggcatca atccattgcg gatttggcca agcttgctgc gaaatatcag gttaatttaa
    11641 tcagcatagg aaatggcacc ggttctcggg aaacagagag attggtagct gatttaatta
    11701 aaatgtaccc tgatttgaaa ttaacaaaaa taattgtcag tgaagccggg gcatctgttt
    11761 attccgcatc tgaaattgca gccaatgaat ttcctgattt ggatgtttcc ctaagaggcg
    11821 ctgtatctat tgcacgaaga ttgcaagatc ccttggcaga actggtaaaa attgaagcca
    11881 aatcaatagg tgttggccag tatcaacatg atgtgaacca ggcaaagttg gcgcgttgcc
    11941 tggatggtgt tgtcgaggat tgtgtgaatg ctgttggcgt agacattaat acggcctctg
    12001 cagctttgct atcccatatt tccggattga atgatacttt agctaaaaat attgtgcaat
    12061 ttcgcgatga acatggtgct ttcacaaaca gaaatcaatt aaaaaatatc catcgtatgg
    12121 gagaaaaggc ttttcagcaa gcagccggct ttttgcgcat tatgaatgga gataatgctt
    12181 tagatgcctc ttgtgttcat cctgaagcct atcctttagt ggaaaaaatt atttcagata
    12241 aaaaagttag tattaaggaa ataatcggca atagagaatt attgcagagc attaacgctg
    12301 aacaatatgt ggatgagaat tttggtttgc caacaataag agatgtttta cgtgaattgg
    12361 agaaaccggg ccgagatcca cgaccagaat tcagaaccgc tcaatttcag gaaggtgttg
    12421 aggacattaa agatttggaa caaggaatgg ttctggaagg tgttgtgagt aatgtgacta
    12481 attttggtgc ctttgttgat attggcgttc accaggatgg gttggtacat atttcagcaa
    12541 tgaccaatcg ctttattaca gatcctcgtt ctgttgttaa agccggtgat attgtaaaag
    12601 ttaaagtggt tgaagtggat aaagaacgcc gaaggattgg tttaagtatg aagctgaatg
    12661 atgagcaggc tcctgctgtc ttgcagaaag ctaataaacc acagccggct agaaaacaag
    12721 cggaacaaaa gcagcctgaa tttaagaaga agcaagaaca taagaaaaaa ccagataaga
    12781 taaaaaaatc ggagccagtc aaaaaaaccg tatttaatac agccatggcc gatgctttgg
    12841 ccaaactaaa acgtggtgga gaatagggat gcaaacacct aaaggagagt tgactatcca
    12901 aactttggcc atgcctttaa atactaatgc caatggcgat atatttggcg gatggatagt
    12961 ctcccaaatg gatttggcag caggtgtttt ggcaaagaga atttcccatg ggcgtgtcgc
    13021 taccgttgca attaattcaa tgactttttt aaaacctgtg catgtaggcg atgtagtgag
    13081 ctgccacgtt gaattagtga aagtaggtaa tacctctatg actattcggg ttgaggtatg
    13141 ggcttactca tcgatcaaaa tggaaaaata ccaggttact gaagggatat ttgtttttgt
    13201 tgcgattgat gaaaatggaa agccgaggca ggtacccaaa acacaatgac taataaaact
    13261 caagaattgc agcgagtaat tgctgatatg gctgtattga ttgagcaaga tgcagagcct
    13321 gaccctcaat tgtatgcctt ttttttccaa catccggaaa ttagtcttga attaatacac
    13381 cttattaata atttgagtga agaattagtg catgatgggc ctccaatata ctcagcatgc
    13441 gttttcgctt tagacatttg tattgctcaa ttgcaagcag ctgcagaggc aaataataaa
    13501 aatgccacaa aattattgtc tcaactaatg aatcatttag ctgaggtcat tgatacacat
    13561 aagcatagct taagcttttg gttacccgta ctgaatgcat tttatgaagt gcatgttgag
    13621 ctaactgacg atttaaagga agcatattat ggcttggcaa gccaggatga tgatgcggta
    13681 gaagtggatg agcaatttaa tcatttggac tccataaaag atttgatcca tgaattatct
    13741 gatttaaccc tgtttgacat agcagagagt ttttttgcac aaagctacgc catgccgcct
    13801 gatttttttg cagatttgat tgttgatttg tatagtattg aagaaggtaa agatattgca
    13861 ctactggttt tactccatcc taatatcgag gtacgagaga tagcgctatc tactatcgat
    13921 caattaataa atacaattac tttgacgcca gcatcactgt caagattaca aataatcaaa
    13981 tattggtatc caacccatta ccatggaatg tttgatcgtt ggattaaaac gcaaagaaaa
    14041 aagggagtaa catttgaaac agaggctagt caagcaaaaa taacgattaa agcaactgaa
    14101 gtggatggca gtggttcgca aggagttttt atccacgcaa ggaaaaataa aaaaaacagg
    14161 ttatgcggct tattattaaa gtatgatttg ggcattaaag atgcctggat aactccagag
    14221 atttctgcag cggaagttac agattattat cataaagcgt ttgaagacag cgtgaccttg
    14281 cgtgaagttg atttctctta ttttcaaaca atagtggagc attttttagc cattactgtt
    14341 gaaaaaagtg atatacctaa tttgcatttt ttagaaatac tcgaatttct gggcattcgt
    14401 ttgaaacctg ataagcttga tttggattgc gtatttgaac aattaagtgt tcagattatt
    14461 ccttttactc aagaaaaaat agcagaatct ttaaaacgct cgaaaatgtg gcttaaaaat
    14521 aagcaattta cagaatcatg gtatattgaa aatcccacca ttgataaaat tgtcaatcat
    14581 aacagcagct ttgtggaagg agttaaagtt tgtcgtttgg ctgatgctat agacgcagta
    14641 ttttccgagg aaatggaatt acaccgggat aaatggcagt ttcatttttt atggatagcg
    14701 ttatgggtta aggcaaaaca caaaaaaaat gaaaaggttt ggcaggatag ttttttaata
    14761 gcttatgcta ttcatcaagg atacccattg aaagatattc cagtaatgaa agagatatgt
    14821 tatcaaacag ttgtcaacag catagaaacc atgcaggaaa gaagaacaca cttgagccag
    14881 gaatgaatag aaaaatgttc cggaattatt cttgttaata aactaatatg tagaacagag
    14941 ttaatggccg ccaaatgtgg ttgaaagacc aaccctttct ttttcttgca gggatgggga
    15001 tggatcggta tgctcgcgcc ttacttcagc tatgattttt ctatactcta cggcaggatt
    15061 gatatccttt tgattagaga ctgtttcctg attttcagag gtagaaacct taaccggttc
    15121 aggttgaact tcaactttaa agtc
//
LOCUS       DACTJE010000072        14728 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.072, whole genome shotgun sequence.
ACCESSION   DACTJE010000072 DACTJE010000000
VERSION     DACTJE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14728
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.072"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..2601
                     /gene="sdeB"
                     /locus_tag="I9760_001689"
     CDS             <1..2601
                     /gene="sdeB"
                     /locus_tag="I9760_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdeB"
                     /protein_id="HAT5043458.1"
                     /translation="ALTECGEALDKQNVTEALQALNKLPAEKEIGKAKGIGQELRGQI
                     VGVKQELTGNLESLQRAVTTPVVKDAEKMRVRYETLLTDVTKRVTDFEKIKPANLDGY
                     NKAIADLNNIQQELNLLRNEKIRMHTDKDKAVDFSDIEALEKRLKEAQPNLYPSLVEG
                     TTKGIQELEKIPKSISFDDVKSMTSKINGYLETLEFIRNERVKKHGESTEPLDMSDLD
                     KLKNQLQGVSQHLAQILLNGAKNSLDKIKDPVTLEKEGQYIKRCLDHFAELEKTLDSS
                     VKGMKQKEDFNTYKNSLMEKQEKAYPEMLQLQYKSEALITQLRNLCNIHHDNLAKTRE
                     EQQQLLNKTGGILGGFFSQITTAISQAESKKANEFARFKTELNNDKSDVSQLIQFLVK
                     KKPSELEENLGISKENAEQLHGLLKQLDAKATPIDKLQENTRLIDEISTKMGSKPVSP
                     EHVAATKAREYYQFLLYGATQKIGNFEKIKPIDPESYKKAMSDLNNMQEALQFLRSEK
                     SRIHDGKEKAVEFSDIEALEKRVQNAQPKLLTALLDKTTREISALVKIPRKLTFEDIK
                     SMTTKLNSYLETLELIRNERIKQHGKSPELLDMSDLDSLKGQLQTFNQNLTGMILNAT
                     KDGLDRINDRANFKEGEPYVKACLDLLTELEKTLDSSVKGMKQKEDISACRNSLLDKQ
                     EKANSGMLDLQSKSKDLVTQLRDICKIHHGNLAEARRTRLHSLDNQEGGLLGGLWSVT
                     NKLGVTTDTVGIERMQIKMKEQALARFKTELNNDKYDTNQVIAFLANKKPSELEEGLG
                     ISKENAEELHGLLSKLTSKMTSKIEIEENTQLIDEISTKIGTEPVKLESTHTVDEDER
                     DTYHRESGYF"
     gene            2980..7479
                     /gene="sdeA"
                     /locus_tag="I9760_001690"
     CDS             2980..7479
                     /gene="sdeA"
                     /locus_tag="I9760_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector NAD-dependent ubiquitin ligase
                     SdeA"
                     /protein_id="HAT5043459.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGYGDITSGSIYNGVPTIDTGAL
                     NRQGFMPVLTGVGPHRDSGHWIMLIKGPGNQYYLFDPLGKTSGEGYQNILAAQLPMGS
                     TLSVIPNGSGLNMGLCGYWVASAGLRAHQALNQHNPPTLLNVGQTITNEMRNELDHDG
                     YRKITGWLRAVADEFPEGDPQLDGKALRENTEKDLKIEIPTLVLPGKDTSPKEMSVKP
                     TAPQDKSVPVWNGFSLYTDDTVKAAAQYAYDNYLGKPYTGSVESAPANFGGRMVYRQH
                     HGLSHTLRTMAYAELIVEEARKAKLRGETLGKFKDGRTIADVTPQELKKIMIAQAFFV
                     AGRDDEASDAKNYQKYHEQSRDAFLKYVKDNESTLIPDVFKDQEDVNFYARVIEDKSH
                     DWESTPAHVLINQGHMVDLVRVKQPPESFLQRYFSSMQRWIGSQATEAVFGIQRQFFH
                     ATYEVVAGFDSDNKEPHLVVSGLGRYVIGEDGQPIREAPKKGQKEGDLKVFPQTYKLK
                     ENERLMRVDEFLKLPEIQNTFPGSGKHLQGGMPGMNEMDYWNRLNSLNRARCENDVDF
                     CLKQLQTAHDKAKIEPIKQAFQSSKGKERRQPNVDEIAAARIIQQILANPDCIHDDHV
                     LINGQKLEQQFFRDLLAKCEMAVVGSLLNDTDIGNIDTLMRHEKDTEFHSTNPEAVPV
                     KIGEYWINDQRINNSSGNITQKKHDLIFLMQNDAWYFSRVNAIAQNRDKGSTFKEVLI
                     TTLMTPLTSKALVDTSQAKPPTRLFRGLNLSEEFTKGLIDQANAMIANTTERLFTDHS
                     PEAFKQIKLNDLSKMSGRTNASTTTEIKLVKETWDSNVIFEMLDPDGLLHSKQVGRHG
                     EGTESEFSVYLPEDVALVPVKVTLDGKTQKGENRYVFTFVAVKSPDFTPRHESGYAVE
                     PFLRMQAAKLAEVKSSIEKAQRAPDLETIFNLQNEVEAVQYSHLSTGYKNFLKNTVGP
                     VLENSLSGLMESDTDTLSKALAAFPSDTQWSAFNFEEARQAKRQMDAIKQMVGNKVVL
                     DALTQCQDALEKQNIAGALDALKKIPSEKEMGTIRRELREQIQSARQELESLQRAVVT
                     PVVTDEKKVRERYDALIENTSKKITELETGKLPNLDAVKKGISNLSNLKQEVTVLRNE
                     KIRMHVGTDKVDFSDVEKLEQQIQVIDTKLADAYLLEVTKQISALDNTKPKNQTELKT
                     KIAAFLDRTTDIEMLRNERIKKHGSSKDPLDLSDLDKLSGSLQRINQSLVSDLITTIR
                     VSINQMEAKTFHEQEKEIQQNFELLAKLEKTLDKSKTSEKLREDIPKLNDLLVAKQKA
                     YPQMVQMQLKSEVFVTQLREVCQANHDDLDKTRNARLRELDRLDREAGITRMVGNLIW
                     GLTNKVGLTTDERLDIRTKQQSLARFKNELFNDKIDTDQLISNLARKRPSELQEGLGI
                     STDNAMELHLLLTELAGKTTSPDELEERMKAIDDISTKIGREPEHLKFVMVEEDESNK
                     KTIGF"
     gene            7899..8522
                     /locus_tag="I9760_001691"
     CDS             7899..8522
                     /locus_tag="I9760_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5043460.1"
                     /translation="MSLSSVKKVYNIYSSFYDVLFGSIFHQGRSLCTNKVNKNAGLNA
                     SVLELGVGTGLSLPFYRPDLNITGIDISEKMLEKAEKRIVKKKLTTHIDLKIMDAANL
                     EFPDNHFDFVVAMYVASVVPDIDTFLKEISRVCKPSGEIIFVNHFASEKPILRFFEKQ
                     LSHIDNFVGFNSNFSIHSILNYKEFKLLETQKVNLFGYWKLLHCKIN"
     gene            complement(8551..9672)
                     /locus_tag="I9760_001692"
     CDS             complement(8551..9672)
                     /locus_tag="I9760_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAT5043461.1"
                     /translation="MNALTSWIKAWSQKFFPNQKKNENKEEEADLTQEKKVRPNADFY
                     ENYFSLVAAGTRLKLIEAMFGVNLFSLFENKAWVLEKDIIEKLQLMPIRARKWLHLLS
                     SEHFLIQIKVNGQQAYQLPEEFIELIHSEEFPAMEMFFKTWLVSAEENLTDVLRFGKV
                     KTSVSWPPKTQQEAEWLEDWMTRTSDQPTYCLLQSINFKKVNKFLDVGGGDGTMACTF
                     VKKYPHLKAAVYNLPMSAQMARKNIESRKLNHRVHVIEGDFIEKDAFPVGFDLILFTR
                     VLFDWDDQVCRKLLRMAYQALPKNGLVGICEFYKEDNNDICLASEYRYIFHDDFGVNV
                     MKRASEYQNMLEKIGFTIVQPHKVKGMHFYYCSLILARK"
     gene            complement(9965..11413)
                     /locus_tag="I9760_001693"
     CDS             complement(9965..11413)
                     /locus_tag="I9760_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2160 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043462.1"
                     /translation="MFSANQSLDFAWLSMQMFLINLLKDKIMRYAVTYCAMDHEFSGN
                     PFWHSCILLSQWDDNGKIEVVDNWGFYGVPSTVRDTWLSKLKIKLGLDVDLKGNHGML
                     RHEELRYLDVGYGLHGVTFELTKEKFDLLQLKCQTMLEEQKQAVKEVVESQGIAGKQK
                     YRIYEHEEYSPLIFALEKIKAKQKGHQPRLKPFELNLSFTLWGPALNQSYTCKSLMVD
                     LLKGILTQEQIARITEAGKHPTVPRYSGKLERIYLHSSGPLREHKKSSGAIVHYRDLQ
                     DEGVKLHWTLPPQEIEALSGDTIKLLEISEEYCDEVKQAISKLQKLEWLFIDAKLPEK
                     YKPYQDDLVARIRQYYEAFAQVEPKKAKSTATGWMGFAFSLLSLPRDLDEKCLLQKLN
                     NAKYLINSLYMAIVDHWKIYDDWPSETKEDSTEYNPLEALAAYLCEEDKKKLCKIIGR
                     NYLDPSSEEEFDDIEESEWEQPELSRSVASPM"
     gene            11942..14200
                     /locus_tag="I9760_001694"
     CDS             11942..14200
                     /locus_tag="I9760_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HAT5043463.1"
                     /translation="MFAEIPKIQEQLHNVLEYSQSMTSLWMQSIDKSLEQTNKVTQSL
                     INLNQQQIYPYPNILNDAIEYTVDFMQRSILFWDIMRKRGNQYLKHEQEGQPPVLIFS
                     YNMLMDGRHFERPVNYALVEIIPPEGIQIDPAKRPYVIVDPRAGHGAGISGFKDASQV
                     GLALRAGHPVYVIIFFPVPEPNQTLVDVTAAHEKFLTEVALRHPQSPKPCLIGNCQGG
                     WAILTLMAANPDVAGVAVVNGAPLSYWGGENGKNPMRYIGGIWGGSWIAQMAGDLGNG
                     KFDGANLVMNFEMANPKVTYWSKYYNLFANLDKEEIRFLNFERWWGGFSLLNVNEMRG
                     IIDNLFIGNKLVHGKIPLGQSGNNLDLRNISVPVIIFCSEGDNITPPQQALNWVSDLY
                     SNTLEIKLDGQVIVYLIHKSVGHLGIFVSSEVAKKEHNQIIDLLNYIEHLAPGLYELK
                     LNETKENPKSPPHYLAYIEERSINDIRKGQESNVAIFNLVRMVSEYNAMSYDLFMAPI
                     IRNISNEYTAELIRKLHPLRQNQYLLSDLNPLVHPVSWISPFIRQNRIKISENNPFLT
                     QQLNFSKLINALWDFFSASRDNTVELMFYAVYGYIQLLAPFDPRRDMIVHSPEKDNQE
                     KITQSIIAHISDGGVPEAILRILLLLVKIQGYIIGANLPDVIQKLRECNALKHLDKNG
                     FKQIVHSQTIMIEHDPELAFNTIPHLLKSQEERSMVIQFIEDILKSLKVSPSEEYKKK
                     FQRIKELLQNQV"
     gene            14377..14728
                     /locus_tag="I9760_001695"
                     /pseudo
     CDS             14377..14728
                     /locus_tag="I9760_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947023.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5078 a         2837 c         2690 g         4123 t
ORIGIN      
        1 gctctcacgg aatgtggtga agcactggat aaacaaaatg tcacagaagc attacaagcg
       61 cttaataaac tgcctgcaga aaaggaaata ggtaaagcca aggggatagg acaagagtta
      121 agagggcaaa ttgtaggtgt taagcaagag ctgactggaa atcttgaatc cttgcaacgc
      181 gcagtcacta cccctgttgt aaaagatgct gagaaaatga gagtacggta tgaaacgctt
      241 ctcacagacg tgaccaaacg agtcaccgac tttgaaaaaa tcaaaccggc caatcttgac
      301 ggttataata aggccattgc cgatttaaat aacatacaac aagaattaaa ccttttgcgc
      361 aatgaaaaaa ttcgcatgca tactgacaaa gataaagcag tggacttttc tgacatagag
      421 gccttggaaa agagattaaa agaagcacag cccaatttat atccctcgct tgtggaagga
      481 acaacaaaag gtattcaaga acttgaaaaa atacccaagt caatcagttt tgacgatgtt
      541 aaatcaatga catccaagat taatggctac ctggaaacct tggaattcat tcgtaatgaa
      601 agagttaaaa agcatggtga atccactgag ccactggata tgtctgatct ggataaatta
      661 aaaaatcaac tacaaggcgt gagtcagcat ttagcccaaa ttttattaaa tggagctaaa
      721 aactcactcg ataaaatcaa ggatccggtt acactcgaaa aagaaggaca atatataaaa
      781 cgctgccttg accattttgc tgaacttgaa aaaactctgg atagttcggt aaaaggaatg
      841 aaacaaaaag aagactttaa cacctataaa aactctctaa tggagaagca ggaaaaagcc
      901 tatccagaaa tgttgcaatt gcaatacaag agtgaagcat tgattacaca attacgtaat
      961 ctatgcaata ttcatcatga taatttggcc aaaacgagag aggaacaaca acagctactg
     1021 aacaagaccg gaggaatatt aggcggtttc tttagccaga taacgactgc tataagtcaa
     1081 gccgagtcaa agaaagcaaa tgaatttgca agatttaaaa ctgaattaaa taatgacaaa
     1141 agtgatgtga gtcaacttat tcaatttctg gtaaagaaaa aaccatccga attggaagaa
     1201 aatttgggca tatcgaaaga aaatgctgag caattacatg gactgttaaa gcaactagac
     1261 gcaaaagcta ctccaataga taaattgcag gaaaacacaa gattaattga tgaaatatca
     1321 accaaaatgg gtagtaaacc cgtctcgcca gaacatgttg ccgcaacaaa agcgagagaa
     1381 tactatcaat ttcttctata tggagcgaca caaaaaatcg gtaactttga aaagatcaaa
     1441 cctatcgatc ccgaaagcta taaaaaggct atgtcagact taaataacat gcaagaagca
     1501 ttacaattcc tacgtagtga aaaatcccga atacatgatg gcaaagaaaa agcagtggaa
     1561 ttctctgaca tagaagcctt ggaaaaacga gtacaaaatg ctcaacctaa attactgaca
     1621 gcactcctgg ataaaacaac cagagaaatc tcagcattag taaaaatacc caggaaattg
     1681 acctttgaag atataaaatc aatgacaacc aagcttaaca gttacctgga aaccctggag
     1741 ttgattcgta atgaaagaat caaacaacac ggtaaatccc ctgaacttct ggatatgtcc
     1801 gatttggata gtttaaaagg tcaattgcaa acctttaatc aaaatttgac tggcatgata
     1861 ctaaatgcta ctaaagatgg tctagataga atcaatgaca gggctaactt taaggaagga
     1921 gaaccttacg taaaagcatg tcttgatctt ttaacagaac ttgaaaaaac actggatagt
     1981 tcggtaaaag gaatgaaaca aaaagaagac attagcgcct gtagaaattc tctccttgat
     2041 aaacaggaaa aagccaattc cggaatgctg gatttgcaat ccaagagtaa agatctggtt
     2101 acccaattac gtgatatctg taaaattcat cacggtaatt tagctgaagc aagaaggacc
     2161 aggcttcact cacttgataa tcaggaaggc ggattattag ggggattatg gtctgttact
     2221 aacaaattag gtgttaccac agacacagta ggtatcgaga ggatgcaaat aaaaatgaag
     2281 gagcaagcac tggcaagatt taaaacagaa ttgaataatg acaaatacga tacgaatcaa
     2341 gtcattgcct ttttagcaaa taaaaaacca tcagagctgg aagaaggttt aggcatatcg
     2401 aaagaaaatg ccgaagaatt acatggactg ttaagtaaac tcactagtaa aatgacttcg
     2461 aaaatcgaaa ttgaagaaaa tacacagtta attgatgaaa tttcaactaa aataggtacc
     2521 gaacccgtaa aactcgagtc gactcacaca gtagatgaag atgagcgtga tacctatcac
     2581 cgagaaagtg gttactttta agtacttgtt cgatggtgaa atgattcagt gaatgatcgt
     2641 aagcgctagc agatgagaaa tgaaagacat tatatgaatt agtttctcat tctgttaatc
     2701 gctttaaaac atgtcacttc taatgaagaa acactataaa gatagggtca acctcttttc
     2761 cgctcaaatt caccttattt tactgccctg ctctttatac aagaggaaca cctcccaatt
     2821 ttttttcaaa tatcaaattc aaaaaaaaat gaatattttt tttacattta atactttctt
     2881 aatactacaa ttctataata aacatacaat ttgaaatatt tatattcatc taatgtgcac
     2941 atttgcacgt tttagaatat cagtttggga gaagccataa tgcctaagta tgtcgaaggg
     3001 gtagaactaa ctcaagaagg tatgcatgct atttttgcac gcatggggta tggagatatt
     3061 accagcggta gcatatacaa cggagtgcca actattgaca caggtgcgct aaacaggcaa
     3121 ggttttatgc ccgtgttaac cggggtagga ccacacagag actccggcca ctggattatg
     3181 ttaatcaaag gtcctggcaa ccaatactac ctctttgacc cattaggcaa aacatcaggt
     3241 gaaggctatc aaaatatttt agcagcccaa ttaccaatgg ggtctaccct ttctgttatt
     3301 cccaatggtt ccggtttgaa tatgggatta tgcggctact gggtcgcctc tgctggttta
     3361 agagcacacc aggcattaaa tcaacataat ccccctactt tgttaaatgt gggacaaacc
     3421 attaccaatg aaatgcgtaa cgaactcgat catgacggtt atcgaaaaat taccggatgg
     3481 ttacgtgctg ttgctgatga attccctgaa ggtgacccac aattagatgg gaaagcatta
     3541 agagaaaaca ctgaaaaaga tttaaaaata gaaattccta cactggttct tcctggaaaa
     3601 gatacctccc ctaaagaaat gtctgttaaa cccactgcac cacaagataa atctgtgcct
     3661 gtttggaatg ggttttcatt atatactgat gataccgtta aagccgcggc acaatacgcc
     3721 tatgacaact atctgggcaa accgtatacc ggatcagttg aatcagcacc tgcgaatttt
     3781 ggcggtcgaa tggtgtacag acaacatcat ggactttccc atactttacg gaccatggct
     3841 tatgcggaat tgattgttga agaagcacgt aaagctaaat tacggggtga aacattaggg
     3901 aaattcaaag acggacgtac tattgctgac gttacccctc aagaactaaa aaaaatcatg
     3961 attgcccagg cattttttgt agctggacgt gatgatgaag catccgatgc caagaactac
     4021 caaaaatatc atgaacaaag ccgggatgct ttcttaaaat atgtgaaaga caatgaatcc
     4081 actttgattc ctgatgtttt caaagatcag gaagatgtca atttttatgc ccgagtgatt
     4141 gaagataaaa gtcatgattg ggaatcaacc ccagcccatg tgctaattaa ccagggccat
     4201 atggtcgatt tggtacgggt caagcaaccc cctgaatcat tcttacaacg ctatttcagt
     4261 tccatgcaac gctggatagg ttcacaggca acggaagctg tttttggaat acaaagacag
     4321 tttttccatg ctacctatga agtagtcgca ggttttgact ccgataacaa agaacctcat
     4381 ttggttgttt ctggtttagg tcgttatgtc atcggtgaag atggtcaacc tatccgagaa
     4441 gctccgaaaa aaggacagaa agaaggagat ttaaaagttt tccctcaaac ttataaatta
     4501 aaagaaaatg agcgtcttat gcgcgtcgat gaatttttaa aacttcctga aatacaaaat
     4561 accttccctg gttctggcaa acatttacaa ggtggaatgc caggtatgaa tgaaatggat
     4621 tattggaatc gactgaactc tttaaacaga gcacgatgcg aaaacgatgt tgatttttgt
     4681 ttgaaacaat tacaaacagc ccatgacaag gcgaaaattg agcctattaa acaagctttt
     4741 caatccagta aagggaaaga aagaagacaa cctaatgtcg atgaaattgc tgctgcccgc
     4801 atcattcagc aaatcttggc caatcctgat tgcatccatg atgatcatgt gctcatcaat
     4861 ggccagaaac tggaacaaca attcttccgt gacttgttag cgaaatgcga gatggctgtt
     4921 gtaggctcac tcctcaatga caccgacata ggaaacattg atactttaat gcgacatgaa
     4981 aaagacactg agtttcattc taccaatcct gaagcagttc cggtgaaaat tggagaatat
     5041 tggattaatg accaaagaat taataatagt agtggcaaca tcacccaaaa gaaacacgat
     5101 ctaattttcc tcatgcaaaa tgatgcgtgg tattttagcc gagtcaatgc catcgcccaa
     5161 aaccgggata aaggctctac tttcaaggag gtgttgatta ctactttaat gacgcctttg
     5221 actagtaaag cactagtgga tacctcgcaa gccaagcccc ctacaagact atttcgtgga
     5281 ttaaacctgt cagaagagtt taccaaggga ttaattgatc aagcgaatgc aatgattgcc
     5341 aatacgacag agcgtctttt caccgatcat tcaccagaag ccttcaaaca aatcaagtta
     5401 aatgatttaa gtaaaatgtc cggcagaacc aatgccagta caaccacgga gattaagctt
     5461 gtaaaagaaa cctgggactc caatgtcatc tttgaaatgc ttgacccgga tggtttgctg
     5521 cattccaagc aagtaggacg ccatggcgaa ggcaccgaaa gtgaattctc cgtttatttg
     5581 ccggaggatg ttgccctggt cccagtcaag gtcacccttg atggtaaaac gcaaaaaggg
     5641 gaaaatcgat atgtctttac ctttgttgcc gtaaaaagcc ctgactttac accacgtcat
     5701 gaaagcggct atgcggttga accgttttta agaatgcaag ccgcaaagtt agccgaagtc
     5761 aaaagctcta ttgaaaaagc ccagagagca cctgaccttg agacaatttt caatttacaa
     5821 aacgaagtag aagcagtgca atactcacac ttatcgacgg gttacaaaaa ttttctaaaa
     5881 aacaccgttg gtcctgtttt agaaaatagt cttagtggac tcatggagag tgacacggac
     5941 actttaagta aagcattagc agcattccca tccgatacac aatggtcagc cttcaatttt
     6001 gaagaagcaa ggcaagccaa aagacagatg gatgccatta agcaaatggt tggaaataaa
     6061 gtggtgcttg acgccttgac tcaatgccag gacgcgctgg aaaagcaaaa tatcgctggc
     6121 gcactggatg ctctcaaaaa aatcccgtcc gaaaaagaga tgggtacaat tagaagagag
     6181 ctaagagaac aaatccaaag cgccaggcaa gaactggaat cattgcagcg tgctgttgtt
     6241 acccctgtag taaccgatga aaagaaagta agagaacgct atgatgccct gatagaaaac
     6301 acatcgaaga aaattactga acttgaaaca gggaaattac ccaatcttga tgctgtcaag
     6361 aaaggcatat ctaacttgag caacctgaaa caagaagtaa ccgtattacg taatgaaaaa
     6421 atacggatgc acgttggaac tgataaagtt gatttttcag atgttgaaaa actggaacaa
     6481 caaatacagg ttatcgatac caaattagcc gatgcttatt tacttgaagt aaccaaacaa
     6541 atttcagcgc ttgacaatac aaaacccaaa aatcagactg aattgaaaac aaaaatagca
     6601 gcatttcttg acagaaccac tgatattgaa atgttgcgta atgagcgaat caaaaaacac
     6661 ggttcatcga aagatccact ggatctgtct gatttggata aattaagcgg cagcttacag
     6721 cgcattaatc aatccctggt ttcagatttg ataacaacca tccgtgtgtc catcaatcaa
     6781 atggaagcga aaacttttca cgaacaggaa aaggaaatac aacaaaattt cgagttactc
     6841 gcaaaactcg aaaaaacatt ggataagtca aagacatcag aaaaactaag ggaggatatt
     6901 cccaaactta atgatttgct ggttgcaaaa caaaaagcct accctcaaat ggtacaaatg
     6961 caactcaaga gcgaagtctt cgttacccaa ttacgtgagg tatgtcaagc taaccatgac
     7021 gatctggata aaacaagaaa tgcaagatta cgagaactgg atcggctgga cagagaagct
     7081 ggaataacaa gaatggtggg taatctaatc tggggtctta ccaacaaagt aggtttaacc
     7141 acagacgaac gacttgacat caggactaaa cagcaatcgc ttgcacgatt caaaaatgaa
     7201 ttatttaatg acaagattga tacggatcag ctaatctcta atttggcaag gaaaagacct
     7261 tctgaactgc aagaaggatt gggaatatct acagacaatg cgatggagtt acatttactg
     7321 ttaactgagt tagcgggtaa aacaacttct ccagatgaac tggaagaaag aatgaaagca
     7381 atcgatgaca tttccaccaa gataggtaga gagcctgagc atttgaaatt tgtcatggtt
     7441 gaagaagatg aatccaataa aaaaactata ggattttaat tgaccttaac ccaaatcgca
     7501 tgaagttctg aagtcgggtc ttattgaccc gactttttgt tatggattta aaataaaaga
     7561 gaatatatcc atctactggc aacgaaattt cgaccagttt gcataccatt taatttagac
     7621 cttgattctt ctccctggta gctacatcgc ttataaaaga gtcactttga ctttgtaaat
     7681 tcaaaatttt tacatgagat cattgactta tttaagacat atcttcatcc tttggatcat
     7741 ttcgtattgt tggcgcagat attactaaag ataactttga gatgaaatta cctcagtttc
     7801 aacaggagtg aactttttca ttacaatata cgaatttata atccctaaaa gcaaatggtt
     7861 ctatttttcg ttttgtcttt ctaaatggag tattggttat gtcgctctct tcagtaaaaa
     7921 aagtttataa tatttattct tctttttatg atgtcctttt tggatctatc tttcatcaag
     7981 gccgctctct atgtactaat aaggttaata aaaatgctgg cctgaatgct tctgttttag
     8041 agcttggggt tggcacaggg ttatctttac ccttttaccg tccagatttg aacataactg
     8101 gaatcgatat ttcagaaaaa atgttagaaa aggctgaaaa acgtatcgtc aagaaaaaat
     8161 taacaaccca tattgattta aaaattatgg atgccgctaa tttggagttt ccagataacc
     8221 attttgattt tgttgttgcc atgtatgttg cgtctgttgt ccctgatatt gatacttttc
     8281 taaaagaaat ctcacgtgtt tgtaaaccat caggcgaaat aatatttgtg aatcattttg
     8341 cttctgaaaa acccatcctg cgcttctttg aaaaacaact gtcacatatt gataattttg
     8401 taggatttaa ctcgaatttt tctatccatt ccattttaaa ttataaagaa tttaaattgc
     8461 tggaaactca aaaagtcaat ctttttggct attggaaatt attacactgt aaaataaatt
     8521 gaagctttac aaaaataaag ataagggttt ttactttcga gccaatatca aagaacaata
     8581 ataaaaatgc atccccttta ctttatgcgg ttgaacaata gtgaaaccaa ttttttctaa
     8641 catgttctga tattctgagg cccttttcat cacattcaca ccaaaatcat cgtgaaaaat
     8701 gtaccgatat tccgaggcaa ggcaaatgtc gttattatcc tctttataaa attcacaaat
     8761 accgactaat ccattctttg gtaaggcctg ataagccatt cttaataatt ttctacacac
     8821 ctgatcatcc caatcaaaca gaactcttgt aaataaaata agatcaaatc ctaccggaaa
     8881 agcatccttc tcaataaaat ccccctcaat cacatgcact ctatgattta gtttcctcga
     8941 ttcgatattt tttcttgcca tctgcgcaga cattggcaaa ttgtagactg ctgcttttaa
     9001 atgcggatat tttttaacaa aagtacacgc catagttcca tcaccaccac ccacatccaa
     9061 aaatttattg acctttttaa aattgataga ttgtaataaa caataggtag gctgatcaga
     9121 tgttctagtc atccaatcct caagccactc tgcttcttgc tgagttttcg gaggccagga
     9181 aacacttgtc ttgacttttc caaagcgcaa tacatcggtc agattttctt ctgcactaac
     9241 aagccaggtc ttaaaaaaca tttccatggc agggaattct tcactgtgta ttaactcaat
     9301 gaattcctcg ggtaattgat aagcttgctg cccattaacc ttaatttgta ttaaaaaatg
     9361 ctctgaacta agaagatgca gccacttcct ggcccttatt ggcattaatt gcaatttttc
     9421 tataatatcc ttttctaaaa cccatgcctt attttcaaat aaagaaaata aattgacacc
     9481 aaacatggct tcaatcagtt taagtctggt accagcggca acaagagaaa aataattttc
     9541 ataaaaatcg gcattaggcc tcaccttttt ttcttgggtt aaatcagcct cttcctcttt
     9601 attctcattt tttttctgat tggggaaaaa tttctgtgac catgccttta tccacgatgt
     9661 taaagcgttc atcccacaat attcgtttaa gagtgttatt aatatgagaa tagaacagga
     9721 ctgacaaaaa aacccaaggc caaggcaaaa aatgttttta atgagaaaat ttagataaaa
     9781 taaatgactg aactaaaact ctttttaaca aagagattgg ggggcgtaag ttctctagaa
     9841 gtgaaaaacc cgttcagcca gttccctcaa atcacacaat ggtaacaata agtgtacaaa
     9901 tgcagacatc atccagtgta ataatccgca tttgctattt aaaaaaatga cttgatgtag
     9961 gctgttacat aggacttgca acactcctgc tcaactctgg ctgttcccat tcgctctctt
    10021 caatatcatc aaactcctct tctgacgaag gatccagata atttcgtcca ataatcttac
    10081 aaagtttctt cttatcctct tcacataaat aagcagccaa agcttcaaga ggattatatt
    10141 cagtagagtc ttctttagtt tcagatggcc aatcatcata aattttccaa tgatcaacaa
    10201 tagccatgta tagactgtta atcagatatt tagcattatt taatttctgc aaaagacact
    10261 tttcatcaag atctcttggc aaagagagca atgaaaaggc gaatcccatc cagcctgtgg
    10321 ctgttgactt agccttttta ggttcaacct gagcaaatgc ttcataatat tgccgaatac
    10381 gagcaaccaa atcgtcttga taaggcttat atttctctgg taatttggca tcaataaata
    10441 accactccaa tttttgcagt ttgcttatcg cttgttttac ctcatcacaa tactcctcgc
    10501 ttatttccag taatttgatg gtatctccgg ataacgcttc aatttcttgt ggaggcaatg
    10561 tccaatgtaa tttgactccc tcgtcctgca aatcacgata atgaacaatc gcaccggaag
    10621 attttttatg ttcacgtaat ggtccgctgc tatgcaaata aattctttcc aatttaccac
    10681 tgtatcttgg tacggtaggg tgttttcctg cttcagtaat tcgggcaatt tgctcttgag
    10741 tcagtatgcc ttttaataaa tcaaccatca gtgatttaca agtataggat tgatttaaag
    10801 ccggccccca cagggtaaat gacaaattta actcaaatgg tttaagcctt ggctgatggc
    10861 ctttttgttt tgccttgatt ttttccaaag caaaaattaa cggggaatac tcttcatgct
    10921 cataaatacg gtatttttgt ttaccagcaa tcccctgtga ttcaacaact tccttaactg
    10981 cttgcttctg ttcttccaac attgtttggc atttcaactg cagtaaatca aatttttcct
    11041 tggttaactc aaaagtaact ccatgtaaac catagccaac atccaaataa cgcagttctt
    11101 cgtgtcgtaa cataccatga ttgcccttca aatcaacatc cagacccaac ttgattttca
    11161 gtttacttaa ccacgtatca cgaacagtag atggcacccc ataaaaaccc caattatcga
    11221 ctacctcaat ctttccattg tcatcccatt gagataataa aatgcaacta tgccaaaaag
    11281 gattaccact aaactcatga tccatcgcac aataggtaac tgcatatctc attattttat
    11341 ccttaagaag attgattaaa aacatctgca tggataacca ggcaaaatct agagattgat
    11401 tagctgagaa cagtgaactg cttaaacctg actcacttaa tacaatccta tgaaactatt
    11461 acagatcccc atattgccgt caacggctaa acagataatg agataacccc tggaaatttc
    11521 ccaataaaag caaacccaac tgcctaacat ataaattagt agaaataaat ttttaacctt
    11581 tcaaactaaa aacaaaattg atacaaatcc aaagcgtaac aatagaattt cagtgaacac
    11641 ggtgcattta ctgttttgat aataactgtc tataatgatt taaaagtgat taacaaatgg
    11701 gtttaaatgc tcgatgcaca atgcctacta tttgccccag ccagcaaacc ggaacgtgtt
    11761 aaaaggctaa aaaaacgcga atggttttat tatcgtttga agaagatatt tctctaaaga
    11821 gaaaaccgcc ttacgaaaat atgaaaaaag aagagaatga tagcaacata cactgatagt
    11881 caaaatttgt taatgatata gagttaaaga accccgaatt gagaggccac aggagaaaaa
    11941 gatgtttgcc gaaataccca aaattcaaga gcaactccat aatgttcttg aatattccca
    12001 atccatgact tctctttgga tgcaatcaat tgataaaagc cttgaacaaa caaacaaggt
    12061 gacgcaatct ttaatcaact taaatcagca acaaatttat ccttatccca acatattaaa
    12121 tgatgccatt gaatataccg tagacttcat gcagagaagc attctgtttt gggacatcat
    12181 gcgcaaacgg ggcaatcaat atctcaagca tgaacaagaa ggacaacctc ctgttctcat
    12241 tttttcttat aatatgctca tggatggcag acattttgag agacctgtaa attatgcact
    12301 ggtggaaatc attccccctg aaggcatcca gattgatcct gcaaaaagac cttatgtgat
    12361 tgttgatccg cgggctggtc atggagcagg aatcagcggt tttaaagacg cgtcacaagt
    12421 aggacttgcc ttaagggcag gacacccagt ttacgttatt atctttttcc ctgttcctga
    12481 acccaatcaa acgctggtgg atgtcaccgc agcccacgaa aaatttttaa ctgaagtagc
    12541 cttgcgtcac cctcaaagtc ccaaaccctg cttaattggt aattgtcagg gaggatgggc
    12601 tattttaacc cttatggccg ctaacccaga tgtagcaggg gtcgctgtag tcaatggcgc
    12661 tcctctttcc tattggggtg gggaaaatgg aaaaaatccc atgcgctaca tcggaggaat
    12721 ttggggaggg agctggatcg cacagatggc tggtgatttg ggtaacggca aatttgacgg
    12781 ggctaatttg gtaatgaact tcgaaatggc taatcctaaa gtcacttact ggagcaaata
    12841 ttacaatctc tttgccaatc ttgataaaga agaaatccga tttttaaatt ttgagcgatg
    12901 gtggggaggt ttttctcttt tgaatgtgaa cgaaatgaga ggaataattg ataatttgtt
    12961 tattggtaac aaactggtcc atggcaaaat acccttgggg caatcgggta ataacctgga
    13021 tttaagaaac attagcgtgc ctgttattat tttctgctcg gaaggcgaca acattacacc
    13081 accccaacaa gccttaaact gggtttctga tctttattca aatactctgg aaatcaaatt
    13141 ggatggacaa gttattgttt atcttatcca taagagtgtt ggtcatttag gtatttttgt
    13201 ctcaagtgag gtggctaaaa aagaacataa ccagattatt gatttattaa attacataga
    13261 acatttggct ccaggtttat atgaactaaa actgaatgaa acaaaagaaa atcccaagtc
    13321 acccccacat tacctcgctt atattgaaga gcgctctatt aacgacatca ggaaaggaca
    13381 ggagagtaat gtagcaatct tcaaccttgt tcgtatggta tctgaataca atgccatgag
    13441 ctatgacttg tttatggctc cgataatccg taatataagt aacgaataca ctgcagaatt
    13501 gattcgaaaa ttacacccac taaggcagaa tcagtattta ttgagtgatc tgaatccatt
    13561 agtgcaccct gtatcatgga tatcaccttt tattcgtcaa aatcgcatta aaatttctga
    13621 aaataatccc tttttaactc agcaattaaa tttttcaaag cttataaacg cattatggga
    13681 tttcttcagc gcgtctcgtg ataatactgt ggaactaatg ttttatgctg tctatggcta
    13741 cattcaatta ttagctccat ttgatcccag aagagatatg attgttcatt cccctgaaaa
    13801 agataatcag gaaaaaataa ctcaatcaat tattgcccat attagtgatg gaggtgtacc
    13861 agaggctatt ttgcgtatat tactattatt agtgaaaatt caaggctata tcattggcgc
    13921 taacttacct gatgtcattc aaaaactacg tgaatgcaat gctcttaaac atttagataa
    13981 aaatggattc aaacaaattg tgcactcaca aacaataatg atagagcatg atcctgaact
    14041 tgcatttaat acaatacctc atttactgaa atcacaagaa gaacgctcaa tggtcattca
    14101 attcattgaa gatatattaa aatctttaaa agtatcccct tccgaagaat ataaaaagaa
    14161 atttcaaaga ataaaagaac tgttacaaaa ccaggtttga tttccgaatt gggccatact
    14221 gctctcaatc aagcctaaac ctatagatat cctattggaa aatcaatcat tcatctttca
    14281 gacaaatgaa atcccccacc tcaattgttt attttattga cattctgaat gcgttgctat
    14341 aaaagcaatt tttttctaat tggtggtaga cacaatatgt ctaaagaaat taatatcatt
    14401 tgggtaggat cacaacgcct tccctaaaaa tatctacagc atttaaaaaa atggcatagt
    14461 ttaaacccag actatcaaat caatttcatc actgatgaac attcttcttc tattcagctt
    14521 cccggaataa ataccattca ctggaaacaa tgttttgacc tgacggatgt tcgacaagag
    14581 tttttgctga gagaagcgga gcgtgctctc agggaaaaag cctatgctac tttatcgaat
    14641 attgttcgca aggttaaaat ttttaatgat gacgggtatt attttgatac tgatgtcgtt
    14701 cctatagaga aattgcctgc tattactg
//
LOCUS       DACTJE010000073        14698 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.073, whole genome shotgun sequence.
ACCESSION   DACTJE010000073 DACTJE010000000
VERSION     DACTJE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14698
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.073"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            202..1896
                     /gene="sidK"
                     /locus_tag="I9760_001696"
     CDS             202..1896
                     /gene="sidK"
                     /locus_tag="I9760_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector v-ATPase inhibitor SidK"
                     /protein_id="HAT5043464.1"
                     /translation="MGGLTSEQYHSQVVGKIGYIARCMQTIDPENNLKKIREDYQDVL
                     IWAEKNYRFEEILEASKSGKCPNDLDALSRRSLILQELLRLVSSISPFKMKLDLIESQ
                     YEKMKQHVNLWKSDYHVKLNQLNQLTDYLKNAAPTPKNHFLRAMTSALQMQIAQTGIT
                     EDNEGINQLFKLGLHLLAMANEKINEQYDLFKGYVKDQPEESPFEGILPAEDQKILVK
                     AMIDYAMPKLSSKVLQDKLSALSSSDVLTKTLLDSIDRTVEENEKLNALSKVKLGKFG
                     LDIREVEEIYSQALKISPQDALQYTAQQCDAQLLSMAFPDSQNYIVESISDKKAKAIA
                     ELIHSKEFIYQIIKTEVFKQVDPNEKIQLQAATELYQLLGRIMDKQIHLFAKMNLEQI
                     NEYIQTKTKAILDKIPERVELLTFMGFEIPTFKGIETLMTDVSHSQDNETLAIAQEFY
                     ANIKNAKKQLLGDKLIEDITPQDVEKFFNQCSQYGSEAAEKLADNRPALTKIADILTA
                     IARWAISLIGFNTPPQFLAPTRTCVDQVSDEITKIKLKLEDTLGSLQKVQEENLSL"
     gene            2259..2852
                     /locus_tag="I9760_001697"
     CDS             2259..2852
                     /locus_tag="I9760_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0967 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043465.1"
                     /translation="MLPFKYRYMSGADIMTGSSSEQLEEEYLNARREYCMAMMAVHDK
                     ARAFLSETPIDESMKQAIIKSSGVKIRPNETQDEFDERFEDEMGLTIDDYVDFALDVL
                     EKKLEPAKQSIVAAANKELGSLTSGQSGSAWEDFQHTIHEGAEYKTIEVAKCLPESER
                     CIQAFNQAKLAFRDAATNLIHAEKLDEQEEPNHGPKF"
     gene            complement(2998..4863)
                     /locus_tag="I9760_001698"
     CDS             complement(2998..4863)
                     /locus_tag="I9760_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAT5043466.1"
                     /translation="MQKIIILTKKIIAKLPVILFDVAAIPVAWYAAYWLRYNMQPYPG
                     SLTSTHSFIALALLSIVQISCYYYFKIYRGLWRFSSLNDVIRILKATITAMVLVIPVF
                     YLTSILQHLPRSVFPLYCIILATILCGGRLVIRLHWDKPDRGNRELETKRVLVVGAGQ
                     AGEGLVRDLKRSSYYRPIGFVDDNKSKRGLEVHGVRVLGTTNQITELVNQYDVDLIFI
                     AIPSAKSATMRRIVTLCEQSHVPFSTLPSISALAAGRVEVNALRPVNIEDLLGRDQVT
                     LEWEKIARSIAGRRILITGGGGSIGSELCRQVMALEPASIAIVENSEFNLYRIEQELL
                     KSFPGIPVELSLISVTDEIAINHLFRRFQPEIVFHAAAYKHVPMLQHQVRVAVFNNVI
                     GTQVVAKASVTFGAEKFILISTDKAVNPTNIMGTTKRVAEIYCQNLNARVKTQFITVR
                     FGNVLGSAGSVVPLFQKQLQEGGPLTVTHPDMQRYFMTIPEACQLILQAMVNGEGGEI
                     FVLDMGEPVKISYLAEQMIRLAGKEPGRDIMIEYTGLRPGEKMYEELFHETEQLTSTK
                     HEKLFKARFRELDWNDLTQTMRMLQAACIEHQDEELLVLLKSLVPEFQLEPMVEA"
     gene            complement(4930..6477)
                     /locus_tag="I9760_001699"
     CDS             complement(4930..6477)
                     /locus_tag="I9760_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein LphB"
                     /protein_id="HAT5043467.1"
                     /translation="MVLRYPDLYGIELMSAKWRWYDSLFIVLFAYLLVLQIQAIWPFT
                     IDDMYISLRYAKNWAAGNGLLWNLNAEPVEGYSNFSFVALGAISIILHINPVLTLKIA
                     GFAGLLATCLFIYLVSRFWFTKRESFIPCIWLLLYKGQVIWSVSGLETTVYQALISGS
                     VYFAFRGLGYHFYPAPRGEFKTSGFVLSGLYLSFAGMTRPETPALMLLFFLLIGWDWN
                     KLSGKKPWRGAVSFAVTIGLVFLPYFLWRWSYYGLLFPNSVYCKGFNGISYSLDLNYL
                     KLIWPFAILALPACFYAQDRRHYFLWSPGIIYLLMLVNADPVVAFDNRLFLPAFALML
                     PLTLNGISYLLLSYFKKQDNYFRAALYVSSFFLALLFIPKMSLDAYRYFSQNPVHGEQ
                     LRKSVVQWLNTHAKNQDRVVLADSGLIPFLSGLTFIDSYCLNNKEMAQFPAVKRYELF
                     CQQILEEKPQIIILTALIDKGKIIYTPGDSCLKPFLDIHKEYKLVGVFSFGSAESQYR
                     YELFANF"
     gene            complement(6438..7322)
                     /locus_tag="I9760_001700"
     CDS             complement(6438..7322)
                     /locus_tag="I9760_001700"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HAT5043468.1"
                     /translation="MMELANKIIRVSYMDKLYQYLLLLRLSHWSKAIFVMLGFIYTPV
                     PGYLFPALMASLSFCLIASAVYIYNDIQDRTEDSLHPFKCNRPIASEQVTLFDAIIVM
                     LLLMVAGIALGWLISKKLVVILIIYLIINLAYNHVLKLIPVFDVACIALGFMLRVLAG
                     TIGIDLAISAWLTVAATLLSLFIALNKRQLEMQLGLKNSTRKVLRRYNPAFLQRLIVI
                     TGIACFITYLFYTVYARAESFFFILTLPFAAIALWRFAWLATQDVENDDPVNIFLSDR
                     LSRINLWCFAILTFMALN"
     gene            7652..8893
                     /locus_tag="I9760_001701"
     CDS             7652..8893
                     /locus_tag="I9760_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0963 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043469.1"
                     /translation="MRYILPAGALTSLNFLRDDYQSERKVDLDQPPSLIGVPTLFGGT
                     DVIARDKQTKFIKKMIVVLRANLAKEEEITTPEQWEANLTASRVVIAACLYVQSQISS
                     PKNNSALYRLINKDLGITDTNYLDDEDKEICFLAANRVVNSSLSAFSDANAALRKANM
                     KPFTEQEWSDFSKFVSSVSVKKVSTNPYSNYPVTSITQPLFGAAFSYTGATIGYLGGD
                     VISQSSKAMSTKLQITTMVGSSLLMLGTTGPMGVALFSQVIASKLVTAFFSISLAHVL
                     GTTMGILGQGVGMGIGLPLDYAYRLIWKASATIMNYYSNYPNTSQMTGIRIADGKSIM
                     NGIVIEYTPSSDLKTEEGQQTVEITEEGKLLINGKEVTIPDGGVQLPLELINELKAQL
                     KLHEEKVSEEDSSSSTLLATV"
     gene            complement(8979..12425)
                     /gene="dnaE"
                     /locus_tag="I9760_001702"
     CDS             complement(8979..12425)
                     /gene="dnaE"
                     /locus_tag="I9760_001702"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAT5043470.1"
                     /translation="MRQRFVHLRVHTEFSLVDGLVQIKPLMKALPARGMSTVAVTDFC
                     NLFAAVKVFKSATEAGIKPIIGSDLPCHDLETPDNIFSLVLLCQDTAGYKNLTCLVSK
                     AYQEGQYHGQPRVQLQWIPEFSQGLIALSGGKFGDIGQALLANDDTLARKRALHWMNL
                     FPNRFYLEIQRTGRADELHYNEKLIALADELQLPLVATNDVRFIDEDDFDAHEARVCI
                     HEGYTLADPRRVQQYSSQQYLRSADEMELLFSDLPSALQNTVEISKRCNVKLELGHNY
                     LPNFPIPDGTTVEAYLSHLSEIGLEERLKQLFRDKSEGELLAARGEYDKRLKIELDVI
                     NNMGFAGYFLIVADFIQWAKQNGVPVGPGRGSGAGSLVAYALKITDLDPLEYELLFER
                     FLNPERVSMPDFDIDFCMEGRDRVIEYVAEKYGRQSVSQIITFGTMAAKAVVRDVGRV
                     LGHPYGFVDKLAKLIPFELGITLSKAMEQEEELRRRYEEEEEVKELFDLALKLEGITR
                     NAGKHAGGVVIAPSQLTDFTAIYCEEGSTQIVSQFDKDDVEAVGLVKFDFLGLRTLTI
                     IDWALATVNKQCENAGLSPIDISQIPTSDDSTFELLKACKTTAVFQLESRGMKELISR
                     LQPDCFEDIIALVALFRPGPLQSGMVDDFIDRKHGRARVDYPHPDLEPILKPTYGVIL
                     YQEQVMQIAQVLANYTLGAADLLRRAMGKKKPEEMAKQREIFTQGAVARGVDEQVATH
                     IFDLMEKFAGYGFNKSHSAAYALVAYQTAWLKAHYPAAFMAAVMSSDMDNTDKVVTFI
                     DECAHMKLQVLPPSINNSMYHFTVSDEKTIIYGLGAIKGVGESAIECIIQERNQNGPF
                     LDLFGFCQRLDLRKVNRRVIEALIKSGAFDVWKVERAVLTASLEKALKVAEKEHQNQS
                     SGQFDLFSLLEDKADEQNYVESKSWSEAQRLEGEREVLGFYLTGHPADQYRRELGDFI
                     VPVGQLNPSLHKKAIICAQVVAIRKIITKRGKKLVIIGMDDATARIDAVVFGEIFEQY
                     SDPLGTGNMLVIEGEVAIDDYNGGVKMTANQLLSIASARTKFARCLELRLNQNNVAVL
                     PSIQSLLKTYSGRCAVQFVYANGVAKANLNLGQQWCVTPSDELIGLLSNLLGEEQVIM
                     RY"
     gene            complement(12500..13846)
                     /locus_tag="I9760_001703"
     CDS             complement(12500..13846)
                     /locus_tag="I9760_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT5043471.1"
                     /translation="MTFELLWTDKCFLTLLAVVLFTVLLSLRKQHIKLSFAIILQRPL
                     AISAGIVLLLFLAIGVLDSIHIKFINDKTEKTQSLLDRVLAPIGNTYEKTYSAPLALN
                     QFMSETKLVNGVTTQVYPRLKYPPASIKNEKDKSLIIKSVIMKGITYSALVILGGCLL
                     LALVKFSLGYKNTFILNSTQISAIATVAVLTFAVVIGYGLSRSFHVLGTGQIGQDIFY
                     FSLKSVRTGLIIGFLTTLFMLPLALFLGIAAGYFGGLVDDIIQYVYTTLSSIPGVLLI
                     TASVLSLQTYIANHPEQFTTLAKSADARLLALCLILGVTSWTSLCRLLRAETLKLREI
                     DYVLAARALGSSWFTIVRKHLVPNVMHIVIITLVLDFSFLVLAEAVLTYVGVGVSPMT
                     ISWGNMINGARLELARDPVVWWPMFAAFLLMFLLVLAMNLFADAVRDAFDPHQSHL"
     gene            complement(13870..>14698)
                     /locus_tag="I9760_001704"
     CDS             complement(13870..>14698)
                     /locus_tag="I9760_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT5043472.1"
                     /translation="IEDWKVVHGYDLPLFYNEKQTGFNQFRQTLFYQKSLMLFTFNFG
                     VSDAGRDISHDISYRMWPSLAIALPTLLFAVLVNIIFAMAMAFFRSTYLDLSGVVICI
                     ILMSISSLFYIIGGQYLFGKILKWFPISGYDDGLDALKFTILPVLVAVLGQLGAGARW
                     YRTLVLEEINKDYVKTARAKGLSEQRILFKHILKNAMLPILTGIVVIIPTLFMGSLVL
                     ESFFGVPGLGSYIIDAIQQQDFAIVRAMVFLGSVLYIIGLVLTDFSYILVDPRVRLS"
BASE COUNT         4945 a         3115 c         2621 g         4017 t
ORIGIN      
        1 ttaaattgaa aatctatatg aaaaaactga aaaaataggc ttcattattt gaattaaata
       61 tttgaaaaca gtaatttttc ttaacaaaat ccatacataa tttactaaat ggaaactttt
      121 atatggcaat accttgagtt ttttacccat tattgatact atttgggcaa ttaattatct
      181 tctatcaagg tgggtataaa aatgggtggc ttaactagcg agcaatatca cagtcaagta
      241 gtcggtaaaa tcggatatat cgccagatgc atgcaaacta tagatcctga aaataatttg
      301 aaaaaaataa gagaagatta ccaagatgta ttgatatggg cagagaaaaa ttatcgtttt
      361 gaggaaatat tggaagccag caaatctgga aaatgcccta atgatctgga tgcattaagt
      421 cgacgatcgc taatactcca agaattgctg cggcttgttt catcaattag tccctttaaa
      481 atgaaattag acctgattga gtctcaatat gagaaaatga agcagcatgt gaatttatgg
      541 aaaagtgatt accatgtaaa actaaatcaa ctgaatcaat taacagatta cctcaaaaat
      601 gcagcaccaa ctcctaaaaa tcattttcta agagcaatga cttctgcatt gcaaatgcag
      661 atagcccaaa ctgggattac agaagataat gaaggcatca accagttgtt taaactggga
      721 ttgcatttgc tcgcaatggc taacgaaaaa ataaatgagc aatatgacct gtttaaaggg
      781 tatgtaaaag atcagcctga agaatctcct tttgaaggca ttttgcctgc ggaagaccag
      841 aaaattttag ttaaagctat gatagattat gccatgccga agctttcaag caaagtttta
      901 caagataaat tatcggcgct atccagttcc gatgtattaa caaaaacatt acttgatagc
      961 atcgacagaa cagttgagga aaacgaaaaa ttaaatgctt taagcaaagt caaattaggt
     1021 aaatttggcc tggatatcag ggaagttgaa gagatttact ctcaagctct gaaaatatca
     1081 ccacaagatg ctttgcaata taccgctcaa caatgtgatg cccaattatt aagcatggca
     1141 tttcctgatt cccaaaatta cattgttgaa tctatatcgg acaaaaaggc caaggccatt
     1201 gcagagctaa tacatagtaa agaatttatt tatcaaatta ttaagacaga agtttttaaa
     1261 caagttgatc ctaatgagaa aattcaacta caagctgcga cagaattgta tcagcttctt
     1321 gggagaataa tggataaaca gatccatctt tttgccaaga tgaatctcga acaaattaat
     1381 gaatatattc aaaccaaaac caaagcaatc ctggataaaa ttcctgaaag agttgagtta
     1441 ttaactttta tgggctttga aattccaact ttcaagggaa ttgagacact tatgaccgac
     1501 gtctcccatt ctcaggataa tgaaactctg gcgatagccc aagaatttta tgcaaatata
     1561 aaaaatgcaa agaaacaatt gctgggtgat aaactgattg aagacattac tccacaggat
     1621 gtagagaaat tttttaatca atgtagtcaa tacggttcag aagcagctga aaagttagct
     1681 gacaatcgtc cggcattgac aaaaatagcc gatattttga cagcaattgc ccgctgggcg
     1741 attagtttaa taggtttcaa cacgccaccg cagtttttgg ctccaaccag aacctgtgtg
     1801 gatcaggtat ctgatgaaat cactaaaatt aaactcaaac ttgaagatac tttgggaagc
     1861 ttgcaaaaag tacaggaaga gaacctaagc ctttaataac gattaagtcc acaataaaaa
     1921 aatacttgat ataagaattt acctgttatc agagatgttc tcccggatta cagctttcct
     1981 gtaatccgga gcgcccttta taatgagatt atgcatcata tacctgctaa tgggtctggt
     2041 ggttagcaca gattttaccc atccctcccc ccagaaatct tccacgaaat gcatcaccat
     2101 actacctaag ctcatggggc gagagagcac atcgaagtga aattaaaatc cctgctcaat
     2161 tcaagactaa gtgttcaata aaaacttagg gatattgatt tagtattcac cacaaccaat
     2221 tggtattatt attgtcattt tgaatagttg taaaccaaat gctacctttt aaatataggt
     2281 atatgagtgg agcagatatc atgactgggt caagtagtga acaacttgag gaagaatacc
     2341 ttaacgcaag gagagagtat tgtatggcaa tgatggccgt acacgacaaa gcaagggcat
     2401 ttctgagtga aactccaata gatgaaagca tgaagcaagc tataataaaa tcaagcgggg
     2461 taaaaattcg gcctaatgaa acacaagatg aattcgatga gcgcttcgag gacgaaatgg
     2521 gtttaacgat agatgactac gttgattttg cgcttgatgt attggaaaaa aaattggaac
     2581 ccgcaaagca gagtattgta gctgctgcaa ataaagaact tggttctctt acttctggac
     2641 aatctggttc ggcatgggaa gattttcaac atacgatcca cgagggagca gaatataaaa
     2701 ctatagaagt ggctaagtgt ctccctgagt ctgagcgttg catacaagca tttaatcagg
     2761 cgaagctggc atttcgtgat gcagccacaa atttaataca tgcggagaaa ctggacgaac
     2821 aggaagagcc aaatcacggt cccaagttct aaaacaaggg cccaggaaga tgtaagaagc
     2881 taagaaattc ttttgctgtt tttcatttta ccatcaaatt atgagaaaat tgtgtttcaa
     2941 aatcaacata agtaattgat ataacagcat cttgatttac cagattaggt ctattgctta
     3001 agcttcaacc ataggttcca attgaaactc aggcaccaga ctttttaaca gaacgagtaa
     3061 ttcttcatcc tgatgctcaa tacaagctgc ctgcaacatt cgcatggttt gtgtcaaatc
     3121 attccaatcc agttcacgaa accttgcttt gaataatttt tcatgttttg ttgaagttaa
     3181 ctgttctgtc tcatgaaaca actcttcata cattttttca ccaggacgca accctgtata
     3241 ttcaatcatg atgtctcttc ctggttcttt gcctgccaag cgaatcattt gttctgcaag
     3301 ataactgatt ttaaccggct ctcccatatc cagaacaaaa atttcaccgc cctccccatt
     3361 caccatcgct tgcaaaatca actggcatgc ttccggtata gtcataaaat atctttgcat
     3421 atcgggatgg gtcacagtca gtggaccacc ttcctgcaac tgtttttgga acaaaggcac
     3481 cacactacca gctgaaccca agacatttcc aaaacgaacc gtaataaact gggttttaac
     3541 tcgtgcattt aaattctgac aataaatttc agcgactctc ttggtagttc ccattatatt
     3601 cgttggattc actgctttat cagtggaaat taaaataaat ttctcagcgc caaatgtcac
     3661 actggctttg gcaacaactt gagtacctat gacattatta aacacagcaa cccgtacttg
     3721 atgctgaagc atgggaacat gtttgtatgc agccgcatga aatacaattt caggctgaaa
     3781 ccgacgaaat aaatgattaa tcgcaatttc gtctgtcaca ctgattaaac tcaattcaac
     3841 ggggatacct ggaaaactct ttaacaactc ctgttcaatg cgatacaggt taaactcact
     3901 attctcaaca atggctatgc tcgcaggctc taaggccata acctgacggc acaattctga
     3961 accgattgag ccaccaccgc ctgtaatcaa tatacgtctt cctgcgattg aacgagcaat
     4021 tttttcccat tcgagcgtca cttgatcccg acccaataaa tcctcaatat tcacaggcct
     4081 cagcgcattc acttcaactc gacccgcagc cagggctgaa atactgggta aagtactaaa
     4141 aggcacatgg ctttgttcac aaagcgttac aattcttcgc atggttgccg atttggcaga
     4201 aggtatggca atgaaaatca aatccacgtc gtattgattg actaattcgg taatttggtt
     4261 ggtcgttccc agaaccctca ctccatgaac ttctaatcct ctcttgcttt tattgtcatc
     4321 tacaaagcct attggccgat aataactgct tcttttcaaa tcacgcacca agccttcacc
     4381 agcctgcccg gcaccaacca ccaaaactct tttcgtttcc aattccctat ttcctctgtc
     4441 aggcttatcc caatgcagac ggatcaccaa cctacccccg caaaggatgg tggccaggat
     4501 aatgcaatac aaaggaaaaa ctgatctggg caggtgctga agaattgaag tcaagtaaaa
     4561 aacagggatt actaaaacca ttgctgtgat agtagccttc agtatcctga tgacatcatt
     4621 cagagaagag aaacgccata atcctctgta aattttaaaa taatagtaac aactaatctg
     4681 aacaattgat aataaagcta aagcaataaa agaatgggtg gatgtcaggc tgccgggata
     4741 tggctgcatg ttatagcgca accagtaagc ggcataccag gcaactggta ttgcagccac
     4801 atcaaacaaa attaccggga gttttgcaat gatttttttt gtcaatatga ttattttttg
     4861 cattataaaa accacagtct gtaatccttt ataaaggaaa gatggtacac tatgttacag
     4921 tttcccgcat caaaaattag caaacaattc atacctatat tgtgactcag cacttccaaa
     4981 agaaaaaaca ccaaccaact tgtattcttt atgaatatca agaaatggtt tcaaacaaga
     5041 atcaccggga gtataaatga tctttccttt atcaataagc gcagtcaaaa taattatctg
     5101 tggtttttct tctaaaatct gctggcaaaa taattcatat ctcttcacgg caggaaattg
     5161 tgccatttct ttattattca ggcagtaaga atcaataaaa gttaatccgc ttaaaaacgg
     5221 aatcaaacca gaatctgcca acaccactct atcttgattc ttggcatgag tatttaacca
     5281 ctggacaaca cttttccgta attgctctcc atgtaccgga ttttgactaa agtagcgata
     5341 agcatcaagg gacattttgg gtataaatag tagcgccaga aaaaatgaac tcacatacaa
     5401 tgccgctcta aagtaattat cttgtttttt gaaatagctt aataaaagat aactgatacc
     5461 attcaaggtc aggggtaaca taagagcaaa cgctggcaag aacaatcgat tatcaaaagc
     5521 gacaactgga tcggcattca caagcattaa taaataaata ataccgggag accataaaaa
     5581 ataatgcctt ctgtcctgag cataaaaaca ggctggcaaa gccagaatag caaatggcca
     5641 aattaatttg agataattca aatccaatga ataggatatg ccattaaaac ctttacaata
     5701 tacagagtta ggaaacaata acccgtaata actccaacgc caaagaaaat aaggcagaaa
     5761 caccaaacct atggtcaccg caaaagatac cgcccctctc catggcttct tgcctgataa
     5821 tttattccag tcccaaccga ttaaaagaaa aaaaagaagc atcagggctg gtgtttcagg
     5881 cctggtcatt cctgcgaatg aaagatacaa cccagacaag acaaaaccac ttgtcttaaa
     5941 ttcacctcga ggagcgggat aaaaatgata tcctaacccg cgaaaagcaa aatagactga
     6001 accgctaatc agtgcctggt aaacggtcgt ttcaaggccg ctaactgacc aaataacttg
     6061 tcctttgtat agtaaaagcc aaatgcaggg gataaaagac tctcgtttgg taaaccaaaa
     6121 gcggctgaca agatagataa aaagacacgt ggcaagtaat ccagcaaacc cggcgatttt
     6181 tagggtaaga accggattaa tgtgcagaat aattgatatt gcccccaaag caacaaagct
     6241 gaaattggaa tagccttcaa ccggctcagc gtttaaattc cataataagc catttcctgc
     6301 agcccagttt ttggcatacc gcaaagaaat atacatgtca tctatagtga acggccatat
     6361 ggcctggatt tgtaaaacca gcaaataagc aaataaaacg ataaacaggc tgtcatacca
     6421 gcgccactta gcactcatca attcaatgcc ataaaggtca ggatagcgaa gcaccataaa
     6481 ttaattcgag ataatcgatc acttaaaaaa atattgaccg gatcatcatt ctctacatcc
     6541 tgagtggcaa gccaggcaaa acgccaaagt gcaatagcgg caaaaggtaa agttaaaatg
     6601 aaaaaaaacg attctgctct ggcataaacc gtgtaaaaca gataagtaat aaagcaggct
     6661 attcctgtaa tgacgataag tctttgcaga aaggctggat tatatcggcg tagaacctta
     6721 cgggttgaat ttttcaatcc taactgcatt tccagttgcc ttttatttag ggcgataaat
     6781 aaactaagca aagtcgctgc aacagttaac caggctgata tagccaaatc aattcctatt
     6841 gttccggcca aaactctgag cataaatccc aaggcaatac aagcgacatc aaaaaccgga
     6901 atcaatttta aaacatggtt ataagcaaga ttgattatca aataaataat taaaatgacc
     6961 accagttttt ttgaaataag ccaacccagc gcgattccag caaccatcag taaaagcatt
     7021 acaatgatcg catcaaataa agtgacctgc tcgctggcta taggcctgtt acatttaaag
     7081 ggatgcagac tgtcttccgt tctatcctga atatcattat aaatataaac agcacttgca
     7141 ataaggcaaa aagataatga agccattaat gccggaaaca aataccccgg aacaggggta
     7201 taaatgaaac cgagcataac aaaaattgct ttactccagt gcgatagacg aagaagtaaa
     7261 agatattggt ataacttatc catgtaactt accctgatga ttttatttgc taattccatc
     7321 atacaaatct acccttttct caaaaacagc taaaacagtt ctgtcatttc taatcaaagt
     7381 attaatgacc gcctcaatca ggctacagga catggatttg ttttaatttt aaattaatta
     7441 caccctataa gagtaacata tctaactcaa ggctaaaaat agtataatcg caaaaaaatc
     7501 atcatttcaa agagttaaat cataaataaa atctgtagcc ttatctgatt gcaacaattt
     7561 agtaaaaatt aaccaaatca gattgcattt aaccagctaa tcggtaagat aaaaaacaca
     7621 caaataaatt ttcaatggat atgaggttat tatgcgctat atactgccag caggagctct
     7681 aaccagcctt aactttttac gcgatgacta tcaatcagaa agaaaagtag atttggatca
     7741 acccccatct ttaatcggag tacctactct ttttggaggc actgatgtca tagccagaga
     7801 taaacaaacc aaatttataa aaaaaatgat tgttgtcctt agagcaaatc tggcgaaaga
     7861 ggaagaaatc acaacccctg aacaatggga agcgaattta actgcttcca gagtcgtcat
     7921 cgcagcctgt ctatatgttc aatcgcaaat ttcatctccc aaaaataact ccgcattata
     7981 tcgattgatt aataaggatt taggcattac tgataccaat tatctggatg atgaagacaa
     8041 ggaaatctgc tttttagctg ccaatagagt ggtcaactcc tctttatccg catttagcga
     8101 tgccaacgca gccttgcgca aagccaatat gaagccattt accgagcagg agtggagtga
     8161 cttttccaag tttgtcagta gtgtaagtgt taaaaaggta tctaccaatc cgtactcgaa
     8221 ctatcccgtc acatccatta ctcaaccgtt attcggagct gctttttctt atacaggagc
     8281 aacgattggt tatcttgggg gggatgttat tagccaatcc tccaaagcga tgtcgaccaa
     8341 actgcaaatt actaccatgg tgggaagcag cctgctaatg cttggtacaa caggacctat
     8401 gggcgtggcg ttgttctctc aggttatagc gagtaaatta gtcacggcat ttttcagtat
     8461 tagtttggct catgttttag gaacaaccat gggtattctc ggacaaggcg taggaatggg
     8521 catcgggtta cctttggatt atgcctacag attaatatgg aaagccagcg ccactattat
     8581 gaattactac agcaactatc ctaatacctc tcaaatgacg ggtattcgta ttgcggatgg
     8641 caaatccatc atgaatggca tagtgattga atacactcca agctctgatt taaaaactga
     8701 agaaggacag caaacagttg aaattactga agaaggaaaa cttcttatca atggaaaaga
     8761 agtcactatt cctgacggcg gagttcaact tccactagag ttaattaatg aattaaaagc
     8821 tcaattgaag ctacatgaag aaaaagttag cgaagaagac tcttcctctt ccacattgct
     8881 agcaactgtt tgatgtagat gagacaacta tcattctgca atgcgagttg agccgtgcta
     8941 tctgctcaac tccacaggtg ctatcattag cagtatttct aataacgcat tattacttgc
     9001 tcttccccta aaagattgct caatagtcca attaattcat cgctaggggt cacacaccat
     9061 tgttggccaa gatttaaatt agctttagca acaccattag cataaacaaa ttgaacagca
     9121 caccgtcctg aataagtctt cagtaaggat tggatagaag gcaatacagc gacattgttt
     9181 tgatttaaac gcaactccag acaacgagcg aattttgttc gagctgaagc gatacttaaa
     9241 agctgattgg cggtcatttt aacaccaccg ttgtaatcat caattgcaac ctcgccctca
     9301 attacaagca tattacccgt ccctaatgga tcagaatatt gttcaaaaat ctcaccaaaa
     9361 actacggcat caatcctggc agtcgcatca tccattccaa taatcactaa tttcttgcca
     9421 cgtttggtga taatttttct aattgctaca acttgagcgc aaataatcgc ttttttatgt
     9481 aaagacggat ttaattggcc tacaggaaca ataaaatctc ccaattcacg tctgtattgg
     9541 tcagcaggat ggccagtgag gtaaaaacct aatacctctc tttcgccttc taatctttga
     9601 gcttcagacc aggatttact ttccacataa ttttgttcat ccgctttgtc ctctaaaagc
     9661 gagaatagat caaattgtcc acttgattga ttttggtgtt ccttttcggc aactttcaat
     9721 gctttttcca aggacgctgt taatacagcc ctttccactt tccaaacatc aaaagcacca
     9781 ctcttgatta aagcttcaat cactcttctg ttgactttgc gtaaatccaa tctttgacaa
     9841 aaaccaaaca aatccaaaaa aggtccattc tggtttctct cctgaataat acactcaatc
     9901 gcagattcgc caactccctt tattgcccca aggccataaa taatcgtctt ttcatcggac
     9961 acggtaaaat gatacatgga gttattgata gaaggaggca acacttgtaa tttcatgtgc
    10021 gcgcattcat caataaaagt tacaacctta tccgtgttat ccatatcaga ggacatcacc
    10081 gcagccataa aagctgcagg ataatgcgct tttagccagg cggtttgata agcaactaaa
    10141 gcgtacgcgg cagaatgtga tttattgaat ccatatccgg cgaacttctc cattaaatca
    10201 aaaatatggg ttgctacctg ctcatctact cctctcgcaa cagctccctg ggtaaaaatt
    10261 tccctttgct ttgccatttc ttccggtttt ttcttcccca tggctcgtct taacaagtca
    10321 gcagcgccga gcgtgtaatt ggcaagaacc tgagcgattt gcattacttg ttcttgatag
    10381 aggataaccc cataagtggg tttaagaatt ggttccaagt ctggatgggg gtaatcaact
    10441 cgagccctac catgcttcct gtcgataaag tcatccacca ttcctgactg tagaggacct
    10501 ggtctgaata aagcaaccaa agcaatgatg tcttcaaaac aatcgggttg aagccggcta
    10561 atcagctcct tcatgcctcg agattccagc tgaaatacag cagtagtttt acatgctttt
    10621 aataactcaa aagtagagtc atcactcgtt ggaatttgac taatatcaat tggtgacaac
    10681 ccggcatttt cgcattgctt attcactgtg gccagagccc aatcgattat ggttagtgtt
    10741 cttaatccta aaaaatcaaa cttgactaac ccaaccgcct caacatcgtc tttatcaaat
    10801 tggctaacta tttgtgttga tccttcttca caatatatcg ccgtgaaatc agttagttga
    10861 gaaggtgcga taactactcc cccagcatgc ttgcctgcat tacgagtaat tccctccagt
    10921 ttcagggcaa gatcaaatag ttcttttact tcctcttctt cttcatatcg cctgcgaagc
    10981 tcttcttcct gctccatcgc tttacttaaa gttattccta actcaaaggg aattaatttc
    11041 gcaagcttat ccacaaaacc ataaggatga cctaaaacac gcccaacatc tctcaccaca
    11101 gcctttgcgg ccatagtgcc aaaagtaata atttgggaaa cgctctgccg gccatatttc
    11161 tctgctacat attcaatgac ccgatcacgg ccttccatgc aaaaatcaat atcaaagtca
    11221 ggcatagaaa cacgttcggg gtttaagaat cgttcaaaaa gcagctcata ctctaaagga
    11281 tccagatctg ttatttttaa ggcataagca accagagaac cagcaccaga accacgacct
    11341 ggccctactg gaaccccatt ttgtttagcc cattgaataa aatcagcaac aatcaggaag
    11401 taaccagcaa agcccatgtt attaataaca tccagttcaa ttttaaggcg tttgtcgtac
    11461 tcccctctgg cagcaagcag ctccccctca gacttgtctc tgaataattg ctttaaacgt
    11521 tcctccaggc caatttcaga aagatgtgat aaatacgcct ctactgttgt tccatccggt
    11581 atagggaaat taggtagata gttatggccc agctcgagtt tcacattaca gcgtttacta
    11641 atttcgactg tgttttgtaa tgcactggga agatccgaaa ataagagctc catttcatct
    11701 gctgagcgca gatactgttg agagctgtat tgttgaactc tcctgggatc agccaacgtg
    11761 tacccttcgt gaatgcatac acgagcttca tgagcatcaa aatcatcctc atcaatgaac
    11821 ctaacgtcgt tggttgcaac caacggtaat tgcaattcgt ccgctaacgc gatcagcttc
    11881 tcattataat gcaactcatc agctcttccg gttcgctgaa tttccagata aaaccgattc
    11941 ggaaataaat tcatccaatg caaagcacgt tttcttgcca aagtgtcatc atttgccagc
    12001 aatgcttgcc caatgtcacc gaactttcct ccggataatg cgattaatcc ctgtgaaaat
    12061 tctggaatcc attgtaattg caccctgggt tggccatgat attgcccttc ctgataagct
    12121 ttggatacca gacaggttaa atttttatag cctgctgtgt cttgacataa caaaaccaaa
    12181 gaaaaaatgt tatccggggt ttccaaatca tggcatggca aatcactacc aataatgggt
    12241 ttgattcccg cctctgttgc acttttaaat actttaacag cagcaaataa attacaaaaa
    12301 tccgttacag caactgtgct catacctctg gctggcaaag ctttcatcaa tggtttaatt
    12361 tgtaccaaac catcaactaa tgaaaattcc gtatgtactc ttaaatgaac aaaacgttgt
    12421 cgcatagaga attaacccat tcacgataat ttcgaaactt taacctaaat cccggaaata
    12481 ggaaactaac agatattgac tataaatgag attgatgtgg gtcaaaagca tcccgtactg
    12541 cgtctgcaaa caagttcatt gccaaaacca ataaaaacat taacagaaac gctgcaaaca
    12601 taggccacca tacaacaggg tcacgtgcta attcaaggcg cgccccatta atcatatttc
    12661 cccaactaat agtcatgggt gaaaccccta cccctacata ggtcaataca gcttctgcca
    12721 agactaaaaa actgaaatcc aataccaggg tgatgataac aatatgcatg acattaggca
    12781 ccaaatgttt gcgtacgatg gtaaaccaac tactgcctaa ggcacgtgcg gccagaacat
    12841 aatctatttc tcgcaatttt aaagtttctg ctcgcaaaag tcggcacagg cttgtccaac
    12901 tggtcacacc cagtatcaag caaagagcta ataaacgtgc atcggcactc ttggccaaag
    12961 tagtaaattg ctccgggtgg tttgcaatat aagtttgcaa tgacaatacg gaagccgtaa
    13021 ttagcaaaac acccggaatg gagcttaaag tagtataaac atactggata atatcatcca
    13081 caagcccacc aaaatatcct gctgcaatcc ccagaaataa agccagtggc aacatgaaga
    13141 gggttgttaa aaaaccaatg attagacccg ttcgcacact tttcaaactg aaataaaaaa
    13201 tatcttgtcc gatttgccct gtgcccaaga catgaaagga tcttgataat ccgtaaccaa
    13261 tgacgaccgc aaaagtaagt acagctacag tcgctatagc gctgatttga gtagaattta
    13321 aaatgaaagt atttttatat ccaagactaa actttactaa agcgagtaat aagcatccac
    13381 ccaaaatgac taaagcactg tatgttatac ctttcataat cacagatttg atgatgaggg
    13441 atttgtcctt ttcattctta atagaagctg gtggatattt taagcgagga taaacctgag
    13501 ttgtaactcc attaaccaac tttgtttcac tcataaattg atttaaagcc aagggagctg
    13561 aataggtttt ctcataggta ttccctatag gtgcaagaac tctatccaat agagactgag
    13621 ttttttctgt tttgtcattg atgaatttaa tatgaatgga gtccaaaaca ccgatggcta
    13681 aaaacagcaa caacacaatt ccagcactga ttgctaacgg cctttgtaaa atgattgcaa
    13741 atgacaattt gatgtgttgt tttctaaggc tcagcagaac ggtaaataaa accaccgcca
    13801 gtaaagtaag aaaacatttg tcagtccaaa gcaattcaaa tgtcattttt gccacatact
    13861 ccttggttat caggataatc gcactcgggg atcaactagg atgtaagaaa aatcagtcag
    13921 aaccaagcct atgatgtaaa gaactgaccc cagaaatacc atagctcgta caatagcaaa
    13981 atcttgctgt tgtatcgcat caataatata gctacccaag cctggcacac caaaaaaaga
    14041 ctctaaaacc aaacttccca taaacaaagt aggaatgatt acaacaattc cggtcaaaat
    14101 gggtaacatg gcatttttta atatatgctt aaataaaatc ctttgctcag agagcccctt
    14161 tgctcttgcg gtttttacat aatctttatt aatttcttcc aaaaccaagg ttcgatacca
    14221 acgcgctccc gcacccaatt gtcccagaac agctaccagg actggtaaaa tagtaaattt
    14281 taaagcatcg aggccatcat cgtagccgga aatagggaac cattttaata tttttccaaa
    14341 cagatattgc ccgccaatga tgtaaaacaa actggaaatg gacattagga taatacaaat
    14401 aactaccccg gataaatcaa gataagtcga acggaaaaat gccatagcca tagcaaaaat
    14461 gatattcacc aaaacagcaa ataataatgt tggcagggca atggcaaggc ttggccacat
    14521 tctgtaggat atatcgtggc taatatctct accagcatcc gagacaccaa aattaaatgt
    14581 aaacaacatc agcgattttt gataaaacaa agtctgtcta aattgattaa atccagtctg
    14641 cttttcatta taaaataacg gtaaatcata gccatgaacc actttccaat cttcaatg
//
LOCUS       DACTJE010000074        14192 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.074, whole genome shotgun sequence.
ACCESSION   DACTJE010000074 DACTJE010000000
VERSION     DACTJE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.074"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..476
                     /locus_tag="I9760_001705"
     CDS             <1..476
                     /locus_tag="I9760_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HAT5043473.1"
                     /translation="NKNNKHPQKLAIVLDIDETSLSNYDKMVKRDFTGSKEQIHKEIL
                     AANSPAIKPMLTLYKNALKQGIKVFFVTGRQESERDATRANLIKAGYTKWAGLYLRPN
                     GYSSPSIIPFKSKAREMIAKKGYTIIASIGDQCSDIQGGYTKKGFKLPNPFYYLP"
     gene            complement(578..1639)
                     /locus_tag="I9760_001706"
     CDS             complement(578..1639)
                     /locus_tag="I9760_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HAT5043474.1"
                     /translation="MSKLINLAVLSFSSSLAFAGPLTLKGEVDVHITPKNPSGVAQSL
                     TFKLPKYELSTEAKSYLREQLSEYPKNSINTASFSSELPRKVKLGMQLTPVLDQGYHG
                     SCVTFAVTAAIDAALGAGDYISQLCNLELGSYLAIHDKAKASGWNGSFGYWVLQQISE
                     YGIISQNYQKLNGCAGVREYPLEDENNEGKPMSDSEFLAHSVPVSNLISWEALLKDEE
                     SFSAKADMNQIVYQIKEELAKGNRLTIGMLLDVFVGDAGAVGTNRAYNDTWMLTPEIV
                     LDAMNGMIYAGHELVITGYDDDLEVMDEEGHVNKGVFTLRNSWSKFAGDQGDYYVTYD
                     YVKFLAMEVMAIRMKEKAA"
     gene            1949..2761
                     /locus_tag="I9760_001707"
     CDS             1949..2761
                     /locus_tag="I9760_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HAT5043475.1"
                     /translation="MRTLLSCFLITLLSFGLIINEASAKRFGGGRSFGVQRSQSSLFS
                     SNKTHNTSSLTQKANKSRWGGVLGGLLVGGLLASLFMGNGLANGLITWLILGSVIFFI
                     ISFLRRKMNPGFQATQSGAFRQNSFEQFTQNYGNRNSGGAHFAQQETSFDADNFLRDA
                     KITFIRLQAAYDQKNLQDLQTFTVPEVFAEIKMQLDERGDAPNQTEVISLDAKLLDVS
                     KQSLSMIASVQFTGTIKENGEISNLDEIWHFRQFDNRKEWVVGGIQQEVFQP"
     gene            complement(2836..3318)
                     /gene="greA"
                     /locus_tag="I9760_001708"
     CDS             complement(2836..3318)
                     /gene="greA"
                     /locus_tag="I9760_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAT5043476.1"
                     /translation="MSKHPMTVEGAEALKAELHRLKFVDRPRIVEAIATARAHGDLKE
                     NAEYHAAREQQSFNEGRIQELEAKLSHAQIIDISKLPNNGKVIFGSTVTICHVATGSE
                     LTYKIVGEDEADIKLNKISYSSPIARALIGKELDDAVTVETPGGMVEYEIIQVQYIVE
                     "
     gene            complement(3327..6530)
                     /gene="carB"
                     /locus_tag="I9760_001709"
     CDS             complement(3327..6530)
                     /gene="carB"
                     /locus_tag="I9760_001709"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAT5043477.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGTQAVRALKEEGYRV
                     ILVNSNPATIMTDPELADATYIEPVQWKEVARIIEIERPDALLPTMGGQTALNCALDL
                     VREGVLAKYSVEMIGATREAIDRAEDREKFRQLMIKIGLDMPRSAIAHSLEEAIQVQA
                     RLGFPAIIRPSFTMGGSGGGIAYNREEFEEICIRGLELSPTHELLIDESVLGWKEYEM
                     EVVRDKNDNCIIVCTIENFDPMGVHTGDSITVAPAQTLTDKEYQRMRDAAIKVLRAVG
                     VDTGGSNVQFAINPEDGRMLVVEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNEITGGKTPASFEPSIDYVVTKVPRFNFDKFPQTPDTLTTQMKSVGEVMAIGSNFQ
                     ESLQKAIRGLEIGRCGLHSLFQKEDMARLRGHLREPTPDRLWYIADAFRQNLSLEEIH
                     LESRVDPWFLAQIQELVLLERSIIGKTIRDLDKTALHLMKHRGFADAYLAKLLGCTEA
                     EVRARRVELEILPVYKRIDSCAGEFPSDTAYMYSCYEGSCEARPESNKKKIMILGGGP
                     NRIGQGIEFDYCCVHAALSLRSAGYQTIMVNCNPETVSTDFDTSDRLYFEPVTLEDVL
                     SIVAMEQPEGVIVHYGGQTPLKLARDLEANGVKIIGTSSDAIDMAEDRERFQQMVKEL
                     KLHQPDNGTVRSEAEAIELANKIGYPLVVRPSYVLGGRAMEIVYQEEDLRYYLSHAVE
                     VSNDSPVLLDKFLNDAIEVDIDAVCDGKDVLIGAIMEHIEQAGIHSGDSACTLPPFSL
                     GVVVQQDLIEQIRKMALKLGVVGLINAQFAIQADDIYVLEVNPRASRTVPFVSKATGL
                     PLAKIAALCKVGISLKDQGLNQNHHTPSFYSIKLPVFPFIKFSGVDSILGPEMKSTGE
                     VMGIAKRFGQAYAKAQLGAGCHIAKRRRAFVSVRDADKTRVGEIAKRLIELGFEIIAT
                     RGTALALQAAGVDCRRVFKVAEGRPHVLDFIKNNEIDFIVNTTEGKQAIADSFAIRRN
                     ALQHKVSYTTTLSGAEAACLAMKYEDRETVTRLQDLH"
     gene            complement(6657..6929)
                     /locus_tag="I9760_001710"
     CDS             complement(6657..6929)
                     /locus_tag="I9760_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043478.1"
                     /translation="MNEMDILSLFYDEMTARGVTREQVFLSIEEDAAAMLTQKLGKPV
                     SVEEAQKLTDICIANEWLERTTADPYYKYLSLTEAGLQMLLSTLYK"
     gene            7042..8223
                     /locus_tag="I9760_001711"
     CDS             7042..8223
                     /locus_tag="I9760_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAT5043479.1"
                     /translation="MQEIDVLIIGAGAAGLMCAIEASKRKRKVLVLDHANKAGKKILM
                     SGGGRCNFTNYYIEPNKYFSHNPHFFKSALSRYTQWDFIELVNKHKIPFHEKTLGQLF
                     CDNKSKDIVDMLLKECEQYGVAIYLNTVIEKIQKTNDYSFKIGTTKGKFHCHSLVIAT
                     GGLSIPTMGASPFAYKVAEQFAIRVWPTRAGLVPFTLDVLEKDRLSILSGIGIDSLVN
                     NERNQFREHILFTHRGLSGPAILQLSSYWNPGESICINLLPEHNLLESLKTARAEAPH
                     KQLNSVLSMYLPKRVVEVFIPQKLGEKKLADSSNKDLETISHLLQNWVVKPNGTEGYR
                     TAEVTIGGVDCHAISSKTMEANNVPGLYFIGEALDVTGWLGGYNFQWAWSSGWAAGQV
                     V"
     gene            complement(8258..9010)
                     /locus_tag="I9760_001712"
     CDS             complement(8258..9010)
                     /locus_tag="I9760_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2628 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043480.1"
                     /translation="MAFIRHLLEYLLHPFFLCLLALGICIFLLYRRLAPTFTKVMLFV
                     VLIVLLAMSTGWLPSYLTYQLESRYPIVKRVNPNVKWVVVLGGGQNIGEEMPANDLLT
                     SASIKRLIEGVRLFRTLPDAKLLLSGGGSAGEQSEALLLTELAKWFAIDSEKVVVETV
                     SINTADQARELAAIVHDQPFYLVTSAIHMPRSMALCQQQGLHPIAAPTDFTFFWSTDT
                     WVKMVIPNSYNLAYTSIALHEILGKIWALGFR"
     gene            complement(9010..10080)
                     /gene="lptG"
                     /locus_tag="I9760_001713"
     CDS             complement(9010..10080)
                     /gene="lptG"
                     /locus_tag="I9760_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAT5043481.1"
                     /translation="MSRKILDRYIAKTVLSAIGLVTLMLVGLQIFILFVNQIDDLGRA
                     DYGIVQAGVFVLLQMPYQVYLFFPIASLLGCLVGLGVLANHSELVVMRAAGISIGQIT
                     GAVLKASLIVILLITSLGETIVPVMAHYANEYKVSALTGGQALLTPRGVWVRNGNDFV
                     SIASVTHENTLRYVNQFRFDNQHNLKLARQISEVKYVNNSWIAYDVKQTEFAEDHTKA
                     HHFDTLPWDVPVKPEILKITGTNPDEMTLHELNRYLREQQRNHQNVANYKFAFFQRIT
                     QPLNTMVMMILAIPFIFGPLRSSTMGSKLLVGAMVGFGFHILNRFFGPVSTVFQWPPE
                     LAAFSPTLIFSLIGIYLMRRVR"
     gene            complement(10077..11156)
                     /gene="lptF"
                     /locus_tag="I9760_001714"
     CDS             complement(10077..11156)
                     /gene="lptF"
                     /locus_tag="I9760_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAT5043482.1"
                     /translation="MIIFRYLTKEVFLTLIALTAILVLIFMSNQFIQYLNRAASGSIP
                     GFVIMKLLMLELPNLMGLLLPLGFYVALLLAYGRLYAESEMTVLRACGYGPRQLLKHS
                     FAMATVVLVVVAIVMIWASPVIAVERTKLIRSTGIKTLIQTIMPGRFHAINSGQQIFY
                     VQSMNRDHSEAEQIFMAKHSVQDNRLLWDVLWADKAFAEVNPQTGEEYIVLQNGKEYQ
                     GVPGHADYQIAEFASYKSKLPNPTVVMHDDARTAKTRDLWPLNNHDLKKAAELQWRIS
                     IPLMVLTLTLVAVPLSRVSPRSGKYSKLLPAIIIYIFYANFMFVSRDAIATGKIPWWI
                     GMWWVHVAVAILGILFIWRNQVKLA"
     gene            11360..12811
                     /locus_tag="I9760_001715"
     CDS             11360..12811
                     /locus_tag="I9760_001715"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAT5043483.1"
                     /translation="MNYGLTHTPSLTTSECLVLGVFSDLALPDFANAIDNEQQGLIKK
                     LCQRVPEPGNTVWQTDVEGHSLLIIQCGKKEEFSANSLQKRVGEITEALIKQRFSSAT
                     VCLPRLTQESAEWQIEQMIVQIDNLRYQLLDFKTKHAKSHKLESVIFHLPGATEKSLE
                     MAKAIVAGVEFCRDLANMPANICTPTYLGEQAILLSKQFDQISCQVMGPEEIKEMGMG
                     ALLAVAQGSDQPPRLIDVHYHGNKNSAPVILVGKGITFDSGGLSIKPANAMDEMKYDM
                     SGAASVLGVIKACALLKLPINLIGIIASAENLISGSAVKSGDIVTTMSGQTVEIINTD
                     AEGRLVLADALTYAERYNPDFVIDIATLTGAIIVALGNIATGYMTRDEQLAKSIERAA
                     NESQDKVWRMPLDEAYQDALESPLADMINAGFDRSAGSITAACFLSRFTEKYRWAHLD
                     IAGTAWISGKKRNATGRPVPLLIQLLRHVANSR"
     gene            12792..13226
                     /locus_tag="I9760_001716"
     CDS             12792..13226
                     /locus_tag="I9760_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAT5043484.1"
                     /translation="MSPIRVDFYLLASSLPESRWLVACRLIEKAYTKGHTIFVFCDNQ
                     QQAEYLDELLWTFKDDSFIPHNLQGEGPEPPPPVQIGYQKEPRGFTDILLNLSTNIPI
                     FYTRFKRVIELVSNVEHEKEVSRLHYKEYRNKGCALHTHSIE"
     gene            complement(13373..13690)
                     /locus_tag="I9760_001717"
     CDS             complement(13373..13690)
                     /locus_tag="I9760_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HAT5043485.1"
                     /translation="MFVTFSCDAYENITYFENVAKQLLLLMGHGGTIPGAIKSENVPD
                     ALHHLQQGLEKIKSENKLLEDDEDSEPDISLAKRAIPLIHLLQAAEKKGCDVLWSHSK
                     SPG"
     gene            complement(13713..>14192)
                     /locus_tag="I9760_001718"
     CDS             complement(13713..>14192)
                     /locus_tag="I9760_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HAT5043486.1"
                     /translation="LTVEIENTDAEGRLILADALAMACEEKPDLIIDFATLTGAARVA
                     VGTEIAAMFSNHDQLAQDIVAASNKVSDPVWRLPLFSPYEELLNSNIADMSNSSSSSY
                     AGAITAGLFLQRFVTKSIPWVHFDIMAWNAVSKPGKPEGGEAMGLRAVAEYLLQTYG"
BASE COUNT         4392 a         3180 c         2676 g         3944 t
ORIGIN      
        1 tcaataaaaa caataaacac ccacaaaaac ttgccatagt actggatatt gatgaaacca
       61 gcctgtccaa ctatgataag atggtaaaac gggattttac cggtagcaaa gagcaaattc
      121 ataaagaaat acttgctgcc aattcaccgg caatcaagcc tatgctgaca ctttacaaaa
      181 acgccttaaa acagggtatc aaagtatttt ttgtaaccgg aaggcaagaa tccgaacgcg
      241 acgcaacccg cgctaattta attaaagcag gatatactaa atgggcagga ctttatctac
      301 ggcctaatgg gtattcttcc ccttcgatta tcccatttaa atcaaaagca agggaaatga
      361 tagcaaaaaa gggatacacc atcattgcca gcatcggtga tcaatgcagt gatatccaag
      421 gggggtatac taaaaaagga tttaaacttc ccaatccatt ttactacttg ccatagtcaa
      481 ggcgtgatgg gtgattaggt ttttcgtagg tcctgttgat tttgaatcta tgaaacctca
      541 ggcacaacga cccgaggttt cttaataact acaagcatta agctgctttt tctttcatcc
      601 gaattgccat gacttccata gccaggaatt tgacataatc ataggtcacg taataatctc
      661 cttgatctcc agcaaatttg gaccaagaat tacgcaaggt gaaaacgcct ttgttaacat
      721 ggccttcttc atccatcact tcaagatcat catcatagcc agtgatcact aattcgtgcc
      781 cggcatagat cattccattc atcgcatcca aaacaatttc aggtgttaac atccaggtgt
      841 cattgtaagc acggttggta cctacagctc cggcatcccc aacaaacaca tccaataaca
      901 tgccgatagt caagcggtta cctttcgcga gctcctcctt gatctgatag acgatttgat
      961 tcatatccgc tttagctgaa aaggattcct catcctttaa caaagcctcc cagctaatta
     1021 aatttgatac cgggacgcta tgcgccagga actcggagtc agacatgggt ttcccttcat
     1081 tattttcgtc ttctaacgga tattctctaa caccggcgca accattcaat ttctgataat
     1141 tctgactgat aataccgtat tcagaaatct gttgaagaac ccagtaacca aaagagccat
     1201 tccacccgct ggctttggct ttgtcatgta tcgccagata acttcctaac tccagattac
     1261 acaattgact aatgtaatcc cctgccccaa gggcagcatc tatggctgca gttactgcga
     1321 aagtgacaca actaccatga tatccctgat ccaatactgg tgttaactgc attcccaact
     1381 taacttttct tggtaactca gaagaaaaac tggctgtgtt tatactattt ttaggatatt
     1441 ctgacagctg ttcacgcaaa taagatttcg cttcggtcga taattcatac ttgggcaact
     1501 taaatgttaa actttgagca actccactgg gatttttagg cgtaatgtga acatcaacct
     1561 ctccttttaa agtcaaagga cctgcaaaag ccagcgatga agagaaagac aatacagcta
     1621 aattaattaa cttactcata aaatatatcc ctataattaa ccctcttgca tcatatcaag
     1681 aggtgaatta aatgcaaaca aatcatgcga ataagaataa aatgacaagc tagctttcta
     1741 ttcacagagt agaaaccatt ttaaaataag gtacctgttc gccagagcgc tatgataatt
     1801 tttatcaaac aaattctatc ccgaatactg aaataacatc ctctcattga cgaatcaaaa
     1861 ataacaaatc agattcaatt taagaaaaca agcaacttga cttgagttca tatataataa
     1921 gcctgattct aaaaaagagg atattaatat gcgtactcta ttgtcatgtt tcctgataac
     1981 cctattgtct tttggtttaa ttatcaatga ggcctctgct aaacgctttg gaggcgggcg
     2041 cagcttcggg gttcaacgct ctcaaagcag tcttttctca tccaataaaa cacacaatac
     2101 gtcttcatta acacaaaaag ctaataaaag cagatggggt ggcgtattag gcggcctcct
     2161 ggttggagga ctattggcca gtctctttat gggcaatggc ttggcaaatg ggttaatcac
     2221 ctggctaatc cttggttcag tcattttctt tataatcagc ttcttacgca ggaaaatgaa
     2281 cccagggttt caagcaactc aatccggagc attccgccaa aactcttttg aacaattcac
     2341 gcaaaactac ggaaaccgaa atagtggagg agctcatttt gcccaacaag aaaccagctt
     2401 tgacgcggac aattttcttc gtgatgccaa gataactttt atacgtctac aagcggctta
     2461 cgaccaaaaa aatctgcagg atttgcaaac attcacagta ccggaagtat ttgcagaaat
     2521 taaaatgcaa ctggacgaac gaggcgatgc ccccaaccaa acggaagtca tttccctcga
     2581 tgcaaaattg cttgatgtgt ccaaacaatc cttatccatg atagccagcg tacaattcac
     2641 tggaacaatc aaagagaacg gtgaaatatc taacctggat gaaatatggc atttccgcca
     2701 gtttgataac agaaaagagt gggtcgtagg gggtattcag caagaagtat ttcagccata
     2761 aatactttta ggtttcctgg agtagcaact gtctttaatg acagttgcac taaaaatcaa
     2821 agaaaataac tgaaatcact caacaatata ttgcacctgg atgatttcat attcaaccat
     2881 accgccaggc gtttctaccg tgaccgcatc atcaagctct ttgccaatta aagctcgagc
     2941 aataggagaa ctgtatgaaa tcttgttcag tttgatatca gcttcatcct ctccaactat
     3001 tttataggtt aattccgagc ccgttgctac atgacaaatt gtaacagttg aaccaaatat
     3061 cactttacca ttgttaggta atttactgat atcaattatt tgcgcatgag acaacttcgc
     3121 ttcaagttct tgaatacgcc cttcattaaa actctgttgt tctcgagctg cgtgatattc
     3181 cgcattttct ttcaaatcac catgagctct tgcagttgca attgcttcta caatgcgtgg
     3241 acgatccacg aactttaagc gatgcaactc tgcttttagt gcttctgcac cctcaactgt
     3301 cataggatgt ttactcatat ttacccctaa tgtaaatctt gtaagcgagt tacagtttcc
     3361 ctatcttcat acttcatagc caaacaagca gcttcggctc ctgataaggt cgtagtgtag
     3421 ctgaccttat gctgcaaggc gttgcgtctg atagcaaaag aatctgctat cgcttgtttg
     3481 ccctcagtag tatttacaat aaagtctatt tcattatttt ttataaaatc cagcacatgt
     3541 ggtcggcctt ctgctacttt aaagactctt ctgcagtcta cgccggctgc ttgtaatgcc
     3601 aaagcagtac cgcgcgtggc gataatttca aaacccagtt ctattaatct cttggcaatc
     3661 tcgccgacac gcgttttatc tgcgtcacgt acggatacga aggcccgtcg ccgtttggca
     3721 atgtgacatc cggcacccaa ttgtgcttta gcataagctt gtccaaaacg cttggcaata
     3781 cccatcactt ctcccgttga tttcatttcc ggtccaagaa tcgaatccac accagaaaac
     3841 ttgataaagg gaaaaaccgg caacttaatc gaatagaaag aaggcgtatg atgattctgg
     3901 tttaatcctt gatctttcaa gctaattcct actttacaaa gagccgctat tttagctaat
     3961 ggcagaccgg tagcctttga aacaaaaggc acggttctcg aagcacgcgg attcacttcc
     4021 aaaacataaa tatcatccgc ttgaatagca aactgcgcat taatcaagcc caccacgcct
     4081 aatttcaaag ccatttttcg aatctgctct atcaaatcct gctgaacaac tacgcccaag
     4141 ctaaaaggag gtaaagtgca tgctgaatca ccagaatgta tccccgcttg ctcgatatgc
     4201 tccatgatgg caccaattaa tacgtctttg ccatcacaga cggcatcaat atccacctct
     4261 atggcatcat tcaaaaattt atcaagcaga acgggtgaat cattagaaac ttcaactgca
     4321 tgcgacaggt aataccttaa atcctcttct tgataaacaa tttccatagc tcgtccaccg
     4381 agaacataag aaggcctaac aactagcgga taaccaattt tgttagctaa ttcaatggcc
     4441 tctgcttcgc ttcggacagt tccattatca ggctgatgca gtttcaattc tttgaccatt
     4501 tgctgaaatc gttctctgtc ttcagccata tcaatagcat ctgatgaagt accaattatt
     4561 tttacgccat tagcctctaa atcacgagcc aactttaatg gtgtttgtcc cccataatgc
     4621 acaatgacac cctccggttg ttccattgcg acgatggaca acacatcctc taaagtaaca
     4681 ggttcaaaat atagtcgatc ggaggtatca aaatcagtgg aaaccgtttc tggattgcaa
     4741 ttcaccataa tagtttggta acccgcgctc cttaatgaca aggccgcatg cacgcagcaa
     4801 taatcaaatt caatgccttg gccgattcga ttaggtccac ctccaagaat cataatcttt
     4861 ttcttattgc tctcaggacg tgcttcacag cttccttcat aacaagaata catataggct
     4921 gtatcactag ggaattctcc ggcacaagaa tcaattctct tgtagacggg caagatctct
     4981 aattcaacac gtcttgcccg aacttctgct tccgtacaac caagcaattt ggccaagtag
     5041 gcgtcagcaa aaccccggtg tttcatcaag tgtaaagcgg ttttatccag atcccgaatc
     5101 gtttttccga tgattgagcg ctctaaaaga accaactctt gaatttgtgc cagaaaccat
     5161 ggatcgactc gactttccag atgaatttcc tcaagtgata aattttgcct aaaggcatca
     5221 gcaatatacc aaagtctatc cggagtgggt tctcgtaaat gccctcttaa acgcgccata
     5281 tcctcttttt gaaacagcga atgcaatcca cagcgtccaa tctctaaacc tcttatggct
     5341 ttctgtaaag attcttgaaa gttagaacca atcgccatga cttccccgac tgatttcatc
     5401 tgtgtggtaa gagtatctgg agtttgtgga aatttatcaa aattaaaccg tggaactttg
     5461 gtaacgacgt aatcaatgct gggctcaaag gacgcaggtg ttttacctcc ggtgatttcg
     5521 tttttcaatt catccaaggt atagcccaca gccaatttag ctgcgacctt agcaatagga
     5581 aaaccggttg cttttgacgc caaagccgag cttcgagata cacgcgggtt catttccaca
     5641 accagcatgc gcccgtcttc aggattaata gcaaactgaa cgttggaacc tcccgtatcc
     5701 acaccaactg cccttagaac tttaatcgcc gcatcccgca tccgttggta ttctttatca
     5761 gttaatgttt gtgccggagc aacggtaatg gaatctccag tatgcactcc catagggtca
     5821 aaattctcta tagtacaaac aataatgcaa ttatcatttt tatccctgac gacttccatt
     5881 tcatattctt tccaacccag aaccgattca tcaatcaaaa gctcgtgagt tggcgacaac
     5941 tccaatcctc taatgcaaat ttcttcaaat tcttcacgat tataggcaat accgcctcca
     6001 ctaccaccca tggtaaatga aggcctgatg atggcaggaa agcctaaacg ggcttgtact
     6061 tgaattgctt cttccaggct atgagcaatc gccgaccttg gcatatccaa tccgatttta
     6121 atcatcagct ggcgaaattt ttctctatct tccgccctgt ctatggcttc acgcgtcgct
     6181 cctatcattt caacagagta cttggctaat accccttctc ttaccaagtc caaggcgcag
     6241 tttaaggctg tttgtcctcc catcgtcggt aaaagagcat ctggcctctc tatttcgata
     6301 atacgagcca cttctttcca ttgaacaggc tctatatagg tggcatcagc aagctcagga
     6361 tcagtcatta tggttgctgg atttgagtta actaatatca ctcgatatcc ttcttctttc
     6421 aaagcgcgta ctgcttgggt acctgagtaa tcaaactcgc aagcctgccc aatcacaata
     6481 gggcctgctc caagaattag aatagatttt atatcggttc gttttggcat aatgacaata
     6541 aaaaaagaat aaagtgatat attttaccga gttttgccca aagattatac ccttggataa
     6601 catgttttga gttattaatt gccagaaaac atccaacggc attaaataac ttaaaattac
     6661 ttgtataaag tagaaagcaa catttgcaaa ccagcctctg tcaaagacaa atatttataa
     6721 taggggtctg ccgttgtgcg ctcaagccat tcatttgcga tgcaaatatc ggttaatttc
     6781 tgcgcttcct ctactgaaac aggtttgcct aatttctggg tcagcattgc tgctgcatcc
     6841 tcttcaatac tcaaaaaaac ctgttctctg gttaccccac gcgcagtcat ttcatcataa
     6901 aacaaagata aaatatccat ttcgttcata gcaattcctt attattggat actaagatct
     6961 atagcatata tttaatcatc gttcgacatg ataaaattaa atacttttta actaaaaaat
     7021 tggtcataac cgaacctctc tatgcaagaa attgatgtcc ttatcattgg cgctggtgct
     7081 gcaggcctaa tgtgtgctat tgaagcaagc aagcgtaaaa gaaaagtact cgtgctggat
     7141 catgccaata aagccgggaa aaagatactc atgtcaggag gtggtcgttg taattttacc
     7201 aattactaca ttgaacccaa caagtatttt tcccacaacc cccatttttt caaatcagcc
     7261 ttatcccgtt acactcaatg ggattttatt gaactggtta acaaacacaa aattcccttc
     7321 cacgaaaaaa cattaggaca attattttgc gataataaat ccaaagacat cgttgatatg
     7381 ttgcttaaag aatgtgagca atatggagtg gcgatttatt taaataccgt aatagaaaaa
     7441 atacaaaaaa ccaacgacta ttctttcaag ataggcacga caaaaggaaa gtttcattgt
     7501 cattctttgg tcattgctac cggaggactt tctattccca caatgggtgc cagtcccttt
     7561 gcctataaag ttgcagagca attcgctatt agagtatggc caaccagagc cggtctggtg
     7621 ccctttactt tagatgttct cgaaaaagac agattatcca tcctgtcggg aataggtatt
     7681 gatagcctgg tcaataatga aagaaatcaa tttcgtgagc atatcttgtt tacccaccgg
     7741 ggactgagtg gtcctgctat cttgcaactc tcatcctact ggaatcctgg tgaaagtata
     7801 tgcattaatt tgctgcccga acataacctc ctggaaagct tgaaaacagc ccgagcggaa
     7861 gctccgcata aacagttaaa ctctgtatta tcgatgtact tgcccaaacg agttgttgaa
     7921 gtctttattc ctcaaaaact gggtgaaaag aaactggctg atagctctaa taaagatttg
     7981 gaaacaattt cccatttatt gcagaactgg gtagtaaaac ccaatgggac agagggctac
     8041 cgtacagcag aagtgacaat aggcggagta gactgtcatg ctatttcatc caaaaccatg
     8101 gaagccaaca atgtaccagg cctatatttc attggagaag ccctggacgt gacggggtgg
     8161 ttaggtgggt ataatttcca gtgggcatgg tcttccggct gggctgccgg acaagtagtg
     8221 tagcgaattt aaaaaccaag tctcaatgca aatcagctta tctgaatccc aaagcccata
     8281 tttttcccag gatttcatga agagcaatgg aagtataggc caggttataa gaattaggta
     8341 tcaccatttt gacccaggta tcagtagacc aaaagaaggt aaaatcagtt ggggctgcta
     8401 tggggtgcaa tccttgctgc tggcataatg ccatggaacg tggcatgtga atcgctgagg
     8461 taaccagata aaagggttga tcatgtacta tggctgctaa ttcacgcgcc tgatctgctg
     8521 tattaataga aacagtttca acgacaactt tttccgaatc gattgcaaac cacttggcta
     8581 attctgtcaa taataaagcc tctgattgct caccagcaga tcccccgccc gataaaagca
     8641 attttgcgtc gggcaaagtt ctgaataatc ggacgccttc aatcaatcgc ttgatgctcg
     8701 ctgaggtcaa cagatcattt gcaggcattt cctccccaat attttgtcct ccaccgagaa
     8761 cgaccaccca tttcacattc ggattaaccc gcttcactat agggtatcga gactctaatt
     8821 gataagttaa ataactgggt aaccaacccg tactcatagc aagcaagaca atcaatacaa
     8881 caaacaacat aactttagtg aaagtaggag caagccgacg atatagtaaa aaaatgcaaa
     8941 tacctaatgc caaaagacat aaaaagaaag gatgcagcaa gtattcaagc aaatgtctaa
     9001 tgaatgccat tatctgactc ttcgcattaa ataaattcca attaaagaaa agatgagagt
     9061 aggactaaag gcagccaatt caggcggcca ttggaatact gtgctcactg ggccaaaaaa
     9121 tcgattcaga atatgaaacc cgaatccaac catagcgcct accagtaatt ttgaacccat
     9181 ggttgatgaa cgtaaggggc caaaaataaa ggggatagct aatatcatca taaccatagt
     9241 attcagaggc tgggtaattc gttgaaaaaa ggcaaactta taatttgcta cattttgatg
     9301 gttgcgttgt tgctcacgta aatagcggtt caattcatgt aaagtcattt catccggatt
     9361 agtacccgta attttaagaa tttccggttt cactggcaca tcccacggca aagtatcgaa
     9421 atgatgggct ttggtatgat cctctgcaaa ttcagtttgt ttcacatcat aagctatcca
     9481 cgaattattg acatacttta cttcacttat ctggcgagcc agttttaaat tatgctggtt
     9541 atcaaagcgg aattgattta catagcgcag agtattctcg tgcgtgactg aagcaataga
     9601 aacaaaatca ttaccattac ggacccaaac ccctcttgga gtcagcaaag cctgccctcc
     9661 tgttaaagcg gaaaccttgt attcattcgc ataatgcgcc attactggta caatggtttc
     9721 acctaaagac gtgattaata ggatgactat cagagacgcc tttaataccg cccccgttat
     9781 ttggccgatg gatatccccg ccgcacgcat cacaaccagc tcgctatgat tagctaatac
     9841 tcccaggcca accaaacacc cgagcaagct ggcaataggg aaaaacagat agacttggta
     9901 aggcatctgt aaaaggacaa aaacaccggc ttgtacgatt ccatagtctg ctcgccccaa
     9961 atcgtctatc tgattaacaa acaaaataaa gatctgcaaa ccgaccagca tcaaagtcac
    10021 caagccgatt gcagataaaa ccgttttggc aatataacga tcaagtattt ttctgctcat
    10081 gcaagcttca cctgattgcg ccaaataaat aggatgccga gaatggcaac cgccacgtgt
    10141 acccaccaca tccctatcca ccagggaatc ttcccggtag caatcgcatc acgagaaaca
    10201 aacataaaat tagcataaaa aatatagata atgatggctg gcaaaagttt cgaatatttt
    10261 ccagaacgag ggctgacacg actaagtgga acggcaacca gagtcaaggt caaaaccatt
    10321 aaaggaatgg aaatacgcca ctgtaattca gccgcctttt tcaaatcatg attgttcaaa
    10381 ggccataagt ctcttgtttt tgcagtgcga gcatcatcat gcataacaac agtcgggttg
    10441 ggcaatttgg atttatagct tgcaaattct gcaatctggt aatcggcatg acccggaaca
    10501 ccttgatatt ccttgccatt ttgcagcacg atgtattcct cgcctgtttg aggattaacc
    10561 tcagcaaatg ctttatctgc ccacaacaca tcccataaca gcctattatc ctgaacgcta
    10621 tgttttgcca tgaatatctg ttctgcttcg gagtgatcgc gattcataga ttggacataa
    10681 aagatttgtt gaccgctatt aatggcatga aagcgtccag gcataatcgt ttgaattaaa
    10741 gttttgattc cggtagaacg tattaacttg gtgcgttcaa cagcaatcac agggcttgcc
    10801 caaatcatta cgatggcaac cactaccaac acaacagtag ccatggcaaa gctgtgtttc
    10861 agtaattgcc tggggccata accacaagct cgcagaaccg tcatttcgct ttcggcatat
    10921 aagcggccat acgccaataa aagagcgaca taaaacccca ggggcagcaa cagacccatt
    10981 aaatttggca actccaacat caacaacttc atgatcacaa aaccaggaat tgaaccagaa
    11041 gcagcgcgat ttaagtattg tatgaattga ttactcatga aaataaggac taatatcgcg
    11101 gttaatgcga ttaaagtaag aaacacttct ttagtcaaat aacgaaaaat aatcactaca
    11161 ataccctaca gaggacctta tcaattaatt caattctttc agaataacat atgaaagaac
    11221 ccatcatgat aattcaagac agcctattta aaaatagtat tgatttaaaa cagatgatta
    11281 aaacagaatt tttcattcaa atgcttatcc aatttaggta aactagttgt ctttctcaag
    11341 cggtgcagga aaaaatatta tgaattatgg attaactcat accccctcat taaccacgag
    11401 cgaatgtctg gtgttaggcg ttttttcaga tttggcgcta ccagattttg caaatgcaat
    11461 tgataatgaa cagcaaggcc tgattaaaaa actttgccag agagtccctg aaccaggaaa
    11521 tactgtatgg caaactgatg ttgaaggaca tagcctatta atcattcagt gtggcaaaaa
    11581 ggaagagttt agtgctaatt cactccaaaa acgtgtagga gaaattaccg aagcattaat
    11641 taaacagcgt ttctcttctg ccaccgtgtg cttgcctcgg ttaactcagg aatctgctga
    11701 atggcagata gagcaaatga ttgttcaaat cgataattta cgatatcaac ttcttgattt
    11761 taaaacgaaa catgcaaaaa gtcataaatt ggagtcggta atattccatc tgcctggagc
    11821 tactgaaaaa agccttgaaa tggccaaagc cattgttgcc ggagttgaat tttgccgtga
    11881 tttagcgaat atgccggcca atatttgtac tccgacttac cttggtgagc aagccatttt
    11941 attatccaaa caattcgacc aaataagctg tcaagtgatg ggaccagaag aaataaaaga
    12001 aatgggtatg ggcgcattat tagccgttgc ccaaggaagt gaccaacccc caagattgat
    12061 agatgttcat tatcacggta acaaaaactc agcaccggtc attcttgtcg ggaaaggcat
    12121 tacttttgac tcaggaggct tatctataaa gccggccaac gccatggatg aaatgaaata
    12181 cgatatgtct ggcgctgcca gtgtgctggg agttataaaa gcctgtgctt tgctgaaatt
    12241 gccaattaat cttatcggca ttatagccag tgctgaaaat ttgataagtg gctccgctgt
    12301 aaaatcagga gacattgtaa ccaccatgtc aggacaaact gtcgaaatta tcaataccga
    12361 tgcggagggt cgattagtac tagcagacgc tttgacttac gcggagcgat ataatcctga
    12421 ttttgtaatc gatattgcta ctttaacagg agcgataatc gtcgctttgg gcaatatagc
    12481 tactggctat atgactagag atgaacagct ggccaaatca atcgaacgtg ccgctaatga
    12541 aagccaggat aaagtctggc gtatgccact ggatgaggcc tatcaagatg ctttggaaag
    12601 cccattggct gatatgatca acgccggctt tgatcgcagt gcaggaagta ttactgccgc
    12661 ctgtttctta tcacggttta ctgaaaaata tcgttgggcc catttggaca tcgcgggaac
    12721 ggcctggatt tctggaaaga agcgtaatgc aacagggcgt cctgttcctt tattaattca
    12781 attattgcgc catgtcgcca attcgcgttg atttttactt attagccagc agccttccag
    12841 aatctcgctg gttggttgct tgtcgtttaa tagaaaaagc ctataccaaa ggacatacta
    12901 tttttgtttt ttgcgacaat cagcaacaag cagaatacct tgatgaatta ttgtggactt
    12961 tcaaagacga cagttttatt cctcataatt tgcaaggtga aggaccggag cctcctcctc
    13021 ccgtacaaat tggctatcaa aaagaaccca gaggctttac agatatcctg ttgaacttat
    13081 ccaccaatat tccaattttt tatacccgat ttaaaagagt cattgaattg gtgagtaatg
    13141 tagaacatga gaaagaagtg agtcgattgc actataaaga atatagaaac aaaggctgtg
    13201 cattgcacac acactcaatt gaataaatat ttttctgatc gattgatttt taagcttatt
    13261 gtgtatccgc tccctgatgg tcgcgtctct gattataata aagtaaaaca gagccgcgac
    13321 catcagggag cggataaagg ataaagaagc ccggagaatc agcagttaca ttttaacctg
    13381 gtgattttga atgagaccac agcacatcac aacccttttt ttccgcggct tgcaataagt
    13441 gaattaaagg gattgctcgt ttagccaaac tgatatcagg ctcactatct tcatcgtctt
    13501 ctagcagttt attttcgctt tttatttttt ccagcccttg ttgtaaatga tgcaaagcat
    13561 caggtacatt ttcagactta atagcaccgg ggattgtgcc gccatgcccc attaacaaca
    13621 ataattgttt tgccacattt tcaaaataag taatgttctc atatgcatca caactaaaag
    13681 tcacaaacat aactagctcc caactgaaaa ttctatccgt aagtctgtaa taaatactcg
    13741 gcaaccgctc gcaagcccat tgcttcacca ccttctggct ttcctggctt acttactgca
    13801 ttccaggcca tgatatcaaa atgaacccat ggtattgatt ttgtcacaaa tcgttgtaga
    13861 aataagccgg cagtaatagc cccagcatag gacgaagaac tggaattgct catgtctgca
    13921 atattcgaat tcaataactc ttcgtatggt gaaaataaag gcaaccgcca gacggggtct
    13981 gagactttat tagatgctgc tactatatcc tgagccaatt gatcatgatt agaaaacatg
    14041 gctgcaattt cagttccgac agcgacacga gcagcacctg ttaaagtagc aaaatcaatg
    14101 atcaaatctg gtttttcttc acaagccata gccaaagcat ctgccagaat caatcgacct
    14161 tctgcatcag tgttttctat ttctacggtt aa
//
LOCUS       DACTJE010000075        14086 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.075, whole genome shotgun sequence.
ACCESSION   DACTJE010000075 DACTJE010000000
VERSION     DACTJE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14086
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.075"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..411)
                     /locus_tag="I9760_001719"
     CDS             complement(<1..411)
                     /locus_tag="I9760_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043487.1"
                     /translation="MLKESWLLSKSRPRRLSVIAKRFQKVFLESLRYRLVLSIWTGLI
                     LCYVTSFSTQAAPLISVEKIQKLAQSYQGDTRKRFTAWGNLIDSLKKKPVKIQLEKVN
                     SFFNQFNYETDPITGASDDYWKSPVEFIVDGGGDC"
     gene            complement(362..2086)
                     /locus_tag="I9760_001720"
     CDS             complement(362..2086)
                     /locus_tag="I9760_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HAT5043488.1"
                     /translation="MRKALLVIAMLTSSYVAADTLYEAVQYGMIANPDILLNTAKGLS
                     AKQAIDKAKGGLYPSIDVTGGFGRQRSVNPTSAAIDDTPSTTLNIVESAVELRQRLFA
                     GGGIINEVKRNQYLTEAQKWKTQGIAEDLALEITKNYFAVLLHERLYAYSIENLKAHK
                     AVFKMIKERATAGITRTAEVDQANARLALAEANKISALADLQEVKINYAKTVGKWPEN
                     LQVPHVPARKSLPNNLARIIEKGLDNHPTVKSSYADIKEAKAQYDVARAAYYPEVNLV
                     LNSSKNKNLGGLIGPNDSDTVAVRMNYNAFRGGADAARIRETAYQVQEAYETKNRTLL
                     ELKETIRLAWNAYVASALRIQPLKQHVTASRKTRTAYQDEFKVGKRTLLDLLDSQNEY
                     YESQIELARAENDEILSRYRILNGMGCLLKHLNLRLPVNVVNNDVFSSAQTNILLNRK
                     MDEIPYPNNTDNPMVLTHPVKNMEKTRLTKSMVDKNTTYPQQITPKLWYISAGSFRDK
                     NNAVALVKRLNGLGFTAFMSPYKDLLSVFVGPYEYRGHAGNGMERLKELAHVEGVLVT
                     FKEPPKKA"
     gene            2236..3567
                     /locus_tag="I9760_001721"
     CDS             2236..3567
                     /locus_tag="I9760_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAT5043489.1"
                     /translation="MSSQLPILTVSQLNRQVKGFLENEIGLVHVEGEISNLSKPSSGH
                     YYFTLKDSTAQIRCAFFKNRHSNSLLRNFNDGQQIVATGKLSLYEARGEYQLIVEEIV
                     KAGMGILYQRFEELKIKLASEGLFNPERKKPLPRIPETIGIITSPTGAAIQDILSTLA
                     RRFPIARIIIYPSEVQGQTAPQQLVKALKLANAHKRCQVLILARGGGSIEDLWAFNDE
                     YLARQIAISEIPVVSGIGHETDFTIADFVADYRAETPTAAATAVTPNCIELFNILDTA
                     IYRLHDAIIRLVKGLQLKLNHLIDKIASPRQTISTYWQTLDYLERQLISAMTQFINLN
                     INKVNIFSTQLQANNPKTQIERTKTQLRQLIMQLNQEIRIQVNQLKNQLSTNLSTLHA
                     VSPLATLDRGYAIVSKNQRILFAAQQAQIGDTIDVRLAKGSLACEVTQIKD"
     gene            3576..4478
                     /locus_tag="I9760_001722"
     CDS             3576..4478
                     /locus_tag="I9760_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HAT5043490.1"
                     /translation="MKNLLHSLACLLILISSAKLSYGLALPENHSVNGGLTIIPIDFN
                     QKPEAYFEGKRIPVLPSTQPRQWLLIVAIPLKNTNSVQYINVTKPIKASIPFHVSEKF
                     YSTQFLNIKDISKVDPQPEDLIRIEKETKKLTQIFANYSNTNPFEKQFTAPLRGPITS
                     LFGLKRVYNKQPRDPHSGLDIAADEGKPVYAVNQGVVADTGDYFFTGNTVILDHGMGV
                     FSVYAHLSKILVKTGETIKQGQELGLVGMTGRVTGPHLHWTMVVNQTLVEPLLFVPFR
                     KITVTPVIPDKKSNKSTQDATAKS"
     gene            4475..5074
                     /locus_tag="I9760_001723"
     CDS             4475..5074
                     /locus_tag="I9760_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAT5043491.1"
                     /translation="MINELTTSLNVIVTQTQNNLKILGVILLIPWSIFFISHFISNKI
                     LLLGIIPRHILGLPGILLAPLLHANFNHLFFNSIPLLVLSNFILINGVNYYLIVTVMI
                     TLFSGTLIWCFAKRGIHIGASGLITGYWGFLVYNIYQTGTLTTIILGLLSLYYFAGIF
                     LGIFPGEKGVSWQGHLFGLLAGFLTSYLLEIYPQILIYY"
     gene            5244..5579
                     /locus_tag="I9760_001724"
     CDS             5244..5579
                     /locus_tag="I9760_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurogenic locus notch"
                     /protein_id="HAT5043492.1"
                     /translation="MNILKPKAFLLASVFVLSISPAFAADGCCSKMGGINYCDSSAGR
                     LVCNNGFYSTCYCTRHAVMDLQFLMGCCLWHGGVYPQLNSSGLVVCNDGYVSEECSLQ
                     KPVEQISVY"
     gene            complement(5580..6830)
                     /locus_tag="I9760_001725"
     CDS             complement(5580..6830)
                     /locus_tag="I9760_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAT5043493.1"
                     /translation="MRLTIMDTKGILLAPFFFISFMFFIPISPTIKSICFICSLIAVL
                     ITPQYRKHIVYAYNTLWGRAAFCLFLFVVIASLWSPAPYSMQWMVIGKYCKLIYLPIL
                     ATGFINPKVRNWSINSYLAAIFITCVISILKAKNIIASEDPGEVFYNHIITGFMVALA
                     AYLAGLLAFQSSGWLRLIYIFMILLTSYQVLFINTGRTGYLVYFILMLLLLIQKLNFK
                     QALAGIVLFSGIIALAYHHSPVMQTRIYDLIQDIKLLKQHNKNTSIGYRMQFHHYAKQ
                     LMLKHPFIGIGTGGFKYSYSQDVPVPSWGKELTDPHSQYWMTLAEQGLIGLVLLIIFL
                     ASLFITSFQLTSSRPILLGVLFSFCIGSISDTILCYSTAGYLLVVMSALCFGELIEKR
                     RVKDAKEDHPALLSAHAPAAISQT"
     gene            complement(6833..7612)
                     /locus_tag="I9760_001726"
     CDS             complement(6833..7612)
                     /locus_tag="I9760_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT5043494.1"
                     /translation="MLSVIIITKNEEANIRRCLESVHFADEIIVLDSGSTDNTLAIAR
                     EYTDKVFSTDWQGYGIQKDRALQKAQGDWVLNLDADESVSSELRQEILQAISSDTADA
                     FRIPIQMIFYNQVLKYSGSPKRHIRLFKRENASYSKDIVHEKVLIPVNARVGKLKKPI
                     WHHSFSDVHHVLYKLNKYSSYSAKIRIESNEKVGLVKTFFSALWMFVRCLFLQKGFLD
                     GRAGFLFAVFGAQGAFYRGVKQIYKDRDINKLPSVNHIKEE"
     gene            complement(7836..10103)
                     /gene="clpA"
                     /locus_tag="I9760_001727"
     CDS             complement(7836..10103)
                     /gene="clpA"
                     /locus_tag="I9760_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAT5043495.1"
                     /translation="MLNKELEFTLNLAFKEAKEKRHEFMTVEHLLLSLLDNPAAGNVL
                     QACDANIEALRRDLIEFIDETTPRIPEDELDRETQPTLGFQRVLQRAVFHVQSAGKTE
                     VTGANVLAAIFSEQESQAVYFLRRENITRLDVINYISHGVSKYQNNDMHENMNSSMDE
                     DMMTPEGNESPLDSYCTNLNRRAKQGKIDPLIGRHEEIQRTIQVLCRRRKNNPLLVGE
                     AGVGKTAIAEGLARRIVDGEIPDSIRGCVVYSLDLGALLAGTKYRGDFEKRLKAVLKQ
                     LGQQEGAILFIDEIHTIIGAGAASGGVMDASNLVKPLLANGELKCIGSTTYQEYRGIF
                     EKDRALARRFQKIDISEPTIDETFEILKGLKGKLEAHHGVKFSIASLKAAAELSAKYI
                     NDRFLPDKAIDVVDEAGAYQNLLTANKRKKIISVTEIESVVAKIARIPIKKVSARDKD
                     TLRNLERDLKLLVYGQDQAITALASAIKLGRSGLREPQKPVGCFLFAGPTGVGKTEVT
                     RQLANVLGIELLRFDMSEYMEKHTVSRLIGAPPGYVGYDQGGLLTEAVTKNPHAVLLL
                     DEIEKAHPDVFNLLLQIMDHGTLTDTNGRQADFRHIILVMTSNAGASELVRNSIGFST
                     QDNTNDGLEVIKKQFSPEFRNRLDAIINFAPLDTQTIGLVVDKFIMELDEQLSHKGVT
                     FTVDKSAREWLMEHGYDKAMGARPMARLIQENIKKPLADELLFGKLMHGGHVTLRVKD
                     GKLHFDSHDHREGVC"
     gene            complement(10135..10470)
                     /gene="clpS"
                     /locus_tag="I9760_001728"
     CDS             complement(10135..10470)
                     /gene="clpS"
                     /locus_tag="I9760_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAT5043496.1"
                     /translation="MSRQNLEEIIQTGIADTELSTEISTAIKRPRKYKVLLLNDDYTP
                     MDFVVEVLKHFFHLNEEVAIQVMLQVHFQGKGVCGVFTRDIAETKVALVNEYARMNQH
                     PLLSSMEPE"
     gene            complement(10872..12128)
                     /gene="icd"
                     /locus_tag="I9760_001729"
     CDS             complement(10872..12128)
                     /gene="icd"
                     /locus_tag="I9760_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAT5043497.1"
                     /translation="MTYDKIKVPAQGEAITVAADHSLHVPDNPIIPFIEGDGIGVDVT
                     PPMIRVVDAAVQKAYGNKRKISWMEVYAGEKATKVYGGDQWLPKETLDAMKKYVVSIK
                     GPLTTPVGGGIRSLNVAIRQDMDLYVCLRPIRYFNGVPSPVREPWKTDMVIFRENSED
                     IYAGIEWQADTPEAKKVIQFLTQEMGVKKIRFPEHCGIGVKPVSREGTTRLVKAAIQY
                     AIDNDRSTVTLVHKGNIMKFTEGAFKDWGYQVARDSFGAKEYQGGPWMEFKNPKTGKQ
                     IIINDVIADAFLQQILLRPEDYSVIATLNLNGDYISDALAAQVGGIGIAPGANISDQM
                     AVFEATHGTAPKYAGQNKVNPGSIILSAEMMLRHMGWYEAADLIIRGMEGAIEAKTVT
                     YDFERGMQGATLVSSSGFADAMIKHM"
     gene            complement(12238..12936)
                     /locus_tag="I9760_001730"
     CDS             complement(12238..12936)
                     /locus_tag="I9760_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HAT5043498.1"
                     /translation="MKWVLVVMAIIAQTSCAVNHRVMVGDEPVIIQQTKGQGKTFVHV
                     HHNEQTALKAAKAVIRKEGGSLITLIHSGGRNIVFHLHNQRYEFDPNRIFTDKGIKKT
                     LTQYSYYTPEAHQEVKKLADRIKALLPEGKVIAVHNNSSYSLKDYLPGHELAKDAKAL
                     YMNPDNYFRNFYLVTKLSDYLRLKMQGFNGVLQKPSATDDGSLSVYLAKRDYINVEAG
                     YDQLAEQIKMLLHA"
     gene            13197..13799
                     /locus_tag="I9760_001731"
     CDS             13197..13799
                     /locus_tag="I9760_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAT5043499.1"
                     /translation="MPQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRLDKQ
                     SEGLLILTDDGKLQHRLTHPKFEKKKHYWVQVEGVPTDKDLQPLRKGLVIKDITFLPA
                     EAKLISEPKIWPRIPPIRERKHIPTTWLGIILREGKNHQIRKMTAAIGFPTLRLIRYQ
                     IGDWHLDQLQPGEYKLINLTNDKLNELTRKSNPRLESRAK"
     gene            complement(13735..>14086)
                     /gene="mreD"
                     /locus_tag="I9760_001732"
     CDS             complement(13735..>14086)
                     /gene="mreD"
                     /locus_tag="I9760_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAT5043500.1"
                     /translation="YYLPGKFKLTALLIVGLLMDVLLSTVIGEHSFALLLVTWIASTK
                     SRRVQFFSLIQQVCLVGFFCFLYQSIIIFIDALLGFNYNLIMPVTSAILGMIIWPWIR
                     VLGYSSLLTRSVYR"
BASE COUNT         4360 a         2975 c         2397 g         4354 t
ORIGIN      
        1 acaatcacct cccccatcaa caataaattc tactggtgat ttccaataat catcacttgc
       61 accagttatt gggtcagttt cataattaaa ttgattaaag aaagagttta ctttttccag
      121 ttgtattttt actggctttt ttttaagtga gtcaattaaa ttcccccatg cggtaaaccg
      181 tttccgggtg tccccctggt aggattgcgc caatttctga attttttcca cactgattaa
      241 aggcgcggct tgagtggaaa agcttgttac atagcaaagt atcaaaccag tccaaatgga
      301 taaaaccaat ctataacgaa gagattccaa aaaaaccttc tgaaatctct tagctataac
      361 actaagcctt cttgggcggc tctttgaaag taaccaggac tccttcaaca tgagccaatt
      421 cctttagcct ttccattcca ttacccgcat gacctctgta ttcataagga ccaacaaaaa
      481 cggacagaag atctttataa gggctcatga aggcagtaaa gcctaatcca tttaaccgtt
      541 taaccaaagc tacggcatta tttttatctc tgaaagagcc tgctgagata taccataact
      601 tgggcgttat ttgctgagga taagtagtgt ttttgtctac catcgattta gttaaacgag
      661 ttttctccat gttcttaaca ggatgagtga gaaccatagg attatccgta ttattgggat
      721 aagggatctc atccattttc ctgttcaata aaatattggt ctgagccgac gagaatacat
      781 cattattcac cacatttacc ggcaacctca aattcaaatg ttttaataaa caacccattc
      841 cattcaggat tcgatagcgg gaaagtatct cgtcattttc cgcgcgggcc aattcaattt
      901 gtgattcata atattcattt tgagaatcga gtaaatcaag gagcgtcctt ttacccacct
      961 taaattcatc ttgataggcc gtacgtgttt ttctcgaagc ggtaacgtgt tgcttcaatg
     1021 gttgaatgcg caaggcagag gcaacataag cattccaggc taacctgata gtctctttta
     1081 attccagcaa tgtgcgattt tttgtttcat aagcttcctg gacttgataa gctgtttctc
     1141 gtatacgagc ggcatcagca ccaccacgaa atgcattgta attcattctt acagccaccg
     1201 tgtcggagtc attaggacct atcagaccac ccaagttctt gttttttgag ctattcaaaa
     1261 ccagattaac ctcagggtaa taggctgctc tggcgacatc gtactgagcc tttgcttctt
     1321 taatatcagc gtaacttgat ttcactgttg ggtgattatc taatcctttt tctataatcc
     1381 tggctaaatt attaggcaaa ctttttcttg ctggaacatg aggcacttgg agattctctg
     1441 gccatttacc tactgttttt gcataattga tcttcacctc ttgcaaatca gccaatgcac
     1501 ttattttatt agcctcggca agagccagtc ttgcattagc ctgatcgact tctgccgttc
     1561 tggtaatccc ggcggtagcc cgttctttta tcatcttaaa aaccgcttta tgtgctttaa
     1621 gattttcgat ggagtaagca tacaaacgtt catgcagtaa tacagcgaaa taatttttgg
     1681 taatttccaa tgccaaatct tcagcaatac cctgtgtttt ccacttttgc gcctcagtaa
     1741 ggtattggtt tcgcttcact tcattaatta tccctccacc agcaaacaat ctctgcctta
     1801 actccacggc cgattccact atattgagag tagtactggg agtatcatcg atcgctgcag
     1861 atgtcgggtt aacactcctt tgtcgtccaa acccaccagt tacatcgata gaaggatata
     1921 aaccaccctt tgctttatca attgcctgtt tcgctgacaa accttttgca gtattaagta
     1981 gtatatccgg gttagcgatc atgccatatt gtactgcttc ataaagagta tctgcagcaa
     2041 cgtaggaaga agtaagcatt gcaattacca acagagcctt tctcattcct cattccttat
     2101 tattgttatt ttgttaatta tctatatagt aacgtataac cttaaatgaa ataacatctc
     2161 actacaatat aattttattt ctttaattag ttattgttgt taacaacctt ttgatttgat
     2221 gggattttta ccaccatgag tagtcaattg ccaatactga ccgtgagtca gctgaataga
     2281 caagtgaaag gttttctcga gaatgaaata ggccttgtgc atgtcgaagg cgaaatctct
     2341 aacttaagca aaccttcttc aggacattac tatttcaccc tgaaagacag cacagcgcaa
     2401 attcgttgcg ctttttttaa aaatcgacat tccaattctc tgcttagaaa ttttaatgat
     2461 ggtcaacaaa ttgttgccac tggcaaatta agtctgtatg aagcccgggg agaatatcag
     2521 cttattgtcg aagagatagt taaagcagga atggggattc tctatcaacg atttgaagag
     2581 ttaaaaatca aactggccag cgaaggctta ttcaatcctg agagaaaaaa accgctgccc
     2641 agaatacccg aaacgattgg catcattaca tcacccaccg gagcagctat ccaggacatt
     2701 ctctcaactc tggcccgtcg attcccaata gccagaatta ttatttatcc aagtgaagtt
     2761 caggggcaaa ccgcccctca acaactagtc aaggccttaa aactggctaa tgcccataaa
     2821 cgatgccagg ttctcatcct ggcgcgcggc ggtggcagca ttgaagactt gtgggccttt
     2881 aatgatgaat accttgctcg acaaattgca atcagcgaaa ttcctgttgt ttccggtatt
     2941 ggtcatgaaa cagatttcac aattgctgat tttgtagcag attatcgtgc agaaacgcct
     3001 actgctgccg ctacggctgt tactccaaac tgcatcgaat tattcaatat tcttgatacg
     3061 gctatttatc gattgcatga tgccatcatc agactagtta aagggttgca attaaagctc
     3121 aatcatttga tagataaaat tgcctcaccc aggcaaacta tctcaacata ttggcaaacc
     3181 ctggattatc tggagagaca gttaatatct gctatgactc aatttattaa cttaaatatt
     3241 aataaggtga atattttttc aacacaattg caagctaaca atcccaaaac acaaattgaa
     3301 cgaactaaaa cccaactacg acaattaata atgcaattaa accaagagat ccggatacaa
     3361 gtaaaccaat taaaaaatca actatccact aatctttcca ctttgcatgc tgtaagcccc
     3421 ttggctacac tagatagagg ttatgctatt gtgagtaaaa accaaagaat actctttgct
     3481 gcccaacagg cacaaattgg agatactatc gatgtccgtt tagcaaaagg aagtcttgct
     3541 tgtgaggtta ctcaaattaa ggattgacta aaatcatgaa gaatctatta cattcacttg
     3601 cctgtctatt gatattaata agctcagcaa aactatccta tggtctggct ctaccagaaa
     3661 atcattctgt caatggggga ctgactatta ttccaattga tttcaatcag aagcctgaag
     3721 cctatttcga ggggaaacgc atcccggttc ttcccagtac ccaaccccgg caatggttat
     3781 taatcgttgc aatcccgctt aaaaatacca actctgttca atacatcaac gtgacaaaac
     3841 ccatcaaagc atctattccc tttcatgtta gtgaaaaatt ttacagtacc caatttttaa
     3901 atataaaaga tatcagtaaa gtggatcctc aacccgaaga tttaatccgt attgaaaaag
     3961 aaaccaaaaa attaacccaa atttttgcta attactccaa taccaatcca tttgagaaac
     4021 aattcacagc accactgaga ggacctataa ccagtttatt tggcctgaag agagtttata
     4081 ataaacagcc aagagatccg cattctggat tggacatcgc cgctgacgag gggaaacctg
     4141 tgtatgctgt aaatcaggga gttgtagcag ataccggtga ttattttttc acaggcaata
     4201 cggttatcct tgatcatggc atgggagtat tctcggttta tgctcatctt agcaaaattc
     4261 tggttaaaac aggagaaaca atcaaacaag gccaagagct tggattagtt ggtatgacag
     4321 gaagagtaac aggcccccat ctccactgga ctatggtagt taaccaaact ttggttgagc
     4381 ctttactttt tgtgccgttt cgcaaaatca cagttacacc agttatacca gataaaaaat
     4441 caaacaaatc aacacaagat gcaacggcaa aatcatgatt aacgaattaa cgaccagttt
     4501 gaatgtgatt gtcacccaga cacaaaataa cctgaaaatt ttgggagtaa tactactaat
     4561 tccctggtca atatttttta ttagtcattt tataagtaat aaaattttgc ttcttggcat
     4621 tatcccaagg catatattgg gcctcccggg gatactatta gcacctttac ttcacgctaa
     4681 ttttaatcac ttatttttta actctatccc tttactggtt ttaagtaatt ttattctgat
     4741 taatggagtt aactattatt tgattgtcac agtgatgata actcttttta gcgggacact
     4801 gatttggtgt tttgctaaac gaggtataca cattggcgcc agtggtttaa tcacaggcta
     4861 ctggggattc ctcgtgtata acatttatca aacaggtacc ttgaccacta ttattcttgg
     4921 tctgcttagc ctgtattatt ttgctggtat ttttctggga atttttccag gtgaaaaagg
     4981 cgtatcctgg caaggccact tgtttgggct attagcaggt tttctcacca gctatttatt
     5041 agaaatctat cctcagattc tcatttatta ttagtcatta actcttcaca atagctagcc
     5101 catgaaacca atttatcatt tcactttgaa cttttttgtc ctaataaatt cagtcttaaa
     5161 ctgcaattaa atcatcatat ttcactaata atccatctaa ctctatgcta ttcttaaaaa
     5221 taaaaaccaa tcagagaacc acaatgaaca tattgaaacc aaaagcattt ttactagcca
     5281 gtgtgtttgt tttatcaata tcccctgctt tcgctgctga tggttgttgc agtaaaatgg
     5341 ggggtataaa ttattgcgat tcttctgctg gtcgtcttgt ttgtaacaac ggtttctatt
     5401 caacttgcta ctgcacacgt catgccgtaa tggatttaca atttcttatg ggatgttgct
     5461 tatggcatgg cggggtctat ccacaattaa actcatcggg tcttgtggtt tgcaatgacg
     5521 gatatgtttc ggaagaatgt tcattgcaaa aacctgttga gcaaatttct gtttattgat
     5581 taggtctgac ttattgctgc tggagcatgg gctgataaaa gagcaggatg gtcttctttt
     5641 gcgtccttga ctctacgctt ctcaattaac tcaccaaaac ataatgcact catcactacc
     5701 aacaaataac ctgcagtcga atagcataaa atcgtgtcgg aaatggagcc tatacaaaaa
     5761 gaaaataaaa cacctaaaag aataggtctg gagctagtca attgaaatga tgtaataaac
     5821 aaactcgcta aaaatattat caaaagtacc agaccaatta aaccttgctc agccaaagtc
     5881 atccaatact gactgtgtgg atctgtcaac tctttacccc aacttggaac aggtacatct
     5941 tgtgaataac tatatttaaa tccacctgtg cctattccaa taaaaggatg tttcaacatc
     6001 aattgcttgg catagtgatg aaactgcatt ctatagccta tcgatgtatt tttattatgt
     6061 tgttttaata gcttgatatc ctgaattaaa tcataaattc tggtttgcat tacagggcta
     6121 tgatgatatg ccaaagcaat gatgccgctg aagagaacaa tccctgctaa agcctgtttg
     6181 aagtttaatt tttgtatcaa aagcaataac atcaaaataa agtagaccaa ataacccgtt
     6241 cttccagtat ttataaaaag cacttggtaa ctggttagta atatcatgaa gatataaata
     6301 agtcgcaacc agcccgaact ttgaaaagcc agcaatcctg ctaaataggc tgccaatgcc
     6361 accataaaac cagtaataat atgattgtag aaaacctcgc ccggatcttc tgacgcaata
     6421 atatttttag cttttaagat ggaaataaca caagtaataa aaatagcagc caaatagcta
     6481 ttgatactcc agtttcttac tttgggatta ataaaaccag tagctaaaat gggaagataa
     6541 attaacttac aatatttacc aataaccatc cattgcattg aataaggcgc aggactccat
     6601 aaacttgcaa taacgacaaa gagaaacaaa caaaatgcag cgcgtcccca caaagtatta
     6661 taagcataaa caatatgttt tctgtattgt ggagtaatca atacggcaat taaactgcaa
     6721 atgaaacaaa tagactttat cgtgggactg atcggaataa aaaacataaa tgaaataaag
     6781 aaaaaaggag ctaataaaat acccttggta tccattatgg ttaatcgcac gattattcct
     6841 cttttatatg atttacactg ggtaatttat tgatatccct gtctttatat atttgtttga
     6901 ccccgcgata aaaagcgcct tgcgccccaa atactgcaaa caaaaaacct gccctgccgt
     6961 ctaaaaatcc cttctgtaaa aaaaggcatc tgacaaacat ccatagggcg ctaaaaaaag
     7021 tttttactaa tccaactttt tcattggatt ctatacggat tttcgcacta taagaagaat
     7081 atttgttaag tttgtatagt acatgatgta catcgctaaa agaatgatgc caaatcggtt
     7141 tttttagttt tcccactctc gcattaacag gaatcaatac tttttcatga acgatgtctt
     7201 tgctataaga agcattctct cttttaaata atcgtatatg ccttttagga cttcctgaat
     7261 attttaaaac ttgattataa aaaatcattt gtataggaat acgaaatgca tctgctgtat
     7321 ccgatgaaat ggcctgtagt atttcctgtc gtaattccga gcttactgat tcatcagcat
     7381 caagatttaa aacccaatcc ccttgagcct tctgtaaggc cctatccttt tggataccat
     7441 agccttgcca atctgttgaa aatactttat ccgtatactc cctggctata gctaatgtat
     7501 tatctgtgct gccagagtcc aatacaatta tttcatctgc aaaatgaacg gattccaagc
     7561 accgccttat gttggcttct tcatttttag tgataatgat tacggataac atgactttat
     7621 atcaatttaa aataaatatt atatcgataa ttaagccgga ttgtaacaag gtaaaaagga
     7681 ataatattcg tttagcggtc acaaaaggat atcttgataa caattaccag ttatgcgact
     7741 aacaggttaa cgacaattac tatctcagga ttcttgtgat tttgtctgtg cataacaaaa
     7801 aatttgtcat gcacagaact gaaaagggtt cccgtttaac aaacaccttc tctatgatca
     7861 tgactgtcaa aatgcagttt accgtctttc actcgcaaag tgacatgacc gccatgcatt
     7921 aatttaccaa acaacagttc atcagcaaga ggctttttga tgttttcctg aatcaatctg
     7981 gccataggtc ttgctcccat ggctttatca taaccatgct ccatcagcca ttccctggct
     8041 gacttatcaa ctgtaaaagt aacgccttta tgacttaatt gttcatccaa ctccataatg
     8101 aatttatcaa cgactagtcc tatagtctgt gtatctaacg gagcaaaatt gataatagca
     8161 tccaaacggt tcctaaactc aggactgaat tgctttttaa ttacttccaa cccatcattg
     8221 gtattgtctt gcgttgaaaa acctatggag ttacgaacaa gttcactggc tcctgcgtta
     8281 ctggtcatta caagtatgat atgtctgaaa tccgcttgac gaccgttagt atcagttaaa
     8341 gtgccatgat ccattatctg caacaataaa ttaaaaacat caggatgtgc tttttctatt
     8401 tcatcaagta gcagaacagc atgtggattc ttagtcacgg cttcagtcaa taaaccacct
     8461 tgatcgtatc ctacatagcc ggggggtgct ccaatcaaac gtgaaacagt gtgtttttcc
     8521 atgtactctg acatatcaaa acgcagcaac tctatgccta acacattagc cagttgcctt
     8581 gttacctctg tctttcctac accagttggc ccggcaaaca agaaacaacc tactggcttc
     8641 tgaggttctc taagtccgga tctacccagt ttgattgctg aagccaaggc agtaattgcc
     8701 tggtcttgcc catagactaa taattttaaa tcacgctcca ggttacgcag agtatcttta
     8761 tcgcgcgcag ataccttttt aattgggata cgagcaattt ttgcgacaac actctctatt
     8821 tcagtcacgc taataatttt tttacgttta tttgctgtca gtaaattctg ataagcacct
     8881 gcttcatcca ccacatcaat cgctttgtca ggcaaaaacc gatcattgat gtattttgca
     8941 gataattctg ccgctgcttt taatgaagca attgaaaatt ttactccatg gtgagcttcc
     9001 aacttgcctt ttaatccttt taatatttca aaagtttcat cgattgttgg ttctgaaata
     9061 tcaatttttt ggaatcgtct tgccaaggcc ctgtcttttt caaatattcc acgatattcc
     9121 tgataagtgg ttgagccgat gcatttcaat tcaccatttg ccaataatgg cttaaccagg
     9181 ttggaagcat ccatgacccc tccagatgca gcacctgcgc caataatggt gtgaatttca
     9241 tctatgaata aaatggctcc ttcttgctga cccaactgtt tcagcacagc tttcaatcgt
     9301 ttttcaaaat cccctctgta tttcgttcct gccaataacg cgcccaaatc aagagagtag
     9361 actacgcagc ctcttatgga atctgggatt tcaccatcca ctatgcgtct ggccaaacct
     9421 tctgcgatag cagttttacc tacacctgcc tcgccaacta ataaaggatt atttttacgg
     9481 cgtctgcaaa gtacttgtat agttctttgt atctcttcat gccgtcctat caacggatct
     9541 atcttgccct gttttgcacg tctgttgaga ttggtgcaat aactatcgag cggagattca
     9601 ttaccctcag gtgtcatcat gtcttcgtcc atagatgaat tcatgttttc atgcatatca
     9661 ttgttttgat attttgacac tccatgagaa atataattaa tcacatcaag gcgagtaata
     9721 ttttcacgtc gcaaaaaata tacagcctga ctttcttgct cactaaaaat tgcggctaat
     9781 acattagctc cagtgacttc cgttttgcct gctgactgca catgaaatac tgcacgctgt
     9841 aaaacccttt gaaaacctaa tgttggttga gtttccctgt ctagttcatc ctcaggaatt
     9901 cttggggttg tttcatcaat aaattcgatt aaatcacgac gtaatgcctc gatgttagca
     9961 tcacaagcct gtaatacatt tcctgctgct ggattatcaa gcaaagacaa cagcaaatgt
    10021 tcaacagtca tgaattcatg acgcttttct tttgcttcct tgaaagctag attcaaggtg
    10081 aattctagtt ctttatttaa cattgtcatt gctccttacc aacccaccac cagtttactc
    10141 cggttccata ctagataata atggatgttg attcattctg gcgtattcat ttaccagggc
    10201 gacttttgtt tctgctatgt ctcttgtaaa cactccacaa acccccttcc cttgaaaatg
    10261 aacctgcaac atcacctgaa tcgcaacctc ctcatttaaa tgaaaaaagt gcttcagtac
    10321 ctctaccaca aaatccattg gcgtatagtc atcattgagc aataaaacct tatactttct
    10381 tggccgttta atagctgtgc taatttctgt gctaagctct gtatcagcta tccctgtttg
    10441 tattatttcc tctaaatttt gcctgctcat aactaacaac ctcttactat ttttatagac
    10501 tccctacacg catttggcca gtaaatttag ctaattttaa tgaaaaattt agtcattgaa
    10561 ataatcaata gcatagtgat tagatatgat tcactcaaat gttgtactat ttgttattga
    10621 ccaccaggtt ttactcaaga gattaagccg cctctaatca gtagagtaac atcataatta
    10681 gcatggataa agttcctgct caattttcat gaactcgtaa gatgtataaa cctgatagtc
    10741 aaagatacta ccaataatct tgaaaggata atcccagtct ttcatgaaca acgcattggc
    10801 actgagtaaa gctataagca ctatattgtc aatacgtgtt gttcagaaag accatgtgat
    10861 aactccccgg tttacatatg cttaatcatg gcatctgcaa aaccagagct gctgactaac
    10921 gttgctcctt gcataccacg ttcaaaatca taagttacgg ttttggcttc tatcgcgcct
    10981 tccatacctc tgataatcaa atcagccgct tcataccacc ccatatgacg taacatcatt
    11041 tcggcagaaa gaataataga accggggttc actttattct ggccagcgta tttaggcgct
    11101 gttccatgag ttgcttcaaa cacagccatt tgatcactaa tgttcgctcc gggagctata
    11161 cctattccgc caacctgagc agccaaagca tcagaaatat aatcaccatt caaatttaat
    11221 gtagcaatga cactatagtc ttctggtctt aacaggattt gttgcaaaaa ggcatcagcg
    11281 atcacatcat taatgataat ctgcttaccg gttttaggat ttttaaattc catccatggc
    11341 ccaccttgat attcttttgc tccaaaagaa tcgcgcgcaa cctgatatcc ccaatcttta
    11401 aaagcccctt cagtaaactt catgatattg cctttatgta ccagagtcac tgtactgcgg
    11461 tcattatcaa ttgcatactg aattgccgct ttgacaagtc gagtggtacc ttctctggaa
    11521 acaggcttta caccgatacc acaatgctca ggaaaacgaa tcttcttaac acccatttcc
    11581 tgagttaaaa attgaatcac ttttttagct tccggggtat ctgcctgcca ttcaataccg
    11641 gcataaatat cttcagaatt ttccctgaaa ataaccatat cggttttcca tggttcccga
    11701 actgggcttg gaacaccatt aaaatagcga attggacgta aacaaacata gagatccatg
    11761 tcttggcgaa tagcgacatt caatgaacgt ataccgccac caacaggggt agttaaagga
    11821 cctttaattg aaaccacata ttttttcatg gcatccagag tctctttggg aagccattga
    11881 tcgccaccat aaaccttagt agccttttcc ccggcataaa cctccatcca ggaaattttc
    11941 ctcttgttgc catatgcttt ttgaactgca gcatcaacaa cacggatcat cggaggtgtt
    12001 acatcaacac cgataccatc accctcaata aaaggaataa ttggattatc aggaacatga
    12061 agtgaatggt cagcagcaac agtaatagct tcgccttggg caggcacttt gattttatcg
    12121 tatgtcattg acgactccat aaatttagag aaattaatga tagcatgtga taatcgggtt
    12181 tcccaaatat caattacgac tattaattat ttttagctaa attagatcag attggaacta
    12241 ggcatgaagc aacatcttaa tttgttctgc caactgatca taaccggcct cgacattaat
    12301 ataatcacgt ttagcgagat agactgataa agatccatca tctgtagccg aaggtttttg
    12361 taaaacacca ttaaaacctt gcatttttaa acgcagataa tcacttaatt ttgtgaccag
    12421 ataaaaattc ctaaaataat tgtcaggatt catgtacaaa gcttttgcat ccttggcaag
    12481 ttcatgtcct ggcaaataat cttttaagga ataggaggag ttattatgaa cagcgattac
    12541 tttaccctct ggtagtaagg ccttaattct gtctgcaagt ttctttacct cttgatgcgc
    12601 ttcaggagta taataactgt attgagtcaa agtttttttg atccctttgt cggtaaaaat
    12661 gcggttggga tcaaattcat agcgttggtt atgtaaatgg aatactatat ttcgaccgcc
    12721 ggaatggata agtgtaatga gactcccacc ctcttttcta atgacagctt tagcagcttt
    12781 taatgctgtt tgctcattgt gatgaacatg tacaaaagtc ttaccttgac ctttggtttg
    12841 ttgaattatt acaggttcat ccccaaccat aactctgtga ttcactgcac aacttgtttg
    12901 agcgataatt gccataacaa ccaataccca tttcatgaat aagagtcacc ataacgaata
    12961 aaattgcgta attgtatagt gattggccat aaaaaatcaa tcttggctta agttccaagc
    13021 gggacaaaat catattttaa ttaattaatg cacctcatat ctcattgcgt gaatgacaaa
    13081 atataacaat tatttacgct gtttattgtg cttaaaggcg tgatctggcc ctgaagtggt
    13141 tgctgacaat aaccaaaaaa tttatcatta atttccacaa tcaacgcaat cataaaatgc
    13201 cgcaattaat actttttaat aaaccttatg gagtcttgac tcaatttact ggtgaattac
    13261 ccgaacaaac tctctcggca tttattaaat ttccgggttt ttatgcggct ggacgtctgg
    13321 ataaacaaag tgagggctta cttatcttaa ctgatgatgg aaaactacaa caccggttaa
    13381 cccatcctaa atttgagaag aagaaacatt actgggttca agtagagggg gtccctacag
    13441 acaaagattt acaaccttta agaaaaggcc tggtaatcaa agacattact tttttacccg
    13501 ccgaagccaa gttgatttca gagccaaaaa tctggccacg aattccgccc ataagagaaa
    13561 ggaaacacat accaactacc tggctgggaa ttattttaag agaaggaaaa aatcatcaaa
    13621 taagaaaaat gacagccgct attggatttc ctactttacg tttaattaga taccagattg
    13681 gtgattggca cttagatcaa ctccagcccg gggaatataa attaatcaac cttactaacg
    13741 ataaactgaa cgagttaaca aggaagagta acccaagact cgaatccagg gccaaataat
    13801 catacctaaa atggcacttg tcacgggcat tatcaggtta taattaaaac ctaacagagc
    13861 atcaataaat ataataattg attgatatag aaaacaaaaa aaaccaacca aacaaacctg
    13921 ttgaattagt gagaaaaatt gaactcgtct cgatttggtt gacgctatcc aggttaccaa
    13981 tagcaatgcg aatgaatgtt caccgatcac ggttgataag agtacatcca ttaataagcc
    14041 aactatcaac agtgcagtca gtttaaattt acctggtaaa taatat
//
LOCUS       DACTJE010000076        13947 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.076, whole genome shotgun sequence.
ACCESSION   DACTJE010000076 DACTJE010000000
VERSION     DACTJE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13947
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.076"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1091
                     /gene="lem8"
                     /locus_tag="I9760_001733"
     CDS             <1..1091
                     /gene="lem8"
                     /locus_tag="I9760_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem8"
                     /protein_id="HAT5043501.1"
                     /translation="LLMKLKVSHIPNPVVTPNLLQDVSLLQLEGEIEKQRMIAIIDGQ
                     EPLARALKYLSIGCTTTAARTKELQKTIVQHGTNLQAYRHQEKCRELGGRIVLHCSQA
                     MLPGMGLKSGMCHGLARHWANDVIKKDRFMGFRGNQEVSIQPTKATDKILQAIPSFND
                     FVRLNPKIYETQQSQKEHIFLEYEDTVGSEPEKQFEEFTDRIMSELDKYGKSATFVSY
                     ANSSSGHVVAIHKRTKPTPQGFIIDYFDANAGWMQFKDDKSFQEFLTYYLNDRHAKEK
                     LKSIAFETLCYPSSYTHMLDKLCEKAPQPTPQRQRAQSLSAETERNTPQIKSQISEKT
                     EKTEKTEKTEKTEKTENEQSRNTRGFPI"
     gene            complement(1124..1444)
                     /locus_tag="I9760_001734"
     CDS             complement(1124..1444)
                     /locus_tag="I9760_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043502.1"
                     /translation="MKRMIGFMVSVMLTFAVNAADSQVNTHKHQTDKGKVTPGMSIQR
                     NTEQSTAITADAASSNTTEKVTSQQMKEHKKSVKEANYNAQKKEEEAKTATDTQKQEA
                     DSPS"
     gene            complement(1494..2093)
                     /gene="ruvA"
                     /locus_tag="I9760_001735"
     CDS             complement(1494..2093)
                     /gene="ruvA"
                     /locus_tag="I9760_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAT5043503.1"
                     /translation="MIGWLHGQIIDKHQPGKLVLDVNGVGYDVETSLNTFFQIENGNQ
                     PVGLHIHTIVREDALLLYGFLDKEERSLFRSLIKVNGVGPKLAMTVLSSISPKEFIQC
                     IHQENAALLTKLPGIGKKTAERLVVEMRDSIKQFDGSVSDTFQKQAGSTHSQQEAISA
                     LEALGYKPQEAWKVMNKIDNGNKSCEQLIREALQILSSR"
     gene            complement(2090..2614)
                     /gene="ruvC"
                     /locus_tag="I9760_001736"
     CDS             complement(2090..2614)
                     /gene="ruvC"
                     /locus_tag="I9760_001736"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAT5043504.1"
                     /translation="MTIILGIDPGSRVTGYGLIKESDRKIAYIDSGCIRTSNDTELSH
                     KLLQIYDGICELMDHYSPTEVAIEQIFMHNNPNSALKLGHARGVAMVAAASHRAKIFE
                     YSAREIKQSVVGYGAAEKDQVSHMVVELLQLNRAPQKDAADALAIAICHSHMRNGLSK
                     LIGSRGTKRRRMRL"
     gene            complement(2614..3357)
                     /locus_tag="I9760_001737"
     CDS             complement(2614..3357)
                     /locus_tag="I9760_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAT5043505.1"
                     /translation="MAGHSKWANIKFRKGVQDAKRGKIFTKLIREITVAARMGGGDES
                     SNPRLRDAVKKALNANMKRDTIDNAVKRGVGGVDGEAMIAMRYEGYGPGGVAILVDCL
                     SDNKNRTVSEVRHAFSKHGGNLGTDGSVSYLFTNQGEILMASNQPEDKVMEIAIDAGA
                     SDVAVEDGQIEIITPVEAYHTVLNALQDAGLEVEQSHLTMRAQTLVPINDETAESLIK
                     LIDMLEDLDDVQEVYSNAEFSEKILESMN"
     gene            3503..4753
                     /locus_tag="I9760_001738"
     CDS             3503..4753
                     /locus_tag="I9760_001738"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HAT5043506.1"
                     /translation="MYLQGFGNYHHSEAVKGALPPNQNSPQHCSLGLYAEQLSGTAFT
                     RPRHNNLRSWLYRILPTVTQGTYYPYEFNIMQPFVDELSPNAMRWSPLYNSSQIKCDF
                     VEGLFHIAGSPLVNTYTYYCNHSMSDKYFANNDGELLFVPYAGEIHLHTEFGKLMLSS
                     GSIAVIPRGVKFKVEVISKEAKGYLCENSGNPLTLPQLGPIGANGLANPRHFQYPVAA
                     FENSGSEHTIICKNQKKLWFTVCNHSPLNVVAWHGNYAPYCYDLSLFNTINTVSFDHP
                     DPSIFTVLTSESEIPGVSNLDFVIFPPRWMVAEHTFRPPYFHRNYMNELMGLVYGEYD
                     AKKEGFIPGGISIHNCMTPHGPDYESYEIAASQDLKPNYINSLAFMFETKDYWQVTEQ
                     AYRHPSRQMDYLNCWQGFKIEFSQ"
     gene            complement(4856..5881)
                     /gene="rpoS"
                     /locus_tag="I9760_001739"
     CDS             complement(4856..5881)
                     /gene="rpoS"
                     /locus_tag="I9760_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAT5043507.1"
                     /translation="MQDDEEPIKDKEFKEEEWSEPDDESLLSEEDVEPDIEKMEEELD
                     ELPEFTDDETFPSFQRGKNIAKALDATQLYLGEIGFSPLLTAEEEIHYATLALKGDME
                     ARKKMIESNLRLVVKIARRYLNRGLPLLDLIEEGNLGLMKSVEKFDPKRGFRFSTYAT
                     WWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKP
                     LEDVQKLLGLNDKVASVDTPIGFDETKSLLDTIADENSLNPAELLTNENLRQHIESLL
                     DKLTENQQQVIARRFGLRGFEKATLEDVGKEIDLTRERVRQIQVEALKTLRTLLERVG
                     LTQEDLF"
     gene            complement(5967..6710)
                     /locus_tag="I9760_001740"
     CDS             complement(5967..6710)
                     /locus_tag="I9760_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAT5043508.1"
                     /translation="MMRLFRKQLICIFFILSAAGCGTRSDLAPVTELKWQPYSKYQKF
                     HTVRSGETLYAIAFRYDTDYRRLAINNHLRPPYSLRVGQVLSLKGIVPKSNHFHSAPR
                     ANLHITHKPKQKTIYSPAYNVRSSSGWLWPVTGRVVTTFIPSQGKKGINIACNRGDKV
                     RASASGVVAYAGSGLAGYGNLIIIKHSNEYLTAYGNNARNLVTEGQRVNAGQVIAEAG
                     LIDRSYWGVHFEIRRAGVPVNPLNYLQKG"
     gene            complement(6729..7484)
                     /gene="surE"
                     /locus_tag="I9760_001741"
     CDS             complement(6729..7484)
                     /gene="surE"
                     /locus_tag="I9760_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAT5043509.1"
                     /translation="MKILVSNDDGVLAPGIKILANELSTLGEVKVVAPDRNRSGASNS
                     LTLTQPLRVKQLDNGYYSVDGTPTDCVHLALTGFLEPTDIVVSGINEGANLGDDVLYS
                     GTVAAAMEGRYLGLPAIAISMVGDNIQHYETAAIIAKQLVIKLSANKLPSQTILNVNV
                     PDLPLNQIRGLQVTRLGTRHSAEPIIKEYDPRGRPIYWVGPPGIEADAGAGTDFFAIK
                     TGHVSITPLHLDMTHYKLFDHLSNLLNEICIEN"
     gene            complement(7487..7870)
                     /locus_tag="I9760_001742"
     CDS             complement(7487..7870)
                     /locus_tag="I9760_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043510.1"
                     /translation="MNKYLVIILITLGLVSCQFKKDESYYRSHPSELQNALKLCPGTQ
                     PDGLSCQQLEAIGRRMNGLAYQLQYNPQEFGNKILALQEVIANQQREIQSKKDNAELQ
                     DSLKRNQEDLAYYLAVVKWLESPES"
     gene            7950..9674
                     /locus_tag="I9760_001743"
     CDS             7950..9674
                     /locus_tag="I9760_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAT5043511.1"
                     /translation="MLDFSLNRDPQTGELYIETSLCGKPLLTTPQLNKGTAFTQEERK
                     DFGLLGKLPHRVETLDEQVKRAYLQYSSYTTRLQQHIYLNNLHDKNQIVFYKLLSRHL
                     GEMLPIIYTPIVGAAAKRFSHEYRQPRGLYIAHSDKNQLEEIINNRSNPDIDLVVVTD
                     GEGVLGIGDQGIGGMDIPVAKLMVYSLCGGIDPTRTLPIFLDVGTNNQELLNDPMYLG
                     CRHPRIKSSEYDDFIKTFVNEIHKQFPNAFLHWEDFGRGNARRILDQFQDELCTFNDD
                     IQGTGAVTLAAILAACDVTGLPLHEHRIVVFGAGSAGTGISDQIVDAMVKSGLSLTDA
                     YDRFWLIDKQGLLLATDQELTDAQKPYARNPIDIQSWEINNKQHPSFTDTIRHVKPTI
                     LIGCSAQTGAFSQDIVETMSTSCERPIIFPLSNPDEKCEAQPSDILAWSKGRALIATG
                     TAFAPIEYQNRMVQIAQCNNALVFPGIGLGVLAVSASRLTKDMILAAAQTLSKFAPSK
                     KDSFLPLLPSLDNAQIVAKEIAIAVAQCAIDSGYAQKNQDKELPRLIDELFWEPRYLP
                     FRKKGWSE"
     gene            complement(9722..10093)
                     /locus_tag="I9760_001744"
     CDS             complement(9722..10093)
                     /locus_tag="I9760_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HAT5043512.1"
                     /translation="MKKLFSGLLMGFSALAYANNEVVINVSSKNPSFVVSLAANPTTG
                     YQWSVVEFDKNLLTLSNSIYEKPKTNLIGAGGKMLFTFNLNKGKTYPAQTKMVFKYAR
                     SWEPESGTIKNVTINFANPSD"
     gene            complement(10130..10447)
                     /locus_tag="I9760_001745"
     CDS             complement(10130..10447)
                     /locus_tag="I9760_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HAT5043513.1"
                     /translation="MIIRNYLLAIVFLMIVYPNAYAMRCGDKLVYEGDSKYTVLSKCG
                     EPLDKLVYEENVPLYNAAGYQIGYGTNTVETWIYQKSPVDFQYELIFDYGKLKQINVN
                     RNP"
     gene            complement(10530..12341)
                     /locus_tag="I9760_001746"
     CDS             complement(10530..12341)
                     /locus_tag="I9760_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5043514.1"
                     /translation="MNSDNKKSEELPKTLGRFFWHFIKKQPLAFTVFFVAPIAMVLEN
                     NAIPYSLKMIIDALGMHSREENIFSVVAPALWLGGGAWFVLIVILRLQNWWQGYVIPR
                     FQADVRMSVMEHLSRQSYHYFSNQMAGGLANKVNDLPRALDSIFNIITWYAIAAFASI
                     IVALILMCTINYWFAVILLSWIIFQLYISYRLSKKVDQYAKENAEDKSQLSGKIVDSL
                     SNASVVKLFARSRDELAYISASQNMEVQSNKKLTIYMNIFRLYLDIPVTVMLVAMVYF
                     LLTFWNKKLITTGDLVFIFNVSFSIMTQIWYLCHAIAEFFREIGIARQAIALLSAPIE
                     VQDVPDAKPLNVKEGKIEFDSVTFHYNQGKKVFENKSVIIKPKQRVGLVGFSGSGKTT
                     FIHLILRLFNVESGKILIDGQDISLVTQDSLRSAISLIPQDTTLFHRTLMENIRYGNP
                     DATDDEVVMASKQACCDDFISLLPDGYNTLVGERGIKLSGGQRQRVAIARAILKNTKI
                     LILDEATSQLDSLTEEAIQNSLWQLMESKTTIVIAHRLSTLLHMDRILVFEAGKIIED
                     GSHEELLAKDGLYKSMWDAQVGGFLPESDRDRDNWDE"
     gene            12577..>13947
                     /locus_tag="I9760_001747"
     CDS             12577..>13947
                     /locus_tag="I9760_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAT5043515.1"
                     /translation="MEHETMEFDVIIVGAGPSGLSAAIKLKQLALASQKEITICILEK
                     GAQVGAHILSGAVLEPRSLKELLPDNWQQAPLDTPVNKDLFYFLTQNKSFKLPTPKPM
                     HNEGNFIISLGELCKFLAEQAESLGCEIYPGFAAAEILYNDKNQVIGVATGDVGIDRQ
                     GKKTDNYQPGMHLLAKQTLFAEGCRGQLSQTLMSRYHLRDHSQPQTYGIGIKEIWQVD
                     ASRHEPGKVIHTVGWPLDHATYGGSFLYHLSEQRVAIGFVVGLDYKNPWLNPFGEFQR
                     FKTHPLIKSVLTGGERIGYGARALNEGGWQSLPKLTFPGGALIGDAAGFLNVPKIKGI
                     HTAMQSGMLAAQACFDALSEEKSGQFELSAYPKKINQSWLKQELYAVRNIRPGFKYGL
                     FPGLINAAFETYVTRGYSPWTLKNHSDYKSLIPADKAKKINYPKPDGVLTFDKLSSVF
                     LSNTFHE"
BASE COUNT         4288 a         2925 c         2470 g         4264 t
ORIGIN      
        1 ttttgttgat gaaattaaaa gtatctcata ttccaaatcc agtggttact ccaaacttgt
       61 tacaagacgt atccttgctt caattggaag gagaaattga aaagcaacga atgatagcca
      121 taattgatgg acaggagccc ttagctcgtg cgttaaaata tttatcgatt ggatgcacta
      181 caactgctgc gagaacgaag gagttacaga aaaccatagt tcagcatggt acaaatttgc
      241 aggcctatcg gcatcaagaa aaatgtcgtg aactaggtgg tcggattgtt ctccattgca
      301 gtcaggcaat gcttccaggc atgggtttaa agagtggtat gtgtcacggc ttagctaggc
      361 attgggcaaa cgatgtaata aagaaagaca gatttatggg ctttcgaggg aatcaagagg
      421 tatctattca gccaaccaaa gcaacggata aaatactaca ggcaatacca agttttaatg
      481 attttgtccg tcttaatcct aaaatctatg aaactcaaca gtctcaaaaa gaacatatct
      541 ttcttgagta tgaggataca gtgggatctg aaccagaaaa acagtttgaa gaattcactg
      601 atcgaataat gtctgaatta gacaagtatg gtaaatctgc tacctttgtt agctatgcta
      661 atagcagttc tggacatgtt gttgctatcc acaaacgaac taaaccgaca cctcaaggtt
      721 tcatcatcga ctattttgat gctaatgcag gttggatgca gtttaaagac gataagagtt
      781 ttcaagagtt tttaacctat tacttaaatg atagacatgc aaaagaaaaa cttaagagca
      841 ttgcttttga aaccctgtgt tatcctagct cctacactca tatgttggat aagctctgtg
      901 aaaaagcacc acaacctact cctcagagac aaagagcaca gagcttgtca gcagaaacag
      961 aaagaaacac tcctcaaata aagtcacaaa ttagcgaaaa aactgagaaa actgagaaaa
     1021 ctgagaaaac tgagaaaact gagaaaactg agaatgagca aagcagaaat acaagaggat
     1081 ttcctattta aagaaagctt ctaaggtgat gatagtacca cctttagctt ggagaatctg
     1141 cttcctgttt ctgggtatcg gtcgcagtct ttgcttcttc ttccttcttt tgagcattat
     1201 agttcgcttc ctttacactt tttttatgct ctttcatctg ttgggaagta accttttctg
     1261 tggtatttga agaggctgca tcggctgtaa tagccgttga ttgctctgtg tttctctgaa
     1321 ttgacatacc tggggtcact ttacccttat cagtttggtg tttatgagta ttgacttgag
     1381 aatcagcagc attgacagca aaggttagca tcacacttac cataaaaccg atcattcttt
     1441 tcataaatgc tcctgtttat tatcgagaat tttatcagat cacagttaca atctcatcgc
     1501 gaagaaagaa tttgcaaagc ctcacgaatc aattgctcac aactcttatt gccattatca
     1561 attttattca taactttcca cgcctcttgt ggcttatagc ctaatgcctc caacgcgctg
     1621 attgcctctt gttgactatg agtgctacct gcctgctttt ggaaggtatc ggaaaccgaa
     1681 ccatcaaatt gttttatact gtctctcatt tcaactacta agcgctctgc agttttcttg
     1741 cctatgccag gtaatttggt taataaagca gcattctcct ggtgaataca ctgaataaat
     1801 tccttaggag aaatactgga taaaacagtc atagccagtt tggggcctac cccattcact
     1861 ttaattaatg atctgaataa agaacgctcc tctttatcca ggaagccata caataacaaa
     1921 gcatcttctc gaactatggt gtgaatatgt aatccgacag gttggttacc attttctatc
     1981 tgaaaaaaag tattcaaaga tgtttctaca tcatatccta cgccattgac atcaagaacc
     2041 aattttcctg gctgatgctt atctataatt tgaccatgga gccagcctat catagtctca
     2101 tcctcctgcg tttcgttcct cttgaaccga ttaattttga caaaccattt ctcatatggc
     2161 tatgacatat tgctattgcc aaagcatcgg cagcgtcttt ttgaggagct cgattgagct
     2221 gaagaagctc tacaaccata tgactcactt gatctttttc agcggcccca taaccaacta
     2281 cactttgctt tatttctctt gctgaatact caaagatttt cgcacgatgt gaggctgctg
     2341 ctaccatggc aaccccccgc gcatgtccta atttcaaagc tgaattagga ttgttatgca
     2401 taaatatctg ctcaatagca acctcagttg gggaatagtg atccattagc tcacatattc
     2461 catcatatat ttgcaataat ttatgactta attccgtatc gtttgatgtg cgaatacagc
     2521 cgctatcgat gtacgcaatt ttacggtcag attctttaat caatccatat cctgtaaccc
     2581 gtgagcctgg atctatccct aatataatcg tcattaattc attgattcta atattttttc
     2641 agagaattca gcattgctgt aaacttcttg tacgtcatcc aaatcctcca gcatgtcaat
     2701 cagtttaatt aaactttcag ccgtttcatc attaattgga accaaggttt gtgcacgcat
     2761 agttaaatga gactgctcaa cctccaaacc ggcatcctgt aaagcattta agacggtgtg
     2821 ataagcttct acaggggtaa tgatctctat ctgaccatct tctacagcaa catcagaagc
     2881 gccagcatcg attgcaatct ccatcacttt atcttctggc tgattagaag ccatgagaat
     2941 ttcaccttga ttagtgaaaa gataggatac tgaaccatca gtgcctaaat tacccccatg
     3001 ttttgaaaaa gcatgtcgaa cttccgatac ggttcgattt ttattgtcag acaaacaatc
     3061 taccagtata gcaacaccac ctggcccgta accttcataa cgcatggcta tcatagcctc
     3121 accatcaact ccccctaccc ctcgcttgac agcattatct atggtatcac gcttcatatt
     3181 agcattcaga gcctttttaa cagcatctct caagcgtgga ttacttgact catctcctcc
     3241 ccccatacgg gcagctacag taatttcacg aattaattta gtaaatattt ttcctctttt
     3301 ggcatcctga acacctttac ggaatttgat attagcccat ttactatgac cagccattac
     3361 atcacctcta aatgaatttt tatttggaga ttatacaatg ctctaacacc aaaaagaatg
     3421 actaaagcca tcgatgctct cgacaatttg aacttttttg gactatgcta gcaccatcaa
     3481 aaatcttgaa ggagattgaa ctgtgtattt gcaaggattt ggtaattatc accacagcga
     3541 ggctgttaaa ggagcactac ccccaaatca aaactcaccg caacactgta gcttaggact
     3601 ttacgcagaa caattaagtg gaaccgcatt cactcgtccc cgacataata atcttcgaag
     3661 ttggctatat agaatacttc ctactgttac ccagggcacg tattacccct atgagtttaa
     3721 tattatgcaa ccttttgttg atgagttgtc acccaatgcc atgcgttggt cacctcttta
     3781 taacagctct caaattaaat gtgattttgt tgaaggacta tttcatattg ccggtagccc
     3841 gttagttaat acctatactt attattgcaa ccactccatg agcgataaat atttcgccaa
     3901 taatgatggt gagttattat ttgttcccta tgcaggcgag attcatctgc atactgaatt
     3961 tggcaaatta atgctctctt ccggatcgat cgcggtgata cctcgtggcg ttaaatttaa
     4021 agtggaagta atcagcaagg aggcaaaagg ttatctttgt gaaaatagcg gaaatccctt
     4081 aaccttacct cagttaggcc ccattggagc caatggtttg gcaaacccaa gacattttca
     4141 atatccagta gccgcatttg aaaactctgg cagcgagcat actataatct gtaaaaacca
     4201 gaaaaaatta tggtttactg tatgcaacca ctctccttta aatgtcgtcg cctggcatgg
     4261 caattatgca ccatattgtt atgatctcag tttgttcaat acaattaaca cagtcagttt
     4321 tgatcaccct gatccttcca tattcactgt attaacttca gaaagcgaaa tacccggtgt
     4381 ttctaacttg gactttgtta ttttcccacc tcgctggatg gttgccgaac atacttttag
     4441 accgccttat tttcatagaa actacatgaa tgaactgatg ggacttgtct atggtgaata
     4501 tgacgccaag aaggaaggat tcataccggg tggtatcagc atccataatt gcatgactcc
     4561 acacggacct gattatgaat cttacgaaat tgcagcgtcg caggatctaa aaccaaatta
     4621 catcaactcc ctagccttta tgtttgaaac caaagactac tggcaagtaa ctgagcaagc
     4681 ctatcgacat cccagtaggc aaatggatta tcttaattgt tggcaaggct ttaaaataga
     4741 gtttagtcaa taaattgact gctttttaag atgtaaaatt gacggtaatt aattaatttg
     4801 gtttcagttg ggtcaaattt gacccaactt gtgtgtaatt aattaactgg ttaaatcaaa
     4861 acaaatcctc ttgtgttagt ccaacccgct ctaacaaagt tctaagtgtc ttcaacgctt
     4921 caacttgaat ttggcgaacg cgttctcggg tcaaatcaat ttccttacca acgtcttcca
     4981 gtgtcgcttt ttcaaaacct cttaacccaa aacgtcttgc gataacctgc tgttgattct
     5041 cagttaattt atccagaaga gattctatat gctgccttaa attctcattg gttagcagct
     5101 ctgcaggatt taaactgttt tcatcagcaa ttgtatctaa taaagatttg gtttcatcaa
     5161 atccaattgg agtatcaact gaagcgactt tatcattcaa tcccaataat ttttggacat
     5221 cctccaaagg cttatccacc atttctgcta tttcttcagg tgatggttca tgatccagtt
     5281 tttgcgtcaa ttgccttgcc gctctgagat aaacattaag ttctttcacg acatgaatag
     5341 gcaaacggat agttctcgtt tgattcataa ttgcgcgctc aatagtttgc ctaatccacc
     5401 aggtcgcata agttgaaaaa cggaatcctc gcttggggtc aaatttctca acagacttca
     5461 ttaaacccag gttaccctct tcgattagat caagaagagg caatcctcta ttaagataac
     5521 gtctggctat tttaaccacc aggcgcaaat tcgactcaat cattttcttg cgtgcttcca
     5581 tatcaccttt taaagcaaga gtcgcataat ggatttcttc ttcagccgtt aacaaaggag
     5641 aaaaaccaat ttcacccaag taaagttgag tcgcatctaa agccttggca atatttttcc
     5701 cgcgctggaa tgaaggaaaa gtttcatcat cagtaaactc tggaagttca tcaagctctt
     5761 cttccatttt ttcgatatca ggttcaacat cttcttctga caacagagat tcatcatctg
     5821 gctcagacca ttcctcttct ttaaattcct tgtccttaat tggctcttca tcatcttgca
     5881 ttgtatcacc tatatgttta gctatgcctt gaaatagttt tttacttctt aacaagccta
     5941 ttgttgctat aactatgagt tatatgtcaa cctttttgta gataattcaa tggattaact
     6001 ggcacccctg ctcttctaat ttcaaaatgc acaccccagt atgatctatc tattaaccca
     6061 gcttctgcta ttacctgccc tgcattaacg cgctgtcctt ctgtcaccag gtttctggca
     6121 ttgttgccgt atgcagttaa atattcatta ctgtgtttaa tgataattaa attaccatac
     6181 ccagccaaac cgctgccagc ataagccaca actccactcg ctgaagctct aactttgtct
     6241 cctctgttac atgcaatatt tatgcctttt ttcccctgag aaggaatgaa tgttgtaaca
     6301 acacgaccag taactggcca tagccaacca gaggaagaac gtacattata agctggagaa
     6361 taaatagttt tttgttttgg cttatgggta atatgtaagt tagctctggg agctgaatga
     6421 aagtgatttg attttggcac gatgcctttt aaacttaaaa cttgtcctac tcgcaaagaa
     6481 taaggtggcc ttaggtgatt gttgatcgca agccgcctat aatcagtatc ataacgaaat
     6541 gctatagcat aaagggtctc acctgatcgc accgtatgga atttctgata ttttgaataa
     6601 ggctgccatt ttagctcggt aacaggagcc aaatcacttc ttgtcccaca accagcagca
     6661 cttaagataa aaaaaataca aattaattgt ttgcgaaata ggcgcatcat tatctcattt
     6721 attatttatt aattttctat gcaaatttcg ttaagtaagt tagataaatg atcaaatagc
     6781 ttataatgag tcatgtctaa atgaagcggt gttatcgaaa catgacctgt tttaattgca
     6841 aaaaaatcag taccagcacc agcatctgct tctatacccg gtggaccaac ccaataaatt
     6901 ggccttcctc tgggatcata ttctttaata ataggctcag cgctatggcg agttcccaat
     6961 cgcgtaacct gcaatcctct tatttgattc aaaggtaaat caggaacatt aacatttaat
     7021 atagtttgag aaggtaattt gttagcactt aattttataa ccaattgttt ggcaataatt
     7081 gcagctgttt cataatgttg tatgttgtcc cctaccattg agattgcaat agcaggcaac
     7141 cctaaataac gaccttccat agccgctgcc actgtacctg agtaaagtac atcatcccct
     7201 aaattggcac cctcattaat ccctgaaaca actatgtctg taggctctaa aaatccggtt
     7261 aacgccagat ggacgcaatc agtaggtgtt ccatccacac tataataacc attgtccaat
     7321 tgtttaactc ttaaaggttg tgttaaagtc aaagagttac tggcgccact tctatttcta
     7381 tcaggcgcaa ccactttaac ttctcctaaa gtggacagct cattagctaa aattttgata
     7441 ccgggcgcca aaaccccgtc gtcattgctt actaatattt tcatgtttaa ctctcagggg
     7501 attctaacca ttttacaact gctaaatagt aagcaagatc ttcttgattt cttttcaatg
     7561 aatcttgtaa ttcagcgtta tcctttttac tctgtatttc ccgttgctga ttggcaataa
     7621 cctcctgcaa agccaaaatt ttgttaccaa actcttgtgg attgtattgt agttgataag
     7681 caagaccatt catcctcctg ccaattgcct ccagttgctg gcaactcaat ccatcgggtt
     7741 gcgtgccagg gcacaatttt aaagcatttt gcagttcgga tgggtgggaa cgataatagg
     7801 attcgtcttt tttaaactga catgaaacca atcccaaagt tattaatatt atcactaagt
     7861 acttattcat acccgccctc ttgatgtaaa ctacaaaaag tcgatattat caccagtcag
     7921 caagtaacgc tatcttatga gaataccata tgcttgattt tagtttgaat cgtgatcccc
     7981 aaacgggcga gttatacata gaaacctcat tatgtgggaa gcctttactg actacaccgc
     8041 aattaaacaa aggaacagca tttactcagg aagaaagaaa agatttcggt cttttaggca
     8101 agttaccaca ccgcgtagaa actcttgatg aacaagtaaa acgtgcctat cttcaatatt
     8161 caagttacac gacacgtttg cagcaacata tttatttgaa taatttacat gataaaaacc
     8221 aaatagtatt ttacaaactg ctaagtcgtc atttggggga aatgcttccc atcatctata
     8281 caccaatcgt tggtgctgca gccaagaggt ttagtcatga atacagacag ccacgaggat
     8341 tgtatattgc tcattcggat aaaaaccagc ttgaggaaat aattaataac cgttcgaatc
     8401 cagacataga ccttgttgta gtgaccgatg gtgagggagt cctaggcatt ggcgatcaag
     8461 ggataggagg aatggatata cctgttgcaa aactaatggt ttacagctta tgtggtggga
     8521 ttgatccaac tcgcacatta cccatatttc ttgatgtagg aactaacaac caagaattat
     8581 taaatgaccc tatgtatctg ggttgccgtc accccagaat aaaaagctca gaatacgacg
     8641 attttatcaa aacttttgtc aatgaaattc ataagcaatt tccaaatgct tttttacatt
     8701 gggaagactt tggtcgaggc aatgctcgac gaatcctgga tcaatttcaa gatgaattgt
     8761 gtacttttaa tgatgatatt cagggaactg gtgcagtaac tctggcagca attttggcag
     8821 catgtgatgt aactggacta cccttacatg aacacagaat cgttgtgttt ggagctggct
     8881 cagcaggtac tggtattagc gaccaaattg ttgatgcgat ggtgaaaagc ggattgagcc
     8941 taactgatgc ttatgacaga ttctggttaa tagacaaaca ggggttatta cttgcgactg
     9001 accaagagct tactgatgct caaaaaccct atgcacggaa tcctatagat atacaatcct
     9061 gggaaattaa taataaacaa catccttcgt ttactgacac tatcaggcat gtcaaaccta
     9121 ccattttaat tggctgttca gcgcaaacgg gtgcattctc acaagatatt gtggaaacta
     9181 tgtcgacatc ctgcgagcga ccaatcattt tccctttatc aaatcctgat gaaaaatgtg
     9241 aagcacagcc ctcagatata ttggcatgga gtaaaggacg ggcattaatt gctactggaa
     9301 ctgcttttgc ccctattgaa tatcagaacc gaatggtgca aatagctcaa tgcaacaatg
     9361 ccttggtatt ccctggaatt ggattgggcg ttttggcggt aagcgcctct cgtttaacca
     9421 aagatatgat attggctgcc gcccagactt taagtaaatt tgcgccaagt aaaaaggaca
     9481 gctttttgcc cctgttaccc tcattagaca atgcgcaaat tgtagccaaa gaaatagcta
     9541 ttgcagtagc tcagtgtgca atagactcag gttatgctca aaaaaatcaa gacaaggagc
     9601 tacctcgatt aattgatgaa ctcttttggg agcctagata tctgcctttt agaaaaaaag
     9661 gatggagtga atagaataaa gctctatgac tatattattg aaaaataaat tttcttaatt
     9721 cttagtctga ggggttggcg aaattaatcg ttacattttt tatagtcccg ctctctggct
     9781 cccaggaacg agcgtattta aagaccattt tggtttgagc gggataggtt ttacctttat
     9841 tcaagttaaa agtaaatagc attttaccac ccgcacctat caaattagtc ttgggctttt
     9901 cataaatgct attacttaag gtaagcaagt ttttgtcaaa ttctactaca gaccattgat
     9961 aacctgtagt tggattagct gcaagactga caacgaaact gggatttttt gagctgacat
    10021 tgatgacaac ctcgttattt gcataggcca gagcagagaa gcccataagt aatccgctga
    10081 ataatttttt catatttttt tcctcacaaa gcttcttaca cgctcaccat tatgggttac
    10141 gatttacgtt aatctgtttt aatttcccat aatcaaaaat taattcgtat tgaaaatcaa
    10201 caggagattt ttgataaatc caggtttcga ctgtattagt accataacct atctgataac
    10261 cagccgcgtt gtataatggc acattctcct cataaaccaa tttatcaagt ggctcaccac
    10321 atttagacaa aacagtgtat ttactatccc cttcatagac caatttatct ccacacctca
    10381 ttgcatacgc attaggatat acaatcatta aaaagacaat agccaacaaa taattccgga
    10441 tgatcattcc ctaccccttg agaattcaat gctccttgca acaaaatttt caaaaagtct
    10501 atttttatag catagcactt ggtatctgat tattcatccc aattgtctct gtctcgatcg
    10561 gattcaggca agaatccacc tacttgagca tcccacatgg atttatataa accatccttc
    10621 gctaataact cttcatggct tccatcttca attatttttc ctgcttcaaa aactaaaatt
    10681 ctatccatat gcaatagtgt tgataatcgg tgggcaataa caattgtagt ctttgattcc
    10741 atgagttgcc acaatgaatt ttgtatggct tcttcggtta gggaatccag ctgtgaagta
    10801 gcctcatcta atatcaaaat cttcgtattt tttaaaatgg cacgagcaat tgcaacgcgt
    10861 tgtctttgtc ctcctgataa tttaattcct ctttcaccca ccaaagtatt ataaccatcc
    10921 ggtaaaagtg aaataaaatc atcacagcat gcctgttttg atgccatcac aacctcatca
    10981 tcagtcgcat caggattacc ataacgaatg ttttccatca aagtcctatg aaacaaagtg
    11041 gtatcttgag ggataaggct gatagctgag cgcaaggaat cttgagttac cagactgata
    11101 tcctgaccat caatcaaaat ttttccagat tccacattga ataaacgtaa aattaaatgg
    11161 ataaacgtcg ttttaccact tcccgaaaaa cctaccaagc caacacgctg tttcggctta
    11221 atgattactg atttattttc aaatactttc ttaccctggt tataatgaaa tgtaacgcta
    11281 tcaaactcta ttttgccttc tttaacattc aatggcttgg catcaggaac atcttgaacc
    11341 tctatgggtg cactaagcaa agcaatagct tgtcttgcta tgcctatctc tcgaaagaat
    11401 tcagcaatag catggcacaa ataccaaatc tgagtcatga tagaaaacga tacattgaat
    11461 ataaagacca agtcgcctgt tgtaatgagt tttttattcc aaaaagttaa caggaaatac
    11521 accatagcca ctagcatgac tgtgactgga atatccagat acaaacgaaa tatgttcata
    11581 taaatcgtca gttttttatt actttgtacc tccatattct gacttgcgga aatataagcc
    11641 agctcatctc gagatcgagc gaaaagcttc actacactgg cattactcaa actatcaact
    11701 attttaccgc ttaactgact tttatcttct gcgttttctt ttgcatattg atcaactttt
    11761 ttagataatc tataactgat gtaaagctga aaaataatcc agcttaacaa aataaccgca
    11821 aaccagtagt taatagtgca catcaaaatt aatgccacaa tgatagaagc gaatgcggcg
    11881 attgcatacc aggttataat attgaatatg gaatccagag cacggggtaa gtcattgact
    11941 ttgttagcta aaccacccgc catttgattg gaaaaataat gataagactg gcgactcaaa
    12001 tgctccataa cagacattct gacatcagcc tgaaatcttg gtatcacata accttgccac
    12061 caattttgca gacgtaaaat cacgatcaaa acgaaccagg caccgccacc taaccataaa
    12121 gccggagcaa ctactgaaaa aatattttct tccctggagt gcatcccaag agcatcaatg
    12181 atcattttca aagaataggg aatagcatta ttttcaagca ccattgcaat cggtgctaca
    12241 aaaaataccg taaaagccaa aggctgtttt tttataaaat gccagaaaaa ccgacccagt
    12301 gtttttggca actcttcaga ttttttatta tcgctattca taaaccatac cttcaatgaa
    12361 ttttgacaag gttcgattat tctaacctaa tatttgctaa ttggagaatt tatgtttaaa
    12421 aaacaattat atgccctatc aattcagcta ttctctcaga tttgcattgc catgaaataa
    12481 aattagccct tcaaaaattg aaaatataaa atttaatctt gatcttaaac cttataaagg
    12541 cgatcatttt ttcatttgta agttagagga gtacgtgtgg aacacgaaac gatggaattt
    12601 gatgttatca tagtcggtgc agggccttct ggattatccg ctgcaatcaa attaaagcaa
    12661 ctggctttag cttcacaaaa agaaattact atttgtatcc tggaaaaagg agctcaggtt
    12721 ggcgctcata ttctttctgg cgcagtttta gagcctagaa gcttaaaaga attactacca
    12781 gataattggc aacaggcccc actggatact cctgttaaca aagatctgtt ttattttttg
    12841 actcaaaata aatcatttaa gctccctact ccaaaaccta tgcataatga aggaaatttt
    12901 attatcagtt taggggaact atgtaagttt cttgcagaac aagcagagtc tctaggctgt
    12961 gaaatatacc ctggatttgc tgcagcagaa attctctaca atgataaaaa tcaagtcata
    13021 ggtgttgcta ctggggatgt aggaattgat agacaaggga aaaaaactga taattatcaa
    13081 cctggaatgc atttattagc caaacaaact ttatttgctg agggatgtcg cgggcaatta
    13141 agtcaaacat taatgagtcg ctatcattta agagatcatt cgcagccaca aacttacggc
    13201 atagggatta aagaaatctg gcaagttgat gcatcgcgcc atgaacctgg aaaagttatc
    13261 catacggttg gttggcctct tgatcatgcg acttatggag gctccttttt gtatcatctt
    13321 tcagagcaga gagttgcaat aggttttgtt gttgggcttg actacaagaa cccatggctc
    13381 aatccctttg gcgaatttca gagatttaaa acgcatcccc ttattaaatc tgttctaaca
    13441 ggaggtgagc gaattggtta tggagccaga gcacttaatg aaggaggatg gcaatctctc
    13501 cctaaactta cctttcctgg cggcgccttg attggagatg ctgccggttt cctaaatgtg
    13561 cctaaaatca aaggaattca caccgcaatg caatctggaa tgcttgctgc acaagcttgc
    13621 tttgatgcgc taagtgaaga aaagagcggt caatttgaac taagtgcata ccctaagaaa
    13681 atcaatcaat cctggctaaa gcaagagctc tatgcagtgc gaaatattcg accaggattc
    13741 aaatacggtt tatttccagg tctaattaat gctgccttcg agacttatgt gactcgagga
    13801 tattctccat ggaccttaaa aaatcattct gactataagt cgttaattcc agcagacaaa
    13861 gctaaaaaaa ttaactaccc aaaaccagat ggcgtactca cgtttgataa actctcatct
    13921 gtatttttat caaatacatt tcatgag
//
LOCUS       DACTJE010000077        13888 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.077, whole genome shotgun sequence.
ACCESSION   DACTJE010000077 DACTJE010000000
VERSION     DACTJE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13888
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.077"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..226)
                     /locus_tag="I9760_001748"
     CDS             complement(<1..226)
                     /locus_tag="I9760_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043516.1"
                     /translation="MSRAKFFKSNRTHVIELYCYSNEYAQQVNHEITSGADSGPLLTK
                     IYGQDVRFIYAPDSEKFNLVLNEARKRNYNQ"
     gene            666..920
                     /locus_tag="I9760_001749"
     CDS             666..920
                     /locus_tag="I9760_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043517.1"
                     /translation="MNSYAAKVTPVEEHIAQTTSPQFMSIFLLVQILSILIGVACLFV
                     AFYILRPRKKDQMLIQDQFLLYLILGLFFIFSPFLLEYLR"
     gene            complement(978..1592)
                     /gene="lem15"
                     /locus_tag="I9760_001750"
     CDS             complement(978..1592)
                     /gene="lem15"
                     /locus_tag="I9760_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem15"
                     /protein_id="HAT5043518.1"
                     /translation="MIYKITLTDISLAQAFISIPQDVIALDLSNNLLGSKSCVDLLVN
                     IADCIPAGVTSLDLSSNGLRLTQVSLVTPKHVTSLNLCFNPLHEISIDGLEWLKDSLA
                     HIQIIYLSYDMVNNMSREQRQAFRAVIPNIQTIILVDKNGKEIGPSHTAKIANLISEL
                     GGGNPEVPSLLNQCTFFVKTQGINIDKAYIPEELKKRIHDCNPL"
     gene            complement(1742..3043)
                     /locus_tag="I9760_001751"
     CDS             complement(1742..3043)
                     /locus_tag="I9760_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HAT5043519.1"
                     /translation="MKMIGDKSVLDVYLGESQIANITLIDDQLYWSYHESWQQTGYAI
                     SPHLPMHEDIPPLNVQRFLRNLLPEGNPLEVLVNVFHLSKYNTFGLIQALGLDTPGSL
                     VILPSNQAIPKHANFRLVTHEELEDRLNHCDSYNLIVWDGKPRLSVAGVQDKINVVLN
                     KEGQLGFGEGSLCSTHILKFETQKLSYLVLNEFLSMWLAKCCELNVANVQMKRFGQNP
                     ALLVERFDRKLITTNIVKRRHLIDGCQAMNLPPEFKYEQNFGNNRDVAHIRDGVSLPK
                     LFDFANQCINPAKTKQQILDWVLFNVLIFNCDAHGKNISFFVGVNGISLAPFYDLVNI
                     KMYPEFEHALAMALGDEFDGDNINAYQLADFADTCRLPRSLVAKHLKYLIGKLTTALQ
                     EETQFNLVGNEEDYFKKYQEIVMKRCEHLLKQNDQITSIKL"
     gene            complement(3024..3305)
                     /locus_tag="I9760_001752"
     CDS             complement(3024..3305)
                     /locus_tag="I9760_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT5043520.1"
                     /translation="MAKQIKKLKTPDLQQPLTAELLGEVIKARRTQSNLRLEDAAALC
                     GVAKETFMKIEHGQSNCQLASVLQICSGLGINLYIKPWMDNGEDENDWR"
     gene            complement(3546..5438)
                     /locus_tag="I9760_001753"
     CDS             complement(3546..5438)
                     /locus_tag="I9760_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HAT5043521.1"
                     /translation="MPDDISRKQFSIGDLYCTNELEVSGLIYEIFHQKSYLSDFLTLS
                     PGAVVFDVGANIGIFSLFVLKQCHYDTEIYSFEPVPATFKCLKKNLARFKHNVHVYNA
                     GIGNVPKDCSIDFTLFGESSVTATYKPTDKIISNYQPLLNYETLLKLSSFQNKPLYYQ
                     LKYLPFLRNYLIKKNYKHQTLETKVRCHLTALGRFIENNQITRIDLLKIDVEGAELDV
                     INSIKPEQFSLIKQLSIEVHDIDNRVEKLVSYLQKQGYITYVDRNPIFAELGFNHHMI
                     YAKLPEPAIITLSEEANNSENYIEARQYFYGLLAGGVRIKLLESMFDLDLFRLFTGKP
                     YLLENDIIKRLELKPVRAKKWLHLLCCEDFLKKIMVGSQVGYQLAKSQLMLGEGYWGF
                     KQYYDFYWQRMANEKLSNILRFTDPKFNVSWPPKTAEEANFLETWMTKTATPLIQTLL
                     ACLDLNQYRSILDVGGGDGTIACALAQAYPHLKITVYNLPESAKIAQKNIDAMGLQKR
                     ISVFSGDFINDEQFPAGFDLILFVRVLWDWDNSRKRKLLNMAYHALKRKGHVAICEGF
                     KELCYDLCLTWEYSYIFADGFDAEVFKTSDEYKIMLQQIGFTPIPIKSISPSEPVPGT
                     VVLAEK"
     gene            complement(5453..6496)
                     /locus_tag="I9760_001754"
     CDS             complement(5453..6496)
                     /locus_tag="I9760_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HAT5043522.1"
                     /translation="MAYHQLSYTFLNHQKLPLIIQPQAKAEGNIDVLKQFLKEENTLF
                     KEQLLNYGAIILRGFHVNTAEQFLEVIRSSDLGPNYSYDFCPIPRTQIRENIYTSINI
                     HPSFNLALHNEKSYDQDFPSHIFFNCIHAPQTGGDTALADGNKIWFSLPKLLQKKLQS
                     KGILYRRHYYGSGIQYKIIRSIGKNSGCMTWMERFQTDDQNKVEVMLQQMGVQFRWIQ
                     GNGLIIEQLLPACRNHPISGKLVWFNQSNHANHYYNGTSDYIKSKINNTFSRFILLHK
                     YFHPYIAFYGDGEPLSKQEADCINSAIQKNTVSTAWQPGDVMIVDNFSCLHGKTPHTG
                     NRLILVGLTKYFD"
     gene            7142..8623
                     /locus_tag="I9760_001755"
     CDS             7142..8623
                     /locus_tag="I9760_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HAT5043523.1"
                     /translation="MSFFDLLIKKANHLVSQIYKKNYNSITPDQLIWQHIQDCEKQFQ
                     KNQEAIYYSKDILQDLINKRLQTILLHAKTKSPWYKKTLAKINIENFTRERLEELPTI
                     NKTILMENWDAIVTDPKLSLNLVEKHLSKKNDSIDTLYLFSRYQVVSTGGSSGKRGVF
                     IYDWDEWITFHTAFIRYPLYNYERTQIVTTKLNTNPRIVSLFVTNTAIAAYSLAKTFG
                     RGNQNMYFLPMAVTPLNIVITRLNQIQPDILSAGPSYIHKVCQLVQKGQIKIEPKILF
                     VGGEPLFEQTLALIKETWPKVDIFNVFGCTEGMVGLNCRANVDEMHLNDDQCIIEPLD
                     EQNRPVDKGIMVSKMYITNLYNYTLPLIRYENSDEILFLNKTCDCGIHHQLIQTPKGR
                     PGCDFNYPGNIFVHHSLFLGALLPEKNIQEYQVEQTIDGANIRVVTTGFINKSQLQKN
                     IRLRLSKVGLMDAQVNITEVPEIKYLYSGKLNRFIPLNSTDKS"
     gene            complement(8935..10251)
                     /locus_tag="I9760_001756"
     CDS             complement(8935..10251)
                     /locus_tag="I9760_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HAT5043524.1"
                     /translation="MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSE
                     EQLHRSGISEQLIANPNYKPYRGILDDLESFDTAPFENTTKNFELLGVQGKVMSHLTH
                     RALSTMRSQEHHSNNYIKNTAVYIGANNQPAAHFLGAAYYQSIDTQKAIERYYSKVIA
                     PYISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYL
                     YEENGLFSSDGHCHSFDASACGTLYSNGAGLVVLKRLEDAIADHDLIHAVIIGSAMNN
                     DGNSSKEGFMAPGVHGQYTCLQSAWRNAEVEPDDLDYIAAHGSSTKLGDATEIYALKK
                     AFKDVKKTYRCPISSVISNLGHTGIVSGMASIIKTVLMLKNKAIVPSINFETPNPDFM
                     LEDSPIYIATEFQDFPTVKKHYLAGVSSYGAGGSNTHLVLRSYDEN"
     gene            complement(10422..11942)
                     /locus_tag="I9760_001757"
     CDS             complement(10422..11942)
                     /locus_tag="I9760_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HAT5043525.1"
                     /translation="MRSPDQDTTICSLFEEKVKQYPENTAVYLEDRQISYDAFNKQVN
                     QYARYLQRKGLPLNGIVGIQLTRSFEMLVAIFAVLKAGGAYLPIDPMAPVIRNQTILE
                     DSQIKFLIVSDNSLLTQKAGLQVLSIHANVSDEDCHNLNLPREKQDLAYVMYTSGTTG
                     KPKGVMISHQALVNRILWMQNTFPLQTHDVVFHKTHICFDISVWETCWWSIAGAGVVL
                     LPPGKEHDIKLFIQMIEQYQINVIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGE
                     ALDAKSVNLFNKLLQDQKTILVNLYGPTEATIDVSCFICEKHKDYRTVPIGKPIQNTQ
                     FFVLDEQLNSTGFEPGELYISGVGLAQGYLNNPALTRTSFIDNPYLPGEKMYRTGDIV
                     RWNENHELLFLGRRDDQVKLHGIRIELGEIQHHLLDHPNIQDAVVVCEKVEGLDYRMV
                     VFLIANNQTKIGSAELKSFLKTRLPDYMVPEHYLWLSAFPIKENGKIDKEKLMTFI"
     gene            complement(11959..13086)
                     /locus_tag="I9760_001758"
     CDS             complement(11959..13086)
                     /locus_tag="I9760_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT5043526.1"
                     /translation="MPNYQPRQVKKQQFFNQFIENCLHPHAKKFDKEQNIPRDFFYTL
                     AQNGFLGACIPKQFGGQQWDELTIGIMYESFAKELCSLANILTVLGMVSKSLLQFGSK
                     AQKQIWLPRIASGETLAALALTEPNIGSDLEHVETQLLDAGDEFILNGSKKYITLGQI
                     ADLFLVLANCQGQPTAVLVEKGTPGLIISPMSNFLGLRSNMLAEIFFDNCRIPKTNLL
                     GSIGVGFTYVIQTALDEGRYTTAYGCVGLGQACLDAALGYTQERKQFNRRLDEHQLIQ
                     KMITEMVVQVKAARELCFYAGELRQKKNPAYIAETLIAKYAASKMVVFVSNHALQLFG
                     AAGFDETYPVERYYRDAKVMEIIEGTSQMHEILISKLCINK"
     gene            complement(13117..>13888)
                     /locus_tag="I9760_001759"
     CDS             complement(13117..>13888)
                     /locus_tag="I9760_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HAT5043527.1"
                     /translation="VTLIDNIQSQLDKAKTTIAKNLHYLALTQNLALTHSIETILASI
                     AFTTQLDELKESEYIIENITENWELKKALYQVLNEQGNPTCILGVNTSSIPITKIASL
                     VEHPQRVIGVHFMNPATIMPMVEVIKGYHTDESTIKKTRTLLEQVHKKMIVVKDSVGF
                     VSNRAMMIFINEAIFMVQENIASIEDIDVLFKQCFGHKMGPLHTADLIGLDTVLYSLE
                     TIYSELNDPKYRPCWLLKNMVDAGLLGQKTKKGFYQYE"
BASE COUNT         4388 a         2692 c         2328 g         4480 t
ORIGIN      
        1 gttggttata atttcttttc ctggcctcgt ttaaaaccag attaaatttt tccgaatcag
       61 gagcatagat aaatctaaca tcttggccat atatttttgt taaaagtggg ccagaatctg
      121 cacctgaagt tatctcatga ttaacttgtt gcgcatattc attactataa caataaagct
      181 caattacatg cgttcgattt gatttaaaaa atttagccct ggacataata tcttttaata
      241 attgcttaaa aatatgtcat tttagcattt aagaattatg attttattaa cctatctatc
      301 aaaaaaatga atttacctcg gtgtagaaca aaatttcata tgccaactgt tgtttttttt
      361 tcagtaggca aattttccga taatttttct aaactcagcg acttttattg caaagttgct
      421 gagtttatct gaataaatca aggtagtatt tgaagaaact gcctcatgga tctttgtggt
      481 ttttatagtt atccacttaa tttggcgttt atttgcctac cccttcccta tgttatgagt
      541 cctgacaaag caatgatcat aaaagcgagc atttttctaa tcgtagagaa agattgttct
      601 catgcacttg aaatcttggt taaatggagc gaggattata acaatctctt tacttaagct
      661 aaaaaatgaa ttcatatgct gccaaagtca ccccggtaga agagcacatt gcccagacca
      721 cttctccaca atttatgtct atatttttgt tagtacaaat tttaagcata ttgattggtg
      781 tggcctgtct ttttgttgca ttttatatac ttcgtccaag aaaaaaagac caaatgctca
      841 tccaggatca gtttttactt tatttaatcc tgggactttt tttcattttt tcccctttcc
      901 tgctcgaata tcttagataa ttagcaaaag ttgtcgggcc aaggcccgac ctacacttgc
      961 tactacgcaa caataaatca gagaggattg caatcatgaa tacgtttttt taattcttcg
     1021 ggaatatagg ccttatctat atttattcct tgagttttaa caaaaaaggt acattggttt
     1081 aacaaagagg gaacctctgg gtttccacct ccaagctcac taatgagatt agcgattttt
     1141 gctgtatgcg aaggaccaat ttctttgcca tttttatcga ccaaaataat tgtttgtatg
     1201 ttgggaatta ccgctcgaaa tgcctgccgt tgctctcttg acatgttatt gaccatatca
     1261 tagcttaaat aaataatttg aatatgtgct aacgaatcct taagccactc taaaccatca
     1321 atagatattt catgtaaggg attaaaacac aaattaagtg aagtcacgtg tttgggtgta
     1381 actaaagaga cttgcgttaa tctgagccca ttgctactca aatcaagcga agtcacaccc
     1441 gcaggtatac agtccgctat atttactaat aaatctacgc aactttttga gcccagtaga
     1501 ttattactca aatcaagggc gattacatct tgagggatag atataaaagc ttgtgccaag
     1561 ctaatgtcag ttaatgttat cttataaatc ataattaaaa cctcattata aatgaaaaaa
     1621 tattattctt attggtcata atatgatata tttgattata attaaaatag attgtttgta
     1681 tattaaatat acaaatctct tatgtgctcc gtattaaagt aagaaataat gaggaaaggc
     1741 gctagagctt tattgaagta atttggtcgt tctgctttaa caagtgttca catctcttca
     1801 tgacaatttc ctgatatttc ttaaaatagt cttcttcgtt accaactaaa ttaaattggg
     1861 tttcttcttg caaagcagtt gttaatttgc caatcaaata ttttaaatgt ttagctacta
     1921 acgaccttgg cagcctacat gtatctgcaa aatcagccaa ctgataagca ttaatattat
     1981 caccatcaaa ttcatcccct aatgccatcg ccagagcatg ctcaaattca ggatacattt
     2041 ttatattaac caaatcataa aatggcgcca gtgatatacc gttaactcca acaaaaaaac
     2101 tgatattttt accgtgagcg tcacaattaa atatcaaaac attaaataaa acccagtcga
     2161 gtatttgttg cttggttttg gcaggattga tacattgatt ggcaaaatca aatagcttgg
     2221 gcaaactgac tccatctcta atatgagcca catccctgtt atttccaaaa ttttgctcat
     2281 atttaaattc agggggtaaa ttcattgcct gacaaccatc tattaagtgc cgtcttttta
     2341 ctatattagt ggtaatcaat ttcctatcaa aacgctctac taataaagca gggttctgac
     2401 caaaacgctt catctgaaca ttggctacat tcaattcaca gcatttagcg agccacatac
     2461 tgaggaattc atttaaaacc agataagata gtttttgtgt ttcaaattta agaatatggg
     2521 tagaacacaa gctaccttct ccaaaaccaa gttgtccttc tttatttaac accacattaa
     2581 ttttatcttg tactccggca actgaaagac gtggttttcc gtcccaaacg attaaattgt
     2641 aagaatcaca atgatttaaa cgatcttcta attcttcatg agttactagt cgaaaattag
     2701 catgtttagg aattgcttga tttgatggca atataactaa tgacccaggt gtatccaacc
     2761 ctaaagcttg aataagtcca aatgtattgt atttacttaa atgaaaaaca ttaactagta
     2821 cttctaaagg atttccttcg ggtaataaat ttcttagaaa tcgttgcaca tttaatggtg
     2881 gaatatcttc atgcatagga aggtgcgggg aaatagcata ccctgtctgt tgccaacttt
     2941 catgataact ccaatacagt tgatcatcga taagtgtgat gttggctatt tgactttcac
     3001 cgagataaac atccaagact gacttatctc caatcatttt catcctcacc attatccatc
     3061 caaggtttta tgtaaagatt tattcctaaa ccagaacaaa tttgtaatac acttgccaat
     3121 tgacagttcg attgcccatg ctcgattttc ataaaggttt ctttggcaac accacataaa
     3181 gcagccgcat cttctaaccg caaattgctt tgagttcgac gtgctttaat gacttcccct
     3241 aacaattccg cagttaatgg ttgttgaagg tcaggcgtct ttaatttttt tatttgctta
     3301 gccatataat cctactaaag tagacttaat ctaagatttc gatttaattt gttaattaat
     3361 cctattatag taggattaat ttcaaatata caatagtaaa tcattaaaat cctattaaag
     3421 taggatttta atataataga ctaacacaac actttaaccg cgttttataa ttttcaaaat
     3481 tacgaaaacc ttgagctctt agttgtgtta catgggtcta tgacaatcgg tatggctgat
     3541 gaaaactatt tttctgccaa tacaaccgtc ccaggcactg gctcggaagg agaaatggat
     3601 ttgattggaa taggagtaaa acctatttgc tgcagcatga ttttgtactc atctgacgtt
     3661 ttaaaaactt ccgcatcaaa tccatctgca aaaatatagc tgtattccca tgtcagacaa
     3721 agatcatagc aaagttcttt aaagccttca cagatagcaa catgaccttt cctttttaaa
     3781 gcatggtaag ccatatttaa taatttcctt tttctggaat tatcccaatc ccacaaaaca
     3841 cgcacgaata aaatcaaatc aaatcctgca ggaaattgtt catcgttaat aaaatcaccc
     3901 gaaaatacac taattcgctt ctgtaatccc attgcatcta tatttttttg agctattttt
     3961 gctgattcag gtaaattgta aacggtaatt tttaaatggg ggtaggcctg agccagtgca
     4021 caggcaatag tgccatcccc gcccccaaca tccaatatgg atcgatattg atttaaatca
     4081 agacaagcca ataaagtttg tataagtggt gttgctgttt ttgtcatcca tgtttcaaga
     4141 aaattagctt cctcagcagt tttcggtggc caggaaacat tgaactttgg atcagtaaaa
     4201 cgcaatatat tacttaattt ttcgttagcc atcctctgcc aataaaaatc ataatattgt
     4261 ttaaatcccc aatacccctc ccctaacatc aattgacttt ttgccaattg atacccgact
     4321 tgcgaaccga ccatgatttt ttttaaaaaa tcttcacaac aaagcaaatg tagccatttc
     4381 tttgctcgaa caggtttcag ttcaagtctc ttgataatgt cattttccaa taaataaggt
     4441 ttgcccgtga acaaacgaaa caaatcaaga tcaaacatag attccagtag tttaattctc
     4501 accccaccag caaggagtcc ataaaaatac tggcgtgctt caatataatt ctcagaatta
     4561 tttgcttctt cacttagtgt gataattgcc ggttcaggca atttggcata gatcatatga
     4621 tgattaaaac ccagctctgc aaaaattggg tttctatcaa cataagtaat ataaccttgc
     4681 ttttgtagat aggagacgag cttttcaacc cgattatcaa tatcatgaac ttcaatactt
     4741 agttgtttaa taagcgaaaa ctgctctggc tttatgctgt ttattacatc aagctcggca
     4801 ccttccacat caatttttag taaatcgata cgagtgatct gattattttc aataaaacgc
     4861 cctaatgcag tcaggtggca tctcactttg gtttctaaag tctgatgttt atagtttttt
     4921 ttaatgagat aattacgcaa aaaaggcaaa tattttaatt gataataaag tggcttattt
     4981 tggaaggaag agagctttaa caatgtttca taatttaaca atggttgata attggagatg
     5041 attttatcag ttggtttgta agtggcggta acggagcttt ccccaaagag ggtaaaatcg
     5101 atactacaat cttttggaac atttcctatc ccagcattgt aaacatgcac attatgctta
     5161 aaccgcgcca aattcttttt taaacactta aaagtagcag ggacaggctc aaaactataa
     5221 atttcagtat cataatgaca ttgctttaaa acaaataagg aaaaaatacc aatattcgcc
     5281 ccaacatcaa aaacaaccgc ccctggactt aaagtaagaa aatcagacaa ataactcttc
     5341 tgatggaaaa tctcataaat taaaccactg acttccaatt cattagtaca atacaaatct
     5401 ccaatactaa attgttttcg tgaaatatca tcaggcattg atatgggtcc ctttaatcaa
     5461 aatattttgt caatccaacc aggattaatc gatttcctgt atggggtgtt tttccatgta
     5521 agcaggaaaa attatcaact atcataacat cccctggttg ccaggctgtg gataccgtat
     5581 ttttttgtat tgcagagtta atgcaatctg cttcttgctt cgataaaggt tcaccgtcgc
     5641 cataaaaagc aatataagga tgaaaatatt tatgtaacaa gataaaacga gaaaaagtgt
     5701 tattaatctt gcttttgatg taatcactgg ttccattata ataatgatta gcatggttac
     5761 tttgattaaa ccagactagt tttccgctga tcggatgatt ccggcaggca ggaagcaatt
     5821 gttctatgat tagcccattg ccttgaatcc atcgaaattg cactcccatt tgctgaagca
     5881 tcacttccac tttattttgg tcatccgttt ggaatcgctc catccaggtc atgcacccag
     5941 agtttttgcc tattgatcga attattttgt actgtattcc agaaccataa taatgccttc
     6001 gatataatat tcctttagat tgcaattttt tttgtaataa ctttggtaaa gaaaaccata
     6061 tcttgtttcc atcagccaat gcagtatctc cgccggtttg cggggcatga atgcaattga
     6121 aaaaaatgtg actgggaaaa tcctggtcat atgatttttc attatgtaaa gcaagattaa
     6181 aactcggatg gatatttatc gaagtataaa tattttctct gatttgtgtt cgcggaatcg
     6241 ggcaaaaatc ataagaataa tttggcccca aatcgcttga ccggatcact tctaaaaatt
     6301 gttcagcggt attaacatga aacccacgta atataatggc gccataattg agaagctgct
     6361 ccttaaacaa ggtattttct tctttaagga attgttttaa aacatcaata ttaccttccg
     6421 ctttagcttg aggctgaata attaaaggta atttttggtg gttcaaaaaa gtatagctga
     6481 gttgatgata tgccataaaa ttcctcactg acaatccgga ggtttactat cagactttca
     6541 gagtgtaggt ccttcctgat tctataagtg gctccctctt tttcacctat attacaaaaa
     6601 ttaccgcatc atctttgacg aatacctata ttaaatagta tagacactga ccttgctatg
     6661 caatgaaaat ccaccagatt ttgccaaaga agctcctgct gaatcactca agcaggactt
     6721 acgaaaaacc ccaaggtcaa ggcaaaaaat gtttttaatt aaactaaatg accgaattaa
     6781 aaacattttt taacatagag attggggttt ttcgtaagtc ctgtcaaatt atctgtggat
     6841 agcatcgact ttaacatcat caccttctaa cttcgatgag aactctttgc aattgcataa
     6901 atcattaaac tgcactgaaa attactgata acggcactac cttatataat agaatatcct
     6961 gcttggtttt aagctataat cttcttaggt attttaatct tatggtgact gccaatcaaa
     7021 agttaatcca ggccacctat tgattaaaat cccgtttagg aatatcacca ctaattatgg
     7081 gttctctcaa agaccatgcc ttatagtgta aggatagcgc cctgggaaaa aggggagtaa
     7141 catgtctttt tttgacctct taataaaaaa agctaatcac ttggttagcc aaatctataa
     7201 gaagaattat aactctataa ctccagatca actgatatgg cagcatattc aagactgcga
     7261 gaaacagttt caaaaaaatc aggaagctat ctattattct aaagacatac tgcaagacct
     7321 aatcaataaa agactgcaaa caatattact tcacgcgaaa acaaaatctc cctggtataa
     7381 aaaaacactg gcaaaaatca acatagaaaa ctttaccaga gagcgattag aggaactccc
     7441 tactataaat aaaaccattt taatggaaaa ttgggatgca attgtcactg atcccaaact
     7501 ttcattaaat ctagttgaaa agcatctgag taaaaaaaat gacagcattg ataccctata
     7561 tttattctcc cgctatcagg tcgtgtcaac tggaggaagc agtgggaaac gcggcgtatt
     7621 tatttatgat tgggatgaat ggattacatt tcacaccgca tttatccgct atccacttta
     7681 caattatgaa cgcacgcaaa ttgtaactac caagctaaac acaaacccaa gaattgtcag
     7741 cctgtttgta acaaatacag caattgcagc ctattcttta gcaaaaacct ttgggcgagg
     7801 taatcaaaat atgtattttc tgcctatggc tgtaacccca ttaaacatag ttatcactcg
     7861 tctcaatcaa atccaacccg atatcttaag tgctggccct tcatatattc acaaagtatg
     7921 tcaattggtt caaaaaggac aaataaaaat tgaacccaaa attctttttg ttggaggaga
     7981 accacttttt gagcaaacct tggctttaat caaagaaacc tggcctaaag tggacatttt
     8041 taatgtattt ggttgtactg aagggatggt agggctaaat tgccgggcga atgtggatga
     8101 gatgcatttg aatgatgatc aatgcattat tgagcctctg gatgagcaaa atcggcccgt
     8161 tgacaaagga attatggtct ccaaaatgta tataactaat ttatataatt acactttacc
     8221 attaatccgc tatgaaaatt cagatgaaat tttattttta aataaaacct gtgattgtgg
     8281 catccaccat cagcttatac aaacccctaa aggtcggcct ggatgtgatt ttaattatcc
     8341 tggtaatatt tttgtacatc attcgctttt cttgggagca ctcttgccag aaaaaaatat
     8401 tcaggaatat caggtcgaac aaacaattga tggggctaat atcagggttg taaccaccgg
     8461 ttttataaac aagagccaat tgcaaaagaa tatacgctta cgattaagca aggtgggttt
     8521 gatggatgcc caagtcaata tcacagaggt acctgaaatt aaatatttgt attcgggtaa
     8581 actgaatcgt tttataccat tgaattcgac tgacaaatct tagtgacagg aattatttcc
     8641 tgtatggtaa aataccagga tgaaaacaca gctaaccgcc atttcttcat ttactcttgt
     8701 ttgtcgatga ggaaatctca aacaatttaa ataccaagat ctgtataccc aatccgcatc
     8761 tgcacgtctc aagcctgtca gagatggcct tcgagacgtg gctttagcca ctcctcaggc
     8821 cgaacgtaac aaaatgtcat gttcctcgct gcttaaattc tacatagctc tctaacgagc
     8881 aatccgcagc aggtaaactt ttaacaggaa taaaaaaaaa atatcaatag aaccctagtt
     8941 ttcatcatag ctgcgcaaaa ccagatgtgt attgctgcct cctgccccat agctggaaac
     9001 accagccaaa tagtgttttt ttactgtagg gaaatcttga aattcagtgg cgatgtatat
     9061 aggtgagtct tccaacataa aatctggatt aggagtttca aaattaattg aaggaacaat
     9121 agccttattt ttgagcataa gtactgtttt gatgattgaa gccataccgg acacgatgcc
     9181 tgtatgaccc aggttactta tcaccgaact tattggacat ctatatgtct ttttaacgtc
     9241 tttgaacgct tttttcaggg cgtatatttc tgttgcatca cccagtttgg ttgaggatcc
     9301 atgagctgca atataatcca aatcatctgg ctcaacctct gcatttcgcc aagcagactg
     9361 caagcaagta tattggccat gaacccctgg tgccataaat ccttcttttg aagaattacc
     9421 atcattattc atggcggatc cgataatcac agcatggatt agatcatgat ctgcaatggc
     9481 atcttcaagt cgctttaaaa caactaaccc agcaccattt gagtataaag tgccgcatgc
     9541 tgaagcatca aatgaatggc aatggccgtc ggatgaaaat aagccattct cttcataaag
     9601 atacccaact tcctgaggta aatcgattaa acacgcacct gcaattgcca tatcacattg
     9661 atgactgttt aactctctac agctttggat gaccgcagtt aaagaagatg agcaagctgt
     9721 atataaatca atggagcttc cttgaaatcc aaattgataa gaaatataag gcgcgatgac
     9781 ttttgaataa tacctttcta tcgctttttg agtatcaatc gactggtagt aggcggctcc
     9841 caggaaatgc gcggctggtt gattatttgc gccaatataa acagctgtat tttttatgta
     9901 gttatttgaa tgatgttcct gacttctcat cgtactcaaa gcacgatgag ttaaatgcga
     9961 catcactttg ccttgcacac ccaacaattc aaaatttttg gtggtattct caaacggagc
    10021 agtatcgaat gactccaggt catctaatat ccctctgtag ggtttgtaat tgggattagc
    10081 gattaattgc tctgaaatac ctgaccgatg taattgttct tcggaaaatt tagttactgc
    10141 ttctttacca aacttcaata aatcccaata ttcatcaaga tcattggctc cgggaaattt
    10201 tacagccata ccaacaatag caattttgtt tgagtagtta ttgtcactca taatttatgg
    10261 ctccatttct ttggccgcac tcaacatgta ataaacacta accagacgac attggaaggg
    10321 ttcatagcat gaaacatagt ctacgttatt cctgcaacca gcttttaatt aagtataagt
    10381 cattttcctc aattattttt attactcaga tgttgcccaa gctaaatgaa agtcattaat
    10441 ttctctttgt ctatttttcc attttctttt attgggaatg cgcttagcca tagataatgc
    10501 tctggtacca tatagtctgg aagcctggtt tttaaaaacg atttaagttc agcgctgcca
    10561 atctttgttt gattattagc aattaggaac acgaccattc tataatcaag accctctact
    10621 ttctcacaga caacgacggc atcctggatg ttgggatgat ctaataaatg atgttgtatt
    10681 tctcctaatt caatacgtat accatgtaat ttgacctgat catccctgcg acctaaaaac
    10741 aataattcat gattttcatt ccaacggact atatctccag tacgatacat tttttctcca
    10801 ggcaagtagg gattgtctat aaacgatgtc cgagtaagcg ctggattatt taaataaccc
    10861 tgagccaatc caactccgga aatatatagc tctccaggtt cgaaaccggt tgagtttaac
    10921 tgctcatcta acacaaaaaa ttgagtattt tggataggct taccaattgg cacagtgcga
    10981 taatctttat gtttttcaca aataaaacag gaaacatcta ttgtagcctc tgtgggtccg
    11041 tataaattga ctaatattgt tttttgatct tgaagtaact tattaaatag attaacactt
    11101 ttggcatcca gtgcctctcc acttgaaaag acatacttta aagagcttaa gcgatttatc
    11161 gaaaaatcct gttcgatgta acttaaaaat attcgtaata cggagggtac aaaatgaata
    11221 acgtttattt gatattgctc aatcatttga ataaatagct tgatatcatg ttcttttcct
    11281 ggcggtaata gtactactcc ggcccccgca atagaccacc aacaggtttc ccaaactgag
    11341 atgtcaaagc aaatgtgtgt tttatgaaac actacatcat gagtttgcaa tggaaatgtg
    11401 ttttgcatcc atagaattcg atttaccaga gcctgatgtg aaatcatgac ccctttcggt
    11461 ttgccagtag tgcctgaagt atacatcaca taagctaaat cctgtttttc tcgaggcagg
    11521 tttaaattat ggcaatcttc atcgctcaca ttggcatgaa tgcttaatac ttgcaaccca
    11581 gctttttggg tcaataatga attatcacta acaattagaa atttgatttg gctgtcttca
    11641 aggatggttt gatttcttat gaccggagcc atgggatcta tcggtaaata agcacctcct
    11701 gcctttaata ccgcaaaaat tgccaccagc atttcaaaag aacgcgtgag ctgaatgccc
    11761 acaattccat ttaatggcag tccttttctt tgcagatacc gtgcgtattg gttgacttgt
    11821 ttattaaagg catcatacga aatttgacga tcttccagat aaaccgctgt attttctgga
    11881 tattgcttta ccttctcctc aaataaggag caaattgtcg tgtcctgatc aggagatctc
    11941 attgtttaat aatatcctct atttattgat gcaaagtttc gaaatgagta tctcgtgcat
    12001 ttgcgaagtt ccttcaatga tctccattac cttagcatct ctgtaatatc tctcaacagg
    12061 ataggtttca tcaaaaccag ctgcgccaaa cagctgcagt gcatggttag atacaaaaac
    12121 gaccattttt gaagccgcgt attttgctat taatgtttca gctatgtatg ctggattttt
    12181 tttttgccta agttctccgg cataaaaaca caattcccgg gcggctttaa cctgcaccac
    12241 catttcagtt atcatttttt gaatgagctg atgttcgtct aacctgcgat tgaattgttt
    12301 tctttcttga gtatagccaa gagccgcatc caggcaagct tgccctaaac caacacatcc
    12361 atacgctgtt gtataacgtc cttcatctaa agcagtttgg ataacatagg taaaaccgac
    12421 accaatacta cccagcagat ttgttttagg aattcggcaa ttatcaaaaa aaatctctgc
    12481 cagcatatta gagcgtaaac ctaaaaaatt agacatgggt gaaataatta aacctggcgt
    12541 gcctttttca actaaaaccg cagtaggttg accctggcaa tttgccaaaa ccaaaaacaa
    12601 atccgctatt tgccctaagg taatgtattt tttactgcca tttagaatga actcatcacc
    12661 tgcatccaac aactgagttt caacatgttc caaatcgctg cctatattag gttctgttaa
    12721 agctaatgca gctaaagttt ctcctgaggc aatccggggt aaccagattt gcttctgtgc
    12781 ttttgaacca aactgcaaaa gtgacttact caccatgccc aatactgtta ggatatttgc
    12841 caaagaacaa agctctttag caaaggattc atacatgata ccgatggtca attcatccca
    12901 ttgttgcccg ccaaattgtt tgggaatgca agcgcccaaa aaaccatttt gagccaacgt
    12961 ataaaaaaaa tcgcgcggaa tgttttgttc tttatcaaat ttttttgcat gagggtgaag
    13021 acaattttct atgaattgat taaagaactg ttgttttttc acttgtcgag gctgatagtt
    13081 gggcaaaatt cctctcctta gtggctgttt gcaattctac tcatattgat aaaatccttt
    13141 ttttgttttt tgtccaagaa gccctgcatc gaccatattt tttaataacc agcatggtcg
    13201 atactttgga tcgttcagct ctgaatagat tgtttctaaa gaatagagaa ccgtatccaa
    13261 cccaattaaa tcggcagtat gcagcggccc catcttatgc ccaaagcatt gtttgaatag
    13321 aacatcgatg tcttcgattg atgcaatgtt ttcctgtacc ataaaaatgg cttcgttaat
    13381 aaaaatcatc atggcacgat tactcacaaa accaacggag tccttaacaa cgatcatttt
    13441 tttatggact tgctcaagta aggttctggt tttttttatt gtcgattcgt cagtgtgata
    13501 tcccttaatc acttctacca taggcattat cgtcgctggg ttcataaaat gaacccctat
    13561 aacgcgttgg ggatgttcaa cgagcgatgc tattttagta ataggtatag aggaggtgtt
    13621 tacccccaaa atacaggttg ggttgccttg ttcatttaat acctgataca gtgctttttt
    13681 tagctcccaa ttctcagtga tattttctat aatatattcc gattccttaa gttcatctaa
    13741 ttgtgttgta aaagcaattg atgccagtat agtttcaata gaatgggtca atgctaaatt
    13801 ttgagttaat gccagatagt gtaaattttt agctatagtg gtctttgctt tatccagttg
    13861 agattggatg ttatcgatta aagtgaca
//
LOCUS       DACTJE010000078        13832 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.078, whole genome shotgun sequence.
ACCESSION   DACTJE010000078 DACTJE010000000
VERSION     DACTJE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13832
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.078"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..823)
                     /locus_tag="I9760_001760"
     CDS             complement(<1..823)
                     /locus_tag="I9760_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HAT5043528.1"
                     /translation="MQAEVFYKSCGDRIVPVHLISQSQWDEGIENLTPMERNCFSVRQ
                     FKGKLGDYCFILNADGVIEKAFIGSGTGNQESALANAALLLPPGNYQFQDKLSRESGV
                     VWALAQYRFDEYKKYELQPRVLIVEANDRDYILSLANSLFLVRDLINTPTCDMGPESL
                     AHVVEELAKTHKAYFKQWIGEELIKDNFPAIHAVGRASALTPRLLSLHWGNEKHPRVT
                     LIGKGVCFDSGGLDIKPSSGMRLMKKDMGGAAHVIGLAQWIMVQRLPIRLQVLIPA"
     gene            complement(828..2399)
                     /gene="murJ"
                     /locus_tag="I9760_001761"
     CDS             complement(828..2399)
                     /gene="murJ"
                     /locus_tag="I9760_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAT5043529.1"
                     /translation="MLTTDIMVPKKQSLIRSTSLVSLMTFISRIVGFARDMVLANFFG
                     AQAGMDAFFVAFRIPNFMRRLFAEGAFSQAFVPVLAEYQKTRSAEDVRTFIARISGYL
                     SSILTLVTVVGIVASPVIIFLFAPGFHHDSVRAELATQMLRITFPYLMLISLTAMAGA
                     ILNTYGYFGVPAFTPVLLNISMILAAVYLCPNLPQPVVGLAWGVLIAGIVQLLFQLPF
                     LYQRHLLIRPRVVRDDPGVNKVLKLMIPALFGVSIAQLNLMVDSIFASFLKVGSVSWL
                     YYTDRLTDFPLGVFGVAIATVILPHLSRKHTEQNINQYSRALDWGLRSILLIGIPAGL
                     GLSFFALPLIASCFAYGKFTAYDLLQTQKSLITLAMGVPAFMVVKVLASGFYARQDIS
                     TPVKVGAIAMVVNTLLCFVFVWHLAHAGLTLASALAGYVNCAILLFLLIKRGLFKPSP
                     GWLKYSMQLLIANFAIGVYLYFMSGTATYWLGFSPLMRLSLLLAHVFVTVVIYLLVLG
                     IIGLRPSHFRGQIKD"
     gene            2757..3023
                     /gene="rpsT"
                     /locus_tag="I9760_001762"
     CDS             2757..3023
                     /gene="rpsT"
                     /locus_tag="I9760_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAT5043530.1"
                     /translation="MANIKSAIKRARQNVKLRQHNASARSMYRTYIKNVLKAVESGDQ
                     EAARAAYTKAQPVIDKAANKGLIHKNKAARIKGRLVARLKAMAA"
     gene            complement(3084..4295)
                     /locus_tag="I9760_001763"
     CDS             complement(3084..4295)
                     /locus_tag="I9760_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2637 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043531.1"
                     /translation="MFLTINDLQSQIQLAKKRLHVLAKNWLDTQKEAINSKDKANWLN
                     AFQASTKEAMQEQLKATGFPLIRNAILVNQSEDELPQHRLEQSSSLIHSENFPEQFNS
                     RTVPRVIYVCHSGENNQVIKRLCQLTTLMEQLHTLEELTFEVQTNVHFRGLNLAYRMA
                     RSINKEWRDVEKGRETVEYYAERLQIAEKQYKLASDFCHECLGIYKEQLDARIMYFNE
                     SLNFFVECLAATFSSVKDDRYWLTKFLLPSLPWHSMLNEVATCLFTTNGFTNPTIDSI
                     EVNRSHLKAITEQFAKLMMAPFCSLEKELQGKIEKAENEQKIARENVYAMSSELDIVI
                     HMQSQRKLAALLFPAQPLSMLGFFQGVAAAMGRDYFYSTFIKGNQSQEPRITDDEKPL
                     IEASNYSNGLH"
     gene            complement(4647..6029)
                     /gene="mavV"
                     /locus_tag="I9760_001764"
     CDS             complement(4647..6029)
                     /gene="mavV"
                     /locus_tag="I9760_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavV"
                     /protein_id="HAT5043532.1"
                     /translation="MKYAVTFCTTDQEVGSNPLWHSCLLLSKMDETSKLLEVVDNWGF
                     YGLPTTNRNSSWLDQLKIKMGLDVDLIGNHGMLRHEELRFLDQGCGLRGVTFELTKDD
                     FELLQKKCLQMATDQETAINEFVETQGIKGKPPEKTRIYPHEQFSRLIYATEKIKAEQ
                     QGREARLKPFELRLSWGLFGPSLALSQNCKTQALSLLSYVLSKEQIERLTEYGEHPTV
                     PRRSGPMESIFLHSTGPLREHRKSSGDVVHYRDLKDPGVKLYWTVPPQELEALSEETI
                     KLFQIDGEYCEEVKAIVRKLQRLEWLLINSNLPEKYRPYKDELINKVVGCYKAFSFIE
                     PKQEEPKLGGWYGWALSLFSAPRSAEERTLQGKIKKAKSLFNSIYMAIVDNYQIDDNA
                     PMENKNILPYEETGENRFENMVEAVASYLSRKDKINLCKIIGRTYCEDHVELEDNEGK
                     IALSPQPLAT"
     gene            complement(6437..10039)
                     /locus_tag="I9760_001765"
     CDS             complement(6437..10039)
                     /locus_tag="I9760_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAT5043533.1"
                     /translation="MKSLVPWVCVLVASAGQQAFANDAFNAYRLGNYNEAAEPLIIKS
                     GQDAVAEYYLGRLYLYGYGQLKNNRVAMRYFTKSAEKGYLPAVLLMAKYSLLHEKNPE
                     EAARWFKQAAAVGDVSAQMYMAAAYLYGVGVKKNTDIARRYYIDAAKNGNPIAQYTLA
                     EYFLESKHAGNKKLGIIWLNKAAENGDPKALTELGRFYVAGQMVAKDTAKGVELLNKA
                     ASEQYTPAMLELGKLALMQNQYDEAIQWLNKAEEQSTEATLELAHIYLQDKSPVYDPK
                     TGFLLVLKAAQNGSVQAQKELANLYQKGIGVAADEELSKQWTLKAAENEKKNAEPPVL
                     AQAALWLSNDRTDKLEETAYQMNGIFSPWNNPSALRNNSYNQAPKLETISRQDIFKPQ
                     FELTQPNSIPISNYYDALISKQVEFQSNQWAYPTYRLNPFVEALERANSPVVQKLNLP
                     VPYADANYDDSEVGSFDLMDIWTGDWKRTVNYMSVVNYLYFRAILGNAQTQFEIGQLF
                     QYGIGLMQDDASAIIFYENAAEQKHLGAEYNLGILYLKRGKDENDYQQALNWLTDSAF
                     KGNKRAQYVLARILRQGIVGPDGKEYIKANDEQAMAMLYLSAANDYGPAEYELAEYLA
                     RDYNNGLSVDVRKQKIALIRKLYQGAVKNKVAEALLPLAFYNAMDDNRQKQEQAFKVA
                     EEQAETGNENAALLLGMLYDRGIGITADPAKAMYWYQQAGQNPVSQFILGTYITEGKG
                     VAQDKEKGLDLLKQSADSQFSYADFNLAVLKQQSGEDFLPNLIESYKLGNSHAGIVLA
                     DYYLSQSSVPEQMNQAKEIYKGLADKGDQYAQLKLAYMLQKGLGAAPDLAGAQHWYTA
                     SAEQGNPEAQYLLGQFYQLGETGEPDYNLAKHWYQKAAKHLSKADVALGFIYETVDDN
                     YAQALKAYENAAAKGDMLGAYNLALMYEYGKGVPVNYSKALSLFKEASEKGAPEAMSQ
                     LAGMYFYGLGQPRNEQQALVWYKKAASLGNGNALYALGLLSETGVGVKLDFPDALRYY
                     QDASDKGNEKAMLALARMYHYGLGVEKDHKRSASIYQKLAQKQNAYAQYQLGTYYIEG
                     TAGERLPEKGRELLQQASENGSLQARRVLQRLDAQTQGKVSYVEPVLFNNVPKFDGQS
                     ADMIYFDALNEWNHGEEVLSRMILQRLVTQYPNFIPAKRAYEQLNQAKLMNNLG"
     gene            complement(10066..10632)
                     /locus_tag="I9760_001766"
     CDS             complement(10066..10632)
                     /locus_tag="I9760_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase IV"
                     /protein_id="HAT5043534.1"
                     /translation="MNLKSRSSITAIVVILLTGALNMSAYAAKSNKTATAEEKAEKKD
                     NGKFPLGCTAVGYQQNLKVLTLLPGKEGALQSMYFIYNTLPETVSLYQMHDKESLYSY
                     WLNHSIRGNSWAVLATGEKEVKFICTLGDGKKSYGKILDCADVIKVCEYVNVKFGLNN
                     KGNFWIVDSNTRNGAVSQVVHYGIIPGV"
     gene            complement(10629..11351)
                     /locus_tag="I9760_001767"
     CDS             complement(10629..11351)
                     /locus_tag="I9760_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAT5043535.1"
                     /translation="MKKILLTTFMMSMVTVLNAGHFYGTALCAYPQYECIKVTGGQSW
                     EKLFPDPVQRDIVQRVNRTYNYLWSGREIAVPKNLANINIFDVSPFPLKIQSENEKQI
                     IVDQEKLAWGAYDAQGNLVWWGPISSGSDKCSDSNKVCRTLTGIFRVFSKENVRCTSD
                     VFPIGRGGAKMPYCMYFHKGFALHGSDDIPGVRASHGCVRMFIQDAKWLNENFVELSS
                     ERNNFMGTKVIVRPLNDSENKK"
     gene            11524..>13832
                     /locus_tag="I9760_001768"
     CDS             11524..>13832
                     /locus_tag="I9760_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT5043536.1"
                     /translation="MSPLKSKIKTKSFNKLTKTQDITNTIERLFANSQDSIIVFNQQG
                     KIIAINEKAAALFKKSPDKLIDKPIWKLFKLNSFAIKRQFIQAKRCFLLANEGIAQQF
                     TWLESKAQKPVLAYHILFNKTEIEDTSIIFTKLTDILKSKTIEWILWSLTKISNHQEI
                     NEIIDQILQLTSDVFAADYTSVSFINNQQIARTISYYKFGKKEENISFSLINTPCAKV
                     IQKKAICYFNDVQKQFPNDKLLKRMNINTYLAGPITNPQDEVIGIMTILCKRKVEFDT
                     LNNTLFGLFLSRINSEIERLINLRKLEFLASIPQQNPNPIIRILLNGDVVFTNDEGKI
                     ILNYWIKLFKGLPVELLKEAQRIQKSNQSTRIEMEIEDKIYLFTLVWIDEFKQINIYG
                     TDITQLKNTQNAMLNLTRRDTLTQIANRQYFEEKLIEKIYEHHLKEKSLALLLIDLDN
                     FKIVNDTLGHPTGDKLLMAVTNRMTRCLRANDFIARLGGDEFIVLLDQCNTDTAVKVA
                     EKIINVLGKPFQFDEYHMTITASIGIAIYPETSEIASDLLKYADIAMYQSKKTGKNKC
                     TVFSKNLHYKENQRNEILKKDIELAALKRELYIDYQPQIDLSDNTIVGIEALLRWSHP
                     TLGLILPTEFIPIAEQTGCIHTISQWFIEQTLLDYSQFQQLSSNIKLSINVSLSQLND
                     TRFTKILCDSLLQNNLSKSKIILDISERMIAPHFIQISKKLKQIHHIGINISLDNFCC
                     PQISLPELLSLPLDYLKVDQRLLTGIEGK"
BASE COUNT         4354 a         2965 c         2323 g         4190 t
ORIGIN      
        1 cagcggggat taatacctgt aatcgaatcg gcaaacgctg aaccattatc cattgggcta
       61 aacctatgac gtgtgcggca ccgcccatat cttttttcat caaacgcatg cctgatgaag
      121 gtttgatatc aagcccacca ctatcaaaac aaacaccttt tccaatcaaa gttacacgtg
      181 gatgtttctc atttccccaa tgcaaggata ataaacgagg cgttaaagca gaagctcttc
      241 caaccgcatg aatagcagga aaattatcct ttatcaattc ctctccaatc cattgcttaa
      301 agtaagcctt atgggtttta gccaattctt cgacaacatg agctagcgat tctggcccca
      361 tatcgcaggt tggcgtgtta atgagatccc gcacgagaaa taaagaattc gccaaggata
      421 aaatataatc tctatcattt gcctcaacaa ttaaaactcg aggctgtaac tcgtactttt
      481 tatactcatc aaatcgatat tgtgctaatg cccaaactac gccagactca cgacttaact
      541 tatcttgaaa ttgatagttg cctggaggca ataaaagtgc ggcattggcc aaagcacttt
      601 cctgattacc agttcccgat ccaataaaag ctttttcaat cacgccatcc gcattgagga
      661 taaaacaata atcgcctaat ttgcccttaa actggcgtac agaaaaacaa tttcgttcca
      721 tgggggttag attttcaatt ccttcgtccc attgcgattg agaaatgaga tggacaggaa
      781 ctatcctatc gccacaactt ttgtaaaata cttctgcttg catcttttta atcctttatc
      841 tggccacgga agtggctagg cctaagccct attataccta aaactaataa atagatgact
      901 acggtgacga acacatgagc taataacaag ctcaaacgca tcaagggaga gaaaccaagc
      961 cagtaagtcg ctgttccact cataaaatat aaataaaccc caatggcaaa attggctatc
     1021 aacaactgca tactgtattt cagccatccg ggagatggtt tgaacaatcc ccttttaatc
     1081 aagagaaaca acaaaatagc acaattcaca taaccagcta gtgccgaagc caacgtcaag
     1141 ccagcatgag ccaagtgcca gacaaataca aaacataaaa gagtattaac taccatggct
     1201 atggcgccca cttttacagg ggtgctgatg tcctggcgag cataaaaacc ggaagccaac
     1261 actttaacca ccatgaaggc aggaactccc atggcaagag taatcaggct tttttgagtt
     1321 tgtaataaat cataggcagt aaatttgcca taagcaaaac aactggcaat taaaggcaag
     1381 gcaaaaaagg acaatcccaa accggctgga attcctatta ataaaatcga acgcagcccc
     1441 caatccaaag cgcgagaata ttgattaata ttttgttctg tgtgttttct ggaaaggtgg
     1501 ggcaagataa ccgtcgcaat ggcgacccca aaaactccca aaggaaaatc agttagtctg
     1561 tctgtatagt ataaccaaga cacactccct acttttagaa aagaagcaaa aatactatct
     1621 accatcaagt taagttgcgc aattgaaaca ccgaataagg ccgggatcat taacttaagt
     1681 actttattga ctcctggatc atcccgaaca actctcggcc tgataagcaa atgtcgttga
     1741 tacaaaaatg gtaattgaaa aagcaattgc acgataccgg caatcaacac accccatgcc
     1801 aaaccaacaa cgggttgtgg caaattaggg cacaaataca ccgcagcaag tatcatactg
     1861 atattaagca aaacgggagt aaatgctgga accccgaaat aaccataggt atttaatatt
     1921 gctcctgcca ttgctgttaa ggaaataagc attaaatagg gaaaagtgat acgaagcatc
     1981 tgagttgcca attcagcccg aacgctgtca tgatggaaac ctggcgcgaa taaaaaaata
     2041 ataacaggcg atgctacaat tcccactaca gtgaccagag ttaatatgga actcaaatag
     2101 cctgaaatcc gagcgatgaa agtacgaacg tcttcagcag aacgcgtttt ttgatattca
     2161 gccaataccg ggacaaaagc ttgagaaaaa gcgccttccg caaaaaggcg ccgcataaaa
     2221 tttggtatcc tgaaagcaac aaaaaaagca tccatgccag cttgagctcc aaaaaaattg
     2281 gcaagaacca tatctcgcgc aaacccgact atccttgaaa taaatgtcat taatgaaaca
     2341 agggaagtgg atcgtatcaa gctttgtttt ttaggtacca taatatccgt tgtcagcatg
     2401 atgttttaaa tagataaaaa ttttctttat gatatactta aactggcgtg agttcgatat
     2461 aaaaagcatg gaaatacctc ttatatctgg ttaaactggc attatccagt aaaacacaac
     2521 acatcagtat acaggttcca ctgcaaatga agtcgatcat ggtatcaaac cagtaagtga
     2581 atcgcctgaa atattggttt tacaggattt atccggataa taaaacattt caacatcaac
     2641 ctgcgttaaa acaaatacat aaatacaaaa ggttcaaagc aggtctattg acaaattcta
     2701 actcattgtg caagatctac atcttttgca acatctgatt gagtggagat ttttaagtgg
     2761 caaatatcaa atcagcgatt aagcgtgcac gtcaaaacgt caaattgcgt caacataacg
     2821 ccagcgcgcg ttccatgtac cgcacctaca ttaaaaacgt gctgaaagcc gtagagtcag
     2881 gagatcaaga agctgcgcgt gctgcttata ccaaagcaca accagtgata gacaaagccg
     2941 ctaataaagg cttaattcat aaaaacaaag cagcacgtat taaaggccgt ctggttgcac
     3001 gtcttaaagc tatggctgcc taatcgagag tttccgtcgc tctaagttcg aaactaccga
     3061 accgtaattc ccggttcggt tctctagtgt agtccgttag aataattgct tgcctcgatg
     3121 agaggttttt catcatctgt tatcctgggt tcttggcttt gattcccttt gataaaagtt
     3181 gaataaaaat aatctctacc cattgctgct gcaaccccct ggaaaaagcc taacatactt
     3241 aatggttgag cagggaacag caatgcggct aattttcttt gtgactgcat gtgtatcact
     3301 atatccaatt ccgaggacat ggcatacaca ttttctctgg ctattttttg ctcattctct
     3361 gctttttcta ttttgccctg taactctttt tccaaagaac aaaaaggcgc catcatcaat
     3421 ttggcaaatt gttctgtaat tgcttttaaa tgtgagcgat tcacttcaat gctgtcgatt
     3481 gttgggttgg taaagccatt tgtagtaaag aggcatgtag ctacctcatt aagcatgcta
     3541 tgccaaggta aagagggtag taaaaatttt gttagccaat acctgtcgtc ttttacagag
     3601 gaaaaagtgg cagccaggca ctcaacaaag aaatttaatg attcattaaa gtacattatc
     3661 cgagcatcca attgctcctt ataaatccct aaacactcat gacaaaaatc cgatgccaat
     3721 ttatattgtt tttctgctat ctgcagacgc tccgcataat actctacagt ttcacgcccc
     3781 ttttcgacat cgcgccactc cttattgata ctgcgtgcca tgcgataagc caaatttaag
     3841 cctctaaaat gaacattagt ttggacttca aaggttaact cctccaatgt atgcaattgt
     3901 tccattaagg tagtaagttg gcataatctc ttgattactt gattattttc tcctgagtgg
     3961 catacataaa tcactctggg cactgttctt gagttgaatt gctcggggaa attttctgag
     4021 tggatcaaag acgaggattg ctccagacga tgctgcggaa gttcatcctc cgattgattg
     4081 accaatattg cattgcggat taggggaaaa cctgtggctt ttaattgctc ctgcattgct
     4141 tcttttgtcg aggcttgaaa tgcattgagc caatttgcct tatcttttga attgattgct
     4201 tccttttgag tatccagcca gtttttggct aaaacatgca gtctcttttt agccaattga
     4261 atttggcttt gtaaatcatt tatggttaaa aacatccttt tatgctcact tgcttgcttg
     4321 agtgtcctta atctatcact ctaaaatgca tacttcaatc aaaaaaattc attcataacc
     4381 ggatatttta atacaaattg tttattaata aacaacatgg attatgtaaa aacaaaggtc
     4441 aaggcaaaaa acatttttaa tgaggtggtt tttataaact aaatgactga attaaaacca
     4501 ttttttaacg aagagattaa gagatttcat aaatcctttt ttatttgata ttaattccta
     4561 aaaagggttg gtctttatca gtccctcaac accaacccaa gctggaatca caaaaagctc
     4621 atgaactgga atctattcca caacctctac gttgccaaag gttgtggaga taaagcaatt
     4681 ttcccctcat tgtcttctag ctcgacatga tcctcgcaat aggtgcgtcc gataatttta
     4741 caaagattaa ttttatcctt ccttgataag taagaagcta ctgcttccac catattttca
     4801 aatctgtttt cgccagtttc ttcataggga aggatattct tattttccat tggagcatta
     4861 tcgtcaattt gataattatc cacaatggcc atataaatgc tgttaaataa cgattttgcc
     4921 tttttaattt tgccttgtaa ggttctttct tccgcacttc ttggtgctga aaataaagaa
     4981 agcgcccagc cataccaacc tccaagttta ggttcttctt gcttaggctc aataaaagaa
     5041 aaagctttat aacatccaac taccttattg attaattcat ctttataagg ccgatatttt
     5101 tcaggtaaat tgctattgat taataaccac tccaggcgct gcagttttct gacaattgct
     5161 ttcacttctt cgcaatattc cccatctatt tgaaataatt tgattgtctc ttcagataat
     5221 gcttcaagtt cttgcggagg aactgtccaa taaagcttta ctccaggatc ctttaaatca
     5281 cgatagtgca caacgtctcc tgaagatttc ctatgctccc gtaaaggacc cgtactatgt
     5341 aaaaaaattg actccattgg cccactacgt ctgggcactg tgggatgttc accatattct
     5401 gtaaggcgtt caatttgctc ctttgataat acataagaca gcaatgataa agcttgtgtt
     5461 ttacagtttt gtgaaagagc taaactaggc ccaaacaatc cccaggacaa acgcaactca
     5521 aaaggtttta agcgagcttc ccgcccttgt tgttccgctt taattttttc agtcgcataa
     5581 atcaatcttg aaaattgttc gtgaggataa attctggttt tttcaggtgg tttcccctta
     5641 atgccttgcg tttcgacaaa ctcatttatc gctgtttcct gatcagttgc catctgcaaa
     5701 cacttttttt gtaataactc aaaatcgtct ttggttaact caaaggtaac cccgcgcaaa
     5761 ccacatccct gatcgagaaa tctgagctct tcatgccgca acattccatg attgccaatc
     5821 aaatcaacat ccagccccat ttttattttc aattgatcta accaagaact attacgattg
     5881 gttgtcggta atccataaaa tccccaatta tcaacaacct ccagcaattt actggtttcg
     5941 tccatttttg aaagcaatag acaactgtgc cacagtggat tactccctac ctcctgatct
     6001 gtagtacaaa atgtaacagc atatttcata tcaccaccct catcgatttg catctaatta
     6061 tttgaccttg attaaataga ccttttgcct aaagccgatt ctttcatttt attgctgctt
     6121 tgctaacatt cctgcattgc tcattaactg cattataaac tccgctcctc aaaacgtttg
     6181 caccttgccc taaaaaataa tcggcttagg cataaagcct aactaacaat ttaaatctct
     6241 aaaagattat ttttaaaatt acaataaggc atggcttcac tttcgtaaat agccaagatg
     6301 agcgcaaccc ttcatgtgtt taatataatc gaggcaacaa atgcgggctg ggccttgtgg
     6361 cccagaaaaa tcggataatt gattagcaaa attgaaacat catctgttaa atagctcaaa
     6421 gagccgagca atgatcttaa cccaagttat tcattaattt ggcttgattc aactgctcat
     6481 aagctcgctt tgcagggata aaattgggat attgcgtcac cagacgttgc aagatcattc
     6541 tggacaaaac ctcctcccca tgattccatt catttaatgc atcaaaataa atcatatcag
     6601 cgctttggcc atcaaatttg ggaacattat taaataacac gggctcgaca taacttactt
     6661 taccctgggt ttgtgcatct aatctttgca atactctacg ggcctgtaaa cttccgtttt
     6721 cactcgcttg ttgtaatagc tcacggcctt tttcgggtag tcgttcgcct gccgttccct
     6781 caatataata agtacctaat tgatattgag cataagcatt ttgcttttgt gccaattttt
     6841 gataaatact cgctgatctt ttatgatcct tttcgacacc aagaccataa tgatacattc
     6901 ttgccaaagc cagcattgct ttttcattac ctttatcaga tgcatcctgg taataccgta
     6961 aagcatctgg aaaatccaat ttaacaccaa caccagtttc ggataataaa ccgagggcat
     7021 acaacgcatt cccattacct aagcttgcag ctttcttata ccagaccaaa gcttgttgtt
     7081 cgtttcttgg ttgccccaaa ccataaaaat acattccagc caattgactc atcgcttcag
     7141 gagcaccttt ctctgatgct tctttaaaca aagataacgc tttggagtaa ttgacgggca
     7201 cgcccttgcc atactcatac attaaagcca aattataagc gcctagcata tctccttttg
     7261 ctgcagcatt ttcataggct tttagtgctt gagcataatt atcatctacc gtttcataaa
     7321 taaaacctaa tgctacatcg gctttagata aatgtttcgc tgctttttga taccagtgtt
     7381 tggccaaatt ataatcaggt tctcctgttt cacctagttg ataaaattgg cctaataagt
     7441 attgtgcctc aggattgcct tgttctgccg aagcagtata ccaatgttgc gcgccagcaa
     7501 gatccggcgc ggcacccaat cctttttgta gcatataagc caattttaat tgtgcatatt
     7561 gatcgccttt atcagctaag cccttataaa tttccttagc ttgattcatt tgttcaggaa
     7621 cactgctttg agataaataa taatctgcca atactatccc cgcatgacta ttccctaatt
     7681 tataagattc aattaaattg ggtaaaaagt cttcccctga ttgttgcttg agtactgcca
     7741 gattaaagtc ggcataggaa aattgggagt ctgctgattg cttcagcaaa tccaatcctt
     7801 tttccttatc ctgtgctacc ccttttcctt ctgtgatgta agtacccaat ataaactgac
     7861 ttacgggatt ttgtccggct tgttgatacc aatacatggc cttggctgga tcagctgtta
     7921 ttcctatacc acggtcatag agcattccga gtaaaagagc ggcattctca ttacctgttt
     7981 cagcttgctc ttcggctact ttaaatgctt gttcctgctt ttgcctgtta tcatccatcg
     8041 cattataaaa tgccaatggc aacaatgcct ctgccacttt attcttcaca gcaccttggt
     8101 ataactttct tattaaggca attttttgtt ttctgacatc tacacttaaa ccattgttgt
     8161 agtctcttgc caaatattct gccaactcat attctgccgg cccataatca ttggcagctg
     8221 aaagataaag catggccata gcttgctcat catttgcttt aatatactct tttccatcag
     8281 gaccaacgat cccctggcgc aatatccttg ccaatacgta ttgtgctctc ttattccctt
     8341 tgaatgcgga atcagtcagc caatttaatg cttgttggta atcattttcg tctttaccgc
     8401 gtttcagata aagtattcct aaattatatt cagcacccaa gtgcttttgt tctgcggcat
     8461 tctcataaaa aataatagcc gaagcatcat cttgcattaa accaatccca tattgaaaca
     8521 attgaccaat ttcaaattga gtttgagcat ttccaagtat tgccctgaaa tacaaataat
     8581 tgaccacaga catataattg acagtccgtt tccaatcccc tgtccaaatg tccatcaaat
     8641 caaaactgcc aacttcgcta tcatcataat tcgcgtcagc ataaggaact ggcaagttga
     8701 gcttttgtac aacaggacta ttggctctct ctagagcttc tacaaatggg ttaagtctgt
     8761 aagttggata agcccattga tttgattgaa actccacttg tttactgatt aaagcatcat
     8821 agtaattgct tataggaata ctattaggct gagtcaactc aaattgtggt ttgaaaatat
     8881 cctgacgaga gatggtttca agcttaggtg cttgattata gctattattt ctcagggcag
     8941 acggattatt ccatgggcta aatataccat tcatctggta agccgtctct tccaatttat
     9001 ccgttctgtc attacttagc cataatgcag cttgggcaag aacaggaggc tctgcatttt
     9061 tcttctcgtt ttctgctgct ttaagagtcc attgtttaga caattcttca tcagccgcta
     9121 cgccaatccc tttttgatat aaattggcta attctttttg ggcttgaaca gaaccgttct
     9181 gagctgcttt tagtaccaat aaaaaacctg tttttggatc ataaactgga cttttatctt
     9241 gtaaataaat atgggctaac tcaagagtag cttctgtact ctgttcctcg gccttattaa
     9301 gccattgaat agcctcatcg tattgatttt gcattaaagc caatttaccc aactcaagca
     9361 tggctggagt atattgttct gaggctgctt tatttaataa ttcgacgccc ttggcagtat
     9421 ccttggcaac catttgtccg gcgacataaa atctgcccaa ttctgttaaa gcttttggat
     9481 ctccattttc tgctgcttta ttcagccaaa tgatccctaa ttttttatta cccgcatgct
     9541 tactctctaa gaaatactct gctaaggtgt attgagcaat aggattacca ttttttgctg
     9601 catcaatata ataacgtctt gcgatatctg tatttttctt gacgccaaca ccatataaat
     9661 aagctgccgc catatacatt tgtgcgctca catctcccac cgcagctgct tgcttaaacc
     9721 aacgagcagc ttcttccgga tttttttcat ggagcaaact gtatttggcc atgagcaaga
     9781 cagcaggtaa atagcctttt tccgctgatt tggtaaagta gcgcatcgca acacgattgt
     9841 tttttaactg cccatatcca tagagataaa gtcgccctaa atagtattcg gcaacagcat
     9901 cctgacctga tttaatgatt aggggctcag ctgcttcatt ataattgcct aatctataag
     9961 cattgaacgc atcattggca aaagcctgtt ggccagctga cgcgactaac acacacaccc
    10021 aaggtactag tgatttcatg aagattcccc tcttttcatg gccatttata caccgggtat
    10081 aatgccataa tgcaccactt gactaactgc cccatttctc gtattgctgt ctactatcca
    10141 aaaattacct ttattattca aaccaaattt gacattaaca tattcgcata ctttaatcac
    10201 atcagcacaa tccagtattt ttccatagga ctttttgcca tcacccaagg tacagatgaa
    10261 cttcacttcc ttttcacctg tggccaaaac agcccaggaa ttccctctga tagagtgatt
    10321 tagccaatag gaatataaac tttccttatc atgcatctga tataagctaa cggtttctgg
    10381 caatgtatta taaataaaat acatcgactg caaggctcct tccttccctg gaagaagagt
    10441 caataccttt aaattttgct gatagccaac agcagtacaa ccgagaggaa attttccgtt
    10501 atcttttttc tccgccttct cttcggcggt tgctgtttta ttggatttgg cggcatatgc
    10561 cgacatattt agtgcaccag ttaatagaat tacaacgatt gcagttatcg agcttcggct
    10621 ttttaggttc attttttatt ctcactgtca tttaaaggac gtacaatcac cttagtcccc
    10681 ataaaattgt tacgctcact ggacaactct acaaaatttt cgtttaacca tttggcatcc
    10741 tggataaaca tacgcacaca accatggcta gcccttaccc ccggaatatc atcagaacca
    10801 tgtaaagcaa accctttatg aaaatacatg caataaggca ttttggcacc gcctctgcct
    10861 ataggaaaaa catctgaagt acaacgaaca ttttccttgc tgaatacacg gaaaattcct
    10921 gtcaaagtac gacagacttt attggaatca gagcatttat cacttccaga agaaataggt
    10981 ccccaccata ctaaattgcc ctgagcatca taggcgcccc aagccagctt ctcttgatcc
    11041 acgataattt gtttttcatt ttcactctga attttaagtg ggaaaggaga gacatcaaaa
    11101 atattgatgt tggctaaatt ctttggtaca gcgatttctc tgcctgacca aaggtagtta
    11161 taagtacgat tgactctttg cacaatgtct ctttggacag gatcagggaa taatttttcc
    11221 caactttgtc ccccggttac cttaatacat tcatattgag gataagcaca taatgccgtt
    11281 ccataaaaat gcccagcgtt aagtacagtc accattgaca tcatgaacgt tgttaatagt
    11341 atttttttca tggtgccgcc cccttatgcc tgatttttat aattttatgc aaaatttgtg
    11401 ctaaaaatga aatgccactc tttttatctt ttttcggtta tttttaattt aagtttagac
    11461 ccatacaaaa aaaaattgaa attattgcta tatttaaatt agcattgact gggttgaaca
    11521 ctcatgagtc ctttaaaaag taaaattaaa acaaagtctt tcaacaaatt aacaaaaact
    11581 caagacatta ccaacacgat tgaaagatta tttgcaaact ctcaagacag catcattgtt
    11641 tttaatcaac aaggcaaaat tattgctatt aatgaaaaag ctgctgcttt atttaaaaaa
    11701 agccctgaca aactaattga caaaccgatt tggaaactat ttaagttaaa tagcttcgct
    11761 attaagaggc aatttattca agcaaagagg tgttttctct tagccaatga agggatagcc
    11821 cagcaattca cttggcttga aagcaaagca caaaaaccag tacttgcata ccatatcctg
    11881 ttcaataaaa cagaaataga agacacatca atcattttta ccaaacttac cgatattcta
    11941 aaatcgaaaa ccatagaatg gattttatgg tctttaacta aaataagtaa ccaccaggaa
    12001 atcaatgaaa ttattgatca aattctacaa ttaaccagtg atgtttttgc agcggattat
    12061 acttctgtat ctttcataaa caatcaacaa attgcaagga caatcagcta ctataaattt
    12121 ggaaagaaag aagagaacat tagtttttct ttaatcaata caccctgcgc taaagtaatc
    12181 cagaaaaaag ctatctgtta ttttaatgac gttcaaaagc aatttccaaa cgataaatta
    12241 ttgaaaagga tgaacatcaa tacttatctc gcaggcccaa ttacaaatcc ccaggatgag
    12301 gtgattggca taatgactat cctgtgtaaa cgcaaagtcg aatttgacac actgaataat
    12361 accttgtttg gtttattttt aagccgaatc aattcggaga ttgagagact gataaacctt
    12421 agaaaattag agtttctggc gagtataccc cagcaaaacc ctaatccaat cataaggatt
    12481 cttctcaatg gggatgtcgt atttactaac gatgaaggga aaatcatttt aaactactgg
    12541 ataaaattat ttaagggact tccagtagag ttactaaaag aagcacaaag aattcagaaa
    12601 tccaatcaaa gcacaagaat tgaaatggaa attgaggata aaatctactt attcacactt
    12661 gtctggattg acgaatttaa acagatcaat atttacggga ctgatataac ccagctcaag
    12721 aatacgcaaa acgctatgtt aaatctaacc cgcagagata ctttaactca aatcgcaaat
    12781 cgacaatatt ttgaagaaaa actaattgaa aaaatatatg agcatcatct gaaagaaaaa
    12841 agcctggcat tattattaat cgatttagat aactttaaaa ttgttaatga tacattaggg
    12901 catccaacag gagacaaact actgatggct gttaccaata gaatgacccg atgcctaaga
    12961 gcaaatgatt ttattgcccg ccttggagga gatgagttca tcgtccttct tgatcaatgc
    13021 aataccgata ccgcagttaa ggttgcggaa aagattatta acgtgctcgg caaacctttc
    13081 caatttgatg aatatcatat gacaatcaca gccagtattg gcattgccat ctatcctgaa
    13141 acaagtgaaa tagccagcga cttactaaag tatgccgata ttgcaatgta ccaatccaaa
    13201 aaaactggta aaaacaagtg cactgtgttt tcaaaaaact tacactataa ggaaaatcaa
    13261 agaaatgaaa ttctcaaaaa agacatagaa ttagcggcat taaaaagaga gctctatata
    13321 gactaccaac cccaaattga cttatccgat aacacaatag ttggaattga agctttatta
    13381 cgatggtcac accctactct gggtttaata ttacctactg agtttattcc tatagcagag
    13441 caaacagggt gtatacatac catcagtcaa tggttcatag aacagacatt acttgattac
    13501 tcccagtttc agcaattaag ttcaaacatc aaattgtcga taaatgtttc cttgagtcaa
    13561 ttaaatgaca cgcgatttac taaaatattg tgtgatagtt tattgcaaaa taatttaagc
    13621 aagagcaaga tcattttaga tatatcagaa cgtatgattg ccccacactt tattcaaata
    13681 tcaaaaaaat taaaacaaat acatcatatt ggaattaata taagcttgga taatttttgc
    13741 tgcccacaaa tttcactccc tgaattgctg tcattaccac ttgattacct caaagtagat
    13801 caacgattat taacaggcat agagggaaaa at
//
LOCUS       DACTJE010000079        13826 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.079, whole genome shotgun sequence.
ACCESSION   DACTJE010000079 DACTJE010000000
VERSION     DACTJE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13826
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.079"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..85
                     /locus_tag="I9760_001769"
     CDS             <1..85
                     /locus_tag="I9760_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-pyrone-4,6-dicarboxylate hydrolase"
                     /protein_id="HAT5043537.1"
                     /translation="QDLFDEKLTEKILYRNARNFYSKNSSE"
     gene            complement(89..1894)
                     /locus_tag="I9760_001770"
     CDS             complement(89..1894)
                     /locus_tag="I9760_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HAT5043538.1"
                     /translation="MFGFVSDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHN
                     PSISRWYRSRNNASMKQRIIDSAYALAKNLPGVNQIIEKELNKELSSTREKLRIQRSG
                     MTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVTNDSPEREFLVERGDGKDSGALY
                     AIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKECYGLLTH
                     GGTTSIIEAMAAYVIRARAKGIDYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTG
                     AVNPKVMSSYITRNTAVIVGSAPSFMNGIHDPVSELGQLAKKKNVPFHVDACLGGFLT
                     AFLDTSSEPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAALNWSGGL
                     YATPGILDGSTSGARVAEVYATLSYYGKNKYQEIAKSIITLRNAIQKELTTLLEEGNG
                     LTSEDIYVYGNPQWSILGFRSNTCNAHFIADELEKRGWKLNLLQNPDGFHLCLTHVHT
                     LVKGFETQFIKDLREAVIDVKNYPPGKKPSGNVKVYGAVGMMPIELQREICKQYQKAR
                     LNYTSASPGSLRIFTNVPVEEDEGLRNRKTEKYKV"
     gene            2132..3238
                     /locus_tag="I9760_001771"
     CDS             2132..3238
                     /locus_tag="I9760_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAT5043539.1"
                     /translation="MKKKLALVMRLLAVILIPVLMSCNSESNKPVVHTLAEVDVALPV
                     KKNIVEWDEYTGRFQAVEEVDVRARVTGYLEAIKFQDGQMVKKGDVLFVIDQRPFKYA
                     LARAEAQYALAKAQYERAIKLQKEKFISAEVIDQRFQDVQVAETRVQEAKLNLEFTEV
                     KSPISGKISRDFVSAGNLIRMNDTVLTKVVSVDPIYFYFETSQNDLLKYIRLDRAGKR
                     LSSEKKPTPVLIKLPDEKDYLHQGTVDFLDNVIDSGTGTILARALVPNPDNVIYPGLF
                     ARVKLVGSGEYEAILLPDKAINTDQARKFVYVVDNENKVKRVYVELGPLRESGFYIIR
                     SGLKGDEKVIIHGIQRIHGPNQEVKPVVIEVTEN"
     gene            3339..6494
                     /locus_tag="I9760_001772"
     CDS             3339..6494
                     /locus_tag="I9760_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAT5043540.1"
                     /translation="MKIALFFIDRPVFATVISIIIVMVGVLSYFNLPVEQYPQVVPPT
                     IQVYASYPGANAKTVAETVATPIEQEVNGVENMLYMDSQSTDDGQMRLTITFKLGTDL
                     DQAQVLVQNRVAVVEPKLPEEVRRLGITTNKNSPDLMLVVNMYSPNGQYDQTYIGNYA
                     ILHIRDKIRRIEGVGNVLVFGASEYAMRLWLNPDLMNTFNLTANDVLAAVRAQNVQVA
                     SGKLNLQPQKKQYGFEYHIETKGRLVKKEEFENIIVKSGESGRIVRLKDIGRVELGTQ
                     NYLTKGYLDKYPAVALPIYQRPGTNALATAQEIIKTMKTIAKDFPPGITYDMAYNPTL
                     FVQQSIDAVFHTIYEAIALVVLVILIFLQTWRASAIPVVAIPVSLIGTFAVMQAIGFS
                     LNYLTLFGLVLAIGIVVDDAIVVVENMERNIQAGMNPRDAARKTMTEVGSALVAMGLV
                     LIAVFLPTVFLEGISGRFYQQFGTTLAVATAISVFVSLTLTPTMAVLLLRAHHGTIKQ
                     EGLAWWKKPIYGFLHGFNRLMEGFSRQYGKLVATLTRKTALMIIAYGIFISITLLLFV
                     YVPRGFIPRQDQGYFIVAVQLPPGASLSRTDAVINKAVNKILAIPGIAHTVSFTGFSG
                     ATFTNSSNAGAIFTPLLSFQERQRMGINYNDILKRLRQELSTIKEALIVVIPPPPVRG
                     IGNAGGFKMMLQDRGGRGLDVLMEAAATMVNAANQEKATTSVFTFFENSTPRLHLKLD
                     REKVERLNVPYANVVEALEVYLGSVFINEFNYLGRTFRVIAQADSEYRHTEDDILRIK
                     VKSNSGDMVPIGSVAQIENTVAPSRMPRFNLYPAIDLQGDVAPGYSSDEALVTMEKLA
                     QNNLPDGIGYEWTEIAYQQKMVGNTALMAFTLGVIFIFLVLAAQYESWSLPLAVILIV
                     PMCLFSSMLGVKILGMENNIMTQIGFLVLIGLASKNAILIVEFARQLENRGYNLWKAA
                     IQAAKLRLRPILMTSFAFILGVFPLIISTGAGAEMRRALGVAVFSGMLGVTFFGLVFT
                     PLFYVLISRLSRYKRKLKDLN"
     gene            6652..10971
                     /locus_tag="I9760_001773"
     CDS             6652..10971
                     /locus_tag="I9760_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HAT5043541.1"
                     /translation="MIKSKTFSCYIIGEANITLQCADILLAGGHKLLGIISPSNTIKK
                     WCTANSIAYLENIKEFEKNHMDEEFDFLFSIVNSEIIPQKILRLPRYYAINYHNSPLP
                     KYAGLYATSWAILNGETQHGISWHIMNEVIDAGDILKQPTFPINDLDTAFSLNLKCYE
                     QGIYSFHELVEELSSHTTTSVKQNLSCRSYYGLKNKPANFGFISWEESSESIDRLCRA
                     LTFGNYTNQLEVPKIMINKEIYVVKSYEKLNISSGVKPGTVVNNSNGGLQVATGTTDI
                     LILELTDLEGRTCSMEELGSLFKMTRYSQLDTIEPAFFEKLTICPAKKPKIEKFWVNE
                     FLKCAQEKNSFLSPLSQLDKTSCSLHDRKTVTQVPGELLAKLKKLCPEHWQIKNILLT
                     SLLIYLYRLNNYSNLSIAFINSQLSSNDPDLNKLLSDYVPLTTQFDSTMTFMDALTFV
                     SKEQGKLYENSTYTRDIFIRYPELHNAFNHIDVSITYITDSKAVAPCVGDKKLNFCIA
                     EDCSWFYVNNKTNYRAHLESYAFFENMNEHLLTLLEDIVSNPDKKIFELSIISKKEKN
                     DLLVVWNNTQCDYDYEKPIHQYFEEQVSKTPDEIAAVFENKSITYVELNRKANQLAHY
                     LRSQGVKQDDLVGISLSRSLEMVICILGILKSGGAYLPLDPNYPDERISYMLMDSKTN
                     LLITDQEFIKRKPHGFNGKSIEINSFLNLKNLSSENLQAINKPSDLAYIIYTSGTTGK
                     PKGVAISHRSICNHMLWMKKEYAFKNKDVFLQKTPFSFDASVWEFFMPLLVGGKLVVA
                     PNDAHTSPNQMIRLIRENKVSVLQIVPSMLKELVSNEEFGLCKSLTHVFCGGEALLSE
                     TINAFFKYNFSDTKLHNLYGPTEVTIDTTAYTCTAGDAKGDVSRIGKPIMNTKVYVLD
                     AKMQPVPIGIMGELYISGDGLARGYLNNPEFTIQKFLPNPFSNNKNDRLYRTGDLVKW
                     NSNGVLEYHGRCDNQVKIRGYRIEINEIESYLEKIPSIHQCIVKPEKNQDDSMSLSAY
                     LVLEKNSQISAVDIRAILKQNIPEYMIPARFYIVDKFFSTPSGKIDRKMLPIPSKRLR
                     SGTNYAPPNNPKEQLLHNIWCSVLKIDNLGIYDDFFELGGNSLSAMNIISHIREQFSL
                     TLSVRTLFDFPTISSLSKAIENIYHKKTDCIYNQSSGNHIIPLRTEGEKTPLFLVHPI
                     GGSVFWYKLLGQYFDKDRPLYGIQDPGLDRNELIFSNLEEMASAYVHSIQAIQPSGPY
                     ILGGASFGCTVAIEMAKQLEEKGETVISIISLDGWAFYPSLQNNELYFQEVMKEQNSR
                     ILKKYIENNVSNSKFLLELQWHRENMLTQYKMPIIKPKFILFKAKKLTEMFQYNASLN
                     WWENYTSQPIELHLTPGDHESMFYEPNIKILATKLNDSLNEQDIKHYQFNDTLKDSFI
                     DQL"
     gene            11092..13578
                     /locus_tag="I9760_001774"
     CDS             11092..13578
                     /locus_tag="I9760_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT5043542.1"
                     /translation="MPKKADLTLLLINTLEEPIFYLSSDFIILDLNASAKKIFKITKN
                     SIIGKPFSVLCSDHDLKSLEHPFDRNLKTYIRKKIVLWDSLCLKKPINGIQYIAIGTI
                     KNQTNKSALNSNLKTNTDGTNPIDITSKFLTEQINNKSKNTSDYVYAIYRYMENIIAQ
                     IPVSVYWMNKNHVYMGCSNNMVKLLNLNSRYDIVGKTYEDLYHEKSTSYYKKADKIVM
                     DKGISLSLEEPFYCPDGTTKEIYLSNKVPLRDLEGKIIGMLGVSVDITDRKKMELELS
                     KAKEAAEEANNVKTEFIANMSHDLRTPLSGVVGMAELMKEQVTDPVQVQYAEWIFDCS
                     QQLLSLLDGILDIVSLGNVKEDELKITTFDINECIAELVHLEMPSIKLKQLDLIVNID
                     KKIPRLIKTDRIKLHRIILNLLGNAIKFTDSGYIAIDVKLLELNNESGLIYFAISDTG
                     IGIPENQQTKIFDRFHRIIPSSIGKYKGHGVGLHIVQSYVNLLGGEIQVNSEPGVGTT
                     FYFELPIQIEKENEVIKGHYKLEKKNIIPVPKKEKILDKNSKNEFLYNSPNILILEDN
                     LVARRTIESLATQEKCRFVSVAKGKEAIRLAKTNEFDLIITDIGLPDIEGHVVAKLIR
                     EWELSQSKPPIPIIGLTAHVKEKSKEICLKSGMDDVFNKPISRQLLQYILSRFIPSKN
                     KNSTSCSSQKTTSSEQIIEADLPESESNFFNLDSYPLLDIHNAVSIMGSLKLVREILP
                     LMIGEETQKDVESIEKAFHIKDWETVERIAHKMKGGAANCGAVRMKYACQYLERYQKM
                     GYTKLLEPLYQQLIKVVEDTQKNIHEWLKH"
BASE COUNT         4559 a         2568 c         2567 g         4132 t
ORIGIN      
        1 ccaggatcta tttgatgaga agctgacaga aaaaattctg tacaggaacg cgcgtaattt
       61 ttacagtaaa aatagttcag agtaaggttt acactttata tttctctgtt tttctatttc
      121 taagtccttc atcttcttct acaggcacat ttgtaaatat cctcaaactc cctggactgg
      181 cagaagtata attaagacgg gctttttgat attgtttaca aatttctcgc tgaagctcaa
      241 tcggcatcat accaacagca ccatacactt tgacattacc gcttggtttc tttccaggag
      301 gataattttt aacatcaatc accgcctctc ttaaatcttt tataaattgc gtttcgaaac
      361 ctttgactaa tgtgtgcaca tgagtcaaac ataagtgaaa tccgtccgga ttttgtagca
      421 aattaagctt ccaacctcgc ttctccaatt catctgcaat gaaatgagca ttgcaagtgt
      481 tacttctaaa acccaggatg gaccattgag gattaccata aacataaata tcctcactgg
      541 ttaatccatt gccttcctcc agtaatgtcg ttaattcttt ttgaatcgca ttgcgtaatg
      601 tgataatgct tttagcgatt tcctgatatt tattttttcc atagtaagac aaagtggcat
      661 agacctctgc aacccgagcc ccactggttg aaccatcgag aattcctggt gtagcataca
      721 atccacctga ccagtttaat gcggcataaa ccgacaaagc aggcgaatcc tcactaaaaa
      781 gacaaacaga ggtgccttta ggacaacagc catatttatg caaatcggct gaaatggaag
      841 tcactccagg tactctaaaa tccataggtt ctgatgaagt atctaaaaaa gccgttaaaa
      901 atccacccaa acaggcatcc acatgaaaag gaacattttt tttctttgct aattgaccta
      961 actcagagac aggatcatga attccattca tgaaagatgg ggcagatcca acgattacag
     1021 cagtatttct ggtaatatag gaactcatga ccttgggatt caccgcaccc gtttttttat
     1081 caacaggaac cgtaattaat attgctccag ttaattcggc tgctttttta aatgcagcat
     1141 gtgctgtttc aggaacgaca atttcaggat aatcaatgcc ttttgccctt gcgcgaatga
     1201 cataagcagc catcgcctca ataatgctgg tcgtaccccc atgagttaat aaaccataac
     1261 attcttttga accatgaaac aaattttgac accagcgaat cacctccgct tgcattgcgt
     1321 taattctagg ccatttatca tgcaaaggat tagtgagagc agttgcccca taaacttctt
     1381 taagcagctc tgtcagttcc ttagggtgaa tagcgtataa tgcacctgaa tctttaccat
     1441 cccctctttc aacaagaaac tccctctcag ggctgtcatt agttacggat aaaaaatcaa
     1501 aatgacattt ttccacatcc acatcaaatg cactcaaaat atcttgggga gaaagccctt
     1561 cctccggtat ttcctccctc agagtcatac cggaacgctg aattctcaat ttttctctcg
     1621 ttgaagagag ttctttattg agctctttct caatgatctg attcacccca ggtaaatttt
     1681 ttgccaaggc gtaagcacta tcaataatac gctgcttcat actggcgtta ttacggctac
     1741 ggtaccacct ggaaatcgat gggttatggt attgattata aagaaaataa agagcagctg
     1801 tgcctaatat gatttgatgt gccggagtat cttgtaataa ttcatctaaa gaactgaccg
     1861 ctgctgttaa taaatctgaa acaaacccga acattgcaca tatccttacg aacaaaatga
     1921 tggataatag tcactttgca taaaaaaata aaggccggtc gatacgtgtc agtataacaa
     1981 aaacgccctg gtattttgct tgagccggat gatctcctaa tgcaagatat aatattcacg
     2041 gcattacgca taatgcgtct ttataagcta aactaagctt atccactgga tcataacata
     2101 ccagtgactt gaatatcagg gatagataac aatgaaaaaa aaactggcat tggtcatgcg
     2161 attactggct gttattctta ttcctgtcct catgtcctgt aattcagaat ccaataagcc
     2221 agtggtacat accttggcag aagttgacgt cgcgctacca gtaaaaaaga atattgtaga
     2281 atgggacgaa tacacaggac gatttcaagc agtagaagaa gtggatgttc gtgcccgtgt
     2341 cactggatat cttgaggcga tcaagttcca agacggccaa atggtaaaaa aaggagacgt
     2401 tttattcgtt attgatcaac gccccttcaa atatgctctg gcaagagcag aagctcaata
     2461 cgccttagcc aaagcgcaat atgaacgtgc cataaaactt caaaaagaga aatttatttc
     2521 agctgaagtg atagatcaac gatttcaaga cgtacaagtt gctgaaacca gagtacagga
     2581 agcaaaattg aatctcgaat ttactgaggt caaatccccc atcagtggca aaattagtcg
     2641 agattttgtg agtgcgggta atttaatccg catgaacgac acagtcttga ctaaagtagt
     2701 ttctgttgat cccatttatt tttattttga aaccagtcaa aatgatttgc tcaaatacat
     2761 acgcctcgat cgcgctggaa aaagactcag ctccgagaaa aaaccaactc cagtcttaat
     2821 taagttacct gatgaaaagg attacttgca tcaaggaaca gtggattttt tagataatgt
     2881 gattgactca ggcacaggaa ctatccttgc cagagcactt gtccctaatc ctgataacgt
     2941 catttatcct ggtctttttg cacgtgtaaa attagttgga agtggggaat atgaagcaat
     3001 tttactcccc gataaagcaa tcaatacgga tcaagcccgc aaatttgtgt atgtggttga
     3061 taatgaaaat aaagtgaaac gagtgtatgt tgagttaggg ccactgcgcg aaagcgggtt
     3121 ttacattatc cgaagtggat tgaaagggga tgaaaaagtt atcattcatg gaatccaacg
     3181 tattcatggc ccaaaccagg aagttaagcc tgtggtaatt gaggtgactg aaaattaaac
     3241 cggttttgta ttggaattat ggtcatttga tataatttta atactgcaca aagcatacaa
     3301 cgttttatgg tggagccctc acgaaaagga ttttctggat gaaaatagct cttttcttta
     3361 ttgatagacc agtatttgca acagtgatct ccatcatcat tgttatggtg ggggtattgt
     3421 cctatttcaa cttaccagta gaacaatacc cacaagtagt tcctcctacg attcaggtat
     3481 acgcctctta tcctggagcc aatgcaaaaa cagtagctga aactgttgca acaccgattg
     3541 aacaggaagt taatggtgtg gaaaacatgt tgtatatgga ttcccaatcc actgatgatg
     3601 gacaaatgcg tctgactatt acttttaagc ttggaacaga tctggatcag gcgcaggtgc
     3661 tggtgcaaaa cagggtggca gttgtggaac ccaaattacc tgaagaagtc cgccgcttag
     3721 gaatcactac taacaaaaat tcacctgatt tgatgttggt agtgaatatg tactccccta
     3781 atggtcaata tgatcaaacc tacatcggaa attacgctat cttgcatata cgcgataaaa
     3841 tcagacgaat tgaaggtgta ggcaatgtac tcgtatttgg ggccagtgaa tatgccatgc
     3901 gtctttggct gaatcccgat ttaatgaata cttttaattt aacggcaaat gatgttttag
     3961 cagctgttcg tgctcaaaac gtgcaagtcg ccagcggcaa gcttaatttg cagccacaga
     4021 aaaaacaata cggtttcgag taccatattg aaaccaaagg acgcctggtt aaaaaagaag
     4081 aatttgagaa tattattgtc aaatcaggtg aaagcgggcg tatcgttcgc ctgaaagaca
     4141 ttggtcgcgt cgagttaggt acgcaaaatt acctgacgaa aggttattta gataaatacc
     4201 ctgctgttgc attacctatt tatcaacgcc caggaaccaa tgcattagcc actgcacaag
     4261 aaatcattaa aaccatgaaa acaattgcca aggatttccc gccgggcatt acctatgaca
     4321 tggcctataa tcctactcta tttgttcaac agtcgatcga tgcggtattt cataccattt
     4381 atgaagcgat tgctctggtt gtactcgtca ttttgatttt tctacaaaca tggagagcct
     4441 ctgccattcc agtagtagct attcctgtat cattgatagg aacttttgcc gtgatgcagg
     4501 ctattggatt ttcgttgaat tacttgacct tgtttggact tgttcttgct attggtattg
     4561 tggttgatga tgccattgtc gttgtcgaaa atatggagcg taatatccag gcaggcatga
     4621 atccccgcga tgccgccaga aaaaccatga cagaggtagg atcagcattg gttgctatgg
     4681 gtttggtgtt gattgccgtc tttttaccta ccgtttttct ggaaggaatt tccgggcgtt
     4741 tttatcaaca attcggtaca acgcttgctg tcgcaactgc catttctgtt tttgtctcgt
     4801 taaccctgac tccaaccatg gcggtattat tgcttcgtgc tcatcacgga actataaaac
     4861 aagaagggtt agcctggtgg aaaaaaccaa tatatggatt tttgcatgga tttaaccgac
     4921 taatggaagg attttcccgg caatatggga aattggttgc taccctcaca cgtaaaacgg
     4981 ctttaatgat tattgcttat ggtattttta tttccatcac actacttcta tttgtctacg
     5041 taccacgcgg ctttatcccg agacaagacc aaggttattt tatcgttgca gttcaattgc
     5101 cgcctggagc ctctttgagc cgtaccgatg ctgtcatcaa caaagcagtc aacaaaatat
     5161 tggcgattcc tggtattgcc cataccgtta gttttaccgg cttttctggc gctacattta
     5221 ctaattcatc gaatgccgga gcaattttta cccccctatt gtcatttcaa gaaaggcaac
     5281 gtatggggat aaattataac gatattctta agcgtctaag gcaggaatta agtaccatca
     5341 aggaagccct catcgtggtt atccctcctc ccccagttcg aggtattgga aatgcaggtg
     5401 gttttaaaat gatgcttcaa gacaggggtg gtcgaggtct tgatgtatta atggaagcgg
     5461 cagcaacaat ggttaatgca gcgaatcagg aaaaagccac cacatcagta tttacttttt
     5521 ttgaaaattc aacaccacga cttcacttga aacttgacag agaaaaggtg gaacggctta
     5581 acgtgcctta cgccaatgtc gtggaagcac tggaagttta ccttggctct gtttttatta
     5641 acgaattcaa ttacctggga cgtacttttc gtgttattgc gcaagctgat tcagaatatc
     5701 ggcatactga agatgatatt ttacgaatca aagtcaaaag caacagtgga gacatggttc
     5761 ctataggatc agttgcgcaa atagaaaata cggttgctcc ctcgcgtatg cctcgtttca
     5821 atctgtatcc cgctattgat ttgcagggtg atgttgctcc cggatacagt tctgatgaag
     5881 cactggtgac tatggaaaaa ctggctcaga acaatcttcc agacgggatt ggttatgagt
     5941 ggacagaaat agcctatcaa cagaaaatgg taggtaatac tgctttgatg gcttttactc
     6001 tgggtgttat tttcattttc cttgtccttg ccgctcaata tgaaagctgg tctttacctt
     6061 tagccgtcat attaattgta ccgatgtgtc tcttttcttc catgctggga gttaaaattc
     6121 tcgggatgga aaataatatt atgactcaaa ttggttttct ggttttgatt ggacttgcat
     6181 cgaaaaatgc catcctcatt gtggaatttg cgagacaact tgaaaatcgg ggctataacc
     6241 tctggaaagc cgccattcag gcagcaaaac ttcgtctgcg cccgattttg atgacctcat
     6301 ttgcatttat tctcggtgtt tttcccttga ttatctcaac aggcgcaggc gctgaaatgc
     6361 gccgagcgct cggtgtcgct gtattcagcg ggatgttggg agtgactttt tttggtctgg
     6421 tctttacgcc tttattttat gttttaatta gtcgcttgag tagatataaa aggaaattaa
     6481 aagaccttaa ctaaatattt gctgtgaaac ccttaatata tttatgcact cggttcctct
     6541 cttctttgca tgatgtcgtg agtgaaattt taatcaacct tgactaaaaa agatcatttc
     6601 aattaaaatg tggcttttat taatcgatcc agtctgacag catggagcac tatgattaaa
     6661 agcaagacat ttagttgcta tattatcggt gaagctaata taactcttca atgtgcagat
     6721 atcctattag caggaggcca taaattactt ggcataatct ctccatcaaa caccataaaa
     6781 aaatggtgta ctgccaattc cattgcttat cttgagaaca tcaaggaatt tgaaaaaaat
     6841 catatggacg aagaatttga tttcttgttt agcatagtca acagtgaaat cattcctcaa
     6901 aaaattttaa gactccctcg ttattacgcc ataaactatc ataactcccc attacccaaa
     6961 tatgcaggat tatatgccac ttcgtgggcg atccttaatg gcgaaactca gcatggtatt
     7021 agttggcaca ttatgaatga agtgatagat gcaggtgata ttctaaagca accaactttt
     7081 ccaataaatg atctggatac ggcgtttagt ttaaatctga aatgttatga acaagggatc
     7141 tattcctttc atgaacttgt tgaagagctt tcatcccaca ctacgacttc ggttaaacaa
     7201 aatctgtcat gccgttctta ttatggtcta aaaaacaagc cggcaaattt tggatttatt
     7261 tcatgggaag aatcttcaga aagcattgat cggctctgca gggctttaac atttggaaat
     7321 tataccaatc agttagaagt acccaagatt atgattaata aggaaattta tgttgtaaaa
     7381 tcttatgaaa aattaaatat atcctcagga gtaaaaccag gcaccgttgt gaataactcg
     7441 aatggtggct tacaagtagc taccggaaca acagatatat tgattctaga gcttactgat
     7501 cttgagggca gaacatgctc aatggaagaa ttaggcagct tatttaaaat gactcgctac
     7561 tcacaacttg acacaattga acctgcgttt tttgaaaagc taacaatttg tcctgctaaa
     7621 aaaccaaaaa tagagaagtt ctgggtcaat gagtttttga aatgtgctca agaaaagaat
     7681 tcatttttat ctccactatc ccaactcgat aaaacatcat gttcacttca cgatagaaaa
     7741 acagtaacac aagtacctgg tgagttactc gcaaaattga aaaaactatg ccctgagcat
     7801 tggcaaataa agaatatctt attaactagc ctcttaattt acctatatcg attaaataat
     7861 tacagtaatc tctcgattgc attcattaat tcacaattga gcagtaatga tcctgattta
     7921 aataagcttt tatcagacta tgtgccttta acaacgcaat ttgatagcac tatgacattt
     7981 atggacgcat tgacatttgt ctcaaaagaa cagggaaagt tatatgaaaa cagcacttac
     8041 actagagata ttttcatacg ctaccctgag ctacataatg ctttcaacca tattgatgtc
     8101 agtatcacct atattactga ctcaaaagca gtggcacctt gtgtcggcga taaaaagtta
     8161 aacttctgta ttgcagagga ttgttcctgg ttttatgtta ataataaaac aaattataga
     8221 gcccatttgg aatcttatgc tttttttgaa aatatgaatg agcatttgct gactttattg
     8281 gaagatatag tcagtaaccc tgataaaaag atattcgaac tttcaataat cagtaaaaag
     8341 gaaaaaaacg atttattggt agtctggaat aacactcagt gtgattacga ttatgaaaaa
     8401 ccgattcatc aatattttga agagcaagtt tccaaaacac ctgacgaaat agctgctgtc
     8461 tttgagaaca aatcaataac ttatgtagaa cttaaccgaa aagccaatca attagcacac
     8521 tatttaagat ctcaaggtgt aaaacaagat gacctggtag ggatttcttt aagtcgcagt
     8581 ttggaaatgg ttatatgcat tttaggcatt ttaaagtctg gaggagctta cctgccatta
     8641 gatcctaatt atcctgatga gcggatttca tacatgctaa tggacagtaa aactaacctg
     8701 ctgataacag atcaagaatt cataaagaga aaaccacatg gattcaatgg caaaagtatt
     8761 gagataaatt cctttttaaa tttaaaaaac ctgtcttctg aaaatcttca agcaattaac
     8821 aaaccatccg atttagccta cattatttat acttccggga ctacaggaaa acctaaaggt
     8881 gtagccatat cgcatcgttc tatttgcaat cacatgttgt ggatgaagaa ggaatacgct
     8941 tttaaaaaca aggatgtatt tttacagaaa acccccttct cgtttgatgc ctctgtttgg
     9001 gagtttttta tgccacttct ggttggtggt aaactggtcg ttgccccaaa tgatgcacat
     9061 accagtccca atcaaatgat acgcctaatc agagagaaca aagttagcgt attgcagatc
     9121 gttccttcta tgctaaaaga attagtatct aacgaagagt ttggtctctg caaatcactc
     9181 acgcatgtat tttgtggtgg tgaagccctt ttatcagaaa caattaatgc tttttttaag
     9241 tacaacttct cagatacaaa actacataat ctctatggac caacggaagt aaccattgat
     9301 acaactgctt atacatgtac cgcaggagat gcaaaaggcg atgtaagccg aataggcaaa
     9361 ccgataatga acacaaaagt gtatgtatta gatgcaaaaa tgcagcctgt tcctattgga
     9421 ataatggggg agctttatat ttcaggagat ggtttagcac gtggttattt aaacaaccct
     9481 gaatttacca tacagaaatt tttacccaac ccattcagca acaataaaaa tgatcgatta
     9541 tacagaacag gggatcttgt caaatggaat tccaacggag tcctggaata tcacggccga
     9601 tgtgataatc aagtaaaaat tagaggatat aggatagaaa tcaatgaaat tgaatcctat
     9661 ttggaaaaaa ttccttccat ccatcaatgc attgtgaaac cggaaaaaaa ccaggacgac
     9721 tcaatgtctt tatcagctta tttggttttg gaaaaaaatt ctcaaatatc cgctgtagat
     9781 attcgggcta tattaaagca gaatattcct gaatacatga tcccggcccg attttatatt
     9841 gttgacaaat tttttagcac cccaagcggt aaaatagata gaaaaatgtt accaatccca
     9901 tccaaacgat tacgttcggg tactaattat gctcccccca ataatcctaa agaacaatta
     9961 ttacacaata tctggtgttc ggttttaaaa atagataatt tgggaattta cgatgatttt
    10021 ttcgaacttg gcggtaattc actatcagca atgaacataa tatcacatat acgggaacaa
    10081 ttttcactga cattgagcgt tagaacatta tttgattttc ccacaatatc ctctttatct
    10141 aaagcaatag agaacatata ccataagaag actgattgta tatacaatca gtcttctgga
    10201 aatcatatca tccctcttag aacagaagga gaaaaaacac ctctgttttt agtacatcca
    10261 attggtggaa gtgttttttg gtacaaactt cttggacaat attttgataa agatagacca
    10321 ttgtatggaa tacaggatcc tgggcttgat agaaatgaat tgatatttag taacctggaa
    10381 gaaatggcta gtgcatatgt ccattcaatt caggctattc aaccaagcgg accttacata
    10441 cttggtggcg catcatttgg atgtactgtt gctattgaaa tggcgaaaca attagaggaa
    10501 aaaggagaaa cagtaatttc tattatctcc cttgatggtt gggcatttta cccttcctta
    10561 caaaacaatg agctttattt tcaagaagtt atgaaagaac aaaattcaag aattctaaag
    10621 aaatatattg aaaataacgt cagtaactcc aaatttttat tagaattaca atggcataga
    10681 gaaaacatgt tgactcaata taaaatgcca attattaaac ccaaatttat tttatttaag
    10741 gcaaaaaaac ttacagaaat gtttcaatat aatgctagtt tgaattggtg ggaaaattat
    10801 acaagtcaac caattgaact acatttaact cctggtgatc atgaaagcat gttttatgag
    10861 cctaatatca aaatcctggc taccaagctc aatgatagct taaatgagca ggatattaag
    10921 cattaccaat tcaatgatac actgaaggat tcatttatag atcaactcta aaccagtatc
    10981 atcagaactg cacagaggtt ttggttgatt tgtttcttct ctctccgtga cctattattt
    11041 aaatggaata aataaaatcc atatgaatga aattgaatta ggtcactctc tatgcctaaa
    11101 aaagcggatc tgacattgct cctcatcaat acccttgaag aaccaatatt ttatctttcc
    11161 tcagatttta ttattttaga tctcaacgct tccgccaaga aaatattcaa aattacaaag
    11221 aattcaatta ttggaaaacc tttttcagtt ttatgttctg atcatgattt aaaatcactt
    11281 gaacatcctt ttgatagaaa tttaaagaca tatattagaa aaaaaatagt tttatgggat
    11341 tcattatgtt taaaaaagcc tataaatgga attcaatata ttgctatagg gactattaaa
    11401 aatcaaacca ataaatccgc attgaatagc aatctcaaaa caaacacaga tggcaccaac
    11461 cctatagaca tcacaagtaa atttttaacg gaacaaataa ataataaaag taaaaatact
    11521 tcagattatg tatatgctat ttatcgctac atggaaaata ttatcgcaca gattccagta
    11581 agtgtttatt ggatgaataa aaatcatgtg tacatggggt gtagcaataa tatggttaaa
    11641 ttattaaatc tcaattcaag gtacgatatt gttggtaaaa cttacgagga tctatatcac
    11701 gaaaaatcaa cctcatacta taaaaaagca gataaaatag ttatggataa aggtatctca
    11761 ttaagtctgg aagagccatt ctattgtccc gatggaacta ctaaagagat ttatttgtcg
    11821 aataaagtcc ctcttcgtga cctggagggt aaaattatag gtatgctcgg ggtatcagta
    11881 gatattactg atagaaaaaa aatggagttg gaacttagta aagctaaaga agctgctgag
    11941 gaagcaaaca atgtaaagac tgaattcata gccaatatga gccatgatct cagaacccca
    12001 ctaagtggtg ttgtgggtat ggctgaactt atgaaagagc aagtaacaga tcctgtacaa
    12061 gtacaatatg cggagtggat ttttgattgc agccaacaat tattgagttt attagatggc
    12121 atacttgaca tcgtatcctt gggaaatgtt aaagaagatg aattaaaaat aacaaccttt
    12181 gacatcaacg aatgtattgc ggaattagtt catcttgaaa tgccgtccat taaactgaaa
    12241 caacttgatt tgatcgttaa tattgataaa aagattcctc gattaataaa aaccgaccgt
    12301 ataaaacttc atcgaattat tctcaatctt ttaggtaatg ccattaaatt taccgattcc
    12361 ggttatattg caattgatgt gaaattactt gagctaaata atgagtcagg attaatttat
    12421 tttgcaatat ctgatactgg cattggtatc cccgaaaatc aacaaacgaa aatatttgac
    12481 agatttcatc gtattatccc gtccagcata ggaaaatata aaggccatgg cgttggttta
    12541 catattgttc aatcctatgt aaacctgcta ggcggagaaa tacaggtaaa cagtgaaccg
    12601 ggagtaggaa ctacctttta ttttgaactc cctattcaaa ttgaaaaaga aaatgaagtg
    12661 ataaagggtc attataagct tgaaaaaaaa aatataatac ctgtacctaa aaaagaaaaa
    12721 attttagata aaaactcaaa aaatgagttt ttgtataact cacctaatat tctcatcctg
    12781 gaagataatc tggtagccag acgtacaata gaaagcttgg cgactcaaga aaaatgtcgt
    12841 tttgtttctg tagcaaaagg taaagaagcc atacggcttg ctaaaaccaa tgaatttgat
    12901 ctgataatca cagatattgg gttaccagat attgaaggac atgtggtagc aaaattaatt
    12961 agagaatggg agttatctca atcaaagcca ccgataccaa taataggatt aactgcacat
    13021 gtcaaagaaa aatccaaaga aatatgcttg aaatcaggta tggatgatgt attcaacaaa
    13081 ccaatcagtc ggcagctttt acagtatata ctgagtcgat tcattccatc aaaaaataaa
    13141 aactcaactt catgctcttc tcaaaagaca acatcatcag agcaaataat agaagcagat
    13201 ttgccagaaa gtgaaagtaa tttttttaac ttggactctt atcctttact cgatattcac
    13261 aatgctgttt ccatcatggg tagtctcaaa ttggttcggg agatattacc acttatgata
    13321 ggtgaagaaa cccaaaaaga tgttgaatcc attgaaaaag cttttcatat aaaagattgg
    13381 gaaacggtag agagaatcgc tcataaaatg aagggtggtg ctgcgaattg cggtgctgta
    13441 cgtatgaaat atgcttgcca atatttagag cgctatcaaa agatgggata taccaagctt
    13501 cttgagcctc tttaccaaca acttataaaa gtcgtggaag atacccaaaa gaatattcat
    13561 gaatggctaa aacattgacc ctcactagaa acatcaatgg tattcactaa ttaagccccc
    13621 agaaaaaact tcaaatataa tatttatcaa tcaacttgaa tattataaat acacattaat
    13681 taactttgct ataattaatt taaataacgc caaattggat tcggtgtaac ctttgggagg
    13741 aagcagtaaa tgtctgatga aataagtaaa gaatattttg atgagactct cggcatccat
    13801 caagaatacc tgggtatcct taattg
//
LOCUS       DACTJE010000080        13730 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.080, whole genome shotgun sequence.
ACCESSION   DACTJE010000080 DACTJE010000000
VERSION     DACTJE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13730
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.080"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            151..5916
                     /locus_tag="I9760_001775"
     CDS             151..5916
                     /locus_tag="I9760_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HAT5043543.1"
                     /translation="MNKNPFSLIMNVLCSIFSTLFGKIKWNRPTWLVYLKHKSKNSPQ
                     IFWGTTLLFIVLLGAGAYGAYWYNNLPKPTYTTASITIPDITPNEENLVPNNLIIDFG
                     IRNNEFIPQSVAPINLIGKEIDEGIEMAPQIAGKWSWNSDSQLIFIPAEDWPAGQKYT
                     IHFAKNFFAPNANIESYDLSFTTKPFQATIREFKFYQDPVNAETRQAVATIEFNYPVN
                     PKNLEKNTSVVFQTIKNGKADLTAESVPFSYTYDNNKRIAYLHSANIKIEDVSRYLLL
                     TLNKNVSSSTDSAILGQSISKNLLIPDASNFLKIVSTSANIVRNDKDRPEQVLSIETS
                     LGVNESEFNKSVHVYLLPQNYPATAAEPEKSNYEWQNPGEVTNNILSLSTPLPKEVIP
                     SEQNYTTLHGFKFKAQTPRYLYIKIDKGMKGFGGFKLSNDYTAIVPVPEYPKEISFLH
                     KGSLLALSGEKKLSVLIRGVPAVKYEFARVLPDNVNQLVTQTQGDFSNPYFINPSFNQ
                     QNISQIFSEIQQYDSTDLTRQQYTSLDLGKYLASEANASGPQGLFLLQATGWDVANQY
                     ALDVKASRLILITDLGMVVKDNNDGSHDVFISSITQGLPVAGATVTILGKNGLPILSR
                     VTDEQGRANFPTLKDFIEDREPTVYLANLNNDVSFIPFNNYNRQLNFSKFDIGGIYTN
                     DQDLQNLTAYLFSDRGIYRPGDTVHVGMIVKRAFAEPQPSGLPLQATVVDARGTTIKD
                     QKVTLNDTGYIELDFTTSANSPTGQYMVYLYLVKDNHPQNLLGSTSVRISEFQPDRMR
                     ITSSFEPKPFDGWSSPTDLKAKVDLWNLYGAPAANRRVSGKILLTPQRVQFDNYPDYI
                     FADPLYDPKKPPKVFTENLAEATTNENGQAEFSLNLERFEKATYQLTFFAEGFEPGSG
                     RSVTAQTKTLVSPLPYFIGYKADGDLHFIKQNSVRNVNYIAINPQLGKEDVKNLKIQL
                     ISLRPVTTLVKKADGTYQYQSTIQSTVINTNPFEIKESGINYSLPTNTIGDYSLNVLD
                     KDDSILSQLQFSVVGTSQAPLAKNAELSIKLNKEEYQANEDIEIQITAPYTGSGLITI
                     ERDKVYAVHWFKTEAQNSVQTIRIPADFQGNGYVNVAFIRDWESPELFISPLSYSVAP
                     FNVNHDKHDIKIGLKTPELARPGESFTIDYHTDKPGKIIVFAVDEGILQVANYTTPDP
                     LSFFFQKRALEVLTQQTVDQILPKFIQDREISAVGGDEGEAALASRLNPFKRKTDLPV
                     VYWSGILDSDSTDRQLSYQIPDYFNGSIRVIAVAVANDSVGSREVSAEVRGDFIISPN
                     VPTFVAPGDEFEISTTVANNIKDSGEHAQVEVELTASPEIEVIGSAKESLEISEGNEQ
                     TVYFKLRAKSLLGSARITITAKSGDKSSKMDSTLSVRPANPFTTYINSGKSQDSQKSL
                     EVIQTLYPEHRKVEATISTSPLVLIFGLQRYLDNFPYGCTEQLTSKALPWLVMNSLPW
                     FSSESGPIHEKINTTIQMLSQRQMSNGGFSYWPGLSDNSANSFASVYAMHFLTEARAQ
                     GFNVPGEMFYNGINYLKELASHNPTDADSARIQAYAIYILTRNELVTTNYLANLQLYL
                     QQDSSKNWLKDITAVYIAATYQLLKSYSEANQLIGQYQPATNNAFTTDFYDRNIADAQ
                     YLYLVAKHFPNLLPQVSDNILMNLVKAINNDEINTVLSGFSSLALGAYPQNESAEQSD
                     KLSITEALANNQQRNIPADQNNYQKLNIDETVKTIRFNNPDKQTFFYQLVQSGFDKII
                     PTEPLKQGIEIYREYRDEKGNVIDSAVLGEEIEVHLQVRSLDNNYLSNIAIEDLLPGG
                     FEVVRDSVKTDNMDYADVREDRVNFFGWVDLTAKELVYKIKATNVGKYIVPPAYAEAM
                     YNPNTKARGSSSVITVSREED"
     gene            complement(6181..7584)
                     /locus_tag="I9760_001776"
     CDS             complement(6181..7584)
                     /locus_tag="I9760_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1666 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043544.1"
                     /translation="MLKFVIYLSSRVQPMGKKAYIIGMGPAGLASALALLVKGYSVEL
                     IDRRSEDDVFSRKQGVFLKDDTIRDFFALSQLASKGYSLEFKDNFICKLIRPIDESNL
                     QLSAEDQLDLAFFELIEKERTVIPIAELQRYQYSKLKYIYDKGGFTFQDEEDPYTVKF
                     NNAEQQLIFHLGDTQIAKVDAENQKLTLDHNGSIQNHSFDLLVDASGKTSKSFTQLWN
                     SQNPEFAIGYEKLDNPDHNAFGVMYLSINNPPLAMIANPVLSPTEGTSFIPLDKITPL
                     KAMGWTHDYPPLIYLKYSKKNGAVYLTGEIPESILKDNNKKELKQWFDLVLQLLFNNT
                     DFKMSAPGLASVFKTDIEIADKFALLLPKGGVFCLVGDALMSANFLLGCGISNALDDA
                     NKLPDMVDNQFSQSEAEAYRNLRYLDYKDDWLFFKEHIALRLKSLQLEKQLDLSRQEL
                     ELKQRELESIQQQCSFY"
     gene            complement(7746..9137)
                     /locus_tag="I9760_001777"
     CDS             complement(7746..9137)
                     /locus_tag="I9760_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1667 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043545.1"
                     /translation="MEYKEVTPMIRFFIICFIFLVSHLAYANPYWDRYKVPRDSHYPN
                     LKFHYINPDSLKIVQENSGIDVLRIQTDWFEETDSYQVGTVIIESSGTPALLSRSIHK
                     PKWGSYLGVLKSRFGGDTVYYDAIGTGKEYRKLARAINLRFPVPSHDMIFELYAENPQ
                     TGLMEKVVEKNIIASELTRDNRHFNDLEVRQLLPAVKTPSLRVNIYAEGYTQSEKMKF
                     WNRAIKTVQALQSQQFPGIDYMDFYAVFNPSHKRLGVAEDLGLPVPEFDSFLGLYYPY
                     WDNFGRWYHIVYPTRENKFRQGLASAPYDYPIVLVNNSQYWGVGNYKSHTAIPADNST
                     YFTYILIHELGHFFGLNEEYEGGGRTELEFAPEMKEPWSQNITFLTTPSYSGLKWNEL
                     VNPQMSIPTPYSDWQSTPPQYGAYRGGYGDSPSHQGASHKPGLNCIMESHASFCDICQ
                     KGIMDVIRFDLGT"
     gene            complement(9285..10109)
                     /locus_tag="I9760_001778"
     CDS             complement(9285..10109)
                     /locus_tag="I9760_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043546.1"
                     /translation="MKKIAIILSLFPFLICCGLANATASQKRLPGESYQDAVKRIINN
                     KPQNELKMLIARGTPYSKKVSLQKIDYSTVPKVSSYDEMTNMFNLIRDTRFLYSPDKP
                     DFQRRISWLYPDDGCFARAALSRIKLDSEHFVIPAKIFVFGDLEMQTPYSSEGTVSWW
                     YHVSAVVNYMGTIYVLDPAAKPEGPMLIDDWYNKIGIMDKMEAVVCNPYTYDPFDRCY
                     DATNKTDNKALNDQLKYLDKEWVRIESLGFDPSVLLGDNPPWIGDMRNSTLLSYKS"
     gene            10372..10898
                     /locus_tag="I9760_001779"
                     /pseudo
     CDS             10372..10898
                     /locus_tag="I9760_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11075..13300)
                     /locus_tag="I9760_001780"
     CDS             complement(11075..13300)
                     /locus_tag="I9760_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAT5043547.1"
                     /translation="MAESKLEIVNKTEREESVILDGNGILRCYNMFPTQFGSIEEMID
                     YLPQLQAMGFNAVWINPVQTAGDVQGLFKRDKTIGVRAYNEVTRSLYAMTDCDLISPY
                     FNPLPPNVDPKLKKQLDMEAMQKFTSEARNNGLVPMFDLVLNHVAADSRHRKEHPEWF
                     QKDVHPDFKDAIAFDYSDPQIRDEIIEQFWKPYIYKYMIDYGFEGVRIDAVGYLNPEL
                     RKQIYAYIYDLAEQFNKPKPVILDELLFSGSQKLTDVVDGLLLPEKGPTHITRGTYYA
                     QRDGYGGLPGWCKEEEGVKAQVVFLDKQKKLHPHAKGGCIAFSGNHDHNSLAMTILEE
                     MAEHRLANHHTLHGAQEKFKHERSYDYDEALESVLRYSFVKDIQNEILSGNESTIKEV
                     ETRMREKIAFCALTSSGGWYALSGDETGDLLAKPVFRRVHALDQTYYAQRTHIIFDKK
                     YPDNKVAMEVLTEMAIENIQKEKAGSIYNSLQHDKQSQERLLVPYLETLQNQINCGDT
                     VVCKAFQEKLAQKNITIGFTDENYSPDPRTPQNGWNGQHDMRKFMFEINSILSSLPSS
                     NMGFWSEVIPIQSKPGLLAVVRKNGLGLSSDTDIAIVNMDPSQKVELTRDDIHQLACN
                     FQKRVIPEYTYYNDGKDKQYNWHTNNPDFNMAYHTVMSCIEAKRIHVDDSIKTNFPVH
                     SANPYGMFSKKQSSKMNIAKIAKMSGEDTTLIEKGTNDTEGIIHDDEQIQPTITVENK
                     K"
BASE COUNT         4412 a         2702 c         2413 g         4203 t
ORIGIN      
        1 taaattatat tcaaattaat attgaaatgc attagaattg gcaaaggata gactaataat
       61 taataattta ttgcgggggg tgtatgcttt accgcaccct cattaccaga tcctaaaata
      121 aacttagacc aaccaggact ccatatcacc atgaacaaaa acccattctc cctaattatg
      181 aatgtattgt gttcaatatt ttcaactcta ttcggtaaaa taaaatggaa tcgcccgacc
      241 tggttagttt atttaaaaca caaatcaaaa aactctcccc aaatattttg gggaacaact
      301 ctattattta tcgtgttatt gggtgcagga gcatatggtg cttattggta taataatttg
      361 ccaaaaccaa cttatactac tgcatccatc acaataccgg acatcacacc taatgaggaa
      421 aaccttgttc ccaataatct cattattgat ttcggcatta gaaataatga atttatccca
      481 caatcagttg ctcccattaa tttaattggc aaagaaattg atgaagggat agaaatggca
      541 ccgcaaattg cgggcaaatg gagctggaat agtgatagcc aattaatttt tattcccgct
      601 gaagattggc ctgcagggca aaaatatact attcattttg caaaaaattt ttttgcaccc
      661 aatgcgaata ttgaaagtta tgatttatcc tttacgacaa agccctttca ggccacaatc
      721 cgcgagttca aattttacca agatcccgtc aatgcagaaa caaggcaggc tgtagccact
      781 atagagttca actatccagt aaatccaaaa aatcttgaaa aaaatacttc cgttgtattc
      841 caaaccatta aaaatggaaa agcagacctc actgcagaat ccgttccttt ttcttatact
      901 tatgataata ataaacgaat tgcctatctt cattcggcaa atatcaaaat agaagatgta
      961 tcgcgttatt tacttttaac tttgaataaa aacgttagtt cttcaactga ttcagcaatt
     1021 cttgggcagt caattagtaa aaatttgcta atacccgatg caagtaactt tctaaaaata
     1081 gtttcaacat cggctaatat agtccgtaat gataaagaca ggcccgaaca ggtacttagt
     1141 attgagacca gtttgggtgt taatgaaagt gaatttaata aatcagtaca tgtctatttg
     1201 ctcccgcaga attatcctgc taccgctgca gaaccagaaa aatccaatta cgaatggcaa
     1261 aatccaggtg aagtaacgaa taacattttg tctctgtcga ctccattacc caaagaggtg
     1321 atcccttcgg aacaaaatta tactacgttg catggcttca aatttaaagc tcaaactccc
     1381 agatacctgt atatcaagat tgataaagga atgaagggtt ttggtggttt taaattaagc
     1441 aatgattaca cggctatcgt tccagtacca gaatacccca aagaaatcag tttcttgcat
     1501 aagggctctt tgttagcact gagtggtgag aaaaaactgt cagttctgat acgcggagtg
     1561 cctgcagtta agtatgaatt cgcgcgtgtg ttgcctgata atgtcaatca gctggttacg
     1621 caaacccaag gcgatttcag caatccttat tttattaatc cctcttttaa tcaacaaaac
     1681 atcagtcaaa ttttctcgga aatccagcag tatgactcca ctgatttaac cagacagcaa
     1741 tacacatccc tggatctggg aaaatattta gcatctgaag ccaatgcctc aggaccgcaa
     1801 ggattgttct tattacaagc aacaggttgg gatgtagcca atcagtatgc tctcgatgtt
     1861 aaggccagtc gtttgatatt gattacggat ctggggatgg tggtaaaaga caataatgat
     1921 gggagccatg atgtctttat tagctccatt acacaaggac tcccagttgc aggggcaaca
     1981 gttacgattt taggtaaaaa tggcttgccc attttatctc gtgttacaga tgaacaaggg
     2041 cgagcaaatt ttcctactct gaaagatttt attgaagaca gagaaccaac tgtgtatttg
     2101 gcaaacctga ataatgatgt gtcttttatt ccattcaata actacaacag gcaattgaat
     2161 ttttcaaaat ttgatattgg aggaatctat accaatgatc aggatttgca gaacttaaca
     2221 gcttatttat tttctgatag aggaatatat aggcccggag acacagtgca tgttggaatg
     2281 attgtaaaac gagcctttgc agaaccacaa ccttctggtc tgccattaca agccactgta
     2341 gtagatgcca ggggaactac tatcaaagac caaaaagtaa cattaaatga caccggttat
     2401 atagagcttg attttactac cagtgccaat tctcccactg gtcagtatat ggtttattta
     2461 tacttggtaa aagataacca cccgcaaaat cttctcggtt caaccagtgt tcgtatttcc
     2521 gaattccaac ccgacagaat gcgtattact tccagctttg aacccaaacc ttttgatggc
     2581 tggagctcac ccactgacct gaaagcaaag gtagatcttt ggaacttgta tggtgcccca
     2641 gctgccaata gaagagtaag cggcaaaatt ttattaactc ctcagcgagt gcaatttgac
     2701 aactatcctg attatatttt tgcggatcca ctgtatgatc cgaaaaaacc cccaaaagtt
     2761 tttacagaaa atctggcaga agcaacaact aacgagaatg ggcaggctga atttagcctg
     2821 aatcttgaac gttttgaaaa ggcaacttat caattaactt tttttgctga aggctttgag
     2881 cctggtagtg ggcgaagtgt tactgcacaa actaaaacat tggtcagccc tttaccctat
     2941 ttcattggct ataaagctga tggcgatttg cattttatta aacagaacag tgtcagaaat
     3001 gttaattata ttgcaattaa tccacagttg ggtaaagaag acgtaaaaaa tttaaagata
     3061 caattaattt ccctgcgccc cgtcacaact ctggttaaaa aagccgatgg cacttatcaa
     3121 tatcaatcca caatacaatc tacagtcatc aataccaatc cattcgaaat taaagaatct
     3181 ggcattaact acagcctgcc aacgaatact attggtgatt actccctcaa cgtattagat
     3241 aaggatgata gtattcttag ccaactgcaa tttagcgtag ttggtactag ccaggctcct
     3301 ctcgctaaaa atgcagaatt atctatcaaa cttaataaag aggaatatca agctaatgaa
     3361 gatatagaaa tccaaatcac tgctccttat actggctcag gcttgatcac tattgaaaga
     3421 gataaagtct atgctgtgca ttggtttaag acagaagccc aaaactctgt acaaaccatc
     3481 cgcatacctg ctgatttcca gggtaatgga tatgtgaatg tggcttttat tcgtgattgg
     3541 gagtctcctg agctcttcat tagcccatta agttatagtg ttgctccatt caatgtgaac
     3601 catgataaac acgatataaa aattggacta aaaacacctg aattagcccg tcccggagaa
     3661 tcttttacga ttgattatca cacagacaaa cccggtaaga tcatcgtatt tgcagtggat
     3721 gaaggaatat tgcaggttgc caattatact acacccgatc cactcagttt ctttttccag
     3781 aaacgagcac tagaagtgtt aacccaacaa acagttgatc agattttacc caagttcatc
     3841 caggatagag aaatatctgc tgttggaggt gatgagggtg aagcggcttt agcgagtcgc
     3901 ctcaatccat tcaagagaaa gactgattta ccagttgttt actggtcagg tattcttgac
     3961 tcagacagta ctgacaggca attatcttat caaatacctg attattttaa cgggtccatt
     4021 cgggtaatcg ctgtggcagt cgctaatgac tctgtgggat ctcgcgaagt ttcagcagag
     4081 gttcgcggtg attttattat aagtcccaat gtgcccactt tcgttgcgcc tggagatgaa
     4141 tttgaaatat caaccactgt ggcaaataat attaaagatt caggagaaca cgctcaagtt
     4201 gaagtagaac ttaccgcatc accagagatt gaagtaatcg gtagtgcgaa agagtctctg
     4261 gaaatcagcg aaggcaatga gcaaactgtg tattttaaat tgcgcgctaa atcactgtta
     4321 ggatcagcaa ggattactat cactgccaaa tcaggagata aatcaagcaa gatggatagt
     4381 acgctcagcg tcaggcctgc caatcctttc acaacataca ttaatagtgg caaatcacaa
     4441 gattctcaaa aatcgttaga ggttatccag acattatatc cggagcatcg caaggtagaa
     4501 gcgactatat ctaccagtcc attagttcta atctttggat tacaaagata tttggataat
     4561 ttcccatatg gttgtacgga gcaattgaca agcaaggccc tgccctggct ggttatgaac
     4621 agtttaccct ggtttagttc ggaaagtggc ccaattcacg aaaaaattaa taccacaatt
     4681 caaatgttaa gccaaagaca aatgtctaat ggtgggttta gctattggcc tggtctaagt
     4741 gacaattcag cgaatagttt tgcatcagtt tatgccatgc atttcttaac tgaagcaaga
     4801 gcacaaggat tcaacgtccc cggagagatg ttctacaatg gcattaatta ccttaaagaa
     4861 ttggctagtc ataatcccac agatgcagac tcagcaagaa ttcaagccta tgccatctat
     4921 attttaaccc gtaatgaatt agtcactaca aattatttag ccaatttaca attgtattta
     4981 cagcaagatt cttcaaaaaa ctggctaaaa gacatcacag cagtttacat tgcagccacc
     5041 tatcaattat taaaaagtta tagtgaagcg aatcaattga ttggccaata tcaaccagca
     5101 acaaataatg catttacgac tgatttttat gatcgcaata tcgccgatgc gcaatatctc
     5161 tatcttgttg caaaacattt tcctaattta ctgcctcaag taagcgacaa tatactcatg
     5221 aatttggtaa aagcgataaa taatgatgaa atcaatacag tcttatcagg attttctagc
     5281 ctggctttgg gcgcttaccc acaaaatgaa tcagcagagc aatctgataa gctgtccata
     5341 acagaggcat tggcaaacaa tcagcagaga aacatccctg ccgatcaaaa taattatcaa
     5401 aaattaaata ttgatgaaac tgtcaaaaca atccgcttta ataatcctga caaacaaaca
     5461 ttcttttatc agcttgttca gtcagggttt gataaaataa taccgacaga acctttaaag
     5521 caaggcatag aaatctacag agaatatcga gatgagaaag gcaatgtcat tgattcagcg
     5581 gttttgggcg aagaaattga agtacacctg caagtccgat cattagataa taattatctg
     5641 agcaacattg ccattgaaga tctcttgcca ggtgggtttg aggtcgttag agactcagta
     5701 aaaacggaca atatggatta tgccgatgtc cgggaagaca gagttaactt cttcggctgg
     5761 gtagatttaa ctgcaaaaga attagtttat aaaataaaag cgactaacgt tggtaaatac
     5821 atagttccac cagcctacgc agaagccatg tataacccaa atacgaaggc aagaggttct
     5881 tcatcagtca ttacagtgtc tagagaagaa gattaattgc tggcaagcaa agtcagattt
     5941 tgattcttga ctttgcttgc taataaatat taattgccct catatttgct catgccgaca
     6001 aatgaaggag atacttatca tagcttgagg atgagataaa actatttagg gaggaatcta
     6061 ttttgtagca aacactgttg gatcttaaag aatagattcc tcagcccggc atggtaatgg
     6121 cttaagtttt acattatata atcaaagtat ggttttgccc tgccgcaggg gggataaaga
     6181 ctaataaaat gaacattgtt gctggatact ttccaactct cgttgcttca actccagctc
     6241 ctgcctactt aaatccaatt gcttttcaag ctgtagagat tttagcctga gagcaatatg
     6301 ctccttaaaa aataaccaat catctttata atctaaatag cgaagattac gataggcttc
     6361 agcttcactt tggctaaatt ggttatcaac catatctggt aacttattag catcatccaa
     6421 agcattgcta atgccacaac ccaataaaaa attggccgac atcaaagcat ccccaaccaa
     6481 gcaaaacaca cctcccttgg gaaggagcaa agcgaattta tcagcaattt caatatctgt
     6541 tttaaaaaca gaagctaaac caggggcgct catcttaaaa tccgtattgt taaataataa
     6601 ttgcaatacc aagtcaaacc attgttttaa ctctttttta ttattatcct tgagaatgga
     6661 ttcaggaatt tccccagtga gataaacagc tccattcttt tttgaatatt tcaaataaat
     6721 taaaggtgga taatcatgcg tccagcccat ggcctttaac ggagtaatct tatctaaagg
     6781 gatgaagctg gttccctcag ttgggcttaa caccggatta gctatcatcg ccaaaggcgg
     6841 attattgatc gacagataca tcacaccaaa agcattgtga tcaggattat ctaacttttc
     6901 ataaccaatt gcgaactctg gattttgcga attccacaac tgagtaaatg attttgatgt
     6961 ttttccggat gcgtcaacca gcaaatcaaa actgtgattt tgtatgctac cattatgatc
     7021 caaggttaac ttctgattct cagcatcaac cttggcaatt tgcgtgtctc ccaggtgaaa
     7081 aatgagctgc tgctctgcat tattgaattt tacagtataa ggatcttctt cgtcctgaaa
     7141 agtaaaacca cctttgtcat aaatatattt caacttacta tactgataac gctgcaactc
     7201 tgcgatagga attacagtgc gctctttttc aattaattca aaaaaagcta aatccaattg
     7261 atcttctgca ctcaattgca agttggattc atcgatggga cggattaatt tacagataaa
     7321 attatctttg aattccaagg aatacccttt gcttgccagt tgacttagag caaaaaaatc
     7381 tctgatggta tcatccttta agaatacccc ttgtttgcga ctaaaaacat catcttcaga
     7441 tcggcggtca attaattcaa cggaataccc tttaactaat aatgcaagtg cactagccaa
     7501 gccagccggc cccataccaa tgatatatgc tttctttccc ataggttgga ctctactact
     7561 taaataaatg acaaatttta gcatttaatt gttaagaaat tattaggtga gcatttctat
     7621 tgccagtgtt ccgctgtgca ggataaaact cagattgact ctctttttat ataggaagag
     7681 tagcatgttg attattaatt tttctgaact ctgagctggg gggggtccaa tctggaaccc
     7741 ctaatttagg ttcccaaatc gaaacgaatt acatccataa ttcctttttg acaaatatcg
     7801 caaaatgatg cgtgagattc cataatacaa ttcaagcctg gtttatgact tgcaccctga
     7861 tgagatggtg aatctccata gccgccacga tatgccccat attgcggagg ggttgactgc
     7921 cagtcactat agggggtagg aatagacatt tgtggattga ctaactcatt ccattttaat
     7981 ccggaatagc ttggagtagt cagaaaagtg atgttttgtg accacggttc cttcatctca
     8041 ggggcaaatt ccaactcagt ccgtccacca ccttcatatt cttcattgag tccaaaaaaa
     8101 tgacccaatt catgtatcag aatgtaggta aaataagttg aattatcagc aggtattgct
     8161 gtgtgtgatt tgtaattacc tacaccccag tactgactat tattaactaa tactattgga
     8221 taatcgtaag gtgctgacgc caatccctga cgaaacttgt tttctcgtgt aggatatact
     8281 atatgatacc atcgtccaaa attatcccag tatggataat ataaacccag aaaagaatca
     8341 aattctggta ctggtaaacc taagtcttca gcgactccca aacgcttatg agatggatta
     8401 aagactgcat aaaaatccat ataatcgata ccaggaaact gttgactttg taatgcctgc
     8461 acagttttaa tcgctcgatt ccaaaatttc atcttctcac tttgagtata cccttcagca
     8521 tatatattca ctcttaaaga gggagttttc actgcgggta atagttgcct cacttccaaa
     8581 tcattgaaat gacgattatc tcttgttaat tcactagcaa tgatattttt ctcaacaacc
     8641 ttttccatta aacctgtttg cgggttttct gcataaagtt caaagatcat atcatgggag
     8701 ggtacgggga aacgcaaatt aatagcacgt gccagcttgc gatattcctt accagtacca
     8761 atcgcatcat aatagaccgt atcaccaccg aatctgcttt ttaaaactcc caaataactc
     8821 ccccatttgg gtttgtgaat acttcgtgat aataatgccg gtgttccaga ggactctatt
     8881 attactgtac caacctgata agaatctgtt tcttcaaacc aatcggtttg gattcttaat
     8941 acatcaatcc ctgaattttc ctgcacaatc ttcaaagaat caggattgat gtaatgaaac
     9001 tttaaatttg ggtaatgact gtctctgggc actttatagc ggtcccaata gggattggcg
     9061 tatgccagat gggaaaccaa aaaaataaag caaatgataa aaaatcttat catgggtgtg
     9121 acctccttgt attccagaac tactatccca tactaacttg tagattttcc tacaaatgtt
     9181 aaccgagttt aatgaacact cttttatcta tcgaacttga tatttcgtca gattgaacga
     9241 aagcgtgtta atttttgggt atcgtaacaa cgactcacaa aaaatcatga tttataagac
     9301 aaaagagtag aattacgcat gtcacctatc catggaggat tatcacctaa taaaacagac
     9361 ggatcaaaac ctaatgattc gattctaacc cactctttat ccaaatattt caattgatca
     9421 tttaacgctt tgttatctgt tttatttgtt gcatcatagc aacgatcaaa aggatcgtag
     9481 gtatagggat tgcaaactac cgcctccatt ttatccatta tcccaatttt gttataccaa
     9541 tcatcaatca acattggacc ttctggtttg gcagcgggat cgagcacata aatggtaccc
     9601 atgtagttga caacagctga cacatgatac caccaactta ctgttccctc agaagagtat
     9661 ggtgtttgca tttccaaatc accaaaaaca aaaatttttg ctggaataac aaaatgttca
     9721 ctatccagtt taattctgga cagtgctgct ctggcaaagc aaccatcatc aggataaagc
     9781 caactgattc ttctttgaaa atcaggttta tcaggtgaat acaaaaaacg agtgtctcgt
     9841 atcaggttaa acatgttagt catctcgtca taactgctaa cctttggcac ggttgagtaa
     9901 tctattttct gtaaagagac tttctttgaa taaggagttc ccctggcaat taacatcttc
     9961 agttcatttt gtggtttgtt atttatgata cgcttcactg catcctgata agattctcct
    10021 ggaagccgtt tttgtgaggc agttgcgttt gccaagccgc aacaaatcaa aaaaggaaat
    10081 aatgataaaa taatagctat ttttttcata gcaatccttg agtatttata attggttgat
    10141 attttaccaa tttttgtcgt ccttgacatt atttataatt agcatgaact gattgaaaag
    10201 actagtctat tagtcatctg aactatctgt agtcaaaaca taatgactta tatttttaat
    10261 cagggtaagc attcaaaatg ctgaatttaa ttgaactgac cttacaataa gtatctcata
    10321 ataatgatct ataattcaga tatacatcaa tttatagatt aaaggctagt catggctagt
    10381 gatacccctt tttcggactt aagagaaaaa atgttaaatc tctatgatca agcagaaagg
    10441 aaccaattcc gtgaacctgt atttagagac caatttcagg gtgctaagct tgatgatgtt
    10501 actatttctc gagcaagtgt aaaatttcaa tctcagaaga ccagcgcgct catttacaag
    10561 atttattttt agcttattta gaagatcaag catccagcat ttataaagaa tattatgata
    10621 aaaaatttgg ctcaggatct aataacgcca ccagctcatt gcttgaacaa tttgagtcaa
    10681 aaaaagatac aacccttgaa aaaattcgag cggttttgga taaatgtgta acagaaaaca
    10741 aagaagagga ttattggttt actaatgctg taagaggaat ccttgtaact aacgcttttg
    10801 ctatttccga aagctcaagt gaatcctacc aatcagaatt agaacagaga atcagcaaac
    10861 taattccacc tcctggcaat acaaatgagg aaaaataaca aaagatttac agcccctagt
    10921 tgttagatga aataggtatg acttagacac caattcatat taacctaaac ttactattta
    10981 tacggtcttt gtaattagtt atattgcaga gtatccgatt tatcaaaata atatcctgtc
    11041 accaagttga aaccactttt attacagtac ttctttattt tttgttttct acagttatag
    11101 tgggttgaat ttgctcatca tcgtgtatga taccctcagt atcattagta cctttttcaa
    11161 ttaaagtggt atcttcccca gacatttttg ctatcttggc tatattcatt ttcgatgatt
    11221 gctttttgct aaacatgcca tagggatttg ctgaatgaac agggaaatta gttttaatcg
    11281 aatcatcaac atgtatcctt ttggcttcga tacacgacat cactgtatga taagccatat
    11341 taaaatcagg attatttgta tgccaattat actgtttgtc tttaccatca ttgtaataag
    11401 tatattcagg aattactcgt ttctggaaat tacaggctaa ttgatgaata tcgtctctgg
    11461 ttaactcgac tttttgactt ggatccatat taacgatcgc aatatcagta tcagaactta
    11521 agcctagccc atttttacgt actactgcaa gcaaccctgg ttttgactgg attgggataa
    11581 cttcactcca aaaccccata ttggaactcg gcaaggaact taatatacta ttgatttcaa
    11641 acataaattt cctcatatca tgttggccgt tccaaccatt ttgaggtgtt ctgggatccg
    11701 gactataatt ttcgtctgta aaaccaatcg ttatattttt ctgggcgagt ttttcttgga
    11761 atgctttgca aacaactgta tcgccacaat taatttgatt ttgcaaagtc tccagataag
    11821 gaacgagcaa tcgctcttgc gattgtttat catgctgtaa actgttatag atgcttccag
    11881 ctttttcttt ttggatattc tctatagcca tttcggttaa tacctccata gctactttgt
    11941 tgtcgggata ttttttatca aagattatat gagtacgttg tgcataatag gtttggtcca
    12001 gagcgtgaac ccttctgaat acaggttttg ccaataaatc acctgtttca tcacctgata
    12061 gagcatacca cccgccgctt gatgttaaag cacagaaagc tattttttca cgcattcttg
    12121 tttctacttc tttgattgtt gattcattac cgcttagaat ctcattttga atatctttca
    12181 caaaagaata gcgcaggact gactctaaag cttcgtcata atcatagctc ctttcatgtt
    12241 taaatttttc ttgcgcaccg tgcaaagtgt gatgatttgc caaacgatgt tctgccattt
    12301 cttccaatat tgtcatggct aaggagttat gatcatgatt accggagaaa gcaatacatc
    12361 cgcctttggc atggggatgc aatttctttt gtttatccag gaatactact tgagccttaa
    12421 ctccctcctc ctccttacac catccgggta aaccaccata accatctctt tgcgcataat
    12481 aggtaccgcg agtaatatgg gttggccctt tttctggaag caacaaacca tcaaccacat
    12541 cggtaagctt ctgactccca ctgaaaagaa gttcatccaa tataacgggt ttaggtttat
    12601 taaactgctc tgcaagatca taaatataag cataaatttg tttgcgtaat tctggattta
    12661 aatagcctac agcatcaatg cgaacaccct caaagccata atcaatcatg tacttataaa
    12721 tataaggctt ccaaaattgt tcaataattt catctcggat ttgagggtca ctgtagtcaa
    12781 acgcgatagc atccttaaaa tcaggatgga catccttttg aaaccattca ggatgttctt
    12841 ttctatgccg tgaatcggcg gcaacgtgat ttaaaaccaa atcaaacatc ggtaccaaac
    12901 cattattgcg ggcttctgat gtgaacttct gcattgcttc catatccagc tgctttttca
    12961 acttaggatc aacattggga gggagcgggt taaaataagg acttatcaaa tcacaatctg
    13021 tcatagcata tagacttctt gtcacctcat tataagctct gacgccaatt gtcttatcac
    13081 gcttaaataa accctgaaca tcccctgctg tttgtacagg atttatccat acagcattaa
    13141 atcccatggc ctgtaattga ggaagatagt caatcatttc ctctatgctt ccaaattgag
    13201 taggaaacat gttataacag cgtaaaatcc cattaccatc caggattaca ctttcttcac
    13261 gttctgtttt attgactatc tctaattttg attctgccat aataatatgc ccatatcatt
    13321 atcttttaga taaaatatag tacatatctc aggattatgc ctcttgagct taaaaagcaa
    13381 gcaaacatag ctgttgtata atcaataggg aaagttatct tgtaattttt ctcatctttg
    13441 aatgtttttg agcgtctaaa ttgatattga cagtttttaa ataaatcaaa ttgtgttaaa
    13501 atattaccgt atttatatca ccaattatta acaaatattg gtgtcaagac ttagacttta
    13561 ttctctaaca tgtcttgaac ttaaaaattt tgtggaggat ttatgggtag ctcctataat
    13621 gattggctga aatcgtcata tagcgaatta aagaaaataa acatcatcga aaatctgata
    13681 aaagaaaaca attttgccag ggcaaaaatg ctcttaaata atcttgatct
//
LOCUS       DACTJE010000081        13724 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.081, whole genome shotgun sequence.
ACCESSION   DACTJE010000081 DACTJE010000000
VERSION     DACTJE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13724
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.081"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            142..402
                     /locus_tag="I9760_001781"
     CDS             142..402
                     /locus_tag="I9760_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043548.1"
                     /translation="MKILVKSSILAIVLALSTPLFADDTQWMTEHNLVKGVITFQDDS
                     QIQVYAAQADLDKLQQNLANIQSVEVQPNQTDSTTSPNQQSE"
     gene            complement(445..714)
                     /locus_tag="I9760_001782"
     CDS             complement(445..714)
                     /locus_tag="I9760_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HAT5043549.1"
                     /translation="MSLGELLLIFIVAIIVFGPSKLPMLATHLGMLVRKINQLKEHAS
                     SFWQQQLNEIQLYENQRKAEKADQQYQQTDSENQNSDYKKNTRPS"
     gene            complement(711..896)
                     /gene="tatA"
                     /locus_tag="I9760_001783"
     CDS             complement(711..896)
                     /gene="tatA"
                     /locus_tag="I9760_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HAT5043550.1"
                     /translation="MGLSGISPLSLLLILAIIVALFGTSKLKTIGSDLGEAIKNFRKA
                     MNSEETNDTQKDDHKPS"
     gene            complement(898..2547)
                     /gene="ubiB"
                     /locus_tag="I9760_001784"
     CDS             complement(898..2547)
                     /gene="ubiB"
                     /locus_tag="I9760_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAT5043551.1"
                     /translation="MKSIKQLIRLIHINYILAKNGLDNVVVSIKLFAPLRFIVYLNPW
                     NWLRKEKLTRGEALRKSLEELGPIFIKFGQALSTRPDILPEDIAKELSKLQDKVPPFP
                     SHIAMNIIEQAYKQSAYDVFAQFDPIALASASMAQVHAATLKTGENVVVKILRPNMRR
                     LIEQDLSIMYTIAALTDRYWPEGKRFKPKEIVKEFEHTLLDELDLMREAANAAQLRRN
                     FNQSPMLYIPEIYWDYCHHNILVIERIHGIPVTDIVSLRDHGIDIKKLAERGVEIFFT
                     QVFRDCFFHADMHPGNIFVSYQNPKDPQYICVDFGIIGTLTDNDKRYLAENLVAFFNR
                     DYKRVAELHVESGWVSRDTPVAEFESSIRTVCEPIFEKPLKDISFGQVVFRLFQVARR
                     FHMEVQPQLILLQKTLLAIEGLGRQLYPELDLWATAKPFLEKWVREQMGPKAFIKRLK
                     QNLPFFTEQLPHMPKLIFDILEMKKDQLLTVHEFTRLQSENNNQTIQWKSVGLGVFFS
                     FLSLGVISYFDLLDYNHLTTITIAGSVLAGIFVLINRKIRN"
     gene            complement(2637..3254)
                     /locus_tag="I9760_001785"
     CDS             complement(2637..3254)
                     /locus_tag="I9760_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043552.1"
                     /translation="MLKEYSLKALQTAINQAMKLDEQMPQKIQKLDGKTLEMVITPLN
                     VNFYIRFKGSEMQLLHRIDGRPDTIIHSNPIGLIRLSLLPTSKARSLFNDKIRISGDI
                     ELGQSVKKLFDEIDIDWEGHLAHFTGDVVAHQIGSFVRKGLEFKNQFSTSMQQNITEF
                     LQEELRISPSRNELEDFFAEVDELVLSVDRLQAHINNLMSNDEGN"
     gene            complement(3262..4014)
                     /gene="ubiE"
                     /locus_tag="I9760_001786"
     CDS             complement(3262..4014)
                     /gene="ubiE"
                     /locus_tag="I9760_001786"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAT5043553.1"
                     /translation="MTNQKQTTHFGFKSVDWNEKEKKVAEVFHSVAKNYDRMNDLMSL
                     GIHHLWKRYTIELSHVRPGQSVLDLAGGSGDLTRLLSQKVGDSGQVVLADINAAMLHV
                     GRDRLLDEGLFKNIRYVQGNAQCLPFADNSFHCITMGFGLRNVTDKDEALQSMYRVCK
                     PGGKLMVLEFSTPVFPGLKPVYDWYSFNILPKIGKFVANDEASYQYLAESIRMHPDQE
                     TLKAMIERVGFEDCHYHNLSGGIVALHIAYKY"
     gene            complement(4139..4564)
                     /locus_tag="I9760_001787"
     CDS             complement(4139..4564)
                     /locus_tag="I9760_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HAT5043554.1"
                     /translation="MFYGDTVQETRQLFFISWNKYQNKKELSPLEKEIAQVILDHPEY
                     HKVIEQIPSFPEHTYYPELGETNPFLHMGLHLAVREQVTTDRPQGIRTVYTKLIEKYQ
                     DPMAVEHLIMDQLAECLWLSQKNNRPPDENHYLITLQNL"
     gene            complement(4790..5395)
                     /locus_tag="I9760_001788"
     CDS             complement(4790..5395)
                     /locus_tag="I9760_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HAT5043555.1"
                     /translation="MLIYFNGLLLGLSLIMALGPQNVFLIRQGARKNHAALSAVICFF
                     CDVILVCGSVAGLHQLLLAHPNLQIWMVWLGSAFLIYYAIKNLKSALSKNKQTVVETH
                     QPHTRMQIILFALGFSLLNPHAIIDTLVIVGSGSSQYPDHKLAFLAGVISASMIWFSS
                     LTMTTRYFSNLLTQNKVWQKIELLSGILMAYIAMKLITSSL"
     gene            complement(5453..6481)
                     /locus_tag="I9760_001789"
     CDS             complement(5453..6481)
                     /locus_tag="I9760_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAT5043556.1"
                     /translation="MKIFNAMFSKVNGGLEQVFLNYIPALSSQGNLVIPIIHPKAEIR
                     NSCPQDHLITVHNFNQHDFFAIHRLKKLIKIHQPDCIITHSYRAAYLFKKTRTKVPKI
                     AVCHVKGHYDFGTDAIIALTEQMRRDIINSGIEERRVFTIPNLIHIPDGLQYREPKNN
                     QIPIIGVCARFAAIKGVDVFINALAELKKRGVAFQAKIAGDGKEKEQYIELIRHHGLE
                     HEITLLGWIEDKESFYKSIDIFCLPSREESFGLVVLESMMHSLPMVLTRLSGPLEIAG
                     NSGCALFVPPEDPVSMADGLERLLRDKDLSKQLALKAFQRVQHYSSTKVAPMLHKVLE
                     KACEINQQ"
     gene            complement(6544..6897)
                     /locus_tag="I9760_001790"
     CDS             complement(6544..6897)
                     /locus_tag="I9760_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HAT5043557.1"
                     /translation="MSFKPAVELVKLQQNNREVAVIDGQKILIIWHDNQVHAIQSQCP
                     HLKLPLTKGKITESCSIICPFHKSEFDLKTGEAKCWSPWPPVVGNLLGKVSSRKHLKI
                     YPTQIENGQVLVDVG"
     gene            7279..8892
                     /locus_tag="I9760_001791"
     CDS             7279..8892
                     /locus_tag="I9760_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HAT5043558.1"
                     /translation="MGSLVIKKSIIAALVVALGIAGQSVQAESVPDETLQSEKVQTEK
                     VQAEKTETDLFSPVEQIMNPFYPKYPPTKPATGEQAELIKRGEYLAKMGDCIACHTDV
                     EGGTPAYAGGLPINTPFGLFYSPNITPDKETGIGNWTEKDFIRALKDGRDPKGRNYFP
                     VFPYVYFSKITDDDARALYAYFMSIPPVKLKNKSLPFPFGVPGARFALWGWNLLFFFP
                     QENSIEYDPDKSPEWNRGRYIVDTLGHCSMCHTPMNIFGSAKNRYYLTGSFIDGYWAP
                     NITKYGLESASHQEVADVFKAGELINKAGPVAGPMAEVNHNSLSHLTEEDRLAIATYL
                     KTVESEEYLGVSPSDLPPSVARGRQVYLKACLICHKDNKMGAPVIGDSASWYTRLKNS
                     GLTGLYRHAIYGYNQMPVKGACVTCSDNDIISAVDYLLNASLTRSQWQDLASGGVKKY
                     PASGKAVYNENCAVCHNEGKLGAPKIGDKVTWKPLIAQNMDVLIENTIRGKNHLPNGG
                     CNHCSTDEVIEAIKYMVSQSKTEGNYSLW"
     gene            8974..10179
                     /gene="coxB"
                     /locus_tag="I9760_001792"
     CDS             8974..10179
                     /gene="coxB"
                     /locus_tag="I9760_001792"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HAT5043559.1"
                     /translation="MLNRLNICKLIALFSALTSAQSLWAAADEWQLNMYKGVTPLSND
                     MYYLHMVAMAVCAVIGVVVFGVMIYSLIHHRKSKGYQPASFHDNPKLEIIWTIIPFLI
                     LVGLAVPATRVLIRMDDTQLSDVTVKVVGYQWKWQYQYLDQGISYFSSLSTPYEQIEN
                     KQKKNQWYLLEVDNPLVVPVNKKIRFLVTSNDVVHSWWVPELGVKRDAIPGFMHEAWA
                     RIEKPGVYRGQCAELCGINHGFMPIVVKAVSEEEFDRWVSEQTKVEDIYAEKSDKPRE
                     QKTMTRDQLMTLGKQKYDLICSACHQPNGTGLPPMYPPLKGSSVAVGKPISRHIELLL
                     NGVPGSAMQSYKDQLTDEEIAAIVTYERNAWGNNTNDLIQPADVAALRHGTDIKPTMV
                     NKAKAGGLQ"
     gene            10176..11792
                     /gene="ctaD"
                     /locus_tag="I9760_001793"
     CDS             10176..11792
                     /gene="ctaD"
                     /locus_tag="I9760_001793"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HAT5043560.1"
                     /translation="MKYAIAHSEDSHEDHGPEQGKGFMGFIKRWLFTTNHKDIGTLYL
                     WLALISFFVAGAMALVIRLELFQPGHRFVDPNFFNQMTTLHGLVMLFGVVMPAFTGMA
                     NWQIPMMIGAPDMALPRLNNWSFWILPFAFCLLFSTMFHAGGGPNFGWTMYAPLSTTY
                     APPSTDFMIFSIHMMGLSSIMGSINIIATILNLRAPGMTLMKMPLFVWTWLITAFLLI
                     AIMPVLAGAVTMMLADRHFGTSFFNAAGGGDPILFQHVFWFFGHPEVYVLVLPAFGVI
                     SEIIPTFSRKPLFGYHFMVYATVSIAVLSFIVWAHHMFTTGIPLGAELFFMYTTMLIA
                     VPTGIKVFNWVSTMFKGAMTFETPMLFAIAFVFLFTIGGFTGLMLALVPADFQYQDSY
                     FVVAHFHYVLVPGVIFALVAATYYWLPKWTGHMYNERLGKWHFWLSVISVNLTFFPMH
                     FLGLAGMPRRIPDYALQFANFNMIVTVAAFAFGLSQLLFLYNVVATVRKRGTNKDVAT
                     SRVWEGSHGLEWTLPSPPPYHTFTTPPEIK"
     gene            11805..12347
                     /locus_tag="I9760_001794"
     CDS             11805..12347
                     /locus_tag="I9760_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HAT5043561.1"
                     /translation="MIEEKKQKKLLTILTLLVLGMFAFGFALVPIYNSLCKNLGINGK
                     TKMEAVAYDEDKAKIAADREVLVEFVATNNSGVPWAFYPKTKKIKVHPGEIVKLAFYA
                     ENKTDHTMTVQAIPSVTPGIAAKYLKKTECFCFTQQTLNGHEAMNMPLLFHLDTDLPK
                     NVNTVTLSYTLFDVTGRSSN"
     gene            12506..13375
                     /locus_tag="I9760_001795"
     CDS             12506..13375
                     /locus_tag="I9760_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HAT5043562.1"
                     /translation="MGAHGTYYIPKPSHWPLVGSIGLTTTLVGAACWLHHDWYGPYVF
                     IAGLCILVAMMFGWFGQVIYENSKGLYDLQVDRSFRWGMCWFIFSEVCFFGAFFGALF
                     FTRYWSVPILGGEMHPITHYTLWPEFKAIWPLLSNPNNQTFAGAHEAMGAWGLAAINT
                     IILLTSGVTITWAHWALKLRKRTQLLTGMSLTIALGVLFLMLQSYEYHEAYTEMNLTL
                     DAGIYGTTFFMLTGFHGLHVTIGTIMLIVILVRCKKGHFTPERHFAFEGVAWYWHFVD
                     VVWLFLFVFVYWL"
     gene            complement(13398..13670)
                     /locus_tag="I9760_001796"
     CDS             complement(13398..13670)
                     /locus_tag="I9760_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043563.1"
                     /translation="MLIQPIQPVAITIKIGVNNYDCQMISLSESELELKSNDYFEKES
                     QVTFIAKYFRGHAIVRELEFSDYCFTYKMEIVRIQFQPGLLINTRL"
BASE COUNT         3916 a         2643 c         2908 g         4257 t
ORIGIN      
        1 aatgtttttc cattattcaa tttgtttctt gtccatgtcg taactccaag aaaatcattt
       61 aacaacaaaa atagcttata attataatgt acaatatttt tcaactgcat aacggatatc
      121 gatttatgca aggagtgcgt tatgaaaata ttagtcaagt catccatttt ggcaatcgtt
      181 ttggcgcttt caacaccact attcgctgat gatacacaat ggatgacaga gcataatttg
      241 gtgaaagggg ttatcacttt tcaagatgat tcgcaaattc aggtttatgc tgctcaagcc
      301 gatcttgata aattgcaaca gaatcttgct aatattcaat cggttgaagt acaacctaat
      361 caaaccgatt ctacaacgtc accaaatcaa cagagtgaat aacacgggtc ttgtccgagt
      421 taaacgacat gtgctgtttc cagtttagct tgggcgtgtg tttttcttgt agtcagagtt
      481 ttggttttct gaatctgtct gctgatattg ctgatcagcc ttttccgctt tgcgttggtt
      541 ttcatacagc tgaatttcat tgagttgttg ctgccaaaat gaagaagcat gctcttttaa
      601 ttgattgatt tttcgtacga gcatgccaag gtgggttgcc agcatgggaa gctttgatgg
      661 cccaaacaca atgatagcaa caatgaaaat aagtaataac tcaccaagac tcatgatggt
      721 ttatgatcgt ctttttgagt gtcatttgtt tcctcactat tcatggcttt gcggaaattc
      781 ttaatcgctt cgccaaggtc agagcctatt gtttttaatt tggaagtgcc aaacaaggct
      841 acaataatgg ctagaattaa taagagtgat aacggactta ttccacttaa tcccatgtta
      901 gttccttatt ttgcggttga tgagtacaaa aattccagct aaaacagaac cagctatcgt
      961 gatggttgtg agatgattat aatccagcaa atcaaaatag ctaatgacac ctaatgataa
     1021 aaatgagaaa aagacaccca gtcccacact cttccattgg atggtctgat tgttattttc
     1081 agattgcaag cgtgtgaatt catggactgt taacaactga tcctttttca tctccagtat
     1141 atcaaaaatc agtttaggca tatgggggag ttgctcagta aaaaagggca aattttgctt
     1201 taagcgttta atgaatgctt taggtcccat ttgttcgcgc acccattttt caaggaatgg
     1261 ttttgctgta gcccataaat ccaattcagg atagagttgc ctgcccaaac cctctatcgc
     1321 taacagggtt ttttgcagca aaatcagttg gggctgcact tccatatgaa accttctggc
     1381 aacctggaat aaacggaaaa ctacttgccc aaaggaaatg tcttttaatg gcttttcaaa
     1441 aataggttcg caaacggttc ttatgctgct ttcaaattct gcaacagggg tgtcacgcga
     1501 tacccatccg gattctacat gcaattcggc tacgcgcttg tagtcacggt taaaaaaggc
     1561 aaccaggttt tcagccagat aacgcttgtc attatctgtc agagtcccaa tgatgccaaa
     1621 atcaacacaa atatactgag ggtctttggg attttggtag gagacaaaga tatttcccgg
     1681 gtgcatgtct gcatgaaaaa aacaatcccg gaagacttga gtaaagaaaa tttctactcc
     1741 acgctcggcc agttttttaa tgtcaatgcc gtgatctcta agactgacta tatcggtcac
     1801 gggtatgcca tggatgcgtt caatgaccag gatgttatga tggcaataat cccaatagat
     1861 ttcaggtata tataacatcg gagattgatt gaaatttctt cgcaattgag cggcatttgc
     1921 cgcttctctc attaaatcca gttcatccag taaggtatgt tcaaattcct tgactatctc
     1981 tttgggtttg aagcgctttc cctcaggcca atagcggtca gtcaatgcgg cgatggtgta
     2041 catgatactt aaatcttgtt ctataagccg gcgcatattg ggtcttagta tttttacaac
     2101 gacattttcc cctgtcttca gagttgccgc atgcacttgt gccatggaag cggaagccag
     2161 ggcgatcgga tcaaattggg caaaaacatc ataggcagac tgtttatagg cctgttcaat
     2221 gatgttcatg gcgatatggc tggggaatgg aggaactttg tcttgcagtt tacttaattc
     2281 ttttgctatg tcttcaggaa gtatatcggg gcgtgtggaa agggcttgtc cgaatttaat
     2341 gaaaatgggg ccgagttcct ccagcgattt acgcagcgct tcaccacgcg ttaatttctc
     2401 tttgcgaagc cagttccatg ggtttaaata aactataaaa cgtaaaggag caaacagttt
     2461 gatagacaca acaacattat cgagaccatt tttggctaaa atataattga tatgtataag
     2521 acgaataagt tgcttaattg atttcataac agctcatcgg ttgttgattt cgagcttcca
     2581 gccgctcaat atgcaatgat aatccatcca gatcctgaat aaaatcatcc aatcgtttaa
     2641 ttgccttcat cattactcat taaattattg atatgggctt gaaggcgatc aacacttaac
     2701 accaattcat caacctcggc aaaaaaatct tccaattcat ttcttgaagg agaaatgcgt
     2761 aattcttcct gaagaaattc ggtgatgttt tgttgcattg aggtgctgaa ttgattttta
     2821 aattccagtc ctttacgtac aaaagagcca atttgatgag ccaccacatc acctgtgaag
     2881 tgagccaggt gcccttccca atcaatatct atttcgtcaa atagtttctt tacactttgc
     2941 cccaattcaa tgtcgcctga gatgcgtatc ttgtcattaa ataaagagcg ggctttcgat
     3001 gtgggtaata agctcaagcg aatcaatcct atcggattgc tgtgaatgat tgtatcaggg
     3061 cgcccatcaa tgcgatggag caattgcatt tcactccctt taaagcgaat gtaaaaatta
     3121 acatttaagg gggtaatgac catttcaagt gttttgccat ccagtttttg aattttttgc
     3181 ggcatctgct catcgagctt cattgcctga ttgatggctg tttgtaatgc tttcagcgag
     3241 tattctttta acataatcaa ctcaatattt gtaggcgata tgcagagcga ctataccgcc
     3301 acttaaatta tgataatgac aatcctcaaa tccaacccgt tcaatcattg cttttaatgt
     3361 ttcctgatca ggatgcattc tgattgattc tgcaaggtat tgatagctgg cttcatcgtt
     3421 agcaacaaat tttccgattt ttggtaaaat gttaaatgaa taccaatcat aaacaggctt
     3481 aagtccagga aaaactggtg ttgaaaattc aagcaccatt aatttcccac ctggcttgca
     3541 tactctgtac atggattgca gcgcttcgtc tttatcagta acatttctaa gcccaaaccc
     3601 catagtgatg caatgaaaac tgttatccgc aaaagggagg cattgcgcat taccctgaac
     3661 ataacgaatg tttttaaata atccctcatc aagcaagcga tcgcggccaa catggagcat
     3721 ggctgcgttg atgtcggcaa gaacaacctg tccggagtcg cccacttttt gcgataataa
     3781 ccgggttaaa tcaccgctgc ccccggccaa atccaatacg ctctgcccag gtcgaacatg
     3841 acttaattca atggtatagc gtttccagag atgatgaatt cccaaggaca tcagatcatt
     3901 catacgatca taatttttag caacagaatg aaatacttct gctactttct tttctttctc
     3961 attccagtct actgatttaa aaccaaaatg ggtggtttgt ttctgattcg tcatatttgc
     4021 cggatagtat cattaaagtg ttcatcttaa ccgataatca ttaagatacc cagttaaaaa
     4081 ttgtatactt ttttttatcc atgtacagat gaaaacaggg gtatttccag gatacacgct
     4141 acaaattttg caaggttatt aaatagtggt tttcatctgg cggcctattg tttttttggg
     4201 aaagccataa gcattctgcc aattgatcca tgattaaatg ttcaacagcc atgggatcct
     4261 ggtatttttc tattaacttg gtgtaaacag tacggatgcc ttgtggccta tcggtcgtta
     4321 cttgttcgcg aacggcaaga tgaagcccca tatgaaggaa gggattggtt tcgcctaatt
     4381 caggataata agtatgttca ggaaaagatg gaatttgttc aatgacttta tggtattccg
     4441 gatgatcaag aatcacttgg gcaatttctt tttccaaggg agaaagttct tttttattct
     4501 ggtacttatt ccagctgata aaaaatagct gtcgagtttc ttgtactgta tcgccgtaaa
     4561 acataatggc ccgattgata taaaatgatc cattttaact gaataaaaaa gtaacaacaa
     4621 tgttgatgtg caaatatggt aaaaatgttg acattaatca gtggagtatg agatatttat
     4681 tttgcgaggt tggacttgct atgtgtcatt ccactgggtc aattggcgac acactgattg
     4741 gccctttcta ttatcctaaa accctgacat gaactctcta tggcttaatc tataagctgc
     4801 ttgtgattaa tttcatcgca atataagcca ttaaaatacc actaagtaac tctatttttt
     4861 gccatacttt attttgggtt aataggtttg aaaaataacg agtagtcatc gttaatgaac
     4921 tgaaccaaat catgctggca gaaattaccc ctgctaaaaa agccagttta tggtcaggat
     4981 attgactgct gccgctgccc acaatcacca gggtatctat aatggcgtga ggattaagca
     5041 gactaaaccc cagggcaaat aaaatgattt gcattcttgt atgaggctga tgtgtttcta
     5101 caacggtttg tttgtttttg gacaacgcgc tttttaagtt ttttattgca taataaatta
     5161 aaaaggcaga gcctagccat accatccaga tttgcaagtt tggatgagcc aaaagcaatt
     5221 gatgtaatcc agcgacactg ccgcacacca aaatgacatc gcagaaaaag caaatgacgg
     5281 cagagagagc cgcatgattt tttcgcgcac cttgcctgat aagaaaaaca ttttgcggac
     5341 ctaaggccat tatcaatgat aatcccaaga gtaatccatt aaaataaatc aacataattg
     5401 catcaggaca gtaaaaaaaa ggcgattata tcataatttt gcgcaagatg ttttattgtt
     5461 gattgatttc acatgctttc tccaggactt tatgcaacat gggagcaact tttgtgctgg
     5521 agtaatgttg cactctctga aatgctttga gcgcaagttg ttttgacagg tctttgtctc
     5581 gcaagaggcg ttctaaacca tccgccatgc tgacagggtc ttctggggga acaaaaagag
     5641 cacagcctga gttacctgca atttcaagtg ggccggataa gcgagtgaga accatgggca
     5701 aggaatgcat catgctttcc agtacaacca ggccaaaaga ttcttcccta gaaggcaaac
     5761 agaaaatatc aatgctttta taaaatgact ctttatcctc aatccatcca agtaaagtga
     5821 tttcatgctc caaaccatga tgacgaatta attcgatgta ttgttctttc tccttgccgt
     5881 caccagcaat ttttgcttga aaagcaacac ctctcttttt tagttccgcc aaagcgttta
     5941 taaagacatc tactcctttg attgcagcaa atcgggcaca aacaccaatt atcgggatct
     6001 ggttgttttt aggttctcta tattgcaggc cgtctggaat atgaatcaaa ttgggaatgg
     6061 taaatacccg gcgctcctct atccccgaat tgataatatc ccgacgcatt tgctctgtga
     6121 gtgcaataat cgcatcagta ccaaagtcat agtggccttt gacatggcac acagctatct
     6181 tggggacttt ggtcctggtt tttttgaaca aataggctgc tctgtaacta tgggtaatga
     6241 tgcaatccgg ttgatggatt ttaattaatt ttttcaggcg atgaattgca aaaaaatcat
     6301 gctgattgaa attatgcacg gtgattaaat ggtcttgggg gcaactgttc cttatttctg
     6361 ctttgggatg aataattgga atgaccagat tgccttgaga acttaaagct ggaatatagt
     6421 ttaaaaatac ttgttccagg cctccgttta ccttggagaa cattgcatta aatattttca
     6481 ctttaaattt tcctcattca gagctgttcc tctattgata tagaggggta gcaatgggaa
     6541 tggctagcct acatcaacca aaacttgccc attttctatc tgggttgggt atatttttaa
     6601 atgcttccgg gaggacactt tacccaaaag attgccaaca acaggaggcc agggggacca
     6661 gcatttcgct tcgccggttt tcaaatcaaa ttcgctttta tgaaaagggc aaatgataga
     6721 acaggattct gtaatttttc ccttggtcag tggcaatttt aaatgcgggc actgtgactg
     6781 gatggcatgt acttgattat catgccagat aatcagtatt ttttggccat caataaccgc
     6841 gacttctctg ttattttgct gcaatttgac taattctacg gcaggtttga aagacacgtt
     6901 aattacctct atattgaaaa tcagaggatt atatcagtga tgaatttaat ctgttagtag
     6961 aaggctgata aaataaatca gttgtgttat caaaattcct tatttttgct gagttagccc
     7021 agagtatcgt atcaatgacc atacctgaat gatgtctttg agcctgatta tggcatttca
     7081 tgaaatgatg aaatttcgag aaaaggttgt gggatataag gcaggtgaca gctgggttgg
     7141 cataattata atttttaaac agggctgaat ttaaacggtt gaataacgtt gcaacttgat
     7201 aagcgaggag tatacaatgc gacaattatt ctgtctcctc atgagagtcg tgaaaaataa
     7261 gaacaaatag gagggagcgt gggctctttg gttataaaaa aatctattat tgcggcgcta
     7321 gttgttgcac tgggtattgc agggcaatct gtacaagctg agtctgtacc agatgaaacg
     7381 ttacaatctg aaaaagtaca gacggaaaaa gttcaggccg aaaaaacaga aacagatttg
     7441 ttttctcccg tagagcagat catgaatcct ttttatccca aatacccccc gaccaagcca
     7501 gctactggcg agcaagcaga gttgattaaa cgtggtgagt atctggcaaa aatgggtgat
     7561 tgcattgcct gccataccga cgttgaaggg ggtactcccg cttatgctgg tggtttacct
     7621 attaatacgc cttttggctt attctatagc cctaatatta cccccgataa agaaacgggt
     7681 ataggaaatt ggacagaaaa agattttatt cgcgctctta aagatggtcg tgatcctaaa
     7741 ggcagaaatt attttccagt atttccatat gtctattttt ccaagataac tgacgatgat
     7801 gctcgtgctc tgtatgctta ttttatgagc attcctcccg ttaagttaaa gaacaaatca
     7861 ttgccatttc cttttggtgt ccccggggct cgctttgctt tatggggatg gaacttgttg
     7921 tttttcttcc ctcaggaaaa ttccattgaa tatgatcctg ataaatcccc tgaatggaat
     7981 cgtggaaggt atatagtcga tactttaggg cattgcagta tgtgccatac cccaatgaat
     8041 atatttggat cagcgaaaaa cagatactat ctaaccggta gttttatcga tggatattgg
     8101 gctcccaata tcaccaaata tggattggaa tcagcgagcc atcaagaggt agccgatgta
     8161 tttaaggctg gcgaattaat taataaagcg gggcccgtag ctggtcctat ggctgaagtg
     8221 aatcataata gcttgagtca cttgactgaa gaagacaggt tggctattgc aacttatctc
     8281 aagacagtag agagtgaaga ataccttggt gtttctcctt ctgatttgcc tcctagcgtt
     8341 gcacgtggaa gacaagtgta tttgaaggcc tgtctgattt gtcataagga caataaaatg
     8401 ggtgctccag tgattggtga ttcagccagc tggtatacac gcttgaaaaa cagcggttta
     8461 actggtttat atcgacatgc catttatggc tataaccaaa tgccggttaa aggcgcttgt
     8521 gtgacttgtt ccgacaatga cattatttca gcggttgatt atttgctcaa tgcgtccctg
     8581 acccggtctc agtggcaaga tttggcgtct ggcggtgtta aaaaataccc tgccagtgga
     8641 aaggctgtat ataatgaaaa ctgtgctgtt tgccataatg aaggcaaatt gggtgctcct
     8701 aaaataggcg ataaagtgac ctggaagccg ctgatagcac aaaatatgga tgttttgatc
     8761 gaaaacacaa tcagagggaa aaatcatttg ccaaatggtg gttgcaatca ttgttcgaca
     8821 gatgaagtaa ttgaagcgat caaatacatg gtcagtcaat ctaaaaccga aggtaattat
     8881 tctttgtggt agggttacca gcaacatttg aataaatgaa ctagcttaaa cattagtcgg
     8941 tttcccggct acttgaagag atgagggatt gggatgttaa acaggttaaa tatctgtaaa
     9001 ttaattgcgt tatttagtgc cttgacttct gcgcagtcgc tgtgggctgc tgcggatgag
     9061 tggcagttaa acatgtacaa aggggtgact cctttaagca acgatatgta ttacctccat
     9121 atggtcgcta tggctgtttg tgcggttata ggagtggttg tttttggcgt gatgatctac
     9181 tcattaatcc atcacagaaa gtcaaaagga tatcagccag caagttttca tgataatcca
     9241 aaattggaaa tcatctggac tattatcccg tttttaatcc ttgtcgggct tgcagttcca
     9301 gctacccgag tattaatccg tatggatgat acgcagctgt ctgatgtgac ggtcaaggtg
     9361 gtcggctatc agtggaagtg gcagtaccag tatcttgatc agggtattag ttacttcagt
     9421 agcttatcaa caccctatga gcaaattgaa aacaaacaaa agaaaaatca gtggtacttg
     9481 ctggaagtag ataatccctt agtcgtacca gtcaacaaaa aaattcgatt tttagtgaca
     9541 tcgaacgatg tagtccactc atggtgggtg cctgagctgg gcgtcaagcg agacgctatc
     9601 ccgggattta tgcatgaggc atgggccaga attgaaaagc cgggtgtata cagaggtcaa
     9661 tgtgccgagc tttgcggaat taatcatggt tttatgccta tcgtagtgaa agcagttagt
     9721 gaagaagaat tcgatcgatg ggttagtgag caaaccaaag tagaagacat ttatgctgaa
     9781 aagtcggata aaccgcgtga acaaaaaaca atgacccgtg atcagttaat gacgcttggc
     9841 aagcaaaaat atgatctgat ttgctcagct tgtcatcagc ctaatggtac tggcttgcca
     9901 ccgatgtatc ctcccctcaa gggtagttct gttgccgttg gaaagccaat ttcacgacac
     9961 attgaattac tcctcaatgg tgttcctggc tcagcaatgc agtcttataa agatcaactg
    10021 actgatgagg aaatagcagc aattgttacg tatgagcgta atgcttgggg aaataatact
    10081 aatgatttaa ttcaacccgc agatgttgcg gcactgcgac atggtaccga tattaaacct
    10141 accatggtta ataaagctaa agctggaggt ttgcaatgaa atacgcaata gcgcattcag
    10201 aagattctca tgaagatcat ggtcccgaac aaggcaaagg ctttatggga tttatcaaaa
    10261 ggtggctatt tacaaccaac cacaaagaca taggtactct gtatttatgg ttagcactga
    10321 taagcttctt tgttgcagga gcaatggctt tggttatcag gcttgagtta tttcaacctg
    10381 gccatcgatt cgtcgatcct aatttcttta accaaatgac aacattgcat ggattagtca
    10441 tgctgtttgg tgtcgtcatg ccagcgttta ccggtatggc aaactggcaa atccccatga
    10501 tgataggtgc tcccgatatg gctttgccaa gactaaacaa ctggagcttc tggattttac
    10561 catttgcttt ttgtctgttg ttttctacca tgtttcatgc cggtggcggt ccaaattttg
    10621 gctggaccat gtacgcgcct ctctctacaa cttatgcgcc acccagtact gatttcatga
    10681 ttttttccat tcatatgatg ggactctcat ccatcatggg atcaattaat atcatagcca
    10741 cgattttaaa cctgagggct ccgggtatga ccctgatgaa gatgccccta ttcgtctgga
    10801 cctggcttat tacggcgttc cttctgatcg ccatcatgcc tgttctggcg ggtgcagtta
    10861 ctatgatgtt agccgataga cactttggca cttctttctt taatgcggca ggtggaggag
    10921 atcctatcct gtttcaacac gtgttttggt tctttggtca ccctgaagtg tatgttctgg
    10981 tcttgccagc ttttggggtt atttcagaaa taattcccac tttcagccgt aaacccttat
    11041 ttggttatca ttttatggtt tatgccacag tgagcatcgc tgtattatcc tttatagttt
    11101 gggctcacca tatgttcacc acgggaattc ccctgggggc tgaattattc tttatgtaca
    11161 ccaccatgct gattgcggta cctaccggta tcaaggtgtt caactgggtg agtacaatgt
    11221 tcaaaggggc tatgaccttt gaaacgccaa tgctgtttgc catagcgttt gtatttttat
    11281 tcactatagg tggatttaca ggcttaatgt tggcattggt accggccgat tttcaatatc
    11341 aggacagcta ttttgttgtg gctcacttcc attatgttct tgtgcccggc gttatctttg
    11401 ctctggtagc tgcgacttat tattggttac ccaagtggac tggacatatg tacaatgagc
    11461 gtttgggtaa atggcatttt tggctttctg tcatttcagt taatttgact tttttcccca
    11521 tgcattttct ggggctagct ggtatgccaa ggcgaatccc tgactatgct ttgcagtttg
    11581 ccaattttaa tatgattgta acagtggctg cttttgcttt cggtttgtcg caattgttgt
    11641 tcctgtacaa tgtggtagcc acagtaagaa aaagaggcac taataaggat gtagcaacga
    11701 gcagggtttg ggaagggtca catggattgg aatggacttt gccatctcca ccaccttacc
    11761 atacgtttac cactccacct gagattaaat aaaggtggag tttgatgatt gaagaaaaaa
    11821 aacagaaaaa actgctcacc atattaacat tattagtatt agggatgttt gcttttggtt
    11881 ttgctctggt ccctatttat aatagcctat gcaagaatct gggcatcaac ggcaaaacca
    11941 aaatggaggc cgttgcttat gatgaagaca aggcaaaaat tgcagcagat agagaagtct
    12001 tggttgaatt cgtagcaacc aataacagtg gtgtgccttg ggccttctat cccaagacaa
    12061 aaaaaattaa agtgcaccca ggagaaattg taaagctggc gttctatgcg gaaaataaaa
    12121 cagaccatac aatgacagtt caggcaatcc caagtgtgac accaggaatt gcagcaaaat
    12181 atttaaaaaa aacggaatgt ttttgtttta ctcaacaaac gctaaatggc catgaggcaa
    12241 tgaatatgcc tttactattc catttagata cggatttacc gaaaaatgtg aatacggtta
    12301 ctctgtctta tacattattt gatgtgacag gcagatcaag taattaggcg tcgttgatat
    12361 gtctaccttt gcggctggct tgcctcgatt ttctgaacgt tgtttcttga aatcgtgaca
    12421 atgcaattca cgctagccaa atatcaacgg tctttgagta tcagctttgg ctgaatgact
    12481 tattgttaca ggagattaaa gcataatggg agcacacggt acatattata ttcccaaacc
    12541 cagtcactgg cctttggttg gctcaatagg tttgaccact acactggttg gtgcagcatg
    12601 ctggctacat catgactggt atggccctta cgtatttata gcgggcttat gtattttggt
    12661 tgcaatgatg tttggctggt ttggacaagt catctatgaa aacagcaaag gcctatatga
    12721 tttgcaagtt gatcgttcat ttcgctgggg tatgtgctgg ttcatatttt ctgaagtatg
    12781 ctttttcgga gcttttttcg gagccctgtt ttttacaaga tattggtcag ttcctatttt
    12841 gggtggtgag atgcatccta ttactcatta caccttatgg cctgagttca aagcaatttg
    12901 gcctttgctg agtaacccaa ataatcagac ttttgccggg gctcatgaag ccatgggagc
    12961 atggggtctg gctgcaataa acaccattat tttattaact tccggggtaa ccatcacctg
    13021 ggcacattgg gctttaaaat taagaaagcg gacccaatta ctgacaggta tgtcattaac
    13081 tatcgctttg ggcgttttgt tcttgatgct acaaagctat gagtatcatg aagcatatac
    13141 ggaaatgaat ttaacgctgg atgcaggtat ttatggtact acttttttta tgcttacagg
    13201 attccatggt ttgcacgtaa caatcggaac aatcatgctt atcgttattc tggttcgttg
    13261 caagaagggt cattttactc ctgaacggca ttttgctttc gaaggggttg cctggtattg
    13321 gcactttgtg gatgtggtgt ggttgttcct atttgtcttt gtctattggc tatagagcta
    13381 atctaattca tcctttatta caaccgggtg ttgataagca agcccggttg aaattgaata
    13441 cgaacaattt ccattttata agtaaagcag taatctgaaa actctaattc acgaacgatg
    13501 gcatggcctc gaaaatattt tgcaataaaa gtaacctgac tttctttttc aaaataatca
    13561 ttacttttta attccaattc cgattcagac aagctaatca tttgacaatc ataattattc
    13621 acaccaattt ttattgttat agcaactggt tgaataggct gaattaacac ggcttcccct
    13681 aatttctact gaattgtgtg ctaataaatc aaaatgataa aata
//
LOCUS       DACTJE010000082        13356 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.082, whole genome shotgun sequence.
ACCESSION   DACTJE010000082 DACTJE010000000
VERSION     DACTJE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13356
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.082"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            201..488
                     /locus_tag="I9760_001797"
     CDS             201..488
                     /locus_tag="I9760_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAT5043564.1"
                     /translation="MSAVMQQVTQGNDALSHQVISAVKGYLTSVGSKDANLNLYQLIV
                     EEVEAPLFRTVMELTRYNQSKAARVLGVSRGTLRTKLKRYFDDEFIGTRDF"
     gene            599..971
                     /gene="rnpB"
                     /locus_tag="I9760_001798"
     ncRNA           599..971
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="I9760_001798"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1060..1848)
                     /locus_tag="I9760_001799"
     CDS             complement(1060..1848)
                     /locus_tag="I9760_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HAT5043565.1"
                     /translation="MKKLIYISLIFVISNAFAQPAATVLFAKQKVISLREGKEYSLSR
                     GSQLEPGDTVVTGAGALINIKYKNGTLVNIGENSRYKILAFSPNPSDVQIKAELSQGK
                     IKFKTTGKSNESLKTPVIAMAITGTSAEIFVKSDKQTYLNLLEGNINNYAPGSYIATP
                     QGVVAGAFPAEGQVNTPAGTDGSISDAETTETISSESADATDTADATDTADATDTADS
                     AEEASDTIGFIESAQVVDQATTDSIEEEIASEIAEISLECISVI"
     gene            complement(1868..2332)
                     /locus_tag="I9760_001800"
     CDS             complement(1868..2332)
                     /locus_tag="I9760_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043566.1"
                     /translation="MKTIRLFMLSIIAHFLLTCSVIHDVWAEKTLNPEKLANTTITLI
                     SNNQNASIANCSLQSVTSCTILLHLANPGTPGILHVTNHSSFVTATNIEAVLPSSLEF
                     LVDMIESGCDVVPPGSTCSIYFYPVSGPISPQPVQLIGDNIRTATFYLGIEM"
     gene            complement(2372..4618)
                     /locus_tag="I9760_001801"
     CDS             complement(2372..4618)
                     /locus_tag="I9760_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HAT5043567.1"
                     /translation="MNKLTLYFPQFLNHIKWTEFFLGLTVTLLVLLTLFFNIPPLNSL
                     ISQFEGRIYDQIINFNWHSKPENLKVIIIDIDEKSLEQEGRWPWPRSKIAELLNKLQE
                     NGVVTVGFDIVMSEAETNYALGLIEKLSQFSGKMTTDQKQLIATLNSVANQVDNDQTL
                     VNALGKNNAILGFFFHNSEGIRKGSLPTPLRDTDSNPLLANKLSVYQFTNFNGNLAAF
                     TAAATSGGFVTNLPDKDGVVRHALLLANYKNNLYPSLSLAIAMNYLLTDKVALKKYNN
                     QLIGIELDGLQIPTNRKGQILIPFWGGPGTLNYYSATDILNNQFNPEELAGSIALVGS
                     SITLLSDLHHTPVAQLFPGVEIVGNIVKGLVEQKIPREFDWESLQGKLCLVIFGLLFA
                     LVFSSVGIFGKLLIFFITTIVILTNTVILFVFYNNYIPPAFLLTITTLQTFVNFSYSY
                     VIEKRQKRRISELFGQYVPKEYVKELIEHPEHHTMEGLNREMTVLFTDIRNFTTISET
                     LGAGGVKLLLNSFFTPMTKIIFEHRGTIDKYVGDMIVAFWGAPIEDKEHASHAVLCSL
                     SCFEHLPQINTHLLEHGLPQVNIGVGIATGPMNVGDMGSEFRRAYTVLGDTVNLASRL
                     ESLTKFYQVNILVSDKTRSYLDSILWRTVDKVAVKGRKSPLTIYQPLGIVEQASARLL
                     QEQEKYETALAHYYNQDWLAAEIIFNELKNVNPKIYLYQMYLERIAAFKKKRPLPDWD
                     GVYVHRSK"
     gene            complement(4816..5805)
                     /locus_tag="I9760_001802"
     CDS             complement(4816..5805)
                     /locus_tag="I9760_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAT5043568.1"
                     /translation="MKIKFLLSMIGVILSTQSAYAKIPDSSASRAQNINDNKSAKNAN
                     NFLKKITPEFAYSYIDFNFDSTAGLNFNRYNGHSNLYSIGADHFSPVPSIIAGLYVLN
                     IDTEVSSQFQLFPNTPIPSSQTIHNNTLFAHMRKIFTPKFDIDIAGGYGQNRINSQTL
                     LNRAITDVAFSKHTNDNWLASINAVYRKRGKKISLRTYVGMLYSQINSGSYILAFQSN
                     QRGQIIAPLTTKTTYIMEGAEISYKLKPTLTPFVNGGLIQIADFTNSRPILTQQINGS
                     LPQLNMDKNGYKLGAGVIFNYKQMTLRLEEKYYSAGNIFESYQTTAGLEYRFN"
     gene            complement(6009..7442)
                     /gene="gatB"
                     /locus_tag="I9760_001803"
     CDS             complement(6009..7442)
                     /gene="gatB"
                     /locus_tag="I9760_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAT5043569.1"
                     /translation="MEWDTVIGLEVHAQLKTKSKLFSGASTAFGATPNSQTSFIDAGL
                     PGVLPVLNEQAIIMAIQFGLAIHGTINDLSVFERKNYFYPDLPKGYQISQYQKPIVTN
                     GYLNIQLGNNLEKTVHIARAHLEEDAGKSLHDAHTDYTGIDLNRAGTPLLEIVTTPCL
                     YSAEEAINYLKTLHQLVRFLGICDGNMQEGSFRCDVNLSIKPKGSSVLGTRTELKNLN
                     SFRFIEKAIAFEQARHQDILESGLSVIQETRLYNPDNNTTQAMRGKENENDYRYFPDP
                     DLLPIHIDKEQVEEIKNNLPDLPEAISKELKNTPSLNDEDINFILSSPDTYQYYKKIK
                     SLCPAADKTIINWLKGQYAAFLNEHNLTFETPPISAKTMAAFLSKIHEKKISSSIAKN
                     IFSMLCTGEKDIDAIIEREGYQQQNDNSALEEIVEQIIKQYPEQVTEYKAGKEKLLAF
                     FIGQAMKQTKGKANPEQINLLLKKHLG"
     gene            complement(7446..8897)
                     /gene="gatA"
                     /locus_tag="I9760_001804"
     CDS             complement(7446..8897)
                     /gene="gatA"
                     /locus_tag="I9760_001804"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAT5043570.1"
                     /translation="MEHYSLAQLSKALHNREFSSVELTQHCINKIQSNKDLNAFISLD
                     EDQALKEAQSADLVLKSGEGKPLTGIPMALKDLFCTKRLNTTCASKMLANFQAPYDAT
                     IVTKFKQNGAIIIGKTNMDEFAMGSSNENSYFGSVKNPWDRERVSGGSSGGSAAAVAG
                     NLVPFAIGSDTGGSIRQPAAFCGISGIKPTYGLVSRYGMVAFASSLDQAGPFAKSAED
                     LAMILHCMAGFDSKDSTSVDRVIPDYSAEIKNPVDKIRIGLPSCFFQPQVEKGIQDAI
                     HDAVKLFENLGAEIIEIDLKLQPLWVPCYYVIACAEASSNLSRYDGIRFGHRSKSAST
                     LIELITNSRSEGFGNEVKRRILTGTHVLSSGFFDAYYLHAQKVRRLIRDELITTLNSV
                     DVILGPTTPTTAFKLGEKIDDPIQNYLADVFTVAANLAGLPAVSIPTGFENKLPIGLQ
                     LMSKHFSENRLLAIAHHYQQHTNWHLANPNKQR"
     gene            complement(8900..9196)
                     /gene="gatC"
                     /locus_tag="I9760_001805"
     CDS             complement(8900..9196)
                     /gene="gatC"
                     /locus_tag="I9760_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAT5043571.1"
                     /translation="MTLSAKDLENISKLAYLNEDSEHSAKLTQEVSAIMDFVDQLKTI
                     DTSQIAPLFHPLALNQRLRPDEVTEAECLAELEAMAPLFEDNLYLVPKVISSDK"
     gene            9470..11617
                     /locus_tag="I9760_001806"
     CDS             9470..11617
                     /locus_tag="I9760_001806"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAT5043572.1"
                     /translation="MLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASS
                     FEYPGRYTCWDMGFYNPPLVIICQSNTICFEALNQRGEILLSFLYPLLKTTDLWEVIS
                     QTTSFCQLKIKSSDKVFSEEERSHQPSVFSTIRLLLNFFKTDEDSYLGLYGAFGFDLI
                     YQFEQLERCKSRNETQRDMVLYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREG
                     KYSPYQPIHKPEKNCDHEPGEYAQVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSIL
                     FRQMREINPSPYGFFVNLGDEEYLVGASPEMYVRVQGKRVETCPISGTIKRGADAIED
                     AHNIQTLLDSEKEESELTMCTDVDRNDKSRICEAGSVKVIGRRQIEMYSRLIHTVDHV
                     EGVLRDGFDAVDAFLTHMWVVTVTGAPKIWAMNFIEQHEKSPRKWYAGAVGWFGFDGN
                     LNTGLVLRTVRIEKGTAEIRVGATLLYDSIPEAEEEETRLKASAFLDILQKPWQKAKK
                     KIENIPLVGENKKVLLIDHQDSFVHTLANYLRQTGAAVTTVRYDKAIGYLQNNHFDLI
                     VLSPGPGKPVDFKLSETIDMILSKNIPIFGVCLGLQGIVEYFGGVLDVLEYPMHGKSS
                     VIRVLDNKGLFSGLGDCFKAGRYHSLYARAETLPSELLVTAVSEDDVIMAVSHEHLPV
                     HAVQFHPETILSLSDQVGLRIITNLMNMVKDNG"
     gene            11610..12893
                     /gene="clcA"
                     /locus_tag="I9760_001807"
     CDS             11610..12893
                     /gene="clcA"
                     /locus_tag="I9760_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAT5043573.1"
                     /translation="MGKKILILYFVSVLLGILTGAIASLFQLTIQQMDHLLELLFHIT
                     ESYGLPVGIVSAMISMMMLFIAWMMVKYIAPEAAGSGVQEIEGTLLHVRPIFWKRLIP
                     VKFFGGVLSISAKMVMGREGPTIQMGGNLGQMLGELFQLTRRRRDTLIAAGSAAGLAA
                     AFNAPLAGVLFVMEEMRNQFNYSFTNFKMVVICCVMATIVLHLIIGSQPAIQMEVFEL
                     PTLGSLWLFFVFGILMGLAGLLFNVTLMRTVGWLDKQNPKNKKYYILAVGFLVGYLAY
                     SYPATVGGGYVIISEALTMSPAFSVLCSLIVIRFIMTMLSYSSGVPGGIFAPMLALGT
                     LFGLGFFHLFQWLSMDFSIEPGMFAVAGMGALFAAAVRSPITGVVLVVEMTKNYSLIL
                     PLMISCITATIVVQLARNEPIYTQLLERTLRLNAK"
     gene            complement(13067..>13356)
                     /locus_tag="I9760_001808"
     CDS             complement(13067..>13356)
                     /locus_tag="I9760_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAT5043574.1"
                     /translation="KLIQIACLKGSKVECSLALLMQKRLHIIGFVLRSQSIKEKARLW
                     KLAHDHWFNAFENDKIKPVIDSELDLNQIETALQRMQQGSHFGKIVIRVGK"
BASE COUNT         3924 a         2363 c         2712 g         4357 t
ORIGIN      
        1 agaaaaataa atgcaattat ttttacaaac ctaactaaaa tgatacaata atgacttaat
       61 aattccttaa taaatgaata tcattaaaac aatgtttaca ctctttggga tatttgatat
      121 tatatcggtc atggtgttag agccaaatta atgtgctgga catgatttat gttcattaaa
      181 tttgtattag gagattaata atgagtgcag ttatgcaaca agtgacacaa ggtaacgatg
      241 ccttgtcaca tcaagtgatt agtgcagtta aaggttattt aacaagtgtc ggtagtaaag
      301 atgctaattt aaacttatat caattgattg ttgaggaagt agaagcccct ttattccgga
      361 ctgttatgga attaactcgc tataatcaat caaaagctgc tcgagtgctt ggtgtaagtc
      421 gtggtacttt gcgcactaaa ttaaagcgtt attttgatga tgaattcatt ggtactcgtg
      481 atttttaatt cagttatata gctaatactt tccaagttca gttatttttc gcggtattct
      541 aatagaaatt catcgttaat tatggccttt gcctgggcac tctgctaata ttgaagtcag
      601 agtcggtcag gcaatcgctc tttgcctaac agcaagggga ggaaagtccg ggctccatag
      661 ggcagagtgc caggtaactc ctgggaggtg tgagcctacg gaaagtgcca cagaaaatat
      721 accgcccact tttcctttgg gaatcgtagg taagggtgaa atggtgcggt aagagcgcac
      781 cgcgcgattg gtaacagtcg tggcagggaa aaccccactc ggagcaagac caaataggaa
      841 tccatttgac tgcaaggtcc taacgtatgg cccatacggg attcgggtag gtcgcttgag
      901 gcatgtggtg acgcatgtcc cagatgaatg gttgcccacg acagaacccg gcttatcgac
      961 cgactctaat ccatattcta tgttaataca taccattata tgcctttggc tcctatataa
     1021 ataaggaggt aaagtcgcat acaaaaatga catgccaagc tatataacac ttatacactc
     1081 cagggagatc tcggctattt cacttgcaat ttcttcttct attgaatcag tagtcgcttg
     1141 atccactact tgagctgatt caataaatcc aatagtatca gacgcctctt cagcgctatc
     1201 ggcggtatcc gttgcgtcgg ccgtgtctgt agcatctgct gtatctgtag catctgcaga
     1261 ctctgaggag atagtctcag tggtttcagc atcagagatg cttccatctg tgccagctgg
     1321 agtgtttacc tggccttcag caggaaaggc acctgctaca accccctgtg gtgtagcaat
     1381 gtaactccca ggagcataat tatttatatt tccttctagc aaatttaaat aagtttgttt
     1441 atcagatttt acaaatattt cggcagatgt gcctgtaatt gccatcgcta tcactggagt
     1501 tttcaggctt tcatttgatt tgcctgtggt tttaaacttt atttttcctt gagataattc
     1561 agctttaatt tgaacatcag atggatttgg ggaaaaggct agaattttat atctggagtt
     1621 ctcacctata tttaccaagg ttccattttt atatttgatg ttaatcaggg cgcctgcccc
     1681 cgtaacaaca gtgtctcctg gctccaattg agagcctcga gagagtgagt attctttacc
     1741 ttctctgagt gagatgactt tttgtttggc aaataatact gtggctgctg gttgagcaaa
     1801 cgcattgctt ataacaaaaa ttagactaat ataaatcaat tttttcatga gttcttccta
     1861 ctggatatca catctctata ccaagataaa atgtcgcagt tcttatattg tcgccaatta
     1921 actgaactgg ttgcggagaa atagggccac ttacaggata gaaatagatt gagcaggtac
     1981 ttccaggtgg aactacatca caaccgcttt caatcatatc cacaagaaac tctagtgaac
     2041 ttggtagtac agcttcaata ttggttgctg taacaaaaga ggagtggtta gtcacatgga
     2101 gaattcctgg ggtaccaggg ttagcaagat gaagaagtat ggtacaactt gttactgatt
     2161 gaagtgagca attggcaatt gaggcatttt gattattaga aattaatgtg attgtggtat
     2221 ttgccagttt ttcgggattt agggtttttt cagcccaaac gtcatgaatt actgagcagg
     2281 ttaataaaaa atgtgcaatt attgataaca taaacagcct aattgttttc ataatccttc
     2341 ctattgcagt atttaaagtt tcttcttgat cttacttgct tctatgaaca tacaccccat
     2401 cccagtcagg caaaggccgc ttttttttga atgccgctat tctttccaaa tacatttggt
     2461 acaaataaat tttagggttt acatttttta actcattgaa gataatctca gcggcaagcc
     2521 aatcctgatt gtaatagtga gctaatgccg tttcgtattt ttcctgctcc tgcagaagtc
     2581 ttgcagaggc ttgttcaaca atacctagag gttggtaaat agtcaatgga cttttacgac
     2641 ctttgactgc cactttgtca acggtacgcc ataatatgct atccaggtag gagcgagttt
     2701 tgtcacttac caggatattc acttgataga atttggttag actctctagc ctggatgcga
     2761 ggttaacggt atcgccaaga acagtatacg ctcgacgaaa ctcagaaccc atgtcaccaa
     2821 cgttcatagg accagttgct atacctactc caatattaac ttgtggcagc ccatgctcca
     2881 ataaatgggt attaatttga ggcaagtgct caaagcagga aagagaacac aaaacggcgt
     2941 gactcgcatg ctccttgtct tctattggtg ctccccagaa tgccactatc atatctccaa
     3001 catatttatc aatggttcct ctatgctcaa aaatgatttt ggtcattggg gtgaaaaatg
     3061 aatttaatag cagttttaca ccgcctgcac caagtgtttc gctaattgtt gtgaaattgc
     3121 gtatgtcagt aaacagaact gtcatttcac gattcagccc ttccatagta tggtgttcgg
     3181 gatgctctat taattctttg acgtattctt tgggtacgta ttgaccgaat aattcactta
     3241 ttcgtctttt ttgcctcttt tcaatgacat atgaatagga aaaattaaca aaagtttgca
     3301 aggtagtgat ggttaataaa aatgcagggg ggatgtaatt gttgtaaaat acgaacaaga
     3361 taactgtatt tgttaatatt actatggttg ttataaaaaa gattaataat ttgccaaaaa
     3421 taccaacaga agaaaataca agcgcaaata ataagccaaa aatcactaaa caaagttttc
     3481 cttgcaatga ttcccagtca aattctcggg gaattttttg ttccaccagt ccttttacga
     3541 tattacccac tatttcaact ccaggaaata attgggcaac tggtgtatgg tgtaaatcag
     3601 ataagagtgt tatggatgag ccaactaagg cgatactccc agccaattcc tcgggattga
     3661 attggttgtt caatatgtca gtcgctgaat agtaatttag tgttcccggg cctccccaaa
     3721 atggaataag aatttgacct tttctatttg tgggaatttg tagcccatct aattctatac
     3781 ctatcaattg gttgttatat tttttcagag caaccttgtc agttaagagg taattcattg
     3841 ctattgccaa agataaactg ggatagaggt tgtttttata attggccaat aaaagtgcat
     3901 gtcttacaac cccatctttg tcgggtaaat ttgtaacaaa cccgccagaa gttgcagccg
     3961 cagtaaatgc ggccaaatta ccgttgaaat tagtaaattg atacactgac agcttatttg
     4021 ccaggagggg gttgctgtca gtatctctta atggtgtcgg taaagagcct tttctaatgc
     4081 cttcgctatt atgaaaaaaa aaaccaagta tcgcattatt tttgcctaaa gcgttaacaa
     4141 gcgtttgatc attatcaacc tgatttgcaa cgctgtttaa tgtggcaatc aactgttttt
     4201 gatcagttgt catttttcca gaaaattgac tcagtttttc tatgaggccc aaagcataat
     4261 tcgtttccgc ttctgacatg actatatcga aaccaacagt gaccacacca ttttcttgta
     4321 atttatttaa taattcagct attttacttc ttggccatgg ccatcttcct tcttgttcta
     4381 atgatttctc atcaatgtct atgataataa ctttaaggtt ttcaggtttt gaatgccaat
     4441 taaagttaat tatttgatca taaattcttc cttcaaattg ggatattagc gaattgagtg
     4501 ggggaatatt aaagaacaag gttagtaaca ctaaaagggt tactgtcagc cccaagaaaa
     4561 actcagtcca ctttatgtga ttgaggaatt gaggaaaata aagagttaat ttattcacga
     4621 aaattatcct tttgctactc ttaaaccaag atgaaagagc gtcctcttta ttaatcagta
     4681 tatagtcttc gagggaaggg tagaaataat gtttttaagc ctggtagatc acaggtatat
     4741 ttgttccatg ttattgcaaa attaattttc gtgcctccga tatgaagaac aataaataaa
     4801 ttactataca accaactaat taaatcggta ctccagtccg gcagtcgttt gataactttc
     4861 aaaaatgttt cctgcgctgt aatatttttc ttcaagcctt aaagtcattt gtttataatt
     4921 aaaaataact ccagcaccaa gtttgtagcc attcttatcc atattgagct gaggcagaga
     4981 gccattaatt tgttgagtaa gtataggtcg gctattggtg aagtccgcta tttgaatcaa
     5041 accaccgtta acaaaagggg ttaatgtagg cttgagtttg taactgattt cagccccttc
     5101 cataatataa gttgtttttg tagttaaagg agctatgatt tgcccgcgtt ggttggactg
     5161 gaaggctaat atataactac ccgaattaat ttggctgtat aacattccca cataggttct
     5221 tagagatatt ttttttcctc gctttctata aactgcatta atgctggcaa gccagttgtc
     5281 attagtgtgt tttgaaaaag cgacatcagt gatggcgcga tttaacaaag tttgtgaatt
     5341 tattctattt tgaccatatc ctccagcgat atctatatca aattttgggg taaatatttt
     5401 acgcatatgt gcgaataatg tgttgttgtg aatcgtttga ctggaaggaa ttggagtatt
     5461 gggaaacaat tgaaattgag aactcacttc agtatctatg tttagaacat aaagcccggc
     5521 aatgatgctc ggtactggag aaaaatgatc agcacctatg gaataaagat tggaatgccc
     5581 attgtatcga ttgaaattaa gaccagctgt cgaatcaaag ttaaaatcta tatagctgta
     5641 agcaaattca ggcgtgattt tctttaaaaa gttattagcg ttttttgcgc tcttattgtc
     5701 atttatattt tgtgctcttg atgctgagct atcaggaatt ttagcatagg ctgattgtgt
     5761 gcttagtatt actcctatca ttgataacaa aaactttatt ttcattattt caaaaatcct
     5821 tagcgaaatg acaaattaat tagcttgaaa taataccggg aaaaaagtca tgtatgcaat
     5881 taattctata tgaaaattgg ttattataat tgaatgcttg cattatacct tctgacaaaa
     5941 agatgataat agctggcaat caatcaacaa ggccataaat gagcattgtg aattataaaa
     6001 atattgcatc atccaagatg tttttttaat aacagattga tttgctcggg atttgctttc
     6061 cctttagttt gtttcatcgc ttgcccgata aaaaaagcta ataatttctc tttaccggct
     6121 ttgtattctg taacttgttc gggatattgt ttgattattt gctcaactat ttcttccagt
     6181 gccgagttgt cattctgttg ctggtagcct tcgcgttcaa taatcgcatc gatatctttt
     6241 tcacctgtgc aaagcatact gaaaatattt ttagcaatac tagaagagat ttttttctca
     6301 tgaattttgc taagaaaagc agccatggtt ttagctgaaa ttggtggtgt ttcaaaggtt
     6361 aggttgtgtt cattaagaaa ggcggcatat tggcctttta accagttgat aattgtttta
     6421 tcagcagccg gacaaaggga tttaattttt ttgtaatatt gataagtgtc cggtgatgat
     6481 aatatgaaat taatgtcttc atcatttagg gacggagtgt tttttaattc tttggaaatt
     6541 gcttcaggca agtccggcag gttattttta atctcttcaa cttgttcttt gtcaatatga
     6601 attggtaata aatcaggatc aggaaaataa cggtaatcat tttcattttc cttgcctctc
     6661 atcgcctgag ttgtgttatt gtcaggattg taaaggcgag tctcctggat gactgacaga
     6721 ccgctttcca atatatcttg gtgtcttgcc tgttcaaaag caatggcttt ttcaataaaa
     6781 cgaaatgaat tcagattttt aagttctgtt cgtgtgccta aaactgagct gcctttaggt
     6841 tttatagaca gattgacgtc acaccgaaaa gaaccttctt gcatattgcc atcgcatatt
     6901 ccaaggaatc tcactaattg atgcagcgtt tttaaataat taatcgcctc ttcagccgag
     6961 tacaggcaag gggttgtgac tatttccaat agtggggtac ctgctctgtt gaggtcgatc
     7021 ccggtgtaat cagtatgggc atcgtgtagg gatttgccag catcttcttc taaatgagcg
     7081 cgtgcaatat ggactgtttt ttccaggtta ttaccaagct gtatatttaa atagccatta
     7141 gtaacaatgg gtttttgata ctgactgatt tgatagcctt tgggtaagtc aggataaaaa
     7201 taatttttgc gttcaaaaac agacaaatca ttaatggttc catgaattgc caaaccaaat
     7261 tggatagcca ttatgatggc ttgttcattt aacacaggga gaacaccagg caaaccagca
     7321 tcgataaaac tcgtttgcga gttaggggtg gcaccaaaag cagtagatgc tcctgaaaat
     7381 aattttgatt tggtttttaa ttgagcatgt acctcaagac caatgacggt atcccattcc
     7441 atatatcacc tttgcttgtt aggattggct aaatgccagt tggtgtgttg ctgataatga
     7501 tgtgcgattg caagcaagcg gttttcacta aagtgcttgc tcattaattg caaaccaatt
     7561 ggtagtttat tttcaaaacc agttggtata gaaacagctg gtaggcctgc aagattggct
     7621 gctacagtaa atacatctgc caaataattt tgtatgggat catctatttt ttcacccaat
     7681 ttaaaagcgg tggtaggagt tgttggccca aggataacat ctactgaatt taaagttgta
     7741 attaattcat ctcgaatcaa acgcctcact ttttgtgcat gcaaataata ggcatcaaaa
     7801 aaaccggatg agagcacatg agttccagtt agaattcgac gttttacctc gttaccaaaa
     7861 ccttcacttc gagagttagt gattaattca attaaggttg atgcggattt gcttcggtgg
     7921 ccaaatctta tgccatcata acgcgaaaga tttgatgaag cttctgcgca agctataaca
     7981 tagtaacatg gcacccaaag tggctgaagt ttcaagtcga tttcaattat ttcagcccct
     8041 agattctcaa ataatttcac agcatcatgt atggcatcct ggataccttt ttcgacttga
     8101 ggttgaaaaa agcaagaagg aagtcctatg cgtattttgt ctactgggtt tttaatttct
     8161 gctgaataat caggtataac tctatcaact gacgtggaat ctttactatc aaaccccgcc
     8221 atacagtgaa gtatcatggc cagatcttca gctgatttcg caaatggtcc cgcttgatca
     8281 aggcttgagg caaaggcgac cattccgtat cgagagacta atccataggt gggttttatc
     8341 ccgctaatcc cgcaaaaggc tgctggctga cgaattgatc caccggtgtc agagccaatt
     8401 gcaaaaggta ccagatttcc agcaaccgct gctgcagaac cgcctgatga tccacctgac
     8461 actctttctc tatcccacgg gttttttacc gaaccgaagt aactgttttc attagaggat
     8521 cccatggcaa actcatccat attggttttt ccaataataa ttgcgccatt ttgtttaaat
     8581 tttgtgacga tagtggcatc atatggggct tgaaaattag ccagcatttt tgaagcacaa
     8641 gttgtattta atcgctttgt acaaaacaaa tccttgagag ccatggggat accagtcagt
     8701 ggctttccct ccccactttt tagtaccaaa tcagccgatt gcgcttcttt aagagcctgg
     8761 tcttcatcaa ggctgatgaa tgcattcaag tctttattgc tttgaatttt gttaatacag
     8821 tgttgtgtta attcaacact tgaaaattct cgattatgaa gagcctttga taattgagcc
     8881 agtgagtaat gttccattat tatttatccg atgatatcac cttgggaacc aaatataagt
     8941 tatcctcaaa taatggcgcc atggcttcta attcagcaag acattccgct tcagttactt
     9001 catctggtct taagcgttga tttagtgcca aaggatgaaa aagaggtgct atttggcttg
     9061 tatctattgt tttgagctgg tcaacaaaat ccatgatcgc acttacttct tgagtcaatt
     9121 ttgcagaatg ctcagagtct tcatttaaat aagccaattt tgaaatattt tcaagatctt
     9181 tagcagagag agtcatggtc atatccaaat ataagggtta tacattatag cggtattgtt
     9241 ctaaaaatga agtattagtt taattatctt ttagtctgtt gagccaagtt gctttcctag
     9301 agccatgttt aatcatgact gcagtcatgt cttttttgaa gcaagttttg caatctcttc
     9361 tgaagacata aaaaagcaat gatattgaaa tgttacaaga ttcttgcaga acagtggttt
     9421 tacagtagaa tgaatatttg tacgaattaa atgatagaga atggtattca tgttacagca
     9481 atataaaact ctcggtggtg ttcatgttga gttttctcaa caagagttgg attatcaaca
     9541 aggcatagat tttttgcttg agagccttga ttctcaacgt ggggcagtat ttgcttcgag
     9601 ctttgaatat ccaggacgct atacttgttg ggacatgggt ttttataatc ccccattagt
     9661 tatcatctgc caaagtaata ccatctgctt tgaggcatta aatcaacgag gcgagatatt
     9721 attatctttt ctctatccgt tattaaaaac aacagatctc tgggaggtaa ttagccaaac
     9781 gacctcattt tgtcagttaa aaattaaatc atcagacaaa gtatttagtg aggaggagcg
     9841 gagccatcaa ccgtcagtat tcagtacaat tcgcctctta ttgaattttt ttaaaacaga
     9901 tgaagattca tacttgggtt tatatggtgc ttttgggttt gacctgattt atcaatttga
     9961 acagttggag cggtgtaaat caagaaatga aacacagaga gatatggtcc tctatctacc
    10021 tgatgaaatt tatatagtaa atcatcgtaa ggaagaagcc tttgttagac gatatgattt
    10081 tcaatatcag gggcgatcaa ctcaatcttt atccagagaa ggaaagtatt caccatatca
    10141 acctatccat aaaccggaaa aaaactgtga tcacgagcca ggtgaatatg cccaagtggt
    10201 caaaaaagct aaagaaaaat tttcctgtgg tgatttattt gaagtggttc ctagccaaac
    10261 tttctatact cactttgctg aaaagccgtc aattttattt cgccaaatgc gagaaataaa
    10321 tccatcgcca tatggatttt ttgttaattt gggtgacgaa gaatatctgg taggtgcttc
    10381 acctgaaatg tatgttcgtg tccaaggtaa aagagtagaa acttgcccta tctcaggcac
    10441 tatcaaacgg ggagcagatg ctattgagga tgctcacaat attcagacac ttctagattc
    10501 agagaaagaa gaatcagaac taaccatgtg taccgatgtg gatagaaatg ataagtcaag
    10561 aatatgcgaa gcagggagtg tgaaagtcat tgggcgtcgc caaattgaaa tgtattctcg
    10621 attgattcat acagttgatc atgttgaggg tgttttaaga gatggatttg atgcagtaga
    10681 tgctttttta actcatatgt gggttgttac tgtgactggt gcgcctaaaa tttgggctat
    10741 gaatttcatt gaacaacatg agaaatcccc gcgcaaatgg tatgcaggag ccgtaggatg
    10801 gtttggtttt gatggaaatt taaatactgg attggtctta agaacagtgc gaatagaaaa
    10861 gggaactgca gaaattagag taggcgcaac gttactttat gactctattc ctgaagctga
    10921 ggaagaggag acgagattaa aggcttctgc atttttagac attttacaaa aaccatggca
    10981 aaaggcaaaa aagaagatag agaatatccc cctggtaggg gaaaataaaa aagttttact
    11041 tatcgatcat caagattcct ttgttcatac tttggcaaac tatttaaggc aaacgggagc
    11101 tgcggttaca acagttcgct atgacaaggc gattggctat ttacaaaata atcattttga
    11161 tttgatcgta ttatcacctg gccctggaaa accggttgat tttaagcttt cagaaacaat
    11221 tgatatgata ctctcaaaga acataccaat attcggtgtt tgtctggggc tgcaaggaat
    11281 tgttgagtat tttggagggg ttttggatgt tcttgaatac cctatgcatg gaaaatcttc
    11341 agtaattagg gtattagata acaaggggtt gttctccggt ttaggcgatt gttttaaagc
    11401 gggaagatac cattcactct atgccagagc agagacctta cccagtgaat tattagttac
    11461 cgctgtaagt gaagatgatg tgattatggc agtttctcat gagcatttac ctgtgcatgc
    11521 ggttcaattt catccagaga caattttgtc tttatcggat caagtgggtt taagaataat
    11581 caccaatttg atgaatatgg tgaaggacaa tgggtaaaaa aatactgatt ttatattttg
    11641 tatcagttct attagggata ttaacaggcg caattgcttc attattccaa cttactattc
    11701 aacagatgga tcatctgctg gagctattat ttcatatcac agaaagttat ggacttcctg
    11761 taggaatcgt ttctgctatg atatcaatga tgatgttatt tattgcctgg atgatggtga
    11821 agtacatcgc cccagaagca gcaggaagtg gtgtgcaaga aattgaaggc accttgcttc
    11881 atgtgagacc aatattttgg aaacgtctga taccagtaaa attttttggg ggtgtgttat
    11941 ccatatcggc aaaaatggtt atgggaagag aagggcctac tattcagatg ggcggtaacc
    12001 ttggacagat gcttggagag ttgtttcaac ttacacgccg tcgccgtgat actttaatag
    12061 ccgctggttc tgccgctggt ttggctgcag catttaatgc gcctctggct ggtgtactgt
    12121 ttgtcatgga ggaaatgcgt aaccagttta attattcatt taccaatttt aaaatggtcg
    12181 ttatttgttg tgttatggca acgattgttt tgcatcttat tattggctca caaccggcta
    12241 ttcaaatgga ggtttttgaa ctacctacac tgggctcttt gtggctgttc tttgtttttg
    12301 gaatattaat ggggttggct ggtttattat tcaatgtcac actcatgaga actgttggat
    12361 ggttggataa acaaaatccc aaaaacaaaa aatattatat tttagctgtt ggatttttag
    12421 tcggttatct ggcgtactcc tatcctgcta ctgttggggg tggttatgtt atcatcagtg
    12481 aagcactgac tatgtcacct gcatttagcg tgttatgcag tcttattgta atcaggttta
    12541 tcatgaccat gttatcctat tcaagtggcg tacctggcgg tatctttgca ccaatgctgg
    12601 ctttgggaac actatttgga ttaggttttt tccatttatt tcaatggttg agtatggatt
    12661 tttcaattga acctggtatg tttgcagttg caggtatggg agcgcttttc gcagctgcag
    12721 taagatcacc aattactggt gtggtattgg ttgttgaaat gacaaaaaat tactctctaa
    12781 ttctacccct tatgatctcc tgcattactg caacgattgt ggtgcaatta gcacgtaatg
    12841 agccgattta cactcaattg ttagagcgaa ctttacggct caatgcaaaa tgagtctaaa
    12901 tatctcaata tgtattgata gttcagctat ggggaataag gataaaagta agaagagctc
    12961 ttgatttatc taccaaaaac acgaactcac tggacattgc caaggcagct ttggaagagt
    13021 ctgtgcatat aatattgagc tcatgagaaa ttgatgaggc atagcattac ttacctacgc
    13081 gaatgactat ttttccgaaa tgagaacctt gttgcatacg ttgcagcgct gtttctattt
    13141 gatttagatc aagttccgag tcaattactg gttttatctt gtcattttca aatgcattaa
    13201 accaatgatc atgagccaat ttccaaagcc ttgctttttc tttgatgctt tgcgatctga
    13261 gtacaaatcc aatgatgtgc aacctttttt gcataagcaa agccagactg cattctactt
    13321 tgcttccttt gaggcaagca atttggatta atttac
//
LOCUS       DACTJE010000083        13269 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.083, whole genome shotgun sequence.
ACCESSION   DACTJE010000083 DACTJE010000000
VERSION     DACTJE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13269
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.083"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            11..2008
                     /locus_tag="I9760_001809"
     CDS             11..2008
                     /locus_tag="I9760_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAT5043575.1"
                     /translation="MKKKHLIFAYFSQFLLFNLCFFGLQLLLLYSHAESLISSIKLPL
                     PIYFEIIGTLVIQFSLYIIISMIQALLLIGILNRSWHYFSTEQWQIIIWSLFACAILS
                     ANAYYFPLSVFSKLFSPPIPEFIFLILLYFSLACLGLLLLNCLFYRTTARLLSIALPI
                     GICIFLLNNYWKSSLNPNVDSEPNVIILGIDSLSPESVTSRNMPFLHQVLLDSFQFTN
                     TISPLARTYPAWSSILTGLYPKHHLAEENLVERSRVKSQLSIVWDLNKLGYNTIYATD
                     DRRFNSIDENFGFKKIIGPKTGVNDVMLGSYNDFPLGNLLVNFRISSWLFPYNFSNRA
                     SFISYYPETFTNELKRELSLQQNSPVFLAVHFALPHWPYAWAASLPDQVENEFSLSKR
                     DALYLRALKAVDNQFKVIFCYLKKHGYFKNSLVIILSDHGEVLYYPNSRLTSYQKYQS
                     SLTSKLAEYFKAKTATELDKSAGHGSDILSPMQYHSLLAFNVYKDGKLLTKTNKIKTR
                     VALFDLAPTILDFLNITKKQKMDGISLLPTVLNPDTPLPHRTFFIESGMYPNQDFTKE
                     KAIELGKKIYKVNPDTGQLELKPDELIQVNNQKLYGVISGNWILALYPDDNTYIPVIL
                     NLSTGEWIDNLQSDFAKSSPAEKLKRQLQEFYGKKLAYPIQ"
     gene            complement(2245..3711)
                     /locus_tag="I9760_001810"
     CDS             complement(2245..3711)
                     /locus_tag="I9760_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043576.1"
                     /translation="MTQKTLTLPNGFVTDNDRAKARIKYELKQLETQLGKCVKQPDDT
                     AFSKNKHSLYMALADKYSEYLKLIDIDSKFKQFVGVEIPKDISYPEACNRIMHLKQKR
                     SAAVNLLEEAKIIIEISSLYQCLAKAIEPNEVSDVSNHLILNQFDKLKQLFKKENLEL
                     WLSTLVDLWNHNSTLLREPAKLFRNWDLEEQTIALCFFSESEVVNLVNAIFFYKLYPD
                     KLFNELIHPEKLVSVRMRLGFLHEFIELLQKQLHSNALQYGLNPGIDFLFHGEELPQG
                     IMIEVNEVYRDIIQLAVKNLRVQFTPENNEKVTLERLHDLGRAYKFWFNPNRLIDAVM
                     VLQQRLVKESISQEEDLEKFHQEMLVLYRQLTTTECLDLYGYFANNDSRYLLYTLYSI
                     NNGYSLDWLPSLNSSERNAIARVYQALKCVMEALRVELKNRFITTEPYLYDLGKKEIH
                     TGRRNREAVFRIIAIYGCERIVMSDSLERLFNLIEEPN"
     gene            3934..4659
                     /locus_tag="I9760_001811"
     CDS             3934..4659
                     /locus_tag="I9760_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT5043577.1"
                     /translation="MSQRHYLLFIDNEPVSEELKDYFEKFNLDIIQQKKLFPIHVEKG
                     LPTAILINWSILRAEPEVIEQFYNMYPVPLIVINNKPDEESSVAMLEAGADDFMTKPI
                     YPRELHARIGAISRRVMRAQQKLDHGKEVLMFANWRLYPASRQVFGENNEELQLSAGE
                     YDLLLTFVQQPQRVLDREFLLQITKNTDLVPFDRRIDVQISRLRQKIEIDKKKPALIK
                     TIRNGGYMFTARVVTLKESDATL"
     gene            complement(4882..5523)
                     /locus_tag="I9760_001812"
     CDS             complement(4882..5523)
                     /locus_tag="I9760_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043578.1"
                     /translation="MKEQFFQTKDLNTLLRSVEKPIQDLLELNFKTLKTVSYVTPIEL
                     FNVLKPEEVLEKNMNVFIQNSQKAMSYMLNVFQIMEHHWINIYDQMAENPNEKKANPA
                     IDKANRKVDTSTPKSSASKKAVTAKGKTKPAAVQSKAKTSKAKPKISSPIKVKTNTQE
                     RPAAVKQQSQMPLSSLQKDMEKQGINTPVALKNSVAILKNETKDIVPSNNKLK"
     gene            complement(5801..6517)
                     /locus_tag="I9760_001813"
     CDS             complement(5801..6517)
                     /locus_tag="I9760_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAT5043579.1"
                     /translation="MKILYVPFSRHQAGDLKSMVELWKKNDEQFSPDRIEIIYFNDDI
                     NYDQLDEKIEIYICAHGSDDDNVTKLFNHSNPLVAESLDIKEVAERFERDILPISYWI
                     STIHLYCCGTNNKNQMMAELLGHSLLRPEKPIYHYSGSVSIVDEYGKQWSFANHVKIP
                     VHLVAKRIFVLNFFDEEQPHRAFVKKAFQSKTYKELLAKKEDSFFAKVKENRASVLLS
                     KRLGKKSQDGEENSLLRKGN"
     gene            complement(6757..8022)
                     /locus_tag="I9760_001814"
     CDS             complement(6757..8022)
                     /locus_tag="I9760_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAT5043580.1"
                     /translation="MQLPLSTLLVLLFFLICLSAFFSGSEIGMMSINRYKLRHLVKSN
                     DKQAMRVNSMLARPDKLLSVVLIGNTLANIVASTLATLIGQRFYGDAGVAIATALLTI
                     LILVFAEMTPKTLAAIYPQQVAFASSLPLSILQWIFSPLVHLISLITNGILRLFNISV
                     KRMQKETLTGEELRSVVHEAGGLMPVEHKSMLISLLDLEQATVEDIMVPKADIVGIDL
                     DLPWIELLEQLETAQHTRLPIYHGSIDHLAGMIHVRNILNLALENKLDMENLLNSAEV
                     PYFIPEATPLNIQILNFRKMKRRSCFVVDEYGDILGLVTMEDILEEIVGEFTTDIAAL
                     SRDITPQQDGSVIVDASITIRHLNRMMGWSLPLIGPRTLSGLIIEHLGYIPPPDSCLV
                     IDHYHIEILRVSDNMIRGVKMIKTNKKRK"
     gene            complement(8172..8708)
                     /gene="ppa"
                     /locus_tag="I9760_001815"
     CDS             complement(8172..8708)
                     /gene="ppa"
                     /locus_tag="I9760_001815"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAT5043581.1"
                     /translation="MSLMEIQSGRDVPNEVNVIIEIPMHGEPVKYEVDKKTGALFVDR
                     FMTTAMFYPTNYGYIPNTLSEDGDPVDVLVITPVPLISGAVISCRAVGMLKMTDESGV
                     DAKILAVPTTKLSKMYQSMQTYQDIPQHLLLSIEHFFKHYKDLEEGKWVKVEGWVGPD
                     AAREEITSSINRYNHTKK"
     gene            8838..9179
                     /locus_tag="I9760_001816"
     CDS             8838..9179
                     /locus_tag="I9760_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HAT5043582.1"
                     /translation="MNCLFCKIAQGEIPATVVFEDKNILAFRDIRPQAPTHLLIIPKK
                     HIATINDVNDDDSELLANILIRAKKLAQAEGLSEMGYRLVFNVNSGGGQEVYHIHLHL
                     LGGRQMTWPPG"
     gene            9191..9730
                     /gene="folE"
                     /locus_tag="I9760_001817"
     CDS             9191..9730
                     /gene="folE"
                     /locus_tag="I9760_001817"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAT5043583.1"
                     /translation="MEKLYSNLLKELGEDINREGLKDTPGRAANALRYLTKGYNENLD
                     EIINGALFDSDMSEMVIVKDIELYSLCEHHLLPFLGKCHVGYLPDGKVIGLSKIARIV
                     DFYARRLQIQERLTGEIAHCIESTTGARGVAVVIEAKHLCMMMRGVEKQNSVMTTSVM
                     LGEMRNNSSCRLEFLNLIR"
     gene            complement(9789..10880)
                     /locus_tag="I9760_001818"
     CDS             complement(9789..10880)
                     /locus_tag="I9760_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HAT5043584.1"
                     /translation="MKLFSFFLVLISSASLGQSLPLHLLKYPSKFSINIYAAPVSDAR
                     EMALGSKDIVFVGSRSAGKVYAVIPDKKNSHGTRVLTIASGLNMPNGVAFHKGALYVA
                     EIGRILRFDNIESNLNNPPEPVVVTTKLPKEKAHGWRFISFGPDDKLYIGIGAPCNVC
                     LMEDPRYATIMRMNTYGQDLEIYARGIRNTVGFDWDPLHHNLWFTDNGRDWLGDNLPP
                     DELNVAPRKGMDFGFPYCYGKSTVDPVYGKIHSCSEFTPAVFEFPAHVAPLGMSFYTG
                     KLFPSNYWGQIFISEHGSWNRSHKIGYQVTTVKIKNNHVTEVKPFISGWLQGEKAWGR
                     PVDTLVMPDGSLLISDDYANVIYRVSYQQ"
     gene            complement(11015..13204)
                     /gene="pnp"
                     /locus_tag="I9760_001819"
     CDS             complement(11015..13204)
                     /gene="pnp"
                     /locus_tag="I9760_001819"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAT5043585.1"
                     /translation="MAKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVV
                     WKKDGGESNDFFPLTVNYQEKFYAIGKIPGGFNKREGRPSDNETLISRLIDRPIRPLF
                     PDNFFNEVQIIATVLSLNPEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVY
                     LLNPSRKEQEESKLDLVIAGTKDAILMVESEAQELSEDIMRGAMLYGHEMMKNVIKSI
                     EELARDVGKSKPEWKAPEIDTVLKARINDVARNEVEAAYLIKDKQQRYQRLDELREQT
                     ISALLAENDELNADVIANMFGELERSIVRNRILDGEPRIDGRDHRTVRPISIRTKFLE
                     RTHGSCLFTRGETQAIVVATLGNERDAQILDGISGESRDRFMLHYNFPPYSVGETGQV
                     GSPKRREIGHGRLAKRALMAVLPDANEFPYVLRIVSEITESNGSSSMATVCGTSLALM
                     DAGVPLKAPVAGVAMGLIKEGDRYAVLTDILGDEDHLGDMDFKVAGTEKGITALQMDI
                     KISGITNEIMEQALEQALEGRTHILGVMNNALAEHRTELSQHAPRITTMKVAEDKIRT
                     IIGKGGATIKGLIESTGVSIDIDDSGVVQLFSPDKMALEEAQKQIKALIAEIEVGQTY
                     QGKVSKIVDFGAFINLLPGKDGLLHISQICADRTQKVEEVLQEGQEIEVFVAGIDKQG
                     RVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINSGNKISEEEE"
BASE COUNT         4124 a         2704 c         2197 g         4244 t
ORIGIN      
        1 ataatcaatc atgaaaaaaa agcatttgat ttttgcatat tttagccaat tccttttatt
       61 taatctttgc ttttttggct tgcaattgct tctactctat tcgcatgctg agagcctaat
      121 cagttcgatt aagcttcctt tgccaatcta ttttgaaatt atcggcactc tcgtaattca
      181 attcagcctg tatatcatca tttccatgat tcaagctctg ttgcttatag gcattttaaa
      241 tcgatcttgg cattattttt ctactgagca atggcaaatc attatttggt ctttatttgc
      301 gtgcgctata ttaagtgcta atgcctatta tttccctctg agtgttttta gcaaactatt
      361 ttcaccacca attcctgagt ttattttttt aatattactt tatttttctt tggcttgcct
      421 ggggttactc ttattaaatt gcttgtttta tcgcacgact gctcgcctat tatcaatagc
      481 gctacccatt ggcatttgta ttttcctatt aaataattat tggaaatctt ccttaaatcc
      541 taatgttgat tccgaaccca acgtcattat tctgggcata gacagcttaa gtccagaaag
      601 tgtcacatct agaaacatgc cttttcttca tcaagtgcta ctggacagtt ttcaatttac
      661 aaacacgatt agtcctttag ccagaaccta tcccgcttgg agctccattt taactgggtt
      721 ataccctaaa catcatcttg ccgaagaaaa tttagttgag cgaagccggg ttaaaagtca
      781 actcagcatt gtgtgggatt tgaataaatt ggggtataac acaatctatg cgactgatga
      841 tagacgattt aatagcatag atgagaattt tggatttaaa aaaatcatcg gcccaaaaac
      901 aggtgtcaat gatgtcatgt taggctctta taatgatttt cctcttggga atttactggt
      961 taattttcgc attagttcct ggttattccc ctataacttt agtaatagag ccagcttcat
     1021 atcctactat ccggaaacct ttacaaatga attaaaaaga gaactgtctt tacaacaaaa
     1081 ttcccctgtt ttccttgcgg tgcatttcgc tcttccacat tggccttatg cctgggcagc
     1141 atcattgcct gatcaagtgg agaatgaatt cagcctcagc aagcgtgatg cgctttacct
     1201 aagagcacta aaagcagtag acaatcaatt taaagtcatt ttctgctatt taaaaaaaca
     1261 tggctatttt aaaaacagcc tggtcattat actaagcgat catggtgaag tactttatta
     1321 tcccaactca cgattgacaa gttaccaaaa ataccaaagc tcactaacta gcaaactcgc
     1381 agagtatttt aaagccaaaa ctgcaaccga actggataaa agtgctggcc atggttctga
     1441 tatattaagc cctatgcaat atcatagcct tctggcattt aatgtttata aagatggaaa
     1501 gctacttaca aaaacaaaca aaatcaaaac aagagtcgca ttatttgatt tggccccaac
     1561 cattttggac ttcctgaata ttacaaaaaa acagaaaatg gatggcattt cattactacc
     1621 tactgtatta aacccagata ctccactacc acaccgcaca ttctttattg aaagtggaat
     1681 gtatccaaat caagatttta ctaaagaaaa agccattgaa ttaggaaaaa aaatctataa
     1741 ggttaatccg gatactgggc aacttgagtt gaagcctgat gaattaatcc aggtgaataa
     1801 tcaaaagcta tacggagtca ttagtggaaa ctggatttta gccctatatc cagatgacaa
     1861 cacctacatc cccgttattc tcaatctttc aactggagaa tggattgata acctgcagag
     1921 tgattttgcc aaatctagtc cggcagaaaa actaaagaga caacttcaag agttctatgg
     1981 taaaaaactg gcttatccta ttcaataaaa gcgctttata aagctcactg caaagccccc
     2041 atacaaaaca gcagacctct tttattttgc ttcagagcaa ggcagaagca aagtttatat
     2101 gcagtaaatg agcgccgcag aaacgtttgc aatgcagcag taaaatgaaa gaagcaagtt
     2161 ataaaagagg ttgggtatat cccaaaaaac gaattactct tagtcaattg gcacctgaaa
     2221 aattcatatt taccactcaa attgctaatt tggttcttca atgagattaa acaatctttc
     2281 taatgagtca ctcatcacta ttctttcaca accataaatt gcaatgattc taaatacagc
     2341 ctctcgatta cgtcttcctg tatgaatttc ctttttcccc aaatcatata gataaggttc
     2401 ggttgtgata aatctgtttt ttaattcgac tcttagcgcc tccataacgc acttcaacgc
     2461 ttgatacact ctggctatag catttctttc agatgaattt aatgatggca accaatctaa
     2521 agagtaacca ttgtttattg aatataaagt ataaagtaaa tatcgactgt cattgttggc
     2581 aaaataacca tataaatcca gacattctgt tgtggtgagc tgacgatata gaactagcat
     2641 ttcttgatga aatttttcta aatcctcctc ttgagatata ctttccttta ccagacgttg
     2701 ctgcagaacc atcacagcgt caattagcct attaggatta aaccaaaatt tgtatgccct
     2761 tcccaaatca tgcaaacgtt ccagggtaac tttctcatta ttttcaggtg taaattgcac
     2821 tctaaggttt ttaacagcta attggattat atctctataa acctcattca cttcgatcat
     2881 aatgccttgg ggtagttctt caccatgaaa taggaaatca atacctggat ttagcccata
     2941 ttgaagtgca ttgctatgta attgtttttg tagaagttct ataaattcat gcaaaaaacc
     3001 aaggcgcata cgtactgaaa ccaatttttc aggatgaatt aattcattaa ataacttatc
     3061 aggatacaat ttataaaaaa atatagcatt tactaaatta acaacttcag attcagaaaa
     3121 aaaacataag gctatcgttt gttcttcaag atcccaattg cgaaataact tggctggctc
     3181 ccttaataaa gtagaattgt gattccaaag atcaaccaga gttgataacc ataattcaag
     3241 attctctttc ttaaataact gctttagttt atcaaattga ttcaggatta aatgattgct
     3301 tacgtctgaa acctcatttg gctcaattgc tttagcaaga cattgataca atgatgatat
     3361 ctcaatgata attttagctt cctcgagcaa attcactgct gcagaccttt tctgtttcag
     3421 atgcataatc cgattgcaag cttccggata actaatatcc ttaggtatct ccacccccac
     3481 gaattgttta aatttagaat caatatctat taacttcaaa tactccgaat atttatccgc
     3541 caaagccatg taaagtgagt gcttgttctt tgaaaaggca gtatcatccg gctgcttaac
     3601 gcatttgcca agctgagtct ccagctgttt caactcatat ttgattcgcg ccttggctct
     3661 gtcattatcc gtaacgaacc catttggtaa agtaagtgtc ttttgagtca tccctttctc
     3721 ctcaagcatt atcctgacac ttgaacttta acaatagcat tgaaaagaaa tattttccta
     3781 atttttataa cgaacaaaat gcaaattttg caataaacca ggttttgcgc tctaaaaagc
     3841 tctggcaagc ataaatattt aaaaataatt gcaagcaact gataaacttg ccaaaattaa
     3901 agcaatatat tttttgacct ttgaggcaaa ttgatgtcac aacgccacta tttgctattt
     3961 attgataatg agcctgtcag tgaagaactg aaagattact ttgaaaagtt taatcttgat
     4021 attattcaac aaaagaaatt gtttcctatt catgttgaaa aaggattacc tacagccatt
     4081 ttaattaatt ggtcaatttt aagagctgaa ccagaagtaa ttgaacaatt ttataatatg
     4141 tatccagtgc cattaattgt tattaataat aaacctgatg aagaatcaag cgttgccatg
     4201 cttgaagccg gtgctgatga ttttatgacc aaacccattt atccccgtga gctgcatgcg
     4261 cgaattggtg ctatttctcg tcgagtaatg agagcgcaac aaaaactaga ccatggtaaa
     4321 gaagtattaa tgtttgctaa ctggcgactt tatccagcct cccgacaagt gttcggggag
     4381 aataatgaag aattgcaatt aagcgccggt gagtatgatt tgcttcttac ttttgtccaa
     4441 caaccgcaac gagtcctgga tagagaattt ttattacaaa ttaccaaaaa tacagacctg
     4501 gttccttttg accgaaggat tgatgtccaa attagtcgtt tgcgtcaaaa aatcgaaata
     4561 gacaaaaaaa agccagcttt aatcaaaacc attcgcaatg gcggctacat gttcacagct
     4621 cgggttgtaa cccttaaaga aagtgacgcc acattataat tgattattat ctggctgatt
     4681 taagcaaatt cgtcttactt attgagcact ttttaaaaac cattaactga tatttgcttt
     4741 attggcacaa tgcccgccct atcctttact agctagcatt gcaccctatc atgtctagct
     4801 tttgggtatc ccggatttat caagtatcgt ttgctacttc tcaaaaaagc gctcaaaacg
     4861 aaaacaggat aaagcaccag tttatttaag tttgttatta gaaggcacta tatcctttgt
     4921 ttcatttttt aatatcgcaa ctgagttttt caatgcaaca ggcgtattaa taccttgttt
     4981 ttccatatct ttctgcaaag aagataaggg catttgcgat tgttgtttga cagctgcagg
     5041 ccgctcttga gtatttgttt taacttttat tggactgctg attttcggtt tcgctttgct
     5101 tgtttttgct tttgactgta ctgcagcagg ctttgtttta ccttttgctg taacggcttt
     5161 tttactggct gatgattttg gagtactggt atctactttg cgatttgcct tgtctattgc
     5221 tggattagcc ttcttttcat ttgggttttc ggccatctga tcatagatat taatccaatg
     5281 atgttccatt atttgaaata cgttcaacat gtaactcatt gctttttgac tattttgaat
     5341 gaatacattc atattttttt ccaggacttc ttctggtttt aaaacattga ataattcaat
     5401 aggagtaaca taagacacag tttttaatgt tttaaaattg agttctaaca aatcctgaat
     5461 tggtttttca acagatcgaa gcaaagtatt taaatctttt gtctgaaaaa attgctcttt
     5521 cattacactc tccatatgtt gcaatgcaca aaaacaagat tagcttaaaa aaataatttg
     5581 tcaaatatta aatgccattt tcttgtaact ccataaacca gtcccatcgc taactggcct
     5641 ccttttaaat catttttagc ctcttattat aactcttggc tacgacataa tcatatcaaa
     5701 atacttactc ttattctgag ttcaatggtt atcgcacttc acagaatgtc ttttggtata
     5761 acgaattgtc gcaaactata atgtatcaaa ggtaaaaact ttaattaccc tttctcaaca
     5821 aagagttctc ttcaccatcc tggctctttt tgcctaatcg tttggataaa agcactgaag
     5881 ctctattttc tttcactttc gcaaaaaaag aatcttcctt cttggctaat aactctttat
     5941 aagtcttact ttgaaatgct tttttaacaa aagctctatg aggttgctct tcatcaaaaa
     6001 aattcagaac aaatattctt ttagctacca aatgcacagg tattttaaca tggtttgcaa
     6061 aactccattg cttcccatat tcgtcaacaa tagaaactga cccactgtag tgataaatgg
     6121 gcttttctgg tcttaacaat gaatgcccta ataattcagc catcatctga tttttattat
     6181 tggttccgca gcaataaaga tgaatcgttg atatccagta cgatataggt aaaatatctc
     6241 tttcaaagcg ttctgccact tcttttatat cgaggctttc tgcaactaat ggattggaat
     6301 gattaaatag ttttgttaca ttgtcatcat ctgaaccatg agcacaaata tatatctcaa
     6361 ttttttcatc caattgatcg taattaatgt catcattgaa ataaattatt tcaatgcgat
     6421 caggagaaaa ttgctcatcg ttttttttcc aaagttcaac cattgatttc aaatcaccag
     6481 cctgatgcct tgagaaaggt acatataaaa tcttcatctg ttctatttca tattataaag
     6541 gcattatttt aacataaatg aatcaaaaat atatatattg ttattttttt acctttatta
     6601 ttgcctcacc cctcaaaatg agatcttaat atctgcattg aagaaatata ttgcgataaa
     6661 atgaataaat tatatcgctt tatcaatgca taaatattta agtaagctaa aatagggaca
     6721 agctggaagc aaattgatag tcagagtcta taagaactat tttctttttt tattagtctt
     6781 aatcatcttt acacctctta tcatattatc gctgaccctt aatatttcaa tatgataatg
     6841 atcaataacc aggcaagagt cagggggtgg aatataccct aaatgctcga taatcaggcc
     6901 acttagggtt cttggaccga ttaaaggcag gctccagccc atcatgcgat ttaaatggcg
     6961 aatagttatg ctggcatcaa ctatgaccga cccatcttgc tgaggagtta tatcacggct
     7021 taaagcagcg atatctgttg tgaattcacc tactatttct tcaagaatat cttccatagt
     7081 cacaagccca agaatatcgc catattcatc cacaacaaag caacttcttc ttttcatttt
     7141 cctgaaatta aggatttgta tatttaatgg ggtagcttct ggaataaagt aggggacttc
     7201 tgcactattt aacaaattct ccatatccaa cttattttct aatgctaaat ttaaaatatt
     7261 tcttacgtgg atcatccctg ccaaatgatc aatagaacca tgataaattg gtaatcttgt
     7321 gtgttgtgct gtttccagtt gctctaaaag ttctatccag ggtaaatcca aatcaatccc
     7381 aacaatatcg gctttgggaa ccatgatgtc ttcaacagtt gcttgttcca gatccaataa
     7441 actaatcaac atacttttat gctctacagg cattaatcct cccgcttcat gcaccactga
     7501 tcgcaactct tcaccagtta gcgtctcctt ttgcatgcgt tttaccgata tgttaaataa
     7561 tcttaggata ccattggtaa tcaaactgat taagtggacc aaaggagaaa aaatccattg
     7621 taaaatgctc aacggcaatg aggatgcaaa agctacttgc tgagggtaaa tagcagccag
     7681 agttttcgga gtcatttctg caaaaaccag gatcagtata gtcaacaaag cagttgctat
     7741 agccacccct gcatccccat aaaaccgctg cccgattaaa gtggcgagag ttgatgccac
     7801 tatatttgcc aaagtattcc caattaaaac cacacttagt agtttatcag gccgagccag
     7861 catggaatta acccgcatgg cttgcttatc attgcttttt accaggtgtc gtaatttata
     7921 tctattgata gacatcatgc caatttcgga acctgaaaaa aaagcagaca ggcaaattag
     7981 gaaaaacaat aaaaccaata aagtggaaag aggaagttgc actgaatatc cttatacttt
     8041 taagaatgac aaattataca atagaacttg gtgttttgta gtaggggctg aaatgatgcc
     8101 gctattatag ctaaagatat aaggttggtg ctgagcaatg cccaacacct ggcacagttt
     8161 aagctaccgt tttacttttt tgtgtgatta taacggttaa tactggatgt tatttcctca
     8221 cgagctgcat ctgggcctac ccagccctca accttaaccc atttaccttc ttccaggtct
     8281 ttatagtgct taaaaaaatg ttcgatagac aacaataaat gctgaggaat atcttgataa
     8341 gtttgcatgg attgatacat tttacttagt ttggttgttg gaacagccaa aatctttgca
     8401 tcaactccgg attcatctgt catctttaac atgccaacag ctcgacatga aataacagcc
     8461 ccactgatca aagggacagg agttattacc aacacatcca caggatcccc atcttctgaa
     8521 agagtattcg ggatataacc gtaattcgtt ggataaaaca ttgctgtcgt cataaaacga
     8581 tctacaaata aagcaccagt ttttttatca acttcatatt ttacaggttc tccgtgcata
     8641 gggatttcta tgatcacatt cacttcattt ggaacatcac gaccgctctg aatttccatt
     8701 aaactcattt acgcaccctt aataataaat aaaagtaata ttatgcgaaa aatcgcatca
     8761 aaaggcaaag acaagcgttc gcaatataat aaatgaaatg tataatagca ttttgaatct
     8821 aaaacatcgg atgacctatg aattgtttgt tttgcaaaat tgcacagggt gaaattcctg
     8881 caacagtagt ttttgaagac aaaaatattt tggcatttcg cgatattaga cctcaagcac
     8941 caacacattt attgattata cccaaaaagc atattgcaac tatcaatgat gtgaacgatg
     9001 atgatagcga acttcttgct aatattctaa taagagcaaa aaaattagca caggccgaag
     9061 gactaagtga aatgggttat cgattggttt ttaatgttaa ttctggtggc gggcaggaag
     9121 tttaccacat tcatttacat ttacttggcg ggcgtcaaat gacttggccg ccaggttagg
     9181 aattcttgct atggaaaaat tatactcaaa tttgcttaaa gaattaggcg aagatataaa
     9241 tcgggaagga ttaaaggata ctcctggacg agcagctaat gccttacgtt atttaactaa
     9301 aggttacaat gagaaccttg atgaaatcat taacggtgct ctgtttgatt cagacatgag
     9361 tgaaatggtt atcgtcaagg acatagaact gtactcatta tgcgaacatc acttattgcc
     9421 ttttttgggt aaatgccatg ttggatatct acctgatggt aaagtaatag gcttatctaa
     9481 aattgctcgt attgttgatt tttatgcgag gagactacaa attcaagaac ggctgacggg
     9541 cgaaattgct cactgtattg aatccactac cggagctcgc ggtgtcgccg tagtgattga
     9601 agcaaaacat ctttgcatga tgatgcgtgg agttgaaaaa caaaattctg tcatgacaac
     9661 ttcagttatg ctcggtgaaa tgcgtaacaa ttcgagctgc cgtttggaat tcttaaattt
     9721 aattcgttaa tactgataag aaataggtag gggctttatc cccgcctata ataaaataac
     9781 tgctccattt actgctgata gctaacccga tatattacat tagcataatc gtcagagatt
     9841 aatagtgacc catctggcat aacgagcgta tcaacaggtc ttccccatgc tttttccccc
     9901 tgtaaccacc cgctgataaa tggcttaacc tcagttacgt gattattctt aatttttact
     9961 gtagtaactt gatacccgat cttatgacta cgattccagg aaccgtgctc actaataaaa
    10021 atttgtcccc aataattgga agggaacaat ttgcctgtat aaaatgacat accaagtgga
    10081 gcaacatgcg ctgggaattc aaaaacagca ggagtaaact cggaacaaga atgtattttg
    10141 ccataaacag gatctactgt gcttttacca tagcaataag gaaaaccaaa atccatgccc
    10201 tttctcggag ctacattgag ctcatctggg ggtaagttat cacctagcca gtcacgccca
    10261 ttgtctgtaa accaaagatt atgatgaagg ggatcccaat caaaccccac tgtattacgt
    10321 attcctctag catagatctc gagatcttgc ccataggtat tcattctcat aatagtagca
    10381 taacgcggat cttccattaa acaaacattg caaggggcgc caattcctat ataaagctta
    10441 tcatctggtc caaaactaat aaaacgccag ccatgagctt tttctttcgg taattttgta
    10501 gtaaccacaa ctggttcagg aggattattc agatttgatt caatattatc aaagcgtaaa
    10561 atccgcccaa tctcggcgac ataaagtgcg cctttatgaa aggcaacacc atttggcata
    10621 ttcaatccag aagctatggt taaaactcga gtaccatgag agtttttttt atctggaata
    10681 acagcataca ctttcccggc acttcttgag cctacaaaaa ctatatcttt actgcccagt
    10741 gccatttctc gcgcatcaga aacgggagct gcataaatat ttatcgaaaa tttggacgga
    10801 tactttagga gatgtaatgg taaactctgg cctaagctag cagaacttat caatacaaga
    10861 aaaaaagaga atagcttcat gcttaaactc tactttagta gcttataatt tatgcggtca
    10921 tatataccaa ttgcaagcac tcagttaaaa ctcgcgaaaa gctcatgcct tccacgagtt
    10981 tataataaat agaacttgaa atgctactat cttattattc ctcttcttca gagattttgt
    11041 tgcctgaatt aatttcttca gattgctctt ccattgtaag aaacgtagca gataccgggg
    11101 cgtcttcgac ctctttggct tcagcttgcg gcttatcctt ccactccagt ttgactcgcc
    11161 cttgcttatc tatcccagca acaaagacct caatttcctg accttcctgc aatacctctt
    11221 ctaccttttg tgttctgtcg gcacatattt gtgaaatatg cagtaagcca tctttaccag
    11281 gcagtaaatt gataaaagct ccaaagtcaa ctatcttgct tactttccct tgataagtct
    11341 gacccacttc aatttcagca attaaagctt taatttgttt ttgcgcctct tccaaagcca
    11401 tcttatccgg agaaaataat tggacaaccc ctgagtcatc aatatctata gatacgccgg
    11461 tactttcaat aagcccttta atcgttgccc cacctttgcc aataattgtg cggattttat
    11521 cttcagcgac tttcatggtt gtgattcttg gagcgtgctg agataactcg gttctatgct
    11581 cggcaagagc attattcatg acaccaagaa tatgggtacg accttccaat gcttgctcca
    11641 aagcctgttc cataatttca ttggtaatcc cggaaatttt gatatccatc tgcaaagcag
    11701 taatcccttt ttccgtaccg gccactttaa agtccatatc acctaaatga tcctcatcac
    11761 ccaatatgtc tgttaataca gcataacgat caccttcctt gattaagccc atggccacac
    11821 cagcgactgg tgcttttaaa ggtaccccgg catccatcaa tgccagactt gttccacaaa
    11881 cagtagccat agaactggag ccattggatt ccgtaatctc agatacaata cgtagcacat
    11941 aaggaaactc attagcatca ggcagtacag ccattaaagc acgcttggct aaacggccat
    12001 gcccaatttc acgccgttta ggactgccca cttgccctgt ttcgccgaca gaataaggag
    12061 ggaaattata atgaagcata aatcgatctc tgctttcgcc actaattccg tctaatatct
    12121 gtgcatcacg ttcattaccc aaagtggcaa caacaatagc ttgagtttct cctctggtaa
    12181 acaaacagga accatgagtt ctttccaaga atttggtacg aatagaaata ggcctaactg
    12241 ttctgtgatc acgaccatcg atgcgaggct cgccgtcaag aatacgatta cgtactatag
    12301 aacgttctag ctcaccaaac atattggcaa taacatctgc atttaactca tcattttcag
    12361 ccagtaaagc agaaatagtt tgctctctta actcgtctaa tcgctgatac cgttgttgct
    12421 tgtccttaat aagataagcg gcttcaactt cattcctagc tacgtcattg attctagctt
    12481 ttagtactgt atcaatttct ggtgctttcc actcaggttt actcttaccc acgtctctag
    12541 caagttcttc tattgatttt atgacatttt tcatcatttc atggccatat aacatagcac
    12601 cacgcatgat gtcttcactt aattcctgcg cttctgattc caccatcaaa atggcatctt
    12661 ttgttcccgc aataaccaaa tcaagcttag actcttcttg ttcttttcta cttggattga
    12721 gcagataaac gccatcttta tagcctactc gagcggcacc tatagggcca ttaaatggca
    12781 cgccagaaat tgaaagcgct gcggaggctc caatcatagc gataatatca ggagatactt
    12841 cgggatttaa ggacagtaca gtagcaatta tctgtacttc gttaaaaaaa ttatccggga
    12901 ataatggccg gatcggtcta tctattaacc gagaaattaa tgtttcatta tcagatggcc
    12961 gtccttcacg tttgttaaag ccaccaggga ttttacctat ggcatagaac ttttcttgat
    13021 aattcactgt taatgggaaa aaatcattac tttcaccacc atcctttttc caaactacag
    13081 tcactagcac ttgtgtacca ttcatacttg ccattacggc accatcagcc tgtcgtgcta
    13141 cttcacctgt ttccaagatt aatttatggt taccgaatac tatttctttt gtaatttttg
    13201 ccacgtaatg ttccccatta agtaaatata agaaaaaaaa ggcgcagaac gctgcgcctt
    13261 ttattctaa
//
LOCUS       DACTJE010000084        13162 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.084, whole genome shotgun sequence.
ACCESSION   DACTJE010000084 DACTJE010000000
VERSION     DACTJE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13162
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.084"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..280
                     /locus_tag="I9760_001820"
     CDS             <1..280
                     /locus_tag="I9760_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043586.1"
                     /translation="WLIYNFDYLFIWVIYYIREVLQAVLAFLTRIMPFKPYSTSIALI
                     ILLTTVSVGPVLLLDLIYRKRTYKGYAYPYLTSTLILIFCTLILLVVS"
     gene            397..1410
                     /gene="queA"
                     /locus_tag="I9760_001821"
     CDS             397..1410
                     /gene="queA"
                     /locus_tag="I9760_001821"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAT5043587.1"
                     /translation="MNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFR
                     EIADFLQPGDLLVMNDSKVIPARLYGHKATGGKVELLVERITGDFTFLAHIKASKSLK
                     SNDLIYLDAGKRLEVLQRQDDLFLCKACENILDLLNDLGHIPLPPYIAREDESLDKER
                     YQTVYAKCAGSVAAPTAGLHFDDAVLSSIRARGVNIAYVTLHVGAGTFRPVRCERIQD
                     HKMHSEWFTVSPDLCTAVKAAKSMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIF
                     IYPGYQFKVCDGLITNFHLPESTLVMLVSAFIGHQECMALYQEAIDKRYRFFSYGDAS
                     LLL"
     gene            1457..2290
                     /locus_tag="I9760_001822"
     CDS             1457..2290
                     /locus_tag="I9760_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuine tRNA-ribosyltransferase"
                     /protein_id="HAT5043588.1"
                     /translation="MLSTNQNFVPVLNTEAGLCLTAANWQEIKITIASCYLDLLLLKP
                     GYSLLKNITDFTKYLGWSGHLILNASRLVSDKNGMVILISPYDGSRIKLTNSELVHLI
                     LHIKPVAVLLPETIMNGFSGLWEQWDDTILPFLSAKQLETLQVPGKHGVYFNFSEAKY
                     NDDFLSQLQKWSEFPLYVSGPIGADLIEDLNRKKSILIESDEPAKNAMRGIVYGREGN
                     VDLTDESQAFNFQLIDENCSCPTCSAQLTRAYLHHLLANTPLLCQRFLIQHNAYYVQG
                     N"
     gene            2436..2771
                     /gene="yajC"
                     /locus_tag="I9760_001823"
     CDS             2436..2771
                     /gene="yajC"
                     /locus_tag="I9760_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAT5043589.1"
                     /translation="MSFFISDALAAAGNAPAAQTDGTFSLIMIVAIFVLFYFMIIRPQ
                     SKRAKEHRELINRLKKGDEIVTSGGILAKVVSLDDQYIKVSPAEGIEINLQKGAVTAV
                     LPKGTIKSL"
     gene            2793..4649
                     /gene="secD"
                     /locus_tag="I9760_001824"
     CDS             2793..4649
                     /gene="secD"
                     /locus_tag="I9760_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAT5043590.1"
                     /translation="MQNKYPLWKNLMLIVIAVIGFVYAIPNLYTENPVVQISAGTDED
                     TETIKNQVESILKNANMDYKSLVVERDGVEVVFSSTDSQLLARDVIKNSLGSDYTVAL
                     NLAPSTPAWLEAIHADPMKQGLDLRGGVHFLLEVDVESVISRRYEGIMKNIGQELREA
                     GIRYSGIRYLSDKGIELRFRDSDSMENALVELRQKISDVVFVKTKTQNSILGAISPAE
                     LNSIRQNTIEQTMSILRNRVNELGVGEAVVQQQGATRVAVDLPGIQDAARAKQILGGT
                     ATLEFHLVDQENDPFIAKQTGVVPVNDKLYMMDGHPILLKRQVVLSGDSITSAVSSFD
                     QQTASPSVQIQLGGGGESLFTKVTRENIGKRMAIVFVETKTTTQTINGEEKRVTKKEE
                     RVISAPVIQNALGSNFQITGLTDSKEASNLALLLRAGALPAAIYPVEERTVGPTLGKE
                     NIRRGLISLEVGMGLILVLMLVYYHFFGLIADIALMLNLVFLCALLSIIDATLTLPGI
                     AGIVLTVGMAVDANVLIYERIREELRNGLSPQAAIHAGYERAFSTILDANVTTLIVAI
                     ILFAVGTGPVRGFAVVLSLGLLTSMLTGITYTRAIVNWYYGGRNVKKLSIGI"
     gene            4662..5579
                     /gene="secF"
                     /locus_tag="I9760_001825"
     CDS             4662..5579
                     /gene="secF"
                     /locus_tag="I9760_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAT5043591.1"
                     /translation="MEFFNPNSKIDFMGARRWTALFSILIFVVSIGALMINGLKWGLD
                     FTGGTQIEISYSKAADLPLIRENLNKAGFKEAQVVSYGTSKDVLISIAPRADKQQSML
                     VDQVMEVLPGAVKQRVDFVGPQVGQELATKGALAIIVSLLATMIYIAMRFEYKLAVSS
                     AVALIHDPVLILGVFALFGIEFDLKALAGLLAVIGYSLNDTIVVFDRVRENFVKIRRT
                     SPIDIMNISINQTLSRTIMTSVLTLFVVVALFVYGGEAIYGFSLALIIGIVIGTYSSI
                     YVAGALAVAMGLDNKDFLPSHRREVDDRP"
     gene            complement(5752..6093)
                     /locus_tag="I9760_001826"
     CDS             complement(5752..6093)
                     /locus_tag="I9760_001826"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HAT5043592.1"
                     /translation="MTSDLSSKHCESCEGIGAALNSEQIKNLLPQLNTKWEVTEDNRI
                     IKRAFSFKNFYETMAFVNAIAWIANIENHHPDLEVGYNYCRVHFMTHALNGLTHNDFI
                     CAAKIDKLLVD"
     gene            complement(6130..7239)
                     /locus_tag="I9760_001827"
     CDS             complement(6130..7239)
                     /locus_tag="I9760_001827"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAT5043593.1"
                     /translation="MSIDFQQLPHAGIRSLIPYVPGKSIEELAKEKGITDIIKLASNE
                     NPLGCSPLALSVIQTMSSHYIATYPSPWNHPLMSKLASYLKVKPEQLFLSNGSDYLFN
                     ILLNCFALHTDRHILTHDYAFSTYAIQANSLQIPINSVPIGHNWEVNITDIVNACNQQ
                     TGIIFIANPNNPTGVLIQQEEIKYLLEQIPKSTLLVLDEAYYEFAASQLTVNSLDWLE
                     EHPNLVVTRTFSKIYGMAGLRLGYAIANPSIINILKRVQLPFIVNQVALAAAYAAIDD
                     DDFIQSSLKMNNEGMLQLQAGFNELNIKYLPSSCNFLTFDCEEDSMALYNYLLDNGII
                     VRPLHAYKMNNFIRVTIGTKEQNSRFLTALKNFYL"
     gene            7399..7713
                     /locus_tag="I9760_001828"
     CDS             7399..7713
                     /locus_tag="I9760_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAT5043594.1"
                     /translation="MSKHQGFTLMEVLVSLMLMTTVVLMLVEQQLKTRDIINHLIWRI
                     QGSQYLDQVDEALYISENRLPLPPSNYHFDIRRSQKKIILNVTWFKKSQSIIRSYDLD
                     AH"
     gene            7652..8458
                     /locus_tag="I9760_001829"
     CDS             7652..8458
                     /locus_tag="I9760_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043595.1"
                     /translation="MLRGLKKASQLFAPMIWMLIEMGRQTGFSLSEMLISLFLASIIT
                     ITLTQFYIRSKSQYLKTQEQLEINFDLLWIGDLLSDSIRKAGFTPCVSVEKLKTFDSR
                     HAERKIINFQIENMPQVQLRINRMSDVYTEVANFMSPTQIVIEESVISNIKHPLIISD
                     CRQGEVHTVSKIDKLSNGYQITLKKPMQFRYDEITYLGEWLEETWFTKQNEKGEKTLH
                     YKLFQTEELSPFIHEFYVTRRLIKQRSVLDFSIGSDKETLRKISVSVRNL"
     gene            8455..9012
                     /locus_tag="I9760_001830"
     CDS             8455..9012
                     /locus_tag="I9760_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043596.1"
                     /translation="MKKTDSGFILLMTLCITFLMSLLVLASLQQIFLQFKALNSQETF
                     HQNFYQLEHWMMQLARSPLLYAGMDCYLQKDYGANKIANELVNNKGCVLILNQKKYRY
                     FIEDLNEFSCLVLNKDGQKYASHHFRFTVLQDEEHGESTVMQLRFIKLGSNLSSCPEG
                     EIQVKEGVSSWRYLPDYKNFEILTG"
     gene            9122..10315
                     /locus_tag="I9760_001831"
     CDS             9122..10315
                     /locus_tag="I9760_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAT5043597.1"
                     /translation="MKSKLIYLTVALAAIILSAAVWWLQSPKPTFRQVSFKHLPGWEN
                     AQLKKSLETFQTSCRAFLKQNPERVVGTEQIDLQAKDWHPACRAALKINPVDEQSAKV
                     FFEKWFAPVEFYDDKPVKGLFTGYYMPLIKGSYTKSKEFSVPIYELPSNLVTVDLGLF
                     LPNLKNKKIIGRIAGNKVVPFYTREQINKGAIKDTARVLVWINSPVDRLFLEIQGSGI
                     IELEDGKRIFIGYDGQNGAPYTAIAGVLIKKGIMTKHNASMQRIKKYLEAHPKQMDKI
                     INQNKSFVFFRKLSLEAALGSQGVALTPGYSLAIDKQWIPMGAPLWLNTTRPDSKNPD
                     VNKPMQRLMIAQDTGGAIRGKVRGDVFWGGGDKATLIAGHMKNEGHYWLLLPAHALSR
                     LAQLK"
     gene            complement(10432..11292)
                     /locus_tag="I9760_001832"
     CDS             complement(10432..11292)
                     /locus_tag="I9760_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAT5043598.1"
                     /translation="MLKRLLGVASLFLILNHQCFAEDHEIAITIDDLPFVGSGTNTPG
                     NLKRTQERFMAIVNTLVDNQVPATGFAIGGAIAKNEWELLEIFRNQGFSIGNHTYKHR
                     SLNSMTAENYIADIEKADTVLSPVMTEPKYFRYPYLAEGSGEKKQKVHEWLAAHQYTI
                     APVTIDSKDYEFNAQFYRIPYRQRPQRLAQFKKRYLAFIWQQTLRAEKKVKKVEGQPV
                     KHILLIHANLINSLCLADIIEMYRSNGYKFITLQEALKGNTATPVNDSSPTEALKSET
                     EKKEINEPQS"
     gene            11784..12284
                     /locus_tag="I9760_001833"
     CDS             11784..12284
                     /locus_tag="I9760_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043599.1"
                     /translation="MTKEKSESGNSPSNSEEHGTLGPDVYENARKLNIQLATRGPAIL
                     YALGKASEQVTNGNVVTAVGGMVVGAVGFFGNRFYRNKGDDSAYTYMGQIVGAVQFTA
                     GFTLLALQAFEVFTDTGETSPLLNVVKLSGVALTLTESALILINSYLYGDGSAKPTSI
                     TSPKID"
     gene            12364..13014
                     /locus_tag="I9760_001834"
     CDS             12364..13014
                     /locus_tag="I9760_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAT5043600.1"
                     /translation="MTYSFSKPVSWVFLFTAVIYLVSLSFIQYPATTVLKPIPIVCLI
                     VGVFRTSLSSSAKILLILALVFSLAGDVVLTLPFSLQLELGIACFLLAHCFYITLFLK
                     SFEFNRLHLFYYLPIFLFMGFAAFTMIPYLGNLLIPVMIYFCVLMLMVFSAFQVKKET
                     LTISSGALFFLISDLTLALNLFIYTQADVRIFVMFTYYVAQFLLTFGLVRLYEKGG"
BASE COUNT         4010 a         2160 c         2707 g         4285 t
ORIGIN      
        1 ctggctaatt tataattttg attatttatt tatctgggta atttattata ttcgagaagt
       61 attgcaagct gtgttagctt ttttgacgcg gattatgcct tttaagccat attccacatc
      121 gatagcactt attatactac tcactactgt ttcagtggga cctgtattat tactggacct
      181 aatctaccgc aaacgaactt acaagggtta tgcttatccc tatttaacaa gcacactgat
      241 tttaattttc tgtacgctga tcttgctcgt agtctcttaa acagataaaa aaaacaaatg
      301 atgttaattt tctaataaaa tgaataaatt cctgataatt gattttaaga ctaaagttcg
      361 gtgtataatt gcaccaattt gtttttaggt tgttctatga ataagcaaga tttttatttt
      421 gatttgcctt ccgaattaat agcccaatat cctctggcga atagaagtga ttcacggctt
      481 ttaatttata atcgacagac agaagaatat ggccattatc aattccggga aattgcagat
      541 ttcttacagc caggggactt attggtaatg aatgacagca aagtgatacc agcacgctta
      601 tacggccaca aagccactgg cggtaaggtt gaactgttag tagagaggat aactggcgat
      661 tttactttcc tggcccatat caaagcgagc aaatcattaa aatcaaacga tctcatttat
      721 cttgatgctg gtaagcggct tgaagtatta caaaggcagg atgatttatt tctttgtaag
      781 gcgtgcgaaa atattttaga tcttttaaat gatcttgggc atattccttt accaccttat
      841 atcgccagag aagatgaatc actggataag gagagatatc aaaccgtcta tgccaagtgc
      901 gcggggtctg ttgctgcgcc tacagcagga ttgcattttg atgatgctgt tttatccagt
      961 attagagcgc gaggagttaa tattgcttat gtcactctcc atgtaggggc aggaacattc
     1021 aggccagttc gctgtgaacg cattcaggat cataaaatgc atagtgagtg gtttacggtt
     1081 agtcctgatc tgtgcactgc agtgaaggct gccaaatcta tgggaaatag agtaattgct
     1141 gttgggacta ctgcattgcg cagccttgaa agcgctgcta tgggaggaga gctaattcca
     1201 tgtagtcgtg ataccgatat tttcatttat cctggctacc agtttaaagt ttgtgatgga
     1261 ttaataacta attttcattt gcctgaatca actctggtga tgttggtgtc ggcttttatt
     1321 gggcaccagg agtgtatggc tctttatcaa gaagcaatcg ataaaaggta tcgttttttt
     1381 agttatggag atgccagttt attgttataa agtgctctgg aatataataa ttttggctac
     1441 agttagaggg tagtggatgt tgagtacaaa tcaaaatttt gttcctgttt taaatactga
     1501 agcaggacta tgtcttactg cggccaattg gcaagaaatt aagattacta ttgcatcttg
     1561 ttatctggat ttattgttat taaagccagg atattcatta ttgaaaaata ttactgattt
     1621 caccaaatat ctagggtggt caggtcatct catcctcaat gcaagccgcc ttgtatccga
     1681 taaaaacggg atggtgatat tgatttcacc ttatgacgga agtcgaatca aattaactaa
     1741 ctcagagtta gtgcatttga ttttgcatat taagcctgtc gcagtgcttt tacctgaaac
     1801 gattatgaat ggtttttccg ggctatggga gcaatgggat gatactatat tacctttttt
     1861 gtcagcgaag caacttgaaa ccttgcaggt gcctggaaaa catggtgtgt atttcaattt
     1921 cagtgaagca aaatataatg atgatttctt gagtcaattg caaaaatggt ctgaatttcc
     1981 tctttatgtc tctggtccta tcggagctga tttaatcgag gatttaaaca ggaagaaatc
     2041 gattttaatc gaatcggatg agcctgcaaa aaatgctatg cggggcattg tatacggccg
     2101 cgaagggaat gttgatttaa cggatgaaag ccaggctttc aattttcaac tgattgatga
     2161 gaattgctca tgtcctactt gttcagcaca gttgacaaga gcttatctgc atcatttgtt
     2221 agctaatacc ccattgctat gccagagatt cttgattcaa cataatgcgt attacgttca
     2281 aggtaattaa ctattcattt accttcgagt aaattcgcta tatgatgtca cgctggtaat
     2341 ccatacaaga gttcttagaa ctatcttttg aaattggttt atcgaccatc ttcgaaagaa
     2401 aaccaggaaa ttaatacccg atttgaggag attttatgag tttttttatt agtgatgcat
     2461 tagctgcagc aggaaatgcg ccagctgctc aaactgatgg aactttttca ctgattatga
     2521 tcgttgcgat ttttgtcttg ttttatttta tgataataag gccgcaaagc aaaagagcca
     2581 aagagcatag agaattgata aatcgcttaa aaaaggggga tgaaatagta acatccggag
     2641 ggattttggc aaaagttgtg agcctggatg atcaatacat caaagtaagc ccagcagaag
     2701 gtatagaaat taatttgcaa aaaggcgcag ttactgcagt attaccaaaa ggcacaatca
     2761 aatcacttta agttaggcat agggaatcag aaatgcaaaa taaatatcct ctatggaaaa
     2821 atctaatgct gatagtgatt gcagtcattg gatttgttta cgctataccg aatttgtata
     2881 cggaaaatcc cgttgttcaa atttctgcgg gtacagatga agatacagag acaataaaaa
     2941 atcaggtaga gtccatatta aaaaatgcca atatggatta taaatctctg gttgtggaga
     3001 gggatggggt agaggtagta ttttcttcta ctgattctca attattagct cgtgatgtta
     3061 tcaagaatag cctgggatct gattacacag tggctttgaa tttagcgcct tccacaccag
     3121 cttggcttga agcaattcat gctgatccta tgaaacaagg attggactta agaggcggtg
     3181 ttcattttct tcttgaagtg gatgtcgaga gtgttataag tcggcgttat gaaggaataa
     3241 tgaaaaatat tggccaagaa ttacgtgaag caggtattcg ttattcaggc atacgttatt
     3301 tatcagataa agggattgaa ttgcgtttca gagattctga ctcaatggaa aatgctttag
     3361 ttgagttaag acaaaaaata tctgatgtgg tttttgttaa aactaaaaca caaaattcaa
     3421 ttttaggcgc catttctcca gcagaattaa attccataag acaaaatact attgagcaga
     3481 caatgagtat cttgagaaat cgtgtcaacg agctcggagt tggtgaagct gtcgtacaac
     3541 aacaaggggc gactcgagtc gccgttgatt tgccaggcat tcaagacgcg gccagagcaa
     3601 aacaaatact tggtggtact gctactttgg aatttcattt ggttgatcag gaaaatgacc
     3661 ctttcattgc aaagcaaact ggcgtagttc ctgtcaatga taagctgtat atgatggatg
     3721 gtcatcctat cttattaaag agacaggttg ttttaagtgg tgactctatt acgtcagcag
     3781 tgtccagttt tgatcaacaa acggcttctc catctgttca aattcagttg ggtggtgggg
     3841 gcgagagctt gtttactaaa gttacgcgtg aaaatatcgg taagcgcatg gcgatcgttt
     3901 ttgtggaaac caaaacgact acacaaacaa ttaatggcga ggaaaaacga gtaacaaaga
     3961 aagaagagcg agttattagt gcgccagtga ttcaaaatgc tttaggtagc aatttccaga
     4021 ttaccggact gaccgatagt aaagaagcaa gtaatctggc tttattatta agagctggcg
     4081 ctttacccgc tgcaatctat cctgttgaag agcgtaccgt tggcccaact cttggaaagg
     4141 aaaatattcg ccgaggactt atttccctgg aagttggtat gggtttaatt ttggtactaa
     4201 tgctcgtgta ttatcatttt tttggtttaa ttgctgatat tgcattgatg ctgaatttag
     4261 tttttctttg cgcattgtta tccataattg atgcgactct gactttaccg ggaattgcgg
     4321 ggattgtttt gaccgtgggc atggctgttg atgccaatgt cctgatttat gagcggatta
     4381 gagaggagtt gcgtaatgga ttgtctcctc aagctgctat tcatgcagga tatgagcgag
     4441 cattttcaac tatattggat gccaacgtga ctactttaat tgtagctatc attctattcg
     4501 cggtgggcac aggaccggtg cgcgggtttg cggttgtcct ttctctaggt ttgttaactt
     4561 ctatgttaac tggtattact tacactaggg ctattgtgaa ttggtattat ggtgggcgta
     4621 atgtcaaaaa attatctata ggcatataag gcgatactca gatggaattt tttaatccaa
     4681 attcaaaaat agattttatg ggcgcgcgcc gttggactgc attgttttct atattgattt
     4741 ttgtagtgtc tattggcgca ttgatgatta atggtttgaa atggggatta gattttactg
     4801 gtggaacgca aattgaaatc agttactcca aagcggctga tttaccttta attagagaaa
     4861 atctgaataa agcgggtttt aaagaagctc aagtggttag ttatggtaca tcaaaagatg
     4921 tacttatcag cattgcaccg agagcggata aacagcagag catgctggta gatcaggtga
     4981 tggaagtact gcctggagca gtaaaacaac gagttgattt tgttggtccg caagttggac
     5041 aagaattagc aacgaaaggg gctcttgcga ttattgtgtc cttgttagcc acgatgatct
     5101 atatcgctat gcggtttgaa tataaactcg ctgtaagttc agcagtggct ttgattcatg
     5161 atccggtatt gattctgggc gtttttgctt tgttcggaat tgaatttgat ctgaaggccc
     5221 ttgctgggtt gttggccgtt attggttatt ccttgaatga taccattgtt gtctttgatc
     5281 gtgttcgaga aaattttgta aaaattcgcc gaacaagtcc aatagatatt atgaatattt
     5341 caatcaatca aactctctca agaacgatca tgacatctgt gttgacgctg tttgttgtgg
     5401 ttgcgctttt tgtctatggg ggtgaggcga tatatgggtt ttcattggca ttgattatag
     5461 gaatagtgat aggtacttac tcttcaattt atgttgctgg cgctttagca gtagccatgg
     5521 gtttggataa caaggatttt ctacccagtc atcgcaggga ggtggatgat aggccctgag
     5581 gtcgaattat tatccgcgta gtatccgctt cctgacagtc gcggctcggc tttgcccaat
     5641 tcgacgaaat tcaaagtcag ccataatcgg agccgcgacc atcagggagc ggataataaa
     5701 caattacaaa attttgttaa aatgtatcct cttaaaaaga gtcaataact tttaatcaac
     5761 cagtaactta tctatttttg ctgcgcaaat aaaatcatta tgagttaaac cattaagtgc
     5821 atgcgtcata aagtgaacac ggcaataatt gtagccgact tctaaatcag ggtggtgatt
     5881 ttcaatattt gctatccagg caatcgcatt gacaaaagcc atcgtttcat aaaaattttt
     5941 aaatgaaaaa gcacgcttga taattcggtt gtcctctgtt acttcccatt ttgtgtttaa
     6001 ttgaggtagt aaatttttga tttgctcaga gttgagcgca gcgccaattc cctcgcaaga
     6061 ttcacaatgc ttactactta aatcactagt cattttgatt ccttaatttt atttttaatt
     6121 taaaaagagt tacaaataaa aatttttcag tgctgttaga aatctggaat tttgttcttt
     6181 ggtcccaata gtaaccctga tgaaattatt catcttataa gcatgcagag gccttactat
     6241 tatgccatta tccaatagat aattataaag agccatacta tcctcttcgc agtcaaaagt
     6301 aaggaaatta caggaagagg gtaagtattt tatgtttaat tcattaaagc ccgcctgtag
     6361 ctgcaacatc ccttcattat tcattttgag gctagactgg atgaagtcat cgtcatcgat
     6421 ggcagcatag gctgctgcaa gagcaacttg attgactata aatggcaatt gtactcgttt
     6481 taaaatatta ataatggatg ggttggcaat agcataacct aaacgcaaac ctgccatacc
     6541 atagattttt gaaaacgtac gagtaacaac caaattagga tgttcttcaa gccaatccaa
     6601 actattgaca gttaattggc tagcagcaaa ttcataatag gcttcatcta aaactaacaa
     6661 agtacttttc ggaatttgtt ccaataaata tttaatttct tcttgttgga ttagcacccc
     6721 agttggatta tttggattag ctataaaaat aattcctgtt tgttgattgc aagcattaac
     6781 gatatcagtg atattaacct cccagttgtg gccaataggc acactattta ttggtatttg
     6841 cagggaattg gcttggatgg cataagtgct aaaagcgtaa tcatgagtaa ggatatgtct
     6901 gtcggtatga agagcgaaac aatttaataa aatattaaac aggtaatcag acccattgct
     6961 tagaaacaat tgctcaggtt ttacttttaa gtaacttgca agtttggaca ttaaaggatg
     7021 gttccagggc gatgggtagg ttgctatgta atgcgatgac atagtctgaa tgacggaaag
     7081 ggctaatggg ctgcatccca atgggttttc attactagcc agctttataa tgtcagttat
     7141 tcccttttct tttgccaatt cttcgatgga tttacctggt acataaggaa ttaaagaacg
     7201 tattccggca tggggtaatt gttgaaaatc aatagacata actctattcc tagacagaaa
     7261 aatgttttta tattacctaa gcatatgcca gctgtcacga atgaatttat tttttagaat
     7321 ttataaatcg ttatattgaa tttatataag cgaacagtta accttcatgg gcagtccaac
     7381 ctcaggaaga gatttattat gagcaagcat cagggtttca ctctaatgga agtacttgtg
     7441 tcattaatgc taatgacaac ggtagttctg atgttagtgg aacaacagtt gaaaaccaga
     7501 gatataatca atcatcttat ttggcgaatt caaggttccc aatatctaga tcaagtagat
     7561 gaagcattat atattagtga gaatagactc cctctgccgc cttcaaatta tcattttgat
     7621 atcaggagat ctcaaaagaa aatcattttg aatgttacgt ggtttaaaaa aagccagtca
     7681 attattcgct cctatgatct ggatgctcat tgaaatggga agacaaacag gatttagttt
     7741 atctgagatg ctaattagtc tttttctggc aagtattata acgataaccc ttactcagtt
     7801 ttatataaga agcaagtctc aatacctaaa aacacaagaa cagttagaaa ttaattttga
     7861 tttgctgtgg attggtgatc tactaagcga cagtattaga aaggctggat ttactccatg
     7921 cgtaagtgtc gaaaaattaa aaacttttga ttctcgacat gcagagagga aaataattaa
     7981 ttttcaaatt gaaaatatgc cacaagttca acttcgtatt aacagaatga gtgatgttta
     8041 taccgaagtg gcgaatttta tgagcccaac acaaatagtg attgaagaat cagtaatatc
     8101 aaatataaaa cacccattaa ttatttctga ttgccggcaa ggagaagtac atacggtttc
     8161 aaaaatagat aaactaagta atggatatca aattacttta aaaaaaccaa tgcagtttcg
     8221 atacgacgaa attacttact tgggagagtg gcttgaagaa acatggttta ctaaacaaaa
     8281 tgaaaagggt gaaaagacat tacattacaa actgtttcaa acagaagaat taagtccatt
     8341 cattcatgaa ttttatgtta ccagacgtct tattaaacag aggtcagtat tggatttttc
     8401 aattggtagc gataaggaaa cgctacgcaa aattagtgtt tcggtacgaa atttatgaaa
     8461 aaaaccgatt ccggtttcat tcttttaatg acgctctgta taactttctt gatgagctta
     8521 ttggttttgg caagcctcca gcagatattc ttgcaattca aagcactcaa ttcacaggag
     8581 acatttcatc aaaatttcta tcagttggaa cattggatga tgcaactcgc tcgttcaccg
     8641 ttattgtatg caggtatgga ttgttatctg caaaaggatt atggagctaa taaaatagca
     8701 aacgagttag ttaacaataa aggatgtgtg ctcatattaa atcagaaaaa atacagatat
     8761 tttattgagg acctgaatga attttcttgt ttggttctta ataaggatgg gcaaaaatat
     8821 gcttctcacc attttagatt caccgtatta caagacgagg agcatggcga atctactgtc
     8881 atgcaattaa ggtttatcaa attgggtagc aatttgtcct cttgtccaga gggggaaata
     8941 caggtgaagg aaggggtaag cagctggaga tacttgccag attataaaaa ttttgaaata
     9001 ttaactggtt gaaattattg aaaaaaagct attataaccg gatgtttttt gtagtaagat
     9061 ggactaataa caataatttt tagttgcatt tttctccatc acgtgtaaaa attgacctcc
     9121 tatgaaatcg aaattaattt atctgacagt agccttggct gcaattatct taagcgcagc
     9181 agtgtggtgg ttgcaatctc ccaaaccaac tttcaggcag gtaagcttta agcatttacc
     9241 tgggtgggag aatgcacaac ttaaaaaatc gttagaaact tttcaaacat cttgtcgagc
     9301 ctttctaaaa caaaatcctg aacgtgttgt tggcacagag cagattgatt tacaagcaaa
     9361 agattggcat ccagcctgtc gtgctgcttt aaaaataaat cctgttgatg aacaatcagc
     9421 aaaagtattt tttgagaagt ggtttgcccc tgtggaattc tatgatgata aaccagtgaa
     9481 aggtttattt acgggctatt atatgccttt gattaaaggc agttatacca aatcaaagga
     9541 atttagtgtt cctatttatg aattacctag taatctggta actgttgatt tgggtttatt
     9601 tcttcctaat ttaaaaaaca agaaaatcat tggacgaatt gcaggtaata aagtcgtacc
     9661 attttacact cgagagcaaa ttaataaagg ggcaattaaa gacacagctc gtgttttggt
     9721 ctggatcaat agtcctgttg acaggttatt tttagaaatt cagggctcag gaatcattga
     9781 gcttgaggat ggtaaacgaa tttttatagg ctatgatggc caaaatggtg ctccttatac
     9841 tgctattgct ggtgtactga taaaaaaagg tattatgacc aagcacaatg cctccatgca
     9901 aaggattaaa aagtatctgg aagctcatcc caaacaaatg gataaaataa tcaatcaaaa
     9961 taaatcattt gtttttttcc gcaagttgtc attagaagcg gctttaggtt cacagggtgt
    10021 ggcattaaca cccggttatt ctcttgctat tgataaacaa tggataccaa tgggagcgcc
    10081 tctgtggtta aacactacaa gaccagatag taaaaatcct gacgtcaata aacctatgca
    10141 acgtttgatg atagctcaag acactggtgg ggctattcgt ggtaaagtaa gaggtgatgt
    10201 attttggggt ggtggtgaca aagctacttt gattgctggc catatgaaga acgagggtca
    10261 ttattggctt cttctgccgg cacatgcatt atcgcgcttg gcacagttga agtaggggcg
    10321 taagtcctgt ctttgcaatt ttttatttgg ctattgggat tcaccagtcc tgtgacaatg
    10381 aatcccggat agcattgtaa atcatttgtt tcaagaaata actctatgtt tttaagattg
    10441 aggctcatta atttctttct tttcggtttc cgatttcaaa gcttcagttg gtgatgaatc
    10501 attaactgga gtagctgtat tcccttttag agcctcttgc agagtaatga atttataacc
    10561 gttgcttcta tacatttcaa tgatatccgc caaacacaaa ctattaatca gattcgcatg
    10621 gatgagtaaa atatgtttaa caggttgtcc ttctactttc ttgacttttt tttctgctct
    10681 cagggtctgt tgccagataa aagcaagata gcgtttttta aattgagcta aacgttgtgg
    10741 tctttgtcta tatggtattc tataaaattg cgcattgaat tcataatctt tgctatctat
    10801 agtgacagga gcaatcgtgt attgatgggc agccagccac tcatgcactt tttgcttctt
    10861 ttctccactg ccttcagcaa gataaggata acggaaatat tttggctcag tcataacagg
    10921 ggatagaacg gtatccgctt tttcgatatc agcaatataa ttttctgcag tcattgaatt
    10981 taaacttcga tgcttatagg tatgattgcc aattgaaaaa ccttgattac gaaaaatttc
    11041 cagaagctcc cattcatttt tcgctatagc ccctcctatg gcaaaacctg tggcaggaac
    11101 ctgattgtca accaaagtgt tcactatggc cataaatctt tcctgggttc gcttcagatt
    11161 tcctggagta tttgttccag agcccacaaa aggtaaatcg tcaatagtaa tggcaatttc
    11221 atgatcttca gcgaagcatt ggtggttcaa tattaaaaat aatgatgcta caccgagtag
    11281 acgttttaac atattttccc ctcttttcaa tttggaaaat ttcttattat tttaggcatt
    11341 taatttaact atagacgata aattaaattt ccacctgttt gaaaatatgt atgtattttg
    11401 agagggttta ggtacaataa atacttcgat ttttcggcta ttggataatt tattaaagtg
    11461 acaaatagag aattacttaa attaatttga gttatgcata agggttatat aaggagcact
    11521 cgatatcccg ggtccgtttg gggcagggag atgtgtaagc atcctctcaa agcctaagca
    11581 tgaaatatta cattccatac taggcttcga gatggcataa atgtctcctc agcgtgagcg
    11641 attcaagata acggctgatc ttatccataa ctcacatgaa attagggctg atcatgtcaa
    11701 acaataagac aaggccgtgg atataatctg agaaattgaa aggatactgc cttacccgga
    11761 attttgttaa acaggagttg ataatgacaa aagaaaaatc tgaatcagga aattcaccat
    11821 caaattcaga agaacatggt actttaggcc cggatgttta tgagaatgca agaaagctaa
    11881 atattcaatt ggcaacaaga gggcctgcca tactgtatgc attaggtaaa gccagtgagc
    11941 aagtaactaa tggaaatgtt gtaacggcgg ttggtggcat ggttgttggg gcggtaggtt
    12001 tttttggtaa tcgtttttat cgaaacaagg gtgatgattc cgcgtatact tatatgggac
    12061 aaattgttgg tgcggtgcaa tttactgccg gattcacact gttggcatta caggcatttg
    12121 aggtgtttac agatactgga gaaacaagcc ccttgctcaa tgtggtaaaa ctaagtgggg
    12181 tggcgttaac gttaaccgaa agcgctttaa ttttaatcaa ctcctattta tatggggatg
    12241 gatcagccaa accaacatca ataacaagcc ctaagattga ttgaatataa taaaaaggct
    12301 aacagggttg agatataaag cagcttaaat caattacttt gattttaaaa taaaggacta
    12361 tttatgactt attctttttc caaaccagtg tcttgggtgt ttttgtttac cgcagtcatt
    12421 tatttggtca gtctttcgtt tatccaatat cctgccacta ctgttttaaa acctattcca
    12481 atagtttgtt taattgttgg ggtattcaga acatctctct cctcatcggc taaaattctc
    12541 ctgatcttgg cacttgtatt ttccttggca ggggatgtgg tattgacttt acccttttca
    12601 ttgcagttgg aattaggtat cgcttgcttc ttgttagctc attgttttta tatcactttg
    12661 tttttaaaat catttgaatt caataggtta catttatttt actatcttcc tattttttta
    12721 ttcatggggt ttgccgcatt taccatgatt ccatatcttg gcaatttatt aattccagta
    12781 atgatttatt tctgtgtctt gatgcttatg gtatttagtg cttttcaggt aaaaaaggaa
    12841 actttaacga ttagcagtgg agccttgttc tttttgattt ctgatttaac tttggcactt
    12901 aacttgttta tttatactca ggctgatgta aggatttttg ttatgtttac ctattacgta
    12961 gctcaattct tattaacttt tggtctggtc agattatatg aaaaaggggg ttgatgaatt
    13021 tattattcag ataacataaa aagtgttcat caagttaaat acttttttac tactcttgta
    13081 gtctttatta aaagaattac ttatgaatta tataaaccaa tttgctaaat aaaataattg
    13141 ctttaaagaa tcaaattgaa aa
//
LOCUS       DACTJE010000085        13093 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.085, whole genome shotgun sequence.
ACCESSION   DACTJE010000085 DACTJE010000000
VERSION     DACTJE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13093
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.085"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..332
                     /gene="pieF"
                     /locus_tag="I9760_001835"
     CDS             <1..332
                     /gene="pieF"
                     /locus_tag="I9760_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PieF"
                     /protein_id="HAT5043601.1"
                     /translation="QAISSGGIVEKYTPIFSLTKESDNELTLELSGVARGYYIIPSEL
                     TSSQARAAHLITLLTRAEESQTTDMHKILNSFVSGKITSGSMFNFENDGSFKREPEEA
                     YNLINKI"
     gene            556..975
                     /locus_tag="I9760_001836"
     CDS             556..975
                     /locus_tag="I9760_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAT5043602.1"
                     /translation="MKALYTTIAKAHGGRNGHVETTDGLLKLDLAMPRELGGEGGATN
                     PEQLFAAGYAACFESAIRHVANVQKISLEDVSMTSEVSLYATPEKGFKLGVALHAHIT
                     GLNQNEAEALVAKAHEVCPYSNAIRGNVDVKLSVSVK"
     gene            1009..1632
                     /locus_tag="I9760_001837"
     CDS             1009..1632
                     /locus_tag="I9760_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAT5043603.1"
                     /translation="MYTLYSFGTPNGFKPTIMLEELTQPYTIELIDITQGEQFNPDFL
                     KISPNNKIPVLHDQTNDFYLMESVAILQYLAEKHRQFLPETTKEKYTIIQWCYFQAAH
                     IGPMFGQFGHFHRYAQERVPYAMTRYADESMRLLGVMEKQLVKAPFIGGSNYTIADMA
                     IWPWIWCFQFIYGQTIDAKQFPSLMSWYQRISERQAVRATLTAYGRL"
     gene            1976..3883
                     /gene="pieE"
                     /locus_tag="I9760_001838"
     CDS             1976..3883
                     /gene="pieE"
                     /locus_tag="I9760_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PieE"
                     /protein_id="HAT5043604.1"
                     /translation="MESVFSGESKFLLHHVSIHMNISTYEFVNNTITEESRVMALSEI
                     IINDIVQSQTNAVISESNYHILRQYLLERKPLSRVLNPINSLLSEHCNQDKQAIINHL
                     TQLACESQRNHDLQEARSDEQEGINENSLMINYRSELLTLENTLKELDTKCYQQQRQY
                     NQVSRQFNELKVNMTHINQSIDRVRYERQLLDQQLNHPYSDGNVYTNPPTHTGIYPDL
                     SPPQDRHTRDRLLQEENRLIEERQKLMHLINSKETEKNKEEQNLNRLHKEKKEAEGRY
                     SEVKHQIDVVLPNSEQQRQIRNQERLARENARASYDPHLLQLSHKNLEALKSQIETQI
                     RELDERRNQLMGEATDISYKIYLTQLEQVLQESEKGPQITFNENNALKMILAMMKNME
                     EMAEKEKDISSSIDKERHNLHSLQKSLVGYTRQLERYLTSDPHLVKQNKALTEENTQL
                     KQYSESADNWRTKAFYASLFSVGGSLISTGILNTFIISPVFFAIPGALAALGVVSLVI
                     AVVSYCQKYFSDMQMEQNNQKIEKNELILMKQWKKANELSLSTIPSLNAKIEQSEKEL
                     YALEQKLQDQQHAMSLLLNKAQNVTSNYGGSSNFFGNTTGNVFYLPSAPLHEDSPLYP
                     EIEGERVNYGY"
     gene            complement(4435..5184)
                     /locus_tag="I9760_001839"
     CDS             complement(4435..5184)
                     /locus_tag="I9760_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043605.1"
                     /translation="MSKVKAKIIAFSTVMLFLTSINNYAKGETPMNQYHQTNEATMIK
                     MKSKLSNSSEFEKFLESGGRLVKQTEPNTKVWFALKGNDNSLIIFDAFYDSKGREAHF
                     AGQVATALKNNSEKLVLGSWENGVLKNIVNSKVLASKLPEKSVPVTVANYIEFTANDG
                     KSEQLAALLINAAEIVDKTEPQTAYWFTLQINDNTFAIFDAFSDESGQKAHFSGKVAL
                     SLKNHSGLLIKNGWEQGVLPNIQNLKVITNI"
     gene            complement(5242..5808)
                     /locus_tag="I9760_001840"
     CDS             complement(5242..5808)
                     /locus_tag="I9760_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT5043606.1"
                     /translation="MPMNISNTKERILAVAEALIQKDGYNAFSFKDIATAINIKTASI
                     HYHFPSKEDLGVAVISWHTDKIAAVLSDISNNSSLSAKEKIQKFFDAILTLTYNSENK
                     MCLGGMFASDFQSLPVSIQNQAKKFFELIIEWLKGVLETNGYDNESSLSLAKQIISLV
                     EGGLLLARLYGDETFLEGVRHFIDQTIK"
     gene            6053..6280
                     /locus_tag="I9760_001841"
     CDS             6053..6280
                     /locus_tag="I9760_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043607.1"
                     /translation="MFFKRSNPHVTPQDLQKVIQNLNAQRELTERQLKEGSISQKTGQ
                     EEMQRLSSLIGAYQNNLMAALDDQQNTNCLK"
     gene            6617..8182
                     /gene="pieD"
                     /locus_tag="I9760_001842"
     CDS             6617..8182
                     /gene="pieD"
                     /locus_tag="I9760_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PieD/LirF"
                     /protein_id="HAT5043608.1"
                     /translation="MIKDILNTIASLVEENVVVFASERGKQLLQKVKADLVSFKPINF
                     YALDAGKQITDALKLIESYCIEMNRIFDELNHQSSVSLANEKLTHKIKNILTNLKNKQ
                     NKITQLREKIPEGMLDIKRALYIVENSFLSESEPEEKELRSKFKRIKKFMSNSNKEYL
                     DLIKKGGLLNTVLLDTTTLYWNQLHEYSTLLNQCRITLQKSHHLTKEGQLALRCFNSA
                     IQKMPPKKHIINALKILNKPNSKTEAIEKIGFVSPHGREACMQLTCWGKKFFYIPRSE
                     EMYHFDYDPKEDLNETGGNCFGESMMFIHFLSKGAIRWLCPEAGLINFQLDQTRNLKF
                     KKATLGEGETLVSDNSPHDSLEWEDMRPVLLDNPYFKPGSLCGVIFSMNDYTKAKREF
                     TAGHMIVVAKLDTNLNPYKYIIYEKDFGAFGLTDDESLEYLISEQILPLYRGMNYSKI
                     KLVKYGEASTETYDLLNKIKPQTGIPQKESTASDSINTYFDNAFFKLSKETNKENVID
                     QVNQTDVFGFCRN"
     gene            8587..11553
                     /gene="pieC"
                     /locus_tag="I9760_001843"
     CDS             8587..11553
                     /gene="pieC"
                     /locus_tag="I9760_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PieC/LirE"
                     /protein_id="HAT5043609.1"
                     /translation="MLKFYSDSGAKMIKKLLIKVEKLINNLPKEFKLSETVLFSIKED
                     INSLASRFDGSKGYINYYSPKVVQQINDKQKLLEKYKSYLTALLAVLICSKQSKDIRD
                     NKKLQPVYEITDLKNLLERKDVEKIINEITQFDEDQRELQRIERQIQKDEEKEDNQKM
                     HLGKRSKLELRRAELSKRVQLFNNKLKNIPQAEEIKAVLDYCQKYIEKHINLSKQLPK
                     GMLDVKRALSLFDEINSTKTQIRKLSKDKLDSSGQIKLEGLKKELTKAELALQFSYSE
                     EEDKTLKDMLNDLKRLMKMSIRDYQKLATSGGEINVFLHKTTGFYWESLFLYRNVLCE
                     INEYLKKNKKKLSEQGMLVAECISKAFSQMPELDTIEKALEKVKRIDGDLHRQEEYND
                     KINGAWIRFYKKGPVKFVQKVLEQNLDEKIFYIPYNPHNYVLDHPAQSDFADTLGNCY
                     GETQMFLKQINSKSPSINNICPERVLINYQLDQSRTIAKNTKKEKIGIFEADEKTKAQ
                     VTWDGIKDILTSKVTSTAFGDVCMITLTGAQVSGREKLVGHGIGLIKMKNPAPYKYVV
                     YDYAFGGAMGFSSDTQLELFFKQILEGEGSYVPFKECLVEKVGEVSKECQQFINGNPG
                     IASLAQKSLDKTCKRDFWDKDRISFLIRYHPIGTDESRILDLIEKLPLSKDRIELYEQ
                     LVKEPKIGIGNLVKCIIRSGKISYLANLLTTNLLTVDITNKIKFKLNEQVKKEIVALI
                     NNDQIEPNTTCKLFSNNQAIVLATYKKDKNSLQYADIDPVVTLLQGKNTIVPSDACRA
                     FPKAEKIVIAAYKSDPLSLQFADLGLVQQLLRKGSINLEHASKAFTESSQFKEANEAY
                     QLLKSLKLLPKNEKETEKAKRIELLKSIDQKLEITNLRQELYPLISNMCIRLLDDIRI
                     LSLEDTIIKNTRLSKLISSKQCDINTLKTLENKKMELTKTCSLAKSNFTLFGATDNKV
                     DGGNANTHNHITPENK"
     gene            complement(11817..>13093)
                     /gene="pieB"
                     /locus_tag="I9760_001844"
     CDS             complement(11817..>13093)
                     /gene="pieB"
                     /locus_tag="I9760_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PieB/LirD"
                     /protein_id="HAT5043610.1"
                     /translation="KEKILINLDKFHTKEDGSFRFEPKNIDTSLPILSFQKEDATKFP
                     LFNDLNIKINKSDSIDFKAAYISETISSLIPEYTKENTYRLTSLTYELPLNPSLALQI
                     IAVFDNYLTQTKEARFSKKSIKQSIDEHGNLKICLMEFFDDSHYIAMWDEEMGINPLE
                     IRKRKFSDKHPFLQGFINASNIPEIYLPVIDNLKKYYAFCEIEHLTISECLNNTKLNQ
                     LISTNKNPYLLLTDLSPSQQLSIVNEIKRDLLKGNSSIPWEKIINRIFEYKIDNLFRP
                     SIGQKAHDGGKYNLNYTEIEFLKHIANNLTLKGNKICISNLNENPAYLKYYAFGCKNN
                     NIFEKELRFLGKYVGLEFEDCDFHKEIVLTQKCSDYLKEKGFTYLIQLKNLMNNLQQP
                     LTKNIRLMLSDVYLNNETNNNINENDLSYTFT"
BASE COUNT         4504 a         2107 c         2342 g         4140 t
ORIGIN      
        1 cacaagcgat ttcttctggt ggtatagttg agaaatatac acccattttc agtcttacaa
       61 aagaatccga caatgagctc acattagaat tgtctggagt tgctagagga tattatatca
      121 ttccttcgga gctaacctct tctcaggcaa gagcagcgca tcttataaca cttcttactc
      181 gggcagaaga atctcaaact acggatatgc ataaaatact taactcattt gtttcgggaa
      241 aaatcacgtc aggcagcatg tttaattttg aaaacgacgg ttcatttaag agagaacctg
      301 aggaagcata taacttaata aacaaaattt gataagctat ccaggccaag gatggctcta
      361 tattttctga ttattgagac taattttctt agtttgtaac taatttaagt ttgtattgtc
      421 ttttccttgc ttatcaagac tgttttcttt ccaggcgcga attaaagtgc ggcatgaaat
      481 ttcttccttt ccgtttgaca aacagcaaaa tactaaataa ttttaattcc ttaccatttt
      541 aaggaatata aaattatgaa agcattgtat acaacaattg ccaaggccca tggtggacga
      601 aacggccatg tagaaactac agatgggcta ttaaaacttg atttggcaat gccgagagag
      661 ctaggagggg agggtggtgc cactaatcca gagcaattat ttgccgcggg atatgctgct
      721 tgttttgaga gtgctattcg tcatgttgcc aatgtgcaaa aaatttcttt agaggatgtt
      781 tccatgactt ccgaggtctc tttgtatgct actccagaga agggttttaa acttggagtt
      841 gcgttacacg cccacataac aggcttaaat cagaatgagg ccgaagcatt agttgccaaa
      901 gcgcatgagg tttgtcctta ttccaatgca atacgtggta atgttgatgt gaaattgagt
      961 gttagtgtca agtaataaat aacttccatc aatcaataag gatatcttat gtatacgctt
     1021 tattcatttg ggacgcccaa tggatttaaa cccactatta tgttggaaga gttaacgcag
     1081 ccttacacca tagaactgat tgatattact cagggtgaac aatttaatcc tgatttttta
     1141 aaaatttcac caaacaataa gatacccgtt ttacatgatc aaaccaatga tttttaccta
     1201 atggaaagtg ttgctatttt gcaatattta gctgaaaaac atagacagtt tttgcctgaa
     1261 acgacaaagg agaaatatac tattatccaa tggtgttatt tccaagcagc acacataggc
     1321 cctatgttcg ggcagtttgg tcattttcat cgttatgctc aagagcgagt accctatgcc
     1381 atgacgcgat atgctgatga atcaatgcgt cttctaggtg ttatggaaaa acaactggtc
     1441 aaagcgccat tcattggtgg atcaaattat actattgctg atatggctat ctggccctgg
     1501 atttggtgtt ttcaattcat atatggacag actattgatg caaaacagtt tcccagttta
     1561 atgtcatggt atcaacgaat tagtgaaagg caggctgtta gagcaactct aacggcatat
     1621 ggtcgattat gatcacgaat ctccattaga agtaagcgag caaagttgaa cttatctgat
     1681 aattaatggc tcccaattga ggtaatggtg tcaagtgggg tgttttacct ggttattctt
     1741 tcaatagatt gtctcatctg aaaaattgag gttgtgcagc tgccaatcaa gtaatctcta
     1801 ttgtctttat atcctattta aaatttagtt tactctctat atctgtaatt ttgttcagtc
     1861 ttaacaattc cttaacaatc tcttaacaat tccttaacat ttgtctgcca taatgccctc
     1921 gatttaaaca taaaaaacac agggggtatc acactgtttt gaatagaact aggaaatgga
     1981 gtctgtattt tcaggggaaa gtaaatttct tctgcatcat gtatcgattc atatgaatat
     2041 ttcaacatac gagttcgtta ataatacaat tactgaggag tcacgagtca tggcgttatc
     2101 agaaatcatt atcaatgata tagttcaatc tcaaactaat gcagtcatta gcgaatcaaa
     2161 ttaccatatt cttcgccaat atctcctgga gagaaagcct ttatcaagag tactcaatcc
     2221 aattaattct ctgctttctg agcattgcaa tcaagataag caagcaataa taaatcactt
     2281 aactcaactg gcttgtgagt ctcaaagaaa tcatgactta caagaagcaa gaagtgatga
     2341 acaagaggga atcaatgaaa attctttgat gattaattac aggagcgagc tgcttacttt
     2401 ggaaaataca ttgaaggaac tggatacaaa atgctatcaa caacaacgtc aatataatca
     2461 ggtgagcagg caatttaatg aacttaaagt caatatgacc cacattaatc aaagcattga
     2521 tagagttcgt tatgaacgtc aattgcttga ccagcagcta aaccaccctt actctgatgg
     2581 taatgtttat actaatcctc ctactcacac aggtatttat cctgatcttt caccacctca
     2641 ggatcggcat actcgtgata gactgttgca agaagaaaac agattaattg aggagcgtca
     2701 aaagctaatg catctcatta attcaaaaga aaccgaaaaa aataaagaag agcaaaattt
     2761 aaataggctt cataaggaga aaaaagaagc tgaaggacgt tatagtgaag taaaacatca
     2821 aatcgatgtt gttttgccaa atagtgaaca acagcgtcaa atcagaaatc aagaacgtct
     2881 cgctcgagaa aatgccagag caagctatga tccacattta ttgcaacttt ctcataagaa
     2941 tcttgaagca ctgaaatctc agattgaaac tcagattcgg gagctggatg agagacgaaa
     3001 tcagttaatg ggcgaagcga cagacataag ttataaaatc tatttaactc aattggagca
     3061 ggttcttcaa gaatcggaaa aagggcctca aattacgttc aatgagaata atgcattaaa
     3121 gatgatttta gccatgatga aaaacatgga ggaaatggcg gaaaaagaga aagacatttc
     3181 tagctcaatc gataaagagc gacataatct ccattcattg caaaaaagcc ttgtaggata
     3241 caccaggcaa ctggaacggt atctgacgtc agatccccat ttggttaagc aaaacaaggc
     3301 tcttaccgaa gaaaatacac agttaaagca atatagtgaa tcagcagaca attggagaac
     3361 gaaagcattc tatgcgtctc ttttcagtgt tgggggtagt ttaataagta ccggaatatt
     3421 gaatacattc atcattagtc ctgtattttt tgcgattccc ggtgctcttg ctgcgcttgg
     3481 tgtggtttcc cttgttattg cggtagtctc ttattgccag aaatacttta gtgatatgca
     3541 aatggaacaa aacaatcaaa agattgagaa aaatgaatta atactaatga agcaatggaa
     3601 aaaggctaat gaattaagtt tatctacaat tccaagcctg aacgctaaaa tagaacaatc
     3661 agagaaagag ctgtatgcgc ttgagcaaaa attacaggat cagcagcacg caatgagcct
     3721 gctgttgaat aaagcgcaaa atgttaccag caattatgga ggaagcagca atttctttgg
     3781 taacacgact ggtaatgtgt tctatttacc ttctgcccct ctgcatgagg actctccact
     3841 gtatccagag attgaaggtg aaagagtaaa ttacggctac taataagcta aaatgactgg
     3901 tctttaattt ggtttcaatt tgaaggccag tatctatgat tattcaaatt ctgaattgct
     3961 ctttacaatc tgctttgtaa aaatattttg ctcatacagg atttaaaatg aagtattttt
     4021 tagttaatgc tgtttggttt atgagggctg gggctgattt aaaggcagta tttttattgt
     4081 agcctcatta atgtgctaaa attgtacaca tgggtaacct gatatcattg cttgaatgct
     4141 aaattacatt ctcttattga aaaggatttc catatgaaaa cagtcctaat atttattatt
     4201 tttttatgtt cttttcaaat acaggcggca tgcaaatgca actgtgaccc gacagacaat
     4261 aagatgtgtg ccagtcagaa tgatatggag catccttgcc cttcagtttg tgcgtcggca
     4321 gcacctggta tggcacctat gtttacagca tgtcctgtat caaaggttac tgatccagtc
     4381 acaggagtta ccaggtgggt caatatgtgt aactagcaat aaatacctga ttgatcatat
     4441 gttggttatc actttcaggt tttgaatgtt aggtaaaaca ccttgttccc atccattttt
     4501 aatcaagagt cctgagtgat tttttaaaga cagtgcaact tttcctgaaa aatgagcttt
     4561 ttgaccagac tcatcagaga aagcatcaaa aattgcaaag gtattgtcat taatctgcaa
     4621 agtaaaccag taggctgttt gcggctcagt tttatcaaca atctcagcgg cattaattaa
     4681 taaagcggca agttgctctg attttccatc gttggcagtg aattcaatgt aattggcaac
     4741 agtgactgga actgattttt caggtaattt agaggcgagc actttactgt ttacaatatt
     4801 ttttaatacg ccattttccc agcttccaag aactaatttt tcagaattgt ttttaagagc
     4861 agtagcaact tgaccagcaa aatgggcttc tcttcctttc gagtcataaa acgcatcaaa
     4921 gataatcaag gaattatcat ttcccttaag tgcaaaccat acttttgtat taggctcagt
     4981 ttgtttcaca agcctaccgc cagattccaa aaatttttca aattctgagg agtttgataa
     5041 tttgcttttc atttttatca tagtcgcttc atttgtctga tgatattgat tcataggtgt
     5101 ttctcctttt gcatagttat ttatcgatgt taaaaacaac attactgttg aaaaagctat
     5161 aatttttgcc ttgactttac tcatataaaa tttccccata aaatctacct actgatagat
     5221 agtttgtggt gttaaaaaaa atcactttat tgtttgatca ataaaatgcc gaactccttc
     5281 caggaacgtt tcatctccat ataatcttgc taataataat ccgccttcaa ccaatgaaat
     5341 aatttgtttt gcaagagata atgaggattc gttatcatat ccatttgttt caagaactcc
     5401 tttaagccat tcgatgataa gctcaaaaaa tttttttgcc tgattttgaa ttgaaacggg
     5461 taatgattga aaatcagagg cgaacatacc gccgaggcac attttgtttt cagagttgta
     5521 ggtgagtgtt aaaatggcat caaagaattt ttgaattttt tccttggctg ataacgacga
     5581 attattgctt atgtcagata gcacagcagc aattttatcg gtatgccagg atataacggc
     5641 aacaccaaga tcttcttttg aaggaaaatg ataatggatg ctggcagttt taatattgat
     5701 ggctgttgca atatctttga aactaaaggc gttatatccg tctttttgga tcaatgcttc
     5761 cgcaactgct aatatccgtt ctttcgtatt acttatattc ataggcataa attctaccta
     5821 ttcatagata gattgtcaag ctttttatag ttttctatat caaattaaaa taacacttgt
     5881 ttatctaaat agaagtaggt ggtggactgc tcattcatta attgattctg atctattgga
     5941 tcaatatctt tatgcatcgc tatgtcaatt ggccttaaag actttatgtc tttattgatc
     6001 tcgaaaaaaa cgggaggtaa tatatgtaca tcagttcata atagaggtca ttatgttttt
     6061 taaaagatca aaccctcacg taacaccaca agatttacaa aaagtgattc aaaatttaaa
     6121 tgcgcaacgt gaattaacag agcgtcaatt gaaagagggc tctatatcac aaaaaacagg
     6181 gcaagaggag atgcagcgtc tatcaagctt gattggagca tatcagaata atctgatggc
     6241 agcattagac gatcaacaaa acactaattg ccttaagtag ctaatctttt tgaggtataa
     6301 aagggcagtc attttgatta atggcataat ccactcataa tctgaatggt catttacgtg
     6361 ttgacaggat tttgagtacg ctgtgtcatt ctatgtacca agaaaacagg gatgttattt
     6421 cctgccctat caatgtatct gaaggcttcc ccagaccttt actttaaatg atgttcatga
     6481 gtctatttaa gagattggaa ataaaataga ttattgaaat ttatgaataa ttaacccaga
     6541 aagtacttgc tttattcaat ctgtacaggt atttctattc aatcaaagcc cattctattg
     6601 agtaagagag tattaaatga taaaggacat tctcaatacc atagccagcc tcgttgagga
     6661 aaacgtagtg gtatttgcaa gtgaacgtgg aaaacaactt ttacagaaag ttaaagccga
     6721 tcttgtatcg tttaaaccaa ttaattttta tgcattggat gcgggaaaac aaataacaga
     6781 cgcgcttaaa ctaatagaga gctattgcat tgaaatgaac agaatatttg atgaattaaa
     6841 tcatcaatct tcagtttctt tagccaatga aaaattaact cataaaataa aaaacatttt
     6901 aaccaactta aaaaacaaac aaaataaaat aacccagtta cgagaaaaaa ttccagaggg
     6961 catgcttgat atcaagcgag cattgtatat tgtagaaaat tcttttctta gcgaatcaga
     7021 accagaggaa aaagaactga gatcaaaatt taagcgcatt aaaaaattta tgagtaattc
     7081 aaataaggaa tatcttgatt taattaaaaa gggaggatta cttaacactg ttttactgga
     7141 taccaccact ttgtattgga accaattaca cgaatactca acattattaa atcagtgtcg
     7201 tattacttta caaaaatctc atcatcttac caaagaaggg caattagcgc tccgctgttt
     7261 taatagtgcg attcagaaaa tgccaccaaa gaaacatatc attaatgcgt taaaaatatt
     7321 gaacaagcca aatagcaaga cagaagcgat tgaaaaaata ggctttgttt cacctcatgg
     7381 tagggaagcc tgtatgcaac taacttgttg ggggaaaaaa tttttttata tacctcgtag
     7441 cgaagaaatg tatcattttg attatgatcc taaagaagat ttgaatgaga ctgggggtaa
     7501 ctgttttggt gagtcaatga tgtttattca tttcttatcc aagggagcca tcagatggtt
     7561 gtgtcccgaa gcagggttaa ttaattttca gttagatcaa acaagaaatt taaagtttaa
     7621 aaaggcaacc ttaggcgaag gggaaacatt ggtatcggat aacagtcccc atgattcatt
     7681 agaatgggaa gatatgaggc ctgtgctttt ggataatcct tactttaaac caggcagtct
     7741 ttgtggtgtc atattctcaa tgaatgatta cacgaaagca aaaagagagt ttacagcagg
     7801 gcatatgata gtagttgcca agctggatac taacttgaat ccatacaaat acattatata
     7861 tgaaaaagat tttggtgcat ttggattaac tgatgatgaa tctttggaat acctaatcag
     7921 tgaacaaata ttaccactct atagagggat gaattattca aaaattaaat tggttaaata
     7981 tggtgaggct tcaactgaga cctatgatct tttaaataaa attaagcctc agacaggtat
     8041 tccacaaaaa gagtcgacag ccagtgattc tataaatacc tattttgata atgcattttt
     8101 taaactttct aaggaaacta ataaagagaa tgtaattgat caagtcaatc aaacggatgt
     8161 gtttgggttt tgcagaaact aaaccaaaat cttactgtgt gcttttaagg aactccaaaa
     8221 acctatgaaa caaagagaga gctatgctta gctccagagt caatatctat tttctaattt
     8281 acgttgatta tttgggcaaa agataaaatc gaatattttt gctcaagttt aattcaataa
     8341 ctaatgtaat taaacaattt acttgcaaaa tgttcatttt ttgatatcat tactaccgat
     8401 aatatcttct ctgagtaagt cagaggactg acatcacttg attctacagg tgtttagttt
     8461 aatcactatc attaaacggg tatatcgtga ttttctggtt ttttataaac caaggaaata
     8521 gacgatcccg taattccttg gttttgaaaa cttcattact acaaatttca gttaaatgtc
     8581 actgacttgc tgaaatttta ctcagattca ggtgcaaaaa tgattaaaaa attgttaatt
     8641 aaagtcgaaa aattaattaa taatctccca aaagagttta aattatctga aacagtttta
     8701 ttctccatta aagaagatat aaacagcttg gcaagtcgtt ttgatgggtc aaaaggctat
     8761 attaattatt attctccaaa agtcgtgcaa caaatcaatg acaaacaaaa gttacttgag
     8821 aaatacaaaa gttatttaac tgcattactc gctgttttaa tttgttctaa gcaaagcaaa
     8881 gacattagag acaataaaaa acttcaacct gtttatgaga ttaccgattt aaaaaattta
     8941 ttagaacgca aggatgtcga aaaaattatc aatgaaatta cccaatttga tgaggatcaa
     9001 agagaacttc agagaattga acgtcagata caaaaagatg aggagaaaga ggataatcaa
     9061 aaaatgcatc ttggtaaaag atccaaatta gagcttagga gggcagagct ttctaaaaga
     9121 gttcaattgt ttaataataa gttaaaaaac attccacaag ctgaggaaat caaagccgtt
     9181 ttagattatt gtcaaaaata catcgagaaa catatcaacc ttagcaagca attaccaaaa
     9241 ggaatgcttg atgttaaacg agcattgtct ttatttgacg agataaattc caccaagacc
     9301 caaattagga agttaagcaa agacaaactg gattcttcag ggcaaataaa acttgaaggt
     9361 ttaaaaaagg aacttacaaa ggctgaattg gctttgcaat ttagttattc agaggaagaa
     9421 gataaaactc tcaaagacat gctcaatgat ttgaaaaggc ttatgaaaat gagcatcaga
     9481 gattatcaaa agttagctac atctggcgga gaaataaatg tttttttgca taagacaact
     9541 ggtttttatt gggaaagcct ttttctgtac agaaatgttt tgtgcgaaat taatgaatat
     9601 cttaaaaaaa ataaaaagaa actgtctgag caaggaatgc tggtagctga atgcatttct
     9661 aaagccttca gccagatgcc tgaattggat accattgaaa aagctttaga aaaagtcaaa
     9721 agaatagatg gtgatcttca cagacaagaa gaatataacg acaaaatcaa tggtgcttgg
     9781 attcggtttt ataaaaaggg tccagtaaaa tttgttcaaa aagtccttga acaaaactta
     9841 gatgagaaaa ttttttacat accgtataat ccgcataatt acgtgctgga tcatcctgcc
     9901 caatctgatt tcgccgatac cttaggaaat tgctatggcg aaacacagat gtttctcaaa
     9961 caaataaaca gtaaatctcc ttcaattaac aatatttgtc ctgagagagt attaattaat
    10021 tatcagcttg atcaatccag gacaattgct aaaaatacca aaaaagaaaa aatagggata
    10081 tttgaggcag atgagaagac aaaagcacaa gtaacatggg atggaattaa agatatctta
    10141 acatcaaaag ttacctctac cgcttttggt gatgtgtgca tgatcacttt gacgggagct
    10201 caagtttctg gtcgtgaaaa attagtaggt catggtattg gccttatcaa aatgaagaat
    10261 cctgctcctt ataaatatgt ggtatatgat tatgcttttg gcggtgcaat gggattcagc
    10321 agtgatactc aactggagct attctttaaa caaatccttg aaggagaggg atcttatgtt
    10381 ccttttaagg aatgtctggt tgaaaaagta ggagaggtga gtaaagaatg tcaacaattt
    10441 attaatggta atccgggtat tgcttctctg gcacaaaaaa gtcttgataa aacatgtaag
    10501 agagacttct gggataaaga tcgcatcagt tttttgataa gatatcaccc catcggcaca
    10561 gatgaaagca ggatacttga tttaatagaa aagttacctc tatctaagga tagaatagag
    10621 ctatatgaac agcttgttaa agagcctaaa attggtatcg gtaacctggt taagtgtatc
    10681 attcgctcag gtaagataag ttacctggca aatcttttga caaccaattt attgaccgtt
    10741 gatattacaa ataaaataaa atttaagttg aatgaacaag ttaaaaaaga aatagtggct
    10801 ttaattaata atgatcagat tgaaccaaac acaacttgta aactattctc taacaatcaa
    10861 gcgattgttt tggcaaccta taaaaaggac aaaaactcac tccaatatgc ggacatagat
    10921 ccggttgtaa cgcttttgca agggaagaat accatcgtac cctctgatgc ttgtcgggct
    10981 tttcctaagg cagaaaaaat agtcatcgca gcctataaat cggatccatt atcgttgcaa
    11041 tttgccgatc ttggtttggt tcaacaatta ttacgaaaag gttctattaa tctcgagcat
    11101 gcttctaaag cttttacaga gagctcacaa ttcaaggagg ccaatgaagc ttatcagctt
    11161 ttaaaatctc tgaagcttct gccaaaaaat gaaaaagaaa ctgagaaagc caagagaatc
    11221 gagttattaa aatcgattga tcaaaagctg gaaattacta atttgagaca agaactttat
    11281 cccttaattt caaatatgtg tattcgatta cttgatgata ttcgaatcct gtcccttgaa
    11341 gatacaataa tcaaaaacac cagattgtcc aagttaatca gttcgaaaca gtgtgatatc
    11401 aatacattaa aaacacttga aaataaaaaa atggaattaa ctaaaacatg ttctctggca
    11461 aaatcaaatt ttactttatt tggagctaca gataataaag ttgatggagg aaatgctaat
    11521 actcataacc acatcactcc agagaataaa taaaagtgtt ttgttggaat tggtcgtata
    11581 gatgcggcca atttgttaaa ttgaattagt ttcggcttaa tctgacacag ttatttaaac
    11641 actgctatca agctgattgg gccttatcag aataatctga tggcagcatt aagatgatca
    11701 aaaacacaac cattcttcta attagctaat ttttataatt gatatttacc atccagaggt
    11761 ttctgcaagt ttgcagaaac ctcgcaaaaa ataatattta tctatcagtt accgagttag
    11821 gtaaaagtat aggataaatc gttttcattt atattattat tcgtctcatt atttaaatat
    11881 acatcggata gcattaacct aatgtttttt gttaatggtt gttgtaaatt attcattaag
    11941 ttttttaatt gtattaaata agtaaatcct ttttctttta gataatcgct acacttttgt
    12001 gtcaagacaa tttctttatg aaaatcacaa tcttcaaatt ctaatcctac atattttcct
    12061 agaaaacgta actctttttc aaaaatatta ttgtttttac atccaaaagc ataatattta
    12121 agataagcag gattttcatt caagttgcta atgcaaattt tatttccttt aagagtaaga
    12181 ttatttgcta tatgttttaa aaattcgatt tcagtataat ttaaattata cttacctcca
    12241 tcatgcgcct tctgtcctat agaaggccta aataagttat caattttata ttcaaaaata
    12301 cgattaatta ttttttccca aggaattgaa gagtttcctt taagtaaatc tcttttaatt
    12361 tcgttaacaa tacttaattg ctgagaagga ctcaaatcag tgagtaatag ataagggttt
    12421 ttgtttgtcg aaattaattg atttaatttt gtattgttta ggcactcaga aatagttaaa
    12481 tgttcaattt cacaaaacgc ataatatttt tttaggttat ctattacagg aaggtagatt
    12541 tcaggaatat tagaagcatt gatgaatcct tgtaaaaaag gatgcttatc tgaaaatttt
    12601 ctttttctta tttctaatgg attaatgccc atctcttcat cccacatagc aatatagtgt
    12661 gagtcgtcaa aaaattccat caggcatatt tttaagtttc catgttcatc aatgctctgt
    12721 ttgattgatt tttttgaaaa acgagcttct tttgtttgtg taagataatt atcaaatact
    12781 gcaatgattt gtagcgctaa tgatgggttc aggggtaatt cgtaggttaa cgatgttagt
    12841 cgataggtat tttcttttgt gtactcaggg atcagtgatg atattgtttc agatatatag
    12901 gctgctttaa aatcgataga atcagattta ttaattttta tatttaaatc attaaatagg
    12961 gggaatttag ttgcgtcttc tttctggaaa gaaagaatgg gtaatgaggt atctatattt
    13021 tttggttcaa atctaaaact tccatcttct tttgtgtgaa atttgtccaa gttaataagt
    13081 attttttctt tct
//
LOCUS       DACTJE010000086        12881 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.086, whole genome shotgun sequence.
ACCESSION   DACTJE010000086 DACTJE010000000
VERSION     DACTJE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12881
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.086"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            129..2444
                     /locus_tag="I9760_001845"
     CDS             129..2444
                     /locus_tag="I9760_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAT5043611.1"
                     /translation="MKENVSHLPRELSRMVFWSLTCLKKAIAEVYGKETYERIEQIRL
                     SMQDTIGSESFSLGKALHSLQIELSKLEKKQLYQIAHGFSLMMELINTCENAYRIFRI
                     NQRKEQVYSDMPQGIHYVLTAHPTEARTNEFLKLFKEIRDYLIEVLKSPYLMNESHLD
                     QMLKISLQINISYHEKPSVEDEAQYIYQYALHPLNIKLYHSFLAKGVPIQLRTWVGGD
                     KDGHSGVNEKIMLKSLELSRSFFIAYIQECLEEVLEIIKLSTALGKNKSLADQINQLV
                     KKAYGLKKIQSKDHEKISAFKNELTATQINALKFLNFEPEQFSIISSIFHLYPALVMP
                     IELREDSAVVKESLTSIKKTTITKMLEQVHSITHGTDPKNYIRGFILSMVESAEDIKN
                     GINLVKKVFKSYALPVVPLFENQLALTNADTILNNSITEDIRQKHLKQWEGNYEVMLG
                     YSDSSKENGVLPSRLMIAKSLKNIQSTLENKNLRPIFFHGSGGSIERGGGDIKEQTAS
                     WSKEMMSNYKVTVQGEMVARLFGSSSILKSQIDKFLDIYSQKGKSNNNDYPEELSLFS
                     TRVSQKYKTLIQSDWFWQIVEQASPYHFLKELKIGSRPTKRKSGPDQRKLRAIPWILC
                     WTQTRLLFPTWWGIGSTWSELNGIEKNRLKALYKENALFAAFVKQLGVTLSKVYLPVW
                     EQYLLQLTGSIEYLRPFQSELESTIQFFYQITDEKDFCFHQPWLGESIQLRSTLIHPL
                     NLIQIEAMKRKDLPLLRKTVTGISCGMLTTG"
     gene            2598..3080
                     /locus_tag="I9760_001846"
     CDS             2598..3080
                     /locus_tag="I9760_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAT5043612.1"
                     /translation="MSFSMKIKNIPYYVATRVVKRIQSWLGLNTLGARAIVTNAEGHV
                     LLVKHTYQPHWYLPGGGVKKGESTKAAVIRELHEEVGLVVAEQDVILFGIYHHKYLGV
                     NDYPVIYIVKNFTSHVTHSGEIEQIGWFSLDALPEMVSPGTKRRLGEYFDNNPISEKW
                     "
     gene            complement(3131..4384)
                     /locus_tag="I9760_001847"
     CDS             complement(3131..4384)
                     /locus_tag="I9760_001847"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAT5043613.1"
                     /translation="MNTNIANQLRAAKKATTDLNLIQSDTRTIILKTLAANLEKHIEN
                     IIQENQKDLSLMLEQDPRYDRLLLNKERILSLVNDVRKVANLPNPLGVNLFEKSMPNG
                     LSIKKITVPLGVIAVIYESRPNVTIDIFSLCFKSGNVCILKGGKEAHFTNSYLLLLIK
                     NTLKNFNINTDIVCLLPPERALMTQLLNATGLVDLCIPRGSQNLINFVRDNAKIPVIE
                     TGAGIVHTYFDKSGDLGKGKKIINNAKTRRVSVCNALDTLIIHADRLKDLPELVETLS
                     QKNVIIYADQDAYHVLDKNYPEQLLIKAKPQDFGHEFLDYKLAIKTVPNIKAAIDHIQ
                     QFSSHHSEAVIAEDESAIDKFLTEVDAAAVYANASTAFTDGGEFGLGAEIGISTQKVH
                     ARGPMGLEALTSYKWVIRGTGQIRD"
     gene            complement(4393..5463)
                     /gene="proB"
                     /locus_tag="I9760_001848"
     CDS             complement(4393..5463)
                     /gene="proB"
                     /locus_tag="I9760_001848"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAT5043614.1"
                     /translation="MKIIIKVGTQSILSVDGSPIYSVLNNLVNQIIYLKNLGHQVILV
                     SSGAVGSGRKIAKNSLGKTYGDTVGEKQLLASIGQPELMNLYSSLFKNENILVSQLLL
                     TKQDFHTRQHYLNIARLLTELLKHQNIIPIVNENDSVAIEELMFTDNDELAGLIAAQI
                     NADYLILLTNVDGVFTSHPNDPESKLINTIDPNSGWPNVSAVKSKLGRGGMVSKLDTA
                     RKMAGLGINTVIANVNVESIVPKILNREAVGTIILASKRKSSIKRWIAFSHRTSLPSI
                     VINDNLITLLRNGNRVMSILPIGIEDFRGECKKGDLIDILNSNNEKIGVGIARYDFLR
                     LKDYLRLQNKPAFIHYDQLHIL"
     gene            complement(5894..6841)
                     /locus_tag="I9760_001849"
     CDS             complement(5894..6841)
                     /locus_tag="I9760_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT5043615.1"
                     /translation="MTQWFVKDLSQLTSVSVQTLHHYDRIGLLKPSLRLANGYRVYSE
                     KDLLKLQQIIALKFFGFELSQIKTLLAEEGNALNHFNSQARVLEQKASTLLDGAKTLR
                     RIIDSVDKNQSIPWETIIQLIEVYKMTEHLEHGWVKEIFTPDELKQYAEFEKEMKANA
                     TPEQKAAMENNWSQLVDEVKNNLKQDPDSKIGIYLGKKIMDWVNGVYGKKYAHLRTKK
                     FEQGFGEGKGLDEVGLTPQIVSWMDKAMDAYWRDRIYGILDQVGKAPSASVLDLWNEM
                     LVDMYGEESSRKNVIYEIALNDDQVSPEAKAWLRNILDS"
     gene            complement(7092..7844)
                     /locus_tag="I9760_001850"
     CDS             complement(7092..7844)
                     /locus_tag="I9760_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT5043616.1"
                     /translation="MAYTVKQLAKISNVSVRTLHWYDEIGLLKPAYQGANGYRYYEEK
                     QCLKLQQILFFRELGFPLNDIQKLLMQNDFDHIKALRAHKQVLESDINRKNQLIVTID
                     KTIQHLKGKHKMSNEELYYGFDSLRQKQYEKELVKEGVVSQDWMNNYKKKTSQWTQAD
                     KDRFIREGKDINQALILAINNQLKPASPEVQAIIHRHHAWVGWNPNKEGYIGLSQRYL
                     TPEFRKLYERQHPDLLEFIIEGMLFYANKNLD"
     gene            complement(7910..8437)
                     /locus_tag="I9760_001851"
     CDS             complement(7910..8437)
                     /locus_tag="I9760_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5043617.1"
                     /translation="MIQIEIVIKTLELSDIPILVDAFQKANWEKPASLFEAYYQEQRK
                     SLRIVWLAYSQDQIAGYVTLKWISQYQPFAHQKIPEIMDLNVLPSFRKQGVGTALLQA
                     AEEKAAGESDVVGIGVGLYGGHDGGYGQAQRLYVNRGYIPDGLGVTYSYKPTVPGQIY
                     PLDDDLILWFTKKLR"
     gene            complement(8434..9390)
                     /locus_tag="I9760_001852"
     CDS             complement(8434..9390)
                     /locus_tag="I9760_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5043618.1"
                     /translation="MSVKQEQCLIKVVSYDSNWPVQFEQEAERIKKALGSNCIEIHHI
                     GSTSVPDLVAKPVIDMIPVVLDISKVDGANAAIRALGYEAKGEYGIPFRRYFQKGDNR
                     LTHHAHVFELGNPEIERHLKFRDWMRMHPKDREAYARLKQELARRYSDDITAYCLGKE
                     DFIATIDKKAGFNGLRMVKALTTREWDKVRHFRQFYFFDKAGLSDPYTWTFEHNAHVH
                     FVLFQGSEITGYAHLQLWSHNRAALRIIVIDELKRNHQYGTRFLALCEKWLKSQGYQS
                     LHVESSPDALKFYRNNGYIDMPFDDPDGYEGDPRDTAVGKIL"
     gene            complement(9394..11175)
                     /locus_tag="I9760_001853"
     CDS             complement(9394..11175)
                     /locus_tag="I9760_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5043619.1"
                     /translation="MTNRLPNHLGSFMWHFLKPYRGIVILFILFALLAGFWGPFNSLL
                     IKSFINTLAEKTSQDLSSLYWIAGLLVLNFIVFDNITWRTLGYLNYKYQAIIKNQIIS
                     QTFEYVLGGSTQFFQDNLSGRIADQITTLADNLEIILHRVSVDFLRGASLLVVSFITA
                     YFVNVLFFYILFLWFVAFASFSIWMSARLVQLSDDHASSESQLSGQLVDSLANQSNIR
                     IFSRKIYEVKRMNTFFRLVQQAFQRKELFIVLLCCAQGGMIAVMMGLASITLIYLYGK
                     GLVSIGDFALILGLSMELGHMMWYTMYQVDQFNQALGKARQSLNALVIPHDIKDKSNA
                     SQLVVTQGRIEFSKVKFHYQGGYSLFQNKSVTIEPGQKVGLVGYSGSGKSTFVNLILR
                     LYEVVDGQILIDGQNLAEVTQESLRQAIAMIPQDPTLFHRSLMDNIRYGRTDATDEEV
                     ILASRKAHSHEFISLLPEGYKTLVGERGVKLSGGQRQRIAIARAILKNAPILMLDEAT
                     SQLDSITESNIQESLWDLMQGKTTLVVAHRLSTLLHMDRILVFDKGHIIEDGTHTELL
                     NRGGLYKTLWDAQVGGFLPDQQNEKGE"
     gene            complement(11168..12617)
                     /locus_tag="I9760_001854"
                     /pseudo
     CDS             complement(11168..12617)
                     /locus_tag="I9760_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
BASE COUNT         3986 a         2558 c         2241 g         4096 t
ORIGIN      
        1 gttgtgccaa aatcctttga gaatcaacta gagaagtaga caatgaagta tttactgcat
       61 cagagttaga taaccaacag acggcattcc cctacagaat aaaccattcg gataaaatgg
      121 agttttcgat gaaagaaaat gtgtcccacc tgccaagaga gctcagccgt atggttttct
      181 ggtcacttac ctgtttaaag aaagccattg cagaagtcta tggaaaagaa acatatgaaa
      241 gaatcgagca aattcgacta tccatgcaag acacgatcgg aagtgagtcg ttttcacttg
      301 ggaaagctct tcatagtctt caaatcgagc tttccaaact tgagaaaaaa cagttatatc
      361 aaatagctca tggtttttcc cttatgatgg agctgattaa tacctgtgaa aatgcctacc
      421 gcatttttcg tatcaaccaa agaaaagaac aagtttattc tgatatgcct caaggtattc
      481 attatgtatt gacagcacac ccaacagagg ctcgtaccaa tgaattttta aagcttttca
      541 aagaaattcg tgactatctt atagaagttc ttaaatcccc ctatctgatg aatgaatctc
      601 atcttgatca aatgcttaaa atctcacttc aaattaatat ttcctatcat gaaaaaccaa
      661 gcgtggaaga cgaggctcaa tacatctatc agtacgcact ccatccactc aacatcaaac
      721 tgtatcattc atttttagca aaaggggtcc ctattcaatt acgcacctgg gtaggaggtg
      781 ataaggatgg gcactctggt gttaatgaaa aaatcatgct taaaagccta gagctttctc
      841 gttctttttt tattgcctat attcaggaat gcttagaaga agtgctggaa atcattaagc
      901 tttcaactgc attaggtaaa aacaaatcct tggcagatca gattaatcag cttgttaaaa
      961 aagcgtatgg tcttaaaaag atacaatcaa aagatcatga aaaaatatcg gcgttcaaaa
     1021 atgaattgac tgctacacag ataaatgcac taaaattttt aaatttcgaa cctgagcaat
     1081 tctccatcat ctccagtatt tttcatctgt atcccgcttt ggtcatgcct atagagctta
     1141 gagaggactc agccgttgta aaagagagtt taacaagcat aaaaaaaaca acaattacta
     1201 aaatgcttga acaggtacac tctataactc atgggactga tcctaaaaac tatatacgcg
     1261 gttttatcct tagtatggtt gaatcagccg aggatataaa aaatggaatt aatctggtta
     1321 aaaaagtttt taaaagctat gccttgccag ttgtgccctt gtttgaaaat caacttgccc
     1381 tcactaacgc agatacaata ttaaataatt caatcactga ggatatcaga cagaagcatc
     1441 tcaaacagtg ggaaggaaac tatgaagtta tgttgggtta ctccgactcg tccaaagaaa
     1501 atggagtact gccctctcgg cttatgattg cgaaaagttt aaaaaacatt cagtctaccc
     1561 tggaaaataa aaaccttcgt cctatctttt ttcatggttc gggcggtagt attgagcgag
     1621 gaggcgggga tatcaaagaa caaactgctt cctggtcgaa ggaaatgatg tctaattata
     1681 aagtcactgt ccagggagaa atggtagcaa gactctttgg ttccagttcc atactcaaaa
     1741 gtcagattga taaatttctt gatatctatt cgcagaaagg caaaagcaat aacaatgatt
     1801 atccggaaga gttgtccttg ttttcgacaa gagttagcca gaaatataaa acacttatcc
     1861 aaagtgactg gttttggcaa atagtagaac aagcctcccc ttatcatttt ctgaaagaat
     1921 tgaaaattgg ctctcgtcca accaaacgaa aatcaggccc tgatcaacga aagcttcgcg
     1981 ccatcccatg gattctgtgc tggactcaaa cacggcttct ttttccaacc tggtggggta
     2041 taggttccac ttggtcagag ctaaatggca ttgaaaaaaa cagacttaag gctctttata
     2101 aagaaaatgc tctttttgct gcttttgtaa agcaattagg tgtaaccctc tccaaggttt
     2161 atctacccgt ctgggaacag tacctgcttc aattgactgg ctctattgaa tacctaaggc
     2221 cttttcaatc agaactagag tcaactatcc agttcttcta tcaaataacc gatgagaaag
     2281 atttctgttt tcaccaaccc tggcttgggg aaagtattca attacgatca acattgattc
     2341 atcctttaaa tcttattcag attgaagcca tgaaaaggaa agacttacct cttttaagaa
     2401 aaacagtcac aggcatttct tgtggtatgt tgacaacagg ataatagaag caaacagcgt
     2461 tattgatgcc actctaatag agtggcgatg ttattgtaaa actaatgaaa tttgaactta
     2521 aaagatatcc agtgctcgtc atggcaagta ttctgtaaag aatagggata agttgagaaa
     2581 tataaaatat acaataaatg agtttcagta tgaagataaa aaatatccct tattatgtcg
     2641 ctacccgtgt cgtgaaacga atacagtcat ggttgggatt gaatactctg ggtgcacgag
     2701 caatagtgac gaatgcagag ggacacgtgt tattggtaaa acatacctac caacctcatt
     2761 ggtatttacc gggaggcgga gtaaaaaaag gagaatcaac caaggctgca gtaatccgtg
     2821 aactgcacga agaagttggt cttgttgttg cagaacaaga cgtgatttta ttcggcattt
     2881 atcaccacaa atatcttgga gttaatgatt atccagtaat ttatattgtt aaaaacttta
     2941 caagtcatgt aactcactcg ggggaaattg agcagatagg atggttttcc cttgatgcat
     3001 taccggaaat ggtgagcccg ggaaccaaac gcagactcgg tgaatatttt gataacaatc
     3061 caatatcaga aaagtggtga ttattgcagt tgtcattgtt gggtggggtg ctgagaaaat
     3121 atggttatgc tcaatctcta atttgtcccg tccctcttat cacccatttg tagcttgtta
     3181 aagcctctag tcccatgggt cctcttgcat gaactttttg cgtactaatt ccaatttcag
     3241 cccctaatcc gaattccccg ccatctgtaa acgcagtaga agcgttggca tagacagctg
     3301 ctgcatccac ttcagttaga aatttatcaa ttgcagactc atcttctgca ataacagcct
     3361 cactatgatg ggaactgaat tgttgaatat gatcaatagc tgcttttata ttgggtactg
     3421 tttttattgc taatttatag tctagaaatt catggccaaa gtcttgtggt tttgccttaa
     3481 ttaaaagttg ttcaggataa ttcttatcta aaacatgata tgcatcctgg tcggcataga
     3541 taatcacatt cttttgagac aaggtctcca caagttctgg caaatctttt aaacgatcag
     3601 catgaataat caaagtatcc agcgcattgc aaacacttac tcttctggtt ttagcattgt
     3661 taataatttt tttacctttt cccaaatctc cacttttatc aaaataggta tgcacgatac
     3721 ctgcccctgt ttcaatgaca ggtattttgg cattgtctct gacaaaattt attaaattct
     3781 ggctccctct tggaatgcat aaatcaacaa gacctgtggc atttaataat tgagtcatca
     3841 atgctctttc tggtggtaat aaacaaacaa tatctgtgtt tatattaaaa ttctttaatg
     3901 tatttttaat taataaaagc agataactgt tagtaaaatg tgcttctttt ccacctttta
     3961 aaatacaaac gtttccagat ttaaagcata ggctgaatat atcaatagtt acattaggtc
     4021 ttgactcata aatgactgcg atgacaccta aaggtactgt gattttctta atactcaaac
     4081 catttggcat tgatttttca aagagattaa ctcctaatgg gttaggtaaa ttggctactt
     4141 ttctcacatc attcaccaat gacaaaattc tctctttatt taacaataaa cgatcatatc
     4201 tgggatcttg ctcaagcatt aaacttaaat ctttttgatt ttcttgtatt atgttttcaa
     4261 tgtgcttctc taaattagcc gctaatgttt tcaaaattat tgttcgggta tcactttgta
     4321 ttaagttaag atcagtagtt gcttttttag cggcccttaa ttggttagca atattagtat
     4381 tcattttgat acctatagaa tatgcaattg atcgtaatgt ataaatgcag gcttattttg
     4441 aagtctaaga tagtccttta atcttaagaa atcatacctg gcgattccaa ctcctatctt
     4501 ttcattgttg gaattaagga tatcaattaa atcccctttt ttgcattctc cacgaaaatc
     4561 ttctatccct attggtaaaa tgctcattac acgattacca tttcgtaata aggtaattaa
     4621 attatcatta attactatag atggtaagga agttctatga ctaaaagcaa tccatctctt
     4681 tatactggat tttcttttac tagcaagaat tatggttcca actgcttccc tatttagaat
     4741 ttttggaaca atggattcta catttacatt tgcgatcact gtattgatac ctaaaccagc
     4801 catttttctg gcagtatcca gtttggatac cataccacct cggcctaatt ttgattttac
     4861 agcagagaca ttgggccaac ctgaattggg gtcaattgta ttaatgagtt ttgattcagg
     4921 atcattggga tgagatgtaa acacaccatc gacattggtt aagagtatta gataatcagc
     4981 attaatttga gctgcaataa gtcctgctaa ttcatcatta tcggtaaaca ttaattcttc
     5041 aatggctacc gaatcatttt cattaacaat agggataata ttttgatgct ttagcaattc
     5101 tgttaataaa cgggcaatat ttaaataatg ctgtctagta tggaagtctt gtttggtaag
     5161 taataactga gatactaaaa tgttttcatt tttaaacaag ctagaataga gattcatcag
     5221 ttcgggttgg cctattgaag ccaagagttg cttctctccc actgtatcgc catatgtttt
     5281 tcccagagag ttcttagcta tttttcgacc agagcccaca gccccagagc ttactaaaat
     5341 gacctgatgc cccaggttct ttaaataaat aatttgattt acaagattat tgagtacgga
     5401 atagattgga cttccgtcga ccgataaaat actttgtgtt ccgactttta taataatttt
     5461 catttcatat ttctaaacaa gcttttctta atatcaagaa tatcagtatg ctttaaattt
     5521 gtctttccaa taatcataaa gatcccataa tacttattct gggcaaaaac catttttttt
     5581 atcgctatgg gtatatacta ttttagttac cttcaaacat gaccacacca gattcaaact
     5641 aaggtttatt aagggcatta caagatctgc aacaagttct aattgataat tcatttataa
     5701 cttttcctta aaatattaaa ttatggcatt aaacagaagt ttgaaaatca tggtttattg
     5761 tgtctatagc aattaaattt atttagagac gattggtctg cctaagatgc taaatcaatt
     5821 aataattgaa ggtatcaaat caagttgaat ggatcggtaa aacaataccg atccaacagt
     5881 gttattgata tttttaggaa tctaaaatat ttctaagcca ggcttttgcc tcagggctaa
     5941 cctgatcgtc atttaaagcg atttcatata tgacattttt tcgtgacgat tcctcaccat
     6001 acatatcaac cagcatttca ttccaaagat ctaatacgct tgctgaagga gcttttccta
     6061 cttgatcaag aatcccatag attcggtctc tccagtaagc atccatcgct ttatccatcc
     6121 aggatacgat ttgtggtgtt aatccaacct catcaagacc ttttccttcc ccaaaaccct
     6181 gttcaaattt tttggttctt aaatgggcat attttttacc gtagacccca ttaacccaat
     6241 ccataatctt ttttcctaaa taaattccta tttttgaatc tggatcttgc tttagattat
     6301 tttttacctc atcaaccagc tgggaccaat tgttttccat agctgctttt tgctcaggcg
     6361 tggcattggc tttcatttct ttttcaaact cagcgtactg ctttaattca tccggcgtga
     6421 atatttcctt tacccagcca tgctcaagat gttctgtcat tttatatacc tctataagtt
     6481 gaataatggt ttcccatggg atagattgat ttttgtcaac agagtcaatg atgcgcctca
     6541 aagttttagc tccatcaagt aaagtggatg ctttttgttc taacacccga gcttggctgt
     6601 taaaatgatt gagcgcatta ccttcttcag cgagtaaggt tttgatttgt gacaactcaa
     6661 aaccgaaaaa cttcaaagca ataatctgtt gtaatttcaa taaatccttc tcagagtaaa
     6721 cacggtaccc attggcaagt cgtaatgaag gctttagcaa tcctatacgg tcatagtgat
     6781 gtaacgtttg cactgataca ctagttaatt ggcttaaatc ttttacgaac cattgtgtca
     6841 ttgtgtttcc cccttcaaga caaaggtaat gcctatagca acaatagggt caagcagttt
     6901 ttaaaatttc ttgaatcatt atttttctaa cctaccaaga gattaattat taagcaaaaa
     6961 actcacaaaa gctcctggtt tggaacaaca tgataagctg gtatgcataa atcagtatta
     7021 tttctgctaa cacgcacaat gctctcaatc caatatatat agggctgata agacgcagcc
     7081 cttctctcag cttaatcaag gttcttgttt gcataaaaca gcatcccttc aataatgaat
     7141 tcgagtagat caggatgctg ccgctcataa agctttctaa actcaggagt caaatatctt
     7201 tggcttaacc caatataccc ttccttgttt gggttccaac ctacccaagc atggtgccta
     7261 tggataatgg cttgtacttc tggagaagca ggcttaagtt gattattaat agccaaaatc
     7321 agtgcctgat tgatatcttt accctcccga ataaatcggt ctttatcagc ctgagtccat
     7381 tgacttgttt ttttcttgta gttattcatc caatcctggc taaccacgcc ttctttaact
     7441 aattcctttt cgtattgttt ttgccgcagt gaatcaaagc cataatataa ttcttcatta
     7501 gacatcttat gcttaccttt taaatgttga atggttttat caatcgttac aatcaattga
     7561 ttttttcgat tgatatcgct ttccaaaacc tgtttatgcg ctctcaatgc tttgatgtgg
     7621 tcaaaatcat tttgcataag cagtttttga atatcattca aggggaatcc cagctctctg
     7681 aagaataaaa tttgctgtaa ttttaagcac tgcttttctt cataataacg atagccatta
     7741 gcaccttgat aggctggttt taataagcct atttcatcat accaatgtaa agtgcgcaca
     7801 cttacattcg atatttttgc taattgtttt accgtataag ccattaaact gtcctctcaa
     7861 atgattccat ttaaagataa gtgctcacgt aacgtaaggg tcaagcattt catcttaatt
     7921 tttttgtaaa ccataaaatg agatcatcat ctaaaggata gatctgccct ggaacagttg
     7981 gcttataact ataagtgaca cctaaaccat cagggatata acctcgattg acataaagcc
     8041 gttgagcttg cccatatcct ccatcatggc cgccatataa accaacgcca atccccacaa
     8101 catcactttc gcctgctgct ttttcttcag cggcttgcag taaagcagtc cccactccct
     8161 gtttacgaaa agagggcaat acatttaaat ccataatttc tggaattttt tgatgtgcga
     8221 acggctgata ttgcgatatc cattttagag taacgtaacc agcaatttgg tcttgagaat
     8281 aagccaacca tactatccga agagactttc gctgttcctg gtaataggct tcaaacaatg
     8341 aagcgggttt ttcccaattt gctttctgaa atgcatcaac cagaatgggg atgtcggata
     8401 gctccagtgt tttaatgaca atttctattt gaatcataat atttttccta ctgcagtatc
     8461 ccgtggatca ccttcatagc catcgggatc atcaaaaggc atatcgatat aaccattatt
     8521 tcgataaaat ttcagagcat ctggtgacga ctcgacatgc aaactttgat agccctgact
     8581 tttgagccat ttttcacata atgccaaaaa acgggtaccg tattgatggt ttcttttaag
     8641 ctcatcgata acgataatcc ttagtgctgc tctgttatga gaccataatt gcagatgagc
     8701 ataacctgtg atttcgcttc cttgaaataa aacaaaatga acatgcgcat tatgctcaaa
     8761 agtccaggta taaggatcag aaagccctgc tttgtcaaaa aaataaaatt gtctgaaatg
     8821 acgcacctta tcccattcac gtgttgttag tgccttaacc attcttaatc cattaaaccc
     8881 ggctttttta tcaattgttg caataaaatc ttctttaccc aggcaatagg cggtaatgtc
     8941 atccgaatat cggcgcgcca actcttgttt taaacgtgca taagcttctc tatctttcgg
     9001 gtgcattctc atccaatccc taaactttaa gtggcgctcg atttcggggt ttccaagctc
     9061 aaaaacatga gcatgatgcg ttagtcgatt atctcccttt tggaaataac ggcgaaaggg
     9121 aataccgtat tctccttttg cttcatagcc aagtgctcgt atggcagcat tagctccatc
     9181 tactttgcta atatctaata caactggaat catatcgatg actggttttg caaccaaatc
     9241 aggtactgaa gtggaaccaa tatgatgaat ttcaatgcag ttactgccta acgctttttt
     9301 aatccgctct gcttcctgtt caaattgcac aggccaattc gaatcataag aaaccacttt
     9361 aatgagacac tgctcttgct tgactgacat tacttattct cctttttcat tctgttgatc
     9421 tggcaaaaaa ccaccaactt gagcatccca caaggttttg tacaaaccgc cacgattcaa
     9481 tagttcagtg tgcgtaccgt cttcgataat atggccttta tcgaagacta aaattcggtc
     9541 catatgcagg agggtagaca agcgatgagc aactaccagg gttgtttttc cctgcattaa
     9601 atcccagaga ctctcttgaa tattggattc ggtaatggaa tcaagctgag atgtggcttc
     9661 atccagcatt aaaatcggag cattttttaa gatggctcgg gcaatcgcaa tgcgctggcg
     9721 ctgaccacct gatagtttaa ccccacgctc ccctaccagg gttttatagc cttctggcaa
     9781 aagacttatg aattcatgtg aatgagcttt cctggaggct aatatcactt cctcatccgt
     9841 tgcatccgtt ctgccatagc gtatgttgtc cattaagctt cgatgaaaaa gagttgggtc
     9901 ttgaggaatc atagcaatag cttgccttaa actttcttgc gtgacttcgg caaggttttg
     9961 accatcaatg agaatctgtc catccacaac ctcataaagt cgtaaaatca gattcacaaa
    10021 agtcgattta ccactgcctg aatagcctac taaacccact ttttgacctg gttcaatcgt
    10081 tacagactta ttttggaaca gagaataacc gccttggtaa tgaaatttta cctttgaaaa
    10141 ttcaattcgt ccttgggtaa ctactaattg agatgcgtta cttttatctt taatgtcatg
    10201 agggatcacc aatgcattca aactttgcct tgctttgcct aaggcttggt taaattgatc
    10261 cacttggtac atggtgtacc acatcatatg acccaattcc atggacagtc cgagaatcaa
    10321 ggcaaaatca ccaatgctca caagaccttt accatacaaa tagattaaag taatcgatgc
    10381 caaacccatc atgactgcaa tcattcctcc ttgtgcacaa cataacaaaa caataaaaag
    10441 ctcttttctt tgaaaagcct gttgtaccaa gcgaaagaaa gtgttcatac gtttcacttc
    10501 gtagattttt cgtgaaaaga tacgaatgtt ggattgattc gccaggctat ctaccaactg
    10561 cccggaaagc tgtgattcag aacttgcgtg atcatcagac aactggacta atcgcgctga
    10621 catccaaata ctaaatgagg caaaggcgac gaaccagaga aacaaaatat aaaagaacaa
    10681 gacattaaca aaataggctg taataaatga aaccactaat aatgaagcgc cacgaagaaa
    10741 atctacagac acacgatgca aaataatctc aagattatca gcaagggtcg tgatttgatc
    10801 ggcaattcgc ccggatagat tatcctgaaa aaattgcgtg cttcctccta acacatactc
    10861 aaatgtttgg ctaatgattt ggtttttaat gatcgcttga tatttatagt tcaagtaccc
    10921 aagtgttcgc caggttatat tgtcaaaaac aataaagttc aataccaata atccagcaat
    10981 ccaatacaaa gaagataggt cctgacttgt tttttcagcc aaagtgttaa tgaaagactt
    11041 aatcaataag ctattgaatg gtccccaaaa cccggccaat agagcaaaca ggataaataa
    11101 tatcactatg cctcgatatg gttttaaaaa atgccacata aaagagccca agtgattggg
    11161 caaacgatta gtcatgatgc actacctcta attgtttctc attgaaatga tgtatcttcc
    11221 cttgatgaat ctgatacctg gtttcaatgt gaatggattc taaaaagtca tagtcatgag
    11281 aaataaccag cattgcacct tggaattcac gcagcacctg aatgatatga gtgcgtgttt
    11341 ccgttcgtga tctcatctaa aataagtagt ttaggtggat ttgcagcaat caatgccatc
    11401 gataaccttg ccttttcacc accagaaaga tttttgacct taatttctac ttcttcattt
    11461 ttgcgaaata aaaaatcatt taaatgagca cgaatttctg tatgaaatgc atggggcata
    11521 ctggagcgca ttaaatccag gactgtttca ttgaagttca aatgttgata atgttgatcg
    11581 atatagccta ttgtattgca atcaggcaca gcccattccc ctgttctctt aatttgactt
    11641 tcccccaaaa tcgctttgac aaaggtcgac ttacctgatg cattcttgcc atataatgcc
    11701 gctcgttcgc aaccgctaag gtgaaaatga atattttcaa gaatcacaac agcctcatag
    11761 gcaacagaag cgtcttgaat cctgattaag ggtttatggt gcccaagtcc ctttaaccta
    11821 aatttgggct taattacctc cggatagttt agagaagaaa gctcctcgag taattgttgt
    11881 tttttatagt taatgtgact taatcgttta ttgcctgttg taattgctga accgagcttt
    11941 gtatgagatc gaatcgttgg ccatttgcgt tgggttatct tcttttcacc ctgaacacgc
    12001 gcacgtttat tacgctcctg ctctttcatc aaagcaagat gtgattcttt tttctgtcgt
    12061 acaatccttg ataactcctg ttcaatcgat gctttcttgt cttcaagaat ttgttgataa
    12121 tcaaaataag ttcctctgaa tacagtcact tgacctaaat caatatgcca tagagtatct
    12181 gtgactgtat tgatgagttc tatatcatga gaggctataa tcaatgtccc aggataatgc
    12241 tcaagcatgc gcattaaaga acaacgatta cgtctgtcca gatgatttgt gggctcatct
    12301 aacaacaata cattaggctc atacgacagt attttgctta atactcggtt taatcgctga
    12361 ccgccactta aatcaggaca attttcaagc acttgaggta aatgaccaaa gcgaacatca
    12421 tggggtattt taatttcgcc cgcaggggct aaaaaaagac cacaaagcat cttgagtagc
    12481 gttgattttc ctgagccatt acggccaatc agtgcaatgc gatcagcgaa atggatttca
    12541 ccactaaagt cttgaaaaca ggttttatgc ggatagataa gactgaggtc tttaaattga
    12601 atcggtttat ggttcatgat ttactcttaa gcatgtctgc caggacagta aaaaactaaa
    12661 agtcacaaag gactttgttt actttgccct atagttgggc gcacttaaga tgtaattttc
    12721 atgacggcct aaacctacct tttaactggt aaaaaattac agggaatata aattaaaata
    12781 gttgaagatg tcaatattat attcaaaatt attttttcct ggcttatctt ttccttttat
    12841 actctatata taaatgactt tatcaaccgg gaaatatcac t
//
LOCUS       DACTJE010000087        12716 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.087, whole genome shotgun sequence.
ACCESSION   DACTJE010000087 DACTJE010000000
VERSION     DACTJE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12716
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.087"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..457
                     /locus_tag="I9760_001855"
     CDS             <1..457
                     /locus_tag="I9760_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043620.1"
                     /translation="EFQIQLRVNILFSRFLKSLDEQQRRKLILTLSLFDPVEAKSKFL
                     TNCIHYVTNRLCQISMHQLDSSPNFFGTYQCIDSKKLLINKTDVKQVSAILEAFKEML
                     HSLEERCNPEQLENMLIFLRNISRPILTVAEIEEAQQSARVIDYIGAPT"
     gene            618..1787
                     /locus_tag="I9760_001856"
     CDS             618..1787
                     /locus_tag="I9760_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2744 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043621.1"
                     /translation="MSNLIELALRNAALDRTKLLSQVQKIEFLNGYDPRFVEGLISLE
                     MIGDIDEYVASLMYVKMTDKYTQEEKEQLTDTILTINNDRLISCQYRFPQLYQKLMQD
                     QFLSTFFSSSHLLPNIAPGTNNQSYETVSTNNKMKMESAKLYGHYEYYQCVVALITQI
                     DGSRYFYHLNPNRIFGDYMGSAPLKLSGSELKDVKDITFILHPRSMVDYREFQRLQER
                     NIVFKTVMLPVGAKSVSLSYDTDKDVLTVNNLEAGQTVTYNNLLQTPQYNRYQYTPEQ
                     YFMTQLYRGQTWAEALGEALDKYIVTRQEGATWWDLKPLFFNRKTTKNNMIAAARIKK
                     EIEAGATSFDFTGVSRNGLLNQLLTTAVDYQLEYQRGLQNGVLSAEDLTTSAALR"
     gene            complement(1822..2799)
                     /gene="rsgA"
                     /locus_tag="I9760_001857"
     CDS             complement(1822..2799)
                     /gene="rsgA"
                     /locus_tag="I9760_001857"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAT5043622.1"
                     /translation="MSKRRINKQQSARIAKIQKNYHEYMGNNSDNLADGLVITRFSRH
                     AEIEDNQGKRILCSIRPNLDTLVAGDRVIWQIEGEKQGVVVSIYPRGSLLARPSQKGL
                     KRPVAANITQLVVVIAPKPEISWPLLDSYLIMAEILQVHVVIVLNKIDLPCQTLQQRL
                     MMDYQLLNYPVIMASNQTPDTIVPLKQALNNQISVFVGQSGVGKSSLISSLLPHEENI
                     AIDKISMVSELGKHTTRNSRYYHLPSGGALIDSPGVREFSLWDIDCSMIAQGYKEFKP
                     YLSQCKFRNCTHINTPQCAIIDALNKGKISTQRYENFTKLCAQFNSLKE"
     gene            2862..4094
                     /locus_tag="I9760_001858"
     CDS             2862..4094
                     /locus_tag="I9760_001858"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HAT5043623.1"
                     /translation="MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRKYRQVGR
                     DFPVFLHPETKEEYALARTERKSAPGYYGFICDFSESVTLEEDLARRDLTINAMAMDE
                     QGNLIDPYQGQRDLEEKLLRHVSSAFVEDPVRVLRVARFASRFHHLGFRIANETRLLM
                     YSMVKQGELAHLIPERVWQEWQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPN
                     PHQYHQEIDTGIHSLMTLRASSELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEE
                     GTKLIRALCARLRIPNDYRDLAVTVARAHLNIHRLCELRPNTIVKLLEQVDAFRRPQL
                     FHKILIACQADAESCGKTVVYRQTQLWNEILSECVKVTPQTFIVQGYEGKAIKEAMHQ
                     SRVACVERIMTSWKSNEK"
     gene            4084..4647
                     /gene="orn"
                     /locus_tag="I9760_001859"
     CDS             4084..4647
                     /gene="orn"
                     /locus_tag="I9760_001859"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAT5043624.1"
                     /translation="MKNNQNLIWIDLEMTGLEPEQDRIIEMATIVTDPQLNILAEGPV
                     IAVSQPKILLDSMDAWNTKQHNQSGLVKRVLESNVSESQAEQLTIEFLKQYVDKGKSP
                     MCGNSICQDRRFLYKYMPELAAYFHYRNLDVSSLKELVLRWRPELMNGVVKESKHLAL
                     DDIKDSINELIYYRQHFINLPEVKNDK"
     gene            4637..5299
                     /locus_tag="I9760_001860"
     CDS             4637..5299
                     /locus_tag="I9760_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAT5043625.1"
                     /translation="MTNRERLELPNGAKKLLLHSCCAPCSGEVMEALIFSEIDFTIFF
                     YNPNIHPLQEYEIRKEENIKFAQKHNIPFIDADYDKENWFARAKGLEWEPERGKRCTM
                     CFDMRFERTALYAYEHDFPVISSSLGISRWKDMNQINDCGVRAASHYPDLIYWTYNWR
                     KNGGSERMYEIAKREAFYKQEYCGCVYSLRDTNAWRKQNTRDRIKIGLNYYSASKNEG
                     DL"
     gene            5296..6219
                     /locus_tag="I9760_001861"
     CDS             5296..6219
                     /locus_tag="I9760_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAT5043626.1"
                     /translation="MSKHSHDGAHHHEHGEVTYDRAFIIAITANGLFVVMQIIFAYLA
                     NSTSLLADAFHNLGDVLGLILAWVATGLMKRKPTHKATYGFKKTSILAALANGALLVF
                     TCGIIATDAMYKLFSPTEIQAVSVMIVAAIGIVVNFSTALLFLRGSDDLNIRGAYLHL
                     FYDALISVGVVLSAGVLYLTGWLWIDPVVGLLIALVILKGTWSLFADSFRLIIDGVPR
                     DISWIKVSDFLLAIPGVKSIHDLHIWAISTKENALSVHLFMPEESLSDDLRRELVEKL
                     KHDFSIQHVTIQVEKTETECNDACHNPRIII"
     gene            complement(6355..7542)
                     /locus_tag="I9760_001862"
     CDS             complement(6355..7542)
                     /locus_tag="I9760_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HAT5043627.1"
                     /translation="MIRVLFAFIILLLSVFLGIQLNKDPGYVLIAINNWTIETTLWVS
                     VFGLFFLFFLIYGLVQFCNKITRMPGMVNKWHSRRLAQKAQATTRKGLIEYSEGYWQK
                     AKNHLIQALPNTDTPLLNYLTAARAAQKMGDSKLRDHYLREAQQSMPEAKIAVELTQA
                     QLQLANHQWEQALATLKHLQDLAPRHPYVLKLLMNLYEEIKDWQQLIALLPDLKKNHI
                     ISDQAFEQLQKNTYLQAMIDLAKHNQSDGITKLYQTLPKTLVNDTAITAEYARFLIRN
                     QDYDQAEYHLKRCLRKELDNRLIELYSLLPCEENQLLFAESLLKKNPHSAALYLCLGR
                     MCLSRHLWGKAKHYLEQSIELNPTPVAFIELGKLHEKLNDPLSSGSCYKKGLELITKD
                     ELS"
     gene            complement(7545..8669)
                     /locus_tag="I9760_001863"
     CDS             complement(7545..8669)
                     /locus_tag="I9760_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043628.1"
                     /translation="MANSNEEQKKTIQPQQPSKVEPDINQQMKASFFGSKYLSLIISL
                     TSLLAFLAIIVSIYSIQLEKQFQNHQLVENKNLTDELERIKVEQNTVQKLLDNNANHL
                     QQIQSDLTSKMDSLNKELQTAMKQKLYQNNDWLLLKARYYLELAQINVHWSDNFNTSV
                     ALLQQADALLKGMNIPKIFTIRQTIAKEIAQLKSISVVDITGILSQLDAAQAAINNLS
                     IQSVVDQQEVLNHITKSEESGKTGWRNRLQDSVNFLEKLVVIRRHNENIQPLISPLYE
                     SAIKENIRLNLQEAQWAILNNNPAVYQFALNQAIANLKRVFNESSQNTDVLIKQLSTL
                     QNVKLTQEKPVVGQAIPLINQMIDNKELLVDQANSKKGEN"
     gene            complement(8673..9425)
                     /locus_tag="I9760_001864"
     CDS             complement(8673..9425)
                     /locus_tag="I9760_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAT5043629.1"
                     /translation="MTKSLNGLRILNTRPREQAQVLNKKIIESGGIPIDCPTMEIMAS
                     ETNWINNLPDLTSVNQAIFVSTNAVRYFMMELASKNINWPNSIRVTAIGKGTSRVLNE
                     FNIQVSDVPELPDSEHLLTLNSLQQIRNQTILLVKGEGGRQLIEEYLMLKGAKLCLLS
                     VYKRVMPAIDQEFINSLWRDDLVDIILLTSEQSIHNLFKMFSIEGQMWLQNKPCLVIS
                     DRLAKVASLFGIKKILISHPERMIGALFDYKD"
     gene            complement(9422..10351)
                     /gene="hemC"
                     /locus_tag="I9760_001865"
     CDS             complement(9422..10351)
                     /gene="hemC"
                     /locus_tag="I9760_001865"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027223811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAT5043630.1"
                     /translation="MSAKIIRIATRQSPLALWQANHVREMLVKQWPNLSIELLPMITS
                     GDRFLKDKLLSAGGKGLFVKELEEALLDKRADLAVHSTKDMPAQLPDGLLLTAICKRD
                     NPFDALISPQFKSLAALPKNAIIGTSSLRRQSQLLAYNPNLQIKTLRGNIHTRLSKLE
                     SGEYQAIILAAAGLERMGLAHHITQLIPDDIMLPTCAQGALCIECRTDDLEIQELVHG
                     LNDPISALCVHTERRVNAKLGGNCHIPFAVYCTITEEKLLLLRAKVLNMDGSQMIDDE
                     QQGKIAEAEVIADRCTESLMTKGAMSLLSTIPS"
     gene            10743..12032
                     /locus_tag="I9760_001866"
     CDS             10743..12032
                     /locus_tag="I9760_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT5043631.1"
                     /translation="MSQLKKIVKHLDESMQRSLSNSAHQLVAVGAIAFVGFPLFYVIW
                     AFWLPQPYENLPLRLIGSLLGLGLMLTPYWPLKWKQYLSWYWFLTLLFTLPYFFTFLF
                     LMNQASVISAMSLLCGVFLLVLLVDLLSLSIVLILGFSLALVSYYLVSPQMYFGEEHI
                     QMTLVIIFTIIAGSTLNYKTAMLQQQKLAGMAAAAGMIAHELRSPLLGIKSGAQALVQ
                     YLPRLIDGYQLAKKQELISSPLRDRLLLQLGGVSNRIVSEIDYANTIIDMLLVKASRD
                     NILQNCILETCSIAECLSEAITRYPFQSPRERTLITWTGDFQFKGSKLLMQHILFNLI
                     KNALYVIAMAQRGEINIWTESGDKYHYLYFKDTAKGMSKQQLNRLFNHFYTTTFMGTG
                     IGLSFCKLVMNAFGGDIVCESKEGEYALFILSFPGSV"
     gene            complement(12120..12683)
                     /locus_tag="I9760_001867"
     CDS             complement(12120..12683)
                     /locus_tag="I9760_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HAT5043632.1"
                     /translation="MANSQEFNQPPVDELKRNWGWILGFGIFFLILGCVGLGMVVGLT
                     LVSMFFFGALLIVGGISHIIDVFKYKEWKGMIWQALIAVLYIAAGCIVLYDPFLASTL
                     ITAFLAAVLIVIGLTRMIMAFALKDSTGWGWLLLAGITAIILGILILMQWPVSGLWII
                     GLFIAIELIVTGWTYIFIAISVKTAKL"
BASE COUNT         3759 a         2254 c         2557 g         4146 t
ORIGIN      
        1 tgagtttcaa attcaactga gagtcaatat tttattttcc agattcttga agagtttaga
       61 tgaacaacag agacgaaaat taattttaac tttgtcattg tttgatcctg tagaggctaa
      121 aagtaaattt ttaacgaact gcattcatta tgtgacaaac cgactatgtc aaattagtat
      181 gcatcagtta gacagttccc ctaatttttt tggcacttac caatgcatag atagtaagaa
      241 attgttaatt aataaaacag atgtaaagca agtgagtgct attttggaag cctttaaaga
      301 aatgcttcat tcgttagaag agcgttgtaa ccctgagcaa ttggaaaata tgctcatttt
      361 cctgcgaaat ataagccgcc ctattttgac tgttgcggaa atagaagagg cacaacaatc
      421 agcccgagtt atcgattata taggcgctcc gacctgactt aattaactgg aattatccta
      481 attcattatt gtgcattttg aactcatcgc attgatatgg atcgattgtc ttggcctaga
      541 tgagcaaagc ttgctccagt gcgcgaattg aatcataatt aaacaatttg tcttatttat
      601 gcttttgaaa ttataatatg tcaaatctaa tagaacttgc tttaagaaac gcggcacttg
      661 atagaacaaa acttttaagt caggtacaaa aaattgaatt tttaaatggt tatgatccca
      721 ggtttgtcga gggattgata tcattagaaa tgataggcga tattgatgaa tacgttgctt
      781 ctttgatgta tgttaaaatg actgataaat acactcagga ggaaaaagag caattaacag
      841 atactatatt aaccatcaat aatgatcgat tgatttcatg ccaatatcgc tttcctcaat
      901 tatatcaaaa attaatgcaa gaccaatttt tatctacttt cttttcaagt tcgcatttgc
      961 tccccaatat tgctccaggc acaaataatc aatcttatga aacagtaagt actaataata
     1021 aaatgaagat ggagtcagca aagttatatg ggcattatga atattatcaa tgtgttgtcg
     1081 ctttaatcac tcaaattgat ggttctcgat atttttatca tttgaatcca aaccgtatct
     1141 ttggtgatta catgggtagt gcgcctttga aattatcagg tagtgaactt aaagatgtaa
     1201 aggatataac ctttatcctt cacccgcgaa gtatggttga ttatcgcgaa tttcaaagat
     1261 tacaagagag aaatattgtt tttaaaaccg ttatgctacc agtgggggca aagtcggtat
     1321 cacttagtta tgatacagat aaagatgtat tgacggttaa caatcttgag gcaggacaaa
     1381 ctgtaacata taacaatctg ctacaaacac cccaatataa tagatatcaa tatacacctg
     1441 agcaatattt catgacccaa ctatatagag gccaaacatg ggcagaagca cttggcgagg
     1501 cattggataa atatattgtt acaagacaag aaggagcaac ctggtgggat cttaaaccat
     1561 tattttttaa tcgtaagact acaaaaaata atatgattgc tgctgcgcgg atcaaaaagg
     1621 aaattgaggc tggtgctacc tcatttgatt ttacaggggt tagcaggaat ggcttgctta
     1681 atcaattatt aacaactgct gttgactatc aattagaata ccagcgtgga ttgcaaaacg
     1741 gggtgctttc ggctgaagac ctaacaacaa gtgctgcgtt acgttgataa ctgattatct
     1801 attttcggaa gagctttttg tttactcttt taaactatta aattgggcac ataattttgt
     1861 aaaattttca tatcgttggg ttgaaatctt tcccttattt agcgcatcaa tgatcgcgca
     1921 ttggggcgta ttaatatgtg tgcagttgcg gaatttacat tgggacaaat aaggtttgaa
     1981 ttctttatat ccttgagcaa tcatagagca atcaatatcc cacaagctga attcccgaac
     2041 gcctggcgag tctatcaagg caccaccact aggtagatga taatagcgag agtttcttgt
     2101 agtatgtttg cctaactctg agaccatgga tattttatcg atcgcaatat tttcctcatg
     2161 tggcaataaa cttgaaatca atgatgattt gcctaccccc gattggccaa caaaaacact
     2221 gatttgatta tttaatgctt gttttaatgg tactattgtg tctggggttt ggttacttgc
     2281 catgatgacg ggatagttca gcaattggta atccatcatg agtcgctgct gaagtgtttg
     2341 acagggaaga tcaattttat tgagaacgat caccacgtgt acttgaagta tctctgccat
     2401 aatcaaatag ctgtcaagta atggccaact aatctctggt tttggagcaa tcactacaac
     2461 cagttgagtg atatttgccg caacgggtct ctttaacccc ttctgactgg gccttgccag
     2521 taagcttccc ctgggataga tgctcacaac taccccttgt ttctcacctt ctatctgcca
     2581 gattactcta tcaccagcca ccagtgtgtc aagattaggt cggatagagc aaagaattcg
     2641 tttcccctga ttgtcttcaa tttctgcatg cctgctaaat cgagtaatga ctaaaccatc
     2701 agccaggtta tcggagttat ttcccatgta ttcatggtag tttttctgga ttttagctat
     2761 acgggcagat tgctgcttat tgattcgtct tttactcata gtgtcaatct atactataat
     2821 tgatgcgatt aataatacag tatgaaggta aatcatattt aatgaaagtc tacttggtag
     2881 gcggtgcagt tcgtgatcga ttattaggaa ttcctgtcca ggaacaggat tgggtagtcg
     2941 tcggagcaac gccggaagaa ttattaaaaa gaaaatatag gcaagttggg cgggatttcc
     3001 ccgttttttt gcatcctgaa acgaaagagg aatatgcatt agcaagaaca gagcgtaaat
     3061 cagctccagg atattatgga ttcatttgtg attttagtga atcagtgacg ttggaagagg
     3121 atttggcacg acgagattta acgattaatg ccatggctat ggatgaacag ggaaatttaa
     3181 ttgatccata tcagggacag cgagatctcg aagagaaatt attacgtcat gtgtcctccg
     3241 cttttgtaga agatcctgtt cgggttttac gtgttgcccg ttttgccagt cggtttcatc
     3301 atcttggatt tagaatagcc aatgagactc gtttattaat gtattccatg gttaaacaag
     3361 gtgaattagc tcatttgatc cccgagcgtg tttggcagga atggcaaaaa agtcttgaag
     3421 aaaaaaatcc tgaacaattc attctttctt tacgttcctg tgatgcatta agagtgattt
     3481 tgccagaaat taactcatta tttggtgtgc ctaatccaca tcaataccat caagaaatag
     3541 atacgggcat tcattcatta atgacattac gggcatcatc tgagcttagc gaagaaccat
     3601 tagtacgatt tgctgctttg gttcatgatt taggcaaagc ctcgacacca attcaggcat
     3661 ggcctaaaca ccatgggcat gaggaggagg gtaccaagct gattcgagca ttatgtgctc
     3721 gcttgcgcat tcctaatgat tatcgcgact tggccgttac agtggcgcga gctcatttga
     3781 atattcatag gctatgcgaa ttgcgtccta acacaatcgt caagcttttg gagcaggtag
     3841 atgcctttag acgacctcaa ttatttcata aaatattgat tgcttgtcaa gcagatgccg
     3901 agagttgtgg caaaacagtg gtctatcgcc aaactcaatt atggaatgaa atattatctg
     3961 aatgtgttaa agtgacgcca caaactttta tcgtacaagg ttatgaaggt aaagcgatta
     4021 aggaggccat gcatcaaagt cgtgtggctt gtgtagagcg aattatgact tcttggaaat
     4081 caaatgaaaa ataatcaaaa tttaatttgg attgatttgg aaatgactgg ccttgagcca
     4141 gaacaagata gaattataga aatggccacg attgtaacgg atccacaact taacattctc
     4201 gcagagggac ctgtcatagc ggtgtctcag cctaaaattc ttctagacag tatggatgca
     4261 tggaatacaa agcaacataa tcagtcggga ttggtgaagc gagttttaga gagcaatgtc
     4321 agtgaatctc aagctgaaca attaacgatt gaatttttaa aacagtatgt ggataaggga
     4381 aaatcaccca tgtgtggcaa tagtatctgc caggatagac gttttttata taaatacatg
     4441 cctgagttgg ctgcttattt ccactatcgg aatttggatg tcagttcgtt gaaagagctg
     4501 gtattgcgct ggcgtccaga attaatgaat ggagtagtta aggaatcgaa acatcttgct
     4561 ttggatgata taaaagattc aatcaatgag ctgatttatt atcgacaaca ttttattaat
     4621 ttgccggaag taaaaaatga caaatagaga acgtttagaa ttacctaatg gcgctaaaaa
     4681 gttattactt cattcctgtt gtgctccttg ttcaggtgaa gtgatggaag cgctcatttt
     4741 ttctgagatt gactttacta tattttttta taaccccaat attcatcctc tccaggaata
     4801 tgaaataaga aaagaagaga atattaagtt tgcccaaaag cataatatcc cctttatcga
     4861 tgccgattac gataaagaaa actggtttgc tcgagccaaa ggattggaat gggagccaga
     4921 aagaggcaag cgttgcacta tgtgttttga tatgcgattt gaaagaacag cactctatgc
     4981 ttatgagcat gactttcctg ttatcagtag ctctttgggt atttcacgtt ggaaagatat
     5041 gaatcagatc aatgattgcg gagtgcgggc agcaagtcat tatcctgatt tgatatattg
     5101 gacttataat tggcgaaaga atggcggctc tgaaagaatg tacgaaattg ccaaaagaga
     5161 agcattttac aaacaagagt attgtggttg tgtttattct ttacgagata cgaatgcctg
     5221 gcgcaagcaa aatacaagag atcgcattaa aattggactt aattattact ctgcaagcaa
     5281 gaacgaagga gacttatgag taagcattcg catgatggag cacaccatca tgagcatggg
     5341 gaagtgactt atgacagggc ttttataatt gcaattactg cgaatggcct ttttgttgtg
     5401 atgcaaatta tttttgccta tttagcgaac tctaccagct tgctagcaga tgcttttcat
     5461 aatttaggtg atgtattagg gctgatttta gcatgggtag caaccggatt aatgaagaga
     5521 aaacctaccc ataaagcaac ttatggattt aaaaaaacct ctattttagc ggcattggcc
     5581 aatggagcgt tactggtatt tacttgcggg atcatagcga cagatgcaat gtataagtta
     5641 ttttcaccga ctgaaattca agctgtctct gtgatgatag tcgctgcgat tggtatagtc
     5701 gtgaacttct ctacagcgct cttgttttta cggggttctg acgatcttaa tattcgagga
     5761 gcttatttgc atttatttta tgatgctttg atctcggtag gtgttgtttt atcagcgggt
     5821 gtattatacc tgacgggttg gttatggata gatccggttg tcggactatt aattgcatta
     5881 gtgattttga agggcacctg gtcactattt gcggatagtt tcagattaat tattgacggt
     5941 gttccccgtg atatatcctg gattaaagtc agtgattttc tcttggctat ccctggtgta
     6001 aaatctattc atgatttaca tatttgggca atcagtacga aagaaaatgc actttccgtt
     6061 catttattta tgcccgagga gtcattatct gatgacttgc gtagagagct ggttgaaaaa
     6121 ttaaagcatg atttcagcat tcagcatgtc actattcagg ttgaaaaaac agaaacggaa
     6181 tgcaatgacg catgtcataa tcccaggata atcatttaat tggggctgga tttacgaccc
     6241 ctaatttctt tgtttaaaaa aaggtttaat ttattcatta attgtatcta aacgccctta
     6301 ttaaaaacat tttttgcttt gcatttcgag ctgtgtcttt cagtttttaa tcaattagga
     6361 taattcatct tttgttatca actccagacc ttttttataa caagagccac tggataaggg
     6421 gtcatttagc ttttcatgga gtttgccaag ttcgataaat gctacgggcg tggggtttag
     6481 ctcaatagac tgttcaagat aatgtttggc ttttccccat aaatgccgcg agaggcacat
     6541 tctccctaaa cataggtaca atgcggctga gtgaggattt tttttgagta atgattcagc
     6601 aaataacaat tgattttctt cacaagggag caggctataa agctcaatta accgattatc
     6661 caactctttg cgaaggcatc gctttaaatg atattctgcc tgatcgtaat cctgattcct
     6721 aatcagaaag cgggcatatt ctgctgtaat tgcagtatcg ttaaccagtg tttttggcag
     6781 cgtctgatac aatttggtaa tgccatctga ttgattgtgt tttgccaaat caatcattgc
     6841 ttgcaaataa gtattttttt gtaattgttc aaaagcttga tctgaaataa tgtggttctt
     6901 ttttaaatca ggtaaaagag cgatcaactg ctgccaatct tttatttctt cataaagatt
     6961 cattaataat ttcaagacat aaggatggcg tggtgctaaa tcttgaagat gctttaatgt
     7021 ggccaatgcc tgttcccatt ggtgattggc caattgtaat tgcgcttggg ttaattcaac
     7081 agcaattttc gcttcgggca tagattgctg tgcttcccta agataatgat cgcgaagctt
     7141 gctatccccc attttttgag cagctctcgc tgctgttaaa taattaagta aaggagtgtc
     7201 ggtgtttggc agagcttgta ttaaatgatt ttttgctttt tgccaatagc cctcgctata
     7261 ttctatcagt ccctttcgag tcgtggcttg agctttttgt gccaagcgtc gtgagtgcca
     7321 tttattgacc atgcctggca ttcttgtaat tttattgcaa aactgaacca ggccatagat
     7381 taaaaaaaac agaaaaaaca agccaaaaac agagacccat aaggtagttt caatagtcca
     7441 gttattaata gctattaaga cataacctgg atccttattg agttgaatac caaggaacac
     7501 agagagcagt aaaataataa aagcaaacag aactcgtatc attcttaatt ttctcctttc
     7561 ttgctgtttg cttgatcgac cagcaactct ttattgtcaa tcatttgatt gattagcggg
     7621 atggcttgtc ctacgacagg tttttcctga gttaatttta cattttgcaa agtggacaat
     7681 tgcttaatca aaacgtcggt gttttgggaa gattcattaa atactctttt aagatttgca
     7741 atggcctggt ttaaagcaaa ctggtagact gcggggttat tatttaaaat agcccactgc
     7801 gcttcctgaa ggttaaggcg gatattctct tttattgcag attcatatag aggggatata
     7861 agtggttgaa tattttcatt gtggcgacga ataacaacca atttctccag aaaattcaca
     7921 ctgtcttgca accgatttcg ccatccagtt ttgccagact cttcagactt ggtgatatgg
     7981 tttagcactt cctgttgatc tactacggat tgaatagaaa gattattgat agccgcttgg
     8041 gccgcatcca attgacttaa tatccctgtg atatcaacaa cagaaattga ctttaattgg
     8101 gcaatttctt tggcgattgt ctggcgaatg gtaaatattt tggggatatt catacctttc
     8161 agtagcgcat cagcttgctg taaaagggcg acagaggtat taaaattatc actccaatga
     8221 acattgattt gcgctaattc aagataataa cgtgccttta aaagaagcca atcattattt
     8281 tgatacaatt tttgcttcat agctgtttgt aattctttat ttaagctatc cattttgctt
     8341 gttaaatcac tctgaatttg ctgaagatga ttggcattgt tatccaggag cttttggact
     8401 gtattttgct ctacttttat tctttccaat tcatcagtta aatttttgtt ttcaacaagc
     8461 tgatggtttt gaaattgttt ctccaattga atggaataaa tagagacgat gatagcaagg
     8521 aacgccaaaa gcgatgtcaa gctgattatt aaggataggt atttactacc gaagaaagag
     8581 gctttcattt gttggttgat gtcgggctca actttagatg gttgttgcgg ttgaattgtt
     8641 tttttctgtt cttcattgct attggccatg gattaatcct tatagtcaaa taacgcacct
     8701 atcatccttt ctggatggct aataagaatc tttttaattc caaataaaga agctaccttt
     8761 gcaagtcggt cactaatgac caggcaaggt ttgttttgta accacatttg cccctctatg
     8821 ctaaacattt taaaaagatt atgtatggac tgctcacttg ttaacagtat aatgtccacc
     8881 aggtcatcgc gccacagaga attaataaat tcttggtcga tggctggcat gactcgttta
     8941 tagactgaca gaagacatag tttcgctccc ttcagcatta aatattcttc aatcagttgc
     9001 ctgcctcctt ctcctttcac aagcagaatc gtctgatttc ttatttgttg caacgaattg
     9061 agagtcagta aatgctcgct gtcaggtaat tcggggacat cactaacctg gatattgaat
     9121 tcattgagta ctctggaggt gcctttacct atagcagtta cccggatgct atttggccaa
     9181 ttgatgtttt tggaagccag ttccatcatg aaataacgaa cggcatttgt actgacaaaa
     9241 atggcctgat ttactgaggt caaatcaggc aaattattta tccaattggt ttcagacgcc
     9301 attatttcca tcgtggggca gtcgatgggt attccgccag actcgataat ttttttatta
     9361 agcacttgtg cttgctccct gggtctggtg tttaatatgc gaagaccatt aagtgatttg
     9421 gtcatgatgg tatcgtactt aataaggaca ttgcaccttt agtcattaat gactcggtac
     9481 acctgtctgc aatgacttca gcttctgcaa ttttcccttg ttgctcatca tcaatcattt
     9541 gagaaccatc catattaagt acttttgctc tcaaaaggag gagtttctcc tcagtaatgg
     9601 tacaataaac agcaaagggt atatggcaat tgcctcctaa ttttgcgttg acgcgtcgct
     9661 cagtatggac acaaagagca gaaattggat catttaagcc atgaaccagc tcctggattt
     9721 ccaaatcgtc agttcggcac tcaatacata aagcgccctg agcacaagta ggcagcataa
     9781 tgtcatcggg tatgagttgg gtgatgtgat gtgccaagcc cattctttcc aagcctgcgg
     9841 cagcaagaat aatggcttga tactctcctg actccagttt gctgagtctt gtatggatgt
     9901 ttcctcttaa tgttttgatt tgcaaattgg gattgtaagc caatagttga gattgacgtc
     9961 taaggcttga tgtaccaata atcgcgtttt tggggagagc agcaagactt ttaaattggg
    10021 gacttattaa agcgtcaaaa gggttatctc ttttgcaaat ggcggtcaac aagaggccat
    10081 ccggaagttg tgctggcatg tctttagtcg aatgaacagc caagtccgct cttttatcca
    10141 ataatgcttc ttctaactct ttaacaaata accctttccc tcctgcggat aataatttat
    10201 ctttaagaaa ccgatcgcca gaggttatca tgggtaataa ttcaatgctt agatttggcc
    10261 attgctttac aagcatttcc cgtacatgat tagcctgcca tagtgcaagc gggctctgcc
    10321 tcgttgcgat tcgtataatt tttgcgctca tggaatagac ggtatatgtt ctttgggtaa
    10381 agatcataac attgattcta atttaaaaag aaccccattg agataatagg tacgcaaatc
    10441 ttgtcgggca attgatttat aaatgagcat tcctaccggt ttgagaattg gtttatagtg
    10501 ccaaccccca atttctttgc taaaaaattt tttaatcctg tcatttatag tcatttaaca
    10561 atcaaataac tatagcttat ctaagctcct tattaaaaat tttttttgcc tcgaccttgg
    10621 agctttgtgt aagtcctata gagcatatcc catgcacaat ccctatattt tatgataggc
    10681 ttttctggta gataaagcgc tgaaaatcag gggcgttttt tagctggatt taaggataag
    10741 aaatgtcaca actaaaaaaa atagtgaaac atttagatga atcaatgcag cgtagtttgt
    10801 ctaattctgc tcatcaattg gttgcagtag gcgcgatagc ttttgtggga tttcctttat
    10861 tttatgttat ttgggctttc tggttgcctc agccttatga aaacctgcca ctccgtctta
    10921 ttggaagcct tttaggtttg ggattgatgc ttactcctta ttggccatta aaatggaagc
    10981 aatatttatc ttggtattgg tttttaactc ttttgtttac tttgccatat ttttttactt
    11041 ttttattttt aatgaatcaa gcaagcgtta tttctgcgat gtctttgttg tgtggtgtat
    11101 ttttacttgt attgctcgtt gatttattga gtttatccat tgtgttgata ctggggttta
    11161 gtttagccct tgtaagttat tatcttgttt ctccgcaaat gtattttgga gaagagcata
    11221 tacaaatgac attggtgatc atatttacca ttattgcagg ttctacttta aattataaaa
    11281 cagccatgtt gcaacaacaa aaattggctg gcatggctgc tgctgcaggc atgattgccc
    11341 atgaattgcg ttcaccattg ttaggaataa agagcggcgc tcaagcttta gtccaatatt
    11401 tgccaagatt gattgatggt taccaattgg ctaaaaaaca agagttaata tcctcacctt
    11461 tacgtgacag attgttatta cagcttgggg gagtcagcaa tcgaattgtt agtgaaattg
    11521 attatgctaa tacgatcatt gatatgttat tagtcaaggc aagtcgcgat aatattttgc
    11581 aaaattgcat attagagact tgttctatag cagaatgttt gtctgaggct attaccagat
    11641 acccatttca gtcaccaaga gaaagaacat taatcacatg gactggtgat tttcaattta
    11701 aaggctctaa gttgttaatg cagcatattt tatttaattt aataaaaaat gcactttatg
    11761 ttatcgcaat ggcacagcgt ggggagatta atatttggac tgaatctggc gataagtatc
    11821 attatttgta ttttaaagac acagcaaaag ggatgagcaa acaacaatta aatcgattat
    11881 ttaatcattt ctatactact acctttatgg gaacaggcat tggtttatca ttttgtaaat
    11941 tagttatgaa tgcgtttggt ggtgatattg tttgtgaatc taaagagggc gaatacgctt
    12001 tgtttatact gagttttcca ggctcggttt aaaattggtt ttctgttgaa cataccatca
    12061 ttcgtcttaa aagacgtaag gatggtattg agttagagag ctcctgtgat tctcttctgc
    12121 tagagtttgg ccgtttttac tgagatagcg ataaaaatat atgtccaacc agtgacgatc
    12181 aattcaatcg cgataaaaag cccaatgatc cacagcccgc taactggcca ttgcattaga
    12241 attaatatcc caagaataat ggcggtgatt ccggccaata acaaccatcc ccatcctgtg
    12301 gaatctttta aggcaaaagc cataatcatt cttgttaaac caattacaat aagcaccgct
    12361 gccagaaaag ctgtgattag agtagatgct aaaaacgggt catacaaaac aatgcatccc
    12421 gctgcaatat acaacactgc aataagcgcc tgccatatca ttcctttcca ctctttgtat
    12481 ttaaatacgt ctataatgtg acttattccc ccaacaatta ataaggcacc gaaaaagaac
    12541 atacttacca aggttagacc aactaccatt ccaagaccaa cacagccaag gataaggaaa
    12601 aatattccaa atcctaaaat ccatccccag tttcgtttta actcatccac tgggggttgg
    12661 ttaaattctt gtgaattagc catatcaata tccttttttg atttcttagc taagat
//
LOCUS       DACTJE010000088        12669 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.088, whole genome shotgun sequence.
ACCESSION   DACTJE010000088 DACTJE010000000
VERSION     DACTJE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12669
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.088"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            230..1510
                     /locus_tag="I9760_001868"
     CDS             230..1510
                     /locus_tag="I9760_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5043633.1"
                     /translation="MNTLSFKHPLLAKTYLWIIWTIAACFFFYKYLIQVSPSVMTADL
                     MKAFQVNAAGLGNLSAFYFYSYLFMQIPVGVMLDRYSPRLLTTLAIFVCSISTYIFSQ
                     TNTLWLACLSRALMGAGAAFAAVSCFKLAAVWFSPKRFALVSGMFMTAAMLGAVGGQM
                     PLAFLVQHEGWRKALELVSVMGIILGVVYFLILRDKPAQTQPMPNSKEPVIQLLRRII
                     VNKQAWALSLYSGLAFAPVSVFGGLWGVPFLEKAYLLSRTDAALAISFIFVGFAAGAP
                     FWGWFSDFIRRRKPVLFTGTCSALLCLLVVIYSSNQNLLTLIILLFLFGFGASGFFTS
                     FAMIRELFPLVLVATVLGIMNTFNSVFEALFEPLVGALLDWTWEGTMVDGIHQFTLHG
                     YYFSLLLLPLSLILALLTLLLIDETYCRTKEEVE"
     gene            complement(1520..1939)
                     /locus_tag="I9760_001869"
     CDS             complement(1520..1939)
                     /locus_tag="I9760_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043634.1"
                     /translation="MCNWNRIIGLALMGFSAFLQAKTYIPQCPKEINTLERIQTSPDG
                     WETLSGIKNNYLGNVSFYSGHPKGQASLKPNRINKKKAEWKFSPHDTIYIVCHYNQTG
                     IELTQQLPQKTKGCTVIFNPNAKGPYGFLPQKITCNQ"
     gene            complement(1993..2259)
                     /locus_tag="I9760_001870"
     CDS             complement(1993..2259)
                     /locus_tag="I9760_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043635.1"
                     /translation="MESMIDLVGYVASSLVFATFYVKKILTLRLIAVCSNVAFITYGL
                     GYHLHPIFILHSVLLPLNLYRIFELLKNDPELTILARINLKATR"
     gene            2371..4092
                     /gene="legLC8"
                     /locus_tag="I9760_001871"
     CDS             2371..4092
                     /gene="legLC8"
                     /locus_tag="I9760_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegLC8"
                     /protein_id="HAT5043636.1"
                     /translation="MIFELNILPNRNFLFIFGDNMPLTSLKKFLKDIGKENIKQHQVK
                     LVLKTSQDIQLFLNALRDSRNHGISSLSELDLSDTRFTNQELSDLVTALNNIPGIKSL
                     RLDSCGLKDSDTVELSKLTHIKKLSLKSNYLKNRPMFNSMLEALYLDYNTELSASYVL
                     FSLSRNAAALKKLSLRNCGVTDANLEYLTRPESRLKSLTHFNLRRNNITHQGVDSFAH
                     LQSLTTIDLSQNTGIGDEGVSRLAPLKQLRTLYLDNCGIGGEGIKAIAKMNLQTVDLS
                     FNPGLKKEWGLDDIRPNHTIRTLLLTFCSLNDNHAKLIVSKFPAATDLNVANNNMTRA
                     GVKTLLSNPIIENLDVSTQSLYAKSTGASGTGMISPRIMMKKKEIKQQEKEKAQDLLD
                     TICNTITLKSINLEHTGITSRMLLSLIPDETDHKRYLKKINGVSCKELKPKLEQQIAL
                     RKAEKQSSPPVINAVEDSKTSDLMVKSGSSSKTEMQTREDDLIASPDEKIKALELENQ
                     KLKDELATARKRIFELENQSLSGSKTRKKTSIPPLAIPVMFQDFAKPGKNETDDNTIN
                     SITAIPK"
     gene            complement(4156..5121)
                     /locus_tag="I9760_001872"
     CDS             complement(4156..5121)
                     /locus_tag="I9760_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5043637.1"
                     /translation="MDLSKRIEPKTWSFVEKIQNAGGKPIYDIPVTEGRAIFDQLQAL
                     PTEKPDVDVEDKTLPVGPGGKVDIRIVRPKGAKEALPVIMFFHGAGWVFGDYQTHGRL
                     VREIAVGTHAAVVFVKYTLAPEAQYPTQIEESYAAMKYIAEHGKQFNLDTSRFVAAGD
                     SVGGNMTSVMTLLAKERGGPKIDYQVLIYPVTDANFDNASYKEFAEGPWLTKKAMEWF
                     WDKYLPNKEKRKEITASPLQASLEQLKGLPPALVITGECDVLRDEGEAYAHKLNAAGV
                     TVTAVRHLGTIHDFLMLNDLSETPACRNAVETIIGHLTHIFGKKK"
     gene            5350..6681
                     /locus_tag="I9760_001873"
     CDS             5350..6681
                     /locus_tag="I9760_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HAT5043638.1"
                     /translation="MLQFFEKGLQYSGEKISTDSYLMRKIVEAAIPSADKGTVSAEKM
                     LGLMNPNQIEIVSGSESAYRLIVHKIKEAKKQILIQAFVWEPGTQVVKDIRNALCDIK
                     HEVEVFLLVDQLDRLAQVFYHGEILPKKPKHDPACLGLDNLPQNIKLHIGTHVHNSIA
                     SNHNKAIWIDGDVILTGANFQQENYGPNGFHDAAMFIPGGAAEAVFYDFKAMWDKRTN
                     KSDEPEISPIYTGQESTESYRNTNSCSVLYVTNTIRQKWALLPFYRAPLPEDPLNNAY
                     MAAIKYAKHTIRIAVPNLNSPEIIHALADFINNRNGHVYLLMGKGFNDFRERFYGGTN
                     QSAIDYFSSLLDEDRQNNLQVRWFHRVNNNENSMKKEVIHMKFMSIDGQVVIYGSSNL
                     DLLSLHNSHETNIVIDNADFAQRAIEQLYLPIFETSIPVLISPKRTCFVCS"
     gene            complement(6863..7216)
                     /locus_tag="I9760_001874"
     CDS             complement(6863..7216)
                     /locus_tag="I9760_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043639.1"
                     /translation="MKKVILYALPSLMLAILFSPAYADEAPAQLTGTPAEQIQQLNAQ
                     IQSQLQEMQTKQQQQLDALNTQLQNQIKQVQSQLQEQIQTVNSQTQDQIKQIQATLQE
                     QIKQVQQQALQKANQ"
     gene            7373..7648
                     /locus_tag="I9760_001875"
     CDS             7373..7648
                     /locus_tag="I9760_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAT5043640.1"
                     /translation="MTKELCMRCYISGRVQGVWFRASAKNLAEQLMISGWARNLADGR
                     VEVFACGKEDKLEEFYTWLQKGPLNARVDVCTRENLPWEDYISFDVL"
     gene            complement(7785..8099)
                     /locus_tag="I9760_001876"
     CDS             complement(7785..8099)
                     /locus_tag="I9760_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043641.1"
                     /translation="MSTPKWNLSISRAVICVIALFASMICAAAHFVGGIHTNAGYVGW
                     YHPDYYNHDVIGVGNYHPGYGWVAPVYATPTYIIGNTGYNCQTVQQCDSEGNCIQSQN
                     CD"
     gene            complement(8419..9636)
                     /gene="legC2"
                     /locus_tag="I9760_001877"
     CDS             complement(8419..9636)
                     /gene="legC2"
                     /locus_tag="I9760_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC2/YlfB"
                     /protein_id="HAT5043642.1"
                     /translation="MTDTPKANLAVDEIKEDTPKDQPLPEENTEILVKKLKVLETTQE
                     NFDQIKKHIAAVIDSLAKNRSFFGRVAAYWGEMQLWLKITLGIVLVVPTLTLGIAIQV
                     ASLIVVSVLTLVTYVGSALLLENHVQKEEHITDRLKEEMIGLADNLGKVIESFEPLRK
                     QIAEQIEYFHKENDRLNLNVTQLSDHIMRLTKQAEQLQKTEQTLRKVREELEKTSETL
                     DEKVQVQTELLQINQEELNRIREEIAKNEIELSEKISELDQVKKEMSVEIEKAETVTQ
                     VLKNTVEKLSQTAIADHNHRESFQNKLNDFLSNKEQSFNDVAQRICEAEHELCFVKEE
                     LNFSNKRYQELLERQEEVVKRLEQLQFSRKPTPYSNAKVLGKLGIMATEEKESPTPVS
                     AEENTIPTQTLTG"
     gene            complement(9873..10400)
                     /locus_tag="I9760_001878"
     CDS             complement(9873..10400)
                     /locus_tag="I9760_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAT5043643.1"
                     /translation="MNRIKLGISNLIFMGRWLQLPLYLGLILILAGYVYRFIIELFEL
                     IFHLKGLDDTHIMLGVLDLIDVVMIANLLIMVVMGGYETFVSHLQLDSHPDQPEWLDH
                     FDAGAMKIKLALSLIGISSIHLLRTFIDPAKLSNYSIMWQVVIHLTLIVSALAIALTN
                     RLMDQPARKNLQHPD"
     gene            10635..11075
                     /locus_tag="I9760_001879"
     CDS             10635..11075
                     /locus_tag="I9760_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAT5043644.1"
                     /translation="MKYLHTMVRVSNLEQSLDFYCNKLGLIEVKRTENAKGRFTLVFL
                     AAPADIEQVQTSYGPMLELTYNWDPEDYTEGRNFGHLAYHVDDIYKICQHLQECGVVI
                     NRPPRDGHMAFIRSPDNISIELLQKGEPLPKQEPWLSAPNIGHW"
     gene            11167..12231
                     /locus_tag="I9760_001880"
     CDS             11167..12231
                     /locus_tag="I9760_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043645.1"
                     /translation="MQILKSLSEGMGTAGGVAWTTFGLLFGSFGLTVGNSALTISLGA
                     ISASLFLLVAIPVTYWSYCNSHKENDELRHAIEEQENQIVHYLTEYLLATRQECLLKN
                     PQKYRNHYDKSVLAEDLKNRLLKTRLKRPVSFTTAQFLDCLLDDNHKNSILGMLIDAC
                     TMDKLVLKKKLLTIYRGSVFLQFEELSLFAKLKTGFIAFCAGFGSLAGCSSGSLGLFA
                     ALGIFPGFAAVPLVGLAIMGIACIFGLLLSGMSIDTAIQRSKEKQLFLQSKAIHHDLK
                     NLATLKTASVKAELKAQTKYQIGAKEGLTEKLARPKLLKRSSSFDSYFYRNHPLWNIS
                     HNLPEASKCGFENLGSPFSR"
     gene            12559..12634
                     /locus_tag="I9760_001881"
     tRNA            12559..12634
                     /locus_tag="I9760_001881"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12592..12594,aa:Ala,seq:ggc)
BASE COUNT         3778 a         2181 c         2479 g         4231 t
ORIGIN      
        1 cttgctccca aagctgcaac agacagcctg ttggattgtc gcctgttgtt ttaaactctc
       61 tcatctcaat tgtcgtcgtg gtggtggtca gctttaccct ttcttctgct ctgagacggc
      121 ttctctcctg acttatctgc cccgcctcag ggcgataaca gcagcagata agacctggcg
      181 gtttttgaaa ttttatctct aacctgaagc aaataagtta ggagttattg tgaacacatt
      241 gtcattcaag catccattgc tggctaaaac ttatctttgg attatctgga ccatcgcggc
      301 atgttttttc ttttataaat atttgattca ggtttctccc agtgtcatga cagctgactt
      361 gatgaaggca tttcaggtta atgctgctgg ccttgggaat ttgtcagctt tttattttta
      421 ttcctattta tttatgcaga ttcctgtggg cgttatgtta gacaggtaca gcccaagatt
      481 gctaacaact cttgctattt ttgtctgcag tatcagtact tatatttttt ctcaaaccaa
      541 tactttatgg ttggcctgtt taagtcgagc attgatgggg gcgggagctg cttttgctgc
      601 cgtgtcttgt tttaagctag ccgctgtctg gttttctcca aaacgatttg ctctagtatc
      661 agggatgttt atgacagcag ctatgctggg tgctgtggga gggcagatgc ctttggcatt
      721 tttggttcaa catgaaggtt ggcgcaaggc gcttgaatta gtaagcgtga tggggattat
      781 cctgggggtc gtgtattttc tcattttaag agataagcct gcacaaacgc agcctatgcc
      841 aaatagcaag gaacctgtta ttcaattgtt aagacgtatt atcgttaata agcaggcctg
      901 ggctttgtct ttatatagtg gattagcatt tgctcctgtt tcggtttttg gtggattatg
      961 gggagttcct tttttggaaa aggcctatct cttatctcgt actgatgcag cccttgcgat
     1021 ttcatttatt tttgtcggtt ttgcggcagg cgcgccattt tggggatggt tttctgattt
     1081 tatcaggcgg agaaagcctg ttttgtttac aggaacgtgt tctgctctat tgtgtttatt
     1141 ggtagttatt tatagctcaa accaaaattt gcttacttta attattcttt tatttctctt
     1201 tggttttgga gcgagtggat tttttaccag ctttgcaatg atcagggaat tatttccttt
     1261 ggtacttgta gcgacagtac ttggtattat gaatacgttt aattcggtgt ttgaggcttt
     1321 gtttgagcca ctggttggtg ctttgctgga ttggacatgg gaggggacca tggtggatgg
     1381 tattcatcaa tttactttac acgggtacta tttttctctt ttattgttgc ctctgtcttt
     1441 aattttagcc ttattgacct tgttattgat tgatgaaaca tattgtcgaa caaaggagga
     1501 ggtagagtaa acctccccat tattgattgc aggttatttt ttgaggcaga aaaccatatg
     1561 ggccttttgc gtttggatta aaaatcacag tacatccttt tgttttttga ggcaattgtt
     1621 gtgtcagttc aattcctgtt tgattgtaat gacacacaat gtaaatggta tcatgaggag
     1681 aaaacttcca ttcggctttt ttcttattga tacggtttgg ttttaagctg gcttgacctt
     1741 tggggtggcc ggaataaaag gaaacattac ccagataatt gtttttaatc ccgctcaggg
     1801 tttcccatcc atccggagat gtctgtatac gttcaagcgt attaatttct ttagggcatt
     1861 gagggatata ggtttttgct tgtaaaaaag cagaaaatcc cattaatgcc aagccaatta
     1921 tcctgttcca attgcacacg gaagacccct tattgagtaa acagttatca tatcattaaa
     1981 tgatgaaaat gattatctgg ttgccttcaa attaatcctt gcaagtattg tgagctctgg
     2041 atcatttttt aataattcaa atattctgta taaatttaaa ggcaataaaa ccgaatgcaa
     2101 aataaatata ggatgtaaat gataacccaa accatatgta atgaacgcaa cattactaca
     2161 aactgctatg agtcttaatg tcagaatctt tttgacataa aatgttgcaa agactaaaga
     2221 agaagctaca tatccaacca aatcaatcat tgattccatt ttaacctcca cttccaaatc
     2281 cctttcattc aacataacac tgaccagagg agattagcaa gttttttaat caattcttat
     2341 ggttttctta agcgttgaaa tgtataatcg atgatctttg agttaaatat attaccaaac
     2401 aggaattttt tgtttatatt tggagacaat atgcccctta cttcgttaaa aaaattttta
     2461 aaagatattg gaaaggaaaa tataaaacag catcaggtaa aacttgtctt aaaaacatct
     2521 caggatattc aattattttt aaatgctttg cgagattcga gaaatcacgg aatctcgtcc
     2581 ttatctgaac ttgatctaag tgatactcgc tttactaatc aagagttgag tgatttagtc
     2641 actgctctta ataatatacc tggaattaaa tctttacgct tggattcttg tggtcttaag
     2701 gatagtgaca cagtggagct ttccaaatta actcacatca aaaaattatc attgaaaagt
     2761 aattacttaa agaaccgccc tatgtttaat tctatgcttg aagcattgta tttggattat
     2821 aatacagaac ttagcgcaag ttatgtgtta tttagtttaa gtcgcaatgc tgccgcttta
     2881 aaaaaattat ccttgcgtaa ctgtggcgtt actgatgcca atttagagta tttgactcgg
     2941 cctgagtcca ggttaaaaag tctgacacac tttaatttgc gaagaaataa tatcactcac
     3001 caaggagtgg atagctttgc tcatcttcaa tcgttgacta ctattgattt aagtcaaaat
     3061 actgggattg gtgatgaggg tgtatccagg cttgcgcctt taaagcagtt aagaaccctg
     3121 tatttggata attgtggaat agggggagag ggaattaagg caattgccaa aatgaatttg
     3181 cagactgttg atttgagttt caatcccggg ttaaaaaaag aatggggatt ggatgacatt
     3241 cgacctaatc ataccattcg aacattattg ctaacatttt gcagcttaaa tgataatcat
     3301 gccaagctta ttgtttcgaa gtttccagcg gctaccgatt taaatgttgc gaataataat
     3361 atgaccaggg caggggtaaa gactttatta agtaacccga ttatagaaaa cttggatgtc
     3421 agtacccaat cgctttatgc aaaatcaaca ggagcttctg gcactggcat gatctctccc
     3481 cgaataatga tgaagaaaaa ggaaataaaa caacaggaaa aagaaaaagc gcaggatttg
     3541 ctagatacta tctgtaatac aatcacatta aagagtatta atctggaaca tacaggcata
     3601 acctccagga tgttattaag tttaattcca gatgaaacag atcacaaacg gtatcttaag
     3661 aaaattaatg gtgtttcctg taaagagctt aagccaaaat tagaacaaca aattgccttg
     3721 agaaaagctg aaaaacaaag ttctcctcct gtaatcaatg cagtggaaga ttccaaaaca
     3781 agtgatttaa tggttaaatc tggcagtagt tccaagactg agatgcaaac cagggaagat
     3841 gacttgatag ccagtcctga tgaaaaaata aaagctctgg aactagagaa tcaaaaatta
     3901 aaggatgaat tggcaacagc tcgtaaaaga atttttgaat tggaaaatca gtctttatct
     3961 ggatctaaaa caagaaaaaa aacctcaata ccaccattgg caatcccggt gatgtttcag
     4021 gattttgcaa aaccaggaaa aaatgaaaca gatgacaaca ccatcaactc aataacggca
     4081 atccccaaat agcacaatta ttgtaaatgt attcagcatg actaacatcc gcttggatgt
     4141 tagtcatgta ttgttttact ttttcttgcc aaaaatgtga gttaagtgac caataatagt
     4201 ttcaacggca tttcggcatg ccggtgtttc agagagatca ttgagcatca ggaaatcatg
     4261 gattgtgccc agatggcgaa ctgcagtcac tgtcacaccc gctgcattga gtttatgggc
     4321 ataggcttcg ccctcatcac gcaaaacatc acactctcca gtgatgacta atgctggtgg
     4381 taggccttta agttgttcaa gagaagcttg tagcggactt gctgtaattt cctttctttt
     4441 ttctttattg ggcaaatact tatcccaaaa ccactccatc gcttttttgg ttagccaggg
     4501 gccttcagca aattctttat aggaagcgtt atcaaaattg gcatcagtta caggataaat
     4561 aagtacttga taatcaattt tggggccacc acgttcttta gcaagaagag tcatgacgct
     4621 ggtcatatta ccaccaacac tgtcacctgc agcgacaaat cgtgaagtat ccagattaaa
     4681 ttgcttacca tgttctgcta tatatttcat ggcagcgtaa gattcttcta tttgtgtcgg
     4741 gtactgagct tccggagcta atgtgtattt aacaaacacg actgcggcat gagtacccac
     4801 tgctatttct cgtaccagac gaccatgagt ttgataatcg ccaaaaaccc agccagcacc
     4861 atgaaaaaac atgatgacag gtaaggcttc ttttgcgcct ttagggcgaa caatgcgaat
     4921 gtcaacctta ccgccaggtc ctactggcaa agttttatcc tcaacatcga catcaggttt
     4981 ctcggtgggc aaggcttgta gttgatcaaa tattgcccta ccttctgtaa ccggtatgtc
     5041 ataaatcggt tttccacccg cattttgtat tttttcaaca aatgaccagg tttttggttc
     5101 tattcttttg cttaaatcca ttttgatttt ccttaattta taaataggga tgacttctta
     5161 tgtatagtat attgaattga aaaatcaagg aaatttgaat tcgtttgtgg ataataataa
     5221 caggtggatg gtgtctggga ttggatttac gatgaattaa attttgtttt gaatcgctca
     5281 aattaatttg caaaaatatt aaatcaattc aatgatataa attaggtaaa attaatttat
     5341 gagtgaatca tgctccaatt ttttgagaaa ggtttgcaat attcaggaga aaaaatttcc
     5401 accgattctt atttaatgag aaaaatagtt gaagcagcaa ttccctctgc tgataaggga
     5461 actgtttctg cagaaaaaat gctgggcctg atgaatccaa atcagataga aatagtatcg
     5521 ggtagtgaga gcgcatatcg tttaatagtt cataaaatca aagaggctaa aaagcaaata
     5581 cttattcagg cttttgtatg ggagccagga actcaggttg tcaaagatat ccgaaatgcg
     5641 ctttgtgaca tcaaacatga agtcgaagta tttcttctgg tcgatcagtt ggatcgtttg
     5701 gcacaagttt tttatcatgg cgaaatactt cctaaaaaac caaaacatga tccagcctgt
     5761 ttaggtttag ataatttacc tcagaatatt aaattgcata taggtactca tgttcataat
     5821 tccattgcgt ccaaccataa taaagccatc tggattgatg gggatgtcat tttgacaggg
     5881 gctaattttc agcaggaaaa ttatggtcct aatgggtttc atgatgccgc catgtttatt
     5941 cctggcggcg cagcggaagc ggttttttac gattttaaag caatgtggga taagcgcacc
     6001 aataaatctg atgaaccaga aatttctccc atatatacag gccaggagtc aacagaatct
     6061 tatagaaata caaactcttg ttctgtgctc tatgtgacaa atactatcag gcagaaatgg
     6121 gcgcttctcc ctttttatag ggcgccttta cctgaagatc cattgaataa cgcctacatg
     6181 gctgctatta aatatgccaa acatactatt cgaatcgctg ttcccaattt aaactcgccg
     6241 gaaatcatcc atgccctggc ggattttatt aataatagaa atggccatgt ctatctattg
     6301 atgggaaagg gattcaatga ttttcgcgag agattctatg gcggtacaaa tcagagtgcg
     6361 atagattatt ttagttcact acttgatgag gacaggcaaa ataacttgca agttcgttgg
     6421 tttcatcgtg tgaataacaa tgaaaattct atgaaaaaag aggttattca tatgaaattt
     6481 atgtcaattg atgggcaggt ggttatttac ggttcatcga atcttgattt gttaagctta
     6541 cataacagcc atgaaacaaa tattgtaatt gataatgctg attttgcaca aagagcaata
     6601 gaacaattat atctacccat ttttgaaacg agtatacctg tattgatatc gcctaaaaga
     6661 acctgttttg tttgttcttg attttaggct gttttgatca agccaatatt aattataatc
     6721 ctgatacatg agttcgacat aaaaaataat tccattttta tccaaactca tgcaaattac
     6781 catcaattgt ctttaagttt tagatgcata agcattgtca atgcccaagt ccacacgggc
     6841 gaagttatat aaattcaaat aactattggt tagccttttg caatgcttgc tgctgtactt
     6901 gtttaatctg ctcctgtagg gttgcttgta tttgtttaat ctgatcttgt gtttgagaat
     6961 tgaccgtttg aatttgttct tgtaattgag attggacttg tttaatttga ttttgtaatt
     7021 gggtatttaa agcatccagt tgttgctgtt gttttgtttg catttcctgt aattgagatt
     7081 gaatctgagc atttaattgc tgaatctgtt cagctggtgt gccggtcagt tgagcagggg
     7141 cttcatcagc ataggctggg gaaaacaaga ttgccaacat taaagatgga agtgcataca
     7201 atatcacttt tttcattttt tatctccatt attgaaaaaa tccaaattat ctaatttatt
     7261 ttgctctaat aagaatgcta atttaactat agcagaatca gagtgaaaac aggttatgca
     7321 aggtcaaatg aaatgaagta actgatattt ttacgatagg tggaaaagaa tcatgaccaa
     7381 ggagttatgt atgcgttgtt atatttcagg aagagttcaa ggagtatggt ttagagcttc
     7441 tgctaaaaat ttggctgaac aattgatgat aagtggatgg gctcggaatt tagcggatgg
     7501 tcgtgttgag gtttttgcat gtggaaaaga agataagctg gaagaatttt atacctggtt
     7561 gcaaaaagga cctcttaatg caagagttga tgtatgtaca cgagagaatt taccttggga
     7621 agattatata agttttgatg tgttataaaa atcaatgcgc tctattattg gttacctatt
     7681 aatctaatta acagttttga tgattagaga acctaagtgg agggaaattt gttctcaatc
     7741 tcgtaaaaaa gataatgcgc attgatatgc tacattttcc actactaatc acagttttgg
     7801 ctttgaatgc aatttccctc tgaatcacat tgctggactg tctggcagtt ataccccgta
     7861 ttgcctatta tatacgttgg agtagcatac actggagcaa cccagccata acctgggtga
     7921 taattaccca cgcctataac atcgtgattg taataatctg gatgatacca tcctacataa
     7981 cccgcatttg tatgaattcc accaacgaaa tgagctgcag cacaaatcat agaagcaaat
     8041 aatgcaatga cgcagataac tgcgcgagaa atggataaat tccatttggg tgtgctcata
     8101 gctctctcct tttcaaagaa tcatttcact ggtcacagtc aatattctaa aagtatagtt
     8161 cttttattgt tctctgtggg tttgtgttca taaagtaaaa aaattagctg atttaatata
     8221 aattgattat gggcaataga tctaaagtag ctcattaata gaacaattat gctgtctacg
     8281 aatagcttta gtttgagcct tatgctcagt atgtaattgg cgatctgttt taaaataaat
     8341 ttcgttgcac tgattggttt ttggttgacc gcacgtgata agcaatcgat gtgccatagc
     8401 acatcgattt aaaaacatct aacctgtgag agtttgagtt gggattgtat tttcttctgc
     8461 tgaaacaggt gttggagact ctttttcctc agtagccata atacctaatt taccaagtac
     8521 ttttgcatta ctatatggcg taggcttacg ggaaaattgg agttgttcca aacgtttaac
     8581 tacttcttct tgacgttcca atagttcctg ataacgtttg ttactaaagt ttaattcttc
     8641 tttaacaaag catagttcgt gctcggcttc acatatacgt tgagcaacat cattgaaact
     8701 ttgttcttta ttagagagaa aatcgttcaa tttattttga aacgattccc tatgattgtg
     8761 atctgcaata gcagtttggg acagtttttc tacagtattt tttaatactt gggtaacagt
     8821 ttcggctttt tcaatttcta ctgacatttc tttcttaact tgatccagct cagaaatttt
     8881 ttcagataat tcaatttcat tttttgcaat ttcttccctg attctgttta attcttcctg
     8941 atttatctgt agtaattcgg tttgaacttg tactttttca tccagagttt cagatgtttt
     9001 ttccaattct tctctgactt tacgcaaagt ttgttcggtt ttttgtaatt gttcagcttg
     9061 ttttgttaat cgcataatat gatcacttag ttgagtaacg ttgaggttta atctatcatt
     9121 ttctttatga aaatactcaa tttgttctgc aatttgtttg cggagaggct caaaagactc
     9181 gataactttt cccaggttat ctgccaaacc tatcatttct tcttttaaac gatcagtaat
     9241 atgctcttct ttttgaacat gattttcaag aagcagggca ctaccaacat aagtcaccag
     9301 agtcaggacg ctaactacta taagagaggc cacttgaatt gcaattccaa gtgtcaaggt
     9361 gggaacaacc aatactattc caagagtaat ttttaaccat aactgcattt ctccccagta
     9421 ggcggcaaca cggccaaaaa aactgcgatt ttttgccaag gaatctataa ccgcagcaat
     9481 atgttttttg atttgatcaa aattttcttg ggtagtttcc aatactttca gtttttttac
     9541 caatatttcc gtgttttctt cagggagggg ctggtctttg ggggtatcct ctttaatttc
     9601 atcaacggct agattagctt ttggagtgtc tgtcatgatt catattcctt tatttccatt
     9661 ttgactacat tcaacccgaa agagcttgca atctacttct ttctggttca atcccatgag
     9721 ttccctgaat aggtcaaact ttcctgattc tatcgaataa ttttaagcca gtcattattt
     9781 ttttagggcg ggactctgaa cataacaggt tttgtcatgt gcagagtgag tgacatcaga
     9841 gaaagaggtt atttaatttt cagattttta aatcaatcag ggtgttggag gtttttcctg
     9901 gcgggctgat ccatcaaccg attggttaat gcaatggcca aggctgaaac aattaaagtt
     9961 aaatgaatca caacctgcca cataatagaa taattgctaa gttttgcagg gtctataaaa
    10021 gttcttaaca gatgaatcga ggagatacct atcagtgaaa gagccaattt gattttcata
    10081 gctcctgcat caaaatggtc aagccattca ggttgatcag ggtgggaatc caattgcaaa
    10141 tgggatacaa aggtttcata gccgcccata actaccataa tcaaaagatt agcaatcatg
    10201 acgacgtcaa tgaggtccaa tactccaagc atgatatggg tatcatccag gcctttaaga
    10261 tgaaaaatga gttcaaataa ttcaatgatg aagcgataca catatccggc cagtatcaag
    10321 atcaaaccga gatacaaggg aagttgaagc catcttccca taaaaataag attactgatg
    10381 ccaagtttga ttcgattcat tttgtcgatt cactacaaaa tataacttat tgaatggtct
    10441 tatgataagc tttcctgaag gttttgttaa tacttattat actcatacta tgtataatat
    10501 gatcatattt aaatcattct taaatgttat actcttcaga gaattaacaa agcctatttg
    10561 ctaccggcat gatggttatt taaactaaat ttatgattaa taaaaaaata attttctata
    10621 gttaaggata attgatgaag tatttacata ccatggttag agtttcgaat ttagaacaat
    10681 cgctcgattt ttattgtaat aaattgggtt tgatagaagt aaaacgaact gaaaatgcca
    10741 aaggccgttt taccttggta tttttagctg ctcctgctga tatagagcaa gttcaaactt
    10801 cttatggacc aatgctagag ttgacttata actgggatcc ggaggattac actgagggaa
    10861 gaaattttgg ccatttggca tatcatgtgg atgatatcta taaaatctgc caacatttac
    10921 aggaatgtgg tgttgtaatt aataggccac ccagagatgg ccacatggct tttattcgtt
    10981 ctcctgataa tatttctatc gagttattgc agaagggcga gcctttgcca aagcaggaac
    11041 cttggttatc tgcacccaat attggacatt ggtaacctga caattgcatt ctggtagtga
    11101 atagtggaaa taatttaatc atactaaaat agtattttgg gccaaatggc aatgttgaat
    11161 caatcgatgc aaattttaaa atcactatca gaaggtatgg gaacagcagg gggggtggca
    11221 tggactacct ttggactgct atttggttct tttggactaa ctgttggcaa ttcggcactg
    11281 actatttcat tgggcgccat ttctgccagt ttattcctat tagtcgcaat acctgttact
    11341 tattggtctt attgtaattc gcataaagaa aatgatgaat tacgacatgc tattgaagaa
    11401 caggaaaatc aaatcgttca ttatttgacg gaatatttat tggcaaccag gcaagaatgt
    11461 ttacttaaaa atcctcaaaa atatcgtaat cattatgata agtctgtgct ggccgaagat
    11521 cttaaaaatc gattattaaa aaccaggtta aaacggcctg tttcgtttac gacagcacaa
    11581 tttcttgact gtctattaga tgataatcat aaaaactcta ttttgggtat gttaatagat
    11641 gcttgcacga tggataagtt ggtattgaaa aagaaactat taactattta tcgtggtagt
    11701 gtttttcttc aatttgaaga actttcctta tttgccaagt taaaaacggg atttattgcg
    11761 ttttgtgctg gatttgggtc gctggctggt tgttccagtg gttcattggg tttgtttgca
    11821 gccctgggta ttttccccgg ttttgctgca gttcctttgg ttgggttggc aattatgggt
    11881 atcgcttgta tttttggttt gttgttgtct ggaatgagta ttgataccgc aatccaaagg
    11941 agtaaagaaa aacagttatt tctccaaagc aaagcaatcc atcatgattt aaaaaatttg
    12001 gccacgttga aaactgcatc tgttaaggcc gagttaaaag cacaaaccaa atatcaaatt
    12061 ggagctaaag agggattaac agagaaatta gcaaggccta agctactgaa gcgatcttct
    12121 tcatttgata gttattttta tagaaaccat cccttatgga acatttcaca caaccttcca
    12181 gaagcttcga aatgcggctt tgaaaacctt ggttctcctt tttcacgttg aataacctgt
    12241 ccttttatta tattgctgcg ttgcaaactt tttctggtac tcagccttaa aacaaaaaac
    12301 caggttatgt aaagatctat tacaaccttt gttttcgtgc tggtcaaata tttttttgga
    12361 ttaggctctt acggtcaaaa atttttgctg caatggtgct tttacccatg ttcatttcta
    12421 ttgataaaat tagcgctgtg atttggattt atttatgatt ttgatgtaaa aaaaggttgt
    12481 cttataaaag acttgcatta aaatagaaaa accacttgac agtattaatg cgtctaatta
    12541 aactgcacac cttattttgg ggttatagct cagctgggag agcgcttgca tggcatgcaa
    12601 gaggtcggcg gttcgatccc gcctagctcc accatcaaca tcgttgtatg ttgatggtaa
    12661 aaaaagtac
//
LOCUS       DACTJE010000089        12569 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.089, whole genome shotgun sequence.
ACCESSION   DACTJE010000089 DACTJE010000000
VERSION     DACTJE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12569
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.089"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            2..916
                     /locus_tag="I9760_001882"
     CDS             2..916
                     /locus_tag="I9760_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5043646.1"
                     /translation="MHALEIKQLSKTYSNGVEALKGIDLTVNTGDFFALLGANGAGKS
                     TTIGLITSLVNKTSGNIKIYGFDLDAYPEKAKSCLGLVPQEVNLNIFENCEQILLNQA
                     GYYGISRKKAFPRAEALLEQLGLWDKRRSIVRHLSGGMKRRLMIARALIHQPKLLILD
                     EPTAGVDIEIRHSMWDFLARTNAEGTTIILTTHYLEEAEQLCKNIAIIDKGQIIKNTS
                     MKALLQTLRHQTFIFNTENPIDYLPDIHPFNVTPIDPTTFELRVDNNFSLNEVFSVLT
                     KQGIKIHSMRNKTNRLEELFMDLIKNGI"
     gene            906..1679
                     /locus_tag="I9760_001883"
     CDS             906..1679
                     /locus_tag="I9760_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT5043647.1"
                     /translation="MGFKYQLIALYTLVRREVVRMFRISSQVFLPPVITTTLYFLIFG
                     SLIGPRIGDIEGVDYSQFIAPGLIMMSVIINSYGNVSNSLFSVRFQKSIEEMLISPMH
                     NGLLLLGYVIGGVLRGLIVAILVFVISTFFLPVELDHLPMTLLVVVLVAAIFSLAGFT
                     NAMVARNFDDIMLIPTFILTPLTYLGGVFYSINMLPPFWQKISLFNPILYMVNALRHT
                     MIDQEEVSITLAMIIIFLMLIALTVGNMILLKKGVGFRE"
     gene            complement(1706..2725)
                     /gene="htpX"
                     /locus_tag="I9760_001884"
     CDS             complement(1706..2725)
                     /gene="htpX"
                     /locus_tag="I9760_001884"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HAT5043648.1"
                     /translation="MGIADYHATAGDWRAQLRRNEKKTRRVIAVFFLVYIFVGLIVDA
                     FVLQSMYPHASLENIIHALITFKVVPFATLLMIAFAGISLLITYALYDRIMLLGTEYY
                     QITPETARSLQEKQLYNVVEEMKVASGLQYMPKVYIIEANYMNAFASGYSEKSAMVAI
                     TRGLMEKLDRAEMQAVMAHELSHIRHHDIKLTLMVAVLSNILLIVIDILFYSVIYKRD
                     RRGDNRLLMVIIILRYVLPIITVLLALFLSRTREYMADAGCVELMRDNEPMARALLKI
                     NQDHVQNAEQYAKAYGDTPHEQVRQASYLFDPSHIDPVKSLNNAFSTHPSIEQRLEAL
                     GFKRK"
     gene            complement(2736..3317)
                     /locus_tag="I9760_001885"
     CDS             complement(2736..3317)
                     /locus_tag="I9760_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HAT5043649.1"
                     /translation="MKTFFIAILIIIALAFVWAISIYNTLIGLIEAINNNKKQIDIQL
                     DRRFKVFESLIEAVKKYMDYEKTTLKDVVALRNQAQAAKDAGDEQGRIAAENGISKIA
                     SNLNLVFEQYPDLKASQNVMQLQEEIVNTENKLAYAKQAYNDGIERYNAKKKSFFESM
                     VVAMFSSALDKEFIYWGLPEEQIQAKENYTVKL"
     gene            complement(3500..6355)
                     /gene="uvrA"
                     /locus_tag="I9760_001886"
     CDS             complement(3500..6355)
                     /gene="uvrA"
                     /locus_tag="I9760_001886"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAT5043650.1"
                     /translation="MHTITIRGARTHNLKNLDLELPRDKLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMEKPDVDSIEGLSPAISIEQKATSHNPRSTVGTIT
                     EIYDYLRLLYARVGEPRCPTHHTSLHAQTISQMVDQVLALPEDSKVMILAPVIRERKG
                     EHVQFLQQLQAQGYVRARIDGELYELDSPPKLGLRTKHTIEVVIDRFKVRPDLAQRLS
                     ESFENALNLADGLVIVSSMDGHFNELLFSSRFACSECGYSLSELEPRLFSFNNPVGAC
                     SSCDGLGVDQFFDPSRVVHDATASLAEGAIRGWDRKTTYYFSMLESLARHYGFDTSTP
                     FCDLSEAMQQIVLYGSGREIIEFQYHRPHGGYMVKRHSFEGIIPNMQRRYRESDSGMI
                     REELAKYLSSRPCQSCQGARLREEARHVFIDNKNLPEITSYSIEQAYQFFKELKLSGY
                     RGEIAAKINKEIVERLGFLVNVGLDYLSLARSAETLSGGEAQRIRLASQIGSGLVGVM
                     YILDEPSIGLHQRDNDRLLSTLMHLRNQGNTVIVVEHDEDAIRSADFVLDIGPGAGVH
                     GGEVVASGSPEEVMNNPASLTGQYLSGKKEIEIPKDRIPVNHDRMIHLKGVSCNNLQN
                     VDVSIPLGLLTCITGVSGSGKSSLINDTLYPIAANALNRASLMTIGEVKDISGLHLCD
                     KVIDIDQSPIGRTPRSNPATYTGLFTHIRDLFSGTPESRARGYQPGRFSFNVKGGRCE
                     ACQGDGLIKVEMHFLPDIYVSCDVCQGKRYNRETLEIHYKGKNIHEVLDMTVEDACAF
                     FAAIPVIARKCLTLMDVGLSYIKLGQSATTLSGGEAQRIKLARELSKRDTGNTLYILD
                     EPTTGLHFHDTKQLLTVLTRLREQGNTIVIIEHNLDVIKTADWIVDLGPEGGSKGGKI
                     IATGTPEMVAENPQSFTGQFLRQILSKSNELLLQKCK"
     gene            6451..6909
                     /locus_tag="I9760_001887"
     CDS             6451..6909
                     /locus_tag="I9760_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043651.1"
                     /translation="MKSLIKNLLFIVVFSINSSALYAINGNTRVIQWAQQTLMDTLSV
                     SYTSKPQDFVAVRKNYSLNAWSGITGFLGGNMQSIREKQLTLHPVLLPDTQILSTGLY
                     SGMSYWIIRQSVAIDELNTKLTFTLTILGRSGAAGSPYIVQSLSILLNAL"
     gene            7168..7719
                     /locus_tag="I9760_001888"
     CDS             7168..7719
                     /locus_tag="I9760_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HAT5043652.1"
                     /translation="MPKCMHFILATALIFVTSTFYASNLREIEQEVSDSVITAKITAK
                     FAESRHLNPLKISIDTEKGIVTLSGYAPDKQAFIDALQIAKNTKGVKAVHAKELHIKK
                     VNTAFTDAFITAEVETAVLKAKILDDESIPLVGINASTTNGEVTLSGHVKSARSIAAI
                     IKRVNEIPGVKKIVTNLEIQENT"
     gene            7716..8189
                     /locus_tag="I9760_001889"
     CDS             7716..8189
                     /locus_tag="I9760_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HAT5043653.1"
                     /translation="MSPLTQFKFFLWFFSHFKVALIGYLKPKLIQLTDNEIVVRLPLT
                     RRSRNHLHSMYFGALAVGADIAGGLHGFYHAKQAKCKVSLAFKSFQAQFLRRPESDVY
                     FICTEGDVVKKMIEESKKSGERINKPIHIKAYINYLTHPEEIADFILELSLKVIK"
     gene            8384..9097
                     /locus_tag="I9760_001890"
     CDS             8384..9097
                     /locus_tag="I9760_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase-C39 like family protein"
                     /protein_id="HAT5043654.1"
                     /translation="MIDLIIQTQPDDETCGPTSLHAIYLYYGLDLPLNEIIQGVDKSL
                     SGGTLTPMLGKHALLHGFRTTVYIYNLNIFDLTWFHNGEVNNSFLMDKLEAQMKYKND
                     PYITKASLAYLDYLKLGGTIRSKSLNVDLLKNYFNKKIPILTGLSATYLYRSAREYFT
                     PEGKSVYDDIRGEPCGHFVVLCGYDVKKRLVIIADPHAENPLSHNNYYKVSINRLINS
                     IMLGVLTYDANLLIIEPKE"
     gene            9101..10555
                     /locus_tag="I9760_001891"
     CDS             9101..10555
                     /locus_tag="I9760_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HAT5043655.1"
                     /translation="MQTVIVTDDLDSWSFLSELAPIVHALEYLSGDEYHQNKSFRVIN
                     LCRSYNYQSIGYYVSLLAQARDHKALPSVHSIQDVLNVSLSKLISQDTHEEIQHSLHD
                     IKGNEFVLSLYFGQNLAKRYATLAKQLHGLFPLPLIRFYLEKKKQWRIKTVVPLSLSD
                     VPEHHLEFMRQSAENYLSKKRVHQWRKKQRFHDLAILVDPSEPNAPSNKKALELFVSC
                     GEELGLNVDIIDKNEAKAIAEYDALFIRATTSVNHYTYRVSRRAAQENLVVIDDPQSI
                     IKCSNKVYLAELLSSHQIMTPDTLFISKYDKELPKIEFPCVLKKPDSAFSHGVVKIDD
                     EKSLQKSLNQFFKNSDLVVVQPFIPTEFDWRIGILDNKALFACRYFMAKGHWQIYDWH
                     CQEENKEGDSETLPLHEVPEQIIKIALKSTRLIGDGLYGVDIKSHGNKYYVIEVNDNP
                     NIDHGLEDKILGQNLYEQIMSVFLQRIRRKHGYV"
     gene            10548..11768
                     /locus_tag="I9760_001892"
     CDS             10548..11768
                     /locus_tag="I9760_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAT5043656.1"
                     /translation="MSDFPIFSVLGIEIEYMLVDKESLNVQPKSDLILRALAGHQVNE
                     IELGDIAISNELVLHVLELKNNGPKPLHVPVAEHFQKTIRELQPLLAQHNLLLLPTGA
                     HPWMDPNTETIRWPHGNHDIYNQFNSIFNCQGHGWANLQSMHVNLPYSNEEEFCQLHN
                     ATRLILPLLPAIAASTPFLDGQATGLLDSRLYFYSRNQQRIPSISGDIIPEFIRTERE
                     YQEQILEPMYRDISPYDPEGILQYQWLNSRAAIPKFDMKAIEIRILDSQECVEADIAI
                     AKAINAILKYWQSTSNFYLDNPCDTKSLKYVFDETIKNGLSCHIDNKRLLEQWQLPLQ
                     GMSVNTIWSLLIERISHELDRKQQLALELILKQGNLSERILKACSSCVNHDSLKQVYT
                     QLAHCLLRNEQFTP"
     gene            11749..12492
                     /locus_tag="I9760_001893"
     CDS             11749..12492
                     /locus_tag="I9760_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HAT5043657.1"
                     /translation="MNNLPPDIMKNIALLISCEHAVNTVPYEYQTLFEPHKDILESHR
                     GIDFGALEIAQSIHKNNPCSLFQATCTRLLVDCNRSINHPHCFSEITKNLSSDKKKKI
                     LDEFYLPFRNRVMDHIHQLISQGLQVWHLSTHSFTPVMNNIIRMTDIGLLYDPRSNSE
                     KILARQWQKEIKILHPQLKVRMNYPYKGISDGFTTSLRKKYSHHEYIGIELEVNQKLV
                     FHNHSLDALKNTLPLSVSNTIKAYQKSER"
BASE COUNT         4000 a         2588 c         2250 g         3731 t
ORIGIN      
        1 catgcatgcc ttagaaatta aacaactttc aaaaacctac tcgaatggag ttgaagcgtt
       61 aaaaggcatt gatttaacag tgaataccgg cgattttttt gccctgttag gagcaaatgg
      121 cgctggcaaa tcgactacca ttggtttaat cacatcttta gtcaataaaa cttcaggaaa
      181 catcaaaatt tacggcttcg acctggatgc ttatcccgaa aaagccaaat cttgtctggg
      241 acttgtccct caagaagtca atctgaatat ttttgaaaac tgcgagcaaa ttttactgaa
      301 tcaagctggc tattatggca tttcaagaaa aaaggctttc cccagagcgg aagccttgct
      361 agaacaacta ggactttggg acaagagacg ttctatagta cggcatttat caggtggaat
      421 gaagagaaga cttatgattg ccagagcact gattcatcaa cccaaattac tgattctgga
      481 tgaacccacc gccggtgttg atatagaaat aagacacagc atgtgggatt ttctagcgag
      541 aactaatgca gaaggaacaa cgattatcct gacgactcat tacctggaag aagcagaaca
      601 attatgtaaa aacatcgcaa taatcgataa aggtcaaatt attaaaaata cctcaatgaa
      661 agccttatta caaaccttac gccatcaaac ctttatcttt aacacagaaa accctattga
      721 ttatttgccg gatatacacc cgttcaatgt gactccgatt gatccaacta cttttgaatt
      781 gcgtgttgac aataacttca gtttaaatga agtattctct gtattaacaa aacagggaat
      841 aaagattcac agcatgcgca ataaaaccaa ccgattagaa gaacttttta tggatctgat
      901 taaaaatggg atttaaatat caacttattg ccctttatac cctggtaaga cgtgaagtgg
      961 ttcgtatgtt tcgcatttcg agtcaagtat ttttgccgcc agtaatcacc acaacgttat
     1021 attttctaat ttttggaagt ttaattggac ctcgtattgg agatattgag ggcgtggatt
     1081 attcacaatt catagcaccc ggattaatta tgatgtccgt tattattaat tcctatggaa
     1141 acgtctctaa ttcactattc agcgtaagat ttcaaaaaag tatagaggaa atgctgatta
     1201 gtcccatgca taatggactt ttattgttag gttatgtgat aggaggcgtg ctgagaggat
     1261 tgatcgtcgc aattctggtt tttgttatct ctaccttttt tctgcctgtt gaattagatc
     1321 atttaccaat gacccttcta gttgtcgtgc ttgttgcagc catattctca ctggctggat
     1381 ttaccaatgc catggtcgct cgaaattttg atgacatcat gctgataccc acctttatcc
     1441 tgacccctct tacttacttg ggaggagtct tttacagtat taatatgctg cccccctttt
     1501 ggcaaaaaat ctctcttttc aatccaattt tatacatggt aaacgcttta agacacacca
     1561 tgatcgatca ggaagaagtt agtattactc tggctatgat tattattttc ctgatgttaa
     1621 ttgctttgac agtagggaat atgattcttt taaaaaaagg ggtgggattt cgcgaataaa
     1681 aaacaggatt ccctgcaaaa cggatctatt tgcgtttaaa acccaatgct tccaggcgct
     1741 gttcaatact agggtgagtt gaaaaggcat tatttagcga ttttacaggg tcaatgtgag
     1801 aaggatcaaa aagataggaa gcttgcctta cctgttcatg aggggtatct ccataagctt
     1861 ttgcatattg ctccgcattc tgaacatggt cttgattaat tttaagcaaa gccctggcca
     1921 taggctcatt atctctcatt aattcgacac aaccggcgtc agccatgtat tctctggttc
     1981 ggcttaaaaa gagagctagt aaaacagtaa taataggtaa gacatatctt aagatgataa
     2041 taaccattaa caagcggtta tctcctcttc tgtctcgctt gtaaattacg gaataaaaca
     2101 ggatatcaat gacaattaat aaaatattac tgagcacggc aaccatgaga gtcaatttaa
     2161 tatcatgatg tctgatatgg cttaactcat gagccattac agcctgcatt tcagccctgt
     2221 ccagtttttc catcaatcca cgagtaatcg cgaccatcgc cgatttctca ctgtaaccac
     2281 tggcaaaagc attcatgtaa ttggcttcga taatgtaaac tttaggcatg tattgcaatc
     2341 ctgatgcgac tttcatctct tcaacaacat tgtacaattg tttttcttgt aagctacgag
     2401 ccgtttctgg cgttatttga taatactccg tccccaataa cataatgcgg tcataaagag
     2461 cataagtaat taacaaggaa atgcctgcaa atgcgatcat taataaagta gcaaaaggca
     2521 ccactttaaa agtgattaaa gcatggataa tattttctaa cgaggcatga ggatacatgc
     2581 tttgtaaaac aaacgcatcg actatcaatc ccacaaagat ataaactaaa aaaaagactg
     2641 caatcaccct tcgggtcttt ttctcatttc gcctcaattg agcacgccaa tctcctgctg
     2701 tggcatgata atctgcaatc cccatggtat attgctcaaa gtttcacagt gtaattttct
     2761 tttgcttgaa tttgctcttc tggaagcccc caatagataa attctttatc caaagcagaa
     2821 gaaaacattg ccacaaccat ggattcaaaa aatgattttt tcttggcgtt ataacgttct
     2881 atcccatcat tataagcttg tttcgcataa gctaacttgt tttctgtgtt aacaatttcc
     2941 tcttgtaact gcatgacatt ttggctagct ttcaaatcag gatactgttc aaaaaccaaa
     3001 ttcaaattgg atgcaatttt tgaaattcca ttttctgcag caattcgacc ttgctcatct
     3061 cccgcatctt tagcagcctg ggcctgatta cgtaaagcaa cgacatcctt taatgttgtt
     3121 ttttcgtaat ccatgtattt tttaactgct tcgatcagag attcaaaaac tttaaatcgc
     3181 ctatccaatt gaatatctat ttgttttttg ttgttattaa ttgcctctat caaaccgatt
     3241 agagtgttat agatgctaat tgcccaaacg aatgcgagtg cgataataat taaaattgcg
     3301 ataaaaaatg ttttcatctt cctatcctgt gaaaggttcc ttccctaatt tatagctcga
     3361 aatttcagaa aataacagct aagataagaa aaaagaaaca actggataac gtgttttttg
     3421 tcacaaaaag agattcaggt tgcctgaaaa acaacctgga tagcacatca agtataaaga
     3481 caaattgata attaatgaac tatttgcatt tctgtaatag aagctcattt gattttgata
     3541 aaatttgtct taaaaactga cctgtaaaag attgagggtt ttcagcaacc atttcgggtg
     3601 ttcctgttgc aataatcttt ccccctttac tgcctccttc aggaccaaga tcaacgatcc
     3661 aatcagccgt ttttatcaca tccaaattat gttcaataat gacaatggta tttccttgct
     3721 ctcgcaagcg agtcaacaca gttaataact gtttcgtgtc atgaaagtgt aatcctgttg
     3781 tgggctcatc gagaatatat agtgtatttc cggtatccct ttttgataat tctctggcca
     3841 acttgatcct ttgagcttct cctccagaga gagtagttgc actttgtcca agctttatat
     3901 aagacaaacc gacatccatt aatgttaggc attttctggc aataacagga atggcagcaa
     3961 aaaaagcgca cgcatcttcg acagtcatgt ccaacacttc atgaatattt ttgcctttgt
     4021 agtgaatttc caaagtttcc ctattgtatc ttttgccttg gcatacatcg caagaaacgt
     4081 atatatcagg taaaaaatgc atttcaacct tgataagacc atcaccttga caagcttcgc
     4141 accggccacc ttttacattg aaactaaagc gcccaggttg ataccctcgt gctcttgatt
     4201 ccggagtccc tgagaataaa tctctgatat gagtaaataa accagtataa gttgccggat
     4261 tggaacgcgg agttctgcca attggactct ggtcgatgtc aataaccttg tcacaaagat
     4321 gcagtccaga aatgtcctta acctcaccta tagtcattaa gctggctctg tttagtgcat
     4381 tggcagcaat aggatataaa gtatcattaa tcaaactgga tttacctgaa ccagaaacac
     4441 ctgtaataca agtcaacaaa cctaaaggaa tactgacatc aacattttgt aaattattgc
     4501 aagacacgcc ttttaaatga atcatccgat catggttaac cggaattcga tccttgggaa
     4561 tttctatttc ctttttccca gacaaatatt ggccggtgag agatgcggga ttgttcatta
     4621 cctcttcagg agaaccgctg gcaactactt ccccaccatg cactccagcc ccaggcccta
     4681 tatcaagaac aaaatcagcg cttcggatag catcttcatc atgttcgaca acgattacag
     4741 tgtttccctg gttgcgtaaa tgcattaaag tactcaataa tcggtcattg tcacgttggt
     4801 gcagccctat tgaaggctca tccaaaatat acatcacccc tacaagccct gaacctattt
     4861 gactagccag gcgaatacgt tgagcttctc cgccagatag agtctccgcg cttcgagcca
     4921 aagacaaata atctagcccg acattcacca gaaaccccag ccgctcaaca atttccttat
     4981 tgatttttgc tgcaatttca cccctatacc ccgataactt cagctcttta aaaaactgat
     5041 aggcttgctc aatagaatag ctggtgattt ctggtagatt tttattatca ataaatacat
     5101 gtcgagcttc ttcacgcaat ctcgctccct ggcaagattg gcaagggcgt gaggataggt
     5161 atttggcaag ttcctccctt atcataccag aatctgattc acgatagcgg cgttgcatgt
     5221 taggaattat tccctcaaaa ctatgccgct taaccatgta tcctccatgc ggcctatgat
     5281 actgaaactc gattatttca cgaccactac catacaaaac gatttgttgc attgcttcac
     5341 ttaaatcaca aaaaggcgta ctggtatcaa aaccatagtg cctcgcaagc gactcaagca
     5401 tgctgaaata atacgtggtt ttcctatccc atcccctgat tgccccttct gccaaactgg
     5461 cggttgcatc gtggactact cttgacggat caaaaaactg atccactcca agcccatcac
     5521 atgaggaaca tgcaccgaca ggattgttaa atgaaaaaag ccttggctca agttcactta
     5581 aactgtaacc gcattcagag catgcaaacc ttgaagaaaa taaaagttca ttgaaatgac
     5641 catccattga agaaacgatg acaagaccat ctgccagatt gagcgcattt tcaaaagatt
     5701 cactcaaacg ttgagccaaa tcagggcgta ctttaaagcg atcaataact acttcaatag
     5761 tgtgcttggt acgcaatcct aatttagggg gagaatccag ttcataaagc tctccatcaa
     5821 tacgtgcacg tacgtaccct tgcgcttgca attgttgcaa aaactggaca tgctctccct
     5881 tccgttcacg aataacaggc gccagaatca tcactttact gtcttctggt aaagccaata
     5941 cctgatcaac catttgacta atggtctgag catgcaagct ggtgtggtgg gtaggacacc
     6001 ttggctctcc gactcgggca taaagcaagc gcaaatagtc ataaatttcg gtaatggtgc
     6061 ctaccgtgga tcggggatta tgagaggtag ccttttgttc tatagaaatc gccggggaga
     6121 gcccctctat cgaatcaaca tcaggttttt ccatcatgga caaaaattgt cgagcataag
     6181 cagacaaaga ttccacgtaa cggcgctggc cttcggcata gagagtatca aaggctaaag
     6241 acgacttccc agaaccagac aatccggtta ttacgattaa tttatctcga ggaagctcca
     6301 ggtccaagtt cttgagatta tgggttcttg cgccacgaat ggtgatggta tgcataaaat
     6361 tcgctaattt gacttaaaaa atacatgagt atatctctgc tataataata aaacaaattg
     6421 cacactatga tgcgaaatag ggattttaat atgaaatcac taataaaaaa cttgcttttc
     6481 attgtggtat tttcaatcaa ctcatctgct ctctatgcta tcaatggtaa tacccgagtg
     6541 attcaatggg ctcaacaaac ccttatggat actttatctg tcagctatac ttcaaagcca
     6601 caagattttg tcgctgttcg caaaaattac tcacttaatg cctggagtgg aataaccgga
     6661 tttttagggg gtaatatgca aagcatacgc gaaaaacaac taacacttca tccggtttta
     6721 ctgcccgaca cccaaattct ctcaacaggt ctttattcgg gtatgagtta ttggattatc
     6781 agacaatccg tagccattga tgaactgaat accaaactga cttttacctt aaccatcctg
     6841 ggacgatctg gggctgcagg ctctccatac atagttcaaa gcttaagtat attgctcaat
     6901 gcattataat tacaaattaa acaggaatca cctgaaatat ggattgtctc catgaccaca
     6961 tggaatcatc aacatgaaag gtatactctc gctttaaatt ctgcttcaat ataaacagga
     7021 cttacgaaaa accccaaggt cgaggcaaaa aattttttta atgagagagt ttagatacac
     7081 taaatgacct aattaaaaac attttttaac aaagagattg gggtttttcg taagtcctga
     7141 taaaagcaac aactggagga cacaaccatg cctaagtgta tgcattttat tttggctact
     7201 gcattaattt ttgttacaag tacattttat gccagcaatc tacgggaaat agaacaagaa
     7261 gtaagtgatt cagtgattac tgccaaaatt actgcaaagt ttgccgaaag caggcacttg
     7321 aatccattaa aaatttcaat tgacacagaa aagggaatcg tgactctaag tggctatgct
     7381 cctgataaac aggctttcat agatgccttg cagatcgcca aaaacacgaa aggtgtcaag
     7441 gcggttcatg ccaaagagct ccacataaag aaagtcaata ccgctttcac agatgccttc
     7501 atcacggcag aagttgaaac tgctgttcta aaagccaaaa ttctggatga tgaatcaata
     7561 ccattggttg gaatcaatgc cagcacaacg aatggcgagg tcaccttgtc tgggcatgta
     7621 aaaagtgcgc gctccattgc tgccatcatt aaacgcgtaa atgagatacc tggcgtcaaa
     7681 aaaatcgtta ccaatttgga aattcaggaa aatacatgag tccattaacc caatttaaat
     7741 tctttttatg gttttttagc cactttaaag tagccttgat tggttattta aagcctaagt
     7801 taattcagct taccgataat gaaattgttg tgcgactgcc attgacaaga cgcagccgca
     7861 atcatctgca ttccatgtat tttggtgcct tggcagttgg tgcggatata gctggaggat
     7921 tacatggttt ttatcatgct aaacaagcga aatgtaaagt atccctggca tttaaatctt
     7981 ttcaagccca gtttctgcgt agacctgaat ccgatgttta ttttatatgc acagaagggg
     8041 atgtagttaa aaaaatgata gaagaatcaa aaaaatccgg tgaacgaatt aataaaccaa
     8101 ttcatatcaa agcttatatc aattatttga cccatccaga agaaattgct gattttatcc
     8161 ttgaactctc tttaaaagtt ataaaataac agcgttcgat tattatacaa atattaagcc
     8221 tacaaatgaa aattgttttt tcaatctcat tttcttaaat atcacatgat tatcagtctt
     8281 ctaaaatcac ttattattga attgattaca ccatttgaca aagaagccac tcatgtctaa
     8341 ttttaaatca tgatggcgct atttttaccg caaggatttg cctatgattg acctaataat
     8401 acaaactcaa cctgatgacg aaacttgcgg accaaccagc ttacacgcca tttatttata
     8461 ctacggtttg gatttgccat taaacgaaat cattcaagga gtggacaaat ctctttcagg
     8521 aggcacctta acacctatgt tgggaaaaca tgcccttctt catggattta gaacaactgt
     8581 ttacatctat aacctgaata tatttgacct cacctggttt cataatggtg aagtcaataa
     8641 ctcctttttg atggataaac ttgaagctca aatgaaatac aaaaatgacc cttatatcac
     8701 caaagcttct ctggcctatt tagactactt aaaactggga ggaacaattc gctccaaatc
     8761 actgaacgtg gacttgttaa aaaattattt taataagaaa atccccattt taactggctt
     8821 aagtgctact tatctctatc gcagtgcaag agaatacttt actccggaag gcaaatcagt
     8881 ttacgatgat attcgtggtg agccttgtgg gcattttgtt gtactgtgcg gttatgacgt
     8941 aaaaaaacgc cttgtaatta ttgctgaccc tcatgcagaa aacccactgt ctcataacaa
     9001 ttattataaa gtaagtatca atcgcttgat taactctata atgcttggtg tactaactta
     9061 tgatgcgaat ctactcataa ttgaacctaa agagtgataa atgcaaacag ttattgttac
     9121 tgatgatctg gatagctggt cttttttaag tgagcttgcc ccgatagtac atgccttgga
     9181 atatcttagt ggggatgagt atcatcaaaa taaatcattt cgggtcatta acttatgccg
     9241 atcttataat taccaatcga ttggctatta tgtatcttta cttgcacaag cccgtgatca
     9301 caaagcatta ccctctgtac acagtattca ggatgtttta aacgtcagtt tatccaaact
     9361 gatctctcag gatactcatg aagaaataca acacagtctc cacgatatca aaggcaatga
     9421 atttgtttta agcctctatt ttggccaaaa tctggcaaag cgatatgcaa cattggcaaa
     9481 acaactccat ggtctttttc ctttaccact cattcgcttt tatctggaaa agaaaaagca
     9541 atggcgaatt aaaacggtag ttcctttgtc tttgtcggat gtaccagagc atcatctgga
     9601 gtttatgcgc caatcagctg aaaattattt atcaaaaaag agagttcatc aatggcgtaa
     9661 aaaacaacga tttcatgatt tggccattct tgtcgacccc tccgaaccca atgccccatc
     9721 caataaaaaa gcccttgaac tttttgtttc ttgtggtgag gaactgggat taaacgttga
     9781 catcatagat aaaaatgaag caaaagcaat cgctgaatac gacgctcttt ttatcagagc
     9841 aactacttcc gttaatcatt atacttatcg cgtgtcacga cgcgcagccc aggagaattt
     9901 ggtcgttatt gatgacccac aatctataat taaatgctcc aacaaagttt atcttgcaga
     9961 gctcttaagc agtcatcaaa tcatgacgcc agacacctta ttcatcagca aatatgataa
    10021 ggaattaccc aagattgaat tcccctgtgt tttaaaaaaa cctgacagcg ccttttctca
    10081 tggcgtggtt aaaatagatg atgaaaaatc tttacaaaaa tctcttaacc agttttttaa
    10141 aaattctgat ttggtagtgg ttcaaccgtt tataccgact gaatttgatt ggcgcatcgg
    10201 catccttgat aataaagcac tttttgcctg ccgctatttt atggcaaaag gtcactggca
    10261 aatctatgat tggcattgcc aggaagaaaa taaagagggg gatagtgaaa cactacccct
    10321 tcatgaagtg ccagagcaga ttattaaaat agctctgaaa agtacacgtt tgattggtga
    10381 cggattatat ggtgtcgata taaaaagtca tggcaataaa tactacgtta tagaagtgaa
    10441 cgataatccc aatattgatc atggccttga agataaaata ctaggccaaa atctgtatga
    10501 gcagatcatg agtgtcttct tacaacgaat tcgcaggaaa catggttatg tctgattttc
    10561 ctattttttc tgtcttgggg attgaaatag aatatatgct ggtggataaa gagagtttaa
    10621 atgtccaacc taaaagcgat cttattctaa gggctttggc aggtcatcag gttaatgaaa
    10681 tagaattagg agacattgcg ataagcaatg aattagtatt gcatgtcctt gagttaaaaa
    10741 ataatggtcc caaaccacta catgttccag tagctgaaca tttccagaaa accattagag
    10801 agctccagcc ccttcttgct caacacaatt tattgctatt acctactggc gctcatccct
    10861 ggatggaccc aaatactgaa acaattcgct ggcctcatgg taaccatgat atctataatc
    10921 aatttaattc aattttcaat tgccagggac atggctgggc aaacttgcaa agtatgcatg
    10981 tcaacctgcc ttattccaat gaagaagaat tttgtcaatt gcataacgcc acacgcttga
    11041 ttttaccttt attacccgcc attgcggcaa gtactccatt ccttgatggc caagctacag
    11101 gattactgga ttctcgcttg tacttttata gtcgcaatca gcaacgcatt ccctccatca
    11161 gcggcgatat tattcctgag tttattagaa cagaaaggga ataccaggaa caaattctcg
    11221 aacccatgta tcgagacatt agcccttatg atcccgaagg tatattacaa tatcaatggc
    11281 taaattctcg tgctgcaatt cccaagtttg acatgaaggc tattgaaatt cgcattcttg
    11341 acagccagga atgtgtagaa gcagacattg ccatagcaaa agcaatcaat gccattttaa
    11401 aatactggca aagcacctcg aatttttatc ttgataatcc atgcgacacc aagagtttaa
    11461 aatacgtttt tgatgaaacc ataaaaaacg gattaagttg tcatatcgat aacaaacgat
    11521 tactggagca atggcaatta ccccttcaag ggatgtcagt caataccatc tggtcactat
    11581 tgatcgaaag aattagtcat gaactggata ggaaacagca actggctctt gaacttattt
    11641 taaaacaggg aaatttgagt gaaagaatcc tcaaggcttg tagctcctgt gtcaatcatg
    11701 attctttaaa acaagtgtat acgcaattag cccattgttt gcttcgtaat gaacaattta
    11761 ccccctgata taatgaaaaa tatagccctt ctaatcagtt gtgaacatgc tgtcaataca
    11821 gttccctatg aatatcaaac cctgtttgaa cctcataaag acatattaga atctcataga
    11881 ggcattgatt ttggtgcgct agaaattgca caatcgattc ataaaaacaa cccttgcagt
    11941 ttgtttcaag ccacctgtac aaggctcttg gttgattgca ataggagtat caatcatccc
    12001 cattgttttt ctgagattac aaaaaattta tcatcggaca aaaagaaaaa aatactggat
    12061 gaattctatt tgccgtttcg caatcgcgtc atggaccaca ttcaccaact gattagccaa
    12121 ggactgcaag tttggcatct gtccacacac agttttactc ctgtaatgaa caacatcatt
    12181 cgcatgactg acattggcct tctatatgac cctcggagta attcagaaaa aatcttggcc
    12241 agacaatggc aaaaagaaat caaaatactc cacccccaat taaaagtacg tatgaattat
    12301 ccttataaag gaatcagtga tggctttact acatccttgc gtaaaaaata cagtcatcat
    12361 gaatacatag gcattgaact ggaggtcaat caaaaacttg tctttcacaa ccacagcctg
    12421 gacgcactca aaaatacact gccattgagc gtatcaaaca cgattaaggc ttatcaaaaa
    12481 tcagagaggt aagcgacaac atttttttca agttgcaatt taaaaaaacc aagtggctga
    12541 attaaaaaat ttttttacaa aaaagattg
//
LOCUS       DACTJE010000090        12485 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.090, whole genome shotgun sequence.
ACCESSION   DACTJE010000090 DACTJE010000000
VERSION     DACTJE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12485
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.090"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            4..978
                     /locus_tag="I9760_001894"
     CDS             4..978
                     /locus_tag="I9760_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HAT5043658.1"
                     /translation="MRYRRFLCLLNGMLLSSNVAAFSPSAPIFSPYVDLTINTHWDSQ
                     SQDMEPMDLISPAKKLGIKAYHLAFITDSGQCQPAWGAQQNYSVAKGWGKKQFDTLSR
                     EGVKLTVSFGGASGTDISYHCDKNQLINTFSQVANQYHANVLDFDIENGTANIPNLLQ
                     ALKLFQKEHPDVLLSFTLPVMPEGLTSVGKEIITSAATLGLHFNVNIMAMDYGPAYSG
                     DMGDYAISAATNLHQFLQEIYPDKKPEALWQMIEVTPMIGVNDVNTEQFTLSNAAQLK
                     QFAQKNLLGGLSMWSFNRDKPCADKWASPVCSGNNLQSHDYEFVKYFQ"
     gene            1228..3000
                     /gene="lem20"
                     /locus_tag="I9760_001895"
     CDS             1228..3000
                     /gene="lem20"
                     /locus_tag="I9760_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem20"
                     /protein_id="HAT5043659.1"
                     /translation="MKSKEERFNEWRQEKAKSEQAYIKTVPLVEEYIKSFDTLAELRN
                     DVANPNSEKIKKMDAHKRRTLGVLLYAKLKLDEVIEKISDSDSQEKDLLIELNNTLSK
                     AYDNPKSDAKNRVKILIETTEHLLNIEHHSYPQLAKELCADLYALLCIAKRSLKKNEI
                     LASWFIDVHEINKKLEELLAKTEEKVESLDKILSEKLNESLETSPKDRTIQEHFNERF
                     TEILSGDEKQRTKLDKLKMSLKEVAEGLSGIVELRKEKATIGEKIKKIEALLKTVEEN
                     DKKVTGRQYFLDLLASHKDHFNTLMSTEGGELKKQLAGKIEQLKNPDFYQTVSSTLLW
                     GASWATSLGTVIYRQITPQAVQDRVSAYAPATLDSEAKALLQELARQQLIELKKQQSL
                     KRDELVQRYSQLSSGSPELEKLIINATSDHLEQIAAATTETSKVLDGYDQIEIKLKDN
                     VEKLKGNQLTRKQLSDFIEKNDTWIVKLSNWLAENIHEIFKSDTARMIDKARKAQQKL
                     GEFERQYQTEIESEKAKIENNDSLSSEMKVFLKNQFEETKKTGGMEQKPQKNLSKETL
                     QKLFVSLLPEHEEISSGVIFSPFN"
     gene            3021..4616
                     /locus_tag="I9760_001896"
     CDS             3021..4616
                     /locus_tag="I9760_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HAT5043660.1"
                     /translation="MSQSVDQSQVIDRLNRYLKWHNMPVSMNEEGICNGLVTVYAKYV
                     LDGKEKRFFELLDQIVRMEADSAIESEINQFVYDVVLTLFPHQFNKKLSQANSMCALT
                     VNNKPLVSSFDFAIATQDKNWEEIFKNLALQEDEIARVSGTAHAVAVRRKNNKYIVYD
                     PNYSSGTKEFSNEHELISELHYNVFGYHNGFYIKGGLLGMMVSVIRYPDNKKPRAFPQ
                     VTQLYDRYLTKNNINDVAVSFHGGTFNTLEHAAMVSHAEVIKNLLEKGAKDNDHQAAG
                     NAVMYNNVEALKALLGKNRETELFQRLFHAALESGREEVYDALFSMKGALPFNSSVQA
                     IRAAAKGGNSVLLAKVMNYFKRNHTGFSDLEDAIFSAITSGSVQCVKLLVNQLNESKK
                     PLDDKKRMEYLLESIKQNQSYMVAYFLKDIPPEHLKTIYMSVSAVERTNLHILRQLQN
                     YGVPFSDMSKAVFEQKEHHFVGLKRILGIFLCKFTDFLKELLFNDRGIHCHRAEESGV
                     KKSFFAFPKKLVGVEQLQIPKLY"
     gene            4695..5618
                     /locus_tag="I9760_001897"
     CDS             4695..5618
                     /locus_tag="I9760_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAT5043661.1"
                     /translation="MNILLAGGSGLIGQELVRGMGEEHHYTVLGRDKDAIQKIFTTKA
                     SSLSWDELNTQDASRFDAVINLCGVNIAISRWTPKIKEEIISSRVQTTENLLHWILQQ
                     NAKPRFYCANAVGIYGLQENGDATDLTEDTAINWEHPKDYLSEVGIRWQQALQKAEEA
                     GLPVTSTRFGVVLKKGKGFLGKLYPSFICGLGSTIGDGRQYLSWIDSEDLVNAYRFLL
                     NHPEITGPVNVTSPNPCMQREFAKAFAKSLHRPLLLWTPAFIIHLLFGEMGDCLINHG
                     QKVKPKRLLEAGFEFKWPDIEKALKHQYSAI"
     gene            complement(5715..6131)
                     /locus_tag="I9760_001898"
     CDS             complement(5715..6131)
                     /locus_tag="I9760_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4949 domain-containing protein"
                     /protein_id="HAT5043662.1"
                     /translation="MMFQSKLATFFGALILAGSAFASQQGACPDINVIKAEGLPMAEE
                     IGMNYYLAYNISDYSTSSVWGFFIAPIEADSEEGALEASNEVLSMMNAPGVPHQQDED
                     IICSYDTGTPNLIAAAIKDGSQISPMKLKQYFKSAR"
     gene            complement(6408..7682)
                     /locus_tag="I9760_001899"
     CDS             complement(6408..7682)
                     /locus_tag="I9760_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylpolyamine aminohydrolase"
                     /protein_id="HAT5043663.1"
                     /translation="MAKDKGFFKKAKTGKNKSSATPYAEECAIQIPSEIDNEQMHRMP
                     AGGEEDQYLRIKHMSALIKKYGNLPIITTRETRLPDYWLELFTAIDEGDTPKAHALFH
                     LLPQEDIILRALRAVHSEDYLYQLIKYCIQAKHFGFKQLNADLVVTPKTFEILIKDCA
                     TTLFNPAKTHFSFGLPSHHAYTQMGSGFCLINKTAMLIKQAELSSVQPPRFVIIGTDV
                     NRDNGLCDILRHSFSHLSICHIDVFDSRVYPQQDFSYINNEFNSEGVDVGKNIHVWHH
                     NDLSYYAVDLSLTSRKSVGVHPALLFALEQFKASIREAKVKGQKIALYLPTGWDSHED
                     ETAYCGKFVKGRMLGKTAAHQFRFNDGDLSYFYESIFTLYNENKDCIDTIYWGLEGGY
                     DRTMYKRELTLLLQVIEKQLLPQDSNSHNMSC"
     gene            7882..8286
                     /locus_tag="I9760_001900"
     CDS             7882..8286
                     /locus_tag="I9760_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAT5043664.1"
                     /translation="MVQHDIIKLPPKPYGAHLITNEVMQKITLRGTGLLHLFIQHTSA
                     ALALNENASPDVLIDLNHFFTQLVPEHQHYRHDIEGPDDMPAHIKSILCGSSLTIPFQ
                     ENKLLLGVWQGIYLMEFRKQAGARTIVASQLI"
     gene            8420..9469
                     /locus_tag="I9760_001901"
     CDS             8420..9469
                     /locus_tag="I9760_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HAT5043665.1"
                     /translation="MTNDHYVSKKKMFAVVFCVFAPNEVLLANDTAVGGEGSLPIPVS
                     QPHIKMVSELIRISGKNLNEPQMNGSWHYNCNFTFKNTLNKEVTISMAFPFPINDGNS
                     EIALPAGQQTNVGQALVYDFLVTVNDKQVSAQRGNIAPDQNKGLYYEDAYFWKTTFPP
                     LATVNIHHDYSTGATYDVMGYHWVRYVLKTGALWQDSSIGHTRLEVIPNTPTRLCSEI
                     DQKADYLNPTPSGMSISGSRADRKYIWDLRHFQPQADLSLCLFTGISYVRYKVIYPWL
                     NSDDALSKLARLSNKELRFLRNTIYAQYGRQFQSPDLQEYFSKKWWYVPNPDYSDRML
                     NEEDKKLLSMINQAK"
     gene            complement(9630..10157)
                     /locus_tag="I9760_001902"
     CDS             complement(9630..10157)
                     /locus_tag="I9760_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HAT5043666.1"
                     /translation="MAIFKNLVLTLVSSALLVTPCFSAGLTLESPAFASNAAIPQKFT
                     CSNLNYSPPLIWHDPNLGTQSYVLIVHDPDAPTGNWIHWVLFNIPAQVKQLTEGTTTP
                     AGATSGLNSWNTTGYSGPCPPSGTHRYYFTLYALDTYLSLGPEATAQDVVNAMKGDII
                     DTAELVGHYSKNASQ"
     gene            10477..11490
                     /locus_tag="I9760_001903"
     CDS             10477..11490
                     /locus_tag="I9760_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAT5043667.1"
                     /translation="MKAIGYYHSLPITDNKALMDLDVSVPKATGHDILVEIKAVAVNP
                     VDTKVRRNVQPEPHQPKILGWDASGVVTEIGSEVTMFKPGDEVWYAGDLTRPGSNCEF
                     HLVDERIAAKKPKSLSFQEAAALPLTSLTAWELLFDRLQLSAEDKGILLITGAAGGVG
                     SILVQLARQLTQLTIIGTGARPESIQWILDNGAHHVIDHTKDMKAQLQNLHYSEVDYV
                     ISLTHTDQHAKSLVEVLKPQGKFALIDDPQNLDIKLFKLKSISIHWEMMYTRSMFKTA
                     DMIKQHDILTEVARLVDAGKIKTTIAECLGLINADNLRRAHALLESGTSRGKIVLNGF
                     KSI"
     gene            11833..>12485
                     /locus_tag="I9760_001904"
     CDS             11833..>12485
                     /locus_tag="I9760_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HAT5043668.1"
                     /translation="MIIYLLWLLAVTSFNFSTSTIPVSKAEPQGNLLYSEIPSIKMPL
                     NEIKTLLQKEGNSLQPAVIDKVITTIQCANAYQVDRNNILTIIDYSMPSNQKRLWVFD
                     LDKKELLFHTYVSHGIKSGTLLTDKFSNKFDSKASSIGVYKTEQVYYGREGLSLRLVG
                     LDTKFNDNAFNRYIVMHGGWYMDEQFIKRYGRPGRSWGCPALPLPIKKQIIDTIKDNS
                     "
BASE COUNT         4013 a         2125 c         2531 g         3816 t
ORIGIN      
        1 tgaatgcgat atagacggtt tttatgttta ttgaatggca tgttattatc cagtaatgtt
       61 gcagcgtttt caccctctgc ccctatattc agcccttatg ttgatttaac gattaatact
      121 cattgggact cgcaatcaca ggatatggag ccaatggatt tgatttctcc ggcaaaaaaa
      181 ctggggatta aagcttatca tttggctttt attacagaca gtggtcaatg ccaacctgcc
      241 tggggagccc agcaaaatta cagtgtggca aagggatggg gtaaaaaaca gttcgatact
      301 ctatcaagag agggggtgaa actcaccgtt tcatttggcg gagcgagtgg tactgatatt
      361 tcctatcatt gtgataaaaa tcaattaatt aatacgttta gtcaagtggc taatcaatat
      421 catgcgaatg ttctggattt tgatatagaa aatggaacag ccaatattcc taatttgttg
      481 caggctttga aattattcca aaaggagcat ccggatgttt tgttaagttt tacccttcct
      541 gtgatgcctg agggtttaac ttctgttgga aaagagataa tcacctcagc ggctacttta
      601 ggccttcatt ttaatgtgaa cataatggct atggattatg ggccggctta tagtggagat
      661 atgggggatt atgcgatatc cgcagcaacg aatctccatc aatttctcca ggaaatttat
      721 ccggataaaa aaccggaggc tttatggcag atgattgaag taacgccaat gataggtgtg
      781 aatgatgtga atacagagca atttacctta tctaatgcag ctcaattaaa acaatttgca
      841 cagaaaaacc tgctgggggg attgtcaatg tggtctttca atcgggataa accttgtgct
      901 gacaaatggg caagtcctgt atgtagcggt aataatttgc agagtcatga ttatgaattt
      961 gtgaaatatt ttcagtaatg gaattaattc aggatttcct ggttctgatg aagaagaaat
     1021 ggtttgaatg aattatctat gttatttttt tgtcaagata aacctggttt tgatgaatta
     1081 ggtgatataa ttttcactat gataaattct acgaaattca ttacttaata tttagataat
     1141 aatagatgca tatactggag ttaagattta gcgggtattt ctccatttat tgtttggaag
     1201 agtgctaact ggcaattagg gttaattatg aagagtaaag aagaacgatt taatgaatgg
     1261 cgccaagaaa aggcgaaatc agaacaggca tatattaaaa ctgttccatt agtggaagaa
     1321 tacattaaat catttgatac cttggctgag ctcagaaatg atgtggctaa tcctaattct
     1381 gaaaaaatta aaaaaatgga cgcccataaa aggcgcactc tgggtgtttt actctatgcc
     1441 aaattaaaac tggatgaagt gatagaaaaa atttcggatt ctgatagcca ggaaaaggat
     1501 ttattaattg agttaaataa tactctgagc aaagcgtatg ataatcccaa atcggacgcc
     1561 aaaaacagag taaaaatttt aatagaaacg acagaacact tattgaatat cgaacatcac
     1621 tcttatccac aattggctaa agaattatgc gcagatctat atgctctatt atgtattgcg
     1681 aagagatcgc ttaagaaaaa tgaaatactg gcaagctggt tcatcgatgt tcatgaaatt
     1741 aataaaaaac tggaagaatt attagcaaaa acagaagaaa aagtcgaaag tttggacaaa
     1801 atcctgtcag aaaaactcaa tgaatcactt gagacttccc caaaagacag aacgatacaa
     1861 gaacatttta atgagcgttt caccgaaatt ttaagtgggg acgagaaaca aagaacaaaa
     1921 ctggataagt taaaaatgag ccttaaggaa gtagctgagg gactatcagg tattgtggag
     1981 ttaagaaaag agaaggctac gattggtgaa aaaattaaaa aaattgaagc tttactgaaa
     2041 acagtagaag aaaacgataa aaaagttaca ggaagacaat attttttaga cttactagcc
     2101 agccataaag accattttaa tactctaatg agtactgagg gtggggagtt aaaaaaacag
     2161 ttagcaggga aaatagaaca actcaagaac ccggattttt atcaaactgt ttcttcgacc
     2221 cttttatggg gcgcaagctg ggctacttct ttgggtacag tcatttatcg ccagataact
     2281 cctcaagcag ttcaggatag agttagcgct tatgctcctg caacactgga tagtgaagca
     2341 aaagctcttt tgcaggaatt ggcaagacaa cagttaattg agttaaaaaa acaacaaagt
     2401 ctcaaaaggg atgagttggt tcagagatat agccaactat caagcggtag ccctgagttg
     2461 gaaaaattga taatcaacgc cacttcagac catcttgagc aaattgctgc agcaacaaca
     2521 gaaacaagca aagttcttga tggatatgat caaattgaga ttaaactcaa agataatgta
     2581 gaaaaactta agggaaatca gttaactcga aagcaattat ctgattttat tgaaaaaaat
     2641 gacacctgga tagtaaaatt atccaactgg cttgcagaaa atatccatga gatatttaag
     2701 tctgatacgg ctcgcatgat tgataaggct agaaaagcac aacaaaagct gggagaattt
     2761 gaaagacaat atcaaacgga aattgaaagt gaaaaagcaa aaattgaaaa taatgattca
     2821 ttgtcctcag agatgaaagt ttttttaaaa aatcagtttg aagaaacaaa aaaaacaggt
     2881 ggaatggagc agaaaccaca gaaaaatctc agcaaggaaa ctcttcagaa attatttgtt
     2941 agcctgttgc ctgaacatga agagatctca tctggggtaa tattcagtcc gtttaattaa
     3001 tcagggatta gagcgatgat atgagtcaat cggttgatca aagtcaagtc attgatcgat
     3061 taaatcggta tctcaaatgg cataatatgc cagttagcat gaatgaagaa ggaatttgca
     3121 atggcttggt taccgtgtac gcaaaatacg tactcgatgg taaagaaaag cgcttttttg
     3181 aattgcttga tcagatagtc agaatggagg cagactcagc aatagagtct gagataaatc
     3241 agtttgtcta tgatgtcgtt ctcactttat tcccgcatca atttaataaa aaactaagtc
     3301 aggctaattc catgtgtgcc ttgacagtaa acaataaacc cttggtttct tcttttgatt
     3361 ttgcaattgc aactcaagat aaaaattggg aggaaatctt taaaaatctt gctctgcaag
     3421 aggatgagat tgctcgtgtt agcggaacag cgcatgctgt agccgtcagg agaaaaaata
     3481 ataaatacat tgtatacgat cctaattact cgtctggtac taaggagttc tccaatgaac
     3541 acgaattgat ttccgagtta cattacaatg tattcgggta tcataatgga ttttatatta
     3601 agggtggtct tttggggatg atggttagtg tcatccgcta tcccgacaat aagaagccaa
     3661 gagcttttcc tcaagtgact cagctttatg atcgttattt aaccaaaaac aatattaatg
     3721 acgtagctgt ttcatttcat ggtggaacat ttaatacttt agagcatgct gcaatggtta
     3781 gtcatgcaga agtaataaaa aatttgcttg agaaaggagc aaaagataat gatcatcagg
     3841 ctgctggaaa tgctgttatg tacaacaatg tagaagcatt aaaagcttta ctgggaaaaa
     3901 atagagagac agaactcttt caaaggctat ttcacgcggc tttggagagc gggagagaag
     3961 aagtttatga tgccttgttt tccatgaaag gtgccttgcc atttaattcg tctgttcaag
     4021 ccattcgagc tgcagcaaaa gggggtaatt ctgttctctt ggccaaggta atgaattatt
     4081 ttaaaagaaa tcatactgga ttttcagatc ttgaagacgc cattttttct gcaattacca
     4141 gtggaagtgt ccaatgtgtc aaattacttg ttaaccaatt aaatgaaagt aaaaaacctt
     4201 tagatgataa aaaaaggatg gagtatttgc ttgagtcaat aaagcaaaat caatcttata
     4261 tggtggctta ttttcttaaa gatatcccac cggaacattt aaaaacaatc tatatgagtg
     4321 tctctgctgt tgagagaaca aatctgcata ttttacgtca gttacagaat tatggagttc
     4381 cattttcaga tatgtcaaag gctgtttttg aacaaaaaga gcaccatttt gtaggtttaa
     4441 aacggatact aggtattttt ctgtgtaaat tcactgattt tcttaaagaa ctcttattca
     4501 atgatcgcgg aatacattgc catcgagcag aagagagtgg agtaaaaaaa tcattttttg
     4561 ccttccctaa aaaactggtt ggagttgaac aattacaaat ccccaaactt tattgaggca
     4621 cggtactaat gacaaacagt agtcatcagt ttatattaat gaaataagat ggcctaaaag
     4681 gagcttgatt cattgtgaat atcttattgg caggaggctc tggattaata ggacaagagt
     4741 tagttcgtgg aatgggagag gagcaccatt atactgtgct ggggcgtgat aaggacgcaa
     4801 tacaaaagat ttttacaaca aaagcatcct ctttgtcctg ggatgaatta aatacccagg
     4861 atgccagtcg gtttgacgct gtaattaatt tatgtggcgt caatattgcc atttcacgat
     4921 ggacaccaaa aataaaagaa gaaataatca gttcgagagt gcaaacaaca gaaaatttgc
     4981 ttcattggat tttgcagcag aatgccaaac ctcgctttta ttgcgccaat gcagttggga
     5041 tttatggttt acaggaaaat ggtgacgcaa ctgatttgac tgaagacaca gcaattaatt
     5101 gggaacaccc aaaggattac ctaagtgaag tgggaattcg ttggcagcaa gccttacaaa
     5161 aggcagagga ggcgggcctt cctgtgactt ccacacgctt tggcgttgtt ctaaaaaagg
     5221 ggaaggggtt tttagggaag ctctatccca gttttatttg cggattagga agtactattg
     5281 gggatggtcg ccaatatctt tcctggatag acagtgagga tttggtcaat gcctatcggt
     5341 ttttgttaaa tcatcctgaa attaccgggc ctgtgaatgt gacctcacca aatccatgca
     5401 tgcaaagaga atttgctaaa gcttttgcca agtctctgca taggccacta ttattgtgga
     5461 ctcctgcttt tataattcat cttttatttg gtgaaatggg tgattgttta atcaatcacg
     5521 ggcaaaaggt aaaacccaaa cgacttttgg aagctggttt tgagtttaaa tggcctgaca
     5581 ttgaaaaggc attaaagcac cagtattctg ctatatagta acagcatttt taagaggatt
     5641 tcatgatttg gagggatatt taaaataccc tgtaagttac tgaatacttg acagggtatt
     5701 taaaggggca ctaattaacg agcgctctta aagtattgtt ttaatttcat tggagaaatt
     5761 tgagagccat ctttgatagc agcggcgatt aaattaggag tgcctgtgtc ataactgcaa
     5821 atgatgtctt cgtcttgttg atgaggaact ccaggagcat tcatcatgct taacacttca
     5881 tttgatgctt ccaaagcgcc ttcctcggaa tctgcttcaa ttggagcaat gaaaaagccc
     5941 catacagaag aggtgctgta atcacttata ttataagcca gatagtaatt catgccaatt
     6001 tcttcagcca taggcaaacc ttccgccttg attacattga tatctgggca agcaccttgt
     6061 tgagaagcaa aagcagaacc ggcgagaatt aaagcgccaa aaaaagtcgc taatttagat
     6121 tgaaacatca tgttttttac tcctgagtta tttttaattc cgcgcaaaga gtatcatttg
     6181 cctagaagat ttacaataat gttgtaattt gagattggag ggtaaacgga aaacaaactg
     6241 gccttattct cggaggtatg atcattgatt actaatgcta tcgtccagaa ccagcaatca
     6301 acacagtaag gtaaatcgct aatcgttttt gaaatcaata ataactattt catggccaaa
     6361 acagttcgcg ccatcattac taaaattaaa atttgaaata ataaccatta acacgacata
     6421 ttatgcgagt tggaatcttg aggtagcaat tgtttctcaa tgacctgcaa cagcagagta
     6481 agttctcttt tatacatggt tctgtcatat ccaccttcca agccccaata gatggtgtca
     6541 atgcagtctt tgttctcgtt atacaaagtg aatatggatt catagaagta gcttagatca
     6601 ccgtcattaa agcgaaattg atgagcggca gttttcccca gcatccggcc tttaacaaat
     6661 ttaccgcaat acgctgtttc atcttcatga gaatcccatc cggtcgggag gtataaagca
     6721 attttttgcc cttttacttt cgcctctcta atcgatgctt tgaattgctc taaagcaaaa
     6781 agaagcgcag ggtgcacacc tactgatttt ctggacgtta agctgagatc caccgcataa
     6841 taacttaaat cattgtggtg ccatacatgg atgtttttcc ctacatctac tccctcagaa
     6901 ttgaattcgt tattaatgta ggaaaaatct tgttggggat atactcttga atcaaacaca
     6961 tcaatgtgac aaatcgatag atgggaaaat gagtgtctta aaatgtcaca taggccattg
     7021 tcgcgattga cgtcggttcc tataatgaca aatctaggtg gttgaacaga actcaattca
     7081 gcctgtttta tcagcatggc tgttttattg ataaggcaaa aaccgcttcc catttgggta
     7141 taggcatgat ggcttggtaa accaaaagaa aaatgggttt ttgccggatt gaacagagtt
     7201 gttgcacaat ccttgattaa aatttcaaag gtttttgggg taacgaccag atcggcattt
     7261 agttgtttaa agccaaaatg tttcgcttga atacaatatt taatgagttg ataaagataa
     7321 tcttctgaat gaacagctcg taatgctcgt aaaataatat cctcctgcgg cagcagatga
     7381 aaaagcgcat gggctttggg tgtatctcct tcatctatcg ctgtgaataa ttctagccaa
     7441 tagtctggta gcctggtttc ccgagttgta ataattggta aatttccata tttttttatc
     7501 agtgctgaca tgtgctttat acgtaagtat tgatcttctt caccgcctgc tggcatgcgg
     7561 tgcatttgct cattgtctat ttcagatggg atttgaatgg cacattcttc agcgtatgga
     7621 gtggcagaac ttttgttttt ccccgttttt gcttttttaa aaaatccttt atccttggcc
     7681 atatccattc ctaaatagct tggttaattt attatatttt agaataggcg agtgagagat
     7741 attaatccag agatttgtta agctcatgac tatcaagaca agaggcgcta ctttatttat
     7801 aatgcaaata gagtttatgg acagaatttc attttgttct gactcgacaa ttcctccgtt
     7861 atccaattaa gtcgagggtc tatggtacaa catgatataa tcaaactgcc tccaaaacct
     7921 tatggtgctc atctaatcac taatgaagtg atgcaaaaaa ttacgttgag gggtaccgga
     7981 ttattacatt tatttattca gcatacctct gcggcacttg ccttaaatga aaatgcttcc
     8041 cctgatgtat tgattgatct aaaccatttc tttactcaac ttgttcctga acatcagcat
     8101 tatcgtcatg atatagaggg gccagatgat atgcccgccc atattaaatc aattttgtgt
     8161 ggctcatctt taactattcc atttcaggaa aacaagttgc tattgggtgt ttggcaaggc
     8221 atttatttaa tggaattcag aaaacaagca ggagcaagaa caattgttgc cagtcaattg
     8281 atttaggatc atagggcata actctactca ccattgaatt aatctgagca atgcatgtat
     8341 ctttctgtgt ttcattcaca ggataaatac ctcctgttct taactatact ttatattatt
     8401 ttgaaaccag gacagcctca tgacgaatga tcattacgta tctaaaaaaa agatgtttgc
     8461 tgtggttttt tgtgtttttg cgcctaatga ggttttatta gctaatgata ctgctgtagg
     8521 tggggaggga tctttaccca ttcctgtttc tcagccgcat ataaaaatgg tcagtgagtt
     8581 aatcaggatc agcggaaaga atttaaatga gcctcagatg aatggttcct ggcattataa
     8641 ttgtaatttc acttttaaaa atacattaaa taaagaagtc accatttcaa tggcttttcc
     8701 ctttcctata aacgatggga attcagaaat tgccctgcca gcaggacagc agactaatgt
     8761 tggtcaagca ttggtttatg attttctggt tacagtcaat gataagcaag tttcagcaca
     8821 gcgcggtaat atagccccgg atcagaataa aggtctttac tatgaggatg cctatttttg
     8881 gaaaaccacc tttccaccct tggcaacagt caatattcat catgattatt cgacgggagc
     8941 cacttatgat gtgatggggt atcattgggt tcgttatgtt ttaaaaacag gtgctctttg
     9001 gcaggatagc agtatcggac atactcgact tgaagtcatt cccaatacac caacgcgttt
     9061 atgctctgag atagatcaaa aggcagacta cctcaatcct accccatcag ggatgagcat
     9121 ttcaggctca cgagcggata gaaaatacat ctgggattta cggcattttc aaccccaagc
     9181 agatttgtca ctttgcttat ttacggggat tagttatgtt cgctataagg tgatttatcc
     9241 ttggttaaat tcagatgatg cattaagcaa attggccaga ttgagtaata aagaattacg
     9301 attcttacga aatactattt atgcgcaata tgggcgtcaa tttcagtctc ctgatttaca
     9361 agaatatttt agcaagaaat ggtggtatgt accaaatcct gactattctg accgtatgct
     9421 caatgaagaa gataagaaat tattgtcaat gattaatcag gcaaaataag ctcaatcgct
     9481 gataggctct ttataataaa tcccatatga ggcctctaaa ttaataaaag ctgattttga
     9541 caggtgacta ttaccacgcc ggtctatttt tgttttatgc caattcttgt gtttgttgca
     9601 ggatcgcgtg agaaagcatt tttgcaattt tattgagatg cattcttact ataatgtcct
     9661 actaattcag ccgtatctat gatatctcct ttcatagcat tcaccacatc ctgagcagtg
     9721 gcttctgggc caagactaag gtaagtgtct aaagcataga gtgtaaaata gtaacggtgt
     9781 gtacctgaag gcgggcaggg accactgtaa ccagtggtat tccagctatt taacccactg
     9841 gttgcgccag caggagttgt tgtcccttct gtgagctgtt tcacttgagc agggatatta
     9901 aacaataccc aatgtatcca attacctgta ggagcatcag gatcatgcac tattaacaca
     9961 taagattggg ttcccagatt gggatcatgc catattagtg ggggagaata atttaaattg
    10021 ctgcatgtga atttttgagg aattgctgca ttcgatgcaa atgccggact ttccaatgtt
    10081 aatcctgcag aaaaacacgg tgtaacaagt aatgcagagc tgactaatgt caagacaaga
    10141 tttttaaata tggccatcac tttccctgct tctattgttt ccttacctga aattattgag
    10201 agtctagtct tttatagact cccttaaatt atagattacg attgatacac ttgtgatttg
    10261 tttttcatca ggatttttac caaaagcacc aatttttttg tttaaaaacg tttaattcgg
    10321 tcatttattt tatctaaact ccctcattaa aagcattttt cctcaacatt ggtttttttt
    10381 gtaagtccca ttcatatgag aaatgccttg tgctgttgtt tataaaattt tctaaaccta
    10441 taatgaaact ccagtatttt taaaggaata atttcaatga aagcaatagg ttactaccac
    10501 tctttaccca ttacagataa taaagcattg atggacctgg atgtttctgt gcccaaagca
    10561 actggccacg atattttagt tgaaatcaaa gctgttgcag tgaatcccgt agataccaag
    10621 gtgcgtcgta atgttcaacc agaacctcat cagcccaaaa tattaggctg ggatgcttcc
    10681 ggggtagtta ctgaaattgg atcggaggtg actatgttta aaccaggtga tgaagtgtgg
    10741 tatgccggtg atttaaccag accagggtct aattgtgaat ttcaccttgt tgacgaacgc
    10801 atcgcagcca aaaaaccaaa atcgttaagt tttcaagagg ctgctgcatt acctttaacc
    10861 agtttaacag cttgggagct tttatttgat agattgcaat tatcagcaga agataaaggt
    10921 attctattaa ttactggagc ggctgggggg gttggttcta ttttggtaca actggcaagg
    10981 caacttaccc agttaactat catcggaaca ggagcccgcc ctgaatcaat tcagtggatc
    11041 ctggataatg gggctcacca tgtcattgat cacactaaag acatgaaggc tcaattgcag
    11101 aatttacatt attctgaagt ggattatgtg atcagtttga cccataccga tcaacatgcc
    11161 aaatcgctag ttgaggtcct gaaacctcaa ggaaaatttg ctttgataga tgaccctcag
    11221 aatttggata ttaaattatt taaactaaaa agcatttcta ttcactggga aatgatgtac
    11281 acccgctcta tgtttaaaac agcagatatg ataaagcaac atgatatttt aacagaggtt
    11341 gccagattgg tggatgccgg taaaatcaag acaactattg ccgaatgtct gggattgatc
    11401 aatgctgaca atttgagaag agcacatgct ttattggaaa gtggcacttc cagaggcaag
    11461 atcgttttga atggattcaa gtcaatttag ttattctcga aacagcgacg atgcctggaa
    11521 tgaaacagat agagtcactt gatttttaaa tgattctcta aatagcctac gtgcctcagc
    11581 ttgtctgagg catccggaaa gtgcttggtt ggtatcgctg acaagcagca gtacccggag
    11641 gatcactcat ttagtttatc taaactcctc attaaaaaca ttttttgcct tgaccttgga
    11701 gttttacgca aatctatttg tgttaaaaaa atatctttta attaaaaatt ggacatgatg
    11761 ttttgtcgtg ttctatatta attaatacag ggaataaaaa atataagatc aattcaatca
    11821 caaggaaaaa ctatgattat ttatctgcta tggttattag cagtaacatc atttaatttt
    11881 tcaacttcta cgattccagt gtcaaaagcc gagcctcaag ggaatctttt atacagtgaa
    11941 ataccgtcaa tcaagatgcc tttgaatgaa ataaaaacat tgttacaaaa agagggtaat
    12001 tctcttcaac ctgccgttat tgataaagtg ataacgacca tacaatgcgc taatgcttat
    12061 caagtggata gaaataatat attgactatt attgattatt ccatgccttc taatcaaaaa
    12121 cggttatggg tgtttgattt ggataagaaa gaattattat ttcatactta tgtatctcat
    12181 gggataaagt caggcactct tttaacagat aaattttcaa ataaatttga tagtaaggcc
    12241 agcagtattg gtgtttataa aacagaacaa gtctattatg gtcgagaagg attgtcttta
    12301 cgattagtgg ggttggatac caaatttaat gataatgcct ttaatcggta tattgttatg
    12361 catggcggat ggtatatgga tgaacaattc atcaaaagat atggtcggcc tggccgtagt
    12421 tggggttgtc ctgcgttacc tttgccaatt aaaaaacaaa ttattgatac tataaaagat
    12481 aattc
//
LOCUS       DACTJE010000091        12475 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.091, whole genome shotgun sequence.
ACCESSION   DACTJE010000091 DACTJE010000000
VERSION     DACTJE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12475
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.091"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(423..650)
                     /locus_tag="I9760_001905"
     CDS             complement(423..650)
                     /locus_tag="I9760_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAT5043669.1"
                     /translation="MKITDEELKKIISKLSGSKADEIQDDTALIEDLHLDSLKIVELL
                     AILSEEYQLEVSEEDAMNFHTYKDLYDFTQA"
     gene            complement(660..1883)
                     /locus_tag="I9760_001906"
     CDS             complement(660..1883)
                     /locus_tag="I9760_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HAT5043670.1"
                     /translation="MHQEHPGQTDTPKIDPWLIFVILMLMALMQTTTDQYIPSLPAIT
                     TALNSTEASIQLTISMFMLGLSLSHIFYGPLSDKIGRKPPLMFGVGLSILGSLVCFVA
                     PNVSVLILGRFLQGFGIGCCNSVGRSLVRDLFTDRLLAKIGSYVGVVSIFIMAASPTL
                     GGFIQEMAGWKANFMFLFVFGILVWLLAFCILPETNKHLNPEATKIKVMAKNYFTLLR
                     SKIFVGYTMCACFAFAGMVSYLTIAPFLFQNTMGLSPLEFGRLTFFIAGAICFSGIVN
                     SQLVMRKGISFMVCTGVLFMIVGGLSLLLITILGWSQIYFIMISMTLFSAGAGFTFIN
                     AFAGAFHPFPQMAGTVGALYASMQDLSAALTSGFIAGIKGYGQYSLAIILLILGLSSL
                     VAWYYLGLQDDAISN"
     gene            2037..3239
                     /locus_tag="I9760_001907"
     CDS             2037..3239
                     /locus_tag="I9760_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HAT5043671.1"
                     /translation="MDINLMVFASPLFLILIGIETWVAYYKKSGVYQLNDSVNNFSSG
                     ILEEIGALPIKGLIIYSYYYLYEHYAIFNVNTQSIFSWLLLWIGVDFFYYWYHRVSHR
                     NSFFWIGHSVHHQSEHFNLSVALRQGYFQTLTSWVFYLPLALIGFPTWMFVIVASVNT
                     IYQFWIHTESIRKMGWFEKIFNTPSHHRVHHGKNPQYIDKNYAGSLIIWDKLFGTFEP
                     EEAPADYGVTEPLDSWNPFYANIKVVKDILYYGKGLKSKWDVLRAFFMPPEWIIHRLQ
                     QEKSPLSKKTTIKKNTTSPGIYMLTNTAFAVLLYTYLSLVFDPAMVTSWLIGALILLT
                     LYTLGRIADGHSNTLYLEISRATLVLLILLLFKNTLLLSLGCSVLFYVINYALCTTFP
                     YKRTASSF"
     gene            3392..5734
                     /locus_tag="I9760_001908"
     CDS             3392..5734
                     /locus_tag="I9760_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HAT5043672.1"
                     /translation="MHTIANETPVQKDHSFDPITNVLKWVLLVTAILCFLILIWGTVK
                     TYQLVPPLPDRFVTSTGETVMTSQDIVEGKAGFQRADLMDYGSLYGMGSYFGEDYTAK
                     YLVRLGELVQEELAKIRFNKAYSELTESEQFVINKEMQNSLQRLNLNKKTSILTPAIA
                     TSIRQLQAEISQSLLNHNFMAGYTQAYSLNPDTALQTANFLIYSSLTTIARRPNQNIS
                     YTNNWPYEPSVGNTPSSGTFYWTWISFCFVFMGFGAVLYIYHRYLSDVDHGPMEPIFS
                     GFKPLTQSQRKVGQYFLVAALFLLLQIAVGAIMAHYYAERESFYSINVNTWLPFNFLR
                     DVHIQTPIIWIGLSWISAGIFMAPIISGKEAKGQGFLVDFLFYVTLLVVGGAILGNYL
                     GIMGYIDKTWFWLGNQGLSYIQLGRLWQIGFCIGLFLWSFIVFRGMWPTWEGLKNATI
                     EFWTGRIKLENLFWASTINVAVLYCFGMIPLTGIEKSFTITDFWRWWVVHLWVEQSFE
                     FFAACATAYVLMGTGLVSRKAAERTVFFESILIFLGGVIGTGHHWYWTGTPEIWIPLG
                     SMFSFIEVLPLVLLIIDAIQHRQLIKRQGMFTYNLAYLYILGAAFWNFVGAGVFGGGT
                     LNAPLINYYEHGTFLTLNHAHTALFGAFGLLALGLIYFCLRYAAGESKAWSDRLGVYA
                     FWLYNAGLFLWIVLNFFPIGWSQLMAVYEHGLSYARSLEFYNTTLLWQWLRFPGDVVF
                     ALGALLMAYDFIVKIGPFFPKFARNRRFIAGPPKATDPSL"
     gene            complement(5923..7755)
                     /locus_tag="I9760_001909"
     CDS             complement(5923..7755)
                     /locus_tag="I9760_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5043673.1"
                     /translation="MSDLQQLPKTILRFSWHFIKKSPWLYALFFIAPVVMVLETNVIP
                     YALKMIIDGITNHKGGKDSIFQDIAPALYLGGVAWFGVILVLRLHNWWQAYLVPQFQA
                     NIRMTLFGYLIQHSHHFFSNQLAGNLANKISDIPRSLEAIRKIVSCNVNTTFAAIVVS
                     IVLMLTINPWFAFILLSWIIIQLFISFYSAKEINKLARVNAEDKSILKGKIVDSLNNI
                     NTVKLFVQNEYEKKYVWKTQNREVQSNKRLVLFINFFRLLVDVPVSIMLIAMIYSVIS
                     FWQRNLISTGDVVFIFATSFAIMNQMWSLSNSLCDLFVEIGIVKQALAILSQPIEVQD
                     IANAKELKVTHGEIRFENVTFRHRDGQVLFTNKSLVIPAKQKVGLVGYSGGGKTTFIN
                     LILRYFDVDSGRILIDNQDISEITQYSLRKSISMIPQDTNLFHRTLLENIQYGKEDAD
                     RDEVVTAASLANCMDFIQNLPQGFNTIVGERGTKLSGGQRQRIAIARAFLHHTPILIL
                     DEATSQLDSLTEECIQESLQPLCEGKTTIVVAHRLSTLLNMDRILVFDKGAIVEDGTH
                     QELIENNGLYKSMWDAQTSGFLPVEQEDETELAKEFDRPYLAQV"
     gene            complement(7920..8306)
                     /locus_tag="I9760_001910"
     CDS             complement(7920..8306)
                     /locus_tag="I9760_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HAT5043674.1"
                     /translation="MYTIIVCLFIAILLPYLAKVPVAYAMHKAGGYNNRYPREQQARL
                     QGFGARALAAHQNAFESLLIFATASLTALATNSVTLMTQYFAIAYIISRLFYHLFYLL
                     NWSSLRSTVWFLGVICCLSILWLSIP"
     gene            8517..12374
                     /locus_tag="I9760_001911"
     CDS             8517..12374
                     /locus_tag="I9760_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2239 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043675.1"
                     /translation="MKMNEYIELYERHKYEGNNTIDSLILTLNSLKQSRESWGQENKL
                     AKYLGFGLRIDKHPFFQPKVTQKNLHVIETVIEKLNALKESPDYQQEGETSTTRLDIL
                     GILGERFNELSKNGKTRGLFNYVMFNEGSIDFSMRESTLVEPSPLEILTGNEEPYEDE
                     QTWEPPYIHKLLDSKGIPYTIFDSLETNDLNLENDLGDHVKWAYDNIYRYSYPDSHQE
                     STIARFHHGIQHVTRAANYACAFANLYRRHGNEEAQNLTETDIKLIQIALLFHDSARE
                     GDDEDLWDHESAIFLYYYLTRVLHIDAETARFVAEATANKDPSPENGYFQLIEDEYGQ
                     LFWQFAKYEEGDFPAKNIYQKIIHDCDCLDIIRARNQYDATYLDFYKDIASKNNLALE
                     EMAELVIEARGLIYSQGDSHITKKAEIKAGYENEEAHARIIRDMQSEAYPILSTLYGK
                     LLSLEELQAMTLVNLTPFDETAGMAGENLAAALREGRVLARGITTPSAMPTPRPNKPT
                     LTDETLAEREMRKSMRSDNINIKSRKEAPNLKYKNPLRSTSILGYGSGVYPAAGMLIF
                     NPDIGQIKRISAEDFDSGRGQKNHLAHLQEKEYKESREILERELHDLHHKMKLGLFGK
                     KTQLGSNYVELLYDINKYDAIFFTNDPTIGNEIAYNDFNPAHPLSPLLQAIYLQKQYA
                     RQYEETRKAYYERYGQERGETLFLERFGPNQFLPIYEYSGLRNKLTPYDTEKLTDENI
                     VNMWVEMCSDFMRNALSHMGRDNSYDFYDELLYNMSIEDIKVLSMYKTKGNILAKVNQ
                     PADLSYNVELREKITDTIAKAKEKIIAEYEEAVFKRLQDNQLSPFSKEAFLALEYSPR
                     LRDLCSDKIKDDLLRFIYSPDGVISYKLSDIASTSLREKTTTLDIQHCSTDQRNFLCK
                     NQFLQAFILCQQLGLEGEIQFLRNKVNELAIQVINDQSAFITGSKYELQSGLHQMINL
                     NNIMTLIDKDNHLRGNIESILLEIVSKLIDTYTNERLNLYESYMLLKTLENAGIPDVK
                     LKHYFRPVLNKANETLHERKPHDWGHYCSIARLVGDEAKPYIYSWLNDYDKPFYSEIN
                     SMLETFKNAGIVFDDENLDVFKSLLEKIHYNDVAYNYVRLSYWLRVMNNLQSLASESH
                     YSGSQLEILKNQFDRVIEKLIKTTKSEYEHASYYSQEQKCYRQLTSFAEEINTILASD
                     FCLPVPDSLITEFNLTLIAFSEMDLSQTKVDESKFHLLKQLHNKLPNPEKREQAMEKL
                     TESMDTKKQVEKDQNKNISAAGL"
BASE COUNT         4031 a         2397 c         2327 g         3720 t
ORIGIN      
        1 taaaatctgg agaaaaggta cttttggtgg cagcaggtgg aggaggaatt gcagcctgtt
       61 ctttattgga agttgtctga tttggcttca ggaattgttt gggtcgagac ttgcgaaaaa
      121 ccccatggtc aagggaaacg attagcaaat tctaatttaa caaattcaat tttatataaa
      181 aataaataag ataatctttg aaattttttc gataatccct tgtccttgtt cgtttgggct
      241 gaggaggcgt ttacgccgtc tcgaagcctt tacgaaaaaa ataacaaggc ttcgagacag
      301 cgcttaagcg cttcctcagc ccgaacggat agttgatcta tacgaataag caattttaga
      361 ttggcggccc tggatcaaaa tgccccaact cttactacaa aaaatggaat aactggcaac
      421 aatcaagctt gcgtaaaatc atacaaatct ttataagtat gaaaattcat cgcgtcttct
      481 tcactgacct ctaattgata ttcttcactg aggatagcca aaagctcaac aatttttaaa
      541 gaatccaaat gcaaatcttc tattagggcc gtatcatctt gaatttcatc ggctttagag
      601 ccagataatt tgctgataat ttttttcaat tcttcatcgg taattttcat taatacccct
      661 taatttgaaa tagcgtcatc ttgcaatcca agataatacc atgccactaa agaacttaaa
      721 cctaaaatca gtaatattat agctaaagaa tattgaccat accccttaat tcccgcaata
      781 aaaccactgg tcaatgctgc acttaaatcc tgcatgctgg catataaagc cccaaccgtt
      841 ccagccattt gcggaaaagg atgaaacgca ccggcaaagg cattaataaa tgtaaatccg
      901 gcgccagcac taaataatgt cattgaaatc ataataaaat agatttgtga ccaacccagt
      961 atcgtaatta ataacaggct taaaccaccg acaatcataa aaagaacacc ggtacaaacc
     1021 ataaaggata ttcctttacg catgaccaac tgactgttga caatcccgct aaaacagatg
     1081 gcgcccgcaa taaaaaaagt taaacgtcca aattccaagg gactcaatcc catggtattt
     1141 tgaaataaaa atggcgcaat cgtaagataa gaaaccattc ccgcaaaagc aaagcaagca
     1201 cacattgtat atcccacaaa gatcttgcta cgtaataagg tgaaataatt cttcgccatc
     1261 actttgattt ttgtagcttc aggatttaag tgtttattcg tttcaggaag aatacaaaat
     1321 gctaaaagcc aaacaaggat tccaaaaaca aataagaaca taaaattggc tttccaccca
     1381 gccatctcct gaataaatcc tcctaaagtg ggtgaggctg ccataataaa aatactgaca
     1441 acaccgacat aagaaccaat tttagccagc aaacgatcgg tgaataaatc tctcactaaa
     1501 gatcgcccca ctgaattgca acacccaatt ccaaacccct gaagaaatcg acccaagatt
     1561 aacacactga cattgggcgc aacaaaacaa accaaactac ctaatatact tagcccaaca
     1621 ccgaacatta acggaggttt gcgtcctatt ttatctgaca aggggccata aaagatatga
     1681 gataggctta aacccaacat aaacatagaa atggttaact gaatagacgc ctcagtgctg
     1741 ttcaatgctg tggtaatcgc tggaagagaa ggaatgtatt gatcggttgt tgtttgcatc
     1801 aaagccatca acattaatat gacaaatatt aaccaaggat ctatttttgg ggtatcggtt
     1861 tggcctggat gttcctgatg catcaaatat cctttagaat gatttctaac aaggctgata
     1921 acaatatcat tatttacaag caaaacaaaa gtattagaaa ataaggcatt ggcctcaacg
     1981 tctgcaaaat agaaaatacc tattgcaaag cgtctatcac tgttcaaagg atatttatgg
     2041 atataaattt aatggttttt gcctcacctc tttttttgat cctcatagga attgaaactt
     2101 gggtagccta ttataaaaaa tccggtgtct atcaactgaa tgattcggtc aacaatttca
     2161 gcagcggtat ccttgaagaa attggcgcac tccccatcaa gggcctgatt atttatagtt
     2221 actattattt atatgagcat tacgctattt ttaatgtgaa tactcaatcg attttttcct
     2281 ggttactttt atggatcggg gttgattttt tctattactg gtaccacaga gtaagccaca
     2341 gaaacagttt cttctggata ggtcattcgg tccatcacca aagtgagcac tttaacttat
     2401 ctgttgcctt aagacagggc tatttccaga cattaacttc ctgggtgttt tatttgccct
     2461 tggctctgat tggttttcca acctggatgt ttgtcattgt cgcctctgtc aataccatct
     2521 atcaattctg gattcatact gaatccattc ggaaaatggg ttggtttgaa aaaatattta
     2581 atactccgtc tcatcacaga gttcatcatg gcaaaaaccc tcaatacata gataaaaatt
     2641 atgcgggaag cctgattatc tgggataaac tgttcggcac ttttgaacca gaagaagcgc
     2701 cggctgatta tggagtcacc gaacccctgg attcatggaa ccctttttac gccaacatca
     2761 aagtggttaa agatatcctg tattatggta aagggctaaa aagtaaatgg gatgtgttac
     2821 gcgcgttctt tatgccgcca gaatggatca ttcatcgctt gcaacaagaa aaatcccctc
     2881 tctcaaaaaa aacaacgatt aaaaaaaata cgacctcacc tgggatttat atgttaacca
     2941 atactgcatt tgcagtgtta ttatatactt atctctctct tgtttttgat cccgccatgg
     3001 tcacatcctg gctgattggc gcattaattt tattgacact ttataccctt ggccgaattg
     3061 cagatggcca tagcaatact ctttacctgg aaatatcgcg cgcaacattg gtacttttaa
     3121 tattattgct atttaaaaat actctgttat tgtcgctcgg ttgcagtgtt ttgttttatg
     3181 tcatcaatta tgccttatgc actacttttc cttataaaag aaccgcatct tctttctgag
     3241 aagcagacac tattcaacaa ggattaaccc tgtgggtact ggagatgtat ggttttctaa
     3301 gagtcagttt tgtagctaac atgaattgtt tcttgtaaag atcctcttta taatcaatgc
     3361 cgaattgaac cattccgcta aggatagcct gatgcatact attgcaaatg aaacacctgt
     3421 tcaaaaggat cactcatttg acccaataac taatgtttta aaatgggttt tactagtcac
     3481 tgccattctt tgttttctaa tcctcatctg gggcactgtt aaaacctatc aattggtacc
     3541 tcctttacca gaccgttttg tgacctctac aggcgaaacg gtgatgacat cacaagacat
     3601 cgtggaagga aaagcaggtt ttcaacgcgc tgacttaatg gactatggca gtttatacgg
     3661 tatgggatcc tattttggtg aggactacac ggcaaaatat ctggtgcgtt tgggagaatt
     3721 ggttcaggaa gaattggcaa aaattcgttt taataaagcc tattctgaat taacagaaag
     3781 tgaacaattc gttattaata aagaaatgca gaactcactg cagcgcctta atctcaataa
     3841 aaaaacgagc atcctgacac cggcaattgc cacctcaata cggcaattac aggctgaaat
     3901 cagtcaatcg ttattgaatc ataattttat ggcgggttat acccaggcct atagcttaaa
     3961 tcctgatacc gcgcttcaaa ccgcaaactt tcttatttat tcctctttaa caacgatagc
     4021 tcgccgccca aatcaaaaca tatcttacac caataattgg ccatacgagc caagcgttgg
     4081 caatacccct tcatcaggga cgttttattg gacctggatt tcattttgct ttgtgtttat
     4141 gggatttggt gcggttctct acatttatca tcgctattta agtgatgttg atcatggacc
     4201 catggaacct attttttcag gtttcaaacc cctaactcaa agccagcgta aagtaggtca
     4261 atatttcctt gtagccgcgt tgtttctttt gctgcaaatc gcggtcggag ccatcatggc
     4321 tcattattat gctgagcgcg aaagcttcta tagtattaat gtcaatactt ggctgccttt
     4381 taatttcctt cgagacgtcc atatccagac tcccattatc tggattgggc tttcatggat
     4441 aagtgcaggc atatttatgg cacccatcat tagcggtaag gaagccaaag gacagggatt
     4501 tttagttgac ttcctgtttt atgtcacctt gctcgtagtg ggtggagcca tccttggcaa
     4561 ttacctgggg attatggggt atattgataa aacctggttt tggttgggca atcaaggttt
     4621 gtcttacata caattaggaa gactgtggca aatcgggttt tgcattggat tgtttctttg
     4681 gagcttcatt gttttccgcg gcatgtggcc tacgtgggaa ggattaaaaa atgccaccat
     4741 tgagttctgg acaggtcgaa tcaaacttga aaacctgttc tgggccagca ctatcaatgt
     4801 cgctgtttta tattgctttg ggatgatccc cttaacagga attgaaaaat cattcaccat
     4861 aacggatttc tggcgttggt gggttgtgca tctatgggtt gaacaatcct ttgagttttt
     4921 tgctgcttgt gccaccgcct acgtacttat gggaactggt ttggtttcca gaaaagccgc
     4981 agagagaaca gtattctttg aatccatttt aatcttccta ggtggggtaa taggtactgg
     5041 tcatcattgg tactggacag gtactcccga aatttggatt ccgctaggtt ccatgttttc
     5101 ttttatcgaa gtcttgccac tggttcttct tattattgat gcgattcagc atcgacaatt
     5161 aattaaaagg caggggatgt ttacctacaa tcttgcttat ctttacatat tgggagctgc
     5221 gttctggaat tttgttggtg ccggtgtgtt cggtggaggt acacttaatg cgccactgat
     5281 taattactac gagcatggaa cattcttgac tttaaatcat gcccacactg ctttatttgg
     5341 cgcatttggg cttcttgctc tcgggttaat ctatttttgc ctgagatatg ctgctggtga
     5401 aagcaaggca tggagtgata gattgggtgt ttatgccttt tggttatata atgcaggtct
     5461 ctttttatgg attgtcctga attttttccc gatcggctgg tcacaattaa tggcagtata
     5521 tgaacatgga ctatcctatg ccagaagtct tgagttctat aacaccacat tattatggca
     5581 atggcttcgt tttccgggag atgtcgtatt tgccttggga gcccttctta tggcttatga
     5641 ttttattgtt aagattggac cttttttccc gaaatttgcc cgtaatcgtc gctttatagc
     5701 tggccctcca aaagcaacag atccaagctt atgaccaatg atagcaaaga caaagccagt
     5761 gtattttttt gaaaaattat tttgagaaac cgtgtttaga agtgaatgtc gacacccaga
     5821 tgatgggtgt cggcttgcaa tcaaattcaa ggtaagagat cagcctctac ctggaacggg
     5881 cgtatttggt tcatcttgaa cgacaattgt tttttggtta aattacactt gcgccaaata
     5941 aggccggtca aattctttag ctagctcagt ctcatcttct tgttccacag gcaagaaacc
     6001 tgaggtttgc gcatcccaca tggatttata taatccattg ttttctatca actcttgatg
     6061 tgtgccatct tctactattg ctcctttatc aaaaaccagg attctatcca tgttaaggag
     6121 ggttgaaagc cgatgtgcta caacgatagt tgtttttccc tcgcataatg gttgcaagga
     6181 ttcttgaatg cattcttcag ttaaagaatc cagttgtgaa gtggcttcat ctaaaatcaa
     6241 tataggagta tgatgcaaaa acgctcttgc tatagcgatt ctttgtcttt gcccccctga
     6301 caacttagta cccctttccc ctactatagt attaaaaccc tgaggcaaat tctgaataaa
     6361 gtccatgcaa tttgctaaag aagctgctgt tactacttca tccctgtcag catcctcttt
     6421 accatattga atgttttcta ataaggtacg atggaataga ttggtatcct gaggaatcat
     6481 actgattgat tttcttaaag aatattgcgt gatctcgcta atgtcttgat tatcaatcaa
     6541 tatccttccg ctatccacat caaaataacg gagaattaga ttgataaatg ttgttttgcc
     6601 tcccccggaa tagcctacaa gcccaacttt ttgtttagcc gggatgacca acgatttatt
     6661 ggtaaataat acctgaccat ctctatgacg aaaggtcaca ttttcaaacc tgatttcacc
     6721 atgcgtgact ttcaactcct ttgcattggc tatatcttga acttcgatag gttgggataa
     6781 tatggcaagt gcttgcttga ctattcctat ttcgacaaac aaatcacaaa gcgaattact
     6841 gagactccac atttgattca taatggcaaa tgaagtagca aatataaaaa ccacgtcacc
     6901 agtgctgatt aaatttcttt gccagaagct gatgactgaa taaatcatgg cgattaacat
     6961 aatactgaca ggaacatcga ctaaaagacg aaaaaaattg ataaaaagaa ccaggcgctt
     7021 attactttga acctctctat tttgtgtttt ccaaacgtat tttttttcat actcattttg
     7081 gacaaataat ttcacggtat tgatattgtt caaagaatca acgatctttc cctttaaaat
     7141 acttttatct tccgcattca ccctagcaag cttattgatc tctttagctg aataaaagct
     7201 gataaataat tgaattatga tccagcttaa caaaatgaat gcaaaccagg gattaatggt
     7261 taacattaaa acgattgaaa ctacaattgc cgcaaaagta gtgtttacat tacaagatac
     7321 gattttacga atggcttcca atgatcgagg tatatcactt atcttattag ccaggtttcc
     7381 cgctaattga ttagaaaaaa aatgatggga atgctgtatt aaataaccga atagggtcat
     7441 acggatattg gcttgaaatt gaggaacaag gtaggcttgc caccaattat gcaagcgcaa
     7501 caccagaatc acaccaaacc aggcaactcc acccaaatac aaagccggtg cgatgtcttg
     7561 aaatatgctg tcttttcccc ctttatggtt tgttatccca tcaataatca tttttaaagc
     7621 atagggaatc acattcgttt caagcaccat caccactggt gctatgaaaa acaaagcata
     7681 aagccaggga ctttttttta taaaatgcca ggaaaatctc aggatagtct taggcaattg
     7741 ttgtaagtca gacatattca catccttgta aaaaatctgt aacttcttat ttaagataac
     7801 aaagtcaaag acaaagagta agtgtctata accaattaga tgctaaattg atggattttt
     7861 gcgccttaaa tcatcactcg gtgaattttt ttgaataaag acataccttg ccaaaatttt
     7921 taaggaatac taagccataa aatagagaga cagcatatta cacccaaaaa ccatacagtg
     7981 gagcgcaggg aagaccaatt cagcaaataa aacaaatgat aaaaaagccg ggaaataata
     8041 taggcgatag cgaaatattg ggtcattaaa gtgactgaat tagtggccaa tgcagttaat
     8101 gaagctgttg caaatatcag taaagactca aaagcatttt gatgcgctgc cagagctctt
     8161 gcgccaaacc cttgcaagcg agcttgttgc tctctgggat aacgattatt atagccaccc
     8221 gccttatgca tggcataagc tacaggaact tttgccagat aaggtagcaa tatagcgata
     8281 aacaagcaaa caattatagt atacatggtt atagagtatc tcaggcatat attaaaaata
     8341 actaaatgac atattaacca agatgttctc tcaactcaat cgggcaaatt taattgtagt
     8401 ccactttagt tagcaggaat actccttaaa tagcttttaa attacttcac ggccagtttt
     8461 tggtcaataa ttaataaaat tgctataatt ttattctaaa gtatcttttt ggtacgatga
     8521 aaatgaacga atatattgag ctttatgaac gccataaata tgagggaaat aatacaatag
     8581 attccttgat tctgactttg aatagtttaa aacagagcag ggaaagctgg ggacaggaga
     8641 ataagcttgc caagtattta ggctttgggt tgcgtattga caaacatccg ttttttcaac
     8701 ccaaagtgac gcaaaaaaat ttacatgtaa tagaaactgt tatcgaaaaa ttaaatgctt
     8761 taaaagagag tccagattac cagcaagaag gagagacatc aacaactcgc ctggatattt
     8821 tagggatatt aggagagcga tttaacgaac tatccaagaa tggtaaaacc cgtggattat
     8881 ttaattacgt catgtttaat gagggttcaa ttgatttctc aatgagagag agcactcttg
     8941 tggagccatc acctttagaa attcttactg gaaatgagga gccttacgaa gatgaacaaa
     9001 cgtgggaacc tccctatatt cataaactac tcgattcaaa aggaatacca tatacaattt
     9061 ttgattcttt agaaacgaat gatttaaatc ttgaaaatga tttgggcgat catgtcaagt
     9121 gggcttatga caatatttat cgttattcct atcctgactc gcaccaggaa tcaactatag
     9181 ctagatttca tcacggaata caacatgtta cccgagctgc aaactatgca tgtgcatttg
     9241 ccaatttata caggagacat ggtaacgagg aagcccaaaa tttaacagaa acagacatca
     9301 aactcattca aattgctttg ttattccatg attcggcccg agaaggagat gatgaagatt
     9361 tatgggatca tgaaagtgct atctttttat actattatct gacacgtgtt ttacatattg
     9421 atgcagaaac agcaagattt gtagctgagg caactgcgaa caaagatccg agtccggaaa
     9481 atggctattt tcagctcatt gaggacgaat atggtcaatt attctggcag tttgcgaagt
     9541 atgaagaagg agattttcct gcaaaaaata tctatcaaaa aataattcat gattgcgatt
     9601 gtcttgatat catcagggca agaaatcaat acgatgccac ctatcttgat ttttataagg
     9661 atatagccag caaaaacaat ctcgcattag aagaaatggc cgaacttgtc atagaagcca
     9721 gaggcctcat ctatagccag ggtgattccc acattactaa aaaagcagaa atcaaagcag
     9781 gttatgaaaa cgaagaagct catgcccgca taatcaggga tatgcagtca gaagcttatc
     9841 caatactcag cacattgtat ggaaaactgt tatctttaga agaattacag gctatgacct
     9901 tggttaatct tactcccttc gatgaaacag caggaatggc tggagaaaac cttgctgcgg
     9961 cattgagaga agggagagtt ctggcccgtg gcattactac accaagtgcc atgcctacac
    10021 ccagacccaa taaaccaact ctaacagatg aaacattggc cgaaagagaa atgcgaaaat
    10081 ccatgcgctc tgacaatatt aacatcaaaa gcagaaaaga agcacctaat ctcaaataca
    10141 aaaacccgct gcgctcaacc tccatactcg gttatggaag tggagtctat ccagctgcag
    10201 gcatgttgat cttcaatccg gatataggtc aaattaagag gatttcagct gaggattttg
    10261 atagtggccg aggtcaaaaa aaccatctgg cccatttaca agaaaaagaa tacaaagagt
    10321 ctcgtgaaat tctggaaagg gaacttcatg atcttcatca taaaatgaaa ttgggcttat
    10381 ttggcaaaaa aacgcaactt ggctcaaatt acgttgaact tttatatgac attaacaaat
    10441 acgatgccat tttctttact aatgacccaa ccataggaaa tgaaattgcc tataatgatt
    10501 tcaatccagc tcatcccctg tcccctctct tgcaagccat ttatttacag aagcagtatg
    10561 caagacaata tgaagaaact cgtaaagctt attatgaacg ctatggacaa gagaggggtg
    10621 aaacactatt cctcgaacgc tttggcccta accaattttt accaatttac gaatattccg
    10681 gcttaaggaa taagttaacg ccttatgata cagaaaaatt aacagatgag aatattgtta
    10741 atatgtgggt ggagatgtgt tctgatttca tgagaaatgc tctgtcccat atgggtagag
    10801 ataatagcta tgatttttac gatgaacttc tttataacat gagtattgaa gacataaaag
    10861 ttttaagcat gtacaaaaca aaaggaaata tattggcaaa agtgaatcaa ccagctgatt
    10921 taagctataa tgttgaatta agggagaaaa ttactgatac catagccaaa gctaaagaaa
    10981 aaataatagc agaatatgaa gaggctgttt tcaaaagact tcaagacaat caattatctc
    11041 cattttcaaa agaagcattt ttagcactag aatatagccc tcgtttgaga gatttgtgct
    11101 ctgataaaat aaaggatgat ctattacgct ttatatactc cccagatgga gtcatttctt
    11161 acaaattatc tgatattgcc tcaacctcat taagagaaaa aacaacgacg cttgatatcc
    11221 aacattgcag cactgatcaa aggaattttt tgtgtaaaaa tcaattttta caagccttta
    11281 ttctatgtca gcaattaggt ttagagggag aaatacaatt cttgcgcaac aaagtgaatg
    11341 aattggcaat acaagttatt aatgatcaaa gtgctttcat tacagggagc aaatatgaat
    11401 tgcaatcagg cttacatcag atgattaacc taaacaatat aatgacttta atagataagg
    11461 acaatcacct aaggggtaac attgaatcaa tcttattgga gatagtttct aaactgatag
    11521 acacttacac aaatgaaagg ctgaatttat acgaaagcta catgttactt aagacactcg
    11581 aaaatgcagg cattccagat gtcaaattaa aacactactt taggcctgta ttaaacaaag
    11641 cgaatgagac cctccatgag aggaaacccc atgattgggg tcattattgc tccattgcca
    11701 ggttggtagg agatgaagcg aaaccttata tttacagctg gttgaatgat tacgacaagc
    11761 cattctattc cgagattaat tcgatgcttg agacatttaa gaatgccggg attgtttttg
    11821 atgatgaaaa tttggatgta ttcaaatcat tattggaaaa aattcattac aatgatgtgg
    11881 cctacaacta cgttcgctta tcatattggc ttagggtgat gaataacctg cagtccttag
    11941 cttctgaaag ccattattca ggatcccaac tggaaatttt aaaaaaccaa tttgatagag
    12001 tcattgaaaa gcttatcaag actacaaaat cagagtatga acatgcttcc tactactcac
    12061 aagaacaaaa atgttaccgt caattaacat cgtttgctga agaaattaac accattttgg
    12121 cctctgattt ttgtttacca gtccctgact cgctgattac ggaattcaat ctgactctga
    12181 ttgcctttag tgaaatggac ttaagccaaa ctaaagtgga tgaatcaaag tttcatcttt
    12241 taaaacaact tcataataaa ttgccaaatc cggaaaaaag agagcaggcg atggaaaagc
    12301 tcactgaatc tatggataca aaaaaacaag ttgaaaaaga tcaaaataaa aacatttcag
    12361 cagcgggcct ctagcgtgat aatatcagac taattagatc attatttaat ctacttgagc
    12421 caataaattt catattaatg cgttgagcga tgttaacatc ttttggtttg taaaa
//
LOCUS       DACTJE010000092        12468 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.092, whole genome shotgun sequence.
ACCESSION   DACTJE010000092 DACTJE010000000
VERSION     DACTJE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12468
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.092"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            177..1256
                     /locus_tag="I9760_001912"
     CDS             177..1256
                     /locus_tag="I9760_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="HAT5043676.1"
                     /translation="MKSKSINLCFFLFILYFGSPILLAQPSRVDNIINELVQPIIETH
                     RTPGLAIAIYYNGKDYYYNFGFADRKSKKPVTKNTIFELASITKIFISTLVALEVQKG
                     KLNVTDYAVQYTPELSKTNGLPIDRVQIVHLATHTASFPKQMEQFGVNKGNIKAFFAR
                     LKTWRPPVKIGTQYKYSNIGFGYLGYVLENASNETLPNLLKSNITKPLGMSHTFFNVP
                     ENLANYEAQGYRPKNKPAPYYRPANFLGGGALRSSAADMLVFLKANLGVQPGTASPEL
                     LSAMQYAQQPFFEVNPNFVMGLGWQRVKRGRHIVITKNGANQGFSTFIGFSPAKKLGV
                     VVLINQARGKATWLGNQILNSLLRL"
     gene            1451..1888
                     /locus_tag="I9760_001913"
     CDS             1451..1888
                     /locus_tag="I9760_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043677.1"
                     /translation="MKKSSPVYSLLLLSAITFSPVIKASPIGMMVDIYCPITQGGPNI
                     ITNFGDYIGGYGMENILSQNNPIYFKSISLAHDVPAQLGNYYNETTSYNSTTGQVTCS
                     YISNNPTEPRFAVAYTLTNGRGGSVQWQSGNSINIIFPVGLKS"
     gene            1909..2070
                     /locus_tag="I9760_001914"
     CDS             1909..2070
                     /locus_tag="I9760_001914"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043678.1"
                     /translation="MPDCLLRLPATLSGIDLLGPYNQSQTKQKNIPDNWGIQAETHDL
                     YDFNSGQIS"
     gene            complement(2088..2381)
                     /locus_tag="I9760_001915"
     CDS             complement(2088..2381)
                     /locus_tag="I9760_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043679.1"
                     /translation="MNVNSENTQSGEALTTEDYSKAMNFIGQNLLSSLTQSVEKLPPQ
                     LRNRRLVCQALSAFLTNVIYKQFPEQPESCQQMLDDITKHVSMQLDKIPQPSK"
     gene            complement(2530..2721)
                     /locus_tag="I9760_001916"
     CDS             complement(2530..2721)
                     /locus_tag="I9760_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAT5043680.1"
                     /translation="MLYWALIFLIVAIVAGLFGFRGVASAATGIAKVLFFLFIVMFIV
                     LLVFSLLGGTPEPVVIVKP"
     gene            3016..4122
                     /gene="legA1"
                     /locus_tag="I9760_001917"
     CDS             3016..4122
                     /gene="legA1"
                     /locus_tag="I9760_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegA1"
                     /protein_id="HAT5043681.1"
                     /translation="MCNLTIHNIENYENDPQLRLIPWILWENLFQHFISANELSLMTL
                     SYKEAIHIFLPGTKNMEQVRQLLCLYYAHYNRNAKQLWSDAHKKGIKSEVICFVAAIT
                     GCSSALDTLCLLLTSDEIVKVIQAENYQAFRLAAENGHLHVLNRLCELAPTEIMAMIQ
                     AENYHAFRLAAENGHLHVLNRLCELAPTEATAMIQAENYYAFRWAAVGRGHHNVINFL
                     LDCPVMLAYAEIHEFEYGEKYVNPFIARHVNRLKEMHDAFKLSNPDGVFDLVTKSECL
                     QGFYMLRNLIRRNDEVLLDDIRFLLSIPGIKALAPTATIPGDANELLRLALRLGNQGA
                     CALLLSIPSVLALTKANNYYINETGGRLDLRAVA"
     gene            4233..5078
                     /locus_tag="I9760_001918"
     CDS             4233..5078
                     /locus_tag="I9760_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HAT5043682.1"
                     /translation="MIKDVFSSSFYFLFFYFMIIYSLDCKYEFSIIVNKKRATVYTCI
                     RFGITSIVLLAICSVPSYGTPIFRDHSLTLSGLIGYTDFKDPVDQQLVISHYVTDLLT
                     NSHKHTHPSVLISAKQQLNFEANKIHKIMLGPALYFQKLHYSGDVWELMLPEFYNYQY
                     DYTSNNVNFLIEGDLYFNPISTWLISFVTAGLGYGVAYIHYDDYALPGIDPVSERHHF
                     DSSIKGIYQLGAGLAVPVNPNWSINLRYAYIHMGKVHTSPNSLGPIELVLNNQNILIG
                     INYSI"
     gene            5091..6806
                     /locus_tag="I9760_001919"
     CDS             5091..6806
                     /locus_tag="I9760_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAT5043683.1"
                     /translation="MKKTGYLLMFVLVACLCNQKIVAAIPLVELIAKPNPSQFVKTQS
                     SVLLFYTLRNNTSVGFPLSYLFSSNNASLSNAGNTCGTFIGPNKTCQFTVQYQAPAIS
                     ITEKLIVQVAYQGRAPVSDIVSINVDNNIACTLLNTASYQTPFCQEQYQKVLQFTPGV
                     FNTTNQNVQEEQTLGGIFGLYRKTGGTEQICYISCGLRSLNGAPPNENTVFELASVTK
                     TFTGSVLGKFIINGTIVSQFDEIRNYPHPAGLSFNINEETVSFQQLATFSGGVCFSNA
                     PSVNQSSTDQAVNQMNFITDINALNPDPNSGNCSNGAPNVSPVYATPPFLPTHNFYSN
                     SSVGLLGQSLIRIDGFANVLETDFNNWICKNVANPLDMIRTSACLPDEAHNGTCGAAA
                     AYCAYTANWITAEYASGYHLFQNSYQLGNPFPFLPWAPAGGIRSNASDMIKFIRANLG
                     FNTGVHNPEQAQLIQGMLMAHEPNIYFPGGTEPNIGNQSPIRGGQGYAWVCMPSPTNG
                     DRICGKIGGHKNFRSFLGLNKSKNYGLIILFNTGALTTDGSLTPATTPPTISQIGTNL
                     LENID"
     gene            complement(6936..7730)
                     /locus_tag="I9760_001920"
     CDS             complement(6936..7730)
                     /locus_tag="I9760_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HAT5043684.1"
                     /translation="MNKKLSLIALCISSLSLHAGTMGEVVSPAYPWFASIGTGYSWTE
                     KPGIVNPNPLFWDFSLQGYDSDLGDRGFYTFAVGKQIHQYIDISLSYLDHEVFNYQKF
                     QTSLPGTSGTPGFTGRSRTRYFELDNKALLVNGYLHPAQAWTSLASIALTPFIDGGIG
                     YARNEVRNFYTVGTTTVVGVAIGSTSSIGEPVNKNSFAWQASAGVNFHPTGSHLSIDA
                     GYRYYDGGQFNGPSVVYTNSDGFQTGTPWSGRLKANQVFVDFKYTV"
     gene            7925..8476
                     /locus_tag="I9760_001921"
     CDS             7925..8476
                     /locus_tag="I9760_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5043685.1"
                     /translation="MKKDITLFTSARPELKFFRLSECKEHLDTAAMWAKNAWGYMHLD
                     DEQFHYKQLQELCEHVYIAMYDGLPVGMLAVIEKTLSLEENREESSEKIVEEPSTPEI
                     TEMKNAYLDYVYIDERARSIGLAGIIIEKAKNLAREMNAEVMSLETLNSTLNGFYKRK
                     GGRVICEGRYNDSYPTEVMRLRF"
     gene            complement(8571..10832)
                     /gene="lem25"
                     /locus_tag="I9760_001922"
     CDS             complement(8571..10832)
                     /gene="lem25"
                     /locus_tag="I9760_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem25"
                     /protein_id="HAT5043686.1"
                     /translation="MSTSEKDVREQKVKTVTLSFLGTGQHREKVHHILTSFHNTISEV
                     NKDNPTVAMRMFDGPGSEPKSGDSKDPIPGTYIYNPKDNSKILISPVISQTITNAIQK
                     LTGNLAGEGIEHLLFEAVLYLNDIIEKNGGKLPETVNLHGFSRGADTCMRMANLLYQL
                     YPDIKVNLFLIDQVPGPGKRDDPHSYTVPPNVEHFESTLMLHEYRPGFDPQHSGRYVI
                     ADPEKTKVVVKPYYGEHNTGNRVTEDPNTNHTAILLNDDMNRFCRETGSLPSVGISPP
                     IIARVGDKKEEVRTHSELSPEKRFELLCGMKENEWGYAKLTKKYHERSILSKREDYVQ
                     DSRLFVNQEHRELFKQLYPKSFNWFFEKNHGGQTKKEEVIVELKSLSEDPRYEHFFSS
                     LAKHFQINENNIAGTLPEPSGIDRDEKSSFGQPPVRDRLSYLQHSLTSIANYYHYHCD
                     EKSSTNESVKNLLLERVKESRTKPDSEAIKHLEQTMDEVRQILESKNEKGFLWQQINH
                     ISPNARQYCEQVKAALREHLEHNQVLSDTQKEEIRKAMDRMDNIVNDSSKDSQQKYRE
                     IRREVIELNAKATTPEDDNQLTRSHFQKAYFELSGDTQKTLNLESLSQTLNQLSKAHY
                     GETSMTDKITQRLDGYKNRNWFWNSVKEVLNFFNIPLPKLHSEVKEQIADKLKERLVD
                     LKEKGMGNDVNAITRELGKAREDLIEHYKKTSKLEMGELDKIINKSMEELLVARKVTK
                     DLVHEEVSQVKLN"
     gene            11157..>12468
                     /locus_tag="I9760_001923"
     CDS             11157..>12468
                     /locus_tag="I9760_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043687.1"
                     /translation="MKIILSILIALHATSTYAGQQVLVLSGGDNPGLNHYTQYLQTKT
                     LYEHLITRFNPETVSIYFGGGNTPTTQLSPFDVHKLINTKDNQQNKEDVMFTGIIANN
                     QQANKYNVDAFFLSSKIQQINPGDNLLLFVSDHGMPHGFLEDKNINPYSNNCIDLWHY
                     QKPFINNFTSAKNFSKACLSKNELSSLLTLIPSQHVIFAMSQCYSGGFHQLSVTTQDG
                     YPVANTKICGFTSSTDDHYASGCTADANGATYQGYERSFTEWYTGHGVIDGKKIREPA
                     KSMLVAHQNAIIDDMTVDIPLSTSDYYLLQWAKLFASKQFKSRTKHYDNNMIQSIYLN
                     YKKRLQSSNNDALSQFMHVARASEDKIVQLMPEAGEFASLSITQQKQKISSLEKQLER
                     QGKELQAVWEGMMNLTLNVITPTWEKTVNHSKNQPLNEKQQTFEK"
BASE COUNT         3903 a         2477 c         2245 g         3843 t
ORIGIN      
        1 cttttctaaa aaagatcgat aattaatcaa gaatgctgag actaacaatc tgtagaacgc
       61 aaacacggtt tttgctttgg agcccttaca caatcaggat agaatcgcta caagatgtcc
      121 tgacgatgca aaataaagaa aaaataatta ttatattttc ccaaaagaaa ttaatcatga
      181 aatcaaaatc cattaattta tgcttttttt tattcattct atactttggc agtccaattc
      241 ttttggcaca accgtccagg gtggataata taattaatga actggttcaa ccaattattg
      301 agacacatcg gactcctggc ttagccattg ccatttatta taatggaaag gattattatt
      361 ataattttgg atttgctgac cggaaaagta agaaacccgt cactaaaaat actatttttg
      421 aattggcatc gattaccaaa atattcatta gcactcttgt agccctggaa gttcagaaag
      481 gaaaattgaa tgtaacagat tatgccgttc aatatacacc tgaactttca aaaaccaatg
      541 gactaccaat tgacagggtt caaattgtgc atctggcgac ccacacagca agctttccca
      601 aacaaatgga gcaatttggt gttaataaag gcaatataaa ggctttcttt gcccgtctta
      661 aaacatggcg tcctccagta aaaataggaa ctcaatataa atattccaat attggttttg
      721 ggtatttggg atacgtattg gaaaacgcct caaacgaaac tttacctaat ctgttaaaat
      781 cgaatattac caagccattg ggaatgtcgc atactttttt taatgtacca gaaaacctcg
      841 ctaactatga agctcaaggt tatcgaccta aaaataagcc agcaccttat tatagacctg
      901 ccaattttct aggcggaggt gctttgcgct cttctgcagc tgacatgctg gtatttttaa
      961 aagcaaattt aggagttcaa cctggcaccg catcaccaga attactttct gcgatgcagt
     1021 atgcccaaca accatttttt gaagtcaacc caaattttgt tatgggatta ggttggcaaa
     1081 gagtgaaaag agggaggcac attgtgatca ctaaaaatgg agcaaatcag ggatttagca
     1141 cctttattgg tttttctcct gcgaaaaaac tgggagtcgt cgtattaatc aatcaggcaa
     1201 ggggcaaggc aacctggctg ggaaaccaaa ttcttaatag tttgcttagg ttatagctta
     1261 aaggactatt tttaagcata gactcatggg cattgacaat caaaatgtcg tgcaacatga
     1321 ttatccttga ccctattctc tgtgcgtctt gacttacaat atggatagta tgattttaat
     1381 ctaaactaat gacgaacaga gcctcaggtc aaaccaacaa cttatcaaat aactgaaaaa
     1441 ggaagtttca atgaaaaaat ccagtcctgt atactcgttg ttattgcttt ctgctattac
     1501 cttttcgcct gttatcaagg caagtcctat cggtatgatg gtggatatct attgccctat
     1561 tacccaggga gggccaaata tcatcactaa ttttggagat tacattggtg gttatggcat
     1621 ggaaaatatt ttgtctcaaa ataatcctat ttattttaaa tccatttcgc tagcccatga
     1681 tgttccagct caacttggaa attattacaa tgaaacgacc agctacaaca gcacaacagg
     1741 tcaagtcacc tgcagttaca taagcaataa cccgacagaa cctcgctttg ctgtggcgta
     1801 taccttaacc aatggcaggg gcggttctgt ccaatggcag tcagggaatt caatcaatat
     1861 catttttcca gttggtctaa aaagttaacc aaagtttact ggattaaaat gcctgactgt
     1921 cttcttcgcc tcccagcaac tctttctgga attgatttgc tgggacccta caatcaaagc
     1981 caaaccaagc agaagaacat acctgataat tggggtattc aagctgaaac gcatgattta
     2041 tatgatttca actcgggcca aattagctga tacagattac tagcgtttta ctttgatggt
     2101 tgagggattt tatccagttg catactgaca tgcttagtaa tatcatcaag catttgttgg
     2161 catgactcag gttgctctgg aaactgttta taaataacat tagtcaaaaa tgcagacaaa
     2221 gcctgacaaa ctaacctgcg attacgaagc tgtggaggca acttttctac agattgagtt
     2281 aatgatgaca ataaattttg gcctataaaa ttcattgcct tggaataatc ttcagtagtt
     2341 agagcttcgc ctgattgagt gttttcacta ttcacattca ttttttatcc tgtcagtgtg
     2401 agagcaagcc agattataga gtctatctca agctacttca acgactgttt caattctttc
     2461 cgtaaaagca gcttgattat caattaaaac aacaaaatac atcattaaga ggacttatat
     2521 aggatgtagc tatggtttta caatgacaac aggctctggt gtgccaccca aaagactaaa
     2581 gaccaaaagc acaataaaca ttacgataaa gaggaagaac agaactttag ctatccctgt
     2641 tgcggctgat gctacccctc taaatccaaa taaacctgcg acaattgcaa caataagaaa
     2701 aattaaggcc caatataaca tgataaactc cctgtctttc agaataataa gttcttcctg
     2761 aattgttttt accagagcaa aatcagatcc atctgttttt gctcatctca cttataaaaa
     2821 cgaaagaaat tgaaatactt atttatagtc atcctactaa aattgtagac tatttatcca
     2881 aaatatagaa aaacaaacaa cttttaggat atttcatatt gtcctctttt aaccgtagat
     2941 tgtccttatt cgaccatcca gctcaattgt gattaattta taattgttta gtttgttttt
     3001 tatctattta gatccatgtg taatctaact attcataata tagaaaacta tgagaacgat
     3061 ccgcagttac gattaatacc ctggattcta tgggaaaatt tatttcagca ttttatcagt
     3121 gccaatgaat tatctcttat gactttatca tataaagaag caatccatat ctttttgcca
     3181 ggaacaaaaa atatggaaca ggtgaggcaa ttgttgtgtt tatactatgc ccattataac
     3241 aggaatgcca aacaactctg gagtgatgct cacaagaaag gaataaaaag cgaggttatt
     3301 tgttttgttg ctgcaataac cggttgttca agtgccttag atacattatg tcttctttta
     3361 acatctgatg agatcgtgaa agtgatccag gcagaaaatt accaagcttt tcgtctggcc
     3421 gcagagaatg gacacctcca tgtgctaaat cgtttatgtg aattggcgcc aactgaaatc
     3481 atggcgatga tccaggcaga aaattaccat gcatttcgtc tggctgcaga aaatggacac
     3541 ttgcatgtgc taaatcgttt atgtgaattg gcaccaactg aagctacagc aatgattcag
     3601 gcagaaaatt attatgcttt tcgctgggca gcagttggaa gaggccatca taatgtcatt
     3661 aattttttgc tcgactgtcc ggttatgctc gcttacgcag aaatacacga atttgaatat
     3721 ggagagaagt acgtcaatcc gtttattgcg aggcatgtga acaggttaaa agaaatgcat
     3781 gatgccttta agctaagtaa cccggatggt gtctttgatt tagtcacaaa gtcggaatgc
     3841 ctgcaaggtt tttatatgtt acgcaattta atccgtcgta atgatgaagt attactcgat
     3901 gatattcgtt ttttgctgag catccccggt ataaaagcct tggctcctac tgcaaccatt
     3961 ccaggagatg cgaatgagtt actgcgtctg gctctacgat taggtaatca aggggcttgc
     4021 gcattactat tatccattcc atctgtacta gcattgacta aggcaaacaa ttattacatt
     4081 aatgaaacag ggggacgact cgacttgcgt gcagtagcct aaagttcttt gaaagatgtt
     4141 tcaattgatt ttatttaata ctttataaaa atcctgggct acaagaagaa tacttgttgt
     4201 gactgataga gtattaatac tgaattcttg atatgataaa agatgtcttt tcctcttcat
     4261 tttacttcct atttttttat ttcatgataa tttattctct tgattgtaag tatgaatttt
     4321 caattattgt gaacaaaaaa agggcgactg tgtatacatg cattagattt ggaataacct
     4381 cgatagttct tcttgcaata tgttcggtcc cttcttatgg cacaccaata tttcgtgatc
     4441 atagcctgac tctatcaggc cttattggtt atacggattt caaagatcct gtcgatcaac
     4501 agttggtaat cagtcactat gtcactgatt tactgactaa ttctcataaa catactcacc
     4561 caagcgtatt aatttctgcc aagcaacaat tgaattttga agccaataag attcataaaa
     4621 tcatgctggg tcctgcattg tattttcaaa aactgcatta ttctggcgat gtgtgggaat
     4681 taatgttacc tgagttctat aattaccaat atgattatac aagtaataat gttaactttt
     4741 taatagaagg agacctatat tttaatccta tatctacttg gcttatttct tttgtcactg
     4801 ctggtttagg atatggggtt gcttatattc actatgacga ttatgccctt cctggtattg
     4861 atcctgtaag tgagcgacat cactttgact cttctatcaa aggaatttat caattgggag
     4921 caggccttgc ggtaccagtc aatcctaatt ggtcaattaa cttacgctat gcctatattc
     4981 atatgggcaa agtccatact tctcctaact cccttgggcc tatagaactt gttttaaaca
     5041 atcaaaacat actgatagga attaattact cgatataact caggccaaat atgaaaaaga
     5101 ccggttattt attaatgttt gtattagttg catgcttgtg taatcagaaa atagtggcag
     5161 ccatccccct ggttgagttg attgcaaagc ccaacccctc tcaatttgta aaaactcaaa
     5221 gttctgtctt gttattttat actttgcgca ataatacaag tgttggcttt ccgcttagct
     5281 atttattttc cagcaataac gccagcctat caaatgctgg aaacacctgt ggaacattta
     5341 ttggtccaaa caaaacatgt caatttactg tacaatatca agctccagct attagtatca
     5401 cagaaaaatt aatagtccaa gtagcttatc agggacgggc gccagtaagt gatattgtct
     5461 ctatcaatgt agataataac atcgcctgta ccttactcaa tacggcatcc tatcaaactc
     5521 ctttctgcca agaacaatac caaaaagtac ttcaatttac tcccggtgta ttcaatacaa
     5581 caaatcaaaa tgttcaggaa gaacaaactt taggagggat atttggcctg tatcggaaga
     5641 caggaggcac tgaacagatc tgctacataa gttgtggatt aaggtctcta aatggtgcac
     5701 cacccaatga aaacacagta tttgaattag cctccgtcac caaaactttt accgggtcgg
     5761 tattagggaa atttattatc aatggcacta ttgtttccca atttgatgaa attcggaatt
     5821 atcctcatcc cgcaggtctt agcttcaata tcaatgaaga aactgtgagt tttcaacaat
     5881 tggctacttt ctccggagga gtttgtttct ccaatgctcc cagtgtcaat caaagcagca
     5941 cagatcaagc cgtcaaccaa atgaatttca taacagacat caatgcgcta aatcctgatc
     6001 cgaatagcgg caattgtagt aatggtgcac ccaatgtaag tcctgtatac gccactccgc
     6061 catttttacc aacacataat ttctactcaa acagtagcgt gggtttacta ggacaaagtc
     6121 ttatccgtat cgatggtttt gctaatgtcc ttgaaaccga cttcaataat tggatatgta
     6181 agaatgtcgc caatcctctg gatatgataa gaacgagtgc ttgtcttcct gatgaggctc
     6241 acaatggcac ttgtggtgca gcagctgcat actgtgctta tactgcaaat tggataacag
     6301 ctgaatacgc ttcaggatat catctatttc aaaacagtta tcagttgggc aatcctttcc
     6361 catttttacc ctgggcacct gctggaggaa ttcgaagtaa tgcctctgat atgattaaat
     6421 tcatacgcgc taatttgggc tttaacacag gggttcataa ccccgaacaa gcccaactaa
     6481 ttcaaggcat gttgatggcc catgaaccaa atatttactt tccaggtggc acagagccta
     6541 atataggcaa ccagagcccc atacgtggtg gccaaggcta tgcctgggta tgcatgcctt
     6601 ctccgactaa tggagacagg atctgtggaa aaattggcgg acataaaaat tttcgcagct
     6661 ttcttggctt aaataaaagt aaaaattatg gtttgattat tttatttaat acaggcgctc
     6721 ttacgacaga tggaagtcta actcctgcga caacaccacc gactataagt cagattggca
     6781 ctaatttatt agagaatatt gattaatata ttctgcttcg atagatagga aacgggagtg
     6841 taaccattca tcattccttt acctgctatg tgtgaactac tgcataaacc atgtagctcg
     6901 acaatgcagt aatccaattt tcctgattgg aataattaca ctgtatactt aaagtcgaca
     6961 aaaacctggt ttgccttcaa acgacccgac cagggtgttc ctgtctgaaa accatcgctg
     7021 ttggtataga caactgatgg tccattaaat tgaccgccgt cataataacg ataacccgca
     7081 tcgatactca gatggcttcc tgttggatga aaattcaccc cggcagacgc ttgccacgca
     7141 aaactgtttt tgttaactgg ctcaccaata cttgaagtgg aaccaatggc aacaccaacc
     7201 acagttgttg tccctacggt ataaaaattg cgtacttcat tacgcgcata accgataccg
     7261 ccatctatga atggggtcaa agcaatactc gccagactag tccacgcctg ggctggatgt
     7321 aagtagccat ttactaataa tgctttatta tcaagttcaa aatagcgagt tcttgacctt
     7381 cctgtaaaac caggggtacc agaggtgcca ggtagtgacg tttggaattt ttgatagtta
     7441 aaaacctcat ggtcaagata acttaggcta atatcaatgt actgatgaat ttgtttccca
     7501 acagcaaagg tataaaatcc cctatccccc agatcagaat cataaccttg taatgaaaaa
     7561 tcccaaaaaa gaggattagg attaacaatc cctggctttt cagtccagga atagccagtg
     7621 cctatagaag caaaccatgg ataggcaggt gaaacaactt ctcccattgt tcccgcatgc
     7681 aaagaaagcg aagaaataca taaagcaatt agactcaact ttttattcac gataatatcc
     7741 ttatttaaat gggtaataac agacttactg ttatgaagcg cctttgaaaa cggcgtttag
     7801 tttaaatgaa caagaaaaat tagcaagctt gccaaactac tatcttgggt tgataaagtg
     7861 cagataaaca gtatcatata gcattttttc catttgctaa ttcatagtcg caagaggtac
     7921 aagtttgaaa aaagacatca ctttattcac atcagcaaga ccagaactaa aatttttcag
     7981 attatccgag tgcaaggaac atctggatac tgcagccatg tgggcaaaga atgcctgggg
     8041 atacatgcat ctggatgatg aacaatttca ttacaaacaa ctgcaggaat tgtgtgaaca
     8101 tgtttatatc gcgatgtatg acggactacc ggttggaatg ctcgctgtca ttgaaaaaac
     8161 actctctctt gaagaaaaca gagaggaatc ctccgaaaaa attgtggaag aaccctcaac
     8221 cccagaaatc actgaaatga aaaatgctta tttggactat gtgtacattg atgaacgcgc
     8281 ccgaagcata gggctagctg gaataattat tgaaaaagca aaaaaccttg caagagaaat
     8341 gaatgctgaa gtaatgtctc ttgaaacact gaattccacc ctcaatggat tctataaaag
     8401 aaaaggcgga cgagtcattt gtgaaggcag atataacgat agttatccta cagaagtgat
     8461 gagattaagg ttttaatcat atcagcaatt ttgtctgcag cttggagcct gattcatatt
     8521 attgtcaaac taaaaacctg cttatgataa ctctcagaat ggaacagagt ttagttcaat
     8581 ttaacctgac taacctcctc atggaccaag tctttggtga ctttgcgtgc cacaaggagt
     8641 tcttccattg atttattgat aatcttatcc aattctccca tttccagttt tgaagttttc
     8701 ttataatgtt caattaaatc ttcgcgagct ttaccaagct ctctggtgat tgcattcaca
     8761 tcattaccca tgcccttttc tttgagatca acaagtcttt ctttgagctt gtcagcaatt
     8821 tgctctttaa cctccgaatg cagtttggga agaggaatat tgaaaaaatt caaaacttcc
     8881 ttgactgaat tccaaaacca atttcgattt ttatacccat ccaatcgctg agtaattttg
     8941 tccgtcatac tggtttcacc ataatgagct ttcgatagtt gattcaatgt ttgagacaga
     9001 gattctaaat tcagagtttt ttgtgtatcc cctgataatt caaaataagc tttttggaaa
     9061 tgacttcttg ttaattgatt atcatcttcc ggtgtggtgg ctttggcatt taactcaatc
     9121 acctctcttc ttatttccct atatttctgt tgactgtctt tgctactatc attgactata
     9181 ttatccattc tatccatagc cttacgaatt tcctcttttt gagtatctga tagtacctga
     9241 ttatgctcaa ggtgttcccg gagtgctgct ttcacttgtt cacaatattg tcgagcattg
     9301 ggggaaatat gattaatctg ttgccagagg aaaccttttt catttttgga ctcgagtatc
     9361 tgtcttacct cgtccatggt ctgttccaaa tgtttgattg cttcgctatc cggttttgtc
     9421 cttgactctt taacacgttc taacagcaaa tttttaaccg attcattagt tgagctcttc
     9481 tcatcacaat gatagtgata ataattcgca atggaggtca gcgaatgttg taaataagaa
     9541 agcctgtccc tgactggggg ttgtccgaag ctactttttt catccctatc aatacctgaa
     9601 ggttctggca atgttcctgc aatattattc tcattaattt gaaagtgttt cgctaatgat
     9661 gaaaaaaaat gctcatacct tggatcttcg gataatgatt ttaattcaac gatgacttct
     9721 tccttttttg tttgaccacc atgatttttt tcaaaaaacc aattaaatga tttgggatac
     9781 aattgtttaa acaattccct gtgttcctgg ttgacaaaca atctggagtc ttgtacgtaa
     9841 tcttcccgct tcgacagaat actgcgctca tgatattttt tggtcagttt ggcatagccc
     9901 cattcgtttt ccttcatacc acataacaac tcaaagcgtt tttctggaga taattctgaa
     9961 tgagttctaa cctcctcttt tttatctcct actctggcaa taatgggtgg agaaatccca
    10021 acagaaggga gtgagccggt ttccctgcaa aagcgattca tatcatcatt taataaaata
    10081 gcggtgtgat ttgtgttggg atcttcggta acacgattac cggtattgtg ttctccataa
    10141 tacggtttaa caactacttt tgttttttcg ggatctgcaa tcacatacct tccagaatgt
    10201 tgagggtcaa atccaggcct gtattcatgc aacatcaatg tcgactcaaa atgctctaca
    10261 ttaggaggaa cagtataaga atgaggatca tcccgtttcc caggtccggg aacctgatca
    10321 ataagaaata aattgacctt gatgtcggga tataattgat aaagaagatt cgccatgcgc
    10381 atacaagtat cagcccctcg gctaaaccca tgcaaattca ctgtttcagg cagtttgcct
    10441 ccattttttt caatgatgtc attaaggtaa agtactgcct caaataataa atgctctata
    10501 ccttctcccg ccaaatttcc tgtcagtttt tgtatggcat tggtaatcgt ttgggagatt
    10561 acaggactaa ttaatatctt ggaattatct tttgggttat aaatataagt cccaggaata
    10621 ggatccttgc tgtcgcctga ttttggttct gaaccaggac catcaaacat tctcattgcc
    10681 actgtaggat tgtctttatt tacttccgag attgtattat gaaaactcgt gagaatatga
    10741 tgaacctttt cacgatgttg tcctgtaccc agaaaactta atgtgactgt ttttactttt
    10801 tgttcacgaa cgtctttctc tgatgtgctc ataacaggaa tatcaactaa taatggtcct
    10861 tgatataatt ttaaaccatg cgcacaataa atgctccata attgcttaaa atgaatgtct
    10921 ttcttaaaaa tagacttatt taaaacctgt attgaactcg aacagagagt aaggttagag
    10981 aagggtaatt aaaaaacaag gagtagcagt gctgatagct gactatatct tgcctatctc
    11041 taattagtct tagtctttaa tcgtaacgtt tcaattatcg ttagcagttc aatacaagaa
    11101 ttgctatcct tgacacaaca atgaatagaa ttaaaacaac gattcatgga atgaagatga
    11161 aaataattct cagcatttta attgctctgc acgctacgtc tacttatgct ggccagcaag
    11221 ttttagtcct gtcaggagga gataacccag gccttaacca ctacacccaa tacctgcaaa
    11281 caaaaactct ttatgagcat ttaatcaccc gatttaatcc cgaaaccgta agcatctatt
    11341 ttggaggagg taacacaccg accacacaac tttctccatt tgatgtgcac aaactcatta
    11401 atacaaaaga taatcaacaa aacaaagaag acgtcatgtt tacgggtatt atcgcaaaca
    11461 atcaacaagc caataaatac aatgtggatg cctttttttt atcttccaaa attcaacaaa
    11521 tcaaccctgg tgataatttg cttctctttg tcagcgatca tggcatgcct catgggtttt
    11581 tagaggataa aaatatcaat ccctacagta acaattgcat tgatctctgg cattatcaaa
    11641 aaccatttat taataacttc actagcgcta aaaactttag caaagcctgt ttatccaaaa
    11701 atgaattatc atcgctgctc actttaatac caagccaaca cgttattttt gcgatgtctc
    11761 agtgttactc aggtgggttt catcaattat ctgttacaac acaagatggc tatcctgtag
    11821 ctaacactaa aatttgtggt ttcacttcct caacagatga tcactatgcc agtggctgca
    11881 ctgctgatgc caacggcgct acttatcaag gatatgaacg ttcttttacc gaatggtaca
    11941 cagggcatgg cgtcattgat ggaaaaaaaa tcagggaacc ggctaaaagt atgttggttg
    12001 cccatcaaaa cgcgatcatt gatgacatga cagtagacat tccattaagt acttcggact
    12061 attatttact ccaatgggcc aaattatttg catcaaaaca atttaagtcc cgcacgaaac
    12121 attacgacaa caatatgatt caatccattt acctgaatta taagaaaaga cttcagtcca
    12181 gcaataatga tgctctttcc caatttatgc atgttgcccg tgcttccgaa gataaaatcg
    12241 tacagttaat gcctgaagcc ggtgagtttg cttctctatc cataacacaa caaaaacaaa
    12301 aaatcagttc gctggaaaag caattagagc gacaaggcaa agaattacaa gctgtttggg
    12361 agggaatgat gaatctgaca cttaacgtca tcacgccaac ctgggaaaaa acagtgaatc
    12421 attcaaaaaa tcagcctctc aatgagaagc aacaaacttt tgaaaaag
//
LOCUS       DACTJE010000093        12319 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.093, whole genome shotgun sequence.
ACCESSION   DACTJE010000093 DACTJE010000000
VERSION     DACTJE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12319
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.093"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..2769
                     /locus_tag="I9760_001924"
     CDS             <1..2769
                     /locus_tag="I9760_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAT5043688.1"
                     /translation="DLTFTPRGGGTGTNGQSLNAGIIIDCSRFMNHIVEINSDEDWVL
                     VEAGVVLDQLNEYLKPYNKFFAPALSPSNRATIGGMVNTDASGKGSRLYGKTSQYILA
                     LTAVYLGGEVHTSEEIDVSLLDNMKKLPGQVGQFYKQLDKTVQEHRETIDRQLPKLHR
                     FVSGYNLAKIYSNDGKRFNANYILAGSEGTLAYVTKIKLKLTNIPKYKCLFVVLYPTF
                     SDALEHAKHLVAFDPEAIETMDSTILKLAKTDIIYHSLKSFLSSGNNIQVDAINLIEF
                     VANDEKVLLHHAQLLKKSLKGTDFHFVTNNEDIINLWELRKRSVGLLGKVKGVRKPIS
                     GVEDTVVPPEYLADYVKEFRELLDSHGVSYGMFGHVDVGCLHVRPELNLMDAKDREIY
                     YEIIEKVAQLAHKYGGVLWGEHGKGFRSEFVPVFFGEELYNELRKIKSSCDPFNQLNP
                     GKIAVPLGSHDSLASIRSEKTRADVDSQINYQYRDSYQLPLNCNGNGACFDYKLQDVM
                     CPSYKISRDRIHSPKGRASLVREWLRQLSALTGKRELTWPLKKRLNTYKRNHGQYDFS
                     HEVYTALEGCLGCHACRSQCPVNINVPSFKAKFLQTYHTRYSRSIKDYLIAYSERVGY
                     FQSLFPELANFCLSNKLVKYCLHKYTGLVDLPLFSSPNLKSLLKANNIPWLDLNHLPV
                     SEKTVIYIQDWVTSFYEAELVLKACQLIKKLGFQVYVLPWFENGKTLHDNGMLQSFAK
                     ITRKNDLMLQKLSDHRLTMIGLDPAMVLTYRYEYSEYLHENYTSKILLIQEWLSQILG
                     SEKVNELPIKANEKLNQYLLLSHCTEQANCSEALMQWQKVFQFFGLTLVAEKTGCCGM
                     AGAYGHESIHQFNSHGLFDLSWKNYFEDSTGLANTLVDGYSCRAQVKRLTGLKASHPV
                     EILLGLFE"
     gene            complement(2857..3513)
                     /locus_tag="I9760_001925"
     CDS             complement(2857..3513)
                     /locus_tag="I9760_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043689.1"
                     /translation="MKAVANRYYVLCQEKVFIRSLLSGFFLLFISFLIMNFAGKYASL
                     MAGNPVNDLILDNLPLRDVTLFHVNAAIIFWTIFTIYTLLNPAFIPFITKSAALFILI
                     RCAFISMTHLGAPPNQLSIPDNFSSLVLFDGDLFFSGHVGGPFLMMLIFWNKTTLRYI
                     CLAAAVFFAYTVLIGHIHYSIDVFAAPFITYGIFHIGQFLFASDYQRLKSELTSSTGN
                     "
     gene            complement(3513..4676)
                     /locus_tag="I9760_001926"
     CDS             complement(3513..4676)
                     /locus_tag="I9760_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAT5043690.1"
                     /translation="MKILTVLDSYPPDLNGGAYFTHRLAKFLQAKGHEVLVICPSRSL
                     KQGYTSYEGVNLYGVRSWPALGYKNFRVCWPFFIKKGILKAITDFNPDVVHLQGKFFL
                     GGICYRACRKKGIPLMATNHFMPENFFHYTHLPKYFEKWFHRTTWNIVIDMLNHVKIV
                     TTPTHTAANLLKEVHIQKEIHVVSCGVDLQKFQPKQNANLIRQRYKIPDKPILLYAGR
                     LDKEKNLSIAIKAFYKARQSIDAHFVLTGRGAELQRLKKLVQTLNLTEHVTFTGYLSD
                     AEYPLVYSLANCFVNPGTAELQSIVALEAIASGLPLLAAKAMALPELVKEGVNGYLFD
                     PNDVETLSCYMVKILSDRTFSEQMGRESRKLSQEHDIKRTIEQYETLYQSMVS"
     gene            4826..5809
                     /locus_tag="I9760_001927"
     CDS             4826..5809
                     /locus_tag="I9760_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HAT5043691.1"
                     /translation="MSSAKIIGMGKYLPANIVFSSDLDKKLNLPEGSVQKKSGLVSRH
                     FARVSETTSYMASQAALHAVEQAGITLAEIDAIIGACGAGEQPIPCTAVLVQKQLGLD
                     NSGIACFDINSTCLSFLTALDAASYMLSGGRFNRILIVSSDIPSFGLDWSDLETCTIF
                     GDGAAACVVERSNDYSRILAAHMKTYSFGADFCQVQAGGTFMPPASEYDKKYGLFKMD
                     GKRVFKLASQMIGPMQEELFAKASITLNDVDWVVPHQASLLAMHHIRKKLGIPGEKFV
                     DIYTTHGNQMAASLPSALCHLIHNHNLERGQLVYLLGTGAGLSAAGMILEY"
     gene            5811..6797
                     /locus_tag="I9760_001928"
     CDS             5811..6797
                     /locus_tag="I9760_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAT5043692.1"
                     /translation="MISVVTGATGCLGLNLTRRLIKEGHAVIALGRNLQLGEIISQIG
                     ATFVPLDLKDLESLSLISQNADFIFHCAALSSPWGKYKDFYQANVIGTHNVVQATPSQ
                     ARLVYVSTPSIYFDFTEKHNIKENALLPAKPVNYYVQTKLIAESIVDKAQLQHDLDVI
                     TIRPRGIFGPYDRAIFPRLLKAERQGVLPIIGSGNHLIDITFVENVVESLILAALADK
                     CYSGKKYNITNDEPRTFIDIISRMFSALNKPLKTRSIPYNHARFVAKFLEFLHRLLYL
                     KTEPKITEYGVGVLAFGQTLNIEEAKKDLKYKPIYSIDEGIMKFAEWYCSCI"
     gene            6776..7612
                     /locus_tag="I9760_001929"
     CDS             6776..7612
                     /locus_tag="I9760_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT5043693.1"
                     /translation="MVLFMHIEYQLFEAGYCKHCERMTLKNGKIRQREYPSFCALIKH
                     PSHGYILFDTGYSDRFTCLTQKFPASIYRHLTPMTLVKSLKEQLLEIHIKPEDINYIV
                     ISHFHADHIGGLCDFPNAHFICHSDAIDDIQHTKGLKALLKGFLPGLIPEDFFERLLP
                     LQKTIQLDEHLLPFSDGFDVLGDNSLIAIPLPGHAKGQIGLYFKAPGDIFLVADSCWH
                     QETFKELIYPSALTYLIHHDKRAYLKTIQKLHELHQRNKTIEIIPSHCQQLRSRIGNP
                     LC"
     gene            7606..8880
                     /locus_tag="I9760_001930"
     CDS             7606..8880
                     /locus_tag="I9760_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HAT5043694.1"
                     /translation="MLARILFHYFNTLYLNKTITTPAKLKLHQAKQFKKLIKNCLHQS
                     AFYRDYLKTPLEQWPIVNKKIMMEHFDDVNTVNVKKTEALEIALRAEHTRDFSPMINN
                     IAVGLSSGTSGSRGLFLVSEQERDAWAGIILAKAMPNGLRSRERIAFFLRANNQLYTT
                     LNKSRTIQFHFFDLFADFDTHIQRLNAIQPTIISAPASVLLMLASQQDILKITPKKIF
                     SVAEVLEPNEEEILTRAFGCTISQIYQCTEGFLAISERKTNRLLMNEEFLIIEKEWLD
                     EERFVPIITDLYRSTQPIIRYRLDDVLVTERSNGVFTELKNIEGRLGDVCYGKRGQEI
                     VPIFADVIRQHMISSGVSFEDYLICQQTLTQFTIQISPEISNKNQFIEHLNQLFIYKN
                     CELPQWNWELYDKSQPGVKRRRVRSLYCPKTE"
     gene            complement(8974..9834)
                     /locus_tag="I9760_001931"
     CDS             complement(8974..9834)
                     /locus_tag="I9760_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043695.1"
                     /translation="MKKILITLIGFLFMNTCHHLLYADNSHHHTVKDLSTPSSKLENT
                     PTIQLEVQKIIDKKDKKLVFVKLTDIKSNKPITFNDLTEIHTKKFHLLIIDNHLIDYS
                     HVHPAETATPGIYQFEWQPNFKNATYRAWADLLPTNTNKQEYATADLTSPQTVTRTMN
                     PIHHRTVLESTVNGYQFKLTFNKMPLLAGKPAMGEIDVTDSKGHPVRTLEPIMGAYAH
                     IVGFNEDFKTVIHVHPMGKEPANSTERGGPQLQFHIEPNKAGFIKMFAQVQIDGKVLY
                     APFAFTVKKA"
     gene            10043..12250
                     /locus_tag="I9760_001932"
     CDS             10043..12250
                     /locus_tag="I9760_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAT5043696.1"
                     /translation="MAHNHHEGHSHVHNEHLCCHKKDNAHKPQIISDGTIFYTCPMHP
                     EVRQATPGICPLCGMALEPEAISIGEATNPEYLDMKHRFWVALILTLPVVILEMAGHG
                     FQHFVSTGISNWIQLLFATPVVLWCGWPFFLRGWQSLKTRRLNMFTLIAMGIGVAWIY
                     SVVAVFFPGLFPAAFRSQEGVVSVYFEAAAVIATLVLLGQVLELKAREQTGSAIRALL
                     KLAPENAHRIKEDGSEEEIALNQVEVGDSLRVRPGEKIPVDGEIQEGRSFIDESMVTG
                     EPMPVAKGIGDKVIGATINQTGSFIMKALHVGADTMLARIVQMVSDAQRSRAPIQRVA
                     DTVSAWFVPAVILVAVLSFITWGLLGPQPSLGYGLIAAVSVLIIACPCALGLATPMSI
                     MVGVGKGAQNGVLIKNAEALERMEKVNTLIVDKTGTLTEGHPKLTHIETSEDWDERQA
                     LLLAASLEYHSEHPLASAIVKAAQEMQIAFISVQNFEAPTGRGVVGRVNSHHVAIGNA
                     KLMHEYGSDDTSLFEKADKFRAKGSSVMFMAVDGKTVAFLVVEDPIKSTTAETIHALQ
                     KKGIEIYMLTGDSKKTAEAVAATLGIKNVIAEIMPEDKGRIVSELKGNGLIVAMAGDG
                     VNDAPALAKADVGIAMGTGTDVAIESAGVTLLHGDLSGIAKARYLSESTMNNIRQNLF
                     FAFIYNMLGVPLAAGIFYPLTGLLLNPVIAAGAMALSSVSVIVNALRLRRVTL"
BASE COUNT         3823 a         2168 c         2546 g         3782 t
ORIGIN      
        1 gatttaactt tcacccccag gggtggcggc actggaacta atggccaatc actaaatgct
       61 ggaataatca tcgattgttc tcgtttcatg aatcatattg tggagataaa ctctgatgaa
      121 gactgggttt tggttgaagc tggagtagtt cttgatcaat taaatgaata cctgaagcct
      181 tataacaaat tctttgcacc tgctttatcc ccaagtaatc gcgctaccat tggaggtatg
      241 gttaatacag atgccagtgg caaaggctca agattgtatg gaaaaaccag tcaatacata
      301 cttgcattga cagctgtcta tcttggagga gaggtccata ccagtgaaga gatagatgtc
      361 agtcttcttg ataatatgaa aaaattgcct gggcaggtag ggcagtttta taagcaattg
      421 gataaaactg tccaggaaca tcgagagaca attgataggc aattacctaa attgcatcga
      481 tttgtctccg gttacaattt ggcaaaaatt tactcaaatg atggcaagag attcaatgcc
      541 aattatattc tcgcaggttc tgaaggaact ttggcctatg tcactaaaat caagttaaaa
      601 ttaacgaata tacccaaata caaatgcctg tttgttgttt tgtatcctac atttagtgat
      661 gctcttgagc atgcaaagca tttggtggca tttgatccag aagccattga aacaatggat
      721 agcactattt tgaaattggc caagacagat attatttatc attctctgaa atcttttctg
      781 agctctggaa acaacatcca agttgatgca attaatttga ttgaatttgt ggcaaacgat
      841 gaaaaagtgt tactacacca tgcgcaatta ttgaagaaaa gtttaaaagg tacggatttt
      901 cattttgtta ctaataatga ggatattatc aatctttggg agttaagaaa gcgcagtgtt
      961 gggcttttag gtaaagtaaa gggtgtaaga aaacccatat cgggtgttga ggatactgtt
     1021 gttcctccag agtatttggc tgactatgtc aaggaatttc gtgagttact tgattctcat
     1081 ggggttagtt atgggatgtt tggtcatgtt gatgtagggt gtttacatgt tcgccctgaa
     1141 ttgaacttga tggatgcaaa agatagggaa atctattacg aaataataga aaaggtggcg
     1201 caacttgccc ataaatacgg tggggtattg tggggcgagc acggtaaagg atttcgaagt
     1261 gaattcgtgc ctgtattttt tggagaggaa ctctacaatg agctcaggaa aattaaatca
     1321 agctgtgatc ctttcaacca attaaatcct ggaaaaattg ctgtcccctt aggtagccat
     1381 gattcacttg ccagcatccg ttcggaaaaa accagagcag atgtagacag tcaaattaac
     1441 taccagtata gagattctta tcaattacct ttaaactgca atggaaatgg agcgtgtttc
     1501 gattataaat tacaagatgt gatgtgccct tcttataaaa taagcaggga tagaattcat
     1561 tctcctaaag gaagagccag cttggttagg gaatggttaa ggcaattaag tgctttgaca
     1621 ggaaagcgag aattaacatg gccgttgaaa aagcgattaa atacttacaa aaggaatcac
     1681 ggccaatatg atttttccca tgaagtttat actgcgctag aaggatgtct gggatgccat
     1741 gcatgtcgtt ctcaatgtcc ggttaatatc aatgttcctt cgtttaaagc aaaatttttg
     1801 caaacctatc acacacgata ttctcgctca ataaaagatt atcttattgc ttacagtgaa
     1861 cgtgttggat attttcaaag cctgtttcca gaattagcta atttctgtct ctcaaataaa
     1921 ttagtaaaat attgtttaca taaatacaca gggttggttg atctcccttt gtttagtagt
     1981 ccaaatctga agtcactctt aaaggcaaat aatattccat ggcttgattt aaaccattta
     2041 cctgtcagtg agaaaactgt aatttatata caggattggg tgaccagttt ttatgaagct
     2101 gaacttgtgc tgaaagcttg tcagttaata aaaaaactgg ggttccaggt ttatgtatta
     2161 ccttggtttg aaaatggtaa aactctgcac gacaatggaa tgcttcagtc atttgcaaaa
     2221 attaccagaa aaaatgattt aatgcttcaa aagctatcag atcatcgatt aaccatgata
     2281 ggactagatc cagctatggt attaacctat cgatatgaat attctgaata tttacacgaa
     2341 aattacacta gtaaaatcct gttgattcaa gagtggctct cgcaaatact gggtagtgaa
     2401 aaagtcaacg agttaccaat aaaagctaat gaaaaactta atcagtattt attgctaagt
     2461 cattgcacag aacaggcaaa ttgcagtgaa gctttaatgc agtggcagaa agtctttcaa
     2521 ttttttggtt tgactctagt tgctgaaaaa acagggtgtt gtggtatggc tggagcttat
     2581 ggacatgagt caatccatca atttaattcc catggcttgt ttgatttatc atggaagaat
     2641 tattttgaag acagtactgg tttagctaat actttagtcg atggctattc atgtcgagca
     2701 caagtcaaac gattaactgg tttaaaagct tctcaccctg ttgaaatatt actgggatta
     2761 tttgagtagg tataagctgg aattgattat ctcagccaaa tatttaccga attactttct
     2821 catgcgggtt aatcctgcag catggtgcaa ctgttatcaa tttccagttg atgatgtcag
     2881 ttctgatttt agtcgttgat aatctgatgc aaacaggaat tggcctatat gaaatattcc
     2941 ataagtaata aatggggctg caaaaacatc aatactataa tgaatatgac caatcagcac
     3001 cgtataagcg aaaaaaactg cagcagcaag gcaaatataa cgtaatgttg tcttattcca
     3061 aaagataagc atcattaaaa aagggcctcc gacatgtcca gaaaaaaata aatctccatc
     3121 aaataaaaca agagatgaaa aattatctgg aatagacagt tgattaggag gtgcaccgag
     3181 atgggtcata ctaataaagg cacaacgtat caatataaac agcgcggcac ttttagtgat
     3241 aaaaggaatg aaggctggat ttaacagggt ataaatagta aaaattgtcc aaaagataat
     3301 agccgcatta acatgaaaaa gagttacatc acgcagaggt aaattatcaa gaattaaatc
     3361 atttaccgga ttaccggcca taagtgaagc atattttcct gcaaaattca taatgagaaa
     3421 acttataaaa agcaaaaaaa agccactaag cagacttctt atgaatactt tttcctgaca
     3481 aagaacatag tagcgatttg cgactgcttt cattaactca ccatcgattg atagagtgtt
     3541 tcatattgtt cgatagttcg tttgatatca tgctcttgag ataattttct gctctccctt
     3601 cccatttgtt cactgaaagt cctatcacta agaatcttga ccatataaca agacaatgtc
     3661 tcaacgtcat tagggtcaaa gagataacca tttactcctt ctttaacaag ttcaggtaaa
     3721 gccatcgctt ttgcagccaa aagaggcaat ccagaagcaa ttgcttccaa ggcaacaata
     3781 ctttgtaatt cagcagttcc cgggtttaca aaacaatttg ctaaactata taccagcgga
     3841 tattccgcgt ctgatagata tcctgtaaaa gtgacatgct cagtgagatt cagtgtctga
     3901 accagttttt ttaatctttg taattccgca ccacgaccgg ttaaaacaaa atgagcatca
     3961 atggattgtc ttgctttata aaatgctttt atggctattg ataaattttt ttctttatcc
     4021 aaacgaccag catacaatag aattggctta tcagggattt tataacgttg tctaatgaga
     4081 ttggcatttt gttttggttg aaatttttgt agatccactc cacaagaaac aacatgaatt
     4141 tctttttgta tgtgtacttc tttaagtaag ttagctgcag tgtgggtagg agtagtgact
     4201 atctttacat gatttagcat gtcaatcaca atattccatg tggtgcgatg aaaccatttt
     4261 tcaaaatact tgggaaggtg agtataatga aaaaaatttt ctggcataaa atgattagta
     4321 gccattaacg ggataccctt tttacgacag gctcgatagc aaattccacc taagaaaaat
     4381 tttccctgca aatgaacgac atcaggatta aagtctgtta ttgcttttaa aatacccttc
     4441 ttgatgaaga aaggccagca aacacgaaag tttttgtaac caagggctgg ccatgatcgt
     4501 actccataga gattgactcc ttcatagctt gtataacctt gcttcaggct tcgagagggg
     4561 catatcacaa gaacttcatg ccctttggct tgtaagaatt ttgccaaacg gtgagtaaaa
     4621 taagcaccac catttaagtc gggaggatag ctgtccagta cagtcaggat tttcatgcat
     4681 attttagata acatataaga ttagatagat tatcatttat acaaatgcac ggcaattatt
     4741 taccaaccaa cctttaaaaa gttctctttg actataatag gccttatagt acaataattg
     4801 ctccatcctt taacaagtca gttctatgtc gtcagcaaaa atcatcggaa tgggaaaata
     4861 tttacccgca aacatagtgt tttcatctga tctggataaa aaactcaatc ttcctgaagg
     4921 cagtgttcag aagaagtccg gattagtttc caggcatttt gccagagtca gtgaaacaac
     4981 ctcctatatg gcaagtcaag ctgcattaca cgctgtggaa caagcaggaa ttactttagc
     5041 tgaaattgat gccattattg gggcgtgtgg agccggagag caacctatac catgtacggc
     5101 ggttctggtt caaaaacaat tgggattaga taattcagga atcgcctgtt ttgatattaa
     5161 cagtacctgt cttagttttc tcactgcact cgatgcggca tcatatatgc tctcaggggg
     5221 cagatttaat cgtatactta ttgtatcaag tgatatccct tcatttggcc tggattggag
     5281 cgacttggaa acctgtacga tttttggtga cggggcagct gcttgtgttg ttgaaagaag
     5341 caatgactat agtcgaattc ttgctgctca tatgaaaaca tacagttttg gtgctgactt
     5401 ttgccaggtc caggctggag gaacattcat gccgcctgca agtgagtacg ataagaaata
     5461 cggtttattt aaaatggatg gaaagcgtgt attcaaactg gccagccaaa tgattggacc
     5521 catgcaagag gagttatttg ctaaagctag tataactcta aatgatgttg attgggttgt
     5581 cccacatcaa gccagcttgc ttgctatgca tcatatacgt aaaaagcttg gcatccctgg
     5641 tgaaaaattt gttgatattt acacgacgca tggcaatcaa atggctgctt ctctgccttc
     5701 cgcattgtgt catttgatcc ataatcataa tttagaacgc gggcagttgg tttatttatt
     5761 aggtactggc gctggattgt cagctgctgg aatgatactg gagtattagt atgatttcag
     5821 ttgtaaccgg cgctacagga tgtttaggtt taaatcttac aagacgcttg attaaggaag
     5881 gccatgcagt cattgcatta ggacgcaatt tgcagttggg tgaaataata agccaaatag
     5941 gagcaacatt tgttccactc gatttaaagg atctggagtc tttgtctttg ataagtcaaa
     6001 atgcggattt catatttcat tgcgcagctt tatcaagccc ctggggaaaa tataaagatt
     6061 tttatcaggc taatgtcata gggactcaca atgtcgttca ggcaactccc agtcaggctc
     6121 gacttgttta tgtctccact cctagtattt attttgattt taccgagaag cacaatatta
     6181 aagaaaatgc acttttacca gcaaaaccgg tcaattatta tgtgcaaacc aaattaattg
     6241 ctgaatccat cgtagataaa gctcaactac agcatgatct ggatgtgatt accatacgtc
     6301 ccagaggaat ttttggacca tatgatcgcg ctattttccc ccgtttattg aaagcagagc
     6361 gccaaggggt gcttccaatc attggttcag gtaatcattt gattgatatt acttttgtag
     6421 agaatgtagt ggaaagcctg attttggctg ctttggcaga taaatgctat tcagggaaaa
     6481 aatataatat cactaatgat gaacctcgga cttttattga tatcatttca aggatgtttt
     6541 ctgctcttaa taaaccatta aaaaccagaa gcattcctta taatcacgca agatttgtcg
     6601 caaagtttct tgagtttttg catcggttgc tttatctgaa aacagaacca aagattacag
     6661 agtatggtgt cggagtgttg gcttttggac aaacattaaa tattgaagag gcaaaaaaag
     6721 atttaaagta taagccaatt tatagcattg atgagggtat aatgaaattt gctgaatggt
     6781 attgttcatg catatagagt atcagttatt tgaagctggc tattgcaaac attgcgaacg
     6841 catgacttta aaaaatggga aaatcagaca gcgtgaatat ccatccttct gtgctcttat
     6901 aaaacaccca agtcacggat atattttatt tgacacagga tattctgacc gttttacctg
     6961 cttaacccaa aaatttccag cctctatata tcgtcattta actcctatga cacttgtaaa
     7021 atccttaaaa gaacagttgc tagaaattca tataaaaccc gaagatatca attatattgt
     7081 gatttcgcat tttcatgctg atcacattgg aggattatgc gattttccta atgctcattt
     7141 tatatgtcat tctgatgcga tagacgatat tcagcataca aaggggttga aagcgttatt
     7201 gaaaggtttt ttacctggac tgatacctga agatttcttt gagcgtttat taccacttca
     7261 aaaaacaatc caacttgacg agcatctttt acctttttct gatggttttg atgtattggg
     7321 tgacaactca ttgattgcta ttcctttacc gggacacgct aaaggacaaa tcggcttata
     7381 ttttaaagcg cctggcgaca ttttcctggt tgcggacagt tgctggcacc aggaaacttt
     7441 taaagaactc atttatccga gcgctttgac ctatttgatt catcatgaca agcgtgccta
     7501 tttaaagaca attcaaaaat tgcacgaact acatcagaga aacaagacta ttgaaattat
     7561 tccttctcac tgccaacaat tgcgctcgag aattgggaac ccattatgtt agcacgtatt
     7621 ctatttcact atttcaatac actttatctt aataaaacta ttactacccc ggcaaaatta
     7681 aagcttcatc aagcaaaaca atttaaaaaa cttataaaaa attgtttgca tcaatccgca
     7741 ttctatagag attatctaaa aactcctttg gaacaatggc caattgttaa taaaaaaatc
     7801 atgatggaac actttgatga tgttaataca gtaaatgtca aaaaaactga ggcattggaa
     7861 attgctttac gtgctgagca tacacgtgat ttttccccaa tgataaataa tattgctgtt
     7921 ggtttatctt cagggacttc agggagcaga ggcttgtttt tagtaagtga acaggaacgt
     7981 gatgcctggg ccggaataat ccttgccaag gctatgccaa acggtttgcg ttcgcgagaa
     8041 cgaattgctt tttttctaag agcaaataac cagttgtata ccactttaaa taaaagcaga
     8101 acaatacaat tccatttttt tgatcttttt gctgattttg atactcatat ccagagatta
     8161 aatgccattc aaccgactat tatttcagct ccagccagtg tgcttttaat gcttgctagt
     8221 caacaagata ttttaaaaat tacaccaaaa aaaatatttt ctgtagccga agtactcgaa
     8281 ccaaatgaag aagagatttt aaccagagca tttggttgta ccatatcaca aatttatcaa
     8341 tgtacagaag gttttctggc tattagtgaa cgtaagacca atcgcttatt gatgaatgaa
     8401 gagtttctta ttattgaaaa ggaatggttg gatgaagagc gattcgtgcc aattattact
     8461 gacttgtaca ggtctactca accaattatt cgttaccgtc ttgatgacgt tttggtgact
     8521 gaaagatcga atggtgtatt tactgagtta aagaatattg aaggtcgttt aggtgatgtt
     8581 tgttatggga aaagagggca agaaatagtt cccatttttg cagatgttat tcgacagcat
     8641 atgattagca gtggtgtaag ttttgaagat tatttaattt gccagcagac attaacccaa
     8701 tttacaattc agatatcacc cgaaataagc aataaaaatc aattcattga acatttgaat
     8761 caacttttta tctataaaaa ttgcgaatta ccccaatgga actgggagct ctacgataaa
     8821 agtcaacctg gtgtgaaacg cagaagggtc cgctcactat actgccctaa aacagaataa
     8881 gcgggtacaa agaactggtt tgctttgcac aacacaagcc aggagcttac aataatattt
     8941 gcgtctgtaa aagacaggct cttaatttag tcgttaagcc ttttttactg tgaatgcaaa
     9001 tggcgcgtag agtactttcc catcaatttg cacctgggca aacattttaa tgaaacctgc
     9061 tttattaggc tcaatatgga actgtaattg aggtcctcca cgttcagtgc tatttgcagg
     9121 ctctttgccc atggggtgga catggataac ggttttaaaa tcttcattaa agccgacaat
     9181 gtgtgcatag gcccccataa ttggttctag tgtacggact ggatgccctt tagaatcagt
     9241 cacatcgatt tcacccatcg caggtttacc tgctagtaga ggcattttat tgaaagttaa
     9301 tttgaattga tatccattca ctgtactctc taatactgtt cgatgatgta taggattcat
     9361 tgttcttgtt actgtctggg gagatgttag atcagcagtg gcatactcct gtttattggt
     9421 attggttggt aacagatcag cccatgcccg ataggtggca ttcttgaaat tgggttgcca
     9481 ttcaaattga taaatccccg gagtagcagt ttctgcggga tgaacatgac tatagtcaat
     9541 aagatgatta tcaatgatca gtaaatgaaa tttttttgta tgtatctcag taagatcatt
     9601 gaaagtaatt ggtttattcg attttatatc agtgagtttg acaaatacca gtttcttatc
     9661 ctttttatca attatcttct ggacttccaa ttgtattgtg ggtgtattct ccaatttaga
     9721 ggaaggcgtt gaaagatctt tcaccgtgtg atgatgggaa ttatcggcat agagcaaatg
     9781 atgacaggtg ttcatgaata aaaagccaat tagcgtgatg agaatttttt tcacaataca
     9841 atgatctccg taatttttta ttgccaagct aaatttttct tctgtcaacg atgaattata
     9901 actgaaagta gctgattctt acagggtaag aaatgcatta tttattatga ccttgtagat
     9961 ataaaccagt ataacagggt atcatgagct aatttaaatg gttataaatt ccaggcagaa
    10021 taaaactacg gataggtgaa taatggcaca caaccaccat gaaggtcatt cccatgttca
    10081 taatgaacac ctttgttgcc acaagaaaga taatgcacat aaaccgcaaa ttatttcaga
    10141 tggaacaatt ttctatactt gccccatgca tcccgaagtt cgccaggcta ccccggggat
    10201 ttgtccactt tgtggaatgg cattagaacc agaagcaatt tccattggtg aagcaacaaa
    10261 tccggaatac cttgatatga agcaccgatt ttgggtggcg ttgatattga ctttgcctgt
    10321 agtaatcttg gaaatggctg ggcatggatt ccagcatttt gtttcgacag gtatttcaaa
    10381 ttggattcaa ttgttgttcg caacacccgt ggtgctatgg tgcggttggc cttttttcct
    10441 gcgtggttgg cagtcactca aaacacgccg attaaatatg tttactttga tagcgatggg
    10501 tattggtgtc gcatggattt atagtgtggt ggcggttttt ttccccggat tattccccgc
    10561 tgcgtttcga agtcaggaag gggtggtctc tgtgtatttt gaagcagccg ctgtgatagc
    10621 cacacttgta ctgctggggc aagtacttga attaaaggct cgagagcaaa caggtagtgc
    10681 cattcgtgct ttactgaaat tagcaccaga aaatgcgcat cgcatcaagg aagacggtag
    10741 tgaagaggaa atagcactca atcaagtaga ggtcggtgat tcgctgcgtg tacggccagg
    10801 tgaaaaaata cctgtggacg gtgaaataca agaaggacgt agttttatag atgaatctat
    10861 ggtaacgggt gaacctatgc ctgttgctaa aggaattgga gacaaggtta ttggcgctac
    10921 aattaaccaa acaggtagct tcattatgaa agcattgcat gttggtgcag ataccatgct
    10981 ggcacgtatt gttcaaatgg tgagtgacgc tcaacgaagc cgtgctccaa ttcagcgtgt
    11041 ggcggatact gtctcggcct ggtttgttcc cgcagtgatt ttggtagcgg tactgtcttt
    11101 tattacatgg gggctattag gtccccagcc atctcttggt tatggcttaa ttgctgctgt
    11161 atcagttctc attattgctt gcccatgtgc gttgggtttg gcaacaccaa tgtctatcat
    11221 ggtaggtgtg ggaaaaggcg cgcaaaatgg agtattaatc aaaaacgctg aagcacttga
    11281 acgaatggaa aaggtaaata cgctcatcgt agataaaacg ggcacactta cggaagggca
    11341 cccgaaactt acccatattg aaaccagcga agattgggat gagagacaag cattgctatt
    11401 agctgcttct ctggaatatc acagtgaaca tcccctggct agcgcgattg tgaaagcagc
    11461 acaagaaatg caaatagctt ttatctcagt tcaaaatttt gaagcgccaa ccgggagggg
    11521 ggtagtaggt agagtaaata gccatcatgt agctatcggt aatgcaaaat taatgcatga
    11581 gtatggaagc gacgatactt ctctttttga aaaagctgat aaatttcggg caaaaggttc
    11641 ttctgttatg tttatggcag tcgacggtaa aacagtagcg tttttagttg tagaagatcc
    11701 tattaaatct actaccgcag aaaccattca tgcacttcag aaaaagggta ttgagattta
    11761 catgctgact ggtgatagca agaaaaccgc agaagctgtg gcagctactc tgggcataaa
    11821 aaatgtcata gcagaaataa tgcctgaaga taagggtcgt attgtcagcg aattaaaagg
    11881 aaatggttta attgtcgcga tggctggcga tggagtaaat gatgctcctg cactggcaaa
    11941 agcagacgtt ggtattgcta tgggtacagg gactgatgtt gctattgaga gtgctggtgt
    12001 taccttgcta catggagatt tgagtggtat tgccaaggcc agatatttat cagaaagcac
    12061 aatgaataac atacgtcaaa atctcttctt tgcttttatt tataatatgc tgggtgttcc
    12121 gctagcagca ggtattttct acccactaac aggcctgctt ttaaatccag tgattgctgc
    12181 gggagctatg gcactttctt ccgtgtcagt catcgttaat gccctaaggt tgcgacgagt
    12241 tacattgtaa atctgtaaat aaaatatttt ggtaaagtgc tttaatctcg tgcaatgatt
    12301 cctggttata ctaattgaa
//
LOCUS       DACTJE010000094        12142 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.094, whole genome shotgun sequence.
ACCESSION   DACTJE010000094 DACTJE010000000
VERSION     DACTJE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.094"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            61..1083
                     /locus_tag="I9760_001933"
     CDS             61..1083
                     /locus_tag="I9760_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAT5043697.1"
                     /translation="MHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVWLNP
                     SAEAMLGYGLIGSLWVDVIQKAFVPRADDGHEVSLADGRRVHVALSSLESLPGILITL
                     SDITASRDYEEAKENEKRLVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDHRIK
                     NWIHRLQECHASIEQQIQDLLLFARGTSIEPKYIDMEFWCSQLMQRAQPYIESYAAVL
                     HVNNHLIARESSIHGESLIGAILNLIINALQSEATQIDLTLASMDDSGIQISVEDNGN
                     GMSEEVRAQAFSPFYTTKAQGTGLGLAVVFAVVKAHGGRVTLESTEGIGTQVTIYLPC
                     LNRNVG"
     gene            1107..2420
                     /locus_tag="I9760_001934"
     CDS             1107..2420
                     /locus_tag="I9760_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAT5043698.1"
                     /translation="MSHVLIVEDDSVLREALAETMTISGHTYITAKDGKEALALLELH
                     NPSVVLTDIRMDKLDGNQLLNEIQKKRPGLPVILMTAYGSVEDAVTAIRNGAVDYLQK
                     PFSAQVLIEKIKQYIKTDLSEEDNDPIARDPKSQALLTMALRVAQSDVGVMISGESGT
                     GKEVLAHFIHDHSYRSKQPFIAINCAAIPEQMLEATLFGYEKGAFTGAYKSTPGKFEQ
                     AQGGTLLLDEVSEMSLGLQAKLLRVLQEKEVERIGSNKIINLDVRIIATTNRQLKEEV
                     QAGRFREDLYYRLNVFPLQWHPLRERTSDIIPLANYLIRKHCQHKQPIVPVISPDAQK
                     AMLSYSWPGNARELDNVIQRALVLQTQGIIEEEHLQITNSTTMPSEEKTKGTSKNLQI
                     HEYELIEKTLLENEGNRQKVAALLGVSERTLRYKLAKMREEGYVV"
     gene            2435..2749
                     /gene="fliE"
                     /locus_tag="I9760_001935"
     CDS             2435..2749
                     /gene="fliE"
                     /locus_tag="I9760_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAT5043699.1"
                     /translation="MSDINTVNLINQMKTLAAQAEGIKPDSGLNHASFSSVFQQALNQ
                     VNELSQTADSLKTRFEIGDPNVSLGEVMIASQKSNLGFEATLRVRNKLVQAYQDIMNM
                     PV"
     gene            2921..4555
                     /gene="fliF"
                     /locus_tag="I9760_001936"
     CDS             2921..4555
                     /gene="fliF"
                     /locus_tag="I9760_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HAT5043700.1"
                     /translation="MALADNASVAATKFASLSIPRQIGVLFGLAASIAIGVAVVLWSR
                     DPSYVPLYSQLNAQDSSEILTILERNSIDFKIDKNNGLLMVPADELQSARLKLAAQGL
                     PRDTAIGHDLLGSSGGVFNTSQFMENARYRQALEAELARTISKFNNIKSARVHLAIPR
                     ESAFVRDSQEPSASVFIDVYSGFEIKKHTIAAIVNLVASSIPNLSAGRVTVVDQDGQL
                     LNEGSGYNLFSETERFLDYRQNLEHQYAQKIQDILTPILGYGRVRAKVSADIDFTSYE
                     QTQELFNPELSALRSEQTMQESRNASNEASGVPGTLSNTPQPNSSLGQKNNPQQNATP
                     NNLPQQTSSDQMQAKDVRMQTTKNFELDKTISHTKNQPGTIKRLSVAVLVDNRPVMND
                     KTKKLEKKPLTKEEIEQIKLLVSDAIGLNVKRGDSLNVINSSFVKPDPIEAIPEEHFW
                     QKDSFWSVIKQIGGALFILAILFGVLRPMLKTLASNKDMDQATDNQDTGDFTLDGKIS
                     FRDVQDYETQLSLLRQVVDKEPKRVAQVVKSWVDRG"
     gene            4558..5547
                     /gene="fliG"
                     /locus_tag="I9760_001937"
     CDS             4558..5547
                     /gene="fliG"
                     /locus_tag="I9760_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAT5043701.1"
                     /translation="MDGIERAAILLLSMGEKNASEVLKHLEPRQVQKVGMAMSTMNSV
                     SKAKMQNVLIDFISAIEDQTSLTVDTEHYLRTVLIEALGEEKAIPFIDRILVSNKDSG
                     LNKLKWLDGRLIAEVIRNEHPQIIATILIHLDSEQSAEVVSYFSAEKRAEVLLRMCNI
                     DTVKPEAISELGHVIEKQLSGQTTGKSSSVGGIKTVADVINFLDGAMEEEVLERIKDW
                     DEELSEKIRDKMFVFENIKDMDDRSVQTLLREVTSDQLKLALKGTTETTKEKIYSNMS
                     KRAADLLRDDIEVQGPVRVSDVERAQRDILAIARNLAEDGKISLGAKGGDELI"
     gene            5689..6327
                     /locus_tag="I9760_001938"
     CDS             5689..6327
                     /locus_tag="I9760_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAT5043702.1"
                     /translation="MSNEFEPYNKEIKKIEFTTWKYQASKDAEHSPSDEEELINELEV
                     VKQEAFQKGYADGFQQAQEEINAIKAELSKWLDLIQKPVQILDEQLTQEIIQTIIWLS
                     QHCVAVELSVNPDKLNDLLNKIKGELPSLTGKKSISMHPDDVRWIKEVFSESNIPGLH
                     ELLVADPALNRGDFYLQGEHSELDGRIQTRFTTLFNEYINKENIIPMGVSQE"
     gene            6330..7679
                     /gene="fliI"
                     /locus_tag="I9760_001939"
     CDS             6330..7679
                     /gene="fliI"
                     /locus_tag="I9760_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HAT5043703.1"
                     /translation="MAIYQTQLHKILSGTRNKHHSGLLHCGRISRAIGLTIEAKGFNQ
                     PVGARCLVKINDEHSVQAEVVGFSNESVYLMSIGDIQGIAPGMKIIPTGRIAQVSVGH
                     QLLGRILNGSGMFIDDGPPGEMVETYPLIGQVINPLKRAPIEVPLDVGIRAINGLLTV
                     GRGQRIGLFAGSGVGKSVLLGMMTRFTEADVVVVGLIGERGREVKEFIECNLGLEGLR
                     RAVVVAAPADESPLMRLHGAKVATSIAEYFRDQGKHVLLLIDSLTRFAQAQREIALSI
                     GEAPATKGYPPSVFAKLPKLVERAGNGTSGGGSITAFYTVLTEGDDLQDPIADAARAI
                     LDGHVVLSRALAEQGQYPAIDLEASISRVMQGVVSEQHMKDMLLFKKYLSVYEKNKDL
                     ILLGAYAKGSDPLVDKAILAVNKLKDYLAQGMDEQVSYDESLSSLTKLISTLVGNDQ"
     gene            7676..8131
                     /gene="fliJ"
                     /locus_tag="I9760_001940"
     CDS             7676..8131
                     /gene="fliJ"
                     /locus_tag="I9760_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HAT5043704.1"
                     /translation="MSDRLDRLIQLLKIKQEATQQAYIELVKAREQFNQNKARHEQLV
                     GYRQDYLQQLEVLGQQGSYVGPLRNRINFINHLDTALVQLNSYLSQLAKNRMKADLNY
                     KQAKTSEEGISKLIERVKRAELKQLQRIEQKETDEYAQKQWYSSLKHDR"
     gene            8121..11942
                     /locus_tag="I9760_001941"
     CDS             8121..11942
                     /locus_tag="I9760_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02099 family protein"
                     /protein_id="HAT5043705.1"
                     /translation="MTDNNLLANKFFKKIWMVLAVLIIVAAVFSSVFRALTPWAKQYK
                     GEVEQHLTSLLGQPVTVQTMETGWYWFEPVVKLKHIRIHDGHNKSIYLDRLIVGVNLF
                     KSLWNWRIQPGVLYIDDMHLVFREKDGHWCIEGISRNAMKDTDITPEKTREILDWLAQ
                     QEKLIVRHVSAHLHFNDGSLIPVNELNLSIVNKGGNYKIKGEARLEQTNATVFQLLGN
                     VQFDPSQIKNTNGRFYFSAINVLPAQWQNLLPKSSMQLEGGKGNVSLWVDLEKGNISS
                     IQAELRLKRLAWKLLNKKNSKLIQSFYANMGWKLDKQGWQFQADHMKLRVAGVTWPEN
                     QLSIRFNKNQNTYQIFTKSLIVESLLSEAINWPPAIQNVLQLKPHGILSDVQVLIKEN
                     QINYILTRFDQLGWRAKDNIPGVSNLSGVLNWQPTEGRLELDSENSTFDIKGYPAQKL
                     TIFNGAFDWKELNDGLRLSIERLVASQPELTISLEGAIDQLTQNSVGNIRLVGNFSGK
                     NLQKWMPYLPKKHMKAKLFAWLNNDIKRLGQATGKITINGMAKDFPFDNGNGEFSIVS
                     HVVGGDILITPEWRLIKDLEGYIKLKNRNLDIDIIDGDAQGTLLKQMNLRIDDIGKDR
                     ETLLIHGIVHGKAQQMLNFVMNSPLKKKLSALKILLIKGMLSLNLRLEIPLYPENDDN
                     LARGELTFENNIINIKHQMGDFSVRDVTGNLLFNEKGIQDSALTARAFGYPLNIKIQS
                     EKSPQPLTAISIEGECTVESLKKKWHLPIFSVLKGNILAKALLKITDDPNDLDNMKLS
                     SDLIGLAVDLPAPLGKTYKEKTPLQVNLDFNPQKTLRLRVNYNGRLSTDLLMQKQKGV
                     FKLKSGQIRLGSAQAVNQDHPGLGIAGTLDGFDLQAWKEVINRFSKENVNPSLLSLVN
                     IIDVKMGKLNFLNQQFDNMSLYAKKRPDNDWSINLKQKKVDADLTYHSATNSLSGFVK
                     YLHLSEIGSSKNNKDTPSHTTPGQIPNLNLRVDNFSIGKIQIGNITLKSHSTSEQWKI
                     DYCRIDSPYYQANFEGEWLQKGKFNQTKLQSKMYLKDLAKSLDRWAINPAVDAGKGYI
                     EFHGGWQGSLYDFSLSKLNGSMYLQLKNGRITHLSPETEEKLGLGKLLSILSLQTIPR
                     RLKLDFSDLSNDGYSFDIFKGNFNVNNGIMSTNNSYIDGPVAYASMKGDLDLVRRLYD
                     LDLNISPHITASLPVVATIAGGPIAGLAAWIANKIINQSMQKIAGYSYKISGPWDQPI
                     VQQLNIVKKLP"
BASE COUNT         4055 a         1953 c         2667 g         3467 t
ORIGIN      
        1 ttttaatttt ggcctgaact ttgctagaga catttcataa taataattaa aatgaataac
       61 atgcattatt caaattatca aacttgtgca gtcaaaccac agatagaatt atctgaatgg
      121 gacattttgt ccagtttgcc taccgccttg gtaattttga atgcccaagg caaagtggtg
      181 tggttaaatc cttcagctga agcgatgctt ggttatggac tgattggttc actatgggta
      241 gatgtgattc aaaaagcatt tgttccaaga gctgatgatg ggcatgaagt gtctctggct
      301 gatggaaggc gtgtgcacgt agccctttcg tctttagaga gtttacccgg tatattgatt
      361 actttgtctg acattactgc aagcagggat tatgaagaag ccaaagaaaa tgaaaaacga
      421 ttggtttcta ttggtacaat gaccgcacaa ttagctcatc agattagaac tcctttgtct
      481 tctgccatat tgtataccga gcatttaaat aatctcccaa gtttggatca tcgcattaaa
      541 aactggattc acagattgca agaatgtcat gccagtatag agcaacaaat tcaggattta
      601 ttattatttg ccagaggaac ctccatagag ccaaagtata tcgatatgga gttttggtgc
      661 tctcaattaa tgcaaagagc tcagccatac atcgagtctt atgcagctgt attacatgtt
      721 aataaccatc ttattgctcg tgaatcctcc atacatggag agtctttgat tggagcaatt
      781 ttaaatctga taatcaatgc gctgcaatct gaggcaaccc aaattgattt aacacttgca
      841 tccatggatg attcaggtat acaaatatca gtagaggata atggaaacgg aatgtctgaa
      901 gaagtcaggg cacaagcatt ttcgccgttc tatacaacaa aagcacaagg taccggcctt
      961 ggattagcag tggtttttgc tgtagtaaaa gcgcatggtg gtcgagtgac tcttgagtcg
     1021 actgaaggga tagggacaca ggtaactatt tatttacctt gcctgaacag aaacgtaggg
     1081 taactttatc aaacaaagga ttttctatga gtcatgtttt aatagttgaa gatgattcag
     1141 tattaagaga agcgttggct gagactatga cgatctcagg ccatacctat atcactgcta
     1201 aagacggtaa agaggcatta gcacttcttg aattacataa tccaagtgtc gtactcactg
     1261 acataagaat ggataaatta gatggaaatc agttattaaa tgaaatacaa aaaaagaggc
     1321 ctggtttgcc ggttattcta atgacagctt atggttctgt agaggatgcg gttacagcga
     1381 ttcgcaatgg cgctgttgat tatttgcaaa agcccttcag cgcgcaagtc ttaattgaaa
     1441 aaataaagca gtatataaaa actgatctga gtgaagagga taatgatcct attgcacggg
     1501 atcctaaaag ccaagcctta ctgacaatgg ctttgagagt cgcacaatcc gatgtgggag
     1561 taatgatcag tggagaaagc ggtactggaa aggaagtgct cgcacatttt atccatgatc
     1621 attcttatcg aagcaaacaa ccttttattg caattaattg tgctgcaatt cctgaacaaa
     1681 tgctggaagc aaccctgttt ggttatgaaa aaggtgcttt taccggagca tacaaatcaa
     1741 cccctggcaa gtttgagcaa gcccagggcg gaactttact tttagatgaa gtaagtgaaa
     1801 tgtccttagg tttgcaggca aaattactta gagttttaca ggaaaaagaa gtagaaagga
     1861 ttggttcaaa caaaataata aaccttgatg tgcgcattat tgcgacgaca aacaggcaat
     1921 taaaggaaga ggttcaggca gggcgtttta gagaggacct ttactatcga ttgaatgttt
     1981 ttcctctgca atggcaccca ctaagagaga gaacaagtga tattattcca ttagcgaatt
     2041 atttaatccg taaacattgt cagcataaac aaccaatagt tccagtcatt agcccggatg
     2101 cgcaaaaggc aatgttgtcc tattcatggc cagggaatgc gagagagctg gataatgtta
     2161 tacaaagagc tctggtgtta cagactcaag gtatcatcga agaagagcat ttgcagatta
     2221 cgaattcaac taccatgcct tcggaagaga agacaaaggg tacgagtaaa aatttacaga
     2281 tacatgagta tgaattaata gaaaaaacat tattagaaaa tgaaggaaac agacaaaaag
     2341 ttgctgcgtt attgggggtt agcgaaagga ctttgcgcta taaactcgca aaaatgcgtg
     2401 aagaagggta tgtggtttaa caggggctga gagaatgagc gatattaata cagtaaattt
     2461 aattaatcaa atgaaaactc tggccgccca ggctgagggg ataaaaccgg attctggttt
     2521 aaatcacgct tctttcagtt cggtttttca acaggcatta aatcaagtca atgaattatc
     2581 acaaacagca gatagtttaa agacccgctt tgaaataggc gatcctaatg tgtccttggg
     2641 tgaagtaatg atagcaagtc aaaaatccaa tttagggttt gaggcgacat taagagttag
     2701 aaacaaattg gtacaagcat accaggatat tatgaacatg ccggtttagt gcgatgattt
     2761 gggtagtaga actttttcaa cagataaaca agatcaaact caataacagc tttttatggg
     2821 gtttttgaat gccataatga gtttgcaaat gttctcaata aagttttaaa gctcagagtg
     2881 aagtattctg aaaaaaggat aaaaattaca ggagtaaatc atggcattgg ctgataatgc
     2941 ttcagtagca gctacaaaat ttgcaagctt atctatacca cgccaaattg gagtgttgtt
     3001 tggattggcg gccagtattg caataggcgt agcagtggtt ctatggtctc gtgatccaag
     3061 ctatgttcct ttatatagtc agctcaacgc acaggactca tcagagattt tgactatttt
     3121 agagcgcaat agtattgatt tcaaaattga caagaataat ggcttgctga tggtgcctgc
     3181 tgatgagctg caaagcgcgc gtttgaaatt ggccgcacaa ggattgcctc gcgatactgc
     3241 cataggtcat gaccttttag gcagcagtgg tggggtgttt aataccagcc aatttatgga
     3301 aaacgcccga tataggcaag ctcttgaggc ggaattagca cgcacgatca gtaaatttaa
     3361 taatattaaa tcggccagag tacacttggc aattccaaga gagtcagcct ttgtaagaga
     3421 ctcacaagaa cctagtgctt ctgtatttat tgatgtgtat tcaggatttg aaataaagaa
     3481 acataccatt gcagcaattg tcaatttagt agcatcaagt attccgaatt taagcgcagg
     3541 aagagtaaca gtagttgatc aagacggaca attgctaaat gagggtagcg gatataacct
     3601 cttttcggag actgagcgtt ttcttgatta cagacaaaac ctggaacatc aatacgctca
     3661 aaaaattcag gatatcttaa ccccaatatt gggatacggc cgtgtcaggg ccaaagtatc
     3721 tgcagatatt gactttacca gctatgaaca aactcaagaa ttgttcaatc cggagctctc
     3781 cgctttacgc agcgaacaaa ctatgcaaga gagtcgtaat gcgtcaaatg aagcaagcgg
     3841 ggttcctggg acattatcaa atactcctca accaaatagt tctcttgggc aaaaaaataa
     3901 tcctcaacaa aatgcaacgc caaataactt acctcaacaa acatcgtcag atcaaatgca
     3961 ggcaaaggat gttcgcatgc aaaccactaa aaatttcgaa ttggacaaaa caataagtca
     4021 tactaaaaat cagccaggaa caataaaacg cttgtcggtt gcggtattgg tagataatcg
     4081 acctgtaatg aatgataaaa ccaagaagct ggagaagaaa ccgctaacta aagaagaaat
     4141 tgaacaaata aagttattgg tttctgatgc cataggattg aatgttaaga ggggagatag
     4201 cttgaatgtg atcaacagca gttttgtaaa gccagatcct atagaagcaa ttccagaaga
     4261 acatttttgg caaaaagaca gtttttggtc tgtgattaag cagattggcg gggctctttt
     4321 tatattggca atcctatttg gtgtattaag acctatgtta aaaacattgg caagtaataa
     4381 agacatggat caagccactg ataatcagga tacaggagac tttacattag atggaaaaat
     4441 atcattcaga gatgttcagg attatgaaac tcagctgagt ttgcttcgtc aagtggttga
     4501 taaagagccc aaacgcgtcg cacaagtagt gaaatcatgg gttgatcggg ggtaattatg
     4561 gacggaatag agcgagcagc tattttattg ttaagcatgg gagaaaaaaa tgcttcagaa
     4621 gtattaaagc atttagagcc caggcaagta caaaaagttg gaatggctat gtcaacgatg
     4681 aatagcgtca gcaaggcgaa aatgcagaat gttctgattg actttatcag cgcaatcgaa
     4741 gatcaaacca gcttaaccgt tgatacagag cattacctca gaaccgttct cattgaggca
     4801 ttgggtgaag aaaaggcaat tccatttatt gatagaatat tggtatcaaa taaagacagt
     4861 ggtttgaata agctgaaatg gttggatgga cgtctgatag ctgaagtgat tcgtaatgaa
     4921 catccccaaa tcattgccac tatattaatt catctggaca gtgagcagtc cgctgaagtg
     4981 gttagttact ttagcgcaga aaaaagagca gaagtgttat tacgaatgtg taatattgat
     5041 acagtaaaac ctgaagcaat ttctgaactt ggacatgtga ttgaaaagca gcttagtggc
     5101 caaaccacag gtaaatcatc gtctgtcggc ggaattaaaa ccgtagcaga tgtgattaat
     5161 tttttggatg gcgctatgga agaagaggta ctggagagaa ttaaggattg ggatgaggag
     5221 ttaagcgaaa agataagaga taagatgttt gtttttgaaa acatcaaaga catggatgat
     5281 agaagtgtac aaactttatt gcgagaggtg acatctgatc aattaaaatt agcattgaag
     5341 ggtactactg aaacaacgaa agaaaaaatc tattcgaata tgtctaagcg agcagctgat
     5401 ctcctcagag atgatatcga ggtgcaagga cctgtaagag tcagtgatgt ggagcgtgcc
     5461 cagcgagata ttttagccat agcaagaaat ctggctgaag atggcaagat tagcctggga
     5521 gcaaagggcg gagacgaatt aatctagtta cttttcccag gatgagtttt agacatttta
     5581 tgaaaatagt aaaaatcatt aagcaagagt ccgtatcaaa attgtatttt atttgtccta
     5641 agtggtaatt tttggtatga tttgggcccg ggataaggtt agataactat gtcaaatgaa
     5701 tttgagccgt ataataaaga gataaaaaaa attgaattta ccacttggaa gtatcaggct
     5761 tccaaagatg ctgaacattc gccgtctgat gaagaagagt tgattaatga attggaggta
     5821 gtgaagcagg aagcttttca aaaaggttat gcagatggat ttcaacaagc acaagaagaa
     5881 attaatgcaa ttaaagcgga gctctccaaa tggctggatt taattcaaaa gcccgttcaa
     5941 attttagatg agcaattgac gcaggaaatc attcaaacca tcatttggtt aagtcaacac
     6001 tgtgttgctg ttgagctttc tgttaatcca gataagctaa atgatttatt aaataaaata
     6061 aaaggtgagt taccttcttt aactggcaag aagtctataa gcatgcatcc tgatgatgtg
     6121 cgatggatta aagaagtatt ttctgaaagt aatatccccg gtcttcatga gttattggtg
     6181 gctgatccag cattaaatcg aggagatttt tatttacaag gtgaacacag cgagttagat
     6241 ggtcgaattc aaactcgttt tactacctta ttcaatgaat atattaataa agagaatata
     6301 atcccaatgg gcgtttctca ggaatagtga tggccattta ccaaacacaa ctccataaaa
     6361 ttttgtcagg aacaaggaat aaacatcatt ccggactgtt acattgtggt agaatcagca
     6421 gagcaatagg tttaaccata gaagcaaagg gatttaatca gcctgtagga gcgcgttgcc
     6481 tggttaaaat caatgatgaa cattctgttc aggcagaagt tgttggtttt agtaatgaaa
     6541 gtgtttattt aatgtctatt ggtgatatac aaggtatagc ccctggaatg aaaattattc
     6601 ctaccggacg cattgctcaa gtaagtgttg gccatcaatt gttaggaaga atattaaatg
     6661 gttcgggaat gtttatagac gatggacctc ctggtgaaat ggttgagact tatccattga
     6721 tagggcaagt gatcaatcct ttaaaaagag cccctattga agttccattg gatgttggga
     6781 ttcgtgctat taatggctta ttaactgttg gccgcggaca acggatagga ttgttcgctg
     6841 gaagtggagt tggcaaatca gttttgcttg ggatgatgac gcgttttact gaagctgatg
     6901 ttgttgtggt tggattaatt ggagaacgtg ggcgcgaagt aaaagaattt attgagtgca
     6961 atttgggtct ggaaggtttg aggcgagcgg ttgtagttgc cgctccagcg gatgaaagcc
     7021 ctttgatgcg tttgcacggc gcgaaggttg caacaagcat tgctgagtat tttcgggatc
     7081 aaggtaaaca tgttctcttg ctgattgatt ctttaacccg ttttgcccag gcacaaagag
     7141 aaatcgcgtt atcgattggt gaggctcctg ctacaaaagg atatccgcca tcggtatttg
     7201 ccaaattgcc taaattggtt gaaagagctg gaaatgggac ttcggggggt ggttcaatta
     7261 cggcatttta tactgtcttg actgagggag atgatttgca agatcccata gcagatgcgg
     7321 cacgagctat tttggatggt catgttgtat taagcagagc gcttgcagag caagggcagt
     7381 atcctgcaat agatcttgaa gcttctatta gccgagttat gcagggtgtt gtctctgaac
     7441 aacatatgaa agatatgtta ttgtttaaaa aatacctgtc tgtttatgaa aaaaacaagg
     7501 atttaatttt attaggagcc tatgcaaaag gaagcgatcc cctggtggat aaagcgatat
     7561 tagcagtgaa taaactcaaa gattacctgg cgcaaggaat ggatgaacaa gtaagctacg
     7621 atgagagttt atcatcgcta accaaactga tttcaacttt ggtagggaat gaccaatgag
     7681 tgacagatta gatcgtttga ttcagttatt aaaaattaaa caggaagcga ctcaacaggc
     7741 ctatatagag ttggtgaaag caagagagca atttaatcaa aataaagcaa ggcatgaaca
     7801 attagttggt tacagacaag attatttaca gcaactggaa gtattggggc aacaaggaag
     7861 ttacgtaggg cctttacgca accgaattaa ttttattaat catttggata ctgctttggt
     7921 acaattaaac tcctacttga gtcaacttgc aaaaaacagg atgaaggcgg atttaaatta
     7981 taagcaagca aaaacttcag aagaaggaat aagtaagtta attgaacgcg taaaaagagc
     8041 tgaactgaag caattacagc gtattgagca aaaggaaact gatgaatatg cacaaaagca
     8101 atggtatagt agtttaaaac atgaccgata ataacctttt ggcaaataaa ttttttaaaa
     8161 aaatttggat ggtattagcc gtccttataa ttgttgcagc tgtcttttcc agtgtatttc
     8221 gcgctcttac tccctgggct aaacaatata aaggggaggt ggaacagcat ttgacttcac
     8281 ttttgggtca acctgttact gttcaaacta tggaaacagg gtggtactgg tttgaaccag
     8341 tagtcaaatt aaaacatata agaattcatg atggtcataa taagtcaatt tatctggata
     8401 ggttaattgt cggtgttaat ttatttaaat ccctatggaa ttggcgcatt caacctggcg
     8461 tactttatat tgatgatatg catttggttt ttcgtgaaaa agacgggcat tggtgtattg
     8521 agggaatttc acgtaatgca atgaaagaca ctgatattac acctgaaaaa actagagaaa
     8581 tattagattg gctcgctcag caggaaaaat taattgtacg gcatgtttcc gctcacttac
     8641 attttaacga cgggtcatta atccctgtta acgaattaaa cctgtcgata gttaataagg
     8701 ggggaaatta taagataaag ggggaagcaa gactcgaaca aacaaatgca accgttttcc
     8761 aactgctagg gaatgtacaa ttcgatcctt ctcaaattaa gaacaccaat ggacgcttct
     8821 atttttcagc tattaatgtt ttacctgcac aatggcaaaa tttattgcca aaatcctcaa
     8881 tgcaactgga gggaggtaaa ggaaacgttt cattatgggt tgatttggag aaagggaata
     8941 tttcatcaat tcaggcagaa ttgagactta agcgtctggc atggaaatta ttaaataaaa
     9001 aaaatagtaa gttgatccaa tcattttatg ccaacatggg ctggaaattg gataaacaag
     9061 ggtggcagtt ccaggcagac catatgaaac taagagtagc aggggtcact tggccagaga
     9121 accaattgtc aattcgtttt aataaaaatc agaataccta tcaaatcttt acaaaatccc
     9181 ttattgtaga atcactatta tccgaagcaa ttaattggcc accggctatt caaaatgtac
     9241 tgcagttaaa accgcatgga atattgagtg atgttcaggt attaatcaaa gaaaatcaaa
     9301 ttaattatat tttaactcga tttgatcaat taggatggag agcaaaggat aatattcctg
     9361 gagtgagtaa tttatccggc gttttaaatt ggcagcctac ggaaggacgc cttgagttag
     9421 attcagagaa ttcaactttc gatatcaaag gttatcccgc tcaaaaactg accattttta
     9481 atggtgcttt tgattggaaa gagttaaatg atgggttgcg gttgagtatt gaacgcttgg
     9541 ttgcaagtca acccgaattg actattagtc tggaaggcgc tattgatcag ctgacccaaa
     9601 attctgtagg caatattcgt ttagtgggta atttttctgg gaaaaattta cagaagtgga
     9661 tgccttattt gcctaaaaaa cacatgaaag ccaagttatt tgcttggtta aataatgaca
     9721 tcaaacgttt agggcaagca acaggcaaga taactattaa tggaatggcg aaggattttc
     9781 catttgataa tggcaatggg gagttttcta tagtaagcca tgttgtcggc ggagatattt
     9841 taattactcc agaatggaga ttgattaaag atttggaagg ttatattaaa ctaaaaaata
     9901 gaaatcttga cattgatatc attgatggtg atgctcaagg tactcttctt aaacaaatga
     9961 atttgcgtat tgatgatatt ggcaaagata gggaaacctt attaattcat ggaattgttc
    10021 atgggaaagc acaacaaatg ctcaattttg ttatgaattc cccattaaag aaaaaattat
    10081 ctgctttaaa aatattatta ataaagggga tgctatcact caatttgcgt ttggaaatac
    10141 ctctttatcc agagaatgat gataatctgg cccgaggtga attaaccttt gaaaataata
    10201 taatcaacat caagcatcag atgggagatt tttctgtaag ggatgttact ggaaatttac
    10261 tgtttaatga aaaaggcatc caggatagtg ccttaactgc cagggcattc ggctaccccc
    10321 tgaatattaa aattcaatcg gaaaaatctc cacaaccatt aaccgcaata tcaattgaag
    10381 gtgaatgcac tgtagaatcc ctaaagaaaa aatggcactt acctattttt tctgtactca
    10441 aagggaatat tttagctaag gcattattaa aaattaccga cgatcctaat gatttggata
    10501 atatgaagtt gagttccgat ttgattggat tggcagttga tcttccagca cctttaggaa
    10561 aaacatataa agaaaaaacc cccttacagg ttaatctgga ctttaatcca caaaagactc
    10621 ttcgtttaag agttaattac aatggacgac taagtacgga tctattgatg caaaagcaaa
    10681 aaggcgtgtt taagttaaaa tcaggtcaaa tacgtttagg tagtgcgcaa gctgtaaatc
    10741 aggaccatcc tggcctcggt atagctggta ctttagacgg ctttgatttg caggcatgga
    10801 aagaggtaat aaacagattc tctaaagaga atgtgaaccc ctcactcttg agtttggtta
    10861 atattattga tgtaaaaatg ggtaaactaa actttttgaa tcagcagttt gataacatgt
    10921 ctttgtatgc gaaaaaacgc cctgataatg attggtcaat caatttaaaa cagaaaaaag
    10981 tcgatgcgga tttaacctac cattcggcaa ccaattctct gagtggcttt gtcaaatact
    11041 tacatttatc tgaaatcgga tcctcaaaaa ataataaaga tactccttca catactactc
    11101 ctgggcaaat ccccaatttg aatttaagag ttgataattt ttcaattgga aaaattcaaa
    11161 ttgggaatat cacccttaaa agtcattcta catcagaaca gtggaagatt gattattgca
    11221 ggatagactc accttattat caagctaatt ttgaagggga atggcttcag aaagggaagt
    11281 ttaatcaaac aaagctacag tctaaaatgt atttgaaaga tttggccaag agtcttgatc
    11341 gttgggcgat taatccagcg gtggatgcag ggaagggata tatagagttt catgggggtt
    11401 ggcaaggttc cttatatgat ttttctctgt ccaagttgaa tggttccatg tatttacagt
    11461 tgaaaaatgg ccgcattact catttgagcc cggagactga agagaaactt ggtttaggga
    11521 agttattaag catattgagt ttgcaaacta ttccaagacg gttaaaactt gattttagtg
    11581 atctctcaaa tgatggctac agttttgata tatttaaagg caactttaat gtcaataatg
    11641 gcattatgag caccaataac agttacattg atggcccagt agcttatgct agtatgaaag
    11701 gggatctgga tttggtaagg cgattatatg atttggattt aaacatttca ccgcatatca
    11761 ctgccagctt acccgttgta gctacgatag ctggaggtcc gattgctggt cttgctgcat
    11821 ggattgcaaa taaaattatt aatcaaagca tgcaaaaaat tgcgggatat agttataaaa
    11881 tttctggccc ctgggatcag cctattgtgc aacagcttaa tatagtgaag aaattgcctt
    11941 aactaaagga agacaagatt atttagtaaa cagttatgaa gtcacttagg gcctatggag
    12001 gaatataaaa tattctgcct tgttcagaga gtacttaagc ctaaagaaaa taatcaaaaa
    12061 ataattatga atacactaaa attacttttt tcttattact ggcgtctgag tctattgaag
    12121 gaaaccccgg agaattctcc tt
//
LOCUS       DACTJE010000095        12003 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.095, whole genome shotgun sequence.
ACCESSION   DACTJE010000095 DACTJE010000000
VERSION     DACTJE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.095"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1142)
                     /locus_tag="I9760_001942"
     CDS             complement(<1..1142)
                     /locus_tag="I9760_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5043706.1"
                     /translation="MSKVIHKLSLFLFKKFKIIWILLTIGILISAFYANWNINQKRAY
                     KDLKEVAINLSNNIDGFIEDLLQEIYALPVYGKKIVDCKSDLYPFLEHITLNNPKISG
                     VIISDNKHNLFCSTLPNNKDLIATPIRSRSIIGPYNLPLFDQPVYLIQQKMGNYLIGI
                     LVIASVLKSELQTDKNQSTSIALYNEYEKKNIFRIRYTEQNKNWVLSNTQETDTQYTP
                     LGLVAIEKLQSINGVSVVVSENNKTILHNFWYSQTITILIVLICSFILYALIKNMMTK
                     RYSLQGAIKLAIKNEEFYPVYQPLFDCDEERFTGVEVLLRWENEDSQIIMPDFFIAEA
                     EATGLIVPITLQIIEIAFKEFRSLLEERSYFHLAFNISALHFTDSV"
     gene            1279..2940
                     /locus_tag="I9760_001943"
     CDS             1279..2940
                     /locus_tag="I9760_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S13"
                     /protein_id="HAT5043707.1"
                     /translation="MEMRMMKRYSKRLLTYIYLLMKSIPLVFTMQQLSYSTDKIPIPT
                     NKLPDDLNKVMNKSIYKKALWGLRVVDLDTGNVLLNLNSSTQFYIGSVRKIFSVGELL
                     NERGADYQSVTRVHYDGVVHNGNLNGNLVLVASGDLTMGGRTLPDGHVAITEYDHNEA
                     NSLGNAIITQTNLLAGYQYLAKQVYKAGIRSISGDIVIDDRLFEPFNFRGEFDVTPIF
                     INDDVVDIVILPSLINENANVSWLPHSSAFTVINQLLTVNPHEKYNLELESKLPACIG
                     KPGCSGIIKGHLPINFIPPLTHNYPLVQTFRITKPSNYARTIFIEALQEAGINVSSID
                     KTKENPKKLLKSTNLYNKNNKIAALKSLPLGEHVKYILKVSYNIGADTSLVLYGLTKG
                     ARSMIESLNVEKKILDKKYGISPSEYHFIDGSGGGETKATNTAIIKWLTIMSKRPVFN
                     IFYNSLPILAVDGSLGSINNFQSDPSLSGATGRVHAKTGTYVLGNESGMLVKGRSLAG
                     YIISKKNHRLAFALVVNNIPIQSLEDLFAVAQDQGIIASILWRDY"
     gene            complement(3251..4585)
                     /gene="bioA"
                     /locus_tag="I9760_001944"
     CDS             complement(3251..4585)
                     /gene="bioA"
                     /locus_tag="I9760_001944"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAT5043708.1"
                     /translation="MVNTDQLIAKDLKHIWHPCSQMKDFETFPPIVVNKAQGSYLYTN
                     KGPVIDAISSWWCKSLGHGHPAVIAAIKNQLDCFEHVISANTTHPAAVELAEKLSEIT
                     GKQHVFFASDGSSAVEIAMKLAIHASQIKGHKNKNQFIALKNGYHGETIGTMSVSDLG
                     LYKTPYASFGVQCHYIDDIPYVSGLKDPLWTNCDAYWNKVVQQLNKICDKVCAILLEP
                     IIQGAGGMLCYSADFLSKISSWAKEKDIYLIADEIMTGIGRTGEWLACDHARIQPDLI
                     CLSKGLTSGSIPFSCVMIDSTLFDLFYDDYHTGKSFLHSHTYSGNPLAIVAALATIKT
                     IEQDNLLMHSQELGKIMFSYLQDIARDSRKLVNVRGVGAVVAADFIESSQARIGFKIY
                     QEAIKQGALIRPIGNTLYWLPPLNTDKEIIGKLAEITLNSIEKAYNKAQGYE"
     gene            4658..5605
                     /gene="bioB"
                     /locus_tag="I9760_001945"
     CDS             4658..5605
                     /gene="bioB"
                     /locus_tag="I9760_001945"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAT5043709.1"
                     /translation="MSEEKKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFA
                     TLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVEEVLQCAKEAKEGGAKRFCMGAA
                     WRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPS
                     YYDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPE
                     SVPINRLIPVEGTPLAQAERVEGIELVRTIATARILMPKSAIRLTAGRTEMSDELQAL
                     CYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMV"
     gene            5606..6751
                     /locus_tag="I9760_001946"
     CDS             5606..6751
                     /locus_tag="I9760_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAT5043710.1"
                     /translation="MTLNIKINEYTAQLQEQGLLRTRQILETDKIDYIRFDSNDYLSL
                     IEDRRIAEAYQKGYELYPCGSGASMVLSGYHSSHQTLERAFAEMLGVDDCLLFSSGYC
                     ANLAITALLGRLGTHCFIDKSVHASIYDGLALSRVTYSRYIHNDMNALSALLKSHLGD
                     SVLITEGIFSMSGQIAPLSTINTLCKENGSELFVDEAHAFGVIGPQGMGSVPGHGLTQ
                     NEVPLRVIPFGKAFASQGAVVGGRKDWINALLQTARSFVYSTAISPALCYGLLKTLEI
                     IAAADSRRAKLNQLVAQFKELIKSSPLNWLDSDTPIQQLRLGCPHKALHYASELKKDG
                     IYCSAIRAPTVNSASSGLRIIINYKHTIEQIIELFNKLHLIHEYSSR"
     gene            6732..7451
                     /locus_tag="I9760_001947"
     CDS             6732..7451
                     /locus_tag="I9760_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAT5043711.1"
                     /translation="MNIHLDKYGQGMPLVLFHGWGFDSQIWQPIIPYLKPKYQIILVD
                     LPGFGLTPMMDWESFKKNLLDQLPDKFALAGWSMGGLYATRLAIEEPARVQYLINITS
                     SPRFISDIDWPGVAEEVFVNFYNNLSKDINKTLKEFISLQLNKMKCDFKIGNPPSPEG
                     LAFGLEILGTWDFREQLKQISIPTVYLFGRLDPITPVKTMAIMEKNYPNFKYVLFNRA
                     AHMPFLSHTDLFITIMDEFIK"
     gene            7448..8086
                     /locus_tag="I9760_001948"
     CDS             7448..8086
                     /locus_tag="I9760_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAT5043712.1"
                     /translation="MKRYFVTGTDTDCGKTFVTNQLVNYFSDSAAIKPIASGCEYSEN
                     QLVNSDALLHQQQNHLPMEIVNPWRFRLPVSPHLSAREDGASIDVHKVADYCLNLQVN
                     DIKKLFIEGAGGLMVPLNEQDTWLDFLKLTRIPVILVVGMKLGCINHTLLSQEVLEIN
                     KIKCQGWIANCLDQDMLMLDENISTLEAKLNYPLLARTNYGGKISDICLSSL"
     gene            8221..8535
                     /locus_tag="I9760_001949"
     CDS             8221..8535
                     /locus_tag="I9760_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAT5043713.1"
                     /translation="MPIYEYQCTNCHHQFDLMQKVTDAPVKQCPKCYENTVTKLISPA
                     GFQLKGTGWYVTDFKNKGNGPKESTENKSGDPGINKENSSKETPAASSADSAKSTKGD
                     SE"
     gene            8532..9161
                     /locus_tag="I9760_001950"
     CDS             8532..9161
                     /locus_tag="I9760_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HAT5043714.1"
                     /translation="MKLISLRAYLITGLIVWLPIFVTIVVLRFIIDMLDSTLALFPKA
                     YQPEQLFGFYIPGFGVLFSLALLLVTGIIATNFLGQRLVSRGESLLAKIPLVRSIYNA
                     VKQVIHAVLSTNSQAFRKVVLVEYPRRGLWTIAFQTGSVNPEIKEKSKEDMMSVFVPT
                     TPNPTSGFMLMIPRQDAIELNMSIDEALKLVISLGVMQSDPALAQVKTQ"
     gene            9180..10961
                     /gene="aspS"
                     /locus_tag="I9760_001951"
     CDS             9180..10961
                     /gene="aspS"
                     /locus_tag="I9760_001951"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAT5043715.1"
                     /translation="MRTHYCSAVNELSLDKQITVCGWVHNRRDHGGVIFLDIRDRSGL
                     LQVVYEPENKEIFAIAEKLRSEFVVRVTGIVRKRPEGMINDKMETGRVEVIGTQLEIL
                     NQSPTPPFLPDDHQIINEDLRYKYRYIDLRRAVMQKKLTLRHKLNSCIRNYLNEQNFL
                     DIETPMLTKATPEGARDYLVPSRVHPGQFYALPQSPQLFKQLLMMSGFDKYYQIVRCF
                     RDEDLRADRQPEFTQLDIEMAFINEEDILQLIEGLLKIVFKEILNITLPDKLPRMSYK
                     EAMTRYGSDKPDLRNPLELIDIADLVKDCDFNVFASAANDNSGRVVALKLPNGCDLSR
                     KDLDNYGQFVTIYGAKGLAYIKVNDLSAGMAGLQSPILKFLSETAVHSILNRVEAQTG
                     DVIFFGADKAHVVNESMGALRNKLGHDRNLINSGWQLLWVVDWPMFELDPQSNKLQPM
                     HHPFTSPQELSAEALRSKPTQTLAKAYDIVINGYEIGGGSIRIHQPELQKTVFDLIGI
                     DEQEAHEKFGFLLDALQYGAPPHGGIALGIDRLAMLLTDSTSIRDVIAFPKTQTASCP
                     LTSAPSPAGNAQLTELGIRLAPTITTK"
     gene            10936..>12003
                     /locus_tag="I9760_001952"
     CDS             10936..>12003
                     /locus_tag="I9760_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HAT5043716.1"
                     /translation="MRQLLRQSNLNLSTTSFKYLWCFFLPLFLLFFSFSLFSASVKEN
                     NATLVEYLAHEKTHLTIAINEIKQKSAPKDEQEYNEKLRQIRTILSMNKAKIESLNSF
                     SENQKKEEVNLNDQLKRLQQMPLANAEVTIQEKVGKIETLLTVNKKTTELIAETLDLA
                     KQYQLLLNDVLNELKLWKANFDLKEKLKSIKAIRAKLNKELNILYENAVSSQDKKQPE
                     LTISEQIQYEANLYINNQRIAVIHQKLNALELQKKIIKTNILLLNNLDTKALQSAIDN
                     YREVINQYSAIESSLQTILNSLTNQANLFVEPGLQKSVSLLQNDVKTELKETSNQKLI
                     LSKNLEDYQNQLKKQISTRQSL"
BASE COUNT         3844 a         2052 c         2355 g         3752 t
ORIGIN      
        1 aataccgaat ctgtgaaatg tagtgcagaa atgttaaatg caaggtgaaa gtaagaacgt
       61 tcttctaata gagatctaaa ttctttgaag gcaatttcta tgatttgaag tgtaattggc
      121 acaattaacc cggtagcttc tgcttcagca ataaaaaaat caggcataat gatttgactg
      181 tcttcatttt cccatcgcaa taatacttca acccctgtga atcgctcttc atcacaatca
      241 aacaaaggtt gatataccgg ataaaattcc tcatttttaa tagccagttt tattgcgcct
      301 tgcaatgaat aacgtttggt catcatattt tttataaggg catacaaaat aaaggaacaa
      361 atcaaaacaa taagaatggt tattgtttga ctataccaaa agttatgaag gatggtttta
      421 ttgttttcag aaacaacgac agaaacacca tttatgcttt gtagtttttc tatagcaacg
      481 agacctaatg gggtatattg tgtgtcagtt tcttgcgtat tgcttaatac ccagttttta
      541 ttttgctcag tatatcgaat tcgaaagata ttttttttct catattcatt atatagggct
      601 atagaggtgg attgattttt atctgtttgt aattcacttt ttaagacaga agcaataact
      661 aaaataccta taaggtaatt gcccattttt tgctggatca gataaacggg ctggtcaaat
      721 aatggtaaat tataggggcc tataatagaa cgtgaacgaa tcggtgttgc aataagatct
      781 ttattattgg gtaaagttga acaaaataga ttgtgcttgt tgtcactaat aataacacca
      841 gagattttag gattattcag agtgatatgt tccaggaatg gatataaatc tgatttacaa
      901 tcgacaattt ttttcccata tacaggcaaa gcatagattt cctgaagtag atcttcaata
      961 aaaccatcta tgttgttact gagattgatt gcaacttctt ttaaatcttt ataagctctt
     1021 ttttggttaa tattccaatt agcataaaaa gctgaaatca ggataccaat agttaagaga
     1081 atccatatga ttttaaattt cttaaaaaga aataaagata atttgtgaat aaccttactc
     1141 atatccttga gctttgttat aggcttcttc tatagagttt aatgtgattt ctgctaattt
     1201 cccaataatt tttttatctg tgtaacaaca gttattatgc caaatttgac aaacctaaaa
     1261 gaataaaaac atatatcaat ggaaatgcga atgatgaaga gatactcgaa aagattactg
     1321 acttatattt atttacttat gaaaagcata cctctagtgt tcacgatgca acaactatca
     1381 tatagcacag ataaaatccc tataccaacc aataaactac ctgatgacct aaacaaagtg
     1441 atgaataaat ctatttataa aaaagcgttg tggggattac gtgttgttga tcttgatact
     1501 ggtaatgtat tgctgaatct caactcaagt acgcagtttt atattggttc tgtgcgcaag
     1561 atatttagtg taggcgaact attgaatgaa agaggagctg attatcaatc tgttactaga
     1621 gtccattatg atggtgtagt acacaatggt aatctgaatg ggaatctggt actcgttgct
     1681 tcaggtgatt tgaccatggg aggacgaact ttacctgatg gacatgtcgc tatcactgaa
     1741 tacgatcata acgaggcaaa ttcattgggt aatgcaatta ttacacagac taatctttta
     1801 gcaggttatc aatatctcgc taaacaagtt tacaaagctg gcattcgctc tatttcaggc
     1861 gacattgtga tagacgatag attgtttgaa ccatttaatt tcaggggtga atttgatgta
     1921 acacctattt tcataaacga tgatgtagtt gatattgtaa ttcttccaag tttgataaac
     1981 gagaatgcaa atgtatcgtg gctgccacat tcatcggcgt tcaccgttat caaccaactt
     2041 cttacagtta atccgcatga aaaatataat cttgaacttg agtcaaaatt acctgcctgc
     2101 ataggtaaac caggctgttc tggcatcatt aaaggccatt tgcctatcaa ttttataccg
     2161 ccacttactc acaactaccc attagtacaa acttttcgaa tcactaaacc atctaattat
     2221 gccagaacta tttttatcga ggcactacaa gaagcaggca tcaacgtttc atcgattgat
     2281 aaaaccaaag aaaatccgaa aaaactacta aaatctacta acttgtacaa taagaataac
     2341 aaaattgctg ctttgaaatc attgccattg ggagagcatg taaaatatat tctcaaagtt
     2401 agttataata tcggagcaga taccagcctg gtgttgtacg gactaacaaa aggcgcgcgc
     2461 tccatgattg aaagtctaaa cgtggaaaaa aaaatcctgg ataaaaaata tggcatatcc
     2521 ccatcagaat atcattttat tgatggcagc ggcggcggcg aaacgaaggc aaccaatacc
     2581 gctataataa aatggcttac tataatgtct aaaagaccag tatttaacat attttacaat
     2641 agtctgccta ttcttgcagt agatggttca cttggctcaa ttaataactt tcaatcagat
     2701 ccatcgcttt ctggggcgac aggtcgagtg catgcaaaaa ctggaactta tgtattggga
     2761 aatgagtcgg gtatgttagt taaggggcgc tctcttgctg gttacattat tagtaaaaaa
     2821 aatcaccgac ttgcatttgc cttagttgtt aataatatac ctatacagtc actagaagat
     2881 ttatttgctg tagctcagga tcaaggcata atagcatcaa ttctatggcg agattattga
     2941 ttattaatat aaataatatt tcattcaacg attgaaagac attatcgaaa tggagatgtt
     3001 tataaaataa acatttgtct aatttattgt ccactcaata atttaagtaa cttcgataaa
     3061 ctatattaaa tacacattgt atctcgctgt gtttaatttt tgtcgtcaac atagtgccca
     3121 gtttagattc aaaccaaaga agagttcggt tagcaggttt taggcaaatc acttggtttt
     3181 gggtcaagga gattgctgtt cttttaacaa ttggcgaacc gatttgtctt taaactattt
     3241 gtgaataacc ttactcatat ccttgagctt tgttataggc tttttctata gagtttaatg
     3301 tgatttctgc taatttccca atgatttctt tatctgtgtt aagaggtggt aaccaataga
     3361 gagtatttcc aattgggcga atcagtgcgc cttgttttat tgcttcctga taaattttaa
     3421 acccaattct tgcttgactg ctctctatga aatcagccgc aacaactgct ccaacaccac
     3481 gaacatttac aagttttcta ctgtcccttg ctatatcctg cagataggaa aacatgattt
     3541 taccaagttc ctgactatgc atgagtaaat tatcttgttc tattgtttta atagttgcta
     3601 aagcagcgac tatagcaagt ggattaccgc tataggtatg tgagtgcaaa aaagatttcc
     3661 ctgtatggta atcatcataa aaaagatcaa ataaggtact gtcaatcatg acacagctaa
     3721 atggtattga gccggaagtt aatcctttgg atagacaaat cagatcaggt tgtatccttg
     3781 catgatcaca ggctagccat tctccagttc tgcctatacc agtcataatc tcatcagcaa
     3841 ttaaataaat gtctttttcc ttagcccatg acgagatttt tgagagaaaa tctgcactgt
     3901 aacatagcat acctccagca ccctgaatga taggctccag aagtatcgcg caaactttat
     3961 cgcaaatttt attcaattgt tgtacgactt tattccaata agcatcgcaa tttgtccata
     4021 agggatcctt aaggcctgag acatagggaa tatcatcgat gtaatggcat tgcacgccaa
     4081 acgatgcata gggagtctta tagagtccca aatcactgac actcatggtg ccaatagttt
     4141 ctccgtggta gccatttttt aaggcaatga attggttttt atttttgtgc ccttttattt
     4201 gtgaagcatg aatagctaat ttcatggcaa tttcaacagc actggaacca tcactagcaa
     4261 aaaacacatg ctgttttcct gtaatttcgc ttaatttttc agctaattcc actgcagcag
     4321 gatgagttgt attggcacta atcacatgct caaagcagtc cagttggttc ttgatagcag
     4381 ctatcactgc tggatgacca tgccctaatg atttgcacca ccaactggaa attgcatcaa
     4441 tcacggggcc cttattggta tagagatagc tgccttgtgc tttgttgaca acgatagggg
     4501 ggaatgtttc gaaatctttc atttgtgaac aaggatgcca aatgtgcttt aagtcttttg
     4561 caataagttg atctgtgttt accattttaa aaattttggt tgacaagtat aggagcgact
     4621 ttatcatgtt cactattctg aattaaaggg ggtagccgtg tctgaagaaa aaaaacagtg
     4681 gtcaatttct gatattgaag caatttatca acaaccattt aatgatttgc tttatcaagc
     4741 acataccatt cataggacat atcacgatcc taattcttta caatttgcaa ccttgttaag
     4801 cattaaaacg ggcgcatgcc cagaagattg tggctattgc tctcaaagtg gtcactataa
     4861 aacgcatgta gaaaaagaaa aattgatgtc agttgaagag gtattgcaat gtgccaaaga
     4921 agcaaaggag ggtggggcaa aacgtttttg tatgggagct gcatggcgtt gtccacctga
     4981 taaagcaatt ccgcagctaa aagaaatgat agaaggagta aaatctttag ggcttgaaac
     5041 ctgtatgact ttaggcatgc ttaccaaaga acaggcctct catttaaagg aggctggatt
     5101 ggattattat aaccataata ttgatacctc accatcctat tacgataagg tggtaaccac
     5161 tcgaaaattc agtgatcgat tagatacttt aaataacgtt cgttcagcgg gaattaatgt
     5221 atgttgtggt ggtattttag gattggggga aaccagagaa gatcgaatcg aatttttatt
     5281 aacattagct aatatggaaa ctcctccgga aagtgtgcca attaatcgtt taattccagt
     5341 ggagggaaca cctttagccc aagctgagag agtagaaggg attgaattgg taagaaccat
     5401 tgctacagca agaattttaa tgcctaaaag tgctattaga ttgacagctg gtcgaaccga
     5461 aatgagcgac gagttacaag cactttgcta ttttgccgga gctaattctg tgtttattgg
     5521 cgataaatta ttgacagaag ataatcccca gcgcttcaaa gacaaaactc tttttaacaa
     5581 actgggtttg actgagatgg tataaatgac tcttaatata aaaattaatg agtatacagc
     5641 acagctgcaa gaacaaggat tattgcgtac aagacaaatt ttagaaacgg ataagataga
     5701 ttatattcga tttgattcta atgattattt gtcgctcata gaagataggc gaattgcaga
     5761 agcctatcaa aagggatatg agttgtatcc ttgtggaagt ggtgcttcga tggttttaag
     5821 tggctaccat agtagccacc aaacccttga gagggccttt gccgaaatgc tgggagtaga
     5881 tgattgtctt ttattttcct ctgggtactg tgcaaatttg gccattacgg ccttgcttgg
     5941 gcgtttggga acacattgtt ttatagataa aagcgttcat gcatcaattt atgatggatt
     6001 ggctttgtca cgagtaacct actctcgtta catacataat gacatgaatg ctttgtctgc
     6061 cttgttaaaa tctcatttgg gcgattctgt tttgattaca gaaggtattt ttagtatgag
     6121 tgggcagata gcgccgttat caacgattaa tacgttatgc aaagagaatg gctctgagct
     6181 ctttgtagat gaagcgcatg cttttggtgt gattggaccg caaggaatgg gctctgtgcc
     6241 aggccatggg ctgacacaga atgaagttcc cttgcgagtt attccttttg gtaaggcatt
     6301 tgcttcccag ggcgctgtgg ttggtgggcg gaaagattgg ataaatgctt tgttgcaaac
     6361 ggcacgttct tttgtttatt caactgctat tagtcctgct ttatgctatg gtttgctcaa
     6421 gacattggag ataatagcag ctgccgactc cagaagagca aaattaaacc aattagttgc
     6481 tcaatttaaa gaattaataa agtcttcgcc cttaaattgg ctggattcag atactccaat
     6541 tcagcaatta aggttaggat gccctcataa ggcactgcat tatgccagtg aactgaaaaa
     6601 agatgggatt tattgctctg ctatcagagc tccaacggta aatagtgctt cttctggttt
     6661 acggattatt attaattata agcatactat tgaacaaatc attgagttat ttaataaatt
     6721 gcacttaata catgaatatt catctcgata aatatgggca aggtatgcct ttggtcctat
     6781 ttcatggctg gggatttgat agccaaatct ggcaaccgat aattccttac cttaagccta
     6841 aatatcagat tattttagtc gatttgcctg gttttggatt gactccaatg atggactggg
     6901 agagttttaa aaagaatttg cttgaccaat tacctgataa atttgcattg gcagggtggt
     6961 caatgggtgg gctgtacgcg acacgtttag caattgaaga gcctgccaga gtgcagtatt
     7021 tgataaatat aacgtcttca cctcgcttta tctctgatat tgattggccg ggagtagcag
     7081 aggaggtatt tgtcaatttt tataacaatt tatcgaaaga tattaataag acattaaaag
     7141 agttcatatc cttgcaactc aataagatga aatgtgattt taaaataggt aatcctccat
     7201 ctccagaagg cttggcgttt ggtctcgaaa ttttgggtac ctgggatttt agagagcagt
     7261 tgaaacaaat atctatacct actgtttact tatttggtcg acttgatccg attacacctg
     7321 tcaagaccat ggcaattatg gagaaaaatt acccgaattt taagtatgtc ctcttcaaca
     7381 gagcagctca catgcctttt ctgtcccata ccgatttatt tattacaata atggatgaat
     7441 ttataaaatg aaacgatatt ttgttactgg tactgacact gattgtggta agacatttgt
     7501 aaccaatcaa ttggttaatt atttttcaga ttcagcagcg ataaaaccca tagcaagcgg
     7561 atgtgagtat agtgaaaatc aattagtaaa ttctgatgca cttttacacc agcaacagaa
     7621 tcatttgcct atggaaattg ttaatccctg gcgatttaga ttgccagttt cgccgcactt
     7681 atcagcaagg gaggatggtg caagtattga tgtccataaa gtggctgatt attgccttaa
     7741 tttgcaagtg aatgacatta agaaattatt tatcgaagga gccgggggat taatggttcc
     7801 tctgaatgag caggacactt ggcttgattt tttaaaatta accaggatcc ctgttatttt
     7861 agttgtaggc atgaagttgg gttgcatcaa ccacactttg cttagccagg aagtgctcga
     7921 aataaataaa atcaaatgtc aaggatggat tgctaattgc ctggatcagg acatgctaat
     7981 gcttgatgaa aatattagta cccttgaagc caaattaaac tatcctttat tagccaggac
     8041 aaactatggc ggcaaaattt ctgatatatg tttaagctcc ttgtaatcgc agtgaaattt
     8101 tatatcaaaa cagcactata tacctgatct tactgcttgt tctgcttgcg ctatacccat
     8161 ggcagcaatt ataatgaaaa gttaaattac agatacttcg agtttatgga gtgatgctag
     8221 atgccaattt atgaatatca gtgcacaaac tgtcatcatc aatttgattt gatgcaaaaa
     8281 gttacggatg cgcctgtaaa acaatgccca aagtgttatg aaaatactgt aacaaaactt
     8341 atatctccgg ctggatttca gttaaagggg actggctggt atgtcacaga ttttaaaaac
     8401 aaaggtaacg gaccaaagga gtcaactgag aataaatcag gagatcccgg catcaataaa
     8461 gagaattctt ctaaagaaac tcccgcagca agctctgcag atagcgcaaa atcgacaaaa
     8521 ggtgatagcg agtgaaatta atctccctca gggcctattt aattaccggg ttaatagtgt
     8581 ggttaccaat atttgtaacc attgtagtcc tgcgatttat tattgatatg cttgatagca
     8641 ccttggcttt atttccaaaa gcttatcaac ctgagcagtt attcggtttc tatataccag
     8701 gctttggcgt tttattttca ttggctttat tactcgtgac aggaattatt gcaaccaatt
     8761 ttttggggca gagacttgta agtcggggtg aatccttatt ggctaaaata cctctggtgc
     8821 ggtcaattta taatgcagta aagcaagtta ttcatgctgt tctatcaact aatagccaag
     8881 ctttccgcaa agttgtttta gttgagtatc ctcgtagagg actttggacc atcgcatttc
     8941 agactggctc cgttaatcct gaaattaaag aaaaatccaa ggaagacatg atgtctgtat
     9001 ttgttccaac tacacctaac cctacttcgg gttttatgtt gatgataccc aggcaagatg
     9061 ccatcgagtt gaatatgagc attgatgaag cgttaaaatt agtaatatcg ttaggtgtaa
     9121 tgcagtctga tcctgcattg gcacaagtaa aaactcaata aactttaata ggtggtttta
     9181 tgcgtacaca ctactgttcg gccgtgaatg aattaagctt ggataagcaa atcacagttt
     9241 gtgggtgggt gcataataga cgagatcatg gcggagttat ttttttagac attcgtgatc
     9301 gttccggatt attgcaagtg gtgtatgagc ctgaaaataa ggaaattttt gcaattgctg
     9361 aaaaactaag atcagagttt gttgttcgtg ttactggcat agtccgtaaa agaccagaag
     9421 gaatgatcaa tgataaaatg gagactggca gggttgaagt aatcggtaca caacttgaaa
     9481 ttcttaatca atctccaact ccaccgtttt tgccagatga tcatcaaatt atcaatgaag
     9541 atttgcgcta taaataccgc tatattgatt tgcggcgtgc tgttatgcag aagaaactga
     9601 ctttaagaca caaattaaac agttgcatac gtaattactt gaatgagcag aattttttag
     9661 acatagaaac gcctatgtta actaaggcaa ctcccgaagg cgcgcgggat tatcttgtgc
     9721 cttcaagggt gcatccaggg cagttctatg ctttacctca atcgccacaa ttatttaagc
     9781 aattgttgat gatgtcaggg tttgataaat attaccagat tgtccgctgt tttagagacg
     9841 aagatttaag ggctgacaga cagccggaat ttacgcagct tgatatagaa atggcattta
     9901 ttaatgaaga agatatattg caactaattg aaggattgct caagattgta tttaaggaaa
     9961 tccttaacat taccttacct gataaattgc caagaatgtc gtataaagag gctatgactc
    10021 gctatggaag tgataaacct gatctgcgta atccactgga gttgattgat attgcagatt
    10081 tggtaaaaga ttgcgacttt aatgtatttg cctctgctgc gaatgataat tcaggaagag
    10141 tggttgcttt aaaactacct aatggatgtg atcttagtcg aaaggatctg gataactatg
    10201 gacaattcgt aactatatat ggggctaaag gacttgcata tattaaagtc aacgatttaa
    10261 gtgctggtat ggcgggttta cagtcaccaa ttctaaagtt tttatctgaa acggctgtgc
    10321 actctattct taatcgggtt gaggcgcaga ctggcgacgt catttttttc ggtgctgata
    10381 aagctcatgt agtaaacgaa tctatgggag ccttaaggaa taaactgggt catgatagaa
    10441 atctgattaa ttctggatgg caattactgt gggttgtaga ttggcctatg tttgaattgg
    10501 atcctcagag caataagtta cagcctatgc atcatccctt tacatcccct caggaactat
    10561 ctgccgaggc acttcgctcc aagccaacgc aaacattagc caaggcatat gatatagtta
    10621 ttaatggtta tgaaattggc ggtggatcta ttcgtatcca tcaacctgaa ctacaaaaaa
    10681 ctgtatttga tttgatcgga attgatgagc aagaggcaca tgagaaattt ggatttttac
    10741 tcgatgcctt acaatatggt gctccacctc atggtggaat tgcgttggga attgatagat
    10801 tagctatgct gctgacagac tcaacttcca taagggatgt gattgcgttt cccaaaactc
    10861 aaaccgcatc atgtccttta acaagtgcgc catcgcctgc gggcaatgca caattaactg
    10921 aactgggtat aagacttgcg ccaactatta cgacaaagta atttaaattt aagcaccaca
    10981 agttttaaat acttgtggtg ctttttcctg cctctgtttt tattattttt tagtttttca
    11041 ctcttttcag catcagttaa agaaaataat gccacgctag tagaatattt ggctcatgaa
    11101 aaaactcatt tgactattgc tatcaatgaa attaagcaaa agagcgctcc caaggatgag
    11161 caagagtaca atgagaagtt aagacaaatt agaactattc tttcaatgaa taaagcaaaa
    11221 attgagagtt tgaatagttt tagtgaaaat caaaagaaag aagaagtcaa tttgaatgac
    11281 caactaaagc gtttgcaaca aatgccactt gccaatgctg aagtaaccat tcaggaaaaa
    11341 gtaggtaaaa tcgagacctt gcttactgtc aataaaaaaa ccactgagtt aattgcagaa
    11401 actttggatt tggccaaaca atatcaatta cttttaaatg atgttttgaa tgaattaaaa
    11461 ctttggaaag ccaacttcga tttgaaagaa aagcttaagt caattaaagc cattagagct
    11521 aaattgaata aagagctgaa tatcctttat gagaatgcag tatccagcca agataaaaag
    11581 caaccggaac taacgattag cgaacagatt caatatgaag ctaatttgta catcaataat
    11641 caaagaattg cagttattca tcaaaagttg aatgcattgg agttacagaa aaagataatt
    11701 aaaacgaata tcctgttatt aaataatttg gatactaaag ccttgcaatc agcaattgat
    11761 aattacagag aggttattaa tcaatattcg gctatagaga gttctttgca aacaattctg
    11821 aattctctta caaatcaggc caatcttttt gttgagccag ggttacaaaa atccgttagc
    11881 ttattgcaga atgacgttaa aacagaatta aaggagacct ctaatcaaaa acttatctta
    11941 tccaaaaatt tagaggatta tcaaaatcaa ttaaagaaac agatttcaac aagacaaagc
    12001 tta
//
LOCUS       DACTJE010000096        11687 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.096, whole genome shotgun sequence.
ACCESSION   DACTJE010000096 DACTJE010000000
VERSION     DACTJE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11687
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.096"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..394
                     /locus_tag="I9760_001953"
     CDS             <1..394
                     /locus_tag="I9760_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAT5043717.1"
                     /translation="INQIAVLTLISLIMTIGVYGLVACIVKLDDLGFILREKKHPLKQ
                     IGILLIGAAPILMKILSVAGTIAMFLVGGGIITHQITAIHHFTENLSAMMSIGVDFFI
                     GIISGAITAALYFTFKKMKSKFKLMNTK"
     gene            562..1017
                     /locus_tag="I9760_001954"
     CDS             562..1017
                     /locus_tag="I9760_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HAT5043718.1"
                     /translation="MPDITHYLKPMVLFSGLLVATFAMAEATKAEETSTVKLSTILQQ
                     LSDQGYKNIHDAEIDDGVLTVEGSNEQGVKFEIHIDPATGKILKKQEHPQPKYSIIDI
                     TKKIENEGKFKIIEIEFDEGIYKIKGETASHEKKKLYIDPNTGEAKTNH"
     gene            complement(1098..1874)
                     /locus_tag="I9760_001955"
     CDS             complement(1098..1874)
                     /locus_tag="I9760_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5043719.1"
                     /translation="MSKTIYFFSNNLKLEGKLEEPTGQCLGYVLFAHCFTCGKDIAAA
                     SRIASALVANGFAVLRFDFTGLGSSEGSFSETNFSSNVEDLVAAADYLRTHYQAPVLL
                     IGHSLGGAAVLLAAKKITEVKAIATIGAPASAHHVKHHFSADLSKIESDGEAHVTLGP
                     RSFTIKKQFLQDIDRYQETIKSDAGKALLIMHSPIDKVVSIKEAEKIYKAAQHPKSFI
                     SLDKADHLLSNKRDSQYAAEVIAAWASRYLAPSLEDKRAE"
     gene            complement(2377..2658)
                     /locus_tag="I9760_001956"
     CDS             complement(2377..2658)
                     /locus_tag="I9760_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SapA"
                     /protein_id="HAT5043720.1"
                     /translation="MLICKTQITQDWVEFADKATKFAWQFGIGSLYALSDSLLIDINY
                     NFISIGEVRNSGQYNAIAANNTPASGAPTKFTNVYSNQAEIGIHYQFNT"
     gene            2870..3289
                     /locus_tag="I9760_001957"
     CDS             2870..3289
                     /locus_tag="I9760_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAT5043721.1"
                     /translation="MKTYRKELWFNIPERMGFINITDKVIECLKESRIQEGLILVNAM
                     HITASVFINDDESGLHQDYKKWLERIAPHQPIEQYRHNDTGEDNADAHIKRQIMGREV
                     VVAITEGRLDFGPWEQIFYGEFDGHRDKRVLVKIIGQ"
     gene            complement(3543..4520)
                     /locus_tag="I9760_001958"
     CDS             complement(3543..4520)
                     /locus_tag="I9760_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043722.1"
                     /translation="MNLKKEVFLNQNCAEMMIKKAARLILGSDLDFEYTRDVQDIQID
                     LGPAFMFSPDEEKTLWVSGKNQETLERDLATLNKSSAYFFRTGTQGGAGHWQVLYYEA
                     AKSGWVSYSSQSNHFQVTDSNGKLTASGKGLLVPHANWGKENGNYAFLLVNASAQNII
                     HAANFVYIFRTQNEDAAIQYCALNQALHPEIKRITITKVQTQTRLTPEIHCPRGDLSY
                     ANLKNVYSKHLALLHVINRLEAGSKSYNPYWMGSQEKLNKIFEALGHCYQKGLDVDNE
                     LNNSNSKLSKAINMRRLPSLFSFWCEKNESFVESRKIIEEHRICRLNNL"
     gene            4741..5220
                     /locus_tag="I9760_001959"
     CDS             4741..5220
                     /locus_tag="I9760_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043723.1"
                     /translation="MKEKTAKQLKLFIIYLLSYWLLSIISCLIAFGYDDSLRMLLASP
                     KSDLSGALLFFSSFIAAALLFVFRYKTFSDKPYPYFIFGFYVGNVSLLMLFILDAFIR
                     ELIVWKFPEFLLVFISPFVELVLSYLFFGFAFLAIIPAVTSAFILYAVQRKLLLSPI"
     gene            5395..5607
                     /locus_tag="I9760_001960"
     CDS             5395..5607
                     /locus_tag="I9760_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParD-like family protein"
                     /protein_id="HAT5043724.1"
                     /translation="MGIVKISDELHEATKLMARAMSRSINSQAEYWIRIGKLAEENPS
                     ITYTDILKILVHQASLGEIYDVSEDA"
     gene            5588..6349
                     /gene="map"
                     /locus_tag="I9760_001961"
     CDS             5588..6349
                     /gene="map"
                     /locus_tag="I9760_001961"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAT5043725.1"
                     /translation="MLVKTPEEIKKMRVAGQLAASVLEMIEPYVIAGTSTKELEQICR
                     QYIVEDLKAIPSTLNHYGFPACICTSINHVVCHGIPSEKKLKNGDIINIDVTVQKDGY
                     IGDTSKMFLVGNIKPFAKKLVEVTQECLYKAISIVRPGAHLGDIGNIIQTHAEQHGYS
                     IVREFGGHGIGKSMWEDPHIMHFGKPNTGLRLQAGMTFTIEPMLNLGRKEVKTLGDGW
                     TVVTKDHKLSAQWEHTILVTDNGHEILTLRADEHL"
     gene            6454..7536
                     /locus_tag="I9760_001962"
     CDS             6454..7536
                     /locus_tag="I9760_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5043726.1"
                     /translation="MPCEKCDSAATYHLSGTYQLLILPPRGHCYSKLLKHLTENNFDI
                     NKRDSGIIELLFTAERAQDIGHALERCYSQAELEDSKAVLLPIDIADRNVERVLNHTH
                     SLKKIVGLCLSQWLVGLIENQGLSTFCQPILHNTTLKPYGFECLLRGKQDNNILPSSD
                     LFGAAKSSDMLFLLDKKARICHIENMSKIDIADKKIFINFNPTAIYDPLFCLTTTNQS
                     LKKTSIKPAQIVFEVTESDEVKDKKHLLKIIDYYRNQGYCVALDDLGSGYSSLNLLTE
                     LQPDYVKLDQELISQIHTNEFKQIILEKICEMATKLKIKIICEGIETEDELQIIKNYA
                     IDFYQGFLFEKPMPYEKIPDYIKASA"
     gene            7566..8444
                     /locus_tag="I9760_001963"
     CDS             7566..8444
                     /locus_tag="I9760_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT5043727.1"
                     /translation="MLTNIERAKLQLIDETNDMVIITKAEPLDAPNGPEVVWVNKAFE
                     NISKYKANEIIGQTPRILQGKETNQNTLKMIKEALKAQTNINVDLLNYTKDGTPYWIS
                     FSIVYLRDGQGNLCYLGAIEKDITALKNLNFKLSEKAKTDPLTNVYNQENFFIAGNEA
                     IKNFHQNQEAVGLLFFDVDDFKGINDTQGHIYGDMILKKVAQESLKLVRKTDNVFRYG
                     GDEFAIIFSGIDVKILERKAQQLQKKLAKSHISVSIGGTISIQSDKSINTLLARADEA
                     LYYVKANKKGLFFIAE"
     gene            complement(8791..9537)
                     /locus_tag="I9760_001964"
     CDS             complement(8791..9537)
                     /locus_tag="I9760_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043728.1"
                     /translation="MKRYIWFHPSYVLESTEATLLGQQTNAKDHGYILLNDQELGKVT
                     CEDHLTIMGHSTLAPTPAEDTGLYIQGDTADDLIRRLTTNGLKEAPKILSLESCRAGI
                     SGGLAQQLSRSPFFKYTLIEANLNSVGRKPMEMQWNFPTDSLGRAVLKLDNSPWMFYL
                     GGFLVGRLTHDAYTTEDLFSLLSPPTFHKHFFANYQPGFFGGRSGRHCLFNKTTITLE
                     KASNFALEDQDSASAKALEVVLKKLYSQPA"
     gene            complement(9798..10240)
                     /locus_tag="I9760_001965"
                     /pseudo
     CDS             complement(9798..10240)
                     /locus_tag="I9760_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212845.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10458..10772
                     /locus_tag="I9760_001966"
     CDS             10458..10772
                     /locus_tag="I9760_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043729.1"
                     /translation="MKPTLFLVLMWICAESFGATIMVKGLPSPLGYSNDLYYWPPNVA
                     ISPDTTNLFITMDGINKVCFLNTGLQGGLEQISEISIIINGVKKDWNCFPYTTTILEA
                     RP"
     gene            11087..11311
                     /locus_tag="I9760_001967"
     CDS             11087..11311
                     /locus_tag="I9760_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043730.1"
                     /translation="MKPMKNSKEQEHIAPEAQDPRLSYTEDGRRPLTSTTSAKHPNSL
                     FAKLEERSARESYDQFGNNVTMHWTKDPWN"
BASE COUNT         3779 a         2240 c         2140 g         3528 t
ORIGIN      
        1 gattaaccaa atagcggtat taactttgat ttcattaatt atgaccattg gagtttatgg
       61 tttggttgcg tgcattgtca aattggatga tctgggcttc attttacggg aaaaaaagca
      121 cccacttaaa caaattggaa ttcttttgat tggagcagcc ccaatactta tgaaaatttt
      181 aagtgtggca ggaacaattg ccatgttttt agtaggaggg gggattatta ctcatcaaat
      241 aactgccatt catcatttca cagaaaacct gtctgcaatg atgtctatag gagttgattt
      301 ttttatagga attatttcag gtgcaattac agctgctcta tattttacat tcaagaagat
      361 gaaatcaaaa tttaaactaa tgaatacgaa gtaagtacta taagagtcaa aatccattgt
      421 aaaatcaaag taactttgca ttccatcgct caaatgacta tagttaaaca ataatttttt
      481 aacaccaaac ctcatcaggc gattcttttt ttgcgattta tttgaatttt aataccatac
      541 tatcaatagg agataagcag gatgccagat attacacatt accttaaacc catggtttta
      601 ttttcaggac tattggttgc tacttttgcc atggccgaag caacaaaagc tgaggaaact
      661 tctactgtaa aattaagtac tattcttcaa cagttgtctg accaggggta taaaaacatt
      721 catgacgctg aaattgacga tggcgtattg actgttgaag gcagcaatga acaaggcgtt
      781 aagtttgaaa ttcacatcga tccagctact ggcaagattt taaaaaaaca agaacatcca
      841 caaccaaaat attccatcat agacattacc aaaaaaattg aaaatgaggg taaatttaaa
      901 atcattgaaa ttgagtttga tgaaggtatt tataaaataa aaggtgaaac agccagccat
      961 gaaaaaaaga agctatatat cgaccccaat accggggaag ccaaaacaaa tcattaatcc
     1021 catataaagg ggcaagcctt tcttttttcc ttagtatgcc caattattct ccctagagga
     1081 aaggaagaga gctgtttcta ctcagctctt ttatcctcta atgaaggagc taaataacgg
     1141 ctggcccagg cagcaataac ctctgcggcg tattgtgagt cccttttgtt acttaataaa
     1201 tgatcagcct tatcaaggct gataaaactt ttgggatgtt gggcggcttt atagattttt
     1261 tctgcctctt taatggatac cactttatct ataggagagt gcataatgag taaggcttta
     1321 ccagcgtcgc ttttaatcgt ttcctggtat ctgtcaatat cttgtagaaa ttgtttttta
     1381 atagtgaatg agcgtgggcc cagggtaaca tgagcttcgc catcagattc tattttactt
     1441 aaatcagcag aaaaatggtg tttgacatga tgagcactgg caggagcacc aatagtagct
     1501 attgccttta cttcagtaat tttcttggcg gcaaggagca cagccgcacc accaagacta
     1561 tggcctatca aaagaacagg tgcttgataa tgagtcctta aatagtcagc ggcagcaact
     1621 aaatcttcaa catttgaaga aaaattcgtt tctgaaaagg aaccttcgct gctacctaag
     1681 ccagtaaaat caaaccgtaa caccgcaaat ccattagcaa ccaaagcgct ggcaattctg
     1741 cttgcagcag ctatatcttt tccgcaggta aaacaatgag caaataaaac ataaccaagg
     1801 cattgacctg ttggttcttc cagttttcct tccagtttca aattgttact gaaaaaatat
     1861 atagtcttag acattatgct ttccttgttt gtcttgattg agatgctaac aagaaacatt
     1921 caaatctgtt tatcaatcca ttttcttact ctaaaatcaa ttcaatttat tcctactgta
     1981 gagtttggat attctggaag tgttcacata ctaacaaata tagacacatt taagccaact
     2041 tcccaattta tttggaatat ttattaacct tatagctatg atgtagcgaa acgcaatcaa
     2101 ggacaacgat ttatatacat tacaatctac ttaaagcacc gctcgtttaa catcatggaa
     2161 gattgactgc ccactttttc catcatgagg taaagtccgt aaaattacgg gaaaatttgc
     2221 caggaaaact atgagtgaag taccaggcag ctaaaaacca ggaaagacct gagtacgaag
     2281 gtagttttca tttcagccga gtagatagca atgggcaggt taaacagtgg ctcccattga
     2341 tgtcatcttt ttacttcaac ctgccttgaa ttggcgctaa gtattaaatt ggtaatgaat
     2401 accaatttca gcctggttgc tatacacgtt ggtgaatttt gttggcgcac ctgaagctgg
     2461 agtattattg gctgcaatag cattatactg acctgaattc ctcacttcgc caattgaaat
     2521 aaaattataa ttgatatcga ttaacaacga gtcactgagg gcatagaggc ttccaatacc
     2581 aaactgccag gcaaattttg tggctttgtc agcgaactct acccaatctt gtgttatctg
     2641 ggttttgcaa atcaacaata atataattga cccgaataca ttaaggcatt tgcattttta
     2701 ccataagcct aaaatgcctc aatggattgt tctctccaca accccatgat tcatgcctta
     2761 agacccagaa gagttctcta ataaagatta aattgtcatc catttgaaat aaaaccagga
     2821 atgctatatg ttgccagtta ttgatttatg cccaaatcat tgtcaaaaca tgaaaacata
     2881 ccgaaaagaa ctatggttta atatcccaga gcgtatggga tttatcaata tcacagataa
     2941 agtcattgaa tgcttaaaag aaagtcgtat tcaagagggt ttaatattgg ttaatgccat
     3001 gcacatcacc gcttctgtat tcatcaatga tgatgaaagc ggcttgcatc aggattataa
     3061 aaaatggctt gagcggatag caccccatca acccattgag caataccgcc ataatgacac
     3121 tggtgaggat aatgctgatg cccatatcaa acgccaaatc atggggcgag aagtggttgt
     3181 cgctattaca gaaggaagac tggattttgg tccatgggaa caaatattct atggtgagtt
     3241 tgacggccac cgtgataaaa gagtcctagt aaagattatc ggtcaataaa gcatgacttg
     3301 aatggatcat gagtaattaa agcataaaag gtccggtttt tcctttttcc ttaataagct
     3361 ctgtgcccta attcgcgcca aagaaaacgg aaaatcctgc ccatgatcta aaatttaact
     3421 ggagcctctg gctccattag atgttactgc agactccatt tgagatagta atgaccgtta
     3481 actgttatat cctatacaac atcaacaaca tgagaggtca tgccataaac tatacacagc
     3541 ttttataaat tatttagcct gcaaatcctg tgttcttcaa ttatttttct tgattccacg
     3601 aaagattcgt ttttttcaca ccaaaaacta aaaagcgatg ggaggcggcg catattgatt
     3661 gctttggata atttgctgtt gctattgttt aattcattat cgacatcaag gcctttttga
     3721 taacaatgtc caagtgcctc aaaaatttta ttgagcttct cttgactacc catccagtaa
     3781 ggattgtagc ttttacttcc tgcttccagg cggttgataa catgcaacaa ggctagatgc
     3841 ttactgtata catttttaag attcgcataa gataaatctc cccggggaca atgaatctct
     3901 ggtgttaacc tcgtttgtgt ttgaactttg gttatagtta ttctctttat ttctggatga
     3961 agcgcctgat tgagcgcaca atattgtatt gcagcatcct cattctgagt tctgaaaata
     4021 tacacgaaat tcgctgcatg aatgatgttt tgtgcacttg cgtttacgag caaaaatgca
     4081 taatttccat tttcctttcc ccaattggca tggggaacaa gcagtccctt accactggct
     4141 gttaacttcc cattgctgtc agtcacttga aagtgattgg attgagatga atagctaacc
     4201 caacctgatt tagcagcctc atagtacagg acttgccaat gaccagcccc tccttgagta
     4261 ccagtccgga aaaagtaggc actgctttta ttaagagtcg ccagatccct ttccaacgtt
     4321 tcctgatttt tcccactaac ccacagtgtt ttttcttcgt ctggactgaa cataaaagca
     4381 gggcccagat caatttgaat atcttgaaca tctcgagtgt attcaaaatc aagatcactg
     4441 cctaaaatca accgagcagc tttctttatc atcatttctg cacaattttg gtttaagaac
     4501 acttcttttt ttaagttcat tggaaaatcc cagtcatctt tcgattatta taatacaaaa
     4561 taatcacata aaaagctaag tgattgcttc gattataaag ccatcctaat atttgagtcc
     4621 cccttaagac agcagtctgt ggccccgttc aggagcatgc ccatgcatca atgcccaaag
     4681 agtcgtgtta aaatcccaaa gatagaagcg ccgtttaaaa cagcttgatt aaaattggtt
     4741 atgaaagaaa agaccgcgaa acaattgaaa ttgtttatta tctatctact tagctattgg
     4801 ttattaagca ttatttcatg ccttatcgct ttcggttatg atgattccct acgcatgttg
     4861 cttgcctcgc caaaatcgga tctcagcggt gctttattat ttttctcttc atttattgcc
     4921 gccgctttgt tgtttgtttt tcgatacaaa actttttcag ataaacctta tccttatttt
     4981 atttttggat tctacgtagg aaacgtctcg ttactgatgt tattcatatt ggatgctttt
     5041 attcgcgaac tcattgtatg gaaattccct gaattcttac ttgtttttat atcaccattc
     5101 gttgaattag tgctctccta tctatttttt ggctttgcat tcctggcaat cattccggca
     5161 gttaccagtg cttttattct ctatgcagtt cagagaaaat tattattgtc accaatataa
     5221 acctccagag tcttctcttt ttcatttgta atcgctccct tattctcgcg ttggaaaaaa
     5281 ggaagcctgc ccttaatcta ttgcccgatt tatttattcc ccaaaaaata tattttataa
     5341 tgaaatgtat cattttatat taaaacgcta tattatacat tttttggaga aaatatgggc
     5401 atagtaaaaa tttcggacga attgcatgaa gcaaccaaac tgatggcaag agccatgagt
     5461 cggtcaatca attcacaagc agaatattgg attagaatag ggaaattggc agaagaaaat
     5521 ccctccatta cctatactga cattcttaaa attttagtcc atcaagcttc actaggtgaa
     5581 atatacgatg ttagtgaaga cgcctgaaga aataaaaaaa atgcgcgttg ctgggcagtt
     5641 ggccgcatct gtacttgaaa tgatagagcc ttatgtcatt gcaggtacca gtacaaagga
     5701 gcttgaacaa atttgtcgtc agtatattgt cgaggatttg aaagcgattc cctcgacgtt
     5761 aaaccactat gggtttcccg catgcatttg cacttcgata aatcacgtgg tatgccatgg
     5821 catcccctca gagaaaaaac ttaaaaatgg cgacatcatt aatattgatg tgactgtgca
     5881 aaaggacggt tatattgggg ataccagcaa aatgtttctc gtcggcaaca tcaagccatt
     5941 tgctaaaaag ttagtggaag ttacccagga atgcctatac aaagctattt caatagttcg
     6001 cccaggggct catcttggag acatcggaaa cataatccag acacatgcag aacaacatgg
     6061 atattcaata gtacgagagt ttggtgggca tggaattggt aaatccatgt gggaagatcc
     6121 tcacattatg cacttcggta aaccaaatac cggattacga ttacaagcag gaatgacttt
     6181 cactattgag ccaatgctca atttgggccg taaagaagtc aagactttag gggatggttg
     6241 gactgttgtc acaaaagacc ataaattatc tgcacaatgg gagcatacca ttttagtgac
     6301 cgataatgga catgaaatac tgaccttaag agcagacgag cacctataga catatctcct
     6361 cttgcttggt aattactgtt cctccaagcc tcgcgacgga aaacatatac tataatcaaa
     6421 ctaaaggaaa cccagtcaat caagggaaat agcatgcctt gcgaaaaatg cgattcagcg
     6481 gcaacctacc atttatcggg aacctaccag ttactcattt tacccccacg aggtcattgc
     6541 tattcaaaat tattaaagca tttaaccgaa aataattttg atatcaataa aagagacagc
     6601 ggaattatcg aattattatt tactgctgag agagcacaag acataggtca tgcactggag
     6661 cgatgttatt cacaagcgga acttgaggac agcaaagcag ttttgttgcc aattgatatc
     6721 gcggacagaa acgtagagag agtactcaat catacgcatt cattaaaaaa aattgtgggg
     6781 ttgtgtttgt ctcaatggct ggttggactc attgaaaatc aggggctatc aaccttctgc
     6841 cagcctattt tgcataatac cacactcaaa ccctatggtt ttgagtgcct tctcagggga
     6901 aagcaagata ataatatact gccttcctct gatttatttg gagcagcaaa atcgagcgac
     6961 atgctttttt tgttagataa aaaagcacgt atatgccaca ttgaaaatat gagcaaaatt
     7021 gacatcgctg acaaaaaaat cttcattaat tttaacccaa cagccattta cgatcccctt
     7081 ttttgcttga caacaacgaa ccaatcctta aaaaaaacat ccattaaacc tgcacaaatt
     7141 gtatttgaag tcactgaaag tgatgaagta aaagataaaa aacacttgtt aaaaataata
     7201 gattattaca gaaatcaagg ttactgtgtc gcccttgacg atttgggctc tggttacagc
     7261 tctttgaatt tgcttactga gttacaacct gattacgtta aacttgatca agaattgatt
     7321 agccagattc atacaaatga attcaaacaa atcatattgg aaaaaatatg tgagatggct
     7381 actaaactaa agataaaaat aatctgcgaa ggcatagaaa cagaagacga gttacagatt
     7441 ataaaaaatt atgccatcga tttttatcaa ggttttttgt ttgagaaacc tatgccttac
     7501 gaaaaaatac ccgactatat caaagcaagc gcataaaaat ggattggcta ttggatatat
     7561 gacaaatgct tacaaacatt gaaagagcaa aacttcaatt aattgatgaa accaacgata
     7621 tggtcattat aaccaaagca gaaccacttg atgcacctaa tggtccggaa gtggtttggg
     7681 tgaataaagc ctttgaaaac atttcaaaat ataaagccaa tgaaattatt ggccaaacac
     7741 cacgtatcct tcaaggtaaa gaaaccaatc aaaacacatt aaaaatgata aaagaagcac
     7801 ttaaagctca aacgaatatc aatgtggatt tattaaacta tactaaggat ggaacacctt
     7861 attggattag tttttcaata gtttatttaa gagacgggca gggtaattta tgttatttag
     7921 gcgcaataga aaaagacatc accgcactca agaatttaaa ttttaaatta tctgaaaaag
     7981 caaagactga tccgcttacc aatgtttaca atcaggaaaa tttcttcatc gcagggaatg
     8041 aagctattaa aaactttcat caaaaccagg aagcagttgg attattattt ttcgatgtgg
     8101 atgattttaa aggaataaac gatacgcaag gacatatcta tggcgatatg atattaaaaa
     8161 aagttgccca ggaatcttta aagcttgtcc gtaaaacgga taacgtattt cgatatggcg
     8221 gcgatgagtt tgctattatt ttttcaggca ttgatgtgaa aatcctggaa agaaaagcac
     8281 aacaactaca aaaaaaattg gcaaaatctc acatctctgt gagcattgga gggactattt
     8341 caatccaaag tgacaaatcg ataaacaccc tcttggcgag agcggatgaa gcactctatt
     8401 acgtcaaagc caacaaaaaa gggttatttt tcatcgcaga ataatcacat tttgacgctg
     8461 ccaaattgcg aggaaattaa aaccagtgac gttcaatcca atgacatcgc cttttaaaca
     8521 attcctttat gccatgaaaa aataattatt ttcagtttta accatgcttc tctcaaaagt
     8581 cattttacat aatcatgtgt tacatcatat tgttccttaa tattattgag gtgagtctga
     8641 ctaaaagtct attgctcagg acttacgaaa aaccccaagg tcaaggcaaa aaatattgct
     8701 aataagggag tttagataaa ctaaatggct aaattaaaaa catttttaac aaagagtgtg
     8761 ggttttttcg taagtcatgt tgatgttgaa ttaagctggt tgggaataaa gctttttcaa
     8821 gaccacttca agagccttag cactggctga atcctggtct tcaagggcaa aatttgacgc
     8881 tttttccagt gtaattgtgg tcttgttaaa caaacaatgg cgtccagatc gtcctccaaa
     8941 aaagcctggt tgataattgg caaagaaatg cttatgaaaa gttgggggag acaggaggct
     9001 aaacaaatcc tccgtagtat aggcatcatg agtgagtctc ccaaccagga acccacccag
     9061 atagaacatc caagggctgt tatccagttt taatacggcg cgacctaatg agtcagttgg
     9121 aaaattccat tgcatttcca tgggttttcg gcctacgctg tttaggttcg cttcaatcaa
     9181 cgtgtattta aaaaaaggag agcgtgacag ttgctgggca aggccaccag agattcctgc
     9241 tcgacaagat tcaaggctca atatcttggg ggcttccttt aacccattag tagttaatcg
     9301 tctgattaga tcatctgctg tatccccctg tatatacaat ccggtatcct ctgctggtgt
     9361 tggcgcaagt gtggaatgtc ccatgattgt cagatgatct tcgcatgtga cttttcctaa
     9421 ttcctgatcg tttaataaga tataaccatg atccttggca ttggtttgct ggccaagcaa
     9481 agttgcctca gtagactcta aaacatagga tggatgaaac cagatgtatc gcttcataga
     9541 gaaaatctct tttattaaac aagatgcact taaacgatac aaatttattt gttttttgta
     9601 aatgcaaaat tcgatatttg ttgtcttttc aaatttaaaa taatgtcccc agctcaggaa
     9661 aataacttgt gaagccaagc ttacaagaaa ggcttgtccc tatttttata gtggtggcac
     9721 ccaaaacccg gggttggcag ccctccttcc cattgcatta ttctgacttt ttacaggtat
     9781 ttccaagttc ctggatgtca aaacgaaatg cctttatctt gatggctttt aacattcact
     9841 ttttcagtcg actggtcaaa aaagtttggc tgttgtccga ggtcagggtg acgatggcct
     9901 cttgggtcgg tatggcactt cataaacaaa cctaggcgct caagacgctg attcatgttc
     9961 tttaattcca ttttagagta gatcccttgt ctattttcaa cttgatttaa cgccgtattg
    10021 acagaacgca aatggccgta gttggtagtg tttttagtaa atcattgtag tgcacccgct
    10081 ccaaagactc ttccagcgat acaaacaagt cagcaaaaaa tgattttgca ctggtatatc
    10141 gacgttgtgg cagccttgcc acagatggtt tgtcaactac tgacgaccct tgcattagtt
    10201 taaagagcaa tactcctaat taagcgtttt tacataacag ttacataaca gagagtttaa
    10261 aaacaaacag ggatttacta gattgaaaag gtcacatagg ggtctgcctt caatcacgtg
    10321 tttacttatc ccgtctgagg agttcaaatc aagtacacca cgttataata gtctatccta
    10381 tatggtatag gagtatgtag gcaaatgtct ttaggcaatc tcattatcat attattggtt
    10441 aaaagggata cgaacttatg aaacccactc tatttttagt attaatgtgg atctgtgccg
    10501 agtcttttgg agcaactatc atggtaaaag gactgccatc tcctttaggg tacagcaatg
    10561 atctttatta ttggcctcca aatgtagcta tcagccctga cactaccaat ttattcatta
    10621 caatggatgg aattaacaag gtatgtttct tgaatacagg actacaaggg ggattagagc
    10681 aaatttccga gattagtatc atcatcaatg gagtaaaaaa agattggaat tgtttcccct
    10741 atacaacgac tattcttgag gctcgcccat aggaatacca cagcagcaaa cacgtctttc
    10801 caaatggttg aacacgcata aagatttcac caaataaaag gattttcctt gctcccattt
    10861 aagttaattt aagagccttt cacatctcga acaaggacct tgctctaacc cactcttctt
    10921 tggataagct cacattgtcc ctttataacc gtaaattgtc ctatttaaac cacaattaaa
    10981 ctggacattt aaagtacaat tattgtgtat tttattcatt tgaaatattt tacggactat
    11041 ttgagtttta cttacaccaa aacaactttt aacctgagga tagataatga aacctatgaa
    11101 aaacagtaaa gaacaagaac atattgctcc cgaagctcaa gaccccagat tgagctacac
    11161 cgaggacggc cgtcgcccat taacgagcac aacatcagcc aaacacccta atagtttgtt
    11221 cgctaaactg gaagaacgct cagcaaggga gagttacgat caatttggta acaatgttac
    11281 tatgcattgg accaaagacc cctggaatta gtgctctagc ttatgggttg attcgttgcg
    11341 aaccaaccca agattgcctc aaaattactc ctcctgttta atacattaat atcaaaacag
    11401 caggcactgg gatggtaatt ttctacgatt ttaattagca tcaaaagtag agctttaaga
    11461 tatataatga aataaattgc aagggtatca attgaatgtc aatttttaat aaattcaaaa
    11521 aattttacag atcctcatca gaaaacagga cacagattca cctttttctt ggctttgtta
    11581 taattccaat aataggcatg tctgttttat atatttgcgt aagaatattc tggatgtagg
    11641 aaaaaaactt tgatttttga ccagagtttt acaaccagtt aagatcc
//
LOCUS       DACTJE010000097        11627 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.097, whole genome shotgun sequence.
ACCESSION   DACTJE010000097 DACTJE010000000
VERSION     DACTJE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11627
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.097"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1058
                     /locus_tag="I9760_001968"
     CDS             <1..1058
                     /locus_tag="I9760_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAT5043731.1"
                     /translation="AIELAGGTLGSKSPIHPNDHVNMSQSSNDTFPTAMHIAAAIAFN
                     EKLLPAVRNLRHVFAAKMDAFKNIIKIGRTHLQDAVPLTLGQEFSGYVAQLDACVHRL
                     EEVLPELYELAAGGTAVGTGLNTHPQFAVKVAEHIAKITKLPFVTAPNKFAALASHEP
                     LVHAHGAMKTLACALMKIANDIRWLASGPRCGIGELIIPENEPGSSIMPGKVNPTQCE
                     AMTMVCAQVLGNDTAVGIADSQGNFELNVFKPVIIFNVLHSLNLLADSCHSFQEFCAE
                     GIEPNQPVIDYYLHHSLMLVTALNQHIGYDKAAKIAKTAHHDNISLQEAAVKLGILTA
                     EQFAEFVKPEKMISPEQ"
     gene            complement(1150..1773)
                     /locus_tag="I9760_001969"
     CDS             complement(1150..1773)
                     /locus_tag="I9760_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043732.1"
                     /translation="MTALIYSKLFYSICICYFILNRIKATSLRNALVFIVIICTCSNL
                     SYGNTNSITEILYSILGMPSIVSFIYCILLLLHQFRYVDWLLSGKTKFYIFFITIIFY
                     CSYLNILSPNVFYMGQLTTLLLCLAISLIAALINLYLAYLYLMSITIGVIFLAQSYNL
                     FVLVFDPLVFFLLLAQPRHIVKASILKDACKQNEIDLHIKNQTHLVE"
     gene            complement(1770..2942)
                     /locus_tag="I9760_001970"
     CDS             complement(1770..2942)
                     /locus_tag="I9760_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HAT5043733.1"
                     /translation="MKMNIFQMLMSSKNHLVYLGWLIIVFFIGLLTSKAPKNCAFELS
                     QPIKIENNTNANPVYIREKLPMPKGIKKAHSATLTKVDDSHLLAMYFAGSREGGPDVK
                     IYSSIYDIHRNKWSTPKIVLTREKLRKNSHQLIRKLGNPVIYNQNGKLHLFVVATAFA
                     GWAASKIYHFESLDKGKSFNYKATLYLSPFFNLSTLIRASPLPLVDGGFFLPAYHELE
                     SKYPLMLRFDKKGSYTESIQINHIGGQLQPSIIPLSSLNCLAGFRNANKTDHWMRIQY
                     CQEGGLSWLDPQKTNIKNMNNSINLFNFNSEVFLVHNRTHKNNIKGQLVLSRMENSTG
                     IFSYLFTLDTGEINNKSVTYPSTLVDRGYVDIIYDKNANEVIHIRFNKYYLDSLKK"
     gene            complement(2981..3817)
                     /locus_tag="I9760_001971"
     CDS             complement(2981..3817)
                     /locus_tag="I9760_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HAT5043734.1"
                     /translation="MTNHYLSKLRVTFAGLLFYWILPLRKQIVLSNIDLVFKNSATPA
                     EKTRLAKAFYSHVASNLKELIWMGWASSKGLTNKIEIHGLEHLESAVSQNKGVFLLTG
                     HLGNWELAYTLGFSRLMPQYGQFNIIRRPIKIKWLESILFNRMIRYGLTITSSISAPK
                     KINQALRNKELVLFTLDQHAKLENKSGIAVEFFGLHAGTYRSLAFFAHKHQTPVVPVS
                     GYRLHNGKHVIKFHPEIEWEFHVDKEQAIYRNTLRYNQMLEKLILEYPEQWWWVHRRW
                     KL"
     gene            complement(3810..4658)
                     /locus_tag="I9760_001972"
     CDS             complement(3810..4658)
                     /locus_tag="I9760_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HAT5043735.1"
                     /translation="MFELKRDLNSLPLTWLGRCVYFFSSYKRSNAKKNIDRVFQFSLS
                     PAEKKRLCMAYYSHMLTSIKEIILYSLLSKKTLEKRVKIIGIEHLENALSKGKGVLVL
                     TGHFGNWEFAPLFFLDKFDGRNFDFYCIRKNLRFAFLDSIFLRRFENCGFKIVSHKNA
                     VSQTRRVLKNKAIVFFPFDVCPSSKIKNKIKTDFLGRKTETNLSLAYLASLYDSCVLS
                     ITFYRTNKKQHVVHIYPEIKPVVYPDYKQTLVENTQLYNKRLEEMLLPYPEQWLWSYK
                     RWKNND"
     gene            4880..5950
                     /locus_tag="I9760_001973"
     CDS             4880..5950
                     /locus_tag="I9760_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043736.1"
                     /translation="MNKKSILLMSMLLVSPLSMSGTVGEAQQDGKRFVFSASAIYGSL
                     SDGTLDTREYADTIKSSGLISSGLFNVDSRWGYSLSAGYLFGPEFSYDLVLSYTNIKN
                     HGSSITVNSAQGDLMDNRLSLILKDFSGLQMIGPALAAVNSDYDYQTADLITHRTVPS
                     KFAKQLHYTRYYGAKAAEIVKEFTARYSGESRFGFTLPPAIQPLADNINFHAKYKGIG
                     PKAGAGVYWDVNRFITVGGDISFSVLAGNSKSHWNERVEITGAQPILDFPNTGGRYAY
                     FLNHPSSLWVSPVLGANLVVASHFNLNNDSVIGVEGGIGSEQYWSMNEGERYGLSNGG
                     NRINISERFAVRNVFIKASYIV"
     gene            6104..6934
                     /gene="lpxA"
                     /locus_tag="I9760_001974"
     CDS             6104..6934
                     /gene="lpxA"
                     /locus_tag="I9760_001974"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAT5043737.1"
                     /translation="MQMSQLDHVHAVNNLYMLSHQIHPTAIVSANARIGRDVVVGPYS
                     IVEDNVSIGQGTVIGSHVSIKSWTEIGEYNQIETGAIIGAIPQDLKFSGENSTAIIGN
                     NNIIREYVTISRGTSGGGGITRIGNNNVIMTSAHIAHDVQMGNHNIISNAVAVAGHVI
                     IDDWVTIGGLCGIHQFVQLGRMSMIGSQTRITKDVLPFTLVCGNPAKRFGINIERLQR
                     NGYSSVARMQIQRAYKILFHDGHLLTNAIEILKREFIDNNDVAYILKFLENSKRSFYR
                     "
     gene            6950..7981
                     /gene="lpxD"
                     /locus_tag="I9760_001975"
     CDS             6950..7981
                     /gene="lpxD"
                     /locus_tag="I9760_001975"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAT5043738.1"
                     /translation="MSNYQFTKPAGPFRLFELAKISGATLYEGKGETFTVSGLAKLSE
                     ATSNDLVMLHQKKYVKELKHTAARVCIIGPDYVKYAPDSMYLLVHPNPYKAFALIAQA
                     FYPSEKPPGFIATSAMIESSAIIGVDCFIAHGAYIGNQVKIGNRCKIGVNTYIGDTVT
                     IGDDCLIEDNVSIRHAVIGNNVVIYSGARIGQDGFGFASDANGHYKIPHAGGVIIGND
                     VEIGANTCIDRGSLDNTVIEDWCRLDNLVQIGHNVKIGKGSVLVAQVGIAGSTELGEH
                     VTLAGQVGVIGHLKIGKGATVLASAKVYKNVKSGDRVGGHPAVSISDWQKQIRFLKTA
                     IKSKKSPKS"
     gene            complement(7953..9104)
                     /gene="lpxB"
                     /locus_tag="I9760_001976"
     CDS             complement(7953..9104)
                     /gene="lpxB"
                     /locus_tag="I9760_001976"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAT5043739.1"
                     /translation="MKRPTRIAMVAGELSGDLLGAGVIRELKQHLTNVEFMGVGGPQM
                     LKEGFHSLIDISELSVMGISDVLRRYPQLYLIRERLLREWTINPPDVFIGIDYPDFNL
                     SVEARLKKQHIKTIHLVSPKVWAWRQKRVHLIKKAVDLVLTLFPFEEAFYRQHGVSAQ
                     FIGHPLADLIEINPSCSTLRKKYNYHSDDTILAVLPGSRVGEIKYMGPLFLEVMQRIA
                     VERPHVHFIVPIACQDLYPVFFKQLHAEYGHLKIQIIQGNAREAMAISDVVLTKSGTA
                     TLEAMLLKRPMVVAFKWGILTHAIIAPQVKVPYIALPNLLAGKKLIPEFVQEKANVDS
                     ITESVLNLLDSSNQNELIKQFTDIHCTLRQNANEKAALSILRILGTSLT"
     gene            complement(9288..10145)
                     /locus_tag="I9760_001977"
     CDS             complement(9288..10145)
                     /locus_tag="I9760_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043740.1"
                     /translation="MFYYRAYQLNIQSDIPLGKIEQTSYCVPDVIIRKTSEIPDIPTN
                     ASRLGNKYASLKNQLWITDNQWSFYIEEGSTILYQIKSVELTTPIHILLTGACFGCLL
                     QQRNLLVLHANTVCYPGQPAFAISGHTGAGKSTAAAFHIKQGALLAADDITAIEFKQD
                     IPHVLPGFPRLKLWNDSREALHLLSLESLYSYNGRSKHHIDLKPNQHCSTPIVLEKLV
                     IIKPDLAEERELFGKDKIQCLLANNYTALIFHLFDFHWDVFKNNLKLVRQLKIYNAPR
                     PAMVFYNEI"
     gene            complement(10175..>11627)
                     /locus_tag="I9760_001978"
     CDS             complement(10175..>11627)
                     /locus_tag="I9760_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5043741.1"
                     /translation="MKNFERYCRESLIQNLVNARWEYLLKSKLKDSEYVLNVGLMKIS
                     ALTTYTLSLFNNIIKNSVYLIVGLMLSAGLTVISLITVISIAIFFKKSNSAGMGKQEL
                     DLSRSRQINLSNFLEGIKIAKIHELTHEYLKQERDISFKVMDNNIKFLSNQMKIKFGY
                     TAIGAIAFTLIFLISISLLQISIPTLVVLLFVYNQIIRRISTMQQSMSYALNISPLFR
                     NYLILKNDFLSNKESSNVIPFHTRVPLPKDILIKVKQVSFSYGEKAILKNVSFDIHRN
                     KITAIKGPSGVGKSTCVDLILGLLKPDDGSILFNQSVVPMDVEHATSKKKWVSYVPQN
                     IFLFNDSIRNNITWLNNDVNEEKLWKVLALVDAENLVRSMPNGLNTMIGDRGVHLSGG
                     ERQKLAIARAILSSPTLLILDEATSALDPQSEAVIMQSLAALKQTMTVILIAHRESTI
                     VYADEIIDFGKFNLKEEYPLNFNVLEDTTVAMN"
BASE COUNT         3622 a         2226 c         2241 g         3538 t
ORIGIN      
        1 gggccattga gctggctggc ggcaccctgg gaagcaagtc ccctattcat cccaatgatc
       61 acgtcaatat gtctcaatca tcgaatgaca ccttcccaac agccatgcat attgcagcgg
      121 cgatagcttt taacgaaaaa ttgttacctg ctgtacgcaa tttacgtcat gttttcgcag
      181 cgaaaatgga cgctttcaaa aacatcataa aaattggcag aacccatttg caagatgcgg
      241 ttccactgac tttggggcaa gagttttcag gttatgttgc ccaactggat gcgtgcgttc
      301 accgtctgga agaagtcttg cctgaattat atgagctggc tgccgggggc actgccgttg
      361 gaaccggatt gaacacccat ccgcaattcg cagtcaaagt tgctgaacac atcgctaaaa
      421 taaccaaact gccttttgtg accgctccta acaaatttgc cgctctggct tctcacgaac
      481 ctctggtgca tgcgcacggt gccatgaaaa ctctcgcctg cgcattaatg aaaatcgcca
      541 atgacatacg ctggctcgcc tcaggacctc gttgcgggat tggtgagctg attattccag
      601 aaaatgagcc aggctcatcc atcatgccag gcaaagtcaa cccgacacaa tgcgaagcca
      661 tgaccatggt ttgtgcccag gtactgggta atgatacagc cgttggaatt gctgacagcc
      721 agggtaattt tgaattaaac gtgtttaaac cggttatcat atttaacgta ttgcactcat
      781 taaacctgct cgccgacagc tgccattcct tccaggagtt ttgcgcagaa ggaatagagc
      841 ctaatcagcc ggtgattgat tactatttac accattccct catgctggtt actgccctta
      901 atcaacacat tggttatgat aaagcggcta aaatcgccaa aaccgctcat catgacaata
      961 tttcattgca agaagccgcc gtgaaactgg gaattttaac cgccgagcaa tttgctgagt
     1021 ttgtgaaacc ggaaaagatg atttcccctg agcaatgaaa ttgagagggt taagacatac
     1081 cggggttctg gtatgtctta acctaagatg tagtagaaaa caaaatgaca acaaacaata
     1141 gtttgaattt tactcaacca aatgggtctg attttttata tgaagatcga tttcgttttg
     1201 tttacaagca tcctttaaaa tagaagcttt tactatgtgt cttggttgag ctaataacag
     1261 gaaaaatact aatgggtcaa aaactaaaac aaataaatta taggactggg ctaaaaaaat
     1321 tacacctata gtaatgctca tgaggtataa ataggccaaa tataaattta taagcgctgc
     1381 aattaaagaa attgccaaac agagcaatag tgtagtcaat tgccccatat agaacacatt
     1441 aggagaaaga atgtttaaat aactgcagta aaaaataatg gtaatgaaaa aaatatagaa
     1501 tttagttttc cctgaaagta accaatcaac ataacgaaat tgatgtaaga gtagcaaaat
     1561 acaatagata aaacttacaa tgctaggcat tcccaaaatg ctatatagta tttcggtaat
     1621 tgagttggta ttaccataac tcaaattaga acatgtgcat atgataacta taaaaactag
     1681 agcgtttctt aatgaagttg cttttatcct gttaaggata aaatagcata tacagattga
     1741 ataaaacaat ttgctataaa ttaatgcagt cattttttca aactatccag gtaatattta
     1801 ttaaaacgta tatgaatgac ctcattcgca tttttgtcat agataatatc aacataacct
     1861 ctatccacta atgtagaagg gtaagtaaca cttttattgt taatttcacc cgtatccaaa
     1921 gtaaacagat acgaaaatat tccggtcgaa ttctccattc tggataacac aagttggcct
     1981 ttgatattat ttttatgcgt tctgttatgg actaaaaata cctctgaatt aaaattaaat
     2041 aaattaatag aattattcat atttttaata ttcgtttttt ggggatcaag ccatgaaagt
     2101 cctccttcct ggcaatattg gatccgcatc cagtgatctg ttttattagc attacgaaat
     2161 ccagctaagc aattcaagct tgaaagggga atgattgatg gctgtaattg ccccccaatg
     2221 tgattgattt gaatgctttc tgtatagctc ccttttttat caaaccggag cattaaagga
     2281 tatttgcttt ctaattcatg gtatgctggc aaaaaaaaac caccatctac taatggaagg
     2341 ggggatgctc tgattaaggt acttaaatta aaaaagggac ttaaatacaa ggtagcttta
     2401 tagttgaaac tcttcccttt atctaatgac tcaaaatgat aaattttgct agccgcccaa
     2461 cctgcaaaag cagttgcaac aacaaacaaa tgcaacttgc cattttgatt ataaataaca
     2521 ggattaccta attttctaat caattgatgg ctattcttgc gcagtttctc tcgagtcagc
     2581 actattttgg gggttgacca tttatttcta tggatatcat aaatagagct ataaatttta
     2641 acatcaggtc ctccctcacg actcccagca aaatacatgg cgagcaaatg actgtcatca
     2701 actttagtca atgtagcaga atgtgccttt ttaatacctt ttggcatagg taatttttct
     2761 cttatataaa caggattggc atttgtgtta ttttctatct taataggttg agataattca
     2821 aatgcgcaat ttttaggggc cttagaagtt agcaagccaa taaaaaaaac tattattaac
     2881 catcctaaat aaaccagatg attttttgat gacatcaaca tttgaaaaat attcattttc
     2941 attttgtaaa ataatatttg ggtttcttta catttttagc ttataatttc catcgtctat
     3001 gaacccacca ccattgctcc ggatattcca aaattaattt ttcaagcatt tgattatagc
     3061 gaagagtatt tctgtagata gcttgttctt tatcgacatg aaattcccat tctatttctg
     3121 gatgaaattt aataacgtgc ttgccgttgt gtagcctata gccggatacg gggactactg
     3181 gtgtttgatg tttatgggca aaaaaggcca aacttcggta tgtcccagca tgaaggccaa
     3241 aaaactcgac agcgattcct gatttatttt ccaatttcgc atgctgatcg agtgtaaaaa
     3301 gtactaattc cttgtttctt aaggcttgat ttattttttt tggcgcacta atgctgctgg
     3361 taattgttaa tccatagcgt atcatcctat taaacaatat tgattccagc cattttattt
     3421 taatgggtcg tctgatgata ttaaattgcc catattgagg catcaggcga gaaaaaccta
     3481 aagtataagc taattcccaa ttacctaaat gaccagtgag cagaaaaacc cctttattct
     3541 gactcacagc gctttccaaa tgctctaaac catgaatctc tatcttatta gtcaatcctt
     3601 tagaacttgc ccaacccatc caaatgagtt cttttaaatt agatgcgaca tgagagtaaa
     3661 acgctttggc taatcttgtt ttttcagcag gagtcgctga atttttaaaa acgaggtcga
     3721 tattactcaa tacaatttgc ttacgaagag gcaaaatcca gtaaaaaaga agccctgcaa
     3781 aagtcactcg gagtttggac aaatagtggt tagtcattat ttttccaacg tttgtatgac
     3841 cataaccatt gctcagggta aggcaataac atctcttcca atcttttatt ataaagctga
     3901 gtgttttcga caagagtttg tttataatcc ggataaacaa ccggttttat ttccggataa
     3961 atatgtacta catgttgttt tttattggta cgataaaaag tgattgataa cacacatgaa
     4021 tcataaagac ttgctaaata ggccaaactg agattagtct ccgttttccg tcctaaaaaa
     4081 tcggttttta tcttattttt aattttcgaa gaagggcaaa catcgaaagg gaaaaagaca
     4141 atggccttgt ttttcaaaac cctgcgagtc tgactcaccg catttttatg actgactatt
     4201 ttaaatccgc aattttcaaa tcgccgcaaa aaaatggaat ccaaaaaagc aaaacgcaaa
     4261 tttttgcgga tacaataaaa atcaaaattt ctgccatcaa atttatcaag aaaaaataac
     4321 ggtgcaaact cccagttacc aaagtgtccc gtcaatacta aaaccccttt tcccttgctt
     4381 agagcgttct ctaaatgctc aataccaatg attttgacac gcttttccaa agttttttta
     4441 ctcagcagcg agtataaaat aatttctttg atgcttgtta gcatgtggga ataataggcc
     4501 atgcataaac gttttttttc ggctggtgat agggagaatt gaaaaacgcg atcgatattt
     4561 ttttttgcat tacttctttt gtaagaagag aaaaaataga cgcagcgtcc caaccaagtc
     4621 aagggaagag aatttaaatc acgtttcagc tcgaacataa aaagaaaact accctgtgtt
     4681 ttgcctaagc actattcctg actatgaagc ctggtttaga caggcagtct acaatctaat
     4741 cattttaaag taaacagaac ttactgatac tgactaactc ctgcgaatac gttatttgta
     4801 gctggatatc attgaagttg tcctttataa tccagagcga acaaatacaa tattcaaaga
     4861 tcagggagta gatacataga tgaataaaaa aagcatttta ttgatgagca tgctcttggt
     4921 aagtccactt agcatgagtg gcacagtagg agaggcgcaa caagatggta agagatttgt
     4981 ttttagtgct tcagcaattt atggctcgtt aagtgacggc accttggata ccagggaata
     5041 tgctgatacc ataaagtctt ccgggctaat tagctctggc ttgtttaatg tggattcccg
     5101 ttgggggtat tcattaagtg ccggatatct ttttgggccc gaattcagct atgatttggt
     5161 attaagctac accaatataa aaaatcacgg ctccagcata actgtcaact ctgcacaggg
     5221 tgatctcatg gacaacaggc taagcctgat tcttaaagat ttttcaggac tgcaaatgat
     5281 tggccctgcc cttgctgcgg taaattctga ttatgattat caaaccgcgg atttaattac
     5341 tcataggact gttccaagca aatttgccaa acagctccat tatactcgct attacggggc
     5401 aaaagccgca gaaatagtaa aagaatttac cgctcgttat agtggtgaat ctcgttttgg
     5461 cttcaccctt ccccctgcaa tacagcccct tgcggacaat attaattttc atgcgaaata
     5521 taaagggatt ggccctaaag cgggagcagg agtctattgg gatgttaatc gatttattac
     5581 tgtaggcggg gatatttctt tctctgtatt ggctggtaat agcaaaagtc attggaacga
     5641 aagggttgaa ataaccgggg ctcaaccaat tttggatttc cccaatacgg gcggccgtta
     5701 cgcttatttc cttaatcatc cttcctcgct atgggtttct ccagttctag gagccaattt
     5761 ggtggtggcc tctcatttca atttgaataa tgacagtgta attggtgttg agggcggaat
     5821 cggctctgag caatactggt cgatgaatga aggggaaagg tatggtctgt caaatggtgg
     5881 taacaggata aatattagcg agcgttttgc ggtacgtaat gtatttataa aagcaagtta
     5941 tattgtttag ttcagacata acctgacacg acttgcgatg agcgcaagtc gtgtgactga
     6001 gtgctttccc tctatatcaa atcattttcc cagaacagat ttaagagtag tatagagcac
     6061 gttgtaacca gcatttaatt tatagttgta gtggaggtga accatgcaaa tgagtcagtt
     6121 ggatcatgtt catgcggtta acaatttata tatgctctcc catcaaattc atcctacagc
     6181 tatcgtatca gccaacgcca gaattggacg agatgtggtt gtaggtccat attcaattgt
     6241 tgaagacaat gtcagcattg gacaaggaac cgtcattggt tctcatgtgt cgataaagtc
     6301 ctggacagaa ataggtgaat ataatcagat tgaaacggga gcaatcattg gagcaatccc
     6361 gcaggatctg aagttcagcg gagaaaacag caccgccata atcggtaata ataacattat
     6421 tcgcgaatac gttacgatca gtagaggaac atctggggga ggaggaatta ctcgaatagg
     6481 aaacaacaat gtcataatga cttccgccca tatagcccat gatgttcaga tgggtaatca
     6541 taatatcatc tccaatgcgg tagccgtagc cgggcatgta atcattgacg attgggtaac
     6601 cataggcggt ttatgcggca tacaccaatt cgtacaatta ggacggatga gtatgattgg
     6661 ttctcaaacc agaattacaa aagacgtact gcctttcacg ttggtttgcg gaaacccagc
     6721 caagcgtttt ggtattaaca ttgaaagatt gcaacgaaat ggatacagtt ctgtcgcaag
     6781 aatgcaaatt caacgagcct acaagatact ttttcatgat ggtcatcttt tgacaaatgc
     6841 cattgaaata ctcaagcgtg aattcataga caataatgat gtggcttata ttctcaagtt
     6901 tcttgaaaat tccaaacgca gcttttatcg ctaatttttt agagtgtgta tgtcaaatta
     6961 ccaatttaca aaacctgcag gaccatttcg tttattcgaa ttagctaaga tttctggtgc
     7021 tacactgtat gaaggtaaag gagaaacctt tacagtttct ggtttggcaa aactttcaga
     7081 agccacaagc aatgatttgg taatgctgca tcaaaaaaaa tacgtcaaag aattaaagca
     7141 tacagctgct cgtgtttgta tcatcgggcc agactatgtg aaatatgccc ctgatagcat
     7201 gtatttactg gtgcacccca atccctacaa agcatttgct ttgattgctc aagcgtttta
     7261 tccttctgaa aaaccacccg gtttcattgc cacttcggca atgattgagt cgagcgctat
     7321 tattggcgtg gattgtttta ttgctcatgg ggcttatatt ggcaatcagg ttaaaattgg
     7381 caacagatgc aaaattggag tgaatactta tattggcgat actgtaacca taggcgatga
     7441 ttgtcttata gaagacaacg tcagcattag gcatgctgtt atagggaata acgtggtcat
     7501 ttattccggt gctcgtatag gccaggatgg ttttggcttt gccagtgatg cgaatggcca
     7561 ttataaaatt ccccatgcgg gtggtgtcat tattggcaat gatgttgaaa tcggggctaa
     7621 tacctgcatt gaccgaggct ctctggataa tacggtgatt gaagattggt gtcgtctgga
     7681 taatcttgtg cagattgggc ataatgtcaa gattggtaaa ggctctgtcc tagtggctca
     7741 agttggaatc gccggcagta cagagcttgg tgaacatgtc actttggcag gacaagttgg
     7801 cgtgattgga cacttgaaga tcggtaaagg cgccactgtt ttagccagcg ctaaagtcta
     7861 taaaaatgtg aaatccggag acagggtcgg cggccaccca gcggtatcaa ttagtgattg
     7921 gcaaaaacaa atccgatttt taaaaacagc gattaagtca aagaagtccc caaaatcctg
     7981 aggatagata aagccgcttt ctcattagca ttttgcctta atgtgcagtg aatatcagta
     8041 aattgtttaa ttagttcgtt ttgattggat gagtcgagta aattcagaac tgattctgta
     8101 atgctatcaa cattcgcttt ttcctgaaca aattcaggga ttaatttttt accagccaat
     8161 aaattaggca acgcaatata gggaactttt acttgcggcg cgataatcgc atgagtaagt
     8221 ataccccatt taaaggcaac caccatgggt cgttttaaaa gcatcgcctc aagtgttgct
     8281 gttccggatt ttgttaaaac aacgtcagag atagccatgg cctcacgagc attcccttga
     8341 ataatctgaa tttttaagtg gccatactcg gcatggagtt gtttaaaaaa aactggatac
     8401 aaatcctgac acgcaatcgg tactatgaaa tgcacatgag gcctctccac agcaattctt
     8461 tgcattactt ccaaaaacaa gggccccatg tatttgattt cacctacccg actacccggt
     8521 aatactgcca gaatggtgtc atcagaatgg tagttatatt ttttacgcaa agtagaacaa
     8581 cttgggttta tttcgattaa atcagctaaa ggatgaccaa taaattgtgc cgatacccca
     8641 tgttgtcgat aaaatgcttc ttcaaatgga aatagggtta atactaaatc aactgccttt
     8701 tttattaaat gcacccgttt ttgtcgccat gcccaaactt tggggctcac cagatgaatg
     8761 gttttaatat gctgtttttt taaccgtgct tcaacggata aattaaagtc agggtaatca
     8821 atcccaatga atacatctgg tggattaatt gtccattccc tcaataacct ttctcttatt
     8881 aaatacagtt gaggatatcg tcgcagaaca tcactgattc ccatcacaga aagctcggag
     8941 atatcaatca gggaatggaa gccttccttg agcatttgag gaccaccgac ccccatgaat
     9001 tctacgttgg ttaaatgctg tttcagctca cgaattaccc ctgctcccag taaatctcca
     9061 gatagttcgc cggcaaccat cgcaattcgg gtcggtcttt tcatagtcaa attgcaccaa
     9121 tataaaattt aaatgacttg atgttagcct aaactaataa ccatagccaa gcagtattaa
     9181 ttccgcaaac gcacagattg ccaattgagt gtcagaaaac gctttacaaa taatttttca
     9241 gcctggcaaa aatgtctgcc tgggcaagtc ctcttggcaa agatcatcta gatctcattg
     9301 taaaatacca tagccgggcg aggggcattg tatattttaa gttgtctgac taattttaaa
     9361 ttattcttga atacgtccca atgaaaatca aatagatgaa atatcagagc agtgtagtta
     9421 tttgcaagga gacattgaat cttatcttta ccgaagagtt ctctttcctc tgccaaatca
     9481 ggttttataa taaccagttt ttcaagcact attggtgtgc tgcaatgttg attaggcttt
     9541 aaatcaatat gatgcttgga tcttccatta taagaataga gtgactccaa agataataaa
     9601 tgtagggctt ctctactatc gttccataat ttcaaacgag gaaatcccgg caatacatga
     9661 gggatatcct gtttgaactc tatggctgtt atatcatctg cggccaataa agcgccttgt
     9721 tttatatgaa atgcagctgc cgtagatttt cctgccccgg tatggccaga gatggcaaaa
     9781 gcaggttgac ctgggtagca aactgtattg gcatgcaaaa caagcaaatt tctctgctgg
     9841 agcaggcagc caaaacaggc accagtaaga agaatatgaa ttggtgtagt cagctcgacg
     9901 gatttaatct ggtataaaat tgtacttccc tcttcaatat aaaagctcca ttggttatcg
     9961 gtaatccata gctgattttt taaagacgcg tatttgtttc ccaagcgact cgcgtttgta
    10021 ggtatatcag gaatttcact ggttttacga ataatgacat ctggaacaca ataagaagtt
    10081 tgctctattt ttccaagtgg aatgtcactt tgtatgttca attggtatgc gcgatagtaa
    10141 aacataaacg tctttataaa aaattgataa ataattaatt catagcaacc gtagtgtctt
    10201 caagaacatt gaaatttaac ggatactcct cttttaaatt aaacttgcca aaatcgatta
    10261 tttcatccgc ataaactatg gtactttccc ggtgagctat taaaatgact gtcatggtct
    10321 gttttaaagc agcaagcgac tgcataataa cagcctcgct ttggggatca agtgcactgg
    10381 ttgcctcatc gagaatgagc agcgttggcg atgacagtat ggctctggca atcgccagct
    10441 tttgccgctc tccgccagat aaatgcactc ctctgtcacc aatcatagta ttcaatccat
    10501 tgggcataga cctgaccagg ttttcagcat caaccagggc aagaactttc catagcttct
    10561 cttcattcac atcattgttt agccacgtga tgttattcct gattgaatca ttaaaaagaa
    10621 aaatattttg aggaacataa ctaacccatt ttttcttgga agttgcatgt tccacatcca
    10681 ttggaactac ggactggtta aagagaattg agccatcatc cggcttcaac aagcccaata
    10741 ttaaatcgac acaggtggat ttcccaacac cagatgggcc ttttattgca gttattttat
    10801 ttcgatgtat gtcaaatgaa acatttttta aaattgcttt ttctccatag gagaaggaaa
    10861 cttgctttac tttaattaat atatccttag gtaatggtac tcgcgtatga aagggtatca
    10921 catttgatga ttctttattt gataaaaaat catttttaag gattaaatag tttcgaaaca
    10981 agggagaaat attcaatgca taagacatgc tttgctgcat cgttgatatt cttcttatga
    11041 tttgattgta aacaaataat aaaacaacca acgttggaat tgaaatctgt aacagggaaa
    11101 tggaaataag aaaaattaaa gtgaacgcga tcgcgccgat tgcagtatat ccaaatttaa
    11161 ttttcatctg gttgcttaaa aatttaatgt tattgtccat caccttgaat gagatatcac
    11221 gctcttgctt gagatattca tgtgtaagct catgaatttt agctattttt attccttcaa
    11281 gaaaatttga caaattgatt tgcctgcttc tgctcagatc aagttcttgt tttcccatac
    11341 cagcagaatt tgatttttta aaaaaaattg ctatcgaaat cacagtaatt agcgaaatga
    11401 ctgttaatcc cgcagaaagc attaaaccaa caatcaaata caccgagttt ttgattatgt
    11461 tattaaataa actcaacgta taagtcgtta aagcagagat cttcatcaac ccaacattca
    11521 atacatactc agaatctttc agtttacttt ttagcaggta ttcccatcgc gcattcacca
    11581 agttttggat taagctttca cggcagtatc tctcaaaatt tttcata
//
LOCUS       DACTJE010000098        11623 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.098, whole genome shotgun sequence.
ACCESSION   DACTJE010000098 DACTJE010000000
VERSION     DACTJE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.098"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            131..1093
                     /locus_tag="I9760_001979"
     CDS             131..1093
                     /locus_tag="I9760_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HAT5043742.1"
                     /translation="MNFCTLGLAVIETEAQAVFELTQRIDEHFEKACELLLSCKGRIV
                     VTGMGKSGHIANKLASTFSSTGSPAFFMHPGEASHGDLGMITRQDIVVAISNSGNTHE
                     IVTLLPLLKRLEVPLITLTGNKQSTLAKSADVNLDVSIKQEACPLGLAPTTSTTVSLV
                     MGDALAISLLQARGFSAEDFALSHPGGALGKKLLLKIDDLCHTGEQLPLANENATVSD
                     ALIEVTNKKLGMTCVVDNHGYLVGVYTDGDIRRTLTRQFNINTTLIKDVMTKNCRTIY
                     KGMLAAEALAIMQKYSITSLVVVENDNRPYAVLHLHDLLKAGVF"
     gene            1107..1634
                     /gene="kdsC"
                     /locus_tag="I9760_001980"
     CDS             1107..1634
                     /gene="kdsC"
                     /locus_tag="I9760_001980"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAT5043743.1"
                     /translation="MNELLEKAKKIKCLICDVDGVLSDGLLHIDNHGNELKSFHVQDG
                     MGLKLLMAAGIQVAIITTAQNAVVDHRMEQLGITHYYKGQVDKRSAYQHLKKALGLND
                     EEFAYIGDDLPDLPLIQQVGLGVAVSNAVPQVLEFADWRTERTGGRGAVRELCDLILN
                     AQNKAELAITGYLKQ"
     gene            1631..2200
                     /gene="lptC"
                     /locus_tag="I9760_001981"
     CDS             1631..2200
                     /gene="lptC"
                     /locus_tag="I9760_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAT5043744.1"
                     /translation="MNITKQAIWLFLTLLILAGSGWYYAHFNQAIINKKTLSNEVDTT
                     ISQLTVKQFNSEGVLINLLTTPQVEYIPNNNLHLLKNPHIIIKQDNQPAWEINSLRAK
                     SFDGGKQITFIEQVVVHQNPGESTRESTLKTEEITYYPKEKKATTSLFVTFEQPGNII
                     QSTGMNAYLDEKRVELLHKARGSYDPAKG"
     gene            2181..2690
                     /gene="lptA"
                     /locus_tag="I9760_001982"
     CDS             2181..2690
                     /gene="lptA"
                     /locus_tag="I9760_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HAT5043745.1"
                     /translation="MTQLKVKYIILLLLMLCSISGNALPFDKEKMVQVVSDSADLNQK
                     NHQGVYIGNVEFVQGSTNLRAEKAITQGDAKNQLTLAIAEGSKDKQAHYWTTTAPDKP
                     PFHAYADTIKYYPLKHLIELIGNARIEQGPNSLSAAKISYDTQEQHVLSHSDEKTRTT
                     IIIYPEKKS"
     gene            2687..3412
                     /gene="lptB"
                     /locus_tag="I9760_001983"
     CDS             2687..3412
                     /gene="lptB"
                     /locus_tag="I9760_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAT5043746.1"
                     /translation="MSLLEAKQLKKSFKGRTVVNGVSIHIKQGECVGLLGPNGAGKTT
                     CFYMIVGLQSCDEGHIILNGADITQDAMHLRARHGISYLPQEASVFRKLTVAENIMAI
                     LELRKDLDDQKREEKLDLLLQEFHISHIENSLGMALSGGERRRVEIARALAIEPSFIL
                     LDEPFAGVDPISVIDIKKIILHLCDKGIGVLITDHNVRETLDICERAYIVSQGKILCE
                     GTPKAILANQQVRQVYLGEEFAL"
     gene            3446..4024
                     /locus_tag="I9760_001984"
     CDS             3446..4024
                     /locus_tag="I9760_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HAT5043747.1"
                     /translation="MLLIIDNYDSFTYNLVQYFQSLNQKVEVFKNDEIQIDTIRQSAP
                     QYLVISPGPNSPDESGISIEIIKEFHKHIPMLGICLGHQCIAQAFGGKIIPAPEIMHG
                     KTSEITHHYQGLFTGIPNHFQATRYHSLAVDIMSLPNCFSIDAWADDTIMAISHRQFP
                     LFGLQFHPEAILSQFGLQILENFLRYETQTSI"
     gene            4002..5036
                     /gene="trpD"
                     /locus_tag="I9760_001985"
     CDS             4002..5036
                     /gene="trpD"
                     /locus_tag="I9760_001985"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HAT5043748.1"
                     /translation="MKPKLLFEQLLSRQDLSSDQMQEVIHACMTGEFSDVQIATFLAL
                     MRMKSETVNELTAAAKVMRQLAHKIDLGNPLIDIVGTGGDGRNTFNVSTACSFVVAAA
                     GIKVAKHGNRSVSSRSGSADLLEQAGFILNLSDSQVQNCINQCQLAFLFAPHYHPAMQ
                     HARAARQQLGIRTLFNLLGPLINPAQVKRQVVGVFSTNWLKTIATVLANLGSERSLVI
                     SSQDGLDEISIAAKSEVVEYRDGNFKQWFISPEDYGLKHSSLDAIIVDSPEQSLHLIQ
                     SVLSGDSGPARDIVLLNSAAAIYCAKDGISFDASIEEARIAIDSGKANHCFNKLRLLT
                     QTLNKESNHE"
     gene            5029..5805
                     /gene="trpC"
                     /locus_tag="I9760_001986"
     CDS             5029..5805
                     /gene="trpC"
                     /locus_tag="I9760_001986"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HAT5043749.1"
                     /translation="MNSILERIAKHKLEEVAVAKKNKPLHVLSKQQPGEIRDFITALK
                     SNTSPAVIAEIKKASPSKGLIRKDFNVAEIAKIYTQNGARCLSVLTDIEFFQGHPDYL
                     ALAKSKTTLPVLRKDFIIDSYQIYESLVLGADCILLIVALLDDVQLMDFCQLAQELKM
                     SVLVESHTQDELERALRLPTPLIGINNRSLHNFKTDIQLSIQLKQFVPKDKIIITESG
                     INTREDIKLMQSHGINAFLIGESLMRADNIGKALQKLMTD"
     gene            complement(5811..5978)
                     /locus_tag="I9760_001987"
     CDS             complement(5811..5978)
                     /locus_tag="I9760_001987"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043750.1"
                     /translation="MNTTEIPEQPIEYPEEPSEYPEQPLEYPDNPEPLEPGYPELPEP
                     EPSQTPEPGFE"
     gene            6281..7129
                     /locus_tag="I9760_001988"
     CDS             6281..7129
                     /locus_tag="I9760_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAT5043751.1"
                     /translation="MNVVIITGAASGIGHAVSQVCLGSGKTIVMVDKNQDKLHTEAQS
                     FMTKFSPEHVICFACDVTQPSEVERLAEYSCKQLGQVDWIFNNAGIIGNLLPAWELQE
                     NDINQVMQVNLHGMLNIIRSFMPYLFKQNFRSHIINMASLYALCTGSQMAAYSMSKHA
                     VLALSESLYFDLNRLKKPVDVSVVFPSFTDTSLLASSGKLNHSPIHNQLNNLLAHSRP
                     AIEVAEHIVREVEQKRFYILPDKEVKGYCEDRTKAILLQENPHRNSVEQLMCSLLKRQ
                     SSEANL"
     gene            complement(7222..8562)
                     /locus_tag="I9760_001989"
     CDS             complement(7222..8562)
                     /locus_tag="I9760_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HAT5043752.1"
                     /translation="MEIKRVLKDRLSARVCVIGAGPSGIAAIKNLQEHGITNVTVFEK
                     NNQIGGNWVYDEQNEHSSVYETTHIISSKRWSEFEDFPMPVDYPDYPSHSQLLKYFQS
                     YVEHFHLDRYIRFNTVVQKVHRLDDNTWHVIYEDAQGIHEACYDYLLVANGHHWDPFM
                     PVYPGVFDGQILHSHQYKKASIFKGKRVLVVGGGNSACDVAVEISRVAPGTCISMRRG
                     YHIFPKFIFGKPTDVAVAKIQWMPSWLRQKFISLVIRGLQGRYAKYKLMKPDCGPLEI
                     HPTINSELLYFIRHGKIHPRPGISRFEGNTVHFTDGTQGEFDTIIFATGYQISFPFFD
                     KSCIDFSNSTTIPLYRKMMHPEFENLYFIGLCQPQGCIWPLADYQSKIVARIIAGTLK
                     RPDQLHRKIAKEINKPHHRFKSHMRHALEVDYHIFRKELLEMLGQKHKRLKFYP"
     gene            complement(8540..9265)
                     /locus_tag="I9760_001990"
     CDS             complement(8540..9265)
                     /locus_tag="I9760_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043753.1"
                     /translation="MNYFGKLQIRFSKSFLLLLIVSSLTACVDLTGARNTYIKKEKQP
                     AISDVPNKKSKAQNMSSKKGEVHTMLGGLGIFSKGMYTLRDKVSEKYHITAHSTMWYN
                     AGAVSRYIIDHYYQEKDHKPIILVGHSLGANEQIKVARNLNAAGVPVALLVTVDAVSQ
                     TIVPPNVKQALNVYKPGFVPMFSGLKLRAVDPQLTKIDNINVDSIKGIKVNHFTIDKD
                     EILQAMIMDKVDKVLVDGNKKGA"
     gene            complement(9401..11320)
                     /locus_tag="I9760_001991"
     CDS             complement(9401..11320)
                     /locus_tag="I9760_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5043754.1"
                     /translation="MTLTKKMAVGVLIMLLFVFIGTYFITMNNARNFFIQQLESNAQD
                     TATSLGLSLSQSLINHDVPTMDSMVKAVFDRGYFSSIKVQDIKGKVIILKKQLPQESD
                     IPQWFVNLIKWPSTEKSSLIMDGWMQAGVVLVASDPSYVYASLWRNAVEMVNAYLIFA
                     LVALVLSYGFLKYLLQPLKRVTAQALAISEHEFPVETKIPKTPELRQVTLAMNQMVTK
                     VKSLFQDQLKQTESLRTQVYQDSLTGMSNRRYFLQHLALILDNEDEFIPGYVMMLVID
                     GLDELNQKQGYQQGDQLVLTVAKICKSYWKQSSVSTLARINGTTFALISHERDPLVFE
                     KECQEFEQILSQGINDIKICKIHMGAASYFLHQPVSNLLSTVDQAVKKARETGVFYCQ
                     KEHDTYKYPQLISGDEIRNSLQQKKISLYAQAVTDGKNCFHKEVFVRIRNQEGEELGA
                     GYFIPVAEKLGLAYPIDQYVLNELTVMDIATHTHFALNISEDTLANKVNSTGYLRQLE
                     DTPAAVLRNLSLEINEAHVLSHFSNSKFFIKQAKKLGVTVGIDRVGIKFSPLHYLSDL
                     NIDYLKLHGSLVTDIDENESKQFFIHYFNEMAKTMDIAVVATQVESEAQWQALQIVHI
                     PWGQGRFLSSVELIK"
     gene            complement(11336..>11623)
                     /locus_tag="I9760_001992"
     CDS             complement(11336..>11623)
                     /locus_tag="I9760_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043755.1"
                     /translation="AVGVPSDQLRITYAASLTLNQAHMVLSFYPTPESEPLILDSLES
                     KILKASARPDLKPVYSFNAEGLWLAKTRGESSLMGDSKSLGKWDALMKRME"
BASE COUNT         3726 a         2283 c         2147 g         3467 t
ORIGIN      
        1 tttgagtgtt attttaataa ataataatgc tataagctat tagtaggtat cagttaccga
       61 aatatagggt acatcattta aatacattat gttagaatga aaaccaaaca aaattacgac
      121 tcaataacct atgaactttt gcacacttgg actagcagtt attgaaactg aagcacaagc
      181 tgtatttgag ctaactcaac gaattgatga acattttgaa aaagcttgtg aattactctt
      241 atcatgcaaa gggcgtatcg tcgtcacagg catgggaaaa tctggccata tagctaataa
      301 gctggcctct actttttcca gcactggcag ccccgctttt tttatgcatc ctggagaagc
      361 aagtcatgga gacttgggta tgataactcg ccaggatata gttgttgcca tttccaattc
      421 gggcaatacc catgaaatag ttactttgct tccattatta aaaagacttg aagtcccctt
      481 aattacattg accggcaata aacaatcaac tctggctaaa tctgccgacg tcaatctgga
      541 tgtcagtatt aaacaagaag cttgccctct gggtcttgct ccaacaacca gtactacggt
      601 atcgctggtt atgggggatg cattggctat atctctttta caagcacggg gattcagtgc
      661 agaagacttc gctctttccc atcctggagg cgcacttggc aaaaaattgt tgctaaaaat
      721 agacgattta tgtcatacag gagaacaact tcccttggcg aatgaaaacg caacagtgag
      781 tgacgcttta attgaagtca cgaataaaaa gctgggaatg acttgtgttg tagacaacca
      841 tggctattta gtgggtgtat atactgatgg cgatatccgt cgaacattga cacgtcaatt
      901 taatatcaac actactctaa tcaaggacgt catgactaaa aactgcagaa ctatttataa
      961 aggcatgttg gctgccgaag ctttagcaat catgcaaaaa tatagcatca catccttggt
     1021 agtagttgaa aatgataata gaccttatgc agtactccat ttacatgact tgctaaaagc
     1081 aggtgttttt taatacagag atagaaatga acgaactatt ggaaaaagcg aaaaaaatta
     1141 aatgtcttat ttgtgatgta gacggggtgc tcagtgatgg cttacttcac attgataatc
     1201 atggcaatga gctgaaatca ttccatgtac aagatggaat ggggttaaaa ttattaatgg
     1261 ccgccgggat acaagttgca atcattacca cagcacaaaa tgctgtagtt gaccatcgaa
     1321 tggagcaatt agggataact cattactata aagggcaagt tgataaacgt agcgcttacc
     1381 aacatctaaa aaaagcattg ggactcaatg atgaggagtt tgcctatatt ggcgatgatt
     1441 tgcctgattt acccttgata caacaagtgg gattaggagt tgcagtcagt aatgctgtac
     1501 cacaagttct ggaatttgct gattggagaa cagagcggac tggtggtaga ggcgcagttc
     1561 gtgaattatg tgacttaatt cttaacgcac aaaataaagc agaattggcg atcacaggct
     1621 atttaaaaca atgaatatta ccaagcaagc aatttggcta ttcttgacct tgcttatttt
     1681 agcaggatct ggttggtatt atgctcattt taatcaggct attatcaata agaaaacgtt
     1741 gtcaaatgaa gttgacacaa ccatttcgca gcttaccgta aagcaattta attcagaagg
     1801 tgtgttaatt aacttattaa ccactccaca ggtagaatat attcctaaca acaatctcca
     1861 tttgcttaaa aacccccata ttattattaa gcaagacaat caacctgcat gggaaatcaa
     1921 ttcattacga gcaaaatcgt ttgatggtgg taaacaaatt acttttatag agcaagtagt
     1981 agttcatcaa aatccgggag aaagcaccag ggaaagcact ttaaaaactg aagagattac
     2041 ttattatccc aaggaaaaaa aagcaacaac aagtcttttt gtaacctttg aacaaccagg
     2101 caatatcata cagtctactg gaatgaatgc ctatttggat gaaaaaagag tagaattact
     2161 ccataaagcc agaggtagct atgacccagc taaaggttaa atacattata ctactattgt
     2221 taatgttgtg ctccatttca ggaaatgctc ttccgtttga taaggaaaaa atggtgcaag
     2281 tagtttctga ttcagccgat ttaaatcaga aaaatcatca gggagtctat ataggaaatg
     2341 ttgagttcgt tcaaggttca accaatttgc gtgcagaaaa agctattacg caaggagatg
     2401 ccaaaaatca actcacttta gccattgctg aaggctctaa agacaagcaa gctcattatt
     2461 ggactacaac agcccctgat aaacccccat tccatgccta tgctgacact ataaaatatt
     2521 atccgctaaa acatttaatc gaattaattg ggaatgctcg catagagcaa ggaccaaact
     2581 ctctttcagc ggcaaaaatt agttatgata ctcaggaaca acatgtttta tcacatagtg
     2641 acgaaaaaac aagaacgaca atcattattt atcctgaaaa gaaatcatga gtttactaga
     2701 ggctaaacaa ttaaaaaaat catttaaggg ccgaacggtt gtgaatggtg ttagcattca
     2761 cattaagcaa ggtgaatgtg tcggtttatt gggtcctaac ggggcaggta aaaccacttg
     2821 tttttatatg attgtaggtc tgcaatcctg tgatgaaggt catattattc ttaatggagc
     2881 agacataact caagacgcaa tgcacctaag agcaaggcat ggtattagtt accttccgca
     2941 agaagcctcc gtttttagaa agcttactgt cgctgaaaat atcatggcga ttctggaact
     3001 aagaaaagat ttagatgatc aaaaacgtga agaaaagctg gatcttctct tacaagagtt
     3061 tcatatttct cacatagaaa acagcttggg catggcctta tcgggcggcg aaagaagacg
     3121 cgttgagatt gcaagagccc tggcaataga accatccttt atcttgcttg atgagccttt
     3181 tgccggggtt gatccaattt ctgtaatcga tatcaaaaaa attatcctgc atttgtgtga
     3241 taaaggtata ggggttttaa ttacagatca taacgtgaga gagactttag acatttgcga
     3301 acgcgcttat attgtaagtc agggaaaaat tctttgcgaa ggaaccccca aagcgatact
     3361 tgccaatcaa caagtaaggc aagtttattt gggtgaagaa tttgctttgt agcctcattt
     3421 aaaagaatac attttaatta tacttatgtt actaattatt gataactacg attcatttac
     3481 atacaacctg gtacagtact tccagagctt aaatcaaaaa gttgaagttt ttaaaaatga
     3541 cgaaattcaa atcgatacta tcaggcaatc agcgccgcaa tacctggtca tttcaccggg
     3601 ccccaactct cctgatgaat caggaatctc tatagaaatc attaaagaat ttcacaagca
     3661 tattccgatg ttgggtattt gccttggcca tcagtgcata gctcaggcat ttggaggcaa
     3721 aatcatccca gcaccagaaa tcatgcatgg taaaacctca gaaatcaccc atcattacca
     3781 gggcttattt acaggaatac caaatcactt tcaagcgacg agatatcatt ctttagccgt
     3841 ggatatcatg agcttgccca attgcttttc gatagatgcg tgggcagacg atacaataat
     3901 ggcaatatca catcgtcaat ttcctctttt tggtctacaa tttcatcctg aagccatatt
     3961 aagtcaattt ggcttgcaaa tactcgagaa ttttttacgc tatgaaaccc aaacttctat
     4021 ttgaacaact tctatcccga caggatttaa gctcagacca gatgcaagag gtcatccatg
     4081 cttgtatgac aggtgaattt agtgatgtgc aaattgcaac ctttctggca ttaatgcgca
     4141 tgaaaagtga gacagtcaat gaacttactg cagcagctaa agtcatgagg caattagccc
     4201 acaaaataga tttgggaaat cccctgattg atatagttgg cactggcggt gatggaagaa
     4261 atacttttaa cgtatcaaca gcctgtagtt ttgttgtagc tgcagcagga ataaaagttg
     4321 caaagcatgg taaccgttct gtttcgagtc gtagcggtag cgctgatcta ttagaacaag
     4381 ccggatttat acttaatctt tctgactcac aggtacaaaa ctgcatcaac caatgtcaat
     4441 tggctttctt gtttgcgcca cattatcacc ccgccatgca gcatgcgaga gctgcaaggc
     4501 aacaattagg aataagaact ttatttaatt tacttggccc actgattaac ccggctcagg
     4561 taaaacgaca agtcgttggt gtattttcaa cgaattggct caaaacaata gctactgtcc
     4621 tggccaactt aggtagtgaa agatcattag taataagctc tcaagatgga ctggatgaga
     4681 ttagtattgc agcgaaaagt gaagtagttg aataccgaga tggaaatttt aagcaatggt
     4741 tcatttctcc tgaagattat ggtctaaagc attcttcttt agatgcaatc atagtcgact
     4801 caccagagca aagcctgcat ttgattcaat ccgtactttc aggtgattct gggccagctc
     4861 gagatatagt ccttctcaat tctgccgcag ccatttattg tgccaaagat ggcatatcgt
     4921 ttgatgcatc catagaggaa gcaagaattg cgattgatag tggtaaagca aatcattgtt
     4981 ttaacaaact ccgattattg acccaaactt taaataaaga atcaaatcat gaatagtatc
     5041 ctggaacgaa tagccaaaca taaactagaa gaagttgcag ttgcgaaaaa aaataagcct
     5101 ttacatgttc tgtcaaaaca acaaccgggg gaaatacgtg acttcataac tgctttaaaa
     5161 tccaatacct cacctgcagt catagccgag ataaaaaagg cttctccaag caaaggtcta
     5221 attcgaaaag attttaatgt tgcagaaatt gccaagattt atacccaaaa tggcgcaaga
     5281 tgcttatccg ttttaacgga tattgagttt tttcaaggtc atcctgatta tttggcttta
     5341 gcaaaatcaa aaactacttt gcccgtattg cgtaaagact ttattattga ttcttatcaa
     5401 atctatgaaa gccttgtctt gggtgctgat tgcattttac tgattgtcgc attattagat
     5461 gatgttcaat taatggattt ttgccaactg gctcaagaac ttaaaatgtc tgttttggtt
     5521 gaaagccaca cgcaagatga attagaaaga gcattacgct tacccacccc tcttataggc
     5581 attaataacc gcagcctaca caactttaaa acagacattc aactgagtat ccaattaaaa
     5641 caatttgttc ctaaagataa aattattatt actgaaagcg gaatcaacac ccgtgaagac
     5701 ataaaattaa tgcaatccca tggcattaat gcctttttga ttggagagag tttaatgagg
     5761 gcagataaca tcgggaaagc attacagaaa ctaatgactg actaaataaa ctactcaaaa
     5821 cctggctcag gagtttgact tggctcaggc tcaggcaatt cgggataacc aggctccaga
     5881 ggctctggat tatcagggta ctccaaaggt tgttcagggt actctgatgg ctcctctgga
     5941 tactcaattg gctgttctgg tatttcagtt gtattcaaag tatcgcctcc ttctgatagt
     6001 tctattcaaa gtatagcaaa atcaatttta gcaatcattt tggtaaatac tcttgcccat
     6061 gatacacttt ttcataaaat ccacattaga aacctcaatg cctccctagc gccacagaaa
     6121 caggacttac gaaaaactcc aaggccaagg caaaaaaagt ttttaatgag ggagtttaga
     6181 taaactaaat gaccgaatta aaaacttttt ttaacgaaga gattggggtt tttcgtaagt
     6241 cctgaagaag agggcattaa acttttgtaa ggtattttat atgaatgtag taattatcac
     6301 tggtgcagca agtggcattg gacatgccgt aagtcaagtt tgtcttggaa gtggaaaaac
     6361 tatagtcatg gttgataaga atcaggacaa attacataca gaagcccaat cattcatgac
     6421 taaattctct cctgaacatg ttatttgttt tgcctgcgat gtaacccaac catcagaagt
     6481 agaacgactt gcagaatatt cctgtaaaca actaggccag gtagactgga tatttaataa
     6541 tgctgggatc attggcaatt tacttcctgc ctgggaactg caagaaaatg atattaatca
     6601 ggtaatgcaa gtaaatttgc atgggatgct taatataatt cgctcattca tgccttattt
     6661 attcaaacag aatttccgtt ctcatattat caacatggca agcctgtatg ccttatgtac
     6721 aggctcacaa atggccgcct attccatgtc aaaacatgcc gtattagcct tatccgagtc
     6781 attatatttt gatttaaatc gccttaaaaa acctgtagat gtttctgtag tatttccttc
     6841 ttttacagat acctcgttat tagcgagtag tgggaaatta aaccattctc caatccacaa
     6901 tcaattaaat aacttgttag cacattctcg acccgccatt gaagttgcag aacatatagt
     6961 aagggaagtt gaacaaaaaa gattttatat attgcctgat aaagaagtta aagggtattg
     7021 tgaagacaga accaaagcca tcctgttgca ggaaaatcct catcgtaaca gcgtcgaaca
     7081 attaatgtgt tctttattaa agcgccaatc atcagaagcc aatctgtaaa ttgtttgtga
     7141 ttttcaaaac agaataagtg taacccggct tataactgat gtgagcatcc atgctttaac
     7201 attcatgtac tcataacgaa tctagggata gaatttcagt ctcttatgtt tttggcctag
     7261 catttctaac aattcttttc tgaaaatatg ataatcgacc tctagagcat ggcgcatgtg
     7321 cgatttaaat cggtgatggg gtttattgat ttctttggct atttttcgat gtaattgatc
     7381 aggtcgtttc aaagtccctg caataatgcg agctactatt tttgattgat aatcagccaa
     7441 aggccaaatg caaccttgag gttggcatag gccaatgaaa tacaaatttt caaactcggg
     7501 gtgcatcatt tttcgatata aagggatggt ggtagagtta ctaaaatcaa tacaactttt
     7561 atcaaaaaat ggaaaactga tttggtaacc cgtagcgaaa atgatcgtat cgaactcccc
     7621 ctgggttcca tcagtaaaat gaacggtgtt accctcaaat ctcgatatac cgggacgagg
     7681 gtgtattttg ccatggcgaa taaaatacaa gagttcggaa ttaatagtag ggtgaatttc
     7741 caatggccca caatcaggct tcattaattt atacttggca taccggcctt gcaatcctct
     7801 aatgaccagg ctgatgaact tctgtcttag ccaggatggc atccattgta ttttagctac
     7861 cgctacatcc gttggcttac caaaaataaa tttggggaaa atatgataac ccctacgcat
     7921 gctaatgcaa gttccaggag caactcgcga tatttcaaca gcgacatcac aggctgaatt
     7981 acccccgccc acaaccaata ctcgcttgcc tttaaaaatt gaagcttttt tatattggtg
     8041 agaatgaagt atctgcccgt caaatactcc aggatataca ggcataaagg gatcccaatg
     8101 atgaccatta gctaccagca aatagtcata gcacgcttca tgaattcctt gggcatcctc
     8161 atagataaca tgccatgtat tatcatcaag tcgatgtacc ttttgtacca ctgtgttaaa
     8221 acgaatgtat ctatctaaat gaaaatgctc cacataactc tgaaaatatt ttaaaagctg
     8281 ggagtgtgaa ggataatccg ggtaatcaac aggcataggg aaatcttcaa actcagacca
     8341 gcgttttgaa ctaatgatat gagtcgtctc ataaacgctg gaatgctcat tttgctcatc
     8401 ataaacccaa ttaccaccta tttgattgtt tttttcaaag acagtaacat tagtaatgcc
     8461 gtgttcttgc aaatttttaa tggcagcaat accgctggga ccagcaccga tcacacaaac
     8521 acgggcagac agacggtcct taagcaccct ttttatttcc atcaactaac accttatcaa
     8581 ctttatccat tatcatcgct tgtaaaattt cgtctttgtc aattgtaaaa tgatttactt
     8641 ttatcccttt tatcgaatca acattgatat tatcaatctt tgtcaattga ggatcgactg
     8701 ctctgagttt taacccgcta aacatcggta caaaaccagg tttatataca ttcaaagctt
     8761 gtttaacatt aggaggcaca atagtttgtg atactgcatc gacagtcact aacaaagcaa
     8821 cagggacacc tgcggcattc aaatttcgag ccactttgat ttgttcatta gcccctaaag
     8881 aatgccccac taaaataatt ggcttgtgat ctttctcttg atagtaatgg tcaataatat
     8941 accgacttac cgctccagcg ttataccaca tggtgctatg agccgtgata tggtattttt
     9001 cagacacttt atcacgaagt gtgtacatcc ccttgctgaa aattcccagg ccgccaagca
     9061 tagtatggac ttcgcctttt tttgatgaca tattttgtgc tttggacttt ttgttaggga
     9121 catcagaaat tgcaggttgc ttttctttct tgatataagt atttcgcgcg cccgtcaaat
     9181 ccacacaagc cgttaaacta ctaacaatca acaacaataa aaatgatttg gaaaaacgta
     9241 tttgcagttt gccaaaatag ttcatattaa tattattatt gtaattattt gattttttat
     9301 cataccttat tcttgtacca ttttacagag gcacctggcg ggtttaaggc tattcgcgaa
     9361 ttcattttaa tgccttgaga ataatctgtt tttcatctcg ttattttatt aattctacag
     9421 aagataaaaa gcgtccctgc ccccaaggta tgtggactat ttgtaatgct tgccattgag
     9481 cctcgctttc cacttgagtt gctaccaccg caatatccat cgttttagcc atttcattaa
     9541 aataatgtat gaaaaactgc ttgctttcat tttcatcaat atctgtcacc agactcccat
     9601 gcagctttaa ataatctatg tttaaatcac tcaaataatg caacggagaa aatttaatac
     9661 ccacacgatc tattccaact gtcaccccca attttttggc ttgcttgata aagaacttgg
     9721 agtttgaaaa atgtgacaaa acgtgagcct cattaatttc cagtgaaaga tttctcaaga
     9781 cagctgcagg agtgtcttcc aattgacgca aataaccagt gctattgact ttatttgcta
     9841 aagtatcttc tgaaatatta agagcaaaat gggtatgtgt tgcaatgtcc atgacggtta
     9901 attcattgag cacatactga tcaataggat aggccaatcc taatttctca gcaactggta
     9961 taaaataacc agcgcctaac tcctcccctt cctggttgcg aattcgaacg aagacctctt
    10021 tatgaaaaca attcttaccg tctgtaactg cctgcgcata caagcttatt tttttctgtt
    10081 gcaatgagtt tctaatttca tcgccgctga ttaactgagg atatttataa gtatcatgct
    10141 ctttctggca ataaaatacg ccggtttcac gagccttttt aaccgcttga tctaccgtag
    10201 acaacagatt ggagacaggt tggtggagaa aataactcgc agcccccatg tgtattttgc
    10261 aaattttaat atcattgata ccttggctca aaatttgttc aaactcctga cattcctttt
    10321 caaacaccaa aggatccctt tcatgactaa ttaaggcaaa ggtcgtcccg ttaattcttg
    10381 ccaatgtact aacagacgat tgcttccaat agcttttaca tattttggct acagttaata
    10441 ccaattgatc cccttgctga tacccttgct tctgatttaa ctcatccaga ccgtctatga
    10501 ccagcatcat gacataacca ggtataaatt catcttcatt atccagaatc aaagccaaat
    10561 gctgtaaaaa ataacgacga ttactcatcc ctgttaatga atcttggtaa acctgtgttc
    10621 taagtgattc tgtttgtttt aattggtctt gaaataagga tttgacttta gtaaccattt
    10681 gattcatagc caaggtaact tgcctcaatt caggagtttt aggaatcttc gtttctacag
    10741 gaaactcatg ttctgaaatt gccaaagcct gtgctgtgac tcgttttaaa ggttgcaata
    10801 aatattttag aaagccatag cttagaacca aagcaactaa cgcaaaaatc agataagcgt
    10861 ttaccatttc tacagcatta cgccacaaag aggcgtaaac atagctcgga tcactagcga
    10921 ctaaaacaac tcctgcttgc atccacccat ccataattaa ggatgacttc tcagtcgagg
    10981 gccattttat caagttcaca aaccattgtg gtatatcgct ttcttgtggc aattgtttct
    11041 ttaaaatgat cactttccct tttatgtctt gtactttaat tgatgaaaaa tatcctctgt
    11101 caaaaacagc tttaaccata gaatccatgg taggtacatc atgattgatt aaagactgag
    11161 ataaagacaa acctaatgat gtcgcagtat cttgagcatt actttcaagt tgttggataa
    11221 aaaaattacg cgcattattc atagtgatga aataagttcc gataaaaacg aataacaaca
    11281 taatcaacac gcctacagcc attttcttag ttaatgtcat ggtctatcac cttttttatt
    11341 ccatacgttt cattagagca tcccacttgc ccaaactttt tgaatccccc atcaatgaac
    11401 tctctcccct tgttttcgca agccataaac cttccgcatt aaagctataa actggtttca
    11461 aatcaggcct tgcagaagct ttcagaattt tactttccaa actgtcaagg attagtggtt
    11521 cggattcagg agttgggtaa aaagacagca ccatatgtgc ttggtttagt gtcagagaag
    11581 cagcataagt gattcttaac tggtcagaag gaacaccaac agc
//
LOCUS       DACTJE010000099        11565 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.099, whole genome shotgun sequence.
ACCESSION   DACTJE010000099 DACTJE010000000
VERSION     DACTJE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11565
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.099"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1550)
                     /locus_tag="I9760_001993"
     CDS             complement(<1..1550)
                     /locus_tag="I9760_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HAT5043756.1"
                     /translation="MLVESVIGIVRAVNGLLEKVNAQGQASLVKSGARLQEGDVLTLL
                     SGEAYIQFIHGFPEALALGKPIKLDGVSPTLQNGVEDLNEQLVQEAIAKGIDPSVILD
                     ILGSAAAGAVAVGSGGDAFIIDPLFGFGQVTAGYPTGPISFAYEADTQHLFWYVPEEA
                     IAESESTIEPESLPQIPQFNTNQATVTVFEDALPSGGIRDSAEQTTTARASLSPLISS
                     SSEVASSFAFNTDLSVLPKLKSGGLELEYVLSSDRLALSASTPDGVDVVRFELTADGQ
                     LTQILIGPIDHSRADSDDSEWHRLDLSPAINVTFTRISDGTFLGSRTLPASAAVAEIQ
                     DDVPIARAQLTNNEILLDETIGMKVGDADAANDDFNPTTTVDPFNNIYGTPLGLVQNA
                     NLLDASTSEMGGDYKNATMTHLLKITDAVSGLQTTDGTPINLFLESNGDITGRAGDVG
                     APAVFAIRMNPNTGAISVAQYGPIKQFDTNSHDEAVDLTGRISAVVTAKDSDGDESNA
                     EIPIGQLI"
     gene            1962..3200
                     /locus_tag="I9760_001994"
     CDS             1962..3200
                     /locus_tag="I9760_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HAT5043757.1"
                     /translation="MNWKDKIKTKFYSCDRFVRFVIQHFIQDDCTYIASALAFTSLLA
                     VVPLMSVGLAIFSSFPVFKGLAEPVQNFIFDNFVPATGKIVQSYLQQFTSQVSKLSIW
                     GIVFLICTALLVMFTIERAMNKIWRVSSSRHGVSAFLLYWAIISLAPVLLGLSLAASS
                     YLFSMPILADHRAPYTILHYSPFFLSLIGFTFLYVVVPNCHVKIRHAFWGGLVAAILF
                     ESAKHAFAYYLIRYNTYALLYGAFATVPIFFIWVYWVWIITLLGAEISYAFSVHHQRR
                     GGKSLDGFSHALLWLHQLWLAQQHGKGLSFNDLVDASKQPFAVDVDEMINALIYHELI
                     HATADGHYMLSRDLSHVTLYDLTQLLPYRLPTHLELQYSKASLAEQWRAAFKRHNEEL
                     KKSLDINLEELFKKTGAVLK"
     gene            complement(3235..4809)
                     /locus_tag="I9760_001995"
     CDS             complement(3235..4809)
                     /locus_tag="I9760_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HAT5043758.1"
                     /translation="MKTQRIIHPNIDIRKFPEVNADFSMTDISMGDLHANALLFLNIL
                     VRQGIIAISPENYAKFAEIYTLPELQADYWGTEAPVFSAGNKQERLEEIKKQYNALIA
                     QIKIINTKKLIRLIGDELVDRGVIDYFILKLLQALHDQGAGFEILLSNHGIEFVEACE
                     LFKENGNKLVAKRLGNIQHGNSFHALQEAIAAGAISNEEVLNIYHQVYKKHLKIISYS
                     LDPEANEIKVFSHAGIGLNHIRGLARKFKVPYSEESAVDLAKTIDAINKKFAEKASAG
                     EIHTLYTHDMMYRGYAGEYLNSTDEVVAATVWGREYGDLIRTSKKFKVTFIHGHDSYD
                     PEKVEHVTLNNQLGQFQNNVGDLYLYATNGLRAVPIQYLNPDKKILSSREKNQPANAN
                     DYVVKIPHTKPSFFKITNPMMTFPDSYKQIWDSTKGGSNIARIKALLKDYTKEDSVFG
                     SFWGLIFTLHWGRHHVKSVHQIAQTRYTSVEAILSDLKALKPKEGGSLDKRIKFIESQ
                     IRTQRCNNPDLQFNLK"
     gene            complement(5009..6328)
                     /gene="hslU"
                     /locus_tag="I9760_001996"
     CDS             complement(5009..6328)
                     /gene="hslU"
                     /locus_tag="I9760_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAT5043759.1"
                     /translation="MTPREIVQELDKHIIGQDDAKRAVAIALRNRWRRMKIKDPVLRN
                     EIMPKNILMIGPTGVGKTEIARRLANLAKAPFIKVEATKFTEVGYVGRDVDSIIRDLT
                     DMAIKQEREFAMKKVEHLAEDAAEERILDVLLPPARGTLTPGEKNTTARQVFRKQLRE
                     GELNDNEIEIEVAATPVGIEIMAPPGMEEMTSQLQSMFQQVGSYRTKTRKMTVAKAMK
                     ILREEEAAKLINEEDIKLKAIESVEQNGIVFIDELDKIAKRSDTVSGGDVSREGVQRD
                     LLPLVEGTTVSTKYGMVKSDHILFIASGAFHVAKPSDLIAELQGRLPIRVELSALSVE
                     DFVRILTEPSASLTLQYSALMETEGLTLTFDETGIRRIAEVAWQVNERTENIGARRLY
                     TVMERLLEVVSFEATDKAGETVHVDKAYVDKNLGQLIADEDLARYIL"
     gene            complement(6336..6884)
                     /gene="hslV"
                     /locus_tag="I9760_001997"
     CDS             complement(6336..6884)
                     /gene="hslV"
                     /locus_tag="I9760_001997"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAT5043760.1"
                     /translation="MEQFHGTTILSVRRGNQVVIGGDGQVTLGNTVMKGNARKVRRLY
                     KDKVIAGFAGGTADAFTLFERFEAKLEMHQGHLIRAAVELAKDWRTDRILRRLEAVLA
                     VADSKASLIITGNGDVIEPEESLIAIGSGGPFAQAAARALMENTQLSAKEIVQKALTI
                     AGDICIYTNNNLTIEELNDEGK"
     gene            complement(7088..8311)
                     /locus_tag="I9760_001998"
     CDS             complement(7088..8311)
                     /locus_tag="I9760_001998"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAT5043761.1"
                     /translation="MTGRVCVLVMDSFGIGASLDAARYGDAGANTLVHIYEACKRGEC
                     DIDGVRKGPLMLPNLAGKGLYHAAMASSGLPFIDLSALAIPSGYYGYAVEQSLGKDTP
                     SGHWEMAGVPVTFEWGYFPDKSYCFPEELISEFIKQCNLPGVLGEKHASGTIIIDELG
                     EEHIRTGKPIVYTSADSVFQIAAHEEAFGLQRLYDICKIARNLVDKYQIGRVIARPFT
                     GKPGSFKRTGNRKDYATPPPEKTLLDFLKEDGREVIAIGKIADIYAHQGVTQEIKADG
                     NMALFDATLSAMKTAPQGSLVFTNFVDFDSSYGHRRDVAGYAHALEQFDVRLPELEAL
                     LQPDDMVFIAADHGCDPTFPGSDHTREHIPVLMFGPQVNSKFIGRRDCFADIGQSIAE
                     HLQLSSPLTHGVSFL"
     gene            complement(8308..9591)
                     /locus_tag="I9760_001999"
     CDS             complement(8308..9591)
                     /locus_tag="I9760_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5043762.1"
                     /translation="MQVVDEFKAFDVKASLMPWLVCFAATMFFFYEFIQGNMFASIAA
                     NIMQDFQIQADKMAYLSSIYYLANVIFLFIAGMVLDRFSIKNTILIAMFLCVISTFIL
                     SYSDSFYVALFCRFVTGIGSAFCFLGPVRLASHWFPPQRMALVTGAIVTVAMTGGMLA
                     QYPLTKLVLYVGWRQAVQDVGILGLAMLVLMFFWIKERPQVAIKKEGKPINVLVAARK
                     AYLNAQNLRAALYTSLMNMAIAVFGAMMGTLYLEQRLGISPDQAAMINGMLFFGAIIG
                     SPLMGWISDKVGLRVLPMKGGVLASLLILLGVLYLPVSVTMMAVLFFLLGLFTSAQVI
                     SYALVAESSPPMMTATAVSVVSILTQGGYLIYQNLFSALLTNYGDVRLINGVPVYLLE
                     AYQHAAIILPIGLLIALLLLFGLKETRCQQVEHTV"
     gene            complement(9604..10872)
                     /locus_tag="I9760_002000"
     CDS             complement(9604..10872)
                     /locus_tag="I9760_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5043763.1"
                     /translation="MSHSVAVESRTFVPRGDFMAWVVCLSAGLFFLYEFFQLNIFDVI
                     NQSLREDFHIDATQLSWMSSTYLWADILFLLPAGLILDRFSTRKVILTAMLVCVVGTI
                     GFAVTESFFLASFFHFLSGIGNAFCFLSCVVLVSHWFPPRRQALVIGSLVTMAFIGGM
                     MAHTPFAYLNDLFGWRRALLIDGVVGAFLILWIYMIVQDRPEESPAHKLTNEGQILSS
                     FMKALSNKQNWLAGLYTSLLNLPIMVLCALWGASYLQVAHHLPDIAASNVVSLIFMGS
                     VVGCPLVGWLSDTQGRRKPLMIFGAIATLITTIPLFINVVLTQMSLSILFFALGLFTS
                     TQVISYPLVAESNQLENTGAATGIASVIIMGGGGVAQVLFGWLMTHHAGTNVTAYTVS
                     DFQFAMWMFPVAAIAGLVAVLMTRETYCKR"
     gene            complement(10923..11552)
                     /locus_tag="I9760_002001"
     CDS             complement(10923..11552)
                     /locus_tag="I9760_002001"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAT5043764.1"
                     /translation="MAKLYFYYAAMNAGKSTVLLQSSYNYRERGMQTLLFTPAIDTRF
                     QYGTICSRIGVSEQAYAFNNSDNLYVLTQEFQLQTQKYSCVLIDEAQFLTREQVYQLT
                     EITDQMSIPVLAYGLRTDFRGELFPGSQFLLAWADELIELKTICHCGRKAIMNMRIDE
                     NGQAVIEGEQVLIGGNESYVATCRLHYKRGEAGKTFPRNKLFNKDTNTF"
BASE COUNT         3534 a         2512 c         2077 g         3442 t
ORIGIN      
        1 ataatcagtt gtcctattgg tatttctgcg ttagattcgt caccatcgct gtctttggcg
       61 gtaacgacgg ctgaaatgcg tcctgttaaa tcaactgctt catcatggct gttggtatca
      121 aattgtttaa ttgggccata ctgtgcaacg ctaattgcac cagtatttgg attcatgcga
      181 attgcaaaaa ctgcaggagc accaacatct cctgctcgcc ccgtaatatc accattcgat
      241 tccaaaaaga gattgatagg agtgccgtca gttgtctgta atcctgaaac agcatcggta
      301 atttttaaca aatgggtcat tgttgcattt ttataatccc cccccatttc agaagtactg
      361 gcatcgagga gattcgcatt ttgtaccaaa cctaagggtg taccataaat attattaaaa
      421 ggatccacag tagtcgttgg attaaaatca tcattagctg catcagcatc accgactttc
      481 attcctatgg tttcatccag caagatttca ttattggtca attgagccct tgctattgga
      541 acatcgtctt gaatttctgc caccgcagca ctagctggta gcgttcttga ccctaaaaaa
      601 gtaccatcgc ttatccttgt gaaagtaaca ttgattgcag gtgagagatc taatctatgc
      661 cattcgctgt catcactatc agctcttgag tgatcaatcg gtccaattaa tatttgagtt
      721 aattgtccat ctgctgtcaa ctcaaatcgt acaacatcca caccatcagg agttgatgct
      781 gatagggcta gacggtcact agacagaacg tattcaagtt ctaaaccacc tgactttaat
      841 ttaggaagta cacttaaatc ggtattaaaa gcaaaagaag aagcaacttc tgaggaagag
      901 gaaattaaag ggctcaaaga tgctctcgca gttgtagttt gttctgctga atctctaata
      961 ccaccacttg gcagggcatc ttcaaatact gttacggtag cttgatttgt attgaactga
     1021 ggaatttgcg ggagagattc aggttcaata gttgattcag attctgcgat cgcctcttcc
     1081 ggtacatacc aaaataggtg ctgagtatct gcctcataag caaaggaaat agggccggta
     1141 ggatatcctg ctgtgacctg tccgaaacca aacaagggat cgataataaa agcatcaccg
     1201 cctgaaccaa cagctaccgc acctgctgct gctgagccga gaatatctaa tatgactgaa
     1261 ggatcaatac ctttggctat cgcttcctga accagttgtt catttaaatc ttccacacca
     1321 ttctgcagtg ttggtgaaac accatccaac ttgatcggtt ttcctaaggc tagtgcttca
     1381 ggaaatccat gaataaactg gatgtaagct tcaccactta ataaagtaag tacatcccct
     1441 tcctgtaatc gagcgcctga ctttaccaga cttgcttgtc cctgagcatt tactttttca
     1501 agcaatccat tgacggctct cactataccg ataacagatt ctaccaacat gtttcgtcct
     1561 caaaatttac tattattcat attaataata gttcaatgat cacacacggt ggtggtataa
     1621 atgtaacaaa attgtaaact tttgaatcat attgatataa ttatatcttg tttttatagg
     1681 tttttttgct ttaacagttt tgatatatgc tattgactta cttagattgg atagagcaat
     1741 tgaatgatca caaagttgta aagaagtatt atattatcga taaattggat gattagataa
     1801 ataattttct atccaactca attgttcatg agagtttaga caacctaagt gatcgaatta
     1861 aaaatatttt aaacaaagag attggagttt tgcaagtcct gactatacaa aaatgggcat
     1921 aaaacgatat agttatacaa taaaaatctt ctatttaatc catgaattgg aaagacaaaa
     1981 taaaaactaa attctattcc tgcgatcgct ttgttcggtt tgttatccaa catttcatac
     2041 aagacgattg tacctatatt gcctcggcat tggcctttac aagcctgctg gccgttgtgc
     2101 cattgatgtc tgtgggtttg gcgatatttt cttcatttcc cgtattcaaa ggtttggcag
     2161 agccggtaca gaattttatt tttgacaatt tcgttccagc tacaggtaaa attgtacaaa
     2221 gctatttaca acaatttact tctcaagtat caaaactctc aatttggggt atagtttttc
     2281 taatttgtac agctttattg gtcatgttca ctattgaacg agcaatgaat aaaatctgga
     2341 gagtgagttc atctcgacat ggtgtttccg cctttttact ctattgggct atcatctccc
     2401 ttgctcccgt gttgttaggt ttgagcctag cagcaagctc ttacttattc tcgatgccaa
     2461 tattggcaga tcatcgagct ccttatacta ttcttcacta ttccccattt tttctttcac
     2521 tgattggttt tacctttttg tacgtggtgg ttcctaattg tcatgtcaag atccgtcatg
     2581 ctttttgggg tggattggtt gccgctatct tgtttgaatc agctaaacac gcatttgctt
     2641 attacctgat acgttataat acttatgcat tgttgtatgg agcgtttgcc acggtaccca
     2701 tcttttttat ttgggtttat tgggtgtgga ttattacttt gttaggggct gaaatcagtt
     2761 atgccttttc agtacatcac cagcgtcgcg gtgggaaatc tcttgatggc ttttcgcatg
     2821 cattgctctg gctgcaccag ttatggctag cgcaacaaca tggaaaagga ttaagcttta
     2881 atgatctggt ggatgccagt aaacaacctt tcgctgtaga cgtcgatgaa atgatcaatg
     2941 ctctgattta tcatgagctg atccatgcta ctgcagatgg gcattacatg ttaagtcgtg
     3001 atctcagtca cgttacgctc tatgatttaa ctcaattatt accttatcgc ttgccaactc
     3061 atctggagct gcaatattcc aaagcttctc tggctgaaca atggagagcc gcctttaaaa
     3121 gacataatga agaattgaaa aaatccctgg atattaatct ggaagaatta ttcaagaaga
     3181 caggagcagt actcaaataa cgataactta agtgaaaaag acaacacaaa aaaactattt
     3241 caaattgaat tgtaaatctg gattattaca cctctgagtc ctaatttgtg attcaataaa
     3301 cttaatgcgt ttatccaatg aaccgccttc ttttggctta agtgccttca agtcagatag
     3361 aattgcttct acagatgtgt atctggtttg tgcaatctga tgcaccgatt tgacatggtg
     3421 tctcccccag tgcaatgtga atattaatcc ccagaacgag ccaaagacac tgtcttcctt
     3481 ggtataatct ttaagtaatg ccttaatcct ggcgatattg gaaccccctt ttgtactatc
     3541 ccatatttgt ttgtaactgt ccgggaatgt catcatggga ttggtaattt taaaaaatga
     3601 tggttttgta tgaggaattt ttacaacata atcattagca ttagctggtt gatttttttc
     3661 ccttgaactc agaatttttt tatctggatt taaatattgt attggtactg ctctcaagcc
     3721 attggtagca tagagatata aatcacctac attattttgg aattgcccta gttgattatt
     3781 taaagtgaca tgttctacct tttcaggatc gtaactatcg tggccatgaa taaaggtgac
     3841 tttaaatttt ttagacgttc gaatcaaatc cccatattca cgtccccaga ccgttgcggc
     3901 aaccacttcg tctgttgaat ttaaatattc acctgcataa cctctataca tcatatcatg
     3961 ggtataaagc gtgtgaattt ctcccgcaga tgctttttct gcgaattttt tgttaattgc
     4021 atcaattgtt ttagccaaat caacagccga ttcttctgaa tagggtactt taaattttcg
     4081 ggcaagccct cgaatatgat ttaaaccaat accagcatga ctaaacactt taatttcatt
     4141 tgcttctgga tccaatgagt aagaaataat tttcaagtgc tttttgtaaa cttgatggta
     4201 aatatttaat acttcttcat ttgaaatcgc accagcagct attgcttctt gcaaagcatg
     4261 aaatgaatta ccatgctgaa tatttcctaa tctttttgct actaatttgt ttccattttc
     4321 cttgaatagt tcacaggctt cgacaaactc aatcccatga tttgaaagta atatttcaaa
     4381 accagcacct tgatcgtgca aagcttgtaa taattttaaa atgaaatagt caataacacc
     4441 gcgatctact aattcatcac ctatcaagcg aatcaatttt ttagtattaa taattttgat
     4501 ttgcgctatt aatgcattgt attgtttctt gatttcctcc aaacgctcct gtttatttcc
     4561 agcgctaaaa acaggtgctt cggtccccca gtaatctgcc tgaagctctg gcaaagtata
     4621 aatttcagca aatttggcat aattttcggg agaaatagca attattccct ggcgaacaag
     4681 aatattgagg aaaagcaagg cattagcatg caaatctccc atgctaatat cggtcatgga
     4741 aaaatccgca tttacttcag gaaattttct aatatcaata ttgggatgga taattcgctg
     4801 agttttcatt tatttctaat ataaacaacc attatgtaca taatgatatc agaatataat
     4861 tagcattgcc ttgctctttg taaaagtaaa ttgcatggtt gtacactcag aatcacatga
     4921 gggatatctc tttttcgaga aatatccccc acaaaaagtt gatctgttaa aaagatttca
     4981 ttcttgattt cagtttaact attctggatt acaaaatata gcgagccaaa tcctcatcag
     5041 cgattagttg gcctaaattc ttatcgacat aagctttatc aacatgcact gtttcgccag
     5101 ctttatcagt cgcttcaaat gaaaccactt ccagtaagcg ttccataacg gtatataatc
     5161 ttcttgctcc aatattttcc gtacgctcat tcacttgcca ggctacttca gcaatacgtc
     5221 gaattcctgt ttcatcaaag gtaagagtca atccttcggt ttccatcaaa gcggaatact
     5281 gtaaagttaa agaggcgctt ggctcagtta ggattcgtac aaaatcttcc acagagaggg
     5341 ctgacaactc gacacgtatc ggcagacgcc cttgcaattc agcaatcaag tcagaaggtt
     5401 tagctacatg aaaagcacct gaagcaatga ataaaatatg atcagacttt accatgccgt
     5461 attttgttga aacggtagtt ccttcgacta atggcaataa atctcgctga actccctcac
     5521 gtgatacatc tcccccactt acagtatcag agcgtttggc aattttatcc aactcatcaa
     5581 tgaacactat tccattttgc tcaacacttt caatcgcttt taacttgata tcttcttcgt
     5641 taattaattt tgccgcttct tcctctctca aaattttcat tgctttggca acggtcattt
     5701 ttcgagtttt cgttctgtag cttccaactt gttgaaacat agactgcaat tggctagtca
     5761 tctcttccat acctggagga gccataattt caatgccaac aggcgttgcc gctacttcaa
     5821 tttcaatttc attatcatta agctcacctt cgcgcaattg cttacgaaac acttgacgag
     5881 ctgtcgtgtt tttctcacca ggagttaatg ttcctcgagc aggagggagt aaaacatcca
     5941 ggattcgttc ttcagccgca tcttctgcca ggtgctccac ttttttcatg gcaaattcac
     6001 gctcttgctt gattgccata tctgttaagt cacgaatgat cgaatcaaca tcacgcccta
     6061 catatcccac ttccgtgaat ttggttgctt ctactttaat aaatggagct ttagccaaat
     6121 tagccaaacg tcttgcaatc tccgtcttac caacaccggt agggcctatc attaaaatat
     6181 ttttaggcat tatctcgttg cgcaatactg gatctttgat tttcattcga cgccagcgat
     6241 ttcgtaatgc aatggctacg gcacgttttg catcatcttg accaataatg tgcttatcta
     6301 attcttgaac tatctctcga ggggtcatga ccatattatt tgccttcatc atttaattct
     6361 tctatggtta aattattgtt agtataaata caaatgtcgc cagcgattgt taaagctttc
     6421 tggacaatct ccttggcaga caactgtgtg ttttccataa gagctctggc tgcagcttga
     6481 gcaaaggggc caccagagcc gattgcaatt aaactttctt cgggctcaat gacatcgcca
     6541 ttaccggtaa taattaaaga agccttgcta tccgctaccg caagcactgc ctctaatcga
     6601 cgaagaattc tatcggttcg ccaatcttta gccaattcaa cagctgctct aatcaaatgc
     6661 ccctgatgca tttccagttt tgcttcaaac cgttcgaaaa gagtaaaagc atctgctgtt
     6721 ccacctgcaa aaccagcaat cactttatct ttatacaatc tccgaacttt acgtgcattg
     6781 cctttcatca cggtattacc tagagtaact tgcccatcac cgccaataac aacctgatta
     6841 ccacgtcgta ctgaaagtat tgttgtccca tgaaattgtt ccaaaataaa atcctcaatt
     6901 acattgcaga aatggctaaa tggggatcat tggtaaaaaa tcaataggga ttgagagaaa
     6961 aattaagtgt tcttaatatc tcaaaatatt aatcgtcatg tccactccct tacggtcgtg
     7021 gctcggattg tcgtgactcg gattatcgtt caaaccgagc cacgaccata agggagtgga
     7081 tataaaatta taaaaaagaa acaccatgag tgagtggcga ggataattgc aaatgctccg
     7141 caatactctg accaatatct gcgaaacaat ctctccgtcc aataaatttg ctatttacct
     7201 gagggccaaa catcaatact ggaatatgct cgcgggtatg atcagaacca gggaatgtag
     7261 gatcacatcc gtgatctgct gcgataaaga ccatgtcatc gggttgtaat agagcttcca
     7321 actctggcag acgcacatcg aattgctcta gggcatgcgc ataaccagct acatcacgcc
     7381 tatgcccata agaagaatca aaatcaacaa aattggtaaa taccaagcta ccttgtgggg
     7441 ctgttttcat tgcggataaa gtggcatcaa acaaagccat attgccatcg gctttgattt
     7501 cttgcgtaac cccttgatga gcatagatat ccgcaatctt acctatagca atcacttccc
     7561 gaccatcttc ctttagaaaa tcaagcaatg ttttttccgg aggaggtgta gcgtaatctt
     7621 ttctattacc tgttctctta aatgagcccg gcttacctgt aaaaggacga gcaatcactc
     7681 gaccaatttg atatttatca accaaatttc gagcaatttt gcagatatca tagagacgtt
     7741 gtaaaccaaa tgcctcttca tgtgcggcaa tttgaaatac gctatcggca gaagtatata
     7801 cgataggctt accggtacga atatgctcct cacccaactc gtctataatg atggttcccg
     7861 aagcatgctt ttctcccaaa actcctggca aattacactg cttaataaat tctgaaatta
     7921 attcttcagg gaaacagtaa gatttatcag ggaaatatcc ccactcgaaa gtcacaggca
     7981 ccccagccat ttcccaatga ccactggggg tatctttgcc caagctttgt tccacggcat
     8041 atccataata tccagaagga atagctaaag cagataaatc gataaagggt aacccggaac
     8101 tggccatagc agcatggtaa agccccttgc ccgcaagatt tggaagcatt aaaggacctt
     8161 tgcgaacccc gtctatatcg cactcacctc gtttacacgc ttcataaatg tgaaccaagg
     8221 tgttagcacc cgcatctcca taccgagcgg catcaagact ggctccgatg ccaaaggaat
     8281 ccattactaa tacgcaaact cgtccggtca tactgtatgc tcaacttgtt ggcaacgagt
     8341 ttcttttaat ccgaacaata aaagtaatgc gatcaataat cctatgggta aaatgattgc
     8401 tgcatgctga tacgcttcga gtaaataaac tggcacacca ttaatcaaac gaacatcgcc
     8461 ataattcgtc aataaagcac taaacaggtt ttgataaata agatacccgc cttgagttaa
     8521 tatggacacg acactgacag ccgttgcagt catcatggga gggctgcttt cagcaaccag
     8581 ggcatagcta atgacttgag ctgaagtaaa taatcctaat aaaaagaaca atacggccat
     8641 cattgtcact gaaacaggta aataaagtac tcccagcaaa ataagtaaag aggcaaggac
     8701 tcctcctttc ataggcagca cacgcaaccc taccttatca gatatccacc ccatcaaagg
     8761 tgaacctata atggctccaa aaaacaacat accgttgatc atagctgcct gatcagggct
     8821 aatgcctaat ctctgttcaa gatataaagt ccccatcatg gcgccaaata cagcaatggc
     8881 catgttcata agactagtgt ataaggctgc tcgcaaattt tgagcattta agtaggcttt
     8941 tcttgcagcc acaagcacat tgataggttt tccttctttt ttgattgcga cttgaggtct
     9001 ttctttaatc caaaaaaaca tgagaactaa catggccaag cccaatatcc ctacgtcttg
     9061 aactgcttgg cgccatccaa catatagaac cagtttagtt aagggatatt gcgccagcat
     9121 tcctcctgtc attgcaaccg ttacaatcgc gccagtcact aaagccatac gctggggagg
     9181 aaaccaatga gaagccaaac gcacgggacc caaaaagcaa aatgcgctac ctattccggt
     9241 cacaaacctg caaaataaag ccacataaaa cgaatctgag taggagagga taaacgtact
     9301 aataacacac agaaacatag ctattaaaat ggtgtttttg attgaaaacc tgtccaaaac
     9361 cattccggca ataaacagaa aaatcacatt tgccaagtaa tagatgctgg ataagtaagc
     9421 cattttatct gcttgaatct gaaaatcctg catgatattt gctgcaatgg aagcaaacat
     9481 atttccctga ataaattcat aaaagaaaaa catggttgct gcaaaacaaa ccagccatgg
     9541 cattaacgat gctttaacat cgaatgcttt aaattcatcc actacctgca ttatttctcc
     9601 tctctatcgc ttgcagtatg tttcacgagt catgagcaca gcaaccagtc ctgcaattgc
     9661 ggcaacagga aacatccaca tggcaaattg aaaatcgcta accgtatatg ccgtaacatt
     9721 tgttcccgcg tgatgtgtca taagccaacc aaacagaact tgagcaacac cacccccacc
     9781 cataataatg acagaagcaa ttccagttgc agcaccagta ttttctagct gattgctttc
     9841 agcaaccagg gggtaagaaa tcacttgcgt gctggtaaat aatcccaaag caaaaaagag
     9901 gatactgaga ctcatttgag tcagaaccac attgataaat agaggaatag tagtaatcaa
     9961 agtagctatc gctccgaaaa tcattaaggg tttgcgacgt ccctgagtat cggataacca
    10021 accaaccaat gggcaaccga caacactccc catgaaaata aggctgacga cattgcttgc
    10081 cgcaatatca ggcaaatgat gagccacctg caggtaactt gcaccccata gagcgcacaa
    10141 aaccataatg ggtaaattaa gtagagaggt atataagcct gccagccaat tttgtttatt
    10201 ggatagagct ttcatgaaac tcgatagtat ttgtccttca ttggttaatt tatgtgctgg
    10261 tgactcttca ggcctgtctt gcacaatcat gtaaatccat aatattaaaa aagctccaac
    10321 aaccccatca attaacaatg cacgtcgcca gccaaacaga tcattcaaat aagcaaaagg
    10381 agtatgcgcc atcatgccac caataaatgc catagtaact aatgagccaa tgactaatgc
    10441 ctgtcttctg ggaggaaacc aatgagagac caaaacaaca caagataaaa agcaaaacgc
    10501 attgcctatt cccgagagaa aatggaaaaa tgaggcgaga aaaaatgatt ccgtaactgc
    10561 aaaccctatt gttccaacaa cacaaaccaa cattgcggtg agaataactt tcctggttga
    10621 aaacctgtcg agaataaggc cagctggtaa aagaaaaaga atatcagccc ataaataagt
    10681 gctcgacatc caacttaatt gggtggcatc tatatgaaaa tcttctcgaa gcgactgatt
    10741 aataacatca aaaatattga gttgaaaaaa ctcgtataaa aagaaaagcc ccgcggaaag
    10801 acacactacc caagccataa agtcaccacg gggaacaaaa gttctcgact ccactgcgac
    10861 agaatgcgac accacctctc ctttatttaa ggcctaaaaa ctgtggcaaa ttatatcaaa
    10921 atttaaaaag tattggtatc tttattgaac aatttatttc ttggaaaggt ctttcctgct
    10981 tcacctcgtt tataatgcaa ccgacaggta gcaacatagg attcattgcc gcctatcaat
    11041 acctgctcac cctcaataac agcttgccca ttttcatcaa ttctcatatt cataattgct
    11101 ttgcggccac aatgacaaat tgtctttaat tcgattaact catctgccca tgccagtaaa
    11161 aattgacttc ccggaaataa ctcgcctcta aagtcagtgc gcaacccata agctagtact
    11221 gggatagaca tttgatcggt tatttccgtt aattgataca cttgctctcg agttaaaaat
    11281 tgagcctcgt caatcaggac acaagagtat ttctgagttt gtagttgaaa ttcttgagtt
    11341 aaaacataca ggttatccga attgttaaaa gcataagcct gttcggatac accgatacgt
    11401 gaacagatag ttccatattg gaaacgtgta tcgatggctg gggtaaacaa cagagtttgc
    11461 ataccgcgtt ctctgtaatt ataactagac tgcagtaaca ccgtactttt acctgcattc
    11521 atagccgcat agtagaaata taattttgcc atagattgat tattt
//
LOCUS       DACTJE010000100        11432 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.100, whole genome shotgun sequence.
ACCESSION   DACTJE010000100 DACTJE010000000
VERSION     DACTJE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.100"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..821)
                     /gene="dapF"
                     /locus_tag="I9760_002002"
     CDS             complement(<1..821)
                     /gene="dapF"
                     /locus_tag="I9760_002002"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAT5043765.1"
                     /translation="MGIKFTKMHGLGNDFIVLDGVNQSIQLTVEQIQKLANRHTGIGF
                     DQCLLIESSQTEGIDFNYRIFNADGQEVGQCGNGARCIALFARYYGLTAKNKLTVATK
                     TTLMDLIINEDNSVSVNMGVPRLAPGEIPLLADRQSPEYSLELNNGNTVNLHAISVGN
                     PHAVLLVENIDTAPVNSLGQQISFHPQFPEQVNVGFMQIINHEKINLRVYERGCGETI
                     ACGSGAVAAAAIARLFYNLSDKITVHLPGGDLCIQWPCPTAPIILTGPAAFVYEGT"
     gene            complement(831..959)
                     /locus_tag="I9760_002003"
     CDS             complement(831..959)
                     /locus_tag="I9760_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopeptide precursor"
                     /protein_id="HAT5043766.1"
                     /translation="MRHIYFALLLIVVTLTSLCLSACGQKGPLYLPEQEKKTTTNQ"
     gene            complement(1118..1480)
                     /locus_tag="I9760_002004"
     CDS             complement(1118..1480)
                     /locus_tag="I9760_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043767.1"
                     /translation="MSILFLARKLASDCGFIEQIQQSHNRNQINYRFYSPRTDLHGSN
                     TNRLYGSPGKGVISQNTIFIYWYRDGKNSYFIECSNRLPKKELEVLHQLLMDNQHSLE
                     EILEASNNRFLSFQTRHT"
     gene            1670..2317
                     /locus_tag="I9760_002005"
     CDS             1670..2317
                     /locus_tag="I9760_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HAT5043768.1"
                     /translation="MKEPLEKAQACVIWMHGLGADASDMMGLADQLTIEDTALRHVFL
                     DAPRRPVTLNGGMVMPAWYDIYGLGFVDEEDKFGIEQSELLIRKVVDAQYNCGFKPHQ
                     IFLAGFSQGGAMALHTALHMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGAGQFD
                     PLVLPKWTLQSKDWLLANGYNEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ"
     gene            2314..2748
                     /locus_tag="I9760_002006"
     CDS             2314..2748
                     /locus_tag="I9760_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HAT5043769.1"
                     /translation="MTIVKRSRTVPYSCEQMYGLVNDVEHYSEFLPYCAESKILHRDN
                     DEVQATLVIAAAGMSKSFTTRNRLQTNKMIEIRLVDGPFSHLEGFWRFDQEEKGCRIS
                     FDLEFEFAGKIFSMLLGPIFDQITDKMVDAFCERAEVIYGKS"
     gene            2735..3007
                     /locus_tag="I9760_002007"
     CDS             2735..3007
                     /locus_tag="I9760_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAT5043770.1"
                     /translation="MVKVELVYIANSKNCLHYKMDLKQGATVEEALIESNIYSTCPET
                     RELPVGVYGKRVSVDQVLKEGDRIEIYRPLILDPKEKRRKLAQFKK"
     gene            complement(3096..3440)
                     /locus_tag="I9760_002008"
     CDS             complement(3096..3440)
                     /locus_tag="I9760_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAT5043771.1"
                     /translation="MAKFFMVGKKMRIMTFFICFILTLTLTQCASYDFSRRITQQGNL
                     LPQSKIARLKTGMSKNDVAILMGTSIMSPTFNNDRWDYAYTWRRGKGNLEIRNVVLYF
                     SRGSLVKIEHKP"
     gene            3638..4048
                     /gene="fur"
                     /locus_tag="I9760_002009"
     CDS             3638..4048
                     /gene="fur"
                     /locus_tag="I9760_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAT5043772.1"
                     /translation="MEESQQLKDAGLKITLPRIKVLQILEQSRNHHLSAEAVYKALLE
                     SGEDVGLATVYRVLTQFEAAGLVSRHNFEGGHSVFELSQGEHHDHLVCVKCGRVEEFV
                     DEIIEQRQKAIAERAHFKMTDHALNIYGICPQCQ"
     gene            4879..5511
                     /locus_tag="I9760_002010"
     CDS             4879..5511
                     /locus_tag="I9760_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT5043773.1"
                     /translation="MNFPIIAYNLYLFQFDKIIFAVMYSLFLIIVLLTVRLNSKLLSK
                     VRALSITDSLTGLFNRRHFDMVFSNELSRAKRNKYPISLVFIDIDNFKYINDTFGHSA
                     GDRFLIHVSNILKQTLRRSSDSMFRIGGDEYAAILINMPPNEVTTVCVSMQEQFNKKN
                     QYKNVSLSMGVISIDSMRVVDQQSAITAADKTLYQAKKAGKNQIKSKSLG"
     gene            complement(5824..6210)
                     /locus_tag="I9760_002011"
     CDS             complement(5824..6210)
                     /locus_tag="I9760_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043774.1"
                     /translation="MKKWTVLAASSLLMVNAYANESFCGYKDFFHLGDKTHPGIYVVS
                     GYNDSDVVLQIVGPRSFVIRDGFDCRAGYAHVTVAYDNANWCVLDINDGPFMNHPVVS
                     ASCNGLRYINTTYDGFGSYSYSINIE"
     gene            6466..7065
                     /locus_tag="I9760_002012"
     CDS             6466..7065
                     /locus_tag="I9760_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0375 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043775.1"
                     /translation="MYSSLFLKKDLGHLPEKVIFLDFDGTVALPARKENGEYDVQPDG
                     SIGATHLNKEKFRRLVQFSIHTDIPLYFLSGRPDLQSCVDLMTDFIASVDGFHSGIGG
                     FKKDSLFFISKMIIEEGKLIRKEITTKAQVIQSVHHEKYSYLPKESILLVDDIQEYLD
                     PVRELGYNTLLANPESLEHFNEVEEFIGVTLQKTIAFNT"
     gene            complement(7105..11394)
                     /gene="sdhA"
                     /locus_tag="I9760_002013"
     CDS             complement(7105..11394)
                     /gene="sdhA"
                     /locus_tag="I9760_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdhA"
                     /protein_id="HAT5043776.1"
                     /translation="MISEKIKLLESFKQNQFSKLIEKINSKILATLKSGHFNPRLAPY
                     LEKMNKDGVPFTNFDLDPENISQIKKLINALYHARLAFLDLENIDIGNIKRTIPDLKL
                     LYSNTIHQAYQAGYLITNLDVDLQEMFKEEIDLILPVIGKLQAFAGEHQECTKRLAES
                     WKDFPISYKAGEVTGIALEQMQPRTGDWDYQFLTQFSAVLPGYIEKLTQYIQQYSSQI
                     KEKEPTLNNEKLEELQNASLKLLNDLEHLKGNDFFISLKVLKYIHIIRNIITLATSSV
                     EQMGHFSDSSQDVIRDNLAQLKYVVLPTLFGLVDKIEDNAMLKPGTLSIPLMEKIKPL
                     YQLLIYYASKPVNFKEKGEELLSIEDSRFLALRLERTYQRIDEANKALFKIQKAQEAL
                     DNFYKILDTPPYNKQPIHQLPKEIKNQLITYYKIISPYMMDVDPDLNILLIDSFQGPE
                     SWSSYLKKPWRWLRGTLPADHASFVLAQKEALQTLISKKKATQQFHIELNRDLIESVH
                     KQTNLVLFPYSEKTNVFLLDETKALNPANGATDKLKFRPEKGHNILVNPEDLTSEQAL
                     DLYQWYRNKREKFRVASKAYNEFITLLKKQTKTIAETEGRILHLNNLDKETKTRCRNL
                     YNIFQPYFIDGIPPELRASAISFDKFLVHSFSNESSTYDAPAVDLFEKLDEHFQIYFT
                     EIDLQWGKKSNQYLKWAQEKFASENNSTELEHDEKLESRAHHLIKHTNFSKCIHEFRT
                     ELNQVISFFNNAMRAQLKQQPNGIPYPELQDHNKELAQSDQVVAIKRIFNSLYHVEKI
                     VVELEKLDTRSYESIYVYHLIQAYDHINEIKKLALKLAVDPHFKLIASELLEKAQTLI
                     ATIQEHSDAYQVSPLEVPYNQDIKYNALWYTLNAFYISPKHIRSLRNTNYLTTEELND
                     LHLKAKKATVTVENIIKSSNSYFKLFLQTPAMYSLYREMTNKLNEFISTSHDAVMNNL
                     DQFRSKIFTPMLMEADAWEDKLGLVPGTISGPLKTITDEYYKGLLHPLALHSKAHIRM
                     ICDKTPIDQRIKTTNKKLENATQHLDKIEKNYKHIIKLYEIIKTKGGKTTDDELIETY
                     KKALYKLVKLQKRLPQNTDPDPKDYELDTLLNSELKEYEPKLTLIKPLIISSYHHYEG
                     LKATYLMKANTAREKLTYLKELRSTQEQEDLLYIEEYTTESFNKQLDAFCNRHIGLQY
                     TDKEYRTKLREYLLTFKESIIQRSKSAEDINLTIKNLLKEKIKNFERDNFEKYYHLDA
                     VRVALAQFKNYFSLSTAAIEQNNSTFESEETLAKKTELINKLVDISEDGTLTPKERVD
                     QISFHVKNPNFERIIMDYKQESYFSFTYLKQCILSLLEALCLYTPERRKLFNNLESSV
                     KTQPKINELTKRFGLFAGNESAISSESRAITPVSSVENNGTIELNQLAPSA"
BASE COUNT         3366 a         1863 c         2265 g         3938 t
ORIGIN      
        1 gtaccctcat acacaaaagc agcaggtcca gttaatataa tgggagcagt gggacaaggc
       61 cattggatgc acaaatctcc tcctggcaaa tgaacggtga ttttgtctga caggttgtaa
      121 aataaacgtg ctattgctgc cgcagcaacc gcaccactac cacaagcaat cgtttcacca
      181 caaccccttt cgtagacacg taagttgatt ttttcatgat taataatttg cataaatcca
      241 acgttgactt gctctgggaa ttgcgggtgg aaactaatct gttgtcccaa actgtttaca
      301 ggggctgtat ctatattttc aaccaacaat acagcatgag gattgcctac acttatcgca
      361 tgaagattca ctgtattgcc gttatttaat tcaagtgaat attctgggga ttggcgatca
      421 gcaagtaaag gaatttcacc tggggctagt ctgggaacgc ccatattgac actcacgctg
      481 ttatcctcat tgatgattaa atccatgagc gtagttttag ttgctactgt caatttattt
      541 tttgctgtta aaccataata tctggcaaaa agagctatgc atcgcgcccc attaccgcat
      601 tgacctactt cttgcccatc ggcgttgaag attcgataat taaaatcaat tccttcggtt
      661 tgactggatt ctattagtaa acattggtca aaccctatac cggtatggcg attggctagt
      721 ttttgtattt gctcaacagt gagttgaata gattggttga ctccatccaa tacaatgaag
      781 tcattgccta aaccatgcat tttggtaaat tttattccca taggatgtct ctattgattg
      841 gttgttgttt tcttttcttg ttcaggcaaa tacaaaggtc ctttttgccc acaagctgac
      901 aaacacaaag aagtcagggt gacaacgatg agtaataatg cgaaataaat gtgtctcata
      961 gggtattatt atagagttaa attttaatta tatgagattt gctttgaaac tcaatgattc
     1021 attcttcttg ttattttata tgtgaatatt ttgagcttga gtaagacagg cagatagctt
     1081 attccgtatt ttgtgtcttt ctttagtgaa gtagtgctta ggtatggcgt gtctgaaaag
     1141 agagaaatct attattggat gcctcgagta tctcttccaa agagtgttga ttatccatga
     1201 gcaattgatg caacacttca agttcctttt taggtagtct atttgaacat tcaataaaat
     1261 aactgttttt tccatctctg taccagtata tgaaaatcgt attttgtgaa atgacacctt
     1321 tgccaggact tccatataac ctgtttgtat tggaaccatg cagatcagtt cggggggaat
     1381 aaaagcgata atttatttgg ttgcgattat ggctttgctg gatttgctca atgaacccgc
     1441 agtcagaagc cagttttctt gctaaaaaca aaatactcat aaaacaggac aacatacaaa
     1501 ataaacataa tgataaagat ttatacaata aagtcaatag gtattagaat taccagcgtc
     1561 aatatgccta taaattgttc aatatatttt taaatgttaa acttgcgctg ctctattgca
     1621 ttggcgataa tttacgtttt tgcgtggaga atttgttttg agcgtttata tgaaggaacc
     1681 tttagagaaa gcacaagctt gtgtcatatg gatgcatggg ttgggggctg atgcctctga
     1741 tatgatgggt ttagctgatc agctaacaat agaagatacc gctttaaggc atgtatttct
     1801 tgatgctcct cgaagaccag tgactctgaa tggaggaatg gttatgcctg cgtggtatga
     1861 tatttatgga ttaggctttg tcgatgaaga agataagttt ggcattgagc aatctgaatt
     1921 gttaatacgc aaggtggttg acgctcaata taattgtggc tttaaaccgc atcaaatctt
     1981 tttagctggc ttttctcaag ggggagctat ggcattgcac acggctcttc atatgaccga
     2041 gcgattatgc ggcgttattg cattgtccgc ttatttgccc ttggctaagc ataataagcc
     2101 gcaacttgat aagaatactc ccatctttat gggagcagga caatttgatc ctttagttct
     2161 gccaaaatgg actctacaga gtaaggattg gctcttggct aatgggtata atgaagtctc
     2221 ttttcatcaa taccctatgg aacattccat atgttttgaa gaaattaaag atcttagcct
     2281 ttggcttaat aagcaggttc aaggagtttt acaatgacaa tagtaaaaag gtcaagaaca
     2341 gtaccttatt cttgtgagca aatgtatgga ctggttaatg atgttgaaca ttattccgag
     2401 tttctacctt attgtgcgga aagcaaaata cttcatcgtg acaatgatga agtccaggcg
     2461 actttagtga tagcagctgc tggaatgagt aagtcattca ctactcgtaa tcgattgcaa
     2521 actaacaaaa tgattgaaat tcgtctggtt gatggtcctt ttagtcattt agagggattt
     2581 tggcgttttg atcaggaaga aaaagggtgc agaatttctt ttgatttgga gtttgagttt
     2641 gccgggaaaa tattttccat gcttcttggt cctatatttg atcagataac cgataaaatg
     2701 gttgacgcat tttgcgaaag agccgaggtc atttatggta aaagttgaat tagtctatat
     2761 agcgaattct aaaaattgcc tgcattacaa aatggattta aaacagggag ctacagttga
     2821 agaggcactc attgaatcaa atatttattc aacttgtcct gaaacgagag aattacccgt
     2881 aggagtatac gggaagcggg tttctgtgga ccaggtattg aaagaaggag atcgaattga
     2941 gatttatagg ccattgatac tcgaccccaa agaaaaacgc cgtaaattag cacagtttaa
     3001 aaaatagagg caaattaggc attttaatct acactgaact cttaaaaaac aattttcccc
     3061 ttgtctaatc acttaggcaa gggggagata ttaaattacg gcttatgttc tatttttact
     3121 aaagaacctc tggaaaaata taacacaaca ttacgaattt ccaggttacc tttgcctcgt
     3181 cgccaggtat aagcataatc ccacctatca ttgttgaatg ttggactcat tatgctggta
     3241 cccattaaga ttgcaacgtc attcttactc atgcctgttt ttaaccgggc aattttggat
     3301 tgaggcaaca aattaccttg ctgagtaatg cgtcttgaga agtcatatga tgcacattgg
     3361 gttaaggtta atgttaaaat gaaacaaata aaaaaagtca ttattcgcat ttttttgcct
     3421 accatgaaaa atttcgccat aataacatgt catttattga aaggcacctg tcatcatgtg
     3481 gaatttggta tttgccatta ttcggccaga ctaagtttta aggccgctaa tggtttttag
     3541 agcatgaaaa ttattttagg ccttgatgct aattattaat ccaattttgc tggaataatg
     3601 atcaaagctc gaaattcgtt acagttaagg agcacaagtg gaagagagtc aacagttaaa
     3661 agacgctgga ttaaaaatca cattacctcg tatcaaggta ttgcaaatat tggagcaatc
     3721 aagaaaccat catttgagtg cagaagcagt ctataaagcc ttattggaat cgggtgagga
     3781 tgttggttta gctactgtgt atagagttct tactcaattt gaagcagcgg gattagtatc
     3841 gagacataat tttgaaggag gacactcggt gtttgagttg tctcagggcg aacatcatga
     3901 ccatcttgtt tgtgttaagt gtgggcgggt agaggaattt gtagatgaaa taatagaaca
     3961 aagacaaaaa gcaattgccg aacgtgctca ttttaaaatg acagatcatg cgctaaacat
     4021 atatggtata tgtccgcagt gtcaatgact ttgatatttt aactggattg gcctgtcacc
     4081 tcacggaata ttataagcgt atttactata ttcttttgca gtgctttatc attacaataa
     4141 tcagagtgtt ttggtaaaac gttattttct ttaaggattc atcacatgga gtttgattat
     4201 attattgtta gtgatggtag cccaggatag gcttttgctt ccaggttaag tgccgatccc
     4261 atcaataaat tattgttgct ggaagcggaa gatagcctat ttctggaaat gccaatccgc
     4321 ttgcaatgag aatcgcagaa caattatcac actatattct aattcttcgt gagagcctcc
     4381 tattctaaat ggaatttggg cggtaaagag taacactgta aactagtcta tacttgaaat
     4441 tataatgaag ttgcttgggt tttgaattaa ttatgcatca aaagaagcgg tcttcattgg
     4501 aacttgaaga cttgttgtag ttatttgagg gatccttaag aactattcca ttcaatatcc
     4561 tggcggtgat gtgcttgctt tgggttttat ctatacgaac gagattccaa tcaaactggt
     4621 tattatctgg tttttctcat tttaatttta agtgtgattc ggtgggtaac tagtcgaatt
     4681 ataatcagta aagagtttta catcacaaaa ggaaagcagt ctttaattgg ttttttactg
     4741 ctggattttt ttatgggatt ggcttggagt ttgagctact ttattttttc cccttattta
     4801 aatgacacca gtgagtttat ttttgtattc atttcttaat caatttatct tcccgcctat
     4861 tatgcttacg tattgccaat gaatttccca attattgcct acaatttgta tttatttcag
     4921 tttgataaaa taatttttgc tgtcatgtat tcgctttttt tgatcatagt attgttgaca
     4981 gtaagattaa actcaaaatt actttccaaa gtgagggcat tatctataac cgattcattg
     5041 actggattat ttaaccgtcg tcattttgat atggtgttca gcaatgaact aagcagagcg
     5101 aaacgtaata aatatcctat cagcctggtt ttcattgata tagataattt caagtatatt
     5161 aatgatactt ttggtcattc tgcaggtgac aggtttttaa ttcatgtgtc aaatatcctg
     5221 aaacaaacgt tacggcgctc aagcgatagc atgtttcgga ttggtggcga tgaatatgct
     5281 gccattctta ttaatatgcc accaaatgaa gtaacaacag tttgtgtttc aatgcaagag
     5341 cagtttaata aaaaaaatca atataaaaat gtgtcgttaa gtatgggggt cataagcatc
     5401 gactctatga gggtagttga tcaacaaagt gccattactg cagcggataa aactctttat
     5461 caagcaaaaa aagcagggaa aaatcaaatt aaatctaaat cgttaggcta gcaagaattg
     5521 agagtcaatt aaaattgttg gatttgatta cagcaagtat aagataagag aatacaattt
     5581 tatttctctt atgtcagaga tggcctctgg attaattact tttaacgtga gttatggata
     5641 agatgctcgt tatcttggat tgtttgagcc gagggggcat ttatgatgcc tcgcgaagcc
     5701 ttgtatgaaa tttaatgatt cttgccgggg cttcgagaag atgctcatgt atcttctcag
     5761 cccaaacggt ccctggatat cgattgcttt ttatataatc gctatccata actcacgtta
     5821 cttttattca atgttgatag aataggagta acttccaaag ccatcatagg tggtattgat
     5881 gtaacgaaga ccattgcagg aagcactgac aacaggatga ttcataaatg gaccgtcatt
     5941 aatatcaagt acgcaccaat tggcattgtc ataagctaca gtgacatgag catagcctgc
     6001 tcgacaatca aaaccatctc tgattacaaa gctacgtggt ccaacgattt gtaatacgac
     6061 atctgagtca ttataaccac tgaccacata aattccaggg tgagttttgt cacctaaatg
     6121 aaaaaaatcc ttgtacccac agaatgattc attggcgtag gcgttgacca ttaaaaggct
     6181 acttgctgcc agtactgtcc attttttcat acgaaaatac cttcttattg ttattcagga
     6241 aatcgaatta tagggaatcg atggctatta aacaatcatc ttgttctttt gtttttgaaa
     6301 aacctgttct gcaagtgatt tctggaaagg gtaggaaata tttcctgtta aagagatgtt
     6361 cagttacctt gtttcgattg atctaatcgg atacgctgac ctgtagagat tgagaatctc
     6421 cacaatattt tgaagggata gaaacattaa gtatcaggag gaaaaatgta ttcatcatta
     6481 tttctaaaga aggacttggg gcatttgcct gaaaaagtta tttttcttga ttttgacggc
     6541 accgttgctc ttcctgcaag gaaggaaaat ggagaatatg atgttcagcc tgatgggtca
     6601 attggagcta cccacctaaa taaagaaaaa tttagacgtt tagtacagtt ttctattcac
     6661 actgatattc ctttgtactt tttgagcgga cgtcctgatc ttcaatcttg tgtcgattta
     6721 atgactgatt ttattgcaag cgttgatggc ttccattctg gtattggcgg gtttaaaaag
     6781 gattctctgt tttttatcag taaaatgata attgaagagg ggaaacttat tcgaaaggaa
     6841 ataacgacaa aggctcaggt gattcaatcc gtgcatcatg aaaaatacag ttatttacct
     6901 aaggaatcga ttttactggt cgatgacatt caagaatacc tcgacccagt gcgggaactg
     6961 ggttataaca ctttgttggc aaatccggag agtttggagc attttaatga agtagaggaa
     7021 tttattgggg taacattaca aaaaactatc gcattcaaca cctagacaag gttatggtaa
     7081 attaaaagat taaatcaaat atttttatgc ggatggcgct aattggttta actcaattgt
     7141 tccattattt tcaacagaag aaactggggt aattgccctg gattcactag agatagctga
     7201 ctcattgcca gcaaataatc caaatcgttt ggtcaattca ttgattttag gctgggtttt
     7261 tacagagctt tccaaattat taaatagttt tcttctctcc ggagtgtaca ggcaaagcgc
     7321 ttccaataag gataagatgc attgttttaa ataggtaaag ctaaaatagc tttcctgttt
     7381 gtaatccatg atgattcttt caaaattagg atttttaaca tggaatgata tttggtctac
     7441 acgctctttt ggcgtaaggg ttccatcttc actgatatca accagtttat taatgagctc
     7501 tgtctttttc gccagtgtct cttcgctttc aaatgtcgaa ttattttgct cgatggcagc
     7561 ggtagaaaga ctaaagtagt ttttaaattg agctaaagcg actcttactg catcaagatg
     7621 ataatatttc tcaaaattat ctctttcaaa atttttaatt ttttccttta gcaaattttt
     7681 tatagttaaa ttaatatctt ctgcggattt tgagcgttga ataatacttt ctttgaaagt
     7741 cagtaaatat tctctaagtt ttgtgcgata ttccttgtca gtgtattgta aacctatatg
     7801 acggttgcag aaagcatcta attgtttatt aaatgattct gtagtgtatt cttctatata
     7861 aaggagatct tcttgttcct gagtcgatcg taactcttta agataagtca acttttctct
     7921 ggcagtattt gctttcataa gataagtggc ttttaagcct tcataatgat ggtagcttga
     7981 gataatgaga ggcttaatga gcgttaattt aggttcatac tctttcagct ctgaatttaa
     8041 aagagtgtct aattcataat ctttaggatc aggatctgta ttctggggta atctcttttg
     8101 tagttttacc aatttgtaca gagctttttt ataagtttcg attaattcat catcagtggt
     8161 tttgccacct tttgttttga taatttcata tagcttgatg atatgtttat aatttttttc
     8221 tattttatcc aaatgttgtg ttgcattttc gagtttttta ttcgttgttt ttattctttg
     8281 atctattggt gttttatcac atatcattcg aatatgtgct ttggaatgca gcgctaaagg
     8341 atgtaacagg cctttataat attcatccgt tattgttttt aatggtcctg agatagtgcc
     8401 tggtaccaat cctaatttat cttcccatgc gtccgcttcc atcagcatgg gtgtaaaaat
     8461 cttggagcgg aattgatcca gattattcat tacggcgtca tgtgatgtgc taataaactc
     8521 attcagtttg ttcgtcattt ctctatagag agaatacatg gcaggcgttt gtaagaataa
     8581 tttgaaatag gagtttgaac ttttaatgat attttctaca gtaacggttg ctttttttgc
     8641 ctttaagtgt aagtcgttga gttcttctgt cgtgagatag ttggtatttc ttaaacttct
     8701 aatgtgtttt ggcgaaatat aaaaagcatt gagtgtatac cagagagcat tgtatttaat
     8761 atcctggtta taaggtactt ctaaggggga tacctgatac gcatcactgt gttcttgtat
     8821 tgttgcaata agagtttgcg ctttttccag taactcgctt gctatgagct taaaatgagg
     8881 atcaacagcg agttttagcg ctaatttctt aatttcatta atgtgatcat atgcttgtat
     8941 taaatgatag acataaatgc tttcgtaact tctggtgtcg agtttttcca gttcaacaac
     9001 aatcttttca acatgataga ggctattgaa tatgcgtttt atcgcaacaa cctgatcact
     9061 ctgggcaagt tctttgttgt gatcttgcaa ttcagggtaa ggtattccat tgggttgttg
     9121 ttttaattgt gccctcatcg cattattaaa aaacgaaatg acttgattaa gctccgttct
     9181 gaactcatgt atgcatttag agaaattggt atgtttaatt aaatgatggg ctctgctttc
     9241 cagtttttca tcatgttcaa gttcggttga gttattttca ctggcaaatt tttcctgggc
     9301 ccattttaaa tattgattac tctttttacc ccattgtaaa tcaatttctg taaaataaat
     9361 ttgaaaatgt tcatccagtt tttcaaacag gtcaactgcc ggagcatcat aggtggatga
     9421 ctcattagaa aaggaatgga ccaaaaattt gtcaaaactt attgcggaag ctctgagttc
     9481 aggaggtatc ccatcgataa aataaggttg aaatatatta tataaattac ggcagcgagt
     9541 tttggtctct ttatcaagat tatttaaatg gagaatcctg ccttccgttt cagcgattgt
     9601 cttggtttgc ttttttaata aggtaataaa ttcgttatat gctttgctgg caactctgaa
     9661 tttttcacgt ttgtttctat accattgata gaggtcaaga gcctgttcag atgttaaatc
     9721 ctcaggatta accagtatat tatgtccttt ctctggcctg aattttagtt tgtccgtagc
     9781 gccattagca gggtttagtg cttttgtctc atctagaaga aagacattcg ttttttcact
     9841 ataaggaaat aaaaccagat ttgtttgttt gtgaacagat tcgataaggt ctctattcaa
     9901 ttcgatatga aattgttgag tggctttctt tttgctaatt aaagtttgta atgcttcctt
     9961 ttgcgctaaa acaaaactgg cgtgatcggc aggaagagtg cctcttaacc aacgccacgg
    10021 cttctttaaa taagaagacc aggattccgg gccctgaaaa ctatcgatca acaaaatatt
    10081 gagatcagga tccacatcca tcatataagg actgattatt ttgtaatatg taatcagttg
    10141 gtttttaatt tctttcggta attgatgtat gggttgctta ttataggggg gggtgtccaa
    10201 tattttgtaa aaattatcca gagcttcttg cgctttttga attttaaaaa gggctttgtt
    10261 agcttcatca attcgttgat atgttctttc cagtcttaaa gcgagaaaac gggagtcctc
    10321 tatactgagt aactcttctc ctttttcttt gaaattgacg ggtttggatg cgtaataaat
    10381 taaaagttgg tatagaggtt tgattttttc cattaacgga atggacagtg tgccaggctt
    10441 cagcatggca ttgtcttcaa ttttatctac taatccaaat aaagtaggta atacaacgta
    10501 ttttaattga gccaaattat cacgaatgac atcctgggat gaatcgctga aatgtcccat
    10561 ttgttcaaca gagcttgtcg ccaatgtgat gatattacgg ataatgtgaa tgtattttaa
    10621 aactttaagg gatataaaaa agtcattccc tttcagatgt tccaggtcat ttagtaattt
    10681 taatgatgcg ttttgtaatt cttccagctt ttcattattt agggtaggct ctttttcttt
    10741 gatttgcgaa gagtattgtt gaatgtattg agttaatttt tctatataac caggtaatac
    10801 ggcactgaat tgagtgagga attggtaatc ccaatctcct gttctcggtt gcatttgctc
    10861 taaggcaatg cctgtaactt ctcctgcttt atagcttata ggaaaatcct tccaagactc
    10921 tgccaaacgc ttggtgcatt cctggtgttc accagcaaaa gcttgtaatt ttccaatgac
    10981 agggagaata agatcaatct cttccttgaa catttcctgc aaatcaacat cgaggttagt
    11041 gattagataa ccggcttgat aggcctggtg aatcgtgttg ctataaagta atttgagatc
    11101 ggggatagtt cgtttgatgt tccctatgtc aatattctct aaatccagaa aagctaaacg
    11161 ggcatggtaa agggcgttaa tgagtttctt gatttgagaa atattttccg gatcaagatc
    11221 aaaattggtg aatggaaccc cgtctttatt cattttttcc aagtaggggg ccagtctggg
    11281 gttgaaatgt ccacttttta aagtcgcaag gatttttgaa ttaatttttt caattaattt
    11341 agagaattgg ttttgcttaa aggattctaa aagcttgatc ttttctgaaa tcatttcaat
    11401 attaaaaaaa ttaacttgtg atattataaa ga
//
LOCUS       DACTJE010000101        11272 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.101, whole genome shotgun sequence.
ACCESSION   DACTJE010000101 DACTJE010000000
VERSION     DACTJE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11272
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.101"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1714)
                     /locus_tag="I9760_002014"
     CDS             complement(<1..1714)
                     /locus_tag="I9760_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT5043777.1"
                     /translation="MLTDAQIQHAINNLNILIAHYNSPISCFSFDNERVTRNWFFKSE
                     STHATALSNYLKKIIDEVSSDKVDKTNSLYSSLFISLQNFLHLQPNRVIEGLNFNLEN
                     SQYLKRKNLIIKRLEHFHIENQFRDDLKRHLLPEIQYDTCTGHYTSPDDDKAHQHGSE
                     AQRIFFSTQWERLKALCNWLLAKMGIHIFETARYKEHDYLDSEVYANDPAIEPGVSPQ
                     QSATHYWIGHASNLMTIPAHDSPLHVVTDPVEGDLAPFLYPRMTKEGSLIDGTGSKKL
                     PKIDIVVISHNHHDHVSQSTLKRLLKQQPKMVVPEGDESFFLGMGFTDVVGLKWWEQA
                     KITDFQGNELLRITAVPARHWSGRSLTDAHCSAFNGYVLHANQLDGDIYFAGDTALMD
                     DMLSQPIFEKFNIVTSIQPGGPDERREDMESTHQSSADAILMHFKILAAQYQKMKEKN
                     ESLSLDDFLIENQHLKTLYNHTATFKLGNLRLKDTYYSYQRMVAAFREDAQWRADHLP
                     KHEQKVYDHIQLLISNMVFKDNQQLSNRQIADLILGKVVIPKIGQRQPLYFKEKPIKT
                     ETFQY"
     gene            complement(1806..3698)
                     /gene="ravS"
                     /locus_tag="I9760_002015"
     CDS             complement(1806..3698)
                     /gene="ravS"
                     /locus_tag="I9760_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavS"
                     /protein_id="HAT5043778.1"
                     /translation="MCIEKILEPVIMSGIKKFNLTYQQVPKIFSLPESVFLSKDVELI
                     PEEVLKQTKTQKNIVAKTIEQIDKDLERTCYVDFDGLTVAQLKSDWLTVNPQGDLALS
                     EGVYPSFSQGDNTGAVIGEILQTKHFSLPLSEEDPKAFMEVTRNTTRICFSYKNSAGQ
                     PSGFSLVAAPNGDSEGNHKGWIISVIQNTVGNYKGRKVSVLASPNYVRSEAQSTVDMD
                     TPDAIERELKRVINNDKVASLIKGIFKEDGTLNADYIRQLASRIKGNRNIDDRDRKSA
                     QFNILIELAEQLDDEKLRHYINSIKADMAADIEFFKDGIYEQHLSELSNKISLSLSKM
                     PSVNKKTTEIGRILNNISERYAQIKMIEDAQRKNPELSHIFKAMAQKIKSDFHDIKFD
                     GTHHDNRNILLLSQFEVASVEQKIRNYYQTNPLVKQYFVDKDKANHHLQVELSRLSPP
                     KNEGFFSRNKDSFGFSIFMGLIVGVSAALFLTGVLAPIGVALIAVGAALITTGLVGSI
                     DIYVNEKEYANETSHYSHEVKCLRSAHREHLVRLEKNLHEQINLSDFLTPDKPEFLSG
                     IKVNKKKPLTPERVAHALWLQDKSARDTSVAKNRFSIWAAKQENLDFGIEEVESVIKQ
                     RYVMKS"
     gene            complement(3871..4695)
                     /gene="kdsA"
                     /locus_tag="I9760_002016"
     CDS             complement(3871..4695)
                     /gene="kdsA"
                     /locus_tag="I9760_002016"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAT5043779.1"
                     /translation="MRLCGFEAGLDKPLFLIAGPCVIESEELALETAGYLKEMCSQLN
                     IPFIYKSSFDKANRSSISSYRGPGFEKGLSILEKVKSQIGVPVLTDVHEDTPLFEVSS
                     VVDVLQTPAFLCRQTNFIQKVAAMNKPVNIKKGQFLAPWEMKHVIAKAKAQGNEQIMA
                     CERGVSFGYNNLVSDMRSLVIMRETGCPVVYDATHSVQLPGGNNGVSGGQREFIPALA
                     RAAVAVGISGLFMETHPDPDKALSDGPNSWPLDKMKQLLESLKAADEVYKKYSTDF"
     gene            complement(4692..6329)
                     /locus_tag="I9760_002017"
     CDS             complement(4692..6329)
                     /locus_tag="I9760_002017"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAT5043780.1"
                     /translation="MTKYIFITGGVVSSLGKGIAAASLAAILEARGLRVTLIKLDPYI
                     NVDPGTMSPFQHGEVFVTNDGAETDLDLGHYERFVKTTMTKRNNFTSGKIYENVIKKE
                     RRGDYLGGTVQVIPHITNEIKRCIKLGADAFDVAMVEIGGTVGDIESLPFLEAIRQMR
                     IELGSQRAIFIHLTLVPYIATSGETKTKPTQHSVKELRSIGIQPDVLICRSEKPLSMA
                     DRAKIALFTNVEKEAVISLEDANSIYQIPMILHAQHLDEIVVKKLSLEAKQADLSEWQ
                     RVVDMQAVQTMTVKIAMVGKYTELNDAYKSINEALLHAGIHTETKVEIIYFDAEMIEK
                     HGALLLESIDAILVPGGFGERGVEGKIKAIQYAREHKVPFLGICLGMQTAVIEFARNV
                     VGLTGANSTEFNKETLYPVLGLISEWMDADGSKQIRDENTDLGGTMRLGGQYCHLAEG
                     TLARKVYGKPQIIERHRHRYEVNNKYVDSLVKHGLIISGRSADNSLVEMIELADHPWF
                     LACQFHPEFTSNPRDSHPLFKEFVLAARIHHQEKDKK"
     gene            complement(6455..6922)
                     /locus_tag="I9760_002018"
     CDS             complement(6455..6922)
                     /locus_tag="I9760_002018"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAT5043781.1"
                     /translation="MREIKTIVSDGVTFPIALVVSTFNELITSALKKGALDRLTELGF
                     KSEDITLVEVPGAVEIPFVAQLLAKKQKVEVIVALGAVIRGETSHYDYVCDQVSQGCQ
                     RVMLDYNIPVIFGVLTTENDEQALARVGGTHGHKGRDAIDCAVSMRSIKQQLQ"
     gene            complement(6930..8138)
                     /locus_tag="I9760_002019"
     CDS             complement(6930..8138)
                     /locus_tag="I9760_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HAT5043782.1"
                     /translation="MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATP
                     EAINFMSRLGCGLICLPMAEELIDKLQLPMMARHNKSPYGTAFTVSIEAANGVSTGIS
                     ARDRARTIQVAIDPKSGPSDIISPGHVFPLRARKRGVLERPGQTEGSVDLVKLAGLTP
                     AAVICEIINEDGTMSRRDELALFSKKHQIPLVTIKDLIQYRVRHENLIHPVATTQIPL
                     EENGVFTMTVFENQIDSCEHFVLVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQ
                     LELSLSQIGAEGGILIYLRQEGRGIGLANKLKAYALQEQGFDTVDANLELGLPADDRD
                     YAVAYQILKYLGVEALRLLTNNPLKIASLERYGMKITQRIPLEIEPSRENHSYLKTKK
                     IKLGHLLAID"
     gene            complement(8135..8749)
                     /locus_tag="I9760_002020"
     CDS             complement(8135..8749)
                     /locus_tag="I9760_002020"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAT5043783.1"
                     /translation="MFTGLIENTGKVLMNRQGENSSRLVVSSSYQDLKPGESIAVNGV
                     CLTLLPADFPELCFDISPETLHVTTLSKLSSGDMVNLERAMLASTRFGGHYVSGHVDT
                     RAKVKSIRAVNEFIAMDLDGFIPEHNHYLIPKGSITVDGVSLTINTVSEQTVGLMLVP
                     HTLSMTNLGSLRVGQQVNIEFDYLTRIVAHQLQIIGKLNLEEVS"
     gene            complement(8734..9807)
                     /gene="ribD"
                     /locus_tag="I9760_002021"
     CDS             complement(8734..9807)
                     /gene="ribD"
                     /locus_tag="I9760_002021"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAT5043784.1"
                     /translation="MHEQFLLAALEQAKLGRGFCAPNPSVGAVAVQNGNIIAQAWHRG
                     AGTPHAEQLLLSQIPPQTPGVTLYVTLEPCNHWGKTPPCVDAIINHGIEEVVFSYFDP
                     NPIVAKSNSSEKLREHGIQVRHIPVKAIDVFYKSYAYWTMTHKPRVTVKIAQTFDGKI
                     GKSEGARVVLSNALCEQFTHQMRAASDVILTSAKTVQMDNPRMNARLDSGLVAKPVAI
                     VDRNLSLSDDTIIFSTAKHCHIYHQPQANQAKTYPNSTHYAMPLQNGLMDLEAIITHL
                     GQLGYHDVWVEAGGAIFSALHSKGLVHRTYLYLVPGSLGHQAISAYQQQGIFEKAHTV
                     SWQAMGDNMIACLDWQEDECLPD"
     gene            9870..>11272
                     /locus_tag="I9760_002022"
     CDS             9870..>11272
                     /locus_tag="I9760_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HAT5043785.1"
                     /translation="MIKLNLNYKLITKKTLPKLFYWGLGIVFFFLAPTTNAGSLSPYT
                     TKELEQLEREFIQLINQSDSVERNPLASQYINSIGKKLAHYGRIKTPYFFIVKSKEIN
                     AFAGPGGYIGINTQLILTSSNESELAAVMAHEIAHVRLHHLYSIIEHQKQMRVPLLAS
                     ILASAALGVLNPTVGMGAMMASVSGFSQDNINFTRSKEKEADRIGIDMLIKAGFNPQG
                     MAAFFNKMQENSRYYYTANIPAILRTHPLDADRIAEAENRTSRLPKKHYTDSLDYALF
                     KELIRVSVASNNKQILDYYEHHCHKKNPATACQYGYVLALLNNNNFQKAAEKLNPLLL
                     QDNSNLNFQIAMAQAELGLSQYQSALSRLAELQNNFPDNYAALMAYAQGLLTANQPQK
                     ATAVLLKGSRQYKEDLPLCRELARAQAESHQKSYAYFTQAQCLLLEGQKRGAIAQLKV
                     AQKLAKRDAYLSARISA"
BASE COUNT         3374 a         2425 c         1945 g         3528 t
ORIGIN      
        1 gatattgaaa tgtttctgtc tttatcggtt tttctttaaa ataaagtggt tgtctttgac
       61 caattttggg aataactacc ttgcccagaa tcagatcggc aatttgccga ttactaagct
      121 gttgattatc tttgaaaacc atattgctta tcaaaagttg aatatgatcg taaacctttt
      181 gctcatgctt tggtaaatga tcagctctcc attgagcatc ttccctaaat gcagcaacca
      241 ttctctgata actgtaatag gtatctttca gacgtaaatt tcctaatttg aaagtagcag
      301 tatggttata aagagtttta agatgttgat tttcaattaa aaaatcatca agagataaac
      361 tctcattctt ctctttcatt ttttgatatt gtgcagctaa tattttaaaa tgcatcagaa
      421 ttgcatccgc agaagattga tgagtagact ccatgtcttc acgccgctca tctggaccac
      481 cgggttgaat agaggtaacg atattgaatt tttcaaaaat gggctgtgat aacatgtcat
      541 ccattaacgc ggtatcccca gcaaaataaa tatctccatc taattgattt gcatgcagca
      601 catagccatt aaatgctgag caatgagcat cggtcagcga tcgacccgac caatgcctgg
      661 caggcactgc agttatcctt aataattcat tcccttggaa atctgtaatc tttgcttgtt
      721 cccaccattt caaccctaca acatcggtaa atcccatgcc taaaaagaat gattcgtcac
      781 cttccggaac taccatcttg ggttgctgtt ttaataggcg cttcaatgtg gactgactta
      841 catgatcatg gtgattgtga gaaatcacta ctatatcaat tttgggtaat tttttactgc
      901 cagttccatc aattaaactc ccttcttttg tcatacgcgg gtatagaaat ggagccaaat
      961 caccttcaac aggatcagtg actacatgca atggcgaatc atgggccggt atggtcatta
     1021 gatttgatgc atggccaatc cagtaatgag tggctgattg ttgtgggctt acccctggtt
     1081 caatcgcggg atcattggca tagacctcac tatccaaata gtcatgttct ttataacgtg
     1141 cggtttcaaa aatatgaata cccattttag ccaataacca attgcataag gcttttaatc
     1201 tttcccattg agtagagaag aaaattcgct gggcttctga gccatgttga tgcgctttat
     1261 catcgtctgg cgaggtataa tgcccagtgc acgtatcata ttgaatctca ggaagaagat
     1321 gtcgtttcag atcatccctg aattgattct cgatatgaaa atgttccagt ctcttaataa
     1381 tcaagttttt gcgtttcagg tactgactat tctctaaatt aaaattcaag ccttcaatta
     1441 cccgattcgg ttgtaaatga aggaaatttt gtaagctaat aaacaaacta gaatataaac
     1501 tattagtctt atctactttg tcgcttgaaa cttcatcgat aattttcttt aaataattac
     1561 tcagggcagt agcatgtgtt gattcagatt tgaaaaacca gttccttgta actcgctcat
     1621 tgtcaaaaga aaagcaggat attggcgagt tatagtgtgc aataagtata ttaagattat
     1681 taatagcgtg ttgaatttga gcatcagtta acatattttt ttaccgtatt atccaatcag
     1741 ctgattcgtt tgctatattt tgccaagagt tataaatgtc tgcttcattg aattctcaat
     1801 attttttatg atttcatgac atatctttgt ttgattacgc tttccacttc ctctatacca
     1861 aaatccaggt tttcctgctt ggcagcccat atcgaaaatc tattttttgc tactgaagta
     1921 tctcgagcac ttttatcctg tagccataaa gcatgggcca ctctctccgg agtcagcggt
     1981 tttttcttat tgactttgat gcctgataaa aactccggct tatcaggagt tagaaaatca
     2041 gaaaggttga tttgctcatg taaatttttt tctaaacgaa ccaaatgctc cctatgagca
     2101 ctccttaagc attttacctc atgagaataa tgcgatgttt cgttagcgta ctctttctca
     2161 ttgacataaa tatcgattga ccctaccagt cctgttgtaa tcaaggctgc tccaacagca
     2221 atgagagcta ctccgatggg ggcaagaacg ccagtcaaaa ataatgccgc actgactcct
     2281 acgattagac ccataaaaat actaaatcca aatgaatctt tatttctact gaaaaaaccc
     2341 tcatttttgg gaggagataa ccggcttagc tcaacttgta aatgatgatt tgctttatct
     2401 ttatctacaa aatattgttt taccagagga ttggtttgat aatagtttct aatcttctgt
     2461 tcaacacttg cgacttcaaa ttgacttaag agcaaaatat ttcgattatc atgatgtgtt
     2521 ccatcaaatt ttatatcatg gaaatcggat tttatttttt gagccattgc cttaaaaata
     2581 tgggataact ccgggttctt tctctgtgca tcctcaatca ttttaatctg tgcataacgc
     2641 tcagaaatat tatttaatat tctgcctatt tcagtagttt ttttatttac agaaggcatc
     2701 ttacttaacg ataaggaaat cttgttggat aactcactta gatgctgctc ataaattcca
     2761 tctttgaaaa actcaatatc tgcagccata tcagctttta ttgaattgat atagtgtctt
     2821 aatttttcat catccaattg ttctgccaat tcgatgagaa tattgaattg tgcagatttt
     2881 cggtcacgat cgtctatatt tcgattccct ttgatccgtg aagccaattg tcgaatataa
     2941 tccgcattga gtgttccatc ttccttaaaa atgcctttta ttaaactggc gactttatca
     3001 ttattaatga ctctctttaa ttctctttca atagcatcag gagtgtccat atcgactgtt
     3061 gattgagctt cagaccttac ataatttgga gaagcaagaa ctgatacttt cctgcccttg
     3121 taattcccaa cggtattttg gataactgaa ataatccagc ctttatgatt tccttcacta
     3181 tcaccatttg gagcggcaac cagggaaaat cctgagggtt gtccagcaga atttttataa
     3241 gaaaaacaaa ttctcgttgt atttcttgtt acttccatga atgctttagg gtcttcttct
     3301 gacaaaggta acgaaaaatg tttggtttga agaatttctc ctattaccgc accggtattg
     3361 tctccctgag aaaaggatgg ataaacacct tctgatagcg ccaaatcacc ttgtggattg
     3421 acagttaacc aatcagattt taattgcgca acagtaagtc catcaaaatc cacgtaacag
     3481 gttctttcaa gatctttatc gatttgctca atagttttag caacaatatt tttttgtgtt
     3541 ttggtttgtt tcaatacttc ttcaggaatt aactcaacat ctttggataa aaatacactt
     3601 tcaggcaatg aaaatatttt aggtacttgc tgataagtta aattgaattt tttaataccg
     3661 gacataatga ctggctccaa tattttctca atgcacatat tgtaacattt tattcatcaa
     3721 gaaaacatta gaatcaatgt gattatatta acaccatcat tgtctctttc gaatcaaatc
     3781 aaaaacggta tgattaatca gataacagcg aggagcaaaa taaaaaatat tcccgtgcca
     3841 tacttctttt tacacgggat atcatttaat ttagaaatca gtactgtact ttttatatac
     3901 ttcatccgct gctttcaatg attccaaaag ttgtttcatt ttatctagag gccaactatt
     3961 gggaccatca cttagtgcct tatccggatc agggtgagtt tccataaata accctgatat
     4021 tccaacagct acagctgctc ttgcaagagc cggaataaat tctctctgcc ccccggatac
     4081 accattattg cccccgggca attgaaccga atgtgttgca tcataaacca ctggacagcc
     4141 ggtttcacgc atgataacga gggaacgcat atcagaaacc aaattattgt agccgaagct
     4201 cactcctcgc tcacaagcca taatttgctc attaccttgc gcctttgcct tcgcaataac
     4261 atgtttcatt tcccagggag caagaaactg tcctttttta atattaaccg gcttattcat
     4321 ggcagcaact ttttggataa aattggtttg tctgcatagg aaggctggcg tttgtaatac
     4381 atctaccaca ctggagactt caaataaggg cgtgtcttca tgaacatcag tcaaaacagg
     4441 aacacctatt tgtgatttaa ccttttccag aatcgataac cctttttcaa agccaggccc
     4501 cctgtaactg gaaatagatg aacggtttgc tttatcaaaa gaagacttat agatgaaagg
     4561 gatattcaac tgactgcaca tttcctttaa atatcctgcg gtctctaaag ccaattcttc
     4621 actttcaata acacaagggc cggcaatcaa aaacaatggc ttatccaagc ctgcttcaaa
     4681 accacataat ctcatttttt gtccttctct tgatggtgaa tacgagctgc aagtacaaac
     4741 tccttaaata gaggatgact atctcttgga tttgatgtga attcaggatg aaactgacaa
     4801 gctaaaaacc aggggtggtc cgctaattct atcatctcaa ccagtgaatt atctgctgat
     4861 cgccctgaaa taattaagcc atgttttacc aaactatcca catacttgtt attgacttca
     4921 tatcgatggc gatggcgctc tataatttgt ggtttgccat aaacttttct tgccagagtg
     4981 ccttcagcaa gatgacagta ttgtccgccc aaacgcatgg ttccacccaa gtcagtattt
     5041 tcgtccctga tctgcttgct gccatcggca tccatccatt cactaattaa accaagcact
     5101 ggatacaatg tttctttatt gaactcggtc gaattagctc ctgtcaaacc gactacattt
     5161 ctcgcaaatt caataacagc tgtttgcata cccaaacata tccctaaaaa aggaacttta
     5221 tgctcccgag catattggat agccttaatt ttgccttcga ctcctctctc accaaaacca
     5281 ccaggtacca aaatagcatc aatggattca agcaataaag cgccatgctt ttctatcatc
     5341 tcagcatcaa aatagataat ttctactttt gtttcagtat gaattccagc atgcaataaa
     5401 gcttcattaa tcgacttgta ggcatcattc agctccgtat atttacctac catagcgatt
     5461 ttcactgtca tcgtttgaac tgcttgcata tcaactactc gttgccactc gctcaaatca
     5521 gcctgcttag cttccaggct tagtttctta acaacaattt cgtctaaatg ctgcgcatgt
     5581 agaatcatag gtatttgata aatactattc gcatcctcaa gagaaatcac agcttctttt
     5641 tcaacattag tgaatagtgc gattttagcc ctgtctgcca tggataatgg tttttctgaa
     5701 cggcaaatca atacatctgg ctggatacca atagaacgca attcctttac cgagtgttga
     5761 gttggtttgg ttttggtttc cccagaagta gcaatataag gaaccaaagt aagatgaata
     5821 aatatcgctc gttgtgaacc taactcaata cgcatctgcc taattgcttc aagaaagggt
     5881 aatgattcaa tatcaccaac agttcctcct atttcaacca tagccacatc gaatgcatcc
     5941 gcaccgagtt tgatacagcg tttgatttca ttagtaatat gaggaataac ctgaactgtc
     6001 cctcctaaat aatcaccacg tctttctttc ttaattacat tttcgtaaat ttttcccgat
     6061 gtgaaattat tacgcttggt cattgttgtt ttaacaaagc gctcataatg tcccaaatcc
     6121 aaatcggtct ccgccccgtc attcgttacg aaaacctcgc catgctgaaa gggactcata
     6181 gtaccaggat caacgttaat gtagggatca agctttatca acgtaacgcg aagaccacga
     6241 gcttcaagaa tagctgcaag agatgcggct gctatgcctt ttcctaaaga agacacaacc
     6301 ccgccagtaa taaaaatata ttttgtcatt ttttgacccc gctcaaaatg ccgaatccac
     6361 caatttggtg ttcaatttaa tttgcatgta aacgggattc aattctatca aaatgaagat
     6421 ataaataaca gaaatttgag caaaattaat agaactattg caattgttgt ttaatagaac
     6481 gcatggaaac cgcacaatct atcgcatctc tccctttatg gccgtgagtt cctcccactc
     6541 ttgccaaagc ctgctcatca ttttcagtag ttaatacacc aaagataacc ggaatattat
     6601 aatctaacat aaccctttgg catccctggc tgacctgatc acaaacgtaa tcataatgag
     6661 aggtttcacc acggataact gctccaagtg ccactatgac ttctactttt tgttttttcg
     6721 ctagcaactg tgcaacaaac ggaatctcta ctgcgccagg aacctcaact aaggtaatgt
     6781 cctccgattt aaaacccagt tcagtcaatc tatctaacgc cccttttttt aatgcagagg
     6841 ttatcagttc attaaaagta cttacaacta gcgctattgg aaacgtgaca ccatcactaa
     6901 caattgtttt tatctctctc atgtttatcc taatcaatgg ctaataaatg ccccagtttt
     6961 atttttttgg tttttaaata actgtgattt tctctggagg gctcaatttc caaaggaatg
     7021 cgttgagtaa tcttcattcc ataacgctca agagaagcga tttttaaagg attatttgtc
     7081 agcaacctta atgcttcaac ccctaaatat ttcagtattt ggtatgctac ggcataatct
     7141 ctatcgtcag cgggcaatcc taattccaga ttagcatcga ctgtatcaaa cccctgctct
     7201 tgcaaagcat acgcctttaa tttgtttgcc aaacctattc cgcggccttc ctgacgcaaa
     7261 taaattaata taccgccttc cgctccgatc tgggataaag acaactccag ttgctttcca
     7321 caatcacatt ttctggagcc aaaaacatca cctgttatgc actcagaatg aattcgaacc
     7381 aaaggcactt gattaccata caaagggggt ttaaccaaga caaaatgttc acaggaatct
     7441 atctgattct caaaaacagt catagtaaaa acaccgttct cctccagagg aatttgtgtg
     7501 gttgcaacgg gatgaatcag attttcatgc cgtactctgt actgaattaa atctttaatg
     7561 gtgactaaag ggatttgatg tttttttgaa aaaagagcta attcatcacg tctgctcatt
     7621 gttccatctt cattaatgat ttcacaaatc actgcggccg gggtcaatcc ggcaagtttt
     7681 accaagtcaa cactgccttc tgtttgacct ggtctttcta aaactcctct ctttctagcc
     7741 cgtaaaggaa aaacatgtcc aggtgaaatg atatcagatg gcccgctttt tggatcaata
     7801 gccacttgaa ttgttcgagc tctgtctctt gctgagatgc ctgtggagac accgttagct
     7861 gcttcaatcg aaaccgtaaa cgccgttcca tacggcgatt tgttatgccg agccatcatg
     7921 ggcagttgga gtttgtctat caattcttca gccataggca aacaaattaa accgcaacca
     7981 agacgagaca taaaattaat ggcttcagga gtagcgtgct cagcagcaat caccaaatca
     8041 ccttcattct ctctatcttc atcatccatt agaataatca ttttaccggc tttaagtgtc
     8101 gccacagcct cttctattgt tgctaatgaa tgtttcatga cacttcctct aggttcaatt
     8161 tcccgataat ttgaagctga tgagccacaa tacgggtaag ataatcaaat tcaatattga
     8221 cttgctggcc cactctcaat gaaccgagat tggtcattga taatgtatgt ggcaccagca
     8281 tgagtccgac ggtttgttct gatacagtat taatagtcaa gctaactcca tctactgtaa
     8341 tgctaccttt gggtattaaa taatgattat gttctgggat aaaaccatcc aaatccatcg
     8401 cgataaattc attaacagca cgaatggatt tcactttggc cctggtgtct acatgacccg
     8461 atacataatg accaccaaag cgggttgatg ccagcatagc tctttcaaga ttaaccatgt
     8521 cgccagatga taattttgac aatgtggtaa catgcagagt ttcaggtgaa atatcaaaac
     8581 acagttcagg aaaatctgct ggcaaaaggg ttaaacaaac cccatttaca gcgatactct
     8641 caccaggttt caaatcctga tagcttgaag atacgactaa acgactagaa ttttctcctt
     8701 gcctattcat taatactttg cctgtatttt ctatcaatcc ggtaaacact catcctcctg
     8761 ccaatccaaa catgctatca tattgtcacc catagcttgc caactcaccg tatgagcttt
     8821 ttcaaaaatc ccctgttgtt gataagctga aatcgcttga tgccctaaac tgccgggaac
     8881 taaatacaaa taggttctat gcaccaatcc cttactatgt aacgcagaaa aaatagctcc
     8941 gcccgcctca acccatacgt catgatatcc cagttgacca agatgagtta ttattgcctc
     9001 caggtccatc aacccatttt gcaaaggcat cgcataatga gtgctattag gataagtctt
     9061 tgcctgattt gcttgtggtt gatgataaat gtggcaatgt tttgctgtcg aaaaaatgat
     9121 tgtgtcatca gacaaactta aattgcggtc aacaatggca actggctttg ctaccaatcc
     9181 actatctaat ctggcgttca ttcttggatt atccatttga actgtcttgg cactggttaa
     9241 tatgacatca ctggcagcac gcatttgatg agtgaactgt tcgcataaag cattggacaa
     9301 aaccactcga gctccctcag actttccaat tttaccatca aaagtttgtg ctattttaac
     9361 agtgactctt ggtttatggg tcatagtcca ataagcatag cttttataga acacatcaat
     9421 tgcctttaca ggaatatgcc tgacttgtat tccatgttca cgtaattttt ctgaggagtt
     9481 acttttggca acaatggggt ttggatcaaa ataggaaaaa acaacctcct ctataccatg
     9541 attgataata gcatcaacac aagggggagt tttgccccaa tgattacaag gctccagcgt
     9601 gacataaagc gtaacccctg gcgtttgggg tggaatttgt gataacaata attgttctgc
     9661 atgcggtgta cctgcacctc tatgccatgc ctgtgctata atattaccat tttgcacagc
     9721 aaccgcccca acgctgggat tgggggcaca aaagcctctc cccagtttgg cttgttcaag
     9781 tgctgcaagc agaaactgtt cgtgcatata ttaaccacct tcttaatgtg gtcgaatgat
     9841 aacaaattat tcaattattg agtagcctaa tgattaaatt gaacttaaat tataagttaa
     9901 tcacgaaaaa gacattacct aaactatttt actggggttt ggggatcgtc ttcttttttt
     9961 tagcacctac aactaatgcc gggagtcttt caccctatac aaccaaagaa ctggaacaat
    10021 tagaaagaga atttattcaa cttattaatc agtcggacag cgtagagcgc aatcctttgg
    10081 ccagtcaata catcaactcc ataggaaaaa aactggcaca ttatggccgt atcaaaacac
    10141 cttatttttt tattgttaaa tccaaagaaa tcaatgcatt tgcaggtcca ggagggtaca
    10201 taggcattaa tacccaactc attttaacga gcagcaatga aagtgaactg gcagctgtaa
    10261 tggctcatga aattgcgcat gttcgcttac atcatcttta cagcattata gaacaccaaa
    10321 aacagatgcg tgttccatta ttagcctcta ttctggcgtc tgcagccttg ggcgtcttga
    10381 atccaactgt cgggatgggc gctatgatgg catccgtttc cggattttcc caggacaaca
    10441 tcaattttac acgctcaaaa gaaaaagaag ccgacagaat tggtatagac atgctgatta
    10501 aggcaggctt caatcctcaa ggtatggccg cattttttaa taaaatgcaa gaaaattccc
    10561 gttattatta cactgccaat attccagcaa tactgcggac tcaccccctg gatgctgaca
    10621 ggatagctga ggctgaaaac agaaccagcc gtttgcctaa aaaacattat actgattctt
    10681 tggactatgc cttatttaaa gagttaatca gagtttctgt cgcctctaat aataaacaaa
    10741 ttcttgatta ttatgagcat cattgccata aaaaaaatcc tgccactgct tgccagtatg
    10801 gatacgtact tgccttgtta aataataaca attttcaaaa agcagctgaa aaattaaacc
    10861 ctttactgtt acaggataat agtaatctca attttcaaat tgctatggct caagctgaat
    10921 taggtttatc acaatatcaa tcagcactct cccgtcttgc tgaattacaa aataattttc
    10981 ctgacaatta tgctgctcta atggcatacg cccaaggatt gttaactgca aaccaacctc
    11041 aaaaagctac agccgtactt cttaagggta gtaggcaata caaagaggac ttaccgcttt
    11101 gccgagaatt ggcaagggct caagctgaat cccatcagaa aagttatgcc tattttactc
    11161 aagcacaatg cttgctactt gaggggcaaa aaagaggtgc tattgctcaa ttaaaagtgg
    11221 cgcagaaact ggccaaaagg gatgcttatt tatccgcaag aatttcagca aa
//
LOCUS       DACTJE010000102        11255 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.102, whole genome shotgun sequence.
ACCESSION   DACTJE010000102 DACTJE010000000
VERSION     DACTJE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11255
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.102"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(152..2005)
                     /locus_tag="I9760_002023"
     CDS             complement(152..2005)
                     /locus_tag="I9760_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT5043786.1"
                     /translation="MSIVSLNGASLILAGNCILDRADLQIQPQDRIALVGRNGAGKST
                     LLKILQGELVLDGGQIQRSSGLRIAGLVQEVPSAEGESVYHFLVKSLGETGEVLSQFH
                     EFTKQGDMDKLAKCQQRMDNLNAWHLLPEIETMASRLGIDIHEQMSNLSGGMKRRVLL
                     GAALLAKPDLLLLDEPTNHLDVEAIEWLENYLKSFSGAVIVVTHDREFLSQIATSILE
                     IDRGKLYLHHCNYEAYLDRRESIRLSEQKQNDLFDKRLAEEEAWIRTGIKARRTRNEG
                     RVRALKAMREQYKARRNQLGTVKSLNLDVSRSGALVVEANKVSYTINDKPIIKDFSLL
                     MTRGDKLGIIGPNGCGKTTLVRLLLGEIKPDAGTIKLGTGLQVAYFDQLRRHLLEDQT
                     VMFNVGEGADYVTINGKQKHVASYLKEFLFSSDRFNQPVSSLSGGERNRLLLAKLFAK
                     PVNLLVMDEPTNDLDIETLELLEAVLADYPGTLILISHDRAFINQVVTSVLVYEGNGK
                     FNEFVGGYDDYKKHQLTRNEQQTKSPVSKRSSPEKAKNLSFNEQRELSKLPQQIEAVE
                     KKIEALHSQMASPEFYQQDAKLISEVTQLLAKEEALLNQYFARWEELEEKK"
     gene            complement(2066..2557)
                     /locus_tag="I9760_002024"
     CDS             complement(2066..2557)
                     /locus_tag="I9760_002024"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HAT5043787.1"
                     /translation="MSDDQYARTGRLMFLFVWIIFFIGLFLFFYYYDKPESTIFVASR
                     TEYVLSADNEGHYWIKGKINEYPVEFLVDTGATLVAIPQGLAKDLKITGRYPVTIETA
                     NGKVTGFLTRLQHLSFGEFHLQNVKAVIIPQNDDNTVLLGMNVLSQFHMIQQGKQLIL
                     KRQ"
     gene            complement(2560..2946)
                     /locus_tag="I9760_002025"
     CDS             complement(2560..2946)
                     /locus_tag="I9760_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAT5043788.1"
                     /translation="MQPINTKLAPKAIGTYSQAIKSGEIVFLSGQIPLDPETMQICSD
                     DIKLQITQVLENLSAVCEEAGGSLANIVKLNVYLTDLSHFPLINEAMSRYFSEPYPAR
                     AAIEVSALPKGAKVEMDGILVLSESA"
     gene            complement(2946..5069)
                     /gene="spoT"
                     /locus_tag="I9760_002026"
     CDS             complement(2946..5069)
                     /gene="spoT"
                     /locus_tag="I9760_002026"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAT5043789.1"
                     /translation="MSYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEP
                     YITHPVAAALILARMRLDYQTIMATLLHDVVEDTSISKEELVNQFGEEVTALVDGVTK
                     LTKIKFESKAEAQAENFRKMVLAMVKDIRVIIVKLADRLHNMRTLGSMPAAKRRRIAI
                     ETLEIYAPIANRLGMHSIYIGLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEK
                     DLQHALSQLNIPYEQVFGRQKHLYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYR
                     ILGALHCTYKPVPQRFKDYIGIPKANGYQSLHTTLFGPFGVPLEVQIRTREMDKVADN
                     GVAAHWIYKSSGLEVNEAQLRAREWVQSLLEMQRSTGSSLEFIENVKIDLFPDEVYVF
                     TPKGHIMELPKGATPVDFAYTVHSGVGNSCVAAKVNRRLVPLSIPLSNGQTVEIITAP
                     GAHPNPAWLNFVVTGKARSNIRHFLKSQQHAESIVLGKRLLEQSLAELSSDYAKVPPE
                     TLQALLSDLHYKTVDDLLYAIGIGNQMAMVIAKRLVVSQDANESDKGNKKPLAIKGTE
                     GMVVHFGECCQPIPGDNIVGKFQQGRGIIVHASDCTTLKTTRSNPEQFIALRWDEQVQ
                     GEYWVDITVDVANERGVLAALATAISEADSNIGNINVDPRDGRHNAVTFSISVRDRTH
                     LARVMRRLRANKVVMRLYRKKQGEI"
     gene            complement(5237..5440)
                     /gene="rpoZ"
                     /locus_tag="I9760_002027"
     CDS             complement(5237..5440)
                     /gene="rpoZ"
                     /locus_tag="I9760_002027"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAT5043790.1"
                     /translation="MARVTVEDCLEHVKNRFELVMVATKRARQIAVRGDQPMVEWEND
                     KPTVVALREIAEGYVTADILDED"
     gene            complement(5491..6120)
                     /gene="gmk"
                     /locus_tag="I9760_002028"
     CDS             complement(5491..6120)
                     /gene="gmk"
                     /locus_tag="I9760_002028"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAT5043791.1"
                     /translation="MVSDNSGNLYIVAAPSGGGKTSLVKKLIEMVGEIEVSVSHTTRP
                     MRPGEKEGVDYFFIDEKQFISMVNEGAFIEHAKVFNHWYGTSVAQINKRLQFGIDVVL
                     DIDWQGAEQIRHAYPDAVSVFIIPPSLDALKERLMNRRQDKDHVISERMTKAQDELGH
                     YPEFDYLIVNDDFEKAAMELQSIVIANRLRIEKQVNKQAKLLSFLLSSQ"
     gene            complement(6126..6992)
                     /locus_tag="I9760_002029"
     CDS             complement(6126..6992)
                     /locus_tag="I9760_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAT5043792.1"
                     /translation="MIHSMTAFSRIQKQFDVGDFCWEIRSVNHRYLDVSFRLPESFRF
                     LEAELRTLLRDKIHRGKLECHLKYKDTSCDNRSMLINIGTINALLDLSSKLSASHHLA
                     NDMTVSQVLSWPGVVEANLLDMEELSQIALELFQKSIDKLCEVRAGEGRSLKAYVEDR
                     LQALRQEVMKSKSIVVSMSAKTKDKLLTRLHELQMDVPEARVEQEIALLLAKLDVSEE
                     LDRLETHIAEVSRTLNAEKVTGRRLDFLMQELNREANTLSSKSDSAELTLCAVEMKVL
                     IEQMREQIQNFE"
     gene            7293..8000
                     /gene="rph"
                     /locus_tag="I9760_002030"
     CDS             7293..8000
                     /gene="rph"
                     /locus_tag="I9760_002030"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019216256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAT5043793.1"
                     /translation="MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIV
                     EGVPRFLKGKNQGWITAEYGMLPRATHSRTEREASKGKQGGRTLEIQRLIGRSLRACI
                     DLKVLGENTITLDCDVIQADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPAFNYVA
                     AVSVGIYRGQPVLDLDYAEDVLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSML
                     SLAEIGIPQLIEIQKNA"
     gene            complement(8330..9364)
                     /locus_tag="I9760_002031"
     CDS             complement(8330..9364)
                     /locus_tag="I9760_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAT5043794.1"
                     /translation="MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEH
                     KDVIKIIYDIMNDRQRKEYEEFFETDFSFEIANLARFRVNAFNQSRGAAAVFRTIPSE
                     ILSMEDLGLPPIFKEMASFSRGLVLVTGPTGSGKSTTLAAVIDYINSSRYEHILTVED
                     PIEFVHQSKKCLVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAA
                     ETGHLVFGTLHTNSATKTINRIIDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGR
                     VAALEIMMCTGAIRNLIREDKIAQMYSSIQTGQAKGMQTLDQHLTELVNKNIISKHTA
                     HEAALNKSLF"
     gene            9528..10223
                     /locus_tag="I9760_002032"
     CDS             9528..10223
                     /locus_tag="I9760_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAT5043795.1"
                     /translation="MSISQNINKVRELITQTESSSNRQPNSVLLLAVSKEQSPELIRE
                     AFQQGIRHFGENYFQEAQEKILALRDLPICWHFIGPIQSNKTKGIAKYFNWVHSIDRN
                     KTAKLLNQFRSEQLPPLNVCLQINLTGETTKSGIPPEHAIDLALEVKQLPNLQLRGLM
                     TIPPQQNNMQTQYNLFLKLNQLKESINKALHLKMDTLSMGMSDDLVPAIKAGSTIVRI
                     GRAIFGERKGKLL"
     gene            10220..11008
                     /locus_tag="I9760_002033"
     CDS             10220..11008
                     /locus_tag="I9760_002033"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAT5043796.1"
                     /translation="MNISFIGYGNMAKAIACHLQKQNKYSLSAASPSLSAGISKENIR
                     THYDNKEVAKDADVIILAVKPAQMREVVNEINPFISSGCLLISVAAGLSLTWFAKYCK
                     KGQAVVRTMPNTPAIVGFAATSMIANEFVNEKQKKWAEQLFSSIGITDWVKDEEDMDT
                     LTALSGSGPAYVFLFLEAMINAGVQLGLEETVAKHFAIQTFSGSLQLAQHSQLSLTEL
                     RKKVTSPGGTTAAALEILHGDLDELIFSAMKAAKQKAHELGKVE"
BASE COUNT         3432 a         2410 c         1959 g         3454 t
ORIGIN      
        1 taatgaattc ttgagagaag aaaaccataa ttgttgcaaa taaaataacc aaagccaagg
       61 ttaaaagcat tttaaatcct atttttaatt ttatttaaag atatcttttt attgtttttc
      121 actttaactt atcaattaac caaatacttt tttatttttt ctcttccagc tcttcccatc
      181 gagcaaaata ttgatttaac aatgcctctt ccttggctaa cagctgagtt acctctgaaa
      241 taagcttggc atcttgctga taaaattcag gtgaggccat ttgcgaatgc agtgcttcaa
      301 tttttttctc tacggcttca atctgttgcg gcaacttcga taactcacgt tgttcattaa
      361 aagataaatt tttggctttc tctgggctgc tccgttttga aactggagat ttcgtttgct
      421 gctcatttcg tgttaattga tgttttttat aatcgtcata accaccaaca aattcattaa
      481 acttaccatt tccttcataa accaacacac tggttactac ttgattaata aacgctctat
      541 catgactgat tagtattaaa gtaccaggat aatcagctaa tacagcttca agaagttcca
      601 gagtttctat atccaaatca ttagttggtt catccattac gagtaaatta accggtttgg
      661 caaaaagttt tgccagtaat aatctgtttc tttcccctcc agacaaggaa gatactggct
      721 gattaaatcg atcagaggaa aataaaaact ctttcaaata actggcaaca tgtttttgct
      781 taccattaat tgttacataa tcagcccctt cccccacatt aaacatgact gtttgatctt
      841 caagcaaatg tcggcgtaac tgatcaaaat aggctacctg aagccctgtc cctaatttta
      901 tagttccagc atcaggctta atttctccca gaagtaatcg aactaaagtg gttttgccac
      961 aaccattggg gccgataatc ccaagcttgt ctcctcttgt cattaataaa gaaaaatctt
     1021 ttataatggg cttgtcatta attgtataac tcactttgtt tgcttctact acaagagcac
     1081 cagaacggct tacatccaaa tttaaggatt tcactgttcc taattgatta cgacgtgctt
     1141 tgtactgctc tctcattgcc tttaaggccc tgactctgcc ctcattacgt gtgcgtcttg
     1201 ccttgatacc tgtacgtatc caggcttctt cttctgcaag ccgtttgtca aacaaatcat
     1261 tttgtttttg ttcgctaagc cttatcgatt cacgcctgtc aaggtaagct tcataattac
     1321 aatggtgtag atacaatttt ccacgatcga tttcaaggat gcttgttgct atctggctta
     1381 agaattccct gtcgtgagtt actactatta cagcaccaga aaaggatttt aaataattct
     1441 ctagccattc aattgcttcg acatccaagt ggttggtagg ttcatccagt aacaataaat
     1501 caggcttggc aagcagtgcc gcacctaata atacgcgtcg tttcataccg cctgagagat
     1561 tagacatctg ctcatgaata tctattccca atcggcttgc catcgtttca atttcaggta
     1621 ataaatgcca tgcattcaag ttgtccattc gctgctgaca tttggccaat ttgtccatat
     1681 ctccttgttt cgtaaactca tgaaactggg acaatacttc ccctgtttca cctaagcttt
     1741 taacaagaaa atggtacact gactctccct cagcacttgg gacttcctga actaaaccag
     1801 caatacgcaa gccggatgag cgttgaattt gtccaccatc aagcaccaac tcgccttgta
     1861 gtattttcag caatgttgat tttccagcgc cattgcgtcc aacaagagca attctatcct
     1921 gtggctgaat ctgtaaatct gcacggtcca gaatacaatt gccagccaag attaaagagg
     1981 caccgttaag ggaaactata ctcatcaaaa ctctctattt aaataaatct gtttgatctc
     2041 attttattga aaacatacag tcagttcact gtctttttaa tatcaattgt ttgccctgct
     2101 gaatcatatg aaattgtgat aggacattca ttcctaataa caccgtatta tcatcatttt
     2161 gcgggattat gaccgctttg acattctgta agtgaaactc tccaaatgac aaatgttgca
     2221 aacgagttaa aaatccagtt accttgccat tggcggtttc tatggtaaca ggatatcgcc
     2281 cggtaatttt taaatcctta gccaggcctt gcggtattgc taccaaagta gcgccagtat
     2341 ccactaaaaa ctctacagga tattcattaa tcttaccttt tatccaataa tgtccttcat
     2401 tatcagcgct taatacatac tcggtacgac tagctacaaa aatggtactt tctggcttat
     2461 cgtaatagta aaagaataaa aacaacccta taaaaaatat aatccaaaca aataagaaca
     2521 tcagacgccc tgtgcgagcg tattgatcat cactcatgat tatgcggatt cagacaaaac
     2581 cagaatccca tccatttcaa ccttggcacc ctttggtaat gcggagactt cgattgccgc
     2641 tcttgcagga taaggctctg aaaaatatcg actcatcgct tcgttaatta agggaaaatg
     2701 acttaaatca gtgagataga cgtttaattt gacaatattg gccagactac caccagcctc
     2761 ttcacataca gctgataaat tttccaggac ttgagtaatt tgtaatttaa tatcatcgct
     2821 gcagatttgc attgtttcag gatctaatgg gatctgccct gaaagaaaaa cgatctcacc
     2881 actttttatc gcttgactgt acgtgcctat cgcttttggc gccaattttg tattgattgg
     2941 ttgcattaaa tctccccttg tttttttcga tataaccgca taactacttt atttgccctt
     3001 aatcgacgca tgactcgagc caaatgggtt ctgtcgcgaa cactgatgga aaaagtgacc
     3061 gcattatgac gaccatctct gggatccaca ttaatattcc ctatattaga gtctgcttct
     3121 gaaatagcag ttgctaaagc agccaataca cctctttcgt tggctacatc tacagtaatg
     3181 tctacccaat attcaccctg aacctgctca tcccaacgca aagcaataaa ctgttccgga
     3241 ttactacgag ttgtttttag tgtagtgcag tcactggcat gaacaataat gcctctgcct
     3301 tgttgaaatt taccaactat attatctcca ggtattggtt ggcagcattc accaaaatgc
     3361 acaaccatcc cttcagtgcc tttaatagcc aaaggttttt tattgccctt atccgattca
     3421 ttggcgtctt gcgaaaccac caaccgcttg gcaataacca ttgccatctg attacctatt
     3481 cctatggcat acaataagtc atcaaccgtt ttatagtgta aatcgctgag caatgcctgt
     3541 aatgtttccg gaggcacttt ggcgtaatca ctggataatt cagccaaaga ttgctccaat
     3601 aatcgtttgc ctaaaacaat agactcagcg tgttgttggc ttttcagaaa atgacgaata
     3661 tttgatcttg cctttccagt cacaacaaaa ttcagccaag ccgggttggg gtgtgctccc
     3721 ggggctgtaa taatctctac ggtttgacca ttggataaag gaatgcttaa aggaaccaaa
     3781 cgcctgttca ctttcgctgc aacacaacta tttcccactc cagagtgtac cgtgtaagca
     3841 aaatccacag gagtggctcc tttcggcaac tccataatgt gccctttagg agtaaaaaca
     3901 tatacttcat caggaaataa atctatttta acgttttcga taaattcaag agaactgcct
     3961 gtgctccttt gcatttcaag caaactttga acccactctc tggcacgaag ttgagcttca
     4021 ttgacttcca atccggaaga tttatagatc cagtgcgctg ctacaccatt atcagctact
     4081 ttatccatct ctcttgtgcg tatttgaacc tcaagaggaa caccaaaagg cccaaacaaa
     4141 gtggtatgca atgactgata gccattggct ttgggaattc cgatatagtc tttaaagcgt
     4201 tgtggtacag gcttataagt acagtgcaaa gctcccaaaa ttctataaca ggagtcaatg
     4261 tcttctgtaa tgatgcgaaa tgcaaacaca tccgtaattt ctgtaaagga tgctttcttt
     4321 tgcttcattt ttctataaat actatataaa tgcttttgcc tgccaaatac ttgctcatag
     4381 ggaatgttca actgcgataa ggcgtgttgc aaatctttct ctatggtctg tgtcaactct
     4441 cttcgattac cccgagattt ttcaacagca gatttaattg ccctgtaacg cataggatat
     4501 aaagcttgaa accctaaatc ctcaaggccg atataaatag aatgcatacc caaacgattg
     4561 gcaatagggg cataaatctc gagcgtttca attgcaattc gtctgcgctt ggctgcgggc
     4621 attgatccta aagtgcgcat attatgtaag cgatcagcca atttaacgat aataacgcga
     4681 atgtccttaa ccatagccaa aaccatcttg cgaaaatttt ccgcttgagc ttcggccttt
     4741 gattcaaatt taattttggt gagtttggtg acgccatcaa caagggcagt gacttcttca
     4801 ccaaattgat taaccagttc ttctttactt atggaggtat cttcaacgac gtcatgaagc
     4861 agagtagcca tgatagtttg atagtccagt ctcattcttg ccagaatgag ggctgctgct
     4921 acagggtgtg taatataagg ctcacctgaa cgacgcattt ggccatggtg tgctttttca
     4981 gccaccaaat aagcttgata acatttctct atttgcgcct gctcaaggta gcatttaagc
     5041 tcttcatcga gctctttaaa gtagctcacg cagtactccc tagtatccaa catggctatt
     5101 tatacctcac attactcaaa atacggtttt atgcctcgcc cttgttccct ttgctttgct
     5161 taaataccat aacttgatta ccaagaggta tattttagaa tggctaatat tcccaaggca
     5221 aagtcaataa ctcattttaa tcttcatcca aaatgtccgc tgtaacataa ccttcggcaa
     5281 tttccctaag tgctactaca gtaggcttat cattttccca ttcaaccatg ggttgatcgc
     5341 cacgaactgc aatttgcctt gctcgtttgg tagcaaccat aaccagctca aaccggtttt
     5401 taacatgttc taaacaatct tcaactgtta ctcgtgccat aaaatccacc tctaggttat
     5461 tagaacaaat actaactcaa atcttttatt ttactgcgat gacaagagga aagaaagcaa
     5521 tttggcttgt ttatttacct gtttttcaat tcgaagccta ttggcaatta ctatcgactg
     5581 taattccatt gccgcctttt caaaatcatc attaacaatc agataatcaa attcaggata
     5641 atggccaagt tcatcctgtg cttttgtcat tctctcacta atcacatgat ctttatcttg
     5701 tcgcctgttc atcaagcgtt cttttaatgc gtctaaagaa ggaggaatga taaatacact
     5761 cactgcatcc ggataggcat gtctaatttg ctccgctcct tgccaatcaa tatcaagaac
     5821 gacgtcaatt ccaaattgta accgtttgtt aatttgagca actgaggtac cataccaatg
     5881 attaaatact ttggcatgct caataaacgc cccctcattc accatagaaa taaattgttt
     5941 ctcatcaatg aaaaaataat ccaccccttc tttttctcca ggtctcatag gtctggtcgt
     6001 atgagaaact gacacttcaa tctcacccac catttcaatg agttttttca ccaaacttgt
     6061 tttccctcct ccggagggtg ctgcaacaat gtataaattt cctgaattat cactaaccat
     6121 atcatttact caaaattctg aatttgttca cgcatctgct caatcaatac tttcatttca
     6181 acagcacata aagttaactc tgctgaatcc gattttgagc ttagggtatt cgcctctcta
     6241 tttaattctt gcattaaaaa atccagtctt ctacccgtca ccttttctgc attcaaagtg
     6301 cggcttactt ccgctatatg agtctctaac ctgtccaact cttcactcac atcaagtttg
     6361 gctagtagca aggcaatttc ttgctctaca cgcgcctctg gaacgtccat ttgcaattca
     6421 tgcaaacgcg taagtaattt atctttagtc ttggcactca ttgaaaccac aatgcttttg
     6481 gatttcatta cttcttggcg caaagcctgc aatcggtctt caacatatgc ctttaatgac
     6541 ctgccttccc cagctctgac ttcacacaat ttatcgatac ttttctgaaa taactccaaa
     6601 gcaatttgag acaattcctc catatccagc agattagcct ccacaacgcc tggccatgaa
     6661 agcacttgac tgacagtcat atcattagcc agatgatgag aagcagataa tttactgctt
     6721 aaatcaagta atgcattaat cgtacctata tttataagca tagaccgatt atcacaactt
     6781 gtatccttgt actttagatg gcactccagt ttaccccgat gtatcttatc ccgaagcaaa
     6841 gttcttaatt ctgcttcaag aaatcgaaag gactccggca aacgaaaaga gacatcgaga
     6901 tagcgatggt taacagacct gatttcccaa caaaaatcac ctacatcaaa ctgcttttgt
     6961 attcgagaaa aagccgtcat actatgaatc ataaagaata cctacttttt aaccttgcgc
     7021 gaatatacct gaaattaaaa gaattgcaca gggattgccc atcaattaat tgcaacaaac
     7081 ttctgtatca ggtcctgaaa gtcaggcctt aaatcaaact tgaaacttaa aacaaactgt
     7141 ttctaacgta aagaatggaa acaaatcgaa tggtttgatt aaaaatattt ttcagcaaaa
     7201 atagagagga acgtacaaat agcgtatgta tttagggtgc aactcaagta ttaaagagtt
     7261 atacttgggc attttacaga ggagtatctc taatgcgtcc cagtaatcgt gaacatgatc
     7321 aacttcgtcc cgttacaata acacgtaatt ttaccaatta tgccgaaggc tctgtattag
     7381 tcgaatttgg tcagacaaaa gtgatttgta acgcatcaat cgtcgaagga gttccccggt
     7441 ttttaaaagg taaaaaccaa gggtggataa cagctgaata tggcatgctc ccacgtgcaa
     7501 ctcatagccg taccgaaagg gaagccagta aaggtaagca aggtggcaga acattggaaa
     7561 ttcaacgtct catagggcgt tcattgagag catgtatcga tttaaaggtt ttgggcgaaa
     7621 atactattac tcttgattgt gatgttattc aagcggatgg aggaaccaga acagcagcta
     7681 taactggcag ttgtgtggct atgcgagatg caattcactg gatggttcaa cgtgaaaaaa
     7741 taaagaagat gcctgctttt aattatgtag ctgccgtatc tgtaggtatt tacaggggtc
     7801 aacccgtgct ggatttggat tatgcagaag atgttcttgc tgaaacagat atgaatgtgg
     7861 ttatgaatga acaaggacat tttattgaag ttcaaggcac tgctgaagat aactccttta
     7921 accgggaaca actgaacagc atgttgtcat tggctgaaat tggtattcct caattgattg
     7981 aaatccaaaa aaatgcctga gaacaatagc ccggcataaa cagtcgcccg ctatagaaat
     8041 taatacttca aacctgagca aaagatctct gcaggaatgc tttgagctaa ctgaagagat
     8101 ctcattgcag ttattggcat tgctatctcc aaagtttttt gaggagttct ggaacttgcc
     8161 tgtactcaat tcctgtaaat accaacaccg tattgaaatc tatctggact tgcgccccta
     8221 atctctttgt taaataatgt tttaaattta gtcatttaat ttatataaac ttcctcatta
     8281 aaaacatttt ttgtctcgac cttgggtctt tttcacaagt catgtgtatt taaaataatg
     8341 acttgttcag ggcagcctca tgagcagtgt gtttcgaaat aatattttta ttaaccaatt
     8401 cagtcaaatg ttgatccaat gtttgcattc ctttggcttg accagtttga attgacgaat
     8461 acatttgcgc tatcttatcc tcacgaatta aattacgaat agcccccgta cacatcataa
     8521 tttcaagcgc cgcaacccga ccacctttat tcttttttaa taaactttga gcaacgacag
     8581 cttgcaatga ttcagaaagc atggagcgta ccatcgattt ttcttcagcg ggaaatacat
     8641 caattatcct gtttattgtt tttgttgcag aattggtatg caatgttcca aaaaccaaat
     8701 gcccggtttc tgctgcagtc atcgccaagc gtatagtttc caaatcgcgt aattcaccca
     8761 ccaatataat atccggatct tcacgtaacg ctgatctcaa agcggcatta aagctcaatg
     8821 tgtctttgtg tacttcgcgt tggttgacca gacatttttt gctttgatga acaaactcaa
     8881 tgggatcttc aactgtcaaa atatgctcat aacgacttga attaatataa tcgatgactg
     8941 ctgcaagcgt ggtacttttt cctgatccag ttgggccagt aaccagaacc agacccctgg
     9001 aaaaactagc catttcttta aaaattgggg gcaatcctaa atcttccatg ctaagaattt
     9061 cagaaggaat agttctgaaa acagcagcgg caccgcgcga ttgattgaaa gcattaaccc
     9121 tgaaccgtgc cagattggct atttcaaagg aaaaatctgt ttcaaaaaat tcttcatatt
     9181 cttttctttg cctgtcattc attatgtcat aaataatctt aatgacgtct ttatgctcta
     9241 atggaggtaa attaattttg cgtaaatcac catcaacacg aatcatagga ggcatgccag
     9301 ctgaaatatg caaatctgat gatttgtttt taactgaaaa tgccaataat tccgcgatat
     9361 ccatactcta taattcccgc caattttttc attatatatt gtagattaac tcatgaaatg
     9421 attaccagca atcatggata tctttttgta gtaccattac acttcatgca taattaatat
     9481 ttctttttat aataaagcgc ctcatctttt gattagccgg gattaatatg agcattagtc
     9541 agaacataaa caaagtaaga gaactgataa cccagacaga atcatccagt aacaggcaac
     9601 cgaacagcgt cttattattg gctgttagta aagaacaaag ccccgaatta atcagagaag
     9661 cattccaaca agggataaga cactttggtg aaaactattt tcaagaagcc caggaaaaaa
     9721 tattggcatt aagagatctg cctatctgtt ggcattttat agggcctatc caaagtaata
     9781 aaaccaaagg aatagcgaaa tattttaatt gggttcacag cattgaccga aataaaacag
     9841 ccaaacttct taaccagttc agatctgagc aactaccacc gctcaatgtc tgtttacaaa
     9901 ttaatttgac tggagaaact acaaaatcag gcattcctcc agaacatgcc atcgaccttg
     9961 ctctcgaagt aaagcaattg cctaatttgc agctaagggg actgatgaca atccctcctc
    10021 agcaaaacaa catgcaaaca cagtacaatt tattccttaa actcaatcaa ctcaaggaat
    10081 ccatcaataa ggcacttcat ttaaaaatgg atactttatc aatgggtatg agtgatgatt
    10141 tggttccggc aattaaagca ggttcgacca tagttcgaat tggacgagca atttttggcg
    10201 aacgaaaagg taaattatta tgaatatcag ttttattggt tatgggaata tggcgaaagc
    10261 aattgcctgc cacttacaaa agcaaaataa atactcattg tcagctgcct caccatcctt
    10321 atctgcgggc attagtaaag aaaatattcg cactcattac gataataaag aagtggctaa
    10381 agacgcagac gtcattatat tggcagtaaa acccgcacaa atgcgtgagg tagtcaatga
    10441 gattaaccct tttatttcat caggttgcct tctaatctca gtggcagccg gattgtccct
    10501 gacctggttt gccaaatatt gtaaaaaagg acaagcggtc gtaagaacca tgcctaatac
    10561 cccggctata gttggatttg cagcaacatc aatgattgca aatgaatttg taaacgagaa
    10621 acaaaaaaaa tgggcagaac aattattttc aagcattggg attactgatt gggttaaaga
    10681 tgaagaagac atggacaccc tgacagccct ttctggcagt ggccctgctt acgtattttt
    10741 gtttcttgaa gcgatgatta atgcaggggt ccaattgggc ctggaagaaa cggttgccaa
    10801 acactttgcc atacagacat tttcaggatc gcttcaatta gctcaacata gccagttaag
    10861 tttgactgaa ctgagaaaaa aagtaacttc tccgggaggt acaacagccg cagctttgga
    10921 aatcctgcat ggagatttgg atgagctcat tttttctgct atgaaagctg cgaaacaaaa
    10981 agctcatgaa ttaggaaaag ttgaataaca cttttgagtt aacaaaaatt ctatcgcaag
    11041 aagtcctctg ttgatttctt ttattttaat gtcagtcagg atttacaaaa accccaaggt
    11101 caaggcaaaa aatgttttta atgagggaat taagataaac taaacaactg aactaaaaat
    11161 actttttgag aaagatattg ggaacattct gttagtatta attcttgaac tgttcaagca
    11221 aacaatcatt gtggaacaag gtttgctcgc gttat
//
LOCUS       DACTJE010000103        11091 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.103, whole genome shotgun sequence.
ACCESSION   DACTJE010000103 DACTJE010000000
VERSION     DACTJE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.103"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1197)
                     /locus_tag="I9760_002034"
     CDS             complement(<1..1197)
                     /locus_tag="I9760_002034"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAT5043797.1"
                     /translation="MNNSEQFLSPRMGRVEQIHFVGIGGAGMCGIAEVLHNQGYRITG
                     SDLGESGTVQRLRSLGIQVYIGHRLENIKGADVVVRSSAVDFNNPEIVAARELMIPVI
                     PRAAMLAELMRFRHGIAIAGTHGKTTTTSLVSSLLAEGGLDPSFVIGGKLNSCGANAQ
                     LGKSAYFVVEADESDASFLFLKPMMAVVTNIDADHMDTYEGDFEKLRTTFLEFLHHLP
                     FYGLAVVCLEDEEICRILPAIQRPTLTYGFKEEAHYRAINWTQKGMLSEFVVVRPAPH
                     KQLTIQFQYPGRHNVLNALASIAIATELGVDDDSIVRGLQKFQGVGRRFQMLGEKQFE
                     KGAAIIVDDYGHHPQEILSTIDAFRRVWPERRLVHVFQPHRYTRTQSLHRQFVDVLSL
                     SDELLLM"
     gene            complement(1207..2382)
                     /gene="ftsW"
                     /locus_tag="I9760_002035"
     CDS             complement(1207..2382)
                     /gene="ftsW"
                     /locus_tag="I9760_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HAT5043798.1"
                     /translation="MRPRHLNQRGKPVSRPISLYDKWLIGAVFGLLIIGLMMVASSSV
                     MISTKYFHQPFHFLIRQACYLFVGLLLALIVVRTDSSFWEKISMPMMIGCVFLLLIVL
                     IPGIGKSVNGSRRWLALGPIGVQVSELTKLAMIFYLSGYLVRQQEAVCESIFGFIKPM
                     AILAVVSVLLLLEPDFGATVVISGTVMAMLFLAGVKLRYYFGLMLVVVTALALLAVSS
                     PYRVARLTAFLDPWADQYNSGYQLTQSLIAFGRGGWFGTGLGESIQKLLYLPEAHTDF
                     LFAVIAEELGLFGILVVITLYSILVIRGLNIGYTAYTQERHFASYTAYGLTIWLALQA
                     SINMGVNAGLLPTKGLTLPLLSYGGASMVINCIVIALLLRIDHENRWQSLGLRPLTA"
     gene            complement(2388..3731)
                     /locus_tag="I9760_002036"
     CDS             complement(2388..3731)
                     /locus_tag="I9760_002036"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAT5043799.1"
                     /translation="MNHSLYLVAGLGKTGLSIARYLKRNNKSFVVFDTRKEAPGLAEF
                     QNEFPDVPIYLQQTPDEVISQVTDVITSPGLALDTPVLERARQAGASIYGDIECLARE
                     ISAPVIAITGTNGKSTVTTLVGEMAKAAGFRVAVAGNIGTPVLDMLDDEHHYDLWVLE
                     LSSFQLDLTYSLSPVVATILNVTPDHLDRHHTMEAYTQAKQRIYRGAKAVLFNREDVY
                     TVPHQSCQADIKCISFGKDAPSMGNWGLIEQENTTYLAKGMERLLPVESILIKGVHNW
                     MNALAACALAEAAGISMQHILNVLKTFPGLPHRCQWVREVDGVGWINDSKGTNIGATI
                     SAINGIGGSMQGKIVLIAGGQGKGADFQELAQPVSEFVRSIVLIGEDADKIESALAKV
                     VPVVRASSLEGAVTIAKTCAKPGDVVLLSPACASLDMFRDFNHRGDVFTSSVRGL"
     gene            complement(3745..4830)
                     /locus_tag="I9760_002037"
     CDS             complement(3745..4830)
                     /locus_tag="I9760_002037"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAT5043800.1"
                     /translation="MLYWLTQLLQGQYHAFRVFQYLTFRSILASLTALIVGLLCGPLM
                     IRWLRGLQIGQMVRSDGPQTHLSKAGTPTMGGVLILLAITVSCLLWCDLRQTSLWLVL
                     LVTLANGLVGWVDDYRKLVLKNSKGLPGRWKYFWQSVIALVAVSYLYWNASLPVHTQL
                     TVPFFKTVTWDLGVFFPVLAYFVIVGSSNAVNLTDGLDGLAIMPIVMVAGALGVFAYA
                     SSNAVYSNYLGIPYVPNTGELTIFCSSIVGAGLGFLWYNSYPAQVFMGDVGSLALGAA
                     LGIVAVVVRQELVLLIMGGLFVIETLSVILQVGYFKYSGGKRLFRMAPLHHHFELKGW
                     SEPKVIVRFWIITVVFVLCGLATLKLR"
     gene            complement(4945..6282)
                     /locus_tag="I9760_002038"
     CDS             complement(4945..6282)
                     /locus_tag="I9760_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAT5043801.1"
                     /translation="MNLITIASLLSQSCQINADITGVSIDTRTLKPGNLFFAIVGERF
                     DGHDFIKEAEAKGASAAVVNRSIDGVHIPQFVVASPLMALAKIAAAHRQEMSCPIIAL
                     TGSNGKTTVKEMIATILPQPSHATKGNLNNHIGVPLSMLALNKQHRYAVFELGANHPG
                     EIAHTVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGEIHQGLSPTGVAIVNDDDAY
                     AHFWDDLIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLH
                     NVRNALAAAACCYAVGISGNDIQRGLAHFGGVKGRMTMLSGQNQSTVIDDTYNANLRS
                     VLTALEVLSGRPGRKIFVFGDMGELGEWTTQHHREVGVAARQLGIDKLMSCGLHSELA
                     SQAFGEGGQHFCSQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHRLVQVEG"
     gene            complement(6523..7302)
                     /locus_tag="I9760_002039"
     CDS             complement(6523..7302)
                     /locus_tag="I9760_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAT5043802.1"
                     /translation="MQANWSLILNVLLLIGVLVAIGRLMKTRRQSLNQEHYQPSLGQV
                     ENNSYGSQSYNDEIIAVRKVNREVPLDFENFDGSEKRQAIRKPSVKTEQPQLIPEEEE
                     CELNERTEPNSDCSSSTAMVFLLAKENRQFAGYELLQTVLAAGLRFGERHLFHRHQFT
                     NGQGPVLCSLAAATATGVFDLQNIGAFSVRGLCLFMQISNNPEINAERFSVMLDTAKQ
                     LSEGLDAYLLDDQRKPLTEERIARYHRYLKIEHLMDDCVTN"
     gene            complement(7307..10801)
                     /gene="smc"
                     /locus_tag="I9760_002040"
     CDS             complement(7307..10801)
                     /gene="smc"
                     /locus_tag="I9760_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAT5043803.1"
                     /translation="MHLKQLKLAGFKSFVDPTTVHFPSRLVAVVGPNGCGKSNIIDAV
                     RWVMGESSAKNLRGESMTDIIFNGSSNRKPVGQASVELVFDNSLGRLTGPFASYGEIA
                     VKRIVTRDGESFYYLNGARCRRKDITDIFLGTGAGARSYSIIGQGTVSRLIEAKPEEL
                     RVYLEEAAGVSKYKERRRETLQRIEQTRDNLTRVADIREELGKHLQRLERQAKAAERY
                     TILKEEEKICRAEIQALKWQEYSEKQTVKQRELQELAIRNEQQQSLLTSCYKERAVLN
                     EQVQDAIDQSQSIQDSLYHSGTEIARLEEAIQQQEREKKRLENDRQQMQVDSQLIEEQ
                     LKLDNEELHNCKLKAQQLEEHLHHLKMEFKEQESLWQEKQKEQSQWEKIWQEVQINSN
                     TIKNEYQLTQAKLQHLEQKRHQVLVRLEKLNLEQSAISLDDLQKNKIKLQEKHFQLKE
                     TQEFDEEQLRQANETQSDLQNQLQNTEQQLHALQDEFHRLNTELAALKAAQIAAKKEN
                     RPNKDGIAEWSDKPRLMDVLKVEPQWQSSCEIVLGDDLIAYVLDDFAELWSRWQFCED
                     HKESVVTMRALNGEKQERPRLADKIQGLVPSTVNHLEHIYAADNLEEAKAWFPDLLAH
                     ESIVTVNGIWLGKGWIKLPRQKEQDEVGVLARQQRIVELNLSVESLQQKIESIRLARD
                     SYQARLQENLNFIKLHQLNLNASNEALRNHIIAVNNQEQAIFHAEKQKDAITNEIIEL
                     ESSLEELNAEQLEMSDKIRELEGQYQASELQFKRFINEKDTWNSVLVEQNKQVESVRA
                     LLHQSELEYDREVTKIQQITDRISREKERLQRAQMRLEELTQHYLQSVAPEAEFKNQL
                     AALLIKHGEIESMLTLSREKVSQLKLQLEDQEKLILNYDQEVKRIQELMNQIQMEKQA
                     LSVRASSIQETLDEANLQAQSLLERIPSGVTQSIKEDELIAILEKIKRLGAINLAAIE
                     EFAAEQQRKVFLDEQYNDLSQALSTLETAIEKMDNETKSRLENTFDEVNTSFKALFPR
                     LFGGGRAQLELTCDNLLEAGIVVMAQPPGKRNSSIHLLSGGEKAMTAVALVFAIFQLN
                     PSPFCMLDEVDAPLDDLNVSRFCDLVKEMSQFIQFLFITHNKVTMELAEHLVGVTMRE
                     PGVSRLVAVDVKQALTME"
     gene            10976..>11091
                     /locus_tag="I9760_002041"
     CDS             10976..>11091
                     /locus_tag="I9760_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HAT5043804.1"
                     /translation="MKQQFYKLPQILISLCLLLILSTPIQAEPPNLSLIRKE"
BASE COUNT         3331 a         2633 c         1910 g         3217 t
ORIGIN      
        1 catcaataac agctcatccg ataaacttaa aacatcgaca aattgtctat gcaaggactg
       61 tgttctggta taacgatggg gttgaaagac atgaaccaaa cgtctctcgg gccaaacacg
      121 tctaaaagca tcaattgtgg ataaaatttc ctgtggatga tgtccataat catccacaat
      181 aatcgccgcg cctttttcga actgtttttc accaagcatt tgaaagcggc gtcccacccc
      241 ttggaatttt tgtaagccac gaacaatgga atcgtcatca actcccaact cagtggcaat
      301 tgctatagaa gctaatgcat ttaatacatt gtgacggcct ggatactgaa attgtatagt
      361 cagttgttta tgtggtgcag gtcgaacgac cacaaactca ctcagcatgc ccttttgagt
      421 ccaattaatt gcccgataat gagcctcttc tttaaaccca taagttaagg ttggtctttg
      481 aatagccggg aggatacggc aaatttcttc atcttccaaa caaactactg ctaatccata
      541 aaaaggcaaa tgatgcaaaa attccaaaaa tgttgttctt aatttttcaa aatcaccttc
      601 ataagtatcc atgtgatcag catcaatatt agtcactacc gccatcatag gcttcaaaaa
      661 caaaaacgag gcgtcgctct catcagcctc tacaacaaaa taagctgact ttccaagctg
      721 agcattcgca ccacaactat tcagctttcc gccaataaca aaactgggat ccaaaccacc
      781 ttcagcaagc aaactactga ccaagctcgt tgttgtcgtt ttgccatgag tgccggcaat
      841 tgctatacca tgcctaaatc gcattagctc agcaagcata gctgctctag gaataactgg
      901 aatcattaac tcacgagcag ccacaatttc aggattatta aaatcaacgg ccgaagacct
      961 tactacaaca tcagcgcctt taatgttttc cagcctgtga ccgatataga cttgaatacc
     1021 taatgacctt aagcgttgta ccgtgccact ttctccgaga tccgaaccag taatacggta
     1081 gccctggtta tgcaatactt cggcaatacc acacatacca gcaccaccaa tgccaacaaa
     1141 atgaatttgc tctacacgcc ccatgcgcgg agataaaaat tgctctgagt tattcaatat
     1201 tcccccttaa gccgttagcg gtctcaaccc aagagactgc caacgatttt catgatctat
     1261 gcgcaataat agtgcaataa cgatacaatt aattaccata ctggccccac cataactgag
     1321 taaaggcaga gttagccctt tggttggtaa cagtccggca ttaacaccca tattaataga
     1381 agcctgtaaa gctaaccaaa tagtcaaacc ataggctgta taggatgcaa aatgcctttc
     1441 ttgagtatag gcggtatatc ctatgtttaa tcctctaata accagtatac tatacaatgt
     1501 gatgacaact aaaattccaa ataaccctaa ttcttcagca atcacagcaa ataaaaaatc
     1561 agtatgtgcc tcaggtaaat agagtaattt ctgtatgctt tcacctaatc cggtaccaaa
     1621 ccatccacct cgtccgaaag caatcaatga ctgagtcaat tgataaccac tattgtattg
     1681 atctgcccac ggatccaaaa aggctgttaa gcgagcaacc ctataggggg aagataccgc
     1741 caacagagca agtgctgtca ctacaaccag cattaaacca aaatagtatc ttaatttaac
     1801 tccagctaaa aataacatag ccattactgt accagaaatg acaactgtcg ctccaaaatc
     1861 tggttcgagc agtaataaaa ctgaaacaac agccagtatt gccatgggct taatgaatcc
     1921 aaaaatgctt tcacaaaccg cctcttgctg gcgaaccaaa tacccggaaa gatagaatat
     1981 cattgcaagt ttcgttaatt ctgagacttg cacaccaatc ggacctaaag ctaaccaacg
     2041 tcgactgcca ttcactgatt taccaatacc cgggattaaa acaatgagca ataaaaacac
     2101 gcaaccaatc atcatgggca tgcttatttt ttcccagaaa ctgctgtctg tacgcaccac
     2161 aattaaggcc agcaagagtc ccacaaataa ataacaagcc tggcgaataa gaaaatgaaa
     2221 aggttgatga aagtactttg ttgaaatcat aaccgagctt gatgccacca tcatcaaacc
     2281 aattatcagc aaaccaaaaa ctgcccctat cagccattta tcataaagtg atatcggcct
     2341 gctaacgggt ttgcctcttt gatttaaatg tctcggtcgc atcgtgttca caatccgcgc
     2401 actgaagatg taaacacatc gcccctatga ttaaaatcac ggaacatatc caaactggca
     2461 caagctggag ataataaaac gacatcacca ggtttcgcac atgtcttggc aatagtcaca
     2521 gcgccctcca gagaagaagc acgcacgaca ggaactactt tggctaaagc cgattcaatc
     2581 ttgtcagcat cttcaccaat caaaacaatg gaacgaacaa attctgaaac tggctgggct
     2641 aattcttgga aatccgcccc ttttccctgt ccacccgcaa taagaacaat tttcccttgc
     2701 atagaacctc ctattccatt aatagcagag attgtagcac caatatttgt tcctttcgaa
     2761 tcattaatcc aacctactcc atctacttct cttacccact ggcatcgatg tggcagtcca
     2821 gggaaggttt ttaaaacatt aagaatgtgt tgcatagaaa ttcccgctgc ttcggctaaa
     2881 gcacaagcag caagcgcatt catccaatta tgcacccctt ttattaaaat cgattccact
     2941 ggcaaaagac gctccattcc tttcgccaaa taagtagtat tctcttgttc aatcagcccc
     3001 caatttccca ttgaaggcgc gtctttgcca aaagagatgc attttatatc tgcttgacag
     3061 gattggtgag gaacagtgta gacgtcttca cgattaaata aaacagcctt ggcaccacga
     3121 taaatccttt gttttgcttg cgtataggct tccatagtgt gatgcctgtc caaatgatca
     3181 ggcgttacat taagaatcgt cgcaaccact ggcgacaaag aataagttaa atccaattga
     3241 aaactggata actccaatac ccacaaatcg tagtgatgtt catcatccaa catatctaag
     3301 acaggagtgc cgatatttcc ggctacagct acgcgaaacc cggccgcctt agccatttct
     3361 cccaccaaag tcgttacagt ggatttacca ttagttcccg ttatggcaat gacaggggcg
     3421 ctaatctctc tggccaggca ttctatgtct ccataaattg atgcgcccgc ttgacgcgct
     3481 ctctctaaaa caggcgtatc cagtgccaaa ccggggctag tgataacatc cgtcacctgg
     3541 ctgattactt catcaggagt ctgttgcagg taaattggca catcaggaaa ttcattttgg
     3601 aattcagcta atccaggcgc ctctttacgt gtatcaaaaa caacaaacga tttattattt
     3661 ctcttcaggt atcttgctat agagagtccc gtcttaccca gccctgctac caaatataag
     3721 gaatgattca tgaatgcacc ttgtctatcg aagttttaaa gtagccaatc cgcataacac
     3781 aaatacaacc gtaataatcc aaaaccttac tatcactttg ggctctgacc acccctttaa
     3841 ttcaaaatgg tgatgtaatg gggccatcct gaataatctt tttccgcctg aatatttaaa
     3901 ataacccact tgtaatatga cagaaagcgt ctcaattaca aataatccac ccataatgag
     3961 caaaacaagt tcttgtctaa ctacaaccgc aactatccct aaagctgctc ctaacgccaa
     4021 agagcccaca tcgcccatga aaacttgggc aggataactg ttataccaaa gaaaacccag
     4081 gccggcacca acaatagaag aacaaaaaat cgttagctca ccagtattgg gcacataagg
     4141 aattcctaaa taattagagt atacggcgtt actcgatgca taggcaaaca cacccaaggc
     4201 accagccacc attactattg gcataatagc caagccatcc agcccatcag ttaaattcac
     4261 tgcattactg cttccaacaa tgacgaaata ggcaagtaca ggaaaaaaaa cgcccagatc
     4321 ccaagtgacc gttttaaaaa aaggaactgt gagctgagta tgtactggca aactggcatt
     4381 ccaatacaaa taagagacag caactaaagc aatgactgac tgccaaaaat atttccaacg
     4441 accaggtaat cctttgctat tttttaatac caacttgcga taatcatcaa cccaaccaac
     4501 caaaccatta gccaaggtaa ccaagagcac cagccacagg cttgtttgtc ttaaatcgca
     4561 ccagagcaag caactcacag tgatagccaa taaaatcaaa acgcccccca tagtgggagt
     4621 tcccgccttt gataaatggg tttgggggcc atcactccta accatttgac ctatttgcag
     4681 ccctcttaac cagcggatca ttaatgggcc acataacaaa cccactataa gggcagttaa
     4741 tgaggctaaa atagacctga atgtcaaata ttgaaaaact ctaaacgcat gatattgtcc
     4801 ctgcaataac tgcgttaacc aatagagcat aattattcct cttgttctta aaattatgct
     4861 caatgacttt tattgagcat gattccttta atggggcaaa aaagtggcca agcataccac
     4921 attcctgtcg attcaatgaa gaggttagcc ctctacctga accaaccggt gcacaacatt
     4981 ttccatagca ctggacctgg atccttttac caataccgtg gtatcaggtg ttaaatcatt
     5041 ttttaccact tcaatcaact cttcttgact gcaaaaatgc tgacctccct caccaaacgc
     5101 ctgggatgcc agttcactat gtaaaccgca actcatcaac ttatctattc ccagctggcg
     5161 agccgctacg ccaacttctc tgtgatgttg tgtcgtccat tcacccaact ctcccatatc
     5221 accgaataca aaaatttttc ttcctgggcg cccagataaa acttccaggg ctgttaagac
     5281 agagcgtaaa ttagcattgt atgtatcatc aataactgtt gattgatttt gccctgacaa
     5341 catcgtcatc cgacctttta caccaccaaa atgagccagt cctctttgaa tgtcattgcc
     5401 tgatattcct acagcataac aacaagctgc ggcagccaaa gcatttcgga cattgtgcaa
     5461 acctggtact tgtaactcaa tctccactct gctattaggt aaaaccaatg taaaatgagc
     5521 tcgcccatgg gtatccaagc gtatatcctg ggcgtaaata tctgcaggat gaagtgagga
     5581 aaaacgtaat atttttttat cagcaatcaa atcatcccaa aagtgagcgt aggcatcatc
     5641 atcattgact atagcaacac cagtcgggga taagccttga tggatttcac ctttggaacg
     5701 agcgactccg tctattgatc caaagccttc gacatgtgcc ggggcaatat tattaattaa
     5761 agacacatca ggctgcacta tccctaccgt atgtgctatt tctccgggat gattggctcc
     5821 caactcaaaa acagcatagc ggtgctgctt atttaaagct aacatgctta aaggaacccc
     5881 tatgtgatta tttaaattgc ctttcgtagc atgtgatggc tgcggtaaaa tagtcgctat
     5941 catctctttg acagtggttt ttccatttga ccctgttaaa gcaataatag ggcaactcat
     6001 ttcctgtcga tgtgccgcag caattttggc caaagccatt aaaggactgg caaccacgaa
     6061 ttgaggaata tgaacaccgt caatcgatcg attaactaca gcagctgaag cgcctttagc
     6121 ctcggcttct ttaataaaat catgaccatc aaagcgttcc cccactatcg caaaaaataa
     6181 atttcctggc tttaatgtgc gggtatctat actaacacca gtaatgtcgg cattaatctg
     6241 gcaagactga gaaagaagtg acgcaatagt aattaggttc atagtatttt gtgatgtcga
     6301 aaagactagt gtactaacac tcaggctcac agttcaagat ggtttgagaa tagatagata
     6361 agaatataac taaaaagtca agaattttaa gtttattaca atccgattca taagttatca
     6421 gacattaacc attaacagag tagataatga gcttcgcctg gtgcgaagcc ctatcctgaa
     6481 aatataaccg ctagatagtg caatgattct agcctatggg cattagtttg ttacacaatc
     6541 atccatcaaa tgctcaattt tcaagtatcg atgatatcgt gcgatacgct cctccgttag
     6601 aggtttgcgc tggtcatcca gcaaataagc gtcgagaccc tcacttaatt gttttgcagt
     6661 atctaacata acagaaaacc gttctgcatt aatctcagga ttatttgata tttgcatgaa
     6721 cagacataat cctcttacac tgaaagcacc gatattctgt aaatcaaaaa caccggttgc
     6781 tgtagctgca gctaaactgc aaagcactgg accttgtcca ttcgtgaatt gatgtctatg
     6841 aaacaagtgc ctctctccaa atctcaaacc agcagccaaa acagtttgca ataattcata
     6901 accggcaaat tgtcgatttt ccttggccaa caaaaacacc atggcggtgg aagaagaaca
     6961 gtcactatta ggttctgttc tttcatttaa ttcgcactct tcttcctcgg gaatcaactg
     7021 aggttgttct gtttttacag agggcttcct tattgcttgt cgcttttcag aaccatcaaa
     7081 attttcaaaa tccaaaggaa cctccctatt gaccttccgc acagcgataa tttcatcatt
     7141 atatgattga gagccatagg agttattttc tacttggcca agagaaggtt gatagtgttc
     7201 ttgatttaag ctctgtcttc tagtcttcat taaacggcca atagccacta gaacgccaat
     7261 taacaacagg acattaagaa ttaagctcca atttgcctgc ataagtttac tccatagtca
     7321 aagcttgttt cacatcaacc gcaaccaaac gagacactcc cggctcacgc atggtcaccc
     7381 caactaaatg ttcagccaat tccatagtta ccttattatg agtaataaac aggaattgta
     7441 tgaattgtga catctctttt actaaatcgc aaaaccgact tacatttaag tcatctaaag
     7501 gcgcatctac ttcatctaac atacaaaatg gtgaaggatt tagctgaaaa attgcaaaca
     7561 ccaaagccac agcagtcatt gccttttcac caccagataa tagatgaata gaactattac
     7621 gttttccagg cggctgcgcc attactacaa tccctgcttc taacagatta tcacaagtta
     7681 attcgagctg tgcccgacca ccgccaaata atcttggaaa aagcgcttta aaagaggtgt
     7741 tcacttcatc aaacgtattt tcaagacgcg atttcgtttc attatccatt ttttcaattg
     7801 cagtttccaa agtagataaa gcttggctca aatcattata ttgctcatcc aggaacactt
     7861 tgcgctgctg ttcggcagca aattcttcga tggcggccag gtttatagcg cctaaccttt
     7921 taattttctc caaaatcgct atcaattcgt cttcttttat actttgcgtg actccggatg
     7981 gaattcgctc taataaagac tgggcttgta aattagcctc atcaagagtt tcttgaattg
     8041 aactggcacg aacggatagg gcttgttttt ccatttggat ttgattcatg agttcctgaa
     8101 tccgttttac ttcctgatca tagtttaaaa ttaatttctc ttggtcttca agttgcaact
     8161 taagctgaga tactttttct cggctcaaag tcaacatcga ttcgatttca ccatgtttta
     8221 tcaataatgc tgccaattga tttttaaact cggcttctgg cgctactgac tgtaaataat
     8281 gttgagttaa ctcctctaaa cgcatctgtg cacgttgcaa acgctctttt tctctactta
     8341 tcctatcggt tatttgctgt atcttggtga cttctctatc atattccaac tcactttgat
     8401 gcaacaaagc acggacactc tctacttgtt tattttgttc aacgagaaca ctgttccatg
     8461 tgtctttttc atttataaat cgcttgaatt gcaactcaga agcttggtat tgaccttcca
     8521 gttctctgat tttatcagac atttccaatt gttcagcgtt tagctcttcc agcgacgact
     8581 ccagctctat tatctcgttc gtgatagcat ctttctgttt ttcagcatga aaaatagctt
     8641 gttcttgatt atttaccgca atgatatgat tgcgtagcgc ctcattactg gcatttaaat
     8701 ttaattgatg cagtttgata aaatttaaat tttcctgtag ccgcgcttga taactatctc
     8761 ttgctaaacg gatggattca attttttgtt gtaaagattc cacacttaaa ttcaactcga
     8821 caatcctttg ctgtctggca agaacgccta cctcatcttg ctctttttgg cgaggtaatt
     8881 taatccaccc cttgcctagc caaataccat tgacggttac aatagattca tgagccaata
     8941 aatcaggaaa ccacgccttt gcctcttcaa gattgtctgc ggcataaatg tgttcaagat
     9001 ggtttactgt tgagggaacc aagccttgaa ttttatcagc taaccttggg cgctcctgct
     9061 tttccccatt gagagctctc atcgtcacta cgctctcttt atgatcttcg caaaactgcc
     9121 accgagacca tagctcagca aaatcatcca atacataagc gattagatcg tcccctaata
     9181 cgatttcaca agaagattgc cattggggtt caacctttaa aacatccatt aatcttggct
     9241 tatctgacca ttccgcaatt ccgtccttat taggccgatt ttcctttttt gctgcaattt
     9301 gagcagcttt taatgcagca agctcagtgt ttaaacgatg aaattcatct tgcaatgcat
     9361 gcagttgttg ctcggtattt tgtaattgat tctgcaaatc cgattgagtc tcattcgctt
     9421 gcctaagctg ttcctcatca aactcctgtg tttctttcaa ttggaaatgc ttttcttgca
     9481 atttgatttt atttttttgc aaatcgtcaa gcgagatagc tgattgttcc aaattcagtt
     9541 tttccagcct taccaggact tgatggcgct tctgctctaa atgttgtagt ttggcttgag
     9601 tgagttggta ttcattttta atggtattgg aattaatttg cacctcttgc caaattttct
     9661 cccattgcga ttgttccttt tgtttttctt gccatagaga ttcctgttct ttgaattcca
     9721 tttttaaatg atgcaaatgc tcctctaact gttgagcctt aagcttgcaa ttgtgcaatt
     9781 cttcattatc cagcttaagt tgctcttcaa tgagttgaga atcaacctgc atttgctgcc
     9841 tgtcattttc caaacgcttt ttttctcttt cttgttgctg aatggcttct tcaagacgag
     9901 ctatctcagt tcctgaatga tagagagaat cctgaattga ttgggattga tcaatggcat
     9961 cttgtacttg ttcatttaaa actgcccgct ccttgtaaca actagttaat aaactttgtt
    10021 gctgctcatt gcggattgct aactcttgta attctcgctg ttttaccgtt tgtttttctg
    10081 agtattcctg ccatttaaga gcctggatct cagctcggca aattttttcc tcttccttta
    10141 aaatggtata gcgttctgct gctttggcct gccgctccaa acgttgcaaa tgtttgccca
    10201 attcttctct aatatcagcg acgcgcgtca ggttatccct tgtttgttca atacgttgta
    10261 aagtttcccg gcgacgctct ttgtattttg aaactccagc cgcctcctcc aaatagactc
    10321 ttaattcttc gggttttgct tctattaatc gagatacagt accttggcca ataattgagt
    10381 aacttcgtgc ccctgctcct gtgcctaaaa aaatatcagt aatatctttt cgcctgcatc
    10441 gagctccatt caaataataa aaagattctc catcccgagt gactattcgc tttacagcaa
    10501 tttcaccata actggcaaaa gggccagtca gtcttcccag gctattatca aataccagtt
    10561 ctactgacgc ctgtcctacc ggtttgcgat tggaagaacc gttaaagata atatccgtca
    10621 tggactctcc acgaagattt ttggcagagc tttcgcccat aacccagcga acagcatcaa
    10681 taatattgga cttgccacat ccattcgggc ccacaacagc gaccaaacga ctgggaaaat
    10741 gaaccgtggt gggatcaaca aatgatttaa aaccagccaa ttttaattgc ttcaaatgca
    10801 ttttaactgc gaattataaa ataataatcg gttagtatat cgtaggaagt gaatcctgac
    10861 tatttcagaa catccatatc gctctcttct gccatcgtta aggacaacat gctcaattga
    10921 caaacaattt tatcgcttta taatacatac ttctgttaac aaatgtaggt aaacaatgaa
    10981 acagcaattc tataaattgc cccaaattct aataagcctt tgtctcctat tgattctttc
    11041 caccccaatc caagcagaac cgccaaatct tagtttaatt agaaaagaaa t
//
LOCUS       DACTJE010000104        11022 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.104, whole genome shotgun sequence.
ACCESSION   DACTJE010000104 DACTJE010000000
VERSION     DACTJE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11022
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.104"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            190..804
                     /gene="mavI"
                     /locus_tag="I9760_002042"
     CDS             190..804
                     /gene="mavI"
                     /locus_tag="I9760_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavI"
                     /protein_id="HAT5043805.1"
                     /translation="MSSVKLCFALFRLYCYWRKLSMGLSAQQKTAIKSYFESNYNLFF
                     GIPTERKEGDLTPNEHVMVLQYLRFYLLKLPIECLEEELVNKQINGDDTVGILLEDFL
                     GNTLSPMNPDAYFHSQKNFIQMGNAEDPAKPEDIIQYLIEVIENMSQQMDHGYLKKES
                     DPRIEKSNSSYASKALKWGLFGGAALAITYAVSKLSDKSHTNSL"
     gene            1031..1588
                     /locus_tag="I9760_002043"
     CDS             1031..1588
                     /locus_tag="I9760_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2443 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043806.1"
                     /translation="MIRKSEMIRHFFIVLFFFVGFMNQASALPWFENENDLRNNNKLR
                     YRAHDEIKKNQIDFSGRWSGQCDSNPAVDLTIKHTREHISISYGFMEEKYIPGEVRAE
                     SSSRLSLSESGSTTVKWNQDNTALIFINYHLFINQSGSLNVFFSKVSMALEQEQLVVK
                     GQHFQTGNTLDDIKQETISCIYDRK"
     gene            complement(1712..2122)
                     /locus_tag="I9760_002044"
     CDS             complement(1712..2122)
                     /locus_tag="I9760_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAT5043807.1"
                     /translation="MKPLIPYLLFNGTCREAMTFYQNCFGGELELMTYGDSPEGACDK
                     KADKNDIMHACLRQGGFTLMASDWPGGEAKQGNNVHLNIDCSTKSEIEALFKKLSQEG
                     TVVQALADTFWGAHFGMLIDRYNIHWMLNYSLKQ"
     gene            complement(2223..2696)
                     /locus_tag="I9760_002045"
     CDS             complement(2223..2696)
                     /locus_tag="I9760_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAT5043808.1"
                     /translation="MQKITPCLWFNDKAEEAVTYYISIFKNGSIEKITYYGKEGVEFH
                     KKPAGSVMTILFNLNGQQYVALNGGPEFRFNESISLMVNCDTQDELDYFWKELSADGE
                     EGPCGWLKDKFGLSWQIVPDILAELLSDHQKSEKVMRVLLQMKKLDIAKLKAAAE"
     gene            complement(2734..3693)
                     /locus_tag="I9760_002046"
     CDS             complement(2734..3693)
                     /locus_tag="I9760_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAT5043809.1"
                     /translation="MGILIDGIWHDENNINTDKEGRFVRAESGFRQHITANGESGFKA
                     EPDRYHLYLSYACPWACRTLIMRKLKGLEKIISVSFSDPVMGKNGWSYGESGNETADH
                     INHIHYLKELYLVARKDYTGRVTVPVLWDKKKKTIVNNESSEIMRMLNSEFNAFSSTD
                     HDYYPKQWRQKIDEINQLVYENINNGVYKCGFAKSQKAYEEAFDALFNTLNQIENLLA
                     TQRYLLGEQLTEADWRLFTTLIRFDAVYHGHFKCNLQRIADYPHLSNYLRELYHFPGI
                     KETVNFNHIKQHYYRSHLSINPTGIVPKGPKLNLDEPHNRYEF"
     gene            3842..4393
                     /locus_tag="I9760_002047"
     CDS             3842..4393
                     /locus_tag="I9760_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAT5043810.1"
                     /translation="MFNVAVIVGSLRKESFNRKLAFALDKLQHPTFQLSHIKIDDIPL
                     YNQDFDNNLPDSVIRLKKEITEADAVLFVTPEYNRSIPGVLKNIIDWGTRPYGKNVWA
                     GKLMAAIGTSPGAIGTAVAQSHLRSIMVAIDGVCFGQPEIYLVYKDGLIDNNFSITME
                     STRNFLQGFLDRFAREIEKHKSI"
     gene            4660..5850
                     /locus_tag="I9760_002048"
     CDS             4660..5850
                     /locus_tag="I9760_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HAT5043811.1"
                     /translation="MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRYFNTDS
                     TTMQATLYLFMLTVALGQLLVGPLADRFGRRQMALSTALLFFAGSLISALAPSVPALI
                     FARIIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDM
                     TQDWHSGFYFLTLLGLLMTGIAFRYIPDYHYPKPELLQMRVHQVMRQIISNSDFRQYT
                     WVASASLLGLYLFCAQSPGILITQLYLSATEYGFWFGLNAMTVFVANMMAARLTYSHS
                     LESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIGISMGTATALALKDF
                     EQQAGVATALLGACQFGLAGLIGVLTAQWMPGPLSVAIPVLCFTAVGLARITKFSFRI
                     LSGS"
     gene            5911..6102
                     /locus_tag="I9760_002049"
     CDS             5911..6102
                     /locus_tag="I9760_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043812.1"
                     /translation="MTFSVVYPGCGSVLSFSGNFTKKGNIIDTISILTHQAQDITHEG
                     PGARFIGHDRFKKVAASNT"
     gene            complement(6209..6589)
                     /locus_tag="I9760_002050"
     CDS             complement(6209..6589)
                     /locus_tag="I9760_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043813.1"
                     /translation="MTRNSVKTVCIFLSIILYVIALCLPAAIIPDFERGIHQTHYGFE
                     ILITGWMGFLAWQPAWLANLLYLFSLLAYGSKNSYRFGITAFIFAMLSPFILVEKLLI
                     GFYVWLASFAILLYGNNLHEMTIE"
     gene            complement(6626..7669)
                     /locus_tag="I9760_002051"
     CDS             complement(6626..7669)
                     /locus_tag="I9760_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HAT5043814.1"
                     /translation="MITNNIRFNWIIFILLISLLNLANINELHADEAGEQSQFNLESY
                     SPQTKKLCQTVAHLSAPGQNQLDEASKASLKNCDALDLYYGFNTAPDYDKAFQCALMN
                     KDYNVLVMAYANGRGVKFNPDLAMHFACMMEDAAPAEMDGRIAHLAQIKQGNKKTSFD
                     ICDDITSGYMMGWCSSIDQRLQDAKRNKKINALVSQWTTQEQLLYQQVRKTAEVYIHD
                     HSMNEIDLSGTARSAFAINAQLGLNQSLFELLQKVDRCEAPLVTTKQYEELDRQLNSI
                     YKKLMADTTSFQYTTITKEGIKKTEIAWIKYRDAWIQLARVKCPKISAESWQALLIQD
                     RIKLLNEILELAE"
     gene            complement(7696..8190)
                     /locus_tag="I9760_002052"
     CDS             complement(7696..8190)
                     /locus_tag="I9760_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2434 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043815.1"
                     /translation="MTIMMNKDKHSLPAIAAEEIPPRTKPSIYPEPFASMMAGRQKHA
                     LGDFFGIKNFGVNLTRLAPGAQSALLHKHKLQEEFIFILKGQPTLVTETADIQLHPGM
                     CAGFTPNGEAHQLVNRTADEVVYLEIGDRTQGDEVNYPADDLAAVFGDDGQWHFMHKN
                     GMPY"
     gene            complement(8457..10217)
                     /gene="ceg30"
                     /locus_tag="I9760_002053"
     CDS             complement(8457..10217)
                     /gene="ceg30"
                     /locus_tag="I9760_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg30"
                     /protein_id="HAT5043816.1"
                     /translation="MDIHMTPLFPEKNTPLTIKQGPVDDCYLLVALDCIFNSGDEARQ
                     LLASKFTQTLLGLTVRIKRNNQSTRLDSQKMRGKYIHVYDRNTDEDVFFIGQQKLKEI
                     DNSKKGVETNSLAIKVLERISSYYYTGDWDHTNDSGSLTAHSIRQRHMETSTRFVGKL
                     LGIHAEDTEEMDAIIKLKLMNPKEAVYISFAYGEPDANGKIYDRHALRIDKVVPKING
                     GYDFILVNPWDNQKQETFSLEELEKRDCRFCTFSLNQQRHEITRILLKNPVEQGQYIF
                     ANPELFTLLLQMQKLSLLSDAKNIQACINLHKQMPYLPSLYNSLTSDEQKKLIQGMVG
                     TKGDKAKFIKHLIINTPRLDLIKTIFQKEPLENKTENMIVDLALEAKKKHSPARIVFE
                     DPEFFTLIMNRAIHHKATIRGCSQEQAKKLIESKIINFYFDNLGTDSLTRNARLCELF
                     AAKIFTKTSIESWFAARLSLTNVDLSQALRTLGFSDQRSGVMSAPVLKKQTALVAGQV
                     GFFPIHKSSPALVTNKTPAPIQELTAKKSSVPTLPSIVKKPSTPTPALLGNGLSIYTP
                     EKPSVKTLPPLKGVRLTSLP"
     gene            complement(10354..11013)
                     /locus_tag="I9760_002054"
     CDS             complement(10354..11013)
                     /locus_tag="I9760_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HAT5043817.1"
                     /translation="MGKYYHEYIGNYQGKYGKGSDVNRFFPTLGNHDWLARKTCLYQG
                     TLPYFSYFTLPGNQSYYDFVRGPIHFFALDSDSHEPDGSKEGSKQYQWLFEQVQQSKA
                     PFKIVYFHHAPLSSGKHSSNTSMQWNFAAMGIDVVMGGHDHHYERIERNGIVYYVNGA
                     GGAELYSYKKWVEGSKFFYSKHHGFMLIIALEHALKIQFINDDDEIKDEIVIQEKPGV
                     G"
BASE COUNT         3175 a         2028 c         2115 g         3704 t
ORIGIN      
        1 aggttatcaa tagaagcaac aaaaaacgaa tgaagcgatt catattgatt cgtggaaccc
       61 aaagaaaaga ttaacttaac aaatccctcc agacaatgac agcaaattct taatgttgtt
      121 tttatttgat tatttataat ttgcacataa aattcattgt aaggtcatca tgaaagattt
      181 aagatcactc tgtcttctgt taagctttgc tttgcattat ttagacttta ctgctattgg
      241 aggaagctca gtatggggtt atcagctcag caaaaaacgg ccataaaatc ttacttcgaa
      301 tccaattata atctgttttt tgggattcca actgaacgta aagaaggcga tttaacaccc
      361 aatgaacatg tgatggtatt gcaatacctg cgattttatt tattaaagtt gcctattgag
      421 tgtttggaag aagagttggt gaacaaacaa atcaatggtg atgatacggt aggtatttta
      481 ttagaggatt ttctgggcaa cactctttct ccaatgaacc cggatgctta ttttcatagt
      541 caaaaaaatt ttatccagat gggtaatgct gaagatcctg caaagccaga agatattatt
      601 cagtatttaa tagaagtcat tgagaatatg tcgcaacaaa tggatcatgg ttatttaaaa
      661 aaagaaagtg atcccagaat agaaaagagc aacagttctt atgcgtcgaa agcactaaag
      721 tggggcctat ttggaggagc tgctttagct ataacatatg ctgtcagtaa attgtctgat
      781 aaaagccata ccaatagtct atgaaagcga caatattcgg tttcggggta acggaatatc
      841 aatttccagc ggatttctcg aagtacgtct tataccaaag caaaaccatg ctatgttgta
      901 agagttgtgt caaaagcaag aaccctgaat tgctattttc ccggatactc cttaaaaagt
      961 agattgcaat gatattataa attcgttttc tgcgagacag aaaaagtatt attctgtaca
     1021 aacctgccta ctgataagga agtcagaaat gataagacat ttttttatag ttttattctt
     1081 tttcgtcggt tttatgaatc aggcttcagc tcttccctgg tttgagaatg agaatgattt
     1141 aagaaataat aacaaactga gatatagggc tcatgatgaa atcaaaaaaa atcaaataga
     1201 tttttctggc aggtggagcg ggcaatgtga tagtaatccg gctgtggacc taactatcaa
     1261 gcatactcga gaacacattt ctatttccta tggatttatg gaagaaaaat atattccggg
     1321 tgaagttcga gcagagtcat cctcacgctt atctttatct gaaagcggca gcactacagt
     1381 gaaatggaat caagataaca cagctttaat ttttattaat tatcatttgt ttatcaatca
     1441 aagcggtagt ttgaatgtgt tttttagtaa ggtttccatg gcattggagc aagagcaact
     1501 ggtagtcaaa ggacagcatt ttcaaacagg aaataccttg gatgatatta aacaagaaac
     1561 tatctcatgt atttatgacc gtaaataatg acataaagta aatccctgta tgatgagctt
     1621 tttgctggtc acatgggact tactttactg gttagtgaaa cgatctcaat tgattaaaaa
     1681 ttgtcgatca gacttttttg tattttgatt tctattgttt caaagaataa tttaacatcc
     1741 agtgtatatt atacctatca atcagcatac caaagtgcgc gccccaaaat gtatcggcga
     1801 gtgcttgcac taccgttcct tcctgactta gttttttaaa cagggcttca atttctgatt
     1861 ttgtgctgca atcaatgttg agatgcacat tattgccttg ctttgcttcg ccacctggcc
     1921 aatcagatgc catcagagtg aaaccacctt gtcttaaaca agcatgcatg atgtcgtttt
     1981 tatcggcttt tttatcacaa gccccttcag gggaatcacc atacgtcatt agttctaatt
     2041 ctcctccaaa acaattctga taaaatgtca ttgcttctcg acaggtaccg ttgaaaagaa
     2101 ggtaaggtat tagcggtttc atgtattctc cttgataaat tgattattgc aagactcgta
     2161 actcccaatt tctttgctaa aggtcattta ttgcttcgat tttatgattt ttccttagca
     2221 tcttattctg ctgcagcttt cagctttgcg atatccagtt ttttcatttg cagaagcact
     2281 ctcatgactt tttctgactt ttgatggtca ctcagcaatt cagccaagat atcgggaact
     2341 atctgccaag ataatccaaa tttgtctttg agccagccac aaggaccttc ttctccatct
     2401 gctgagagtt ccttccagaa gtaatccaac tcgtcttgtg tatcgcaatt gaccatcagt
     2461 gagatggatt cgttaaatcg aaattcaggg ccgccattaa gtgccacgta ttgttgtcca
     2521 ttcaaattga agaggattgt catcacactg cctgctggtt ttttatggaa ttcaactcct
     2581 tctttgccat agtaagtaat tttttcaata ctcccatttt tgaaaatcga gatataatac
     2641 gtcactgctt cttcggcttt atcattgaac catagacaag gagtgatttt ttgcatggtt
     2701 ttttatccta atttctagct attgaaaaat ttattagaat tcatagcggt tatgtggttc
     2761 atccaaattt aattttgggc ccttgggaac aattcccgta ggattaatgc ttaaatgact
     2821 tcgataataa tgttgtttga tatgattaaa atttacggtt tctttgatac caggaaaatg
     2881 ataaagctct cgtaaataat tggataaatg cggatagtcg gcaatccgct gcaagttgca
     2941 tttgaagtga ccatgataca cggcatcgaa ccgaattaat gttgtaaata aacgccagtc
     3001 ggcttctgtt agttgctctc ctagcaaata tcgctgtgtt gccaataggt tttctatttg
     3061 atttaaggta ttaaataacg catcaaatgc ttcttcataa gctttttggg atttagcgaa
     3121 gccgcatttg taaacaccgt tgttaatatt ttcatagaca agctgattaa tctcatcaat
     3181 tttttggcgc cattgttttg gataatagtc atgatctgta gaactaaagg cattaaattc
     3241 gctattcaac atgcgcatga tttctgatga ttcattattg actatcgttt tctttttttt
     3301 gtcccataaa acaggaactg ttactctacc ggtataatct tttctggcaa ccaaatacaa
     3361 ctctttgaga taatggatat gattgatgtg atcggctgtt tcattacctg attcgccgta
     3421 actccagcca tttttaccca tgactggatc tgaaaaagaa acagaaataa ttttttccaa
     3481 tcctttcagt tttctcatga ttaacgttcg gcatgcccag ggacaagcat aggacaggta
     3541 taaatgatat cgatcaggct ctgctttaaa cccactctct ccattcgcag taatgtgctg
     3601 acggaaacct gattcagcac gaacaaaacg tccttctttg tctgtgttta tattgttttc
     3661 atcgtgccaa ataccatcaa ttagtatccc cattattaat tccttatata tcaacaactt
     3721 attaatattt tttgaatact gacgcagcat gaatacgtct tgtttaataa ctgcttttta
     3781 atgcataatc attgataaat cgattaattc attttgtatc aataaattta aggaaaaatt
     3841 tatgttcaat gtagctgtta ttgtcggaag ccttcgtaaa gaatctttta atcgtaaatt
     3901 agcgtttgct ctggacaagc ttcagcatcc tacatttcag ctatctcata ttaaaataga
     3961 tgatattcct ctttataacc aggattttga taataattta cctgactcag tcattcggtt
     4021 aaaaaaagaa attactgagg cagatgctgt tttatttgtg acacctgaat ataatcgttc
     4081 gatacccggt gtattgaaga atattataga ttgggggacc aggccatacg gaaaaaatgt
     4141 atgggcaggc aaactcatgg cagctattgg aacgagtccg ggagctattg gaaccgctgt
     4201 tgctcaatcg catttgcgaa gcataatggt tgcaattgat ggcgtttgct ttgggcagcc
     4261 tgaaatttat cttgtttata aagatggact gattgataac aatttttcta ttaccatgga
     4321 gtctacccga aattttctgc aaggatttct ggatcgtttt gccagggaaa ttgaaaaaca
     4381 taaatcaata taagtatgtc gactggttac ccaaagcaat gtttctggca attggtgcat
     4441 ttttctatgt tcccgatcat tcattttggc taaagcctct tgctcacttc gttattcctt
     4501 atgttttacg gtagtccaga atttttgcgt ttggactacc aataattaat tgaataacat
     4561 ctgttgaatc agcaatttta taacattgag tgtaaatctg ttacaattaa gcgcagttta
     4621 tatttttcat aagtaatatt ttatcaggaa caccattaat tgaattttat tggctggaca
     4681 gaaagacaaa gtctttttcg attatttcca ctcattatca gcatttcttt tgctatggat
     4741 gtttttgtgc ccgcaattcc ggaaatgagt cgttatttca atactgacag tacaacgatg
     4801 caagctaccc tttatttgtt tatgctcact gtagctttgg gtcagctatt ggttgggcct
     4861 ttggccgatc gttttggacg aagacagatg gccttgagta cggcacttct tttttttgca
     4921 ggatcgttga tctctgcgtt agctccgtca gtaccggcat tgatttttgc acgtatcatt
     4981 caggcagctg gtgcctgtgg aacctattta ctatgcttta ttatcgttcg agataatttt
     5041 tcaacgggca cctgtgcccg cttgtttagt atcctgtctg ggacgaattc gatggttgcc
     5101 agttctgctc cagttattgg gggattattg ttggatatga cgcaagattg gcatagtgga
     5161 ttctactttt taaccttgct tgggttatta atgactggca tagccttccg ttacatccct
     5221 gattatcact atcccaaacc cgagctgctc cagatgaggg tgcaccaagt gatgaggcag
     5281 ataataagta actctgactt tcgccaatac acatgggttg cctcagccag tttgctggga
     5341 ttgtacttgt tttgtgctca atcaccaggt attttgatca cacaactgta cctgagtgcg
     5401 actgaatatg gtttctggtt tggtttgaat gcaatgactg tgtttgttgc caatatgatg
     5461 gccgcacgac ttacttattc tcactcattg gagagcatag tgcgttgggg cttggtttta
     5521 atgacactgt catgcttttt tatgctggct cttaatcttc gtcaatgcag cattttaagt
     5581 tttatgcttc ccatgctttg cttgactgtg ggtattggta ttagcatggg cacagcaaca
     5641 gcgcttgctt taaaggattt tgaacagcaa gctggcgtcg ctaccgctct gttgggtgct
     5701 tgccagtttg gtctcgcggg cctgattggt gttttgacag ctcaatggat gcccgggcca
     5761 ttaagtgtag ctatccccgt actttgtttc actgcagtgg gattggctag aatcaccaaa
     5821 ttcagtttta gaatattgag cggctcttaa tcttgtcctc aggcaattgg ccaaaaaaga
     5881 ccgattatag gttatgctgt aggtaacact gtgacattta gtgttgttta tcccgggtgt
     5941 ggttctgtat taagttttag tggtaatttc actaaaaaag gaaacatcat tgataccatt
     6001 tccatcttga cccatcaggc tcaggatatt actcatgaag gaccgggagc tcgtttcatt
     6061 ggacatgatc gctttaaaaa agtagctgca tccaatacat agaatgattt ttccaagcaa
     6121 gcttacaata agcctcatgc caagacatga ggctttaatg attttgtaga tgttctatag
     6181 acaaggaata gacttattaa tcttaaatct actctatagt catttcatgc aaattattcc
     6241 cgtatagtaa gatagcaaaa ctggcaagcc atacgtaaaa tccgattaat aatttttcta
     6301 caagaatgaa tggcgaaagc atggcaaaaa taaaagctgt aatgccaaag cgataactgt
     6361 ttttggatcc ataggccagt aatgaaaata gatacaaaag gtttgccagc caagcaggtt
     6421 gccatgctag aaaacccatc catcctgtga ttaagatttc aaatccataa tgtgtttgat
     6481 gtatccctcg ttcaaaatca ggtattattg ccgcgggtaa gcacaaggca atgacataaa
     6541 ggattatcga taaaaagata catactgttt tcactgaatt tcgagtcatt gattactcgc
     6601 tttatttgat agtcaataac attaattatt cagctaactc aagaatttca ttgagcaatt
     6661 taatgcggtc ttgaattaag agcgcttgcc agctttctgc gctgatcttg gggcatttca
     6721 cacgagccag ttgaatccag gcatccctgt atttaatcca ggctatctcg gtttttttaa
     6781 taccttcttt ggtaatagta gtgtattgaa aggaggtagt atctgccatc aactttttat
     6841 agatactatt caattgccta tccagttcct catattgctt tgttgtcaca agaggagcct
     6901 cacatcgatc taccttttgt aacagctcaa acaggctttg attgagacct aattgcgcat
     6961 tgatggcaaa tgcgcttctg gctgttcctg ataaatcaat ttcattcatc gaatgatcat
     7021 ggatatacac ttcggcagtt tttctaacct gctggtataa tagttgttct tgagtagtcc
     7081 attggctgac tagggcatta atttttttat tgcgttttgc atcttgcaaa cgctgatcaa
     7141 tagaagagca ccaacccatc atatacccac tggtaatgtc atcacaaata tcaaaagaag
     7201 ttttcttatt accctgtttt atctgtgcga gatgggcaat acgaccatcc atttccgctg
     7261 gagcggcatc ctccatcatg caggcaaaat gcatggcaag atcaggatta aattttactc
     7321 ctctaccatt ggcgtaagcc ataaccagga cattataatc tttattcatt aatgcacatt
     7381 gaaaggcttt gtcataatcg ggggcggtat taaaaccata atagagatca agcgcatcac
     7441 agttttttag tgaggcttta ctggcttcat caagctggtt ttggcctggt gctgataaat
     7501 gagctactgt ttggcataat ttctttgttt ggggagaata actctctaaa ttaaattgac
     7561 tttgctctcc agcttcatct gcatgaagtt cattaatatt ggctaaattt aataaactaa
     7621 ttaatagaat aaagataatc caattaaatc ttatgttgtt agttatcata attttttctt
     7681 tctaattatt tgttattaat acggcattcc atttttatgc ataaaatgcc actgcccatc
     7741 atcaccaaac actgccgcaa gatcatccgc aggataattt acttcgtcgc cctgggttcg
     7801 atctcctatt tcaagataaa ccacttcatc agctgtccga ttgaccagtt gatgtgcttc
     7861 gccatttgga gtaaatccag cacacattcc gggatgtaat tgaatgtcag cagtttcagt
     7921 gactaatgtt ggttgccctt tcaggataaa aatgaattct tcctgtagtt tatgtttatg
     7981 cagcaaagcg gattgtgccc caggagctaa tcttgttaag ttgacgccaa aattttttat
     8041 tccaaaaaaa tcccctaatg catgtttttg acgaccagcc atcatggatg cgaatggctc
     8101 aggataaata gagggtttgg ttcgcggggg gatctcttct gctgctatag caggcaatga
     8161 atgtttgtct ttgttcatca tgatcgtcaa tttgagttct atccttgcaa tatttaatca
     8221 atattagcat aaagtaagtt atcttgaatc cctactttca ggaattctaa attatggatt
     8281 tgttactgaa tttagcgaaa ccaaagcaag attaatctta ggcagcagaa agcaaagtat
     8341 atagaaagat tttgagtgag ttagattttt ctatgaatct gtttccagat ctttgcttta
     8401 ggagtttcag gttaaaaaat taatttccag ccagcacaat agatatatta tgaattctag
     8461 gggagtgatg taaggcgcac tccttttaga ggagggagcg tttttacaga gggtttctct
     8521 ggggtataaa tggataatcc gttccctaga agtgctggag ttggtgtaga aggttttttt
     8581 actatagatg gtagggtagg aacagatgat ttttttgcag tcagttcctg gattggagcg
     8641 ggagttttat ttgttaccag cgccggtgag gatttatgga ttgggaaaaa tccaacctgg
     8701 ccagccacta aagctgtttg cttttttaga accggtgcgc tcattactcc agaacgttga
     8761 tcagaaaatc ccagagttcg taatgcttga gacaaatcaa cattagtcaa agaaagtctg
     8821 gcagcaaacc aggattcaat ggatgttttt gtgaaaatct ttgcagcaaa taattcacat
     8881 agccttgcat ttcgagtcag actatctgtt cccaggttat caaaataaaa attaattatt
     8941 ttggattcaa tcaatttttt tgcttgttcc tgactgcatc cccgaatggt tgctttatga
     9001 tgaatcgctc gattcatgat taaagtaaaa aattccggat cttcgaaaac aatgcgtgct
     9061 ggtgaatgtt tcttttttgc ttcaagtgcc aggtcaacaa tcatgttctc agttttattt
     9121 tcaagaggtt ctttttgaaa aattgtttta atcaaatcca gtcgaggggt gttaattatc
     9181 aggtgtttta taaattttgc tttatcaccc tttgtgccta ccattccttg aatcagtttt
     9241 ttttgttcgt cagatgttaa tgaattgtat agggagggca agtaaggcat ttgtttgtga
     9301 agattaatac acgcttgaat gtttttagca tcggataaca ggcttagctt ttgcatttgc
     9361 aagagcaggg taaacaattc cgggtttgca aaaatatatt gcccttgctc taccggattt
     9421 tttaataaaa tacgtgtaat ttcatgtctc tgttgattga gactgaaggt acaaaaacgg
     9481 caatctcgtt tttcaagttc ttctagactg aatgtttcct gtttttgatt atcccatgga
     9541 ttgactaaaa taaaatcata accaccattt attttaggga ccactttatc tattcttaac
     9601 gcatgacggt cataaatttt tccattggca tctggctcac cataggcgaa gctgatataa
     9661 acggcttctt tggggttcat taatttgagt ttgatgattg catccatttc ttcagtatct
     9721 tcagcatgaa tacctaataa tttgccgaca aaacgggtgg aggtttccat gtgtctttgt
     9781 cttatgctat gagctgtcag acttccagag tcattggtat gatcccaatc tccagtgtaa
     9841 taataggagc tgattcgttc taaaacttta atagcgagtg aattggtttc tacccctttt
     9901 tttgaattat caatttcttt gagtttttgt tggcctataa aaaaaacatc ttcatctgta
     9961 tttcgatcat aaacgtggat atatttgcca cgcattttct gagaatccag tcgggtactc
    10021 tgattatttc gtttaatgcg gacggttagc ccaagtagag tttgcgtaaa ctttgatgct
    10081 aacaattgac gagcttcgtc tccagagtta aaaatacaat ctaatgcgac tagcaggtaa
    10141 caatcatcga ctgggccttg ttttatagta aggggagtat ttttttctgg aaacaaaggc
    10201 gtcatatgga tgtccaataa tacaaaaagt atttaatctt gccattttat caaagaaata
    10261 ttatgtgaat attaaggaaa gctgaggcaa gcatggtgta aacccatcat tttcttatgt
    10321 tcaggactta caaaaaccct caaggtcctg atgttatcca acacccggtt tttcttgaat
    10381 gactatttca tccttgattt catcatcatc attgatgaat tgaattttca gggcatgctc
    10441 gagagctatt atcagcatga atccatggtg tttagagtag aaaaatttgc ttccttccac
    10501 ccatttttta taggaataaa gctctgctcc tccggctcca ttgacataat aaacaattcc
    10561 gttgcgttcg atacgctcgt agtgatgatc atgacctccc atgaccacat caatacccat
    10621 agcggcaaaa ttccattgca tactggtatt ggaactatgt ttgccagaac tcaacggagc
    10681 atgatgaaaa tagactattt taaagggagc ttttgattgt tgtacctgtt caaacaacca
    10741 ttgatattgc ttcgaacctt ccttgcttcc atccggttca tgagaatcac tatccaaggc
    10801 aaaaaaatgg attgggccgc gaacaaaatc ataataactt tgatttccag gaagggtaaa
    10861 atagctgaaa tagggtaatg ttccctgata taaacaagtc tttcgagcaa gccaatcatg
    10921 attacccaga gtagggaaaa agcgattgac atcagaacct ttcccatatt ttccctgata
    10981 attgcctatg tattcgtgat aatatttacc cacattctta tc
//
LOCUS       DACTJE010000105        10875 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.105, whole genome shotgun sequence.
ACCESSION   DACTJE010000105 DACTJE010000000
VERSION     DACTJE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10875
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.105"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..219
                     /locus_tag="I9760_002055"
     CDS             <1..219
                     /locus_tag="I9760_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043818.1"
                     /translation="DQQNQDKQNKAQQNKVQNKKNDKQEGEIQSEANQEKQLAKEQWL
                     RLIPDDPGGLMREKFLRDHLRRERGWYQ"
     gene            216..1865
                     /locus_tag="I9760_002056"
     CDS             216..1865
                     /locus_tag="I9760_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein BatD"
                     /protein_id="HAT5043819.1"
                     /translation="MKKLMLWGLLSLFSSLSFAEIKVELEPSQVTMGETFKLSITQED
                     QQGGGVPDLTELQKDFVILGTERHVNYSIINGQAQSQSQWIVLLRALKPGIFTIPPIK
                     VGLEQSLPITINVQGSANTQALPDEAIQKQDVFLTAEVNEKNPYVNQQIIYTVKLYNS
                     KRLLDADYQGPKVDDALLIPLGDAKRYHAVHNNINYVVEEQTYAIYPQKSGTLKIISP
                     IFNALVYDYNPQRTKATDKAINLTIKPIPKKYQGKLWLPAKQVKLSEQYENSNQTLSQ
                     GSTLTRTVTLEGIGIPAQLLPTLTFQETDFFSVYPEKGAERNQVKQGELIGSVEIKVT
                     YLFNKAGKTTIPELRLTWFNTETGKEEIAVLPPRSIDIIPSTAVKANTANTQVTGKEM
                     SASSQLAPDSASVANESGGWAVKLAIFFGLAWLITLSLWIWQKRPRESSSKKEYKKAL
                     DALNKACSQCNPKKARDAVLKWASLQWPDAPMLNLTDLAQLVRDAHLRKQLHLLSQVL
                     YRSQEKTLWRGDELLRAVQAVKRNESVKKRKNSNLPPINPF"
     gene            2102..3484
                     /locus_tag="I9760_002057"
     CDS             2102..3484
                     /locus_tag="I9760_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAT5043820.1"
                     /translation="MHVNEYIHYDVQELAELIKNHQVGAEEVLRCAQERLYQVNPSLN
                     AVVTDCFEYAHQCLAKMKGNEPYYGVPLLVKDLGHAIEGIRLTEGSRFFSNNLAETNS
                     DLINKLIALGFVPFAKTNTSELGLSCVTDSNLFGPCRNPFDTMRTAGGSSGGSAAAIM
                     AGIAPVATASDGGGSIRIPAACCGLFGFKPTPGLVPSGPLVDEAWSGMATSFILTRSM
                     SDAIALFKNVINPMRLNRLSRKKKKLLITELTGAFAEVPIARECQEAVEKIKEILKNS
                     GHRLNEVHLPLDLQVISSCAHTLIAASTYAKIKTQEQISGRKPMIHELEPITWEFYYR
                     GKMVSAYEFIIAKNKLYQSLRPLHQLLEKTDIVLTPALAQLPLLIGHLRTDEEFNKYL
                     QKKKEFSPFTSLFNQGGLPAMTVPVMFHHHLPVSVQMGSAKGNDLLLLELAHELQSVL
                     PKNITPASIP"
     gene            3766..5223
                     /locus_tag="I9760_002058"
     CDS             3766..5223
                     /locus_tag="I9760_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT5043821.1"
                     /translation="MKTLPSKVIFIGAGPSGLFAARQLKEIAKLQNKTVECIFLEREP
                     VVGGKCHTYSDAAHPELKTEWGAALVAPNYGVVLDALAEHGIEFEKVMPTASETIEIK
                     QLFEKASSLEKISMEMKLLRELWAFNSDYDVYKKAKQNKKALPDQLLNPFSEYCKLNN
                     MSYLPVLLKPFVPGFGYGDLNFCPAYCVLEYMGKVTMPDILLADQLIGRPSLLAIHGG
                     FQLLMERIAEQFDVRTSASVSRIKREKNKVTVTYVHNGIEQTETADTLVLATSPKNWK
                     SLGLDLTEVENNCIESLDYYRYPVAVYKIKGLPPQQYFFTKALEERGFGHLALITTRD
                     NRSNPEDGRLCTVYVNLSPNNNEFKFDHEALRKELESIPDVTEVTVVKDKIWEDYMST
                     LPWDLRLQLDKEQQRTNTMYLGSYALGSFEDVACVANKATDAMTEWYAPTVRYEEDFS
                     WKNMNRAWQFFTSPTKPPLASLGEPRVSDEHSNRP"
     gene            5463..5678
                     /locus_tag="I9760_002059"
     CDS             5463..5678
                     /locus_tag="I9760_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAT5043822.1"
                     /translation="MSVKETGHVKWFNDDKGYGFISRDNGQDDVFVHFRSIVSNAGRK
                     TLLEGQRVEFLVTKGAKGLQAEEVTAI"
     gene            complement(5778..7082)
                     /locus_tag="I9760_002060"
     CDS             complement(5778..7082)
                     /locus_tag="I9760_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAT5043823.1"
                     /translation="MTQQDPVVIKSKGDQALEKGDLPLALTLYDEALAINPQFSGAYY
                     QKGTVLLRMGKTIQAAAMYWQAYLFSEFKTDIGLMTAKTLAHANYSLSACKLFESFKI
                     EEMDGESLAYYLYALRQQGRVQEAYSLFPYVNQFNIPKTSWARAIILLDLNKVEEARF
                     LLEPLEKTDKDGHITILLHAVYLALNDKKAVKSLLDRAIQRIPNNDYFCCQRIAIDIL
                     NGLNKTPIETYRAFKRFDLIDAADYLHKHADKHLRHSGTTYQTFDLLAPQVLSEGLIL
                     EFGVRFGYSISHIAKLFPKRKIFGFDSFQGLPEDWHHEKAGSYSTYGKIPSVASNVTL
                     IAGWFNETLPDFKKNNREPIAFMNIDCDLYSSTKLVFNELNPQIAPGTIIVFDEYIGN
                     INWRQDEFKAFQEWVKENKVEYRYLTASFYTKQVSVQILKRK"
     gene            7245..8225
                     /locus_tag="I9760_002061"
     CDS             7245..8225
                     /locus_tag="I9760_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAT5043824.1"
                     /translation="MIKPITLLLLFCSFTVNASILVNGTFEATKSCPAYLSKNRKSNP
                     DNLMVEPNQKYQLKEINKTKPDWLRIQFSDHQPNLRWVSVDCGVIEYTERPKASCDLS
                     PGMADSYVLALSSQPGFCETYGYEIGKPECLKLTKTVYQANHLILHGLWPNQEACGHN
                     YGYCGISPRTNHCDYSPLDLSSDVAQNLKKLMPSYNYGSCLERHEWNKHGSCQDLSTD
                     GYFSLAMRLTTEVNNTEFGQYLTAHRGDTVKLTTLRELIKRSFGENNTGKVTLSCKNG
                     ILVDIYVQLPALIPFKESLESLVDKASHSQHHDYCNSNVTISDFSNEKWL"
     gene            8363..9325
                     /locus_tag="I9760_002062"
     CDS             8363..9325
                     /locus_tag="I9760_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043825.1"
                     /translation="MLKRCYLLILLMFVLASCAHHKPQTPPAEVKKQGTSSTRQFRQV
                     SSFNQIVVQGRLNVNLHTGYNKPEVMLRGDPRDLVQVRTIVKQNTLYVSLGQGYPDYG
                     AVTVDIKTKFLNRFRYEGAGVVTGNNLRTSYLDLYLANEGTTRLAGNIGLQKLEAVGN
                     GVTQINGVSSRNLQIVLKGDPKVLISGFVNLRQLDMYGKGTLSLYWIKSDTLTIRAKK
                     AAKIQLAGIVNRLDVELWDFAQFKGKYLRAQRSFVKTHDKSVAEISAVNHQSSLATDA
                     SDIYYYNLSKTRADFMAFNGSVLDMREWGQSDLKDFDRYNKQFP"
     gene            complement(9393..9626)
                     /locus_tag="I9760_002063"
     CDS             complement(9393..9626)
                     /locus_tag="I9760_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043826.1"
                     /translation="MFFYNASLKNDELNASKIAVLVTGLASLLLIGNYSDEESYEDVS
                     PSFLSRMMEDTPNYPFTSDEEGFEVNIGIPFQL"
     gene            complement(9773..10558)
                     /locus_tag="I9760_002064"
     CDS             complement(9773..10558)
                     /locus_tag="I9760_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAT5043827.1"
                     /translation="MKINDFRYMRRGKHLSELQSEEANLLAPVNQRGKGTERALLVLH
                     GFSSSPAVYRYLIPNLKNYDAILCPVLAGHAESIEAFSKSKAADWLQSAKSACELLIN
                     QYQKVDVLGLSLGGLLACQLSQSYAINHLFLLAPALKLHMRINAMLKLAQILKCLGFR
                     QLRNAAGNIITDEHAEIAYRKLPISTIIEMLTLAQGYEWLPPTCPADLFLGIHDQVVA
                     SQQVEQLFCNLPNVKIHWLKNSAHVLPLDNDLNEIIDCINKSK"
BASE COUNT         3375 a         1912 c         2272 g         3316 t
ORIGIN      
        1 gatcagcaaa atcaggacaa gcaaaataag gcacaacaga ataaagttca aaataaaaaa
       61 aatgacaagc aagagggaga aattcagtcc gaagcgaatc aggagaaaca acttgctaaa
      121 gaacaatggt tgcgattaat acctgatgat ccgggtggat taatgagaga aaaattttta
      181 cgtgatcatt tacgtagaga gcgtgggtgg tatcaatgaa gaaattaatg ttatggggtt
      241 tattgagttt attttcatca ttatcttttg cagaaatcaa agttgaactt gagccttcgc
      301 aagtcacaat gggagaaacc tttaaattat ccattactca agaagatcaa caggggggcg
      361 gagttcccga tctgactgaa ttgcaaaagg attttgtgat tttgggtaca gaaaggcatg
      421 taaactattc cattattaat gggcaggcgc aatcacaaag tcaatggatt gtactcttga
      481 gagcgctaaa gccaggtatt tttactattc ctccaataaa agtaggtctt gaacaaagtc
      541 tgccaatcac tatcaatgtc cagggtagtg ccaatacaca agctttgcca gatgaggcta
      601 tacaaaagca ggacgttttc ctgacagctg aagtgaatga gaaaaacccc tatgttaacc
      661 aacaaattat ttatacggtc aaattataca atagcaaacg attgttagat gccgattatc
      721 aaggtccaaa agttgatgat gccttgctga ttccgttagg ggatgcgaaa cgttaccatg
      781 ctgttcataa caacattaat tatgttgttg aagagcaaac ttatgcgata tatccgcaaa
      841 aaagtggcac tttgaaaatc atatcaccga tttttaatgc tcttgtatat gactataacc
      901 cccaacgcac caaagcgacg gataaagcga ttaacttaac tatcaagcca atccctaaaa
      961 agtatcaagg caaattatgg ttgccagcaa aacaggttaa gttatccgag caatatgaaa
     1021 acagtaatca gactctcagt cagggaagta cgttaacacg tacagtgaca ttggaaggaa
     1081 taggcattcc agctcagtta ttgccaacac ttacctttca agaaacagat ttctttagcg
     1141 tttatccaga gaaaggggcg gaacgcaatc aggttaagca aggtgagcta attggcagtg
     1201 tcgaaatcaa ggtcacttat ttgttcaaca aagcaggcaa aacgacaata cccgaattgc
     1261 gtcttacctg gtttaatact gaaacaggta aagaagaaat cgctgtttta cctccaagga
     1321 gcatagatat catcccttct actgcggtca aggcaaatac tgcaaacact caagtcacag
     1381 gtaaagaaat gtctgccagc agtcaattag cgcctgattc tgcatcagtt gccaatgaat
     1441 caggaggttg ggctgtgaag ttggcaatat tttttggttt ggcctggtta atcactttaa
     1501 gcttatggat atggcaaaag cgaccgagag agtcatccag taaaaaggag tataaaaaag
     1561 ctttggatgc tttaaataaa gcttgtagtc aatgtaatcc caaaaaagct cgtgatgctg
     1621 ttttaaaatg ggcctctttg caatggcctg atgctccaat gcttaattta actgacttgg
     1681 cgcaattggt aagagacgcg catttaagga aacaattgca tctgctttcg caagtcttgt
     1741 accgaagtca ggaaaaaaca ttatggcgcg gggatgaatt attgcgggct gtgcaagcgg
     1801 taaaacgtaa cgaatctgtg aaaaagcgaa aaaacagtaa tttacctcct attaatccat
     1861 tttagaaatt aaatctgttt aagcggtggt ttttgccagt tattccttta ctgtcaatat
     1921 ggacaactta tttatcctct tttttctgta atcacgggac tactgccaga ttgtatgccg
     1981 tcataatttg gctaagctgg ctcaggcgac tatgataaca acagctataa tgttatagat
     2041 aaccttgtaa agagtaatgt tctctcttta taaagctctc tggctattgt aggaattgag
     2101 tatgcatgtt aatgagtata tccactatga tgtgcaggag cttgctgaac tgatcaaaaa
     2161 ccatcaggtt ggtgctgagg aagttttgcg gtgtgcgcaa gaacgcctat atcaagtgaa
     2221 tccttcatta aatgccgtag ttacagattg ttttgaatat gcgcatcaat gccttgctaa
     2281 aatgaaaggt aacgagccct attatggtgt tccgttatta gtcaaggatc tgggccatgc
     2341 catagagggc attcgactaa cggaagggtc aaggtttttt tcaaataatc tggctgagac
     2401 aaacagtgat ttgattaata aattgattgc tcttggtttt gtaccatttg caaaaacaaa
     2461 tacttcagaa ttaggccttt cctgcgttac tgactctaat ttatttgggc cttgcagaaa
     2521 tccttttgat actatgagaa cggcaggcgg atcttctggt ggttctgctg ctgccataat
     2581 ggcagggata gcgcctgtgg ctacggcaag cgatggaggc gggtcgattc gtattccagc
     2641 ggcttgttgt ggtttgtttg gattcaaacc gacaccaggt ctggttccct cagggccatt
     2701 ggttgatgaa gcatggtccg ggatggctac cagttttata ctaactcgtt ccatgagtga
     2761 tgctattgca ttgtttaaaa atgtaataaa ccccatgcga cttaacagac tatcgagaaa
     2821 gaaaaaaaaa ttacttatta ccgagttgac aggcgctttt gcagaggtac caattgcaag
     2881 ggaatgccag gaggcggtag agaaaataaa agaaatcctg aaaaattcag gtcacagatt
     2941 aaatgaagtg cacttgccat tggatctgca agttatcagt tcatgcgccc atactttaat
     3001 tgcagcgagc acgtatgcga aaataaaaac tcaagaacaa atcagtggca gaaaaccaat
     3061 gattcatgaa ttggaaccca taacatggga attttattac agaggaaaaa tggtctccgc
     3121 ttatgaattc attattgcca aaaacaaact atatcaatca ctaagacctc tgcatcaatt
     3181 attggaaaaa acagacattg tattaactcc tgcattggct caattacccc tgttaatcgg
     3241 tcatttgcgc actgatgagg aattcaataa gtatctacaa aaaaagaaag agttttcacc
     3301 ttttacttcc ctgtttaatc aaggtggctt acccgctatg actgtaccag ttatgttcca
     3361 ccatcatttg cctgtctctg tgcaaatggg ttcagcaaaa ggcaatgatt tgctcttatt
     3421 ggagttggct catgaattac agtctgtgct gccaaaaaat atcacgcctg catcaattcc
     3481 ttgatgctga ttttttgtaa gttttcattt atcgaacctt gtgttaagtt aactgtagct
     3541 tttcataaaa ttaataataa gtgattgttt ttttatagct atagtcactt gaattttaaa
     3601 taatcgattc tctacgtact gctgcttaac gcggttttta aaaatcaagt cactatatat
     3661 tttttcaact tcagggcatt ttgcgtaggc agtaaatagt tccagtttga gtagttgtat
     3721 ttaataaagg taagttgtaa atggatttca ccaaagagga tttcaatgaa gaccttacca
     3781 tccaaagtca tttttatagg ggcaggcccg agcggcttat tcgctgcacg ccaattaaag
     3841 gaaatagcaa aattacaaaa taagacagta gaatgtatat tccttgaacg tgaacccgta
     3901 gtgggcggca aatgccacac ctactcggat gcagctcatc ctgagttaaa aacggaatgg
     3961 ggagccgcat tggttgctcc caactatggt gttgttcttg atgcattagc tgagcacggc
     4021 attgaatttg aaaaggtaat gccaacagct tctgaaacga tagaaatcaa gcaattattt
     4081 gaaaaagcca gttcgttaga aaaaataagc atggaaatga agttgcttag agaattatgg
     4141 gcttttaata gtgattatga tgtttataaa aaggcaaaac aaaataaaaa agcacttcca
     4201 gatcaattgt taaacccctt ttctgaatat tgtaagctga acaacatgtc ttatttacct
     4261 gtccttttaa agccatttgt gcctggattt ggttacgggg atttaaactt ttgtccggct
     4321 tattgtgttc ttgagtatat gggcaaggtc accatgccgg acatcctctt agccgatcaa
     4381 ttaattggca ggccctcact attagcaata catggcggtt ttcagctttt aatggagaga
     4441 atcgcggaac aatttgatgt gcgtaccagt gcttctgttt ccaggattaa gagagaaaaa
     4501 aataaggtga ctgtgactta tgtgcataat ggtatcgagc aaacagaaac agccgatact
     4561 ttggttttgg cgacttcacc aaaaaactgg aaatctctgg gtttagactt aactgaagtt
     4621 gaaaacaatt gtatagagag tctggattat tatcgctatc ctgttgctgt ttataaaatc
     4681 aaaggattgc cgccgcaaca gtattttttc acaaaagcac ttgaggaaag gggctttggg
     4741 catttggcgc ttattacaac cagggataac aggagtaatc cggaagatgg acggttatgc
     4801 acggtttatg tgaatttatc tcccaataat aatgaattta aatttgatca tgaagcgcta
     4861 agaaaagaat tagagtctat cccggatgta actgaagtta cggttgtaaa ggataaaatc
     4921 tgggaagatt atatgtcgac tcttccctgg gatttgcgat tgcaattgga taaagagcaa
     4981 cagcgcacga atactatgta tttgggctct tatgctctgg gaagttttga agacgtagcc
     5041 tgtgtggcaa ataaggcaac cgatgccatg acggaatggt atgctcccac tgtgaggtat
     5101 gaagaggatt tcagctggaa aaatatgaat agggcctggc agttttttac atcgccaacg
     5161 aaaccgccac tcgcttcgtt gggtgagccc agggtgtcgg atgagcacag taacaggcca
     5221 tagacggtaa gataaaagtg gtacatttat cgaagatatt gtacataatg ctcgctattt
     5281 gtagaatagt atattataat ataattactt gttctacgag cattattggt taaaatcttg
     5341 tttttcgaca aactccttcc ttatatttcc cttggtcgtg aatttctcat cgatgattac
     5401 attgtatttt ttctcttatc gctcattaca agtgtttgtt taattttatt tggaactttg
     5461 aaatgtctgt gaaagaaact ggccacgtta agtggtttaa tgatgataaa ggttatggct
     5521 tcattagtcg tgataatggt caagacgatg tatttgtaca cttccgctct atcgttagca
     5581 acgctgggcg caaaactttg ctagagggac aacgagtgga gttccttgtt actaagggag
     5641 cgaaaggttt gcaagcagaa gaagtaactg ctatataatc ccgtattcac tgaattacct
     5701 tcctctttgt ctaaaaaaac gaagaggaag gtggtttttt aatgccaata aattgcatta
     5761 attttttcaa ttatttttca ctttctctta agaatttgta ccgaaacctg tttggtataa
     5821 aagctggcag taagataacg atattccact ttattttctt ttacccattc ttggaaagct
     5881 ttaaattcat cctggcgcca attgatatta ccaatgtact catcgaatac tatgatagta
     5941 cctggtgcaa tctgggggtt taattcgttg aacaccaatt ttgtggaaga atagagatca
     6001 caatcgatat tcataaaagc tatgggttcc ctgttatttt ttttaaaatc tggcaaagtt
     6061 tcattaaacc atccagcgat taaagtcaca ttacttgcta cggagggaat tttaccatag
     6121 gtggaataag agccagcctt ttcgtggtgc caatcttcag gtaacccttg aaaactgtca
     6181 aaaccgaaaa ttttgcgttt gggaaagagt ttggcaatat gggaaatgga atagccaaat
     6241 cgcactccaa attccagtat taagccttca gaaagaacct gtggtgctaa taaatcaaat
     6301 gtttgataag ttgttccact atgtcgcaaa tgtttgtcag catgtttatg aagataatca
     6361 gctgcatcaa tgagatcaaa gcgtttgaat gcgcgatacg tttctatcgg tgtcttgttt
     6421 aaaccattga gtatgtctat tgcaattcgc tggcagcaaa aataatcatt attgggtatg
     6481 cgttgaattg ctctgtctaa aagagatttt actgcttttt tatcatttaa agccagatat
     6541 accgcatgca gtaaaatggt gatgtgtcca tctttatccg ttttttcaag tggttcaagt
     6601 aagaagcgcg cttcctctac tttattaagg tcaagcaaaa taatggctct tgcccaactt
     6661 gttttgggga tattgaattg gtttacataa gggaacaagg aatacgcttc ttgtaccctt
     6721 ccttgttgcc ttagagcata gaggtaatag gcgaggcttt caccgtccat ttcttcaatt
     6781 ttaaatgatt caaaaagctt gcaggctgac aaagaataat tagcatgcgc cagagttttt
     6841 gctgtcatta atcctatgtc agttttgaat tcgctgaata aataagcttg ccagtacatg
     6901 gctgctgctt gtattgtttt acccatgcga agtaaaacgg ttcctttttg ataataggct
     6961 ccggaaaatt gtgggtttat cgctaatgct tcatcataca gtgttaaggc aagaggcaaa
     7021 tcccccttct ctaaagcctg gtctcccttg gatttaatca ctacaggatc ttgttgggtc
     7081 atttattttc tccagtacct acctctatat catagtattc ttttattcgg aatgtagttg
     7141 tattgaaaaa atccttatta atcaatttca tgaaaaaata attgctggca ttcatatccg
     7201 actagggtat aatgggctta ccaaagacaa gccaggatgt cgtaatgata aaaccaatca
     7261 ccctgctttt gcttttttgt tctttcactg ttaatgcctc aattctcgtc aatggtactt
     7321 tcgaagcaac aaaatcatgc cctgcttacc tttccaagaa tcgtaaatca aatcctgata
     7381 atttgatggt tgagcccaac cagaaatatc aattaaaaga aattaataaa accaaaccgg
     7441 attggttgcg tatacaattt tcagatcatc aacctaatct gcgctgggtg agtgttgatt
     7501 gcggggtcat tgaatacact gagcgtccca aagcgagctg tgatctttct cccgggatgg
     7561 ctgattctta tgtgttggct ttaagttcac agccaggttt ttgtgaaact tatggatatg
     7621 aaataggcaa accagagtgt ttgaaattaa caaagacagt ttatcaggcc aatcatctga
     7681 tcctgcatgg attatggcct aatcaagaag catgcggcca taattatggc tattgtggaa
     7741 taagccctcg aacaaaccat tgtgattatt ctccattgga tttatcttct gatgttgctc
     7801 aaaatttaaa gaaacttatg ccgagttaca attatggcag ttgtctggag cgccatgaat
     7861 ggaacaaaca tgggagctgt caggatttaa gcacggatgg ttattttagt ttggctatgc
     7921 gcttgactac agaagttaat aatacagagt tcggtcaata tcttaccgct catcgtggtg
     7981 atactgtaaa attgactact ttgcgtgaat tgattaaaag atctttcggg gaaaacaata
     8041 caggtaaagt gactttaagt tgcaagaatg ggatcttggt ggatatttat gtccaattac
     8101 ctgctctcat accttttaaa gagtctctag agtctttggt tgataaagcc agccactcac
     8161 aacaccatga ttattgtaat agcaatgtca ccatttctga ttttagtaat gaaaaatggt
     8221 tataggtatt caagtggagg agatggctcg ttcacaaaac ttttttaaaa taatatggca
     8281 ttgcttcctt ataggagact gccttgcaca ttgtcaaaac ttcctctaat gtagagagat
     8341 tattgatata ggaatacaga ttatgctaaa gcggtgctac ttattgattt tacttatgtt
     8401 tgtattggca agctgcgccc atcataaacc gcaaacacct ccagcggaag ttaaaaaaca
     8461 aggtaccagc agtacacggc agtttcgcca agtgtcatcg ttcaatcaaa tcgtagttca
     8521 gggacgactg aatgttaatt tgcataccgg gtataataag ccagaggtca tgttacgcgg
     8581 tgatcccaga gatttggttc aagtaagaac gatagtaaaa caaaatacac tctatgtgtc
     8641 tttgggccag ggatacccgg attatggtgc tgtgactgtg gatattaaaa ccaaattcct
     8701 caaccggttt cgctatgagg gcgctggggt tgtgacaggg aataatttac ggaccagcta
     8761 cttggacttg tatttagcga acgaaggtac caccagactg gcaggaaaca ttgggttgca
     8821 aaaactcgag gcagtcggta atggcgtgac acaaatcaat ggtgtatcca gcaggaattt
     8881 gcaaatcgta ttgaaaggag atcccaaagt actgatatct ggttttgtca atctaagaca
     8941 gcttgatatg tatggaaaag gcacgttaag tttgtattgg ataaaaagcg atactttaac
     9001 cattagagca aaaaaggcgg ccaaaattca attggctgga attgtgaatc gactggatgt
     9061 tgaattgtgg gattttgctc aatttaaggg gaaatattta cgagctcaac gtagttttgt
     9121 gaaaacccat gataaatccg tagcagaaat ttcagctgtg aatcatcaaa gcagccttgc
     9181 tactgatgcc agtgatattt actattataa cttatcaaaa accagggctg atttcatggc
     9241 atttaatggc tcagtgctgg atatgcgtga atggggccaa tctgatttaa aagattttga
     9301 tagatacaat aagcagtttc cctagcattg attcaggata aaagagctaa attcataagg
     9361 gtaaaccctt ggtgaattta gcttgctata aattagagtt gaaacggaat ccctatgttg
     9421 acttcaaatc cttcctcatc ggatgtaaat ggataattag gagtatcttc catcatccga
     9481 ctaaggaaag agggagagac atcctcgtag gactcttcat cactataatt tccaatgagt
     9541 aataatgagg ctaatcctgt aacgagaaca gcaatttttg atgcatttaa ttcatcattt
     9601 tttaatgagg cattataaaa aaacatgttt aatttccaaa gaacaaaatg agccgttaaa
     9661 atacaatgat tttttttctt attcaatatc taaatcgcta taacgcttgc aagtctaaag
     9721 aagaggttgt ttgattggtt acaaatcaat catggcaatt ttttatttcc attcatttgc
     9781 ttttattaat acaatcaata atctcattca gatcgttgtc tagcggcagt acatgcgcgg
     9841 aattttttag ccaatgtatt ttgacattag gtaaattaca aaataattgt tctacttgtt
     9901 gagaagcaac cacttgatca tgaattccaa gaaaaagatc ggccgggcaa gttgggggga
     9961 gccattcata tccctgagcc aaggttagca tttcaatgat tgtggaaatg ggaagttttc
    10021 tataagcgat ttcggcatgc tcatcggtga taatattccc tgcagcgttg cgaagttgtc
    10081 gaaatccgag gcacttaaga atctgggcta atttcagcat ggcgttgatg cgcatgtgta
    10141 attttaatgc gggggctaat agaaacaggt gattgatagc ataggattga cttaattgac
    10201 aggctaataa tcctcccagg gataatccta aaacatcaac tttttgatac tgatttatta
    10261 agagttcaca ggcgcttttt gctgattgaa gccaatcagc tgctttggat tttgaaaagg
    10321 cttctatact ttcggcgtga ccagccaata caggacaaag gatggcatca taatttttta
    10381 aattaggaat cagatatcgg taaactgccg gtgaagaaga gaaaccatgc aataccagta
    10441 aagctcgttc tgtgccttta ccacgctgat tgactggagc aaggagattt gcttcctccg
    10501 actgcagttc gctgagatgt ttgccgcggc gcatataccg aaaatcattg attttcatag
    10561 aatattcatt tttcattgtc atgccatgta tggttgctat agtaattggt ttaatttggg
    10621 gtgaataggc tttccaaaag aaagtgtatt accaaatata aaaatgtaaa agttataatt
    10681 gtatcagttt attttagtat aagtactgtg ctatttttct ttgtcatata agaactttaa
    10741 agtgtaaatc tttttgtctt attcggggca atagtctttt gcaaaatata taaagacact
    10801 gttttaatgg aaattatcta aagtttaatt aaatagaaat cgagattaat aattacagtt
    10861 agtttataga gtcag
//
LOCUS       DACTJE010000106        10746 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.106, whole genome shotgun sequence.
ACCESSION   DACTJE010000106 DACTJE010000000
VERSION     DACTJE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.106"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(215..982)
                     /locus_tag="I9760_002065"
     CDS             complement(215..982)
                     /locus_tag="I9760_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine synthase"
                     /protein_id="HAT5043828.1"
                     /translation="MNPIKPPFTLNQYFAAWFVHVFTASAACIGVFSLYKIYQHDYVF
                     ALWLMAITVFIDAVDGSLARLVHVKSVLPKIDGALLDNIVDYLNYVITPCFFLLVKPG
                     MLPADYVVPITAAITITSAYQFCQDDAKTPDHFFKGFPCYWNITVFYMYIFNTSMIVN
                     TVLLSLFCVLIFIPVKYVYPSRLDYLTESRVLKILMHCCSALYGISSFCLLVNYPETN
                     KLWVSLSLGYVGMYLFLSFYRTYYPMFKAKITANNKD"
     gene            1313..2701
                     /locus_tag="I9760_002066"
     CDS             1313..2701
                     /locus_tag="I9760_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAT5043829.1"
                     /translation="MNIQTVNPTTEEVLNIYTCLDFDSIDKKIACGYEAYLNWKNSKF
                     DIRRSLMLNLAQILKDKKQELAHLMALEMGKPIAAGRAEIEKCAWVCEHYAENAEQYL
                     MPRTVQTEMKTAKVCYRPLGIVFAIMPWNFPFWQVFRFSAPTIMAGNGAILKHAPISS
                     GTGNKIEELFRDAGFPSSLFQHLIVDNDGASHIIENQFVTAVTLTGSERAGQSVAANA
                     GKNLKKSVLELGGSDPYLILEDADLDLAAQCIVASRLNNTGQVCIAAKRVIALQSIEK
                     ELISKIKELISKYKMGDPLDPNINLGPMARKDLRENLHSQVEKSVKQGAKLLLGGIIP
                     EGKGYFYPPTLLTDVKPGMTAFDEELFGPVIVIITAKNESEAILLANMSQYGLGAAVF
                     TKDLNKGEHIATYEIEAGTCFVNAFVASDPRLPFGGIKRSGYGRELSREGILEFVNTK
                     TIAISDPKHGKK"
     gene            2688..3176
                     /locus_tag="I9760_002067"
     CDS             2688..3176
                     /locus_tag="I9760_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAT5043830.1"
                     /translation="MVKNSKFIFISIKKQEMSCFEKDHLIANYAISSGKNGVGEKINS
                     GCTPRGWHKIYSILGIDLPINSVMVAREWTGEIYSESLATQFPERDWILTRVLQLDGL
                     EPGRNKGGDVDTLHRYIYIHGTPDTTQLGIPGSHGCIRLNNDAIIELAAWATKNTLVY
                     IQ"
     gene            3228..8105
                     /locus_tag="I9760_002068"
     CDS             3228..8105
                     /locus_tag="I9760_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HAT5043831.1"
                     /translation="MSYKFEEGKDAIVKSVVEKIKQTMTGEQTEFCAEFAKQFYGTVA
                     LEDLLEWEIDDLYGAAVNFWSLICERAPHETKIRIYNPDYERHGWQTTHTVVEVICED
                     MPFIVDSLRIVINRMGLTSHLTIHMGGIRVKRDSHNRVTEILPRNGGAARDDVLHEAP
                     IFIEIDRQTDPATLTELHKNFERALEDNRAVFEDWDKMRTKVREIIKELDTVPKTIDI
                     SEIEETKAFLNWMEDHHFTFLGLRDYDLVKKGKETILQPIPETGLGVLRQSLSKSNAR
                     SITAMGPEAQGLISSPRILVMSKTNTLASVHRDAYTDYIGVKRFNKKGEVIGERRIIG
                     LYTSAAYHTNPKHIPFLRHKVALIMKNSNLNPYSHAGKVLLNILETLPRDDLIQGTED
                     ELLEIAMGIFYMQDRKRIRLFARADVYKRFISCLVYVPKDRFNTELRYAMQKILADSF
                     NAEEITFSTQFSESVLARIHFIVRVNPKNLPDFDLKEIEQKLIEVGRSWIDDLQHHLH
                     EVYGEEQANSLYSRYKNAFPISYSDTFSPRTAVYDIKHIEMLSPENSLGINFYKPLDE
                     SEKSFRLKVYQHDTTIPLSDVLPILEKLGLRAISERPYVLKFEDGKVAWINDFAMQYN
                     KESEFNIDEIKELFQNAFARVWFGDAENDGFNLLVLAAGLNWREVAVLRTYAKYFRQI
                     GFTFSQDYMETALNNNVSIAKKLVRLFEIRCNPHDSKKREDRYVALVAEILSDLDNVA
                     NLDEDRIIRQYVHAIGATLRTNFYQVDAQGNPKNYISIKLSSKLIPGVPKPYPMFEIF
                     VYSPRFEGVHLRCGKVARGGLRWSDRREDFRTEILGLMKAQQVKNSVIVPSGAKGGFV
                     PKQIPANATREEIMEEGISCYKLFIRGLLDITDNYKEGLIVKPQNVVFYDEDDPYLVV
                     AADKGTATFSDIANSISQEYDFWLGDAFASGGSVGYDHKKMGITAKGAWESVKRHFYE
                     LNRDIQNNDFTVVGIGDMAGDVFGNGMLLSRHIKLVGAFNHIHIFIDPNPEAEASFKE
                     RERLFNLPRSNWTDYDKKLISKGGGVFSRSAKSIPVSPEMQKVFGIKQTSIEPNDLIK
                     AILKADVDLLWSAGIGTYVKSSTESNTSVGDRTNDATRVNANQLRCKVIGEGGNLGLT
                     QLARVEYSLHGGKVYTDFIDNSGGVNCSDKEVNIKILLNNVVSAGDLTPKQRNELLSN
                     MTDEVAKLVLRDNFLQTRAISLTASQALRAIELHARYINELEKTGKIDRTLEFLPDEK
                     VLMEHKLMGKGLTQPGIAVLMCYSKTILKEQVLASEVPEEDYMNQILIGSFPKPLQER
                     FSKQMQDHPLRREIIATRLSNIIVNEMGFTYVYRLQDETGASVAAIVRAYMIARSVLN
                     LEAIWKQIEELGTKVSAQAQVDMMMLYVRLSRRVTRWFLRTHRRSMSITQAIELYAKG
                     VDELKKSMPAVFGETGRIQYEEHYQERIKEGIPPQLAHELTVTRGLFAATDIIEIAYE
                     ENIKISKVAEIYFGIGEFLDIAWLRTQIIVHTTENHWESLSREALRDDLDWQQRQLTA
                     AIMGFEPNNKDLQERLTRWGETHVSLIDRWNYILTALKSSTALNYTMFLVATRELLDL
                     TQTTLQSNLKMEESF"
     gene            8126..8674
                     /locus_tag="I9760_002069"
     CDS             8126..8674
                     /locus_tag="I9760_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAT5043832.1"
                     /translation="MEAKTASEYSTLSKLFHWIIALAVIIMLIAGFFLDEIPEQFQGV
                     AYMLHKSTGITILFLMILRFIWIHAKGKPALPASVKSWEKILSRSVQYGFYVLLIVMP
                     LSGWIMSVAADRIPNYFGLFKAPLPWIEPNKSLAKLMAESHEIIAWILIAFITLHVLG
                     ALKHHFIDKDNVLKRMLPGKSK"
     gene            complement(8705..9763)
                     /locus_tag="I9760_002070"
     CDS             complement(8705..9763)
                     /locus_tag="I9760_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HAT5043833.1"
                     /translation="MISDFERPNDTLLCIINNITDLDMKTMLEHLVNNRSLITFRPIE
                     SELLLNKQKNYLFDLSYLGVIDLFGEKSIDFLQGQLTCDLRLVSDVSMVQGAQCNLKG
                     RILSLLDIINWQGVKLVLPQDLIEVTQNSLNKVALLSRVKITSNNNYKILGFYLQHDN
                     DQIPQLLPLPSELYALSCTAHGCVYHLGKGFYIYLIHSDYYDSLCKPFIENNQLLGSL
                     TWHTLRLFNNQIDIYPNSRGLFLPHRIGLHQTTYVSFDKGCYKGQEIIARTHYRATLK
                     HELKKFVIQSDNQLYSGQKLFKSDENTEVGELIDYSMLGENRYLVAASILKENFSSVC
                     LEGNKFPIQLEGKIELEL"
     gene            9820..10059
                     /locus_tag="I9760_002071"
     CDS             9820..10059
                     /locus_tag="I9760_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HAT5043834.1"
                     /translation="MDNREKSRLLWKCRRGMLELDLVLQKFIANEIDRLTENQLKAFD
                     NLLTHNDPNLYAWLMGHEEPEKELLEIVSFIRNCD"
     gene            10001..10450
                     /locus_tag="I9760_002072"
     CDS             10001..10450
                     /locus_tag="I9760_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1578 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043835.1"
                     /translation="MRNPKKSCLKLYHSSETVIKPGKSKVYLRLITILYLCTIGLVLY
                     SSLPVLIEGVLIFLIVIQFKNDVTYQSACQKVKEIKCLKDLTWSLETIDGTTHHYDKL
                     KILIHNMLFQVIQLSSDKKNQMLILFNDQIPTDQLRLLHLNSNKNSI"
     gene            10544..>10746
                     /locus_tag="I9760_002073"
     CDS             10544..>10746
                     /locus_tag="I9760_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HAT5043836.1"
                     /translation="MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTH
                     DWTNVNDLTQEVLIKVFRHLPYF"
BASE COUNT         3650 a         1731 c         2112 g         3253 t
ORIGIN      
        1 atatctttgt tatcaggtat tcataagtta ttggcataat ttaacttcta tagaggcgta
       61 gattttatat caattgcttg cattacctct ctattttttc agtaaacaga tactatttaa
      121 agcaataaga acagaaaaat tcctaaaaga gtaagataaa acagtgccgc cagaaatgta
      181 atatctgcat ggaaaacata aattacattt aaactcaatc tttattattt gccgtaatct
      241 tagctttgaa catcggataa taagtcctgt agaaacttaa aaataaatac atgcccacat
      301 agcctaaaga taggcttacc cataatttat ttgtttcggg gtaattaact agcaggcaaa
      361 atgaactgat gccataaaga gcagaacaac aatgcattaa tattttcaag actctcgact
      421 cagtcaagta atctaacctt gatgggtaaa catattttac tggaataaaa atcagtacac
      481 aaaaaaggga caagaggaca gtgttcacaa tcatagaagt gttaaaaata tacatataga
      541 agacagtaat attccaatag caggggaacc ctttaaaaaa gtgatcagga gttttggcgt
      601 catcctggca gaattgatag gctgatgtaa ttgtaattgc ggctgtaatt ggtacgacat
      661 aatcagctgg cagcataccc ggtttaacta gcaagaaaaa acatggagta atgacataat
      721 tcaaatagtc aactatattg tctaataaag cgccatcaat tttaggcaat acgcttttta
      781 catgaaccaa tcgagcaagg ctaccatcta ctgcgtcaat aaatacagta attgccatta
      841 gccataaagc aaatacataa tcatgctggt aaattttata taagctaaag acaccaatac
      901 aggcagcgct ggcagtaaat acatggacaa accaggcggc aaaatattga ttaagggtaa
      961 agggaggctt tattggattc attacttatc ctagagttaa tcaatgtaat tagggcttca
     1021 gtcttttttg taaaagcaaa aaacaagtta cctttaaaaa tatccagcgc aatgattaaa
     1081 tctggattgt tttgttcatt aagacgatgt gtttttgctt tttactaaaa cagtacaagt
     1141 cctttttact taacaggttg atttttatta ctgttcttta agacaatgtc gtttaaaata
     1201 aaaatagtat caatattcaa aagcaaagtg caagttaggt taattattta aaataaaaaa
     1261 agctttgatt tggtctaaac tataggcaac gttaatcata tcgagtaatt aaatgaatat
     1321 tcaaactgtc aacccgacaa ctgaagaagt tctaaatatc tacacatgct tggattttga
     1381 ttcaatagat aaaaaaatag cgtgcggata tgaggcttat ttaaattgga aaaattctaa
     1441 attcgacata aggcgctctt taatgctcaa tcttgctcag atattaaaag ataaaaagca
     1501 agagttagcg catttaatgg ctttagaaat gggtaaacct attgcagcag gtcgtgctga
     1561 aattgagaag tgtgcttggg tatgtgaaca ttatgctgaa aatgcagaac aatacctaat
     1621 gccaagaact gttcaaactg aaatgaaaac agcaaaagtt tgttacagac cactaggaat
     1681 tgtatttgcc ataatgcctt ggaatttccc attttggcag gtttttagat tttcagcacc
     1741 aactatcatg gcgggtaatg gggctatttt aaaacatgct ccaatatcga gtggaacagg
     1801 gaataaaatc gaagaattat tcagagacgc tggatttcct tcttcattat ttcaacactt
     1861 aattgtagat aatgatgggg catctcatat cattgaaaat caatttgtta ccgcagttac
     1921 tttaactgga agtgaacgtg ccggtcaaag tgttgcagcg aatgcaggta aaaatttgaa
     1981 aaaatcggtt ctagaattag gcggttcaga tccctatttg attcttgaag atgcggattt
     2041 agatcttgct gctcaatgca ttgtggcttc acgattaaat aataccgggc aggtctgcat
     2101 tgcggccaag cgagttatag cacttcaatc gattgaaaaa gaattaattt ctaaaatcaa
     2161 agaactcatc agcaaataca aaatgggaga tccattagac cccaatatta atttgggacc
     2221 catggcaaga aaagatttac gagaaaattt gcattcacaa gtagaaaaat ctgttaaaca
     2281 aggtgctaaa ttattgcttg gtggtataat tccggagggt aaagggtact tctacccgcc
     2341 gacattatta actgatgtta aacctggaat gacagctttt gatgaggaac tatttgggcc
     2401 tgttattgtt attattactg ctaaaaatga gagtgaggca attttactgg ccaatatgag
     2461 ccagtatggt ttaggtgctg ctgtatttac aaaagatctt aataagggag agcatattgc
     2521 tacttatgaa attgaagcgg gaacctgttt tgttaatgct tttgtagcct ctgatccaag
     2581 acttcctttt gggggaataa agcgatcagg atatggtaga gagttatcaa gagaaggtat
     2641 attagagttt gtaaatacca aaacaattgc gattagtgac ccaaaacatg gtaaaaaata
     2701 gtaaattcat ttttatttca ataaaaaaac aggagatgag ttgttttgaa aaggatcatt
     2761 taattgcaaa ctatgctatt tcatccggga aaaatggcgt gggtgaaaaa attaatagtg
     2821 gatgcacccc gagaggttgg cataagatct attctatact tggcattgat ctgccaatta
     2881 atagtgtgat ggttgccaga gaatggactg gtgagattta ttcagaatca ttggcaactc
     2941 aatttccaga aagggattgg atattaaccc gagttttaca attggatgga ttagagcctg
     3001 gtcgaaacaa gggcggagat gtagatactt tacatcgtta catttatatt cacggcacac
     3061 cagatacaac tcaactaggt atacctggct cacatggatg cattcgcctg aataatgatg
     3121 caattattga gctggcagct tgggcaacaa agaatacttt agtatacata caatgattaa
     3181 taacaattaa tgcttcggag cataagtgaa cctgaagggg taaatgaatg tcctataaat
     3241 ttgaagaagg taaagacgca atagtaaaaa gcgttgttga aaaaattaag caaacaatga
     3301 ctggcgaaca aacagagttt tgcgctgaat tcgcgaagca attttatggg actgtcgccc
     3361 ttgaagatct tttagagtgg gagattgatg atctttatgg agcagcagtc aatttctggt
     3421 ctttgatttg tgaacgcgct ccacacgaaa caaaaatcag gatttataat cctgattatg
     3481 aacgtcatgg atggcaaact acacatactg ttgtagaagt tatctgcgaa gatatgcctt
     3541 ttattgtaga ttctctgagg atagtaatta atcgaatggg attaacatct catcttacta
     3601 tccatatggg aggaattagg gtgaaaagag acagccataa cagggttact gaaattcttc
     3661 caagaaatgg tggagctgct agagatgatg ttttacatga ggctcccatt tttatagaaa
     3721 ttgatcgaca aacagacccg gctaccttaa cggagctgca caaaaatttt gaacgcgctc
     3781 ttgaggacaa tcgagcggta tttgaagatt gggataaaat gcgtactaaa gtacgagaaa
     3841 ttattaagga gcttgatact gtaccaaaga caattgatat cagtgaaatt gaagagacga
     3901 aagccttttt aaattggatg gaagatcacc attttacttt tttaggatta cgagattatg
     3961 atttggttaa aaaaggaaaa gaaaccattt tacaacctat tcctgaaaca ggattgggcg
     4021 ttttgcgcca aagtctgagc aaatcaaatg caagaagtat tacggcaatg ggaccagagg
     4081 cacaaggatt aatttcatca ccccgtattc ttgtaatgtc caaaacaaat actttagcct
     4141 ctgttcatag agatgcttat accgattata ttggtgttaa gcgcttcaat aaaaaaggag
     4201 aggtgattgg cgaaaggcgc attataggac tgtatacttc tgcagcttat catacaaatc
     4261 ccaagcatat tccgtttttg cgacataaag tcgcattaat tatgaaaaat tctaatttaa
     4321 atccctatag ccatgcaggt aaagtattat tgaatatact tgaaactctt ccaagagatg
     4381 atttaattca aggcactgag gatgagttac tggaaatagc tatgggtatt ttctatatgc
     4441 aagatagaaa acgtatccgc ctgtttgcca gagccgatgt ttacaagcga tttatttcat
     4501 gcttggtata tgttcccaag gatcggttta atactgaatt aagatatgcc atgcaaaaaa
     4561 ttttggcaga cagttttaac gctgaagaga ttaccttttc tactcagttt tcggaatcgg
     4621 tattggcaag aattcatttt atagtaaggg ttaatcccaa aaatttaccc gattttgatt
     4681 taaaagaaat agagcaaaaa ttgattgaag tggggcggtc gtggattgat gatttgcaac
     4741 atcatttaca tgaagtctat ggcgaagaac aagccaattc actttactct cggtataaaa
     4801 atgcgtttcc aatatcttat tctgatacct tttcacctag aacggctgtc tatgatatta
     4861 agcatattga aatgctaagc cctgaaaatt cgctcggtat taatttctat aagccattgg
     4921 atgaatctga gaagagcttt aggttaaagg tttatcagca tgacaccact attcctttgt
     4981 ctgatgtatt acctatcctg gaaaaacttg gtttaagagc aattagtgaa cgtccttatg
     5041 ttttaaaatt tgaagatggt aaagtagcat ggattaacga ttttgccatg caatacaaca
     5101 aagaaagcga gtttaatatc gacgaaataa aagagttatt tcaaaatgca tttgccagag
     5161 tatggtttgg tgatgcagaa aatgatggct ttaatttatt ggtattagcg gccggactaa
     5221 attggaggga agttgctgta ttaagaacct atgcaaaata tttcaggcaa atagggttta
     5281 catttagcca ggattatatg gaaacagcac taaataataa tgtaagcatt gccaagaaat
     5341 tagttaggtt atttgaaatt cggtgcaatc cccatgactc caaaaagaga gaggatagat
     5401 acgttgcact tgtcgctgaa atcttatctg atcttgataa tgtagccaat ctggatgaag
     5461 acagaattat aagacaatac gtgcatgcca tcggtgccac tcttcgtacc aatttttatc
     5521 aagtagacgc tcaaggtaat cctaagaatt atatttctat aaaattgtcc agcaaactca
     5581 ttcctggtgt accaaaacca taccccatgt ttgaaatttt tgtttactcg ccacgatttg
     5641 aaggggtgca tttgcgctgt ggtaaagtgg ctcgcggagg tttacgctgg tcggacagac
     5701 gtgaagattt ccgaaccgaa attcttggct taatgaaggc tcaacaagta aagaactctg
     5761 taattgtccc cagtggcgcc aaaggtggtt ttgtacccaa acagatacca gctaatgcta
     5821 ctcgtgaaga aattatggag gagggaatca gctgttataa actctttata agaggtttgc
     5881 tggatataac agacaattac aaagaaggac tcattgtaaa accccaaaat gttgtttttt
     5941 atgatgaaga tgacccttat cttgtggtcg ctgcagacaa aggcacagcg acattttctg
     6001 atattgccaa ttcaatttcc caggaatatg atttttggct tggagatgct tttgcctcag
     6061 gtggctctgt tggttatgat cataaaaaaa tggggattac tgctaaaggg gcatgggaat
     6121 ctgttaaacg ccatttctat gaattgaaca gagatatcca aaacaatgat ttcactgtgg
     6181 ttggcatagg agatatggcc ggggatgtct ttggaaatgg catgttgttg tccaggcata
     6241 tcaagcttgt aggcgctttt aatcatattc atatatttat agaccctaat cctgaagctg
     6301 aagctagctt taaagagcgt gagcgattat tcaaccttcc cagatctaac tggacagact
     6361 atgataaaaa actcatttcc aaaggaggag gggttttttc tcgtagtgca aaatctatcc
     6421 cggttagccc tgaaatgcag aaagtatttg gaataaaaca aacttcgatt gaacctaatg
     6481 atctaattaa agcaatatta aaagccgatg tggatttact ttggagtgct ggaattggaa
     6541 cctatgtcaa atcgagtact gaaagtaata ccagcgtagg agatcgaaca aacgatgcaa
     6601 ctcgtgttaa tgccaatcag ttgagatgca aagttatagg cgaaggtggg aatttgggat
     6661 taacccagtt agcacgagtg gagtatagtt tacacggcgg caaagtctat acagatttca
     6721 tagataattc tggtggggtt aattgctcag acaaagaagt taatattaag attttattga
     6781 ataatgttgt atcagctggg gatctgactc caaaacaaag aaatgagctt ttaagtaata
     6841 tgaccgatga agtagccaaa ttagtgctca gagataattt cctacaaacc cgagccatta
     6901 gcttaactgc ttctcaagcc ttaagagcca ttgaattaca tgcaagatat attaacgaac
     6961 tagaaaaaac aggaaaaatt gacagaactt tagagttttt acctgatgaa aaagtcctaa
     7021 tggaacacaa actaatgggg aaaggattaa ctcaacctgg aattgctgtt ttaatgtgct
     7081 acagcaaaac catactaaaa gagcaagttc ttgcttctga agttcctgaa gaagattata
     7141 tgaatcaaat acttattggt tcatttccca aacctctgca agagcgtttt agtaaacaaa
     7201 tgcaagatca tcccttaaga agggagataa ttgctacccg tttaagtaac attattgtta
     7261 atgaaatggg cttcacgtat gtttacaggt tacaagatga aactggggcc tctgttgcgg
     7321 caattgtcag ggcctatatg attgccagaa gtgtcttgaa tctggaagca atatggaaac
     7381 aaattgagga acttggtaca aaagtcagcg cgcaagctca agttgatatg atgatgctgt
     7441 atgttcgact atccaggaga gtgactaggt ggtttttacg gacacataga aggtcgatga
     7501 gtataacgca ggcgatagag ttatatgcaa aaggggttga tgaactgaaa aaatccatgc
     7561 ctgccgtttt tggtgaaaca ggccggattc aatatgaaga gcattaccaa gagcggataa
     7621 aagaagggat accgcctcaa cttgcccatg aattaacagt gactcgaggc ttgtttgccg
     7681 caacagacat tatagaaatt gcctacgagg aaaacataaa aatatcaaaa gtagctgaaa
     7741 tatattttgg tattggcgaa tttcttgaca tagcttggct tagaacccaa attattgttc
     7801 acacaactga aaatcattgg gaatctttat ctcgagaggc attacgcgat gatttggatt
     7861 ggcaacaacg ccagttaaca gctgccataa tgggctttga gcctaataat aaggatttgc
     7921 aagaacgatt aacaagatgg ggagagacac acgtttcttt aattgacagg tggaactata
     7981 ttctgacagc tctgaaatca agtacagctt tgaactatac tatgttttta gttgctaccc
     8041 gagagttgct ggatttaacc caaacgacat tacaatccaa tttaaaaatg gaagagtcat
     8101 tttgacatga aaggagttaa atgaaatgga ggctaaaact gcttcagaat attcaacact
     8161 gagcaaatta tttcattgga tcattgcatt ggcagttata atcatgctaa tagcaggttt
     8221 ttttcttgat gaaatacctg agcaatttca gggggttgct tatatgcttc ataaatcaac
     8281 aggaattact atccttttct tgatgatttt gaggtttatt tggatccatg ctaaaggaaa
     8341 gcctgcttta cctgcgtctg ttaaatcttg ggagaaaata ttgtcaaggt ctgtgcaata
     8401 tggtttttat gtactcttga ttgttatgcc attgagtggt tggatcatgt cagttgcagc
     8461 agacagaata ccaaattact ttggcttgtt taaagcgcca cttccatgga ttgaacctaa
     8521 taaatctcta gcaaaattaa tggctgaatc ccatgaaatt attgcgtgga ttttaatcgc
     8581 cttcatcaca ctgcatgttt tgggtgctct taagcatcat tttattgata aagacaatgt
     8641 attgaagcgt atgctgcctg gaaaaagcaa atagccctct gtttttgaaa attgagcctg
     8701 aacttcaaag ttcaagctca atttttcctt ctaattggat tggaaattta ttgccttcca
     8761 aacagacaga tgaaaagttc tcttttaata tacttgcagc aaccaaatat cgattttcac
     8821 caagcatgga gtaatcaatt aactctccaa cctctgtgtt ttcatccgat ttaaataatt
     8881 tttgaccaga atataattga ttatctgact gaatcacaaa tttctttaat tcatgcttaa
     8941 gagtggctct atagtgagtt cttgcgatga tttcctgacc tttataacac cctttatcaa
     9001 aactgacgta cgtggtttga tgtaagccaa ttctatgtgg taaaaagagc cccctagaat
     9061 taggataaat atcaatttga ttgttaaata atctaagggt atgccaggtt aatgaaccaa
     9121 gtaattgatt attttcaata aaaggcttgc acaaactatc ataatagtct gaatggatta
     9181 aatagatata gaatcctttg cctaagtgat aaacacaacc atgagccgta caagataaag
     9241 catacaattc gctgggcaaa ggtagtagct gtggtatttg atcattatca tgctgtaaat
     9301 aaaagcctaa aattttatag ttgttatttg atgtgatttt aacacgagaa agcagtgcaa
     9361 ctttgtttaa tgaattctga gttacttcaa ttaaatcttg gggcagaact aattttacac
     9421 cttgccagtt gataatatcc aataaggaaa gaattctccc tttaaggtta cattgggccc
     9481 cttgaaccat tgaaacatcc gataccaatc gtaaatcaca agttaactgc ccttgcagaa
     9541 aatctattga tttctctcca aacaaatcga tgacacctag ataggataag tcaaaaagat
     9601 agtttttctg cttatttaat aataattcag actctatagg gcggaatgtt attaaagacc
     9661 tattattcac taaatgttct aacatagttt tcatatcaag atcagtgatg ttgtttataa
     9721 tacagagtag tgtatcattt ggcctctcaa aatcagatat cattattgaa taattagggt
     9781 tatgttgtat ttatcgtgaa gtaacagagg aaatccattg tggacaatag agaaaaatca
     9841 aggttattat ggaaatgcag gcgaggaatg ctagagcttg atcttgtttt acagaaattc
     9901 atagccaatg agattgatcg acttacagaa aatcaattga aagcatttga taatctatta
     9961 acccacaatg accctaattt atatgcctgg ttaatggggc atgaggaacc cgaaaaagag
    10021 ttgcttgaaa ttgtatcatt catcagaaac tgtgattaaa ccaggtaaat ccaaagtata
    10081 tcttcgatta attacgattc tttatttgtg caccattgga ttagttttat attcttcttt
    10141 acctgtgttg atagaaggcg tcttaatttt tcttatcgtt attcaattta aaaatgacgt
    10201 aacttaccaa agtgcttgcc aaaaagttaa agaaatcaaa tgcttgaaag atcttacgtg
    10261 gtcactggaa acaatagatg gtactactca tcattatgac aaactaaaaa ttcttattca
    10321 taatatgcta tttcaagtta ttcaattatc ttcagataaa aaaaatcaga tgctaattct
    10381 atttaatgat caaataccta ccgatcaatt acgattatta cacctaaaca gcaacaaaaa
    10441 tagtatataa taatacataa tgattaaaat aaatccaagt taacaatgat tagactttat
    10501 cttatcctaa cgtaataaca agcaaaggaa taatgaggaa gctatgcata ttgagacctc
    10561 tttaagtgat gaatctctaa ttgaattagc tcaaaaaggc aacggtttag cttttgataa
    10621 actgtttgag cgttatcagg gcaaaattag acaaatgatt tatttttaca cgcatgactg
    10681 gacaaatgtc aatgatttaa ctcaggaagt tcttatcaaa gtatttaggc atttacctta
    10741 ttttaa
//
LOCUS       DACTJE010000107        10731 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.107, whole genome shotgun sequence.
ACCESSION   DACTJE010000107 DACTJE010000000
VERSION     DACTJE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.107"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            314..853
                     /locus_tag="I9760_002074"
     CDS             314..853
                     /locus_tag="I9760_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HAT5043837.1"
                     /translation="MNTITEDSLTEEQKYMVQVWENHTHYEFDTKNVADTMKTMEDNP
                     YVNNIPTMIGGFGQEEVKQFYSQLFIPQKPDDTEVELISRTVGTHQIVDELIFKFTHN
                     IRMDWILNGIEPTGKRVEIPLIAIVRFHNRKIISEHIYWDQASVLVQLGLLNSDKLPI
                     HGVEATNKIRELISRSLTK"
     gene            907..1755
                     /locus_tag="I9760_002075"
     CDS             907..1755
                     /locus_tag="I9760_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5043838.1"
                     /translation="MIKDYLIETMNQDEVNLAITWAAQEGWNPGIHDATCFYQTDPHG
                     FFAGKLNGKTIAIGSAVIYDDFFAFCGFYIVDKSYRDQGYGLELTKARLVYIGNRNAG
                     IDGVINMLDKYARIGYQLAHNNARYCLNKTIKPISSEPHIVPLNQIDFSQLSSYDRLH
                     FPAPRPQFLKCWINQPQSLALAYLIDGQIKGYGVIRTCHKGFKIGPLFAGTPEIAHQL
                     FSNLVPYAKGQLIFLDIPENNPLAIELVSQYQMKKVFATARMYLKGEPPVKKESIYGI
                     TTFELG"
     gene            1759..2649
                     /locus_tag="I9760_002076"
     CDS             1759..2649
                     /locus_tag="I9760_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAT5043839.1"
                     /translation="MTRDLTGYGSEQINLTWPNKAKIAINFVINYEEGAELTPLNGDT
                     KAEFYGSDFPFVPKGNAERNYSIESIYEYGSRVGIWRLIRLFDKHSIPLTFFVTGYAL
                     ILNPEFSVYLASHNHEVAGHGWRWIDYSKESKATEKKHISRCIDTLQELTGQRPLGWY
                     TGRRSPFTRDLLTQIGGFVYDSDSYADELPYFINKHLVIPYSLDCNDFRFTISPGFVT
                     AQDFYQRLKNTFDYIYQENRLSIMTIGLHPRLSGKPDRCHALNEFLSYIRQFQNIWIA
                     KRIDIALYWLTKHTQIKQDL"
     gene            2865..4079
                     /locus_tag="I9760_002077"
     CDS             2865..4079
                     /locus_tag="I9760_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5043840.1"
                     /translation="MSHLLDLSILKKNRDFTLLYLGQFISFIGTMITSVALPYQIYHL
                     TESTMMVGLLSLAQLLPLLITALLGGVFADRYSRRGLLIISELLLAVGCLFLAFNSKL
                     VNPSVSLIFIIASLMSAITGLHRPAFDSVIQQIVKPEDYKKVGALGSFKFSFCMIIGP
                     AAAGLIIAQYGIVITYLIDLLTFIISLLNLSLMHTIPKPLAKEHPSIIVSLKEGIGFA
                     LRRQELMGSYFVDFFAMVFAMPNALLPAIAQQFGGAKTLGLLYAAPAIGSLIISFVSG
                     WTAKITHDGKAIAIAAALWGVAIIGFGLTTSLWLSLLFLALAGAFDAISGIFRSSLWN
                     HTIPQELRGRLSGIEMISYLSGPRLGDTRAGFVAAGLGIPAALISGGILCIIGVTVCC
                     YALPKFWNYHSK"
     gene            complement(4092..5408)
                     /locus_tag="I9760_002078"
     CDS             complement(4092..5408)
                     /locus_tag="I9760_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1639 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043841.1"
                     /translation="MAGIYLKYYVPVNQIGENMMINPIEYHPKGDKVNSDYFNEYKTK
                     IYERRLSSGLEDLFGDMCGVVVQVQTGDAIPYIKELYSMTPYRYSRSYISDTHKIYCL
                     LNTKNSPAFLVLEPLDPNFKDDITRLNKMYPNSRAKFNARYVGEIYKCKNKDETRNIL
                     VSHDFNFHNPDMTENKFYCNKHIHFTRLSDFTYNCAGYTDDNIYDFDSLELGQRFYLT
                     KEQEAMLDAKDQESHDNGIKDLIKGIDHMATRILAGEREDAILEFLCLSKYYFWGAYN
                     IYDMNSSTNVNRNPHGHDIKSPAKVFTANNTPFMVNSFENLPMPTETFVRNYGRRMHH
                     IAYEVKDGDHSSGKKNIDFVVETLRDKLNIEFLAKVFGACEDSPDLKQIFSKHSIYSI
                     LITEYVERCHHFDGFFTKENVAALTEAASLDETTKQQHKKASIIGD"
     gene            5499..6056
                     /locus_tag="I9760_002079"
     CDS             5499..6056
                     /locus_tag="I9760_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HAT5043842.1"
                     /translation="MEALIVSLGVITLAEIGDKTQLLAFLLAAQFKKPLPIILGILTA
                     TLINHSLAGLIGIWITTLLKPNVLRWILGISFILMAIWTLIPDEIEQDEKKISKYLGV
                     FGATFITFFLSETGDKTQIATIALAAHYSSPILVIAGSTLGMLLADLPAVYFGNLFSH
                     KIPMKIIHAVAACAFLIIGLITILF"
     gene            complement(6074..8044)
                     /locus_tag="I9760_002080"
     CDS             complement(6074..8044)
                     /locus_tag="I9760_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HAT5043843.1"
                     /translation="MKGVCLGLIICLVVFHDGWAGKRLPELDDLYRIQDISEPDLSPD
                     GKWVAYTVSQLNAKDNISTTEVWKVSVDSKSSQRITFAEDSSSSLPKWSPNGKWLAYL
                     SDGGKEGTTQIWIASVQDNKEHQLTHIDGEVSDFTWSPDSNRIAFIAQKSVPSNEEEQ
                     PIVVDRYQFKVDGVGYLGKGRDHLYLVELKIKAITQLTDGNHDEYLPAWSPDGKYIAF
                     VTKRGKDPDRHFNYDIYLIEPKSGAKERQLTHFSGSDMNPDYDSNLSWSPDSSKIAYL
                     RSNEHQWTYYSPNQLAIIDINTKDEKIIAPRDNWYYKPKWSQDGKFIYALIEESRNTY
                     LNRIDTHSGQIKKLTGGLRYDMDYSLAKGKIILLTTDDTHPSELFILNSGLIPLTQHN
                     KKLLDEVKFQPAEDIQCNSFDGTRVDGLLMKPAEYQSGILFPGFVYLHGGPVYQFSHE
                     FNFDMQWLVANNYVVIAPNPRGSSGRGFHYAKAIFADWGNLDVKDVLASVDYVIGKGM
                     VDPNRLGIGGWSYGGMLTNYVIATDSRFKAAISGAGAANILAGYGVDQYTPEYELELG
                     KPWTNPKLYLKLSYPFLKANNIKTPTLFLCSGLDVNVPCVGSEQLYQSLKSLDVPTQL
                     VIYPNEYHTLEKPSFVIDRLKRYTNWLDAYVK"
     gene            complement(8254..9507)
                     /gene="sidB"
                     /locus_tag="I9760_002081"
     CDS             complement(8254..9507)
                     /gene="sidB"
                     /locus_tag="I9760_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidB"
                     /protein_id="HAT5043844.1"
                     /translation="MAKIYNAPKPKYSGWEWFKFIAIRTVFPPVLLWDLIKIGANKLL
                     GEWVSGLVLPAQNENFDDLAISDDTVSNYNEDDLICEKHDVITHDGAHLDTFEVRHRS
                     QESIDPKYQKYIINLVGNGMCYEHIIDDIKEDSKALKANVIGFNLRGVGQSTGKAKSS
                     EDLVADGIAQVQRLLDQGVSPQNITLKGHSLGAGVASLVAQHFHQLGQPINLFNSRSF
                     STITNFLVGHMRLERDEIGRAIGHKDSTVGTILGWLAKPFIKFGVALAKWEINAGSAF
                     KSVPEAYKDYIVVRSRKEIRGERIDDAVIPHYASIHKELASERHKKKAEIDEEIANLD
                     DIIRKADPLAKPGLANARDALVQAREKIKSDRKMETDVQYANGHNSDWNALHNRSGKS
                     AQTFFREFVQRTEADHAVKSIPEIN"
     gene            complement(9763..>10731)
                     /gene="thrC"
                     /locus_tag="I9760_002082"
     CDS             complement(9763..>10731)
                     /gene="thrC"
                     /locus_tag="I9760_002082"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAT5043845.1"
                     /translation="TLSFKDLGTQFFSECLLHLIENKSAKVLVATSGDTGAAIAHALD
                     GKRDLEGIILFPKDKLTFRQQSQITCWGENIRALAVNGSFDQCQQLVKLVFAKREHKG
                     ILTTANSMNIARLLPQIIFYAFTSIQLALRHNHSVNFIVPGGNLGNVTACYWAKMLGF
                     PVEQILIANNINSALGEFLLTGAYQAKSVTKTLATAMDVGEPSNLDRLFVLFNNYSEC
                     KKQMNAESVSDEQIKQAILHCYNKYHYIICPHTATAYHRLSTINQDKPWVIVAPGHPA
                     KFEEVLEPLLKKEIPVPEQLKVLLGRKQHYSIIEPNYHALYHFLVE"
BASE COUNT         3266 a         2089 c         1869 g         3507 t
ORIGIN      
        1 aggaataatt tgataaacac tattatccgt tgataaaaca attcggctac ttaaagcttc
       61 atcaatctca ccatgaaaat cgggtatttt ctcaagttgc tgtaaaaata acctgatttt
      121 tctgggtgtt gcggtggaag aggtcaaacg cgggatcata attttttcct agcttttcct
      181 ccattaatct tctcattata gactaccaga taattagggc taaatcataa agacgactac
      241 tattcaatca gaaaaaatag tctttttagt ctaaattaag gatacagaca tctttttaaa
      301 ggattaaaat gatatgaaca caataacaga agattctctg actgaagaac aaaaatacat
      361 ggttcaagta tgggaaaatc atacccatta tgaatttgac actaaaaatg ttgcagatac
      421 tatgaaaacc atggaagaca atccctatgt caataacata cctaccatga taggcggatt
      481 tggacaagaa gaagtcaaac aattttacag ccaattgttt ataccccaaa aaccagatga
      541 tactgaggtt gaactgattt caagaacagt agggactcat caaattgtag atgaactcat
      601 ttttaaattt actcataata tcaggatgga ttggatatta aatggcatcg agcctactgg
      661 aaaacgtgta gaaattcctc taattgcgat tgtcagattt cataatagaa aaatcataag
      721 cgaacatatt tattgggatc aggcatccgt tcttgtacaa ctcggtttac tcaattcaga
      781 taaactaccc attcatggag tagaagccac caataagata agggaactga tatcacgaag
      841 tctgactaag taagaaaaaa cgcttactta taactataat tatttttaaa gaatacaggg
      901 atcgtcatga taaaagatta tcttatagaa accatgaatc aggatgaggt gaatcttgct
      961 ataacgtggg ctgcccagga aggttggaat ccaggaattc atgatgcaac atgcttttat
     1021 caaactgatc ctcatggttt ttttgcaggt aagctgaatg gaaaaacgat agccattggt
     1081 tctgctgtta tctatgatga tttttttgct ttttgcggtt tctatattgt tgataagtcc
     1141 tatagggatc aaggttatgg cttggaatta accaaagcaa ggctagttta tattggtaac
     1201 agaaacgcgg gaattgatgg tgtgatcaac atgctggata aatatgcccg cattggttat
     1261 caattagcac ataataacgc cagatattgt cttaataaga caataaaacc aatatcatct
     1321 gaacctcata ttgttccatt aaatcaaatc gatttttcgc agttatcatc ctatgaccgc
     1381 ttacattttc cagcacctcg tcctcaattt ttaaaatgct ggataaatca gccacaaagc
     1441 ttggctctgg cttatttaat tgatggccaa attaagggtt atggtgttat caggacctgt
     1501 cataaaggat ttaagattgg gccactattt gcgggtactc cggaaattgc acaccaactt
     1561 ttcagtaatt tggtcccata tgctaagggt caattgatat ttctggatat tccagagaat
     1621 aaccctttag caattgagct ggtcagtcaa tatcaaatga agaaggtatt cgctacagca
     1681 cgtatgtatt taaaaggaga acccccggtt aaaaaagaaa gtatttatgg aattactact
     1741 tttgaattag gataaaccat gacccgagat ctcactggat atggatcaga acaaattaat
     1801 ttgacctggc ccaataaggc taaaatcgct attaattttg ttattaatta tgaagaagga
     1861 gcagagttaa ctccacttaa cggtgataca aaggctgaat tttacggaag tgattttcct
     1921 ttcgttccta aaggaaatgc agaaaggaat tatagtatcg aatctattta tgaatacgga
     1981 agtcgagttg ggatttggcg tttaatccgt ctttttgaca agcattccat ccccttgact
     2041 tttttcgtaa caggttatgc tttaatatta aatcctgaat tctcagtcta tcttgcaagt
     2101 cataaccatg aagttgctgg acatggatgg cgatggatag attattcaaa agaaagtaag
     2161 gcaacggaaa aaaaacacat ctctcgctgt attgatacct tgcaggaact cactggacaa
     2221 aggccattgg gctggtacac tggaagaaga agcccattta cccgcgatct gctaactcaa
     2281 attggtgggt ttgtttatga ttcagatagt tacgccgatg aactccccta tttcattaac
     2341 aagcatcttg taatcccata ctcattagac tgtaatgatt ttcgtttcac aatttctccc
     2401 ggctttgtca cggcacagga tttctatcaa cgtcttaaaa atacttttga ttacatttat
     2461 caagaaaatc gattatctat catgactatt ggtttacatc cgcgattaag cggcaaaccc
     2521 gatcgttgcc atgcccttaa tgaatttcta agctatattc gacaatttca aaatatatgg
     2581 atagcaaaga gaatagatat tgcactgtac tggttaacca aacacaccca gattaagcag
     2641 gatttatgaa atacccaatc tcattgttag gaaatgtttt taattcagtc gtttagttta
     2701 tctaagattc ttcgttaaaa cattttttgc cttgatcgtg aggtttttcg taatgctctt
     2761 atagtctgat cttattaatt tctgtactgt ttcatcaaaa gagcttaaaa ttcatatctc
     2821 aaaatcttta taattcaccc cactataatt tacagattgc caatatgagt catttactag
     2881 atttatccat ccttaaaaaa aatcgcgact ttaccttgct ttatttgggc caattcattt
     2941 cattcatagg cacaatgatt accagtgtcg cccttcccta tcaaatttat catttgaccg
     3001 aatcgaccat gatggtagga ttactcagct tggcacaatt actgcctcta ctgattactg
     3061 cattgcttgg tggagtgttt gctgatcgat atagccgacg cggattacta atcattagtg
     3121 aattattact tgctgttggt tgtctttttc tggctttcaa ttccaaactg gttaatccaa
     3181 gtgttagctt gatatttata atagcctcat taatgtccgc cattacaggt ttacataggc
     3241 ctgcttttga tagtgtgata caacaaatcg tgaaaccaga ggattacaag aaagtaggcg
     3301 ctcttggcag cttcaaattc agtttttgta tgattattgg accagctgct gctggcttga
     3361 ttattgcgca atatggtatt gtaatcactt atctcattga tttattaacc tttatcattt
     3421 cacttctcaa tttgagctta atgcacacca tacccaagcc tttagccaaa gagcatccat
     3481 ccattatagt ttctttgaaa gaaggtatag gttttgcttt gcgccgacaa gagctgatgg
     3541 gtagttattt cgttgatttc tttgcgatgg tttttgctat gccaaatgca ttgttgcctg
     3601 ctattgccca acaatttgga ggagctaaaa cactaggact actttatgct gcgccagcta
     3661 tagggtcgct gattatttct tttgttagtg gctggaccgc aaaaattact catgatggaa
     3721 aagcgatcgc aattgctgct gctttgtggg gagtggcaat tatcggattt ggtctcacca
     3781 cttctttgtg gttatctctc ttgtttcttg ctcttgcagg agcatttgat gccattagtg
     3841 gtattttcag aagcagctta tggaatcata ccatcccgca agaattgaga ggccgcctgt
     3901 ctggaattga aatgattagt tatttgagcg gcccaagatt gggtgatacc agagcaggct
     3961 ttgttgcagc aggtttaggc atacctgctg cattaatatc aggcggtatt ttatgtatta
     4021 ttggagtgac tgtatgctgc tatgccttgc ctaaattttg gaattatcat tcaaagtaat
     4081 taaccacttt actaatctcc aataatagag gcttttttat gttgttgctt ggtagtctca
     4141 tccaaactgg ccgcttccgt taatgcggct acattttcct tggtaaaaaa cccatcaaaa
     4201 tggtgacatc tttcaacata ttcagtaatt aatatggaat aaatggagtg tttggagaaa
     4261 atttgcttta aatcaggtga atcctcgcaa gccccaaata ctttagctaa aaattcaata
     4321 tttaatttat ctcgaagagt ttcaacgaca aaatcaatat tctttttacc agaagagtga
     4381 tccccatctt ttacttcata agcaatatga tgcattcttc tcccgtagtt acgcacaaag
     4441 gtttctgtgg gcatagggag attttcaaag gaattcacca tgaacggagt gttgttagca
     4501 gtaaacactt ttgccggcga cttgatgtca tgtccgtgtg gatttctatt tacattggta
     4561 gaggagttca tatcatagat attataagca ccccaaaaat aatatttgga taaacataaa
     4621 aactctaaaa tagcatcctc acgctcacca gccaggattc gagtagccat atggtcaatg
     4681 cctttgatca agtctttgat cccattatcg tggctttcct gatctttcgc gtccagcatc
     4741 gcctcttgtt ctttggttaa ataaaatcgt tgtcccaatt ccagagagtc aaaatcataa
     4801 atattatcat cggtataccc agcgcaattg taggtgaaat ccgataaccg ggtaaaatgt
     4861 atatgtttat tacaatagaa cttgttttca gtcatatcag gattatgaaa attaaaatca
     4921 tgggaaacta atatgttacg cgtctcatcc ttattcttgc atttataaat ctcacctaca
     4981 taacgagcat tgaatttagc tcttgagttg ggatacatct tgtttaatct tgtgatatcg
     5041 tctttaaaat taggatccag gggctcaagc accagaaaag caggagaatt tttggtattg
     5101 agtaaacaat aaattttatg tgtgtcacta atataacttc gagaatatcg ataaggggtc
     5161 attgaataga gttctttaat atatggaata gcatctcccg tttgtacctg aacaactacc
     5221 ccacacatat caccaaatag atcctcaagt ccggatgata aacgcctttc gtaaatcttg
     5281 gttttatatt cattaaaata gtctgaattg actttatccc ctttgggatg gtattcaatt
     5341 ggattaatca tcatgttctc cccaatttga ttcaccggca cataatattt taagtatata
     5401 cctgccaatg aatttttatt tgaattaata actgtttcct ctctttcata gcaaaataga
     5461 ggtataataa tttattttga atccttacat actttccaat ggaagcttta atcgtatcac
     5521 ttggggtgat tacccttgca gaaattggcg ataaaacaca attactcgcc ttccttctgg
     5581 cagcacaatt taaaaagcca ttgccaatta ttttgggaat attgactgct actttgataa
     5641 atcattcttt ggctggactt atcgggatct ggattaccac tttattaaag ccgaatgttt
     5701 tgcgttggat acttggcata tcgtttattc taatggcaat atggacgctg atacccgatg
     5761 aaattgaaca ggatgagaaa aaaatatcca aatatcttgg tgtttttgga gcaacgttta
     5821 tcactttctt tctgtcagaa acaggagata aaacacaaat tgcaaccatt gccctggcag
     5881 cacactacag ctccccgatc ctggttattg ccggttcaac ccttggaatg ttgcttgctg
     5941 atttaccagc tgtttatttt ggaaatttat tttcccataa aattcccatg aaaataattc
     6001 atgctgtagc tgcatgcgct tttttaatta ttggactcat taccatcttg ttttgaccga
     6061 ctaggcagga ttgctattta acataagcat ctagccagtt ggtatatctt tttaatctgt
     6121 cgatgacaaa gcttggtttc tccaaagtat gatattcatt ggggtaaata actaactgtg
     6181 ttggcacatc tagcgatttc aaggattggt ataattgttc tgacccaaca caagggacat
     6241 taacatccaa gccactgcac aaaaataatg taggtgtttt tatattattc gctttaagaa
     6301 aagggtagct taattttaaa tacaatttag gattggtcca aggctttccc aattccagct
     6361 cgtattccgg ggtgtattga tcgacaccat aaccagctaa aatattcgct gctccagcac
     6421 ctgatatagc cgccttgaaa cggctatctg tagctatgac ataattggtt agcattcctc
     6481 cgtagctcca tccgcctatt cccagacgat ttggatcgac cattccctta cctatgacat
     6541 aatcaacaga agccaagacg tcttttacat ccaaattacc ccaatctgca aaaattgctt
     6601 tggcataatg aaatcccctt ccggaactcc ctcttggatt aggagcaata acgacataat
     6661 tattagctac cagccactgc atatcaaagt taaattcatg gctaaattga tagactggtc
     6721 ctccatgcag atagacaaaa ccaggaaata aaatacccga ctggtattca gctggcttca
     6781 ttaataatcc atcaactctt gttccatcaa agctattgca ctgtatgtct tctgcgggtt
     6841 gaaatttaac ttcatccaat agctttttat tatgttgtgt taaaggaatt aatccggaat
     6901 tcaaaataaa taattcagag ggatgtgtat catctgttgt taacaaaata attttccctt
     6961 tggcaagtga ataatccatg tcatagcgta agccgcctgt caattttttt atctggccgg
     7021 aatgagtgtc tatgcgattc aaataggtgt ttctgctttc ttctattaat gcataaataa
     7081 atttgccatc ttgagaccat tttggcttgt aataccaatt atcccgaggt gcaatgattt
     7141 tctcatcttt agtattgata tcaataattg ccaattgatt gggcgaataa taggtccatt
     7201 gatgttcatt actgcgaaga taagctattt ttgaactatc tgggctccat gaaagatttg
     7261 aatcgtaatc aggattcata tcactaccgg agaaatgagt gagttggcgc tcttttgccc
     7321 cagacttggg ttcgatcaaa taaatatcat aattaaaatg cctatccgga tcttttccac
     7381 gctttgtgac aaacgcgatg tattttccat caggtgacca agccggtaaa tattcatcat
     7441 gatttccatc ggtcagttga gtaatagcct taatcttaag ttcaaccaaa tataaatgat
     7501 ctcttccctt accaagataa ccgacaccat caaccttaaa ttgatatcta tctacaacta
     7561 tgggttgttc ttcctcatta gatggaacac tcttctgagc tataaatgca atcctgttag
     7621 aatctggaga ccatgtaaaa tcgctcactt cgccatcaat atgagttaat tgatgttctt
     7681 tattatcctg gacagatgct atccaaattt gtgtcgtacc ctcttttccg ccatcggata
     7741 aataagctaa ccatttacca ttaggactcc atttgggtag cgagcttgat gaatcttctg
     7801 caaaggtaat tcgttgactg gatttactat ccactgatac tttccatacc tcagtcgtgg
     7861 aaatgttatc tttggcattt aattgggata cggtataggc aacccatttt ccatctggtg
     7921 acagatctgg ctcgctgata tcttgtatac gataaagatc atcaagctct ggcaaacgct
     7981 tcccagccca tccatcatga aatacaacaa gacaaataat tagacccagg cataccccct
     8041 tcatgacaat catccttatt atgctgaaaa atcagagatt tatagtgcct taaaaagtat
     8101 agaataaatt tgttatatat gatttttccg gcaatcagca aacaaatttt gagtggaata
     8161 agtacagtct ggtgtcattg atttagaatc gtagctgaaa ttcttcagaa ataaaaaaat
     8221 ccccgtctgt tcaacgagga tcataaaaag gttctaattt atttctggta tactttttac
     8281 ggcatgatcc gcttctgttc tttgaacaaa ctctctaaag aaagtctgcg cgctcttacc
     8341 tgatcgatta tgcaatgcat tccagtctga attatgacca ttagcatatt gtacatcagt
     8401 ctccattttt ctgtcacttt tgattttttc cctagcttgg actaaagcgt ctcttgcatt
     8461 agccaaccct ggtttcgcaa gaggatcagc ttttcgaatg atatcatcca gatttgcaat
     8521 ttcttcatca atctccgcct ttttcttatg ccgctcagaa gccagttctt tatggataga
     8581 tgcataatgt ggaatcacag catcatcgat acgttcgccc cttatctctt ttcttgacct
     8641 aaccactatg taatctttat atgcttcagg aacactcttg aatgcactgc ctgcattaat
     8701 ttcccattta gctaaagcaa caccgaattt aatgaagggc ttagccaacc atccaagtat
     8761 tgtccctact gtgctgtctt tatgacctat tgctcgacca atttcatcac gctctaaacg
     8821 catatgccct actaagaaat tagtaatagt agagaaagaa cggctattaa atagattaat
     8881 tggctgtcca agttgatgaa aatgttgcgc taccaaggaa gccacgcctg caccaagaga
     8941 atgacctttc agggtaatat tttgtggcga aacaccttga tctaatagtc tttgcacttg
     9001 tgcaatacca tctgcaacca aatcctcact tgatttggct ttgcctgtac tttgaccaac
     9061 accacgaaga ttaaaaccaa taacattggc cttaagagcc ttcgaatctt cttttatatc
     9121 gtcaatgata tgttcataac acataccatt ccctaccaaa ttgataatat atttttggta
     9181 tttgggatca atactttctt gtgacctatg ccttacttca aatgtatcta aatgcgctcc
     9241 atcatgagta attacatcat gtttctcaca aatcagatcg tcttcattat aattacttac
     9301 agtatcatca ctaatagcta aatcatcaaa attttcattt tgtgctggca gcactaaacc
     9361 actaacccat tcaccaagca gtttattggc cccaattttt attaaatccc ataataaaac
     9421 agggggaaaa accgttcgaa ttgcaataaa tttaaaccat tcccaaccac tatatttagg
     9481 ttttggtgca ttataaattt tagccatgag tccaatcctg tttacataaa attgttcata
     9541 ttatatacag ttaatattaa tttaacctta agctgaatat tataaactta aaaaattgtc
     9601 ttaatctgtg aagtaaaaat aaataaagga tgcgtgatcg ctcaatattc tatcattatg
     9661 ctgacttagc ttatgagttt gagccaatca aagacgctgg tgataattaa ccaatgctat
     9721 tatgttatat gccatcacaa caatttgcct ctgtaatggc cttcattcaa ctaagaaatg
     9781 atacaaagca tgatagtttg gctctatgat tgaataatgt tgttttctgc ctaaaaggac
     9841 cttcagctgt tccgggacag gaatttcttt tttcaatagt ggttctaata cctcttcaaa
     9901 tttggccggg tgccctggtg ccacaataac ccagggtttg tcctgattga ttgtacttag
     9961 acgatgataa gctgtggcgg tatgagggca aattatataa tgatatttat tgtaacaatg
    10021 taggatagct tgcttaattt gctcatcact aacagattct gcattcatct gttttttaca
    10081 ttcagaataa ttattaaata aaacgaataa gcggtccaaa ttgcttggct cacctacatc
    10141 catcgctgtt gccaatgttt tagtaaccga tttggcttga taagctccag ttaacaaaaa
    10201 ttcgcccagt gcactattaa tattattagc tattaaaatt tgctcaaccg gaaaacccag
    10261 catttttgcc caataacaag ccgtaacatt ccccaggttt ccgcctggaa ctataaaatt
    10321 aacagaatga ttatgtctta atgccaattg aatgctggta aaagcataga aaataatctg
    10381 tggtaataat ctggctatat tcatgctatt ggctgtagtc aatattcctt tatgttctct
    10441 tttggcaaat accagcttca ccagctgttg acattgatca aaactgccat taacagccaa
    10501 tgcacgaata ttctctcccc aacaagtaat ttgagattgt tgtctgaaag taagtttatc
    10561 tttaggaaat aaaatgattc cttccagatc tcttttccca tccaaagcat gagcaatcgc
    10621 agcgccagta tcaccagatg ttgcgaccag aactttggcg ctcttgtttt ctatcagatg
    10681 caataaacat tcactgaaaa attgagttcc taaatcttta aaagacaggg t
//
LOCUS       DACTJE010000108        10706 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.108, whole genome shotgun sequence.
ACCESSION   DACTJE010000108 DACTJE010000000
VERSION     DACTJE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.108"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(93..1145)
                     /locus_tag="I9760_002083"
     CDS             complement(93..1145)
                     /locus_tag="I9760_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HAT5043846.1"
                     /translation="MHHLKLAERLGIQFPIIQAPMAGGATTPELVAAVSNSGGLGSLG
                     AGYMRPDEIRQAIIKIRQLTSKPFAVNLFIPEAHHATPEQIQKACDDINRCCTELNIE
                     IIPVTKPYSQSFVDQMQILIEEKIPVFSYAFGTLEPMWTKQLKKNGTFLIGTATSIHE
                     ARILEASGIDAIVAQGSEAGGHRGTFIGNAEDALIQLSELIPQLVETIRIPAIAAGGI
                     MNGKGIVSAINAGASGVQMGTAFLSCFEAGITYKYKQTLLSQQQDNTVLTRAFSGKLA
                     RGIRNKFIICMDNRKINILDYPIQNALTQMMRQKAREKDNIDFMSLWAGQSAHLCRSM
                     SASDLISTLVIEAEIR"
     gene            complement(1123..1734)
                     /locus_tag="I9760_002084"
     CDS             complement(1123..1734)
                     /locus_tag="I9760_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT5043847.1"
                     /translation="MKKIAPTLKNEIINTAIDIAQDTSWEKVRLIDIAKALNIDLNTI
                     RYFFAEKEQIIDGCFDRADEAMLNASSRPKFDLLTAKEKLHDLLMTWLSTFEGQQKVI
                     RQMIFHKLEPGHLHLQLPAIKRISQTVQWWREAAGLRETFIQRAIQETGLTIIYLATF
                     SYWLLDNSTEHLATRLFLENKLNMAKNLSNFWGFTYYAPSKIS"
     gene            1904..2806
                     /locus_tag="I9760_002085"
     CDS             1904..2806
                     /locus_tag="I9760_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HAT5043848.1"
                     /translation="MKKIILYLFVAIGVYAVLLFAYNMLINRAGQTLYGGAPAMGNAY
                     PTVEKVNAVPGGSGDSNNNPSASEQVMKGMIIRNANITLEVNDPTKVIDEIMQMAERE
                     GGYVVNSNLRQDSFYVGGNIAQISIRIPSNRLNNVLQNLKSLAVKVIEESITSEDITR
                     QYVDLESELKNYRVAKDQLNKIMQGAKNTSDVLAVYQQLSETQRKIDVIEGQIKYYKE
                     SIAYSLVTIYLQIDPAIKNTQNSTWQISEVVMKSYQALIDNLRQFTYGVIEFVILFVP
                     MILLWGIIALFVFWIGRVIYSRFK"
     gene            complement(2895..3092)
                     /locus_tag="I9760_002086"
     CDS             complement(2895..3092)
                     /locus_tag="I9760_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043849.1"
                     /translation="MLKTLGGATRTAALDTARATATTTVVLGTMYLWDRVAKNYGLYD
                     KENSKPINLDLTNDEYMRPKV"
     gene            complement(3340..4308)
                     /locus_tag="I9760_002087"
     CDS             complement(3340..4308)
                     /locus_tag="I9760_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1137 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043850.1"
                     /translation="MIQRGFTMQERREKQGNNSPYLLTPYEMANLVFKTGAISFTVSA
                     GTQPFQYLLNKLQFSQSGTPSGLSGGLFRGMYRGFLPYAIAGQKRGAVAVTHKQTNKV
                     TEEEEFEAPFRQRWWGTIFFSQADLLVSNGLSGKARLQNVGVINAENFKWSLSNFWKL
                     TSVNWGSRSFAGGVNFALIGFAGDYVSSFYKFDKDLYNKILGGATSGVIATLFTTAPN
                     AYADSKLLQTKVAENNRLITVSPYTMFGQMKSHVKAVGLKEAFMTFFKVSYLQQVAVR
                     APQAAITFALIFGMDEYMGPQPLKKVWPGRVEELESENPSPSPTKK"
     gene            complement(4568..5590)
                     /locus_tag="I9760_002088"
     CDS             complement(4568..5590)
                     /locus_tag="I9760_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT5043851.1"
                     /translation="MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKIIHQFEQESGI
                     KVNFSTYDSNETMYAKLRASQKTIYDVILPSAYFVERMQKQGMLQLLDHKLLPNLHNL
                     DEQFVKNDYDPGNRYSIPIIWGATGIFFNHNSVSNPPKNWQGLWDKKWRNQLMLLDDS
                     REIFAIALMSLGFDPNDTNPNHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDAFIGS
                     AWNGDAFKAHTENKAIEFIYPQDGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSA
                     QIALKEGHAITNAAGRALLPLPIKNNPVVYPPDKTLKKGYFQRDIGEETLALYNQYWE
                     QLKLAF"
     gene            complement(5587..6354)
                     /locus_tag="I9760_002089"
     CDS             complement(5587..6354)
                     /locus_tag="I9760_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAT5043852.1"
                     /translation="MKTMTQRLFLSLVYAFLYIPIAILVLYSVNDAKFSLQWHGFSLR
                     WYKELFHDRGLWSAVFNSITLGLTSSLIATTMGLLASINLFLFRTRHRQILYTLLLLL
                     IIIPDIVLGVALLIFFNITQVPLGFLSLLVAHITFSIPFVILTINSRINTLNPNIYFG
                     ALDLGASRYFALTRVLLPLLWPAVLSAFLLSFTLSFDDVIISYFVAGPDFTILPLTIY
                     SLVRAGVTPELNALCSITFVLSMILVIISHRLSGKLI"
     gene            complement(6351..7175)
                     /locus_tag="I9760_002090"
     CDS             complement(6351..7175)
                     /locus_tag="I9760_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAT5043853.1"
                     /translation="MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPL
                     TLENYFDLFTPVFAKILFRSLLIACVTTLLCLLIAYPFSYLMIKSKHQSILLLLIIIP
                     FWTSSLIRTYALIAILKYKGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMV
                     LPIFTNMERFDFRLIEAAKDLGANKWSIFTRVFLPNTAPGVLSGCLLVLLPAMTLFYI
                     PNVLGGARSILLGNLIQNQFLVLSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR"
     gene            complement(7165..8259)
                     /gene="potA"
                     /locus_tag="I9760_002091"
     CDS             complement(7165..8259)
                     /gene="potA"
                     /locus_tag="I9760_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAT5043854.1"
                     /translation="MTTPLIEIRQIYKSYGNTPILNNVSLNVNHGEFLTLLGPSGCGK
                     TTLLRLISGFEQPTQGEIFINGQCVNQLPPQKRDVHTVFQSYALFPHLSVFENVAFAL
                     RCKKTPNQEIRKRVFDALKLVQLESLAERNVKQLSGGQQQRVAIARAIINRPQVLLLD
                     EPLSSLDYRLRKAMQSELKQLQKTLNMTFIFVTHDQEEALSMSDRIVVFNHGHIEQIG
                     TPKAVYETPANLHVAMFIGEANIFDIQVHTVKDQDIITNIEGIQLSCKNTGNYQVNDR
                     LHLIVRPEDIRVWSLSEVEKTEGMLPGRIVDIIYKGSTVDLKVELSSGKIINASEFFD
                     EDDDKLEYTLHETVWVQWLPGWEVLLPYEG"
     gene            complement(8489..8719)
                     /locus_tag="I9760_002092"
     CDS             complement(8489..8719)
                     /locus_tag="I9760_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAT5043855.1"
                     /translation="MGEKQYVVYILASKAYGTLYTRITSNLVQRIYQHKKGLTEGFTK
                     RYNVHRLVYYEIHTDVYEAITREKCIKKWNRQ"
     gene            complement(8919..9803)
                     /locus_tag="I9760_002093"
     CDS             complement(8919..9803)
                     /locus_tag="I9760_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAT5043856.1"
                     /translation="MTKTDKKIVVVTGCSKTSGVGYNLVCEMLKRGHQVIATVRNLET
                     SQINKANLPNSSNLDVKILDLCDENSIDRFIKEVLSQYQYIDALVNNAANVAIGPVES
                     ATTQDVLTTFQTKVFGPLSLIRGFIPVMRERKKGLLTTTSTIFTSVPVSIPGVAIYFS
                     ALAAFERLQESLAIELAPWNIKVINFQPGPITTELTRFDGSRADIVEKYYKNFTEQAY
                     QWFHQNTVFQNAAEVAKVFAEVIDMENPDLCYQSNLFGKQFVAKNKKDTTGREGMDKL
                     LEHFKNLPHDNPDDWKIQ"
     gene            complement(10150..10656)
                     /gene="cegC3"
                     /locus_tag="I9760_002094"
     CDS             complement(10150..10656)
                     /gene="cegC3"
                     /locus_tag="I9760_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC3"
                     /protein_id="HAT5043857.1"
                     /translation="MDLIFSLGVMIMPLTPPASPKTERRSEEDNQQTNQQGNEQGNQQ
                     PRSSILTLFGFTPNALVMPCPHGHGHFHIMDIQADDTLVFATMNGSPEIMSLLALQFL
                     MQNVNLNLDDMLAAETDAKEAEEKEEQELSSSESVNEEKEEAEFSSSESENEEKEEEN
                     EESSRFTM"
BASE COUNT         3309 a         1954 c         2051 g         3392 t
ORIGIN      
        1 ttagtttatc taaactccct cattaaaaac attttttgcc tcgatcttaa ggttttttgt
       61 aagtcctgtt tttgtaatca ttcacctctc ttttacctaa tttctgcctc aataactaaa
      121 gtacttatta agtcacttgc cgacatagag cggcataaat gagccgattg tccagcccat
      181 aacgacataa aatcaatatt atccttttct ctggcttttt gtctcatcat ctgggttaat
      241 gcattttgaa tggggtagtc gaggatattt attttccgat tatccatgca aataatgaat
      301 ttatttcgaa ttccacgagc aagctttccc gaaaaagcac gtgttagtac agtattgtct
      361 tgttgctgcg ataatagggt ttgtttgtat ttataagtga tgccagcttc aaaacaactt
      421 aaaaaggcgg tccccatttg cacgccagag gcacctgcat ttattgcaga aactatgcct
      481 ttaccattca tgattccccc ggcggctatc gctggtattc gtattgtttc tactaattgg
      541 ggtatcagct cagatagttg aatcaaagca tcttccgcat taccgataaa agtacctcga
      601 tgtcctcctg cttcacttcc ctgtgcaaca atggcatcaa tcccacttgc ttcaagtatt
      661 cttgcttcgt gtatagaggt tgccgttcct atcagaaagg ttccattttt cttcaattgt
      721 tttgtccaca ttggttctaa tgtaccgaat gcatagctaa aaacagggat tttttcttca
      781 atcaggattt gcatttggtc aacaaaagat tgggaatagg gcttagtcac gggaatgatt
      841 tcaatgttta actcagtgca gcatcgattg atatcatcac aagctttttg aatttgttct
      901 ggtgttgcat gatgcgcttc aggtataaaa agattaactg caaagggttt acttgttaat
      961 tggcgaattt ttataatggc ttgtctgatc tcatcaggtc tcatataccc tgcgcccaga
     1021 gagcccaaac caccactgtt agaaaccgct gcaaccaact caggagtagt agctcctccg
     1081 gccataggtg cttgaatgat aggaaattga ataccaagtc tctcagctaa ttttagatgg
     1141 tgcatagtaa gtaaaacccc aaaaattgga taaatttttt gccatattta atttgttttc
     1201 caaaaaaaga cgagttgcaa gatgctctgt ggagttatct aataaccaat aactaaaggt
     1261 ggccagataa atgatggtta atccggtttc ctggatggca cgttgaataa aggtttctcg
     1321 aagaccagct gcttcacgcc accattgaac agtttggctg atgcgtttga ttgcaggcaa
     1381 ttgaagatga agatgaccag gctctaattt atgaaatatc atttgccgga ttactttttg
     1441 ctggccttcg aaagtactta accaagtcat taataaatca tgcagttttt ctttggcagt
     1501 gagtaaatca aattttggtc tggaggacgc attgagcatg gcttcatcag cacgatcaaa
     1561 acagccatca atgatttgct ctttttccgc aaaaaaatat cggatcgtat ttaaatcgat
     1621 atttaatgct ttagcgatat caatcagcct cactttttcc caagaggtat cctgggcaat
     1681 gtctattgct gtattgatga tctcattttt tagtgttggt gctatttttt tcatgaaata
     1741 cctccctgtc tcttattagt ttattactaa ttttatgatc aggattaatt tttgatggtt
     1801 ttggtagtta aattaactat aagttaaatc aaaaaatgct attggttatt tcaattagtc
     1861 tatgccacac ttatttttat aatatcggac tattgaatta aaaatgaaga aaatcatttt
     1921 atacttgttt gtcgctattg gagtttacgc tgtccttcta tttgcgtaca acatgctgat
     1981 aaatcgagcc ggacaaacat tatatggggg cgctccagca atggggaatg cctatcccac
     2041 tgttgaaaaa gtcaatgcgg taccaggtgg ttctggtgat agcaacaata atccttctgc
     2101 ttcagagcaa gtcatgaaag gaatgataat tcgtaatgcg aatatcactc tggaagtcaa
     2161 tgatccgact aaagttattg atgaaattat gcagatggct gagcgagagg gaggatatgt
     2221 agtaaactct aacttaagac aggatagttt ctatgttggt gggaatatcg ctcaaatcag
     2281 cattagaata ccaagtaatc gtttaaacaa tgtattgcaa aatttaaaat ctttggctgt
     2341 taaagtaatt gaggaatcga tcacgagtga ggacattacc cggcaatacg ttgatcttga
     2401 aagtgagctg aaaaattaca gggttgctaa agatcaatta aataaaatta tgcaaggtgc
     2461 aaaaaatact tctgatgtac ttgctgtcta tcaacaactg agtgaaacgc agagaaaaat
     2521 tgatgtgata gaagggcaga ttaagtatta caaggaatct atcgcatatt cactagttac
     2581 aatttacctg cagattgatc ctgcgataaa aaatacacaa aattccacat ggcaaatatc
     2641 tgaagttgtg atgaaatctt accaggcttt aatagataat ttacggcagt ttacttatgg
     2701 agtcatcgaa tttgttatct tgtttgtccc aatgatctta ctgtggggaa ttattgcttt
     2761 attcgtattt tggataggta gggtaattta ttctcgtttt aaataataga atttgcagcg
     2821 tatttcagat tttgatttaa gtgagagcta aatactccca acaggatagt tgggagcaaa
     2881 aataatcttt tagtttaaac tttagggcgc atatattcgt cattagtcag atcaaggttt
     2941 attggcttgg aattttcctt gtcatatagt ccataatttt tagctactct gtcccagagg
     3001 tacatagtgc ctagaacaac cgttgttgtt gctgttgctc tcgcagtatc aagagccgct
     3061 gtcctggttg ctccgcctaa ggtctttaac atacatttcc caaagaatga taacatattt
     3121 accgtcctga ttagttaata ataatcctgc taattattca tcatttattc taaatgaatt
     3181 tctgcctaat gcaatacatt cccctgtttg tatgattaag atcaaatcaa tcatatcttt
     3241 ttaacgtcat gtagattaaa taagtacagt ttatgcagta taacgtattg ttgtttttta
     3301 aggcataatg atgaaaaata acatccttgt tactcttgat tatttcttgg taggggatgg
     3361 ggatggattc tctgactcaa gctcttcgac tcgtccaggc caaacctttt tcaaaggttg
     3421 aggacccata tattcatcca taccaaaaat gagggcaaaa gtgatggctg cttgaggagc
     3481 tcttaccgct acttgctgca aatagctaac tttgaaaaag gtcatgaagg cttccttaag
     3541 accaaccgct tttacatgtg atttcatttg accaaacatg gtatagggtg acacagtaat
     3601 taaacggtta ttttcagcca ctttagtttg caacaattta ctatccgcat aagcattagg
     3661 ggcggttgta aataatgtcg caatgacgcc agaagtagcg ccgcctaata ttttgttata
     3721 caagtcttta tcaaacttat aaaatgaact gacatagtct cctgcaaatc ctatcaatgc
     3781 gaaattgacc ccaccggcaa aagaacgaga accccagttg actgaagtta atttccaaaa
     3841 gttggaaagg gaccatttaa agttttcagc attaataacg ccgacatttt gcagtcttgc
     3901 ctttccactt aaaccattac taaccaatag atcagcctga gaaaagaata tggttcccca
     3961 ccaacgttga cggaatgggg cttcgaattc ttcctcttct gttactttgt tagtttgttt
     4021 atgggttaca gccacagcac cgcgtttttg acctgcaata gcataaggaa ggaagccacg
     4081 gtacatgcct cggaaaagtc ctccgctcaa tccagatgga gttccagatt gtgaaaattg
     4141 caatttgttt agcaagtatt gaaatggttg agttcctgca gaaacagtga aagaaatagc
     4201 tcctgtttta aaaactaaat tggccatttc atagggcgtc aacagatagg gtgaattatt
     4261 accctgtttc tctctacgtt cttgcattgt aaatcctctt tgaatcactt aaatcaatgt
     4321 gctgtaatgg taacaattaa ttattaagca aatattaact gatgtgaaaa aaaaatatta
     4381 agtatgagaa taatatgtaa aaagtgagac aaataaaaac ataaaataga ttgaatttat
     4441 acaaattaag cataattatt ttctttttgt atacaaaata tgcaagcaac cttgctttct
     4501 gaattttaat tcgttgagtt aatgaagtac ttcatctgga tatttagcgc tacttcacaa
     4561 cagaaaacta aaaagcaagt tttaactgtt cccaatattg attataaagc gctagtgttt
     4621 cttcgccgat gtcgcgttgg aaataaccct tctttagtgt tttgtctggc ggataaacca
     4681 caggattatt ttttataggc aatggcaata aagctcttcc tgcggcattg gtaattgcat
     4741 gtccttcctt tagtgcaatt tgagcgcttg tttccggttt cagaataaaa ttaatgaatt
     4801 gatacgcctc tttcaggtgt ggggggtttt tagggatagc caaacaatct acccaaatca
     4861 caaaaccatc ttgaggataa ataaactcaa tggctttgtt ttccgtatgt gctttgaagg
     4921 catcgccatt ccatgctgat ccaataaaag catcctcatc tatcatgatg gcttgaatac
     4981 tatcactggc aaataactta atattaggta ccaattgcaa taagtgttca taggctgctt
     5041 ttatgtggtt tggattggta tcattgggat caaaccctaa actcatcaga gctatggcaa
     5101 atatttcgcg tgaatcatca agtaacatta gctgattacg ccacttttta tcccataagc
     5161 cttgccagtt ttttggcggg ttcgatacgg aattatgatt aaagaaaatc ccggttgctc
     5221 cccagatgat gggtatacta taacggttac ccggatcata atcattttta acaaattgtt
     5281 catccagatt atgaagattc ggaagcagtt tatgatcgag taattgcaac atgccttgtt
     5341 tttgcatgcg ctctacaaaa taagcggaag ggaggatgac atcgtaaatg gtcttttgac
     5401 tggctcttag cttggcatac atggtttcat tgctatcata agtcgaaaaa ttgactttaa
     5461 ttcctgactc ttgttcgaat tgatgaatta tttttttagg gatttcacct ccccaggcat
     5521 agacattgac gactggattg gagaatgcct taaatgtaag aagagcgagc agcaatgaaa
     5581 aaattttcat atcaatttac ctgacaagcg atgagagata ataacgagaa tcatcgataa
     5641 aacgaacgta attgaacaca atgcattgag ttcaggcgtg acgccagcgc ggactaaaga
     5701 gtagatagtc aaaggcaaga tagtaaaatc agggcctgcc acaaaatagc tgataatgac
     5761 gtcatcaaat gacaaagtga aacttaataa aaaagcgctt aaaaccgcag gccacaataa
     5821 aggaagcaag actcttgtta aagcaaaata acgactggcg ccaagatcga gggcgccaaa
     5881 ataaatgttg ggatttaaag tgtttatacg tgaattaatg gtcaagataa caaaaggaat
     5941 ggagaatgta atatgggcaa ctaaaaggct caagaagcca agcggtactt gggtaatatt
     6001 aaagaaaatt aataaggcaa cccccaggac tatgtcagga ataataataa gtaatagtaa
     6061 taaggtatat aggatctggc ggtgtcttgt acgaaataaa aataagttga ttgaggccaa
     6121 tagccccatt gttgtagcga taagagagga tgttaggccc agggtgatag aattgaaaac
     6181 agcagaccac aagcctcgat catgaaatag ttctttgtac catcgcaaag aaaacccatg
     6241 ccattgtaat gaaaatttgg catcatttac tgaatagagc accagtattg caatggggat
     6301 ataaagaaaa gcataaacta aagaaagaaa caggcgctgt gtcattgttt tcatcgaatg
     6361 acctcattgt tctgtcgccg aaaaatgaac attaaaataa gcagaacgga agttaatagc
     6421 acactggtag cggctccctg aggccaatcg ctcaggacaa ggaattgatt ttggattaaa
     6481 tttcccaata aaatggaacg agcgccacct aatacattag gaatgtaaaa taaagtcatt
     6541 gctggtaaca agaccagcaa acaacctgat aagactccag gggctgtgtt aggcaaaaat
     6601 actcgtgtaa atatagacca cttgtttgcc cctaaatctt tagctgcctc aattaacctg
     6661 aaatcgaacc gttccatatt agtaaaaatg ggtaggacca tgaaagggaa aagattgtaa
     6721 accaagccaa taacgaccgc aaaattggta tagagtagtg acaagggaac atcaatcaaa
     6781 tgcaatttca gcaagagggc atttatcacg cctttgtatt taaggatagc aatcaaagca
     6841 taagttctta ttaatgagct tgtccagaaa ggtatgataa tcaacagcaa taaaatagat
     6901 tggtgttttg atttaatcat aagataacta aagggatagg cgatgagcag gcaaagaagg
     6961 gtggtgacac aggctatcag caaggatcgg aaaaggattt ttgcaaaaac aggagtaaac
     7021 aagtcaaaat aattttccaa tgtcaatggc aaggtaacaa gatgaatact gtcttgggat
     7081 aaaaagctgg caaccagcac cattaacaaa ggaatgaagc caaaaataat tagccaaaaa
     7141 tacaataaat acagtgcaga tgatttagcc ttcataaggt aataaaacct cccagccagg
     7201 caaccattgt acccaaacgg tttcatggag agtgtattcc agcttatcat cgtcttcatc
     7261 aaaaaactcc gaagcattga tgattttgcc tgatgacaat tccactttga gatcaaccgt
     7321 agagccttta taaatgatat caacaatacg accaggcaac attccttctg ttttttctac
     7381 ttcggataag ctccaaactc gaatatcctc tggacgaacg atcaaatgaa gccggtcgtt
     7441 gacttgataa ttgccagtgt ttttacagga tagctggatt ccttcaatgt tcgttatgat
     7501 gtcttgatcc tttactgtgt gaacctgaat atcaaatata ttcgcttcgc caataaacat
     7561 ggcaacatgc aggttggcag gggtttcata gacagccttg ggagtaccaa tttgttcaat
     7621 gtggccatga ttaaagacga caatacggtc agacattgat aaggcttctt cctgatcatg
     7681 cgttacaaaa ataaacgtca tattcaaggt tttttgtaat tgtttcaatt cggattgcat
     7741 ggctttccta agcctgtagt caagggaact caatggctca tctaacaaca gtacttgagg
     7801 tctattgatg attgctcgag caatcgcaac acgttgttgt tggcctccac ttaactgttt
     7861 cacatttctt tcagctaacg attcaagttg tacaagtttt aacgcatcaa agactcgctt
     7921 cctgatttcc tggttgggcg tttttttgca gcgcaaggca aatgcgacat tttcaaaaac
     7981 ggataaatga gggaaaaggg cataactttg aaaaacagta tgcacatccc gtttttgagg
     8041 tggtaattga ttaacacatt gaccattaat gaaaatctca ccttgcgttg gttgttcaaa
     8101 accagatata agccgaagaa gggttgtttt tccacaacct gaagggccca gtaaggttaa
     8161 aaattcaccg tgattgacgt ttagcgaaac attattcaaa attggtgtat tgccgtatga
     8221 tttataaatc tgacggattt caatgagcgg tgttgtcatc tgctttatta aataaaaact
     8281 gcaattatgt gtgctgtgac ttatcttgtc cagtcaatca caaactttgt tcccaatatt
     8341 aaaaaattag tcaactattg tctatttttg tgttttgtac atgagcaaaa aaatcagtca
     8401 tgcccgcgta ggcgggcaac catctatcaa cataatccaa tgctcaggtc aagccgctga
     8461 ggattttttt gttcgatcaa gtttattttc attgcctgtt ccatttctta atacattttt
     8521 ctctggttat tgcttcataa acatcggtgt gaatttcata ataaactaac cgatggacgt
     8581 tgtatctttt agtaaatcct tcggtcaaac cttttttgtg ctgatatatt cgttgcacaa
     8641 gattactggt aatcctggta taaagggtcc catatgcctt actagctaat atataaacaa
     8701 catattgttt ctctcccatt attcatcctg gttggttgct atatcctgcc gcaattttgc
     8761 gatcgagatt atatgatttt tattgtatat cagcgagtta cttcatcttg tttaggtggt
     8821 aagcgaggaa tggttgcccg cctgcgcggg catgatcgat tttttgctta tgcacaaaga
     8881 tttcgcggcg gatagtatga ctttatccag taaatcattt attgaatttt ccaatcatca
     8941 ggattgtcat ggggtaaatt tttgaaatgc tccagaagct tgtccattcc ttcacgccct
     9001 gttgtatctt ttttgttttt tgcaacaaac tgctttccaa aaagattgct ttgatagcat
     9061 aaatcaggat tctccatatc aataacttca gcaaatacct tggctacttc cgctgcattt
     9121 tgaaaaacag tattctgatg gaaccattga taagcttgtt cagtaaagtt tttatagtat
     9181 ttctcaacaa tatcagcgcg agagccatca aatcgggtca attctgtggt aattggccca
     9241 ggttggaaat taattacttt gatattccat ggggccagct caatcgccaa ggattcttgt
     9301 aatcgttcaa atgctgcgag tgctgaaaaa tagattgcga cacctggaat actaacggga
     9361 acagaggtaa aaatcgtcga tgttgttgtt aataaacctt ttttcctttc tctcattaca
     9421 gggatgaatc ccctgattaa agacaatggg ccaaatactt tggtttgaaa agttgttaat
     9481 acatcttgag tggttgcgct ttcaacaggg ccaatagcca cattagcagc attgttaacc
     9541 aaggcgtcaa tgtattgata ttgacttaag acttctttaa taaagcggtc aattgaattt
     9601 tcatcgcaaa gatccaatat ttttacatcc aaattgctgg agtttgggag gtttgctttg
     9661 ttaatttggc ttgtttcaag atttctcacc gttgcaatga cttgatgccc gcgctttagc
     9721 atttcacata caagattata tcctacgcca gatgtttttg aacaacctgt gaccaccact
     9781 atttttttat ccgtcttcgt catgtcccta tcctaatgat cttttagagt aataaaccga
     9841 gtatagccct tttatttatt cagaacttac gaaaaacccc aatctctttg ttaaaaaggt
     9901 ttttaattcg gtcatttatt attgattttc caaactctta actgattttt tgaaagcaga
     9961 aaaatattgg ttgagaaaat ataggatatt cagtgatata tagtttatta ctgagattag
    10021 ttgctatcgt ttgattattg ggacatttat tctgataaag attgacaata ttgaagacag
    10081 aggaattaaa aaaatcccgt atttacctca ggtaaatacg gggtgtccct tcaaaagagg
    10141 gcaggcatat tacattgtaa aacggcttga ttcttcgttt tcttcttctt tttcttcatt
    10201 ttctgattca gaagatgaaa attctgcttc ttctttttct tcattcactg attcagacga
    10261 tgacagttcc tgttcttctt tttcttctgc ttctttcgca tctgtttctg ctgccagcat
    10321 atcatcaaga tttaagttga cattttgcat caggaattga agcgccaata gactcattat
    10381 ttccggactg ccattcatag tagcaaatac taatgtatca tctgcctgaa tatccataat
    10441 atggaagtga ccgtggccat gagggcatgg catgactaga gcattaggag taaaaccaaa
    10501 aagagtcaat atactgcttc ttggttgttg atttccttgc tcgtttcctt gttgattagt
    10561 ttgttgatta tcttcttcag aacgtctttc agtttttggt gaagcggggg gggttaaagg
    10621 cataatcatt actcctaaac taaaaattaa atccaaaaaa tactgcccaa aaaagataat
    10681 ctcattaaca atctatcttt tcggcg
//
LOCUS       DACTJE010000109        10560 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.109, whole genome shotgun sequence.
ACCESSION   DACTJE010000109 DACTJE010000000
VERSION     DACTJE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.109"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..611
                     /locus_tag="I9760_002095"
     CDS             <1..611
                     /locus_tag="I9760_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HAT5043858.1"
                     /translation="LPGPKGDKGEAGPQGLPGPKGDKGETGAVGPQGMPGPKGEAGDD
                     GQGVPAGGETGQVLAKSNDLDFNTMWVDPANSGIRRQLGDKALGGTVIYVNALGTHGL
                     VVANSDQVNASTWWDAHDFISNPAHFDNEGKLYSDWRLPTRFELNLIYMMRNELGNFL
                     AGNYWSSIEKSSANSWVFNSKTGEIKDLAKNKTAAVRAVRAF"
     gene            complement(635..2491)
                     /gene="uvrC"
                     /locus_tag="I9760_002096"
     CDS             complement(635..2491)
                     /gene="uvrC"
                     /locus_tag="I9760_002096"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAT5043859.1"
                     /translation="MNDLQLSAELALFLTKLPSEPGIYRMLDEEGTVLYVGKAANLKK
                     RVNSYFSKQNTGVKTRALVSQIKSIEISVTRSETEALLLESNLIKALRPKYNVLLRDD
                     KSYPYIHLSNHPDFPRIELYRSKKKPPSGNFFGPYPGVAAVRETIVTIQKIFKIRNCR
                     DSYFKARSRPCLQYQIKRCTAPCVHYISPENYKLSVEDAIRFLQGKCQIILDELAERM
                     KQAVSQLNFEEAAVLRDQIKNLRLIQEQQGVVQLRGDADVIAIEVRPGFACIQCVTIR
                     EGQVLNSQSFFPTVPYAVLDEELDANSLWQQTFEAFIGFYYLDTSERIPDLIITNQSI
                     TESRSLEYILSQRRGKSCKIQINPRGVKSRWMDFAVNNLRISVAEYVSKHSTIRSRYQ
                     ALKQLLALDKNIERMECFDISHTQGEATVASCVVFDTEGPRPSEYRRFNIEGITPGDD
                     YAAMEQAVTRRFKRLIDVQLLPDVLIIDGGKGQVSIVKRVLTSLGVEDITLLGVSKGP
                     SRKAGWEKLILVNENREFVLPEDSKALHLLQHIRDEAHRFAITAHRKKRQKTRVESTL
                     ESIEGVGAKRRQALLQRFGGLRELAKAPLEEICKVQGISEQLAKRIYEHFHP"
     gene            complement(2503..3162)
                     /gene="uvrY"
                     /locus_tag="I9760_002097"
     CDS             complement(2503..3162)
                     /gene="uvrY"
                     /locus_tag="I9760_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAT5043860.1"
                     /translation="MIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVK
                     THSPDVVLLDMKMPGIDGWEVTRRLKKTNPNIKVIAVTAICSDPLPTRVLQLGAMGYL
                     TKESGAEEMAAAIRKVAKGEKYLSAEIAQKMAINSLQESQDSPFDLLSEREMQVMLMI
                     TSGMNVQDIADRLFLSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQD
                     D"
     gene            complement(3287..4105)
                     /locus_tag="I9760_002098"
     CDS             complement(3287..4105)
                     /locus_tag="I9760_002098"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HAT5043861.1"
                     /translation="MEFSSRYVAHVPDAQGLVDYSAQENRIWNILFERQLKLLPGRAC
                     DEFLSGLQTLGLNSSTIPQLPEVSERLKAKTGWQVAPVAALISAREFFELLAEKYFPA
                     ATFIRSEEELDYVQEPDIFHELFGHCPMLTDRVYAEFVHDYACKVLTFPEQDWPLLQR
                     MFWFTVEFGLIKTPKGLRAYGGGILSSISETVYCVESDIPVRILFDPVVAFRMPYRID
                     QLQPVYFVIDSYQNLYDFVLSDMGKFMDRARELGEFPPYFDVDPDNPNIHIRAC"
     gene            4544..5641
                     /locus_tag="I9760_002099"
     CDS             4544..5641
                     /locus_tag="I9760_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAT5043862.1"
                     /translation="MTIAILSTGDEIIHGDTLNTNAHYIAHALCSEGLPLGLQVACSD
                     KENEIIDSLNFLTQHHDILILIGGLGPTKDDITRFALAKFTKEPLLQHADALDHIEKK
                     ANIAKISLNQGNLQQCLFPANARLFPNPYGTAMGCSYSWKGKVFILLPGPPRECLPMF
                     NQDVLPLLLQTSRHSNKQILRWRVFGLAESEIAQILEEALEGLDCQTGYRLETPYLEF
                     KVRCKRELIEKITSIIDPILAPHIISSLETKASDQLRSLILKNNEPITIIDEITGGLL
                     QSLLVKPENYHLLNFHNLNRTRLYFHCSGLEDYWSQQAPQGTTKLIIHYSNQMQEGRE
                     THQIPYRSAMVVHYAAEWLSFRLFHLINQLH"
     gene            complement(5600..6625)
                     /gene="obgE"
                     /locus_tag="I9760_002100"
     CDS             complement(5600..6625)
                     /gene="obgE"
                     /locus_tag="I9760_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAT5043863.1"
                     /translation="MKFVDEALIKVEAGKGGNGCLSFRREKFIPRGGPDGGDGGDGGS
                     IYFEASSDLNTLIDFRYTRQYKAENGQSGMGGNCTGKKGDDLTIKVPVGTMVYDADTG
                     ELLADISQPGIPVLIAQGGFHGLGNTRYKSSVNRSPRQTTPGSPGESRNLRLELRVLA
                     DVGLLGLPNAGKSTLIRAVSSSKAKVADYPFTTLHPGLGVVRVSPYKSFVMADIPGLI
                     EGAAQGAGLGHRFLKHLSRTCVLLHVIDIAPLDGSDPVADAKAILNELTQYNPDLLNK
                     PRWLVLNKIDMLPDEKEREEKIQSIIKGLEWKDKVFSISAIESKGTQELCYALMQLID
                     EMKESEA"
     gene            complement(6840..7118)
                     /gene="rpmA"
                     /locus_tag="I9760_002101"
     CDS             complement(6840..7118)
                     /gene="rpmA"
                     /locus_tag="I9760_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAT5043864.1"
                     /translation="MAHKKAGGSTRNGRDSNPKYLGVKRFGGQFVNAGEIIVRQRGTR
                     FHPGPGVGCGRDHTLYALVEGLVQFTTKGEKNRKYVTILPEQREEAAS"
     gene            complement(7131..7442)
                     /gene="rplU"
                     /locus_tag="I9760_002102"
     CDS             complement(7131..7442)
                     /gene="rplU"
                     /locus_tag="I9760_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAT5043865.1"
                     /translation="MYAVIKTGGKQYTVKEGDVLKIEMLPENVGNEIKFSEVLMLVDG
                     DKVTCGTPFVAKATVKAEVLDHGRHKKVKIIKFRRRKHHMKQMGHRQYYSQVKITAIG
                     K"
     gene            7685..8344
                     /locus_tag="I9760_002103"
     CDS             7685..8344
                     /locus_tag="I9760_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAT5043866.1"
                     /translation="MSTIQLEAQSRTDMGKGASRRLRRLENKVPAVIYGGSKKPMAIH
                     FSHNKVIKALETESIYSSVFDITVDGKVEHVILKALQRHPYKPIVLHMDLQRVSSKDI
                     LVKLVPVHFINEEQSPGIKAGGIVQHTMTQVEIRCQAKDLPEFIEVDMSKVGMDDVVH
                     LSDLKLPKGVQLTVDVADGSHDAPVVSIHAAKVSSTELEETPEVPASAVPTTDQGESA
                     E"
     gene            8463..9032
                     /gene="pth"
                     /locus_tag="I9760_002104"
     CDS             8463..9032
                     /gene="pth"
                     /locus_tag="I9760_002104"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAT5043867.1"
                     /translation="MVIKLIVGLRNPGSAYEQTRHNAGAWLVTALAQRHNSHFKIDKK
                     MQAELTEIDINNHPCRLVLPLTFMNHSGQTTRIISQFYKIEPGEILIVHDELDLPVGR
                     IKLKTGGGHGGHNGLRDITAQLGTGEFHRLRIGIGHPGHKDLVHQYVLSRPSMHDRQQ
                     IYDAIDRGIAIIPIVLSGDMARAMNQVNA"
     gene            9051..10142
                     /gene="ychF"
                     /locus_tag="I9760_002105"
     CDS             9051..10142
                     /gene="ychF"
                     /locus_tag="I9760_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAT5043868.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNVGIV
                     TVPDSRLDNLSDIVKPQQVLHATMQFVDIAGLVKGASSGEGLGNQFLANIRETDAIAH
                     VVRCFDNSDVVHVEGRVDPLSDIEVINTELALADMETLEKSLLKVGKNSKSGNKEAIF
                     ELKTLEKIKAHLDAGYPVRTLELTPEEEQVSKRLFLLTAKPVLYIANVDDNGYENNPL
                     LDKVQALASQENASIVALCAATEAELVELDEEDRQEFMVDLGLSEPGLNKVIRAGYEL
                     LGLQTYFTAGVKEVRAWTIRKGATAPQAAGVIHTDFEKGFIRAEVISYDDFIRFGGEQ
                     GAKEAGKLRLEGKEYIVCDGDVMHFRFNV"
BASE COUNT         3281 a         2218 c         1983 g         3078 t
ORIGIN      
        1 gattacctgg tccaaaaggt gacaaaggag aagcagggcc gcagggatta ccaggcccta
       61 aaggagacaa aggagaaaca ggagcagtag gtccacaagg tatgccagga cctaaaggcg
      121 aagcaggaga tgacggccaa ggtgtgcctg caggtggtga aacgggtcaa gtccttgcca
      181 aatcaaatga cctcgatttc aataccatgt gggttgatcc ggcaaattcc ggcattaggc
      241 ggcaattagg cgataaagct cttggcggta cagtaattta tgttaatgcg ctaggtaccc
      301 atgggcttgt ggtggcaaac tcagatcaag ttaatgcaag cacatggtgg gatgcacatg
      361 acttcatatc aaatcctgcc cactttgaca atgaaggtaa attatattct gactggagac
      421 tccctacccg ttttgaattg aatttaattt atatgatgcg taatgagctg ggaaattttt
      481 tggcaggtaa ttactggagc tcgattgaaa aatcatcggc aaacagctgg gtatttaatt
      541 ctaaaacagg ggaaattaaa gaccttgcca aaaataaaac ggctgctgta cgtgctgtaa
      601 gagcctttta agcatggcaa aacttcaatt ttgatcaagg gtggaaatgt tcataaattc
      661 gctttgccaa ctgctcgctg attccctgga ccttacaaat ttcttccagt ggcgctttag
      721 ccaactctcg caacccgcca aaacgctgca gcaacgcttg acgtcgtttc gcgccaacac
      781 cctctatact ttcaagagtg gattcaacac gtgtcttttg tcttttcttc cgatgggcag
      841 taatagcaaa acgatgcgct tcatcacgaa tatgttgtaa aagatgaagc gctttggagt
      901 cctctggcaa aacaaattct ctgttttcat taaccagtat caatttctcc catcccgcct
      961 ttcgtgaagg ccccttggat acacctaata aagtaatatc ttccacaccc agagaggtta
     1021 aaactcgttt tacaatagaa acctggcctt tacctccatc aataatcaaa acatctggca
     1081 ataattgaac atcaatcagt cttttaaatc ggcgagtaac tgcctgttcc attgctgcgt
     1141 aatcatcccc tggagtaata ccctcaatgt taaatcgcct gtattcactg gggcgagggc
     1201 cttctgtatc aaatacaaca caagatgcaa cagttgcttc accttgagta tggctaatat
     1261 caaaacactc cattcgctct atatttttat caagcgcaag caattgcttt aatgcctgat
     1321 atcttgatct tatcgtagaa tgtttgctga catattctgc tactgaaatt cgtaaattgt
     1381 tcactgcaaa atccatccat ctcgatttga cacctcttgg attgatttga attttacagg
     1441 atttgcctcg tcgctgggac aaaatatact ctaatgagcg actctctgtt attgattgat
     1501 tagtaattat caaatccgga attctttctg atgtatccaa ataataaaaa ccaataaagg
     1561 cttcaaaagt ctgctgccaa agtgaattgg catccaattc ttcatctaat acagcataag
     1621 gcactgtagg gaaaaagctt tgactattca atacttgtcc ttcacgaata gtcacacact
     1681 gaatacatgc gaaaccagga cgtacttcta ttgcgatcac atcagcatca ccacgtaact
     1741 ggacaactcc ttgttgttct tgaattaagc gcaaattttt tatctgatca cgcaaaacag
     1801 cagcttcttc gaaatttaat tgacttaccg cttgtttcat tcgttcggca agttcatcaa
     1861 gaattatttg gcacttcccc tgtaaaaatc ttatggcgtc ctctacagac aatttgtagt
     1921 tttcaggaga aatataatga acgcaaggcg cggtacaacg ttttatttga tattgtaaac
     1981 aagggcgaga acgagcttta aaatagctgt ctctgcaatt gcgaatttta aatatttttt
     2041 gaatggtaac tatcgtttct cttacagcag ctaccccagg ataggggcca aaaaaattac
     2101 cggagggcgg cttcttttta cttcggtata actcaattct tggaaagtct ggatgatttg
     2161 atagatgaat ataaggatag gatttgtcat cacgcaacaa aacattatat tttggcctta
     2221 acgctttaat taaattactc tctaataata aggcctctgt ctctgatctt gtcacagaaa
     2281 tttctatgga tttgatttgg cttaccagtg ctcgtgtctt aactcctgta ttttgtttac
     2341 taaaatagct gttgactctt tttttaagat tggctgcttt tcccacatat agaacggtgc
     2401 cttcttcgtc taacatgcgg tatatgcccg gttcacttgg gagtttcgtt aaaaataaag
     2461 cgagttcagc ggaaagctgt aagtcattca tgaaattaac atctaatcat cttgtgacaa
     2521 atcattgggg tgctcaataa tgcggtgctt catagccaaa taagtaagct ccacatcatt
     2581 tttaatcccc aatttttcaa acatcctata tcgatagcca ttgatggttt tactacttaa
     2641 aaataatcta tcagcaatgt cttgtacatt catgccgcta gtaatcatca acatgacttg
     2701 catttctctt tcagacaata aatcaaaagg ggaatcctga gactcctgaa gactattaat
     2761 tgccattttt tgtgctatct cggcactaag atatttctca cctttagcca ctttacgaat
     2821 tgcggcagcc atttcttcag cccctgactc tttagtaagg tatcccatag cacctaattg
     2881 caatactcga gtagggagag gatctgagca aatagctgta acagctatga ctttaatatt
     2941 tgggtttgtt ttttttaaac gacgcgtcac ttcccaccca tctatcccag gcattttcat
     3001 atctaataat acgacatcag gggagtgggt ttttacatag gctaaagcct gctcaccact
     3061 ttcagcatct gcaactacat caacatccga catatcttca agcaatcgtc gaatgcccat
     3121 tctaaccaat gcatggtcat caacaattaa tactttaatc aaataatgct ccttgaccaa
     3181 tcagtcaatg aacaaactgt gttggtcgat gttaacaaga ctcgataatc attacaatac
     3241 atagtcactc taatctacaa gtccagctgt cttacaaaat aacaaattaa caagccctta
     3301 tatgaatatt tggattatcc ggatccacat caaaatacgg tggaaattca cctaactctc
     3361 gcgcacgatc catgaattta cccatgtcag aaagcacgaa atcatataaa ttttgatagc
     3421 tgtcaataac gaaataaaca ggttgtagct ggtcaatccg ataaggcatt cgaaaagcca
     3481 ccactggatc aaataaaatt cgcacaggaa tatcactttc cacacaatat accgtttcac
     3541 tgatagaaga taaaattccc ccgccgtatg ctctaagccc tttaggcgtt ttaatcaatc
     3601 caaactctac agtaaaccaa aacattcttt gcaataaagg ccaatcctgt tcaggaaaag
     3661 ttaatacctt acatgcgtaa tcatggacaa attcagcata gactctatcg gttaacatag
     3721 gacaatgacc aaaaagctca tgaaaaatat caggttcttg aacataatcc aattcttctt
     3781 cacttcgaat aaaagtcgcc gcaggaaaat atttttctgc taataattca aaaaattccc
     3841 tggctgaaat taaagcagca actggcgcta cttgccatcc cgttttggcc tttaatcgct
     3901 cacttacttc tggaagttgt ggaatagtcg aggagttaag tcctaaagtc tgtaatccag
     3961 acagaaattc atcacaagct cttcctggca ataacttgag ttgcctctca aataaaatat
     4021 tccaaattct attttcttgt gccgaataat cgactaaacc ctgagcatca gggacatgtg
     4081 cgacataccg gctactaaac tccataatct ctccttcact catctcattc aaaaataaat
     4141 aaccagggat aataatattt atttgtattt agggtcagcc tattaaagtc aacgagaatt
     4201 tttacctaaa agttgcttta ttttgagtat acatcctagc caataccctg ttttagattg
     4261 gcattattat gatgatcact aaactctcta acttgtttta gtaattacct aacagtaaac
     4321 taaatgtcaa catgattacc attatctttt tgagataccc tctaacaatg gaaagcaatg
     4381 agtaactttc cgatatggca aaaattacca actggtgtat gtgaggtaac tggggcatca
     4441 taaaagataa cttaatctgt taacccttat atattaatta ttacttattt tacagattct
     4501 gctatagaat ttattacaga aattacccat tggaatacac aaaatgacaa tagctatctt
     4561 gtctaccgga gatgaaataa tacatggcga tactctaaat accaatgcgc attacattgc
     4621 tcatgccctt tgctctgaag gcttacctct tggattacaa gtggcctgta gcgataagga
     4681 aaatgagatt atagatagcc taaacttcct gacgcaacac catgatattc taattttaat
     4741 tggtgggctt ggcccaacga aggatgatat tactcgcttt gctttggcta aatttaccaa
     4801 agaacctctt cttcagcatg ctgacgctct ggatcatata gaaaaaaaag caaacatagc
     4861 caaaatatca ctgaatcaag gcaatttgca gcaatgcctc tttccagcca atgcacgact
     4921 ttttcctaat ccttatggca cagccatggg atgttcttat tcatggaaag gtaaagtttt
     4981 tattctattg cctgggcctc ctcgtgaatg ccttccaatg tttaatcagg atgtactgcc
     5041 gttattattg caaacatcca ggcatagcaa taaacaaatt ctgcgctggc gagtttttgg
     5101 attggctgaa agtgaaatag ctcaaatcct tgaagaagct ctggaaggtt tagactgtca
     5161 gacaggatac cgattggaaa cgccttatct ggagtttaag gttcgctgca aaagagaatt
     5221 gattgaaaaa ataacatcaa taatagaccc cattttagcc ccgcacatta tatcatcact
     5281 tgaaaccaaa gcatctgatc aattgagaag cctaatctta aagaataatg aaccaataac
     5341 tattattgat gagattactg gaggattatt gcaatcccta ttggtaaaac cagaaaacta
     5401 ccatttatta aactttcata atcttaacag gaccagatta tattttcatt gcagtggatt
     5461 agaagattat tggtcacaac aagcacccca aggcactacc aaattaatca ttcactacag
     5521 taatcaaatg caagagggtc gtgaaacaca tcagattcct tatcgaagtg ctatggttgt
     5581 gcattatgcc gctgaatggc taagcttcag actctttcat ctcatcaatc aattgcatta
     5641 aagcgtagca aagttcttgt gttcccttac tctctatagc ggaaatagaa aatactttgt
     5701 ctttccactc caagcctttt attatggatt gaattttttc ttctctttcc ttttcatcag
     5761 gtaacatatc gattttattt aaaaccaacc atctgggttt gttgagcaga tcgggattat
     5821 attgagtcaa ctcattgaga attgctttcg catcagcgac aggatcactc ccatccaaag
     5881 gcgcaatatc aataacatgc agcaagacac aggtacgtga aagatgtttt aaaaaccgat
     5941 gccccaatcc tgctccttga gccgcgcctt ctataagacc tgggatatcc gccatgacaa
     6001 aactcttata gggtgaaaca cggaccaccc ctaaacctgg atgtaaggtt gtaaatggat
     6061 agtcagccac tttggctttt gaactggaga cggctcttat taaagtagac tttcctgcat
     6121 tgggaagccc taacagacct acgtctgcca aaactcggag ctccaaacgc aaatttctgg
     6181 attcacccgg gcttccgggt gttgtttgtc taggtgatcg attcacgctg ctcttatagc
     6241 gagtgttacc caaaccatga aacccaccct gagcaatcag cacgggaata cctggttgac
     6301 tgatatcagc taacaattca cctgtatcag catcataaac catagtaccg actggcactt
     6361 tgatggttaa atcatctcct ttcttcccag tacaatttcc acccatccct gattgtccat
     6421 tctctgcttt gtattgacga gtataacgaa aatcaatcaa ggtatttaaa tcgctgctcg
     6481 cctcaaaata aatactgcct ccatcacccc catcaccccc gtcagggcca ccacgaggaa
     6541 taaatttttc tctcctgaag cttaagcaac cattccctcc ttttccggct tcaactttaa
     6601 taagtgcttc atcaacgaat ttcatgccta accttagact aaaataggac ttacgtaaaa
     6661 ttccaagctt aaggcaaaaa atattttcaa tgaatgcgct tagataagct aattgactga
     6721 attgaaaata ttttttagca aagagattgg gattttgcct aagtctgtaa aaaaaaaccc
     6781 cgtttccggg gttaaacaaa acgaattatg atgccattgg gtaatagcat tgtttcagct
     6841 taactcgcag cttcttctcg ttgctccggt aatatagtga catacttacg atttttttca
     6901 cccttggtag taaactgtac taacccctct accaacgcat acaaagtatg atctcgacca
     6961 caaccaacac caggtccagg atgaaaacgt gtgccacgct gccttacaat gatttcaccg
     7021 gcatttacaa attgaccacc aaaacgtttc acaccgaggt actttggatt cgagtcgcgg
     7081 ccattacgag tactaccacc tgctttctta tgagccattg ctatctcctc ttatttacca
     7141 atcgcagtaa ttttaacttg cgaataatat tgtctgtgcc ccatttgttt catatggtgc
     7201 ttacgtcgac gaaatttaat aattttaact tttttgtgac gaccatgatc aagcacttcg
     7261 gcctttacag ttgctttagc aacaaaaggt gtgccacaag tgaccttatc accatctact
     7321 agcattaata cttccgaaaa tttaatttca ttgccaacat tttcaggcag catttcaatt
     7381 tttaaaacgt caccttcttt cacggtatat tgcttaccgc cagttttaat taccgcatac
     7441 ataactattc tccaattcgc ctgattggct atcacaattc caacaaagtg ccatattcta
     7501 tgaaattatg actattactt caagttcatt ttaaaatatt gtatacttca ccacccttta
     7561 ccgattgact taatgttttt gaggctttcg agtaaatttt aatctggtcg cggattaatt
     7621 aggagctata gtcatattta agatagtgcc tagagggaac ccttaaattt ttggagtttt
     7681 tataatgtca actattcaat tagaagcaca atcaagaacg gatatgggga aaggtgcgag
     7741 ccgccgccta cgtcgtcttg aaaataaagt tcctgctgtc atatatggcg gaagcaaaaa
     7801 acctatggca atccatttta gccataataa agtgattaag gcattggaaa cagaaagcat
     7861 ttattccagc gtttttgaca tcacagtaga tggcaaagta gagcacgtta ttttgaaagc
     7921 cttgcaacgc catccctaca aaccaatcgt gttacatatg gatttacaac gcgtttccag
     7981 taaagacatt ctggttaaat tagtacctgt tcacttcata aatgaagaac aatctccagg
     8041 aattaaagct ggcggtattg ttcaacatac catgactcaa gttgaaatac gttgccaggc
     8101 aaaagatctg cctgaattta ttgaagtaga catgtctaag gtaggtatgg atgatgtggt
     8161 tcacttatcg gatttaaaac ttccaaaagg cgttcagctg acagtggatg tagccgatgg
     8221 tagtcatgat gctcctgtgg tgagtataca tgctgctaaa gtaagctcaa ctgaactgga
     8281 agaaacacct gaagttccag catcagctgt accgactaca gatcaagggg aaagtgctga
     8341 ataagcactt ccctgttata tatttatgca aatattcaga gcttaaccca gaatatttgc
     8401 ataaaatcta gtgctagaat agttaacaca aatccgctta attcaaattt tatagaaaat
     8461 ctatggtcat taaactaatt gtcggtttac gcaatcccgg atcggcttat gaacaaacca
     8521 gacacaacgc aggtgcgtgg ttagtgaccg ctttggccca aagacataat tcgcatttta
     8581 agattgacaa gaaaatgcaa gcggaattaa ctgaaattga tattaacaac cacccatgtc
     8641 gactagtgct tcctttgact tttatgaatc atagtggtca aacaacaaga ataatcagtc
     8701 aattctataa aattgaaccc ggtgaaatat taattgttca tgacgagttg gatttacctg
     8761 ttggacgaat caaattaaaa acaggtggtg gccatggtgg gcataatggt ttgagagaca
     8821 taactgctca attgggaaca ggtgaatttc atcgattacg tatagggata ggtcatcctg
     8881 gacataaaga tctagtccat caatacgtac tcagcaggcc ttctatgcac gacaggcaac
     8941 aaatttatga tgcaatagac agaggtatag cgataatacc tatagtattg tcaggggata
     9001 tggcccgagc aatgaatcaa gtaaacgctt aacataatga ggtatgactc atgggattta
     9061 aatgtggaat agtaggattg cccaatgtag ggaaatcgac tctttttaat gcattgacta
     9121 aggctgggat agaggcggcc aattacccat tttgtaccat tgagcccaat gtaggtatcg
     9181 tcaccgttcc tgattcaaga ctggataatt taagcgacat agtcaagcct cagcaggtgc
     9241 ttcatgccac catgcaattt gttgacattg caggattggt aaaaggcgct tccagtggag
     9301 aaggattagg aaatcaattt ttggccaaca taagagaaac agacgctatt gctcacgtcg
     9361 ttcgctgctt tgacaactcg gacgttgttc atgtggaggg gcgagttgac cccttaagtg
     9421 acatagaggt gattaataca gaactggcat tagcggatat ggagacattg gaaaagtcgc
     9481 tattaaaagt agggaaaaac agtaaaagtg gaaataaaga agccattttt gaattaaaaa
     9541 ctctggaaaa aattaaagca catcttgatg ccggatatcc tgtaagaacc cttgaactta
     9601 cccctgagga agaacaggta agcaaacgcc tttttctttt aacggccaaa ccagtacttt
     9661 acatagccaa tgttgacgat aacggctatg agaataaccc gctcctggat aaagttcagg
     9721 ctctggcttc ccaggaaaat gccagcatag tagccctttg tgcagcaaca gaagccgagc
     9781 tggttgagct ggatgaagaa gacagacaag agtttatggt tgatttgggg cttagtgaac
     9841 ctggattaaa taaagtgatc cgtgccgggt atgaattgct gggattgcaa acctatttca
     9901 ccgcaggagt gaaagaagta agagcctgga caatacgcaa aggagccaca gcaccgcaag
     9961 ctgccggggt cattcatact gactttgaga aagggttcat ccgcgcggaa gtgatttctt
    10021 acgatgattt tattcgcttt ggcggagaac aaggtgccaa agaagctgga aaattgcggc
    10081 ttgaaggcaa ggaatatatt gtctgtgatg gagacgtcat gcattttcgc tttaatgttt
    10141 aatcattagt attctgcaac tggtgatttt tcaagaattg agccaaagag cctcgagcct
    10201 ctatcgtttc atcattgcga gtggccaagt atcgagccag gcattgaagc aagtggaagt
    10261 atgctatcag gcgtatattt tcctactctt ataccacgct gactgttgtt tagcttgcga
    10321 tagctcaaac cgacgcagag tcaacagaag ataaacgatt tggtagaaaa tgaatagcaa
    10381 accaactccc tgtgcgtaaa tcaacctcaa tgaggacagt gtggctgttt tttaatctat
    10441 aattgaatta ataaaggaaa ttagccccat tgattaccgg ccaatgcgat tgaggtaggt
    10501 gaatgtcatt actaaagaaa acaagagagc aggattactc aatcctggtt accactcaag
//
LOCUS       DACTJE010000110        10525 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.110, whole genome shotgun sequence.
ACCESSION   DACTJE010000110 DACTJE010000000
VERSION     DACTJE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.110"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            154..942
                     /gene="truA"
                     /locus_tag="I9760_002106"
     CDS             154..942
                     /gene="truA"
                     /locus_tag="I9760_002106"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAT5043869.1"
                     /translation="MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISV
                     VCAGRTDTGVHATNQVIHFDCEKDRSIRAWIHGANTFLPKDICVKWGKEMPENFHARY
                     SAVSRRYRYVIYNGAIRPGLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVEC
                     QSNTPMRNIHQLQVTRHGDLVVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDV
                     LNAKDRKLGAETAPSYGLYLVQVTYPKEFGLLQNNPGPLFLWEK"
     gene            943..1566
                     /locus_tag="I9760_002107"
     CDS             943..1566
                     /locus_tag="I9760_002107"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HAT5043870.1"
                     /translation="MNPSRIRIKMCGMTRSEDIQYAIDLGVDAIGLIFYPKSVRNVSL
                     EKARIIVNNIPPFVDIVAVLVNPEQSFVQLIINEIPVQLLQFHGEESSEFCRQFNKPF
                     IKAIHPKTAIQIQSAVDEFFDASAILLDTPSDKERGGTGLTFDWNIIPENLSKPYILA
                     GGLNESNILEAITMCHPYAVDVCSGIEASPGVKDHLKMSRFIKAIWG"
     gene            1568..2767
                     /gene="trpB"
                     /locus_tag="I9760_002108"
     CDS             1568..2767
                     /gene="trpB"
                     /locus_tag="I9760_002108"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAT5043871.1"
                     /translation="MIKKELPDEFGHFGPYGGMFVADTLVHALKQLEHAYTKYRNDQD
                     FLSELHTELKDYVGRPNPLYHAVHLSKKIGGAQIYLKREDLNHTGAHKINNTIGQALL
                     AKRMGKTRVIAETGAGQHGVATATVAAKFGFQCVVYMGSEDIKRQSSNVYRMKLLGAE
                     VVPVTSGSKTLKDALNEALRDWVSHVDDTFYIIGTVAGPHPYPQMVRDFQAIIGVEAR
                     AQHMEKTGHLPDALVACVGGGSNAIGLFYPFLNDQSVMIYGVEAGGKGIETGEHSASL
                     IAGKPGVLHGNRTYLLCDEYGQVKDTHSVSAGLDYPGVGPEHAYLKDTGRVIYKAIND
                     SEAMDAFRLLTHTEGIIPALESSHAVAYAIQLAKTMSKEQSIIVNLSGRGDKDMHTVA
                     AIDGITI"
     gene            2793..3611
                     /locus_tag="I9760_002109"
     CDS             2793..3611
                     /locus_tag="I9760_002109"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAT5043872.1"
                     /translation="MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSG
                     ADVLELGIPFSDPMAEGPVIQRAMERALAHSIHCDDVLNMVRQFRKTDTETPVILMGY
                     LNPIEQYGYDLFAQQAVEAGVDGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSA
                     ERMNYINQHANGYLYYVSLKGVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPE
                     MAAQVAEFADGVIVGAALINEIIEAYEAKKDPLQASGALLSSMRQAIDNIGSMV"
     gene            3608..5263
                     /locus_tag="I9760_002110"
     CDS             3608..5263
                     /locus_tag="I9760_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HAT5043873.1"
                     /translation="MTEQTEKRAHFIRQLIIDDLANGKHQGIVTRFPPEPNGYLHVGH
                     AKSICLNFGLAEEFKGKCFLRFDDTNPIKEEEEYVNAIIDDVRWLGFEWCAMTHSSDY
                     YHELYELAIYLIKKDMAYVDSLSMEEIRAFRGTLQEPGRESPYRNRTIEENLDLFARM
                     KAGEFPDGTHVLRAKIDMKSGNLNMRDPVLYRIRHARHQRTGDEWCIYPMYDYAHPIS
                     DALEKITHSLCTLEFQDHRPLYDWLVDNLPLPAKPVQTEFARLNLSHTVTSKRKLREL
                     VEKKIVTGWDDPRLPTLRGMRKRGYPPAAIRQFCEIIGISRSDSVIDMSLLEECVRAE
                     FNKTAKRALCVMDPLKVVIVNYPENKVEQLQAAFYPQNPESASRNLPFSREIYIEKSD
                     FMENPPNKYFRLSPGAEVRLRHAYVIKCQEVIRNDQGEITELHCTYDENTLGKNPEDR
                     KVKGVIHWVSSSHAYPVTIYQYDRLFTDPNPAREEDYFQFLNHNSLQTLQGFCEPAMA
                     EQAEGDVFQFERLGYYCVNSVAEGCVQAFHRVVDLKDTWGKVS"
     gene            5273..6643
                     /locus_tag="I9760_002111"
     CDS             5273..6643
                     /locus_tag="I9760_002111"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAT5043874.1"
                     /translation="MLHLYNSLTRKKEPFVSLKPGTIGMYVCGITVYDHCHLGHARSM
                     VAFDVMVRYLRSQGFDVTYVRNITDIDDKIIARASERGVSIDELTAQYIDAMNNDTHA
                     LNILPPDHEPRATGHIETIIRLIQRLLEKGNAYVSENGDVCYEVDTFPEYGKLSHKDI
                     EGLVSGSRVEIVKEKRSPLDFVLWKKAKPGEPSWPSPWGEGRPGWHIECSAMAMHELG
                     EQFDIHGGGLDLQFPHHENEIAQSEAATGKPFANYWLHVGMLQVNGEKMAKSIGNFYT
                     IADVLKEHHPEVIRYFLLSSHYRSPLNYSEENLLNAKKALIRLYQAVKDVPPQTEDSK
                     LDEYWQVQFNQAMNDDFNTPVALSVLFQLAHEVNKSNSPALAHTLKNLAGILGFLQKD
                     PESFLQSGLAEEEKLVIEQLIAERLQARAERNWAKADQIRADLLSKGIELEDGATGTT
                     WRRIAE"
     gene            6815..8728
                     /locus_tag="I9760_002112"
     CDS             6815..8728
                     /locus_tag="I9760_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HAT5043875.1"
                     /translation="MNIKTYLRKNNLTLRFVGWFFLINSFIFWLVGLGYLKSILLNAS
                     LFKNYIADYSSLSGKILILVFSVLNYFTYMMFLAFVPAIPIIIMAFFLPYKRIIWIIS
                     VVAAATSLIFLFVDSQVFSMFKFHLNKTILSFIFNSDWSVIFTFSRYELILSFFMIVF
                     ILFLESIVAWLVWKKIVLKERLKIGKTISLFWLGGALISYFILLLSIAQNNNLFAQQI
                     PNLPLYNQLIAYTIPDKNADDILRRYSEHHFTQPLFPNYPLHYPLHPMQCKMPEKPYN
                     IILIMVDSLRYDSLRAKYMPNTAQFAKRSWQFSQHMSAGNATQPGLFSLFYSIPSNYS
                     TAVLEQKKSPVLIDLLLQHGFQTKIIWSGSMAPLPLDQTIYKKIANLNTNGAPIDDTG
                     DKDRYSTKEAIQFLTDPKTNNPFFLHIFYNAPHDYCRYQSFPQLYKPAIEECVRIGMT
                     NHVDPVPYYNRYLNAVTFIDQEISKVLGVIEKKGYLKNSIIIITSDHGQEFNDNRQNY
                     WGHTSNFTPIQLHVPLIIHWPGELARRFNYLTSSYDIVPTILQRLFACKNPVSDYSIG
                     YNLLIEGNRASFLLVGSYINMGIFEADRATALETSGRITITGMKAEPLDDAVPRVDVI
                     TQALDLMRKYYGK"
     gene            8858..9172
                     /locus_tag="I9760_002113"
     CDS             8858..9172
                     /locus_tag="I9760_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043876.1"
                     /translation="MANQTRENLINKAKNGSESIALIYSPIELHEYIVEIISKDSDHF
                     IHKGKEICHFNSIDEAVSRATELGAKEFFLCADNTYDECGSANSVQHFDYIPIYSKFK
                     RT"
     gene            9677..>10525
                     /locus_tag="I9760_002114"
     CDS             9677..>10525
                     /locus_tag="I9760_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043877.1"
                     /translation="MILEFFEVVLIMKSIEYFRNELNELFKSKKNGYKFKQEEKSPEQ
                     NTESDEYVLSFDTLPEEGIDRDNNTFYYTTSVDEICYEIKTSSGCLRTGSFHSNNHNN
                     LEERIFLNAAEKGFVENLEVLKNLATIERLIKELEKKGTEHEIKKYSSELNDEISLYN
                     DNLRKISNIFPDIDKKILKKLEINNSILPLPCPFDTRAVQVASHVPNQEGTYRRSQSS
                     NEYHHTNKRGNVITYKNFNELKNNDPNLGEIFLGTDNNSLYVKCKFKKSEPAKLLPIS
                     LYSETKN"
BASE COUNT         3333 a         1779 c         2132 g         3281 t
ORIGIN      
        1 taatcaacac cagtttggca tatttaggtt aaatgaaata gtatgagttc atgcaaaatg
       61 ataaaaccca tacaggcaaa gagttaatac tctaaaaatt atagacaaaa caaagagggt
      121 gttataaatt gaccttttta tcaggaaaat tgaatgcgta ttgccttagt ggttgaatac
      181 gatggtagcc aatatcacgg ctggcaggct caaacgggtt tgcatactat tcaacaagct
      241 gttgaatttg cattatcaaa agttgcagac agttctattt ctgttgtctg cgcaggtaga
      301 acagatactg gggtccatgc gaccaatcag gtgattcatt ttgattgtga gaaagatcgc
      361 agcatacgag cctggataca tggtgctaat acctttttac ctaaagacat ctgcgtcaaa
      421 tggggcaagg aaatgccaga aaattttcat gctcgttatt ctgctgtgag tcgacggtat
      481 cgctatgtca tttataatgg agctatacgg cctgggttat tacgcggtaa tgtgacatgg
      541 caatatcggc aactggatca tcgtttgatg caacagggtg gacaatgtct tctgggggaa
      601 aatgatttta cttcattccg ctccgtcgaa tgtcaatcta atacaccaat gcgtaatatt
      661 catcaattgc aagttacaag acatggtgat ttggttgttc ttgatattac agcgaatgcc
      721 tttttacatc atatggtaag aaatatagct ggtgtattga ttgctgttgg ctcgggaaaa
      781 caccctgttg gttgggttaa agatgtttta aatgcaaagg atagaaaatt aggcgctgaa
      841 actgcaccgt catatggcct atatttagtc caggtgactt atcccaaaga atttggattg
      901 ttgcaaaata atcctggccc tttgtttctt tgggagaaat aattgaatcc atctcgtata
      961 agaatcaaaa tgtgcggaat gacacgttca gaagatattc aatacgccat tgatttaggg
     1021 gtggacgcca ttggcttaat attttatccc aaaagtgtgc gtaatgtctc actggaaaaa
     1081 gccagaataa tagtcaataa tatacctcca tttgttgata ttgttgcagt tttggtaaac
     1141 ccagaacaat cttttgttca actgattatt aatgaaattc ctgtgcagtt attgcaattt
     1201 catggtgagg aatcttcaga gttttgcaga caatttaata agccgtttat caaagcaatt
     1261 catcctaaga cagcaatcca aatccaaagt gctgttgatg agttttttga tgcgagcgct
     1321 atattgttgg atacaccatc agacaaggaa cgaggaggaa cgggattaac ttttgactgg
     1381 aatataattc ctgaaaattt atccaagcct tacatattgg caggtggttt gaatgaatct
     1441 aacattttag aggcaattac catgtgtcat ccttatgcag tggatgtgtg cagtggtata
     1501 gaagcttcac ccggagtaaa agatcattta aaaatgagcc gatttataaa ggcaatatgg
     1561 ggatagtatg attaaaaaag agcttccaga tgagtttggc cattttggcc catatggcgg
     1621 tatgtttgta gccgatacat tagttcatgc tttaaaacaa ttagagcatg cctataccaa
     1681 atatcgtaac gatcaggatt ttctgtctga gttacacaca gaattaaaag attatgtggg
     1741 gcggcccaat cctctatacc atgccgtaca cttaagcaaa aagataggcg gtgcacaaat
     1801 ttatctcaag cgtgaagatt taaatcatac cggtgctcat aaaatcaata ataccattgg
     1861 acaagcctta ctcgccaaac gtatggggaa aactcgagtg atcgcggaaa caggcgcggg
     1921 tcaacatggc gttgcaactg ctacagttgc cgctaaattt gggtttcaat gcgttgtgta
     1981 catggggtca gaagacatta agcgtcaatc cagtaatgtt tatagaatga aattgttagg
     2041 tgctgaagtg gtgcctgtca cttcaggatc aaagacgttg aaagatgctt taaatgaagc
     2101 attgagagat tgggtgagtc atgtcgacga cacgttttat attattggta ctgttgcagg
     2161 tccccatcct tatcctcaaa tggtaagaga ttttcaagcg attattggtg ttgaagctcg
     2221 agctcaacat atggaaaaaa caggccattt gccagatgct ttggtagctt gtgtcggagg
     2281 tggatccaat gcaataggct tgttttatcc ctttttgaat gatcagtctg tcatgattta
     2341 tggggttgaa gcaggaggga aaggcataga aaccggtgag cattcggcat ctctgattgc
     2401 tggaaaacct ggtgtgttac acggtaatag aacctattta ttgtgtgatg agtatggaca
     2461 ggtgaaagac actcactcag tatcagccgg tctggattat cctggagttg ggcctgaaca
     2521 tgcttattta aaagatactg gacgggtcat ttataaagca ataaatgatt ccgaggccat
     2581 ggatgcgttt cgcctgttaa ctcatacgga aggaattatt ccagcattag aatctagcca
     2641 cgcggtagcc tatgccatac aattggctaa aaccatgtca aaagaacaaa gcatcatagt
     2701 caatctatct ggtcgtggtg ataaagatat gcatacagta gctgctattg acggtataac
     2761 aatttaattt tgataaatta ttcaggtaca acatgaaccg tattgataag actctggaaa
     2821 aattaaaagc caatagaaaa aaaatgttaa gtccttatat tactgcaggc gatccatatc
     2881 ctgagttaac agtaagttta atgcatcaat tggttaaatc aggggcagat gtattggagc
     2941 tgggaatacc cttttctgac cctatggcag aaggtcctgt tatccaaaga gcaatggaac
     3001 gagctttggc gcattcaatt cattgtgatg acgtattgaa tatggttagg caatttcgca
     3061 aaacggatac cgaaacgcct gtgattctta tgggatattt aaatcctata gaacaatatg
     3121 gctatgatct ttttgcccag caggcagtgg aggctggggt tgatggaaca atattagttg
     3181 atttgcctcc tgaggaagct gatggagtat cgcgagtttg gcaaaaacat ggtttgtaca
     3241 gtatttattt atgttcgcca accacctcag ctgaaagaat gaactatata aatcagcatg
     3301 ccaatggtta tctgtattat gtttcattga agggagtgac aggttctgac gcgcttaagt
     3361 tgccagaatt aaaagctcaa tatctacaac gaaaggcaca atcaaaatta ccgcttatgg
     3421 ttgggtttgg aataaaaact ccggaaatgg ctgcacaagt tgctgaattt gctgatgggg
     3481 tcattgttgg ggcggcactg ataaatgaaa tcattgaagc ctatgaggcc aagaaagatc
     3541 cgctgcaagc aagcggggct ctattgagtt ccatgaggca agctatcgat aatattggaa
     3601 gtatggtatg acagaacaaa ctgaaaaaag agctcacttt attagacaac tcattataga
     3661 tgatttggca aatggaaaac atcagggaat cgttactcgt ttccctcctg aaccaaatgg
     3721 atatctccat gttggacatg ccaaatctat ttgtcttaat tttggtttgg cagaagagtt
     3781 taagggaaaa tgctttttac gttttgatga caccaatcca attaaagaag aagaagaata
     3841 tgtaaacgcg atcattgatg atgttcgttg gttgggattt gaatggtgtg ccatgacgca
     3901 ttcttcggat tactatcatg agctgtatga attagccatt tatcttatta agaaagatat
     3961 ggcttatgtc gatagcttga gtatggaaga aattcgtgct tttcgcggca ctctacaaga
     4021 accagggcgc gaaagccctt atagaaacag aacaattgaa gagaatctgg atttgttcgc
     4081 tcggatgaaa gctggtgagt ttcctgatgg aactcatgtt ttgagagcaa aaatagatat
     4141 gaaatcaggt aatttaaata tgcgtgatcc ggttttatat cgtatacgcc acgcccggca
     4201 ccagcgtaca ggagatgaat ggtgtattta tcccatgtat gattatgctc atcctatttc
     4261 tgatgccctg gaaaaaataa cacattcttt atgtacgttg gaattccagg atcatagacc
     4321 tttgtatgat tggctggttg ataatttacc tttgcctgcc aaaccagtac aaaccgaatt
     4381 tgccagatta aatttatccc atactgttac gagcaagaga aaattacgtg aattggttga
     4441 aaaaaaaatc gttacaggat gggatgaccc acgattgccc acattacgcg gtatgcgcaa
     4501 aaggggatac ccacctgctg caattcggca attctgtgaa ataattggca tatcaagaag
     4561 tgattcagtg attgatatga gtttacttga ggaatgtgta cgcgctgaat ttaataaaac
     4621 tgcaaaacgg gctctctgtg ttatggatcc cttaaaagtt gttattgtaa attatccgga
     4681 aaataaagta gaacagcttc aagcagcttt ctaccctcaa aatcctgagt cagcgagtag
     4741 gaatttaccc ttttcccgtg aaatttatat tgaaaagtct gattttatgg aaaatccgcc
     4801 taataaatat ttccggttat ctcctggagc tgaagtacgc ttacgtcatg cctatgttat
     4861 aaagtgccag gaagttatcc gtaatgacca aggcgaaata acagaattgc attgcactta
     4921 tgatgaaaat actcttggca aaaatccgga ggatcgcaag gtgaagggtg tcattcattg
     4981 ggtgtcttca tcacatgcct atcctgtcac tatttatcaa tacgacaggt tatttacaga
     5041 tcctaatcct gcacgggaag aggattattt tcagtttctg aaccataact ctttacaaac
     5101 cctccaggga ttttgtgaac cggcaatggc tgagcaggca gaaggagatg tgtttcaatt
     5161 tgagcgttta ggttattatt gcgtcaattc agtggctgag ggctgtgttc aagcctttca
     5221 tcgggtagtg gatttgaagg acacttgggg taaagtgagt tgagaggcag acatgttaca
     5281 tttatataat tctttgacca gaaaaaaaga accatttgtt tccttaaagc ccggaacgat
     5341 aggtatgtat gtatgcggaa ttactgtgta tgaccactgc cacttagggc atgcccgttc
     5401 aatggttgcc tttgatgtaa tggttcgtta tctgcgctca caaggattcg atgtaactta
     5461 tgtacgcaat ataactgaca tagatgataa aatcatcgca agagcctcgg agagaggtgt
     5521 atctattgat gaattgaccg cacaatatat tgatgcaatg aataatgata ctcatgcatt
     5581 aaatattttg ccacctgacc atgagccaag ggcaaccggg cacattgaaa ctatcattcg
     5641 cttgattcaa cgtttgcttg aaaaaggtaa cgcgtatgtc agtgagaatg gcgatgtgtg
     5701 ctatgaagta gatacctttc ccgaatatgg gaaattatcg cataaagata ttgaaggctt
     5761 ggtttcgggt tctcgcgtag agattgtaaa agagaaacgt tcaccgcttg attttgtgct
     5821 atggaaaaaa gcaaaaccgg gtgaacccag ttggccttca ccgtgggggg aagggcggcc
     5881 tggatggcac attgagtgtt cagctatggc tatgcatgaa ttaggtgaac aatttgatat
     5941 tcatggaggt ggattggatt tgcaattccc acatcatgaa aatgaaattg ctcaaagtga
     6001 agcggctacg ggtaaacctt ttgctaatta ttggctgcat gtcggaatgt tgcaggttaa
     6061 tggcgaaaaa atggcaaaat caatagggaa cttttacact attgccgatg ttttaaaaga
     6121 gcatcatccg gaagtcatcc gctattttct attaagtagt cattatcgca gtcctttgaa
     6181 ttattcagaa gaaaatttgt taaatgccaa aaaagccctt atcaggcttt atcaagcggt
     6241 taaagatgtt ccgccacaaa ccgaagatag taaactggat gagtattggc aagtgcaatt
     6301 taatcaggca atgaatgatg atttcaatac tcctgttgca ttatcggttc ttttccaatt
     6361 ggctcatgag gtaaacaaaa gtaattcacc tgcattggct cataccctga aaaaccttgc
     6421 tggtattctt ggatttttac aaaaagatcc cgaatcattc ttgcaatccg gtttggcaga
     6481 ggaagaaaaa ttggttatag aacaactcat tgccgagcgt cttcaggcgc gagcggaacg
     6541 caattgggca aaggcagatc agattagagc tgatttattg agcaaaggca tagagctgga
     6601 agatggtgca acaggaacaa catggcggag gattgctgaa taattgttcc ttctatgcgc
     6661 ttctcttgaa tcagaatgga cttctatctc atacttaata tagactgcga ttaccttttc
     6721 agaaatttta aatcaggtat attggctaca ttaattgctg tttattgact ggattgttca
     6781 ttaagataaa ttcgaaatta catttgaatt ggtaatgaat ataaaaacat atctaagaaa
     6841 aaataattta accttacgat ttgttggatg gttcttttta ataaactcat tcatattttg
     6901 gcttgttggc ttggggtatt taaagtccat tttattaaat gcctccctgt ttaaaaatta
     6961 tatcgctgat tattcaagcc tatcaggaaa aattctcatt ctggttttct cagttttaaa
     7021 ttattttact tatatgatgt ttttagcttt tgtccctgca ataccaatca taataatggc
     7081 atttttttta ccttataaac gaataatttg gattatcagt gttgttgcgg ctgccacaag
     7141 tcttattttt ttatttgttg atagtcaagt tttttcaatg tttaagtttc atcttaataa
     7201 aacaatatta tcgtttattt ttaattcaga ttggagtgtc attttcactt tttccagata
     7261 tgaactgatt ttgtcttttt ttatgattgt ctttatactc tttctggagt ctattgttgc
     7321 ctggttggta tggaaaaaaa tagttttaaa agaacgtttg aaaattggta aaactatttc
     7381 attattttgg cttggtgggg cattaataag ttattttata ttgctcttat cgatagctca
     7441 aaataataat ttatttgctc agcaaatacc taatttgccc ctttataatc aactcattgc
     7501 ttatactatc cctgataaaa acgcggatga tatattacgc cgctacagtg agcaccattt
     7561 tacacagcct cttttcccca attatccatt gcattatccc ttacatccca tgcaatgtaa
     7621 gatgcctgaa aaaccttata acattatcct tataatggta gattcacttc gctatgattc
     7681 cctaagggca aaatatatgc cgaatacggc acaatttgca aaaagaagtt ggcaattttc
     7741 tcagcatatg agtgctggaa atgccacaca accaggattg ttttctttat tttattcgat
     7801 tcccagtaat tactcgacgg cagtcctgga gcaaaaaaaa tcaccagttt taattgattt
     7861 attattacaa catggttttc agacaaaaat tatttggtct ggctctatgg ctccactgcc
     7921 attagatcag actatttata aaaaaattgc caatctcaat accaatggag cgccaataga
     7981 cgataccgga gacaaggaca gatattccac caaggaagct atccaatttt taacagatcc
     8041 taaaacaaat aacccattct ttcttcacat tttttacaat gcgccacatg actattgtag
     8101 atatcaaagt tttccgcagt tatacaaacc cgcaatagaa gaatgtgtac gaattggaat
     8161 gacaaatcat gttgatccag taccttacta caatcgttat ttgaatgcag tcacatttat
     8221 tgaccaggaa atttcgaaag tattaggggt tatcgaaaaa aaaggttatt taaaaaacag
     8281 catcattata attacctctg atcatggcca agaatttaat gataatcgtc aaaattattg
     8341 gggacatacc agtaatttta cccctattca actacacgtt cccttgatta ttcattggcc
     8401 aggggagtta gctcgtcgtt ttaattatct caccagcagc tatgatattg tacctacaat
     8461 attacagcgt ttatttgctt gcaaaaatcc tgtttctgac tacagcatcg ggtataactt
     8521 gctaattgag ggcaatcgag cctcatttct attggtagga agttatatta atatgggaat
     8581 ttttgaagca gatcgagcca cagcattaga aacctccgga agaattacaa ttactggtat
     8641 gaaggcagag cctcttgacg atgcggtacc cagagtggat gttattaccc aggcattgga
     8701 tttgatgcgc aagtattatg ggaaataaaa gattgtcttt aaaaataaat tggcaattaa
     8761 cctttttatt ctcaatttag gccatacagc ggatatcttt aaatttctag gcaaaaatat
     8821 ggctataatt atatataatt tgcttgggaa ttaagtaatg gcaaaccaaa cacgtgaaaa
     8881 tttaattaat aaggcaaaga acgggtccga atctatcgct ttaatttatt ctccaataga
     8941 acttcatgaa tatattgttg aaattatttc taaagattca gatcacttta tccataaagg
     9001 aaaggaaata tgtcatttta attcgattga tgaagcggtg tctcgtgcta ccgaattagg
     9061 ggcaaaagag ttttttcttt gtgcagataa tacatatgat gaatgtggtt ctgcaaattc
     9121 tgtgcaacac tttgattata tacctattta ttctaaattt aagcgcactt gagtttggcg
     9181 catcaatctt ggttgcgagc tttttgcatt tctttttcgg ttcaaactct ataagccgac
     9241 gaatgacact ggcttttcct actctaaatt ttgtagccac ctcttggatg gtcaattttt
     9301 tttctgctct tatttttaat acatgtttgc aaaaaaacaa ggaattcata attaattatt
     9361 attaataatc aattggttat atgttggtta taaaacatta ggcaaacgct caatgaacct
     9421 caaatactat gttatctctc aatacataaa aacatgctct ggctaactta ttagccactg
     9481 ttttaatctg agattcctta tacctcgatt caaatggtga gtggctcagc gtacatttag
     9541 agcaaccgct ttatatcaaa aagcctacct ttcatagtct tttgtcgcat ttacgacgat
     9601 tttcatcatt ttactttaat cattggttca taaaataaaa accaaatgat cataaagtga
     9661 tcaatttaga taagaattga tattggaatt ttttgaggtt gttttgatta tgaaatctat
     9721 tgaatatttt agaaatgaat taaatgaatt atttaagagc aaaaaaaatg gttataaatt
     9781 taaacaagaa gaaaaatctc cggaacaaaa tacagagtct gatgagtatg tattgtcttt
     9841 tgacacgctt cctgaggaag ggatagatag agataataac actttttatt atacgacctc
     9901 tgtagatgaa atatgttatg aaattaaaac atcctcagga tgtttacgta cgggaagctt
     9961 tcactccaat aatcacaata atcttgagga gcggattttt cttaatgcag ctgaaaaagg
    10021 gtttgtcgaa aatttagaag tcttaaaaaa tctagcaacg atagaaagac tcataaaaga
    10081 attagaaaaa aaagggaccg aacatgaaat taagaaatat agctctgagt taaatgatga
    10141 aattagttta tataatgata acttacgaaa aataagcaac atattccctg atattgataa
    10201 gaaaattcta aaaaaattag aaataaacaa tagcattctg cctttgcctt gtccatttga
    10261 tacacgagca gtacaagttg ctagtcatgt tccaaatcaa gaaggcactt atcgtcgttc
    10321 tcagagctct aacgagtatc atcatactaa taagagaggt aatgtaataa cttataaaaa
    10381 ttttaatgag ttgaaaaata atgaccccaa tttaggggag atttttttag gaactgataa
    10441 taattcatta tatgtgaagt gtaaatttaa aaagtcagaa cctgctaaat tattacctat
    10501 cagtttatat tcagagacaa agaat
//
LOCUS       DACTJE010000111        10358 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.111, whole genome shotgun sequence.
ACCESSION   DACTJE010000111 DACTJE010000000
VERSION     DACTJE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.111"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            36..749
                     /locus_tag="I9760_002115"
     CDS             36..749
                     /locus_tag="I9760_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043878.1"
                     /translation="MNSQSDFNLPILSSWYKKKFLGYASQVLEAQQKMTSSKGKNILH
                     YTLRKKRKHERMSHYPKGDRIDRSTGSQYFYHCHRENFESNEHGHFHCFLRYKHIPKR
                     IKPAPLEDWDKYIDNPMTHLVAIGMNQFGQPIRLFTVNRWVTSEIWYGAEHIPYFLKS
                     YKMTLIDDPYWQVLDQWVEGMLHLFAPQIAWLHQERDKRIQLHQLNSPNDNPYTNHEL
                     EELSEINIDLKKQIEWVIS"
     gene            902..3244
                     /gene="purL"
                     /locus_tag="I9760_002116"
     CDS             902..3244
                     /gene="purL"
                     /locus_tag="I9760_002116"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAT5043879.1"
                     /translation="MQNLTETLDFSSLNRGEIEKLLRDYNLNLSVDEALTIQNEFLKR
                     PPTISECVLWSIQGSEHCSYKSSRIHLKQFNTSGPHVILGAKEDAGIVSVAQDKNGYR
                     YGVIVSHESHNHPSQIVPYEGAATGVGGNVRDVCCMGGEVIAVADSLRFGDIKRARTH
                     WIQEGVVSGIAGYGNPLGIPNIAGDVYYDPAYNENCLVTVVTLGIVREDHIIHSYAPP
                     EAENYVFILVGKPTDNSGFGGASFASTVLEEDSQEKNKGAVQEPNAFLQRHLLKANYS
                     LFQLLREKNLIDKVGFKDLGAGGVACASIELAEAGGSYGAEIDLDKVPTGMPGLMPSV
                     ILCSETQERFMWVVPPDLVDTILKHYNETFALPQVSEGACAAVIGKIRTDGLYVVNYK
                     GRELVRAKVPDVTKGIVYNRPHASSQKQNTEPVFLPPDDYNQILLQLLAHENIASKEP
                     IFEMYDKQVQGRTLIQAGWADAGVLQPFNESKYPEEIRKTGIALSLDQNPRYNKIDAY
                     WGAVNAVVESVRNITAVGATPVAITDCLCFGNPEKPEQMREFVDSVRGISDACAAVHL
                     KDHPQSTLPVIAGNVSLYNESVKGAIPPSPMISCLGTLPDIDFAITFDFKKSDSLLIL
                     IGERKDECGGSVYYQLHNELGSNVPKPDLSLFNREIHAVSSAIQHGLVNAAHDVSEGG
                     VAVALAEMSFKNSLGVAVQINGELSADKLLFGETGGFILEIDKQHKAAFDKLVTQYQV
                     PYMVIGHTTEQPVLQMNSVINLPVEEARQAWENGLRERLL"
     gene            3244..4287
                     /locus_tag="I9760_002117"
     CDS             3244..4287
                     /locus_tag="I9760_002117"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAT5043880.1"
                     /translation="MSTINYKSAGVDIEAGNEAVARIKRNVEKTFSPQVLTGIGSFGA
                     MYDLKPFMEKFKHPVMVQSIDGVGTKTIVARMMQKFDTIGIDLVSATTNDIIVLGARP
                     LTLLDYIATDRLKPEYIEEIIIGMTQACLENNISLVGGETAEMPDTYLKGEHDLVGII
                     TGVVEKDEAILGKDITPGDSVLAFPSSGLHTNGYSLARKLLFELGGYTVESQLDELDA
                     TVGEALLVPHINYTNPVLHLLNNKIPIKGMAHITGGGVLENIPRVLPNHCSVEINKNS
                     CPVLPIFNVLKALGKLDDQEMYRTFNMGIGLVMIASPGVVSEIYTALKEFPNFPVYEV
                     GKVVSGKPEVRLL"
     gene            4287..5546
                     /gene="purQ"
                     /locus_tag="I9760_002118"
     CDS             4287..5546
                     /gene="purQ"
                     /locus_tag="I9760_002118"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HAT5043881.1"
                     /translation="MKIAVVQFPGSNCERETMLAVQRAGMTPVEFLWNDSSQKLREMD
                     GYIIIGGFSYEDRSRAGIIAALDPVMKEIKLQSELGKPVLGICNGAQILVETGLVPGL
                     KNYQIGMALTENKRIQDGKILGTGFYNSWIHMRTGDRVRQNAFTRYLSKDSVLSIPAA
                     HAEGRFVMPVKLLQEIEEEGLNAFQYCDAQGNRDDNFPVNPNGSLRNIAAIINKAGNV
                     MAIMPHPERTFAGDPIFHSMKEYIKEKKTFKQQTLSYQPEPVKIIPYDKKDNAHELIV
                     KLIITDNAALTVQNTLRQLGIPVTVNRMMYWHVDTNFPETIEKIKQTGVLYNDRKEYL
                     VTSEHIFSSAAKAFLVKSKDDMIGQQKLQMLRDHFSIDGVDAIHHGVLWMFSSENVKI
                     QELAESILATNIIFNPNAHICYEYQCP"
     gene            5527..6507
                     /locus_tag="I9760_002119"
     CDS             5527..6507
                     /locus_tag="I9760_002119"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAT5043882.1"
                     /translation="MNTNVPSQEDLLAALPFCLTETSLPFGKKYKGKVRDTYDLGDQL
                     ILVTTDRQSAFDRCLAAVPYKGQVLNLTSAWWFKNTQSIVPNHLIAVPDPNVAIAKKC
                     KIFPIEFVVRGYISGSTSTSLWTQYQKGVREYCGITFPDGLRKNQKLESPVITPTTKE
                     TLHDRPISPHEIVAEGWMTQEDWDETSSYALKLFQHGMEVAQQHGLILVDTKYEFGRD
                     AEGRIVLVDEIHTPDSSRYWLFNGYQERFDAGKEPENIDKEFLRLWFVDHCDPYKDEV
                     LPQAPQELIVTLASRYIQLYEMITGESFVYDSNPGPVNDRILHNIQRWLG"
     gene            6530..8029
                     /gene="purF"
                     /locus_tag="I9760_002120"
     CDS             6530..8029
                     /gene="purF"
                     /locus_tag="I9760_002120"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAT5043883.1"
                     /translation="MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFK
                     HGLGLVRESIFSEDILKLKGNIGIAHVRYPTAGGYSEADVQPLWIGSPRGIALAHNGN
                     LVNYGELVDDLCGKQHRHLNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVN
                     EIHQRVQGSYSVVSLVIGKGLVAFRDPHGIRPLVWGERVHENGSKDYIFASETTPFYA
                     LGFEPKGDLQPGEVAYVNQQGQLYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRA
                     RLRMGQNLAKSWKKRFPDTLPDVVIPAPSTANTAALAFANELGIRYSEGLYKNPFIGR
                     TFIMPNQESRSRQVRYKLTPQKTEINKKKVLIVDDSIVRGTTSREIVRMIREYGASEI
                     YFVSTCPPIKNPCFYGIDIPSRKKLIAAHKTEEEIRQFLGVDKLLYQTQENLIEAVSR
                     RGQYDMSKPCMACMDGKYICGQITEEKIKLLEKKRLTENDQETEIGEMS"
     gene            8026..9345
                     /gene="purD"
                     /locus_tag="I9760_002121"
     CDS             8026..9345
                     /gene="purD"
                     /locus_tag="I9760_002121"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAT5043884.1"
                     /translation="MINILIVGSGAREHAIATALSKSENKPKIYCFGTTRNPGIQRLT
                     SDYWVGDITDPEQVNQQAKEWEINLAIIGPEAPLEHAVADALWDCGIPVIGPRKNLAK
                     IETSKAFARELMEKYGIPGLPKYKVFTSLEGIGEFLSELGENNYVVKANGLMAGKGVK
                     VGGEHLLSFEEALNYCKEIFTHNQSLVIEEKLIGQEFSFMCFSDGISLVPMPIVQDHK
                     RAYEHDEGPNTGGMGSYSAADHSLPFLSPEDVRAAFEINQSVIKALMTEMSDKYIGIL
                     YGGFMATAHGVYVIEFNARFGDPEALNVLSLLESDFVKLCVAMANGNLQKESVRFLNQ
                     ATVCKYAVPDGYPDNPLRHVEIDISAVEPKDTLYLAAVNFKNGKLYATGSRTAAYVGI
                     ANTISEAEEIAEQQIKNIKGPLFHRKDIGTSPLINTRVEVMRRLRAL"
     gene            9342..9920
                     /locus_tag="I9760_002122"
     CDS             9342..9920
                     /locus_tag="I9760_002122"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAT5043885.1"
                     /translation="MIRLGILGSTRGTNMLALVDAINEGTLKAKIELVISNKPDAIIL
                     ERAKSLGLNAQFVNPEGLNRIDFDKKVSDILINHQIDLIVLIGYMRILSADFVNKWNN
                     QVINVHPSLLPAFAGKMDMDVHQAVLDSGLKETGCTIHFVTEEVDAGPVILQKKCPVL
                     EGDTAQTLKARVQQLEGIALVDAINLIASKGL"
     gene            complement(9917..>10358)
                     /locus_tag="I9760_002123"
     CDS             complement(9917..>10358)
                     /locus_tag="I9760_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAT5043886.1"
                     /translation="LPASLPGFSSELVRLPEKPHLIIIVRKNGNELNAPIDVVVVNLE
                     QEKRQTLTKEDFQLIAKNYCNQYFSKNRPSVCDSLREKLYSHVLKSAVNNRVHTDTSI
                     KLNGIHLFEFKETSLNTFFTIEKEKNYSHGNEDNTALTGNTISH"
BASE COUNT         3173 a         1765 c         2299 g         3121 t
ORIGIN      
        1 acctcttttt attacggttt attttttaag taattatgaa tagtcaatca gattttaact
       61 tacccatatt gtccagctgg tataaaaaga aatttcttgg ttatgcttca caagttcttg
      121 aagcccaaca aaaaatgaca tccagtaaag gtaaaaatat tctgcactac acgcttagaa
      181 aaaagcgaaa gcatgaacgc atgagtcact atcctaaggg agacaggatt gatcgtagta
      241 ctggctcaca atatttttat cattgccata gagaaaattt cgaaagtaat gaacatggtc
      301 attttcattg ttttttgcga tataaacata taccgaagcg tattaaacca gctcctttag
      361 aggattggga taaatacata gataatccca tgactcacct tgttgcgata ggtatgaatc
      421 agtttgggca accgatacga ttatttacag tcaatcgttg ggtaacatca gaaatttggt
      481 atggtgctga gcatattcca tatttcctga aatcttacaa gatgacatta atagacgatc
      541 cttattggca agtattagat cagtgggtgg aaggcatgct ccatttgttt gctccccaaa
      601 ttgcctggct tcatcaggag cgagataaac gcattcagct tcaccaactg aatagcccaa
      661 atgataaccc gtacactaac catgagctgg aagagctttc tgagataaat attgatttga
      721 aaaagcagat tgagtgggta ataagttaaa ttccaaaatt agaaccacaa cttaatttat
      781 cttaattatt ggtttttcat tgcttatacc ccgtgtaaaa atatacaata tctactgctt
      841 tggggagaga ttagtccttt actgatcaat cagagattaa ttctatctgg tatagagatt
      901 catgcaaaat cttactgaaa cccttgattt ttcttctttg aatcgtggag aaattgaaaa
      961 attactgcgc gattataatt taaatctttc tgtagatgag gcgttgacta tacagaatga
     1021 attcctcaaa agacccccga ctatttcaga gtgtgtactt tggtctattc aaggatcaga
     1081 acattgttct tataaaagca gccgtattca tttaaagcaa ttcaatacct caggccctca
     1141 cgtgatactg ggcgccaagg aggatgcagg tatagtatct gtagcccaag ataaaaatgg
     1201 ttatagatat ggcgttattg tcagtcatga atcacataat cacccatctc aaattgttcc
     1261 ctatgaaggc gcagctaccg gagttggtgg taatgtccgt gatgtctgtt gtatgggtgg
     1321 ggaagttata gcagtagcag acagtttgcg tttcggagat atcaaacgtg cacgtactca
     1381 ctggatacag gaaggggtgg tttcaggtat tgccgggtat ggcaatcctt tgggaatacc
     1441 gaatatagct ggtgatgtgt actatgatcc ggcctacaat gaaaattgtt tggttactgt
     1501 agtcactctg gggatagtaa gagaggatca tattatacat tcttatgctc cgcccgaagc
     1561 tgagaactat gtttttattt tagtaggtaa gccaactgat aatagtgggt ttggcggcgc
     1621 gagctttgcc tcaacagtat tggaagaaga ttcacaagaa aagaataagg gagctgttca
     1681 agagcccaat gcttttttac agagacatct ccttaaagct aattattcat tatttcaatt
     1741 actaagagaa aagaatttaa ttgacaaggt aggctttaaa gatttaggag caggtggcgt
     1801 ggcctgcgca agtatcgagt tggctgaagc cggcggttct tatggcgccg aaattgatct
     1861 ggacaaggta cccacaggaa tgccaggatt aatgccttca gtcattctat gctctgaaac
     1921 tcaggagcgt tttatgtggg ttgttccccc tgatttagta gatactatcc tgaaacacta
     1981 taatgaaacc ttcgcgttac cacaggtttc tgaaggggca tgcgctgcag ttatcggcaa
     2041 aattcgtacc gatggattat acgtggtgaa ttacaaaggg cgagaacttg tgagagctaa
     2101 agtccctgat gtcacgaaag gcattgttta taacaggcca catgcctctt cgcagaagca
     2161 aaacacagaa ccagtttttc tcccaccaga tgattataat caaattttgt tgcagttatt
     2221 ggcccatgag aatatagcga gcaaggaacc tatttttgaa atgtatgaca agcaagtgca
     2281 gggtcgcacc ctcatacaag caggatgggc tgatgctgga gtattacaac catttaatga
     2341 atcgaagtat cctgaggaaa ttcgtaaaac aggtattgcc ttgtctttag atcaaaatcc
     2401 ccgctataac aaaattgatg cctattgggg agctgttaac gcggttgtcg aatcggtacg
     2461 gaacatcact gcagttggag ctactccagt cgcaataaca gattgcttgt gttttggtaa
     2521 tccggaaaag ccagaacaaa tgcgcgaatt tgttgattct gtgcgaggaa ttagtgatgc
     2581 ctgtgccgct gttcatctga aagatcatcc ccaatcaact ttgcctgtca ttgccgggaa
     2641 tgtctcattg tataatgaat cagtgaaagg ggctattcct cccagcccca tgattagttg
     2701 cctgggaacg cttccagata ttgattttgc tattaccttt gattttaaga aaagtgatag
     2761 cctattgata ttgattggag agcgtaaaga cgaatgtggt ggtagcgttt attatcaact
     2821 ccataatgaa ttgggttcta atgttccaaa accggattta agcttgttta atagagaaat
     2881 ccatgctgtt tcatcggcaa ttcagcatgg gttggttaac gctgctcatg atgtctcaga
     2941 gggaggggtt gctgttgctc ttgctgagat gagttttaag aactccctgg gagttgctgt
     3001 acagattaat ggagagttat ctgcggacaa acttttattt ggagagacag gcggttttat
     3061 tcttgaaatc gataaacaac ataaagccgc ttttgacaaa ctggttactc aatatcaagt
     3121 cccttacatg gttatcggac atactacaga gcagccagtt ttacagatga attcggttat
     3181 caacttgcct gtcgaagaag cgaggcaggc ttgggaaaat ggcttaagag agaggctatt
     3241 ataatgtcta ctatcaatta caaatcagcg ggtgtcgata tcgaggcggg aaatgaagct
     3301 gtcgcacgca ttaaacgtaa tgttgaaaaa acattttcgc ctcaagtcct gacaggaatc
     3361 ggtagttttg gcgcaatgta tgatttgaag ccttttatgg aaaaattcaa acaccccgtc
     3421 atggttcaaa gcattgatgg cgtaggcacg aagacaatag ttgctcggat gatgcaaaaa
     3481 tttgatacaa tcggaattga tttggtcagt gcgaccacca acgatattat tgtgttgggt
     3541 gcaaggccat taacactttt ggactatata gcaactgatc gattgaaacc tgaatatatt
     3601 gaagaaatta ttattggcat gacgcaagct tgcctcgaaa ataatatttc cctggtcggt
     3661 ggtgagacgg ctgaaatgcc tgatacctat ctgaagggag agcatgattt ggtaggtatt
     3721 atcactggtg tagtggaaaa ggatgaagcc atactaggaa aagatataac tccaggcgat
     3781 tcagtcttgg catttccttc cagtggctta cataccaatg gatattcact tgccagaaaa
     3841 cttttgtttg aactaggtgg atatactgta gaatcccaac ttgacgagtt ggatgctaca
     3901 gttggtgagg ctttactggt acctcatatc aattatacaa atcctgttct acacctgttg
     3961 aataacaaaa tccctatcaa gggaatggct catattactg gcggaggtgt actggaaaac
     4021 attccgcgag tattacctaa tcattgttcc gttgaaatca ataagaattc atgtccggtg
     4081 ttgccgatct ttaatgtgct aaaagcattg gggaaactgg atgatcagga aatgtatcgt
     4141 acttttaata tgggaatagg attagtaatg atagcttccc ctggtgttgt ttctgaaatt
     4201 tatacagcgc taaaagaatt ccctaatttc cctgtttatg aagtaggaaa agtcgtatca
     4261 ggaaaaccag aagtgagatt attgtgatga agattgcagt tgttcagttt ccaggctcca
     4321 attgcgaacg tgagacaatg cttgcggtgc aacgagcagg tatgactcca gttgagtttc
     4381 tgtggaatga ttcatcacaa aaactgcgag aaatggatgg ttatattatt attggtggtt
     4441 tttcatatga ggatagatca agagcaggaa ttattgcagc ccttgatccg gtaatgaaag
     4501 aaataaaatt gcagagtgaa ttgggaaaac ctgttcttgg tatttgcaat ggagcacaga
     4561 tccttgttga aacaggcctc gttcctggtt taaaaaacta tcaaattggt atggctctga
     4621 ctgagaataa gcgaattcag gatggaaaaa ttttaggaac aggattttat aacagctgga
     4681 ttcatatgcg aaccggtgac cgagtgaggc aaaatgcatt cacccgctat ttatcaaagg
     4741 attctgtatt atcaattcct gctgcccatg ctgaagggcg ttttgtgatg cctgttaaat
     4801 tattgcaaga aattgaagaa gaaggtttaa atgcgtttca atattgtgat gcgcagggaa
     4861 acagagatga taatttcccc gttaatccta atggttctct acggaatatt gccgctatta
     4921 ttaataaagc gggtaatgtc atggccataa tgcctcaccc tgaacgtaca tttgcgggag
     4981 atcctatatt ccattcaatg aaagaatata ttaaggagaa aaaaacattc aagcagcaaa
     5041 cattatctta tcaaccggag ccagttaaaa taattcctta tgacaaaaaa gacaatgccc
     5101 atgagttgat tgttaaattg attattaccg ataatgctgc tttaacagta caaaatacgc
     5161 taagacagtt aggtatacca gtcacagtta atcgtatgat gtattggcat gtcgatacaa
     5221 attttcctga aacaatcgaa aaaataaagc agacaggggt tttatataat gatcggaaag
     5281 agtatttggt aacgtctgag catatatttt catcagcagc caaggctttt ttggttaaat
     5341 caaaagacga tatgatcggg caacagaagc tgcaaatgct tcgggatcat ttctcaattg
     5401 atggcgttga tgccatacac catggtgttt tgtggatgtt tagcagtgaa aatgttaaaa
     5461 tacaagaatt ggcagaatcc attttagcta ctaatattat ctttaaccct aatgctcata
     5521 tctgctatga ataccaatgt ccctagtcaa gaggatcttc ttgctgcgtt gcctttttgc
     5581 ttaaccgaaa cctctttacc tttcggcaaa aagtataaag ggaaagtaag ggatacctat
     5641 gatctcggcg atcaattgat tttggtaact acagatagac aaagtgcctt tgatcgatgc
     5701 cttgctgctg taccttataa aggacaagta ttgaatttga cttcagcatg gtggtttaaa
     5761 aacacgcaat cgattgtgcc taaccattta attgccgtac ctgatcccaa tgtggcgata
     5821 gccaaaaaat gcaaaatatt ccctattgaa tttgttgttc gtgggtatat ttcggggagt
     5881 acaagtacgt ctttatggac gcaataccaa aaaggagttc gtgagtactg tggtataacg
     5941 tttcctgatg gattaagaaa aaatcaaaaa cttgagagcc cagtgataac ccccactact
     6001 aaagagacgc tccatgacag gccgattagc ccacacgaaa tcgtcgcaga aggctggatg
     6061 acccaagaag attgggatga aaccagcagt tatgctttaa agttatttca acatggaatg
     6121 gaagtagcac agcaacatgg tttgattttg gtggatacca aatatgaatt tggccgtgat
     6181 gcggaaggaa gaattgtatt agtggatgaa attcatactc cagattccag tcgttattgg
     6241 ttatttaacg gttatcaaga gcgatttgat gccggtaagg agcctgagaa tatagataaa
     6301 gaatttttgc gcttgtggtt tgttgatcac tgcgatcctt ataaggatga agtattacct
     6361 caagcaccac aggagttaat tgttacttta gcatcaagat atatccaatt atatgaaatg
     6421 attacaggtg aatcgtttgt gtatgacagt aatcctgggc cagtaaatga taggattttg
     6481 cataatatac aacgttggtt aggttaattt ttagccgggg gaaaggctta tgtgtggcat
     6541 tgtaggaatt tacagtcatg aacccgttgc tgctgagctt tatgatagct tgattcattt
     6601 acagcaccgg gggcaagatg cctcaggtat tttaaccgga gctgatcgtt tttacttcaa
     6661 acatggactt ggactggtca gagagtctat tttctctgaa gatattctta aactgaaggg
     6721 gaatatcggc attgctcatg tgcgttatcc cacggccgga ggttatagtg aagcggatgt
     6781 tcagcctctt tggataggga gccctcgcgg tatagctttg gctcataatg gaaatttagt
     6841 gaattatgga gagttggttg atgatttatg cggaaaacag cataggcatt taaactctac
     6901 tcttgattct gaggcacttt tattacttct ggcagactat ttagccaagg gatcttattc
     6961 cgatcatgat gaagagcaat tctttcaatt actatgtatg gcagttaatg aaatacatca
     7021 acgagttcag ggttcctatt ctgttgtgag tttagtaatt ggcaaaggtt tggttgcatt
     7081 tagggatccg catggtatcc gtcctttagt ttggggtgag cgagtccatg aaaatggtag
     7141 taaagattat atttttgctt cagaaaccac ccctttttat gctttaggtt ttgagcccaa
     7201 gggtgatttg cagcccggtg aagttgctta cgtgaatcag caaggacaat tgtacagaaa
     7261 agtgctttca tcaaaaaaat ttaccccttg tatatttgaa tacgtttact tcgccagacc
     7321 agatgccaca ttagacgatg tgagcgttta tcgagccaga ttacgtatgg ggcaaaattt
     7381 agctaaaagc tggaaaaaga gatttccaga tacactacct gatgtggtga ttccagcccc
     7441 ttcaacagca aatactgccg cattagcttt tgcaaatgag ttgggaatac gttattccga
     7501 aggtttatat aaaaatcctt ttattggacg tacttttatt atgcctaatc aggagtcgcg
     7561 atcaagacag gttcgttata aattaactcc tcagaaaacg gaaattaata aaaaaaaggt
     7621 attaatcgtt gatgacagta tcgtaagggg aactacctca cgcgaaatag tcagaatgat
     7681 acgagaatat ggtgcatcag aaatatattt tgtatcaacc tgtccaccaa ttaaaaaccc
     7741 ttgtttctac gggattgata tcccatcgcg taaaaaattg atagcagccc ataaaacgga
     7801 agaggaaatc cgtcaatttc taggggtgga taaattatta taccaaactc aggaaaacct
     7861 tatagaggct gtgtctcgca gggggcaata tgacatgagc aagccttgta tggcttgtat
     7921 ggatggtaaa tacatttgtg gacagattac tgaagaaaaa attaaattgt tggagaaaaa
     7981 aagattaacc gagaatgacc aagaaacaga gataggagag atgtcttgat caatatcctt
     8041 attgttggtt caggtgcgcg tgagcatgca atagctacag cattgagtaa atcagagaat
     8101 aaaccaaaga tttattgctt tggaacaact cgtaatcctg gtatccaaag actcacctca
     8161 gattattggg taggtgatat tacagaccct gagcaagtta accagcaagc aaaagaatgg
     8221 gaaattaatt tggccattat tggtccagaa gcgcctttgg aacatgctgt tgctgatgct
     8281 ttgtgggatt gtggaattcc tgttataggc cccaggaaaa atttggctaa aattgaaacc
     8341 agtaaagcgt tcgccagaga gttgatggaa aaatacggta ttccaggttt gcctaagtat
     8401 aaagtattta ccagtttaga gggaataggg gagtttttat cagagttagg tgaaaataat
     8461 tacgttgtta aggccaatgg tttaatggcg ggcaagggtg taaaagtagg tggagaacat
     8521 ctgttgtcat ttgaagaggc tttaaactat tgtaaagaaa tttttactca taatcagtct
     8581 ttagtcattg aagaaaaatt aataggtcaa gaattttcat ttatgtgttt ttctgatggc
     8641 atctcattag ttccaatgcc tattgtgcaa gatcataaac gagcttatga gcatgatgag
     8701 ggtcctaata caggtggtat ggggagttat tcagcggcag atcacagctt gccatttttg
     8761 tctccagagg atgttcgtgc tgcttttgaa attaatcaat cagtaatcaa agcgcttatg
     8821 acggaaatgt ctgataaata cataggaata ctttatggcg gatttatggc caccgcacat
     8881 ggggtgtatg tgattgagtt taatgctcgc tttggagatc ctgaggctct taacgtgctt
     8941 agcttgttgg aatctgattt tgttaaatta tgtgttgcga tggctaacgg caatttacaa
     9001 aaagaatccg ttcgcttttt gaatcaagcc acagtttgta aatacgctgt ccctgatggt
     9061 tatcctgata atccattgcg ccatgttgaa attgatattt ctgctgtcga acctaaagat
     9121 actctatatt tggctgcagt gaattttaag aatggcaaac tctatgccac aggttcgagg
     9181 acagccgctt atgtcggtat agcgaatact atcagtgagg cagaagaaat tgctgaacaa
     9241 cagataaaaa atataaaagg ccctttattt cacagaaaag atattggcac atcgccttta
     9301 attaatacac gcgttgaagt aatgcgtagg ttgcgtgcct tatgattcgt ttaggaatat
     9361 taggatcgac tcgtggaacc aatatgttgg ctcttgttga tgcaatcaat gagggaactc
     9421 ttaaggcaaa gatagagctt gttattagta ataaacccga tgccattatt ctggaaagag
     9481 ccaaatcgtt gggtttgaat gcacagtttg tcaatcctga gggattaaac agaattgatt
     9541 ttgataaaaa agtaagtgac atactgatca accatcagat tgatttgatt gttttgattg
     9601 ggtacatgag aattttgtcg gcggatttcg taaataaatg gaataatcag gtaattaatg
     9661 tacatccctc tttattgcca gcatttgcag ggaaaatgga tatggatgta catcaagctg
     9721 ttcttgattc cggcctaaag gaaacaggtt gtactatcca ttttgtcacc gaagaggtag
     9781 atgcgggtcc tgttattttg caaaaaaaat gccctgtttt agaaggagat actgctcaaa
     9841 ccttaaaagc aagggtgcag cagttggaag gaatagcttt ggtagatgca attaacctga
     9901 ttgcatcgaa aggtctctaa tgcgagattg tatttccagt caaagcagta ttgtcttcat
     9961 ttccatgaga atagtttttt tctttttcaa ttgtaaaaaa agtgtttaac gaagtttctt
    10021 taaactcaaa taaatggatt ccattcagtt taatactggt gtctgtatgg actcgattat
    10081 ttacagcaga ttttaaaaca tgggaatata atttttcgcg taaactatcg caaacactcg
    10141 gtctattttt agaaaaatat tgattacaat agtttttggc aattaattga aaatcttctt
    10201 tagttaatgt ttgtcgtttt tcctgttcaa ggttaacaac aacaacatca attggagcat
    10261 ttaattcatt accatttttt cttacgataa taatgagatg cggtttttca gggagtcgaa
    10321 ccaactcact ggagaatccg ggtagtgatg caggtagt
//
LOCUS       DACTJE010000112        10285 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.112, whole genome shotgun sequence.
ACCESSION   DACTJE010000112 DACTJE010000000
VERSION     DACTJE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.112"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            149..2458
                     /gene="lem7"
                     /locus_tag="I9760_002124"
     CDS             149..2458
                     /gene="lem7"
                     /locus_tag="I9760_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem7"
                     /protein_id="HAT5043887.1"
                     /translation="MADSRSSIQSILEVDKRFDEKYYNYLVDDNYLIPAEAAVIANGL
                     AIFKEEFESGNLFKQRKDNHDSFTHEQILQYYLELALTCGRLEIIKYLIDELHVSLSE
                     EKLLHFLFLHSPYEGNKYFENIVEIYNYLEKKKEIGSNKQLLEEIFLKEAKKGHFNPV
                     KAIIELKIIPNINITDPDGHTAFFYCILESKKKRFSFNPEELGAVDVILSVRDKEILE
                     LIELVDATILAMEDKNEEKLVKLRRVFTDSIDKIPEALIEDCLSRAIESNDVKMIQLL
                     LPVCNPDLKFLMLGKVNSVEAGMLLIEHFISTGFVIPEARSLEGYAHRLGLNEYTAGA
                     EQANRFLLHAVAGFVNRTDNSKDFKTVLRALQKTVLLNGIANVEVARKILYQTYLEGE
                     PLLLTVGWTQHGVGLSILWDKESNKTYLVFSNRGQGGIASCLNDKGLIKKEEPKAGSV
                     IYEIDGKLDEDFFSHFEIGENNLGLFRYGKKAFEKDIKTCLKDARVLPPFIFAAAQNY
                     ATCGYVNTKQSIRGLLCVFAMTRGDSLSEELIDKMSRQYKEFTTHDRNVACSELIEDF
                     QRKIKFDSRYIQKEYQDFIVKLILSHCTSDKPEDIERTQRLFVVLPEEIQEKLAYLIP
                     CHIVARKADKSSTLQNLSLFKHQEEPSSQNDCFLHRPIGVIPDSVDNLLNKYHEMNTL
                     LKFLESQGYTEEDIPKIVLENNSVRIHTKSDRDFKYKVKDLFPKDIQIVNVQGRYEFI
                     LKDLTEVSYVLNKLKGQTPANNSDPDFGL"
     gene            2588..4069
                     /locus_tag="I9760_002125"
     CDS             2588..4069
                     /locus_tag="I9760_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAT5043888.1"
                     /translation="MNAYQQLEQHFKKYYDFENLAAIVSWDEATMMPAGGGQARAEAL
                     ATLSAVQHEWLTNKKIGDWIKRAKDLPGLSLWQRRNVYWIDRQYQQATCIPAQLIEEF
                     TNESLICVQAWRELRAKNDWRSFKPYFKKLFEKVKMIADIKSQRFNKPCLDVLIDEYS
                     PDLDCKAITPVFDILKQEIPAIIPNVIEKQKSRDIKVISGDFSAEKQKQFGIEIMSRL
                     GFDFNRGRLDVSHHPFCGGISDDVRMTTRYSEQEFISSLMAITHETGHALYEQGLLKE
                     WSRQPVGHSLGMSVHESQSLLIEMQICRSLEFISFLVPIAKKYFKEAQNITEENLYFH
                     CTKVQPDLIRVEADEVTYPLHVILRYEIEQLLFSSEITIDDLPEVWDNFMRKYLGLST
                     KGNDKNGVMQDVHWPSGNFGYFPAYTFGALIAAQLFSAAKKAIPDINDQIKSGDFQSL
                     LIWLRENVHQKAKLFSYQELLRQATGEELNPQYYLAHIRQRYL"
     gene            4130..4633
                     /locus_tag="I9760_002126"
     CDS             4130..4633
                     /locus_tag="I9760_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5043889.1"
                     /translation="MLFFSSAMSKHKLTFTKLSDCMKYLDLAATWAEEEWGYIRNKGL
                     EFRKKILSDLKEHTYIGTFNGQPIAMFVLFDKEMSPEFNHGRFKPPVVNELMYVYIDK
                     PYRGLGFGKQIIDEAKQLASDSKSEYIMLDTLKPGLNRFYEKSGAKVIAENQLFSHPT
                     DVLTIKI"
     gene            4923..6434
                     /locus_tag="I9760_002127"
     CDS             4923..6434
                     /locus_tag="I9760_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1148 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043890.1"
                     /translation="MYMDLGSDNMALSKEIQNKSLRSLIQKYARQLNETDKKLIELIN
                     NLAAADDIPLYAQNQNVINLLIKTIKDSQSEHNPDLEGTSYFIYGLITAEMEAQSEIN
                     RQRHLMQGRFDETALHLRTERDQMQDHDMTLLMPKSQGRIVVMAVLNRYDSHSANAII
                     ETLASDVFNPEVHYIMIPVGPGHWRGVYLSKPQGGTSDTAYDLELFDPYGPEGAAVLD
                     DYVLDLLNQCGVPKELVNIRHTGPKHPQGDAYSCGDFTCAYSHKKMKEFGAPEGSYNP
                     ILIDTLDNLGNEDNVLRMTTREETRALVDKDRGRQHIHPAVQSMRAADKKEAISSTDK
                     LKTGAKGISFEIENANKSKKATIRQTVKESQLSPAMRTGLIIGGGTFTSGGVGAIIGG
                     LMGFFLLPGLGVLMGAIAGAFIGVGAGLLISGIIANAFWNSTPLNIVKAHESNTEIQR
                     VDLKSDSAGKPDKECSEKDEKPIHSPTLFFTLATQERNTNDMADELSSSGPKT"
     gene            6612..7394
                     /locus_tag="I9760_002128"
     CDS             6612..7394
                     /locus_tag="I9760_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043891.1"
                     /translation="MTVQKYFPWFNEAWQSYSLCRSSLWWPLSYLILTPLAVFIVFGL
                     IIPLGLNQLGWINFDHALINYPYLLLIAGLLYFALFPFLDALYQMIKAHLDGKPVKMN
                     TDDIDFINPNTSKVMGMSMGPILLTIIIMEIAGSFFKPFRALVLLAYVLAIFTPILSV
                     NNPESPMKKGLESLQFALNNKKLVAKVWGLRLLILLSLIFPWVIIGITGEHAILKIIT
                     LIAGLPFFFYSLVKLLPFYFFYPAYVYHQAKHQNRHGNISHG"
     gene            7497..8561
                     /locus_tag="I9760_002129"
     CDS             7497..8561
                     /locus_tag="I9760_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair protein Ada"
                     /protein_id="HAT5043892.1"
                     /translation="MFEINDQRKKEYYTALLSKNTCYEGIFYVGVKTTGVFCRPTCPA
                     RKPKLENCEFFHTAQEALLASYRPCRRCQPLSHPNQVSDLVKHLVDAVEANPEKRWKD
                     SDFAALSVDVATARRQFKKRFGMTFVAYARARRMGMAMKQIREGDLVINAQIDAGYHS
                     GSGFRDAFAKIMGAAPSHFNEQHKVLKASWLDTPLGPMLAVADENALYLLEFVDRRGL
                     EREVERLRNKTKAVIIPGDSPVLKAIQKEMELYFSGELRQFNTPIHLIGSPFQKKAWL
                     ALIDIPYGETRSYLQQAKVIGNPSAYRAVANANGANQLAIVVPCHRIINANGELGGYG
                     GGVNRKHWLLEHERRHDRGC"
     gene            complement(8638..10110)
                     /locus_tag="I9760_002130"
     CDS             complement(8638..10110)
                     /locus_tag="I9760_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043893.1"
                     /translation="MAHDKTFFSQQAHSSLTLVNYVHHDGIGDFRHLLDFVSFFNNTP
                     VLKEIELICIVINSHKPGNPQSVYIKEQLESMNVTHKILIYDSINGHEDLRQEIQKNR
                     PLRKQLQHTIGIIDIALNTSYKLVIQEFCKNKPPTITIAEIDLYATYDQPSLASDPEK
                     RIDKSRYQADHVYIMGLGTGKVGLKLTGGLFNSPQKAREALQQISDKRIKNLLLDQVA
                     EEPISQETINVFLQNNHFIPGYLQDIDTIFGFISIFATNHKLNEERKNLIFYLNNYQN
                     LFKKNYYDEDTGRRYSQFTDLLFDKHLPWHKVFASSGIRSITIYNYAGQKPTEEEITL
                     NPEGQQMTIITGIMLNDTDYQLLYDIPQHFVAASGDNTLENAINHGLLFLMQYKYQYH
                     TVREIAKIAEQAKDTLGFSEEEIREFHAYFGEAHHLISYSNEKSTKARSLLQSIDLIR
                     LSSNMRKVCAYLIENENFLHQLPEILQTDLLNISKPGMKQ"
BASE COUNT         3294 a         1646 c         2091 g         3254 t
ORIGIN      
        1 ataaaaaaac aaaattattt ttacagggag tggaatattt ttaaaaattg cttcaaatga
       61 tcgcatagtc gcatgaaaaa aaccaaaaat ctctgactgg ttattttttg attatactta
      121 aagataacca tcaagaggag ataaaatttt ggctgattcc agatcatcga ttcaaagtat
      181 tttggaagtc gataaacgat ttgatgaaaa atattataat tatttggttg atgataatta
      241 tttaattcca gctgaggctg cagtcatagc caatggatta gcaatattca aagaagagtt
      301 tgagagtgga aacttattta aacagagaaa agacaatcat gactctttca ctcatgaaca
      361 aatccttcag tattatttgg agttggctct tacttgtggg cgccttgaga tcattaaata
      421 ccttatcgat gagcttcatg tttctttatc cgaggaaaaa ctgttgcact ttctattttt
      481 gcacagtcct tatgaaggaa ataaatattt tgaaaacatc gtagaaattt ataattattt
      541 ggaaaagaag aaggaaattg gaagtaataa acagttgtta gaagagatct ttttaaagga
      601 agctaaaaaa ggtcacttta acccggtcaa agcgattata gaacttaaga ttataccaaa
      661 tataaatatt accgaccccg atggccatac agcatttttt tactgtattt tggaaagcaa
      721 gaaaaagaga ttttccttta atcctgaaga gttgggagca gtggatgtta ttttatcagt
      781 tcgggataaa gaaattcttg aattgataga gctggttgat gcaaccattt tggcaatgga
      841 agataaaaat gaagaaaaac tggttaaatt aaggagagtg tttactgact caatagataa
      901 aattcctgag gcgttgatag aagactgttt gtcgcgagca attgagtcga atgatgtcaa
      961 gatgattcaa cttctgttac ctgtttgtaa tcctgatcta aagttcttga tgcttggtaa
     1021 ggtcaattcc gttgaagccg gtatgctact aatagagcat tttataagta ctggatttgt
     1081 tatacccgaa gcaaggagtt tggagggtta tgcgcacagg ttagggttaa acgaatacac
     1141 tgctggtgcc gaacaggcga atagattttt gttacatgca gtcgctggat ttgtcaatcg
     1201 gacggacaat agtaaagact tcaagactgt tttaagggcg ctacaaaaga cagtgctatt
     1261 aaacggcata gcgaatgtgg aggtggcgcg gaaaatttta tatcaaacgt atttggaagg
     1321 cgaacctcta ttgcttacgg tgggatggac gcaacatggt gttggtttat ccatattatg
     1381 ggataaagag tccaataaaa cttatcttgt attttctaac cgagggcaag gaggaattgc
     1441 ttcatgtctt aatgataagg gtttaattaa gaaagaggaa ccgaaagcag gttcagttat
     1501 ctatgagata gacggtaagc tggatgaaga ctttttctct cattttgaga taggagaaaa
     1561 taacttgggt ctttttagat atgggaagaa agcatttgaa aaagacatca agacatgtct
     1621 taaagatgcc cgagtattac ccccctttat ttttgctgca gcacagaatt atgccacctg
     1681 tggctacgtc aacacaaaac aatccatacg cggtttatta tgtgtttttg ctatgacaag
     1741 aggcgattct ttaagtgaag agttaattga taaaatgagt cgtcaataca aagaatttac
     1801 aactcatgat agaaacgttg cttgttccga gttaattgaa gattttcaga ggaaaattaa
     1861 gtttgattcc aggtatattc agaaggagta tcaagatttc attgtgaaat tgattctatc
     1921 gcattgtaca tcggataaac cagaagatat tgagcgaacc caacgattat ttgtagtgtt
     1981 gccagaagag atacaggaaa aactggctta tctgatacct tgccatatcg tagcacgaaa
     2041 agcagataag tcatcaaccc ttcaaaatct gagtctcttc aaacatcagg aagaaccttc
     2101 ctcccaaaat gactgttttt tgcataggcc gatcggagta atccctgatt ccgtggataa
     2161 tttactgaat aaatatcatg agatgaatac actacttaaa tttttagagt cccaaggtta
     2221 tacagaagaa gatattccga aaattgtttt ggaaaataat tcagtccgaa ttcacactaa
     2281 gtctgataga gattttaagt ataaagtaaa agatcttttt cccaaagata tccagattgt
     2341 taatgttcag ggaagatatg agtttattct caaggatctg acggaagtca gttatgtatt
     2401 aaataaatta aagggccaga caccagcgaa caatagcgat ccggattttg gtctataata
     2461 ttttttcatg agtcagtttt taaatttctt gaaaggattt taactatgtt attctgtatg
     2521 tggtgctttg aaagctatca gatctgagtg gaacccatat ttttaaagta taagccaaga
     2581 gtccttcatg aatgcctatc aacaattaga acaacatttt aaaaaatatt acgattttga
     2641 aaatttagca gccattgttt cctgggatga agcaacgatg atgccagccg ggggtggtca
     2701 ggcgcgtgca gaagcgttag ccactttaag tgctgtacaa catgaatggt taactaataa
     2761 aaaaataggt gactggataa aacgggcaaa agatttacca ggtttatcat tatggcagcg
     2821 acgaaatgtt tattggatcg ataggcaata tcagcaagca acctgtattc cagcgcaact
     2881 gatagaagag tttacgaatg aatctttgat atgtgttcag gcatggcggg aattacgagc
     2941 taaaaatgat tggcgctcat ttaaaccgta ctttaaaaag ttatttgaaa aagtaaaaat
     3001 gattgccgat attaaatcac aaagatttaa taagccttgt ttggatgtct taatcgacga
     3061 atactccccg gatttggatt gcaaggccat caccccggtt tttgatatct tgaaacaaga
     3121 aataccagcc attattccca acgttatcga aaaacaaaaa agtcgagata ttaaagtgat
     3181 ttcaggtgat ttttctgcgg aaaagcaaaa gcaatttggg attgaaatca tgtctcgatt
     3241 ggggtttgac tttaatcgtg gtcgattaga tgttagccac cacccgtttt gtggtggtat
     3301 ttcagatgat gttcgaatga caacccgata cagtgagcag gaattcatat cttccttaat
     3361 ggcaattacc catgaaacag gtcatgctct gtatgagcaa ggcttgctaa aagaatggag
     3421 tcggcaacct gttggccatt cattaggaat gtctgtgcat gagagccaat cgttgcttat
     3481 tgagatgcaa atttgtcgaa gtttggagtt tataagtttt ttggttccta tcgcgaaaaa
     3541 atattttaaa gaagctcaaa atataactga agagaatctt tattttcatt gtacgaaagt
     3601 acaacctgat ttaattcgtg ttgaggcaga cgaagtcact tatccactac atgtaatact
     3661 gcgttacgaa atcgaacaac ttttgttttc ttcggagatt accattgatg atttaccaga
     3721 ggtctgggac aacttcatga gaaaatattt aggactatca acaaaaggga atgacaaaaa
     3781 tggtgtgatg caagatgtgc attggccttc tggaaatttt ggctattttc ctgcttatac
     3841 gtttggtgca ttaattgccg ctcaattgtt ttcggcggcc aaaaaagcaa tccctgatat
     3901 caatgatcaa atcaaatcag gtgactttca atcgttgtta atttggctta gggaaaatgt
     3961 tcatcaaaaa gcaaagttat tttcttatca agagttattg cgtcaagcaa caggcgaaga
     4021 gttaaatccg caatattatt tggctcatat cagacagagg tatttataaa acggggtggc
     4081 cttagataaa atttgcaggt tgtatcattt taattaagag ggttatcaga tgttattttt
     4141 tagttcagca atgtccaaac acaaactcac ttttactaaa ctgtctgatt gcatgaaata
     4201 tttggatctt gccgcaacat gggcagagga agaatgggga tatattcgta ataaagggct
     4261 agagtttcgt aaaaaaatcc tgtctgattt gaaagagcat acttatatag gaacttttaa
     4321 tggacaaccc attgccatgt tcgttttatt cgataaagag atgagtccgg aatttaacca
     4381 tggaagattt aaacccccgg tagtgaatga gttgatgtat gtctatattg ataaaccata
     4441 tagagggctg ggttttggaa agcaaattat tgatgaggcg aaacaattag caagcgattc
     4501 aaaatcggaa tacattatgt tggatacttt aaaacctggc ttgaatcgat tttatgaaaa
     4561 aagcggggca aaagtgattg cagaaaatca attgttttcg catccaactg atgtattaac
     4621 aataaagata taagctgtga tctctatcac gggtaagtaa tgctcttttt tatgtggaca
     4681 tgagcatgtt atcgtgttta agttatcata tattcaatgg gtgcaataat taatcatgag
     4741 agtgaatttc ttttcggtta attatttttt atttcattag acctcttgcc ctaaaacaaa
     4801 agagacaggt taggcaagag gtctatttca gagtcaaaag cattgcgtaa ctgcttcagg
     4861 tggagcatac attattaaag aggagggaag ctttattttt tcttaattgt tttatatagc
     4921 atctgtatat ggatttaggg agcgataata tggcattaag taaagaaatt cagaataaaa
     4981 gtttaagatc attaatccaa aaatacgcca gacaacttaa tgaaacagac aagaagctca
     5041 tagaacttat taataatctc gctgcagcag atgatattcc tttgtatgcc caaaaccaaa
     5101 atgttatcaa tttgttaatt aaaaccatta aagacagcca gtctgaacac aatccggatt
     5161 tggagggaac ttcctatttc atttatggat tgatcactgc agaaatggag gctcaatctg
     5221 aaataaaccg ccaaagacat cttatgcagg gacgttttga tgaaacagca ttgcatttaa
     5281 gaacagagcg tgatcaaatg caagatcatg acatgactct gctcatgcca aaatctcagg
     5341 ggcgtatcgt tgtgatggca gttctcaatc gctacgactc tcattctgct aatgcgatta
     5401 tagaaacttt ggcaagtgat gtattcaatc ctgaagttca ttacattatg atacctgttg
     5461 gacccggaca ttggcgagga gtttatttaa gcaaacccca aggcggcact agcgatacag
     5521 cttatgatct tgaattattt gacccttatg ggcctgaagg cgctgcagta cttgatgact
     5581 atgtacttga tttattaaac cagtgtggcg ttcccaaaga gcttgtgaat atacggcata
     5641 cgggtccgaa acatccgcaa ggtgatgctt actcatgtgg ggattttact tgtgcctata
     5701 gccataagaa aatgaaagaa tttggagctc cagaaggtag ttataatccg attcttatcg
     5761 atacgctgga taatttaggt aatgaagata atgtgcttcg tatgacaacg cgcgaagaaa
     5821 cgcgagcgct tgttgataaa gacaggggaa gacagcacat tcatcctgcc gttcagtcta
     5881 tgcgggctgc cgataaaaaa gaggcaatat cgtccactga caaacttaaa acaggagcaa
     5941 aagggatatc ctttgaaatt gaaaatgcta ataaatcaaa aaaagcaact attcgccaaa
     6001 ctgttaaaga aagtcaatta tcaccggcta tgcgaacagg tcttattata ggtggaggta
     6061 cattcacctc aggaggagta ggggctatta ttggcggact catgggtttt ttccttttgc
     6121 caggactggg tgtattaatg ggggcgatag cgggagcttt tattggtgta ggagcaggtt
     6181 tgttgatatc aggtattata gccaatgcat tttggaatag tactccatta aatattgtaa
     6241 aagcccatga gagtaacacc gagattcaaa gagtcgattt aaaatccgac tctgcaggta
     6301 aacctgataa agaatgttcg gaaaaggacg agaagcctat ccacagccct actttatttt
     6361 tcactctcgc tacgcaagag agaaacacaa atgacatggc cgatgagttg agttcaagcg
     6421 gtcctaaaac atagaaaaac aatcataaat ttattttatg gataccgctg gcaagcagcg
     6481 gtacgtaggc ggtgaggatg aattgttaac atacctttca atatgtcaat tcagcatttg
     6541 tgagtattct tttaactatg gaattaacgt gctaatataa atcatttaat ataattagtg
     6601 tccgacattt catgactgtg caaaaatact tcccttggtt taatgaggca tggcaatctt
     6661 actcactttg ccgctcttcg ctatggtggc ctttaagtta tcttatatta acaccacttg
     6721 ctgtctttat tgtttttgga ttaattattc cattagggtt aaatcagtta ggttggatta
     6781 attttgacca tgcgttgatt aattatccct atcttttgct aatagcaggg ctactttatt
     6841 tcgctctttt cccttttctt gatgcattat atcagatgat taaggcccat ctggatggca
     6901 aacccgtcaa aatgaatacg gatgatatcg attttattaa tcccaatact tcgaaagtaa
     6961 tgggcatgtc tatgggcccc attttattga ctattattat catggagatc gctggctctt
     7021 tctttaaacc tttccgagca ttggtgctgt tagcttatgt attggctatt tttacgccta
     7081 ttttaagtgt aaataatcct gaatcaccta tgaaaaaggg actcgagtcc ttgcaatttg
     7141 ctcttaataa taaaaaattg gttgccaagg tttggggttt acgtcttttg attcttctgt
     7201 ccttgatctt tccctgggtt attataggga ttactggtga gcatgcgatt ttaaagatta
     7261 ttactctaat agcaggatta cctttctttt tttattcttt agttaaatta ttgccttttt
     7321 acttctttta tccagcctat gtttatcatc aggcaaaaca tcaaaacagg catgggaata
     7381 tttctcatgg ataactggcg ccttttctaa ttttaaaaat tcactgtctg attttcggct
     7441 tcaccatttt aaagaagatt tataataatt agtaagaaag attgtcagga atggtgatgt
     7501 ttgaaattaa cgatcaaaga aaaaaggaat attatactgc tctgttgagt aaaaatacct
     7561 gctatgaagg gattttttat gtcggagtga aaacaacagg tgttttttgt cgaccaactt
     7621 gtcctgcaag aaaaccaaaa ttggaaaatt gtgaattttt tcataccgct caagaggctt
     7681 tattagcctc ttacagaccc tgtagacgtt gtcagcctct gtcacatcca aatcaagtct
     7741 ctgatttggt taaacatttg gttgatgcag ttgaggctaa tcctgaaaaa agatggaaag
     7801 acagtgattt tgcggcctta tcagttgatg ttgcaacagc aaggcgccaa tttaagaaaa
     7861 gatttggcat gacatttgta gcatacgctc gtgccagaag aatgggcatg gcaatgaaac
     7921 aaatcagaga aggtgattta gtgattaatg cgcaaattga tgctggatat cactcaggta
     7981 gtggttttag agatgctttt gcgaagatta tgggagcagc tccatctcat tttaatgaac
     8041 agcacaaagt tcttaaggct tcctggttgg atacaccttt aggtcctatg ctggctgttg
     8101 cagatgaaaa cgctttatat ttacttgaat tcgtcgatag aagagggttg gaaagagaag
     8161 ttgaaaggct gagaaataaa accaaagcag tcattatccc gggagattcc ccagtgttaa
     8221 aggctataca aaaagagatg gagctttatt tttcaggaga actaagacag tttaatacac
     8281 ctatccattt aattggcagc ccttttcaaa aaaaggcatg gcttgcgtta atcgatattc
     8341 cctatggtga aacaagaagt tatctgcaac aggcaaaagt aataggtaat ccgtcggctt
     8401 acagggcggt ggctaatgcc aatggtgcta atcaattagc tatagtggtt ccttgtcatc
     8461 ggattatcaa cgctaatggc gaactgggag gatatggagg tggtgtaaat cgtaaacatt
     8521 ggttacttga acatgaaaga agacatgatc gaggttgcta aactaatttt gctacgctca
     8581 aaaaggagta tagaataaac atgttaacct accagaagct gtagtgattg ataaaattta
     8641 ttgtttcatt cctggtttgc ttatattcaa taaatcggtt tgtaaaattt caggtaattg
     8701 atgtaaaaaa ttctcgttct ctattaaata ggcgcaaact tttctcatat tggaagacaa
     8761 acgtattaaa tctattgatt gtaacaaaga tctggctttt gtagactttt cattactata
     8821 actgataagg tgatgggcct caccaaaata ggcatgaaat tctcgaattt cttcttcact
     8881 aaaacctaaa gtatcttttg cttgctctgc aattttagca atttctctaa cggtatgata
     8941 ttgatactta tattgcatta aaaatagcag accatgatta atagcatttt ccagcgtatt
     9001 gtcacctgaa gctgcaacga aatgttgtgg gatgtcatag agcaattgat agtcagtatc
     9061 attaagcata ataccagtaa tgatagtcat ttgctgacct tccggattta gggttatttc
     9121 ttcttcagta ggcttttgac cagcgtagtt atagatggta attgagcgga tacctgaact
     9181 tgcaaaaact ttatgccaag gtaaatgttt gtcaaacaat aaatcggtga actgtgagta
     9241 gcgtcgcccc gtgtcttcat cataataatt tttcttgaat agattttgat aattgtttaa
     9301 ataaaaaatg agattttttc tttcctcatt taatttatga ttggtggcaa aaatactgat
     9361 gaatccaaat atagtatcga tatcctgaag gtacccagga ataaaatgat tattttgtaa
     9421 aaaaacgttt attgtttctt gtgaaatggg ctcttcagca acttgatcta acagtaaatt
     9481 tttaattctc ttgtcactga tttgctgcaa agcttctcgg gctttttgag gagagttaaa
     9541 taatcctccg gttaatttta gccctacctt tcctgtcccc agccccatta tgtagacgtg
     9601 atcagcttga tatcttgatt tatcgatacg tttttcagga tctgaagcca gagatggttg
     9661 atcataagta gcgtataaat caatttcggc tatagtgatt gttggtggtt tgtttttgca
     9721 aaactcctga ataaccagtt tataagaggt attcaatgca atatcaatga ttccaatagt
     9781 atgctgtaat tgttttcgta atggtctatt tttttgaatt tcttgtcgta aatcctcatg
     9841 accattaatg gagtcgtaga tcaaaatctt gtgtgtgaca ttcatgcttt ccaactgctc
     9901 ttttatatac acagattgag gattacctgg tttgtgactg tttataacaa tacaaatcaa
     9961 ttctatctct tttaaaacag gagtgttatt aaaaaaagac acaaaatcaa gtaaatgcct
    10021 gaaatcacca attccgtcgt gatgcacata attaaccaaa gtaaggctag agtgtgcttg
    10081 ctgagaaaag aaggttttat cgtgtgccat tattctgaat aaatagtaat cataataata
    10141 aatatagaca atgttagtca gtaaatatca actaatgatt taaaatcttt taatcctgtg
    10201 cttgagaatt ttggttataa aatctgaagt gatctctgat tattttttcc caagcatggg
    10261 aagaagtggg ttctttttag cagaa
//
LOCUS       DACTJE010000113        10241 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.113, whole genome shotgun sequence.
ACCESSION   DACTJE010000113 DACTJE010000000
VERSION     DACTJE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.113"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(146..514)
                     /locus_tag="I9760_002131"
     CDS             complement(146..514)
                     /locus_tag="I9760_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HAT5043894.1"
                     /translation="MSVEIQQASNTNPNITFSESAIKHLVTYLEKNPGNKGVRLSVKK
                     TGCSGLSYVVDYVKEPSEGDMIASLSKDYLVCVDKTSYPFLKNMQVDYVKQGLNYKFI
                     FNNPNQTGQCGCGESFTVSQ"
     gene            complement(519..902)
                     /locus_tag="I9760_002132"
     CDS             complement(519..902)
                     /locus_tag="I9760_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043895.1"
                     /translation="MMYNKIVQDYFFQPQHIGKLDLNDPFVIGVQSQPINQLNTVHFY
                     IKCDGDKLISKARFKTNGNPYVIAAMEWICCQIEGKTLESLPLIDFQILINVLQITNN
                     QYPVAVQVEDIYKEALTLMRKKFER"
     gene            complement(899..2068)
                     /locus_tag="I9760_002133"
     CDS             complement(899..2068)
                     /locus_tag="I9760_002133"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HAT5043896.1"
                     /translation="MELAIKPIYFDYMATTPVDPRVVEQMIKYLGPEGDFGNPASATH
                     EYGRVASMAVEQARSQIAETINASPQEIVFTSGATEADNLAILGAARFYKNKGMHLVT
                     MSTEHKAVLDSFHQLEKEGFQVTYLNPESDGLLDLEKLESALRLDTILVSVMHVNNEI
                     GVIQDIASIGELLRNRGIIFHVDAAQSAGKLPIDLSQLSVDLMSFSAHKNYGPKGVGA
                     LYVRHKPRIRLQPLSYGGGHEGGLRSGTLPTHQIVGMGEAFAIAEADRIPEQTRILNL
                     RKQLWDGIRHLPAIKLNGHEHRRIAGNLNVSFVGLNGDSLLFALSELAISTTSACSSA
                     SIQPSYVLRAIGLTDTEAQSTIRLSIGRFTSEVQIKKAIDIICTQVSRLHELSPL"
     gene            complement(2037..2810)
                     /gene="trmJ"
                     /locus_tag="I9760_002134"
     CDS             complement(2037..2810)
                     /gene="trmJ"
                     /locus_tag="I9760_002134"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAT5043897.1"
                     /translation="MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPD
                     LKAKEMAAGADDVLDAAVVTNTLDEALIGCQLILGTSARPRGLSLPGLIPASCAELIN
                     QQSGNTQVAIVFGREHAGLTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMK
                     LLSPSAQVALRNEEPATADEIEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRIN
                     LEKMEVSILRGMLSQVQKSLEWARKDNRSDRIGNKTDLF"
     gene            2926..3711
                     /locus_tag="I9760_002135"
     CDS             2926..3711
                     /locus_tag="I9760_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HAT5043898.1"
                     /translation="MEPLLNIAVNAARQAGEIINRYVEQVDRLKITPKNSHEYFSEVD
                     IKAEQVIINTIHKAYPEHGILAEESGIQQSDSDTVWIIDPLDGTSNYLHGFPFYSVSI
                     ALKIKNRLEHGVIYDPLRHECFAASRGRGARLNDRRIRVSKQTQLNASLLGTGFHFRD
                     ATLAQRYLPTFEALIGKCAGVRRTGSAALDLAYVASGRLDGFWEFGLRPWDIAAGALL
                     VREAGGLISDVQGGEDFLNYGDVVAGTPKVFKSLLQTISPVLK"
     gene            complement(3824..4780)
                     /locus_tag="I9760_002136"
     CDS             complement(3824..4780)
                     /locus_tag="I9760_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HAT5043899.1"
                     /translation="MNNESSSNSNLDSQALLNQLVIDYLQERKRKRRWKWIIRAIILA
                     VLVYSSYKIVSVAKTTEASKDKEHIGLIDINGEIADSSSASADNFTKGIEKAYRSKGL
                     KALIVRINSPGGSPVQAEYIYNVLQYYKSLKPNIKTYAVCVDMCASAAYYVAVGADEI
                     YASPASMVGSIGVLYNGFGFVDALQKLGISRRLQTSGVNKAFLDPFSPTTEFQKEKLQ
                     TMLDIVHKQFITRVKEGRGNRLHVDDETFSGLFWTGEQALASGLIDGYASSGQLAREV
                     IKITDIVDYTYKQNVFDRLTKNLGTAMADELPVILGMKPGLR"
     gene            4869..7445
                     /gene="clpB"
                     /locus_tag="I9760_002137"
     CDS             4869..7445
                     /gene="clpB"
                     /locus_tag="I9760_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAT5043900.1"
                     /translation="MRMDKLTSKFQMALADAQSLALGRDNGFIEPEHLMKALLDQQGG
                     SCRPLLSKAGVNIPLLRTLIDQALDKLPKVSGTGGDIHISNALNRLLNLTDKLSQQRK
                     DNFISSELFILAAINEDSNLAKILKQAGGDNKAIEKAIDELRGGETVNDPNAEEQRQA
                     LEKYTLDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKNKRLLALDMGALIAGAKYRGEFEERLKGVLNDLAKQEGQIILF
                     IDELHTMVGAGKAEGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVDEPSVEDTIAILRGLKERYEVHHGVEITDPALVAAATLSHRYISDRQLPDKAID
                     LIDEAASLIRMEIDSKPESMDKLERRLIQLKIEREALKKENDEASKKRLVDLQKSIDE
                     LEQNYSDLEEIWKAEKATMQGSTQIKEALEQAKLEMETARRAGDLSRMSELQYGRIPE
                     LEKRLSQVSSVDAMETKLVRNKVTEDEIAEVVSKWTGIPVSKMMEGEKEKLLKMEEAL
                     HSRLIGQNEAVDAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALASFLFD
                     TEEAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HTDVFNILLQVMDDGRLTDGQGRTVDFRNTVIVMTSNLGSQLIQEMSSKFNYDEIKAA
                     VMDLVSQHFRPEFINRIDESVVFHSLTKEQIAKIAAIQINYLHHRLKQQNITIEVTSE
                     ALSHLAEAGFDPVYGARPLKRTIQQKLENPLAQSLLTGKFKSGDTIIVSYKDGVMEFY
                     KQ"
     gene            complement(7537..8847)
                     /locus_tag="I9760_002138"
     CDS             complement(7537..8847)
                     /locus_tag="I9760_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1751 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043901.1"
                     /translation="MTFTPPEFKILSVNTRNLETIFSTLLGRYKIITDPPVSEAVSSG
                     EIIRNTLETLLARTHKVVICKTDRETARDVFKQLSNELREVLKENNEEKNKQAILFLL
                     GALLHRYFRLIKEYDNFNSYIPYPSFLFSKPPSNVKDCRLFQAIRLALGLPEVMERNY
                     RINDLKILDVTTIVTALETFRDNMQLIVGKDEGKMPRYKSYPHFAADKNFETYLQEII
                     DEHKRRNPVVLNQFKAINFIQSLVKQIEEEQRQIEEALTHLGKFLPKTCSDFKTISLE
                     LMEEQIKAQIESNVLQEKIIDLLYTGHIQENFSTMDCGSFIEAMKNCNSSLARYRALG
                     GYCLLLQNEGVKEQLRFCIHQALGVEINPNELTDKDMLDAIRLLKTYFEANPKVELNF
                     DFFNGKGSMNTFILQTELALAKKVQTVNKAQEDNSETRTTSLFV"
     gene            complement(8860..9507)
                     /locus_tag="I9760_002139"
     CDS             complement(8860..9507)
                     /locus_tag="I9760_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1752 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043902.1"
                     /translation="MRFLRVGPCGRETMLEFAEFQQNALKLTDQLKKRSIPSLGCFCF
                     FSVILPNTAAIEDTHTIKLIAYSLDSENDDDLIAYLKGVTTYSAVTPKNINEFKKKVL
                     TGIYLMMWSHYSSTVSNLMNSTLVELFQRDLQVSSPQKMSDTLFDASLEALSQYCSFA
                     YQNRNERIYDNLIRRLGKTVQADIHALRHSREKQETSFYDIYKGIMRTLGLNSVF"
     gene            complement(9800..>10241)
                     /locus_tag="I9760_002140"
     CDS             complement(9800..>10241)
                     /locus_tag="I9760_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214018.1"
                     /note="ligates
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate to
                     UDP-N-acetylmuramic acid for reincorporation into
                     peptidoglycan; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAT5043903.1"
                     /translation="EQFSPVKRRLEVRSNRHGITVYDDFAHHPTAITKTIDALKRSGR
                     HQRIFAVMEFASYTMRTGVHAAEMAHALAPVDGAYVLEPQDFNLKDFVSSWTCPYKIC
                     KNNGEIVKEIIKTVREGDAVLVMSNRGFNGIHQQLIDSIDKLFM"
BASE COUNT         3225 a         2011 c         1878 g         3127 t
ORIGIN      
        1 tgcttttata attagaaatt tttcggggat taataattta actaataaaa agaatataaa
       61 accatgaggc tgtatattgc gactgccctt caagctagcc gcttattgat aatattaaaa
      121 aggcatcgtg gactttctgc ttattttact gacttacagt aaagctctct ccacaaccac
      181 attgccccgt ctgattggga ttattaaaaa taaatttata atttaaacct tgtttcacat
      241 aatcaacttg catattttta aggaatggat aacttgtttt atcaacgcat accagataat
      301 ctttagataa agaagctatc atatctcctt ctgaaggttc ttttacgtaa tccactacat
      361 aagataatcc cgagcaaccc gtctttttta cagacaatct tacccccttg tttcctgggt
      421 ttttctccag ataggtgacc aggtgcttta ttgccgattc actgaaagtg atgttggggt
      481 tcgtattgct cgcttgttgt atttctacac tcattgcttt acctctcaaa tttcttacgc
      541 atcaaagtca atgcttcctt atatatgtcc tctacctgga ctgctacagg atactgatta
      601 tttgttatct gtaatacatt aattagaatc tgaaaatcta ttaatggaag tgattccaaa
      661 gtcttacctt caatctggca acatatccat tccattgccg caataacata agggttacca
      721 ttcgttttaa atctggcttt ggatatcagt ttatctccgt cacattttat ataaaaatgt
      781 actgtattca gttgattaat cggttgactt tgtacaccta ttacaaaagg atcatttaaa
      841 tccagcttac caatatgttg tggctgaaaa aaataatcct gtacaatttt attatacatc
      901 ataacggcga taattcgtgc agccgactca cttgtgtgca aattatatca attgccttct
      961 ttatttgcac ctcggaagtg aagcgtccta tggataaacg aatagttgat tgcgcctctg
     1021 tatcagtcaa accaattgcc cttaagacat aagatggttg aatactggca gaagaacagg
     1081 cagaagtagt tgatatcgct aactcactta aagcaaacag caatgaatct ccatttaatc
     1141 caacaaaact cacattcaga tttccagcga tacgcctgtg ctcatgtcca ttcagtttaa
     1201 tagccggtaa atgtcgtatt ccgtcccata gttgtttgcg taaatttaga atacgcgtct
     1261 gttcaggaat tctatcagcc tctgcaatag caaaagcctc ccccatgccg acaatttgat
     1321 gggtaggtaa tgttcctgag cgcaatccac cctcatgccc gccaccgtaa cttaaaggct
     1381 gtaaacgaat ccttggctta tgcctgacat acagagcccc tacaccttta ggcccataat
     1441 ttttatgagc agagaatgac atcaaatcaa ccgaaagctg gctcaagtcg attggcaatt
     1501 taccagcact ttgagccgcg tcaacatgaa atataattcc cctgtttcgt aataactcac
     1561 caatagaggc aatatcttgg atgacaccaa tttcattatt aacatgcatg acagaaacca
     1621 gaatagtatc taacctgaga gcagattcca gtttttcaag atccaacaaa ccatctgatt
     1681 ccggattaag gtaggtgact tgaaaacctt ctttttccag ttggtgaaaa ctgtcaagca
     1741 cagctttgtg ttcagtgctc atagtaacca gatgcattcc tttattttta taaaaccgtg
     1801 cagctcctaa aatcgccaaa ttatccgctt ctgtagctcc tgatgtaaac actatttctt
     1861 ggggtgaggc attaatagtt tctgcaattt gagagcgtgc ctgctcaact gccatagagg
     1921 caactctccc atactcatga gttgctgatg caggattacc aaaatcccct tcagggccta
     1981 aatattttat catttgctca acaacacgag gatcaacggg tgttgttgcc atataatcaa
     2041 aataaatcgg ttttattgcc aattctatcg ctcctattgt ctttccttgc ccactccaat
     2101 gatttctgaa cctggctaag catacctctt aaaatactaa cttccatttt ttcaaggttg
     2161 attctattaa atagtcgcct cactctttgc attaatctcc ttggattaga gggcttcaaa
     2221 aaattaattt caataaaaac ttctttcaga tgctcataaa attgctctat ttcatcagct
     2281 gtagcaggtt cttcattgcg cagagctact tgagcactcg gtgataataa cttcatacga
     2341 agttcataag cgataatttg tactgcctga gccaaattta atgaactgta ctcaggattg
     2401 cttggaatat tgatatggta gtgacatttt aacagctctt cattagtaag gcccgcgtgt
     2461 tctctaccaa atacaatggc cacttgagta ttacctgatt gttgatttat caattctgca
     2521 caggaagctg gaattaatcc aggtaatgac agtccacggg gtctggcact cgtccctaaa
     2581 atcaactgac atccaattaa agcttcgtcc agggtatttg taacaactgc tgcatccaga
     2641 acatcatcag ctccagcagc catttctttt gctttgagat caggaaatga ctttggatta
     2701 accaggtaca gagtacttag ccccatggtt ttcatggctc ttgccgtaga gcctatattg
     2761 ccaggatggg aagttgaaac caaaacaatt cgaatagaac ttaacttcat aaaaaattat
     2821 tccaaataca atagataact atatcatagg atttattaat tgaacttctc tttgtttaaa
     2881 aaaaatgtgt tagaattgct gattttatag ctaaagagta tatccatgga accactttta
     2941 aatattgctg tcaatgctgc gcgacaggct ggtgaaatca ttaatcgtta tgtagagcaa
     3001 gttgatcgtt taaaaataac tcccaaaaac agccatgaat attttagtga agtggacatt
     3061 aaagcggagc aagttatcat taatacgata cataaagcct acccagagca tggaattctt
     3121 gccgaagaaa gcggtattca gcaaagcgat agtgatacag tctggatcat tgatccattg
     3181 gatggcacct caaattatct tcatggattc cctttttatt ctgtatccat agccctgaaa
     3241 attaaaaatc gtctggagca tggtgttatt tacgatcccc tgcgacatga gtgttttgct
     3301 gcaagcagag gacgcggagc aaggctaaac gatagaagaa tccgtgtgtc caagcaaact
     3361 caactcaatg cttctctgtt aggcacaggt tttcattttc gagatgcaac tcttgcacag
     3421 cgatatttac caacctttga agcattgatt ggaaaatgtg ctggagttag aagaacaggt
     3481 tctgctgctc tcgacttggc ttatgttgca agcggtcgtc tggatggctt ctgggagttt
     3541 gggttgcgcc catgggatat tgctgcggga gccctgctgg ttcgagaggc tgggggatta
     3601 ataagtgatg tccagggtgg agaagatttc ctgaattatg gcgacgtagt agcaggaact
     3661 cctaaagtat ttaagtcact tttgcaaacc atatcacccg ttttaaaata agttgtcaat
     3721 ttaagctcat ttaagacgtt catttaggct tatcgatttt aaattatttt cgaatatccg
     3781 ctctctcgaa gctgagaaag cggataaata ctcctctcca ctcttatctt aatcctggct
     3841 tcattcctaa aattactggc aactcatctg ccatcgccgt gcccaaattt ttagttaggc
     3901 ggtcaaagac attttgcttg taagtatagt caactatatc agtaatttta attacctctc
     3961 gcgccaactg cccgctactg gcataaccat caattaaacc actagccaag gcttgctcac
     4021 cagtccaaaa taacccggaa aaagtctcat catcaacatg taatctattt cctcttcctt
     4081 ctttgacacg agtaataaat tgcttatgaa ctatatcaag catggtttgt agcttttctt
     4141 tctgaaattc agtcgttggg gaaaaagggt ccaggaaagc tttattgact cctgaagttt
     4201 gtaatctgcg tgaaatacca agcttctgca atgcatcaac aaaaccaaag ccattatata
     4261 aaacacctat tgagccaacc atgcttgcag gacttgcata gatttcatct gctccaactg
     4321 caacataata cgcagcagaa gcacacatat cgacacaaac agcgtaagtt ttaatgtttg
     4381 gttttaggct tttgtaatat tgtaaaacat tataaatata ttcggcctgt acaggactgc
     4441 ctccagggct atttatccgt acaattaatg cttttaatcc tttacttcta taagccttct
     4501 caattccttt agtaaaatta tcagcacttg ctgaactgga atcagcaatt tcaccattaa
     4561 tatcaattag gccaatatgc tctttatcct tagatgcttc cgtagttttg gcgactgaaa
     4621 cgatcttata agatgaataa actaatacgg ccaggataat agctcttata atccatttcc
     4681 atcgacgttt tcttttcctt tcttggagat agtcaattac caattgatta agcaaagctt
     4741 gtgaatccaa attagaatta gaagatgact cattattcat aaaaaactct tgaaaaattg
     4801 taaataatac ccattttatg gcatgtaaac tagaattaat accctaaaat aactagaaag
     4861 gtgttcctat gcgaatggac aaactaacat caaagtttca aatggcactt gcagatgccc
     4921 aatccctggc tttaggtaga gacaatggat ttatagaacc agaacattta atgaaggcct
     4981 tgcttgatca gcaaggtgga agttgcagac ctcttctcag taaagcgggt gttaatatac
     5041 cactattaag aactttaatt gaccaagctc tggataaact acctaaagta tcaggcaccg
     5101 gaggagatat tcacatatct aatgcattga atagactact taacctgact gacaaattat
     5161 cgcaacaaag aaaagataac tttatttcaa gcgagctttt tatattagca gcgataaatg
     5221 aggatagtaa ccttgcaaaa atacttaaac aagccggggg tgacaacaaa gcaattgaaa
     5281 aagcaattga tgagttaaga ggtggagaaa cggttaatga tccaaatgca gaggaacagc
     5341 gacaagccct tgaaaaatat acactggatc tgactgaacg tgccgagcaa ggaaaattgg
     5401 atccagttat tggacgagat gatgaaatca gacgcaccat ccaggtattg caaagaagaa
     5461 ctaaaaataa ccctgtatta ataggtgaac ctggtgttgg taaaacagcc atagtagaag
     5521 gtttggcaca acgaattatt aatggtgaag tacctgaagg attaaagaat aaacgcttac
     5581 tggcgctgga catgggtgcc ttgatcgcag gtgcaaaata tcgtggagag tttgaagaac
     5641 gcttaaaagg ggtgcttaat gatttggcaa aacaagaggg tcaaatcata ttatttattg
     5701 atgaattaca cactatggtt ggcgctggca aggctgaagg agctatggat gccgggaata
     5761 tgctcaagcc agcattagct agaggtgaat tgcattgcat tggggctacg acattagacg
     5821 aataccgaca atacattgaa aaggatgctg cccttgaacg acgttttcaa aaagtactgg
     5881 tcgatgaacc cagtgtggaa gacactatcg ctatattaag gggacttaag gaacgttatg
     5941 aagttcacca tggagttgaa attacagatc ccgctttggt tgctgcagca acgctatccc
     6001 atcgttacat cagcgaccgt caactacctg ataaagccat tgatttaatt gacgaggccg
     6061 ccagcttaat ccggatggaa attgattcaa aaccagaaag tatggataaa ctggaacgac
     6121 gcttgattca gctaaaaatc gagcgtgaag cattgaaaaa ggaaaatgac gaagcatcta
     6181 aaaaaagact tgttgattta caaaaaagta tcgatgaatt agagcaaaat tattcagact
     6241 tagaagaaat ttggaaagct gaaaaagcga ccatgcaagg gtccactcaa atcaaagagg
     6301 cacttgagca agccaaactg gaaatggaaa ctgcaaggag agcaggagat ttaagtcgta
     6361 tgtccgaatt acaatatgga cgtatacccg agttggaaaa aagattaagc caagtctctt
     6421 ccgttgatgc tatggaaaca aagttggtac gcaataaagt caccgaagat gagatcgcag
     6481 aagttgtttc aaaatggaca ggaatcccag tatctaaaat gatggaagga gaaaaggaaa
     6541 aactgctgaa aatggaagaa gctttgcaca gtagactcat tggccagaat gaagcggtgg
     6601 atgctgtttc taatgccata agacggtcac gcgcaggcct gtctgatcct aatcgtccaa
     6661 ttggttcatt tctttttctg gggccaactg gtgtgggaaa aactgaacta tgtaaagcac
     6721 ttgcttcttt tctttttgat acggaagagg cgatggtgcg cattgatatg tctgaattta
     6781 tggaaaaaca ctctgttgcg cgcttaatcg gagcgcctcc tggctatgta ggatatgagg
     6841 agggtggcta tttaactgaa gcagtcagac gcagacccta ttctgttatc ttacttgatg
     6901 aagtagaaaa agctcataca gatgtattta atattctgct tcaagtgatg gatgatggcc
     6961 gtctaacaga tggtcaggga cgtactgttg actttaggaa taccgttatt gttatgacct
     7021 ctaatctggg atctcaactc attcaggaaa tgagcagcaa atttaattac gatgagatta
     7081 aggctgcggt aatggattta gtcagccaac atttcaggcc tgaatttatc aacagaattg
     7141 atgaatcggt ggtattccat tccctgacaa aagaacaaat tgcaaaaatt gcagcaatcc
     7201 agattaacta tttacatcat cggctgaaac aacaaaatat cactattgaa gtcacaagtg
     7261 aggctttatc tcatttggct gaagcaggtt ttgacccggt ctatggtgcc cgccccctaa
     7321 aacgtactat tcagcaaaaa ttagagaatc cactagctca atcattattg actggaaaat
     7381 ttaaatccgg ggataccatt attgtatctt ataaagatgg agtaatggaa ttttacaagc
     7441 aatgagtatg cttatcattg gaaaataacg caggagtcct tatccaggac tccaaaatta
     7501 cggggcaatg tttcaagacc aagctgggta aacatatcaa acaaataaag acgtagtacg
     7561 agtctctgaa ttatcttcct gggctttgtt tactgtttga acttttttag ctaaagccaa
     7621 ttctgtttga agaataaatg tattcattga gccttttcca ttaaaaaaat caaaattaag
     7681 ctcaacttta ggattagctt caaaatatgt tttaagaagc ctgattgcat caagcatatc
     7741 cttatcagtc aactcattgg gatttatttc cacacccaga gcctggtgaa tgcaaaaccg
     7801 taattgctcc tttacgccct cgttttgtaa taacaaacaa taaccaccca aagcccgata
     7861 tcttgcaaga ctgctattac aatttttcat tgcttcaata aacgaaccac aatccatcgt
     7921 gctaaaattt tcctgaatat gtccggtata caacagatca ataatttttt cctgtaaaac
     7981 gttcgattca atttgagctt ttatttgctc ttccatcagt tccaaggata tggttttaaa
     8041 atcggaacag gtttttggca agaatttgcc taaatgagtc aaagcctctt caatttgacg
     8101 ttgctcttct tcaatttgtt ttaccaagga ttgaatgaaa tttatggcct tgaattgatt
     8161 taaaacaaca gggttacgtc ttttgtgttc atcaataatt tcttgtaaat aggtttcaaa
     8221 attcttgtct gctgcaaaat gaggatagct tttatagcgc ggcattttcc cctcatcctt
     8281 ccctacaatc aattgcatat tatcacgaaa tgtttccaaa gcagttacaa tcgttgttac
     8341 atcaagaatt ttcaagtcat ttattctata attcctctcc atcacctcag gtaaacctaa
     8401 agcgagtctt attgcttgaa ataatcgaca gtcttttaca ttagatggtg gcttactaaa
     8461 taaaaatgat gggtaaggaa tatatgaatt aaagttatca tactccttaa tcagtctaaa
     8521 atagcgatga agcaaagctc ctaataaaaa caggatggct tgcttatttt tttcttcatt
     8581 attttctttt aaaacttctc gcaattcatt acttaattgt ttaaaaacat ctctggcagt
     8641 ttctctatcc gttttgcaaa tcacaacctt atgggtccga gctaataaag tttccaaagt
     8701 atttctaatt atttcaccac tcgataccgc ttcagataca ggaggatcag taataatttt
     8761 atacctgcct agcaaggtag agaatattgt ctctaagtta cgagtattta cacttaatat
     8821 tttgaactcg ggcggagtaa aagtcatagt gtctcctgtc taaaacacgc tattaagtcc
     8881 taaagtacgc ataattcctt tataaatatc atagaatgaa gtttcctgtt tctccctgga
     8941 atgacgtaag gcatgaatat ctgcttgcac cgtcttacct aaacgtcgaa ttaagttgtc
     9001 ataaatgcgt tcattacgat tttgataggc aaatgaacaa tattgactta aagcctctaa
     9061 actggcatca aacaaagtgt cactcatctt ttgtggggaa ctcacttgca aatctctttg
     9121 aaatagctca accaaagtgc tattcattaa attcgagaca gtagagctat aatgtgacca
     9181 catcattaaa taaattccag ttagtacctt ttttttgaat tcgttaatat tttttggagt
     9241 tacagcagag tatgttgtga cacccttcag ataagcgatt aagtcatcgt cattctcgct
     9301 atccaggcta taagcaatca atttaatcgt atgagtatct tcaatagccg ctgtattggg
     9361 taatatcact gaaaaaaaac aaaaacagcc taaggatggt atgctgcgtt tctttaattg
     9421 atctgttaat ttcaatgcat tttgctgaaa ttcggcaaac tctagcatgg tttccctccc
     9481 acaagggccc acccgcaaga accgcagcga gtaatacgcc tttgatgatc aagactcttt
     9541 taatttttat atcaacctac acataatatt gcaactatta atttaactat ttgccaacaa
     9601 atatttcaaa tcgccaattt tatttggatg aagaaaataa atatattttt ccgtaggtta
     9661 ttctaaactc aagagatacc ttttactgtg atgatctttt tgtgttgggg ctttgcaaat
     9721 aatgggacat gacttttatt tcacctgttt ctcaatatca tgatatgttc tcaagacatt
     9781 tcagaataaa aatgaatcgt tacataaaaa gtttatctat agaatcgatc aattgttgat
     9841 gaattccatt aaagcctcta ttactcatta ccaatacagc atcgccttct cttactgttt
     9901 tgataatttc tttaactatc tctccattgt ttttgcaaat tttatatgga caagtccagg
     9961 agctaacaaa atcttttaaa ttaaaatcct gaggctccag gacgtaggca ccatcaacag
    10021 gagctaaagc atgagccatt tctgcggcat gtacacccgt tctcatagta tatgaagcaa
    10081 actccataac agcaaaaata cgttgatgtc gtccacttct ttttaaagca tcaatagttt
    10141 tagtaattgc ggttggatgg tgggcaaaat catcataaac ggttattcca tgtcgattgg
    10201 aacgaacttc aagccttcgt ttcactggag aaaactgttc t
//
LOCUS       DACTJE010000114        10215 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.114, whole genome shotgun sequence.
ACCESSION   DACTJE010000114 DACTJE010000000
VERSION     DACTJE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.114"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            58..1119
                     /gene="lem11"
                     /locus_tag="I9760_002141"
     CDS             58..1119
                     /gene="lem11"
                     /locus_tag="I9760_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem11"
                     /protein_id="HAT5043904.1"
                     /translation="MWYDTLLEYLSETICPSNKEKARLFISHLFKLLKENSDFGENFE
                     PEFIFGVMVALYNYSISHENQNVTLDEFGKYCMGLIMVFSKISDDLSIYCSDFTYILS
                     DKTILQELKIEGELAAIRKDYLKKKRTNPEVTPFVDQKDVANPFEIYKKIGELAKIDL
                     LKDLENHTLAYLNFSVKIQTEKIADMLNDLLNRVQNQQVIVSGLYQYLLPYKGKDDLF
                     DALIFSLKRELKKLSHNFSPKTQPSSISKINLVLVPSLKLDHQPRHNPEIVNICEKLE
                     NYVFRGSNKKSSFFSAKNHCGYTKEVNEIVQRILRGDLLTAEAIFHELAKINIESRKD
                     ELILLRNDLQSEKVCVRKL"
     gene            1282..2601
                     /locus_tag="I9760_002142"
     CDS             1282..2601
                     /locus_tag="I9760_002142"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAT5043905.1"
                     /translation="MKHQSNLSGRNVYVVDGSRTPFLKAKGVGPFCGSDLAVAAGTTL
                     LNRQPFSPDELDEVIIGSAMPGPDEANIARVVALRLGCGDKVPAFTVMRNCASGMQAL
                     DNAAIQIATGRSNLVLAGGTDAMSHAPLLFNEKMASWLANWFAAKSMGQKLALITKFK
                     PSYLAPVIALLRGLTDPIVGLNMGQTAEKVAYRFNITREQMDEFAAQSHLRIAQAYAE
                     NRMSEVAPIIDYKGCIYPQDDGIRADSTVEKLSKLKPFFDKKYGMVTPGNSSQITDGA
                     CMLLLASAEAVKKYALPVLGRIVDSQWAALDPSQMGLGPVHAATPILERHNLKPQDLD
                     CWEINEAFAAQVLGCLAAWNDEEYCKTQLGLKEAIGAPSLERLNRDGGAIALGHPIGA
                     SGARIVLHVLKSLEQRNESRGMAAICIGGGQGGAMYLERVTEVKGHE"
     gene            2594..4612
                     /locus_tag="I9760_002143"
     CDS             2594..4612
                     /locus_tag="I9760_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase"
                     /protein_id="HAT5043906.1"
                     /translation="MNNYKHWDLQRDKDAILWLGLNRRDAAVNSINEEVLDELNSLLH
                     EISHDKKAMGLIIYSLKEKGFIAGADVNAFSQFESSSQAVDFLQKGQTVFARLQALSI
                     PSVAMIDGFCMGGGYELALACTYRIATDDKDTRIGLPEVMLGIHPGWGGTVRLPQLIG
                     GFKALSQVILTGSAIPAAKAKSLGMIDEVVPVRQLRRAAVYFIKNKPPRHRASFIESL
                     TNSSLLRKAIAYLLRYNVAKRVRKEHYPAPYAVIDLWEKEGGVGERAYLKEVDSIEKL
                     VSQNDTAKNLIRAFLLRERLKGFAKDSHFKATHVHVIGAGVMGGDIAAWCALRGLQVT
                     LQDKSYEQIAPAIGRAHKLYSKKLRKPRLIQAAMDRLIADPHGHGVKRADVIIEAVFE
                     NLVVKHEIMKTVEKNAKKDAIIATNTSSIPLDEISSVMSKPERLVGIHFFNPVAKMDL
                     VEIVSSSSTTKKVQQSACAFVNQIGKLPLPVKSSPGFLINRVLMPYLMECVQLLDEGY
                     SAETIDEAALQFGMFMGPVELADTVGMDVCLAVAENLTSHFGGTIPQRLRDLVKEGKL
                     GRKTGEGFYKYKNGKVIKKKAPSHVDKTIADRLILRMVNESAACIREKVVEDSDLLDA
                     GMIFATGFAPFRGGPMNYANDFGKDKLETLFKTFESKYGERFKVDTSL"
     gene            4672..5406
                     /locus_tag="I9760_002144"
     CDS             4672..5406
                     /locus_tag="I9760_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAT5043907.1"
                     /translation="MEQKSKSIIRVWYLILLLSQYSFANVYVLPEAGNTVGEIQVAFS
                     EYGETIDEVGRRFNVGYYEMVRANPGVNARYPLAANTKLIIPSRFILPNVPRKGVVIN
                     LAEYRLYYFPPDDNVVMTYPVGIGKKGWETPLGLSRIISKEINPTWRPTVKLQKAAES
                     IGAPIPSEFPPGPHNPLGQHVLRLGWPAILIHGSNRVDGIGAKVSAGCIRMLPEDIEH
                     LYSLVSVGTPVRIINAPLQGQADFKQ"
     gene            complement(5443..6333)
                     /locus_tag="I9760_002145"
     CDS             complement(5443..6333)
                     /locus_tag="I9760_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAT5043908.1"
                     /translation="MQVIPFRYFILLAGTIVFLSLVALVINHFIYQFPGNNYFPPNTP
                     VMACIILLNYTGLILFFGKKNRVTCSGLELINFFIVMSIIALATNAVQLTPYPPIDKQ
                     IIKLEAQLGINMISILEWTNKYQHFKYLLAIIYDTLPYQMSILPLYVIVSGRFYILKD
                     YYFLLLCTTLMGFSYYYFFPTTAPASELQSSLFAPEQIATGLKFHQIHNYIHPTTIEG
                     GLIALPSFHTIWAVLCVYLIKDWFIPCLLLSIINVLLILSCVLLGWHYPIDILAAIIL
                     VGLSYYLLTVIKVEARYLDL"
     gene            complement(6344..6541)
                     /locus_tag="I9760_002146"
     CDS             complement(6344..6541)
                     /locus_tag="I9760_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HAT5043909.1"
                     /translation="MDIINLKFEEPLIIRISNTVVKILAFKTQENGNIKFGVEAPRSI
                     NIHREEVFHAIKQKETLSTAD"
     gene            6850..7188
                     /gene="rpsF"
                     /locus_tag="I9760_002147"
     CDS             6850..7188
                     /gene="rpsF"
                     /locus_tag="I9760_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAT5043910.1"
                     /translation="MRHYEIMFLVHPDQSEQVPGMVERYEGIITKHNGKIHRKEDLGR
                     RQLAYSINKVHKAHYILMNVECNLDALNEIKNAFKFNDAILRHLITVQKQAITTESVL
                     MKKEKETKVA"
     gene            7202..7429
                     /gene="rpsR"
                     /locus_tag="I9760_002148"
     CDS             7202..7429
                     /gene="rpsR"
                     /locus_tag="I9760_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAT5043911.1"
                     /translation="MSAYFRRKKMCRFSAEGGNEIDYKDINLLKNYITETGKIVPSRI
                     TGTQTRFQRQLAKAIKHARFIGLLPYCDSHR"
     gene            7431..8342
                     /locus_tag="I9760_002149"
     CDS             7431..8342
                     /locus_tag="I9760_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043912.1"
                     /translation="MNLAGNFILRQSKATLENKQYAIVCAVVLSIVPFASWLSVALMS
                     LITLRKGEKPGFEVMLPAMIVHSVPLMMFLPLEGALINTLVAYLPCYIAALTLRKTAS
                     WSNVFGAFLIQAFLVFLSIEILIPDFVSDQFNHFKVLLSQFQDYKQLVESSTDGLNNL
                     SLAQLFFGIQILSVIASALISLMFARTIQAKLFMPGGFKQEILEFRSGRFSFLMLIIV
                     SVASYFEVPVAISTLPLLLAYFFLSGFSLAYYILARKWQVRVVVLLFLLILLKPFFVL
                     FAYIVFGSLDSLFNFRLYLPARVREST"
     gene            8355..8804
                     /gene="rplI"
                     /locus_tag="I9760_002150"
     CDS             8355..8804
                     /gene="rplI"
                     /locus_tag="I9760_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAT5043913.1"
                     /translation="MEVILLEKVRNLGNLGDKVHVKSGYGRNYLIPQNKAVFATEQNI
                     ELFEKRRAELEKKAQQALANAEQRAAKLNDTTIVISAMASDEGKLYGSVGVNEIKDAL
                     IEKQIEISKREIVMPEGPLHSIGNYVVEVHVHSDVVANLQVEIIPAK"
     gene            9013..>10215
                     /gene="legC6"
                     /locus_tag="I9760_002151"
     CDS             9013..>10215
                     /gene="legC6"
                     /locus_tag="I9760_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC6"
                     /protein_id="HAT5043914.1"
                     /translation="MSHSKREQNPIARNLSDFRQVATLLNNEGLVVTEHNYKKVQALL
                     NRYKELATFIDQNQSKLNGKQIGELNQIRLIISPEERREIRKSVTRIENGIKNREERI
                     KEEYTRIEKQQAKAREVVQVLWPQLQKAAKKFINSGIANMEELLKEYKDLRNELYNHA
                     LNLPTQERKQKNQEMAELSRLVNIHESKLYEEINRARNVIEAAVTQAQKNVDSMSLLQ
                     MVRLMLPKLKDADILKIAQKAGVLSLQDTTTSEHGANLDKLVVREQPLEKQSALISIM
                     FKELALNGAQAGPLAKYLQAVQENAKDLISGKKTHFTLLYQTNEGEIGGFYFNIHELL
                     QNQHQAISKLVHEMYKRGVDQNSRVVMAFASPEQTASLMLANGENIKALTEAGNLQEL
                     TEQLLENAG"
BASE COUNT         3412 a         1647 c         2090 g         3066 t
ORIGIN      
        1 ttttttaaat gtatttatgt tatgacacta cctattaaag ggatttaaag gattattatg
       61 tggtatgaca ctctattgga atatttaagt gaaacaattt gtccatcaaa caaagaaaaa
      121 gccaggctat ttataagcca tttattcaaa ttattaaaag aaaactctga ttttggagaa
      181 aattttgaac cggagtttat ttttggtgtg atggtcgctc tttataacta ttcaataagt
      241 catgagaatc aaaacgtcac cttagatgaa tttgggaaat attgtatggg attaattatg
      301 gtattttcca aaatatcaga cgatctatcc atttattgtt ctgattttac atatatctta
      361 tcagataaga ccattttaca agaattaaaa attgaagggg aattagctgc gattagaaaa
      421 gattatttaa agaaaaaaag aacaaacccg gaggtcactc catttgttga tcaaaaagat
      481 gtagcaaatc ctttcgaaat atacaaaaag ataggtgaat tagccaagat agatctactg
      541 aaggatttgg aaaaccatac acttgcctat ctgaatttct cagtaaaaat ccagacagaa
      601 aaaatagccg acatgctaaa tgatttatta aatcgtgtac aaaatcagca agttatagtc
      661 tctggactgt accaatatct tctaccttat aaaggcaagg atgatttgtt cgatgccttg
      721 atattttcac tcaaaagaga attaaaaaaa ttaagtcata atttctctcc taaaacacaa
      781 ccttccagta tatcaaagat aaatctcgtg cttgttccaa gtttaaaact tgaccaccag
      841 cccaggcaca atccagaaat agtgaatatt tgtgaaaaat tggaaaacta tgtctttaga
      901 ggatccaata agaaaagttc atttttttca gcaaaaaatc attgtggcta caccaaagag
      961 gtaaatgaaa tagtgcaaag aatattgagg ggagatcttt taacagcaga agcaattttt
     1021 catgaattag ctaaaattaa tatagagagt cgtaaagatg aattgatttt gcttagaaat
     1081 gatttgcaaa gtgaaaaggt atgcgtaaga aagctttaac agagtaaggc ttaaagaata
     1141 aacacctcca gaatgattta cgtaatttaa tgtcatcaaa aaatcatctt aagatttcca
     1201 aaatatcaag aatactgcta atctttgaat ctgttgattt taatatagat tcttatgcag
     1261 aggccctaac aaggaggact aatgaaacac cagtcgaatt taagtggtcg taacgtttat
     1321 gttgtcgatg gaagtcgaac gccttttctt aaagcaaaag gagtaggtcc attctgtggt
     1381 tcagatttgg cagttgctgc aggaacaact ttgctaaatc gtcaaccatt ttctccggac
     1441 gagcttgatg aggtcattat tggtagcgct atgcctggac cagacgaggc caatatcgct
     1501 cgcgtggttg ctctgcgttt gggatgtggt gataaggtgc ctgcatttac tgtaatgaga
     1561 aattgtgcgt caggcatgca ggcattggat aatgccgcaa tccaaattgc tacagggcgc
     1621 agtaatttgg tccttgctgg tggaacagat gccatgagtc atgcaccgtt gttatttaat
     1681 gaaaaaatgg cttcctggct tgcaaactgg tttgcagcta aaagtatggg gcaaaagctg
     1741 gctttaatta caaagtttaa accttcttat ctggcaccgg taatcgcttt gctcagaggc
     1801 ctgactgatc ctatagtagg attgaatatg ggacaaacag ctgaaaaagt agcttatcga
     1861 tttaacatca caagagagca aatggatgaa ttcgcagctc aaagccattt aagaatagct
     1921 caagcttatg ctgaaaatag aatgtctgaa gttgccccaa ttatagatta taaaggttgt
     1981 atatatcctc aagatgatgg gattagggca gattccacag tggaaaaatt gtcaaaacta
     2041 aaaccttttt ttgacaaaaa atatggcatg gtcaccccag gcaacagttc acagataact
     2101 gatggtgcct gtatgttgct attggccagc gctgaggctg taaaaaaata tgctttaccg
     2161 gtattaggtc gaatagttga ttcacaatgg gctgctctag atccctctca aatgggtttg
     2221 ggtcctgttc acgcggcaac ccctatatta gaaagacata atttaaagcc acaagatttg
     2281 gattgttggg aaatcaatga ggcctttgcc gctcaagtct tagggtgttt ggctgcatgg
     2341 aacgatgaag aatactgcaa aacacagctt ggccttaagg aggcaattgg agcgccatcg
     2401 ctggagcgac tcaatcggga tggaggagca attgcgttag gtcatcctat tggtgccagt
     2461 ggggctcgta ttgtattgca cgttcttaaa tctttagagc aaagaaatga gtcgcgaggt
     2521 atggcagcaa tttgtatcgg aggtggtcag ggaggagcta tgtatcttga acgagttact
     2581 gaggtgaaag gacatgaata attataaaca ttgggattta caacgagata aagacgccat
     2641 tctgtggctt ggattaaata gaagggatgc tgctgttaat agcattaatg aagaagtcct
     2701 ggatgagtta aacagcttgc tgcatgaaat atcgcatgat aaaaaagcaa tgggattaat
     2761 tatttattcg ctcaaggaaa aagggtttat tgcgggtgct gatgttaacg ctttttcaca
     2821 atttgagagc tcttcacaag cggttgattt tttgcagaaa ggacaaactg tatttgctcg
     2881 gttgcaagct ttatcaattc caagtgtcgc aatgatcgat ggtttttgca tgggcggtgg
     2941 ttatgagtta gctttagcct gcacctaccg aatcgcaacg gatgacaaag acacacgtat
     3001 tggtttgcct gaagtaatgc tgggtattca tccagggtgg ggcggtactg tgcgtttgcc
     3061 tcagttaata ggtggtttta aagctttatc acaagtcata ttaaccggta gtgcaatacc
     3121 tgctgcaaaa gctaaaagcc tcggtatgat tgatgaggta gtacctgttc gtcagctaag
     3181 aagggctgct gtttatttca taaagaataa accaccacga cacagggcat catttattga
     3241 aagtttaacg aatagctctc tgctcagaaa agctatcgct tatttactgc gttataacgt
     3301 agccaagcga gttcgaaaag aacattatcc tgcaccatat gccgtgattg acctgtggga
     3361 aaaagaagga ggagtgggag agagggctta tcttaaagaa gtggactcta tagaaaaact
     3421 tgtatcccaa aatgataccg ccaagaatct tatcagagca tttttattgc gtgagcgatt
     3481 aaaagggttc gccaaagaca gccatttcaa agcgacccat gttcatgtga ttggagcagg
     3541 ggttatggga ggggatatcg ctgcctggtg tgctttgcga ggtcttcagg tcacattaca
     3601 ggataagtct tatgaacaaa ttgcccctgc aatagggagg gcacataaac tttatagcaa
     3661 aaaactgcgt aagcctcgat tgatccaggc cgcaatggat agattaattg cagatcccca
     3721 tgggcatggg gtaaaacgag cagatgtcat tattgaggct gttttcgaga atttggttgt
     3781 taagcatgaa ataatgaaaa ctgtggaaaa aaatgccaaa aaagatgcga tcatagcgac
     3841 aaacacctct agtatcccac ttgatgaaat cagcagtgta atgagtaaac cagaacgctt
     3901 ggtaggcatt catttcttta atcctgtagc taaaatggat ttggtagaaa tagtcagcag
     3961 ctcatctact acaaaaaaag ttcagcagag cgcttgcgcg ttcgtcaatc aaattggcaa
     4021 attgcctttg cctgtcaaat caagccctgg atttttaata aatagagtgc tcatgcctta
     4081 cctgatggag tgcgtacagt tattggatga aggatatagt gcggagacaa tagatgaggc
     4141 cgcattacag tttggtatgt ttatgggccc tgttgaactg gctgatacag taggaatgga
     4201 tgtttgtctt gctgtggctg aaaatctgac cagtcacttt ggtggtacta ttccacaaag
     4261 attacgtgat ttagtaaaag aaggtaagct aggcagaaaa actggagaag gtttttataa
     4321 atataaaaat ggaaaagtaa ttaagaaaaa ggctccatca catgtcgata aaaccattgc
     4381 agacagatta attttgcgca tggtgaatga atctgcagct tgtatacgtg agaaagtggt
     4441 tgaagattct gacttgttgg atgctggaat gatctttgcc acaggttttg ctccattccg
     4501 tggagggcct atgaattacg caaatgattt tggtaaagat aaactggaaa ctttatttaa
     4561 aacatttgaa tctaaatacg gcgaacgttt taaggtagat accagcttat aatttctctg
     4621 caaggtaaga cgttatggtt ttaccttgct ttttagagag aaaagcatca tatggagcaa
     4681 aaatcaaaat ctataataag agtttggtat ttaatacttt tactcagtca atattctttt
     4741 gcgaatgttt atgtattgcc tgaagccgga aataccgtcg gtgaaattca ggtcgcattt
     4801 tcagagtatg gagaaactat agatgaggtt ggccggcgtt ttaatgtcgg ttattatgaa
     4861 atggtacgtg ctaacccggg agtgaatgcc agatatccgc tagctgctaa cacaaaatta
     4921 attattccat ctcgttttat cctccccaac gttcccagaa aaggtgtagt gattaattta
     4981 gcagaatatc gattatatta ttttcctcca gatgacaatg ttgtcatgac ttatcctgtt
     5041 gggattggta aaaaaggatg ggagactcct ttgggattaa gcagaatcat ctccaaggaa
     5101 attaatccta cctggcgacc tacagtaaaa ttacaaaagg cagctgaaag cattggtgct
     5161 cctattccca gtgagttccc ccctggtcct cacaatcctt taggtcaaca tgtattgcgt
     5221 ttagggtggc cagctatctt aatacatggt tctaatcgag ttgatgggat tggggccaaa
     5281 gtcagtgcgg ggtgtattcg tatgcttcct gaggatattg agcatctata tagtttagtg
     5341 tctgtaggga cgccagtaag aattataaat gctcctctac aagggcaagc tgattttaag
     5401 caataactat tttcaggtat gggttttcag tcagattaat gcctataaat ccaaataacg
     5461 ggcctcaact ttaattacag ttaacagata ataagataat ccaacaagta ttatggccgc
     5521 caaaatgtca atcgggtaat gccatcccag cagtacacat gaaaggatta atagaacatt
     5581 aatgattgac agcaataggc agggtataaa ccagtctttg attaagtaga cgcacaaaac
     5641 tgcccagatt gtatgaaatg agggaagagc tattaaacca ccctcaatag ttgtggggtg
     5701 tatataatta tgaatttgat gaaatttaag tcctgtagct atttgttcag gggcgaataa
     5761 actgctttgt agttcacttg caggtgctgt agttgggaaa aagtagtaat aagaaaaccc
     5821 cattaatgtt gtacagagta ataggaaata ataatcttta aggatataaa atcgccctga
     5881 tacaattacg taaaggggta agatactcat ttgatatggt agggtatcgt agataatcgc
     5941 caataagtat ttaaaatgtt gatatttatt cgtccactcc agaatagata tcatattaat
     6001 acccagttgc gcctccagtt taataatttg cttgtctata ggaggatagg gagttaattg
     6061 aacagcgttt gtcgctaaag caatgattga catcacaata aaaaaattga tcaactctaa
     6121 tccagagcaa gtcactctat tctttttgcc aaaaaataaa atcaacccgg tataatttaa
     6181 caagataatg catgccataa ctggggtgtt aggtggaaaa taattattcc cggggaattg
     6241 atagataaaa tgattgataa ctaaagcaac tagcgataga aatactatag ttccagccaa
     6301 taaaataaag tatctgaaag gtataacttg catttatttg ttattagtca gctgtgctaa
     6361 gagtttcttt ttgtttaatt gcatggaaaa cctcttctct gtgaatattg attgacctgg
     6421 gtgcctcaac tccaaatttt atatttccat tttcctgtgt tttaaaagcc aaaattttaa
     6481 caacagtatt cgagatacga atgataagtg gttcttcaaa tttaagattt attatatcca
     6541 tttaaaaaaa acctcttttt tcataaatta ttcctaaggc caatattcac tgctgaacgg
     6601 taccttactt tggtgacggt tgtcactata aattgctgta aaatttataa aaaaatttat
     6661 ttaaagcagc tatgcgattt tgttgcctat aaaataggca aagttaagat acgcagcatt
     6721 aataaatatc ttattttaaa tcgcaatgag attgtaagga ctaaataaaa aagttataat
     6781 tggcagcttt ttaaaaccct tgccttacca gtactcaatg ctggaaggct ttatccataa
     6841 ggagaattca tgagacatta tgaaattatg tttctagtac atccagatca aagcgaacaa
     6901 gtacctggta tggttgagcg ctatgaaggg attattacca aacataacgg caaaatacat
     6961 cgtaaagaag atttaggtcg acgccagctg gcatattcaa taaataaggt tcataaagca
     7021 cattatatct taatgaacgt agaatgtaat cttgacgcac ttaatgaaat caagaatgca
     7081 ttcaagttta atgatgctat tttaagacac ttaatcactg ttcaaaaaca agcgattact
     7141 acagaatctg tcttaatgaa gaaagaaaaa gaaactaaag tcgcttaaga ggagaacatc
     7201 tatgtcagct tattttcgta gaaaaaaaat gtgccgcttt agtgctgaag gtggtaatga
     7261 aatagattat aaagacatca atctattaaa aaattatatt actgaaactg gtaaaattgt
     7321 accaagccgt attactggta ctcaaactcg tttccaaaga caattagcca aggcaattaa
     7381 acacgccaga tttattggtt tattgcctta ttgcgacagc catagataaa atgaatcttg
     7441 caggtaattt tatactcaga caaagtaaag caactcttga aaacaaacaa tatgctattg
     7501 tttgtgcggt tgttctttca atagtcccat tcgcctcttg gctatcggtg gctttaatga
     7561 gtttaattac tttgagaaaa ggtgaaaaac ctggatttga agtcatgtta cctgcgatga
     7621 ttgttcattc tgtgccttta atgatgtttt tacctctgga aggggcattg atcaatacat
     7681 tggttgctta tttgccttgc tatatcgctg ctttgacttt acgcaaaaca gccagctggt
     7741 caaatgtatt tggagctttt ctaattcaag cattccttgt atttctgtcc attgaaatat
     7801 tgatacctga cttcgtaagt gatcaattta atcattttaa agtgctattg tctcaatttc
     7861 aggattacaa acagttggta gaaagcagta cagatggatt gaataattta agtttggctc
     7921 agttattttt tggtatccag atattaagtg tgattgcttc agccttaatc tcactaatgt
     7981 ttgcccgaac tatacaagct aagcttttca tgcccggtgg ttttaaacag gaaatattag
     8041 aatttcgtag tggcagattt tcatttttga tgttaattat tgtgtctgtt gcctcttatt
     8101 ttgaggtacc agtggctatt agcactttac cactacttct tgcatatttc tttttatcgg
     8161 gtttttcttt ggcctattat attttagcca gaaagtggca agttagagtg gtggttcttt
     8221 tatttctatt gatcttgtta aaaccattct ttgtcttatt tgcctatatt gtatttggct
     8281 cgctagatag tttatttaat tttcgattat atctacctgc aagggtgaga gaatcaacgt
     8341 aaggggttat taagatggaa gtaatcttat tagaaaaagt acgtaatctg ggaaatctag
     8401 gtgataaagt acacgttaaa tcaggttatg gacgtaatta tttgatacca caaaataagg
     8461 ccgtctttgc aacagaacaa aatattgaat tatttgaaaa acgtcgtgct gaactagaga
     8521 aaaaagcgca acaggctttg gctaatgcag aacaacgtgc tgctaaattg aacgatacca
     8581 cgatagttat tagtgctatg gctagtgacg aaggtaaatt gtatggttca gtcggagtta
     8641 atgaaattaa agacgcattg atagaaaaac aaattgaaat cagcaagcgt gaaattgtta
     8701 tgcctgaagg ccctctacat tctataggta attatgttgt tgaagtacat gtacatagtg
     8761 atgtagtagc taatcttcaa gttgaaatta tccctgctaa ataatttgtt tgatatcgac
     8821 ctaaaaatat tcaaatcaac aatatttata taaattctta cttcaagttg tgcgtcacgt
     8881 cgcacaactt gttatctatt agtcaacatg cattaagatg ttgtagtttt gtaaaaaatt
     8941 gcccaattaa atgtgacaaa atagcattcc ttgattataa tgcgagcaca ctaaccatca
     9001 aggaatcatt ctatgtctca ttccaaaaga gaacaaaatc ctattgctcg gaatttaagt
     9061 gactttaggc aagttgcaac actattaaat aacgagggtc ttgttgttac tgaacataac
     9121 tataaaaaag tccaagcctt attaaataga tataaagagc tagcaacctt cattgatcaa
     9181 aaccaaagca aattaaatgg aaaacaaata ggtgaattaa atcaaattag attaattatt
     9241 tctccggaag agcggcgaga aatcagaaaa agtgttacac gtatcgaaaa tggaataaaa
     9301 aatagagaag aaagaataaa agaagaatat accagaatag aaaaacaaca ggctaaggca
     9361 agagaagtag ttcaagtttt atggccacag ttgcaaaaag cagccaaaaa atttataaat
     9421 tctggaatag caaacatgga agagctattg aaagaatata aagacttaag aaacgagtta
     9481 tataatcatg cgcttaatct tcctactcag gaaagaaagc aaaaaaatca agagatggct
     9541 gagttatcgc gtcttgttaa tatacatgaa agcaaattat acgaagaaat taatcgagcc
     9601 aggaatgtaa ttgaagctgc agtgactcaa gcacaaaaga atgtagacag tatgtcattg
     9661 ctgcaaatgg ttcgactcat gctgcctaaa ttgaaagacg cagatatatt aaaaattgct
     9721 caaaaagctg gagtactttc cttacaggat acaacaacct ctgaacatgg ggcaaattta
     9781 gataaattgg tcgtaagaga acagccatta gaaaaacaat ctgctctaat ttctataatg
     9841 ttcaaggaat tggctctcaa tggtgctcag gctggtcctc tggcaaaata cctccaggca
     9901 gttcaagaaa atgcaaagga tctaataagt ggtaaaaaaa ctcactttac tttgctttat
     9961 caaacaaatg agggtgaaat tggagggttt tattttaaca ttcatgaatt gttgcagaat
    10021 cagcatcaag caatttccaa gcttgtacat gaaatgtata agagaggagt agatcaaaac
    10081 agtcgagttg taatggcttt tgctagtcca gaacaaactg cgtctttgat gttagctaat
    10141 ggagaaaata ttaaagcatt aactgaagcc ggtaatttac aagagttaac tgagcaatta
    10201 ttagaaaatg ctggt
//
LOCUS       DACTJE010000115        10188 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.115, whole genome shotgun sequence.
ACCESSION   DACTJE010000115 DACTJE010000000
VERSION     DACTJE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.115"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..237)
                     /locus_tag="I9760_002152"
     CDS             complement(<1..237)
                     /locus_tag="I9760_002152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043915.1"
                     /translation="MNNNYKNALLFVQISTHFREIFRVARLMKKRGAYSPKVFFVAPY
                     EGWKQDFQQCQSEGIECICYFEKLIQNKSQIQNKS"
     gene            complement(276..1736)
                     /locus_tag="I9760_002153"
     CDS             complement(276..1736)
                     /locus_tag="I9760_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT5043916.1"
                     /translation="MNWILRVDDLSKVYRLGAGATQQGTLYEQLSKKVKKLLPGKKLS
                     SEDNEENFQKSSGRVVVSDAQTEGLPQGFFWALKNISFQITPGERIGLIGQNGSGKST
                     LLKILSRITAPTYGEFRYRGHLISLLEVGTGFHPELSGRENIYLNAAINGMTRQQINV
                     RFKEIVEFSELGNQIDTPVKRYSSGMYMRLAFSVAAFLESEILLIDEVLAVGDQDFQK
                     KCKEKMLEVANDGRSVIFVSHDMSAIESICKKTIEISHGEIVAIKDIEFGKRDKEINQ
                     IIDIRSSQVLDSNVEYQKDFNLRAKCEFKEELEKAAYKSAYVKLVSCQLMNDKGFLQE
                     KYSPDENINLEIKFKVLQANTRINVRVDLSTMDDIIIFSSIDNAYFQSKKIERVDGFY
                     KEIVTIPRNYLNEGQFSISIYIGDLEAGKEIVFCKDCLSLKVVDEQISFSEDSKLIRS
                     VPKPLLRSNFSWKIECCNDVRDEFETESLIEYEVSN"
     gene            complement(1733..2569)
                     /locus_tag="I9760_002154"
     CDS             complement(1733..2569)
                     /locus_tag="I9760_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT5043917.1"
                     /translation="MNESKVLKETVIKAGDIQPLKTYLKDLWHYRLLLWVFTARDIKV
                     RYSQTILGFGWILLSPLITVGVFTFVFGFMIKIPTDGLPYLLFYLVAIIPWYAFMAMI
                     NLTMGSVEGNAGLVSKIYFPRLLLGGTYTLSAGVDYLVGFCLIILCAILYHKLSFQLF
                     VLFPFLFAIQAMFAMGLGLMMAPFSTRYRDIKLFVPLALQLYYFANPILYPVSASPHW
                     LRLWYEFNPMSMIICSYRDALNGHWPSLEQFSFGFLAAIAIFIIGFNIFRKHEQSLVD
                     AI"
     gene            complement(2575..4074)
                     /locus_tag="I9760_002155"
     CDS             complement(2575..4074)
                     /locus_tag="I9760_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAT5043918.1"
                     /translation="MITIKELYIQKQENLKTALSKLDATAQGVLFLVDSDEHLIRTVT
                     DGDIRRLLLKGFTLDSTLEELSEHSSKALPVSATIQDAYHLMQEYELDHIPVIDESNR
                     PIRLIHRRELSSNILLSSPHIGEHEQQYVQEAFATNWVAPLGPNVDSFEKEVAEYINT
                     KSAVALSSGTAAIHLALVLLDVKPGDIVFASSFTFVATVNPILYQGATPVFIDSDLDT
                     WNMSPVALERALRESKAKNQMPKAVIIVNLYGQSANYDALCQLCNEYNVPIIEDAAES
                     LGATYNNKYSGTFGKLGVFSFNGNKIITTSGGGMLVSEDESLIQRARFLSTQAREPMP
                     HYEHTVVGYNYRMSNVLAGIGRGQLKVLEKRVKARRAIFDRYKQALESYPFIDMMPEI
                     NHGYSTRWLSTLVIKPMPNKIKPEEVIEQLKPFNIEARRTWKPMHRQPLFAGSKYYPH
                     DEQFSVSDYLFDHGICLPSGSNLTPRDIDRVIHCLNDIFKSKQNVVEVV"
     gene            complement(4085..4693)
                     /locus_tag="I9760_002156"
     CDS             complement(4085..4693)
                     /locus_tag="I9760_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAT5043919.1"
                     /translation="MHTNLKIIGCGGHCKVVIDALSLTQHSLQVSLCDSNKSLLGQEV
                     CGVLVDSTMDSLADYIGYVHVSIGNNQARKNVIDLMNSNANLFTIIHPAAIIAKSARL
                     GAGSFIAAQAILGPECQVGEGCIINHSAVVDHEVIVGSYSHIAPNSTLGGRVNVGERV
                     LIGAGAVVLPGVTIGDGAIIGAGSVVVKDVKENAVVKGVPAV"
     gene            complement(4683..5816)
                     /gene="neuC"
                     /locus_tag="I9760_002157"
     CDS             complement(4683..5816)
                     /gene="neuC"
                     /locus_tag="I9760_002157"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAT5043920.1"
                     /translation="MTRKIIYVTGTRADYGLMREVLKRLHQSEDIDLSICVTGMHLDA
                     LYGNTVNEIKADQFSICGIIPVDLANAQHSSMAKAIGHELLGFTEVFESETPDVVLLL
                     GDRGEMLAAAIAAIHLNIPVVHLHGGERSGTVDEMVRHAISKLSHYHFVATEASKQRL
                     IKMGEKEETIFQVGAPGLDEIMQYKTSTRDVFNQRYGFDPDKKICLLIYHPVVQEIDS
                     IKIQFQSVIQAALATNLQIICLEPNSDTGGHLIREVIQEYIDHSDVRIIKHLHRPEFI
                     DCLANSDVMLGNSSSGIIEAASFNLNVVNVGSRQNLRERSDNVIDVDVTYDAILTGLR
                     EALNKPKIKYSNCYGDGKTSERCYQLLKTIPLHSQILNKCNAY"
     gene            complement(5816..6838)
                     /gene="legI"
                     /locus_tag="I9760_002158"
     CDS             complement(5816..6838)
                     /gene="legI"
                     /locus_tag="I9760_002158"
                     /EC_number="2.5.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N'-diacetyllegionaminate synthase"
                     /protein_id="HAT5043921.1"
                     /translation="MNCFIIAEAGVNHNGDLQLAKELVYAAKESGADAVKFQTFKADT
                     LVNKTVEKAEYQKNNAPESATQYEMLKALELSEEDHYLLSELANSLGIEFMSTGFDEQ
                     SIDFLISLGVKRLKIPSGEITNVPYLQHCASKKLPLIISTGMCDLQEVRVAIDTVKPY
                     YGNSLSDYLVLLHCTSNYPAAYQDVNLKAMRTLADEFQLPVGYSDHTLGILVPTLAVG
                     MGACVIEKHFTMDKSLPGPDHLASMNPEEMKNLVQSIRDAETVLGSGEKKPSDNELPI
                     RALVRRSITLRRDLVKGAQISKEDLILLRPGTGIAPSEISNIVGARLSMNLSAGTTLL
                     WEHIEA"
     gene            complement(6835..7533)
                     /locus_tag="I9760_002159"
     CDS             complement(6835..7533)
                     /locus_tag="I9760_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAT5043922.1"
                     /translation="MRILAVIPARAGSKRLPGKNTRLLAGKPLIAHTIVAALQSSCCE
                     EIVVSTDSKQIADVAVQYGASVPWLRSEDLATDTSDVIHTVIDLLFKFQQMEVFFDSV
                     LLLQPTSPFRKPETIRHAVEIHQATGKSVVSVSPISLKPSWCRSIDSQGNLVKPELFQ
                     DLEIYCNENPIYKLNGSIYIATAKQIIENKSFYSEPTKPLLLNSISESIDIDTPIDWA
                     LTEKLMELNQEALV"
     gene            complement(7546..8187)
                     /gene="hisH"
                     /locus_tag="I9760_002160"
     CDS             complement(7546..8187)
                     /gene="hisH"
                     /locus_tag="I9760_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAT5043923.1"
                     /translation="MSSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLV
                     LPGVGAFEDGMKGLTTLNFIEPIKQFARSGKPFLGICLGMQMMLSRSTEFGQHEGLDL
                     IAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFM
                     AMPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLSIIQQFLQI"
     gene            complement(8184..8945)
                     /gene="wbuZ"
                     /locus_tag="I9760_002161"
     CDS             complement(8184..8945)
                     /gene="wbuZ"
                     /locus_tag="I9760_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl amidation-associated protein WbuZ"
                     /protein_id="HAT5043924.1"
                     /translation="MSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGA
                     DELIYMDTVASLYGRNNLSEIVKTTAENVFIPITVGGGIRSVDDAKQLLRCGADKVAI
                     NTAATKNPTLISDIARRFGSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWARN
                     GEEFGAGEILLTSIDQEGTRKGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCES
                     KADAVAMADVLHYKRIGLPEIRKYALDNGIHVRVL"
     gene            complement(8945..>10188)
                     /locus_tag="I9760_002162"
     CDS             complement(8945..>10188)
                     /locus_tag="I9760_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HAT5043925.1"
                     /translation="VISNQRPNSAVEYEHKKESKKHTIHFDDEGICDACRVAERKKST
                     INWEERDRQLRELCDRFRSKDGSYDCVVPGSGGKDSFYAAHILKYKYGMNPLTVTWAP
                     HMYTPWGWRNFQSWIHAGFDNHLFTPNGRVHRLLTRLAVENLFHPFQPFMIGQKAYAP
                     KMALLHKIKLVVYGENEAEYGNPIGDTESAKRDWKYFTADDKSKIFLGGTSVQELKSD
                     FGLNDNDLDAYLPADPQQIEEQQVEVHYLGYYLKWHPQSCYYYSVEHGGFEASPERTP
                     GTYSKYNSIDDKIDDFHYYTTLTKFGIGRATYDASQEIRSGDITREEGVALVKRFDQE
                     FPERFAEEIFKYLSINPKEFPIASQMFEQPIMDRAYFMALADTFRSPHLWKKEGEQWK
                     LRHQVTNLEKTKAEYLDLETV"
BASE COUNT         3126 a         2231 c         1633 g         3198 t
ORIGIN      
        1 actcttattt tgaatttgac tcttattttg aattaatttt tcaaaataac aaatacattc
       61 aatcccttct gactgacact gctgaaaatc ctgcttccaa ccttcataag gggcgacaaa
      121 aaatactttt ggggaataag cacctcgttt tttcatcaat cgagcaacgc gaaatatttc
      181 tcgaaaatgc gttgatattt gtacaaataa taaagcattt ttatagttat tattcatttt
      241 ttgcagtaac cattggcact tcagttttaa taaaattaat tagaaacttc gtactctata
      301 agtgactctg tttcaaattc atcccttaca tcattacaac attcaatttt ccaagaaaaa
      361 ttagacctca ataaaggttt cggtacagaa cgtattaact tggaatcttc actaaaggaa
      421 atttgctcat caacaacctt caaagataaa caatccttgc aaaatacaat ttctttgcct
      481 gcttctaaat ctccaatata aattgaaatt gaaaactgac cttcattcaa ataattacga
      541 ggtatggtta caatttcttt gtaaaatccg tctactcttt cgattttctt agattggaaa
      601 tatgcattat cgatactcga aaaaattatt atgtcatcca tagtggataa atcgactctt
      661 acattaattc ttgtatttgc ttggagaacc ttaaatttaa tttctaaatt aatattttca
      721 tcgggtgaat atttttcttg aagaaatcct ttatcattca ttaattgaca agataccagt
      781 ttcacataag cacttttata tgcagctttt tctagctctt ctttaaattc acatttcgct
      841 cttaaattaa aatctttttg atactcaaca ttgctatcaa gaacttgact gcttctaata
      901 tcaataattt gatttatttc cttatccctt tttccaaact caatatcctt aattgcaact
      961 atttcaccat gagagatctc tattgttttt ttacaaatcg attcaatagc actcatatca
     1021 tggctaacaa aaataactga gcgtccatca tttgctactt ccagcatttt ttctttgcat
     1081 tttttttgaa aatcttgatc tcctactgct aacacttcat caattaataa aatctctgat
     1141 tcaagaaagg cagcaacgga aaacgctagc cgcatataca taccggaaga atatctcttt
     1201 actggagtat caatttgatt acctaattca gagaactcaa caatttcctt aaagcgcaca
     1261 ttaatttgtt gccttgtcat cccattgata gcagcattca aataaatatt ttctcttcct
     1321 gacagttcag gatgaaaacc cgtaccgact tcaagtaaac tgattaaatg ccctctgtat
     1381 cgaaactcac cataagtggg tgcagtaatt ctggacaaga tttttaataa agtacttttc
     1441 cctgatccat tctgtcctat tagacctata cgttcacctg gagtaatttg aaaagaaatg
     1501 ttctttaaag cccagaaaaa accttgagga agaccttcag tttgtgcatc agaaacaacc
     1561 actcttccag aggatttctg aaaattctct tcattatcct ccgaacttaa tttcttacca
     1621 ggtaataatt tttttacctt ttttgataat tgttcatata aagtaccttg ttgggtggca
     1681 cctgcaccca atctatatac tttgcttaaa tcatctactc ttaaaatcca gttcatattg
     1741 catctactaa agattgttca tgttttctaa agatattaaa cccaatgata aatatcgcaa
     1801 tcgctgctaa aaatccaaaa ctaaactgtt ccaaactggg ccaatgccca tttagcgcat
     1861 ccctataact acaaatgatc atactcatag gattaaattc ataccaaagc cgtaaccaat
     1921 gtggtgaagc tgaaaccgga taaagtatag gatttgcaaa ataatataac tgcaatgcca
     1981 atgggacaaa taattttata tctctatatc ttgtactaaa aggagccatc atcaacccta
     2041 atcccatagc aaacatagcc tgaattgcaa ataaaaaagg aaatagaacg aataactgaa
     2101 aactgagttt atgatataaa atggcacaaa ggataataag gcagaaacca actaaataat
     2161 ctacaccagc agatagggta tatgtcccac caagcaataa tctagggaaa tatattttac
     2221 tcactagccc tgcgttaccc tctactgacc ccatagttaa attgatcatt gccataaaag
     2281 cataccaggg aatgattgca acaagataaa ataaaaggta aggcaaacca tctgtaggga
     2341 ttttaatcat aaatccaaac acaaaggtaa acacgccaac tgtaattaat ggggacaaaa
     2401 gtatccaacc aaaacctaat atcgtttgcg aatatctgac cttaatgtct ctggcggtaa
     2461 atacccataa caacagtcta tagtgccata aatctttcaa ataagttttc agaggctgga
     2521 tatcccctgc tttgattact gtttccttta agactttgct ttcattcata gtcgttatac
     2581 aacctctaca acattttgtt tagatttgaa aatatcattc aaacaatgaa tgactctatc
     2641 aatatccctg ggagttaaat ttgacccgga aggaagacaa ataccatgat caaacaagta
     2701 atctgaaacg ctgaattgtt catcatgagg atagtattta gagcctgcaa ataaaggctg
     2761 tctatgcata ggcttccagg ttcttcttgc ttcaatgtta aatggcttta attgctcaat
     2821 gacttcttcc ggttttatct tgtttggcat tggtttgata accaatgtcg ataaccaacg
     2881 agtactgtat ccatggttta tctctggcat catgtcaata aatgggtaag attccaaggc
     2941 ttgcttatat cgatcaaaaa tagcccgtct tgccttaacc cgtttttcca atactttcag
     3001 ctgccctctg cctataccag caagcacatt actcattctg taattatacc caaccacagt
     3061 atgttcataa tggggcattg gttcgcgagc ctgagttgat aaaaatcttg ctctttgtat
     3121 gagactctca tcttcagaca ccaacatgcc gccaccagac gtggtaatga ttttgttgcc
     3181 attaaatgaa aacacgccta acttgccgaa agtcccgctg tacttgttat tataggtggc
     3241 tcccaaagac tctgccgcat cttctataat tggcacattg tattcattgc ataattgaca
     3301 taaagcgtca taattagcac tttgtccata taaattaaca ataatcacag cttttggcat
     3361 ttgatttttt gctttacttt ctcttaatgc ccgctcaaga gcaactggag acatattcca
     3421 ggtgtccaaa tcagaatcaa taaataccgg agtcgcacct tggtataaga tagggttcac
     3481 tgtggctaca aaagtaaagc tggatgcgaa cacaatatct cctggtttca catcgagtaa
     3541 tacgagtgcc aaatgaattg cagctgttcc tgagcttaaa gccacagctg attttgtatt
     3601 aatgtattcc gccacttctt tttcaaaaga atccacattg gggcctaaag gggctaccca
     3661 gttggtggca aaagcttcct gtacgtattg ctgctcatgc tcaccgatat gaggcgagga
     3721 aagcaggata ttggaagaga gttctctccg gtggatcaag cgtatggggc gattactctc
     3781 atcgataacg ggaatatgat ctaattcata ttcctgcatt aaatgataag catcctgaat
     3841 cgtagcagag acaggtaaag cttttgatga atgctctgat aattcctcta aagtagaatc
     3901 taatgtaaat ccttttaata gcaatcttcg gatgtcacca tctgtaactg tccttattaa
     3961 atgttcatca ctatctacca gaaataacac accttgagct gtagcatcca gcttgctcaa
     4021 agcagttttc agattttctt gtttttgtat atataactct ttgatagtaa tcattttatt
     4081 ccttttaaac tgcgggtacg cctttcacaa cagcattttc ttttacatcc ttcacaacaa
     4141 ctgagccagc tccaattatt gctccatcac ctatagtcac accaggcaaa acaactgctc
     4201 cagctccaat caacactcgc tcaccgacat tgactcgccc acctaaggtg ctattaggcg
     4261 caatatgcga atacgagcca actatcactt cgtgatccac aacagcgcta tgattgatga
     4321 tacatccctc acccacctga cattcagggc cgagtattgc ctgtgctgcg ataaaagaac
     4381 ctgcaccgag cctcgctgat ttcgcaataa tggcagccgg atgaataatt gtaaacaagt
     4441 ttgcattgga attcatcaaa tctataacat tctttctggc ctgattattt ccaatgctaa
     4501 cgtgcacata accaatatag tcagctaaac tgtccatagt tgaatcaacc aatactccac
     4561 acacctcctg acccagcaaa ctcttattgc tatcacaaag tgaaacttgt aaagagtgct
     4621 gagtcagtga taaagcatca attaccacct tacagtgtcc gccacatcct attattttca
     4681 agttagtatg cattgcattt attcaatatt tgtgaatgca aagggatagt ttttaacaat
     4741 tgataacacc tttcacttgt ttttccatcc ccgtaacaat tagagtattt tatcttaggt
     4801 ttatttagcg cttctcttag accagtcaaa atagcatcat aagtaacatc aacatcaatg
     4861 acattgtcgc ttcgttctct taaattttgc ctgcttccaa cattaactac gttcaggtta
     4921 aatgaggctg cctctatgat gccactactg gaatttccca acatcacatc agaatttgca
     4981 aggcaatcaa taaattccgg acgatgtaag tgcttgataa ttctaacatc agaatgatca
     5041 atatattcct gaatcacttc tcgaattaaa tgaccgcccg tatcggaatt cggctcaagg
     5101 caaatgatct gtaaatttgt ggctaatgct gcctgaatca cactttgaaa ttgaatttta
     5161 atcgagtcaa tttcttgaac aaccgggtga tagattaata aacagatttt tttgtcagga
     5221 tcaaatccat aacgttgatt aaacacatca cgtgtagacg ttttatactg catgatttca
     5281 tccaagcctg gagcaccaac ctgaaaaatg gtttcttctt tctcacccat tttaatcaat
     5341 cgttgtttgg atgcctctgt tgcgacaaaa tgataatgag ataatttgga aatcgcatgc
     5401 cttaccattt catcaacggt tccagagcgc tctcctccgt gcagatgtac aaccgggata
     5461 tttaaatgta tcgctgctat ggccgcagca agcatttcac ctcgatctcc cagcaataaa
     5521 acgacatcag gagtttcact ttcgaatacc tcggtgaatc ccaaaagctc atggccgata
     5581 gcttttgcca tagaactatg ctgggcattg gcaagatcaa caggaataat gccgcatatt
     5641 gagaactgat ctgctttaat ttcgttaact gtattcccat acaaagcatc aagatgcata
     5701 ccagtgacac aaatcgataa gtcaatgtct tctgattggt gtaatctttt tagtacttct
     5761 cgcatcagtc cataatcggc acgagtacct gtaacataaa ttatttttct ggtcattacg
     5821 cttcaatatg ttcccataac aaagtagtac ccgcggacaa attcatactt aatcgagcgc
     5881 ctacaatatt tgatatttca gagggcgcta tcccagtacc aggtcttagc aagatcaaat
     5941 cttctttact gatttgtgct cctttcacca aatcacgtct taaagtaata cttcttctaa
     6001 ccagagctct tatcggcagc tcattatccg acggtttttt ttcaccactt cccaaaacag
     6061 tttcagcgtc tctaatggat tgtactaaat tcttcatttc ctctggattc atagaagcca
     6121 gatgatctgg accaggaaga gatttatcca ttgtaaagtg cttttcgata acacacgccc
     6181 ccattcctac tgctaatgtt ggcaccaaaa tacctaaagt atgatcggaa tagcccacag
     6241 gcaattgaaa ttcgtcagct aatgtccgca tggctttcaa attcacgtcc tgataagctg
     6301 ccggataatt agaggtacaa tgcaacaaaa ccagataatc ggataaagag tttccatagt
     6361 agggttttac tgtatctatg gctactctta cttcttgtag atcgcacatc cctgttgaga
     6421 tgattaatgg taacttctta ctggcacaat gttgtaaata aggtacatta gtaatttcgc
     6481 cagaagggat ttttaatcgc tttaccccta aagaaataag aaaatcgatg ctttgttcat
     6541 caaaaccagt tgacataaat tcaatgccga gtgaattggc taattcactt agtaaataat
     6601 ggtcttcttc tgaaagctcc agtgccttga gcatctcata ctgggtagcg gattctgggg
     6661 cattattttt ttggtattcc gctttttcta ctgttttatt gaccaaggtg tccgctttga
     6721 aagtctgaaa ctttactgca tcagctcctg actctttagc tgcataaact aattctttag
     6781 ccagttgaag atcaccatta tggtttactc ctgcttcagc aattataaaa caattcatac
     6841 tagagcctct tggtttaatt ccattaattt ttctgttaaa gcccaatcga ttggcgtatc
     6901 gatgtcgatg gattcactaa tgctatttaa caataaaggt ttggttggtt cgctataaaa
     6961 actcttattt tcgataattt gtttagctgt tgcaatataa attgacccat ttaatttata
     7021 aattggattc tcgttgcaat aaatttccag atcctgaaaa agctctggct taaccaaatt
     7081 gccttggcta tcaatgcttc tacaccaaga aggctttaaa gagatgggac taactgaaac
     7141 gacacttttt cccgttgctt gatgtatttc aacagcatgt cttatggttt ctggcttcct
     7201 gaatggagaa gttggttgta aaagcaatac actgtcaaaa aaaacctcca tttgctgaaa
     7261 cttaaacaga aggtcaataa cagtatgaat cacatccgaa gtatccgtgg ctaaatcttc
     7321 cgatcttagc cagggtactg aagccccata ttgaacggcg acgtctgcta tttgtttact
     7381 atcggtcgaa acaacgattt cttcacaaca agacgactgc aaggcagcaa caatagtatg
     7441 tgcaattaat ggctttccgg caagtaatct ggtattttta ccgggcagcc tctttgagcc
     7501 agcccttgcc gggattactg ccaatattct catttttaat tcaccctaaa tctgcaaaaa
     7561 ctgttgaatg atgcttaaac ctacttcccc gcttttttca ggatgaaatt ggcatccgta
     7621 catattttca tcttttatta ctgcgctaat agcctgacca tcatataaag tatcagccag
     7681 acgcttttta ggattactag gcatggccat aaaagaatga acaaaatata ctgaagaatt
     7741 taatggtata tttttcagga tagtatgaca ccaatcctcg ccttctgagg tggaaaccag
     7801 ttcattccag cctatatgag gtattttatg caattgacca tcaactccat gtgaaggaac
     7861 gctaacaacc tcgccagcta ttagatccag gccttcatgt tgtccgaatt cagtacttct
     7921 tgagagcatc atctgcatgc caagacatat tcccagaaaa ggttttccac tccgcgcaaa
     7981 ttgctttatg ggttctataa aatttaatgt agttaatcct ttcatcccat cttcaaaagc
     8041 gcctactcct ggcaaaacca accgatcggc actcataatt tcctcgggtg tgctcactaa
     8101 attgacagaa gcatcaaaat attgaaaagc tcgagccaca cttaataaat tgcctacccc
     8161 ataatctaca attgaaactg aactcataag actctcacat gaatgccatt atctaaagca
     8221 tactttctga tttcaggtaa acctattctt ttataatgca aaacatccgc catagcgact
     8281 gcatcagcct tagactcaca aaccacttca gttaaatctt ccagcttacc cataccccca
     8341 cttgcaatca ccgggataga aaccatggaa gacacctgtt tcactaattc cagatcaaac
     8401 cccttacgcg taccttcctg atcaatagag gtcagaagaa tttcacctgc accaaattct
     8461 tcgccattcc tagcccaatc aacaacatcc attccggtat gttcacgtcc attgtctgtc
     8521 atgacctccc atcttccatt cacagtgcgt tttgcttcga tagataaaac aacacattga
     8581 gacccaaatc ttctggcaat atcagatatt agagtagggt ttttagttgc cgctgtatta
     8641 atggctacct tatcggcacc gcaacgcaat aattgcttgg catcatcaac actgcgaatt
     8701 ccgccaccta cagtaattgg gataaataca ttttcagcag tagtctttac aatctctgac
     8761 aaattattac gcccatacaa agatgcaact gtatccatat aaatcaattc atctgcacct
     8821 tgtgcataat atgccatagc atattcattg ggatttccta ctactcttaa tccctccaga
     8881 tgcactcctt tgattaggtt aggtccttta atatctagtc gtgcaattag acggacatta
     8941 ctcatcaaac agtctccaaa tccagatatt cagctttagt tttttcaaga tttgttactt
     9001 gatgtctaag tttccactgt tctccctctt ttttccacaa gtgaggggat ctgaaggtat
     9061 ccgccaaagc cataaaatag gctctgtcca taatcggttg ctcgaacatt tgcgaagcaa
     9121 tgggaaactc tttggggtta atactcaagt atttaaaaat ttcttcagca aaacgctctg
     9181 gaaactcttg atcaaaacgc ttcaccagag caacaccttc ttcgcgagta atatcccccg
     9241 aacgtatttc ctgggaagca tcgtatgttg ctcttccaat accgaatttg gttagcgtgg
     9301 tgtagtaatg gaaatcatca attttgtcat cgatactatt gtatttactg taggttcctg
     9361 gagttcgttc aggagaagct tcaaaaccgc catgttccac agaataataa taacaacttt
     9421 gaggatgcca cttcagataa taacccaagt aatgaacctc tacctgctgc tcttcgattt
     9481 gctgtggatc agcaggtagg taagcatcca aatcattatc atttaaaccg aaatcgcttt
     9541 ttaactcttg aacagatgta ccgcctaaga agattttgct tttatcgtct gcggtaaagt
     9601 atttccagtc tcttttcgca gattctgtat cacctattgg attaccatat tccgcttcat
     9661 tttcaccata aacaaccaat ttaattttat gcaacaaagc cattttagga gcataagctt
     9721 tttgaccaat cataaagggt tgaaatggat ggaatagatt ctcaactgca agtcgtgtca
     9781 atagcctgtg aactctgcca tttggagtaa acagatgatt atcaaatccg gcatgtatcc
     9841 aggattgaaa attcctccaa ccccatggcg tatacatatg gggagcccaa gtcacagtta
     9901 agggattcat tccgtattta tattttaaaa tgtgggcagc ataaaaacta tcctttcctc
     9961 cagaaccagg aacaacgcaa tcataagaac catccttgct tctaaatcga tcacaaagct
    10021 ctctcaattg cctgtctcgc tcttcccaat taatagtaga ttttttgcgt tcggcaactc
    10081 gacaggcatc gcaaatgcct tcgtcatcaa aatggatggt atgttttttt gattctttct
    10141 tgtgctcata ttctacagca gaattaggcc tttgatttga aataacac
//
LOCUS       DACTJE010000116        10158 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.116, whole genome shotgun sequence.
ACCESSION   DACTJE010000116 DACTJE010000000
VERSION     DACTJE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.116"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(125..1252)
                     /locus_tag="I9760_002163"
     CDS             complement(125..1252)
                     /locus_tag="I9760_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAT5043926.1"
                     /translation="MDMKKYILSLSLLTVINSSAIATTQEGEEAYNKGYYEKAFILLS
                     PEADKGDAKAQYLLGKMYYNAQGVTYNPEKTEQLLLASANQGNVDAQVLLAGFYWYLN
                     TPEGYKKAFEWYQKAADQNNADGQYGLGYMYDTGTGVPQNSDTAMVWYKKAAEQGNSN
                     AALAIGYNYDTGTGVKKDKTQALNWYAKAADLGNASAQYNLGLMYEQGDGVPKDYQKA
                     AEYFEKAANQGHAKSQLELGYLYDSGKLGKSDLQKAAFWYQKSADLGNANAQFNLADM
                     YFYGDGVGKSLEQSVYWMQKAAEQGYGKAQNQLGIYYRDGIGVAADPVKAYAWFTAAK
                     NNGFEKAASNASDLEKSMNPEDLSKARILGQQYTDNYKAKK"
     gene            complement(1506..2729)
                     /locus_tag="I9760_002164"
     CDS             complement(1506..2729)
                     /locus_tag="I9760_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2223 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043927.1"
                     /translation="MVDEKNYFLKIDFARQRIIDLPAGEFKYYLYILLESIKSQNVPS
                     RHEFLAVLLKLLDTLYKLKELKTSNFLDNQCPSSQLLKELMLHLCELQQVAKTHHFTA
                     QVMVVLLHIGGIISAVALSVVGGLLGGILGFVNGLCEYKPVIGFLVGTFTGTVLGGLF
                     GFRAPKKLFKNEIQRQIKFGLDGLGESLDNIKQEYSSSMFGPNKIKPFSFYLGQVETE
                     IKQLFNDDSEYEEFLNHKIDYEINSFLASFIGESLLHGCVGHHAYIKINIKNREYLIE
                     FTPQPTDTKEAPAQSETRTVSGKKIVEMLAYHKKLQETNPPTASFILTKMKPGDNDCF
                     SYVNKILIGTNQQGATIKRFANMGLIGSLLGMGIETLSPFKPDFFQTTSSVRTHKEKM
                     PGHSNDLSMNSLLPQ"
     gene            3369..5150
                     /gene="ppgA"
                     /locus_tag="I9760_002165"
     CDS             3369..5150
                     /gene="ppgA"
                     /locus_tag="I9760_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PpgA"
                     /protein_id="HAT5043928.1"
                     /translation="MKEPRELNRQPNPEHMAQLIKDTPKDVWFHLAKYLPDHELLNLM
                     QVDKRLNRFFKEGTPPKEIIKKIEHAPLKVVSSGVNTFILTQYHGKPLLLACGQNKDG
                     QLGCGDTKIRTALTPVNIPESFASIESVQISLFHAVLWGRDKDNKPILATCGLNDKGQ
                     LGCGDYKSRALFTQVKLAEDITSIESVEINIFHSVILGHDKHNRPVLASCGSNHLGKL
                     GCGDIKDKTTFTRMKLPTEIVSIDTLQMNWHTIVSGRDKNNKPVLAACGWNDRGQLGC
                     GDKKERALLTPVKLPENIVSIQSAQIGTSRTTVLGRDKDNKPILTYCGWNERNQMGWS
                     NTEDKTALTPAKLPENIVSIELMQISTSRTVILGRDKNNKPILAACGLNNHGQLGCGD
                     IKTRTTLYPIKFPKDIVSIESVQISDNHTVILGRDKHKNPIIAACGLNNYGQLGCGDT
                     KNRMLLTPIKLPKEIVCIDSVTVKYSSLFVSGRDKNHRPILAACGDNRYGQLGVPSKK
                     LLTELTIVPTPILTLPATPKREASSRKEATEKALVFATHGSREQKPRKSNRILTAIIA
                     SFGRFFTGAHPHTPDSDKESPSPKRSA"
     gene            complement(5345..6874)
                     /locus_tag="I9760_002166"
     CDS             complement(5345..6874)
                     /locus_tag="I9760_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HAT5043929.1"
                     /translation="MNWKRFLIAAMTKKKYQFFLNDTKHPKEARERLWNSEIVPLLKK
                     SSYWQELLKDQSSIVLNDFGITEYEDYQEGLLAAQHSTIQPFNGEELIFWSETSGTSG
                     VRKFFPITDSFQKQFQRTMAPYIYSLTQRFPGFFKEKMLYLVAVDTHKTTPAGIPTGW
                     ISNFNYRHLPSFIKKFYAMPDEVFASHEAYNQWGPIYGLASDLSAVFAVTPMVIDAFY
                     QRCIDGFKDYLPYLLGDKLLPSHLPPLKITRKRRRHLQALAKVDHLSFKQLWPSLEMA
                     GCWTSGLCEYPAQQLQKLLGSEIKLVDGTYSATEGWLTVPVETNSVGGILHPGAHIVE
                     FIPEGAAIEKENLLQCWELEVGKKYEVFLTTAMGFVRYRLKDIVKCTGYLNSSPKLEF
                     CYKTQLLKLETCSITGKELQSAVQATLINLEPYWYFARNSVGNRIVLVTDDEAEISEL
                     PLKKLHEQLIHISPTYAHSVETEEVLPVTLLQLPKEKLLAGMHGQTKPKFISQEVIAE
                     R"
     gene            complement(6881..8572)
                     /locus_tag="I9760_002167"
     CDS             complement(6881..8572)
                     /locus_tag="I9760_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT5043930.1"
                     /translation="MTSYENLIQLSEFFESCLGNPLENNSPVNFQQILLADEQEVLAS
                     SAIEFIKQWGYLDYTIPHYLGGKLSSLDELYTLTRLLARRDITMAIMFGLAFFAALPI
                     WIAGNSKQKKHLSESLRQGKIGALALTEEEHGTDLTSNKMNAKPIEDGWEISGRKWCV
                     NFATLSEYAVVLCRTNEKGGLLGFSLFYIDKKLAEKGISPTPKLPTHGVRGLDISGFS
                     FDKVKVSKDALIGKEQRGLEITYKVFQISRTLCACLAIGGADTALRLALSYSLERQLY
                     GKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTVIPKKMSLWSAIIKFLIPHIGE
                     DIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGM
                     RGKHQAEHSEKIEKIFNVRLSCPPFKEDRLGLFTHEEDDVLAGIMRLNSPAINPLITT
                     IRHEIEKLDQEVIHLHDQKLFDPRSLTVFRLAEKYCWIFAASCCLQFWHYNQEILSDD
                     LQNTDWLDLAMQLILKRLNSCSMIDSLLQEAMSERLYSFYQEKQLFSIMPIHIAG"
     gene            complement(8581..10002)
                     /locus_tag="I9760_002168"
     CDS             complement(8581..10002)
                     /locus_tag="I9760_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl CoA carboxylase subunit alpha"
                     /protein_id="HAT5043931.1"
                     /translation="MSSQHTDKTLIKWIDNLIDEQSFLPIGSEYIPDNNLHSLPGAEV
                     ITGLAKVNQRPVAIYAHDSSVNRGYVTRQGARKIIHLMDKAEKLRIPIVAFLASPGVS
                     LEENLLSGDEYTQIIARNIRLSGIIPQFAVILAPTLGAPAYSAVLMDLLFFNKHRSYL
                     MVTSPTVVQQAISEKVTMGELGSATLHATTTGIADFVDDSVTAQINHVKAMLGFFPLH
                     TNERPPVHPIMEPNHPIPDIPANPRMPFNMLNLIQAVVDNSNVIQYKRTYGQAMICAF
                     VHIHGFAVGVVANQSIRYSGAIDSDAAQKAAKFIRICDAYSIPILTFIDVPGFMPGKR
                     EEQKGLLQHGARFCVAMQTRVPRLSVIVRKCYGAAAFLMMQTKSQNGDLVLALETANL
                     GVMGKETTNKVQAMDGQPVQESSQSQLTEKSQIDDSLMEAYSLGLIDEIIQAAQIRSR
                     LASHLEFLYRSMETMPPAKHSIV"
BASE COUNT         3171 a         2202 c         1799 g         2986 t
ORIGIN      
        1 gaaattaatt ttatattttt ctatttttgg gctgccgaac ccgagtgtat gcgagaattc
       61 tctcgcacac tcccatagcc cacaaatgca gtatccctag ctcttgaaaa caggtaacag
      121 gcatttattt tttggccttg taattatccg tatattgttg tcctaaaatt ctcgccttac
      181 tcagatcctc tgggttcata gatttctcaa gatcactggc attgctcgcc gctttttcaa
      241 agccattatt cttggctgct gtaaaccaag cataagcttt tacaggatca gcagcaacac
      301 caataccgtc tctgtaatag atacctaatt gattttgagc cttaccatac ccttgctcag
      361 ctgctttttg catccagtat acggattgct caaggctttt accgacccca tcaccgtaaa
      421 aatacatatc agctaaatta aattgcgcat tggcattccc cagatctgcg cttttttgat
      481 accaaaaagc agccttttgc agatccgatt tacctaattt tccagaatca tataaataac
      541 ctaactccaa ttgcgatttg gcatgacctt gattcgctgc tttctcgaaa tactctgctg
      601 ctttctgata atctttagga acaccatccc cttgctcata catcaaacca agattatatt
      661 gtgcgctggc attacctaag tcagctgctt ttgcatacca gttgagagct tgagtcttat
      721 cctttttcac ccctgtaccg gtatcataat tataaccaat agccaaagcc gcattagagt
      781 tgccttgctc tgctgctttt ttataccata ccatggctgt atcggaattc tgaggtacac
      841 cagttcctgt atcatacata tagcccaagc catattgacc atccgcatta ttttgatccg
      901 ctgctttctg ataccattcg aacgcctttt tatagccctc tggtgtatta aggtaccaat
      961 agaaaccggc taacaatacc tgggcatcga cattgccttg attcgctgag gcaagtaata
     1021 attgctctgt tttttccgga ttataagtta caccttgggc attataatac atcttaccca
     1081 gcaaatattg agccttggca tctcctttat ccgcttcagg agacaacaaa ataaatgctt
     1141 tttcgtaata ccccttgtta taggcctcct caccttcctg agtcgtagcg atagctgaag
     1201 agttaatcac cgtcagtaga gaaagagata aaatatattt tttcatgtcc atgatatata
     1261 ccttattttt aagagctatc gctttaaagt atatgacata aattcagcaa tgtataaaat
     1321 tacttttcat tttgtttttt ttatatcttt catcaagata ttataactct tgcataacgt
     1381 gattattgtt ttagggaaag gtacaaacgt tttgaggagc ggactttata tgcagtaaac
     1441 gcgcacaaag tggcaataac atgaaaaaga cagattatct ttgcaatccg atattggagg
     1501 gctggttatt gaggtagcag gctattcatt gacaaatcat tcgaatggcc aggcattttt
     1561 tccttatgag ttctcactga acttgtagtt tgaaaaaaat ctggtttaaa aggacttaaa
     1621 gtctctatcc ccattcctaa taaagaacca atgagcccca tattggcaaa tcgcttgatt
     1681 gtagcgcctt gttgatttgt tccaatgagt attttattga cgtaactgaa acagtcatta
     1741 tcaccgggtt tcattttagt taatatgaaa gacgctgtcg gtggattcgt ttcctgaagt
     1801 tttttatgat aggccaacat ttctacaatc tttttaccac ttactgttct tgtttcgctt
     1861 tgagctggag cttctttggt atcggttggc tgaggggtaa attctattaa atactcacga
     1921 tttttaatat taatctttat atacgcatgg tgacctacac acccatgaag caaggattct
     1981 ccaataaaac tggccagaaa ggagttaatt tcataatcga tcttatgatt tagaaactcc
     2041 tcatactcgc tatcatcatt gaaaagctgc ttaatttcag tttctacttg gccaagataa
     2101 aatgaaaatg gtttaatttt atttggacca aacattgatg aggaatattc ctgcttgatg
     2161 ttgtccaagc tttcacctaa accatcaaga ccaaatttta tttgccgctg tatctcattc
     2221 ttaaatagct ttttcggtgc cctgaaacca aaaagcccac caagaactgt gccggtaaat
     2281 gttccaacaa gaaaacctat aactggtttg tactcacata aaccgttaac aaaacccagt
     2341 attcctccta acaatccacc gactacgctt aaagctacag ccgaaataat accgccaata
     2401 tgcagtaaaa caaccatcac ttgagcagtg aaatgatgag tcttggccac ttgttgcaat
     2461 tcacagaggt gaagcattaa ttctttgagt aattggcttg atgggcattg attatctaaa
     2521 aaattggaag tttttaattc ttttaattta tacaaggtat ccagcaattt tagcagtact
     2581 gctaaaaatt cgtgcctgga tgggacattt tgggatttta tcgattctaa caaaatatat
     2641 agataatact taaactctcc tgcaggcaag tcgataatac gctgtctcgc aaaatctatc
     2701 ttcaagaaat aatttttctc atccaccatg acaaaacaaa cctaagggga aacataaccg
     2761 aaatacacga tttaaaataa tactacaatc acaccatttg atcaaaaaaa aatcactatt
     2821 ggacaacgat tctgaaaacg ataatcacag ctactattac cagaatgata gctgccaaaa
     2881 ttgactaaat catactgaaa agggcaaaaa aatattaata gaattgcttt ttcagtacga
     2941 tgaagtcaat gcaaatggag tcacttataa acttcatggc aagcccatga gagaaaaaca
     3001 ttttgacttc aagaataccc tcattaaaga actaaaaata caacttgatt tgttagaaac
     3061 acccgaggac aataaaaatt ggtaggaatt aattatgcag tctattgtgg gcgctcaggt
     3121 ttaggcggag cacgctcacc ctcagcccca gcattcagaa ctccatggat tggtgaaaat
     3181 ttaactgcta tcaaaacatt gtgcgaaata aggacgaaag attttattaa tctcagatct
     3241 aacctcgagc aacagacaat tattgatgct ttacaacgag tttatcgaaa atgatgggat
     3301 taagataggt tactttaatc ctatttttat aattactcac ataatttaac taatggaaaa
     3361 tatatatcat gaaagaacct cgtgaactta atcgccaacc caatccagaa cacatggctc
     3421 aactcattaa agacacgccc aaagacgttt ggtttcatct cgcaaaatat cttcctgacc
     3481 atgaattact caacctcatg caagtcgaca aacgcttaaa tcgcttcttc aaagaaggta
     3541 cccccccaaa agaaataata aaaaaaatag aacacgcccc ccttaaggtc gtttcttcag
     3601 gcgtcaatac cttcattcta acccaatacc acggcaaacc actcttactc gcctgcggac
     3661 aaaacaaaga cggtcaatta ggctgtgggg atacgaaaat tagaacagca ctcacccctg
     3721 tcaatatccc tgaatcattt gcctccatcg agtcggtgca gatcagtctt ttccatgcag
     3781 tgctttgggg acgtgataaa gacaataagc cgatactagc gacttgcgga ttaaatgata
     3841 aaggtcagct gggttgtgga gactataagt ctagagcatt attcacccaa gtcaaattag
     3901 cagaagacat cacttccatc gaatcagtag aaatcaacat tttccattct gtgattttag
     3961 gccatgataa acataacaga ccagtacttg catcctgtgg aagcaatcac ttgggtaaat
     4021 taggttgtgg agacataaaa gataaaacaa cattcacccg aatgaaatta cctacagaaa
     4081 ttgtttccat agatacttta cagatgaatt ggcatacaat tgtttcagga cgcgataaga
     4141 ataacaaacc agtactggca gcttgtggat ggaatgatag aggccaacta gggtgtgggg
     4201 ataaaaagga aagagcatta ctcaccccag tcaagttacc tgaaaacatc gtttccatcc
     4261 aatcagcaca aattggcacc agtcgtacta cagttctagg tcgcgataaa gacaataaac
     4321 cgatacttac ctattgtgga tggaatgaaa gaaaccaaat gggttggagt aacactgaag
     4381 ataaaacagc actcacccca gcaaaattac ctgaaaacat cgtttccatt gagttaatgc
     4441 aaatcagtac tagccgcact gtaattttag gtcgcgataa aaacaacaaa ccaatattgg
     4501 ctgcttgtgg gctaaataat cacggtcaac ttgggtgcgg tgatattaaa accagaacaa
     4561 ctctttatcc aatcaaattc cccaaagaca ttgtttctat tgagtcggtg caaatcagtg
     4621 ataaccatac ggtgattttg ggaagagata agcacaagaa tccgataata gcagcctgtg
     4681 gacttaataa ttacggtcaa ctaggctgtg gggatacaaa aaacagaatg ctactcacac
     4741 ccatcaaact gcccaaagag atagtttgca tagactccgt gacagtaaaa tacagcagcc
     4801 tttttgtttc aggtcgtgat aaaaaccatc gtcccatact ggcggcttgc ggggataatc
     4861 gctacggtca acttggggtt ccaagcaaga aacttctaac cgaactgacc atcgttccca
     4921 cacccattct cacattgcct gcaacaccaa aaagagaggc ttcttccagg aaagaggcta
     4981 cagagaaagc tctagtcttt gctacccatg gcagcagaga gcaaaaaccc cgaaaaagca
     5041 ataggatact cactgcaatc attgcatcat tcggtcgttt ttttactgga gcacatcctc
     5101 atactccgga ctccgataaa gaaagccctt cacccaaacg aagcgcctaa gcaaaacatg
     5161 cattttagaa gcaagaggat ccaaaagtcc tcttatgctc gttcatcaat tgattgtttt
     5221 ttgaattcca tttgtcctat tgtttgatac aatcgtcata atttaaacaa caatgataaa
     5281 ttctttcagg gcctgtttac atttgtcact cagtcgatat gttgaagctt tattttcagg
     5341 tatgttatct ctcagcaata acctcctgag agataaattt cggtttggtt tgcccatgca
     5401 tgccagctaa taatttctcc tttggaagtt gcaacaaggt cacgggcaaa acttcttcgg
     5461 tttccacact atgagcataa gtcgggctaa tatggattaa ttgctcatgc agttttttca
     5521 gtggtaactc agaaatctct gcctcatcat cagtcactaa aacaatacga ttcccaacac
     5581 tattgcgcgc aaaataccaa taaggctcta ggttgatcag ggttgcctgg actgcacttt
     5641 gcagttcctt gcctgtaatt gagcaggttt ccaatttcag aagttgagtt ttataacaaa
     5701 actcaagctt tggtgaacta ttcaaatatc cggtacattt cacaatatct ttgagtctgt
     5761 agcgaacaaa tcccatagct gttgtcaaaa atacttcgta tttcttccct acttccaact
     5821 cccaacattg taaaagattc tctttttcaa ttgccgcccc ctctggaata aactccacaa
     5881 tatgggcacc tggatgtaaa attccaccca cgctgttagt ctctacagga actgttaacc
     5941 acccctcggt tgccgaataa gtaccgtcta ccagtttaat ctcagaaccc agcaattttt
     6001 gtaattgctg agcgggatac tcacaaagac cagaggtcca gcatccagcc atttccagtg
     6061 atggccacag ttgtttgaat gagagatggt caaccttggc taaagcctgc aaatgtcttc
     6121 gtcgtttccg ggttattttg agaggtggaa gatgtgatgg aagaagttta tctcccaata
     6181 aataaggaag atagtcttta aaaccgtcga tgcagcgttg ataaaaagca tcgattacca
     6241 tcggcgtcac cgcaaatacc gcacttaaat cagaagccaa accatagatg ggtccccact
     6301 ggttgtatgc ttcatggctg gcaaaaacct catcaggcat ggcataaaat tttttaataa
     6361 aggaaggcaa atgacgataa ttaaaattac taatccatcc cgtaggaata ccggcaggag
     6421 tcgttttatg agtatctacc gcgacaagat acaacatttt ttccttaaaa aagccgggga
     6481 atcgctgagt tagactgtaa atatacgggg ccattgtacg ctgaaattgt ttctgaaatg
     6541 aatctgttat agggaaaaac tttctaactc ctgatgttcc tgaagtttct gaccaaaaaa
     6601 tcaattcctc accattaaaa ggttggatag tagaatgctg ggctgccaac aatccttcct
     6661 gataatcttc atattctgta attccaaaat cattaagaac tatagaagat tgatctttta
     6721 ataattcctg ccaataagat gattttttca gcaacgggac aatttctgaa ttccataatc
     6781 gttctctggc ctctttagga tgtttggtat cattcagaaa aaattggtat tttttctttg
     6841 tcatggcagc aattaaaaac cttttccaat tcatccttca ttaccccgca atatgtattg
     6901 gcataataga aaacagctgt ttttcctgat aaaaagaata caaacgctca ctcatagctt
     6961 cttgtaacaa gctatcaatc atcgaacatg aattcaatcg cttaagaatt aactgcatag
     7021 ctaaatccag ccaatctgta ttttgcaaat catcagaaag tatctcctga ttatagtgcc
     7081 agaactgcaa acaacagctg gctgcaaaaa tccagcaata tttttctgcc aaacgaaaaa
     7141 cagttaagct gcgtgggtca aataatttct gatcatgtaa atggataact tcctgatcca
     7201 atttttcaat ttcatgacgt attgtagtaa tcagtggatt gattgcaggt gagtttaaac
     7261 gcatgatacc agccaataca tcatcttcct catgagtaaa caaaccgagt ctatcctcct
     7321 taaaaggagg acaagacaaa cgaacattaa aaattttctc aattttttcg gaatgctctg
     7381 cctgatgttt accccgcatg cctgcctgag gcaacaaatt accagcaata agtgataaat
     7441 taacttgagt acttccatca aacaagccaa ccacttgaat gtcgcgtctt attttctgaa
     7501 aaatcgccca ttgcgtggtt ctcagataag ctctggcacc tagaataatg gcacactgtt
     7561 ctgctatatc ttctccaata tgcggaataa gaaatttaat aattgctgac caaagactca
     7621 tttttttcgg tattaccgta caggcgcgaa ccataacctg agcagtgcaa tccgcgatta
     7681 ataaaagagt aaacaattcg ccaagccgct gttttactgc gggtatttca aacactgatt
     7741 tgccatacag ttgtctttcc aagctatatg ataatgccag cctcaaagca gtatcagcgc
     7801 ccccaatagc caaacaggcg cataaggttc gcgaaatctg gaatacctta taggttattt
     7861 ccaaaccacg ttgttccttc cctattaatg cgtctttaga tactttcact ttatcaaaag
     7921 aaaaaccact gatatctaac cctctgactc catgagtcgg cagcttgggc gtaggagaaa
     7981 tacccttttc ggctaatttt ttatcgatat aaaacaaaga aaaacctaat aacccgccct
     8041 tttcattagt acgacacaac accacagcgt actcgctcaa ggttgcaaaa ttaacacacc
     8101 attttcgccc cgaaatttcc cagccatctt caattggctt ggcattcatc ttattggaag
     8161 tcaaatcagt tccatgctct tcttcggtca aagccaaagc acctatcttt ccctggcgaa
     8221 ggctctcact caaatgtttc ttttgctttg agtttccagc tatccatatt ggcaatgcag
     8281 caaagaaagc caatccaaac ataatagcca tcgtgatgtc acgtctggcc aacaacctgg
     8341 ttaaggtata tagctcatcc agggaactca gcttaccccc tagataatga ggtatggtat
     8401 agtccaaata cccccattgt ttaataaatt ctattgctga cgaagctaat acttcctgct
     8461 catcagcgag caaaatttgt tgaaaattaa caggggaatt gttttccaga ggattaccca
     8521 ggcatgactc aaaaaattca gacagttgta tcaaattttc ataagaggtc attattttcc
     8581 ttatacaata gagtgttttg ccgggggcat ggtttccatg cttctatata aaaactccaa
     8641 atgggaggca agacgactgc gaatttgcgc tgcctggata atttcatcaa tcaagcccaa
     8701 tgaataagcc tccatgaggg aatcatcaat ttgagacttt tctgtcaatt gggattgtga
     8761 tgattcctgt actggttgac catccatggc ttgcacctta ttagttgttt ccttccccat
     8821 gacaccaaga tttgctgttt ccagagccaa aaccaaatcg ccattctgtg atttggtttg
     8881 catcatgaga aaggctgccg ctccataaca tttacgcacg atgacactta gccttggcac
     8941 tctggtttgc atagctacgc aaaaccttgc accatgctgc aataatccct tttgctcctc
     9001 tcttttaccg ggcataaaac cgggaacatc gataaaagtc aaaattggaa tagaataagc
     9061 atcacatatt cttataaatt tggctgcttt ttgagctgca tcggaatcga tggctccgct
     9121 gtaacgaatg ctttgattag cgacgacccc aacagcaaag ccatgtatat gcacaaaagc
     9181 acaaatcatc gcctgtccat aagtcctttt gtactggata acgtttgaat tatccacgac
     9241 agcctgaatt aaattgagca tattaaatgg cattctcgga tttgcaggaa tatcaggaat
     9301 ggggtgatta ggctccatga taggatgaac tggcggacgt tcattggtat gtagaggaaa
     9361 aaagccaagc attgctttca catggtttat ttgtgcggta acactatcgt ccacgaaatc
     9421 agcaataccc gttgtcgttg catgcagtgt tgcgctcccc agctcgccca tagtcacttt
     9481 ttcactaatg gcttgttgta ctaccgtagg gctagtcacc ataagatagc ttctatgctt
     9541 gttaaaaaat aataagtcca ttaacacagc agaataggcc ggagctccaa gagtgggtgc
     9601 cagtatcact gcaaattgtg ggataattcc tgacagtcta atatttcgag caataatttg
     9661 agtgtattcg tcgccactca gcaaattttc ttcgagagaa accccgggag atgctaaaaa
     9721 ggcaacaata ggaatgcgta atttttccgc cttatccatt aaatgaataa tctttctagc
     9781 gccttgtcgg gtaacatacc ccctgttgac tgaagaatca tgagcataaa tcgctactgg
     9841 cctttgattc actttggcta atccggtaat cacctcagcg ccaggaaggg aatgaagatt
     9901 attatctgga atatattcag aacctattgg taaaaagctt tgttcatcaa ttaaattatc
     9961 tatccattta attaacgtct tatctgtatg ttgggaagac atacctcttt cctcttgcaa
    10021 tatttaaatc ttggaatctg agactttgta acttggaaat ttgatcaata tccataataa
    10081 cttaattccc aaaataggct actcttaatt gtataagttt agagctattt atatgaaaaa
    10141 gataaaaatt cttgatct
//
LOCUS       DACTJE010000117        10137 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.117, whole genome shotgun sequence.
ACCESSION   DACTJE010000117 DACTJE010000000
VERSION     DACTJE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.117"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(155..1354)
                     /locus_tag="I9760_002169"
     CDS             complement(155..1354)
                     /locus_tag="I9760_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HAT5043932.1"
                     /translation="MIKSFRVTVFLFLVSGLILARDMHAFPSGLESNQDKLVYFQLEA
                     ETFYSPKPVTIEGQLYLTYEVFLANVGDYLFSLENVEVLNGRKPYKVLFSYNQEELRK
                     MIYSFSGHSNHLTSDLTLRPGERGLLFFMVKFDSLDEIPEKIMHRFHLYSSKTKISEE
                     GVRYSFLAAPAYVKITKPLVIANPLDGKHWMAINGPSNHSIHRRARVAAHGIVYYPER
                     YAIDFMQFGADGHVYKENPNKNENYYSYGANVYSVADGEVVAVYDGVPDNTPPGRDYP
                     VTLINMAGNHVIIKIGKHQYALYAHLIPHSIKVTKGELVRKNQLIAKLGNSGNSDAPH
                     LHFHVIDKPSPLIGQGTPYAFEHFSTEEYRFAGDEWESPIVYEGRLTEHHHELVHENA
                     LMNFAEK"
     gene            complement(1409..1981)
                     /locus_tag="I9760_002170"
     CDS             complement(1409..1981)
                     /locus_tag="I9760_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5043933.1"
                     /translation="MALIRDSKSQVMSVFKFQPLGIKDLNQLYQWFQEPIINHWYARD
                     KSWSLDEITEKYEPRIAGMEHVPSFIVYKDDLPFGFVQFYILTDSLPDGIDGYESFLF
                     EKYNPRDLAGIDLFIAESEGRGRGLGVELINRFIDKFLTRFKAVVVDPDVNNQQAIRC
                     YEKAGFQQTGFSQDPNHLIMIKSLSLITGN"
     gene            complement(2171..3517)
                     /locus_tag="I9760_002171"
     CDS             complement(2171..3517)
                     /locus_tag="I9760_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAT5043934.1"
                     /translation="MKKWIISVLLLVVIVLCFYPIIGMMALSTVKKNVDNIPRNPFLT
                     WQLKDNHRGWFCSTAVLNLLVDLPAQENKDAQGNVKVQPALHLSLDFPIVIHHGPFIM
                     TNNGLHFGLAWVTTKPETHYGALINYLNQTVVSYSLPSINMQGTNNGDQGNFVFVWQG
                     LSAKLKVNPKVDKASGHVTMYGMSTKVQETDVRIGKVKINFALNRYMDDLWLGDSSIL
                     LSSVSTITNSQPVFDLQDLSMDAWAGINNNLLDFNLQLSLNKFLMNNNSYGPGQLKIE
                     LKNLDPKAMAKLNQLNYDYPSNQIAILAEIFNLLSKGATLNLNQELDTPQGHLSSSLN
                     LALTKSSFSNPGDLLQNLKGNGQFKAPIKLVKQLLFESIKSKTATPAADMSQSQVTEP
                     LVDVDAEAHKQTEEILQKWVNKGILKVEGDYYQASFTLENTQFTVNGQPFDPALLN"
     gene            3810..4874
                     /gene="legL7"
                     /locus_tag="I9760_002172"
     CDS             3810..4874
                     /gene="legL7"
                     /locus_tag="I9760_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL7"
                     /protein_id="HAT5043935.1"
                     /translation="MNYKLTLHPGSNPVEEFTSIPHGVTSLDLSLNNLYSISTVELIQ
                     AFANTPASVTSLNLSGNSLGFKNSDELVQILAAIPANVTSLNLSGNFLSYKSSDELVK
                     TLAAIPFTITVLDLGWNDFSSKSSSEFKQAFSNLPASITSLNLRGNDLGIKSSDELIQ
                     ILAAIPANVNSLNLRGNNLASKNCAELAKFLASIPASVTSLDLSANLLGLKSYAELAY
                     IFSSIPNHVVSLNLCLNCLHGPSLENLKLLKDSLKHLQTVYLDYDIVKNMSKEQCKAL
                     GAAFPNIQKIILVDKNGKEIHPSHSIPISNLIRELSGKADVPSLLNQCLIFAQKHQTN
                     IEDLNIPDELRESIQTCKPL"
     gene            5095..6609
                     /locus_tag="I9760_002173"
     CDS             5095..6609
                     /locus_tag="I9760_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAT5043936.1"
                     /translation="MSYMKIEELLLKDTMKKGQIPGVSIAYINNQGIISTQEIGFTDG
                     CGLVKMSKDPTQCPFQELVLGSKNELGIGTKNTVIAFNKELYYVDQAAKMVQKIALTE
                     ANKIAYEMLHAKCTETYQLADGNEHEFIAALTGRMPPTEVKPDTVFGAASLSKPLFAY
                     LVQKLVQANVSNTSQTGYDQFILPDTLKSFDLDTPLFHIIPLEEFNIDGMKFDLSDES
                     AAAHSKALTARMVLSHTTGLAHGEMKFQFLPNSQEKEHGYSNVGIIYLQQVIEKLTGA
                     DLETLAQKHVFQPCGMVHSTYGPKPCAANSLWTTAGDYANFVKHLIHDSTIENPFVPH
                     TYMTKDKGLAGATGIAKGNIPDTILQHVAWGLGWGLQTNDEGRVITAYHSGDMNDYRA
                     WVVIDLQDKDKKNAVVFFANSHNGHILAEQIIPKTMQMEQAANYFFSKWGFARNFAEL
                     GGKTTRLGIKSSSGSSDSSPDTSIQDEPVEIKKETEKSDTSSIETYTSGWTFNA"
     gene            complement(6586..7506)
                     /locus_tag="I9760_002174"
     CDS             complement(6586..7506)
                     /locus_tag="I9760_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT5043937.1"
                     /translation="MAIPFKRINLNLILHLDALLSERSVSAAAEKVFVSQPAMSSSLK
                     QLRELFKDPLLIPGKGEYFLSPKAKELQPQLEQVMFFIRNILQPNERFDPFQTKRSFK
                     IALPDYVELILLPSLMAALEKLPHIVIETTIQSMADDPHLFIDENVDLAIGAINENSL
                     SAQLKVEKLYEEEIICFASKNNPLINRPLSLEDYISNKHIAFSFNQERYSHSAEYLYK
                     RKLVRNDMLFVKNILPAIFAVANSDAVISTAPSFLINMFARQFNLGMQPLPFNLEPTP
                     IYLVTHSKSQYDEGVSWLAKLVSSSIKSPS"
     gene            complement(7691..8230)
                     /locus_tag="I9760_002175"
     CDS             complement(7691..8230)
                     /locus_tag="I9760_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043938.1"
                     /translation="MFGELFASSIVLGVRYYSNQAYQAVAGLLGQIERFGIGYTREFI
                     RDPKSMIQSLTSKARSSEAGYSVVSLGYLGGNYAVITPKSQSQLTELLNQFDQEKSGR
                     RGHASFKFFSGNDFFIISDIPFSVGLRNCPAFAVTESLFKVTIAKFVSDYQLQLVKKD
                     EDDSTLHITSRDKSFGLMQ"
     gene            8527..9441
                     /locus_tag="I9760_002176"
     CDS             8527..9441
                     /locus_tag="I9760_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043939.1"
                     /translation="MATTLIQTPSYTKNLTLNLDDYPGGVAIWGALPALFDTSNQGFD
                     RGVHVHARLADSSKKVIDATYDHVTVISGYRIFTITEEAAVHFSMSAIFDIKITSLTC
                     QHCSQLITSVGYAAVRPSRQHQCNHCGEITTTTSDCISNPIMLLKELIGDEQVKRPAV
                     IPNRTIAIDPDKYSGGIQIWGSNPSIIWTAKRLEESAIHIHAYNENGKRIIDNTYGSV
                     SLDGHKLDIEMIRVLQIQLALPNLALLLTTVYCPHCGAEQFDRGIWAVSAHNHRVCLL
                     CKQTFISQDVISNPAFDVLTHVSGVISQ"
     gene            9438..9839
                     /locus_tag="I9760_002177"
     CDS             9438..9839
                     /locus_tag="I9760_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAT5043940.1"
                     /translation="MNTLKTIFKIGALIFNEKNQLLAVHKKGKPPMELIVPGGVMEEN
                     ESDEETLRREIKEELDSDIISFQFYKQFEDKAIYEEKWLVMRTYIVTLGNAPKPANEI
                     DQLVWLGHDYHQSGYRFGSILGKQIIPDFFK"
     gene            complement(9923..>10137)
                     /locus_tag="I9760_002178"
     CDS             complement(9923..>10137)
                     /locus_tag="I9760_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043941.1"
                     /translation="TCFLIEETKLASQINRKMMADIARHEGLSGKRLVFFKESSPKVT
                     TEVRHCSDAERDIHDQQFGLVPCLTK"
BASE COUNT         3175 a         2060 c         1889 g         3013 t
ORIGIN      
        1 acaacgacct gtcttatcca caactcacgt tacctcatat tatagactat gggacattcc
       61 atgttatgag catcaatttg ccagggaccc atttaatact tcatgtataa aagcgtcttc
      121 ctaaaatact ctatttttat cgtctaatat tcgattattt ttctgcaaaa ttcatcaaag
      181 cattttcatg aaccagctca tgatgatgtt ctgttaatct cccctcatag acaatagggg
      241 attcccactc atcgccggca aatcgatatt cttctgtaga aaaatgttca aatgcatagg
      301 gtgtcccttg gccaattaaa ggagatggtt tatcaataac atgaaaatgc aaatgcggtg
      361 cgtcagaatt gcccgaatta cccaattttg caattaattg attcttacga accaactccc
      421 ccttagtgac cttaatacta tggggaatta agtgggcata gagagcatat tggtgttttc
      481 ctattttaat aatcacgtga ttgccagcca tattaatcag cgtaacagga taatcacgtc
      541 caggcggagt attatcagga acaccatcgt aaactgcgac tacctcacca tccgcaaccg
      601 agtaaacatt ggccccataa ctgtaatagt tttcattttt gttgggattt tctttataga
      661 catgaccatc agctccaaat tgcatgaaat caatggcgta tcgttcggga taatacacaa
      721 tgccatgggc tgcaaccctt gcgcggcggt gtatggaatg attggatggg ccattaattg
      781 ccatccaatg ctttccatcc aaaggattgg caatcaccag gggcttagta atttttacat
      841 aagcgggagc agccagaaaa gaatacctga caccctcttc ggatatttta gttttcgatg
      901 aatatagatg aaaccgatgc attatttttt cagggatttc atcaagacta tcgaatttca
      961 ccataaaaaa taacaaacca cgctcacccg gtcttaatgt caagtctgat gttaaatgat
     1021 tagaatgacc actaaaagaa tatatcatct ttcgcagctc ttcctggtta taggaaaaaa
     1081 gaactttata aggttttcgg ccatttaata cttcaacgtt ttctaaagag aagaggtaat
     1141 cacccacatt ggcaagaaaa acctcatacg tgagataaag ttgtccttca atagtcacgg
     1201 gttttggaga ataaaacgtt tcggcttcta actgaaaata aaccaattta tcctgattgg
     1261 attccaatcc agagggaaaa gcatgcatgt ctcgtgcgag aatcaaacca cttacaagaa
     1321 ataaaaaaac tgtgacacga aatgatttta tcatttaaaa cccttggtaa attgccaaaa
     1381 caacgttcta ttttcaatag gctcatcatt aattccctgt aatcaatgac aaggatttta
     1441 tcatgatgag atgattggga tcctggctga aacctgtttg ctgaaagccc gctttttcgt
     1501 agcaacgaat ggcttgttga ttgttaacat cggggtcaac aacaacagct ttgaaacggg
     1561 taagaaactt atcaataaag cgattaatga gttccacccc taatcctcta cctcgtccct
     1621 cgctttcagc aataaacaaa tcgattccag ctaaatccct gggattatat ttttcaaaga
     1681 gaaaactctc ataaccatca attccatcgg gtaaagagtc tgtgagtatg taaaactgga
     1741 cgaacccgaa aggcaagtca tctttatata caatgaaact tggaacatgc tccatacctg
     1801 caattcttgg ttcatatttt tctgtgattt catccagtga ccaacttttg tctctcgcat
     1861 accaatgatt aataattggc tcctgaaacc attgatacag ttgatttaaa tccttgatgc
     1921 ccagaggttg aaatttaaaa acactcatga cttggctctt tgaatccctt atcaatgcca
     1981 tgacgactca catctgaggc atgccaaatt gatttccata tgtcaggttt gcggataatc
     2041 atgattcact atactgtagg caaaaactga gtgggtattc taccacagtt ttcatcgcct
     2101 ggatttgctt gtgattgaca tagaagatca tctcatcagt gcaggttgag caaattgccc
     2161 aaccgctaat ctaattcaat aacgctggat caaatggctg cccattcact gtaaattggg
     2221 tgttttccag agtaaatgag gcctgatagt aatcgccttc aacctttaag atgcctttat
     2281 tcacccattt ttgcaagatt tcttccgttt gtttatgggc ttcagcatcg acatcaacca
     2341 gcggctctgt aacctgagat tgagacatat ccgctgctgg tgtagctgtt tttgatttga
     2401 ttgattcaaa taacagctgc ttgaccagtt tgattggcgc tttaaactga ccattgcctt
     2461 tcagattttg caacagatcc ccaggattgc tgaatgaact cttggttaga gccaggttca
     2521 acgaactgga caaatgcccc tgtggcgtat ccagctcttg attcagattg agtgtcgcac
     2581 ctttggaaag cagattaaaa atttcagcca ggatcgcgat ttgattggaa ggatagtcat
     2641 agttcaattg attgagcttg gccatcgctt taggatccag atttttaagc tcgattttta
     2701 attggccagg accatagctg ttattattca ttagaaattt attcagggat aattgcaaat
     2761 taaaatccag taaattgttg ttgattccag cccacgcatc catactcaga tcttgcaaat
     2821 caaacactgg ctgtgaatta gtgatggtag aaacagaaga cagcaagata gatgaatcac
     2881 caagccataa atcgtccatg tagcgattca atgcaaaatt gattttgact ttaccaatcc
     2941 tgacatcggt ttcttgcact ttcgtactca taccgtacat agtcacatga ccagaggcct
     3001 tatcaacctt gggattgact ttgagctttg cacttaagcc ttgccagaca aaaacaaagt
     3061 tgccttgatc accattgttc gtgccttgca tattaattga gggcaaacta tagctgacaa
     3121 cggtttgatt taaatagttg atcaaagccc cataatgggt ttccggcttg gttgtgaccc
     3181 aggcgagacc aaaatgcaag ccattattgg tcatgatgaa aggaccatgg tgtatgacga
     3241 ttgggaaatc gagactcaaa tgaagcgctg gctgcacttt aacattaccc tgtgcgtctt
     3301 tattttcctg agctggcaaa tcaacaagta aattcaaaac ggcagtggag cagaaccatc
     3361 cacggtgatt atcttttaat tgccaagtca ggaagggatt cctgggaata ttatcgacgt
     3421 tttttttcac cgtgcttaag gccatcatgc caataatggg gtagaagcat aagacaatga
     3481 ctactaacag caacacactg ataatccatt ttttcatgac tgtccaacat taatccttag
     3541 taattaatat ttagaccaga aaaagctgat tgtaaaatag cccatctatc tgaaagaacc
     3601 tttaatactt gagcgagccg gctgctcaag tcttccctag caaactttca atttctaact
     3661 ggattttcaa tagaacaaat ccactttaat ccagcattta taagaaattt gtatacacaa
     3721 tataagaaca attgatttta atttagaccc catggtggat attatgatca ttttgatcaa
     3781 acaaagacta tcatttcgag attttcataa tgaattacaa gctaacatta cacccaggca
     3841 gcaacccagt agaggagttt acctctatcc cacatggggt cacttcactt gatttaagtt
     3901 tgaataactt atacagtata agcactgttg aattaataca agcctttgct aatacacccg
     3961 ccagcgtcac ttcactgaat ttgagtggaa attccctagg ctttaaaaac agtgatgaat
     4021 tagttcaaat tctcgcagcc atccctgcca acgtcacttc attgaatctg agtggaaatt
     4081 tcttaagtta taaaagcagt gatgaactag taaaaaccct ggcagccatc cctttcacca
     4141 tcaccgtact ggatttaggc tggaatgatt tcagcagtaa aagcagctca gaatttaagc
     4201 aagccttttc caatttacct gccagcatca cttcactgaa tttgagagga aatgacttag
     4261 gcattaaaag cagcgatgaa ttgatacaaa tcttggcagc tatccctgcc aacgtcaatt
     4321 ccttgaattt gagaggtaat aacttagcca gtaaaaactg tgctgaattg gcaaaattct
     4381 tggcttccat tcctgctagt gtgacttcac tggatttaag cgcgaatctc ttaggcctga
     4441 aaagctatgc tgaattagca tatatctttt cttctatccc aaatcacgtg gtttcactta
     4501 atttgtgcct taattgtttg catggaccat cccttgaaaa tttgaaactc cttaaggatt
     4561 cactcaagca tctacagact gtttatttag actatgatat cgtcaagaac atgtccaaag
     4621 aacaatgcaa agcactaggt gccgcattcc ccaatataca aaaaatcatc ctggtcgata
     4681 agaatggcaa agaaattcat ccgtcacatt ctatcccgat ttctaatctc attagagaac
     4741 tctctggaaa agcagatgta ccatccttgt taaatcaatg ccttattttt gcccaaaaac
     4801 atcaaacaaa cattgaggac ttgaatattc ctgatgagtt aagggaaagc attcaaacct
     4861 gcaaacctct ctgatttatg gtcttgaagc ttccttctat tgcttttgtg aataagttaa
     4921 aaaagcgatg aagtcaggat taattaatcg tctgcattat gttgtagcaa tgcagcaaaa
     4981 accggggtat taacgggttt ttacgatatt tttgcgaaaa aaatttttga gtaaatcgaa
     5041 cacgttaaca cccaaagtat aatttatgac caaattattt ttttgagtga acttatgtct
     5101 tacatgaaaa tagaagaact tcttttaaaa gataccatga aaaaaggcca gatcccgggt
     5161 gtatccattg cttatatcaa taatcaagga atcatttcca cccaggagat aggttttaca
     5221 gatggatgtg gccttgtcaa aatgtcaaaa gatcccaccc agtgcccatt tcaggagttg
     5281 gttcttggat ctaaaaatga attaggtatc ggaaccaaaa acacggtcat tgcatttaac
     5341 aaggaacttt attatgttga ccaggcagca aaaatggttc agaaaattgc gttaacagag
     5401 gcaaataaaa tagcctatga aatgttgcat gcaaaatgca ccgaaaccta tcaattggct
     5461 gatggcaatg aacatgaatt cattgcagct ctaacaggtc gcatgccacc aaccgaagtg
     5521 aagccagaca ccgtttttgg cgcagcatct ctaagcaaac cactctttgc ttatctggta
     5581 caaaaattgg tacaagccaa tgtaagtaat acatctcaaa caggatatga tcaatttatt
     5641 ctgcctgaca ctttaaagag ctttgatttg gatactcccc ttttccatat tatccccctg
     5701 gaagaattca atattgacgg catgaaattt gacctgtctg atgaatctgc cgctgctcat
     5761 tctaaagcac taaccgccag gatggtgtta tcgcatacga ctggactagc tcatggggaa
     5821 atgaaatttc aatttctgcc caactctcaa gaaaaagaac atgggtattc gaacgttgga
     5881 attatttatt tacaacaagt gattgaaaaa ctgactggtg cagatcttga aacactggcc
     5941 caaaaacatg tgtttcaacc ctgcggtatg gttcatagca cttatgggcc caaaccctgc
     6001 gcagccaatt ctctatggac tacagcagga gattatgcca atttcgtcaa gcatttaatt
     6061 catgacagca cgattgaaaa cccctttgtt ccccacactt acatgacaaa agataagggg
     6121 ttggcaggag caaccggaat agccaaaggc aatatacccg atactattct gcaacatgtt
     6181 gcatggggtt taggctgggg attacaaacc aatgatgaag gaagagttat cactgcctat
     6241 cattcaggcg atatgaatga ttacagagcg tgggtagtga tcgatttaca ggataaggat
     6301 aagaaaaatg ctgtggtatt tttcgctaat tcgcataatg ggcatattct cgctgaacaa
     6361 attattccga agaccatgca aatggaacag gctgccaatt attttttttc aaaatggggt
     6421 tttgccagaa acttcgctga attaggcgga aaaactactc gactgggtat taaatcatcc
     6481 tcaggtagct ctgatagctc tcctgatact tccattcagg atgagccggt tgagataaag
     6541 aaggagacag aaaaatcaga tacttcctca atcgagactt atacttcagg atggactttt
     6601 aatgcttgaa ctgaccagct tggctaacca ggagactccc tcatcatact gacttttgct
     6661 atgggtcacc agataaatag gagttggttc cagattgaaa ggtaaaggtt gcattcccag
     6721 attaaactgt ctggcaaaca tatttatcaa aaaacttggg gctgtggaaa tcaccgcatc
     6781 ggaattggct actgcgaaaa ttgcgggcaa gatatttttt acaaaaagca tgtcattacg
     6841 taccagcttt cttttatata aatattcagc ggagtggcta taacgctcct gattaaaact
     6901 aaatgcgata tgtttattgc ttatgtaatc ttccaaagac aatggcctgt ttatgagtgg
     6961 attgtttttg cttgcaaaac aaataatttc ctcctcatac agtttttcaa ctttgagttg
     7021 cgcggataga gaattttcat taatagctcc aatcgccaga tctacattct catcgataaa
     7081 cagatgtgga tcatcagcca ttgattgaat cgttgtctca ataacaatat gaggcagttt
     7141 ttcaagcgcc gccatcaggg atggcaacaa aattaattca acgtaatcag gtaaagcgat
     7201 tttaaatgat cttttggtct gaaagggatc aaatcgctca ttcggttgca gaatatttct
     7261 tatgaaaaac atcacttgct ctaattgtgg ctgcaactct ttagcttttg gagagagaaa
     7321 atattcccct ttaccaggaa ttaatagcgg gtctttaaat aactctctca gttgttttaa
     7381 agaggaactc atggctggtt ggctgacaaa cactttctct gcagcagcac tgacgcttct
     7441 ctcgcttagt aaagcatcca gatgtagtat gagatttaaa ttaatacgtt taaagggaat
     7501 cgccattcga tcctctcggc aattgttctc ttcttatatt agtataagaa atttaaaagc
     7561 atccaatatg gataattcat tatcaatgac agagtgtgtt ttgagagggg ttacaatgcc
     7621 acttaccgaa ccaagtaacg ctgtcaaata gacctcatta cctggtatca atctattgtt
     7681 ctttccagtg ctattgcatt agtccaaaag atttatctct ggaagtaatg tgtaatgttg
     7741 aatcatcctc atctttttta accaattgaa gttggtagtc tgaaacaaac ttggcaatcg
     7801 ttactttaaa taaggattcg gtcactgcaa atgcagggca gttgcgcagt cctactgaaa
     7861 agggaatatc tgaaataata aaaaaatcat taccagaaaa aaatttaaac gaagcatgcc
     7921 ctcttcgacc agatttctcc tggtcaaatt gattcaataa ttcagtcagt tgagattgag
     7981 actttggtgt aatgacggcg tagtttcccc ccaaatatcc caaactgaca acactatatc
     8041 ctgcttcact gcttcgtgct ttagaagtta atgactggat cattgattta ggatcacgaa
     8101 taaattctct ggtatagcca ataccaaatc gttctatttg ccccaaaaga cctgccaccg
     8161 cctgataagc ctgattggaa taataacgaa cacccaacac gattgaactt gcaaataatt
     8221 caccaaacat aaatcatccc aaactatagg ccaaaaaatt ggactatagt aatacattta
     8281 gaaaatattg aacacatcca ggcgtttttt tatttcatgt tggctgatta tatgatgttt
     8341 attattcacc cctttcaaat accaggaaat gattcggaca ccgatacttg tcaatagtta
     8401 gcaggagcga tacaatgact ttgcctaaaa aattatggtc ttcagcaagt ctcgattcct
     8461 ccggtgctgg gtataaagac ataattttgg tcgagggata ctttcgtttt taatacgagg
     8521 tatcctatgg caacgacatt aattcaaaca ccctcttata ctaaaaatct aactctcaac
     8581 cttgatgatt acccgggagg agttgcaatt tggggtgcat tacccgctct ttttgatact
     8641 tcaaaccagg gatttgatcg aggcgtccat gttcacgcgc gtcttgccga ttcatctaaa
     8701 aaagtgatag acgcaaccta tgaccatgtc acagttattt ccggatatcg tatttttaca
     8761 attacagagg aagctgctgt tcatttcagc atgtccgcca tttttgatat caaaattaca
     8821 tcattaacat gtcagcactg ctctcagttg attacttccg ttggctacgc tgcagtcagg
     8881 ccatcacgcc agcatcaatg taatcattgt ggtgaaatca cgactacaac ttcagactgc
     8941 atcagcaatc caattatgtt acttaaagaa ttgataggag atgagcaagt taaaaggcct
     9001 gcagttatcc ccaatcgtac cattgcaatt gatcctgata aatacagtgg tggcattcaa
     9061 atttggggct ctaatccatc cattatttgg actgctaaaa gactggaaga aagcgctatc
     9121 catattcatg cttacaatga aaatggcaaa aggattatag ataatactta tggaagtgtt
     9181 tctttagatg gtcacaaact ggatattgaa atgattagag ttctgcaaat ccaattggca
     9241 ttgccaaatt tggcattact cctgacaacc gtgtattgtc ctcattgtgg tgcggaacag
     9301 tttgatcggg ggatttgggc tgtttctgcg cataatcaca gggtttgctt actatgtaaa
     9361 caaactttta tatctcaaga cgtgataagt aaccctgctt ttgacgtact aactcatgtt
     9421 tctggagtga tctctcaatg aacacattaa agactatatt taaaataggc gcgcttattt
     9481 ttaatgaaaa aaatcagtta cttgctgtac ataaaaaagg aaaaccaccc atggaactca
     9541 ttgttccagg tggcgttatg gaggaaaatg aaagcgatga agagaccctg cgtcgagaaa
     9601 tcaaagaaga acttgactcc gatatcatat catttcagtt ttataagcaa tttgaagaca
     9661 aggcaatcta tgaggaaaaa tggctggtaa tgcgtactta cattgtcact ctcggcaatg
     9721 cccctaaacc tgctaatgag attgatcaac tggtatggct tgggcatgat tatcatcaaa
     9781 gtggctatcg ttttggatcc atattaggca aacaaattat tcctgatttc tttaaataaa
     9841 ccattcccag acattcacag caacttttgg ctcagaccag gaagtgaagt ccgaaccttt
     9901 atgagatgag aataaaatga tgctatttgg ttaaacaagg cactaatccg aattgttgat
     9961 catgaatatc ccgctcagca tctgaacaat gccgaacttc agtagttacc tttggagatg
    10021 actctttaaa aaaaaccaac ctttttccag aaagaccttc atgacgggct atatccgcca
    10081 tcattttacg attgatctga ctggctaatt tcgtttcctc aattaaaaaa catgtag
//
LOCUS       DACTJE010000118        10007 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.118, whole genome shotgun sequence.
ACCESSION   DACTJE010000118 DACTJE010000000
VERSION     DACTJE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10007
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.118"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..904)
                     /locus_tag="I9760_002179"
     CDS             complement(<1..904)
                     /locus_tag="I9760_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAT5043942.1"
                     /translation="MFKPQGLYDYICQQWQEEILPSLCDYIKIPNKSPHFDAKWEEHG
                     YMEQAVNHIANWCKSHAPKGMTLEIVRLKNRTPLLFMEIPGQIDDTVLLYGHLDKQPE
                     MSGWSDDLHPWKPVLKNGLLYGRGGADDGYSAYASLTAIRALEQQGLPYPRCILIIEA
                     CEESGSYDLPFYIELLKERIGKPSLVICLDSGAGNYEQLWMTTSLRGNLVGKLTVELI
                     NEGVHSGSASGIVADSFRVARQLISRIEDENTGEIKLPQLYCDIPDERIKQAKQCAEI
                     LGEQVYSEFPWIDSAKPVIQDKQQL"
     gene            complement(879..1970)
                     /locus_tag="I9760_002180"
     CDS             complement(879..1970)
                     /locus_tag="I9760_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAT5043943.1"
                     /translation="MSPSIVFTGGGTAGHVTPNIALIKEFRKEGWNVEYIGSVSGIEK
                     EMIEPLDIPFHGVSSGKLRRYFSLKNLLDPFKIVLGIIQSSLLFYKIKPDVVFSKGGF
                     VAFPVVVGAWLNRIPVVAHESDMSPGLANRLSFPFVNKICLTFDAGKKYFKRQDKIEV
                     TGTPIRQQLLTGNRMKGLELCGFNSSKPCLLVVGGSLGAGSINSCIRSALKQLTSEFQ
                     VIHLCGKGKLDSSLVGAEGYCQFEYANEELADLFAASSVVISRAGANSLYEILALGKP
                     HILIPISSQVSRGDQIQNARYFQGLGISVVIQDELLKADVLLQAVQDVMRKKDEIDNK
                     IKALKIESATDKIVAIIKEQAHVQTPRIV"
     gene            complement(1967..2809)
                     /gene="nadC"
                     /locus_tag="I9760_002181"
     CDS             complement(1967..2809)
                     /gene="nadC"
                     /locus_tag="I9760_002181"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAT5043944.1"
                     /translation="MNNVLSQVTFDVKRALQEDVGNGDVTAALLPQQLIVEAEIISRE
                     PMLVCGQPWVNEVFKQVDNTVEMEWRVSEGDFLSTPATLCIIHGTASSILTAERTALN
                     FLQTLSATATQTYHYVQKLKGTKAKLLDTRKTIPGLRLAQKYAVHCGGGLNHRMGLYD
                     AFLIKENHIKACGSVAHAINLARKTDKHLLVEIEVETLDELQEALDAHPDRILLDNFT
                     QDMLEQAVKMNHPKYCELEASGGININNIADIAQTGVDFISVGSITKSIQAIDLSLLI
                     RETL"
     gene            complement(2806..4245)
                     /locus_tag="I9760_002182"
     CDS             complement(2806..4245)
                     /locus_tag="I9760_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAT5043945.1"
                     /translation="MSNSKWWFLLVCFIPAITFASEGGEHSDPVTSVLLGVTTILFLA
                     IIGRYLARLLNQPGVLGELLIGMIVGNLSYFFGSELVVILREGSAIYDVVKEMLAGIS
                     LNQAVTHVIHNSYYANQVIHALSGAEGTEYLKIAYVVDIFSRYGVIFLLFMVGLETSL
                     SDLKKTGKESILVAVIGIVAPMILGFVVACFIIPNSNYKINLFIGATLSATSIGITAS
                     VLKEMNKLSTREARTILGAATLDDILGLIILAVVSSIVISGAVSIMVVMKIIILALLF
                     FIGTLFMGPFVLRKAVDLFRFLEPWESKLFISFLFIMALAWLASFIQLAAIIGAFAAG
                     VILHDDYFGEQKAHHKDHRSIHHLVSPLETILAPMFFTLIGIQVKLETFYDWNVIVLA
                     GGLSVAAILGKLLSGLGANRRDDRLLIGIGMLPRGEVGLVFASIGRTIGVISDNLFTA
                     IVLMIMITTFIAPPLLKMRYARPKEDRNA"
     gene            complement(4487..6874)
                     /gene="ppsA"
                     /locus_tag="I9760_002183"
     CDS             complement(4487..6874)
                     /gene="ppsA"
                     /locus_tag="I9760_002183"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAT5043946.1"
                     /translation="MTVKRHTIDLAHLGMRDLDQVGGKNSSLGEMISHLSSAGVSVPG
                     GFATTADSFREFLSQNGLDKKIYDKLTTLDTDDVCQLAVVGKQIREMIIDTPFTPEFE
                     QSVRSSYQKLAQEIGHDNFSVAVRSSATAEDLPDASFAGQQETFLNVKGEEAVLAAIK
                     QVFASLFNDRAIAYRVHHNFAHNEVALSAGIQQMIRSDLAVSGVMFTMDTESGFDQVV
                     FITSSYGLGEMVVQGAVNPDEFYVHKPCLEAGKPAIIRRNLGSKALKMVYCDDPSLER
                     RVKTVDVDPAERLLFSLSKEEVEQLANQALIIEKHYGRPMDIEWAKDGVNGKLYILQA
                     RPETVKSRDNKQVLERYTLQKRGDVLAEGRSIGQRIGQGKAKVIKDINEMHRVQPGDV
                     LISDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATKTIKDGDV
                     VTVSCAEGDTGFVYSGLLPYEQERLDVETMPELPMKVMLNVGNPERAFAFQSIPNSGV
                     GLARLEFLISNTIGIHPKALLEFDTLKDEELKRYIKEKTIAYDSPVEYYIERLKEGIA
                     TIAAAFYPKPVIVRLSDFKSNEYANLVGGSLYEPQEENPMLGFRGASRYVSSNFSECF
                     ALECRAVRRVREEMGLDNVEVMIPFVRTVSEAGNVIEVLKKHGLERGKCGLRVIMMCE
                     LPSNALLASEFLQYFDGFSIGSNDLTQLTLGLDRDSGLVASQFDERNDAVKALLHMAI
                     AACKKEGKYIGICGQGPSDHQDFAQWLMKEGIESVSLNPDSVLQTCLFLAKQSMN"
     gene            7099..8097
                     /locus_tag="I9760_002184"
     CDS             7099..8097
                     /locus_tag="I9760_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAT5043947.1"
                     /translation="MFKRIGLISIALSLNTISLTSEACSALVFNKNKPATVAVNLDWK
                     YREGAFVIHPRNAMMVSSVDYHSMHPLIWKSQYGSVIFHGGNRFKAGPSADGMNEKGL
                     TASILMLDSSSYPSNSELPALKTTEWVQYILDNFQTVQEVIDDSSNYQLVADTYRGIT
                     MNVHLMVSDAEGKSAVFEYLDGKLVIHTQDNLTMPVLTNTDYKSSLALLEEYQDHGGS
                     KALPGGYDSKSRFVRAAHYLKKLPSFIAKEEHIAYAFNGLNVVTQAPGTSSPTQLSMV
                     FDIPTKTIYFRSINEAALRVISMDSIDFDYLYQPIALNVYQHLSGDVTDKFQPMIG"
     gene            8326..>10007
                     /locus_tag="I9760_002185"
     CDS             8326..>10007
                     /locus_tag="I9760_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT5043948.1"
                     /translation="MSIKNEYETARDHFRLWDKQLKDNILLEKSCLMHTYKTYFPDEP
                     GFIQQLSQFSKAVAEELEPTVMENNLDANLPKIEQYNAIGKRDDRVIHHPAYVRSGDI
                     IYGSNLMQYLLKPGQMKKTLSLFLLSSHAGEAGHNCPIACSAGVIRVLSRYSQLEKTS
                     YYLDKLTHPSFSYNFTGAQFLTEIQGGSDVGANATRANLDKQNQWRISGEKWFCSNAN
                     ADLILMTARFDEETPGTKGLGLFLVPSRLDDGSPNHYYLRRLKQKIGTRTMATAEIDF
                     EGALAYPMGDVAEGIHLVMENVLHLSRIFNSFSVLGMSRRAYQIACYYAKNRRAFNHP
                     IIEYPLVKESLAKIKSENLAMLASVFHMARCQDELDALPEPQQTKSQQLLLRTLANIN
                     KYFTAKRSVDNIHHCLDLLAGNGTIENFSSLPRLLRDCIVCENWEGTHFTLWMQTLRD
                     MHKFAIDELFIAHLQQLLEQVQDINPHKALFFEKINELDTTIKTMKSANHEEQSLHIK
                     TIIEHMASLLAALCLAIEIQQGQSPETKQAGLTLFIHDHIASCLLQHDDYMK"
BASE COUNT         3172 a         2240 c         1758 g         2837 t
ORIGIN      
        1 ttaattgctg tttgtcttga ataacgggtt tggcagaatc tatccatggg aattcgctat
       61 aaacttgttc acctagaatt tccgcacatt gtttcgcttg ttttattctc tcatcaggaa
      121 tatcacaata caactgaggt aattttatct ctccggtgtt ttcgtcctct atcctgctga
      181 tcaattgccg agctactctg aaactgtctg ccactatacc actggcactc cccgaatgaa
      241 ccccctcatt aattaattca acagttaact taccaaccaa attaccgcgt aatgacgtag
      301 tcatccataa ctgctcataa ttacctgctc cggaatcaag acaaataacc aatgatggtt
      361 taccaatacg ctcttttagc aactcaatat aaaaaggcaa atcgtaactg ccactttcct
      421 cacacgcctc gatgattaat atacaacgag gatatggcaa accttgctgt tccaatgcgc
      481 gaatagccgt aagtgatgca taagcagaat atccatcatc tgcccctcct cttccgtata
      541 acaatccatt tttcaatacg ggtttccatg gatgtaaatc gtcactccag cctgacatct
      601 caggttgctt atccaagtgc ccataaagca acacagtgtc atcaatttgg cctggaattt
      661 ccataaatag taatggagtc ctatttttca ggcgaacaat ttccagcgtc attcctttgg
      721 gagcatgcga cttacaccaa ttggcaatgt gattaactgc ctgctccata taaccatgtt
      781 cttcccattt tgcatcaaag tgaggcgatt tattagggat ttttatgtag tcacataaac
      841 ttggcaatat ctcttcttgc cactgttggc atatgtaatc atacaatcct tggggtttga
      901 acatgtgctt gctccttgat aattgccaca atcttatcag tggcagactc aatttttaat
      961 gctttgattt tattatctat ttcatctttt tttcgcatta cgtcctgtac tgcctgtaat
     1021 agaacatcag ctttcaataa ctcgtcctga atcacaacgc ttattcctaa tccctggaag
     1081 taccttgcat tctgaatttg atctcctctg cttacttgtg aagagattgg aattaagata
     1141 tgtggttttc ctaatgctaa tatttcatac aaagaattag ctcctgctcg agaaatcacc
     1201 acagaagaag cagcgaacag atcagccaac tcttcattgg cgtattcaaa ttggcaatat
     1261 ccctccgcgc caaccaacga agaatcaagt tttcctttgc cacaaagatg aataacttga
     1321 aattctgatg tcaattgttt caatgcgctt cgaatacaac tgttaattga accagcccct
     1381 aagcttcctc ccactacaag caggcaaggt ttggaggaat taaatccgca taactccaat
     1441 cctttcattc gatttccagt taatagctgt tgacgaattg gagtacccgt cacttctatt
     1501 ttatcctgac gcttaaagta ttttttgcca gcatcaaaag taaggcatat tttattgacg
     1561 aaaggaaagg ataggcgatt cgcaagtccc ggactcatat cagactcatg agcgacaaca
     1621 ggaattcgat ttaaccaggc gcctacaacc acaggaaagg ctacaaagcc accttttgaa
     1681 aaaaccacat cgggtttgat tttataaaat agcaaagaag attgaataat tcccagaaca
     1741 attttgaaag gatcaagcaa gttcttcaaa ctaaaatacc tgcgcaattt accactactg
     1801 actccatgaa aaggaatgtc cagcggctca atcatctcct tttcaattcc ggaaacagag
     1861 ccgatatatt ctacattcca gccttctttt cgaaattcct taatcaaagc gatattaggc
     1921 gttacatgtc cggcagtacc tcccccggta aaaacaatac ttgggctcat aatgtttccc
     1981 taatcaataa gctcaaatca attgcttgaa ttgatttggt gatggatcca actgaaataa
     2041 aatccactcc ggtttgagca atgtctgcaa tattattgat attaatgccg ccagaggctt
     2101 ccaattcaca gtacttggga tgattcattt tgactgcttg ctcaagcata tcttgagtga
     2161 aattatccaa taatatcctg tcagggtgag catccaaagc ctcttgcaac tcatcaaggg
     2221 tttccacttc aatctcaacc agtaagtgtt tatcggtttt ccttgccaga ttgatagcat
     2281 gagcaactga accacaggct ttgatatgat tttctttgat tagaaacgca tcataaagtc
     2341 ccatacgatg atttaacccg ccaccacaat ggacagcgta tttttgcgct aaccgaagtc
     2401 ctggaattgt tttgcgtgtg tctaacagct ttgccttggt accttttaat ttctgaacat
     2461 agtgataagt ttgtgttgca gtagctgaaa gggtttgtaa aaaatttaac gcagtacgct
     2521 ctgccgttaa aatactactg gcagtaccat gaataataca taaagtagcc ggcgtgctta
     2581 aaaaatctcc ttcagaaacc cgccactcca tttcaactgt attatcaact tgcttaaaaa
     2641 cctcatttac ccagggctgc ccacaaacaa gcataggttc tcttgatata atttctgctt
     2701 ctacaatcag ctgctgaggc aacaaagccg cagtaacatc accgttgccc acgtcctctt
     2761 gcaaagcacg ttttacatca aatgtaactt gcgataaaac attattcatg cattcctatc
     2821 ctcttttggt ctggcatatc gcatctttaa taaaggtggc gcaataaagg ttgtgatcat
     2881 gatcatcaat acaattgcgg taaacagatt atcacttatt acgcctatag ttctgccaat
     2941 agaagcaaaa accaaaccta cctctccacg aggcagcatt cctataccaa tgagcagcct
     3001 atcatcccgt cgattagcgc ctaagccact aagcagcttg ccaagaatag ccgctactga
     3061 taagcctccc gctaaaacaa tgacattcca atcataaaaa gtttctaact taacctgtat
     3121 cccaatgaga gtaaaaaaca taggagccaa tatagtttcc aagggagata ccaaatgatg
     3181 aatactgcga tgatctttat gatgggcttt ttgctctcca aaatagtcgt cgtgcaaaat
     3241 gacgcccgca gcaaaagcac caattatagc cgccaactgt ataaatgaag ccaaccaggc
     3301 taatgccata ataaaaagga atgagataaa taatttggat tcccaaggct ccagaaaacg
     3361 gaataaatcg actgctttcc gaagtacaaa aggccccata aacaaggtac caatgaaaaa
     3421 cagtagggct aaaatgataa ttttcattac aaccatgata cttacagcac cactgataac
     3481 gatactgctg actaccgcca aaataatcag gcctaaaata tcatccaaag tagccgcacc
     3541 aagaattgtt cttgcttcac gtgtactcag tttattcatc tctttaagaa cacttgctgt
     3601 aataccaatg ctggtagcac tcaaggtagc gccgataaat aaattaatct tgtaatttga
     3661 gttaggtatg ataaaacaag ccactacaaa ccctaaaatc attggcgcaa cgataccgat
     3721 cacagcaaca agaatggatt ctttgccagt ttttttcaaa tcagaaagcg acgtctcaag
     3781 accaaccata aaaagaagaa atatgacacc ataacgtgag aaaatatcaa cgacataggc
     3841 tattttaaga tattcagttc cctctgctcc acttaaggca tggatcacct ggttagcata
     3901 ataggaatta tggataacat gagttaccgc ctgatttaaa gaaattcctg ctaacatttc
     3961 tttgacaaca tcataaatgg cagaaccttc gcgaagaatt actactaatt cacttccgaa
     4021 aaagtaagag agattcccaa ctatcatccc tatcagtaat tcaccaagaa cgccaggttg
     4081 attaagaagt ctggctaaat aacgaccaat gatagctaaa aataaaatag tagtgacacc
     4141 cagaagaaca gaggtgactg gatctgaatg ctctcccccc tcagatgcaa aagtaatagc
     4201 aggaatgaaa cagactaaaa gaaaccacca ttttgaatta ctcataccgc tttatcctat
     4261 aagcttaatg tgctgctttc tcccaaatgc aagggaattt tgaattgcaa caccatacca
     4321 tcacaagtag ttccattact aacagccaaa ataattgtat tgatcaaaaa aactcatctt
     4381 ccaaggcaat ctgctgcatt tcaaaatcat aggaatgcct cagtaaattc ctaatgcacc
     4441 aacagcctaa caatttcaaa ctgaattgcc agtacactct ggttcatcaa ttcatcgatt
     4501 gtttagctaa aaacaaacat gtttgtaaaa cagagtcagg gtttagagaa acactctcaa
     4561 ttccttcctt catcagccat tgggcaaaat cctggtgatc tgaaggacct tgaccgcaaa
     4621 tacctatata cttgccttct tttttacaag ctgcaatagc catatgcaat aaagctttca
     4681 cagcatcgtt gcgctcatca aattgtgaag ccaccaaacc tgaatccctg tctaatccta
     4741 aagtcaattg agttaaatca ttcgatccta tggaaaaacc atcaaaatac tgcaaaaatt
     4801 cactggctaa caaggcattg gaaggtaact cgcacatcat aatcactctt aacccacatt
     4861 ttcctctttc cagaccatgc ttttttaata cttcgatgac attaccagct tcagaaactg
     4921 ttcggacaaa aggaatcatg acctcaacat tatctaaacc catctcctcc ctgactcgtc
     4981 ttactgctct acattcaaga gcaaagcact cagaaaaatt tgaagaaaca taacgagagg
     5041 caccacgaaa accaagcata ggattttctt cttggggttc atacaatgag ccaccaacca
     5101 gattagcgta ttcattcgat ttaaaatctg aaagcctgac tataacgggt ttgggataaa
     5161 aagcagcagc aatagtcgct atgccttctt ttaatctttc aatgtaatat tctacaggtg
     5221 aatcatacgc tatggtcttt tctttaatgt accgcttaag ttcctcatcc tttaaagtat
     5281 caaattccag aagcgcttta ggatgaatac ctatcgtgtt ggaaatcaaa aattcaagac
     5341 gggccaatcc cactccggaa ttaggtattg attgaaatgc gaaggctctt tcagggtttc
     5401 caacattcaa catcactttc atgggtaatt cgggcatggt ttctacatca agacgctctt
     5461 gctcgtaagg caacagacca gaatagacaa aacctgtatc gccttccgca cagcttacag
     5521 tcaccacatc accatcttta atcgtttttg ttgcatcacc acacccaaca actgcaggaa
     5581 tccccaattc acgagcaata atagctgcat gacaagttct gcctccacga ttggtaacga
     5641 ttgcagaagc acgcttcatg acaggttccc aatcaggatc ggtcatatca gatatcaaaa
     5701 catcgccggg ttgaaccctg tgcatttcat taatatcttt aataaccttt gctttaccct
     5761 gaccgattct ttgacctata ctacggcctt cagccaatac gtcgcctctt ttttgcaaag
     5821 tataccgttc gagcacttgt ttgttatccc tgctttttac agtctccgga cgagcttgca
     5881 ggatatacag tttaccattg accccgtctt tcgcccattc aatatccatg gggcgaccat
     5941 aatgtttttc aataataagt gcctgattcg ctaattgctc tacttcctct ttggataagg
     6001 aaaacaataa tctttcagca gggtctacat cgactgtttt aacacgtctt tcaagggaag
     6061 ggtcatcgca ataaaccatc tttaacgcct tgctgcccag gtttcgacgg ataatcgccg
     6121 gttttccggc ttcaaggcat ggtttatgga cataaaattc atctggattt acagcgcctt
     6181 gaacaaccat ttcacctaaa ccataagagg aagtgataaa tactacttga tcaaaaccgg
     6241 attcagtatc catagtaaac atgacaccac tgactgccaa atcactacga atcatttgct
     6301 gaatgccagc agacaatgcc acttcgttat gagcaaaatt atgatgcaca cgataagcta
     6361 ttgccctgtc attaaataaa gaagcaaata cctgtttaat ggcagccaat accgcctctt
     6421 ctcccttcac atttaaaaaa gtttcctgct gaccggcaaa agaagcatcg ggcaaatctt
     6481 cagcagtagc agaagatctt actgcaacac taaaattatc atgcccaatt tcttgagcaa
     6541 gtttctggta agaggagcga accgactgct caaactccgg ggtgaatggc gtatcaataa
     6601 tcatttccct aatttgtttc cctaccactg ccagttggca gacatcatcc gtatcgagag
     6661 tggtcaactt atcataaatt ttcttatcaa gaccgttttg ggacaaaaat tctctgaaag
     6721 aatcagcagt tgtagcaaat cctcccggca cagaaacgcc tgctgatgac aaatgactaa
     6781 tcatttcacc aagagaagag ttcttaccac cgacctgatc caagtcgcgc atgccaagat
     6841 gtgccaaatc tatagtatgt cttttaacag tcataaccgc cccaaattca taaaatagta
     6901 aacattctac ttaaattgtt actttattgg aattagaact tgtgctgtat ttcattttta
     6961 taaaggtcaa aataccggca ttttcacaaa aaatgacaag tatatgtact atgcggtaag
     7021 atatcttgct gtccatgttt tagggttgaa cgggtaagca aacctgttcc aagaatataa
     7081 taagcaaaag gatttgaaat gtttaaacgc ataggactaa tatcgattgc cttatcttta
     7141 aatactatct ccttaaccag cgaagcctgt tctgccttag tgttcaataa aaacaagccc
     7201 gcaactgttg cagtcaattt ggactggaaa taccgggaag gagcctttgt gattcatcct
     7261 cggaatgcca tgatggtttc cagtgtagat taccacagca tgcatccatt aatctggaaa
     7321 agccaatatg gaagtgtgat atttcacgga ggaaatcggt ttaaagcagg accatctgct
     7381 gatgggatga atgaaaaagg acttactgcc tcaatactga tgcttgattc atcaagttat
     7441 cccagtaact ctgagcttcc cgctttaaaa actactgaat gggttcagta tattttagat
     7501 aattttcaaa cggtccaaga agtcatagat gacagctcaa attatcaact ggttgcagac
     7561 acctaccggg gaataaccat gaacgttcac ttgatggtga gcgatgccga aggaaaatct
     7621 gctgtttttg agtatctgga tggtaaactg gtaattcata ctcaagacaa cttgaccatg
     7681 ccagtactaa ccaatacgga ctataaatcg tcattggctc ttcttgaaga ataccaggac
     7741 catggtggct ctaaggctct gccaggtgga tatgattcta aatcccgttt tgtaagagca
     7801 gcacattatc taaaaaaact tcccagcttt atagctaaag aagaacatat tgcttatgct
     7861 ttcaacggcc tcaatgtcgt cacacaagca cctggtactt cttctccaac ccaactgagt
     7921 atggtgtttg atatcccgac caaaacaatt tacttccgca gcattaacga ggctgcatta
     7981 agagtgattt caatggatag cattgacttt gattatcttt atcaaccaat cgccctgaat
     8041 gtttatcaac atttgtccgg ggacgtcact gataaatttc aacccatgat aggataaatt
     8101 ggaattcact aggccctctt gcagaacctg attattttgt ttaatgaaag aggcaagttt
     8161 aggcaagagg tctattgtga agccgcatgg atatggcaca aacgaagccg agggggataa
     8221 ttgattaata accgctatta caatttattg aaaaatattt aagtacaaca agaacttaaa
     8281 aaactgtgat atcaatgcat ctattagtta ccaaaggatg gggtaatgag cataaaaaat
     8341 gaatatgaaa cagcaagaga ccatttcagg ttatgggaca aacaactcaa agacaatata
     8401 ctgctcgaaa agtcttgttt aatgcacact tacaaaactt attttcccga tgagcctggt
     8461 tttattcaac aattgagtca attttccaaa gcagtagctg aagaattaga accaaccgtt
     8521 atggaaaata acctggatgc caacttgcca aaaatcgaac aatacaatgc aatcggtaag
     8581 cgagatgatc gggtaatcca ccatcctgcc tatgttcgat ccggcgatat tatttatgga
     8641 tccaatctca tgcaatattt gctaaagcca gggcaaatga aaaaaacgtt gagcctgttt
     8701 ctgctctcat cgcatgccgg ggaagcggga cacaactgcc ctattgcctg ttctgcggga
     8761 gtaatacgtg ttttaagtcg ttattcccaa ttagagaaaa cctcatatta tctcgataaa
     8821 ctcactcacc cttctttcag ttacaatttt actggagccc aatttttaac tgaaattcaa
     8881 ggtggttctg acgttggcgc caatgcgacc cgtgccaatc tggataagca aaatcaatgg
     8941 cgcatttcag gtgaaaaatg gttttgctcc aacgctaatg ccgatttaat tttaatgaca
     9001 gctcgtttcg acgaggaaac tcccggaacc aaaggattgg gtttgttttt agtgcccagt
     9061 cgcctggacg atggcagccc taatcactat taccttcgtc gattaaaaca aaaaattggt
     9121 acccgcacca tggcaaccgc agaaatagat tttgaagggg cgctagccta ccctatgggt
     9181 gatgtggcgg aaggaataca tttggttatg gaaaatgtgt tgcatttatc gaggatattc
     9241 aattccttct cagtcttggg tatgtctcgt cgcgcctatc agattgcttg ctattatgct
     9301 aaaaaccgcc gagcatttaa ccatcctatt attgaatatc ctctggtaaa agaaagtttg
     9361 gctaaaataa aatcagaaaa tctggctatg ttagccagcg tgtttcatat ggctcgttgt
     9421 caggatgaat tagatgctct tccagagcct caacaaacca aatcacaaca actcttatta
     9481 cgtacactag cgaatatcaa caagtacttt acagccaaac gttcagtgga caatatacat
     9541 cattgtctcg atttattggc aggaaatggc accatagaga acttttcttc attaccaaga
     9601 ctattaagag actgtatcgt ttgcgaaaat tgggagggga cgcattttac attatggatg
     9661 caaaccctca gagatatgca taaatttgca attgatgaat tattcattgc ccatttgcaa
     9721 caacttctgg aacaagttca ggatatcaac cctcataagg cattgttttt cgaaaagata
     9781 aatgagttag atacaacaat aaaaacaatg aaatcagcca atcatgaaga gcaaagtctg
     9841 catataaaaa caatcattga acatatggcc tcgttgcttg ctgctttatg tctggctata
     9901 gagatacaac aaggccagtc gccagaaaca aaacaagctg gattaacttt atttattcat
     9961 gatcatatag caagttgttt acttcaacac gacgactata tgaaaat
//
LOCUS       DACTJE010000119         9916 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.119, whole genome shotgun sequence.
ACCESSION   DACTJE010000119 DACTJE010000000
VERSION     DACTJE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9916
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.119"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            869..2371
                     /gene="ltrA"
                     /locus_tag="I9760_002186"
     CDS             869..2371
                     /gene="ltrA"
                     /locus_tag="I9760_002186"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAT5043949.1"
                     /translation="MQMTTSQTNDADAAPTTIKWQTINWLACHEEVKKLQRRIAKATR
                     EKRWRKVNSLQWLLTHSFSAKAIAVKRVTENKGKRTAGVDGKIWSTPEAKSKAITQLK
                     RRGYKPYPLKRVYIPKSNNTKRPLGIPVMRDRAMQALYLLALEPVSETTADWNSYGFR
                     PRRSTHDAISHLFVMLARKGAAQWVLEGDIKGCFDTISHEWILNNVMLDKRMLQHWLK
                     AGYIDKGHLFPTQEGTPQGGIISPTLANLVLDGLETLLATKFGSLKHDGHASRTSKYQ
                     VHFVRYADDFVITGKSKTLLEDEVKPLIKDFLAQRGLKLSEQKTKVTHITHGFDFLGQ
                     NIRKYRLGKANEKLLIKPSANNVKALKLKVKTMITKLCTAKQEEVIGVLNPIIRGWAN
                     YHHHIVAKETFTKVDHFIWRSLRCWACRRHPNKNLRWVKQRYYLAIENRRNMTFACRS
                     NETGKKAKLLTLCHAADTKIIRHNKIVGAANPYHPQYDDYFKKRSCERYL"
     gene            2607..3962
                     /locus_tag="I9760_002187"
     CDS             2607..3962
                     /locus_tag="I9760_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HAT5043950.1"
                     /translation="MDSINWHTFRFELEIEKNLDHRTPFEKDKCKVVHSYSFRRLQGK
                     IQIVSGKDSDFHRNRLTHSIEVASIAKSIFNNLLYRLQQDKKKNLEDKLIFLNSQKNE
                     DIDTLLDTIGLLHDIGHPPYGHGGEQALNYKMRNYGGFEGNAQTLRLINKVDSLNLTY
                     RTLLGVLKYPATYSALVNVKTYPNLDQRISDQKEITEYLIDNSRWNPPKCYYDSDNPT
                     VLNLLSYITDNERIEFQRSFPAPKPDHKKTKYKTFDCSVMNIADDISYSIHDLEDAIF
                     FRQISMDELVNFFENQNFIQNWSNYPTLLLSDDLKARKKVIGTLIHEAIIHTCIEENS
                     NFNEPIFRYFVKFENEAIETFIKNLKLLVSNNLIKTQHVKSIIHGGKVLIMQLFDAID
                     VNPEMLLPTEVFQKYSRQTQEADQKRIIADYISGMTDNYLYKMHQRIFGGNTQSTFDT
                     I"
     gene            4546..4797
                     /locus_tag="I9760_002188"
     CDS             4546..4797
                     /locus_tag="I9760_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT5043951.1"
                     /translation="MSKNHVSRLQLINEFESAPESTLFNQNTLAAVLDCSVQLLERNR
                     WAGQGVPYLKIGRTVRYRKLDILNYLQHQCVHNTHQEAV"
     gene            4862..6571
                     /locus_tag="I9760_002189"
     CDS             4862..6571
                     /locus_tag="I9760_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAT5043952.1"
                     /translation="MNSEQIYHVDFKQSKRIKSFHYAGGLFQLTSDGVIFVGKDKDNN
                     ELPPRWICSSLSVVAKTRDANSGEWGRLLEWVDDDGIKHQWAMPLALLQGDSSDVRRE
                     LARLGLTISPSKTARDLLASYIQVFPVEERARCVDKLGWYKEVFVTANEAIGQSLSVA
                     GSVEDWRNSISRLADGNSRLVFAISAAFAPSLANLVGEDSGGFHFRGASSSGKTTALK
                     VAASVWGKPDSYIRLWRSTANGLEGLAALHNDGLLILNELSQMDPKEAGECAYLLANG
                     QGKTRASRCGTARQSMRWSLLFLSAGEESLTSLMAKAGQRCNAGQEIRLADIEADAGA
                     EMGLFEQLHDHINPAAMSLALKEAASQYHGAVGLAWLHKIVKHRAELIALLTNKIQHF
                     VAKVTKPEHSGQIQRVARRFALVAMAGEIATHYELTGWTRGTACNPAEKCFNAWLKDF
                     GEHGNREDRAILSQVRAFFEKEGASRFENENHPNSERLFNRAGFFHTDSEGFRVFMVL
                     SEVYRKEICQGFEPKMVNKVLINAGWIVPGNDGKASQKRRIKGIGIPRCYIFTEKVWS
                     DDY"
     gene            complement(6768..7109)
                     /locus_tag="I9760_002190"
     CDS             complement(6768..7109)
                     /locus_tag="I9760_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HAT5043953.1"
                     /translation="MEEYYDNGVSDNLLIKLNEILVNVNIQCLFENIENRLVAVHVAY
                     MPYNPPPLAFGAYMFSNVTSLCGLEGLKRCKSNDCLKFFIGRRNAKWCSNSCGSKYRV
                     NKLRKNKRAVY"
     gene            7360..8544
                     /locus_tag="I9760_002191"
     CDS             7360..8544
                     /locus_tag="I9760_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HAT5043954.1"
                     /translation="MSKRVTTADFIRRARDVHGNRYDYTKVFYVAARSKVVIICPEHG
                     EFKQIPAGHLSGRGCHECGGNKPLTIARFIERASKKHGNHYDYSHVQFQNVESKIEII
                     CPDHGLFFQQLMSHLKGFGCPQCGRINTANKLSHSIQRFIEDAKRAHGNKYDYSQVRY
                     VNALTKVTIICPDHGPFDQKPSNHIRDVGCPKCGDESAAMIRTRTTEEFVQEAKEVHG
                     ALYDYSKVDYKSSHKHVEIICLVHGSFWQSPVNHVRGNKSGCPGCAVSGFDQTKPGLL
                     YYIAVKKDDGELLYKIGITNLSIERRFPSVDRARIRVIRIWRFELGQIAAEYEANIIS
                     QFNRYRYYGPAVLMGSGNTELFTKDILSLDKSHHDLRDAVVDLEGNLISRPRQLEFDL
                     SE"
     gene            complement(8662..9765)
                     /locus_tag="I9760_002192"
     CDS             complement(8662..9765)
                     /locus_tag="I9760_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4263 domain-containing protein"
                     /protein_id="HAT5043955.1"
                     /translation="MGTITYSYKSTSSSSAEIEDIEFSRTSTTRRIIRPMIVENNERP
                     DASIKIEIIHQKAEKGLFEDIPTEKLSKYKAKDIGKLVLSSEETLSLFNHLCNLYLIH
                     KENGIPSGYHEFEVDENSRLMSIDSKRAAIIETLIQQGYSEEVWEKLVENSPDLATKL
                     SIARLYQTRMTALEQFRTMLSCDHSEQDWQSFFENNNWIFGYGLNYKILKAITGQPHY
                     GGVVIDGSGTNKGDFLSATQGYLKFTVLVEIKRSDTKLLASKRYRNDVVLPSEDLIGG
                     VSQLRVNGRTWEQQGSQLPKNRDLLEDDSIYTIQPRKILVIGNTNELNDRLKRENFEL
                     FRRGQSDIEILTFDELLNRAQFITEHTEAIKDG"
BASE COUNT         3148 a         1821 c         1950 g         2997 t
ORIGIN      
        1 aaagaaccaa aacctagttc aaaatattta cgcaggaaat cgtcggtaaa ttcattctta
       61 ttaatcgtag taatttgatc aattagcgcc attttattta tcctttattg gttaatccaa
      121 aatccctttt aatgacatta ttcttcatta aatattagac taaagaatat atttttggtt
      181 taaatttcaa cattgcaagg caatgtatat tgcgccctta ggtgcgactc gatgggtcgt
      241 ctatgtgatt gtacgggtag tcaaactacg gaatgcattt attggtttcg ttacgggact
      301 attagagtac ctatgattcc gttcatagta gcctaattcg acgtgcgtca ttggtaacga
      361 tggcgtgcga agctcgaatg acaatgtact gaagtttacg catgtaaacg tcgtggattt
      421 gctgataggt aaacacgaat ctggaagctt catccggaat aaggctaggc taatgggtat
      481 ggttaatgta agtgaactgc tgataaacat cgaaagcaat gatgagctaa agaagctgat
      541 aagcgaaagc caaaatggca tgcatgggac aatccaggtt tgaggttgga ttgcacggac
      601 aaacaacatg cgcggtgaag aggcggaacc taaccccaat tttgtcatgt agacggaacg
      661 aggtaaaccc gactcagtgc cttagcaatt ttaagtaagg caggttaacc gcaaggaaaa
      721 ctgatgctga agcgggcaga agatagacta aaaagcaaac gctcttctgt aatggaaaag
      781 ataggaacga ctgttctatg gtgaaagcca gcccacttgg tcttggtcaa cattgcgaga
      841 gactagcaga atttcttttt aaggagacat gcaaatgact acatcacaaa ctaacgatgc
      901 ggatgcggct cctactacta tcaaatggca aaccattaac tggttagcat gtcatgaaga
      961 agttaagaaa ctgcaacgac gtatcgcaaa ggcaacacga gagaaacgct ggcgtaaagt
     1021 gaactcatta caatggctac tcactcattc cttctcggcc aaagcaattg ccgtaaagcg
     1081 agttacagaa aataaaggca aaagaacggc cggtgttgat gggaaaatat ggtcaacacc
     1141 tgaagcaaaa tcaaaagcca tcactcaact taaacggcgg ggatataaac cctacccttt
     1201 aaagcgagtg tatatcccaa aatcaaataa cactaaaaga ccacttggca tcccggttat
     1261 gcgagacaga gcgatgcaag ctttatactt acttgcacta gagcctgttt cagaaaccac
     1321 tgctgattgg aattcttatg gatttcgccc acggcgttct actcatgacg ctattagcca
     1381 tctctttgtg atgcttgctc gcaaaggtgc cgcacaatgg gtactagaag gcgacatcaa
     1441 agggtgtttt gatacaatca gccacgagtg gattcttaat aacgtgatgt tagataagag
     1501 aatgctccag cactggttaa aagcgggata tattgataaa ggtcacttgt ttcctaccca
     1561 ggaaggcacg ccacaaggag gtattatctc tccaacgttg gcaaatttgg tattggatgg
     1621 tttagagacg ttgcttgcaa cgaaatttgg atcattaaaa cacgatggac acgctagccg
     1681 taccagtaaa taccaagtgc atttcgtgag atatgctgat gattttgtca tcacaggaaa
     1741 atcaaaaact cttcttgaag acgaagtcaa accgctaata aaggattttc tagcacaacg
     1801 aggtctaaag ctttctgagc aaaagactaa agtgacgcat atcacacacg ggttcgattt
     1861 tcttggtcaa aatatacgga aatatagatt gggaaaagcc aacgaaaaac tattgataaa
     1921 accatcagcg aacaatgtta aggcacttaa gctgaaagtg aaaacgatga taacaaaact
     1981 ttgtactgcc aaacaagaag aagtaatagg cgtacttaat cctataatcc gaggttgggc
     2041 aaattatcat catcacatag tggctaaaga aacatttaca aaagtagacc actttatctg
     2101 gcgatcatta aggtgctggg catgcaggcg acatcctaac aaaaacttaa gatgggttaa
     2161 gcaacggtat tacctagcaa tagaaaaccg tcgcaacatg accttcgcct gtaggagcaa
     2221 tgaaactggg aaaaaagcaa agctgttaac tctttgccat gcagcggata ctaaaattat
     2281 tcgccataat aaaatagttg gagctgctaa tccctaccat ccccaatatg atgattactt
     2341 taaaaaacgt tcttgcgaaa gatacctgta gtgttaccgg ctgatcctat gggattggct
     2401 tagaaaagaa acagacatct tctgtttcct tgattgaagc cgtatgcgat gaaagtcgca
     2461 cgtacggttt ttatggggga atgtcacagt aatgtgatgt tcctacctac taaaattctt
     2521 ttcttcggga cattgagtaa tttaatcgaa aactaaggaa aaaaacttcg tgtgctataa
     2581 ttttatttaa tcaaggatgg ttaatcatgg atagtatcaa ctggcacaca tttaggtttg
     2641 aactggaaat agaaaaaaac ttagatcacc gaactccgtt tgaaaaagat aaatgtaaag
     2701 ttgttcattc ttattctttt cgccggcttc aaggtaaaat tcaaattgtg agcggtaaag
     2761 attctgactt tcatagaaat agattaactc attccataga agtcgcctcg attgcaaaaa
     2821 gcatttttaa taatctatta tatcgattac aacaagataa gaaaaaaaat ctagaagata
     2881 agcttatttt cttaaactct cagaaaaatg aagatataga cactttgctc gatacaattg
     2941 gattacttca tgacattgga cacccaccat atggtcatgg tggtgagcaa gcactaaatt
     3001 ataaaatgag aaactatgga ggatttgagg gaaatgcgca aactttaaga ctaattaata
     3061 aagtagattc tttaaatctt acttatagaa ctttattggg agtactaaag tatcctgcaa
     3121 cctacagtgc attggtgaat gtaaaaacat atcctaatct agatcaaaga atctccgatc
     3181 aaaaagagat tacggaatat ctaatagata attcaagatg gaatcctcca aagtgttatt
     3241 atgatagtga caacccaaca gtcctgaatc tattaagtta tattactgat aatgaaagaa
     3301 tagagtttca aagatctttt ccagccccaa aaccagatca taaaaaaaca aaatataaaa
     3361 catttgattg cagtgttatg aatattgctg atgatatttc ttacagtatt catgatctag
     3421 aagatgctat tttctttcgg caaattagta tggatgaatt ggttaatttc tttgaaaatc
     3481 aaaacttcat tcaaaattgg agtaattatc caacacttct tctttcagat gatttaaaag
     3541 ccagaaaaaa agttatcggg acattaattc acgaagcaat aattcatact tgtattgagg
     3601 aaaatagtaa tttcaatgaa cctatattta gatattttgt aaagtttgaa aatgaggcta
     3661 ttgaaacatt tataaaaaac ctaaaactac ttgtaagcaa taatttaatt aaaactcaac
     3721 acgtaaagtc aattattcat ggaggaaagg tactaattat gcagctattt gatgcaattg
     3781 atgttaatcc tgaaatgtta ttaccgacag aagtatttca aaaatatagc agacagaccc
     3841 aagaagctga ccaaaaaagg attatcgctg attatatcag tggcatgacg gacaattatc
     3901 tctataaaat gcaccaaaga atttttggtg gaaacactca aagtacattt gacactatct
     3961 aattgaccga cctaaatcta ctggagttga acgaattatc tcatgcatca tattccaaat
     4021 catcaatcat tgatttaaaa gatgaatatt tctttccccc gacactctcc agctcctcta
     4081 tagcttcaag ggttagatca ttgggtttct cactatcctg aggattagga ttaatttctg
     4141 ataatgtttt catatcaaaa ttatactcca tttcccctat attgagagca gccttaatga
     4201 tttttagtgg tacgagtgga actaagggga catcttgata ttactaggcc ataagcgtgc
     4261 caccctcaaa atatcccatg gtacgcgtgg gacaaataac tcatatttgt tcactctaat
     4321 tacactttct ttgaatttgt ttccaattgt tgactttaat tttcaaacca cgtaataatc
     4381 tgttcgttgt tttaaatagc tttatgaaaa tggctttaaa gctatatgga tttttggata
     4441 gaaagtacca gtgctactgg aattttcaaa ttggatttat ggaaaaagcc tctttggcga
     4501 cattttttaa tgtcgaatga aaattctttt gagaaaggta ttactatgtc taaaaatcat
     4561 gtatcgcgct tgcaactcat caatgaattc gaatctgctc cagaatctac cctctttaat
     4621 caaaatacac ttgccgcagt tttagattgc tcagttcaat tacttgaacg taatcgttgg
     4681 gctggtcagg gtgtgcccta cttaaaaatt ggtcgcaccg ttcgctatcg caaattggat
     4741 attcttaatt atcttcaaca ccaatgtgtt cataacaccc atcaagaagc ggtttagcct
     4801 ttttttgaat aattggaagg tggtagctca ccttcctgcg acagttgttg gagagaataa
     4861 aatgaattca gaacaaatat atcatgttga ttttaaacag tctaaacgca tcaaatcgtt
     4921 tcactatgca ggtggactat tccagttaac ctcagatggg gtaatttttg ttggtaagga
     4981 taaagacaat aatgagctac ctcctcgatg gatttgctct tcgttgtctg tggtcgctaa
     5041 aactcgtgat gccaatagtg gcgaatgggg tcgattactt gagtgggtgg atgatgatgg
     5101 gattaagcat caatgggcta tgcctttggc tttattgcaa ggagactcca gcgatgtaag
     5161 acgggagctg gcgcgattgg gattgaccat ttcacccagt aaaacagctc gtgatttatt
     5221 agcttcctat atccaagtct ttccagtcga agagcgtgct cgttgtgtcg ataaactggg
     5281 ctggtataaa gaagtctttg ttaccgctaa tgaagccata gggcaatccc tgtctgttgc
     5341 aggtagtgtt gaggattggc gaaactctat ctcacgatta gcagatggta attctcgctt
     5401 ggtatttgct atttccgcag cctttgctcc aagccttgcc aatcttgttg gtgaagattc
     5461 aggtggcttt catttcagag gagcttcctc ttctggtaaa acaaccgctt taaaagtagc
     5521 agcttctgtt tggggtaaac cagatagtta tattcgttta tggcgaagca cagcgaatgg
     5581 gttagaagga ttggcagcac ttcataatga tggattatta atcctcaatg agctgagcca
     5641 gatggatcct aaagaagctg gagaatgtgc ctatctactt gcaaatggtc aaggtaaaac
     5701 aagagcttct cgttgtggta ccgcaagaca gtccatgcgt tggtcattac ttttcttatc
     5761 agctggtgag gagtcattaa cttcattaat ggcaaaagct gggcagcgat gcaatgcagg
     5821 tcaagaaatt cggttagcag atattgaagc ggatgctggt gcagagatgg ggttatttga
     5881 gcagttacat gatcatataa acccagccgc tatgtcactc gcattaaaag aagctgccag
     5941 tcaatatcat ggtgctgttg gtctagcatg gctccataaa attgtgaaac atcgcgccga
     6001 attgatcgcc ctactcacta ataaaattca gcattttgtt gctaaagtca ctaaaccaga
     6061 acattctggt caaattcaac gagtagccag acgctttgcg ttggttgcca tggctggaga
     6121 aatagcgact cattacgaac taactggatg gacacgtggc acagcatgta atccggcaga
     6181 aaaatgcttt aacgcttggc taaaagattt tggtgagcat ggtaatagag aagaccgagc
     6241 tatactgtca caagttcgtg cgttctttga gaaagaagga gcgagccggt ttgaaaacga
     6301 aaatcatccc aacagtgaac gcctttttaa tcgggctggc ttttttcaca cggacagcga
     6361 gggctttcgt gtttttatgg ttttatctga agtgtacagg aaagagatct gtcaaggatt
     6421 tgagcctaaa atggtgaata aagttcttat taatgctggc tggatagtcc ccggtaatga
     6481 cggcaaagca tctcaaaaac gaagaattaa agggattggt attcctagat gttatatttt
     6541 cactgagaaa gtttggtctg acgactatta attgtcccac tcgttccact gtcgattttg
     6601 atagtggaac atctaaagtc cagtaactac tggggtgttc cacttgtccc agtagttcca
     6661 cgtatttttt agatgtaggt aaaaatctat taggttgtat cgactttaat gaagatgatt
     6721 tcccatacta caactaaatg taatacggga aaatcattgt accttgttca atacacagca
     6781 cgcttgttct ttcgcagttt atttactctg tatttagagc cacacgagtt tgagcaccat
     6841 ttggcattcc tgcggcctat aaaaaactta aggcagtcat tacttttaca acgtttcaat
     6901 ccttcaagtc cacaaagtga agtaacattc gagaacatat atgccccgaa agctaatggt
     6961 ggtggattat atggcatata agcaacatgc actgccacca gcctattttc tatgttttca
     7021 aaaaggcatt gaatgttaac attcacgagg atttcgttaa gtttgattaa taagttatca
     7081 gataccccat tgtcataata ctcttccatg cactcataaa taaaagcctc taaggctggt
     7141 atggtacttt tagaattaat accgttttta ctgtctaaat gaatgttagc tgcttctatt
     7201 aaagtagaag cttcgttact gctgtatagc ttaagttctt ttcctgtatt gatataaaaa
     7261 tagtcatgta acgctttttt caatttaaat ccctattgaa gtcgtaacgg ctttgatgtt
     7321 tatatctcgt aacaatatca ttttcaaggt tgtgagaaaa tgtccaaacg tgttactaca
     7381 gcagatttca ttcgacgtgc tcgtgacgtg cacggcaatc gctacgatta cacaaaagtg
     7441 ttttatgttg cagctagaag taaggtcgta ataatttgtc ctgagcatgg tgaatttaag
     7501 caaatacctg caggtcatct ctccggtcgc ggttgccatg aatgcggtgg aaataagcca
     7561 ctgaccattg caagatttat cgagcgtgca agtaagaagc acggaaacca ttatgactat
     7621 tcccatgtac aatttcaaaa cgttgaaagt aagattgaaa ttatatgtcc ggatcacggc
     7681 ttgttttttc agcaattaat gtcacatcta aagggttttg gttgccctca gtgcggtaga
     7741 attaatacag ctaataaact gagtcattcc atccaacgtt tcattgaaga tgcgaaaaga
     7801 gcacatggga ataagtatga ctattctcag gtacggtacg tcaatgcatt aaccaaggta
     7861 actatcatct gcccagacca tggtccattc gaccaaaagc cgtccaatca tataagggat
     7921 gtaggatgtc caaagtgcgg cgatgaaagt gcggcaatga taaggactcg aacaactgaa
     7981 gagttcgttc aggaagcaaa ggaggtgcat ggagctcttt atgactattc aaaggttgat
     8041 tacaaatcat ctcacaagca tgttgaaata atatgcctag tacatggttc gttttggcaa
     8101 tcccctgtta atcatgtaag gggtaataaa tctggttgtc caggttgtgc ggtgtcaggt
     8161 tttgatcaaa caaagcccgg tttgttgtat tacattgctg taaaaaaaga cgatggtgaa
     8221 cttctataca agattggtat aacaaacctc tccatagaac gtcgatttcc ctccgtagat
     8281 cgtgctcgaa taagggttat aaggatctgg cgatttgaat taggtcaaat tgcggctgag
     8341 tatgaagcaa atattattag ccaatttaat cgatatagat attatggtcc tgcagtatta
     8401 atgggaagtg gaaataccga attgttcacc aaagacatac taagtttaga caagagtcat
     8461 catgatttaa gagatgcggt tgttgatttg gaaggcaatt tgatttctcg accaagacag
     8521 ttagaatttg atttatcaga gtaataaaac acagagatga ggcatttccc ccacaatgaa
     8581 tctattagac atcatagatg ctggagaaaa tgactcaaat tactcctgct taagtgcatc
     8641 attaatttcc ttcaatgaat actagccatc tttgattgcc tcagtgtgtt ctgttatgaa
     8701 ttgtgcccga tttaacagtt catcgaaggt taggatttca atatcacttt gtcctctacg
     8761 aaataactca aagttttcac gctttaatcg atcgtttaat tcattagtat tgccgataac
     8821 caaaattttt cgtggctgaa tagtataaat agagtcatct tctaggagat ctctattttt
     8881 tggtagttga gatccttgct gctcccaggt tctaccattc actcttaatt gagaaacccc
     8941 tcctattaaa tcttcactag gtaataccac atcatttcta tatctttttg aggctaatag
     9001 cttggtgtct gatcttttaa tttcaactaa taccgtaaat tttaaatatc cttgagtagc
     9061 acttaagaaa tctcccttat tagttccact tccatcaatt actacgcctc cataatgagg
     9121 ttgtcctgta atggctttta aaattttata attcaatcca taaccaaata tccaattatt
     9181 attctcaaaa aaggattgcc aatcttgctc actatgatca caggatagca ttgttctaaa
     9241 ttgctctaag gcagtcatgc gtgtttgata taaccgagct atactaagtt tagtcgctaa
     9301 gtcaggagaa ttttcaacca atttttccca tacttcttct gagtatccct gctgaattag
     9361 tgtttcaata atagctgctc ttttcgagtc tattgacatt aatctactat tttcatcaac
     9421 ttcaaattca tgatagccag acggaattcc attttcttta tgaattaagt ataggttgca
     9481 aagatgatta aaaagagaca atgtctcctc actacttaga actaacttac caatatcttt
     9541 agccttatac ttagacaatt tttcagttgg aatatcttcg aacaatcctt tttctgcttt
     9601 ttgatgaata atctcaattt tgatactagc atctggtcgc tcattattct caacaatcat
     9661 aggtcgaatt attcttcgtg ttgttgatgt tctactaaac tcaatatcct caatttctgc
     9721 tgaggagctc gaggtagatt tataactata agtaatcgtc cccattatta ccctttgttt
     9781 tttagacaga tattaggctc taccccaaca aagggggcac tttaatcaag ttagtataaa
     9841 ctaacctctt gatttcgcga tcaaaaagga gattaaagtg ccgagacatg atgttatcct
     9901 aaatttacct ggtttt
//
LOCUS       DACTJE010000120         9910 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.120, whole genome shotgun sequence.
ACCESSION   DACTJE010000120 DACTJE010000000
VERSION     DACTJE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.120"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..901
                     /locus_tag="I9760_002193"
     CDS             <1..901
                     /locus_tag="I9760_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043956.1"
                     /translation="EDIDFTIHSFGHFPVFPKYVDKPFYLGEDLPVVLAGGDCQIEPD
                     YRKGIGIESGIERANFLFDTVHGTSKGLGFLFDNYYQQVARYVGYHGNLIEQFYLQRV
                     DNIKGSSLEQAKKILCSACGSVKEIEDVAAIASELKLLGNELFKKPNYESALECYLNA
                     IHLYQSFEKALPLTMDFVTLHSNACQTCLKLKKYEQCINLANEGIKAYAEIKAEDKEM
                     LFKLLFRKASALVELGNAFDVKTQRKEFDEALKDLKETYELMQENSGVNNTAFVKQIQ
                     TKIVTIEKKLPPPQEEINKIEFI"
     gene            1067..2266
                     /locus_tag="I9760_002194"
     CDS             1067..2266
                     /locus_tag="I9760_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HAT5043957.1"
                     /translation="MLNKGYIKTTPYGLGFSELFFEDNLKKYFSDPSLEVLSAEYSPS
                     ASDKMAWSAKHPKQGDHPIGVQRYKISFKKNGKIQTRHVIVKSKISDKHYLQVISNAF
                     TKCGIQTEMPILDYLSQLEFSNLNRKEITIYNMQHSFEAFQYAMPLCYGTYLDEQEEA
                     CVLILECLDEEWLSLDPFNTSKWDQTAIEALIDAIAPMHAVWYKKEKTIAEIKGFKNP
                     LNTDQMQRFIPYWNALEKAVKTSNLPFLTKEDYLFHTHLIETMPQWHIKIDAMAKTLV
                     QNDCVPKNIALCNQNGTKKVLVYDWEIATIHIPQRDIVEFLSYVLPENFDSTLLGNYI
                     ERHRIKLGETTKQAIDATEWRLGFQYALYDYLIQRVFPQIPFEKLEARNIAKVYNNAR
                     RMMALLD"
     gene            2286..3023
                     /locus_tag="I9760_002195"
     CDS             2286..3023
                     /locus_tag="I9760_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HAT5043958.1"
                     /translation="MTHIRVILPGYNTKVLNKDMLKPFENPGLELSVDYADLAVDAVR
                     HEFDIALMAPYLIKKVIKAEQEKVHAIVIDMMADVAMRALREAVTIPVVSLAETATHV
                     AAMMGNNFAIINTTDELTPIQESLVRSYGLYDRLACIQGIQLDPHDDYRSEKIIHGLV
                     EQCADAVIKKRADTLIFGSGRLIGYREVIQKGLEEKGLHITIIEPLPTGIYFAKFLVD
                     SKISQSKYVYRSPDASTLKTYSKILGN"
     gene            complement(3063..4376)
                     /locus_tag="I9760_002196"
     CDS             complement(3063..4376)
                     /locus_tag="I9760_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAT5043959.1"
                     /translation="MNSIPHLILGDIITPTQAGQSLLIKKGYVLINGDSIIEVGEQAH
                     FNKEISYTVSNYPDHLILPGLIDTHSHLSQYAICGAGDLPLMDWLNTLVFPAETAFSS
                     NLQRCQRHAELYMHACLGSGTTTINTMVTSSRQATEIVCDVAARLGIRAFIGLVLMDR
                     NAPDNLLVDCAQAFKDLTVLKDRYHGKNNIHITVSPRFAVTCSAAMLRQAGEFARANK
                     LILHTHLDKDEGFDELIQSLFSTAHDYFDVFESTQCIADKTVFAHGTLLSLHEMKRMG
                     DYAKQVGISHCPSSNFSFAMGMAPVSFFKGLGIEVGLGSDSGGGDSLSLFDEMRSASF
                     TNKALWRLDKKTALIDAKTWLYHATLGGAKLLGLEKQIGSIEAGKKADLIILDDRNNY
                     PLAELSSLSQELALDELQYRLARVIARSCENKLVAVYIDGKKVYQ"
     gene            complement(4545..7397)
                     /locus_tag="I9760_002197"
                     /pseudo
     CDS             complement(4545..7397)
                     /locus_tag="I9760_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947702.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1986 family Dot/Icm T4SS effector"
     gene            complement(7574..8041)
                     /gene="sixA"
                     /locus_tag="I9760_002198"
     CDS             complement(7574..8041)
                     /gene="sixA"
                     /locus_tag="I9760_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAT5043960.1"
                     /translation="MKIYLVQHGDSLDKDIDPERPLSDQGQKDIQLLSNFLSLLKIEV
                     SCVYHSGKLRAQQTAESLSGHIFSKNGVESRAGLEPLDDVIPLAKEINQLKQNMMFVG
                     HLPFMNKLVSQLVVEEEHRILVSFKPGSLVCLDKADGIDWCIAWMICPELLRE"
     gene            8116..8661
                     /locus_tag="I9760_002199"
     CDS             8116..8661
                     /locus_tag="I9760_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAT5043961.1"
                     /translation="MKPKISVFIATSLDGYIARNDGSIDWLIEANSLASPGEDCGYRS
                     FIATIDTIIMGRYSYEKVITFDEWPYASIPVIVLSSKPIQIPEHLRHIISVSNQSPIE
                     LASELAHYGIQHVYLDGGITIQRFLQNNLIDEITITMIPVLIGSGRPLFGFLENDIKL
                     KHLSTTSYPGGFVQIKYHIGE"
     gene            8760..9176
                     /locus_tag="I9760_002200"
     CDS             8760..9176
                     /locus_tag="I9760_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAT5043962.1"
                     /translation="MDIRYLIYAWGSSILFFIYLYLSYLVIIQRRKYHVLYLTNDDKA
                     FIARFRAHANFAEYVPFTLILMGISIILKIPPLLLTVLMFSLIIARLGHAYGLIKKEA
                     EKQFKPRIYSVATTFIIMGILALFNIFKATILYIAI"
     gene            complement(9354..>9910)
                     /locus_tag="I9760_002201"
     CDS             complement(9354..>9910)
                     /locus_tag="I9760_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043963.1"
                     /translation="KKEFEKDLNHIESSYSELLKDKDFNSALQVEMKELEFRLKYVLF
                     NWFFLFKKDKQGLNKEICQFKVDILRLKTKFMISHVLLNPDKYNLEHYKLVVKHLHNQ
                     LEDLNDKIGLMKGIEIDIYSDLPSAMYEWFEQTYSLLSNRYDEFKDELSIGDVWEDWS
                     YDNCSSGDDEEYDEPVISFNNTIP"
BASE COUNT         3092 a         1979 c         1714 g         3125 t
ORIGIN      
        1 tgaagacatt gattttacaa ttcattcgtt tggacatttc cctgtttttc ccaagtatgt
       61 cgataagcca ttttatcttg gtgaagactt acctgttgtt ctggcaggcg gtgattgtca
      121 aattgaaccc gattatcgta aaggaattgg catagaaagt ggaattgaac gtgccaattt
      181 tctatttgat actgtacatg gtacaagtaa aggactgggg ttcttatttg ataattacta
      241 tcaacaagta gctagatatg taggctatca tggcaacctg attgaacagt tttatctgca
      301 aagagtggat aacattaaag gctcttcgct tgagcaagca aagaaaattc tttgctctgc
      361 ttgcgggtca gttaaagaaa tcgaagatgt tgccgccatt gctagcgaat taaagctttt
      421 aggcaatgaa cttttcaaga aacctaatta tgaaagtgct ttggaatgct atcttaatgc
      481 tatccatttg tatcaatcct ttgaaaaagc cttgccactg acaatggatt ttgtgacatt
      541 gcattccaat gcctgccaaa cctgcctgaa gttaaaaaaa tatgaacaat gcataaatct
      601 ggcaaatgaa ggcatcaagg cttatgcgga aataaaagca gaggataaag agatgctttt
      661 caaattatta ttccgaaaag cctcagccct cgttgaacta ggtaatgctt ttgatgtcaa
      721 gacacaaaga aaagagttcg atgaggcatt gaaagatctg aaagaaacat atgagctaat
      781 gcaggaaaac tcaggtgtga ataataccgc ttttgttaag cagattcaga ccaaaattgt
      841 aactatcgag aaaaaacttc ctcctccaca agaggaaata aataaaatcg aatttattta
      901 gctttaaatt ggtaacatta taaaaatgcc tggcttgctc attaatggat agaaatctat
      961 ctgacaatga acaagcctaa tcattatgac tctcttcccc aataaatgtt caataaagct
     1021 caatgctgct attcttaatt gaaatagggt tgataaggga gtgaaaatgc taaacaaggg
     1081 atatattaaa acaacaccct atggattggg attttcagaa ctttttttcg aagacaactt
     1141 aaaaaaatat ttttctgatc cttcactaga agtattgtca gcagaatact ctccttctgc
     1201 cagtgataaa atggcatggt ctgccaaaca ccccaaacag ggggatcacc ccataggagt
     1261 acaacgttat aaaataagct ttaaaaaaaa cggtaaaata caaacccgcc atgtcatcgt
     1321 caaatctaaa attagcgata agcattatct ccaggttata tccaatgcct ttacaaaatg
     1381 cggtattcaa acggaaatgc ctatccttga ctatttatca caactggaat tttcaaattt
     1441 aaacaggaaa gaaatcacta tttacaatat gcaacattca tttgaagcct ttcaatatgc
     1501 tatgccatta tgctatggaa cttatttaga cgagcaagaa gaagcttgtg tattaatttt
     1561 agagtgcctt gatgaagaat ggctatccct cgatcctttt aatacctcga aatgggatca
     1621 aacagccatt gaagcgttga ttgatgcgat tgctccgatg cacgctgtct ggtataaaaa
     1681 agaaaaaacg attgctgaga tcaaaggctt caaaaatccg ttaaataccg accaaatgca
     1741 acgcttcata ccttattgga atgccctgga aaaagccgtg aaaacatcaa atttgccttt
     1801 tcttacaaag gaagattatt tattccatac tcatcttatt gaaactatgc cgcaatggca
     1861 tatcaaaatt gatgctatgg ctaaaacatt ggtacaaaat gactgtgtac cgaaaaatat
     1921 tgcattatgt aatcaaaacg ggacaaaaaa agttttggtt tatgattggg aaattgcaac
     1981 tattcatata ccacaacgcg atattgttga gtttttgtct tatgtcttac ctgaaaattt
     2041 cgatagtacg ttattaggaa attatataga gagacatcga ataaaacttg gcgaaacaac
     2101 aaaacaggcg attgatgcaa cagagtggcg actcggattt caatatgctt tatacgatta
     2161 tttaatacaa cgtgtttttc cacaaatacc ttttgaaaag cttgaagctc gtaacatagc
     2221 aaaagtgtac aacaatgccc gtcgcatgat ggcgttacta gactaatttt ctggagtgat
     2281 taaggatgac tcatatacgc gtaatactgc ctgggtacaa tactaaagtt ttaaacaagg
     2341 acatgttaaa accgtttgaa aaccctggcc tggaattaag tgttgattat gctgaccttg
     2401 cggttgatgc ggtacgacac gaatttgata ttgctttgat ggcgccttat ctcataaaaa
     2461 aagtaataaa agccgagcaa gaaaaggttc atgccatagt gattgatatg atggcagatg
     2521 tggcaatgcg cgcacttcgc gaggcagtga caattcctgt cgtcagtctt gctgaaacag
     2581 caacacatgt tgcagccatg atgggcaata attttgcgat tatcaataca acagatgaac
     2641 tgacgcccat tcaggaaagt ttggtccgta gttatgggct atatgatcgg ttggcatgta
     2701 tccaaggcat acaattggat ccgcatgacg attatcgctc agaaaaaata atccatggct
     2761 tagttgagca atgtgcggat gccgtgataa agaagcgtgc tgatacactt attttcgggt
     2821 cagggcgttt gattggctat cgtgaagtta ttcaaaaagg tttagaggaa aaagggttac
     2881 acatcaccat tattgagccc ctgcctaccg gaatttattt tgcaaaattt ctggtagact
     2941 ccaaaatttc ccaaagcaaa tatgtttatc ggtcacccga tgcttccacg ttaaaaacgt
     3001 attcaaaaat tttggggaat taatcctctt gattttatct cgaatctcat taggcctagt
     3061 ggttactgat aaactttctt tccatcaata taaacagcaa ctaacttgtt ttcgcaggat
     3121 ctggcaatca ctcttgccag acgatattga agttcatcaa gggctaactc ttgtgacaaa
     3181 gaagaaagct ctgccagagg atagttattc ctgtcatcca ggataatcaa atccgccttt
     3241 ttacctgctt caatgctgcc aatttgcttc tctaatccca gcaatttcgc tcctccaaga
     3301 gtagcatgat atagccaggt tttagcatca atcaaagctg tctttttatc aagcctccat
     3361 aatgctttat tagtaaaact cgctgaacgc atttcatcaa aaagagaaag actgtcccct
     3421 ccaccactgt ctgagcccaa tccaacctca atccccaagc ctttaaaaaa agaaaccggt
     3481 gccattccca tagcaaaaga aaaattactg gagggacaat ggctaattcc aacttgcttg
     3541 gcatagtctc ccatgcgttt catttcgtga agtgataaca aagtgccatg ggcaaacaca
     3601 gtcttatcag caatacattg tgtggattca aacacatcaa aataatcatg agctgtacta
     3661 aacaagcttt gaatcaattc atcaaaccct tcatccttat caagatgggt atgcaatatc
     3721 agcttgtttg cacgggcgaa ctcacctgct tgcctcaaca tagccgcaga acaagtcact
     3781 gcaaaccggg gagaaacagt gatatgtata ttatttttac cgtggtaacg atcttttaaa
     3841 acagtcagat ctttaaatgc ctgagcacaa tcaacaagca aattatcagg tgcgtttcta
     3901 tccatcaaaa ccaagcctat aaacgctctg ataccaaggc gggctgccac atcacaaact
     3961 atttctgtcg cttggcgact gctggttacc atcgtattga tagtcgttgt gccagaaccc
     4021 aggcaagcat gcatataaag ctcagcgtga cgttggcatc tttgcagatt actcgagaaa
     4081 gcggtttcag cgggaaaaac caaagtgttt aaccaatcca tcaatggcaa atcaccagct
     4141 ccacaaatag cgtattggga aagatggctg tgtgtatcaa ttaaaccagg aaggattaaa
     4201 tgatcaggat aatttgacac cgtataggat atttctttat tgaaatgagc ctgctcgccc
     4261 acctcaataa tgctatcacc attgatcaag acatagcctt tcttgataag aagactctgc
     4321 ccagcttgtg taggagtgat aatatcccct aaaatcagat gaggaatgct attcaattca
     4381 tatcctgaga tttatttctt actatcaaaa agtatagaca acctcctgga ttaactgaaa
     4441 ttcggattaa aaatacgata gtgaaaagaa ctccttcttt tcaatttaga tatgaaggag
     4501 ctcttttgta agataaataa cctgaattat tcatccaatg aactttattg gcctctcata
     4561 ggtaaaatca agctctctcg ttcagctgtt cttgcttttg aaatttcatc aatacgctga
     4621 tgagctaccg cttcatttcc tttgctagcc tgtgaaaaac gcagtgaaac gccacccggg
     4681 attaaactac gattaaagaa caatccacgg taatctgttg ctgagtaggc attaggccta
     4741 ttgacattga actccaaagt caagttttgc aatgctttaa caaccattga tttagcagca
     4801 ttaaccaagt gctcttgcat ttccgaagta aaattctcac tgtaccaatt caaaggtttc
     4861 ggcacatcaa tatcaataaa tttttcggga tgagattggg attttaattc tgcaatcata
     4921 tttttcaaga caaaatcatg cttggagaca attttttgct gacaaaaatt gcgttgaaac
     4981 aaccctggac ctgtaccatc atttgtttta cgctcaataa tagttagtcc tttttcccgt
     5041 aaagcaaggc catcaaattc ctgtctttca ccactttctg gagaaataac ctcaaaagat
     5101 ttgtaaagac tcactgccaa ccgctcggaa aaatccttga tagtcaaata caactgcaga
     5161 tcactggttt tcaggctgga aggttgaaca gattgcatca aaccctcagc aaggtgactt
     5221 gggccactca taacctgcgg attagaatca cctgcttttc cttccagttt tgcctttcct
     5281 ttctcacgat cattgattgg agactctagt aaactctctt catcatcaaa gattttcttg
     5341 cgcaaatcat ccaaggtttt ttcttcatca gaatcataat caatcaaatc taccccttcc
     5401 acggttgcac cctcttgagg aggataataa attaatggag acacagtttt gaaattttca
     5461 agattattca accagtcttg taatagttta tccactcctt cagattgact caactcttcc
     5521 atagcgcgta aatatcgctc ttgataatta cgcgattcag aggctgaagc tgtattctct
     5581 ttctcttgac tttttttgcc gtctatttcc ttacttctct ctttcacaat cactcttaat
     5641 gcttccagat actcaatcat aggaggaatt gccttttttc tttcgctgac aaaatgttgc
     5701 ctggctgcgt caattcgttt tttatagtcc tcttgtaatt cctgctttct gttggcccat
     5761 acacctgtta gctcctcccg gatagccttt gtttgggtct tgcctttcat catgatagag
     5821 ttcgcctctt cgacaaactg tttatagtag tttgcataag gagtattgcc tttcggatgt
     5881 gcgcttctca gaagatgcaa ttgttgtttc atttcatgga attcctgggc atagagtttt
     5941 ctttccagtt tcagactttt tagtttattt ttcagttgct taattacgaa ctcatcatca
     6001 ctgccaaatt cagaaattag ctgcggaatt cttttgattt cattatcaat attgttcaac
     6061 cttctctcat aaaaccctag aatttcgctg tgttttttca aatcaaaaac atcactagga
     6121 tttaaagttt gaaggtgctt aagacaatgc ctcataacat aagagtaggc tttaaataaa
     6181 tcaccattac tattggtttg cggatctgtc gaaaaatcaa tatcctcatc ttcccgtaaa
     6241 ctatcacgac gtctatcttc aaggaatttg agttctttta gtgctcgacg aagccctctt
     6301 aattgatacg ggatgttctt agtttcaggt aaaagtgtac gaaccccttc tgtagttcta
     6361 gccacttcat caatctcttt aatggtcttg tgcaaatcat cactcgattg agttttcttc
     6421 aatttatcaa tcaattaccc aattaattta tccaaaagac cataaacaac ttgctcttct
     6481 gccaattttt cattctttaa aagatcagca tacagtgttt tgaagtgggg tgttaccttg
     6541 ctatttctag caggggtatc cgatgcaatt agaacaggat tgtcaagatt aaactgacga
     6601 gttaaaagtt ctactcctcc ccttactata acgtcgttcc aatcatcatt aagatttgga
     6661 ggctctttga ctatcacttt ataaccagca aacactaatg ctttgcaagc cttttcaaat
     6721 tctaaattag ccagaggatt cgctacagga tcatcatgat ctttcagtaa aacaaccgtt
     6781 gacccgggag gataatgagt tttgacataa gccaaggtgg cgggtagttg agatacaccc
     6841 attgacgcca ataccgaata gttatttctg acctcatcca gactcgccac actggccgct
     6901 gtttcaactc cttcggcaat aaaaacaaca tcgacgttat ctgttttatt aataacagct
     6961 gctttgcctt ctcgcagagg attggtctgt cctaaatatc tcttgcaaaa aaaatgtgaa
     7021 ttttcagggt ttgttgcttt gttgccatcg gggtcaattt gtattatctg tagaccaacg
     7081 agttcatcat ccagattata aacaggggca accacataat ctttataagg tttgttttta
     7141 tcaaactctg gaataccaat agggcctttt aagcttcgta tctgtaattt ttcggccacg
     7201 tctatcggga tttttcgagt tcctacgata tacctttcag caggagtgcc ttttaaagga
     7261 acacttcttg cccataaatc attggctaat ttaatgcttt ttttatcgat gacatattta
     7321 ttcaactgat taatttctgg agttgttgtc ttgcccatac tgattacctc acttaaatga
     7381 agtgaaaatt ttggcatatt tagcttaagg aaatattaaa aaaacaacac aaaatgaata
     7441 taaattaatc aaataatttg caattgctgg taaaaaatag aagtaactta tctaatatca
     7501 aattataatt gtctctgatg taaatcatgg ataaaacata ggcagcgagc tatgaagatg
     7561 agaacagtat tcttcattct cttaataact caggacaaat catccaggca atacaccaat
     7621 caatcccgtc tgctttatcg aggcaaacaa gactgccagg tttaaatgaa accaaaatgc
     7681 gatgttcctc ttccacaaca agttggctga ccagtttatt cataaatggc aaatgcccta
     7741 caaacatcat gttttgtttt aattgattaa tctcttttgc caatggtatg acatcatcaa
     7801 gcggctcaag ccctgccctg gactcaacgc catttttgct gaaaatatga ccagacaaac
     7861 tctctgctgt ttgttgtgct cttaattttc cactgtggta tacacaggac acttcgattt
     7921 taagcaggga aaggaagtta cttaacaatt gaatatcttt ctgaccctga tcactcaaag
     7981 gtctttcagg atcaatatct ttatccaaac tgtcaccatg ttgaacaaga taaattttca
     8041 tctcaccctt ttaattcatt agctaattta ttaaagtgta gactcatttg aaataacaga
     8101 taagaaaagc ttgtaatgaa acctaaaatt tcagtattta ttgcaacaag tctcgacggt
     8161 tatattgcaa ggaatgatgg ctcaatcgat tggcttatag aagcaaattc tctcgcctct
     8221 cctggagagg attgtggata tcgatcattt attgccacca tagatacgat aattatgggt
     8281 cgatattcct atgaaaaagt tattacattt gatgaatggc catatgcttc cataccggta
     8341 atagtactga gctcaaaacc catacagata ccagaacatt tgagacatat catttcagtt
     8401 tctaaccaga gccctataga attagctagc gaattggctc attatggcat tcaacacgta
     8461 taccttgatg gcgggattac tatccagaga ttccttcaaa ataatctgat tgatgagata
     8521 accatcacta tgatacctgt tttgatcggt tctggccgcc ctttatttgg ttttcttgag
     8581 aatgatatta aactcaaaca cttgtcaacc actagctatc caggtggttt tgtccaaatt
     8641 aaataccata ttggtgaata gccagatact ttagtcctgt ttactctgta attgtaatgc
     8701 agcatgcaat tgctatgtta tgatgatata gtgccaattt atcagtaaca ggatacgaaa
     8761 tggacattcg ctatttgatc tatgcttggg gcagcagtat tttattcttt atttatcttt
     8821 atttaagtta tttggttatt attcaacgtc gtaaatatca tgtgctctat ttaaccaatg
     8881 atgataaagc atttattgcc cgctttcgcg cgcatgccaa ctttgctgaa tacgtaccat
     8941 ttactttaat tctgatggga atatctatta tacttaagat tcccccttta ttattaactg
     9001 ttttaatgtt ttctctgatt atagcacgct tgggacatgc ctatggtcta atcaaaaagg
     9061 aagcagaaaa acaatttaaa ccccgcattt attcggttgc aacaactttc attatcatgg
     9121 gtattttagc gctcttcaac atatttaaag caaccatact gtatatagct atataagtat
     9181 ttagattatt ttgcttgaga ttttttgacg atttgacctc tatgatttat gagcgaacct
     9241 atgaagatac tcataaaaag taatgaacaa gtataaattg gcttacactt atttcagaag
     9301 cactgcctta ctaaaacaac ccctattaag ttggagcatg acagattagt tcattaggga
     9361 atcgtattat taaatgaaat aacaggctca tcatactcct catcatcccc gctactgcaa
     9421 ttgtcatagc tccaatcttc ccatacatcg cctatagaaa gctcatcttt aaactcatca
     9481 tatcgattac ttaataaaga atacgtttgc tcaaaccatt catacatcgc agagggtaag
     9541 tcactataaa tatcaatttc aatacctttc atcagaccta tcttatcatt gagatcttcg
     9601 agttgattat gtaaatgctt aaccaccagt ttgtagtgct caaggttgta tttatcagga
     9661 tttaacaaga catggcttat cataaatttt gtttttaatc tcaatatatc tactttgaac
     9721 tgacaaattt ctttgttcaa accttgctta tcctttttga ataagaagaa ccaattaaaa
     9781 aggacatact ttaacctgaa ctccagttct ttcatttcta cttgcaacgc cgaattgaaa
     9841 tctttatctt tgagtagttc actataagag gactcgatat ggtttaaatc cttctcgaat
     9901 tctttcttaa
//
LOCUS       DACTJE010000121         9538 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.121, whole genome shotgun sequence.
ACCESSION   DACTJE010000121 DACTJE010000000
VERSION     DACTJE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.121"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(231..1178)
                     /gene="mdcH"
                     /locus_tag="I9760_002202"
     CDS             complement(231..1178)
                     /gene="mdcH"
                     /locus_tag="I9760_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit epsilon"
                     /protein_id="HAT5043964.1"
                     /translation="MKILFLFAGQGYHADSLFDIFRTDKKTMNLLKIMSSAAEIDFLQ
                     NDLEMTNPHSVQSIIGAYQLTLFSRLLPLFTNHQVDLAGYSLGEISAFLGSINANHQD
                     SFKLFRYRTQLMTSIVNNNIFAEYDLLSVVGQFILEEIQPVIAKHNCYIAIINSIQHV
                     VIGGKISDLRHLITALSQYPISHAKFLGVYLPSHTDFYSDKSKQFQRFLDENYGFYSL
                     KYPVISPLELNKIYNARQERALLGQELCTTLQWHKVCALIGEYQYNLIIDLGPGDSMT
                     TILNKANTELFNTPLITASHYNSLKGLCNAIISLTSSYQ"
     gene            complement(1175..2017)
                     /gene="mdcB"
                     /locus_tag="I9760_002203"
     CDS             complement(1175..2017)
                     /gene="mdcB"
                     /locus_tag="I9760_002203"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAT5043965.1"
                     /translation="MQIFQHSLPNPYKIARYFAKTAVQALYDEVALYPKPGLVSFVDS
                     GAHQDMNGALFFRSLFGLRHYFYQVGLHTALGYSPKNLVTFGLNAEKTMLKITGGINT
                     HRGAIFALGILCTSICKLSVKYHAFSIEDLHYAIIDQWAQYLEKEHQNTDTHGTLVTK
                     KYAVHDAKQTAIEGYKPVFDTYNELLNMTHDQTFFGLLTYQRLLLTIDDINILYRTGP
                     EGLEFARYHVQQGICQNNREKSIQKAIELHQLFSKKNISPGGVADMLGLIYFLRHVFS
                     REAK"
     gene            complement(1989..2612)
                     /gene="mdcG"
                     /locus_tag="I9760_002204"
     CDS             complement(1989..2612)
                     /gene="mdcG"
                     /locus_tag="I9760_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase
                     holo-[acyl-carrier-protein] synthase"
                     /protein_id="HAT5043966.1"
                     /translation="MNYLRHTLCYLDKNTTTAILPAPAQIDILRYWLKCDYPLIVTRQ
                     SENIPSGQIQLAIPYFIPARNQKLRTSYVVDDSWVISTRALPSLQEIFPSLELDTSSK
                     IQVYGSYCWQHLTREQYVQPSSDLDVLISYDNQSLFNLSALQKYLQKTLNIDCIDGEI
                     RFPALGDCSWAELLQIHSSDSILFKTTKKVTLVKRESLYANFPALLA"
     gene            complement(2609..3328)
                     /gene="mdcE"
                     /locus_tag="I9760_002205"
     CDS             complement(2609..3328)
                     /gene="mdcE"
                     /locus_tag="I9760_002205"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HAT5043967.1"
                     /translation="MMPNEKITLKQLIDHCFDTTKTTIKQQIVFGAGKIENKNFNIVG
                     TVEDTSFGVDESIEMARCILDIIKNNNTDPIVLLTDVAGQKLAVRDEWLGMYAYFAHL
                     LKCLHLARQNGNKIITLIYNQAIGGSFIAFGMMADRIYALKNAKLAVMWLEGMSKVTK
                     IDIDVLRKISETSPVFAPGVENFKKLGGLHQVLDLDEVSQQLLLTLQEPNISHDNRAE
                     LGKQYGGRKEAYDIIKAIENL"
     gene            complement(3329..4525)
                     /gene="mdcD"
                     /locus_tag="I9760_002206"
     CDS             complement(3329..4525)
                     /gene="mdcD"
                     /locus_tag="I9760_002206"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAT5043968.1"
                     /translation="MDKYQYTFSLPKKLQISPMIKVGVAGSGNLEVIIKPNSNFDKTD
                     IIVNTVISGFRNTWDAVIERFVEDYPYGGLSITLNDAGATPPVVSLRLRQAMETYQTG
                     YHKKDSYTEAAARNRIYSLVDENSFTEFLLDKDTPSPTLPQLNMQVETDDGVIIGIAK
                     MNGIDIAIASQQKDFIGGSVGEIHGAKINGLIKYAIKHQLPAIIFLIDSGGVRLQEAN
                     VGEIEISEIIRSILDARSAGIKTIGVICGNNGAFGGMGIISGTLDYLIVNQGARIGIS
                     GAEVIQAVKGVEVFDSSNRPLVWRVYGGRTRFLKDDVQGYTTNKTMDIRQEIKTALKT
                     LATTPSLSLNSILAEHEQLQKRITSAKNCREEGEWLKNNRPELYQQDIFSVSDQQFLE
                     LTNKGK"
     gene            complement(4534..6180)
                     /gene="mdcA"
                     /locus_tag="I9760_002207"
     CDS             complement(4534..6180)
                     /gene="mdcA"
                     /locus_tag="I9760_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAT5043969.1"
                     /translation="MDWNKNLKDYESRLIKIKPFFKKGKIVSEKNIVTLLETAIKPYD
                     KVCLEGDNQKQADFLARCLCSVNPQKIYGLHMLQSAVTLSEHLDLFERGIAEKIDFAF
                     AGPQSTRLAIMIQRNQVKIGNIHTYNELYARYVMDLAPNVALISVEKADYHGNLYTGA
                     NTEETPSIVEATAFKNGLVIVQANEIVRKLPRIDIPGDWVDAIVKAPSPSYIEPLFTR
                     DPALIDEVKILMAMMVIKGIYAPYQVNRLNHGVGFNTCAIELILPTYAQSLGLKGKIC
                     KHWMVNPLPTLIPAIEAGFIESIFCVGGEVGMNEFIRSKPDIFFTGRDGSLRSNRLYG
                     QLAGHYGCDVFIGATLQMDPQANSSTATKGRIAGFGGAPNMGCDTLGRRHSSYAWLKA
                     GQEKNSHNPVAMPRGRKLVIQMVETFQGAGKPTFVEKLDAWELQKEMQADLPPIMIYG
                     DDISHVVTEEGIANLLLCKNSEEREQAIRGIAGYTPVGLKRDKAIVDELRHRGIIQRP
                     EDLNISLKEADRDLLAAKNIHDLVEISNGLYSPPNKFRNW"
     gene            6463..7269
                     /gene="mavH"
                     /locus_tag="I9760_002208"
     CDS             6463..7269
                     /gene="mavH"
                     /locus_tag="I9760_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavH"
                     /protein_id="HAT5043970.1"
                     /translation="MDMREKYTGRSEPIINKPSDIPKGLVAKRKAELLLAKLEEAIKS
                     QESLNRESESHAKEDNSESLKPRLTHPTSERPKINHRPPSSFSYTNSESTLFISPKEE
                     KLEREEPSLSISSDKKKVVAKSSTSIWTELIAALSRAVDNYSHKRMEGTTPSEAYGWF
                     SWLRHGKAGEDKAGELASEARGTSTVDSLLTLLHHFFADSNTRYNNHSLAPYVMDELN
                     KLLIQYALKPFILSLGTQYDLNSWVHIKNQLSQLAGKEVSQERQSLVAPM"
     gene            7774..9120
                     /gene="mavG"
                     /locus_tag="I9760_002209"
     CDS             7774..9120
                     /gene="mavG"
                     /locus_tag="I9760_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavG"
                     /protein_id="HAT5043971.1"
                     /translation="MKEIIELLATLDELQKPYIHRVKTRLSELLNLDEKENELMRIEQ
                     STSRSWEEGATTDLRIGPIKSKKRIEEIRAVLITLSGAKAVKYEEGHNLENNAVEFIV
                     TGCDVEQLEGFTPPNQEEMQNTIPVMTFIRMSVAGNREYVRGISFNSEKLDGENYLGS
                     FKEGELNTSTQEWFSVLELLDLLNSESELNLIQIQNDLKSLLGVKFEVYFHSSSEEFP
                     IDLRTGSLDLNEAGAQQLLRAMDSLVLLGCLKTESKELEDGQKVCVIKQVNPQELHKF
                     SLKKEKNTNANSEMLSNIIELLKQSFPNNQVIRDLVVFDGREGRSMSRPYLHIRTQVY
                     HQEATSDYKSAMIILKKLQDKTSKKISFDFRNEMVEMKDESRPVYMSSIENTIKSSQF
                     IIDVAVGLTTQQLPELQDICAEINQSRLKHRHRHNLFKPQIEEQKINEQNQSLFLG"
     gene            complement(9209..>9538)
                     /locus_tag="I9760_002210"
     CDS             complement(9209..>9538)
                     /locus_tag="I9760_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043972.1"
                     /translation="EMYYLQYLSEKQNDPDLVNYQKNRNAIIYQWARAHNNDSLANAI
                     KNWEKLNKKQLSLTEHIAEEEKKKQWLKRIFTYNQIMASWVTLLAIHDEKALDDLEGL
                     LKCQNAS"
BASE COUNT         2874 a         1754 c         1887 g         3023 t
ORIGIN      
        1 ggcaccgtag tctccaatga cagcaaagac caggttttcc ttggccatca gcgggttagt
       61 caggataact aaaacaagaa acaacaagat tttcaaagaa gatccctgtg cttgatggag
      121 cgaagccgct atggtaagag atctcttttt aataaatcaa tagaattgaa agtacaagat
      181 gattaagtca atttaatttt taaaccgggc gaaatttaat gaatattatc tcattgataa
      241 gatgaggtaa gcgatattat ggcgttacaa agtcctttta aactattgta atgtgatgca
      301 gtaattaggg gtgtattgaa caattctgtg tttgctttgt tcaagatagt agtcatggaa
      361 tcgccagggc cgagatctat aatcaaatta tactgatatt cgccgattaa agcgcaaact
      421 ttatgccatt gaagtgttgt acatagctct tggccaagca aagccctttc ttgcctggca
      481 ttatatattt tatttaattc tagtgggctg atgacagggt attttagaga ataaaaacca
      541 taattttcat ccagaaatcg ctggaattgt ttgcttttat cagagtaaaa atccgtatga
      601 gaaggcagat aaactcctaa aaatttcgca tgacttatag ggtattggga aagcgctgtg
      661 atcaagtgcc ggagatctga aatttttccg ccgatcacca catgttgtat tgaattgata
      721 atagcaatat agcagttatg ttttgcaata acaggttgaa tttcttccaa tataaattgt
      781 ccaacgacag acaacaaatc gtattctgca aagatattat tattaactat cgatgtcata
      841 agctgggtgc gatacctgaa taatttaaag gaatcttgat gatttgcatt aatgctccct
      901 aaaaaagcgc tgatttcgcc taaactgtaa ccagccaggt caacttggtg gtttgtgaag
      961 agcggcagca gtcttgaaaa aagagtcaac tgataggcgc caataataga ttgaacagaa
     1021 tgcggattgg tcatttcaag atcattttgt aagaagtcaa tttcagctgc agaagacatt
     1081 atctttagaa ggttcattgt ctttttatca gttcgaaaaa tatcaaacaa actgtcagca
     1141 tgataacctt gaccggcaaa aagaaataat attttcattt ggcttctctt gagaaaacat
     1201 gccgtaaaaa ataaattaag cctaacatat ctgctacgcc cccgggactt atgttttttt
     1261 tggaaaataa ttgatgcagt tcaatggctt tttgtatgga tttttcccga ttgttttgac
     1321 atatgccttg ttgaacatga taacgggcaa attctaatcc ctcgggaccg gtacgataaa
     1381 ggatattgat gtcgtcaatg gtcaataata aacgttggta tgtcaataac ccaaagaatg
     1441 tctgatcgtg agtcatgttc aataattcat tataagtatc gaatactggt ttataccctt
     1501 caatggcagt ttgtttagca tcatgtacag catatttttt cgttactaat gtaccatggg
     1561 tatctgtgtt ttggtgttcc ttttccaggt attgagccca ttgatcaata atcgcataat
     1621 gcagatcttc aatagaaaag gcatgatatt ttacacttaa tttgcaaatc gaagtgcata
     1681 atatcccaag ggcaaatata gctcctcgat gagtattgat ccctccggta atctttagca
     1741 tggttttttc ggcattcaag ccaaatgtta ccagattttt aggtgaatat cccaaggcag
     1801 tatgcaggcc aacctggtaa aaataatggc gcaaaccaaa gagactgcga aaaaacaggg
     1861 ctccattcat atcttgatgt gccccactat caacaaaact gactaatccg ggttttggat
     1921 ataaggccac ttcatcatac agggcttgaa ctgctgtttt tgcaaaatat ctggcaattt
     1981 tgtatggatt aggcaaggag tgctggaaaa tttgcatata ggctctctct tttcactaaa
     2041 gttacttttt ttgttgtttt aaacaaaata gaatcactgg agtggatttg tagcaattct
     2101 gcccatgaac agtcgcctaa agcaggaaag cgtatttcac cgtctatgca atcaatgtta
     2161 agtgtttttt gtaaatattt ttgcaatgct gataagttaa agagtgattg attgtcataa
     2221 gatatcagca catccagatc agaagaaggc tggacatatt gctctctggt taggtgttgc
     2281 cagcagtagg agccataaac ctggattttg ctgcttgtat caagttctaa agaagggaag
     2341 atctcttgaa gagaagggag tgctcttgtg ctaattaccc aagagtcatc cactacatag
     2401 ctggtgcgta atttttggtt tcgtgctggg ataaagtagg gaatggcgag ttggatttgt
     2461 ccactgggga tattctcaga ctgcctggtt actataaggg gatagtcaca ttttaaccaa
     2521 taccggagta tatcaatttg tgcaggagcg ggtaagatcg cagttgtcgt atttttatca
     2581 agatagcata gagtatgtct gagatagttc atagattttc tatggctttt ataatgtcat
     2641 aggcttcttt gcggccgcca tattgtttgc ctaattcggc acgattatca tggctaatat
     2701 tgggttcctg aagtgtcaat agcaattgct gagagacttc atccaaatca agaacctggt
     2761 gtaaccctcc taattttttg aagttttcta cgcctggagc aaataccggg gatgtctcgc
     2821 tgatttttcg taacacatcg atatctatct tggtcacttt agacataccc tctagccaca
     2881 tgactgccaa tttagcattt ttcaaagcat atatcctatc tgccatcatg ccaaatgcga
     2941 taaaactgcc gccaatggct tgattgtata taagggtaat gattttattg ccattttggc
     3001 gtgccaaatg caaacacttt aataaatgag caaagtatgc atacattcct agccattcat
     3061 cacgtactgc taatttttgt ccggctacat cagtcaacag gacaatagga tctgtgttgt
     3121 tatttttgat tatatccaga atacatcggg ccatttctat ggattcgtca actccgaaac
     3181 tggtatcttc aacggtgcca acaatgttga aatttttgtt ttctattttt ccggctccaa
     3241 aaacaatttg ctgtttaatt gttgtttttg tcgtgtcaaa gcaatgatcg attaattgtt
     3301 ttaaagtaat tttttcattg ggcatcattt atttcccttt attagtcaat tccaggaatt
     3361 gctggtcaga aacactgaat atgtcttgct gatataattc gggtcggtta ttttttaacc
     3421 actcaccttc ctcacggcaa tttttggctg aggtaatgcg tttttgcagt tgttcatgct
     3481 ctgccagaat agaattcaga ctaagggacg gggtagtggc gagcgtttta agggcagttt
     3541 ttatctcctg acgaatatcc atggttttat tggtggtata tccttgaaca tcgtctttaa
     3601 gaaagcgggt tctaccacca tagactcgcc agactaatgg acgattactg ctatcaaata
     3661 cttcaactcc ttttactgcc tgaattactt ccgctcctga tatgccaata cgtgctcctt
     3721 gattaacgat aagataatca agagtgccac tgatgatccc catgccacca aaggccccat
     3781 tattgccaca aataacgcct atggttttaa tgccggctga gcgagcatcc aggatggagc
     3841 gaatgatttc agaaatttca atttcaccta cgttcgcctc ttgtaaacgg actccaccac
     3901 tgtcaatcaa aaaaatgatt gcaggcagtt gatgtttaat tgcatacttg attagaccat
     3961 tgattttggc accatgtatc tctcctactg agccgccaat gaaatctttt tgttgcgatg
     4021 caatggctat atcaattcca ttcattttgg cgataccaat gatgacccca tcgtctgttt
     4081 ccacttgcat gtttaactga ggcagggtgg ggctaggtgt gtctttatcg agtaagaatt
     4141 ctgtaaaact gttttcatcg actaacgaat aaattctatt gcgtgcggcg gcttctgtgt
     4201 agctatcctt tttatgatat cctgtttggt aggtttccat cgcctgacgt aggcgtaagg
     4261 aaacgacagg cggagtggcc cccgcatcgt taagagtaat ggatagcccg ccataaggat
     4321 agtcttcaac aaagcgttca atcactgcat cccaggtatt tcggaaacca gagattacag
     4381 tatttacaat aatatcggtt ttatcaaagt tagagtttgg tttaataatc acttccaaat
     4441 tacctgaacc ggccactcca actttaatca taggagatat ttggagtttt ttgggcaaac
     4501 tgaatgtgta ttgatattta tccataaata ggtctaccaa ttcctgaatt tgttaggagg
     4561 actataaagt ccattcgaaa tttccactag atcatggatg tttttggcag cgagtaaatc
     4621 gcgatcagct tcttttaatg aaatgttcaa atcttctggt cgttgaatga tgccacgatg
     4681 gcgcagttca tccactattg ctttatcacg tttcaaacct accggagtat aaccggcgat
     4741 tcctcggata gcttgttcac gctcctctga atttttacat aataacagat tggctatccc
     4801 ttcttcggta acgacatgag agatgtcatc accataaatc ataatgggtg ggaggtcggc
     4861 ctgcatttct ttttgtagtt cccatgcatc cagtttttct acaaaagttg gcttgcctgc
     4921 cccctgaaat gtttcaacca tttgtatcac aagtttacgt cctcgaggca ttgctacagg
     4981 attatgagag tttttttctt gtccggcttt aagccatgcg taagaggaat gacgtcttcc
     5041 taacgtatca cagcccatat tgggtgcccc gccaaatccg gcaattctac ctttggtagc
     5101 tgttgaactg ttggcttgcg gatccatttg aagggtcgcg ccaataaata catcacatcc
     5161 ataatgtccg gcaagttgcc cgtacagacg gttcgaacgt aatgaaccat ctcgaccagt
     5221 aaaaaagatg tctggtttgg agcgaataaa ttcattcatc cctacctcac ctcccacaca
     5281 aaagatagac tcgataaatc ccgcttcgat tgcaggtatt aaagtaggca gaggattgac
     5341 catccaatgt ttgcatattt taccttttag acccagagac tgggcataag ttggaagaat
     5401 gagttcaatt gcacaagtgt taaaaccaac cccatgattt aatcgattaa cttgataagg
     5461 agcgtaaatg cctttaatca ccatcatggc catgaggatt tttacttcgt caattagtgc
     5521 gggatctctt gtaaacaagg gttcaatgta ggatggagat ggggctttaa cgatggcatc
     5581 gacccagtct cctggaatgt caattcttgg gagtttcctg actatttcat tagcttgtac
     5641 aatgaccaag ccatttttaa aagcagttgc ttcaacaatg ctcggtgttt cctccgtatt
     5701 ggcacctgtg tacagattac catgataatc cgctttttct acagaaatta aggccacatt
     5761 gggggccaaa tccatcacat aacgtgcata cagctcattg taggtatgta tattgcctat
     5821 cttaacctga ttacgctgga tcataattgc caaacgtgtt gattgtgggc cagcaaacgc
     5881 aaaatcaatt ttttcagcga ttcctcgttc aaataagtca agatgttctg ataaggtaac
     5941 agccgattgc agcatatgta agccgtatat tttttgggga tttacagaac ataggcaacg
     6001 tgccagaaaa tcagcttgct tctgattgtc tccttccagg caaactttgt cataaggttt
     6061 tattgctgtt tcaagaaggg taactatatt tttctcagaa acgattttac cttttttaaa
     6121 aaaaggcttg attttaatga gtctgctctc ataatccttg agatttttat tccagtccat
     6181 aattttttag taatcagatg agaactatct aagtgatatg aaatgttaat agtatagtag
     6241 atattgacgc aattatttga ttttatcccc tgcttttttc ttttggcttg tgcaagataa
     6301 gttagcctat gatatttctc tgtaactgaa aaatactaaa tactttgttt cagtttattt
     6361 ctgttttctg gttgaacaga agagattaat ttcttgaaag aaaacaatat attgcattaa
     6421 cattagtgaa ctgtttaaat tcataaatca ttacataggg aagtggatat gagagaaaaa
     6481 tataccggtc gatcagagcc tattatcaat aaaccttctg atattcctaa gggattggtt
     6541 gcaaaaagaa aagcagaatt gctcttggca aaattagaag aagctataaa atcgcaagaa
     6601 tccttaaata gagaatcaga gagtcatgcg aaagaggaca attccgagtc actcaaaccc
     6661 agattgactc atccaacgag cgaacgtcca aaaatcaacc atagaccccc ttcttcattt
     6721 agttatacca actcagagag tactttgttt atttctccca aagaagaaaa gctcgagagg
     6781 gaagagcctt ctttatccat ttcaagcgat aaaaaaaaag tagttgctaa atcttctaca
     6841 tcaatttgga cggaactgat tgctgcctta tcaagagcgg ttgacaacta tagtcataaa
     6901 agaatggaag gaactactcc cagtgaagct tatggctggt ttagctggtt gcgtcatggc
     6961 aaggcaggag aagataaagc aggagagctt gctagtgaag cacggggaac cagtacggtt
     7021 gattctttgt tgaccttgct tcatcatttc ttcgcggact ccaataccag atataacaat
     7081 cattcattgg ccccttatgt aatggacgag ctcaataaat tattgattca gtatgctctt
     7141 aaaccgttca tactcagttt aggaacccaa tatgatctta atagctgggt gcatatcaaa
     7201 aatcaattaa gtcaactggc aggtaaggaa gtgagtcaag aaaggcaatc tcttgttgcg
     7261 cctatgtgat tatcaggagt cctgcacagg actccgtatt ttttaatcgc ttgagtttaa
     7321 cagtttaaat tttttttgaa ttgtttgatg attgcttggc tccaatcttt gcgatgcaga
     7381 aattgcagcg aatgccaaat taaatatggt tgtgtatgta ctgccaaaac accgccttaa
     7441 tatcctaata aataaattgt ggggatactc ctgcctgttt aagtaatcga tataggtata
     7501 tccaaaaatc tcttcgagcc ggtttccttc attttctgta aataatttga ttcatttaaa
     7561 acatcaccag ttattttaac aatttttagg ctccgttatg ggcgcatgga gtttcttatt
     7621 aagcaggata catattgttt ttaaattata aatgttgtgc tttttataca ttatatttta
     7681 tgttattgtt caatatcttg atcaataaaa tcaaaaagtt agatacaaaa actattttgg
     7741 aatagtcctt ttactttact tggaatttaa aagatgaaag aaattatcga gttattagct
     7801 accttggatg aattgcaaaa gccttatatt catcgtgtca aaactaggtt aagcgagctt
     7861 ttaaatctgg atgaaaaaga aaacgagcta atgcgaattg agcaatcgac aagccggtcc
     7921 tgggaagaag gtgctacaac cgatttaagg ataggcccta taaaatcaaa aaaaagaata
     7981 gaggaaataa gagctgtact catcacattg tctggcgcca aagcagtcaa atatgaggag
     8041 gggcacaact tagagaataa tgctgtggag tttattgtta cgggatgtga tgttgagcaa
     8101 ctggaagggt ttaccccacc gaatcaggaa gaaatgcaaa atactattcc tgttatgacg
     8161 tttattcgta tgtcagttgc agggaatagg gagtatgtgc gagggatttc attcaattct
     8221 gaaaaactcg atggagagaa ttaccttggt tcgtttaaag aaggcgaact aaacacgtcg
     8281 acacaagaat ggttttccgt tcttgaatta ttggatttat taaatagtga gtcagaactt
     8341 aatcttattc aaatacagaa cgacttaaaa tctcttttgg gggtgaagtt tgaagtttat
     8401 tttcattcat ccagtgaaga attccccatt gatttgcgta caggaagctt ggatctcaac
     8461 gaggcaggcg ctcaacaact actgagagcg atggatagcc tcgttttgct tggttgttta
     8521 aaaacagaaa gcaaagaact tgaggatggc cagaaagtat gcgttatcaa gcaggtaaat
     8581 cctcaggaac ttcataagtt ttcacttaaa aaagaaaaga atactaatgc aaactcagaa
     8641 atgctctcca atattattga acttttaaag caatcttttc caaataatca agtaatccgg
     8701 gatctggttg tctttgatgg cagagaggga aggagtatgt cacgacccta tcttcatatt
     8761 agaacccaag tctatcacca ggaagctact agtgactata aaagtgcgat gattattctc
     8821 aaaaaacttc aggataaaac atcaaaaaaa atcagctttg atttcagaaa tgagatggtt
     8881 gagatgaaag atgaatcaag acctgtatac atgagcagta ttgagaatac tattaaatca
     8941 tctcaattta ttattgatgt tgcggttggt ttaacaacgc aacagctgcc cgaattgcaa
     9001 gatatatgtg ctgaaataaa tcaatccaga ttaaaacatc gtcatcgcca caatctcttc
     9061 aaaccgcaaa ttgaggaaca aaaaattaat gagcaaaatc aatctttatt tttaggttaa
     9121 agtcaaattc gacataagag gtataagatg ccttttatgt cgccacttta gcattatgct
     9181 ttgttatctt atcctgcaat atttttattc aactagcatt ttggcatttt aacaaacctt
     9241 cgagatcatc tagggccttt tcgtcatgga tagctaatag cgtgacccag gaggccatga
     9301 tctgattgta ggtaaaaatc cgctttaacc actgtttttt cttttcttct tcagcaatgt
     9361 gctcagttaa ggaaagctgc tttttgttta gtttttccca attttttatt gcgttcgcca
     9421 aagaatcatt gttgtgggct cgtgcccatt ggtaaataat tgcgttgcga tttttttgat
     9481 agttcactaa atcaggatca ttctgttttt ctgatagata ttgcaaataa tacatttc
//
LOCUS       DACTJE010000122         9515 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.122, whole genome shotgun sequence.
ACCESSION   DACTJE010000122 DACTJE010000000
VERSION     DACTJE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.122"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1117)
                     /locus_tag="I9760_002211"
     CDS             complement(<1..1117)
                     /locus_tag="I9760_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1692 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043973.1"
                     /translation="MARSKEEKALEPKDAENKFRVLTSDVAGNIPTHSSVTDVLNLIY
                     SSITTHNLFKPHLEPLKAKACVVQGDLATLILIAQRNPAALFQKGNITDPRERTFYNV
                     SAWQLILFLCDVDMKNQLMPFIPEQFKAIAQKQTNELGSGGADLIKLSRDPQAVAFED
                     FDGLTQFKQTLPLIDGTRKEVTFPLLENVDGIVYFQDKNKEVHFYYANRESKTITSLV
                     PDLKSEEDKQLFAAFKASFDAMENNSSRRSSDTEHQLIAKLFNAKPQRQGIQYEHQGI
                     RYQDSRTSFSLINAYRTSIRLYDEVQNYQYEKADTYWREVVGKAQGEEMWLLERICEE
                     GRPFHPLPADFKEFKRGFTFYNWITNKVESAFAGGKLA"
     gene            complement(1411..2805)
                     /locus_tag="I9760_002212"
     CDS             complement(1411..2805)
                     /locus_tag="I9760_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT5043974.1"
                     /translation="MNHSKKVLNVFSLVMINVIAVDSLRTLPISAKLGFSLVFYYIFA
                     ALTFFIPVALVAAELATAYPNTGGIYVWVREAFGRRAGFITIWLQWIYNVVWYPTMLA
                     FIAATLSYLIAPHLGNNKFYLLGTALTLFWVFTFLNCFGMKLSSIVSIIGASIGTLLP
                     MIVIIVLGAVWIFQDRPVAVNYPTTWLPDFSSLGNLSLFSAVLFGLIGMEMSAVHAEE
                     VKNPQRDYPKALFYSALLIISTLSLGSLAIVIVVPNDSLSVVSGLVDAYAIFFNSYNM
                     PWMTSVIAVLIILGGLSGVSAWIIGPTKGLLVSARDGSLPALFSRVNKYGSPVAILLT
                     QGVIFTVLSTVFILLDSINAAYWVLSDLSAQMALLVYIMMFAAAIKLRYSKPEQPRGY
                     TIPGGNLVMSLISGIGIICCIAAMIVGFIPPSQIPIKNVFLFECFLIGGLILFVFIPW
                     LFAKKHDEQLCSEE"
     gene            complement(3070..5745)
                     /gene="acnA"
                     /locus_tag="I9760_002213"
     CDS             complement(3070..5745)
                     /gene="acnA"
                     /locus_tag="I9760_002213"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAT5043975.1"
                     /translation="MKVGQDSLSTKSQLTVDGKTYNYYSLKEAENKHFKGINRLPYSL
                     KVLLENLLRFEDGNTVTTKDIKAIADWLHNKTSQHEIAFRPTRVLMQDFTGVPAVVDL
                     AAMRTAIVKMGGNADKISPLSPVDLVIDHSVMVDKFASADALEVNTKIEIERNKERYE
                     FLRWGQKAFSNFQVVPPGTGICHQVNLEYLGKTVWNSENDGQLYAYPDTLVGTDSHTT
                     MINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLSGKLKEGITATDLVLTVTQM
                     LRKKGVVGKFVEFYGPGLNDLPLADRATISNMAPEYGATCGFFPVDKETIKYLELTGR
                     DKHTIALVEAYAKAQGMWYDKDNEEPVFTDSLHLDLGSVEPSLAGPKRPQDKVNLSSL
                     PVEFNNFLIEVGKEKEKEKTFAVKNKDFQMKHGHVVIAAITSCTNTSNPSVLMAAGLV
                     AKKAIEKGLQRKPWVKSSLAPGSKVVTDYLRHAGLQTYLDQLGFNLVGYGCTTCIGNS
                     GPLPDDISHCVAEHDLVVSSVLSGNRNFEGRVHPQVRANWLASPPLVVAYALCGTTCS
                     DLSREPIGQDKEGNDVYLKDIWPSNEEIAAEVAKVSGTMFRKEYAEVFKGDAHWQAIQ
                     TSSGQTYEWNPDSTYIQHPPFFENLSLKPEPLKPIKQAYVLALFGDSITTDHISPAGS
                     IKASSPAGLYLKSKGVDEKDFNSYGSRRGNHEVMMRGTFANIRIRNEMTPGQEGGVTR
                     YVPTGETMSIYDAAMRYQENQQDLVIIAGKEYGTGSSRDWAAKGTNLLGVKAVITESF
                     ERIHRSNLIGMGILPLQFKEGTTRKTLKLDGSERISIEISDKLTPGAMVPVTIERQDG
                     DIEKIETLCRIDTADELEYYKNGGILQYVLRKISS"
     gene            complement(5830..6456)
                     /locus_tag="I9760_002214"
     CDS             complement(5830..6456)
                     /locus_tag="I9760_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1689 family Dot/Icm T4SS effector"
                     /protein_id="HAT5043976.1"
                     /translation="MYHYLFSCHKSQESIDGLIEQVKQLLNHVEMEQKAYFLNLLTAR
                     VAEFQNELKSEASNTINKQQILIQYEKFAKTLLICIKQPERTSYAIHNYHKGFYYPVA
                     IHDKIKPDPTIENAAIATLGVSLALLLGSIPTFIFNPLFGVIMVSLAVTLLLPSGFYL
                     LIPDSPDTTSKKEEEKRIFMEGAKIINPDVRIEEFDEQPYLSSSLIKT"
     gene            6640..7884
                     /locus_tag="I9760_002215"
     CDS             6640..7884
                     /locus_tag="I9760_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HAT5043977.1"
                     /translation="MSDLKNLILNSSILLDTSSTVQNDLNPEQLINVHEDSDGGRDLL
                     TPESFILLMAEILSTNNADTICEYEHQNSADNKVLQNLQFKEVEEDEQIEGLNEQKKS
                     LDNPLWHDVDLPVINEISGNQCEGQFESTLEKNVALTWINSENFQPPIVKPENQTDET
                     YKIWDSEITSIESGKLKLDFPKADYDGNNINPYQKEAINQPGNDSSKNHQLIKGNDSF
                     PILEDIKTNAEPGTKAIKSEVEATKVGTEIPGSSFQMNQKAGSDIQPPVVSNSHIPKN
                     FSIPIHVNHSQWSEQLSEHIVWLGHQDVKSALIKIHPEELGPLEINVKVVKDNASVNI
                     STHSVYVKEIVDQAIPRLREMMAQQGINLSEVHIGTDTSSRQGSQKNHHAEIDLIQSA
                     EDKNKITSLTRHLPKGLVDYFA"
     gene            7921..9123
                     /gene="mavA"
                     /locus_tag="I9760_002216"
     CDS             7921..9123
                     /gene="mavA"
                     /locus_tag="I9760_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavA"
                     /protein_id="HAT5043978.1"
                     /translation="MSHNYFRTCLTTKDNKFFWQKPLASERFDKLIADRKFQKSIAHS
                     RKNQLMYSFIESKIASDYALLIDTKLREQLKQFTLDDFNDISGFERKEKSNSRQQSYF
                     KLRQELEFFLKNDIQQHKSCPDAQLNAFRRWINISDLLLRHHCYEGFLLVFVNLQLIA
                     DKQLIDGLPVSVRNNYNQLCQLSSPIGNHGALRHFMNTHQGESDFTPLFFTYHAIGAL
                     DESLESLKDKEVLLKKQLKHLNKKISHLRTEATPGVIDVISELLKNKQQIPKKMMERR
                     GHLIQLMEEVRFTGKQLKQIQVNMQNQLEQRANLVGLIVKEQKATPRTLPEYFEKTYS
                     IIKYRFNRQSIATVKSSQLPESTASSNPSSSNLYKSKLLPHFWNRHGKSSSSYWEEVF
                     TPSCLNNK"
     gene            complement(9174..9491)
                     /locus_tag="I9760_002217"
     CDS             complement(9174..9491)
                     /locus_tag="I9760_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5043979.1"
                     /translation="MKIVSAYVLRLFAILNLTFLLFITISFTNVASASNTNADQYLSG
                     NTQLAYFIGVHSYPGNYYWRGPVYYKNAYWTKWRYIGYGCQKSCLIDRWSGRVIRCAK
                     RCF"
BASE COUNT         3031 a         1795 c         1812 g         2877 t
ORIGIN      
        1 ctgcaagttt cccccccgcg aaagcagatt ctaccttgtt ggtaatccaa ttataaaagg
       61 taaatccccg tttaaactct ttaaaatctg cgggtaaagg gtggaatgga cggccttctt
      121 cacaaattcg ctctaaaagc cacatctctt caccttgtgc tttacctacc acttcgcgcc
      181 aataggtgtc ggctttttca tattgataat tttgtacttc gtcatacagt ctaatacttg
      241 ttctataggc attaatcaaa ctaaatgaag tgcgactatc ctggtaacgt attccttggt
      301 gctcatactg aatgccttga cgctgaggtt tggcattaaa tagctttgca atcaattgat
      361 gctcagtatc acttgagcga cgactggaat tgttttccat ggcatcaaag gaggccttaa
      421 atgcagcaaa taattgttta tcttcttctg acttgaggtc tggtacgaga gatgtaatcg
      481 ttttggactc tcgattcgca tagtagaaat gaacttcttt attcttgtct tggaaataaa
      541 caatcccatc aacattttca agcaatggga aagtgacttc ctttcgtgtc ccatcaatga
      601 gggggagagt ttgtttaaat tgagttaacc catcgaaatc ttcaaaagcc acggcttgtg
      661 ggtcacggga taatttaatt aaatcagcgc cgccactgcc aagctcgttg gtttgctttt
      721 gagctatggc cttaaactgc tctggaatga aaggcatcag ttgattcttc atgtccacat
      781 cacacagaaa caggataagt tgccaggcag atacattata aaaagttcgc tctcttgggt
      841 cggtgatatt tcccttttgg aataacgcgg cagggttgcg ttgtgctatc aatattaatg
      901 tagctaaatc gccttgcacg acacaagcct tggcttttaa tggttctaaa tggggtttga
      961 ataagttatg ggtggtaatg gaagaataga ttaaatttaa tacgtccgtt acagaggaat
     1021 gagtgggtat atttccagca acatcgcttg ttaagactct aaacttattt tctgcatctt
     1081 ttggttctaa tgccttctct tctttgctgc gtgccataca tacattcggt tattttcaca
     1141 atatgattga gcattataca ttaaatttaa tgttcatata aaaacaattt aatgtggcaa
     1201 atattatggc aattatcgaa aggtagaaaa caatactaag cagccttggt gatttaaaaa
     1261 agcatattat gcgtgagaat attgcgaaag aagaggctgt aaaactcgca gtttgaagtt
     1321 tattgggtat attacaattg attaacaact tttaagaatc atcttgaaaa gcccaacaaa
     1381 attttttgtg ggcttacatt tcttgctcaa ttattcttca ctacataatt gctcgtcgtg
     1441 ttttttagca aataaccagg gaatgaaaac aaatagaata aggccgccga tcagaaaaca
     1501 ttcaaagaga aaaacattct ttatagggat ttgtgaaggg gggataaatc cgacaatcat
     1561 agcagctatg cagcagatga tcccaattcc agagataaga gacattacca gatttccacc
     1621 aggaatagta tagccacgcg gctgttcggg cttgctgtat cgcaatttaa ttgctgcagc
     1681 gaacatcatg atatatacca acaatgccat ttgagcactt aaatcgctta atacccagta
     1741 cgcagcatta attgaatcga gtaaaataaa gactgtactt aatacagtaa atataacgcc
     1801 ttgagtgagc agtatggcta ccggcgagcc gtatttattg acacgggaaa acagtgcagg
     1861 taaggaacca tcgcgtgcag acactagtaa tcctttggtt ggacctatta tccaggctga
     1921 aacgccactg aggcctccta aaataattaa cacggcaatc actgatgtca tccacggcat
     1981 attataggaa ttaaaaaata tggcgtaggc atcaacaagg cctgacacaa cacttaaact
     2041 atcgtttggt actacaataa caattgccaa agaacctaat gataaggtgg atataattaa
     2101 aagagccgaa taaaaaagtg cttttggata atctctttgc gggtttttta cttcttcagc
     2161 atgaacagca gacatttcca ttcctataag gccaaataaa accgctgaaa ataaagacag
     2221 atttcctaaa gaactaaaat caggtaacca tgttgtgggg taattgactg ctacaggtct
     2281 atcttgaaat atccaaactg cgcccaggac aataatcact atcataggaa gtagtgtgcc
     2341 tatggaggcg ccgataatac ttacaatgct ggatagtttc atgccaaagc aattcaaaaa
     2401 agtaaacacc caaaacaagg taagtgctgt gcctaataaa taaaatttgt tattccccaa
     2461 atgaggagcg attaaatagg ataacgtggc ggcgataaaa gctaacatag ttggatacca
     2521 aactacgtta tagatccatt gcagccaaat agtgataaat cctgcccgtc tcccgaatgc
     2581 ttccctgacc cagacataaa ttccgccggt attaggatag gcggtagcga gctctgcagc
     2641 aaccaatgct actggaataa aaaaggtaag ggctgcaaaa atataataaa agaccagaga
     2701 aaatccaagt tttgcgctga taggcaaggt cctcaaacta tctacagcaa tgacgttgat
     2761 cataactaaa gaaaaaacgt tcagaacctt ttttgaatga ttcattattt atcctttatg
     2821 agtgcaataa acccgggtat tgaatggggt ataaatggta ttatgataag ttaacagagt
     2881 aaagaaaagt ttgtctgtac agtttaatta ctgcttaata tttataagtt gttgggtttc
     2941 tcctgattat ggtatcaatt tttactgata ttctgatttc agctgcctag tgatgcagtg
     3001 ttcatgcgtc tctagatatt ttgtgaaccc aacactctaa gggagaaggg cacgcaatgt
     3061 gcccaattgt taagagctta ttttgcgcaa tacatactga agtattccgc catttttata
     3121 gtattcgagc tcgtcagcag tatcaattct acaaagagtt tctatcttct caatgtcccc
     3181 atcttgcctt tctatggtta caggtaccat cgcgcccggg gttaatttat cagagatttc
     3241 aatactgatg cgttcgctac catccaattt taatgtttta cgagtagtac cctctttaaa
     3301 ttgcaatggc aatataccca taccaattaa attggaacga tggattcgct caaagctttc
     3361 agtaatcaca gctttaacac caagcaaatt agtgcctttt gctgcccaat cgcgagaaga
     3421 gcctgttcca tattctttac cagcaatgat aaccaagtct tgttgatttt cctgataacg
     3481 catggctgca tcataaatcg acatggtttc tccagtagga acataacgag tgacgccacc
     3541 ttcttgtcca ggagtcattt cattacgaat gcgtatattg gcaaaagtgc ctcgcatcat
     3601 gacctcgtgg ttaccgcggc gtgatccata ggagttaaaa tctttctcat ctaccccttt
     3661 ggattttaaa tataagcctg ctggagagct ggcttttatt gaacctgcag gtgaaatatg
     3721 atccgttgta attgaatcgc cgaatagggc taaaacataa gcctgcttga taggtttgag
     3781 cggttctggt ttcagactta aattttcaaa aaatgggggg tgttgtatgt atgtagaatc
     3841 aggattccat tcataagttt gccctgagct tgtttgaatg gcttgccaat gagcatctcc
     3901 tttaaatact tcagcatact ctttacggaa catggtaccg cttactttag cgacttccgc
     3961 agcaatctct tcattagatg gccagatatc cttgagataa acatcgtttc cttctttgtc
     4021 ttgcccaatg ggttccctgc ttaaatcgct gcaagttgta ccgcatagag cataagcgac
     4081 tactaatggg ggagaagcca gccagttggc tctgacttgc gggtgaacac gcccttcgaa
     4141 atttctatta ccagacagta cagatgaaac aacaagatca tgctctgcaa cacaatggga
     4201 aatatcatca ggcaaaggac ccgaattacc tatacaagtg gtacagccgt aaccaaccaa
     4261 attaaaaccc agttgatcca ggtaggtttg taggccggca tgccttaaat agtcagtaac
     4321 tactttagaa ccaggtgcca atgaagactt aacccagggt ttgcgctgta atcctttctc
     4381 aatcgctttt tttgctacca gtcctgctgc cattagtaca cttggattag aggtgttagt
     4441 acagctggtt atcgctgcaa tgactacatg accatgtttc atttgaaaat ctttgttttt
     4501 aacagcaaag gttttttctt tctctttttc tttaccaact tcaattaaaa aattgttgaa
     4561 ttctacaggt aatgaactga gattgacttt atcttggggg cgttttgggc cagccagaga
     4621 gggttcaacg ctgcctaaat ccagatgtaa agagtcagta aacacaggtt cttcattgtc
     4681 tttgtcatac cacatacctt gtgcttttgc ataagcctca acaagggcaa tggtatgctt
     4741 gtcacgacca gttaattcca agtactttat tgtttccttg tctaccggaa agaagccaca
     4801 tgttgcgcca tactcgggag ccatattgga aattgtcgct ctgtctgcca aaggtaaatc
     4861 attaagtcca ggaccataaa attcaacaaa tttaccaaca accccttttt ttcgcagcat
     4921 ttgagtgacg gtaaggacca aatcagttgc ggtaatgcct tctttaagtt tacctgataa
     4981 tttaaaaccg attacttcgg ggattagcat ggatacaggt tgccccagca tggctgcttc
     5041 tgcctctata ccaccgactc cccaacccag gacacctagt ccattaatca tggtcgtgtg
     5101 agaatctgtg ccgaccaaag tgtccggata ggcatacaac tggccatcat tttcactatt
     5161 ccacacagtt tttcctaaat actccaggtt gacctgatgg caaatccctg ttccgggtgg
     5221 gacgacctga aagttagaaa aagctttttg tccccagcgt aaaaactcat aacgttcttt
     5281 atttctttct atctcgattt tggtgttcac ttcaagggca tcagctgaag caaatttatc
     5341 taccatgact gaatggtcta tgaccaaatc gacaggagaa agaggagaaa ttttgtctgc
     5401 gttacctccc atttttacta tggcagtacg catggcagcc aaatctacta ctgcgggaac
     5461 accagtgaag tcttgcatca ggactctagt tggtctaaat gctatttcat gctgggatgt
     5521 tttattatgg agccagtcgg caattgcctt gatgtctttg gtcgttactg tattgccatc
     5581 ctcaaaacgt aataaattct cgagaagtac tttcaaggag taagggaggc gattaattcc
     5641 tttaaaatgt ttattttcag cttctttcaa actatagtaa ttataggttt ttccatcaac
     5701 tgttaattgt gattttgttg atagactgtc ttgacccact ttcataaagt actcccaaaa
     5761 tcattattat taataatctc taggaataat agctcaattt caggaagttt tcattcagta
     5821 tcaacaaaat catgttttga ttaatgaaga ggagaggtaa ggttgttcat caaattcttc
     5881 gattctaaca tcaggattaa tgatttttgc accttccata aaaatccttt tttcttcttc
     5941 ttttttgctt gttgtatctg gagaatctgg gattagtaaa taaaatccac ttggtaacag
     6001 cagtgtcacc gctagtgata ccatgataac accaaagagt gggttaaaaa tgaatgttgg
     6061 tattgaaccc aaaagcaaag caaggcttac tccaagagta gctatagctg cattttctat
     6121 agtgggatcg ggtttaattt tatcgtgaat agcaacggga taataaaatc ctttatggta
     6181 attgtgtatt gcataggatg ttcgttcagg ttgctttatg caaatgagca aggttttcgc
     6241 aaatttttca tactggataa gtatctgttg tttattaata gtatttgagg cctctgattt
     6301 aagttcgttt tgaaattcgg caacacgggc agtcaacaaa tttaaaaaat aagctttttg
     6361 ttccatttct acatgattta gtaattgttt tacttgttca atcaagccat ctatagattc
     6421 ctgagattta tggcagctga ataaatagtg atacatgata aatttctctc cctataaaat
     6481 atgtcaaaaa actagcatta tttttttagg ggttatagtg agtattaaga ggttttacat
     6541 gcatgcaata ttttttaact gtcccttgat tattttttca gtaagcttgc agatggtatg
     6601 agttttgcta tatgttaatc ataacacagg gagtgtgata tgtctgatct gaaaaacctt
     6661 atactcaatt catccatctt attggatacc tcttcaacag ttcaaaatga tctaaatcct
     6721 gagcaattga ttaatgttca tgaagattct gatggtggca gggatttatt aactcctgaa
     6781 tcttttatac tgctaatggc cgaaatatta tcaacaaata atgcagatac aatatgcgaa
     6841 tatgagcatc aaaattcagc agataataaa gttttgcaaa atttgcaatt taaagaggtg
     6901 gaagaggacg aacaaattga aggactcaat gagcaaaaaa aatccttaga taatccattg
     6961 tggcatgatg ttgatttacc tgttattaat gaaatatcag gtaatcaatg tgaagggcag
     7021 tttgaatcaa cactagaaaa aaatgtggct ttgacatgga ttaattctga aaatttccaa
     7081 ccaccaatag ttaaacctga aaatcagaca gatgagactt acaaaatttg ggactcggaa
     7141 ataacttcta ttgaatctgg caaactgaaa ctggattttc caaaagcaga ttacgatggg
     7201 aataacatta acccttatca aaaagaggca atcaatcaac cggggaatga ttcatcgaaa
     7261 aatcatcaat taattaaagg gaatgactct ttcccgatat tagaagacat taaaacgaat
     7321 gctgagccag gaactaaagc aataaaatct gaagtagagg caactaaagt gggcactgaa
     7381 atacctggta gttcctttca aatgaaccaa aaagccggtt cagatattca acccccagtt
     7441 gtttccaact ctcacatacc taaaaatttt tcaattccaa ttcatgtcaa tcattcacaa
     7501 tggtcagagc aattatctga acatattgtt tggcttggtc atcaagatgt aaaaagtgcc
     7561 ttaattaaaa ttcatcctga agaattgggg cctttggaaa ttaatgtaaa agttgtaaag
     7621 gataatgcct ctgttaacat atctactcac agtgtttatg taaaagaaat tgttgatcaa
     7681 gcgattccca gattaagaga gatgatggct cagcaaggta taaacttgtc tgaggtgcat
     7741 attggaaccg atacaagttc ccgccaaggt tcgcagaaaa accatcatgc tgaaatagat
     7801 cttatccaat cagcagaaga taaaaataaa atcacttcat taaccaggca tctacccaaa
     7861 ggactggtcg actactttgc ttagtttaac aaatcaattg ttggtaagga gtttacaaca
     7921 gtgtcccata attattttcg tacctgtctg acgacaaagg acaataagtt tttttggcaa
     7981 aaacctttgg catcagaacg gtttgacaag ctgattgctg atagaaaatt tcaaaaatca
     8041 atagctcaca gcaggaaaaa tcaattaatg tatagtttta tagagtctaa aattgcctca
     8101 gattacgcac tattgattga tactaaatta agagagcaat tgaaacagtt tactctggat
     8161 gattttaatg atatttcagg atttgaacga aaagaaaaat cgaattccag gcaacaaagc
     8221 tatttcaaat taaggcaaga gctggaattt tttcttaaaa atgacattca acagcataaa
     8281 tcttgccctg acgctcaatt aaatgcgttc agacgctgga taaatatatc ggatttactc
     8341 ttaagacatc attgttatga aggctttctt ttggtatttg ttaatttgca attaattgca
     8401 gacaaacagt taatcgatgg cttacctgta agcgtaagaa ataattataa tcaactatgt
     8461 caattaagtt caccaatagg gaatcatggc gctttgcgcc attttatgaa tacgcatcag
     8521 ggtgagagtg attttactcc tttatttttt acctatcatg ccattggagc tttggatgaa
     8581 tcgcttgaat ccttaaagga caaggaagtt cttttaaaaa agcaattaaa acatctgaat
     8641 aaaaaaatta gtcatttaag aacagaagcc actccaggag tgattgatgt catttctgaa
     8701 ttattgaaaa ataagcaaca aataccaaaa aaaatgatgg agcgtagagg acatctgata
     8761 cagctgatgg aagaggttag attcactgga aaacaattaa aacaaataca agttaacatg
     8821 caaaaccaac ttgaacaacg cgctaatttg gtaggattga tagtcaaaga acaaaaagca
     8881 acgccaagaa cgctccctga gtatttcgaa aaaacctata gtattatcaa gtacagattc
     8941 aataggcagt ctattgctac agtcaaaagc tctcagcttc cagaatcaac agcaagcagc
     9001 aatccaagtt cctcaaattt gtataaaagt aaattgctcc cccatttttg gaatagacat
     9061 ggaaaatctt cctccagtta ctgggaagag gtattcacgc caagttgttt gaacaataaa
     9121 taagagaatt agatgaactc tgcttcatac aagccctgtt tatatgaatt tggttaaaaa
     9181 caacgtttgg cacagcgaat gactcgacca ctccatcggt ctatcaggca gcttttctgg
     9241 caaccataac caatatatct ccattttgtc cagtaagcgt ttttgtaata tacggggccg
     9301 cgccagtagt agttaccagg gtagctgtga accccaatga agtaagcaag ttgagtattt
     9361 ccggaaagat attgatcagc attagtgttg ctggcacttg ctacattagt aaatgatata
     9421 gtaatgaaca atagaaaagt taagttcaga atagcgaata atctcaggac ataagcactt
     9481 actattttca tgataaattc cttttatcgc agata
//
LOCUS       DACTJE010000123         9371 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.123, whole genome shotgun sequence.
ACCESSION   DACTJE010000123 DACTJE010000000
VERSION     DACTJE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.123"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(63..1367)
                     /locus_tag="I9760_002218"
     CDS             complement(63..1367)
                     /locus_tag="I9760_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAT5043980.1"
                     /translation="MSLFNKEPDPPLAESLRPQHIDEVIGQSHLLGEGKPLRLCFMGS
                     KLHSMILWGPPGVGKTTIARLTAQAFDCEWIALSAVFSGVKDIRAAVEKAQEYLIHDK
                     QTILFIDEIHRFNKAQQDALLPYTESGLITFIGATTENPSFEVNSALLSRAQVYVLKP
                     LSEQELKLLFLRAHQRALSSLQFTEEAVDFLISCADGDARRLLNLLEQTKTACLTIKT
                     AVVDVELLHNVIVQNSRRFDKGGEAFYDQISALHKSVRGSNPDAALYWLCRMLDGGVD
                     PYYLARRIIRMSWEDIGLADPKAIQIANDAAATYERLGSPEGELALAQAVIYLSVAAK
                     SNAGYVAFNQAMEFIKRDKSREVPIHLRNAPTQLMKKLGYGHEYRYAHDEPHGYAAGE
                     CYLPEGMPEVNWYNPVQRGLETKIAEKLAFLRQLDKDVKKKI"
     gene            complement(1383..1991)
                     /gene="lolA"
                     /locus_tag="I9760_002219"
     CDS             complement(1383..1991)
                     /gene="lolA"
                     /locus_tag="I9760_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAT5043981.1"
                     /translation="MNKLFLILLLIFSHEVFSQTSAEVLQSKLNAIQTMTANFSQIVK
                     AKNREVSRSSGSMALQRPGRFRWQTKDPLEQLIVADGQKMWIYDVDLEQVTVKNQEKG
                     LGGTAALFLSGYDETLTHDFDVSEKQKGKLTVFDLKSKSAKENFQRIKLIFSQSTLIG
                     LELYDQLGQITDVKLVQIKSNPKLPAKLFQFKPPKGVDVVKQ"
     gene            complement(1994..4378)
                     /locus_tag="I9760_002220"
     CDS             complement(1994..4378)
                     /locus_tag="I9760_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAT5043982.1"
                     /translation="MAKQHSGKQAGIPKKSMPSFIIKRLCEGSFILILTGALFVLLSL
                     FTYSTNDPGWSHASRSGMSVSNSGGQVGAYIADALYFAFGYFAFLLPVAFVYIAWAIL
                     KDFRSFKALDRMVLLLRTVGFILMVSGGCGLLSINHRFEAVDTIHSSGGIIGQTVGNG
                     WYQMLNMEGATLLLLAMFLVGTTWLTGLSWIKAIELIGCYTLNLFTFLDKFIRKGMQI
                     ISENFNKEKLKTPLIKTEQLPKPDNEKKKSVPKLFQDKKDKEQEKATPVLIASEEKPE
                     IVKPTNEFKEIRPPKTITPGALPSLSLLDKGQPGKPMGGYTHEELESLSRDVEQHLLD
                     FGIQADVVAVHPGPVVTRFELQLAAGVKVSKLTALAKDLARSLSVISVRVVEVIPGKT
                     VVGLELPNHSRQVVRLSDVLSADVYQQAHSPLSLALGVDIGGHPMVVDLAKMPHLLVA
                     GTTGSGKSVGINAMILSILFKASPEQVRLIMVDPKMLELSVYDGIPHLLTPVVTDMKE
                     AASALRWCVEEMERRYRLMAALGVRNLAGYNTKITEAAANGQPLLNPLWKPVDSMDET
                     APELQALPYVVVVIDELADMMMVVGKKVEQLIARIAQKARAAGIHMILATQRPSVDVL
                     TGLIKSNIPTRISFQVSSKIDSRTILDQQGAEQLLGHGDMLYLAPGSGAPLRVHGAFV
                     DDKEVHRIADDWRSRGEPDYVDDILKMGNENGDGAFDDDSGGQSVEDDDPLYDQAVEF
                     VIQTRKASISAVQRRLKIGYNRAARMIEEMERTGIVGPLDGGYRDVLVTSVTED"
     gene            4451..5401
                     /gene="trxB"
                     /locus_tag="I9760_002221"
     CDS             4451..5401
                     /gene="trxB"
                     /locus_tag="I9760_002221"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAT5043983.1"
                     /translation="MSISNHHRLIILGSGPAGYTAAVYAARANLNPVLITGMQPGGQL
                     TTTTDVDNWPGDIEGLQGPALMERMQKHAERFDTQVIFDHIVKADLAQAPFTLQGDST
                     TYTCDALIIATGASARYLGLPSETAYQGRGVSACATCDGFFYRNKSVCVIGGGNTAVE
                     EALYLSNLASSVTLIHRRDSLRAEKILQDKLFEKAHSGNIKIIWNSTLDEVLGDGKKV
                     TGAMIRNLKTGETDQLAMDGIFIAIGHTPNTAIFQDQLAMRDGYILIKSGLDGMATST
                     SVPGVFACGDVADHVYRQAITSAGFGCMAALDAEKYLDSI"
     gene            5404..6072
                     /locus_tag="I9760_002222"
     CDS             5404..6072
                     /locus_tag="I9760_002222"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAT5043984.1"
                     /translation="MAYDSNYTFPDPETSDKQGLLAIGGVLTPKRVLQAYSQGIFPWY
                     EPGNPVLWWSPNPRLILIPNEFKISRSLKKTLKKPFKLTVDTAFQRVISYCATCSDRS
                     NKTWITSEMIETYTQLHEMGYAHSFEIWDGSELVGGLYGISLGHAFFGESMFHTITDA
                     SKVALHFLCRIMQSWNFDFIDCQLPTLHLMSLGAKIISRKEFLHMLQETLKYPDKKGN
                     WSID"
     gene            6127..6498
                     /locus_tag="I9760_002223"
     CDS             6127..6498
                     /locus_tag="I9760_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAT5043985.1"
                     /translation="MKQHSQGFLKLVTEAKKRINEISPQILKSKIDNNEKLIIIDVRE
                     TDEWETGKIPNAIHLSKGIIERDIEKIITDNQSNIVVYCSGGYRCALVADSLQKMGYT
                     NVLSLDTGLQGWLEAGYSLVK"
     gene            6629..6850
                     /gene="infA"
                     /locus_tag="I9760_002224"
     CDS             6629..6850
                     /gene="infA"
                     /locus_tag="I9760_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAT5043986.1"
                     /translation="MAKEDHIEMAGTVIDTLPNTMFRVELENGHIVTAHISGRMRKNY
                     IRILTGDKVKVELTPYDLSKGRIIFRDKG"
     gene            complement(7017..8375)
                     /gene="pmbA"
                     /locus_tag="I9760_002225"
     CDS             complement(7017..8375)
                     /gene="pmbA"
                     /locus_tag="I9760_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAT5043987.1"
                     /translation="MQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVAVNY
                     DRGFSVDVRMGEVETVAFSEDKGVGLTVYIGQRKGGASSTDTSPEALQSLVKAACEIA
                     KVSAEDPCFGLADKELMTKNHPDLDLYHSWDITPQHAIDLALKCESHALSLDKRITNS
                     DGVNVSSYESHHGYANTHGGEGFIHSTRHSVSCSVIAKEGDEMQRDYDYTTARNAENL
                     VSADLIAAKAVERALSRLGSKQIKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNS
                     FLLDSIGQQLFPEFVHIYEQPHLKGALGSAPFDGEGVPTRPNVLVEKGRLLQYVLGSY
                     SARKMGLRTTANSDGVHNLTIDPTCGDLGDLLKMMRKGLLVTELMGQGVNGITGDYSR
                     GASGYWVEDGVIQYPVDQITIAGNLKEMYRMILAVGSDINPNISMRCGSILIENMMVA
                     GK"
     gene            8421..9197
                     /locus_tag="I9760_002226"
     CDS             8421..9197
                     /locus_tag="I9760_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HAT5043988.1"
                     /translation="MSSQTNVYPANKLNLWLLLVLALAIVIPMTSYAGSDWQELTPGI
                     EYQDLEGGLLNPWSHIHVFRIDLNKNQMALVTAKNLAQKNASVDQFAEHSKALLSING
                     GFFDHEFNPLGLRINNNKQENPLKRISWWGIFYVKDNKPRITNIRNFHYDSNIDFAIQ
                     SGPRLLIRGNIPSLKAGVADRTALGITDDGKVIILVTTNAAMSTRQLAQIMRAPPLSC
                     SDAINLDGGSSSQLYAKIDSFQLNVHGFSNVSDAIIVKKI"
BASE COUNT         2848 a         2029 c         1701 g         2793 t
ORIGIN      
        1 ttggatttct tctttgggat aaattgggtg acaaacctaa cctggcttaa acagagaaca
       61 aatcaaattt tcttttttac atctttatct aattggcgca gaaaagcgag cttttctgca
      121 attttagttt ctaaacctct ttgtaccgga ttataccaat ttacttctgg cattccttca
      181 ggtaaataac attcaccagc cgcatagccg tgtggttcat catgagcata tcgatattca
      241 tgtccataac ctagtttttt cattaattga gtaggagcgt ttctcaagtg aatgggtact
      301 tctctggact tgtctctttt tataaattcc atagcttgat taaatgcgac atatcccgca
      361 ttgctcttgg cagcaacaga caaataaatt acagcttgcg ccaaagcaag ctcgccctcg
      421 ggtgagccaa gcctctcata agttgcagca gcatcattag cgatttgaat cgctttcgga
      481 tctgctaacc caatgtcttc ccatgacatt ctaataatac gtctggctaa ataatatgga
      541 tccacaccac catccagcat tcgacatagc caatataatg ccgcatcagg attagagcct
      601 cgtaccgatt tatgtaaagc agaaatctga tcgtaaaacg cttctccacc tttatcaaaa
      661 cgtcgactat tttgtactat cacattatgc aaaagttcta catcaacaac tgctgtttta
      721 attgttaggc aggctgtttt tgtttgctca agtaaattga gtaatctcct ggcatcccca
      781 tcagcacaag aaattaaaaa atcaacggct tcctcggtaa attgcagaga agataaagct
      841 ctttggtgag ccctaagaaa taagagtttt aactcctgct ccgataacgg ttttaaaaca
      901 taaacttgtg cccgtgataa taacgctgaa ttaacctcaa aagacggatt ttctgtagtt
      961 gccccaatga aagtaattaa gcctgattct gtataaggca ataaggcatc ttgctgagct
     1021 ttattgaatc tatgaatttc atcaataaat aagatagtct gcttatcatg aattaaatat
     1081 tcctgcgctt tctcaaccgc agctcgaata tccttaactc ctgaaaatac agctgataat
     1141 gctatccatt cacaatcaaa agcctgtgca gttaatcttg ctatagtagt cttaccgacc
     1201 ccaggtggcc cccataagat catagaatgc aatttggagc ccataaaaca aagtcttaga
     1261 ggtttaccct ctccaagcaa atgactttga cctatgacct catcgatatg ctgaggcctt
     1321 aaactttcag ccaatggtgg atcaggttct ttattgaata ggctcataaa tcaacattaa
     1381 aattattgtt ttaccacatc cacaccttta gggggcttga attggaacaa tttggcaggt
     1441 aacttaggat ttgacttaat ctgcactaat ttaacatcag taatttggcc tagctgatca
     1501 tataactcca gaccaatcaa agtactttga ctaaaaatta acttaattct ttgaaaattt
     1561 tcttttgcag atttggattt caaatcaaaa acggtcaatt tccctttttg tttttcactg
     1621 acatcgaaat catgagtaag agtttcatca tagccgctca aaaacaatgc agcagtacca
     1681 cctagtccct tttcctgatt tttgactgtg acttgttcaa ggtccacatc ataaatccac
     1741 attttttggc cgtcagcaac aattaattgt tccaatggat cttttgtttg ccagcgaaat
     1801 cgtccaggac gttgcaatgc catggaacct gaagatcgtg acacttcacg attctttgcc
     1861 tttacaattt gactaaaatt tgcagtcatg gtttgtatag catttaattt actctgcagt
     1921 acttcggcag aagtttgact aaacacttca tgcgaaaaaa tgagcaacaa aatgagaaac
     1981 aatttattca tattcagtcc tcagtcactg atgttactaa tacgtcacga taaccaccat
     2041 ccaatggacc gactattcct gtcctctcca tttcctctat cattctggca gcgcgattat
     2101 aaccgatttt taatcttctc tgtaccgcag aaatactagc cttgcgtgtc tgaattacaa
     2161 attctaccgc ctgatcataa agaggatcat cgtcttctac ggattgcccc ccactatcat
     2221 catcgaaagc accatcccca ttttcatttc ccattttaag gatgtcatca acataatcag
     2281 gttcacctct tgaccgccag tcatcagcga tacgatgaac ttccttatca tcgacaaaag
     2341 caccatgcac acgcaaagga gctccacttc ctggtgccag atataacata tcaccatggc
     2401 cgagcaattg ttcggctcct tgttggtcaa gtatggtgcg cgaatcaatt tttgaagaca
     2461 cctgaaatga tattcttgtt ggaatattgg atttaattaa acctgttaat acatccactg
     2521 aaggtctttg tgtcgcaaga atcatatgga ttcctgctgc tcttgctttt tgcgcaatac
     2581 gagcaatcaa ttgctccact ttcttgccaa caaccatcat catgtcagca agttcatcaa
     2641 ttacaacaac tacataaggc agtgcttgca attctggtgc agtttcatcc atagaatcga
     2701 cgggtttcca taatggattt aacaatggct gaccattagc tgccgcttcc gtaattttgg
     2761 tgttataccc tgccaaattc ctaacaccca acgcagccat taacctataa cgcctctcca
     2821 tttcctctac acaccagcgc agtgcgctcg ctgcctcctt catgtcggta acaacaggag
     2881 tcagcaaatg tggaatccca tcataaactg ataattccaa cattttggga tcgaccataa
     2941 ttaaacgcac ttgctctgga cttgctttaa aaagaatact taaaatcatg gcattaatac
     3001 caactgattt accagaacca gttgttcctg caaccagtag atgaggcatt tttgccaaat
     3061 caaccaccat agggtgcccg ccaatatcaa ctcccaaggc taaagataag ggagagtgag
     3121 cttgctgata tacgtcagca gataaaacat ctgataacct gaccacttgg cgtgaatgat
     3181 tgggtaattc aaggcccaca acagttttac caggaataac ctctacaact cggacagaaa
     3241 taactgataa cgaacgagcc aaatcctttg ccaaagcggt cagtttgctc actttgaccc
     3301 ctgctgccaa ttgcaattca aatcgggtta ccactggccc cgggtgaacg gctacaacat
     3361 ctgcctgaat accaaaatca agcaagtgtt gttctacatc tcttgaaaga ctttccaatt
     3421 cctcatgtgt ataacctccc atcggcttcc ctggttggcc tttatctaac aacgacagac
     3481 tgggaagagc tccaggggtt attgttttag gaggacgaat ttccttgaat tcattggtag
     3541 gtttgacaat ttcaggtttt tcttcacttg caattaaaac aggtgttgct ttttcttgct
     3601 ctttatcttt cttgtcctgg aataatttag gaacagattt ctttttttca ttatcaggtt
     3661 tagggagttg ttcggtcttg atcaatggtg tttttaattt ctctttgtta aaattttcag
     3721 aaataatttg cattcctttt ctgatgaatt tatcaagaaa tgtgaaaaga tttaatgtgt
     3781 aacatccgat taactcaatt gcctttatcc aggataatcc agtcagccag gttgttccca
     3841 caagaaacat tgctaataaa agtaaagtgg cgccttccat gttaagcatt tggtaccaac
     3901 catttccaac tgtttgtccg ataatacctc ctgaactgtg aatggtatct actgcttcaa
     3961 agcgatgatt tatactcaat aaaccacaac caccagaaac catgaggata aaaccaaccg
     4021 ttctcagtaa cagaaccatt ctgtccaacg ctttaaaaga gcgaaaatcc ttcaaaatgg
     4081 cccaggcaat gtaaacaaat gctactggta ataagaaagc aaaatatcca aatgcaaaat
     4141 aaagcgcatc ggcaatatac gcaccaactt gtccccctga gtttgaaacg gacatacctg
     4201 aacgggaggc atgagaccaa cctggatcat tggttgaata ggtaaataaa gacaagagaa
     4261 caaaaagagc tcctgtcaaa atcaatataa aactcccctc acacaagcgt tttattataa
     4321 agctcggcat ggatttttta gggattccag cctgttttcc cgaatgttgt tttgccatag
     4381 gtattttaat gtttttattt ttgtcataat atttgccatc ttaacacaaa aaataaggaa
     4441 ggcagagaca atgagcataa gcaatcacca tcgcttaatc atactcggtt caggtcctgc
     4501 aggttacact gctgcagtct atgccgcaag agccaatctg aatcccgttt tgatcaccgg
     4561 aatgcagcct ggcggacaat taacaaccac gactgatgta gacaactggc ccggagatat
     4621 tgaaggatta caaggccccg ccctaatgga gcgtatgcaa aaacacgcgg aacgctttga
     4681 tactcaagtc atattcgatc atattgtcaa agctgattta gcgcaagctc cctttacact
     4741 acaaggggat agtactactt acacctgcga tgccttgatt attgcaaccg gagcctctgc
     4801 tcgttatttg ggattaccct cagaaacggc ctaccagggg cgaggtgtct cagcgtgtgc
     4861 tacttgcgat ggattttttt atcgcaataa aagtgtttgc gttattggag gaggcaatac
     4921 tgccgttgaa gaagcacttt atttatcaaa tttggcatct tccgttactc tgattcacag
     4981 acgagacagc ttgcgtgcag aaaagatact gcaggataaa ctctttgaga aagcgcattc
     5041 cggtaacatt aaaataatat ggaactcaac ccttgatgaa gtactgggcg atgggaaaaa
     5101 agtcacagga gccatgattc gtaaccttaa aactggtgaa actgatcaac tggcaatgga
     5161 tggtattttt atagccattg gacacacacc aaatactgca atatttcaag atcagctggc
     5221 tatgagagac ggttatatct tgattaagtc aggacttgat ggaatggcaa caagcacttc
     5281 tgttcctgga gtatttgctt gtggcgacgt tgctgatcat gtttatcgtc aagccattac
     5341 atcagcagga tttgggtgca tggcagccct ggatgctgaa aagtacctgg attcaatata
     5401 aaattggctt acgatagcaa ctatactttt cctgacccgg aaaccagtga taaacagggt
     5461 ttattagcaa ttgggggtgt tttgaccccc aagcgagttt tgcaagccta ctcccaagga
     5521 attttccctt ggtatgagcc cggtaaccct gttctttggt ggtcacctaa tccgcgcctg
     5581 atcttaatac ctaacgaatt taaaatatcc agaagtttaa aaaaaactct gaaaaaacct
     5641 tttaaactga ctgtcgatac tgcttttcaa cgagtcattt catactgtgc aacttgttca
     5701 gacaggtcta ataagacctg gatcactagt gaaatgatag aaacatacac ccaattacat
     5761 gagatgggat atgcgcattc ctttgaaatc tgggatggtt ccgaacttgt aggcggactc
     5821 tatggaatca gccttggaca cgcttttttt ggtgaatcga tgttccatac aataacagat
     5881 gcctccaaag tcgccttgca ttttttatgt cgcattatgc aatcatggaa ttttgatttt
     5941 attgattgcc agttgccaac cttgcattta atgagccttg gcgcaaaaat aataagtcga
     6001 aaagaatttc ttcatatgtt acaagaaact ttaaaatacc ctgacaaaaa gggaaattgg
     6061 tctattgatt aaatttttta tcgataacct tttatcttta taaaactaaa tagagtgaga
     6121 ataagcatga agcaacattc acaaggtttt ttaaaactgg taacagaagc aaaaaaacgt
     6181 ataaatgaga taagtcctca aattttaaaa agtaaaatcg ataacaatga aaaattaatc
     6241 ataatagatg ttcgtgaaac agatgaatgg gaaacaggta aaatcccgaa cgcaattcat
     6301 ttaagtaaag ggattattga gcgtgacatt gaaaaaatta tcacagacaa tcaaagcaat
     6361 attgtagttt attgcagcgg aggttatcgc tgtgctttgg tagccgatag tctacaaaaa
     6421 atgggatata ccaatgtatt gtctctggat actggattgc aaggatggct agaggcggga
     6481 tacagcttgg taaaataatt gaatatctcc caataatgag ccttgagcta tatctttgaa
     6541 caatgtaatt gcaagactgg attgccattt tccgcaattt agtgcactat ttcactttta
     6601 aaaatttgat acatcatttg agagacatat ggcaaaagaa gatcacattg aaatggctgg
     6661 tactgttata gatacgctac ccaacaccat gtttcgcgtt gagttggaaa atggacatat
     6721 tgttacggct cacatttcag gtcggatgag gaaaaattat atcagaattc tgactgggga
     6781 taaggttaag gtagagctga ctccttacga tctttcaaaa ggtcgtatca tttttcgcga
     6841 taaaggctaa atgcgtttca tccaatttct atagctgttc tctgtttcat taaagaaaat
     6901 ctgccggaat atagaaaaag ctggctgcaa gcagccaggt atatcgctaa aaatgttcca
     6961 gagaactatc atcaagatta cagagcaagt ggttaacctc gatggtttgg ggcattttat
     7021 ttccctgcaa ccatcatatt ttctattaaa atagagccac agcgcatgga aatattcgga
     7081 ttaatgtcac tgcctactgc caatatcatc cggtacattt ctttaagatt tcctgcgata
     7141 gtaatctgat caaccggata ttgtatgacc ccatcctcaa cccagtatcc acttgcccca
     7201 cgtgaataat ctccggtaat accattcaca ccctgaccca ttagttctgt aaccagcaac
     7261 cctttccgca tcattttcaa aaggtcacca agatccccgc aagtaggatc aatggttaaa
     7321 ttatgaacgc catcactatt tgctgttgtc cttaaaccca tttttcgcgc cgaataactg
     7381 ccaagaacat attgaagcaa gcgccccttc tcaactaata cattgggtcg cgtaggcaca
     7441 ccttccccat caaaaggggc gctccccaaa gcacctttta aatgtggctg ttcgtaaata
     7501 tgaacaaact ccggaaacaa ttgttgtcca atggaatcaa gcaaaaagga attttttcta
     7561 tataaatttg aaccactaat agcgctcaca aagctaccca ataatccact ggaaactctt
     7621 gaagaaaaaa taaccggagt catttgtgtt ttaatttgtt ttgatcccag acgacttaat
     7681 gccctttcaa cagctttagc tgctatcaaa tcagctgaga ctaaattttc tgcatttctg
     7741 gcagtcgtat aatcgtagtc tctttgcatt tcatcacctt ctttggcgat taccgagcaa
     7801 cttacgctat gtcgagtact atgaataaac ccctctccac catgggtatt ggcataaccg
     7861 tgatgagatt cataggaaga aacattaaca ccatctgaat tagtaatccg tttatctaaa
     7921 gacaaggcat gtgattcgca ttttaaagcc aaatctatag cgtgttgtgg ggtaatgtcc
     7981 caggaatgat acaaatcaag atctggatga ttctttgtca tcaattcctt atcagctaat
     8041 ccaaagcaag gatcttcggc actaaccttc gctatctcac aggctgcttt aaccaaagat
     8101 tgtaatgctt caggagaggt atctgtactg cttgctcccc ctttgcgctg acctatgtat
     8161 acggttaaac caactccttt atcttcgcta aaagcgactg tctccacttc ccccattctc
     8221 acatcgactg aaaaacctcg atcataattt actgctactg ctgcatcagt ggcaccttct
     8281 tttttggcaa gttctaaaac atcttttatt actcgattta aatctgttgt tgacttatat
     8341 acttcactat tgttattttg cggtttaatt tgcatatgag attccgtggt gttgaatgat
     8401 gtacataagg atacaataac atgtcttcgc aaaccaatgt ttatcctgca aacaaattga
     8461 atttatggct cttgcttgtt ttagcactcg ccattgtcat ccctatgact tcctacgctg
     8521 gcagtgattg gcaagaactg actccaggta ttgaatatca ggatctcgaa ggaggcttgt
     8581 taaacccttg gtctcatatt catgtatttc gtatcgatct taataaaaat cagatggcac
     8641 tggtgacagc caaaaacctt gctcaaaaaa atgcatccgt tgatcagttt gccgaacaca
     8701 gcaaagcttt acttagcata aatggcgggt tttttgatca tgaatttaat cctctgggac
     8761 ttaggattaa caacaataaa caggaaaatc cactaaagcg cattagctgg tggggcattt
     8821 tttatgttaa ggataataag cctcgtatta ctaatatacg caattttcat tatgacagta
     8881 acattgattt tgctattcaa agcggcccca gattattaat cagaggaaac atcccttctc
     8941 ttaaagccgg ggttgcagac agaacagcgt tagggatcac ggacgatggc aaagtgatta
     9001 tattagttac aacaaatgct gccatgtcaa ccagacaatt agctcaaatt atgagagcac
     9061 ctccattatc ctgtagtgac gcaatcaatt tggatggtgg aagttccagc caattatatg
     9121 ccaagattga tagcttccaa ctcaatgttc atggtttttc taatgtcagt gatgcgatca
     9181 tagttaagaa aatttagtat cattcaaggc aagcccctta taatcggttg ggttcaaact
     9241 ccttatagaa ttaaattcac gcgctccaac cacctgcaga accccctttt caaattccac
     9301 cttcgttttt gcgagatccg taatcccgaa tctgctatat cattcgtatg cataaatcac
     9361 aatcaagcca t
//
LOCUS       DACTJE010000124         9328 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.124, whole genome shotgun sequence.
ACCESSION   DACTJE010000124 DACTJE010000000
VERSION     DACTJE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.124"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1874
                     /gene="rpoB"
                     /locus_tag="I9760_002227"
     CDS             <1..1874
                     /gene="rpoB"
                     /locus_tag="I9760_002227"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAT5043989.1"
                     /translation="RIVVRVNDDETTAGETGVDIYNLTKYFRSNQDTCINQRPIVSTG
                     DRIQRGDVLADGPCTDMGELALGQNLLVAFMPWNGYNFEDSILISERIVHDDRFTTIH
                     IEELTCIARDTKLGTEEITADIPNVGESALSNLDESGVVYIGAEVKAGDILVGKVTPK
                     GETQLTPEEKLLRAIFGEKASDVKDSSLRVPSGMNGTVIDVQVFTRDGLEKDARAKSI
                     EEEHLARVRKDLIDERRIREEDIYHRVSHLLLDKVATGGPGSLKPGSKITQDYLDKVE
                     REKWFDIRIEDDAISQQLEQLSKQLELLTKEMEKRFNDSRKKIIQGDDLAPGVLKIVK
                     VYLAVKRRIQPGDKMAGRHGNKGVISIVVPVEDMPHMEDGTAVDIVLNPLGVPSRMNI
                     GQVLETHLGLAAKGLGRKIAQMLDEKQTPEAIKAYLEKIYNHDGVQRVNLKCLNDDEL
                     MTLADNLRAGVPMATPVFDGATEQEIKSMLQLADLPADGKTVLIDGRTGNKFDNPVTV
                     GYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAY
                     TLQEMLTVKSDDVGGRTKIYKNIVDGDHRMDPGMPESFNVLLKEIRALGIDIELEHD"
     gene            1963..6168
                     /gene="rpoC"
                     /locus_tag="I9760_002228"
     CDS             1963..6168
                     /gene="rpoC"
                     /locus_tag="I9760_002228"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAT5043990.1"
                     /translation="MSDLLGILKQQGQSEEFDAIKIALASPELIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKTFGPVKDYECLCGKYKRLKHRGVICEKCGVELALAKVRRERM
                     GHIELASPVAHIWFLKSLPSRIGLLLDMTLRDIERVLYFEAFVVVDPGMTELERGQLL
                     NDEAYLDAMEQYGDEFDARMGAEAIRDLLRQIDLEDEIRNLREELPTTNSETKIKKIT
                     KRLKLLEAFYESGNKPEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLNAPDIIVRNEKRMLQESVDALLDNGRRGRAITGTNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPKKMALELFKPFIFSKLEFRGLAT
                     TIKAAKKMVEREESVVWDILDDVIREHPILLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCTAYNADFDGDQMAVHVPLTLEAQLEARSLMMSTNNILSPASGEPIIVPSQDVVL
                     GLYYLTREKVNALGEGKIYSSAQEAQNFYEAGHLDIHAKIKIRMPKEDGETGYHLVET
                     TVGRAILAEILPKGMPFDYINRTMTKKVISKVIDSCYRKFGLKETVIFADQLMYTGFK
                     YATRSGASIGIEDMEIPDDKASIIEHADNEVREIESQFRSGLVTNGERYNKVIDIWSR
                     TNELVAKSMMSKIATEEVTDAKGNKVRQESFNPIFMMADSGARGSAAQIRQLAGMRGL
                     MAAPDGSIIETPITANFREGLNVFQYFISTHGARKGLADTALKTANSGYLTRRLVDVA
                     QDVVITEDDCGTDTGILMQPLIEGGDIVEPLHERVLGRVVASDVYIPTQTEPVVKAGT
                     LLDEEWVEKLEKHGVDQVMVRSPITCQTRFGLCAKCYGRDLARGHLVNTGEAVGIIAA
                     QSIGEPGTQLTMRTFHIGGAASRATAANNIQIKTKGVIRLHNIKTVTHENKNLVAVSR
                     SGEVTIVDEFGRERERYKVPYGAVISAQDNSPVEAGQVIATWDPHTHPVISEVSGRLK
                     FVDLIDGITMNRQTDELTGLSNIVIIDAKQRSAAGRDLRPMVKLVTDEGDDIYLAGTN
                     VPAQYYLPVDAIVNFEDGSLVGIGDVIARIPQERSKTRDITGGLPRVADLFEARKPKD
                     SAVMAEVSGLVNFGKETKGKRRLIINVSEDQCHEELIPKWRHISVFEGEHVERGEIIA
                     EGALNPHDILRLLGVGALANYIVNEVQDVYRLQGVKINDKHIEVIVRQMLRKRVITFA
                     GDSKFLVGEQVEESAMLQENDKLLAEGKQIARGTPILLGITKASLATESFISAASFQE
                     TTRVLTEAAVSGKVDELRGLKENVMVGRLIPAGTGYTYHQSRKAKRARVAAGGDSSAT
                     HTVTASDVEHALSEALNADNHEH"
     gene            6282..6662
                     /gene="rpsL"
                     /locus_tag="I9760_002229"
     CDS             6282..6662
                     /gene="rpsL"
                     /locus_tag="I9760_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAT5043991.1"
                     /translation="MATINQLVRKPRVDVKKKSNVPALESCPQRRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTSGVNDRKQGRSKYGTKKPKDKK"
     gene            6683..7210
                     /gene="rpsG"
                     /locus_tag="I9760_002230"
     CDS             6683..7210
                     /gene="rpsG"
                     /locus_tag="I9760_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAT5043992.1"
                     /translation="MPRRREVPKREILPDPKHHSELLAKFINVLMVSGKKSIAEKITY
                     GALSVMEERVKKIKKNEEDGSETGSSGSAGAVLRYFEEALDNVRPSVEVRSRRVGGAT
                     YQVPVEVRHDRSIALGMRWIVQAARTRGEKGMMLRLAGELMDAYENKGSAVKKREDTH
                     KMAKANQAFAHFRWN"
     gene            7225..9309
                     /gene="fusA"
                     /locus_tag="I9760_002231"
     CDS             7225..9309
                     /gene="fusA"
                     /locus_tag="I9760_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAT5043993.1"
                     /translation="MATPLKLYRNIGIAAHVDAGKTTTTERVLYYTGMSHKIGEVHDG
                     AATMDWMVQEQERGITITSAATTCYWSGMDKQFESHRINIIDTPGHVDFMIEVERSLR
                     VLDGAVVVFDSVAGVEPQSETVWRQANKYGVPRIVFVNKMDRMGANFLRVVSQIKQRL
                     GSTPVVLQLPIGAEEEFKGVIDLIKMKAIHWDEENKGMTFKYVDIPADLKSTCEEYRA
                     HIIEAAAEYSEELMEKYLEGEEFTEAEIKKALRHLTITNKVVPVFCGSAFKNKGVQAV
                     LDGVIEYLPSPTDIPDIQGVDEHGDVIHRKTSYDEPFSALAFKIATDPFVGTLTYFRA
                     YSGILKSGDTVYNSVKGKKERIGRLLQMHANSREEIKEVRAGDIAAAVGLKTVTTGDT
                     LCDQDKVVILERMDFPDPVIAVAVEPKTKADQEKMGIALGKLAQEDPSFRVHTDEESG
                     QTIIQGMGELHLEIIVDRMKREFNVEANVGKPQVAYRETLKQAVEQEGKFVRQSGGRG
                     QYGHVWLKIEPQEPGKGYEFINAIVGGVIPKEYIPAVDKGIQEQMQNGVIAGYPVVDV
                     KVTLFDGSFHEVDSSEMAFKIAGSQCFKQGALKAKPVLLEPIMSVEVVTPEDYMGDVM
                     GDLNRRRGLVQGMEDSPAGKIVRAEVPLAEMFGYSTDLRSATQGRATYTMEFCKYAEA
                     PTNIAEAIIKKQ"
BASE COUNT         3013 a         1618 c         2218 g         2479 t
ORIGIN      
        1 ctcgtattgt tgttcgagtg aatgacgatg aaacaacagc aggggaaaca ggggtagata
       61 tttataactt aaccaagtat ttccgttcta accaagatac ttgtatcaat caaagaccga
      121 tagtatcaac aggtgatcga attcaacgag gtgatgtcct ggctgatggt ccatgtacag
      181 atatgggtga attggctctt ggacagaatc tactggtagc gtttatgcct tggaatggtt
      241 ataactttga ggattccatt cttatatctg aacgaatcgt tcatgacgac agattcacta
      301 ctattcacat cgaagagtta acatgtatag ctcgagatac caaattaggt acagaagaaa
      361 ttacagcaga tattcccaat gtaggcgagt ctgcactctc caatttggat gaatctggag
      421 ttgtttacat aggtgcagaa gttaaagcag gtgatattct tgtggggaaa gtaactccta
      481 aaggagaaac ccaactaact ccagaagaaa aactgttaag agctatcttt ggtgaaaaag
      541 cttcagatgt taaagattca tctttgagag ttccatcagg tatgaatgga acagtgatcg
      601 atgtacaagt ctttacccgt gatggacttg aaaaggatgc ccgtgctaaa agcattgaag
      661 aagagcatct agctcgagtt cgcaaggatt taatagacga acggcgtatt cgtgaagaag
      721 atatctatca tagagtttct catttgctgt tggataaggt ggcaacaggc ggtcctggaa
      781 gcctaaagcc tggatctaaa atcacccagg attacctaga caaagttgaa agagaaaagt
      841 ggtttgatat tcgtatagaa gatgacgcaa ttagtcagca acttgaacaa ttatccaagc
      901 aactggaact tttaacaaaa gaaatggaaa agcgctttaa tgacagtaga aagaaaatta
      961 tccagggaga cgatttagct cctggtgtat taaaaattgt taaagtgtat ttggctgtta
     1021 aaagaagaat tcagcctgga gataaaatgg caggtcgtca tggaaacaag ggtgtgatct
     1081 ccattgttgt tccagttgaa gatatgccac atatggaaga tggaacggct gtagatatcg
     1141 ttcttaatcc attgggcgta ccatcgcgaa tgaatattgg ccaggttctt gaaacgcatc
     1201 tgggactagc tgccaaagga ttaggaagaa agattgcaca gatgcttgat gaaaagcaaa
     1261 cacctgaagc aataaaagca taccttgaga aaatttataa tcatgacgga gtgcagcgtg
     1321 ttaatttgaa atgcctcaat gatgatgaat taatgacact ggcggataac ctgagagctg
     1381 gtgtgccgat ggcaaccccc gtctttgatg gtgctactga gcaagaaatc aaaagtatgt
     1441 tgcagttagc agatttgcca gcagatggta agaccgtgtt gatagatggc cgaacaggta
     1501 ataaatttga taatccggtt actgtaggct acatgtatat gttgaagtta aaccatttgg
     1561 ttgatgacaa gatgcatgct cgttcaactg gatcatacag tttggtcact caacagccac
     1621 ttggcggtaa agcacagttt ggtggtcagc gttttggtga gatggaagta tgggcattag
     1681 aagcatatgg tgcagcatac actttacaag aaatgttaac cgtaaaatca gatgacgtcg
     1741 gaggtcgaac aaagatctat aaaaatatag tcgatggaga tcatcgtatg gacccaggaa
     1801 tgcctgaatc cttcaatgta ttattgaagg aaattagagc actgggaatt gatatcgagt
     1861 tggagcatga ctaactcatc agcgatacat actgactaac cattttttgg aggcatagac
     1921 ttggctaccc taagcaacga cccaatgaaa aaagcaccag taatgagtga cttacttggt
     1981 attcttaagc aacaaggtca aagtgaagaa tttgatgcaa taaaaattgc actggcgtca
     2041 cctgaattaa ttcgatcatg gtcctacggc gaagttaaaa aacctgaaac aatcaactac
     2101 aggacattta agcctgaaag agatggtttg ttttgtgcta aaacttttgg tccagttaaa
     2161 gattatgagt gtttgtgtgg aaagtacaag cgactaaagc atcgtggtgt tatttgcgag
     2221 aagtgtgggg ttgaattggc tcttgccaaa gtgagacgag aaagaatggg gcatatcgaa
     2281 ctcgctagcc cagtcgctca catatggttc ttaaaatcct taccatcaag aattggtttg
     2341 ctcctcgata tgacactaag agatatcgaa agagtactct attttgaagc ttttgtcgtt
     2401 gttgatcctg gaatgacaga acttgaaaga gggcaactac ttaacgacga agcctatctt
     2461 gatgcgatgg aacagtatgg tgatgagttt gacgcgcgta tgggcgcaga agcaattcgt
     2521 gatttattgc gccaaattga tctggaagat gaaattagaa atttacgtga agagcttcca
     2581 acaactaact ctgagacgaa gattaaaaaa atcaccaaaa gactcaagtt attagaagct
     2641 ttttacgagt caggaaataa accagaatgg atgatcatgg atgtacttcc agtgcttcca
     2701 cccgatttga ggccattggt tccgctagat ggtggacgtt ttgctacttc agacttgaat
     2761 gatttataca ggcgcgtcat taataggaac aaccgactaa aacgcctgct ggatctaaat
     2821 gcgccagaca ttatcgttcg taacgaaaaa cgaatgctgc aagaatccgt agatgcttta
     2881 cttgataacg gcagaagagg tcgtgccatc actggtacaa ataagagacc tcttaaatca
     2941 ctggcagata tgattaaagg taaacagggt cgtttcaggc aaaacctctt aggtaagcgt
     3001 gtcgactact ctggacgttc agttattgta gttggaccaa ctttaaaatt gcaccaatgc
     3061 ggattgccta aaaaaatggc gttggagtta ttcaagccat ttatattttc caaattggaa
     3121 tttagaggtt tggcaactac tatcaaagcg gccaagaaaa tggtcgaaag agaagaatcg
     3181 gtggtttggg atatattaga tgatgttatt agagaacatc ctatcctttt aaaccgtgct
     3241 cctaccttgc atagattagg tattcaagca tttgagcctg ttttaattga aggtaaagcg
     3301 atccagctcc atcctttggt atgtacagca tacaacgctg acttcgatgg agaccaaatg
     3361 gctgtgcacg tgcccttgac actggaagcg cagctggaag ctcgttcatt gatgatgtct
     3421 acaaacaata tattatctcc agccagtggt gagccaatta ttgttcccag tcaggacgtc
     3481 gtccttgggc tatactattt aacacgagaa aaggttaatg cattaggcga aggaaaaata
     3541 tattcaagtg ctcaggaagc gcaaaatttc tatgaagcag gtcatttgga tatccatgca
     3601 aaaattaaaa ttcgtatgcc taaagaagat ggtgaaactg gatatcattt ggttgagaca
     3661 acagtcggac gcgctatttt agctgagatt ttgcctaaag gaatgccatt tgactatata
     3721 aaccgtacaa tgacaaaaaa ggttatctca aaagtaatag acagctgtta ccgtaaattc
     3781 ggcttgaaag aaacagtcat ttttgctgat cagttaatgt ataccgggtt taaatacgcc
     3841 acacgatctg gagcatccat aggtattgaa gacatggaaa taccagatga taaggccagt
     3901 attattgaac acgccgataa tgaagtaaga gaaatcgagt ctcaattcag atcaggtctg
     3961 gtaacgaatg gtgaacgata caacaaggta attgatattt ggtcaagaac taatgaatta
     4021 gttgccaaat caatgatgtc taaaattgca acagaagaag tcactgatgc aaaaggcaac
     4081 aaagtaagac aagaatcatt taacccaatt ttcatgatgg ctgattctgg agctagaggt
     4141 tctgctgctc agatacgaca gcttgccggg atgcgaggtt tgatggcagc tccagatggc
     4201 tcaatcattg aaacgcctat cactgcaaac ttccgcgaag gtttgaatgt attccagtac
     4261 tttatttcta cacacggagc tcgtaagggt ttggcagata cggcgttaaa gacagcaaac
     4321 tcagggtatc tgacacgacg cttggtagat gttgcgcagg atgttgttat taccgaagat
     4381 gattgtggta ctgatacagg tattctaatg caaccactga ttgaaggtgg tgatattgtt
     4441 gagcctttgc atgaacgcgt ccttgggcgg gtggttgctt cagatgtcta tattcctact
     4501 caaactgaac cagtagtcaa ggctggaact ttactggatg aagaatgggt tgaaaaatta
     4561 gagaagcatg gggtagacca ggttatggtc aggtcaccaa tcacttgtca aacaagattt
     4621 ggtctttgcg caaaatgcta tggtagagat cttgctcgtg ggcatcttgt caacacaggt
     4681 gaagcagttg gtattatcgc agcacaatca atcggtgagc caggaacaca gttaaccatg
     4741 cgtaccttcc acattggagg tgctgcatca agggcaacag ctgcgaataa tattcaaatt
     4801 aaaacgaaag gggttattcg gttacataat ataaagaccg taactcatga gaataaaaac
     4861 ctggtggcag tttctcgctc aggtgaggta actattgttg atgagttcgg acgagaaaga
     4921 gagcgatata aagttcctta tggtgcagta atttcagcac aggataattc tcctgttgaa
     4981 gctggtcagg ttattgcaac gtgggatccc catacgcatc ctgttatttc cgaagtgtca
     5041 gggcgtttaa agtttgttga tctcattgat ggcattacaa tgaatagaca aactgatgaa
     5101 ttaactggat taagcaatat tgtaataatt gatgccaagc aacggagtgc agcaggtcga
     5161 gatttgcgcc caatggtaaa attagttaca gatgaagggg atgacatata tcttgctgga
     5221 accaatgtcc cggcacaata ttatctacct gtcgatgcca ttgtgaactt tgaagatgga
     5281 agtctggtgg gaataggaga cgttattgcc cgtattccgc aggaacgttc taaaacccga
     5341 gatattaccg gaggtttgcc cagagtagcg gacttatttg aagccagaaa accaaaggat
     5401 tctgcagtaa tggcagaagt atcaggattg gttaattttg gtaaagaaac caaaggtaag
     5461 cgacgcttga ttatcaatgt atcggaagat caatgtcatg aagagcttat tcctaaatgg
     5521 cgtcatattt cagtgtttga aggcgagcac gtagagcgtg gtgaaatcat cgcagagggt
     5581 gcacttaacc ctcatgatat attgcgcttg ttaggtgttg gagcgctggc taattacatt
     5641 gtaaatgagg tccaggacgt ctatcgttta caaggtgtga aaattaatga taaacacatt
     5701 gaagtaattg tcagacaaat gctacgtaag agagttatta ctttcgctgg tgactcaaaa
     5761 ttcctcgtag gagagcaagt agaagagagt gctatgcttc aggaaaatga taaacttctg
     5821 gctgaaggaa agcagattgc gcgaggtact cctattttac taggtatcac aaaagcttca
     5881 ttagctacag aatcgtttat atcagccgct tcattccaag agacaaccag agtcttgact
     5941 gaagcagcag taagcggaaa agttgatgaa ttgcgtggtt taaaagaaaa cgtgatggta
     6001 ggtcgcctaa ttccagcagg aacaggttat acttatcatc aaagccgaaa ggcaaaaaga
     6061 gcaagggttg cagcaggcgg agacagttca gcaacacaca cagttactgc aagtgatgtt
     6121 gaacacgcgt tgagtgaagc attaaacgcg gataatcatg aacattaatc aggattcaaa
     6181 aatggcagat ttaagcttga caaatctgcc gtacctatat agaatccggc ctccttatgc
     6241 attcaaaata ttcacaagtg acagatcgga gttaagtaca aatggctact attaatcagt
     6301 tagtaagaaa gcctcgtgtg gacgtaaaga agaaaagcaa cgtgccagca cttgagtcat
     6361 gtccacaaag acgcggtgta tgtactcgtg tatacactac tacaccaaaa aaacctaact
     6421 cagctatgcg taaggttgca cgtgtgcgct tgaccaatgg atttgaagta acatcctata
     6481 ttggtggtga gggtcataat ttacaagagc actctgttgt tttaattaga ggtggtcgtg
     6541 ttaaggattt acctggtgtg cgctatcata cagttcgagg tagcttagat acttcaggag
     6601 tgaacgaccg aaaacaaggg cgttccaagt atggtacaaa aaagccaaaa gataaaaaat
     6661 aaccgattgt aggaaaattg agatgccaag aagacgagaa gtacccaaac gagaaatttt
     6721 acctgatcca aaacatcata gtgaactgct tgctaagttc atcaacgttt taatggttag
     6781 tggtaaaaaa tcaattgctg aaaaaattac ttatggtgcg ctgtctgtta tggaagagcg
     6841 tgtcaaaaaa attaagaaaa atgaggaaga tggaagtgaa actggcagca gcggaagtgc
     6901 cggtgccgta cttcgttatt ttgaagaggc tttagataac gttcgcccaa gtgtggaagt
     6961 acgttcacgc cgagttggtg gcgcaacata ccaagttcca gtagaagttc gacatgatag
     7021 aagcatagcg cttggaatgc ggtggatagt tcaagctgct cgcacacgtg gtgaaaaagg
     7081 tatgatgctg cgtttggcag gggaactgat ggacgcttat gaaaataaag ggtctgcagt
     7141 aaagaaacgt gaagatacac ataaaatggc aaaagctaac caagcctttg ctcattttag
     7201 atggaattaa gagagagata agccgtggca actccattaa aactatatag aaacataggc
     7261 attgcagccc acgttgatgc tggaaaaaca acaactacag aacgagtatt gtactatacc
     7321 ggaatgtctc ataagattgg tgaggtacat gatggtgctg caactatgga ctggatggtc
     7381 caggagcagg aacgtggtat taccattact tctgctgcaa caacttgtta ttggtctggt
     7441 atggacaaac aatttgaatc ccatcggatc aatataattg atacaccagg acacgtagac
     7501 tttatgattg aagtcgaacg ctctttacgt gtactagatg gagcagttgt tgtatttgat
     7561 tcagttgccg gcgttgaacc tcaatctgaa acagtatggc gtcaagccaa taaatatggt
     7621 gttcctcgta tcgtttttgt aaataaaatg gataggatgg gtgccaattt cctaagagtt
     7681 gttagccaga ttaagcaacg attaggctct actcctgttg tgttacaatt accaattggc
     7741 gcagaagaag agttcaaagg tgtgattgat ctcattaaaa tgaaggcaat tcactgggat
     7801 gaagaaaata aaggtatgac atttaaatat gttgatattc ctgctgattt aaagtcaacc
     7861 tgtgaagaat accgtgcaca catcattgaa gcagcagcag aatactctga agagttaatg
     7921 gaaaaatatt tggaaggcga agaattcaca gaggcggaaa ttaaaaaggc tcttcgtcat
     7981 ctgacaatca caaataaagt tgttcctgta ttctgtggat cagcctttaa gaataagggt
     8041 gtccaagcag tactcgatgg agtaatagaa tatttaccat cacccactga cattcctgat
     8101 attcagggag ttgatgagca tggtgatgta atccatagaa aaaccagtta tgatgaacca
     8161 ttctcagcgt tagcgttcaa aattgcaact gacccatttg tgggtacgct aacatatttt
     8221 agagcttatt ctggtattct taagagtggg gatactgtct acaactcagt taagggtaaa
     8281 aaagaacgta ttggccgttt gttgcagatg cacgccaatt cacgtgaaga aattaaagaa
     8341 gtgcgagcag gggacatagc tgctgctgtt ggacttaaaa cagtaactac tggtgacacc
     8401 ctctgtgatc aagataaagt tgtaatatta gagagaatgg atttcccaga tccagttatt
     8461 gcagttgctg ttgaaccaaa aactaaagcg gatcaggaaa aaatgggaat tgctttaggc
     8521 aagttagccc aggaagaccc ttctttcaga gtacatactg atgaagagtc aggccagaca
     8581 atcattcaag gtatgggtga attacacctt gaaattattg ttgaccgcat gaagagagag
     8641 ttcaatgtag aagctaatgt tggtaaacct caggttgcat accgagagac actcaagcaa
     8701 gctgttgaac aagagggtaa gttcgtaaga caatcaggtg gacgtggtca atatggacat
     8761 gtatggttga aaattgaacc acaagaacca ggaaaaggtt atgaatttat caatgctatc
     8821 gttggtggcg tcattccaaa agaatatatc cctgcagtag ataaagggat tcaagagcaa
     8881 atgcaaaatg gtgtaattgc tggatatcca gtggttgatg ttaaagttac actgtttgat
     8941 ggttcattcc atgaagttga ttcaagcgaa atggcattta aaattgctgg gtctcaatgc
     9001 ttcaagcaag gtgcactaaa ggcgaaaccg gtgcttcttg agccaattat gagtgtcgaa
     9061 gttgttacac ccgaagatta catgggcgat gttatgggtg atctgaacag acgccgtggt
     9121 ttagttcaag gtatggaaga ctcgccagca ggcaaaatag ttagagcaga ggtgccactt
     9181 gcagagatgt ttggttattc aactgactta cgttctgcta cacaaggaag agcaacctac
     9241 actatggagt tttgtaagta cgcagaagca ccaacaaata ttgctgaagc aattatcaag
     9301 aaacaataaa agttaacgag gttattga
//
LOCUS       DACTJE010000125         9321 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.125, whole genome shotgun sequence.
ACCESSION   DACTJE010000125 DACTJE010000000
VERSION     DACTJE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.125"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            189..788
                     /gene="grpE"
                     /locus_tag="I9760_002232"
     CDS             189..788
                     /gene="grpE"
                     /locus_tag="I9760_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAT5043994.1"
                     /translation="MSKQNKKDWKKFKDEHKEEHKVENEILEEETDEESQHQEPALGH
                     PSYTALEEQLTLAEQKAHENWEKSVRALAELENVRRRMEREVANAHKYGVEKLISALL
                     PVVDSLEQALQLADKNSDPSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHEA
                     MSMQPAPGAPPNSVITVFQKGYKLSDRVIRPARVIVSTK"
     gene            933..2867
                     /gene="dnaK"
                     /locus_tag="I9760_002233"
     CDS             933..2867
                     /gene="dnaK"
                     /locus_tag="I9760_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAT5043995.1"
                     /translation="MAKIIGIDLGTTNSCVAVMEGDKPKVIENSEGHRTTPSIVAFTD
                     DNEILVGQSAKRQSVTNPEKTLFAIKRLIGRRFDDPIVQKDIKMVPYKIMKADNGDAW
                     VRVKDQDKAPPQISAEVLRKMKKTAEDYLGEEVKEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGMDKKRGDSVIAVYDLGGGTFDISIIEIAEVDGEHQFEV
                     LATNGDTFLGGEDFDLALIEYLASEFKKDTGIDLHNDPLALQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADASGPKHLNIKLTRAKLESLVEKLVERTIEPCKTALKDAGLTVSQI
                     NEVILVGGQTRMPLVQKTVEEFFGKEPRKDVNPDEAVAVGAAIQAAVLSGEVKDILLL
                     DVTPLSLGIETMGGVMTKLIEKNTTIPTKATQVFSTADDNQTAVTVHVLQGEREQASA
                     NKSLGRFDLRDIPPAPRGVPQIEVTFDIDANGILNVSAKDKATGKAQSIVIKASSGLS
                     EEEVAAMVKDAQSHAEEDKKFKEMAELRNQADSLIHSCEKSMKDLADELSEDEKRGIE
                     TAISELKEAVQGTDKARIEDKLKVLTDASAKMAERIYAKKSSEGQAAQGQTQSQESTK
                     PAEEGVVDAEFEEVKEEDKK"
     gene            3057..4196
                     /gene="dnaJ"
                     /locus_tag="I9760_002234"
     CDS             3057..4196
                     /gene="dnaJ"
                     /locus_tag="I9760_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAT5043996.1"
                     /translation="MEQRDYYELLEVSRNASDAEIKKAYRRLAMKYHPDRNPGDTSAE
                     EKFKEIQKAYNILSDKQKRAAYDQFGHAGVDPSMGGGPGGFGGFGGFGDVFEDIFENI
                     FSGGRGHGRQSRGQRGADLQFNVQLTLEEAAIGKEVEITVPRHGTCTVCEGSGAKKGT
                     SPKTCETCQGMGQVRIQQGFFSIQQTCPTCHGEGKIISDPCASCHGQGRVRESKKINV
                     KIPAGVDNGDRVRLSGEGEAGVHGGGSGDLYVQISLKKHAIFERHENDLHCEVPISFA
                     TAALGGSIEVPTLEGRVTLKIPAETQTGKVFRLRSKGMKSVRGYGQGDLLCKVVVETP
                     VNLSREQKELLNKLQDSLENAKGTHSPKTSSWFAGVKKFFEDMKF"
     gene            4292..5404
                     /gene="carA"
                     /locus_tag="I9760_002235"
     CDS             4292..5404
                     /gene="carA"
                     /locus_tag="I9760_002235"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAT5043997.1"
                     /translation="MINKSAILALADGTVYEGLSVGASGDSVGELVFNTSMTGYQEML
                     TDPSYARQIITLTTAHVGNTGCNMEDMESTKVWAAGLVMRNCTAIPSNYRAEQSFPDW
                     LSKNGVVAISGIDTRALTLKLREKGSIGACISTNVEKPEIVVAKAKSFAGLQGADLAL
                     EVTRQTIERWHEGRGMWSLDKQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTS
                     AEEVLAMNPNGVFLSNGPGDPQACDYAIKATQVFLDNNIPLFGICLGFQILALACGGI
                     TKKMKFGHHGSNHPVVETTGKKRVFITSQNHGFAVDEDSLPNCLEVTHRSLFDNSLQG
                     IRHKTKPAFGFQGHPEASPGPHDIDILFNEFLNLMK"
     gene            5613..6761
                     /locus_tag="I9760_002236"
     CDS             5613..6761
                     /locus_tag="I9760_002236"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAT5043998.1"
                     /translation="MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACE
                     VLVKTGMVLVGGEITTKAWVDVEEITRHVIKDIGYNSSQMGFDWESCAVLSAIGKQSP
                     DIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
                     WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWL
                     NDKTRYFINPTGRFVIGGPLGDCGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSA
                     AYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTGRLKNSEIIDLIHTH
                     FDLTPQGIIDQHDLLRPIYRQTATYGHYGRESFPWERLDKVAELSKAL"
     gene            6780..8105
                     /locus_tag="I9760_002237"
     CDS             6780..8105
                     /locus_tag="I9760_002237"
                     /EC_number="3.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HAT5043999.1"
                     /translation="MELVEEGVQKINTLQDYKVAEISLADWGRKEIAIAETEMPGLMA
                     LREEFSAKKPLQGARIAGCLHMTIQTAVLIETLVALGAEVRWSSCNIFSTQDHAASAI
                     AAKGIPVFAWKGETEEEYWWCVEQTLSGPNGWTPNLLLDDGGDLTQVVHQKHPKLLTA
                     IKGVSEETTTGVARLYEMAKHGQLKIPAINVNNAVTKSKFDNLYGCRESLLDGLKRAT
                     DVMIAGKVALILGYGDVGKGCAQALRGQGATVLVAEIDPICALQAAMEGYRVVTLDDV
                     AEQVDIVVTATGNYHVVTHDHMKRMRNQAILCNIGHFDSEIDIQSLKQYQWENIKPQV
                     DHVIFPDGKRIIILAEGRLVNLGCATGHPSFVMSASFTNQVLAQIELFQNSSQYQNQV
                     YVLPKVLDEKVARLHLGRIGAKLTQLSNEQADYIGVDKNGPYKPDHYRY"
     gene            8229..9236
                     /locus_tag="I9760_002238"
     CDS             8229..9236
                     /locus_tag="I9760_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT5044000.1"
                     /translation="MDLVIAANYLSTLEKCLEQFGINHFLEKIGFDLIRVHDPDAYLT
                     QDEFNQVILEAYKLSNCPELGLVFGQNLSIVNHGFLGYAAMSSPTFGTAIQTVLSYLN
                     TRTNLLSLELRQFNTENSAYIKLLPHTKEMVLSRFITELAITHLIKMRNFLISCNDPL
                     LKIELNYDEPPYAKYYQTIFQTKIIFNTEATRVWFKAEELNYPIHFADDVSYQLAKRQ
                     LQELEKKMSLKEDMASKIKSILINQNLNHVSMDEIASRLCMTSRTLRRHLQRLDVTYQ
                     ELLDEVREQKAKTFLLNNNISMTEICFLLGFQDTSNFSKAFKRWTGITPTEYREKHEV
                     Q"
BASE COUNT         2938 a         1584 c         2123 g         2676 t
ORIGIN      
        1 gaattaagac agatttttta gctatctaag tatggaataa atggacaatt actgcaagcc
       61 atgatttgaa aagtttcaat cagttaatga cctgaatttt aaaccaagat ttaaaatcct
      121 aacaggggct tgaaaattgc aaatacaatc cccatttgta ggaatcgagt tagtcaggag
      181 cgattttgat gagtaaacaa aataaaaaag attggaaaaa atttaaagac gaacacaaag
      241 aagaacacaa ggtagagaat gagattctgg aagaagaaac tgatgaagaa tcacaacatc
      301 aagaacctgc tttaggacat cccagctata ctgccttgga agagcaactg acattggcgg
      361 aacaaaaagc acatgaaaat tgggaaaagt ctgtacgcgc ccttgctgaa ttggagaacg
      421 ttcgtcgtcg tatggaacga gaagttgcca atgctcataa atatggcgta gaaaaactga
      481 tttcagcttt attgccagta gtggacagtc ttgagcaagc attacaatta gcggacaaga
      541 acagtgatcc atctatgcat gaaggacttg agttaactat gaaactcttt cttgacgcat
      601 tacagaagtt tgatgtggaa caaattgatc ctcttggaca gacatttgac cctcaacaac
      661 atgaggcaat gtcaatgcaa cctgccccag gagcgccacc gaattctgta attactgtat
      721 tccaaaaagg ttacaaattg agcgatcgag taattcgacc agctcgggtg atagtttcca
      781 ccaaataatc tggtgtttca gttgaggtat ttttaaacaa atttttagat gcagtgtatt
      841 cagctttttt tagggtgata gcttgaaatc gtcatgatgc accccatatg tggaatattg
      901 catgagaaaa tattaatttg gagcaaaata gaatggctaa aataataggt atagacctgg
      961 gtactacgaa ctcttgtgtt gctgtcatgg aaggtgacaa accgaaggta attgaaaata
     1021 gtgaaggtca cagaacaact ccttctatcg tagcatttac tgatgataat gaaatattgg
     1081 taggccaatc agccaagcgt caatccgtga ctaacccaga gaaaacgctg tttgcgatta
     1141 aacgattaat tgggcgtcga tttgatgatc ctatcgtgca aaaagatatt aagatggttc
     1201 cctacaaaat tatgaaagcg gacaatgggg atgcttgggt tagagtcaaa gatcaagata
     1261 aggcaccacc acaaatttct gctgaagtgt taagaaaaat gaaaaaaaca gcagaagatt
     1321 atttgggtga agaagtaaaa gaagcagtga ttactgtacc tgcctatttc aacgattcgc
     1381 aacgccaggc aaccaaagat gctggtcgta ttgctggcct ggaagtaaaa cgaatcatta
     1441 atgagcctac cgctgctgca ctggcttatg gtatggataa aaaacgcggt gactctgtta
     1501 ttgcggtata tgatttaggg ggaggaactt ttgatatttc aattattgaa attgccgaag
     1561 tggatggtga acatcaattt gaagtacttg caaccaatgg tgataccttc ctcggtggag
     1621 aagattttga tttggcttta attgagtatt tggcttctga gttcaagaaa gacactggta
     1681 ttgatttgca taatgatcct cttgctctgc aacgcttgaa agaggctgcc gagaaagcaa
     1741 aaattgaatt gtcatctgca caacaaactg atgtcaattt gccttacatt acagcagatg
     1801 cttctgggcc aaaacattta aatatcaaat tgactcgcgc aaaactggaa tctttagtgg
     1861 agaaactggt tgagagaact attgagccat gtaaaacagc tttaaaagat gcagggctta
     1921 ctgtctcaca aatcaacgaa gttattctgg tcggtgggca aactcgtatg cctctggtac
     1981 aaaaaactgt ggaagaattc tttggcaaag aacctcgtaa ggatgtaaac cctgatgagg
     2041 ctgtggcagt aggtgctgca attcaggcag cagtattgtc cggtgaagtg aaagacattc
     2101 ttttactcga tgtcactcct ttgtctctcg gtatagaaac catgggtggt gtaatgacca
     2161 aactcattga aaaaaacaca actattccta caaaggctac acaagtgttt tcaacagccg
     2221 atgataatca gacagcagtg actgtgcatg tattgcaggg tgaaagagaa caggcttcag
     2281 caaacaagtc actgggacgg tttgatttgc gtgacatccc tcctgctcct cgtggtgttc
     2341 ctcaaattga agtaactttt gatattgatg ccaatggtat cttgaatgtt tctgccaaag
     2401 acaaggcaac tggcaaggca caatccattg tcatcaaagc ttccagtggt ttaagtgaag
     2461 aagaggttgc tgctatggtg aaagatgccc aatcgcatgc tgaagaagat aagaaattca
     2521 aggaaatggc agaactgcgc aatcaggcag atagtttgat tcatagctgt gaaaaatcta
     2581 tgaaagattt ggctgatgag ttgtctgagg atgagaaaag aggcatcgaa acagcaattt
     2641 ctgagctgaa agaggcggtg caaggaactg acaaggctcg tattgaagac aagctgaaag
     2701 tattgacaga cgcttccgca aaaatggctg aacgtattta tgctaaaaaa tcgtctgaag
     2761 gtcaggcagc tcaggggcaa acacaatctc aggaatctac aaaacctgcg gaagagggag
     2821 ttgttgatgc tgagtttgaa gaagttaaag aagaggataa aaagtaattt aatattactc
     2881 tcgccctctg atttaagagg gcggaagaag gattcatcgg gttataataa ataaattttg
     2941 ggtcggggcg atattatcgt cccgtttgtc tattgaaata gcacttaagc taatttagag
     3001 tcttcatacg actgagagaa ttaaaattaa gtgattgtta taaattgtga gcagttatgg
     3061 aacagcggga ttattatgaa cttttagaag taagtcgaaa tgcgagtgat gcagagatta
     3121 aaaaagcata tcgcaggctg gcaatgaaat accatcccga tcggaatcca ggtgatacct
     3181 ctgccgaaga aaaatttaaa gaaattcaaa aagcctataa catcttgtcc gataagcaaa
     3241 aacgcgccgc ttatgatcaa ttcggccatg ccggagtgga tccttcaatg ggtggaggcc
     3301 caggtggttt tggcggattt ggaggatttg gtgacgtttt tgaagatatt tttgagaata
     3361 ttttttcagg ggggcgtggg catgggcgtc aatcacgtgg acaaagaggc gctgatttgc
     3421 aatttaatgt gcaattgacc cttgaagaag cggctatagg aaaagaagtt gaaattaccg
     3481 ttccaagaca tggtacttgt actgtttgtg agggatcggg ggctaaaaaa ggcacaagcc
     3541 caaaaacttg tgaaacatgc cagggcatgg ggcaagtcag aattcaacaa ggtttttttt
     3601 ccatacaaca aacctgcccc acttgtcatg gtgagggaaa aataatttct gatccttgtg
     3661 ccagttgtca tggtcaagga agagttagag aaagtaaaaa aataaatgtc aaaatcccgg
     3721 ctggagtcga taatggtgat cgagtccgtt taagcggaga aggtgaagca ggggtgcatg
     3781 gtggtggttc aggagattta tatgttcaaa ttagcttgaa aaaacatgct atttttgagc
     3841 gccatgaaaa tgatttgcat tgtgaagtac ctattagttt tgcaactgcg gcgttaggag
     3901 gctcgatcga agtacccact ttggaaggcc gagtcacttt aaaaattccg gcggaaactc
     3961 aaacaggcaa agtatttcgc ttgagaagta aagggatgaa atctgtcaga ggatatggac
     4021 aaggcgattt gctatgcaag gtagttgtag aaacaccggt caatttatct cgagagcaaa
     4081 aagaactgtt aaataagtta caggattcat tagagaatgc caagggaacg cactcaccta
     4141 aaacaagctc ctggtttgcc ggtgtgaaaa aattcttcga agacatgaaa ttttgactta
     4201 attaagttac aattggaaga tttgtcgttt tcagcatgga ttgcctattt tacgctggat
     4261 aagcatactt tgaacataat tgagtatatt catgattaat aaatcagcaa ttttagcttt
     4321 agctgatggc actgtttatg agggcctgtc agtaggggcc agtggtgatt ctgttggtga
     4381 gttggttttt aacacgtcaa tgactggcta tcaggaaatg cttactgatc cttcgtatgc
     4441 cagacaaata attaccttaa ctactgctca tgtgggaaat accggttgta acatggagga
     4501 catggagtcc acaaaagtct gggcagccgg cctcgtcatg agaaactgca ctgcaatccc
     4561 tagcaattac agagctgaac aatccttccc agactggtta agtaaaaatg gagtagttgc
     4621 aatttctggc attgatacgc gcgcgcttac actgaaattg cgtgagaagg gttctatcgg
     4681 agcctgtatt agcaccaatg tggagaaacc tgaaattgtt gtggcaaagg ctaaatcctt
     4741 tgcagggctt cagggtgcag atttggcttt agaggttaca agacaaacga tagagcgttg
     4801 gcatgaaggt cggggaatgt ggagtctaga caaacagcct caacagcttc atgttgttgc
     4861 ctatgatttt ggtgtaaaac ataatatttt acgtatctta catgataagg gatgccatat
     4921 tactatagtc cctgctaaaa cttctgcaga agaggtttta gccatgaatc ctaatggtgt
     4981 ctttttatcc aatggaccgg gtgatcccca agcatgcgat tatgccatca aagcaacaca
     5041 ggtgttttta gacaataata ttcctttatt tggtatatgc cttgggtttc agattttggc
     5101 tttggcatgt ggtggtatta ctaaaaaaat gaaatttgga catcatggtt ccaatcatcc
     5161 tgtagttgaa acaactggca aaaaacgtgt ttttataaca agtcaaaatc atggttttgc
     5221 tgtagatgag gattcattgc ccaattgcct ggaagttacg catcgttcat tatttgataa
     5281 cagtttacag ggaataaggc acaaaacaaa accggcattt ggttttcaag gccatccaga
     5341 agcaagccct gggccacatg atatagatat tttatttaat gaatttttaa atctgatgaa
     5401 ataaattttt agcttcagaa ttattatttt ggcaagagtg gcttgtttta agcctgctta
     5461 caatgattgt aataccccta aatgctatcc atggctatta aagattagta aaatggcatg
     5521 gatttaccaa aacaatgact ttaacttgtt ttgtgtttta agcagagata aagattaggg
     5581 atactaaaaa tagaaaatac agaggatatg taatgaatga agtctatgtt tttacctcag
     5641 agtctgtttc cgagggccat cctgataaga tagctgatca aatttcagat gccattttag
     5701 atgctatttt agcccaagat ccaaaagcac gagtggcttg tgaggtattg gttaaaaccg
     5761 gcatggtttt ggttggtgga gaaattacga cgaaagcttg ggttgatgtt gaagaaataa
     5821 ctcgtcatgt tatcaaggac attggctata atagttccca gatggggttt gattgggaat
     5881 cttgtgctgt gctttcagcg attggcaagc aatcaccaga catagctcaa ggtgtcgata
     5941 atcagcaaac taaaattctg ggtgctggcg atcagggact tatgtttggt tatgccagcc
     6001 gtgaaacaga tgtttttatg cctgccccta ttgcctatgc gcatcgtttg atggagaaat
     6061 tagctaaagc tcgtaaatca ggccaattac cctggttaag acctgatgca aaatgtcaac
     6121 ttactttgaa atatgaacaa gggatgccgg ttgaagtgga tactgttgtt ttttctacgc
     6181 agcattcacc tgatattgag cataaagatt tggttgaggc gatccgtgaa gaaattatta
     6241 aatcagttct tcctgctgaa tggctcaatg ataagacccg gtatttcatt aatccgacag
     6301 gacgcttcgt aattggtggt cctttgggcg attgtggctt aaccggccgt aaaatcattg
     6361 ttgatactta tggtggtatg gcaagacatg gcggaggctg tttttcaggt aaagatcctt
     6421 cgaaggtaga tcgctccgca gcatacgccg caagacatgt tgccaagaat attgtggcgg
     6481 ctggtttggc ggataaatgc gagttacaga tttcctatgc tattggtgtt gctgaaccta
     6541 catctatttt tgttgatact tttggtactg gacgactaaa aaacagcgaa ataattgatt
     6601 taattcatac gcattttgat ttaacaccgc aaggtattat cgaccaacac gatttgcttc
     6661 gtcctattta taggcagacg gcaacttatg ggcactatgg cagggagagt tttccatggg
     6721 agcgattgga caaagtagct gaattaagca aggcattata attttttaag gatactctaa
     6781 tggaactggt agaagaagga gtacagaaaa tcaatacatt acaagattat aaagtagcag
     6841 agatttcatt agcggactgg gggcgaaaag aaatagcgat agctgaaacc gaaatgccgg
     6901 gtttaatggc tttacgcgag gaattttctg cgaaaaaacc acttcaaggc gctcggattg
     6961 caggttgtct gcatatgacg attcaaaccg cagtattgat tgagaccctg gttgcattgg
     7021 gtgctgaagt acgctggtct tcctgcaata ttttttctac tcaggatcat gcagcttccg
     7081 ctattgctgc aaaaggaatc cctgtttttg cctggaaggg cgaaacagaa gaagaatatt
     7141 ggtggtgtgt tgagcaaact ttaagtggcc ccaatggttg gacacctaat ttattattag
     7201 atgatggcgg ggatctgaca caagttgttc accagaaaca tcccaaatta ctgactgcaa
     7261 ttaagggagt atctgaggaa accactacag gtgtagccag gctgtacgaa atggcgaaac
     7321 atggacaatt aaaaattcca gccattaatg ttaataatgc ggtgaccaaa tccaaatttg
     7381 ataatttgta tggatgtcgg gaatccctgc ttgatgggtt aaaacgtgca acagatgtta
     7441 tgatagctgg taaggttgcc ttgattctag gctatggtga cgtagggaaa ggttgcgctc
     7501 aggccttgcg cggacaggga gccactgtac ttgtcgcaga aattgatcct atttgcgcac
     7561 ttcaggcagc aatggaaggc tacagagtag tcactctgga tgatgttgct gaacaagtgg
     7621 atatcgtagt tacagctact ggcaattacc atgttgtaac tcacgatcac atgaaacgta
     7681 tgcgtaatca ggcgatattg tgcaacatag gccattttga ttccgaaatt gatattcaaa
     7741 gcttaaaaca gtatcaatgg gaaaatatca agccgcaggt tgatcatgtg atttttcctg
     7801 atggaaagcg gattattatc ctggctgaag ggcgtttagt gaatttgggc tgcgctacag
     7861 gccatccgag ctttgtcatg tctgcttcct ttaccaatca agtattagcg cagattgagc
     7921 tgttccaaaa ctctagccaa taccagaatc aagtatatgt acttccaaaa gttttggatg
     7981 aaaaagtagc ccgtttgcat ttgggacgta ttggtgcaaa attaactcaa ttaagtaatg
     8041 agcaggctga ttatatcggc gttgataaaa atggacctta caagccggat cattatcgtt
     8101 attaaactaa ttttattcaa gatttagtca agataactaa atcttgaatt tttatacatt
     8161 attggatctt atactctaat atagattaga attaattcac ttatcactta aaaaagaaaa
     8221 gcctgttgat ggatctggtt atagcggcaa attatctttc tactctggaa aaatgtcttg
     8281 aacaatttgg gattaaccat tttttagaaa aaattggctt tgatctgata agagtacatg
     8341 atcctgatgc ctacttaact caggatgaat ttaaccaggt tattttggaa gcttataaat
     8401 taagcaattg tccggaattg ggtttggttt ttggtcaaaa cttatctata gtcaatcacg
     8461 gttttcttgg gtatgcagca atgagcagtc ctacatttgg aactgctata caaactgtgc
     8521 tcagttacct taatacacga accaatttgt tgtctctgga gttacgccag tttaatactg
     8581 aaaacagtgc ttatatcaag ttattaccac acacaaaaga aatggtttta tcacgtttta
     8641 taactgaatt ggcaattact catcttatta aaatgagaaa ttttttgatt agttgtaatg
     8701 acccattgct caagattgag ttaaattacg atgaacctcc ttatgcaaaa tattatcaaa
     8761 caatttttca gactaaaatt atttttaata cagaggctac tcgcgtttgg tttaaagcgg
     8821 aggaactgaa ttatcccatc cattttgctg acgatgtcag ttatcaactg gcaaaaaggc
     8881 aattgcaaga acttgaaaaa aaaatgtcat taaaagagga tatggcaagc aagattaaat
     8941 ctattttaat caatcagaat ttaaatcacg tcagcatgga tgagatagcc agtcgattat
     9001 gtatgacttc tcgtactcta agacgtcatt tgcagagatt ggatgtgact taccaagagt
     9061 tgcttgatga ggtaagagaa caaaaagcaa agacattttt gctcaataat aatatttcca
     9121 tgaccgagat ctgtttttta ttgggttttc aagatacatc caatttttca aaagcattca
     9181 aacgatggac aggaatcact cccacagaat atagggaaaa acatgaagtc caatgattgc
     9241 tcgattttaa tttaatctca tggcttattg attcttgagt tgatatttat tctcctgcat
     9301 actgaggtgg ttttattttt a
//
LOCUS       DACTJE010000126         9284 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.126, whole genome shotgun sequence.
ACCESSION   DACTJE010000126 DACTJE010000000
VERSION     DACTJE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9284
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.126"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..179
                     /locus_tag="I9760_002239"
     CDS             <1..179
                     /locus_tag="I9760_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HAT5044001.1"
                     /translation="ATLPFILSIVTKGVKLALLSDPHLLNGLNVHQGKITYEAVARDL
                     GYEYTPAAAALSGR"
     gene            complement(251..2635)
                     /locus_tag="I9760_002240"
     CDS             complement(251..2635)
                     /locus_tag="I9760_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HAT5044002.1"
                     /translation="MKKMAYFWRKGLWALMSLFFLLIVAGSLLYLYLESQLPNVDSLK
                     TVQLQVPLQIFSKEGLLIQEYGEKKRIPVTYDEIPPTLIHALIATEDQRFFEHPGVDV
                     MGLGRAAVSMLKTGTKSQGGSTITMQVARNFFLSRKKTFLRKFNEIMLAIKIDRELSK
                     EKILELYLNRVYLGNRAYGVGAAAMVYFGKSLKDLNLAELAMIAGLPQAPSTQNPIAN
                     PLAAKKRRDHVLERLLEEHYINEEQYQNAVNQPITAKYHGTNIKVKAPYVAEMIRQSL
                     YDNFGPEAYTKGYKVYTTIDGKLQNTANEVVEKNLIAYDHRHGYRGPIANIGEKDSQS
                     PKIRQKYLRQYPELNSLIPAVVTEVREKEATAALQNGQIIIIPWEGMSWARPALKRGG
                     WVGKSPTKAQQVVAAGDIIYVHPTEKSWQLAQIPEAESAMVAMNPKNGAIEVLVGGFN
                     FSKSKFNRATQSSRQPGSSFKPFVYAAALNNGYNLATLINDAPIVVDDPSQPNLWRPH
                     NVNLKFNGPTRLKQALVQSKNLVSIRILDDIGIDYTIDFLTRFGFNKKSLPRALSLAL
                     GSLSISPMDLTTAYAVFANGGYKVEPYLIDHITDADGKILLQAKPSVVCDPCDKDKVD
                     ASTLAPRVISEDIAFLMNTALRDVIQHGTGRAARVLNRQDIAGKTGTTNDQVDAWFAG
                     FNSNLVVTTWIGFDNPKSLHEYAANLALPLWIDFMKVALKDKPETELKQPENVIAVRI
                     DPVSGLLAKPNQENGIIEYFRQNEVPSEEDQGPVYNAHNDQQEEELAPVEESLF"
     gene            complement(2787..3794)
                     /locus_tag="I9760_002241"
     CDS             complement(2787..3794)
                     /locus_tag="I9760_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044003.1"
                     /translation="MTRLEELLYTLTSVIIRYHDSQPKVLNKLIVESNPILLRRKALA
                     LAKEIIQDKDIECKGRLEELIQNCTKGYPDRKPFLYFILNEVLFLKSMLDRKSSFEPS
                     KLEGYKKQIAQLLIDLKQLMITPKSKTYEVKYTSATENSETSISLSGVKNDGYVGGEF
                     CNSGNLIKEEVLEALNIHINSKNTAECSSDEEIKALAESLCLEHQNALLVPELSEKAR
                     ILTESSQEQENELVTKTKQIETDKKTILEQQATIQEKDKEIEALKEKISDLTRTAPRP
                     VFNPVLPMGYSSLFFNTRLGTQAKQQTTFDVTDLPIIKSSESKDAEPNKSFSMGNSHG
                     D"
     gene            4211..5275
                     /locus_tag="I9760_002242"
     CDS             4211..5275
                     /locus_tag="I9760_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HAT5044004.1"
                     /translation="MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEI
                     PASALEGNMIKDVDVVANCIKKLVERLKFTSKNVALAVPDSAVISKVVQINEGLTDLE
                     MEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
                     AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFS
                     REEKFGGMQLVEAIAEHYHMNLEQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTL
                     QFFYSTSQHGFVDHILLAGGMARQPGLASLIQEQLGVTTTVANPLSHMSFGKKVNLDV
                     INSDAPTLMVACGLALRHVE"
     gene            5279..5827
                     /locus_tag="I9760_002243"
     CDS             5279..5827
                     /locus_tag="I9760_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilN"
                     /protein_id="HAT5044005.1"
                     /translation="MTEINLLPWREQKREQEKKLFTTMLLIGIIIAAAIVFLMNSYVS
                     HLISNQTNRNQILQKEITALDEQIKEIKALKQIREGLISRMSIVQNLQSTRTLMVHLF
                     DELIKITPPGVYITKMQREHDVVTLWGYSESNTSISILMRNIEANVWIQNPVLTEIKK
                     NVEKKQSPNNEFKLSFVLKPKY"
     gene            5841..6437
                     /locus_tag="I9760_002244"
     CDS             5841..6437
                     /locus_tag="I9760_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HAT5044006.1"
                     /translation="MDTVNLNELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKP
                     NFEQLEIQEAQEIALKTEFEKKQHQAANLQAYRNQLQLMNERFGTMLKQLPAKNEMPG
                     LLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
                     IVTLHEFSVEGVSGDGKTVSQDELVMAITAKIYRYRAQ"
     gene            6434..7018
                     /locus_tag="I9760_002245"
     CDS             6434..7018
                     /locus_tag="I9760_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HAT5044007.1"
                     /translation="MIIKNNQTLILGFFGNSLCTLLTSENFRKSLLTALLVLLLSACS
                     GDNSDLIKYINEVKSRPGRPIEPIPKFAPLPIFRFPENDDRRNPFKPIDQKKRNDIYA
                     PDKKRPKQPLESFPLDALKFVGTLKKDNEIWALIKQPDGQISRVRVGDYMGQNYGRII
                     LIKNDLIKLEETVQKSGTWEKQSTTINLDTGKQE"
     gene            7022..9118
                     /gene="pilQ"
                     /locus_tag="I9760_002246"
     CDS             7022..9118
                     /gene="pilQ"
                     /locus_tag="I9760_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HAT5044008.1"
                     /translation="MRKIIAILILISASLSLAYSKDNSLISVKVLPLPENRVRIDFQF
                     SNPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKTKTIQLGSLHSYNIVAVGDRVRA
                     ILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
                     EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITM
                     QQEGKKVRMTILSKGDYGHFVYQVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLN
                     FQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQALDIILTTQGLDKRRK
                     GNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSL
                     LSDRGKISVDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQD
                     IGIRWGVSRPTHLSGTLEGANELARGTAPANVVPLERRLNLDLAAAPLTGATPASVGI
                     ALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQEATSSGA
                     TAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVN
                     NGQTIVLGGIYKQDKNKVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITN
                     ALSITTIEGREKDVYK"
BASE COUNT         2892 a         1614 c         1910 g         2868 t
ORIGIN      
        1 atgccacatt acctttcatt ttaagtattg ttactaaagg ggttaagctg gctttattaa
       61 gtgaccctca tttacttaat ggcctaaatg ttcaccaggg taaaatcact tatgaggctg
      121 ttgctcgtga tttaggatat gaatacacac cagctgcagc tgctttatca ggacgataat
      181 tagcggatta aaacccgtat tacacaaatt gaatacgggt tttgtgcttc atcagaatat
      241 aaatctcaaa ttaaaacaga ctttcttcca ctggcgctag ttcttcttcc tgctgatcat
      301 tatgtgcatt atagacagga ccttgatcct cttcagatgg aacctcattt tgtcgaaagt
      361 actcaatgat gccattttcc tggttaggct tggcgagcaa accacttacg ggatctattc
      421 gaacagctat aacattttca ggctgcttca attcagtttc aggtttgtct tttaatgcta
      481 ctttcataaa atcaatccat agcggaagag caagatttgc agcatattca tgcaatgatt
      541 tgggattatc aaaaccaatc caggttgtta ctaccaaatt agagttaaat ccggcaaacc
      601 acgcatctac ttgatcatta gtagtccctg ttttaccagc gatgtcttga cgatttaata
      661 ctcgtgcagc acgtccagtg ccatgctgta ttacatcgcg taatgccgta ttcattaaaa
      721 aggcaatgtc ttcagaaata acacgaggtg ccaaggtaga ggcatcaact ttgtctttat
      781 cacaagggtc acatacaaca cttggtttgg cttgtaacag aattttacca tcagcgtctg
      841 ttatatggtc aattaggtag ggctctactt tataaccacc attagcaaag actgcataag
      901 cagtggttaa atccataggg ctaatggaca aacttcctaa agcaagtgat aaggctctag
      961 gcaatgattt tttattaaag ccaaaacgag ttaagaaatc tatcgtataa tcaataccta
     1021 tatcatcaag aattcttata gataccaaat ttttagattg aactaatgcc tgtttcaggc
     1081 gggtagggcc attgaatttt agattcacat tgtgtggccg ccataaattc ggttggctgg
     1141 gatcgtctac aacgatgggg gcatcattaa ttaaggtagc aagattataa ccattgttca
     1201 aagccgcagc gtaaacaaat ggtttgaaac tggagcctgg ttgcctgctg gattgggtag
     1261 ctcggttgaa tttacttttt gagaagttaa atcctccgac caaaacttca atggcgccat
     1321 ttttaggatt catagcaacc attgcagatt ctgcttcagg aatttgggcg agttgccagg
     1381 atttttccgt ggggtgtaca taaattatat cgcctgctgc taccacttgt tgtgctttgg
     1441 ttggtgattt cccaacccag cctcctcttt tcagtgccgg cctggcccag gacataccct
     1501 cccagggaat aataattatt tgcccgtttt gtaatgccgc agtcgcttct ttttctctta
     1561 cttcagtgac cactgctgga atcagactgt tcagctctgg atattgccgc aagtattttt
     1621 gcctaatctt gggagattga ctgtcttttt cacctatatt ggcaattggc cctctgtaac
     1681 catgtcgatg atcgtaagca attagatttt tttctaccac ttcattggca gtattttgta
     1741 atttgccatc aatggttgta tataccttat agcctttagt gtacgcttcg gggccaaaat
     1801 tgtcgtacag agactgacga atcatttcag caacataagg cgctttcacc ttgatattag
     1861 ttccatggta ttttgcagta attggttgat tgactgcatt ttggtattgt tcttcattga
     1921 tataatgttc ttccagtaat cgctccagta catggtcacg acgttttttt gcagcaagcg
     1981 ggttagcgat aggattctga gttgagggag cttgaggaag tcctgctatc atagccagtt
     2041 cagccagatt aaggtcttta agggattttc caaaataaac catggctgcc gcgccaacac
     2101 cgtaagcccg attacccaga taaaccctgt ttaagtaaag ttccaaaatc ttttctttgc
     2161 ttaattcccg gtcaattttg atagcaagca taatttcatt gaatttccgt aaaaatgttt
     2221 ttttacggct taagaaaaaa ttacgagcta cttgcatggt gatcgtactg ccaccttgag
     2281 atttggttcc agttttgagc atgcttactg cggctcggcc caatcccata acgtctacac
     2341 cagggtgctc aaaaaatcgt tgatcttctg ttgcaattaa tgcatggatc agagtagggg
     2401 gaatttcgtc ataagttacg ggaatacgtt ttttttcacc gtactcctga attaataaac
     2461 cttctttgct gaaaatctgt aatggcactt gtaattgtac agtttttaat gaatcaacat
     2521 taggcaattg gctttcgaga taaaggtata gaaggctgcc agccactatt aggagaaaaa
     2581 acaggctcat cagcgcccaa agacctttac gccagaagta tgccattttt ttcatagaat
     2641 ttcgttttcg ttaaatgtga gtagtgcaat attatacagc gaatctatat aatattgcga
     2701 ggtttacaag ggaaaaaaac ctgatttaaa tgctattttg cgcctcacag ctatggagat
     2761 aatcatcgat gaagcgccaa tagactttaa tctccatggc tattacccat agaaaacgat
     2821 ttgttaggct cagcgtcttt tgattctgag gacttaatta ttggtaaatc agttacatcg
     2881 aaagttgttt gttgtttagc ttgagtaccc aatctggtat tgaaaaacag agagctgtat
     2941 cccataggta agactggatt gaaaacgggg cggggtgcag tgcgcgttaa atcgcttatt
     3001 ttctctttca gtgcttctat ttctttgtct ttctcctgaa ttgtagcttg ttgttctaaa
     3061 attgtttttt tatctgtttc aatttgtttt gtttttgtta ccagttcgtt ttcctgttct
     3121 tggctgcttt cagtcaatat tcgggccttc tccgataatt caggtaccaa gagcgcattt
     3181 tgatgctcta aacataaaga ttcagcaagc gctttaattt cttcatcaga agaacattct
     3241 gctgtatttt tagaattaat atggatgttc aatgcttcta aaacttcttc tttgatcaaa
     3301 tttccggagt tgcaaaattc tccaccaacg tatccatcat tttttacgcc tgaaagagaa
     3361 atgctggttt cagagttctc agtagcagaa gtatatttta cttcataagt tttactttta
     3421 ggggtgatca ttaattgttt gagatcaatt aaaagctgag cgatctgttt tttatatcct
     3481 tccagtttag atggctcaaa tgaacttttc ctatccagca tggattttag aaaaagaact
     3541 tcatttaaaa taaaatataa gaatggtttt ctgtcagggt aaccttttgt gcaattttgt
     3601 attaattcct caaggcgtcc cttacattca atgtctttat cctgaataat ttctttagcc
     3661 aaagcaagtg cttttcttct taagagaatg ggattgcttt caactattaa tttatttaat
     3721 accttaggct ggctgtcatg ataccgaatg attacagaag tcagtgtata gagtagctct
     3781 tctaatctag tcatatgtaa agctcctttt tataaagcta aaaatctaaa tgtggaaatc
     3841 ccctacaact tggtatttca ctatattgaa tgttttgcat aagtgcaagt gcttgttaac
     3901 tggtacatta tttgaaaaaa acatacaagt agaggggttg ttttacagtt ttttgaaaga
     3961 atataaaatc tgatttgatt gtaatactat gattgatatc atgaaatttg cttgggcatc
     4021 gacagaagac agaaatggaa atgcatggat caattgtcag attgattata aacgcttaac
     4081 ccaaagtaat aagtaaagtg attccaattt atagtggctt acgctaacat taattattca
     4141 atagttagga aaagatctaa aaataggagt gcttttgggc tgcaaggaaa tatacaggga
     4201 gattatataa gtgctcaaat tgcttaaacc taagcatcgt tcaattcttg gcattgacat
     4261 cagttcttcc gcagtgaaaa ttctggaaat atcaggttcc ggagaggaca tttgtgttga
     4321 aggatatggt cgtgaagaaa tacctgcctc tgccctagaa ggaaatatga tcaaggatgt
     4381 cgatgtagta gcgaattgta tcaaaaaatt agtagaaaga ttgaagttta catctaaaaa
     4441 tgtggcattg gctgtacctg attcggctgt tatcagtaaa gtagtgcaaa ttaatgaagg
     4501 gttaactgac cttgaaatgg aagagcttgt cgttattgag gcggataagt acattcctta
     4561 cccaatagat gaaataaacc tggattttga agtgttaggc cattcagcca agagctcatc
     4621 actgcttgat gttttaatcg tggcatcaag ggcagaaaac gttaataccc gtgttgaagc
     4681 agcccataga gcagggctgg aacccaaagt tgttgacgtt gaatcttatg cagtagagcg
     4741 ttcggtacaa caaattgcgc aagaattacc agcaagtggg caagacaaaa ttgttgcaat
     4801 agtagatatt ggagccaatt atactcactt atttgttttg catggtatgc gactaatctt
     4861 ttcaagagaa gaaaaatttg gaggtatgca acttgttgaa gctattgcag aacactatca
     4921 tatgaatttg gagcaggcta ttgccctaaa aaatcagggt aaactaccgg aggactacga
     4981 atctgctgta ttagagcctt ttaaggacat gattcttttg cagatcaaac gtactttgca
     5041 gtttttttat tcaaccagcc aacatggatt tgtagaccat attttattgg cgggaggaat
     5101 ggcaagacaa ccaggattgg catcgcttat tcaagaacag ttaggagtga ctactacagt
     5161 ggcaaatcca ctatcccata tgtcttttgg taaaaaagtg aatttggatg tgattaacag
     5221 tgatgcgcct acactgatgg tggcttgtgg gttggctttg aggcatgtcg agtaaaaaat
     5281 gactgaaatt aatttattgc catggcgtga acaaaaacgt gagcaggaaa agaaattatt
     5341 tacaaccatg ctattgatag ggattatcat cgctgcagca attgtttttt taatgaactc
     5401 ttatgtctct catttaataa gtaatcaaac gaaccgaaat cagattttac aaaaagaaat
     5461 tacagcttta gatgaacaga tcaaggagat taaagcatta aaacaaatca gggaaggatt
     5521 aatttcaaga atgtcgattg tgcagaatct gcagtcaaca agaactttaa tggttcactt
     5581 atttgatgag ttaataaaaa ttactcctcc aggagtttac ataaccaaga tgcaaagaga
     5641 acacgatgtg gtaacacttt gggggtattc cgaatcgaat acaagtattt ctattttgat
     5701 gagaaatata gaggccaatg tctggataca gaatcctgtt ctaactgaaa ttaaaaaaaa
     5761 tgttgaaaaa aaacagtctc caaacaatga atttaaatta agttttgtgc ttaaacccaa
     5821 atattaggct gataaaaatc atggacacgg tcaatttaaa tgaattaact ctagagaatg
     5881 ttgggcagtg gccaatgccg gttaaaatcg gagtaatttt aggcatcagt atactgataa
     5941 ttggattagg atattggctt attgttaagc ccaattttga gcaattggaa atacaggaag
     6001 cccaggaaat tgctttgaaa acagagtttg aaaaaaagca acatcaggca gcaaatctac
     6061 aagcctatcg taatcagcta cagttaatga atgaacgctt tgggactatg ctaaaacaac
     6121 ttccggcgaa aaatgaaatg cctgggcttt tggaggaaat atctaaaacg gggatcgcat
     6181 cgggtttgaa atttgaattg tttgcaccac aacctgaggt agtgcatgat ttttatgtgg
     6241 agttacctat aaaaattacg attgtcggat cctatatgca attggcaaga tttttaagtc
     6301 gtgtagctga aatgaacaga atagtgactt tgcatgaatt tagtgttgag ggggtgtccg
     6361 gggatggtaa aacggtctct caggatgaat tggtgatggc tatcactgcg aaaatataca
     6421 gatatcgggc acaatgataa ttaaaaataa ccagactctt atattaggct ttttcggaaa
     6481 ttctctgtgc actttactca cctcagagaa tttccgaaaa agtctattga cggcgttatt
     6541 ggttctatta cttagtgcct gctctggtga taacagtgac cttattaagt acatcaacga
     6601 agtgaaatca agaccaggga gacctatcga gcctattccc aaattcgccc ctttgccaat
     6661 ttttaggttt ccagaaaacg acgataggcg aaatccattc aagcctattg atcaaaaaaa
     6721 gagaaatgat atttatgccc cagataaaaa aaggcctaag cagcctttgg aatcttttcc
     6781 tctggatgct ttaaaatttg taggtacttt aaaaaaagac aatgaaatct gggctctgat
     6841 taagcaacct gacggccaga ttagccgtgt tagggttgga gattatatgg gacaaaatta
     6901 tggacgtatt attttgatta aaaatgatct cattaaattg gaagaaactg tacagaaatc
     6961 aggaacgtgg gaaaaacagt ccaccacaat taatttggat actggcaagc aggagtgagg
     7021 cgtgagaaaa attatcgcga ttttaatatt aattagtgcc agtttgagtc tggcatattc
     7081 taaagataat tcgctcattt cagtaaaagt attgccattg ccagaaaaca gagtacgtat
     7141 tgattttcaa ttttccaacc ccttaaagca gttgcccgca agctttatta ctcaaaagcc
     7201 gcctcgtttg gtgttagatt ttatcagcac tgatatgcaa attccttcgg atcagaaaac
     7261 taaaactatt caattaggct cattacacag ttataacatt gttgctgttg gtgatagagt
     7321 aagagccata ctggaactgg atcgttcggt gtcctattct ggcagtacgg cgggctccgt
     7381 ttatagcctt gtcctgaatg gtaaaagtaa tgatttattt gaaaatagca aggaggtatt
     7441 cataacgaac caggttgtga acgccaagca tgaaattaaa agaattgatt ttcgtggcat
     7501 agaaaagcag ggaggtagag ttttaattga tgtttctgat acgggtattc caattgatgt
     7561 aactcaagtt gggaaagaaa tagtcgttaa tttcctgaat accaagatcc ctttgaattt
     7621 aatgaaacgg tatgatgttt ctgattttca cagccctgtg cagattatta cgatgcaaca
     7681 ggaaggcaaa aaggttcgca tgaccatcct cagcaaaggg gattatgggc attttgttta
     7741 tcaggtcaat aaacaattta tggtcgatgt ctttccttta actgcggaag aaattcgtca
     7801 ggcgaaattg aagaaacagg tatttaccgg taaacggatt tctttgaatt tccagaatat
     7861 tagtattcga gctgtgcttc aactactggc agattttacc ggaatcaata tggttgtcag
     7921 tgacaaagtg caaggagata ttacgcttag attaaatgat atcccctggg atcaggctct
     7981 ggatatcatt ttaaccacac aagggctaga taagcgcaga aagggcaacg tcatgttgat
     8041 agataccaaa gcctccatgg ataaaatgga ggaagaacaa ctcaaaagtc aacagacaat
     8101 tgaaaaactt gagcctatcc gctctgattt aatacagatt aattatgcaa aggcagcaga
     8161 tattgctgtt ttgatcaagg ataagcaaaa ctccctatta tcagacagag gaaaaataag
     8221 tgttgatgcc agaacgaata caatctggat tcaggatact ggcaccaaaa ttgaggaagt
     8281 aagggaactg ataaagcaat tggatatccc tgttaagcaa gtgcttattg aggcaagaat
     8341 tgttgaagta actaaagatt ttagccagga tattggaata cgctgggggg tatcaagacc
     8401 aactcactta agtggtacac tggaaggtgc taatgagtta gcgagaggca cagcaccagc
     8461 caatgttgtc cccttggaaa gaaggttaaa tcttgatttg gcagctgctc ctttaactgg
     8521 agctacaccg gcctcggtag gtattgcgct agctaaactt aatgataaca ttctgcttga
     8581 tctggagttg tctgctttgg aaagcgaagg gcgtgctgaa ttaatatcca gtcctcgcct
     8641 gattactacg aatcaacagg cagcagtcat agagtctggt gaggagattc cctatcagga
     8701 ggcaacatca agtggagcga ctgctgttgc atttaaaaaa gcagtattga gtttgaaagt
     8761 tacgccgcaa attacgcctg atggtaaaat tttgatggat ttacagataa atcaggatac
     8821 tccttcccca caaacattcc ttggtgttcc agcaataata acaaaggaga ttcagacgaa
     8881 tgttctggtt aataatggac aaaccatagt acttggaggt atttataagc aagataagaa
     8941 caaggtaatc aatagaattc ctttctttgg tgagttgcct gttgtcggta ttttatttag
     9001 taatagacaa atcacattga aaaatgaaga attacttatt tttattacac caagaataat
     9061 aacgaatgcc ttatcgatta caacgataga gggacgtgag aaagatgttt ataaataatc
     9121 aatattggac taattttatc tcattaataa agataaaaaa cattaattta atagaagtgc
     9181 ctcaacacgg tgaacatgtt ataattaaaa tatagcccgc tatgatgtaa tggcattttg
     9241 aaaaaaattg ctcatcagcg atggattggg taaagttgaa gtat
//
LOCUS       DACTJE010000127         9140 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.127, whole genome shotgun sequence.
ACCESSION   DACTJE010000127 DACTJE010000000
VERSION     DACTJE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9140
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.127"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(65..1312)
                     /locus_tag="I9760_002247"
     CDS             complement(65..1312)
                     /locus_tag="I9760_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAT5044009.1"
                     /translation="MVTQFNTLKSYIFPIILLLSILLGGLTGYVFNQNVAWLKPFGDI
                     FLNLIFTTIVPLIFFSVSSAIARTGSIGKLGKIFYSMTVIFLFTGIVAAVYALFVVLL
                     FPPAQNMPVLLESSKQLSPTNLPDRLIEIFTVPEFSKLFSHEHILALVLFSILVGLAV
                     AHSKDKGTIFNSFLKAGEDVFMSVFRLIMYYAPIGFFAYFAVIVSEIGPQLMENYLRI
                     AILYYTASVFYFFFALTAYAYLAGKSQGVRLFWGNIFLPAATSIATCSSAASIPANLM
                     ATKSMRIPAEIYETSIPLGSIIHKDGSVMGGVFKIAFLFGIFHLPFTSLPVLMMALGV
                     SLLVGTVMGAIPSGGMLGELLILSVYGFPPSVLIAVAAISIIIDPIATMVNVTSNSVC
                     SMMIARLVEGKNWFLRSSDENNL"
     gene            complement(1434..4199)
                     /locus_tag="I9760_002248"
     CDS             complement(1434..4199)
                     /locus_tag="I9760_002248"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAT5044010.1"
                     /translation="MDKTYSPEAIEKALYKKWESHHYFQPRGEGKRFCIMLPPPNVTG
                     SLHMGHGFQHTIMDALTRYHRMLGDKTLWQPGTDHAGISTQLVVERQLEAQGISRKDL
                     TREQFLDKVWQWKEESGNTITQQMRRLGASVDWSRERFTMDEGLSAAVQKVFVQLYEE
                     GLIYRGTRLVNWDPKLGTAVSDLEVLSEEEDGFLWHIRYPVVDSEEFLIVATTRPETL
                     LGDCAVAVHPDDSRFRHLIGKQVHLPLCDRTIPVIADDYVDKEFGSGCVKITPAHDFN
                     DHEVGKRHQLPQINILTKKGTINKNAPLKYQGMDRFVAREQIIKDLEQEGLLAKTEPH
                     KLKVPRGEKSNVIIEPLLTDQWYVKTKPLAEPAIAAVKKGDIRFIPETWDKTYFQWMD
                     NIEDWCISRQLWWGHRIPAWYDNHGNIYVGYSENDVRFKHKIDQSTPLKQDEDVLDTW
                     FSSALWPFSTLGWPERTPELEQFYPTSVLVTGFDIIFFWVARMIMMGLKFTGKIPFKE
                     VFITGLIRDSEGHKMSKSKGNVLDPLDIVDGIDLDSLIAKRTSNLMLNSVRDRITKAT
                     RKEFPEGISAYGTDALRFTYCSLASTGRNVRFDLGRVEGYRNFCNKLWNAARYVLLNT
                     DEEQIDFGDGAFQYSPADQWILSRLQNTVSKVHHYFETYRFDLLANTLYEFVWHEYCD
                     WYLELSKPILQDEQALSAMKRGTRRTLIHVLDQILKLLHPLMPFITEEIWQKTTKFTS
                     ENGISIMLSTYPKVNEEFINPAIEEELDWLKSAIQSLRTIRSEMSISPAKLIPLYIRN
                     ITPELKERIAKYEKILKTLSKIDKINYLAPDEKVPVSATAVLGEIELLIPMADLIDKE
                     AELSRLNKELAKLNKDIELAQGKLNNPKFTDKAPEEIIAKEKDKLAQAQLAKDKLLQH
                     KNRIESL"
     gene            complement(4455..7502)
                     /locus_tag="I9760_002249"
     CDS             complement(4455..7502)
                     /locus_tag="I9760_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HAT5044011.1"
                     /translation="MKFTDLFIKRPVLSIVVSMLIFLFGINSIYKMQIRQYPKMENTV
                     ITIMTGYPGADAELIAGFITTPLESAVASAEGIDYMTSSSQQGLSTITLNIKLNFDPQ
                     VAFTDVMSKVQQTLNQLPKESQQPVILKKSDSSTALMYISLDSKDMTPQQITDYATRV
                     VQPQLQTVDGVAKADILGGQTYSMRIFMDPIKMAALNVTPADVSSVLASNNFLTAAGS
                     TKSEYVAINVTAKTDLNNVGEFERLIVKSDKGSIIRLRDIAKVELGSQNYDSSVTFNS
                     KKAVFISITPTPTANPLTVINDARKLFPSIVREFPPSLTGTIVYDATAFIRASIHEVM
                     ETIIEAAVIVIIVIFLFLGSVRSVLIPVVTIPLSLVGVCTLMLILGYSINLLTLLAFV
                     LAIGLVVDDAIVVVENVHRHLEEGKTPFESAIIGAREIATPVIAMTITLAAVYAPIGF
                     MSGLTGALFKEFAFTLASAVIISGIIALTLSPMMCSKILSEEATSGKFAIFLDEFFNK
                     LKRVYQNALHSILDTREIMLLLAGVVILMLPYLYSNTAAETAPDEDQGFFFVLATAPQ
                     YATLNYIEAFTKPFDAIYKSFPETENYFTVNTSQPISGMVLKPWDQRKKSQFALKQPL
                     QNKLSKIAGLNALAIVPPPLPGGGGGPAVQFVVKTTNDFQSLMDVSNQLTEKARNSGL
                     FIYIDNSLKFNQPQVEFSINRAKASEMGLDMRALGSTLTSALSNNYINYFNLEGRSYQ
                     VIPQLARKFRLTIEQIGQIYVKTLNGTMVPLSTVITPTEKTQPNAATHFQQLNSATIQ
                     AVMMPGKTLGQGLEFLQKAAQEVLPKGFTYDYGGQSRQFMQEGSALIFAFLFAIIVIF
                     LVLAAQYESFRDPLIVLISVPMSICGALIPLNLGAATINIYTQVGLITLIGLISKHGI
                     LIVDFANHLQREKNLDKRAAVEEAAGIRLRPILMTTAAMVFGVIPLLIASGAGAASRF
                     DIGLVISTGLLIGTCFTLFVVPTLYTYLAEDHRHKPNEPAEIQ"
     gene            complement(7619..8881)
                     /locus_tag="I9760_002250"
     CDS             complement(7619..8881)
                     /locus_tag="I9760_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAT5044012.1"
                     /translation="MNKRLTIMGIILLVVFGGIIAFNLIKAYMIKQFFASYAPPAVTV
                     SSAVAQAVDWKPTIDAVGNFVAINGVDVNSEASGNVVQIHFESGQYVEKDEPLITIDD
                     SIDQALLKFNQSELTLKELSYKRQTDLFKRGATPSSSVDEAKANLQQAQAKVEQIQAQ
                     IKQKHIGAPFSGRLGIRQVNLGQYVTPGQTSIVSLQSLDPLYLEFYLPEQYYKRIHLN
                     QPVTFRVEEFPNILFEGVIHAVNSKVDLTTHNVQVQALLPNCPADAIQDPTKSPLIKV
                     RKQVRGDKLIVSCSTELNKQNKIRNFVFIPGMFASIEVDQPAEPNTIIVPSTAISYSL
                     YGNAVYIIEKSKEGKKNKDGTDQLVVNRVFVTTGEQQGNYTVIKKGIKVGQLVVSTGD
                     LKLQNGTPVVINNSVKLDTDSDPNQLGQ"
BASE COUNT         2707 a         1791 c         1858 g         2784 t
ORIGIN      
        1 ttatattgca catatgggat aatgaaaatt taatatgtgc tttttatatt aaacatacct
       61 tagattatag gttattttca tcggaggatc tgagaaacca atttttacct tcgaccagac
      121 gtgcaatcat catgctgcaa acactattgc ttgttacgtt gaccattgtc gctatgggat
      181 caatgatgat gctaatagca gcaactgcaa ttaaaacaga aggtggaaaa ccatacactg
      241 acaaaattaa caattctcct aacatcccgc cactaggaat cgctcccatt actgttccta
      301 ccaacaaaga aacccccaat gccatcataa gaacgggcaa gctggtaaaa ggtaaatgaa
      361 agatcccgaa taaaaaggca attttaaaaa caccgcccat gactgatcca tctttatgaa
      421 ttatggagcc taagggtatt gatgtttcat agatttcagc agggatgcgc atggatttgg
      481 ttgccattaa gttggcagga atactggcgg cactactaca agttgctatg gaggtcgctg
      541 caggaaggaa aatatttccc cagaatagcc ggacaccctg ggattttccc gccaaataag
      601 cataggcagt taaggcgaag aaaaagtaga acactgaagc ggtatagtag agtatggcaa
      661 tgcgtaaata attttccatt aattgggggc caatttcgct tactattact gcaaaatagg
      721 caaaaaaacc gataggagca taatacataa tcaggcgaaa tactgacatg aatacgtctt
      781 ctcctgcttt taagaaggaa ttaaaaattg tacctttatc tttggaatgg gctacagcca
      841 atcctaccag aattgaaaaa agaactaatg ccagaatatg ctcatgagag aataattttg
      901 aaaactcagg tacagtaaaa atctctatca agcgatccgg gagatttgtc ggagagagtt
      961 gctttgaact ttccaagaga acaggcatgt tttgcgctgg agggaaaagt aaaactacaa
     1021 aaagagcata aactgcagca actattcctg taaataagaa tatgactgtc attgagtaga
     1081 atattttgcc taatttgcca atagaaccag ttcttgcaat cgctgaggat acactgaaaa
     1141 aaattagggg aacgatggta gtaaaaatca aattaagaaa aatatcgcca aagggtttta
     1201 accaggcaac gttttgatta aaaacatatc ctgttaaccc accaagtaaa atagataata
     1261 gcaaaatgat ggggaaaata taggatttaa gcgtattgaa ttgagttacc acgagaagac
     1321 atcctttctt ttaaataatc cggtttgtgc catttgttcc ttacagtcac gtatagaata
     1381 atgagttaag ttgagccatg actgtaagga gggtttaact aaggatcatt gagttataat
     1441 gactcaatcc tgtttttatg ttgcaaaagt ttatctttag ccagttgagc ctgagctaat
     1501 ttgtcttttt ctttagcaat gatttcttca ggcgctttat cagtgaattt agggttattt
     1561 aatttaccct gagctagctc gatgtcttta ttcaatttgg caagctcttt attcagtctt
     1621 gataactctg cctccttatc aatcaaatct gccattggaa tcaacaactc aatttcaccc
     1681 aataccgcag tagctgaaac aggaactttc tcatcaggag caagataatt aattttgtct
     1741 atcttgctta gcgtcttgag aattttttca tattttgcga tacgctcctt taattcagga
     1801 gtaatattgc gtatgtataa aggtattaac ttggcgggtg agatagacat ttcactgcgt
     1861 atggttcgta gtgattgaat ggctgacttt aaccaatcca attcttcctc tatagcgggg
     1921 tttatgaatt cctcattgac tttgggatat gtgctcagca tgatactaat cccattttca
     1981 ctggtaaatt tggtggtttt ttgccaaatc tcttctgtaa taaatggcat cagaggatga
     2041 agaagcttca atatttgatc cagaacatga attaaagttc ttcgcgtgcc tcttttcatg
     2101 gcgcttaaag cctgttcatc ctgtaatatg ggcttggata attctaaata ccagtcacaa
     2161 tattcatgcc agacaaattc atatagcgta ttagcgagga gatcaaaccg ataggtttca
     2221 aaatagtgat gtaccttact tacggtattt tgtagccggg atagtatcca ttgatcagct
     2281 gggctgtatt gaaatgcgcc atccccaaaa tcaatttgct cttcgtctgt atttaataag
     2341 acatagcgag ctgcgttcca tagtttattg caaaaatttc tatatccttc aactcgtcct
     2401 aaatcaaaac gcacattacg ccctgttgaa gcaagagaac agtaagtgaa tcgcagcgcg
     2461 tctgttccat aagcgctgat tccctcagga aattccttac gagtggcttt tgtgattctg
     2521 tcacgaacag aatttaacat caggttagac gttcgtttgg ctattaaaga atccagatca
     2581 attccgtcta caatgtccaa tggatccagt acatttcctt tggatttgga catcttgtgc
     2641 ccttcactgt cacgaattaa accagttata aagacttctt taaagggaat tttcccagtg
     2701 aactttagtc ccatcatgat catacgggca acccagaaaa atatgatatc aaatccggtt
     2761 accagtacag aagtagggta aaattgttcc aattcgggag tacgctcagg ccagcctaat
     2821 gttgaaaagg gccagagagc tgatgaaaac caggtatcca gaacatcttc atcttgtttc
     2881 aggggagtag attgatcaat tttatgttta aagcgaacat cgttttcact ataacctaca
     2941 taaatattac catgattatc ataccacgca ggaattctat gtccccacca tagttggcgg
     3001 ctgatacacc agtcctcaat attatccatc cactggaaat aggttttatc ccatgtctcg
     3061 ggaataaaac ggatgtctcc tttctttaca gcggcgatag caggttctgc caatggtttg
     3121 gttttaacat accattgatc agttaataaa ggttcgatga tgacattcga tttttcacct
     3181 cggggtactt ttaatttgtg aggttctgtt ttcgccagca agccttcctg ttccaaatct
     3241 ttaatgattt gttctcgggc aacaaatcta tccatcccct gatattttaa gggagcgttt
     3301 ttattgatag tccctttctt tgttaaaata ttgatttgtg gtaattgatg acgtttgcct
     3361 acttcatgat cgttaaaatc atgtgcgggg gtaattttta cacacccact gccaaattcc
     3421 ttgtcaacgt aatcgtccgc aatcacgggt attgttctat cacataaggg cagatgaacc
     3481 tgctttccaa taaggtgcct gaaacgtgag tcatcggggt gaacggcaac ggcacaatca
     3541 ccgagtaagg tttcggggcg tgtagtggca actattagaa attcttcgga atcaacaaca
     3601 ggatagcgaa tgtgccaaag aaagccatct tcttcttcag agagaacttc aagatctgaa
     3661 acagcagtgc ccagtttggg gtcccaattc accaggcggg ttcctcggta aataagtcct
     3721 tcttcataaa gctgtacaaa taccttttgt acagcagcag acaatccttc atccatggtg
     3781 aaacgttctc gactccaatc tacggaagca cctaaacgcc tcatttgttg cgtgatggta
     3841 ttaccggact cttctttcca ttgccacact ttatccagaa attgttcacg ggtgagatct
     3901 tttctcgata tgccttgggc ttccagttga cgttctacaa caagttgagt tgagatgccg
     3961 gcatgatcgg tacctggttg ccataatgtt ttgtcaccaa gcatgcgatg atatctggtt
     4021 aatgcatcca ttatggtgtg ttgaaaacca tgtcccatat ggaggcttcc tgttacatta
     4081 ggaggcggaa gcataataca aaatcgttta ccctcaccgc gtggttgaaa gtaatgatga
     4141 ctttcccatt ttttataaag ggctttttct attgcttccg gagagtaagt tttgtccatt
     4201 atcgtatagc ctatactggt aaaaactcac aattttatca tagagtatgg gaatttagcc
     4261 attttaaaaa aacaggaact caggaaaaaa tcattatagt cctttgagtt ttaaatggcc
     4321 gattctctaa tgaccgttgc ttgtcgctgg tatccacaaa gtgcggctgc atgcttcgga
     4381 gaagaggaaa catgcaggct gtttgacgaa ttatttaaag attaataagg cttacgaaaa
     4441 aatccaaagt cgaattactg gatctctgct ggttcgtttg gcttatgtcg atgatcttca
     4501 gctaagtagg tatataaagt aggtactacg aacagtgtga aacaggtacc tattaataac
     4561 cctgtggaga taacgagccc tatgtcgaat cgactcgctg ctccagcacc actggcaata
     4621 agaagaggta ttactccaaa caccatggca gctgtagtca ttaatatggg tcgtaatcta
     4681 attccagcag cttcctcaac ggcagcacgt ttatcaagat ttttttcacg ttgcaaatga
     4741 ttagcgaaat ctactatcaa tattccatgt ttactgatga gtccaatcaa agtaatcaaa
     4801 cccacttgag tgtagatatt aattgtggct gctcccaagt tgagcgggat aagcgcacca
     4861 cagatagaca ttggcacgct gatgagaacg attaatggat ccctaaaact ttcatactgc
     4921 gctgccagca ctaaaaagat aacaataata gcaaaaagga atgcaaatat cagcgcactt
     4981 ccttcttgca taaattgcct ggactgtcct ccataatcat atgtaaatcc tttgggcaag
     5041 acttcctggg ctgctttttg taaaaactcc aatccttgac ccaatgtctt gcctggcatc
     5101 atgaccgcct gaattgttgc tgaattgagt tgttggaaat gagtcgctgc attaggttgc
     5161 gttttttcgg ttggggttat cacagtagat aatggaacca tcgttccgtt aagtgttttt
     5221 acataaattt ggcctatctg ttcaatagta agacggaatt tgcgagccag ttgtgggatg
     5281 acctggtagc ttcggccttc cagattaaaa taattgatgt agttgttaga tagagcactt
     5341 gtcagggtgc ttccaagcgc tctcatatcc aagcccattt cagaagcttt tgctcgattg
     5401 atgctaaatt ccacttgtgg ttggttaaat ttaagagaat tgtctatgta aataaatagc
     5461 ccactgttcc gggctttttc agttagctgg ttcgacacat ccattaaact ctggaaatca
     5521 ttcgttgtct taaccacaaa ctgaacggca ggtccaccac caccgccagg caggggaggt
     5581 ggaacaatgg ctaaagcatt aagtcctgca attttagata acttgttttg taaaggttgt
     5641 tttagagcaa actgactttt tttcctttga tcccatggtt tcaaaaccat tcctgaaata
     5701 ggttggctcg tgtttacagt aaaatagttt tcggtttcag gaaaactttt ataaatggca
     5761 tcaaaaggtt ttgtaaaggc ttcgatatag tttagggtag cgtattgtgg tgcagtagcc
     5821 agtacaaaaa agaagccttg atcttcatcc ggggcggttt cagcggctgt gttcgaatac
     5881 aaatagggca acattaaaat cacgaccccg gctaatagca acattatttc tcgagtatcc
     5941 aatatgctat gaagtgcatt ctggtaaacg cgtttcagtt tgttaaaaaa ctcatccaga
     6001 aaaatagcaa attttccact agtggcttct tcggatagaa tttttgaaca catcatgggg
     6061 gacagcgtta aggcaataat ccctgaaata ataaccgcac ttgccagcgt aaaagcaaat
     6121 tccttgaaca aagcgccagt cagtccactc ataaacccta ttggtgcata aacagctgcc
     6181 agggtgattg tcatcgcaat aaccggggta gcaatttctc tggcgcctat aattgcggat
     6241 tcaaaggggg tttttccttc ttccaaatgg cgatgtacgt tttctaccac tacaatagca
     6301 tcatccacta ccaatcctat tgctaacaca aaggcaagaa gggtaagcaa gttgatgctg
     6361 taaccgagta tgagcatcaa agtacagaca ccaaccaatg acagcggaat ggtgacaacg
     6421 ggaataagaa ctgaacgaac agagcctaaa aataaaaaga taacgatgat cacgatgact
     6481 gcggcttcaa tgatagtttc cattacctca tggatagatg cccgaatgaa tgctgttgca
     6541 tcgtatacta ttgtgccagt taatgacggc gggaattctc tgactatcga aggaaacaat
     6601 tttctagcat cattgattac tgtgagaggg ttagccgttg gtgttggagt gatcgatata
     6661 aagacggcct ttttgctgtt gaaagtcact gagctatcat aattttgtga acccagttcc
     6721 actttagcga tatccctgag cctgattatt gaccccttat cgctttttac aatcagtctt
     6781 tcaaattcgc cgacattgtt caaatcagtt ttagccgtga cattgatggc tacgtattca
     6841 cttttagtac ttccagccgc agtcaagaaa ttgttacttg ctaaaacaga ggacacatca
     6901 gcaggagtga cgttaagcgc agccatttta attggatcca tgaatattcg catggaataa
     6961 gtttgaccac ctaaaatatc agccttggct actccatcca ctgtttgcaa ctggggttga
     7021 acgactcggg tagcgtagtc agtaatttgc tgaggagtca tatccttact gtccagacta
     7081 atgtacatca atgcggttga tgagtctgat tttttcaata tcaccggttg ttgtgactct
     7141 ttaggtaact gattgagcgt ttgctgcact ttactcatga catcagtaaa agcaacttga
     7201 gggtcgaaat tcaacttgat gtttaaggta attgtgctaa ggccttgctg actggatgaa
     7261 gtcatataat ctattccttc agcactggca acagcacttt caagaggagt agtgataaat
     7321 ccggctatga gttcagcatc agcgccagga taaccagtca ttatagttat gactgtattc
     7381 tccattttag ggtattggcg aatttgcatt ttatagatgg agtttatccc gaatagaaaa
     7441 atcaacatac tgaccactat ggagagtacc gggcgcttga tgaatagatc tgtgaatttc
     7501 ataagttatc ctgcaaaaaa ctgaccatga tcctagtcgg tagtaaagaa caacaagcca
     7561 tattattaga gcatgccaat gttataaaat gatttaaata ccaggttgcc aacacgatct
     7621 actgtcccag ttggttggga tcactgtctg tatcgagttt gacactgttg ttaataacaa
     7681 caggggtacc attttgtaat ttcaagtcac cagtacttac gaccaattga cctactttaa
     7741 tgcctttctt gataactgtg taatttccct gttgctcccc ggtagtgacg aatacacggt
     7801 ttacaaccaa ctggtcagta ccatctttat ttttcttacc ttctttactt ttttcaatga
     7861 tataaacagc atttccatag aggctgtaag aaatggctgt ggaagggacg ataatagtat
     7921 taggctctgc aggttgatcc acttcaatgg aagcaaacat gcctgggata aatacaaaat
     7981 ttcgtatttt attttgtttg tttaattctg tactgcagga cacaatcagt ttatctcctc
     8041 tgacttgttt acgtactttg ataagaggag atttggttgg atcctgaatt gcatcagcag
     8101 ggcagttcgg taatagggct tgtacctgaa cattatgagt agttagatct actttagagt
     8161 ttactgcatg aatgactcct tcaaagagaa tattaggaaa ttcctctacg cgaaaggtca
     8221 caggctggtt aagatgaatt cttttgtaat attgctctgg caagtaaaat tccaggtaaa
     8281 ggggatccag tgattgcaaa gaaactatgg aggtttgtcc tggagtgacg tattggccca
     8341 ggtttacctg gcggatgcca aggcggccgg aaaaaggggc tcctatgtgt ttttgtttaa
     8401 tttgcgcttg aatctgttct actttcgcct gagcctgttg caagttagct ttggcttcat
     8461 caacactgga gcttggcgtg gcgccacgct tgaagagatc agtttgccgt ttatagctga
     8521 gttctttaag cgttaattcc gactggttaa atttgagcaa ggcttgatcg atgctgtcgt
     8581 caatggtgat caagggctca tctttttcta cgtattgccc tgattcaaaa tgaatttgaa
     8641 ctacattgcc agatgcttcc gagttgacat caactccgtt gattgcaacg aaattaccta
     8701 cagcgtcaat ggttggcttc caatccactg cttgagcaac agcagaagaa acggtaacag
     8761 caggaggcgc ataactggcg aagaattgtt taatcatgta agccttaatt agattaaaag
     8821 ctatgattcc accgaaaact accaacaaga ttattcccat gatagtcaat cgtttattca
     8881 ttatcttccc ctgttaatgc ttgaaaaaaa ttttaattta gcaaatataa cataaacatt
     8941 tactttatca tcttattgtt tgagatgact tgttattttt aatattttca tagttgtttc
     9001 tgcttcttct ctaacaaaat tgaattgtaa tgaactttga ttttatgggt acaaaaaaat
     9061 cctattgact aaattgaata ctttgatact atgcagcttc catcttacta aggggaagct
     9121 catgaagtta aaatcattgt
//
LOCUS       DACTJE010000128         9096 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.128, whole genome shotgun sequence.
ACCESSION   DACTJE010000128 DACTJE010000000
VERSION     DACTJE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.128"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(11..1459)
                     /gene="ravH"
                     /locus_tag="I9760_002251"
     CDS             complement(11..1459)
                     /gene="ravH"
                     /locus_tag="I9760_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavH"
                     /protein_id="HAT5044013.1"
                     /translation="MDKEQGAYMPPKDGKELLDIIDNLNEFAEKLLSRIADESDEGCK
                     QLVHVLESARARYNKPIESWLKGWFYQFTRKRGQEIDMAIKGMEAFPDAYTRLQEFKL
                     LVGKGEWEHGSFNYYLFDELIKSVPGYKPLESELVHPIILKLRQKINKRIDEFMSQYL
                     ATQKLIEARKQELLQTHQSAQKFIDNVSITNDLEAAKKSAKENKGVIQFCLIFESDIW
                     KLSWVAATGKVYALEPGEELIQILIDGKIQDIVKDIDNLSALHLKQLKQECVKAREML
                     LGRVQLLINPKDPRTQAELTNDVLKENGTSATFVLRGKPNEYSLWWINTLGFAHEISL
                     ESYASMKTWLNDQSHPLKEEHIPQLKTYLLNVNTVKSIDVKEDMKEFKAQLQECLAAG
                     PKSKTPPASGATKTTNAKKLDLRLFGDVERCLRSQLSERQEEKAAIQEPGKINLNKYE
                     NIATLFGNRAKNMEQHEGQQSKPNDSLSIRSI"
     gene            1625..2263
                     /locus_tag="I9760_002252"
     CDS             1625..2263
                     /locus_tag="I9760_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HAT5044014.1"
                     /translation="MRITLLTTAILATGVASAAVPVDGWYASAFGGYTYIPPNIYNFA
                     YGVFRDEVGYNNGYNAGGRIGYQSNPIRYEVEYTYLRGEARKFNLNFITQLGVTGFTS
                     ANTIMANIYYDCPDMLPAIAPFIGLGIGYASLRTELDSIGPFRPSYFTTSDSAFAYQG
                     TVGITYNFSENYAANLAYRYIATDNSSNFGKTYQAHLASVGAIYRFDQGNYK"
     gene            2273..3037
                     /locus_tag="I9760_002253"
     CDS             2273..3037
                     /locus_tag="I9760_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044015.1"
                     /translation="MKKLTQLSVLSGLLLSGSTFAFNQPQGFYVGILGEISHGPSSED
                     IAFLYEDRNTYTGTVKYSSVSGGGGAVLGYKISHYRIEGEVLYNRISTGPLTINTCTL
                     QSPNVFTPTGVCPPGVPPSGYDNFRVNGTGYDGSSAALYGLLNLYYDFFSSVHESTYV
                     PYIGLGAGQVRIQNRSNFVKTVLGRLPPSFGTSSTMVSGAGQGIIGVGYLMDDFAWLA
                     MDFRFLTTNSLSDFNNKRYTLSTFNVGVNFTFDKST"
     gene            complement(3029..4081)
                     /locus_tag="I9760_002254"
     CDS             complement(3029..4081)
                     /locus_tag="I9760_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAT5044016.1"
                     /translation="MNENHKELISIGLTVSIVIFSLYIVHKFIPSMIWAAIIVIATYP
                     LYTRWRKLFGNKHNTSAFLFTTLMGLLFLIPLSWLIGILIKESQLFINFLQHINKEGG
                     AAPEFFKNIPLVGDDLIQYWDVNIGKPGSIKEFLSNLHVTLTPTSYYIKQIGVNLAHR
                     SFQVGFTLLTLFFFYRDGDKLLLQIQHIGEYCLGDRWFRYSDRLPSALRGTVNGTIVV
                     GIGVGILMGICYALVGFPAPTLTGFITALAAMIPFVVPIVFIIVALILLSVGSLIGGI
                     IVLVWGTLVMFVADHFVKPVLIGGAIQLPFLAVLFGILGGVETLGLLGLFLGPMVMVL
                     FVTLWQEPQGHKKSSG"
     gene            complement(4081..4563)
                     /locus_tag="I9760_002255"
     CDS             complement(4081..4563)
                     /locus_tag="I9760_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAT5044017.1"
                     /translation="MNQVNLANRVWQDPVYFIAFGFGSGLMSIAPGTWGTLAAIPLYL
                     LMINAHWSIYLLWTALAFALGVWVSDRVTEDLGIHDYKGIVWDEVVGYLLTMFLAPKG
                     ISWMLIGFILFRIFDIWKPQPIRMIDQRVHGGLGIMLDDVLAAVPAWCIMQILVWSFA
                     "
     gene            complement(4653..5609)
                     /gene="thiL"
                     /locus_tag="I9760_002256"
     CDS             complement(4653..5609)
                     /gene="thiL"
                     /locus_tag="I9760_002256"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAT5044018.1"
                     /translation="MNEFTLIDHYFKSIPCKRNDVLFGIGDDAACLQIPDGMHLLVST
                     DTLVSGIHFLPQWDPYDIACKAVMVNISDMAAMGAQPCWATLALTLPELNQSWLDSFA
                     RGIKDSLGLYNVDLIGGDTTKGPLSITLTIMGLTPQGQAIRRCGAQAGDVIMVSGELG
                     AAGLAVKLLDGHKKVNAKDKIELMNKLLHPKPRVDLCHMLRKYATSAIDISDGLGADL
                     NHICVASGVGANLFEEAIPRHPLLNQYCPEQALDLALSGGDDYELCFTVSTQQLDSFN
                     RDLKKENLICFPIGVIEEKAGLRIQSADSPSKELQPRGYSHF"
     gene            complement(5602..6045)
                     /gene="nusB"
                     /locus_tag="I9760_002257"
     CDS             complement(5602..6045)
                     /gene="nusB"
                     /locus_tag="I9760_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAT5044019.1"
                     /translation="MEKQSIRGKRRARKFALQALYQWLMSGTDLHEIEAQFRTINNMD
                     KVDGEYFCRLLYGIPTHVEALEASLLPYLDREINALNPIELTVLRIGSFELFHCPEIP
                     YKVILDESVSLTKEFGSQEGYRYVNGVLNNLAKQVRSVEVSLDNE"
     gene            complement(6103..6570)
                     /gene="nrdR"
                     /locus_tag="I9760_002258"
     CDS             complement(6103..6570)
                     /gene="nrdR"
                     /locus_tag="I9760_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAT5044020.1"
                     /translation="MYCPFCHAEETKVVDSRLVADGAQVRRRRECLECHERFTTFETA
                     ELIMPLIIKRDGRREPFHIDNLRSGMLRALEKRPVSVDDLEKAIISITEEIRRRGERE
                     IDSQVVGELVMKELFRLDHVAYVRFASVYKRFKDVSDFRQTIDQMKNEDKEKS"
     gene            complement(6573..7826)
                     /locus_tag="I9760_002259"
     CDS             complement(6573..7826)
                     /locus_tag="I9760_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAT5044021.1"
                     /translation="MFDESYTIKNFDDVLFKAISDEKRRQEEHIELIASENYVSPRVL
                     EAQGSVLTNKYAEGYPGKRYYGGCEFVDVAEELAISRAKLLFGAHYVNVQPHSGSQAN
                     AAVMMALLSPGDTFMGMALPHGGHLTHGSKVNFSGKLYHSVEYGVDSNTGLIDYDALE
                     KLALQHKPKLIIAGFSAYSRILDWARFREIADKVGAYLMADIAHVAGLVAVGLYPSPV
                     PYADVVTTTTHKTLRGPRGGLILCKENEEIEKKLNSSVFPGMQGGPLMHVIAAKAVAF
                     AEALLPEFKTYQQQVLANARTMCSVLQSRGYDIVSGGTDNHLLLVDLINKGITGKEAD
                     AALGRANITVNKNSVPNDPRSPFVTSGLRLGTPAATTRGFKEREITLLSNWVADVLDN
                     VHDETNISRVKTQVLLLCREFPVYA"
     gene            complement(7854..8165)
                     /locus_tag="I9760_002260"
     CDS             complement(7854..8165)
                     /locus_tag="I9760_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HAT5044022.1"
                     /translation="MRDSLKMLLVGSISMIIIACAASPLSESTGEYLDSSTTTAKVKA
                     SLVDQLGTTGFAVKVKTYKDQVQLSGFVDSQKIKQRAGIIAAGVDGVKSVRNDLIIKT
                     R"
     gene            complement(8290..8727)
                     /locus_tag="I9760_002261"
     CDS             complement(8290..8727)
                     /locus_tag="I9760_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044023.1"
                     /translation="MKYLIPCVVLFPLLSSCTVIQEDFYQPEYLGPAPRVEVTPSYPQ
                     GHYHRHYNQRYHSNNRYYSAPRGKVHYEHAKTQGHAAVVTPPPSQARVEVQKNVHGHN
                     ANNGQVHGHAHDNGNVHSHPDSSNVHGHDTSNPEEQKKIHGHS"
     gene            complement(8793..>9096)
                     /locus_tag="I9760_002262"
     CDS             complement(8793..>9096)
                     /locus_tag="I9760_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044024.1"
                     /translation="KSIQKSVTYPGYCQIEIINQSFTDVRVFGTFDDGTTIDFNIYRY
                     ESPHYISLFYNFYCHSGMYITITSPYYTIYSGWTNVNSTIRVVPYLKEAKAELSTR"
BASE COUNT         2761 a         2003 c         1634 g         2698 t
ORIGIN      
        1 catttattaa ttaaatagat cttatagaga gactatcatt tggttttgat tgttgtcctt
       61 cgtgttgctc catgtttttc gctctgttac cgaacaaggt ggcaatattc tcatatttat
      121 ttagattaat tttaccaggt tcttgtattg cagccttttc ttcttgcctt tcacttaact
      181 ggcttctcaa acaccgttca acgtctccaa ataagcgcaa atccagtttt ttggcattcg
      241 tcgtcttggt tgcgccactg gcaggaggtg ttttagattt tggtccggca gccagacatt
      301 cctgcaattg agctttaaat tccttcatgt cttccttgac atcaatggac ttgacagtat
      361 ttacattcag taaataagtc ttaagttgag gaatgtgctc ttcctttaac ggatgacttt
      421 gatcatttaa ccatgttttc atagatgcat aggattctaa tgaaatttca tgtgcgaaac
      481 ctaatgtatt tatccaccac aatgaatatt cattaggttt accgcgcaaa acaaaagtag
      541 cagaagtccc attttctttt aatacatcat tagtcaactc agcttgagtt ctcggatctt
      601 tcggattgat gagcagttga actcgtccta aaagcatttc tcgggctttt acacactcct
      661 gtttaagctg cttcaaatgc aaagcactta gattatcaat atccttaacg atatcctgaa
      721 tctttccgtc tattagaatt tggattaact cttctccggg ttccaaagca tagactttgc
      781 ctgttgcagc tacccaggaa agcttccaaa tatcactttc aaaaattaag caaaattgta
      841 taacaccctt attttctttg gctgattttt tagctgcttc caggtcattg gtaattgaaa
      901 cattatcgat aaatttctga gcactttgat gtgtctgtaa caattcctgt tttcgagctt
      961 ctattaattt ttgggtagcc aggtattgcg acataaactc gtcaattctt ttatttattt
     1021 tttgtctgag cttcaaaata attggatgga ctaactcact ttctaatggt ttatagccag
     1081 gaactgactt gataagctca tcaaataaat aataattaaa cgatccatgt tcccactccc
     1141 ctttcccaac caataactta aactcctgca agcgagtgta ggcatcaggg aacgcttcca
     1201 tgcctttaat tgccatatca atttcttgtc cgcgttttct ggtaaactgg taaaaccaac
     1261 ctttaagcca tgattcaatg ggcttgttat atcttgcccg ggcagattcc aagacatgaa
     1321 ctaattgctt acatccttca tcggattcat ccgcaattcg acttaataac ttttcggcaa
     1381 attcgtttaa attgtcgatt atatccaata actcttttcc atcctttggt ggcatatacg
     1441 ctccttgttc cttgtccaaa tttcatctta ccttaatcag aattttatac aagcaacaaa
     1501 atcattgagc taactggaat ttaaaattta aattgctcaa taaagcgcaa tagaaaactt
     1561 gcaaatttaa gacaaaccgc taacatgaat cagttaaaaa ataataataa taaaaaggga
     1621 tgtcatgaga attacattac ttacaacagc tatattagct acgggtgttg cctccgccgc
     1681 ggtacctgta gacgggtggt atgccagcgc atttggaggt tacacttata ttccccccaa
     1741 catatacaat ttcgcttatg gggtattccg tgatgaggtg ggatacaata acgggtataa
     1801 cgccggagga cgaataggct accaaagcaa ccccatccgc tacgaagtag aatacaccta
     1861 tctcaggggt gaagccagaa agtttaatct taactttatc acacagctcg gtgttaccgg
     1921 ttttacctcc gccaatacta tcatggccaa tatttactat gattgccctg atatgctgcc
     1981 agccattgcg ccatttattg gattggggat tggttatgcc tctcttagga cagagcttga
     2041 cagcataggt ccttttagac catcttactt taccaccagc gacagcgcct ttgcctacca
     2101 gggaactgtg ggaataactt ataatttctc cgaaaattat gcggcaaatt tggcttaccg
     2161 ctatatcgca accgataatt cgagcaattt tggaaaaacc taccaggctc atttggccag
     2221 cgtgggggca atctaccgtt ttgatcaagg aaattataag taaggatgcg caatgaagaa
     2281 gttaactcaa ctctcggtac tatccggttt gctattaagc ggttcaactt ttgctttcaa
     2341 ccagcctcaa ggcttctacg ttggaatatt gggggaaata agtcatgggc catctagtga
     2401 ggatatcgcg tttctttatg aagatcgaaa tacatatacg ggaacggtca aatatagttc
     2461 tgtttctgga ggaggagggg ctgtattagg ttataaaata agtcattacc gtattgaagg
     2521 ggaggtactt tataaccgga tttcaactgg ccctctaaca ataaatacgt gtactttaca
     2581 aagcccgaat gtttttactc ctactggagt ttgccctcct ggagtacccc caagtggata
     2641 tgacaatttc cgggtaaatg gcacaggtta tgatggcagt agcgccgctc tttatggcct
     2701 gctcaacctg tattatgatt ttttcagctc agtgcatgaa agcacttatg tcccttacat
     2761 tggtttagga gcaggacaag tacgcattca aaacagaagc aattttgtaa aaacagtatt
     2821 aggtcgcctg ccgccctctt ttggtactag cagtaccatg gttagcggag caggacaggg
     2881 cattataggg gttgggtatc tcatggatga tttcgcctgg cttgctatgg atttccgctt
     2941 tctcacgacg aatagcctgt ctgacttcaa caacaaacga tatacactca gcacattcaa
     3001 tgtgggtgtt aactttacct ttgacaagtc aacctgatga cttcttgtgg ccttggggtt
     3061 cctgccacaa ggtcacaaac aaaaccatca ccatcggccc tagaaacagg ccaagcaaac
     3121 ccaaagtttc tactcccccc aaaatgccga acaaaacagc caggaaaggt aattgtatcg
     3181 ctccaccgat aagaactggc tttacaaaat gatcagcaac aaacataacc aaagttcccc
     3241 aaaccagaac aatgatgcct ccaatcaaac tgccaacgga aagtaaaatc aaagcgacta
     3301 tgataaaaac aataggaaca acaaaaggta tcatagctgc caatgcggta ataaacccag
     3361 tcaatgtggg ggctggaaac ccaaccaagg catagcaaat gcccattaat atacctaccc
     3421 ctatgccaac aacaatagtt ccattcacag tacctcgcaa cgcgctgggg agcctgtcgg
     3481 aataacggaa ccatctgtca cccagacaat attctcctat atgctgaatt tgtaatagta
     3541 atttatcccc atcacgatag aagaaaaaaa gtgttaaaag ggtaaatcct acttgaaaac
     3601 tacgatgagc caaattgacc ccaatctgct tgatgtaata gctggtaggg gttaacgtta
     3661 catgcaaatt agataaaaac tccttgatac ttcccggctt accaatattc acatcccaat
     3721 attgaattaa atcatcccca accaatggaa tatttttaaa aaactcagga gcggctccac
     3781 cttccttatt aatatgctgc aaaaaattaa taaataattg agactccttg ataaggattc
     3841 ctataagcca actcaaggga ataaggaaaa gcaagcccat cagtgttgta aataagaacg
     3901 ctgatgtgtt gtgtttgtta ccaaataatt tacgccatct ggtgtataag ggataggtag
     3961 ctataacaat aatagcggcc catatcatgg aaggaatgaa tttatgaaca atataaagcg
     4021 agaagataac gatactgact gttaacccta tgctaattag ctctttatga ttttcattca
     4081 ttaagcaaag ctccaaacca ggatttgcat aatacaccag gcaggaactg ctgctaaaac
     4141 atcgtccagc ataatcccta acccaccgtg aactctttga tcgatcatac gtattggttg
     4201 gggtttccaa atatcaaaaa tacggaacaa aataaagcca attagcatcc atgatattcc
     4261 tttgggagct aaaaacatag ttaataaata accaactact tcatcccata caattccttt
     4321 gtaatcatgt atcccaagat cttctgtcac tctgtcactg acccaaactc ccagcgcaaa
     4381 tgccagggca gtccataata aataaatgct ccaatgagca ttgatcatta ataaatagag
     4441 gggaatagca gctaatgtcc cccacgtgcc tggagcaatt gacatcaatc cactgccaaa
     4501 accaaaagcg ataaaataaa cgggatcttg ccatactcga tttgccaaat tgacttggtt
     4561 catatttgct cctcaaccta aatatcgcta tcacatcact ttagaatcga attttgtctt
     4621 taatttttat tatcatacta tagaggcggg atttaaaaat gtgaatagcc tctgggttgc
     4681 aattctttac ttggactatc agctgattgt attcttaatc ctgctttctc ctctatcaca
     4741 ccgatgggaa aacagatcag attttctttt ttaagatccc gattaaaaga atccagctgt
     4801 tgtgttgaaa ctgtaaaaca taactcgtaa tcatcacctc ctgaaagagc caagtcaagg
     4861 gcttgttcag gacaatattg attcaataaa ggatgcctgg gtattgcttc ttcaaataag
     4921 ttggccccaa caccacttgc gacgcaaata tgatttaaat cagcgcctaa cccatcagag
     4981 atatcaatcg ctgaagtagc atattttcta agcatatggc ataaatccac ccgtggttta
     5041 ggatgtaata gtttattcat taattctatt ttatcttttg cattcacttt tttatgccca
     5101 tccagcaatt ttacagccag acccgcagcc cccaattcac cggaaaccat aatcacgtca
     5161 cccgcttgtg cgccacaacg tctgattgct tgtccctgag gtgtgagccc catgatagtc
     5221 aaagtgattg ataacgggcc tttggtcgtg tcaccaccga tcagatccac attatataag
     5281 ccaagagagt cttttatccc tctcgcgaag gaatcaagcc aactttgatt caattcgggc
     5341 aaggtcaatg caagagtcgc ccaacatggc tgtgcgccca tggcagccat gtcactaata
     5401 ttgaccatta ctgccttgca ggcaatatca tagggatccc attgcggtag aaaatgaatg
     5461 cctgaaacca aagtatccgt gctgactagc aaatgcattc catcgggaat ctgcaagcaa
     5521 gcggcatcat cccctatccc aaaaaggaca tcatttctct tgcatgggat ggatttaaaa
     5581 taatgatcaa tcagggtaaa ttcattcatt atcaagactg acttcaacag agcgaacctg
     5641 ttttgccaaa ttattcaata ccccattaac gtaacgatat ccctcttgtg aaccaaactc
     5701 tttagtcaag gatactgatt catcaagaat gaccttataa ggtatctcag ggcaatgaaa
     5761 caattcaaaa gacccaattc tcaatacagt aagctctatt ggattcaatg cattgatttc
     5821 tctgtctaaa tacggcagta aacttgcctc caaagcttcc acatgagttg gaatgccata
     5881 taacaggcgg cagaaatatt ctccatctac tttatccata ttattaattg ttctgaattg
     5941 agcttcaatt tcatgaagat ctgtaccaga cattagccat tgataaaggg cttgcaatgc
     6001 aaattttcga gctcgtcttt taccacgaat tgattgtttt tccacaatta cctcatatta
     6061 ttctaataac aacaagaaaa gtgagccctt cacatttttt atctaagact tttccttatc
     6121 ttcatttttc atttgatcaa ttgtttgtct aaaatcactc acatctttaa agcgtttata
     6181 cactgatgca aaacgcacat aggccacatg atcaagcctg aataattcct tcattaccaa
     6241 ctcgccaacc acctgggaat cgatctcgcg ttcacctctt cttcgaattt cctcagtgat
     6301 agaaataata gctttttcca aatcatcaac gctgacaggt cgtttctcca aggctctcaa
     6361 catgccagaa cgtaaattat caatatgaaa cggttctctt cttccatcac gttttataat
     6421 caatggcata atcaattcag cggtttcaaa agtagtaaac cgctcatgac attccagaca
     6481 ttcacgcctt ctgcgaactt gagcaccatc cgcgaccaga cgtgaatcca ctacctttgt
     6541 ttcttccgca tgacaaaatg gacaatacat gattaggcat aaaccggaaa ttcacgacag
     6601 agtagcagca cctgggtttt cactcttgaa atattagttt catcatgaac attatcaagg
     6661 acatccgcta cccagttaga taacaaagta atctctcttt ctttaaagcc tcttgtggtt
     6721 gcagcaggcg tacccaaacg taaaccactc gttacaaaag gtgagcgagg atcgttggga
     6781 accgaatttt tattgactgt tatgttggct ctgcctaaag cagcgtcagc ttctttacct
     6841 gtaattcctt tatttatcaa atccactagc aaaagatggt tatccgttcc gccagaaaca
     6901 atatcatagc ctctgctttg taacacactg cacatcgttc tggcattagc taaaacctgt
     6961 tgctgataag ttttgaattc gggtaacaga gcttctgcaa aagcaactgc cttggctgca
     7021 attacatgca ttaagggccc accttgcata ccaggaaata cagaagaatt taattttttc
     7081 tcaatttctt cgttttcctt acaaaggatt aaaccaccgc gaggtcctct taaagtttta
     7141 tgagttgtcg tcgtcacaac atccgcataa ggaacaggag aaggatataa ccctacagct
     7201 accaaaccag ctacatgagc gatgtccgcc attaaataag cgccgacctt atcggcaatt
     7261 tctctaaatc gagcccaatc caggatcctg gaataagctg aaaaaccggc aatgatcaat
     7321 tttggtttgt gttgaagcgc aagtttctcc agagcatcat aatcaatcaa acccgtattg
     7381 ctatcaacac catactcgac agaatggtat aatttacctg aaaaattaac ctttgaccca
     7441 tgagtcaagt gtccaccatg aggtaaagcc atgcccataa aagtatcgcc tggggacaat
     7501 aatgccatca taactgctgc gtttgcttgt gatcccgaat ggggttgcac attgacataa
     7561 tgagcgccaa aaagtaattt tgcccttgaa atggctaact cttcagcgac atcaacaaac
     7621 tcacaacctc cgtaatagcg tttgcctgga tacccttcag cgtatttatt tgtcaacaca
     7681 gacccctgag cctccaatac tctcgggctg acataatttt cagatgctat taattcaatg
     7741 tgttcttctt ggcgtcgttt ttcatcagaa attgctttaa aaagaacatc atcaaaattt
     7801 tttatagtat aactttcgtc aaacatgaat gttccttggt tggaaggtta tatttacctt
     7861 gtttttataa taaggtcatt acgaacactc tttacaccat ctacgccagc cgcaataatc
     7921 ccggctcttt gttttatttt ttgagaatcg acaaaaccgc tcaactgcac ttgatcttta
     7981 tatgttttta cttttacggc aaatcccgta gtccccaatt ggtcaaccaa actggctttc
     8041 accttggcag tagtcgttga actatcaaga tattcccctg tactttccga cagaggagaa
     8101 gcggcacacg caataattat catggatatg gatcccacta ataacatctt aagtgagtcg
     8161 cgcatagagg ctccttaaag ataaaaacgt tattagagta tcataaaaat tacccggata
     8221 cgaagaggat aatagtacaa ttttagttct tttaaaaaaa aagtttcctg tattcagcaa
     8281 gttaaattat taactatggc catgaatttt tttctgttcc tcaggattgg aagtatcatg
     8341 tccatgtaca ttagaagaat cagggtggct gtgcacatta ccattatcat gagcatgtcc
     8401 atgcacctga ccgttatttg cattgtgtcc gtgaacattt ttctgaactt caactctggc
     8461 ttgtgatggt ggcggagtaa ctaccgcagc atggccttga gttttcgcat gctcataatg
     8521 aaccttcccc cttggcgcac tgtagtatcg attattacta tgatatcgct gattgtaatg
     8581 acgatgataa tgcccttgag gataagaggg ggtaacctct acgcgaggag caggccccaa
     8641 atactctggc tgataaaagt cttcttgtat aactgtgcag gaagaaagta agggaaacaa
     8701 tactacgcag ggaatgaggt atttcatgta caactcctca aaggaaagga aaactctcga
     8761 atcattgaga gctttcccga attttcaatg aattatctgg ttgataactc agccttagct
     8821 tctttcaagt aaggaactac acggatggta ctgttcacat ttgtccaacc ggaataaata
     8881 gtatagtaag gtgatgtaat agtaatgtac ataccactat ggcaatagaa attatagaat
     8941 aggcttatgt agtgaggtga ctcataacga tagatattaa aatctatagt tgtgccatca
     9001 tcaaacgtac cgaatacacg aacatctgtg aagctttggt taatgatttc aatttggcag
     9061 taaccaggat aagttacgct cttttgtatt gatttc
//
LOCUS       DACTJE010000129         9028 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.129, whole genome shotgun sequence.
ACCESSION   DACTJE010000129 DACTJE010000000
VERSION     DACTJE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.129"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..756)
                     /locus_tag="I9760_002263"
     CDS             complement(<1..756)
                     /locus_tag="I9760_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT5044025.1"
                     /translation="MNIKSNLAYSSVILKGHVNRYAFLGLLISVGSILIASCIVSYQL
                     TGSVSLGGFIQAQRSNPAIWILDLTPFMFVYWGQAFCYGLVNKAESLLVDKTKELLNI
                     SGNLELKLKYDSLTNLPNNRLLNEKIRLAIEQLGSQGELAVIVIKINDLNYNFSSFNT
                     NNIVKQFAEKLKSVLIDPYMLKASLGINMVARLQSDEFAILMPRLKKDLEINELLIHL
                     LTLLNSNLMVDGININLATTVGAAIYPIHGEEDD"
     gene            954..1116
                     /gene="ssrS"
                     /locus_tag="I9760_002264"
     ncRNA           954..1116
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="I9760_002264"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1111..3102)
                     /gene="uvrB"
                     /locus_tag="I9760_002265"
     CDS             complement(1111..3102)
                     /gene="uvrB"
                     /locus_tag="I9760_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAT5044026.1"
                     /translation="MKDLFKIYSNYQPAGDQPTAIASLIDGLESGLAKQTLLGVTGSG
                     KTFTIAHVIQAMKRPTLIMAPNKTLAAQLYGEFKAFFPDNAVEYFVSYYDYYQPEAYV
                     PASDTFIEKDASINEHIEQMRLSATKALIERKDAIIVATVSAIYGLGDPDSYLRMLLH
                     LSRGEQSDQRKILKRLAEMQYTRTNLSLERGQFRVHGDVIDIFPADSEKEAIRIELFD
                     DEVDNIARFDPLTGEILQRLPRVTIFPKTHYVTPRERILETVEKVKVELQERLAELNA
                     QNKLVEAQRLEQRTCFDIEMMLELGYCSGIENYSRYLSNREAGEAPPTLFDYLPPEAL
                     LIIDESHVTVPQIGGMYRGDRARKETLVNYGFRLPSALDNRPLRFEEFEERSPQTIYI
                     SATPGPYEQEHSDNVAEQVVRPTGLIDPEVEIRPVKTQVDDLMSEIRQVIAQGSRILV
                     TTLTKRMAEDLTEYLSEHGIKVRYLHSDVDTVERMEIIRDLRLGEFDVLVGINLLREG
                     LDMPEVALVAILDADKEGFLRSERSLIQTIGRAARNVKGRAILYADTITGSMQRALTE
                     TERRREKQKAFNLEHGITPKGINKSVEDILEGAYIGKRKTVVAEKSPQYTHWSPQELV
                     KQINALEKQMYSHAQNMEFELAAKIRDEYLLLKEQLMKI"
     gene            3149..4327
                     /locus_tag="I9760_002266"
     CDS             3149..4327
                     /locus_tag="I9760_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAT5044027.1"
                     /translation="MDIALAKRVQKVKPSPTLAVAAKAAQMKAQGLDIIGLGTGEPDF
                     DTPQHIKLAAISAIEAGDTKYTAVDGIVELKEAVKNKFKRDNELDYQLNQILVSVGGK
                     QSCYNLCQAYLNPGDEVIIPAPYWVSYPDMVLLADGVPVIIETTPAQRYKINAQQLEQ
                     AITPKTRMIFLNSPSNPSGIAYTQNELKELGDVLKKHPQILIATDDMYEHIIWSQPFT
                     NILNACPELYDRTIVLNGVSKAYAMTGWRIGYAAGPAPLINAMKTIQSQSTSNPCSIA
                     QRAAVAALNGSNESIEEMVNAFHQRHDYVADRLQSIDGIEVIPADGTFYIFPSVQAII
                     EKRGYANDIEFSEKLLNEVGVALVPGSAFGTEGCIRISFATGIDTLKDALNRLQRFCS
                     "
     gene            4387..4462
                     /locus_tag="I9760_002267"
     tRNA            4387..4462
                     /locus_tag="I9760_002267"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4420..4422,aa:Asn,seq:gtt)
     gene            complement(4571..5518)
                     /locus_tag="I9760_002268"
     CDS             complement(4571..5518)
                     /locus_tag="I9760_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAT5044028.1"
                     /translation="MANLFSFLFLGTLGWGMSLYLMKILLVSLTPTEIVLYRMAIGAA
                     SLFILAKLFKLKTDNIRGLILDGIIVGTFNMTLPFYLTTYAVKTVSSSLASVINGLTP
                     LCTLILGMVFFSSKQKLGVLNLLSIVLGFIGIIIINSDVSIYQGSAVEFIALLATTIS
                     YGVTANYFKYHARTKEPILVSAVSAFVSAITMLIFKCATESSINSWGIPQGMNQIIAL
                     LWLGIIGSGFCLYLYCSLIQNAGAVFASMITYLMTITGVIMGMIFLHETISHQVMIGC
                     IFIFFSLILVNHAPLLEKILVSISRQISLKSARRYELNQ"
     gene            complement(5502..6092)
                     /locus_tag="I9760_002269"
     CDS             complement(5502..6092)
                     /locus_tag="I9760_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HAT5044029.1"
                     /translation="MSQSNVIIVHGAYGHPFENWFNWMKTELESLGIECFVPQLPTPD
                     GQELKNWLHVFNSTVSPMITHDTILIGHSLGSAFLLRWLEQANQSVFSTILAGSFIGT
                     VGIQKFDKINESFFENGFDWHSIINKSKQFFCYHGSNDPYISRSNFNFIANNLQARKI
                     IISQGGHLNEAAGFSSFPQLLIQLKSLLGINYGKFI"
     gene            complement(6113..7417)
                     /locus_tag="I9760_002270"
     CDS             complement(6113..7417)
                     /locus_tag="I9760_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HAT5044030.1"
                     /translation="MSIHLINYTTQPEFIKLIVQEYKNFDLTKKTILFIEPYGAVLSL
                     LRRGLEKKYNIIVLTANSDFRVVPEEFLNQAALNINIDTANNNSVLELAGTLQQHMSI
                     DAVIPGFEYFVPIASRVSALLKVPGIDCKHVLNLRRKDLMRAALNKAGLNSPRYYLVT
                     SLDDINEAVDYIGFPAICKPIDAAGSVHVKKVKNKKELLEAANRILNGNDVLWGHKLS
                     SSLLLEEYIDGKEYSLEGTVQDGVIAHFSTTEKFVSDQSEFIEIGHIVNAPIEQQLHT
                     KIQNYIDQVIKILGADNCPFHAEVRLRRDGEPVLMEIAARLAGDKIADLLCLSRGINY
                     FDYVYAAYLGIKMPLVEMKNNYAGIRFFYRPHTDMFTIVTGMDAAKLQTIEDIAIYYN
                     AHVAIPEFPKPLRRLGHVIAKSEDYNKLSKMLHDIDSDIHFN"
     gene            complement(7434..8255)
                     /locus_tag="I9760_002271"
     CDS             complement(7434..8255)
                     /locus_tag="I9760_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5044031.1"
                     /translation="MMSYMLVSNEKVIAEEWKKYAGYTVRPLPSTCAYYKDLIQSNSN
                     KDSFLIYGGTPEIRSLFQESNLKVTLTDQSELIIRAMGYLTMGAASIAANEELVVTNW
                     LNLEGIANDSCDFLIGDDAINMVEWTQFDLFLSNANRVLKSNGLFICHLLVKPQECFI
                     TNTIDDVRREYKEGVIRTKYDLASRLNFICYDTNSYAMGWQNTIEQLGINQLDQFKPS
                     FDFVGTFGRCNSKFYCPPQDAFERLLNNYFHIEEVFYPHEYEYCQFEPVYVLRKK"
     gene            complement(8261..>9028)
                     /locus_tag="I9760_002272"
     CDS             complement(8261..>9028)
                     /locus_tag="I9760_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HAT5044032.1"
                     /translation="EFKKYESSWMANEKNYFLTKYDCNVIVKDESLVRGVLQKQEVFN
                     HPIFDYLMHDASIEDLKKFAYSESIMNLEFFDYLALAVIGVSAQAKVEIMANLWDEAG
                     QGCAEKFHTVLFQNFLNDLGITYDRKQILEDLSWEGVAGINLFNYFSIYPFNKTKYFG
                     MLAATEMLDPPHYNKLIKGIMRIFSSHNIDHSYYTEHELVDAQHASGWLNHVVIPELA
                     RKPYKTQDFWLGFYMRLDSTKKYYDQLLNSLIMKKAA"
BASE COUNT         2864 a         1932 c         1575 g         2657 t
ORIGIN      
        1 atcgtcttct tctccatgga taggatagat agcagcacca acggtagtgg ccagattgat
       61 attaattcca tccaccatga gattactatt tagcaaggta agaagatgaa ttaacagctc
      121 attaatttcc aaatcctttt taagtcttgg catcaatatg gcaaattcat cactttgcaa
      181 tcttgcaacc atattaattc caagagacgc ttttagcatg tagggatcaa tcagaaccga
      241 ttttaatttt tcagcaaatt gctttacgat attattggta ttaaatgagc tgaaattata
      301 attaagatca ttaattttta tcacaataac tgctaactca ccttgtgatc ccaattgttc
      361 aatagcgagc cgtatttttt catttaacaa gcggttgtta ggcaaattgg tcaagctatc
      421 atatttgagt tttaattcca gattcccact aatattcaaa agttctttgg ttttatcaac
      481 cagtaaactc tccgctttgt taaccaatcc ataacaaaac gcttgccccc aataaacaaa
      541 cataaaaggt gttaaatcca gaatccaaat tgccgggtta gatctttgtg cctgaataaa
      601 ccctcctaag cttacagacc cagtcaattg ataggaaaca atacaacttg caatcaagat
      661 actgccaaca gaaataagca atcctaaaaa ggcataccgg ttaacatgac ctttcaggat
      721 aacagagcta tatgccaaat tagatttaat attcatggat taacagtaat tcccttatgc
      781 ctgtgaacag gctggttttt aattcctcat cccttctaca accacttcaa tgctctacaa
      841 aaaactgaat aaatggacta cttaatgatt agtatagtct aaagaacaaa taacaaatgc
      901 tatatcatat ggagtttttc taggcgtaga aaccaggtta gtgttataat ctaattaacc
      961 cgatggcgag tgattttaga accaacactc acagccaggg aagcatcctg ttatcggcgt
     1021 tgagcaagcc caccaataga gtgggaagcc tgaaccgatg cattcgctgc ccacttgaac
     1081 ctaagggttc aaaatccggt acccatcggg ttatatcttc atcaactgct cttttaatag
     1141 caggtattca tcgcgaattt tagctgccaa ttcaaattcc atgttttgag catgagaata
     1201 catttgtttt tcgagcgcat taatctgttt taccagttcc tgaggagacc aatgagtata
     1261 ttggggagat ttttcagcaa ccactgtttt tcgtttaccg atataggcac cttccaaaat
     1321 atcctcaaca gacttattaa tacccttggg agtaatgccg tgttccaaat tgaatgcttt
     1381 ttgcttttca cgtcgtctct ctgtttccgt caatgctctt tgcatcgaac cagtaatggt
     1441 atcggcatac aaaatagctc gccccttcac attacgcgct gcacggccaa tcgtttgaat
     1501 taaagaacgc tccgatcgta aaaaaccttc cttgtcagca tccaaaatcg caacaagagc
     1561 aacctcgggc atatcaagac cttcgcgcaa taaattaatc cctaccagaa catcaaactc
     1621 acccaaacgc aaatcacgaa taatttccat gcgctcaacc gtatcgacgt cggaatgcaa
     1681 atagcgcacc ttgatcccgt gctcactcag atactctgtc aaatcctctg ccatgcgttt
     1741 ggttaatgtc gtgacaagaa tacggctgcc ctgagcgatc acctgtctga tttcagacat
     1801 aagatcatcc acctgagttt ttaccggcct gatttccacc tcaggatcaa tcaatccagt
     1861 cggtcttacc acttgttcag ccacattatc cgaatgttcc tgttcataag gccctggagt
     1921 tgctgaaata taaatagtct gcggcgaacg ctcttcaaat tcttcaaaac gcaaaggcct
     1981 attatccaag gcagaaggca ggcgaaatcc ataattaacc aaggtttcct tgcgcgccct
     2041 gtctccgcga tacatcccac ctatttgagg aacagtaaca tgcgattcgt cgataattag
     2101 caaggcttcg ggcgggagat aatcaaaaag agtaggcggt gcttcccctg cttcgcggtt
     2161 ggataaataa cgcgagtaat tttcaatgcc agagcaataa cccaactcca acatcatttc
     2221 aatatcaaaa caagttcttt gctccaaacg ctgagcttct accaacttat tttgagcatt
     2281 caactcagcc agcctttcct gtaattctac ttttactttt tctactgttt ctaaaatacg
     2341 ttctctggga gtgacataat gagttttagg aaaaatagtg acacggggca accgctgtaa
     2401 aatttctccc gttaaaggat caaagcgggc aatattatca acctcatcgt caaaaagctc
     2461 aattcttatc gcttctttct cggaatcagc cgggaaaata tcaatgacat caccatgtac
     2521 ccggaattga ccgcgctcaa ggctgagatt ggttcgtgtg tactgcattt ctgcaagacg
     2581 ctttaaaatt ttacgctgat cggattgttc tccacgggag agatgtaata acatacgcag
     2641 ataggaatcc gggtcgccca atccataaat ggcagaaacc gtcgctacaa taatggcatc
     2701 tttgcgttca atcaacgctt tggttgccga caagcgcatc tgctctatat gttcattaat
     2761 ggaggcatcc ttttctataa aagtatctga agcaggcaca tacgcttcgg gctgatagta
     2821 atcgtagtag gaaacgaaat attctacggc attatcagga aagaaggctt taaactctcc
     2881 ataaagttga gccgcaagcg ttttgttcgg tgccatgatc aaagtgggtc ttttcatggc
     2941 ttgaataaca tgagctatag taaatgtttt accagaaccg gtaacgccta ataatgtttg
     3001 tttggctaaa cctgattcaa gaccatcaat caaagaggct atggctgtag gttgatcgcc
     3061 ggcaggttgg taatttgagt aaattttaaa taagtctttc atattttata ttatatgcta
     3121 gtacccctat aattcacagg agcaacagat ggatatcgca ttggcaaagc gtgtacaaaa
     3181 agtaaaacct tcacctaccc ttgccgtagc tgcaaaagca gctcagatga aagcccaggg
     3241 gcttgatatc attggccttg gaactggtga acctgatttt gacacgccgc agcatataaa
     3301 gctagccgcc atctctgcta ttgaagcagg tgataccaaa tacaccgccg ttgatgggat
     3361 agtcgaacta aaggaagcgg ttaaaaacaa attcaaaaga gacaatgaac tggattatca
     3421 actcaatcag atattggtct ccgtaggggg taaacaaagc tgctataacc tctgccaagc
     3481 ttaccttaat cctggcgatg aagtcatcat tcccgctcct tattgggtat cctatcctga
     3541 catggttttg ctggctgatg gcgttcctgt catcattgaa actactcccg cgcagcgtta
     3601 taaaatcaat gcacaacaac tggaacaagc catcactcca aaaacacgaa tgattttcct
     3661 gaacagccct tccaacccct ctggaatagc ctacacccag aacgagttaa aggaattagg
     3721 cgacgttttg aaaaaacatc cacagatact gattgccacg gatgatatgt atgaacatat
     3781 tatctggagc caacccttta cgaacatcct caatgcctgc cctgaattat atgacagaac
     3841 gatcgtatta aatggcgtgt ccaaagctta tgccatgaca ggctggcgga taggctatgc
     3901 ggcaggccct gcaccattaa tcaatgcgat gaaaaccatt caatcccaat ccacatccaa
     3961 tccatgctcc atagcccaaa gagctgccgt agccgctttg aatggcagca atgaaagcat
     4021 tgaagaaatg gtgaacgcct tccaccaaag gcacgactac gtggccgata gattgcaaag
     4081 catcgatggc atagaggtca tccccgccga cggaactttc tacatttttc ccagtgtgca
     4141 ggcgattatc gaaaaacgag gttacgccaa tgacatcgaa ttttccgaaa aattgctgaa
     4201 tgaagtaggt gtagcactgg ttccaggctc cgccttcggc acagaaggct gcatcagaat
     4261 atccttcgcc accggtattg acaccctgaa agatgccttg aaccgattac aacgtttctg
     4321 cagttaaaaa atatattaaa aacctcttga ttctctcgcg gaatcgtttt aagattccgc
     4381 ctcaattccc cggtagctca gtcggtagag cggatgactg ttaatcatta ggtcacaagt
     4441 tcgagtcttg tccggggagc caatcccagt ctgggttttg gcgaattcca agttttccct
     4501 tcctgaagct tggtaccagt tttggtaccg attttaaaat acaaaataac gaaatatatc
     4561 acgagttgat ctactgattt agttcatatc tacgagcact ttttaatgaa atttgccttg
     4621 atatagaaac caatatcttt tcaagcaaag gcgcgtggtt tactaatatc aaggagaaaa
     4681 aaatgaatat acaaccaatc atgacctgat gagaaattgt ttcgtgcaaa aatatcatcc
     4741 ccatgataac ccctgttatt gtcataaggt aggtaatcat tgacgcaaaa actgcacctg
     4801 cattttggat taacgaacag tagagatata aacaaaatcc tgatccaata attcctaacc
     4861 ataacaaagc gattatctga ttcatgcctt ggggtattcc ccaggagtta attgaggatt
     4921 ccgttgcaca tttaaaaata agcattgtaa ttgcagaaac gaatgccgaa accgctgata
     4981 caagtattgg ctctttagta cgagcatgat atttgaaata attagcagta actccgtatg
     5041 aaatggttgt tgctagaaga gctataaatt caacagctga accttgataa atactcacat
     5101 ctgaattaat aataattatt ccaataaatc caagcacgat actcaataga ttcaatacac
     5161 ctaatttttg tttcgaagaa aaaaacacca tacccaatat taaggtgcac agcggtgtaa
     5221 gcccattaat aacggatgct aatgaactgg atacagtttt tactgcatag gttgtcagat
     5281 aaaaaggcag cgtcatgtta aatgtaccaa ctattattcc gtccagaatt aacccacgaa
     5341 tattatccgt ttttaattta aatagtttag ctaagataaa aagagaagct gccccaattg
     5401 ccattcgata caaaacaatt tccgttggcg ttaaagacac caataatatt ttcattaaat
     5461 agagtgacat gccccagccc agtgttccaa gaaacaaaaa actaaataaa tttgccataa
     5521 ttaataccta ataatgattt taattgaata agtaactggg gaaatgacga aaaacccgct
     5581 gcttcgttga gatgcccgcc ttgagagatg ataatttttc tagcctgtag attgtttgca
     5641 ataaaattaa agttacttct tgatatgtaa ggatcgttgc tcccatggta gcaaaaaaac
     5701 tgtttggatt tattgattat tgaatgccaa tcaaacccat tttcaaaaaa actctcatta
     5761 attttatcga atttctgtat gccaacagtt cctataaacg aacctgctaa aatagtgcta
     5821 aatacagact gattagcttg ctcaagccac ctcaataaaa atgccgatcc caagctatgg
     5881 ccgattaaaa tcgtatcatg agtaatcatt ggtgatactg tggaattaaa tacgtgaagc
     5941 cagttcttta attcctggcc atctggcgta ggcagttgtg gaacaaaaca ttcaatacct
     6001 aaactctcta attcagtctt catccaattg aaccaatttt caaatggatg accataggca
     6061 ccatgaacaa taataacatt agattgagac attcgattct cctttatttt tatcagttaa
     6121 aatgaatgtc tgaatcaata tcatgaagca ttttagagag cttattataa tcctcacttt
     6181 tagcgatgac atgccccaag cgacgtaagg gtttaggaaa ctcaggaatt gctacatgag
     6241 cattatagta aatggcaata tcttctatag tctgcaactt cgcagcatcc atacccgtta
     6301 ctattgtaaa catatctgta tgcggacgat aaaaaaaacg aatacctgca taattatttt
     6361 tcatttctac taagggcatc tttataccca gataggccgc ataaacataa tcaaaataat
     6421 taataccacg agataaacaa agcaaatcgg caatcttatc acctgctaag cgggctgcaa
     6481 tttccataag cactggttcc ccatcacgtc gcaatcgcac ttcggcatga aatggacaat
     6541 tgtcagcgcc aaggattttt ataacctgat caatatagtt ttgaatcttt gtatgcaact
     6601 gctgttcaat gggcgcatta acaatatgcc ctatttcgat gaactcagat tgatctgaaa
     6661 caaatttttc agtagtacta aaatgagcaa taacaccatc ttgcactgtt ccttccaaac
     6721 tgtattcttt accatcgata tactcttcta atagtaaaga actagataat ttatgccccc
     6781 acagaacatc attgccattt aatatccggt tagcagcctc gagcaattct tttttatttt
     6841 taaccttttt aacgtgaacg cttcctgcag catcaatagg cttgcagata gccggaaatc
     6901 caatataatc tactgcttca ttaatatcat ccaaagaggt tactaagtaa taccgtggac
     6961 tattcaaacc tgctttattt aaagcagcac gcatgaggtc ttttcttcta agatttaata
     7021 cgtgtttaca atcaatccca ggtactttca ataaggcact gactcttgaa gcaataggaa
     7081 caaaatattc aaaacctgga atgactgcat ctatagacat atgctgctgt aatgtaccag
     7141 caagctccaa tacactgtta ttatttgcag tatctatatt gatattcagt gcagcctgat
     7201 ttaaaaactc ctcaggcacc actctaaaat cactgttggc tgttaaaaca ataatattat
     7261 actttttttc aagtcctctc cttagcaatg aaagaacagc gccataaggc tctataaaaa
     7321 gaattgtttt tttggtcaag tcaaaatttt tatattcttg tacgattaat ttaatgaatt
     7381 ctggttgtgt tgtgtagtta attaaatgaa tgctcatgtg caagttcctt atgctatttt
     7441 ttacgtaata cataaactgg ttcgaactga caatactcat actcatgtgg ataaaatact
     7501 tcctcaatat ggaaatagtt attgagtaaa cgctcaaatg catcttgggg agggcaataa
     7561 aacttgctat tacatcgtcc aaatgtacct acaaaatcaa aacttggctt gaactgatcc
     7621 aactgattga ttcctagctg ttcaatagta ttttgccaac ccatagcata ggaattagtg
     7681 tcatagcaga taaagtttaa tctactagct aagtcatact tagtcctaat cactccctct
     7741 ttatactcac gcctaacatc atctattgta ttcgttataa agcactcctg tggtttcact
     7801 aataaatggc aaatgaatag gccattactt ttaagaacac gattggcatt agataagaac
     7861 aagtcaaatt gagtccactc aaccatatta atcgcatcat caccaatgag gaagtcacat
     7921 gaatcattag caattccctc taaattaagc caattggtta caacaagttc ctcattggca
     7981 gcaatagatg ctgcacccat tgttagatag cccatggcac gaatgattaa ctcagattga
     8041 tccgttaagg tgacctttaa atttgattcc tgaaagagtg atctgatttc tggtgttcca
     8101 ccataaatta aaaatgagtc cttattagag ttagattgaa ttaaatcttt ataataggca
     8161 caggtactag gtaaaggccg cacagtgtat cctgcatatt ttttccattc ttctgcaatt
     8221 accttttcat ttgagacaag catgtaagac atcatccacc tcacgcagct tttttcatga
     8281 tcaagctatt taataattgg tcataatatt ttttggtcga atccagtcgc atgtaaaaac
     8341 ccaaccaaaa atcctgcgtt ttataaggct tacgtgctag ctctggtatg acgacatggt
     8401 taagccaccc actcgcatgc tgagcgtcca ctagttcgtg ttcggtgtaa taggagtgat
     8461 cgatattatg cgaactaaat attctcataa tgccttttat taatttgtta taatggggag
     8521 ggtcaagcat ctctgttgcc gcaagcatgc caaaatactt agttttatta aaaggataga
     8581 tagaaaagta attgaataaa ttaattccag ccactccttc ccaggacagg tcttctaaaa
     8641 tttgttttcg atcataggtt atacccaaat cgtttaaaaa attttgaaac aatacagtat
     8701 gaaatttctc agcacatcct tggcctgcct catcccaaag gtttgccata atttctacct
     8761 ttgcttgagc agaaacccct atcactgcta atgctaaata gtcaaagaat tccaaattca
     8821 taattgattc gctataggcg aattttttga ggtcctcaat gctggcatca tgcattaaat
     8881 aatcaaagat aggatgatta aatacctctt gtttttgcaa cacgcctcta acaagagact
     8941 catcttttac aattacattg caatcatatt ttgttaaaaa atagttcttt tcatttgcca
     9001 tccatgaaga ttcatatttt ttaaactc
//
LOCUS       DACTJE010000130         9028 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.130, whole genome shotgun sequence.
ACCESSION   DACTJE010000130 DACTJE010000000
VERSION     DACTJE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.130"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            232..666
                     /locus_tag="I9760_002273"
     CDS             232..666
                     /locus_tag="I9760_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HAT5044033.1"
                     /translation="MKLWKVLCSFVIAVCYLPLAFAASQSPAGTWTTIDDKTGAKRAV
                     VNLKVSNGTLSGTIVKVYPQPGDTGICSKCPGAFKDKKIQGLTFVWGLKDEGNGVWSG
                     GSILDPKTGKIYKAKMTLQGNKLYVRGYFGVSALGRTQTWIR"
     gene            844..2226
                     /gene="dnaB"
                     /locus_tag="I9760_002274"
     CDS             844..2226
                     /gene="dnaB"
                     /locus_tag="I9760_002274"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAT5044034.1"
                     /translation="MLDLQISKKTVDPLKRPPHSVEAEQSIIGGLMLDNQVWDKVSNK
                     LCETDFYRTEHRILFRAISALAKKDQPFDVVTLLDALKTRNELDDAGGEAYLFELANN
                     TPSVANVTAYADIVREKSVQRQLIAVATEIADSAYNPAGREVPELLDMAETKVFAIGE
                     QTASDGGPENIKSILVRAVEKIDALYHNGDAITGLATGLSDLDEMTSGLQPSDLIIVA
                     GRPSMGKTTLVMNMAEHAAIKAGKPVLVFSMEMPADSLAMRMMSSLGRIDQHRIRTGK
                     LDDNDWPRVTSAVHMLSEAPLFIDDTPALSPGEMRARARRLAKEHGSLGLIVVDYLQL
                     MKVPGFNADNRTAEISEISRSLKSLAKELQVPVIALSQLNRSLEQRADKRPVMSDLRE
                     SGAIEQDADLICFIYRDEVYNEDSPDKGTAEIIVAKQRNGPIGKVRVAFLGKYTRFED
                     LAFNGYQGVE"
     gene            2228..3301
                     /gene="alr"
                     /locus_tag="I9760_002275"
     CDS             2228..3301
                     /gene="alr"
                     /locus_tag="I9760_002275"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAT5044035.1"
                     /translation="MSRPTRLVIEPSALLHNLSQIKHLAPGKKVIAMVKANAYGCGVR
                     EVAPVLDGRIEAFGVACLEEALAIRALGVETPCILFQGVFSSDELSVAVENDFACVLH
                     HAQQLEWLIKTPLPYPIKVWVKVNTGMHRLGFKIHELQKVMGALQTCTWVDKSIGLMT
                     HLACADEPHRPENQQQISLFQEISIPGFRQRSIANSAAIISFPDSQADVVRPGIMLYG
                     VSPFANQNAHDLGLIPVMRFMSAISAIHDNPSFAQVGYGGTWKSDKPSRIGVVAAGYG
                     DGYPRHISEKTPVWVRGREVSIVGRVSMDMLTIDLTEHPDIEIGDEVELWGTHVLVER
                     IAKSAGTVGYELLCQISERVRYK"
     gene            3374..3823
                     /locus_tag="I9760_002276"
     CDS             3374..3823
                     /locus_tag="I9760_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAT5044036.1"
                     /translation="MKKLIVASLSLTFMLGGCASMNKEGVGTITGGVVGGLLGSQFGG
                     GSGKVAAAGAGALLGAYLGGQIGKTMDKVDRLEMQRALETAPTGKAVAWSNPDTGYRY
                     TVQPTRTYYNAQQPCREYITKAMIGGKTQQIYGKACRQADGSWRVVN"
     gene            4131..4565
                     /locus_tag="I9760_002277"
     CDS             4131..4565
                     /locus_tag="I9760_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAT5044037.1"
                     /translation="MAHLIHSVLPVPPRKIAYIHPDDSVKECIKQMVEKDIGALVVFD
                     NDAHLIGIVSERDILRCYFHKSLSLETAKVSDVVYTNVTILSPHDSVEKAMQVITETK
                     RRHVLIQEEGELLAILSIGDLLYHVLEDKLRVIEHLENYIHS"
     gene            4674..5861
                     /locus_tag="I9760_002278"
     CDS             4674..5861
                     /locus_tag="I9760_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044038.1"
                     /translation="MKTMKQMIRLTMLLLMLSFNLWAINSSSPWYTEEKGKGLVINVE
                     LYLSSTCPHCHKADEFFKEIEKHYTWLHVERYIIDKDKKALKRFGDLLTELNRYDFAV
                     PSIFFCNSRWVGFATAQTTGKDLLNGIEYCKEEIVKNGRLTPATENVLNRWGNASLFD
                     SNITGSPSTNTFIFVVAIIDAISPCALFTITAFLGLLFMIEKRKLQFISGSVFILTVA
                     GVHYFQQVYPTLFFESLSWFRIPAALVGLFAFYFAGQYYKKHSIQPLFIVLAFLLALT
                     VPMFQQTCLMNWSYVFEQWLHNQNVSGVQMGLYQFAYQLIYIFPLIILMFLYWILMKI
                     HFFKKFKTKLTTIGFLYILAIALLLIVYPYALSNLALSLFLIVSLGISGLLLNWFNAS
                     KMT"
     gene            5973..6269
                     /locus_tag="I9760_002279"
     CDS             5973..6269
                     /locus_tag="I9760_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HAT5044039.1"
                     /translation="MDDNNSEFKHLFPIAVEVTEGKTYVWCGCGKSKTQPFCDKENCG
                     DKAVSFIAELTEDVYFCNCKQTKNPPFCDGSHAKILLEVVKKKAKQMNRKGSLK"
     gene            6266..7816
                     /locus_tag="I9760_002280"
     CDS             6266..7816
                     /locus_tag="I9760_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT5044040.1"
                     /translation="MIDQELKKKIVNTISQYEHKCDKMQSQINTLRSVINQLIINPIG
                     INYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEILSKMQRKKAENSRLVNGLIKQGV
                     ESIGRIANKSQDKRAVTKLQKMLDTEMESHAILIRFNQVLTQCISSVIKEMEKLPQIT
                     KVDTPQKSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGVIKTNLEHALTDEELSTII
                     DNLTELVVESFNVEQNRFKGFLQQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQ
                     DNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIREEEYAKQVSELQAR
                     LQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYDEHILDSFQRWKRSSSALSLAV
                     CDIDHFKRINDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKE
                     AFGILEKLRKLVEDCLFYYRDKKVDVTVSFGLTTVTEEDDIESLFMRADNAMYKAKNA
                     GKNRVEIL"
     gene            7968..8957
                     /gene="lipA"
                     /locus_tag="I9760_002281"
     CDS             7968..8957
                     /gene="lipA"
                     /locus_tag="I9760_002281"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAT5044041.1"
                     /translation="MGKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPK
                     WLRIVNHTTPAYSQVKEQVQKHRLATVCEEAKCPNISECWSHGTATIMLMGAVCTRAC
                     RFCSVDTGNPHGWLDAEEPENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRA
                     IKKRSPRTKVEALTPDFQGSERDVAVLLDSGVDVFAQNVETVERLTHPVRDNRAGYQQ
                     TLNVLAFAKKYRPDVLTKTSLMLGLGETDEEIIQTMDDLRTHHVDILTLGQYLQPTKN
                     HLPIARYVTPETFSELRQIGLKKGFFEVASGPLVRSSYRADRVFKRDNLGLDV"
BASE COUNT         2790 a         1513 c         1962 g         2763 t
ORIGIN      
        1 cctgacggtc gcggctcgga ttatggatca aaccaatcct caaccatcaa accgagctgc
       61 gaccgtcagg gagcggataa taaccctata tttattttaa agatttcagt agcaaagtta
      121 cctatcatgg cttatgcttt attacaagct cttgtataac ttgaactttg tattccaaaa
      181 gagtacagaa tttatacaag aggtatactg attcaattac ggagattact tatgaaatta
      241 tggaaagtgt tatgtagttt tgtaattgct gtatgttatt taccattagc ctttgcagca
      301 tcacagtctc ctgccggtac ctggacaacc attgatgata aaaccggagc taagagagca
      361 gtagttaacc ttaaagtatc aaatggtact ttaagtggta ctattgtgaa ggtatatcca
      421 caaccaggtg ataccggaat ttgttctaag tgccctggcg cattcaagga caaaaaaatt
      481 caagggttga cttttgtttg gggattaaaa gatgaaggca acggcgtctg gagtggtggc
      541 tctatcctcg atcccaaaac aggaaaaatt tataaggcta agatgacact gcagggtaat
      601 aaactgtatg tcagagggta ttttggagtt tctgctctag gacgtactca aacctggatt
      661 cgataagtag tactgaagga tgtttttata tataatgtgg gatagtgctt tctcttgtca
      721 ttgaaagcag ttggtactat cccgcatttt gtttttagtg ttatgatcat ttgcaattag
      781 attaggaatg tctttaaata aattgtcttc cctataaata aattctcaaa aaatattatt
      841 accatgcttg atttgcaaat atctaaaaaa acggttgatc cactaaaacg ccccccgcat
      901 tctgtagagg ccgagcaatc cattattggt ggtttgatgc tcgacaatca agtctgggat
      961 aaggtaagca ataaattatg cgaaaccgat ttttatcgta ctgagcaccg aattcttttt
     1021 agagccattt ctgctttagc caaaaaagat caaccttttg atgtagtgac gcttcttgat
     1081 gctttaaaaa cacgcaacga gttagatgat gctggtggag aagcctattt atttgagttg
     1141 gctaataaca caccaagtgt agcgaatgtg accgcttatg cggatattgt tcgggaaaaa
     1201 tcagtccaga ggcaactgat agcggtagca acagaaattg ctgattccgc ttataatcct
     1261 gctggaagag aagtgcctga gctattggat atggccgaaa ccaaagtatt tgctattggt
     1321 gaacaaacag cgagtgacgg gggtccagaa aatattaaat ccattttggt acgggcggta
     1381 gagaaaatag atgccctcta tcataatggt gatgcgatta caggccttgc gacaggctta
     1441 tccgatttgg atgaaatgac atcgggttta cagccttctg acctgattat cgttgctggc
     1501 cgtccatcga tgggaaaaac aactttggtg atgaatatgg cagagcatgc ggcaattaaa
     1561 gccggtaagc cagtattggt tttctctatg gaaatgcctg ctgattccct ggcaatgaga
     1621 atgatgtctt ccttggggcg aatagatcag catcggattc gtactggtaa attagatgat
     1681 aatgactggc cacgagtgac ctccgcggtg catatgcttt ctgaagctcc tttgtttata
     1741 gatgatactc cagctttaag tcctggagaa atgagggcac gtgccagacg tttagcaaag
     1801 gaacatggtt cgttagggct tatcgttgtc gattatttac aattaatgaa agtgcccggg
     1861 tttaatgcag acaatagaac agcggaaatc tccgaaattt ctcggagtct taaatcgctg
     1921 gcaaaagagt tacaggttcc cgtaattgct ttatctcagc tgaatagaag cctcgagcag
     1981 cgagcagata agcggccagt gatgtcagat ttgcgagaat caggagcaat tgagcaggat
     2041 gctgacttaa tctgttttat ttatagagat gaagtatata atgaggacag tcctgataaa
     2101 ggcactgcgg aaatcattgt tgccaagcaa agaaatggac ccataggcaa agtcagggta
     2161 gcctttttgg gtaagtacac acgatttgaa gatttggcat ttaatggtta tcaaggggta
     2221 gaatagggtg tcaagaccta cacgtttagt gatagagccc agtgctttat tgcataattt
     2281 atctcaaatc aagcatttag ctccaggcaa aaaagttatc gctatggtaa aagccaacgc
     2341 ctatggctgt ggagtcaggg aagttgcccc tgtccttgat gggcgtattg aggcttttgg
     2401 agtagcctgc cttgaggagg ctctggctat tcgagcttta ggtgttgaaa ccccgtgcat
     2461 tctctttcag ggcgtcttta gttctgatga attgtcagta gccgttgaga atgatttcgc
     2521 ctgtgttctt catcacgcac agcagttgga atggctaatc aagactccgt tgccttaccc
     2581 gataaaagta tgggtgaagg taaacacagg catgcacaga ctgggattta aaatccatga
     2641 attgcaaaaa gtgatggggg cattgcaaac ctgtacctgg gttgataaaa gtatagggtt
     2701 aatgactcat ttggcttgcg cagatgaacc tcatcgtcct gagaatcaac aacagatctc
     2761 tttgtttcag gagatatcca tacctgggtt tcgccaacgc agtatcgcta attcggcggc
     2821 aattatttca ttccctgatt cgcaggctga tgtggtgagg cccgggatta tgttgtatgg
     2881 tgtttcccca tttgcaaatc aaaatgccca tgatttaggt ttaatccctg tcatgcgttt
     2941 tatgtcagcc atcagcgcga ttcatgataa cccatctttt gcccaggtag gctatggtgg
     3001 aacctggaaa agtgataaac cttcgcgaat tggtgttgtt gcagcaggtt atggcgatgg
     3061 gtacccacgg catatttccg agaaaacacc ggtttgggtt agagggaggg aggtgtctat
     3121 tgttggcaga gtttctatgg atatgctgac catcgatttg acagagcacc ctgatattga
     3181 gatcggcgat gaagttgagc tttggggaac acatgtttta gtagagcgca ttgccaaatc
     3241 agcaggtaca gtaggatacg aattattatg tcaaatttcg gagagagtac gttacaaata
     3301 agttatggat aataactgtt cgtggtggta gaatgacaac cgattttgta actctttaaa
     3361 caggtgaact gtaatgaaaa aattaattgt tgcatcatta tccctaacat tcatgctagg
     3421 tggctgtgcc tcaatgaaca aagaaggtgt aggtacaatt actggtggtg tggtaggtgg
     3481 attacttggt agccagtttg gtggtggttc aggtaaggtt gcagctgcag gagcaggagc
     3541 attattagga gcttacctgg gtggccaaat tggaaaaaca atggataaag tagaccgact
     3601 tgaaatgcaa agggccctcg aaacagctcc aacaggtaaa gcagttgcat ggtcaaatcc
     3661 ggatactgga taccgttata ctgtccagcc aacgagaact tattataatg ctcaacaacc
     3721 ttgccgtgag tacattacca aagccatgat tggtggaaaa acccaacaaa tttatggtaa
     3781 ggcctgtcgt caagctgatg gttcgtggcg tgttgtgaat taattaattg attaagattg
     3841 ttggggaaaa cccaacaatc tcattttatt attctaacca attaagacag aacttacgaa
     3901 aaatccccgt ctctttgtta aaaaatgttt ttaattcggt catttggctt atctaaattc
     3961 cttcgttaaa atcatttttt gccttgacct tggagttttc aatatacaaa tcctacgata
     4021 atcttttata aaaatctttc ctgctttcga ttaatgagct taacccctcc ttgttttgtg
     4081 ctatatttat acagcgagtt gctaatttag tattaatcag ggagagtgca atggctcact
     4141 taattcatag cgtactgccg gtgccaccaa ggaaaatcgc ttatattcat cctgatgata
     4201 gtgtcaaaga atgtattaaa cagatggttg aaaaagatat aggtgcatta gttgttttcg
     4261 acaatgatgc ccacttgatt ggtattgtga gtgaaagaga catacttcgc tgttattttc
     4321 ataagagtct aagtttggaa actgcaaaag tatctgatgt tgtttatacc aacgtcacga
     4381 tcttaagtcc acatgattcg gtagagaaag ccatgcaagt gataactgaa acaaaaagaa
     4441 gacatgtatt aatccaggaa gaaggtgagc tattagctat attgtcaata ggagacttgt
     4501 tgtaccacgt actggaagat aaattgcgag tgattgagca tttggaaaat tatattcact
     4561 cctgaaatat aaactcttat ttcagattta tattttttgc tttaaacgtg agtgtctggc
     4621 tatttcatac aatcgatcct aagtgctata gtattcattt acaaagaaat ataatgaaaa
     4681 ccatgaagca aatgattcga ttgacaatgt tacttctgat gctcagtttt aatttatggg
     4741 caataaattc ttcatctccc tggtatacag aagaaaaagg taaagggctt gtaattaacg
     4801 ttgaattgta tctttcatct acttgtcctc attgtcataa ggccgatgaa tttttcaaag
     4861 aaattgaaaa acattatacc tggttgcatg ttgagcgtta tattattgat aaagataaaa
     4921 aggctttgaa acgctttggc gatttattga cagaacttaa tcgatatgat tttgccgttc
     4981 catccatatt tttttgtaat tcccgttggg ttgggtttgc tactgcacag acaaccggaa
     5041 aggatctgtt aaacggcata gaatattgta aagaagaaat tgtgaaaaat ggaagattga
     5101 ctcctgcgac cgagaatgtt ttaaacagat gggggaatgc gagtttgttt gattccaata
     5161 tcacaggaag cccttccaca aatacattta tttttgttgt agccataatc gatgcaatca
     5221 gtccatgtgc tttatttacg attactgcat ttttaggtct tttattcatg attgaaaagc
     5281 gaaagctgca atttattagc ggttccgtgt ttattttaac tgttgcaggt gttcattatt
     5341 ttcaacaggt ttatcctacc ctgttttttg agtcgctttc atggttccgt attccggctg
     5401 cattggtagg cctttttgct ttttatttcg ctggccaata ttataaaaag cattctatcc
     5461 aaccattatt tattgttttg gcttttttat tggctcttac agtgccaatg tttcaacaaa
     5521 cctgtttgat gaattggtct tatgtgtttg aacaatggtt acataaccaa aatgtatcag
     5581 gagttcagat ggggctatat caatttgctt atcagcttat ctatatcttt cctttaatta
     5641 tcctgatgtt tctctattgg attttaatga aaattcattt tttcaaaaaa tttaagacaa
     5701 aattaactac tataggtttt ttatatattt tagctatcgc actcttatta atcgtttatc
     5761 cctatgcctt gtctaatttg gctttatcat tatttttaat tgtctcattg gggatctccg
     5821 gtctgttact gaattggttt aatgcttcta aaatgacatg agggttcaat cggcataaaa
     5881 taattataaa ttctgaatat ctttcatttt atggcataca attaaaaatg agccaggctt
     5941 agaagctgat cgcttatgga gaaattctca tcatggatga taataattca gagtttaagc
     6001 atttatttcc aatagctgtg gaggtaacag aaggaaagac ttacgtatgg tgcggttgtg
     6061 gcaaaagcaa gacccagcct ttctgtgata aagagaattg cggggataaa gcagtctctt
     6121 ttatagcaga gttgactgaa gacgtttatt tttgcaattg caaacaaacc aaaaaccctc
     6181 ctttttgtga tggatcgcat gcaaagattt tgcttgaagt ggttaaaaaa aaggcaaaac
     6241 aaatgaacag gaaaggaagt cttaaatgat tgatcaagaa ttaaagaaaa aaattgttaa
     6301 cacaattagc caatatgagc acaaatgtga caagatgcaa agtcaaatta atacgctcag
     6361 aagtgtgatt aatcaattga ttattaatcc gattggaatt aattatgagt tagatgatcg
     6421 cctttcgcaa ttaagaaatt atttagatac tgaatttgaa cctgctgtca tagaaaaaaa
     6481 agtgaaacgt ttggcagaaa tactttccaa aatgcaaagg aaaaaagcgg aaaattctcg
     6541 tttagtcaat ggactaatca aacagggagt ggaatccatt ggtcgtattg ccaataaatc
     6601 tcaggataag agggcagtta caaaattaca aaaaatgctg gataccgaga tggaaagcca
     6661 tgcgattttg attcgtttta atcaagtctt aactcaatgt atttcttctg tgattaagga
     6721 gatggagaag ttaccacaaa ttacaaaagt cgataccccc caaaaaagtt atcagtctga
     6781 gattagttta aaagtaaatg acagcttgca acaattactt aaccatttat ctatccctca
     6841 ggacttggat tccaagaggg gagtcataaa aacaaatttg gaacatgcac tcactgatga
     6901 agagcttagc actattattg acaatttgac ggaattagtc gttgagtcat ttaatgttga
     6961 gcagaatcga ttcaaagggt ttcttcaaca gttaaccgat cagctcaatg attttgaaat
     7021 ctatttaaga gtatcaggag atgctcgcaa aaaggcaagt gaagacagtc gtttattaga
     7081 gtgtgggatt caggataatc ttgatcaaat caagaatcat ttggatacct ctaaaacgat
     7141 tgaggaatta tcctcaaagg tgagccaaaa tataaaaatg attggtgaca ggttaaaaga
     7201 atatcgacac aatgaaaaaa ttcgagagga agaatatgca aagcaagtat ccgagttgca
     7261 agctagatta caggaatcag agcaaaatgc ggaagaaata aaaaatttgc ttgcttttca
     7321 gaaatacaga attaaccacg acagcttaac aggcttgcca aatcgggaat cttatgatga
     7381 gcatatcctg gattcattcc agcgctggaa acgaagttca agcgcattgt ctcttgctgt
     7441 gtgtgacatc gatcatttta aacgtattaa tgataatttt ggccatcttg caggagataa
     7501 ggttcttaaa aaggtagcta tgatttttaa atcatcaatc agaacggttg attttatcgc
     7561 tcgaataggt ggtgaggaat ttgtgtttat ttttgaacaa accagcagca aggaagcttt
     7621 tggaattttg gagaaattac gtaaattagt tgaagattgt ttgttttatt atcgagacaa
     7681 aaaagtcgat gttactgttt cttttgggct aactacggta accgaagaag atgatattga
     7741 atctttattt atgcgggcag acaatgccat gtataaagct aaaaatgcag gaaagaacag
     7801 ggtagaaata ttatagtgga atatatttcc aacaattttt aattatacta ttttcggagt
     7861 aaatccatgg taacagatga ttttcctctg ataacgattt ttaaatgaac ttgatcttat
     7921 ctttgtgatt tgggatcatc ataactcttt gtatttcgga tggcgtcatg ggcaaattga
     7981 tcgatattcc aatcgttgtg gaaagtggac aaaaatataa gacatctcag ggtgtgacag
     8041 ctattaaaga tggaataaaa tcatcaggtc aagatcatga gcgattgcca aaaccgaagt
     8101 ggcttcgtat agtcaatcat acaactccag cctatagcca agttaaagag caagtgcaaa
     8161 aacatcgttt ggcaacagtt tgtgaagaag cgaaatgccc caacatctca gaatgttggt
     8221 ctcatggaac tgcaactatt atgttaatgg gagcagtatg tacccgggct tgccgatttt
     8281 gttctgtgga tacaggaaat ccgcatggtt ggcttgatgc agaagaacct gagaataccg
     8341 cagaaactgt ggccttaatg aatctggatt atgtggtttt aacctcagtt aaccgtgatg
     8401 atttacctga tgggggggcg aatcattatg ccaaaaccat tagagcaatc aaaaaacgat
     8461 ctccccgaac caaagtcgaa gcgctaaccc ccgattttca aggaagtgaa agagatgttg
     8521 ctgtattgtt agatagtggc gttgatgtgt ttgcgcaaaa tgtagaaacg gttgagcgcc
     8581 tgacacaccc ggtaagagat aatcgagcgg gttatcagca aaccttgaat gtactggctt
     8641 ttgctaagaa atatcgaccg gatgttctga ccaaaaccag tttgatgttg ggtttgggtg
     8701 aaaccgatga ggaaattata cagactatgg atgaccttcg aactcatcac gtggatattt
     8761 tgactttggg acaatatttg caaccaacta aaaatcattt acctatagca cgttacgtaa
     8821 ctcctgaaac tttttctgaa ttaaggcaaa ttggtctaaa aaagggcttt tttgaagtgg
     8881 cttcaggccc attagttcga tcaagctata gggctgaccg agtgttcaaa cgggataatt
     8941 tagggcttga tgtttgaagt tagtgaacag cttgtgtttt tcgaattggc tataaataat
     9001 tttattatta gttaaagaaa ttagattt
//
LOCUS       DACTJE010000131         8946 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.131, whole genome shotgun sequence.
ACCESSION   DACTJE010000131 DACTJE010000000
VERSION     DACTJE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8946
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.131"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            132..1658
                     /locus_tag="I9760_002282"
     CDS             132..1658
                     /locus_tag="I9760_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5044042.1"
                     /translation="MLILSKRRGLWSSMPQGTFALFFIQVVSTLSFSVLYSTLVLYMK
                     GKLGLPIATANSIMGVFIAYNFALHLLGGFWGGRLLSNRSLFCVGMIAQIVGCVLLSI
                     GNTTFLYYGLAAFLTGSGLNVTCVNCMLTQQFAPEDSRRETAFLWNYAGMNIGFFIGF
                     SLSGAFQISQNYQRLFLLSSLGNLVALLICLYFWQQLHDKNTTYSRKDKPAQRKATAF
                     GIGLIFVLPFILRQLLQYADWANKLVIVTGIIMLILILILAQQQSTKEARDKIYGFAV
                     LMLVGTVFWMLYQIGPMGLTVFIDHNVQRQYGNWIIPPQWFQNINTVVIVFGGPLLGF
                     ILNRMRQNGSQINIPTQFAFALFFIGVAFAILPIGINYANQHGMVNPGWVVISFILQS
                     IGELLISPIGYAMIGALAPTSLQGVMMGMWMLNTGVGATLSSYSSNLMIIGQESTSPL
                     LTNEGYSHVFLMLGLFAIGSSVILFILVPKLRKLIKEKNLNGFKQSGLEVTERVALCE
                     "
     gene            1651..3651
                     /gene="mnmC"
                     /locus_tag="I9760_002283"
     CDS             1651..3651
                     /gene="mnmC"
                     /locus_tag="I9760_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAT5044043.1"
                     /translation="MSSPFVPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFV
                     EGNNLINRWQQLPTNESNVFTIAETGFGTGMNFLLTWKLWEKFAPQNARLHYISCDKH
                     PLKKNDLIKCLQKWPELSVQAEKLIAHYPVLTPGYHHLTFSNNQITLTLMLGDVLECY
                     EQLLFCGDINLEHQLRESYVNAWYLDGFSPSKNQSMWSDNLFTVIAMLSKESTTVATY
                     SASSIVKTALTNAGFVIEKRKGFGPKRHMICAHYEKAYSSSKKNRHTPWHINYPVTKD
                     ERTALIVGGGLAGCFIANSLAKRGWEVTILEEKEKVGCGGSANQQAVLFPKLSTYKSP
                     FTQFMLYSFLYANDVYKELLKYYDLGELKGSLLLAHNEKEKANQQSLIHWLELYPELG
                     QLVDEKQSSELSGISLPCGGLFIPSSGWINSPELCDILIDNKRISLITGNRVQSINYN
                     QKNWVVNGIEASVLILANGQQVNYFHETNHLPVKAIRGQMTTIQSTQESTKLKIPLCA
                     EGHVLPALNNSHKVGASYDIGTSEPELNALDDQLNLARLKRIAPDIMWSQNVLDHWAG
                     IRAASPDYLPIVGPLPNALEFKEIYSELKSNSKRWIAEAAPCYPNLYVCAAFGSRGLT
                     TIPLATEWLAGLINKEISILPRKLIQAISPARFLRKKIIQGP"
     gene            3737..4522
                     /locus_tag="I9760_002284"
     CDS             3737..4522
                     /locus_tag="I9760_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAT5044044.1"
                     /translation="MQNIEFYQIDAFTDKVFHGNPAAVCILDDWLNDELLKAIAAQNN
                     LAETAFIIKDDQDFHIRWFTPNGEINLSGHATLAAGFALLESNKGPSNSIVFHHSSGQ
                     ISVEKRKEKFVLNFPRIGYKKTLSPDPIVSLLDQPAIEVFETDLDYLVLLEHESQIRN
                     LQIDLFALAKLPKRGIIVTSTTTEADFYSRCFYPKHSIAEDPVTGSAHCILAPFWAEK
                     LNKNSLYAIQGLHRKGELYCELERNRVLISGHCRWYLRGHLVI"
     gene            4728..5846
                     /gene="prpC"
                     /locus_tag="I9760_002285"
     CDS             4728..5846
                     /gene="prpC"
                     /locus_tag="I9760_002285"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HAT5044045.1"
                     /translation="MSSKSGGLAGVVAGQSAIATVGLAGKGLNYRGYSINDLAEYASF
                     EEVAYLLHYGELPTQKELDVYIKKLVNLRHIPDVLKTVLKLIPKNTHPMDVLRTACSF
                     LGTIEPEENFKQQYEIADRLLALFPGIMCYWYTYHFKNKEISGLSEEDTTGGHFLALL
                     HGRKPSKIETDMMNVSLILYAEHEFNASTFAARVTAATLADFYSAITSAIGTLRGPLH
                     GGANEAAMGLIERFKTPDEAAIGIKQMLASKELIMGFGHRVYTTCDPRSDIIKKWSFR
                     LGEEKGNLLLYHISERIEEIMWQEKKLFPNLDFYSASAYHYCDIPTHLFTPIFVMSRI
                     TGWSAHVFEQRANNKLIRPTSEYIGPDSRPFPAIETRG"
     gene            5848..7296
                     /locus_tag="I9760_002286"
     CDS             5848..7296
                     /locus_tag="I9760_002286"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HAT5044046.1"
                     /translation="MHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMD
                     TLGCGILALNFQECTKLMGPIVPGATLPGGARVPGTNYELDPVQAAFNIGTMIRWLDF
                     NDTWLAAEWGHPSDNLGAILAVADYLCRENCNHGRPPILMHDVLTAMIKAHEIQGCLA
                     LENSFNRVGLDHVILVKVASAAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNA
                     GSRKSWAAGDATSRAVRLALIAKAGEMGYPSALTAQTWGFYDVLFGKKQFKFQRPYGS
                     YVMENVLFKLSYPAEFHAQTAVECAVKLHPIVKNRLEDIARIELITHESAIRIISKQG
                     ALYNPADRDHCLQYMVAVGLLFGDLKAEHYEDDVAADPRIDALRAKMHVTENERFSRD
                     YHDPEKRSIANSMRIIFKDSSESELITIEYPIGHKRRREEGIPVLMSKFKRNLSTRFS
                     TDMVEKIIYAMSDTNTLSSMKVSDFMAMWIVN"
     gene            complement(7421..8236)
                     /locus_tag="I9760_002287"
     CDS             complement(7421..8236)
                     /locus_tag="I9760_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAT5044047.1"
                     /translation="MKRYVFMLSDGTGITAETLGNSLITQFENIQFEKITIPYIDSTH
                     RAESVVLRINQCFSEQGTKPLVFMTLVDPEIRQAIKKAHACVFDLFSIFIGPLENELE
                     EKSSYTVGRTHGVANVKSYSHRIEAIDFALSHDDGIKTRGYDKADIILIGVSRCGKTP
                     SCLYMALQYGILAANYPFTEEDLVGFRLPEVLRPYKQKLFGLTIDAQRLQQIRSERRP
                     NSKYASAEQCRLEVTEVEAMYQRENIPYINSTKYSIEEISTKVLAIAGLQRKI"
     gene            8370..8825
                     /locus_tag="I9760_002288"
     CDS             8370..8825
                     /locus_tag="I9760_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044048.1"
                     /translation="MQHNIYENSKLHILGIICLVTSLGLFFFSLYIVPFLIWQLDYDI
                     PDFISNMIAYGEDEYYFSPITSKTLVWLIFFTPSIIMGYLSYYISNYIDKNYLEPQEK
                     PLESMQEKSPSKPAGRGRDSTILGLKIILLMVLIVAIIFLLQILFDLTA"
BASE COUNT         2832 a         1586 c         1746 g         2782 t
ORIGIN      
        1 attattaaaa tatatttaat tcattttaga tactttttca cttgctgacg aaaggtggtt
       61 tttctctgat ttgtccacaa gggttagtgt taatattcaa gaagagctca actagcaagg
      121 gaaagcatat aatgttgatt ttgagtaaaa gaagaggact atggagttcc atgccccaag
      181 gtacatttgc attatttttt atacaagttg tttccacttt aagctttagt gttctttatt
      241 caactcttgt attgtacatg aaaggtaaac ttggtttacc catagccaca gcaaacagca
      301 tcatgggggt atttatagcc tacaattttg cgctgcatct cttaggtggt ttttggggtg
      361 gcagactgct ctctaatcgc tccttatttt gtgttgggat gatcgcgcaa attgtaggat
      421 gtgttttgct ctctatagga aatacaactt ttttatatta tggcttggct gcattcttaa
      481 caggttcagg gttaaatgta acatgtgtaa attgtatgct gactcagcaa tttgcacctg
      541 aagactcccg aagagaaacc gcatttctct ggaattatgc gggaatgaat attggctttt
      601 ttattggatt tagcctcagt ggggcgttcc agatttctca aaactatcaa aggctttttc
      661 ttttaagcag tttaggtaat cttgttgctt tgcttatatg cctttatttc tggcaacaat
      721 tacatgataa aaacactact tactcaagaa aggacaaacc tgcacaaaga aaagcaacag
      781 catttgggat tgggctaatt tttgtacttc catttatttt aagacaatta ttgcagtatg
      841 ctgattgggc caataaattg gttattgtca ctggaattat tatgctcata ttaatcttga
      901 tattagccca acaacaatct acaaaagaag caagagacaa gatttatgga tttgcagtat
      961 taatgttggt tggtactgta ttttggatgt tatatcaaat tggcccaatg gggttaaccg
     1021 tattcattga ccataatgtg caacgacaat atggtaattg gattattccg ccacagtggt
     1081 ttcaaaatat taatactgta gtcatagtat tcggagggcc actattgggt ttcatcttaa
     1141 atagaatgcg tcaaaatgga tctcagataa atattcctac tcaatttgct ttcgcattat
     1201 tttttatagg ggttgctttt gctatcttac caataggtat caattatgct aatcagcatg
     1261 gtatggttaa tccgggttgg gtagtaataa gctttatatt acaaagtatt ggcgaacttc
     1321 taatatctcc aattggctat gcgatgatag gagcgttggc cccaacctca ctccagggtg
     1381 tcatgatggg aatgtggatg ctgaatactg gagttggtgc tactctatcc agttatagct
     1441 caaacttaat gattattggt caagaaagta cttctccttt attaaccaat gagggttaca
     1501 gtcatgtctt tttgatgctt ggtttgtttg caataggatc atccgttatc cttttcattt
     1561 tagtgcctaa actaagaaaa ctgatcaaag aaaaaaactt gaatggcttt aaacagtctg
     1621 gcttagaggt aactgaaaga gtagctttat gtgagtagtc cctttgtccc aattataacg
     1681 gcagatattg attggcgtga tgatctgccg tattccttac aatttgatga tatttactat
     1741 tctgctgaag gtggaattaa tcaaagtctt tatgtttttg tcgaaggtaa taatttgatt
     1801 aatcgttggc aacaattacc aaccaatgaa tcaaatgtat ttacaatcgc tgaaactggt
     1861 ttcggtacag gtatgaattt cttattaacc tggaaactat gggaaaaatt tgcacctcaa
     1921 aatgcccgct tacattatat ttcctgtgat aaacatccat taaaaaaaaa tgacctaatc
     1981 aaatgcttac aaaagtggcc agaactgagt gtgcaggctg aaaaattaat agcgcactat
     2041 cctgttttga cacctggtta tcatcattta acatttagta acaatcagat tactttaaca
     2101 ttaatgctag gagacgtgct ggagtgctat gagcaattat tattctgtgg ggacattaac
     2161 cttgaacatc aattgagaga atcctatgtt aatgcttggt acctggatgg attttcacca
     2221 tcaaaaaacc aatccatgtg gtctgacaat ctgttcactg tcattgccat gctatctaaa
     2281 gaaagcacca cagtagccac ctattccgct tccagtatcg tcaaaactgc tttaaccaat
     2341 gcaggatttg ttattgaaaa aagaaaggga tttggaccca aacgacacat gatatgcgct
     2401 cattatgaaa aagcttattc aagttcaaaa aaaaatcgcc ataccccgtg gcacataaac
     2461 tatcctgtga caaaggacga aagaacagcg ctcattgttg gaggagggct tgcaggttgt
     2521 tttattgcaa atagcctcgc taagcgtgga tgggaagtga ccatattaga agaaaaagag
     2581 aaagtgggat gtggtggctc agcaaatcaa caggctgtat tgttccctaa gttatccacc
     2641 tataaatcac catttactca atttatgctt tattcctttc tttatgctaa tgacgtttac
     2701 aaggaacttt tgaaatatta tgaccttggt gaattaaaag gctctttgct gttagctcat
     2761 aacgagaagg aaaaagcaaa ccagcaaagt cttattcatt ggctagaatt ataccctgaa
     2821 ttagggcaat tagtggatga aaagcagtct tctgaattat cagggatatc tttgccatgc
     2881 ggtggtttat ttattccatc ttccggctgg attaattccc ctgaactatg cgatatttta
     2941 attgataata aaagaatttc tctaatcact ggcaatagag ttcaatctat taactataat
     3001 caaaaaaact gggtagttaa tggcattgaa gcctcggtac taattttggc aaatggacag
     3061 caagtaaatt attttcacga aaccaatcat ttgcctgtaa aagccattag aggacaaatg
     3121 actacgattc aatcaacaca agaaagtacc aaattgaaga ttcctttatg tgccgaaggg
     3181 catgttttac cagctttaaa caatagtcat aaagtagggg caagttatga tattggcaca
     3241 tctgagcctg agcttaatgc tctggatgat caattaaacc ttgcccgatt aaaacgtata
     3301 gcgcctgata taatgtggtc ccaaaacgtg ttagaccatt gggctggtat acgagctgca
     3361 tcaccagatt acttgcctat agtgggaccc ctccccaatg cgcttgaatt taaagaaatt
     3421 tattcagaat taaaatctaa ctccaaacga tggatagctg aagcagctcc ttgctatcca
     3481 aatctatatg tgtgtgcggc ttttgggtca agaggattaa caacaatacc tctcgcaact
     3541 gaatggttag ctggtttaat taataaggaa atttcaatac tgcccagaaa attgatacaa
     3601 gctatatcgc ccgcccgatt cttaagaaaa aaaattatac agggaccata agagatatat
     3661 tgaagatata ttgataattc gacttaactt catctttctt tatcagttat aattttaatt
     3721 tttatgtgag ctgattgtgc agaatattga gttttatcaa atagatgctt ttacggacaa
     3781 agtcttccat ggtaatccgg ctgcggtttg tattctcgat gattggttaa atgatgagct
     3841 attaaaagcc attgctgcgc aaaataattt ggcagaaacc gcttttatta taaaagatga
     3901 tcaagatttt catattcgct ggtttactcc taatggggaa attaatctat caggacatgc
     3961 aactttggca gcagggtttg ctttacttga gtcaaataaa ggacctagta attctattgt
     4021 ttttcaccac tccagtggac agatttcggt agaaaagaga aaggaaaagt ttgttctgaa
     4081 ttttccaaga ataggttata agaaaactct ttcaccagac ccaattgtat cactattgga
     4141 tcaacctgct atagaagtat ttgaaacaga ccttgattat ctagttttac ttgaacatga
     4201 gtcacagatt cgaaatttac aaatcgattt atttgcttta gcaaaactcc ccaaaagagg
     4261 aatcattgtg acctcaacaa caacagaagc cgatttctat tcccgatgtt tttatccaaa
     4321 acacagcatt gcagaagatc ctgttaccgg ctcagcacat tgcatattag ctcctttttg
     4381 ggcagaaaaa ctcaataaga acagcctata tgctatccag ggattacaca gaaaagggga
     4441 gttatattgt gagctcgaga ggaatagggt attgatttcc ggccattgtc gatggtatct
     4501 acgcgggcat ttagtaattt aataaaatcg attgagatgc attcatcatt gaagcataat
     4561 ataaaaccca tttaggagcc aatggagcat gccattccaa aaggaattct cttcaggaga
     4621 ataaggtaaa atagtcaacc gcttcaaagt tcagtaataa taaaaacatg cgcagcaatt
     4681 ttgactgcac tataaataaa aactagacca actgatggag gaaaaaaatg agtagcaaaa
     4741 gtggaggttt agccggtgta gttgctggtc aatcagcaat tgccacagtt ggactggcag
     4801 gcaaaggatt aaattataga ggctattcaa taaatgatct ggctgaatat gcaagttttg
     4861 aggaagttgc ttatctgctt cattatggtg aacttcctac ccaaaaggaa cttgatgtat
     4921 acataaaaaa gctggttaac ctaagacata tacctgatgt gcttaaaacg gttttgaagc
     4981 ttatacctaa aaatactcat ccaatggatg tactgcgaac agcatgttct tttttaggca
     5041 ctatcgaacc tgaagagaat tttaagcaac aatatgaaat agctgatcgt ttgctagctc
     5101 ttttcccagg aataatgtgt tattggtata catatcattt caaaaataag gaaatctctg
     5161 gcttaagtga agaagacaca acaggaggac actttctggc gttattacac ggacgcaagc
     5221 ccagcaaaat agaaaccgat atgatgaatg tctctttgat cttgtatgct gaacacgaat
     5281 ttaacgcttc tacctttgct gcaagagtta cagcggcaac actggcagat ttttactcag
     5341 cgatcacaag tgctatagga acattgcgtg gtcctttgca tggtggcgct aatgaagcag
     5401 ctatggggtt aattgagcgc tttaaaaccc ctgatgaagc agcgataggc ataaaacaaa
     5461 tgcttgccag caaagaactg atcatgggat ttggacatcg agtttataca acttgtgatc
     5521 cacgctcaga tattattaag aaatggtcat ttagattagg tgaagaaaag gggaacctac
     5581 ttttatacca tatttcagaa cgtattgaag aaatcatgtg gcaagagaaa aaactatttc
     5641 ccaacctgga tttttatagt gcatccgctt atcattactg cgatatacca actcatttgt
     5701 ttaccccaat ttttgtgatg tcacgcatca caggatggtc tgcgcacgta tttgagcaaa
     5761 gagcaaataa taaactaata agacctactt cagaatatat aggcccagat tccaggccat
     5821 ttcctgctat tgaaacacga ggttaatatg cacaattatg tagaggataa tgttaagcca
     5881 gactatgatc aggttataac agatatagct gattttgtat taaataaaga aattgatagt
     5941 gtgcaggcct atgagactgc gcggttatgc ttaatggata cactcggttg tggcatattg
     6001 gcccttaact ttcaggaatg taccaaatta atgggaccta tagtaccggg agcaactctt
     6061 cctggaggcg cgagagtacc aggtactaat tatgaactgg atccggttca agctgctttt
     6121 aatatcggca cgatgatacg atggctagac tttaatgata cttggctcgc tgcagaatgg
     6181 gggcaccctt ctgataattt gggtgctatt ttggctgttg ccgattattt gtgccgggaa
     6241 aattgtaatc atggacgtcc gcccatcttg atgcatgatg tacttacggc aatgattaaa
     6301 gcacatgaaa ttcaaggttg tttggccctg gaaaatagct tcaatcgcgt cggtcttgat
     6361 catgtcatat tagttaaagt agcgagtgcc gctgttgctg ctcacttgtt tggagcagat
     6421 cgtgatatga tgctaagaac tctctcgcag gtttttgtgg atggacaaag cttgagaaca
     6481 tatagacatg ctcccaatgc aggttcaagg aaatcatggg cagcaggaga tgctacctcc
     6541 cgtgcggttc gtttggcttt aattgccaag gcaggggaaa tgggatatcc aagcgccttg
     6601 accgcacaaa catggggatt ttatgacgtg ctatttggta aaaaacaatt caagtttcaa
     6661 cgcccttacg gcagctatgt gatggaaaat gtgttattca agttatctta tcctgctgaa
     6721 tttcatgcac aaaccgctgt cgaatgtgcc gtaaaattac atcctatagt aaaaaataga
     6781 ttagaggata ttgctagaat tgaattaatc acacatgagt cagcaataag aataataagt
     6841 aagcaaggtg ctttatataa tcctgccgac agagatcact gcttgcaata tatggttgca
     6901 gtaggacttc tctttggtga tttaaaggca gagcattatg aagacgacgt tgccgcagat
     6961 cccagaatag atgctttacg agctaaaatg catgtaactg aaaatgaacg gttttcacgc
     7021 gattatcatg atccggaaaa acgctctata gctaacagca tgagaatcat ctttaaagat
     7081 agctcagaaa gtgaattaat aactatagaa taccctattg gccataaacg gagacgagaa
     7141 gaggggattc ccgttttaat gtctaaattc aagcgtaatc tgagtacaag attctcaact
     7201 gacatggttg aaaagattat ttatgccatg agcgacacca acacgctatc atctatgaaa
     7261 gtaagtgatt ttatggccat gtggatagta aattaaaaag actaactaat acccacgctc
     7321 aatctgttat ctgtactgac ttttagcaaa gaccaggagg gggctaagcg tatagcaaag
     7381 ccaaccctcc ttactaaatc gtggccttca aaaataggtt ttatattttc ctttgtaaac
     7441 cggcaatagc aagaaccttt gtcgatattt cttctatgga atattttgtt gaattgatat
     7501 aaggaatatt ttctcgttgg tacatggctt ctacttctgt tacctctaat ctgcactgct
     7561 ctgcagaggc atatttgcta tttggtctac gttcagagcg aatttgctgc aaacgttgtg
     7621 cgtcaattgt tagtccaaat aatttttgtt tataaggtct taatacctct ggtaagcgaa
     7681 aacccaccaa atcttcttct gtaaaaggat aattggcagc taaaatgcca tattgcagtg
     7741 ccatatataa gcaactggga gttttgccac aacgtgaaac tcctataaga ataatatctg
     7801 ctttatcgta accacgcgtt tttattccat cgtcatgtga aagggcaaaa tcgattgctt
     7861 caattcgatg ggaataggat ttcacattcg caaccccatg agtcctgcct actgtataag
     7921 atgatttctc ttcaagctca ttttccaatg gtccaataaa aatactaaac aaatcaaaaa
     7981 cacaggcatg cgccttttta atggcttgtc taatttctgg atcaactaaa gtcatgaata
     8041 ctaagggctt ggttccttgc tctgaaaaac attgattaat tcttagtaca acactttctg
     8101 ctctgtgagt tgaatctatg tagggtattg ttattttttc aaattgaata ttctcaaatt
     8161 gagtaatcag actattacct aatgtctcag ccgtaattcc tgtaccatcg gaaagcataa
     8221 aaacataacg tttcataagt ggccttttta attcattgat taataaataa ataatattta
     8281 aattataaat gggaatagca attgtactta ttaatagcca tctgctatct taaaaataat
     8341 aatattacaa atccaatcat gggaacacta tgcagcataa tatatacgag aatagtaaac
     8401 tacatattct agggattatc tgccttgtaa cttccctggg tctatttttc tttagccttt
     8461 atattgtacc tttcttaatt tggcaattgg attacgatat ccccgatttt attagcaaca
     8521 tgatagcata cggggaagat gagtattatt tcagccctat tactagcaaa acactggttt
     8581 ggcttatctt ttttacacca agtataatta tgggttatct ttcttattac atctctaact
     8641 atattgataa aaactattta gaaccccagg aaaaaccttt agaaagtatg caagaaaaat
     8701 caccttccaa acctgccggt cgtgggagag attcgactat attgggattg aaaatcattc
     8761 tcttaatggt tttaattgtt gcaataattt ttttactaca aatattattt gacctaacag
     8821 cttaggtgag ggagtagggc atgtttatct ttaaacgatg gaaaataaga aaaatcacaa
     8881 aaagaatcaa ggctatgcaa gccaatagag taagtaatca acctggcgat gaagttctta
     8941 aaaaag
//
LOCUS       DACTJE010000132         8930 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.132, whole genome shotgun sequence.
ACCESSION   DACTJE010000132 DACTJE010000000
VERSION     DACTJE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8930
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.132"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(16..531)
                     /locus_tag="I9760_002289"
     CDS             complement(16..531)
                     /locus_tag="I9760_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5044049.1"
                     /translation="MSNVSIRKATPLDAEAIASVHVHSWKKAYQQFIPEIVLNSLSIA
                     ERTQLWNELLNNGVTVLVLEIDNQIIGFASICTFRDDIEDNSKGEISAVYIHPNFWHQ
                     GFGKKLSLAALKNLKELGYSEALLWVLSDNSQARSFYEGIGFELNDKSKLAEFYDGGA
                     LLKEVLYKKKL"
     gene            complement(1132..2196)
                     /locus_tag="I9760_002290"
     CDS             complement(1132..2196)
                     /locus_tag="I9760_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4935 domain-containing protein"
                     /protein_id="HAT5044050.1"
                     /translation="MKKIILDTSILRSLSLSKSAEVEALNKLIKYEHVEVYLPYIIYN
                     EYITHLRDEFNQSVIILNREVRKLNKYLYKSRSIKDEFLKAVDQINFDEINEFNYWID
                     KYQIQVATNLDLDPTVVLTDYFNGKLPYRSLKCREDIPDSFIHHEIMKYSEQTKEEII
                     FICHDKKLIKSFENTRISLYDSLKDYVQKPETNKLLSAIKAEFLVELYDYFVENIEDV
                     RERIENDLSTKIDYMEIQSNHIPSDNNEGNIASIEYVDPKDFKIIKDSLYHYGDDLYT
                     FDTVFLADVSVDFYIYKPDYYCLDEEFYTEDHNDHYFSAQKSIIVEFKCNIKVNILID
                     EDDDSVTYENLEINDISFSG"
     gene            2386..3756
                     /locus_tag="I9760_002291"
     CDS             2386..3756
                     /locus_tag="I9760_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044051.1"
                     /translation="MSLALPDFEFSFATRDEFDRKPVAKKLINILNSNIDISPMVIDG
                     SWGTGKTEFCRKTIKLINDEFNETLICAYIDAFEADHTNDPLIPIVAAISELIEDSSK
                     KIAFLNKAKAIFRYGLKASARASVAWLLRSNTETLDQEFQKELEKAVDEGSQNLVDGV
                     ITDYQEAKKDINSLKLILQDLTVEKKLVIFIDELDRCKPDFAIAIIEKIKHVFDVANV
                     KFILVTNYTQMKASVNHCYGLALDAHRYLDKFIKFKFYLPTDKYSNKRKQHVSYHYAD
                     QLIRNSDILKAHSRYYLDSRFDLLNNLIIINELSLREISNFILYLELFHTLKSDQFAP
                     DKWYHLSIFIIFILYFKPELREKIRKKDILASELCKFLGINSHIKAKELTHDKDGVSR
                     DIATLIMIFLNDNSIPGYQSDDEEDFKGWLSKLALSTNVGYLELITYIEQFINDIFNE
                     FQFVTK"
     gene            3786..5126
                     /locus_tag="I9760_002292"
     CDS             3786..5126
                     /locus_tag="I9760_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044052.1"
                     /translation="MAYTKWTYEKLIEEARKYQTKQEFRTKNCSAYAAAKYQGIIEKV
                     CSHMIPAHMSWSDEMLATEAKKYQSKIDFKNGSSGAYQAAHKRELIDQICTHMDNLHP
                     KWTEEKIRDEASKFKTKSEFRASNLGAYKAAWKRGILNDVCMHMIDMKTDWTYEMLLS
                     EAKKYSSRGEFSSISNAYQIAAKRGILDEICSHMEVHHVNWTDEMIFEEATKFKSRSD
                     FKLSSPLAYAAAQRRTILDKACGHMEFKHKYWSNEEIAQEAKKYKTRNELQMRSTAYG
                     IAHKRGILDEVCSHMKYTERVNWTPKLLAEEALKYSTRADFYRNNNNAYVAARRFGVL
                     DDITEHLEYLDRYNTRDVVYLWHAEADIYKVGITSENLGDKRIYDVAKDAGFIPKIVM
                     LENVGTVMAKRIESQILKLGQPLSFRNSFPGSSEFRHFSSTDLNKAIELIKTGN"
     gene            complement(5258..6628)
                     /locus_tag="I9760_002293"
     CDS             complement(5258..6628)
                     /locus_tag="I9760_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044053.1"
                     /translation="MSQDSFQSSYTPVHYGKSASEFSKERGQWLLANVEQQIGWPQKR
                     ALISYRGRKILLLPATSKYYAGVAVITKIGQLDEGKIFVMQFLSALAWYRPSKIDIIQ
                     WTNSATQGSLNQYLRLELTKRPGMTALFFRPTMLPDPKNKNAQLALSLFREGLSLNHL
                     GYSFLSYYKIINMRYPNGATQKQWIRSNLYKLESLKYLSDRLSSLKQKHNDLAYYIYT
                     ECRCAVAHAGGTAPVYDPESLYDEIRFYEIQPIAHALAKIIIEDEFCIKTTNTILKEH
                     LYELEGFHYLIGDKVTKRLKDRESISTTEIQIPHLISLRLWGKRKFRAFEKLSVTVIG
                     INKGIVQLSLTRDGRVIIPLALDFPSEKLIFEPMTGADLSDDGTPNAASQFADTYRFY
                     GEVIANGILEVWDVNSNLILGRLLEYLPVNILPSQTKDNLIKIEKRWRDEAKRRVVAI
                     KSPNGK"
     gene            complement(6743..7072)
                     /locus_tag="I9760_002294"
     CDS             complement(6743..7072)
                     /locus_tag="I9760_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044054.1"
                     /translation="MSKSIEPHQFDLAKDNGEKFILPYAQYFNLEDLNKLIKGVIENK
                     NPWPINQIIHAGGMDEIFRQLYQDKKDISGADDMWIQLWDTILAENANDNFHGFEELL
                     KNNNIIQ"
     gene            7110..8284
                     /locus_tag="I9760_002295"
     CDS             join(7110..7374,7374..8284)
                     /locus_tag="I9760_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611931.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAT5044055.1"
                     /translation="MSIRRKYTKEFKLDAISLVTEQGYTCPEAARSLGINSNILSRWI
                     REAAENNENAFRGNGKLTEEQLEIRQLREEVRRLTMEKEILKKANGLLCERSEIKYSF
                     IAQHKKTWPVGMMCHLMGVTSSGYYSYQKRKSTKPNNEPEHEELLEWVKKISESSKFS
                     YGNRRIRKALNALGYPVGRRKTRRLMREAGIFVRYKKKYKVTTNSNHKQPIFDNVLNR
                     KFQVNEPNRAYVSDMNYIPTHEGWLYLTVVIDLFSRKVVGWNMSSRMKADTVCDALTM
                     AIWQRKPKAGLIVHSDRGSQYASKQYRSLLNQHGLVGSMSKKGDCWDNSVAESFFSRL
                     KDELVHWRNYQTRREAKQDILDYMTMFYNNQKLHSSLDYLSPNQFENRYWGLMKKVA"
     gene            8300..8845
                     /locus_tag="I9760_002296"
     CDS             8300..8845
                     /locus_tag="I9760_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site specific recombinase"
                     /protein_id="HAT5044056.1"
                     /translation="MLDLVKYPYYRCNNKQRGLTKKNISREVLENFFRGSLKEITPTK
                     QIVTLTKAITQEHFNKKMEQRNINLYTLQQELLKIEKQINASTSRFIETTSQVIQRAL
                     EKKIEELEEQKLQILQSIELHSNNKIDFETALMAVLSFMENPYKTWVNGDLKQKKLVQ
                     RLVFIKALPQQRGHFNQVSIN"
BASE COUNT         3046 a         1497 c         1579 g         2808 t
ORIGIN      
        1 catgatatat agagattata atttcttttt atacagcact tcctttaata acgctccgcc
       61 atcgtaaaac tccgcaagtt ttgatttatc atttaattca aagcctatac cctcgtaaaa
      121 gctacgcgct tgactattat ctgataaaac ccaaagtaag gcttcagaat agcctagttc
      181 tttcaggttt tttaacgcag cgagagacaa tttcttacca aatccctgat gccaaaaatt
      241 ggggtgtata taaacagcgc taatttcccc tttagagtta tcctctatgt catcacgaaa
      301 tgtacaaata ctcgcaaacc caattatttg attatcaatt tccaatacaa gtactgttac
      361 gccattattt agtagttcat tccatagttg ggtgcgctca gcaattgata aactattgag
      421 tactatttct ggaataaatt gttggtaagc ttttttccaa gaatgaacat gtactgatgc
      481 aatcgcctct gcatccaagg gagtagcctt tctaatagat acattagaca tgaattaatg
      541 cctcatttat ttagcttttg gtctcgaata atttaatttg ttgtaccatt tttatggtaa
      601 aagggatagt gcaccaatta attaatccct ctgtaacatg gctcaattct atagaaaaga
      661 gcagaaaagg gtcaaatcct gaacatgtat gtcttttgga ctagcgaatg gccatacaaa
      721 tcttatctat atttcttttt tctcgaaaag ccgtctctgc caaactttga tctaagccaa
      781 gaacagactc attttttaca agattaagat ataaaccact agtagtaacc ctagtgttta
      841 caagaggtta tggatgttat tggagtgtct tggatggtta attggtggag gtgtcgtgtg
      901 aaaattggaa ccggttcaat tcccatattt tggagtgggg aaatgagctt ttggcagtgg
      961 ctgcgtagta aatgatctaa aataaccatt gaaatatatg ccaattatag agatgtaatt
     1021 aagttattat caccggtaga gagtagaacc taattactaa gctgttatcc aaatagaagt
     1081 ataacaacaa caagggtcag catggaggct gcattatgag atgtctagac tttagcctga
     1141 aaaactaatg tcattaatct caagattttc atatgttact gagtcatcat cctcatcaat
     1201 taatatatta accttaatat tacatttgaa ttcaacaata atagattttt gggcagaaaa
     1261 ataatggtca ttatgatctt cggtataaaa ctcttcatct agacaatagt aatctggctt
     1321 atagatatag aaatctacag agacatccgc tagaaatacc gtatcaaagg tatataaatc
     1381 atctccataa tggtacaagc tatctttaat aattttaaaa tctttagggt caacatattc
     1441 aatacttgct atattgcctt cgttattatc acttggtatg tgattcgact gaatctccat
     1501 atagtcaatc tttgttgata aatcattttc aatgcgctca cgtacatcct caatattttc
     1561 aacaaaatag tcatatagtt cgacaagaaa ttcggcttta atagcactta ataacttatt
     1621 agtctctggt ttttgtacgt agtcttttaa agagtcatac aaagaaattc ttgtgttttc
     1681 aaaggacttg attaattttt tatcatgaca aataaagata atctcttctt tagtttgctc
     1741 ggaatatttc attatttcat gatgtataaa agagtcgggt atatcctctc tacatttaag
     1801 tgatcgatat ggtaattttc cattaaaata atcggttaag actactgttg gatccaagtc
     1861 taaattagtt gcaacttgga tttggtattt gtcaatccaa taattaaact cgttaatctc
     1921 atcaaaattt atctgatcaa cggcttttaa aaattcatcc ttaatgctgc gagatttata
     1981 caaatatttg tttaacttcc taacctccct gttaagtatt attaccgatt gattaaattc
     2041 atcccttaaa tgagtaatat attcattgta aataatatac ggtaaataaa cctcaacatg
     2101 ttcatattta atcaatttat ttaatgcttc aacctctgca cttttagata aagacaatga
     2161 cctaagtatg cttgtatcta ggattatctt tttcataatg attcctttaa taaagtagcg
     2221 taaaattaag atattattcc cttggcttaa tcttaaatat agtaaaagtt agagcctctt
     2281 taaccttaag caaataatat ttatttgatt catataattg ctcagcttaa gccttgaaat
     2341 tatctaaaca atataattta atggaccctc tcggtcaccc aaccagtgag tttagctttg
     2401 cctgattttg aattttcatt tgcaacccgg gatgagtttg accggaaacc agtggccaaa
     2461 aaactaatta atatacttaa tagtaatatt gatatttcac caatggtaat cgatgggagc
     2521 tggggtacgg gtaaaacaga gttttgccgt aaaaccataa agctaattaa cgatgaattt
     2581 aatgaaacct tgatttgtgc ctatatagac gcatttgagg ctgatcatac aaatgaccca
     2641 ttaatcccaa tcgttgctgc aatatcggag ttaattgagg atagctccaa gaaaattgcc
     2701 ttcttaaaca aagcaaaagc tatttttcgt tatggcttaa aagcttctgc tagagcaagc
     2761 gttgcttggt tactaagatc aaatacagaa acattagatc aggagttcca aaaagagtta
     2821 gagaaagctg ttgatgaagg tagtcaaaat cttgttgatg gagttattac agactatcaa
     2881 gaggctaaaa aagatatcaa tagcttaaaa ttaatcttac aagacttaac tgtcgaaaaa
     2941 aagcttgtca tctttattga tgaacttgat agatgtaaac ccgattttgc aatcgcaata
     3001 attgagaaaa ttaaacatgt atttgatgtc gcaaatgtta aattcatttt ggtaacaaac
     3061 tacacccaga tgaaagcctc tgtgaaccat tgctatggct tagctcttga tgctcataga
     3121 tatcttgata aatttattaa attcaaattt tatttaccta cggataagta ttcaaataaa
     3181 agaaaacaac atgtgtctta tcattacgca gaccaattaa taagaaatag cgatatatta
     3241 aaggcacaca gcaggtacta tttagactca agatttgatt tattaaataa tttaatcata
     3301 atcaacgaac tttctttacg agaaataagt aattttattt tgtacttgga gttatttcat
     3361 acattaaaga gtgatcaatt tgcacccgat aaatggtatc acctttcaat tttcattata
     3421 ttcattttgt attttaaacc agagctgcgt gaaaaaataa gaaaaaaaga tatattagct
     3481 tctgaactgt gcaaattttt ggggattaat tctcacatta aagctaaaga gctgacacat
     3541 gataaggatg gagtatctcg agatatagct actttaatta tgatctttct aaatgataac
     3601 tcaattcctg gctatcaaag tgatgatgaa gaagacttta aaggatggct ctcaaaattg
     3661 gcattaagta ctaatgttgg ttatttagaa ttaatcactt acatcgagca attcatcaat
     3721 gacattttta atgagttcca gtttgttaca aaataaaaaa atgaaaaact cggataagta
     3781 atactatggc atacacgaaa tggacatatg aaaagctgat tgaggaagct agaaaatacc
     3841 agacaaaaca agagtttcgt accaagaatt gctcagctta tgcggcagcc aagtaccaag
     3901 gcattataga aaaagtttgc tctcatatga tcccagccca tatgtcttgg tctgatgaaa
     3961 tgttggcgac tgaggcgaag aaatatcaat ctaaaatcga cttcaagaat ggttcttccg
     4021 gagcgtatca agcagcccat aaacgagagc ttatagatca aatctgtact catatggata
     4081 acctacatcc aaaatggaca gaggagaaaa taagagatga agcttctaaa tttaaaacga
     4141 aatcagagtt tcgtgccagt aaccttggtg cttacaaagc ggcatggaaa cgcgggattt
     4201 taaatgatgt ctgtatgcat atgatcgata tgaagaccga ttggacgtat gaaatgctct
     4261 tatcggaagc taagaaatat agttcaagag gcgagtttag ctctattagt aatgcttatc
     4321 aaatagctgc aaaacgtggc attttagatg agatatgttc acacatggaa gtacatcatg
     4381 ttaattggac tgacgaaatg atttttgaag aagctactaa atttaaatca cgaagtgact
     4441 ttaaactatc aagtccttta gcatatgcag cagcccaaag aaggactatt ctggataaag
     4501 catgcgggca catggaattt aaacacaaat attggtcaaa tgaagagata gctcaagagg
     4561 ctaaaaagta taagaccaga aatgaactcc aaatgagatc tacagcctat ggaatagcac
     4621 acaaaagagg tattttagat gaagtttgtt ctcatatgaa atacacagaa agagtaaatt
     4681 ggacaccgaa attactagca gaggaggcat taaaatattc aactagagca gacttttaca
     4741 gaaataataa caatgcatat gtggcagcta gacgttttgg agtactagat gatattacag
     4801 agcatttaga atatttagat agatataata cgagggatgt ggtttatctg tggcatgcgg
     4861 aggctgacat ttataaagta ggcataacta gcgaaaatct aggtgataag agaatttatg
     4921 atgttgccaa agacgctgga ttcataccta aaatagttat gcttgaaaat gtaggtactg
     4981 taatggctaa gagaattgaa agccaaattc ttaaacttgg tcagccttta tcatttagaa
     5041 atagctttcc cggttctagc gaatttagac acttttcttc aacagactta aacaaagcaa
     5101 ttgaacttat taaaactggc aattgaacgt ttcgggtgta aaagtgattc atgttggtca
     5161 gcaatagtgg cgaaattgtc agattagatt gtaatcaata attcatggtc aggtcatatc
     5221 cagcttttta cacttaaata aatatgcatg ccttttctca tttcccattg ggggatttaa
     5281 tcgcaacaac tcttcttttt gcttcatccc tccatctttt ttcaatttta atcaaattat
     5341 ctttcgtctg cgaaggcaat atattaactg gtaaatactc aagtaatcga cctaagatta
     5401 aatttgaatt tacgtcccaa acctccagaa tgccgtttgc aattacctct ccataaaatc
     5461 gataagtatc agcaaattgt gatgccgcat ttggtgtgcc atcatctgat aaatcagcac
     5521 cggtcattgg ttcgaaaatt aatttttcac tgggaaaatc taatgctaaa ggaatgataa
     5581 ctctaccgtc acgcgtcaaa cttagttgaa caataccttt gttaattcct ataactgtaa
     5641 cagataattt ttcgaacgcc ctgaatttcc ttttacccca aagacgtaag ctaattaagt
     5701 gtggaatttg aatctctgtt gttgatatac tttctctatc tttcaatcgt ttagttacct
     5761 tatcccctat aagataatga aagccttcta attcatacaa atgttcttta agaatagtat
     5821 ttgttgtttt aatacagaat tcgtcttcta ttataatttt tgctaaagca tgtgcaattg
     5881 gttgaatttc ataaaaacga atttcgtcat agaggctctc tggatcataa acaggagccg
     5941 ttcccccggc gtgcgccaca gcgcatcggc actctgtata aatataatac gcaagatcat
     6001 tatgtttttg ttttaaacta cttaatcgat ccgacaaata tttcaaactc tcgagtttat
     6061 atagattact tctaatccat tgtttttgag tagcaccgtt aggatatctc atgttaataa
     6121 ttttataata gcttaagaat gaatacccta aatgatttaa cgacaatccc tctctgaata
     6181 atgataaagc gagctgggca tttttatttt taggatctgg caacatggtt ggccggaaaa
     6241 ataatgctgt catacctggc cgtttagtta gctctaacct taaatattga tttagagaac
     6301 cttgagttgc tgaattagtc cattgaataa tgtcaatttt gctaggcctg taccaggcta
     6361 atgcacttaa gaactgcatt acaaaaattt tgccctcatc taactgacct attttagtaa
     6421 ttaccgctac ccctgcataa tatttacttg tagccggcaa taataaaatt ttgcgaccgc
     6481 gataagaaat taaagctctt ttttgaggcc agccaatctg ctgctctacg ttggcaagaa
     6541 gccactgacc tctttctttg ctaaattcag aagctgattt cccataatga acaggagtat
     6601 aagacgattg aaaactatct tggctcatat tttttaattc tgtccaatcc tattaaagtg
     6661 attggtatga tattgcagta actattcata atgaagagaa attctatgct ttttcaactc
     6721 catatatagc aaattttgta gttcattgaa tgatattatt gttttttagt agttcttcaa
     6781 atccatggaa attatcatta gcgttctcag ccaagattgt gtcccataat tgtatccaca
     6841 tatcatcagc accagaaata tctttcttgt cttgatacaa ttgacgaaag atctcgtcca
     6901 tgcctccagc atgtattatc tgattgattg gccagggatt tttattttca ataactccct
     6961 taataagttt attcaaatct tctaaattaa aatattgcgc gtatggtaga atgaatttct
     7021 cgccattatc tttggcaaga tcgaattgat gtggttcaat tgatttagac actccagtta
     7081 agagataatt tgtttaattg gaggtcacaa tgtctataag aagaaaatat acaaaggaat
     7141 ttaaactgga tgcgattagt ttagtaactg agcaaggata cacatgtcca gaagcagcaa
     7201 ggagcttggg aattaattcg aatattttga gtcgctggat tcgagaggca gcggagaata
     7261 atgaaaatgc ttttcgtgga aatggaaaat tgacggaaga gcaacttgaa attcgtcagt
     7321 tacgagaaga agtcagaagg ctgacaatgg agaaagaaat actaaaaaaa gcgacggtct
     7381 tctttgcgaa agaagcgaaa taaaatattc gtttatcgcc caacataaga agacctggcc
     7441 ggttggcatg atgtgccatc tcatgggcgt tacttcttct ggatactata gctatcaaaa
     7501 aaggaagagc acaaaaccga acaatgaacc agaacatgaa gagctattgg agtgggttaa
     7561 aaaaatctca gaatccagca agttttcgta tggaaatcgc aggataagga aagcactgaa
     7621 tgctcttggt tatccggttg gccgcagaaa aacccgtaga ttaatgcgtg aagcgggtat
     7681 ttttgttcgc tataaaaaga aatacaaagt gacaacaaac agcaatcaca agcaacctat
     7741 ttttgataat gttttaaaca ggaaatttca ggtcaatgaa cccaatcgtg cctatgtgtc
     7801 cgatatgaac tatatcccaa cccatgaggg ctggttatac ctgacggtgg taattgattt
     7861 gttttcccga aaagtggttg gttggaacat gagttcgaga atgaaggctg atacagtttg
     7921 cgatgcatta acaatggcca tttggcagag aaagccaaaa gcaggcctta ttgtgcattc
     7981 cgacaggggc tcacaatatg ccagtaaaca atatcgcagc cttctcaatc aacatggcct
     8041 tgtgggcagc atgagcaaaa aaggtgactg ctgggacaat agcgttgctg agagtttttt
     8101 cagccgcttg aaggatgaat tagttcattg gcgtaattat caaaccagac gggaggcaaa
     8161 acaagatatt ctagattaca tgactatgtt ttataacaat caaaaattgc attcgtcttt
     8221 ggattatcta agtccgaatc aatttgaaaa tcggtactgg gggttaatga aaaaagtggc
     8281 ttaactgggg tgtacaaatt tgcttgacct cgtcaaatac ccctattatc gttgtaacaa
     8341 taaacaacgt ggcttaacga agaaaaacat tagtcgagaa gtattggaaa atttttttag
     8401 aggtagttta aaagaaatta ctcccactaa gcagatagtc acgctcacta aagcgattac
     8461 acaagagcat tttaataaaa aaatggaaca acgaaatatt aatctttaca ccttgcagca
     8521 agagttactc aaaattgaaa aacaaataaa cgctagtacc agcaggttta ttgaaactac
     8581 tagccaggta atacaacgcg ctttagagaa aaaaattgaa gaattagaag agcaaaaatt
     8641 acagatatta caatctatag agcttcattc taataacaaa attgattttg aaaccgcact
     8701 tatggctgtt ttatcattta tggaaaaccc ttataaaaca tgggttaacg gtgatttaaa
     8761 gcagaaaaag ttagttcaaa ggctggtatt tataaaggct ctaccccaac aaagggggca
     8821 ctttaatcaa gttagtataa actaacctct tgatttcgcg atcaaaaagg agattaaagt
     8881 gccgagacat gatgttatcc taaatttgcc tggttttact ataaaaaaag
//
LOCUS       DACTJE010000133         8924 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.133, whole genome shotgun sequence.
ACCESSION   DACTJE010000133 DACTJE010000000
VERSION     DACTJE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8924
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.133"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..557)
                     /locus_tag="I9760_002297"
     CDS             complement(<1..557)
                     /locus_tag="I9760_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1158 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044057.1"
                     /translation="MIIYLSIWLIIGMKELIAFIHDLYKDRTEKSVKFESKGRVIVSP
                     LMSKDDAQELFKSLKEILPEEARLKVRKSKQEPDQFRVVLFNPEKFFSFYAEHAAELL
                     NRFKPLPTSSSGEEYCKWKYNPLSHLIEYTVPFSILDSDTKAKVTDTHEEAIQSKVNQ
                     YNEKLRSIYPNLKIIGTNSVEDSSP"
     gene            complement(669..1433)
                     /locus_tag="I9760_002298"
     CDS             complement(669..1433)
                     /locus_tag="I9760_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5044058.1"
                     /translation="MGYSIINKNRSIKIILMIFIGITTFVKAYAYSPDTSNPIQRNRS
                     LAEVNSRHPGEVIVLIHGLMRTYVSMKPLKAYLEAQGYQVYLYKYPSARYNIQEHGVY
                     LNQFITTILENNPAIKIHFVTHSLGGIIVREALSKLSQKQLKHVGYLIMLAPPNQGSK
                     LAKFSIKLFPMISYFIKPLAELSSEQTSYVHRVPVPDIKMGIIAGRFDAKVPPDYARL
                     QGQTEQVIINNTHTFIMYDSKVKKLVLSFLEKGKFE"
     gene            1839..3035
                     /locus_tag="I9760_002299"
     CDS             1839..3035
                     /locus_tag="I9760_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HAT5044059.1"
                     /translation="MLEYLTWITANIVLVSNSIFGTPHPVHAQLQVPQCLAAKMTTAH
                     DVLAENEQFKIIDLPGSDIENVTLLAEQVNCGDFIDVTDKFSDTLLTAKRKSAKNILQ
                     SSALTALKKSQEDKNIYEIKHQKEVNAAIKEVVADNIWQTLNHLTSYSNRSATKDTGV
                     AAANWLKSEFETMAMEYNRRDTATFLVKAGWYKQPSLVTVIGKELKTPAIVIGAPMDT
                     LDGNMPGADEGSGAAAIMESTRILLASKLKFKHPIYIIWYAASDRNLAGSQYVVQYFQ
                     EKSIPVKAVLQLNKTGFRANSDDSTMWVFTDNTNNDLNQYIAKLIKNYIHTPVNFSQC
                     NYECGDHISWSQENIPVTYAVESDFKNLNPYIGSSSDTMDFLNLEHIANFSKLAIAFS
                     IELGSK"
     gene            complement(3093..4772)
                     /locus_tag="I9760_002300"
     CDS             complement(3093..4772)
                     /locus_tag="I9760_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate decarboxylase"
                     /protein_id="HAT5044060.1"
                     /translation="MCSIGEYLAKRLEELNISEYFAIPGDYNLILLDEVLKNEKIKMI
                     NCCNELNAGYAADGYARVKGVSALFVTYSVGGLSVVNAVAGAYAENLPILVISGGPNT
                     NSIQDAEILHHTLATEDNLYVRDIFSRITAHVVCIRKPEQAAMQIDEAIAIAIAKKKP
                     VYIEIACNIANYPISKPTQRAFNTYKISDSSSLAAAVEHAAEKLNSALKPSLIVGSKV
                     RHCNAINSVSELAKCTGYAMAAMPDAKGFISEQHPNFIGIYWGPVSSSGCAEIIDSSD
                     AYLLIGPNENDYTTVGYVWGINPHKSIKTTKGGVIIGETVYTNIFMNDFLRELSKKLK
                     FNDASYKAYKRIAEETVVYPELDQPDAPLSSRYLFGQIQKMLSDKKALLAETGDSWFN
                     CMRLSLPEGCPFEIQMQYGSIGWSVGALLGMQAALHNQKRVIACIGDGSFQMTAQEIS
                     TMIRYGYKPIIFLMNNACYTIEVQIHDGPYNVINNWRYAELVNVFRGEQSNAKSFVVK
                     TNKELLTAIKQAEKTDALCFIEVFLDKDDCNKNLLEWGSRVANYNSRPPRR"
     gene            complement(5012..6094)
                     /gene="ravQ"
                     /locus_tag="I9760_002301"
     CDS             complement(5012..6094)
                     /gene="ravQ"
                     /locus_tag="I9760_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavQ"
                     /protein_id="HAT5044061.1"
                     /translation="MMRMFSVRRNLSAVMRKDLSLTSRPLLQAPLLKSTVNPQNTLKT
                     KELKERKFTETLPDSRFFSKLKLAEQDQFSHRKRKRFNYQKNFSAALSLPYSRKELDV
                     HRDEEDALLSMVKQYVIEHANRRVDLFFYTLIAYYNTEIVPTNGYTDLQHGRGRNTNR
                     GITITEACHSSLTPSLLDSTIYQNKGRLKHKSLLSGTHFMDSLNATVELPPFVNDFDD
                     ILEKACRKKCLEILHEVSLGRINPMEGLTLFLKIMQDILIKFKEEAASENQSALLQNS
                     LIRKKFISPNLVDLVINGTLSTTFSDSTQSASDDYIQLLLRMTSEEKALCEKDEKGKI
                     KLYLAKIMDIQNEILTSKNGEFVPGL"
     gene            6580..8067
                     /locus_tag="I9760_002302"
     CDS             6580..8067
                     /locus_tag="I9760_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT5044062.1"
                     /translation="MQNKLLQFICIKKLYKQKQSYKSIIKFASLPLAKKQIMCSIALL
                     FVIKLSWATHHYDTIIIGAGVSGLTAAHHLHKAQQKVLIIEAKNRLGGRVYTSYDWGF
                     ATDLGASWIHAIENNPLMPLIGKQSIIINTYSNSDPVAMLNNYALYDSEGKPVSKQTQ
                     TLFSSLTKEFLRYCQTRNQMISFAQNLTTFAKQKKLTSEQLALLSYALENIYTYEFAD
                     NLTKLSRNVHSASEASIASGKNALVPEGYFQLFRPLTQHVPIHLNQIVSQINYGADGV
                     NIITQHEKYHANQVIITVPLGVLKANAIKFHPALPKDKRTAISQLGMGSYEKLYLLFD
                     KVFWDKDKEWIGMLPQNEQEAFNIFNYYKYTKKPVLIVFTSGKLAHDMEKEHLTEWVM
                     QHLRRIYGSNIPKPIKNKKTHWGSDPFTRGSYSYLPVNVDKSVIGILAQPVANRLYFA
                     GEATSTTDPSTVHGAYLSGIRAAEEVLASIKHSGKNREKDKAVNS"
     gene            7967..8923
                     /gene="ravP"
                     /locus_tag="I9760_002303"
     CDS             7967..8923
                     /gene="ravP"
                     /locus_tag="I9760_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavP"
                     /protein_id="HAT5044063.1"
                     /translation="MALIYQGYAQQKKYWPASNTLEKIEKKIKLSTHKPILILEKVMY
                     TKRDNHLLINKEEEQEENQKTVFVPQTNSLALPETQSYFCTAEQCSLNNPYAPAQGYT
                     RPDSLHFKTTPINSEIYEQVFKVSEETVKTETQFANRAPGHLEKEENRSGDWSGFIPD
                     ELYQMMMEAAKQGIFVSFLLTLTDEIINDYLKNHHYSQQQINWISQGIRALTLIAIGT
                     SPGIALGLPISTLLLIEQLGFDKTNVNYLTTSSVVAWGLLSSPFNLLNTSLVMASSIG
                     ASVLTSKITQSSYQLVRNRFFSSKPEKEELEQENILQKTIQQ"
BASE COUNT         2899 a         1738 c         1555 g         2732 t
ORIGIN      
        1 tcaggactag agtcttcaac tgaattagtt ccgataattt tgagattagg gtaaatcgac
       61 cgtaacttct cgttatattg atttactttt gattgtatag cctcttcatg agtatctgta
      121 actttcgctt ttgtatcaga atctagaatt gaaaacggaa cagtatattc tattagatgt
      181 gataaaggat tatatttcca tttacaatat tcctctcctg aagaagatgt cggtaatggt
      241 ttaaatcgat ttaacagttc tgcagcatgc tcggcataaa acgaaaaaaa cttttcagga
      301 ttaaagagaa cgacacgaaa ctgatctggt tcttgctttg acttacgtac ctttaatcgt
      361 gcctcttcag ggagtatttc ttttaatgat ttaaataatt cctgggcatc atctttactc
      421 atcaaaggag atacaataac tctccccttg gattcaaatt ttacagattt ctcagtacga
      481 tctttataca agtcatgaat aaaagctatt aattctttca tgccaattat caaccatatt
      541 gataaatata taatcaaatt tagatataat taattaacat gtcaattttt acacttggat
      601 ttttgacaat aaaagctgga tagaatactt aattaagcca atactagccg ctgactataa
      661 ctaaaaaact attcaaattt ccctttttct aaaaagctca gcacaagttt ttttactttt
      721 gaatcataca taataaaagt atgcgtatta ttaataataa cctgctctgt ttgtccttgc
      781 agacgagcat agtctggagg aactttagcg tcaaaccgcc cggcgattat ccccattttg
      841 atatcaggta caggaacacg atgcacataa gatgtttgct cagaacttaa ttcagccaaa
      901 ggttttatga agtaggaaat catcggaaat agtttaatag agaattttgc cagcttcgaa
      961 ccttgattag gaggagccag cataatgaga taacctacgt gttttaattg tttttgagat
     1021 aatttggata atgcttccct tacaataatg ccaccaaggc tatgggtaac gaaatgaatt
     1081 tttattgccg gattattctc aagtattgtt gtaataaatt gatttaaata aactccatgc
     1141 tcttgaatat tgtatctggc ggaggggtat ttgtacaaat agacctgata tccctgagcc
     1201 tccagataag cttttaatgg cttcatactc acataggtac gcattaaacc atgaattaat
     1261 acaataacct ctccaggatg cctggagttt acttctgcta atgaacggtt tctctgaatt
     1321 ggattactgg tatcagggga gtaagcataa gccttgacaa atgtggttat tccaatgaat
     1381 atcatcagaa ttatttttat gcttcgattt ttattgataa tggaataacc cattattatc
     1441 tctattattg atttgaataa aattattctt tctatagata aacttaaaat gattattaat
     1501 caagaatatc cattcaatta ttgcattgag ttgagtgcaa actaattcag catgaaaata
     1561 aaaccagcaa atgagcttat gattgcctta tcagataaaa taaaccacat atttcccaac
     1621 taaaggctaa gaaatttttc aagaataaag gtgttaattt tgttaatttt gtagtatatt
     1681 tgtccacgct attttggaga taaaacacat gacaagtaaa ttggtcattt acaatataca
     1741 gaaaaaataa cgaactctcc aaaattattt tttaatactt ggaataatgt gctttgccca
     1801 tgaatcgata cggattctac agttgtttag gagtgtaaat gcttgaatat ttgacatgga
     1861 ttactgccaa tattgtatta gtgagtaatt caattttcgg aacaccacat ccagttcatg
     1921 ctcaattaca agtacctcaa tgtcttgctg caaaaatgac gactgcgcat gatgtcctgg
     1981 ctgaaaatga gcaattcaaa atcattgatc tgcctggcag cgatattgag aatgtaactc
     2041 ttttagcaga acaagtgaat tgtggcgatt ttatcgacgt aacggataag ttctcggata
     2101 ccctgttgac cgccaaaaga aagtcagcaa aaaatattct gcaaagcagc gcattaactg
     2161 cccttaaaaa atcccaagaa gacaagaaca tctatgagat taaacaccaa aaagaagtca
     2221 atgctgcaat aaaagaggta gttgcagaca acatttggca aacattaaat cacttgacct
     2281 cctatagtaa tcgttcagca accaaagaca caggagttgc tgcagcgaat tggttaaagt
     2341 ctgaattcga aacaatggca atggagtaca atcgcagaga tactgccaca tttcttgtga
     2401 aagcaggttg gtataaacaa ccctccctgg tgacagtcat tggtaaagaa ctgaaaacac
     2461 ccgccatagt cattggcgca ccaatggata ctttagatgg gaacatgcct ggcgctgatg
     2521 aaggtagcgg tgctgctgcc attatggaaa gcactcgtat tttgttagcc tccaagctga
     2581 aattcaagca tccgatttat attatctggt acgctgccag cgatagaaat ttggctgggt
     2641 ctcaatatgt agtccaatat tttcaagaaa aatctattcc tgttaaagcc gtactgcaac
     2701 tcaacaagac agggtttcgc gctaactcag atgattcaac catgtgggta tttaccgaca
     2761 atacaaacaa tgatctgaac caatatattg caaaattaat aaaaaattac atccatacac
     2821 cagttaattt ttcgcagtgt aattacgaat gcggtgatca tatttcctgg agtcaagaaa
     2881 atatacccgt aacctatgca gttgaatctg actttaaaaa ccttaacccc tatatcggca
     2941 gctcttcaga tacaatggat tttttgaatc tggaacatat agctaacttt tcaaagctgg
     3001 ccattgcttt ctcaatagag ctgggctcca aataaaaaca cgtcaaatca ttaatatcag
     3061 ggtaggcgat tcttataatt cctacccaag atctatctac gaggaggacg gctattataa
     3121 tttgccactc ttgagcccca ttccaacaag tttttattgc aatcatcctt gtccaggaac
     3181 acctcaataa aacaaagcgc gtctgttttt tctgcctgtt taattgcagt gagtagttcc
     3241 ttgttggttt taactacaaa agattttgca ttactctgtt ccccgcggaa tacattaacc
     3301 agttcagcgt aacgccaatt attaatgaca ttataaggac catcatgaat ttgtacttca
     3361 atagtgtaac aagcattatt cattaaaaaa ataattggtt tatacccgta acgtatcatg
     3421 gttgatattt cctgggcagt catttgaaat gagccatctc ctatgcaggc aatcactctt
     3481 ttttgattat gcaaagcagc ttgcatgcct agcaaagcac ccactgacca accaatagaa
     3541 ccatactgca tttgaatttc gaaaggacaa ccttcaggca aagacaggcg catacaatta
     3601 aaccaggagt cccctgtttc agccaacaaa gcttttttgt cagaaagcat tttctgaatt
     3661 tggccaaaca aataacggct gcttaatgga gcgtcaggtt gatctaactc tggatagaca
     3721 acagtttctt cagcaattcg tttataagct ttgtatgaag catcattaaa ttttaatttt
     3781 ttacttaatt ctctgagaaa atcattcatg aagatattgg tataaactgt ttcaccaata
     3841 ataacccctc ccttggtggt ctttattgat ttgtgggggt tgattcccca tacataccca
     3901 actgtagtat agtcattctc attaggccca ataagcagat aggcatcact ggaatcgata
     3961 atttcggcgc atccagaaga gctgactggt ccccaataga tacctataaa gttaggatgc
     4021 tgttctgaga taaatccttt cgcatcgggc atagccgcca tagcatatcc agtacacttt
     4081 gccaactcgc tgacagaatt gattgcatta caatgcctaa ccttactacc tacaatcaag
     4141 ctgggcttta gagcggagtt taatttttca gcagcatgct ctactgctgc agccagagac
     4201 gaactgtcgc taattttata ggtgttgaat gcgcgttgag taggcttgga gataggatag
     4261 ttagcaatat tacacgctat ttcaatatag accggctttt ttttagcgat tgcgattgct
     4321 atagcctcgt ctatctgcat ggcagcctgc tctggctttc gaatacaaac gacatgggca
     4381 gtaattcgag aaaaaatgtc ccttacatac aaattatctt cagtcgccaa cgtgtgatgg
     4441 agaatttcag catcctgtat ggagttggta tttgggccac ctgatataac caaaatggga
     4501 agattttcag catatgctcc tgcaactgca ttaacaacac taagtccacc gacactataa
     4561 gtaacgaata aagcagaaac cccttttaca cgggcatagc catcagcagc gtatccggca
     4621 ttcaactcat tacagcaatt aatcattttt attttttcgt ttttcaggac ctcatcgagt
     4681 aaaataagat tgtaatcacc cggtatagca aaatattcag aaatatttaa ttcctccagc
     4741 cgctttgcca aatactctcc aattgagcac atgttaatcc cttattaaat aagaactttt
     4801 taaattatag cacagccaaa aatcagtagg tggcaagtga atacctgcta aaaaatagag
     4861 ttcacaaggc atcaaattgc agatgataat ttaaatattc tatcttggag tatgttgaca
     4921 attcgatcca gaaactacat cccgcgtctt gggcgcggga ttaatggatg ctgtggacaa
     4981 acaaactggt cggcattttt gttaacacat cttaaaggcc aggcacaaat tcaccatttt
     5041 tcgatgtcaa aatctcattt tgaatgtcca ttatttttgc caaatacaat ttgattttgc
     5101 ccttttcatc cttttcacaa agcgcctttt cttctgatgt cattctaagt aaaagttgga
     5161 tgtaatcatc agaagcgctt tgagtgctat ccgaaaatgt ggttgataac gtgccattaa
     5221 taaccaaatc aaccaagttt gggctgatga attttttcct tatcaatgaa ttctgtaata
     5281 gtgcagattg attttcgctt gctgcttctt ctttaaattt tattaaaata tcctgcatta
     5341 tttttaggaa aagggttaat ccttccattg gattgattct tccaagtgat acttcatgca
     5401 ggatttccaa gcattttttc ctgcaagcct tttccaggat atcatcgaaa tcattcacaa
     5461 agggaggtaa ttctaccgtg gcattcaaac tgtccataaa gtgagttccg gaaagaagac
     5521 ttttatgttt taatcttccc ttgttttgat agatagtact atcaagcaat gatggagtaa
     5581 gactgctatg acaggcttca gtgatagtta tgcccctgtt ggtattacgc cccctgccat
     5641 gctgaagatc ggtataacca tttgttggaa cgatttctgt attataataa gctatcaatg
     5701 tataaaaaaa taagtcaaca cgacgattag catgttcaat gacatactgt ttcaccattg
     5761 acagcaatgc gtcttcttca tcccgatgta catctaactc ttttcttgag tatggcagtg
     5821 agagtgctgc actaaaattc ttttgataat tgaatcgttt acgttttctg tgactaaatt
     5881 gatcctgttc tgccaatttt aattttgaaa aaaaccttga atccggtaat gtttcagtaa
     5941 atttacgctc ttttaactct ttagttttca gagtgttttg agggttaacg gtactcttaa
     6001 gcagcggcgc ctggagtaaa gggcgggaag ttaaagaaag atctttgcgc attactgcgg
     6061 aaaggtttct acgaacacta aacattctca tcatggtctt ctcccttaat agccattatg
     6121 tcaccgtagt acctgctcaa agcatcaagc gtctgttttt ccataaagac acttaaactt
     6181 acttagtgag agttcacagt ctggatgatc ttcaaagcca gctccctgtc taagccagaa
     6241 catatactgt ctttttacag tacataataa tctttaaaaa agcaaacatt aaaataactt
     6301 aatattgaat gcttcataca gacaagttaa ttgctatgct gattattatt tacaaatatt
     6361 gatacttttt ttaatatgca tcaattcaat accaaggatt tactgtgtca taccagactt
     6421 ttttctcgac ttgatcgtca taattcaatc ttgtgcaccc gcatgcagcc atgcaaatac
     6481 aaaattctag ccagtcgagg ttagatgcga agcttggttg ggcaaatcaa tttgtccggc
     6541 taaacctata aattaaaata gcgatgtagg aagaaaaaca tgcaaaacaa attattacag
     6601 tttatttgca tcaaaaaatt gtacaaacag aaacagtctt ataaatcaat aataaaattt
     6661 gccagtttgc ctttagcaaa gaaacaaatt atgtgctcca ttgcattact cttcgttata
     6721 aaattgtctt gggcaactca tcattatgac acaataatta tcggtgccgg ggtatccgga
     6781 ttaaccgcag cgcatcattt acataaggca cagcaaaaag tactgattat tgaggcaaaa
     6841 aaccgccttg gaggcagggt atataccagc tatgattggg gtttcgccac tgatttgggg
     6901 gcatcctgga ttcatgccat tgaaaacaac ccattaatgc cgctgattgg taaacagtca
     6961 attattatca atacttacag taactctgat ccagtagcca tgctgaataa ttatgcttta
     7021 tatgacagcg aaggaaagcc agtatcaaaa caaacgcaga ctttattttc aagtttaacc
     7081 aaggaattct taagatattg ccaaactcgc aatcaaatga tctctttcgc gcaaaatctc
     7141 actacctttg caaaacaaaa aaaacttact tctgaacaac ttgccttact gagttatgcc
     7201 ttggaaaata tctatactta cgaatttgct gataatttga ctaaattatc acgcaatgtc
     7261 cattccgcat ccgaggcatc catagcttct ggaaaaaatg cccttgtacc tgaaggctat
     7321 ttccaacttt tccgcccact tactcagcat gttcctattc atttaaacca aatagttagc
     7381 caaattaatt acggagctga tggtgtcaac atcataactc aacatgaaaa atatcatgcc
     7441 aaccaagtca ttattaccgt cccattaggc gtgctcaaag ccaatgcaat caaatttcat
     7501 cctgctttac ccaaagataa gcgcacagca atctctcagt taggaatggg aagttatgaa
     7561 aaattgtact tattatttga caaggttttt tgggataaag acaaggaatg gattggcatg
     7621 ctcccccaaa atgagcaaga agcattcaat atctttaatt attacaagta cacaaaaaag
     7681 cctgtactca tcgtatttac cagtggcaaa cttgcgcacg atatggaaaa agaacattta
     7741 accgaatggg tgatgcaaca tttaagacga atttatggaa gcaacatccc gaaaccaatt
     7801 aaaaataaaa aaactcattg gggcagtgat ccattcacac gtggcagtta ttcctacttg
     7861 ccggtaaacg ttgataagag tgttattggc attctcgccc aacctgtagc taaccgtctc
     7921 tattttgctg gagaagctac ctcaaccaca gatcccagta ctgtgcatgg cgcttatcta
     7981 tcagggatac gcgcagcaga agaagtactg gccagcatca aacactctgg aaaaaataga
     8041 gaaaaagata aagctgtcaa ctcataaacc catcttgatt ttggagaaag tgatgtacac
     8101 taaaagagat aatcatttgc tgattaataa agaggaagaa caagaagaaa accaaaaaac
     8161 ggtatttgtc cctcaaacaa atagtttggc tcttcctgaa acccaaagct acttttgtac
     8221 agcagagcaa tgctctttaa ataaccccta tgccccagct caaggttata caagacctga
     8281 ttcattgcat ttcaaaacaa cccctataaa ctcagagatt tacgaacaag tatttaaagt
     8341 ttcagaagaa actgttaaaa ccgaaacaca atttgcaaat agagctcctg gacacttgga
     8401 aaaagaggaa aatcgttccg gtgattggag tgggtttatt ccggatgagt tatatcaaat
     8461 gatgatggag gctgcaaaac aaggcatttt tgtctcgttc cttctcactc tcactgatga
     8521 aataataaat gactatctaa aaaatcatca ttattcacag caacagataa attggatttc
     8581 tcaaggcatt cgagctttaa ctttaattgc tattggtact tcaccaggaa tcgctttggg
     8641 attaccgata agcacacttt tgctgataga gcagttgggc tttgataaaa cgaatgttaa
     8701 ttatctcaca acaagttcag tcgtagcttg ggggctatta agctcacctt ttaacttgct
     8761 taatacttcc cttgtgatgg caagttctat tggagccagt gtattaacca gtaaaataac
     8821 acaatcatcc tatcaacttg tgcgtaacag gtttttttct tcaaaacctg agaaagaaga
     8881 attagaacaa gagaatatat tgcaaaaaac aatccagcaa taat
//
LOCUS       DACTJE010000134         8874 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.134, whole genome shotgun sequence.
ACCESSION   DACTJE010000134 DACTJE010000000
VERSION     DACTJE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8874
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.134"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..515
                     /locus_tag="I9760_002304"
     CDS             <1..515
                     /locus_tag="I9760_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lpg0189 family type II secretion system
                     effector"
                     /protein_id="HAT5044064.1"
                     /translation="TGFLINSYLDPLTDDAVELLETYLAKNNGSNLLGTPLTFEPAKA
                     LVVALTISAGMKKNPNKPPFVEYKQDRSNFYFKSNYEMRNKLFTDIYQNFFTNDPNKI
                     LPFLDKWIFVNASSVYEAILMDSNFVELQPERIFLMENGEQIFVSNLKYYFTHNCNRY
                     SNHRCLQPQA"
     gene            complement(681..1739)
                     /locus_tag="I9760_002305"
     CDS             complement(681..1739)
                     /locus_tag="I9760_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAT5044065.1"
                     /translation="MTEEVLFSQEGQLGFITLNRPKALNALTLTMIMALQKQLSIWKE
                     DNSIKAVVVQAVPGNAFCAGGDIRWLYNAGRSKDSEQMQFFWHEYRLNHFIHHFGKPY
                     ISLLDGITMGGGVGISLHGSHPVASERFVFAMPETGIGFFPDIGASYLLNKCPGFLGV
                     YLGLTGNKLGPHDARKAGLVKQIVFAEQMQSMIEALKREDLSEDAFNLVDQCISSFAS
                     DAMPTEASQIKPLIDVCFSKPSVELIRESLQSTDGVWALGVDNTLLQKSPLSLKITLA
                     QIQKAKGLSLAECLKMDFDLASHFMKGSDFYEGVRSLLINKDKNPQWRPSSLELVTDA
                     MVVSYFESSSSGLELMIL"
     gene            complement(1742..2518)
                     /locus_tag="I9760_002306"
     CDS             complement(1742..2518)
                     /locus_tag="I9760_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HAT5044066.1"
                     /translation="MNLIEQDIDNNGILTLTLNRPEKLNALSTDVLSALNELFFQAKS
                     NPKVKALLLTGNGKAFCAGADINRLAECNAQTGYEFACQGQEVFRLLETMGKPSLAAV
                     NGFAFGGGCELAISATLRIASNKAMFGQPEVKLGVIPGYGGTQRLARLIGKGRAMDLC
                     LTGRFINAETALNWGLVSEVVAPEDLLTQGKNILNGILSMAPLAVASVMEVIDHGYDL
                     SLTEALHLEAIHFAKVCATEDKKEGVAAFLDKRTADFKGV"
     gene            complement(2535..3689)
                     /locus_tag="I9760_002307"
     CDS             complement(2535..3689)
                     /locus_tag="I9760_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT5044067.1"
                     /translation="MHFNFTDEHLAFREMASEFARNKLAPMADFWDEQGYFPIETLRE
                     AAQLGMAGMVVREDIGGAQLSRLDAALIFEQLATGCIPTSAYLSIHNMVASLVDRYGS
                     QEIRKKWGPKLTSMEVIASYCLTEPESGSDAASLKTRAVKEGEYYVLNGAKAFISGGS
                     VSDVYLCMVRTGDESHHGISCLLIEKNTPGLTFGKLEKKLGWRNQPTCMLYFENCRVP
                     IANRVGEEGMGFKIALNALNGGRVNIASCSLGGALACLRMSQSYMHERKQFGKPLTQM
                     QALRFYFADMLTDYEAARLMVYRAAAAMDNDDPNAPMYCAMGKRLATDVAFRISDKAM
                     QLHGGYGYLRDYQIERIFRDLRVHQILEGTNEIMREIIAKASLDEEYFIE"
     gene            3803..5629
                     /gene="recQ"
                     /locus_tag="I9760_002308"
     CDS             3803..5629
                     /gene="recQ"
                     /locus_tag="I9760_002308"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAT5044068.1"
                     /translation="MGTLAQPSFINPNALAVLKKYFGFDSFRPPQEKIIEDVISGEDV
                     LVLMPTGGGKSLCYQIPAIVRPGVGIVVSPLIALMEDQVTALKLQGIRAAYYNSSLTA
                     EEARNVLNQLHHNELDLLYIAPERLISTSFLDRLSECTISLFAIDEAHCISQWGHDFR
                     PEYACLGLLKTNFPDIPMIALTATADKQTREDIVTKLNYQPKKYVVSFNRPNIHYKVV
                     PKTNAVKQLNLFLQSDTQQSGIIYCGTRHTVEHLTAKLQELGFKARAYHAGLPHAVRR
                     EVQNLFRYDRIDIVVATIAFGMGIDKPNVRFVVHHDLPKSIEGYYQETGRAGRDGLPA
                     KALLLYDAADSARLRSWIMNIPLEEQRHIETNKLNHMLAFAEASHCRRQILLRYFDEP
                     SHTECKNCDVCDNPPQTADATEDAQKFLSCIYRLKQSYGLTHTIDVLRGSLSEKIKQS
                     GHEHLSTFGIGKEKSANYWKHLAWQLIHKDYCLQDMNHFNVLRLTPKAIPLLKGEESI
                     SLTIPNNDLNSSKKKGKQNPSFKPESNPLFEKLRALRRQLADEENKPPFMIFSDATLH
                     EMAQSKPKNSKQLLNVSGVGQHKLARYGHYFLEALNEFSEVE"
     gene            5639..6190
                     /locus_tag="I9760_002309"
     CDS             5639..6190
                     /locus_tag="I9760_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAT5044069.1"
                     /translation="MRKLLRPFYERDTVLVAKELLGKYLVHHDGLEEKIGRIVEVEAY
                     LGQHDLACHSSKGLTKRTKVMFGPAGYAYVYLIYGMYYCMNVVTEKEGIGSAVLIRAL
                     EPIKNIQDRTQGPGLLSKAMRIDSKLNHRDLLSNDFYIAEPNSPTDFTIIEKPRIGVH
                     YAKEWANELLRFYIKDNPYISKT"
     gene            complement(6385..6789)
                     /locus_tag="I9760_002310"
     CDS             complement(6385..6789)
                     /locus_tag="I9760_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAT5044070.1"
                     /translation="MKKLLSASVITLMITGCAAIPADPQSERVIASPNPAPKGCKYVG
                     QVVGNQGNFFTGGFTSNRNLEEGAMNDLKNKASKKGANYIQLVTNRAGVTGSMSGSFD
                     RHGGFMSGGSEQTNVTNLGNAYICPPKAIGLE"
     gene            complement(6845..7048)
                     /locus_tag="I9760_002311"
     CDS             complement(6845..7048)
                     /locus_tag="I9760_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HAT5044071.1"
                     /translation="MTDKVYQIVELIGTSQEGIEKAIDNAIKQVAKTHGKLDWFEVVE
                     TRGFIDDNKVKYYQVHLKVGCNV"
     gene            complement(7038..7724)
                     /locus_tag="I9760_002312"
     CDS             complement(7038..7724)
                     /locus_tag="I9760_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAT5044072.1"
                     /translation="MNEWWLVILLVVLAVFACTLITYPLRRNRLVSLLLAPIIFIMVF
                     TGYYFWGDFARWQEYLHRNEAQELAQSMLKSIKSPQELIDKLKAKLDAQPESAKGWYL
                     LGRLYSSQKDNQNASLAFAKAHQLKPEDEQFAVNYAHSLWQLSNQQFNPDILEIFHTL
                     LKNNPRQPDALAMLAMHAYLSHDYEAAINYWQRLLQLAPAQSEEALAIRKAIAKAEKQ
                     INRGGKKSDR"
     gene            complement(7717..8118)
                     /locus_tag="I9760_002313"
     CDS             complement(7717..8118)
                     /locus_tag="I9760_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAT5044073.1"
                     /translation="MRVFHFCFKVILLLLLVSVAFANSMYPLDSARKEAQFNHLLKDL
                     RCLVCQNQDLADSNAQLAKDLRFQVYELVKEGKSDSEIMDYLTARYGDFILFKPPVKS
                     VTLLLWFGPFLFLLIGFIIFWRTCFKRNSHE"
     gene            complement(8118..8651)
                     /locus_tag="I9760_002314"
     CDS             complement(8118..8651)
                     /locus_tag="I9760_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HAT5044074.1"
                     /translation="MKKIGWRVIPIAIFCLLCFFLWRGLSLDPHHLPSARIGKPLPDF
                     ALPELQNPDSTFSVMNFHNQVVLLNVWASWCEACVEEQVFMLQLAREGVPIYGLNYKD
                     NPEHARAWLAQWGNPYRLIGQDSEGKVAIDLGVYGAPETFVIDKKGIVRYRHVGIMNQ
                     KTWLTDILPLMRNLEKS"
     gene            complement(8648..>8874)
                     /locus_tag="I9760_002315"
     CDS             complement(8648..>8874)
                     /locus_tag="I9760_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945971.1"
                     /note="cytochrome c-type biogenesis protein; required for
                     the transfer of heme to apocytochrome c; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HAT5044075.1"
                     /translation="ESAIDVTPFRDIYIALGEPLSEDAWSVRIYYKPLVRWIWAGGFM
                     ILAGGVLALTDRRYYRKRQVTVMSGQEIKI"
BASE COUNT         2726 a         1963 c         1549 g         2636 t
ORIGIN      
        1 ccactggctt tctcatcaat agctatttag atccactaac cgatgatgca gtggaattat
       61 tagaaactta cctggcaaaa aataatggat ccaatctgtt aggtacacca ttgacatttg
      121 agccagcaaa agctcttgtt gttgctttaa ctatctcggc gggaatgaaa aaaaatccta
      181 ataaaccacc ctttgtagaa tataagcaag accgcagcaa tttctacttc aaaagcaatt
      241 atgaaatgag aaataaattg ttcactgata tttatcaaaa tttctttacc aatgatccca
      301 ataaaattct acctttcctg gataagtgga ttttcgttaa tgcctcaagc gtttatgaag
      361 ccattttaat ggactccaat tttgtcgaac tgcaacctga acgaattttt cttatggaaa
      421 atggtgaaca aatatttgtt tccaatctaa aatattattt cactcataat tgtaataggt
      481 acagtaatca ccgctgctta caaccgcaag cttgaagctc ttttgacgaa ggagacattt
      541 ttgggatgag cacattttat taaattatta cttttagata cttccttatg acagcaagca
      601 gataatattt cagactgctt tcaaccataa ggaagtattc atacgggaaa tctattattc
      661 ccccaatcaa aatccaaaaa ctaaagtatc attaattcca atccggaaga tgagctttca
      721 aaataactca ctaccatggc atcagtgacc aactccaaac tggacggtct ccattgagga
      781 tttttatctt tattaatcaa taaagagcga actccttcat aaaaatcact acctttcata
      841 aaatgactgg cgagatcgaa atccattttt aaacattctg ctaaagataa gcccttggct
      901 ttttggattt gtgcaagggt tatcttaagg ctcagaggcg atttttgcag caaagtatta
      961 tccaccccaa gagcccacac accgtctgtg ctctgtaaag actctcttat tagttcaaca
     1021 ctaggcttgg agaaacagac atcgattaaa ggtttgattt gacttgcctc ggtgggcatg
     1081 gcatcacttg caaaagaaga aatgcattga tcaacaagat tgaaagcatc ttctgataaa
     1141 tcttctctct ttaatgcctc aatcattgac tgcatttgct ctgcaaagac aatctgcttc
     1201 actaaccctg ccttcctggc atcatgagga cccaatttat ttccggttaa ccccaaatag
     1261 actcctaaaa accctgggca tttgtttaat aaatagctgg caccaatatc aggaaaaaaa
     1321 ccaatccctg tttctggcat tgcaaataca aaacgctcac tggcaacagg gtggcttcca
     1381 tgtaaggaaa taccgacccc tcctcccata gtaatcccat ccagcaaaga aatataaggt
     1441 ttaccaaaat gatgaataaa atggtttaac ctgtactcat gccaaaagaa ctgcatctgc
     1501 tcagagtctt tactacggcc cgcattatat agccaacgaa tatcaccacc tgcgcaaaaa
     1561 gcattacccg gaacagcttg tacgacgact gctttaatag aattatcttc tttccaaatt
     1621 gacaattgct tttgcagtgc catgatcatg gttaaagtta aagcatttaa agcctttggt
     1681 cgatttaaag taatgaatcc caattgacct tcctgagaaa acagcacctc ttcagtcata
     1741 attaaacccc tttaaaatcc gcggttctct tgtctagaaa cgcagctact ccttccttct
     1801 tatcttcagt ggcgcaaact tttgcaaaat gaattgcttc caaatgaagt gcctctgtga
     1861 gtgataaatc ataaccatga tctatcactt ccattacact ggcaactgct aaaggagcca
     1921 tgcttaatat tccgtttaga atattttttc cttgagtcaa caaatcctct ggtgctacaa
     1981 cttcactgac taacccccag tttaaggcag tctcagcatt tataaagcgc ccagtcaaac
     2041 ataaatccat cgcgcgccct ttgccaatta gcctggccaa tcgttgtgtg ccgccatacc
     2101 cgggaataac acctaatttt acctcaggct gaccaaacat tgccttattt gaagcaattc
     2161 taagagtcgc agatatcgct aactcacatc cgccaccgaa agcaaaacca ttcactgcgg
     2221 caagtgaagg tttccccatc gtttccaaaa gtcgaaaaac ctcttgtccc tggcaagcaa
     2281 attcataacc agtttgagcg ttacattcag ccagtcgatt aatgtcagcc ccagcgcaaa
     2341 atgctttacc atttcctgtt aacaataatg ctttgacttt gggattggat ttcgcttgaa
     2401 agaataattc atttaatgcg ctcagaacat cagtgcttaa agcattgagc ttctctggcc
     2461 tattcaaagt taaagttaag attccgttat tatctatatc ctgttcaatt aaattcatca
     2521 ataatcccct atgattactc tataaaatac tcttcatcca aacttgcttt cgctataatt
     2581 tcacgcatga tttcattagt gccttcaaga atttgatgaa ctcttaaatc tctaaatatt
     2641 ctttcaattt gataatctcg caggtagcca taacctccat gcaattgcat ggctttatca
     2701 ctaatacgaa atgcgacatc tgtagccaag cgcttgccca tagcacaata cattggcgca
     2761 ttgggatcat cattatccat tgctgctgca gccctataaa ccatcagcct tgccgcttca
     2821 taatctgtta acatatccgc aaaataaaaa cgaagggctt gcatctgagt taaaggtttt
     2881 ccaaactgtt tacgctcatg catataagat tggctcattc gcaggcaagc aagagcccct
     2941 cccaatgagc aggaagcaat attcactctc cctccgttta aggcattcag tgcgatttta
     3001 aaacccatac cttcttcacc aacacgattg gcaatgggca cacgacaatt ttcaaaatat
     3061 agcatacaag ttggctgatt tcgccaacct aattttttct ccagtttgcc aaatgtcaga
     3121 ccaggagtat tcttttcaat taacaaacaa ctgatgccat gatgagattc atcgcctgta
     3181 cgtaccatac ataaatacac atcactaaca cttccacccg atatgaatgc tttcgcacca
     3241 ttaagaacat aatattcacc ctcttttacc gcgcgagttt ttaaagaggc tgcatcagat
     3301 ccggattctg gctctgtaag acaataactg gctatcactt ccatactggt taattttggc
     3361 ccccatttct tgcgaatctc ttgactgcca tagcggtcga ccaatgaagc aaccatatta
     3421 tggatggaga gatatgcact tgttggaata cagcccgtag cgagttgttc aaagatgagt
     3481 gctgcatcca gacgagagag ctgagcacct cctatgtctt cgcggactac cattcccgcc
     3541 atacctaatt gtgccgcttc tcttaaagtt tcgattggaa agtaaccttg ttcatcccag
     3601 aaatctgcca ttggagccag tttgttacga gcaaactcgg aggccatttc tcgaaatgcc
     3661 aaatgttcat cagtgaaatt aaaatgcata gaccatcctt agatttattg attttagtca
     3721 ttggatttat ttatgacgct ttcttaaaaa atgatattat tgcgaatttt tagcgcccag
     3781 gaaagataat aagaataatt acatgggaac tttagcgcaa ccatctttta taaatccaaa
     3841 cgcattagct gttttaaaaa aatattttgg atttgattcc ttcagacctc ctcaggaaaa
     3901 aataattgag gatgtcattt ctggggaaga tgtactggtt ttgatgccta ctggaggggg
     3961 aaaatcgctt tgctatcaaa ttccagccat tgtccgccct ggtgttggca ttgtagtatc
     4021 tcctttaatc gcgttaatgg aagatcaagt aaccgctctc aaactgcagg gtattcgtgc
     4081 ggcttactac aactcatcct taaccgccga agaggctaga aatgtattaa atcaattaca
     4141 tcataatgaa ttggatttac tctatattgc tccagaacgt cttattagta cctcgtttct
     4201 ggatcggctg tcagaatgta ctatctcttt atttgctatc gatgaagcac actgtatttc
     4261 acaatgggga catgattttc gacctgaata cgcctgttta ggtttactga aaactaactt
     4321 tcctgacatt cctatgattg ctctgacagc tactgccgat aagcaaacac gtgaggatat
     4381 tgttaccaag ttgaattatc aacccaaaaa atacgttgtt tcattcaatc gaccgaatat
     4441 ccattacaaa gtagttccta aaacaaatgc agtgaagcaa ttaaatctgt ttttacaatc
     4501 tgatacccag caatctggaa ttatttattg tggaacccgc catacagtcg aacatcttac
     4561 agcgaaatta caagaacttg gatttaaagc acgcgcttat catgcaggcc tgcctcatgc
     4621 ggtgcggcgt gaggtgcaaa accttttcag atacgatcgc attgatattg tggtagctac
     4681 tattgctttt ggaatgggta ttgacaagcc taatgttcgc tttgttgttc accatgattt
     4741 acccaaaagc atcgaaggtt actaccagga aacaggtcgt gcaggacgtg acggattacc
     4801 ggccaaggcc cttcttcttt atgatgctgc ggatagtgcc cgtttgcggt cctggatcat
     4861 gaacattcct cttgaggagc aaagacatat agaaaccaat aaattaaatc atatgcttgc
     4921 ttttgctgaa gcgtcgcatt gtcgtcgtca aattttatta cgctattttg atgaaccttc
     4981 tcatacagaa tgtaaaaact gtgatgtctg cgataaccca ccgcaaacag ccgatgcaac
     5041 agaagatgcg caaaaatttt tatcgtgtat ttatcgatta aaacaaagct atggtttaac
     5101 tcatactatt gatgtcctga gaggtagttt atctgaaaaa atcaagcagt ctgggcatga
     5161 gcatttaagt acctttggaa ttgggaagga gaaatctgcc aattattgga agcatcttgc
     5221 ctggcaatta attcataaag attattgttt acaggacatg aatcatttta atgtgttacg
     5281 cttgacccca aaagcgattc ccctcctaaa aggtgaagag agtatttcat taaccattcc
     5341 taacaatgac ctgaatagca gtaaaaagaa aggcaagcag aatccttcct ttaaacctga
     5401 aagtaaccca ttatttgaaa agcttcgtgc gttaagacgt cagttagccg atgaagaaaa
     5461 caaaccacca tttatgatat tcagtgatgc cacattacat gaaatggccc aatcaaaacc
     5521 caaaaacagt aagcaactac tgaatgtatc tggggttggg caacataaat tagctcgtta
     5581 tggccattat tttctcgaag ccttaaacga attttcagaa gtcgagtgag attccgttat
     5641 gcgtaaatta ctaagacctt tttacgaacg tgataccgtt ctggttgcaa aagaactttt
     5701 aggtaaatat ttagttcatc atgatggatt ggaagagaaa attgggcgca ttgtagaagt
     5761 cgaagcctac cttgggcaac atgatttggc ttgtcattca tcaaaaggat tgactaaaag
     5821 aactaaagtt atgtttgggc cagcaggcta tgcgtatgtt tatctaattt atggcatgta
     5881 ttattgcatg aacgtagtca ccgaaaagga ggggataggt tcagcagtat taatacgtgc
     5941 cctggagcct atcaaaaaca ttcaagacag aactcaagga ccagggttat tatccaaagc
     6001 tatgcgcatt gactccaaac tgaatcatcg tgatttatta agcaatgatt tttatattgc
     6061 tgagccaaac agtccaactg attttaccat tatagaaaaa cccagaattg gggttcatta
     6121 tgctaaagaa tgggctaatg agttattaag attttatatt aaagacaatc cttatatttc
     6181 gaaaacctga ttaggcctcg tgcataaact gtctctttca ttttattgcc gcattgcaaa
     6241 gatttctgtg atgctcaggg tatgtaaaaa gcgtattatc gtattaatga gcccattatt
     6301 attacatccc tttcttcaag catgtcctgt caatccccgg atccaggctt tgtgcctaat
     6361 ccggggtaat ttcttgaaat aactctactc taacccaatt gccttgggcg ggcagatata
     6421 ggcattaccc agattagtca cattggtttg ttctgaaccg ccgctcataa aaccaccgtg
     6481 acgatcaaat gaaccgctca tggaaccagt tacaccggct cggttcgtca ccaattgaat
     6541 gtaatttgcc ccttttttgc ttgctttatt cttcagatca ttcattgctc cctcttccaa
     6601 attgcgattg gaagtaaagc ctccggtaaa gaaattaccc tgattgccta cgacctggcc
     6661 cacgtatttg caacctttag gtgccggatt cgggcttgca atgacacgtt cggactgagg
     6721 atctgctgga atagctgcgc atccggtaat catcaaagta ataacagacg cagatagtag
     6781 ttttttcata attacacctt tttgttatca acaaaacaat gaaatgttaa caactcctgt
     6841 ttgattaaac attacaaccc acttttaaat gcacttgata atatttcact ttattatcat
     6901 caatgaaacc tcttgtttct actacttcaa accaatccaa tttcccatgc gttttagcca
     6961 cttgctttat tgcattatca attgcttttt caattccttc ctgcgaagtc ccaattaact
     7021 ccacgatttg gtaaacttta tctgtcactc ttctttcccc ctctattaat ttgtttctca
     7081 gcttttgcta ttgccttgcg aatagccaat gcttcttcgg actgcgctgg cgccaactgg
     7141 agtagccgtt gccagtaatt aatcgcagct tcataatcat gacttaagta agcatgcatc
     7201 gcaagcatgg ctaaagcatc cggttgcctg gggttatttt ttaataaggt atgaaaaatt
     7261 tctaaaatat caggattgaa ctgttgatta ctcaattgcc ataaactgtg ggcataattg
     7321 actgcaaatt gctcgtcttc aggtttaagc tggtgggctt ttgcaaaagc gagggaagca
     7381 ttttggttat ccttttgact actgtataat ctgcctaaca aataccaacc cttggcactt
     7441 tcaggctgcg catccaactt ggccttcagc ttgtcgatta attcctgagg gcttttaatc
     7501 gattttagca tcgattgcgc tagctcctga gcctcatttc gatgtagata ttcttgccac
     7561 cgagcaaaat ctccccaaaa atagtaccct gtaaaaacca ttataaaaat aatgggggcg
     7621 agcaacaaac ttaccaaacg attgcgccgc aaaggatagg taataagagt gcatgcaaaa
     7681 acggctaaaa caaccaataa aataactaac caccattcat tcatgagaat ttcgcttaaa
     7741 acatgtccgc caaaaaataa taaagccaat caataaaaac aggaatgggc caaaccacaa
     7801 taaaagagtc acgctcttaa caggcggttt aaatagtata aaatccccat aacgtgcagt
     7861 tagataatcc attatttcac tatcgctttt gccctctttc actaattcat aaacctgaaa
     7921 acgtaaatcc ttcgctaatt gagcatttga atctgccaaa tcctgatttt ggcataccaa
     7981 acaacgcaaa tcttttaaaa gatggttaaa ttgtgcttct tttcttgctg agtccaatgg
     8041 atacatgcta tttgcaaacg caacggatac taatagcagc aataaaatca ccttaaaaca
     8101 gaagtggaat actctcatta tgatttctcc aaattcctca ttaaaggtaa aatatcagtt
     8161 aaccaggttt tctgattcat tattccgaca tgcctgtagc ggactattcc tttcttatca
     8221 atgacaaaag tttcaggcgc cccgtataca cctaagtcaa tagcgacttt accttctgaa
     8281 tcctggccta tgagtcgata agggttcccc cattgtgcaa gccatgctct tgcgtgttct
     8341 ggattgtctt tataatttaa accatagata ggtacgcctt ctcttgcgag ttgcaacata
     8401 aatacttgct cttcaacgca agcctcacac caacttgccc agacattaag caaaacaacc
     8461 tgattatgaa agttcattac actaaaagta gaatcgggat tttgtaactc tggtaaggca
     8521 aagtctggta atggctttcc aattcgagct gaaggcaaat gatgcggatc caaagataaa
     8581 cctcgccaga ggaaaaaaca taaaagacaa aaaatagcga taggaataac acgccatcct
     8641 atttttttca tatcttgatc tcctgccctg acattaccgt tacttgcctt tttctgtaat
     8701 accttctatc tgttaatgcc aaaacgcctc cggctaaaat cataaatccg ccagcccaaa
     8761 tccagcggac caacggttta taataaatac gtaccgacca ggcatcctca gacagtggct
     8821 cccccaaagc aatgtaaata tctcggaaag gagtcacatc aatcgcagac tcag
//
LOCUS       DACTJE010000135         8873 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.135, whole genome shotgun sequence.
ACCESSION   DACTJE010000135 DACTJE010000000
VERSION     DACTJE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8873
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.135"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            109..2415
                     /locus_tag="I9760_002316"
     CDS             109..2415
                     /locus_tag="I9760_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional SulP family inorganic anion
                     transporter/carbonic anhydrase"
                     /protein_id="HAT5044076.1"
                     /translation="MIDRNIVNLRLFRIYSKRYLKFDFVAAIVVFLVAIPLCLGIALA
                     SGAPLFSGILSGIIGGIVVGIFSGSQVSVSGPAAGMAAVVLAAISQLGDFNTFLLALT
                     IAGLLQMIIGALRAGFVADYVPSNVVQGLLCSIGILLIIKQLPLAFTLSSDFDELKTH
                     LLETTEGFTVSPLLALSQHINEGALIITTLSLAILIYFDITKNKILKEIPAPILVVLA
                     GILLNELFIWTNSSLAQNSPQLVNIPDTNGFFQFFSHLEYPDWSAWTNPKVYLYALVI
                     CIVASLETLLNLKASERLDKKRRHSPTNQELVAQGLGNITSGLVGGIPVTSVIVRTSI
                     NIHAGSKTKFSAVLHGFFILFAVMLIPGALNKIPLSSLAAILIYTGYKLNKPAIYINI
                     FSQGSDRFIPFIVTVISIIAFNLLAGILIGLAISLFYILKSNSQARIDIIKEIYPNGS
                     TYRLMLPQQMTFLNKAALVAELDTLPRRSQLIIDARHSQYIDKEILDLFNELKEELAG
                     SKQISVNFTGFQEHYKIHNYIDFITVTTYDVQTNLTPAQVLNILLEGNQRFLSDNRIH
                     RSNQIDIKYTAKTQHPIAVVLACIDSRVPVETIFDMSFGDLFCVRIAGNVINDDILAS
                     IEYACNVVGAKLIMVLGHTRCGAIQSACDGIEKGHITQLLSKIKPAVNAEKETTTERN
                     GKNQTFVNHVTELNVANTLQNIYKKSDILRTMIDSNEIGMVGAIYDVSSGKVSCKTYT
                     EELSTLDGDENKLLAQKFETILLEAKAS"
     gene            2502..4214
                     /gene="lem3"
                     /locus_tag="I9760_002317"
     CDS             2502..4214
                     /gene="lem3"
                     /locus_tag="I9760_002317"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector phosphocholine hydrolase Lem3"
                     /protein_id="HAT5044077.1"
                     /translation="MKLHYIINENKIVFTSCNMRDKIITGKKIIFSQSVAKDQTKDLS
                     NFLAERFYSVNQSHNHAIIIGSSLSHQENDIEHDTILDTSGVLVTTDTNGIVNGARVA
                     TTDGLGGGNGDEEEDDEIYKVSHFSCENFLNCDQNIDTTLNLITQPKASEKKQTAPKT
                     LQHTEASMAAFIYQNHPGKGYIGEFANIGDGLIIILDKRFKIKHMVSACHIYRGFGTW
                     TPPSLQALATTANKDALLVRQTLKLAEGDIIISMTDGIWGELKTSLIAQTNDRRDIGV
                     DKEYFKTLFDELTDSPYPSSFDIARIITSSAMSRSLERRKTLIKLINEIEQQHFHEKS
                     VKTINEVLEYFLKTGHVETAQTLKAILFEDGLSDGITYFENIEIPLEMVMHDLKSRTV
                     GDCSTINVTRIPYHLDELIRSFINYPEKHQILAPLFKARIKSEADLEEAFHRLSLETV
                     QAEIECPISEAHFERAFKKETLDKTQAVLTHYFRISTGLDSKKNYQERLNDLSAYFSK
                     ESSLEKNDIKLLLSMLDSEIKPKTGVFQTLFGENQNKLYKAFHKKIELQLLDGEIENK
                     NELK"
     gene            complement(4221..7070)
                     /gene="ankN"
                     /locus_tag="I9760_002318"
     CDS             complement(4221..7070)
                     /gene="ankN"
                     /locus_tag="I9760_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkX"
                     /protein_id="HAT5044078.1"
                     /translation="MVRTMPNLPGLYFFQAYPREEIWRLFVDGRFWSKENGWRGYESR
                     EPGCLNAALESLCSIALQVEKSGEEFELSVDLIKKIHKKCGKKVEELQEKNPGELRTN
                     EPVSFGIPAGRASIKGIEEFLSLVFLTEGEAAFGPGKAGPFGPCFDKNYFKNLSPEQI
                     PNLAKQIYFDMCKYGHSNTNHFYLAVMKNVDVYLEKITQSFNQEIKIAETLDEKLKII
                     VKHIRMYEVLHPFRDANGRTFVNNLLNILLMQQGLPPATFYEPNVFDLYSAEELVVVV
                     KEAIFNTVEIIEQSKKKTPITLYGYHSSLEEQTKFRDMLDSPSYEKIKHMDLSDLNPE
                     KLHRKTQKCLSSLNEQYPLHRGAIYLSDPGEIKLLLSNRDESQINQRIEQGAPPLYVG
                     KTPAHLAVISGNMAMIDELIAKKADLSLQDYDGKTALHYAAECGNMQIMGKILKVVLS
                     QEDAIKVLNIKDNHGKTAFHYAAEFGTPEIISALTTTAVIQINEPDNSGSSAITLAYK
                     NHKLKIFDELLNSGADISDELLEAIWARKDKETLGKIIAKNEKILLNKEAFRIAISLG
                     SVSLVKKFLRAGVDIDIPLTKEQATPLMLSVNSGHLKLVSYLLKKGANKSTTDTSGNS
                     ILHYVFYSKAENREALVNIITENDKKLINQPNANGNPPLYNAVVVNDLKMATILLEKG
                     ARVDFEDRLGNNILHSVMRRCDLPIILDIVKKDSTLLHKRNSERRNPFHHALHEMPTF
                     PSSKETEEIHFMNLSDFLLKEGVDLNKKDIKGKTILDIALSKQYFHLCVKLMKAGAHT
                     NISSSSKFLKNSDANSILERPFKFKNDLKKELDENPLIAMAQINDLYVQIKNNRIRTP
                     TGYAPKEGVSFFKGKSNDAKAHDEVLSVLKELYDSKLTEMLGNLPGEGLEEIKRSQKF
                     FDGELKLLIKNQDISRKVDKKSIQEAVGTSLKLKW"
     gene            7232..>8873
                     /locus_tag="I9760_002319"
     CDS             7232..>8873
                     /locus_tag="I9760_002319"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAT5044079.1"
                     /translation="MRASQWFLVTQKETPNDAEIASHQLMLRSGMIRKLGSGLYTWMP
                     LGLRVLRKVENIVREEMNKTHAMELLMPSVQPAELWQETGRWETFGGQLLTMKDSNQR
                     EYCFGPTHEEVITDIMRNELQSYKQLPVNFYQIQTKFRDEIRPRFGVMRAREFIMKDA
                     YSFHLSLESLQETYKDMYQAYCRIFDRMGLKYRAVEADTGAIGGSASHEFQVLAESGE
                     DLIFYSDASDYAANIEQATSLKPPKANQACNETITLVDTPNQKTIDEVASFLGIASNQ
                     TIKTLIVKGKEHPMVALVLRGDDELNEVKATKHPLVHSPLSFIDEELILKTLKTPLGS
                     IGPIQLNIPVIVDHHALAMPSFVCGANQADKHFINAAWERDAKYDDAYDLRNVKEGDQ
                     SPDGRGTLHCCRGIEVGHVFQLGDKYAKAMNASVINEQGQLQTMIMGCYGLGITRVVA
                     AAIEQHHDEHGIIWPQALAPFQVNIIPLNGARSQTVKEQAESLYQQLKSHGIDVLLDD
                     RNERAGVLFADNDLIGIPHRLVVSERNLEQGCIEYKSRT"
BASE COUNT         2781 a         1718 c         1612 g         2762 t
ORIGIN      
        1 tgacaaatgg aattaaatat ataaaaatag gctttcaaat tatcacatga cctgtggctt
       61 tgtttcatta acccgatact gaattcaaaa taaaggaact tttagattat gattgataga
      121 aatatagtta acctgcgtct atttcgtatc tactctaaac gttatttaaa atttgatttt
      181 gttgctgcaa ttgtcgtttt cctcgtggcg attcctttat gcttaggtat tgccctggct
      241 tctggcgcac ctcttttttc tggtatatta agtggaatca ttggcggtat tgttgtaggc
      301 atatttagcg gctcacaggt tagtgtcagc ggacctgctg cgggtatggc agctgttgta
      361 ttagcggcaa tctctcaact aggtgatttt aataccttct tattagctct tacgattgca
      421 ggccttttac aaatgataat aggtgcatta agagcaggat ttgttgcaga ttatgttcct
      481 tccaacgtag ttcaaggctt actgtgttca atcgggatct tgctaattat taagcaatta
      541 ccacttgcat tcactctctc atcagatttt gatgagctta aaacacattt attggaaaca
      601 acagaagggt ttacagtaag ccctttgtta gctttgtcgc agcacattaa tgaaggcgca
      661 ctcatcatta ctactctttc attggctatt ttaatctatt ttgatataac caaaaataaa
      721 attctgaagg aaatcccagc ccctatcctt gtagttctgg caggaatact gttaaatgag
      781 cttttcatct ggacaaactc cagcctggca caaaactcgc ctcaactggt taatatccca
      841 gataccaatg gttttttcca atttttcagc catcttgagt accctgactg gtccgcctgg
      901 actaatccta aagtctatct ctatgcgcta gtcatatgta tcgttgcttc tttggaaacg
      961 ttgcttaatc tcaaagcgtc agaacgattg gataaaaaaa gaaggcacag cccaactaac
     1021 caggaattag ttgctcaagg cttgggtaat ataacatcag gcttggttgg cggtattcct
     1081 gttacatcag tcattgtcag aacatccatt aatatccatg ctggttcaaa aaccaaattt
     1141 tcagccgtac tccatggttt tttcatcttg tttgctgtca tgttaatacc tggtgcattg
     1201 aataaaattc ctttgtcttc tctcgctgca attttaattt acactggcta taaactaaat
     1261 aaaccagcca tctatatcaa tatattttca caaggcagcg atcgattcat cccttttata
     1321 gtgaccgtga tcagtattat tgccttcaat cttcttgcag gtatattaat aggtctggct
     1381 atcagtctct tttatatatt aaagtccaat agccaagctc gtattgatat cattaaggaa
     1441 atttatccaa atggttcaac ataccgtttg atgcttcccc aacaaatgac ttttcttaac
     1501 aaagctgcgt tggtcgctga attggataca ttgcccagac gttctcaact gattatcgat
     1561 gcgcgtcatt ctcaatacat tgataaagaa attctggact tatttaatga attgaaagaa
     1621 gagttggcag gcagtaaaca aatttctgta aatttcaccg gatttcagga acactataaa
     1681 attcataact acatcgattt cattacggtt acaacctatg atgttcaaac taacctgacg
     1741 cccgcacaag tattaaacat actgcttgag ggaaatcagc gctttttaag cgataaccgg
     1801 attcatcgat caaaccaaat cgatataaaa tacactgcaa aaacacaaca tccaattgct
     1861 gtggttttag cctgtatcga ttccagagtt cctgtagaaa ccatctttga tatgagtttc
     1921 ggtgatcttt tttgtgtccg catagcaggc aatgtgatta atgacgatat tttggccagc
     1981 atagaatacg cctgtaacgt agttggtgca aagcttatca tggttctggg acatacccga
     2041 tgcggcgcca ttcaatctgc ctgtgatggt attgaaaaag gtcatatcac ccaattgctc
     2101 tctaaaatta agcctgcagt taacgcagaa aaagaaacta caacagaacg taacggaaaa
     2161 aatcaaactt ttgtaaatca tgtcactgaa ttaaatgttg cgaatacctt acagaacatt
     2221 tataaaaaaa gtgatatttt acggactatg atagacagta atgagatagg gatggttggt
     2281 gctatttatg atgtaagcag cggaaaagtg agctgtaaaa catataccga agagttatcc
     2341 actttggatg gagacgaaaa taagctcctg gctcaaaaat ttgaaaccat tcttttagaa
     2401 gccaaagcaa gttaaacttt tttgaaattg taaaaatacc ggaaagaaat aaatttctca
     2461 agccaggcat aagattttct acaagccatt aagcctgacg tatgaagtta cactatatta
     2521 ttaatgagaa taagattgtt tttacgagtt gcaatatgag agataaaatt atcaccggga
     2581 aaaaaattat cttttcacaa tccgttgcaa aagaccaaac aaaggattta tcaaactttt
     2641 tagcagaacg gttttactct gtaaatcaaa gtcataatca tgccattatt atcggttcca
     2701 gtctttctca ccaagagaat gatatcgaac atgatacaat actcgataca tccggcgtgt
     2761 tagtgactac tgatacaaat ggcattgtta acggggcaag agttgcaact actgacggtc
     2821 tgggtggtgg aaatggcgat gaggaagaag atgacgagat atacaaagta tctcatttca
     2881 gttgcgagaa ttttttgaat tgcgaccaaa atatagatac gactctaaac ttgattaccc
     2941 aaccaaaggc ctctgaaaaa aaacaaacag caccgaaaac attgcaacat acagaagctt
     3001 cgatggccgc ttttatttat caaaatcatc cgggcaaagg atacataggt gaatttgcaa
     3061 atattggtga tggactaatt attatcctgg ataaacgttt taagataaag catatggtat
     3121 ccgcatgcca tatatatcgt ggatttggta cctggactcc accatcactg caagccctgg
     3181 caacaactgc aaataaagac gccctgcttg tcagacaaac acttaaactt gcagaaggcg
     3241 acattataat atcaatgaca gatgggattt ggggggagct gaaaaccagt ttgattgctc
     3301 aaaccaatga ccgtcgagat ataggagtgg ataaagaata tttcaaaacg ctatttgacg
     3361 aattgacaga ctccccctac ccttcttcat ttgatattgc tcgcatcatt accagtagcg
     3421 caatgtctcg aagtttggaa cgcagaaaaa cattaataaa attaattaat gaaatagaac
     3481 aacaacactt ccatgaaaaa tctgtcaaaa ccattaatga agtcctggaa tattttctta
     3541 agacaggcca tgtggaaaca gcacagacat taaaagcaat tctctttgaa gacggtttga
     3601 gtgatggaat aacttatttt gaaaatattg aaatcccgct ggagatggta atgcacgatt
     3661 taaaaagtcg taccgtcggt gattgttcaa ccatcaatgt aacgcgcatt ccctatcatt
     3721 tagatgagct catacgaagt tttatcaatt accctgagaa acatcaaatc ctggcgccac
     3781 tatttaaagc aaggataaaa tctgaggctg atttagaaga agcatttcat cgcctgtcac
     3841 ttgaaacggt acaagctgaa atcgagtgcc caatttctga agctcatttt gaacgtgctt
     3901 ttaaaaaaga aacccttgat aaaactcagg ctgtcttaac tcactatttt cgtatttcga
     3961 ctggtttaga tagtaaaaaa aattatcagg aacgattaaa tgatctgagt gcatacttct
     4021 ccaaggaatc ctctctggaa aaaaatgata ttaaattgct attatccatg ctagatagtg
     4081 aaataaaacc taaaaccggg gttttccaaa cattgtttgg ggaaaaccaa aataaattat
     4141 acaaggcatt tcacaaaaaa atcgaattgc aattattgga tggtgaaata gaaaataaaa
     4201 atgaattgaa atagcagttc ttaccatttt aatttcaagg atgtaccaac tgcttcctga
     4261 atagactttt tatcgacttt cctcgaaatg tcctggtttt taattaataa ttttagttcg
     4321 ccgtcaaaaa atttctggct tcttttgatt tcttctaatc cttcgccagg aagattaccg
     4381 agcatctctg tgagtttact atcgtagagt tcctttaaaa cagataaaac ctcatcatgt
     4441 gctttggcat cgtttgattt tcctttaaaa aaggatactc cttctttcgg ggcataacct
     4501 gtaggagtcc taatcctgtt atttttgatt tggacataca aatcattaat ttgtgccata
     4561 gcaattagag gattttcatc gagctctttc tttaaatcgt tcttaaattt aaaaggacgt
     4621 tccaaaatag aattggcatc ggagttttta agaaattttg atgaagaaga tatattggta
     4681 tgggcacctg ctttcatcag tttgacacat aaatgaaaat actgcttgga taatgcaata
     4741 tctaatattg tttttccttt aatgtctttt ttatttaaat cgactccttc ttttaaaagg
     4801 aaatcactta aattcatgaa gtgtatttct tcagtttctt ttgaggatgg aaatgtaggc
     4861 atctcatgca atgcatgatg aaaaggattt cttctttcag agtttctttt atgaagtaaa
     4921 gtactgtctt tcttgacaat atccaatata attggcaagt cacatcgcct catgacacta
     4981 tgcaaaatat tattacccag cctgtcttcg aaatcgaccc ttgccccctt ttccaataaa
     5041 attgttgcca ttttcaaatc attaactacc acagcattat aaagaggtgg atttccatta
     5101 gcatttggct gattaattaa tttcttatcg ttctcagtta taatatttac taaagcttcc
     5161 ctgttttctg cctttgaata aaaaacataa tgcaggatag aattaccact ggtatcggtc
     5221 gtacttttat tagctccttt tttcaataaa taactcacta atttgagatg accggaattt
     5281 acagataaca tcagcggtgt tgcttgctct ttggttaagg gtatatcaat atctaccccg
     5341 gcacgtaaga attttttaac cagactgaca cttcctaaag aaatagctat cctgaaggct
     5401 tctttattca aaagaatttt ctcatttttg gcaattattt tacctaatgt ttctttatcc
     5461 ttccgagccc atatcgcctc caaaagctca tcactgatat cagcaccaga atttaatagt
     5521 tcatcgaaaa ttttcaattt atgattttta tatgccaaag tgattgctga agagccactg
     5581 ttatccggtt cattaatctg aataaccgct gtggttgtca aggcagatat tatttccggg
     5641 gttccaaact cagcagcata atggaatgct gttttgccat gattatcctt gatattcagc
     5701 actttgatgg cgtcttcctg acttagcaca acttttagta tttttcccat aatttgcata
     5761 tttccacact ctgcagcata gtgaagagct gtttttccat cataatcctg aagagataaa
     5821 tccgcttttt ttgcgataag ctcatcaatc attgccatat ttcctgatat gacagccaaa
     5881 tgagcaggag ttttaccaac ataaagaggg ggtgctcctt gctctatacg ttgattaatc
     5941 tgagattcat cgcgatttga caataataat tttatctcac cgggatcact aagatagatt
     6001 gcgcctctgt gtaatggata ttgctcattt aatgatgata aacatttttg agtcttccga
     6061 tgcagttttt caggatttaa atctgacaag tccatatgtt tgattttctc atacgatggg
     6121 ctatccagca tgtcccgaaa tttggtttgc tcttccaagc ttgaatgata tccatataaa
     6181 gtaatgggag tctttttttt actttgttca ataatttcta ctgtattaaa aatcgcttcc
     6241 ttcacaacaa caactaattc ctcagcgcta tataaatcaa agacattagg ttcgtaaaac
     6301 gtcgctggcg gtaaaccttg ttgcattaaa agaatattta ataaattatt gacaaaggta
     6361 cgaccattgg catctcgaaa aggatgtaat acttcgtaca ttctaatgtg cttaacgata
     6421 attttaagtt tttcatcaag agtttcggcg atttttattt cctgattgaa agattgggta
     6481 attttttcca ggtaaacatc cacatttttc attactgcaa gataaaagtg attagtatta
     6541 ctatgcccat atttgcacat atcgaaatag atttgcttgg caagattggg tatttgttct
     6601 ggactcagat ttttaaaata gttcttatca aagcatgggc caaatggacc cgctttgcct
     6661 gggccaaatg cagcctcgcc ttctgtaaga aataccaagc tcaggaattc ttcgataccc
     6721 ttaattgagg cccggccagc tggaatacca aatgaaacag gttcatttgt tctgagttca
     6781 cctgggtttt tttcctgtaa ttcctccacc ttttttccac acttcttgtg tattttcttt
     6841 attaaatcaa cgcttaactc aaattcctct cctgatttct caacttgcaa tgcaatagaa
     6901 cataaactct ccagagcggc gttaaggcat cctggctctc tcgactcata gcctctccac
     6961 ccattttctt ttgaccagaa acgtccatca acaaataatc tccatatttc ttcccgtgga
     7021 taggcttgaa aaaaatataa tccaggtagg tttggcatag ttcttaccaa ttaaaaatat
     7081 caacttatta ataattatag gacaatgtgg tcaaatatgg tcttttgaga agagctgaga
     7141 aactaaggac ctaaactaaa catatcgcaa attgaagggt gataagtata taatatcgac
     7201 cttgattatt tatagaaatc caggaataga catgcgcgca tctcaatggt ttttggtcac
     7261 tcaaaaagaa actcccaacg atgcagaaat agcatcccat caattaatgc taagatcagg
     7321 aatgattcgt aagcttggct caggacttta tacctggatg ccacttggcc ttagagtgct
     7381 tcgtaaagtt gaaaatatag ttcgggaaga aatgaacaaa actcatgcca tggaactgct
     7441 tatgccatca gtacaacctg ctgaattatg gcaagagacg ggacgttggg aaacctttgg
     7501 tggccaatta ttaactatga aagacagtaa tcaaagagag tactgctttg ggcctaccca
     7561 tgaagaagtc attaccgata tcatgcgtaa tgaattgcaa tcgtataaac aattgccagt
     7621 caatttttat caaatacaaa ccaagtttcg cgatgaaatc agaccacggt ttggggtaat
     7681 gcgagcccgt gagtttatta tgaaagatgc ttactccttc cacctcagtc ttgaaagcct
     7741 gcaagaaact tataaggata tgtatcaagc ctattgcagg atttttgata gaatgggact
     7801 caaatatcga gcagttgaag ctgatacggg agccattggc ggttcagcgt cccatgagtt
     7861 tcaggttttg gctgaatcgg gtgaagatct gattttttat agtgacgcca gcgactatgc
     7921 tgcaaatatt gaacaagcga ccagcttaaa accacccaaa gcgaatcaag catgcaatga
     7981 aacaatcaca ttggtcgata ctccaaatca aaaaacaatt gatgaagtag ccagcttcct
     8041 tggtattgcc agtaatcaaa ccatcaaaac cttgattgtc aaaggcaagg aacatcctat
     8101 ggtggctctg gtcttaagag gagacgatga gctcaatgaa gtcaaagcaa ccaaacatcc
     8161 cttagttcac tctcccctgt cctttattga tgaagaactg attttaaaaa cgttaaaaac
     8221 ccctttaggc tccatagggc caatccagtt aaacatccct gtcattgttg atcatcatgc
     8281 cttagccatg ccttcctttg tctgtggcgc caatcaggca gataaacact ttatcaatgc
     8341 tgcatgggag cgcgatgcaa aatacgatga tgcctatgat ttacgcaacg ttaaagaagg
     8401 tgatcaaagc cctgatggaa gaggaacgct tcactgctgt cgtggaatag aggtaggaca
     8461 cgttttccaa ttgggtgata aatacgccaa agccatgaat gcgtctgtta tcaatgagca
     8521 aggacaatta caaaccatga tcatgggctg ctatggatta gggatcactc gtgtagtcgc
     8581 tgccgcgatt gagcagcatc atgacgaaca cggaatcatc tggcctcaag ctttagcacc
     8641 tttccaagtc aatattattc ctcttaatgg cgcgagatca caaaccgtta aagaacaggc
     8701 agaatcactt tatcagcaac tcaaatctca tggcatcgat gtattactcg atgatcgtaa
     8761 tgaaagagca ggtgtattat ttgctgataa tgacttgata gggattcctc accgattagt
     8821 ggtcagtgag agaaatctgg aacaaggctg tattgagtac aaatcccgaa ctt
//
LOCUS       DACTJE010000136         8867 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.136, whole genome shotgun sequence.
ACCESSION   DACTJE010000136 DACTJE010000000
VERSION     DACTJE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.136"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1326
                     /locus_tag="I9760_002320"
     CDS             <1..1326
                     /locus_tag="I9760_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAT5044080.1"
                     /translation="SPTTWQKYSYLQAASYADEEQLNKVVEQLSLLPPLVTSGEIKHL
                     KNEIAQAGRGNAFILQGGDCAESFNDCRSEVISNKLKIILQMSLILLYGLRKPIIRIG
                     RIAGQYAKPRSSDYETINGVTLPSYRGDIVNSPEFTTTAREPNPKLLLQAYSCSAMTL
                     NFIRALLDGGFADLHHPQRWDLGFVEHSPQKNEYQHIVDSIEDALDFLNAIDGIRSSS
                     ISKVDFYTSHEALHLHYEQALTRQLKDGKWYNLSTHLPWIGMRTAQTDSAHLEFLRGV
                     QNPIGIKIGPAATPEWLSEVLSIANPQKEEGRVLLYTRLGAKLIDRLLPPLIDTVRKS
                     KIPVTWSCDPMHGNTETTEDGTKTRHFDNILSELKQALEIHRSMGSYLGGVHFELTGD
                     NVTECIGGARGLAPHDLKTAYHSLVDPRLNYEQSLEMAIQLSHQFRNES"
     gene            complement(1475..2533)
                     /locus_tag="I9760_002321"
     CDS             complement(1475..2533)
                     /locus_tag="I9760_002321"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAT5044081.1"
                     /translation="MFDLNQSLFLRALRRQPVERTPIWIMRQAGRYLPEYRKVREHAG
                     DFLNLCKNPELACEVTLQPLRRYALDAAILFSDILTIPDAMGLGLYFAEGEGPRFTNP
                     LQDTKAIHTLKIPSIPESLSYVFDAARLIRQEMPKELPLIGFSGSPWTLACYMVEGGS
                     SRDFKRILNLIYTEKEAAHLLLNKLAVSVTAYLIEQIKAGVNAVMIFDTWGGVLTPQN
                     YKDFSLAYMHQIVQQLKKEYPDIPVILFTKNGGQWLEWMAETGCDALGVDWTCDLASA
                     RKRVGGKVALQGNLDPAVLLTTKNCIRSEVGSVLASYGYGTGHIFNLGHGITPDVPPE
                     NVAIMIEAVHEISPQYHL"
     gene            2773..3111
                     /gene="folB"
                     /locus_tag="I9760_002322"
     CDS             2773..3111
                     /gene="folB"
                     /locus_tag="I9760_002322"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HAT5044082.1"
                     /translation="MDTLNITALSVETKIGVYGWEQRINQRLLIDISIPFDFKDCKDN
                     LSNTLDYAALCQTVTQFVESTSFQLIETVAEQIAQLIKQKYPVTKVSVAVTKPHAVKN
                     AAGIQVVVNR"
     gene            complement(3151..3846)
                     /locus_tag="I9760_002323"
     CDS             complement(3151..3846)
                     /locus_tag="I9760_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HAT5044083.1"
                     /translation="MAKDYGNRRAARRNRGPHQFLVIIVTFLFGYITASFLDVQTISQ
                     WVNTQVLAQHDDEKAPTKVAQEHKQVPPKPKFEFYTLLANERGPASQASSQQNTDNHA
                     ADKTSTPIQKTANTVTAAVNNSVKSTIANPTPQTPVKVAEAKPTTMQQPNKGAYLVQV
                     ASFKARQDAEQMKGMLILKGFDVSVVPVTQAQGNWFRVVVGPYPNKALAQKAQMNLAK
                     TEHLNGMVRSVGG"
     gene            complement(3846..5606)
                     /locus_tag="I9760_002324"
     CDS             complement(3846..5606)
                     /locus_tag="I9760_002324"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAT5044084.1"
                     /translation="MKAIIEYLLKQALINLQQSGEMPIDLEIEIKVENAKDPAHGDYA
                     TNLALVLAKPCRQAPKVLAERLVAVIPVDPSVEKIEIAGAGFINFFMRSTARSLIISE
                     ILNKGKEFGRGNLGQSQKVLIEFVSANPTGPLHVGHGRGAAFGATLGNVLKAAGYDVT
                     LEYYVNDAGRQMNILAVSVWLRYLELAGEPIVFPANGYKGQYVYEIAQEIWSEQGNQF
                     VHPWISVVENLPADEPEGGDKETYIDAIIARAQSLLGKDGFANFHQHALKTVLDDIKD
                     DLQAFGVRFDSWFSEQSLFEDGSIEKGIQALKDRGHTYEREGALWFRATDFGDEKDRV
                     LVRANGQTTYFASDVAYHWNKYDRGFDRVIDIFGADHHGYVTRIKTAVKALGHDESAL
                     DVILVQFAILYRGGDRVQMSTRSGSFVTLRELREEVGNDAARYFYVARKPEQHMDFDL
                     DLAKSESSDNPVYYIQYAHARICSVLRQLKERGLKWDKDMGLKNLDLLEQQHEATLIS
                     LIARYPEVIQSAAASCEPHQLAYYLRELANGLHSYYNAIQLLCEQEQLRCARLCLLES
                     VRQVLNNGLAILGVSAPESM"
     gene            complement(5719..6777)
                     /locus_tag="I9760_002325"
     CDS             complement(5719..6777)
                     /locus_tag="I9760_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAT5044085.1"
                     /translation="MNRIITFTAGLILVWITGYLLIAGRGLLIPIVISIFVWHLLNTI
                     NSGVQKIPFIGTHLPSWIRMILSLFVVVCLVMVLINIITNNVSDVIAASPRYQENLLH
                     IFNSLDQRFHVKGIFDFDSLIQGLSIQSMLVNIYGVFTTITSSAVLISLYVVFLFVEQ
                     HYFTQKMDAFFPQAEHRKLVKNIISHITKDTQTYLGIKTLLSLLTATLSWFIMKWVGL
                     DFAEFWALLIFFLNYIPNIGAIVATAFPAALALIQFESWWPFLEVTSGIVAIQFFVGN
                     FLEPRLLGKSLNLSPLVILFALALWGAIWGVLGMFLSVPITVMMMIIFAHFDVTRPIA
                     ILLSQDGYIHKSYESLEN"
     gene            6924..>8867
                     /gene="recG"
                     /locus_tag="I9760_002326"
     CDS             6924..>8867
                     /gene="recG"
                     /locus_tag="I9760_002326"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAT5044086.1"
                     /translation="MLSNSCEYLTGVGPTIAAKLAKCGIFTIQDLLFHLPYKYQDRTR
                     ITPIQDLRSNEWCVIAGHVYKTEIKYGKRMMLNCYVEDKTGVVKLRFFHFNKQQIQAL
                     NNSTMIRAFGEVRGFNNHLEMIHPEYQLIDQESDFHVEETLTPIYPSTQGLTQTRLRQ
                     LVKIALEQSEHELHQLEWMSEKQLQENNFYDLGEAIKLLHNPPPDISLSNLEAGEHPA
                     LKRLIFDELLAQQLSMQFARQSRSKLQAPAILFDNAIHKRFIESLPFSLTNAQQRVFK
                     EISFDLTQSKPMLRLLQGDVGAGKTIIAALAALQAISQGFQVAFMAPTDLLSEQHTNS
                     LSKWLEPIGINVLRLSGKMKTTERKNALAALQDNSCQLIVGTHALFQEQVEFARLGLV
                     IIDEQHRFGVEQRLLLQQKGQLNQLIPHQLLMTATPIPRTLSMSHFAHLDISVIDELP
                     PGRMPITTAVLNQDKRELIIERLQAAIANGKQAYWVCTLIEESEKLQCMAATDTSKKL
                     QEQLSFARVGLVHGRMKALEKEATMAAFKQGEIDLLVATTVIEVGVDVPNASLMIIEN
                     AERLGLSQLHQLRGRVGRGNNQSHCLLLYQSPLSQQGAERLKIMRSTTDGFLISEKDL
                     ELRGSGEILGTRQTGFRQFKIANL"
BASE COUNT         2807 a         1933 c         1625 g         2502 t
ORIGIN      
        1 tcgccaacta catggcagaa atattcgtat ttgcaggcag cttcatacgc tgatgaagag
       61 caattaaata aagtggtaga acaattaagt ttgctgcccc cattagttac cagcggtgaa
      121 atcaaacatt taaaaaatga aattgcccag gctggccggg gtaatgcttt tattctgcag
      181 ggtggtgatt gtgctgaatc ttttaatgac tgtcgttcag aagttataag taacaaatta
      241 aaaatcattc ttcaaatgag cctaatctta ttatatggct tgcgtaaacc aattattcgt
      301 atcggaagaa tagctgggca atatgcaaaa ccacgctcct cagattacga aaccataaat
      361 ggagttactt tacccagtta tcgtggagat atcgttaatt cacctgaatt tactaccact
      421 gccagggagc ctaatcctaa attattactt caggcataca gttgttctgc catgacttta
      481 aattttatcc gagcgctgct ggatggcggt tttgctgacc tgcatcatcc tcaacgctgg
      541 gatttaggtt ttgttgaaca ctcccctcag aagaacgaat accaacatat tgtagactct
      601 attgaagacg ccctggattt tttgaatgcc atagatggaa tacgttcgag tagcataagt
      661 aaagttgatt tttacacctc tcatgaagcg ttgcatttac attatgagca agcattaaca
      721 agacagttga aagatggaaa atggtacaat ctttcaactc atttaccctg gattggaatg
      781 cgtacggcac aaacggacag tgcacatctt gagtttctac gaggggtaca aaatccaata
      841 ggcattaaga taggcccagc agctacacct gaatggttat cagaggtatt aagcatagcc
      901 aatccgcaaa aagaagaagg acgagtttta ctttatactc gcctgggagc aaaacttatc
      961 gaccggttgt tacctccact gattgacaca gtaaggaaaa gcaaaattcc agtcacgtgg
     1021 tcatgtgacc ctatgcatgg caataccgaa acaacagaag acggtactaa aacacgtcac
     1081 ttcgataaca ttttatcgga attaaaacaa gctttggaaa ttcatcgcag catgggtagc
     1141 taccttggag gtgtccattt tgagctaact ggtgacaatg taacagagtg tatcgggggc
     1201 gctcgtggat tagctcctca tgatctcaaa actgcctatc acagcctggt tgatccaaga
     1261 ttaaactatg aacaatctct ggaaatggcc atccaattaa gccatcaatt cagaaatgaa
     1321 tcttaatgaa attcatgacg tcttgaatga aggccagtat ctatgccaaa agctgattat
     1381 ctgtaactgt tcatgcaatg gcgtgtacgg ttacgattgt ctgattcttg aggcatttca
     1441 ctattttgaa acaataattt ttgcctcgca caaactacaa atgatattga ggacttatct
     1501 catgtaccgc ctcaatcatt attgcaacat tctcaggggg tacatccgga gtaatgccat
     1561 ggcccaaatt aaagatatgc cctgttccat aaccataaga tgccagtaca ctcccaacct
     1621 cacttcgtat acaatttttt gtagttagta aaacagcagg atccagattt ccttgtaaag
     1681 cgacctttcc gcctactctc ttgcgcgcgg aagccaaatc acacgtccag tcaactccta
     1741 atgcatcaca accagtttct gccatccatt ctaaccattg cccaccattc ttggtaaata
     1801 aaattacagg gatatcaggg tattcttttt tcaattgctg aacaatttga tgcatatatg
     1861 ccaaagaaaa atccttataa ttttgtggag ttaaaactcc cccccacgta tcaaagatca
     1921 taaccgcatt aacccccgcc tttatttgct caattaaata ggcagtcaca gagacagcca
     1981 atttattcaa taacaggtgc gctgcttctt tttcagtgta tattaaattt aaaattcgct
     2041 taaaatctct actgctcccg ccctcaacca tgtaacaagc taaagtccat gggctaccag
     2101 aaaatccgat caaaggtaat tctttaggca tttcctggcg gattaagcgc gcagcatcaa
     2161 agacatagga taaagattca ggaatggaag gaattttcag ggtatgaatt gccttggtat
     2221 cttgtaaagg atttgtgaaa cgaggtcctt ctccttcggc aaaatagagc cctagcccca
     2281 tagcatcagg tattgttaaa atatcggaaa ataaaatagc tgcatccaaa gcatagcgac
     2341 gcaacggttg caaggttact tcacaggcga gttccggatt cttgcaaaga tttaaaaaat
     2401 caccggcatg ctctcttact tttctatatt caggcaaata acgcccagcc tggcgcataa
     2461 tccagattgg agttctctcc acaggttgtc ttctcaaagc tcgaagaaaa agggattggt
     2521 tcaaatcaaa catcatttaa tcactatcac aataaaaagt gtttatagta tcatgttaag
     2581 gtaatcaata cagtggtata aatagcaatc gatacttaac aatataaaca ggacacacga
     2641 aaaaccccaa ggtcaaagca aacaatgttt tcatgagaaa atttagataa attaaatgga
     2701 tgattttaaa aattttaaac aaaaagattg aggatttttc gtaggtcctg ataaattatt
     2761 aatggatatc aaatggatac tttaaatatt acagctctca gtgttgaaac aaaaattggt
     2821 gtttatgggt gggagcagcg tattaatcaa cgattattaa ttgatatatc cattcccttt
     2881 gactttaaag attgcaagga taatttgtcc aatacactgg attacgctgc tttgtgccag
     2941 acagtaaccc aatttgtaga gtcaacttcg tttcaattaa ttgaaacggt agcggaacaa
     3001 attgcacaat taatcaagca aaagtaccct gttactaagg tttccgtcgc tgttacaaag
     3061 ccccatgctg taaaaaacgc tgcgggaatc caagtagttg taaacagata atctgaaagg
     3121 agcaatcttc tttgattaaa tgattaccct ttatccgcca acgctgcgca ccatgccgtt
     3181 caggtgctct gttttggcaa ggttcatttg agctttttga gccaaggcct tatttggata
     3241 agggcccacc actactctga accaatttcc ctgtgcttgg gtgactggaa ccacgctgac
     3301 atcaaaaccc ttaaggatta gcatcccttt catttgttct gcatcctgac gcgctttaaa
     3361 agatgcgact tgaaccaaat atgctccttt attaggttgt tgcatggttg ttggttttgc
     3421 ctccgctacc ttcactgggg tttgcggtgt tggattagct atcgttgatt tcactgagtt
     3481 atttacagca gcagtgacag tatttgcagt cttttgtatt ggagttgatg tcttatctgc
     3541 cgcatgattg tctgtatttt gctgactaga tgcctgagat gccggccccc tttcattcgc
     3601 taataatgta taaaactcaa atttaggctt aggaggcact tgtttatgtt cttgcgccac
     3661 tttagttggc gctttttcat catcatgttg agctaaaacc tgtgtattca cccattgact
     3721 tatcgtttgt acatcgagaa acgaagcagt tatatatcca aacaaaaagg tcacaataat
     3781 aaccaaaaac tggtgcggtc ctctgttgcg acgtgcagca cgtctatttc cataatcttt
     3841 tgccatcaca tactctcagg agctgatacg cctagtatag ccaaaccatt atttaaaacc
     3901 tgtcgaaccg actccagcaa acacaaacgg gcacatcgca attgttcttg ctcacataat
     3961 agctggatcg cattataata actgtgtaac ccattggcta gctctcttaa atagtaagca
     4021 agctgatgcg gctcgcaaga agcggcggca gattgaatga cttcgggata tcgggcaatc
     4081 agagaaatta atgtggcctc atgttgttgc tccaataaat caaggttttt aagtcccata
     4141 tccttatccc acttcaaacc cctttctttt aactgtctca atacactaca gatacgggca
     4201 tgtgcatatt gaatataata cacaggattg tcactggatt cggactttgc cagatccagg
     4261 tcaaaatcca tgtgttgttc aggttttctt gccacataaa aataacgagc cgcatcattt
     4321 cctacttctt ctcttagctc tctcaaagta acaaatgaac cactgcgagt agacatttgc
     4381 accctgtcac cacctcgata cagaatggca aactgcacca aaatgacatc caatgcactt
     4441 tcatcatgtc ctaatgcctt aacagctgtt ttgattctcg tgacataacc atgatgatca
     4501 gcaccaaaaa tatcaattac tcgatcaaaa cctctatcat atttattcca gtgataagct
     4561 acatcagaag cgaaatatgt cgtttgccca ttagcccgga ctaatactct atctttttca
     4621 tcaccaaaat cagttgccct aaaccataaa gcaccttctc tttcataagt gtggcctcta
     4681 tctttcaagg cctgaatacc tttctcaata gaaccatctt caaataacga ttgctcagaa
     4741 aaccaactgt caaaacgaac accaaacgcc tgcaaatcat cttttatatc atccaatacc
     4801 gtttttaaag catgttgatg gaaattagca aacccatcct tgcccagtaa ggattgcgct
     4861 cgcgcgatga tcgcatcgat ataagtctct ttatctccac cttcgggttc atcagcaggt
     4921 aaattttcta ctaccgaaat ccatggatgc acgaattgat taccctgctc tgaccaaatc
     4981 tcttgtgcaa tctcataaac atattgacct ttatagccat tcgccggaaa aacaattggc
     5041 tcacctgcca attccaggta gcgcagccac acactcacag cgagaatatt catttgcctg
     5101 cctgcatcat tgacataata ttccaaagtc acatcgtaac cagcggcttt taaaacatta
     5161 cccaaagttg ctccgaaagc agcccctctt ccatgtccta catgtaaagg ccctgttgga
     5221 ttggcagaaa caaattcaat caatactttt tggctctgtc ctaaattacc tcggccaaac
     5281 tccttgcctt tattcaaaat ttcagaaatg atgagcgagc gtgccgtgct gcgcatgaaa
     5341 aaattaataa atcctgcccc agctatctca attttctcta cagacggatc tacggggatt
     5401 actgcaacca atcgctcggc taatacttta ggtgcttgtc ggcatggttt agctaatacc
     5461 agagccaaat tagtcgcata atcaccatga gctggatctt tggcattctc aacttttatt
     5521 tcaatctcca aatcaattgg catttcgcct gattgttgca gattaattaa tgcctgtttc
     5581 aaaagatatt cgattatagc tttcatactt ctcatttatc cgtcaaaaat accgatatta
     5641 tgctcttttt cataccatat ggaaaggctc tcaaccccgc agcaccatca ttacaacaat
     5701 agaaacagca atagctcatt aattttcaag agattcatag gatttgtgaa tatatccatc
     5761 ttgagacagc agaattgcta tgggtcttgt cacatcaaag tgcgcaaaaa taatcatcat
     5821 cattacagtg ataggcacag acaaaaacat gcctagtacc ccccaaatgg caccccatag
     5881 cgctaaggca aacaatatca ctaaaggact taagtttagt gatttaccta atagacgagg
     5941 ctctagaaaa ttccccacga aaaactgtat agccactatc ccggaagtca cttccaaaaa
     6001 cggccaccag ctttcaaatt ggatcaaagc aagcgcagct ggaaaagctg ttgctacaat
     6061 agctcctata ttggggatat aatttaaaaa gaaaattaat aatgcccaaa actcggcaaa
     6121 atccaatcca acccatttca taataaacca actcaacgtg gcagtgagca ggctcaacaa
     6181 agttttaata cccagataag tttgagtatc ttttgtaata tgggagataa tgtttttaac
     6241 cagcttcctg tgctctgcct gagggaaaaa ggcatccatc ttttgagtaa aataatgctg
     6301 ctcgacaaac agaaacacaa catataagga aatcagtaca gcggaactgg ttattgtcgt
     6361 aaaaacccca taaatattaa caagcatact ctgaatgctt aacccttgaa ttaaactgtc
     6421 gaaatcaaaa atacccttaa catgaaatcg ttgatccagg ctgttaaata tgtgcagtaa
     6481 attttcctga tacctgggag aagcggctat cacatcactg acattattgg taatgatgtt
     6541 aataagaacc attacgaggc aaacaacaac aaacaaagat aaaatcatcc ttatccagga
     6601 tgggagatgt gtgccaatga aaggaatttt ctgaacaccg ctattaatgg tattcaacag
     6661 gtgccagaca aaaatggata taacaattgg aattaatagt cctcttcctg cgattaataa
     6721 ataacctgtt atccaaacca gtatcaaacc cgctgtgaac gttataatac gattcatcca
     6781 attacactcc atctttgatg aatagatact cgcaagcaac aagtaactta gtaatgattt
     6841 gggcaaaatt gccagaatga tgactcaagt cgcaacgaat atattttcct gttattatta
     6901 aatttttaat cttaaataat accgtgctga gtaactcttg tgaatattta actggcgtcg
     6961 gcccaaccat tgcagcaaaa cttgctaaat gtgggatttt cacgatacaa gacttgcttt
     7021 tccacctgcc ttataaatac caggacagaa ccaggataac accaattcag gatttgcgct
     7081 cgaatgaatg gtgcgttatc gcggggcatg tatacaaaac tgaaataaaa tacggcaaaa
     7141 ggatgatgtt gaattgttat gtggaagata aaacaggagt agtgaagctt cgtttttttc
     7201 atttcaacaa gcagcaaatc caggctttga ataatagcac catgattcga gcttttggcg
     7261 aagtccgcgg atttaacaac cacttggaga tgattcatcc agaatatcaa ttaattgatc
     7321 aagaatcaga tttccacgtg gaagaaactc ttacgccaat ttatccatcg actcaaggct
     7381 tgactcaaac ccgattaagg cagttagtta aaatagccct ggaacaatct gaacacgaac
     7441 ttcatcaatt ggaatggatg agtgaaaaac aattacagga aaacaatttt tatgatttgg
     7501 gcgaagccat taaattacta cataaccctc cgccagatat ctccctctcc aacctcgaag
     7561 ctggcgaaca tcctgctctc aagcgattaa tatttgatga attactggca caacagctca
     7621 gtatgcaatt tgcaaggcag tcacgcagca agctccaggc tcccgctata cttttcgata
     7681 atgcaattca taaacgtttt atcgaatcat tgccattcag tcttacaaac gcacaacaac
     7741 gggtttttaa agaaatcagc tttgatttaa ctcaaagcaa acccatgctt cgtttgctgc
     7801 aaggagatgt tggggcgggt aaaaccatta tcgcggcact agccgccctt caagccattt
     7861 cacaaggatt tcaggttgct tttatggcac caaccgattt attaagtgaa caacatacaa
     7921 atagccttag taaatggtta gaacctatcg gtattaatgt attaagactg agcggtaaaa
     7981 tgaaaaccac agaacgaaaa aatgcgcttg cagccctgca ggacaatagc tgtcagctca
     8041 ttgttggaac tcatgcctta tttcaggagc aagtcgaatt cgctcgtttg ggtttggtca
     8101 ttattgacga acaacaccga tttggagtag aacaacggct ccttctgcaa caaaaagggc
     8161 aattaaacca attaatccct catcaattat taatgacagc cacccctatt ccaagaaccc
     8221 tgtcaatgtc ccattttgcc catttggata tatcggtcat cgatgaacta ccgcctggcc
     8281 gtatgccaat tacaacagca gtattaaacc aggataagcg tgaactcatt atcgaacgtt
     8341 tgcaagcggc aatcgccaac ggcaaacaag cttactgggt ctgtaccctt atagaagaat
     8401 ctgaaaaatt acaatgcatg gcagcaacag atacctcaaa aaaactacaa gaacagctct
     8461 ctttcgcgcg tgttggtctt gttcatggga gaatgaaagc acttgaaaaa gaagctacta
     8521 tggcagcctt taaacaaggc gagattgatt tattggtggc aactactgtt attgaagttg
     8581 gtgtagatgt tcctaatgcc agtttaatga tcattgaaaa tgcagagcga cttggtttgt
     8641 cgcagttaca tcaattacga gggcgcgtgg gcagaggtaa taatcaatct cattgtctat
     8701 tactttatca atctcctcta tcacaacaag gtgctgaacg tctcaagatc atgcgttcaa
     8761 caacagacgg ttttctaatc tccgaaaagg atctggagtt aagaggctca ggagaaatat
     8821 taggaaccag acagactggc tttagacaat ttaaaatagc taactta
//
LOCUS       DACTJE010000137         8786 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.137, whole genome shotgun sequence.
ACCESSION   DACTJE010000137 DACTJE010000000
VERSION     DACTJE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8786
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.137"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            338..1183
                     /locus_tag="I9760_002327"
     CDS             338..1183
                     /locus_tag="I9760_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAT5044087.1"
                     /translation="MSKKAIAISIVIALFFITAIWFAYSFVKQEENQNWLTLYGNIDI
                     RDVSLSFRVSGRILSMDVDEGAHVKKGQVLARLDKDTFIANLEMAKAQLEQAIANQKN
                     ASRTYKRREQLVDDGAVSKALFDDAVAMKDSSSAEVSVAKANLEKAQISLNDTEIHAP
                     THGTILTRIQEPGAIVNVAEPVYTLAIDNPVWVRTYIDEPFLGRIYPGQDALVYTDSN
                     PEKPYKGHIGFISPQAEFTPKIVETTQLRTDLVYRIRVVIDQPDNGLRQGMPVTVKIH
                     LKANS"
     gene            1186..2928
                     /locus_tag="I9760_002328"
     CDS             1186..2928
                     /locus_tag="I9760_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5044088.1"
                     /translation="MNSSKPLVSINGVSKCFPGNTDPALDNVSATIFEGQITGLVGPD
                     GAGKSTLMRLICSLMLPTQGTITVDSLNTRTDSEKIHAITGYMPQKFGLYEDLTIIEN
                     LRLYAELRNLHGEKREEQFRTLLKFTALEPFQRRLAGNLSGGMKQKLGLACALMGEPK
                     LLLLDEPSVGVDPISRRELWTMVQGLLGRGMGVVWSTAYLDEAEKCDQILLLNAGKPI
                     YHGKPGDFLQKTKDRTFQICGVSLLNRRQALMNALNTPEVIDGVIQGKNIRIVISDKK
                     IKPSLDGITREPGVHFQEVESRFEDAFIDSLKTKISGRSLLAEHIAEKPHSDKPIIEA
                     RKLTKRFGSFTAVSNNEFTIKRGEIFGLLGPNGAGKSTTFKMMCGLLQPTQGQAFVMG
                     LDLKTSSSEARSRIGYMAQKFSLYSHLDALQNMRFFSGLYGLSGKRQEAQINSMIEIF
                     DLKKHLKQNSGDLPLGFKQRLALACAVMHEPDVLFLDEPTSGVDPLTRREFWTHINGM
                     VNKGVTVMVTTHFMDEAEYCDRIALIYRGKNIATGTPDDLKDKVRNSTLPNPTLEDAF
                     IELIRLDELREPVH"
     gene            2946..4067
                     /locus_tag="I9760_002329"
     CDS             2946..4067
                     /locus_tag="I9760_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT5044089.1"
                     /translation="MNIRYLKALVIKETLQIIRDPSTILIAFVLPLILLFIFGYGVNL
                     DTDQVKFGLALESYDSSTVSLSQSFSSSRFLDVSISTNRQAFHYGLVNGTIRGFIDIP
                     QRFTADSLSGNPKPALQTIVDGSYPQVAGFAENYALGALQTWISSQAYEHGQMTVKSG
                     INLEPRYWYNQELKSRNFLIPGSIVIVMTLIGTLLTSLVIAREWERGTMEAMMATPIS
                     IVDIILGKLIPYFVLGMGSMFLCTIIATWYYGVPFQGTLLALTLVSAVFLVAALGQGL
                     LISTLAKDQFVASQLALMSAFLPSFILSGFIFEINSMPKWIQLLTHLFAARYFVTSLQ
                     TLFLTGNIWSLLIRCLFAIALIGFAFFFITAHKTRKRLD"
     gene            4067..5191
                     /locus_tag="I9760_002330"
     CDS             4067..5191
                     /locus_tag="I9760_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT5044090.1"
                     /translation="MAKLSPTTRIFALVIKEILTLFKDPKGRIVLIAPPLLQLLIFSF
                     AATMEIKNISIGILNQDSGKHGYEVIQRFIGSPTFSKVFFIQKQSDIKSIIDNQEAMV
                     VMTIPQDFSRKIESNSQANIQLILDGRRSNATQIVNGYISSIIENYNQEIQKTHGAIM
                     TPPVMVTRSWFNENLLYLWFTVPSLVCILSMLISLVVTALSVARERELGTFDQLLVSP
                     LTPYEILIGKTIPAIIIGLAEGLLMWVAAVWLFDVPFYGSPLLMVYILFIFIMSTVGV
                     GLFISSMCKTQQQAILGVFIYMVPVVTLSGYASPVENMPEWLQDLTWFNPLKFTLIAV
                     IGIFLKGMSALEVFITTWPLLLVGAVTLFIAGWFFKQRME"
     gene            5200..6639
                     /locus_tag="I9760_002331"
     CDS             5200..6639
                     /locus_tag="I9760_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HAT5044091.1"
                     /translation="MDRKLALLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKIS
                     SSAPVHLTWWKAFHDPLLDRYINEAATNNLDIKAAEARIRQARAMKQISLANFYPQIN
                     GSASANSLQISKGTLEGIFANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEAS
                     KASLEEAYESRRTILLSTIAEIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYK
                     AGVSSEVDVSLAEGQLKNTESVLPNYITQIQVNAYQIAVMLGKDPQTILKDIAKIQSL
                     PKPPQKVPMGLRSDILRQRPDIRATERQLASAVAEIGVAVADFFPQFNLSVSNLAFQS
                     TSVDTLFQSSSGFWLMGPQVSLPIFHGGQIRANVESKKAVAQQAAIQYKKIILQALQE
                     AQSALVRYSQSLDTNHSLRAAIIANKRAVQFAKKRFQYGEDDYLSLLQSEAALLNAET
                     QEVNSEVSSLTALISLYKALGGGWENFEA"
     gene            complement(6964..8745)
                     /locus_tag="I9760_002332"
     CDS             complement(6964..8745)
                     /locus_tag="I9760_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAT5044092.1"
                     /translation="MKRLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEQLPINPS
                     PEFLEFVKESTPLANKLRDKWLYELARIKDWANYNKYYQPTNDINLRCFAQIAKFNLG
                     LYKEAIAGSIPIWLTGESRPQACNTLFALLLKQDNFDQKFITQRITLALDKRNVLLAR
                     YLLKQYKTPHDTEIKNLTSVYENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKL
                     WQEKKTNIMLNERQKQDFLAHLALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFA
                     LKNKNWKQVEKLINNSPNKEMPCWQYWLARSLEEQGKKAEAIKIYEPLAKNRHYYGFL
                     ASKRLKKRPSFDNEKPTTNTEILKPYQPVIEQVKNFYNSKQLLQASRLLNDFISELPK
                     EEASALVYWIATELKWHGKSVYLSNNDTLNNQLALRFPLAYKDTIKVYASKYAIPPEL
                     VYAIIRQESGFREDVTSSAGAKGLMQVMPQTASAVSKTSKIPYSDHKQLFLSQKNINI
                     GIAYLQHLTKRFSNHPVLVAAAYNAGPRQVVYWLKNHPPKEIDIWIETLPWQETRNYL
                     KNVMAFYIVYQYRLNQQPDMNRFLTPL"
BASE COUNT         2513 a         1600 c         1855 g         2818 t
ORIGIN      
        1 aggggtagag tatctgtgta aatttggctc cattccctaa cggccgtggc tctgattatt
       61 aatcagtatc aacttgctct atgtccccga tttaaaacta ttttaaaata ataaattatg
      121 gcattatgac tttctattta tgctttattg ataagaacaa aacttacgaa aaaaaaccaa
      181 ggtccaggca aaaaatgttt gtaatgaggg agtttagata gactaaacga ccgcattgaa
      241 aacattttta acaaagagat tggagttttt cataagtcct gaagaaaata taatgcgaac
      301 aaagggttag tacgccaagg aattcatgga gaactatatg agtaaaaaag cgatagcaat
      361 aagtatcgtc atcgcacttt ttttcatcac agcaatttgg tttgcgtaca gctttgttaa
      421 acaggaagaa aaccaaaatt ggcttactct ttacggaaat attgatatac gtgacgtttc
      481 attatccttt cgtgtctcgg ggcgtatcct gtctatggac gttgatgaag gtgctcatgt
      541 taagaagggg caagtactcg ccaggcttga taaagatact tttatagcca atctggaaat
      601 ggcgaaagcc caattggaac aagccattgc caatcaaaaa aatgcaagca gaacttataa
      661 aagacgtgag caattagttg atgacggggc tgtttcaaaa gcgctttttg atgatgccgt
      721 tgcgatgaaa gactcgagta gtgccgaagt ttctgtggct aaagcgaatc ttgagaaagc
      781 tcaaatatca ctgaacgata cggaaattca tgccccgact cacgggacga tactaaccag
      841 aattcaggag ccaggcgcta ttgttaatgt cgcggaacca gtttataccc tggctattga
      901 taatccggta tgggtaagga cttatattga tgaacctttt ttaggtagaa tttatcctgg
      961 acaggatgct ttggtttaca cggattcgaa tccggaaaag ccctacaaag gtcatattgg
     1021 ctttatttcc ccgcaggcag aattcacacc aaaaattgtt gaaaccactc aattgagaac
     1081 ggatcttgtc tatcgtataa gagtcgttat tgatcaaccg gataatggat taaggcaagg
     1141 aatgcctgtt actgtgaaaa tccatttaaa agcgaattca tagtaatgaa tagttcaaaa
     1201 ccactagtca gtattaatgg agtcagcaaa tgttttcctg gaaacactga tccggcatta
     1261 gataatgttt ctgcaacaat ttttgaaggg caaatcacgg gtttggttgg accggatggg
     1321 gctgggaaat caactcttat gcgtcttata tgcagcctga tgttgcctac gcaaggaaca
     1381 attacagtag acagtctcaa tacccggact gactcggaaa aaattcatgc gattactggc
     1441 tatatgccac aaaaatttgg tttgtatgaa gatttgacca ttatagaaaa cttgaggctc
     1501 tatgctgaac ttagaaatct gcatggcgag aaacgcgaag agcaatttag aacattactt
     1561 aaatttacag cactggagcc ttttcagaga cgtctggctg gtaatctctc agggggaatg
     1621 aagcaaaaat tggggctggc ttgtgcctta atgggtgagc ctaagctgtt gttacttgat
     1681 gagccgagtg ttggcgttga cccaatatcc agaagggagt tgtggactat ggtccagggg
     1741 ctgcttggaa ggggtatggg cgttgtttgg tcaactgctt atcttgatga agctgaaaag
     1801 tgtgaccaaa ttcttttact caatgccggc aaaccaatat accatggaaa accgggagat
     1861 tttttgcaaa aaaccaaaga ccggactttt caaatttgcg gtgtttccct gctaaatcgt
     1921 cgccaagctc tgatgaatgc ccttaatacc cctgaagtca ttgatggtgt catccagggt
     1981 aagaatattc gtattgtcat tagtgataaa aaaataaagc caagtttgga tggtataact
     2041 cgggaacccg gagttcattt tcaagaagtt gaatcgcgtt ttgaagacgc ttttattgac
     2101 agtctgaaaa ctaaaataag tggtcgctct ttgttagccg agcatattgc tgaaaagcct
     2161 catagtgaca agcctatcat cgaggcccga aagttaacca aacgttttgg tagttttact
     2221 gccgtttcga ataatgaatt tacaatcaaa cgcggtgaaa tctttggttt attaggccct
     2281 aatggagcag gtaaatcaac cacatttaaa atgatgtgtg gtttattgca acccacacaa
     2341 ggacaagcat ttgtaatggg actcgatcta aagacttcat cctctgaagc aagaagccgt
     2401 attgggtata tggctcagaa attttcacta tacagtcatt tggatgcctt gcagaacatg
     2461 cgcttttttt ctggtctcta tgggttatcc ggaaaaaggc aggaagcaca gatcaacagc
     2521 atgattgaaa tttttgatct taaaaagcac ttaaagcaaa attcgggtga tcttccatta
     2581 ggattcaaac aacgtctagc tctggcttgt gccgtaatgc atgagcctga tgtgttattt
     2641 cttgatgagc cgacttcggg ggttgatcca ttgacccgac gtgagttttg gacacatatt
     2701 aatggtatgg tgaataaagg tgtgacagtg atggtaacca ctcattttat ggatgaagcg
     2761 gaatattgcg atcgtatcgc tcttatttac agggggaaaa acattgccac aggaacgcct
     2821 gatgacttaa aggataaagt acgtaactct acattaccca atcccacttt agaagatgcg
     2881 tttatcgagt taatccgatt ggatgagttg cgtgagccgg tacattaaca cagggagaat
     2941 agtggttgaa tattcgctat ctaaaggctt tggtcattaa ggaaactctg caaattatcc
     3001 gtgatcccag tactatatta attgcctttg tattgccttt aattttgctt tttattttcg
     3061 gttatggtgt taatctggac actgatcaag tcaagtttgg cctggcttta gagagttatg
     3121 attcgtcgac tgtcagtctt tctcaatctt ttagcagttc ccgatttttg gatgtatcta
     3181 tttcaacaaa cagacaagcg tttcattatg gcttggttaa tggaacaatt cgtggtttta
     3241 ttgatattcc tcaacgtttc actgctgata gcctgagtgg aaatcccaaa ccagctctgc
     3301 aaaccatagt tgatggttct tatccgcaag ttgccggttt tgcagaaaat tacgcgcttg
     3361 gggctttgca aacctggatt agctcccaag cttatgagca tgggcaaatg acagttaaat
     3421 ccggcattaa ccttgagccc agatactggt acaaccagga gctaaaaagc agaaattttc
     3481 ttattcctgg ctcaatagtt attgttatga ctttgattgg aacattactc acctcactgg
     3541 tgatcgctcg tgagtgggaa agaggtacga tggaggcgat gatggcaacg ccaatttcta
     3601 ttgttgacat catcttggga aaattaatac cctattttgt gttagggatg ggttccatgt
     3661 ttttatgcac tattattgca acatggtatt atggagtacc tttccaaggt actcttttgg
     3721 cactgacatt agtttcagct gtctttctgg ttgctgcttt agggcaggga ttattaatat
     3781 ctactcttgc aaaagaccag tttgtggctt cgcaattggc tttaatgtct gcgtttttgc
     3841 cttcatttat tctttccggg tttatttttg aaatcaattc gatgccaaag tggatacaat
     3901 tactcactca tttgtttgct gcacgttatt ttgtgacaag tctgcaaacc ttgtttttaa
     3961 cgggtaacat ttggtctctt ttgattcgtt gcctttttgc gattgcactg attggatttg
     4021 cattcttttt tattaccgcg cataaaacca gaaaaaggct tgattaatgg caaaattatc
     4081 tccaacaaca agaatttttg ctcttgtcat taaagaaata ttgactttat tcaaagatcc
     4141 caagggacga attgtattga tagctcctcc actcctccag ctattgattt tttcctttgc
     4201 agctactatg gaaattaaaa atatttctat aggcattttg aaccaggatt ccggaaagca
     4261 tggttatgaa gtgattcaac gctttatagg ctcaccaact ttttcaaaag ttttttttat
     4321 tcaaaagcaa tctgatatca aatctatcat tgataaccag gaagcaatgg ttgtaatgac
     4381 catcccacaa gatttttctc gtaaaatcga atcgaattca caagccaata ttcagttaat
     4441 tttggatggt cgacgttcta atgcaaccca aattgtaaat ggttatatca gtagtattat
     4501 tgaaaactat aatcaggaaa ttcaaaaaac gcatggtgca atcatgacac ctcctgtgat
     4561 ggtcactcga agttggttta acgaaaattt gctttatctt tggtttaccg ttccttcatt
     4621 ggtttgcatc ttatccatgc tgatttctct tgtggttact gctttatcgg tagccagaga
     4681 aagagaactg ggaacctttg atcaattatt agtatcacct cttactccct atgaaattct
     4741 tataggcaag actattcctg ccattatcat tggtttggcg gaaggtctct tgatgtgggt
     4801 tgctgcggtt tggctatttg atgttccttt ttatggttct ccgttactga tggtttatat
     4861 tttgtttatt tttattatgt caacggtggg tgttggctta tttatttctt ctatgtgtaa
     4921 aacccagcag caagctattt tgggtgtatt tatctacatg gtgcctgttg tgacgctttc
     4981 cggttacgct tctccggttg aaaacatgcc ggaatggctt caggatttaa cgtggtttaa
     5041 cccattaaaa tttactttaa tagcagtaat tggcattttt ttaaaaggta tgtcggcttt
     5101 agaggttttt atcacaacat ggccacttct attggtaggg gctgttacat tatttattgc
     5161 tggctggttt tttaagcagc gtatggagta agaaacagta tggacagaaa attagcgcta
     5221 ctcttcagta tttcttgttt atttggctgt actgtcggtc ctgattatca acgcccagac
     5281 attaaaattc aggagaaatg gccaacaagc aaagtagaga aaaaaatttc cagtagtgcc
     5341 cctgttcatt taacctggtg gaaggcattt catgatccat tgcttgatcg atacattaac
     5401 gaggcagcta ctaataacct tgatatcaaa gcagcagaag ctcgtatccg ccaagcccgt
     5461 gcaatgaaac aaatttccct tgccaatttt tatccgcaga tcaatggatc agcaagtgct
     5521 aattccttgc aaatcagtaa gggtacgttg gaggggattt ttgcaaactc tccaacatat
     5581 actctaattg atagaaatca atggatatat aatttgggtt ttgatgcaca atgggagttg
     5641 gatttatttg gtaaaacaag aagaaaagtt gaagcaagca aagcaagcct ggaagaagct
     5701 tatgaatcac gtcgtaccat tttactatct acgatagccg agatcgcaag aagttatata
     5761 gaattgagag gcgcacaaca gttgtatggt attgcgcaaa aaaatgtgga attgcaaaga
     5821 aaaacgttgg ctcttgtccg ccaacgctat aaagcaggtg ttagcagcga agtcgatgtt
     5881 tctttggccg aaggccagtt aaaaaataca gaatctgttt tacccaatta tattacccaa
     5941 atacaggtaa atgcttatca aattgccgtt atgctgggta aagatccgca aacaattctc
     6001 aaagatattg cgaaaattca atctttaccc aaaccaccgc aaaaagtacc tatgggatta
     6061 cgttcggata ttctgcgcca gcgtccagac ataagagcaa cagagaggca attggcgtct
     6121 gccgtagctg aaattggtgt ggcagttgct gactttttcc cgcaatttaa tctttctgtt
     6181 tccaatcttg cttttcagag cacttctgtt gatacgttat ttcaatccag tagtggattt
     6241 tggctcatgg gtcctcaagt cagtttaccc atatttcatg gtggccagat tagagctaat
     6301 gttgagagta agaaggcggt tgctcagcaa gcagcaatac aatacaaaaa aataatcctc
     6361 caggcattgc aagaggctca aagtgctctt gtccgctata gccaatcgct cgatacaaac
     6421 catagcttgc gcgctgccat tattgctaac aagcgagctg tgcaatttgc caagaaacga
     6481 tttcaatacg gtgaggatga ttatttatca ctactgcaat cagaggctgc tcttctgaat
     6541 gccgagactc aagaagttaa ttcggaggtt tcttcattga ctgcgttgat tagtctatat
     6601 aaagcgcttg gcggaggttg ggaaaatttt gaggcttaaa catccgcgca tgctctaaaa
     6661 tcaggaattt tatcaataaa ttaatgtaat aagctatgga taatgtaggt cattatcttg
     6721 aatcgttcgg gctgaggagg catttatgcc gtctcgaagc tttgtatgga atttaatgtt
     6781 tcttgccaag gcttcgagac gatgcttact catctcctaa gcccaaacgg cccctggata
     6841 gcgatagctt cttatataat cctcgcccat ttcgttaaat tagcaaaagt cagacaggta
     6901 gttcgcaaac gtagatctgg ctatagaacg atttacgttt actaccatgc atgaagatat
     6961 ctcttacaaa ggtgttagaa acctattcat atcaggctgt tggtttaaac ggtactgata
     7021 aacaatataa aaggccatta cattttttag ataatttcgt gtttcttgcc agggaagtgt
     7081 ttcaatccag atatctattt ctttgggcgg gtgattttta agccaataga ctacttgtct
     7141 gggaccggca ttgtaagcgg cagcaacaag aaccgggtgg ttgctgaagc gcttggttaa
     7201 gtgctgtaaa taagcaatcc ctatattgat attcttttga gataaaaaca attgcttatg
     7261 atccgaatac ggtattttgc tggttttgga aacagcgctg gctgtttgtg gcatgacttg
     7321 cattagtcct ttagccccag cagaggatgt tacatcttct ctaaaaccac tttcctgacg
     7381 aataatagca tatacaagct caggcggtat ggcgtatttt gacgcatata cttttattgt
     7441 gtctttgtag gccaatggaa atcttaatgc taattgatta ttgagtgtgt cattattgct
     7501 taagtaaact gattttccat gccactttag ttcggttgct atccaataga ccagagcact
     7561 tgcttcttct ttgggtaatt cactaatgaa gtcatttaat aaccttgatg cttgcagtag
     7621 ctgtttggag ttgtaaaaat ttttaacttg ctctatgaca ggttgatagg gtttgagaat
     7681 ctctgtgttg gttgttggtt tttcattgtc aaagctgggt ctttttttca ggcgtttgct
     7741 ggctagaaaa ccataataat gtctgttttt agctagtggc tcataaattt taatcgcctc
     7801 tgcttttttc ccttgttctt ccagggagcg agccaaccaa tattgccaac aaggcatctc
     7861 tttatttggt gagttgttaa ttaatttttc aacttgtttc cagtttttat ttttcagagc
     7921 aaagcggatt tgccagtcga ctaacacatc ggtgtagtat tcaggtttga ttttggcaaa
     7981 ccattcctga gcatcggcat gatttctcat ggccttgtat aatgccaaat gtgcaagaaa
     8041 atcttgtttt tgtcgctcat ttaacatgat gttggttttt ttctcttgcc ataatttcac
     8101 tgctttatcc atgttaatgg atactaatct ttttaatcca taaagataga aataatcgtt
     8161 caatttgcca gggttcaact tcgaaatatt ggaaggattc tcataaacac tggttaagtt
     8221 ttttatttct gtatcgtgag gtgttttgta ttgttttaac aggtaacgag cgaggagaac
     8281 attgcgtttg tctagtgcaa gtgttatgcg ttgcgtaatg aatttctgat caaaattgtc
     8341 ttgttttagc aataaagcaa atagggtatt gcatgcttgt ggtctggatt ctcctgttaa
     8401 ccagattgga atagagcctg caatagcctc cttgtataat cctaaattaa atttggcaat
     8461 ttgcgcaaag catctgaggt ttatgtcgtt tgtgggttga taatacttat tataatttgc
     8521 ccaatccttt atcctggcca gttcatatag ccatttatct cgtaatttat tagcaagcgg
     8581 cgtgctttct tttacaaatt ccaaaaattc tggtgaaggg ttaataggca gctgttcata
     8641 ccatgcgaga tagcgattaa atctgtccat gtaggcttgt ccagataaag caaacaagtt
     8701 gaaagaacag agaagcccac ataaaaaaat taataatctt ttcataattc ctcttaataa
     8761 ggcttgaggg caattatttg gcactt
//
LOCUS       DACTJE010000138         8728 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.138, whole genome shotgun sequence.
ACCESSION   DACTJE010000138 DACTJE010000000
VERSION     DACTJE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.138"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            140..1387
                     /locus_tag="I9760_002333"
     CDS             140..1387
                     /locus_tag="I9760_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HAT5044093.1"
                     /translation="MNKRLFIVFILGFSSGLPMALISSTLQAWYANNGMSVLATGALS
                     LVSLPYAYRIFWGPILDRYSLFHLGKRRSWILTMQILLLLGFNLMAWFIPAQYPKLMA
                     FLALVLACFSATQDVAIDAHRAEYLPPQEHALGASLAVFGYRLALLLSGGLALVMAEK
                     LGWAFTYRFMGFAMAIGMLAIFVSKEPSTEIKEKSNFLVSFIAPVKELLSRPGIIYLL
                     IFIFCYKLGEAFTTTTSGIVMPFLIQGLGFSLDTIGYINKMLGIVSILLGGLTAGFLL
                     MRYSLYRALFFFGLLQALTNVFFVILAMSGKNIVLLAIAVFSDNFVAGMGSTALVALF
                     MRLVNKQFTGTQFSMLVALSTLPRIISGPVAATIQMHIGWVGLYQLSIVFALFFIPFL
                     VLIKEQTKERLQSDTAATPIGAA"
     gene            complement(1504..1590)
                     /locus_tag="I9760_002334"
     tRNA            complement(1504..1590)
                     /locus_tag="I9760_002334"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1554..1556),aa:Leu,seq:caa)
     gene            1773..2159
                     /locus_tag="I9760_002335"
     CDS             1773..2159
                     /locus_tag="I9760_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3757 domain-containing protein"
                     /protein_id="HAT5044094.1"
                     /translation="MKRYYSTLLVLALAHASLSAYAHTCPDPQTTSLQWGVPPSPWVE
                     NPFSPNSPQGEENTRFVRANILVAGYGQGVTCTYRNSVGDFSILWQVRTKIPARVDYS
                     WIETMGGYVCTRGLNECQFYVADPSA"
     gene            complement(2263..3606)
                     /gene="nadA"
                     /locus_tag="I9760_002336"
     CDS             complement(2263..3606)
                     /gene="nadA"
                     /locus_tag="I9760_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAT5044095.1"
                     /translation="MFKNSTTYQPEATLMAIRNDMAICQSDYPLDWYQEDFIPYAQEY
                     QALPDRKLTTVLNWMKPYLAKAQNHFGNKLLLLAHYYMGGEIVRLIEQFGGKIGDSYQ
                     LALMAANNPEKSVIVESAVHFMAESISILANENQHVYITNPKSGCTMEMLAKDFMVEP
                     AFLDLNERYGAENILPVCYMNTSGRVKAMTGAQGGAVCTSSNVKKIFQWAQKQNKKIL
                     FIPDQHMGENVAYWLGIKNLAYWPGGTAGANYSLPAQDKKTLKQFDKAELILFASQCA
                     VHTHYQPEMCDYWKSQGYTTIVHPECRNDVIKVADYSGSTAFIWDLVVHDKAGTKSYA
                     IGTENHMVENLKQYCKNLGIRVVNLAEAPQKDTEKGVGCGCATMSRNDPPHLVALLDL
                     LRQGKSMPYNEVKAGDVVNEFTGTRNRLPVNDQQWVINNAKKALEMMINITEDRV"
     gene            complement(3681..5330)
                     /gene="nadB"
                     /locus_tag="I9760_002337"
     CDS             complement(3681..5330)
                     /gene="nadB"
                     /locus_tag="I9760_002337"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAT5044096.1"
                     /translation="MSDQLSQQGQSFEFDVLVIGTGLAGLHYCLQLLAIQPKLNIALI
                     SKAESIECNSRYAQGGIAAAISPEDSLESHISDTVLAGDGLCYLPAVEFIIRQGPDII
                     KQLDQYKVQFKRENDGRFHLAKEGGHSHRRIFNCGDQTGLTLTQTLNHLARQHQQIQF
                     FEHHVAVNLITQYHPHRTDNQGEVLGAYILDCQHNRIHTFLAKCVILATGGAGKTYRY
                     TTNPMVATGDGVAMAYRAGARVGNMEFYQFHPTLLHHHTINNFLISEAVRGEGALLKN
                     PETGERFMKRYAPDQLELATRDVVARAIFSEIEQGQAGYVHLDISHQSKSFLKKRFPQ
                     IYSTLLSIGIDMSQDLIPVVPAAHYQCGGVLTDVDGRTDLARLYAIGEVAFTGLHGAN
                     RLASNSLLEALVMGSNAARCTLKDIATPLKLIDHIPSWSSPGEVNARRASQINAQWRG
                     LRGEMTSYAGIVRTEAGLEDLLQLILKRKKIIEEYYWKHCITRDFIELRNIILNAELI
                     VRAALSRRESRGGHFREDFPNKSFKAQESIAKLGQADSSLG"
     gene            complement(5327..6697)
                     /gene="purB"
                     /locus_tag="I9760_002338"
     CDS             complement(5327..6697)
                     /gene="purB"
                     /locus_tag="I9760_002338"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAT5044097.1"
                     /translation="MTLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWF
                     ESLAANDTIPEVPALDNKARKFLSDLISNFNESEAEKIKEFEKQTNHDVKAVEYYLQD
                     KFQENEQLKSCVAFIHFACTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQ
                     HADVAMLSRTHGQPATPTTMGKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHV
                     AAYPEVDWRKHCANFVTSLGLSFNAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIW
                     SYISLGYFKQKTIAEEVGSSTMPHKVNPIDFENAEGNLGLSNALFIHFANKLTQSRMQ
                     RDLSDSTVLRNLGVAFSYSLIAYHSVAKGNDKLQINKSALQKDLSENWEVLAEAIQTV
                     MRRYNEPNAYEQLKELTRGQMIDAENLKKFIKTLSIPEEAKAELMKLTPETYTGLATQ
                     LVKAFS"
     gene            6850..8553
                     /gene="sulP"
                     /locus_tag="I9760_002339"
     CDS             6850..8553
                     /gene="sulP"
                     /locus_tag="I9760_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HAT5044098.1"
                     /translation="MNTITESYRAGLFQKKYWLQNIISGLIVGVVALPLAMAFAIASG
                     AKPEQGLYTAIVAGLIVSIMGGSRVQIAGPTGAFIVVLSGITAQYGISGLQIATLMAG
                     FILVLFGFARLGSIIKFIPSPVIIGFTSGIAVVIWVGQWQYFFGLPAAGSGHFHEKFW
                     HLLLSFPHMNIATTFLGICALFIVLYSSKLPGLKRIPGPLIALVLVTIIQSVWHFEGV
                     ATIGSLFGGIPQGLPAFTWPEITMGRLIELIGPAFAIAMLGAIESLLSAVVADGMSGT
                     RHNSNRELVGQGIANIVAPLFGGFAATGAIARTATNIRNGGNSPLAGIIHSITLILVL
                     LFLAPLAVDVPLAALAAILFVVSWNMSEARHFIKLIRVAPRADVVILLVTFCLTVFVD
                     LVVAVNVGVIIAVLHFLRRMVNSIQIQRMSEGQLSQELAQEKNYDLPEGVMVYVIEGP
                     FFFGAVETFQSALANTHTDPKVLIIRMRWVPFIDVTGLQAMEEIILNLQKRGVRVLLS
                     EANPLVDTKLRKMGIVQLIGEQNYYKEFSQALDVCRINDSDRKSQPPFEVAKPLASVT
                     Q"
BASE COUNT         2305 a         1678 c         1892 g         2853 t
ORIGIN      
        1 acgcatccct ctcatttaga gagggatgct ctctttgtaa aggctaatga tcttcataaa
       61 aggtgtcagc ctaacaccac tgaggaaatt aaaatatgtc tgttttaaag gctatgactc
      121 atgctgagtg aacaaaggaa tgaacaagcg cctgtttatc gttttcattt taggtttttc
      181 ttccggttta cctatggcat taatcagcag taccttgcaa gcatggtacg ctaataatgg
      241 catgtcagtg cttgcgacgg gagctttaag tttagtcagt ctaccttatg cgtatcgtat
      301 tttctggggt ccaattcttg atcgctactc tttatttcat ttagggaaaa ggcgaagctg
      361 gattctcacc atgcagatac tactgcttct gggttttaat ctgatggcct ggtttattcc
      421 agctcaatat cctaaattga tggcattttt agctttagta ttagcctgtt tttcagccac
      481 acaagatgtg gcaattgatg ctcatcgagc agaatatctt cccccacagg aacatgcatt
      541 gggcgcgtct ctggctgtgt ttgggtatcg attggctctt ttgttatctg gcggtttggc
      601 tttggtaatg gcagaaaaat taggctgggc ttttacttat cgctttatgg gatttgcaat
      661 ggcgataggt atgttggcaa tatttgtcag caaggaaccc tctacagaaa taaaagaaaa
      721 aagtaatttt cttgtttcgt ttattgcacc agtgaaagaa cttttaagcc gcccagggat
      781 tatttatctt ttaatattta tattttgtta taaattaggt gaagcattta ctacgacgac
      841 tagcggtata gtaatgcctt ttttaatcca aggcttgggt ttctctctgg atactattgg
      901 ctatatcaat aaaatgcttg gaatagtctc tatcttgtta ggcggtttaa cggcaggttt
      961 tttattaatg cgatattcct tgtatcgcgc attatttttc tttggcttac tacaagcact
     1021 aactaatgtc ttttttgtca tattagcgat gtcaggaaaa aatattgtgc tgttagcaat
     1081 cgcagtgttt tcggataatt tcgtcgcagg gatgggttct actgcattgg ttgctttatt
     1141 tatgcgtttg gtgaataaac agtttactgg aacacaattt tctatgttag tcgcattatc
     1201 gactttaccc aggatcattt caggtcctgt agctgctacc attcaaatgc atatcggctg
     1261 ggtcggttta tatcagttat ccatcgtttt cgctttgttt tttatacctt ttctagtgtt
     1321 gatcaaggag caaacaaaag agaggttaca aagtgataca gctgctacac caattggtgc
     1381 agcttaattt ctattttaat cagatcaaaa taggttgttt tatacagctc tggactaact
     1441 gaggattaaa taattttagt ttttggtatc tgttgtattg aaattatttg atagtttatt
     1501 gactggtacc gagggtggga ctcgaaccca cacggtgtca ccaccaccgg attttgaatc
     1561 cggcgcgtct accaattccg ccaccccggc acgcgcggta attatagcaa agaaataaat
     1621 tctaacaatg gtttagttca tttattattt gaaaaattac atagccgatt gcattttgat
     1681 tattaatttg acaattgctt gagctttatt aaataatgag atcatatcaa gaataaattg
     1741 gaattatcag gattagaatt gatggagtct ttatgaaaag atattattct acattattag
     1801 ttttggcttt agctcatgcc agtttgagcg cttacgcgca tacatgtcct gatcctcaaa
     1861 ccacgtcatt acaatggggt gtgcctccat caccgtgggt agaaaatcct ttttccccaa
     1921 atagccctca gggagaagaa aatactcgct tcgtgcgagc taatatttta gtcgcaggtt
     1981 atggtcaggg tgtgacttgt acttatagaa actccgtagg tgatttttct attttatggc
     2041 aggtgcggac caaaataccc gctcgtgttg attatagctg gatagaaact atgggagggt
     2101 atgtctgcac acgtggtttg aatgaatgtc agttttatgt ggctgatcct tcggcttaat
     2161 atacccgctt ccttacggtc gcggctcgga tttgataagc actgttgatg caaacccgag
     2221 ccgcgaccat aaggaaacgg acaacacact agctaacacg tattaaaccc tgtcttctgt
     2281 gatattaatc atcatttcca gggctttttt ggcattatta ataacccatt gctgatcgtt
     2341 gactggcagt ctgtttctgg ttcctgtgaa ttcgttgacc acatcacctg cttttacttc
     2401 attgtaaggc atagatttgc cctggcgaag taaatcgagg agagcgacca aatgtggtgg
     2461 atcattgcga gacatggtag cacaaccaca tccaactcct ttttcagtgt ctttttgagg
     2521 ggcttcagca agatttacaa cacgaattcc aaggttttta caatattgct ttaagttttc
     2581 taccatgtgg ttttcagttc cgatggcata actttttgta cctgctttgt catggactac
     2641 cagatcccat atgaatgcag tagaacctga gtaatcagcc actttgatga catcattacg
     2701 gcactctgga tgtacaatgg tagtgtatcc ctggcttttc caatagtcac acatttcagg
     2761 ttgataatgg gtatgaactg cgcattgact tgcgaataaa atcaactctg ctttatcaaa
     2821 ttgcttgagt gtttttttgt cttgcgctgg tagtgaataa ttagcgccgg cagttcctcc
     2881 aggccaataa gcgagatttt taattcctaa ccagtaagcg acattttctc ccatgtgttg
     2941 atcaggaata aacaggattt tcttgttttg tttttgagcc cattgaaaaa tttttttgac
     3001 attggaactt gtacaaaccg cacctccctg ggcaccggtc atggctttca cacgaccaga
     3061 ggtattcatg tagcacacag gtaaaatatt ttcagcacca taacgctcat ttaaatcaag
     3121 gaaagcaggc tcgaccataa agtctttggc aagcatttcc atggtgcagc ctgacttggg
     3181 attggtgatg tagacgtgtt gattttcatt agccaaaatg cttatggatt ccgccataaa
     3241 atgaactgct gattccacta tgactgattt ttcagggtta ttcgcagcca tcaaagccaa
     3301 ttggtaggag tcaccgattt tgcccccgaa ttgctctatt aatcgaacaa tttctccacc
     3361 catataataa tgggcaagaa gtaacagttt gttaccgaaa tgattttggg ctttagcaag
     3421 ataaggtttc atccaattta aaacagtcgt aagttttcta tcgggtaaag cctgatattc
     3481 ctgggcataa ggaataaaat cttcttgata ccaatctaat ggataatcgc tttgacatat
     3541 agccatatca ttacgaatag ccatgagggt cgcttcgggt tggtaagtag ttgaattttt
     3601 aaacattaaa tctacctcta gtcattaggg atttgtggga aggattcttt cattattgtt
     3661 aatgggatag ctttgccagg ctatcctaat gaactgtctg cctgtcccag ttttgcaata
     3721 ctttcctggg ctttgaaact tttattgggg aaatcttcac gaaaatgtcc accacgtgat
     3781 tcccttctgg aaagggctgc tctaacaatc aattctgcat taaggataat gttccttaac
     3841 tcaataaaat ctctggtaat gcagtgtttc cagtaatact cttcaataat tttcttacgc
     3901 ttcaatatta attgtagtag atcttcaagg cctgcttcag tgcgcacaat gcctgcatag
     3961 gaagtcattt cccctcttaa gcctctccat tgagcgttaa tttggctggc tcttcttgca
     4021 ttgacttcac ctggagagct ccagctgggg atatgatcga taagtttcag cggagtagca
     4081 atgtctttga gtgtacagcg tgccgcattg gaacccatga ccaaggcttc aagtagggaa
     4141 ttgcttgcca gcctgttcgc accatgcagc ccagtaaaag ccacttcacc gatagcgtat
     4201 aatcgtgcca gatcggtgcg gccgtccaca tcagtaagaa ccccaccgca ttggtaatga
     4261 gcggccggaa cgacaggaat caaatcctgg ctcatatcaa ttccaataga taacaatgtg
     4321 gaataaattt gtgggaaacg ttttttcagg aaagatttgc tttgatgaga aatatccaga
     4381 tgcacatatc cagcctggcc ttgctcaatt tcactaaaaa tagctcttgc cacgacgtct
     4441 ctggttgcta actcaagttg gtcaggtgcg tatcgtttca taaaacgctc gcctgtttca
     4501 gggtttttaa gcaaagcgcc ttcgcctcga actgcttcgg aaattaaaaa attgttaatg
     4561 gtgtgatggt gcaagagggt agggtgaaat tgataaaatt ccatgttgcc cacacgagct
     4621 ccagccctgt atgccatcgc gacaccatca cctgttgcaa ccatagggtt agtcgtatac
     4681 ctgtaggttt tgccggcgcc accggtcgct aaaatgacgc atttcgcaag aaaggtgtga
     4741 atgcgattat gttggcagtc gagtatatag gctcctaata cctcaccctg gttatctgtc
     4801 cggtgaggat gatattgagt aatcaggtta accgccacgt gatgttcaaa aaactggatc
     4861 tgttgatgtt gccttgccaa atgattgaga gtttgtgtta gcgttaatcc cgtttgatcg
     4921 ccacaattaa aaatgcgccg gtgtgaatgg ccaccttctt tggcaagatg aaagcgacca
     4981 tcattttctc ttttaaattg aactttatac tgatcaagtt gttttataat atctggccct
     5041 tggcggataa taaattccac cgcaggtaag tagcatagcc catctccagc caatacagta
     5101 tcagatatat gagactccag tgaatcttct ggcgaaatgg cggcagcaat ccccccttga
     5161 gcatagcggc tgttacactc aatggactct gctttgctga ttaatgcaat gtttagcttg
     5221 ggttggatag ctaaaagctg caaacaataa tgtaacccgg ctaatcctgt tccaatgacg
     5281 agaacatcga attcgaacga ttggccttgt tgtgatagct gatcactcat gaaaaagcct
     5341 tgaccaattg ggttgcaaga cctgtgtaag tctctggagt taatttcatt aactctgctt
     5401 ttgcttcttc gggaatagac agggttttaa taaatttttt aaggttttca gcgtctatca
     5461 tttgtcctcg tgtaagctct tttaattgtt cataggcatt gggttcattg tagcggcgca
     5521 tgacagtttg tatggcctct gctaaaactt cccagttttc ggataaatct ttttgtagag
     5581 ctgatttatt gatttgtaat ttatcattac ctttggctac tgagtgatag gcaatcaaac
     5641 tgtaagagaa ggctacgccc aagttacgta agacagtcga atcagacagg tctctttgca
     5701 tgcgtgattg agttaatttg ttggcaaaat gaataaataa agcattagac agacctaaat
     5761 tgccttcggc attttcaaag tctataggat ttaccttgtg cggcatggtc gatgagccta
     5821 cttcttcggc aattgttttt tgcttaaagt agccaagaga aatatagctc catatgtctt
     5881 gggtataatc cagaagaatg ttattaattc tcaccataat ctgggagact tccgctatgc
     5941 catcatgtgg ttcaatttga gtggtatagg cgttaaagga taaacctagg gatgtaacaa
     6001 aattagcgca atgcttacgc cagtccactt cgggataggc tgctacgtga gcattgtaat
     6061 tgccaacagc tccattaaat ttggcaggga ttaacacttc tgccaattgt tgctggggtc
     6121 gtttaaggcg agctacaaaa ttgaccaatt ctttacccat ggtggttggt gttgctggtt
     6181 gaccatgcgt tcttgagagc atcgcaacgt cagcatgttg ttttccaaga agcgtaatgc
     6241 ttcccatgat ctcagctata gtaggttgta taacttgggc tatcgcttgt ttgatcatta
     6301 aagcataagc caggttattg atgtcctctg aagtgcaggc aaagtgtatg aaagcaacgc
     6361 aagatttgag ttgttcattt tcctgaaatt tatcctgaag ataatactct acagctttaa
     6421 cgtcatgatt ggtttgtttt tcaaattctt ttattttttc agcttctgat tcattaaaat
     6481 tggaaataag atcagataaa aatttacgag ccttgttatc caaagcaggt acttctggaa
     6541 tagtgtcgtt agcagctaat gattcaaacc acttgatttc aaccattaaa cgataatagg
     6601 ttaatgcaaa ctcactaaaa tagggactta atgctctggt tttattgaca taacgaccat
     6661 ctatgggtga aatcgcgttt aatgcagtaa gtgtcatgat aggtagctta ttccaatatt
     6721 aaagcgtata taatattaga tgcaggacaa gatgtgaatt aaagcgataa atattctaga
     6781 aaattagcgt aaatttgttc aaagtgcgta taataatgac catgttttta atgggacagg
     6841 ttattattta tgaacacgat cacagaatcg tatcgagccg gtttatttca aaaaaaatat
     6901 tggttacaaa atattatttc tggcttaatt gttggtgttg ttgctttacc tttggcaatg
     6961 gcttttgcaa tagcttccgg ggctaaaccg gaacaaggat tatataccgc tatcgttgcc
     7021 ggattaatag tctcaattat gggtggcagc agagttcaaa tcgccggtcc tacaggtgct
     7081 ttcattgtcg ttctttccgg gataacggcc caatatggta tttcagggtt acaaatagct
     7141 actttaatgg cgggctttat cttggttctg tttggctttg cccgtctggg atccattatt
     7201 aaatttattc ccagtccagt aatcatcgga tttacttcag ggattgctgt cgttatttgg
     7261 gtagggcaat ggcaatattt ttttggttta cccgcagctg gaagcggaca ttttcatgaa
     7321 aaattttggc atttacttct gtcttttcct catatgaata tcgcgactac ttttttaggt
     7381 atttgtgctt tatttattgt actgtattcc agtaaattac caggactaaa gcgtattcct
     7441 ggccctttga ttgctttggt actggttaca atcatacagt cagtttggca ctttgaggga
     7501 gtggccacta taggcagcct ctttggagga ataccacaag gcttgccggc tttcacctgg
     7561 cctgaaatca caatgggacg actgattgag ttgattgggc cagcctttgc tattgcgatg
     7621 ctgggagcaa ttgagtcctt attgtctgct gtggttgcgg atgggatgag tggaaccaga
     7681 cataattcga atagagaatt agttggtcaa gggatagcta atattgtggc gcctttgttt
     7741 ggtggttttg ctgccaccgg agcgattgcc cgcacggcca caaacatacg caatgggggc
     7801 aatagtcctc tggcaggaat tattcacagc attactttga ttttagtcct gttgttcctg
     7861 gctcctctgg cagtggatgt acctctggcg gcattggctg ctattttatt tgtggtttcg
     7921 tggaatatga gtgaagcccg ccactttatt aaattaattc gagtagctcc tcgtgctgat
     7981 gtagtcatct tattggttac tttttgtctg acggtgtttg ttgatttggt tgttgctgtc
     8041 aatgtagggg tcatcattgc cgtcttgcat ttcctgcgcc gaatggttaa cagcattcaa
     8101 attcagcgta tgtcagaagg acagttatct caagagttgg cacaagaaaa gaattatgat
     8161 ttgcccgaag gggtcatggt gtatgttatc gagggcccgt ttttctttgg tgctgtcgaa
     8221 acatttcaga gtgcgttggc taatacccat actgatccca aagttttgat catcagaatg
     8281 cgttgggttc cgtttataga tgtcactggt ttgcaggcta tggaagaaat tattttgaat
     8341 ttgcagaagc gtggagtcag agttttgctc agtgaggcta acccgctcgt tgatacgaaa
     8401 ctccgtaaaa tgggaattgt tcaattgatt ggagagcaaa attattataa ggaattttct
     8461 caggctcttg atgtttgccg aattaatgat tctgacagaa agagtcagcc tccctttgaa
     8521 gttgcaaagc cgctggcttc agttacccag tgagctaagc tggttatctg ttcaagttga
     8581 ggaagcgctt cctcaactcg aatggataga attaacgtga taatcgaaaa acttcaaaga
     8641 ttgccatgcc tgtttttata tgtaattcaa tttgctaaac ttaaaatcta tttttttggt
     8701 ttgacactac gaataatttt tcccggcc
//
LOCUS       DACTJE010000139         8719 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.139, whole genome shotgun sequence.
ACCESSION   DACTJE010000139 DACTJE010000000
VERSION     DACTJE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.139"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            308..2407
                     /gene="pieA"
                     /locus_tag="I9760_002340"
     CDS             308..2407
                     /gene="pieA"
                     /locus_tag="I9760_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PieA/LirC"
                     /protein_id="HAT5044099.1"
                     /translation="MQEKIINLGKGLKKVNEGIKKHISRAEEFLYLANQWEKENIKIN
                     DLLNGVQFTVNDKKMSGQEIKEELSNLIERYNNFLKKNLKGERSETLELKEIQQQLTN
                     LYNAVNDIYTYLSINKTEFFLEAIKSCYKKDQRMGSTIQFVLKIADHLSKERLQFNLN
                     LNSQFAEASESFQEVTTKFYREVATPRIESAQKLLELMNNFVDQYISQGASPEDAIKK
                     LKPFEKYFTIEISHYEEFGHLVYKTNFNCNPALFDVNPIEFLNELFVQFYKDTTLSSR
                     SKTALKQVLDKFEITYEVPKTWYGYIGWDIPIVTDKNSVLVTYDKQAYYRYHQESGQR
                     EMEFFETIERCLDKSKDIIIATKKENLKDQKEFSKLIDKAIKELEETKKKRIFQKEPH
                     ELVTSIINKSIKLLQDLKNNMPLRREDLVMVILSTENALNAAKSKLKPQEINLSEPEK
                     CIKNFKKILAIKQKEELCFYKSDEEQSIFSKHINSELRELVESYQVQKRSEKELVIEP
                     EKMNSPQRIEPKEEDKKIDLNKKMNLYRVQKIMKNFVFEYCGYKGGKKVKEGGATIEE
                     LKNKLKDFRDYFQIEIVMNDKSGSEELNIIFNYQNIKNKNPHDLFNELFKKFTEDIGY
                     SGMGSRSIEALKRALAKNNITYTNGYYGKTATLTSLLWSKEPPVITDEKNIFKLSDEK
                     HLSENKEDIRGKGITNI"
     gene            complement(2473..3039)
                     /locus_tag="I9760_002341"
     CDS             complement(2473..3039)
                     /locus_tag="I9760_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAT5044100.1"
                     /translation="MKKIICLLSNLVLTVGIMSAHAETAIIKTSEGNITCELFTKEAP
                     NTVANFVGLATGTKEFKDIKTGKMVKRPFYNGLTFHRVIAGFMIQGGDPLGNGTGGPG
                     YTFDNENTNASFNKPGVLAMANAGPNTNGSQFFITVAPTPELQGGYNVFGQVISGQEV
                     VDKISKMPTDPQDKPITPVVIENITIQK"
     gene            3185..4699
                     /locus_tag="I9760_002342"
     CDS             3185..4699
                     /locus_tag="I9760_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAT5044101.1"
                     /translation="MSDSEYESDSESDSDSKSEYELRTLPSKIHFVWVGGPIRAQYLR
                     TIMDVAAVALKSGFEVNLWVDNEMNYIKTSTQEGIDIPNLRIRNVHELEEGMRNAPFY
                     EEGDRYKKFWEYVNRERVGFKNLAAAADFFRFEILRQEGGYYFDTDIVFYLDENSQFV
                     ADEVAFGIKAHVDLHTGSFTDPRGGSTTHYVNTLGDVNNDIIAAIPDHEVMEDAILNA
                     MQRHEEYDSSPLPLESLKKKFGPYGNLMDAKRTPYQLKEPMDFRKYQTIAAGPGALQE
                     ALRDYMEGVEVEDSSLLVTLKVGNQYSNVANDKDKKPIMGIEVVSKCDKTWLTKKRKA
                     AQKAFDTDSIVIEPSLSKESGHPVSNREAYSDDDEEYSDELTEETGFHGNLRKEQFIN
                     ELKDYIIIKKEEARQALEVKENEEGHDSEEKILSKHFKTRHIEITKGFSVEQKIAAVE
                     KLISLLQEKDGAELLNAIDYAALTSSRLGKLVERYDDLILKEYVVPDEYGEKPY"
     gene            complement(4736..5509)
                     /gene="lirA"
                     /locus_tag="I9760_002343"
     CDS             complement(4736..5509)
                     /gene="lirA"
                     /locus_tag="I9760_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LirA"
                     /protein_id="HAT5044102.1"
                     /translation="MFFSKDEKNPIKRALQGELLQNEPFIQLCTKIENYLMDTEAVNE
                     QLIELNEQLTMRLKEKGLKPGEKGATKQLRTLIQEILTEAGFREGMLQTIGNKPLKKE
                     DFMFLVSSGFMLKDSSLRASSHGELTHAIQWCLIILKQKKDSSFLENIPTSEICDRIY
                     KKLGHQDSSNPNYPFTCWDVLIDKLGEIDSRSPEWLSDHIQNDEDQIFPVLREVIKNR
                     TEKGKTEENKGKLQKKLENPPEHYEKHEEIENILMPKPK"
     gene            complement(5694..7688)
                     /locus_tag="I9760_002344"
     CDS             complement(5694..7688)
                     /locus_tag="I9760_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1959 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044103.1"
                     /translation="MLVSNTILKAALSHFEDRSDLIKQLDLSSDNDKNSFYKWVLAFW
                     LGSNITREKIIKHPLYIKCEVFIDLGYSCILVLDKQMSLLKQNSLAYKILQKNLFEII
                     QYYNVNYPLLTQNPQTLFSFFANILAKIDSKVCEDISHRKILELTQNTCLAELSRTQN
                     GPPDGLAATQVSNDVTSLMKVNPFNLGVAINKLITENFSKELFFPHYIKPTTDKHITH
                     KLLIPAWDGIVLEGLSVKERKTTNNTVVLALIGHFQTEHHYLNTSFHEFQELFGTELV
                     LINHRNYSNRSNKFANSAEEIARDVLAFAKHFRQKNKKIVLYGMCGGAAHMILAAHML
                     SHQKIPFKLIVDRFSQKYINFVDFKTLSRARDFSHSNGQDCSRLLPGYKYYPGLMPYL
                     LILLFLLLFILVQLGLFLTKTNIDFAKLVRRIPEEDLLILQAKGEKIAALKKPFFTDI
                     IVHPENDMRAAVKDKRKQRKTILKNLCEHCLHAAGQAVFSAEMQKIFLQLFNCFDQCL
                     QLINNEKLMENTITNRPVDLHSKKLYTLTTRNKLPISQFIRGFFKQSPKMHAHLLDSI
                     KPYSSHLIVDALKQIYGNHPSMHSNLLQFSNHLALLLNDMKTNQFFISYMADRLSATQ
                     LADLNEPINALLRSELLQLIFKSSSEQNNQNIINNHRVSI"
     gene            complement(7941..>8719)
                     /gene="legL5"
                     /locus_tag="I9760_002345"
     CDS             complement(7941..>8719)
                     /gene="legL5"
                     /locus_tag="I9760_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL5"
                     /protein_id="HAT5044104.1"
                     /translation="ELAMAFAHIPAFVNTLRLGDNNLGNKSGEELAEIFAKIPLSVTS
                     LDLRYNELGKRNGKELAIAFAKIPSSVTNLNLKFNHLGKITGEELAMAFANIPTNVDS
                     LVLANNELGAKSGEELAKIFSKIPASITSLNMGNDWFAVIEKLSQQKMVLSTITHVVI
                     DYSSAQEHSAKLKLLATVFPNLNKVTFMDIHGNNALAGQNPIEEAKWLKLCGIKNSVP
                     SLLSQALFFVAKNKEHRSIQDYPSLLPKELTDSISSLLKC"
BASE COUNT         2932 a         1332 c         1637 g         2818 t
ORIGIN      
        1 gaattttaat aaattatgag ttagcagagt accataagta agctattatc taaaatattg
       61 attttaaatg gttaattacg atttttgttg agttttttac ctctagctat ctttaattac
      121 caaaataact tatcaaatta aacatatcaa taaaatcttc tattaatccc gaacaatatc
      181 tttcccaagc aatgattttt ataaatccat tgcgtttatg aaaaataata tgattaaaaa
      241 ttaatttaat tgtttattaa attaactata attaaaaaat aagtcacatt gaacctggaa
      301 ttatgtaatg caagaaaaaa ttatcaactt agggaaaggg cttaaaaagg ttaatgaagg
      361 cattaaaaaa catatttccc gagcagaaga gttcctctac ttggcgaacc aatgggaaaa
      421 agagaatatt aagattaatg atttattgaa tggagtgcag tttacagtta atgataaaaa
      481 aatgtcgggt caagaaatta aagaagaact atctaatcta atagaaagat ataataattt
      541 tctaaaaaaa aacttaaaag gggaaagaag cgaaacatta gaactaaagg agattcagca
      601 acaacttact aatttataca atgctgttaa tgatatttat acttatttat ccataaataa
      661 aacagagttt tttctcgaag caatcaagtc ttgctataaa aaagatcaga ggatgggtag
      721 tacaatccag tttgtgctga agatcgcgga tcatctttct aaggagagac tgcaatttaa
      781 tttgaattta aattcgcaat ttgcagaagc ctccgagagt tttcaggagg taactaccaa
      841 attttacaga gaggttgcta ctcctagaat agaatctgca caaaaattgt tggaattgat
      901 gaataatttc gtagaccaat atatatcaca aggagcaagt ccagaagatg caataaaaaa
      961 acttaagcca tttgagaaat attttactat tgaaatctcg cattatgaag agtttgggca
     1021 tttagtatat aaaacaaact ttaattgtaa tccagcgcta tttgatgtta accccattga
     1081 atttttaaat gaattatttg tgcaatttta taaagacacc actttatcat ctcgctctaa
     1141 aacagcttta aaacaagtgc ttgataaatt tgagattact tatgaggtgc caaagacatg
     1201 gtatggttat attggctggg atatccctat agtaactgat aaaaattcag tattagttac
     1261 ttatgacaaa caagcttatt acagatatca tcaagaaagc ggccagagag aaatggagtt
     1321 ttttgagaca attgagcgct gtttggataa gtcgaaagac attattattg cgactaaaaa
     1381 ggaaaattta aaagaccaaa aagagttttc taaattgata gataaggcta ttaaggagtt
     1441 agaagaaaca aaaaagaaaa gaatttttca aaaggagccg catgaattgg taacaagtat
     1501 tatcaataaa tcaataaagt tgctgcaaga cttgaaaaat aatatgccat taagacgaga
     1561 agatttggtc atggtaatct taagtactga aaatgcttta aatgcagcaa aatcaaaatt
     1621 aaagccacaa gaaatcaatt tatccgaacc ggaaaaatgc attaaaaatt ttaaaaagat
     1681 attagcaata aaacagaaag aagagctttg tttttataaa tcagatgaag aacaatctat
     1741 tttctccaaa cacataaatt ctgagcttag agaacttgtc gaatcttacc aggttcaaaa
     1801 gagaagtgaa aaagagttgg ttattgagcc agaaaaaatg aattcaccac agagaataga
     1861 accaaaagaa gaagataaaa agattgattt aaataagaaa atgaatttat accgtgttca
     1921 aaaaattatg aaaaattttg tatttgagta ttgcgggtat aagggaggga aaaaagtaaa
     1981 agagggagga gcaacaatag aagaacttaa aaataaactc aaagatttta gagattattt
     2041 tcaaatagag atagtgatga atgataaaag tggttcagaa gaactcaata tcatattcaa
     2101 ctatcaaaac atcaagaata aaaatccaca tgatttattt aatgagctct ttaaaaaatt
     2161 taccgaagac ataggatatt ctggtatggg ttcacgatca atcgaggctc ttaaaagagc
     2221 attagctaaa aataatatca cttataccaa tggatattat ggaaaaacag cgactttaac
     2281 aagcctatta tggagtaaag agccacctgt tattactgat gaaaaaaata tatttaaatt
     2341 atccgatgaa aagcatttaa gtgagaataa ggaagatatt cgcggaaaag gaattacgaa
     2401 tatctagtcc ttacataccc atatttcgaa ttttaattgg cttacacaat atcacaagag
     2461 ttggtatcac atttactttt gaatggtaat attttcaatg actacaggtg taatgggttt
     2521 atcttgtgga tcagtgggca tcttgctaat cttatcaaca acttcttgtc cagaaatgac
     2581 ttgaccaaaa acattgtagc caccttgtaa ttcaggtgtt ggtgcaacgg taataaaaaa
     2641 ttgactgcca ttcgtgtttg gtccagcatt ggccatggca agtacgccag gtttgttaaa
     2701 actggcatta gtattttcat tatcaaatgt gtaacctggg ccaccggtcc catttccgag
     2761 ggggtcgccg ccctgaatca taaagccggc aatcacacga tgaaaggtta agccattgta
     2821 aaaagggcgt ttaaccattt tgccagtttt tatgtcttta aattcttttg ttccagttgc
     2881 cagccctaca aaatttgcaa cggtattggg agcttctttt gtaaataatt cacacgttat
     2941 gttgccctct gaagttttaa taattgcagt ttctgcatgt gctgacatga tacctactgt
     3001 taacactaag ttactgagta aacaaataat ttttttcata tgaaactctc aatagaatgg
     3061 ttaagaccag tataacagta ttgtatcgca ttaaaatggt ttttaaatga aactcagagt
     3121 ttcggctgaa ttagatgggg gtgtactata attaaagaac attgaattgt tatgcgagag
     3181 tttcatgagc gattctgaat atgaaagtga ttctgaatct gatagcgatt ctaaatctga
     3241 atatgagctc cgtacgctac catccaaaat acattttgtt tgggttgggg gacctattcg
     3301 agctcaatat ttacgcacta ttatggatgt tgctgcagta gcactaaaaa gtggttttga
     3361 ggttaattta tgggttgata atgaaatgaa ttacattaaa acatcaactc aagaaggaat
     3421 agacatacct aacttaagaa taaggaatgt tcatgaattg gaagaaggga tgcgaaatgc
     3481 ccccttttac gaggagggag acaggtacaa aaaattttgg gaatatgtca atagagaacg
     3541 agtcggtttt aaaaatcttg cggcagccgc agattttttt cgcttcgaaa ttttaaggca
     3601 agagggcggt tattacttcg ataccgatat cgttttttat ttggatgaaa actcacaatt
     3661 tgtagcagat gaggttgctt ttggcataaa agcgcatgtg gatttgcata caggatcatt
     3721 cacggatcca cgtggaggtt ctactaccca ttatgtcaat actttaggag atgttaataa
     3781 cgatattatt gctgctattc ctgatcatga ggttatggaa gatgcaattc taaatgctat
     3841 gcaacgtcat gaagagtatg attcatcccc attgcccctg gaatcgctta aaaaaaaatt
     3901 cggtccatat ggcaatctga tggatgctaa acgcactcct tatcaattga aggaacctat
     3961 ggattttaga aaatatcaaa ctattgcagc tggtcctggt gcattacagg aggcattgcg
     4021 tgattacatg gaaggtgttg aagtagaaga ttccagcttg ctagttacct taaaagttgg
     4081 taatcaatac tcgaatgtag caaatgataa ggataaaaaa ccgattatgg gaatcgaagt
     4141 ggtcagtaag tgtgataaaa cttggctgac taaaaaaaga aaagctgcgc aaaaagcttt
     4201 tgatactgat tcgattgtta tagagccctc actttcaaag gaatctgggc acccagtaag
     4261 taatagagag gcatactctg atgatgacga agagtatagt gatgaattaa cagaagaaac
     4321 cggattccat ggcaatttgc gcaaggaaca atttatcaat gaattgaaag attatattat
     4381 cataaaaaaa gaagaagcaa ggcaggcttt ggaagtgaaa gagaatgaag aaggccatga
     4441 ttctgaggaa aaaattctaa gtaagcattt taaaacacgt cacatagaga taacaaaagg
     4501 gttctctgtt gaacagaaaa ttgctgccgt agagaagctt attagtttac tgcaggaaaa
     4561 ggatggggcg gaactgctta atgcgattga ttatgcagcg cttaccagtt ctcgcctggg
     4621 taagttagtc gaaagatacg atgatttgat acttaaagaa tatgttgtgc ctgatgagta
     4681 tggggaaaaa ccatactgaa ttcaaatgga ttagttggtc tgtagtagtt caaatttatt
     4741 tgggttttgg catgagaata ttttcaattt cctcgtgttt ttcataatgt tctggcggat
     4801 tttccagttt tttttgtaat tttcccttgt tttcttctgt tttgcctttt tcagttctgt
     4861 tttttattac ctctcttaac acaggaaaaa tttgatcctc gtcgttttga atatgatcgg
     4921 aaagccattc cggggatctg gagtctattt ctcctaattt atcgataagt acatcccaac
     4981 aagtaaaagg ataattagga ttcgaagaat cttggtgccc tagtttttta taaattcggt
     5041 cacaaatctc actagtaggt atgttttcaa gaaaactgct gtctttcttt tgtttcaata
     5101 tgattaaaca ccattgaatt gcatgggtca gctcaccatg ggatgatgct cttagactgc
     5161 tgtcttttaa cataaatcct gatgatacta aaaacatgaa gtcttctttt tttaatggct
     5221 tattccctat tgtttgtaac attccctctc taaagccagc ctcggttagt atttcctgta
     5281 tgagtgttct taactgcttt gttgctcctt tttctccggg tttgagtcct ttttccttta
     5341 acctcattgt cagttgttcg tttaattcaa taagttgctc gtttacagct tcggtatcca
     5401 tcaaatagtt ttctattttt gtacatagtt gtataaaggg ctcattttgt aataattcac
     5461 cttgaagagc tctttttatt ggattttttt catctttaga gaaaaacata tgacctccaa
     5521 cgcgctaatt tttttaagta tagattattt ttaataaata ttgtttataa atattcgtta
     5581 ttgcttactt attggttgac ttgagtgttg atgagcacat ggtttaagcg catttacatt
     5641 aatcttgcca tttagtatca acgataaccc aattagtggg gtcaatgaat gcattaaatg
     5701 gataccctat gattatttat gatattttga ttattttgtt cagacgaact cttgaaaatt
     5761 agttgaagta gttcgcttct taacagcgca ttgataggtt catttaaatc agctaattga
     5821 gtagcagata aacgatcggc catataagaa ataaaaaact gattggtttt catatcattt
     5881 aaaagcaaag ccaaatgatt agaaaattgc aataaattag aatgcattga tgggtggttt
     5941 ccataaattt gttttaaagc atcaactata aggtgactag agtatggttt gattgaatca
     6001 agcaaatggg catgcatttt tggtgattgt ttaaaaaaac ctctaatgaa ctgactgata
     6061 ggtaacttat ttctggttgt aagcgtgtaa agttttttac tgtgtaaatc tacagggcga
     6121 ttagtgatgg tattttccat taatttctca ttgtttatta gctggaggca ttgatcaaag
     6181 cagttaaata attgaagaaa aatcttttgc atctcggctg aaaaaacagc ttgaccagca
     6241 gcatgcaaac aatgctcaca taaatttttc aagatggttt ttctctgttt tcttttatct
     6301 tttacggctg cccgcatgtc attttcgggg tgtacaatga tgtctgtaaa gaatggtttt
     6361 tttaaggctg ctattttttc tcccttggct tgtaaaatca ggaggtcttc ttcaggaatc
     6421 ctccgcacaa gttttgcgaa atcaatattt gtttttgtta aaaatagacc taattgaact
     6481 aaaataaaca acaataaaaa aaggagaatg agcaagtatg gcattaaacc aggataatat
     6541 ttgtaacctg gaagcaatct cgagcaatct tgtccgtttg agtgtgaaaa atctctggct
     6601 ctcgagagtg ttttaaagtc tacgaaattg atgtattttt gagagaacct gtcaacgatt
     6661 aacttaaatg ggattttctg atgggatagc atatgggccg ctaaaatcat atgcgcagcc
     6721 ccgccacaca ttccatacag tacaattttt ttgttttttt gtctgaaatg ttttgcgaaa
     6781 gcgagcacat ccctggctat ctcttcagca ctgttggcaa atttattgga ccgattagaa
     6841 taatttctat gatttataag cactaattct gtgccaaaaa gttcctggaa ctcatgaaag
     6901 ctggtattaa gataatgatg ttctgtttgg aaatgtccaa ttaacgctaa aacaacggta
     6961 ttgtttgttg tttttctttc cttaacagaa agcccctcaa gtacaatacc atcccaggct
     7021 ggtattaata atttatgagt aatatgcttg tcggttgttg gctttatgta gtgtgggaaa
     7081 aaaagttcct tgctaaaatt ttccgtaatc agcttattga tagcgacacc taaattgaac
     7141 ggattaactt tcattaaact ggtaacatca ttagacactt gtgttgctgc caatccatct
     7201 ggcgggccat tttgagtacg tgataattca gctaggcacg tattctgagt cagttcaagg
     7261 atttttcggt gagaaatatc ttcacatacc ttgctgtcaa tttttgcaag aatattggca
     7321 aagaaagaaa atagcgtttg gggattctgg gttaataacg gatagttgac attataatat
     7381 tgaattattt caaataagtt tttttgcagg atcttataag caagtgaatt ttgctttaac
     7441 aagctcattt gtttatccaa aaccaaaatg cacgagtaac ccaaatcaat aaaaacttca
     7501 cattttatgt acaaagggtg tttgataatt ttttcgcgag tgatgtttga acccaaccaa
     7561 aacgccaaaa cccatttgta aaacgagttt ttatcgttat cagaagacaa atccagttgt
     7621 ttgattaaat cacttctgtc ttcgaaatgg cttaatgccg ccttgagaat tgtattggaa
     7681 actaacatga gttcaaaaaa tagtaaaaaa caccgtatta tagcaatttt atattcagaa
     7741 tttgcaaaaa tatttcaaat gtgggtaagt ttttctgtgg aaagtaattc aggtaacctg
     7801 atattttctg caataccact acctatacaa tcggtggatg aagttagatt tacatcataa
     7861 agatggtacc atcattaaaa tggcaccacg tgaaatggat aattattgag ctgatccgtg
     7921 taagaaatac aaatcttgtt ttagcatttc aatagcgatg aaattgagtc agtaagctct
     7981 ttaggaagca ggcttgggta atcttgaatg gacctatgtt ctttattttt tgcaacaaaa
     8041 aacaaggctt ggctgagtag ggagggcacg gaatttttaa tgccacataa tttcagccat
     8101 ttagcttcct ctataggatt ttgaccagct agggcgttat ttccatgaat atccataaaa
     8161 gtgactttat ttagattagg aaaaacagtt gcaagcaatt tgagttttgc tgagtgctct
     8221 tgagctgagc tgtaatctat aaccacatgg gttattgtag atagaaccat tttttgctga
     8281 cttaattttt ctatgacagc aaaccaatcg ttgcccatgt tcaagctggt tatagaagct
     8341 ggaattttac taaaaatctt ggctaattcc tctccacttt tagccccaag ctcgttattc
     8401 gctaaaacga gtgaatcgac attggttggg atgttggcaa aggccatagc cagctcttct
     8461 ccagttattt taccaaggtg attaaatttt aaattaagat tagtcactga gctgggaatc
     8521 ttggcgaaag caattgctaa ctccttacca tttctttttc caagttcatt gtagcgtaaa
     8581 tcaagtgaag tcaccgatag tgggattttg gcaaagatct ctgctaattc ttctccactt
     8641 ttattgccga ggttattatc tcctaatcga agagtattca cgaaagctgg aatgtgtgca
     8701 aaagccattg ccaattcct
//
LOCUS       DACTJE010000140         8634 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.140, whole genome shotgun sequence.
ACCESSION   DACTJE010000140 DACTJE010000000
VERSION     DACTJE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.140"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            5..298
                     /gene="flgM"
                     /locus_tag="I9760_002346"
     CDS             5..298
                     /gene="flgM"
                     /locus_tag="I9760_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HAT5044105.1"
                     /translation="MVNQINDSANLRHIDMDNRINANHKEVKSPVLENNSTDSVNLSS
                     TSKQLEALKASLKDLPEINEARVLYFKAEIQSGQYEIDSSKIAHGMLNSVEMV"
     gene            315..812
                     /locus_tag="I9760_002347"
     CDS             315..812
                     /locus_tag="I9760_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HAT5044106.1"
                     /translation="MNNNNKAKTLITQLEQEISWVEELNTLLSEEKEILSTSQFNQLE
                     TLAEKKQELSTKLEASAKQRVDLIKESNPGDKPHGLLLSDFLKNCATEDGIQINHLNN
                     TLAEKLNLCRELNTINGQVIANNMYTRQQIVNALSGNKSNAVSVYTSNGNLETPSESS
                     HHEEA"
     gene            complement(875..1618)
                     /locus_tag="I9760_002348"
     CDS             complement(875..1618)
                     /locus_tag="I9760_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAT5044107.1"
                     /translation="MNFDNQIALVTGGVSGMGKACVQYLQQHGMKVVVWDKQEGTSNE
                     AELYVACDVTSDESVEKAMRQTISQVGVPRVCINCAGIAPAKRMVGKEGPMPLESFRQ
                     VIDVNLIGTFNVMRIAAHAMSGLELDNKSQERGVIINTASIAAFEGQIGQSAYSASKG
                     GIVSMTLPAARELAQFAIRVNTIAPGLIATPLLLNMPQEVQDSLVATVTFPKRLGRPE
                     EFASLVGHIIENQMINGEVIRLDAALRMR"
     gene            complement(1827..2372)
                     /locus_tag="I9760_002349"
     CDS             complement(1827..2372)
                     /locus_tag="I9760_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT5044108.1"
                     /translation="MLLNRADSLLVLIDVQEKLTPAILNLDLFLARCEWLLKLARKLD
                     VPVLASEQYPKGLGRTVSPLSSYINQEECIEKIHFSCMQEPQYINLLKGYNKNQLILI
                     GIEAHVCVLQTAIEMKGYGFEVFVVVDGVSSRSEFDLKYGLKRMKQNGIHLITAEMVL
                     FEWLKQAGTPEFKAISKEFLQ"
     gene            complement(2492..3583)
                     /locus_tag="I9760_002350"
     CDS             complement(2492..3583)
                     /locus_tag="I9760_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HAT5044109.1"
                     /translation="MEMMRQQRNLLLIPFILCFILARPVFSLNLDALLPDERNTVEVF
                     QKASSKVVYVHRLANATVQRRYSLQKTHIPDGAGSGIIWDNKGHVVTNFHVINGADDI
                     AITLGNMTVPAKVIGSEPRKDIAVLEIKSPKALNYLKSFQPFEIVSLNDLIVGQKAIA
                     IGNPFGLDHSLSKGVISALGRKVPGIGGVTIYDMIQTDTPINPGNSGGPLLNSAGQLI
                     GMNTMIYSRSGSSAGIGFAVPAEDIQKIASQIINHGRVVLSGIGIQRVEPHLAERLGV
                     KKGILIADVVPGTPADKLKLRGTHRDQWGRIVLGDVIVGVNAHPVPNYDALYNLLTEI
                     KVGEQITVSIIRHGKKMDVKMKTIDIAAL"
     gene            complement(3558..3896)
                     /locus_tag="I9760_002351"
     CDS             complement(3558..3896)
                     /locus_tag="I9760_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044110.1"
                     /translation="MVYIKRDKDGRICAVYNQKTSDVTEQIDINDAEFVDFLLRCESD
                     EKLKFLQSDLQLIRVLEDLIDVLIDKNIITITDFPQSVIDKLLARQAFRKRFASRAGM
                     ELNGDDETTT"
     gene            complement(3874..4056)
                     /locus_tag="I9760_002352"
     CDS             complement(3874..4056)
                     /locus_tag="I9760_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044111.1"
                     /translation="MLVLNDIPGIIHESLLSGFGLTINMEGLSLETADIRFMCLFFLN
                     IVLAKKGLNSGLHKTR"
     gene            complement(4112..5008)
                     /locus_tag="I9760_002353"
     CDS             complement(4112..5008)
                     /locus_tag="I9760_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044112.1"
                     /translation="MEPVTIALICAASFGAVVALSAFIRQLLLSRDKNLNDEAQRKAI
                     NQEAGELEKMREQMQSNKRFDSHYKVLGANKDAIIYLDNKIEDILRKKAHLVERYAQV
                     SLKESGAIVEGGISTERKDACDRLKKEIDEEIKFYDSELLHLQQRRTSLWDTHTDLQE
                     YLLGQEKSRNESLDHLYKQHTALLEKIYIRHTDDSENIAKQSIDAGTTSFKAIILAPI
                     QFLLQYFNISSGINFDQSKLENAARDDVDKTESDINDSENSDDEIYSDTDANSDDESN
                     RSDNEDSRDESDSENDSRLIFA"
     gene            complement(5019..5675)
                     /locus_tag="I9760_002354"
     CDS             complement(5019..5675)
                     /locus_tag="I9760_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044113.1"
                     /translation="MPQRQSEIVVLKPTNLFLSFLASQLPEANLPSLKLLHTDNTAYV
                     IPKHDSDDGTLNEIEKHFTTMFRHEICRWLGRSAHNEIETSFLDFLCCFKFELHSHII
                     LMEPSLKEGHQMLNIKPRSALLEWMKCAVEDQEGLSDVMSRVNLAQIAENSTVIVKNF
                     TTIKDVKPFIKQYFKPIFETTMSRISGQSVQWPQVNSFQSFSRYFAVEIHTQLIHLHY
                     "
     gene            6089..7630
                     /locus_tag="I9760_002355"
     CDS             6089..7630
                     /locus_tag="I9760_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAT5044114.1"
                     /translation="MKLLGKILLVFTALMIFVSITLWILATNIKPETIKQLVSNKITA
                     ITHKQSQIDGTISWQLFPRPGLKFNKIHIGNENLNDNYSLLIDTLLLNLKITPLLKGH
                     FVFSEINIDGLKLFINQENNEKPLSSEKNANQYNTTTYPNEQFAIQKLLLSHGQIILN
                     NKGHSTVLKNIQIGAEQFNLKNSPFSVQIKAKLTDAAFLQTAKANINFKGRVSLSPSI
                     IDELNSGISKSSIEGQLQIQNILLNQFAIKKINATLKTHKRDIQFNPLTLSLYNGESI
                     GDMDYVIATQQLLINQTATNLDGKQLITSLLKHPAISGNLDYSIHASIPLKALSIESL
                     VSKGTITLKDGEVYNINLDQLLNNLKVKLNSLMTETPDNIKKLTQSADWDQNKNMQGN
                     TKFKLANFKFQLQTGAISSDSFLLQTDKLQVNGEGRINLLNQEINSNIKATLNDNSTD
                     NTLQKIQQALGGYFPLVVSGTVENPIVLPDFKAISPVLSSLAIKSALTKPLKIIQKPL
                     KELIH"
     gene            7627..>8634
                     /gene="mutY"
                     /locus_tag="I9760_002356"
     CDS             7627..>8634
                     /gene="mutY"
                     /locus_tag="I9760_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAT5044115.1"
                     /translation="MSTNSLNQLFSQLLLDWYDLHGRKDLPWQLPRSPYRVWVSEIML
                     QQTQVQTVIPYFNRFIEHFPDIFLLANAEEDEVLSLWSGLGYYSRARNLHNTAKIISD
                     QYNGVFPEDLNILVQLPGIGPSTAAAILSQAFNKPAAILDGNVKRVLSRFFLIEGWPE
                     QAQVKKKLWGLASSCMPNERCADYTQAIMDLGATCCTNKNPQCLRCPVKNHCLAFHNK
                     KQHLYPTKKIKKQRPILSQQFLVLHNGQNQVYLEKRPPSGLWGGLWCLPSINNQTCPI
                     EHIQLFYKLQGDSPKLITRFKHSFSHFHLEITALSIRIEPMNNFISESRGQWFTKETL
                     PT"
BASE COUNT         2851 a         1754 c         1383 g         2646 t
ORIGIN      
        1 tcatatggtt aaccaaatta atgactctgc caatttaagg catatcgata tggacaatcg
       61 tatcaatgca aatcacaagg aagtcaaaag ccctgttctg gaaaataact ctactgatag
      121 cgtcaattta agtagtacat cgaagcaact ggaagcttta aaagcatctc ttaaagattt
      181 acctgaaatc aatgaggctc gtgtattgta tttcaaagct gaaattcaat ccgggcagta
      241 tgaaattgat agcagcaaga ttgcacatgg catgttaaat agtgtagaga tggtttaaac
      301 tgcagggcga cccgatgaat aataacaata aagcaaaaac attaataacc caattagaac
      361 aggaaattag ctgggtagaa gaactcaata ctttattatc tgaagaaaaa gagatacttt
      421 ctaccagtca attcaatcag ctggagacat tagctgagaa aaaacaggaa ttatcaacta
      481 aattagaggc tagtgcaaaa caacgtgtag atcttataaa agaatcaaat cccggcgaca
      541 aacctcatgg actcctgttg tctgattttc tcaaaaattg cgctacagaa gatggcattc
      601 aaatcaatca tttaaataat actcttgcag agaaattaaa cctatgtcgt gagttaaata
      661 ccattaatgg acaagtcata gcgaataaca tgtatacccg tcaacaaatt gtcaacgcct
      721 tgtcgggaaa taaatctaat gctgtgagtg tttacacttc taacgggaat ttggaaaccc
      781 cttccgaaag cagtcaccat gaagaagcat aagaaatata acccctagta ttttttaaaa
      841 tagctctcca gtatgatgcg ccaggcatag ccgctcaacg catacggaga gccgcatcca
      901 aacgaattac ctctccatta atcatctggt tttcaataat atgacctact agtgaagcaa
      961 attcctctgg tctgcccagg cgtttgggaa aagtcactgt tgcaaccaaa ctgtcctgaa
     1021 cttcttgagg catatttaaa agtaaaggag ttgctattaa ccccggcgca attgtattta
     1081 ctcttattgc aaattgagct aattcacgtg ctgctggcaa ggtcattgat actattcctc
     1141 cctttgacgc actataagca gactgtccta tttgtccctc aaaagcagca atggaagctg
     1201 tattgattat gacaccccgt tcctgagatt tgttgtctaa ctccaatcct gacatggcat
     1261 gtgcagctat tcgcatgacg ttgaaggtac ctattaaatt gacatctatt acttgtctaa
     1321 atgattccaa aggcattggc ccttcttttc ctaccatgcg ttttgccgga gcaattcccg
     1381 cacaattgat acaaaccctt ggtactccta cctgagagat agtttgtcgc attgcctttt
     1441 caacagattc atcactggtt acatcacaag caacatacaa ttccgcctca tttgaggtgc
     1501 cttcctgttt atcccagacc acaactttca ttccatgctg ttgtaagtat tgtacacaag
     1561 ccttacccat acctgagaca ccaccagtta ctagtgcaat ctgattatcg aaattcattt
     1621 tctctccaaa attctacggt ttcaatttcc aactttctta ttctacttaa aaacagtatt
     1681 ctattagacc tcttggcaaa cctgcttatt ttttaaggca aagcacaaaa ccgctttgag
     1741 gaaggaagct tacatacagt acatgagtac cgcagaaacg tttgcaacac agcaataaaa
     1801 ggaaataatc aggccatgca aatggtctat tgtaaaaatt ctttgctaat cgctttaaat
     1861 tctggcgtac ctgcttgttt taaccactcg aacagtacca tttcagcggt tatcaaatgg
     1921 atcccattct gtttcatcct ttttagaccg tattttaaat caaactcaga gcgactgctc
     1981 acaccatcta caacaacaaa gacctcgaat ccatagccct tcatttctat ggccgtttga
     2041 agcacacata catgagcctc aattccgata agaataagct ggtttttatt atatcctttc
     2101 aggaggttaa tatattgtgg ttcttgcatg catgagaaat gaattttttc aatgcattct
     2161 tcttgattga tataagagct taaaggagaa acggttcgac ctaaaccttt agggtattgt
     2221 tcactagcta gaacaggcac gtccagtttt ctggctaatt ttagtaacca ttcacatcga
     2281 gctaaaaaaa gatcgagatt taatattgcc ggggttagtt tttcctgtac atcaatcaag
     2341 accaaaagag aatccgctct atttagtaac atcctgatag cctcattcta tttattgatc
     2401 tatatcatat agcattaaga aaagtgagtg aaccccggat tgaggcataa ttcttttccg
     2461 gggaaataat agttagaaaa aatgaagaga gttaaagcgc tgcaatatca attgttttca
     2521 ttttgacatc cattttctta ccatgcctta ttattgaaac agtaatttgc tctcccacct
     2581 taatttccgt gagcaaatta tacagggcat cataatttgg gacaggatgt gcattcacac
     2641 ctacaataac atcccctaaa acaatacgcc cccattgatc tctgtgagta cctcgtaact
     2701 tcaatttgtc tgcaggagtt ccaggcacca catcagcaat caaaataccc tttttaactc
     2761 ccagcctttc cgcaagatgt ggctcaactc gttgaatacc gataccagac aataccactc
     2821 gaccatgatt aataatttga gatgcaattt tctgaatatc ttccgcggga actgcgaaac
     2881 caatacctgc agaagaacca gatcgtgaat aaatcatggt gttcatacca attaattggc
     2941 ctgcgctatt taatagagga cctcctgaat tacccggatt gataggggta tctgtctgaa
     3001 tcatatcata aattgttacc ccacctatac ctggtacttt tctacctaaa gcagaaatca
     3061 ctccctttga taaactatga tccaacccaa aaggattccc aatcgcaatc gctttttgcc
     3121 caacgatcag atcattaaga gagactattt caaagggctg aaatgatttc aaataattca
     3181 gagccttagg tgatttaatt tcaaggactg caatgtcctt gcgaggctct gaaccaatca
     3241 ctttggcagg gacagtcata tttcccaaag taattgctat gtcatcagca ccgttaatga
     3301 catgaaaatt ggttacaaca tggcctttgt tatcccaaat aataccggag cctgccccat
     3361 caggaatatg tgttttttgt aatgagtaac gacgctgaac agtagcattt gccaatcgat
     3421 gaacataaac aactttggat gaggcttttt gaaatacttc aactgtattt cgttcatctg
     3481 gaaggagagc atccaggttt aaactaaaaa caggtcttgc cagaataaaa cataaaataa
     3541 aagggataag taataagtta cgttgttgtc tcatcatctc catttaactc catacctgca
     3601 cggcttgcaa atctcttgcg aaatgcctgt cgcgctaata atttatcaat tactgattgt
     3661 ggaaaatctg taatagtaat gatattttta tctattaaaa catcaatcaa atcttcaagt
     3721 acacgaatca attgcaaatc ggattgtaga aattttaatt tttcgtcgct ctcacaacgc
     3781 aacaaaaaat caacaaattc tgcatcatta atgtcaattt gctccgttac atctgaggtc
     3841 ttctggttat atactgcaca aatccgacca tccttatctc gttttatgta gaccactgtt
     3901 taatcctttt ttagctaata ctatgtttag aaaaaacaga cacataaatc ttatgtctgc
     3961 tgtctctaaa ctcaaacctt ccatgttaat ggtaagccca aacccagaca aaagactttc
     4021 atgaattatt ccaggaatat cattaagtac aagcattgta ttgtaatata tcctttcata
     4081 gatgtcacca attaactcaa aagattaatt tttatgcaaa aattagtctt gaatcatttt
     4141 cagaatccga ctcatcccga gagtcttcat tatctgaacg attagactca tcgtcagaat
     4201 tggcatcggt atcgctataa atctcatcat cgctattctc agaatcattg atatcacttt
     4261 cagttttatc aacatcatct cgcgcagcat tctccaattt agattggtca aaattaatac
     4321 ctgatgaaat attaaaatat tgcagcaaga attgtatagg cgccaaaata atcgccttaa
     4381 atgaggtggt tcctgcatca atactttgtt ttgcaatatt ttcactgtca tcagtatgcc
     4441 gaatatatat tttttctaaa agtgctgtat gttgcttgta gagatgatcc agactctcat
     4501 tgcgagactt ttcctgaccc aacaaatatt cttgtaaatc cgtatgagta tcccacaatg
     4561 aagttcgcct ttgttgcagg tgcagcaatt cactatcata gaattttatt tcttcatcta
     4621 tctccttctt taacctgtca caggcatcct ttctttctgt cgagatccca ccctcaacta
     4681 tggcccctga ttccttgaga gatacttgtg cataacgctc cactaagtga gcttttttac
     4741 gtaaaatatc ctcaatttta ttatccaggt agataatcgc atctttattc gcgccaagaa
     4801 ctttataatg agaatcaaat cgtttattgc tttgcatctg ctctctcatt ttctcgagtt
     4861 caccggcctc ctgatttatt gcttttctct gtgcttcatc atttaaattt ttatctctac
     4921 ttaataatag ctgtctgata aatgcggata acgctacaac tgctccaaac gacgccgcac
     4981 agatgagggc tatagtcact ggttccatag tcaactcctt aatagtgcaa atgtatcaat
     5041 tgggtgtgta tttcaacagc aaaataccgg ctgaaagatt gaaatgaatt gacttgtggc
     5101 cattgtactg attgaccgct tatccggctc atagtcgttt caaatattgg cttaaaatac
     5161 tgttttataa atggctttac atctttgata gttgtgaagt ttttaacaat aaccgttgag
     5221 ttttccgcta tctgagccaa attcacccga ctcataacat cagaaaggcc ttcttgatct
     5281 tcaacagcac atttcatcca ctctaaaagc gctgaacgag gcttgatatt caacatctgg
     5341 tgcccttctt ttaaagaggg ttccattaaa ataatatgag aatgtaactc aaatttaaaa
     5401 caacacagaa aatcaagaaa acttgtttca atttcattat gtgcagaacg ccccaaccaa
     5461 cgacaaattt catgtctgaa cattgtagta aaatgctttt cgatctcgtt aagagtacca
     5521 tcgtcagaat catgcttggg aataacataa gccgtattgt cagtatgcaa taattttaag
     5581 ctgggtaaat tagcttccgg taattgtgac gctagaaagg ataaaaataa attagtaggc
     5641 ttcaacacta ctatctcaga ttgtctttga ggcatgaggc tctccttgat ttaattaact
     5701 cctcctgatt acatctcaac tattaaaatc aacgtctcat ctcaatttag aatcttagtc
     5761 actatcttga ctaatacaga gttttattca caaatgtgca tgaagtcaca cttgactttc
     5821 aaagacttaa aaattccctg atattgttaa atttagaacc aagattaagt acatcaacca
     5881 aaattctggt aatacattaa ctcaaaagtc caaaaaaaaa tctctcttat tcagtctaat
     5941 ttgcatatat gatagtttca atagttaaac agaatttttt tgagaatatt tttccaattt
     6001 gttagaatag acacctatag tcttaaatcc ttcttgacag aaactccttt atctatatgc
     6061 tttggttttt aaactctggt tctatcccat gaaattgcta ggaaaaattc tactcgtttt
     6121 tactgcttta atgatctttg tatcaatcac tttatggatt ttagcaacaa atattaaacc
     6181 cgaaaccatt aagcaattgg ttagtaataa aatcacagca atcactcata agcaaagcca
     6241 aattgatggg actatttcct ggcaattgtt cccaaggcct gggctcaaat tcaacaagat
     6301 acatataggt aatgaaaatt taaatgataa ttattctctt ctgattgata cattgctatt
     6361 aaatttaaaa attactcctt tgttgaaagg acattttgta tttagtgaaa ttaacattga
     6421 tggcttaaaa ttatttatca atcaagaaaa taatgaaaag cctctctctt ctgaaaaaaa
     6481 cgctaatcaa tacaacacca caacgtaccc caatgagcaa tttgccattc aaaaattatt
     6541 attaagccat ggacaaatta tccttaacaa taaggggcat tctactgtct taaaaaatat
     6601 tcaaatcgga gcagagcaat ttaatttaaa aaactcgccc ttttcagtac agattaaagc
     6661 caaactgact gatgctgctt ttttacagac agccaaagct aatattaatt ttaaaggtcg
     6721 agttagtcta tcaccctcaa ttattgatga actcaattca ggaattagta agtcttccat
     6781 agaaggccaa ctgcaaatac aaaatatttt attgaatcaa ttcgctatca aaaaaatcaa
     6841 tgccactctt aaaactcata aaagagatat ccaatttaat ccacttactt tgtcactcta
     6901 taatggggaa tcaataggag atatggacta tgtcatcgct acacaacaat tgttaattaa
     6961 tcaaactgca accaatttag atggaaaaca attaatcact tctttattaa aacatcctgc
     7021 aattagcggt aatttagatt actcaattca tgcctcaata ccattgaaag cactcagtat
     7081 tgagagtctt gttagtaaag gaaccattac gcttaaagat ggagaagtct ataatattaa
     7141 tcttgatcaa ttacttaata atctaaaagt aaaacttaat tcgcttatga ctgaaacgcc
     7201 agataacata aaaaaactga ctcaatctgc ggattgggat caaaataaaa acatgcaagg
     7261 caataccaaa tttaaattag caaattttaa attccagtta caaaccggag caattagcag
     7321 tgattcattt ttgctgcaga ctgacaaact ccaggtgaat ggagaaggga gaattaatct
     7381 gctaaatcaa gaaataaact ccaatattaa ggccacgtta aacgacaaca gtacagacaa
     7441 tactttgcaa aaaattcaac aagcattggg tggctatttc ccactggtag tatctggcac
     7501 agttgaaaat cccatagttt tgccagattt taaagcaatt agccctgtat taagttcgtt
     7561 agcgatcaaa tcggctctga caaaaccttt aaaaataata caaaaaccac ttaaagagct
     7621 aatccattga gtacaaactc tttaaatcaa cttttcagtc aattactgct tgattggtat
     7681 gacctgcatg gacgcaaaga tttgccatgg caattacccc gatctcccta cagagtttgg
     7741 gtatcagaaa tcatgttaca acaaactcaa gttcaaactg ttattcccta ctttaatcgt
     7801 tttatagaac atttccctga tatttttttg cttgctaatg cagaagagga tgaagtgcta
     7861 tctctatggt cggggttagg ctattacagt cgcgccagaa atcttcacaa cacagcaaaa
     7921 ataattagtg accagtataa tggcgtattt cctgaagatc tgaacatttt agttcaactg
     7981 ccaggaatag gaccatcgac agcagcagcg atactttcac aagcttttaa taaaccagct
     8041 gctattttag atgggaatgt gaaacgagtt ttatctcgat tttttttaat cgaaggttgg
     8101 ccagaacaag ctcaggttaa aaaaaaacta tgggggcttg cgtcttcttg tatgcccaat
     8161 gaacgatgtg cggattatac acaagcaatt atggacttag gtgcaacatg ctgcacaaat
     8221 aaaaatcctc agtgtttgcg ttgtcctgtt aaaaatcatt gcctggcctt tcataataaa
     8281 aagcaacatc tttatccaac aaaaaaaata aagaaacagc gcccaatatt atcccaacag
     8341 ttcttggtgt tacataatgg acaaaatcag gtttatctgg aaaaaagacc tccctcaggg
     8401 ttatggggag gcttatggtg tttacccagt ataaataatc aaacttgccc aattgagcat
     8461 atccaactct tttacaagtt gcaaggagat agcccaaagc taattacgcg gtttaaacac
     8521 agcttcagcc attttcattt ggaaataaca gcattgagca taagaataga gcctatgaat
     8581 aattttatct ctgaatccag aggacaatgg tttacaaaag aaaccttgcc aaca
//
LOCUS       DACTJE010000141         8515 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.141, whole genome shotgun sequence.
ACCESSION   DACTJE010000141 DACTJE010000000
VERSION     DACTJE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.141"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            103..1572
                     /locus_tag="I9760_002357"
     CDS             103..1572
                     /locus_tag="I9760_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009649652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HAT5044116.1"
                     /translation="MLFNSFGFIFVFLPIVLCVFYFFLQFNLQKFTILWLVAASLIFY
                     AQWNINYLGLLLASICFNYFISTKILYFQKNKNSCAAKKYLLLGVFADLMLLGYYKYT
                     NFGIEALNTAFHWHISSQNIILPLGISFFTFTQIAYIVDAFKLKVTEYSFSKYCLFVT
                     YFPHLIAGPILHHGEMIPQFSLSKLKNNKIFQNIYFGLLIFIVGLFKKIMIADPLSLY
                     VAQGFDQAYSLSFLDAWITSLSYTFQLFFDFSGYSDMALGLGLMFQTYLPINFNCPYR
                     AINIQDFWRRWHITLSNFLRDYIYIPLGGNRGKQGRLYGNIIITFLIGGLWHGAGWLF
                     LIWGGLHGIALVIHRQFQKFHHQLGNIQSWFITFLFVNFAWVFFRANSLDSATKIIKG
                     MFGANGFTSAYSLFNLNTLLDKYKVAYDLLPFLNTNQFAYLCLIVSMVFASILTVQST
                     HNFVKKIKPNFIMLLLILIATILLLFNLNKSTEFLYFNF"
     gene            1588..2907
                     /locus_tag="I9760_002358"
     CDS             1588..2907
                     /locus_tag="I9760_002358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044117.1"
                     /translation="MLDNLGFHQVEENEKKYKWFVIGYLVVLSTPIVLLLFSYLFLIN
                     AFELRNFTDIVKKQNEKNAIYGSGVDVYYYDYKREFVKQRKPVIMALGSSRALSFHEE
                     VFTVPFTNAGQGMVSLEQGIVFIRDMLQYHKPKAIILSLDFWWFNDRVGGKGYGFTTF
                     DGTKVTLTKLLKPYTLLAEGNISWKDFLTITFLAKKNNSLTNFNNLGILAIKTSSGTK
                     SDGSIHWGHSVLTGEDVYDYKFQRGLKYIEDGVSNYEYAQDFSKSRMEELDQIVKLCK
                     DNKIELFIFIPPVAETIYLKMASMRDKYLYIDKLRNYLHNYPVYVNDFHDPDTIHSSN
                     CEFPDHYHGGDVAYLKILLQMAENPKNDGLKKYLKMDLIREMINKFEGNVLVQFDESM
                     YKVQEKDMHNFGCRKQATCIANKAAVLPQLFSKYIITDKFDCLRSEG"
     gene            2904..3779
                     /locus_tag="I9760_002359"
     CDS             2904..3779
                     /locus_tag="I9760_002359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044118.1"
                     /translation="MKFKELMAQLKNGNTISDHANEVGRKNWSEAVKAANFPPSNLRK
                     EFLKKGYISLGNSILPSELIKELQEEFRAVIQDDVHSTYSFDDPILLNAYNHGGYKGE
                     VDKFRRDIIDCAKSLPSTSKIWANETFRNVIEELIGSPFQEDGMAAWRINHVPEHIYE
                     KFEATTNRFHFDSQYVDRIKIFIFLTDVTLENGPFQHFSRRYSRYLILRGFREEKRRV
                     SITSGLPKNIINSPRLKKHTGPAGHILVCATSFCIHRAGEVMPGHHRDMLQLCVRPRV
                     ISSNETPEKSDVRML"
     gene            complement(3920..4903)
                     /locus_tag="I9760_002360"
     CDS             complement(3920..4903)
                     /locus_tag="I9760_002360"
                     /inference="COORDINATES: protein motif:HMM:NF017532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044119.1"
                     /translation="MNSGDLELQDLKLDGYIVLSGIISADEIEQARQEFEKWFQQDIE
                     SRQQNNINDPHYPNGPFGYTILTEASHLALNVYSKSECFDKLVDKVLSHDKIQQIIKA
                     WNGPGYKVSGINLRFMTGAFDPPPAHELHRDHPGSMNLGIMLTDVEPGNNGATALVPG
                     SQWSNVDPRWDCLFQKPFRLKKDPSKNGIDLFLKINIFNFLFKKKSLKKMTGAFGKKG
                     DVYFFANGEVWHGRLPNLHGQRAMICLIGVHPVDEESINTGSQIPPEILAKLPSNLAK
                     NLNGPFEINNPENSIREHIYSNRKRLSIFSLAYWSKLERRLAEWISARKIK"
     gene            5080..6621
                     /locus_tag="I9760_002361"
     CDS             5080..6621
                     /locus_tag="I9760_002361"
                     /inference="COORDINATES: protein motif:HMM:NF015049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HAT5044120.1"
                     /translation="MLFTSQLFIFFFLPLTCCGVFFSHRFFGKQGSILAIIGFSFAFY
                     YLNGQSQLPLLLTSIAVNHIFIKIIVATKDANRKNLLTGLAVLLNILALIYYKYGIFL
                     VKQYESLKLVIDQHIPLNAWSTYIDGYNLIYPWGYSGEMIILPLGISFFTFQQIAYIL
                     DLRSGRVKNHNFLDYLFCVTFFPHLIAGPIVNYRELIPQLKNKKILIFKNTNILIGLA
                     IFSFGLFKKAIIADTLAEIANPVFDGLTHGANINFISAWLSAIAYTLQIYFDFSGYSD
                     MAIGLARMFGLKLPVNFASPYKATSIIEFWQRWHITLSRFLKNYLYIPLGGNRYGQLL
                     KYRNLLLTMTIGGIWHGAGWTFVIWGVLHGVLLSINHLWREQCNKFPKLKNVIPSSIS
                     RILTLLAVIILWVFFRASSVDSATRILAAMISPDFDQGFIVFSWKYSLILIAGYISLC
                     LPSTQQLFSRYYTAIPPEGLPGVITGGALTWRINIPWLLVTTVLAAVACFYSTDFPQF
                     LYWNF"
     gene            6625..7674
                     /locus_tag="I9760_002362"
     CDS             6625..7674
                     /locus_tag="I9760_002362"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044121.1"
                     /translation="MKSLNKKSAYGQYTSLVIFLLILVFLFVGAITAYFSNGYLSPLW
                     QIAKCNWKPPGENYVMAGCDPIISPFYRLGSLYLDVEPKLTEALRKADVVITGNSRTL
                     YTFTVKLRNNPIEEYFRKKNLKAFIVAEEGSGFRFRKMVLEKLKIHPKIALINTEDLA
                     ADLLEDDNRELIFNQDRFKLPFKIIHFSIEMQYSICTSKKNNLIMRKLKNFYCNGSIT
                     PNWRSLSTGVVAQTNKRIPTNRQLITMTSDTHIDSIDIFRRRMHTMFKSKAWRDSCVI
                     FYEIPNPNQFIEVSTQLAREAKKPFIFPSITPEKNYFVFDGSHMEKDTAIRWTNEFLQ
                     ILEPYLNNCLGKNYA"
     gene            complement(7805..>8515)
                     /locus_tag="I9760_002363"
     CDS             complement(7805..>8515)
                     /locus_tag="I9760_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT5044122.1"
                     /translation="KNHANLGFSAANNAVYPMIETPFILYLNPDCFIGENTIEHLLQV
                     MDNYPDAGMAGCLVTNPDGTEQAGCRGLTPTPARVFNQMLKLEKFFPNNSKFSGYLLS
                     DKPLPDRPQEVELISGSCMFVRKKTIDDIGLLDAKYFLYCEDYDWFFRVIQGGWKIIF
                     TPQTKVTHIKSYSTQQIPIRVLGYKAKGMWRYHSKFFKKTSPTIMTLLVRLGIISRLL
                     VLSSITLFKKSYKKLLNY"
BASE COUNT         2678 a         1296 c         1476 g         3065 t
ORIGIN      
        1 tttaatggaa aataagaaaa aatagaaatt aaaacatttc aatggtattc tccaaaagcc
       61 aaaaaacatg gaaacataac tatttttaga tgcggaagca atatgttatt taattctttt
      121 ggatttattt ttgttttttt acctattgtt ctgtgcgttt tttatttttt tcttcaattt
      181 aacttgcaga aatttactat tctttggttg gtagcagcct cattaatttt ttatgcccag
      241 tggaatatta attatcttgg attattattg gcttcaattt gttttaatta ttttataagt
      301 accaagatat tgtattttca gaaaaataaa aattcttgtg cagcaaaaaa atacttatta
      361 ctgggagttt ttgctgatct tatgttacta ggatattata agtacactaa tttcggtatt
      421 gaggcattaa atacggcttt tcattggcat atttcatctc aaaatattat tttaccttta
      481 ggtatttctt tttttacctt tactcaaatt gcatatattg tagatgcttt taagctaaaa
      541 gtgactgaat atagtttttc aaaatattgt ctttttgtga cctatttccc acatcttatt
      601 gcgggcccaa tattgcatca tggggaaatg atacctcaat ttagcctgag taaactcaag
      661 aataacaaaa ttttccaaaa tatatatttt ggattgctta tttttattgt gggattattt
      721 aaaaaaataa tgatagcaga tccgctttca ttgtatgttg ctcaaggatt tgatcaggct
      781 tatagtctat cctttctcga cgcgtggatt accagcctgt catatacttt tcagctattt
      841 tttgactttt ccggttattc agatatggca ttgggacttg gtttaatgtt tcaaacgtat
      901 ttgcctatta attttaattg tccctaccgt gccataaata ttcaggattt ttggcggaga
      961 tggcacatca cactatctaa ttttttacga gactatattt atattccatt aggggggaat
     1021 agaggtaagc aaggtcgtct ctatggcaat ataataatta catttcttat cggagggctt
     1081 tggcatggtg ctggttggtt atttttgatt tggggcggat tgcatgggat tgctttagtt
     1141 attcatcgac aatttcaaaa atttcaccat caattaggaa atattcaatc atggtttatt
     1201 acatttcttt ttgtcaattt tgcatgggtt ttttttaggg ctaattctct tgattctgca
     1261 acaaaaataa ttaaaggcat gtttggtgca aatggtttta cttctgcata tagcctattt
     1321 aatttaaata ctttattaga taaatataaa gttgcctatg atctattgcc atttttaaat
     1381 acaaatcagt ttgcctattt gtgtcttatt gtttcaatgg tttttgcatc tattttaacg
     1441 gttcagagta cacataattt tgttaaaaaa ataaaaccga atttcattat gcttctatta
     1501 attttaattg ctactatatt gctactcttt aatttgaata aatcaacaga atttctttat
     1561 tttaattttt aacttggtac caacataatg cttgacaatc ttggatttca tcaggtagaa
     1621 gaaaatgaaa aaaaatataa gtggtttgta attggttatt tggttgtttt atcaacacct
     1681 attgtgttgc ttttgtttag ttatttattt ttgattaatg ctttcgaact taggaatttt
     1741 acagatattg ttaaaaaaca aaatgaaaaa aatgcaattt atggttcagg cgttgatgta
     1801 tattattacg attataagag ggaatttgta aagcaaagaa aaccagtcat tatggctctt
     1861 ggctcctcaa gagctttgag ttttcatgaa gaggttttta ctgtaccatt cactaatgct
     1921 ggccagggca tggtttcact ggagcaaggg attgttttta ttagagatat gctgcaatat
     1981 cacaaaccaa aagccattat tcttagtctt gatttctggt ggttcaatga tagagttggt
     2041 ggaaaaggat atggttttac aacatttgat ggaacaaaag ttacattaac aaagcttctt
     2101 aagccctata ccttgctagc tgaaggaaat atttcttgga aagatttttt aactattact
     2161 tttttggcaa aaaaaaacaa ttctttgact aactttaata acttaggcat tttagcgatc
     2221 aaaacatctt caggcactaa aagcgatggt tcaattcatt gggggcattc tgttttaaca
     2281 ggcgaagatg tatatgatta taaatttcag agaggattga aatacataga agatggtgtg
     2341 agtaattatg aatatgcgca agatttttct aaaagtcgta tggaagaatt agatcaaata
     2401 gtaaaattat gtaaagacaa taaaatagag ctttttattt ttattcctcc agtagctgaa
     2461 accatatatt taaaaatggc atcaatgagg gataaatatc tctatattga taagttgcgt
     2521 aattatctac ataattatcc agtttatgtc aatgattttc atgatccgga taccatacat
     2581 tcatctaatt gtgagtttcc agatcattat catggtggtg atgttgctta tttaaaaatt
     2641 ctattgcaaa tggctgaaaa ccccaaaaat gacgggctaa aaaaatatct taaaatggat
     2701 ttaataagag aaatgataaa taaatttgag ggtaatgttc tggtgcaatt tgatgagtca
     2761 atgtataaag tccaggaaaa agatatgcat aattttggtt gtagaaagca ggcaacctgt
     2821 attgccaaca aagcagcagt cttacctcaa ctgtttagta aatacattat aacggataaa
     2881 tttgattgtt taaggagtga agggtgaaat tcaaagagtt aatggctcaa ttaaagaatg
     2941 gaaatacgat tagcgaccat gcaaacgaag ttggaaggaa aaattggagt gaagcagtca
     3001 aagcagccaa ttttcctccc tctaatttac gaaaagaatt tttaaaaaag ggttatatat
     3061 ccttaggaaa ttcaatattg ccatctgaat taatcaagga actgcaagaa gaatttcgtg
     3121 ccgtgatcca ggatgatgtt cattctacat attcattcga cgatcctatc cttttaaatg
     3181 catacaacca tggtggttat aaaggtgaag tagataagtt caggcgagat atcattgatt
     3241 gtgctaagtc acttccatca acctcaaaaa tatgggcaaa tgagacattt agaaatgtga
     3301 tagaggagtt gattggctct ccttttcagg aagatggaat ggctgcctgg cgtataaatc
     3361 atgttccgga acatatctac gaaaaatttg aagctacaac aaaccgattt cactttgata
     3421 gtcagtatgt tgatagaatt aagattttta tttttcttac tgatgttaca ttagaaaatg
     3481 gtccattcca acatttttca agaagatatt cacgctatct tatattgaga ggatttcgtg
     3541 aggaaaagag aagagtttca attactagcg gcttaccaaa aaatattatc aattcgccac
     3601 ggcttaaaaa acatacggga cctgcaggac atattttagt gtgtgccact tctttctgta
     3661 ttcatcgtgc tggagaagtt atgcctggcc accatcgtga tatgttgcag ttatgtgttc
     3721 ggccacgtgt aatttcttct aatgaaactc cggaaaaatc cgatgttagg atgctataat
     3781 ttttaggaag tgcgatgttt ttcaaaataa aatacaatcg aaaatctatt gaatttcgat
     3841 gattttgcta ttatttgggg ctcaactgtt taatgagttg atcggacgtt gtagccccaa
     3901 taatcaatcg acaaattaat tatttaattt ttctagcaga aatccattcg gccaatcggc
     3961 gctcaagttt agaccaatat gcaaggctaa aaattgataa ccgcttgcga tttgaataaa
     4021 tatgctcacg tatagagttt tctgggttgt taatttcaaa tggtccgttt aggtttttag
     4081 caagatttga tggaagtttt gcaagaattt ctggggggat ttgggaccct gtatttattg
     4141 attcttcatc taccggatga acacctataa gacaaatcat cgctctttgc ccgtgaaggt
     4201 ttggtagcct cccatgccaa acctctccat ttgcaaaaaa atatacatct ccttttttac
     4261 caaaagctcc agtcattttt tttaatgact ttttcttaaa taagaagtta aaaatgttaa
     4321 ttttaagaaa taaatcaata ccatttttag atggatcttt ttttaggcga aatggttttt
     4381 gaaagaggca atcccaacgg ggatcaacgt ttgaccattg tgagccaggt actaatgcag
     4441 tagccccatt attacctggt tctacatcag tcaacataat tccaaggttc atgcttcctg
     4501 gatgatctct gtgtaattca tgagctggcg gaggatcaaa tgctcctgtc ataaatcgta
     4561 gattgatgcc acttacctta taaccaggtc cattccatgc tttaattatt tgttgaattt
     4621 tatcatgact aagtaccttg tcgacaagct tatcaaagca ttcactttta ctatatacgt
     4681 tcaaagccag atgactcgct tcggttaaaa tagtatatcc aaatggacca ttcggatagt
     4741 gtgggtcatt aatattattt tgttgtctgg attcaatatc ttgttgaaac catttttcga
     4801 attcctggcg agcctgttca atctcatctg ctgatataat accacttaaa acaatataac
     4861 catcaagctt aagatcttgt agttctaaat ctcctgagtt cattggtatc cctgaaaatt
     4921 ttagttactc attctacatt tttgttgatt ctttcgcaat gaaatctctg acggtacaag
     4981 acataagccg aaataataaa tttgagttca aaatataatt tattgctttt ggaagtagtt
     5041 tgtatatcat gatcaagcaa aatttattgg attgttaata tgttatttac atcccagtta
     5101 tttattttct tttttcttcc actaacatgt tgtggagtgt ttttttctca tagatttttt
     5161 gggaaacagg gtagcatttt agcgattata ggattttcat ttgcatttta ttatttgaat
     5221 ggtcagtcac aactcccttt acttctaacc tctattgcag tgaatcatat attcataaag
     5281 attatagtcg ctaccaaaga tgcaaataga aaaaatctct tgacaggatt agcagtatta
     5341 ctaaatattc ttgctcttat ttattacaaa tatggaattt ttttagttaa acagtatgaa
     5401 agtttaaaat tggtaataga tcaacatatt cctttaaatg catggagtac atatatagat
     5461 ggatataacc taatttatcc atgggggtat tctggtgaga tgattattct tcccttagga
     5521 atttcctttt ttacttttca gcagatcgcc tatattcttg atctccggag tggaagggta
     5581 aaaaatcata attttttgga ttacttattc tgcgttactt tttttccaca tctaattgct
     5641 ggtccaatcg taaattatcg agaactaata ccacaactta aaaataaaaa gattctaatt
     5701 tttaaaaata ctaatatttt gattggtctt gccatttttt catttggctt atttaaaaaa
     5761 gctattattg cagacacctt ggctgagatt gccaatccag tttttgatgg attgacccat
     5821 ggagcaaata ttaattttat tagcgcttgg ctatcagcaa ttgcatatac attgcaaatc
     5881 tattttgatt tttcaggtta ttctgatatg gcaattgggt tggcaagaat gtttgggtta
     5941 aagttacctg ttaatttcgc ttcaccatac aaagctacga gtattatcga attttggcaa
     6001 cgttggcata tcactctttc tcgtttcctg aaaaattatc tgtatatacc tttgggaggt
     6061 aatcgatatg gtcaattgtt aaaatatagg aatcttctac taacgatgac aattggtgga
     6121 atatggcatg gcgcggggtg gacatttgtt atttggggcg tattgcacgg ggttttgctc
     6181 tctattaatc atttatggcg cgagcaatgc aataaatttc ccaaattaaa gaatgttatt
     6241 ccatcttcga taagtcgaat cttaacactt ttggcagtga ttattttatg ggtatttttc
     6301 cgagcttctt cagttgattc agctacaaga atattagctg ctatgatttc tccagatttt
     6361 gaccaaggtt ttatagtttt ttcttggaaa tattctttaa ttttgatagc gggatatata
     6421 tctttgtgtc tcccttctac gcaacaatta ttttctcgtt attacactgc tattcccccc
     6481 gaaggtcttc ctggtgtgat tactggaggt gcgttaacat ggagaatcaa tataccatgg
     6541 cttcttgtaa ccactgtatt agctgctgtt gcttgctttt attcaacaga ttttccgcaa
     6601 tttttatatt ggaattttta agtaatgaaa tctctaaata agaaatctgc ttatggtcag
     6661 tatactagtt tagtaatatt tcttcttatt ttggtatttc tatttgttgg agcaataact
     6721 gcatactttt ctaatggata tttgtctcct ttgtggcaaa ttgctaaatg caactggaaa
     6781 ccacccggag aaaattatgt aatggctgga tgcgacccaa ttatttctcc tttttatagg
     6841 ttaggttctc tatatttgga tgtggagcca aaattaacag aagcactgcg taaagcggat
     6901 gttgttataa ctggtaatag tcgaacatta tatactttca cagtcaagct tagaaataat
     6961 cctatcgagg agtatttcag aaaaaaaaat ttgaaggctt tcatcgttgc agaagaaggt
     7021 agtgggtttc gttttagaaa aatggtgctg gaaaaactta agatccatcc taaaattgct
     7081 ttaataaata cagaggattt agctgcagat cttttagagg atgataatag agaattaata
     7141 tttaatcagg atcgttttaa attgcctttc aaaatcattc atttttcaat tgaaatgcag
     7201 tattcgatat gtacgtctaa aaaaaacaat ttgatcatga ggaaattaaa aaatttttac
     7261 tgtaacggat caattacccc aaattggaga agcctaagta caggagttgt tgctcaaacg
     7321 aataaaagaa tccctacaaa tcgacaatta attacaatga cttctgatac tcatatagat
     7381 tcgatagaca tatttagaag aagaatgcat accatgttta aaagtaaggc atggagagat
     7441 tcatgtgtga ttttttatga aatccctaat cctaatcagt ttattgaggt atcaacccaa
     7501 ttggcacgag aagcgaaaaa accatttata tttcctagca taactcctga aaaaaattat
     7561 tttgttttcg atggttctca tatggaaaaa gatactgcaa ttcgatggac aaatgaattt
     7621 ttgcagattt tggagcctta tttaaacaat tgcttaggaa aaaattatgc ttaatgtgat
     7681 aattaaaagt taaacttaga actaatatat tgagcaccag gaaagctttc ttttttctat
     7741 aattaccgga ttaatattga aggcaaaaca aataaattat gttaattttt aataaatggg
     7801 ctatttagta atttaataat tttttataag attttttgaa taaagtaata ctactcaata
     7861 ccaacaacct actaattatt cctaacctca ctaataaagt catgatggtg ggggaagtct
     7921 ttttaaaaaa tttactatga tatctccaca ttcccttagc tttataccct aatactctaa
     7981 tgggaatttg ttgagtgctg tagcttttta tatgagtaac ttttgtttgg ggagtaaaaa
     8041 taattttcca tcctccttga atcactctaa aaaaccaatc gtaatcttcg caataaagga
     8101 agtatttcgc atcaagaaga cctatatcat ctatagtctt ttttctaaca aacatacagg
     8161 atcctgaaat caattctact tcttgaggac gatctggcaa aggtttatca gagagcaagt
     8221 accctgaaaa tttggaatta tttggaaaaa atttttctaa cttgagcatt tgattaaata
     8281 ctctggcagg ggttggtgta agtcctctgc atcctgcttg ttctgttccg tctggattgg
     8341 ttacgaggca acctgccatt cctgcatccg gataattatc catcacttgg agcaggtgct
     8401 caatggtgtt ctcgccaata aaacaatctg gatttaaata aagtatgaag ggtgtctcta
     8461 tcataggata tacagcatta ttcgctgccg agaaaccaag attggcatgg ttttt
//
LOCUS       DACTJE010000142         8493 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.142, whole genome shotgun sequence.
ACCESSION   DACTJE010000142 DACTJE010000000
VERSION     DACTJE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.142"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(73..1002)
                     /locus_tag="I9760_002364"
     CDS             complement(73..1002)
                     /locus_tag="I9760_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAT5044123.1"
                     /translation="MDKPNDRTLHLKPTVRGGGIVFIGLSLLILPFIWYFTGSFYKES
                     VFLLLSILLIAGVSFFDDIYDLPIKIRLGVQCIVAFILAVFIRPEKLDFILFSMSHDY
                     IIIPFVFFMVIWAINHFNFMDGLDGFCASQAIYLLACYFILFNVHHALVYQEFCWILI
                     CSLVGFLIFNFPPARLFMGDIGSASLGLIIFSMALIAQQKYQIPVIYWFMLNSLFLFD
                     STITLLRRIVNKERWFAPHRKHAYQRLKQFGMDTRLILLAQLLMNGLIGCLVLLSWTQ
                     KVTILCALLMLTLILFSYYFIVERIYPMHFQAS"
     gene            complement(1073..2029)
                     /locus_tag="I9760_002365"
     CDS             complement(1073..2029)
                     /locus_tag="I9760_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAT5044124.1"
                     /translation="MTKVLVTGATGFIGSSLVPALIAAGYEVRCAVWQKNQYMSVEQV
                     EIDKLEQVTDWSEALNGIDIVIHLAARVHVMREDTSSSLEEYCKINSIATKNFAQQAA
                     KHKVKRFVFLSTIKVNGEVSLPQSPFSEKNSAQPKDSYAQSKLEAELYLREISENSDM
                     EVVILRPPLVYGPGVKANFLKLLGMVQKGWPLPFASIKNKRSFIFIDNLVSAILMVMT
                     HPKAANQLYLVADNESWSLADLLSLLAQNMKINLRLYHFPTTFLAGLFKITGMGSLNT
                     RLLSSLEIDNSKIISDLGWTPPVDSCEGLKKTADWYQYEYNA"
     gene            complement(2047..2922)
                     /gene="rfbA"
                     /locus_tag="I9760_002366"
     CDS             complement(2047..2922)
                     /gene="rfbA"
                     /locus_tag="I9760_002366"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAT5044125.1"
                     /translation="MKGIILAGGFGTRLYPLTKSISKHMIPVYDKPMIYYAISTLMLA
                     NIREILIISTEEHLPLYQNLLSDGSQWGISFAYLVQNEPRGIAEAFILGEKFIGNDSV
                     CLVLGDNIHQGRGFSELLQNAKSKLNGATVFAYYVDKPQAYGVVEFNEKQQVLSIVEK
                     PAQPKSNYAVTGLYFYDNQVIEIAKSLKPSGRGELEITDINQFYLYENKLDVQILGRG
                     FVWLDMGTPETLLSASNYICTIEQRQGLKIGCPEEIAWRMKFISDDELLCRARELNKS
                     SYGQYLAELLNHKRF"
     gene            complement(3077..4561)
                     /gene="pgi"
                     /locus_tag="I9760_002367"
     CDS             complement(3077..4561)
                     /gene="pgi"
                     /locus_tag="I9760_002367"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAT5044126.1"
                     /translation="MKRQTELPMWNLLRQEADKIRLTSGSQLTPFMNGMHLDYCKQIN
                     TIEYDYSRQKVNNTILDLLINLANEIKLQEKIDGLIRGEKVNISENRPALHTALRDLS
                     NKSILIDGLDIMSEVVSTREKIRVISNRIREKKWLGHSGLPITDIVNIGIGGSDLGSR
                     VCINALSNYISKEFNYHFISDVDPASFNDVIAKINPQTTLFIVSSKSFTTKETLLNAR
                     KAFALYEDTASIDQHFIAVTAHPERAYQMGIKTVLPIWDWVGGRFSFCSAVNLITAIA
                     IGYEQFVELLAGAHDIDTHVQFTDFKNNIPVLMALIGIWNNNFLNIHNLLILTYSKKL
                     EYFVPYVQQLDMESNGKSIDVSGRMVDYATGPIVWGGLGNQAQHSYFQLLCQGTHRCV
                     GDFITLKTNDEHEINSMCHYKMKVLSEGIQTIENPYGYIPGNMPMNHLILSDCSPYTL
                     GALVALYEHKIFVQSVIWNINPFDQPGIESAKSAHREITLSSES"
     gene            complement(4797..5702)
                     /locus_tag="I9760_002368"
     CDS             complement(4797..5702)
                     /locus_tag="I9760_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044127.1"
                     /translation="MYRIYLCRLYFFSLVLLTITSLLNASQSCIPINKIPVVINKSGY
                     YCLEKNHYYSGNQNPITINTSYVTLDFKSYKIELIPHNPANQIYGVYSLNSHHIIVKN
                     GQIKGFMMAVYLADSRGSKTSEGSHSGHYLIEKMTLQNNFFRGIRLEGVKQKINNCRI
                     LDTGGSTVYENAFSMGIEMIGPYSIVENNFIQDTKASGEGENIGISFSNNNANSVVTG
                     NHIFNHYGFLEKRARFRGDSGHSFGIWVGGNEKYRSNVLVHDNWIKNMYYGIVFSSVT
                     RGKVKDNKIESADKALLINSSVVVF"
     gene            complement(5739..6803)
                     /gene="rfbB"
                     /locus_tag="I9760_002369"
     CDS             complement(5739..6803)
                     /gene="rfbB"
                     /locus_tag="I9760_002369"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAT5044128.1"
                     /translation="MICQMLSTILQKSKNHTMKNQMNNLLVTGAAGFIGSNFVKFMND
                     KYPEIKIISLDKLTYAGNKANLSEMAECKNHLFVQGDILDKSLVLSLLREYEIDTLVH
                     FAAESHVDNSIDNPQIFLETNVIGTFTLLEAARIYWLNERQWDKSKCRFHHVSTDEVY
                     GSLEREEPAFTEKNSYQPNSPYSASKASSDHIVRAYYHTYGLPVTTSNCSNNYGPNQH
                     KEKLIPKVVYACVNQLPITVYGNGSNIRDWLYVMDHCEAIDTIIQKGALGEVYNIGGN
                     NELDNLSLIKMICQMMDDLKPMEKPYHSLITFVEDRKGHDKRYAIDNSKIQKELGWVP
                     QGDFVHKLSNTIEHYLKKYI"
     gene            complement(6763..7647)
                     /gene="rfbD"
                     /locus_tag="I9760_002370"
     CDS             complement(6763..7647)
                     /gene="rfbD"
                     /locus_tag="I9760_002370"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAT5044129.1"
                     /translation="MKILVTGANGQVGTEIIKRFSSSEHEVYACTRDILDCSKLERVH
                     DVLSEIKPDLIINAAAYTAVDKAEDEPDLAHIVNAEFVCHLVNYCSFKNIPLIHLSTD
                     YVFDGKKEGAYHETDIPHPVSTYGRTKWEGEQAILSQLKKYIILRVSWVFGEQGKNFV
                     KTILNLASSRKELNIVSDQWGRPTSARDIARVLFEIVQKIRHSSFDYWGLYHYAGQGV
                     TNWHEFANVFLNMAKEKQLLLTLTDLNPIKSEEYPTRATRPKNSVLDTTKIETILGIK
                     SCSWKNDLPDVIDHFTKK"
     gene            complement(7648..8211)
                     /gene="rfbC"
                     /locus_tag="I9760_002371"
     CDS             complement(7648..8211)
                     /gene="rfbC"
                     /locus_tag="I9760_002371"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAT5044130.1"
                     /translation="MNIVETRIPELKIIEPKIYGDERGFFYESFQAKRYEELLGITDR
                     FVQDNFSRSQKGVLRGLHYQSQQTQGKLVSVLAGEVFDVAVDIRLGSPTFGQWVGVIL
                     SGENKRQFWIPKGFAHGFYVLSAMADFAYKCTDYYHPESEFSIHYLDPQLAIDWPLGE
                     QVQLSPKDAAAKLLNLIDAELLPRYQA"
     gene            complement(8305..8472)
                     /locus_tag="I9760_002372"
     CDS             complement(8305..8472)
                     /locus_tag="I9760_002372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044131.1"
                     /translation="MNSKFEFSSILNKLITESGFFEIIKTAQLSDSEKWGVLDGKSGE
                     NLLNEIDLLFA"
BASE COUNT         2751 a         1704 c         1318 g         2720 t
ORIGIN      
        1 ccttacggtc gcggctcgga gtataatgac acgaaaccga gccgcgaccg taaggaagcg
       61 gatatagttg tgttaagacg cttgaaaatg catcgggtat attctttcaa caataaaata
      121 ataactaaat aaaatcagtg ttaacattaa taacgcgcat aatatggtca ctttctgtgt
      181 ccaactcaat aataccaaac atccaatgag tccattcatt agtaattgag caagtaatat
      241 taaacgggta tccattccaa attgttttaa tcgctggtaa gcatgtttcc tgtgcggagc
      301 aaaccaccgc tctttattca cgattcgtcg taataaagtg attgtagaat caaaaagaaa
      361 aaggctgttt aacataaacc aataaattac aggaatttgg tatttttgtt gggcaatcaa
      421 ggccattgag aaaataatta atcctaaaga agcactgcct atatccccca tgaataacct
      481 ggctggaggg aaattaaaaa ttaaaaatcc aactaaactg catattaaaa tccaacaaaa
      541 ttcctggtaa accaaagcat gatgtacatt aaataaaata aaatagcaag caagcaaata
      601 aatagcctgt gatgcgcaaa agccatccag gccatccatg aagttaaagt gatttatggc
      661 ccaaatcacc atgaaaaaaa caaaagggat gatgatataa tcatgactca tcgaaaaaag
      721 gataaaatcc agtttttctg gccttatgaa tacagctaaa atgaaagcga caatgcattg
      781 tactccgagc ctaatcttga tagggagatc gtatatatca tcaaaaaaac tgaccccggc
      841 tattaacaga atgctcaaca ataaaaacac cgattcttta taaaacgacc ctgtaaaata
      901 ccaaataaaa ggtaaaatta ataaagacaa accaataaat acaatgcccc cacctctaac
      961 cgttggtttc aaatgtagag ttctatcatt gggcttatca attagttttg aattttgtgc
     1021 aataccacaa aatagtttgg tacaaacaaa ggataacagt ataaaaaaaa cactaagcat
     1081 tatattcata ttgataccaa tctgctgttt tttttaagcc ctcacatgaa tcaactggtg
     1141 gagtccagcc taagtctgaa ataatctttg aattatcaat ttccagagaa ctcagcaagc
     1201 gagtatttaa actgcccatt cctgtgatct tgaacaggcc ggctaaaaaa gtagtaggga
     1261 agtgatacaa tcttaagttg attttcatat tttgggctag caaggataac aaatccgcta
     1321 aagaccaaga ttcattatct gctacaagat aaagttgatt tgccgcttta ggatgtgtca
     1381 ttaccattag aatagctgaa accaagttat caataaaaat aaaacttctc ttattcttta
     1441 tactggcaaa aggcaatggc catccttttt gtaccatacc caataatttt aaaaaattgg
     1501 cttttactcc aggtccataa acaagtggag gcctcaagat aaccacttcc atgtcactat
     1561 tttcactaat ctcacgcaaa tagagttccg cttctaactt actctgagca taagaatctt
     1621 ttggttgggc tgaatttttc tcagaaaatg ggctctgagg taagcttact tctccattta
     1681 ccttaatagt acttaaaaat acaaatcgct ttactttgtg ttttgcagct tgttgagcaa
     1741 aattcttggt ggcgatgctg tttattttgc aatactcttc cagagaggag gaggtatcct
     1801 ctctcataac atggactcgc gcagccagat gtatgacaat atcgattcca ttcaaagctt
     1861 ctgaccaatc cgttacttgc tccagcttat caatttcaac ttgctcaaca gacatgtatt
     1921 gatttttttg ccacacagca caacgaacct cataccctgc agcaataaga gccgggacca
     1981 agctactgcc aataaaacct gttgcgcctg tcactaatac ttttgtcact atattaccta
     2041 tggcttttaa aaccgtttat gattaagcag ttctgcaaga tattgaccgt aactactttt
     2101 attaagctct ctagccctgc aaagtaactc atcatcacta ataaacttca tacgccacgc
     2161 gatttcttca gggcatccaa tttttaaacc ttgccgctgt tcaatagtac agatatagtt
     2221 acttgctgat aacagggtct ctggtgtacc catatccagc cagacaaaac ctcttcccaa
     2281 aatctggaca tccagtttgt tttcatataa ataaaactga ttgatgtcag ttatttccag
     2341 ctcccccctc ccagagggtt ttaaactctt ggcaatttct ataacctgat tatcataaaa
     2401 atataatccg gttactgcgt agttggattt gggctgtgct ggtttttcca caatggatag
     2461 gacttgttgt ttttcattga actccaccac accataggcc tgaggtttat cgacataata
     2521 ggcaaaaact gttgcgccgt ttaattttga ttttgcattt tgtaataatt cagaaaaacc
     2581 tctgccctgg tggatattat cccccaaaac caggcaaaca gaatcattgc caataaattt
     2641 ttcacctaaa atgaatgctt ccgcgattcc ccttggttca ttttgcacaa gataggcaaa
     2701 tgagatgccc cactgagaac catcactcaa taaattttga tacagcggaa gatgctcctc
     2761 cgttgaaata atcagtattt ccctgatatt agctaacatt aatgtagaga tggcataata
     2821 aatcattggt ttatcataaa ccggaatcat atgcttacta atggattttg tcagaggata
     2881 cagtcttgta ccaaaaccac ctgccaggat aatacctttc attatatcga gcctactctc
     2941 tatttttctt tgaacaaata acagccttaa atattatcaa gtttattgaa gtaatacatt
     3001 aaaatacgtt gataatgtca ataaaatata aagtttagtt taataattaa actaatttat
     3061 agcattgagc gcccctttat gattcgcttg ataatgtgat ttctctgtga gcactcttgg
     3121 cgctttcaat accgggctga tcgaatgggt taatattcca tatcacactt tgtacaaaaa
     3181 tcttatgttc atagagtgcc accagagccc ctaatgtata aggagaacaa tctgacaaaa
     3241 tgagatgatt cattggcata ttgccaggaa tatacccata aggattttca attgtttgaa
     3301 ttccctcact taaaactttc attttataat gacacatgga attaatctca tgctcatcat
     3361 tagttttcaa agtaataaaa tcgccaacac atcgatgcgt accctgacat aataattgaa
     3421 aatagctatg ctgtgcctga ttacctaatc caccccaaac gataggtcca gtggcgtaat
     3481 ctaccattct tccactcaca tcaatggact tcccattact ctccatatcc agctgttgta
     3541 cataaggaac aaagtactcc agtttttttg aataggtaag aataagtaaa ttatgaatat
     3601 tcaaaaaatt gttattccat ataccgatta aagccattag cacaggaata ttatttttaa
     3661 aatcagtaaa ttggacatga gtatcgatat catgggcacc agccagcaat tcaacaaatt
     3721 gttcataacc aattgcaatc gcagtaatta aattaactgc ggaacaaaaa gaaaatcgac
     3781 caccaaccca atcccaaatc ggtaaaacag ttttaatacc catttgataa gctctttccg
     3841 gatgtgcggt tacagctata aaatgttgat cgatagaggc cgtatcctca tacaatgcaa
     3901 aagcttttct ggcatttaat agagtttctt ttgtagtaaa tgatttggaa gaaacaataa
     3961 ataaggtggt ttgcggatta atttttgcaa taacatcgtt aaaactggcc ggatcaacat
     4021 ctgagataaa atgataatta aattctttgg aaatataatt agacagggca ttgatgcata
     4081 cacgagaacc taaatccgag ccgccaatac ctatgtttac aatatcagtt attggtagac
     4141 ctgaatggcc cagccacttc ttttctctga ttcggttaga tatcactctg attttttctc
     4201 gggtacttac cacttcagac atgatatcca gcccatcaat caggattgat ttattgctta
     4261 aatctcgcag tgccgtatga agagctggcc tattctcact gatatttact ttctctcccc
     4321 tgattaagcc gtcaattttt tcttgtaatt ttatctcatt tgccaaattg ataagcaaat
     4381 cgagaatagt gttatttact ttttgtctgg aataatcata ctcaatggta ttaatttgtt
     4441 tgcaataatc caaatgcatg ccattcatga atggcgtcaa ttgcgatcct gaagttaatc
     4501 gaattttatc tgcttcttgt cgaagtaaat tccacattgg taattctgtc tgtcgcttca
     4561 tgctatttct aatgattaaa atgagtaata aactaccata attaagaaag ttgtaaacct
     4621 tatagctcta taaaataccc aaaaattggg attattatac tcttaacagg tttgttgaca
     4681 attcaagcca gaatctacac cgcacgctgt aggagcagcg tccatggatc tcgtagataa
     4741 acaacggcta gtcggaattt tatattgaat tgaaaacaag ccctaatatc ttattcttaa
     4801 aataccacta cagagctgtt aattaataac gctttatccg cactctctat tttattgtct
     4861 tttaccttcc ctcttgtaac ggatgaaaaa acgatgccat agtacatgtt ttttatccaa
     4921 ttatcatgaa ctaaaacatt ggatctgtat ttttcatttc caccaaccca aatcccaaaa
     4981 ctatgcccag aatcccctct gaaacgagct cttttttcca aaaaaccata atgattgaaa
     5041 atatgattgc ctgttacaac agagttagca ttgttatttg aaaatgaaat gccaatattt
     5101 tctccttctc cggaggcttt agtatcctga ataaaattat tttctactat tgaatacggg
     5161 ccaatcattt caatacccat gctaaatgca ttttcataga ctgtgctacc acccgtatca
     5221 agtattcggc aattattaat tttctgctta accccttcta atctgattcc tcggaagaaa
     5281 ttattctgca atgtcatctt ttcaataaga taatggcccg aatgacttcc ttcactcgtc
     5341 tttgaacctc tgctatcagc aaggtatacc gccatcataa atcccttaat ctgcccattt
     5401 ttcacaataa tatgatgaga gttcaaggag taaactccat atatctggtt agcagggtta
     5461 tgtggaatta actcaatttt gtaacttttg aaatccaatg ttacgtaaga agtatttatt
     5521 gtgattggat tttgattacc tgaataataa tgattttttt ccaaacaata atagcctgat
     5581 ttgttaatga caacagggat tttattgata ggaatgcaac tctggcttgc atttaataga
     5641 gaggttattg ttagcagcac taaactaaaa aaatacaacc tacataagta gattctatac
     5701 atgcctctcc ttgctaaaat caattgtatt gataggttct atatatattt ctttaaataa
     5761 tgctcaatgg tgtttgataa tttatgaaca aaatcacctt gtggaaccca acccaattcc
     5821 ttttgaattt tactattgtc tatggcgtat cgtttgtcat gaccttttct atcttcaaca
     5881 aaagtaatca atgaatgata tggtttttcc ataggtttta aatcatccat catctgacaa
     5941 atcattttta tcaaacttaa attatccagt tcgttattac cacctatatt ataaacttcc
     6001 cctaatgctc ctttttgaat aatggtatca attgcttcgc aatgatccat gacatacaac
     6061 caatcgcgaa tatttgatcc gttgccataa acggtgatgg gcagctgatt gacacaagcg
     6121 tatactacct taggaatcaa cttctcttta tgttgattag gcccgtaatt attcgaacaa
     6181 ttcgaagtgg taacaggtaa tccataggta tgataataag ctctgactat atgatcagaa
     6241 cttgctttgg acgctgaata aggagagtta ggttgatatg aatttttttc agtgaacgcc
     6301 ggctcttctc tttctaatga accatacact tcatcggtgg atacatgatg aaaccgacat
     6361 tttgatttat cccattgcct ttcattcaac caataaatcc ttgccgcctc taacagagta
     6421 aaagttccaa ttacattggt ttcaagaaat atttgcggat tatcaatgga attatccaca
     6481 tgggattcgg ctgcaaaatg gacaagcgta tctatttcat attcccttag taaggataac
     6541 accagacttt tgtccaatat gtctccttgc acaaaaaggt gatttttaca ttctgccatt
     6601 tcactcaaat tggctttatt tcctgcatag gttaatttat ccaaactgat tattttgatc
     6661 tccgggtatt tatcgttcat aaatttaaca aaattacttc ctataaagcc cgcagctcca
     6721 gttaccaaaa gattgttcat ctgatttttc atagtgtgat tcttactttt ttgtaaaatg
     6781 gtcgataaca tctggcaaat catttttcca actacagctt tttataccca atattgtttc
     6841 tatctttgtt gtatcgagta ccgaattttt aggccttgtt gccctagtag gatattcttc
     6901 tgacttaatt ggatttaaat cggttaaagt caagagaagc tgtttctctt ttgccatatt
     6961 aagaaatacg tttgcaaatt catgccaatt ggtaacaccc tgccctgcat aatggtaaag
     7021 cccccaatag tcaaaggaag aatgtctgat tttctgtact atttcaaata gtactcttgc
     7081 tatatcgcga gctgaagtag gccttcccca ttgatctgaa acaatattca attcttttct
     7141 gctggaagcc aaatttaaaa tcgtttttac aaaatttttc ccttgttcac caaataccca
     7201 gctaactcgc aaaataatat actttttaag ttgggataaa atagcctgtt ctccttccca
     7261 tttcgtacgc ccataggtac ttactggatg cggaatatcg gtttcatgat aggcgccctc
     7321 tttttttcca tcaaatacat aatcagttga aagatggatc aaggggatgt ttttgaagct
     7381 acaataattc accaggtgac agacaaactc ggcgttcaca atatgagcca aatcaggttc
     7441 atcctctgcc ttatctacag ccgtataggc tgctgcatta atgataagat ccggcttgat
     7501 ttccgataac acatcatgaa cccgttcaag tttggaacaa tctagaatat ctctggtaca
     7561 agcatatact tcatgctcag aggaagaaaa tcgcttgata atctcggtac caacttgccc
     7621 attagcgcct gtaaccaata ttttcattta ggcttgatat ctcggtaaaa gttcagcatc
     7681 tattaaattt aaaagcttgg ctgcagcatc ttttggagac aattgaacct gctctcctaa
     7741 tggccaatca attgctaatt gcggatcaag ataatgaatg gaaaactcac tttcaggatg
     7801 ataatagtcg gtacatttat aagcgaaatc agccatagca ctcaaaacat aaaacccatg
     7861 agcaaaccct ttaggaatcc aaaattgcct tttgttttct ccggaaagta tcactccaac
     7921 ccattgccca aaagtagggg agcctaacct aatatccacg gcaacatcaa acacttcacc
     7981 agctaaaact gaaaccagtt ttccctgagt ttgttgactt tgataatgca aacctcttag
     8041 tacccctttt tgtgatcttg aaaaattatc ttgtacaaaa cgatcagtta ttcctaataa
     8101 ctcttcatat cgtttagctt gaaaagattc ataaaaaaac cccctttcat caccataaat
     8161 ctttggttcg ataattttaa gttctggaat tctcgtctca acaatattca tcataaaaat
     8221 ctatcttcta acactgctaa aaattggggg cttatttaaa aataaatgat taaatttata
     8281 tctctaaatt atatctataa aaaattaggc aaacagcaag tctatttcat ttaataaatt
     8341 ttctcctgat tttccatcta aaacacccca tttctcacta tcagataatt gcgctgtttt
     8401 gattatttca aaaaaaccgg attcagttat tagcttatta agtattgagg aaaactcaaa
     8461 cttactattc atggtcagaa cgccttttat att
//
LOCUS       DACTJE010000143         8413 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.143, whole genome shotgun sequence.
ACCESSION   DACTJE010000143 DACTJE010000000
VERSION     DACTJE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8413
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.143"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..390)
                     /locus_tag="I9760_002373"
     CDS             complement(<1..390)
                     /locus_tag="I9760_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAT5044132.1"
                     /translation="MIVNSSFRPLWWLRNKHAQTLYPTLVKKRLKPPIDFHERLELPD
                     GDFIDLAWSVNDLNKNTPLVILLHGLGGGINSIYVSGLMQAFANAGFRCVLMHFRGAS
                     EEPNRILRTYHSGDTADFAYFLEILANR"
     gene            661..1677
                     /locus_tag="I9760_002374"
     CDS             661..1677
                     /locus_tag="I9760_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAT5044133.1"
                     /translation="MYSLDWPRAYGIPNSTATFKLFPEDFQVNELFEGQFSGEGEHIV
                     LKIEKRGLTTEEVVKSLARLINKPIKLISYAGLKDKQALTTQWLSVHAPGEIIDGIET
                     LEAPGWRILECTRHNKKLKPGFLSGNHFIITLRNVSDESDLVHRIEQIKFKGVPNYFG
                     EQRFGRDGGNLIKAEEILVQGRKVKDRFLKGMYFSAARSWLYNLILSRRVKESSWNLP
                     LLGDVIQLVGSNSIFVNDKSMDEQLLQRIGDKDVSPASPLPGRSKNLVKGTALQIIND
                     VYEEWSAWLDGLEKNGLEEAWRANILYAEQIEYRINQGTVELSFVLPAGAYATVVLRE
                     LVQY"
     gene            1810..2568
                     /locus_tag="I9760_002375"
     CDS             1810..2568
                     /locus_tag="I9760_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044134.1"
                     /translation="MNLVQRMNPFFWLVILAIYSTPSMALSVELIETSPKSPVTLNQD
                     EALYVLIRYKSDQPLRFQAIGENLRQKIMDSARFNPSQAYPAGEGEAIAWVAYDNTTE
                     IDSITVTIYDANWRTLQTKSIPVSAVWQNENGRNNQAAAPWVQRLNQQQQSSVFTQSQ
                     TPVSSGNALFIQLLFLLILLYWFLQIIVIFNWTGRWKKLACFPLLFSVPLLLYTLYAL
                     YAGSNLWPLMMLFVTPFILALLLIIIGYKKVYSR"
     gene            2688..4007
                     /gene="ceg23"
                     /locus_tag="I9760_002376"
     CDS             2688..4007
                     /gene="ceg23"
                     /locus_tag="I9760_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg23"
                     /protein_id="HAT5044135.1"
                     /translation="MHTKKDKKVISLQERVENAVDVSGAFDNCFFHNFALYLLTNNLP
                     LPDDLFHFKSIINRNSKAEQLFEFFHNPESLNLFSILDKENDVSEPSGYLFEKSLILG
                     FLLREWFPTQLVNNSAVKAEMLEGEKGVFSAFKNYKEYRSFMSKEELKSTEFGALYEA
                     NEAFLEYFYNRSESTLINKDSPFEKYFVGSSSDEEAIKNYWDAEGYTLYCQHLAKPQV
                     KLSYIEIMTMMKVINQPLTIYDRSTSSIVAEYVNPKVNLPDFEVAIDALQGHYFLLKT
                     EETEKELEEYERSYAQYKRDRSEILAHSDKPVSSLLVRATCPKGHLDEDPFIALIESL
                     SEINSLSQIDTNLKNENTDIPNCNFLLKVGASVVGTSSALITLMGIAILSGQYDPGNS
                     LAEEALATTVVSSIVGLGYGLYSFFSSSPKVSERDSTNGLSQKLKIN"
     gene            4198..4617
                     /locus_tag="I9760_002377"
     CDS             4198..4617
                     /locus_tag="I9760_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT5044136.1"
                     /translation="MSAKKKSITHDRLLTEVELELMNIIWSLDKVTIKEVVSHLPKER
                     PLAYTTVATVLKVLEQKGFLECQKNSYAHVFTPIVTKSDYESTCIDHMVANVFDGEPR
                     ALVQRLLLARKLQHDDIQAIEEALKQLNAGEKQPEVM"
     gene            4617..6527
                     /locus_tag="I9760_002378"
     CDS             4617..6527
                     /locus_tag="I9760_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HAT5044137.1"
                     /translation="MLLIEIVLTIHCLLLLSSLFIGRAWLANKPSFKLKLLRFLLASC
                     IISPIIVHCVDSDQKPERQNYVSFDALQQYAKQSIFKTESSKFNHGSSSAFTSMNVNY
                     CQLLSVMFFLIVLFRSYHLLSGLGRLKLMLTEAIPYRTSGKLVIKVSHHCHVPFSILL
                     FNKAYIVLPVSIFSSAMNVRIAIAHEGQHHRNGDCSWAYFIEIIRIIFFGNPGVTQWF
                     RVLADLQELSCDEVLVGHQKISAHDYGHCLLQVAEAVSQCSLASNREIACTVGMALNK
                     ENEDCKFIIRRISMLTAYSPNASRPLLLGIVFAGFSILSPICVAYSTAGSLTSSRAKE
                     VDISHLDPRMQQIVEKQITTAVKQYHAKSGVIAIADPQTGNIIAFAESSKNKGLESWK
                     SRIFSPGSTIKPFIAAAAINSGNSSETKNYDCHSPYYIAGKTFTNYNSNVESASLADA
                     IAKSINVCLIRVSQEAGVPVIRKKLTEFGFDMNSWWQADQSDDLQLAMAALGENIPVT
                     IESLIKSYAILANKGHSFDRGNSAIISETSTNSINHMLENAVTNGTGKLAVIPGVSVA
                     GKTGTVIENNDKYLALFAGYVPADNPRYVLLVVIEEGYFSKNGKTLVSGGELAAPVFR
                     NVAMDALSSANR"
     gene            6577..7377
                     /gene="blaOXA"
                     /locus_tag="I9760_002379"
     CDS             6577..7377
                     /gene="blaOXA"
                     /locus_tag="I9760_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HAT5044138.1"
                     /translation="MKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQR
                     YSPASTFKIALSLMGFDSGILKDELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVW
                     YSQALTRQLGMKRFKGYVDAFHYGNQDVSGDRGQNNGLTHAWLSSSLSISPTEQIQFL
                     QKIIYKKLPVSQKAYIMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWF
                     VGWIEKDERVITFAKHIADSKENNTFASFRAKNDALIQLFNLINELEK"
     gene            complement(7502..8374)
                     /locus_tag="I9760_002380"
     CDS             complement(7502..8374)
                     /locus_tag="I9760_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HAT5044139.1"
                     /translation="MDKNLKLMFLLVYVISLVLLSACCFGAPNSIEKQLKIIIANKAY
                     TLSDLKKQFKTIRVSIPFNPAYNNVKKVYYAFDMNQILTKLLAVDLQKNNVDQVLLAQ
                     TADNYMSQTPLLYFTTKGQAYLAYQEAPETISDKDITKDGRWSYINQHGKKDNPGPFY
                     IVWDNTSTYPAGWPYQVISIQIVNKKDLAFSRFLNPLHESESIKNGHHIFNNMCSTCH
                     SIFYKGAQGRAPDLGKVTSYLTPSDISKLVKHGRGYMPPIGKNLSTEEINDLIKFLIW
                     VNRQSSKLKCEIND"
BASE COUNT         2667 a         1454 c         1639 g         2653 t
ORIGIN      
        1 tctatttgcc aaaatttcaa gaaaataggc aaaatcagcc gtgtcgccgg aatgataggt
       61 acgcaaaatc cgattaggtt cttcactggc tccgcgaaaa tgcattaaaa cacaacgaaa
      121 cccagcattg gcaaacgctt gcattaatcc actgacataa atagagttaa tacctccacc
      181 caaaccatgg agtaatatga ctaagggtgt atttttattt aagtcattaa cagaccaggc
      241 cagatcaata aaatcaccat caggtaactc cagacgctca tgaaaatcaa ttggtggttt
      301 tagccgcttt tttaccaaag taggatataa agtttgcgca tgcttgtttc ttagccacca
      361 caggggtcta aatgaactgt ttacaatcat attaatagcc tgaaaataaa aaatcgaact
      421 ctatccggat aaattagacc aagattagaa gtagcaaaaa aatacttact taaattatag
      481 tcaatcaata attgaaacca atcattgttg gtaatcttgc atcataatgt tttatagtcg
      541 ctatatcaaa cgatgttaag ataaataatg tatcagctaa accaataagt tctgaatctc
      601 ttgatatcat agtgaatttg tagatttagt ttagaaccct ttttattgaa ggagatcttg
      661 atgtactctt tagattggcc acgtgcctat ggtattccaa attctacagc tacttttaag
      721 ttgtttccgg aagattttca agtgaatgaa ttgtttgaag gacagtttag cggtgaagga
      781 gaacacatag ttcttaaaat tgaaaaaaga ggattaacca cggaagaagt tgtcaaatca
      841 ttggccaggc taattaacaa acctataaaa cttatcagtt acgctggatt aaaagacaaa
      901 caggcattaa caacgcaatg gcttagtgtt catgcacccg gggagataat cgatggcata
      961 gaaacacttg aagcaccagg ttggagaata ctggagtgca cacgtcataa caaaaaactc
     1021 aaaccaggtt ttttatcggg caatcatttt ataatcactt taagaaatgt ctctgatgag
     1081 agtgatctag tgcatcgtat tgaacaaatt aaattcaaag gtgttccaaa ttatttcggt
     1141 gagcagcgtt ttggtcgaga tggtggaaat ttaattaaag cagaggaaat actagtacag
     1201 gggcgcaaag taaaggatag atttttaaaa ggaatgtatt tttccgcagc ccgatcatgg
     1261 ctatataatt tgatcctgtc tcgccgtgta aaggaatcat catggaattt gcccttattg
     1321 ggggatgtca tacagcttgt tggatcaaac agtatatttg ttaatgataa aagcatggat
     1381 gagcaattat tgcaacgaat cggtgacaaa gatgtttctc cagcaagccc tctgcctggc
     1441 aggagtaaga atcttgtaaa agggacagct ttgcaaataa taaacgacgt ttatgaagag
     1501 tggtctgcct ggcttgatgg gttagaaaaa aatggtttgg aagaagcctg gcgtgcaaat
     1561 atcctttatg cagagcagat tgaatatagg ataaaccaag gtacagttga gctttcgttt
     1621 gtcttgccgg ctggtgctta cgcgacagtg gtattacgtg aattggttca atattaagct
     1681 ttgtttgcac ttgaatagca ctttagtcaa tgtggagtaa ttatttttaa ttgaattgat
     1741 gaattaatca ctatgtttga tgttatgcca taataaagag aaatgtttat ttaattgaag
     1801 gacacattca tgaatctggt tcaaaggatg aacccttttt tttggctagt gatcctggct
     1861 atttattcaa ctccatccat ggcattgagt gtggagctta tcgaaaccag tcctaaatct
     1921 cctgttacat taaaccagga tgaggctttg tatgtgctta ttcgatataa gagtgaccaa
     1981 cctttgcgat tccaggctat tggggaaaat ctgcggcaaa aaataatgga tagcgccaga
     2041 tttaacccat cacaagcgta cccagccgga gaaggtgagg ctattgcctg ggtagcttac
     2101 gataatacca cagaaattga ttccattact gtcacaatct atgatgccaa ttggaggact
     2161 ttgcaaacca aatcaattcc tgtgtctgca gtctggcaaa atgagaatgg cagaaacaat
     2221 caagcagcag ccccctgggt tcaaagactg aatcaacaac agcaatccag tgtttttact
     2281 cagtcacaaa ctcctgtctc ctctggtaat gccctgttta ttcaactcct ttttttacta
     2341 attcttttgt attggttttt gcaaattatt gttattttta attggacggg gagatggaaa
     2401 aaattggcgt gtttcccttt attatttagc gtacctttgc tgttgtatac gctttatgcg
     2461 ttatatgcgg gttctaattt atggcctttg atgatgttat ttgtgacacc atttatcctg
     2521 gcacttttat tgatcattat cgggtataaa aaagtctatt ccagatgata agatgtatcc
     2581 aatttaatct attattatat tttatctctt aatatttgct taataatgat tctttataat
     2641 caaaaatcaa tgtaattgtt tcaaataaca aagagataga ataaattatg cacacaaaaa
     2701 aggataaaaa agtaatatca cttcaagaaa gagtagaaaa tgcagttgat gtttcggggg
     2761 cttttgataa ctgctttttc cataatttcg cactttacct gttaacgaat aatttaccac
     2821 ttcctgatga tttatttcac ttcaaatcaa tcattaatag aaatagtaag gcagaacaat
     2881 tatttgaatt ttttcataac ccggaatcat tgaatttatt ttctatatta gataaggaaa
     2941 atgatgtttc tgaaccctct ggttatctct ttgaaaagag cttgatttta ggatttctgc
     3001 ttagggaatg gttcccaact caacttgtta ataattcagc agttaaagcg gaaatgctgg
     3061 aaggtgagaa aggggtattc agtgctttta aaaattataa agagtatcgc tcatttatgt
     3121 cgaaggaaga attaaaatca acggaatttg gtgcgttata tgaagcgaac gaggcttttt
     3181 tagagtattt ttataatcgc tctgaaagta ctttaataaa taaggattca ccttttgaaa
     3241 aatattttgt tggctcgtcc tcagatgaag aggcaattaa aaattattgg gacgcagaag
     3301 ggtatacatt atattgtcaa catttggcga aacctcaggt taaattatct tatattgaaa
     3361 ttatgaccat gatgaaggtc atcaatcagc cacttacaat atatgatcgt tctacttcct
     3421 caattgtagc cgagtatgtc aacccaaagg tcaatttacc tgattttgaa gtggctattg
     3481 atgctcttca agggcattat tttcttttaa aaacagagga aacggaaaaa gagttagaag
     3541 aatatgaaag aagttatgct caatataaaa gagatagaag tgaaatcctt gcacactccg
     3601 ataaacctgt ttcttcctta ttggttcgag ctacttgccc caaaggtcat ttggatgaag
     3661 atccttttat tgcattaatt gaaagccttt ccgaaataaa cagccttagc caaattgata
     3721 ctaatttgaa aaacgagaat acagatattc ccaattgcaa cttcctttta aaggtaggtg
     3781 cttctgtagt tggtacctct tctgcgctaa taactttaat ggggatagcc atattatctg
     3841 gccaatatga tcccggaaac agtctggctg aagaggcatt agctactact gttgtatcca
     3901 gtattgtggg tttaggttat gggctttata gcttctttag ttctagcccc aaggtatcag
     3961 agcgtgattc taccaatgga ttatcgcaaa aacttaaaat aaactaacct cctgaattag
     4021 caaatattgt ctgtaagccg atcgtgttga tattagatat caacacgatt gcgccttgag
     4081 taaactgttc tcacgtgtag agaagtattt tccccatgac tattttaact ctactatttt
     4141 tatagtagga tactactata aaaatagtag tagcgtatgt ggttattgga gagaaacatg
     4201 tcggcaaaaa aaaaatcgat aactcatgat cgacttctta cagaagtaga gcttgaatta
     4261 atgaatatta tctggagcct tgataaggta actattaagg aggttgtttc acatctgcct
     4321 aaggaaaggc ctttggctta tacaaccgtg gcaacagtac taaaagtgct ggaacaaaag
     4381 ggatttcttg agtgtcaaaa aaattcctat gctcatgttt ttacacctat agttacaaag
     4441 tcagattatg aaagcacttg tattgatcat atggttgcca atgtttttga tggggagccc
     4501 cgagcacttg tccaaagact tttgttagcc agaaagttac agcatgatga cattcaagcc
     4561 attgaagaag cacttaaaca attaaacgct ggtgaaaaac agccagaggt gatgtaatgt
     4621 tattaataga aatcgtattg actattcatt gcctgcttct tctcagttca ctatttatag
     4681 ggcgtgcctg gctagcaaat aaaccatcat ttaaattgaa attactccgt tttttattgg
     4741 ccagttgcat tatttcacca ataatagttc attgtgtaga ctctgatcaa aagcctgaac
     4801 gacaaaacta tgtatcattt gatgctttgc aacagtatgc aaagcaatcc atatttaaga
     4861 cagaatcttc aaaatttaat catggatcgt cttctgcctt tacatcaatg aatgtgaact
     4921 actgtcaact cttaagcgtg atgttttttt taatcgtctt gtttcgtagt tatcacttgt
     4981 tatcaggttt aggtcgcttg aaattgatgt tgactgaagc aatcccatat cgaacttccg
     5041 gaaaattagt tattaaggtt tctcaccact gccatgttcc tttctcaata ttgctattta
     5101 ataaagccta catcgtattg ccagtatcca tattttcttc tgccatgaat gtgaggattg
     5161 ctattgctca tgaagggcaa caccatcgta atggagattg ttcatgggcc tattttatcg
     5221 aaatcatacg cattatcttc tttggaaacc cgggtgtaac tcaatggttt cgtgttttag
     5281 cagatttaca ggaattgtca tgtgatgaag tattggttgg acatcaaaaa atttcggcac
     5341 atgactatgg tcactgtcta ttgcaagtgg cagaggcagt atcccaatgc tctctggcat
     5401 ctaaccggga aattgcatgc acggtgggta tggctttgaa caaagaaaac gaagactgca
     5461 aatttataat aaggagaatt tcaatgctaa ctgcttattc acccaatgca tccagacctt
     5521 tgctattagg gatagtcttt gcaggatttt ctatcctgtc tccaatctgc gttgcctatt
     5581 caacagcagg atctttgacg agttccaggg caaaagaagt tgatatttct catttagatc
     5641 ccagaatgca gcagattgtg gagaaacaaa ttacgacagc cgtcaaacaa tatcatgcaa
     5701 aatctggagt aattgcaata gctgatcccc aaacaggcaa tatcattgca tttgctgaat
     5761 caagtaaaaa taaggggcta gagagctgga aatcacgtat tttttcaccc ggatctacta
     5821 taaaaccttt tattgcagca gctgcaatca actctgggaa tagctctgaa acaaaaaatt
     5881 atgattgtca ttcaccttat tacatagcag gcaaaacatt tacaaattat aattccaatg
     5941 ttgaatcagc ttccttagca gatgccatcg caaaatctat caatgtatgc cttatcagag
     6001 tttctcaaga ggctggagtg ccggtaatcc gtaaaaagct cactgaattt ggttttgaca
     6061 tgaacagttg gtggcaagcg gatcaaagtg acgatttaca actggcaatg gctgcacttg
     6121 gagagaatat ccctgtcaca atcgaatcgc taataaaatc ttatgccatt cttgctaaca
     6181 aaggacattc atttgacagg ggaaatagtg ccattatttc agaaacaagc actaactcaa
     6241 taaatcatat gcttgaaaat gctgttacaa atggcactgg aaaactcgca gttattcctg
     6301 gtgtttctgt agctggtaag acaggaactg taattgaaaa taatgataaa tatcttgctt
     6361 tgtttgccgg gtatgtgcca gctgataatc cgcgttatgt tttattagtc gtgattgaag
     6421 agggttattt cagtaaaaat ggaaagacat tagtttcggg aggagaattg gccgcaccgg
     6481 tattccgaaa tgtcgcaatg gatgctctga gcagtgcaaa tcgataacta gcttatgggt
     6541 ttgcatatcc atatattcaa aaatgttgag gttgttatga agaaaataat tatatttttg
     6601 tgtacaggat tattattttg cgtctctact tgggctcaag gcacctgttt tttagcaaaa
     6661 gaaaatcaaa ctgttttaaa acgtgagggc aatgattgcg atcaacgcta ttcgccagca
     6721 tcaactttta aaattgccct tagcctcatg ggctttgatt ctggaatatt aaaggatgag
     6781 ttgcatcctg aatggccata caaaaaagaa tatgaacttt atctgaatgt atggaagtac
     6841 cctcaaaacc cgcatacctg gatcagagat tcctgtgttt ggtattcaca ggcattaact
     6901 cgacaattgg gcatgaaacg gtttaaaggg tatgtcgacg catttcacta cggtaatcag
     6961 gatgtttcgg gagacagagg tcaaaataac ggcttaaccc atgcctggct ttccagctca
     7021 ctttctatct cacccacaga gcaaattcaa tttctacaaa aaataatcta caagaaacta
     7081 ccggtgagtc aaaaagctta tatcatgact aaaaatatca tgtacatcca ggagttgcct
     7141 ggtgggtgga agctttatgg aaaaacagga actggtcggc agttaacaaa agataaaagc
     7201 caaaaattgc ctttgcaaca tggatggttc gtaggttgga ttgagaagga tgagcgtgta
     7261 attacctttg caaagcatat tgcagatagt aaagagaata acacttttgc cagttttaga
     7321 gcgaaaaatg atgctctgat tcaattgttt aacctcatta atgagttgga gaaataatct
     7381 aagacattgt cggagcagga ttattctgtt ccgattaccc aaatctgcca actatagtca
     7441 cacacattcc aggagttgct gctgcttgtc atcggtagct acaaagtcct aaattatatc
     7501 cttagtcatt aatttcgcat tttagtttgg atgattgtct gttaacccag attaaaaatt
     7561 taatgagatc attaatttct tccgtagaga ggtttttgcc tatcggtggc atgtatcctc
     7621 gtccatgctt tacaagctta ctgatatcag aaggtgttaa gtagctcgtc actttgccta
     7681 agtctggtgc gcgtccttga gcacccttgt aaaatataga gtggcaagtg gagcacatat
     7741 tattaaaaat atgatgacca ttttttatag actctgattc atgcaaagga tttaagaaac
     7801 gagaaaaagc aaggtctttt ttattcacta tttgaatcga aataacctgg tatggccaac
     7861 cggcagggta ggtgcttgta ttatcccata caatatagaa agggccggga ttatcttttt
     7921 taccgtgttg attaatataa ctccagcgac catctttggt gatgtccttg tcagaaattg
     7981 tttcgggcgc ttcttgataa gcaagatagg cttgaccttt tgtcgtaaaa tacaacaggg
     8041 gggtttgaga catatagtta tctgctgttt gagctaataa tacctggtct acattgttct
     8101 tttgtaaatc tactgccaac aattttgtta aaatctgatt catatcaaaa gcataatata
     8161 cttttttcac attattataa gcaggattaa aggggatgct aacacgtatg gttttaaact
     8221 gttttttaag atctgacagc gtatatgcct tatttgcaat aattatttta agttgttttt
     8281 ctattgaatt gggtgcccca aaacaacatg ctgaaagtaa tacaaggcta attacataaa
     8341 ccagtagaaa cattaatttt aggttcttat ccataagtcc ttcttgtttt ttgaaaacaa
     8401 ttcagatttt ttc
//
LOCUS       DACTJE010000144         8365 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.144, whole genome shotgun sequence.
ACCESSION   DACTJE010000144 DACTJE010000000
VERSION     DACTJE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.144"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..488)
                     /locus_tag="I9760_002381"
     CDS             complement(<1..488)
                     /locus_tag="I9760_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAT5044140.1"
                     /translation="MSTLQFDNFIIQLAGEIDKLTNHFVFDGYTALASLLKAPLGAMI
                     VLYIILKGYGIALGVIERPQQELFRFSMRAGLIYMMAMNWDLFSSYMRDLFVSGSESI
                     ATQLMQAVQKNPSSGSINQGLQNVLNEILKLGSDLFEAGSLRKLTPYFAGMMVFLSGS
                     VT"
     gene            complement(492..887)
                     /locus_tag="I9760_002382"
     CDS             complement(492..887)
                     /locus_tag="I9760_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein LvrD"
                     /protein_id="HAT5044141.1"
                     /translation="MNTLIKGLMLVLMAATLSACLHENPLKTQPAKISVTFLINASAN
                     AEKRLHFAIHKDAYGFAYLECMEGKTSHEIRCDELYQGMIAFAKEGHYSGFQKITLDD
                     LTDHGLFETLADTYAETAATIWPHFVSRT"
     gene            complement(1045..1755)
                     /locus_tag="I9760_002383"
     CDS             complement(1045..1755)
                     /locus_tag="I9760_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044142.1"
                     /translation="MKRAACFLIAAMGISSASHADILGVEDAQLIVLAMKQLGELKEQ
                     YRVLSDTYQTAKNQLDNLNQLKSMNSGHYGFGDFNNSLDSLQSWQSPVSTWQDALQNL
                     SGGNQQRYQALMDAYEKNHPALDENSYARFTTPANAARFKEDKAVNRAVMVQTTESYN
                     EINKHMEALHKLSQQIEKTPNTKGAIDLNSRLITEIGFIQLMSLRLQALISQQAAQEN
                     LSALQDRAEMAKFNRLPK"
     gene            complement(1752..4232)
                     /locus_tag="I9760_002384"
     CDS             complement(1752..4232)
                     /locus_tag="I9760_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HAT5044143.1"
                     /translation="MNLSKTLKHVYQEAKVSDLFPVTHLNSPSIFESQSGLVGSVIKV
                     NGAAFEIEEAETLNHQSFLLHQALVSLDSRFIIYVTTHRQKISCPLIGEFKPGFAKDL
                     DEHYQQRFKDKNLYKNTLYITIVLKGDDSSKTGSWLQWAKSLSMKGNSELSEHHREQN
                     IQILNRVVEQLQANLNPFGATILGKQDEALGFSELLQFLGLVVNAGQTISFKHPVYSP
                     PIANSIPQSFRQEAKYPEGHLGQYLSSCQLLFGECIQFQGNTQKDCVFGAMLSLKKYP
                     TNTASILLDSMLSLDCEFIATHTFAPIGRDSALNMISKKRSKLLSAEDKALSQTTALS
                     DLEDGIASETLLLGAHHHTVMLLAPNKPLLDTAILEATKRYGASGIVVVKETLGQEPA
                     FWSQLPCNQHFITRASLITSQNFVDFCPLHNTQTGFSNENFLGGAVTLLETPSKTPVY
                     FNYHARGSKTNPSKGHAAIFGGNNAGKTTLVNFLDAQMGRFGGRSFFIDRDESSKIYV
                     LASGNSSYIKIEPSNPIAMNPLQLPDTSENRSFLKLWLATLVQEESECVIPSQIAEII
                     NDCIDYSFEQLAPEFRTLSHLSQFLPVDFPRWPHLKRWLKQNDSRIDGEFHWLFDNPN
                     DALNLDFDKVGFDVTYMMDQVHSVIATPVYLYLLHRMRQCLDGRLTSFIIAEAWQLFA
                     SPFWEKALREWLPTIRKKNGHFIFDTQSPKTITDSPIKHIVLDNLATLIAFPNPLADK
                     ETYMEHLKLTEAQFEAIKENTPESRIFLYKQDHKAFLCKLDLSGLSQYIRVLSANTQS
                     VKLLDDIMQEVGHDPELWLPVFYERSKK"
     gene            complement(4219..4500)
                     /locus_tag="I9760_002385"
     CDS             complement(4219..4500)
                     /locus_tag="I9760_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044144.1"
                     /translation="MSDLEISPIFNALTRPAMTAGVTFEYHMLNLIVSMCAFIGFSPL
                     YGLIFIPLHVFGWLVCRYDTHFFTICAKRFLLPEAPNTSLWRVRAYEPF"
     gene            complement(4510..4800)
                     /locus_tag="I9760_002386"
     CDS             complement(4510..4800)
                     /locus_tag="I9760_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HAT5044145.1"
                     /translation="MSKLKRWSQSLGNLYLIAVCTLLPTLSHAQSIESIINRTINYLQ
                     GGLARTVGVFCIIIAGYLCLARQKFPKEYFVMILVGMGIIFGGSSLYSTLIG"
     gene            complement(4797..5000)
                     /locus_tag="I9760_002387"
     CDS             complement(4797..5000)
                     /locus_tag="I9760_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HAT5044146.1"
                     /translation="MLVLTRKAGQQILIGKGLIQMKVLKVDDDIISIGIKAPQHIDID
                     REEIYLKKLQQEQAESSMQKVAP"
     gene            complement(5013..5375)
                     /locus_tag="I9760_002388"
     CDS             complement(5013..5375)
                     /locus_tag="I9760_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vir protein"
                     /protein_id="HAT5044147.1"
                     /translation="MSSSSKDTFKAERRIDLLFSKFAAFYGHVWRSQFKDEVFLKFAK
                     KEWQEALADFTDVVLTKAILNCREFYELPPTLPQMLYCCRQIRKQESFYVVKDVYQPA
                     NKAVVSSCLQKCKELLAK"
     gene            complement(5341..6207)
                     /locus_tag="I9760_002389"
     CDS             complement(5341..6207)
                     /locus_tag="I9760_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044148.1"
                     /translation="MLDFLINQNELAALCGLPHIQQLTYLRGIRPYMDVKTGIVGIKR
                     RISHQSISEQLYVEPHQGIKSQNFSRDQVRRSVSGLVRAGLIEVQSEGMHLILKCLLA
                     SPHYSVRNKAAINPPQKATTKPRVESLDNTGLSEVEALKADTVESQKAAIPHKDNYYI
                     YLLSQFEKFWSLYPEKKSKNNAQAVFEELKPNAELCHSIINALTAQINHRDTMKLQGT
                     WVPPWKYPANWLAQRCWEDELTDALQEKHHAEHTKNTRKRHTTEDLFCPPCDVEDREY
                     TNVIQLQRYLQS"
     gene            6364..7044
                     /locus_tag="I9760_002390"
     CDS             6364..7044
                     /locus_tag="I9760_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT5044149.1"
                     /translation="MSIKEKIGLRIKQERMSKKLTMKALAELTDNLNISRINNYERGE
                     RTPGPEEIKQLARALEVSPSFLMCLSDDRQGSFKTPGLGGLLPILDYKSACYPALVIQ
                     RIKEESYSEKLDLIPISSTVQKRIGKNAFALAVKDESMAPEFKFDDILIVDPDTQPNP
                     GDYIVARLENEPEIIIRKFKQLSVSKEKPEFELVAINNDWPDVKISKELNEIFIGSVI
                     SLFRTIKR"
     gene            complement(7137..7526)
                     /locus_tag="I9760_002391"
     CDS             complement(7137..7526)
                     /locus_tag="I9760_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019217641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044150.1"
                     /translation="MIDIKMPDFNRIEMAFTKHCMQSGWSSKYNFPCCPKEIEENSLE
                     TYFNNLKVGSVFAFNDHSPNLIILKVAQGENNSSILVMCEREGTLCEWEGFLPWSIIE
                     ITFENKLFVHSNLGSYFEKNDADKHFA"
     gene            7614..8126
                     /locus_tag="I9760_002392"
     CDS             7614..8126
                     /locus_tag="I9760_002392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HAT5044151.1"
                     /translation="MEKTLHDYFCINTGEELKFYSAKESSFLVEAANFHIDRGNGIDA
                     PNTLPELDAIIYECMDEYFKNGLTDYLLNKLNEILINVKIQCLVENMENKLSAIHVAY
                     IPKKPSPVVFGAYMFSHVTSLSGLEGLKRCYNKGCLKFFIGRSNAKWCSSSCGSKYRV
                     NKMRKKKKVS"
BASE COUNT         2421 a         1663 c         1759 g         2522 t
ORIGIN      
        1 atcgtgacgc tccccgataa aaacaccatc atgcctgcaa aataaggtgt gagttttcga
       61 agtgagcctg cttcaaataa atccgaacct aatttgagga tttcgtttaa tacattttgt
      121 aacccctgat tgattgagcc gcttgaagga tttttttgaa cggcctgcat taattgagtt
      181 gcgatggatt cactacccga tacaaacaaa tcgcgcatat aagacgaaaa caaatcccag
      241 ttcatggcca tcatgtaaat aagtcccgcc cgcatactga atcgaaacaa ttcctgctgg
      301 ggtcgctcga taacacccaa tgcaatccca tagcctttca aaatgatgta tagaacaatc
      361 atggcgccca atggggcttt aagtagtgag gccagtgccg tgtagccatc aaaaacaaaa
      421 tgattggtca gcttatcgat ttcaccggca agctggatga tgaaattatc aaactgtagt
      481 gtgctcatga tttaggttct cgaaacaaag tgaggccaaa tggtggctgc tgtttcggca
      541 taggtatctg ccagagtttc aaaaagaccg tggtctgtta aatcatccag ggttatcttt
      601 tgaaaacctg agtaatgacc ttctttggca aaggcaatca tgccttggta tagctcatca
      661 catcggattt catggcttgt tttgccttcc atgcattcca gataggcgaa gccgtaagca
      721 tctttgtgaa tggcaaaatg caatcgcttc tcagcattgg ccgaggcatt aatcaaaaac
      781 gtaacactga tcttggcggg ttgcgttttt aatggatttt cgtgaagaca ggcacttagt
      841 gttgcagcca ttaaaacaag cattagacct tttatgagtg tgttcatttt gtaagctcag
      901 taatgggttc atcgttaatg ggatactgag ggcaatactg cccaaagtga tggcagggag
      961 aatccaaatc ggggggcttt gaacagccgc tcagtaatga gatcatcaat acactcaaaa
     1021 gccatttcat gtggttactc cttgtcactt gggtaatcga ttaaactttg ccatttcagc
     1081 cctgtcttgg agcgcagata aattttcctg tgctgcttgc tggctgatta acgcctgaag
     1141 tcttaagctc attaattgaa taaacccgat ttcggtaatg aggcgggaat ttaaatcaat
     1201 agctcccttg gtgttgggtg ttttttcaat ttgttgcgag agcttatgga gggcttccat
     1261 atgcttgttg atttcgttat agctttcggt ggtttgcacc atcaccgcac gattgacggc
     1321 cttatcttct ttaaagcgag cggcattggc aggagtagtg aaacgcgcat aactgttttc
     1381 atccagtgcg ggatggttct tttcataggc atccatgagt gcttgatatc gttgttgatt
     1441 gccaccagag aggttttgca atgcatcctg ccaagtgctt acaggggatt gccagctttg
     1501 taatgaatcg agactattat taaaatctcc aaatccataa tgtccagagt tcatggattt
     1561 aagttgattc aggttatcaa gttggttttt cgctgtttgg taggtatcgg ataaaacgcg
     1621 gtattgctct ttgagctcac ctaattgttt catcgctaat acaatcaact gcgcatcttc
     1681 gacccctaaa atatccgcat gactggcgct gctgataccc attgctgcaa ttaagaaaca
     1741 ggctgctctt ttcatttttt gctcctttca taaaagacag gaagccaaag ttctgggtca
     1801 tgaccgacct cttgcatgat gtcatccagc aatttaaccg attgagtatt ggcagaaagc
     1861 accctgatgt attggcttag accgcttaaa tcgagtttgc acaaaaaggc tttgtggtct
     1921 tgtttataaa gaaaaatgcg tgattctggc gtgttctctt taatcgcctc aaattgagct
     1981 tcggtcagct ttaaatgctc catgtaagtt tctttatctg caagcggatt aggaaaggca
     2041 atcaaggtgg ccaggttatc cagaacaatg tgtttaatgg gggaatcagt aatcgttttt
     2101 ggggattggg tatcaaaaat aaaatgcccg tttttctttc gaatcgtagg cagccattcc
     2161 cgaagtgctt tttcccaaaa aggagatgca aagagctgcc atgcttcggc gataataaaa
     2221 gaagttaacc gaccatcgag gcattggcgc atacgatgca gcaaatacaa atagacaggc
     2281 gttgcaataa cgctgtgcac ttggtccatc atgtaggtca catcaaatcc caccttgtca
     2341 aaatcaagat ttaaggcatc atttgggtta tcaaacagcc aatgaaactc gccatcaatt
     2401 ctggagtcat tctgctttaa ccatcttttt aaatgtggcc aacgtggaaa atcaacaggt
     2461 agaaattggc tcaagtgact tagcgttctg aactccggtg caagttgttc aaagctgtaa
     2521 tcgatacagt cattgatgat ttcagcaatt tggcttggga taacacattc gctttcttct
     2581 tgtaccaagg ttgcaagcca taatttcaga aaagagcgat tttctgatgt gtctgggagt
     2641 tgcaaaggat tcatagcgat tggattggat ggctctattt tgatgtaaga gctgttaccg
     2701 gaagctaaaa cataaatttt ggaggactca tccctatcaa tgaaaaaact tcttccacca
     2761 aaacgcccca tttgtgcatc aaggaaattc accagtgtgg tttttcctgc attgtttcct
     2821 ccgaaaatgg cggcgtgtcc tttggagggg ttggttttag agcctcgtgc atggtagttg
     2881 aaatagactg gcgttttcga tggggtttcc agaagcgtaa ccgcgccacc taaaaagttc
     2941 tcattggaaa agcccgtttg ggtgttatgt aatgggcaaa agtccacaaa gttttgagag
     3001 gtaatcagag aggctcgtgt tataaaatgc tggttgcatg gcaattggct ccagaaggct
     3061 ggctcttgtc ctaacgtttc tttcaccacc acaattcctg aagccccgta gcgtttggtg
     3121 gcttccagta tggctgtatc caataatggc ttgttgggtg ccaataacat taccgtgtga
     3181 tgatgtgcgc ccagcaacag ggtttcactg gcaattccat cttccaaatc gcttaatgcc
     3241 gtggtttggc ttaatgcctt gtcctccgca cttaagagct tggagcgttt tttagaaatc
     3301 atattcaagg cactatctcg cccaatgggg gcaaatgtat gtgtggcaat aaattcacaa
     3361 tccagagata acatggagtc caagaggatg ctggcggtgt tggtgggata ttttttgagt
     3421 gacagcatcg caccaaacac gcaatccttt tgagtattgc cttgaaactg gatgcactcc
     3481 ccaaataaaa gctgacaact acttaaatat tggccgagat gtccttcagg gtatttggct
     3541 tcctgtctga atgactgagg gatcgagttt gctattggag ggctgtacac aggatgtttg
     3601 aatgaaattg tttgacctgc attcaccacc aacccaagga actgaagcag ctcgctaaaa
     3661 cctaatgctt catcctgctt gccaagtatc gttgccccga aaggattgag gtttgcttgt
     3721 aactgctcaa caacacgatt taagatttgg atgttttgtt ccctgtggtg ttcagacaac
     3781 tcactattgc ccttcatcga gaggcttttt gcccactgca accaagatcc ggtcttactg
     3841 gagtcatctc ctttaaggac aatggtgatg tacagagtat ttttatacag gtttttatct
     3901 ttaaagcgtt gttggtaatg ctcatccaga tccttggcaa agcctggctt aaattcccca
     3961 atcaaagggc atgatatttt ttgtcggtgt gtggtcacat aaataatgaa acgcgaatcc
     4021 aaactcacta atgcctgatg gagcaagaag ctttggtgat ttagagtctc agcttcttcg
     4081 atttcaaaag ccgcaccatt caccttaatc accgacccaa ccaagcctga ttgcgattca
     4141 aaaatgctgg ggctattaag atgcgttacg ggaaacaaat ctgacacttt tgcttcctga
     4201 taaacgtgct tcaatgtctt agaaaggttc ataagcgcga accctccata gtgatgtatt
     4261 gggagcttct ggcaataaga aacgctttgc gcagatggta aaaaaatggg tgtcgtatcg
     4321 acacactagc catccaaaaa catgcagtgg aataaaaatc agcccgtaca gtggagagaa
     4381 cccaataaat gcgcacatcg agacaatgag gtttaacatg tggtattcaa aggtcacccc
     4441 agccgtcatt gccggacggg ttagcgcatt aaatatgggg cttatttcca aatcgctcac
     4501 aagaatctcc tatccaatga gtgttgaata cagggaagag ccgccaaaaa tgatccccat
     4561 tcctacaaga atcatcacaa aatattcttt aggaaatttt tgacgtgcca aacacaggta
     4621 acctgcaatg atgatgcaaa aaacgcccac ggttctggca agcccaccct gaaggtaatt
     4681 gatggttctg ttgatgatgc tttctattga ttgggcatgt gacagcgttg gcagcaaagt
     4741 gcaaactgct attaaataga ggttgccaag ggattggctc catcgtttta atttgctcat
     4801 ggcgccacct tttgcatgga agattcagcc tgctcctgtt ggagtttttt caaataaatc
     4861 tcttcgcggt caatatcgat atgctggggt gctttaatgc caatgctgat gatgtcatca
     4921 tcgaccttga gtaccttcat ttgaatcaaa cccttaccga ttaaaatctg ttgccctgct
     4981 tttcgtgtta aaaccaacat ggtgttctcc ggttattttg ctaaaagttc cttgcacttc
     5041 tgcaaacagg aacttacaac ggctttgttt gcaggttgat acacatcctt gaccacataa
     5101 aacgactctt gtttcctaat ttgtctgcag caatacaaca tttgcggcag cgtaggcggt
     5161 aactcataaa actcacggca atttaaaatc gccttggtca gtaccacatc ggtaaaatcc
     5221 gctaatgctt cttgccattc ttttttggca aatttcaaaa acacttcatc cttaaactga
     5281 cttctccaaa catggccata aaaggctgca aacttggaga acagcaaatc aatacgtctc
     5341 tcagctttga aggtatcttt ggagctggat gacattggtg tactctcggt cttcgacatc
     5401 acagggtggg cagaacaggt cttctgtggt gtgacgttta cgagtatttt ttgtatgctc
     5461 tgcatggtgc ttctcctgta atgcatccgt taattcatcc tcccagcagc gttgagcaag
     5521 ccaatttgct gggtatttcc aaggtggcac ccaagtgcct tgtagtttca tggtgtcacg
     5581 atgattaatt tgggctgtca gtgcgttgat gatggagtgg cacaattcag cattaggttt
     5641 aagctcttca aatacagctt gtgcgttgtt ctttgatttt ttctcgggat aaagcgacca
     5701 aaatttttcg aattgcgata ataaataaat ataataatta tctttatgag gtatggcggc
     5761 tttttgggat tctactgtgt cggcttttag tgcctcaacc tcggataatc cagtattatc
     5821 aagggattcc acgcgcggct ttgtggtggc tttctgtggc gggtttatgg cggctttatt
     5881 tcggacagaa taatgcggtg aagccaaaag gcattttaaa atcagatgca tcccttcgga
     5941 ttgcacttca attaagcccg cacgaaccaa tcctgaaacc gaacgccgaa cctgatcacg
     6001 cgaaaaattc tggcttttta tgccttgatg cggttcaaca taaagctgct cagaaataga
     6061 ctgatgactg atgcgccttt tgatgccgac tattccggtt ttaacgtcca tataaggtcg
     6121 tatccctctt aaataagtaa gctgctggat gtgaggtaac ccacataatg cggccaattc
     6181 attttggtta attaagaaat ccaacatccc cgcacttctc catatgccaa gtccaaatta
     6241 caataaactc tttatgagta aaaatacacg aaacgagtaa ttaaagttta aagttaacac
     6301 acattgagaa tgagtcaata tctgttatca tattttttaa gaatattttt gtatgccaac
     6361 cgaatgagta ttaaagaaaa aattggatta aggattaaac aagagcgcat gagcaaaaag
     6421 ctcactatga aagcacttgc tgagctaaca gataacctaa atatctctcg aataaacaat
     6481 tatgagaggg gagagcgaac ccccgggcca gaggaaatta aacagctagc aagggcttta
     6541 gaagtttctc cttcgttttt aatgtgtctc tctgatgatc gccaaggtag ttttaaaacc
     6601 ccaggcttag gtggtctgtt gcctatatta gattataaaa gtgcttgtta tcctgctcta
     6661 gtaattcaaa gaataaaaga agagtcttac tctgaaaaat tagatttaat tccgattagt
     6721 tctactgttc aaaagcgcat agggaaaaac gcttttgcgc ttgcagttaa agatgagagt
     6781 atggctcctg agtttaaatt tgatgatatt ttaattgtcg atcccgatac tcagccaaat
     6841 cccggtgatt atatcgttgc cagattggaa aatgagcctg aaataattat tcgcaagttt
     6901 aaacagttat cagtatccaa agaaaaacct gagtttgaat tagttgcaat caataatgat
     6961 tggccggatg ttaaaatttc aaaggaattg aatgaaattt ttattggctc agttatttca
     7021 ttatttagaa ctattaaacg ttaaatcctt agtccaaaaa catttagggc tagatatata
     7081 aagcagtttt tcctgtaacg gttagcaatt gcctacttgc attctaaaac catgcctcat
     7141 gcgaaatgtt tgtcagcatc attcttctca aaataggaac ctaaatttga atgaacaaat
     7201 agcttattct caaaggtgat ttcgataatg ctccatggta aaaatccctc ccactcacac
     7261 agagtcccct ctctttcaca cattactaaa attgaagagt tattttcccc ctgtgcaact
     7321 tttagtatta ttaggtttgg ggaatgatcg ttaaaggcaa ataccgaccc aacttttaaa
     7381 ttgttgaaat acgtttcaag tgaattttcc tcaatttctt tagggcaaca aggaaagtta
     7441 tatttagaac tccatcctga ctgcatgcag tgctttgtaa aagccatttc tatacgatta
     7501 aaatcaggca ttttaatatc tatcatttct tatccttatt cttttagggt tgaaaactac
     7561 aatgtaacgg gatataaaca ttaaatgcgt tacaccttca agagaggatt taagtggaaa
     7621 aaacgttaca tgactatttc tgtattaata caggtgagga actcaagttt tacagcgcta
     7681 aagaatcatc atttttagta gaagcagcaa attttcatat agacagaggg aatggaatcg
     7741 atgctccaaa taccttgcct gagctggatg ctatcattta tgaatgcatg gatgagtatt
     7801 ttaaaaatgg tcttactgat tatttattaa ataagctaaa tgaaattctt ataaatgtaa
     7861 aaattcaatg cctcgtagaa aatatggaaa ataaactctc tgccattcat gttgcttata
     7921 tacccaagaa gccatctccc gtagttttcg gagcttacat gttctctcat gtaacctcac
     7981 tgagtggtct tgaaggattg aaaagatgtt ataacaaagg ttgtcttaag tttttcatag
     8041 gtcgatcaaa tgccaaatgg tgttcaagct catgtggttc taagtatagg gtaaataaaa
     8101 tgaggaaaaa aaagaaagtt tcataattgc attgcagtaa caggaggaac agttttgagt
     8161 atttagcgca taaatttagc ttaggagatg atagcccata ttcagatttt ctgttatcta
     8221 cgtacacaca tcagactctt ctaagttcaa aacgcggtga agtatgccct atgaaaaatg
     8281 attcttaatt tattttttat aaagtcgata agtcgactat atataatgag gtttgctgca
     8341 tgaaaaaaag tataaaaaaa gtctt
//
LOCUS       DACTJE010000145         8284 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.145, whole genome shotgun sequence.
ACCESSION   DACTJE010000145 DACTJE010000000
VERSION     DACTJE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.145"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            138..1001
                     /locus_tag="I9760_002393"
     CDS             138..1001
                     /locus_tag="I9760_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAT5044152.1"
                     /translation="MTDIAVIINNRAKNAHRMEDYLSELRAHDIDYQLYKADPQQLEK
                     NIQHCCSKYPLLLIGGGDGTVRSAAQWCSNTSVILGVLPLGTMNHFSKELNLPSTTEE
                     LIAAIVEKTTTTIDVAEVNGHIFINNSSIGFYSRLAKNRDYYTNFYNKWLTYIPSFIR
                     ALSYHPSYALLIKNKELDISIRTSFFMVSNNCYTYEFPLKFTRESFQNEQLGIYYYKR
                     SKLRLFKFLFDFLTDKEHFEIIQTRLPLEVDVVHKNKITISLDGDTLAIAPPLYYKIL
                     PKSLKLLTKKI"
     gene            998..1807
                     /locus_tag="I9760_002394"
     CDS             998..1807
                     /locus_tag="I9760_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAT5044153.1"
                     /translation="MKIIHISDLHFGMHQPKVLNAFLQETALDKPDIILISGDLTQRG
                     LSYQYRELCSFINQLPAQTLSVPGNHDIPFYNVPARFIFPFRQYKRYINPDVTTTFEN
                     DCVRILGVNSVNPLQLKDGKLSHETLNRIKRYFKPDDEKLNLLFFHHNFDYMEGLHKP
                     LANAQTLLSFLKQSTIHLVCTGHLHYAHLSLIAKNNGYSCLALHAGSLLCPRSKDNLN
                     SYYVIEANQKKCRITWRVFDQEHFTARSIYFIDFNKKNASFEHLNPVTDDA"
     gene            complement(1861..4143)
                     /gene="legC4"
                     /locus_tag="I9760_002395"
     CDS             complement(1861..4143)
                     /gene="legC4"
                     /locus_tag="I9760_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC4"
                     /protein_id="HAT5044154.1"
                     /translation="MIHYVSLIHFNELFEIMKPNLFYFVLTHILAESPNLKLLDEITP
                     VHDITLKDKKYYRLELPLANIAAEGFRLHDAHISLYEKTDPHNPNLGLSHFTAIFHDQ
                     NGQTYRMHLFLNRFDALACPATWELLDDDEQYVKVPPPENLEYFIHSVWQLGLPCLQL
                     LRKKQKEIEAKLLADYHRLNEETTSLSKDLDKNRAVYLEKLDELIKTTKSLSQISESN
                     HWLREAIYLGKTHKYVSSMPEPILEPVKKSDEEHKKDDKAEVVALSPKQGATEPKISR
                     GNIGLSQSTLFSKSAKAKRPVDVKMERDISKIKKLFAQLLKTEDKKIKAVLLIDLHWK
                     IKEINLETEELLTKEQTQALNEIEGRANKEAKSLLERALFAGEFEYAQTLSPYYPLIN
                     NDLMVLALTQRKADLLSFLVTKVGLPINSYPIKTKAQTYSNAVEYCFSEHSESLVDCF
                     SVLIKNGASLMQPVGLHKLPLAHLLLSEIPRHPLHAALEQNKNLTLNNKQFYAHLINA
                     LKSCLLSGDIEGDQKIALEESIVRYEHLKINVKNSSSLLSPQNQALADEISDITKKLL
                     PDAVAEAIENDEEISREKAIADKEYKELIRKVKAFYHKTGKNFAFQSLINSANQELKK
                     ELLEIDFNIDISFSELKESILENLSKERLMFSYCSELIDVQRTIMQISQGASRKNKNL
                     KKLNARHAELIELLTDLSQNTPQSMIKEANEFQDSFSQLQDLLNGLNQLEGSFGMLAG
                     VLNQFLEGSTSESESFDIKL"
     gene            complement(4534..5109)
                     /locus_tag="I9760_002396"
     CDS             complement(4534..5109)
                     /locus_tag="I9760_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044155.1"
                     /translation="MLPSSKDAQLCTNAYNKLWHIFDSVKADYLGDPAAQAAIFNVEF
                     PKVLAEDFSLTINNLPVEPNSSETITVNVQGLPQVISMALEQITSWGQHHIAGPFSFK
                     LLSESLPYKTRVYLMTTRDIDYTNSPENNGCAAFLAQKNMVCTVKNTGLTTRKAILNS
                     MNSHVVTTYIIPAPNCSLWIAPPVEDFPTEA"
     gene            complement(5410..6699)
                     /locus_tag="I9760_002397"
     CDS             complement(5410..6699)
                     /locus_tag="I9760_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAT5044156.1"
                     /translation="MSFYIQLASLSIIILYVLLLFRNVDPLVATAVCVGLGFLWNIST
                     PIDIGNAMADALGSFMALVGFIIMLGRGLGEVLTHTQVSHTLVHQIVYGVGVNTQRRA
                     KIGIVLSSFIIVGLLGTLAGGLAILAPSLRPIAGSVGLSRPSLAVLMQASAEEALILG
                     PFAPPVVTLLGVTGLSYGTVFLYASLPIAVVTLITTWFMASRLQKLYINEPCEDENST
                     EPFTPNRQQKRVTLIFLISFLFCVVYGLFTQAKTSYVIFVMLFLALITGLSCRLGLKQ
                     IFNLLFEGMQKSLHLFFLFILFDPFMALIHQAGGFNALTELLTPLINLGGKPVLSILI
                     GFTGAFGMPGAAEATIKMLHQLFSPSVLQMQLPLITFALCMLFATRVTNYAYPGANMF
                     AAMGFAGSENVKAMIQNGLVVTGVQVAFLIVYSLFIP"
     gene            complement(6699..7802)
                     /locus_tag="I9760_002398"
     CDS             complement(6699..7802)
                     /locus_tag="I9760_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HAT5044157.1"
                     /translation="MNIKEIHIAELTIPLSRPFITAVRRTECVDDVVVLIKTDCGKIG
                     YGSAASTPAITGDSKESIIAAIKSALGPPLIGHNIAEFNLLLQMTDEAIGGNSSAKAA
                     IDIALHDLFAQYCGLPLYRLLGGNTNCIDSCITISVKEVDEMVSDAVDLIQRGHKTLK
                     IKLGLNPVDDINRIRAIRQAVGNDITLLADANQGWSYEEALIAITAFKKENLNVPLVE
                     QPVNARNLPDLKTISDQVGCAIIADEACFSPEDALNIAKINACDGVNIKLMKSGGIEK
                     AQSIYHIAQAAKMQIMVGCMLESPIGVAAIASFALSKPDISYADLDPIFLIRENYILG
                     GAQSLSNKIILADKPGLGIEGITQGLNLIGVIR"
     gene            8105..>8284
                     /locus_tag="I9760_002399"
     CDS             8105..>8284
                     /locus_tag="I9760_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbhA"
                     /protein_id="HAT5044158.1"
                     /translation="MLFKLNKSSSHKPEQISRSYHRLMLQLKTWETNHYTPDLNEEYF
                     RTLDIETRNVQIFYND"
BASE COUNT         2635 a         1645 c         1452 g         2552 t
ORIGIN      
        1 ttgaactctc aaaaaaatct gtctttcatg cctacacaag ttaataaaat taaaatgccc
       61 gacggcatcc catgttgtct tactataatt tttgaatgta acctaaactt taagtaagca
      121 ggacattggg gagcttcatg acagacattg cggttattat caataatcga gctaaaaatg
      181 cacaccgaat ggaagattat ttatccgaac tcagagcgca tgacattgat tatcaattat
      241 acaaagcaga ccctcaacaa ttggaaaaga atattcagca ttgttgctca aaataccctt
      301 tactattaat cgggggtgga gacggcactg ttcggagtgc tgctcaatgg tgcagcaata
      361 cgtctgttat tcttggtgtg cttcctttag gaactatgaa ccatttttcc aaagagttaa
      421 atttaccttc aactactgag gaattaatag cagcaatcgt ggaaaaaaca acgactacaa
      481 ttgacgtagc agaagtaaat ggccatatat ttattaacaa ctcgtctatc ggcttctact
      541 caagactggc aaaaaaccgt gattattata caaattttta taataaatgg cttacttaca
      601 ttcccagttt tattcgggct ttgtcatacc acccctctta tgccttatta ataaaaaata
      661 aggaacttga tatatcaata cgcacttcct tttttatggt aagcaacaat tgctatacct
      721 atgaatttcc tctcaaattt acccgggaaa gttttcaaaa tgagcagctg ggcatttatt
      781 attacaaacg tagtaaattg cgtttattca aatttttatt tgatttctta acggataagg
      841 agcattttga aataatacag acaagattac ctctggaggt tgatgttgtt cataaaaata
      901 aaattaccat ctctttagat ggggatacgc tggctatagc accccctctt tattataaaa
      961 ttttacctaa atcactgaaa ttactgacta aaaaaatatg aaaattatcc atatttccga
     1021 tttgcatttt ggcatgcatc aacctaaagt tttaaatgcg tttcttcagg aaaccgccct
     1081 cgataaacca gatatcattc ttatatcagg agatttaact caacgtggac tctcgtatca
     1141 gtaccgggag ctttgctcat ttataaatca actgccagct caaacgcttt ctgttcccgg
     1201 taatcatgat attccctttt ataatgttcc agcacgtttt atattccctt ttcgccagta
     1261 caaacgctac ataaacccgg atgtaaccac cacctttgaa aacgattgtg tgcgtatttt
     1321 gggggttaat agtgtcaacc ccttgcaatt aaaggatgga aaattatctc atgaaacatt
     1381 aaatagaatt aaacgatatt ttaaaccgga cgatgaaaaa ttaaacttgc tcttctttca
     1441 tcataatttt gattatatgg aaggattaca taagccacta gcgaatgcac aaacgctttt
     1501 aagcttttta aagcaaagta ccattcattt ggtttgcaca ggtcatctac actatgctca
     1561 tttaagttta attgctaaaa ataatggcta ctcctgtctg gcgctgcatg ccggctcatt
     1621 actgtgccca cgcagcaaag ataatttaaa cagctattat gtgattgaag cgaatcagaa
     1681 aaagtgccgc attacctggc gtgtttttga tcaagaacac tttactgctc gctccatcta
     1741 ttttattgat tttaataaaa aaaatgcgtc atttgagcac ctaaatccag tcacagatga
     1801 tgcttgatgc acaggactac actgtatttc gcttgtcaga acactagtat gccaattatc
     1861 ttatagctta atatcaaaag attctgattc cgatgttgag ccctcaagga attgatttaa
     1921 tactccagca agcatgccaa aagagccctc caattgattc aagccattca ataaatcttg
     1981 aagctgacta aaagaatctt gaaattcatt ggcttctttt atcatagatt gaggcgtatt
     2041 ttggcttaaa tctgtgagca attcgataag ctcagcatgt cgcgcattga gttttttcag
     2101 gtttttattc tttctgctcg ctccctgcga tatttgcatg attgttcttt gaacgtctat
     2161 taattcactg caatagctga acattaatct ttccttgctt aagttttcca gtatggattc
     2221 cttaagctcg ctaaagctaa tgtcgatatt aaagtcaatt tctaataatt ctttcttcaa
     2281 ttcctgattg gctgaattaa ttaaggattg aaaagcaaag ttctttccgg ttttatgata
     2341 aaatgccttc accttacgaa tcaattcttt atattcttta tcagcgattg ctttttctct
     2401 tgatatttcc tcatcattct caattgcttc agccacagcg tcaggcaata attttttggt
     2461 aatatcgctt atttcatcag ccaatgcctg gttttgagga ctcaaaagtg aggatgagtt
     2521 ttttacatta atctttaagt gctcgtatcg cacaatggac tcttccaggg caattttctg
     2581 gtcaccttcg atatcaccag ataataaaca tgattttagt gcattaatta aatgagcata
     2641 aaattgcttg ttattgagag ttaaattttt gttttgttct aacgcggcgt gcaaagggtg
     2701 cctggggatt tcagacaaaa gaagatgggc taaaggtaat ttatgtaagc caacaggctg
     2761 catcaaactt gcgccatttt ttattaacac gctaaagcag tctaccaacg attcactatg
     2821 ttctgagaag caatactcaa cggcattgga atacgtttgc gcttttgttt taattggata
     2881 actattgata ggcaaaccaa ctttagtgac taaaaagctt aaaagatcag cttttctctg
     2941 agtaagcgca agaaccatta gatcattatt aatcaaagga taataaggcg acagggtttg
     3001 agcgtattca aactctccag caaacaaagc tctttccagg agtgacttgg cttctttatt
     3061 tgcacggcct tcgatttcat ttagggcttg tgtttgctct tttgtcagta actcttccgt
     3121 ttccaaattt atttctttga ttttccaatg taaatcaatt aaaagaactg ctttaatttt
     3181 tttatcttcc gtctttaata gttgagcaaa caattttttt atcttgctaa tatcgcgctc
     3241 cattttcaca tccacagggc gtttcgcttt ggctgatttg gaaaataagg tcgattgaga
     3301 aagcccgata ttgcctctgc tgattttggg ctccgtagcc ccttgctttg gagacaaggc
     3361 aacaacctct gctttgtcat cttttttatg ctcttcatca ctttttttaa caggctcaag
     3421 aattggttcc ggcatagagg atacgtactt gtgtgttttc cccaggtaaa ttgcttctct
     3481 caaccaatga ttactttcag aaatttggct taatgattta gtcgtcttaa ttaattcatc
     3541 aagcttttcc aaatagaccg ctctgttttt atctaaatcc ttacttagtg aagtcgtttc
     3601 ctcgtttaat ctgtggtaat cagcaagcaa tttagcttct atttcctttt gcttttttcg
     3661 caacaattgc aaacaaggca ggcctagctg ccatacagaa tgaatgaaat attccagatt
     3721 ttctggtggg ggaactttga catattgctc atcatcatcc aataactccc aggtagctgg
     3781 gcatgccaga gcgtcgaaac gattcaaaaa caaatgcatt cgataggttt gaccattttg
     3841 atcatggaag atcgcagtaa aatgcgaaag tcctaaattt ggattatggg gatctgtctt
     3901 ctcataaaga ctaatatgcg cgtcatgcag cctgaatcct tctgcagcaa tattagccaa
     3961 gggtaattct aagcgataat acttcttatc ttttaaggta atatcatgga ccggtgtaat
     4021 ctcatctaat aatttaagat ttggggattc agctaaaata tgcgtcaata caaagtaaaa
     4081 caaattgggt ttcataatct caaacagctc attgaaatga atcaaggata cataatgaat
     4141 caaatttgtt caacttgttt tttaaatcca agagctattg tccacaacat ggaatgacat
     4201 cctgaataac aacaagcaaa tgccggtaaa tgctgtatcg attcaataaa aagccgacag
     4261 tatgcagtta gaaagaatca ggacttacga aaacccccaa gatcaaggca aaaaatattt
     4321 ttaatgaggg ggtttagcta aactaaatga ccgaattaaa aatttttttt aacaaagaga
     4381 ttgggatttt tcgtaagtcc tgagaatgta aaacctgtgt ttatcggatc ctttcttaac
     4441 tcattaaata tcccaaagtg acagatttta cagaattttg gggaaattaa ataagtggtt
     4501 tttcgggaag agaactcatt tcattactgt aaattatgct tcggtaggaa aatcttcaac
     4561 cgggggagct atccatagac tgcaatttgg agcggggata atataagttg ttacaacgtg
     4621 agagttcata ctattaagaa tagcttttct agtcgtaagc ccagtatttt taacagtgca
     4681 aaccatattt ttttgtgcca ggaaagctgc gcaaccatta ttttccggag aattggtgta
     4741 atcaatatct cgggttgtca tgagataaac ccgtgttttg tagggtaatg attcagagag
     4801 gagtttaaaa ctgaaaggac cagctatatg gtgttgtccc catgaagtaa tttgctctaa
     4861 agccatggaa atcacttgag gaagtccttg cacattcaca gtaattgttt cagaactatt
     4921 gggctcaaca ggtaaattat taatggtcag ggagaaatct tcagccagta ctttgggaaa
     4981 ttccacatta aatatagcag cttgagcagc tggatcacct aggtaatcag ccttcacact
     5041 gtcaaaaata tgccaaagtt tattataagc attggtacaa agctgagcat cttttgaact
     5101 gggaagcacg cttgcgaaaa ctggattgat atcggcaaca attaagccta taaaaaaaat
     5161 atatatattt catgtaacta actccttgtt atctaagttg aattaaattt gacttgatct
     5221 gatacagcct attgaaaaaa aaagttttca ttttaaaaca tattaaatac aaatttgact
     5281 tgctcaattt agctttggca gaaattttta aacacttttc tttaccagct tctgcatcta
     5341 tataagccct aagcatagcc ctgtgtggtg caaactcatt gatttaaagg attaaagcga
     5401 tcacaaacat cagggtatga ataaactata aacaattaaa aatgcaacct ggacgccagt
     5461 cactacaagg ccattttgta tcatggcttt gacattctca cttccagcaa aacccattgc
     5521 cgcgaacata ttggcgccag ggtaagcgta atttgtgacg cgagttgcaa agagcatgca
     5581 caaagcaaaa gtgataagag gcaattgcat ctggaggaca gaagggctaa agagctggtg
     5641 aagcattttg atggttgctt ctgcagcgcc tggcatacca aaagcgccgg taaatcctat
     5701 taagatcgat aatactggtt tccctcccag gttaattaat ggtgttaata actcagttaa
     5761 tgcattaaat cctccagctt gatggattag tgccataaaa ggatcaaata aaataaacaa
     5821 aaagaagaga tgcaagcttt tttgcattcc ttcaaataac aggttaaaaa tttgtttcaa
     5881 accgagtcga caggataatc cggtaatcag cgccagaaat aacatgacaa agatcacata
     5941 agaggttttc gcttgagtaa ataagccata gacaacacaa aacaaaaagg aaatcagaaa
     6001 aattaatgta accctttttt gttgtctatt gggagtaaag ggctcagtgc tgttctcgtc
     6061 ttcgcagggc tcattgatgt ataatttttg taatcgtgaa gccataaacc aggtggtaat
     6121 taaagtcact acagcaattg ggagcgaagc gtaaagaaat actgtcccat aacttaatcc
     6181 agtgacacca agcaaggtta caaccggggg agcaaaaggc cccagaatta aagcctcttc
     6241 agctgaagct tgcatgagta ccgccaaact gggacgtgac aagcctactg accctgcaat
     6301 aggacgcaaa ctgggggcca ggatagctaa acctcccgct aatgttccca gcaaaccaac
     6361 aataataaag gatgagagga ctataccaat tttagcgcgt cgctgagtat taaccccgac
     6421 tccataaaca atttggtgca ctaaagtatg acttacctgg gtatgcgtta aaacttcgcc
     6481 caaccctcga cctaacatga tgataaaacc aaccaaagcc ataaatgaac ccaaggcatc
     6541 agccatggca ttccctatgt caattggggt gcttatattc cacaaaaaac ctagcccaac
     6601 gcaaacagct gtcgctacta atggatcaac gttacgaaat aacagcaaaa cataaagaat
     6661 aataatggat aaactggcta gctgaatgta gaaagacatt agcggatcac tccaattaga
     6721 ttcagtccct gtgtgatgcc ttcaatccct aatcccggtt tgtctgccag gataatttta
     6781 ttgctgagac tttgtgcccc accaagaata taattctcac ggatcaaaaa aataggatcg
     6841 agatctgcat aagaaatatc gggcttactt aatgcaaaac tggctattgc agcaactcca
     6901 atcggcgact ccagcataca tccaaccatt atttgcattt tggccgcttg agcaatatgg
     6961 tagatagatt gagctttctc aatgcctcct gatttcatca actttatatt gaccccatca
     7021 caagcattaa tctttgcgat attcaatgca tcttctggtg aaaaacacgc ttcgtcagcg
     7081 ataattgcac aaccaacctg atcactaatc gtctttaagt caggcagatt tcgagcatta
     7141 accggttgct ccaccagagg aacattcaaa ttctcttttt tgaacgcagt aatggcgatt
     7201 aatgcctctt cataagacca tccctgattt gcatctgcta gcagagtaat gtcattgcct
     7261 actgcctgac gaatagctcg aattcgattg atgtcgtcca ctggatttaa tcccaatttg
     7321 attttcaggg ttttgtgccc tcgctgaatt aaatcaaccg catcactcac catctcatca
     7381 acctctttga cacttatcgt aatacacgaa tcgatacaat tggtatttcc tcccaacaat
     7441 ctatagagag gcaaaccaca atattgggca aataaatcat gcaaagcgat atcaatagca
     7501 gccttcgctg acgagttacc tcctatagcc tcatcagtca tttgcagtaa taaattgaat
     7561 tcggcaatat tatgtccgat aagaggaggc cccaaggcac ttttaatagc agcaataatg
     7621 gattccttgc tgtctccagt tatagcaggt gttgatgctg ctgaaccata accaatcttt
     7681 ccacaatccg tttttattaa taccacaaca tcatcaacgc attctgtacg tctcaccgcg
     7741 gtaataaacg gacggcttaa tggaatagtt agttctgcga tatggatttc tttaatattc
     7801 aaacaagcac ctgttcattt attgtttgcg gggtaaccct aatgagatat taaaatatta
     7861 agagttaatt tggcaaaaca tgtttttaaa ttatatctct caccaaataa tattaacttg
     7921 atacccagct tcaatacatc tcttttctat tcttgaatca actcaataaa ataaaccaac
     7981 tatttctgac gaacaccaaa aacagatacg gatttcatgt attcaaaaac aactgtcttt
     8041 tgctatcatc caaatttctt atcatcccca aaatcaatcc atttgtatta acctggagat
     8101 acccatgctt tttaaactaa ataaatccag ctctcacaaa cctgaacaaa ttagcaggag
     8161 ttaccaccgt ttgatgcttc agttgaaaac atgggaaaca aatcactaca caccagactt
     8221 aaatgaagaa tattttagga ctttggatat tgaaacaagg aatgttcaaa tattctacaa
     8281 tgat
//
LOCUS       DACTJE010000146         8259 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.146, whole genome shotgun sequence.
ACCESSION   DACTJE010000146 DACTJE010000000
VERSION     DACTJE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.146"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(143..928)
                     /locus_tag="I9760_002400"
     CDS             complement(143..928)
                     /locus_tag="I9760_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAT5044159.1"
                     /translation="MKFTSLLTAGALASTLVSPAIMAADTASASLSDAQKKEIEKVIH
                     DYLINNPEVLLEASQALQQKQQQNMQQQAQAAIQENAEQVFQGKLTTVGNPKGNVTLV
                     EFFDYQCIHCKKMASTIENLVKKDSGLRVIYKEFPIFGKTSDLASRVALAAGMQGKYQ
                     AMHNALITIDKRLDEKTVMDAAKSIGLDMQKLKKDMDSQEVTDILDANRQLAEKLHLM
                     GTPAFIIGSTPDGQYKKGSEISFIPGATSEQSLRELIKKAAGN"
     gene            complement(1136..3301)
                     /gene="uvrD"
                     /locus_tag="I9760_002401"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1136..3301)
                     /gene="uvrD"
                     /locus_tag="I9760_002401"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAT5044160.1"
                     /translation="MTIAALLKGLNEKQRDAVTSPLGNMLVLAGAGSGKTKVLVSRIA
                     WLIEEQHLSPHAILAVTFTNKAAGEMRSRLSSMLSTPTLGLWVGTFHGLCHRLLRRHY
                     KEANLPEQFHILDSEDQARVIKRVILSLNLDPEQWQVKQAQAFINSKKDEGLRPQHIN
                     ALHYGPAKTLVAIYKAYEDVCQTSGVIDFAELLLRTHELLRDNEEILAHYRERFQAIL
                     VDEFQDTNTIQYAWIRLLAGDHTAVLAVGDDDQSIYGWRGAKVENIQQFVHDFKDTHI
                     IRLEQNYRSTAMILNAANALILNNSTRMGKELWTAGSEGEKILVYSAFNELDEARFVT
                     ERIGMELNQGASADEIAVLYRSNAQSRVLEEALLRAGIAYRIYGGVRFFDRAEIKDTL
                     AYLRLLVNPNDDTAFERVVNFPTRGIGEKTLDEVRQYARAEQCSLWGASKGILQSTGL
                     GQRGSLALAKFIDLIEKLQVVVANKELDEQISDVIQHSGLYAHFSKIKGDKSESRVDN
                     LQELINAAKQFRYEYDEEEELPLVNSFLAHASLESGELQADEHERSVHLMTLHAAKGL
                     EFPIVFLVGMEEGIFPGRQSLEEPGRLEEERRLCYVGMTRAMRKLVLSYAEVRRQYGR
                     EEYHRSSRFLREIPQQFLDEVRVKSRSQWPGTTKSKLPVADEVSGITIGQNVQHAKFG
                     QGVVLAIEGSGAHTRVQVKFSEHGVKWLVLAYANLTECL"
     gene            3522..4481
                     /gene="pip"
                     /locus_tag="I9760_002402"
     CDS             3522..4481
                     /gene="pip"
                     /locus_tag="I9760_002402"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HAT5044161.1"
                     /translation="MHTLYPAIKPYTQHELKVSKLHTLYLEETGNPEGIPVIVLHHGP
                     GGEADPHLRRFFDPQRYRIILFDQRGCGRSIPHLEISENNTQLLMEDIDSIRDFLGLR
                     EFVLFGGGWGSLLALLYAQKFPQQVKALLLHQIFLGRQKDIDWFYKHGASLVYPDYWE
                     EFTNTVPENKLDNIPQYYAECLQGSNELARMAAAKNWALWQARCSSLQPHLYVIDQYS
                     DPHFALALATLESYYIINHYFIEENQVIANAHKIRHIPTYIVHGRFDLVSPLSSAWEL
                     HQAIPASNLRIVRDAGHSDRESGIIDALIYASKDISKQGLDAC"
     gene            4475..4912
                     /locus_tag="I9760_002403"
     CDS             4475..4912
                     /locus_tag="I9760_002403"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAT5044162.1"
                     /translation="MLTVLQRVKEARVDIDGQTVGKINHGLLILCGFEPKDSLENIKR
                     MLDKCINYRIFEDPSGKMNLSLKDVNGGLLLVPQFTLMADTQKGLRPSFSNAASPELG
                     RELFDNLLTLAQKCHQNTQSGCFGANMQVYLCNDGPVTFLLQF"
     gene            5129..5911
                     /locus_tag="I9760_002404"
     CDS             5129..5911
                     /locus_tag="I9760_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HAT5044163.1"
                     /translation="MRLILMFASIMGSVILASCASKGSNPIDPFEPFNRKVHNFNMAF
                     DATVLRPPAKLYQAVVPKLVRKGVNNAFNNLDLFPTIASDILQAEGKWVIKDSWRLFI
                     NSTIGVAGLFDVAEKFGLPPHYNDLGLTLAKWGDKKSPYLVIPFLGPSTLRDGAGWLF
                     QFALWSPYVYIDDAGLVYGLIGLRYIDLRTQFFDSEPLLNEALDKYTFIRDAYLQHRN
                     YLIAGTEQDTGTSFVEDKKANKTAMQESTASNNQLGSDYVDE"
     gene            complement(6020..6979)
                     /gene="gshB"
                     /locus_tag="I9760_002405"
     CDS             complement(6020..6979)
                     /gene="gshB"
                     /locus_tag="I9760_002405"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAT5044164.1"
                     /translation="MKLAVLMDPLDQIKPYKDSTVAMIKSAQGLGWSCAYFTLNDLYC
                     CDGGAYARVHEIHIGDIESLSWVKTIDLGNKPLRDFDVILMRKDPPFDMEYIYATYAL
                     DLAEQEGVLVANKPQSLRDANEKFFTLNFPHCCPPTFVSRDARQLKEFWQIHQQVIFK
                     PLEGMGGNSVFYVDKEGKNLSVILEVLTKGQTKCIMAQRYIPDITVSGDKRILLINGE
                     PVPFALARIPAKGDLRGNLAAGARGEVVAITERDKWICEQIAPALREKGLYFVGIDVI
                     GDYLTEINVTSPTCIREISTETGLDIAGEYMRCLEKLHTSLSK"
     gene            complement(6979..8259)
                     /gene="gshA"
                     /locus_tag="I9760_002406"
     CDS             complement(6979..8259)
                     /gene="gshA"
                     /locus_tag="I9760_002406"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAT5044165.1"
                     /translation="MPVPHLTTAHSGPLYHVEKIILNKLAEIESWFRKKWQETPAPLT
                     SSVDLRHAGFKLAPVDTNLFPAGFNNLNPEFLPLCIQAAQSALIEYIPDCTKILLLPE
                     SHTRNKYYLQSLNVLKTIFVKAGFIVRIGSLDPDIKEPTEIILEQGETLLIEPLQRQG
                     DKVGLDNFSPCLLLLNNDLSSGVPEILQDVQQRIRPTAKLGWASRLKSSHFQFFEEVA
                     IEFAELVGIDPWLINPYFSAIEGVDFMAQEGIETLAQEVDKILSLINDKYTTYGIENK
                     PFAVVKADNGTYGMSVMMVHNGDEIRQLNRKQRTRMSTSKGSRKVDRVIIQEGVYTFE
                     TMPDGSVAEPVVYMIGQFVVGGFYRVHKGRGIDENLNAPGMHFEPLAFAQACNMPCDD
                     LEVVDCPNRFYVYGVIARLAALAAAREIAAIGGE"
BASE COUNT         2511 a         1778 c         1460 g         2510 t
ORIGIN      
        1 agagaattaa ttattcagtc caataaatta ttggagtgtt gggtgacaga gttgattgag
       61 ctcaacgcta caacactaaa atactatccc agttgaggac aactgggaca tataaattta
      121 atccaaatat ttagataaat acttaattgc cagccgcctt tttaattaac tcacgtagtg
      181 attgctcgct tgtagcgcct ggtataaatg atatttcact gcccttttta tattgaccat
      241 ctggtgttga gccaattata aaggctggtg tacccatcaa atgtaatttt tcagccaatt
      301 ggcgattcgc atccaggata tcggtcactt cctgactatc catatctttt ttcagctttt
      361 gcatatccaa acctatggat tttgcggcat ccatcactgt tttttcatcc aaacgtttgt
      421 ctatcgtaat caaagcatta tgcatagcct gatatttgcc ttgcatgcca gccgccagag
      481 caactctgga agccagatca gaagtcttgc caaagattgg aaattctttg tatataactc
      541 gtaatccgct gtctttttta accagatttt caatggtaga tgccattttt ttgcaatgaa
      601 tgcattgata atcaaaaaac tcaaccagtg ttacattccc tttaggatta ccaacagtag
      661 ttaatttgcc ttgaaaaact tgttcagcat tttcctgaat tgcggcttga gcttgctgtt
      721 gcatattttg ttgctgtttt tgttgcaatg cttgcgaagc ctccaataaa acttccgggt
      781 tattaatcag ataatcatga atgacttttt caatttcctt tttttgagca tctgataaag
      841 aagcgctagc cgtatcagct gccattattg caggtgatac taatgtagag gctaatgctc
      901 cagctgttaa taaacttgta aatttcacgt aattcccctt acttataatt aatcaatgac
      961 gcaaattaca tctcgcagat aactttatca ttaattgatc tgctcaaaac agcaaagtat
     1021 aacaaatttc tgttgcctaa cgcaccctag cattcatgaa aaaaaaattc aatattacaa
     1081 tagcaaactg caagtcaggg tacctcagat attttataaa aattaaatgc aataactaca
     1141 ggcattcagt caaattagca taggccaaaa ccaaccattt tacaccatgt tcactgaatt
     1201 ttacttgaac tcttgtatga gccccgctgc cctcaatggc aagaacgaca ccctgtccaa
     1261 atttggcatg ctgcacattt tgcccaattg taatgcccga cacctcgtca gccactggca
     1321 atttgctttt tgtagttcca ggccactgag aacgtgattt aactcttact tcatcaagaa
     1381 attgttgagg aatttcacgc aaaaatcttg atgatctgtg atactcttca cgaccatatt
     1441 gtcttctgac ctcagcatag gataaaacca atttacgcat ggcacgagtc atgccgacat
     1501 aacataatcg acgctcttcc tccaagcgac caggctcttc aagcgattgt ctgcctggaa
     1561 atattccttc ttccatacca accaaaaaca caatcggaaa ctcaagccct ttggctgcat
     1621 gtaaggtcat caagtgaacg gagcgttcat gttcatcagc ctgcaattcc cccgattcta
     1681 atgaagcatg agcaaggaat gaattaacca acggtaactc ctcctcttca tcgtactcat
     1741 atcgaaattg tttggcagca ttaattaatt cttgcaggtt atccacacgt gattctgatt
     1801 tatcaccttt gattttgcta aagtgggcgt ataaaccact atgctgtatt acatcagaga
     1861 tctgctcatc caactcctta ttagcaacca caacctgtaa tttttcgatc aaatcaataa
     1921 acttggctaa tgccaagctc cccctttgcc ctaatcctgt ggattgaagt atgcccttgg
     1981 aagcgcccca caacgaacac tgttctgctc tggcgtactg gcgaacctca tccaatgttt
     2041 tttctcctat tcctcgagta ggaaaattaa ctactctttc aaacgcagta tcgtcatttg
     2101 gattaactaa caatcgtaag taagccaaag tatctttaat ttctgccctg tcaaagaaac
     2161 ggactcctcc atatatcctg taggctatac ctgcacgtaa caaagcctct tccaaaacac
     2221 gggattgggc attcgaacga tacaacactg caatctcatc cgcactggcg ccctgattta
     2281 attccatgcc aattcgttca gtaacaaaac gtgcctcatc caattcattg aatgcactgt
     2341 atacaagaat tttttctccc tcactaccag cggtccataa ctctttgccc atacgagtac
     2401 tgttattgag aatcaatgca tttgctgcat ttaatatcat tgcggtcgac ctgtagtttt
     2461 gttcaagcct tatgatatgt gtatctttaa aatcatgaac aaactgctga atattttcca
     2521 ctttggctcc acgccaacca taaattgact gatcatcatc tcctacagcc aataccgctg
     2581 tatgatcacc agcaagcaag cggatccatg catattgaat cgtattggta tcctgaaatt
     2641 catcaactaa aatagcctgg aagcgttcgc ggtaatgagc aagtatctct tcattatctc
     2701 gtagcaattc atgagttcgt agtaatagtt cagcgaaatc gattacccct gaggtttggc
     2761 aaacgtcttc atacgcttta taaattgcaa ccaaagtctt ggccggacca taatgcagtg
     2821 cattaatatg ttgtggacgc aagccttcat cctttttact attaataaat gcttgcgctt
     2881 gtttgacttg ccattgctca ggatcaagat taagtgaaag aatgactcgc ttgataactc
     2941 gtgcctggtc ttccgaatcc agaatatgaa actgttctgg aaggttagcc tccttgtagt
     3001 ggcggcgtaa taaacgatga cataatccat gaaaagtacc aacccataaa cccaacgtcg
     3061 gtgtagacaa catactgcta agtctcgatc tcatttcacc ggccgcttta ttcgtaaatg
     3121 ttactgctaa tatagcgtgc ggggagagat gctgttcttc aattaaccag gcaattcggc
     3181 tcaccaatac ttttgtttta ccactgcctg caccagctaa aaccagcata ttacctaatg
     3241 gggaggttac agcgtcacgt tgcttttcgt ttaatccctt gagaagtgct gctatggtca
     3301 tagaatattt ccaacagaaa aagtccaagt attatcttca atttaattgc taaagcaaag
     3361 atataatcga tgcggtaaaa aatctaaaaa ttctcctgga tatatatcga taaaacccat
     3421 acaagtattg ctttctgatc aatcagagcg caaaatactg ttcgtttaaa gtagctacta
     3481 gatagtatta taatacaatg acgttatcat aaaggactaa aatgcatacc ctctacccag
     3541 caattaaacc ttacacacaa cacgaattga aggttagtaa actgcataca ctttatttgg
     3601 aagaaaccgg aaatcctgaa ggcatacctg ttattgtatt gcaccatggc ccaggtggag
     3661 aagctgaccc gcatctaaga cgtttttttg atcctcaacg atatcgcatt attctttttg
     3721 atcagcgagg ttgtggccgt tcaattccac atttggaaat aagtgaaaat aatacccagc
     3781 ttctcatgga ggatatcgac tccattagag atttcctggg cttaagggaa tttgtgttat
     3841 ttgggggtgg atggggttct ctgttagcat tattgtatgc gcaaaaattt cctcaacagg
     3901 tgaaagcatt gctactccac caaatttttt taggtcgaca aaaagacatc gattggtttt
     3961 ataagcatgg agccagtctg gtttatcctg attactggga agagtttacg aacacagtcc
     4021 ccgaaaacaa acttgataat atcccccaat attatgcgga atgtctacaa ggctctaatg
     4081 agctagcgcg aatggctgct gcgaaaaact gggctctttg gcaagccaga tgcagtagtt
     4141 tacagccaca cttatatgtt attgatcagt atagcgaccc acatttcgca ttagcattag
     4201 caactctgga atcctattac attattaatc attattttat tgaagaaaat caggttatag
     4261 ctaatgccca taaaataagg cacattccta cctatattgt tcatggccgt tttgacttgg
     4321 taagcccttt atccagcgca tgggaacttc atcaagcgat ccccgcttcc aatctaagaa
     4381 ttgtcagaga tgccggtcac tctgatagag aatcgggaat tattgatgca cttatttatg
     4441 ccagtaagga catctcaaag cagggtcttg atgcatgtta actgtattac aaagagtaaa
     4501 ggaagccagg gttgatattg atggtcaaac agttggcaaa ataaatcatg gcttgctgat
     4561 actttgtggt tttgaaccta aggattcgct agaaaatatt aaacgaatgc tggataaatg
     4621 cattaattat cgcatttttg aagacccgag tggcaaaatg aacttgagtt taaaggatgt
     4681 aaatggcggc ttgctattag taccacaatt tactctcatg gcagataccc aaaaaggatt
     4741 gcgtccaagt ttttcaaatg cagcttctcc agaactcggg cgtgagcttt ttgataactt
     4801 attaactctt gctcagaaat gccaccaaaa tactcaaagt ggttgttttg gagcgaacat
     4861 gcaagtttat ttatgcaatg atgggcctgt gacatttctg ttacaatttt aattagtttt
     4921 tactgtatta aatttttgta taacctgaat tgtcgagcaa aatacaatca acattcatta
     4981 ttggaattca ttccggccaa aaaaaagcat gtttgtttca gttaaaaagc agcaatttaa
     5041 aaggcctatt ctttacatca aagcatttgt ttgcaagaac caaaaactgg tgtaacatca
     5101 ttttaagtat ttaataaata gaataattat gcgactaatc ttaatgtttg ctagcatcat
     5161 gggatccgtt attttagcga gctgtgcaag taaaggatca aacccgatcg atccattcga
     5221 accctttaat cgtaaagtgc acaattttaa tatggcattt gatgctaccg tattaagacc
     5281 accagcaaaa ttatatcagg ctgtcgtacc aaaattggta agaaaaggtg tgaataatgc
     5341 attcaataat ctggatttgt tccctacaat agccagcgat atactgcaag ctgaaggaaa
     5401 atgggtaatc aaagacagtt ggcgactctt cattaactcc actattgggg ttgctggtct
     5461 gtttgatgta gcagaaaaat ttggtttgcc accgcattac aatgatctgg gcctaacact
     5521 cgccaaatgg ggcgataaaa aatcgcctta ccttgtaata ccctttttag gccccagtac
     5581 tcttcgtgat ggagccggct ggctgttcca gtttgcactc tggagtcctt atgtatatat
     5641 tgatgatgct ggtctggttt atggtttaat tggattacgc tatattgatt tacgaacaca
     5701 atttttcgac tctgagcctt tactgaatga agcattagac aaatacacat tcatcagaga
     5761 cgcttacctg caacatagaa attatcttat tgcagggact gagcaagata caggcacgtc
     5821 cttcgttgag gataagaaag ctaacaaaac tgcaatgcaa gaatcaaccg cttcaaataa
     5881 ccaactaggt tctgactatg ttgatgaata aaatgattaa ttgagacttg ccaggtcatg
     5941 ataagggttg gtattgagcc aaatgcaaaa ccaaccctct ttaagctctg aacttcaaaa
     6001 tcaaaataat gactgttctt tattttgata aagaagtatg taacttttca aggcagcgca
     6061 tatattcacc tgcgatatcc aaaccagttt cagtgctaat ttcccgaata caagtgggac
     6121 tagtcacatt aatctctgtt agataatcac ctatcacgtc gatacccaca aaatacagac
     6181 ctttttctct caaagctgga gctatttgtt cacatatcca tttgtctctt tcagtaatag
     6241 ctaccacttc tcccctggct cctgcggcca aatttccccg caaatctcct tttgcaggaa
     6301 tgcgagccaa agcaaaagga acgggttcgc cattgatcaa taaaatacgt ttgtctccgc
     6361 ttactgtaat atccgggata taacgctgag ccataataca ttttgtctgc cctttggtta
     6421 atacctccaa aatgaccgat aaatttttac cttctttgtc aacataaaaa acggaattac
     6481 ctcccattcc ttcaagaggt ttaaaaataa cttgttgatg aatttgccag aactctttta
     6541 attgccgagc atctctggat acaaaagtag ggggacagca atgaggaaaa tttagggtaa
     6601 aaaatttctc attcgcgtca cgtaaacttt gcggtttatt tgcaaccaac accccttctt
     6661 gttccgctaa atccaaagcg taagttgcat aaatatactc catatcaaat ggaggatcct
     6721 tacgcatcaa aataacatca aaatctctca atggcttatt acccaaatct atggttttaa
     6781 cccaactcag actctctata tctccaatgt gaatttcatg gactcgtgca taagcgcccc
     6841 catcacaaca atataagtca ttcagggtaa agtaggcgca agaccatccc aatccttgtg
     6901 cgcttttaat catagctact gtagagtctt tatagggttt tatttgatcc aatggatcca
     6961 taagcaccgc aagtttcatc attcgcctcc aattgcagca atttctcgag ctgcagccag
     7021 tgcagccaaa cgagctataa ccccataaac ataaaaacga tttgggcagt caactacctc
     7081 taaatcatca catggcatat tacaagcctg tgcgaaagct aacggctcaa aatgcatgcc
     7141 tggagcattg aggttctcat caattcctct gcctttatga acgcgataaa atccaccaac
     7201 aacaaattga ccaatcatat aaactacagg ttcagcaaca gaaccatcag gcatcgtttc
     7261 aaaagtataa acaccttcct gaataatgac tctgtctacc ttcctgcttc ctttactggt
     7321 agacatcctg gtacgctgct ttctattcaa ttgtcgaatt tcatctccat tatgaaccat
     7381 cataacgctc atgccataag taccattatc tgcctttact actgcgaacg gtttattttc
     7441 tattccgtaa gtagtgtatt tatcgtttat taacgataat atcttatcaa cttcttgagc
     7501 taaggtttca atcccttcct gggccataaa atcgaccccc tctatagcgc taaaataggg
     7561 attaattaac cacggatcaa taccgactaa ttcagcaaac tcaatagcta cttcttcaaa
     7621 aaattgaaaa tgactcgatt ttaatcgaga agcccatcct aatttggctg taggtctaat
     7681 tcgctgttgc acatcctgta atatttcagg aacgcctgaa gacaaatcgt tattcaataa
     7741 caaaaggcaa gggctgaaat tatccagtcc caccttatca ccttgtcttt gtaatggttc
     7801 aattaataaa gtttcacctt gttcgagtat tatttctgta ggttctttaa tgtctggatc
     7861 taaactgcct attcgaacta taaaaccggc ttttacaaaa attgttttta atacattcaa
     7921 actctgtaaa taatacttat tccgagtatg gctttctggg agaagcaaaa ttttagtaca
     7981 atcagggata tactctatca aggcagattg agcagcttgt atacacaggg gaaggaactc
     8041 cgggtttaaa ttattaaaac cagctggaaa taaattggta tcgacgggag ccaatttaaa
     8101 gccagcatgt cgcaaatcaa cagatgacgt tagaggtgcc ggtgtttcct gccacttttt
     8161 tctgaaccag gattctattt cagcaagttt atttaatatt attttttcca catgatacaa
     8221 aggacctgaa tgagcagtag tcagatgtgg aacggggat
//
LOCUS       DACTJE010000147         8252 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.147, whole genome shotgun sequence.
ACCESSION   DACTJE010000147 DACTJE010000000
VERSION     DACTJE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.147"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            96..818
                     /locus_tag="I9760_002407"
     CDS             96..818
                     /locus_tag="I9760_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HAT5044166.1"
                     /translation="MNYQYSPNLKKKFLLALTTLFIVQMFYFATVRASTRDTQLWTLM
                     NAIGPINKQHRKIRYWLEIQDRVGENISQLSQLILRPGIGYELLPTTTIWLGYDRIYT
                     AQPFANPPVNENRVWQQLLWSKEYTRLRLTARSRLEERFIQRATHTAWRYRQLFKTSI
                     FIPKHPKYAAVISDEAFFHLNDFNLQRNQGFDQNRLFVGLGYRTSKNSTIEIGYLNQM
                     IKRVNNPDYRGDYLSMALLLTE"
     gene            complement(877..1233)
                     /locus_tag="I9760_002408"
     CDS             complement(877..1233)
                     /locus_tag="I9760_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044167.1"
                     /translation="MSKFFTLLICISIGLLSFPTYAQKTIRLSPNETKTLKNSTLWTL
                     NATCNVQSSTKVNGKIKIIVLKNNGKVNGKSLSTGQGTFITIKNNSSISVSAESGTQI
                     NLINLGNQELQAVCST"
     gene            complement(1361..2122)
                     /locus_tag="I9760_002409"
     CDS             complement(1361..2122)
                     /locus_tag="I9760_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044168.1"
                     /translation="MKLHSISTNKMRLIISMWLIYLFAVLFLVASLFTMLILMMEYTL
                     VCKNKQYHLAKECVLHSRIYNLYHSSTELGDLKAAVVKSSRSSKGNTLYYVDLLTAQG
                     PVNLTGSSSSGRDDKEIAANSINNYISNSLETTFKVSYPASWWMYGLSVFFLLVGALL
                     LTVRGAITDFDRILKTIVIKRKGLFKTEETKLSFSDVDKIIVEESLGSRGTKTYRLAI
                     ALKDKPPIPLVTTYDSSFAKKEKIANQLNQFIHEN"
     gene            2386..3411
                     /locus_tag="I9760_002410"
     CDS             2386..3411
                     /locus_tag="I9760_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044169.1"
                     /translation="MGWTDRFWNAYRVVTNTVIEGAANSLNAAGSLACLLGGAGVSLS
                     YSMSETIKAGYFGSVGVAGTVNLDVTAVEFNYGFNQTIPFRRDLLEKSDGGSYALNDF
                     ANPGTIFMISSLAILSGTTLRTFGANLKKWQQNREDKHHFITKYGTDLEPPSYKEYLH
                     VNAESFFNSLAIASFSSLLAKNVLDFSIKDLSKYHFTYPFNGTNITGSVKTPDYTGPV
                     TSEFFPLDFFVSQNTTIHTDYLNLIVNEKVKANAMTNVTYGGGVHLEPNHQSSAPLLA
                     ASATIGISTYLAGTFFGKKILRERDERLQHSRSSSYSIVSGTFWDNKHDIEAKGDTKF
                     EHSHSLQ"
     gene            3474..4667
                     /locus_tag="I9760_002411"
     CDS             3474..4667
                     /locus_tag="I9760_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAT5044170.1"
                     /translation="MVFKSRLVGIILLCFSSITGWCLSQSPEEDRIIVSNCLYEQLKD
                     KARLIAQSDHFSFIAIPIDENITNTLQKMRPVCGNYLNAEPYIQQTSNRFLDSKKLLD
                     KLTSYHSISTANPYPINHEAQVHSLFEQIDPAKIWQTNQHLTSYINRSAKSRTGVEAA
                     QWFKQQFDTLAQDYGRKDVESYFVKTGNKFIQPSVVTVIGKDKPGEAIVIGAHIDTLD
                     GNMPGADDDSSGISVELEMARVVFSSNFELNRPIYFIAYAAEERGLIGSGYVVQDFLQ
                     KKIPVKAVMQLDQAGYRANAKDQTIWLLKDYVDKGLTEFTAELLTRYVKTPVGYTKCG
                     YACSDHVNWTNEGFKTTYPSATTLDDDNPYVHTSNDTLDILNLEHMVNFTKLGLAFIV
                     ELGLN"
     gene            4726..5616
                     /locus_tag="I9760_002412"
     CDS             4726..5616
                     /locus_tag="I9760_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044171.1"
                     /translation="MKTEALLSWLNVESNESYDSIRIKYQLRLEALEKEERIKKMGGI
                     NPFAPKIPLNNPRIITSKEIQEAWAAIDSLSKYQQFKGLVAQEENLSTIPNKKMDSKR
                     EETKLTHFVRERAQGPRGRRLPAHLQAKIKRVSLDKPHSKLITEGAEQQINAILDKTT
                     LSVSDLQSAVYTAVQNYTNYHMKSENKNNPKYNRGQGDGFFSFFRHGSFGITTANNLY
                     TSINAKNATLDETIEKLKDFLGHSSRAYHHHSFTSYLADALAEKGLLTSHPASRYKQE
                     DVVNQLDQWIAQNSTVNFLK"
     gene            complement(5728..>8252)
                     /locus_tag="I9760_002413"
     CDS             complement(5728..>8252)
                     /locus_tag="I9760_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5044172.1"
                     /translation="NLFCISFWFDSIDCNFEMLMPFFKDILGLLNLSLAKLMNKKREK
                     LLTTILEKAGDSVEITNEEAVIQYVNPAFENITLYNAREAMNKTVASLLRSPVEDAQL
                     FEEINQTLKSGNTWKGQIRSRKKDGSYWVAQTIIVPVMDDELGRITQHIAIKQDITEQ
                     VHRFNQLKISEERYRNLMNAVADGIFIHNLQGAFLEVNEAACLSLNYSREELINSYVW
                     DIEVGFSQESLNQLWENVQKGPINTEGMHRRKDGTTFPVDVRLAVFNATGEKLVLAIV
                     RDITLQKRSEATIRKLTRALEQSPVLVIITDKTGTIEYVNAKVIEQTGYRKEELVGGN
                     TRILRSGQTPHEIYKTMWEHLANGEEWHGELLNKNKSGEHFWVSAIISPIRNDNEEIT
                     HYLAVMEDVTQKKSYEEMLKHQATFDNLTNLPNRLYAVNKLERAISKSHINNQKLAVL
                     FLDLDEFKQINDSLGHAIGDLLLKALSERFLSMIRQTDTIARLGGDEFMMILENIMQD
                     KDAESIAKKCREICSQPFVIESQELLVSTSIGIAIYPEHGKDAKTLMRNADTAMYQSK
                     IRGKNNWTVFQNTMIEGANNHIRIKTELHQAFTRNELYICYQPIIELKENKVVAAEAL
                     LRWQNTTLGQINPDQIIPLAEETGMIIPLGYWILDKVCTQAKEWEFITGSKIKVAVNI
                     STLQLKQKDFIHKVQLILKRTKLSADSLIFEVTESAFIDDSKFILSQLNKLSDMGIHF
                     SLDDFGVGYSFLQYIKSYPFKNLKIDRVFIQGVNTNHNELSLINSIITMARNLKLSVI
                     AEGIETAEQLELMRSMNCDMVQGWYFSKPLSGDKFLFYLMNK"
BASE COUNT         2639 a         1652 c         1400 g         2561 t
ORIGIN      
        1 agctatttta taaaaaaatt gaacaataaa ataaaaaata ttgacgatgg tttctcaaac
       61 ctgatgtgca gtaacttttt aaatcttttt aaataatgaa ttatcaatac tctcccaacc
      121 taaagaaaaa attcctgctc gcattgacta ctttatttat tgtacaaatg ttctattttg
      181 ccactgttag agcatcaaca agggatactc aattatggac acttatgaat gcgataggac
      241 caatcaataa gcaacatcgc aaaataagat attggcttga gatacaagat agagttggtg
      301 aaaatatttc gcaattgtcc caactaatat tacgccctgg cataggatat gaactcttgc
      361 ctacaacaac tatatggctc ggatatgata gaatttacac tgcgcaacca tttgccaacc
      421 ctcctgtaaa cgaaaatcga gtttggcaac aattattgtg gagcaaggaa tatactcgct
      481 taagacttac tgctcgttcg cgtttggaag agcgctttat ccaaagagct actcataccg
      541 cctggcggta tagacaatta tttaaaacca gtatttttat tcccaaacac cctaaatacg
      601 cagccgtgat tagtgatgaa gctttttttc atctgaatga cttcaatctt caacgtaatc
      661 aaggctttga tcaaaaccgt ttatttgttg gcttaggtta tagaaccagt aaaaacagca
      721 ctatagagat tgggtatctc aatcaaatga ttaaacgcgt aaataaccct gactatcgtg
      781 gagattattt atccatggct ctattattaa cagaataaac aagcgtacaa aacaaaccat
      841 tactttcaat agtttgtttg agaaaaattg atttaattaa gtagaacaaa ctgcttgcaa
      901 ttcttgatta cccaagttaa tcagattaat ttgagtccct gattctgcac taaccgagat
      961 actgctgtta ttttttattg taataaaagt tccctggccg gttgaaaggc ttttaccatt
     1021 tactttgcca ttgttcttta aaacaatgat ttttatttta ccattaacct tcgtgctgct
     1081 ctgtacgtta caggtcgcat tcaaagtcca taaagtgcta ttttttagcg tcttggtttc
     1141 gttagggcta agtcgtatcg ttttttgagc ataagttggg aagcttaata aaccaattga
     1201 gatacatatc aacaaagtaa aaaatttaga catgctactt tccttctcca gaatctaaag
     1261 cccaactatt ttaacgcaat atgtatacat tatatacaga ttcacttaag attttattaa
     1321 gcccgtttat aaattttttt tgaagtgccc cattcagcaa tcagttctca tggataaatt
     1381 gatttaattg gtttgcaatt ttttcttttt tagcaaaaga agaatcatac gttgttacca
     1441 aaggaatagg tggcttatct tttagagcga tggcaagtct ataagttttc gtacctctgc
     1501 tcccaagtga ctcttcgact attattttat ctacatcaga aaacgataat ttggtttcct
     1561 ctgtcttaaa caatcccttt cttttgatta caattgtttt taatattctg tcaaaatcag
     1621 taattgctcc tctaacggta agcaacagag cacccaccaa tagaaaaaaa acggataagc
     1681 catacatcca ccacgaggct ggataactca ctttaaaagt tgtttccaag ctattactta
     1741 tgtagttatt gatgctgttg gcagcaattt ctttatcgtc acgcccgctg gaactgctcc
     1801 ccgtcagatt gactggacct tgagccgtta acaaatccac ataataaagc gtatttcctt
     1861 tacttgagcg actgctttta actacagctg cttttaaatc acctaactcg gtgctggagt
     1921 gatataaatt ataaattctg ctgtgtaaaa cacattcctt agccaaatgg tattgtttgt
     1981 ttttgcaaac gagggtgtat tccatcatta aaataagcat tgtaaaaaga cttgctacaa
     2041 gaaacaaaac cgcgaacaga tatataagcc acatgctgat gatgaggcgc atcttgtttg
     2101 tgctgataga gtgtagtttc atttcatatt attgctaata acttatttta aatatagcaa
     2161 caactttcga ttatatgtaa tagttaatac cattagtcaa cgtgaaaacc attccataat
     2221 gacctaaaca aaaccacaaa ggggctattt ttctactaaa tgctattttt tgccacaaat
     2281 aaatctggct tttaaaaaaa atatgttatt ctttccaaca tcactgattc gtacttaatc
     2341 aaagtggcga atttattttt tatcatttaa caaaagactt taaatatggg atggactgat
     2401 cgattttgga atgcgtatcg cgtggttact aataccgtaa tcgaaggcgc ggcaaactct
     2461 ttaaatgcag ctggatcatt agcttgcctg ctcggaggcg ctggggtctc tctctcttac
     2521 tccatgtctg aaacaataaa agcagggtac ttcggatcag ttggggtagc aggcacagtt
     2581 aatttggatg tgactgcagt agaatttaat tatggattta atcaaaccat tccttttcgc
     2641 agagatttac tggaaaaatc cgacggtggc agttatgcat tgaatgattt tgccaatcca
     2701 ggtactattt ttatgatcag ttcgctggct attctttccg gaaccacttt aagaactttt
     2761 ggggctaatc ttaaaaaatg gcaacaaaac agagaagaca aacaccactt tattaccaag
     2821 tacggcactg acctggaacc cccttcttac aaagaatatc tgcatgtcaa cgcagaatcc
     2881 ttcttcaatt ctttggcaat agcctcattc agcagtcttt tggcaaaaaa tgttctggat
     2941 ttttcaatta aggatttatc gaaatatcat ttcacttatc cttttaatgg aacaaatata
     3001 actggctcag tgaaaacgcc tgattatact gggccagtta catcagaatt tttccccctt
     3061 gacttttttg tgtctcaaaa tacaaccatc cacacagatt atttgaacct gatagtcaat
     3121 gaaaaagtga aggccaatgc aatgaccaat gtcacttatg gtgggggagt gcatttagag
     3181 cccaaccacc aatcaagcgc cccccttctg gctgcttctg caactattgg tatcagtacc
     3241 tatttggcag gcactttttt tggcaagaaa atcctgcgag aacgtgatga aagacttcaa
     3301 cattcaagat catcgagcta ttccatagtt tcaggcactt tttgggataa taaacatgac
     3361 atagaggcaa aaggcgatac aaaattcgaa catagccatt cattacagta aagaatagcc
     3421 taagtctcct ccatgtaata tattgcaatg aaaaaaataa aacggagttt ttcatggtat
     3481 ttaaatcaag gttagttggc attattttat tgtgcttttc aagtattact ggatggtgtc
     3541 tttctcagtc ccctgaagaa gatagaatca ttgtttcaaa ctgcctgtat gagcaattaa
     3601 aagacaaagc caggttaatt gcgcaatcgg atcactttag cttcattgcc attccaattg
     3661 atgaaaatat taccaatact ctacaaaaaa tgagaccagt ttgtggaaat taccttaatg
     3721 ctgagccata tatccaacaa acctccaatc gttttctcga ttcgaaaaaa ttattggata
     3781 aactgaccag ctaccactcc atctcaaccg caaacccata cccgattaat catgaagcac
     3841 aagtccatag tttgttcgaa caaattgacc cggcaaaaat atggcaaacc aatcagcatt
     3901 taaccagtta tatcaaccgc tctgccaaat ccagaacggg agtagaggct gcacaatggt
     3961 ttaaacagca atttgatacc ttagctcaag attatggaag aaaagatgta gagagctact
     4021 tcgtcaaaac cggaaataaa tttatacaac cctcagtcgt cactgtaatt ggaaaagata
     4081 aaccgggaga ggccattgtt ataggggcac atattgacac actggacggc aatatgcctg
     4141 gtgccgatga tgactctagt ggaatatccg ttgaacttga aatggcaaga gtggtatttt
     4201 catcgaactt tgagctcaat agaccaattt attttatagc ttatgctgct gaagaaagag
     4261 gacttatcgg ctctggatat gtggtacaag attttttaca gaaaaaaatt ccggtgaaag
     4321 cagtaatgca gttagaccag gcaggataca gggctaacgc caaggaccaa acgatttggc
     4381 tgttaaaaga ttatgtagat aagggtttaa ctgaatttac agcagaatta ttaactcgct
     4441 atgtcaaaac tcctgttggc tatactaagt gcggttatgc atgcagcgat catgtgaatt
     4501 ggaccaatga aggctttaaa accacctatc cgtctgcaac tactttagac gatgataatc
     4561 cttatgttca tacctccaat gatactttgg atattctgaa tctggaacat atggttaatt
     4621 tcactaaatt aggcctggcc tttattgtag agcttggttt aaattagcga tgaaagcaat
     4681 agagctcgtg ccaatttaac ggtgctctgt aatgggagtt tattaatgaa aacagaagcc
     4741 ttacttagct ggctaaatgt agaaagcaat gaatcctatg attctatcag aataaagtat
     4801 caactaagac ttgaagcctt agagaaagaa gaacgtatta aaaaaatggg aggtatcaat
     4861 ccttttgcgc ctaaaatacc ccttaacaac cccaggataa tcacctccaa agaaatacaa
     4921 gaggcatggg ctgctattga ttctctcagc aagtaccaac aattcaaagg actagtggct
     4981 caagaagaaa atttatccac tatccctaac aaaaaaatgg atagcaagag agaggaaaca
     5041 aaattaaccc actttgtaag agaacgggca caaggccctc ggggaagacg cttacccgcc
     5101 catttacaag ctaaaattaa aagagtctct ttggacaaac cacacagtaa gctaattact
     5161 gaaggggccg aacaacaaat taatgcaatt ttagacaaaa ccaccttgtc agtgagtgac
     5221 ttgcaatctg ctgtttacac cgcagtgcag aattatacga attatcacat gaaatccgaa
     5281 aataaaaata atcccaaata caacagagga caaggtgatg gttttttcag tttttttcgc
     5341 catggaagct ttggaatcac tacagcgaat aacctatata cttccatcaa cgcaaaaaat
     5401 gctactttag atgaaaccat agaaaaatta aaagattttt taggccattc ctccagagca
     5461 tatcatcatc attctttcac atcttacctg gctgatgcac ttgctgagaa gggactatta
     5521 acatcccatc ctgccagccg gtataaacaa gaagacgtag tgaatcagct tgaccaatgg
     5581 atagcacaaa actcaactgt taatttttta aaatgataat ggcaagggta gtctgttttt
     5641 tactcctgaa gtaaaaacaa ataagttaga atccgccacc gacatccgtg ccaaatcttt
     5701 aaattaggca cggcagctgg tacattttta cttattcatt aaataaaaca aaaatttatc
     5761 cccactcaaa ggcttggaaa aataccatcc ttgtaccata tcgcagttca ttgagcgcat
     5821 taactccagc tgttctgctg tttctattcc ttcagcaata accgataatt tcagattgcg
     5881 cgccatagtt ataatactat taatcaaact taactcatta tgatttgtat ttacaccctg
     5941 gataaaaacc cgatcaattt ttaaattttt gaacggatag gacttaatat actgcaggaa
     6001 agaataaccc accccaaaat catccaaaga aaaatgaatg cccatgtcac ttaatttgtt
     6061 taattgagat aaaatgaatt ttgaatcatc aataaatgcc gattctgtaa cttcaaaaat
     6121 tagagaatct gcggataatt ttgttctttt caaaatgagt tgcaccttat gaataaaatc
     6181 cttttgcttt aattgtaagg tagagatatt caccgctact tttatcttgc tgccggtaat
     6241 aaattcccac tctttcgctt gtgtgcacac cttatccaaa atccaataac ccaatggaat
     6301 aatcataccg gtctcctcgg ccaagggaat aatctgatca ggattaattt gacctaaggt
     6361 tgtattctgc caacgtagca aggcctccgc agcgacgacc ttattttcct tcagctcaat
     6421 gatcggttgg tagcaaatat ataactcatt acgggtaaac gcctgatgta attccgtctt
     6481 aatacggata tggttatttg ccccctcaat catggtattt tgaaataccg tccaattatt
     6541 tttgccccgt atcttgctct gatacatggc tgtatctgca ttacgcatta acgttttggc
     6601 gtccttgcca tgctcaggat atattgcaat tcctatactt gtagatacca atagctcttg
     6661 tgattcaata acaaaaggct gagaacaaat ttcccggcat ttttttgcga tactttcagc
     6721 atctttatcc tgcatgatgt tttctaaaat catcataaat tcatcaccac ctaagcgtgc
     6781 aatagtatcg gtttgccgta tcatagatag aaagcgctcc gataacgctt ttaataataa
     6841 atctccgatg gcatgcccta aagaatcatt aatctgttta aattcatcta aatctaaaaa
     6901 cagcaccgcc aatttttgat tgttgatgtg ggattttgaa atagctcgtt ccaatttatt
     6961 aactgcatag agacgattgg gaaggttagt taaattatca aatgttgcct ggtgttttaa
     7021 catttcctcg tagcttttct tctgagtcac atcctccata acagccagat aatgagttat
     7081 ctcttcatta tcattacgaa ttggagaaat aattgctgat acccaaaaat gctctccgga
     7141 tttgttttta ttcagcaact caccatgcca ctcttcccca ttggctaagt gttcccacat
     7201 ggttttataa atttcatgag gagtttggcc tgaccgtaaa attcttgtat ttccaccaac
     7261 caattcttct ttgcgataac ctgtttgctc tatgactttt gcattgacat actcaatggt
     7321 tcccgtttta tcagttatta tgactaaaac agggctttgc tctaacgctc gtgttaattt
     7381 tcttatagtt gcttcagagc gtttttgaag tgtaatatcc cgtacgatag ccaaaaccaa
     7441 tttctcccca gtcgcattaa aaactgccaa acgaacgtca acaggaaaag ttgtgccatc
     7501 ttttctgcgg tgcatgcctt ctgtattaat ggggcccttt tgcacatttt cccacaactg
     7561 atttaaactt tcctgagaaa atcccacttc aatatcccaa acataggaat taattaattc
     7621 ttcccgacta taattcaaag acaaacaagc tgcttcatta acttctaaaa atgctccctg
     7681 caaattatgg ataaaaatac catccgctac agcattcatc agatttcgat atcgttcttc
     7741 actgattttt agttgattga atcggtgtac ttgttccgta atgtcttgtt taattgctat
     7801 atgttgggta attcgcccca attcatcatc catcacagga acgatgatgg tttgagcgac
     7861 ccaatagctg ccatcctttt ttcttgagcg tatttgcccc ttccatgtat tccctgattt
     7921 aagggtttgg tttatttctt caaacaactg agcgtcttcg accggtgagc gcaataagga
     7981 cgccactgtt ttattcattg cttcacgggc attatacaaa gtgatgttct caaaagcggg
     8041 attaacatat tgaatgacag cctcttcatt cgttatttcg accgagtcgc cagctttttc
     8101 caaaatagtg gtcagcagct tttccctttt tttattcatc aatttggcta aagacaggtt
     8161 taataaaccc agaatatctt taaaaaaagg cattagcatt tcaaagttac aatcaataga
     8221 atcaaaccaa aaactaatac aaaataaatt at
//
LOCUS       DACTJE010000148         8092 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.148, whole genome shotgun sequence.
ACCESSION   DACTJE010000148 DACTJE010000000
VERSION     DACTJE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.148"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            130..1023
                     /locus_tag="I9760_002414"
     CDS             130..1023
                     /locus_tag="I9760_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044173.1"
                     /translation="MFSLKKTTAAVFALGSSALFAGTMGPVCTPGNVTVPCERTAWDI
                     GITALYLQPTYDADWGYNGFTDVGGWRNWHDVDLEWDWGFKLEGSYHFNTGNDINVNW
                     YHLDANTDHWAFVDQLHAYDITWDAVNAELGQFVDFSANKKMRFHGGVQYAYIKTDVN
                     RHLNGFFLDNFNSKFNGFGPRTGLDMNYVFGNGFGIYAKSAVAILVGTSKFVDNCTVC
                     GFSYGSKNAIVPEVEMKLGADYTYAMAQGDLTLDVGYMWFNYFNALHNTAAVNVGLGT
                     SLETDFSASGPYIGLKYVGNV"
     gene            1344..1994
                     /locus_tag="I9760_002415"
     CDS             1344..1994
                     /locus_tag="I9760_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1975 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044174.1"
                     /translation="MGYNETIYHKPFIKINMPDVLGKPDATQIPDDVWLDILINYLQP
                     QDIVNLSEINNRLSRLFKTQHTQSEQLNQKKTLGILKVFAGYSSTFLFARLNGKQRLF
                     KTRNWSAADSDNINPILMPISLPENISVIESIQSTYKHTVVVGRDKHDQIIVATNKIN
                     QDYAKQETSHNQLIPLPLRDINISLVSAGNEEDYILNPIFSFPATQQGNCILNCIK"
     gene            1970..2830
                     /gene="pieG"
                     /locus_tag="I9760_002416"
     CDS             1970..2830
                     /gene="pieG"
                     /locus_tag="I9760_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PieG/LegG1"
                     /protein_id="HAT5044175.1"
                     /translation="MHLELHQINGNDPLGTIIGERNYQLPIPLPYTMKTVKQIHSGIW
                     HTVIRGYDQYNRPQIATFGKNYDGQLGTGDCENRDTPTPIKLPPEMLTFHAIQTGFFH
                     TIATGLDKNGFPIVASCGYNSDGQLGCGDKDNKNTLIPIKLPENMKSVEMVATGVKHT
                     VIVGKDINNKPVVATCGHNGYGQLGTGDEENRLCLTPIGIPEDITSVDYVGAGAYHTV
                     ICGRNIKNQPIITLCGCNSDGELGFAPQKIAKKSSLMTNPAMFFPPRQDKSAINNLMK
                     ISKFSPCNLL"
     gene            3020..3604
                     /locus_tag="I9760_002417"
     CDS             3020..3604
                     /locus_tag="I9760_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HAT5044176.1"
                     /translation="MPTSKKILMIVGDFNEDLEVYFPYQAMLMNNYLIDTVSPGKNKG
                     DFIQTAVHDLDKPHLQSYTEKLGHLFPITADFSKITLKDYDALIIPGGRAAEYQRLNK
                     DILAIIRHFHDADKPIACICHGIQILAEAGILEDKKCTTVGFCEPDVRKAGGHFIDTG
                     MDGVVVDGKLVTGATWLGNAPWMRAFLHILQNEG"
     gene            complement(3675..5609)
                     /gene="setA"
                     /locus_tag="I9760_002418"
     CDS             complement(3675..5609)
                     /gene="setA"
                     /locus_tag="I9760_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SetA"
                     /protein_id="HAT5044177.1"
                     /translation="MYKIYSYLGWRIDMKTENLPQAGQEAQIDKKIHFIWVGHIMPQK
                     NIQVVSEWAEKNPGYETIIWVDKKIAPAKELDLFILDMKSKGITVKDINEEGVCRDSI
                     RHELDQESPNYGMVSDMLRLNILAAEGGIYLDSDILCSAPFPDEIYAPFGFLLSPWSQ
                     GANNTLCNDIILCSKGNQIIQQLADAIEQSYIARDSFEFTHEYASMKETKGERIAKTL
                     GVTGPGFLFHQLKKMGILNDKSEMEAIHWELQDQRYLIDGSVKEPDYFYVPQNNTNDA
                     SWVPSIKRPGIENMSFQERLENAVQLIAFDIQKTGLFNLDHYANELKVKQNSWCIAAE
                     TSPELKPDSYLLIRPRDKTGEWTLYYVDEDKKLNPVTLPVIKGAIKLSEVSDPLRKFH
                     TLLSQVSDPVNPTAHELKQIGRALIELKPRQDEWHCKNKWSGAEEIAQELWQRITSNE
                     TLRAQIKQCFTQFESLKPRVAELGLTRASGAGTEVEAHESTVKEQEIISQNTVGEEGT
                     KEKNSVQLASENSSDEKIKTAHDLIDEIIQDVIQLDGKLGLLGGNTRQLEDGRVINIP
                     NGAAMIFDDYKKYKQGELTAESALESMIKIAKLSNQLNRHTFFNQRQPETGQFYKKVA
                     AIDLQTTIAAEYDNNHGLRI"
     gene            5845..7212
                     /locus_tag="I9760_002419"
     CDS             5845..7212
                     /locus_tag="I9760_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044178.1"
                     /translation="MKTLTTRIFSLIMLLALHFNPIVFAHTNNTNNKGLSASINNGVG
                     SSSSNNTYVTPQAFWNLYFDFTGDETPGYPKGKINISQTLFQSEMKKNSSLAQQNEGQ
                     LILFINSTLYIYNSDRQLKLKQLMRTAPNSGFTEMTAISHIGPALMYLAKIKENGDAS
                     WKSQMENLLKDIQAVKVINAQTPNNWLEQVNAPAWKPHLTTIHNMIDYACSMAGNYMS
                     DVLNEKLSFDMASLQNDFLNGNKTYPIPYNNVMIGTFMLTALQSMDQLHSKISQLKID
                     WPHAKVIIRFVAGSNVSAGVSKGSNWLVPFVQALSNNKLATDRIYITPYAAVKPSLGA
                     QELTQADYNYYNNTVWGARHNRRIIANEVFTNITSIFLPDRPAIPGDYTYSKPPKIED
                     FLMRLKFSLAEPTEMLSNTVGFWMAGELAEKNWNYNKISIPGITTGFPEGISTYPNNN
                     PVIQR"
     gene            7209..7511
                     /locus_tag="I9760_002420"
     CDS             7209..7511
                     /locus_tag="I9760_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044179.1"
                     /translation="MIKKITRKYYFIAYISYFRTIQNGTCPLSIFKNIGSKRYNTFSL
                     DSAQYLAIPQLSKDEPGIPANINGGNAKVDVLIFKWINGKFHLYQKIPGHGNEGIE"
     gene            7685..7981
                     /locus_tag="I9760_002421"
     CDS             7685..7981
                     /locus_tag="I9760_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044180.1"
                     /translation="MPSGKGIPSEAMYIRDFEKSLYEYCESRGVQFKKGTFSGLSDNL
                     VSYKDTKENIRTVKCDMVVDCSGSNRMVLKETTHPRRNRSLAPGETTMQITTKI"
BASE COUNT         2538 a         1639 c         1470 g         2445 t
ORIGIN      
        1 tgagttaaat aactttaatt taataaaatt acccttatta tttgatgaag aatcaaatag
       61 attctgttag aattcgctaa tcctagatgt ttaaaggaat aacaataata aatcagtgga
      121 gaacgggata tgtttagttt gaaaaaaaca actgcggctg tatttgctct gggaagcagt
      181 gctttgtttg caggtacgat gggtccagta tgtacgccag gcaatgtgac tgttccttgc
      241 gaaagaaccg catgggatat tggtattacc gcactttatc tgcagccaac ttatgatgct
      301 gattggggct ataatggttt tactgatgtt ggtggctgga gaaattggca tgatgttgat
      361 ttagagtggg attggggctt taaattagaa ggttcttatc acttcaacac aggcaatgac
      421 atcaacgtca attggtacca tttggatgcc aacaccgatc attgggcgtt tgttgatcaa
      481 ttgcacgcct acgatattac ctgggatgca gtgaatgctg aactggggca atttgttgat
      541 tttagcgcga acaaaaaaat gcgcttccat ggcggcgttc agtatgccta catcaaaaca
      601 gacgttaatc gtcacttaaa cggcttcttt ctagataact tcaattccaa attcaatgga
      661 tttggtcccc gtaccggcct tgatatgaat tatgtattcg gtaatgggtt tggtatttat
      721 gcaaaatcag ctgtagcaat cctggtcggt acaagcaaat tcgtggataa ctgtactgtc
      781 tgtggttttt cttatggctc caaaaatgca atagtgccag aagttgaaat gaagttagga
      841 gcagattata cctatgcaat ggctcaaggt gatttgactc tggatgtggg ttacatgtgg
      901 ttcaattact tcaatgcctt acataatact gctgccgtga atgttggctt aggcacttct
      961 ctggaaaccg acttctctgc atctggcccc tacataggct taaaatatgt tggcaatgtc
     1021 taaataaccc caaattatgc tgctaatcta gtcatgatca agcagcagta tgcaaaaaac
     1081 ccgtattgac ataggctgct aatacgggtt ttttttattt ctctctgttt aattaagtta
     1141 gttatcctat ctaagctaac ttgtaattat atgataattt cccaatcaaa ttgagataac
     1201 tttatgcgaa aacaataatt ggtcgccctg ttgggcaaca tcaccaatga aaaatcacat
     1261 ccgtgatctg ctgcgaaaac tatagtaaac gcaataatct gctggttctg cctgatgcat
     1321 aattcttttc cattccaaag atcgtgggtt ataatgaaac catctatcac aaaccgttta
     1381 taaaaataaa catgcctgat gttctcggca aacctgatgc aacccagatt ccagatgacg
     1441 tctggcttga cattcttatc aattatcttc aaccacaaga tattgtaaac ctgtctgaaa
     1501 tcaacaatcg cttgagcaga cttttcaaaa ctcaacacac tcaatctgag caattgaacc
     1561 aaaaaaaaac attgggaatt ctgaaagtat ttgcaggata cagttccact ttcctgttcg
     1621 cacgcctcaa tggtaaacaa agattattta aaaccagaaa ctggagtgca gcagatagcg
     1681 ataacataaa cccaatatta atgcctatct ctctgccaga aaatatatct gttattgaat
     1741 ccattcaatc aacctacaaa cacactgtgg ttgttggtcg agataaacat gatcagatta
     1801 tagtagcaac aaataaaatt aatcaggatt atgcaaagca agaaaccagc cacaatcaac
     1861 tgattcccct tccattacgc gacattaaca tttctttggt gtctgccggg aatgaagagg
     1921 actatatttt aaaccctatt ttctcttttc ccgcaacaca gcagggcaat tgcatcttga
     1981 attgcatcaa ataaatggca atgacccatt gggaaccata attggggaaa ggaactacca
     2041 gttaccgatt ccattgcctt acactatgaa aacagtaaaa caaatacatt caggaatatg
     2101 gcatactgtg attagaggct atgatcaata taatcggccg caaatcgcta cttttggaaa
     2161 aaattatgat ggtcaattag gcacaggcga ttgtgaaaac cgggatacac ctaccccgat
     2221 aaagcttcct cctgaaatgc tgacgttcca tgccatccaa accggttttt ttcacaccat
     2281 tgccactgga cttgataaaa atggattccc tatcgttgca agctgcggtt ataactctga
     2341 tggccaattg ggatgcgggg ataaagataa taaaaacaca ttgattccca tcaaacttcc
     2401 agaaaatatg aaatcagtag aaatggtcgc cactggagta aaacacaccg ttattgtggg
     2461 aaaggacata aataacaagc ctgtggttgc aacctgtggg cataacggct atgggcagct
     2521 gggtacaggc gatgaagaga accggctctg cttaactccc atcgggattc ccgaggatat
     2581 tacaagtgtc gattatgttg gagcaggcgc ctatcataca gtcatttgtg ggcggaatat
     2641 aaaaaatcag ccaattataa ccttatgtgg gtgtaattca gacggtgaat tggggtttgc
     2701 accacaaaaa atcgccaaaa aatcatccct gatgaccaac ccagccatgt tcttcccacc
     2761 gcggcaagac aagagcgcaa tcaacaattt gatgaaaatt agtaaattct cgccatgcaa
     2821 tttgttatga ggtttaattg gtctccatag agaaaaatga agttctagaa tctcattatt
     2881 tctcctgtgc tttcttcttc tgttattttc aaccagcccc ctcccactta tacctatttt
     2941 aataaacatg tgtttctgga caaggtgaat taatcgctgc catacttaat gaattgaatt
     3001 tgtaaaaatg gaatgcatca tgccgacttc aaaaaaaatt cttatgattg taggagactt
     3061 taacgaagat ttggaagttt actttcccta tcaagcaatg ctcatgaaca attacctcat
     3121 tgatactgtt tcccccggca aaaataaagg agattttatt cagaccgctg tacatgattt
     3181 ggataaaccg catcttcaat cttacaccga aaaattaggg cacctattcc ccattacagc
     3241 ggatttctcc aaaatcactt taaaggatta tgacgcttta atcatcccgg gtggaagagc
     3301 tgctgaatat caacgtttga acaaagatat tttagctatt atcaggcatt ttcatgacgc
     3361 cgataaaccc attgcctgca tttgtcatgg catacaaatt ctcgctgagg ccggaatact
     3421 tgaagataaa aaatgtacga ctgtcggttt ttgtgaaccg gatgttcgaa aagcaggagg
     3481 acattttatc gatacaggga tggatggtgt tgtggttgat ggtaaattgg ttaccggagc
     3541 aacatggcta ggaaatgccc cctggatgag agcatttctt catatcttgc aaaatgaggg
     3601 ttaatcatct ttacagagtc tttgaccagt ggcaatcaga ttaagtcaac accaattcat
     3661 tgtatatata aaaattatat tcttaaacca tgattgttat cgtattccgc tgcaatggtt
     3721 gtttgtaaat caattgcagc cactttttta tagaattgcc cggtttctgg ttgcctttga
     3781 ttaaaaaagg tatgccgatt aagttgatta gacagttttg cgatttttat catagattct
     3841 aacgcacttt ctgccgttaa ctctccttgc ttatattttt tataatcatc aaatatcata
     3901 gcagcgccat taggtatatt aataactcta ccatcttcca gctggcgagt atttccaccc
     3961 aataaaccta attttccatc cagctgaatc acatcctgaa taatttcatc aattaaatca
     4021 tgagcagttt ttatcttctc gtcagatgag ttttctgacg ctaattggac agaattcttt
     4081 tctttagtac cttcttctcc aacagtgttc tgcgagataa tttcttgttc tttaacagta
     4141 gactcatgcg cttcaacttc tgttcccgct ccagaggctc ttgttaaacc taactcagct
     4201 acccttggtt ttaatgattc aaattgagtg aaacattgtt taatttgtgc tcttaacgtt
     4261 tcatttgaag taattctttg ccaaagctcc tgtgcgattt cctcagcacc agaccatttg
     4321 tttttgcagt gccattcatc ttgcctgggt ttaagttcta ttaaggctct gccaatctgc
     4381 tttaattcat gagctgttgg atttacagga tcagaaacct ggcttaataa ggtatgaaat
     4441 tttctgaggg gatctgagac ttctgaaagt ttaatagccc ctttaatgac tggtaatgtc
     4501 acaggattta attttttatc ctcatcaaca taatatagag tccattcccc agttttgtcc
     4561 ctgggtctaa tcaacaaata ggaatccggt tttagctcag gggatgtttc tgcagcaata
     4621 caccagctgt tttgttttac ttttaattca ttggcataat gatccaaatt aaataaacca
     4681 gtcttttgaa tatcaaaagc tatcaattga actgcatttt caagtctttc ttgaaaactc
     4741 atgttttcta tccccggcct tttgatactg ggaacccaac tggcgtcatt tgtattgttc
     4801 tgtggtacat aaaaatagtc tggctccttc acactaccat cgattaaata cctttgatct
     4861 tgtaattccc aatgaatcgc ttccatttca cttttgtcat tcaggattcc catttttttt
     4921 aactgatgaa ataaaaaccc tggaccagtc acacctaatg ttttagcaat tctctcccct
     4981 tttgtttcct tcattgatgc atattcatga gtgaactcaa aagaatctct cgcaatatag
     5041 gattgctcga tcgcatctgc taattgctgg attatttgat ttcctttgct acataaaatg
     5101 atatcattgc aaagagtatt attagctccc tgagaccagg gagacaatag aaacccaaaa
     5161 ggcgcataaa tttcatcagg gaaaggagca gagcataaga tatctgaatc caaataaatt
     5221 cctccctctg cagccaagat atttaatcgc aacatatcac tcaccatccc ataattagga
     5281 gattcctgat cgagttcatg acgtatgcta tcccggcaca caccttcttc attaatatct
     5341 tttaccgtga tgcctttgga tttcatgtct agaataaaaa gatcgagttc tttggcaggc
     5401 gctatttttt tatctaccca gataatcgtt tcatagccag ggttcttttc agcccattcg
     5461 gagacaacct ggatgttttt ttgaggcata atatgaccaa cccatataaa atggattttt
     5521 ttgtcaattt gagcctcttg accagcctgt ggtaaatttt ctgtcttcat atcaattctc
     5581 caacctagat atgaatatat tttatacaga ttgatattaa gcccggatga gcatcataag
     5641 aataaaactc atagtacatg ccagttaaga atagccagaa cctaacgaga gcgattaaga
     5701 aataaagaat aactacaagc tgatttccca ggtcctaacc catgagtgtc gatggatttc
     5761 cgtaacgatt atcaatataa ttttccggta aatttgtggc tttaatttgc taggaacaca
     5821 gcccaaacca ccaaggattg aatcatgaaa accttaacga cccgcatttt ctccctcatc
     5881 atgttattgg cgttgcattt taatccaatc gtttttgctc ataccaataa taccaataat
     5941 aaaggtttat cggcttccat taataatggt gtgggcagta gctcttctaa taacacgtat
     6001 gtcacacctc aagctttttg gaatttatat tttgatttca ccggagatga aactccagga
     6061 tatcccaaag gtaaaatcaa tatttcacaa accttatttc agtcagaaat gaaaaaaaac
     6121 agcagcttgg cgcagcaaaa tgaaggccaa ttaatcctgt tcatcaactc cacactttat
     6181 atttataata gtgaccgtca gcttaagcta aagcaattga tgcgcactgc gccaaattca
     6241 ggattcactg agatgactgc gatatcacac attggtccag ctctgatgta tctcgcaaaa
     6301 attaaagaaa atggcgatgc aagctggaaa tcacaaatgg aaaatttact taaggatatc
     6361 caggcagtaa aagtaatcaa tgcacaaaca cccaataatt ggctggagca agtcaatgct
     6421 ccagcctgga agcctcatct cactacaatc cataatatga ttgattatgc ctgttctatg
     6481 gcaggcaatt acatgagtga tgtattgaac gaaaaactat cgtttgatat ggcctcttta
     6541 caaaacgact ttcttaacgg caataaaacg tatcctattc cttataataa cgtcatgatc
     6601 ggcactttta tgttaaccgc cttgcaaagc atggatcaac ttcatagtaa aataagccaa
     6661 ttaaaaatag attggcctca tgcgaaggta attattcgtt ttgtcgcagg atctaatgtt
     6721 tcagctggcg taagcaaagg cagcaattgg ttggtcccat ttgttcaagc actatccaat
     6781 aataaattag ctacagatag aatttatatc acgccttatg ccgcagtgaa accatcgtta
     6841 ggcgcacaag agttaacaca agcagattac aactattata ataatacggt atggggcgca
     6901 agacataacc gtagaatcat cgccaacgaa gtctttacta acatcacgag catcttctta
     6961 ccagatcgac ctgctatacc cggtgattat acttattcca aaccacctaa aatcgaagat
     7021 tttctaatgc gcttaaagtt ttcattggca gaaccgactg aaatgttatc caatactgtt
     7081 gggttttgga tggctggtga acttgccgaa aaaaactgga attacaataa aatcagcatc
     7141 ccgggtatta ctactggttt cccagaaggc atatccacct atccgaacaa taatcctgta
     7201 atccagcgat gataaaaaaa attactcgca agtactattt tattgcttat atttcctatt
     7261 ttaggacaat ccaaaacggt acttgtcccc tatcaatatt taaaaacatc gggagcaaga
     7321 gatataacac cttttcactc gatagtgctc aataccttgc tatacctcag ttgtcaaaag
     7381 atgagccggg tattcctgcc aatatcaacg gcggcaatgc caaagttgat gtcctcattt
     7441 ttaagtggat taatgggaag ttccatttat atcaaaaaat cccggggcat ggtaatgaag
     7501 gcatagaata aaggatctaa cgtatctcgc tacctcctca atctattcaa aaccaaaaac
     7561 tgcaaataaa aagcatagtt ataccaaatt caaacagaca gcagcattta taaaataaaa
     7621 tagaatagcc caaagcaatg cttacaaagc aatcaaatcg ataagctctc gattctcaat
     7681 tagtatgccg tccggcaaag ggatcccgag tgaggccatg tatatcaggg attttgaaaa
     7741 atcactatac gaatattgcg aaagcagggg tgtacaattt aaaaaaggga cattctccgg
     7801 cctaagcgac aatttggttt cgtataaaga taccaaggaa aacatccgta cagtcaaatg
     7861 tgacatggta gttgattgtt ctggttctaa tcgtatggtt ctcaaagaaa ctactcatcc
     7921 cagacgaaat agatcccttg ctccaggcga aacaactatg caaattacga caaaaatatg
     7981 attggccaaa tttttgattt ccagtggtcg acatacgctc tgcaaaaaat gaagaaggtg
     8041 gcgttaatta ttttatttat tatgaagtac ccgataattt gaaagagaaa ga
//
LOCUS       DACTJE010000149         8043 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.149, whole genome shotgun sequence.
ACCESSION   DACTJE010000149 DACTJE010000000
VERSION     DACTJE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8043
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.149"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(75..896)
                     /locus_tag="I9760_002422"
     CDS             complement(75..896)
                     /locus_tag="I9760_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HAT5044181.1"
                     /translation="MRKLLIVFSIFLASCQTTHDKPDLTNKKRQTTHTKNSVYDRYKY
                     KSNRYTIQQDGAPKQAKQVSFKEPVPVKEPLSRYGNPSEYYVDGRTYEVMKSSTGYKT
                     RGIASWYGTKFHKQRTSSGEPYNMYVMTAAHKTLPLPTYVKVRNLENGKVAIVKVNDR
                     GPFHANRVIDLSYAAALKLGVFPKGTAHVEIETLMGPAGQGHYYLQAGAFSSEALAKR
                     LKEKLLHITPSPVFIEHYKQHYIVRVGPFGNKSMADNLKTKLSNNGIKGSFSVLI"
     gene            1092..2315
                     /locus_tag="I9760_002423"
     CDS             1092..2315
                     /locus_tag="I9760_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAT5044182.1"
                     /translation="MTRATSFLLTLLFFITLFVQSTNSMADEVLPSSDLQRPSPTVTT
                     KPFTTPAAPLLNAKAYILIDVNSGKVIAEKNSEEKLPPASLTKMMTLYVISNALKSGQ
                     IHLNDMVRVSRDAWQTGGSRMFIKEGDQVSVEDLLKGIIVDSGNDACVAMAEHLGGSE
                     NAFTDIMNQQAQNLGMKNSHFTDSTGLPDPNLYTTAKDLAILGRALILDFPEYYDWYK
                     QKWFTYNGIRQPNRNRLLWRDSQVDGIKTGHTNDAGYCLVSSAKRNNMRLLAVVLGEP
                     SDSVRADDSEKLLNYGFRFFETHQLYKSGQSIAELPLYKGTSDKVAVGLTEDQFITIP
                     AGQYERLNISTKISNDLQAPIKKGDKVGDIIVQFNNNIVSSKPLYALQTVESGGFYTR
                     TKDSIRLAFKRWFGS"
     gene            2328..3164
                     /locus_tag="I9760_002424"
     CDS             2328..3164
                     /locus_tag="I9760_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HAT5044183.1"
                     /translation="MSIAFVNGKYCCQSEAKISIFDRGFLFGDSVYEVLPVYHGQPYF
                     VDQHLDRLFSNMKKIKMIIPNYDWHGLIHRLISENNGGTLQVYIQVTRGNQGVRKHDI
                     PTSITPSVIAFTMHNPFPTLEDKEQGMSAKLVEDFRWMRCDIKTTSLIANILLNDEAV
                     SAGFHTAILARNGLITEGSSTNVFIVAQDGVIKTPPMNNFCLPGITRQVVIEIIKKLD
                     LKFREIEISISELFSAQEVWITSTTKEVFPITKINDSLINGGKVGEYWRIINDSYQQL
                     VN"
     gene            3168..3431
                     /locus_tag="I9760_002425"
     CDS             3168..3431
                     /locus_tag="I9760_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HAT5044184.1"
                     /translation="MTKTTLIEFPCYFPIKIIGINSELLLNEIRQIVINHFPTFENEH
                     LTHKTSEQNKYLAITVTVYAENQISLDGLYQDLSQHPAVKMVL"
     gene            3431..4030
                     /gene="lipB"
                     /locus_tag="I9760_002426"
     CDS             3431..4030
                     /gene="lipB"
                     /locus_tag="I9760_002426"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAT5044185.1"
                     /translation="MIIHNLGIKDYTEIWEQMKEFTAIRDSNSYDELWLLEHYPVYTQ
                     GQAGKPEHVLNPNSIKIVQSDRGGQVTYHGPGQLVAYVLMDIRRRNLGIRTLVAKLEE
                     ILISVLEHYRIPANIRSGAPGVYVGEKKIASIGLRVKNGCTYHGIALNVNMDLSPFLG
                     INPCGFAKMEMTQMSHFHPNIQLEEVSQHLVQYFLTQFK"
     gene            4040..6076
                     /locus_tag="I9760_002427"
     CDS             4040..6076
                     /locus_tag="I9760_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044186.1"
                     /translation="MNLLADYVQPLTYWLQANPHWSLFITFLIALSESLAVIGSIIPG
                     SVTMTAIGILAGSGIMRIDLTLLAATLGAICGDSLSYFLGYFYSDRLIEIWPFRKYPG
                     LLKYGKDFFASHGGKSVLIGRFVGPLRSIIPVIAGIMHMKQWRFLIANIVSAIGWSIL
                     YVMPGVLIGAAGHELSAEGATRFFILILVILAVIWLSSLVLKWLFIRLNSFLRSNLHR
                     FWLEIKTSPRLSKVYYAITPVQEENHYNTVGLLLIALVIFFLFLTLVIFTIEGQLLDY
                     INVPVHFFMQSFHSSLLEAVFIVCAQLTSKSTLTGLFVASCTWFIYHKNYRATVYLSS
                     ITLLSGVIALFLTKIIDSPRPQGLLVTLPGSSFPSVSISIATALYGFVLFYINSKYSL
                     ITSSFKTIILIVLGLSGFANIYLGDFWFSDILAAYLLGAEVCLIHCLVYRRFSTSVIK
                     NEHPLIMLISLLMVIVFATAVSTVVNFKEQVHDHMPYHKEFTLTEKQWWDQQRPLLPL
                     YRLSRIGKRISLLNIQYVGDLDALQNKLEQFGWEPQSDKTFFTRLLMRMNNAPSNVKL
                     PLLTQLYENKKPGLIMTYQDKKSKLIFDLTIWQSNYYLSEPKRIVWIGSIHQNIFNLK
                     QKKSRHNLPPVLNPLSYVIPALDDFTLRRIMLSDDLVKTSVFPTAPYILLIKTK"
     gene            6202..6807
                     /locus_tag="I9760_002428"
     CDS             6202..6807
                     /locus_tag="I9760_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system protein LssZ"
                     /protein_id="HAT5044187.1"
                     /translation="MHILAKVIHCLFPLIALTLLIIGIKRNAIYYIISSLWISLISLV
                     IHYQTSGGEILGTYFNYLNATIYTINLFVLCLSLVYVLWHLSNDNVAFKIVSSLFQSF
                     IVIGALLVVINLWINAYFIENRLNGTPVMQVALLEKPDYCNYRYIFYKVALDGTVYYL
                     CPNHYGLIPSIGRLSISPDFIASQLSIPSKKQMLLLQQKRS"
     gene            6946..7593
                     /locus_tag="I9760_002429"
     CDS             6946..7593
                     /locus_tag="I9760_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044188.1"
                     /translation="MAPKLNWDKAIDKAIKRYGLRVEPQLISYFSKAGVSYPPKEIAL
                     LAFKSERKVELWAKNPNQEWTHIHDYPLTGFSGRLGPKLRENDKQIPEGIYKLVNFNP
                     FSSMHLSMMINYPNSFDRQKGYMDGRKELGNNIFIHGKNLSVGCLAVGDLAIDQLFIL
                     ARRVGLKNIQVIIAPNDLRYQKPSTSTFAQPRWLPELYKQITESLKPFKKTKFTA"
     gene            7686..>8043
                     /locus_tag="I9760_002430"
     CDS             7686..>8043
                     /locus_tag="I9760_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HAT5044189.1"
                     /translation="MNTPRRQSWSHIDLGELAKNDSLLACLVLLTRYYQNPFSPPSLI
                     ARLPLVNSKLSVDMFSRAAARASLDSEVKTIKLEQITDSDIPLVLIKKNGEAVLLILE
                     DDGKKAILDPKQPDSRI"
BASE COUNT         2483 a         1340 c         1510 g         2710 t
ORIGIN      
        1 tttcccaaac tattctgagt ggttttttct taattctaaa gatataaatt aaccggtttt
       61 ttccacgaaa caaattaaat taacacagaa aaagatccct ttattccgtt atttgataat
      121 tttgttttaa ggttgtcagc cattgatttg ttaccaaaag gccctactcg gacaatatag
      181 tgctgtttat agtgttcaat aaacaccggc gacggtgtaa tgtgaagtag tttctctttc
      241 aaacgctttg ccagggcttc tgaagaaaag gctcctgcct gcaaataata atggccttgc
      301 ccagcaggcc ccattagcgt ttcaatttca acatgagcag ttcctttggg aaaaaccccc
      361 agttttaatg cggcagcgta cgagagatct atgacacgat ttgcgtgaaa tggccctctg
      421 tcattaacct taactattgc gactttgcca ttttccaagt ttcttacttt gacataagtc
      481 ggcaaaggta atgttttgtg ggcagcagtc atgacataca tgttataggg ttcgccactg
      541 gaggtacgct gtttatgaaa ttttgttcca taccatgagg caataccacg agttttatac
      601 cctgttgagc ttttcatgac ttcataagta cgcccgtcaa cataatattc agaaggatta
      661 ccatatctgc ttaatggttc tttgacaggc actggttctt taaagcttac ctgtttggct
      721 tgtttgggag ctccatcctg ctgtattgta taacgattgg acttgtattt atatcgatcg
      781 tagacagagt ttttagtgtg agtggtttgg cgttttttat ttgttaggtc gggtttgtca
      841 tgagtagttt ggcaacttgc taaaaaaatt gaaaaaacaa taagtagttt acgcatattc
      901 tttgatattt cttgttgtta tgagttattg ctaatattac caaaaaataa taattttcga
      961 agtgcgatta gattaattcg catcttaatt tagctttata aaaaaatata aatattttta
     1021 atatgccatt tggccgcctg caaaaggctg tgataagata catttatttt gcaactatat
     1081 caggtaatat catgaccaga gcaacgtctt ttttattgac gctattattt tttattactt
     1141 tatttgtaca gtctaccaat tcaatggctg atgaagtgct cccttcctct gatttacaga
     1201 ggccttcacc tacagtcaca acgaaacctt ttacaactcc ggctgctcca ttgcttaatg
     1261 ccaaagctta tattttaatt gatgtgaata gtggaaaagt aattgcagaa aagaatagtg
     1321 aggaaaaatt accacctgcc agtttgacta aaatgatgac attgtatgtc atttctaatg
     1381 cattgaagag tggacaaatt catcttaatg atatggtaag agtgagccgt gatgcatggc
     1441 aaacaggtgg ctctcgtatg tttataaaag agggggatca ggtctctgtt gaagaccttt
     1501 taaaaggtat tatagtagat tctggcaatg acgcttgtgt ggcaatggca gagcatcttg
     1561 gtggttccga aaatgctttt accgacatta tgaatcagca agctcaaaat ctgggtatga
     1621 aaaatagtca ttttacagac agtactggat tacctgatcc taatctatat acaactgcta
     1681 aagatttggc gattttaggc cgagccttaa tactcgattt ccctgaatac tatgactggt
     1741 ataaacaaaa atggtttaca tataacggta tacgccaacc taatcgaaat cgtttgttgt
     1801 ggcgagatag ccaggttgat ggtataaaaa caggccacac caatgatgct ggatattgtc
     1861 tagtatcttc cgcaaagcgt aataacatgc gtttattagc tgtggtttta ggggaaccca
     1921 gtgattctgt gcgagctgat gacagtgaaa aattattaaa ttacggattt agattttttg
     1981 aaacacatca attatataaa tctgggcaat caattgctga attaccgctt tacaaaggaa
     2041 cttctgataa agttgctgtg ggtttaacag aagatcaatt tattacgata cctgccggtc
     2101 aatatgaaag attaaatatt tcaactaaaa tttctaatga tttacaagct cctataaaga
     2161 aaggcgataa agttggcgat attattgtgc aatttaataa caatatcgtt tcaagcaaac
     2221 cgctttatgc tttgcaaaca gtggagtcag gtggattcta taccagaaca aaagattcga
     2281 taaggttagc atttaagcga tggttcggct cctaggatat tttcataatg agtatcgcat
     2341 ttgttaacgg caagtattgt tgtcaatctg aagcaaaaat ttcaatattt gatcgagggt
     2401 ttctttttgg tgactcggtt tatgaagtgc tgcctgttta ccatgggcag ccttactttg
     2461 tagaccaaca tcttgaccga ttattctcaa atatgaaaaa aattaagatg attataccaa
     2521 attatgattg gcatggttta attcatagac taatatcaga aaataatggc ggtactttac
     2581 aagtatatat ccaagtcaca cgagggaatc aaggggtgcg caagcatgat atccctactt
     2641 ccatcacacc ttctgttatc gcattcacta tgcataatcc atttcccacc ctcgaagata
     2701 aggaacaggg aatgtcagca aaactggttg aagattttcg gtggatgaga tgtgatataa
     2761 aaactacttc tttaattgcc aatatattac tgaatgatga ggctgtatct gcaggattcc
     2821 acactgcaat tcttgcccgg aacggtctaa ttacagaggg aagtagtacc aacgtattta
     2881 ttgtcgcaca ggatggtgtt attaagacac cacccatgaa taatttctgt ttaccaggaa
     2941 ttactcggca agttgttatt gaaataatta aaaaattaga tttaaagttc agagaaatag
     3001 aaattagcat ttcagagctt ttttctgctc aggaagtttg gataacaagt acgacaaaag
     3061 aagtattccc tattacaaag attaatgact ctttgattaa tggcggaaaa gttggcgaat
     3121 attggcggat aattaatgat tcctaccaac aactagtaaa ctaaattatg acaaaaacga
     3181 ctttaataga attcccttgt tattttccta ttaaaattat cggaattaac tcagaattat
     3241 tgctcaatga aatcaggcaa attgttatta atcacttccc tacttttgaa aatgagcatt
     3301 taactcataa aacgagtgaa caaaacaagt acttggctat tactgtgacg gtgtatgcag
     3361 aaaatcaaat ctcgctggat ggattatatc aggatttaag tcaacatcct gctgtgaaaa
     3421 tggttttata atgataattc ataatttagg cataaaagat tatactgaaa tatgggagca
     3481 gatgaaggag ttcactgcaa tccgcgacag taattcttat gatgagcttt ggttattaga
     3541 gcattatcct gtatatactc aggggcaggc agggaaacca gagcatgtac ttaatcccaa
     3601 ctctataaaa attgtgcaat cagatcgcgg tggacaagtt acatatcatg gcccaggaca
     3661 actagtagcc tatgttttaa tggatattcg ccgcagaaac ctgggcataa gaactttagt
     3721 agcaaaactg gaagaaatat taatttcggt tttagaacat tatagaattc cagcaaacat
     3781 tcgcagtggg gcacctggag tttacgtagg tgaaaaaaaa atcgcatcga taggactgag
     3841 ggtgaaaaat gggtgtactt atcatggtat agcattaaat gtgaacatgg atttgagccc
     3901 atttttaggc attaacccat gtggttttgc taaaatggaa atgacacaga tgagtcattt
     3961 ccatcctaat attcagttag aggaagtaag ccaacatctt gtacagtatt ttttgactca
     4021 atttaaataa taggaaatta tgaatttgtt agcagattat gtgcaaccac tgacttattg
     4081 gctacaagca aacccacatt ggtccctgtt tattactttc ttaatcgctt taagcgaatc
     4141 tttagctgtc attgggagta ttatacctgg ctctgttacc atgactgcaa tagggatatt
     4201 agcaggttct ggaatcatgc gtatcgattt gacattgctt gctgctactc tgggagctat
     4261 ttgtggagat agccttagtt attttttggg atatttctat agtgatcgtt taattgagat
     4321 ttggcctttt agaaaatacc cgggcttatt gaagtatggt aaagattttt ttgcaagcca
     4381 tggcggtaaa agcgtattaa ttggtcgctt tgttggccca ttgcgttcta tcattccagt
     4441 gattgctgga ataatgcata tgaaacagtg gaggttcctg atcgcaaata tagtttctgc
     4501 tatcggatgg tctattttat atgttatgcc aggggtgctg attggcgcgg caggacatga
     4561 attatcagca gagggagcta caaggttttt tatcttgata ttggtcattc ttgctgttat
     4621 ttggttgagt agcctcgttc tcaaatggtt atttattagg ttaaattcat ttttgagaag
     4681 taacttgcat cgtttttggt tagaaattaa gacaagcccc agattatcta aagtgtatta
     4741 tgctatcacc cctgttcagg aagaaaatca ttacaatacg gttggtttat tacttattgc
     4801 tctcgtgatt ttttttctct ttttaacttt agttattttt accattgaag gtcaactgct
     4861 agactatatt aatgtgcctg tgcatttttt tatgcaaagc tttcacagtt ccttactgga
     4921 agcagttttc attgtatgtg ctcaattaac aagcaaatca accttaactg ggttatttgt
     4981 cgcttcttgt acatggttta tttatcataa aaattaccga gctacggttt atttaagcag
     5041 cataacccta ttgagcggtg ttattgcttt atttttaact aaaattattg atagccccag
     5101 accgcaaggg ctattggtta ctttgcctgg ttcctctttt ccatctgtta gtatttccat
     5161 tgcaactgca ttgtatggtt ttgttttatt ttacataaat agcaagtatt ccttaataac
     5221 cagcagtttt aaaacaatta ttctaattgt gctcggatta agtgggtttg ctaatatcta
     5281 tttaggtgac ttttggtttt ctgatatttt agctgcttat ttgttgggag ctgaagtatg
     5341 tttaattcat tgtttggtat atagaaggtt tagtacttct gttataaaga atgaacatcc
     5401 tttaattatg ctcatatcac tgcttatggt tattgtattt gcgacagctg tttcaacagt
     5461 tgtgaatttt aaagagcagg ttcatgatca tatgccctat cacaaggagt ttactttaac
     5521 agaaaaacaa tggtgggatc agcaaagacc tcttttacct ttgtataggt taagcagaat
     5581 tggaaaacga ataagtctct taaacattca atacgtaggt gatctggatg cactgcaaaa
     5641 taagttagaa caatttggtt gggaaccaca gagcgataaa acttttttta ccagattgct
     5701 gatgcgaatg aataatgcac catcaaacgt taaactgcct ttactcactc aactatatga
     5761 aaataaaaag ccaggcttga taatgacgta tcaagataaa aaatcaaagt tgatttttga
     5821 tttaactatt tggcaatcga actattattt atctgagccc aaaagaattg tgtggatagg
     5881 ctccattcat caaaatattt ttaatttaaa gcaaaaaaaa tccaggcata atttaccgcc
     5941 agtattaaat ccattgtctt atgtaatccc tgctcttgat gactttaccc ttagaaggat
     6001 tatgctatct gatgatttgg taaaaacatc ggttttccct actgccccct atattctttt
     6061 gataaaaact aaatagcaga gcgtcattga ttgcaggaag catgttctga tttatttata
     6121 ttaggcccgg ctagtaaccc atgtaaatta ctgatataac tatgtatgtt cttataattt
     6181 ataaaataag ggatggaatc aatgcatatc ttggctaaag ttattcactg tctctttcca
     6241 ttaattgcgc taactctttt aattattgga atcaaacgaa acgcgattta ttatattatt
     6301 tcttccttat ggataagcct tatttcatta gtcattcatt atcagacttc cggtggagag
     6361 attttaggta cgtattttaa ttatcttaac gcaacaatat atactatcaa tttgttcgtt
     6421 ttgtgtttat ctttagttta tgttttatgg catttaagca atgataatgt agcgtttaaa
     6481 atagttagca gcttgtttca atcatttata gtcattggag ctttattggt tgtaatcaat
     6541 ctttggataa atgcctattt tatcgaaaac aggttgaatg gaacccctgt gatgcaggta
     6601 gcccttttag agaaacctga ttattgtaat tacagatata tattttataa ggtggcactg
     6661 gatggcaccg tttattatct ttgccctaat cattacggtt taatcccatc tattggtcga
     6721 ttgtctataa gcccagattt tatagcatca cagttatcaa taccaagcaa aaagcaaatg
     6781 ctcttattgc aacaaaaaag gagttaaatt agcgattttc tatattttaa caaattgttc
     6841 tgatatttat tatttgatag tatgtgtgta acttaccagt atagtgcgtc tatgaaacga
     6901 ttttttccga tattgtttat tctttactgc tgtaatttat ttgcaatggc acctaagctt
     6961 aactgggata aagcgattga caaagcaata aaacgttatg gactaagagt agaaccgcaa
     7021 ttaatttctt atttttctaa agctggggtt agctaccctc ctaaagaaat tgccttgctt
     7081 gccttcaagt cagagcgtaa agtcgagtta tgggcaaaaa atccaaacca ggaatggacc
     7141 catattcatg attacccatt aactggcttt agcgggcgtt taggacccaa attacgtgag
     7201 aatgataagc agatccccga agggatctat aaattagtaa attttaatcc ttttagcagc
     7261 atgcacttat ccatgatgat taactaccca aatagtttcg atcggcaaaa aggttatatg
     7321 gacggtagga aagagttagg aaataatatt tttattcatg gaaaaaattt atctgtagga
     7381 tgtttggctg tcggtgacct tgccattgat caactgttta tcttggcaag aagagttggc
     7441 ctcaaaaata ttcaggttat tattgcccct aatgacttac gatatcaaaa gccatcaaca
     7501 tctacatttg cccaacccag atggttgccc gagctttata agcaaataac agaatcactc
     7561 aaacccttta aaaagactaa atttactgct taatataaag attagtcttt ttattatttt
     7621 atttaagtaa tttgagtata tacttaaata aaataaataa tataaagaat aatcataaaa
     7681 atcatatgaa tactccgaga agacaatcat ggtctcatat tgatttgggt gaactagcca
     7741 agaatgactc tctgctagcc tgcctggtat tattaactcg atattatcaa aatccgtttt
     7801 cgcctccatc acttatagct cgtttgccat tggtcaatag taaattgagt gttgatatgt
     7861 tttcgcgtgc cgctgccaga gcctctttgg attctgaagt caaaactata aaattagaac
     7921 aaatcactga ttcagatatt cccttagtat tgattaaaaa aaatggcgag gctgtgcttc
     7981 taatactgga ggatgatggt aaaaaagcaa tccttgatcc caagcaacct gattcccgga
     8041 ttg
//
LOCUS       DACTJE010000150         7934 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.150, whole genome shotgun sequence.
ACCESSION   DACTJE010000150 DACTJE010000000
VERSION     DACTJE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.150"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..771)
                     /gene="lem4"
                     /locus_tag="I9760_002431"
     CDS             complement(<1..771)
                     /gene="lem4"
                     /locus_tag="I9760_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem4/SmdA"
                     /protein_id="HAT5044190.1"
                     /translation="MTTSVSFDETSKRNEGRVKNIVYLDFDGTITGAHGKEVISSPLC
                     EALSTKATFDERMRYKNEYDASDNKIKITENAKNFLQDVNKLHPQVKIVIISRNHENY
                     IKALLEFENIDHRNIIIYPRGVGNTIGPGEDKYKAVVSHEEKPECLPGFRLICDDDEV
                     DGEEMCNGLIHTGRSQLVKFHNEKPGQFKWGEYFKEILTNCDIAVKEYLNGKIGSRFH
                     LFTAKVDEKTGDQSFKDRYSQVKGDILKRAIINNLKNDI"
     gene            complement(1260..1862)
                     /locus_tag="I9760_002432"
     CDS             complement(1260..1862)
                     /locus_tag="I9760_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HAT5044191.1"
                     /translation="MKKSIERSSDTLLEIASHQELKGEVTYQRILQVLGERAFGIVLL
                     FFALPSALPFSFIPGISLIFSVPILLFAFQMVFARKTMWLPKIIAERTIHQETMAKFI
                     HNTVPYLIKIEYFLKPRWLFMTSRFMEIIHGIVIFFLALLLMLPIPFSNFIFATLLII
                     FSLGLIEKDGLFLIIGYLGVVIYANFVYLFVLTAVKHLFG"
     gene            complement(1985..2443)
                     /locus_tag="I9760_002433"
     CDS             complement(1985..2443)
                     /locus_tag="I9760_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044192.1"
                     /translation="MEHSGYDNNGKLSIMGIICLIIFLVLFFFSVFIMPFLIWELHYN
                     VPDFVSNMIAYLEDTYYYSSSVSKTLVWLTFFIPCLITGYFSYYISRSIDKKNLLKDA
                     NIDEEQSKEISSVTNEHIKESASLGFKILILMAAIILIIFLLQEFVKFTS"
     gene            complement(2588..2779)
                     /locus_tag="I9760_002434"
     CDS             complement(2588..2779)
                     /locus_tag="I9760_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044193.1"
                     /translation="MLGHGGLKKVIALMLSVGSIFILAACTISSNADNVLQADGQSAS
                     HEHQKHKGHGGYGGGVGGR"
     gene            complement(3320..5107)
                     /locus_tag="I9760_002435"
     CDS             complement(3320..5107)
                     /locus_tag="I9760_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAT5044194.1"
                     /translation="MNGLEKQCCPKDVILTQQEKASESLSVCCAPSILGASDSFFSFF
                     HKLFQANLAKMTLGISPAALGSSYSTWLWQLAQSPEVLWELALYPFLHANDCINNIIC
                     VERAADGKDVRFKKDSWQPMPWRLLAEGFLQVEDWWRRATNIPGLPRQVERTVSFWVR
                     QCLDSVSPSNFVWSNPDLFHEAIRTNGLNLLKGSQIAMEDWLEKLTGAPPTGSENFIP
                     GKDVAVTAGRVVFQNHLIELIQYEAQTKTVYKEPILILPAWIMKYYILDLSPHNSLVK
                     WLVKQGHTVFIISWRNPDKEDCDLGMDDYYRLGAMAAIDMVSTILPETKINLMGYCLG
                     GTLAMITASMMGRYEDERLNCLTLLAAQGDFSEAGELMLFVNESQVDFLKSMMWEQGY
                     LDTKQMAGSFQMLRAYDLIWSKMVQDYMHGMRRGMIDLTAWNADATRMPYKMHSEYLE
                     KLFLNNDFAEGRYMVEGKSVAAENIQCPIFAVSTKKDHVAPWQSVYKIHLMTEENVTF
                     VLTGGGHNAGIISEPGHSGHAYHVHERKKGDAYLNPTNWLEIAEKREGSWWREWHDWL
                     VQQSTKKRILPPVLNTSLPSAPGTYVLQK"
     gene            complement(5270..5416)
                     /locus_tag="I9760_002436"
     CDS             complement(5270..5416)
                     /locus_tag="I9760_002436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044195.1"
                     /translation="MTAKEKQLSLDSLQDFEAQVGEEFIDKVNGQRGFIGNARTRKKK
                     RSFL"
     gene            complement(5500..>7934)
                     /locus_tag="I9760_002437"
     CDS             complement(5500..>7934)
                     /locus_tag="I9760_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAT5044196.1"
                     /translation="GRGYIKSTDDIEKIAIGTNTNGTPILLRDVATVQLGSDMRRGVV
                     ELNGQGETVGGIVIMRFGEDVLQVISHIKEKLKELRAAIPEGMKIVNVYDRSNLIREA
                     ISTVNWNLIEELVVVGLLIIVFLGHFRSALIPIITLPLAILISFIPIYFLGIGLNIMS
                     IGGIIVAIGDMVDAAIIMVDNAHKRIADWEMKGCSGDRTQVLIDSAKEVGPAIFSSLL
                     VIAISFMPVFTLEAQEGRLFSPLAYTKNLAIFMSALLAITLIPMLLPLLVRGRIIPEQ
                     KHPITRFMQKMYAPVLRLALRYRKFVMGIAVVAALSIIPLYKLIGSEFMPPLYEGTIL
                     YMPVTLPGISVTQATSLLQEMDKRLKTFPEVAHVFGKTGRAETSTDPSPFNMMEVIVE
                     LKPKEFWRKGMTYESLVAEMDKALQFPGVSNAWTMPIKARNDMLTTGIRTAVGIKIFG
                     PDIKKIEAIGKEIEMVAKGVLGTSNVYAERVAGGYFLDFQINRDQLARYGLTIMDVNR
                     IIESAVGGENIATTIEGRERYPVNVRYLRELRDDPDKINRILVKTPSGAEVPIAQLVT
                     LTFRSGPAMIRDENAMLAGYVFIDISGRDIGGYVEELKQVVKAKVKLPPGYTLAWSGQ
                     YEFMQRVYERLKIFVPLTLVIIFVLFYFTFRTITETLMVMLGVPFALFGGFLLLYVLG
                     YNMSIAVWVGMIALAGVAAETSAVMLAYLDSDYNKQKEKGLLKTLPDLIQMVQQCAVS
                     RIRPMVMAGLANILGLLPVMWATGIGADVMKRLAAPMVGGVFSALLLTLIVIPVVYVI
                     WRWQADLRKQTH"
BASE COUNT         2534 a         1722 c         1316 g         2362 t
ORIGIN      
        1 tatatcattt tttaaattat taattattgc gcgttttaat atatctcctt ttacttgact
       61 gtatctatct ttaaaagatt ggtcacctgt tttttcatct acctttgctg taaaaagatg
      121 gaagcgtgaa cctatttttc cgttcaggta ttcttttaca gcaatatcgc aattagttaa
      181 tatttcctta aaatattcac cccatttaaa ttggcctggc ttttcgttat ggaattttac
      241 aagttgggag cgtccagtat gtataagccc attacacatc tcctccccat ctacctcgtc
      301 atcatcacaa atcaaacgaa atcctggtaa acattcaggt ttttcttcgt gtgataccac
      361 tgctttgtac ttgtcttccc ctggccctat tgtatttcct acgccgcgag gataaataat
      421 aatatttcga tgatcaatat tttcgaattc taaaagagct ttaatataat tttcatgatt
      481 acggctgatt attacaattt ttacctgtgg atgtaattta ttgacatctt gtagaaaatt
      541 cttggcattt tctgttattt taatcttatt gtcagatgca tcatactcat ttttatagcg
      601 catcctttca tcaaaagtcg ctttagtact caaagcttca cacagggggg aactaatcac
      661 ttctttgcca tgtgcaccag ttattgttcc atcgaaatcc agataaacga tgttctttac
      721 cctaccttca tttctttttg aagtttcatc aaaagacacc gacgtagtca tgattatttc
      781 ctcgcaaaac cacccaatgc atatatttaa tcattatgat ttgtttatgt acaattaatt
      841 gttttcataa gtcatattat ttttaataat atccattgtt tttcaatagt ttaatgatta
      901 tttgtgcgct tgcgctcagc taaatatggg ttgcattgaa agaaaaaaga actaccagcg
      961 aaaacataag cttcctcaac tattttatgg ggtttactat ttcgatttgc tgcaaaaaac
     1021 aaagaccatc tacccatatc atcttattcc gttaatactt gatgttagcc aggcattcca
     1081 ttatatgctg agactccagg atagcaaggc atcttaattc ggttatgtga gaacctgaaa
     1141 aattactgca tcaaagttat tccgagtgat taaagtggaa ttttttattt atttacatat
     1201 ggttaattca actataatga aaaaagtgaa ctctaatggt tagtaacaca agctttcaat
     1261 caaccaaaca aatgcttcac cgccgtcaag acaaacagat agacaaagtt tgcataaatc
     1321 acaaccccta gatacccaat aatgagaaat aatccatctt tttctattaa tcccaagcta
     1381 aaaataatta acaacgtagc aaaaataaaa ttactaaatg gaattggtaa catcaataat
     1441 agggcaagga agaaaataac aatcccatgg attatttcca taaagcggga agtcataaac
     1501 aaccatctgg gttttaaaaa atattcaatt ttaattaagt aaggaacggt attatgtata
     1561 aacttggcca ttgtttcctg gtgaatggtt cgttccgcaa taatttttgg tagccacatt
     1621 gtttttctgg caaaaaccat ttgaaatgca aataataata ttggaacact aaaaatcagg
     1681 gagataccag gaataaatga aaaaggcagc gcactaggca acgcaaaaaa taaaagcaca
     1741 attccaaagg cacgctctcc aagcacttgc aaaatacgtt gataagtaac ttcccccttt
     1801 aactcctgat gacttgcgat ctctaaaagg gtatcagaag agcgttctat gctttttttc
     1861 ataaaaaatc caagtcaaaa tatatagcat caatttacca actgtttgag gaaaattttt
     1921 accttaagaa tttttaaagc tcacactttc tctaactctg cagaaaacac agaggtactt
     1981 caagttatga tgtgaattta acgaattctt gtaataagaa aatgattaaa ataatagccg
     2041 ccattagaat aagaatttta aatcctaaac ttgctgactc cttaatatgt tcattagtta
     2101 ccgaagaaat ttcttttgat tgttcttcat caatgtttgc gtctttcagc aaatttttct
     2161 tatcaataga gcgagaaatg taataagaaa aatacccggt aatcaagcaa ggtataaaaa
     2221 aagtcaacca gacaagagtc ttacttacag acgaagaata ataataggta tcttcaaggt
     2281 atgctatcat attgctaaca aagtcaggaa cattataatg taattcccag attaaaaaag
     2341 gcatgataaa gacactaaag aaaaagagaa ctaaaaaaat aatcaaacaa attattccca
     2401 ttatagataa tttgccattg ttatcatacc cactatgctc cataggttcc ttccatcaaa
     2461 tgcccagtta actaaaatta ttctttttta aattttaaaa tatcagcatc cataatataa
     2521 ctggatatat tttaagcaaa tttttgaaaa ttagcttaaa gaaaaataaa ctgattccca
     2581 gttgcttcta acgtcctcca acaccacccc catatccccc atgcccttta tgtttttgat
     2641 gttcgtgtga agctgattgt ccatcagcct gtaaaacatt atccgcgttt gatgatatag
     2701 tacaagcagc caaaataaat attgagccta cggataacat taaagcaata actttcttca
     2761 atcctccatg accaagcatc gtaaaactcc tgttaccttt tgattaaaat tgataagtgt
     2821 tttataaaaa aatattgctc ttattatcat cggaatttgg gggttattat ttatctaata
     2881 acattaagta tagtctttgt aatgctttgt gccatttgta gttttcttca aaaaaatatc
     2941 tcgtaataca tgtggttctt tagtcacggc ttgagttttg cgcttaatct ttagtacata
     3001 ttgacatttc ccctgaccca gcatatgcca ttactgtgac caaatagaga tgaattttgc
     3061 gcaaaatcaa attcattcaa aaatagaaat gcctcttcaa ttgctgttgc agtaaactca
     3121 agttctgaat tgctgagact ttaaaatatt catccttcaa acgtagaatc catgtgagct
     3181 agatccatat taccagacat ggcagccaat aaattgatat cattttacac cagagttttg
     3241 cgtaaagttc attgactacc tactgccagt cattaaagat cacgctcttc aagaatttct
     3301 tagccatacc cattcacttc tatttctgca acacataggt tccaggagct gacggaagtg
     3361 atgtattcaa tacaggtggt agaatgcgtt ttttggttga ctgttgcact aaccaatcat
     3421 gccattctcg ccaccaagag ccttctcgtt tttctgcaat ttcaagccaa ttcgtgggat
     3481 ttaagtaagc atcgcctttc ttgcgctcat gaacatggta agcatgacca gaatgacctg
     3541 gctcactaat gatacctgcg ttatgcccgc cacctgtaag gacaaaagtc acattctcct
     3601 ctgtcataag atgaatttta tataccgact gccaaggtgc tacatggtct tttttagtac
     3661 ttacagcaaa aataggacac tgaatatttt cagctgccac ggattttcct tccaccatat
     3721 accgcccctc ggcaaaatca ttgtttaaga acaatttttc aaggtattca ctgtgcattt
     3781 tataaggcat ccttgtggca tcagcattcc aggctgttaa atcaatcatc ccacgacgca
     3841 tgccatgcat atagtcttgc accattttag accagattaa gtcatatgcc cgcagcatct
     3901 ggaaagaacc agccatttgc ttagtgtcca gataaccttg ttcccacatc atacttttaa
     3961 ggaaatccac ctgactttca ttcacaaaga gcattaattc gccagcttca ctaaaatccc
     4021 cttgggccgc aagtagcgtg aggcaattta aacgttcatc ctcatatctt cccatcatag
     4081 acgccgtaat catggccaag gtaccgccta aacaatagcc catcagattg attttagttt
     4141 ctggtagaat ggtagagacc atatcaatgg ccgccatggc accaagacga taataatcgt
     4201 ccattcccaa atcacagtcc tctttatctg gattacgcca tgaaatgata aatacggtgt
     4261 gaccttgttt tacgagccac ttcactaaag agttatgtgg ggataaatca agaatatagt
     4321 atttcataat ccaagcaggc agtatcaata tgggttcttt gtacactgtc ttggtctggg
     4381 cttcatattg aatgagttca attaaatgat tttgaaatac cacacgtcct gctgtcacag
     4441 ccacgtcttt acccgggata aaattctcag aacctgtggg tggtgcacca gttagctttt
     4501 ctaaccaatc ttccattgct atttgactgc ctttgaggag attaagcccg ttggtgcgaa
     4561 tggcttcatg aaataaatcg ggattggacc aaacaaaatt agaaggagac acggaatcaa
     4621 gacattggcg tacccaaaac gaaactgttc gctccacctg ccggggtaat cctgggatat
     4681 ttgtagcacg tcgccaccaa tcttctacct gcaaaaatcc ttccgccagt aaacgccaag
     4741 gcatgggctg ccaactatcc tttttaaaac gcacgtcttt accatcagca gcacgctcaa
     4801 cgcaaataat attgttaatg cagtcattgg catgcaagaa tggataaagg gcaagctccc
     4861 ataagacctc tggagattgc gccaattgcc aaagccaggt ggagtaagac gagcccaacg
     4921 ctgcaggact aatacctaat gtcattttag caagattggc ttgaaatagc ttatggaaga
     4981 aagagaagaa actatctgaa gcacctaaaa tactaggagc gcaacaaacg ctcaaacttt
     5041 ctgaagcctt ttcctgttga gtcagtatga cgtctttggg acaacattgt ttttccaatc
     5101 cattcatggg ccgatatcct tggcattatg aaacaaaata taggttgaga tatttttaaa
     5161 tccaccttat ctttaaataa gtgtaacgta ttctggataa tcttccatct tgcccaaaac
     5221 tcagggcaaa ccagtttatc gaggttccca ttattcataa tcttattcgt cacaaaaaag
     5281 agcgtttttt cttccttgtt ctggcattac caataaaccc acgctgacca tttactttat
     5341 ctataaactc ttccccaacc tgcgcttcaa agtcttgcaa cgagtcaaga gataactgtt
     5401 tttcttttgc agtcatctat tgcaacttac cacgtaaacc ttcattcaca tgctgtttac
     5461 agatgttaac aaacaaatcg atcatattaa cagtgttggc taatgggttt gttttctcaa
     5521 atctgcttgc caccgccata ttacataaac cactgggata acaattaagg tcagcaatag
     5581 tgctgaaaaa acaccaccca ccatgggggc tgcaagccgc ttcatgacat ccgcaccaat
     5641 gccagttgcc cacatcaccg gtaacaaacc gagtatgtta gctaatcctg ccatcaccat
     5701 agggcgaata cgtgacaccg cacactgttg aaccatttga attaaatcag gcaaagtttt
     5761 gagtaatccc ttttcttttt gcttgttata atctgaatca agataagcaa gcatcaccgc
     5821 acttgtttcc gcagcaactc ctgcgagagc aatcatgcct acccaaactg caatactcat
     5881 gttatatccc aatacataaa gcagcaaaaa gccaccaaat aaggcaaacg gcacacccaa
     5941 catgaccatc aaagtctctg tgatagtacg aaaggtaaaa taaaacaaaa caaaaataat
     6001 gactaaagtc agtggtacga aaattttaag tctctcataa actctctgca tgaattcata
     6061 ttgacctgac cacgcaagtg tgtagcctgg gggcaatttg actttggcct taaccacttg
     6121 ttttagttcc tccacgtagc caccaatgtc acggccgctg atatcaatga acacataacc
     6181 tgctaacatg gcattctcat cgcgaatcat ggcaggtcct gaacggaaag tgagagtcac
     6241 taattgagca ataggcactt cagcgccact gggtgttttg actaaaatgc gattgatttt
     6301 gtctggatca tcacgtaatt ctcgtaaata acgtacatta acaggatatc gctcgcgccc
     6361 ctcaatcgtg gtagcaatgt tttcacctcc cacagccgat tcaataatgc gattgacatc
     6421 cataatggtt aaaccataac gagccagttg atcgcgatta atttgaaaat caagaaaata
     6481 ccccccagct acccgctcag catagacatt acttgtaccc agcactcctt tagcaaccat
     6541 ctcaatttct ttgcctatag cttcaatttt tttaatgtca ggaccaaaaa ttttaatccc
     6601 aactgctgtt cgaattcctg tggtgagcat gtcgttgcgt gccttaatgg gcatggtcca
     6661 ggcattactc actccaggaa attgcaacgc tttatccatc tcggcaacca ggctttcata
     6721 agtcattcct ttgcgccaaa attctttagg ctttaattca acaatcactt ccatcatatt
     6781 aaatggggaa gggtcggtgg aggtttctgc tcttcctgtt ttcccaaaaa catgtgccac
     6841 ttcaggaaac gtttttaatc tcttatccat ttcctgcaac aaagaagtcg cttgggtcac
     6901 agaaataccc ggcaaagtga cgggcatata taaaattgtc ccctcataaa gaggaggcat
     6961 gaactcagag ccaatgagtt tataaagagg aataatgctc aaagcagcta ccacggcaat
     7021 acccatcaca aattttcgat atctcaaggc taatcttaac actggagcgt acattttttg
     7081 cataaaacgg gtaataggat gtttttgttc aggaatgatt cgaccacgca ctaaaagagg
     7141 caagagcata ggaatcaaag ttatcgcaag caaagcgctc ataaagatag ctaagttttt
     7201 ggtataagct aaaggtgaaa acagtcgccc ttcttgtgct tctagagtaa aaacaggcat
     7261 gaaagatata gcaatgacca aaagagagga aaaaatcgca ggccccactt ccttcgccga
     7321 atcgatcaac acttgtgtac gatcgccaga gcaccctttc atctcccaat ccgcaatacg
     7381 tttatgggca ttatcgacca tgatgatagc cgcatcaacc atatccccaa tggctacaat
     7441 aatgccgcca atggacataa tgttaagacc aatacctaag aaatagatgg gaataaatga
     7501 aatcaaaatg gcaagtggca gggtaataat aggaatcaaa gccgaacgaa aatgccctaa
     7561 aaacacaata atgagcaaac ctactaccac caactcttca atcaaattcc aattgacagt
     7621 ggagatggct tcacgaatta gattgctgcg atcataaaca ttgacgattt tcatcccttc
     7681 aggaatggct gcccgtaatt ctttcaactt ctctttgatg tgactaatga cctgtagcac
     7741 atcctcacca aagcgcatga tgacaatacc gccaactgtt tctccttgac catttaattc
     7801 gacaacacct cgacgcatat ctgagccaag ctgcacagta gctacatcgc gcagcaaaat
     7861 cggtgtccca tttgtattgg tacctatagc aattttttca atgtcatctg ttgacttaat
     7921 ataccctcgc ccac
//
LOCUS       DACTJE010000151         7933 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.151, whole genome shotgun sequence.
ACCESSION   DACTJE010000151 DACTJE010000000
VERSION     DACTJE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.151"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..535)
                     /locus_tag="I9760_002438"
     CDS             complement(<1..535)
                     /locus_tag="I9760_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAT5044197.1"
                     /translation="MIPEKLLTYTKTIFEYSHSTVYIKDKNFHYQYMNNRGLKQLQLQ
                     EKDIMGMPDKALPFGKYAHFYNSHDYSAMEEIIYFQLDDCITKNGEKIFALSHKLPLK
                     DEDGKTHGVIGFTKFMNLHQLMNNLNSKNTFLTPNLKINVNVDDWLKTTINLSRREME
                     VLYYFLQGKSIKLIAPLL"
     gene            783..1271
                     /locus_tag="I9760_002439"
     CDS             783..1271
                     /locus_tag="I9760_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAT5044198.1"
                     /translation="MDFEQSLWTIPSSHTKNALVMKIHLTPLTQSLFELLKAETNSSF
                     VIPGLNDEEPLTDKAIAKAVSRIQTRVGIPHWTAHDLRRTFATQLGEALHVDPVVIEK
                     CLGHKMPKIMATYNKNEMLPQRKEALIRWEERIKQLIGSDSLKLAVYCKTALNKAEIT
                     IG"
     gene            1278..2735
                     /locus_tag="I9760_002440"
     CDS             1278..2735
                     /locus_tag="I9760_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT5044199.1"
                     /translation="MIKNLITQQEGKTLEFKRDLSSPKSLLKSIVAFANTAGGHLIIG
                     VNDERQIIGVEHPLDEEERLCNLIADSISPRLIPSIELVTLNNKTLLVIEVFVSNTGP
                     HWINSEGSTQGIYVRLGSTNRQADQELITELHRRSEGVNFDEMPMPQLSVDDVDITKA
                     QELFSDIKSLNEKNLLTLKLITSHQGKLVPTKGAILLFGKERNTYFPDAWVQCGRFIG
                     KDKSRIFDHVDIYDYLPDAVESIMMFLKKHALRGADFSAVQRKDIWSIPLVMLREAVI
                     NALVHTDYSQRGAPIRISFFDDRIEIENPGILLPGLTIEDMRQGISKIRNHVIARVFR
                     ELNLIEQWGSGVSRIYSEAIEQKLQEPEIIEIGMRIRFIIYLAQGIPIDSTFDKRDKQ
                     LESRLESRLESRLESKLAARVIQVLQNGEAGKIQIARSLGHQTVSGELHKQIKQLLKL
                     EIIEMTIPDKPNSRLQKYRLTNKGIDLINIIQREL"
     gene            complement(2915..3502)
                     /locus_tag="I9760_002441"
     CDS             complement(2915..3502)
                     /locus_tag="I9760_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044200.1"
                     /translation="MKTYTKLANIKSLLRKPVFTSKEANELGMSPSLLCYYVQKDIIH
                     RVSKGLYQAVYVKKEFDFRWEDLIFTLRSISQGVICLVSALAIYELTDEMPRKHWIAV
                     PHSTTKPMRPNTIVKRMRDVSTGLTTWNVDGESVPIFDMERTIIDAFRFLSKEIALKA
                     LQTGLREKHIDSRKLQRYAKKLRVDVTPYLLAMTI"
     gene            complement(3671..4273)
                     /locus_tag="I9760_002442"
     CDS             complement(3671..4273)
                     /locus_tag="I9760_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044201.1"
                     /translation="MVENLCYYLFIRQNKSNIGLYFSTLRYHLIRMKVGIMDDLLDQI
                     ETGIQNNLYYLSLFVSLAIPDICGALESVNGVASGSKYASWFDKNVVIPNGYYLNGMD
                     CYYFRCSMLHQGKTTHKNSNFTRILFVEPSSSNIFHNNIINKALNIDIKIFCNDIINA
                     TKCWISVISTDKNFIKNYKDFVRRHPKGLPPYISGVPVIS"
     gene            complement(4512..5189)
                     /locus_tag="I9760_002443"
     CDS             complement(4512..5189)
                     /locus_tag="I9760_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT5044202.1"
                     /translation="MSIREKIGKRIHEARKAKGLTRQALADLTDDIKPSRINNWEHGT
                     RMPGPEEITQLAQALEVSPSFLMGLSDERDGDFGINSRVGSLIPLLDHKQAGDPKYHI
                     QRVKEEPYAEKITFLPVNQDIANQLDEYAFALKMLDDSMSPELRINDIQIVDPSISPN
                     PGDFVVVKISGKDDVIICQYKKISYTSSEFELLTLNDNWPNITFNESEQVEIVGTVIQ
                     NIRTYRR"
     gene            5403..5609
                     /locus_tag="I9760_002444"
     CDS             5403..5609
                     /locus_tag="I9760_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAT5044203.1"
                     /translation="MEHTKNKPFKQKLLFIKDVEQIIGRNRLTLRRWWSAGKFPTPTK
                     LNGTVLVWQTDVIEQWINLHTESK"
     gene            5647..5955
                     /locus_tag="I9760_002445"
                     /pseudo
     CDS             5647..5955
                     /locus_tag="I9760_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215740.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein LvrA"
     gene            6759..7867
                     /locus_tag="I9760_002446"
                     /pseudo
     CDS             6759..7867
                     /locus_tag="I9760_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISLpn8 family transposase"
BASE COUNT         2623 a         1433 c         1401 g         2476 t
ORIGIN      
        1 gaagcaatgg tgcaataagc ttgatcgatt ttccttgtaa aaaatagtac aaaacctcca
       61 tttctctccg agacaaatta atcgttgttt ttagccaatc atcaacatta acattgattt
      121 taagattagg tgttaaaaag gtattttttg aattaaggtt attcatcagt tgatggagat
      181 tcataaattt cgtaaagcca atgacaccat gagtttttcc atcttcatct ttaagcggta
      241 atttgtgaga taaggcaaat attttttcac cgtttttagt aatacaatca tctaattgaa
      301 agtaaataat ttcttccatt gccgagtaat catgactatt ataaaaatgc gcatatttcc
      361 caaagggaag tgctttatca ggcataccca taatatcttt ttcttgtaat tgcaattgtt
      421 tgagtcccct attattcata tactggtaat gaaaattctt atctttgatg tatacggtac
      481 tatgggagta ttcaaaaatt gtttttgtat aagtcaagag tttttcaggg atcataaagc
      541 tattgtgaca caatcttttt ggttgaaaat aataccacaa gattataaat aaatcaattt
      601 acttttttat tagatcctga actacttatt ttaattaata aatcttggat tgattgtatt
      661 attggataaa aatgacccaa tctcgctttg taaaagatgt gatttccctg gatgaattta
      721 gctgtaaatt gactattatt gttaacctca atagaattgc catctaattt atcatcaatg
      781 aaattgattt tgagcaatca ctctggacaa tcccatcaag ccataccaaa aatgcgctgg
      841 ttatgaaaat tcatttaacg ccactcactc aatctttatt tgaattatta aaagctgaaa
      901 ccaattcttc ttttgtcata ccagggctta atgatgaaga acccttaacc gataaggcta
      961 ttgcgaaagc agttagcaga attcaaactc gagttggcat tcctcattgg acagcccatg
     1021 atctaagacg cacctttgca acccaacttg gagaggcttt gcatgttgat ccggtggtaa
     1081 ttgagaaatg tttagggcat aagatgccta agatcatggc tacttacaat aagaatgaaa
     1141 tgctacctca aagaaaagaa gctcttattc ggtgggaaga acgtattaag caacttatag
     1201 gatcagactc cctcaaatta gctgtatatt gcaaaactgc tttaaacaaa gcagaaatta
     1261 caattggata aaaattcatg ataaaaaatc taattacaca acaagaagga aaaacactcg
     1321 agtttaagag agatttatca tcaccaaaat cactacttaa atctatcgtt gcttttgcaa
     1381 atacagctgg cggtcacctg attattgggg tcaatgatga aagacaaata ataggtgttg
     1441 agcatccatt agacgaagag gaacgtctgt gtaatctgat tgctgattct atctcaccac
     1501 gtcttatacc tagtattgaa ttggtaacac taaataataa aacactgctg gtaattgaag
     1561 tatttgtcag taatacagga cctcattgga tcaattcaga aggatcgaca caaggtattt
     1621 atgtccgact aggttccaca aatcgtcaag cagatcaaga gcttatcaca gaattacacc
     1681 gtcgctcaga aggagtaaat tttgatgaaa tgcccatgcc tcagctttct gtcgatgatg
     1741 ttgatataac gaaagctcaa gaactatttt cagatattaa atcgctgaat gaaaaaaacc
     1801 tcctgacttt aaagctcatt acttctcatc aaggaaaatt agttccaaca aaaggtgcga
     1861 tattgttatt tggaaaagaa cgcaatacat attttcctga tgcctgggta caatgtggcc
     1921 gtttcattgg taaagacaaa tcccgtattt tcgatcatgt tgatatctat gactatcttc
     1981 ccgatgcagt tgagagcatc atgatgtttt tgaaaaagca cgctttacgt ggcgctgatt
     2041 tttcagcagt acaaagaaaa gatatttgga gtatccctct tgttatgttg cgcgaagctg
     2101 ttatcaatgc acttgtccat accgactact ctcaacgtgg tgcgccaatt aggatctctt
     2161 tttttgacga ccgtatagaa atagaaaacc caggaatcct gttacctggg ctaacgattg
     2221 aagatatgcg acaaggcata tccaaaatta gaaatcatgt tattgccaga gttttccgtg
     2281 aacttaattt aatcgaacaa tggggaagtg gtgtaagtcg tatctattcc gaagctatag
     2341 aacaaaaatt acaagaacca gaaattatag aaattgggat gcgaatacgc tttattatat
     2401 atcttgcaca agggatacca atcgattcca ctttcgataa aagggataaa caattggagt
     2461 cacggctaga gtcacggcta gagtcacggc tagagtcaaa acttgcggct agagtcatcc
     2521 aagtactgca aaatggggaa gctggaaaaa tacaaattgc tcgaagttta ggtcatcaaa
     2581 cagtatcagg agaactacac aagcaaatta agcagctact aaagcttgag ataatagaaa
     2641 tgactattcc agataaacca aatagtagat tgcaaaagta tcgcctgact aacaaaggga
     2701 tagatctaat aaacatcata cagcgggaat tgtaatgctc accctaggtt tttctcaagg
     2761 ccaaaataat aaaaatcaga caaaacctct tcacttatca ataacagatt agtatttgtc
     2821 tgattttaaa aaagctcaag gatttatttt aataaattat cattttctat aaagattaat
     2881 ataacttttt tgtccgcttt aagcgattgt tcattcatat cgtcatcgcc aacaaataag
     2941 gagtaacatc aacgcgcagt ttctttgcat agcgttgcaa ctttcgagaa tcaatatgtt
     3001 tttctctcaa tccagtctga agtgccttaa gagcaatttc ttttgaaaga aatcgaaagg
     3061 catcgataat agtacgctcc atgtcaaaaa tgggtactga ttctccatca acattccatg
     3121 ttgttagacc agtgcttaca tccctcattc tttttacaat ggtatttggc ctcattggtt
     3181 tcgttgtact atgaggtact gctatccaat gcttacgagg catttcatct gtaagctcat
     3241 atattgccag tgcgctaacg agacaaataa caccttggga aattgatctc agtgtaaata
     3301 tcaaatcttc ccacctaaaa tcaaactctt tcttcacata aacagcttga tataggcctt
     3361 tgctaactcg atgaataata tctttttgca catagtaaca gagcaaactt ggagacatac
     3421 caagttcatt agcctcttta ctggtaaaaa cgggctttct cagaagagat ttaatgttag
     3481 caagttttgt atatgttttc atatctatat tttaataaat aaacccataa aaatctaatg
     3541 ggtttattta ttaaaactgt ttactaaagc cgacacaaac ccgacacact tttgttctgt
     3601 aaaaccctgg gaatttcaac gattgatgtt aaaatccagt cattcccaat tttagttctg
     3661 aaaggttaaa ttaagatata acaggaacac cacttatgta aggaggaagt cccttcggat
     3721 gtcttcttac aaaatctttg tagttcttta taaaattttt atcagtactt attacactta
     3781 tccaacattt tgttgcgttt attatatcgt tacagaaaat ttttatgtcg atatttaatg
     3841 ctttgtttat aatattgtta tggaaaatat tagaagaact tggctctacg aaaagaatac
     3901 gggtgaaatt actattttta tgagttgttt ttccttggtg aagcatagaa catctaaaat
     3961 aatagcaatc cattccattc aaataatagc cattcggaat cacaacattt ttatcgaacc
     4021 aacttgcata tttagaaccg ctagccacgc catttactga ttcaagtgct ccacaaatat
     4081 caggaatagc aagactcaca aataaggata gataatacaa gttattttga attcctgttt
     4141 caatttgatc tagcaaatca tccataattc ctactttcat tcttatcaaa tgatatctta
     4201 atgtagaaaa gtatagacca atattcgatt tattctgcct tataaaaagg tagtagcaga
     4261 gattttccac cacaaaccaa tgcttttttt atttcctgat taaattagag cagcaagacg
     4321 tagagcaaat aagtttaatt atcgcaaata agtatataat aggtataaaa ttggtataaa
     4381 ttatacctat tttgattaat gtttccggaa tagtggttta tgctaatatg agtcaaattc
     4441 ctcttccatc ctgagtaata cccagctttc ccctggaatt tcaccccatc ttaaaaatct
     4501 tattggaaat tctaccgacg atacgtccga atattttgaa tcaccgtccc cactatctcg
     4561 acttgctcac tttcgttaaa agtaatattg ggccagttat cattcaaggt cagtagttca
     4621 aactcagatg aagtatagga tatcttttta tactgacaaa taatgacatc atctttgcca
     4681 cttattttga cgacaacgaa atcaccagga ttgggggata tagaagggtc aactatttgt
     4741 atgtcattga ttcttaattc cgggctcatg ctgtcatcaa gcattttcaa agcaaaagca
     4801 tattcatcta attgatttgc tatgtcttga ttaacaggta aaaaagttat tttttcagcg
     4861 taaggttctt ctttaactct ttgaatatga tatttcggat caccagcttg tttgtggtcg
     4921 agcaatggta ttaatgaccc gactctagaa tttatgccaa aatcaccatc tcgttcatcg
     4981 gataacccca tgagaaaaga tggggatact tctaaagctt gcgccaactg cgtaatttct
     5041 tcgggtcccg gcatccttgt gccgtgttcc caattattta ttcgagaggg ttttatatca
     5101 tcggttaaat cagctaaagc ctgcctggtt aagcctttag ctttacgcgc ttcatgaata
     5161 cgtttcccaa tcttttctct aatgctcatt acaataaatt caccaaaatt gaaaaaagct
     5221 ctgattgata cttgattgtt acacgaaagg tgttatattc aataatattt gcacgtaacg
     5281 tgtaatttaa tgtgttttac ctcttaaact taacacaaaa tgagtaagtt aacgagcggg
     5341 aatttggaaa tgactgcata catacaacaa aacgtttgca gtactgatta tggagttact
     5401 atatggaaca tacaaaaaat aaacccttca aacaaaaatt actctttatc aaagacgtag
     5461 agcaaattat tggccggaat agactgacat taagaagatg gtggtctgct ggtaagtttc
     5521 ctacaccaac gaaacttaat ggtactgtat tagtatggca aacagatgtt atcgaacagt
     5581 ggattaatct gcatactgaa tcaaaataaa tacatctatc aaaaaaattc ttaggaagag
     5641 tgaataatgc attttttatt tgtaaatgct caggaacttg aagcgcttat ggaattaccc
     5701 tatattcaac gtttaaccta tctcatgggc attcgcccct atatggacag agaaacgcat
     5761 atagttggga taaagcgtag aatcagttat caatcactac gagaggcgtt atacgttgcc
     5821 cctattgcag gtgtaaaaac aggcagccca agccagcaac aggtaaagcg agcagttaaa
     5881 tcgttagaac gtgctggttt gattgaaatc aaatccacaa cacggcatct tattttaaaa
     5941 tgccttttag ccgaagggca tcaatctttt ccaaaacagg ccgaccctag gccgaaccct
     6001 aaagccgcca taaacccgac acacttttgg ccttttaaaa tatagggatt ttctaatatg
     6061 cattgtaaaa cccatcatgc ctataagcgt ttaggccgac ccacctcaga actcagaatt
     6121 attatttatt tatatatata tttttgttta tttgaaaaaa ttttctttaa aagtgatcca
     6181 cagaaaaatt tataaatggt gctttaattt tagtgagtat aagtgactac acagatcaac
     6241 agcgtttagg aaaaattcgg tgcagcaatt gcaatgtgtt ttatgatcta gaagaattaa
     6301 ttaccctacc atttaaaggc gtcactccac tattaaccca ttaataaaag tttctatgct
     6361 cttcacttca tgatgaactc atgatgaata agtgatgcag aagttatatt tatcccagat
     6421 agacactaga tcttccgtgc atcacctaat tcatcacaca ttcaccgcta ttttgagctg
     6481 tgtactacca gttttttggt aaaatattat tattaatcat atggatatgg cgcatattta
     6541 tatcatgcga aaggtgtgtg tgttatctta acatttatta aaaattattt tgtcttcaga
     6601 ccttatttta tatttataaa tcttcaaatt tttaagtgca gacaattgca cgtttgtgat
     6661 tgataatatt tatatagttg tctttgtaac catcccctaa aatagttgta ttcctgatta
     6721 gagttctcca agttaaacta ccactaagga gaacgaagat gaaaaaatcg cgttatacag
     6781 aaactcagat tgtcaaaatt ctaaaagagg ttgaagcagg cagactggtt aaagaaattt
     6841 gtcgtgaata tggtatttct gacgcaacat attacaactg gaaagcaaaa tatggaggca
     6901 tggagtcatc agacataaag cgtctgaaag accttgaaga agagaaccgt cgtctaaagc
     6961 agatgtacgc tgaactgagt cttgatcaca aaatcctgaa ggatattgtt gaaaaaaagc
     7021 tgtgaagcca actgtgcgtc gggagttggt tgattatgca gtcaatgctc atgctgtgag
     7081 tttacgccgc gcttgcaagg ttgtgggtat caacgactcg gtttatcgct ataagccgga
     7141 tagtcggtct gatgaaagtg tcattgtggc attacaagaa tcatctgaac gttacccagc
     7201 gtatggcttc agtaaattgc ttaaggtctt acggcgccag gggcacaaat ggaatcataa
     7261 acgggtttac cgtgtgtatt gtgagcttaa gctcaatatg cgtcgcaagg gaaaaaagcg
     7321 gttacccaat cgctcacctg gacctctgag tgtgccagcg tccattaacc agtgttggtc
     7381 aatggacttt atgtgcgatg cattgatgtg tggtcgacgc ttccgaacat ttaacatagt
     7441 ggatgatttc aatcgtgaag tgttagcgat cgagatagat ttgaacctgc ccgcacagcg
     7501 agtaattcgc gtattagaac gtgttgttgc ctggcgtggt ctccctgcca aactacgtat
     7561 ggataatggt cctgaattta tttccagagc cctagctgat tgggcagaaa aaaataaggt
     7621 gcagttggaa tttatcaagc cagccaagcc aacacaaaat tcatttattg agcgattcaa
     7681 tagaacttat cgcacagaaa tactggatat gtatgcgttc aaaaatttac acgaagtaag
     7741 agagttaaca gaaaactgga taaaggagta caacgacgaa agaccccatg attcactaaa
     7801 tgatttaacg ccgtgggaat atctggcaaa aaatcagcca atgaactcta atttaggatg
     7861 ccactaaata tggggggttt acagctacaa tttatgcaac aaattcaaaa tagcttattc
     7921 ctcagtacta tca
//
LOCUS       DACTJE010000152         7918 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.152, whole genome shotgun sequence.
ACCESSION   DACTJE010000152 DACTJE010000000
VERSION     DACTJE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.152"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            95..1942
                     /locus_tag="I9760_002447"
     CDS             95..1942
                     /locus_tag="I9760_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HAT5044204.1"
                     /translation="MKSTDPIKIAVLPGDGIGIEVTEATLPVFEVLDVPVILNYGDIG
                     WEFWKKEGAAIPSRTWQLIASSDTVLLGAITSKPQREAKQELSNALKKSNPYYVSPVI
                     QLRQGLDLFANVRPCFSIDDQSKPFNFCIIRENSEGLYCGFDYFPLPKAIHSLLAESQ
                     HWQTIPADEASCALRLQSKSGLTRLFDFAFKHAMQTGMPRVTLADKPNVLRESGEFTR
                     KIFESTAQRYPKIQADILNVDAVALWLIKSPEKFGVIVAENMFGDILSDVGAGVMGGL
                     GLAPSANIGDKGSYFEPVHGSGPRIRKNCANPSAMFLTISMLLNHFGYPDRAKKIVNA
                     VMQVIKEKRFITYDLGGHATTTDMANAVIEHCARLNASCLSKDFNPTPKENLIESDTM
                     PNLLQQLINCNSAEISDALDACGIEGGLLSIKPLSQGMKIIGPAYTIQYLPREKKGTA
                     FHNAANYIDKVPKHSVIVIDNNGQIDCTVWGDLLTHTALRNNIMGTVVHGAVRDVESI
                     RSTNYPVFCTGIYMCSGKNRVYKANEQCPLSINGVTINPGDIIFADDNGVLVIPNDRL
                     QEVVNKTIAIRLTEERIKTAIASGSTLEQAREDYCYEQPWLGINKKRES"
     gene            1942..2802
                     /locus_tag="I9760_002448"
     CDS             1942..2802
                     /locus_tag="I9760_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044205.1"
                     /translation="MFSPEKLKFIDIHHHAAPDLYIRRRNAIEAGKLYHSLYGAVVLK
                     SHIGSTGVQATIAQEMGLPVFPSVVLNHINGGINYRVVIRALSEYQPLFSGKMIVDFP
                     TITGRKLQSKLSRELTHDNLKQDTQKGETLFGSKQQLRKEVIDILKMTRDYPIVLTSG
                     LASRDEIYRLIDACNQYDVPSLLLSQPSNPLIGLKFTELNELIKNEWLWVEQTALTFI
                     LGYQEKQDFSQILTHLPRVIYSSDLGQLGQMDIPEWLHFSKKIFNELDLSAKRMEELT
                     LSNAIKLLNL"
     gene            complement(2967..3683)
                     /locus_tag="I9760_002449"
     CDS             complement(2967..3683)
                     /locus_tag="I9760_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAT5044206.1"
                     /translation="MLVTKEVEYEDGDTVCKGFIAYHSSASKPLPCVMVAHDWGGRGE
                     GACNKARQLAGMGYIGFAIDMYGNAKLGKDKAEKRALMTPFRENRGKLITRINAAFNK
                     VVQLPEVDSEKVAAIGYCFGGLCVLDLIRSGAPLKGVVSFHGVLLPLEGESNKSLNAK
                     VLILHGYDDPLVPIEQVNQFAIEMTERKVDWQVHVYGQTAHSFTDPGANDDEMGLHYN
                     KLADQRSWQSTQLFLQELFA"
     gene            complement(3815..6961)
                     /gene="dotA"
                     /locus_tag="I9760_002450"
     CDS             complement(3815..6961)
                     /gene="dotA"
                     /locus_tag="I9760_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotA"
                     /protein_id="HAT5044207.1"
                     /translation="MNKLAITVLLCFFPALALAENGALSFAPPASDLSVVFLGNLFGV
                     VDGVLHGTGSQIMGNMFGVFNSAVLALGGIIIMYTLMVSTMNTAHEGQMLGQKWSSIW
                     IPLRSTFGLALLIPKASGYCMMQVFFMWVIVQGVGAADKIWEAALSYLNRGGVIIQAQ
                     ADPTKSLQAAGSSSSGVAKGALTILGGQICMLGLQKQLQAQRDLYLSQSKSPPCGGNP
                     TPEMNTFCRTAIPDFISTVNFVKKQNDDTPKDLTANQPASFELDMPNFDKSSPFYFLN
                     GICGTVKWNNISALNSTNQSDNKGLVTVGGAGSNSSMGANSLNITSSQLQTARLSRAI
                     AIQQMYVTLSTVAQVMVNNDPAFSTTTSTGNSKNDFSAIAKQQFGVPYKSSGEVCTEY
                     QQVCQTWGSVPSSTGSTTGVLFNGTEFLGAINDYNGIMMPTLNLIRQATSKEFDKKSR
                     DFIAEANAKGWIMAGSYFFDLVKLNGSATEFADQFDTGTGLDKSSFDPTQLTKPFGKT
                     CQDPYSLLCTWFQNKSDKLIQIQSLIDGVPALGQDGVKQPDLSDNPQRQSVSGPLSST
                     VYGFVNNSMMVQLPGQPGIKPLTFANLINFKVDTSLYYMKHQDFDCGRVKILFFSFCL
                     GRMMGDLFYNYVFRYVYNFFLAIFGEMINSIVMAFLMIPLQGMKDIFIVGVQTLTQPG
                     INPIVALANMGTMYINFSGTLWLTLLNMAVVSSLIPLFGIFIFALIMMAMPLLMAWIG
                     TMVSIGFVTAYYIPVLPYMIFTFGSFAWLIAVIEAMVAAPIVALGVTHPEGNEAFGKG
                     EFAIMILVNVFLRPSLMIIGYIAAIALSYVGVWILNAGFDHAISYIQSDQGIETWEKG
                     TSSNDAKRFFQSAGNWTGAKMDTSQWDNFKDKLTGDYQSTALEGGYTGWAGVYAFFFS
                     ILIYTSMYLIIVQKAFTLIAHLPDKVLRWIGGSPESFGQETMQWGEEAKGRVEKAGEA
                     TYGAEKAIGDKLGAKGQEMLGKLGPQAKKISENAGDVSGKGKNSGGSQTGPSSKPDTG
                     QQLGGSDSGTPTSTPPPE"
     gene            complement(6958..7413)
                     /locus_tag="I9760_002451"
     CDS             complement(6958..7413)
                     /locus_tag="I9760_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein IcmV"
                     /protein_id="HAT5044208.1"
                     /translation="MKKKSGSRIVRVFTRIINVRKWFDWDRMKSLTLYLVNGIKRLFI
                     PQEPTHIESFDEAARKLKLSEADLVIKQKALFRLSIIMVVAAFMILIYTGYQFLYGSW
                     KATIISLVVVMIALVLAFRYHFWYYQIKQRKLGCTVKEWYRQGLLGEKE"
     gene            7526..>7918
                     /locus_tag="I9760_002452"
     CDS             7526..>7918
                     /locus_tag="I9760_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAT5044209.1"
                     /translation="MPDLSHEASAKYWFEYLDPMIYRVITFMESVENWTLDGNPELEE
                     AMKQLGQELDDIEKIDLGLLAEEDKFIRIVGNIKSGRGLRLLQAIDTVHPGSASRVLI
                     HAEETSLSSSDPAGFFLKRNIVFERLRLL"
BASE COUNT         2445 a         1700 c         1490 g         2283 t
ORIGIN      
        1 tgtggccaat tgattgtagt tcccgaagca agtatgcgag tcctagaaag caaccccact
       61 ttttgcaaaa aaattatatt taaggccctg aatcatgaaa tccactgatc ctataaaaat
      121 tgctgtatta ccaggtgacg ggattggtat tgaagtgact gaggctacac ttcctgtttt
      181 tgaagtcctt gatgttcctg taatcttgaa ttatggtgat attggctggg aattttggaa
      241 aaaagaagga gcagcaatcc cctccagaac atggcaattg atagcctcct ctgataccgt
      301 tttgctagga gcgattacca gcaagccgca acgtgaagcc aaacaggaat taagcaacgc
      361 gcttaaaaag agcaatcctt attatgtttc tcccgtcatt cagttaaggc aaggtttgga
      421 tctttttgcg aatgttcgtc cctgtttttc cattgatgac caatcaaagc cgtttaattt
      481 ttgtatcatt agagaaaata gcgaaggatt gtattgcgga tttgattatt ttccattacc
      541 aaaagcaata catagtttat tagcagaaag ccaacattgg caaacgattc cagctgatga
      601 agcaagttgt gccttaagat tgcaatcaaa atccggttta acccgtttat ttgatttcgc
      661 ctttaagcat gccatgcaaa ccggcatgcc tagagtcact ttagcagata aaccaaatgt
      721 tcttagagaa agcggcgaat ttaccaggaa aatttttgaa agcacagccc aaagataccc
      781 gaaaatccag gcggacatac tcaatgtcga tgccgtagca ttatggttga taaaaagccc
      841 ggaaaaattt ggagtaatcg tcgcggaaaa tatgtttggt gatattttat ccgacgtcgg
      901 agcaggagtc atggggggat taggccttgc acccagtgct aatattggcg ataaagggag
      961 ttattttgag ccagtacatg gaagcgggcc aagaataaga aaaaattgtg ccaatccatc
     1021 agctatgttt ttaacgatta gcatgctgtt aaatcatttt ggttatcctg accgagcaaa
     1081 aaaaatagtc aatgccgtaa tgcaagtcat aaaggaaaag cgttttatta cttatgatct
     1141 gggtgggcat gccactacga cagatatggc gaatgcagtg attgaacatt gtgcgcgatt
     1201 gaatgcctct tgtttatcaa aggattttaa tcccacccct aaagaaaatt taattgagtc
     1261 tgatactatg cccaacttgc ttcagcaatt aataaattgt aattccgcag aaatttctga
     1321 tgcactggac gcttgtggaa tcgaaggcgg attattgagt ataaaaccgt tatcgcaggg
     1381 aatgaaaatt attgggcctg cttatactat ccaatactta cccagagaaa aaaaaggaac
     1441 agccttccat aatgccgcaa actatataga caaggttccc aaacattcgg taatagttat
     1501 tgacaacaat ggtcaaattg attgcaccgt ttggggtgac ctattaacac ataccgcatt
     1561 gagaaataac atcatgggca ctgttgtgca tggggcagtg agagatgtcg aatcaattcg
     1621 ctcaacaaac tatcctgttt tttgtacggg aatttatatg tgctctggta agaatcgcgt
     1681 ttacaaagcc aatgaacaat gccctttatc cattaatgga gttacaataa atcctggcga
     1741 cattatcttt gctgatgaca atggagtctt ggtgatccca aatgatcgcc ttcaggaggt
     1801 agtgaataaa acaattgcca taagattaac tgaggaacgc attaagaccg ctattgcatc
     1861 aggttcgaca cttgagcaag ctcgagaaga ttattgctat gaacaaccct ggttaggtat
     1921 taataaaaaa agagagtcgt agtgttttcg cctgaaaaat tgaaattcat agacatacac
     1981 caccatgcgg cccctgatct ttatattcgt cgcagaaatg caattgaagc gggaaaattg
     2041 tatcactctt tatatggtgc agtcgtttta aaaagtcata ttggcagtac tggtgttcag
     2101 gcaactattg cgcaagaaat gggtttgccc gtttttcctt ccgttgtgtt aaatcatatc
     2161 aatggaggaa taaattatag agtagtcatt cgcgccttga gtgaatatca acctcttttt
     2221 tcaggtaaaa tgattgttga tttcccaact atcacaggtc gtaaacttca atcaaaacta
     2281 tcaagagaat taactcatga taatttaaaa caagatactc aaaagggtga aacactgttt
     2341 ggatcaaaac aacaactacg caaagaagtg attgatattt taaaaatgac ccgggactac
     2401 cctattgttt taacttcagg acttgcctcg agagatgaga tttatagatt gattgatgcc
     2461 tgtaatcaat atgatgttcc ttcattatta ttaagccaac catctaatcc tttaatcgga
     2521 ttaaaattca ccgaactcaa tgagttgatt aaaaatgaat ggttatgggt ggaacagact
     2581 gccttaactt ttatccttgg gtaccaggaa aaacaagatt tttctcaaat attaactcat
     2641 ctccctcgag tcatttacag ctcggactta ggacagctcg gtcaaatgga tattccagag
     2701 tggctgcatt tctcaaaaaa aatatttaat gaattggatt tatctgcaaa gcgcatggag
     2761 gagctgactc tctccaatgc catcaaatta ctcaacttat aaagttccaa tcaatctatt
     2821 gcctaagctg cctctttcgt tttattgctg ctttgcaaac gtttctatgg tgctcatttt
     2881 ctgcagataa cctcttctcc tcaaaacgtt tgcgtcttgc tccaaaacaa aataaccagc
     2941 tgatgtaaaa gtctaatatc aataaatcat gcaaataact cttgcaaaaa caattgagtg
     3001 ctttgccagg aacgttgatc cgctaattta ttgtaatgca gtcccatttc atcatcattc
     3061 gcaccagggt cagtaaacga atgagctgtt tggccataaa catggacctg ccagtccact
     3121 ttcctttctg tcatttcaat agcgaactgg ttaacttgct caattggtac taagggatca
     3181 tcataaccat gtaatatcaa tactttcgca tttaatgact tattactctc accctctaaa
     3241 ggtaataata ccccatgaaa actgacaact cctttaagcg gtgccccgga acgtatcaag
     3301 tccaatacgc acaaaccacc gaaacaataa ccaatagcag ccactttctc actatccact
     3361 tcaggcaact gcaccacttt gttgaaggcc gcatttatac gagtgattaa cttccctcta
     3421 ttttctctga aaggagtcat taaagctctt ttctctgcct tatccttacc tagcttggca
     3481 tttccataca tgtctatagc gaatccaata taccccatgc cagcaagctg tctcgctttg
     3541 ttacaagctc cctctccacg ccccccccaa tcatgagcaa ccatgacaca aggtaatggt
     3601 tttgaggcac tggaatgata ggcaataaaa cctttgcata cagtatcacc atcctcatat
     3661 tccacttcct tcgtgactaa catattaatc ctccttttta aaaggctgtc caaacagcat
     3721 taatagctac ctgtcaaagt tttaataaaa tgaactaccc atatataata agaagggctc
     3781 ctgcgagccc ttcttattat attgtttttg aatgttattc gggaggtggg gtactggttg
     3841 gggtaccact atcagaaccg cccagttgtt gacctgtatc aggtttcgag cttggtccag
     3901 tctggctgcc tccactgttc ttacctttcc ctgaaacatc acccgcattt tccgaaatct
     3961 ttttagcttg aggccccaat ttgccaagca tctcttgccc tttagccccc aatttgtcac
     4021 caatagcttt ctcagcccca taagtagctt cgcctgcttt ctctactcta cctttggctt
     4081 cctcacccca ttgcattgtt tcttgaccaa aagattccgg actaccacct atccatctca
     4141 gcactttatc tggcaggtga gcaatcagtg taaaggcttt ttgtacaata attaaataca
     4201 tactggtata aattaagatg gagaagaaga aagcatacac tcctgcccag cctgtatacc
     4261 caccttctaa tgcagttgat tgataatcac ctgtcaactt atccttaaaa ttatcccatt
     4321 gcgatgtatc catctttgct ccagtccagt tcccggcgct ctggaaaaac ctcttcgcat
     4381 cattcgaact ggtgcctttt tcccatgttt ctattccttg atccgattgg atgtaggaaa
     4441 tggcatgatc aaatccggca ttcaaaatcc acacgccaac ataagacaag gcaatcgctg
     4501 caatatatcc tattatcatc agagaaggtc ttaaaaagac attaaccaaa atcataatgg
     4561 caaattcacc tttaccaaaa gcctcattac cttctggatg cgtaacaccc aaggcgacaa
     4621 taggtgcagc caccatagcc tcgataacgg ctatcagcca ggcaaatgat ccaaaagtga
     4681 aaatcatata aggtaaaaca ggtatataat atgctgtaac aaatcctatg gataccatgg
     4741 tccctatcca tgccatcaac aaaggcatag ccatcataat caaggcaaag ataaagatac
     4801 caaaaagtgg tattagtgaa ctgaccactg ccatattcaa cagcgttagc cacaaagtac
     4861 cagaaaaatt aatatacatc gttcccatat tagccaatgc gacaatgggg tttattccag
     4921 gctgtgtcag cgtttgcact cccactatga aaatatcttt catgccttgt aaaggaatca
     4981 ttaaaaaggc catgacgatg gaattaatca tttctccgaa tatggctaaa aagaagttgt
     5041 acacatacct gaaaacatag ttataaaata agtcacccat catacggcca aggcaaaacg
     5101 agaagaacaa aatcttcacc ctgccgcaat caaaatcctg gtgcttcata taataaagcg
     5161 acgtatctac tttaaaatta atcagattgg caaaggtcaa cggttttatt cctggttgac
     5221 ctggcaattg aaccatcatg gaattgttga caaatccata gactgttgag gacaatggcc
     5281 cactcacaga ctgtcgttga ggattatcac taagatcagg ttgcttgacg ccatcctgac
     5341 ctaaagcagg tacgccatcg attagtgact ggatttgaat cagcttatca ctcttgtttt
     5401 gaaaccaggt acacaataat gaatagggat cctgacacgt ttttccaaag ggtttggtaa
     5461 gctgtgtggg atcaaaacta ctcttatcta ggcctgttcc cgtatcaaat tggtctgcaa
     5521 actcagttgc gcttccattg agtttcacca aatcaaaaaa gtaagagcca gccatgatcc
     5581 atcctttggc attggcttct gcaataaaat cacgggattt cttatcaaat tctttagacg
     5641 tagcttgtct tatcagattg agagttggca tcataatgcc attataatca ttaattgcac
     5701 ctaaaaattc ggtgccatta aacaatactc ctgtagtaga tccggtggaa ctgggtaccg
     5761 acccccatgt ttggcaaact tgttgatact cagtgcaaac ctcaccggat gacttataag
     5821 gaactccaaa ctgctgtttg gcaatagcgg aaaaatcatt ttttgagttt ccggtagacg
     5881 tggttgttga aaacgccgga tcattattaa ccatgacctg tgccacagta gacaaagtca
     5941 catacatttg ctgtatggca atcgcgcgag acaatcgggc tgtttgcaat tgagaagaag
     6001 ttatatttaa gctgttagcg cccatggatg aattggatcc agcccctcct actgtcacaa
     6061 gccctttatt atcactttga ttcgttgagt tcaaagcgga aatattattc catttgactg
     6121 tgccgcaaat cccgtttaag aaataaaatg gtgaactttt atcgaaattt ggcatgtcca
     6181 gttcaaaaga agcgggctgg tttgccgtta gatctttagg cgtatcatca ttttgtttct
     6241 tgacaaaatt taccgtactg atgaaatcag gaatcgctgt gcggcaaaaa gtattcattt
     6301 caggagtagg attaccccca caaggtggac tcttactttg actcaaataa agatctcttt
     6361 gtgcttgtaa ttgtttttgt aaacccaaca tgcagatttg gccacccaat atagttaacg
     6421 ctccctttgc aacaccggaa gacgaagatc cggctgcttg caagctcttg gttggatcgg
     6481 cctgagcctg tattatcacg ccaccccggt tgagataact caaggcggct tcccatattt
     6541 tatcggctgc accaacgcct tgcacgataa cccacataaa gaatacctgc atcatacaat
     6601 aaccagatgc cttgggtatt aacaaagcca agccgaaggt ggagcgaaga ggaatccaga
     6661 ttgaagacca tttttgcccg agcatttgcc cttcatgggc cgtgttcatg gtggaaacca
     6721 taagggtgta cattataatg ataccgccca gcgcaaggac agcggagtta aaaacaccaa
     6781 acatattgcc cattatctgg cttccagtgc cgtgcaatac accatcgact acaccaaata
     6841 ggttacctaa aaaaacaacg gacaaatcgc ttgccggagg agcgaaactc aaggctccat
     6901 tttcagccag tgcaagcgca ggaaaaaagc aaaggaggac cgtaatagct aatttattca
     6961 ttctttctca cccaataacc cttgcctgta ccattcttta acagtacatc ccaatttacg
     7021 ttgtttaatt tggtagtacc aaaaatgata acgaaaggct agtaccaatg cgatcataac
     7081 cacaaccaag cttattattg tagctttcca tgaaccatat aaaaattgat aacccgtata
     7141 aataagtatc ataaaagcag cgacaaccat aattatgctt agtctgaaca aggctttttg
     7201 tttgattacc aagtcagcct cactcaattt taatttcctg gctgcttcat caaatgattc
     7261 gatatgggtt ggttcctgag gtatgaataa ccgtttaatg ccattcacca aatacaaggt
     7321 gagtgacttc attctgtccc aatcgaacca tttacgaaca ttgataatcc ttgtaaaaac
     7381 tcttacgatc cttgatcctg atttcttttt catatttttt actttttcac aattttaagt
     7441 aatggacttt tatatatcat aatatactat agtattaagt agttgtttcg tctatcaacg
     7501 tatctcagta aaaaaagagt aaataatgcc tgatttaagc catgaagcct ccgcaaagta
     7561 ctggtttgag taccttgacc ccatgattta cagggttatc acttttatgg aaagtgttga
     7621 aaactggact cttgatggga atcctgaact ggaagaagca atgaaacagc tagggcaaga
     7681 acttgatgat attgaaaaaa ttgacttagg cttattagca gaagaagata aatttattcg
     7741 tatcgttggg aatattaaat caggaagagg cttacgtttg ctccaagcaa tagacacagt
     7801 ccatcctgga agcgcctccc gggtgttaat ccatgcggaa gaaaccagtt taagcagcag
     7861 tgacccggca ggtttttttc taaaacgcaa tattgtattt gaaagactaa ggttatta
//
LOCUS       DACTJE010000153         7855 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.153, whole genome shotgun sequence.
ACCESSION   DACTJE010000153 DACTJE010000000
VERSION     DACTJE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7855
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.153"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(361..1029)
                     /locus_tag="I9760_002453"
     CDS             complement(361..1029)
                     /locus_tag="I9760_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAT5044210.1"
                     /translation="MIDTIIFDFDGVILNSEPMHFEAIVQVLNQSGINLAYEEYMTHY
                     LGLSDISLFPKILNDKGLAFSSTEIHQVIERKVRVYNELIENSEQLPMTPDLDWFLVR
                     VARQYGKIGICSGSNRHSIIKILEKIHCGRLACYFKTIVSCEDVSLGKPSPEGYLLAA
                     HRLQSKPENCLVIEDSEHGVAAAKAGGMLVAGLLTTLSRDLLANADMIVHDFKELDHL
                     LNSH"
     gene            complement(1614..2057)
                     /locus_tag="I9760_002454"
     CDS             complement(1614..2057)
                     /locus_tag="I9760_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5044211.1"
                     /translation="MFFDDTYLDYKARVSYSLFCAKDFNETAAFIKANRILMTSTELA
                     AYMLKPENYKYAIIARIKNSMVGIVLFTDFESEVFINHLYVSPIHRFKGIGSNLLKEL
                     KAFMGQRTVSYIDTSNNYMPWMEVLDVSKKAAVINQLGSFYKCSK"
     gene            complement(2344..2750)
                     /locus_tag="I9760_002455"
                     /pseudo
     CDS             complement(2344..2750)
                     /locus_tag="I9760_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(2858..3100)
                     /locus_tag="I9760_002456"
     CDS             complement(2858..3100)
                     /locus_tag="I9760_002456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044212.1"
                     /translation="MLIIVVLGNFRESKKQDLTLILCSYVEKSEDSNATLPEPKETKG
                     HGRLPHTAYANANANEYNIPIETLSPGQPCRMDCGG"
     gene            complement(3257..3862)
                     /locus_tag="I9760_002457"
     CDS             complement(3257..3862)
                     /locus_tag="I9760_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAT5044213.1"
                     /translation="MSKLNKTRVYEVYDEIINWFDDARTKNLMESEYLNLIVNAIPPK
                     SSILDLGCGTGEPIAQFFIEKGFKLTGVDGSQKMIELCRKRFPDERWIVSDMRDINLQ
                     QQFDVILAWHSFFHLDHDSQRNMFKIFESHTKPGGVLAFTSGEEEGEVWSDNGGQQLY
                     HASLSTKEYESLLKNSSFKVLVHKVCDPECGEATVWVAQKI"
     gene            complement(3975..5780)
                     /locus_tag="I9760_002458"
     CDS             complement(3975..5780)
                     /locus_tag="I9760_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5044214.1"
                     /translation="MGIFLETKRLILKRVELSDLDLLVALRSDLEVMENTGYGGTQTI
                     EEVREYLDFAIPYQEKHGMGFCLVFEKESGNFVGEAGLFHLLFDDKQSEIELGYHLHK
                     KFWGKGYGTELAKALIQWGFQHLSVNKLISTAYPDNIASQKVLNKAGFDCMSKKQLPD
                     GEELFWYEIYKNDSVELVPYDSQWPKIAEVEIKKLRKILPTNHVIDIQHVGSTAIPGM
                     LAKPIIDIQITVDSLTAIKQTAIAVLKAYDYVYWAEDPDPEKMFFVKGMPPFGEKRTY
                     HVHIIEPKSKRGHDRIIFRDYLIAHPEVAREYEQLKIELAHEYTYDREQYTDAKTQFI
                     ASVLGKALVTDQIQNLTKYKGINELLHQLLLQIQSILANEFVGMYIGGSLASDSFNSE
                     TSDIDCYIITSKMLSENMIGKIEEMHNQFYSSKLKYAKKIEASYVPQKDLVDFDPQGM
                     RPYFNEGRYYLGHYGNNYLIELFVLREKGISIAGPDIKCLIQEISNQSLKAAIQKNLH
                     EYWESNLIDFSKFKRSDYQVFTILTMCRTLYSLETGKITSKIEAAQWAMQHLDTKWEK
                     LIELAAAWEPSQEINRLEETQNFVRYVLNKSREYQ"
     gene            complement(6095..7702)
                     /gene="murJ"
                     /locus_tag="I9760_002459"
     CDS             complement(6095..7702)
                     /gene="murJ"
                     /locus_tag="I9760_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAT5044215.1"
                     /translation="MSSDKTLNVKATGIIALAVMCSRVLGLIREVLFNALFGSASMGI
                     FLIAFRAPNLLRDLFAEGALSVSFITVFSKKIETEGEKSAWQLASKMLTLTSVFMSIL
                     CLFGIIFAKYLIFILAPGFSVKDAETTIFLTQLMFPFILLVSLAAIVMGMLNSKNVFG
                     MPALASSFFNIGSILGGALCGWFIDPSFGERALIGLAIGTVIGGLLQLGVQFPSLRKV
                     GFYFKPNFHWYDSGVRNTLILTIPAVIAASAVQINVLINSGFASYLGKEAVTWLNSAF
                     RLMQFPLGVLGVAIATITLPVVSRLAATNNHSQFGSTLARAIRLAVFLTMPAAVGLWF
                     FATPIISLIYEHGKFHASDSLQTAYALQFYALGLVAYSCIKVLSPAFYAIDKKWTPMI
                     VSFATIILNIILNYFLIFKLSMGHRGLALSTTVSATVNFLVLYVLMTRLHNLQNRYLF
                     NTILRCVLASAILGFMCQSIFAYGADFLYHPSFWIRAVSLAISISFAVLVYLSMCVIL
                     RVDEAKSIFMIIRHRLGFPIKFKSKQV"
BASE COUNT         2529 a         1526 c         1312 g         2488 t
ORIGIN      
        1 ggcaaatcaa tgtactttct catcaccatt ttgaaaaaaa atcaatcatt ctctatttta
       61 tcattaccga ccttttaacc tcccagatta aagagaaaat caacttgtgc tatggtttat
      121 tgcataaagt tcagggccag agccacacaa agatgaactt agaaatcttg ccatttttac
      181 tgatgtacta aaagaaccgt ataatgaatt ttgcccatca acattaaaaa attcggttta
      241 gttgtaaaac tgtgatttac ttgggagact gttggtttaa ttatcaagcg attttgtcgt
      301 aatacaatat cagttgaaga gttcaggatc tgaccctaaa tcagtgtaac ctgtctcttt
      361 ttagtgggag ttcaacaaat gatccagttc tttaaaatca tgcacaatca tatccgcatt
      421 ggccaacaga tccctgctca gtgttgtcag taaacccgca accagcattc ctcccgcttt
      481 cgcagctgct acaccatgtt ccgaatcctc tataaccaaa cagttttcag gcttggattg
      541 caaacgatgt gctgcaagca aataaccttc tggagatggt ttacccaagc ttacatcttc
      601 acaacttaca atggttttaa aatagcaagc caagcgaccg caatgaattt tttcaagaat
      661 ttttattatg gaatggcgat tggatccgct gcagataccg atttttccat attgtctagc
      721 cactcgaact aaaaaccaat ccagatcagg agtcataggc aattgctcac tgttctcgat
      781 taactcatta taaactctga ccttgcgttc tataacttga tgtatttcag ttgaggagaa
      841 tgcaagcccc ttatcattca gtattttggg aaacaaactg atatcactta acccaaggta
      901 atgagtcata tattcttcat aagcaagatt aatccctgat tgattcagta cttggactat
      961 cgcttcaaaa tgcatgggtt cactattaag tattacaccg tcaaaatcaa agattattgt
     1021 atctatcatg tattgcctct gtagattgac attgttaaac atatatattg tcaattaccc
     1081 aagagaaatt actttcatgc tggacgcttg ataaaaggga tatttcattt tattttagac
     1141 aactattctt aaaattgctt gtattggtct gcttaatcaa aagtggtata ggatttttta
     1201 aaccacctat ttcctgtttt tcaaaagtaa agagagctta aaagttgaca acaagcctca
     1261 taatgatttc atggcctttt actctttgct tatagtgtca tttggctaac tgccttggtt
     1321 ggtggtgtac gaatacctaa tgctttaatt gatgccggcg ctttttgctg ttgattcatg
     1381 tacgccaaaa tcttgtgaat cagctactgc tctttaatac acgcaatgat tcttacatga
     1441 gttaagtaaa gcatagaaaa cttacaaaag aaaacttcag tcgaggcaag aaatttagtt
     1501 tgaaattgaa atagccatcc aaaacattat gcaaatgaat ttacaaacaa ttaaatctct
     1561 tgatggtaaa gtcgaatatg cttttataag gggcagtaag attactgtgg ctgtcactta
     1621 ctacatttat aaaatgatcc taattgatta atgactgcag ctttcttgga aacatccaaa
     1681 acctccatcc aaggcatata gttgttactt gtatctatat aagagactgt tctttgtccc
     1741 ataaaagctt ttaattcttt caataaattt gagccgatcc ctttaaaacg atgtatagga
     1801 cttacataaa gatgattaat gaatacttcc gattcaaaat ccgtgaataa gacaatgccc
     1861 accatgctat tctttattct agcaataatg gcatatttat aattttctgg tttcagcata
     1921 taagcagcta actcggtgct agtcattaaa attcgattag ccttgataaa agcagctgtt
     1981 tcattaaaat ctttagcgca aaacaaagag taactaactc tggctttata atccaaataa
     2041 gtgtcatcaa aaaacataaa ttgctatatt agaggtctag atggcatata tttaaataca
     2101 taacccgcca aatgtacagc atgagtgatt caaaaaaccc ggatttcatc gtagttcatc
     2161 aggagctgcc cctaaatcac tattaaggga gttaatttct ttagagaaaa atagaatgtc
     2221 ttaatttatc tcacttcatt tttgacttat ttaagcctaa aaattttata atgtctgaca
     2281 aattgtatgc tttagtaagt tccattttgg aactcactac gtaccaagtt caataaatcc
     2341 tatttaaaac ttatcgagat ttaaaaaata acgagtcgct ttaagctcac cagttctttt
     2401 gagaatattt ttttaaccaa atcatttaaa tctctggttg ttgtagcaat cgttgcatta
     2461 gtaatgctca tataattttt agcacttaaa ccacccacaa ccccctcagg cccagcatga
     2521 aataatctta ataacacctt ttcttgcctt ggattaattt tcccttcggc ttcccttaaa
     2581 atgacagatt ttttaataat aaaatcaata tacatataat cctccagagc aatgttagca
     2641 aaccataaaa gccaggatgt caggtctaag gttttacttg ctaagcctaa ttgctgataa
     2701 tattcctttc tcttttttaa taatattttt gtcaatactg ttgagagcat atctggagca
     2761 gaccctgctt tttgacttct gactagaagt tggcaaaaaa caagacctga tcctgaacgc
     2821 tcatgttcac gataatacct ggggtgatgc gatagaacta ccctccacaa tccattcggc
     2881 aaggctgacc agggcttagt gtctctatgg ggatattgta ctcattcgca ttcgcattgg
     2941 cataagcagt atggggtaaa cgtccatgac ctttggtttc tttgggctca ggaagtgttg
     3001 cattagaatc ctctgatttt tctacatatg aacagaggat cagggtcaga tcttgctttt
     3061 tgctttctct aaaattccct aaaactacta tgataagcaa aaggcaagat ctgacccttt
     3121 agcgctgata cttgtattct gcaaactctt taataaattg caaaattaaa ggaacatcgg
     3181 acagtattgc aaatcttatt ttaaaattag ggttcatggt aattccactg ataattttgg
     3241 tttataacta aggattttaa atcttttgcg ctacccaaac tgtagcctcg ccacactcag
     3301 gatcacagac tttatgtacc agcactttaa atgatgaatt tttgagaagg ctttcatatt
     3361 cttttgtcga caaagatgca tgataaagtt gttggcctcc attatcactc cagacttcgc
     3421 cttcttcttc tccagaagta aatgctagta ctcccccagg tttggtatgg gattcaaata
     3481 ttttgaacat attacgttgg ctgtcatgat ccagatgaaa aaagctatgc catgcaagta
     3541 ttacatcgaa ttgttgttgt aaatttatat cgcgcatatc tgagactatc cagcgttcat
     3601 ctggaaaacg ctttctacaa agctcaatca ttttttgact gccatcaaca ccagtaagtt
     3661 taaagccttt ttcaataaaa aattgcgcta taggctctcc agttccacag cccaagtcca
     3721 aaattgagct cttcggagga attgcattca caattaaatt aagatattcc gattccatga
     3781 ggtttttagt gcgagcatca tcaaaccaat taatgatttc atcatatact tcatagactc
     3841 tagttttatt taacttactc atgctcaatt gctcctaaaa taacaatagc tggatttatt
     3901 gatgagtgag attttatcag actactacaa taaattttca gtcttcattt attctgaaaa
     3961 gtaatgtgat ctgctcattg atactcacga cttttattca gcacatacct gacaaaattc
     4021 tgcgtttcct ctaatctatt tatttcctgg cttggctccc aagcggcagc taattcaatc
     4081 aacttttccc atttagtatc aagatgctgc attgcccatt gtgctgcttc tattttagaa
     4141 gttattttgc ctgtttctaa agaataaagg gtgcggcaca tggttaaaat tgtaaacacc
     4201 tgatagtcac tgcgtttaaa ttttgaaaaa tcaattaagt tagactccca atattcgtgc
     4261 aaatttttct gtatagcagc cttcaaactt tgattggata tttcttggat taaacatttt
     4321 atatcgggcc ctgcaattga aattcctttt tctcttagta caaatagttc gattaaataa
     4381 ttattgccat aatgccccag gtaatagcga ccttcattaa aataaggacg catgccttgg
     4441 ggatcaaaat ctacgagatc tttttgagga acgtaggaag cttcaatttt tttcgcatat
     4501 tttaatttac ttgaataaaa ttgattgtgc atttcctcaa tctttccgat catattctca
     4561 gaaagcatct tgcttgtaat aatgtagcaa tcaatatcac ttgtttcact attgaatgaa
     4621 tcacttgcga gagaacctcc aatgtacata cctacaaatt cgtttgctag aatagattgt
     4681 atttgtaaga ggagctggtg tagtagttca tttattcctt tatattttgt caggttctga
     4741 atttgatcag ttactagagc tttgcctaat acactggcaa tgaattgtgt tttagcatcg
     4801 gtatactgct cccgatcata agtatattca tgtgccaatt ctatttttaa ttgttcatac
     4861 tcacgcgcta cctcaggatg agcaatcaaa taatccctaa aaatgattct atcgtgccca
     4921 cgctttgatt taggctcaat aatatgcaca tggtatgttc gtttttcacc aaatggaggc
     4981 atccctttta caaaaaacat tttttcagga tcaggatctt cggcccaata tacgtagtca
     5041 taggctttta atacggctat cgctgtctgt tttatagcgg ttagagaatc aacagtaatt
     5101 tgaatatcaa taataggctt agccagcatt cctggaatag ctgtgctacc aacatgttgg
     5161 atatcaataa catgattagt tggcaggatt tttcgtagct ttttaatttc cacttcagct
     5221 atctttggcc attgagaatc ataaggcact aattcaacgg aatcattctt gtaaatttca
     5281 taccaaaaca attcctcgcc atcaggcaat tgttttttac tcatgcaatc aaagcccgcc
     5341 ttgtttaaga ctttttgaga agcgatatta tctggatagg cggtagaaat gagcttgttg
     5401 acagataagt gctgaaaccc ccactgaatt agtgcctttg ctaattcagt gccatagcct
     5461 ttcccccaaa actttttatg caagtggtaa ccaagttcaa tttcggattg cttatcatca
     5521 aataacaaat ggaataaacc cgcttcgcca acaaagtttc cgctttcctt ttcaaatacc
     5581 agacaaaatc ccattccatg cttttcttga taaggaatag caaaatctaa gtactctctt
     5641 acctcttcta ttgtctgagt acccccataa ccagtatttt ccattacttc taaatcagat
     5701 ctcaatgcta ctaacagatc aagatcggat aattcgactc ttttaagtat tagtctttta
     5761 gtttctagaa aaatgcccat tgggtgtcta tctatatttt aaactggaag gaattatact
     5821 ttttatttga tgcttctacg atagcagttg ctacaaaagg tagcgccggg gtagcacgaa
     5881 aatattatat ttaattttaa atcagggata acttctggaa acaggatcag ggtcggatcc
     5941 tgaacgacct gtttatccta tatatatgat aatctagggg aagatacact tattaaatgg
     6001 tttgataaac caaagtcact ttgtgataaa agcaaacttt ctcatagact actgtagtct
     6061 ctcaataact tctctattat tcaattatta atgtttatac ctgttttgat ttgaacttaa
     6121 tagggaatcc taatcgatga cgaatgatca taaaaatact ctttgcctca tccactcgaa
     6181 ggattacaca catgctcaga tacactaaga cagcgaaaga aattgaaata gcaagggaaa
     6241 ctgcacgtat ccaaaatgaa ggatggtaaa ggaagtcagc tccatacgca aagatagatt
     6301 gacacataaa acctaatatt gcagatgcta aaacacaccg taatatagtg ttgaaaaggt
     6361 atctattctg caaattatgc aagcgggtca tgagaacata tagaaccaaa aaattaacag
     6421 tagcagaaac tgttgtggac aaagcgagtc cacgatgccc catactcaat ttgaaaatta
     6481 aaaaataatt caaaataata tttaagataa tggttgcaaa actgacaatc atgggagtcc
     6541 attttttatc aatggcgtaa aaggctggtg aaagcacctt gatgcatgag taagccacaa
     6601 gtccaagagc gtaaaattgc aatgcatatg cggtttgcaa ggaatccgac gcatgaaatt
     6661 ttccgtgttc ataaatgagg cttataatgg gggttgcaaa aaaccaaagt ccaactgcag
     6721 ctggcattgt caaaaacacc gctagacgga tcgcgcgagc cagtgttgat ccaaattgag
     6781 agtgattgtt cgttgcagca agacgagata cgactggtaa tgtaatcgta gcaatagcaa
     6841 cacctaacac tccaagtgga aattgcataa gtctaaatgc gctgttgagc catgttactg
     6901 cttcttttcc aaggtatgag gcaaatcctg aattgataag tacatttatt tgcactgcac
     6961 ttgcggcgat cactgctgga atagttaaaa tcaaagtgtt tcgaacccca gaatcatacc
     7021 aatgaaaatt gggtttaaaa taaaaaccaa cctttcttaa gctgggaaat tgtactccga
     7081 gttgtaaaag tccccctatt accgttccga ttgccaaacc aataagtgcc ctctctccaa
     7141 atgatggatc aataaaccat ccacataaag cgccaccaag aatagagcca atgttaaaaa
     7201 aactcgaggc taacgcaggc ataccaaaaa catttttaga attcagcatc cccattacta
     7261 tggcagcaag agacactaat aaaatgaaag gaaacatcaa ttgagtgagg aaaatcgttg
     7321 tttcagcatc cttcactgaa aaaccaggcg ctaaaataaa aatgaggtat ttggcaaaaa
     7381 taatcccaaa gagacataag atgctcatga aaacactggt caatgtaagc atttttgaag
     7441 ccagctgcca agctgatttt tctccttcag tttcaatttt tttagagaaa acagtaataa
     7501 aagaaacgga gagagcgccc tctgcaaaca aatccctcaa aaggtttggc gcacggaatg
     7561 cgataagaaa aatccccatg gaagcactgc caaataaagc attgaataag acttctcgaa
     7621 taagacctaa tactcgacta cacataacag caagggcaat gatccctgtt gcttttacat
     7681 ttagtgtctt atcactactc atggattatc caaagtctct tcaaaaacat taatatgata
     7741 gcccaagtat aagaggagcg ataccctatc atgcttggca tatgcaatag atattgtggt
     7801 gttaactcat tgaagtaggt attaaattgg aaacaattag gctctacccc aacaa
//
LOCUS       DACTJE010000154         7852 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.154, whole genome shotgun sequence.
ACCESSION   DACTJE010000154 DACTJE010000000
VERSION     DACTJE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.154"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            87..1190
                     /locus_tag="I9760_002460"
     CDS             87..1190
                     /locus_tag="I9760_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HAT5044216.1"
                     /translation="MHQELLKYLEMYHPQIAPFMHLQINEWDKNRPLEGVKILHHVPL
                     VPNTLLKIAALVAANAEVTVTNPASFCQAHPDAITCLHNSGIAYVENLDLLRDETFDV
                     YMDCGGQLHQRLGRPLTGAIELTGSGDQYYRSLKTLDFPVISIDHTMTKQLETVFGCA
                     ESSHQAIQKLTGVDPTNKNWLIFGFGKIGRGLAYFCLEHQVPVTVVDSSVHQCDLAFN
                     LGIEAIRPEEHDKLEKAVAKADIILTATGGKNIMSEYPRAWFDGKILGNLGLHDEFGP
                     NFTSQDVLYDKKPINFCLYDPTPMIFIDPEFYLHNLGCLLLLQGNFPRGMHDIPEDID
                     SSIIKDWCQYHRRSEKSIRCWFLTSQDFSLKTN"
     gene            complement(1312..2322)
                     /locus_tag="I9760_002461"
     CDS             complement(1312..2322)
                     /locus_tag="I9760_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5044217.1"
                     /translation="MSTDNLLQPKLIEATLGDYPVIQNMARFYVYDMSRYCGFISEEW
                     TCPPDGLYESYDFKIYFTDPERKAFLIQIDNELAGFALLNKFGIQAVDWSMGEFFILA
                     KFQGKGIGQSVAHQLWRTHPGEWEISVIPENIPALSFWRQAVMTYTLGRYKEQIKTVN
                     YDIHQTQRIILSFNTQKQNKSCHSIPKKPITIDFVDNIPKSLEKKMTEDLIAYERTHG
                     IDVNYKRFSIVLSEEGVTCGVINAFTAFAEIYIDDIWVDKAYRGKGYGKKLLQTLENH
                     FKGQGFNNINLCTSAFQAPEFYRKCGYTAEFTRINQANPKLSKTFFVKFFDDENQTQG
                     LF"
     gene            complement(2570..3184)
                     /locus_tag="I9760_002462"
     CDS             complement(2570..3184)
                     /locus_tag="I9760_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044218.1"
                     /translation="MVWDATTTNAISNAAHALFLLLYLIGACIHYLKKDHTFSLLIVF
                     FFLNLLVLKVLGVYVHYYPSHLHLPPAWIAISLLVIMLNYLLVQSMQMPDLCRVIVVF
                     LSIVFTYLFLTHDGNYTYIALPVILVCLIAAYYSQAKVRIGFVMVVISNLIWIVTRHI
                     ANYLTGHEIPIEYRYDNDIYHILLILSTYVIYKGIAEGQWKHPR"
     gene            complement(3343..3717)
                     /locus_tag="I9760_002463"
     CDS             complement(3343..3717)
                     /locus_tag="I9760_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug:proton antiporter"
                     /protein_id="HAT5044219.1"
                     /translation="MLSDPNLVLFYVKNPAKSEEFYKNLLDTQPIESSPTFAMFVMKT
                     GLRLGLWAQEEIEPKAHQTGGGMELSFQVNSNEMVDEIHRQWSDKEISIIQPPTQMDF
                     GYTFVGVDPDEHRLRIFCLKRT"
     gene            3907..4680
                     /locus_tag="I9760_002464"
     CDS             3907..4680
                     /locus_tag="I9760_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5044220.1"
                     /translation="MNSRIETFHQMEWHFFSLISMELLSLEGLKACNSGMPIPALNPV
                     FYSGKSTLLVAEIYQCKRFYESHSVPWALMVPDYLSDTKTDNVLAQSGFLSIDQGVAM
                     YLSIQDLGMPGFKSDLLIKRMDNEMETWILPTIPAFESTAEIAKIYRVRHQEAIEKSS
                     DIYHFSGFIQEQIVCSLTLTVMGDCARIDDVATFPKYQKNGYATQLILSELQILKDTN
                     ISWCFLEASADGLNIYKKIGFRELFKNLYYEEQYHYEQN"
     gene            4667..5353
                     /locus_tag="I9760_002465"
     CDS             4667..5353
                     /locus_tag="I9760_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HAT5044221.1"
                     /translation="MSKTERLFHLLQILRRYRYPLSGQFLANELGVSTRTIYRDIAAL
                     QAQGADIEGEPGLGYVLKPGFMLPPLMFNEEEIEALVLGSRWVAKKTDTHLQQAAHNA
                     LAKIASVLPADLRQQLELSSLLIGPGEVISGEDESLALVRKAIRKELKLFIQYHDLKG
                     NVSTRTIWPIGLGYFDQVRVLIAWCELKKDFRHFRADRIVDVENLGVCYAKRRSVLLH
                     DWRKQNNIPG"
     gene            complement(5513..6772)
                     /gene="lem19"
                     /locus_tag="I9760_002466"
     CDS             complement(5513..6772)
                     /gene="lem19"
                     /locus_tag="I9760_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem19"
                     /protein_id="HAT5044222.1"
                     /translation="MIDMISITTYWQQFKDCIDQLRSSVDNKEYSNQIATADLMIEIG
                     LTIEQLGITQIQQVGDRLHEKAKLFGYRVPFVTPEKKYEFCLPKITNAETLIFAIVYY
                     QHLKNYENVEYQGSINWFRSKVIHGVNEKKISTRAQAEAMLGEIRRYCQSQNLAFNES
                     EFIKKNDESAKEYTTRLFKNFYANKTAKSEPIGLKQLNESKESNLSDELAKLEKSLED
                     SRNKRNQLIVKIDALAKKLADYHLLSQNQHKLNNEWNNKWFFTKFFDWIMSWFSNDSF
                     IKNLKTSYEQVINAEHELKQEFHPFDTADAYRAELKKQLEETHIECDKNQKQINEYQL
                     KQLEHKLEQRLEQKLQEKLQQDTAVEQKEPSVTKVLSEIKHETNGSDFSSNLNHYYGF
                     FKEHLPNRETLGAIAVGVAAIALQNLM"
     gene            complement(6907..7419)
                     /locus_tag="I9760_002467"
     CDS             complement(6907..7419)
                     /locus_tag="I9760_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044223.1"
                     /translation="MSVINGKKIITIILGIACLFCSIHPVNSAPDFSNKPHVREKNKN
                     LLGVFVGGNHAQNDEAFTYGFEYHRVLSMPFGFSAVIEHTPVNVEHNNQVELIGLGTL
                     NVFRNLTFGIGPGIRYEKDEPNRMVGRLGIGYIIHFPPDIEITPNIDLDFIEGGEKEL
                     IFGITFGKQF"
     gene            7657..>7852
                     /locus_tag="I9760_002468"
     CDS             7657..>7852
                     /locus_tag="I9760_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044224.1"
                     /translation="MYFSRCISHGAIAGIIAGIVFTFFLVMGGMLETLGSMINMPTKG
                     GGLLVHAVMSIGSGIVFALVL"
BASE COUNT         2347 a         1337 c         1538 g         2630 t
ORIGIN      
        1 taaaatatgc tttattaatt cgtgttattg ttaatgagtt ttattatagt attgtcattc
       61 tttctcatac tcatatggtc taaataatgc accaggagtt attaaagtac ttagaaatgt
      121 atcacccgca aattgcgcca tttatgcatc ttcagattaa tgaatgggac aaaaacaggc
      181 cactggaagg agtgaaaata ctgcatcatg tgcctttggt tcccaataca ctgctaaaaa
      241 tagcagctct tgtcgctgca aatgctgagg tgaccgtaac caatccagcc tctttttgtc
      301 aggcacatcc tgatgcgatt acttgtcttc ataattcagg aattgcttat gttgaaaatc
      361 ttgacctttt aagagatgaa acgttcgatg tttatatgga ttgtggcggg cagcttcatc
      421 agcgtctcgg aagacctttg acgggagcta tagagttaac aggctcaggg gatcagtatt
      481 atcgtagtct gaaaacactt gattttccgg ttataagcat tgatcacaca atgacgaaac
      541 aattggaaac agtatttggc tgtgctgaaa gttctcacca agcgattcaa aaactcactg
      601 gtgtcgatcc cacaaacaag aattggctaa tctttgggtt tggtaaaata gggcgagggc
      661 tcgcttattt ttgtctggaa catcaagtac cagttactgt agtggatagt tcagtccatc
      721 aatgtgattt agctttcaat cttggtatag aggctattag acccgaggag catgataagc
      781 tcgaaaaagc tgtggctaaa gcagatatta tattaactgc aacgggcggt aaaaatatca
      841 tgagcgaata tcccagagcc tggtttgatg gcaaaatttt ggggaattta ggactgcatg
      901 atgagtttgg tcccaatttc acctctcagg atgtattata cgataaaaaa cctattaatt
      961 tttgcctata tgatcctacg ccaatgattt ttatagatcc tgagttttat ttgcataatc
     1021 tgggatgttt attgctttta cagggaaatt ttcctcgagg aatgcatgat attcctgaag
     1081 atatagattc ctctattatt aaagactggt gccaatatca taggcgttct gaaaaaagta
     1141 ttcgatgttg gtttctgact tcccaggatt tttctcttaa aacaaattaa tataaacagt
     1201 tcgaataaga cggtttatag gcactttaat tagtctattt aattgaaaat tactttttca
     1261 ggacttctga atatcaataa gatgtgggga gttatttaga agttctattt ttcaaaatag
     1321 tccttgtgtt tgattttcat catcaaaaaa tttcacaaag aaggttttgg aaagcttggg
     1381 attggcttga ttgatgcgag taaattcagc cgtgtatccg cattttctgt aaaattctgg
     1441 tgcttgaaag gcgctggtac ataaattgat attattaaat ccttgccctt taaaatgatt
     1501 ctcaagagtt tgcagtaatt ttttgccata tcctttacca cgatatgctt tatctaccca
     1561 tatgtcatcg atgtatattt ctgcaaaagc agtaaaggca ttaataacgc cacaagtcac
     1621 cccttcttca gatagtacta tcgaaaaacg cttgtagttc acatcaatac catgagttct
     1681 ttcataggct atcagatctt ctgtcatctt tttttccaga gatttgggga tattatccac
     1741 gaaatctatt gtaataggtt tttttggaat ggaatgacaa gatttgttct gtttttgtgt
     1801 attaaaactt aaaataatgc gttgggtttg gtggatatca taattcactg tctttatctg
     1861 ttctttgtac cgtccaagag tataggtcat tactgcctgg cgccaaaacg ataaagccgg
     1921 tatgttttcc ggaatgaccg atatttccca ttcacctgga tgagtgcgcc atagttgatg
     1981 agctactgat tggccaatgc ctttcccttg gaatttagcc aggatgaaaa actctcccat
     2041 actccaatca acagcttgga ttccgaattt atttaataaa gcaaaaccgg ccagttcatt
     2101 gtcaatttga ataaggaagg cttttcgttc ggggtcggta aaataaattt tgaaatcata
     2161 actttcataa agtccatctg gtgggcatgt ccattcttcc gaaataaaac cgcaataacg
     2221 tgacatatca taaacataaa atcgcgccat attttgaata actggatagt caccaagtgt
     2281 tgcctcaatt aacttgggct gtaataaatt atcagtgctc atgaatattt attctcaaga
     2341 gattttctta attatttgga taaggtatca aagtgttctg tggttttata gagtgattgt
     2401 tgttaatcgc ataattaatc agctattttg gtaggctgtc tagaataact aaaagaattc
     2461 catgaatgta tgaattcatg ctcaggaaat ggttcatttc aaagtgatta aattattcac
     2521 tcatatcttg acattgcttt tgataagtat aggtttctca atgatatttt taacgaggat
     2581 gtttccattg cccttcggca atacccttat agatcacata agtagagaga attaaaagga
     2641 tatgatagat atcattatca tagcgatatt ctataggtat ttcatgacca gtgagatagt
     2701 ttgcaatatg tcttgtaact atccatatca ggtttgatat aacgaccatg acaaagccaa
     2761 tcctgacttt tgcttgcgaa taataagccg caatcaaaca tacaaggatg acaggaaggg
     2821 caatgtaggt ataatttcca tcgtgggtca ggaacagata agtgaagacg atggatagaa
     2881 atactacgat aactcggcac aaatcgggca tttgcattga ttggacaagc agataattaa
     2941 gcatgattac aagcaaacta atcgcaatcc aggctggtgg taaatgcaaa tgagaagggt
     3001 agtaatggac ataaactccc aataccttga gaacaaggag gtttaagaaa aaaaacacaa
     3061 tcaaaagcga aaaagtatga tcttttttta agtagtggat gcaggcacca attaagtaca
     3121 ataacaggaa aagcgcatgg gcggcattag agatggcgtt tgttgtcgtt gcgtcccata
     3181 ccattctgta tctccttttt aaaaaataca gctatcagcg gtcgatgtcc cgatgtttgt
     3241 tcatttgcta atagtaattg tagcttgtag cagcttggga tgtgtgatat cactcactta
     3301 aaagccatac tacaagctat gattggtcat tactccaatt gcttacgttc gttttagaca
     3361 aaaaatacga agacgatgct catcgggatc aacaccaaca aatgtgtatc caaaatccat
     3421 ttgtgtggga ggttgaataa tagaaatttc tttatcagac cattgcctgt gtatctcatc
     3481 gaccatttca ttactgttaa cctgaaaaga cagctccata ccccctcctg tttgatgagc
     3541 tttaggctca atctcttctt gcgcccataa tccaagacga agccctgttt tcatgacaaa
     3601 catagcgaat gtcggtgaag attcaatagg ttgagtgtct aacagatttt tataaaattc
     3661 ttcacttttt gccgggtttt tgacgtaaaa taaaaccaaa ttagggtcac ttaacatgtt
     3721 attttcctta ttttttatta atcatcgcca gtataaacaa ggctaatgac agattttgtc
     3781 agtagtgttt taagtgccta atattgacct tcttgataat aaaaaacaca tgaaacagga
     3841 ttttggtgtt attatgaaaa caaaatttag cctatcctgg ctcaaaaatt ttgatgagtt
     3901 tattttatga acagccgtat tgaaactttc catcaaatgg agtggcattt tttttcactg
     3961 attagtatgg agcttttatc attggaagga ttgaaagctt gtaacagtgg aatgccaatt
     4021 cctgctctaa atcccgtgtt ttattcaggt aaatcgactc tgcttgtggc agagatttat
     4081 caatgcaaac gcttttatga gtctcattca gtaccttggg cactgatggt tcccgactat
     4141 ttatctgata caaaaacaga taatgtactt gctcaatcag gatttttatc tattgatcaa
     4201 ggagtggcca tgtacttgtc aattcaggat cttggaatgc ccgggtttaa atctgacttg
     4261 ttaataaagc gcatggataa tgagatggaa acctggattt tacctactat ccctgcattt
     4321 gaatcaactg cagagattgc aaaaatttac agagtaagac atcaggaggc tattgagaag
     4381 tcttctgata tttatcattt ttccggtttt attcaagagc aaattgtctg ctcattaacg
     4441 ctgactgtta tgggagactg tgcaaggatt gatgatgtag ctacttttcc caaatatcag
     4501 aaaaatgggt atgcaacaca attgatttta tcagaacttc aaattctgaa agacactaat
     4561 atttcctggt gttttttaga agcatctgca gatggtttga atatttataa aaagattggt
     4621 tttcgtgaac tgttcaaaaa tctttattat gaagagcaat accactatga gcaaaactga
     4681 gagactcttt catctgttgc aaattttgcg gcgttatcgt tatcccctta gtggacaatt
     4741 tcttgccaat gagctgggtg tcagtacccg taccatctat cgggacattg cggctttaca
     4801 ggctcaaggt gctgatattg agggtgaacc aggattgggt tatgtgttaa aaccaggctt
     4861 tatgttaccg cctttgatgt ttaatgaaga ggaaattgag gctcttgttt taggctccag
     4921 atgggtagct aaaaaaacgg atactcactt gcaacaggct gctcataatg ccttggctaa
     4981 aatcgcttct gtacttcctg ccgatttgcg acagcaattg gagctatcca gtttattaat
     5041 agggcctggt gaagtgattt caggagaaga tgaatctctc gcattagtca gaaaggcaat
     5101 acgaaaagaa ctgaagttgt ttattcaata ccatgactta aaaggaaatg tttcaacgcg
     5161 tacgatttgg ccaattggac ttgggtattt tgatcaagta agagttttga ttgcctggtg
     5221 tgaattaaaa aaggattttc gtcattttag agctgataga attgtagatg ttgaaaactt
     5281 gggggtctgt tatgcaaagc gacgctcggt gttacttcat gattggcgaa agcaaaataa
     5341 tatcccaggc tgatgacaac acgatgttat tgacgtgtta acttgaacag gtaaaattta
     5401 atgccgggaa tcaagtttta ctttcttccc ggattaagat tgcttccaat gccaaagacg
     5461 tgctgaactc ccacttattc ctgagtgaga gaacctcgta tttcaaacgg ttttacatca
     5521 aattttgaag ggcaatggct gcgacaccaa cagcaattgc tcccagcgtt tctcgatttg
     5581 gcaagtgctc tttaaagaaa ccataataat gatttaaatt tgaagagaaa tcactgccat
     5641 tggtttcgtg tttgatttct gatagcactt ttgtaacgga aggttctttt tgttcaacag
     5701 cagtgtcctg ttgtagtttt tcttgcagct tttgttctaa cctttgttca agtttgtgtt
     5761 ctaactgttt caattgatat tcgttaattt gcttttgatt cttatcgcat tcaatgtgag
     5821 tttcttccag ttgttttttt aattcagctc gataggcatc cgcggtgtcg aaggggtgaa
     5881 attcttgctt cagctcatgt tcagcattaa tgacttgctc atagctggtt tttaaatttt
     5941 tgataaatga atcattagag aaccaggaca tgatccagtc gaaaaatttt gtaaaaaacc
     6001 atttgttatt ccattcattg tttaatttat gctggttttg cgaaaggaga tgatagtctg
     6061 ccagtttctt tgcaagagca tctattttga caatcaattg atttctttta tttcttgaat
     6121 cttcaaggga tttttcaagc ttggctagct catcggacag gttggattct tttgattcgt
     6181 taagttgttt tagcccgatt ggttcacttt ttgcagtttt attggcataa aaattcttga
     6241 acaatcttgt tgtatactct tttgctgact cgtcattttt ctttatgaat tcagattcat
     6301 taaaggcaag attttgactt tgacaatatc gtctaatttc tcctaacatc gcttcggctt
     6361 gagcacgggt cgaaattttc ttttcattaa caccatggat tacctttgat ctaaaccaat
     6421 tgatagaacc ttggtattcg acattttcat agttcttaag gtgctgatag taaacgattg
     6481 caaaaatcag ggtttcagcg ttagttattt tgggtaaaca aaattcatat tttttttcag
     6541 gggtaacaaa tgggactcga tatccgaaca atttggcttt ttcgtgcagt cgatctccta
     6601 cctgttggat ttgagtaatc cccagttgtt ctattgtcaa gccaatctcg atcataagat
     6661 ccgcggtggc tatttgattg gagtattctt tgttgtctac agatgaacga agttgatcaa
     6721 tgcaatcttt aaattgttgc caataagttg ttattgaaat catatcaatc attatttaag
     6781 aaaaaagagt cataaattta caccggtctt ctaaaaaaag ctattattta gtcgtgatta
     6841 ttaatcagaa aatttttaaa aagagagaaa gcgggagaga caattcatat aggggaaaag
     6901 aactatttaa aattgtttgc caaaagtgat accaaagatc agctcttttt cacccccttc
     6961 aataaaatca aggtctatat tcggggtgat ttctatatcc ggaggaaaat gtatgatata
     7021 tccaatcccc aatcgaccaa ccatgcggtt gggttcatct ttttcatatc taattccagg
     7081 gcctatgcca aaagtcaggt ttctgaatac gtttaatgta ccaagaccta tcagttctac
     7141 ttgattatta tgttccacat taacgggggt atgttcaata accgcactaa aaccaaaagg
     7201 catggaaagg actcggtggt actcaaatcc ataggtaaac gcttcatcgt tttgagcatg
     7261 atttccacct acaaaaacac caaggagatt tttgtttttt tccctcacgt gcggtttatt
     7321 tgagaaatcc ggagcagaat ttaccgggtg tattgaacaa aaaaggcatg caatccccag
     7381 aatgatagtt attattttct taccatttat taccgacatg agttctcaag acagggttat
     7441 ctatttcttt attaaatcgc aaaatgacta atatttaaag aacatttgaa tcgatatgtt
     7501 atataaaagt ctttattaac aataagatgt tgataatgtg gctggcaaaa aattttttaa
     7561 gacattgcca ttaatttttc taccttaatt gttgattatt aagtaaactg atgattttga
     7621 atggtaagct gggatctatt taataaggag attggcatgt atttttcaag atgtatttca
     7681 catggcgcta ttgcaggaat tatagcagga attgttttta cctttttttt agttatgggg
     7741 ggcatgctcg aaacattggg aagtatgatt aatatgccaa caaaaggagg aggccttttg
     7801 gtgcatgctg taatgagcat tggcagcggg atcgtttttg ccctggttct gg
//
LOCUS       DACTJE010000155         7839 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.155, whole genome shotgun sequence.
ACCESSION   DACTJE010000155 DACTJE010000000
VERSION     DACTJE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.155"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1280
                     /locus_tag="I9760_002469"
     CDS             <1..1280
                     /locus_tag="I9760_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAT5044225.1"
                     /translation="ASGFIASQFVTDLLIAGHEIICCVRNTKHTQRIFPGAQVIFCDF
                     INDTKPEIWSKRLQGIDVVINCVGILYHPDERIIWNVHYETPKALFDACINSGVKKII
                     QISALGIDKVDVSYATSKKAIDDYLLTLSIPSVIVRPSYVYGKGSYGGSSLFRGIAGT
                     PFFTAIPGQGTQKFQPISLNDLSQAIVRLVSTPVTETIILHAVSKKIITLEEIIIKLR
                     SWLGFTKTRIIHIPLTFIRLLTRLGDLLPYSTINTVSYKMLASDNITTQEETQRFANY
                     VNFTPRDYEEGLYSQPSSVQDHWHAQLYFLKLPLRLGISFVWIWTALCCLFFYPKSDS
                     LTLFAQIGFSSLWQLFLFYASALWDGLLGIAMLFNYRFKLTSILQLITILVYTVIITI
                     KLPYLWLDPFAPIAKNIPLLIAILIGLGLESDK"
     gene            1280..1747
                     /locus_tag="I9760_002470"
     CDS             1280..1747
                     /locus_tag="I9760_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HAT5044226.1"
                     /translation="MLYFYLKFIHVLSSTILFGTGIGTASVMIYGHRTKNPIVIAAIS
                     KYVVFADWIFTGTSGILQPLTGFAMIYLAGFSWTSLWILGSILGYVVAACCWFPVVCL
                     QIKMRDLAIETVKNNTTLPEKYHRYFLYWFILGWPAFLSLIGVFYLMTNKPVF"
     gene            complement(1801..4242)
                     /locus_tag="I9760_002471"
     CDS             complement(1801..4242)
                     /locus_tag="I9760_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein FimV"
                     /protein_id="HAT5044227.1"
                     /translation="MKKTVLHAAIFSLTMPISLYALGLGEMKVQSSLDQPFSAEIELI
                     DVGADPLASIKVSLADPEQFEQIGLERTAVLSLLRFQVERNPKGTPVVKVSSTERMTE
                     PYMEIVVDLTWPKGQLYKAYTVLLDPPGYQLGITTAQSRPVYYKKKSASYSNEPGVIE
                     KTVITTVEHGPASRSDRKKKTTYGPTVTNENVWQIAQRYKTSKLILPQVVLAIVGANP
                     EAFKDGNLNGLKVGVRLVIPSTEEIAKVPSDLAIEEVMAHDKAWNEHTSINHVLAPPY
                     INSSIVDVTPKQTKASSIPVIPKFTIGENSNIPTLQQLVPDSTSVSIDTSTQQQPVQN
                     NNIVQTTEQNEKIKAEISITTAAVESVREANALLMEQLRILQEQNKKLQEKLDKREKE
                     IELMRTQVQTMLKERKAIASQVSSLPNNEQGMNLWPYIILLIFAAGGAGFVYWYLRQR
                     QHREDISPYKTDSDSSPKPFMPLVEPSKYEPDTQADDKETSVQEKVSDSEKETGVGVS
                     KADWTEYQPKEMDKIEKADAVESETVEDVTYLGSTEQLRAKEYTELESEVQTKPEAQA
                     EPEVQAGENQIMFEPVMEPEQFDVNRDDEVDNSQLSEDKTQQTEGHQSSEMIEFESGL
                     HELIKDKPKTKITESTDEGDDNGIDFIPLTINEADKTLKHEQEIEKSDKKEDKKSEKF
                     STKENKLDLDNSDFDMKNLESEPEISQEEKEAHISEITEFDQKSTQEEKTEKGISTSQ
                     SVDKATFTKSSGENNETSSPLKSKKALDTLLDLAKTYISMGDIETARSSLEEVIEHGS
                     ANQKEQARQLLEQLK"
     gene            4511..4711
                     /locus_tag="I9760_002472"
     CDS             4511..4711
                     /locus_tag="I9760_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044228.1"
                     /translation="MNRENEVIEIFLMDISKKEKCKLLQDFLLDCKNEMEAQDQNMHP
                     EVHHNLSQAYQLAQNYLRKLQE"
     gene            4798..5652
                     /gene="folD"
                     /locus_tag="I9760_002473"
     CDS             4798..5652
                     /gene="folD"
                     /locus_tag="I9760_002473"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAT5044229.1"
                     /translation="MPASLIDGREISALRRAELKQRVLYHVEQGQRAPGLAVVLIGND
                     PASVIYVSNKRKACEEVGITSHSYDLPAETTQEKLIELINELNQSDKIDGILIQLPLP
                     KHINERTIIEYIKPEKDVDGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRK
                     HAVVIGASNIVGRPMSLELLLAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIAT
                     DWLREHQVVIDVGMHRLPDGSIRGDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTM
                     MSAARLRE"
     gene            complement(5655..5864)
                     /locus_tag="I9760_002474"
     CDS             complement(5655..5864)
                     /locus_tag="I9760_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044230.1"
                     /translation="MGLHEEFEDQSFSDYNYDEEIDDHIDDLNQKKKIRRMLEDRLER
                     KRLKEEFKDDFDELSGEFDWDELDK"
     gene            6140..6988
                     /locus_tag="I9760_002475"
     CDS             6140..6988
                     /locus_tag="I9760_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAT5044231.1"
                     /translation="MLSYQHGYHAGNFADVIKHITLTRLLAYLTHKDKPLFYLETHSG
                     RGIYDLKDKQSLKTEEYKEGINPVWLDRENLPSLFLEYISVIKQINLNSTLSYYPGSP
                     YFAINQLRSQDRLYLCELHPTEYNFLLKLPHFNKKVYVNHTDGVSKLNALLPPPEKRG
                     LIFIDPSYERKEEYKEIPYAIKNAYSKFSTGLYCVWYPVVNKAWTEQFLRKMREISSK
                     SVRIELHLNPLINEGMTGCGLWIINPPYTFPSEIKLVLETLTTYFNPGSSSYMIESGS
                     KLCHEL"
     gene            6992..7756
                     /gene="gloB"
                     /locus_tag="I9760_002476"
     CDS             6992..7756
                     /gene="gloB"
                     /locus_tag="I9760_002476"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAT5044232.1"
                     /translation="MTILPISAFSDNYIWTFIDKIAGVLDCVDPGEAAPIIRFAQSNQ
                     LTLRTILLTHHHYDHIGGVDSLIKQWPSCKVYGPIDERINNVTHPIKQGQSVQVGSLH
                     FHILFNPGHTSTHISYYEPQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPR
                     NTKVFCAHEYTFQNLKFAHTVEPCNSSVINYMQQILKQPSSCTLPSNIDLELSINPFL
                     RTDKEQVKQYALSHGANSSDSLDVFKVLRNQKNSFK"
BASE COUNT         2342 a         1502 c         1374 g         2621 t
ORIGIN      
        1 gcgcatctgg gtttatcgct tcacagtttg ttaccgatct gctgattgct ggacatgaaa
       61 tcatttgttg tgtacggaat accaaacaca cgcaaaggat ttttcctggg gcacaagtta
      121 tcttttgtga ttttatcaat gacacaaaac ctgaaatctg gtcaaaaaga ttgcagggga
      181 ttgatgtggt tattaattgt gtagggattt tataccatcc tgatgagaga atcatatgga
      241 atgtccatta tgaaactcca aaagcacttt ttgatgcgtg tataaactct ggggtaaaaa
      301 aaattattca aatatctgct ttgggaattg ataaagttga tgtgagttat gctacgagta
      361 aaaaagcgat agatgattac ttactgacac tctctatccc gtcagttata gtaaggccct
      421 cttatgtgta tggaaaaggc tcttatggag ggtcttctct attcagaggg attgctggaa
      481 cacctttttt taccgctata cctgggcaag ggacacaaaa atttcaacct atctctctaa
      541 atgacctatc tcaagcaata gtacgattgg tgagtacgcc tgtaaccgaa actattattt
      601 tgcatgccgt aagcaaaaaa ataatcacgc tagaagaaat aataataaaa ctgcgttcct
      661 ggcttgggtt caccaaaacc agaataatac atattccatt gacatttatt cgactattga
      721 cccgacttgg cgatttgctc ccctattcaa ctataaatac tgtttcgtat aaaatgcttg
      781 ccagtgataa cattactact caggaagaaa cacagcgatt tgctaattat gttaacttta
      841 ctccaagaga ctatgaagaa ggtctctata gtcaaccgag ttcagtccaa gaccattggc
      901 atgcgcaatt atactttctt aaacttcccc taagactcgg aatatccttt gtctggattt
      961 ggacagcctt atgttgtttg tttttttacc ctaaaagtga ctcattaact ttgtttgctc
     1021 aaataggttt ttcatcctta tggcagttat tcttatttta tgcttcagct ttatgggatg
     1081 gccttttagg tatagccatg ttgtttaatt atcgatttaa attgaccagt atattgcaac
     1141 tgattaccat tttggtatat acagtgatta ttaccatcaa actgccttac ctttggttag
     1201 atccatttgc tcccatagcc aagaacattc ctttgttaat agctatttta ataggtctgg
     1261 gtttggagtc tgataaataa tgctctattt ctatttaaag tttatccacg tgctgagttc
     1321 aacgatactt tttggtacag gtataggaac agcaagtgtg atgatttatg gccatagaac
     1381 aaaaaatccg attgttattg ctgccatttc aaaatatgtg gtttttgctg attggatatt
     1441 cactggtaca tctggaatat tacaaccact cacagggttt gccatgattt accttgctgg
     1501 attttcttgg acttctttat ggatactggg atcaattctt ggatatgtcg ttgcggcatg
     1561 ttgttggttt cctgtcgtct gcctgcaaat caaaatgagg gatctagcca tagagaccgt
     1621 aaaaaataac actactttac cagagaaata tcaccgatat tttctttatt ggtttattct
     1681 gggttggccg gcattcttaa gccttattgg agtattttat ttgatgacta ataagcctgt
     1741 cttttaaata gcaaagacga atattaagag attgggtggt cgtaattttt gtgttatttg
     1801 ttactttaat tgttccagta actgcctggc ttgctctttt tgattggcac ttccatgctc
     1861 gatcacttcc tcaagagaac ttcttgctgt ttctatatcg cccatactaa tataggtttt
     1921 tgccaaatct aacaaagtat ctaatgcctt cttactcttt aatggactgg aggtttcatt
     1981 gttttcaccg gatgacttgg tgaatgtcgc cttatcaaca gattgagatg tagaaatacc
     2041 tttttctgtt ttttcttcct gagtagattt ctggtcaaat tcagttattt ctgaaatgtg
     2101 ggcttctttt tcctcctggc ttatttcagg ttcggattcg aggtttttca tatcaaagtc
     2161 tgaattatct aaatctaact tattttcctt tgtagaaaat ttttctgatt ttttatcttc
     2221 ctttttgtct gacttctcga tttcttgttc atgctttaat gttttgtcag cctcatttat
     2281 agttaaagga atgaaatcta ttccattatc atctccttca tctgttgatt ctgttatttt
     2341 agttttgggc ttatctttga ttaattcatg taaacccgac tcaaactcta tcatctcaga
     2401 actttgatgg ccttcagttt gctgagtttt atcttcactt aactgagaat tatcaacctc
     2461 atcatccctg ttaacatcga attgctcagg ttccataacg ggctcaaaca taatttgatt
     2521 ttctccagcc tgaacttctg gctcagcctg ggcctctggt ttagtctgaa cttctgattc
     2581 taattctgta tattcttttg ctcttaactg ttctgtactt ccaagatatg tcacatcctc
     2641 aactgtttca gattctactg catcagcttt ttcgatttta tccatttctt taggttgata
     2701 ctcagtccaa tccgctttag aaacaccaac tccagtttct ttttcactgt cagatacttt
     2761 ttcctggacg gaagtttcct tatcatcagc ctgggtatct ggttcgtatt tcgatggctc
     2821 aactaaaggc ataaatggct tcggacttga atcagaatct gttttgtaag gagaaatatc
     2881 ctctctgtgt tggcgttgtc tgaggtacca ataaacaaat cctgctcctc ctgcagcaaa
     2941 tatcaataaa atgatatacg gccaaaggtt catcccttgt tcattattgg gaagtgaact
     3001 gacttgagac gcgatggctt tacgctcctt caacatggtt tgaacctgcg tacgcattaa
     3061 ctctatttct ttctcacgct tatccagctt ttcctgcaat tttttatttt gctcttgcag
     3121 aattcgtaat tgttccatca ataatgcatt tgcttcccga actgattcaa ctgctgctgt
     3181 tgtaattgaa atttctgcct taattttttc attttgttct gttgtctgta ctatattgtt
     3241 gttttgtaca ggttgctgtt gggtagatgt atctatcgaa actgaagtgc tatcggggac
     3301 taattgttga agtgtcggaa tgttagagtt ttctccaata gtaaactttg gaattaccgg
     3361 gattgaagaa gctttagtct gttttggagt cacatccact attgaactat tgatgtatgg
     3421 cggagctaat acatgattaa tagatgtatg ttcattccag gctttgtcgt gagccatcac
     3481 ctcttcaata gccaaatcag aaggtacttt tgcaatttct tcagttgatg gaataactag
     3541 ccgaacgcca acttttaaac cgtttaaatt tccatcctta aatgcttcgg gattcgcgcc
     3601 aactattgct aatacaacct gaggcaaaat caattttgaa gttttatatc tttgagcaat
     3661 ttgccaaacg ttttcattcg ttacagttgg gccataagtc gttttctttt ttctgtcact
     3721 tcttgatgca gggccatgct ccactgtagt aatcacagtc ttttcaataa caccaggctc
     3781 attactgtaa cttgcggatt tcttcttgta ataaactggc ctgctttgag cggtggtaat
     3841 tcccaattga tatcctggcg ggtctaataa tactgtatag gccttgtata attgaccttt
     3901 tggccaggtt agatcgacga caatttccat atacggttct gtcattcttt ctgtagacga
     3961 tacttttaca acaggagtac cttttgggtt tctctcaacc tgaaaccgta acaaagacaa
     4021 gaccgcagtt cgctcaagtc caatttgttc aaattgctcc ggatcagcca gactcacctt
     4081 gatactggca agaggatctg ctcctacatc tattaattcg atttcggctg aaaaaggctg
     4141 atccaatgag gactgtacct tcatttcacc taagccaagc gcataaagag atattggcat
     4201 agtcaatgag aatatagccg catgcagaac agtttttttc aatcagagct ccatgcacct
     4261 gtgatacaag atgttgatga tttaattacc taagtaaaaa gtatcattat cctttagtct
     4321 attcaagaaa acacgaaatt attccctaaa aatcataaga tgatgtataa aattacatca
     4381 catatttcaa ctaatttaac aaggcaaatc aattgtggcc ttttaaaatc caaccaggaa
     4441 aattcataac ttcaatagac cttcaactat aattatatta aggattagta aaattaaatt
     4501 tggaatagcg atgaatagag aaaatgaagt gatcgaaata tttctaatgg atatcagcaa
     4561 aaaggaaaaa tgcaaattgt tacaagattt tcttctggat tgcaaaaatg aaatggaagc
     4621 tcaggatcag aacatgcatc ctgaagtgca tcacaattta tcgcaagctt atcaactggc
     4681 gcaaaattat ttaagaaaac tgcaagaata agtgtatttt ccttgttgca tatgaatcat
     4741 attgcattag aataacccgt aattcataat gtactccata aaaattgcga gaagaaaatg
     4801 cctgcatcac taatcgatgg aagagaaatc tctgcccttc gcagagctga actaaagcaa
     4861 agagtactat accatgtgga gcaagggcaa agggcgcctg gacttgctgt tgttttaatt
     4921 ggcaatgacc ctgcatcagt gatttatgtc agtaataaaa gaaaagcttg tgaagaagta
     4981 gggatcacct ctcattctta cgatttgcct gccgaaacaa ctcaggaaaa attaattgaa
     5041 ctgatcaatg agttaaatca atcggataaa attgatggta ttttaatcca attacctttg
     5101 cccaaacata ttaacgaaag aactataata gaatacataa aacccgagaa agacgtggat
     5161 ggatttcatc cttataattt aggccgactt gctcaacgaa acccgttttt aagaccatgc
     5221 actcccttgg ggattatgaa tttattacat cactatgagc tcaatgtaaa aagaaaacat
     5281 gctgtagtga ttggggcatc caatattgtt ggaaggccaa tgagtctgga gttactatta
     5341 gctggtgcta ccgtaacaat atgccacaag tttacccaac aattacagaa gtttgttgag
     5401 attgctgatt ttctaatagt agccactgga aagatggacg tcattgccac tgactggctc
     5461 agagagcatc aagtagtgat tgatgtagga atgcatcgtt tgcccgatgg cagtatcagg
     5521 ggtgatattg attttaagaa ggcagtcgaa aaagtagcct ggatcacacc tgttccaggg
     5581 ggagttggcc caatgactat tgtcactttg cttgaaaata ctatgatgtc agcagcaaga
     5641 ttaagagaat gaagttattt atccagttca tcccaatcaa attcaccact tagctcatca
     5701 aaatcgtctt tgaactcttc ttttagacgc ttacgttcaa gcctatcctc aagcatacgc
     5761 ctgattttct ttttttgatt taaatcatct atatgatcat caatttcttc gtcatagttg
     5821 taatctgaaa atgattgatc ctcaaactct tcatgtaaac ccatcttagt ctcccaattt
     5881 actcccctca aaaaaagtat agtgcataaa ttatgaaata aagtaactgg aagcaaaata
     5941 ggcctcttgc ataacctgat tattttgctt caggacatgg cgcatacgga gtttacatgc
     6001 agtacataag tgttgaagaa acgcttacga tgcgggagta aaataaaaga gaccgcttat
     6061 gcaagaagtc taatatgcgg ctacagcata aaccattatt ttgatataat ttggaaaata
     6121 tcttttgaag ccctctttca tgctcagtta tcaacatggc tatcatgccg gaaattttgc
     6181 tgatgtcatc aagcacatca ccctaactcg actattggct tatctaaccc acaaagataa
     6241 acccttattc tatcttgaaa cccattcagg tcgagggata tatgatttaa aagacaagca
     6301 gtctttaaaa acagaagaat ataaggaagg aattaatcca gtatggctcg atagagagaa
     6361 tctcccctct ctttttcttg aatatattag tgtaatcaag caaataaatc ttaattcaac
     6421 attgagttat tatcctggtt ccccctattt cgcaatcaat caactgcgct ctcaagaccg
     6481 attatactta tgtgaattac acccaacaga atataatttc ttattaaaac ttcctcattt
     6541 taataaaaaa gtttatgtta accacacgga tggagtttca aaattaaatg cccttttgcc
     6601 gccaccggaa aaacgtgggt taatttttat tgatccctca tatgaaagaa aagaagaata
     6661 caaagaaatt ccatacgcta taaaaaatgc ttactcaaaa ttttctaccg gcttatattg
     6721 tgtttggtat cctgttgtca ataaagcatg gactgaacaa ttcttgagga aaatgagaga
     6781 aattagttcc aaatcggtaa ggattgagct tcatttgaat ccactcatta atgaaggaat
     6841 gactggttgt ggtctttgga taattaatcc tccctatact ttcccttcag aaatcaagtt
     6901 agttttagag acattaacaa cttattttaa ccctggctca tcttcttata tgattgaatc
     6961 aggttcaaaa ttatgtcacg agttataaat tatgactatc ctgcctattt cggctttttc
     7021 agataattat atctggacct ttattgataa aatagctggg gtattagatt gtgttgatcc
     7081 aggagaagca gcaccaataa tacgctttgc ccaatccaac cagctgacac taagaacgat
     7141 attattaacc caccaccact atgatcatat aggaggtgtt gactcgctaa tcaagcaatg
     7201 gccatcttgt aaggtctatg gccccataga tgaacgaatc aataatgtaa cccacccaat
     7261 taaacaaggt caatctgttc aagtgggctc tcttcacttt catattttat ttaatccagg
     7321 gcatacttct acccatatca gttattatga accacaacaa ggatggttat tttgtggaga
     7381 tactctattt tctgctggtt gcggtagagt gtttgatgga actatagagg aattgcatga
     7441 atccctgctc ttatttaaaa aactcccgcg aaatactaaa gttttttgtg ctcatgaata
     7501 caccttccag aatttaaaat ttgcacatac cgttgagccc tgcaattcat cagtcatcaa
     7561 ttacatgcaa caaattctga aacaaccatc atcttgtact ctcccgtcta atatagactt
     7621 ggaattatca attaatcctt ttttacgcac tgataaagag caagtaaaac aatatgctct
     7681 ctcgcatggt gcgaattctt cagattctct ggacgttttt aaagtattga gaaatcaaaa
     7741 aaactcattc aaataatata acaaaaagat taaataaaaa cccatttgct cccaattaaa
     7801 ccaaaaaggg tttaatcctt cattaatttt tagtaaact
//
LOCUS       DACTJE010000156         7759 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.156, whole genome shotgun sequence.
ACCESSION   DACTJE010000156 DACTJE010000000
VERSION     DACTJE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.156"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(294..1130)
                     /locus_tag="I9760_002477"
     CDS             complement(294..1130)
                     /locus_tag="I9760_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044233.1"
                     /translation="MGKVNAVGMAEVGVERAKRKEALRVLTSSPFNLDNKEHTQALEK
                     YIKQFELSVNQMYLYQTMSTALTAWGGSWIVGRFLPIPEFANHLLAACLYLGVSGYIL
                     ESFSMTDFYEQLKEMKVLYNWCLKNNKENYSTDIDNTNNLLYPDIQRMIKLIAPLCST
                     DFMIAWPKEVKTAETQSGILSNTLSIGYSIVSAPFSLFSTASKPSQDQVQRIKDLKVQ
                     VETRGLDLGVFAGFEQAVKYFATNSDFRELLKAKLTQPVNSFKNLLQTTIPGMLAGSN
                     SK"
     gene            1276..2865
                     /locus_tag="I9760_002478"
     CDS             1276..2865
                     /locus_tag="I9760_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HAT5044234.1"
                     /translation="MPRTMFFSVDAANLQNLQITNQKAFAALESYLNDNDSPGAWNNN
                     QGYKYTHKDVTYCFSFNQSLVRRPRKSDQKKHTFEIFDPNKNPLGKGAYGIVYPILGT
                     IQFEFGNAIKRPPKNKLVKIQNHSERDQSHSVLREYQGLLQSGHIAVKPPIFVENKES
                     KFSYLIMEKAEGIVLEKFLNPIKRLDLSEEVPELNLINRIEITFAILKAIKEQVTDKH
                     LIHRDIKPGNIIIDFSKSPPIAKVIDFGFVLRASEQDYRRCGTRAYRAPESFNTQALY
                     TAKADVWSTGRILSYLWGDKYTNYYISRDKGLDYVIEKSRNELLFSDPELELYLTKED
                     KSKIRSCLSAMLIVDPEERGSIDKAIKQFSQIDFEKYKRLKRSTNFEIDLTDNDIKLR
                     KQLNSIHLHLITLQSKEKDLRNRACFDAANAMSQLVSKLTAYTNYLEKNPDPFLIKRY
                     KECCIAEIDLANLTLQNHRDALWLVAELSTAILLLGIGYLFAFGINYYNTGRLGLFSQ
                     TRSEKLVEEMKSSVLGIAVGN"
     gene            complement(2919..3728)
                     /locus_tag="I9760_002479"
     CDS             complement(2919..3728)
                     /locus_tag="I9760_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1484 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044235.1"
                     /translation="MPSMEMNCCIGKSLNYMRFSMSSFKHSCEKIDINLAHNVLTICP
                     KNREALQRITALFNNLYILYFIGGIPSITYDDMDQIQINIKYSQQITDAKLKNTLLSF
                     VNSELISQDEKETFLNELELTKTSFTTQFNTELENIRKKAEELSKKAEIDPYHYESAS
                     KAANRLHQELLIASQLYFYHKTPEAYENFKQTCQREIERARPVLEQHRGWKPLLVNVG
                     AAIIGLGVLYLLAASINYYKTEGRHFFFHFETDSLQKLENLNMAQSQVLRI"
     gene            complement(3927..4328)
                     /locus_tag="I9760_002480"
     CDS             complement(3927..4328)
                     /locus_tag="I9760_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044236.1"
                     /translation="MQESIHSLFLAQAIGLYLLIVGIIMLSRASYYRELLTHLKEGSA
                     TIVTAGSLGLIIGICLVLVHNIWVPESEVLVTLVAWLILIKSILWLGFPEFMVKCSQK
                     AYTGTGYYVISIVVLIIGILLITHGFYLFSH"
     gene            complement(4414..4935)
                     /locus_tag="I9760_002481"
     CDS             complement(4414..4935)
                     /locus_tag="I9760_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAT5044237.1"
                     /translation="MKNKPTKNYPNEKNTEYPIEILDKIDRKILNILQKNNQITNQAL
                     ADIVGISAPPCFRRVKRLREEKIIVNDVALVDPFKVGRPLIVFVNITLEKQREDLLAH
                     FERKMQEEPEVMQCYFVSGDTDYLLIIHVKDMNHYNEFARRVFANEPNIRKFRSSFCL
                     NRTKYSTQVVLDE"
     gene            5055..5585
                     /locus_tag="I9760_002482"
     CDS             5055..5585
                     /locus_tag="I9760_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5044238.1"
                     /translation="MITIRLAEMNEIQFLNQLISCSARELSQEDYTKEEIEGAIQYVF
                     GVDLELILDKTYFVIEKDGQTAGCGGWSRRKTLFGGSQYKGREQGIYLDPQQDFAKIR
                     AFFIHPKFARQGLGSILLKHCEHEALLQKFTRFEMMATLPGVKLYSAFGYHPISNEVI
                     TLPNNVPLRFVRMTKG"
     gene            5836..>7759
                     /locus_tag="I9760_002483"
     CDS             5836..>7759
                     /locus_tag="I9760_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044239.1"
                     /translation="MKEQQKAIFIENIKSTCAVDEQMAKELADSLLEIHQLNTGVNEQ
                     TLINEVFLTKFALYLKIGKEVALDWVRKNKDLDTPFPSNTVENQPAQDAKKPVENKVQ
                     VYGAMTVGSSPVNKTLNRLTNDLLKEVVERGKTQKAQKLRAYIFDQLARRLEASLSQE
                     QINDLYNRIRGTGDYTKSESFSEEQLKILKEKVVPELKRELSDLSNGNVNILGLDVSR
                     EDKYAFDTTNIFSVWFSNNPAVYMPQHVKTQVEKTAKLNQPGKTRIVFSSLCLNETAQ
                     IDFQQWAKENNIELVDIDSIDLKSVSETDAQLLNLAKDELGAMRKGKGGNPAAASDLV
                     RWVDVIIGESSTYIDIDLPMNDKKVTVEVHSGFPVLLNMGSALTKDGQQPAMENPVFN
                     TDMIAYSKDKEARRQIIEGVAKKIIARYENCAKYIEESKNEELVRLKNSPGYKLFVEK
                     TDGKFDLCTLRAAVSEAHQDALSFATFFGAEYFAKTFATQELIPVIKEAIQHQNQDLL
                     TSVIENHIEKQHLNDYPKTPDGIKKLLKSFQGIVYKPLVMEFSGPSAVSSSWVEAISG
                     RSIPRNFEYLAEPMSQPLRVLQHYACVSGKANFSSDNIPKWCELQSDVEKRQQQREDG
                     LSWLPDAQEKLKREGQQ"
BASE COUNT         2551 a         1298 c         1448 g         2462 t
ORIGIN      
        1 cattctttta atttgctgat atcagatatc tgctctagaa acattttttt gtattgattg
       61 taaagcaatt ccttgtttgc aaaaatagtt ttcatcttga gaaacctttg tggagtaaat
      121 caatgacatt atttgctgtt aatcatcctc atattggcct taatgatgat tatgcctcct
      181 ttctttaatg ctaatgccaa cctttaataa ggcttaaggt cggcaagaaa aacatcagct
      241 ctttacctaa tacgatcgtt gggtaatatt taagcatgag ggatctatta tcattatttt
      301 gaattagatc ctgccaacat tcctggtatg gttgtttgca acaaattttt aaatgagttg
      361 acaggttgag ttagcttggc tttcaataat tctctaaaat cagagttcgt agcaaaatac
      421 ttgactgcct gctcaaaacc agcaaaaacc cctaagtcaa gacctcttgt ttctacctgc
      481 acttttaggt ctttaattcg ttgcacttga tcctgactcg gtttactggc tgttgagaac
      541 agtgaaaatg gcgctgatac tatagaatag cctatcgaca atgtgttgga tagtattcca
      601 ctttgagttt ctgctgtttt tacttcttta ggccaggcaa tcataaaatc agtagagcac
      661 aaaggggcga ttaatttaat catgcgctgg atatcaggat acaaaaggtt attggtatta
      721 tcgatatcgg tactatagtt ttctttgtta ttttttagac accaattata gagtactttc
      781 atctctttca attgttcata aaaatcggtc atgctaaatg attcaagaat gtaaccagaa
      841 acgcctaaat agagacaggc tgcaagaagg tgattcgcaa attcaggaat aggcaaaaac
      901 cttccaacta tccacgagcc cccccaagct gttaatgcag tactcattgt ttgatagagg
      961 tacatttgat tgacacttaa ctcaaattgt ttgatgtact tttccaatgc ctgagtgtgt
     1021 tccttattat ccaggttgaa gggagatgac gtcagaactc ttagggcttc tttcctcttg
     1081 gctctttcaa ctccgacttc agccattcca actgcgttta ccttacccac aattctctcc
     1141 ttgaaatcaa aatttcattt tatactataa atttaaaaaa taatgaatta gattttagct
     1201 aagagttcaa ttaatttaac ttaataaaaa agacgaggta taataaaacc gatcaatcaa
     1261 attgaagtca atatcatgcc tcgtaccatg tttttttccg ttgatgcagc taatctccaa
     1321 aatttacaaa ttacaaatca aaaagctttt gcagcgttgg agagttatct gaatgataat
     1381 gattcaccag gtgcctggaa taataatcaa ggatacaaat atactcataa ggatgttact
     1441 tattgcttct catttaatca atccttagtg agacgcccaa gaaaatcgga tcaaaaaaaa
     1501 cacacatttg aaatttttga ccctaataag aaccctttag gcaagggagc gtacggtatt
     1561 gtgtatccca ttttaggtac aattcagttt gaatttggaa atgcaattaa aagaccacca
     1621 aagaataaat tagttaaaat acaaaaccat agtgaacgcg atcaaagtca ttctgtacta
     1681 cgtgaatatc aaggacttct tcaatcaggg catatagcgg ttaaaccgcc aatatttgta
     1741 gaaaacaaag agagtaaatt cagttacttg attatggaaa aagcggaggg tatcgtttta
     1801 gagaagtttc taaatcccat taagcggctt gatttgtctg aagaggtacc tgagcttaat
     1861 cttattaatc ggattgagat tacttttgcc attttaaaag caattaaaga gcaggtgacg
     1921 gataagcatt tgattcatcg agatataaaa ccaggaaata ttataatcga ttttagcaag
     1981 tcgcctccga tagctaaagt aatcgatttt ggttttgtgc ttagggcgtc agaacaggat
     2041 tatcgtcgtt gtggaacccg agcatacaga gcgcccgaat ctttcaatac ccaggcattg
     2101 tacacagcaa aagctgatgt ttggtcaaca ggcagaatat taagttattt gtggggagat
     2161 aaatatacta attactatat ctctcgtgat aagggtctgg actacgtgat tgagaagtca
     2221 agaaacgagc tactcttctc tgacccggaa ttggaactct atttaaccaa ggaagataaa
     2281 agtaaaatca gatcgtgcct aagcgcaatg cttatagttg accctgagga aagaggatct
     2341 atagataagg ctatcaagca gttttcccaa attgattttg aaaaatataa aagattgaaa
     2401 cgctctacaa attttgaaat tgatttaaca gataatgata ttaaattaag aaaacagctc
     2461 aattcaattc acctgcattt gattacccta cagagcaaag aaaaagattt gcgtaataga
     2521 gcatgttttg atgcagcaaa tgcaatgagc caattggtta gtaagctcac cgcctacacc
     2581 aattatcttg aaaaaaatcc agatccattt ttaataaaac gttataaaga gtgctgcata
     2641 gcagaaatag atttagccaa tttgactttg caaaatcacc gtgatgcctt atggttagtg
     2701 gctgagttat caactgctat ccttttgctt ggtatagggt atttatttgc ttttgggata
     2761 aattattaca acacaggtcg tctgggatta ttttctcaaa ccaggtctga gaaattggtg
     2821 gaggaaatga aaagctctgt gttgggtatt gcggttggaa attaaaaatc tgtatttccc
     2881 ggattataac agagccgtct aatccgggtt gttcatggtt agattcttaa cacttgagat
     2941 tgagccatat tcaggttttc taatttttgc aaagagtccg tttcaaaatg aaagaaaaaa
     3001 tgacggccct ctgttttata atagttaatg gaagctgcaa gcaaatagag aacaccaagg
     3061 cctatgatgg ccgctcctac attaactaat aaaggcttcc atcctctatg ctgttccagt
     3121 acaggtcgag ccctctcaat ttctctttgg catgtttgtt tgaaattttc gtatgcctcg
     3181 ggtgttttat gataaaaata cagttgggaa gctattaata attcctgatg cagtctgtta
     3241 gctgcttttg atgcagactc ataatgataa gggtcaattt ctgctttttt gcttaactcc
     3301 tcagcttttt ttctgatatt ttccaattca gtattaaatt gggtagtgaa tgaagttttg
     3361 gtgagctcca gctcatttaa aaatgtttct ttttcgtctt gagagattaa ttcagaatta
     3421 acaaaagaaa gtaatgtatt tttgagtttg gcatcggtta tttgttgaga atacttaata
     3481 ttaatttgaa tctgatccat gtcatcatag gtgatgctag ggatacctcc tataaaataa
     3541 agaatgtaca gattattgaa aagagcagta attctttgca gcgcctctct atttttagga
     3601 caaatggtta gaacattatg agctagattg atatcaattt tttcacaaga atgtttaaat
     3661 gatgacatac taaacctcat ataattcaga gatttcccta tgcaacaatt catttccatt
     3721 gaaggcaggt caagtatatg tagacaagct taagatttta ttaagaagcg tcttaaattt
     3781 aacaattttt caaaaaaact ttacgatttt ttgaaatgca aaaaatatcg ttaactcttg
     3841 cagatttcct ttataaaaac ctacacactt tatccacttg atggttacct tgctgaaagc
     3901 agagttgctt atcaaagcag gtaaaatcaa tgggaaaaca ggtaaaatcc gtgggttatt
     3961 agcagaatcc caataattaa taccactatg gaaatcacat aataacctgt ccctgtatag
     4021 gctttttgac tgcatttcac cataaactcg ggaaatccaa gccataatat tgatttaatc
     4081 aaaataagcc aggctaccag tgtaactaat acctctgatt caggaaccca aatgttatgg
     4141 accagtacaa ggcaaatacc gataattaac cccaagcttc cagccgttac aatagtggca
     4201 ctgccttctt tgagatgagt taacaattca cgataatatg aagctcgaga aagcattata
     4261 attcccacaa taagaagata gaggccgatt gcttgtgcca aaaataaaga gtgaattgat
     4321 tcttgcatgg ttggctcctt gtgattccgc tcctaaatta attatagtac atgcgggatt
     4381 gggtttgtta accaggcagg tacttgctag ttactactca tctaaaacaa cttgtgtact
     4441 atatttggtt ctattaagac aaaagctgct tctgaatttt ctaatattgg gctcattagc
     4501 gaatactcgc ctggcaaatt cattgtaatg attcatatct ttgacatgaa taataagcag
     4561 gtaatctgtg tctcccgaga caaaatagca ttgcataacc tcaggctctt cctgcatttt
     4621 tctttcaaaa tgagctagca gatcttctct ttgtttttca agagtaatat tgacgaacac
     4681 aattaatggt ctgcctactt taaatggatc aaccaaagca acatcgttaa cgataatttt
     4741 ttcctctctc aggcgtttga ctcttcgaaa gcagggtggg gcagagattc caactatatc
     4801 tgccaatgcc tggttagtaa tttgattatt tttctgaaga atatttagaa tttttctatc
     4861 aattttatca agtatttcaa ttggatattc tgtatttttt tcatttggat aattttttgt
     4921 tggcttgttt ttcatatatt accttttgtt gcaaaaatac attataaaaa gtaattttta
     4981 taaataaaat aagaaattta tttatatttg ttttatgtta acttacctcc acacagactt
     5041 gcaggaggtt accaatgatt acaatcagat tagctgaaat gaacgagatt caatttttaa
     5101 atcaattaat ttcttgttca gcaagagagc ttagccagga agattataca aaagaagaaa
     5161 ttgaaggagc tattcaatat gtatttggtg tagatttaga gttaatttta gacaaaactt
     5221 attttgtgat agaaaaggat gggcaaactg ctggttgcgg aggatggagc cgaagaaaaa
     5281 cattattcgg tggcagtcaa tataaaggca gggaacaagg aatatatctg gatcctcaac
     5341 aagattttgc aaaaataaga gcttttttta ttcatcccaa attcgcaaga caaggattgg
     5401 gaagtattct gttaaaacac tgtgagcatg aggctctttt acaaaaattt actcggtttg
     5461 agatgatggc cactttaccg ggagttaagc tctatagcgc ttttggttat caccctatct
     5521 caaatgaagt gattactctc cccaataatg ttccgttaag atttgtccgg atgacgaaag
     5581 gttagatggt aattcatttt atattgtctg gagtcattat tgactcatgg ttattgaaac
     5641 ttaatttatc atcataatgt taggaacggt tatcttaatg aatacaatcc gattagatta
     5701 ataacgtaat ttaagtttta cttaagttat ttatgatata ttcatgacca ctttgtaaat
     5761 atttaattta tttttggtgt tttctccagt tgagacaaca ggaaaatata gattataacc
     5821 aggtgggata aatatatgaa agagcaacaa aaggcaattt ttattgagaa tatcaaatcg
     5881 acatgcgctg ttgacgagca aatggctaaa gaattggctg attcattgct tgaaattcac
     5941 cagttaaaca ctggagttaa tgaacaaaca ctgattaatg aggtgttttt aactaaattt
     6001 gctctctatc ttaaaattgg gaaagaggtt gctttagatt gggttcgaaa aaataaggac
     6061 ttggatactc cctttccttc taatacagtc gaaaatcagc ctgctcaaga tgcgaaaaag
     6121 cctgttgaga acaaggttca agtatatgga gctatgacag ttggcagttc tcctgttaat
     6181 aagactttaa atcgattaac caatgatctt ttgaaagagg tcgttgaacg tggaaagact
     6241 cagaaagctc aaaaattacg agcttacata ttcgatcaat tggcaaggcg attagaagct
     6301 tcgcttagcc aagagcagat taacgattta tataacagaa taagaggcac tggtgactat
     6361 actaaaagcg aatcattttc cgaagaacaa cttaaaattt taaaagaaaa agttgttcca
     6421 gaattaaaga gagagctaag tgacctcagc aacgggaatg ttaatattct ggggttggac
     6481 gttagtcgtg aagataaata cgcatttgat acaaccaata tattttctgt ctggtttagt
     6541 aataacccag cagtttacat gccacaacat gttaaaactc aagtagaaaa aactgcaaaa
     6601 cttaaccagc caggcaaaac tcgaattgtt tttagctcat tatgcttgaa tgagactgca
     6661 cagattgatt ttcagcaatg ggcaaaggaa aacaacattg aattggttga tatagattca
     6721 atagatttaa aatcagtcag tgaaactgat gctcaattgc taaatctagc caaagatgaa
     6781 cttggagcaa tgagaaaggg caagggagga aatcctgccg cagcatctga tctggtgcgt
     6841 tgggttgatg taatcattgg agaaagcagt acttatattg atattgattt accaatgaat
     6901 gataaaaaag tcacagttga ggttcattcc ggtttcccag ttctacttaa tatggggtca
     6961 gcgctcacaa aggatggtca acagcctgct atggaaaacc cggttttcaa tactgatatg
     7021 attgcttata gcaaagataa agaggcacgt cgtcaaataa tcgagggggt tgccaaaaag
     7081 atcattgcgc gttatgaaaa ctgcgcgaaa tatattgagg aaagcaaaaa cgaagagtta
     7141 gtacgtttaa aaaattcacc aggttataaa ttatttgttg aaaaaacaga tggcaaattt
     7201 gatttatgta ctttaagggc ggcggtttca gaagctcacc aggatgcttt gtcgtttgca
     7261 actttttttg gcgctgagta ttttgctaaa acttttgcta cacaagaact tatacctgta
     7321 attaaagagg caattcagca tcaaaatcaa gatttgctta catctgttat tgaaaatcat
     7381 atagaaaaac aacatctcaa tgactatcct aagactcccg atggcattaa aaaactattg
     7441 aagtcgtttc aagggattgt ctataaacca ttggttatgg aattctcagg cccaagtgcc
     7501 gtctcatcat cttgggtgga agctatatct ggaagatcca tcccgagaaa ttttgaatat
     7561 ttagctgaac ctatgtctca accattacga gttttacagc attatgcctg tgtatctgga
     7621 aaagctaatt tttcttctga taacattcct aaatggtgtg aacttcagtc tgacgtagaa
     7681 aaacgacaac aacaaagaga agatggactt tcctggcttc ctgatgctca agagaagctt
     7741 aagagggaag ggcagcaag
//
LOCUS       DACTJE010000157         7695 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.157, whole genome shotgun sequence.
ACCESSION   DACTJE010000157 DACTJE010000000
VERSION     DACTJE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.157"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..418)
                     /locus_tag="I9760_002484"
     CDS             complement(<1..418)
                     /locus_tag="I9760_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAT5044240.1"
                     /translation="MRIGIAILVVLVCCTLQGCKKEKSPYQTTSYVESQLFIVSSPDG
                     GYIKEWYADEGQLLHKEDKIVTLDGVNSIKAPADSVMTERYYLKDEYVPPNFPIASLS
                     LPSQMKILFYVPESHLEKIKLGKKIRILLNEKKYSGK"
     gene            complement(486..1697)
                     /locus_tag="I9760_002485"
     CDS             complement(486..1697)
                     /locus_tag="I9760_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAT5044241.1"
                     /translation="MYANYYDMYQKTGIFSDEFFGQFLRTLGKCYVQCADVGECYATL
                     SKITDKDGESWYKAWYEMANTLKTKANISWEANHFITAAYAYLRAAEYYRASEFFLRA
                     NPDDPRVIACFDQLQFCFERAMHRLHPGAQKIWIPYDDQTLSGYLFFSDQSPKATLIV
                     PGGYDSTFEENYALVPAALLHGYHILIFDGPGQGHVLRRQKLYMRADFEHVVSQVVDW
                     LDEQSELGTSSYVLIGRSFGGYLAPRAACSEKRLAALICDPGQMDIAGSLNKMLPPNL
                     MMLLKEGKADELNACFYQLFSENPMKEFYFKSRMYTHGLATPFDYLQALSAYTFVDRV
                     QEITCPTLVCDNPSDRISNRGNTLYEALRCEKNYVMFDAKLGAGMHCEADANGQFQQV
                     IFDWLDERFTQ"
     gene            complement(1962..4703)
                     /gene="glnE"
                     /locus_tag="I9760_002486"
     CDS             complement(1962..4703)
                     /gene="glnE"
                     /locus_tag="I9760_002486"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAT5044242.1"
                     /translation="MSPQSDIETLISTKSWLIEKYVAPLHHPLRESAEKLILVSDYAC
                     RQIRLLVSLLESDSCCSLWSRESYFYAIQQISLELSQAQYMQELRRFRHTHFLRLLLL
                     EIADLASTQEVMQSWSDLADSIILHTLKYIGFTLLSRYGVPRNSEGKEIQLYALAMGK
                     LGGRELNYSSDIDLIFAFNEVGSTDGEEQITNQHYFSKMVQTFVHLLQSVTPDGFVFR
                     VDLRLRPNGDSGPLVSSFAAMETYYQEQGRDWERYAMVKARVIAESLHEPFPWFERLI
                     IPFVYRRYVDFGVIESLRGMKAMIEREVQLNPLLNDIKRGRGGIREIEFIIQNIQLIR
                     GGRLPQLQVQNAMLALAVIKKEKLLPRCDALKQAYLFLRKLENILQSLNDQQTHSLPE
                     DQLKKVQIVYAMGFTDWEQFFNKLQQYQRIVSNFFHSILGKVDDYEDEKRLLNNQLFS
                     LWQGHVESNMAVNLLISLGYENAQHCYQMLHAFRHGPRCRRLSQGARIRLDRFMVLLL
                     TELTYCPKTDEVLLQVIHLLENIVGRSAYLALLTENPHALRELLFWFVNSPFISSLFV
                     SQPFLLEILLEQEKDWHPDSLSQMEQTIVEKLEHTNDVEVHEEILRQFKLTNWMVAAR
                     AELYGLCDAVRIGKFLSDVAQVIVSQVLIIASKQLCGRHPEMAQIQSRFAIIAYGKLG
                     SREMSYASDLDLVFLHSAKPSEEALVTRLTQKILHMLTTRTQMGVLYHVDTRLRPSGA
                     AGLLVSHVDAFVEYQKTQAWTWEHQALLKARILSGNSKIKNQFSQLKKSVLLLERDSK
                     LLQRDVLTMRSKIDQFQESDEVKLARGGLLDLEFLVQFLILNLRDPKFSSYTNTLSQL
                     KHLFLSNVLTKSQFTTLKKAYQYFHYLMHQKIVQSDVINDDEMQNAVSSVCKDLYRY"
     gene            4841..5593
                     /locus_tag="I9760_002487"
     CDS             4841..5593
                     /locus_tag="I9760_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAT5044243.1"
                     /translation="MKFIQGLLLSFFFLVSPLHAQNEPIPLIVGVGGFYPPFIMQGTN
                     NELFGYDIATMTELCKIMKRQCNFRVMRFRQLIPALINKQIDVAISAMSITSERSNLV
                     NFSMPYLLSYSRFLTNEVHNTHQPFNLMLLKGKRIGVEQNTVFSQEVRRIGIIDPVIV
                     EFDRTEEMLEALSANKIDFVLMDNPAALYWSANSSGRLLVIGSPMLVGYGLGIAVNRE
                     NSVLLKSLNDALVEYQRSDKYKENYNKYMMNF"
     gene            complement(5848..6114)
                     /locus_tag="I9760_002488"
     CDS             complement(5848..6114)
                     /locus_tag="I9760_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044244.1"
                     /translation="MSQNWPTRDKDLQTARVIMEEYANERDSDSLGLFEIVVDQNEKR
                     MNFRLSGWVVILAKHFTSLYGVSQGDYVTRQVISRCITQGQTLH"
     gene            6412..7239
                     /locus_tag="I9760_002489"
     CDS             6412..7239
                     /locus_tag="I9760_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HAT5044245.1"
                     /translation="MHKIINGAMTGIGLRAPHYAQILEEKPDIDWLEVHTENFLVKGG
                     PLLELLDSISQHYPLSFHGVGLSLGSAEGVCIAHLNRIKTLIDRFQPALLSDHLSWST
                     IGNTYLPDLLPIPYNEESLTIISNNIDRVQNYLKRTLLIENPSSYLEYQTSSYSEPDF
                     LAEIIKRTEARLLLDINNIYVSCSNNKQDPYQYIQRIPHEAVQEIHLAGHSIVYQDNT
                     NFLRIDTHNNYVCHEVWDLYRYAIHYTGLVPTLLEWDTEIPSLDTLLNEARKSACYA"
     gene            7232..>7695
                     /locus_tag="I9760_002490"
     CDS             7232..>7695
                     /locus_tag="I9760_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HAT5044246.1"
                     /translation="MPRMKQIQEFFLNTVLNQQDTAIFKYFCPNEITNEFRFDIYRNT
                     ILQNLRHSLEMTFPAIWKLVGKGCADGLALHFIQDKNNLPTTNCLDDWGEKFPLFLKN
                     NPTVSHLLYLKNIAEIEWLKHLSYCSRDCLVFDPQKLQKHLNDRVEKLILLF"
BASE COUNT         2491 a         1552 c         1304 g         2348 t
ORIGIN      
        1 ttttgcctga atattttttt tcattaagca aaattcgaat ttttttacct aatttaattt
       61 tttccaaatg gctttcggga acataaaaca gaattttcat ttgagaaggt agagagagac
      121 tcgcaatagg gaaattagga ggtacgtact catcctttaa ataataacgt tcagtcataa
      181 cagagtctgc gggcgctttg attgaattta ccccatcaag ggttacaatt ttatcctctt
      241 tatgcagtaa ttgaccttca tcagcatacc attcttttat gtatccacca tccgggcttg
      301 acacaataaa taactgagat tcaacatagc ttgtcgtttg atacggtgat ttttcttttt
      361 tacaaccttg caatgtgcaa caaactaata caaccaggat tgcaatccct attctcatac
      421 ctcttatcct tttgtcatca gtattagtta tcctatcagc aaagcaataa cgattgggat
      481 acccactatt gggtaaatct ctcatccagc caatcaaaaa taacttgctg aaactgacca
      541 ttagcatctg cttcacaatg catacctgcc cccagtttgg catcaaacat cacatagttt
      601 ttttcacaac gcaaagcttc atatagcgta ttcccacgat tggatatcct gtcgcttgga
      661 ttatcgcaga ccagtgtggg acaagtgatc tcttgcaccc tatcaacaaa ggtgtatgca
      721 gataatgcct gcaaatagtc aaacggagta gccaagccgt gtgtatacat tcttgatttg
      781 aaataaaatt ctttcattgg attttctgag aacaattgat aaaaacaggc attaagttca
      841 tcagcttttc cttcttttaa taacatcata aggtttggag gtagcatttt attgagtgaa
      901 cctgcaatat ccatctgtcc tggatcacaa attaatgcag ctaatcgttt ttcactgcag
      961 gccgcccttg gtgcaagata accgccaaaa cttctgccaa tgagcacata actggaagtc
     1021 cccagttcac tttgttcatc taaccaatcc acaacctgag agacaacatg ttcgaaatca
     1081 gctctcatat acagcttttg tcgacgcaaa acatgccctt gtcccgggcc atcaaaaatc
     1141 aaaatatgat acccatgtaa aagcgcagca ggaacgagag cataattttc ttcaaaagta
     1201 ctgtcatatc ctccaggtac tatcaaagtt gctttgggag attgatcgct gaaaaataaa
     1261 taaccgctta atgtttgatc atcataaggg atccatattt tttgagctcc aggatgcaat
     1321 cgatgcattg ctcgttcaaa acaaaattgc aattgatcaa aacaagcaat cacacgcgga
     1381 tcatcaggat tggcacgtaa aaaaaactcg cttgcccggt aatattctgc agcacgcaga
     1441 taagcgtatg ctgctgttat aaaatgattg gcttcccaac tgatattggc tttggtttta
     1501 agtgtatttg ccatctcata ccatgcctta taccaacttt ccccgtcttt atcagtgatt
     1561 ttggacaagg ttgcataaca ttcaccaaca tcagcacact gcacataaca tttaccaaga
     1621 gttcttaaaa actgtccaaa aaattcatca ctgaaaatcc ctgttttttg atacatatca
     1681 taataattag catacataac agtaaaaaat tccctaaaaa ttacagagta actgaaagta
     1741 aaatggattg ccatgtgtca gagtatatta cgcgcaggcc tatgaattat cctaaatgat
     1801 attaggacag acttacgaaa accccaaggt caaggcaaaa aatgttttta atgagggagt
     1861 ttagataaac taaatgactg aattaaaaac attttttaac gacgagattg gggggcaaat
     1921 ctgtaggatt cattttcagg ggagtgctgt ctaaacacaa tctaataccg ataaagatct
     1981 ttgcaaactg aagacactgc gttttgcatt tcgtcatcat taataacatc tgactgtaca
     2041 attttctgat gcatcaaata atgaaaatac tgataagctt tctttaaagt tgtgaattga
     2101 gatttcgtta atacattcga taaaaacaaa tgcttaagtt ggcttagtgt attggtataa
     2161 cttgaaaatt tgggatccct aagattcaat ataagaaatt gaaccaaaaa ttccagatcc
     2221 aataatcccc cacgagctag ctttacttca tctgactctt gaaattgatc gattttagat
     2281 cgcatggtta atacgtccct ttgcaataat ttactatctc tctctagcaa caggactgac
     2341 ttttttagtt gggaaaactg atttttgatt tttgaatttc ccgataatat cctcgccttt
     2401 aacaaagctt gatgttccca tgtccaagct tgtgtttttt gatactcaac aaaagcatcc
     2461 acatgactga ctaacaagcc tgcagcaccg gaaggtctta aacgagtatc aacgtgatac
     2521 aaaaccccca tttgagtacg agtagtcagc atatgcaata ttttttgtgt taatcgggtc
     2581 actaaagctt cttcagatgg tttggcagaa tgtaaaaaga ccaaatcgag atcagacgca
     2641 tagctcatct ctctactgcc cagttttcca taagcaataa tggcaaatcg cgattgaatt
     2701 tgagccattt cagggtgacg gccacatagc tgtttgcttg caataattaa aacttgactg
     2761 acaatgactt gcgccacatc agatagaaat ttccctatgc gcacagcgtc acataatcca
     2821 tacaattccg ctctggcagc caccatccaa ttcgttaatt taaactgacg caggatttcc
     2881 tcatgaactt ccacatcatt agtatgctct aatttttcaa ctattgtctg ttccatttgg
     2941 gataatgaat caggatgcca atccttttcc tgctccaaca agatttccaa caaaaaaggt
     3001 tggctaacaa acagggaaga aataaaagga ctattcacaa accaaaataa taattctctt
     3061 agcgcatgcg ggttttcagt gagtaaagcc aggtaagcgc ttcgccccac aatattttca
     3121 agcaaatgaa ttacttgtaa taaaacctca tccgttttag ggcagtaagt caattctgta
     3181 agcaataaaa ccataaaacg atcaaggcga atgcgagcgc cctgtgataa tcttctgcac
     3241 cgtggtccat gtcgaaaagc atggagcatt tgataacaat gttgtgcatt ttcataccct
     3301 aaactaatca ataaattcac tgccatatta ctttcaacat gaccttgcca aaggctaaat
     3361 aattgattgt ttaacaatct cttttcgtct tcataatcat caaccttgcc aagaatggaa
     3421 tgaaagaaat tactaacaat tcgttgatat tgttgtaatt tattgaaaaa ctgttcccaa
     3481 tcagtgaatc ccatggcata aactatctgc acttttttta actggtcttc aggtagagaa
     3541 tgggtttgtt gatcgttcaa actctgtaga atattttcca atttcctcaa aaaaagatac
     3601 gcttgtttta aagcatcaca cctgggtagt aatttttctt tttttatcac agccagcgct
     3661 aacatggcat tttgaacttg caattgaggt aatctgcctc cgcgaattaa ttgaatattc
     3721 tgaataataa attctatttc cctaatacca ccccggcctc gtttaatatc attcaataag
     3781 gggttaagtt gcacctcacg ctcgatcatt gccttcatcc ctcgcaggga ttctatcact
     3841 ccaaagtcca catacctcct gtaaacaaac gggatgatta aacgctcaaa ccatggaaat
     3901 ggttcatgca aagactcagc aataacccgc gctttaacca tggcataacg ttcccaatca
     3961 cgcccctgtt cctggtaata ggtttccata gctgcaaaac tggaaaccaa aggaccacta
     4021 tctccattag gtcgtaatct taaatccact ctaaatacaa aaccatcagg cgttacactc
     4081 tgtaataaat gaacaaatgt ttgcaccatc ttgctaaaat aatgctgatt ggtaatctgc
     4141 tcctcaccat cagtgctgcc aacttcattg aaggcgaaaa tcaaatcaat atcggacgaa
     4201 taattaagtt ctcttccccc aagtttcccc atggcaagag catacaattg aatctcttta
     4261 ccctcactgt ttctgggaac cccgtaacga ctcaacaagg taaatccaat atattttaaa
     4321 gtatgcaaga tgatagagtc tgccagatca gaccaggatt gcataacctc ttgagtagaa
     4381 gccaaatcgg ctatttcaag taataacaac cgcaaaaaat gggtatgacg aaaccgacgc
     4441 aattcctgca tatactgggc ttgagataat tccaggctga tttgctgaat ggcataaaaa
     4501 tagctttcgc gagaccataa ggaacagcag gaatcacttt ctaaaagaga gaccaataat
     4561 ctaatttgtc tgcaggcata gtcacttacc aatatcaact tttcggcact ttctctcaat
     4621 ggatggtgca aaggagcaac gtacttttca atcaaccagg attttgtaga aatcaatgtt
     4681 tcaatatcgc tttgtggaga cataaaatat actattctta atataaaaac ctaaacatat
     4741 cataggatta atcaattaat tttacattta atactttaaa actcctattg gacgttatac
     4801 tcttataaaa gactctaata atattttagt gaagatacca atgaaattta ttcagggatt
     4861 attgctttct ttcttttttc tagtttcacc tctccacgct caaaacgagc ctataccttt
     4921 aatagtaggt gtaggaggct tttaccctcc ctttatcatg caaggaacca ataatgagct
     4981 ttttggctat gatatcgcca ccatgacaga actgtgtaag ataatgaaac gacaatgtaa
     5041 ttttcgagtc atgagattca gacaattaat tccggctctg attaacaaac aaattgatgt
     5101 ggcgatcagc gccatgtcta ttacttcaga acgttcaaac ctggttaatt tcagcatgcc
     5161 ctatttattg agttactcca ggtttcttac taatgaggta cacaatactc atcaaccgtt
     5221 taatttaatg ctcttaaaag gtaaacgaat tggagtggaa caaaatactg tctttagtca
     5281 ggaagtcaga aggataggga taatagaccc tgtcatagtg gagtttgaca gaaccgaaga
     5341 aatgcttgag gcattgagcg caaataaaat tgactttgtt ctgatggata atcctgccgc
     5401 tttatactgg tccgctaatt cgtcggggag acttctggta ataggcagtc ccatgctggt
     5461 tggttatggt ttgggaattg cagtcaatcg ggaaaactcc gtattactca aatccctaaa
     5521 tgatgccttg gtagaatacc agcgttccga caaatacaag gaaaattaca ataaatacat
     5581 gatgaatttt taagagtgat gtggttgggg caattgctac aaaaattgct cttaatcatt
     5641 catcaatagc tgaataatcg ttttaaggct taataagtca acatcctgat atttaactat
     5701 tctctaaggg caggatctga gtccaggttg gatgaaagta atcatgaaaa atcacagcat
     5761 agatagctat gtgagcattt cgaaggatta ttttctttca atatgaaccc aatacggcca
     5821 ttccaattga gggctcatct ctagctttta atgcagagtt tgaccttgag taatgcagcg
     5881 actaatcacc tgcctggtca cgtaatcacc ctgactaact ccatataaag aagtaaaatg
     5941 ttttgccaaa ataactaccc aaccagataa acggaaattc atgcgttttt cattttgatc
     6001 aacgacaatc tcgaaaagac ctaatgaatc actatcacgt tcattagcat actcttccat
     6061 tatgactcgc gccgtttgca aatctttatc tctggtaggc caattttgac tcataagaca
     6121 ctccctaaaa acatgagcaa gactgttgtt acttctacaa catggaggca attttgaaaa
     6181 ttgcaagttg tgaaataaat aaatattatc tcataaaatg attaaatact caatattgcg
     6241 gttaattttt acactgaatt aaaaacattt atggtttaaa atacccattg aaaatggctt
     6301 tctccaagac cattgcgttc gaaacttctc aagagtacat taatatatca ttaaatcttt
     6361 gacatagttt tttgattgat caacaaaaat tcaaatcgga taaaaaatat catgcataaa
     6421 atcataaatg gtgctatgac aggtattggc ctaagagcgc cacattatgc ccaaatatta
     6481 gaggaaaagc ctgatattga ttggcttgaa gtacatacag aaaatttctt ggtcaagggt
     6541 gggcctttac tagagcttct agattctata agccaacatt atccactgag ctttcatgga
     6601 gtcgggttat ctttagggtc tgctgaggga gtctgtatag cgcatttaaa tcgcattaaa
     6661 actctcattg atcgctttca accagctttg ctttctgatc atttgtcctg gagcaccatc
     6721 ggaaacacct atttacccga tttattacct attccctata atgaggaaag tttaaccatt
     6781 attagcaata atattgatag agtgcaaaat tatttaaaga gaaccttatt aatagaaaac
     6841 ccctcttctt atttggaata tcagacttca tcgtattcag agcccgattt tttagcagaa
     6901 ataatcaagc gtactgaagc caggctcttg ttggatataa ataatatcta tgtttcctgt
     6961 tctaataaca aacaagatcc ttatcaatat atccaaagaa tcccgcatga agcagtacaa
     7021 gaaattcatt tggcgggcca ttccattgtc tatcaggaca atacgaattt cttgcggata
     7081 gacacacata ataattatgt gtgtcacgag gtatgggatt tgtatcgata tgcaatacat
     7141 tatacaggcc ttgttcccac gttgttagaa tgggatacag aaattccctc gttagatact
     7201 ttattaaatg aagccaggaa gtccgcctgt tatgcctaga atgaaacaaa tacaggaatt
     7261 ttttctgaac acagtattaa atcaacaaga tactgccatt tttaagtatt tttgccctaa
     7321 tgagattact aatgaattcc gtttcgacat ttatcgtaat accattttgc agaatctgcg
     7381 gcattccctg gaaatgacct ttccagccat ttggaaattg gttggcaaag gatgtgcgga
     7441 tggtctggca ttacacttta tccaggataa aaataatttg ccaacaacca attgtttgga
     7501 tgattggggt gaaaaatttc ctctgttttt aaaaaataat ccaaccgtta gccatcttct
     7561 ttatttgaaa aatatcgcag aaatagaatg gttaaagcat ttaagctatt gctcgcggga
     7621 ttgtcttgtt tttgatcccc agaaactgca aaaacaccta aatgatcgag tagaaaaatt
     7681 aattttgcta tttaa
//
LOCUS       DACTJE010000158         7670 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.158, whole genome shotgun sequence.
ACCESSION   DACTJE010000158 DACTJE010000000
VERSION     DACTJE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.158"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            362..556
                     /gene="csrA"
                     /locus_tag="I9760_002491"
     CDS             362..556
                     /gene="csrA"
                     /locus_tag="I9760_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAT5044247.1"
                     /translation="MLILTRRIGETLIIGDDVNITVLGVKGNQVRLGINAPKDVSVHR
                     EEIYLRIQQEKESDDSEQAV"
     gene            complement(652..1644)
                     /locus_tag="I9760_002492"
     CDS             complement(652..1644)
                     /locus_tag="I9760_002492"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAT5044248.1"
                     /translation="MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQL
                     NDLGIPITRIPHQGYQLEKPLILLDSNEITKQLHSEGFSQPFNLHLFTSIDSTNRYLK
                     DLPSSPAVEICCTEMQTQGRGRFGRHWHSPFGENIYCSARWSFNCDLSRLAGLSLVSS
                     LAVVAFLNTLSNSKDIKIKWPNDILWGNKKLCGSLIEILAESNSSAQVIIGIGLNVNT
                     DTENHPLPDKPWCSLYEIFKQPYDRNFLIASLIVHLEQYLNQFMNTDLDHFMSEWSAV
                     DYLTGKHITVSQASGFISGVACGVNRFGQLILKDDQGIRHYLSSGDTSLQPGKS"
     gene            complement(1648..2385)
                     /locus_tag="I9760_002493"
     CDS             complement(1648..2385)
                     /locus_tag="I9760_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HAT5044249.1"
                     /translation="MIITGFTPLNTQNCILNESRIDLWQFSLDYELHSAYQLLNADER
                     SRADRFYFNRHKRRFTNARATLRIILARYLNTPPERLEFTYNAHGKPNVINSQKLQFN
                     ISHTGEMAILAVGKTYPIGVDIERYSARPYEGIGKNLFSEQEYQELKKAHQSLKPALF
                     FHIWAQKEAFIKASGLGLSYPTKEFHVPTNFPTKQLVDDPLHNMTWQLRSFMPEVCCS
                     GALCYHPSVREIRHGIVQLQQNISLKF"
     gene            2467..3420
                     /locus_tag="I9760_002494"
     CDS             2467..3420
                     /locus_tag="I9760_002494"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HAT5044250.1"
                     /translation="MTRQFLEFEQPIEELNQKIEALRMVGSDNEVNLSEEIARLEAKC
                     SELTENIFSRLEPWQIAQMARHPLRPQTTDYIERIFTDFQELHGDRSYSSAPAIIGGM
                     ARLNGEPVMVLGHQKGKRTKEKVYRNFGMARPEEYRKALRLMRMAEKFKMPVVTFIDT
                     AGAYPGIGAEERNQSEAIARNLFAMSRIKTPIVCIVTGEAGSGGALAIGVGDKIIMLQ
                     FSIYSVISPEGCASILWKDASKASEAARAMGITADRIFENELVDMVVPEPLGGAHRDV
                     DEMASRLKRLLTSELQALKKVPLDQLIELRYQKFMAMGACD"
     gene            3413..4708
                     /gene="tilS"
                     /locus_tag="I9760_002495"
     CDS             3413..4708
                     /gene="tilS"
                     /locus_tag="I9760_002495"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAT5044251.1"
                     /translation="MTRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLS
                     NQLLAVHINHGISGNSLSWQRHCEQLCLDLGIAFIAKAIEFDRSANVEEAARNARYDF
                     FSSLLEDNDCLVLGHHLDDQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFLTC
                     PRVELEHYARIHELKWIEDESNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHC
                     QQAKANLEVLAIKDCPDLLNATDHLLIEPIKGLEFERITNVLKFWLKKNRIQLPSSKT
                     FHRIIHEIIFAKLDAMPTVSWDQIQVRRFQQHLYLLKADQINLPKAIKWQQFPASLTY
                     PDANIELSAVKAQKGLMIPKDAQIEIRFRKGGEEIYWHGQTKHLKKLFQEWQIPPWIR
                     ERVPLVYINDQLACVVGYAVSDLFFTTNPLEAWSIVNNS"
     gene            4969..5505
                     /locus_tag="I9760_002496"
     CDS             4969..5505
                     /locus_tag="I9760_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044252.1"
                     /translation="MISESVIPLAYFITFTCYGTWLHGGKSTSVDYYNNIPGTEFIPY
                     DRRRLYQVKNLMLETPYSLDELRRHIVLQAICEVCGYRQWVLLAAHVRSNHVHLVIHA
                     QPSPERVMNTVKAYASRYLNYTRLDGDRKNRWTRHGSTRYLWNEEEIEATIQYVVYEQ
                     GLPMAVFENKNRVFGVEV"
     gene            5750..6916
                     /locus_tag="I9760_002497"
     CDS             5750..6916
                     /locus_tag="I9760_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 domain-containing protein"
                     /protein_id="HAT5044253.1"
                     /translation="MSDIHCEKSYRSKLLKQGLLGVLILCLVFLFLESFSRWIAATST
                     APFGENIRNDQKVVVSESIRQGIVAIGDSIIARSFYPELFSSLLTKKFSKSYEVYNIG
                     VEGSGILHHRAHLRFLLKRNVKPDLVIMNVSVHNLNLNNFAHDSNDRIIEPAYQDNNS
                     LDYYTRCFIVDKPTIASKMYCKIAEYSYFFRLIRYYHQQLNNSLQAILHTQRLRVDNL
                     FIKYPAVSKQGFSPNFGSLTEQSVNTPRMLEFLRYEYRHVFANFNLGTDGLDSIIDDL
                     NNNSIKVILVLAPVYHPVTRKVYDEFNLPSNGAIVSTLENYALKKNIAFINLFEECQD
                     PVYFQDPVHPNIYGAVKITTLLSQKIINKQFVGPNGYLDKELFQYLKSNNENKA"
     gene            6913..>7670
                     /locus_tag="I9760_002498"
     CDS             6913..>7670
                     /locus_tag="I9760_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HAT5044254.1"
                     /translation="MNFVTFQFVVFLVITVIVFMMMSPYYRKYFILLCSYFFYGTWSV
                     PLISVILFSTSVDYYLGKKIHESHSQTHKKLYLTLSILVNCSILVYFKYMNFFISSID
                     AVGFSLGATQLLLNPMHIILPLGVSFYTFEAISYIVDIYRGEKPASSWLNYNFYIMYF
                     PHLISGPIVRYKELHAQYESKIELPETSRLKEGFQLLLYGFIFKLIIADSMASISDPL
                     FDKYQTIGHLESWLASIAFTIQIYFDFLGYTHIAR"
BASE COUNT         2287 a         1354 c         1548 g         2481 t
ORIGIN      
        1 ttaatatcgt atccatatga ttttaaatag gtatttttat attgtttgag tttattgcag
       61 gaaaaaactt gtttttcctt ttgagtccag tttatttata attgggagtt tttgatatta
      121 gggaatgttg aacagtttta tgatgaattt atgataagga atttattaat ttttcaactc
      181 atttctgcaa aaattaaacg caaatttatc tatgacttaa tattccgtta ataatcagag
      241 tgtacaatgt gatccaagca ataaattttg cttaaatttt gttcatattg tagttgccaa
      301 gaacaccatg gaagcgttct atggagaagt aggtagctgc tatgttaggg agattttagt
      361 catgttgatt ttgactcggc gtataggtga aacattaatc ataggtgatg atgtcaatat
      421 tactgtttta ggtgtaaaag gcaatcaagt tcgcttagga attaatgcac caaaagatgt
      481 ttctgttcat cgcgaagaaa tttatttacg catacaacaa gaaaaagaat ctgatgattc
      541 ggaacaagca gtataaatcg atttccacgc agctcatcca gttccgagta cttaaaaata
      601 ctcgggacga accttaaatc ccttcttgca ccatattgtc aatgtagctt attagctttt
      661 gccaggttgt agggaagtat cgcccgatga aaggtaatga cgaattccct ggtcatcttt
      721 tagaatcaat tgtccgaaac gattcacacc gcaagcaact cctgatatga agcctgaggc
      781 ttgtgaaaca gtaatgtgtt tccctgtcaa gtaatcaaca gcactccatt cgctcataaa
      841 atgatcgaga tcagtattca taaattgatt taaatattgt tccaggtgaa caataagact
      901 agcaattaaa aaattacgat catacggttg tttaaaaatt tcatataaag aacaccatgg
      961 tttatctggt aaagggtgat tttccgtatc tgtattgaca tttaaaccta ttcctatgat
     1021 aacttgcgca ctactattgg attctgccag gatttcaata agactcccgc acaatttttt
     1081 gttaccccag agtatgtcat tgggccattt gattttgata tcttttgaat tgcttaaggt
     1141 atttaaaaag gcaaccacgg ccaaacttga aacaaggctt aaaccagcga gtcgagaaag
     1201 atcacaatta aagctccacc gggcagagca gtaaatattc tctccaaaag gagagtgcca
     1261 gtgtcgtcca aaacgccctc tcccttgagt ttgcatttca gtgcagcaaa tttcgactgc
     1321 tgggcttgag ggcagatctt tcagatagcg gttggttgag tcaattgatg taaataaatg
     1381 caggttaaac ggttggctaa atccttcact gtgtaattgc ttggtaattt cattggagtc
     1441 tagcaggatt aaaggctttt ccaattgata tccttgatgt ggtatgcgag taatgggtat
     1501 acctaaatca ttaagctgat tgatttgttt ccatactgct gagcgactga tttttaaagc
     1561 gtttcctaat tcactcccac tatgacatgc cccatcgccc agtatttgca ttaaagtatg
     1621 ctgagaagta ttaaattgaa acatgattta aaattttaaa ctaatatttt gttgaagctg
     1681 gacgatccca tgacgaattt ctctaacaga agggtgatag cataaagcgc cactgcagca
     1741 tacttccggc atgaaagagc gtaattgcca agtcatgtta tgcaagggat cgtcaaccaa
     1801 ttgcttagta gggaaattgg tgggaacatg aaattctttg gttggatagg agagacctaa
     1861 accactggct ttgatgaacg cttccttttg agcccatata tgaaaaaata aggctggctt
     1921 aagggattga tgtgcttttt tcagttcctg gtattcttgc tcggaaaaca aatttttacc
     1981 aatcccttca taaggtcggg ctgaatatct ttcaatatcc actccaatcg gataggtttt
     2041 acccactgcc aatatggcca tttcacccgt atgactgatg ttaaattgca atttttgcga
     2101 attaatcaca ttgggcttgc catgggcatt gtaagtaaat tccaatcgtt cagggggcgt
     2161 attaaggtat cgggccagga taattcttaa agttgctctg gcgttggtaa agcgtctttt
     2221 atggcgatta aaataaaagc gatcagcacg ggagcgttca tcagcattca ataactgata
     2281 cgcactatgc aattcatagt ctaatgagaa ctgccaaaga tcaatacgtg actcatttaa
     2341 aatgcagttt tgtgtgttta atggagtaaa tcctgtaatt atcatctgtt gtatctgaaa
     2401 tctggcatcg attaacggtt aagagtatca tataccaaag atgtttaatt caccaaagag
     2461 taatcaatga cccgacaatt tttggaattt gaacaaccta ttgaagagtt gaatcaaaaa
     2521 attgaagcac tgcgtatggt tggcagtgat aatgaagtta atttaagtga agagatagct
     2581 cgacttgaag cgaaatgctc cgagttaaca gagaatatat tctccaggct tgaaccatgg
     2641 caaatcgctc agatggccag acatccttta aggccgcaga ctactgacta tattgagcga
     2701 atttttactg attttcagga gttgcatggt gatcgcagtt attcttcagc ccctgcaatt
     2761 atcggaggca tggcgcgatt aaacggggag cctgtcatgg tgcttggtca ccaaaaaggt
     2821 aaacgtacca aggaaaaagt atacagaaac tttggtatgg caagacctga agaatacagg
     2881 aaagcactgc gtttgatgag aatggctgaa aaattcaaaa tgcctgtagt cacatttatt
     2941 gatacggctg gagcttatcc tggcataggt gctgaagaac gtaaccaatc agaagccatt
     3001 gcacgcaatc tttttgctat gtctcgcatt aaaacaccaa ttgtctgtat tgttacagga
     3061 gaagccggtt caggtggtgc cttggcgatt ggggtcggtg ataagattat tatgctgcaa
     3121 ttcagcattt attcagtcat ttctcctgag gggtgcgcct caatcctttg gaaagacgcc
     3181 tctaaagcga gtgaagccgc gcgtgctatg ggaattactg cagacagaat attcgaaaat
     3241 gaattggttg atatggtagt ccctgagcct ttggggggcg cacaccgaga cgttgatgag
     3301 atggcaagtc gcttgaagag attattaacg agtgaactcc aggctttgaa aaaggtgcct
     3361 ctggatcaat taattgagtt aaggtatcaa aagtttatgg caatgggtgc ttgtgactag
     3421 gcctttacta agtcccgaat gggtagctcg ccttaaacaa ttcaagaaat tgatagtagg
     3481 gttcagcggt ggtctggact ctacagtgct cctgcatgtc cttgcctcaa ctcctccctt
     3541 gtctaatcaa cttttggctg tccatattaa ccatggcatt agtggaaatt ctctgagctg
     3601 gcaaaggcat tgtgagcaat tgtgcctgga tttaggtatt gcttttattg ccaaagcgat
     3661 tgaatttgat cgttctgcaa atgttgaaga agcagcacgc aatgcgagat atgatttttt
     3721 ttcctcctta ttagaggata atgattgtct ggttttgggg catcatcttg atgatcaggc
     3781 tgaaacagtt cttttgcaac tgtttcgcgg tgcaggagtt gatggcttgg cagccatgca
     3841 ggaatgcagc aatttgggtg ctgggcaatt ggcaagacca tttttgacct gccccagagt
     3901 ggaattagag cattatgctc gtattcatga attgaaatgg atagaagacg aaagcaatca
     3961 agatacaaaa tattctcgca attatctgcg ccatcgcgta atgccattat tgcttgaaaa
     4021 gtggccggga gtagcaggta atatttcccg agctgcgact cattgtcagc aggcaaaagc
     4081 gaatcttgag gtattggcta taaaggattg tcctgatttg ttaaacgcta ctgatcattt
     4141 gctgattgaa cccataaaag gacttgaatt tgagcggatt acaaatgttt taaagttttg
     4201 gcttaaaaaa aatagaattc aattgccttc gtccaagacg tttcatcgta tcattcatga
     4261 aataattttt gcaaagctcg atgccatgcc aacagtgagt tgggatcaaa tacaagtacg
     4321 tcgtttccaa caacatcttt atttgcttaa ggcagaccag atcaatttac cgaaggctat
     4381 taaatggcaa caatttccag cttctttaac ttatccagat gccaatattg agttgtctgc
     4441 agtaaaggcc caaaagggat taatgatccc gaaagatgcg caaatagaaa tacgattcag
     4501 aaagggtggc gaagagattt attggcatgg ccaaaccaaa catctaaaaa aattatttca
     4561 ggagtggcaa attcccccat ggataaggga gagagttcca ttggtttaca ttaatgatca
     4621 attggcttgc gtagtagggt atgcagtgag cgatttgttt tttactacta atccgctgga
     4681 agcttggtct atagttaata attcttaata tcaacaatct ccgctccctt acggtcgcgg
     4741 ctcggttttg actataattc catttgcact cccttatggt cgtagctcgg ttttagccat
     4801 aattccattt aataatctga ttaatcatct gagccgcgac cgtgaggaag cggaaaactg
     4861 gttgttgtac taattttcca cagaaaaatt tacttcaaaa ataattagtt agtgtgataa
     4921 ccttttctta cataaacaag tcctaaacca aatggaaatg gtgaaataat gatatctgaa
     4981 tcagtcatcc cacttgccta ttttattacc tttacctgtt atggaacatg gttgcatgga
     5041 ggcaaatcaa cttcagttga ttactacaat aatataccag gaactgaatt tatcccttat
     5101 gatcgaaggc ggttatatca ggtaaaaaac ctcatgctgg aaacacctta ttccctggat
     5161 gaattaaggc gtcacattgt tttgcaagca atttgtgaag tgtgcggata tcgtcaatgg
     5221 gtattactgg ctgctcatgt aagatcaaat catgtgcatc ttgttattca tgcccaacca
     5281 tctcctgaac gcgttatgaa tacagtaaaa gcatacgctt cgcgttattt aaattataca
     5341 aggctagatg gtgatagaaa gaacagatgg actcgccatg gaagtactcg ttatctttgg
     5401 aatgaggaag aaattgaagc aacgattcaa tacgttgttt atgagcaagg gttaccaatg
     5461 gctgtgtttg aaaataaaaa cagagtgttt ggagttgaag tttgatctat ccgctctcgg
     5521 ttttagttat aattccattt aatccactcc cttacggtcg tggctcggtt ttagccataa
     5581 ttccatttaa ttatctaatt aatcatccga gccgcgactg tgagggagcg gattacgttt
     5641 aaactaaatt atttcttcag tttttttata gcttttaata ctatacatca ttatcaaata
     5701 tgtttattct gcgcaaattc gaatattgta aaattaattt ctgttattta tgtctgatat
     5761 ccattgcgaa aaaagctaca gatcaaaact tctgaaacag ggtttgcttg gtgtattaat
     5821 tttatgcctg gtttttttgt ttcttgaatc tttttcaaga tggatagcag ctacaagtac
     5881 agcacctttt ggtgagaata tcagaaacga tcagaaagtg gttgttagtg aatctatcag
     5941 gcaaggcatc gttgctattg gggattccat tattgccaga agcttttatc cagaactttt
     6001 ttcttcttta ttgactaaaa aattttccaa atcctatgaa gtatataaca ttggagtaga
     6061 gggttcggga attctccatc atcgggcaca tctaaggttc ttattgaaaa ggaatgtaaa
     6121 accggatctt gtgattatga atgtttctgt tcacaatctt aatttgaata attttgctca
     6181 tgatagtaat gatcgcatta tagaaccagc atatcaggat aataactcgc tggattacta
     6241 tactcgttgt tttatagtgg acaaaccaac aatagcctca aaaatgtact gtaagattgc
     6301 agagtattct tatttttttc gtttaatccg ttattatcat caacaactaa ataatagttt
     6361 acaagccata ttgcatactc agcgcttacg tgttgataat ttattcatta aatatcctgc
     6421 ggtttcaaag caaggttttt cgccgaattt tgggagtctt accgaacaat cagtgaatac
     6481 gcccagaatg cttgaatttt taagatatga atacaggcat gtgtttgcta attttaattt
     6541 gggtaccgat gggttagaca gtattattga tgatctgaat aacaattcaa ttaaagtaat
     6601 tttagtcttg gcaccagttt accatccagt aactcgtaag gtttatgacg aattcaactt
     6661 gccatctaat ggcgctatag tcagtacgct tgaaaattat gctttaaaga aaaatattgc
     6721 ttttataaac ttattcgagg aatgtcagga tcctgtttat ttccaggatc cagtacatcc
     6781 taatatctac ggtgctgtaa agataaccac attgctatca caaaaaatta tcaacaaaca
     6841 atttgtaggg cctaacgggt atttggataa agaattgttt caatacctaa aaagcaataa
     6901 tgagaacaaa gcatgaattt tgtcactttt cagttcgttg tttttctagt tattacggtc
     6961 attgttttta tgatgatgtc tccctattat cggaaatatt ttattttgct ttgcagctat
     7021 tttttttacg gtacctggag cgttccatta atcagtgtca tcttattttc aacctcggtt
     7081 gattactatc tcggaaaaaa aatccatgag agtcattccc aaactcataa aaaactctat
     7141 ttgactttat ccattttggt taattgctcc attctggttt atttcaaata catgaatttt
     7201 tttatttcca gtattgatgc tgttggcttt agtttgggag ccacccaact tttgttaaat
     7261 cctatgcata ttattttgcc tctcggtgtt tctttttaca cttttgaagc gatcagttac
     7321 attgtagata tctatcgagg ggaaaagccg gctagtagct ggttaaacta taatttttat
     7381 attatgtatt tcccgcatct gatttcagga ccgattgtcc gttataaaga actgcatgct
     7441 caatatgaaa gtaaaattga attaccggaa acttctcgtt taaaggaggg gtttcagtta
     7501 ttactttatg gctttatttt caaattgatc attgccgatt caatggcctc aatctcagat
     7561 cccttgtttg ataagtatca gacaattggc catctggaat cctggttggc cagtattgca
     7621 tttaccatac agatttattt tgatttcctt gggtataccc atattgcgcg
//
LOCUS       DACTJE010000159         7583 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.159, whole genome shotgun sequence.
ACCESSION   DACTJE010000159 DACTJE010000000
VERSION     DACTJE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.159"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..521
                     /locus_tag="I9760_002499"
     CDS             <1..521
                     /locus_tag="I9760_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAT5044255.1"
                     /translation="LMLAVTLCTTPLFILFTLFDQTKSFFDRWLGVLAGFAFVLIFVS
                     SVVGLCIHLLHWVTYAFLGNTDELTAAIWVPIFIVACLCVMGITQAVAIGKSIGGSVC
                     TSAGSAMVGGFIGGGLGAWGFSKSSVQKPINTLTQGLGGASRLVEKGKQLGTASHNVF
                     KQLHKNLRRGAS"
     gene            518..1234
                     /locus_tag="I9760_002500"
     CDS             518..1234
                     /locus_tag="I9760_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAT5044256.1"
                     /translation="MSKTNWNDYFKRARSWADDQFGRIEQSRNRYQAAFFSAMGLNVV
                     ALIVIGMLAHYQTVVPMLVHHYDNGVTTVEPMENKLTPINRAQIESDIARYIQYRESY
                     DASSYRAQFELVHLLSNSTVAKEYLQEQDAANTASPIHALGNHIKREVRIYSINFLDS
                     VLANEKDLHKDHHALAEVVFSLIDTDKTSGKTTSTHYNAMISWRYTNPPDSPETRWKN
                     WDGFEVTRYSRQTRLMEAQA"
     gene            1231..1983
                     /gene="virB9"
                     /locus_tag="I9760_002501"
     CDS             1231..1983
                     /gene="virB9"
                     /locus_tag="I9760_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HAT5044257.1"
                     /translation="MKKILLSAAVLLLSLGTYAQNNPTSLPTDIRIKKVVYRENDVIP
                     VHGIPFTTTQIQFAKQERVLDIEGGDTTAWMVTYHPQLENMIFIKPTMFDSKTNMTVI
                     TNQRAYYFSLTSAKKLEQEPGKKTYALKFEYPQPQPVNTKNTTPNKTVHPKVVNTAYR
                     FSGSPQLVPRHVFDDGKFTYFELSAQGSVPAIFAVDDQSGKESTVNTRREGKFIVVQR
                     LAPQFTLRQGGLTASVFNTPEINRIRANRRPK"
     gene            1980..3071
                     /locus_tag="I9760_002502"
     CDS             1980..3071
                     /locus_tag="I9760_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HAT5044258.1"
                     /translation="MSIDDKKREDSLGNRTQVAKNPKHTAIKDKLLLAGVVVICIVIG
                     IGGLFPKRKAHAVSEPDEKQSLSMALNQNLELIAALKENNAKAQSGYKGGDPRRPPLL
                     KTIQTQTVSKETLARMNAPSTFFSSGGNEVISNTTGGEVQASKTLTGRDGNSEFLNQQ
                     NDITSVSAKRLPHPAMTVPAGEMIPATLETAINSELAGMVRAITTRDIFALEGSKLLI
                     PKGSTLVGQFNTAITQGQSRIFVVWNRLQMTNGIIVTLNSPGSDPIGRSGQAADYIDR
                     HFFERFGTGALLSVLGAYTATGGVHGQDEYNSMSQYRMNIASSFQQAANQTLQQDMQT
                     RPTLQINQGAAINVFVAHDLDFYRVAGRA"
     gene            3073..4077
                     /gene="virB11"
                     /locus_tag="I9760_002503"
     CDS             3073..4077
                     /gene="virB11"
                     /locus_tag="I9760_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HAT5044259.1"
                     /translation="MEAINACFSPGATGLLSPLQDFLEDEHVSEILINKPKEVFIERH
                     GQLTRFDIPVLTSQYLRRLFLLIANENKQTLSESTPVLSGNLADGSRVQLVIPPASLY
                     ETLSIRKFSLKQVSFDEYKTKGFFSSARGVGIKEHSECLDKSDELLELYDTKNWQEFI
                     RQAIVSKKNIIISGGTSSGKTTFLNSCVGQIPLHERLITLEDTYEMDVPHTNIVRLKA
                     LKQVGEQTQKITMQDLVQATLRLRPDRIIMGEIRGRELFDFVSACSTGHSGALATIHA
                     NNPKVAFMRMAQLYKLNQVAGMDEADIYKVLHEVIDVIVQLQKTPDGRRLVEVYYKQA
                     "
     gene            4130..5971
                     /locus_tag="I9760_002504"
     CDS             4130..5971
                     /locus_tag="I9760_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAT5044260.1"
                     /translation="MAVLLTGIAEWASPLVLVDLLTRGFYWETAIASGITALLMSCVV
                     GFLKQGFYRYLSFLISSILLGGMVVFLLGFELYSWIFLSSVQSLSGLMELAFLRYQEP
                     ELWHLFLTVEGIVFILTFLAIALYLFHWLKPNNKALGNAHFSNGFETKKAGFFERQDQ
                     SIVIGKKYGAPLYSNGFEHVLVFAPTGSGKTRSIGIPNLFNYPYSVVCNDVKLTLFRT
                     TSGYRERVLGHRCFCWAPADENRITHCYNPLSLISEDKIQRLTDIQRIAHILMPDNKK
                     SDPIWQQASRKLFKVAVLYLLDTPERPTTLGEINRLVKQAGFDDWLASVLEETNHLDP
                     EFYRNGYSYLNNHEKTRSSILETFSGYFELFDDPTIDAATARSDFDLRQLRREKITIY
                     IGFTDDDMERLSPLLTLFWQQLISVMIKNIPDPVDEPYPLLCLIDEFSSLGRIERLRR
                     SLKLLREYRVRCVLIMQYIAQTYEQYTHDEAKAFTNIKTKIAFATEDIFDAEYVSKLL
                     GTRTVKVSAGSTSTQTQGYSESKSYNYQAIPLLRPDEVMRLPEDQTLIMRTGHAPVKA
                     EQMVWYLDTTMKHLACGSTEVPRQNVTHHPFVHQASDKSLMPEALEL"
     gene            6069..6866
                     /locus_tag="I9760_002505"
     CDS             6069..6866
                     /locus_tag="I9760_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HAT5044261.1"
                     /translation="MNHLKIIGTAGLFMMSCAAQANDKELWAHIEALYSKIGTLQTQI
                     NSIPQGAPGPMGPAGPKGERGPAGTYTAGEGISISNGEIKTTLTHHIGEEYNGGIIFW
                     VDKTGQHGLIASKTDLNNNEGIQWRNGESGNKVTNARADGIYAGESNTKLIIAQQTID
                     NQSGTFAALLAANYRVLGDGITPCPNPTTESPTCYGGWYLPSAYELTLLHNNLQRQGI
                     STFAPDYYWSSTESSVGEAWLQNFSTGELMASGKASTLGHVRAVSRF"
     gene            6955..>7583
                     /locus_tag="I9760_002506"
     CDS             6955..>7583
                     /locus_tag="I9760_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044262.1"
                     /translation="MEDIQRNRIIAFVDILGFKTLINSAEKNKVIDFLYELRNIDSRF
                     RENFINRETLNGYIVVTKIHPDISSYSDHLLISAPINLDSLQSITTSLAYISYTIIDI
                     QLLALKRGFALRGAIAHGEIFYDEEKKIFAGQALIEAIEGESCVAKYPRVIICKSVKD
                     LLEIKKLEKIDFSFLESDFDGVKFLNFLDAKYISQKFKDSIENELNLFN"
BASE COUNT         2259 a         1649 c         1657 g         2018 t
ORIGIN      
        1 aactgatgtt agccgtgacc ttgtgcacta cacctctgtt catcctgttt accttgtttg
       61 accaaaccaa atcttttttt gaccggtggc ttggcgtgct cgccggattt gcctttgtgc
      121 ttatttttgt atccagtgtg gtaggccttt gcatccactt attgcattgg gtgacttatg
      181 cttttttggg caatacggat gagctaactg cggccatttg ggtgccgatt tttatcgtgg
      241 cctgtttatg tgtcatgggc atcacccaag ccgtggctat tggtaaaagc attggtggtt
      301 cggtttgcac atcagcaggt tcagccatgg tgggaggctt tattggtgga ggcttagggg
      361 catggggatt ttccaagtct tccgttcaaa aaccaataaa tacgctgact caaggattag
      421 gaggcgcatc gcgtctcgtg gaaaaaggca aacaactggg tacagccagt cataacgtat
      481 tcaaacaact gcataaaaac ctgcgaagag gagcatcatg agtaaaacca attggaatga
      541 ttattttaag cgtgctcgaa gttgggcaga cgaccagttt ggccgaatcg aacaatcgcg
      601 aaaccgctat caagccgcat ttttctcagc catgggcttg aatgtggtag ctctcatcgt
      661 gataggcatg ctcgcacatt atcaaaccgt agtgccgatg ctggttcatc attacgacaa
      721 tggggttact accgttgaac ccatggaaaa taaactaaca ccgataaatc gcgcacagat
      781 tgaaagcgac atcgcccgtt acattcaata ccgtgagtct tatgatgcat caagctatcg
      841 cgctcaattt gaattggtgc acttgttatc caatagcacg gttgccaagg aatatttgca
      901 agagcaagat gctgcaaata ctgcctcacc cattcatgcc ttggggaatc atatcaagcg
      961 agaggttcgc atttacagca ttaacttcct tgattccgtc ttggcgaatg aaaaagacct
     1021 tcataaagac caccacgcat tggcagaagt ggtattcagt ttgattgata ccgacaaaac
     1081 cagcggaaaa accacctcta cgcattacaa cgccatgatt tcatggcgat acaccaatcc
     1141 cccggattcc cctgaaaccc gctggaaaaa ctgggatgga tttgaagtca cgcgctactc
     1201 aaggcaaacc cgtttaatgg aggctcaagc atgaaaaaaa tactgttgag tgcggcagtc
     1261 ctgctcttgt cattaggcac ctatgcccaa aataacccaa cgtccttacc aaccgacatc
     1321 cgaatcaaga aagtggttta tcgggaaaac gatgtgattc cagtgcacgg cattcccttt
     1381 accaccacgc aaattcaatt tgcgaagcaa gagcgagtac ttgatattga aggcggtgac
     1441 accaccgcat ggatggtgac ctatcatcca caactggaaa atatgatttt tataaaaccc
     1501 acaatgtttg attccaaaac taatatgacg gtgattacca atcaacgcgc ctactatttt
     1561 agcctgacca gcgctaaaaa actggagcag gagccaggta aaaaaaccta tgccttgaaa
     1621 tttgaatacc cccaaccaca acccgttaac acaaaaaata cgactcccaa taaaacggtg
     1681 catccaaaag tggtcaacac cgcttatcgg tttagtggca gtccgcagct tgttcctcgt
     1741 catgtatttg atgacggcaa gttcacctat tttgagctaa gtgctcaggg cagcgttcca
     1801 gcgatttttg cggtggatga ccaaagcgga aaggaatcaa ccgtcaatac aaggcgcgaa
     1861 gggaaattca ttgttgtaca gcgtttagct ccgcaattta ctttaagaca aggtggcctt
     1921 accgcttctg tttttaatac accggaaatc aatcgaatca gagccaacag gaggccaaaa
     1981 tgagcattga tgataaaaag cgggaagact ctttgggcaa tcgaactcag gttgccaaaa
     2041 atccaaagca cacagcaatc aaagacaagt tattgctcgc aggcgttgtg gtgatttgta
     2101 ttgtcatcgg tattggcggg ttattcccta aacgaaaagc acatgcagtg agtgagcccg
     2161 atgaaaaaca aagtttaagc atggccttaa accaaaatct ggagttgatt gcagccctta
     2221 aggagaacaa tgccaaagcg caatcgggtt ataaaggtgg cgacccaagg cgtcccccct
     2281 tactaaaaac catacaaacc caaacggtca gcaaggaaac tctagcccga atgaatgcac
     2341 cctccacctt ttttagttca ggcggtaatg aggtgataag taatacaacg ggtggcgaag
     2401 ttcaagccag caaaaccctg acaggccgtg atggaaattc tgagtttttg aatcagcaaa
     2461 atgatattac atcggtttct gcaaaacgat taccgcatcc ggccatgacc gtacctgcag
     2521 gagaaatgat tccagcaacg ctcgaaaccg ccattaattc ggagttggcg ggtatggtac
     2581 gagcaattac aacaagggat atctttgcct tggaaggcag taaattacta atccccaaag
     2641 gctccacgct agtcggacaa tttaataccg caattaccca aggccaaagc cgaatctttg
     2701 tcgtatggaa tcgtttgcag atgaccaatg gcatcatcgt gaccttaaat agccccggca
     2761 gtgaccctat tggtcgttca ggacaagcgg ctgattacat tgaccgccat ttcttcgagc
     2821 gtttcggcac cggtgcgctt ttgtctgttt tgggcgcgta taccgcaaca ggtggcgttc
     2881 atggtcagga cgaatacaac tccatgtcac agtaccgcat gaacattgcg agcagtttcc
     2941 aacaagcggc aaatcaaact ctacagcaag atatgcaaac acgcccgact ttgcaaatca
     3001 atcagggagc tgccattaac gtgtttgtag cacacgacct tgatttctac cgtgtggctg
     3061 ggagggcata atatggaagc catcaacgcc tgcttctcgc ccggcgcaac aggactttta
     3121 tctccattgc aggattttct ggaagatgag cacgtttctg agattttaat caataagccc
     3181 aaagaggttt ttatcgagcg acacgggcaa ttgactcgtt ttgatattcc ggtattaacg
     3241 tctcaatacc tgcggcgttt gtttttactc atagccaatg aaaacaaaca aactttatcg
     3301 gagtcaacgc ccgtgctttc tggaaatctg gccgatggct caagggtaca actggtgatt
     3361 ccaccagctt ccctatatga aacactgtcc atccgaaaat ttagcttaaa acaggtcagc
     3421 ttcgacgaat acaaaaccaa gggctttttt tcatcggcgc gtggggttgg aattaaggag
     3481 cattccgagt gccttgataa atccgatgag ctgcttgaac tctacgatac gaaaaactgg
     3541 caggaattta taaggcaagc tattgtgagt aaaaaaaaca ttatcatatc gggtggaacc
     3601 tcttcgggta aaacgacctt tttaaacagt tgtgtcggac aaataccact tcatgaacgc
     3661 ctcattaccc ttgaagatac gtatgaaatg gatgtccccc acaccaatat cgttcgtcta
     3721 aaagcattaa aacaagttgg agagcaaaca caaaaaatca ccatgcagga tttggttcaa
     3781 gccactttaa gactcagacc cgaccgcatc atcatgggcg aaattcgtgg gcgagaactc
     3841 tttgattttg tatcagcctg ttccacaggg catagcggtg cactggctac catccatgcc
     3901 aataacccaa aagtggcctt catgcgcatg gcgcaactct acaagcttaa tcaggttgca
     3961 ggcatggatg aagctgatat ttacaaggtg ttacatgaag tcattgatgt catcgtccag
     4021 ttacaaaaaa cgcctgacgg caggcgtttg gtggaggtgt attataaaca ggcgtaatcc
     4081 atgggttgtt accattacct tgataggtgg gcttgctata accggccaaa tggcagtgct
     4141 gttaactggg atcgcagaat gggcaagtcc gttggtattg gttgatttat taacacgggg
     4201 attttattgg gaaacagcca tagcaagtgg tatcactgcg cttttgatga gttgtgtggt
     4261 tggctttttg aaacagggat tttaccgata cctttcgttt ctaatcagca gcattctgct
     4321 gggtggaatg gttgtgtttc tactgggatt tgagctttat agctggattt ttttaagttc
     4381 tgtacaaagc ctctcaggac tcatggagct tgccttcctt cgttatcaag aacctgagct
     4441 gtggcatctt ttcctgacgg tcgaggggat tgtgtttatc ctgacgtttt tggctattgc
     4501 gctttatctg tttcattggc ttaagcccaa caataaggca ctcggtaacg cgcatttttc
     4561 aaatggtttt gaaactaaaa aggctggatt tttcgagcgt caggatcaaa gcatcgttat
     4621 tggtaaaaaa tacggtgcgc cactttactc caacggcttt gaacatgttt tggtttttgc
     4681 gcccacggga agcggtaaaa cacgaagtat cggcataccc aatcttttta attaccccta
     4741 ttcggtggtg tgtaatgatg taaagctcac gctgtttagg accacatcag gctaccgtga
     4801 gcgagtatta ggacatcgat gcttttgctg ggcacctgcc gatgagaacc gaatcaccca
     4861 ctgctacaat ccattgtcct taatttccga ggacaaaatt caacgcctga ccgatattca
     4921 gcgcatagcc catattttaa tgccagacaa taaaaaatcc gaccccattt ggcagcaagc
     4981 ctcacgtaag ttatttaaag tggctgtgtt gtacctgctg gacacacctg aaaggcccac
     5041 cacgcttggc gaaataaatc gcttggtcaa acaggctgga tttgatgatt ggctggcttc
     5101 cgtcttggaa gaaaccaatc atctcgaccc agagttttat cgcaacggat actcttatct
     5161 gaataatcat gaaaaaacga gaagctcaat tcttgaaacc ttttcgggat attttgagtt
     5221 attcgatgat ccgaccattg acgcagccac agcacgttct gattttgatc tgcgccaatt
     5281 aagacgagaa aaaataacga tttacatagg ctttaccgat gatgatatgg aacggctctc
     5341 accattgcta acactgtttt ggcagcagct tatttctgtc atgattaaaa acatccccga
     5401 cccagtggat gaaccctatc ctctgttatg cctgattgat gagttttcat cattaggccg
     5461 tattgaaagg ctaaggcgtt cgcttaaatt attgcgcgaa taccgtgtgc gttgtgtact
     5521 catcatgcaa tacatagccc aaacctatga acaatacacc catgatgaag ccaaagcctt
     5581 taccaacatt aaaactaaaa tagcttttgc taccgaagac atctttgatg cggagtatgt
     5641 cagtaaattg ttaggtacac gcaccgtgaa agtatccgca ggctccacca gtacccaaac
     5701 ccaaggttat tccgagtcta aaagctataa ctatcaggcc atccctttat taagaccgga
     5761 tgaagtcatg cgtttacccg aagatcaaac cctgattatg cgaacaggcc atgcgccagt
     5821 aaaagcagaa caaatggttt ggtatttgga taccacaatg aagcatttag cctgcggcag
     5881 taccgaagta ccgcgccaaa atgttacgca tcatccattt gtccatcagg catcggataa
     5941 atcgctgatg cctgaagcac tcgaactata aaccacacag gaggaaacaa agccagtaac
     6001 aaaatcacaa aggatgctct ttaaagagat tatggattca tacagactta attgatgtga
     6061 gaatcaaaat gaatcatctt aaaattatag gtacagcagg gcttttcatg atgtcatgtg
     6121 cagctcaggc aaatgacaag gaactttggg ctcatattga ggcgttatac agtaaaattg
     6181 gtactttgca aacgcaaatt aactcgatac cccaaggtgc acccggtcca atgggtccag
     6241 caggaccaaa aggggagcga ggacctgccg gaacctatac agcaggagaa ggcatttcga
     6301 tcagcaatgg tgaaataaaa acgactctaa cccaccacat tggcgaagaa tataacgggg
     6361 gcattatctt ctgggtcgat aaaacaggac agcatggatt aattgcttcc aaaaccgacc
     6421 tcaataacaa tgaagggata caatggcgaa acggcgaaag tggcaataaa gtaaccaatg
     6481 cccgcgctga tgggatttac gcaggcgaat ccaataccaa actcatcatt gcccaacaaa
     6541 ccatagacaa ccaatcaggt acctttgccg cacttctggc tgcaaattat cgcgtgctag
     6601 gcgatggcat aacgccttgc ccaaacccaa ctacagaatc acccacttgt tacgggggat
     6661 ggtatttgcc ttcagcctat gaattgacct tgcttcacaa taatctgcaa cgtcagggca
     6721 ttagtacttt tgcaccggac tattattgga gttcgactga atccagtgtg ggcgaagcat
     6781 ggttgcaaaa tttttctacc ggggaattaa tggccagcgg gaaagctagt actttggggc
     6841 atgttagggc tgtgagtcgt ttttaagttc aaggattaac tactcattta ttaatgggta
     6901 gttaatgaca acaactatac ttatgttaga tgataaaggt tatataggat tagtatggaa
     6961 gatattcaaa gaaatagaat aatagctttt gttgatattt taggttttaa aacacttatt
     7021 aatagcgctg aaaaaaataa agttatagat tttctatatg agctaagaaa catagattct
     7081 agatttagag aaaatttcat caatcgcgaa actctaaacg ggtatattgt tgtaactaaa
     7141 atacaccccg atatttcaag ttactctgat catttactaa tttctgcacc aattaattta
     7201 gattcattac aaagtataac aacctctttg gcttatatct catatacaat catagatata
     7261 caactcttag cattaaaacg gggttttgct cttagaggtg caatagctca cggcgaaatt
     7321 ttttacgatg aagaaaaaaa aatatttgct ggtcaggcat taattgaagc tattgaaggt
     7381 gaaagttgtg ttgctaaata tcctagagta attatttgta aaagcgtaaa agatttactc
     7441 gaaattaaaa aattagaaaa aattgatttt agctttttag aaagtgactt tgatggggtt
     7501 aagtttttga attttcttga tgctaaatat ataagtcaaa aatttaaaga ttccattgaa
     7561 aatgagctga atctttttaa cga
//
LOCUS       DACTJE010000160         7315 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.161, whole genome shotgun sequence.
ACCESSION   DACTJE010000160 DACTJE010000000
VERSION     DACTJE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.161"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..440)
                     /locus_tag="I9760_002507"
     CDS             complement(<1..440)
                     /locus_tag="I9760_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HAT5044263.1"
                     /translation="MLPVFKKYLASKHKDSLEYEFYKTVVEQGVKTNFGCCFAIWDGE
                     ENDHMWQVFTPKHDNYGVIIIINSGDLYNAVNQVENKHIYLSKVKYLSDDKAKSMKPE
                     ECSHSNKRSFHFEESHFLKRIAYKNEKEVRAIISSVDNNWTVLL"
     gene            complement(695..847)
                     /locus_tag="I9760_002508"
                     /pseudo
     CDS             complement(695..847)
                     /locus_tag="I9760_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633641.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(940..1182)
                     /locus_tag="I9760_002509"
     CDS             complement(940..1182)
                     /locus_tag="I9760_002509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044264.1"
                     /translation="MKEFLMGCIPGIIIILIIALVNLTPKPSFSDPAWEIVPTPSSTS
                     DIIRTRVPNGWLVTYRAYLARGLVYIPDDKHEWKVK"
     gene            complement(1195..3171)
                     /locus_tag="I9760_002510"
     CDS             complement(1195..3171)
                     /locus_tag="I9760_002510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HAT5044265.1"
                     /translation="MNNYITKKEQEITMFSNRGSGLKPKTCNQKLTLHHAQEVAKNKN
                     GKCLSEKYKNVDTKLKWECEFGHQWEATLYNVRKIGSWCPECAGVAKLTLDEMKQLAE
                     RKKGKCLSNAYINSDSKLTWECEFGHQWDALPNSIKNKKSWCPECAERKKLTISQMKE
                     LAISKGGKCLSASYKNNRSKLKWECEFGHQWNASPSSILNGNSWCPDCAGNVKQTIEA
                     MRELARNRNGECLSVEYTNSHSKLTWQCHLGHQWEATPSDIKNGKWCRHCYKSKRKGE
                     NAIRCFFEKIFNKKFPSSRPLWLMYEGGRLELDGYCEELQIAFEYQGQYHFDKTAYWY
                     PDKSISFERRQLIDEYKRKTCEENKVLLIEINELNPRSNPDKWKDTIKRILISKDIAI
                     PSNYDAIPFGAVEYHSSGNYTQEAYEIAKLNLGMCHSENILYKEQLVEWECHLGHRWS
                     ASLSQVILRNTWCPSCARRKKLTIKQMQALAKLKGGKCLSTEYINARTELLWECSEGH
                     RWLAIPSSIKNNDSWCLECSGSKKLTIDNMKELAKKRNGECLSDQYNGNKVNLIWKCN
                     LGHIWQATPSSIKNRGAWCPVCGGSNKLTIEQMQELAKLKGGKCLSTVYVNARTKLLW
                     ECSAGHQWLAIPDKIKNIGRWCKECKKSKSTIIS"
     gene            complement(3164..3622)
                     /locus_tag="I9760_002511"
     CDS             complement(3164..3622)
                     /locus_tag="I9760_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044266.1"
                     /translation="MSPEKIKTTVTCELKSRAKIIKENYHNPELTLGNVLEALAQYYG
                     FKDWNTAFAKLKDTPYNSSAYPAENSERLDRIINKILDGRRNKCDMSYRRNHNYITFL
                     KKYIDKGLETWAPINWDDFVLKINLKFSDDYVFSVNRMLANVYHKGLFNE"
     gene            complement(3895..4644)
                     /locus_tag="I9760_002512"
     CDS             complement(3895..4644)
                     /locus_tag="I9760_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011789342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HAT5044267.1"
                     /translation="MLRLMLTDEHWSKLRTIIREHGIYDKPNLRKTVEGILYRMRTGL
                     PWRDLPTFFGVWISIYQQFNRWASKNKLMAIFKTLVQDPDLEWEFIDGSIVKAHQHST
                     GAASSDDEAIGKSVAGNTTKIHMAADAHGLTIDFIVTGGEVHNCKVAPQFITQLPSAD
                     YTIADKGYDKEDLRHIIRQKSSQPVIPRKSNSTIGNDDMDWALYKYRHLVENIFARIK
                     HFRAIATRYDKLKRNYASMVDLACGLMWLPM"
     gene            complement(4895..4971)
                     /locus_tag="I9760_002513"
     tRNA            complement(4895..4971)
                     /locus_tag="I9760_002513"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4935..4937),aa:Arg,seq:cct)
     gene            5303..6367
                     /locus_tag="I9760_002514"
     CDS             5303..6367
                     /locus_tag="I9760_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1273 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044268.1"
                     /translation="MKIFKGLKKNTGFRKQSNTQKISNSSIQSSITSLSGDASQPRFD
                     FFPINNRDVLLKSPSFNHIGSEFQPMVSKRDVNSISITLPIKHNVHRNHNSNLPVDSI
                     SVVDSSNQQDHISYLHICFTHEEGKWYLFLKCFTTPDVFNYDFAPLLKILHKPDIRTY
                     PMSFGYPGVIDRTELIDKDRKIYARQFIIHGPNDEAKILQDVHDFNLNCLRRFEELTY
                     GRDNSAYRNSQALGRILHKIVTAYQEVTVNEYDIALNRLKIVFQDQVFQNDPQICLSL
                     THIVNALIVLSQKKELNNKGIEMDEHQLHLILKELAKHEGKKFKNNLWFPVGTVETIS
                     QEVDELFDLKQTTAAASIMS"
     gene            6415..6870
                     /locus_tag="I9760_002515"
     CDS             6415..6870
                     /locus_tag="I9760_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HAT5044269.1"
                     /translation="MKQIKGILLSIISLSFLPFLVFASDASISLVGVQGYDLVSYHQK
                     NGPVRGSGNHTAYHNGVAYLFATDENKKVFQANPEKYLPAYGGYCAFAVSVGRKVVSD
                     PLAWKIVDGILYLNLNKQVQEFWSKDIPGNIKKGNIQWEKIKDIDPQKI"
     gene            complement(6948..7315)
                     /locus_tag="I9760_002516"
                     /pseudo
     CDS             complement(6948..7315)
                     /locus_tag="I9760_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213588.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
BASE COUNT         2239 a         1359 c         1191 g         2526 t
ORIGIN      
        1 tgaagcaaca cagtccaatt attatctaca gaactaataa tagctctcac ttctttttca
       61 tttttataag ctattcgctt taaaaaatga ctttcttcaa aatgaaatga ccttttattt
      121 gaatgagaac attcttctgg tttcattgat ttcgctttgt catcacttaa atactttact
      181 ttagatagat atatgtgctt attttctact tggttaaccg cattatacaa atcaccacta
      241 tttataataa tgatgactcc ataattatca tgtttcggtg taaagacttg ccacatatga
      301 tcattttctt caccatccca aatagcaaaa cagcaaccaa aatttgtttt aacaccctgc
      361 tcaaccactg ttttatagaa ttcgtactct aagctatcct tgtgtttcga tgctaaatat
      421 tttttaaaaa ctggcaacat ttctccttca cagggatcta ttgattccca cttcaacact
      481 ctgttaaacc atagtttctc cgtattcaga taatctaaaa agtagttagt cttaatgtac
      541 tttcataatg actggtctgt atttaaacta cctacataag gattgatact aaatcctgta
      601 tgaattttat tcataatttg gagaatccta aaaaattacc ttctttatca aaaatacttc
      661 ttgaattaaa tttcacctac tcctaattca tccttcatgc aaaaagctct tcaattgttg
      721 tttgcatctc ttcttgacta ggtttcgcat agcgaaatat ttctttaata gagacattac
      781 cacttaatac tgacccaaaa tacactccat gcatattagt aagcttttca aagaaatatg
      841 acctaatcta tggagtttaa taactgtata tattttaaaa tgggagtaga aagcacgaat
      901 cgtaaaattg gagtgtcgtg cagtcctagt tttaatattt tatttaactt tccactcatg
      961 tttatcatca ggaatatata ccaatcctct tgctaaataa gctctatacg taactaacca
     1021 gccattcggt actcgcgttc taataatatc tgaagttgaa cttggagttg gaacaatttc
     1081 ccaagctgga tctgaaaatg atggtttggg agtcaagttt accaatgcaa tgattaaaat
     1141 tatgattatc ccagggatgc aacccattag aaattctttc atgagaactc caagttaaga
     1201 aattattgtt gatttgcttt ttttgcattc tttgcaccat cttcctatat ttttaatttt
     1261 atctgggatt gctaaccatt gatgtcctgc actacattcc catagaagct tggttctagc
     1321 atttacatac acagtagata agcatttccc tcctttgagt tttgccagtt cttgcatttg
     1381 ctcaattgtt aatttattgc ttcctccaca tacaggacac catgcaccac gatttttaat
     1441 gctagatgga gtcgcttgcc aaatatgacc taagttacac ttccaaatta aattaacctt
     1501 atttccatta tattgatctg ataagcattc cccatttctt tttttagcta attctttcat
     1561 attgtctatg gttagttttt tactaccaga gcattctaaa caccatgagt cattattttt
     1621 aatactagat ggtatggcta gccatcgatg tccttcactg cattcccata gaagttcggt
     1681 tctggcattt atatactcag tagataagca tttcccacct ttgagttttg caagcgcttg
     1741 catttgctta atagttaact tttttcttct agcgcaagac ggacaccagg tatttcttaa
     1801 aataacctga gataagctcg ctgaccagcg atggcctaga tggcactccc attctacaag
     1861 ctgctctttg tagagaatat tttcactatg acacattcct aggtttaatt tagctatttc
     1921 ataggcttcc tgcgtgtaat tacctgagga atgatactca acagcaccaa aagggatagc
     1981 atcataattt gagggtattg caatgtcttt agatatcaat attctcttta tggtatcttt
     2041 ccatttgtca ggattgcttc tgggattcag ttcatttatc tctattaata agactttatt
     2101 ttcttcacat gtttttcttt tgtattcatc tattaactgt cgtctttcga agcttatact
     2161 tttatctgga taccaatatg cggttttatc gaaatggtat tgaccttgat actcaaaagc
     2221 tatttgaagc tcttcgcaat aaccatctaa ttctaacctc ccgccttcat acataagcca
     2281 caaaggtctt gaggaaggga attttttatt gaatattttt tcaaaaaaac accgtatcgc
     2341 attttcgcct ttacgtttac ttttataaca gtgacgacac cattttccat tttttatatc
     2401 acttggggta gcttcccatt gatgccctaa gtgacattgc caagttaatt tactgtggct
     2461 gttcgtatat tctacagaca aacattcgcc atttctattt ctagctaatt ctctcattgc
     2521 ttctattgtt tgttttacat taccagcaca gtcagggcac cacgaattac catttagaat
     2581 acttgatggc gaggcattcc attgatgacc aaattcacat tcccatttta atttactacg
     2641 attattctta tatgaagcag ataagcattt cccccctttg cttatagcta attctttcat
     2701 ttgactaata gttaattttt ttctttctgc gcattcgggg caccatgatt tcttgttttt
     2761 aatcgagttt ggcaatgcat cccattgatg accaaattcg cattcccatg tcaattttga
     2821 atctgaatta atataagcat tcgaaagaca tttccctttt tttctctctg ctagctgttt
     2881 catctcatca agtgttaatt tggcaacacc cgcacattca ggacaccaag atccgatctt
     2941 tcttacatta taaagtgtag cctcccattg atgaccaaac tcgcattccc attttaattt
     3001 tgtatcaaca tttttatact tttctgaaag acacttccca tttttgtttt tggcaacttc
     3061 ctgggcatga tgtaatgtta gtttttgatt gcatgtctta ggcttgagcc ctgaacccct
     3121 atttgaaaac atggtgattt cttgctcttt ctttgtgatg taattattca ttaaagagcc
     3181 ccttgtgata tacatttgcc agcattctat tgacactaaa aacataatca tccgaaaatt
     3241 ttaagttgat ttttaaaaca aaatcgtccc agttaatggg ggcccacgtc tctagtcctt
     3301 tatcaatata ttttttaaga aatgttatgt agttgtggtt tcttctataa gacatatcac
     3361 atttgttcct acgtccatca agaatcttat tgataattct atccaagcgc tcagagtttt
     3421 ctgcaggata tgctgaggaa ttgtaaggtg tgtcttttag ctttgcaaac gcggtattcc
     3481 aatctttgaa accataatac tgtgcaaggg cttctagaac attacctaat gttaattctg
     3541 gattatgata attctctttt attatttttg cgcgtgactt gagttcgcat gtaactgtag
     3601 ttttaatttt ttcaggtgac attgattgac tccaattata tatcaagttt attcagagcc
     3661 tgtattactg aaacttaata aaaagagtga aaccaatatt tagataacta gagctattca
     3721 ccatatttac aggcggcaag ctagccctat gctcggcgaa cattctctat ataataagca
     3781 tacgtgtcaa gttaaaattt taattttgtc gcggattaat ggcttgtcca gaatctgtag
     3841 caaaagataa caaaattata ataatttaaa gttgaactag ggcctgttta caattcacat
     3901 tggtaaccac attaacccac acgctaagtc gaccatagag gcataatttc tttttaattt
     3961 atcgtacctt gtcgctattg cacgaaaatg ttttattctt gcgaagatat tttcaaccag
     4021 atgccggtac ttgtataaag cccaatccat atcatcattt cctatcgttg aattactttt
     4081 tcttgggata acaggttgcg atgatttctg cctgatgata tggcgcaaat cctccttgtc
     4141 atatccctta tcagcaatcg tataatcagc agatggtagc tgagtgatga attgaggtgc
     4201 aactttgcaa ttatgtactt caccaccggt gactatgaaa tcaattgtca aaccatgtgc
     4261 atcagccgcc atatgtattt ttgttgtatt cccagctaca gatttcccaa tagcttcatc
     4321 atccgaacta gcagcacctg tactatgctg atgagccttt acaatgcttc catcaataaa
     4381 ttcccactca agatctgggt cctgaacaag ggttttaaaa atagccatta gtttgttttt
     4441 cgaggcccaa cggttaaatt gttggtaaat tgaaatccat acaccaaaga aagttggtag
     4501 gtctcgccaa ggcaatcctg ttcgcatacg gtataaaatc ccttcgactg tttttcgcaa
     4561 attgggtttg tcataaattc cgtgttcgcg aatgatcgtt cttagctttg accaatgctc
     4621 atctgtgagc ataagcctta gcattgcaaa ctcgtttttt gctgattgtg agaatcgcaa
     4681 gtttacgagt ttgttcttat aaatctatcg attacagcga attgtaaaca ggccctaatt
     4741 tagaacttaa taacaaactt cagggattta ttgagtctct aaatgcgtat atttattaga
     4801 tataagtatt agcagacact tttaatttgc accctgagtg caccctcaaa agttagtatg
     4861 gacaaaagta aatttcgcaa ctcattgatt tcattggcgt cccgggcagg attcgaacct
     4921 gcgacctgcc ccttaggagg gggctgcgct atccatctgt gccaccggga ctttgacttt
     4981 atttatcaaa cgcaagtcta cccttatagc cagaaaatga caacggggaa gcaaacttct
     5041 tctatgaaaa tgtaaagatt attgattggg ctggttggat tggaatgtgt tcaactattc
     5101 tgtcatttct taattccgga tattgataag ttaattttta ttaaactgag tgaaagttct
     5161 tgtggttggc catcaagatg gttataacct gataacagca tgacatgaaa gaatatctat
     5221 tgaccaaata ttaataatta agataccttg cgctagttga taatggattg aaaatttgag
     5281 tcattaaagg agatgttaaa atatgaaaat ttttaaagga ttaaagaaaa acactggttt
     5341 taggaagcag tcaaatactc aaaaaatatc taattcatcg attcaatcat ccattacttc
     5401 tttatctggt gatgcaagcc aaccccgatt tgattttttt ccaataaaca atagagatgt
     5461 tttattaaaa agcccctctt tcaatcatat cggttcagaa tttcaaccca tggtatccaa
     5521 gagggatgta aactcaatct cgattacatt accaattaaa cacaatgttc atcgaaatca
     5581 taatagtaat ttgccagttg actcaatatc agtcgtggac tcatctaatc aacaggatca
     5641 tatttcctat ttacatatat gctttactca tgaggaagga aaatggtatt tatttttaaa
     5701 gtgtttcaca acgccggatg tttttaatta tgattttgca ccattattga aaatactaca
     5761 taaaccagac ataagaactt atcccatgag ttttggttac cctggtgtta ttgacaggac
     5821 agagctcatt gacaaggata gaaaaattta tgctaggcag tttattatac atggccctaa
     5881 tgatgaggct aaaatcttgc aagatgttca tgatttcaat ttgaattgtt tgaggcgttt
     5941 tgaagagtta acctatggta gagataatag tgcttataga aactctcaag cgctagggag
     6001 aattttgcat aaaattgtaa ctgcttatca agaagtaacg gtaaatgaat atgatatagc
     6061 attgaatagg cttaagattg tatttcaaga tcaagtattt caaaatgatc cacaaatttg
     6121 tttgtctttg actcacatag tcaatgcatt gattgtttta agtcaaaaaa aggaacttaa
     6181 taacaaggga attgaaatgg atgaacacca attacatctc attttaaaag aacttgcaaa
     6241 acatgaaggt aaaaaattta aaaataatct atggtttcct gtagggactg ttgagacaat
     6301 tagccaagag gtagacgaac tgtttgattt aaagcaaact acagccgcag catctataat
     6361 gagttaatag tgatttaagc actaccagtt acaatttaat taaggacgat aaaaatgaaa
     6421 caaattaaag gaatactatt atcaataata tctttatcat tcttgccgtt tctggtgttt
     6481 gcttctgatg cgtcgattag tctggttggt gttcaaggct atgatttggt ttcttatcat
     6541 cagaaaaatg gtcctgtccg tggtagtggt aaccacactg cttatcataa tggagtagct
     6601 tacctattcg ctactgatga aaacaagaaa gtgtttcagg ctaatcctga aaaatatttg
     6661 ccagcatatg gtggttattg tgcttttgca gtttcagtag gtcggaaagt agtgagtgat
     6721 ccattagcat ggaaaattgt tgatggcatt ttatatctca atcttaataa acaggtacaa
     6781 gaattttggt caaaagatat tcccgggaac ataaaaaaag gcaatattca atgggaaaaa
     6841 attaaggata ttgaccctca aaaaatttaa tgcaattgca atatccctaa cctgtaccat
     6901 tctgttttat cgattattat acaggtttaa tgcaaattat tttattttta taatggccac
     6961 acccataaaa tcgttcgaat agaagccatt ataacaataa gttccaatgg taatcccaaa
     7021 cgaaaataat cagaaaatcg gtatcttcct ggccccataa ccatggtatt gttctgatgg
     7081 gcaattggag ttaggaatga acacgaagca ccaatagcaa ctgccattaa gaaagaatcc
     7141 acattcagat gggctgactg agcaattttg attgcaatgg gagccataat taatgccgtt
     7201 gctgcattgt ttaataaatc ggacaaagtc atagtgataa ccagaatcgc aagcaaggca
     7261 aagatagggg ggaatattgg ccagcaattt tcataaatcc atttgcaatc aaatc
//
LOCUS       DACTJE010000161         7221 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.162, whole genome shotgun sequence.
ACCESSION   DACTJE010000161 DACTJE010000000
VERSION     DACTJE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.162"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(104..2755)
                     /gene="traA"
                     /locus_tag="I9760_002517"
     CDS             complement(104..2755)
                     /gene="traA"
                     /locus_tag="I9760_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ti-type conjugative transfer relaxase TraA"
                     /protein_id="HAT5044270.1"
                     /translation="MAIAFAQVSIHSRSKGHSAVAAAAYRSGARLYDDRIGRAYDFSK
                     RDDVAFSEILLPDGTTDSFLERDYLWNEVERAENRSNSQLCKDFVLALPRELNLEQQI
                     ELAKRFARTHFVEKGLPADIAIHDHGDGNPHAHILIPTRRLENNRFSKYKARDLNPVF
                     AKGFLVEQDYWGEQWREMQNEFFIENNLDLQVDANHLIAERHRGKHHNANAHYLLEEN
                     QLIQQARMELTRDNIDLVIDHLSTQYSVFTRRDVERLLFKTFQASDSPQEYLQFVERV
                     LGHQDLVALGDNNKGEPSFTTRTHLMQEAQLLHDIEAMMVSKNHVVHQPIDSLAQQYR
                     LSEEQYEAFRYITQSPDISVVIGRPGTGKSYLLKPLNEYYIQAGMEVIGAALSGKVAK
                     ALQAETGITSSTIKSLSYRLANDMMQLSDKHVLIIDEAGMVDFSSMGYLIKEAHKAGS
                     KVVLIGDPDQLKPIQKGEIFRGIAARTGYIELENIKRQQDLGDRQASLNLAKGDIDKA
                     IQHYQDKGAITITDTKLQAIEQVVADWKQDLESTNMADSIMLSYTRKAVNHLNDLARE
                     ALIVENKIGEENIVYQGLERSLKISTGERLLLRENNKVLGVRNGDTATVKEINVQQMK
                     IQLDSGELLIIPKEYKALDYAYALTVHKSQGMTAKKVRVLIDSKYWDRNLSFVAMTRH
                     KEQLNIYADKENHPTEQALKQTLSRSSTKDNVIDWPLDFAIRAGFDSDSLISRALNHI
                     VGVGHKIKQAYNFIVNYEASLLKARHQERFQDIGQAIADAKQEALKVDTKEFESNDCK
                     TSEQSFGILKKDFPQLVELETLIKRRQRMTGYFAEKTDKQIMALTQQLLTNKTFANQV
                     KYQVPEFYQKLQSIDNKQKEKALLQNH"
     gene            3000..3206
                     /locus_tag="I9760_002518"
     CDS             3000..3206
                     /locus_tag="I9760_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044271.1"
                     /translation="MSKKTVSQMEALLMDKIEQAKVRLDKLQQKHKIEIGMLAYKHGL
                     QNIDTKQLDNAFAQMAKELIHEHA"
     gene            3193..3456
                     /locus_tag="I9760_002519"
     CDS             3193..3456
                     /locus_tag="I9760_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HAT5044272.1"
                     /translation="MNTLEQIKKEKQAISKSKQSLALAKLKKRRADTRCKIELGGLVI
                     KAGMVHFNKSIILGCLGFTTKLIEKQPEYKKFFEEIGNELFLK"
     gene            3473..4264
                     /locus_tag="I9760_002520"
                     /pseudo
     CDS             3473..4264
                     /locus_tag="I9760_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4623..5333
                     /locus_tag="I9760_002521"
     CDS             4623..5333
                     /locus_tag="I9760_002521"
                     /inference="COORDINATES: protein motif:HMM:NF021095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco47II family restriction endonuclease"
                     /protein_id="HAT5044273.1"
                     /translation="MPYVSFIDDNTFENIVSDTINVASNAMSNSKKKFEKNVIDPFLT
                     IFEISGFRIDSTEWLEHEKWRQAQKSLSNSIGIFHQKLLGSINGWECLPTGHIVDIVN
                     HEKRIIAEIKNKYNTIKGSAKAKLYHDLDDLVMQKKQEYKEYTAYYVEIIPKKPERYN
                     QPFTPSDSRKGAKCAANEKIRVIDGYSFYSLASGIQNALEAIYQALPLVIQKILPDIN
                     LAELHSVMKYFNQAFGSK"
     gene            complement(5308..6558)
                     /gene="dcm"
                     /locus_tag="I9760_002522"
     CDS             complement(5308..6558)
                     /gene="dcm"
                     /locus_tag="I9760_002522"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAT5044274.1"
                     /translation="MSYDLIEDAPFVSISEAATALDASIDTIRRWEKKGLIKAHRSDT
                     GHRLFQITELQRLKAKLFGAGDDHCYKILTAPSRTQFKVVELFAGAGGLALGFHNAGL
                     DCSMLVEIDKNPVETLRHNCPSWNVIHDDIANVDFQGITADVVAGGFPCQAFSYAGKK
                     LGFEDTRGTLFFEYARAIKEINPKVIVGENVRGLEKHDSGRTLNTMLQILDQLGYDVE
                     YKILRAQYLDVPQKRERLVMLGIRKDLKGTIAFPKEQDYTISLREALKDVPDAPGQKY
                     TEKKSKVMSLVPEGGYWRDLPDDKQKEYMGASYFLGGGKTGMARRLAWDEPSLTLTCN
                     PAQKQTERCHPKETRPLNVREYARIQTFPDSWMFSGTVSSQYKQIGNAVPVNLGYHIG
                     RCVIAMLTQRFDRKDLIICSRKPD"
     gene            6714..7142
                     /gene="vsr"
                     /locus_tag="I9760_002523"
     CDS             6714..7142
                     /gene="vsr"
                     /locus_tag="I9760_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch endonuclease Vsr"
                     /protein_id="HAT5044275.1"
                     /translation="MEGNFLDMISKKQRSHIMSSIKGKNTKPEIIVRSFLHRQGLRFR
                     LHSKNLPGQPDLVFRKYNAVVFVNGCFWHGHNEPLCKNSHIPKTNFEYWWNKIERNKS
                     RDKQNIEILKQLGWRVFMIWECELKNHCFLLKLLASIKSE"
BASE COUNT         2281 a         1407 c         1247 g         2286 t
ORIGIN      
        1 ataatctaag tcttgatcgt ataaacaaat actcgtaaaa gtaatgcacc ctaaaattac
       61 caacttgcgg tataaggagg catttaatag ttgattgcta gcatcagtga ttctgaagta
      121 aggctttttc cttctgttta ttatcgatac tttgtaactt ttgataaaac tcaggaactt
      181 ggtatttgac ttgattggcg aaggtcttat tcgttaataa ctgctgtgtc aaagccataa
      241 tttgtttatc agttttttca gcaaagtaac cagtcattcg ctgtcgcctc ttgataagtg
      301 tttcaagctc aacaagttga ggaaaatctt tcttcaaaat tccaaaggat tgctcagatg
      361 ttttgcaatc attgctctca aactctttag tatcaacttt aagtgcttcc tgtttagcat
      421 cagctattgc ttgtccgata tcctgaaacc gttcctgatg ccttgctttc aaaagagagg
      481 cttcataatt aacaataaag ttataagcct gctttatctt atgccccact ccgacaatat
      541 gattcaacgc tctactaatc aatgaatcag aatcaaaccc tgctcggata gcgaaatcca
      601 atggccaatc aattacgtta tctttagtac tgctgcggga taaagtttgt tttaaagcct
      661 gctctgttgg atgattttct ttgtccgcat agatattaag ttgttctttg tgacgtgtca
      721 tagcaacaaa acttagattt ctatcccagt atttgctatc aattagaacg cgcacttttt
      781 ttgcagtcat gccttgggat ttatgaacgg tcagtgcata tgcataatct aatgccttat
      841 attcttttgg aataatcagt aactcaccac tatccaattg aatcttcatt tgctgtacat
      901 ttatttcttt aaccgtagct gtatctccat tacgaacccc caggactttg ttgttttccc
      961 taagcaataa tcgctcacct gttgatatct tgaggctccg ctctaaccct tgatagacga
     1021 tgttctcttc accaatttta ttctcaacta ttaacgcttc acgagccaag tcattgaggt
     1081 gattaactgc ttttctggta taagacaaca tgatagagtc cgccatattc gttgactcaa
     1141 gatcttgttt ccagtccgca actacttgct ctatggcttg cagcttggta tcagtaattg
     1201 ttatcgctcc cttatcttga taatgctgaa tggctttgtc tatatcccct ttggctaaat
     1261 tgaggctcgc ttgcctatcc cccaaatctt gttgtcgttt gatgttttca agctcaatat
     1321 aacccgtgcg agcagcaata cctctgaata tttcaccttt ttgaataggt tttaattgat
     1381 ccggatcacc aatcagcacc actttactac ctgccttgtg tgcttcttta atcaggtacc
     1441 ccatactgga aaaatcaacc atccctgctt catcaataat caggacatgt ttatcactta
     1501 actgcatcat atcattggct aatcgatagg atagcgattt aatcgtagaa gaagtaatgc
     1561 ctgtttccgc ttgcaaggct ttagcaacct taccagataa tgcagcacca ataacctcca
     1621 tccctgcttg gatataatac tcatttaatg gctttagcaa atagctttta cctgttcccg
     1681 gccgtccaat cactaccgaa atatcagggc tttgagtaat ataacgaaag gcttcgtatt
     1741 gttcttcact taatcgatat tgttgggcaa gactatctat gggttgatgg actacatggt
     1801 ttttagaaac catcatcgct tcaatatcat gtaacaactg ggcttcctgc attaaatgtg
     1861 tgcgtgtagt aaagcttggc tcccctttat tattatctcc caatgctact agatcctgat
     1921 gacccagtac tctttcaaca aactgtaaat attcttgtgg gctatccgat gcttgaaacg
     1981 ttttaaataa taaacgttca acatccctgc gagtaaaaac actgtattgc gtagataaat
     2041 ggtcaatcac caaatcgata ttatctcgag tgagctccat tcgcgcctgt tgaatcaatt
     2101 ggttttcttc aagcaggtag tgtgcattag cattgtgatg cttaccgcga tgacgttctg
     2161 caatcaggtg gtttgcatcc acctgtaaat cgagattgtt ctcaataaaa aattcgttct
     2221 gcatctcccg ccattgttca ccccagtaat cttgctccac caaaaacccc ttggcaaaaa
     2281 ctggatttaa atcgcgagct ttgtatttgg aaaatcggtt attctccaat cttcgtgttg
     2341 gaatcagaat gtgcgcatgt ggattgccat ccccatgatc atgaatagcg atatcagctg
     2401 gtaaaccttt ctccacaaaa tgcgttcgag caaaccgttt tgccaattca atctgctgtt
     2461 ctaaattcaa ttcacgcggc aatgctaaaa caaagtcctt acataattga gagttcgatc
     2521 gattttcagc ccgctctact tcattccata aataatcacg ctccagaaat gagtcagtag
     2581 taccatcagg caaaagaatt tcagaaaaag caacatcatc tcgctttgaa aaatcgtaag
     2641 cgcgaccaat gcgatcatca taaagccgag cacccgaacg ataagcagcc gcagctacag
     2701 cactatgacc ttttgaacga gagtgaatag aaacttgtgc aaaagcaatc gccacggaat
     2761 ccccaaagag aatgaatcca tcggttgctt tttataatga aaaaaccttt gtccattaaa
     2821 gatttttaag tgtttgaatc atgatactag caaacaaacg aaagtttgta tattgcgccc
     2881 tttgcttttc gcttcgggat tttgaagtta gctacgctca cagattggca cagcctattg
     2941 attttcgaga tcccgctagt tatgctgaac aagttagtcc attattgcgg agaaataaaa
     3001 tgtcaaagaa gacagtatcc caaatggaag cactacttat ggataaaatc gaacaagcca
     3061 aagtaaggct tgataaactt caacaaaagc acaagatcga aataggcatg ctcgcctata
     3121 aacacggctt gcaaaatatc gacaccaagc aactggataa tgcctttgcc caaatggcta
     3181 aggagttaat ccatgaacac gcttgaacaa atcaaaaaag aaaaacaggc catttcaaaa
     3241 agtaaacagt cactggcttt agcaaaactc aaaaaacgcc gggcggatac tcggtgtaag
     3301 attgagttgg gtggattggt tattaaagcg gggatggtac attttaataa atcgattata
     3361 cttggatgct tgggcttcac taccaaactt atagaaaagc aacccgaata taaaaaattt
     3421 tttgaagaaa ttggtaatga attattttta aaatgaatca taagcttttc ccatgtcctc
     3481 accacctgat gttataaaaa acgaaaataa cgattttgaa attgtctctc cgaggccata
     3541 tggcctcatt gaggatttgt gtaaggtaaa atataaaaac agggcttgca tgtatcctgg
     3601 atgtaatcag ccaacgataa attctcatac tttatcgaaa gcatctatcc ggaaatatgc
     3661 aaataatcaa attattaatt taaagacgat taaccatgac ctgtttgaaa atataaagcc
     3721 tacagtaaat cgcaagtatt ataaaccctg tgatctaaat aaaatatcaa ccttcaatgg
     3781 cttctgtagt gaacatgata atagcatctt ctatgaatta gataattacg atggcaatat
     3841 aaccccgcag atcgctcttc ttgggcatta tcgaataatt tgttacgggc taaacaccat
     3901 ccgactccaa ttaaaacaaa atgaattttt gagaggtgct aaatttgtag gagctgcaac
     3961 aaaaaaaata aatgaaattt acagaaaaat taaaaatggc ttttatgagc gcaggttaaa
     4021 aatggcagaa acagattatg taaggagaaa aatgatctgt gaagatatat tggctattgg
     4081 tacaacgcat gatattaagt ttgttaccct gcaaggcaca atgagtgatc cattattttt
     4141 tggaagagct ggaatatttt tgcatcaatt tggtaaggaa aggttacctt taaggtttat
     4201 gctccaaatg ccatacatat cttatttttc tatttgtgat ggaaaaacat gtaaattaat
     4261 ttttgtttat cttgctcaag attcaaacga atacgaacgt gatttggaaa attttcttaa
     4321 tcatgtagat ctaaaaaaac gcttggagat tttaatctac ccacatagcg attgttgtat
     4381 cttaagaaat gatattccac aaaccagtga taacactatt aagcaactga taaattttta
     4441 ccgataatag tcaatgaatt gtcctggttc tcaacttgta gccaatacca agccatttac
     4501 tacatctatc actatgtaca atctgaactg aaaattaatt cgtgctaaaa attcaaccaa
     4561 attttgattc aattcggtaa ttgatatatg atgctcattt tataatcctc caagccagaa
     4621 aaatgcctta cgttagcttt attgatgata atacttttga aaatatcgtt tcagatacta
     4681 ttaatgttgc aagtaatgcc atgtctaatt caaaaaaaaa atttgaaaag aatgtaattg
     4741 atcctttctt aactattttt gaaatttcgg gatttcgtat tgattcaaca gaatggttgg
     4801 aacatgaaaa atggcgtcaa gcacaaaagt ctttatccaa tagtatcggg atttttcatc
     4861 aaaaactatt aggtagtata aatggctggg agtgtttacc aacagggcac atcgtagaca
     4921 tagttaatca tgaaaagcga ataattgcag aaattaaaaa caagtacaat acaattaaag
     4981 gatctgccaa agcaaaactg tatcatgatc ttgacgatct cgtgatgcaa aaaaaacaag
     5041 aatacaaaga atacactgcc tattatgtag aaattattcc taaaaaacca gaaaggtata
     5101 atcaaccctt cactccatca gattctcgta aaggagctaa atgtgcagca aatgaaaaaa
     5161 tcagagtcat tgatggttat agcttttata gcctagcatc aggaattcag aatgcacttg
     5221 aagccattta tcaagcatta cctttagtga ttcaaaaaat tctgcctgat ataaatcttg
     5281 ctgaattgca tagtgttatg aaatatttta atcaggcttt cgggagcaaa taattagatc
     5341 tttcctatca aacctctggg taagcatagc aataacacac ctgccaatat ggtagcccaa
     5401 gtttacaggc acagcattac cgatttgttt atactgcgat gatactgtgc cagagaacat
     5461 ccatgaatca ggaaaggttt ggatccttgc atattcccta acatttaatg gcctagtttc
     5521 tttaggatgg caacgttcag tctgtttttg ggcagggttg cacgttaggg ttaaggatgg
     5581 ttcatcccat gccaaacgcc tggccattcc tgtttttcct ccacccaaaa aataactggc
     5641 tcccatgtat tccttttgtt tgtcatctgg taaatctctc caatagccac cctctggaac
     5701 tagcgacatt accttagatt ttttctcagt atatttttgg ccaggagcat caggtacatc
     5761 ttttaaagcc tctcttaggc taattgtata atcctgttct ttgggaaatg cgatagtgcc
     5821 tttaagatct ttcctgatgc ccagcataac aaggcgttcg cgtttttgag gcacatctaa
     5881 gtactgggca cggaggattt tgtattcaac gtcataacct aattgatcta aaatttgcag
     5941 catagtattt aatgtccggc cactgtcatg tttttccaat ccacgaacat tttcaccgac
     6001 aattacttta gggtttattt ctttaatcgc tctagcgtat tcaaaaaata aggttcctct
     6061 ggtgtcttca aaaccaagtt ttttcccagc atagctgaat gcctggcagg gaaatcctcc
     6121 tgcaactaca tcagcagtaa ttccttggaa gtcaacattt gcaatatcat catgaataac
     6181 attccatgat gggcaattat gccttaacgt ttcgactggg ttcttatcaa tttcaaccaa
     6241 cattgaacaa tctaaaccag cattatggaa accaagcgcc aaaccaccag caccagcaaa
     6301 tagttcaact accttgaatt gtgtcctgct aggggctgtt aatatcttat aacaatgatc
     6361 atcaccagca ccaaacaatt tggcttttaa cctttggagt tcagttattt gaaacagccg
     6421 atgccctgta tcactcctgt gtgcctttat taatcctttc ttttcccaac gccttatggt
     6481 gtcgatagaa gcatccagtg cagttgctgc ttcactgatt gaaacaaaag gagcatcttc
     6541 aataagatcg tagctcattg tttttcctta tttttattct tatatcaata taatagatat
     6601 aaccattgcc tatgtaaagg ttacagttaa tttggatcaa atataatttt tttgaggagg
     6661 tatcaattta aatcctcaca ttaataagct ttttatatct tatacttttg gatatggagg
     6721 gcaatttttt ggatatgatt agtaaaaaac aacgtagtca cataatgagc tcaattaaag
     6781 ggaaaaatac taaacctgaa ataattgtgc gttcttttct acaccgccag ggtcttcgat
     6841 ttagattgca tagcaagaac cttcctggtc agcctgatct tgtgtttaga aaatacaacg
     6901 cggttgtttt tgttaatggg tgtttttggc atggtcataa cgaaccatta tgtaaaaatt
     6961 ctcatatccc taaaacaaat tttgaatatt ggtggaataa gattgaacga aataagagtc
     7021 gagataaaca aaatattgag atattaaaac aactcggttg gcgtgttttt atgatttggg
     7081 aatgcgaatt aaaaaatcat tgctttttgt tgaaactatt agcttctata aaaagcgagt
     7141 aattctatgt tgtgaaaata ttttttcctc aggataaaaa actatttcct aaaattaaaa
     7201 ataaggatgg aaatttattt g
//
LOCUS       DACTJE010000162         7192 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.163, whole genome shotgun sequence.
ACCESSION   DACTJE010000162 DACTJE010000000
VERSION     DACTJE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.163"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(140..229)
                     /locus_tag="I9760_002524"
                     /pseudo
     CDS             complement(140..229)
                     /locus_tag="I9760_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652674.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            343..957
                     /locus_tag="I9760_002525"
     CDS             343..957
                     /locus_tag="I9760_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044276.1"
                     /translation="MLLKYLFTVTILITSSIVFAQLPKEVIDSCHDGVADNRDSVIYM
                     YLPNDLWRLAPRSITPVKNDVEHIELGGSYDYEEINGSEERYQFDFFMHHFSIEDNQY
                     FVRSDSRTKLSDAINHSNHSIAQPGNINNLMIVGKITFNKKEYLCISGPLYQNGTFAA
                     VAEYYIVENAFDYDKKIQLHYYFFDREFVEMALIRDNWDGHWED"
     gene            complement(1113..1319)
                     /locus_tag="I9760_002526"
     CDS             complement(1113..1319)
                     /locus_tag="I9760_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044277.1"
                     /translation="MNINELIEHWKYISSIVRAPQSTEDYNQLANVLDQLLDIVKDDE
                     SHELMGLVDIISHMISIYDETHDY"
     gene            1656..2924
                     /locus_tag="I9760_002527"
     CDS             1656..2924
                     /locus_tag="I9760_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2372 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044278.1"
                     /translation="MKVKYSLFVIDKIKKYNYQRASNYRSTIMSTTIDEATKRLDNMV
                     KLLKEFKKEVKAQKFKSERNFENSSTIFAEFNSELNELKKLKHHNLARVIVEYLGQVI
                     EKNDLNMKLIITNKDTTQNIVELEMTSNLKKKEFYKKGIVIATDTSAINHELMKYVSE
                     VTVNCHWSRKNRTSYNSTRELVDTVANELKHEKQYPRLKRFTILGCDTADIIPPEVET
                     KSSSAMLYVYQCEKNNAATPDRKKIQSFPCFVLTISQGQYQLHYYIDQDKEGTCGEMV
                     KITKQKKLQNNYKVLAVCNKNDIPLIPDSILKNIYDKTNSAKINGVNNPYLHRGATVG
                     NVQSREEWRNRKKNIPALSCGLFKFDNKKIHNSNLAESAALIINNSRSNVKVKGYLIP
                     ITPDENHGFMKPMTIHEDTPCKAVIIDHGQ"
     gene            complement(3140..3665)
                     /locus_tag="I9760_002528"
                     /pseudo
     CDS             complement(3140..3665)
                     /locus_tag="I9760_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947272.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3665..4330)
                     /locus_tag="I9760_002529"
     CDS             complement(3665..4330)
                     /locus_tag="I9760_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044279.1"
                     /translation="MSDFVYVLMERRSRNKTSEFVFQAESKTGYIYEPKKEVNRAVES
                     SGVTYTLRFHMMDVFFVLVFLFSLLSSNAYSEKVHSISLLTYPVEAIYVINNPRTGEM
                     VIGSDYHGACNLCGIDSPGVELAYKAPRNVLNDGTDYSNGACFGDGVDLKVNGENAYR
                     YKARFKLANKNHNVLSVIEPPPLQRRGKNNKYFEYTYISTSGINKTIFWKYTKEALEA
                     GET"
     gene            complement(4684..4995)
                     /locus_tag="I9760_002530"
     CDS             complement(4684..4995)
                     /locus_tag="I9760_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAT5044280.1"
                     /translation="MQIIKSSLFNGDFPWQALDIAQISDASIRLHWADQPYIWHKNDG
                     DEVFVVLSGKVIMHYREGGVENQKELNSGDICYLKEGCEHVAYPKGAARILVIEKIGS
                     I"
     gene            5443..5916
                     /locus_tag="I9760_002531"
     CDS             5443..5916
                     /locus_tag="I9760_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044281.1"
                     /translation="MRSRTEKLSSKRSIINHEHLITDEVCMIGIYREGGDLSIYAESP
                     EGIEHFSINRGNSLDMVILPHTLVGTWRHHQLLRTVEQAAQIRALRIRSHDYIEQSVT
                     RKELEQFKEVFMLACKEEISNQPEIEEYLQTALGKLHGEPEMTYKPKFTEQCSIL"
     gene            6108..7025
                     /locus_tag="I9760_002532"
     CDS             6108..7025
                     /locus_tag="I9760_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT5044282.1"
                     /translation="MDKLNLNLLRALYALLTCRHLTLASKKMGISQSSMSIYLKQLRE
                     HFHDDLLVRGQGNIMQLTPLANSLIGKARQALSLIETLFNTIETFNPLISERSFSIGM
                     TDLMSILLTPSLIKRLEQEAPGVKLNIKHPSYLNTVEVFESGDIDLMIGMFERVPEGL
                     KQQKLFQDEAVIVGGNNHPGIQKGKICIEELFKYPLIQFSLRETPFYNYFDRYLRQLG
                     HDKRVSISLGQGIMPLLVLTDSHYLTLSIRKVAEKLSHLIPLSIASVPFEVDSFNCYQ
                     YWHQKDDADPGHRWLREMILSIAKKSYLK"
BASE COUNT         2472 a         1299 c         1171 g         2250 t
ORIGIN      
        1 ccaggccaag tatacagaaa tcctatattt acataggatt tctgtatact taacaaaata
       61 atcgcttttt gccagttcat gctaacattt tgatgttttc ttacatgatg catgtatctg
      121 aaacaatata gctttaaaat taatcaataa aagtagctgg aataacatga aatctcgctg
      181 ctaatttctt gatatgagaa atgttcagct atcacttgcc gtttaatatc ccaatttatt
      241 aagcttaaaa ttctgaggaa atcccttgct acatttaatc agaataagta taagcttttt
      301 caaaactagc acatcctcat catattttgg tctaagaaaa aaatgcttct aaaatactta
      361 tttacagtta cgattttaat cactagttca atagtttttg cccaactacc caaagaagtc
      421 atagattcat gtcatgatgg agtagctgac aatagagata gtgttatcta catgtatctt
      481 cctaatgatc tatggcgctt ggcacctcgt tcaataactc cggttaaaaa tgatgttgag
      541 catatagagc taggtggaag ttatgattat gaagaaatta atggcagtga agaacggtat
      601 caatttgatt tctttatgca ccattttagt atagaagata accaatattt tgtaagaagt
      661 gacagtagga caaaattatc cgatgcgatt aatcattcga accactctat tgcccagcca
      721 ggaaatataa ataatctcat gattgtagga aaaattacat tcaacaaaaa agagtaccta
      781 tgtatatctg gccccttata tcaaaatggc acattcgctg cagttgcaga gtactatatt
      841 gttgaaaatg cttttgatta tgataaaaaa atacaattac attattattt ttttgacaga
      901 gaattcgttg agatggcgtt aatcagagac aactgggatg ggcactggga ggattaaaat
      961 acgaccaatg gattactcag ctaattaact aatttacagt aaattcagct attaaaataa
     1021 tttgatcgca atacgaattt agtgaatatg gaagaattag tatgattaaa cctaccacca
     1081 taaacagaga agattttaat ttattaaatt tatcaatagt catgagtttc atcgtatata
     1141 gaaatcatgt gactgataat atcaactaat cccattaatt catgagactc atcatccttg
     1201 actatatcca acagctgatc aaggacatta gccaactgat tataatcctc cgtactttga
     1261 ggcgcccgta ctattgatga gatatatttc cagtgttcta ttagttcatt gatattcata
     1321 attcagccat ctccatcata tgctcaagta taaaattaac aacctgctgc attggcttac
     1381 gcaaccgatc aacatcttta tatatcctgc tgtaacatca ttattcattt gaaacatctt
     1441 ggtagctatc atgaagtcgg tgataactcc ataccggaag gctgattatc tctacgggat
     1501 aacgatatct tttcggcctt tgctccgaca tctttccaca ggtaaataga gcatagtgta
     1561 cacaaaaagt taagttgccg tcccaggcct aataggagtt tgggctgctt tattgttgga
     1621 attgaaaata tactttaagg aacatttcag cccaattgaa ggttaaatat agcctgtttg
     1681 taatagataa gatcaaaaag tataattatc agagagcatc aaactataga tcaactatta
     1741 tgagtacaac tattgatgaa gcaactaagc gcttagataa catggttaaa ttattaaaag
     1801 agtttaagaa ggaagtcaaa gctcaaaaat tcaagtcaga gagaaatttc gaaaattcca
     1861 gtaccatttt tgcagaattc aatagcgaac tgaatgaact aaaaaaatta aaacatcata
     1921 atcttgcaag agtaattgtg gaatatttag gtcaggtcat cgaaaaaaat gatctcaata
     1981 tgaaactgat aattacaaat aaggatacga cacaaaatat agttgaactt gaaatgacta
     2041 gtaatctcaa gaaaaaagag ttttataaaa aggggatcgt gatagccaca gatacatcag
     2101 ccatcaatca tgaattaatg aaatatgtta gtgaagtcac ggttaattgt cactggtcca
     2161 ggaaaaatag aacatcctac aacagcacga gggagctagt tgatactgtt gccaacgaac
     2221 tgaagcatga gaagcagtat ccgcggctca agcggtttac cattttaggc tgcgacacag
     2281 cggatataat ccctcccgag gttgaaacca aatcatcttc tgcaatgtta tatgtttacc
     2341 aatgtgaaaa aaataacgct gctaccccag acaggaaaaa aatacaaagt ttcccctgtt
     2401 ttgtactcac aatttcacaa ggacaatatc agctccatta ctatattgac caagacaaag
     2461 aaggaacctg tggagaaatg gttaagatta ctaagcaaaa aaaactccaa aataactata
     2521 aggtgttagc ggtttgcaat aaaaatgaca tccccctcat acccgatagt atcttaaaga
     2581 acatttatga taaaacaaat tctgcgaaaa taaatggtgt aaataacccc tacttacata
     2641 gaggcgctac cgtaggcaat gtacaatcaa gagaagagtg gagaaatcgg aaaaaaaata
     2701 ttcccgcact ctcgtgtggt ttatttaaat ttgataataa aaaaattcac aactcaaatt
     2761 tagctgaaag tgcggcttta attatcaata atagccggag taacgttaaa gttaaaggct
     2821 atttaatacc aattactccg gatgaaaacc acggatttat gaaaccaatg acaatccacg
     2881 aggacacgcc ttgtaaagca gttatcattg accatgggca gtaaatcgtt cacgatctga
     2941 ccctagtccc gttcgcgaat tgatgacgtt atttgatggt aaatcagaac aggcggagtt
     3001 ttatatttaa gatttgagag tttgcaggat gaacaaagca aagcgtgtcc accgggacaa
     3061 aattgggcta tgatcggtag gcacgtcact gggttccttt gtccatccta tatatctggt
     3121 aaattggctt ttaatcaatt caaaaacgct tatagacatt catgtcgaaa aaataatagc
     3181 taatattata attttttttg ttcacatcaa aagcatcttc aactagataa tattaccagg
     3241 aattagcagc agatcctgta tctgagagag atgtttgtat acatagataa gatttatcgc
     3301 ttttatttat tctccaccac tcagaaaaac gaagaattgg gatatctggt ttaacacttg
     3361 gattcatcga atagttttta gctgttgctg tacttatttt ttatttgcaa ttattaaata
     3421 aggttggtca tcacacacta ccgctccata gagctgacca ttaatttcag aatcgaaatg
     3481 atttctacaa cctggttcat tatctttagt gctgccaaac ccatccagta aggtcatggt
     3541 tatagaatca ttggctggct tactgttttt acatgattgt aaaaattcta ctggggcata
     3601 gccaatacaa tagagcttat gaatatacaa attaaaatta caacaaattt ttcccttaaa
     3661 tacattacgt ttctcctgct tcaagcgcct ctttagtgta cttccaaaat atagttttat
     3721 tgatgccaga tgttgaaata taagtatatt caaaatactt attatttttt cctcttcttt
     3781 gtaatggtgg tggttcaatt acagaaagaa cattatgatt tttattagca agcttaaatc
     3841 ttgctttata tctataagca ttttctccat ttaccttcaa atcaacccca tccccaaaac
     3901 atgccccatt tgaataatca gttccatcgt ttagaacatt cctgggagct ttatacgcta
     3961 attctacccc tggggaatcg attccacata agttgcaagc cccatgataa tctgagccaa
     4021 tgaccatttc accggttctt ggattattga tgacgtatat tgcttcaaca ggataagtta
     4081 atagagaaat actatgcact ttttcgctat atgcattact agataacaag ctgaacaaaa
     4141 ataccagtac gaaaaaaaca tccatcatat gaaaccgcaa ggtatatgtt acgccagacg
     4201 actccaccgc cctattcacc tcttttttag gttcatagat atagccggtt ttactttcag
     4261 cctggaatac aaattcactg gttttatttc ggcttctgcg ttccattaac acatagacaa
     4321 aatcagacat aggcaagatg tatttcttga tttttgggtc tattcgcgcc taatttgaaa
     4381 tcattttgtt taaatcagat tgcaatataa aatctttaaa caattgatgt atataaagaa
     4441 cgcttttttc tttgtgacat accatataat actcattatc cacattaagc caatcaggta
     4501 ataccttaac caacgttttg tgtttcaaag catctgaaca taatcggcag tgcagaaaac
     4561 tctttgaaat attcccctgg cttgcctttt aattttttaa atttaggagt ttttaacttg
     4621 cctgcagagt cactcatcaa taaatatcct tttttggaga ttacctgaaa acataaatca
     4681 attttaaata ctaccaattt tctctatgac aagtattctt gcagcccctt ttggataagc
     4741 tacatgctca catccttctt tcaaataaca tatgtctcca ctatttaatt ctttttgatt
     4801 ttctacacca ccctcacggt agtgcataat cacttttcca gacagcacaa caaaaacctc
     4861 atccccatca ttcttatgcc aaatatatgg ttgatctgcc caatgaagtc taatgctcgc
     4921 atcagatatt tgagcaatat ctaatgcttg ccatggaaaa tcgccgttaa ataatgatga
     4981 tttaattatt tgcatattac cttacgctta ttaaatttta gtggttttag attctggcta
     5041 gcagccagaa cgaataatcc tttttgctcc aatttcctaa ctcaattata atcactttca
     5101 acttgttctt tcgattcatg gcgtagagtt aatagaccag gcatgggaaa aagatcgact
     5161 gttttcttaa gatacttacc aggtcgaacg ttaaggttaa gcgttacaaa actaattaat
     5221 tttttaatat actctagatt ttgatgttca tctaataagt tgatcatcta catcaccatt
     5281 catcctccag aattttaata ctcattatta aaatttcatt aagaatttta agaatacgct
     5341 aattattcat cgataaaatg acacttatca attcaataag atcaaaaatt attcatattg
     5401 aagcgatgaa tttattaaat aattgtaaaa taggagttat ccatgcgtag cagaacagaa
     5461 aaattaagca gtaaacgttc gatcatcaat catgagcatt taatcactga tgaagtctgt
     5521 atgataggaa tttatagaga aggtggtgac ttatcaattt atgctgaatc acccgaagga
     5581 attgaacatt tctctataaa tcgaggaaac agtcttgata tggtgatatt accacatact
     5641 ttagttggaa cttggaggca tcatcaatta cttcgtacgg tggagcaagc agcacagatt
     5701 cgagcattaa gaataagatc tcatgattac atcgaacaat cagtcacccg aaaggaactt
     5761 gaacaattta aagaggtatt tatgctcgca tgcaaagagg aaataagcaa tcaacctgaa
     5821 attgaagagt atctccaaac cgctttgggt aagttacatg gggaaccaga gatgacttac
     5881 aaaccaaagt tcacagagca atgcagcatt ctctaaaatt tttattagaa taatcagaga
     5941 aaaagctcga gattcaaaat cttcgagctt ttttaatatc cttcatcagt tcgattagta
     6001 ccaggcttat ttgcaaactg tatcgctctc aactaacctc aaacaaaaag ccgttaaaaa
     6061 tagagtcatt taaaacctat tagggtccta tatacggatg atgttttatg gataaactta
     6121 atctcaattt acttcgcgct ctgtatgcac tattaacctg ccgtcatcta acacttgcta
     6181 gcaaaaaaat gggtataagc caatcctcaa tgagtattta tttgaagcaa ctgcgtgagc
     6241 atttccatga tgatttgctt gtacgtggcc aaggcaatat tatgcaatta actcctttag
     6301 ctaactcgtt gatcggtaaa gcccgacaag ccctctcact gattgaaaca ctatttaata
     6361 ctatagagac ctttaacccc ttgatttcag aaagaagttt ctcaattggc atgactgatt
     6421 taatgtctat tttgttgact ccaagtttga ttaaacgatt ggaacaagag gctccaggag
     6481 tcaagctaaa tataaaacat cctagttatt taaatacggt ggaagtgttt gagtccggtg
     6541 atattgattt aatgattggt atgtttgaga gagtcccaga aggcttaaag caacaaaaat
     6601 tgtttcaaga tgaagccgtc attgttgggg gaaacaacca cccaggaatc caaaaaggga
     6661 aaatttgcat tgaagaacta tttaagtacc cattaattca attttcatta agagaaactc
     6721 ctttttacaa ttatttcgat cgctatttac gtcaactggg tcatgacaaa cgagtttcta
     6781 ttagcttagg acaggggatc atgcctttat tggttcttac tgactctcat tatcttacat
     6841 taagtattcg aaaagtagct gaaaaacttt cccatttgat accattaagt attgcctctg
     6901 taccctttga ggttgacagt tttaattgct atcaatattg gcaccaaaaa gatgatgctg
     6961 atcctggtca tcgttggtta cgggaaatga ttctcagtat agctaagaaa tcttatctaa
     7021 aataatcagt taaaacccac tctacgaact agctatagat tgatataaaa cacatatatt
     7081 ttagataatc acagaaaagt ctcttttaat agaccatgaa tcaggactgg tattactagc
     7141 aaatcgcaaa taataaaaac taaatggcga taaaatgcac catgaaacca ac
//
LOCUS       DACTJE010000163         7094 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.164, whole genome shotgun sequence.
ACCESSION   DACTJE010000163 DACTJE010000000
VERSION     DACTJE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.164"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            510..1376
                     /locus_tag="I9760_002533"
     CDS             510..1376
                     /locus_tag="I9760_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0189 family type II secretion system
                     effector"
                     /protein_id="HAT5044283.1"
                     /translation="MKPGYLLGALLLSPILAFANSDNNTDGLIFSPLPQNKNTVVRHY
                     SNEQEMPNLSQMAQRTIDFPTQIVRVSGNLTGLELSCDDVENEIDQVFSKKISPNLFT
                     YNTYVSCGYDVNDPEQHAINFSIQSYFDPLTDNAVDYLKSYLKEYNGYNLFNTTTLQI
                     ENAKGIIVSMNLNAGLKSNPDKTPFTLYRQDRNNFYFKSNFDVRKELISDIYQRFYSN
                     DPDMILPFFDKWIFSYAGSVYYSILMASNYLELQPERIFVMENEGDIFVSDLRYYFAN
                     LCMKRNPNKHCL"
     gene            complement(1518..2579)
                     /locus_tag="I9760_002534"
     CDS             complement(1518..2579)
                     /locus_tag="I9760_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HAT5044284.1"
                     /translation="MLKGLKVIDLSSVLAGPSVGTFFAELGASVVKIEHPVHKDVTRT
                     WKLPGEDPDSEISAYFASVNFGKEYLFLDLMKQEDYLCFLNLIAESDILIMNFKKGDD
                     VKLRIQDDHLHSINSRLIIGKINGYGDESDRVAYDLVLQGESGFMSINGTQESGPVKM
                     PVALIDVLAAHHLKEAILLALYQREKSGKGRVVSVSLYDAAVSSLINQASNYLMTGQV
                     PQRIGSLHPNIAPYGELFQTSDGALIILAIGSDRHFVKLCNILGLKDLPGKDEFRHNQ
                     NRVRNREQLAVLIAEQTEKLQSSELLEMLHRENIPAGKIMDLKDVFDNERAKSLIRKE
                     VIDDVPTARVTSVVFKQTL"
     gene            complement(2714..4123)
                     /locus_tag="I9760_002535"
     CDS             complement(2714..4123)
                     /locus_tag="I9760_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAT5044285.1"
                     /translation="MISKKITQISFSLALALIVGVAEAKTTRPADEQQRARSIYKQFI
                     EIQSGFTTGSTTPLVEVAVKYLKKAGFSNEDIFVGGASPQKANLVVRYRGTGDKKPLL
                     LLAHTDVVEAKASDWSMDPFQLTEKEGYFYGRGTLDDKAQAAIWIANLIQYKQEGFKP
                     KRDIIVALTADEEGSSPYNGISWLIKNHKDLIEADFALNEGGWGDLANGKKISQNIQV
                     SEKYIVNYNLEVRNKGGHSSLPTKDNAIYRLAGALERLSKFNFPLKTNDVTAAYFKQM
                     AAIETGPLKTEMVEASKGSKQAMQRLADSSPQWNAILRTTCTPTLLEGGHAMNALPQL
                     AAVTINCRVLPEDSPEMVEQSLKTAINDPEVTLKRIGKLSRGPSSPLSPEILKTITQL
                     TQRYWPEVPTIPIMVTGATDGRYLRSVGIPTYGVMGLFLDRDDFRAHGRDERISVESF
                     YEAHAFLYDLVKQLSSNVT"
     gene            4300..5241
                     /locus_tag="I9760_002536"
     CDS             4300..5241
                     /locus_tag="I9760_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT5044286.1"
                     /translation="MYKIHHYFLFGVLVPFISLVSLFTIFSSDFLTLTTWASILASAS
                     DIGIIVLGVTLLLIGGEFDLSVGANFALSGLLFGSLTLLGINSFLALTIALAIGACIG
                     LINAACTVFFKIPSFIVTLGSMLILRSLILLYTQGLPVSVEDTNGVMQVLAGELSQNL
                     LSSFIWWFAIGFYLIYLLNWTKLGNHIVATGNNCDSAYLSGINTIRVKFFCFVCCGII
                     AAFSGVTQFSHLNSLSPIAGEQYELYAIAATVIGGTSLKGGQGSIISTLLATLLLSTV
                     DTGIVQIGVSTYWYRSFIGLILIFAMTINVYLRKSDS"
     gene            5238..5978
                     /locus_tag="I9760_002537"
     CDS             5238..5978
                     /locus_tag="I9760_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAT5044287.1"
                     /translation="MINSHQTQPLLRLENIEKSYGSHRILNKVSFCLYPGEITALIGD
                     NGAGKSTLVQIISGYIQADKGNIYWQEQPLCYSNQYNAPFEMRRLGIQIVHQQLALID
                     ELSIWRNFFLGNEINKKIGPFKFLNIRLMQHIASQSLQSFGLVCLPNIDLPVKKLSGG
                     ERQMLVVCRAKYFNAKLLILDEPTSALSPAQADKVLQIIKNIAQAGIAIVFITHNPAH
                     IQNTAHKVIKLSCGTIEIMELTNTLIKG"
     gene            5982..6866
                     /locus_tag="I9760_002538"
     CDS             5982..6866
                     /locus_tag="I9760_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAT5044288.1"
                     /translation="MKKLASTLLCFSLLISASVFARDKYYLITHGSQDPYWTSLFQGA
                     KKAAEELKVDLQILAPPGANDVPKQVQFIESALATYPSGIATTIPSDTAFSKSLQRAN
                     KLNIPVIAVDTRPKDKTKNPYLVFLGSDNLLAGKKLGEKALELTPSAKRALVLNPQPG
                     HIGLEKRAYGIKTILQDKGIFFEELDVGTDPNQVQSRVKSYFKIHPETNIIFCLTSQA
                     LDPLGQMLLHPDRYDFNYQPQVYSFDKTPNTVSLIHKKLVNYVMDQQPFLMGYLSITQ
                     LVLMNRYQLNPVNINTAM"
BASE COUNT         2134 a         1445 c         1263 g         2252 t
ORIGIN      
        1 ccagagcatt gggtataatt tgcgcatcac aacgtaaaag attttgataa cttgttgtaa
       61 taatattttg cagctcattt aagtgttttt catacttgtt gagtaaagca agtgcttctt
      121 tttgggatat catatttttt cctcaggttt tcacatggct cgaaattcta acagtaaatt
      181 attaaatgaa tattaaatta attttgaccc aagccattgt gttcttttat aaactattac
      241 attcatttgc ttcttggtat tgcccatcca ttcaaaacca tatacttctc aatagttatc
      301 tatggctttt taaagtaaaa aaatttgcta ttccctgtcg ctcacatgcg ctcatcgcat
      361 ctgttttatt tatgctggtt ataggatcta atcttcaaac cagataagat atactgtctg
      421 tcccatccga ttagaacaat tattaaacag tattctaatc ggataatcat ttgaatggag
      481 ttctgaatta atttaaaaag gagtttgtta tgaaaccagg ttatttatta ggcgctttgc
      541 ttttatctcc cattcttgct tttgccaatt ctgataacaa cacagatggc cttatttttt
      601 cgccattgcc gcagaataaa aataccgtag tcaggcacta tagcaatgaa caggaaatgc
      661 ctaatcttag tcaaatggct caaagaacta ttgattttcc aactcaaatt gttcgagtca
      721 gcggtaatct tacaggattg gaactgagct gtgatgatgt agaaaatgaa atagatcaag
      781 ttttttcaaa aaaaatctcc cctaatctgt ttacttacaa cacttacgtc agctgtggct
      841 atgatgtcaa tgatccggaa cagcacgcga taaacttctc tattcaaagt tatttcgatc
      901 cattgacaga taacgctgtt gattatttaa aatcctatct gaaagaatat aatggataca
      961 atttattcaa tacgacaaca ttacaaattg aaaatgccaa aggcatcatt gtatcgatga
     1021 atcttaatgc cggtttaaaa agtaaccccg ataaaacacc attcacgctt tatcgtcagg
     1081 ataggaacaa tttttatttt aaaagcaatt ttgacgtgag aaaggaatta attagcgata
     1141 tttatcaacg attttattct aacgatcctg atatgattct accctttttc gacaaatgga
     1201 ttttctccta tgccggatca gtttattact ccatccttat ggcatccaat taccttgaat
     1261 tgcaacctga aagaattttc gtaatggaaa atgaagggga tatcttcgta tccgatttac
     1321 ggtattactt tgccaatctt tgtatgaaga gaaatcctaa taaacattgt ttgtaactaa
     1381 tgaaatcagc aaaaaccgtt tagcgcgtat gctttctaat gtgcgctgca gagacgtttg
     1441 cagcgcagca atgaagagac agcttatgca tgaggactaa tattattcat ctgcataatt
     1501 caatggctcc cctttagtta taaagtctgt ttaaaaacaa cggatgtaac gcgcgctgtg
     1561 ggaacatcat caataacttc cttgcgaatg agggattttg cacgctcatt atcgaaaacg
     1621 tccttgagat ccataatttt gcctgcagga atattttccc tgtgcaacat ttcgagaagc
     1681 tcactgctct ggagtttctc tgtttgttca gcaattagta cagcgagttg ctcccgattc
     1741 ctcaccctgt tttgattgtg cctgaattcg tcttttcctg gtaaatcttt tagcccaagg
     1801 atattgcata gttttacaaa atgcctatct gagcctattg ccagtatgat aagcgcccca
     1861 tcagatgttt ggaacaattc gccatagggg gcaatattgg gatgcagtga tccgatgcgc
     1921 tgcggcacct gcccagtcat caagtaattc gatgcctgat tgataagact gctaacagca
     1981 gcatcataaa gggaaacaga gacaaccctg cctttcccgc ttttttcccg ctgatagaga
     2041 gccagaagga tggcttcttt cagatgatgg gctgcaagga catcgattaa ggcgacgggc
     2101 atttttaccg ggccgctctc ctgtgtccca ttgattgaca taaaaccgct ttcaccctga
     2161 agcacaagat cataggcaac gcgatcgctt tcgtcaccgt agccattgat ttttccaatg
     2221 ataaggcgcg aattaatact gtgtagatga tcatcttgta tacgcagctt tacatcatct
     2281 cccttcttaa aattcatgat taaaatatca gattctgcga tgagattaag gaaacagaga
     2341 taatcctctt gcttcatgag atctaaaaac aaatattcct ttccaaaatt aacactagca
     2401 aaataagctg aaatctcaga gtcaggatct tctccaggga gtttccatgt tcttgtcaca
     2461 tccttatgta cgggatgttc gattttaaca actgatgcac ccaactcagc aaaaaaagta
     2521 cctacagagg ggcctgcaag aaccgaactc agatcaatga cctttaagcc ctttaaaata
     2581 tacctgtttt ccatatctca ataaacattc gttgttttta caagctttca cattgggaaa
     2641 ttgcgcaaaa ctgacccaag ggcctcaaat caatatccta cctttagcaa agccccgact
     2701 ggaaaaacca ttcttaagtt acattgcttg acagttgttt caccaaatca taaaggaatg
     2761 catgagcttc ataaaaggat tcgacactaa tccgttcatc acgaccatgg gctcggaaat
     2821 catcgcgatc taaaaatagc cccatcaccc cataggtagg aattccaact gatcgcagat
     2881 agcgcccatc cgttgcacca gtaaccataa ttggaatggt aggaacttca ggccaatacc
     2941 tttgagtgag ctgagtaatt gtttttaata tttcaggact aagtggagag ctcggacctc
     3001 tggacaattt gccaatgcgc tttagtgtaa cctctggatc gttaatagct gtcttcaaag
     3061 attgctcaac catttcaggt gaatcttctg ggagcacacg acaatttatg gtaacagccg
     3121 ctaattgtgg taatgcattc attgcatgcc ctccttcaag taaggtaggg gtgcaggttg
     3181 ttcgtaaaat ggcattccat tgcggtgaac tatcagctaa acgttgcata gcttgcttcg
     3241 agcctttgct tgcttcaacc atttctgttt tcagaggccc tgtctcaata gccgccattt
     3301 gtttaaaata ggccgcagtt acatcattgg tttttaaagg aaaattaaat tttgacagtc
     3361 gctctaaagc ccctgcaaga cgataaatgg cattatcttt ggttggcagc gaactatgcc
     3421 cccctttatt acgaacctca aggttgtaat tgacaatgta tttttcactc acctggatat
     3481 tttgtgatat ttttttccca ttggccaaat caccccatcc accctcatta agagcgaaat
     3541 ctgcctcgat taaatccttg tgatttttaa tcagccaact aatcccgttg tatgggctgc
     3601 ttccttcttc atcagccgtc aaggcaacaa tgatatcgcg tttgggttta aatccttcct
     3661 gcttatattg gattaagttg gctatccaaa tagccgcttg cgctttatca tccaaagtgc
     3721 ctctgccata aaaataccct tctttttcag tcagttggaa aggatccatg ctccaatcgg
     3781 aagctttggc ttcaacaacg tccgtatgcg caagtaataa caagggtttc ttgtccccag
     3841 taccgcgata acgtacaacc agattggctt tttgtggact ggctccacca acgaaaatgt
     3901 cctcattgga aaaaccagct ttctttaagt actttactgc cacctcaacc aacggcgttg
     3961 ttgaaccggt agtaaatcct gattgtattt cgataaattg tttataaata gagcgagcac
     4021 gttgttgttc atccgcaggg cgagtagtct tcgcttctgc caccccaacg atcagagcca
     4081 gagcaaggga aaatgaaatt tgagttattt ttttcgaaat cataaaatga aatccattca
     4141 aagcatgacg cgtttaaaga gaaatccact ataaagtaat ctgagtaaaa atcaatggta
     4201 cccgggttga acttgccttc aatgttctct gaaaattgtg cttaaagatc gataatattt
     4261 tgtgtacact ttccacaata aatttccagg gatagtctca tgtataaaat acaccattat
     4321 tttttatttg gtgttctagt tccatttatt agccttgtta gtctattcac tattttctct
     4381 tctgactttt taacccttac tacttgggca agtattttgg catccgcctc tgatattggc
     4441 atcattgtcc ttggagttac tcttctgctt ataggaggag agtttgacct ctctgttgga
     4501 gcgaattttg ctttatctgg cttgctattc ggttcactta cgttattagg aatcaatagt
     4561 tttttagctt taacaattgc tttggcaata ggagcctgta taggattaat taatgccgca
     4621 tgcaccgtct tttttaaaat cccctcattt attgtaactt tgggaagcat gttaatttta
     4681 cgtagtctta tccttttgta cacccaaggt ttgcctgtat ctgttgagga cacaaatggt
     4741 gttatgcaag tattagcagg tgaattatca caaaatttat tgtcttcttt tatttggtgg
     4801 tttgctattg gtttttatct aatctatctt cttaattgga caaaactggg gaatcatata
     4861 gttgccactg gtaacaattg tgattccgct tatctatctg gcataaatac aattcgagtt
     4921 aaattctttt gttttgtgtg ttgtggaata atagccgcat tttccggagt aactcaattt
     4981 agccacctta atagtttatc ccccattgcg ggtgaacaat atgaattata tgccatagca
     5041 gctaccgtaa tcggtggtac ctcactaaaa ggaggtcaag gatccataat aagtacgcta
     5101 ttggctactt tattattaag cactgtagat accggcatag tacaaattgg tgtttcaact
     5161 tattggtatc gatcttttat tggacttatt ttaatctttg cgatgactat taatgtttat
     5221 ctgaggaaat ctgactcatg ataaatagcc atcaaactca acctttactc aggttagaaa
     5281 atattgaaaa aagttacggc tcccatcgta ttctcaataa agtatctttt tgcttatatc
     5341 ccggggaaat tactgcttta atcggtgata atggagcagg taaatcaacc ctggtacaaa
     5401 tcatttcagg ttatattcaa gcagataaag gaaacattta ttggcaggag cagcccttat
     5461 gttatagcaa ccaatataat gctccatttg aaatgagacg cctgggtata cagattgtcc
     5521 atcaacaatt agcgcttatt gatgaattat ctatatggcg taatttcttt ttaggtaatg
     5581 aaataaataa aaaaataggt cctttcaaat tcttaaacat caggcttatg caacatatag
     5641 caagtcagtc cctgcaaagc tttgggcttg tatgccttcc aaatattgat ctgccggtca
     5701 aaaaactatc tggtggtgaa cgccaaatgc tagtcgtttg tcgcgcaaaa tattttaatg
     5761 caaagctact tattcttgac gagccaacca gtgccctctc acctgcgcaa gcagataaag
     5821 tccttcagat cataaaaaat atagctcaag ccggaattgc aattgttttt ataactcata
     5881 atccagctca tatacaaaat accgcacaca aagtgatcaa gcttagttgc ggaacaatag
     5941 aaataatgga attaactaac acgctcatta agggttaaac tatgaaaaaa ttggcatcaa
     6001 ctctcttgtg tttctcgcta ctgataagcg cctctgtctt tgcgagagat aaatattatt
     6061 tgataaccca tggttcacaa gatccttatt ggacatcttt attccaggga gcaaaaaaag
     6121 ctgctgaaga actgaaagtc gatttacaaa ttttagctcc tccaggagcc aatgatgtac
     6181 ccaaacaggt tcaattcata gagagcgcac tggctaccta tccttctgga attgctacta
     6241 ccattccttc agacacagct tttagcaaaa gcctgcaaag ggcaaataaa cttaatattc
     6301 ctgttattgc cgttgatacc agaccaaaag acaaaacaaa aaatccttac ctggtctttt
     6361 taggttctga taacctactc gcaggcaaga aacttggtga aaaagctctg gaattaactc
     6421 caagcgctaa aagagcacta gttcttaacc cacaacccgg ccatataggg ctcgaaaagc
     6481 gtgcctatgg aattaagacc attcttcaag ataaaggaat cttttttgag gaacttgatg
     6541 taggtactga tcctaatcaa gtacaatcca gagtcaaatc ttactttaag atacatccag
     6601 aaacgaatat tattttttgc ctaacctcac aagcactaga tccacttgga caaatgttat
     6661 tacaccccga caggtatgat tttaattatc aacctcaagt ctattccttt gataaaacac
     6721 ccaatactgt ttctttaatt cataaaaaac tggtgaatta tgtaatggat caacaaccat
     6781 ttctaatggg ttatttaagt atcacccaac tggtattgat gaaccgatat caattgaatc
     6841 cagtaaatat caatactgca atgtaaattg cagtatatgt gttatattcc agccaatgac
     6901 ttgttgcttt ttgatagtgt catgggtaat ttcgaacgct tccaggaaac cacttgcaat
     6961 caaaatcgca agaaaaatta agtacaaacc attctatatt ggaatcacat atgagcgatt
     7021 agtttaagga tgcaaactca tttggcacca atatcgccct cagtttgtct ttgagagcga
     7081 tattaattta ataa
//
LOCUS       DACTJE010000164         7062 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.165, whole genome shotgun sequence.
ACCESSION   DACTJE010000164 DACTJE010000000
VERSION     DACTJE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.165"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..814
                     /locus_tag="I9760_002539"
     CDS             <1..814
                     /locus_tag="I9760_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5044289.1"
                     /translation="QARKENKSYAIYSPEMEEDLINNRIVMNELQRSIENKDLKIYYQ
                     PIVELANGQIVGAESSARFEHPELGLLSAEKFMPLIEGTSLIHNLTTLMLKEVIKQLA
                     AWHDAGHKIFVSANLSVNRGLPDLIEKLLNDYEIAPQFLKLEFTERTCLAEQMVTSEV
                     FERLSTMGIKLCIDDFCSENSSFIYLTDFPIENIKVEKSFVLKIAKDAKKAKIVEAIV
                     KLAQTLGWEPLADGIADQNALEKLRDLGCLYGQGLYFSRAVNVAEFTSLLKK"
     gene            complement(906..2477)
                     /locus_tag="I9760_002540"
     CDS             complement(906..2477)
                     /locus_tag="I9760_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HAT5044290.1"
                     /translation="MRRQWYIGFGVILLCLFALFAMLQDIVWFFAFLIPIVILWIYDV
                     LQTKHTILRNFPVLGHVRYFLEFLRPEIQQYFIATDESELPFNRETRSMIYQRAKNVR
                     DTIPFGTERDILSVGYTWALHSLSPKHSSEVEPRIDVGGPDCKKPYNASRLNVSAMSF
                     GSLSANAIMALNKGAKLGGFYHNTGEGGLSPYHLQGGDIVFQIGTAYFGCRDDKGDFD
                     ENEFIKEASRDEVKMIEIKLSQGAKPSHGGILPAAKLTEEIAKVRKVPMGKDVLSPIA
                     HTAFDNPVGLLHFVKRLRDLSGGKPIGFKLCLGRRHEFMAICKAMLKTNILPDFITID
                     GAEGGTGAAPVEYTNFIGTPLEAGLVFVHNALIGTGVRDKIRVICSGKVTNGFDLLTN
                     IALGADICNSARAMMMAIGCIQSKQCNANTCPTGVATQKKRLQYGLVVDQKKFLVANF
                     HNNTIKSFLEMVGALGLNNPSDLKPSHIMRRIGVDEVKSFDQIYTYVKPGQFLQGDIP
                     EDYRIHWEHANPDKF"
     gene            complement(2555..2869)
                     /locus_tag="I9760_002541"
     CDS             complement(2555..2869)
                     /locus_tag="I9760_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAT5044291.1"
                     /translation="MTNIKLCKIKLLNKIYEIKCPDGEEVSLILAAEKLNEKMQNNKN
                     KFKHLDNFHILLLAALDIGHELIVCKNEQEQQRLQVTQFISSLENKINKAVVGSETDS
                     NP"
     gene            3025..3606
                     /locus_tag="I9760_002542"
     CDS             3025..3606
                     /locus_tag="I9760_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HAT5044292.1"
                     /translation="MSLDNDSLHLPKYDDFVQSISVLALTMSGSELHGIMCGYLCAGA
                     DSQGEAYIRALLNNKKDEQSRNALLSMFSVFSISQQQMNNFDFEFEMLLPDDDESLVT
                     RAQAFSEWCEGFTQGLTIAGVGMEQFYEEESQDALQHLMEFAELDCESLEVGEEDERA
                     LMEVSEYTRMAVLRLHSDLVLHERELGDSGTTH"
     gene            3711..5021
                     /gene="pepP"
                     /locus_tag="I9760_002543"
     CDS             3711..5021
                     /gene="pepP"
                     /locus_tag="I9760_002543"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAT5044293.1"
                     /translation="MISQQEYKARRKKLAAQLPDDSVAVIPAAHEVLRSGDTHYRFRQ
                     DSDFYYLTGFNEPDALLVIIAGKDSQSILFNRPRNPAEEQWTGKRLGQDGALQELGVQ
                     AAYPLSSITEELPKLFSGKSAIYYDLGRNLSLEKKIRHVLSSLKAQVRRGIKAPESLC
                     DLEPILSEMRLLKSDAELELMRQAARISVAAHKRAMQVCQKMEYEYQLEAELIYEFSR
                     HGCRSVAYDPIVGGGANACILHYTENNKPLRRGDLVLIDAGGEYGSYAADITRTFPVS
                     GTFSPEQKIIYELVLKAQKAGIAAIEPGLPWNHIQQTIIRILTEGLCDIGILKGDLEE
                     LIKNEAYKPFYMHNSGHWLGLDVHDVGRYKINNEWRPLEAGMVLTVEPGLYISAGMEG
                     VDKRWWDIGVRIEDDILVTRAGYEVLTGDLPVNVDDIEALMRGE"
     gene            5011..6213
                     /gene="ubiH"
                     /locus_tag="I9760_002544"
                     /gene_synonym="visB"
     CDS             5011..6213
                     /gene="ubiH"
                     /locus_tag="I9760_002544"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAT5044294.1"
                     /translation="MANKETDILIIGGGLTGASLSLALQGLGFSVTLVESRPYSDKVN
                     PDFDARSLALSPASQRILAMLGVWEILKEYTTPIHMIHVSDQHRFGASRLHGEKNSPL
                     GYVVEMQYINRALSILINKEHLLTPAALKSIDFNNSTATVAHGDDMISIRAQLIVAAD
                     GTESMTRYLCNLPVKIKDYAQDAIVANVGLIKPHGNRAFERFTPNGPLALLPMMNDKM
                     SLVWASKPEEAKRLVMLNDDDFLRELQTAFGYRLGRFGKVGKRYSYPLKQVLMPQQTK
                     WPVVFVGNAAHTLHPVAGQGFNLGLRDIAALAQCISQKGLNPQMLEHYLQLRAHDQQM
                     ITWLTDGLIHLFTSRLPGMGVARNLGLLALDNIPALKNLLARYARGFGGITPDLVCEI
                     ALSAGEFQ"
     gene            6210..>7062
                     /locus_tag="I9760_002545"
     CDS             6210..>7062
                     /locus_tag="I9760_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT5044295.1"
                     /translation="MSQHFDVLVIGGGIVGLTAALAMAQRHYTVAVIDAGTLMVNTDK
                     SDLRVYAINKASKMLLSELEVWQHLNDARVAPYNKMHVWDGVSGAYIDFDSRSIAEPN
                     LGAIIEESILKQALLRRISSESKISLFPQSVIDEVCHLERSIKISGQNTAWEGQLLMI
                     ADGANSPMRHKLKVDVTSWSYEQQALVATVSSEKSHQNTAFQVFHPDGPLAFLPLSNL
                     NQCSIVWSTHADNAMRLKLLDDNEFSRELTKAFGGQLGQIKVVSSRHQFPLQMRHVKQ
                     YAGHRWLL"
BASE COUNT         2061 a         1277 c         1588 g         2136 t
ORIGIN      
        1 ccaggcacga aaggaaaaca aatcctatgc aatttacagc cctgaaatgg aagaagacct
       61 tattaataat cggattgtga tgaatgaact gcaacgctcc attgaaaaca aagacttgaa
      121 aatatattac caaccgatag ttgaattagc taatggccaa attgtaggtg ccgagtcatc
      181 agctcgtttt gaacatcctg aattaggttt attaagcgcg gaaaaattca tgcctttaat
      241 cgagggaacc agtttaattc ataatttgac aactcttatg cttaaggaag tcataaagca
      301 attagccgct tggcatgatg ctggacataa aatttttgtc tcggcgaacc tgtcagtgaa
      361 tcgtgggctg cctgatttga tagagaaatt attaaatgat tatgagattg ccccacaatt
      421 cctgaaactt gaatttaccg aaaggacttg tttagctgag cagatggtta ccagtgaagt
      481 ttttgaacga ttatcaacca tgggcatcaa attatgcata gatgattttt gcagcgagaa
      541 ctcttccttt atttatttga ctgatttccc tattgaaaac attaaagttg agaaatcttt
      601 cgtactgaaa atagccaaag acgcaaaaaa agccaaaatt gtagaggcta tagtaaaact
      661 ggcccaaaca ttagggtggg aacccctggc agatggcatc gctgatcaaa atgcattgga
      721 aaaattaaga gacttaggct gcttgtatgg ccagggatta tatttttctc gagcggtcaa
      781 tgtggctgaa tttacatcat tgctaaagaa atgaatctgg ctttaaatca cttaccattc
      841 gttttcccgc tttgattcac atggaatacg aatcacaacg ctgacacgct atggccacac
      901 tgatattaaa acttgtctgg attggcatgc tcccagtgga ttctataatc ttcaggaata
      961 tcgccttgta aaaattggcc tggcttaacg taagtataga tttgatcaaa agatttgact
     1021 tcatccaccc cgattcgtct cataatatgg gatggtttta aatcactggg attgtttaat
     1081 cccaaggcgc caaccatttc caaaaagctt tttatcgtat tattatggaa attagcaaca
     1141 agaaattttt tttgatcaac aacaagacca tactgaagac gtttcttttg agtggcaact
     1201 cctgtcgggc aggtattggc attgcattgt tttgattgta tgcaccctat tgccatcatc
     1261 atggctcttg ctgaattgca gatatcggcc ccaagagcaa tattagtgag caaatcaaac
     1321 ccgttggtta cttttccact gcaaattacg cgaattttat cacgtacacc ggtgccaata
     1381 agggcattat gaacaaaaac cagaccggct tccaaaggag ttcctataaa attggtgtat
     1441 tcaaccggtg ctgctcctgt ccctccttcg gcaccatcta ttgtaataaa atcaggtaaa
     1501 atatttgttt tcagcatggc tttgcaaata gccataaatt catgtcggcg cccaaggcag
     1561 agtttaaagc ctatgggttt tccacctgaa agatcgcgta atcgttttac aaaatgaagt
     1621 aaaccaacgg gattatcaaa agcggtatgg gcaattggcg ataaaacatc tttgcccatg
     1681 ggcacttttc ttacttttgc gatttcttca gttaatttgg ctgcaggtaa aattccacca
     1741 tgagatggct tcgcgccttg tgataattta atttctatca ttttgacttc atcacgggat
     1801 gcttctttga taaattcatt ttcgtcaaag tcgcctttgt catcacggca tccaaaataa
     1861 gctgtgccaa tttgaaaaac aatgtcaccg ccttgcaaat ggtaaggact taacccccct
     1921 tcccctgtat tgtggtaaaa gcctccaagt tttgctccct tgtttaatgc cattatggca
     1981 ttagccgata aagagccaaa gctcatggct gatacgttca atcgggaggc attgtaaggt
     2041 tttttacaat caggtccacc cacatcgatt cttggttcca cttcgctaga gtgtttcggc
     2101 gatagagaat ggagtgccca agtataaccg acgcttagaa tgtctcgctc cgttccgaaa
     2161 ggaatggtgt ctctgacatt ttttgcgcgt tgataaatca tggagcgggt ttccctgtta
     2221 aaaggaagct cgctttcatc cgtagcgatg aaatattgtt gaatctcagg gcgaagaaat
     2281 tccaaaaaat atctaacgtg acctagaact ggaaaattgc gtaaaatggt gtgttttgtt
     2341 tgcaatacat cataaatcca caggattaca ataggtatca aaaaagcaaa aaaccagaca
     2401 atatcctgca gcatggcaaa cagggcaaag agacataaaa ggataactcc aaatccgata
     2461 taccattgac gtcgcatagc taattccttt taattgtttg ctattaatat caatagtata
     2521 ggatggtttg ccgaggaata cttaatttat ctgcttatgg attgctatcc gtttcagagc
     2581 caactactgc tttattaatt ttattttcca aagaagatat aaattgggtc acctgcaatc
     2641 gctgttgctc ctgttcgttt ttacatacaa ttaattcatg gccaatgtcc agagcagcaa
     2701 gtaaaaggat gtgaaaatta tccaggtgtt taaatttatt tttgttattt tgcatttttt
     2761 cattaagttt ttctgctgcg agtattaaac tcacttcttc gccatcaggg catttgattt
     2821 catagatttt atttaataat tttattttac agagtttaat attggtcatt attacgcctt
     2881 tgtatttgca cggtattatg caaatagtag caattttgcc ttaatgcagc aaattatgaa
     2941 aatcataggt gctgagaaag tgcagcaggt gaacactgat tctaaatgga gtatcattag
     3001 ctatcttttt aaatagggtt aaatatgtct ttagataatg attcgctgca tttacctaag
     3061 tacgatgatt ttgtgcaatc catttctgtt ttagcattaa ctatgtccgg aagcgaatta
     3121 cacggaatca tgtgcggtta tctgtgtgca ggggctgata gtcaaggcga ggcttacata
     3181 cgcgctttgc taaataataa aaaagatgag caaagtcgta atgcgttatt atccatgttt
     3241 tctgtctttt caattagcca gcaacaaatg aataattttg attttgaatt tgaaatgtta
     3301 ttgcctgatg acgacgaatc tttagtcact cgtgctcagg cttttagtga gtggtgtgaa
     3361 ggctttacgc aagggttaac tattgcagga gttgggatgg agcagtttta tgaggaggag
     3421 tctcaggatg ctcttcagca ccttatggaa tttgcagagc tggattgtga gtctttggag
     3481 gtaggcgagg aagatgagag agcgttgatg gaggtcagtg aatacactcg tatggcagta
     3541 cttcgattgc acagtgattt ggttttgcac gaaagagaac ttggagattc tggaacaacc
     3601 cattgatagc aagttgataa agtatcgagg ttgtttgtat ttattggttt ctttgatttt
     3661 ttaaggattg ctcatttgtt ctatttatta aagtgaaaag ggaaattgat atgataagcc
     3721 aacaagaata taaggcgaga agaaaaaaat tagctgcgca attgcctgat gacagtgttg
     3781 ctgtaatacc tgcagcacat gaagtgctgc gcagtggaga tactcattat cgatttcgac
     3841 aggacagtga tttttattat ttaacaggat ttaatgagcc tgatgctttg ctggtcatta
     3901 ttgcgggaaa ggatagccaa agtattttgt ttaatcgccc aagaaatcct gcagaagaac
     3961 aatggacagg taaacgatta ggtcaggacg gcgctttgca agagcttgga gtgcaggcgg
     4021 catacccatt aagcagtatt acagaagagc ttcctaaact attcagcggg aaatctgcca
     4081 tatattatga cctcggccgt aatttatcat tggaaaaaaa aatcaggcat gttttatctt
     4141 cactaaaagc ccaggtgaga agaggcatta aggcgcctga gtccttatgc gatctcgaac
     4201 ctattctaag cgaaatgcgt ttgcttaaaa gcgatgcgga attagagtta atgagacagg
     4261 cagcccgtat ttcagtggct gctcataaac gagcgatgca agtttgtcag aaaatggaat
     4321 atgagtacca actggaggca gaattaatct atgagtttag ccgtcatggc tgtcgtagtg
     4381 tagcttatga ccctatcgtg ggtggtggcg ccaatgcctg cattcttcat tatacagaaa
     4441 ataacaaacc tttgcgccgt ggcgatttgg tgctcattga tgcagggggt gaatatggta
     4501 gttatgccgc agacattacc agaactttcc cggtaagtgg tacttttagc cctgagcaga
     4561 aaatcattta tgaattggta ttgaaagcac aaaaggctgg catagcagcg attgaaccgg
     4621 gtcttccctg gaatcatatt cagcagacta ttatcagaat tcttactgag ggtctctgtg
     4681 atataggaat tttaaagggc gatttggagg agctcattaa aaatgaagcc tataaaccat
     4741 tttatatgca taattcagga cattggttag gattggatgt gcatgatgtc gggcgatata
     4801 aaatcaataa tgaatggcgt cctttagagg ctggaatggt attaacagta gaaccagggt
     4861 tgtatatcag cgcagggatg gaaggcgttg ataaacgatg gtgggatatc ggtgtgcgta
     4921 tagaagatga tattctggta accagggcag gatatgaggt attgaccggg gacttgcctg
     4981 ttaatgtaga tgatattgag gctttgatgc gtggcgaata aagagactga tatactgatt
     5041 attggcggtg gtttgactgg tgcaagctta tccttggctc tgcagggatt aggtttctct
     5101 gtcacgttag tagaatccag gccatacagc gataaggtaa atcctgattt tgatgctcgc
     5161 tcattggcgt tgtcgcctgc aagtcaacgt atattggcaa tgctgggagt ttgggaaatt
     5221 ctcaaagaat atacaacacc aattcatatg attcatgttt ctgatcagca tcgctttggg
     5281 gcttctcgtt tacatggcga aaaaaacagt cctttgggct atgtggtaga aatgcaatac
     5341 atcaacaggg ctttgagtat tctaatcaat aaagaacact tgttaactcc agcggcttta
     5401 aaatccatcg attttaacaa ttctactgcc actgtcgccc atggcgacga tatgatttct
     5461 atccgagctc aattaattgt cgcagccgat gggacggagt cgatgacacg ctacctttgt
     5521 aatctgccag tcaaaataaa agattatgct caagacgcta ttgtcgccaa tgtaggtctg
     5581 ataaaaccgc atggcaatcg tgcttttgaa cggtttacgc ctaatggtcc tcttgcatta
     5641 ttgcctatga tgaatgacaa aatgtcattg gtatgggcat caaagcctga agaagcaaaa
     5701 cgcttagtga tgttgaatga tgatgatttt ttgcgagaac tgcaaactgc ttttgggtat
     5761 aggttagggc gatttggtaa agtaggaaag cgatactcct atcccttgaa acaagtttta
     5821 atgccccagc aaaccaaatg gcctgttgtt tttgttggta atgccgctca cactttgcat
     5881 ccagttgcgg gtcaggggtt taacttgggt ttaagggata ttgcggcttt ggcacaatgc
     5941 atcagccaaa agggattaaa tccacagatg ttggagcatt atttgcaatt acgagcacat
     6001 gatcagcaaa tgattacctg gctaacagac ggtttaatac atctatttac gagtcgttta
     6061 cccggtatgg gagtagcgcg taaccttgga ttgctggcac ttgataatat accggcatta
     6121 aaaaatttat tagcgcgata tgcgcggggg tttggcggaa ttactcctga tttggtttgt
     6181 gaaattgcat taagcgcagg agaatttcaa tgagccaaca ttttgatgta cttgtcattg
     6241 ggggcgggat tgttggcttg acagcagctt tagctatggc ccaacgccat tatactgttg
     6301 ctgttatcga tgcagggaca ttgatggtca atacagacaa gagtgatttg cgtgtttatg
     6361 ccatcaataa ggcatcaaaa atgttattaa gtgaattaga agtgtggcag cacctcaatg
     6421 atgcccgggt cgctccttat aataaaatgc atgtatggga tggtgtgagt ggcgcatata
     6481 ttgattttga ttctcgcagt attgctgagc caaatttagg ggcaattatt gaagaatcaa
     6541 tcttaaagca agctttgttg cgtcgaatct catcagaatc caaaataagt ttatttcctc
     6601 aaagtgtaat agatgaggtg tgccatttag agcggagtat caagatctcc ggccaaaata
     6661 ctgcctggga agggcagtta ttgatgatcg ctgacggagc taactcccct atgcgtcata
     6721 agcttaaggt ggatgtaacc agctggtctt atgagcagca agcgttggtc gcaacggtaa
     6781 gtagtgagaa atcacatcag aatactgctt ttcaagtgtt tcatcctgat ggccctttgg
     6841 cttttttgcc attatctaat ctcaatcaat gttctatcgt ttggtcaact catgccgata
     6901 atgcgatgcg attgaaatta cttgatgata atgaatttag ccgggaattg actaaggcat
     6961 ttggaggtca gctagggcaa ataaaggtag taagcagtcg ccatcaattt cctttacaga
     7021 tgagacatgt caagcaatat gcagggcatc gctggttatt gc
//
LOCUS       DACTJE010000165         7012 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.166, whole genome shotgun sequence.
ACCESSION   DACTJE010000165 DACTJE010000000
VERSION     DACTJE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.166"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..608)
                     /locus_tag="I9760_002546"
     CDS             complement(<1..608)
                     /locus_tag="I9760_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAT5044296.1"
                     /translation="MSKELLLVAEALSNERGVSKDVIILAIQAALESATRKILGLDIG
                     VRIKIDSKTGDYETFRYWDVVNEEELEFPDRQLSLEQAKERKPSAAVGDRIEEIIPSI
                     EFGRIEAQTARQVIMQKVREAERELIIDQFRSKLGQLIYGTVKKVTRDNIIIDLGGKA
                     EAFLPRSEMLPHEMFRPNDRVRAYLYEITPQARGPQLFVSRTR"
     gene            complement(620..1063)
                     /gene="rimP"
                     /locus_tag="I9760_002547"
     CDS             complement(620..1063)
                     /gene="rimP"
                     /locus_tag="I9760_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAT5044297.1"
                     /translation="MINDDLIVLLEPIIKNMGYELWGCEYLSQGKHSLLRIYIDKPDG
                     IGIDDCQEVSKQVSAMLDVEDPIPGHYSLEISSPGIPRPLFSIWQYQRYLGYEIHVKT
                     FKPVNGKRKLSGIIVSASEDTIVLDINNEHQEILLSNIVKANLTV"
     gene            complement(1177..1253)
                     /locus_tag="I9760_002548"
     tRNA            complement(1177..1253)
                     /locus_tag="I9760_002548"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1217..1219),aa:Met,seq:cat)
     gene            complement(1319..2761)
                     /gene="nuoN"
                     /locus_tag="I9760_002549"
     CDS             complement(1319..2761)
                     /gene="nuoN"
                     /locus_tag="I9760_002549"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAT5044298.1"
                     /translation="MMTLLENIHVAIPEMIILITACLALLSDLFFRHKLKSIAFYISC
                     VGIIVSAAVSFVFIGSYKLLIFNKLFISDDISHLMKLFIDITVLLSFVYSQNYLDERQ
                     MPTGDYYVLGLFSTLGMMILVSAHSLLSLYLGLELMSLPLYAMTAIRRTDSDASEAAM
                     KYFVMGAIASGMLLYGISLVYGATGKLDLLDVANAVAVNWQQQNTLFAFAMVFILAGV
                     GFKLAAVPFHMWAPDVYQGAPSSVTIFISTAPKIAALGMALRFLTIGLVDITMQWQQI
                     ILVMALLSTGIGNLLAVIQTRIKRLLAYSAISHIGYALFGVLAASSAGYAAALYYILV
                     YAVMSAAAFGLIVLMSNHGMEIDSIDDLKGLNKRNPWLAFMMLLVMFSMAGVPPTVGF
                     FTKLLVLKALVDVHMTWLAVVGLLFTVIGAYYYLRIVKIMYFDQPENEEIVRIGTGNT
                     IVYSLNCLSLLYLGIFPGALIAACINAFAN"
     gene            complement(2815..4320)
                     /locus_tag="I9760_002550"
     CDS             complement(2815..4320)
                     /locus_tag="I9760_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAT5044299.1"
                     /translation="MHYLLNLLIWLPILGGVFVLLTGDDKNPNVSRYLTLFTILLCLV
                     LCIPLVKGFDLQSFGMQFIEEIPWMPALGINYTLGIDGLSLLLIVLSIFTNLIVILAT
                     WDSISKKVSQYLSAFLIMQGLLVGIFSAMDAIVFYIFWEATLIPMYLIIGIWGSDNRV
                     YAAIKFFLYTFLGSVLMLASFLYMGYIAGSFKIETFYAIKLGMTAQTLIFIAFFLGFA
                     IKIPMFPVHTWLPDAHTEAPAGGSVVLAAILLKLGAYGFIRFSLPIVPDACSKYAPLM
                     VALSLIAVVYIGLIAIIQKDMKKLIAYSSISHMGFVTLGCFAIFAIARHGGLSNAGLI
                     LEGAIVVMISHAFVSSAMFAGVGFIYDRMHTRQLADFGGIVNTMPIFASFFMLFAMAN
                     AGLPGTSGFVGEFMVILGSIKAGFWVAFWAATTLIIGAAYTLWMYKRVIFGPVANKKV
                     AALKDISGFELSAYVLLAIMVIALGVYPKPMLEFVHQTVSHTLALADKSKL"
     gene            complement(4336..6309)
                     /gene="nuoL"
                     /locus_tag="I9760_002551"
     CDS             complement(4336..6309)
                     /gene="nuoL"
                     /locus_tag="I9760_002551"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAT5044300.1"
                     /translation="MSIQQVCLVIVLTPLVGSAIAGFFRNQIGRVGAHTVTILGVAIS
                     LACSIFLAWGLFSGSIPNTNSLVYHWASGGALIPYEFNIGFLIDPLSVVMLVIVNFVS
                     LLVHVYSIGYMADDDGYQRFFSYISLFTFMMLMLVSANNFLQLFFGWEGVGLVSYLLI
                     GFWYQKESAIEGSLKAFLVNRVGDFGFVLGIGLIFAYSGSLDYEKVFTSANYLASQNI
                     ELFSGYSWSLITVICLLLFVGAMGKSAQVPLHVWLPESMEGPTPISALIHAATMVTAG
                     VFMIARISPLIELSTAALSTVLVIGATGALFTGILALVMNDIKRVVAYSTLSQLGYMM
                     VAMGASAYSAGMFHLLTHACFKALLFLGAGSVIIGMHHEQDMRKMGGLWNKMPITYVT
                     YVIGSLALCAFPPFAGFYSKDTIIEAAQLSQIPGSSYAYFCVTAGAMVTALYTFRSLF
                     MTFHGKPRMDEHTLSHLHESPWVVWLPLVLLAIPSIILGYVLYMPILFDTPSLLGSSI
                     FVLPEHNVLAELAHEVSSPFASAVHAIYSLPFWITVLGAVIAWICYIAVPSIPGYLAR
                     YFSIIYSILLNKYGFDRFNELFFVKGARGLGIAFYKIGDQKLIDGAVVNGSGRLVRWF
                     SGKGRKIQSGYIYHYATVMVFGLLAFLCWLILD"
     gene            complement(6314..6619)
                     /gene="nuoK"
                     /locus_tag="I9760_002552"
     CDS             complement(6314..6619)
                     /gene="nuoK"
                     /locus_tag="I9760_002552"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAT5044301.1"
                     /translation="MIPVYDYLVLGVILFGLSLVGIMLNRKNIILLLVCVELMLLAVN
                     TNFIAFSHYYNEVGGQVFVFFILTVAAAEAAIGLAIVMLLYRNRGNIDVDKMNHLKG"
     gene            complement(6637..>7012)
                     /locus_tag="I9760_002553"
     CDS             complement(6637..>7012)
                     /locus_tag="I9760_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAT5044302.1"
                     /translation="FGLIIVALLTGLLMVAIPKDLFKNSVQTQEKPLSVNSQLMDLST
                     EDGLVTPEKATSNTEKLGMVLYTDYILAFEIAAVILLVAIVSAITLVHRGAIRSKRQD
                     ITQQIMTRRAERVKLISMKSEK"
BASE COUNT         2419 a         1507 c         1189 g         1897 t
ORIGIN      
        1 cgagttctgc ttacgaacaa ttgtggtcct ctcgcctgcg gcgtgatttc gtacagatat
       61 gctcgaactc tgtcattggg acgaaacatt tcatgaggca acatttcaga gcgtggcaag
      121 aaagcctctg ctttaccacc taaatcaata attatattgt cacgagttac ttttttaaca
      181 gttccataga ttagctgtcc aagtttgcta cggaattggt ctatgatcaa ttcgcgttcc
      241 gcttccctga ctttttgcat aataacttgt cgcgctgttt gcgcttcaat tcttccaaat
      301 tctatggaag gaataatttc ttcaattcgg tcaccaacag cagccgaggg ctttcgctcc
      361 tttgcttgtt ccaaggacag ttggcggtcc ggaaactcta attcttcttc attcactaca
      421 tcccagtatc taaaggtttc atagtcaccg gttttagaat ctattttgat acgtacacct
      481 atatctaaac caagaatctt tcgagttgca gattccagtg cagcctgaat ggcaagtata
      541 atgacgtctt tactaacgcc tctttcgttt gataatgcct cagcaactaa taacaattct
      601 ttgctcattg ttcgcctctc tagactgtca aatttgcttt cacaatattt gataaaagta
      661 tttcttgatg ttcattatta atatccaata ctattgtatc ttctgatgct gaaacaataa
      721 tacctgataa ttttcgcttc ccattgactg gcttaaaagt ttttacatga atttcataac
      781 caaggtaacg ctgatattgc cagatactaa acaaaggccg gggtatccca ggagatgata
      841 tttccaaact ataatgccct ggaatagggt cttcaacatc aagcattgca ctgacttgct
      901 tactcacttc ctgacagtca tcgattccta ttccatctgg cttgtctata taaattctta
      961 ataacgagtg tttcccttgg gaaagatatt cacagcccca aagttcataa cccatatttt
     1021 tgattatagg ctctaatagc actatcaagt catcatttat catagtatgt ataaacaaaa
     1081 aaagggcgca ctcgcccaag cctggtaact gacttagatt tagacagtat cctagtcaac
     1141 ataataaaaa accccaaaaa tggggtttta taaaattggt agcgggggca ggatttgaac
     1201 ctacgacctt cgggttatga gcccgacgag ctaccaggct gctccacccc gcgtcaactg
     1261 agggcaagta tactgatact tgggtcccta agcaagtact tttgttaaag tttattaatt
     1321 aatttgcaaa tgcattaata caggctgcaa ttaaagcgcc tgggaaaata cctaaatata
     1381 gtaaggagag acaattaagt gaataaacta tggtattgcc tgtaccaatt cttactatct
     1441 cttcattttc aggttgatca aaatacatga ttttaacaat tcgcaaataa taataggcac
     1501 ctataaccgt aaatagcaaa cctacaacag ctaaccaagt catatgaaca tctactaatg
     1561 ccttcaatac caataatttt gtaaagaagc caaccgttgg tggcacgcca gccattgaga
     1621 acatgactaa tagcatcata aacgccagcc acgggtttct tttattcaag ccttttaagt
     1681 catcaatact atcaatttcc attccatgat ttgacatcaa tacaattaaa ccaaaagcag
     1741 ccgctgacat tacagcatag actaaaatat aatacagtgc tgcggcataa ccagcagagc
     1801 tggcggctaa aactccaaat aaggcatatc ctatatgaga aattgcagaa taagctaaca
     1861 agcgtttgat tctggtttga atcactgcta acaaattacc tatgccggta gaaaggagag
     1921 ccattactaa aataatttgc tgccattgca tggtgatgtc gactaagcca atagttaaaa
     1981 atcgcagtgc catccccagt gcagcaatct tgggtgctgt actaataaaa attgttacag
     2041 agcttggtgc cccttgatat acgtctggag cccacatatg gaaagggaca gcagccagtt
     2101 taaatcctac accggcaagt ataaatacca ttgcaaacgc aaaaagagta ttttgttgct
     2161 gccaattgac tgccactgca ttagcaacat ctaacaaatc aagttttcct gttgcaccat
     2221 acaccaggga tattccatac aacagcatac cagaagcaat agcacccata acaaaatatt
     2281 tcattgccgc ttcggatgca tcactatcag ttcggcggat ggcggtcatt gcatagagcg
     2341 gcaatgacat taactccaaa cctagataaa gagacaacag cgagtgtgct gaaacaagaa
     2401 tcatcatacc tagtgttgaa aacagcccca gaacatagta atcacctgta ggcatttgcc
     2461 gttcatccag atagttttgg gaatagacaa aacttaataa aaccgtgata tcaataaaca
     2521 gtttcattaa atgacttata tcatcactta taaataattt gttgaatata agtaacttgt
     2581 agcttcctat aaatacaaag cttactgctg cagaaactat aattcctaca caagatatgt
     2641 aaaatgcaat tgattttaat ttatgacgaa agaacaaatc agaaagcaac gccaaacacg
     2701 cggttattaa aataatcatt tctggtattg ctacatggat attttctagt aatgtcatca
     2761 tatttaacct tgctaaggaa cttgattgca atgtaagcct atcgcagttc tttgttagag
     2821 tttagattta tcagccagtg ccaaagtatg acttacagtt tgatgcacaa actctaacat
     2881 gggtttggga tacactccta gagcaatgac cattattgca agcaatacat aagcacttaa
     2941 ttcaaaacct gatatatctt ttaaggcagc aaccttttta ttagctacag gaccaaaaat
     3001 aactcttttg tacatccata gcgtatatgc tgctccaata atcaatgtag ttgcagccca
     3061 aaatgcaacc caaaaaccag cctttatact acctaatatc accataaact caccaacaaa
     3121 tccggatgtt cctggcaagc cggcattcgc catagcaaaa agcataaaaa aagaagcaaa
     3181 tataggcata gtatttacta ttccgccaaa atccgcgagc tgccttgtat gcatacgatc
     3241 atatataaaa cctacacctg caaacattgc acttgagaca aaggcatgcg atatcataac
     3301 gacaattgcg ccctcaagga ttaaaccggc gttgcttagt cccccatgac gagcaattgc
     3361 aaatatagcg aaacagccta gagttacaaa acccatatgt gaaattgaag aatatgcaat
     3421 caattttttc atatccttct gaataattgc aatgagtcct atataaacca ctgcgatcaa
     3481 agataaggca accatcagag gagcatattt gctgcaagcg tccgggacta ttggtaaaga
     3541 aaaacggata aatccataag cacccagttt tagcaaaatt gctgctaaaa cgacagaacc
     3601 accagcggga gcttcagtat gcgcatctgg caaccaggta tggactggaa acatcggtat
     3661 ttttattgca aaacctaaga aaaatgcaat aaaaatcaat gtttgtgcag tcatgcctaa
     3721 tttgatagca taaaaggttt caatcttaaa tgaacctgca atgtagccca tgtacaggaa
     3781 actggccagc atgagaactg accctaaaaa agtatatagg aaaaatttta ttgctgcata
     3841 aactctgtta tcagaacccc aaatacctat aatcagatac attggaatca aagttgcttc
     3901 ccagaaaata taaaatacta tggcatccat agcagaaaat ataccgacca gaaggccttg
     3961 cataattaaa aaggctgata aatattggga aactttttta ctaatgctat cccatgtagc
     4021 caaaatcact atcaaattag taaaaatgga taaaacaatt aatagtaaag ataacccatc
     4081 aataccaaga gtataattaa tacccagtgc cggcatccag gggatttcct ctataaattg
     4141 catgccaaaa gattgcagat caaatccttt aactaaaggt atgcagagca ctaaacagag
     4201 aagtatggtg aataatgtta aataacgcga tacatttgga tttttatcat ccccagtcag
     4261 caaaacaaaa acgccaccca atataggtaa ccaaattaat aaattgagca aataatgcat
     4321 accttcacct tatcttcagt ccaggattaa ccagcataaa aaggccagca atccaaacac
     4381 cattactgtc gcataatgat agatgtaccc actttgtatc tttcgccctt tcccagagaa
     4441 ccagcgaacc aggcgtccac taccattgac aactgcacca tcaattagtt tttgatcacc
     4501 aatcttgtaa aaagcaatgc ctaatcctct agctcctttc acaaagaaca actcgttaaa
     4561 gcgatcaaac ccgtatttat tgagtaaaat agaatagata attgaaaaat acctagctaa
     4621 ataaccagga atagatggga cagcgatata acaaatccag gcaataactg ctccgagtac
     4681 tgtaatccaa aatggcaaag aataaatcgc gtgaacagca cttgcaaatg gcgaacttac
     4741 ttcatgagca agttcagcta aaacattatg ctcaggtaaa acaaaaattg atgagcctaa
     4801 gaggcttggt gtatcaaata gaataggcat gtaaagcaca tagcctaaaa ttattgatgg
     4861 gatagcgagc aatactaatg gtaaccatac tacccatgga gattcatgaa gatgacttaa
     4921 tgtatgttca tccatacgtg gcttaccatg aaatgtcatg aataaggatc tgaaagtata
     4981 taatgctgta accatagcgc ctgctgttac acaaaaataa gcataactgc tgccgggtat
     5041 ctgggaaagc tgcgctgctt caataatggt atcttttgaa taaaaaccag caaaaggagg
     5101 aaatgcacac aatgccaaac tgcctatgac ataggttaca taggtaatcg gcattttatt
     5161 ccataaccca cccattttcc gcatatcttg ttcatgatgc atgccaataa tgactgaacc
     5221 agcaccaagg aacaatagcg ctttaaagca agcatgcgtc aataaatgaa acataccggc
     5281 actataggct gaggcaccca ttgccaccat catatagcct agttgtgata aagtagagta
     5341 tgctaccacg cgctttatat cgttcataac caaagccagt attcctgtaa acaatgctcc
     5401 tgtagcacca attactaata ctgtgctaag cgcagctgta gacaattcaa ttaaaggtga
     5461 gatgcgagca atcatgaaaa cacccgctgt caccattgtc gccgcatgga ttagcgctga
     5521 aataggggtt ggaccctcca ttgactcagg taaccaaacg tgaagtggca cctgtgctga
     5581 tttgcccatg gcgccaacaa aaagcagcag gcaaataact gtaatcaggg accaggagta
     5641 gccgctaaat aactctatgt tctggcttgc taaataattt gcgctggtaa atactttttc
     5701 ataatcaagg cttccagaat aagcaaaaat taaacctatt cctaatacaa atccaaaatc
     5761 tcctacacgg tttacaagaa atgctttcag gctgccttct attgcagatt ccttttgata
     5821 ccaaaatccg attaataaat aagaaacaag cccaacccct tcccagccaa aaaataattg
     5881 cagaaagttg tttgcgctaa ctaacatgag catcatgaat gtaaataaag agatataact
     5941 aaaaaatcgc tgataaccat catcatctgc catgtaccca atactgtaga catggactaa
     6001 caaagaaaca aaattaacta taaccagcat cacaacgctt agagggtcta ttaaaaaacc
     6061 aatattaaat tcgtagggaa ttaacgcccc cccgctagcc cagtggtaaa caagactatt
     6121 agtgtttggg attgaaccag aaaacaggcc ccacgcaaga aaaattgaac aggctaagga
     6181 aatagctacg cctaaaatag ttacagtatg agctccgact cgtccaatct ggtttctaaa
     6241 aaatcctgct attgcagaac caacaagggg agtcaaaacg attactaagc agacttgttg
     6301 aatactcacg attttaacct tttaaatgat tcattttatc aacgtcaata ttgcctctat
     6361 ttctatagag caacatgact atggccaaac ctattgctgc ttccgctgca gctacagtta
     6421 aaatgaaaaa gacaaatacc tgtccaccta cttcattgta gtaatgagaa aatgcaataa
     6481 aattggtatt cacggccaag agcatcaact caacacaaac aagtaataag atgatatttt
     6541 tacgattgag cataatacca acgagactca agccgaacaa aatcactcct aatactaaat
     6601 aatcataaac aggtatcata aagaattccc caatagctat ttctctgact tcatactgat
     6661 caacttcact cgttctgccc ttcgagtcat aatttgctga gtgatatctt gtcgttttga
     6721 cctgatagcc cccctatgca ccaaagtaat tgcagacaca atagccacta gtaaaatgac
     6781 ggcagctatt tcaaaggcaa gaatataatc tgtataaagg accattccta acttttctgt
     6841 gttcgatgta gccttttcag gtgtaaccag cccatcttcg gtagataaat ccattaattg
     6901 tgagttaaca ctcaaaggct tttcttgagt ttgcacactg ttcttaaata aatctttagg
     6961 gatagctacc attaataacc cagtcaataa ggcaactatt attaatccaa at
//
LOCUS       DACTJE010000166         6989 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.167, whole genome shotgun sequence.
ACCESSION   DACTJE010000166 DACTJE010000000
VERSION     DACTJE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.167"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..587
                     /locus_tag="I9760_002554"
     CDS             <1..587
                     /locus_tag="I9760_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAT5044303.1"
                     /translation="ITGKPIKFIGSGEKIEALEPFHPERIASRILGMGDILTLIEEVE
                     RKADKQASEKLAKKLKKGKGFDLEDFKQQLLQMNNMGGISGMMSKLPGVGQLPKQAMQ
                     QVNDKAMAQTIAIINSMTPKERRIPKIIVGSRKKRIALGSGTQIQDVNRLLKQFEQMQ
                     KMMKKFTKPGGMQKMMRGIGGMAGLKGMFPDDLK"
     gene            674..934
                     /gene="rpsP"
                     /locus_tag="I9760_002555"
     CDS             674..934
                     /gene="rpsP"
                     /locus_tag="I9760_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAT5044304.1"
                     /translation="MVVIRLSRAGAKKRPFYHMVVTDSRKRRDGNYIERIGYFNPVAR
                     GQEVKLHIDMDKMTHWQKVGAQLSDRVSALLKEHSKKSETAA"
     gene            940..1449
                     /gene="rimM"
                     /locus_tag="I9760_002556"
     CDS             940..1449
                     /gene="rimM"
                     /locus_tag="I9760_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAT5044305.1"
                     /translation="MNNKTNWVIIGRFGRPHGIKGFVTVHSFTDPADNILRYNDWHVF
                     LNKQWQPLKLLTIEVRSKAIIAQIEGYPKRELVSALTNLDIGVQESQLAALAPGEYYW
                     YQLIGMSVINSKGDLFGKVVEIMPTGSNDVLVVEGEKRHLIPYLPGQFVINVDESQQV
                     ITVDWDMNF"
     gene            1449..2201
                     /gene="trmD"
                     /locus_tag="I9760_002557"
     CDS             1449..2201
                     /gene="trmD"
                     /locus_tag="I9760_002557"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAT5044306.1"
                     /translation="MALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSS
                     RPYKQVDDKPYGGGPGMVMMYEPLHAAIKHARSEMKENCKTIYLSPQGKVVRQNDLKQ
                     IAAQKQSLLFVAGRYEGIDERIISHHVDEEWSLGDFVLSGGELAAMVFIDAIIRLIPG
                     SLGHLGSAEQDSFMNGLLDCPHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKT
                     WLKRPDLLEKVQLSETDKQLLAEFKCEHGDSC"
     gene            2228..2593
                     /gene="rplS"
                     /locus_tag="I9760_002558"
     CDS             2228..2593
                     /gene="rplS"
                     /locus_tag="I9760_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAT5044307.1"
                     /translation="MTNIIDQINAEQMQGKEIPDFNPGDTVLVQVKVIEGNRERLQAF
                     EGVVIAKRNRGLNSAFTVRKISHNVGVERVFQTYSPIVDSITVKRRGDVRRAKLYYLR
                     NLAGRAARIKEKLSGKKGD"
     gene            2609..3064
                     /locus_tag="I9760_002559"
     CDS             2609..3064
                     /locus_tag="I9760_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAT5044308.1"
                     /translation="MLLMSIFNTPVGNLEVAHDEHFIFNASFTDKSGAPAQNQLAHLI
                     ASELKAYFDNPHHRFQLPLKPQGSLFQQKVWNTLLVIPVGRTVTYGELATKLQSSPRA
                     VGQACKCNPLALFVPCHRVVGKHDLGGYMGNPNAISYKKNLLAHEAKII"
     gene            complement(3165..4028)
                     /locus_tag="I9760_002560"
     CDS             complement(3165..4028)
                     /locus_tag="I9760_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044309.1"
                     /translation="MLSSTKEYLQALRDGKYLLFLQWPKFIAEYYGKSDKNQEADEMV
                     SLLIFEWLNNGFCLDDIKKFAILYAVHEMESRPLREGLSYALTTISIALFPCMVYLTN
                     NLQEHYITSKKLSSKEVLQLMTMNNAYLEKQRFVEFLGQEQDKFFTWVKEADSSAVSK
                     AFDQIYSVTYLKYLIEDYLSLLESAHLPTDQLKSSRISLVVRLAKYLHEQTELTQDVH
                     DEIAVYVKKLWEMQPAEFEEEFLKKISPLPFIDNTVRILTGLSARFFSILQPPSPPLN
                     INSDTDKNPKV"
     gene            4177..4458
                     /locus_tag="I9760_002561"
     CDS             4177..4458
                     /locus_tag="I9760_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044310.1"
                     /translation="MKNKLAVSILLFLLPTAAAMANCDLTKFRWDCDLPVQVKPSPGA
                     SSLVYCGSSYGYITKQQYDILARYQRASVNMVLDINGEYIDSPCIGAER"
     gene            4643..5350
                     /locus_tag="I9760_002562"
     CDS             4643..5350
                     /locus_tag="I9760_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAT5044311.1"
                     /translation="MHIQIDGIPIEILRKPIKNLNLRIYPPDGLVKVSVPLKYSEQLI
                     KQQLQEKSKWIRAQCEQIRARSFYREPSLQTGATIPFKGKSYLLIVEEHYGPSQILIK
                     NELIYCYTKPDLTPQQKQNILDRWYRKEMNLVIPRLIQQWEAIMKVKINQWGIKKMKT
                     RWGSCNPRAQRIWLNLNLIKKPLECLEYVLVHEMVHILEASHNKRFYALMTQFMPKWQ
                     EYQRILEGTPNASTFAR"
     gene            5436..6074
                     /locus_tag="I9760_002563"
     CDS             5436..6074
                     /locus_tag="I9760_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HAT5044312.1"
                     /translation="MFNPDLIAHNICTQGFHIIDEFLEEAHYCSLRNMAYELKQQNLF
                     RNAKIGQNLQAQQNDKIRSDQIFWIDADSPYPPIQAYLKKLISLGNILNQTMYLGLAE
                     FETHFAIYLPGTFYRKHIDQFESTKTRKISFVYYLNDTWQKEFGGELLLYNIEDELIQ
                     NISPLGNRFVCFDSQLPHEVYPTQRTRFSMTGWMKTRSTSVLADNKLTKITQ"
     gene            6161..>6989
                     /gene="vipA"
                     /locus_tag="I9760_002564"
     CDS             6161..>6989
                     /gene="vipA"
                     /locus_tag="I9760_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VipA"
                     /protein_id="HAT5044313.1"
                     /translation="MKHYDSMDQELITMPISNAFLKLFSEFVDNYARNGTPSFYHWAK
                     EQNHEVINKFFHELLEIIKSNTETFHDVSIIEHLNATVLLSAKTNPYLLDCWISFITK
                     YNAETNSFTPIGASMHIKVGPEGTPSERIIRELKESLVMTQQAFILAQKANATEVDSL
                     KRQLEQTKQENERLVTQNQFLKDQVRAEKTFADLNPQINNARQQLSALTELIQTLSEI
                     TNAKPKIETVSSLSIPVVTENKSELLKENSCSVASVSKEVTPAISQEPKKRPVPPPPN
                     "
BASE COUNT         2361 a         1334 c         1338 g         1956 t
ORIGIN      
        1 aaatcacagg gaaacctata aaatttatag ggagtggtga aaaaatagaa gccttggaac
       61 cctttcaccc tgaacggatt gcatcaagaa tattggggat gggagatatt ttaactctca
      121 ttgaagaggt agagcgcaaa gccgacaaac aagccagcga aaaactggcc aaaaaattaa
      181 aaaaagggaa agggtttgac ttggaggatt tcaaacaaca attattgcaa atgaacaata
      241 tggggggtat ctccgggatg atgagcaaat tacccggagt ggggcaattg ccaaagcaag
      301 ccatgcagca agtgaatgac aaggcgatgg ctcaaaccat tgcaatcatt aactctatga
      361 ctcccaaaga acgccgtata cccaaaatta ttgtaggctc acgtaaaaaa cgcatagctt
      421 taggctctgg aactcaaatt caggatgtca acagactatt aaaacaattt gaacagatgc
      481 aaaagatgat gaaaaaattt accaaaccag gcggaatgca gaaaatgatg agaggaattg
      541 gtggcatggc cggattgaaa ggcatgtttc cagatgattt aaaataattc gtaagaatta
      601 cttcacatgt cgatgtaatt ttcgtacaat acacggcatt tttatgtaac cactctaaca
      661 gtagaggaaa ataatggtcg ttatacgttt atcacgagct ggcgctaaaa agcgtccttt
      721 ttatcacatg gttgttactg acagccgcaa acgtcgcgat ggaaactaca ttgagcgtat
      781 tggttatttt aaccctgtag cacgtggaca agaagtaaag cttcacattg atatggataa
      841 aatgacccac tggcaaaaag ttggggccca gttatctgat cgagtcagtg ccttgttaaa
      901 agaacatagc aaaaaatcag agacagctgc ttaaccaaag tgaataacaa gacaaattgg
      961 gttatcatcg gccgttttgg ccgaccccat ggtattaaag gctttgtcac ggtacattcc
     1021 ttcacagacc ctgctgataa tatcttgcgg tacaatgact ggcacgtttt tttaaataaa
     1081 cagtggcagc cccttaagtt attgacaata gaagtaagaa gtaaagcgat tattgctcaa
     1141 attgaagggt acccgaagcg tgaattagtc tctgcattaa ccaatttaga tattggtgtg
     1201 caagaatcac agcttgcagc tttggcacca ggtgagtatt actggtatca actgattggc
     1261 atgagtgtca taaactccaa aggcgatctt tttggaaaag ttgttgaaat catgcctact
     1321 ggctcgaatg atgtacttgt tgtggaaggt gaaaaaagac atttaattcc ctatcttcca
     1381 ggacaatttg taatcaatgt tgatgaaagc cagcaagtaa tcactgttga ttgggatatg
     1441 aatttttagt ggcattacat cttggagtca tcaccctgtt gcctgaaata atacaaggca
     1501 tccattatgg ggtaaccggc cgagctattg aacaaggctt ggttaaaatt gattgctgga
     1561 atcctagaga ttggtcatca agaccttata agcaagtgga tgacaagccc tatggtggtg
     1621 gaccaggaat ggtcatgatg tatgagccat tgcatgctgc aataaagcat gcgcgcagtg
     1681 aaatgaagga aaactgcaaa acaatttact taagccctca aggcaaggta gttagacaga
     1741 acgatttaaa acaaatagca gcccaaaaac aatccttgct atttgttgca gggagatatg
     1801 aaggaattga tgagcgaatc attagtcacc acgtagatga agaatggtcg ctaggggatt
     1861 ttgtattaag tggcggtgaa ttggccgcaa tggtatttat tgatgcgata attcgcttga
     1921 tacctggaag ccttgggcat ttgggttcag cagaacaaga ttcatttatg aatggattac
     1981 tggactgccc acattatact cggccagcaa ctataaatgg actggatgta cctgatgttt
     2041 tattaggagg aaaccataaa gaaatagaac gctggcgtag gaaacaatcc ctgggtaaaa
     2101 cctggctcaa acgcccggat ttacttgaga aagtacaatt aagtgaaaca gacaagcaat
     2161 tgcttgctga gtttaaatgc gaacacggcg attcctgctg aggatgagcc aatttgagga
     2221 gtagtccatg actaatatta ttgaccaaat taatgccgaa caaatgcaag gcaaagaaat
     2281 tcctgatttc aacccaggcg atactgttct ggtccaagta aaagttattg aaggtaaccg
     2341 tgagcgttta caggcatttg aaggcgttgt gattgccaag cgtaaccgtg gacttaactc
     2401 cgctttcact gtacgtaaaa tttcccataa cgtcggtgta gagcgcgttt tccaaaccta
     2461 cagtcctatt gttgatagta ttacggtgaa aagacgtggt gacgttcgtc gtgccaaatt
     2521 atattactta agaaatcttg ccggtcgcgc tgctcgtatt aaagaaaaat tatctgggaa
     2581 gaaaggcgat taattcttga atggtaaaat gctcctcatg tctattttta atacacctgt
     2641 gggtaacttg gaagtagccc atgatgagca ttttattttc aatgcgtcct tcacagacaa
     2701 atctggcgct ccagcccaaa accaattggc ccatttaatt gccagtgaat tgaaggctta
     2761 cttcgacaat ccccatcacc gttttcagct ccctctgaaa cctcagggaa gcctcttcca
     2821 gcaaaaggtt tggaatacgt tgttagtcat tcccgttggc agaacagtta cctatggaga
     2881 actcgctaca aaactgcaaa gtagtccaag agcagttgga caagcctgta aatgcaaccc
     2941 actggcttta tttgttcctt gtcatagagt agtagggaag catgatcttg gaggatacat
     3001 gggaaatcca aacgccattt cttataaaaa aaatctgctc gctcatgaag caaaaatcat
     3061 ctgatgaatt aagtttataa aaatcgatat tacatagatc agaacttaca aaagcaccaa
     3121 ggcaaaaaat attcttaacg aagagattag caagtcgtgt gagattaaac tttgggattc
     3181 ttatccgtat cagaattgat attcaaaggt ggggaaggag gttgtaatat actgaaaaat
     3241 ctggcgctta atcccgttaa tattcgtaca gtattatcga taaacggcag cggcgaaatt
     3301 ttttttaaaa attcctcttc gaactcagca ggctgcattt cccatagctt ttttacatac
     3361 acggctattt catcatggac atcttgagtt aattcggttt gctcatggag atacttggct
     3421 aatcgaacaa ccaagctgat gcgagaagat tttaattgat cagttggcaa gtgtgcagac
     3481 tctaataaac taagataatc ttcaattaaa tatttcaaat aggttacaga ataaatttga
     3541 tcaaacgctt tagaaaccgc tgaagagtct gcttccttaa cccaagtaaa aaatttatct
     3601 tgctcttgcc ctaaaaattc gacaaaccgt tgcttctcaa gataagcatt gttcatagtc
     3661 attaattgga gcacttcctt acttgataat ttttttgaag taatataatg ctcttgcaga
     3721 ttatttgtta aatacaccat acagggaaaa agagcgatag aaattgtagt gagtgcatag
     3781 gataatccct ctcttaaagg cctggactcc atttcatgaa cggcatacaa aatagcaaat
     3841 tttttaatat catccaaaca aaagccatta tttaaccatt caaatatgag caagctcacc
     3901 atttcatcag cttcttgatt tttgtcgctt ttaccataat attctgcaat aaattttggc
     3961 cactgcagaa atagaaggta ttttccatca cgtaaagctt gaagatattc ctttgttgat
     4021 gaaagcatag ttatccatta ccaaaatctg attattcaat ttataacatt aacaatacta
     4081 aaaatcttgt gaaaaattat ttcatgttaa acaacatacg cacatcaatt gttatcctat
     4141 acttaaagta aaacgacaaa tcaggcgggg gattccatga aaaataaatt agcagtgagt
     4201 atattattat tcctgttacc aacagctgca gccatggcta attgtgattt aaccaagttt
     4261 cgatgggatt gtgatcttcc tgtacaggtt aagcccagtc caggcgcttc ctctctagtg
     4321 tattgtggaa gctcttacgg ctatatcaca aagcagcaat atgatattct ggctcgctat
     4381 caacgcgcca gtgtcaatat ggttttggat atcaatggtg aatacattga tagcccgtgt
     4441 ataggagccg agcgttaaaa ttattgcagc aatgacttaa aataaacaat gagaaaaaga
     4501 aaaggaatat tattatttcc agcaaatttc ttattcaaaa tcagaagttt ctgcttgtgg
     4561 ctattcaatg cactcaacta ttaatatcat aaatattact tcttctgtat aatctaaaac
     4621 aaatcaataa gcatattttg ctatgcacat tcaaattgat ggcattccta tagaaatact
     4681 gagaaaaccc attaaaaatt taaatttgag aatttatcca cccgatgggt tagtgaaagt
     4741 gagcgtccct ttaaaataca gcgaacagct aataaaacaa cagctgcagg aaaaaagtaa
     4801 atggattcgt gcccaatgcg agcaaattcg cgcacgatct ttttatcgtg aaccatcgct
     4861 tcaaacaggt gcaaccatcc cattcaaagg taaaagttat ttattaattg ttgaagaaca
     4921 ttatggccct tctcaaatcc tgatcaaaaa tgaattaatt tattgctata caaaacccga
     4981 tttaactcca cagcaaaagc agaacattct ggatcgctgg tatagaaaag aaatgaatct
     5041 tgtcattccc aggcttattc aacaatggga agccatcatg aaagtcaaaa ttaatcaatg
     5101 gggtataaaa aaaatgaaaa cccgctgggg ttcgtgtaat cctcgcgcac aaagaatctg
     5161 gctcaatctt aatttaatca aaaaaccttt ggagtgcctt gaatacgttc ttgttcatga
     5221 aatggtgcat attttggaag ccagtcataa caaacgattt tatgcactca tgacacaatt
     5281 catgcccaaa tggcaagaat atcaaaggat actggaaggc acacctaatg ccagtacatt
     5341 tgcaaggtaa tcatcgaatt cattacctca ttatttccaa aaaataattc ttggcgtaca
     5401 atgcattatc actttgcctg attggtgtag acaggttgtt taatccggac ttaatcgccc
     5461 ataatatttg tactcaaggc tttcatatca ttgatgaatt tctggaagag gcacactact
     5521 gctctttacg aaacatggct tatgaattaa aacagcaaaa tttattcaga aatgccaaaa
     5581 ttggccagaa cttacaagca cagcaaaatg acaagattag aagcgatcaa attttctgga
     5641 tagatgcaga ctccccttat ccgcccattc aagcttactt aaaaaaactt atctctttgg
     5701 gcaatattct gaatcaaacc atgtatcttg gtttagcaga atttgaaact cattttgcaa
     5761 tttatctccc tggaaccttt tatagaaagc acattgatca atttgaaagt acaaaaacca
     5821 ggaaaatatc atttgtctat tatttgaatg acacttggca aaaagaattt ggtggcgaat
     5881 tgttactcta caatattgaa gatgagctca ttcaaaacat ttctccattg gggaatcggt
     5941 tcgtctgttt tgatagtcaa ttaccgcacg aagtctaccc aactcagcga acgcgcttca
     6001 gtatgacagg ctggatgaaa acacgctcaa cctccgtact ggccgacaat aaactgacca
     6061 aaataaccca gtagcacttt atacgctgtt ctataaaaaa atgaataatt attttttaac
     6121 ttcacaacag ctattgaact ttaatttaag ttgatattaa atgaaacact atgattccat
     6181 ggatcaggag ttaattacca tgcctatcag taatgccttt cttaagttat tcagtgaatt
     6241 tgttgataat tatgcgcgca atggcacccc ttcattttat cattgggcaa aggaacaaaa
     6301 ccatgaagtc attaacaaat ttttccatga gcttttggaa attattaaat caaacacaga
     6361 aacatttcat gatgtcagta tcatagagca ccttaatgct acagtactat tatcagcaaa
     6421 gaccaatccc tacttactcg attgttggat ttcatttatt actaaataca atgctgaaac
     6481 caacagtttt accccaatag gggcttcgat gcacattaaa gtcgggccag aaggcacgcc
     6541 atccgaacgg ataataaggg agctcaaaga aagtctggtc atgacgcaac aagcgtttat
     6601 tttagctcaa aaggccaatg ctacggaagt tgattcttta aaaagacaac ttgagcaaac
     6661 aaagcaagaa aacgagcgct tagtcacaca aaatcagttt ctaaaggatc aagtcagggc
     6721 agagaaaaca tttgcagacc ttaacccaca gattaacaat gcccggcagc aactcagtgc
     6781 tttaacagaa ttaatacaaa ctctaagcga aatcacaaat gccaaaccca aaatagaaac
     6841 tgtatcttcc ttatccattc cagttgttac agaaaataaa tctgaacttc taaaggaaaa
     6901 ttcatgctct gtcgcttctg tatccaagga agttactcct gcaatcagtc aagagccgaa
     6961 aaaaagacca gttccacccc cccccaatg
//
LOCUS       DACTJE010000167         6726 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.168, whole genome shotgun sequence.
ACCESSION   DACTJE010000167 DACTJE010000000
VERSION     DACTJE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.168"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            148..1074
                     /gene="murB"
                     /locus_tag="I9760_002565"
     CDS             148..1074
                     /gene="murB"
                     /locus_tag="I9760_002565"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAT5044314.1"
                     /translation="MSITGMDSSHVTESQGTLLFNEPLAEYTTWRVGGPAARLYKPAN
                     IDDLALFLSRLPFDEPLLWLGLGSNSLIRDGGFSGTVILTQGCLKEMTLLSDNCIRVE
                     AGVSCASMARFSARNNLSGGEFWAGIPGTMGGALRMNAGCHGGETWQSVIEVQTINRR
                     GEIRTRKPEEFEVAYRHVAGLGDEWFISAKLQLSPGNKETSLQLIKDLLAHRAKTQPT
                     NEYNCGSVFRNPPGDFAARLIESCGLKGVSIGGAVVSEKHANFIINHQGTATAANIEA
                     LIHLVQTKVREQTSIELIREVHIIGDANVQTR"
     gene            1058..2152
                     /locus_tag="I9760_002566"
     CDS             1058..2152
                     /locus_tag="I9760_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAT5044315.1"
                     /translation="MSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAM
                     DKSGRFHRHDYNDLLACSDKLPVVTEKSTPLEGLLINGRLAVDAKIVFPVVHGPLYED
                     GCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQ
                     QFCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVG
                     REIELAVLENSIPCGKPRVSMVGEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSL
                     EEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEINTLPGFTSISMYPKLWQAT
                     GIAYPDLLDELINLAMVHHNCRQHLVTNYL"
     gene            2168..2887
                     /locus_tag="I9760_002567"
     CDS             2168..2887
                     /locus_tag="I9760_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAT5044316.1"
                     /translation="MEVNSVGLRYLTIMGLLILSALLLAGRLGYLYLADAERFPITTI
                     KVAATYEHITHKELENVLAKYLDASFFLLSVKGLQSELNSMSWIDTAYVERVWPDTLK
                     IKLTEKKPVAIWGDALMTRDGKLFNQGSVPSDLDIPKLKGPQSQQLEVLQVYEKLSKI
                     LSSYGLNASGLYLRDNQSWVLLLNHSVKIYLGKKELEERLLRFCKAYPAVFAEKADQL
                     AGVDLRYPRGMAVQWKQQMGR"
     gene            2887..4125
                     /gene="ftsA"
                     /locus_tag="I9760_002568"
     CDS             2887..4125
                     /gene="ftsA"
                     /locus_tag="I9760_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAT5044317.1"
                     /translation="MAKKIEKNIITGLDIGTSKIIALIGEVTSDGTIEIIGIGRHPSR
                     GLKRGVVVDIETTVNSIQRAVQEAELMAGCEVRTVYAGIAGSHIRSLNSHGIVAIRDQ
                     EVSQADVERVIDAAKAVAIPADQKILHILPQEFIIDHQGSIREPIGMAGVRLESRVHI
                     VTGSVSAAQNIVKCVRRCGLDVIDIILEQLASSHAVLTEDEKELGVCLIDIGGGTTDI
                     AIFCEGAIQHTSVIPIAGDQVTNDIAMALRTPTKAAEAIKVNHACALPELANPNQMLE
                     VTSVNDRPGRKISAKALADVVSARYEELFSLVRNELRRSGFEDRLAAGIVLTGGASNV
                     RGAIELAELCFEMPVRKGCAHYVSGLTEATENPSFATGVGLLLHGFQQQYESGYNVPA
                     MNDNGKSIWSRMKEWFQGNF"
     gene            4320..5516
                     /gene="ftsZ"
                     /locus_tag="I9760_002569"
     CDS             4320..5516
                     /gene="ftsZ"
                     /locus_tag="I9760_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAT5044318.1"
                     /translation="MFELMESSQQENNAVIKVVGVGGGGGNAVEHMVAENIDGVEFIC
                     ANTDAQALRGSSAKIHIQLGDALTKGLGAGANPQIGREAAEEDREHIKEILSGADMVF
                     ITAGMGGGTGTGAAPVFAEIAKELGILTVAVVTKPFSFEGKQRALAAEEGIRRLAEHV
                     DSLITIPNNKLLSVLGKNISLLNAFKAANNVLLGAVKGISDLITRPGLINVDFADVRT
                     VMSEMGMAMMGTGSAVGEQRARQAAEAAIASPLLEDVNFSGARGILVNITAGLDMSIG
                     EFEEVGDVVKEFISDDATVVVGTVIDPEMTDEMRVTVIVTGLGDNRQRQQQPQQPLRA
                     RLVETTRSDGSLDYQQLERPAVIRKQAQTGTATTATKQTNNGNESVPDVDYLDIPAFL
                     RRQEEA"
     gene            5764..6678
                     /locus_tag="I9760_002570"
     CDS             5764..6678
                     /locus_tag="I9760_002570"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAT5044319.1"
                     /translation="MIKQRTPKKVIQATGVGLHSGEKVLLTLRPAPVNTGIVFRRVDL
                     SPVVEIPASYEYVGDTMLCTTLHHGKVKIATVEHLLSALAGLGIDNAYIDVNAPEIPI
                     MDGSAAPFVFLIQSAGIREQNAAKRYIRILKPIRVEENGKYVQFLPHKGYKITFTIGF
                     EHPVFNDRPQTVSFDFSGTSYVKEVCRARTFGFLSDYEKLRECDLAKGGSLDNAIVVD
                     DYRVLNEDGLRFESEFVTHKVLDAIGDLYLLGSSLIGAFEGYKSGHELNNRLLRELMV
                     RQDAWEYTYFDTENYLPAVHPEYYPVEA"
BASE COUNT         2013 a         1107 c         1626 g         1980 t
ORIGIN      
        1 ggataaacga gtgaccatag tgagcgagca atcgttgaaa gccaccttag atgaatttat
       61 taaagatggt gacgttattt tgatgcaggg agctggaagt atcggccaaa tagcagtgaa
      121 cctgatgaaa aacatgtgag aatgaacatg agcataacgg gaatggacag cagtcatgta
      181 acagagtctc agggcacttt gcttttcaat gagcctttgg ctgagtatac tacctggcga
      241 gttggtggcc ctgcagcgag gctttataaa ccagcaaata tagatgattt ggctttgttt
      301 ttaagtcgat tgccttttga tgagccatta ttatggttag gtttaggctc taattctcta
      361 attcgcgatg gcggtttttc cggtaccgtg attctcactc agggctgttt aaaagaaatg
      421 accctattga gcgataactg tattcgggtt gaggcagggg tatcctgtgc cagtatggct
      481 cgtttttctg cgcgtaataa tctgtcagga ggagagtttt gggctggtat ccccggtacc
      541 atgggtggtg ctctgcgaat gaatgccggt tgtcatggag gtgaaacctg gcagtcggtt
      601 attgaagtac aaacgataaa tcgtcgaggt gaaatcagaa cccgtaaacc ggaagaattt
      661 gaagtggctt atcgacatgt tgctggcttg ggagatgaat ggtttatttc cgcaaagctt
      721 cagctatcac caggtaataa agagacatcc ttgcaattaa ttaaggattt attggctcat
      781 agagccaaga cccaaccaac taatgaatat aattgtgggt cggtatttag aaaccctcct
      841 ggagattttg cagcgcgact tattgaatct tgtggcctca agggggtaag cattggtggg
      901 gcagttgttt ctgaaaaaca tgccaatttt attatcaatc atcaaggtac tgccacggca
      961 gctaatattg aagccttgat tcatttggtg caaactaaag ttcgagagca aacatctata
     1021 gagttgatac gcgaagtaca tatcattgga gacgctaatg tccaaacccg ttaatttggt
     1081 tttactttat ggtggaaaat caggcgagca tgaagtgtca ttggtttctg ccgcatcggt
     1141 tctaaaacac ctggattccg agaaatatca tattataccc attgcaatgg ataaaagtgg
     1201 tcgatttcat cgtcatgatt ataatgattt gctcgcctgt tctgataaat tacctgttgt
     1261 tactgaaaaa tcaaccccat tggaaggctt attaattaat ggacgtctgg cagttgatgc
     1321 taaaattgtt tttccagtgg ttcatggccc tttatatgag gatggatgtt tacaaggttt
     1381 acttgagtta gccggtgtcg cctatgtagg atgtgacgta ttgtcttctg caatcggaat
     1441 ggacaaagac atggcgaggc gactcgcatg tattaatggg ctcaaatcag caagatataa
     1501 attattatca tggcatgcca atgcgtctga aagacaacaa ttttgtcatg aagtcgcctc
     1561 ggagtttggt tggcctctgt tcgttaagcc atgctcacta ggttccagtg tgggaattca
     1621 caaagcaaat aatatggatg agctaaacgc agcagttgct gatgcattac gctatgatga
     1681 agaaattctg gttgaagaat ttattgtcgg aagggaaatt gaactggcag tgttggagaa
     1741 cagtatcccc tgtgggaaac caagagtaag tatggtgggt gagataaaag taaaccaccc
     1801 tgacggttat tattcttata ctgcgaaata tttggaaagc agtcaaacgg atttgattat
     1861 tccagctcaa ttaaataata gtctggaaga acaattaaaa caggctgcag cgaatatttt
     1921 tagttatctt aaatgtaaag gcatggccag ggtagatttt tttgtaaatg ataagacaga
     1981 agaaatttac tttaatgaaa taaatacatt acctggattt acctccataa gtatgtaccc
     2041 caaattgtgg caggcaactg gtatagctta tcctgattta cttgatgaat tgattaatct
     2101 agcaatggtt catcataact gtcgtcaaca tttggtgaca aattacttgt gaagggaaca
     2161 ctaaattatg gaagtgaact ctgtcggctt gcgttattta acgataatgg gtttgttaat
     2221 tcttagtgct ttgctgctag cgggtcgctt aggctattta tatcttgctg atgcagagcg
     2281 ttttccaatt acaacgatca aagttgcagc gacgtatgaa catataacac ataaagaatt
     2341 ggagaatgtg ttagctaaat atctggatgc cagtttcttt ttattatcag taaaaggatt
     2401 gcaaagtgaa ttgaattcaa tgagttggat tgatactgcc tatgtagaaa gagtttggcc
     2461 agatacattg aaaattaaac tcactgaaaa aaagcctgta gcgatttggg gtgatgctct
     2521 aatgacaagg gatggaaagt tgttcaatca gggttctgtt ccatctgatt tggatattcc
     2581 caaattaaag ggtccgcaat ctcagcaatt agaagtctta caagtttatg aaaaattgag
     2641 taagatatta tcatcatacg gtttaaatgc ttcaggcttg tatttaagag ataatcaatc
     2701 ctgggtgttg ctgcttaatc atagtgtaaa aatatacttg ggaaagaaag agttagaaga
     2761 gcggttgctg cgtttttgta aagcataccc tgctgttttt gctgaaaaag cagatcagtt
     2821 ggctggcgtg gacctgcgtt atccacgtgg catggcagtg cagtggaaac aacaaatggg
     2881 acgataatgg ctaaaaaaat agaaaaaaat attatcacag ggttggatat tggaacttca
     2941 aaaattattg cattaattgg tgaagtaaca tcagatggaa cgatagaaat tataggtatt
     3001 ggccgtcatc cctctcgcgg attaaagcga ggagttgtag ttgatataga aaccacagtc
     3061 aattcaatcc aacgtgctgt acaagaagcc gaattaatgg ctggttgtga ggtgaggact
     3121 gtttacgcag gcattgctgg cagccatata cgtagcctta attcccatgg tattgtggcc
     3181 atccgtgatc aggaagtatc tcaagccgat gtggaaaggg ttatcgatgc agcaaaggcc
     3241 gtggcaatcc ctgctgatca aaaaatactt catatattac cgcaagaatt tatcattgat
     3301 catcaaggca gtattcgtga accgataggt atggctggtg taaggctgga atctcgggtt
     3361 catattgtta ctggttctgt cagtgctgcc caaaatattg tgaagtgtgt caggcgctgt
     3421 ggtttggatg tgattgatat tattctggag caattagctt caagccatgc tgttcttact
     3481 gaggatgaaa aagagcttgg ggtttgttta attgacatag gtggcggcac tactgacatt
     3541 gcgatatttt gtgagggagc tatccagcat acctcagtca taccgattgc aggcgatcaa
     3601 gtgacaaatg atattgcaat ggccttgcgt acgccaacca aggcggcaga agcaattaag
     3661 gtcaatcatg cttgtgcatt accagaatta gccaatccaa accaaatgtt agaagttacc
     3721 agtgtcaatg atagacctgg gcgaaaaatc tcggccaaag ctttggcaga tgtcgtttca
     3781 gctcgatatg aggaactatt ttcactggta cgtaatgaat tacgccgaag cgggtttgaa
     3841 gaccgtttgg ctgctggtat tgttctaaca ggtggagctt ccaatgtaag aggagcaatt
     3901 gagttggccg aactttgttt tgaaatgcca gtcagaaaag gatgtgcgca ttatgtctcc
     3961 ggtttgacag aagcgacaga aaatccttcg tttgcaacag gggttggttt actgcttcat
     4021 ggctttcaac agcaatatga aagtggctat aatgtgccag caatgaatga taatggtaaa
     4081 agcatttggt cacgcatgaa agaatggttt caaggaaatt tttagctttt catgtaaaaa
     4141 cactgatatg atgcttaaaa atgttcctgg tatggataga aattgagata tggggttgca
     4201 tgatattttg catgacaaca aatgcagatt tttgtcttgt aaagtgtcaa atgcgataag
     4261 ttcaaaactg tttaatttat ttaataagaa aaaagcagca cattagggga gacagggtta
     4321 tgtttgaatt aatggaaagc agccagcaag aaaataatgc tgtcatcaag gttgtaggtg
     4381 ttggtggtgg cggtggtaac gctgtcgaac atatggtcgc tgaaaatatt gatggtgtgg
     4441 aatttatttg tgcgaatacc gatgcacaag ctttaagagg ttccagtgcc aaaatacaca
     4501 ttcaattggg tgatgcattg accaagggct tgggggcagg tgctaatcca caaattggaa
     4561 gagaagcagc agaagaagac agagagcaca ttaaagagat tcttagtggt gcagatatgg
     4621 tctttattac agccggaatg ggcggaggaa cagggacagg agctgcgcct gtttttgctg
     4681 aaattgctaa ggaattaggt attttaacgg tcgctgtggt aactaaacca ttttcttttg
     4741 aaggaaaaca aagggcgctt gctgctgagg aaggtattcg acgcttggct gagcacgtgg
     4801 attcgttaat tactattcct aataataaat tattaagtgt tttaggaaaa aatattagtt
     4861 tgctcaatgc gttcaaagct gcaaataatg tgttgcttgg tgctgtgaaa ggtatttctg
     4921 atttaattac ccgccctggt ttgatcaacg ttgattttgc ggatgtgcgt acggtgatgt
     4981 cagaaatggg aatggccatg atgggaacag gaagcgcagt tggtgagcaa agggcacgtc
     5041 aagcggccga agcagcgatt gcatctcctt tattggagga tgtgaatttc tccggcgctc
     5101 gcggaatctt agtgaatatc accgcaggcc ttgatatgtc tattggtgaa tttgaagaag
     5161 tgggtgacgt ggttaaagaa ttcatttctg atgatgccac tgtagttgtt ggaactgtta
     5221 ttgatcctga aatgactgac gagatgagag taactgtgat tgtgactggt ttaggtgata
     5281 atagacagcg tcagcaacag ccacaacaac ctcttagagc acgcctggtt gaaacgacaa
     5341 gaagcgatgg gtctcttgat tatcaacaat tggagagacc ggcggtgatt cgtaagcagg
     5401 ctcagactgg tactgcaaca acagctacaa agcaaactaa taatggtaat gagagtgttc
     5461 ctgatgtgga ttacctggac ataccggcgt ttttacgtag gcaagaagaa gcatagaagc
     5521 atttttaacg ggatatcttg agagaggatg tgtttgaaaa caaggatgta tttgatggat
     5581 acaaacgttc tttcttgagt atctcatgaa ttgattcgtg tggttatttt ttgcaatttt
     5641 tgtatttggt ttccaaaacc gcattaattg aaattaaaaa aattagacta atcaataaat
     5701 tgttgctaag atcttaaggt ttttgcgtgc aaaataaata taagagagaa aggtgagcac
     5761 tgaatgataa aacaaagaac tcctaaaaag gtaatccagg ctactggtgt tggattacat
     5821 tccggtgaga aagtgctttt aaccttaaga ccagctcctg taaacacagg tatcgttttt
     5881 agacgcgtcg atttgtcacc agttgtcgaa atacctgcct cctacgaata cgtcggagat
     5941 accatgcttt gcaccaccct tcatcatggc aaggtgaaaa ttgcaacggt ggagcattta
     6001 ttatccgccc tggccgggtt aggaattgat aacgcctata tagatgtcaa tgccccagag
     6061 attccaatta tggatggaag tgccgcccca tttgtatttt taattcaatc agcgggtata
     6121 agagagcaaa atgcagcaaa aagatatatc cgaattctta aacctatacg agtcgaagaa
     6181 aatggaaagt atgttcagtt ccttcctcat aaaggatata aaataacatt taccataggt
     6241 tttgagcacc cggtttttaa tgatagacca caaacagtaa gctttgattt ttctggaaca
     6301 tcgtatgtca aagaagtttg tagggcgaga acttttggtt ttctttcaga ttatgaaaaa
     6361 ttgagagagt gcgacctggc aaaaggcggt agtctagata atgctattgt agtagatgat
     6421 tatcgtgtcc ttaatgaaga tggtctccgc ttcgaatcag aatttgttac tcataaagta
     6481 ttagatgcta tcggtgatct ttatttattg ggttccagtt taattggcgc atttgaagga
     6541 tataaatctg gtcacgaatt aaacaacaga ttgttgcgtg aattaatggt aaggcaggat
     6601 gcttgggaat atacctattt tgatacagaa aattatctgc ccgcggttca ccctgaatat
     6661 tatcctgttg aagcctgaca gaaatacatc atatccacgt agtgagaata ttcattttca
     6721 ctatta
//
LOCUS       DACTJE010000168         6673 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.169, whole genome shotgun sequence.
ACCESSION   DACTJE010000168 DACTJE010000000
VERSION     DACTJE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.169"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..419
                     /locus_tag="I9760_002571"
     CDS             <1..419
                     /locus_tag="I9760_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HAT5044320.1"
                     /translation="LDDYRLVKHNLQNPQKKLSSQNRLIPYTVFLDPELARIGLTEAQ
                     ARSQGRRIKIATIPAAAIPRAKTQGETTGILKAVIDAETDLILGVSIFCAEAGEILGV
                     IQLAMELRVPYQKLRDMMFAHPTLVEGINLWFAQII"
     gene            625..1224
                     /locus_tag="I9760_002572"
     CDS             625..1224
                     /locus_tag="I9760_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HAT5044321.1"
                     /translation="MKIVVIGATGIIGKAIVAELNPRHEIISASYKNSDIQVDITSKQ
                     SIDAMYKKLNTIDAIVLATGKVHFAALKDMGEDDYKIGLTNKLMGQVNVVLAGLEYIN
                     DNGSFTLTSGILNRDPILYGSSAAMVNGALDGFIKSAALEMPRGIRINGVSPTVITEA
                     MENYGPYFRGYEPVSAARAALAYSKSIEGAQTGQVYCVG"
     gene            complement(1437..2324)
                     /locus_tag="I9760_002573"
     CDS             complement(1437..2324)
                     /locus_tag="I9760_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAT5044322.1"
                     /translation="MKIAVVEPIGIAMQEIRSELPGHTIIECDSRQWSDEQLIDFVKD
                     ADIIALTNRRLSAAVINSALRLQLIAVAFAGIDHIDRDAVSKRNIPVKNAAGYANTAV
                     SELVFGLMISLARHIPDNNQRIREQGITNTGIELKNKVLGIVGYGAIGAEVARLAKAF
                     QMTTIIYDRKSQVTLDDLFSQSDFVTLHVPLTNETKGMVNLRLLSQMKKSAYLINCAR
                     GPIVVNSDLKKALEQDMIAGAALDVFDVEPPLPANYSLWEVPNLIATPHIGFNTREAL
                     VAKGQLTIRNIKEFLSSRL"
     gene            complement(2448..3380)
                     /gene="glsA"
                     /locus_tag="I9760_002574"
     CDS             complement(2448..3380)
                     /gene="glsA"
                     /locus_tag="I9760_002574"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAT5044323.1"
                     /translation="MSSKLLTIQLLEELVHAAELNQEGKTADYIPELANVNQELTAIA
                     VQALGEKTLAYSNNPLHPVTLQSTGKMIPLIGLLEEFGADQLFEWVKVEPSGDDFASI
                     TRLEQFGPKPSNPMLNAGAIALCSRIPGIGEQQFRWLEHWVQKLFNQRLSINPLVFAS
                     EKRTGNRNRALAYLLKSRNNLGADVHETLDLYFALCSYEAMLDQMLYLPTLLANRGKD
                     PDTGEQILSTETCKITLAIMATCGLYDETGTHMVKTGMPAKSGVSGYTIAVVPGKAGI
                     VVLSPRVNAKGNSIRGEIMLEGLSKAMNWHFALP"
     gene            complement(3640..4419)
                     /gene="ceg8"
                     /locus_tag="I9760_002575"
     CDS             complement(3640..4419)
                     /gene="ceg8"
                     /locus_tag="I9760_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg8"
                     /protein_id="HAT5044324.1"
                     /translation="MNDIMWYQNIMRNLLEKLPEASRKDGEFFLDKLFLAVKRVDNKP
                     EMEFFLGLIEKSFLKFENEKSGSVTLVKFSRYILGLTLLYIKSGNDGAIWNSDFIPYL
                     GEIKKFFDVEKEGKSFYKFLNQLEVDVFVSLNHQLAIDFKHFTSILRGNNDLKTIQSF
                     TEACKGLGNESSEGFNQVVKDAEAHMINLQFADDLNSDFNLEGIDENLRNTTLQKSSH
                     ANSMPMSVNKGHRLSIFKALATSGELSDQTSLFINQNNGPI"
     gene            complement(4503..5825)
                     /gene="gabT"
                     /locus_tag="I9760_002576"
     CDS             complement(4503..5825)
                     /gene="gabT"
                     /locus_tag="I9760_002576"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HAT5044325.1"
                     /translation="MKKIHIKTPIPGPKSQQLMELRRQHVARGPFHATPIFVKQAKGS
                     FVEDVDGNVFLDFSSGFGVVNTGHCPDSVVNAIKLQAEKFIHTGFNIIPYESYIKVCE
                     KLNDHTPGHFEKKSLLLNSGAEAVENAIKIARAYTGKQAVICFDHAFHGRTYMAMTLT
                     SKNKPYKHGFGPFPSEIHRAPFPYEYRWKGANCVEECFDEFTDLANFRVGVENIAAVI
                     IEPVLGEGGFIQSPALFLQKLREFCTTNDIVFIADEIQSGFGRTGNLFAMNTLGVPPD
                     LTISAKGLGGGVVLAGVTGKAEIMDAAMEGGLGGTFGGNPLSCAAALEVFHIFEEGSL
                     LQNVTHLAKALQSRLSGFKEKYKVVGDVRGLGVMQAIELVKDKNTKEPNKEATVQLAQ
                     FCLEHGLIILTCGTYGNVIRLHMPLSTGVKDLELGLSIIEEGLKKLSV"
     gene            complement(5830..>6673)
                     /locus_tag="I9760_002577"
     CDS             complement(5830..>6673)
                     /locus_tag="I9760_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAT5044326.1"
                     /translation="GVGEELASNAMVDKVAFTGGSATGRKIMQAATGNLKKISLELGG
                     KNPNIVFADSDLEMAIDGALFGAFANQGEVCSAGSRLLVERSIHKKIVEGMLKKIPNI
                     KLGHGLDAGVKMGPLVSSAHREKVESYVKLGMEEGAKLLCGGKRPTGNEFAKGHFFEP
                     TIFDEVKSTMRIAREEIFGPVLVVIPFDTEEEAIRIANDTDYGLSGAVWTKSVTRAHR
                     VTSQIRAGILWVNHYHPTYNEMPWGGYKQSGAGRELGLYGIESYLEVKQVNINLDETP
                     IGWY"
BASE COUNT         1999 a         1456 c         1235 g         1983 t
ORIGIN      
        1 ctcttgatga ctatcgtctg gtgaaacata atttacaaaa cccccagaaa aagctttctt
       61 cccaaaacag gcttattcct tacacagtgt ttctcgaccc ggaattagca cgcattggac
      121 ttactgaagc tcaagccaga tcccaaggcc gccgaattaa aatcgccaca attccggcag
      181 ctgcgattcc tcgcgccaaa acccaaggag aaacaacggg tattttaaaa gcagtcattg
      241 acgctgaaac cgatttgatt ttgggcgttt ctattttctg tgcggaggca ggggaaatat
      301 taggggtcat ccaattagca atggagttgc gcgtacctta tcaaaaattg cgcgatatga
      361 tgttcgctca ccctactcta gtggaaggca tcaatctgtg gtttgcgcaa ataatttgaa
      421 ttgatgtaaa accatactcg ccatattaca tgctacaagg caagtggtta atagaggcat
      481 tttacttctc ttcttcaata aatcctgtgt ataggcagta atcaaataca gccgatacac
      541 actttttatt tcagaactta ttgttgttac tgtactattc tatgtaggac ttttgctttt
      601 tattggataa agagggaatg acttatgaaa atagtggtga ttggtgctac ggggataatt
      661 ggaaaagcaa tagttgctga attaaaccca agacatgaaa ttatttcagc gagctataaa
      721 aacagtgata ttcaagtgga tataaccagt aaacaatcaa tcgatgcaat gtacaaaaaa
      781 ttaaatacta ttgatgcgat tgtgttagct accggtaaag tgcactttgc tgccctaaaa
      841 gacatgggag aagacgatta taaaattggt ttgaccaata aactaatggg tcaagttaat
      901 gtagtcttgg ctggattaga atacatcaat gataatggct catttacatt aacaagcgga
      961 atattaaatc gtgaccccat tctctatgga agttccgctg ccatggttaa tggcgcattg
     1021 gatggtttta tcaaaagtgc tgctctggaa atgccaagag gtatccgaat caatggtgta
     1081 agccccacag taattaccga agccatggaa aactatggcc cctatttcag aggctatgaa
     1141 cctgtatctg ctgctcgtgc agcactcgcc tacagcaaga gtattgaggg cgctcaaaca
     1201 ggtcaagtgt attgcgtagg gtaacattaa caggacttat gaaaaacccc aaggtcgagg
     1261 caaaaaatgt ttttaactag ggagttaaga taaactaaat gaccgaatta aaaatttttt
     1321 aacaaagaga ttggggttta ttgtaagccc tgattaaaat tatttaaaaa aacagcaagc
     1381 atggttttgc atagtattgc aggtaaaatt atgtttcttt atgtttttac acggttttat
     1441 agacgggacg aaagaaactc tttgatattt ctgatagtca attgcccctt ggccacaagt
     1501 gcctcccttg tattaaatcc tatgtgtggt gttgcgataa gattaggtac ctcccaaagg
     1561 ctataatttg caggtaatgg tggttcaaca tcaaatacat ccaatgcggc acctgctatc
     1621 atgtcctgtt ccaaggcttt ttttaaatca ctgttaacca caatcggccc tctggcacaa
     1681 ttaatcaaat acgctgattt tttcatttga gataaaagtc ttaagttaac catgcctttg
     1741 gtttcatttg taagtggcac atgtaaagtg acgaaatcag attgggaaaa caaatcgtct
     1801 aatgttactt gagatttcct gtcataaatt atagttgtca tttgaaatgc cttagcaagc
     1861 cgcgcaactt ctgcaccgat tgctccataa ccgacaatgc ccagcacttt attttttagt
     1921 tcaataccgg tattggttat gccttgctct cttattctct gattgttatc aggaatatga
     1981 cgtgcaagag aaatcatgag accaaatacc agttcagata ccgcagtgtt ggcataaccc
     2041 gctgcatttt ttaccggaat atttctttta ctcaccgcat ccctatcaat atggtcgatt
     2101 ccagcaaatg caacagcaat caattgtaat cgcagcgctg aattaatgac tgcagctgat
     2161 aaacgtcgat tcgtcagtgc aatgatgtct gcatccttca caaaatctat taattgctca
     2221 tcactccact gacgactgtc acactcgata atcgtgtggc ctggtaattc agaacgaatt
     2281 tcctgcatcg caatccctat aggctcaaca acggcaattt tcatgtaaaa tcctttaatc
     2341 caaatttaat taagtttttc cactccgctg tgacaagcaa tttgtcatcg ttatttcact
     2401 tcaatcaaag agatggctca ccattctata acgctctttt ttaaaaatta aggtaatgcg
     2461 aaatgccaat tcattgcttt ggataaacct tccagcatga tttcacctcg tatgctattt
     2521 cctttcgcat tcactcttgg actcaatacc acaatacctg ctttaccggg aacaaccgcg
     2581 atggtataac cagatactcc acttttagct ggcatccctg ttttaaccat gtgagtacct
     2641 gtttcatcat ataacccgca agtcgccata atggctaaag taattttaca ggtttccgta
     2701 gacagtattt gttcacccgt gtccggatct ttccctctat tagctaacaa agtaggtaag
     2761 tacagcatct gatccaacat ggcttcatag gaacatagcg caaaatacaa atcgagggtt
     2821 tcatgaacat cagctccaag attgtttctg cttttcagta aataagcaag agcgcggtta
     2881 cgattaccgg ttcgtttttc agaggcaaaa accagaggat taatactgag acgttgatta
     2941 aataattttt gtacccaatg ctctagccat ctaaattgct gttcacctat gccaggaatg
     3001 cgtgagcata aagcaatagc gccagcgttc aacatgggat ttgacggttt aggtccaaac
     3061 tgctccaggc gggtaatgga agcgaaatca tcacctgagg gctccacctt aacccattca
     3121 aataattgat cggcgccaaa ctcttcaagc aaaccaatca aaggaatcat cttccccgtg
     3181 ctttgcaaag tcactggatg aagtggatta ttactataag ccaaggtttt ctcacccaaa
     3241 gcttgcaccg caattgccgt caattcctga ttaacattcg ccagttcagg gatataatcc
     3301 gcggttttgc cttcttgatt taattcggca gcatgcacaa gctcttctaa caattggatg
     3361 gtaagcaatt ttgatgacat agtactattg gattaaaaaa ggggctatta tggtaaattt
     3421 agcacatcaa gacaaatatt ttattttgca agaatgctgt tagttaataa gtgaaggatg
     3481 aaaacttagt tcaagtaacg gtattgagaa aatatttttt gccttggtct tatgcatcaa
     3541 gcgaacaggt ctttgtccta caggcagaaa tccatttcta ctctgactac cagatttttt
     3601 gttcgctaaa gaattctgga aacaaaatga tgagggacac tatattggtc cattattttg
     3661 attgataaac aaactggttt ggtcgcttaa ctcgccagaa gttgcaagtg ctttaaagat
     3721 actcagtcga tgccctttgt ttacactcat gggcatggaa ttggcatgag aagatttctg
     3781 taacgtagta tttctcagat tttcatcaat gccctcaaga ttaaaatcag aatttaaatc
     3841 atcagcaaac tgcaaattta tcatgtgcgc ctctgcatcc ttcaccactt ggttaaaccc
     3901 ttccgaactc tcattaccta atcctttaca agcttcagta aaagattgaa tggttttcaa
     3961 atcattattt cctcttagaa tggaggtgaa atgcttgaag tcaatagcaa gctgatgatt
     4021 taaactgaca aaaacgtcca cttccagttg attgagaaac ttatagaaag atttgccctc
     4081 tttttctaca tcaaagaatt tcttaatttc tccaaggtat ggaataaaat ctgaattcca
     4141 tatagcaccg tcattacccg acttaatata gagtaaggtg agtcccaaaa tatatctgga
     4201 gaatttgacc aaggtgactg agccggattt ttcattttca aactttaaaa aagatttctc
     4261 aataaggccc aaaaagaatt ccatttcagg tttattgtct actcttttaa cagccaaaaa
     4321 cagcttgtct aaaaaaaact cgccatcttt tctacttgct tccggaagtt tttcaagcaa
     4381 gttgcgcatt atattttgat accacataat atcattcacc tgtaattaat tagagcaaat
     4441 gatatcaaaa actaattaag tgagtattaa gaataccttt ctcatttcaa acttcctagc
     4501 tattataccg acaatttttt caatccttct tcaataatcg aaaggccgag ttccaaatct
     4561 tttacgccag tagaaagtgg catgtggaga cgaatgacgt tcccgtaggt tccacaggtc
     4621 aaaataatca acccatgttc taaacaaaat tgggccaatt gtactgtggc ttctttatta
     4681 ggttctttcg tgtttttatc ctttaccaat tcgattgcct gcatgacacc caaaccacga
     4741 acatcaccaa ctaccttgta tttttcctta aatccagaaa gtctggactg cagtgccttt
     4801 gcaagatggg taacattttg cagcaggctt ccttcttcaa aaatatggaa cacttcaaga
     4861 gcagcagcac aactaagtgg atttccacca aaagttccac ccaacccacc ttccatagcc
     4921 gcatccatga tttcagcttt acctgttaca ccagcaagca ctaccccacc gccaaggcct
     4981 tttgctgata tggtgagatc ggggggtact cccaaagtat tcatcgcaaa cagatttcct
     5041 gtgcggccaa aaccggattg gatttcatcg gcaataaaaa cgatatcgtt tgtcgtacaa
     5101 aactcacgaa gtttttgcaa aaataaggca ggggattgaa taaatccacc ttcacccaat
     5161 accggctcta taatgactgc agcaatgttt tcaacaccga cacggaaatt ggcaagatcg
     5221 gtaaactcat cgaaacattc ttctacacaa ttggctccct tccaacgata ctcataagga
     5281 aaaggagcac gatggatttc agaaggaaac gggccgaatc catgcttata gggtttgttt
     5341 ttcgaagtga gtgtcattgc catgtacgtg cgtccatgaa aagcatgatc aaaacaaatc
     5401 actgcttgtt tgcccgtata ggcacgagca attttaatgg cattttcaac tgcttccgca
     5461 ccggaattca aaaggagcga ttttttttcg aaatgtcccg gggtgtgatc atttaatttt
     5521 tcacacacct tgatataaga ttcataagga ataatattga agccggtgtg tataaatttt
     5581 tcggcttgga gcttgatggc attgaccacc gaatcagggc agtgccctgt attgaccacc
     5641 ccaaaccctg atgaaaagtc caaaaataca tttccatcaa catcctccac aaaagaacct
     5701 ttcgcctgct tcacaaagat aggagtagca tgaaatggcc ctcttgcaac atgctgacgt
     5761 cgcaattcca tcaactgttg cgatttgggt ccgggaattg gcgttttgat atgaattttt
     5821 ttcattagtt tagtaccaac caattggtgt ttcatccaga ttaatattca cttgcttgac
     5881 ctcaaggtaa ctctcaatgc catacaaacc taactcacga cccgctccgc tttgtttgta
     5941 tccgccccaa ggcatttcat tataggttgg atggtaatga ttcacccaaa gaatcccggc
     6001 acggatttga gatgtgactc gatgggcccg agtaacgctc ttcgtccata cagccccaga
     6061 taaaccataa tcagtatcgt tcgcaatgcg aatggcctct tcttcagtgt caaaaggtat
     6121 cactacgagt actggaccaa aaatttcttc acgagcaatg cgcatcgtgg atttcacttc
     6181 atcaaaaatg gttggctcga aaaaatgtcc ctttgcaaat tcattgcctg tcgggcgttt
     6241 accgccgcaa agtaatttgg ctccttcttc cattccaagc ttgacatagc tttccacttt
     6301 ttcacgatgt gcacttgaaa ccaaaggccc catttttacc ccggcgtcca aaccatggcc
     6361 aagcttgata tttggaattt ttttcaacat tccctctact atttttttgt gaatagagcg
     6421 ctccactagc aagcgggaac ccgcagaaca gacttcccct tgatttgcaa aagcaccaaa
     6481 aagggcaccg tcgattgcca tttcaaggtc agagtctgca aagacaatat ttggattttt
     6541 tccgccaagc tctaacgaga tttttttcaa attgccagta gccgcctgca tgatttttct
     6601 gcctgttgcc gatcctccgg tgaaagcaac cttatccacc atagcattac ttgccaactc
     6661 ctctccaact cct
//
LOCUS       DACTJE010000169         6573 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.170, whole genome shotgun sequence.
ACCESSION   DACTJE010000169 DACTJE010000000
VERSION     DACTJE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.170"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..777)
                     /gene="leo"
                     /locus_tag="I9760_002578"
     CDS             complement(<1..777)
                     /gene="leo"
                     /locus_tag="I9760_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease Leo"
                     /protein_id="HAT5044327.1"
                     /translation="MKNKIVLLAPVSLSFLFSFSTYAADYEFNGLSLSASLGILSGKA
                     HEYVYHPETDTKLSQLDWRIKNAPIIKGELNYDVLDWLSMNGQGWTTLVKNKAAMDDY
                     DWLNPYQDTWTHWSHHEDTHLNYANELDMSLRAWLMQKQNYKLGLATGYQWNSFDWKA
                     TGGCYQYNGGLYAGCFPGDQPGIGYQQKFRTPYVGLAGRYFINNFEFSGVLKFSDWVS
                     ARDHDEHYARNLTFNERGNSSRYYAATIHSGYFLKPNTKIF"
     gene            complement(905..2512)
                     /locus_tag="I9760_002579"
     CDS             complement(905..2512)
                     /locus_tag="I9760_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAT5044328.1"
                     /translation="MNKNLSALSLLWISITSMVGSGWLFGSLYSAHFAGPAAIIAWPL
                     AGFLLLFVALSYAELGTMFPQSDSLACLPLYTHGRLTGIIMNVMTWFSLAILPVIETQ
                     GVIQYASNYIPDLVSHDALHYRNTPTGYMLSLLVLMSFILFNYFGIGLFARINAAFTI
                     WKIIIPILTIASLVTMSYHPGNFFQHGGFMPYGWQGVMAAMSSGGVLFSLLGFRQVVI
                     MMGELEKPGQYIPLILGGSLVFVTLLYTGLQWSFIGSVSEQALEKGWVNLSFTGDAGP
                     FAALAALAGMLWLSILLYADAFISPYSTGLVYSTTAAHMLSSMGSVVPTPVILTKQNR
                     YQVPWVSLGVNFLLAATMSLVLHGWQEMSAFLVAILMISYSIGPVALVCLRQQLPHYK
                     RPFRLQGSNLIGFLGFYVCTVGVYWAGFHSVRKLLVITILGVVCSFLYCLMKNTHREI
                     DSKQALWFIFYLFGLGIFSFLGNYGGKHILPQYWDLLYLLVFSLLVFGFALLSRKPSA
                     HTQNMVAQNTRTTITAPAFSIERYDIA"
     gene            2843..5008
                     /gene="katG"
                     /locus_tag="I9760_002580"
     CDS             2843..5008
                     /gene="katG"
                     /locus_tag="I9760_002580"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAT5044329.1"
                     /translation="MDGKVGSTTTGCPVIHGGMTSTGTSNTAWWPNALNLDILHQHDT
                     KTNPMEKDFNYREEVKKLDFEALKKDLHALMTDSQAWWPADWGHYGGLMIRMSWHAAG
                     SYRVADGRGGAGTGNQRFAPLNSWPDNVNLDKARRLLWPIKKKYGNKISWADLIVLAG
                     TIAYESMGLKTFGFGFGREDIWHPEKDVYWGSEQEWLGAKRYDGKSRESLENPLAAVQ
                     MGLIYVNPEGVNGQPDPLRTAQDVRVTFGRMAMNDEETVALTAGGHTVGKCHGNGNAK
                     LLGPNPEAANVEDQGLGWINKTTRGIGRNTVSSGIEGAWTTHPTQWDNGYFYLLLNYD
                     WELKKSPAGAWQWEPIHIKEEDKPVDVEDPAIRHNPIMTDADMAMKMDPVYRKIAERF
                     YQDPDYFAEVFARAWFKLTHRDMGPKTRYIGPDVPKEDLIWQDPVPAGNRAYDIAAAK
                     AKIAASNLTIGEMVSTAWDSARTFRGSDKRGGANGARIRLKPQKDWEGNEPQRLTKVL
                     QILEDIATDTGASVADVIILAGNVGIEKAAKAAGFDIIVPFAPGRGDATDDMTDAESF
                     DVLEPLHDGYRNWLKKTYDVRPEELMLDRTQLMGLTAHEMTVLVGGLRVLGTNHNNTQ
                     YGVFTDRVGALTNDFFVNLTDMANVWIPSKDNLYEIRDRKAGNIKWTATRVDLVFGSN
                     SILRSYAEVYAQDDNKGKFIQDFVAAWTKVMNADRFDLA"
     gene            complement(5785..>6573)
                     /locus_tag="I9760_002581"
     CDS             complement(5785..>6573)
                     /locus_tag="I9760_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5044330.1"
                     /translation="LGSICGLLLAIIVSKITMLFFSKQHLDSFWRFPFLIGIFVGFAG
                     LYLRSQTRELTDFAKVVAQRQSHPILNAWHYRKKAIAAIFLLAIYCNSSFYTFFVYMP
                     SYLQQQELPLTTAMDVIIIGMICYSMGLLLSGFTSDILGRRFPLAVSLIATLCTVLVI
                     NYLINTGTLLQITVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFNLSNSLF
                     GGTAPLIILLLSYAFIHFAFSIYLMVMALLTLPALWLIQEKSTN"
BASE COUNT         2106 a         1379 c         1344 g         1744 t
ORIGIN      
        1 aaaaattttc gtattaggtt tcaaaaagta gccagaatga atcgttgccg cataatatct
       61 ggagctattt ccccgctcat tgaatgttaa gttacgggca taatgctcat catgatctcg
      121 agctgaaacc caatcactaa attttaaaac accactaaac tcaaaattat taataaaata
      181 cctccctgcc aaaccaacat aaggagttct aaatttttgt tggtatccta ttccaggttg
      241 atctcctgga aaacaaccgg catagagccc tccgttatat tggtaacaac ctccggtagc
      301 tttccagtca aaggaattcc attgataacc agtagccagg cctaacttgt aattttgttt
      361 ttgcatcaac caagccctta agctcatatc taactcattt gcataattga gatgcgtatc
      421 ttcatgatga gaccagtgag tccaggtatc ttgataagga ttgagccaat cataatcatc
      481 catagcagct ttatttttta ccaaagttgt ccagccttga ccattcatgc taagccaatc
      541 caggacatca taatttaatt ccccttttat tatcggagca ttttttatgc gccaatccag
      601 ctggcttaac ttagtgtctg tctctggatg gtaaacatat tcatgagctt tacctgacag
      661 aatgcctaga gaagcactta atgacaaacc attaaattcg taatccgcag cataagtaga
      721 gaatgagaaa agaaaggata aagagactgg agccaacaaa actattttat ttttcatatt
      781 ttttcctagt taaggctggc aatccttgca gcaaagtgac tttaaaaaac caatcgcatt
      841 attgtgatcc aaaatgatct tgtcaagatc aacattgaat aatattaatc attaaggatt
      901 ctaatcaagc aatgtcatat cgctcaattg agaaagcagg agcagttata gtagtgcgtg
      961 tattttgggc aaccatgttt tgagtgtggg cactgggctt tctggacaat agggcaaaac
     1021 caaatacaag caagctgaaa actaataaat acaacaaatc ccaatactgg ggaaggatat
     1081 gcttaccacc ataattacca agaaatgaga aaataccaag tccaaaaaga tagaatataa
     1141 accacagtgc ttgtttgcta tcaatctccc gatgtgtatt tttcataaga caatagagga
     1201 aagaacaaac tactcccaga atagtgatca ccaataactt tcgtacgcta tggaaacctg
     1261 cccaataaac accgactgta caaacataaa atccgagaaa gccaataagg ttactcccct
     1321 gcaaacgaaa aggacgctta tagtgcggta attgctgacg taagcaaacc agtgctacag
     1381 gccctatgga atagctaatc attaatattg ccacaagaaa agctgacatt tcttgccatc
     1441 cgtgcaacac gagcgacatg gtggcagcga gtaagaaatt tacgcctaaa ctaacccaag
     1501 gaacttgata cctgttttgt tttgtaagaa ttacaggtgt aggaacaact gagcccatac
     1561 tacttagcat gtgagctgct gtggttgaat aaacaagacc agtggaataa ggtgatataa
     1621 aagcatcggc gtacaataaa atactcaacc aaagcatacc ggccagtgca gcgagagcag
     1681 caaatggtcc tgcatcacca gtaaaagaca aattcaccca ccctttttcc agcgcttgtt
     1741 cgctaacact cccaataaag gaccattgca aaccagtgta aagcagtgtc acgaacacta
     1801 atgaaccacc caaaatcaga ggtatgtatt gtcctggttt ctccaattca cccatcatga
     1861 taaccacttg tcgaaaccct aaaagtgaaa aaagcacacc tccgcttgac attgctgcca
     1921 taacgccctg ccagccataa ggcataaaac caccgtgctg aaaaaaatta cccgggtgat
     1981 aactcatggt taccaagctt gcgatagtaa gaatagggat tattatcttc cagatagtaa
     2041 atgcggcatt aatgcgggca aacaaaccaa taccaaaata attaaacaaa ataaaactca
     2101 ttaatacaag cagcgacaac atataacctg ttggcgtatt tcgatagtgc aaagcgtcat
     2161 gagataccag atctggaatg taattactag catattgaat gacaccttgc gtttcaatca
     2221 ctggcaaaat ggccagggaa aaccaagtca tgacattcat gataatgcca gtcaaacgtc
     2281 catgggtata taaaggcaag caagccaggc tgtcggactg tggaaacatc gtacctaatt
     2341 cagcgtaaga taaagcaaca aacagaagta aaaaacctgc cagtggccaa gcgataatgg
     2401 cagcaggacc agcaaaatga gcagaatata aagaaccaaa taaccagcct gagcctacca
     2461 tgctggtaat tgaaatccaa agtaaactca aagcacttaa atttttattc atctgatttt
     2521 cacatgtcct tatgattttg atgcaggcaa acaattcacc agtcagcaga gcgaaatcat
     2581 tatatttttg cattgtaaac agaaatatca tgactaccaa ccctataaga gtcaagttta
     2641 accaggtatg ttataaatac cttcctgcat caggaaaaat gaaaaacaag aacttgcagc
     2701 agtgattttc tttgctatgg attcagcatt tttcactctg caatctctcg cttgatagta
     2761 tccttgcttc gattgcttag ctgccctccc ttaagctata gttaggtctc tagtgaatta
     2821 tgatctgcga ccaggagtgt ctatggatgg aaaagttggg agtacaacaa ccgggtgtcc
     2881 ggtgatacat ggcggtatga cgtctacagg aacatccaac acggcatggt ggccaaatgc
     2941 cttgaacctg gatatattgc atcagcatga caccaagaca aatccaatgg aaaaagattt
     3001 caactaccgt gaagaagtca aaaaactgga ttttgaggca ctcaaaaaag atttgcatgc
     3061 tttaatgact gacagtcagg cgtggtggcc tgctgattgg ggacactacg gtggtctaat
     3121 gattcgcatg tcctggcatg ctgctggttc ctatcgcgtg gctgatgggc gtggcggtgc
     3181 aggtactggc aatcagcgtt tcgctccatt gaattcctgg cctgacaatg tgaacctgga
     3241 taaagcacgt cgcttgttat ggccgatcaa gaaaaaatac ggcaacaaaa tcagttgggc
     3301 agacttgatt gtcctggccg gtactatcgc ttatgagtca atgggactta aaacattcgg
     3361 ttttggtttt ggccgtgaag acatttggca tcctgaaaaa gatgtttact ggggttcaga
     3421 acaagaatgg ttgggcgcca agcgttacga tggcaaaagc cgcgagtctc tggaaaaccc
     3481 gctggcggca gtacaaatgg gattaattta tgtaaatccc gaaggagtga atggacaacc
     3541 tgatccgctt cgcacagcgc aggatgttcg tgtaaccttc ggacggatgg caatgaacga
     3601 cgaggaaacc gttgctctga ctgccggagg acatactgtt ggcaaatgtc atggcaatgg
     3661 caatgcaaaa cttttgggcc ccaatcctga agccgctaat gttgaggacc aagggcttgg
     3721 ctggattaac aaaaccacca gaggcatagg ccgtaatact gtttcaagcg gcattgaagg
     3781 cgcatggaca acacacccta cccagtggga caatggctac ttctatttat tgctgaatta
     3841 cgattgggaa ctgaaaaaaa gccccgccgg tgcctggcaa tgggagccca tccatattaa
     3901 ggaagaagat aaaccagtag atgttgaaga ccctgccatc cggcacaacc cgatcatgac
     3961 tgatgctgac atggcaatga aaatggatcc cgtttatcgt aaaattgcag agcgctttta
     4021 ccaagatccc gactattttg cagaagtttt cgctcgagcc tggttcaaac tgactcatcg
     4081 cgatatgggg ccgaagacgc gctacattgg ccctgatgtt cctaaagaag atttaatttg
     4141 gcaagatccg gtgcctgctg gtaacagggc atatgatatt gctgccgcca aagcaaaaat
     4201 tgcggccagc aatctgacca tcggtgaaat ggtttctact gcatgggaca gcgcgcgcac
     4261 tttccgcggc tcagataagc gcggtggtgc gaacggagca cgtatacgac taaagccaca
     4321 aaaagactgg gaaggcaatg agccacaacg cctgactaaa gtcttgcaaa ttcttgaaga
     4381 catcgctact gatacaggcg caagcgtggc agatgtgatt atacttgccg gaaatgtcgg
     4441 tatcgagaag gctgccaagg cagctggctt tgacattatc gttccctttg ctcctggccg
     4501 tggggatgca accgatgaca tgacggatgc agaatccttt gacgttctgg aaccgcttca
     4561 tgatggctat cgcaactggc ttaagaagac atatgatgtc cgtcctgaag agctcatgct
     4621 agatcgcact cagctcatgg gactgacagc ccatgagatg acagtccttg ttgggggtct
     4681 gcgcgtgttg ggtactaatc acaacaatac ccaatatggc gtattcactg atcgcgttgg
     4741 tgccctgact aatgactttt ttgtcaactt gaccgatatg gccaatgtat ggataccaag
     4801 caaagacaat ttgtacgaaa ttcgagaccg taaagcgggt aacatcaagt ggacagcgac
     4861 tcgtgtagat ctggtttttg gctctaattc gattctgcgc tcctatgctg aagtctatgc
     4921 ccaggatgat aacaagggaa aattcataca ggactttgtt gcagcatgga caaaggttat
     4981 gaatgctgat cgctttgatt tggcataatc ctttgcaagt tgtcatacca tagataatca
     5041 tagtagaaat agttccgatt atgtttagct ttgattgcct ttcatcgcat caaaactaaa
     5101 catagcgtac taccataaaa tgttgtttca aaagcgaaca tttatgatgg tttgcattag
     5161 gcctgattaa cctaacttgt ctcttttgtt ttatttctgc gttgcaaacg tttctgtaag
     5221 tctcatctac tgcatgtaaa ccccaatctt caaaacgctt gcgccctgcc ctaaaacaaa
     5281 atgatcagat catgcaagag gtctatttat attatgtctc agcacaaacg acatgcttca
     5341 gcatttatat cactgatggg ttgagttact cttgggatga aaattgttaa gaaccatatg
     5401 gattaacaaa ttaacatcac ggatattaaa agtttcatcc atatccagaa tattattatc
     5461 ttgaaaaatt atgtgatttc catcaaattt tactttaatt cttcggtgcc tgttttcacc
     5521 ttcttcaact tcaggtattt ctatttcaat tcttttgcta aatggattac cgtccagatc
     5581 aatatcttct ttatagtaag tgataatttt tgattgtttt tcataataat cgagcggtct
     5641 ctctgaagca aatccccaag gaatagggta gtgagattcg gtaataattt ctgtatcaat
     5701 accgtctatc tctttttttc cacataaata tattggctgg atttttgtgt tagatcactg
     5761 tttttcgtac cataaactaa taaattaatt cgtgcttttt tcttgaatca accacagggc
     5821 aggaagagtc aacaaagcca tcaccatcaa ataaatagaa aatgcaaaat ggatgaaagc
     5881 ataacttaat agcaaaataa tgagtggtgc tgtgcctcca aatagggaat tagacaagtt
     5941 aaaaaataaa gtaaagccac tggcccgctc ttcctgtggt aattgttcaa ggctggcggt
     6001 aaatgcactt ccaataaaaa aagagataat gacagccaaa acactgcagg cgacagtaat
     6061 ttgtaataaa gtacctgtat tgatcaaata atttattacc aaaaccgtac ataaagttgc
     6121 aattaaactc accgccaagg gaaagcgtct gcctaaaata tcagaggtga aacctgataa
     6181 taagagaccc atgctataac aaatcatccc tatgataata acatccatcg ctgtagttaa
     6241 tggcaactcc tgctgttgca ggtatgaagg catataaaca aaaaatgtat aaaagctact
     6301 gttgcaataa attgccaaaa gaaaaatagc agcaattgct tttttccgat aatgccaggc
     6361 atttaatatg ggatgagatt gtctttgagc aactactttt gcaaaatcgg ttaattctct
     6421 ggtttgactt cgcaaataaa gtccggcaaa acctacgaat attcctatta aaaatgggaa
     6481 gcgccagaag gaatcgagat gctgtttgga aaaaaacagc atggttattt tgctgactat
     6541 tattgcaagc aacagcccac aaatcgaacc caa
//
LOCUS       DACTJE010000170         6571 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.171, whole genome shotgun sequence.
ACCESSION   DACTJE010000170 DACTJE010000000
VERSION     DACTJE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.171"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..537)
                     /gene="rpe"
                     /locus_tag="I9760_002582"
     CDS             complement(<1..537)
                     /gene="rpe"
                     /locus_tag="I9760_002582"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAT5044331.1"
                     /translation="MTFQILPSLLSADMTRLGEEVDAVMKAGADFIHFDVMDNHYVPN
                     LTFGPAFCDALIKKFPNLPIDVHLMTTPVDTLIEAFAKAGAKRISIHPDATIHLDRSL
                     QLINNLGCEAGLVLNPATSIDTLTWCAHNLKFVLVMTVNPGFGGQKLIPEVIPKLSKI
                     RQQYPNLDLCVDGGVTPQN"
     gene            complement(751..1215)
                     /locus_tag="I9760_002583"
     CDS             complement(751..1215)
                     /locus_tag="I9760_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAT5044332.1"
                     /translation="MKALIAKTYLLADKIFDFLAPIFILLTRLYLAQVFFLSGLTKIS
                     NWQATLSLFQNEYMVPVMSPTLAAYLGTACELGFPVLITLGLGSRLVLFGLFLFNLFA
                     VFSYQFLWTPDGAVGLTDHMQWGIMILMLLCTGPGKLSIDYLIRRFYLKLDN"
     gene            complement(1212..1991)
                     /locus_tag="I9760_002584"
     CDS             complement(1212..1991)
                     /locus_tag="I9760_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HAT5044333.1"
                     /translation="MRNTLLETIQKEWIAQFKEEALSSPLKNRLARSNFDGISVYMES
                     HHATLFNHLKSTFPLCMRLVGENFWYALLHRYINNCESTQYDINQYGDSLPQFIANFE
                     PAKELPYLQQLCAIEWHWHTLKLLSPVNPFDFEAFSSIDEISFQSLCFHLTPNLKILC
                     SEYPIYQIWAMNYFQQDKQIQISGCPEKVVIYQHNDLPCIMLISEEEYIFLELLSSGN
                     QTPWQKVVTELSKWISEEKIGDTLTKALRYQWINHFSLEAI"
     gene            complement(1984..2853)
                     /locus_tag="I9760_002585"
     CDS             complement(1984..2853)
                     /locus_tag="I9760_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HAT5044334.1"
                     /translation="MYSNHSTDQAAPYSGCSGIGLRLEHIDNILKEQPTVDYFEVLAD
                     NYMKQSGAQFKQLLKIAEFYPMSLHSVGLSIATSSEPDYQYLQQIKNLAHCLNSKLIS
                     DHLCWTHANQFFTHELIPFPYTEETLSFIIEKTNRIQEYLNQPIMYENVSRYVTYRQN
                     TLSEAEFLNELSTATGCGILLDINNLYVNWYNHGDDPDKYLHSINTKNVWQMHLGGFS
                     KQEGYLLDSHSDKVYQDVWQLYEKAQNLFINTPTVIEWDNDLPDFSLLYDEMCKAKTI
                     IKRVAERSMNHYA"
     gene            complement(2859..3113)
                     /locus_tag="I9760_002586"
     CDS             complement(2859..3113)
                     /locus_tag="I9760_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HAT5044335.1"
                     /translation="MNNISKITMAAVMAAGLSIATIPANADTPNDMEKCQGVAKAGKN
                     DCGTAQHACAGQSKTDGSTTDWIYLPKGTCEKLVNGTVKS"
     gene            3450..4475
                     /locus_tag="I9760_002587"
     CDS             3450..4475
                     /locus_tag="I9760_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAT5044336.1"
                     /translation="MAKDTKMINLALQGGGAHGALAWGIIDRLLEDGRINFDAISATS
                     AGAMNAAVLAYGFATGGKEGAREALHQFWKMVSDAGQIYNPLKKTPLEELLGIGIENS
                     MSFFMFDLMTKILSPYQFNPLNFNPLKEILEKIVDFEKIKSCKSIKIFISATNVRTGK
                     INVFDNKKISADAVMASACLPFMFQAVNIGEDYFWDGGYMGNPAIFPLIYNSECRDIL
                     ILHINPIYREHVPDTAAEILNRVNEISFNSSLMREMRAIAFVTKLIDSGWIKDEYKNN
                     LRKLYLHAIRADVSMESASVASKLNPEWSFISHLYEEGRKKGEEWLKSNFKHLGKVTT
                     IDLDEYL"
     gene            4591..>6571
                     /locus_tag="I9760_002588"
     CDS             4591..>6571
                     /locus_tag="I9760_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT5044337.1"
                     /translation="MQQEDQAQIPQNSRSFSRNTARCFDTFMDTVDFLGRIGWPVIDL
                     AFRIWIAQQLLVSAILLSNHWDTAVYLAKNEYPVPWLSPEWEALLGILAQFVGGISLL
                     LGLFTRCGAAVIIGFAVMTQVYYQPIDLNLLWIALMLGYVLRGPGPLSLDNLFEHGFS
                     RSPLPFAMLIVTFVDRTREFFSSLYLFSLRMWLMISLLLINQHIQLISSDALPLLMPW
                     LPLNSASIVFNNVSLFMAIFLGLGLATRVVAIFGLIVVLLSFTPGVTGFYLYWMVLMA
                     ILFISGPGILSLDHLIFHALKKHYPQLSGKPAFSLDGLPRVVIIGAGFGGIACAKALR
                     HMPVKITLIDRHNYHLFQPFLYQIATGSLSPADIAISIRSIFLEQFNAEILLGNVTGI
                     NKEERLVIADNFTIPYDYLVIATGATHSYFGKDSWAPYAPGLKTINDGTSVRSRIIKS
                     FELAEIAESDEERKQFLNFVIVGAGPTGVELAGAIAELARFGIVKEFRHFDPASANII
                     LVQAAPRILPTFSEQISQKAQRYLESMGVKVLVNSMVEQIDSDGVIINKERIYSKSVF
                     WAAGVAASPASKWLQLEADPAGRVKVNDDLTVAGYSNIFAIGDTAASNAWNGKPVPGI
                     APAAKQGGAYVAKVISKRIYNNNSRYKPFKYIHYGS"
BASE COUNT         1941 a         1165 c         1352 g         2113 t
ORIGIN      
        1 attctggggt gttactccac catcaacaca aagatcgaga tttgggtact gttgccgaat
       61 tttagagagc ttgggtataa cctccgggat aagtttttga ccgccaaatc ctgggttaac
      121 tgtcatgacc aaaacaaatt ttagattatg ggcacaccag gttaatgtgt cgattgacgt
      181 agctgggttg agcactaaac ccgcttcaca tccaaggtta ttgattagct gcaaactcct
      241 gtctaaatga atagtagcat ctggatgaat actgatacgt ttcgcacctg ctttggcaaa
      301 tgcttcgatt agagtatcaa ctggagtggt catcaaatgg acatcaatcg gcaaatttgg
      361 aaattttttg attagagcgt cacagaaagc ggggccaaag gtcaggttgg gtacataatg
      421 gttatccatg acatcaaaat gaataaaatc agcgcctgct ttcataacgg catctacttc
      481 ctctcctaaa cgggtcatgt ccgctgacag taaagagggt aaaatttgga atgtcatgat
      541 ttagtaattc aacggtttgc ttttacatat aataagctga ctttggtaaa cattccagca
      601 ttacgatttg gattcatatc gagttttatt gttttaatcc tggatttgag ttggagcaaa
      661 tcaagataat aatactccat cctctctgat tcgttagaga ggatggtata gaatgcttga
      721 tgtttcgaac tatggagttt aaagattcaa ttaattgtct aatttcaaat aaaatcgtct
      781 gattaaataa tcaattgata atttccctgg cccggtacat aaaagcatta aaatcatgat
      841 tccccattgc atgtgatcgg taagaccaac ggcaccatcc ggtgtccata ggaattgata
      901 agagaaaacg gcaaataagt taaatagaaa aagtccaaat aaaacgagtc tggatccaag
      961 tcctaatgta atcaatacag gaaagcccag ttcacaagct gttcccagat acgcagctaa
     1021 agtaggcgac atgaccggaa ccatgtattc gttttggaat aaagaaagag tagcctgcca
     1081 attactgatt ttggttaagc cagatagaaa aaaaacctgg gcaagataaa ggcgggttaa
     1141 aaggataaaa attggggcaa ggaaatcaaa aattttatca gccagcagat aggtttttgc
     1201 tataagtgct ttcatatagc ctccagtgaa aaatgattaa tccattgata acgaagcgcc
     1261 ttggttagag tgtcaccaat tttttcttcg ctaatccatt tacttaattc tgttaccact
     1321 ttttgccatg gtgtctggtt tccagaggat aagagttcca gaaaaatata ttcctcctca
     1381 ctaattagca taatacaagg taaatcatta tgctgataaa taacaacttt ttcaggacaa
     1441 cctgatattt gtatttgttt atcctgctga aaatagttca tagcccaaat ttggtaaata
     1501 ggatattcag aacagagaat ttttaaattg ggtgtcagat gaaaacataa agactgaaag
     1561 ctaatctcat ctatgctcga aaaagcctca aaatcaaaag ggttaacagg ggaaagaagc
     1621 tttaaggtgt gccagtgcca ctcaatagcg cataactgct gcaagtaagg cagctcttta
     1681 gccggctcaa aattcgcaat aaattgagga aggctatctc cgtactgatt aatgtcatat
     1741 tgagtggatt cgcaattatt gatatagcga tgcaataaag cgtaccagaa gttttccccg
     1801 accaaacgca tacagagagg aaaagttgat ttcaaatgat taaataaagt agcatggtgg
     1861 ctttccatgt agacactaat accatcaaaa ttggatcgag cgagcctgtt ttttaagggg
     1921 cttgatagtg cctcctcttt gaattgtgct atccattcct tttgaattgt ttcaagcaga
     1981 gtattacgca taatgattca tactcctttc tgccacacgc tttattatgg tttttgcttt
     2041 acacatttcg tcatacagga gtgaaaaatc tggcaagtca ttatcccact cgataactgt
     2101 tggcgtgttt ataaagagat tttgagcctt ttcataaagt tgccaaacgt cttggtatac
     2161 tttatcacta tgactatcca gcaaatatcc ctcttgtttt gaaaatcccc caagatgcat
     2221 ctgccatacg tttttggtat tgatcgaatg aagatactta tccgggtcat caccatgatt
     2281 ataccaattc acgtataaat tattgatatc caataagata ccacaacctg tagcagtact
     2341 taactcattg agaaactcgg cttcagataa tgtattttgt cgataagtga cgtatcttga
     2401 tacgttctcg tacattattg gctggtttag gtattcctga atgcggtttg ttttttcaat
     2461 aatgaatgag agcgtttctt cagtataggg aaacggtatc aattcatggg taaaaaattg
     2521 attggcatgg gtccagcata aatgatctga tattagcttg gagtttaaac aatgagccaa
     2581 attttttatt tgctgcaaat attgataatc aggttcagag gatgtggcga tagataagcc
     2641 aacgctgtgt aaactcattg gataaaactc tgcaatcttg agcaactgtt taaattgagc
     2701 tcccgattgt ttcatgtagt tatcagccag gacttcaaaa taatcgactg tgggctgttc
     2761 ctttaaaata ttgtcgatgt gttcaagccg caatccaatg cctgaacatc cagaataagg
     2821 cgcggcttga tcggtagaat gatttgaata cattctaatt agcttttaac tgtgccgtta
     2881 accagctttt cacaagtacc tttgggcaga taaatccagt cagttgttga accatctgtt
     2941 ttagattgac cagcacaggc atgttgagct gtaccacaat cattttttcc agctttggcc
     3001 acaccttggc atttttccat atcatttgga gtatctgcat tagcaggtat ggtagctatt
     3061 gaaagtccag cagccatcac ggcagccatt gttattttag aaatattatt catgatacct
     3121 atcctttgtt atatgtaatt aatccatatg ttatttaata aaccccttgc ctaaccgggt
     3181 tttttcattg tattcctgta tgcaaacaca gctcctcaaa atgtttgttt cttgctctaa
     3241 aaaacaatca ggttaggcaa gaggtctaat aaaataacgt agttagtata gtaccatttt
     3301 tcgatttgtg acaggacatc aacttatttc tcaaagatgg aaaaattttt aacatggttt
     3361 gtttttattt caaacaggat agaaaacatt tcttagatct ttgccttata cttaataaat
     3421 cacagcaaaa ggaagaggag cattgtatca tggcaaagga taccaaaatg attaatcttg
     3481 ctttacaagg aggtggtgct catggtgctc ttgcctgggg tattattgac agacttcttg
     3541 aagatggtcg tattaatttt gatgccattt ctgcaacgag tgctggtgct atgaatgctg
     3601 ccgtacttgc ctatggattt gcaaccggcg gaaaggaggg ggctcgagaa gctctgcatc
     3661 aattttggaa aatggtgagc gatgccgggc agatatacaa cccacttaaa aaaactcctt
     3721 tggaagaact attgggcata gggattgaaa attcaatgtc cttttttatg tttgatttga
     3781 tgaccaaaat tctttctccc tatcaattta atcccttaaa ctttaaccca cttaaggaaa
     3841 tattagagaa aattgttgat tttgaaaaaa ttaaatcctg taaaagcatt aaaatattta
     3901 tctcagctac taatgttcgc acaggtaaaa ttaatgtatt tgataataag aaaatttctg
     3961 ctgatgcggt tatggcctct gcttgcctgc ctttcatgtt tcaggcagtg aatattggcg
     4021 aggattattt ttgggatggt ggttatatgg ggaatccagc tattttccct ctgatttata
     4081 attccgagtg cagggatatt ttaattcttc atatcaatcc tatttatcga gagcatgtcc
     4141 cggatactgc agccgaaatt ctaaatcgag tgaatgagat cagttttaat tcttcattaa
     4201 tgcgggaaat gcgggcgatc gcttttgtta ccaaattgat agacagtggt tggattaagg
     4261 atgaatacaa aaacaacctc aggaaacttt atttacatgc cattcgcgca gatgtatcaa
     4321 tggaatccgc ctcagtagcc agtaagctta accccgagtg gtcttttatt agtcatcttt
     4381 atgaagaggg acgcaaaaag ggagaagaat ggttgaagag taattttaaa catcttggaa
     4441 aagtgaccac tattgatctg gatgaatatt tataatttta ttatcagaat gatagtccac
     4501 aacataattt gaaatttgca gctataatta aatccaaagt tattagaaat acaaagatat
     4561 tttaacaact ttcttgaaag ggattttgat atgcagcaag aagatcaggc acagatacct
     4621 caaaacagtc gttcgttttc aagaaacacg gcgaggtgtt ttgacacttt tatggacact
     4681 gttgactttc ttggccggat tggatggcct gttattgatt tggcatttcg aatatggata
     4741 gcccagcaat tattagtttc tgcaatactt ctaagcaacc actgggatac tgctgtttat
     4801 ttagctaaaa atgaataccc ggttccatgg ctatcacctg aatgggaagc tttgttaggc
     4861 atattggcgc agtttgtagg ggggatttcc ctgcttttgg gtttgtttac ccgatgcgga
     4921 gcggcagtta ttattggatt cgctgtcatg actcaagtct attaccagcc tatcgatctt
     4981 aatttgcttt ggattgcatt aatgttgggt tatgtgttgc gaggcccggg tccactttct
     5041 cttgataatt tgtttgagca tgggtttagc cgtagccctt tgccgtttgc catgttgatt
     5101 gtgacatttg tagataggac aagagaattt ttttcaagtt tgtatttatt ttctttacgt
     5161 atgtggttaa tgatttcact tttactcatc aatcaacaca tacagctgat ttcttctgat
     5221 gcgctgccgc tattgatgcc atggttgcca ttaaattcag cctctatcgt ttttaacaat
     5281 gtttcactat tcatggctat ttttttggga cttggcttag cgactcgagt tgttgcaatt
     5341 tttggattaa tagttgttct tttgagtttt acgcccggag taaccggttt ttatttatac
     5401 tggatggtgt tgatggctat tttatttatt tcgggtccag ggatcttgtc attggatcat
     5461 ttgatctttc atgctttgaa gaaacattat cctcagcttt ctggaaaacc ggcatttagc
     5521 ctggatggtt tgccacgagt agttattata ggcgcaggtt ttggggggat tgcttgtgct
     5581 aaggcactac gtcatatgcc agtcaagatt actttgattg atagacacaa ttatcatctt
     5641 tttcaacctt ttttatacca gattgctact ggaagtttat caccagccga tattgctatt
     5701 tcgattcgct ctatattttt agaacaattt aatgcggaaa tattattggg taatgtaaca
     5761 ggtattaata aagaggaacg tcttgtcata gctgataatt tcacaattcc atatgattat
     5821 ctggtcatcg ccacaggagc aacgcacagt tattttggaa aggacagttg ggccccctat
     5881 gctcccgggt taaaaacaat taacgatgga acttctgttc gtagccgtat aataaagtcg
     5941 tttgaactgg ccgagatcgc tgaaagtgat gaagaacgca agcaattttt aaattttgtc
     6001 attgtaggtg ccgggcctac tggagtggaa cttgcaggag ccatagcaga actggctcga
     6061 tttgggatag taaaggagtt tcgtcatttt gacccggcat cagcaaatat tattctggtt
     6121 caggcagcac cgcggatttt gcctaccttt tcagaacaaa tatcgcaaaa agcgcaacgc
     6181 tatttggaaa gcatgggggt taaagtttta gtgaatagta tggttgagca aattgatagc
     6241 gatggtgtga ttattaataa ggaaagaatt tattcaaaat cagttttttg ggcagcaggt
     6301 gttgctgctt cccctgcatc aaaatggctt cagctcgaag ctgatccagc gggtcgcgtg
     6361 aaagtgaatg atgatctcac cgtagccggc tattctaata tttttgctat tggggataca
     6421 gctgcttcga atgcctggaa tggcaagccg gttcctggta ttgctcctgc tgcaaaacag
     6481 ggcggtgctt atgtagcaaa agtcatatcc aaaagaattt ataataacaa ttcaagatac
     6541 aaaccattta aatatattca ttatggtagc t
//
LOCUS       DACTJE010000171         6556 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.172, whole genome shotgun sequence.
ACCESSION   DACTJE010000171 DACTJE010000000
VERSION     DACTJE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.172"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(91..534)
                     /gene="tadA"
                     /locus_tag="I9760_002589"
     CDS             complement(91..534)
                     /gene="tadA"
                     /locus_tag="I9760_002589"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAT5044338.1"
                     /translation="MIDKFWMQLAYEQAVIARNEGEVPVGAVLVSKDNQLLGVGRNVI
                     EKSHDPSDHAEIRAIRQASRKLNNHRLLDTTLYVTLEPCVMCAGLMVHARIKRLVFAT
                     RDFKTGAAGSMFNVLHALNHSVLIDEGIMQAECSQLLSDFFRNLR"
     gene            complement(531..2108)
                     /gene="guaA"
                     /locus_tag="I9760_002590"
     CDS             complement(531..2108)
                     /gene="guaA"
                     /locus_tag="I9760_002590"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAT5044339.1"
                     /translation="MNDLKKSPLLILDFGSQYTQLIARRVREMGVYCEIYPYHINHEQ
                     FKKLNPCGVILSGGPSTVTHDANPRAPQWLFDSGLPLLGICYGMQTMAVQLGGQVHSS
                     ALREFGYAELRLHGHSQLLNNIEDRILADGSALLDVWMSHGDKVTELPPGFKIICETR
                     NAPIAGMADESRQMYGLQFHPEVTHTLQGLRILQRFVVDICKASTDWTPEHIIDEAIN
                     KIREQVGTEKVLLGLSGGVDSSVVAALLHRAIGEQLVCVFVDTGLLRLNEAEQVLSMF
                     GRHMGIRIIAVNAEDKFLTALKGVTCPEEKRKIIGRTFIEVFDEEAQKLTEIKWLAQG
                     TIYPDVIESAATSTNDAAVVIKSHHNVGGLPDTLNLKLLEPIRELFKDEVRQVGLELG
                     LPHDMVYRHPFPGPGLGVRILAEVKKEYADILRKADAIFIEELHNAQLYHKISQAFAV
                     FLPVKSVGVMGDGRRYDYVICLRAVETVDFMTAHWSQLPWDFLGKVSNRIINEVEGVS
                     RVTYDISGKPPATIEWE"
     gene            complement(2111..3583)
                     /gene="guaB"
                     /locus_tag="I9760_002591"
     CDS             complement(2111..3583)
                     /gene="guaB"
                     /locus_tag="I9760_002591"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAT5044340.1"
                     /translation="MPLSIVQQSLTFDDVLLVPAHSTILPKDVSLKTKLTRAIHLNMP
                     LISAAMDTVTEARLAIALAQEGGLGIIHKNMSIADQAEEVRRVKKFESGMVKDPISVT
                     PDLTVKELLAVMTKYNFSGVPVVDGKHLVGIVTSRDIRFETNMNLTVAQVMTPKGRLV
                     TVKEGASREEVRSLLHKHRIEKLLVVNENFELRGLITVKDIQKAKDNPYACKDSFEQL
                     RVGAAVGVGEGTDERVAALVESGVDVIVVDTAHGHSQGVLNRVKWIKKNYPDVQVIGG
                     NIATAAAARDLYEAGADAVKVGIGPGSICTTRIVTGVGIPQISAIANVAQELKGIIPV
                     IADGGIRFSGDVCKALAAGADTVMLGSMFAGTEESPGEIELYQGRTYKNYRGMGSIGA
                     MSLAQGSSDRYFQDASLGTEKLVPEGIEGRVPYKGPVQTIIHQLLGGLRSCMGYTGCA
                     TIEELHSKTEFVQVTNAGMRESHVHDVSITKQAPNYQVDN"
     gene            3792..4622
                     /gene="minD"
                     /locus_tag="I9760_002592"
     CDS             3792..4622
                     /gene="minD"
                     /locus_tag="I9760_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAT5044341.1"
                     /translation="MAKIIVITSGKGGVGKTTSSAAISSGLALLGHKTVVIDFDIGLR
                     NLDIIMGCERRVVYDFINVINGEANLNQALIKDKRIPNLCILPASQTRDKDALTLEGV
                     EKTLNELAKDFDFIICDSPAGIETGALMAMYFADHAIVVTNPEVSSVRDSDRILGILA
                     SKTKRAIENKAPVQEHLLLTRYDPERVERGEMLSVTDVKEILAIPLIGVIPESKSVLK
                     ASNTGTPVILDESSDAGIAYQDAIARFLGEERPMRFISIDKKGLFRRLFSKNKEEVTI
                     "
     gene            4619..4888
                     /gene="minE"
                     /locus_tag="I9760_002593"
     CDS             4619..4888
                     /gene="minE"
                     /locus_tag="I9760_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAT5044342.1"
                     /translation="MSIFNYLRRRASTASVAKERLQIIISHERSQRNTPDYLPKLQEE
                     ILAVIAKYVNISRDQVSVNLERMEDSAVLELNITMPDKALEDSNS"
     gene            complement(5203..>6556)
                     /locus_tag="I9760_002594"
     CDS             complement(5203..>6556)
                     /locus_tag="I9760_002594"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT5044343.1"
                     /translation="AISLPSSASGGAQAVALASGAYARIRKQFNQPIGKFEGIEEPLA
                     RIASAAYIIDSSLTMAAAAIDNGAKPSVAGAILKYHTTERARQLALDGMDIHGGKGIC
                     LGPNNYIGRGYQGSPIGITVEGANILTRSLIIFGQGAIRCHPYVFKELESIRNNDLVA
                     FDDSFFAHVGFVITNFTKSLIFGWTDARFSKSPASSVKRYYQLIHKYSSNLAFLADFS
                     MIVLGGELKRKEKLSARLGDMLSCLYMASAVLKRFYEDGEPRADLPVVHWSCQQLLHE
                     CETAMHGVIINFPARWARIVLSLILKPLGNRRHKPDDKLGHKVARILIEPNETRNRIT
                     RLVYKKPGDNCPLGRMEEAFHKICAVEELERKVMKAVKDNILHSLTFQEQILEALEHQ
                     ILTPVEAKQLEEAEKARQDVIRVDDFADEELRRPSSLGEKVKSEKKIPARDDMETETA
                     "
BASE COUNT         2012 a         1462 c         1128 g         1954 t
ORIGIN      
        1 ctgaatcatg aggaaaaaac aattttaaaa gaaaaattaa ttttggaaat ttctaaaagc
       61 aagtattaag aaatatgcca agataaatta ctagcgaagg tttctgaaaa aatctgacag
      121 caattgagaa cactcagcct gcataatgcc ttcgtctatt aataccgaat ggtttagagc
      181 atgtaaaaca ttaaacattg agccagctgc ccctgtttta aaatctcggg tagcaaatac
      241 cagccgtttg atgcgtgcgt gaaccataag tcccgcacac atcacacagg gctcaagagt
      301 aacataaagc gttgtatcca ataatctatg attatttaac tttctcgagg cttgtcgtat
      361 tgccctgatt tcagcatgat cagaaggatc atgacttttt tcaatgacat ttctgccaac
      421 gcctaataat tgattgtctt tgctaaccaa cacagcccca acaggaacct ctccttcatt
      481 acgagctata accgcttgct cgtaagccag ttgcatccaa aatttatcaa tcattcccat
      541 tcaatggtag caggtggctt gccagaaata tcgtaagtga cacgtgatac tccctctact
      601 tcattaataa ttcgattgga tacttttcct aaaaaatccc agggcaattg agaccaatgt
      661 gctgtcataa aatcaactgt ctcaacagct cttaaacaaa ttacgtaatc gtatcggcgt
      721 ccatccccca tcacacctac gctctttact ggtaaaaaaa cagcaaatgc ctggctgatt
      781 ttatgataaa gctgcgcgtt atgcagttct tcaataaaaa tagcatcggc tttacgtaat
      841 atatccgcat attctttttt tacttccgct aaaattctta cacccaatcc aggtccagga
      901 aagggatgcc gatagaccat atcatgtggg agtcccaatt ccagacctac ttgacgtact
      961 tcatctttaa agagctcacg aataggttcc agcagtttaa gatttaatgt atctggtaag
     1021 ccaccgacat tatgatgaga ctttatgacc acagccgcat cattagtact tgttgctgca
     1081 gactcgatga catcgggata gattgtgccc tgagccagcc actttatctc agtcagtttc
     1141 tgtgcttcct catcaaagac ttcaataaat gtacgcccaa taatttttct cttttcctct
     1201 gggcaagtta ctcccttcaa agcagttaaa aatttatctt cggcattgac tgcaataatg
     1261 cgtattccca tatgccgacc gaacatgctc agcacttgct ccgcctcatt aagtctgagt
     1321 aaacccgtat caacgaaaac acaaaccagt tgctctccaa tggctctatg cagcaatgcg
     1381 gctacaacag aagagtcaac gcctccggat aggcccagta aaactttttc agttcctacc
     1441 tgttctctga ttttattaat ggcttcatca ataatatgct ctggagtcca gtctgttgat
     1501 gctttacaaa tatctacgac aaaacgttgt aaaattctta aaccttgcaa ggtatgagta
     1561 acttctggat gaaattgcaa accatacatt tgacgactct catcagccat acctgcaatt
     1621 ggcgcattgc gtgtttcaca tataatttta aatcctggcg gtaactcggt aactttatct
     1681 ccgtggctca tccatacatc caatagtgcg ctgccatccg ccaggatgcg atcttcgata
     1741 ttatttaata attgactatg gccatgcaag cgcaactcgg catacccaaa ctctcttaac
     1801 gccgatgaat gcacttggcc accgagttgt acagccatgg tttgcatccc gtagcaaata
     1861 cccagcaagg gcaaaccact atcaaatagc cattgaggag ccctgggatt ggcatcatgc
     1921 gttactgtcg aaggaccccc tgataaaatc acaccgcagg gatttaattt cttaaattgt
     1981 tcatgattaa tatgataagg ataaatttca caataaaccc ccatttctcg aacgcgtctt
     2041 gcaattaatt gggtgtattg agaaccaaaa tctaatatta ataatgggct ttttttcaaa
     2101 tcattcatta ttaattatcc acctgataat tgggggcttg cttagtaata ctcacatcat
     2161 gcacatgtga ctcacgcata ccagcattag tcacttgcac aaattcagtt ttagaatgca
     2221 attcctcaat agtagcacaa ccggtatagc ccatacagga gcgtaaacca cctaataatt
     2281 gatgaataat tgtttgaacc ggtcctttat aaggtactct tccttcaatg ccttcgggaa
     2341 ccagtttttc tgtaccaaga gaagcatcct gaaaatagcg gtcacttgac ccctgagcta
     2401 atgacatggc tccaatggat cccatacctc tgtaattttt ataagtacgc ccctgatata
     2461 attcaatttc accaggagat tcttccgtac ctgcaaacat actgccaagc attacagtat
     2521 cagcaccggc agccaacgct ttacaaacgt caccagaaaa acgtattcct ccatcggcta
     2581 tcacaggaat tatcccttta agttcttgtg ccacatttgc tatagcgcta atttgcggga
     2641 taccaacacc ggtcactatg cgagtggtac aaattgatcc tggcccaatt ccaactttta
     2701 ctgcatcagc acccgcctca tataaatctc ttgcggcggc agcggttgca atattaccac
     2761 ctatgacttg tacatcagga taattctttt taatccattt cacgcgattt aatacgccct
     2821 gagaatgacc atgcgctgta tccactacta tgacatcaac tccggattca accagagctg
     2881 caactcgctc atcagtccct tcaccaacac caacagcagc cccaactctt agctgctcaa
     2941 aactgtcttt acaagcatag gggttgtctt ttgctttttg tatgtctttt actgtaatga
     3001 gtccacgtaa ctcaaaattc tcattcacta ccaataattt ttctattcgg tgcttatgga
     3061 gcaaacttct gacttcttct ctactcgctc cttcttttac agtaactaaa cgccctttgg
     3121 gagtcattac ttgcgccaca gtcaaattca tattggtttc aaaacgaata tctcgactgg
     3181 ttactattcc taccaaatgc ttaccatcaa ccacaggcac ccctgaaaaa ttatattttg
     3241 tcatgactgc aagcaattct ttaactgtca agtcgggagt tactgaaata ggatctttca
     3301 ccataccgct ttcaaatttt tttactcttc tgacttcctc tgcttgatca gcgatactca
     3361 tattcttatg aataatcccc aaaccacctt cctgtgctaa agcgatagct aacctcgctt
     3421 cggtcactgt atccattgcc gccgaaatta aaggcatatt aaggtggatt gcacgggtta
     3481 atttggtttt caatgaaacg tctttgggta aaatagttga gtgggcaggg acaagcaaaa
     3541 catcgtcaaa ggttaatgac tgttgcacaa tggatagagg catttttatc tcactccagt
     3601 aattaaataa ccaaatagtt tactacaaac tttaaaaaat ttaaacattt attgtataaa
     3661 tatatcacta caacctttta attagttttt ataacaatta agctttggat aacttgaatt
     3721 aatgaatgaa taacgtgtaa actgttggtt aaatattgtg ttgaatcgaa aaaaatagga
     3781 agggttcagc gttggctaaa ataatagtaa ttacatctgg gaaaggcgga gtaggtaaaa
     3841 ctacttcatc agcagcgatt tcttcgggac ttgcactctt agggcataaa actgttgtta
     3901 ttgattttga tatcggttta agaaacctgg atatcattat gggctgtgaa agacgtgtag
     3961 tttatgactt catcaacgtc atcaatggtg aagccaactt aaaccaagct ctcatcaaag
     4021 acaaacggat ccctaacctt tgcatactgc ccgcatcaca aactcgtgat aaagacgcac
     4081 taactctgga aggagtagaa aaaactctca atgagctagc aaaagatttt gatttcatta
     4141 tttgtgactc ccctgctgga attgaaactg gagcacttat ggcaatgtat tttgctgatc
     4201 atgccattgt tgttactaat cctgaagtct cttctgttag agattcagat agaattctgg
     4261 gaatactggc cagcaaaaca aaaagagcta tagaaaataa agcacctgtt caagagcact
     4321 tgttattgac acgatatgat cctgagcgag tagaacgagg tgaaatgttg tctgtaactg
     4381 atgtaaagga aatattagct attcctttaa tcggtgttat acctgagtcc aaatcggttc
     4441 ttaaagcatc caatactggt acgccagtta ttctggatga atcaagcgat gcgggcattg
     4501 cttatcaaga cgccatagca cgctttttgg gcgaagaaag accaatgcga tttataagta
     4561 ttgataaaaa aggactattt cgtcgattat tcagtaaaaa caaggaggaa gtgacaatat
     4621 gagtattttc aattacttac gcagaagagc ctcaaccgct tctgttgcta aagaacgctt
     4681 acaaatcatc atttctcatg agcgttctca acgcaataca ccagactatt tgcctaagct
     4741 gcaagaagaa attcttgcgg ttatagcaaa atacgtgaat ataagccgag atcaagtgag
     4801 tgttaatctg gagcgcatgg aagatagtgc cgttcttgag ctaaacatta caatgccaga
     4861 taaagcattg gaagacagca actcataatt ctattaagtt ttttatactt ataggtatta
     4921 ttacactatc aaataatgta gtttacctgc ctgcttaaca tacgcattaa aacataatct
     4981 ccttcaagaa tatttcaaaa aactttaaat aaaataccta aatgagttaa gcattacatg
     5041 catgggagtt aaactcattc ttaatgactc tgcctgtaaa gatcaattga ttaaaaccaa
     5101 aatcgataaa aacatatggg aatgattagc ctgactctaa ttgctgaata gaattacatc
     5161 atgtaattct attcagcatt atagaaaaga atctagtata aattaagctg tttcagtttc
     5221 catgtcatcc ctggctggaa tctttttttc tgactttacc ttctcaccaa gtgacgaggg
     5281 acgacgtagt tcttcatcag caaaatcgtc tacccttata acatcctgtc tggccttctc
     5341 agcctcctct agttgtttcg cttcaactgg agtcaatatt tgatgctcaa gcgcttccaa
     5401 tatttgctcc tggaaagtca atgaatgaag gatattatct ttaacagcct tcatcacttt
     5461 gcgctctagc tcttcaactg cacaaatctt atgaaatgct tcctccatcc ttcctaaggg
     5521 acaattatcc cctggcttct tataaaccaa tctcgtgatt cgatttcttg tttcattagg
     5581 ctctatcagt attcttgcta ccttatgacc caatttatca tccggtttat gccttctgtt
     5641 gccaagaggt tttagaatta acgataaaac aattcttgcc cagcgtgccg gaaaattaat
     5701 gattacccca tgcatagcgg tttcacactc atgcaataac tgctgacaac tccaatgaac
     5761 aacaggtaaa tcagcacgag gttctccgtc ttcgtaaaat cgtttcaaaa cagcggatgc
     5821 catgtataaa caactcagca tgtcacctaa gcgtgccgat aatttttctt ttcgctttaa
     5881 ctcaccacct aatacaatca ttgaaaaatc tgctaaaaaa gccagattgg aactgtactt
     5941 atgaattaat tgataatatc tttttacaga actggctgga gacttgctaa aacgagcatc
     6001 ggtccatcca aaaataagtg actttgtaaa attagtaata acaaaaccca catgggcaaa
     6061 aaaggaatca tcaaacgcaa ctaaatcatt atttctaata ctttctaact ccttgaaaac
     6121 ataaggatga caacgtatcg ctccttgccc gaatataatt aagcttctgg ttaaaatatt
     6181 ggccccttcc accgtaatgc caataggaga accttgatat cctcttccaa tataattatt
     6241 tggaccaagg catatccctt taccaccatg tatatccatt ccatccagtg caagttgtct
     6301 cgctctttca gtagtatgat actttaaaat agcccctgcg acagaaggtt ttgctccatt
     6361 atctatagcc gctgctgcca tagttaaaga agaatcaatg atataagcag ctgaggctat
     6421 tctggcaaga ggttcttcta tcccctcgaa cttgccgatt ggttgattga attgtttacg
     6481 gattctggca taggcaccag atgctaatgc aacggcttgt gcacctccac tggcgcttga
     6541 tggcaaagag attgca
//
LOCUS       DACTJE010000172         6521 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.173, whole genome shotgun sequence.
ACCESSION   DACTJE010000172 DACTJE010000000
VERSION     DACTJE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.173"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            37..591
                     /locus_tag="I9760_002595"
     CDS             37..591
                     /locus_tag="I9760_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAT5044344.1"
                     /translation="MNLIKIKSKTLFMVLVYSLLSPAYAASHPKSTVNDTAIQNEILV
                     YINNYRQQHGLAPLKMDNRIVKEAKIHSMDMAKHAIPFGHKYFKKRIDKLHTQIKNSN
                     AGAENVAYNYKNAQDVVKNWLRSPGHKRNIVGNYDLTGVGIARDEKGKIYFTQIFLKT
                     GNSHYASRKPFPSIFSGALFSKKA"
     gene            985..2118
                     /locus_tag="I9760_002596"
     CDS             985..2118
                     /locus_tag="I9760_002596"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAT5044345.1"
                     /translation="MMIATLMESAQETRVAITPNSAKQYIKLGYDVGIEKNAGLSSDF
                     ANEEFEKAGAIIFDSKSTLLKKTQILLCVNEPDPKNLNGLPPDSLIIGHIDNNPLSPL
                     VKWCLDQKMTLFSMNLIPRISRAQSMDSLSSQANLAGYRAVLEAAATFHRAIPMMMTA
                     AGMIQPAKALILGAGVAGLQAIATAKRLGAVVYAFDVRKAAKEQVESLGAEFIEVMQE
                     QDSETAGGYATETSEEYKKLQAELIDQYAKIADIVISTALIPGRQAPVLLYKKTVEQM
                     KPGSVIVDLATSRGGNCELSVADKTIKHGEITIVGLSNMAGLVPATASDLYSNNLVHL
                     INLLAKNPAELNLNPDDEIIQQAVLCHKGTYLPFQGVKEKQNA"
     gene            2111..2407
                     /locus_tag="I9760_002597"
     CDS             2111..2407
                     /locus_tag="I9760_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HAT5044346.1"
                     /translation="MPEIHTLDNPYITILTIFVLACFVGYYVVWKVTPALHTPLMSVT
                     NAISSIIILGALIAAGSELIGCITWLGGIAIFITSINIFGGFVVTQRMLRMYKK"
     gene            2431..3828
                     /locus_tag="I9760_002598"
     CDS             2431..3828
                     /locus_tag="I9760_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HAT5044347.1"
                     /translation="MNSIVPLFYLLAAICFILALKGLASPATSRRGNLLGILGMILAV
                     GSTLMMPTTYHHILLIALIAGGGIIGTIIALKINMTAIPQLVAAFHSLVGMAAVLVAY
                     CAFLSPASFQIGTPGAIATASLIEMSLGLIIGAITFSGSVIAFLKLQGLISGIPIRFY
                     AQNYVNLLIGLTILVLLILFVANQNLFLFNLLAGLAFLIGVLLIIPIGGADMPVVISM
                     LNSYSGWAAAGIGFTLSNHLLVITGALVGASGAILSYIMCVGMNRSIFNVIFGGINSS
                     GNTKQAHSANELRNVRQANGEDAAFLLSNAKDVIIVPGYGMAVAHAQHAVKELVDALE
                     SRSIRVRFAIHPVAGRMPGHMNVLLAEANIPYDRVFEQSEINRDFATCDVAYVIGAND
                     ITNPAAKTDPSSPIYGMPVLEVEKAKTVLFVKRSLSPGYAGVENDLFFHDNTYMLFGD
                     AKVMTESIAKALGEI"
     gene            3963..4346
                     /locus_tag="I9760_002599"
     CDS             3963..4346
                     /locus_tag="I9760_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044348.1"
                     /translation="MLRKIGFTLLCAVATFSSSTYSSVTHTLQAGVTLEYEFPPNEPL
                     VFVNYLYWEVAADCKITSEDPSNELFAEALAKAGKINGVTLSKGNTMKVTVHSGDILK
                     LSAESGAKVQITNFGPHTLKATCTA"
     gene            complement(4412..5992)
                     /locus_tag="I9760_002600"
     CDS             complement(4412..5992)
                     /locus_tag="I9760_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAT5044349.1"
                     /translation="MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTV
                     KGRKADRHATSDWMEMEKERGISITTSVMQFIHNQHVINLLDTPGHEDFSEDTYRTLT
                     AVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVL
                     GIQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIG
                     DSADELREEIELVKGASHGFNLEDYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGP
                     QPRATQERVVSPNEETFSGFVFKIQANMDPKHRDRIAFLRVCSGAYKKGMKLSHLRIG
                     KEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAP
                     ELFRLVRLRDPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRL
                     KHEYKVDCIYESVNVTCARWVYSEDDKAMSEFRTKAYDYLALDGGDMLMYLAPTKVNL
                     TMAEERYPKIKFCATREH"
     gene            6202..>6521
                     /locus_tag="I9760_002601"
     CDS             6202..>6521
                     /locus_tag="I9760_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044350.1"
                     /translation="MSTSESELRKSMQQYGKQAFSETQQIEILNLLLEDAKKQLPREH
                     EIYQLIDNFQFRLSHHIHLSEAIQFVNKFIIELESKDKDSSTVNYLKQHGFYRILYRH
                     PKLLG"
BASE COUNT         2035 a         1339 c         1211 g         1936 t
ORIGIN      
        1 tcctttattc ttaataataa attatgagga tttcctatga atctgataaa aattaaatct
       61 aaaaccctat tcatggtgct tgtatacagt ttgctctccc ctgcttatgc agcatcacac
      121 cccaaaagca cagtgaatga caccgccatt caaaatgaaa tattagttta tatcaataat
      181 taccgacaac agcatggcct ggctcctctc aaaatggata accgcatagt aaaagaagca
      241 aaaattcatt ccatggatat ggcaaaacac gcaataccat tcggccataa atattttaaa
      301 aaacgtattg ataaattaca cacacaaatt aaaaactcaa atgcaggagc tgaaaacgtg
      361 gcttacaact ataaaaatgc ccaggatgtt gtcaaaaact ggttacgtag cccaggacac
      421 aaaagaaata tcgttgggaa ctatgacctt accggggtag gtatagctcg agatgaaaag
      481 ggaaaaatat attttactca aatattctta aaaacaggaa atagccacta tgcctccaga
      541 aaacccttcc catcaatttt tagtggtgcc ctcttcagca agaaagcata aaaattaaaa
      601 tagggtgttt aacaaaattt ttgcaatttt taagtacaat tgagtggaat taatgacaac
      661 gttagcctaa tattgaaaca tagacggcaa tcattgttca gccaacagta caattttgga
      721 ggccagattg agagtgtggt gagcaagctc agcctagact gagaagccaa aagcactccg
      781 gaggaatcca cagttttaaa caggactgca ctcaatcttg ctgtctataa catactaaca
      841 ccatggggcg tattcaatac gcccttttta ttggttcatt cctcgcttgt ccggatccaa
      901 agatccaaaa ttggtatttt atgctgtaga aaattattct aaattacaat aggatagaat
      961 aataacggta ataaggattt tcccatgatg attgcaacct taatggaatc agctcaggaa
     1021 acgcgagtag ctattactcc taactcggcc aaacaataca taaaactagg ctatgatgta
     1081 gggattgaaa aaaacgcggg gcttagctca gattttgcta acgaagaatt tgaaaaagct
     1141 ggcgcaataa tattcgatag taaaagtaca ttactcaaaa aaacacaaat tcttttatgt
     1201 gttaacgaac ctgatcccaa aaacctgaat ggcttacctc cagattctct aataattgga
     1261 catatagaca ataacccact tagcccgctt gttaagtggt gtctggatca aaaaatgaca
     1321 ttattttcaa tgaatctcat ccccaggata agtcgtgctc aaagcatgga cagcctatcg
     1381 tctcaggcta acctggctgg ttacagggca gttttggaag ctgcggcaac atttcataga
     1441 gctataccta tgatgatgac agcagcaggc atgatacaac cggccaaagc acttattctt
     1501 ggtgccggag ttgcaggctt gcaagccata gctactgcga aacgtttagg tgcagtggtt
     1561 tatgcctttg acgtaagaaa ggcagccaaa gaacaggttg aaagtttagg cgctgaattc
     1621 atagaggtaa tgcaggagca agatagtgaa acagctgggg gatatgccac agaaacaagc
     1681 gaagaatata aaaaacttca ggctgaactc attgatcaat atgcaaaaat tgcagacatt
     1741 gtaatttcta ctgccttgat accagggcgt caagctccgg ttttacttta taaaaaaact
     1801 gtcgagcaaa tgaaaccagg ttctgtcatt gttgatctcg caacgtctcg aggtggaaat
     1861 tgtgaactca gtgttgccga taaaaccatc aagcatggtg agattactat agtgggatta
     1921 agtaatatgg caggtctcgt ccctgctacg gcaagtgacc tttattctaa taatctggtt
     1981 catcttataa accttttggc gaaaaatccg gctgaactta acttaaatcc tgatgatgag
     2041 attatccagc aagccgttct ttgccataaa ggaacttacc tgccctttca aggagtaaag
     2101 gagaagcaaa atgcctgaaa ttcatacact tgataatcct tatattacaa tattaaccat
     2161 tttcgtactg gcctgttttg taggttatta tgtggtttgg aaagtaacac cggctttaca
     2221 tacaccccta atgtcagtaa ccaatgccat atccagtatt attatacttg gtgctttaat
     2281 tgctgcagga agtgaattga tcggatgcat aacctggtta ggtggcatag ccatattcat
     2341 tacttcaatt aatatttttg gtggctttgt agtaactcaa cgcatgcttc gcatgtataa
     2401 aaaataatga tttaaaggac ggaatcagta atgaattcaa tagttccact gttttattta
     2461 ttggcagcca tttgctttat attggcttta aaaggactgg ccagtccagc tacttcaaga
     2521 agggggaacc ttctaggcat tctgggtatg atcctggcag tgggctccac cctgatgatg
     2581 cccactacct accatcatat tcttttaatc gccttaatag caggcggcgg aataatagga
     2641 actattattg ctttgaagat caacatgacg gccataccac aactggttgc tgcttttcat
     2701 tctttagtag gcatggcagc agttctggta gcctattgtg cctttttatc ccccgcgtcc
     2761 tttcaaatcg gcacacctgg tgctattgct accgcaagtc taattgaaat gagcctggga
     2821 ttaatcatcg gagccattac cttttctggt tcagtcattg cctttttaaa attgcaagga
     2881 ctcatatcag gcattccaat tcgattttat gctcaaaact atgtcaactt actaatagga
     2941 ctaacaatcc tggtgttact cattttattt gtagccaatc aaaacctgtt tctctttaac
     3001 ctgctcgcgg gattagcttt tctcattggg gttttactga ttattcctat tggcggagcc
     3061 gatatgcccg tcgttatttc catgctgaac tcctactcag gttgggccgc agcaggaata
     3121 ggtttcactc tgagtaatca tctgttagtt atcacaggcg ctctggtagg cgccagcggg
     3181 gcaattttaa gctatatcat gtgcgttggt atgaatcgct ctatttttaa tgtcattttt
     3241 ggtggaataa acagttctgg aaatacgaaa caagctcatt ctgctaatga gttacgcaat
     3301 gtcagacaag ccaacggcga agatgctgca ttcctattaa gtaatgccaa ggatgtcatc
     3361 attgttcctg gatatggaat ggcagtggca catgctcaac atgcggtgaa agagcttgtt
     3421 gatgccttgg aatctcgatc tattcgtgtt cgttttgcaa tacaccctgt tgccggacgc
     3481 atgcctggcc atatgaacgt attacttgct gaagcaaata tcccctatga cagagtcttt
     3541 gagcaaagtg aaattaacag ggattttgct acctgtgacg ttgcttacgt gattggagct
     3601 aacgatatca ccaaccctgc agctaaaact gatccttcct cccccattta tggtatgcct
     3661 gtcctggagg ttgaaaaagc taaaacggtc ttattcgtca aacgcagttt atctcctggt
     3721 tatgccggtg tggaaaacga tttatttttt cacgacaaca cctacatgct tttcggtgat
     3781 gccaaagtaa tgactgaatc aatagctaag gctttgggag aaatataacc ctttcaaaat
     3841 tatagcaata atctctgacg gggtgggtac ccggttcaat cgacgcaaat gccttttaat
     3901 ttgccaacga agcaaaataa atgtatctta gccagttaat ttttcataat ggagatatat
     3961 ctatgctacg aaaaatcggc tttacgttgt tatgcgcagt ggcaacattt agttcaagca
     4021 cttattccag tgtgactcac acactccaag ctggagtaac gctggaatac gagtttcctc
     4081 ctaatgaacc tctagtcttt gttaattatt tgtattggga agtagcggcc gattgtaaaa
     4141 ttaccagtga agatccatcc aatgagcttt ttgcagaggc actcgcaaaa gccggtaaaa
     4201 ttaatggtgt tacgctctct aaaggaaata ccatgaaagt gactgtccat tccggcgata
     4261 ttttaaaact aagcgcggaa tctggagcaa aagttcaaat caccaatttt ggaccacata
     4321 ctctcaaagc aacttgtact gcataaagaa ttgagctaac cgcaccccaa cactgaacag
     4381 taatggggtg cgaacattca aatcctgttt atcaatgttc tcgagtagca cagaacttaa
     4441 tcttgggata acgttcttct gccattgtca aattcacttt ggtaggcgcc aggtacataa
     4501 gcatatctcc tccatccaaa gccaaataat cataagcctt ggtacgaaat tcactcattg
     4561 ctttatcatc ttcagaataa acccatcgag cgcaagtcac attgactgat tcataaatac
     4621 aatccacttt atattcatgt ttcagtctat gcgctaccac atcaaattgt aatatcccaa
     4681 cagctcccaa tattaactga ttgctgttta atggcctaaa aacctgggtg gcgccctctt
     4741 cagataattc gattaatcct ttaagcaagg ctttactctt taatgggtcc ctcaacctga
     4801 caagcctaaa caattcagga gcaaaattag gaattccagt aaactttaat tgctcacctt
     4861 gtgtaaaagt atcaccaata cgaatagtac catgattatg caaaccaata atatcaccag
     4921 ctaaagcaag ttctgtgtgt gatctatcgc ctgccataaa agttaaggca ttggaaattt
     4981 gcacctcttt tcctatgcgt aaatgactta atttcattcc cttcttgtaa gcgccagaac
     5041 aaaccctcag aaaggcaatt cggtctctgt gtttgggatc catattggct tgaattttaa
     5101 aaacaaaacc ggaaaatgtt tcttcattcg gagaaacaac tctctcctga gttgctcgag
     5161 gttgaggtcc tggcgcatac tcgacaaaat cgtccaataa ttctttaata ccaaaattat
     5221 taattgctga accaaagtat accggggtca tttttcctgc aagataatct tccaagttaa
     5281 acccgtggga cgctcctttg actaattcaa tttcttctct gagttcgtct gcactgtcac
     5341 ctatcaactc atctaattga ggattatcca atcctttaat ctgcatagct tccacttttt
     5401 ttgcattact gccttgctga tataaataaa taatatcttg atagcggtga taaattccct
     5461 tgaagcgttt acccattcca actggccagg taattggcgc acattgaatg cccaaaacag
     5521 actctacttc atctaacaaa tcaataggtt ctcgcccttc tctgtccaat ttattgataa
     5581 aagtcatgat cggtgtatca cgtaaacggc aaacttccat caattttacc gttcgctcct
     5641 ctactccctt ggccacgtca attaccatta aggcagaatc tacagcagtg agagtacgat
     5701 aggtatcttc agaaaagtct tcatggcctg gtgtatctaa caaattgatg acgtgttgat
     5761 tatgtataaa ctgcataaca gacgtggtaa tcgatattcc tctctctttt tccatttcca
     5821 tccaatcaga agtggcatgt ctgtctgctt tacgaccttt gaccgtacct gccaactgaa
     5881 tagctcctcc aaaaagcaat aacttttcag ttacagtggt ctttcctgca tcagggtgag
     5941 aaataatggc aaaagttcgc cttttattaa agtcttgata aaaatcagac ataggaatct
     6001 cggattaagt ggttaaatgc cttgaattgt aaaggaaaag aacaaaacta acaatttttt
     6061 cgttataaat taaaatctcg ctggtgttta tccatttcat tatagtgttc aggagtgcaa
     6121 ctcaaatttt aaacttaaaa atcaattatt gatcaatttg atttttattg ataaaattgc
     6181 cgtttttttg tggagccgta catgtcaacg tcagaatcag aattaagaaa atcaatgcaa
     6241 caatatggaa aacaggcatt cagtgaaaca caacaaatag agattttgaa tttgttactt
     6301 gaagatgcca aaaaacagct tcccagagaa catgaaatct atcaattaat agacaatttc
     6361 caatttagac taagtcatca cattcattta agtgaggcta ttcaattcgt taataaattt
     6421 attattgaat tagaatcaaa agataaagac agctctacag ttaattattt aaaacaacat
     6481 ggtttttata gaatactata ccgtcatcct aaattattag g
//
LOCUS       DACTJE010000173         6457 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.174, whole genome shotgun sequence.
ACCESSION   DACTJE010000173 DACTJE010000000
VERSION     DACTJE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.174"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..632)
                     /locus_tag="I9760_002602"
     CDS             complement(<1..632)
                     /locus_tag="I9760_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5044351.1"
                     /translation="MLIEHQIKHYRTLYEWFQSPLGVFVAKEFTDQLDPVKKFLKGET
                     LLQLGDCATNPWLNLLDFKHKWIASPFSSTNRIHLECSLNQLPLNRDTLDCVIVPLTL
                     EPFGNNFSLIDEIDRILKPMGFLIFLCINPWSLWGGAMKWGVLNCYGNRTVKLRSAFN
                     LNRIFLQRGYKQCSLTNFCYIPPVNSQSLIKKLTFFDEIGKMIWPFPSGF"
     gene            891..2615
                     /gene="pilB"
                     /locus_tag="I9760_002603"
     CDS             891..2615
                     /gene="pilB"
                     /locus_tag="I9760_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HAT5044352.1"
                     /translation="MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKMSLLQ
                     YIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTIPANLVNEKLIKRHAMVPLFSRG
                     TNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
                     DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYERE
                     YRIRYRQDGILHEVATPPASLSSRITARIKVMSNLDISERRIPQDGGFKMKISKSRAI
                     DFRVSTCPTSAGEKVVMRVLDSGTAKLGIEALGFNPVQRTNFLKAIQRPQGMILVTGP
                     TGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFL
                     RQDPDIIMVGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIA
                     SSVTLIIAQRLARKLCNQCKAVRDDFTNQGLIELGFKEADLVNLKLYKAVGCEQCTSG
                     YRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKEGITTIE
                     EVNRVTVD"
     gene            2621..3841
                     /locus_tag="I9760_002604"
     CDS             2621..3841
                     /locus_tag="I9760_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HAT5044353.1"
                     /translation="MDKNSPPLLTFHYQGINKAGQKMEGDIQARSLAIAKADLRKQGI
                     VTNKVVKKRKPLFDRKNKKITQADITVFSRQLATMIESGIPLVQAFDIVAKGQSNKRL
                     KDLIETIKHDIETGLTLAESLIKHPLYFNELFCNLVDAGEKSGSLDIMLDKVATYKEK
                     IEIIKKKIKKALTYPIAVMVVALLVTAGLLIYVVPQFESLFKGFGADLPAMTRAVITM
                     SEFMQAYWYIIFGALGGIVYSFFYAKNHSLEFAQTIDRVMLKFPVIGPILEKAAIARF
                     ARTLSITFAAGLPLVEALKSVAGATGNIIYAKATDKIREEVATGQQMFTAMENTHLFP
                     NMVIQMVAIGEESGALEKMLSKVADFYEEEVNNAVDALSSLLEPIIMSILGILVGGLV
                     VAMYLPIFKLGSAV"
     gene            3887..4750
                     /locus_tag="I9760_002605"
     CDS             3887..4750
                     /locus_tag="I9760_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAT5044354.1"
                     /translation="MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWK
                     EQCCELFHLEQRKEKIKLNLFLPRSFCPHCKAMVKAWQNIPLLSYILLRGRCYQCDSP
                     ISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
                     GLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAA
                     FGAWLGWMYLPLILLISSITGAIIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGD
                     SIINWYIGYWM"
     gene            complement(4764..5969)
                     /locus_tag="I9760_002606"
     CDS             complement(4764..5969)
                     /locus_tag="I9760_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HAT5044355.1"
                     /translation="MNQNDASKKDNDNSNQLDTELIGSYQSITIGFSKDELASFSKIG
                     QHKHYTAGSIIYNENDLADSFFIIIDGVAEIFKKTIDEFGEHNIHILATLSKDDVVGE
                     MALIENSRRSASVKAKTDLSVIEYPLEVVKAKPKLNLMLIKNMAQILSRRLRFTNEVT
                     VKNMQQALKEASGRNTLGVFIIALLWIICLYTLSLHFLVTVIHQVTVTTVLSICLISI
                     FAVAILIAMHFTGLPYSRFGITAKDWGKTILEALAFTIPIMLFVLIIKVFFIIGIDHL
                     DNTPIFSGTKRFLVNNEVDWEYYFAMIFLYSLFAPIQELMARSALQSTFFHFLPGEKL
                     FRQWNGIFLSNLIFATMHTHLGLTFASLTFIAGLFWGWLFHRQKSLLGVSVSHVILGV
                     WSLFIVGLR"
     gene            complement(6077..>6457)
                     /locus_tag="I9760_002607"
     CDS             complement(6077..>6457)
                     /locus_tag="I9760_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044356.1"
                     /translation="NLDDSAANFQLGQIFLDEAKYRQKLDNEGIFNSQANLKRAQQLF
                     DEAHAHLIAAEKLGHVGAKRLRGLCIINGWGVESDKNAGFELVVDSIEQEGSWDKIPQ
                     IFASMGLNKPEFFSAIMQRRKGAS"
BASE COUNT         2088 a         1099 c         1228 g         2042 t
ORIGIN      
        1 taaaagccag aaggaaaagg ccatatcatt ttgccaattt catcaaaaaa agttaacttt
       61 ttaattaaag attggctatt tactggcggt atatagcaaa aattagttaa agaacactgt
      121 ttgtagcctc tttgaaggaa aattctgtta agattgaaag ctgaacgcaa ttttaccgtt
      181 cgattaccat aacaattaag caccccccat ttcatagccc ctccccataa actccaagga
      241 ttaatgcata agaatatgag aaatcccata ggcttcaata ttcggtcaat ttcatcaatt
      301 aagctaaaat tgtttccaaa cggttctagt gttaaaggta ctataacaca atccaaagta
      361 tctctattca gtggtaactg atttagcgag cactcaagat ggattctatt tgtagaagag
      421 aatggtgatg ctatccattt gtgtttaaaa tcaagaaggt ttaaccaggg atttgtagca
      481 caatctccaa gttgtagtaa cgtctctcct tttaagaact tttttactgg atctaattga
      541 tccgtaaatt ctttagcaac aaatactcct aaaggtgatt ggaaccattc gtataaagtg
      601 cgataatgtt ttatttgatg ttcaatcaac aaaatgtgac ctttaattta ttacctttta
      661 ttaactacaa tacaacgagt tttagatgca aacaatttaa atctttcaaa ttttttatta
      721 tttccaagag agttgcgtta tagcccgtgt ttgcaaaaaa ttgatatggc aaagtaacca
      781 gaaataggat ttaggggcag gtccaaaaca ccgggttttg ttcaaaaaac catgcaatta
      841 caaaaaaatt taactatatt taaaactaag atacactttg aataaatgaa atggctctag
      901 ctacagaaga atatagatta cagggaatcg gccagcttct cgtgctggaa aagctgctag
      961 ataaaacaaa agcaattgaa ctacacaagt tggcagcagc agaaaagatg tctttgctgc
     1021 aatacattgt aaaaaataaa atattatctg ctgaacaaat tgcactgacg gctgctcaaa
     1081 attttggtgt acccatgttg gatattaatt gcatcgatgt gggcaccatt cctgccaatc
     1141 tggttaatga gaaattaatt aaacgtcacg ccatggttcc tctttttagt cgaggcacta
     1201 atttatatct tgcaacagat gatcctagta aacaagcttc attaaaggaa attcaatttc
     1261 ataccggatt aaatactcat gcgatagttg tagaaacaga taaacttagt gctctgattg
     1321 ataacctatt aacagcaaag gaaagtcagg gcttatcaga gtatgttgaa gactctggag
     1381 atctggaagg tttagaaata agtgctgatg atgaagatca agatattgat actgcaacat
     1441 cagtaactga tgatgcccct atcgtgaaat tcgtcaataa aattttattg gatgcaataa
     1501 ggcaaggagc ttctgatata cactttgagc cttatgaacg agaataccgc atcaggtaca
     1561 ggcaagatgg tattttacat gaggttgcta cccctcccgc aagcttgtca tctcgtatta
     1621 cagcacgtat caaggtaatg tctaatttgg atatatcaga acggcgtatc ccccaggatg
     1681 gtggcttcaa aatgaaaatt tccaagtcac gagcaattga tttcagagtg agtacatgtc
     1741 caacctcggc tggggaaaaa gtggttatgc gggttcttga ttctggtact gctaaattag
     1801 gtattgaagc attaggattt aatcctgttc agcgaacaaa tttccttaaa gcaatccagc
     1861 gcccacaagg gatgatttta gtcactggcc ctactggtag tggtaaaaca gtcactttat
     1921 atacggcttt aaatatatta aatacaattg aggttaatat ttcaacagct gaagatccag
     1981 ttgaaatcaa agtacctggt atcaatcagg ttaatattaa tcctaaagct ggcctaacct
     2041 tttccggtgc attgcgttcg tttctaaggc aagatcctga tattataatg gttggtgaga
     2101 tacgagacct tgaaacagca gagattgctg taaaagctgc tcagacaggg catttggttc
     2161 tttctactct gcacacaaac agtgccgctg aaactttaaa tcgtttagta aacatgggaa
     2221 taccaacttt taacatcgcg agttcggtta ccttaattat tgcgcaaagg ctggcaagaa
     2281 aattatgcaa tcaatgtaag gcagtgcgag atgactttac caatcaaggt ttgattgaac
     2341 taggtttcaa ggaagctgat ctggttaatc ttaaattata caaagcagtt ggttgcgagc
     2401 aatgtacaag tggttatcgc gggcgagtcg ggttgtttga ggttttaccg atgacgaaag
     2461 agcttggcca attgattatg tcaggtggta attcgctgga tatattaaag ttagctcaat
     2521 cagaaggtat gctgactatt tttcaatcgg gtatagaaaa agttaaggaa ggaatcacga
     2581 ccatagagga ggtcaatcgg gtaaccgttg attaatttgg atggataaaa atagcccacc
     2641 cctattaacc tttcattatc aaggcattaa taaagccggt caaaaaatgg agggtgatat
     2701 tcaagcgaga agcttggcaa tagctaaagc tgatttgcgt aagcagggga ttgttaccaa
     2761 taaagtagta aaaaaaagaa aacctttgtt tgatagaaaa aataaaaaaa tcacacaagc
     2821 agatattact gtgtttagcc gccaattagc gacgatgatc gagtcaggaa taccattagt
     2881 gcaagcattt gatattgttg ccaaaggaca aagcaataaa agacttaaag acttaataga
     2941 aacaattaag cacgatatcg aaacggggct aacgctggca gaatccttaa ttaaacatcc
     3001 actctatttt aatgaattat tttgtaatct tgtcgatgct ggtgaaaaat caggctcatt
     3061 agatatcatg cttgacaagg ttgcaacgta taaagaaaaa attgaaatca taaaaaagaa
     3121 aataaaaaaa gctctcactt acccaattgc tgttatggtt gtcgcacttt tggttactgc
     3181 gggattatta atctatgtgg ttcctcaatt tgaatcgttg tttaaaggat ttggagctga
     3241 tttgccagcg atgactagag ctgtgatcac tatgtctgaa tttatgcagg cttattggta
     3301 tattattttt ggagctttgg gtgggattgt ctatagcttt ttttatgcta aaaatcattc
     3361 tttagagttt gcgcaaacca ttgacagagt gatgctaaaa ttccctgtaa ttggccctat
     3421 tttagagaaa gcagcaattg ctagatttgc gaggacatta tccattactt ttgctgctgg
     3481 attacccctg gttgaagcat tgaaatctgt agctggagcg accgggaata ttatttatgc
     3541 caaggcgact gataaaatca gagaagaagt tgcaacagga cagcaaatgt ttactgctat
     3601 ggaaaatact catttatttc caaatatggt aattcaaatg gtagcaattg gtgaggaatc
     3661 aggtgccctt gaaaaaatgt taagcaaagt ggctgatttt tatgaggaag aagtaaataa
     3721 tgctgtagat gccctgagta gtttattgga gccgataatt atgtcaatat tgggtatact
     3781 ggttggggga ttggtggtgg ctatgtattt acctattttc aaactgggtt ctgctgttta
     3841 aacagatcct atttaattta atcatacaat ataggcaaac gtgtcaatga taaatgcact
     3901 aataatcaat tatccatggt ttatgtattt agtcgttgga ctcttttctc tagccgttgg
     3961 aagtttgctt aatgtgataa tctatcgctt accaattatt ttgcaggaag agtggaaaga
     4021 gcaatgctgt gagctattcc atcttgaaca gcgaaaagaa aaaataaaac ttaatttatt
     4081 tttaccacgt tctttttgtc ctcattgtaa agcgatggtt aaagcgtggc agaatattcc
     4141 tctgttaagc tatattttgt taagaggacg ctgttaccaa tgtgatagtc caatttcaat
     4201 ccgctaccct tttgttgaga cgttgaccac agttttatcc ttatacgcca gttggcattt
     4261 tgggtttaca atacaattat tatttgcttt attagctatc tggatattaa tatcacttgt
     4321 ttttattgat cttgatcatc aattactccc tgatagcctc actttagggc tcttatggat
     4381 tggccttatt gctaataccc agaatgtatt tgttagtctt gacgttgctg tattaagctg
     4441 cgctggcgct tatttagcct tatggctatt cattaatctg ttttatctga tgacaggtaa
     4501 agtaggtatg ggacatggag atttcaaatt atttgcagca tttggtgcct ggcttggatg
     4561 gatgtattta ccattaattc tactaatatc ttcaataacg ggggctatca ttggtttgat
     4621 ttatttaaag ataaatggta aatcaagaga tactgcaata ccatttggcc cttttctttg
     4681 catttctgga ttaatagcta tgttttgggg agactcaatc atcaattggt atataggtta
     4741 ttggatgtaa aatgttgatt agcttagcgc aacccaacaa taaatagaga ccacacgcct
     4801 aaaatcacat gagaaacaga tactcccaaa agagatttct gtctgtgaaa taaccatccc
     4861 caaaaaagtc cggcgataaa agttaatgag gcaaaggtta gccccaagtg agtgtgcata
     4921 gttgcaaaga ttagattgga cagaaaaatt ccattccatt gacgaaataa tttttccccg
     4981 ggtaaaaaat gaaaaaaagt agattgtaaa gcacttctgg ccattagttc ttgaatgggg
     5041 gcaaaaaggc tatataaaaa aatcatagca aagtaatatt cccaatccac ttcattattt
     5101 accaaaaagc gttttgttcc tgaaaatata ggagtattgt ccagatgatc gatacctatt
     5161 ataaaaaaaa ccttgataat tagtacaaaa agcataatag gaattgtaaa agctaatgct
     5221 tccaagatag tttttcccca atctttcgca gtgataccaa atctggaata tgggagacca
     5281 gtaaagtgca ttgcaatgag aattgctaca gcaaagattg agattaaaca gattgacagt
     5341 acggtagtga cagttacctg atgtattaca gtaaccagga aatgtagaga taaagtatag
     5401 aggcaaataa tccatagcag agctatgata aataccccca atgtattgcg ccctgatgct
     5461 tctttgagag cttgctgcat atttttcaca gttacttcat ttgtaaaacg aagtctcctg
     5521 gaaagtattt gagccatatt tttaattaac atcaagttaa gctttggttt agcctttacc
     5581 acttctaacg gatattctat aacactaaga tcagttttag cctttaccga cgcagaacgt
     5641 cgactatttt ctatgagagc catttctcct acaacatcat cttttgataa ggtagcaaga
     5701 atatggatat tatgctcacc aaactcatcg atagtttttt taaatatttc ggcaacacca
     5761 tcgattataa taaagaatga gtcagcgaga tcattttcat tataaataat gcttccagca
     5821 gtatagtgct tatgctgacc aatttttgaa aaactggcaa gttcatcttt agagaaacca
     5881 attgttattg attggtaact tcctattaat tcagtatcca attgattgga gttgtcatta
     5941 tcttttttag aagcatcatt ttggttcatt gctattccaa tcctaaatat gtaagtccac
     6001 atttactcta tttaatgcaa cgataatata tacctcataa ataatttttg attaggaaat
     6061 taatcatgaa taaattttaa gatgctcctt ttcttctttg cataatagct gagaaaaatt
     6121 ctggtttatt taatcccatt gacgcaaata tttgaggtat tttatcccaa cttccctctt
     6181 gttcaatact gtccacaacc aactcgaatc cagcattctt atcgctttca acaccccagc
     6241 cgttgataat acatagtcct cttaatctct tggcacctac atgccccagt ttttcagctg
     6301 cgattaaatg tgcatgggcc tcatcaaaaa gttgctgtgc tctttttaag ttggcttgac
     6361 tattaaatat tccttcatta tccagttttt gcctgtattt tgcttcgtcc agaaaaattt
     6421 ggcctagctg aaaattagct gccgagtcat cgagatt
//
LOCUS       DACTJE010000174         6433 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.175, whole genome shotgun sequence.
ACCESSION   DACTJE010000174 DACTJE010000000
VERSION     DACTJE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.175"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            58..243
                     /locus_tag="I9760_002608"
     CDS             58..243
                     /locus_tag="I9760_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044357.1"
                     /translation="MNTDDINKAYVSPYDKFLYEFDATHKKSASQLHEIKKHERIFKM
                     RDDKDYKIDQSEIWEGF"
     gene            342..788
                     /locus_tag="I9760_002609"
     CDS             342..788
                     /locus_tag="I9760_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044358.1"
                     /translation="MKNPSILQLNNPALLNKAMQLKSTGKVAISQNQLTYLKIHDEWI
                     HELFPLLQNEQIRKPDYFGNESIGAHISIIYPEENTTINPKELDQEHRFKIKNIIKAN
                     LELKKYYAIIVEASSLVRLRKAYGLPENLYFKGYSIHFHITVGVEY"
     gene            complement(829..2082)
                     /locus_tag="I9760_002610"
     CDS             complement(829..2082)
                     /locus_tag="I9760_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HAT5044359.1"
                     /translation="MDYSIYLLFAALILCFLMTWGVGANDLANVMSTTMGSKAVTVRQ
                     AMLIAIIFEFAGAFLGGEGVTETMRDGIINTSQLSNEPLILIEGMLCVLFACTIWMNL
                     ASYLGVPVSITNALVGSMVGFGTIVLGPDAIHWNQVARIAIGWVSSPLISGITAYALF
                     ISIQQTIFVKSNPLTKAKLYIPIYLFLIGFILSFITVFKGLNHFDIHLNLKQDLAVTL
                     ATSIIITILGMIFIKRIPEYHKIRRRERFIQVEKYFAVLMAMTACAMAFAHGSNDVAL
                     AVGPLSIVHSLVMHSNQIFDADNYPSWIILLGCVGVVTGFLMYGRKVIETVGSSITAL
                     TPSRAFAATLSAATTVVVATSTGIPVSATQTLVGAVLGVGLARGIGALNLIVIRNIFM
                     SWVLTLPAASMLTILSYKLLHALLG"
     gene            complement(2092..2763)
                     /locus_tag="I9760_002611"
     CDS             complement(2092..2763)
                     /locus_tag="I9760_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HAT5044360.1"
                     /translation="MGSIFNMFGPSPIKPIEQHIRKAHQCAKQLYPFFEAVLKNDWDT
                     ANKIKDKIIAIEKEADLIKRDLRLHLPTGLFLPVARTDILELLSAQDRIANKAEDIAA
                     LIISRQMSIPKKLIPSFMPFLSRCLDASKQACTAINELDELLETGFRGSEVKIVEEMI
                     VKLDEIEHDCDERLADIRHKIFELEKELPAIDVIFLYKLVQWIGELADHAQTVGGRLQ
                     ILIAR"
     gene            2954..3718
                     /locus_tag="I9760_002612"
     CDS             2954..3718
                     /locus_tag="I9760_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/III secretion system protein"
                     /protein_id="HAT5044361.1"
                     /translation="MIKRWLLVILLISGNAFSQQMITKVIELNYLQADKVIQLIQPLL
                     QTDEKVSGSGQTLIVNVSPQTLTQIRNVLHKIDVPPVVFNITVYQGDPEWLSSQDGND
                     VSYSTQPQYEVQRSQSVKVMSGESAFVATGEQVPVVTSVGVGLLATGVSYEQHNIQNG
                     LLVHPVLQGSQVKLTVRRVREQENVESDQQFDNQQIDTTIMAPLNKWVPLGTAEGVQN
                     TDSSSTSYTAGRPFSQNSTLYIKVSVVKSTPSGTTK"
     gene            4020..4583
                     /locus_tag="I9760_002613"
     CDS             4020..4583
                     /locus_tag="I9760_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAT5044362.1"
                     /translation="MAIISSLANHLLIAMPSLKDPNFERSVVYLCEHNEQGSVGLIIN
                     RPLQFPLSIVFEQLQIEPIRVEKNGLPLLFGGPVQPERGFVIHKQMGGWRSSLFLQDE
                     VTVTTSNDIIRAIAYDEGPKDVLITLGYAAWTEQQLEREIMSNTWLVCPYKSEILYEV
                     PFEERWEYAGLTLGIKMNQLSSDAGHA"
     gene            4576..4995
                     /gene="ruvX"
                     /locus_tag="I9760_002614"
     CDS             4576..4995
                     /gene="ruvX"
                     /locus_tag="I9760_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAT5044363.1"
                     /translation="MPRGVYLGFDFGYKRIGVAVGQRLTCSASPLSTIEAKAGIPDWN
                     TIQKVITQWNPQALIVGLPTCIDDRELYTTSAARHFAKQLHKRFSLPVHLVDERLSTV
                     EARGYLFEQGGYRQIKKAEVDSIAACVILEQWLQQSE"
     gene            5045..5938
                     /locus_tag="I9760_002615"
     CDS             5045..5938
                     /locus_tag="I9760_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAT5044364.1"
                     /translation="MKHFLEISQLSSEQIESLLQRALYFKHTKQYPSYSQSIIANLFY
                     ENSTRTRISFELAERHLAMSVVNLDLQTSSEIKGEAIEDTIRTLAAMGIQYFVIRHKQ
                     DGLQQNLANKLGDTVHIINAGDGTHAHPSQAILDMVTIIEQKKRLDKLKIAILGNIKH
                     SRVANSFQCICSKLGVGDLVLISPEIWQPSQVHFGRVTDNLNEGLEGADVVICLRVQR
                     ERLLEDDHLDLDFYRNNFALTQKSLSYAKPDAMVMHPGPMNRGVEIDSEVADGNQSCI
                     LQQVTNGVYARMAILESLIAS"
     gene            complement(6052..6378)
                     /locus_tag="I9760_002616"
     CDS             complement(6052..6378)
                     /locus_tag="I9760_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044365.1"
                     /translation="MAKQIMDNAIMIVKNCYDKSTDLSKTFVPCINEEIEKLPDSKNF
                     QIRMKLKSAEKDNNEKISILMVDKTGYMYYCIVTTGKTLIINACAGEQGKPLTEGQML
                     SIDLPE"
BASE COUNT         2060 a         1150 c         1274 g         1949 t
ORIGIN      
        1 acttagataa aaaaacgata aaatatattt tttaaataaa gttattttgg attgagtatg
       61 aacacagatg atataaataa agcttatgtc agtccttacg ataagttttt atatgaattt
      121 gatgcgacac ataaaaagtc agcgtcgcag ttacatgaaa ttaaaaaaca tgagcgcatc
      181 ttcaaaatga gagacgataa agactacaag atcgatcaaa gtgaaatttg ggaagggttt
      241 taagagtttc tcctctatag ctatcttggt aagctgctag catgttaatc aagacctagt
      301 agcagaaatg acaggtcttt attgtattag taaagataaa gatgaaaaat ccatccattc
      361 tgcaactaaa taatcctgct ttattaaata aggctatgca gcttaaatca actgggaaag
      421 tagccatttc tcaaaatcaa ttaacttatc ttaagattca tgatgaatgg atccacgaac
      481 tatttccgct cttacaaaac gagcagatcc gtaaacctga ttattttggt aatgaatcca
      541 tcggtgctca tattagtatt atctatccgg aagaaaatac aacaattaat ccaaaagagt
      601 tagatcaaga gcatcgcttt aaaattaaga atattattaa agcaaattta gaattaaaaa
      661 aatattatgc cattatagtg gaggcatcct ctttggtaag attgagaaaa gcatatggtt
      721 tgcctgaaaa tttatatttt aaaggatatt caattcattt tcatattaca gtaggcgttg
      781 aatattaggc aatccattgc ctatgatgag cctgataggc aatgaggatt atcccaacaa
      841 agcatgtaaa agtttatagg ataagatcgt tagcatggag gctgcaggta atgtcagtac
      901 ccatgacata aagatattac ggattacaat gagattcaaa gcgccgatac ctcttgccaa
      961 acctacacct aaaacggcac caaccagagt ttgagtcgcc gaaacaggga ttccggtgct
     1021 ggttgcaaca actacggtag ttgctgcaga aagtgttgca gcaaatgcac ggctgggagt
     1081 taaagcagtg atagagcttc ctacggtttc aatgactttt cttccataca taaggaaacc
     1141 agttactact ccgacgcagc ctaataaaat aatccaggaa gggtaattat ctgcgtcaaa
     1201 tatttgattg gaatgcataa ccagactgtg cacaatactt aatggtccaa ctgccagggc
     1261 aacatcgttg gagccatgag cgaaagccat agcgcatgct gtcattgcca tgagcacggc
     1321 aaaatacttc tctacctgaa taaatcgttc cctgcgtctt attttatggt attcaggaat
     1381 acgcttaata aagatcatac ccagaatagt aattattata ctggtggcca gagtcaccgc
     1441 caaatcttgc tttaaattca agtgaatatc aaaatggtta agtcctttaa atacagtgat
     1501 aaaagataat ataaaaccaa tcaaaaaaag ataaatagga atatatagct tggctttagt
     1561 caacgggtta ctttttacaa atatggtttg ctgtatacta atgaacaaag cgtaggccgt
     1621 aatccctgaa atcaacggag atgaaaccca tccaatagcg attcgcgcta cctgattcca
     1681 atgtattgcg tcaggaccca agactatagt gccaaaacca accatggagc ctactaatgc
     1741 gttggttatc gaaacaggga cccccagata actcgcaaga ttcatccata ttgtgcaggc
     1801 aaacaaaacg cacagcatgc cttcgatgag gattaaaggt tcattagaaa gttgactggt
     1861 attgataatc ccatcacgca tggtttcagt gacaccttca ccccctaaaa aagcacctgc
     1921 aaactcgaaa ataatggcga taagcatggc ttgacggacg gtaactgcct ttgagcccat
     1981 agtggtactc atgacattag ccaaatcatt ggcgccgacc ccccaggtca taagaaaaca
     2041 aagaataaga gcagcaaaaa gtaggtaaat tgaataatcc atgagtgata actaccgggc
     2101 aattagaatt tgaagtcgac caccaactgt ctgggcatgg tcagccaatt cgccaatcca
     2161 ttggaccagt ttgtatagaa aaattacatc tatagctggg agttcctttt ctaattcgaa
     2221 gattttgtgc ctaatgtctg ccagtctttc atcacagtca tgttcaattt cgtctaattt
     2281 tactatcatt tcttcaacga ttttgacttc gctgcctctg aatccggttt caagcaattc
     2341 atctaattca ttaatcgctg tgcaggcttg tttagaggca tcaagacatc ggcttaaaaa
     2401 aggcataaaa gacgggatta attttttagg tatgctcatt tgtctgctta taattaaagc
     2461 agctatatct tctgctttat tggcaattct atcttgagca ctgagcagtt ctagtatgtc
     2521 tgtgcgggcg actggtaaaa ataagccagt aggtagatga aggcgcaaat cacgtttaat
     2581 tagatccgct tctttttcaa tagcgatgat tttatccttg atcttgtttg cagtatccca
     2641 atcatttttg agcaccgctt caaaaaaagg gtacaattgc ttggcacact gatgtgcttt
     2701 acgtatgtgt tgttcaatgg gctttattgg tgatggcccg aacatattga atatactacc
     2761 cataattttt gatctcggat aacagatttt aaggattata cctaaacttt ttgaggaaca
     2821 aaactactac ttatctacag tgaatacaga ttagagatta aagggggcta tttgtaacct
     2881 tgtgagtcca actattagtg ctacaattca ataaaggatt ttataattaa attgaaacaa
     2941 caggagtgtt tctatgatta agcgatggct attagtgata ttattaattt ctggcaacgc
     3001 tttttcccaa cagatgataa ccaaggttat cgaattgaat tatttgcaag cagataaggt
     3061 cattcagtta atccaaccat tattgcagac tgatgagaaa gtaagtggtt cagggcaaac
     3121 actaattgtt aatgtgagtc cccaaacttt aacgcaaatc agaaacgtat tacataaaat
     3181 agatgttccg cctgttgttt ttaatattac tgtttatcaa ggggatcctg aatggttaag
     3241 ctcacaggat gggaatgatg tctcttacag cacacaacca caatatgaag tacagcgcag
     3301 tcaatccgtt aaagtcatga gtggggaatc cgcttttgtt gcaaccggtg aacaagtgcc
     3361 agtagttact tcagttggtg ttggtctttt agccacagga gtatcttatg agcagcataa
     3421 tatccaaaat ggtttgctag tacatcctgt tttgcaagga tcacaggtca aattgacagt
     3481 gagaagagta cgagagcagg aaaatgtaga gagtgatcaa caatttgata atcaacaaat
     3541 agatactacg atcatggcgc cattgaataa atgggttcca ttaggtacag cagagggggt
     3601 tcaaaatacc gattcttctt caacttctta tacagctgga aggccttttt ctcaaaattc
     3661 aactttgtac attaaggtat cagttgtaaa atccacccct tctgggacca ccaaatgaaa
     3721 tggtggaaat gcgtttcata atcattttac ttggatcttc tcttaatgag cttttcttcc
     3781 ctgaactgga gaataagcca ggatttactg attaactaac tcctaatccc ttcattaaaa
     3841 atattttgcc ttgatcctgg agtttctcgt aagcatcttt aagccatgaa aatcataata
     3901 attcggtatt gtgggctcat gccaccacaa atagattaaa ttaaaaacaa aacgataaaa
     3961 ttaaaatcat taatggtgta taattaaagt gagcggagag aattttgggg ggacttctta
     4021 tggcaattat cagttcactg gccaatcacc tattgattgc catgccttcc ttaaaagatc
     4081 caaactttga aaggtcagtg gtctatttgt gcgagcataa tgagcagggg tcagttggtt
     4141 taattattaa cagaccttta cagtttccac tatctatcgt ttttgagcaa ttgcaaattg
     4201 aacctattcg agtagaaaaa aatgggttgc ctttattgtt tggtggaccc gtacaaccag
     4261 aaagaggatt tgttattcat aaacaaatgg gcgggtggcg ttcgagtttg tttttgcaag
     4321 atgaagtgac ggttactacc tcaaatgata tcattcgtgc catagcttat gacgaaggac
     4381 ctaaagatgt attaatcact ttaggttatg ctgcttggac agagcagcaa ttggaaagag
     4441 aaattatgag taacacgtgg ttagtttgtc cctataaatc tgaaattctt tatgaagtgc
     4501 cctttgaaga gcgttgggaa tacgcgggat taacgctggg catcaaaatg aatcaactct
     4561 cttctgatgc tggccatgcc tagaggtgtg tatttaggtt ttgatttcgg gtataaacgc
     4621 ataggagtcg ctgtaggaca gcggctaact tgtagtgcct ctcccttatc tactatagaa
     4681 gctaaagctg gaatccctga ttggaatacc attcaaaaag tgataaccca atggaatcca
     4741 caagctttaa ttgtaggtct tcctacctgt attgatgacc gtgaattgta tacaacatca
     4801 gctgctcgtc attttgcaaa gcaattgcac aagcggtttt ctttacctgt gcatttagtc
     4861 gatgaacgac tttcaacagt agaggcaaga gggtatttgt ttgagcaggg gggatatcgc
     4921 cagattaaaa aagccgaggt agatagtata gcagcttgtg ttatactgga gcagtggctg
     4981 caacaatcgg aataactcaa agagtacttt tcttttgatg caaaatgaaa tttggcgggg
     5041 gaacatgaaa cattttcttg aaattagtca attgtcatct gaacaaattg aatccttatt
     5101 gcaacgagct ttatatttta aacatacaaa gcaataccct tcttattccc aaagtattat
     5161 tgctaattta ttttatgaaa acagtacccg aactcgcatc agctttgagt tagcagagcg
     5221 ccatctggca atgtctgtag taaatcttga tcttcagacc tcatcagaaa ttaaaggcga
     5281 agccattgaa gataccatca gaacgctagc agctatgggt atccagtatt ttgtaattcg
     5341 ccataagcaa gatgggctgc agcaaaattt agctaataag ttaggtgata cagttcatat
     5401 aataaatgct ggtgatggaa ctcatgctca tccaagccaa gcaatactgg atatggtgac
     5461 aattattgaa caaaaaaagc gattagataa attaaaaatt gctattttgg gaaatattaa
     5521 acattcacgt gttgctaatt ctttccaatg catttgcagt aaactagggg ttggtgactt
     5581 ggttttaatt tctccagaga tttggcaacc atcacaagtg cactttggac gggttactga
     5641 taatctcaat gaaggtctgg aaggtgcgga tgtggtaatt tgcctgcgag ttcaaaggga
     5701 acgcttgctg gaagatgatc atttggattt ggatttttat agaaacaatt ttgcacttac
     5761 tcaaaaaagt ctgtcttatg caaaacctga cgctatggtt atgcaccctg ggcctatgaa
     5821 tcgcggggtt gaaattgata gtgaagtggc tgatggcaac caatcttgta ttttacaaca
     5881 ggttactaac ggtgtttatg caagaatggc tatccttgaa tcgctgatag cgagttagat
     5941 ttctctatat tctgtcatgc attatgcttt tattctgttg ggtaatatat tctactcata
     6001 ctgcaagacg catgattaac cacgctgagc aagagatagg aaaccgcaaa attattcagg
     6061 tagatcgatg gataacattt gaccttccgt caatggtttt ccctgttctc cggcacaggc
     6121 atttatgatt aaagtttttc ctgtggttac tatgcagtaa tacatatatc ctgttttatc
     6181 gaccatgaga atagatattt tttcattatt atctttttca gcacttttca atttcatgcg
     6241 tatttggaaa tttttagaat cgggtaattt ttctatttct tcattaatac atggtacaaa
     6301 agttttgcta agatcagtgg atttatcata acaatttttt actatcataa tggcattatc
     6361 cataatttgt ttcgccatta aaagtttgca agggaataca atacaacaga gggtaagcat
     6421 taagcgtaat atc
//
LOCUS       DACTJE010000175         6213 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.176, whole genome shotgun sequence.
ACCESSION   DACTJE010000175 DACTJE010000000
VERSION     DACTJE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.176"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1098)
                     /locus_tag="I9760_002617"
     CDS             complement(<1..1098)
                     /locus_tag="I9760_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAT5044366.1"
                     /translation="MSPRICCYNQYPGLYQDFDAMREDLQRIQKMGFKQVWVNPFYTP
                     CQYNPVPNMANRIHSPYAMQDESIYPRYAKNDKSVQQYTAKANELGLMPIFDLVARHL
                     AVDHRFVNGDKFFLEKYNIDTKKWFKRHPNGNLVMHNMDENYNPLANNPWSDVATFNY
                     DDPVICEQIIEYYWKPFIERNIEHLGFQGIRIDAPAMVNNKVLSRLTEIAKEACQHKF
                     QRDPLIIAETIGRGYMEENLALKGIVTHTMNSVFWMPGPEGGYGYSYNLWQQDDNWFT
                     QNKGLLQQVGPTIGFPGSHDEPRYIQQLVEKGIHDDKLLAKRMREKLAVSAFCSDGGW
                     ILQYGDEYGATKPVNVFDPTPVEYHQQNHLRR"
     gene            1265..2938
                     /locus_tag="I9760_002618"
     CDS             1265..2938
                     /locus_tag="I9760_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2527 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044367.1"
                     /translation="MLYFVLCDLKFNQFSRYPMSYQKIELSISLSLDSPKLDESDFIL
                     LSVKYLEKSLGKKKEFSGFFEDIEQLYFKQNYKEAIEKILDFSKKNESLLSEQVVQRL
                     AEVAPRLKSNPKDNESRRLYETLYADHLESVIKQESDLSVFNELRDSYNAVKPEYAVT
                     HETEIKTLDEAKQFILSFVMLNDNVELPLKAQSERYPKKDRSREELGNTPSANPGIMK
                     PNSPNFTDNLVPVRDVPKIAINEKVAGGYSKTKPTTPFVASLSGTTYSLMVVLTDYIE
                     KHKTDKDIEKKVNQIINLWISSYIKEGYHSYSEVVDVLTEPFLQSIFDKANIKLNYGV
                     LEDTHAEFRKAQDYVFGLTIRSAMHHELQERFKNKEKLQEEVKNFESGLNKLNQNVEN
                     TGRHREALNKLNEVFQDWSAGKKSYDAFKGESNQLIYDIESEEQKSNRGLGSMLKNLG
                     NYLLYLITLRFLKEDYPKPSSPVTMLVTELRGTLEQIDHFHKTSLSLKKATQPDPKNT
                     SPSGETTDVLKSAVDTDKHDKNQESEVIKTLDSEQLEEERQDDKIARLQ"
     gene            3283..4650
                     /gene="mavL"
                     /locus_tag="I9760_002619"
     CDS             3283..4650
                     /gene="mavL"
                     /locus_tag="I9760_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavL"
                     /protein_id="HAT5044368.1"
                     /translation="MRLFQYNQHICRGRFPVSNAYCKDFVVRNINTIFGLSENIMAYQ
                     LLLSKETLNKILQYKQNLEKGLATPGKFFLEELSKQEKSISEMDITTFTQLLIQSKKP
                     QVFAESQVYHDGTDWTLEEESILGDVSVNMPVTMYNDGGHGSSFKNHPEPISGYLAYV
                     PGALLASGSGPTSDMKEVLDNGKLNQDKLNALYERRLLPQLIHFNELARQNEKQAAIT
                     IPGIGTGCFSGAYYDVIKPYVRNALIHILEKHKDSLPYIDIIHYDPYMGDEPAEKKIG
                     HMSFRVSPSGVVRGTTGQLDYPLGSNPDTHILVSIVAWDHFSWPGNDYWGGARQTDDG
                     VKAASTDTMGQVTGATGVYDKKWGRYMPPESFTKDRKGMSDWGDYVRENGIVFNGPVL
                     ALDKSGKLDTLENVASRSSKAKVETTTTISDLVRSMFSLFSHSTTTEPSPTVKEEESK
                     KSGPQ"
     gene            complement(4931..>6213)
                     /locus_tag="I9760_002620"
     CDS             complement(4931..>6213)
                     /locus_tag="I9760_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lpg2525 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044369.1"
                     /translation="IFADKNLDIQNIIVVSMVCKLWYELSLDNAVWRPLFIKRFSYSP
                     TLANHSIFKEDYQKQLALTQAMKKKCESNHACRNEPGLLEAIREKNIPLIYALFESGH
                     RIIIDSFPDGEQQFTEEMLGFLSECAELPSSLLARIFKRGFCTADMETSEFLALHFIV
                     AEADEKLLQKIFRALGDDLLSVSRYCDLYDNNFFSSLLHFPVERFSLVLRLLYETLGK
                     TKLSTLLVYQNHTYTDWSCIHSASRCGREYFFITLKYLKRQDYPVLLKKGLNDEDIPL
                     FNIAYWGDKESLSHIINILGADATTFLKYKDNKGRNILLPALLSEDHSTVELIEKVMG
                     MEFIDLLLMQDTNGRDILRRVMLLQEPTVLRRINTVLKLLRDYDCLDIAQELYSSDEF
                     TQLISQNLYINQDETIELFNKYTEEKTNNFCSIL"
BASE COUNT         2006 a         1101 c         1170 g         1936 t
ORIGIN      
        1 tcgtctcaga tggttttgtt gatgatattc aacaggggtt ggatcaaaaa cgtttactgg
       61 cttggtagca ccatattcat caccgtattg taaaatccaa ccgccatcag aacaaaatgc
      121 gctgacagcc agtttttcgc gcatgcgttt tgcaagcaat ttatcatcat ggataccttt
      181 ttccaccagt tgctgaatat agcgtggctc atcatgagat ccaggaaaac caattgtagg
      241 accaacctgc tgtaataatc ctttattttg agtaaaccaa ttgtcatcct gttgccataa
      301 gttatatgaa taaccatagc caccttcggg gcccggcatc cagaacacac tattcattgt
      361 gtgggttaca atacctttca aagcaagatt ttcttccata taacctctgc ctatcgtttc
      421 tgcaataata agtgggtcac gttgaaattt gtgttggcaa gcctcctttg cgatttctgt
      481 tagtcgagac aataccttgt tatttaccat ggctggggca tcaatacgta ttccctgaaa
      541 accaagatgc tcaatattac gctcgataaa tggtttccaa taatattcga tgatttgttc
      601 gcaaatgaca gggtcatcat agttaaacgt tgctacatca ctccagggat tattggccaa
      661 cggattgtag ttttcgtcca tgttatgcat caccaaattt ccattggggt gacgtttaaa
      721 ccattttttc gtatcaatat tgtatttttc aagaaaaaat ttatcgccat tcacaaaacg
      781 atgatctacc gccaggtgtc ttgcgaccaa atcaaatata ggcattaaac caagctcatt
      841 tgcttttgca gtgtattgct gaacgctttt atcattcttg gcatagcgtg gataaattga
      901 ttcatcctgc atggcataag ggctatgaat acgattagcc atgttaggga caggattata
      961 ttgacaaggt gtataaaatg ggttgaccca aacttgctta aaacccatct tctgaattcg
     1021 ctgcaaatct tcccgcatcg catcaaaatc ttgatataag ccagggtatt ggttatagca
     1081 acaaatccgt ggagacatgc tgacttctca atttctgttc ctatgtatat gaagtatagt
     1141 agaagtatta aattcgacct tgttgtatta ttttcaatta tttaattcct atgcatgctt
     1201 agaaaattaa ccaaagtgtg ctgtttgctc tttatgggca tcatatttac ttttattaaa
     1261 cattttgtta tattttgtac tatgtgattt aaaatttaat caatttagca gatatcctat
     1321 gtcataccaa aaaatagaac tcagtatttc attatcatta gattctccaa aattagatga
     1381 gtctgatttt atattgctct cagttaagta tcttgaaaag tcacttggca aaaagaaaga
     1441 gttttctggt ttttttgaag acatagaaca gctctatttt aagcagaatt acaaggaagc
     1501 aattgaaaaa atattggatt tctctaaaaa aaatgaatcc ttgttaagtg agcaagttgt
     1561 ccagaggctg gcagaggtcg ctcccagatt aaaaagcaat cctaaagaca atgaaagcag
     1621 aaggctttat gaaaccttgt atgcagatca ccttgaatca gtcattaaac aagagagtga
     1681 tctttccgta tttaacgaat tgagagacag ttataatgcg gtgaaacctg agtatgcagt
     1741 aactcatgag accgaaatca aaactttgga tgaagcaaag caatttattt tatcttttgt
     1801 catgctgaat gataatgttg aattaccttt aaaagcacaa tcagagagat acccaaaaaa
     1861 ggacagatcc agagaagaat tggggaatac tccatctgcg aatccaggga ttatgaaacc
     1921 taactcacct aattttaccg ataacctggt gcctgtcaga gatgtgccca aaatagccat
     1981 taatgagaaa gtggcgggtg ggtattcaaa aaccaagcct actactcctt ttgtggcgtc
     2041 gctgtctggc acaacctact cacttatggt ggtgttaacg gactatattg aaaagcataa
     2101 aacagataaa gacattgaaa aaaaagtaaa ccagattatt aatttatgga tttccagtta
     2161 tatcaaagaa ggttatcaca gctacagtga ggttgtggat gttttgacag agccgtttct
     2221 tcaatctatt tttgataaag caaatattaa attaaattat ggtgtacttg aagacacgca
     2281 tgcagaattt cgtaaagctc aggattatgt ttttggtttg acaattcgat cagcaatgca
     2341 tcatgagtta caagagcgat ttaagaataa agaaaaactg caggaagagg taaagaattt
     2401 tgaatctggg ttaaataaac tcaaccagaa tgtcgaaaat acaggtcgcc atcgcgaggc
     2461 tctgaataaa ttaaacgagg tatttcagga ttggtctgcg ggcaaaaaaa gctatgatgc
     2521 ttttaaaggt gagtctaatc aactgattta tgatattgag tctgaagaac aaaaaagtaa
     2581 tcgtggtctt ggctccatgc tgaagaacct cgggaactat cttttatact tgattaccct
     2641 tcgttttttg aaagaagact atccaaaacc gtctagtccg gtcacaatgt tagtcactga
     2701 attaagaggc acacttgagc aaatagatca ttttcataaa accagcctct ctttaaaaaa
     2761 agcaacgcag cctgacccta aaaatacttc gccatctggt gagacaacag atgttctgaa
     2821 atcagctgtc gatactgata agcatgataa aaaccaggaa agtgaagtaa taaagacatt
     2881 agactctgag caactggagg aagaaaggca agatgacaaa atagctcgtt tacaatagag
     2941 ctgttgcata acccactcct ttcattttat tgccacgttg caaatgattc tgaactgctc
     3001 ttatgtaaac ttcactcctc gaaacgtttg cactttgcct taaactgaaa taatcagttt
     3061 gggcaagaag gctaaggagc atggggtata tgcatttaat taaaaaattt ataaatggct
     3121 aaaaaagtat ttgcaataaa actaggcagt tatatattta tcaagccata tccctgtttt
     3181 gaaaacggga tatcaggttt tcactgattg gaatagcagt cattatgtgt tattattata
     3241 gtcatattga ctaattaaat tcctttggtt gaagcaatag gtttgagatt gtttcagtac
     3301 aatcaacaca tctgtagggg aagatttcca gtaagcaatg cttattgtaa ggacttcgtg
     3361 gtaagaaaca ttaatacaat ttttggatta agtgagaata taatggccta tcaattattg
     3421 ctcagtaaag aaaccctgaa taaaatttta caatacaaac agaacctcga aaaagggctt
     3481 gccacgcccg gtaaattttt tcttgaagaa ttaagtaaac aagaaaaaag catctctgaa
     3541 atggatatta ccacatttac tcaactgtta atccagtcta aaaaaccaca ggtttttgct
     3601 gaaagtcagg tttatcatga tggaactgac tggacactgg aagaagagag cattcttggg
     3661 gatgttagtg tcaatatgcc agtcactatg tacaatgatg gtggacatgg ctcctctttc
     3721 aagaatcacc cagagccaat ttccggttat ttggcttatg tgccaggcgc cctcttggct
     3781 tcgggcagtg gaccgacttc agatatgaaa gaagtgctag acaatggcaa actaaatcaa
     3841 gacaaattaa acgctttgta cgagagacgt ttgttacctc aattgatcca ttttaatgag
     3901 ttagcgcgac aaaatgaaaa gcaggcagca attactattc ccggtatagg aacagggtgt
     3961 ttttcaggcg cttattatga tgtgattaaa ccctatgtca gaaacgcatt aatccatatt
     4021 cttgagaaac ataaagattc gttaccttac attgatatca ttcattatga cccttatatg
     4081 ggagatgaac ctgctgaaaa gaagattggt catatgtctt ttcgtgtgag tccttctggt
     4141 gtggttcgtg gcacaacggg gcaacttgac taccccttag gtagcaatcc agatacgcat
     4201 atccttgttt caattgttgc ctgggatcat ttttcctggc ccggaaatga ttattggggc
     4261 ggcgctcgtc aaacagatga tggtgtcaag gccgctagca cggataccat ggggcaagtt
     4321 acaggagcaa ctggtgtcta tgataaaaaa tggggtcgat atatgcctcc agaatcattt
     4381 actaaagacc gtaaaggtat gagtgactgg ggggattatg tcagagaaaa tggcattgta
     4441 tttaatggcc ctgttttagc tctggataaa agcggtaaat tggatacctt ggaaaatgtt
     4501 gcaagtcgtt cctccaaagc aaaagttgaa actacaacta caataagtga ccttgtgcgt
     4561 agtatgttca gtcttttttc tcatagtact acgacagagc catctccaac tgttaaagag
     4621 gaagaatcga aaaaatcggg tcctcagtaa taattttgac aaattcttat agggtaacaa
     4681 tgggtctgac ttttgaggtt ggactctgtt actacttgca ggacttacca acttgacgtg
     4741 gtcaagttat gtgtagtttc atgaaagatt gaataatagt atggagcctc tgctattttg
     4801 aataaatcgt aacaatttca ttctggctta ttacgaggca tttttaaatt ggcaggatac
     4861 atctattatt tcgagatgca acttcgaatc ttcttcaatg ataaagtacg ttcatatatt
     4921 tattctaatt ttataaaata gaacaaaaat tgtttgtttt ttcttctgta tatttattga
     4981 ataactctat ggtttcatcc tggttgatat ataaattctg agatatgagt tgggtaaatt
     5041 catcactgct atatagctcc tgagcaatgt ccaggcaatc ataatcacgt aatagtttaa
     5101 gtactgtatt gatgcgtctc aatactgtag gttcctgaag caacatgact cgtctcaaaa
     5161 tatcgcggcc atttgtatct tgcataagta acaaatcaat gaattccatc cccatcactt
     5221 tttcaattaa ttcaactgtt gaatgatctt cactcaacaa tgcgggcaat aagatgttac
     5281 gtcctttatt gtccttgtat ttaagaaacg ttgttgcgtc agctcccaaa atgttaataa
     5341 tgtgagataa agactcttta tcgccccaat atgcaatatt gaataaagga atatcttcat
     5401 catttaatcc ttttttcaat aatacaggat aatcttgcct tttaagatat ttgagagtaa
     5461 tgaagaaata ttctctacca catctacttg cagaatgaat gcaactccaa tcagtgtaag
     5521 tatggttttg gtacactaaa agagtactca gttttgtttt tcctaaagtt tcgtagagca
     5581 atctcagtac taatgaaaat cgttctacag gaaaatgtaa tagagagcta aaaaaattgt
     5641 tatcataaag atcacaatag cggctaactg ataataagtc atcacctaga gctctaaata
     5701 ttttttgtaa tagtttttca tcagcttctg caacaatgaa atgtaatgcc aaaaactcac
     5761 tggtctccat gtctgctgtg caaaaaccac gtttaaaaat tcttgccaat aaggaacttg
     5821 gtaattcagc gcattctgat aaaaaaccaa gcatttcttc agtaaactgt tgttcgccat
     5881 caggaaaact atctatgata attcgatggc cagattcaaa taaggcgtag atcaaaggaa
     5941 tgtttttttc tctaatagcc tctagtaatc ctggctcatt cctacatgca tgatttgatt
     6001 cacatttctt tttcattgct tgagttaaag ccaattgttt ttgataatcc tctttaaaga
     6061 ttgagtggtt agctaaagtt ggtgaatagg aaaatcgttt gataaataaa ggcctccaaa
     6121 cggcattatc aagtgataac tcataccaaa gtttacaaac catgctgaca actatgatat
     6181 tttgaatgtc taaattttta tccgcaaaaa ttt
//
LOCUS       DACTJE010000176         6194 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.177, whole genome shotgun sequence.
ACCESSION   DACTJE010000176 DACTJE010000000
VERSION     DACTJE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.177"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..410)
                     /locus_tag="I9760_002621"
     CDS             complement(<1..410)
                     /locus_tag="I9760_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAT5044370.1"
                     /translation="MQKNKQNGGQLHAIAAIFALIVSTTLCFIPILFVGLLKLFPNKN
                     WQSLCTRCVDRIATFWCGINNFYVAKAQKIHWEISGLNNLTPKDWYLVVANHQSWLDI
                     VILQRLFNRKIPVLKFFIKDQLKWIPLLGFSWWA"
     gene            complement(477..1628)
                     /locus_tag="I9760_002622"
     CDS             complement(477..1628)
                     /locus_tag="I9760_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HAT5044371.1"
                     /translation="MNKTHFDTRAIHAGQEPCQSTGAVMTPIYATSTYKQIAPGEHLG
                     YEYSRTQNPTRKAYEDCIASLESGQKGFAFASGMAAINTVIDLLDSGDHVLAMDDLYG
                     GTFRLFDKVKTRTSNLSFSFIDMSVPENIEAAITPKTKLLWLETPSNPMLKLANLRKI
                     AAIAKKHNLITVADNTFATPWIQRPLELGFDIVLHSATKYLNGHSDVISGVVVVGDNP
                     MLSDKIAFLQNSCGAVAGPFDSFLVLRSLKTLSLRMQRHCENANHLANWLNGHPKIEK
                     VIYPGLKSHPQYSLAKEQMNDFGGMISLVLKGGLEDAKRFLARCELFTLAESLGGVES
                     LIEHPAIMTHASIPVEQRKALGIEDGFIRLSVGIEHIDDLRADLEHALG"
     gene            1777..4092
                     /locus_tag="I9760_002623"
     CDS             1777..4092
                     /locus_tag="I9760_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5044372.1"
                     /translation="MRRQEINYEKVAKAAEKIKKRGIEPSVNEIRDELGLVGNHPQLS
                     ILLEEWYHNQPEFKRKSHTPLTENINLNTDEIREKNVELEKSISLLRATLESTADGIM
                     MVNGHGAVVDWNQKFVEMWRIPSYMMESGKESISFEYILEQLIDPQSLIADVQYLYQN
                     PEWQGELPELHFKDGRIYERFTQLQRVGSQIVGRVYSFRDVTQKRMALDELRIRERAI
                     EASTHGVVIIDVTKNENKVIYVNRAFERITGYGEQYALGKGLLTLLGSNLEEVNHKRI
                     ELAIRESKEETIEMESIKRNGEFYWCEISVAPVKDSFGYVKHYICILNDVTQRRDMED
                     QLLQQATYDSLTNLPNRVLLMDRVEQAILQARKNKAILAFLFLDLDRFKLTNDTLGHS
                     MGDKLLQAIANRLLIVTEDFDTVARLGGDEFVILLTDIDNMLEAETIAQNILKIIEKP
                     IQIDQHSLKITGSLGISFYPRDGDDYESLMKSADLSMYHAKDTGRNNYRVYEPEMNRR
                     VINHMQLDNALRDALKNDELFLVYQPLIDLKQSRVVGFEALMRWHSKILGLVSPADFI
                     PMAEENGMILEMGEWAMKQACMQVKEWHKAGFKNLSIAVNLSGRQFRQKNLPEVVSRV
                     LKSAGLQSRFLELELTESLLIEDIDHVVDTMYALKDMGTKLVIDDFGTGYSSLSYLKQ
                     FPVDKLKIDRSFITEMVSNQNDAAIAKAIINLGHSLNLQVLAEGVENEFQRDFITSHG
                     CDYAQGYFFKAPDTPENILEFLKSLSESKIK"
     gene            4394..5743
                     /locus_tag="I9760_002624"
     CDS             4394..5743
                     /locus_tag="I9760_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HAT5044373.1"
                     /translation="MKHITIIGAGLAGTLCGLYLARRGYEVELFESRPDIRNSPTDYG
                     RSINLALSCRGITALKAMNLLSEVNKIMVPMRARAIHEANGEIHYQPFGRHIDEYINA
                     ISRSDLNALLLNKAELCPNIKLHFNMKLHSLDIHNKKIKFENKNGDFVEASYHRLIGA
                     DGAPSHVRDMLKNEGIVSASRDFLSHGYKELSISKKHTKGMAREHLHLWPRDSFMLLG
                     NPNPDDSITGSLFLANEGKDSFAELNNEEKLHLFFKTQFPDAYAAMPNLVQEFFGNPT
                     GHLSTIQCSPWYYKDECLLIGDAAHGIIPFFGQGMNSAFEDCRILDELLDEYQDDWSR
                     VNSVFYEHRKVNTDAIAKMSMDNYHEIHSDIRNPKFILQKQIERELMLRYPEHYVSMH
                     VLVMFTNTPYAKAMAIGELQSGLLEQICFPITDIKELNWQEVEKLLSLYDKKLAKII"
     gene            5961..>6194
                     /locus_tag="I9760_002625"
     CDS             5961..>6194
                     /locus_tag="I9760_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044374.1"
                     /translation="MKTVQAYIDSKQHEFMNHPFFNILEELNSIEEISYFVPELTFWA
                     MTFQDILRINEERVTDPYLKKVARHHRLEDAGHE"
BASE COUNT         2033 a          993 c         1277 g         1891 t
ORIGIN      
        1 atagcccacc aggaaaagcc aagtagagga atccacttta actgatcttt gataaaaaat
       61 tttaaaacgg gtatttttct gttaaataaa cgttgaagaa tgacaatatc cagccaactt
      121 tgatgattgg caacgactaa ataccaatct ttaggagtta aattatttaa cccggagatt
      181 tcccaatgta ttttttgtgc tttagccaca taaaagttat ttattccaca ccaaaaagta
      241 gcaatcctat caacacaacg agtgcaaagg gattgccagt ttttattagg aaataatttt
      301 aataatccaa caaataatat ggggataaaa caaagagtag tggacactat caatgcgaaa
      361 atagccgcaa tcgcatggag ctgcccgcca ttttgtttat ttttttgcat gaaatacttc
      421 tcctatacac atctgtcctg gttcttttta acagtcagaa ccaggattta catcttttaa
      481 cctaaagcat gctccagatc ggcacgcaga tcatcaatgt gttcaatacc aactgataat
      541 cttatgaaac catcttctat acccagggct tttcgttgtt cgactggaat agaggcatgg
      601 gtcataattg cagggtgttc aattaaactt tctacacccc ccaggctttc agccaaagta
      661 aataattcgc agcgagctaa aaatcgtttg gcatcttcaa gaccaccttt aagtaccaaa
      721 gagatcatac caccgaaatc gttcatttgt tccttggcaa gagaatattg cggatgactt
      781 tttaatccgg gataaatgac tttttcaatc ttggggtggc cgttaagcca gttagccagg
      841 tggttagcat tttcacagtg cctttgcata cgaagagata atgtttttaa gcttctcaga
      901 accaggaaac tgtcgaaagg gccagctaca gcgccgcatg aattttgcaa aaaagcaatt
      961 ttatcagaca acatgggatt gtcaccgacc acaacgacac cactgattac atcagaatgt
     1021 ccgttcagat atttggttgc tgaatgaagt acaatgtcaa atccgagctc aagagggcgt
     1081 tgaatccagg gggttgcaaa ggtgttatcg gcaactgtaa tcaaattatg tttttttgca
     1141 atagcagcga tttttcgtaa attagctagc tttagcatgg gattggaagg ggtttccaac
     1201 catagtaatt ttgtcttcgg agttattgcg gcttcaatat tctcgggtac tgacatatca
     1261 ataaaagaaa aggataaatt agaagttcgg gtttttactt tatcaaaaag ccggaaagtt
     1321 cctccataaa gatcatccat ggcaagaacg tgatctccgg aatctaataa atcaataact
     1381 gtattgattg cagccatgcc cgaagcaaaa gcaaatcctt tttggcctga ttcaaggctt
     1441 gctatgcaat cctcataggc ttttctggtg gggttctggg tgcgtgagta ttcatagcca
     1501 agatgttcgc caggggcaat ttgcttatag gtggaggtag cataaatagg ggtcatcacg
     1561 gcccctgtac tttgacatgg ttcttgtcct gcatgaattg cacgtgtatc gaaatgagtc
     1621 ttattcattt tttattccca taaaaatatt taaattcaaa gaaataccaa taggtgatca
     1681 aaatactgat taaaaattat actttcggcg ccttttttgc taaagttcat ggtgtaaatg
     1741 ccgatatttt tatagagtca ttctagagag ggatgcatgc gtaggcaaga aatcaattat
     1801 gaaaaagttg ccaaggccgc agaaaaaata aaaaaaagag gcatagagcc gtccgttaat
     1861 gaaattcgtg acgaacttgg tttagttggg aaccatccgc aattatcaat tttgctggag
     1921 gagtggtatc acaatcaacc tgaatttaaa cgaaaaagtc acacaccttt gactgagaat
     1981 attaatttaa ataccgatga aattcgcgaa aaaaatgtag agcttgaaaa atcaatttcg
     2041 ctgttacggg caacactcga atccaccgca gatggtatta tgatggttaa tggccatgga
     2101 gccgttgtcg attggaatca aaagtttgta gaaatgtggc gaattccttc ttatatgatg
     2161 gagtcgggaa aagaaagtat cagttttgaa tacatacttg agcaacttat tgatccgcaa
     2221 tctttgattg ctgatgtgca atatctttat caaaatccag agtggcaagg tgaattacct
     2281 gagcttcatt tcaaagacgg acggatttat gagcgtttta ctcagcttca gcgcgtgggc
     2341 tcacaaatag tggggcgagt atatagtttc cgtgatgtta ctcaaaagag gatggctctc
     2401 gatgagttga gaatacgtga acgtgctatt gaagccagca cacatggtgt ggtgattatt
     2461 gacgtaacaa aaaatgaaaa caaagtcatt tatgtcaatc gagctttcga aagaataacg
     2521 ggatatgggg agcaatatgc tttagggaag ggtttgctta ctttgctagg ttcaaatttg
     2581 gaggaggtaa accataaacg tattgaactg gcaattaggg aaagcaaaga agaaacaatt
     2641 gaaatggaaa gtataaaacg aaatggagag ttctattggt gtgaaatcag cgttgctcca
     2701 gttaaagatt cttttggcta tgtaaaacat tatatttgca ttttaaatga tgtaactcaa
     2761 cgacgtgata tggaagatca actgttgcag caagcaactt atgattccct gactaattta
     2821 ccaaaccgtg tcttactgat ggatagagtg gagcaggcca ttctacaagc aagaaagaac
     2881 aaagcaattt tggctttttt atttttggat ttagatcggt ttaaactgac taatgatact
     2941 ttgggacaca gtatgggtga taaattatta caagccattg ccaatcgatt actcattgtt
     3001 acagaggatt ttgatactgt tgccagattg gggggagatg agtttgtgat attacttaca
     3061 gacatagata atatgctgga agctgaaaca attgcacaaa atattttaaa aattatagaa
     3121 aaacctattc aaatagatca acatagtttg aagattacag gtagtttggg aatcagcttt
     3181 tatcccagag atggtgatga ttacgaatca ttgatgaaaa gtgctgattt gtctatgtat
     3241 catgcgaaag atactggaag aaacaactat cgtgtctacg aacctgaaat gaataggcga
     3301 gttattaatc atatgcaatt agacaatgcg ttaagagatg ctttaaaaaa tgatgaatta
     3361 tttctggttt atcaaccatt gattgactta aagcaatcca gagtagtcgg attcgaggct
     3421 ttaatgagat ggcatagcaa aatattggga cttgttagcc cggcggattt tatacccatg
     3481 gcagaagaaa atggcatgat tttggaaatg ggtgaatggg caatgaagca agcttgcatg
     3541 caagtaaaag aatggcataa agctggtttt aaaaacttaa gtattgcggt gaatctttct
     3601 ggacggcagt ttcgacaaaa aaacttgccg gaggttgtat cgcgagtatt aaaaagcgct
     3661 ggtttgcaat cgaggttttt ggaactcgaa ttaacggaaa gcctgttgat agaagatatt
     3721 gatcacgttg tagatactat gtatgcattg aaagacatgg gaaccaagtt agtgattgat
     3781 gattttggta caggctactc aagcttgtct tatttaaaac aatttcccgt agataaactg
     3841 aaaattgatc gttcgtttat cactgaaatg gtaagcaatc agaatgacgc ggctattgcc
     3901 aaagcgatta ttaacctggg ccacagttta aatcttcagg ttcttgcaga gggtgttgaa
     3961 aatgaatttc aaagagactt tataacatct catggatgtg actatgctca aggatatttt
     4021 tttaaggctc ctgatactcc agaaaatata ttagaatttt taaaaagttt atctgaatcg
     4081 aaaataaagt aatgactaat tcattaagtg aagaattcct gttttctctc aaaactcaaa
     4141 tcccatcata aatctctcca gatcggtatg ttttttgcaa gcaaaggtta aattaatcaa
     4201 gtagtttctg ttggctttgt ttattttatc ttttatgttg tttatatgaa gggcaagtat
     4261 atagaacaac tcaaaatgac aggttgagcc tgagttttac ttttattcaa gcaatatatt
     4321 gattgaattt ttgtattttt taaggagatt cggaatagat gtcgtgttga tatagaatgg
     4381 gttgggagga taaatgaagc acattaccat aattggagcc gggttagcgg gaactttgtg
     4441 tggtttatat cttgctagaa gaggctatga agtagagttg tttgaatcaa gaccagacat
     4501 tcgtaatagt ccgactgatt atggccgctc cattaatctt gctttgtcgt gcagaggaat
     4561 taccgcttta aaagcaatga acctgttaag tgaagtaaat aaaattatgg ttccaatgcg
     4621 ggcaagagct attcacgaag ctaatggcga gattcactat caaccatttg gacgtcacat
     4681 tgatgaatat atcaatgcta tttctcgttc tgatctcaat gccttgcttt taaataaagc
     4741 ggaattatgc cctaacatta aattacactt taatatgaaa ttgcactctc ttgatattca
     4801 taataaaaaa attaaatttg aaaataaaaa tggtgatttt gttgaggcct cataccatag
     4861 actaattgga gcagatggtg cgccgtctca tgtaagggat atgctgaaaa atgaggggat
     4921 agttagtgca agcagagatt ttttatctca tggatacaag gagctttcaa ttagcaaaaa
     4981 acatacaaaa gggatggcta gagagcattt gcatttatgg ccacgtgatt cgtttatgct
     5041 attaggtaac cctaatcctg acgattcaat tacaggttct ttgtttttag ctaatgaagg
     5101 gaaagacagc tttgctgaac taaataatga agaaaaatta catttatttt ttaaaacaca
     5161 gttcccggat gcttatgcag ctatgccaaa tttggttcaa gaattttttg gtaatcctac
     5221 tggacatttg agtactatcc aatgctcacc ctggtactat aaagacgaat gcttattaat
     5281 aggtgatgcg gcacatggga taattccatt ctttgggcaa ggaatgaata gcgcatttga
     5341 agattgccgt attcttgatg aattactgga tgaataccaa gacgactggt cgcgagtgaa
     5401 ctctgttttt tatgaacaca gaaaagtgaa taccgatgcg atagcaaaaa tgtctatgga
     5461 taattatcat gaaattcatt cggacataag aaatccaaaa tttattttac aaaagcagat
     5521 tgaacgggag ctcatgttgc gatacccgga acattatgtt tccatgcatg ttttggttat
     5581 gtttaccaat accccttatg ccaaggcgat ggcaataggc gagttacaat ctggcctttt
     5641 agagcaaatt tgtttcccaa taactgatat taaggagtta aattggcagg aagtagagaa
     5701 attacttagc ttatatgaca aaaaattggc gaaaattatt tgatttaatt tatgtcattc
     5761 attttaaatg aaatttatag tcaaagtttt gtcagaaaaa gatagattca gggacatttt
     5821 tttgtcgcta cctgaggctt attagcgcaa agaataaaaa aacaacctaa ttctgatata
     5881 ttattgagca ttttgattaa aaagatggca cgccttctgc agtataaact tgtctaaata
     5941 aagtttatat ggagtctgtt atgaaaactg ttcaagccta tattgattca aaacaacatg
     6001 agttcatgaa tcatcctttc ttcaacattt tagaagaatt aaatagtata gaagaaatca
     6061 gttattttgt cccggagtta acattttggg caatgacttt tcaagatata cttcgaatta
     6121 atgaagaaag agtaactgat ccttatttaa agaaagtagc tcgacatcac agattagaag
     6181 atgcaggaca cgaa
//
LOCUS       DACTJE010000177         6110 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.179, whole genome shotgun sequence.
ACCESSION   DACTJE010000177 DACTJE010000000
VERSION     DACTJE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.179"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..650)
                     /gene="hisF"
                     /locus_tag="I9760_002626"
     CDS             complement(<1..650)
                     /gene="hisF"
                     /locus_tag="I9760_002626"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAT5044375.1"
                     /translation="MLKKRIIPVQLLLNNRLVKTVQFNTYRDVGNPVASSSVYNSQYA
                     DELIFLNISRENTSITPLLEIIDKVSSVCFMPLSVGGGIRTFEDARQLISLGADKIVL
                     NSACFDTPELITQIADHYGSQAIIAGVDVKWDMKKQCYTVYSNCGQLERNIDLTEHIN
                     LCIDKGAGELLIQSIDRDGMMQGYDIALAKQIMDIAQIPVIICGGAGHYNHLKEAF"
     gene            complement(644..1261)
                     /gene="hisH"
                     /locus_tag="I9760_002627"
     CDS             complement(644..1261)
                     /gene="hisH"
                     /locus_tag="I9760_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAT5044376.1"
                     /translation="MIGILDLKSGNLRSVANSVYELGFDYIVLEDKNHMDDITHLIIP
                     GVGHYYTAINHLNETGLIEVLNQFYESKRPILGICLGMQLLSSIGHEGGVITGLNFIP
                     GIVQRFAISPPLRIPHIGWNSVYFQKHHPVFEGIKDKRDYYFVHSYHYICEEENHVLG
                     TTEYGASFPSIINKQHVIGFQFHPEKSQKNGLALLENFCNWDGLC"
     gene            complement(1290..2456)
                     /locus_tag="I9760_002628"
     CDS             complement(1290..2456)
                     /locus_tag="I9760_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HAT5044377.1"
                     /translation="MKRCTKCGLPETYESIEFDSENICNICHQHTFKKSVINWDERKK
                     DLDKLIEQYRGKYEYDCIIPFSGGKDSTFTLLYLMREYKIKPLVVQFNHGFMREKLKQ
                     NNERTFKQLGVDVLSFTPNWKIVKRLMLESLIRKGDFCWHCHTGIFSYPMQVAIKYNT
                     PLIFWGEPSSEYTAYYDYRDNEIEEVNEERFNRFINLGITAEDMAGMIAHDTDMDPRD
                     LTPYTYPKLRDLKRLGYNSVCLGSYIPWNTKKQAAEIMDQLGWEGDQVEGMPWDKYSY
                     EKIECYMQGMRDYIKFLKRGYSRVTQMTALDLRAGTITKDEADKLIQEYEGQKPPSLE
                     LFLEYLELTEEEFNQIVLKTVVPPFIPPLDDMKPAPKTWDFNKWYREIKITDKV"
     gene            complement(2489..3316)
                     /locus_tag="I9760_002629"
     CDS             complement(2489..3316)
                     /locus_tag="I9760_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAT5044378.1"
                     /translation="MKLGIGTAQFGMDYGISNVRGQCSEDEIKKIIESAVTHGIQVID
                     TAVAYGSSEEVLGRILPQNTFDVVTKISTNQTFQNSLKRLNRKKIYALLAHSADHLLD
                     SEGEKLWKQMQQAKETDKVQKIGASVYNSNQIDQLLKKYPIDIIQVPINLLDQRLILS
                     GHLDRLKDLQIEIHARSVFLQGLLLLNPDTLPTRFSSIKPLLKKLHQDCAQTNLTIIE
                     AALGFVQSLTQIDKVIVGFASHNEFLDLIRAAEIKLQSINFSQYAINDNTILNPACW"
     gene            complement(3313..4479)
                     /gene="pseC"
                     /locus_tag="I9760_002630"
     CDS             complement(3313..4479)
                     /gene="pseC"
                     /locus_tag="I9760_002630"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HAT5044379.1"
                     /translation="MNNYIPYGRQDIDQCDIEFVIETLNSDWLTQGPRISQFESAIAN
                     YCVVKHAIAVSNGTAALHLICMALGIGAGDLVWTTPNTFVASANCARFCGANVDFVDI
                     DPKTYNLSVDALERKLKFAAETGKLPKLVIPVHFAGQSCDMEAIYSLSKKYHFYIVED
                     ACHAIGGNYKNYKIGSCQFSDATVFSFHPVKLITTGEGGMVVTNNDELNLKLRTLLTH
                     GITREPTLMNEEPHGPWYYQQITLGYNYRITDIQSALGISQLRKLNGYVNRRHELAKN
                     YDSKLQELPLVIPYQANYNYSAYHLYVIRLKLNTINKTRLSVFNELREAGIGVNVHYI
                     PVHLQPYYRQLGFKKGDFPEAEKYYEEAITLPLFPTLTEKQQDYIINQLNKILL"
     gene            complement(4495..5511)
                     /gene="pseB"
                     /locus_tag="I9760_002631"
     CDS             complement(4495..5511)
                     /gene="pseB"
                     /locus_tag="I9760_002631"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HAT5044380.1"
                     /translation="MFNDKTILITGGTGSFGRMFTSVILSRYKPKKIIIYSRDEYKQF
                     TMNQEFDFPCMRFFLGDVRDLQRLELAMKGVDYVIHAAALKQVPAAEYNPMECVNTNI
                     YGAQNVIYAALKNNVSKVMALSTDKAANPVNLYGATKLASDKLFIAANNLIGTANIQF
                     SVTRYGNVVGSRGSVLPYFKKLLAQGVKQLPITDFRMTRFWITLQQGVSFVLKNFERM
                     QGGEIFVPKIPSVKITDLAESLAPGISHSQIGIRPGEKLHEIMCPSDDARLTLEFDDH
                     FVICPTINFNHEVDYTRNGVGDRGHPVPEGFEYNSGTNPHFLNVEEIRKLNHQFEAET
                     VDFV"
     gene            complement(5591..>6110)
                     /locus_tag="I9760_002632"
     CDS             complement(5591..>6110)
                     /locus_tag="I9760_002632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044381.1"
                     /translation="GCGTGKNLFNAYSKLSINQIIGAEICPNAVELGNKISNQCNIAG
                     KFKIFDYFQPNDIYEVTCEISDKYMIFTSHSIEQIEFEKTNFIEQILKLKHPPEVIIH
                     FEPIFDSTDSNFFHKLHHVYTQKNLYNSDLLNVLQKYETEGYIKIIDIQKDIFGLSAF
                     NPTSIIAWKIQN"
BASE COUNT         1897 a         1098 c         1020 g         2095 t
ORIGIN      
        1 aaaaaagctt cttttaaatg attataatga ccagctccac cacaaataat taccggaatt
       61 tgggcaatat ccataatttg tttcgctaat gcaatgtcat atccttgcat catgccgtct
      121 cggtcaattg attgtattaa taactctcca gctcccttat ctatacacaa attgatatgc
      181 tctgttagat caatgtttct ttccaattga ccgcagttgg agtagactgt ataacattgt
      241 ttcttcatgt cccatttgac atctacacct gcaattattg cttgagaacc ataatgatca
      301 gcaatttgag ttattagttc gggtgtatca aagcatgctg aattaagtac aattttatca
      361 gcccctaagc tgattaattg ccttgcgtct tcaaaggtac gaattccacc gccaacgctt
      421 aaaggcataa aacaaacaga agaaacttta tcaatgattt caagcaaagg cgttatcgat
      481 gtattttctc tactgatgtt aagaaatatt aattcatcag cgtattgtga gttataaacc
      541 gaagaacttg caacaggatt tcctacatca cggtaagtat tgaattgtac tgttttaacc
      601 aagcgattat ttaatagtaa ttgtacggga attatgcgct tttttaacat agtccatccc
      661 aattacaaaa attttctaat aaagccagtc catttttctg gcttttttct ggatgaaact
      721 gaaaaccaat gacgtgttgc ttgttaataa tagaaggaaa tgatgctcca tactcagttg
      781 tacccaaaac gtgattttct tcttcacaga tataatgata cgaatgtaca aaataataat
      841 cacgcttgtc cttgattcct tcaaaaacag gatgatgttt ctgaaaatag acactattcc
      901 atcctatatg cggtattcga agaggaggac taatagcaaa acgttgcact attcccggaa
      961 taaaatttaa cccagtaatt actcctccct catggccaat tgatgacaac aattgcattc
     1021 ctagacaaat acctaaaatc ggtcttttac tttcataaaa ttggtttaat acttcaataa
     1081 gaccagtctc atttaaatga ttaatggctg tataataatg gcctacgcca ggaataataa
     1141 gatgagtaat atcatccatg tgatttttgt cttccaagac aatataatca aaacccagtt
     1201 catacactga attagcgacg gagcgtaggt ttcccgattt taagtctaga attccaatca
     1261 ttaatgcact ttgtcttagt tttattacat tatactttat cagtgatctt aatttctcga
     1321 taccacttat taaagtccca ggttttggga gcaggtttca tatcatctaa tggtggtata
     1381 aaaggaggaa ccactgtttt caaaacaatt tgattaaact cttcttcagt cagttccagg
     1441 tattccaaaa ataactctaa agaagggggc ttttgtcctt catattcctg gattagttta
     1501 tcggcttcat cttttgtaat ggttccagcg cgtaaatcaa gagctgtcat ttgagttact
     1561 ctactataac cacgctttaa aaatttaata taatcacgca ttccttgcat atagcattca
     1621 attttttcat agctgtattt atcccaaggc attccttcaa cttgatcgcc ttcccagcct
     1681 aattgatcca ttatttctgc agcttgtttt ttagtgttcc atggaatata agaaccaaga
     1741 caaaccgaat tataacccag tcgttttaaa tcacgaagct tagggtaagt atagggcgtt
     1801 aaatcacggg gatccatatc ggtatcatgg gcaatcattc ctgccatatc ttctgcggta
     1861 atacctaaat taataaagcg attgaaacgt tcttcattta cttcttcgat ctcattatct
     1921 ctataatcat aataggctgt atattctgag gaaggttctc cccaaaaaat taaaggagtg
     1981 ttgtatttaa tagcgacttg catgggataa gaaaaaatcc ctgtatggca atgccagcaa
     2041 aaatctcctt tacgaattag tgattctaac atgaggcgtt ttacaatttt ccaattgggt
     2101 gtaaaactta aaacatcgac ccctaattgc ttgaatgttc tttcattatt ttgttttaat
     2161 ttttcacgca taaaaccatg attaaattgg acgaccaaag gcttaatttt gtattcacgc
     2221 atcaagtata gcagagtaaa tgttgagtct ttgcctccag aaaatggaat tatgcaatca
     2281 tattcgtatt ttcctcgata ttgttctatc aatttatcaa gatctttttt acgctcatcc
     2341 caatttatta ctgacttttt aaatgtgtgt tgatggcaga tattacaaat attttctgaa
     2401 tcaaattcta tggattcata tgtttcaggt aaaccacatt tggtacatcg tttcatatta
     2461 taacctcaaa gtgatgcctc attaattttt accaacaagc tggatttaat attgtattat
     2521 catttatggc atattgtgaa aaattaattg attgcagctt aatttctgct gctcgtatta
     2581 aatctaaaaa ctcattatga gaagcaaagc caacgataac tttatctatt tgtgttaaag
     2641 attgcacaaa accaagagct gcttctatga ttgttagatt tgtttgggca caatcctgat
     2701 gtaatttttt taataagggc ttaattgatg aaaaacgggt tggtaatgta tctggattta
     2761 ataataatag tccttgcaga aaaaccgacc tggcatgaat ttcaatttgt aaatccttta
     2821 aacgatcaag gtgaccagat aaaatcaaac gttgatcaag taaattgatt ggaacttgta
     2881 taatatcgat aggatatttt ttaagtagtt ggtcaatttg attggaatta taaactgagg
     2941 ccccgatttt ttgaactttg tctgtttcct ttgcttgttg catttgtttc cataattttt
     3001 ctccctcgct atctaataag tgatcagcac tatgtgcgag tagagcatag attttttttc
     3061 tatttaggcg tttcagtgag ttttgaaatg tttgatttgt agaaatcttg gtaaccacat
     3121 caaaagtatt ttgaggcagt atacggccca atacttcttc gctgctgcca taagcgactg
     3181 ctgtatctat tacctgaata ccatgtgtta cagcggattc aattattttt tttatctcat
     3241 cctctgaaca ttgccctctg acattggaaa ttccataatc cattccaaat tgtgctgtac
     3301 ctattcctaa tttcataaaa gaattttatt gagttgatta ataatatagt cttgttgttt
     3361 ttctgtgagg gtagggaaca atggtaaagt tatagcttct tcgtagtatt tttctgcttc
     3421 tggaaaatca cctttcttga aaccaagttg gcgataatat ggttgtaaat gtactggaat
     3481 gtaatgaaca ttcaccccaa ttccagcctc tcgtaactca ttgaacacag aaagccgtgt
     3541 tttgtttatg gtatttaatt tgaggcgaat tacgtacaaa tggtaggcag agtagttata
     3601 atttgcttgg taaggaataa caagtggtaa ctcttgcaac ttactgtcat agttttttgc
     3661 taattcatgt cggcgattca catatccatt gagttttcgt aactgactaa ttcctaaagc
     3721 tgactgaata tctgtaattc ggtaattata tcctaaagtt atttgctgat aataccacgg
     3781 accatgaggc tcttcgttca taagagttgg ttcgcgggtt attccatgcg ttagaagggt
     3841 acgtagttta agatttaatt cgtcattatt agtaacaacc ataccacctt caccggtggt
     3901 aattaattta acaggatgga agctaaatac agtggcatcc gaaaattgac agcttcctat
     3961 cttataattt ttataatttc cgccaatggc atgacaagca tcttcaacaa tgtaaaaatg
     4021 atacttttta gacagagagt aaattgcttc catatcacaa gactgtcctg cgaagtgaac
     4081 tggtataact aatttgggta gttttcctgt ttcagccgcg aatttgagtt tcctttccaa
     4141 agcatcaaca cttaaattgt aagttttagg atcaatatca acaaaatcaa catttgcccc
     4201 acaaaaacgt gcacaatttg ctgacgcaac aaaggtgtta ggtgtagtcc aaaccagatc
     4261 tcctgctccg atgccaagtg ccatacatat taaatgaaga gctgcagttc cattgctcac
     4321 agcaatagcg tgtttaacta cacaatagtt tgcaatagct gattcaaatt gtgaaattct
     4381 agggccctga gttaaccagt cagagtttaa tgtttcaatt acgaattcga tatcgcattg
     4441 gtcaatatct tgacggccat aagggatgta attattcata tcaacacatc ttgattaaac
     4501 gaaatctact gtttctgctt cgaactggtg atttaatttt cttatctcct ccacatttag
     4561 aaaatggggg ttcgtaccag aattgtattc aaatccttct ggtactgggt gtcctctgtc
     4621 tccaactccg tttcgggtat aatctacttc gtgattaaaa ttgattgttg ggcaaatcac
     4681 aaaatgatca tcaaattcta atgtcaaacg agcatcgtct gatgggcaca tgatttcatg
     4741 taatttttca ccgggcctta ttccaatctg tgagtgagaa atgccaggag ctaatgattc
     4801 tgctaaatca gtgattttaa cggatggaat tttcggaaca aaaatttcac cgccttgcat
     4861 gcgttcaaag ttttttaata cgaaagatac tccttgttgt agagtaatcc agaaacgtgt
     4921 cattctaaag tctgtaatag ggagttgttt tactccttga gcaagcaatt ttttaaaata
     4981 aggcaatact gaaccacggg agcctacgac attgccataa cgagtcactg aaaattgtat
     5041 attggcagtc ccgatgagat tatttgcggc tataaataac ttatcagatg ccagtttagt
     5101 tgctccatac agattaacgg ggttggctgc cttatcagtg gaaagagcca tgactttgct
     5161 tacattattt tttaatgcag cataaataac gttttgcgca ccatatatat tagtattaac
     5221 acattccatt ggattatatt cagcggcggg gacttgtttt agtgcagcag catgaatcac
     5281 ataatccaca cctttcatag ccagctctaa gcgttgcaaa tctctgacat ctcccaaaaa
     5341 aaaacgcatg caagggaaat caaattcctg attcatcgta aactgcttgt attcgtctct
     5401 agagtagatg ataatttttt ttggcttgta tctgcttagg ataacagaag taaacattct
     5461 tccaaatgat ccagtaccac ctgttatcaa tatagtttta tcattaaaca tgtaaattta
     5521 ttgtccttta aaacataaaa gtgcatagta acaccttgaa taacaaggtt ccatataaaa
     5581 taaattagat ttaattttgt attttccaag caatgatact tgttggattg aacgcgctaa
     5641 gtccaaaaat atccttttgt atatcaatga tcttaatata accttctgtt tcatacttct
     5701 gcagtacatt taataaatca gaattgtata aatttttttg tgtatacaca tgatgtaatt
     5761 tatgaaaaaa attagaatcg gtagaatcaa atattggttc aaaatgtatt atgacttcag
     5821 gagggtgctt aagtttcaat atttgctcaa taaaattggt tttttcaaac tctatctgtt
     5881 cgatagagtg agatgtaaat atcatgtatt tatctgatat ctcacaagtg acttcataaa
     5941 tatcatttgg ctggaaataa tcaaaaattt tgaatttgcc agcaatattg cattggttac
     6001 taattttgtt gcctaactca accgcattag ggcatatttc agcgccaata atttggttaa
     6061 ttgataactt tgaataagca ttaaataagt ttttccctgt accgcaacca
//
LOCUS       DACTJE010000178         6070 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.180, whole genome shotgun sequence.
ACCESSION   DACTJE010000178 DACTJE010000000
VERSION     DACTJE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.180"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..387
                     /locus_tag="I9760_002633"
     CDS             <1..387
                     /locus_tag="I9760_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAT5044382.1"
                     /translation="TFKRTPASIMNFVEGTRFTKVKKEQQQSPYNYLLKPKAGGISFI
                     INSMGQQIKSLIDVTIVYAEKNHSLWDFLCKRVKKVKIIIRQLPIPAQFTSANLINDF
                     QAQNAFKEWLNEQWAIKDNLIASLQE"
     gene            391..1221
                     /gene="dapD"
                     /locus_tag="I9760_002634"
     CDS             391..1221
                     /gene="dapD"
                     /locus_tag="I9760_002634"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAT5044383.1"
                     /translation="MNSLQALIEQAFENRQNLSLNTVSSDLINAINEVLSGLDNGQFR
                     VAEKINGEWTVHQWLKKAVLLSFKLFPNQIIDAGFCKFYDKIPLKYTDSSNEQFQQSG
                     VRVVPHAMVRRGAYIAKNTVLMPSYVNIGAYIDEGVMVDTWATVGSCAQIGKNVHISG
                     GAGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEKNSVISMGVFLGQSTKIYNRI
                     TGEVSYGRIPAGSVVVAGNLPSHDGSHSLYCAVIVKQVDEKTRAKVSINDLLRANQDD
                     "
     gene            1214..2347
                     /gene="dapE"
                     /locus_tag="I9760_002635"
     CDS             1214..2347
                     /gene="dapE"
                     /locus_tag="I9760_002635"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAT5044384.1"
                     /translation="MTDITQILTDLIGFPSITPEDAGCQKYMIQFLEQLGFTCQQLNN
                     GPVSNFFACYGKIGPLLVFAGHTDVVPVGEVSKWDTDPFSLEEKNGMLYGRGVADMKG
                     SLACMLHMARRFIKTYPSFPGRLGFLITSGEEGDEFNLGTPYVMQKLEQQGIVIDYCI
                     VGEPSSSLKTGDVIKIGRRGSLSAKIHLSGKQGHVAYPHLADNPIHRISPVLAELTSM
                     QWDNGNAYFPPTSMQITYIHCGGHAGNIIPGELNLHLNFRYSTEQTDESLKTRVINAF
                     THHKLNPTIEWRFNGEPFLTNKGILLESCKQTVLEHIGTLPELSTSGGTSDGRFIAPY
                     GVEVIELGLVNATIHQVNECTSLQDLNTLETMYFSICEKLFID"
     gene            complement(2647..3927)
                     /locus_tag="I9760_002636"
     CDS             complement(2647..3927)
                     /locus_tag="I9760_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAT5044385.1"
                     /translation="MCAAHHQKNKYIFSTPLIYAAMIGLGIISGLSDLPFLKETGLLI
                     SDLFIKLFKCISLPIISLSIIVTLANYQTDSLMKSVWQRTIKYTFATTLVAALISCFL
                     YILIHPSTVQVNLDAANPKSTSSLGYLGYLSNMIPTNLLSPFLEQQVMGVLLLSIVIG
                     FAVRQIPETEPRETIIRFFRGAHGMFLVMTKWIIKLIPLGLFGFITSTVIQLRSGMDI
                     KGIGQYLMIIVLANLIQGLVILPLWLKSNGIKPFSSMRAMLPALSVAFFSKSSVGTLP
                     VTMNTVEANLKVNPQISRFVLPLCTSINMNGCAAFIFATVIYLMQSHGIQVSYATMGI
                     WVLVSTIAAIGNAGVPMGCFFLSVSLLSSMNVPITLMGVILPFYGLIDMLETALNVWS
                     DACVTKVVNDKAEGGDGVVGIRPKERSLLNAELG"
     gene            complement(4443..5519)
                     /locus_tag="I9760_002637"
     CDS             complement(4443..5519)
                     /locus_tag="I9760_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HAT5044386.1"
                     /translation="MITIRNKIGQMLIMGFHGSELHDNSPVAQWLSNDGLGGVLLFDK
                     DLSTGIYGKNLRNQAQIRQLIRQLNRYASMISGGNDNLPLFTAIDYEGGVVDRLSKIE
                     GCPPTMKARELAKLSSDDFDEEVTQMAMTLQSLGFNLNFAPVVDLNLNAHAGIIGNLG
                     RSFSSNPEVTIRLAKQFVDVFSRHGIACAYKHFPGHGSATGDTHEGFVDVTETFQTDE
                     LIPYRSLLKDLSNPVMVMTAHVINKHLDSKGLPATLSYDVLTLLLRQSIGYDGVIISD
                     DLQMHAISNHYSLEEALCLTINAGADMVIFANQLGTITAPEVIDVIEKLVIDKQIPSQ
                     RIDEAYRRIVRLKQQIICSALINT"
     gene            complement(5606..6052)
                     /locus_tag="I9760_002638"
     CDS             complement(5606..6052)
                     /locus_tag="I9760_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044387.1"
                     /translation="MYLRLLALSALCFVTSPIWSFTCIYTLVKDNCWTDYDVTVDVIE
                     DSTSKTLLTITAPKGKSWARGTFNCEAAEGLRYVAQFSPVFWQNDVGKTYPALRNWYL
                     PEKVNPGDLAWTIPVCFPADFAQVPFPPNVAGNCKCNFKNIPDPKL"
BASE COUNT         2002 a         1196 c         1125 g         1747 t
ORIGIN      
        1 acttttaaaa gaaccccggc ttctattatg aattttgtag aaggaacccg ttttactaaa
       61 gtaaaaaaag aacagcaaca atccccttat aattatttat tgaaacccaa agcaggcggt
      121 attagtttta ttattaactc gatgggacaa cagataaaaa gcctgattga cgtcactatc
      181 gtttatgcag aaaaaaacca ttctctctgg gattttcttt gtaaacgagt caagaaagtt
      241 aaaatcatta ttcgtcaact ccctatccct gcacaattta catcggcaaa tcttatcaac
      301 gattttcagg cacaaaatgc attcaaagaa tggttaaatg aacaatgggc aataaaagat
      361 aatttaatag cctctttaca ggaataaaac atgaactcac ttcaagctct aattgaacaa
      421 gcctttgaaa accgtcaaaa tttatcgctc aacacagttt cttcagattt gatcaatgcc
      481 attaatgaag ttctcagtgg tcttgataat ggccaattca gagtcgctga aaaaataaat
      541 ggtgaatgga cagttcatca atggcttaaa aaggcagttc tcttatcctt taaacttttc
      601 ccaaatcaaa tcatagacgc gggtttttgc aagttttacg ataaaatacc gctaaaatat
      661 acggattcta gtaatgagca atttcaacaa tcaggggtaa gagttgttcc tcatgccatg
      721 gtgcgacgtg gagcatatat tgccaaaaat actgttttaa tgccttctta tgtcaatata
      781 ggtgcttata ttgatgaggg agtaatggtt gatacctggg caactgttgg ttcttgcgcc
      841 caaataggaa aaaatgtaca tatatctggc ggcgcgggta ttggaggtgt tttagaacca
      901 ttacaagcga acccgaccat cattgaagac aattgcttta ttggtgctcg ctctgaaata
      961 gtggaagggg ttatcgtaga aaaaaactcc gtcatatcaa tgggagtatt tttaggccaa
     1021 agcactaaaa tctacaatcg aatcactggc gaagtaagct acggcagaat tccggctggc
     1081 tcagtagttg ttgcaggtaa tttgccaagt cacgatggaa gccacagttt atattgtgct
     1141 gtcattgtta aacaagtgga tgaaaaaact cgcgcgaagg taagcattaa tgatttacta
     1201 agagccaatc aagatgactg atattacgca aattctgact gacctcattg gctttccttc
     1261 tattacacca gaagatgctg gctgccaaaa atacatgatt caatttctgg agcaattggg
     1321 atttacttgt caacaactga ataatgggcc cgtatccaat ttttttgcct gctatggcaa
     1381 aattgggcct ttattggtgt tcgcaggcca taccgatgta gttcctgttg gtgaagtatc
     1441 aaaatgggat acagaccctt tctctctcga agaaaaaaat ggcatgctct atggtcgtgg
     1501 ggttgctgat atgaaaggca gcctggcctg tatgttgcat atggccagaa gatttattaa
     1561 aacttatcct tcctttccag gcagactagg atttctgatc accagcggcg aagaaggcga
     1621 tgaatttaat ttgggcaccc cttatgtgat gcaaaaactg gagcagcaag ggattgttat
     1681 tgattattgc atagtaggtg aaccttcaag cagcttgaaa actggtgatg tcatcaaaat
     1741 tggaagacgt ggatcattaa gtgcaaaaat acatctgagt ggaaagcaag gtcatgttgc
     1801 ctatccgcat ttggctgaca atccaattca tagaattagc ccagtattag ccgaactcac
     1861 ttcaatgcaa tgggataatg gcaatgctta ttttcctcct acctccatgc aaataactta
     1921 tattcattgt ggagggcatg cgggtaatat tatcccaggc gaattaaatc tgcatttgaa
     1981 ctttagatat tcgacagagc aaacagacga gtcattaaaa acgagagtaa ttaatgcctt
     2041 tacacaccat aaacttaacc ccactataga atggcgattc aatggcgaac cattcttaac
     2101 gaataaaggc atattgctag aaagctgcaa acaaacagtg cttgaacata ttgggactct
     2161 ccccgaactt tcaacaagtg gtggaacttc agatgggcgt tttatagcac cttatggggt
     2221 tgaggtcata gagctgggac tggtaaatgc aaccattcat caggttaatg aatgcacttc
     2281 tctacaggat ttgaatacac ttgaaaccat gtatttttca atctgtgaga aattattcat
     2341 tgattaatca aaccaattct gtaatgaggg ttcgcaccaa cagtccaaat tacagtaata
     2401 ggttagtaac ctcatcttta aatatcctct ggcggtcgga tttgagttca gtttggatga
     2461 aaataatcct gaaaaagcac agcatagata gttatgtgag aattttgaag gattattttc
     2521 gttcaggatg aaccaaatac ggccgttcca ggtagtgagc tgatttcata gacattaagt
     2581 ctccaaaaaa ctttctggag acttatttgt aagctatagt catttgcgct tgaagatttg
     2641 ctggttttaa cctaactcgg catttaataa tgaacgctct tttggtctaa tgcccactac
     2701 tccatcacca ccttctgctt tatcattaac aaccttggta acacaagcat cagaccatac
     2761 atttaaggca gtttccaaca tatcaatcaa accatagaaa ggaagaataa ctcccataag
     2821 tgtgataggc acattcatac tggagagcaa actaacgctt aagaaaaagc agcccatagg
     2881 aacgccggca ttccctattg ccgctatagt tgatactaaa acccatatcc ccatagtagc
     2941 ataagagact tggatgccat gactttgcat aagataaata actgtggcaa aaataaatgc
     3001 ggcacatcca ttcatattaa tgcttgtgca caatggtaaa acaaaacgac tgatttgagg
     3061 gttaaccttt aaattagcct ctacagtatt cattgtaaca ggcaaagttc cgacagatga
     3121 cttggaaaaa aaagcaacag ataacgcagg taacattgcc cgcatcgaag agaatggttt
     3181 aataccattt gatttcagcc ataaaggcag aatgactaat ccttgtatca aattagctaa
     3241 aacaataatc atcaagtatt gacctatccc tttaatatcc atacccgatc gcaactgaat
     3301 tacagtagaa gtaatgaacc cgaataaccc caaaggtatt aatttaatta tccatttggt
     3361 catcactaaa aacatcccat gagcaccgcg gaaaaatcga ataatagttt cacgtggctc
     3421 tgtctccggt atttgacgaa cagcaaaacc gataacaata ctaagcaaca atacccccat
     3481 aacctgttgc tccaggaatg gagataataa attagtcggt atcatgttac tcaaatagcc
     3541 aagatatccc aaactgctgg tggattttgg gttcgccgca tccaagttga cttgaacagt
     3601 gcttggatga atcaaaatat atagaaaaca gctgattaat gctgcaacta aagttgtagc
     3661 aaaagtatat ttaattgttc tttgccatac acttttcatc aaactatccg tttgatagtt
     3721 tgccagagtt acaataatcg acaaggaaat aataggtaaa ctaatgcact tgaacaattt
     3781 aataaacaaa tcagaaatta aaagccctgt ttcttttaaa aaaggcaaat cggataatcc
     3841 acttattatc ccaagtccaa tcatcgctgc ataaattagt ggggtactaa aaatatattt
     3901 attcttttgg tggtgtgctg cacacatagc tgaatcctta aaactttagt taattattta
     3961 agcaaagtat gattagcagc aacaacagat ggcttgatat atggagtgaa ataatgagga
     4021 attataataa tataatgatt gaattgacat aaaagtcatc acccatagct aaaactgttc
     4081 atttagagta catattgtaa cacgattttt cttctagtca ataggataaa taaactgagt
     4141 caataataca gggcttacga aaaatcccaa ggtcaaggca aaaaatgttt ttaatgaggg
     4201 agtttagata aactaaatga ctgaattaaa aactttttta acaaagagat tggggttttt
     4261 cgtaagtcct gttatattct tcacagaaca caccatatag atactaatta aatacttcaa
     4321 ccccaatctc aaacaattgt gtctgttcat gctgaaaaag aagatggcgc ctctcaaata
     4381 ggcggaatta tagtggtaac ttgatgtttc ataaatccgc cagttaccac ttggcatggg
     4441 cattacgtat tgattaatgc actgcatata atttgttgct tcagacgtac tattcttctg
     4501 tacgcctcat caatacgttg agagggaatt tgcttgtcaa ttaccagttt ttcaatcaca
     4561 tctataactt ccggtgcggt aatagttcca agctgattgg caaatatgac catatcagcc
     4621 ccggcattga tggtaagaca aagagcctct tctaatgaat agtgatttga aatggcatgc
     4681 atttgcaaat catcgctgat aatgaccccg tcatatccta tgctttgacg taacaataac
     4741 gttaaaacgt cgtaggacag agttgctggc aatcccttac tatccaaatg cttattgatc
     4801 acatgtgccg tcattaccat gacagggtta gacaggtctt ttaataaact tcgataagga
     4861 attagctcat cggtttgaaa ggtttctgta acatcgacaa atccctcatg tgtatctcca
     4921 gtggcgctgc catggccggg gaaatgttta tacgcacaag caataccatg acgactgaaa
     4981 acatcaacga actgcttggc caaacgaata gtcacctctg ggttagaaga aaagcttctt
     5041 cccaggtttc caataatccc tgcatgagca tttaaattta aatcaacaac cggggcaaaa
     5101 tttaaattaa aacctaatga ctgcaacgtc atagccattt gagttacttc ttcatcaaaa
     5161 tcatcactgg ataactttgc aagttccctg gctttcatag taggcggaca gccttctatt
     5221 ttgcttagcc tgtcaaccac tccaccttcg taatcaatcg cagtaaataa aggcagattg
     5281 tcattacctc ctgaaatcat tgatgcataa cgattaagct gtcgaatcaa ttgccttatc
     5341 tgagcctgat tccttaaatt cttgccataa atccctgtgg atagatcttt gtcgaataac
     5401 aacactccgc cgagaccatc attagataac cattgagcaa ccggactatt atcatgcagc
     5461 tcggaaccat gaaatcccat aatcagcatt tgtccgattt tgtttcttat tgtaatcaat
     5521 cactcgctcc atcacaaaat aagcataaaa tcatacacaa agatattctc tctggctatg
     5581 ccccgctacg cattagcttt taaaattaaa gcttgggatc aggaatgttc ttgaagttgc
     5641 atttacagtt tcctgctaca ttgggtggaa agggaacttg agcaaaatct gccggaaaac
     5701 aaacggggat agtccaggcc aaatctccag gattcacttt ttctggtaaa taccaattcc
     5761 ttaatgccgg gtatgttttt ccgacatcat tttgccaaaa gacaggcgaa aattgagcga
     5821 catatctcaa cccttcagca gcctcacaat tgaaagtacc tctagcccat gattttcctt
     5881 tgggagcagt aattgtcaac aaagtttttg acgtagaatc ttcaatgaca tcgacagtaa
     5941 catcataatc agtccaacaa ttgtctttaa ccaaagtata gatacatgtg aaagaccaaa
     6001 ttgggctagt cacaaagcac aatgcagata gggcgagtaa acgtaagtac atgattcaat
     6061 cctaagattt
//
LOCUS       DACTJE010000179         6043 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.181, whole genome shotgun sequence.
ACCESSION   DACTJE010000179 DACTJE010000000
VERSION     DACTJE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.181"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..881)
                     /locus_tag="I9760_002639"
     CDS             complement(<1..881)
                     /locus_tag="I9760_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAT5044388.1"
                     /translation="MNFFRCEKPIYIKGPFVALPERIMSNQDVLNWMNSTQNPTVIGF
                     STGIKNRHWVNEDQACSDLAVRAAEHLFMEKTREKHKVNQVILATISGDYPSPPSSPL
                     VQYRLGLQNAGAFDIGAACAGFVVGLHTSAALAQTNDGSVLLIASEIRSKFLNKNNFA
                     TSVLFGDGAAACCVSQDKEEADFRFIASALFADGEVYDAVSTPAGGSRLPAAVCNDHE
                     QFYITIKESTALFVKAVHGMADSAKDFLKELNLTISDIQWLVPHQGNKNLVLSVAKQL
                     GFPEEKTIKTVEETGNTS"
     gene            complement(878..1630)
                     /locus_tag="I9760_002640"
     CDS             complement(878..1630)
                     /locus_tag="I9760_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAT5044389.1"
                     /translation="MNILITGGSSDIAQAIAKRRSESGDKIIITCSNDISLNQTLAIY
                     RQQNIDVTGFIYNFSDPQACEADLELILKEPLDGIILNAFTRVTQLRKLHALPYHAVR
                     TYLDNNLNGNIWLIHRLLPALLKNKFGRLVLISSLSATAGTSRYPVYCTAKAALEGLF
                     FNLAVDYSANNILSNIVRVGLIKTSRTEQFWKNPAYQEKVIQIIPQGTMGEPTQVAEA
                     IDPLLSKTSFMTGSVVTVSGGLPLMRSEGLLS"
     gene            complement(1627..1860)
                     /locus_tag="I9760_002641"
     CDS             complement(1627..1860)
                     /locus_tag="I9760_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAT5044390.1"
                     /translation="MLSNRIEQVLERIGLTNLPKNKQAKLEQGGLDSLMLALLIIELE
                     REFEIKIPVIPLEKEHYESINTIEQYLIELGAK"
     gene            complement(1850..3229)
                     /locus_tag="I9760_002642"
     CDS             complement(1850..3229)
                     /locus_tag="I9760_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HAT5044391.1"
                     /translation="MLFLAPDVPNKVAITQGNRVITFDELRHEVLANSKQLMKLPKTI
                     IVLHATPEIGFIIQLLACLETNRPIALFPNSISEEEKQRRLSLLGNAVMINEEGELQE
                     ICENKTIQPHPQTALILFTSGSTGAVKAVQLSSINIKANCHAVIKALEFKKVQDQLLF
                     LPLSYSFGLLGQLLPGLMFGLTTRLITHFTDIKMLMEQGTVPQMWSGVPSHWVAINAI
                     AKRYPDSAAKINAIVSAGAPLSTTLRADLKQIFPNAIIYNNYGLTEASPRVLTYSSKD
                     PLFTENYAGYPVGDWKVKLSAEHELLISGTQMMLGYLGEKDSTKIQNGWLSTGDIAEI
                     LPNGLVAIKGRRDSLVNIGGEKVNLSEIEQAICQIDVIKEAIVIPQEDKIYGTRLIVC
                     IEKKSLPSSISEQNLIEKIQTHLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIDGL
                     DNKEKNNAK"
     gene            complement(3234..4979)
                     /locus_tag="I9760_002643"
     CDS             complement(3234..4979)
                     /locus_tag="I9760_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HAT5044392.1"
                     /translation="MKKEYLQCQSLVDVVRLRALHSPNKKSCTFLNKELEETMTYEQL
                     DQHAKAIAATLQAEGAKPGDRVLLLFAPGLPLIQAFLGCLYAGCIAVPIYPPAQEKLL
                     DKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPKFLKIPAIALESIELNRSSSWQPT
                     SIKSNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHD
                     MGLIGCILTPIYGGIQAIMMSPFSFLQNPLSWLKHITKYKATISGSPNFAYDYCVKRI
                     REEKKEGLDLSSWVTAFNGAEPVREETMEHFYQAFKEFGFRKEAFYPCYGLAEATLLV
                     TGGTPGSSYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQEVKIIDPDTLIPC
                     DFDQVGEIWVQSNSVAKGYWNQPEETRHAFAGKIKDDERSAIYLRTGDLGFLHENELY
                     VTGRIKDLIIIYGKNHYPQDIEFSLMHSPLHHVLGKCAAFVIQEEHEYKLTVMCEVKN
                     RFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCRKHLLDK
                     TLPIVATWQLNKIEE"
     gene            complement(4991..>6043)
                     /locus_tag="I9760_002644"
     CDS             complement(4991..>6043)
                     /locus_tag="I9760_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HAT5044393.1"
                     /translation="LKPNIQFEITGASRILPASGPITNLEILKNFPRTADKSQGFLEK
                     FAEKIGEEFGFHTRYWCHKPWESLDNSRELSSESLAMQAVDKLITHSMSKDVDAFLLG
                     STTNKRFTGSQAAAVLGNLKLNAPAYDLKTGCSTSLSTLHFAYALMALGYQKILVCCS
                     ETLSKVIDPENEKTWIGLADGAASLLLEKSKEGSFTIEKSFFSTDGQYVNAFTTQGVF
                     PPTHEQIDSVGYHLVGDETLMKELAYSRYMQMLDHLLPTEKEKNEITWVIPHQVNRKL
                     IDQVLHENNLNNKIIIWDADTIGNIGGASVLYTLARAVEEKLFDRSGKILLMSVGGGL
                     SYAGQVLNYQKASYSN"
BASE COUNT         1672 a         1222 c         1226 g         1923 t
ORIGIN      
        1 gaagtatttc ctgtttcttc aacagttttt atcgtttttt cttcaggaaa acctaattgt
       61 tttgctaccg acaaaaccag atttttattt ccctgatgag gaacaagcca ttggatatca
      121 gagattgtca gatttaattc ttttagaaaa tctttggcgc tatctgccat tccatgcact
      181 gcttttacaa ataaagcagt actttctttt atggtaatgt aaaattgctc atgatcattg
      241 caaaccgcgg cgggcaagcg ggaacctcct gccggagttg agacggcatc gtatacttca
      301 ccgtcagcaa acaacgcaga cgctataaag cgaaaatcag cttcttcttt atcttgagaa
      361 acacaacatg ctgctgctcc atcaccgaat aagacactgg ttgcaaaatt gtttttattt
      421 aagaatttgg agcgaatttc gctagctatg agcaataccg aaccatcatt ggtctgtgct
      481 aaagcagcac tggtatgcaa accgacaaca aatccggcac aggcagcccc tatatcaaaa
      541 gcccctgcgt tttgtaggcc aagtctgtat tgtacgagag gactgcttgg cggggagggg
      601 taatcccctg agatagtggc caggatcact tggttcactt tatgtttttc cctcgttttt
      661 tccataaaca aatgttctgc cgctcgcaca gccagatcgc tacaggcttg atcctcattt
      721 acccaatgcc ggtttttaat tccagttgaa aatccaatca cggttggatt ttgggtactg
      781 ttcatccaat tgagaacatc ttggttgctc atgattctct cgggaagcgc aacaaaaggg
      841 ccttttatat agatgggttt ttcacaacgg aaaaaattca tgacaacaat ccttctgaac
      901 gcattaaagg cagaccacca ctgactgtta caactgatcc cgtcataaac gaggtttttg
      961 acaaaagtgg atcaatggct tccgcaactt gagttggttc acccatagtg ccctgtggaa
     1021 taatttgtat tactttttct tgataggctg gatttttcca aaattgctct gttctggagg
     1081 ttttaattaa gcctacgcgt actatattag acaagatatt gttagcacta taatcaacag
     1141 ccaaattaaa aaataatcct tctagagcag cctttgccgt acaataaacc ggatatcgac
     1201 tggtgccagc tgttgctgaa agacttgaaa tgaggactag tcgcccaaat ttatttttta
     1261 gcaaggcagg taataaacga tgaataagcc aaatgttgcc attgagattg ttatccaggt
     1321 aagtacgaac agcgtgatag ggcaatgcat gtaatttacg cagttgggtg acccgagtga
     1381 acgcatttaa tatgatgcca tcgagtggtt cttttaaaat caattccaaa tcggcctcac
     1441 aagcttgagg atctgaaaag ttataaatga aacccgtcac gtcaatgttc tgttggcggt
     1501 atattgcgag agtttgatta agactgatgt cattagagca ggtaatgatg atcttatcgc
     1561 ctgattcact gcgtcgtttc gctatggctt gggcaatatc agaggagccg ccagtaataa
     1621 ggatattcat tttgccccca gctcaatgag gtattgttca atagtattga tcgattcgta
     1681 atgttctttc tcgagtggaa tgacagggat tttaatttca aattcacgtt ccaactcaat
     1741 gattaacaag gccagcatca aactatccaa gcccccttgc tcaagttttg cctgtttgtt
     1801 tttaggaaga ttggtaagac caattcgttc cagtacttgt tcaattcgat tacttagcat
     1861 tatttttttc cttattatct aaaccatcaa tattcaaaac caacgctttt ctatcaagct
     1921 tcccatgttg atttctgggt agggatgaca tgagctgtaa ttgaataggc aatttcctgg
     1981 gtagtagatg cgtttgtatt ttttctatta aattttgttc agaaatggaa gaaggcagcg
     2041 attttttttc gatacagaca attaatcgtg tcccatatat cttgtcttcc tgagggataa
     2101 caatggcctc ttttattaca tcaatttggc aaattgcttg ctcgatctca gagaggttaa
     2161 ctttttctcc cccaatatta acaagactgt ctcgacgtcc tttgattgca actaacccat
     2221 tgggtaatat ttccgcaata tctcctgttg ataaccagcc attttgtatt ttagtactgt
     2281 ctttttcacc aagataaccc agcatcattt gagtgccgct aattaataat tcatgttcag
     2341 cagaaagttt gaccttccaa tcgccaacag gataacctgc ataattttct gtgaataaag
     2401 gatccttact ggaataagtc agcactctcg gagatgcttc agttaaaccg taattgttat
     2461 agatgatagc gttaggaaat atttgtttca aatccgctct gagtgtagtg gataaaggag
     2521 cgccagcaga aacaatcgcg ttaattttgg ctgcgctatc agggtatcgt tttgctatgg
     2581 cattgatagc tacccagtga gaaggtacgc cactccacat ttgaggaacg gtaccttgct
     2641 ccattagcat tttaatgtct gtaaaatgag tgattagtcg agtggtcagg ccaaacatta
     2701 aaccagggag caattggccc agaaggccaa aagaatagga taaaggtaaa aagagcaatt
     2761 gatcttgaac ttttttaaat tccagcgcct ttatgaccgc atggcagttg gcttttatgt
     2821 tgatgcttga tagttgaact gctttgactg ctcctgtgct tcctgaggtg aagagaataa
     2881 gtgctgtctg gggatggggc tggatcgtct tattttcaca tatttcctgc aattcccctt
     2941 cttcatttat cattacggca ttacctaaaa gagaaagccg tctttgcttt tcttcctctg
     3001 aaatggaatt ggggaaaagg gcaatggggc gatttgtttc caaacaggcc aatagttgaa
     3061 taataaatcc aatctcaggt gttgcatgga ggacaatgat ggtttttgga agtttcatga
     3121 gttgctttga atttgctaaa acttcatgcc ttaattcatc aaaggttatt actcgattgc
     3181 cctgagtgat tgctacttta tttggaacat ctggtgccaa aaacagcatg gttttattcc
     3241 tcaattttat tgagttgcca ggtagccact attggcagag ttttatccaa aagatgtttt
     3301 cgacaaaaat tcctgcgaat ttttccgctg gtagtatgtg gcattgcttt aagaggaatc
     3361 aggactatag tatgtacctc caattggtgg ttttcgtaaa caagctcaaa aatctcattg
     3421 aataaattgt cttgagctac gtcatccatg aatcgatttt ttacttcaca catcacagtc
     3481 agtttatatt catgctcctc ctgaatcaca aaagcagcgc atttgcccaa tacgtgatgg
     3541 agcggagaat gcatcaggct gaactctatg tcctgaggat aatgattttt accataaata
     3601 ataattaagt ctttaatgcg tccagtaacg tataactcat tttcatggag aaagcccaag
     3661 tccccggttc ttaaatagat tgcgctacgc tcatcgtctt taatttttcc tgcgaacgca
     3721 tgccttgttt cttcgggttg attccaatat cctttggcga cactgttact ttgtacccaa
     3781 atttcaccaa cctggtcaaa atcacatggg atcaaggtat caggatctat aattttaact
     3841 tcttgaatgg gattaccact gctgactaac ttgtaactgc ctggactgtt atcgtctgca
     3901 aaatgcacgc gatgatcttg aaattgttct ttggccagag ttaatgtttt gtatgaactt
     3961 cctggtgttc ctcccgtcac taacaaagtg gcctcagcca ggccatagca tggatagaag
     4021 gcttctttac gaaatccaaa ctctttaaat gcctgataaa aatgttccat ggtttcttct
     4081 cgtactggct cagcaccgtt gaaagcagtc acccatgaac ttaaatccag cccttctttt
     4141 ttttcttccc tgattcgttt gacacaataa tcgtaagcga agttagggct tccactgata
     4201 gttgctttgt atttggtaat atgttttaac caggaaagcg ggttttgtaa aaatgagaaa
     4261 ggggacatca tgattgcctg aattccacca tagatggggg tcagaatgca gccaatcaaa
     4321 cccatatcat gatgtggggg cagccagctg aaaataatgg tttcatcatt catatgaaaa
     4381 gaggtaaaaa ttttattcag attatccagt aaattatggt ggcttaccat cactccttta
     4441 gggtgcatgg ttgagccgga agtgtattgc agaaacgcaa tgtcattgct cttaatggag
     4501 gttggttgcc aactactact tctgtttaac tcaatgctct caagcgcaat agcaggaatt
     4561 ttcaggaatt tgggatttgt atttaattcg tctgcggtga attttttgat gtgatccgca
     4621 atcatcagta ctatgaccgg ttttgagttg gtaactatgc gttgtgcctt gtccaataat
     4681 ttttcttggg ctggtgggta aatgggtaca gcaatgcagc ctgcataaag gcagcccaaa
     4741 aatgcctgga taaggggtaa tccaggtgca aacaataaca agaccctatc cccaggtttt
     4801 gctccttctg cttgcaaagt tgccgcaatt gctttggcgt gttgatccag ttgctcataa
     4861 gtcatcgtct cttccaactc tttgttcaga aaagtacagc ttttcttgtt agggctgtgt
     4921 aaagcacgta acctgacgac gtcaaccaga gactggcact gcaaatattc ttttttcact
     4981 ataaaacacc ttaattgcta tatgatgctt tttgatagtt taggacttgt cccgcatagg
     5041 atagaccacc accaacactc atcaggagaa ttttccctga tctgtcaaac aatttttcct
     5101 caactgccct tgctaatgtg tagagaactg atgcgccacc tatattgcct atcgtatcag
     5161 catcccagat tattattttg ttgtttaaat tattttcatg aagtacttgg tctattaatt
     5221 ttcgattgac ctgatggggg attacccatg ttatctcgtt tttttccttt tctgttggca
     5281 ataaatgatc aagcatttgc atgtatctgg aataggctaa ttctttcatt agggtttcat
     5341 cacccaccag atgataacca accgaatcga tttgctcatg agttggcggg aatacccctt
     5401 gagtcgtaaa tgcgttgacg tattgaccat cagtagaaaa aaaactcttt tcaatggtaa
     5461 aagacccctc cttgcttttt tctaaaagta aggaggcagc tccatcagcc aaacctatcc
     5521 aggttttttc attctcaggg tcaatcactt tggaaagtgt ttcagagcaa caaaccaata
     5581 ttttttgata acccaaagcc atcaatgcgt aggcaaaatg caaggtcgat aaagaagtgg
     5641 agcaaccggt ttttaaatcg taagcgggcg cattcaactt caggtttcca agtacagcag
     5701 ccgcttgaga tcccgtaaag cgtttattag ttgtcgagcc taagagaaat gcgtcgacat
     5761 cttttgacat actgtgggtt atcagtttat ctactgcttg catagctagt gattcggaag
     5821 acagttctct tgaattatcg agtgattccc aaggtttgtg gcaccagtat cgagtatgaa
     5881 agccaaattc ttcgcctatt ttttctgcga atttttcgag aaaaccttga gacttatctg
     5941 cagttctcgg aaaattcttt aaaatttcca ggtttgtgat agggcctgat gcaggcagaa
     6001 tccttgatgc gccagtaatt tcaaattgta tgttaggttt taa
//
LOCUS       DACTJE010000180         5888 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.182, whole genome shotgun sequence.
ACCESSION   DACTJE010000180 DACTJE010000000
VERSION     DACTJE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.182"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(200..1432)
                     /locus_tag="I9760_002645"
     CDS             complement(200..1432)
                     /locus_tag="I9760_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HAT5044394.1"
                     /translation="MNRPVVIVDPLSSGIELAPAFKARGIPAIAVTLKPLDWIGFGAN
                     MQTSDFIEIIPDQPNLVEVLAKYDPIAIIPGTEEGVPLAEALAINLTPQFANDPEKSQ
                     NRLHKAMMQKALQEAGVPALKTLNTASENEVEAWIRTNGLIDSPLIIKPPVSAGSDKV
                     FHIPARGEWKKAFNRVLSEPSKITGKVNATVVVQEQAIGTEFAVGTVSANGKHYLAHL
                     IQYNKTSFNDRKTVYDYVEFVPYSKERYGELFDYTQKALDALGIRWGAAHNEIMLTKD
                     GPRLIETGARMCGGPVVGFAREATGSSQADKLVEIYVDGGVSAEEYVFKKTVVPVFLK
                     SPAKGKISNVEAFADLSKLPTFLNEHIWFKNGDLVPQTVDYLTSIGIIGLAGDRDSIL
                     LDYEKIRNMESKLVIQTF"
     gene            complement(1429..2577)
                     /locus_tag="I9760_002646"
     CDS             complement(1429..2577)
                     /locus_tag="I9760_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT5044395.1"
                     /translation="MNKSLIFLAIGMFTVGCNTFLIAGLLPQIGETLEQSVAVTGQGV
                     SLFSLTYLFSAPLFSLILVNQPVKRMIQLALTVFMLGNLITLLSENIVLFLIGRSLAG
                     AGTGIFTPLCISIAIHFTRPSAKGRILSFIWSANSAGVVFGVPAGLYLSSLFHWQLSI
                     ASLIILSLLALIGFSMQNIDIKLPKPSPFGGRLRLLIEPKTLSVIGITCFTALASLGL
                     YSYVTLIQSGSPNSLSMTLLSWGLGGFIGSSLIGVFIDRTGKPRVIMALILVGLMFAL
                     IAIPFTRNLPYLGLIPFFMWGACGWAIVTPQQHILYELHENQGTILAAINSSALGLGS
                     ALGTLLGGLLISSGFRGIYLPFSAATLLFFVLIIQLIVINTSHKVNNI"
     gene            complement(2574..4016)
                     /locus_tag="I9760_002647"
     CDS             complement(2574..4016)
                     /locus_tag="I9760_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAT5044396.1"
                     /translation="MSAQVADVVIVGAGPVGLMCAYLGQLCGINVVIVDKSNCPLEVG
                     RADALNARTLQLLELVDLFDELYPLGKTCNTSSVWADGQFISRQSSWWEELEGCLHKH
                     FLMLGQSYVEKLLDDKLKETAAAVKRSTAIVNIEINEAGCLTTLANGERIQSRYVIGA
                     DGSRSFVRNHFAIPFEIIRPQIIWAVIDGIIDTDFPKVPEIIVFQAETSDVAWIPREG
                     EIDRFYVRMDTKDFTLQEAMDKINHAVRPHSLNFKDIVWFSQFSVKESVAESFFIQDR
                     IFLAGDACHIHSVNGGQGLNTGLADAFNLMWKLHMVMHFGAPKELLHSYEAERKPVAH
                     GVIETSGELVRSTKYSLNGTHAQDYVRIVQKRAGYITGMGIRYGETGLCGSRLFDFEI
                     FNGIAKTRLYSLLDYMKFTLFIFGDCEVELQPPEFVKVIPIYPNQYQKNFWTNNTHYA
                     NQAILVRPDSYIQYAVPLDKIESLFELAQL"
     gene            complement(4013..4849)
                     /locus_tag="I9760_002648"
     CDS             complement(4013..4849)
                     /locus_tag="I9760_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HAT5044397.1"
                     /translation="MNYKVTSLKPFGVLLEPVSEKTKVTDVDIENLRHLFAKNQLVVL
                     RGFCAFQNAEDFSNYCELWGEVSLWPFGKVLELIEQEDPEDHIFDHSYMPLHWDGMYR
                     PQVPEYQIFHCVKAPLPGQGGRTTFSNTILALQFASSEIKELWNKVSGTYQRKMEFYN
                     SKTVSPIITKHPQKDFSVIRYNEPPSVDKGHFVNPPDIEFTGIDQEELDFFHRSLENA
                     LYSPDNFYAHEWQNGDIVIADNFSLLHGREGFVSRSPRHIQRVHVLSNPPFDNPGLES
                     YQ"
     gene            complement(4839..5888)
                     /locus_tag="I9760_002649"
     CDS             complement(4839..5888)
                     /locus_tag="I9760_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyoverdine biosynthesis protein PvcA"
                     /protein_id="HAT5044398.1"
                     /translation="MKNTAFLITKNHYPKDYRLKKSPDSDSAMGVAKRILAEVMIFRR
                     VPETISLCGTGCQKCASPHLPKIISAVNKNEPVTFILPAFPGKSPNPEKVLGHLPDHA
                     ERLSLNFLGTLCQRIKNFYTPGIKIILCSDGRVFSDVVGMNESDVTAYQLELDRLIKE
                     MSLADLSTFNLDYFYKDLHFVQMREELMKSYGQSLDFLKQKIRNGAKPSASPDEQEAN
                     RMYSGITRFLFEDAMHAGQTKSRTAIQKESRFKAYEVIRRSNAWSALIAERFPKAVRL
                     SIHPQTCGSKKLGIRLIGNESWMTPWHGVAVESNKGYVLLRRSEAEALGAKLICSSDG
                     RHSHYQLMAEVSYEL"
BASE COUNT         1795 a         1310 c         1137 g         1646 t
ORIGIN      
        1 ttcgctcatt tgcttcacca aaacttttgg aatttttatc catttttatt tttaaaataa
       61 ataatataag tataggccga tttataatgg ataagctgtt tgtttattag gctaaagaat
      121 tgttcatata aaactcaagt aaccataata cttcaaacca ttgaaaaaat atttcaatgg
      181 cagttcaaca atagaaatcc taaaatgttt ggataaccaa tttcgactcc atatttcgaa
      241 ttttttcgta atctaataaa atggagtcgc ggtctccagc caacccaata attccaatac
      301 tcgttaaata atcaacggtt tgtggaacaa ggtcaccatt tttaaaccaa atatgttcat
      361 taagaaaggt tggtaatttc gataggtcgg cgaaagcttc aacattcgaa attttgcctt
      421 tagcgggtga tttcaaaaaa acaggcacaa ccgttttttt aaatacatat tcttctgctg
      481 aaacaccccc atcgacatag atttccacta atttatcggc ttgactactg cctgtcgcct
      541 ctcgagcaaa gccaaccact ggaccaccac acattctggc gcctgtttct atgaggcgag
      601 ggccatcttt agtgagcatg atttcattat gagcagctcc ccagcgaatt cccaaagcgt
      661 ccaatgcttt ttgcgtataa tcaaataatt caccatatct ttctttgcta taaggcacaa
      721 attccacata gtcgtaaact gttttgcgat cattaaagga tgttttattg tattggatta
      781 aatgcgctaa atagtgtttt ccattagcac tcactgtgcc tacagcaaat tccgttccta
      841 tggcctgctc ttgcacaact acagttgcat tgactttccc ggtaatttta gatggctccg
      901 ataaaacccg gttgaatgct tttttccact cccctctggc tggaatatga aagaccttat
      961 cactgccagc agatactggg ggtttaataa taagtggaga atcaattaat ccgtttgtcc
     1021 tgatccaggc ttcaacctca ttttcagatg ccgtattgag cgtcttaagg gcaggaactc
     1081 cagcttcctg cagtgctttt tgcatcatag ccttgtgcaa tctattctgt gatttttcag
     1141 gatcattggc aaattgaggc gtcaagttta tcgccaacgc ttccgcgaga gggactcctt
     1201 cctcagtacc aggaataatg gcaatagggt catattttgc aagtacctcc acaagatttg
     1261 gttggtctgg aataatctcg ataaaatcag aggtttgcat gtttgctcca aatccaatcc
     1321 aatccagagg tttaagcgtg actgcaatag caggtatccc tcgagcctta aaggcagggg
     1381 ctaactctat tcctgatgat aatggatcga caatgacaac gggcctgttc atatgttatt
     1441 taccttatgt gaagtgttaa ttactatcag ctgaattatc aatacgaaaa acaataaagt
     1501 ggcagcagaa aaaggaagat agattcccct gaatccagag gaaatcaata agccgccaag
     1561 caacgttccc aaagctgacc ccaagcccaa ggccgatgaa ttgatagcgg caaggatagt
     1621 tccctgattt tcatgtaatt catataaaat gtgttgctgg ggagtcacta tagcccatcc
     1681 gcaagcaccc cacataaaaa aagggattaa tcccaggtaa ggcagattcc tggtgaatgg
     1741 tatggcaatc agagcaaaca tgaggccaac caaaattaag gccataataa cccttggttt
     1801 acccgttcga tcgataaaca ccccaatcag tgagcttcct ataaatccac ccagtcccca
     1861 actcaatagc gtcatactga gcgaattagg ggatcctgat tgaatcaggg tgacatacga
     1921 gtataatccc aaacttgcca aagcggtaaa gcaagtaatt ccaattaccg atagcgtttt
     1981 tggctcgatc agaagacgaa gcctgcctcc aaatggcgaa ggtttgggta gttttatatc
     2041 aatgttttgc attgaaaaac caatcaatgc aagcaaactt aaaataataa gactggcaat
     2101 cgataactgc caatgaaaca aggaggataa gtaaagtccg gcaggaacgc caaatactac
     2161 acccgcactg ttagcactcc agataaaact taaaattcgt cctttggcag atggcctggt
     2221 aaaatgaatg gcaatgctga tacataacgg cgtaaaaatc ccggttcctg ctcccgccag
     2281 agatcttcca attaaaaaca gcacgatatt ttcagaaagt agcgttatta aattgccaag
     2341 catgaagaca gtaagcgcaa gctgaatcat acgctttacc ggctgattaa ccaaaatcag
     2401 agaaaaaagt ggcgctgaaa aaagataagt caaactgaat agactcactc cttgcccagt
     2461 taccgcaacc gactgctcga gcgtttcgcc tatttgagga agcagaccag caattagaaa
     2521 tgtattgcaa cctactgtaa acatgcctat tgctaaaaaa atcaggcttt tattcataac
     2581 tgcgccaact caaataagga ttctatttta tccagcggca cagcgtattg aatgtaggag
     2641 tcaggcctta ctaaaatggc ctggtttgca tagtgtgtgt tattggtcca aaaatttttc
     2701 tggtattggt ttggatatat tggaatgact ttgacaaact ccggaggctg taactcgact
     2761 tcacagtcac caaagataaa taaagtgaat ttcatataat ccagaagaga gtaaagccgt
     2821 gttttggcaa tgccattaaa aatttcaaaa tcaaagagtc gagaaccaca caaacctgtt
     2881 tctccataac gaatccccat ccccgtgata taacccgcgc gcttttgcac aatcctgaca
     2941 tagtcttggg catgggttcc attaagcgaa tacttggtcg agcgcacaag ctcaccggaa
     3001 gtctctatca caccgtgagc tactggctta cgctcagctt cataactgtg taaaagttct
     3061 ttgggagcgc cgaaatgcat gaccatgtgc aatttccaca tgagattaaa cgcatctgca
     3121 agaccagtgt taagaccttg cccaccgttt accgaatgaa tatggcaggc gtcaccggca
     3181 agaaaaatgc gatcctgaat gaagaaactc tcagcgacag attctttaac tgaaaattgt
     3241 gaaaaccaaa caatgtcctt aaaatttaag gaatggggcc gcacggcatg attaattttg
     3301 tccattgctt cctgcaaggt aaagtcttta gtatccattc ttacgtaaaa cctgtcgatt
     3361 tccccttccc ttggaatcca ggccacatcc gaagtttctg cctgaaacac aatgatttca
     3421 ggaacttttg gaaaatcggt gtcaataata ccatcgatta ccgcccatat aatttgtggc
     3481 cgtatgatct caaaaggtat tgcaaaatga ttgcggacaa aggaacgaga tccatcggct
     3541 ccgatgacgt agcgtgactg aatgcgctct ccattggcaa gagtcgtaag acatcctgct
     3601 tcattaatct caatgttgac aatcgcagtt gaacgtttta ctgctgccgc agtttccttc
     3661 aacttgtcat ctaacaattt ttctacatag gattggccaa gcataaggaa atgtttatgc
     3721 aaacaaccct ccaattcttc ccaccatgac gattgtcggg aaatgaattg cccgtctgcc
     3781 catacagaac tggtattgca ggtctttcct aaaggataaa gctcatcaaa taaatcaact
     3841 aattcaagaa gttgtaaagt gcgcgcgtta agggcatctg ctctaccgac ctctaaagga
     3901 caattagact tatcgacaat cacaacattg atgccacata gttgtcccag ataagcacac
     3961 atgagcccta ctggacccgc accgacaatt acaacgtctg caacttgtgc gctcattgat
     4021 aagactccag acccgggtta tcaaaaggcg gattactcaa aacatgaacg cgttggatat
     4081 gacgaggcga cctggagaca aacccttctc tgccatgtaa taaagaaaaa ttatctgcaa
     4141 tgacgatgtc cccgttttgc cactcatgag catagaaatt atccggtgag taaagagcgt
     4201 tttctaaact tcggtggaag aagtccaact cttcctgatc aataccagta aattcaatat
     4261 cgggtggatt tacaaaatga cctttatcta cagagggagg ttcattgtag cgaatgactg
     4321 aaaaatcctt ttgaggatgc ttcgtgataa ttggtgatac cgtcttgctg ttataaaact
     4381 ccattttcct ttggtaggta ccactcacct tattccataa ttctttgatt tctgaagagg
     4441 caaactgtaa cgctaaaatt gtgtttgaaa aagtagtcct tcctccttgt ccaggcaaag
     4501 gcgctttcac acaatgaaaa atctgatatt caggcacttg gggtcgatac ataccatccc
     4561 aatgcaaggg catgtaacta tgatcaaaaa tatgatcttc cggatcctct tgctcaatta
     4621 actcaagtac cttgccaaat ggccaaaggc tgacttctcc ccacaactcg caataattag
     4681 aaaaatcctc tgcattttga aaggcgcaaa agcctcttaa tacaacgagt tgattctttg
     4741 caaataaatg gcgcaagttc tctatatcga catcagtcac cttcgttttt tcgctcacag
     4801 gctccaagag tactccaaat ggttttagag aagtcacttt ataattcata tgacacctcc
     4861 gccattaatt gataatgact gtgccgacca tcagaggaac aaattaattt tgcacccaaa
     4921 gcctcagctt ccgagcgcct cagcaagaca taccccttat tgctttcaac agcaacacca
     4981 tgccaagggg tcatccagct ttcattccct atcaaccgaa tccctaattt ttttgatccg
     5041 caagtttgtg gatgaataga caaccgaact gccttgggaa agcgttcggc aatgagcgca
     5101 ctccaggcgt tgcttcttct tataacctca taggctttaa aacgagattc tttttggata
     5161 gcagtacgac ttttggtttg acctgcatgc atggcatctt caaacaaaaa acgcgtaatt
     5221 ccgctataca tgcgattggc ttcttgttca tcggggcttg ctgagggttt tgcaccatta
     5281 cggatctttt gtttaaggaa gtccagggac tgtccatagc ttttcatgag ttcctcacgc
     5341 atttggacaa aatgaagatc tttataaaaa taatcaagat taaaagtgga cagatctgca
     5401 agggacattt cttttataag tctgtccagc tcgagctggt aggctgtgac atcactttca
     5461 ttcattccaa ccacatcgct aaaaaccctt ccatcggaac acagaataat tttaatcccc
     5521 ggggtataaa aattttttat tcgctgacaa agtgttccca aaaaattaag agaaaggcgt
     5581 tcagcatgat ctggcagatg acccagaact ttttcagggt tgggagattt tcccgggaag
     5641 gcaggcaata taaaagtaac cggttcattc ttattaactg ccgagatgat ttttggcaaa
     5701 tgaggtgaag cgcacttttg gcatccggtg ccgcataaac taatggtttc cgggacacgg
     5761 cgaaaaatca ttacttcagc taatattctt ttggctactc ccatagcaga gtcactatcg
     5821 ggtgattttt tcagtcgata atctttaggg taatgattct ttgtaattaa aaacgctgta
     5881 ttcttcat
//
LOCUS       DACTJE010000181         5848 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.183, whole genome shotgun sequence.
ACCESSION   DACTJE010000181 DACTJE010000000
VERSION     DACTJE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.183"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            90..2783
                     /locus_tag="I9760_002650"
     CDS             90..2783
                     /locus_tag="I9760_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAT5044399.1"
                     /translation="MVLQWHEKTLQDIVSQFKADLVLGLTEAEAVRRLKEVGPNLLDK
                     QKRTSPLVIFLQQFSNIIIWVLLGAVVVSFLLGEKADAIAIFAIVILNAIIGFALEYR
                     ADRAILALQQMAAPKATVLRDGYAKLIAASDIVPGDVILFESGDLIAADARLFEFSAL
                     KVNEAPLTGESLPVAKNLDLCTKDTSLADRKNMVFMGTAVVNGTGRALVIATGMQTEM
                     GRIAQLLGEASRDKTPLQKKLNQVGSRLLWVCFFIVMAIFGLGLLRNIPIFNLFMSSV
                     SLAVAAIPEGLPAVVTVALALGVQRMVRRAVLVRRLSAVETLGYLQVICTDKTGTLTV
                     GEMTARKFVTASDVYSIYGEGYNLAGKFSLQGQEINVSEDKLLQASLQAMVSCNNAEF
                     RQQNGQMTVAGDPTEVALLVAAAKGGFWRSELQISNPRIKELPFSSERKRMTVVCQYD
                     NDELMVYVKGAPEIVLERCTHILTKTGIKKLTLNDKARMRQSCEFMASEALRILAFAQ
                     RPLDTSWLDKEIEEIESNLVFLGLIGLQDPPHSSAKESVSRCKKAGIKLVMITGDHPV
                     TARAIAQELGILSEGDQLLTGNELENISEEEFNSCVKDIAVYARVTAEHKLKIVRAWK
                     KQNRVVAMTGDGVNDAPALKEASIGIAMGKTGASVTKEASDIIVMDNNFTSIVAGIEE
                     GRTIYDNIIKTLIYLLAGNSAELLVVFVALLIGWPLPLLPIQLLWINLVTDGLPAIGL
                     ATDRSEPGILNRPARTIQKSMMDGTFLKRVSFIGCVTAVVTLIVFAYEYLAYKDLTQA
                     QDAAFSILVTARLLIAFGTRSDTKTIFQLGFFSNLHLFIIISISFSLQVLIHHIPVLR
                     DLFGIGQISFTQCISWILIGTLPLLVIEAQKWLRKAPGETF"
     gene            2770..4026
                     /locus_tag="I9760_002651"
     CDS             2770..4026
                     /locus_tag="I9760_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAT5044400.1"
                     /translation="MRHFSVRKIIWIVLLSFCFTESFIYAKPNPGLSQLIEEAKQNNP
                     QIRAARDRLLAAIHVIPQAKALPDPKVNAGYINMSENIPMDVDPRREQMLGGQQEIPF
                     LGKLVVRGRIATLEAKRAEAEYQATCFAVIAELKQLYYDLYFVNKSLEIVQRNQELLH
                     EMEKSAEANYSVGKTPQQDIYRAQTEISRLLMRLVILKQQRESLQADINRLLNRSLEI
                     TVNTPAILSLTPMSHHLDYFYSLIKQRAPQLIMQRRNVQKGRQSIRLSKMEYFPDVEI
                     EGGRLHDTGMHTKGYQVLLKATVPLYFMQKQNNAVRESIARYSADMEDLQTTYRALSF
                     QVKNAYLLAERSAKLIHLIQHTIIPQATLTFTSSQANYGVGKVDFLTMLNNLLTLQEN
                     ELEWHGELAEHEKAITQIEAITGTYL"
     gene            4023..5159
                     /locus_tag="I9760_002652"
     CDS             4023..5159
                     /locus_tag="I9760_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAT5044401.1"
                     /translation="MNNKVMIKSMFVLSLLSLLPLITYAQNTTDMHGKMDMKQPTQKN
                     EIVIDPVRLQAIGITSEPVRRQNIEKTIRTVGRVAVDERRVAHIHVRFDGWIEKLFIN
                     FTGEKVKAGQALFTVYSPDLVSTQQEYLLALNAQKILSQNTTSIAAQGAKDAFQAAHQ
                     RLLLWGISEKDIEQLKHTGKITRTMTIHSPIKGTVLKKMAFIGMRIEPGDELYTIADL
                     SHLWVLGDIYEYELPYIRLGQTADLTITYLPNELFKAKLDFIYPTIDLKTRTVKVRFE
                     VNNQKEQLKPGMYVNLELKIPFGERLVVPKNAVLLTGERAVVFIYHGKGKIEWRDVTL
                     GVRAGDLLEIVQGVKEGDEIITSANFLIDSESQLKAAMGGMQHK"
     gene            5198..>5848
                     /locus_tag="I9760_002653"
     CDS             5198..>5848
                     /locus_tag="I9760_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAT5044402.1"
                     /translation="MVERIIEFCVRNRFIVLLFIFGFSFMGYMALKNTRMDALPDISD
                     TQVIVYTTWMGRSPDLIEDQVTYPIVTALLSAPKVVAVRGFSDFGFSYVYIIFEDGTD
                     IYWARSRVLEYMSQLAGKLPEGVTPQLGPDATPVGWIYQYALVDENGKHNLADLRTFQ
                     DWYLRYWLRSVPGVSEVASVGGFVKQYQINLDPVKVLAYNLSVPDIIEKIRMSNNDT"
BASE COUNT         1689 a         1084 c         1316 g         1759 t
ORIGIN      
        1 tttagaatgt tctcttgtat tttaaggtaa ttatgtagaa ctttttacct cattaaatta
       61 agggaaagct ggtccaaagg atggatgcaa tggtactgca atggcacgaa aaaacgcttc
      121 aggatattgt ttctcagttt aaagcagatc tggttttggg gttaaccgag gcagaagcgg
      181 tacggcgatt aaaagaagta gggccaaacc tactcgacaa gcaaaaaaga acgtcacctt
      241 tggttatttt tttgcaacaa tttagcaata taatcatctg ggtgctttta ggtgctgttg
      301 ttgtttcttt tttactcggc gaaaaagctg atgccattgc catttttgcc attgtcattt
      361 taaatgccat cattggcttt gctttagaat atcgtgccga tcgggcgata ttggctctgc
      421 agcaaatggc tgctcctaaa gcaaccgtgc ttcgagatgg ctatgcgaag ctgattgcgg
      481 cttctgatat cgtaccaggt gatgttattc tttttgaaag cggagattta attgccgctg
      541 atgctcgtct attcgaattt tcagccctta aagtcaatga agcgccactc acaggagaat
      601 ccctcccggt tgctaaaaat ctggatcttt gtacgaaaga cacctctcta gccgatcgta
      661 aaaatatggt gtttatggga acagctgttg tcaatggaac gggacgtgct cttgtcatag
      721 caaccggcat gcaaactgaa atgggtcgta ttgcccagtt actaggtgaa gcatctcgtg
      781 ataaaacccc tttgcaaaaa aaacttaatc aagtgggatc tcgtttatta tgggtttgct
      841 ttttcattgt tatggctatt tttggcttag gcctcctgcg taatattcct atcttcaatc
      901 tgtttatgag ttcagttagt cttgctgttg ctgcgattcc tgaggggtta cctgctgtgg
      961 tcacagtggc tttagctctg ggtgtgcaac gtatggtgcg gcgtgctgtg ctggtaagac
     1021 gattatcggc agtagaaaca cttggttatt tacaagtcat ttgcactgat aaaacaggaa
     1081 ctttaactgt gggtgagatg acagcacgta aatttgtgac agcaagcgat gtatatagta
     1141 tttatggaga aggctataat ctcgcaggaa aattttcact tcaaggccaa gagattaatg
     1201 tttcagaaga caagttattg caggcctcat tacaggctat ggtatcctgc aataatgctg
     1261 aatttagaca acaaaatggg caaatgacag tagccgggga tccgactgag gtggctcttt
     1321 tggtcgcagc agctaaagga ggtttttggc gaagtgaact acaaatttct aatcctcgca
     1381 ttaaggagtt gccttttagt tccgaacgca aacgaatgac agtggtttgt caatatgaca
     1441 atgatgagct catggtttac gtaaaagggg ctccagaaat tgttttagaa cgctgcactc
     1501 atattttaac aaaaactggt attaaaaaat taacgctgaa tgataaagcg cgtatgaggc
     1561 aatcatgtga attcatggct agtgaggcat taaggatttt ggcattcgca caacgtccat
     1621 tggacacgtc atggctggat aaagaaattg aggagattga aagcaatctc gtttttttag
     1681 gtctgattgg actgcaagac ccaccgcatt ccagtgcaaa ggaatctgtt tcccgctgta
     1741 aaaaggcggg tatcaaactg gtgatgatta caggagacca tcctgttaca gcgagagcca
     1801 ttgctcaaga acttgggata ttaagcgaag gtgatcaact ccttactggc aatgagcttg
     1861 aaaacatatc agaggaagag tttaactctt gcgtcaaaga cattgcagtc tatgctcgtg
     1921 tgactgcgga gcataaatta aaaatcgtac gtgcttggaa aaaacaaaat agagtagttg
     1981 cgatgacagg tgatggtgtg aatgatgcgc ctgctctaaa agaggcttct attggtattg
     2041 cgatgggaaa aacaggagct tctgttacca aggaagcttc agacattatt gtcatggata
     2101 acaacttcac atcaattgta gcagggattg aagaaggtcg aacgatttac gataatatta
     2161 tcaaaacact catttattta ttagcaggta acagtgctga attgctggtt gtttttgtag
     2221 cacttttaat cggatggccc ttgcctttgc tccctattca attactatgg attaacttag
     2281 tgactgacgg tttgccagcc attggtttag caactgatag gtcagaacca ggaattttga
     2341 atcgcccagc cagaaccatt caaaaatcca tgatggatgg tacgtttctc aaacgagtga
     2401 gttttattgg ttgtgtaaca gctgtagtaa ctttaattgt ttttgcttat gagtatttag
     2461 cttataagga tttaactcaa gcgcaagatg cagcattttc catattagtg acagcaagac
     2521 ttttgatagc atttggaact cgtagtgata ctaagactat atttcagctc ggttttttct
     2581 ctaatctgca tttatttata attattagca ttagtttttc actacaggtc ttgattcatc
     2641 atattcccgt actacgagat ttgtttggta ttgggcaaat ctcatttact caatgtattt
     2701 cctggatctt gataggcacg ttgcctttgt tagtaattga agcccaaaaa tggctaagga
     2761 aagcacctgg tgagacattt tagtgttaga aaaattattt ggattgttct cctgagtttc
     2821 tgctttacgg agtcattcat ttatgcaaag cctaatcctg gtttatcgca gttaattgag
     2881 gaagctaagc aaaacaatcc ccaaattcgt gcagcacgcg atcgtttact tgccgcaatt
     2941 catgtgatcc cacaagcgaa agcgttgcct gatcccaagg tgaatgctgg ctatatcaat
     3001 atgtcagaga acattcccat ggatgtagat ccaagacgtg aacaaatgct gggtggccaa
     3061 caagaaattc cctttcttgg caagcttgtt gtacgaggaa gaatagcaac gcttgaggct
     3121 aaacgggcag aagcggaata tcaagccacg tgttttgctg tcattgcgga acttaaacag
     3181 ctttattatg atctttactt tgtcaataaa tctcttgaaa tcgtacaaag aaaccaagag
     3241 cttctccatg aaatggaaaa aagtgccgaa gctaattaca gtgtcggaaa aacacctcaa
     3301 caagatattt atcgcgctca aaccgaaatt tctcgtcttc tgatgcgctt ggttatctta
     3361 aagcagcaac gggaatcctt acaggctgac attaatcgtc ttttaaatcg ttccttagaa
     3421 atcacggtca atactccagc catcctttct ctgacaccaa tgagtcatca tttggattat
     3481 ttttattcac tgattaagca gcgagcacct caattaataa tgcaacggcg aaatgttcaa
     3541 aaaggacgtc aatccatccg tttaagtaaa atggagtatt tccccgacgt tgaaatcgaa
     3601 ggtggtcggc ttcacgatac gggaatgcac accaaaggtt atcaagtgtt gttaaaagca
     3661 accgttccgc tttatttcat gcaaaagcaa aataatgctg tgcgcgaatc aattgctcgt
     3721 tacagtgctg atatggagga tttgcagacc acttatcgcg cgctttcttt tcaagtgaaa
     3781 aatgcctatt tattagcaga gcgctctgcc aagcttattc atctgattca acatactatt
     3841 attccgcaag ccactttaac gtttacttct tctcaggcta attacggggt tggtaaggtg
     3901 gattttttaa ccatgcttaa taatttgttg accctgcaag aaaacgaatt ggagtggcac
     3961 ggtgagcttg ctgagcatga gaaagcgatt acgcaaatcg aagcaattac agggacttat
     4021 ctatgaataa taaggtgatg ataaagagta tgtttgtttt aagtttactg tctttgctgc
     4081 cattaataac ctatgcgcaa aatacaaccg atatgcatgg taagatggac atgaaacagc
     4141 ctacacaaaa aaatgaaatt gtaattgacc cagtccgctt acaagccatt ggcatcacct
     4201 cagagccagt acgccgtcaa aacattgaaa aaacaattcg cacggtagga cgggttgcgg
     4261 tcgatgagcg tcgggttgcc catattcatg tgcgctttga tggttggata gagaaattat
     4321 ttatcaattt taccggtgaa aaagtaaagg cagggcaagc cctttttaca gtgtatagcc
     4381 ctgacttggt ttccacccaa caagaatatt tacttgctct caatgcacaa aaaattttga
     4441 gtcaaaatac gacatccatt gccgcccaag gtgcgaaaga tgcttttcaa gcagctcacc
     4501 aaagattatt gttgtgggga atttctgaaa aggatataga gcaattaaaa catactggaa
     4561 aaataacaag gaccatgacc attcattcac cgattaaagg aacggtgctt aaaaaaatgg
     4621 cgtttatcgg tatgcgcata gagccgggtg atgagttgta cactattgct gatttatcgc
     4681 atttgtgggt tttaggggat atttacgagt acgagcttcc ttatatccgt cttggtcaaa
     4741 cggctgattt gacgatcacc tatttaccca atgaattatt taaagccaag cttgatttta
     4801 tttatcccac tattgaccta aaaacacgta cagtcaaggt gcgttttgaa gtgaacaatc
     4861 aaaaagaaca attaaagcct ggcatgtacg tcaatttgga actcaaaatc ccttttggtg
     4921 aacgtcttgt agtgcctaaa aatgccgttt tactcacggg tgaacgagct gtggtcttta
     4981 tttatcacgg caagggtaaa attgaatggc gagacgttac tcttggggtg cgagccggcg
     5041 atttgcttga aatagtccag ggggttaagg aaggagatga gattatcacc tcggcaaact
     5101 ttttgattga ctcagaaagt cagttgaaag cggccatggg aggcatgcag cataaataat
     5161 aaaagggcat gatttgcctt tggaacaaag ggattttatg gttgaacgaa ttattgaatt
     5221 ttgtgtgcgc aatcgtttta ttgtgttgct gttcatattc gggttttctt ttatgggcta
     5281 catggccttg aagaataccc gcatggatgc attacctgat atttcagaca cgcaggtgat
     5341 tgtttatact acttggatgg gacgctcccc ggaccttata gaagatcaag tgacttatcc
     5401 tattgtgaca gcactgctgt cggcaccgaa agtggttgct gtgcgaggat tttctgattt
     5461 cggattttct tatgtttata tcatttttga agacggtact gatatttatt gggcgcgttc
     5521 acgagtcttg gagtacatga gccaactggc aggaaagttg cctgaagggg tgacgccgca
     5581 attaggtcct gatgctactc ctgtaggttg gatttatcaa tatgccttgg tagatgaaaa
     5641 cgggaagcat aatctggcag acttacgtac atttcaggat tggtatttac gttattggtt
     5701 aagaagcgtt cctggtgtgt ctgaagtagc tagcgtgggt ggttttgtca agcaatatca
     5761 aatcaattta gatccggtca aggttcttgc ctacaatcta tctgttcctg acatcatcga
     5821 aaaaatacgt atgagtaata atgatacc
//
LOCUS       DACTJE010000182         5848 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.184, whole genome shotgun sequence.
ACCESSION   DACTJE010000182 DACTJE010000000
VERSION     DACTJE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.184"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1083
                     /locus_tag="I9760_002654"
     CDS             <1..1083
                     /locus_tag="I9760_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT5044403.1"
                     /translation="FFSKVSHPYVQQLLTSLPSFAKRNEKLSIIAGTVPSLESMPSGC
                     RFHTRCIYAFERCHHEEPQLQEVEQRILRCHLYPDIKELPLLDKNKISWVPSVEKEEI
                     VFSVDDLSVHFIHKKGFLSRKKTIFKAVDGLSFTLSRGKTLALVGESGCGKTTASRAL
                     LRLLPAAGGEIRYKDSDVLSMTGSALRRYRKKVQIIFQDPFSSMNPRMTVGEIIAEGM
                     HAQGMKPSYIRKQQKSLIEQVNLPITSLHRYPHQFSGGQRQRICIARALATEPDILIC
                     DEPTSALDISVQAQILNLLKELQQQRGISYLFITHNMGVVSYIADDVLVMKDGLAVEY
                     GACEKVLRQPEHPYTRQLLNAVLDIE"
     gene            1172..5455
                     /locus_tag="I9760_002655"
     CDS             1172..5455
                     /locus_tag="I9760_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044404.1"
                     /translation="MKITRNQLNFKGVKEFGGCLTRNDEQIEFINKEIKLLKELIEFN
                     ILKGIDENKSQSLFDAVIEAVEKAGLDVEHELLIQFIQSTSAIIDSKSTDLPELISLL
                     TVLIKSSKKYDKSELTRYCRLIDKLEKTDEKSLEQWIKILTILGRNVESVNDDLLFNV
                     QSRLEIHSVLLKDISSLFDYPPYPALDVFSEKLREYSRELKLYIDSFDRDPAGSRAPK
                     VNQEGILVKSREQILNEQFDTSQIQEVIAKIESLVDGSGLSCKGQYELAQQVVFINAI
                     GRDFPLKIANETYTDLTQLGRNKLQELSDFLIMEMRRLDISKEDKLRTQLKLLAVMRE
                     QYFRSTGIFFDTTQMISLLLSLHHQEQNVLMGVSCNENLSAPPAAILAAMQWVLADGG
                     TVDLCFANHGLIAQDYLEKGDRDFFTCLGIPSSVVEANSESGTYRVGGINYSTLSDLT
                     SYHSHAVIEDEELSMDRCGYPVSSYLILNHIDFSNVDDRTLSHFSSLYDYYKGKGRVI
                     RITEIEETVGEVVGQISRFDMDVGYRVFPARKNLQEELNTQSLAERRREEAVKQYFIQ
                     SVSNIQQAVLNQLEEWQAFLHLIYPKSEWRQLDNELFAQRNELIDSLKKQWTKCLEDS
                     DPKKMYPNPYVRRDVFQRLQTRELEKAIQAYEKSVSSIWNLNRDQLKAKTENRFASDS
                     VNALRCKYLEEVDLSEQIKWSKLSARLKKKEATHEKKRSHRHLESAVEVTGAMLSYHD
                     GDLEPYRLAFVHSQLKLWARDISKEINNSSLPACVKQGLIERANNADNFLALELLLID
                     YESRWLDKEKLSEKFRMQPIISDMLRVYKQAGFEEHKELKILKETYLDSTATQIIHHL
                     DKTLAWAKPENRGPWYWIERSAVKKAANEILVLANGVKQARDSLSKQIAIKKLYRGLC
                     KHQAQLEDVWIFSFDHKNTRDLINQTLNTMDSLTVLGRDEGGLDINFIQECKEEAQYY
                     LLGEKFSSVLEDFEKNNSQILTAHSEWQKVKRQLQQKQNESRKVYTLNEMYYVLCKKI
                     NELSISYSLLREPLVQLRGMIRTLWNSFQEKHQDIIDESKYFDFKARKIKEELEGIEG
                     YSVFNVKVKKGYTGFNEYVELIIEGEGTSALFEGFMQYKSRLTELKEEYRQLEFSLKQ
                     NRLELLNLNRIHAKHLPFLKDRIKGNPEVELFPEQCRSKVKEILTLKEWINGQIPENL
                     SQFPENMQNHFLDRNTLKNMDRNTMTMEQIDKIKDEQLKADLTELYNKMHKVDEEQSQ
                     SWFSTVTRRIVGLIPSMFRQEETEDDWRYQFNELQGRTDSILQSLLTKKIMTKYDALV
                     NEMVLIQSNLAQQMDSGNKKIQFLSDKILEEERKTNVIIKRFDNLNDFYDFEAKLKRV
                     KTAPPIAPVNEVQIESKNRILSCGEGAFSNPEEEFQTDSVMQPPMVFSI"
BASE COUNT         1939 a          946 c         1250 g         1713 t
ORIGIN      
        1 tttttttcaa aagtgagtca tccttatgtc cagcaacttt tgacatcctt accttcattt
       61 gccaaaagaa atgaaaaact gtctatcatt gcaggcacgg taccttcttt ggagtctatg
      121 ccttcaggtt gtcgtttcca tacacgctgt atttatgctt ttgaacgttg tcatcatgag
      181 gagcctcaat tacaagaagt tgagcagcgt atcttgagat gtcatttgta tcctgatatc
      241 aaagaattgc cattactgga taaaaataaa atcagttggg ttccttccgt tgaaaaagaa
      301 gagattgtgt tctctgtaga tgatttatca gttcatttta tccataagaa aggatttttg
      361 agtcgtaaga aaaccatttt caaagccgtt gatggtttgt cgttcactct ctccagaggt
      421 aaaacattag ctttggttgg agagtcgggc tgtgggaaaa caactgcaag ccgtgctctg
      481 ttgaggttat tgccagcggc cgggggggaa atcagatata aggatagtga tgtactgtct
      541 atgacaggaa gcgctttaag aagatacaga aaaaaagtac agattatttt tcaagatcct
      601 ttttcttcta tgaatccacg catgacagtt ggagagatca tcgcagaagg gatgcatgca
      661 cagggaatga agccttctta catcaggaaa caacaaaaat ccctaattga gcaagtgaac
      721 ttaccaatta ccagtctgca tcgataccct catcagtttt ccggtggaca aagacaacgg
      781 atctgcattg cgagagcttt ggcgacagaa cctgatattt taatctgcga tgagccgacc
      841 agtgctcttg atatctctgt acaggcacaa attcttaatt tattaaagga attgcaacag
      901 caaagaggta tttcctattt gtttataacc cataacatgg gggtcgtctc ttatatcgct
      961 gatgatgtat tagtcatgaa agacggcttg gctgttgaat atggagcgtg cgagaaggta
     1021 cttcggcaac cagaacaccc ttataccagg cagctgttaa atgctgtatt ggacatcgag
     1081 taatatttca aattttcttt tacatttatc tgctacattt tatgcagctt atatataatg
     1141 agggcattct atttttcagg gaattcaggg catgaaaatc actcgaaatc aattaaattt
     1201 caaaggtgta aaggaatttg gtggttgtct aacaagaaat gatgaacaaa ttgaattcat
     1261 taacaaagag atcaagctgt tgaaggaatt aatcgagttc aatattctga agggaatcga
     1321 tgaaaacaaa tcacaatcct tatttgatgc tgtgattgag gcagtggaaa aggctggctt
     1381 ggatgtcgag catgaactgc tcatacaatt tattcaatcc acatcagcga ttattgactc
     1441 caaatcgaca gaccttcctg agttgatttc cttattaacg gtactcatta aatcaagtaa
     1501 gaagtatgac aaatcagaat tgaccagata ttgccgtctt atcgataagc ttgaaaaaac
     1561 ggatgagaaa tccttagaac aatggataaa aatactgact atacttggaa gaaatgttga
     1621 gagtgtaaat gatgatttat tattcaatgt tcagtctcgc cttgagatcc atagtgtttt
     1681 actaaaagat atttcttctt tatttgatta tcctccttat cccgcattgg atgttttttc
     1741 cgagaaattg cgtgaatact caagagagtt aaaattatat attgattcat ttgacagaga
     1801 tcctgccgga tccagggctc ccaaagtgaa tcaagaaggt attcttgtta aatcgagaga
     1861 acaaattctt aatgagcaat ttgatacaag tcaaattcaa gaggttattg ccaagattga
     1921 aagtttggtt gatggatcgg gtttatcgtg taaagggcaa tatgaattgg ctcaacaagt
     1981 tgtatttatc aatgcaatag gcagggattt tcctctaaaa atagcgaatg aaacgtatac
     2041 tgatttaacc caattaggca ggaataagtt gcaggagttg tctgattttt taattatgga
     2101 gatgagaaga ctcgatatta gcaaagagga taaactgcga actcaactga agttactcgc
     2161 agtgatgcga gagcagtatt ttcgcagtac cggcatattc tttgatacta cacaaatgat
     2221 ttctctttta ttgtctttac atcatcaaga acaaaatgtg ttaatgggag tcagttgtaa
     2281 tgaaaacctg agcgctccgc ctgcagcaat tcttgctgcc atgcaatggg tgcttgctga
     2341 tggtggtacc gttgacctgt gttttgcaaa tcacggatta atagctcagg attatcttga
     2401 gaagggtgat agagatttct ttacctgttt gggtatacct tcctctgtgg tagaggcaaa
     2461 ttctgagtct ggtacttacc gggttggggg tattaattac tcaacacttt ctgatttgac
     2521 ctcttatcac tcgcacgcag tgatagagga tgaggaactg tctatggata gatgcggata
     2581 tcctgtttca agctacctta ttcttaatca tattgatttt tctaatgtag acgatagaac
     2641 tttatctcat ttttcttcac tgtatgatta ttataaaggg aaggggcgtg tcatcaggat
     2701 aaccgaaata gaggaaaccg ttggtgaagt ggttgggcaa atcagccggt tcgatatgga
     2761 tgtggggtat cgggttttcc ctgccaggaa gaatttgcag gaagaattaa atacgcaaag
     2821 tttggctgaa agacgaagag aagaagcagt taaacagtat ttcatacaat ctgtttctaa
     2881 tattcaacaa gcggtattaa atcaattgga agaatggcaa gcatttttgc atttaattta
     2941 ccccaagtca gaatggagac agctggataa tgaattattt gcgcaaagaa atgagttgat
     3001 tgattcgcta aagaagcaat ggacaaagtg tttggaagat tctgatccta aaaaaatgta
     3061 tccaaacccc tatgtccgta gggatgtttt tcagcgctta caaaccagag aattggaaaa
     3121 ggcaattcaa gcctatgaaa aatctgtttc cagcatttgg aacctaaatc gagatcagtt
     3181 gaaagcaaaa acagaaaata gattcgccag tgactcagtc aatgcattgc gttgtaaata
     3241 tttggaagag gtcgatttaa gtgaacaaat aaagtggagt aaattatcag ctcgtttaaa
     3301 gaaaaaagaa gcaactcatg agaaaaagcg cagtcatcgt caccttgagt cagcagtaga
     3361 agtaactggt gcgatgttaa gctaccatga tggtgattta gagccctatc gattagcttt
     3421 tgtgcacagc caactgaaat tgtgggccag ggatattagc aaagaaatca ataactcctc
     3481 attacctgcc tgtgttaaac aaggcctaat agagagagct aataatgcgg ataactttct
     3541 tgctttggag ttgttattaa ttgattatga aagcagatgg ttagacaaag agaaattgag
     3601 cgaaaaattc cgcatgcaac caattatcag tgatatgctg agagtgtata agcaagcagg
     3661 ttttgaagaa cataaagaac ttaagatttt aaaagaaacc tatctggata gtacagctac
     3721 acaaatcatt catcatcttg acaagacatt ggcttgggct aagcctgaaa acagaggacc
     3781 ttggtattgg attgagcggt cagcagtaaa aaaagcggcg aatgaaatat tggttttggc
     3841 caacggagta aaacaagcaa gagattcttt atccaaacag attgctatca aaaaattata
     3901 tagaggctta tgcaaacatc aagctcaatt ggaggatgtt tggatttttt catttgatca
     3961 taaaaataca cgtgatttga ttaatcaaac attaaatacc atggacagtt taactgttct
     4021 gggtagagat gagggtgggc tggatattaa ttttattcag gagtgcaaag aagaagctca
     4081 atactatttg ttgggagaga aattcagctc tgttttagaa gactttgaaa aaaataacag
     4141 tcagatctta actgctcatt ctgaatggca aaaggttaaa aggcaacttc agcaaaagca
     4201 aaatgagagt cgcaaggttt atacattgaa tgaaatgtat tatgttcttt gtaaaaaaat
     4261 caatgaatta tccatttcat attccctatt gcgtgagcct ctagtgcaat tacgagggat
     4321 gattcgtact ctttggaata gttttcagga aaaacaccag gatatcatag atgaatcgaa
     4381 atattttgat tttaaagcgc gaaaaattaa ggaggagctg gagggaatag aaggatattc
     4441 tgtttttaac gtgaaagtaa aaaaaggata tacaggattt aatgagtatg ttgagttaat
     4501 tattgaagga gaagggacat ccgcattatt tgaagggttt atgcaatata aatccaggtt
     4561 aactgaatta aaagaggagt accgtcagtt agaattttct cttaagcaaa atagattaga
     4621 gttgctgaat ctgaatagaa tccatgccaa acacttacct tttttgaaag atcgcatcaa
     4681 gggcaatccc gaagtagagt tgtttccaga gcaatgccga agcaaggtga aggagatttt
     4741 aacactaaag gagtggatta atggccaaat acctgaaaac ctcagtcaat ttcctgaaaa
     4801 catgcaaaat cattttcttg acagaaatac gttaaaaaac atggatagaa atacgatgac
     4861 catggagcaa attgataaga taaaggatga gcagttaaaa gcggacctta cggaattata
     4921 taataaaatg cataaggtcg atgaagaaca gtcccaatca tggttttcta cagtgactcg
     4981 caggattgtt ggtttgattc ccagcatgtt cagacaggaa gagacagagg atgattggag
     5041 gtatcaattt aatgaattac aaggacgcac agatagcatt ttgcaatcgt tgttaactaa
     5101 aaaaataatg acaaaatatg atgcgttggt taatgaaatg gttttaatcc aaagcaatct
     5161 ggcacaacag atggactctg gtaacaaaaa aattcaattt ttaagcgata agattcttga
     5221 agaagaaagg aaaaccaatg tgattatcaa gcgttttgat aacctcaatg atttttatga
     5281 ttttgaggct aaactaaaac gtgttaaaac cgcgcctcca atagccccag taaatgaggt
     5341 tcagatagaa tctaaaaaca ggatactttc ttgtggtgag ggggcgtttt ccaatcctga
     5401 agaggagttt cagacggatt ctgtaatgca acctccaatg gtttttagta tttaacgtca
     5461 gtgatggata aggattataa aaccagttga tatccaaggt tcgttcgggc tgaggagatg
     5521 cataagcatc gtctcgaagc cttggcgcac aacattaaat cccgtacaag gcttcgagac
     5581 ggcataaatg cctcctcagc ccgaacgatt cgaggtgaca actgatctta tccataactc
     5641 acattaatga ataccaaacg agatatggtg aacctgggca aagaacttta tttgtgattc
     5701 tttaaataca agtcctgtat tattggcttc tctttaatgc cgttcgggtt atttttaatc
     5761 cgaacagcat tgccggaatg cagataacgc ggtgtcaatg gggtagaaaa tcttatttat
     5821 taattaactc aataataaaa ttaaccac
//
LOCUS       DACTJE010000183         5739 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.185, whole genome shotgun sequence.
ACCESSION   DACTJE010000183 DACTJE010000000
VERSION     DACTJE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.185"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            59..134
                     /locus_tag="I9760_002656"
     tRNA            59..134
                     /locus_tag="I9760_002656"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92..94,aa:Trp,seq:cca)
     gene            148..519
                     /gene="secE"
                     /locus_tag="I9760_002657"
     CDS             148..519
                     /gene="secE"
                     /locus_tag="I9760_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAT5044405.1"
                     /translation="MKSSNENQGKMKDIMSWLAIILATAGAFVCTYYYTFTGPIQSII
                     WLAWLILILFLGYLTTTGKRVFVFAQEAKVELLKVVWPTRQETIQTTTIVMVMVGLTG
                     FILWGVDSIMMWAIAKLTHLG"
     gene            522..1070
                     /gene="nusG"
                     /locus_tag="I9760_002658"
     CDS             522..1070
                     /gene="nusG"
                     /locus_tag="I9760_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAT5044406.1"
                     /translation="MVEENKAKQWYVVHAYSGYENFVMREITSRAKHHNLQDKIGEVV
                     VPSEEVVEMRSGQKRKSTRKFFPGYVLVHMVMDDQTWHMVRGIPRVLGFIGGTSQTPT
                     PISDKEAQAIMQRVEDGVTKPRPKILFEPGEVIRVKEGPFVDFNGVVEEVNYEKNRLR
                     VAVLIFGRSTPVELEFSQVEKT"
     gene            1181..1615
                     /gene="rplK"
                     /locus_tag="I9760_002659"
     CDS             1181..1615
                     /gene="rplK"
                     /locus_tag="I9760_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAT5044407.1"
                     /translation="MAKKVEAYIKLQIPAGKANPSPPVGPALGQRGVNIMEFCKAFNA
                     ATQQMEQGLPIPVVITVYSDRSFTFITKTPPASVLLKKAAGIQSGSGTPNTKKVAKLN
                     VSQLEEIAKVKKPDLTAADLAAAVRSIAGTARSMGIEVEGLE"
     gene            1625..2320
                     /gene="rplA"
                     /locus_tag="I9760_002660"
     CDS             1625..2320
                     /gene="rplA"
                     /locus_tag="I9760_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAT5044408.1"
                     /translation="MSKLTKKQKKIAEVIKPNQLYTVADAVAILKQFASKKFCESLDI
                     SINLGVDPRKSDQVVRSSTNLPKGTGTTVRVAVFAQGDNAAKATAAGADIVGFEDLAD
                     KIKAGEMNFDVVIATPDAMRIVGQLGQILGPRGLMPNPKVGTVTTNVEAAVNDAKSGQ
                     VRYRTDKNGIIHCTVGKADFAPEDVLENVVALINDLKKAKPASSKGQYLKKISLSTTM
                     GPGLPIDISSIPV"
     gene            2493..3026
                     /gene="rplJ"
                     /locus_tag="I9760_002661"
     CDS             2493..3026
                     /gene="rplJ"
                     /locus_tag="I9760_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAT5044409.1"
                     /translation="MTLNLAAKKAVVEEVTAVASKAISAVVADYRGLTVNQMTQLRSE
                     ARKSGVYLRVVRNTLTRRAFKNTEFECLNDLLVGPVFIALSLEAPSDAARLLKDYAKT
                     FEKLEIRALSVGGKVYNANQIDAVASLPTRDEAISKLMYVMKAPIEKFVRTLAEPHAK
                     LARTLAAVKDKKAGNPA"
     gene            3057..3437
                     /gene="rplL"
                     /locus_tag="I9760_002662"
     CDS             3057..3437
                     /gene="rplL"
                     /locus_tag="I9760_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAT5044410.1"
                     /translation="MAVSKNEILETISNMTVMEVVELIEAMEEKFNVSAAAAAVAVAA
                     PAAGAGAAAAEEQTEFTVVMTSFGSNKVNVIKAIRGITGLGLKEAKDLVEGAPSTVKE
                     GVSKDEAASIKKELEEAGATVEVK"
     gene            3529..>5739
                     /gene="rpoB"
                     /locus_tag="I9760_002663"
     CDS             3529..>5739
                     /gene="rpoB"
                     /locus_tag="I9760_002663"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAT5044411.1"
                     /translation="MAVAEAKPQYSHAEKKRFRKSFGKQTDIMPIPNLLEIQLKSYRD
                     FLQTDTKLSEQLNTGLHAAFSSVFPIESFSGNARLEYVGYKLGEPAFDVRECKLRGLT
                     YSAPLRVKIRLVVLDKDASDDPKPIKDIREQDVFMGEIPLMTDVGTFVVNGTERVVVS
                     QLHRSPGVIFEHDKGKTHSSGKLLYSARIIPYRGSWLDFEFDPKDCVYVRIDRRRKLP
                     VTILLRALGYEAEDILSEFFETTHCHLKNGEYHIDLIPQRLRGEIASFDIHVPETGEL
                     IVEQGRRITARHIKQMEKSQMQDLVVPRDYLIGKTLAKNIIDTSTGEFLAQANDEITE
                     ELLDAMANHGILQIDMIYTNDLDHGSYISDTLKIDPTGSQLEALVEIYRMMRPGEPPT
                     KEAAEALFKNLFFVEERYDLSAVGRMKFNRRVGIKSDEGPGTLTKEDILSVIKTLIDI
                     RNGIGMVDDIDHLGNRRVRSVGEMTENQFRVGLVRVERAVKERLSLVESENLMPQDLI
                     NAKPVSAAIKEFFGSSQLSQFMDQVNPLSGVTHKRRVSALGPGGLTRERAGFEVRDVH
                     TTHYGRVCPIETPEGPNIGLINSLSVYARTNEYGFIETPCRKVVNGRVTDEVEYLSAI
                     EEVDQYIAQSNVELDAQGNILADLVPCRHQNEFSLTTPDKINYMDVSPKQIVSVAASL
                     IPFLEHDDANRALMGSNMQRQAVPTLRSEKPLVGTGMERIVASDSGVSVVAKRGG"
BASE COUNT         1870 a         1018 c         1285 g         1566 t
ORIGIN      
        1 agaaaaaagt acaaaagtgg catgagcctt atatacatgg ctcatgtcaa taaaatttag
       61 gccagtagct caattggcag agtggcggtc tccaaaaccg caggttgggg gttcgattcc
      121 ctcctggcct gccaactaca agtaaatatg aaaagttcaa acgaaaatca aggtaagatg
      181 aaagatataa tgtcctggct agctatcata ttagctacgg caggagcttt tgtatgtact
      241 tactattata cgttcacagg accaattcaa tcgataatat ggcttgcttg gcttattttg
      301 atccttttct tgggttactt aacaacaaca ggtaaaagag tctttgtgtt tgcgcaagaa
      361 gcaaaagttg aattattaaa agtggtttgg cctacacggc aagaaacaat acaaacaact
      421 actatagtaa tggtgatggt tggattgaca ggatttatac tctggggagt agattcaatc
      481 atgatgtggg caattgctaa gttaacacat ttagggtgaa tatggttgag gaaaacaaag
      541 caaaacagtg gtacgttgta catgcctatt caggatatga aaattttgtc atgcgcgaaa
      601 taacatctcg tgcaaaacac cacaatttac aagataaaat aggcgaggta gttgtacctt
      661 cagaagaagt tgttgaaatg cgttcaggcc aaaagcgtaa aagtacccgc aaatttttcc
      721 caggctatgt actcgttcat atggtaatgg atgatcaaac atggcacatg gttagaggca
      781 tacccagagt tctaggcttt attggaggaa ccagccaaac cccgactccg atttctgata
      841 aagaagctca ggccattatg caacgagttg aagatggcgt aactaagcca aggccaaaga
      901 tactatttga gcctggagaa gtaatcagag tgaaagaagg tccatttgtt gactttaatg
      961 gagttgttga agaggtcaat tatgagaaaa acagactaag agtcgccgtt cttatttttg
     1021 gacgctcaac tccagtagaa cttgagttca gtcaagtaga aaaaacataa atgatttaga
     1081 taagaattta aatttaaagg ttttacaaaa ccggggagct agaacttaat tgaaaatgct
     1141 aattcaataa aagctagcgc atgacccata aggagtaata atggcaaaaa aagtagaagc
     1201 atatattaaa ttacagattc ctgcaggaaa agcaaatcct agccctccag ttggacctgc
     1261 tttaggtcaa cgtggggtaa atattatgga attctgtaaa gcattcaatg ctgcgacaca
     1321 gcaaatggaa caaggtctgc ctattccagt tgttatcact gtatatagtg atcgtagttt
     1381 tacctttata accaagaccc ctccagcttc agttcttctg aaaaaggctg ctggtattca
     1441 aagcggaagt ggcacaccaa atactaaaaa agtagccaaa ctgaatgtta gtcaacttga
     1501 agaaattgct aaggtcaaga aacctgatct gacagcggct gatctagcag cggcagtaag
     1561 aagtattgca ggtacagcac gcagcatggg tattgaagtt gaaggtttgg aataaggggt
     1621 tactatgtca aaattaacta agaaacaaaa gaaaatagcg gaagttatta agccaaatca
     1681 actctataca gttgctgatg ctgtagctat tctaaagcaa tttgcatcaa aaaaattttg
     1741 tgagagcctt gatatcagta ttaatttagg agttgatcct cgtaagtcgg atcaagtagt
     1801 acgatcttcc accaatctgc caaaaggcac aggaacaaca gtacgagttg ccgtttttgc
     1861 ccaaggtgat aatgctgcta aagcaactgc tgcaggtgcc gatatagtcg ggtttgaaga
     1921 tctggctgat aaaatcaaag caggcgaaat gaattttgat gttgttattg caactccaga
     1981 tgctatgaga atcgtaggtc agctcggaca gattttggga ccgaggggac ttatgcccaa
     2041 tcctaaggtt ggaacagtaa caactaacgt ggaagcagcg gttaatgatg ctaaatcagg
     2101 acaagtaaga tatagaactg ataaaaatgg aatcattcat tgtactgttg gaaaggcaga
     2161 ttttgctcct gaagatgttc ttgaaaacgt agttgcgtta atcaatgatc tgaagaaggc
     2221 aaagccagca tcctctaaag ggcaatatct gaagaaaata tctttatcta caacaatggg
     2281 acctgggtta cctattgata tttcatcaat acctgtgtaa catatcatcc catttttaag
     2341 aattcacggc atagattagg ttccatgccg tcgaagaccg caggtgccta ggcttaattt
     2401 cctgcgcaga cggtgaaccg gctccttaat aaagagacgg ccaccataac tgtcaagtcc
     2461 atgagatttg tacatattag gaggtcagta acgtgacatt aaatttagct gcaaaaaaag
     2521 ccgttgttga agaagtgact gcagtcgcgt caaaggctat ttcagcagtg gttgctgatt
     2581 atcgtgggtt aactgtaaat caaatgacgc aattgcgtag tgaagcacgg aaatccggtg
     2641 tttatcttcg tgtggtgcga aatacactaa caagaagagc gtttaaaaac actgaatttg
     2701 aatgcttgaa tgacttactt gtaggtccag tatttattgc attgtcactg gaagcaccaa
     2761 gtgatgctgc aaggctactg aaagattacg caaaaacatt tgaaaaactt gagattagag
     2821 cattatcagt tggcgggaag gtatacaacg caaatcaaat tgatgccgtt gcgagcttgc
     2881 caacacgcga tgaggcaata tccaagttaa tgtatgtgat gaaagcgcca attgagaaat
     2941 ttgttcgcac acttgcagag ccacatgcca agttggctag aactcttgct gctgtaaagg
     3001 acaagaaagc aggcaatccc gcttaatcat tatttaattt taattaggag ctagtaatgg
     3061 ctgtatcaaa aaacgaaatt ttagaaacaa tttctaacat gacagtgatg gaagttgttg
     3121 aattaatcga ggcaatggaa gaaaaattta atgtttctgc tgccgctgct gccgtagctg
     3181 ttgcagcacc cgctgcaggt gctggtgccg ctgctgctga agaacaaact gagtttactg
     3241 ttgtaatgac cagctttggt tctaacaagg taaacgtaat caaggcaatt cgtggtataa
     3301 ctggtctggg cttgaaagaa gctaaagact tagtagaagg tgcaccatca actgttaaag
     3361 aaggtgtatc aaaagatgaa gctgccagta tcaagaaaga gcttgaagaa gctggtgcta
     3421 cagtagaagt taaataatta aaatatagca gtatttaaga acccagccat gttcacatgg
     3481 ctgggtttac tcgtttgaaa atcatcaata atttacagag gaaccaccat ggcagttgca
     3541 gaagctaaac ctcaatattc gcatgctgag aaaaaacgat ttcgcaaaag ctttggtaag
     3601 cagacagata taatgccaat accgaatctg cttgaaattc aacttaaatc ctacagggat
     3661 tttcttcaaa ctgatactaa attaagcgaa caactgaata cgggattgca tgctgcattt
     3721 tcttctgtat ttcctatcga aagcttttct ggcaatgcaa gacttgaata cgtcggatac
     3781 aagcttggag agcctgcatt tgatgttcgc gaatgcaaac tacgagggct aacttactca
     3841 gctcctctaa gagtcaagat cagactcgtt gtgcttgata aagatgcaag cgatgatcct
     3901 aagcctataa aggatattag agagcaagat gtttttatgg gggaaattcc cctaatgacc
     3961 gacgttggta cgtttgtggt caatggaact gaacgtgttg ttgtttcaca attacatcgt
     4021 tcgcctggag ttatatttga acatgacaag ggaaaaactc actcttcagg taaattgctc
     4081 tattccgcac gaataatacc ttatcgtggt tcatggttag attttgaatt cgatcctaaa
     4141 gattgcgttt atgtacgtat tgacagacgt cgaaaactgc cagtgaccat tttgctcaga
     4201 gcattgggct acgaagcaga agatatatta agcgaatttt ttgaaacaac tcattgtcat
     4261 ctaaaaaatg gcgaatacca tatcgattta attccacagc gtttgcgtgg tgaaatcgcc
     4321 tcattcgaca ttcatgttcc tgagacagga gagcttatcg tagagcaagg tcgtcgaatt
     4381 actgcaagac atataaagca aatggaaaaa agccaaatgc aggatcttgt ggtgccaaga
     4441 gactatttaa ttggaaaaac attagcaaaa aatattattg atacctcaac cggtgaattt
     4501 ctggctcaag cgaatgatga gataacggaa gagcttttag atgctatggc taaccatggt
     4561 attttacaaa ttgatatgat ctatactaat gatttggatc atggttctta catatcggat
     4621 acattaaaaa ttgatcccac agggtcacaa ctagaagcgc tcgttgaaat ttaccgcatg
     4681 atgcgccctg gagagcctcc tacaaaagaa gctgctgagg cactattcaa aaatctattc
     4741 tttgttgaag aaagatatga tctatctgct gttggcagaa tgaaatttaa tcgtcgcgta
     4801 ggaattaaga gtgacgaagg cccaggcact ttaacgaaag aagatatttt atctgtcatt
     4861 aaaacactga tagatattcg aaacggtata gggatggttg atgatatcga tcatctagga
     4921 aatcgtaggg taagaagtgt tggcgaaatg accgaaaatc agtttagagt aggtcttgtt
     4981 cgagttgaac gtgctgttaa agagcgctta agtttggttg aatctgaaaa tctgatgcca
     5041 caggacttaa ttaacgcaaa accagtatcc gccgcaatta aagagttttt tggatccagt
     5101 cagttatcac agtttatgga tcaagtgaat ccattatctg gtgttacgca taaaagacgt
     5161 gtatcagctc ttgggccagg tggtttaaca cgtgagcggg ctggatttga agtccgtgac
     5221 gtacatacaa cacactatgg tcgtgtgtgt ccaattgaaa cgcctgaagg accaaatatt
     5281 ggtctaatca attcactgtc agtttatgca agaactaacg aatatggatt tattgaaact
     5341 ccttgtcgaa aggtcgttaa tgggcgtgtg acagatgaag ttgaatacct atcggctatt
     5401 gaggaagttg atcaatacat tgcacaatcc aatgttgagt tagacgcaca aggaaatatt
     5461 cttgctgact tagttccttg cagacatcaa aatgaatttt cactgactac tccggataaa
     5521 attaactaca tggatgtctc gcccaagcaa attgtgtcag ttgctgcttc attgattccg
     5581 tttttagagc acgatgacgc aaacagagca ttaatgggtt caaacatgca acgacaggct
     5641 gtaccaacct tgcgttcaga aaagcccttg gttgggaccg gtatggaaag aatcgttgca
     5701 tctgattcag gagtctcagt tgttgcgaag agaggtgga
//
LOCUS       DACTJE010000184         5671 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.186, whole genome shotgun sequence.
ACCESSION   DACTJE010000184 DACTJE010000000
VERSION     DACTJE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.186"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1070)
                     /locus_tag="I9760_002664"
     CDS             complement(<1..1070)
                     /locus_tag="I9760_002664"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT5044412.1"
                     /translation="MSELILILAAIGVAGTLLTRHASISVWAISYTIFLGLVLQFSSP
                     GLLSQIVLWAIEAVLVLGSIKPLRREVLSKHLFQIIKKTMPSMSITEREALEAGTVSW
                     EGDLFSGNPNFSLLRNAPTVALTEEEQAFIDGPVSTLCEMLDDWDITHNRTDLPPEVW
                     QFIKENRFLGMIIPKQYGGLGFSATAQMSILVKIYGRSITAATTISVPNSLGPGELLL
                     KYGTEEQKNYYLPRLADGREIPCFALTGPNAGSDAASIPDKGVVCRQEFNGELILGVR
                     LNWDKRYITLCPVATVIGLAFRMFDPDNLLGKGEDIGITCALIPATTPGITKGRRHFP
                     LNTGFMNGPTQGKDVFIPLDYL"
     gene            complement(1163..1948)
                     /locus_tag="I9760_002665"
     CDS             complement(1163..1948)
                     /locus_tag="I9760_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5044413.1"
                     /translation="MRELIHFAHGNGFPSLCYKQLLEQLETRFDCCFIDKIGHDPDYP
                     VGENWHNLVAEIIASIKRQAEQPVIAVGHSLGGVLSLLAAIEQPKLFKAVIMLDSPLI
                     GAFKSSMVRLAKALGIIDRVTPAFRTKKRREHWQNYEQLISYLKTRDLFKTFTEECLN
                     DYIKYGLQYKEDGYYLRFDRHVEYQIYRTIPHVIPRYKGKLIVPAALIYGDKSTVVDR
                     MDIRYMKNHFNVKCFKTMGTHLFPMEHPKAVGKQVIEVIDAII"
     gene            complement(1945..2862)
                     /locus_tag="I9760_002666"
     CDS             complement(1945..2862)
                     /locus_tag="I9760_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAT5044414.1"
                     /translation="MYLMREQENVWESGVAKEKNKLKPIDAPIYSYWQALYMSFYSKR
                     LFVDVGKHWRGIGLIYLLLVIAICSIPFSIRIASDFNKTFNQQIIEPLLQLPTVYVQK
                     GNVIFDKPMPYLIRNDKNQVVIIIDTTGKVVDFTEEYPYLNILIKKNSINFLLPTPEL
                     LGSSPVNEQKGVPIVQPLDKDMNVVFDGKKIIQDNAISGLKYASQLMIYPIVVAILYS
                     IMVTVLLVIAFLGQVFSKIFFSFTISFKKSSRLLMVASTPMLVVLVTMLSLHFIFPGF
                     GVILLILLAAYYSYGIYGLRDESRKMVLK"
     gene            complement(2813..3904)
                     /gene="ugpC"
                     /locus_tag="I9760_002667"
     CDS             complement(2813..3904)
                     /gene="ugpC"
                     /locus_tag="I9760_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAT5044415.1"
                     /translation="MATLNLIEVSKQVGETTILNRINIDIKKGEFMVVVGPSGCGKST
                     LLRLIAGLDVLSSGKILINNQCVNDTEAAKRDMAMVFQNYALYPHMTVFDNMAYGLKL
                     RRFSKADINQRVTEVANLLHLTPYLDRKPQALSGGQKQRVAMGRAIVRSPAVYLFDEP
                     LSNLDAKLRTEMRHEIKRLHQQFNTTSLYVTHDQTEAMTMADRILVLNQGEVEQIGTP
                     QELYQSPATQFVAGFTGHYPINFISGKYDKKTDSIYTEIGIVYSAPNWLHSVDDNADL
                     IVAIRPEHIQLCSSQSQDAVELHVEFVDDMGADKLIQAKCLKNNKQINLRISAEQMVS
                     NRQLHVELPKLKLHVFDERTGKRVGEWRG"
     gene            complement(3904..4731)
                     /gene="ugpE"
                     /locus_tag="I9760_002668"
     CDS             complement(3904..4731)
                     /gene="ugpE"
                     /locus_tag="I9760_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAT5044416.1"
                     /translation="MRQFKRLGSHGLILLFIGLMMAPLYLAVVAASHEGTVMMHSQLP
                     LIPGTSFFKNIKSVLTEGLAVTGGEPITSMLVNSLFMAFIIAVGKIILALGSAFALVY
                     FDFSFKKSCFALIFATMMLPVEVRIVPTFQVVASFGLLNSFSGLTLPLLASATGTFLF
                     RQFFKTISKELVDAAKLDGAGPLRFFIDIVLPLSRTQISALFVILFVYGWNQYLWPLV
                     ITTETKMTTIVMGIRYLAGVADQVPEWHYIMAVALIALVPPCMVVMVMQRWFEKGLK"
     gene            complement(4728..5606)
                     /locus_tag="I9760_002669"
     CDS             complement(4728..5606)
                     /locus_tag="I9760_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAT5044417.1"
                     /translation="MSKFNHQRLFAWLFIVPQLIVTIIFFIWPACSALLQSFFYTDAF
                     GLHKNFAGLTNFFDLFLDPGYAKAIWVTFIIAISVTLITMSLGLLMATLINYRGKSQG
                     VYKTLLIWPYAIAPAVAAILWRFLCHPTLGWLTQVLEYLGIHFNYVNDANQALLVVIL
                     TASWQQFSYNFLFYFAALKAVPQTLIDAAIMDGANTWQRFWQIIFPILSPTTFFLLIM
                     NLIYGFFDTFGIIQVMTHGGPGNSTTNLIYKVYEDGFEGMDLGSSSAQSVLLMLIVIA
                     VSLLQFKYLEKKVHYE"
BASE COUNT         1906 a         1191 c          916 g         1658 t
ORIGIN      
        1 atcaaataat caagcggaat aaaaacatct ttgccttgag ttggaccatt cataaaccct
       61 gtatttaaag ggaagtgcct gcgccctttt gtgatccccg gagtagttgc aggaataagc
      121 gcacaagtaa taccaatgtc ttcacctttg cctagcaagt tgtcgggatc aaacattcta
      181 aaagccaaac caataactgt cgccaccggg cacaaggtaa tatatcgttt gtcccaattc
      241 aaacgaactc ccaatatgag ctcaccattg aactcctgcc ggcaaacaac acctttatca
      301 ggtatagagg ccgcatccga accagcatta ggtcctgtca aggcaaagca tggaatctcc
      361 cttccatcgg ccaatcttgg taaataataa tttttttgtt cttctgtccc atactttaaa
      421 agtaattcac caggccccag tgaattaggt actgaaatag ttgtagcagc agtaatagaa
      481 cggccataga tttttaccaa aattgacatc tgagcagtag cggaaaaacc aagtccacca
      541 tactgctttg gaattatcat tcccagaaaa cgattctctt ttataaattg ccaaacttcg
      601 gggggaagat ccgttctatt atgagtgata tcccaatcat ctaacatttc acagagggta
      661 ctgacagggc cgtctataaa agcctgctct tcttcagtta atgcaacagt tggagcattc
      721 ctcaaaagag agaaattagg attaccacta aataaatctc cttcccaact cactgtacct
      781 gcttccaatg cctctctctc agtaatagac atggaaggca tcgttttttt aattatttga
      841 aacaaatgtt tcgatagtac ttctcttctt aaaggcttaa tggagcctaa aaccagtaca
      901 gcttcgattg cccataagac gatttgcgac aacaaaccag gagaactgaa ttgcaaaacc
      961 aagccaagaa aaatggtata actaatagcc catacagata tcgatgcatg tcgggttaat
     1021 aacgttccag ccacaccaat tgctgctagt attaatataa gttctgacac aacattctcc
     1081 ttattatagg ctaatccaaa tgaaagaatt taatagactt ctttctcgat aacaaaatct
     1141 gcaaaccagt taagacgtct aatcaaatta tagcatcaat aacttctata acttgctttc
     1201 caacagcttt agggtgctcc attggaaata aatgtgtacc catagtttta aaacatttta
     1261 cattaaaatg atttttcata tatcgaatgt ccattctatc aactacagta ctcttgtcac
     1321 catagattaa tgcggcggga actataagtt tacctttata acgaggaata acatgcggaa
     1381 ttgttctata tatttgatat tcaacatgcc tgtcaaaacg cagatagtat ccgtcctctt
     1441 tatattgcaa accatattta atatagtcat tcaagcattc ttctgtaaat gttttaaata
     1501 aatccctggt ttttaaatag gaaattaatt gttcatagtt ttgccaatgc tcacgccttt
     1561 tctttgttct aaaagcaggg gttactctgt ctatgatacc taaagcctta gccaatctca
     1621 ccatgcttga tttaaaagca ccaatgagag gcgagtctag cataatgacc gctttaaata
     1681 atttcggttg ctctatcgca gcgagcaaac tcaaaacccc tcccaaagaa tggccaactg
     1741 caatcactgg ctgctctgct tgtcgtttaa tgcttgcaat gatctcagca accaggttat
     1801 gccagttttc tccgacagga taatcaggat catggccaat tttatctata aaacaacagt
     1861 caaaacgagt ttccagctgc tctaataact gtttgtaaca taatgatgga aatccattgc
     1921 catgagcaaa atggattaat tctctcattt gagcaccatt ttcctgcttt catctcggag
     1981 accatagata ccgtaactgt aataggctgc caataatatc aacaaaatca ctccaaatcc
     2041 tggaaaaata aaatgaagtg ataacatggt taccaaaact actaacattg gcgttgatgc
     2101 gaccattaac agtcggctgg attttttaaa ggatatagtg aacgaaaaaa agattttgga
     2161 aaatacttgt cctaaaaagg caatgaccaa taaaacagtc accataatcg aatataaaat
     2221 agcaaccact attggatata tcatcaattg agaggcatat ttcaaaccac taatcgcatt
     2281 atcctgaata atttttttgc catcaaatac cacattcatg tctttatcta atggctgaac
     2341 aataggaact cctttctgtt catttactgg gctgctaccc aataattctg gtgttggcaa
     2401 taagaaatta atactatttt tcttaatcag aatattaagg taaggatact cctctgtgaa
     2461 atctaccact ttacctgttg tgtctataat tattaccact tgatttttat catttctgat
     2521 taaatagggc attggtttat caaaaataac attacctttt tgtacataaa cagttggcag
     2581 ctgcagcaaa ggttcaatga tttgctgatt aaacgtttta ttaaaatcag aagcaattct
     2641 tatagaaaac ggtattgaac aaatagcaat aaccaataat agataaatca aaccaattcc
     2701 acgccaatgt tttcctacat caacaaaaag cctctttgaa taaaaagaca tatacagcgc
     2761 ttgccaataa ctatagatag gagcatcaat cggttttaat ttattttttt ctttagccac
     2821 gccactctcc cacacgtttt cctgttctct catcaaatac atgcaatttc aatttgggta
     2881 gctctacatg taattgtctg tttgagacca tctgctctgc tgaaattctc aaattaatct
     2941 gtttattatt ttttagacat ttcgcctgaa tcaatttatc agcccccata tcatcaacaa
     3001 attccacatg aagttcaact gcatcttgac tctgactgga acatagttga atgtgttcgg
     3061 ggcgaattgc cacgataaga tctgcattgt catcgacaga atgaagccaa ttgggtgctg
     3121 aataaactat acctatttcg gtataaatac tatccgtttt tttatcgtat ttgccagaaa
     3181 taaaattaat aggatagtgc ccggtaaaac cagctacaaa ctgcgtagcc ggactttgat
     3241 acagttcttg tggcgttcca atttgctcaa cttccccctg attaagcacc agaattctat
     3301 cagccatagt catcgcttcg gtttgatcat gagtaacgta taaactggtc gtattaaatt
     3361 gttgatgcaa tcgtttaatt tcatgtcgca tttcagttct taattttgca tccagatttg
     3421 ataatggttc atcaaataaa taaactgctg gagaccgcac aatagcccta cccattgcta
     3481 ctctctgctt ttgtccacca gacagtgctt gcggctttct gtccagatat ggagtcaaat
     3541 gcaataaatt agctacttcg gtcactcttt gattaatgtc tgctttactg aagcgcctca
     3601 actttaaacc ataagccata ttatcaaaga ccgtcatatg aggataaagg gcataatttt
     3661 gaaacaccat cgccatatcc ctttttgcgg cttcggtatc attaacgcat tgattattaa
     3721 taagtatttt acctgagctt aaaacatcaa gtcctgctat caatcgtaac aaagtagatt
     3781 tgccacaacc tgatgggcca acaacaacca tgaattcgcc tttctttata tcaatgttaa
     3841 ttcgattcaa aatggttgtt tctccgacct gttttgacac ctcgatcaaa ttcagtgttg
     3901 ccattatttc aatccctttt cgaaccaacg ttgcatcacc ataaccacca tacaaggcgg
     3961 cacaagagct atcaatgcga cggccataat atagtgccac tccggtactt gatccgctac
     4021 gcctgccaaa taccgaattc ccatcacaat agttgtcatt ttagtttctg tcgtgatgac
     4081 caaaggccat aagtattgat tccacccata gacaaacaag attacaaaca aggcggaaat
     4141 ttgagttctt gacaagggta acacgatatc aataaaaaat cttaatggac ctgcgccatc
     4201 taatttagca gcatcaacca gttctttaga tatggtttta aaaaactgac gaaataaaaa
     4261 tgttccagta gctgatgcca ataatggcaa ggttaaacca gaaaatgagt tcaataggcc
     4321 aaatgaagca accacttgaa aagtagggac tattcttact tccactggta acatcatcgt
     4381 tgcaaatatc agggcaaaac aagacttttt aaaagaaaaa tcaaaatata ccaatgcaaa
     4441 agcagatccc agagccaaaa tgatcttacc cactgcaatg ataaatgcca tgaataagct
     4501 atttacaagc atggaagtga taggctcacc tccagtgaca gctaaacctt cagtcagcac
     4561 acttttaata tttttgaaaa atgaggtccc tggaatcaat ggcaactgag aatgcatcat
     4621 gaccgttcct tcatgacttg cagcaactac agccaaatac aaaggagcca tcattaatcc
     4681 tataaataac aatattaacc catgcgaccc caaacgttta aattgtctca ttcgtaatga
     4741 acctttttct caaggtattt aaattgtaac aaggaaactg caattacaat aagcatcagt
     4801 aatacagatt gggctgatga acttcccaaa tccattccct caaaaccgtc ttcatacacc
     4861 ttatagatta aattagttgt gctattacca ggacctccgt gtgtcatgac ttgaataatt
     4921 ccaaatgtat caaaaaaacc ataaatcagg ttcattatta acagaaaaaa ggtagtgggc
     4981 gacaggattg ggaaaattat ttgccagaat cgttgccatg tattggcccc atccatgatt
     5041 gctgcatcaa ttaatgtctg aggaactgct ttaagcgcag caaaataaaa taaaaaattg
     5101 taactaaact gctgccaact ggcggttaaa atgacaacaa gcaaagcctg attcgcgtcg
     5161 ttgacataat taaaatgaat tcccaaatac tcaagcacct gagttaacca tcctaaagtt
     5221 ggatgacata aaaaccgcca aagtattgca gcaacagcag gggctattgc ataaggccat
     5281 atcaacaaag tcttataaac tccttggctt tttcctctgt aatttattaa agtagccatt
     5341 aaaagaccta aactcatagt gataagcgtc acactaatcg caattataaa agtaacccat
     5401 atggcttttg cataaccggg atcaaggaat aagtcaaaaa aattggtcaa tccggcaaaa
     5461 ttcttatgca aaccaaatgc gtcagtataa aaaaaggatt gcaataatgc gctgcaagca
     5521 ggccaaataa aaaaaataat agtgacaatt agctgaggga ctataaatag ccaggcaaac
     5581 aaacgctgat gattaaattt agacattaac taaccatgta gttgttaaaa tgataattat
     5641 catcgcaata gtttaaaata aaattatatc g
//
LOCUS       DACTJE010000185         5573 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.187, whole genome shotgun sequence.
ACCESSION   DACTJE010000185 DACTJE010000000
VERSION     DACTJE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.187"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1448)
                     /locus_tag="I9760_002670"
     CDS             complement(<1..1448)
                     /locus_tag="I9760_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAT5044418.1"
                     /translation="MSTVEQRMPKGIMSLYLIQMFSTFSFAVLYSSLSLYITNQLGLS
                     NHISNSIVGLFLAFNFVLHLFGGLIGGNWLSNRFLFLFTTILQTFGILCLVFPETPSL
                     YLGLSLFLIGCGLNTTCYNTILTQRFSSSDPRRDKAFFMSYSTMNIGFWAGFICSGFY
                     DASNHYEEIFYASIATNIITTLLVLYCWKNITDINTALALNPAQKQRIKGIGGILFIL
                     LLIPVLNICFKLPDFSNGLVITISVLMFFVILFIRNNQKILSDRQKITAFLILTITST
                     VFWMIYYTGPMGITLFIKNNVDKQLLGFEIPTQWILNINSVVIIIGSPLIALLISKLQ
                     NKGYTFSVSTQFIWAFLFLTISFFSLSCGIHFANEAGYTAFSWIILHLVTQAVAELFI
                     GPVGYAMIGRIAPNHLQGLLMGSWMLVSGVSASLSHYFSNSMIQSESSDPLLSNSDYY
                     HVFNELAVWGLAGALFLYLIAGKLRLFMNERS"
     gene            1566..2867
                     /locus_tag="I9760_002671"
     CDS             1566..2867
                     /locus_tag="I9760_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044419.1"
                     /translation="MNKIERTSWDEIKQVVFAVNPALYEVIEKISPNKNIPFFLAHYS
                     FGSHFGIKNHAFLPTPEGTLEKIDSKHTSNELFEHLGYGKNSLPLGMILNKYCEWHHF
                     GSDDHIFPDCVQGPGAIFNMQVVFDEDQTVDNNVLSVSSGALSSFLLPNIGCTRRHSR
                     IQKYFNVDVPAPKSPYEHHLIFKEILQDKTLRSDWTSQILYFSQEFIDEVKTNDNWLK
                     LKLYFSESLRKKLTQNAYDASCNELFLRAKKINRFRPTPFVIDTAKYIYNICMGSGIG
                     VKPAIDDQYFPVTTIQKIYDECYQLEHTPTVMVPAPLSEKDDSVYYPLQCPFSKINTF
                     KTNQSNSTINELETLRNVLLAYQEEFIDVNGDAYGSPLYHMSKQIKFNFYHYKAPGNG
                     VIINSASLLDTDKRFSFMFDKSNRSFATDAKLFRGCVSLSR"
     gene            complement(2835..3635)
                     /locus_tag="I9760_002672"
     CDS             complement(2835..3635)
                     /locus_tag="I9760_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HAT5044420.1"
                     /translation="MHKLIVVGSGIKSVAHLTEETKKIIQNSDKVLYLVNEEFLKQWI
                     IRESKSAESLEPIYFNSTKRIDAYANITSKIVSSYYQYTSLCVIFYGHPTIFAESALK
                     AVKIIQAEKGNAIILPAISSMDCLFSDLQVDPGEQGCFSIDATELLIYERKLDIQSHV
                     IIWQIANLGMFNLEKTTKLNVLKDYLCSDYSQEQLVCLYEAALYPTQKPRIEWLKLSD
                     LGNVKVSPISSLYIPPSSTKKLSAKYIALLEIDLDNFKLSTETHTAPK"
     gene            complement(3640..3777)
                     /locus_tag="I9760_002673"
     CDS             complement(3640..3777)
                     /locus_tag="I9760_002673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044421.1"
                     /translation="MNLLDDLEALIGIEFLIENESTIIEITTEARQTEQPHANKIALI
                     Q"
     gene            4143..4337
                     /locus_tag="I9760_002674"
     CDS             4143..4337
                     /locus_tag="I9760_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT5044422.1"
                     /translation="MDKRVFKEVEAANYICMSRSFLSQDRVNGTLKNRTPGPKFIKIG
                     RAIRYLKEDLDIWLDLHRKP"
     gene            4420..5067
                     /locus_tag="I9760_002675"
     CDS             4420..5067
                     /locus_tag="I9760_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAT5044423.1"
                     /translation="MWLQRKSLKHKSMCDPVHGIMCVENELYITIKRVIDSHYFQRLR
                     QIKQLSFAEFAYPGAVHNRFSHSIGACYLSTVVFSTIYNDSTNNEDQKICLALTALIH
                     DIGHGPFFHSFEKIFNYALNFVDNKIGKISHEDWTQVFLKKLIEENYIERNYGRVVED
                     IYTKETNKLHGIISSQLDVDRFDYLLRDSHFYGVSYGNFDLLWLINRLCEQDGVT"
     gene            complement(5314..>5573)
                     /locus_tag="I9760_002676"
     CDS             complement(5314..>5573)
                     /locus_tag="I9760_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HAT5044424.1"
                     /translation="NCIRIDMKDSHIIKTSRDRVIFLNNDESASFIEFIRSNNLKNVE
                     DGKRVYFSLRNIKKVILHSGLDQETKLSIEKSINNKTLNAK"
BASE COUNT         1905 a          859 c          970 g         1839 t
ORIGIN      
        1 tttgaccttt cattcatgaa taaacgtaat tttcctgcaa ttaaatacaa aaatagtgcg
       61 cctgctaatc cccacactgc gagctcatta aatacgtgat aataatctga attacttaac
      121 aaaggatctg atgattcaga ttgaatcatt gaattcgaaa aataatgaga taaggatgca
      181 gatacacctg aaacaagcat ccagcttccc attagaagcc cttgtaaatg gtttggagct
      241 atacggccaa tcattgcata gcccacaggt cctataaata attccgctac tgcttgggtc
      301 actaaatgca aaataatcca gctaaaagcc gtatagcctg cttcattagc aaaatgaatt
      361 ccacatgata aagaaaagaa tgatatcgtt aagaaaagaa atgcccaaat gaattgggtt
      421 gaaacagaaa acgtgtaacc tttattctgt agtttgctaa ttagtaacgc gatcaacggg
      481 ctgccaataa tgataactac ggagttaata tttagaatcc attgtgtggg aatttcaaaa
      541 cctaaaagtt gtttatcaac attattttta ataaacaaag taatacccat tggacccgta
      601 taataaatca tccaaaacac agttgatgta atggttaaaa ttaaaaaggc agtaattttt
      661 tgtctgtctg agagtatttt ttgattgttt ctgataaata aaataacgaa gaacattagc
      721 acactaatcg ttattactaa gccattacta aaatcaggta acttaaagca aatattcaag
      781 acaggtatga gaagtaatat aaataaaata cctccaatac cttttattct ttgtttttgc
      841 gcagggttta aggctagggc ggtatttatg tcagttatgt ttttccagca atagagaact
      901 aataatgtgg taattatgtt agttgctata ctagcgtaga aaatttcttc gtagtgattg
      961 gaggcatcat aaaagccact acaaataaaa ccagcccaaa agcctatatt catagtggaa
     1021 tagctcataa aaaaagcttt gtctcgtctt ggatcggaag atgaaaaacg ttgggtgaga
     1081 atcgtgttat aacacgttgt atttaatccg cagccgatta aaaacaaact caatcctaga
     1141 taaagtgagg gggtttctgg aaatactagg catagaatgc caaaggtttg caaaatagta
     1201 gtgaataaga aaagaaatcg attacttaac caattgccgc ctattagccc gccaaataga
     1261 tgcaaaacga aattaaatgc taaaaataac ccaacaatac tattggatat gtggtttgat
     1321 aaacctagtt ggttagtgat gtacaaggac aatgaggaat aaagcactgc aaatgagaat
     1381 gtggaaaaca tttgtattaa ataaagagac attatacctt taggcattct ttgctctaca
     1441 gtactcattt gacctcctat ccgtgagccg tgactctaac attagaatta gttaagatta
     1501 agtgtttttt taatttttga tgtaatactg ttcacgagtg gaacaagttg ttgggtgttt
     1561 atggaatgaa caagatagaa agaacttcat gggacgagat caagcaggtt gtttttgcag
     1621 taaaccctgc gctgtatgaa gttattgaaa aaatttcacc aaataaaaat atcccttttt
     1681 ttctagctca ctattcgttt ggttctcatt ttggtattaa aaatcatgcc ttcttgccta
     1741 ctccagaggg aactttagaa aaaattgata gtaagcatac ctccaatgag ctattcgagc
     1801 acttaggata tgggaaaaat agcctgccgt taggtatgat tttaaacaaa tattgtgagt
     1861 ggcatcactt cggttctgat gatcatattt ttcctgactg tgttcaaggg cctggggcca
     1921 tattcaatat gcaggtggta tttgatgaag atcaaacagt agataataat gttttgtcgg
     1981 tctcatcagg agccttatca tcattcttat tacctaatat aggttgtaca agaaggcact
     2041 ctcgaattca aaaatatttt aatgtagatg tacctgcacc taaatcgcct tacgaacatc
     2101 atctgatttt taaagaaatt ttacaggata aaacactccg ttctgattgg actagtcaaa
     2161 ttttatattt ctctcaagag tttattgatg aagttaaaac gaatgataat tggttgaaat
     2221 taaagcttta tttttctgaa tccttaagaa aaaaattaac tcaaaatgct tatgatgcat
     2281 cttgtaatga gctatttttg agagcaaaaa aaattaatag attcaggcct acaccatttg
     2341 ttatagatac tgctaaatat atatataaca tttgtatggg ttctggtatt ggagtaaaac
     2401 cagcaataga tgatcaatat tttcctgtca caactataca aaaaatatat gatgaatgtt
     2461 accagctaga gcatacgcca acggtaatgg tgcctgctcc tttgtctgaa aaagatgata
     2521 gtgtctatta tcccttgcaa tgcccatttt ctaaaattaa tacatttaaa acaaatcaaa
     2581 gtaatagcac tataaatgag ttagaaactt taagaaatgt tctgctggcc tatcaggaag
     2641 agtttatcga tgtgaatggg gatgcctacg gcagccctct gtatcatatg agcaaacaaa
     2701 taaagtttaa cttctaccat tataaagccc cgggtaatgg ggttattatt aactctgcta
     2761 gcttattgga tactgataaa cgattttctt ttatgtttga taaatccaac aggagttttg
     2821 ctaccgatgc caaattattt aggggctgtg tgagtctcag tagataattt aaaattgtct
     2881 aaatcaattt ctaatagagc aatatatttc gcgctcaact ttttggtaga ggagggggga
     2941 atataaagcg aggaaatagg acttaccttc acatttccta aatcagatag tttgagccat
     3001 tcaattctag gtttttgagt tgggtatagg gctgcctcat ataagcaaac aagctgttct
     3061 tgtgagtaat cactacaaag gtaatctttt aatacattta gtttagttgt cttttctaga
     3121 ttaaacatac ctagatttgc aatttgccat atgattacat gggattgaat atctaattta
     3181 cgttcataaa ttagtagctc agtagcatca atactaaaac aaccttgctc cccgggatct
     3241 acttgtaaat cagaaaataa gcaatccatc gaggatattg cagggagtat gatggcattg
     3301 cccttttctg cttggattat tttaacagct tttaaagctg attcagcgaa gattgtggga
     3361 tgcccataaa aaatgacgca tagactagta tattgataat aactacttac aatttttgaa
     3421 gttatattag cgtaggcatc tattcgcttg gttgaattaa aataaatagg ttccaaggac
     3481 tctgcacttt ttgactctct tataatccac tgttttaaaa attcttcatt aaccaaataa
     3541 agcactttat ctgagttttg aatgattttt ttagtttctt cagtcaggtg tgcaacagat
     3601 tttataccgg aaccaacaac aattagttta tgcatttaat tattgtatta atgcaatttt
     3661 atttgcatgg ggttgttcgg tttgtctagc ttctgtagta atttctatta ttgtagattc
     3721 attttcaatt aaaaactcaa taccgatcaa tgcttccaag tcatcaagta agttcatcct
     3781 ttcaccctta atttaattga tttttgcgat cattgtataa acaaatggaa caaaggtata
     3841 gcgagattta aagatcttta aaatcatcaa aaaaagaatt gcgttaaaaa actcgttatg
     3901 tttaaataaa tacacgaaac gagtaattgg caaatctaaa gatagtaatg aaagaattca
     3961 tgaatggaca actgagtaac tcccaagtct ctcactttaa tccaatgaat attaatcaag
     4021 cctcaagatt tctgggtact cgtaaaaaat aatcagtcta tttgtagcag aagacacatt
     4081 atttgctgtt aaaagtggca ttggtgtgtt tatagataac tcgttatata aggatgtata
     4141 tgatggataa aagagttttt aaagaagttg aagccgcaaa ttatatttgc atgagtcgat
     4201 cctttctttc tcaagacaga gtgaatggca cattaaaaaa tagaactccg ggacctaaat
     4261 ttataaaaat agggcgagca attcgttatt taaaagaaga tttagatatc tggttagatc
     4321 ttcatagaaa accgtaaatg cggttttcta ttctatgaac aataataatt tttatttgat
     4381 gtaatctact tatatttact ttgtctataa tttaatcaca tgtggcttca aaggaagagt
     4441 ctgaagcata aaagtatgtg tgatccagta catggaatta tgtgcgttga aaatgagcta
     4501 tatattacga ttaagcgagt tatcgattcc cattactttc aaagacttcg tcaaattaag
     4561 caattatcat ttgcagagtt cgcctatccc ggcgcagttc ataatagatt tagtcattca
     4621 ataggggctt gctatctttc gacagtagtt ttttcgacga tttacaacga ttctacaaat
     4681 aatgaggatc aaaaaatatg tctagcgctt acagctttaa tacatgatat agggcatggc
     4741 ccattctttc attcctttga aaagatattc aattatgctt taaactttgt tgataacaaa
     4801 ataggcaaaa ttagtcatga ggattggact caagtttttt taaaaaaatt aatagaggaa
     4861 aattatattg aaagaaatta tggaagagta gtagaagata tatatacaaa agaaactaat
     4921 aaattacatg gaattatctc atcacaactc gatgtagatc ggtttgatta tttactaagg
     4981 gacagtcact tttatggtgt tagttatgga aattttgatt tactctggtt aattaatcga
     5041 ttatgtgaac aagatggagt gacgtaatag tatggatccc tcatcttgat tgcatgaatg
     5101 ttgacacctt cattctggct caaatagaag cctttcgata ggtggcgggg tccatactat
     5161 tacgtcatta atagcaatat taaatataga aattactaat aagtgctagg tcgattagtg
     5221 gaattttttg gaacaaagaa aaatggtaat taaacaacag gttgtttaat tgaagaattt
     5281 tttaccatgg agttgtatat aaattgcttg atactacttc gcatttaagg ttttattatt
     5341 aattgatttt tctatactca atttagtctc ttgatctagt cctgagtgaa ggataacctt
     5401 ttttatattt cgaagggaaa aatacactct tttcccatcc tccacatttt tcaaattatt
     5461 acttctaata aattcaataa aactggcaga ttcatcgttg tttaagaata ttaccctatc
     5521 tcttgaagtt ttgataatat gactgtcttt catatcaatc ctaatgcagt ttc
//
LOCUS       DACTJE010000186         5554 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.188, whole genome shotgun sequence.
ACCESSION   DACTJE010000186 DACTJE010000000
VERSION     DACTJE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.188"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            126..1196
                     /locus_tag="I9760_002677"
     CDS             126..1196
                     /locus_tag="I9760_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5044425.1"
                     /translation="MKTGRFDQAQADAFAERLLSVLNDAALTLMISIGHRTGLFDVMD
                     KLDFASSEVIAEAAGCNERYVREWLNALVVGGIVDYDAHTKAYQLPAEHASFLTRKSS
                     PQNMAVVAQFIPVLAYVENGIVDAFKHGGGVPYEAYHRFHEVMAEESGQTILSSLTDK
                     ILPMVHGLVDKLNQGIQVLDIGCGYGRALILMAKTYPQSQFTGYDLCDETIAHAVHSA
                     KKNKIQNIHFEKMDLSVWDEQEKYDLITSFDVIHDQAKPAQVLSNVFKALKPHGIFLM
                     QDIKTSSDVAGNVHHPLGALIYTISCMHCMTVSLSQNGLGLGAAWGEELAEKMLVEAG
                     FGKIEKHYLEHDIMNTYYVMRK"
     gene            complement(1324..1683)
                     /locus_tag="I9760_002678"
     CDS             complement(1324..1683)
                     /locus_tag="I9760_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044426.1"
                     /translation="MSNKAKNTHLKNGDSQTKQAYTNQASPLFFSQKQITPSSGTLET
                     KKPQEGKQPTQEISLETTTDILLAAILQELKTQNMIELMKLKAQQEHEQEELETIEAM
                     KEKDDARFNEIRQTMYA"
     gene            2082..2546
                     /locus_tag="I9760_002679"
     CDS             2082..2546
                     /locus_tag="I9760_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAT5044427.1"
                     /translation="MKHRRSETQDIDDAIIKTLNKLLEIELAGVVRYTHYSFMVFGFN
                     RIPICKWMRDQAQESLNHAHLIGELITHFSYHPTLKIGKLLETHEHNISNILEESLEH
                     ERQSLDLYYQLLQYAENKSVLVEEFARQMIQEEEMHLGEISKMLNSPTTFKD"
     gene            complement(2666..3550)
                     /gene="legL6"
                     /locus_tag="I9760_002680"
     CDS             complement(2666..3550)
                     /gene="legL6"
                     /locus_tag="I9760_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL6"
                     /protein_id="HAT5044428.1"
                     /translation="MFYELILTRTSNLIQEFISIPHGVTSLDLSLNELGNISNAELIQ
                     AFHYIPDSVISLDLTNNHLCDKSGAELAQLLAAIPANVTSLDLSSNNLDRRSGAELAQ
                     AFAAIPASVTSLNLHCNYLGNNRGVELAQAFAAIPENVTSLDLSMNYFDLESSADLSQ
                     IFTSIPPHVASLNLSFNSLHEVPFEKLALLNDSLKHVQTVYLSFYSVKEMSKEQRRAL
                     GAAFPNAQKIILIDDYGHEIQPSITISNLIRELSGKADAPSLLNQCILFTQRHQKDSD
                     NKIIPKELEESIRTFNSR"
     gene            3818..5122
                     /gene="sdbC"
                     /locus_tag="I9760_002681"
     CDS             3818..5122
                     /gene="sdbC"
                     /locus_tag="I9760_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbC"
                     /protein_id="HAT5044429.1"
                     /translation="MPNSSIQIWLQRQIALRTFPPNREDWYVKNGFGNSEMSLEERFA
                     SFVEAIRQEDSPYHEQLSGIDFQRFEIEVTLANQKTCMTEVIGFQNNSNQSSSTPNEK
                     FKAVGKGKHVIYFTGIGTQYQDCLIDIAKAVQITGAHYYAFEYPGMRKLGGEVLEVND
                     MVNTGIALANTLLEKGISIDDILFQGDSFGAAVAKKVSDQFKLQSNVTVRCILNNTFS
                     TFQAAVEGNLGAFSTLRYTVRPLLRYTGWDIRPGEGYGQDTPYQIHVNHTGDLTLGAG
                     HATLAESIESNSQLEHFVDPCPEEYRIKRDIYKQWHWASLSEQGITFLETKYGRNSDG
                     QLDTHLADLYLLKYDTGLGVYESFICKYLEDSNDYIASHPQKLSLDELPRPLESETVS
                     LRQLIVSVLPSRPSIAGFFSRSTPVIEEVETEKQEQKSIELK"
     gene            5164..>5554
                     /locus_tag="I9760_002682"
     CDS             5164..>5554
                     /locus_tag="I9760_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAT5044430.1"
                     /translation="MDKRIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPTTDKHTAL
                     IATFGIFAAGFLVRPFSAYWLGNLGDQIGRKKTLTITLMVMTFSSGFIGLLPTYQEAG
                     IFAPILLTFCRLTQGVSVAGEMTLASIY"
BASE COUNT         1625 a         1005 c         1173 g         1751 t
ORIGIN      
        1 tacgcatctc ctcagcccat acggcctctg gatatcgact gcttctttta taatccttat
       61 ctattacgtt agatacatta ttttctctat gggattttga agattacctt tttggggatc
      121 gcaagatgaa aacaggacgt tttgatcaag cccaagctga tgcgtttgct gagcgtcttt
      181 tgtctgttct caatgatgct gcactaactt tgatgatatc cattgggcat cgtaccggct
      241 tgtttgatgt catggacaag cttgattttg caagcagtga agtgatagcc gaggcggcag
      301 gctgcaatga gcgttatgtc agggaatggc ttaatgctct cgtggtggga ggcattgtgg
      361 attatgatgc gcatacaaaa gcctatcaat tacccgctga acatgcttct tttcttacga
      421 gaaaatcttc gccacagaac atggcagtgg tagcgcagtt tattccagtt ttggcctatg
      481 tggaaaatgg catagtggat gcttttaaac acggaggcgg tgttccttat gaggcttatc
      541 atcgttttca tgaagtcatg gcagaagaga gtggacaaac gattttatct tccttgactg
      601 acaaaatttt acctatggtt cacggcttgg ttgacaaact gaaccaggga attcaggtgc
      661 tcgatattgg ttgtggttat gggcgtgcct tgatattaat ggctaaaacc tatcctcaaa
      721 gccaatttac cggttatgat ctgtgtgatg aaaccattgc tcatgctgta cactcagcca
      781 agaaaaataa aatacaaaat atccattttg aaaaaatgga tttgtcggtc tgggatgagc
      841 aggagaaata tgatcttatt accagctttg acgtgattca tgaccaggct aagccagcgc
      901 aggtactgag caacgtattc aaggcattga agcctcatgg tattttcctg atgcaggata
      961 tcaaaacctc cagtgatgtg gcagggaatg ttcatcatcc gctgggggcg cttatttaca
     1021 ctatctcctg tatgcattgt atgactgtat ccctctcaca aaatggctta ggtcttggcg
     1081 cggcctgggg agaggagctt gcagaaaaaa tgctggttga ggctggtttt ggaaaaattg
     1141 aaaagcatta tctggaacat gacattatga atacctacta tgtgatgcgt aaataaaata
     1201 aacagattat gtaagaaatc taatgaggtt aggggctttt cttttaggta acatttactc
     1261 ttgtcagagg ataaaccaat taacggttat ccgttaattg gttaagtaag gatgtcctgt
     1321 taatcaggca tacatcgttt gacggatttc attaaaccgg gcatcatctt tctctttcat
     1381 tgcttcaatg gtttctaatt cctcttgctc atgttcctgt tgagctttga gcttcatgag
     1441 ttcaatcata ttttgcgttt tcaattcttg caaaatagca gctaataaaa tgtctgtggt
     1501 tgtctcaagg ctgatttctt gagtgggttg ttttccttcc tgtggttttt ttgtttccag
     1561 tgttcccgat gagggggtta tttgcttttg actaaaaaaa agtggtgaag cctgattggt
     1621 ataagcttgt ttggtttgac tatctccatt tttgaggtga gtgtttttcg ctttgttact
     1681 catggtgatc tccttttcct tgtgagtccc attcagatta atgcaaatct tgagttaaat
     1741 gcaaatgttt ttttcaatat ttataaattt tatgttttta tcgctaatat agatcattaa
     1801 gatcaggtta tgcccatgtt ttctattaat ccttaagtag taaataaaaa gaacataatt
     1861 ttcttccatc aagccgcctt gaatgataga agtggcaata tattggtaaa attgaataat
     1921 tattttgtgt agccagtttt ttatgtatcc aagcctgcca gatgtcatct ttgtatcagg
     1981 gcttatgtta gatatgagag gcaattctca ataactgata acgcttcgag attttcttga
     2041 aattattcta tacttagata agtaaaatga caggtaatcc tatgaaacat agaagatctg
     2101 aaactcaaga cattgatgat gccatcataa aaactctaaa taaattattg gaaatcgagc
     2161 tagcgggtgt tgtgcgctat actcattact cctttatggt ctttggattt aatcgcatcc
     2221 cgatctgcaa atggatgcga gatcaggctc aagaaagttt gaaccacgct catctaatcg
     2281 gtgagctcat tacccatttt tcctatcatc ctacacttaa aatcggtaag ttattagaga
     2341 ctcatgagca taatatcagt aatattttag aagagtcgct tgagcatgaa aggcaaagtc
     2401 tggatttgta ttatcaacta ttacaatatg cagaaaataa gtcggtgtta gttgaagaat
     2461 ttgcaagaca aatgattcaa gaagaagaaa tgcatcttgg agaaatatcc aaaatgctta
     2521 attccccgac aactttcaaa gattagcaag agttgtttct ctttgtcgat tattccattt
     2581 cttgtttgac tcgaagagtc aaacaagttt ttggtatttt ttaattcatt gacaagagcg
     2641 aacgacggtt gctcaagatt atcaatcagc gagagttaaa agttcgaatg ctctcttcta
     2701 attccttagg gatgatcttg ttatcactgt ctttttgatg tctttgggta aaaagaatac
     2761 attgatttaa caaggatgga gcatctgcct ttccagaaag ctccctaatc aggttagaaa
     2821 ttgttataga cggttgaatt tcatggccat aatcatcaat caaaatgatt ttttgcgcat
     2881 tgggaaatgc agcgcccagt gctcttcgtt gttctttgga catttctttg acactataaa
     2941 agctcaagta aacagtttgc acatgcttta gcgaatcatt aaggagtgcc agcttttcaa
     3001 agggtacttc atgcaaggag ttaaagctca aattaagtga cgccacgtga ggagggatac
     3061 tggtaaaaat ttgtgataag tcagcactac tttccaggtc gaaatagttc atacttaaat
     3121 ccagtgaggt cacattttcg gggatggccg caaaggcttg tgctaactcc actccacggt
     3181 tattgcccag gtaattacaa tgcaaattaa gcgaagttac actggctggg atagcagcaa
     3241 aggcttgtgc taactcagca ccacttcttc tgtcgagatt attagagctt aaatcaagag
     3301 aagtgacatt ggcagggatg gcggccagga gttgtgccaa ttctgcacca cttttgtcgc
     3361 ataagtgatt gttagtcaaa tccaacgata taacgctgtc aggtatataa tgaaaggcct
     3421 gaattaattc agcattactt atattgccta attcatttaa actcaagtca agagaagtaa
     3481 cgccatgagg aatagagata aattcttgta tcaggttgct ggttctggtt aatattaatt
     3541 cgtaaaacat ggtgaaaatc tcccaaatac atagattatg ttttgatcaa aacgaggata
     3601 atatcaacca gttgttcatt attgaaatca atcgtttgta tattgtctat acaaatttct
     3661 tataagcgca gggttaaagt gagtttgttt tgttgcaatc gggtgcaaat cggtgctgtg
     3721 ctgaaaataa gaattatctc taataaagat gcgcaaagaa atgttgactc attagcaaat
     3781 tgtatgcaca cttattgatc aaaaaaaagg gggtgttatg ccgaattcaa gtatacaaat
     3841 ctggttgcaa agacaaatag ctttaagaac ctttcctccc aatagagagg actggtatgt
     3901 caaaaatggt ttcggtaatt cagagatgtc acttgaagaa cgttttgcct cttttgtaga
     3961 agcaatacgt caagaggatt ctccctacca tgaacaatta tccggtatag actttcagcg
     4021 ctttgaaatc gaggtgactt tagccaacca aaaaacctgt atgaccgagg tgataggctt
     4081 tcagaataat tctaatcaat caagctccac acctaatgag aagttcaaag ctgtcgggaa
     4141 gggaaaacat gtgatttatt tcactggaat aggaacacaa tatcaagatt gcctgattga
     4201 tatagcaaaa gctgttcaaa taacgggagc gcattattac gcctttgaat acccgggcat
     4261 gagaaaactg ggcggtgaag tgttagaagt caatgatatg gttaacactg gaatagcgct
     4321 ggccaatacc ttgcttgaaa aaggcatatc aattgatgat atcctttttc aaggcgactc
     4381 ctttggtgct gcagtggcaa aaaaagtcag tgatcaattc aaactgcaat ccaatgtaac
     4441 agtaagatgc attctgaata ataccttttc cacttttcaa gctgctgtag aaggaaatct
     4501 gggtgcgttc tctactctgc gttatacagt tcgtcctctg cttcgttaca cgggttggga
     4561 tattcgtcca ggagagggct atggccagga cacaccctac caaattcatg tcaatcatac
     4621 aggtgatctt accttagggg cagggcatgc aacgcttgca gaaagcattg aatccaattc
     4681 tcaacttgaa catttcgttg atccctgtcc tgaagagtat cgtattaaaa gagatatcta
     4741 taaacagtgg cattgggcct cattaagcga acaaggaata acatttcttg agaccaaata
     4801 tggcagaaac agtgatggac aactggacac gcatttggct gatttgtatt tgctaaagta
     4861 tgatacaggt ctgggggttt atgaatcatt tatttgtaaa tacctcgaag acagtaatga
     4921 ctatattgca agtcacccgc aaaaactttc tcttgacgaa ttacccagac ctcttgagtc
     4981 tgaaacagtt tcactaagac aattgattgt gtctgtgtta ccatcaagac ctagtatagc
     5041 tggtttcttt agtcgtagta cacctgtcat agaggaggtg gagacagaga agcaagagca
     5101 gaaatcaatt gagttaaagt aggctgtacc acttacccgt aaaacaaaaa caacagattg
     5161 gatatggata aaaggattat tgcggcagcc tcttttggta ccttgtttga aatgtacgac
     5221 tatgccattt atgggtttat ggcgcctatt ttggcgccgc tttttttccc cactaccgat
     5281 aaacacactg ctctcatcgc aacatttggt attttcgcgg caggtttcct tgttcggcca
     5341 ttctctgctt attggttagg gaatctgggt gaccaaattg gtcgcaaaaa aacattgacc
     5401 ataactctta tggtcatgac tttttcatca ggttttattg gtttattacc tacttaccaa
     5461 gaagccggta tttttgctcc aattttgctc acgttctgtc gattaactca aggggtttct
     5521 gtggcagggg aaatgacctt agccagtatt taca
//
LOCUS       DACTJE010000187         5529 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.189, whole genome shotgun sequence.
ACCESSION   DACTJE010000187 DACTJE010000000
VERSION     DACTJE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.189"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..590
                     /locus_tag="I9760_002683"
     CDS             <1..590
                     /locus_tag="I9760_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lpg2888 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044431.1"
                     /translation="EVYKAHCSAKEAKEGYNNKIELFKLLLEKNPSLDDNEKKFLFLE
                     IKKLKAETEYQKQMVVFQTMHLLRSVILESFIPAVVFASFVFLPLGIGFAVLGATIGV
                     ALATNLMINAAFKPAKEEVKAFDEKEYEAFCKDPDNWSKPSKSKQGFFQSKEKKPLLD
                     NVAAKTQEKEKDRLELLDDESSIPLLLSSKNNPSI"
     gene            721..2595
                     /gene="mnmG"
                     /locus_tag="I9760_002684"
     CDS             721..2595
                     /gene="mnmG"
                     /locus_tag="I9760_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAT5044432.1"
                     /translation="MNLEQLYDVIVVGGGHAGTEAALAAARLGVKTLLLTHNIDLLGQ
                     MSCNPAIGGIGKGHLVKEIDALDGAMAKAADQAGIQFRILNASKGPAVRATRAQADRV
                     LYRKAIRTQLQSQANLTIFQQAVDDLKIEGGLVTGVVTQMGLTLKARAVVLTVGTFLG
                     GKIHVGMNQYAGGRAGDPPSIALSKSLRDLDLPVGRLKTGTPPRIDRRTIDFSQMVEQ
                     PGDTPVPVFSYLGAASDHPQQVPCHITHTTEATHDIIRNNLDKSPMYAGVIEGVGPRY
                     CPSIEDKIVRFADKTSHQIFVEPEGLTTEEIYPNGISTSLPFEVQVQFVRTIKGFENA
                     HITRPGYAIEYDYFDPRGLTSFLQTKAIPNLFFAGQINGTTGYEEAAAQGIIAGMNAA
                     LQIKDQELWCPRRDEAYIGVLIDDLITCGTQEPYRMFTSRAEYRLLLREDNADLRLTE
                     KGRQLGLVGDERWDSFSKKREAIESTQALLYNSWVRVHHNDLFKETLFNPMQHDCRAV
                     EFLKRPEINYQHLLMMDDLNLPELPQEITEQIEIQNKYAGYIDRQQQEIEKLRKHENT
                     LLPETLDYNDVVGLSSEVIQKLNRIKPTSLAQAGRISGVTPAALSLLLVHLKKSRLPV
                     "
     gene            2592..3218
                     /gene="rsmG"
                     /locus_tag="I9760_002685"
     CDS             2592..3218
                     /gene="rsmG"
                     /locus_tag="I9760_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAT5044433.1"
                     /translation="MIDNTKIRSLLEKGLAEFKLDSIGDLLLDFLLLLNKWNKTYNLT
                     AIRDIETMVSKHLFDSLAILPWIKGNHIIDVGTGPGLPGIPLAIAKPDLQFVLLDSNG
                     KKIRFLNEVKRQLNIKNIEPIQIRVENYHPNQGFDTVISRAFSSLEQMIKWTQHLVAQ
                     DGLWLAMKGRFPDTELVPIHQTYRVERYAVPGIEGERCCVLINNTNKE"
     gene            3221..3991
                     /locus_tag="I9760_002686"
     CDS             3221..3991
                     /locus_tag="I9760_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAT5044434.1"
                     /translation="MAKVIAIANQKGGVGKTTTAINLSASLAASKQQVLLIDLDPQGN
                     ATMGSGVDKRQLVHTTNDVLLRDCLAEQACLATTCGYDLIPGNEDLTVAEVSLMERNH
                     RETFLFKALQPIQSNYDFILIDCPPALNTLTINAFVAADSVLIPMQCEYYALEGLAAL
                     LSTIEQVKSTVNSRLHIEGVLRTMYDARNRLCAEVSKQLLEHFPAKVYRTVVPRNVRL
                     AEAPSHGMPALQYDKSSPGAAAYMVLAAEVISKQTVAS"
     gene            4005..4874
                     /locus_tag="I9760_002687"
     CDS             4005..4874
                     /locus_tag="I9760_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAT5044435.1"
                     /translation="MTVKRSSLGRNLSALLSQSSSSLLANEQPQTEQQLKLAVTCLQP
                     GKYQPRGGMEEAPLNELAQSIKKQGVLQPLLVRELDNGKYEIIAGERRWRASQLAGLT
                     EVPVILKQVDDETAMAMALVENLQREDLNAMDQARAMYRLTNEFALTHQQVADLLCKS
                     RTAVSNFLRLLSLSTPVKKLLENGDIDMGHARALLALDEEQQSQVAQLIIAKNLSVRE
                     TEQLVERVKNGKPELKTHHKDNPMFKEQLNSLAKHLQTKIKLKQGKTGKGTLVIHYEN
                     DHNLQKIIEQLLS"
     gene            complement(4903..5445)
                     /locus_tag="I9760_002688"
     CDS             complement(4903..5445)
                     /locus_tag="I9760_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044436.1"
                     /translation="MKIASNLNSSINIETLVLVATWRFPFVFQGSFKILDRDNYNEHF
                     ILYRCEYTKAHQTDILEDEFSPLDYQFTYLYAGSVKYHDNILNQLSENAIYLLQYALH
                     LTLSKMLTNKRPSDIYIDEIALNFLTERIKQKFDEGKNFESEADIGFAVAMDFNPQEQ
                     TALAAVRNLLSCLTVEVNRV"
BASE COUNT         1745 a         1086 c         1165 g         1533 t
ORIGIN      
        1 tggaagtcta caaagcacat tgctccgcca aggaagcgaa agaaggatat aataacaaga
       61 tcgagctatt taaactctta ttggaaaaaa atccatcact ggatgataat gaaaaaaaat
      121 ttctcttcct ggaaatcaaa aaattaaagg cagaaactga ataccagaag caaatggttg
      181 tttttcaaac catgcatttg ctacgttcgg tgattttgga atcatttata ccagccgttg
      241 tttttgccag cttcgtgttt ttaccgttag gaattggatt tgccgtgttg ggcgccacaa
      301 ttggtgttgc cttggcgacc aacttaatga ttaatgctgc tttcaaacct gcgaaagagg
      361 aagtcaaagc ctttgatgaa aaggaatatg aggctttttg taaagatccg gataattgga
      421 gtaaaccctc caaatcaaaa caagggttct ttcagtccaa agagaaaaaa cctcttttag
      481 ataacgttgc tgcaaaaaca caggaaaaag aaaaggatag gctagagctg ctggatgatg
      541 agagcagtat acctctgttg ttaagtagca aaaacaatcc cagcatataa tcttcattac
      601 atcttgggac aattgccaaa aaagtgtata attgctcatt tttttatatg ttagcttgga
      661 tcttttttgc agatggtcca aaaatccaat ttataatttt ggcataacta atggttaaac
      721 atgaatctcg aacaattata tgatgtcata gtcgttggcg gagggcatgc tggtacagaa
      781 gcagcgcttg cagcagcacg cttgggtgta aaaaccttgt tattgaccca taacatagac
      841 ttgcttggac aaatgtcctg caatccagcc atagggggca ttggtaaagg tcacctggtt
      901 aaagaaattg atgcccttga cggggccatg gctaaagccg ccgatcaggc ggggatccaa
      961 tttcgaattc ttaatgcctc aaaaggacct gccgttcgag ccacacgcgc acaagctgat
     1021 cgtgttttgt atagaaaagc cattcgcaca caattgcaat cacaggctaa cttaaccata
     1081 tttcaacaag cagttgatga tttgaaaatt gaaggcggat tagtcactgg tgttgtaacc
     1141 caaatggggc tcacgctcaa ggcacgcgct gttgtattaa cagtaggaac ttttctgggt
     1201 ggcaagattc atgtcggtat gaaccaatac gcaggaggca gggcaggaga tcctccatcc
     1261 attgcgttat ctaaaagctt gcgggatttg gatttaccgg tagggcgttt gaaaacagga
     1321 actcctcctc gaattgacag acgcaccatt gattttagcc agatggtgga acaaccaggt
     1381 gataccccgg ttccagtatt ttcctatttg ggagcggcaa gcgaccatcc tcaacaagta
     1441 ccatgccata ttacccatac tacagaagcc actcatgaca taataagaaa taatttggat
     1501 aaatcgccaa tgtatgcggg agtcattgaa ggagttggcc ctcgttattg cccctcgata
     1561 gaagacaaaa ttgttcgctt tgctgataaa acatctcacc agatttttgt ggaaccggaa
     1621 ggtctgacaa cggaagaaat ataccctaat ggaatttcta ccagcctccc cttcgaggta
     1681 caagtgcaat ttgtgcgcac cattaaaggt tttgaaaacg cccacatcac caggccaggt
     1741 tacgccatcg agtatgatta ttttgatcca cgcggattaa cctcattttt gcaaaccaaa
     1801 gccattccta atttgttttt tgccgggcaa atcaatggga caaccggata tgaggaagca
     1861 gcagcacaag gaattatagc gggcatgaat gcggcattgc aaatcaaaga tcaagagtta
     1921 tggtgtccgc gcagagatga ggcctacata ggtgtactta tcgatgattt gattacgtgc
     1981 ggaacgcaag agccctatag aatgtttact tcaagagcag agtatcgttt gctattacgt
     2041 gaggataatg ccgatttaag actaactgag aaaggtcgac agcttggctt ggtaggtgat
     2101 gaacgatggg acagcttttc caaaaagcgt gaagcaattg agtccactca agctttgtta
     2161 tacaacagct gggtgagggt tcatcataat gatttgttta aagagacttt gtttaatcca
     2221 atgcaacacg attgccgtgc cgttgaattt ttaaaacgac cggagataaa ttatcagcat
     2281 cttttaatga tggatgattt gaatttgcct gaattgccac aagaaataac agagcaaatt
     2341 gaaattcaga ataaatatgc cgggtacata gacaggcaac aacaagaaat tgagaaatta
     2401 cgcaagcatg aaaacaccct gctcccagag actttggatt ataatgacgt cgttggttta
     2461 tcgagtgagg tcattcaaaa actaaaccgg attaaaccaa cttccttggc tcaagccggg
     2521 cggatttctg gcgtaacacc tgccgcgctg tctcttttac ttgttcatct gaaaaagagt
     2581 cggcttccag tatgatagat aatacgaaaa taagatcatt gcttgaaaag ggtttagccg
     2641 agtttaaact cgattccatc ggcgacctct tgcttgattt tttgctcctg ctcaataaat
     2701 ggaataaaac atataacctc actgcaatac gtgatattga aaccatggtc agcaagcacc
     2761 tgttcgacag tctggctatt ttaccctgga taaagggaaa ccatattatc gatgtgggta
     2821 ctggcccggg tttacctggc atccctttgg ctattgccaa acctgactta caatttgtat
     2881 tattggacag taatgggaaa aaaattcgct ttctcaatga agtaaaacgt caactcaata
     2941 taaaaaacat tgaaccaata caaattcgag tagagaacta ccacccaaac caaggttttg
     3001 atacagtaat aagcagagca tttagcagtc tcgaacaaat gataaaatgg actcaacatc
     3061 ttgtcgctca ggatggtttg tggttagcca tgaaagggcg gtttccggat actgaattgg
     3121 ttccgattca tcaaacatac cgagttgaga ggtatgctgt cccgggtatc gagggagagc
     3181 gttgctgtgt gcttattaac aacacgaaca aggaataatt atggcgaaag tcatagccat
     3241 tgccaaccaa aaaggtggag taggtaaaac aaccacagcc atcaatttat ctgcatctct
     3301 tgccgcatcc aaacagcaag ttttgctgat cgatttagat cctcagggta atgcgacgat
     3361 gggtagtggg gtagataaaa gacagttggt tcacacaacc aatgatgtct tgttacgtga
     3421 ttgcctggct gagcaggcat gccttgcgac aacttgcgga tatgatttaa ttcccggtaa
     3481 cgaagattta acggttgcag aagtcagcct gatggagcgc aatcatcggg aaactttctt
     3541 atttaaagcg ttacaaccca tccagtccaa ttatgatttt attttaattg actgccctcc
     3601 tgctttgaat acattaacca ttaacgcctt tgttgctgct gactctgttt tgatacccat
     3661 gcagtgtgaa tactatgctt tggaaggatt ggctgctttg ttgtcaacca ttgagcaagt
     3721 gaaatcgacg gtaaattcgc gtttgcacat cgaaggcgtg cttcgtacca tgtatgatgc
     3781 acgtaacaga ttatgcgccg aagtttccaa gcagttatta gagcatttcc ctgcaaaagt
     3841 gtatagaacc gttgtgccac gcaatgtaag actggccgaa gcgccaagtc atggaatgcc
     3901 tgccttgcaa tatgataaat cctcaccggg agcagcagct tatatggttc tcgctgccga
     3961 agtgattagt aaacaaactg tagccagtta atttggggta agtaatgaca gtaaaacgca
     4021 gtagtttagg ccgtaattta tctgctttat taagtcaatc aagcagctct ttattagcaa
     4081 atgagcaacc ccagacagaa cagcaactca aactcgcagt gacttgtttg cagccaggca
     4141 agtatcagcc ccgtggagga atggaagaag cccctctcaa tgaacttgcg caatccatta
     4201 aaaagcaagg cgtattacag cccttactgg ttcgtgagtt agataatggc aagtatgaaa
     4261 tcatcgcagg agaaagacgc tggcgtgcaa gccaactggc aggtcttaca gaagttcctg
     4321 ttatccttaa acaagtagat gatgaaacgg ccatggcaat ggcgctggtt gaaaacttgc
     4381 agcgtgaaga tttaaatgcc atggatcagg caagagccat gtatagactc actaatgaat
     4441 ttgctcttac ccatcagcaa gttgccgatc tcttatgtaa atccagaacc gcagtaagca
     4501 attttcttcg tttgctttct ctttcaacgc cagtaaagaa gcttttggaa aatggggaca
     4561 tagacatggg tcatgccaga gcattacttg ctttggatga agagcagcaa agtcaagtcg
     4621 ctcagttgat cattgctaaa aatttatcgg tcagagaaac ggagcaactg gttgagcgag
     4681 taaaaaatgg aaaaccagaa ttaaaaaccc atcacaaaga caatcctatg tttaaagaac
     4741 agttaaactc tttagcaaaa catttacaga ctaaaatcaa attaaaacaa gggaaaaccg
     4801 gtaaaggaac attagttatt cattatgaaa acgatcataa tttgcaaaaa attattgagc
     4861 aattattaag ctaaactttg caatttgcga ttcagtatct atctaaaccc tattcacctc
     4921 aactgtcaaa caggacagta aattgcgcac ggctgcaagt gctgtttgtt cttgcggatt
     4981 aaaatccata gccactgcaa aaccaatatc tgcctcactt tcaaaatttt ttccttcatc
     5041 aaatttttgt ttaatgcgtt ctgttaaaaa gttaagtgca atttcatcga tataaatatc
     5101 acttggcctt ttattagtta gcattttgga caaagtcaaa tgtagggcat actgtaataa
     5161 atatatggca ttttctgata gttgattaag aatattgtca tgatatttaa ctgatcccgc
     5221 ataaaggtag gtaaattgat aatctaaggg agaaaattca tcttccaaaa tatctgtttg
     5281 atgcgctttc gtatattcac acctgtataa gataaaatgt tcattgtaat tatctctatc
     5341 aagtatttta aatgatcctt gaaaaacaaa aggaaacctc catgtagcta ccaacactag
     5401 cgtttctata tttatagaag aatttaaatt acttgctatt ttcatattta aatttcagtt
     5461 gcttctctta ttcccaatat atatgcacta gttagaatac cgaacttagt tagattaaaa
     5521 gcgatctaa
//
LOCUS       DACTJE010000188         5519 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.190, whole genome shotgun sequence.
ACCESSION   DACTJE010000188 DACTJE010000000
VERSION     DACTJE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.190"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            70..159
                     /locus_tag="I9760_002689"
     tRNA            70..159
                     /locus_tag="I9760_002689"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:104..106,aa:Ser,seq:gct)
     gene            166..242
                     /locus_tag="I9760_002690"
     tRNA            166..242
                     /locus_tag="I9760_002690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:200..202,aa:Arg,seq:acg)
     gene            complement(295..1581)
                     /gene="legL2"
                     /locus_tag="I9760_002691"
     CDS             complement(295..1581)
                     /gene="legL2"
                     /locus_tag="I9760_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL2"
                     /protein_id="HAT5044437.1"
                     /translation="MRVELFNPSIKKIIVKLSQLKGLWIVQLNLSKNRLSEKKFTDLA
                     VLLSMLKAFGVTYLNLHFNNLGQKTGTELVALFKILKDSGISRLSLSANELYQKTGTE
                     LATLLTALSDSDIIQLDLSCNWLGQRQDAELIELFKAWSSTPIIDLDLTNNDLGKKSG
                     IVLSEMFRALKVSSISHLGLRNNDLYQRTGAEFSSLLSAIKDSPVTHLDLSDNGLHLM
                     VAAAFETLFDALKDSSVTHLNLCRNGIGHLDMFQMTAMFCSLQCTGVTHLDLGENSLE
                     LMTAEELAAIFQVFKDSRLIHLGLNDSYLNNRTTEDLHLIFNALPANIATIKLSLEEL
                     TEMSSEQRQTIQRRFPRAEQVILVNNMEGRELNPSLSLGDANVYRRLGFVGVSPLSLA
                     GLASFFVASNNLRFAREVQKLLPDEVTERIVKNILG"
     gene            2045..3469
                     /locus_tag="I9760_002692"
     CDS             2045..3469
                     /locus_tag="I9760_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HAT5044438.1"
                     /translation="MIVIFVHGWSVTHTNTYGELPQWLENQSKQGKLDIQVGNIYLGR
                     YISFDDTVTVDDIARAFDQAVRDEIAAKVKDGQRFACITHSTGGPIVRKWMDLYFKNN
                     LAKCPLSHLIMLAPANHGSALAQLGKSRLGRIKSFFEGIEPGKCVLDWLELGSDMSWQ
                     LNESWLDYDCTANGVYSFVLTGQKIDRQLYDAVNSYTGESGSDGVVRVAATNMNYSLL
                     KLHQEGDNGESLVVAKMTRTQPMAFGVLPGLSHSGKNIGIIRSITMANAATHPTAIWV
                     LRCLQVKSRDSYNKLVKELDNITKETQKNEHKEFVKTLVFTREYITNRYSMIIFRLID
                     DRGNHLIDYDLYLTAGPQYSEQALPAGFFVDRQRNLNNRGKLTYFLDYDIMEGGINTP
                     KMQGNLGFRVKAYPESSEQALAYYRLLDFHSSLADIHKILHPNETVMVEIMLQRRVDK
                     TVFRINNNLTPAKISGKPTGKKID"
     gene            complement(3540..4217)
                     /locus_tag="I9760_002693"
     CDS             complement(3540..4217)
                     /locus_tag="I9760_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HAT5044439.1"
                     /translation="MYSYIDFNDKVHRLIPSKFPPVTLFDWADSAEELEQIALLEGLT
                     NERILAELGKINLIDQEDWIGGPGSTPIMAAFTHTGFESRFSDGSFGIYYAASSLETA
                     IKETCFHRERFYSASNEKPCSISMREYVANIKKPLIDITDKSYKDLFNPDPACYSKSQ
                     EFGKKICEKKEWGLLYPSVRDLNGLCVAVLRPPALTIPIQGCHLRYIWDGEKISDIYK
                     ESKITNI"
     gene            complement(4219..4593)
                     /locus_tag="I9760_002694"
     CDS             complement(4219..4593)
                     /locus_tag="I9760_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HAT5044440.1"
                     /translation="MQTNTRSLQSIPDEVVWKSLTNLVERFDLKESEARILMGDMPRS
                     TYTSHKAKLNRDQKERVSYLLGIYKSLRILFEDGEQARTWINRKNDLPPFKGLTPKEY
                     MLEGGMVRLADVRKFLDFWRGY"
     gene            4806..5465
                     /locus_tag="I9760_002695"
     CDS             4806..5465
                     /locus_tag="I9760_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HAT5044441.1"
                     /translation="MIDTIQSEPKINFTYNYKQPDDYHFSLDSIHLAKFVAKQLESYP
                     DLGSLRVLDLCAGCGVIGIELSWYLQAIRQIDFIEIQDIYTECFYQNIANVNRPELQF
                     RWHLLNYDELHKKKWEDKFDLIISNPPYFQPGHGMLSPSKFKNRCRFYLDSSFQSYIQ
                     ALGNSLANRGKAYFLLRPLKHHGLDLFSDIQKILQETSVTATKISHIRGTDIILLEKL
                     K"
BASE COUNT         1731 a         1100 c         1006 g         1682 t
ORIGIN      
        1 ttcatcactt tatgcaataa gaattattga cgcatgttaa atacctgata tactccgcaa
       61 ccattgattg gagagatggc cgagtggtcg aaggcgctcc cctgctaagg gagtatggga
      121 gaaatcccat cgagggttcg aatccctctc tctccgccaa tcaacgcgcc cgtagctcag
      181 ttggatagag tatctggcta cgaaccaggg ggtcggaggt tcgaatcctt ccgggcgcgc
      241 catttttaat cggtctattc cgaaggcatc gcttataaat tcatataaac ctattcagcc
      301 gagaatattc ttgacaatac gttctgttac ttcatccggg agtaactttt gcacttcacg
      361 cgcgaacctg aggttgttgc tggccacaaa aaaggaagct aaaccagcta aagacagggg
      421 agacacccca acaaagccca accgccgata tacattggca tcgcccaagc ttaatgaagg
      481 gtttaactcc ctaccctcca tgttattaac tagaataact tgctccgccc tgggaaaacg
      541 cctttgaatc gtctgacgtt gctcgctgct catctcagtt aactcctcaa gcgataattt
      601 aatcgtcgct atattggctg gcaatgcatt aaaaataaga tgcaaatctt ctgttgttct
      661 gttatttaaa tagctatcat ttaaaccaag atgaattaat ctggagtctt taaagacttg
      721 gaatatagct gctaactcct cagcggtcat caactccaga ctattctcgc ctaaatcaag
      781 atgagtcacc cctgtacact ggagtgaaca aaacatcgct gtcatttgaa acatgtccag
      841 atgcccaatg ccattacggc ataaattgag atgggtgacg gaggagtctt ttagagcatc
      901 aaatagagtc tcaaaagcag cagcaaccat tagatgcaat ccattatcac ttaaatcgag
      961 gtgagtgact ggagaatctt ttatggcact caacagggat gaaaattcgg cacctgtcct
     1021 ttgataaaga tcattattac gtaaaccaag atggctaatt gaagatactt tcaatgcacg
     1081 gaacatttcc gacaacacga tccccgattt tttaccaaga tcattattgg tcaagtcaag
     1141 atcaatgatt ggagtactac tccatgcctt aaatagttcg attagttcag cgtcttgtct
     1201 ttgtcccagc caattgcagc ttaaatcaag ctgaatgata tctgaatcgc ttaaagcggt
     1261 taacaaggtg gctaattccg tcccggtctt ttggtaaagc tcattagcac ttaaactaag
     1321 acggctgatt ccagagtcct taagaatctt aaacagggct accaattcag ttccagtctt
     1381 ttgcccaagg ttattgaaat gcaaattaag ataagtgact ccaaaagcct tgagcatact
     1441 gagcagaact gccaagtcag tgaatttttt ctcactcagt cggttttttg acaaattgag
     1501 ttggacaatc cacaagcctt ttagttgact caatttgaca atgatttttt taatggatgg
     1561 attaaatagt tcaacgcgca tggatgcttc ttatattact ttgcaaggct aaaaaaagcg
     1621 aatacgagct gatttacaaa agctatgtct tttcacctta atctgtccta atctttctcg
     1681 ccgtgcaata ttgtactata tcacccatcc atatccataa tatcttatgg actataattt
     1741 tcaaaacttc aaagccgcat aagcagtcgt tgaatcaact agaaattttg tataattcat
     1801 atataaataa tcgatttcct ttcagttaat taattagatg aaaaatattt caaaagatat
     1861 cattcaagct tatattgctc ttgtctttta acagtaggca aaatattcat taaaaggctg
     1921 cttatttttt aacactgaaa attctgggtc ataattaaaa acttgagggt tgagtataaa
     1981 ataatctaat atcatcaagt tactcaacaa tgtcttgttg tgagccaaca attcaaggag
     2041 cgttatgatt gttatcttcg tccatggctg gagtgtcacg cataccaata cttatgggga
     2101 acttcctcaa tggcttgaaa atcagagtaa acagggaaag ctggatattc aagtcggcaa
     2161 tatttatctt gggcgctata ttagttttga tgatacggtg acggttgacg atatagcacg
     2221 cgcatttgat caagcggtac gtgatgaaat tgctgctaaa gtaaaagatg ggcagcgttt
     2281 tgcttgtatc actcactcta ctggtggacc tattgtccgc aaatggatgg atttatactt
     2341 taaaaataat cttgcaaaat gtccgttgag tcaccttatc atgctggctc ctgccaacca
     2401 cggttctgct cttgctcaac ttggtaaatc ccggctaggc cgcataaaga gtttttttga
     2461 aggcattgaa ccgggaaagt gtgtacttga ttggcttgaa ctcggaagcg atatgagctg
     2521 gcaacttaat gaaagctggc ttgattatga ttgtactgcc aatggtgtct attcctttgt
     2581 acttactggc cagaaaattg atcgccaact ttatgatgca gtgaactcct acacaggaga
     2641 atccggatcc gatggggtgg tacgcgttgc tgcaacaaat atgaattata gcttattgaa
     2701 attacatcag gagggggata acggtgaaag ccttgttgtc gccaaaatga cacgaacaca
     2761 gccaatggca tttggagttc tacctggact ttcacattcc ggtaaaaata tcggtatcat
     2821 ccgtagtatt actatggcca atgcagccac tcaccccacg gcgatatggg tcttgcgatg
     2881 tctgcaagta aaaagtcgtg actcctataa taaattagta aaagagttgg acaatattac
     2941 taaagagact cagaaaaatg agcataagga gtttgtgaaa acacttgtct ttacgcgtga
     3001 gtatataacc aatcgttact ctatgattat tttccggctt attgatgacc gcggcaatca
     3061 ccttatcgat tatgatcttt acctaactgc cggacctcaa tatagcgagc aggcgcttcc
     3121 tgcaggtttt tttgtagatc gtcagcgaaa tctcaataat cgaggtaaac tgacttattt
     3181 tcttgactac gatatcatgg aaggtggaat taatacccca aaaatgcagg gtaatcttgg
     3241 gttccgtgtt aaagcatatc ctgaatcaag tgagcaagcc cttgcctatt acaggttact
     3301 tgattttcat tcatcgcttg ctgacattca caaaatactt catccgaatg aaaccgtaat
     3361 ggttgaaatc atgctgcaaa gacgagtaga taagacagtt ttccgtatca acaacaacct
     3421 tactccggca aaaatcagtg gaaagccaac tgggaaaaag atagattgat acctctttcg
     3481 tttaatgcat ttgcatcatt aatgagaaag tcatctagcc acaatttggg aaaaatatct
     3541 taaatattgg taattttact ttctttataa atatctgaaa ttttttcacc atcccaaata
     3601 tatcttaaat ggcatccttg tatcggtata gtgagcgcgg gaggtcttaa tacagccaca
     3661 cataagccat ttaaatctct tacacttgga tatagcaagc cccattcctt cttttcacaa
     3721 attttcttgc caaattcctg acttttacta taacaagcag gatcaggatt aaacaagtct
     3781 ttataacttt tatctgtaat atctattaat ggttttttta tattagctac atactctctc
     3841 attgagatgg agcaaggttt ttcatttgat gcgctataaa accgttctcg atgaaagcaa
     3901 gtttccttta ttgctgtctc aagagatgaa gcggcataat aaataccaaa actgccatca
     3961 ctaaatcttg attcaaatcc agtatgtgta aaagcagcca taattggagt agatccagga
     4021 ccaccaatcc aatcctcttg atctatcaaa tttattttac ctaactctgc aagtatcctt
     4081 tcattggtta agccttcaag tagcgctatt tgttcgagct cttctgcaga atctgcccaa
     4141 tcgaataaag tcacgggagg gaatttagaa ggtattagtc tatgcacttt atcattaaaa
     4201 tctatatagg aatacatcct aataacctcg ccaaaagtcc agaaacttcc ttacatctgc
     4261 aagacgaacc atcccccctt ccagcatata ttctttgggt gttaaacctt taaacggagg
     4321 taaatcgttt ttacgattaa tccaagttct ggcttgttcc ccatcttcaa acaatatacg
     4381 taaagactta taaattccta aaagatagga gactcgttct ttttgatccc tgtttagttt
     4441 cgctttatgg gaagtgtacg tagagcgtgg catatccccc atcaaaatac gggcttcact
     4501 ttcttttaaa tcaaaacgtt ctacaagatt ggttaaagac ttccatacca cctcatctgg
     4561 tatgctttgt aaagaccttg tattagtttg catactaaac tcctgactgc tttctactat
     4621 agtatagtct ccaaagagac aactgtctac aaaaagacat attaagtgaa ttaaatgctc
     4681 atttaatgaa aaatacattg atctgacatg cagaatatga aataaatttc aaaaatcaat
     4741 taacagtgca acaagcaagg gaataaggtg ctttaaaacc atagttaaga gaaaataatt
     4801 tcaatatgat tgatacaata cagagcgaac caaaaataaa ttttacttat aattacaaac
     4861 aacctgacga ctaccatttc agtcttgatt caattcatct ggctaaattt gttgcaaaac
     4921 aattggaatc ctatccagat cttggttcat taagagtatt agatctctgt gcaggctgtg
     4981 gagtaattgg aattgaactg tcatggtatc ttcaagcaat ccggcagatt gattttattg
     5041 agatccagga tatttatact gaatgttttt atcaaaacat agctaacgtt aatcgacctg
     5101 agctgcaatt tcgctggcat ctcttaaatt atgatgaact acataaaaaa aaatgggaag
     5161 acaaattcga tttgattatc agtaatccac cctatttcca accaggtcac ggaatgcttt
     5221 ccccttcaaa atttaaaaat cggtgcagat tttatttaga tagctctttt cagagttata
     5281 ttcaagcgct gggaaattca cttgcaaaca gaggtaaagc ttatttttta ttacgtccat
     5341 taaaacacca tggtcttgat ttattttctg acatacaaaa aatcctgcag gaaacatcag
     5401 taacagcaac aaaaatatcg catatccgtg gtacagatat tattttattg gaaaagctga
     5461 aatgaatagt agtattcttt ataggacatt gattttggtc tgccttaaaa aataaaaat
//
LOCUS       DACTJE010000189         5387 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.191, whole genome shotgun sequence.
ACCESSION   DACTJE010000189 DACTJE010000000
VERSION     DACTJE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.191"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            253..3129
                     /gene="rapA"
                     /locus_tag="I9760_002696"
     CDS             253..3129
                     /gene="rapA"
                     /locus_tag="I9760_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAT5044442.1"
                     /translation="MTFRIGQRWISNTESQLGLGIITEVDGRQVSISFPAAGEDRIYA
                     IDNAPLSRIVYKTGEEILTNDQQRIKVTSIQEQDGVLLYTGNDDLGNVAQVSELALNC
                     FIKLNSPQQRLFSGLLDKLNAFKLRIKTLEHLGRLQQSKARGLLGSRTNHLIHQIYIA
                     YEVAQRFAPRVMLADEVGLGKTIEAGMILHYQLHTGRASRVLIIVPNSLIHQWFVEML
                     RRFNLHFSIIGPNQYDAMPSTLDELEDNDNPIEPASEDGNLFEGEQLVLTSLDFLMEN
                     ETARQQALAAQWDLLVVDEAHHLYWSEDSQSPEYTFIEKLSARSTGLLLLTATPEQVG
                     MKSHFARLRLLDPARFYDFSAFVNEEKQYHEINKIVQDLMDYKEKNGVDELNPQLHSH
                     LKQLLGKEAPAGINATIKELLDRFGTGRVLFRNTRAAIKGFPERRIHPVALTCPALYS
                     EIIRESNLIHLYPETLLNNDSWIEHDPRVQWLVNKINELRPEKILVICAKAKTAISLE
                     QYLKLRTGIRSTLFHEGLTIIERDRAAAYFAEQENGAQVLVCSEIGSEGRNFQFSHHL
                     VLFDLPLNPDLLEQRIGRLDRIGQNHPIEIHVPYLAGTAQEKLFRWYHEGINILQQSC
                     SVGFSIFEVFENRLTPVLSSPLQDISEQSLEKLIADTQLHTQHIKEALNAGRDRLLEL
                     NSCNYTIGKELIEAIEAEENCLELENYMSQVYQEYGIDQEYHSENAEILRPGNHMKTA
                     HFPGLKEDGMTVTYSRSKALVREDMEFLSWEHPMVYDTMEMILESELGNATVTTISIK
                     SIKPGTLFLETFYTINSSAPKYLQLDRFIPSLPIRILMDTTGKNLSGILSYEQLNNLC
                     QPLKRHLGYPVIKQVREEIESILQQTNKIAEDQMKLFIEEAKSTMELTTSQEVNRLES
                     LQKINPAIRDEEIEFFKKKISESNYFINSSTLKLQAIRVVINVS"
     gene            complement(3243..4646)
                     /gene="ankH"
                     /locus_tag="I9760_002697"
     CDS             complement(3243..4646)
                     /gene="ankH"
                     /locus_tag="I9760_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkH/LegA3"
                     /protein_id="HAT5044443.1"
                     /translation="MSIANDIISHRMPDFDAYLRAGESLDDIDEYGFTPLIECAITRQ
                     IKIAEQLIARKVDINKPDVTGRTPLHWAVDNNDLDMTKLLLTYGADPNAYTRNGLCVL
                     VYPVLRGQDAIKQLLYHHGAKLDFALDFINAKLIGHRFELQGDVDIVNAKGEFIELDY
                     EGFILEFTVAVVKDSLRRFISSYSTRHLRDYFPYIHNIMDAFTDAAELLQYQHHPRLG
                     EQHFKRIAALLKAPMLILPAASRGHALCFVRYHQWWAKIDRGENSLQEGSVNIYRITR
                     PEALTINFIHDFLYKKQNRRYYHQMINQQLGLLPFAQMPISSQISGNCSWANVQAVVP
                     VAYSMQELTSVENFKPDVALSLYDEWVEWDKDRALDECIQRFYLASPERKASIAAILG
                     GILFQACDHANKRHLERAEKILNILILDEYYYVLSSYLEEYCIKRLTRKGNNLLKILD
                     DCGINPNIGVNPVATGL"
     gene            complement(4647..>5387)
                     /locus_tag="I9760_002698"
     CDS             complement(4647..>5387)
                     /locus_tag="I9760_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAT5044444.1"
                     /translation="EEIVLASTVLSYSRPNLSSKSFNDLKNGKTPWEAELDLKGLNVE
                     SARKSLYQFIQTQIKNNKQCVLIIHGADNSQDKPPLMKNLVNRWLPQINEVLAFHSAK
                     PKDGGSAAVYVLLGSVFHVELPTRLPSNRSESTFLVAETKAMERRRRLAEQQGERRFA
                     SVKAREHPEEEIKVAPEGELQNNILQHPELNSQRFDGIDPNLNPEPPLNTEARREFDN
                     ERREQDKEKQLRLGNMPKFTTAPTPRGP"
BASE COUNT         1665 a         1122 c         1017 g         1583 t
ORIGIN      
        1 ttaaaacata cagttcattt tcaattattt aataatgaac gtgagttatg gataagatag
       61 gtcgttatct ccaatcgttc gggctgagga ggcatttatg ccgtctcgaa gccttatatg
      121 gaattttaat gtttcatacc aaggcttcga gacgatgctt aggcatctcc tcagcccgaa
      181 cgaaccctgc atatcgactg cttcttatat aatccttatc cataattcat gttaatgagg
      241 aagtttctct aaatgacatt tcgcattggt caacgctgga taagtaatac cgaatcacaa
      301 ctgggtttgg gcattattac tgaagtagat ggcagacaag tttctatcag tttcccagct
      361 gctggggagg atagaattta tgccatagat aatgcgccat taagccgcat agtctataaa
      421 acaggtgaag aaatacttac caatgatcag caaagaataa aagtaacttc catccaggaa
      481 caagatggtg ttttactgta tacaggaaat gacgacttgg gaaatgtggc tcaagttagt
      541 gagctggcac ttaattgttt tatcaaactt aactcccctc aacaaaggct cttcagcgga
      601 ttattggaca aactaaatgc ttttaaactc agaattaaaa cattagagca tttaggccga
      661 ttacaacagt caaaagcaag aggcctatta ggttcacgga ctaaccacct tatccaccaa
      721 atctatatcg cttatgaagt tgcccaaaga tttgctccca gagtgatgct tgccgacgag
      781 gttggattgg gaaaaaccat tgaagcggga atgattttgc attatcaact gcatactggc
      841 cgtgcctcac gcgtattaat cattgttcca aattcattaa ttcaccaatg gtttgtcgaa
      901 atgttaagga ggttcaatct gcatttctct atcattggtc caaatcaata cgatgccatg
      961 ccatccacac ttgatgagtt ggaagataac gacaacccta tcgagccagc atcagaagat
     1021 ggcaatctgt ttgaaggtga acaactggta ttaaccagcc tggatttcct tatggaaaat
     1081 gaaacggcac gtcaacaggc attagctgcg caatgggatc ttctggtagt agatgaggcg
     1141 catcatcttt attggtcgga agactcacaa agtcccgaat acacgttcat tgaaaaatta
     1201 tcagctcgaa gcacaggcct gcttcttctt actgcgactc ctgaacaagt tggaatgaag
     1261 agtcatttcg ctcgtcttcg tctacttgat ccagcacgct tttatgattt ttcagccttt
     1321 gtaaatgaag aaaagcaata ccatgaaatt aataaaatag ttcaggatct catggattat
     1381 aaggaaaaaa atggcgtaga tgagttaaat ccccaactcc actctcattt aaaacaactc
     1441 ttgggtaaag aagctcctgc tggcattaat gcaacgatca aagaattgct tgatagattt
     1501 ggcacaggtc gagtactttt tcgcaatact cgtgcagcga ttaaaggatt tcccgaaaga
     1561 agaatacacc ctgtcgcatt aacatgccca gcgctttatt ccgagataat cagagaatcc
     1621 aacttaatcc atctttaccc tgaaactttg cttaataatg actcctggat agagcatgac
     1681 ccccgagtgc aatggcttgt taacaaaatt aatgaattac gtccggaaaa aattctggta
     1741 atctgcgcaa aggcaaaaac agccattagt ctggaacagt atttaaaatt aagaacaggc
     1801 attcgaagta ctttatttca tgaaggactt accattatag agcgtgacag agccgccgct
     1861 tattttgcag aacaagaaaa tggtgcacag gttcttgttt gttccgagat aggaagtgag
     1921 ggaagaaact tccagttttc ccatcatttg gttttgtttg atttgccatt aaaccctgac
     1981 cttttagagc agcgtatcgg gcgtttggat cgtattggtc aaaatcatcc cattgaaatt
     2041 catgtaccct atttagcggg taccgcgcag gaaaagctat ttcgctggta tcacgaagga
     2101 atcaacattc ttcaacaaag ctgttctgtt ggcttttcaa tttttgaagt ttttgaaaat
     2161 cggctgacac ctgttttatc cagtccccta caggatatta gcgagcaatc attagagaag
     2221 cttattgctg atactcaatt gcatacccag catattaagg aagcccttaa tgcaggaaga
     2281 gatagattac tagaattaaa ctcctgtaat tacactatag ggaaagaact cattgaagcg
     2341 atagaagcag aagaaaattg ccttgaactt gaaaattaca tgtcgcaagt gtatcaggag
     2401 tatggaattg atcaggagta tcattccgag aatgccgaaa tattgcgccc tggtaaccac
     2461 atgaaaaccg ctcattttcc tgggctaaaa gaagacggca tgaccgtcac ttattctcgc
     2521 tctaaagccc ttgttcggga agacatggag tttttaagtt gggaacaccc catggtttac
     2581 gacactatgg aaatgatcct ggagtcagaa cttggcaacg ccacagtgac tactatttca
     2641 atcaaaagca taaaaccggg taccctcttc ctggaaactt tttataccat taattcttcc
     2701 gcccctaaat acttacaact tgaccgattt attccttcat tgccaattcg aattttgatg
     2761 gatactaccg gtaaaaattt atcaggcatt ttaagttatg agcagcttaa taatctgtgc
     2821 caaccactta aacgtcatct gggttaccct gttattaaac aagttcgtga agagattgaa
     2881 tccattctgc aacaaaccaa caaaatcgca gaagatcaga tgaaattatt tattgaagaa
     2941 gcaaaatcaa cgatggagct aacgacttct caagaagtta atcgactgga atccttgcaa
     3001 aaaattaatc ccgccattcg ggatgaagaa attgagttct ttaaaaagaa aatttcagag
     3061 agtaattact tcattaatag ctccacttta aagcttcaag cgattcgggt tgtaattaat
     3121 gtcagttagt taatcatccc ttgacaaatt gacacgataa taaaagaaat tgcttgtctt
     3181 acaggctgga cgctgatttc acaaaatcaa caactattag attattaacg gaaaatttct
     3241 gtttataggc ctgtcgcaac aggattgacg ccaatattag gattaatccc acaatcatcc
     3301 agaattttta ataaattatt tcctttacgg gttaatcgtt taatacagta ttcttccaga
     3361 taactgctca aaacataata atattcgtct aaaatcaaaa tattaagaat tttctccgct
     3421 ctttccagat gacgcttgtt agcgtgatca caagcctgaa acaaaatgcc tcccaagatt
     3481 gcagcaatac ttgccttacg ttcaggactg gctaaataaa atcgttgtat gcattcatca
     3541 agagccctgt ctttatccca ctcgacccac tcatcatata gtgataaggc cacatcaggt
     3601 ttaaaatttt ctacgctggt cagctcctgc atagaatacg cgactggtac aacagcttgc
     3661 acattagccc acgaacaatt tccactaatt tgagaactaa ttggcatttg agcaaaagga
     3721 agcagcccca attgttgatt aatcatttga tgataatatc gtctgttttg ttttttatat
     3781 aaaaaatcat ggataaaatt gattgttagt gcctctggcc tggttattcg gtaaatatta
     3841 acgcttcctt cttgtaagct attttcacct ctgtcaattt ttgcccacca ctggtgataa
     3901 cggacaaagc ataaggcatg gccacgactc gccgcaggaa gaatcagcat aggggctttt
     3961 aacaaagcag caatacgttt gaaatgctgt tcgccaagcc tgggatgatg ttgatactgc
     4021 aacagctctg ccgcatcagt aaaagcatcc ataatattat gaatatatgg aaaataatcc
     4081 cttaaatgcc gagtcgaata actactgata aatctccgca gagagtcttt tacaacagca
     4141 acagtgaatt ccaaaataaa cccttcataa tcaagttcga tgaattcgcc tttcgcattg
     4201 acaatatcca catcgccttg caattcaaat cgatgaccaa tcaatttggc attaataaaa
     4261 tcaagagcaa aatcaagctt cgcgccatga tgatataaca actgctttat tgcatcttgg
     4321 ccacgcaaaa ccggataaac caacacacaa agaccattac gagtgtaggc attaggatca
     4381 gcgccgtagg ttaataagag tttggtcata tccaaatcat tattgtccac tgcccaatgt
     4441 aatggagtgc gtcctgtgac gtctggcttg ttgatatcca ctttgcgagc aattaactgc
     4501 tctgctattt taatttgtcg tgtaatagca cactctatga ggggagtaaa accatattca
     4561 tcgatatcat ccaaagattc ccctgcacgc agataagcat caaaatcagg cattctatga
     4621 ctgataatat cgtttgcaat actcatttag ggcccccggg gggtaggtgc tgtagtaaat
     4681 tttggcatat tgcctaagcg taattgtttt tctttatcct gttctcttct ctcattatca
     4741 aactcccttc ttgcttctgt attaagcggc ggttcagggt ttaaattagg atctattcca
     4801 tcaaaacgct gactattcaa ttcagggtgt tgcaatatat tgttttgtaa ctccccctcg
     4861 ggagcaacct tgatttcttc ttcagggtgt tcacgcgctt taacagaagc aaaccttctc
     4921 tctccctgct gttcagccaa acgtcttcgt ctttccattg cttttgtttc agccaccagg
     4981 aaagtacttt cactgcgatt ggaaggcaat cgtgtgggta actcaacatg aaacactgag
     5041 cccaacaaga cataaacagc tgcagaacca ccatccttgg gtttggcgct atgaaatgcc
     5101 aatacctcat tgatctgagg caaccaccga ttcaccagat ttttcattaa aggcggtttg
     5161 tcctgagaat tatccgcgcc atgaataatt agaacgcatt gtttgttatt ttttatttga
     5221 gtttggataa attgatataa gctcttacga gcagattcaa cgtttaaacc ttttaaatcc
     5281 agttccgctt cccagggagt ttttccattt tttaaatcat tgaagctttt tgatgataaa
     5341 ttgggtcgag agtaagacaa tacagtactc gcgagcacta tttcttc
//
LOCUS       DACTJE010000190         5369 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.192, whole genome shotgun sequence.
ACCESSION   DACTJE010000190 DACTJE010000000
VERSION     DACTJE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.192"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..589
                     /locus_tag="I9760_002699"
     CDS             <1..589
                     /locus_tag="I9760_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAT5044445.1"
                     /translation="GLPGVSVNWTELVKKLPQSPQLLQMQLQLQAKRKTITAVKKSVW
                     PDLNIQLGGRHFSDDGSNAAVMSAFAEVPVYNRNQGKIMTAEAQYTQTAHEFQGTRLD
                     VRQSVYAAFLQAQQNHYEANLVTDSLLPLARKAIKLAQEGYQMGRYTYIELSTALNSL
                     YEEERHYQQAHADYHKALIQMTGLLGLDNSKKESK"
     gene            586..1839
                     /locus_tag="I9760_002700"
     CDS             586..1839
                     /locus_tag="I9760_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HAT5044446.1"
                     /translation="MTFLKPFKPSILLLILWFVMGVFSWAGEGSHAMEEGNHQEIMEK
                     GPQGGRLFKEGDVALELLIFERGMPPHFRGFVYEKGELIIPTKARLVVYLTRFNGKKE
                     TITFIPVENFLQSNEVIGEPHSFDVTIQLELKGKRLSWHYSTYEGRIKIAPAILKAAD
                     IQTSVAESQTIKTQLKVVGKIAPNRDTLAPIYPRYSGIIKALTKNLGDEVTKGEVLVT
                     IESNESLQNYNITAPITGTIVQKYATNGEQAQNTKPIYEVANLTTVWADFTLYRKETP
                     RVKQGMEVVVTGDEGKPKSTSVISYIAPLGIEDSQTTLARAVLSNEERLWLPGMYVNG
                     AITIKEKTVPVAVLLSAIQRLNDKDMIFVRQGDYFEATPVILGEKDEQWVEVVSGLDV
                     GQPYVSKNSFFMKAELGKEGASHEH"
     gene            1849..5007
                     /locus_tag="I9760_002701"
     CDS             1849..5007
                     /locus_tag="I9760_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HAT5044447.1"
                     /translation="MLEKIIRFSLKHRWFVLLFTAVIAALGVYNFQRLPIDAVPDITN
                     VQVQINTQASGYSPFEVEQRITFPIELAMSGLPSLDYTRSLSRYGLSQVTVVFKDGTN
                     IYFARQLINERLQEVKDKLPPEAETTLGPISTGLGEIFMYTVTNKTNVPASQRYNPME
                     LRTIQDWIIKPQLRNVEGVAEVNTIGGYEKQFHITPDPMKLVRYRLSLNDIVEALERN
                     NANVGAGYIESNGEQHLIRVPGQVKNIADIENIVIASFEGTPVRIRDVAEVALGKELR
                     TGAATENSKEVVLGTVFMLMGENSRTVSERVAAKMKEINKTLPEGVEAVTVYNRTLLV
                     NATINTVKNNLLEGALLVCVILFLFLGNVRAALITAMVIPLSMLLTITGMVTNKISAN
                     LMSLGALDFGLIVDGAVIIVENCIKHLGEQQHALYRVLNLEERLKVIAYATTEVIRPS
                     IFGVFIITVVYLPILTLTGVEGKMFLPMAETVIIALLASMLFALTFVPAAVAIFLRGR
                     VQEKENWLVHYLSLGYARVLRRCFHARFLVISAAVVLVVVSLLVAFRLGGEFIPSLDE
                     GDIAMHAMRIPGTSLTQAITMQHLVEERVSQFPEVKRVFAKLGTAEVATDPMPPNVAD
                     TFIILKPRKDWPNPKKTKQELVQEMEQAVKQIPGNNYEFTQPIQMRFNELISGVRSDV
                     AVKVFGDNMDTLLQVAEAISAQLKKVPGAADVKVEQVSGLPLLTVAINRDVLARYGLQ
                     IGAVQDAIVIATGGKRGGELFEGDKRFDLVVRLPEALRSNPNVLRQVFIPLPPSKDNE
                     RHFIPLSEVATLVRSESPNQISRESGKRRVVVSANVRNRDLSSFVNEAKQRIEQNVKI
                     PTGYWVTWGGQFEQLQSASQRLQIVVPITLLGIFLLLFMSFGRVRDALLVFTGIPLAL
                     TGGVFALWFRGIPLSISAGVGFIALSGVAVLNGLVMITFINKLREQKQVYLKDAVLQG
                     SLARLRPVLMTALVASLGFVPMALATGTGSEVQRPLATVVIGGIISSTFLTLLVLPGL
                     YYVFHERRYRNKLPIKKAGEDT"
     gene            complement(5045..5245)
                     /locus_tag="I9760_002702"
     CDS             complement(5045..5245)
                     /locus_tag="I9760_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044448.1"
                     /translation="MLDWASHNFLITAGIIFSIGLVLIVLAKGSSIHRMKLIANIGFY
                     LAFVALVVFVIVLFLDVLPRKP"
BASE COUNT         1467 a         1066 c         1290 g         1546 t
ORIGIN      
        1 agggttacca ggtgtttcag tcaattggac tgaattagta aaaaaattac cgcaaagccc
       61 tcagctactg caaatgcaac tgcaattgca agccaagcgc aagacgatta ccgccgttaa
      121 aaaatccgtt tggccggatt taaacattca attgggaggt cgtcattttt ctgatgacgg
      181 cagtaatgcg gccgttatgt cagcttttgc agaagtgcca gtatataatc gaaatcaagg
      241 caaaatcatg acagcagaag cgcaatacac ccagacggcc catgaatttc aaggaacgcg
      301 tcttgatgtg cgtcaaagtg tctatgcggc ctttttacaa gcccagcaaa accactatga
      361 agccaatttg gttacggatt ccttattgcc tttggcacgt aaagcgataa aactggctca
      421 agagggctat caaatgggac gttacacgta cattgaatta tcaaccgcac tcaattcttt
      481 gtatgaagaa gagcggcatt accagcaggc tcatgccgat tatcataaag ccttaattca
      541 gatgacagga cttttaggac tggataattc taagaaggag agcaaatgac attcctaaag
      601 ccatttaagc catccattct gcttcttatt ctttggtttg ttatgggggt tttttcctgg
      661 gcaggggagg gctctcatgc tatggaagaa ggcaaccatc aagaaatcat ggaaaaaggc
      721 cctcaaggtg gtcgcttatt caaggaaggt gatgtagccc ttgagctttt gatttttgaa
      781 cggggtatgc ctcctcattt tcgtggcttt gtttatgaga aaggtgaact gattatacca
      841 accaaggccc gtttagttgt ttatctcacc cgatttaatg gtaaaaaaga gaccattacc
      901 ttcattcccg ttgagaattt tttacaaagt aatgaagtga ttggagaacc ccactccttt
      961 gatgtcacga ttcaattgga gctaaagggc aaacgattgt cctggcatta ttccacctat
     1021 gaaggacgaa tcaaaatagc accggctatt ttaaaagcgg ctgatattca aacttcggtt
     1081 gctgaaagcc aaacgattaa aacacaatta aaagtggtcg gaaagattgc ccccaatcgc
     1141 gatacacttg ctccgattta ccctcgttat tctgggatta ttaaagcgtt gactaaaaat
     1201 ttaggcgatg aggtcactaa gggcgaagtg ttggtcacca ttgaaagtaa tgaaagcctg
     1261 caaaattaca atattaccgc acccataacg gggaccattg tacaaaagta tgctaccaat
     1321 ggggagcagg cgcaaaacac gaaacccatt tatgaagtgg ctaatttaac tactgtgtgg
     1381 gctgacttta ccttataccg caaagaaact cctcgggtaa aacaaggaat ggaggttgtt
     1441 gtgacaggag atgagggaaa accaaagtcc accagtgtca tttcttacat tgcgcctttg
     1501 ggaattgaag acagccaaac cactttagca cgtgctgtac tttctaatga agagcgtctc
     1561 tggttgccag gcatgtatgt gaatggcgcg attaccatta aagaaaaaac agttccggtt
     1621 gccgttcttc tttctgcaat acagcgtttg aatgataagg acatgatttt tgtgcggcaa
     1681 ggtgattatt ttgaagcaac acccgtcatt ttaggcgaaa aggatgaaca atgggtggaa
     1741 gtggtgtcag gtcttgatgt tggtcagcct tatgtcagca aaaacagctt ctttatgaag
     1801 gcagaacttg gcaaggaagg cgcaagccac gagcactaag gatggatgat gcttgagaaa
     1861 attattcgtt tttccctaaa acatcgttgg tttgtcttat tatttacggc agtgattgcc
     1921 gctttgggtg tatacaattt tcagcgcttg cctattgatg cggtgcctga tatcaccaac
     1981 gtgcaagtgc aaattaatac ccaggcttct ggctactcac cttttgaggt ggagcagcgc
     2041 atcacctttc ctatcgagtt ggcgatgagc ggcctaccta gtcttgatta tacgcgttct
     2101 ttatcgcgct atggcttatc gcaagtcacc gtggtcttta aagacggcac taacatttat
     2161 tttgcaagac agctcattaa tgagcgtttg caggaagtaa aggataaatt acctcctgag
     2221 gccgaaacaa cgctggggcc gatttcaaca ggtctcggcg agatttttat gtataccgtg
     2281 acgaataaaa cgaatgtgcc tgccagtcaa cgctacaatc ctatggagct tcgcaccatt
     2341 caggattgga taattaagcc acaactgcgc aatgtcgaag gtgttgcgga ggtcaatacc
     2401 attggcggct atgaaaaaca attccacatc acacctgatc caatgaaact cgttcgatat
     2461 cgtttgagtt taaacgatat cgtggaagca ttagagcgca ataatgccaa tgtcggcgcg
     2521 ggttacattg aatccaatgg cgaacaacat ttaatccgtg ttccaggaca ggttaaaaac
     2581 atcgcggaca ttgaaaatat tgtcattgcc agttttgaag gaacgccggt tcgtattcgg
     2641 gatgtagcag aggtggcatt aggtaaggaa ttgcgtacag gggcggctac tgagaacagt
     2701 aaggaagtgg ttttgggtac ggtgtttatg ctgatggggg aaaacagccg cactgtgtct
     2761 gagcgggtgg ctgccaaaat gaaagaaatt aataaaacac tgcctgaagg tgttgaggca
     2821 gtcactgtgt ataatcgaac cttgctagtg aatgccacga ttaatacggt aaagaataat
     2881 ctgctcgaag gggcgctatt agtttgtgtc attctatttt tgttcttagg caacgttcgt
     2941 gccgcactta ttacagccat ggtaattccc ctgtccatgc tgttgaccat taccgggatg
     3001 gtcactaata aaatcagtgc caatttgatg agtttggggg cccttgattt tggtttgatt
     3061 gtcgatggcg cggtcattat cgttgaaaac tgcattaaac atttaggtga acagcagcat
     3121 gcattatatc gtgtgttaaa tttggaagag cgtttaaagg ttattgcgta tgcgaccacc
     3181 gaagtcatta ggcccagtat ttttggagta tttattatta cggtggtgta tttaccaatt
     3241 ttaacgctca ccggtgtgga aggaaaaatg tttttgccta tggctgaaac agtgattatt
     3301 gcgctgctcg cttccatgct gtttgctttg acgtttgttc ccgcagccgt cgctattttt
     3361 ttacgaggtc gtgtgcaaga aaaagaaaac tggttagtgc attacctctc actaggttat
     3421 gccagagtgt tgcgtcgctg ctttcacgcc cgttttcttg taattagtgc cgcggtagtt
     3481 ttagtggtcg ttagtcttct tgttgctttt cgtttaggtg gggaatttat tccgagcctt
     3541 gatgaagggg atattgccat gcatgcgatg cgaattcctg gaacgagctt gactcaagcc
     3601 atcaccatgc agcatttagt ggaggagcgg gtaagccaat ttcctgaagt aaagcgagtc
     3661 tttgcaaaat tagggacagc agaagtagcc accgacccca tgccacctaa tgtggctgat
     3721 acgtttatta tattaaaacc tcgcaaggat tggcctaatc ctaaaaaaac caaacaagaa
     3781 ttagtgcagg aaatggagca agcagtaaaa caaattcctg gtaataatta tgagtttacc
     3841 cagccgattc aaatgcgttt taatgaattg atttcaggag tacgcagtga tgtggccgtc
     3901 aaggtattcg gtgataatat ggatacctta ctgcaagtgg cagaggccat tagtgctcaa
     3961 ttaaaaaaag tacccggggc tgctgatgta aaagtagagc aagtcagtgg cttgccgctt
     4021 ctgaccgttg cgattaatcg tgacgttttg gcgcgctatg ggctacaaat tggtgccgta
     4081 caagatgcta ttgtcattgc aacgggtggc aaaagaggtg gcgagctctt tgagggcgat
     4141 aaacgctttg atttggtagt gcgattgccg gaggccttgc gctcaaaccc aaatgtgttg
     4201 cgccaagtct ttatcccttt acctccttcc aaagacaacg agcggcattt tattcctctg
     4261 agtgaagtgg ctactttagt gcgcagcgaa agccccaatc aaatcagtcg tgaaagtggc
     4321 aaacgtcgtg ttgtagtcag tgcgaacgta cgaaatcgag atttaagttc ttttgtgaat
     4381 gaagcaaaac aacgtattga acaaaacgtc aaaattccca ctggctattg ggtgacttgg
     4441 ggtgggcaat ttgagcagtt gcaatcggcc tcccaacgct tgcaaatcgt agtgcctatc
     4501 actttattag gcattttcct gttgctgttt atgagttttg gcagagtaag ggatgctctt
     4561 ttggtgttta ctggtattcc tttggcatta actggcggag tctttgcttt gtggttccga
     4621 ggcattcctt tgtctatttc agccggtgtt gggtttattg ctttatcagg ggtggctgtg
     4681 ttaaatggtc ttgtcatgat tacctttatt aataaattac gtgagcagaa acaagtgtat
     4741 ttgaaagatg cggtcttaca aggctcgctg gcgaggcttc gtcctgtttt aatgacggcc
     4801 ttggttgcct ccttaggctt tgtaccgatg gccttagcga caggcactgg ttctgaggtg
     4861 cagcgccctt tggcgaccgt agtgattggt ggaatcatct cctctacttt tctcactttg
     4921 ttagtgttgc caggtttgta ttacgtcttt catgagcggc gttaccgtaa caaattacct
     4981 attaaaaaag ccggcgaaga cacttgatta acatggagat gatctctata ttttctcgtt
     5041 ggaattaagg cttcctgggc aagacatcca gaaagaggac tatcacaaag accactaagg
     5101 ccacaaaagc aagataaaat cctatgtttg caatgagttt catgcggtga atagagcttc
     5161 ctttggcaag aactatcaaa accagtccta ttgaaaaaat tatccccgct gtaataagaa
     5221 aattatggct tgcccagtcg agcatcgttg tttctcacga tttgatggta tttacttaag
     5281 tatagatgtc actgggttct tttatgcaag cagtaatgag tggcttcagt gcttattgtt
     5341 ctttgacaat tcctgctaat gaactatat
//
LOCUS       DACTJE010000191         5310 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.193, whole genome shotgun sequence.
ACCESSION   DACTJE010000191 DACTJE010000000
VERSION     DACTJE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.193"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1669
                     /gene="dsbD"
                     /locus_tag="I9760_002703"
     CDS             <1..1669
                     /gene="dsbD"
                     /locus_tag="I9760_002703"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAT5044449.1"
                     /translation="VKKIDPNTFAIQWNILPKYFLYSDRIQLNSDNDDIAQLGTLRFP
                     TPLTKTDKQGRTFKVYRNQLNLPVGVLGITPGETIVNLRFQGCADDGFCYPPEVKQIK
                     LAIDDKLALSQVDLETFHAPEATPLEKPEQDIADIFANHNWIMILLIFYGFGLLLSFT
                     PCILPMVPVLSGIIVGHGKTATTKKAFFLSLSYVLSMSVTYAIVGAVVALLGANLQIS
                     MQSPWAISLFSLIFVLLALSMFGFYEFKLPDAWQSKIVGSSREQRGGHYLGAAIMGCL
                     STLILSPCVTAPLIGVLTYIAQTGNVLLGSVTLFTLSLGMGTPLLLIGTSAGKWLPET
                     GSWMNAVKAFFGILLLAVAIYLMARILPAGLVMGLWACLLIFSGIYSGALTKSNTNQE
                     KLCQGIGIILLTYGLLILIGASMGSSNPLQPLANLQAAPTVSNAFESAKAQSVKSVEL
                     AIKQAFGKPVMLDFYADWCASCKVMENTTFKDPRVQKALSHFIVIKVDVTANNKNDRA
                     LMQHFRVVAPPTFIFFNAEGVQLNNLKRVGELNANEFMQTIKSIDEN"
     gene            1759..3078
                     /gene="rimO"
                     /locus_tag="I9760_002704"
     CDS             1759..3078
                     /gene="rimO"
                     /locus_tag="I9760_002704"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAT5044450.1"
                     /translation="MNHKVGFVSLGCPKALVDSERIITQLKAQGYELVPTYEDAGVVV
                     INTCGFIDSAVQESLDTIKEAMAENGRVIVTGCLGAKADVIKNACPDVLHISGAHAYE
                     EVVNAVHQHLPPPADPFTQLIPPQGIKLTPRHYAYLKISEGCNQKCTFCIIPTMRGKL
                     QSYPMAQILTEAKKLKQAGVKELLVISQDTSAYGVDTRYQQIEWQGKTVNTRFYDLCE
                     QLGELGIWVRLHYVYPYPHVDDIVPLMRDGLILPYLDIPLQHANSRILKAMKRPASSE
                     NTLLRIASWREICPDITLRSTFIVGFPGETEEEFSELLAFLKEAQLDRVGCFKYSPVE
                     GAKANDLDNPVSEDIKEERYHRFMQVQAEISRNKLKNKIGSTQTVLIDEITEDQIIAR
                     SKSDAPEIDGLVYLPKISGITVGSFAEAMITDSDDYDLYGDLEYSLA"
     gene            complement(3148..4266)
                     /locus_tag="I9760_002705"
     CDS             complement(3148..4266)
                     /locus_tag="I9760_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT5044451.1"
                     /translation="MQRLWAIIRKEFLLMKRDPATIVIMAILPLILVCIAGYAINTYP
                     QKVPTVLINLDNTDVTRELVREIQNTGYFSFVASTNDSESAHHLLKTGKAILVLTIPV
                     NFTKALRRNQNPALLLEDGSIDAIASGRAIIALAGLKSSFIEKMGQKGINIPLQRPDF
                     QIINHRLYDPDRVTQFYVVPGMIGLVLMLTMLLITVVVAFRDVQGGTIEYLLASPTRP
                     SEILLGEIFSYILIGYLQLSFGLILSYYLFHVPFIGAPLLLYLCTLPYIVAELSLGLT
                     IATFCTSQFEAVQVINVFIAFSILLTGFTFPIFGMPEWAQYLGLFLPLTHFFKIMFGI
                     MLKGNNFGEIWANLWPLLAYCLTMISLATWRFKRHFVR"
     gene            complement(4281..5207)
                     /locus_tag="I9760_002706"
     CDS             complement(4281..5207)
                     /locus_tag="I9760_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5044452.1"
                     /translation="MSKWSSKQIIIDVSGLAKSFNGINVVVNLNLQIEEGTIFGFLGP
                     NGSGKTTSLRLLSGLIIPDKGQGRCLNLDLMTQTKLIQAQIGYMPQNFCLYQNLTVYE
                     NLDFIARIYQLKNRKERLKEIIELLALSDNQQRITATLSGGWKQRVALAAALLHKPRI
                     LLLDEPTSGIDPQSRLLIWEHIQSIVSKGVTVLLSTQHMDEAERCHQLVYMSAGEIIA
                     RGSVIEIIQSTGLHSWVVKGESFSRLKDILVNNPAIQMIEKGNEMRISSIVDDIHQVL
                     DPFVLNNYEIKKTDTTLEDAFIYKIMKEGNPK"
     gene            complement(5200..>5310)
                     /locus_tag="I9760_002707"
     CDS             complement(5200..>5310)
                     /locus_tag="I9760_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAT5044453.1"
                     /translation="FSEKNRYKLVYKVKADLSQGLRDLLKIGQPVEVNYE"
BASE COUNT         1643 a         1091 c         1006 g         1570 t
ORIGIN      
        1 tgtaaaaaaa atagatccca atacctttgc catacaatgg aatatcttgc ccaagtattt
       61 tttatatagc gaccgtattc aattaaattc cgataatgat gacattgcac agcttggaac
      121 tcttcgcttc cccaccccct taaccaaaac ggataaacaa gggcgaacct ttaaagttta
      181 tcgcaatcag cttaaccttc cagtgggagt cctgggcatt accccaggag aaacaatagt
      241 caatttgcgt tttcaaggat gtgccgacga tggtttttgt tatccccccg aagtaaaaca
      301 aatcaaactg gcaattgacg ataaactggc tctttctcaa gttgatttgg aaacattcca
      361 tgcacctgaa gcaacacctc ttgaaaaacc ggaacaagac attgctgata tttttgcaaa
      421 tcataattgg ataatgattc tgcttatatt ttacggattt ggcctgctcc tgtctttcac
      481 gccttgtata ctgcctatgg tccctgtcct ttcaggtatt attgttggcc atgggaaaac
      541 ggcaacaacg aaaaaagcgt ttttcctgtc attaagctac gttcttagca tgtctgtcac
      601 ttatgctatt gtgggtgctg tcgttgcctt gcttggagcc aatttacaaa tcagtatgca
      661 atcaccttgg gctatcagtc tattcagtct gatattcgtt ttgctggcct tgtccatgtt
      721 tggcttttat gaattcaaac tgccagatgc atggcaaagc aaaattgtag gttcaagtcg
      781 tgaacaacgt ggtggacact atcttggggc agccatcatg ggatgtttgt ccacacttat
      841 tctgtcacca tgtgttacag caccattaat tggagtatta acctacatag cacaaaccgg
      901 aaacgtatta ctcggcagtg tcactctatt tacattaagc ctcggcatgg gtactccatt
      961 actgctgata ggaacttctg ctggtaaatg gctacccgaa acaggaagct ggatgaatgc
     1021 tgttaaagct ttctttggca tattacttct tgctgttgcc atctatctga tggcacgcat
     1081 tttaccagca ggtttggtca tggggctatg ggcttgtctg ctgatctttt ccggcattta
     1141 ctctggtgcc ttaaccaaat cgaataccaa ccaggaaaag ctctgccaag gcattggtat
     1201 cattttatta acctatggat tgctgattct cattggagct agcatgggct cttccaaccc
     1261 tttgcagcct ttagccaact tacaagcagc ccctactgta agcaatgctt ttgaatcggc
     1321 taaagcccaa tctgttaaaa gtgttgaact ggctataaaa caagcgttcg gaaaaccagt
     1381 gatgcttgat ttttatgctg actggtgtgc atcgtgtaaa gtgatggaaa acaccacgtt
     1441 caaagatcct cgtgtacaaa aagctttgag ccattttatc gttatcaaag tggatgtgac
     1501 tgccaataat aaaaatgaca gagcattaat gcagcatttc cgtgtagtag cccccccgac
     1561 ttttatcttt tttaatgctg agggcgtaca actcaataat ttgaaaagag ttggtgaatt
     1621 gaatgccaat gaattcatgc aaacaataaa aagcattgat gagaactaaa gtaactatgg
     1681 gcgcttttgt atgaaatcag tcgacactgg aacgattaag cagtataatt agctcatttt
     1741 ttttacagat agaagtttat gaatcataaa gtaggatttg taagtttagg atgccccaaa
     1801 gcactggtcg attcagagcg aatcattact cagcttaaag cacaaggcta tgaattggtt
     1861 cccacttatg aggatgctgg agtcgtcgta attaatacgt gcggcttcat tgacagcgct
     1921 gtccaggaat cactggatac aattaaagaa gcgatggctg aaaacgggcg agtaatcgtt
     1981 acaggttgtt taggagctaa agcggacgta attaaaaatg cctgtcctga tgtcctgcat
     2041 atcagtggtg ctcatgctta cgaagaagta gtcaatgcgg tacaccaaca cttaccgcct
     2101 cctgcagacc cctttaccca actgattccc ccacagggaa taaaactcac accgcggcat
     2161 tatgcttatt taaaaatatc agaaggatgc aaccaaaaat gcactttttg cattatccct
     2221 acgatgagag gaaaattgca aagctatcca atggcccaaa ttcttactga agctaaaaaa
     2281 ttaaaacaag ccggagttaa agaattactg gttatctcac aagacaccag tgcttatggc
     2341 gtggatacac gttatcagca gatcgaatgg caaggaaaaa cagtcaatac ccgtttttac
     2401 gatctatgcg aacaattagg cgaattagga atttgggtga gactccatta tgtctatccc
     2461 tatccccatg ttgatgatat tgttcctcta atgcgcgatg gactgatttt gccttaccta
     2521 gacataccat tacaacatgc caattcacgc atattaaaag caatgaaaag acctgccagc
     2581 agtgaaaata ccttattgcg gattgcttca tggagagaaa tttgccctga cattacgtta
     2641 cgctccacat tcattgtagg tttccctggt gaaacagaag aggagttttc cgaattacta
     2701 gccttcctta aagaggcaca actagacaga gtcggttgtt tcaaatactc ccctgttgag
     2761 ggagccaaag caaatgattt ggataatcct gtttcggaag atattaaaga agagcgttat
     2821 catcgtttta tgcaagtaca agcagaaatt agccgtaata aattaaaaaa taaaattggc
     2881 agtactcaaa ccgttttaat tgatgagata accgaagatc aaatcatagc tcgcagtaaa
     2941 agcgatgctc ctgaaatcga tggtcttgtt tatttgccta aaatatccgg gattactgtt
     3001 gggtctttcg cagaagcaat gataaccgac agtgacgact atgacttgta tggtgatttg
     3061 gaatactctc ttgcatgaaa gaaatggtat taaaacagta ctttttctgc tcatcttcat
     3121 tggcaagaga gagaatattt attgtatcta gcgtacaaaa tgccttttaa atcgccatgt
     3181 cgctaaagaa atcatggtaa ggcaatacgc gagtagtggc cataaattag cccaaatttc
     3241 accaaaatta tttcctttta acataatgcc aaacataatc ttaaaaaaat gcgtcaatgg
     3301 caaaaataat cctaaatatt gcgcccattc aggcattcca aatatcggaa aagtaaatcc
     3361 tgttaacaaa atagaaaaag caatgaaaac attgatcact tgcactgctt caaactggga
     3421 tgtacaaaaa gtggctatag ttaaacccaa cgataactcc gccacaatat aaggtaaggt
     3481 gcataaatat aaaagaagag gggcgccaat aaacggaacg tggaataagt agtaagacaa
     3541 gataagacca aaacttaatt gtaaataacc aatgagaata taagaaaaaa tttcacctaa
     3601 taaaatttct gatgggcggg tcggtgaggc taataaatat tcaatcgtac cgccctgaac
     3661 gtctctaaac gctacaacca cggttatcag cagcatcgtt aacatgagca ccaagcctat
     3721 catcccggga accacataaa attgagttac gcgatccgga tcatataatc ggtggtttat
     3781 gatttgaaaa tcaggcctct gcaagggaat attaataccc ttttggccca ttttctctat
     3841 aaatgatgat ttcaatcccg ccaaagcaat gatagctcta cctgaagcaa tagcatcgat
     3901 actgccatct tccaataaga gagctggatt ttggtttcgg cgaagtgctt tggtaaagtt
     3961 cacaggaata gtcaatacca ggatagcctt gcctgtcttc aataaatgat gggctgattc
     4021 tgaatcgttc gtcgaagcca caaatgaaaa atacccggta ttttgtatct ctcgaaccag
     4081 ttcacgtgtt acatcagtat tatccaaatt gattaacacc gtaggaactt tttgaggata
     4141 ggtgtttatg gcatatccgg ctatacaaac taaaatcaaa ggtaagattg ccataatcac
     4201 gatagtagca ggatcacgtt tcatcaatag gaattctttg cggatgatag cccacaaacg
     4261 ttgcattgaa aaatgactct tcactttgga ttcccttctt tcataatttt ataaatgaaa
     4321 gcgtcttcta aagtagtatc tgtttttttg atttcataat tgttcaacac gaaaggatcc
     4381 aatacttgat gtatatcatc aactattgag ctgatgcgca tttcattacc cttttcaatc
     4441 atttgaatag cgggattatt tactaatatg tcctttaatc tggaaaaact ctcaccttta
     4501 acaacccagg agtgcaagcc tgtcgattga ataatttcaa tcactgagcc acgtgctatg
     4561 atttctccgg cggacatata aaccaattga tggcatcttt ctgcctcatc catatgctgt
     4621 gtgctgagta aaacagtaac tcctttggat actatacttt gtatatgctc ccaaattaac
     4681 aaacgcgatt gaggatcaat gcctgaagtt ggctcgtcta acaaaagtat acgaggctta
     4741 tgtaacaaag cagccgctaa agccacccgc tgcttccaac ctcctgataa agtagcagta
     4801 atccgttgtt ggttgtcact taaggccagt aattcaatga tttccttcag tctctctttc
     4861 ctgtttttta attgatatat tcttgcgata aaatccagat tttcataaac agtcaggttt
     4921 tgatagaggc aaaaattttg tggcatatag ccaatttgag cctgtatcaa cttggtttgg
     4981 gtcattaaat ccagattcag acatctaccc tggcctttat cgggaattat taatccactt
     5041 aataatcgta aactggtcgt tttaccacta ccgtttggcc ctaaaaaacc aaaaattgtt
     5101 ccttcctcga tttgcaaatt caagttcact actacattga ttccattaaa tgattttgct
     5161 aatccggaca catcgataat aatttgcttt gagctccatt tactcataat tgacttccac
     5221 aggctgacct atttttaata aatcccttaa tccctgagat agatccgcct ttactttgta
     5281 tactaattta taacgatttt tttcactaaa
//
LOCUS       DACTJE010000192         5238 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.194, whole genome shotgun sequence.
ACCESSION   DACTJE010000192 DACTJE010000000
VERSION     DACTJE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.194"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..427
                     /locus_tag="I9760_002708"
     CDS             <1..427
                     /locus_tag="I9760_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044454.1"
                     /translation="SQLVRDAAYSIMSEIYKIEDENLNIVLLLTLESSGHVFFEKVVK
                     QVKKTGEDKNLKYFSSSHLEVELAHALFEEEMERKVYSRRLPISTRREALRMVDRCYD
                     AFSKMFDGLILACNKRLELAKERNQHVAKSVELRENQAV"
     gene            384..1754
                     /locus_tag="I9760_002709"
     CDS             384..1754
                     /locus_tag="I9760_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAT5044455.1"
                     /translation="MSQNQWSLEKIKQYKEDTGQALLSDEQSLVLFGQDFGKLIQSQP
                     VAVSAPQSIESLQSLILFANQYHLPVTIRGNGLSQGGQSLPVPGGLTISMQSFNKPLD
                     LSEDLIWVEANTSWKNLLEKSLLNNKAPYVLPYNCNLSVGGVLSAGGIGASSFKYGVI
                     NAYVSALEVVDGLGRKQIVEKNSPLFQACLSGQGRFGVITKACIQLRSVQPRVKTFFL
                     VYADQNQWFEDIYKIQGKVDYMEIFCSPSIQGARLKEDKRIPMAYWLYGLHLSVEYDR
                     HAENILGQLKPWNVLNIQEESILSYFLRHNSRFDMMKLTGQWDLLHPWYECFVPTSFL
                     TGVLSQLLEELPLHYASLVHVVPIAKQKAGFMMLPDCDSICEFMILNPGVPYSLEESC
                     LKAIENLDKHLLQNNGKRYLSGFLGTELPDNYWLKHFGEKYDSWVGLKKQYDPAGIFS
                     SMLHHI"
     gene            2104..2613
                     /locus_tag="I9760_002710"
     CDS             2104..2613
                     /locus_tag="I9760_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspC"
                     /protein_id="HAT5044456.1"
                     /translation="MKFDIQFLFSANYAKWIIISLISLFGILIIFEWISLFYPLTYVR
                     AIPVSNTKSIAGMREENLNYIFNTSLFGVYVPADLNEDNVKQSMLNVTLVGILFADKI
                     EESQVIIRSASGEEKTYNVGDKIPGGATIKRIMPGGVLVERDGTLESLSLPKNDLTFE
                     PVAKPLKEE"
     gene            2619..2996
                     /locus_tag="I9760_002711"
     CDS             2619..2996
                     /locus_tag="I9760_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAT5044457.1"
                     /translation="MQRIFKLFILIIFIPMMAACIHSTLNLREGIQCFKAQDYRKAFI
                     RLKPEAEKGNPDAQYAVGYMYYYGKGVVEDRKKARFWITCAARAGQPDAIVALGILDR
                     QARRDRELFQDNNLQHFPVDNGL"
     gene            complement(3075..4034)
                     /locus_tag="I9760_002712"
     CDS             complement(3075..4034)
                     /locus_tag="I9760_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAT5044458.1"
                     /translation="MNPILNLIISFIALLWAANHLVAGASGLAIRFQLSPLMIGLTIV
                     ALGTSAPELFISIISSLKDKNDLAIGNAIGSNIANIGLILGIIILIKPTSFHFNKLKK
                     VYPILIIVMLFAYSLILDGYLGTIDGCLFLIGCIAVLCYFIYLASHSPKRDLSVNEFR
                     PAILSYRSTPSNLLSIAIGLLILPICSKYLVYNASEIAKWAGISEFTIGLTIIAIGSS
                     LPELTTSLVAAIKGEDSIAIGTIIGSNIYNLLLILALPGLLNPTKISSVVLWRDMPVM
                     LSITLLLLFLNYYYQKKTSSWPGGILLLVYFCYMASLIINAHS"
     gene            complement(4307..5038)
                     /gene="ceg18"
                     /locus_tag="I9760_002713"
     CDS             complement(4307..5038)
                     /gene="ceg18"
                     /locus_tag="I9760_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg18"
                     /protein_id="HAT5044459.1"
                     /translation="MHLTREVNIMSILSAVSRVINNFSTEIKNRVVEVGSNLKKSTEE
                     FIGEVVIKAAVNRLKQGMLNALSSSNDTTKDNGNDLSTNNPDIEDVSSPTLSTKKKTE
                     ANTTLAGSTGIMQKLLGNIGVNSEQSIEVLSKHFNGAVERFTQDVSKAANEAVCEKIK
                     SIQPEDVIKGINGLIGNTLKVDSESGDTTLSFKLESAVQILGVILAAMGMLMFASQTK
                     STEQKTLEIEHLPTEEIQPPSLAIH"
BASE COUNT         1664 a          854 c         1042 g         1678 t
ORIGIN      
        1 ttcccaattg gtacgtgatg ctgcctattc tattatgtct gaaatttata aaatagagga
       61 tgaaaatctg aatattgtct tgctgttgac cctggaatca tcaggacatg tattttttga
      121 gaaagttgtc aaacaagtta aaaagacggg tgaagataaa aatctgaaat acttctcaag
      181 ttctcatctt gaagtagaat tagcacatgc tttatttgaa gaggaaatgg aaagaaaagt
      241 atattccaga agattaccaa ttagcactcg tagagaagca ctaaggatgg tagatcgttg
      301 ctatgatgca ttcagcaaga tgtttgatgg attgattctt gcttgcaata aacgattgga
      361 attggcaaaa gaaaggaatc aacatgtcgc aaaatcagtg gagcttagag aaaatcaagc
      421 agtataagga agataccgga caagcattgc tcagtgatga gcaatcgctt gttttatttg
      481 gtcaggattt tggcaagctc attcaatcac agccggtagc tgtgtccgcc cctcaaagca
      541 tagagtcatt gcaatcatta attctattcg ccaatcaata tcacttacca gtaactattc
      601 gtggtaatgg tttaagtcaa ggcggccaat cattgcctgt tcctggagga ctaacaattt
      661 ctatgcagtc tttcaacaag ccacttgatt tgagtgaaga tctcatctgg gttgaggcca
      721 ataccagttg gaagaatctg cttgaaaaat cactactgaa caataaagcc ccctatgtgc
      781 tgccttataa ctgtaatttg tcggttgggg gtgttttatc tgcaggagga ataggggctt
      841 cctcttttaa atacggtgtt attaatgctt atgtttcagc attggaagtg gttgatggtt
      901 tggggagaaa gcaaatcgtt gaaaaaaact cacctctatt tcaagcctgc ctttcagggc
      961 aaggtcgatt tggtgtcatt accaaggcat gtattcagct acgatcagtt cagccacgcg
     1021 tcaaaacttt ctttctggtt tatgctgatc agaatcaatg gtttgaagat atttataaaa
     1081 tacagggtaa agtcgattat atggagatat tttgttctcc ttcaattcag ggcgctcgat
     1141 tgaaagaaga taaacgtatt cccatggctt attggttgta tggtttgcac ctttctgttg
     1201 aatatgatcg gcatgcggaa aatattttag ggcagttaaa gccatggaat gtcctgaata
     1261 tacaggaaga gagtatttta tcttattttt taaggcataa ctctcgcttt gatatgatga
     1321 agttgacagg gcaatgggat ttgttacatc cttggtatga atgttttgtt cctacctcat
     1381 ttttaactgg agtactttcc caactgttag aggaattacc gcttcactat gccagtttgg
     1441 ttcatgttgt tccaattgcc aagcaaaaag ccgggtttat gatgttacca gactgcgatt
     1501 caatttgtga atttatgatt ttaaatccag gggtacctta ttccctagag gaaagttgcc
     1561 tgaaggctat tgagaatctc gacaagcact tgctgcaaaa taatggtaaa cgctatttat
     1621 caggattctt aggtacagaa ttaccagaca actattggct aaagcatttt ggagaaaaat
     1681 acgactcatg ggtaggtttg aaaaaacagt atgatcccgc agggattttt tcatcaatgt
     1741 tacatcatat ataaaaaata gtattgtgca aactcatgct tcgtaacaga tatcaaggca
     1801 tgatggtcga tctgatgcat aaaggtatga attcagcctg gataaagata tattatctac
     1861 agggcttacg aaacccccta tctctttgtt aaaaaaagtt tttaattcgg tcatttagtt
     1921 tattctaagc tccttcatta aaaacatttt ttgccttgac cttgggatat tttgtaagct
     1981 ctgatctagt gatcttgaag tgagggtgat aaagatctat tttgtggtag gatgctatat
     2041 atcaatatta tttaaagaga aggatgaatt tgttttgata ggtttaactg ggattggaaa
     2101 cgtatgaaat ttgatattca atttttgttt tcagccaatt acgctaaatg gataatcatt
     2161 agtttaatat ccttgtttgg tattttgatt atttttgaat ggatttcctt attttatcca
     2221 ttgacttatg ttagggctat ccctgtttca aatacaaaat ctattgctgg aatgagagaa
     2281 gagaatctca attatatttt caatacgtcc ttatttggag tttatgttcc tgcagactta
     2341 aatgaggata atgtaaaaca atcaatgctg aatgttactt tggtcggtat tctttttgcg
     2401 gacaagatag aagagtctca agtgattata cgatctgcca gtggggaaga aaaaacctat
     2461 aatgtaggag ataagatacc tggtggtgca acaattaaac gtattatgcc aggaggagtg
     2521 cttgtggaaa gagatggcac tttagaaagt ttaagtcttc cgaaaaatga tttaaccttt
     2581 gaacctgtcg ctaagccttt aaaagaggaa taaatggcat gcaacgcatc tttaagttat
     2641 tcattctaat aatttttatt cctatgatgg ctgcctgtat tcatagtacc ttaaatttac
     2701 gagaaggaat ccaatgcttt aaagcacagg attaccggaa ggcattcatt cgtttaaaac
     2761 cggaagctga aaagggaaat ccagatgctc agtacgctgt aggttatatg tattattatg
     2821 gtaaaggtgt ggtagaagat agaaaaaaag cccgtttctg gataacctgt gctgcaagag
     2881 caggtcagcc agatgccata gttgctcttg gaattcttga tcgccaagca aggcgggata
     2941 gagaactatt tcaagataat aatttgcaac attttcccgt tgataatggt ttataagttt
     3001 tttactttaa gctaatttct ttgattcaaa tgctttctta aaaacccggg taacccaggc
     3061 caggttagtt ttagctaact gtgtgcgtta ataatcaaag aagccatgta acaaaaatac
     3121 acaagcaaaa gtatcccccc aggccaggag gatgtctttt tttgataata gtaatttaaa
     3181 aacaataaaa gtaaggtaat ggaaagcatc actggcatat cacgccataa tacgacgctg
     3241 ctaattttag ttgggtttag taatcccggt agagctaaga ttaacaacag gttataaata
     3301 tttgagccaa ttatcgtacc tatcgctata gagtcttctc ctttgatagc ggcaacaagg
     3361 gacgttgtaa gttctggcaa ggaggaacct atagcaataa tggtcaagcc aatggtgaat
     3421 tcacttatac cagcccattt tgcaatttca gatgcattat aaaccaggta ttttgaacaa
     3481 ataggcagaa tcaataaacc tattgcgata ctcaataaat tagagggtgt tgaacgatag
     3541 gatagtatcg cgggtctaaa ttcattaact gataaatctc ttttaggcga gtgactagct
     3601 aaataaatga aataacagag tactgctata catccgatca aaaaaaggca accatcaatt
     3661 gtacccaaat aaccgtcaag tattaagctg tacgcaaaca gcatgacaat tatcaatatt
     3721 gggtatactt tttttagttt attgaaatga aaagaggttg gtttgataag gattataatc
     3781 cctaaaatta agccaatatt tgctatattt gagccaatcg catttccaat tgccaggtca
     3841 tttttatctt ttaaggaaga aatgattgag ataaataatt cgggggcact ggtacccaat
     3901 gcaacaatag ttaaaccaat cataaggggg gataattgaa aacgaattgc cagccctgag
     3961 gcaccagcaa ccaaatgatt ggcagcccaa agaagggcaa taaaactgat aatcaaattc
     4021 agaatagggt tcatttttgt actcgatatt ttttagtgat aataatacga gaatggttta
     4081 taggatataa cttatagata ttaagtgtca ctttatattc aggttttgcc aggagctcat
     4141 gtaatcatca tgttaattaa aaaatgcttg tatatcatga agcggggcga gttcagtgat
     4201 caaacgttgt aattaaggag tttatgaatg tgttgccaat tcttcaagct acataccaag
     4261 aatgatttgc ggcatgcagc cttgaagaac aagccgtgag catttattaa tgaatagcca
     4321 atgaaggtgg ttgaatttct tcagttggca gatgttctat ctctagagtt ttctgctcgg
     4381 ttgatttagt ttgagatgca aacatcagca tacccatggc tgcaagaatg acgcccagga
     4441 tttgtacggc actctcaagc ttgaatgata gggtagtgtc gccggactct gagtctactt
     4501 tgagagtatt gccgattaga ccgttaatac cctttatcac atcttcaggt tgtatacttt
     4561 tgatcttttc acacacagcc tcatttgcag cctttgaaac atcctgggta aaacgctcaa
     4621 cagcaccatt aaaatgcttg gacaatacct ctatactttg ttcagaattt acgccaatat
     4681 tacccaacaa tttctgcatt ataccggtac tgccagccag agtggtattt gcctcagttt
     4741 ttttctttgt gcttaaagta gggctagata catcttcaat gtcagggttg tttgtggata
     4801 aatcattacc attatctttg gtggtgtcat tggaagaaga caaagcattc aacattcctt
     4861 gtttcaaacg attaacagca gcttttatga ctacttcgcc aataaactct tctgttgact
     4921 tctttaaatt actccccact tcaacaactc tatttttaat ttcagttgaa aaattattta
     4981 tgacacgtga aacagcagat aatattgaca taatgtttac ctctcttgtt aagtgcaaat
     5041 aaataataag aagagaacat taagtgaaca ttaagaaatt gttaagattg aaacatttct
     5101 ttacaaaaaa atgtaaatta aaagtaattg ttcattattt attaaataat gaataaataa
     5161 attatttgta aagattttgt tagattgata ttggcttatg cggacttaac agtttataca
     5221 ccttttggtg gttttaaa
//
LOCUS       DACTJE010000193         5235 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.195, whole genome shotgun sequence.
ACCESSION   DACTJE010000193 DACTJE010000000
VERSION     DACTJE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.195"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(101..2218)
                     /locus_tag="I9760_002714"
     CDS             complement(101..2218)
                     /locus_tag="I9760_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HAT5044460.1"
                     /translation="MFRFLFPVSLFISSILLFSIQPMVAKAILPIYGGTPGVWTVCVL
                     FFQFILLIAYGYVWLLSQIKKPAVWRLIHMVLVVLSFTALPLLFHPAARDGQPEWVIL
                     HNLLIQLGLPLLVIGASAPLLQFAYSQTKSKGASDPYYLYISSNLGSLSALLFYPWVI
                     ERFIGLTRQFYLWNFGFAIYLLLLVTVLFFTKYQPLILTEKKEVDFLPWREIAYWIFL
                     SFIPCSLMLGVTLYITTDVAATPLFWVLPLALYLLSFVFTFTATPLISQKWISRNCLF
                     FLVFTILGFIFGVSQIRVWQLVLFNLLSFFILALLCHGQLFARRPKPHKLTLFYFCLS
                     IGGVLAGIFNGILAPNWFNYVYEYPLAILLSLFALQLPKTNRGWWVPLVVLGLLSLHY
                     FIPTVPWFKSTTTFHILAILALGIIVIWHRNKINLVLSMLILFVFLYFPPLQDKQILL
                     KERNFYGVKQVFKKDDVHALISQSTLHGMQVMNGEKASYGRSSYYGAIAPIVDILKKE
                     FHPLSATIMGLGAGTLLCQFRATDSIKVIEIDQQVIDLAKNNKLFTYIRDCSPSAEII
                     KQDGRLALVNMPDASQDLLILDAFNSDAIPVHLMTLEAFTLYKKKIVEDGVILVNLSN
                     RHLQMLPVINAIGRALNMITLFLAHKGDKKLGQFNSEWAMLTSNQSLAFQVMKGTNWK
                     FVADDDQFLWTDDYSNIIPLLKW"
     gene            complement(2475..2915)
                     /locus_tag="I9760_002715"
     CDS             complement(2475..2915)
                     /locus_tag="I9760_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAT5044461.1"
                     /translation="MSKVIKKLEVALADTYALYLKTQNYHWHVTGPQFKSLHELFEMQ
                     YKELAEAVDQIAERIRIMGHKAPATFAEFNQLKTIKDGDSGLSANEMVSELAKDNMMI
                     VKDLNQAIKIAQENEDEGTVTILSDRVAAHEKAHWMLSASKEKK"
     gene            complement(3038..4684)
                     /gene="groL"
                     /locus_tag="I9760_002716"
     CDS             complement(3038..4684)
                     /gene="groL"
                     /locus_tag="I9760_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234810.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAT5044462.1"
                     /translation="MAKELRFGDDARLQMLAGVNALADAVQVTMGPRGRNVVLEKSYG
                     APTVTKDGVSVAKEIEFEHRFMNMGAQMVKEVASKTSDTAGDGTTTATVLARSILVEG
                     HKAVAAGMNPMDLKRGIDKAVLAVTKKLQAMSKPCKDSKAIAQVGTISANSDEAIGAI
                     IAEAMEKVGKEGVITVEDGNGLENELSVVEGMQFDRGYISPYFINNQQNMSCELEHPF
                     ILLVDKKVSSIREMLSVLEGVAKSGRPLLIIAEDVEGEALATLVVNNMRGIVKVCAVK
                     APGFGDRRKAMLQDIAILTKGQVISEEIGKSLEGATLEDLGSAKRIVVTKENTTIIDG
                     EGKATEINARIAQIRAQMEETTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKK
                     ARVEDALHATRAAVEEGIVAGGGVALIRAQKALDSLKGDNDDQNMGINILRRAIESPM
                     RQIVTNAGYEASVVVNKVAEHKDNYGFNAATGEYGDMVEMGILDPTKVTRMALQNAAS
                     VASLMLTTECMVADLPKKEEGVGAGDMGGMGGMGGMGGMM"
     gene            complement(4712..5002)
                     /locus_tag="I9760_002717"
     CDS             complement(4712..5002)
                     /locus_tag="I9760_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAT5044463.1"
                     /translation="MKIRPLHDRVVVRRMEEERTTAGGIVIPDSATEKPMRGEIIAVG
                     AGKVLENGDVRALAVKVGDVVLFGKYSGTEVKVDGKELVVMREDDIMGVIEK"
BASE COUNT         1732 a         1159 c          847 g         1497 t
ORIGIN      
        1 taatattcct taattacata ccccatgaac agttactatg gatagcccgc atcacgataa
       61 agcaaggata caattgaatt tgatagctta cctttttacc ctaccatttc agcaagggga
      121 taatattaga ataatcatca gtccataaaa actgatcatc atctgctaca aatttccagt
      181 ttgttccttt catcacttga aacgccaagg attgatttga ggtcaacata gcccactctg
      241 aattaaattg ccccaacttc ttatctccct tatgcgctaa aaataaagtt atcatattca
      301 aagctcgccc aattgcatta attaccggga gcatttgcag atgtctgtta cttagattaa
      361 ccaaaataac tccatcttcg acaattttct tcttatataa ggtgaaagct tcaagtgtca
      421 tcaaatggac aggaatagca tctgaattaa acgcatccaa aattaacaaa tcctgtgaag
      481 catctggcat attaaccaag gccaatcgtc catcctgttt aataatctca gctgaaggcg
      541 aacagtctcg tatgtatgtg aataatttat tatttttagc taaatcaatt acttgttgat
      601 caatctcaat aacctttatg gaatcagttg cccgaaattg gcacagtagt gttcctgcac
      661 ccaaccccat aatcgtcgca gaaagcggat gaaattcctt cttcaaaata tcaactattg
      721 gagctatggc cccataatag gagcttcttc cataacttgc tttttcccca ttcattacct
      781 gcatcccatg aagggttgat tggcttatta atgcatgcac atcatccttt ttgaagactt
      841 gcttgacgcc gtaaaaattt ctttccttga gtaaaatctg tttgtcttgc aacggaggaa
      901 agtaaaggaa aacaaataaa atgagcatgg atagtacaag attgatttta ttacgatgcc
      961 aaatcacaat aattccaagt gccaaaatag caaggatgtg aaaagtggta gtacttttaa
     1021 accagggaac ggtaggaatg aaataatgaa gcgacagcaa acccaaaaca actaatggca
     1081 cccaccatcc tcggttagtt ttcggcagct gaagagcaaa taaacttaat aaaatggcta
     1141 atgggtattc ataaacataa ttaaaccaat ttggcgctaa aattccatta aaaatccctg
     1201 ctaaaactcc tccgatagac aaacaaaaat aaaacaaagt gagtttatgt ggcttgggtc
     1261 ttcgcgcaaa taactggcca tgacacagta aagccaaaat aaaaaaactt aacagattaa
     1321 ataacaccaa ttgccacact ctgatttggc taactccaaa aatgaatcca agaattgtaa
     1381 aaaccaggaa aaacaggcaa ttacgagaaa tccatttttg tgaaataagg ggggtcgctg
     1441 tgaaggtaaa tacaaatgac aaaaggtaaa gcgctaaagg caaaacccaa aataagggag
     1501 ttgcagcaac atcagttgta atatataaag taactcccag catcaaactg caagggataa
     1561 aactcaaaaa aatccaatag gctatctctc gccaaggcaa aaaatccacc tctttttttt
     1621 cggttaatat taaaggttgg tattttgtaa aaaacaagac tgttaccaac agtaacaaat
     1681 aaatagcaaa tccaaagttc cataaataaa attgacgagt caaaccaata aatcgctcaa
     1741 taacccatgg ataaaaaagc aaggcactca agcttccaag attactggat atgtacaaat
     1801 aataagggtc tgaggcccct ttgctttttg tctggctata ggcaaattgt aataaaggag
     1861 cagaagcacc aatcactaac aaaggcaaac ccagttgaat taacaaatta tgcagaatca
     1921 cccattcagg ttgtccatcc ctggctgcag gatgaaacag caatggaagt gctgtaaaac
     1981 ttaaaacaac caaaaccata tggatcaaac gccaaacagc aggtttctta atctgactta
     2041 aaagccatac gtagccatat gcaatcagca gaataaattg aaaaaaaagc acgcaaacag
     2101 tccacacacc aggtgttccc ccataaattg gaagtattgc ttttgcaacc atgggttgaa
     2161 tactgaatag gagaatggaa cttataaaaa gactgacagg aaataaaaat cgaaacacaa
     2221 agcaatcctt agccgatggt gctgccaaat gaatgaatcg atcattttac gactacactc
     2281 tcgttcatac aaggatgaat tattactctg caaaacaatt tataaacata attgataggg
     2341 atgtttcaca aacacatatt attccatatt gactgatgga aaaatccatt tatggttttt
     2401 gaagcttttg taaacatccc attacttgaa gataggcact gattccatat caactgatac
     2461 ataaaatgaa tactttattt cttttccttg gaggctgata acatccaatg agctttttca
     2521 tgagccgcaa ctcgatcgct tagaatagtc acagtacctt cgtcttcatt ttcctgggca
     2581 attttaatcg cctgatttaa atcttttact atcatcatat tatcttttgc caactcactg
     2641 accatctcat tagcactcaa tccggaatca ccatctttaa tcgttttcaa ttgattaaac
     2701 tcagcaaagg tagcaggagc cttatgcccc atgattcgaa ttcgctctgc aatttgatcc
     2761 actgcttccg caagttcttt atattgcatc tcaaataatt catgcaaact tttaaattga
     2821 ggtccagtaa catgccaatg gtaattttgt gttttcaggt acaacgcata agtatcagca
     2881 agagcgactt ccagcttttt aattacttta ctcattattc tttccttgtt atttattgac
     2941 gttattaagg atagttcaaa acagtcaaag aacaaaatag acattaataa aaaacccgcc
     3001 tttacagcgg gttttttatt ggataaccgg gagaaaatta catcattccg cccatgccac
     3061 ccattccgcc catgccgccc atatcgccgg caccaacacc ttcttctttc ttaggcagat
     3121 cagcaaccat acattcagta gtcaacatca aactggctac agaagctgca ttttgcagag
     3181 ccatacgggt tactttggtt ggatcaagaa tacccatctc aaccatatca ccgtattcac
     3241 cagttgcagc gttgaaaccg tagttgtctt tgtgctcagc taccttgttt actacaacag
     3301 aagcttcata tcctgcgtta gtaacaatct gacgcattgg agattcaata gcgcgacgta
     3361 aaatattgat acccatattt tgatcgtcat tatcgccttt caatgaatca agagctttct
     3421 gagcacgaat caaggcaacg ccaccaccgg caacgatacc ttcttctact gcagcgcgag
     3481 tagcatgaag agcatcttca acacgagctt tcttctcttt catttcaact tctgtggcag
     3541 cgccaacttt gataacagca acaccaccag ctaacttagc aacgcgctct tgtaattttt
     3601 ctctatcgta atcagaagtg gtttcttcca tttgtgcacg aatttgagca atacgagcat
     3661 taatttcagt tgcctttcct tcaccatcaa tgatagtagt gttttctttg gtaacaacga
     3721 ttcgcttagc actaccaaga tcttccagag tagcaccttc caggctcttg ccaatttctt
     3781 cagaaatcac ttgaccctta gtcaaaatag caatgtcttg caacatcgct ttgcgacgat
     3841 caccaaaacc aggcgctttg acagcacata cttttacaat accgcgcatg ttgttaacta
     3901 ccagagtagc taaagcttcg ccttcaacat cttctgcgat gatcaataaa ggacggccag
     3961 attttgcaac accttccaat acggacaaca tttcacgaat actggaaact tttttgtcaa
     4021 ccaataaaat gaatggatgc tcaagttcac agctcatgtt ttgctggttg ttgatgaagt
     4081 atggagaaat gtaaccgcga tcaaattgca taccttcaac aacagaaagc tcattttcca
     4141 atccattacc atcttcaacg gtaataacac cctctttacc aactttttcc attgcttcag
     4201 caatgatagc accaatcgct tcatcggaat tagcagaaat agttccaact tgagcaatag
     4261 ctttgctgtc tttgcatggc ttagacatag cttgtaattt tttggtaact gctaatactg
     4321 ctttatcaat accgcgtttg agatccattg gattcatacc agcagcaact gctttgtgac
     4381 cttcaacaag aatagaacga gccaatactg ttgcagtagt agtaccatca ccagcagtat
     4441 cagaagtttt agaagccact tctttaacca tttgagcgcc catgttcatg aaacgatgct
     4501 caaattcaat ttctttggca acagacacac cgtctttagt tacagtagga gcgccataag
     4561 atttttccaa tacaacatta cggccacgtg gacccatagt aacttgaacc gcatctgcta
     4621 atgcattaac accagcaagc atttgtaggc gagcgtcatc accaaaacgt aattctttag
     4681 ccattatcat tgtctccttt aaacaattag attacttctc gattacaccc atgatgtcgt
     4741 cttcgcgcat cacaactaat tcttttccat caactttaac ttcagtacca gagtacttgc
     4801 caaacaatac cacatcacct actttaactg ctaaagcacg aacatcaccg ttttccaata
     4861 ctttgccagc gccgacagca atgatctcac cacgcatagg tttctcggta gcactatctg
     4921 gaataacaat accaccagca gtggtacgct cttcttccat acgacgaaca acaacgcgat
     4981 cgtgtaaagg acgaattttc atacttttct ctcctgatta aattaaactt taaatcattt
     5041 aagtattgct ttccttaggg caaacaatac cgatgacagc gatatgggga caataaacaa
     5101 actttcaagg gtaaaatcaa aaaaaatttt ggatttggga ttacagagtc tacaataatg
     5161 tttattagta actgtaaaag atatcgaatg aaaaagtggc tcttattttc cctattattt
     5221 ttaacttccc tttgt
//
LOCUS       DACTJE010000194         5204 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.196, whole genome shotgun sequence.
ACCESSION   DACTJE010000194 DACTJE010000000
VERSION     DACTJE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.196"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..824
                     /locus_tag="I9760_002718"
     CDS             <1..824
                     /locus_tag="I9760_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAT5044464.1"
                     /translation="ENIIDIKAAARDPGNRAKIAVKTNDGRIDPIGACVGMRGARVQA
                     VSSELGGERVDIILWDDNPAQLVINAMAPADITSIVVDEDTHTMDLAVAKEQLSQAIG
                     RNGQNVRLASQLTGWTLNVMTTEEFESKNQEESSKIVNLFTSALEIDEEIATLLVAHG
                     FSSLEEVAYVPKEELLAIEEFDEEIVEELRNRANDKLLEMALSSGKGLSGTPDDSLIN
                     MEGMTEDLANKLASKGITTMEDLAEQSVDELMEIEGISEEKAGALIMKAREPWFQ"
     gene            913..3519
                     /gene="infB"
                     /locus_tag="I9760_002719"
     CDS             913..3519
                     /gene="infB"
                     /locus_tag="I9760_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAT5044465.1"
                     /translation="MADVTVKQLAQVVGIPVERLLNQLQEAGLSFTDDQQTVNEEQKR
                     ILLNHLKGSSNRDISAAPERITLRRKSMSQVTVGHDMHSGKTVNIEVRKKKTFIKRSA
                     IPEQAEVEEPVVPPVVEEPVHEEITVVSEVSAETPELKETEEHPVIEPVAELDETVKE
                     EEKINSEENTAESQDELTHANTDVIENLVDVVEETIPVSKKEEVKPEKVSKKKHLEQT
                     DSDISEFKKGKKKPKYHTFEHDEEEQELHRRGGRSKFKKKKGTEKSDKYREAEETLTH
                     GFALPTAPIVREVLIPETITVAELAKRMSVKAAEVIKVMMSLGAMATINQVIDQETSV
                     IVVEEMGHKPIIIKEDAVETGLGEAISKGTKTEGRAPVVTIMGHVDHGKTSLLDYIRR
                     TKVAAGEAGGITQHIGAYHVSTPKGNITFLDTPGHAAFTAMRARGAQATDIVILIVAA
                     DDGVKPQTIEAIQHAKAAKVPIIVAINKMDKPDADPERVMNELSVQEVIPEAWGGDTM
                     FVNISAKSGMGIDDLLDAILLQSEVLELKAVTDGAAKGVVIESRLDKGRGPVATVLVQ
                     SGTLHKGDILLAGFQYGRVRALVSDNGDLVDSAGPSIPVEVLGLSAIPHAGDEAVVVP
                     DEKKAREVALFRQGRFRDVKLARRQKTTIEGIMENMTATESKVLNIVLKADVQGSLEA
                     ISDALTKLSTDEVKVDVISSGVGGITESDVHLAIASNAILIGFNVRADGTAKRLAEQE
                     SVSIHYYSVIYDIVDQIKGALTGMLAPQFKEEIVGIAEVRDVFKSPKIGAIAGCMVIE
                     GVVKRNNPIRVLRSNVVIYEGTLESLRRFKDDVLEVRQGFECGIGVKNYNDVKPGDLI
                     EVFETVEIKRDL"
     gene            3523..3894
                     /gene="rbfA"
                     /locus_tag="I9760_002720"
     CDS             3523..3894
                     /gene="rbfA"
                     /locus_tag="I9760_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAT5044466.1"
                     /translation="MSNNFKRTDRIAEMIQRKLALIIPQEIKDPRLKGFVTISAVKVA
                     ADLGHAKIYFTVLNEDKSVVTNILNGAASYLRSALARSITLRTVPQLHFIYDESIEYG
                     QRLSRLIDEVNPPDSSSDDNN"
     gene            3881..4792
                     /gene="truB"
                     /locus_tag="I9760_002721"
     CDS             3881..4792
                     /gene="truB"
                     /locus_tag="I9760_002721"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAT5044467.1"
                     /translation="MTTIESQCSIDGILLLNKPQGMTSNAALQKTKHLFGAKKAGHTG
                     SLDPLATGMLPLCFGEATKICQYLLNADKSYETIGRLGIKTNTADCTGEVIFCIENYT
                     VSHEEMIATLEKYKGKIKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLNL
                     EQFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDE
                     LQDMPLSQRLACLIPIDQAVQHLTPVILSDSEVTAIRQGKVISNKTGAVEGEDLRLYG
                     EQSQFIGIGQALIHGDIKAKRLVSFAL"
     gene            4940..>5204
                     /gene="rpsO"
                     /locus_tag="I9760_002722"
     CDS             4940..>5204
                     /gene="rpsO"
                     /locus_tag="I9760_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAT5044468.1"
                     /translation="MSLNSAEKAEIINEYKRGDKDTGSPEVQVSLITSRIKYLTDHFK
                     ENKKDFHSRRGLQELVNKRRKLLKYLKRNDQDRYQTLIQNLGLR"
BASE COUNT         1720 a          854 c         1218 g         1412 t
ORIGIN      
        1 gtgaaaatat aatagatatc aaagctgcag cgcgcgatcc gggaaataga gccaaaattg
       61 ctgttaaaac caatgatggg cgtattgacc ccatcggcgc ttgcgttggt atgcgtggag
      121 caagggtaca agcggtttcc agcgaattag gcggtgagcg agttgatatc attctatggg
      181 atgataaccc tgcccaatta gtcataaatg cgatggctcc agctgatatt acatctatcg
      241 ttgtagatga agatacccat actatggatt tagctgttgc gaaagaacaa ttatcgcagg
      301 caattggtcg aaacggacaa aatgttcgtt tggctagcca attaactggt tggacattga
      361 atgtcatgac aacagaagag tttgaaagca agaatcaaga ggaatcaagt aaaatagtta
      421 atctgtttac cagcgcgcta gaaatcgatg aggaaatagc gacattattg gtagctcatg
      481 ggttttcttc tctcgaagaa gtggcttatg ttccgaagga ggagctgctg gctattgaag
      541 aatttgatga agagatagtt gaagaattaa gaaacagagc aaatgacaag ttattggaga
      601 tggcactgtc atcaggcaaa ggattatctg gtactccaga tgattccctt atcaatatgg
      661 aaggcatgac agaggatttg gcaaataaat tagcaagtaa aggaattaca acaatggaag
      721 atcttgcaga gcaatctgta gatgaattaa tggaaataga aggcattagt gaagaaaaag
      781 caggtgcttt aattatgaaa gcaagagaac catggtttca ataaactatg gttatcgaaa
      841 ttgaacaata cgatgctaga tgcattttaa atatattcag ctttataaaa agttgctgag
      901 aggggattaa atatggcgga tgtgacggtt aaacaattag ctcaagtcgt tggtatcccg
      961 gttgagcgct tattgaacca gttacaggaa gcagggttat cttttactga tgatcaacag
     1021 actgtgaatg aagaacaaaa gaggatcttg cttaatcatt taaaaggtag ctctaatcgt
     1081 gatattagtg ctgcgcctga aagaataact ttaagacgta aaagcatgtc tcaagtcact
     1141 gttgggcatg atatgcatag tggcaaaaca gttaatattg aggttagaaa gaaaaaaact
     1201 ttcatcaaga gaagtgcaat tcccgaacaa gcagaggtag aagagcccgt agttccgcct
     1261 gttgttgaag aacccgtcca tgaagaaatc actgtggtta gtgaagtgtc tgcagaaact
     1321 cccgaattga aagagaccga ggagcatccg gttattgaac ctgtagcaga gctcgatgaa
     1381 acagtaaaag aagaagaaaa aatcaattca gaggaaaaca cagcagagag tcaggatgag
     1441 ttaacccatg caaacactga tgtaatagag aatcttgtgg atgttgtcga ggagaccatc
     1501 cctgtttcca agaaggaaga agttaaacca gagaaagtaa gtaaaaagaa acatctggag
     1561 caaactgatt ctgatatttc tgaatttaaa aagggtaaga aaaagcctaa atatcatact
     1621 tttgaacatg atgaagagga acaagaactt catcgtcgtg gtggtcgaag taaatttaaa
     1681 aagaaaaaag gaactgaaaa atcggataaa tacagagaag ctgaagaaac attaacgcat
     1741 ggttttgcgt tacctactgc tccaatagtc agagaagtat tgataccaga gacaattaca
     1801 gttgccgaac tggcaaaacg gatgtcagta aaagccgctg aagtgattaa agttatgatg
     1861 tctttaggag cgatggcaac aattaatcag gtcattgatc aagaaacgtc tgtaatcgtt
     1921 gtagaggaaa tgggacataa accgataatt atcaaagagg atgctgttga aaccggttta
     1981 ggcgaagcaa tttccaaagg cacaaaaaca gaaggaagag ccccggttgt tacgattatg
     2041 ggacatgttg accatggtaa aacctcctta ctggattaca ttcgtcgaac caaggttgca
     2101 gcaggtgaag caggaggcat tacgcaacac ataggtgctt accatgtcag tacgccaaaa
     2161 ggtaacatta cttttttaga tacgcctgga catgccgcat ttaccgcgat gagagctcgt
     2221 ggtgcccaag caactgatat agtcattctg atcgtggcag ctgatgatgg agttaagccc
     2281 cagacaattg aagcgataca gcatgccaag gccgctaagg taccaattat tgttgcaatt
     2341 aataaaatgg ataaaccgga cgctgatcct gagcgagtta tgaatgaact atccgttcag
     2401 gaagttattc ctgaggcatg gggtggagat accatgtttg ttaatatttc ggccaaatcg
     2461 ggtatgggta tagatgattt gctggatgca attttattgc aatcagaagt attagagtta
     2521 aaagcagtga ctgatggcgc tgcaaaagga gttgtcattg aatcacgtct ggataaaggg
     2581 cgaggtcccg ttgcaacggt tttagttcaa agcggaacct tgcataaagg cgatatttta
     2641 cttgctggct ttcagtatgg gcgtgtgcgt gctttggtta gcgataatgg agatttggtt
     2701 gatagcgctg gcccatccat tccagtcgaa gtacttgggt tatcggctat tcctcatgca
     2761 ggagatgaag cggtcgttgt ccccgatgag aaaaaggcca gagaagttgc tttgtttagg
     2821 cagggtagat tccgtgatgt gaaattggcc agacgtcaaa aaaccacaat agaaggtatt
     2881 atggaaaata tgacggctac cgaatccaaa gtcttgaaca ttgtcttgaa agcggacgtt
     2941 caaggatcac tggaagcaat ttctgatgca ttgacaaaat tatcaacaga tgaagtcaag
     3001 gttgatgtga tatctagtgg tgtaggtgga ataacagagt ctgatgtcca tttggctatc
     3061 gcttccaatg ccattttaat cggatttaat gtaagagcgg atggcacagc aaagcgtctt
     3121 gctgaacaag aatctgtatc gattcattac tatagtgtta tttatgatat agtcgaccag
     3181 atcaagggtg ctttgacagg catgcttgcg ccacaattta aagaagaaat tgttggtatt
     3241 gctgaagttc gagacgtatt caagtcgccg aaaattggtg ctattgctgg atgcatggtc
     3301 attgaaggcg ttgtcaaacg taataatcca atacgtgtat tgcgttcaaa tgtagtgatt
     3361 tatgaaggta ctttagagtc attaaggcga tttaaagatg acgtattaga agtaagacaa
     3421 ggctttgaat gcggtatcgg cgtaaaaaac tacaacgatg tgaagcctgg ggatttaatc
     3481 gaggtttttg agacagtcga aattaagcgt gacttataga aaatgagtaa taattttaag
     3541 cgaactgaca gaattgctga gatgattcag cgcaaattag ctttgatcat tccccaggaa
     3601 ataaaagatc ctcgcttgaa gggttttgtt accatttctg cagttaaagt ggctgcggat
     3661 ttaggtcacg ccaaaattta ttttactgtt cttaatgaag ataaatcggt ggtgaccaat
     3721 atattaaatg gtgcagccag ttatttaagg agcgctctgg caagaagtat tactctacgc
     3781 actgtgccgc aacttcattt tatttatgat gaatcgattg agtatggaca gcgattaagc
     3841 cgattaattg atgaagtaaa tccccctgat tcaagtagtg atgacaacaa ttgaatctca
     3901 gtgttccata gatggaatcc tgctattaaa taaaccacaa ggaatgacct ctaacgctgc
     3961 tcttcagaaa accaaacatt tgtttggtgc taaaaaagct ggtcacacag gcagcttgga
     4021 tcctttagca accggcatgc tacctctatg cttcggagaa gcgactaaaa tatgccagta
     4081 tctccttaat gcggataaaa gctatgaaac aattgggagg ctaggcatta agacaaatac
     4141 tgctgattgt actggcgaag tgatattttg cattgaaaat tatactgtta gccatgagga
     4201 aatgattgca accctagaaa aatataaggg taaaatcaag caaattccat ccatgttttc
     4261 tgctttaaag cacaagggga cccctttgta tcgacttgcc agagaaggta tagagataga
     4321 acgtaaggcc agggatatag taatcagcca attgaatttg gaacaatttg atggtgaatg
     4381 ttttacatta acggtatcat gcagcaaagg aacctatata cgtaatttag tggaagatat
     4441 tggtgatacg ttgaaggcag gagctcatat gacaaaattg cataggctct acactgcagg
     4501 gtttgaaaat aatcgaatgt acactctcga tgagttacag gatatgcctt tatcacaaag
     4561 acttgcttgc ttaatcccta ttgatcaggc cgttcaacat ttaacaccag taattctttc
     4621 agattctgag gtaacagcaa ttcgccaggg caaggtgatt tcaaataaaa ctggtgctgt
     4681 tgaaggagaa gatttgcgcc tttatggcga gcaatcccaa tttattggca ttggccaagc
     4741 tttaattcat ggtgatatta aagcaaaaag gttagtttct tttgcgttgt aattacaatt
     4801 tttgtaagaa ttgctgccta ctttgtaata agtatgaaat tttttacaca aatttatatc
     4861 cactacttgt ttcatttgac atgccttggt agaatgttcg cctttaaaag aatacttagc
     4921 tgagttccag gagtaaataa tgtcgctaaa tagcgcggaa aaagcagaaa ttattaatga
     4981 atacaaacgc ggtgataaag atactggttc acctgaagtg caagtttctt taattaccag
     5041 tcgtattaaa tatttaactg atcattttaa agaaaataaa aaagatttcc attcgagacg
     5101 tggcttgcaa gagttggtta acaagcgccg caagttacta aaatatttga agcgtaacga
     5161 tcaagatcgc tatcaaacat taatccaaaa tctgggattg agag
//
LOCUS       DACTJE010000195         5198 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.197, whole genome shotgun sequence.
ACCESSION   DACTJE010000195 DACTJE010000000
VERSION     DACTJE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.197"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(6..1616)
                     /locus_tag="I9760_002723"
     CDS             complement(6..1616)
                     /locus_tag="I9760_002723"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HAT5044469.1"
                     /translation="MNAPLTVLMAQINPTVGAIDANTNKILDVIKNHQASHDVIIFPE
                     LTLSGYPAEDLLFRKEYHDKILINLHDIQDTTKDCYVIVGHPMMHIGDCYNGFSIFYQ
                     GEKIREYHKQKLPNYGVFDEARYFTPGKKDPCVLSIKNHKLGFCVCEDLWQKGPVDDL
                     LDSGISILISLNASPFDYRKYQLREELLRSYAKQGVSIIYVNQIGGQDDLLFDGQSLA
                     MDNQGIIRALAPAFEESLCTVNIDGNKIDGSITPYLDKEALIYKALVCGTRDYVRKNN
                     FPGVLLGLSGGIDSALTLAIAVDALGAEQVHAVLMPSRYTASISNEDALEQLSNLKVA
                     HSIIPIEPMFQSMIESLHPVFKDLPPDTTEENIQARIRGMLLMALSNKTGKMVLTTSN
                     KSETAVGYATLYGDMAGGFAVLKDVLKTQVYELVRYRNKISLVIPERVITRPPSAELK
                     PNQTDQDSLPEYGELDAIIMAYMEQNLSPSEIIAKGFKPQVVNKIIQLIKRNEYKRRQ
                     AAPGIKISNLAFGKDWRYPITNGFNQCI"
     gene            1900..5166
                     /locus_tag="I9760_002724"
     CDS             1900..5166
                     /locus_tag="I9760_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAT5044470.1"
                     /translation="MLNEAISKMPEAFEAKILLRGEEYFQKGQVLNIRLSDGLLKGRV
                     KGSASQIYDIHMDLKSWPAKPSRCSCAYQYNCKHAAACLFALRDREKINLQSLPSNKL
                     DRKLDVWLKNLRAQEASEIKREEATHHLIYLIDLKLRGYEHKVSIKLALAKLLKRGGY
                     GKKIIFNTLANSKKQHFMGDDNDIVAQLLFKCNVSGWFDNLLIRNTELLDKIIATGRA
                     FFIENEDTPIKKGETLTGECQWILSWNGNQILKLRHDNQNIEPLLLDESWYFDAKNAV
                     MGHLNSPYPIKQLRHLLDAPPIPLEQAELLAKKMSKNCPEFPMPNVFNQKETKQLKPI
                     PVLILDAVTEQEEHRWIDDGEENNSLYIAKVFFDYEGLLVPFAEECEKVVSLKGDVLV
                     EYQRDKEFEDLKIQELRNSIDLRPYHQWEKYHWNLPDHSFFILKDVQIVSDLESLYQQ
                     SLPYLKDHGWRIEFASPFYQEIVSADEVEWYSDIQESTTDFFSYQLGILVEGKPVSIV
                     PLVADLIQRYKGNDLDNLPDEQLVKLPLLDGRLLQLPMGRIKPLVRLLLQFGLRQIDQ
                     NQQLQINKYQLVLMQEVELAIAATKSRWQGAESLREELRKLVRLNDLPEIKIPSGLQA
                     DLRDYQHYGLNWLQFLRVSRFNGVLADDMGLGKTVQTLAHLQYEKEQSRLHKASLIIA
                     PTSLVGNWFAEAKRFTPEIRVLIYHGSDRHQDNFDDYDLIISTYGLIHRDKEKFVGYP
                     FYYLILDEAQFIKNARTKTTQIIQQLKASHRLCLTGTPLENNLGELWSLFHFLMPGLL
                     GDAKQFRLWFRTPIEKYADMGRRDLLAKRVQPFLLRRTKNQVANELPPKTEITRTIEL
                     VGAQRDLYEAIRMSMEKKVRDAIARQGLGKSHILLLDALLKLRQVCCDPRLLSMSEAE
                     IAHGTSCKLDALMELLDNLVEEGRRVLVFSQFTSMLKLIEELLIVRQYDYLKLTGQTQ
                     NRQALVDQFQQGDTPIFLISLKAGGTGLNLTRADTVIHYDPWWNPSVEDQATDRTHRI
                     GQESPVFVYKLITSGTVEEAILGMQEKKRQLVEGILSTDASRSMVLSEEDLNQFFMPL
                     "
BASE COUNT         1600 a          902 c         1108 g         1588 t
ORIGIN      
        1 tgcattcaaa tgcattgatt aaaaccatta gtgataggat atctccagtc tttaccaaag
       61 gccagattac ttatctttat tcctggtgca gcctgacgtc tcttgtattc attacgttta
      121 atcaattgaa tgattttgtt gactacttga ggtttaaatc ctttggcaat gatttcagag
      181 ggacttaaat tctgttccat gtaagccatg ataatggcat ccagttcacc gtattcgggt
      241 aaactatcct gatctgtctg gtttggtttt aattcagcag aagggggtct ggtaatgact
      301 cgctctggga taaccaatga aatcttgttt ctatacctca ctaattcata cacttgagtt
      361 ttcagtacat cttttaatac ggcaaatccg ccggccatgt cgccatataa agtggcataa
      421 cctacagctg tttcactctt gttactggta gtaagaacca ttttccctgt tttatttgac
      481 aaagccatga gcagcatccc acgtattctt gcctgaatat tttcttccgt ggtatccggt
      541 ggcaagtctt taaaaacagg atgaagcgat tcaatcatag attgaaacat gggttcaatc
      601 ggtataatgg aatgtgcaac tttaaggttg cttaattgtt ccagtgcatc ttcattgctg
      661 attgacgcgg tatatcgtga gggcatcaga acagcatgga cttgctctgc tccgagcgca
      721 tcgacagcta ttgctaacgt taaggcagag tcaatgcctc cggagagccc gagcagtacg
      781 ccggggaaat tgttttttct gacgtaatct ctggtaccac aaaccagtgc cttataaata
      841 agtgcttctt tgtcaagata aggggtgatc gatccgtcta ttttgttacc gtctatattg
      901 acagtacata agctttcttc aaaggcggga gccagggcac ggataatacc ttgattatcc
      961 atggctaaag attgcccatc aaaaagcaaa tcgtcctgac cacctatttg attgacataa
     1021 ataatggaca ccccctgttt agcataagat cttaaaagct cttctctcag ctggtattta
     1081 cgataatcaa agggggaggc gtttaaactg attaaaatgg aaatgccact gtcaagtaaa
     1141 tcgtctacag gacctttttg ccataaatct tcacatacgc aaaaccctaa tttatgattt
     1201 ttaattgata aaacacaagg atctttttta ccgggagtaa aatatcttgc ttcatcaaaa
     1261 actccataat ttggcaattt ttgcttgtga tattctctga ttttttctcc ttgataaaag
     1321 atgctgaagc cattgtagca atcaccaatg tgcatcattg gatgtccgac aatgacataa
     1381 caatccttgg tagtatcctg aatatcatgg aggttgatca gtattttatc atgatattct
     1441 ttacgaaaga gtaaatcttc cgccggataa ccggatagag ttaattctgg aaaaataatc
     1501 acatcatggc ttgcctgatg gtttttgatg acatcaagaa ttttatttgt attcgcgtca
     1561 atagctccaa ctgttggatt aatttgagcc atcagtactg tcaagggtgc attcatgaaa
     1621 atctgtcaat taattcgtat ataggaattg tagcttagag ggcattgata tggctacaga
     1681 tgaagaggat ggtagtcagg ttaattggag aagtttttat ttttgcattt atccgctccc
     1741 ttatggtcgc ggctcggatt atggttcact ctgagtcaga taggatcatg gttaaaaccg
     1801 agccgcaacc gtaagggagc ggatagaaca acccattcta ttgtcaaaaa gcgtaaaaaa
     1861 tgactacaat atacgatttt tcctggacaa atcctaggta tgttaaacga agcaatatca
     1921 aaaatgccag aggcttttga agctaaaata ttgcttcggg gtgaagagta ctttcaaaaa
     1981 ggccaggtat tgaatattcg cttaagtgac ggcttgctca aagggagagt gaagggtagt
     2041 gcaagtcaaa tttatgatat ccatatggat ttaaaatcat ggccagcaaa accttctcga
     2101 tgttcatgtg cttatcaata taattgcaaa cacgccgcag cttgtctttt tgcattaaga
     2161 gacagagaaa aaataaacct gcagtcactg ccttctaaca aactagatcg aaaattagat
     2221 gtttggttaa aaaatttacg agcacaagaa gcatcagaaa taaaaaggga ggaggcgaca
     2281 caccatctga tttatttgat tgatttaaag cttcggggtt atgagcacaa agtgagcatt
     2341 aaattagctt tggccaaact attaaaacgc ggcggttacg gaaaaaaaat aatctttaat
     2401 actctggcta attcaaaaaa acaacatttt atgggtgatg ataatgacat cgtggctcaa
     2461 ttgttattta aatgtaatgt atccggttgg tttgataatc ttttgattcg gaatacagag
     2521 cttcttgaca agatcattgc aaccggaaga gcttttttta ttgaaaatga ggatacccct
     2581 ataaaaaagg gagagacttt aactggtgaa tgccagtgga ttttatcctg gaatggcaat
     2641 cagattttaa aattaaggca tgataatcag aatattgagc cattattatt ggatgaaagt
     2701 tggtattttg atgctaaaaa tgcggtaatg ggtcacttaa actctcctta tcccatcaag
     2761 cagcttcgtc atttgttgga tgcaccacct attcctcttg aacaagccga gttattggct
     2821 aaaaaaatgt ctaaaaattg ccctgaattt cccatgccaa atgtgttcaa tcaaaaggaa
     2881 acgaaacaat taaaaccaat tcctgtgtta attctcgatg cggttaccga acaggaagaa
     2941 cacaggtgga ttgatgatgg ggaggaaaat aattctcttt atatagcaaa agtctttttt
     3001 gattatgaag gattgcttgt tccttttgcg gaagagtgtg aaaaagtggt gtctttaaaa
     3061 ggagatgtat tagttgaata ccaacgtgat aaggaatttg aagatttaaa aattcaagag
     3121 ttgagaaatt caattgattt gaggccatat catcaatggg aaaaatacca ttggaattta
     3181 cccgatcact ctttttttat tttaaaagat gtgcaaattg tttcagactt ggaaagtctt
     3241 tatcagcaat cgttacctta tttaaaagac catggatggc gtatagagtt tgcaagcccg
     3301 ttttatcaag agattgtttc tgctgatgaa gtggaatggt attccgatat tcaggaaagt
     3361 acgactgatt ttttttccta tcaattgggt attttggttg aaggcaagcc agtgagtatt
     3421 gtgccgctgg ttgcggattt gattcaacgt tacaaaggga atgatttaga taatttacct
     3481 gatgagcagc tggttaaact cccattgttg gatggtcgtt tattgcaatt gcccatgggg
     3541 cgaattaagc cattggtccg tttgttatta cagtttggct tgcgccaaat cgatcagaat
     3601 cagcaattac agattaataa atatcaactg gtgttaatgc aggaagttga attggctatt
     3661 gctgcaacca aatcacgttg gcaaggtgct gaatcattaa gagaagaatt aaggaagctg
     3721 gtcagattaa atgatctgcc agaaataaag attccgtctg gtcttcaagc ggatttgagg
     3781 gattatcaac actatgggtt aaattggtta caatttttgc gggtcagtcg ttttaatggt
     3841 gtattagcgg atgatatggg acttggcaaa acagtgcaga ctctcgccca tttacaatat
     3901 gagaaagaac aatcaagatt acataaagcc agtttaatca tagctcccac cagtcttgtt
     3961 ggtaattggt ttgccgaagc caagcgattt acgccagaga tcagagtatt gatttaccat
     4021 ggaagtgata ggcatcagga caattttgat gattatgatt taattatttc tacctacgga
     4081 ttaattcaca gggacaaaga aaaatttgtt ggttacccct tttattatct cattttggac
     4141 gaagcgcaat tcattaagaa tgcacggaca aaaacaacac aaatcattca acaattaaag
     4201 gcttcacacc gtttatgtct tactggaact ccactagaga ataatttagg cgaactctgg
     4261 tctttatttc attttctaat gcccgggcta ttgggtgatg caaagcaatt caggctctgg
     4321 ttcagaacgc ctatagaaaa atatgccgat atgggaagaa gggatttgtt agcaaaaaga
     4381 gtgcaacctt tcttactgcg ccgaaccaaa aatcaggtag ccaatgaact cccgcccaaa
     4441 acggaaataa ctcgcactat agagttggtt ggtgctcagc gggatcttta tgaggctatc
     4501 cgtatgagca tggagaaaaa ggtgagagat gccatagcaa ggcagggatt ggggaaaagt
     4561 catatattat tacttgatgc cttgttgaaa ttgcggcagg tttgttgtga tccaagatta
     4621 ttgtctatgt ctgaagccga gattgcccac ggcacttcat gcaagctcga tgcattgatg
     4681 gagctgcttg ataatttggt tgaagaggga aggcgagtac tggtattttc acaatttaca
     4741 tcgatgttaa aactgattga ggaattatta atagtcaggc aatacgatta tttgaaattg
     4801 acgggacaga cccaaaacag acaggcatta gtcgatcaat ttcaacaagg tgatacgcca
     4861 atatttttaa ttagtttgaa agcaggaggc acaggactta atctgactcg tgcagatact
     4921 gtgattcatt atgatccctg gtggaatcct tctgttgagg accaggctac tgacagaact
     4981 catcgaatag gccaggaaag ccctgttttt gtttacaagt tgattacatc aggtactgta
     5041 gaagaggcaa ttcttggtat gcaagagaag aaaaggcaat tagtggaagg gattttgtct
     5101 acagacgctt ccaggtcaat ggttttaagt gaagaagatt tgaatcaatt ttttatgcct
     5161 ttatgaataa aagtaacggg ttattatccg ctccctga
//
LOCUS       DACTJE010000196         5132 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.198, whole genome shotgun sequence.
ACCESSION   DACTJE010000196 DACTJE010000000
VERSION     DACTJE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.198"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1493)
                     /gene="ankF"
                     /locus_tag="I9760_002725"
     CDS             complement(<1..1493)
                     /gene="ankF"
                     /locus_tag="I9760_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkF/LegA14/Ceg31"
                     /protein_id="HAT5044471.1"
                     /translation="MKDQLAKIFEKYPFVHLDKSGELSIIVPLTGAASISDNFTADET
                     KPIFLYLFGDNTCKSDLERRTFFYGKQKSGGRHSVKPFTELFSNLIHELNGLKSSTEI
                     RVDEQLFLETIIANLNELKEQALAFRKGNPGFEDMRNDFSHSDEIMVSKSEFVVTVYP
                     DRVRQFDLPNVMSLINPGESRYKQLLAQSDINQCLEALSLVQKPAAEGNLDLIADALG
                     KLDADPIDYLCKKDNKDPQALFKAVYEILQKQDTSLENQQSAVFEVMEGNLVAAFGDN
                     EDFSTWQKEEIVDAIKSIFKQANLLSEQSGTKQKTFADVYNQAFDKYNESTNDDSESG
                     CKRRNTYQLFILQTFLYLCSLQLRLKNKEKAKKFLGLFEDRGTLNNLVLQLCNDEKAF
                     MRMMADEFGLTSAEYASIAESTYGIVLAHIDAEHFDELRIACSAQLLQDSNYIVMGGR
                     LCWSTSSINEEGNIRSLEQQKKISSENFEIFYNQRQSYIDKSREFQQ"
     gene            1717..2211
                     /locus_tag="I9760_002726"
     CDS             1717..2211
                     /locus_tag="I9760_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044472.1"
                     /translation="MAFQRSFFSHIEDDWRYYQNIRAKYSDAIPIPQRKYFEPIHSID
                     SFATLAVRPIEKPLWLGVHTAGFLLKAIIHLVGALVLSPFALIFAVCVPRSELREQTV
                     SSFKSTAAGSIVAAGMACVALLSTLMSLIFNPLYLLSRSAATGIDHLNSVTESCCGLT
                     IAKI"
     gene            complement(2259..2708)
                     /locus_tag="I9760_002727"
     CDS             complement(2259..2708)
                     /locus_tag="I9760_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044473.1"
                     /translation="MSFNNFLKTFNEFLLEQCGTTYQVADHYLKGKDKPLKSVFFAPY
                     SSAPNFFYRAGHVITAPISFSIITLELVSSSLYLSLKSLNSLVFSDKKAAKIHIIDSV
                     VHFAVSLITAIGVIVSPIINLIDLIGGAISTMKVKSEPAEQMRPSVL"
     gene            3013..3459
                     /locus_tag="I9760_002728"
     CDS             3013..3459
                     /locus_tag="I9760_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5044474.1"
                     /translation="MLNFKWCEFSELTSKQLYDILALRASVFVVEQHCPYLDPDGRDF
                     FSMHLLGLEENSLVAYLRLFLPTEIENHLTFGRVVTAKSARTKGYGRKLIRELLSYCD
                     MHYPGIRIQCSAQNYLKKFYEEFDFKSYGSVYEEDGIPHITMQRTL"
     gene            3769..4398
                     /locus_tag="I9760_002729"
     CDS             3769..4398
                     /locus_tag="I9760_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044475.1"
                     /translation="MTKIVYLHNDPDKMLYYKDSVPKDLKVVEPDAKMMLEILEQKLK
                     LLEVVDLDWFYCAICMELPKEPVFYPTSEGISNLYEKSELVKWFSRDPRNKSDPVGLG
                     LNVTISQYKTPTPKELLDFVIKSTGFAEEVFIDHYKKPLAKKISPEEELLLEKPDNTR
                     RNEIDSPTHSQSRSCWSLFLYSIFGGKDNRKVMQEKTDSLTSYDIQPHH"
     gene            4604..>5132
                     /gene="ankD"
                     /locus_tag="I9760_002730"
     CDS             4604..>5132
                     /gene="ankD"
                     /locus_tag="I9760_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkD/LegA15"
                     /protein_id="HAT5044476.1"
                     /translation="MLTPPPDSKISTTDKSLDKLSAPLDMLKQMNESTMEQTKLDELR
                     KKMSLQAEILNKAKADNDMFFRLLIELMSLKLQGELFKEQLSKISKESGYDSAQSALI
                     QATNSEGQSPLQYALQKQDFSTAKYFLDNGAKAGPIEKAVFEIALDSKAAKEFGFPPL
                     PPEKEKLHPVKNFGLV"
BASE COUNT         1646 a          985 c          884 g         1617 t
ORIGIN      
        1 atctgttgaa actctcttga tttatcaata taagattgtc tttgattata aaaaatttca
       61 aaattttcag atgaaatttt cttttgctgc tccaaacttc gtatatttcc ctcttcatta
      121 atagaactgg tgctccaaca aagacgccct cccattacga tataattgga atcctggagc
      181 aattgagcac tgcatgctat tcttagttca tcaaaatgtt cagcatcaat atgagccaag
      241 acgatgccat aagttgattc agcaatagac gcgtattctg cagaagtcag cccaaattca
      301 tcggccatca ttctcataaa cgctttttca tcgttacata attgtaatac caaattattc
      361 aacgtacccc tgtcttcaaa caaacccagg aatttttttg ccttttcttt gttttttaaa
      421 cgcaactgca aagaacacaa atagagaaac gtttgtaaaa taaacagctg ataagtgttc
      481 ctgcgtttac atcctgattc agaatcatca ttagtgcttt cgttatattt atcaaaagcc
      541 tgattgtaga catctgcaaa tgttttttgc ttggttcctg attgttcact cagcaaattt
      601 gcttgtttaa atattgattt aatggcatcg actatctctt ccttttgcca ggtactaaag
      661 tcttcgttgt ctccaaaggc agcgaccaaa ttgccttcca tgacttcgaa aacagctgat
      721 tgttgatttt ctaaagaagt atcctgtttt tgcagaattt cataaaccgc tttaaataaa
      781 gcttgaggat ctttattatc ttttttgcat aagtaatcga tggggtctgc atccagtttt
      841 cccagcgcat cagcaatcag gtccaagtta ccctctgcag caggcttttg cacaagagac
      901 aatgcttcca ggcattgatt aatgtcggac tgggcaagta attgcttgta gcgggattca
      961 ccggggttta taagactcat tacgtttggc aaatcaaatt gtcgcactct gtctggatac
     1021 accgttacaa caaactcgct ctttgatacc atgatttcat cgctgtgact aaaatcattc
     1081 ctcatgtctt caaagcctgg atttcctttt ctgaaagcga gagcttgctc tttcaactca
     1141 ttgagatttg ctatgattgt ttcaagaaat agttgctcat ccacacggat ttcagtagac
     1201 gatttgagac catttaattc atgaatcaaa tttgagaaca attcggtaaa gggttttaca
     1261 gagtgacgac ctccggattt ctgcttacca taaaaaaaag ttcttctttc taaatccgat
     1321 ttgcacgtat tatcgccaaa taaataaaga aaaattggct tggtttcatc ggcagtaaaa
     1381 ttgtcggaaa tactggccgc gcctgttaaa ggcacaatta ttgatagctc acctgattta
     1441 tctaaatgaa caaaggggta tttttcgaat attttggcta attggtcttt caatttcgtt
     1501 atactctttc attttaagct tttttggcgc cattgtaata tagtttgctt aagacaaaat
     1561 taataacact gtgggcataa ataagcccag aaaaaacctc ttgttgtctc tgccaacaag
     1621 aaaatcccaa gatttgaaaa atgaataact tgcctattat ttacaaacac tgccaatttt
     1681 tttaatatcc gctgattcaa taacaaggag tttccagtgg cttttcaaag atcatttttt
     1741 tctcatatcg aagacgattg gcgttattat caaaacattc gtgcaaaata ctctgatgct
     1801 atacccatac cacaaagaaa atatttcgaa cccattcatt ccatagattc attcgccaca
     1861 ttagcagtac ggcctattga aaaacctctt tggcttggtg ttcacacagc aggttttttg
     1921 ctgaaagcga taatccactt agtaggggca ttagttcttt caccatttgc tttgatcttt
     1981 gcggtatgtg ttccgagatc cgaattgaga gagcaaactg tctcatcatt taaatctact
     2041 gctgccggat ctattgttgc tgcaggtatg gcttgtgttg cattactctc aactttaatg
     2101 tcgcttattt ttaatccgtt gtatctatta agtcgaagtg ccgccacagg aatcgatcat
     2161 cttaattcag taaccgaatc ctgttgcggt ctgactatag caaaaatctg attaggtggt
     2221 tcaatcgcaa cccttattta tttcaaatct ggcgaaaact agagaacaga tggtctcatc
     2281 tgttctgctg gctcactttt taccttcata gttgatattg ctcctccaat caaatcaatc
     2341 aaattaataa tggggctaac aatcacacca attgcggtaa taagagaaac agcgaaatgc
     2401 accacgctat caataatatg aatctttgcg gcttttttat cagaaaaaac caggctgttg
     2461 agagacttta agctcaaata gagagacgat gaaacaagct ctaaagttat aatagaaaaa
     2521 ctaatcggtg ccgttatcac atggcctgct ctgtaaaaaa aattaggggc agatgaataa
     2581 ggagcaaaaa aaactgattt gaggggtttg tctttccctt tcaaataatg gtcagcaacc
     2641 tggtaagtgg taccgcattg ttctaataaa aattcattga atgtttttaa gaaattatta
     2701 aacgacatcg atagatactc cctgttaaaa aaatcttcat aataatatat gaatttgcat
     2761 taattactaa agataatttc gataatgatt tttacttcaa tctatcgcct gtaaatttgc
     2821 cctggtataa tcagaacttg ccaaatacat ccagttggaa gcaaaaaatg tttttaataa
     2881 tgggtgttat ttaaactaat tgacttaatt aaaaccattc tttaacaaaa tcctgatcat
     2941 tactccactt gatttcagag tcataacttc agcataatgc atcgaagatt ttagccggat
     3001 tgggagtttt tcatgcttaa ttttaaatgg tgtgagtttt cagaattaac aagcaaacaa
     3061 ctctatgaca ttctggcatt acgagcgagt gtttttgtag tagaacagca ctgcccctac
     3121 cttgatccag acggtagaga ttttttttcc atgcatttat tgggcctgga agaaaattct
     3181 ctcgtggctt atttgcgcct gtttcttcca actgagatcg aaaaccatct cacatttggc
     3241 cgtgttgtta cagctaaatc agcgcggacc aaaggatatg gaagaaaact gatacgtgaa
     3301 ttattgtctt attgcgacat gcattaccct ggtattcgca ttcaatgctc tgcccaaaat
     3361 tacttgaaaa aattctatga agaattcgac tttaaaagtt atggctcggt ttatgaggag
     3421 gatggtattc cccatattac catgcaaaga accttataaa accgcaataa ccaatccggg
     3481 tttcctggaa gtaatctatc agcccgatca aattttaaat tgacattagt gctacaataa
     3541 agcacacttt ttatcatgtt tactcattaa taatattatt taacgcgagc tatggataaa
     3601 atcagtcatt atctcgaagc ctagtatcgg atttcatgtt tcatgccagg gcttcgagac
     3661 aatgcttatg catctcctca gcctgagtgt tccctgggta tcgactggtt gatatatcat
     3721 ctttatccat aactcacgtt atttaaagta ttattctatg gagaggttat gacgaaaatt
     3781 gtttatctac acaatgaccc ggataaaatg ctctattata aggacagcgt tcccaaagat
     3841 ttaaaagtgg ttgaaccaga tgccaaaatg atgttagaaa ttttagagca aaaattaaaa
     3901 ctactcgaag tagttgattt agattggttt tattgtgcaa tttgtatgga acttcctaaa
     3961 gagccagtgt tttatccaac ctcagaaggc attagtaatc tttacgaaaa aagtgaatta
     4021 gtcaaatggt tttcaagaga ccccagaaat aaatccgacc ccgttggact tggtttaaat
     4081 gttactatat ctcaatataa aactcctact ccaaaagaat tgcttgattt tgttattaaa
     4141 tcaacaggat ttgctgaaga ggtgttcatt gaccattaca aaaaaccact agcaaaaaaa
     4201 atttcccctg aggaagagct cttacttgag aaacccgata ataccagacg caatgaaata
     4261 gatagcccca ctcattcaca gtcaagatcc tgctggagtc tctttttgta tagtattttt
     4321 ggtggtaaag ataacagaaa agtaatgcag gaaaaaactg attccctaac atcgtatgat
     4381 atacagcccc atcattaatg aaaataaatc aagtttttta gacggttttt ttgttatgca
     4441 cagaacttac acccccaaag atcaaggcaa aaatgtgttt aatgagagag tttcgataaa
     4501 ctaaatgacc gaattaaaaa caatttttaa caaagagatt gggggttttg taagtcctga
     4561 tgtataataa tgatatagtt caaccaatca accaggatgt tccatgttga ctcctccgcc
     4621 tgactcaaaa atttcaacca ccgataaaag tttggataaa ttaagcgctc cattggatat
     4681 gcttaaacaa atgaatgagt caacaatgga acaaaccaaa cttgatgaac tacgaaaaaa
     4741 aatgtccctg caagcggaaa tacttaataa agcgaaagca gataatgaca tgttttttcg
     4801 tttacttatc gaattaatgt cattaaaact ccaaggtgaa ttatttaaag aacaattaag
     4861 caaaatttct aaagaaagtg gttatgacag cgcacaaagc gcattaattc aagccacaaa
     4921 ctcagaaggg caaagccctt tacaatacgc cctgcaaaaa caagacttct ctacggcaaa
     4981 gtattttctg gataacggag caaaagcggg tccgatagaa aaagccgtct tcgaaattgc
     5041 tcttgacagt aaggcggcta aagaatttgg gtttcctcca ctcccacccg aaaaggaaaa
     5101 actgcaccct gtcaaaaatt ttggtctggt tt
//
LOCUS       DACTJE010000197         5086 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.199, whole genome shotgun sequence.
ACCESSION   DACTJE010000197 DACTJE010000000
VERSION     DACTJE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.199"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..637)
                     /locus_tag="I9760_002731"
     CDS             complement(<1..637)
                     /locus_tag="I9760_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0172 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044477.1"
                     /translation="MLYNKWGYLMTTLYIKNLRYNEVAVCRALFEAAEKTEEGIKTKR
                     YGMQMTADMPGLWSPLSNYDIENDLRMARVHRNGRMREIGYLTVNLVFQFDSLDVTEY
                     ETKYGISAKDVLEKAGFTCSQDAAGYSEPASTSTRLLGQESKPEKPTQTSVVNTSFFA
                     LKNVDTDKRFTKSTKLGILYEARSYGKIEDDTLKSYGFTDGQWERQKEGIEN"
     gene            827..1393
                     /locus_tag="I9760_002732"
     CDS             827..1393
                     /locus_tag="I9760_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-box protein"
                     /protein_id="HAT5044478.1"
                     /translation="MKAKYDPTKPGLQKLPPEIKVMILEFLDAKSKLALSQTNYGWRD
                     LILDRPEYTKEITNTLFRLDKKRHRQAIAQMMSGRVTASSMAKLFEELLCFSIPSSYV
                     FLIFFASQKSVALIEVLTVILVFAAITSLAHDLVDYFIESDTKAEKQHAHRRAFQFFA
                     QPSQSAAQQNLEEENLSADPKACQCEPL"
     gene            1659..2414
                     /locus_tag="I9760_002733"
     CDS             1659..2414
                     /locus_tag="I9760_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT5044479.1"
                     /translation="MYVFRRFSKPIITFLHSLELAFRFLGHLVHSTIKVIFGKLSIVW
                     PNTLEMIYYSGARLVILLSFIGILLGATVSQTVYALLKPFQLHQRVLPIVQNILTHEI
                     LPVLIGFILCIQAALHMVNTRLEHYQENPEAIILAQVLPIIVGMNIASLLLYVYLVSA
                     IFFSFYLTFHFMLGFTNNEFLSYVVSSTTLFDLIYSVVKTLILCIIVGLTSGYYYYEA
                     SIRHIYLRKAVSRILTRGSFWLIIASMYITLTF"
     gene            2482..3168
                     /locus_tag="I9760_002734"
     CDS             2482..3168
                     /locus_tag="I9760_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HAT5044480.1"
                     /translation="MILGGTFFYIEYKRAQKQTFVMFFKGSLKGLVTTAPVTYRGVKI
                     GEVKVIEITENKEHSKVLIPVYVQFFVERTYGFSQDPIHLLIDNGYVANITKPNLLTG
                     VAEIELIKPTPAVKYKQTYYHSYPVFPTHNSAEKYTSMEEAFEAAKKAFEDVSELVRS
                     KEIQDTLEAIQKVSENLGQLASSLNQDVPSVVAYLNQSLKQITSAAYSTQNLTDYLSR
                     YPESLLRGKR"
     gene            3165..3890
                     /locus_tag="I9760_002735"
     CDS             3165..3890
                     /locus_tag="I9760_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAT5044481.1"
                     /translation="MKNNRHFLKFYCIRDLSWLSSVRASFIYSPFERITSSPFSSYIE
                     LRKKPVKFLLILLIALTLCDCGRSKETQFYLLTPIPPQKNSRNSYNHLQIGVDEVSIP
                     DYMKKPQLMIHQTAHHLTLEEFNQWAGALDKNITLVLTTNLSTLMPGVVVQTSPWNNK
                     FHPDYHLQVMISQFEIDIHGNSILRAEYLIYRKEELIYKGNAYYHIKVPLISIETLVQ
                     SMNTNLTHLTEEIARFFIKNTTK"
     gene            3991..4401
                     /locus_tag="I9760_002736"
     CDS             3991..4401
                     /locus_tag="I9760_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HAT5044482.1"
                     /translation="MIGKDKWEILTEKMTNLHIYETDLSEKFILGSGKGGQKLHKTAS
                     TVYLKHLPTGMEVKCQESRSREDNRYFARQRLCEKLQAVFSDEKTKTQQRIEKIKRQK
                     KRRSRRSKQKMLDEKSRQSQLKTLRKSPRSHDIE"
     gene            4676..>5086
                     /locus_tag="I9760_002737"
     CDS             4676..>5086
                     /locus_tag="I9760_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAT5044483.1"
                     /translation="MAGTSLLTLIDDIATALDDIAAMTKVATKKTAGVLGDDLALNAE
                     QIIGIHANRELPVIGAVAKGSAVNKLILIPLAILINFFVPALVGILLILGGLYLCFEG
                     FEKIVEKIFHKKNEPAKKKTDTQTIDMRQFEKEKI"
BASE COUNT         1611 a          978 c          869 g         1628 t
ORIGIN      
        1 tattctcgat gccttctttc tgcctttccc attgtccgtc ggtaaaacca tagcttttca
       61 aagtatcatc ttctatttta ccgtaggatc ttgcctcgta taatattcct agttttgtcg
      121 acttggtaaa tcttttatct gtatccacat tctttagagc aaaaaatgag gtattaacaa
      181 cacttgtttg ggttggtttt tcgggtttgc tttcttggcc taataacctt gttgaagttg
      241 aagcaggctc agaataaccc gcagcatctt gactgcatgt aaagccggct ttttctaaaa
      301 cgtcttttgc tgaaatgcca tattttgttt catactctgt aacatcgagg ctatcaaatt
      361 gaaatacaag attaactgtc aaatatccaa tttccctcat tcttccattg cgatgaactc
      421 ttgccattct gagatcattc tcaatgtcat aattagaaag aggagaccat aaaccaggca
      481 tatcagccgt catttgcatg ccatatctct tggtctttat tccttcttca gtcttttcag
      541 ccgcttcaaa taaggcacga cagactgcta cttcattata tcttaaattt tttatgtaaa
      601 gcgttgtcat tagataaccc catttattgt aaagcaggca ttttatccat ttttgctcaa
      661 aataaaagcg tttttaccga ttggacttaa tgaaaacgca ttacttactc ccgcatatca
      721 atattttata tagctcctat acaaataaat catttttgac acaatgcaaa accacctaaa
      781 ataaatttta attttttcaa aaattaaata atttaggtta ttcactatga aagcaaaata
      841 cgaccccaca aagcctggac tccaaaagtt acctcctgaa atcaaggtaa tgattcttga
      901 gtttcttgat gccaaatcaa aactagctct ttcacagaca aattatggtt ggcgtgattt
      961 aattctagac cggccagaat ataccaaaga aataacgaat acattatttc gtcttgataa
     1021 aaaacgccat cgtcaagcaa tagcacaaat gatgtcagga agagttacag caagttctat
     1081 ggctaagcta tttgaagaat tactatgttt tagcatacct tcgtcctatg tgtttttaat
     1141 ctttttcgca tcgcaaaaat ctgtggcgct tatagaagtc ttaaccgtaa tccttgtgtt
     1201 tgctgcaata acctctctcg cccatgatct ggtggattat tttattgaaa gtgatacaaa
     1261 agctgagaaa cagcatgcac atcgccgtgc ttttcaattc tttgcccaac ccagtcaaag
     1321 cgctgcacaa caaaacttgg aggaagagaa tttaagtgct gatcccaagg cctgccaatg
     1381 tgagccattg tagttggggt cttggcccga tatttaccga attcctctac ttaattcttc
     1441 gatgtttatt atatttacaa ccttattttg atcctccact cacccgaata tcactagcaa
     1501 caaaccaaac agaatgttat tcttagctgt agtaaaagag caaaaagtct cactgctatt
     1561 gagttaagaa ttaactcctt tttgcctgat gcaagcatct tgctaacgaa gctattattc
     1621 ttttcgcggc attttgaaag aggactaatg gattagccat gtatgttttt cgccgttttt
     1681 cgaagcctat tattactttt ctccattcgc ttgagctcgc ttttcgattt ttaggccatt
     1741 tggttcatag cactatcaaa gttatttttg gcaaactgtc catcgtctgg cctaacacac
     1801 tagaaatgat ttactattct ggagcgcggc ttgttattct actgagtttt attggtatat
     1861 tattaggcgc tactgtttca caaacggttt atgcgctctt aaaacctttt cagctacatc
     1921 aacgagtact tcccattgtg caaaatattt taacccatga aatattaccg gtacttattg
     1981 gcttcatttt atgtatccaa gccgccttgc atatggttaa cacccgtttg gagcattacc
     2041 aggagaatcc agaggcaatt atccttgctc aagtattacc aatcattgta ggtatgaaca
     2101 ttgcctcctt actgctatac gtttatcttg tatccgctat cttttttagt ttttatctca
     2161 cttttcattt tatgttaggg tttacaaaca atgaattttt atcctatgtt gtcagctcga
     2221 caaccttatt tgatctgata tactcggtcg ttaagacatt gattttatgc atcatagttg
     2281 ggttgacttc tggttattat tattatgaag catctattag acatatttat ttgagaaaag
     2341 cagtttctcg aattttaacg cgtggctcat tctggctgat cattgccagt atgtatatta
     2401 ctcttacgtt ttagggacga cgtgcataac gaacgattct atacattagt gggtattttt
     2461 atagtaggcg ccctatgttt aatgattcta ggcggtactt ttttttatat agaatacaaa
     2521 cgcgcacaaa aacaaacttt tgtcatgttt ttcaaaggct ctttaaaagg gctagtcact
     2581 actgccccag taacctatcg aggcgtgaag attggtgaag tcaaagtcat agaaatcact
     2641 gaaaataaag agcacagtaa agtacttatt cctgtttacg tacaattttt tgtcgagaga
     2701 acctatggtt ttagccaaga ccctattcac ctgcttattg ataatgggta tgtagcaaat
     2761 atcaccaaac ctaacctgtt aacaggcgtt gctgaaattg agttgattaa gccaacccca
     2821 gcggtaaaat ataaacaaac atactaccat tcttatcctg ttttcccgac tcacaattca
     2881 gctgaaaaat atacatctat ggaagaagcg tttgaggccg cgaaaaaggc ttttgaagac
     2941 gtcagtgaat tagttcgttc caaagaaatt caagatactt tagaggcgat acaaaaagtt
     3001 tctgaaaatt tagggcaact agcctctagc ttaaaccaag atgtgccgag tgttgttgcg
     3061 tatttaaatc aaagcttaaa acaaattacc agtgcagctt actccactca aaacttgacg
     3121 gattatttgt ctcgatatcc tgaatcttta cttcgaggca aaagatgaaa aacaatagac
     3181 attttttaaa attctattgc atcagagatt tgagttggct aagtagtgtt cgagcctctt
     3241 ttatatattc cccatttgaa cgcatcacat cctcgccttt ttctagctat attgagcttc
     3301 gaaagaaacc tgtgaagttc ctgttaatct tgctcattgc cttaacactc tgcgattgcg
     3361 gtcgaagcaa agaaactcaa ttttatttat taacacctat tccccctcaa aaaaacagcc
     3421 gtaactccta taatcattta cagatcggtg ttgatgaagt gagtattcct gattatatga
     3481 aaaaacctca attaatgatt caccagacag ctcatcacct aactctcgaa gaatttaacc
     3541 aatgggctgg ggccttggat aagaatatta ctcttgtatt aactactaat ctttcaactt
     3601 taatgccagg agtagtagtt cagacctcac cttggaacaa taaattccac ccggattatc
     3661 atcttcaagt aatgatttca caatttgaaa ttgatattca tggtaacagt attttaagag
     3721 ccgaatacct tatttaccga aaagaagagt taatctacaa aggaaatgct tattaccata
     3781 ttaaagtccc tttaatcagt attgaaacgc ttgtgcagag catgaatacg aacctgactc
     3841 atttaactga agaaattgca cggtttttta taaaaaacac gactaagtaa tatactcata
     3901 gtgtcttcaa atcaagaaaa attattttga ttgaattcag ctattataat gctttaaagg
     3961 cattatttta tgggataata aagagacttc atgattggca aagataaatg ggaaatactg
     4021 actgagaaga tgaccaatct tcatatctat gaaacagatc tcagcgaaaa atttatacta
     4081 ggcagtggca aaggcggaca aaaactacat aaaacggcat caacagtcta tttgaaacat
     4141 ttgcctaccg gtatggaagt aaaatgccag gaatccagga gtcgtgaaga taaccgctat
     4201 tttgcgaggc aacggctttg tgagaaatta caagccgtat tcagcgatga aaaaacaaag
     4261 acacaacaaa gaattgaaaa gataaaacgc cagaagaaga gacgttcaag aagatccaaa
     4321 caaaaaatgc tggatgaaaa atcaagacag agccaactca agacactaag aaaaagcccc
     4381 cgatctcatg acattgaata ataggcacat taaccactta tcattttagt taattagtta
     4441 aatcaaaata tccgacaacg tccctaaagt tttttgtaga ttcctgaact ttaaccaagg
     4501 ggcattattt tcagtttgct tcgatcaatt ccttctcaca cgtctggtga ttttaaaatt
     4561 gaaattttta tatcgtcagg ttcgagtctt tgaaacggag ataagacatc tttttgcaat
     4621 gctttgaatt tgttttagta ttgaaaggaa tcattaaatt aaggtcagat ccgatatggc
     4681 aggaacaagt ttactaacat tgattgacga tattgcaacg gcactggatg acatagctgc
     4741 catgactaaa gtggcaacta aaaaaactgc cggtgtgttg ggcgatgact tggcactcaa
     4801 cgccgagcaa ataataggca tacatgcgaa cagagaatta cctgtcatag gagctgtagc
     4861 caaaggctct gctgttaata aattaatatt aattccatta gcgattttaa taaatttttt
     4921 cgtccccgca ctggtgggta tcctattgat tttgggcgga ttgtatttgt gttttgaagg
     4981 ttttgaaaaa attgtagaaa aaattttcca taaaaaaaat gagcctgcaa aaaagaaaac
     5041 agatacgcag accatcgata tgcgccaatt tgaaaaagaa aaaatt
//
LOCUS       DACTJE010000198         5033 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.200, whole genome shotgun sequence.
ACCESSION   DACTJE010000198 DACTJE010000000
VERSION     DACTJE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.200"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            49..1938
                     /locus_tag="I9760_002738"
     CDS             49..1938
                     /locus_tag="I9760_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAT5044484.1"
                     /translation="MPSTRNIETHNDSNPTLRALSLSALGIVYGDIGTSPLYTFKTVI
                     LLAGGGTPAVNTIMGSVSLIIWTLIIIASVKYIHFALRIDNDGEGGILALMSLLSLKL
                     KQRPFIIAVGLMGAALIYGDGTITPAISVLSAVEGLEILSPSLKYYVLPIAITILITL
                     FAIQSKGTATIGKAFGPVMAFWFLTIGILGARGVIQHPSVLAAINPIYGLSFLFSNGA
                     TGFFILCGVFLCVTGAEALYADLGHFGTAPIRCAWFGLAFPSLIFNYLGQAALVLEGA
                     STEHNIFYMLCPGDFLLPLIILSTVATIIASQAIITGAFSMTRQAMQLGWLPRLRVTQ
                     TSSEGYGQIYIGVVNWLLMLATLGLTIGFGSSEKLAAAYGIAVSATMLCTTLLLFIAL
                     HKLWKWDIITSGLVAGLFMIVDASFFAANLTKFINGGYIPITLAIIIYSMMYIWHKGY
                     QTIAIKQKEKNITVASFLDSIQKERVVRVPKTAVFLTSKEQDIPPILVWHVKKNHVLQ
                     DKVIILKINNLSIPRCKPGDRLQIVETGTGIWHAVANYGFMEQPHIPKLLKKLEAQGY
                     DINIKDITYYIGHETIFVRNVRHTLSKYIKILFVFMHRNALPMSNYFHLPPESVFEIG
                     RQIEI"
     gene            2148..3935
                     /locus_tag="I9760_002739"
     CDS             2148..3935
                     /locus_tag="I9760_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044485.1"
                     /translation="MIEMVQFAKPSIGPPADCGRINLLIGRELGQYQNIIQKWIANLE
                     DNAKQRPSNISEFKFGKTLGYLLLQSLKKANLHPDNFRSFGFDPWEEPHYQSALIAGK
                     YVITQDTYRIYFDIPKIDHSDEEKIKANQDHAQILYFTTMTNDGLMVFTFEDKVENIN
                     SRLFVELAKNIKINSEAWSQLIQFTEKNLLYEQDDISSKLPQVFGLILYASISPEHRE
                     DVFNNLCPLLLKSKNFESEHVPEFRSITTRLLKDIIPDYEAKVMAFLHKNNNPMRYSE
                     RDISEQGALLGLSDEKIDVVQNEMRERKALEVQNNNRSQAIELKKTLIGAVDEYLRWR
                     NNESKETDYQKSSGAFTWLRHYTDFGKNRANDLKNELNKAQDLKTMLDVLQKHFANNS
                     RLHNHSLDSYLLRAVYKDFNKFNSIFNFEHLAIKNDTDANREWLREEMLGMVAKTNMN
                     VTLEKSINSRQESPTLPNKTKVHISIMKIPANEREENILAVHTALRNDELLRNQDGSV
                     PAIIKEIRDIVGKIDPSEEENIANAIIEIKRTIADNSDNNYNENAHDIIKAFENPSCC
                     DFRKIRAALTTNHAIDEIMNPVRVGMQLN"
     gene            complement(3961..4113)
                     /locus_tag="I9760_002740"
     CDS             complement(3961..4113)
                     /locus_tag="I9760_002740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044486.1"
                     /translation="MSISSCIDMANHKQSTKQAMLDKITLFRIMAVSELSKLLINPNN
                     SVLVGA"
     gene            4261..4824
                     /locus_tag="I9760_002741"
     CDS             4261..4824
                     /locus_tag="I9760_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044487.1"
                     /translation="MTIYFLFGKTGSGKSYIGELLRQLSIVHIDGDNHITQKMRDCLI
                     EDEQMTPEMIDEFVDVLIDVIKTQKTKTPNESFVISQAMYLDKHRLKLLNAIPELEFV
                     MIDVIPVLRASRITNRFRNQESKVSLRYATRMDAFFEYPSHETIRLENNQSSDETLIE
                     QIREKMPALFNMESKEELNSGIQLQFA"
     gene            complement(4939..>5033)
                     /locus_tag="I9760_002742"
     CDS             complement(4939..>5033)
                     /locus_tag="I9760_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HAT5044488.1"
                     /translation="NDTGERSYFGDSAGLSNKHYTVALGLQYLF"
BASE COUNT         1659 a          977 c          952 g         1445 t
ORIGIN      
        1 taacccctcc ttttgagcaa agataaattt aatgagagag ccaactcaat gccctcaaca
       61 agaaacattg aaacgcataa cgactccaat cccacattgc gagcactaag cctatcagcc
      121 ttgggcatag tttacggaga tattggaacg agccctcttt atacttttaa gacggtgata
      181 ttgctggctg gagggggaac tccagccgta aacaccatta tgggttcggt atcattgatt
      241 atatggactc tgataatcat cgcatctgtt aaatacatcc actttgcttt acggattgac
      301 aacgatggtg agggagggat tctcgcgcta atgtcccttt tgagtttaaa actgaagcaa
      361 aggcctttca ttatcgcagt tggacttatg ggggctgctc ttatctatgg agatggcaca
      421 attaccccag ccatatcggt gctctctgct gtagagggac ttgaaatcct ttccccatca
      481 cttaagtatt atgttttgcc tattgcgata acaattctga taacactttt tgcgattcaa
      541 tctaaaggta ccgcaactat tggaaaggcc tttggcccag tgatggcatt ttggttttta
      601 accattggta ttttaggggc aagaggagtt atccaacacc cttctgtatt agcggcgatt
      661 aatcccatct atggcctaag ttttttattt tcaaatggcg ccacaggatt ttttattcta
      721 tgtggggtct ttctttgcgt cactggtgca gaggctttat acgccgacct gggacatttt
      781 ggtaccgccc caattcgctg cgcctggttt ggtctggctt ttccaagcct tatctttaac
      841 tatctgggac aagccgcttt ggttttggaa ggagcatcca cggagcataa tattttttat
      901 atgctgtgcc cgggtgattt tttattaccg ttaataattc tgtccaccgt cgcaacaatt
      961 attgccagcc aagccattat taccggagca ttttccatga caagacaagc aatgcaactg
     1021 ggttggctgc ccagattacg agtcacgcaa acgtcttcag aaggttatgg gcaaatttat
     1081 ataggagtcg ttaactggct tttaatgctg gctacccttg ggcttactat tggatttggc
     1141 agttctgaaa aattagccgc tgcctatggc attgctgttt cagctactat gctttgcacc
     1201 actctgcttt tatttattgc tctgcataaa ctatggaaat gggatattat cacatcaggg
     1261 cttgttgctg gattattcat gattgtcgat gcctcttttt ttgcggctaa tctcacgaaa
     1321 ttcatcaatg gcggatatat accaatcaca ttggcaatca taatctattc catgatgtat
     1381 atttggcata aaggctatca aaccatcgca atcaagcaaa aagaaaaaaa tataacagtc
     1441 gcttcctttc tggacagtat tcaaaaagag agggtagtcc gtgtgcctaa aactgctgtt
     1501 tttcttacat ccaaagagca ggatattcct ccaatcctgg tttggcatgt caagaaaaat
     1561 catgtgttgc aggataaggt gattatttta aaaattaaca acttgtccat tccccggtgt
     1621 aaacctggtg atcgattaca aattgtagaa acaggaacag gcatctggca tgccgtagca
     1681 aattatggct ttatggaaca acctcatatt ccaaaattat taaagaaatt agaggctcaa
     1741 ggctatgaca tcaatatcaa agacataacc tattatatcg gtcatgaaac aatatttgtc
     1801 cgcaatgtcc gtcatactct gtcaaaatac attaaaattc tcttcgtttt tatgcatcgc
     1861 aatgcccttc ccatgagtaa ctactttcat cttccgccag aatcagtatt tgaaatagga
     1921 cgacagatag aaatttagtg aaaataattg atcccacaca tggaaaactc tgtgggtgag
     1981 tgctgtttta tggtcaggat tgaattttgc acctggtctt atagcacata tgacaaatca
     2041 agattaaatc ctggcttttg ctcttttgca gattataccc aggtcccctg aaaaagatta
     2101 taaacatacc aaaaaagtga cctataatta taaaacaaat actttggatg atagaaatgg
     2161 ttcaatttgc caaaccatct attggccctc cggctgattg tggacgaata aatctcttaa
     2221 ttggacgtga gttagggcaa tatcaaaata tcatacaaaa atggattgct aatctcgaag
     2281 acaacgcgaa acaaagacct tctaatattt ctgaatttaa atttggaaaa actttaggat
     2341 atcttttact tcaaagctta aagaaagcaa atttacatcc agacaacttc agatcttttg
     2401 gtttcgaccc ttgggaagaa cctcattatc aatcggcact aatcgctgga aaatatgtga
     2461 taactcaaga tacgtaccga atttactttg atataccaaa aattgatcat tcagatgagg
     2521 aaaaaataaa ggctaatcaa gaccatgcgc agatacttta tttcacaact atgaccaatg
     2581 atggccttat ggtttttacc tttgaagata aagtagaaaa tatcaattcc agattatttg
     2641 tagaactagc aaaaaacata aagataaact cagaagcatg gagtcaatta atacagttta
     2701 cagaaaaaaa tcttttatat gaacaagatg atatttcatc aaaattgccg caagtatttg
     2761 gtttgatatt atatgcaagc atatctccag aacacaggga agatgttttt aataaccttt
     2821 gcccattact cttaaaatca aaaaactttg aatcagaaca tgttcccgaa tttcgttcaa
     2881 ttactacaag actgcttaaa gatattattc ctgattatga agccaaagtg atggccttcc
     2941 tgcataaaaa caacaatcca atgcgttata gcgaaaggga cattagtgag caaggagctc
     3001 ttttaggtct ttcagatgaa aaaattgacg tagtacaaaa cgaaatgaga gaacgtaaag
     3061 cattagaagt acaaaacaac aatcgatcac aagccattga actaaaaaaa actctcattg
     3121 gtgccgtaga tgaatacctt agatggcgca ataatgaaag caaagaaacg gattatcaaa
     3181 aaagctcagg tgcttttacc tggctgcgtc attacacaga ttttggcaaa aaccgagcca
     3241 atgatttaaa aaatgagctt aataaagcac aagatttaaa gaccatgctt gatgttttac
     3301 aaaaacattt cgcaaataac tcccgattac ataatcattc tcttgatagt tatctattaa
     3361 gagcagtata caaagatttt aataaattca actcaatatt taattttgaa catcttgcaa
     3421 ttaaaaatga taccgatgct aaccgtgaat ggctaagaga agaaatgttg gggatggtag
     3481 caaaaacgaa catgaatgtt acattagaaa agtcaataaa tagcagacaa gagagcccca
     3541 ctctgcctaa taaaacaaaa gtacatatca gtatcatgaa gattcctgct aatgaacggg
     3601 aggaaaatat tttggcggtc cacaccgcgt taagaaatga tgaattgctg agaaatcaag
     3661 atggatccgt tcctgctatc ataaaggaaa tacgagatat tgtagggaaa atcgaccctt
     3721 cagaagaaga aaacatagcc aatgcgatta ttgaaatcaa aagaacaata gcagacaaca
     3781 gcgacaataa ttacaacgag aatgcgcatg acatcatcaa agcatttgag aatccaagtt
     3841 gttgtgattt tagaaaaatc cgcgctgcct taacaaccaa ccatgcaatc gatgaaataa
     3901 tgaatccggt cagagtagga atgcagttga actaattcaa gctatttttc tacccctcag
     3961 ttacgctccg accaaaacgg agttgttcgg attgattaaa agtttgctta actcggaaac
     4021 agccatgatt cgaaataggg ttattttgtc caacatagcc tgtttggtgg attgtttatg
     4081 gtttgccata tcaatgcagg aagaaatact cattatggtg ataatatcct ttggcagatt
     4141 ggttataagc ccatcctctc tagtcaggta ataaccaaga cagtacacat ttccatgatt
     4201 ttaaggtatg gttattcact tacctaatat gaaaaacata cattacaagg gctgtgatca
     4261 atgaccatct actttttgtt tggcaaaact ggctcgggca aaagttatat tggagagctg
     4321 ctcagacagc taagcatcgt ccatatcgat ggtgataacc acatcaccca aaaaatgcgt
     4381 gactgtctaa tcgaagatga acaaatgact cctgagatga ttgatgaatt tgtcgatgtc
     4441 ttgattgatg ttatcaagac acaaaaaact aaaacaccga acgagagttt tgttatttca
     4501 caagccatgt atctcgacaa gcaccgtctc aagctattaa acgccatacc cgagctcgaa
     4561 tttgtaatga ttgatgtcat tcctgtactg cgggcaagcc gcatcaccaa tcgatttcgc
     4621 aaccaggaaa gcaaagtaag cctgcgctat gccaccagga tggatgcatt ttttgagtat
     4681 ccttcacatg aaacaatacg gcttgaaaac aatcaaagct cagatgaaac gcttatagag
     4741 caaatccgtg aaaaaatgcc ggcactgttt aacatggaat caaaagaaga attaaactca
     4801 ggcatacagc ttcagtttgc atagcttgag agccatatcc agatattgtt gaaataacga
     4861 acgcatgcag caaccgtctt gaagccagaa cttaagaagg ttgccacttg ctagttgaga
     4921 ttagagaatt tgagcctttt aaaaaagata ctgtaaacca agagcgacag tatagtgttt
     4981 attggatagc cctgctgaat caccaaaata actgcgctca cccgtgtcat tgt
//
LOCUS       DACTJE010000199         4991 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.201, whole genome shotgun sequence.
ACCESSION   DACTJE010000199 DACTJE010000000
VERSION     DACTJE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.201"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1556
                     /locus_tag="I9760_002743"
     CDS             <1..1556
                     /locus_tag="I9760_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044489.1"
                     /translation="ELSNYNENPTVLITQSQSGNSRSQHAVWGNTTTVAELKEGSSYQ
                     FSTPAIQYNDYNCWPTFTPSSLVASAANVPTTNLTYQCAQIVKDNVTINVSGAPSSLA
                     SLKIILTPNDGSQTVEQTIDLANGVGSSAISLTDGGIYTLSSEGVPGYTVQFSPQPLT
                     ATENATVNITLSPVTAAKGRIIGYVPGWKTPPAAQELASAGYTHVMIAFGVFSTNTPG
                     VIVPAFETITKEYIQSLHQVGIKVILSLGGALTSIPNTTVDFHQVLVASSSPEAFKQT
                     FINSLKELISQYDFDGFDIDIEHGINAGGSFSQPQGDIAVLASIINTMYSQNPSLLIT
                     LTPQVANIAATSGFDQTWGNYASLTMQTHQSLAWVGIQLYNTGCAFGIDQVCYGPTPT
                     DTPDFSVAMATDLLENWPATVNGRPTGFQPYVSYLRPSQIVIGYPSPNSNGGSDGSPV
                     TPTTTIKRAIQCLKTAIASNTSCGAYAPPRAYGNIGGVFNWEVTYDKNNQFKFAKELK
                     NCVINGVCE"
     gene            complement(1595..2068)
                     /locus_tag="I9760_002744"
     CDS             complement(1595..2068)
                     /locus_tag="I9760_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5044490.1"
                     /translation="MAIYKFEISTMQNGNIQISAKHKLTGEIHGYVQLASSDRLESRN
                     LLEIDQLYVDNNARGHGLGSELASRAIKYALKMDKGIHMMIQPGSDVFWKKYFNSRFD
                     GKRITLFGGEALNTNLADGKVFEISKESLVANYKFPQDRHLRDRDMERICTKLSN"
     gene            complement(2160..3275)
                     /locus_tag="I9760_002745"
     CDS             complement(2160..3275)
                     /locus_tag="I9760_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAT5044491.1"
                     /translation="MSRYVCKWKLIYTFFIFIVFNNCPLAFASPSLTKQLHTILKDNL
                     ADSLTPGAVLLISSADLGTISVAAGLADKENDISMNITDSFRLASMSKTFLAVTVLKL
                     VADKQLNLDDHIADLLPDSIDIERIPNGDEVTVQQLLQMRSGIPNYTDYDSYSDLIDT
                     MADKEWTPEECIKLVYDQKPDFKPGTAYEYSNTNYLLLQLIIENITGESYAESIKKYI
                     LKPLHLKNTYIEKQEFDDDHHYLSTHGYTLEDGKVVDVTDHDDGFGLADGGIISTAED
                     INIFVQALLKDKILLSPNYLTMMLTFTDDYGFGIGREEINGEIAWSHNGASSGYQGQY
                     YYFPDRQLTVVILTNYFDSDIIEDIVSQTLSAIDNSE"
     gene            3543..4607
                     /locus_tag="I9760_002746"
     CDS             3543..4607
                     /locus_tag="I9760_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HAT5044492.1"
                     /translation="MVNKICLSFALLISVPSILLAEDKLIFAVDIIRHGDRTPIVALP
                     TVNYQWQEGLGQLTAEGMQQEYKMGVAFRKKYIEESHLLPEHYEYGTIYVRSTDYART
                     LMSAQSLLMGLYPPGTGPTIPAGTSALPHAFQPIPVFSAPSKYDEVIIQQVDRKEREK
                     LMEQYVFSTREWQQKNNELKDKYPLWSRLTGINIDNLGDLETVGHTLYIHQIHNAPMP
                     EGLASNDIETIINSAEWAFMAQEKPQQIANVYSSKLMTNIADYLNSGSMKKSKLKYVL
                     LSAHDTTIASVLSFLGAPLEKSPPYASNVNFSLYDNGANYYTVKITYNGNPVSIPACG
                     GSVCELQQLINLVHDSKNSV"
BASE COUNT         1499 a          977 c          928 g         1587 t
ORIGIN      
        1 gcgaattaag caactacaat gaaaatccaa ctgttctaat tacccagagt caatcaggaa
       61 attcacgctc acagcatgcc gtttggggga acacaacgac tgttgctgag ttgaaagagg
      121 ggagtagtta ccaattctca actcctgcaa ttcaatataa cgattacaat tgctggccca
      181 ctttcacacc atcttcctta gtggcaagtg ctgcaaatgt tccaactacc aatttaacct
      241 atcagtgtgc ccagattgtg aaagataatg tgacaattaa tgtcagtggc gctccatcct
      301 ccctggcttc attgaaaatt attttaactc ccaacgatgg ttcacaaaca gtagaacaga
      361 caatagattt ggccaatggt gttggttcat ctgccatttc tttaactgac gggggtattt
      421 atacactttc aagtgaaggt gttcccggct atacagtaca attttcgccc caacctttaa
      481 cagcaacaga aaatgcaaca gtaaacatca ctttatctcc ggtaaccgct gcaaaagggc
      541 gtattattgg ttacgtaccg ggatggaaaa ctccacctgc cgctcaagag ttggctagcg
      601 cgggttatac tcatgtcatg attgctttcg gcgtatttag taccaatact ccgggtgtta
      661 ttgttcctgc ttttgaaaca ataaccaaag aatatattca gtctcttcat caagtcggga
      721 ttaaagttat tctttcttta ggtggtgcgt taaccagtat tcccaataca acagtagatt
      781 ttcaccaggt tttagtagct tcttcttcac cagaggcatt taaacaaaca tttatcaact
      841 ctttaaagga gttaatttct caatatgatt ttgatgggtt tgatatagat attgagcatg
      901 gtattaacgc tggcggttcc ttttctcaac cacaaggtga cattgctgtc ttagcaagca
      961 ttattaatac gatgtacagt caaaatcctt ctttgctgat tactctgaca cctcaagtgg
     1021 ctaatattgc tgcaacaagc ggtttcgacc aaacctgggg gaattatgcc tctttaacta
     1081 tgcaaactca tcagtcattg gcgtgggtag gcatccaact ctacaataca ggatgtgctt
     1141 ttggtattga tcaggtatgt tatggcccta caccaactga tacgcctgat ttttcggtag
     1201 ccatggctac cgatttattg gaaaattggc cagcaaccgt caatggacgt cctacaggat
     1261 ttcaacctta tgttagctat ttaagacctt cccaaattgt aattggttat ccctccccaa
     1321 attctaatgg tggcagtgac ggttcaccag tcactccgac aacgacaatc aaacgggcta
     1381 ttcagtgcct taagacagca attgccagta ataccagttg tggtgcttac gctccaccaa
     1441 gagcttatgg aaatatcggt ggtgtattta actgggaagt gacttatgat aaaaacaatc
     1501 aattcaaatt tgcaaaagaa ttgaaaaatt gtgttattaa tggtgtttgt gagtaattca
     1561 tttgaccaag tcaggcttta gagcctgaca cttattaatt agagagtttg gtgcagattc
     1621 tttccatatc gcgatcacgc aagtgtctat cttgaggaaa tttgtaattg gcaaccagac
     1681 tttctttaga aatttcaaat acttttccat cagctaaatt agtatttaaa gcttctccac
     1741 caaacagggt aatgcgtttt ccatcaaaac gagaattaaa atattttttc caaaacacat
     1801 ccgaacctgg ttgaatcatc atatgaattc ctttgtccat tttaagagca tatttaatgg
     1861 ctcttgaggc aagttcactt cccaatccat gtcctctggc attattgtca acataaagct
     1921 gatctatttc taacaaattt ctgctttcta acctgtccga tgaggcgagt tgaacatagc
     1981 cgtgtatctc tcctgttaat ttgtgctttg ctgatatctg tatattgccg ttttgcatag
     2041 ttgagatttc aaatttgtaa atagccatat tttgccccca taaaagccta gataattttc
     2101 acttagtata acatataatc tattttgcgg gcttccatta gatgagtgca gctaagcagt
     2161 tattcagaat tgtcaatcgc actgagagtc tgagatacta tgtcttcgat gatatcactg
     2221 tcaaaatagt tggttaaaat aacaacagtc aattgccggt ctggaaaata gtagtactgg
     2281 ccttggtatc ctgagcttgc cccattatgg gaccaggcta tctctccatt aatttcttca
     2341 cgtcctattc caaaaccgta atcatctgta aaggtaagca tcattgttaa atagtttgga
     2401 gataaaagta ttttatcttt caacaacgcc tggacaaaga tattaatatc ttcagcagta
     2461 ctaattattc caccatcagc caagccaaaa ccatcatcat ggtctgttac gtcaaccact
     2521 ttgccatctt ccaaggtata gccatgtgtt gataaataat gatggtcgtc atcaaattct
     2581 tgcttttcta tatatgtatt ttttaaatga agaggtttca ggatgtattt cttaattgat
     2641 tcggcatagg attctccggt tatgttttcg ataattaatt gcagcaacaa ataattagta
     2701 ttgctgtact cataagctgt tcctggctta aagtcaggtt tttgatcgta gactagttta
     2761 atgcattctt ctggtgtcca ttccttgtct gccatagtat caatgagatc actgtaagaa
     2821 tcataatccg tataattagg aatgccgcta cgcatttgta gtaattgttg cactgttacc
     2881 tcatctccat tcggtatacg ttcaatatct atactatctg gcaataagtc tgcaatatga
     2941 tcatcaagat ttaactgttt atctgctacc agttttaaga cagttacagc caaaaatgtt
     3001 ttactcatgc ttgcaagacg aaaactgtcc gtaatattca ttgaaatatc attttcctta
     3061 tctgccaacc ctgccgcgac ggaaattgtg cctaaatcag cacttgatat cagtaatact
     3121 gctccagggg ttaatgagtc tgcgagattg tcttttaata tggtatgtaa ttgcttggtt
     3181 aaggaagggc tcgcaaaggc aagtgggcaa ttattaaaga caataaaaat gaaaaacgta
     3241 taaatcaatt tccacttgca tacataacgg ctcattgcaa ctccaatgtg gcaagggttt
     3301 tctcacccac caaatgagct atatcttaat tctagtcgat ttcaggataa tttctttact
     3361 tcctgtataa aatagattgt tcctccaggt tcaaaatgaa tcaaggaata caaaagccga
     3421 tgtcttgatt tttgtttgtg tcttcgtcat agtgcgaaac cgagattaac caacggtggc
     3481 aagatacgac aagaattttt tacgaagcct catgtttact ttaactcaag gagtatgaat
     3541 acatggtcaa caagatttgc ctgagtttcg cactattaat tagcgtaccc tccattttat
     3601 tagcagaaga taagcttatt tttgctgtgg acattattcg tcatggcgac agaacgccga
     3661 ttgttgcttt gcctactgta aattaccaat ggcaagaggg gcttggccag cttacggcag
     3721 aaggtatgca acaagaatac aaaatgggtg tggcatttcg caaaaaatat attgaagagt
     3781 cgcatttgtt acctgaacac tatgaatacg gaaccatcta tgtccgctct acggattatg
     3841 cacgtactct gatgagcgcc cagtcattgt taatggggct atatccacct ggaactggac
     3901 caacaattcc tgcaggaact tccgctttac ctcatgcttt tcagcctatt cccgtattta
     3961 gcgcaccgtc taaatacgac gaggttatta ttcagcaggt agatcgtaag gaacgggaaa
     4021 agctaatgga gcaatatgtt ttttctacca gggagtggca acaaaaaaat aatgaattga
     4081 aagacaaata cccattatgg agccgtctga ctggtattaa tattgataat ctgggggatc
     4141 tggaaacagt tggtcatacc ttatatattc atcaaatcca taatgctcct atgccggaag
     4201 gattagcttc taacgatatt gaaacaatta tcaattctgc tgagtgggct tttatggcac
     4261 aagaaaaacc tcaacaaata gcgaatgttt atagttcaaa gctgatgact aacatagctg
     4321 attatctgaa ttccggaagc atgaaaaaat ctaaactgaa atatgttttg ctatctgctc
     4381 atgatactac aattgccagt gtattaagtt ttttaggtgc tcctttggaa aaatccccac
     4441 cttatgcctc aaatgttaat ttttctctct atgataatgg agcaaattat tacacagtaa
     4501 aaattaccta taatggtaat ccggtttcta ttccagcgtg tggtggttca gtttgtgagt
     4561 tacaacagct tataaatttg gtacatgatt ctaaaaattc agtgtaacta ctcattctgg
     4621 aggtatttgg ccgaggtaag taatcctaac catctcaaac tatctgttgt tgcaaaacag
     4681 caatagcagc tggtttgaga taattcaatt aatcgatgaa ttccaaaaga tctgggcgca
     4741 tgaaagggat actctaataa tcaagattct gttcatggta agatgtgttg tggtcgtgct
     4801 cggaatgcaa tccttgatag agtacacaca aatctggatg gaaaaattta taaattaaat
     4861 atgacctgac agataccttt gaatagctgg taactgtcag atcaaacttg aataactaag
     4921 tcttatattt agctaagatt agtttttagt cttagtttca agattcaact tttgatttaa
     4981 atcaatatct a
//
LOCUS       DACTJE010000200         4982 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.202, whole genome shotgun sequence.
ACCESSION   DACTJE010000200 DACTJE010000000
VERSION     DACTJE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.202"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            95..1180
                     /locus_tag="I9760_002747"
     CDS             95..1180
                     /locus_tag="I9760_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HAT5044493.1"
                     /translation="MKNLYIDPDITKASTISSEFYTSTEWFEITKEKIFSKTWQFCIS
                     TEELTTNRQLIPFTLLPGYLEEPLLFAKDELGTIRCLSNVCTHRGKILIEEPCVAHKI
                     KCSYHGRRFNFCGELLHMPEFELTKDFPSNNDNLSQISTGILEPFLFASLSPVVPFHE
                     VFAEIKERLFWLPMEKMRLDKEKCKDYVVKSHWALYCENYLEALHIPFVHPSLRKVID
                     CNTYTTELFRYCNLQLALASPDEVSFDLPKGAQDYGKQVAAYYFWIFPNTMLNFYPWG
                     CSVNVVKPLSPELTQVSFLSFVLDESKFGKGAGGALDQVEQEDERVVESVQRGIKSRY
                     YDIGRFSPTKEQGTHHFQRLLCEFLND"
     gene            1341..2462
                     /locus_tag="I9760_002748"
     CDS             1341..2462
                     /locus_tag="I9760_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044494.1"
                     /translation="MAAQRHLYIFTLIGLLLGVVVDILIRYNNTTAFIYSVVTIFGVL
                     FALTYNNVNFSRLIGTSFLLAFFLSIPLFPLKMDYSMKDYFHFFTFFVGFPFFIYVAH
                     CFHYAYHHDNTWRVSYSSLFAGVWNTIPLLFIALVFSSLANLLIVLGSFVFKTVGNNY
                     LWDLYFYNRDFKLISNTTLFFMGLGVGQQNLNIIHNMRFLLLRIMYYLFPLLAAISIL
                     YFILYTFHSFSSSQEHINPLIVLIPLTTAGIIFFNAYFQDGTIKSDYPSWLKLSLRVY
                     RVILFLLALMMTYKILSDFSLDTNAFIYLLVAVLFSFTYAITAFLNENQEKQWIYMGN
                     IGTAIFFIVTLFLCNLPYIPVEFTIGGGNAINFITSTLS"
     gene            complement(2528..4027)
                     /locus_tag="I9760_002749"
     CDS             complement(2528..4027)
                     /locus_tag="I9760_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044495.1"
                     /translation="MKKIVYVLLFISSLTHANCVIYFNEQPLNKRLSDPLYSLLSNTE
                     ICPQSIQELRNSMRLRGLKEVIGMVANRGRNNPVEGSFSFFESVYGRLATNQLIAKGE
                     LFLGYFTRLKDGLIILDQKPRKGKLLIEAISWDAKKALYNFYELRGIEHGGVRWFYRG
                     NSKDAYKDNTWLYRISPEGENHFGSRMRCSACHNSGGPILKEQSLPHNDWWTNQRPLI
                     LLPNKPDGDVSQLLEQISDASVFANDVITGMRHLSDSKKMNAFQGKLSLQEQLRPLFC
                     TTEINLQSSTDSSETVVSIPSAFWLNPLLGDIHLTISMEDYNQLLKEFGTRFPETSLQ
                     DADHAWLTPVKGEADLNAIKQLIQHNIISKKFAQSVLMIDFTNPLFSSKRCELLKLIP
                     ESYQTNSKEWVEQFLINLRNSEEQFKGARQLADYMSKEQLDSKELMKKIKIYEYFLNQ
                     SISTRSGLRKHFQHLIELRKAVFRSELSQNPLGQILEPGFRVIFPESQQ"
     gene            complement(4170..4889)
                     /locus_tag="I9760_002750"
     CDS             complement(4170..4889)
                     /locus_tag="I9760_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044496.1"
                     /translation="MTERKKTTVKNKKAPVKKSQLTVSLLIFIDLISILLSLMPGVAA
                     FSLTGLFLYYGFSKTWLVLICSPFVLLFSFILIIALIRISLPKLKPGRYKRELNKTMI
                     AWFCHFALSRAAKIVGLIHLLQSFNVIKYLYWRALGIKVSFRVMNSFDIDFVDCPLIS
                     IGKDVTLASQVSISCHSDVGNYLFLSPVVIEDNVFVGMTTKIGPGTTIKKGAWIGYGN
                     AFVNQVIEENAKIGNVRPVPE"
BASE COUNT         1523 a          966 c          839 g         1654 t
ORIGIN      
        1 tttatcagac tttaaccatc gcttcaccga aagaaatggt taaaattttt attctctttt
       61 tgcaggtttt tctctttacg gtaaggaaat gttcatgaag aatctctata tagatcctga
      121 tattacaaaa gcttccacaa tctcttctga attttataca tcaacggaat ggtttgaaat
      181 tacaaaagaa aagatattta gtaaaacctg gcagttttgt atcagtaccg aagaattaac
      241 aactaatagg caattaattc cttttacctt gctgcctgga tatcttgaag aacccttact
      301 gtttgcaaaa gatgaattgg gtacaattcg ttgtttgagt aatgtatgta cccatcgagg
      361 taaaattctc attgaagagc cttgcgtggc gcataaaatc aaatgctctt atcatggccg
      421 tcggtttaat ttttgtggcg aattacttca tatgcctgaa tttgaactca caaaagattt
      481 cccatcaaac aatgataacc taagtcaaat ttcaacgggt attcttgagc cttttctctt
      541 tgcttcgctc tcaccagttg tcccatttca tgaagttttt gcagaaatta aagaaaggtt
      601 gttttggctc cctatggaaa aaatgcgcct ggataaagaa aaatgcaaag attatgtagt
      661 taaatcgcat tgggccttat actgtgaaaa ttaccttgaa gcgttacata tcccttttgt
      721 acatccctcg ctaagaaaag taatcgattg caacacctac accacagagc tcttccgcta
      781 ctgtaatctg caattggctt tggcaagccc tgatgaagtc agctttgatt tacccaaagg
      841 ggcgcaagat tatggcaaac aagttgctgc ctattacttt tggatattcc ccaatacgat
      901 gcttaatttt tatccctggg gttgttctgt gaatgtagtc aaaccactta gtcctgagtt
      961 aactcaggtt tcctttttat cttttgttct tgatgaatca aaatttggga aaggcgctgg
     1021 tggagcactt gaccaggttg aacaagaaga tgaaagagtg gtggagtctg tgcaaagggg
     1081 gataaaatct cgctattatg atatagggcg tttctcccca acaaaagaac agggcactca
     1141 tcattttcaa agactacttt gtgagtttct taatgattga aattagatct gtcacatgct
     1201 cagacttgga acgaaaaaat atttttaata aacgatttag atcaattaag taactgagtt
     1261 agaattattt ttagataacg attagggcat tctactaaac cctaaatcat gaatataacc
     1321 cccttgtgga gaaagacagg atggctgctc aaagacacct ttatattttt accctgattg
     1381 gattacttct aggggtagta gtggacatac tgatacgcta taacaatacg actgcattta
     1441 tatacagtgt tgtcactatc tttggtgttt tatttgccct tacttataac aatgtcaatt
     1501 tttcgcgtct gattggaacc agcttcctac tcgctttctt tctatcaata ccattgtttc
     1561 cgttgaaaat ggactactca atgaaagact atttccattt ttttactttt tttgttggct
     1621 ttccattttt tatctacgtc gctcattgtt tccattacgc ttatcatcat gacaacacct
     1681 ggcgggtaag ttacagcagt ttatttgctg gagtatggaa tacaattcct cttttgttta
     1741 ttgccctagt cttttcctca ttggccaatt tacttattgt attgggatct tttgtattta
     1801 aaaccgttgg aaataattat ttatgggatt tgtattttta taatcgtgat tttaaattaa
     1861 tcagtaatac aaccttattt tttatgggtt tgggtgttgg tcaacaaaat ctcaatatca
     1921 ttcataacat gcgattttta ttacttagaa taatgtatta cctgtttccc ctcttggcag
     1981 caataagtat cctctatttt atattatata catttcattc tttttcgagc agtcaggaac
     2041 acattaaccc gttaatagta ctaattccac tgactacagc aggtatcata tttttcaatg
     2101 cctattttca ggatggaact attaaatcag actatccttc ctggttaaaa ttatctttaa
     2161 gagtctatcg agtcattctg ttccttctgg ctttaatgat gacttataag atcctttctg
     2221 acttctcact agatacaaat gctttcattt acttattggt tgccgtacta ttcagtttca
     2281 cttatgccat tacagcattt ttgaatgaaa accaggaaaa gcaatggatt tatatgggaa
     2341 atataggtac agcaatattt ttcattgtca ccctgtttct gtgtaatctg ccctatattc
     2401 ctgttgaatt cacaattggt ggaggaaatg caatcaattt catcacctca actctttcgt
     2461 agaaaataat actcagcgca tggcagtttt ttgtcatgca cagagccaat aatagacctc
     2521 ttcttgtcta ctgctgactt tcagggaaaa tcacccgaaa ccctggttct aaaatttgcc
     2581 ctaaggggtt ttgtgacagt tcactacgaa agaccgcttt tcgcagctcg attaaatgct
     2641 gaaaatgttt ccttaatcct gatctggttg atatagactg attaagaaag tattcataaa
     2701 ttttgatttt tttcattagc tcttttgaat ccaattgctc tttactcata taatcagcta
     2761 gctgacgagc gcctttaaat tgctcctcac tgtttcttaa attaatcaga aattgttcaa
     2821 cccattcttt gctgtttgtt tgataggatt ccggtattaa ttttaataac tcacatcttt
     2881 ttgatgaaaa caaaggattg gtaaaatcga tcatcagcac tgattgtgca aattttttgc
     2941 taattatatt gtgttggata agttgcttaa tcgcgttgag atcagcctcc cccttgacag
     3001 gtgtaagcca ggcatgatca gcgtcttgca aagacgtttc tgggaaacga gttccaaact
     3061 ctttcaatag ttgattataa tcttccattg aaatggttaa atgaatgtcg cctaatagtg
     3121 gattaagcca aaacgctgat ggaatggata caacggtttc tgatgagtca gtgctggact
     3181 gaagatttat ttcggtagta caaaataaag gccgtaactg ctcctgcaat gacagtttcc
     3241 cttgaaatgc attcattttt ttactatcgc tcaaatgtcg cattcctgtt atcacgtcat
     3301 ttgcaaatac actggcatca ctgatttgtt ccaataattg gctcacatcg ccatcgggct
     3361 tattggggag caaaataagc ggacgttgat tagtccacca atcattatga ggaagagact
     3421 gttccttaag gatcggcccg ccggagttgt ggcaagctga acatcgcata cgactgccaa
     3481 aatggttttc gccctctgga cttattctat ataaccaggt gttatcttta taagcatcct
     3541 tcgaatttcc tcgataaaac caacgtacgc ctccatgttc tattcctcgc aattcataaa
     3601 aattataaag cgccttttta gcatcccagc tgattgcttc aatcaataat ttacctttcc
     3661 tgggtttttg atccaaaata attaggccat cttttaaacg agtaaagtac cccagaaata
     3721 actccccctt tgcaatcaat tggttagttg ctaatcgacc atacactgat tcaaaaaaac
     3781 tgaaactgcc ttcaacagga ttatttcttc ctctattagc gaccatccct atgacttctt
     3841 ttaatcctct caatctcatg gaatttctta actcctgaat cgattgcggg caaatttcag
     3901 tattggacaa cagagaatac aaaggatcag ataacctttt attaagcggc tgttcgttaa
     3961 aatagatcac acaattggca tgagtcagcg aagaaataaa taacaagacg taaacaattt
     4021 ttttcattct ggaacatccg tttaatcaaa gaaataatca gcggcttcaa agcctgattg
     4081 cagtaatctg gattcattaa gttaaccggg aaaacaatgc actaaattct gatttttatt
     4141 aatccaaact taagataaaa catttcattc tattcaggaa caggtctaac attgccaatt
     4201 tttgcatttt cctcaataac ctggttaaca aaagcattcc cataacctat ccaggcccct
     4261 tttttaattg ttgttcctgg gccaatttta gtcgtcattc caacaaaaac attatcctct
     4321 atcaccacag gcgataaaaa aagataattt cctacatcag aatgacaact gatagagact
     4381 tgactggcaa gggtaacatc ctttccaatc gaaatgagag ggcaatccac aaaatcgata
     4441 tcaaatgagt tcataacgcg aaatgacact ttaattccca atgcacgcca atacaaatat
     4501 ttaataacat taaaggattg taataaatga atcaaaccaa caattttcgc tgcacgagag
     4561 agtgcaaaat gacaaaacca ggcgatcatg gtcttattta actctcgttt atagcgacct
     4621 ggcttaagtt ttggaaggct tatcctgata agtgcaatga taagaataaa agaaaaaagt
     4681 aatacaaacg gtgagcaaat taaaacaagc caggtttttg aaaacccata atacaaaaac
     4741 aaaccggtta agctaaatgc agccacgcca ggcatcaaac tgagcaatat agaaatcaaa
     4801 tcaataaaaa tcaataaaga aacagtcagc tgactttttt tgacaggtgc ttttttattc
     4861 ttgactgtcg ttttcttgcg ctccgtcatc ctgtgatttc cttgaaaata atagacaatt
     4921 catttgccaa cctttgaggt tgttttattt aaacctggag gtatactaac aaaaacaagt
     4981 aa
//
LOCUS       DACTJE010000201         4737 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.203, whole genome shotgun sequence.
ACCESSION   DACTJE010000201 DACTJE010000000
VERSION     DACTJE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.203"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..310)
                     /locus_tag="I9760_002751"
     CDS             complement(<1..310)
                     /locus_tag="I9760_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT5044497.1"
                     /translation="MIDDPIDLLRQYSRKLVRELGMLQLNKLAAKEQPSYWHTLIEIN
                     KEPNITISKLSQLLLVSLPTLSRIVNSLINDELVTVNEGLDKRERFLRVTDKGKEKVN
                     Y"
     gene            599..1237
                     /locus_tag="I9760_002752"
     CDS             599..1237
                     /locus_tag="I9760_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAT5044498.1"
                     /translation="MEQWHQFTLLFPLIILVFISLISPGPDFAITVKNSLAYSRKSGL
                     LTALGIALGSLVHISYTLLGLGLFITKTPIILHIIQYLGAGYLFYIGLKNLGAKPNAD
                     QIGSYTTNNDLKPSEAFFNGFITNLLNPKAMLFFISLFSVLMPREISISTKLFLAIVL
                     FLQTLLWFSFVAFSLSGKNTRTKFQRISYWIERITGVILIALALKLLLSQLV"
     gene            complement(1267..1815)
                     /locus_tag="I9760_002753"
     CDS             complement(1267..1815)
                     /locus_tag="I9760_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5044499.1"
                     /translation="MKYLTTSRLLLRAWDREDTIPFYRMSQDPRVMKYFPELWSMDMV
                     KDFIIRMNEQLSQKKFTLWAAEVKDNKQFIGFIGLNAPTWNAHFTPCVEIGWRLATEF
                     WGQGYATEGAKAVLEYAFLDMHIPEIVSFTVPDNSRSRGVMERIGMIRDFAGDFLHPK
                     LVSDHRLAKHVLYRIQNSLIKV"
     gene            complement(1812..2600)
                     /locus_tag="I9760_002754"
     CDS             complement(1812..2600)
                     /locus_tag="I9760_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAT5044500.1"
                     /translation="MKSLPIYQVDAFASNLFKGNPAAVCPLDKWLSDETMQAIAAENN
                     LSETAFFVREDEGYRLRWFTPNEEVALCGHATLASSYVLFNQLGFSGKEILFHSLSGK
                     LWVKNTEQGLVMDFPALPYEEIDFRRELEHLNLKKPFKVLQSQFDLMFVYQNAEEVSE
                     AKPDLNAVAHLDYRGLILTAPGTSTDVYSRCFYPGCNVPEDPVTGSAHCVIAPYWCER
                     LKKNHITALQGGLRQGDLYCEVKENRVLLYGNCHLYLEGRIYLP"
     gene            2678..3097
                     /locus_tag="I9760_002755"
     CDS             2678..3097
                     /locus_tag="I9760_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT5044501.1"
                     /translation="MYLSSLLKKTNRLLIKRANELIKPYALNHAYTYILMELYNENGL
                     TQSELVQLIEVEQPTLVRTLDRMERDGFITRKPSSMDRRVVHIFLTEKARLLQKKLDE
                     CSRMLNHEILAGFSEDEKTKLQELTHRVLDNLRNPIE"
     gene            complement(3127..3582)
                     /locus_tag="I9760_002756"
     CDS             complement(3127..3582)
                     /locus_tag="I9760_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HAT5044502.1"
                     /translation="MPVKKLKQFLDSHKIKYLSIAHSPAYTAQEIAASAHVSGKQLAK
                     TVIIKMDGRLAMVVLPASDHITFMKLKEAIGTSDLELATESEFEGKFAECDVGAMPPF
                     GNLYGLPVLVSTKLSAQDNILFNAGSHSELMQLSFGDFEKLVKPTLVTL"
     gene            complement(3742..4314)
                     /locus_tag="I9760_002757"
     CDS             complement(3742..4314)
                     /locus_tag="I9760_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAT5044503.1"
                     /translation="MLLPIQVTFRNMDPSKAVESRAIKLAKKLDCLYDKIMGCRVVIE
                     SPHRHHNKGKLYHVRIDLTVPDAELVVSRESSNKHAHEDVYVSLRDAFNAIERQLKNY
                     ANQRRGHVKRHEDSIRGGRVLEVCPIADYGYIETIDGRLIRFTGQSVVDYDINKLEVG
                     DRVRFVETVKNTQEAAASTVYVEGKRHIMN"
BASE COUNT         1548 a          953 c          736 g         1500 t
ORIGIN      
        1 tataatttac cttttcctta cccttatcag tgactcttaa aaaacgctct ctcttatcca
       61 gcccttcgtt taccgtaacc aattcatcat taatcaaaga attcactatc cgcgacagag
      121 taggtaatga aactaacaac aattgactca atttggaaat agtaatattg ggttctttat
      181 ttatttcaat taaagtatgc caataagaag gctgctcctt tgcagctaac ttatttaatt
      241 gcaacatacc taattctcgt accaattttc ttgaatattg cctgagtaaa tcaattggat
      301 catcaatcat cttgcctctc atactataaa aactatatta ataattgcac atattataaa
      361 ttaatattaa aataattcca tattttatat atttttatta aattacttgg ttagaaagtg
      421 agaaaatcta tttaatgcgg atagaaaacc accctttcca accctgaggt gagatacatt
      481 tagagagttt aagtcataga aataatgtcg tattcactaa atgtaaaacc aaaaaagagc
      541 gtttaaaaat tagctatgat attggctgaa aaacttaata aaaaaacttg gagtttctat
      601 ggaacaatgg catcaattta ctctactatt tcctttaatt attttagttt ttatttcatt
      661 aatcagtcct gggccagatt ttgctatcac agtcaaaaac agcttggctt attccagaaa
      721 gagtggttta ttaaccgcat taggaatagc tttgggcagc ttggttcata tcagttacac
      781 tcttcttgga ttaggcttgt ttattaccaa aacgcctata atactgcata tcatccagta
      841 tctaggcgct ggatacctct tttatattgg ccttaaaaat cttggtgcca agccaaatgc
      901 tgaccaaata ggaagttata caacgaacaa cgatttaaaa ccaagcgaag cttttttcaa
      961 tgggtttata acgaatttat tgaatcccaa agccatgctc ttttttatca gtttattttc
     1021 agtgcttatg cccagagaaa tctcaatctc caccaaatta ttcctggcaa ttgtcctctt
     1081 tctacaaaca ttgttatggt tttcttttgt tgcattcagt ctttctggaa aaaatacgcg
     1141 tacgaaattt caaagaatta gctattggat tgagcgtatt acaggtgtca ttttaattgc
     1201 attggcatta aaattattgc taagccaatt ggtctaatgc tcagatagaa gaacatacaa
     1261 aatccatcaa acttttatta aggaattctg tatacgatat aaaacatgct tcgctaacct
     1321 gtgatcagag actaatttag gatgtaaaaa atctcccgca aaatctctga tcatcccaat
     1381 ccgctccata acaccccttg agcgggaatt atcaggtacg gtaaaagaaa ctatttcggg
     1441 aatatgcata tccagaaaag catattcaag taccgctttt gccccttctg tagcataacc
     1501 ttgcccccaa aactcagttg ccaaacgcca gcctatctct acacaaggag tgaagtgagc
     1561 attccaggtt ggagcattta atccaataaa accaataaac tgcttattgt ccttcacttc
     1621 agctgcccac aaagtaaatt ttttttgaga taattgttca ttcattctga taatgaaatc
     1681 tttaaccata tccatagacc acaattcggg gaaatacttc atgacccgag gatcctgact
     1741 cattctatag aaagggatag tatcttctct atcccaagct cttagcaaaa gccttgaggt
     1801 tgttaaatat ttcatggcaa atatattctt ccttccaaat aaagatggca atttccatag
     1861 agtaacactc tgttttcctt cacttcacaa taaagatcac cttgtctgag acctccttgt
     1921 aaagcagtta tatggttctt ctttaaccgt tcacaccaat aaggagcaat aacacaatga
     1981 gcagatcctg ttaccgggtc ttctggtaca ttacaaccag ggtaaaaaca acgagaataa
     2041 acatcggttg atgttcctgg agcagttaaa atcaaccccc tatagtcaag atgagccaca
     2101 gcattcaaat caggcttggc ctcactcact tcttctgcat tttgatacac aaacatcaaa
     2161 tcaaattgac tttgtagtac cttaaatggt ttttttaaat tcaaatgctc tagttccctt
     2221 ctaaaatcga tttcttcata aggtagagct ggaaaatcca taaccaagcc ttgctctgtg
     2281 tttttaaccc atagtttacc gctcaagcta tgaaataaaa tttcttttcc agaaaatccc
     2341 aattgattaa aaagtacata agagctggca agagtagcat gcccacataa tgccacttct
     2401 tcattggggg taaaccatct gagcctgtat ccctcatctt ctcgaacaaa aaaggctgtt
     2461 tcagataaat tattttcagc agcaatggct tgcatagtct catcacttaa ccatttatct
     2521 aacgggcaaa cagcagcagg attacctttg aataaatttg aggcaaatgc atccacttga
     2581 tagattggta agcttttcat agatgtctcc taaaacaact tgtagataat aacatagctt
     2641 gttattatat agcaagctat ttaattgtga gaaatttatg tatctttcct ctctgctaaa
     2701 aaaaacaaat cgtttgttaa tcaagcgagc caatgaatta attaaacctt atgctctgaa
     2761 tcatgcctac acttatatat tgatggaact gtataatgaa aatgggttga cgcaatctga
     2821 attggttcaa ttgattgaag tggaacaacc tacccttgta agaactttgg atcgaatgga
     2881 aagagatgga tttattactc gaaaaccaag ctccatggat agaagagtcg ttcatatttt
     2941 tcttacagaa aaagccagac ttttgcaaaa aaaattagat gaatgttcta gaatgcttaa
     3001 ccacgagata ctcgctggtt tttcagaaga tgaaaaaaca aaattacaag agcttactca
     3061 tcgcgttctt gataatttaa ggaatcccat tgaataattt ataatgatat tactaatcgc
     3121 ttcattctat agcgtcacca atgtgggctt tactaatttt tcaaaatcac caaaagagag
     3181 ttgcatcaat tccgaatgag aacccgcatt gaataagata ttatcctgtg cactcagttt
     3241 agtcgacacc aatactggta aaccatacaa attaccgaat gggggcatag ctccaacatc
     3301 acattccgca aatttccctt caaactctga ttcagtagca agttcaagat cagaagtacc
     3361 aatagcttct ttaagtttca taaaggtaat atggtcactt gcaggtaata ctaccatggc
     3421 taaacgtcca tccattttta taataaccgt ctttgccagt tgttttcctg aaacatgagc
     3481 agaagcagcg atttcttgtg cagtgtacgc tggtgaatga gctatagaca aatattttat
     3541 cttatggctg tccaaaaatt gtttaagctt tttaactggc atgacaaact ccttttcgat
     3601 actaactcat tctttcagaa aatgccctct ctcaagtgcc cttatttaaa attttgaatc
     3661 atttgtttgg aagcagtcat ttcgcctaaa caattactca acagcaaact gcaaattacc
     3721 aataggcctc tgatacccct gttagttcat aatatggcgt ttaccttcaa cgtaaaccgt
     3781 acttgcagca gcctcctgtg tgtttttaac cgtttcaaca aatcgtactc ggtcaccaac
     3841 ctctaattta ttgatgtcat aatcaacgac actttggcct gtaaatcgta tcagacgccc
     3901 atcaattgtt tctatgtagc cataatcagc aatgggacat acttcaagaa ctctcccacc
     3961 acgaatagaa tcctcatgac gcttgacatg gcctcgccgt tgatttgcat aatttttaag
     4021 ctggcgctca atcgcattaa aagcgtctct tagagaaaca tatacatctt catgcgcatg
     4081 cttgtttgat gattcacggc tcacaactaa ctcagcatca ggaactgtca gatcaatacg
     4141 tacatgataa agctttcctt tgttatgatg gcgatgagga gactcaatga ctactcgaca
     4201 acccattatt ttgtcataaa gacaatcaag ctttttagca agttttatag ccctgctttc
     4261 aacagctttt gatggatcca tatttcggaa tgtcacctga ataggaagta acatttttaa
     4321 cctcccttat gttttgatta tgaataattt cagatcttat ttactaatct atagacaaaa
     4381 aaaatgttgc tttcaataga aaaatgataa gcacttgaat taacaataaa aataaaatta
     4441 ttaaaaccaa actaaaaaat ggtataaaaa atgaattgag ttgacataca aatactttat
     4501 atctgagaaa cttaatgagc atataacaac aggagactga tgctttttgg ggggattgca
     4561 tacgtggata agcctaagag ttcgacgctg cactccgaag caagatcgct tcagaccatt
     4621 ctctttaact tacactcgtc ctcaagtcag ccaccttctc cattcatttt agatttggaa
     4681 cagctgtact ttttgactcg gcagtagact ataaaattac cggattttat gtcaaac
//
LOCUS       DACTJE010000202         4710 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.204, whole genome shotgun sequence.
ACCESSION   DACTJE010000202 DACTJE010000000
VERSION     DACTJE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.204"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1731
                     /locus_tag="I9760_002758"
     CDS             <1..1731
                     /locus_tag="I9760_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5044504.1"
                     /translation="DILEKKVADRTKELEKILALTKSTLESTADGILVVDLKGNIEFC
                     NQQFLKMWGIAPGFIDSHNDTETIQYVLNQLKNPEEFLKQIKELYNNPKQESFDELHF
                     KDGKIFERYSQPHWLDNKIIGRVWSFRDETLRTRLAYQANHDSLTGLPNRTALYDRLD
                     HAINYCKRLNTSLSVLFLDIDNFKLINDSLGHDAGDILLYEFSRRLKGCIRHSDTVAR
                     FGGDEFVLLLMTTEPKDVILIAQNILKAVAQPIKLPTQEVIVTTSIGISLFPKDGIDA
                     STLLKNSDTAMYLAKKEGRNNFQFYNNSINQQSLKRLEIQTQIHNAIKNNEFFILYQP
                     IYNLRSGELVGAEALIRWGHPAIGLLSPDEFIPIAEETGLINQVGEWVLRNACVQNKT
                     WQLMGLPHISISVNVSWRQLKNSNFIEIAESVLKESRLDPQYLEIEFTESTMIQKSTP
                     IDSTLIKISQLGIKMAIDDFGTGYSNLSYLKTFPFDKLKIDRSFIQNCTTNPNDASIV
                     EAIIAMAHSLELKVLAEGVDTIDKAFFMHQHGCDEVQGFLYGLPVKADKFVKLMQKTK
                     IRHSKFINKT"
     gene            complement(1870..3000)
                     /locus_tag="I9760_002759"
     CDS             complement(1870..3000)
                     /locus_tag="I9760_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAT5044505.1"
                     /translation="MRSFLLALLLAINTVVLADELVGFAAFENGDYTTAYPHLMQAAK
                     EGNEEAMYLLGRMYQYGYGVTTNYEEARNWYQKAADKNNALAQLSLGFMYDTGKGVSQ
                     DFTEAFKWYMKAAEQGNPIAQRNIGLMYATGDGVAASDDKAFNWFKKAAEQGYSKAQV
                     NLGYQYMMGKGTPKDVKKAFEWYQKAAEQGDEKGEYSLGLLYTGQEGGIGADDKAAFY
                     WFSQAANHGHVNAQTYLAYYYLKGYGVDADPVKAAYWYQSAAEKGQPEAQAQLGQLLL
                     TGTGVDKDYQQAAYWFGKSAHQGNPIGQAKLGYMYLAGLGVNKSLVKAYAWLKIAAEN
                     KNEEAAKQLKSLEAKLTEPEKLEAEKMIKDLGPLESKENYED"
     gene            3487..>4710
                     /gene="sidG"
                     /locus_tag="I9760_002760"
     CDS             3487..>4710
                     /gene="sidG"
                     /locus_tag="I9760_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidG"
                     /protein_id="HAT5044506.1"
                     /translation="MFLPKGGQKMSRSKDEVLEANDSLFGITVQTWGTNDRPSNGMMN
                     FADQQFFGGDVGHASINMKLPVTDKTKQWIEKYCYSQTYDQFKKVKGNEDKTYEEYLK
                     TAKRLIPVELKTQVTRKAQYDSNGNLVTTHEKAYEQIYFDIDWSWWPGRLQNTEDDMV
                     WEREGKHFEYDEKWKEYLQPEQRVHRGKLGSRKMDYAPTSIIHQRDIPTSELEKITRD
                     HKIHTIEEKLNVVKLLQSKIDEMPHTKMSPSMELMFKNLGINVEKLLDETKDNGVDPT
                     NLEAMREYLTNRLTERKLELETELSEAKKEVDSTQVKNKVEDVYYDFEYKLNQVRKKM
                     EEVNSQLEKMDSLLHKLEGNTSGPIPYTAEIDELMSVLPFLKEELELENGTLSPKSIE
                     NLIDHIDELKNELASK"
BASE COUNT         1625 a          882 c          792 g         1411 t
ORIGIN      
        1 gatattttag aaaaaaaagt agcggacaga accaaagaat tggaaaaaat cttggcttta
       61 accaaatcaa ctttagagtc aacagcggat ggtattttag ttgttgatct caaaggtaat
      121 attgaattct gcaatcagca atttctcaag atgtggggaa ttgctccagg gtttattgac
      181 agtcataatg acacagaaac aattcaatat gtattgaatc agcttaaaaa tccggaagaa
      241 tttttaaaac aaattaaaga actatacaat aatccaaagc aggaaagctt tgatgaattg
      301 catttcaaag acggtaaaat ctttgagaga tactcacaac cacattggtt agacaataaa
      361 attatagggc gtgtttggag ttttagagat gagacattaa gaaccagatt agcttatcaa
      421 gccaatcatg attctctaac tggtttaccc aatcgtacag ctctatacga tcgcctggat
      481 cacgcgatta attactgtaa acgattaaac acctcattat cggtattatt tctggatatt
      541 gataatttta agttaattaa tgacagcctg ggccatgatg ccggtgatat attattgtat
      601 gaattttcca ggcgtcttaa aggatgcata cgtcatagcg atacagttgc tcgatttggc
      661 ggtgatgagt ttgttttgtt attgatgacc actgaaccta aagatgttat tctaatagcg
      721 caaaacattt taaaagctgt agcgcaacct atcaaattgc ctacccagga agtcatagtg
      781 acgacaagta taggaattag ccttttccct aaagatggca ttgatgcaag caccttactc
      841 aaaaattccg ataccgcaat gtacctggct aaaaaggaag ggcgcaataa tttccaattt
      901 tataataatt cgatcaatca acaatcgctc aaacgccttg agatacaaac acagatacac
      961 aatgcaatta aaaataatga attttttatt ctttaccagc ccatttataa tttacgtagc
     1021 ggtgaattag taggtgccga agccctgata cgttgggggc atcctgcaat aggactcctc
     1081 tctccggatg aatttattcc tattgctgaa gaaaccgggt tgattaacca agttggcgag
     1141 tgggtgttaa gaaatgcctg tgttcaaaat aaaacctggc aacttatggg gttaccgcat
     1201 attagtattt ccgttaatgt ctcatggaga cagttaaaaa atagcaattt tatcgagatt
     1261 gcagaatctg tattaaaaga atccagacta gatcctcagt atttggaaat tgaattcacc
     1321 gaaagtacta tgatacaaaa atcaacgccc attgattcaa cgttaataaa aatttcccag
     1381 ttgggcatta aaatggcaat tgatgacttt ggtaccggtt actcaaattt aagttattta
     1441 aagactttcc ccttcgataa acttaaaatt gatcgttcat ttattcaaaa ctgcaccact
     1501 aacccaaatg atgcctccat agttgaagca attattgcca tggcccatag cctggaactc
     1561 aaagtattgg cagaaggagt tgacacgatt gataaagcgt tttttatgca tcaacatggc
     1621 tgtgatgaag tacaaggttt cctttatggc ctacctgtta aagctgataa gtttgtgaaa
     1681 ttaatgcaaa aaactaaaat ccgccattca aaatttatta ataaaacata aggttgttgt
     1741 ttcttttagt actataaaga aaatactaca gactactcat caattgaact agctcattta
     1801 aaccagtatt ttaaacacac aacaaattat caagttacaa ctataatctt ctggattcag
     1861 tttttaactt tagtcctcat aattctcttt gctttctaat gggcccaaat ccttgatcat
     1921 tttctctgct tccaattttt caggttcggt caacttggct tccaaagatt taagttgctt
     1981 tgccgcttcc tcatttttat tttcagcagc aatttttaac caggcgtaag ctttaaccaa
     2041 gctcttgttg actcctaatc ctgctaaata catgtacccc aacttggctt gtccaatagg
     2101 atttccctga tgtgctgact taccaaacca gtaagctgcc tgttggtaat ctttatcaac
     2161 acctgtacca gtcaataaca attgtcctaa ttgtgcctgt gcttcaggtt gccccttttc
     2221 tgctgcagat tgataccaat aagctgcttt cacaggatca gcatctacac catacccttt
     2281 aagatagtag taagctaaat aggtttgtgc gttgacatgt ccatgattgg ctgcttgtga
     2341 aaaccagtaa aaagcggctt tatcatccgc accaatccca ccttcctggc ctgtatacaa
     2401 tagacctaga ctgtattcgc ccttttcatc cccctgctct gccgcttttt gataccattc
     2461 aaatgctttt tttacatctt ttggagttcc tttacccatc atgtattgat aacccaaatt
     2521 cacttgtgcc ttgctatagc cttgttctgc cgccttttta aaccagttga atgctttatc
     2581 atcactggca gctacgccat cccctgtggc atacattaat ccgatatttc tttgtgctat
     2641 cggatttcct tgctctgctg ccttcatata ccatttgaag gcctcagtaa aatcctgaga
     2701 taccccctta cctgtatcgt acataaatcc caaacttaat tgagccaatg cattattttt
     2761 atcagctgcc ttttgatacc aatttctggc ttcttcgtaa ttagttgtca caccatatcc
     2821 gtattgatac atgcgcccca ataaatacat ggcttcctca ttaccttctt tggctgcctg
     2881 cattaaatgt ggataggctg tggtgtagtc gccattttca aatgctgcaa aaccaactaa
     2941 ctcatcggcc aaaacaacgg tgttaatcgc caataataga gctaacaaaa aacttctcat
     3001 acttgctcca ttttattccc ttttaattta agcgtagaat aaagaagcaa caaagcaagt
     3061 aattgatcaa tttaagatat aaaacaaacc agacacataa tccaaaacaa aatatcttaa
     3121 ttttatattt atttcatcga tcaagctaaa acatatttta tagcaatgaa aaatatataa
     3181 gacacagatt ttcttaaaac tatagaaaaa actaataatg tcagagccat cgaacataca
     3241 ctatttcgac ataattcctt atctcgatcc tcctgataaa tctaagcaca tcttaataaa
     3301 tatcaaactt aattagactc aaagctatta atttaagtta aaacataacg acccaaatac
     3361 aaataaaagc gcaaataatt ttatttactc acccaaaaac tcataccaaa aactcttaat
     3421 ataagattaa tttttatgca ctataataat tataagatct aatagtaaaa ataataatct
     3481 aagtgtttgt ttttacccaa aggaggacag aagatgtccc gttcgaaaga tgaagtttta
     3541 gaagcaaatg attctctctt tggtattaca gtccaaacat ggggaactaa tgatcgaccg
     3601 agtaatggaa tgatgaattt tgctgatcag caattttttg gtggggatgt aggtcatgct
     3661 tccattaata tgaaattacc tgtgactgat aagacaaagc aatggataga aaaatattgt
     3721 tattcacaaa catatgatca atttaagaaa gtaaagggta atgaagataa aacctatgaa
     3781 gagtatctga agaccgcaaa acgacttata cctgtagagt taaaaacgca agttacgcgg
     3841 aaagcccaat atgattcaaa tgggaacctt gtaactaccc acgagaaggc ttatgaacaa
     3901 atttattttg acatagattg gagttggtgg ccgggaagat tacagaatac agaagatgac
     3961 atggtttggg aaagagaggg aaagcatttt gaatacgacg aaaaatggaa agaatatcta
     4021 cagcctgaac aaagggtgca taggggtaaa ttaggctcca gaaaaatgga ctatgctcct
     4081 acgtcaatta tacatcaaag ggatatccct accagtgaac tagaaaagat tacacgtgat
     4141 cataaaattc atacaattga agaaaaacta aatgtagtaa aactgttaca aagcaaaatt
     4201 gatgaaatgc ctcatacaaa aatgagccct tcaatggagc taatgtttaa aaacttaggc
     4261 attaatgtcg aaaaattgct tgatgaaacc aaagacaatg gagtagatcc aacaaactta
     4321 gaagcaatgc gagaatattt aacaaaccgc ctgacagaac ggaaattgga attagaaact
     4381 gaactctctg aagctaaaaa agaggtagac tcaacacaag ttaaaaataa agtcgaagat
     4441 gtttattacg attttgaata caagttaaat caagtacgta aaaaaatgga agaagtaaac
     4501 tctcaacttg aaaaaatgga tagtttactt cataagctgg agggtaatac atctggaccc
     4561 attccttata cagctgaaat tgacgaactg atgtctgtct tacctttttt aaaagaagaa
     4621 ttagaactgg aaaatggtac attgtcaccc aagtccattg aaaatttgat cgatcatatt
     4681 gatgaattaa aaaatgagct tgctagcaaa
//
LOCUS       DACTJE010000203         4637 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.205, whole genome shotgun sequence.
ACCESSION   DACTJE010000203 DACTJE010000000
VERSION     DACTJE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.205"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(114..1463)
                     /gene="dsbD"
                     /locus_tag="I9760_002761"
     CDS             complement(114..1463)
                     /gene="dsbD"
                     /locus_tag="I9760_002761"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAT5044507.1"
                     /translation="MRKLIDSFMILLLIFFTQAAWSYTPGFESSNPDTVMHFITGNSA
                     FVYLSAFFGLGILLAFTPCVLPMVPILSGIIVGQNSISTGKAFKLSLSYVTGMAITYA
                     GAGMLAGYMGSTIQTVMQRPVVITLFSVVFVAMALSMFGFFELKFPTSLGNRLNKNNQ
                     LGKQRSYLSVGIMGVLSTLIVSPCVTAPLIGVLSYIGQNGQVIMGGLILFVMALGMGV
                     PLLIVGAGYGTLLPKAGSWMLKIKQLFGFIMLGIAIWMLSRILNQTITHILWAGLLMV
                     ASISLGALKSQNNWRGYLAQGLGILALISGGIIFYNTAIPSVKIKQASVENSPFIKIN
                     TLNALQLQLEQAKKVRKAVFLDFSADWCGDCQEMDTKVFNQPEIQKAMSDWVSIKVDI
                     SDKNAEVDSIKKAFGIYGTPTMIFFNTSGEPITQMTSVGLISLSKMLQLLEQARHLS"
     gene            complement(1591..1908)
                     /locus_tag="I9760_002762"
     CDS             complement(1591..1908)
                     /locus_tag="I9760_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044508.1"
                     /translation="MKLKYRVIHYLILCSVVLLMIKPAVFHNNNQQTQSNTQYIHSIT
                     ECPQRTALMNYCSSSAYYIISEEELSLGNVLSLLTFILYFLVIQSKYKSPVSPIFKPP
                     IAH"
     gene            complement(2005..2856)
                     /locus_tag="I9760_002763"
     CDS             complement(2005..2856)
                     /locus_tag="I9760_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAT5044509.1"
                     /translation="MAMNTKVILITGCSSGIGFDAVFALKNRGHRVIASCRKTEDVQK
                     LLNKGVEALLLDVSNPISIQSAFAQLLIMTSGHLDVLINNAGYGQVGALEDISRDILR
                     QQFETNVFGLQDLTNLVIPVMREQGQGRIINISSILGVVSMPFRGAYNASKYALEGLS
                     DTLRLELSGSGINVITIEPGPINSRFRDNAVDLSLQQIPMEKSYFKNQYRNMLSNFKQ
                     KKADSFFTRDTDAVIKKLVHAVESRKPKPKYPVTFPAYFLIGLKRFFTTRLLDRFILF
                     LSRKELG"
     gene            3010..4518
                     /gene="ravZ"
                     /locus_tag="I9760_002764"
     CDS             3010..4518
                     /gene="ravZ"
                     /locus_tag="I9760_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavZ"
                     /protein_id="HAT5044510.1"
                     /translation="MKGKLTGKDKLIVDEFEELGEQESDIDEFDLLEGDEKLPGDSEL
                     DKTTSIYPPETSWEVNKGMNSSRLHKLYSLFFDKSSAFYLGDDVSVLEDKPLTGAYGF
                     QSKKNDQQIFLFRPDSDYVAGYHVDAKSDAGWVNDKLDRRLSEISEFCSKATQPATFI
                     LPFVEMPTDITKGVQHQVLLTISYDPKSKQLTPTVYDSIGRDTYSESLSSYFKGKYRT
                     TCDEILTQSIEKAIKSTDFTLGKFTRAAYNHQNRLTEGNCGSYTFRTIKEVISSSAQG
                     TEVKIPGSGYITSNSYLTSQHVQDIESCIKYRNLGVVDIESALTEGKTLPVQLSEFIV
                     ALEDYGKLRSQQSEKSMLNFIGYSKTAKLTAVELLIGILNDIKGKNEISESQYDKLVK
                     EVDCLMDSSLGKLVQFHLKNLGAESLQKLVLPCVKFDDTIDDFVTIEKDELFDVPDIT
                     GEELASKKGIEQGALDKEALLKQKQIKTDLLDLREEDKTGLKKPLHGGIKVK"
BASE COUNT         1567 a          874 c          805 g         1391 t
ORIGIN      
        1 tcaaattcat gcttttcaat attttgggta cagtttgaat ttgatttatt tatcaaatat
       61 taaccatacc caaattatta agaacaaatt tcggtagcaa attgtctatt atttcaactc
      121 aaatggcgcg cttgctcgag caattgaagc atttttgata gcgaaatcaa accaaccgaa
      181 gtcatttgag taataggctc tcccgatgta ttaaaaaata tcatcgttgg cgttccataa
      241 ataccaaaag ctttttttat agaatcaacc tctgcattct tatcactgat atccacttta
      301 atgctaaccc aatcagacat agctttttga atttctggtt gattgaatac tttggtatcc
      361 atttcctgac agtcaccgca ccaatccgct gagaaatcaa gaaatactgc ttttctaact
      421 tttttagcct gctccaattg cagttgaaga gcgtttaagg tattaatctt gataaatggg
      481 ctattttcta cagacgcttg ttttattttt actgaaggta ttgcggtgtt gtaaaaaata
      541 atacctcctg aaatcaaagc aagaataccc aatccctgag ccaaataacc acgccaattg
      601 ttttgtgatt ttaaagcacc taaactgatg ctggcgacca tgagtaatcc tgcccacaaa
      661 atgtgcgtaa tggtctgatt cagaatccgg gataacatcc atatggcaat tcccaacatg
      721 ataaaaccaa acaattgttt tattttaagc atccaggaac ctgcttttgg gagtaaagta
      781 ccataacccg caccaacaat cagcagagga acccccatcc ccaatgccat aacaaataaa
      841 atcaatccgc ccattatgac ctggccattt tgaccaatat aacttaaaac accaattaaa
      901 ggagcggtaa cacaaggaga aacaattaaa gtggataaaa ctcccattat tccaacagaa
      961 agataacttc tctgcttccc taattgattg tttttattta accgattccc aaggcttgta
     1021 ggaaacttca attcaaaaaa gccaaacatt gaaagagcca tggctacaaa cacaacactg
     1081 aataaagtga ttactactgg acgttgcatt acagtctgga ttgtacttcc catatacccg
     1141 gcaagcatcc ctgctcctgc ataggtgata gccattccag tcacataact cagtgataat
     1201 ttaaaggctt ttcctgtaga aatagaattt tgtcctacaa taattcctga taaaatagga
     1261 accatcggta gaacacaggg cgtaaaagcc aacaaaatac ctaatccgaa aaaagcactc
     1321 aaataaacaa aagcactatt tcctgtaata aaatgcatca cagtatccgg gttagaactt
     1381 tcaaagccag gagtatatga ccacgccgcc tgagtaaaaa atatcagtaa taaaatcatg
     1441 aacgaatcaa ttaattttct cattttaatg tcccaataag taataaataa caacgataga
     1501 tacttgtatc agagtacaag taacctacac tggacctctt gcatagccag ggctggcaag
     1561 aagcctactg agaaagttga caaatttact ctagtgggca atcggtggtt taaaaattgg
     1621 ggatacaggc gatttgtatt ttgattgaat aactaaaaaa taaaggataa atgtcaacaa
     1681 ggataagaca ttaccgagag ataactcctc ttcagaaata atataatagg cactggacga
     1741 gcaataattc atcaatgccg tcctctgagg gcattccgtt atagaatgta tatattgggt
     1801 attactctga gtctgctgat tattgttatg aaacactgct ggttttatca ttaacaaaac
     1861 cacgctgcat agtatcaggt aatgaattac tctgtattta agtttcataa taactatagt
     1921 atctttttaa gagaggtctg tacagtagtt gctaattatc aacatcaact tatgactatt
     1981 ttataatatt accaataagt tgctttaacc cagctccttc ctggataaaa ataaaataaa
     2041 cctatctaat aaacgtgtag taaagaatcg ttttaaacca attaaaaaat aagctggaaa
     2101 agttacaggg tacttgggtt ttggtttcct tgactccacg gcatgaacca gtttttttat
     2161 cactgcatca gtatcccgag tgaaaaacga gtctgccttc ttctgtttaa aattgctcag
     2221 catatttctg tattgattct tgaaataact tttttccatt ggtatttgtt gtaaagataa
     2281 gtccaccgca ttatcacgaa atcggctatt tatagggcca ggctctattg ttatgacatt
     2341 aatacctgaa ccagacaatt ccagtcgaag tgtgtcactt aagccctcca atgcatattt
     2401 ggaggcatta taagcaccgc gaaatggcat gctaactacg ccaagtatag agctgatatt
     2461 gattatgcgt ccttgtcctt gttccctcat tacaggaata accaaattgg tcaaatcttg
     2521 caaaccaaat acgttcgttt caaattgttg gcgaagaata tcccgagaaa tatcctcaag
     2581 agcacctacc tgaccgtatc ctgcattatt aatcaataca tccaggtgac ccgacgtcat
     2641 aattaacaac tgcgcaaatg cactttggat tgaaataggg ttacttacat ccaacaagag
     2701 agcctcaacc cctttattta gtaatttttg gacatcctct gtttttctgc aggatgcaat
     2761 cactctatgt cctctatttt tcaaagcaaa aacagcatca aaacctatcc cactggagca
     2821 tcctgtgatc aaaataactt ttgtattcat cgccatgacc tatgcctaat ttttaactat
     2881 cgtactaagc tatctatcga attaccacta ttatgataat tatttgttca atttttgact
     2941 tatatttcac tttttcctaa aaatcatttg ctaaactttt aatatggaaa tgacagttta
     3001 gaggtcaaaa tgaaaggcaa gttaacaggt aaagacaaat taatagtgga tgagtttgaa
     3061 gaactgggag aacaggaatc cgatatcgat gaatttgatc ttcttgaagg tgatgagaaa
     3121 ttgcctggcg actcagagct tgacaaaact acatctattt atcctcccga aaccagctgg
     3181 gaagtgaata aagggatgaa cagttctcgt ttgcataaac tatacagttt attctttgac
     3241 aaaagttccg ccttttatct tggcgatgat gttagtgtat tggaagacaa gcctttaaca
     3301 ggtgcctatg gttttcagag taaaaaaaat gatcaacaaa tttttttatt cagaccggat
     3361 tctgattatg ttgcaggata tcacgttgat gcaaaatcag acgctggatg ggtaaatgac
     3421 aaactcgatc gtcgtttatc tgagattagt gaattttgta gtaaggccac tcaacctgca
     3481 acgtttatac ttccttttgt tgaaatgccc actgacatta ccaaaggggt tcaacaccag
     3541 gtattgttaa ctattagcta cgatccaaaa agtaaacaac tcactcccac tgtctatgac
     3601 tccattggac gagataccta ctctgaatcg ttatcatcct attttaaagg caaatataga
     3661 acaacttgcg atgaaatttt gacccaaagt attgaaaaag ctattaagag cactgatttt
     3721 actcttggta aatttacacg agctgcttat aaccatcaaa accgcctcac cgaaggcaat
     3781 tgcgggtctt ataccttcag gacgattaaa gaggttattt catcctcagc acaaggtact
     3841 gaagtaaaaa ttcctggctc aggatatatt acttctaaca gttatttaac aagtcagcac
     3901 gtacaagaca ttgaaagttg cattaaatat agaaatttgg gggttgttga tatcgaaagt
     3961 gctttgactg aaggtaaaac attaccggta cagctttctg aatttattgt tgccttggaa
     4021 gattatggga aattacgaag tcaacaatct gaaaaaagca tgttgaattt tattggctac
     4081 agcaagacag caaaattgac cgctgttgag ctgctaatag gcattttaaa tgatatcaaa
     4141 ggcaaaaatg aaatcagtga atctcaatat gataaattag taaaggaagt cgattgttta
     4201 atggattcat ctttaggaaa gttggtacaa tttcacctta aaaatttagg cgctgaatca
     4261 ctacaaaaac tggtacttcc gtgtgtcaag tttgatgata cgatagatga ttttgtgact
     4321 atcgaaaaag acgaactatt cgatgtaccc gatattacag gggaagagct tgcctctaaa
     4381 aaagggattg agcaaggtgc tctggataaa gaggctttac taaaacaaaa acagattaaa
     4441 acggatttgt tagaccttcg cgaagaagat aaaaccggtc tgaaaaaacc attgcatggt
     4501 ggcattaagg taaaatagtg tgaattgaat tcaactatgt gattaatagg cagaaggttt
     4561 tttaatgcgc tgataatttc ttaaacttta aaaaataagc gagccatgct gtcatcaatt
     4621 attgaattta agttgaa
//
LOCUS       DACTJE010000204         4602 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.206, whole genome shotgun sequence.
ACCESSION   DACTJE010000204 DACTJE010000000
VERSION     DACTJE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.206"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            285..1562
                     /gene="legC7"
                     /locus_tag="I9760_002765"
     CDS             285..1562
                     /gene="legC7"
                     /locus_tag="I9760_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC7/YlfA"
                     /protein_id="HAT5044511.1"
                     /translation="MATNETELQVLIQHDSKSTITTSSLDSTDKDPKASTGTPEIESA
                     SLAQIVDTQKQLSQVKESLESIVDSIAENPSLITRAASAWGELPMWQKVTGGLVLTAP
                     TLAVGLFAHIGVLLVIGGVTGLTYTAGAIVLDDHHTCNVNIAKRLKEGLFGLADLLQI
                     TIEALDAIRKKFAEEIEKFKNENLRLTDNIDRLGNEVESLSAQVELYMEIEKMLRKDT
                     NEMEQTVKKLQESTTKQTDLLEKNQKELSKIRKEYEKSQLQLAEKIAELHEVRLSLGL
                     EVQKAKTVAKTLEGTVQTLTGTVIADEEQRVSFQKKLDGFLNDKQLSFDQVAERICKA
                     EEELKKVKEELRQSNDRYSELLKRQEQQVERLEKLGLHKLERIVDKENVKPSNDPVHS
                     GLLSHGIYSTPKGKVTQPKVEVVEDRQTIALVN"
     gene            complement(1925..3916)
                     /locus_tag="I9760_002766"
     CDS             complement(1925..3916)
                     /locus_tag="I9760_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HAT5044512.1"
                     /translation="MEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKA
                     IVTRREPSLDAVFVEYGSKRQGFLPLKEIAPEYLSKNPEEFGDEKPPITSLIREGQEL
                     LIQVDKEERGNKGAALTTFITLAGCYLVLMPNNPHSGGISRRIEGDERDELRETLNAL
                     TLPEDMGLIIRTAGVGKSLEELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIR
                     SIRDNLRKSISEIIIDDQISYIKAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQI
                     ETAYQRQVMLPSGGALVIDRTEALVSIDINSAKATGGADIEATALNTNLEAADEIARQ
                     LRLRDLGGLVVIDFIDMSSSKNQREVENRLKEALQADRARIQVGRISRFGLLEMSRQR
                     LRLSLGETAQEICPRCEGRGTVRSIQSHGLSITRLIEEEALKEKTAEIQVQLPPEMAT
                     FIMNEKRNFIRDIEKRHGVSVVVIANPYMHPPQYTITRLKEDNVGKSKKPSYSLIQQP
                     EVHVPSSDKEPVSHDEPAVKSFAEHRVIKHPQTSFIKRLWTSLFGGHSVAHTVTQEPG
                     KKHHRAQNQGKHASSKSSGGDKRQQGSNANRRRRSGGGQQRSSSGNTNAAASTHQQRK
                     KSNANQQSHGSQQSNRSIPAKNEGNKKREAILAKEPEEK"
BASE COUNT         1373 a          838 c          952 g         1439 t
ORIGIN      
        1 attgttttat ttgcatgtga aggcaaattt ttatttttaa ataaaagaaa agattatttt
       61 gtcatctgat attgcatgaa gaatattaat tcataaagaa cattaataag tcaaaagatg
      121 ataatgcctc attttgactt agtatttgaa ttttcagatc ttttttgtag taggtttatt
      181 aaaataaaat ttaattgcaa aaaatgcaag aattacttgt cttattgata atttatgatt
      241 aaaaatggac atctaaggaa gatttttaat cgtaaggagt aaatatggct actaatgaaa
      301 cagagcttca agtattgatc caacatgata gcaaatcaac aatcactact tctagcttgg
      361 acagtactga taaggatcct aaagcaagta ctggcactcc tgaaattgaa agcgctagtc
      421 ttgcccaaat cgtcgatacc caaaaacaac tgagccaagt taaagaaagc ctggaatcaa
      481 tagtagacag tatagcggaa aatcctagcc tgattactcg cgctgcgtca gcctggggcg
      541 aactgcctat gtggcagaaa gtcacgggtg gccttgtcct gacagcgcct actttggcag
      601 taggattatt tgcgcacatt ggcgtcttat tagtaatagg gggagttaca ggactaacct
      661 ataccgcagg tgcaattgtt ttggacgacc accatacatg caatgtgaat attgccaaac
      721 gattaaaaga agggcttttt ggtttggcag atctgttaca aattacgatt gaggcattgg
      781 atgcaattcg caaaaaattt gctgaagaaa ttgagaaatt taagaatgaa aacctcagat
      841 taactgataa cattgacagg ctgggcaatg aagtggaatc actgagtgct caggttgaat
      901 tatatatgga aattgagaaa atgctccgta aagatacaaa tgaaatggag caaaccgtca
      961 aaaaacttca agaatctaca acaaaacaaa ctgatttatt ggaaaaaaat caaaaggaat
     1021 tgtcaaaaat tagaaaggaa tacgaaaaaa gccaattgca gttagccgaa aaaatagctg
     1081 aattacatga agtcagactg tcgttgggtc tcgaagttca aaaagctaaa acagttgcga
     1141 aaacgttgga aggtacagtc cagactttaa ctggaacggt cattgctgat gaagagcaac
     1201 gtgtttcttt tcaaaagaaa ctggatggat tcctgaatga caaacagctc agctttgatc
     1261 aagtagctga acgaatttgc aaggcagaag aagaacttaa aaaagtcaaa gaagaattaa
     1321 gacaaagcaa tgatcgttat agtgaactgt taaagcgtca ggaacaacag gttgaacgtt
     1381 tggaaaaatt gggcttgcat aaattggaaa gaatagtaga caaagagaat gtgaaaccca
     1441 gtaatgaccc tgtccactca ggtttgcttt cccatggaat ctatagtacg cccaagggta
     1501 aagtcaccca gcctaaagta gaggtagtag aagatagaca aactattgct ttagtcaatt
     1561 aagagaagga tttaaggaag aagaatcagg ctgttttaaa ggttataatt ttaagtaacc
     1621 ggttaaggga tatgcaatca aagaatatta agtcctctgt ctttcccgcg aaggcgggaa
     1681 tccatctaaa aattaaccta tatactagct ttatggatag attcccgcct tcgcgggaaa
     1741 gacgactcaa atgatcaggc ttataaacag aagaatttgc atatcccgtg aatagttaca
     1801 attttaaaat cggtatcctg acaagaaaga gtgctgtgtt ttacccgcac aatccagagt
     1861 taactggact gtgcggataa gtgcttattg aaaaggtgaa taggcaaata attgatatgg
     1921 cggcctattt ctcctctggc tctttagcca gtatggcttc cctttttttg ttgccttcat
     1981 tttttgcagg aattgaacga ttgctttgtt ggctgccatg actttgctga tttgcgttag
     2041 atttttttcg ttgttgatgt gtgctggccg cagcatttgt atttcctgat gaagaacgct
     2101 gctggcctcc tcctgaacga cgtctacgat ttgcgttgct gccttgttga cgcttgtctc
     2161 ctcctgagct tttggacgat gcgtgtttac cttgattttg agcacgatga tgttttttac
     2221 cgggttcttg agttacggtg tgggcaactg aatgaccacc aaataaacta gtccataatc
     2281 gtttaataaa actggtttga gggtgtttga tgaccctgtg ttcggcaaat gatttcacag
     2341 caggttcatc atgtgacacg ggttctttat cactgctggg aacatgtact tcaggttgct
     2401 gaatcaaaga gtaacttggt tttttagatt tgcctacgtt gtcttctttc aggcgagtaa
     2461 tcgtatattg tggagggtgc atataaggat tcgcaataac aacaacactg acaccatgcc
     2521 ttttttcaat gtccctgata aaattacgtt tttcattcat aatgaatgta gccatttctg
     2581 gtggcaattg cacttgaatt tcagctgttt tttccttgag cgcctcttct tcgataaggc
     2641 gggtaattga taagccatga gactgtatac tgcgtacagt tcctctacct tcacaacgtg
     2701 gacaaatttc ctgggcagtt tctcctaatg ataatcggag gcgttgcctt gacatttcaa
     2761 gaaggccaaa gcgtgaaatt cgccctactt gaattctggc cctatcagcc tgcagtgctt
     2821 ctttgagtcg attttctacc tctctttgat ttttactaga actcatgtca ataaaatcaa
     2881 taactaccaa accacctaaa tcacgcaggc gaagctggcg agcaatttca tcagctgctt
     2941 caaggttggt atttaatgca gtcgcttcaa tgtcggcacc gcctgtagcc ttggctgagt
     3001 taatatcaat tgataccaga gcttcagttc tatctatgac caaggcacct ccagaaggaa
     3061 gcatgacctg gcgctgataa gctgtctcaa tttggctttc aatctgatag aaattaaaaa
     3121 gagggatgct gctgttatag agttttagat taggtaaaaa ttctggtttt acgcgttcaa
     3181 tgtattgttt tgctttgata taggatattt gatcatcaat aataatttca ctgatggatt
     3241 ttcgcaaatt gtcacgaatg gaacgaataa tgacatcgcc ctcttgatga atcaggcaag
     3301 gtgccagttc tgaattataa gcttccttga tggattgcca ctgattgcaa agcatgttta
     3361 agtcatcttg cagttcttcc agactttttc caacacctgc agtgcgaata ataaggccca
     3421 tgtcttcagg gagtgttaaa gcatttaatg tttcgcgtaa ttcatcacgc tcatcacctt
     3481 caatacgtct tgatattccg cccgaatgag ggttattggg cattaaaacc aggtagcaac
     3541 ctgccagggt aataaaagtt gtcagtgctg ctcctttatt ccctctttct tctttatcca
     3601 cttggataag taattcttga ccttcacgga tgaggctggt aattggaggt ttctcatctc
     3661 caaactcttc cggattcttg cttaagtatt caggcgctat ttcttttagg ggtaagaaac
     3721 cttggcgttt ggaaccatat tcaacgaaaa cagcatcaag gctgggttct cttcttgtta
     3781 caatggcttt ataaatatta ccttttttct ttatttctcc cgggcattca atgtccagat
     3841 caaataattg attgttttta attagtgcaa cacgaatttc ttctgtttgc attgcattga
     3901 ttagcatttt ttccatgtat aaccccatat ttagggcgca tgcatttaca tcgagatagt
     3961 gtaaggctca gtatagccgt atgatcattt ctacagtttt cttgccatga agttaatttt
     4021 ccgggtccca tatcttgcat tcgtcttgaa tctgcagaga gagggagtca gaaaattaat
     4081 atcctggtct ctataattct ggtgaaatga taacagccta ttccctaatt gctcaatttt
     4141 gtttttgaaa aacaatgtta ttaagctggc agggtgagga actttaagct gacccatgac
     4201 cttgtggaca gaccgtacgc atgttacctc agtagttaaa ggtgcacact gtatgatgta
     4261 ccgtttacag attaagtggg ttggaaaaaa aaggcctttc atcaggcgtg caaaagcccg
     4321 gcaataattc gtaattggta taaagcatgt ctggcatgca taggtatctg tcgaaataat
     4381 tatcaatttt actctggtat tgggtcgctt ccttatatct tcgacttaaa tggaagcgca
     4441 ttttgttttt tcttccaagc atctactcag gcgtattacg atgaaaaaac agaggtgatt
     4501 ctttaacttg tggttcaagt tcttattgaa ttcaacctgt tttatattcg cttgagcagc
     4561 tgcttcttta gtttgatttg ctatcagatt aactaaattt at
//
LOCUS       DACTJE010000205         4548 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.207, whole genome shotgun sequence.
ACCESSION   DACTJE010000205 DACTJE010000000
VERSION     DACTJE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.207"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..2184)
                     /locus_tag="I9760_002767"
     CDS             complement(<1..2184)
                     /locus_tag="I9760_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT5044513.1"
                     /translation="MCKGYNIMQSMVFHKSSNSYYTAYYLTVAFATLTFIFGLSVLIG
                     WHFNIEFLIQYQPNTVAIVYNTALCFIILSFSIFLFLNCYYKSAITLSTTVFILSGLA
                     LTQHVFSINLQIDEVFFHHYQSIENAYPGRMASNTAFCFLLITTALVLMNIRGYQSFN
                     NSLAGALGLLVFCLALLFISGYFSDIQQAYKWGNKTPMSINTGIGFLLLSLAVMGLYW
                     HNNTLYQLNSGIAMPYLSAFCIMFTFSLLSFEIFKKEKELNLSTNLSISTLALGVFFS
                     ILFGLIIRLWQLAKISAVTAKYALSEIKATLESTADGILVVDRAGKVMNYNKRFLNMW
                     YQEERSLKTINYHDIKLIINKQLINRRETIQRINKILKNPNYQHAFELKFKGEIYYDC
                     YTQPLKLDDEIIGRVWSFRDITIPKRLEIELSHQSTHDMLTNLPNKVLAINMLGYAMK
                     TAVSSKKIVGVYLLDLDRFTQINDVFGHSKGDEIIKAISKRLIDCVPMNHMLGRLSGD
                     QFIVIASINNSKEVVTGVTRLLSAFHEPVKMNGHSIKMSCSVEISFYPKDGNTVDTLL
                     SKADIALSRTKVEGRNSFQFYTQEMHSYTLEHILLESELKNAVAKNEFVLYYQPILKL
                     SSLNVIGFEALIRWNHPRNGLILPDKFIPLAEEIGIIGEIGDWVFVTVCKQIQAWQLQ
                     GFKNIKISINISPKQFKFQYIPKRINELLSEFQVKSESIELELTEN"
     gene            complement(2271..3407)
                     /locus_tag="I9760_002768"
     CDS             complement(2271..3407)
                     /locus_tag="I9760_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HAT5044514.1"
                     /translation="MKKTHSSKTFTHIILWTSLLFFIVAIIWANYAILDEVTTGQGKV
                     IPSSEVQVIQNLEGGIIQNIFVKEGQIVKKDQILMQIDNTRFMASYAEAEKKIDALEI
                     EIIRLNAEISDTKPVFPDKFIKTYPHLVQDQLSLYESRMRELNQLKKSLELAQKELDL
                     TRPLLKGGSVSEVEVIRLERSVSEIKGNIEKFKSEELDKLNKARTELFALIEANKADK
                     DRLTRTTVRSPVYGIVKQIKTTTIGGVVQPGSDLLEIVPLDDTLLIEAKIRPSDIGFI
                     HPGQKAMVKITAYDFSIYGGLEGVVEHISADTIIDEKTDKKEESYYIVKVRTDKNYLG
                     TEKKPLPIIPGMQATVDILTGQKSVLQYLLKPIIKAKQSALRER"
     gene            complement(3411..>4548)
                     /locus_tag="I9760_002769"
     CDS             complement(3411..>4548)
                     /locus_tag="I9760_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT5044515.1"
                     /translation="EKQATLFESLATIETVKTTGAESVLQSRWERIINESSKNSMQLR
                     FVANTSISITNLAQQIATIAIIIAGVYMISEGELTTGGLIACTILTGRALAPMAQVST
                     LFTRYFQSVNALDSLNKIMQLETDTTEETRYLHRPSLKGAVEFKQVSFHYKEDETKVL
                     QNLNFKINAGERVAIIGRVGSGKSTLGKLILKLYVPNEGTILLDGTDYKQINPDDLRQ
                     QIGYVPQDIVLFYGSIRDNICVGAPFINDSALLEAAEIAGVTSFTNNHPKGFDRQVGE
                     KGAELSGGQRQSVALARALIMSPNILLLDEPTSAMDDNNEKQIKKNLNEYLSDNRTLI
                     LITHKISMLELVNRIIVLENGKIIADGNKEAVLSALRTGVTVRK"
BASE COUNT         1452 a          847 c          725 g         1524 t
ORIGIN      
        1 attttcagtt aattctaatt caatagattc tgattttact tgaaattcag ataataattc
       61 attaatcctc ttgggaatat attgaaattt aaactgcttt ggtgaaatgt tgattgaaat
      121 ttttatattt ttaaatcctt gaagttgcca tgcttgtatc tgtttgcata cagtgacgaa
      181 cacccaatca ccaatttcac caattattcc tatttcttct gcaagaggaa taaatttgtc
      241 cggaagaatt aatccattgc gagggtgatt ccagcgaatt agagcttcaa aaccaatcac
      301 attcaaagaa ctcaatttga gaataggttg gtaatacaaa acaaactcat tttttgccac
      361 tgcattcttt aactcacttt ccagtagaat gtgttctaaa gtatagctat gcatttcttg
      421 tgtataaaat tgaaaactat tgcgaccttc aacttttgtt cgagacaggg caatgtcagc
      481 cttagacaat aaggtgtcaa ctgtatttcc atctttaggg taaaacgaaa tctccacact
      541 gcaactcatt ttaatactat gaccgttcat tttaacaggt tcgtgaaatg cactcagtaa
      601 acgtgtgact ccagtaacta cctccttact attgttaata ctggcaatta caataaactg
      661 atcgccacta agtcgtccaa gcatatgatt catcggcacg caatcaatta accgttttga
      721 aatagctttt attatttcat ctcccttact gtgcccaaac acatcgttga tttgtgtaaa
      781 tcgatctaaa tctaataaat atactcctac tattttttta ctactaacag ctgttttcat
      841 tgcatatccc agcatgttaa tagccaatac tttattaggt aaattggtaa gcatgtcatg
      901 tgtagattgg tgagatagct ctatttcaag ccttttagga atagtaatgt ctctaaaact
      961 ccaaacacgc ccaatgattt catcatccag ttttagtggt tgagtatagc aatcgtaata
     1021 tatttcacct ttaaatttta actcaaatgc gtgttgataa tttgggtttt tcaagatctt
     1081 attaattctt tggattgttt cccttcgatt gattaattgt ttattaatta tcaattttat
     1141 atcatgataa tttatcgttt tcagagatct ttcttcttga taccacatat ttaaaaagcg
     1201 tttattgtaa ttcatcacct tgcctgctct atccactacc aaaataccat ctgctgtaga
     1261 ttccagagtt gccttaattt ctgataatgc gtatttagca gttacggctg atatttttgc
     1321 caattgccat aatctaatga ttaatccaaa caggatggaa aaaaaaactc ctaaagcaag
     1381 cgtactgatt gataaattag tgcttaaatt tagttctttt tcctttttaa aaatttcaaa
     1441 tgataacaag gaaaaagtaa acataatgca aaatgcactt aaataaggca ttgcaatacc
     1501 agagtttaat tgataaaggg tattattgtg ccaatataat cccatcaccg ctaaagaaag
     1561 taacaaaaaa ccaatccctg tgttgattga catgggggtt ttattacccc atttataagc
     1621 ctgttgaatg tctgaaaaat aaccacttat gaacaataaa gccaaacaaa atactaataa
     1681 gccaagtgca cctgccagag aattattaaa ggattgatag cctcttatat tcatcaaaac
     1741 aagtgctgta gtgattaata aaaagcaaaa ggctgtatta gaggccattc tgcctggata
     1801 agcattttca atagattgat agtgatgaaa gaatacttcg tcgatctgaa ggttaatact
     1861 aaaaacatgc tgtgtaagcg ctaaaccaga taaaatgaaa acagtagtac ttaaagtaat
     1921 ggctgacttg tagtagcaat tgaggaataa aaaaatagaa aaggataaaa taataaagca
     1981 taaagctgta ttgtaaacga ttgctaccgt atttggttgg tattgaatta aaaactcaat
     2041 attaaaatgc caaccaatta aaacagacag cccaaagata aatgttagag tggcgaaagc
     2101 tacagttaga tagtatgcag tgtagtaaga attggatgat ttatggaaca ccatcgattg
     2161 cataatatta tatcctttgc acagatagcc ttacagaaaa taatattaat attataatag
     2221 accaattaac taattatcgc ataatataac aagttaagga cttctaataa tcaacgttct
     2281 cttagtgctg attgcttagc tttaatgata ggtttcaaga ggtattgaag aacagatttt
     2341 tgacctgtaa gtatatcaac cgtggcttgc atgccaggaa tgataggcaa tggctttttt
     2401 tcagttccca gataattttt atctgttcgc actttcacta tatagtagct ttcttctttt
     2461 ttatctgttt tctcatcaat aatagtatcg gcactaatat gctctacaac accctccaat
     2521 ccaccataga tagaaaagtc ataagcagta attttaacca tcgctttttg gcctgggtgt
     2581 ataaaaccaa tatcagaagg gcgtattttt gcttcaatta ataaagtatc atcaagcggg
     2641 actatctcaa gtaaatcact acctggttga actacaccac caatcgttgt cgtcttaatt
     2701 tgttttacaa taccataaac tggagaacga actgtggtcc ttgttaaacg atctttatcc
     2761 gctttattgg cctcaatcag cgcaaacaac tctgttctgg ctttattcaa tttatccagt
     2821 tcttctgatt tgaacttctc aatgtttcct ttaatttcac ttactgatct ttccagacga
     2881 ataacctcta cttctgaaac ggatccaccc tttaataatg gacgagttaa atccaattct
     2941 ttttgcgcca actctaggga ttttttcagt tgattcaact ctctcatccg tgattcatac
     3001 agagacaact gatcctgcac aagatgagga taggttttga taaatttatc tggaaacaca
     3061 ggttttgtat cactaatttc agcattaagc cttataattt ctatttccaa tgcatcaatt
     3121 tttttctctg cttcggcata gctggccata aaccgagtat tatcgatttg cattaaaatt
     3181 tgatcttttt taactatttg cccttctttg acaaaaatat tctgaataat cccgccttca
     3241 agattctgta tgacctgaac ctcactagag ggaattactt ttccttgtcc tgtagttact
     3301 tcatcaagaa tggcataatt agcccaaata attgctacaa taaaaaaaag caaggaagtc
     3361 cataagataa tatgggtaaa ggtttttgag ctgtgagttt ttttcataga ctatttcctc
     3421 accgttaccc ctgtcctaag ggcgcttaga acagcttctt tattaccatc agcaatgatt
     3481 ttgccatttt ccaaaacgat aatacgattg accagctcca gcatggaaat cttatgagtt
     3541 attaaaatca aagtacgatt atcggataag tattcattaa gatttttctt gatttgcttt
     3601 tcgttattat catccattgc cgaagtaggc tcatccaata acaagatatt agggctcata
     3661 atcaaagcac gggcaagagc gacagattgt ctttgtcctc ctgataattc agctcctttt
     3721 tctcctactt gtctatcaaa gcctttggga tgattattgg taaaactcgt taccccagct
     3781 atttcagcgg cttctaaaag agcagagtcg tttataaagg gggcacctac acaaatgtta
     3841 tctcttatgc ttccataaaa taaaacaata tcctgaggaa cataacctat ttgctgacgt
     3901 aaatcgtcag gattaatttg cttataatcg gtgccatcca gcaaaatagt tccttcgtta
     3961 ggaacgtaaa gctttaatat tagttttccc aaagtcgatt tacctgaacc aactcgtcct
     4021 ataattgcaa cgcgctcacc agcattaatt ttaaaattaa gattttgtaa taccttcgtc
     4081 tcatcttcct tgtaatgaaa agacacctgt ttaaattcaa cggcaccttt cagagaaggt
     4141 ctgtggagat aacgagtttc ttctgtggta tctgtctcaa gttgcataat cttgtttaat
     4201 gagtccagag cattaactga ttggaagtaa cgtgtaaata gagtggatac ctgcgccatt
     4261 ggcgccaatg ctcttccggt caatatggta caggcaatta atccaccggt agttaactca
     4321 ccttcggaga tcatataaac acctgcaata ataatagcga tagtcgcaat ttgttgggcc
     4381 agattagtga tactgatact tgtattggct acaaaacgta attgcataga gtttttagaa
     4441 gattcattaa ttattctttc ccaccgtgat tgcaggactg attctgctcc agttgtcttt
     4501 acagtttcta tagtagctaa agactcaaac aacgtcgctt gtttttca
//
LOCUS       DACTJE010000206         4518 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.208, whole genome shotgun sequence.
ACCESSION   DACTJE010000206 DACTJE010000000
VERSION     DACTJE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.208"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            22..249
                     /locus_tag="I9760_002770"
     CDS             22..249
                     /locus_tag="I9760_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5044516.1"
                     /translation="MPTHEIANLIHYYRKKSGLSQQELAHLAGVGKTVIYDIEKGKES
                     VRLNTLLKVLDVLNIQMKFETPFPQTTDNNS"
     gene            246..555
                     /locus_tag="I9760_002771"
                     /pseudo
     CDS             246..555
                     /locus_tag="I9760_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin HipA"
     gene            552..1470
                     /locus_tag="I9760_002772"
                     /pseudo
     CDS             552..1470
                     /locus_tag="I9760_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
     gene            1599..4166
                     /gene="mgtA"
                     /locus_tag="I9760_002773"
     CDS             1599..4166
                     /gene="mgtA"
                     /locus_tag="I9760_002773"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAT5044517.1"
                     /translation="MKINLDLNDLSTYDQNLLLNELNSSLEGLNNFQAQKRLTQYGTN
                     EILRKPYHLIILEAISHSINPLVAILFFAALISAFTGNVVNAAIIISIIIISIGLDYF
                     QSHRALVAIKMLQKKIATTVTVLRENQWLEIPAKELVPGDLFRLSAGDMVPADSILLK
                     SKDLHIHQAALTGESMPVEKEAIPLKTKPKNPLDALNIVFSGSSVIGGSATALAINTG
                     SNSLFGHIAESLKKKPPHTEFEQGMMRFSTFIMKMVFLLVIFVFSINIYMHRPLLDSL
                     LFAVALAVGLTPELLPMITTVTLASGAVRMSKKKVIIKNLSAVQNFGSIDILCSDKTG
                     TLTSGEMNLTKYLDFSGKQSEFVMLLAYLNSLYITEIKSPFNIAVLKKARLNPLDLAI
                     LKHDHPDIQPYHKVDEIPFDFERRRSSVIVSKNENHLFICKGAPENIMSVCSYYDFAG
                     ERELFTEKEQKQCELLFQSLSSEGYRVLAIAYKLMDRQLKYTQSDEKEMIFAGFLAFT
                     DPLLEDIPKVIKDLRQEGINIKILSGDNLIVTQHICQTVGMDTSRILTGEEISKISDD
                     ALPSLAEGIDIYARINPMQKQRIISALKKRGHVVGYLGDGINDVPSLHHADVGISVAS
                     AVDIAREAADIVLLEHHLKVLLNGIIEGRKSFRNVMKYLMMGTSSNFGNMISMAVAVL
                     FLPFLPMLPRQILLNNLLYDISQITIPTDHVDPSSIHQYKRLDINIIRQFMFYVGPIS
                     SVFDFLTFFVMLKIFSANEALFQTGWFVESLATQTLVIFIIRTAKKPWQSMPSLPLTA
                     TVLLIVMVGIWLPFSPFSGLFGFVPLPPLYFLFLVTATFTYLFLVELIKKKIMRKLY"
BASE COUNT         1538 a          778 c          777 g         1425 t
ORIGIN      
        1 ataattaatt tttggtgttc catgcccaca cacgagattg ccaatttaat ccattactac
       61 cgtaaaaaaa gcggcttatc acaacaagag ttagcccact tagcaggtgt tggcaagaca
      121 gttatatatg atattgaaaa agggaaagaa tcggtgcggt tgaacacctt gctaaaagta
      181 ttagatgttt tgaatatcca aatgaaattt gaaacgcctt ttcctcaaac aacggataat
      241 aattcatgag aaaagcatac gtatcagtga atggcatcaa ggctggaata ttagaagaat
      301 tacaaagtgg aaaatatcaa ttcagttact ttgataatta tcacggggca cctgtttctc
      361 tcacaatgcc gttaaccaat aaggtctatg attttgatgt atttccccct tttttttgaa
      421 gggttacttc ctgaaggcat tatgcttgaa gcactattac gcaaatataa aatcgataaa
      481 aatgattatt ttggccaatt gataatagtt ggccaagatg tcgtaggcgc agtgactatt
      541 gaggaaataa gatgaaacgc tgccccatta catatgaaaa aatcagtgac caagaaaact
      601 attcacaacg tggattgcgc ttgctttctc ctcaattaaa aaacctgagc ccattagatt
      661 taagtgctga tgagcagcgt caggaagcaa ttgctcgagt aggaaagatg tctattcaag
      721 gtgtccaaaa gaaactaagc acaaaactaa agattaaaga gggatgtttt gaaatcgtgg
      781 atcaaaatgg tgactatatt ttaaaaccac aaagtgatat ttatccagaa ttgccggaaa
      841 acgaagccat taccatgacc cttgcaaaga ccattggcct tgaatggttt agtgtactct
      901 aaagataaca gtttgactta cttcattaaa cggtttgata gaataggcca taataaaaaa
      961 ttagctttag aagattttgc acagctatcg ggtgaagatc ggcatacaaa atataaaagt
     1021 tctatggaaa acgtgcttgc aatcatagaa cagttttgta cattccccaa aattgaattc
     1081 gtgaaattat ttaagttgac attgtttaac ttcctggtcg gcaatgaaga catgcactta
     1141 aaaaattttt ccctaattac cagagataga aaaatttcca tatcgccagc ctatgattta
     1201 cttaacacta ttgctcaaaa aaatacaaaa gaagaaatcg ctttaccctt aaaggggaaa
     1261 aaaaataatt tgataacgaa tgattttctt aaatactttg ccgttgaaaa attaggatta
     1321 aatcagaagg tcatagatgg gatcgtacaa gagttccatc aaataataac tgaatggcga
     1381 aagctggttg gcttcagttt tttatctcaa cagatgcagg aaaaatatct ccaattgcta
     1441 gaacaacgat gcaagcgatt aaatttttga ttaactgata tgatgtctct tctttcccaa
     1501 acatcaattt aattttgttt cctataattg tatttagact ttcttgatca attctctgct
     1561 ttttattttt tacttttaag actaatggaa aaaagtaaat gaaaatcaac ttagacttaa
     1621 atgacttatc aacttatgac cagaacctac ttttaaacga gttaaatagt tcattggaag
     1681 gactaaacaa tttccaagcg caaaaacggt taactcaata tggtacgaat gaaattctaa
     1741 gaaaacccta tcatctgatt atccttgaag caatctcgca ctcaattaat cctttagttg
     1801 ccatcttatt ttttgctgct ttaatttcag catttacggg aaatgtagtt aatgcagcga
     1861 taataattag catcatcatc attagcattg gcctggatta ttttcaaagt catcgcgctc
     1921 ttgttgcaat taaaatgtta caaaagaaaa tagcaactac agtaacagtc ttacgtgaaa
     1981 atcaatggct ggagatacct gcaaaagaat tagttcctgg tgatttattt cgattatccg
     2041 ctggtgatat ggtgcctgca gactcaatac tattaaaatc aaaagacctt catattcacc
     2101 aagctgcact taccggggaa tcaatgccag ttgaaaagga agcaatccct ctaaagacca
     2161 aacctaaaaa tcctcttgat gcactcaaca tcgttttttc aggctcatct gttattgggg
     2221 gtagcgcaac agcactagca attaatacag gaagtaatag tttatttggt cacatcgcag
     2281 aaagcttaaa gaagaaacca ccacatacag aatttgaaca aggtatgatg cggttcagca
     2341 cttttatcat gaaaatggta tttctgttag taatctttgt attttcaatc aacatataca
     2401 tgcacagacc attgcttgat tccttgttgt ttgctgtagc gctagccgta ggtcttactc
     2461 ctgaattact tcccatgatc acgacagtga ccttagcctc tggtgccgtt cgcatgtcaa
     2521 agaaaaaggt aattatcaaa aatttatcag cggtccaaaa cttcgggagc attgatattt
     2581 tatgtagtga taaaacaggc accctgacaa gcggagaaat gaatttaaca aaatacctgg
     2641 atttttcagg aaagcaatcg gaatttgtaa tgttattagc ctacttaaac agcttataca
     2701 taacagaaat taagagtccc tttaatattg cagtattaaa aaaagcaaga ttaaatccat
     2761 tagatttagc aatcttaaaa catgatcatc ccgatattca accataccat aaagtagatg
     2821 aaattccttt tgattttgag cgcagacgtt caagtgtcat tgttagcaaa aatgagaatc
     2881 acctgttcat ttgcaaaggc gcacctgaaa acattatgag cgtatgctcc tattatgatt
     2941 ttgcaggcga aagagaactg tttaccgaaa aagaacaaaa acaatgtgaa ttgcttttcc
     3001 aatcgttaag cagcgaaggt tatcgagtct tagctattgc ctacaaacta atggatcgac
     3061 aacttaaata tactcaaagt gatgaaaaag aaatgatctt tgcgggtttt ttagccttta
     3121 ctgatcccct acttgaagat atcccaaaag taattaaaga cctgcgtcag gaaggaatca
     3181 atataaaaat cttatctggt gataatttaa tagttactca acacatctgt caaacagttg
     3241 gtatggatac aagccgtatt ttgacagggg aagaaatttc aaaaatttcg gatgacgcac
     3301 ttccctcact agcagaagga attgatattt atgctcgtat aaaccctatg caaaaacaga
     3361 ggataatctc tgctttaaaa aaaagagggc atgtcgtggg atatcttgga gatgggataa
     3421 atgatgtacc atctttacat cacgctgatg tgggaatttc agttgcttct gctgttgata
     3481 ttgctcgtga agcagcagat attgttttgc tggaacatca tctaaaagta ttattaaacg
     3541 gaatcatcga aggtcgtaag tcgtttagaa acgtcatgaa atatctcatg atgggtacaa
     3601 gctcaaattt tggcaatatg attagtatgg cagttgcagt cctgttttta ccttttttac
     3661 ctatgctacc cagacaaatc ctattaaaca atttacttta tgacatctca caaataacga
     3721 tacccactga tcatgtagat ccaagctcga tacatcaata taagagatta gatattaata
     3781 taattcgcca atttatgttc tacgttgggc ctattagctc tgtatttgat tttttaacat
     3841 tttttgtcat gttaaaaata ttttctgcta atgaagcttt gttccaaaca ggttggtttg
     3901 tcgaatcttt ggcaacacaa actttagtta tttttattat tcgcacggcc aaaaaacctt
     3961 ggcaaagtat gccaagtctc cctttaacag ctactgttct actgatagtt atggtgggaa
     4021 tatggttacc atttagtcca ttttctggat tatttggatt tgtgcccttg ccgccattgt
     4081 attttctttt tttggttaca gcgactttta cttacttatt tctggttgaa ttaattaaaa
     4141 aaaagataat gcggaaatta tattagtcaa acaacattat cattcatttg aaggctcaaa
     4201 atgattaaat ggatttctgg gtttataatt ttggttgtac ttattattat cattggtcat
     4261 tttagtcaac tgcgagtttt tcttggttta attgagcaaa aggataaaaa atatatttca
     4321 tggccatttg ttttactatt tctaatttag aaaccattat tttccaaatg aatacattct
     4381 ctaaaaaaat caaaccgtgc aaaatacatt cacttagact taatttagga atataatatg
     4441 gaaaataaaa caatggcatt aaattttctt aaccgcattg aacgatgtct tgtaccccaa
     4501 tcaaatcctc aaataatg
//
LOCUS       DACTJE010000207         4505 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.209, whole genome shotgun sequence.
ACCESSION   DACTJE010000207 DACTJE010000000
VERSION     DACTJE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.209"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1198)
                     /gene="lem9"
                     /locus_tag="I9760_002774"
     CDS             complement(<1..1198)
                     /gene="lem9"
                     /locus_tag="I9760_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem9"
                     /protein_id="HAT5044518.1"
                     /translation="MIAILNLNCQKQNTLLLRYKIMKHLAELKKLKEKCLEMSDKEFN
                     YCFNKGDTSQRSIGTIIARLMNRIDLKDEFFKTDKDYVDWIEDVISARKKAALSPSST
                     PVSMDKKTPDSATELAKIKEKCLNMNEKEFNYCFNKGDASERGIALIIARLKNAVGLK
                     DEFFKTDKDYVDWIEDIISARKKAALSPSSTPVSMDKKTPDSATELAKIKEQLLKMKD
                     GEFDYYVKDLSTDISNGGISISRRLLRDLGLKGEFFKTQKDYNEWIDDVISTRKFINF
                     SANIDNILNKFEEKIKNIGTNYPEAKIKANDLLMSLRKNKDEAFSNPSLESLYDFADK
                     SKQMIKSTISSLERESGSVVFLSDLAEQILNTINTFLNNTLSSSTSSRSGFFGFKLSY
                     EKVIVQD"
     gene            complement(1309..2634)
                     /locus_tag="I9760_002775"
     CDS             complement(1309..2634)
                     /locus_tag="I9760_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAT5044519.1"
                     /translation="MSVLYYNLNNHYASHFVYSPEKRANHDNNPEKIMKKPLDTTEQN
                     RIAALYELKILDTPPDERFDRIIRLTSGYLSVPIAFISFIDSEHQWFKSSCGIGLNKI
                     SRNDSFCHYTIQQNKPMIISDTLLDERFCNNPYVTSEPNIRFYAGFPLSTVDGFNVGT
                     LCAIDKKPRNLDETEIQILKDLATLAEDQLNLLDISLLEKSVREKNISLIKAKKELEM
                     RNNFIRKVFGSFMSDEIVAALLESKSELKLGGEERKITILFSDLRNFTTLSEHFPAEK
                     IVAALNNYYGKMVDVIEKYNGTVDSFIGDAIMVVFGAPHSGGDDAYRAIACALEMQVI
                     MQEVNAMNRNSSLPELAMGIGINTGYAIVGNIGSQKRMQYSAIGSPVNLASHIQDLTL
                     GGQILISEATYQEVNHSLELNGHLRVKVKGVAAPITIYDVIGLNKKSDS"
     gene            complement(2799..3830)
                     /gene="ravX"
                     /locus_tag="I9760_002776"
     CDS             complement(2799..3830)
                     /gene="ravX"
                     /locus_tag="I9760_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavX"
                     /protein_id="HAT5044520.1"
                     /translation="MRRSKAIQMPKKRTQILTISESSKEDLDVVPEYSEEMDVSPSKE
                     TSKTNSKKLESTARKEQIKKLEGKLYSHIQDAIELEEESFAEFSINTGRPSNYLFRFV
                     SEKEYLAILENLNQGKRIFGNKKKVFEEEDEKISTKAKGETFFAFDLKYAQKYFNKGT
                     RRNGVMLAIELHEDADSKLLSNTEIVRRIGDDPGMAGGRWAKCKKVKPGERGVVVAKC
                     EGVRAGCKYPYTLGFREGEEGVSPFDTLFELIRSVKVLGNNGSCKTIYHCSVPAPKEK
                     ELTSIVKDAESGIDEIVSDEILSVDSSSPTSRFGFFTNKELSGIPRLVNKAPEHEILP
                     SDDKTSLHH"
     gene            complement(4041..>4505)
                     /locus_tag="I9760_002777"
     CDS             complement(4041..>4505)
                     /locus_tag="I9760_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044521.1"
                     /translation="QRIKLEEEHKSKKYFAQSDAIDLILNNFENEINSIGAYYAPAKQ
                     RAIELLDTLRKYKEEAFNDPSREKLISFAQSTKRAIQEATPILQKDLGWGDYLTNLAK
                     QLVNAVTFAVAYAVTFGTTGHQGFFALKSSLAVSQSQNLEGAINSELSQKNC"
BASE COUNT         1332 a          876 c          698 g         1599 t
ORIGIN      
        1 ggtcttggac aattactttt tcataggaca acttaaaacc aaaaaaacca ctacgactgg
       61 aggtacttga gcttaatgta ttatttagaa atgtattgat agtatttaga atttgctcag
      121 cgagatcact caaaaaaacc accgatcctg actccctctc taaagatgaa atggtgcttt
      181 ttatcatttg tttcgattta tcggcaaagt catagagtga ctctagagaa ggatttgaaa
      241 atgcctcatc tttatttttt ctaagcgaca tcagcaagtc attggctttg atttttgcct
      301 caggataatt agtccctata ttttttattt tttcttcaaa tttatttaga atattatcaa
      361 tattggcact aaagttaata aattttcttg tggaaattac gtcgtctatc cactcattat
      421 aatctttttg tgtcttaaaa aactccccct taagacctaa atctcgtaaa agtcgtcggc
      481 taatagatat gcctccgttg gatatatcag tacttaaatc tttaacataa taatcaaatt
      541 caccatcttt cattttcaat aattgttctt ttatctttgc taattccgtg gctgaatcag
      601 gtgttttttt atccatagat actggagtgc tgctgggaga tagagccgct ttttttcttg
      661 cagaaattat gtcttctatc cagtcaacat aatctttatc tgtcttgaaa aactcatcct
      721 tgaggcctac tgcattcttg agccgcgcaa tgattaaagc aatccctcgt tctgaggcat
      781 ctcctttgtt aaaacaataa ttaaattctt tctcattcat gtttagacat ttttctttaa
      841 tctttgctaa ttccgtggct gaatcaggtg tttttttatc catagatact ggagtgctgc
      901 tgggagatag agccgctttt tttcttgcag aaattacgtc ttctatccag tcaacataat
      961 ctttatctgt cttgaaaaac tcatccttaa gatctattct gttcataagc cgcgcaataa
     1021 ttgtaccaat gctgcgttgt gaggtatctc ctttgttaaa acaataatta aactccttat
     1081 cactcatctc cagacatttt tctttaagtt tttttaattc agctaaatgt ttcataattt
     1141 tatatcttag taaaagtgta ttttgctttt gacaatttaa atttaatatg gctatcacat
     1201 gacattatag cattttatat gacaaaaatt gcaaatattg gactatttgt atagaatttt
     1261 tggaactttc catttgcccc atgattttaa aaagtgatat tattacttct atgaatctga
     1321 ttttttattc aaaccgatta catcataaat agtgatagga gcagcaactc ccttaacttt
     1381 tactctgaga tgcccattta actcaagcga atgattaact tcctggtaag ttgcctcaga
     1441 aattaaaatt tgcccaccca aagtcagatc ctgaatgtgt gatgccagat taaccggaga
     1501 accaattgca ctgtattgca tacgcttctg agaaccaatg ttccctacaa ttgcgtaacc
     1561 tgtattaatc cctatgccca ttgctaattc aggcaagctg ctgttacggt tcattgcatt
     1621 cacctcttgc atgatcacct gcatctctaa tgcacaggct atcgctcggt atgcgtcatc
     1681 acctccagaa tgtggtgcgc caaatactac cataatggcg tcaccaataa aggaatcgac
     1741 tgtgccattg tatttttcta tgacatcaac catttttccg taataattat taagggcagc
     1801 gacaattttc tcagcaggaa agtgctccga caaagtagta aaattacgta aatcactaaa
     1861 cagaattgta attttccgct cctcccctcc taattttaac tctgattttg attcaagaag
     1921 cgctgcaaca atctcgtcag acataaaact accaaatact ttacgaataa aattatttcg
     1981 catttcaagt tcttttttag cttttatcag agagatattt ttttctcgaa cagacttttc
     2041 gagaagcgaa atatccaata ggttgagttg atcttctgcc aaagttgcca aatcttttaa
     2101 aatttgaatt tcagtctcat ctaaattgcg tggtttttta tctattgcac ataatgttcc
     2161 tacattaaaa ccatcaactg ttgacaacgg aaatcctgca taaaaacgaa tattgggttc
     2221 actagtcaca taggggttat tgcaaaatcg ctcatccaac aaggtgtctg agattatcat
     2281 tggtttattt tgttgaatag tatagtggca aaatgaatca ttacgggata ttttgttaag
     2341 acctattcca caactggatt tgaaccattg gtgttcagaa tcaataaaag aaataaaggc
     2401 aataggaacc gataagtagc ccgaagtgag tcggataatc cgatcaaatc tttcatctgg
     2461 tggtgtatcc agaattttta attcgtataa tgcagcaatg cgattctgct cagtagtatc
     2521 caaaggtttc ttcataattt tctcaggatt gttatcatga ttggctcttt tctcaggtga
     2581 ataaacaaaa tgagaggcat agtggttatt aagattatag tacaatacac tcatattaat
     2641 tggcttaaac caacgaataa gctaattgta tttagcaatg gaaataaagt tatgggttac
     2701 ttgtatgtgg ctaaccctta ttatggagga tatatgcaga aacgggcaaa taaatagtat
     2761 ttaattaccc gtttaaagtc ttaaaaattt aacctgattt aatgatgcaa ggaggtctta
     2821 tcatcagagg gtaatatttc atgttcaggt gctttattta ccaatcgtgg aatacccgag
     2881 agctccttgt ttgtaaagaa accaaagcgt gatgtaggag aacttgaatc aactgataaa
     2941 atctcatcac taacaatctc gtctatacca ctttcagcat ctttcacaat acttgtaagc
     3001 tctttttctt ttggcgcagg tacagagcaa tgataaatag ttttacaact gccattgtta
     3061 cccaggacct ttacagaacg tattagctca aatagtgtat cgaaaggact aaccccctct
     3121 tctccttctc taaaacccaa ggtgtatgga tacttacatc ctgcgcgaac tccttcacat
     3181 ttagctacta ctacccctcg ttcaccaggc tttaccttct tgcatttagc ccatcggcca
     3241 ccagccattc cgggatcatc tccaattctt ctaacaattt cagtattgga taatagttta
     3301 ctatcggcat cttcgtgcaa ttctatagca agcatcactc cattacgtcg tgtgccttta
     3361 ttgaaatatt tttgagcata tttaagatca aaagcaaaaa aagtttcccc ttttgcttta
     3421 gttgatatct tttcgtcctc ttcttcaaat acttttttct tgttaccaaa tattctcttt
     3481 ccttgattta aattttcaag aattgccaaa tattcttttt cactgacaaa gcgaaataaa
     3541 taattactag gtcttccagt gttaatgcta aattctgcaa aagattcttc ttctaattcg
     3601 atagcatctt gaatatgact gtaaagcttt ccttccaatt tcttgatctg ttctttacgg
     3661 gcagtactct ccagtttttt actgttagtt ttgcttgttt ctttgctggg agatacatcc
     3721 atctcctcag aatactcggg tacaacatcc aaatcttctt tacttgattc tgaaatagtt
     3781 aatatttgtg ttcttttttt aggcatttgt attgctttac ttctacgcat ctaactctcc
     3841 taacatcgtt acgatataca atgtgtgctt atattattct taccaaatct attttgcaca
     3901 ttatcagcaa aatgaaatgg attgcaatct ttcatttcta aatcaaaatt accgaatcga
     3961 aatacattag atttaagaag aatgcttggg cagcaaaaat aatgtcttgc tcattagcat
     4021 tctatgtcta gcctaattcc ctaacagttt ttttgactca attcgctatt gattgcccct
     4081 tcaagatttt gagattgact aaccgccaga gaagatttca aagcaaaaaa gccttgatgc
     4141 ccggtggttc caaaagttac agcataagcc acagcaaagg tcactgcatt aaccaattgt
     4201 ttcgctaaat tggttaaata atctccccag cctaaatctt tttgcagtat tggtgttgct
     4261 tcctgaattg ctctttttgt actttgcgca aaagagatta atttttctct gcttggatca
     4321 ttaaatgctt cctctttata ttttctcaaa gtatctagca attctatagc tctttgtttt
     4381 gcaggggcat aataagcacc aatagagtta atttcatttt cgaaattatt caaaatgaga
     4441 tcaatagcat cggactgagc aaagtatttt ttgcttttgt gctcttcctc aagtttgatt
     4501 cgttg
//
LOCUS       DACTJE010000208         4333 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.210, whole genome shotgun sequence.
ACCESSION   DACTJE010000208 DACTJE010000000
VERSION     DACTJE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.210"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            39..809
                     /locus_tag="I9760_002778"
     CDS             39..809
                     /locus_tag="I9760_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HAT5044522.1"
                     /translation="MKKIITILQARYSSTRLPGKVLFPILGQPMLIQQINRIKNSTLH
                     GQIVIATSTHHEDEKIVNCCKENGITYYCGKLDDVLDRFYQAASLYQPDHIVRLTGDC
                     PLIDPQIIDEVIQTHLDGNFDYTTNALKPTYPDGLDVEVFKFSALKTAWIEATLNSDR
                     EHVTPYIYRNNDQFNIGHLYNDIDLSHLRWTVDWQIDFDLIKRIFENLFPYNPRFTFS
                     DILQFIESNPELKTINTCHTRNEGLQKSLQEDQLISRA"
     gene            814..2103
                     /locus_tag="I9760_002779"
     CDS             814..2103
                     /locus_tag="I9760_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAT5044523.1"
                     /translation="MYKCYEASERFLERALESIPLGSQTFSKSYVQFPKGVSPLFLQR
                     GAGCKVWDLDGNEYTDFVNGLLAVTLGYNDPDVNASVLEQLQNGVTFSLAHPLETMVA
                     EKLIETIPCAEMVRFGKNGSDVTSGAIRLARAYTNKDRIAVCGYHGWQDWYIGTTPRN
                     LGVPKSTQELTHTFIYNDIDSLDHLFSAYPGQFAAVIMEPMNIEQPKSGFLESVKELA
                     HHHGALLIFDEMITGFRFAIGGAQNYFNIIPDLATFGKGMANGYPLAALVGRKDIMQL
                     MTEIFFSFTFGGETLSLAASYATINKIQSQPVIEKLFTLGTQLKEGIHQLIDKYSMVD
                     FLSCVGHPSWSFLIFKDIEPYTSWDIKTLWMQEILAKGILSFGTHNLSYAHNECDIDN
                     LLKIYNEVFPLLREAVVQKNLHKLLRAEPLQPLFKVR"
     gene            2114..3193
                     /gene="pseG"
                     /locus_tag="I9760_002780"
     CDS             2114..3193
                     /gene="pseG"
                     /locus_tag="I9760_002780"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HAT5044524.1"
                     /translation="MNIVFRLDSSNLIGSGHVMRCLALANALRERKVKVLFICCNLIG
                     NSIRVIKQNGFEVIELFNKPNIQWEQDAKETISYLKQMEKPDWIFLDHYNLDIKWEKE
                     IRPFVNQIAVIDDLANRQHDCDLLIDQNYYDKKICRYSNLVPKSCTKFIGPRYALLRS
                     EFKEARSLLKARSGEVQQILVFLGGNDAGNVTSFVLSAIEEIPLLNNKEMHVVVGGSN
                     PNRDKIREKCEKKGYHYYCQINNIATLMAKSDLAIGAGGLSTWERCSTGLPSLVVSLA
                     HNQDVLVDNALSTNIFEYAGQANNFHYTSFKKQLLELILDSEKLRKMSINGLNLVDGL
                     GSDRIANYLLYGTTNNGNNSTLCNN"
     gene            3147..3611
                     /locus_tag="I9760_002781"
     CDS             3147..3611
                     /locus_tag="I9760_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5044525.1"
                     /translation="MEQLIMEITLRSATINDASNLFEWRNALETRRYSSNPNPIEMAD
                     HLRWLQHSLVLPNRRLLIAEYQNNPIGVLRFDIDALLEQAEISIYLVPGLAGKGWGTK
                     ILESGTEWIKNNIPQVKNLVAKILADNVRSQRAFEKSGFNPTTYIYEKSIQN"
     gene            3698..>4333
                     /locus_tag="I9760_002782"
     CDS             3698..>4333
                     /locus_tag="I9760_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044526.1"
                     /translation="MNKLKTYRDYLIKKLILVLFLFVLVFIPIELFSRWVSVNSIAPF
                     GSWFSRDQKFLIADTINGGIVAIGDSIIARNFYPELFYSSLTEKLSIIYPTYNLGLEG
                     ASQEEQLALYKYLLKRKVNPKLVIIDVPVHILNVDNFYIASSGDKTPLKLWDDNVSDY
                     YTRCEVSDTYAISTKIYCKLASKLYFLRLLNFYHQQINNSDKTLLHTSKSRL"
BASE COUNT         1449 a          757 c          774 g         1353 t
ORIGIN      
        1 attatggcaa attatcctta ataagaattg atactctaat gaaaaaaatt ataacaattc
       61 tacaagcaag atatagctct accagattac ctggaaaagt tctatttccc attcttggcc
      121 aacctatgct aatacagcaa attaatcgca ttaaaaattc aacactacat ggccaaatag
      181 tgattgcaac aagcactcat catgaagatg aaaagattgt caattgctgt aaagagaatg
      241 gtatcactta ctattgtggc aaacttgatg acgttttgga tcgtttctac caggcagcta
      301 gcctatacca acctgatcat atcgttcgtt taactggaga ttgtccatta atagacccac
      361 agattattga tgaagtgata caaacccatc ttgatggcaa ttttgattat acgactaacg
      421 ccttaaaacc aacttatcct gatggcttgg atgtagaagt atttaaattc tccgccttaa
      481 aaacagcctg gattgaagca accttaaatt cagatagaga gcacgtcact ccctatattt
      541 atcgcaataa tgatcagttc aatattgggc atctctataa tgatattgat ttgtctcatt
      601 tgaggtggac tgttgactgg caaattgatt ttgatctgat aaaaaggata tttgagaatt
      661 tattccccta caatccaaga tttacttttt cagatatctt acaattcata gaaagtaatc
      721 cagagttaaa aacaatcaat acttgccata caagaaatga gggcctgcaa aaatcattac
      781 aggaagatca actaatttca agagcataaa gcaatgtaca aatgttatga ggcatccgaa
      841 cgatttttag aacgtgcatt agagtcaatc cctttaggtt cacagacatt tagcaaaagt
      901 tatgttcaat ttcccaaagg tgtatcccct ttatttttgc aacgaggtgc aggttgcaag
      961 gtatgggatt tagacggaaa tgaatacact gactttgtaa acggattatt ggctgtcact
     1021 cttggataca atgatccaga tgtcaatgca tctgttcttg agcaacttca aaatggggta
     1081 accttttctt tagctcatcc tcttgaaaca atggttgcag aaaaactgat agaaactatt
     1141 ccttgtgctg aaatggtaag gttcggaaaa aatggttcag acgttacctc tggtgccata
     1201 cgcctggctc gcgcatatac taataaagat cgtattgccg tttgtggata tcatggttgg
     1261 caagactggt atattggtac aacccctcgt aatttgggag ttcccaaaag cacacaagaa
     1321 ttgactcata catttattta taatgacatt gatagtcttg atcatttatt ttcagcttat
     1381 ccaggccagt ttgctgctgt aattatggag ccaatgaata tagagcaacc caagtctggt
     1441 tttttggaat cagttaaaga acttgctcat catcatgggg ctctgcttat ttttgatgag
     1501 atgataactg gctttcgatt tgcgattgga ggagcacaaa actactttaa tataatccct
     1561 gatttagcca cttttggtaa aggaatggct aatggatatc cactcgcagc attggttggt
     1621 cgtaaagaca ttatgcaact catgacagaa atatttttct cattcacatt tggtggtgaa
     1681 acattatcac ttgccgcctc ttatgcgact atcaataaaa tccaatctca gccagtaata
     1741 gaaaagctat ttactctagg cactcaactg aaagaaggta tacatcaact tattgataaa
     1801 tactctatgg tggattttct tagctgtgta gggcatcctt cgtggtcatt tctaatattt
     1861 aaagatattg agccatatac ctcatgggat attaaaacat tatggatgca agagattctt
     1921 gcaaaaggga tcttaagttt tggaactcat aatttatcat acgcgcataa tgagtgtgac
     1981 atagataatt tgttaaaaat atataatgaa gtattcccac ttcttagaga agcagtcgtt
     2041 cagaaaaatt tacataaact attgcgtgct gaaccattac agcccttatt taaagtacgt
     2101 tagttaatac acgatgaata tagtatttcg gcttgattcc tcaaacttaa ttggctctgg
     2161 ccatgtgatg aggtgcttag cattagcaaa cgcgcttcgg gaaaggaaag taaaagtcct
     2221 atttatttgc tgcaatctga ttggaaatag tattcgggtc ataaagcaga atggttttga
     2281 agtgattgaa ctctttaata aaccaaatat ccaatgggag caggatgcta aagagaccat
     2341 atcttatctg aaacagatgg aaaaacctga ctggattttt ttggaccatt ataatctgga
     2401 tataaaatgg gaaaaagaaa ttcgtccgtt tgtaaatcaa attgcagtca ttgatgattt
     2461 agctaacaga cagcatgatt gtgatctctt gatagatcaa aattattatg acaaaaaaat
     2521 ctgtcgttac agtaatctag tccccaaatc ctgtacaaaa tttatagggc cacgttatgc
     2581 cctgctgcga tcagaattca aagaagcacg ttcattatta aaagcccgct ctggagaggt
     2641 acaacaaatt ttagtatttt tgggaggaaa tgacgcaggc aatgtaactt ctttcgtatt
     2701 atccgctatt gaagaaatac ctcttcttaa taacaaagag atgcatgtgg tagttggagg
     2761 atctaaccca aatcgagaca aaatccggga gaaatgtgaa aaaaaaggtt atcactatta
     2821 ttgccaaata aacaacatag ccactttaat ggctaaatcc gatttagcta ttggtgcagg
     2881 gggattgtct acctgggaac gttgttccac aggcttacca agtttagtcg taagcctcgc
     2941 acacaaccag gatgttctag ttgataatgc cctctcaaca aatattttcg aatatgctgg
     3001 acaagccaat aactttcatt atactagttt caaaaaacaa ttattggaac ttattttaga
     3061 ctctgaaaaa ctcaggaaaa tgagtattaa tgggctcaat ttagtagatg gattgggctc
     3121 tgatcgaata gctaactatc tgctgtatgg aacaactaat aatggaaata actctacgct
     3181 ctgcaacaat taatgatgca agcaacctgt ttgagtggcg caatgcttta gagacaaggc
     3241 gttattcatc caatccaaat ccaatagaaa tggcagatca ccttcgctgg cttcaacata
     3301 gtttagttct tccaaatcga cgattgttga ttgctgaata tcaaaataac cctataggcg
     3361 ttttacgatt tgatattgat gctctccttg aacaagccga aatttcgatc tatttagttc
     3421 ctggattggc aggcaaaggg tggggaacga aaatacttga atcaggtaca gaatggatta
     3481 aaaataatat accgcaggta aaaaatttag tcgcgaaaat actggctgac aatgttcgct
     3541 ctcaacgtgc ttttgaaaaa tctggattta acccaacaac ttatatttat gaaaaatcga
     3601 ttcaaaacta aattagtagc aagaaaaaat ttaattatta agatacaatt attaaatttt
     3661 atcagcttaa taatttctca tagcttgaag tactaatatg aataaattaa aaacatatcg
     3721 cgactacctg ataaaaaaac taattttggt tttattttta tttgtccttg tatttatccc
     3781 aattgagtta ttttccagat gggtatcagt aaactctatt gccccattcg gaagttggtt
     3841 tagccgtgat caaaaattcc tcatcgctga tactattaat ggtgggatcg ttgctatagg
     3901 tgattctatt atcgcaagaa atttttatcc ggagcttttt tattcatccc taacagaaaa
     3961 actatccata atatacccaa cttataattt agggcttgaa ggagcaagtc aggaagagca
     4021 attagcgctc tataaatatc ttctaaaaag aaaggtaaac ccaaaattag ttattatcga
     4081 tgtacctgtt catattctta atgttgacaa tttttatata gcctcttctg gggacaaaac
     4141 acctttaaaa ttatgggatg acaatgtttc tgattactat actcgctgtg aagtaagtga
     4201 tacctatgcc atttccacaa aaatatattg taaattggca agtaaactct attttttaag
     4261 attactgaat ttttaccatc agcaaattaa caatagtgat aaaaccttgt tacatacatc
     4321 aaaatcacga tta
//
LOCUS       DACTJE010000209         4315 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.211, whole genome shotgun sequence.
ACCESSION   DACTJE010000209 DACTJE010000000
VERSION     DACTJE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.211"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(161..1285)
                     /locus_tag="I9760_002783"
     CDS             complement(161..1285)
                     /locus_tag="I9760_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RalF"
                     /protein_id="HAT5044527.1"
                     /translation="MHPEIEKAQREIIEAFNAKPKNGINKIKEICEQYKISPNEEIAE
                     FFHQQRKNLDLEAVGDYLSSPEAENQQVLKAFTSQMNFNGQSFVEGLRTFLKTFKLPG
                     EAQKIDRLVQSFSGAYFQQNPDVVSNADAAYLLAFQTIMLNTDLHNPSIPEKNKMTVD
                     GLKRNLRGGNNGGDFDAKFLEELYSEIKAKPFELNFVKTSPGYELTSTTLNKDSTFKK
                     LDSFLHSTDVNINTVFPGIGDNVKTTVDQPKSWLSFFTGYKGTITLTDNKTSAQATIQ
                     VYTPNIFSKWLFGEQPRVIIQPGQTKESIDLAAKAAADFSSPVKNFKATYDYEVGDLI
                     KAYDNQKKLITIERNLALKEGVPKDPDAEMQKEKGRQLKF"
     gene            1578..2918
                     /gene="lem17"
                     /locus_tag="I9760_002784"
     CDS             1578..2918
                     /gene="lem17"
                     /locus_tag="I9760_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem17"
                     /protein_id="HAT5044528.1"
                     /translation="MAMEDESKVKKGLKEEKEQLNQKWLEQLLNNPRWGPSVVKLAMA
                     RKKENRNKRGEAFNCFVLSRPQNAFEEDEYRKFLDKLKNNINDFSGTTRFQIALFTAD
                     NHHWTAFDFFIVDGKLHVFCLDAANDSAGDAALDEIMSRFPDCDAYRLEPDRDPKAKD
                     SKHFRLIQTDRENCSRMTIEHILLLSKKKFLFNENFANYKVVNGVKLINPDALFRQSP
                     DLYRMTQIDSILDSLMIASPKEAQQLVSKKGLNPEQYRDLLGHTSGKQSKSSKNTTID
                     YKRQRVEKNLLAFLAESEQKHLEDIIDNSEKQWSEFLEMSKSERTQFLLGNIREKPFK
                     EIVGNILGKGIKDRLDHKKYIENDVIKKSSFTLFRGIDKHFEIKKIKAEINELEKALK
                     IVNQLSNDTSADEVLLHLQKQKLPLAVKGEMESAREIYTTVDREKSNLQSHKIN"
     gene            3227..4234
                     /gene="legLC4"
                     /locus_tag="I9760_002785"
     CDS             3227..4234
                     /gene="legLC4"
                     /locus_tag="I9760_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegLC4"
                     /protein_id="HAT5044529.1"
                     /translation="MGNLAMKLELNFLQKQTKEIIAELQKLKDSGVTHLDLSGNEFDK
                     RTGIELAAILSALKNSGVTHLDLSLNNFDKMPATDLVVMLSALKGSGITHLDLSGNDF
                     GEKSGAELVEILNAIKGSGITSLSLAENDLGTKTESELFDILSTLKDSGVTHLDLYGN
                     KLGERKGPELSTMLGALKDSGITHLDLSWNNFGKMAKTDLFVMLKAVNHTGITHLNLS
                     DNFLGTKSGKELSELFRQIEGKLTITIAKNKLEKCGCSAKEFARLLFDTSHHYVLGSS
                     EYDKAVSEEFHRLKKQHLVALLFTGKKLAEKKESFFYYEGGRRDVMSLISAKMPSIQI
                     I"
BASE COUNT         1427 a          697 c          827 g         1364 t
ORIGIN      
        1 aaagttaatt aaggataata atgtgaattt tgataaatta aataaatcaa tttgccctat
       61 aaaaaacaat ctcattcttc aaactatcga ggccatttca cccagatttg tggagtccaa
      121 acactatgag accgaatgat ttattcttag tctaattgga ttaaaatttt aattgtctac
      181 ctttttcttt ttgcatttca gcgtctggat ctttgggaac gccctcctta agtgccaggt
      241 ttctctcaat cgtaatgagt tttttctggt tatcatatgc ttttattaaa tcacctacct
      301 cataatcata cgtcgcttta aaatttttta ctggcgatga aaagtcagct gctgcttttg
      361 ctgccaaatc gattgactct ttggtttgtc caggttgtat tattactctc ggttgttctc
      421 caaatagcca ttttgaaaat atattgggag tataaacctg gatagtagct tgtgcggatg
      481 ttttgttatc tgtcaacgtt attgttccct tatatccggt aaaaaaactt aaccatgatt
      541 ttggttgatc tacagttgtt ttaacattat cacctattcc tggaaaaact gtattgatgt
      601 ttacatccgt tgaatgcaag aatgaatcca gctttttaaa ggtagaatct ttattaagag
      661 tggttgaagt aagttcatat cctgggctgg ttttaacgaa attcaactca aaaggtttgg
      721 ctttaatctc actgtatagt tcctctaaaa atttggcatc aaaatctcca ccattattac
      781 ctcctctgag atttcttttg agtccatcca cggtcatttt atttttctca gggatacttg
      841 ggttatgtaa atcagtatta agcataatgg tttgaaatgc taaaagatag gcagcatccg
      901 cattggaaac cacatcagga ttttgctgga aataggcacc actaaagctt tgcactaacc
      961 ggtcaatttt ttgagcttcg cctggtaatt taaatgtttt caaaaaggtc cttaaaccct
     1021 caacaaaact ttgcccattg aaattcattt gactggtaaa agcttttaat acttgctggt
     1081 tttctgcctc tgggctgctt aaataatcgc caaccgcttc taaatccaaa ttttttcttt
     1141 gttgatgaaa gaactcagct atctcttcat tgggagaaat tttatattgt tcacatattt
     1201 cttttatttt atttatccca ttttttggct tggcattgaa tgcctcaata atctctcttt
     1261 gggctttttc aatttctgga tgcataatct gctcctttag tctgaggaac cgctggattc
     1321 cttttaatag cggccaacaa tgatcttatt atagtctaat tgagtaaaaa caacttaatg
     1381 atcaaaaaat actacctgct tgcaagaaag gataatccgc aattttgagg tgtattttcc
     1441 ttctatttca ttctgcatgt attaagatgc caaataccag ttttaacagc cccactgaaa
     1501 atcccactca ttagaaaaac gtcgcagtgt gctgtataag ttctatacta gttaatatag
     1561 aatcagattg aagataaatg gctatggaag acgaatccaa agtcaaaaaa gggttaaaag
     1621 aagaaaaaga acaacttaat caaaagtggc ttgagcaatt attaaataac ccacgctggg
     1681 gtccttccgt ggttaagtta gctatggcaa gaaaaaaaga aaatcggaac aaacgtggtg
     1741 aggcatttaa ctgttttgtg ttgagcagac cacaaaatgc gtttgaggaa gatgaatacc
     1801 gaaaattcct ggataagtta aaaaataaca ttaacgattt tagtggaacg acccgatttc
     1861 aaatcgctct ttttactgca gataaccacc actggacagc atttgatttt tttatcgttg
     1921 atggaaagct tcatgttttt tgtttagatg cggctaatga tagtgcagga gatgctgcgc
     1981 ttgatgaaat tatgagccga tttcctgatt gcgatgctta ccggttagag ccggatagag
     2041 atccaaaggc gaaggattca aaacattttc gtttgattca aacagaccga gaaaattgtt
     2101 cacgaatgac tattgagcat attttgctgt tgtcaaaaaa gaaattttta tttaatgaaa
     2161 atttcgccaa ctacaaagtg gttaatgggg tgaagctgat taatcctgac gcactattca
     2221 ggcaatctcc agatctgtat cgtatgactc aaatcgattc tattctggat agtcttatga
     2281 ttgcttcacc aaaagaggcg cagcaactag tcagtaagaa aggtttgaat ccagagcagt
     2341 atagggattt gcttggacat acatcaggta aacaatccaa atcatcaaaa aatacaacca
     2401 tcgattacaa gagacagagg gtggagaaaa accttttagc atttttggcc gagagtgagc
     2461 agaagcatct ggaagatatt attgataact ctgaaaaaca atggtctgag tttttagaaa
     2521 tgtctaagtc agaacgcacc caatttttat tgggtaacat cagagagaag ccttttaaag
     2581 aaattgtcgg gaatattcta ggcaaaggca taaaagacag gctcgatcat aaaaaataca
     2641 ttgaaaacga tgtgatcaaa aaaagttctt ttactctttt tagaggaatt gataagcatt
     2701 ttgaaatcaa gaaaattaaa gcagaaatta atgaactgga aaaggccttg aaaatagtaa
     2761 atcaattatc taatgacaca agtgcggatg aagtattgtt acatttacaa aaacaaaaat
     2821 tacctcttgc ggtaaaagga gaaatggaga gtgcaagaga aatttatacg acagtagaca
     2881 gggaaaaatc caatctccag tctcataaaa taaattagtt tcagattatg tgttttgatt
     2941 cttaaaaacc gattaaggta ttgatatggt gtagaaatat attcagtgag atgttcttgg
     3001 ttagacaaaa atatgtatca gattaagtcg aaattgacac atataatcat tccatggagc
     3061 tatgcgcgaa tctaggctga ctcgttcttt catatgtctc catcggcgca tgacaagtaa
     3121 atgaatagaa ttaacatttg acacataaga ttttaattgt taaatttggg caaaataaga
     3181 ccataattgg aattaaattc cattaatatg gttcatccac ttaattatgg gaaatctagc
     3241 catgaaacta gaactaaact ttttgcaaaa acaaacaaaa gaaataatcg ctgaactaca
     3301 aaaactaaaa gactccggag ttacccatct tgatttgagt gggaatgagt ttgataaaag
     3361 gacaggcatc gaattggccg caatattgag tgccctcaaa aattctggag ttactcatct
     3421 tgatttaagt ttaaataatt tcgataaaat gccagcaaca gatttggttg ttatgctgag
     3481 cgctcttaaa ggttctggaa tcactcatct tgatctaagc gggaatgact ttggagaaaa
     3541 atctggggct gaattggttg aaattctgaa tgcaatcaaa ggttctggaa tcacatcact
     3601 tagtttggct gagaatgacc ttgggacaaa gacagaatca gagttattcg atatattgag
     3661 tactcttaag gattctggag taactcatct tgatttgtat ggtaataaac ttggtgaaag
     3721 gaagggacct gagttatcaa ctatgttggg tgctcttaaa gactctggga tcactcatct
     3781 tgatttgagt tggaataact ttggaaaaat ggcaaaaacc gatctgtttg ttatgttgaa
     3841 agcagtgaat catacaggaa ttacccatct taatttaagc gataatttcc ttggcacgaa
     3901 aagcgggaaa gagttatctg agttatttag gcaaatcgaa ggtaaattaa ccattacaat
     3961 tgcgaaaaat aagttggaaa agtgcggttg cagtgccaag gagtttgcta gactgctatt
     4021 tgataccagt catcattatg ttttgggaag cagtgaatat gacaaagcgg tatcagaaga
     4081 gtttcatcga cttaagaagc agcacttggt agctttgtta tttacaggca aaaaactggc
     4141 agagaaaaaa gaaagctttt tttattacga ggggggaagg cgtgatgtaa tgtcattaat
     4201 ctctgccaag atgccatcaa ttcaaataat ttgaatcagt tattattaag aaagtctatg
     4261 agtaattaaa cagacgtttt gtagaaattc atccaaaaat tgatgctgtt cgggt
//
LOCUS       DACTJE010000210         4178 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.212, whole genome shotgun sequence.
ACCESSION   DACTJE010000210 DACTJE010000000
VERSION     DACTJE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.212"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(151..537)
                     /gene="mobC"
                     /locus_tag="I9760_002786"
     CDS             complement(151..537)
                     /gene="mobC"
                     /locus_tag="I9760_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAT5044530.1"
                     /translation="MNTDESKRRRRGPAPLDATDKRGHTVSVRLNDAELARLDSQRDA
                     VQMQRGEYLRAAALHRLPPTIPEVNREAWAALARTAANLNQIAHRLNAGDALPLAEVR
                     ATLDAFRRDLIGVKHDAEDVEDESEG"
     gene            994..1461
                     /locus_tag="I9760_002787"
     CDS             994..1461
                     /locus_tag="I9760_002787"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044531.1"
                     /translation="MQFKRQGRRVQVLAYRGYDKEKRRAIVKMMGSIDAYSYEPSDGL
                     IENLTDEEKTELQSYIKTERQVAEKRSRLYSAKSAASRIKEVADTIKAGDFEPSEAWA
                     ADTWAAIEALTKAMRKAGYPKPRKAPQKAADAPMPGQAGLPLGDAPETPGNAS"
     gene            1992..3287
                     /locus_tag="I9760_002788"
     CDS             1992..3287
                     /locus_tag="I9760_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAT5044532.1"
                     /translation="MGIAVEALFTSALGLQPPWVVDDVRLDTAKRRIDFEIGCHTSRL
                     ACPACGAATQPVHDRLRRSWRHLDFFQFEAWLHCDVPRVACGACGKTTQVAVPWARPG
                     SGFTAAFEALALTLCLDLPVRQAAELLRCKDKRLWRRIEFYVAQARALEDFAGVRTVG
                     IDETSLRRGQHYITVVHDLDRKRLLFATEGREHRTVVEFAEDLKAHGGDPAQVRHVCM
                     DMSAAYAKGVALALPEAQISYDRFHVVSMAIEAMDQVRRAEMATDAQAVRAALGAGGR
                     KTLRQLLWGMRRNPSSWSARQIQAMHWLQRSTLKSARAWRLKMALREVYARATAHNSI
                     EQAASDLRAWLSWARRCRLEPFKKLAATLKERFDAVVRGMVDHRSNAFVEAMNGLLQQ
                     AKRAARGFRTSQNFIAIAYLRMSKLKHLPASPFAPAMPQ"
     gene            3663..>4178
                     /locus_tag="I9760_002789"
     CDS             3663..>4178
                     /locus_tag="I9760_002789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044533.1"
                     /translation="MRPKLPTIILAALLAGTIAIVLNIFVLDALKNAGVETLGGGLQK
                     LMRAKLSGIVLSTGLADAWASSGLPGPDAPSFKTGFKVTVGLLMALIYALLFEPLLRG
                     SSFRKGLITAVPFWLLNAFVVLPLLGHGIAGTRLLTILGLLSYAFAHTLFFVIVALVY
                     GELSRRFKSRHA"
BASE COUNT          794 a         1329 c         1339 g          716 t
ORIGIN      
        1 aactggcgca cggctgagaa ctcccgcgac agttcgcggg ggtcattccc ggccatgttg
       61 ccgccgactc gctccgcgtt tttcgtgtgc gtggcttcct tgccaacgtc aaaaacgtag
      121 ttcagcgcac cgcgaaaacc gccaccacgg ctaaccttcg ctttcatctt ccacgtcctc
      181 ggcgtcgtgc ttgacgccta tcaggtcacg cctgaaggcg tcgagcgttg cccgcacctc
      241 ggccagcggg agcgcgtcgc cagcgttcag gcggtgcgcg atctggttca ggttggccgc
      301 cgtccgggcc aacgcggccc acgcttcccg gttcacttcc gggatagtgg gcggcaggcg
      361 atgcagggcg gcggcgcgta ggtactcacc gcgctgcatc tgcaccgcgt cacgctgcga
      421 atccagccgc gcaagctcgg catcgttgag gcgcaccgag acggtatgcc cgcgcttgtc
      481 cgtggcgtcg agcggcgcag gcccacggcg gcggcgcttg gattcgtcag tgttcattgg
      541 cgaagccctc cagcaaatga agcgaagcga aacgcgcagc aaccccccga ccgaagggag
      601 cgggggcgta ggccgaaggc cggagggtga gaaccaagcg aagcgtaggt tacaccttgc
      661 tccatgccaa agcaccgcgc aaacaccata atagcacatc tccatgcaag caccgaacag
      721 ccacccaccc ggcccgctcg ctgcgctccc ggcccgaccg cccaagggcg gtaatgggcc
      781 agcggggaga ggggtgggca aagcgccggg gcgaactgat accatgccgc tatggcagcg
      841 taagcacggc agactgtcaa gtattactta atccgacagt gcaaacttat ttaagtgcga
      901 cagtcggatt gaaacagaag ccgacaataa acacttaaca gcgtgcgact gtcagactaa
      961 aatacaatca cacagtcaaa cggagaaacc gccatgcagt ttaagcgcca aggtcgccgg
     1021 gttcaggtgc tcgcctaccg ggggtatgac aaggaaaagc gccgcgccat cgtcaagatg
     1081 atggggtcta ttgacgcata ttcttacgag ccgtcagacg gcctaattga gaatctgaca
     1141 gacgaagaaa aaacagaact acagtcatac attaaaacag aacgacaggt ggcagaaaag
     1201 cgaagtcgcc tgtattccgc taagtctgcc gccagtcgga ttaaagaggt tgccgacaca
     1261 atcaaggccg gggactttga gccgtccgag gcatgggcgg cggatacctg ggcggcaatc
     1321 gaggcgctga ccaaggccat gcgcaaggcg ggctatccga agccccgcaa agcgccccag
     1381 aaggccgctg acgcgccgat gccgggtcag gcagggctgc ccttggggga tgcgccagaa
     1441 acgccgggaa acgcttcctg aggggtttag gccgggaaac cggccccacc cctctccccg
     1501 ctggcccttt acccccggag ggggtcgccg aaggcggggg gagaggtgtt cgccttccgg
     1561 ccttggccgg gcattttggg caatgccaga ccgcgccgaa ggggcgcggg ctgtgctgcg
     1621 cctagtggag attcttggag aatcttggat ggattttgcg aatcgccagc aaagccgcgc
     1681 cccgtctggg ttttccagat cggggcgggt ggagttaccg tgggggttgg gtggagttac
     1741 cgtgggggtt gggtggagtt accgtggggg tcacacatac tcgcaccaga tcacgctgcc
     1801 ccagtgctcg ccggtttccg gctcaatggc tacgctatcg aagcgccctc ctgagtaggt
     1861 ccttcgtcat ttcgtgtgga acccaacagt cacaatgact cctggtcgtc gagcccggtg
     1921 ggcatgtgtg caggccgctg gccggcctgt gcacatgtcc acgaggcgtg tttgatcagg
     1981 aggatgagtc catgggcatt gccgtagaag ctctgttcac cagcgcgctg ggcttgcagc
     2041 cgccgtgggt cgtcgatgac gtcaggctcg acaccgccaa gcggcgtatc gacttcgaga
     2101 tcggctgcca caccagcagg ctcgcctgcc cggcatgcgg tgcggccacg caaccggtgc
     2161 acgaccggct gcgccgatcc tggcggcacc tggacttctt ccagttcgag gcctggctgc
     2221 actgcgacgt gccgcgcgtg gcctgcggtg cctgcggcaa gaccacgcag gtggccgtgc
     2281 cctgggcgcg tccgggctcg ggcttcaccg cggcgttcga agcgctggcg ctgaccttgt
     2341 gcctggacct gccggtgcgc caggccgccg agttgctgcg ctgcaaggac aagcggctgt
     2401 ggcggcgcat cgagttctac gtcgcgcagg cgcgtgcgct ggaagacttc gccggcgtgc
     2461 gcacggtggg catcgacgag accagcctgc ggcgcgggca gcactacatc accgtcgtgc
     2521 acgacctgga tcgcaagcgg ctgctgttcg ccaccgaggg gcgcgagcac cgcacggtgg
     2581 tggagttcgc cgaggatctg aaggcccatg gcggcgatcc cgcccaggtg cggcacgtgt
     2641 gcatggacat gagcgcagcc tacgccaagg gcgtggcgct ggcgctgccc gaggcgcaga
     2701 tcagctacga ccgctttcac gtcgtctcga tggccatcga ggcgatggac caggtgcgcc
     2761 gcgcggagat ggccaccgac gcgcaggcgg tgcgagccgc gctgggcgct ggcggccgca
     2821 agacgctcag gcagctgctg tggggcatgc ggcgcaaccc cagcagctgg agcgcccgcc
     2881 agatccaggc gatgcactgg ctgcagcgct cgacgctcaa aagcgcgcgg gcgtggcggc
     2941 tgaagatggc gctgcgcgag gtgtacgcac gggccacagc gcacaacagc atcgaacagg
     3001 ccgcatccga cctcagggcc tggctgagct gggcgcggcg ctgtcggctc gagccgttca
     3061 agaagctggc cgccacgctc aaggagcggt tcgacgcggt ggtgcgcggc atggtcgatc
     3121 accgcagcaa cgccttcgtc gaggcgatga acgggctgct gcagcaggcc aagcgcgccg
     3181 cgcgcggctt caggacgagc cagaacttca tcgccatcgc ctacctgcgc atgtccaagc
     3241 tcaagcacct gccggccagt ccgttcgcgc cggcgatgcc ccagtgacag tgttgctttc
     3301 cacacgaaac gacatggagc cgctgtggca cgccctggcc cacaacctca tgcgcatgcg
     3361 cagcctgcaa atcgccttca aggcctgagc gacgacggcc cgcagcgggc gcacaagaca
     3421 tcacaccaac gccccctcgc gcctcacagc ccctgacagc cccacccaga caagcttcag
     3481 acgaagtgcg catcacgtcc gcccgctgcg ccttcgacgg cgcatccaac ggaacatcag
     3541 aaatggcgag tggttcgttc acagcttctc agcccaacag gacctcgata taatagtcgc
     3601 gtgactcaaa ccaaaccgcc tccggaaaac ccgggacgct tcaaaccgaa tggaaactcg
     3661 ttatgcgacc aaaactgccg acgattattc tggccgcatt gcttgccggc accattgcca
     3721 tagtgctcaa catattcgtg ctcgacgccc tgaagaacgc cggtgtcgaa acgctcggcg
     3781 gcggattgca gaagctgatg cgtgccaagc tgtccggaat agttctatcg accggactcg
     3841 ctgacgcgtg ggcttcgagc ggacttccag gcccggacgc tccctccttc aaaactggtt
     3901 tcaaagtcac ggtcggactc ttgatggccc ttatctatgc gctgttgttt gaaccgctgt
     3961 tgcgcggctc ctcgtttcgc aagggcctga ttacggcggt gccgttctgg ttgttgaacg
     4021 cattcgtcgt actgcctctg ctcgggcatg gaattgcggg tacgcgcctc ctgacgatcc
     4081 tgggcctctt atcatacgcg tttgcgcaca cgctgttctt cgtcattgtt gccttagtgt
     4141 acggtgaact gtcgcgccga ttcaaaagcc gccatgcc
//
LOCUS       DACTJE010000211         4158 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.213, whole genome shotgun sequence.
ACCESSION   DACTJE010000211 DACTJE010000000
VERSION     DACTJE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.213"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            106..2103
                     /locus_tag="I9760_002790"
     CDS             106..2103
                     /locus_tag="I9760_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HAT5044534.1"
                     /translation="MLNSQQMAAVKYIDGPLLVLAGAGSGKTRVITQKIGYLINSCGY
                     NANSICAVTFTNKAANEMRARVSAFLPAVSRRGLKVATFHTLGLSIIKRDVSRCDLKP
                     GFSIFDSEDCLQILRGFLPVNKATDRDFILKVQQQISLWKNNLLNPELILQNPPETLL
                     CDEALTIYPRYQEALKVYNAVDFDDLIRLPVDLLNDHADVLEYWQNKIRHLLVDEYQD
                     SNTSQYLLVKKLAGVRAHFTVVGDDDQSIYAWRGAKPENLAQLQKDFPRLKIIKLEQN
                     YRSTSRILHAANHLIAHNQHLFEKKLWSDLGHGELLRVVSCKDEQDEAEHVIADLISH
                     KLRNRTNYSDYAILYRGNHQARIFEKVLRHHGIPYHISGGQSWFAKLEVRDIFAYLKL
                     LCNEADDAAFLRAITTPKRGIGESSLDALGRYAQSRGISLYHGSDHLALSEFVADKQR
                     MIFHDFKLWMEDIKKRISSDSVLEHLRQMVEDIGYEAYIYEQCDSPAKAQKKMDNVWE
                     LLEWVNRLLNKEPEQSLMDVVNKLILIDILEQSDESSSDVVQLMTLHASKGLEFPFVY
                     LVGMEEELLPHRVSIDDDQIEEERRLAYVGITRAQKGLCFTLAKQRRRAGELQDCVPS
                     RFLDELPQESLEWFGKGGERCEEQSKKLAKSHLEGLKNLLS"
     gene            complement(2187..4154)
                     /gene="iroT"
                     /locus_tag="I9760_002791"
     CDS             complement(2187..4154)
                     /gene="iroT"
                     /locus_tag="I9760_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter IroT"
                     /protein_id="HAT5044535.1"
                     /translation="MPIKPQKIPYYLFLLLLTVGASLILGFLSFGGMYALWPVLPLAF
                     AGFALSVAYEGEIYLQNIKGALNKLFKFNYLKNYLAKEYLLNHFPNTDEENCPQFFKD
                     YKKQLTLLAEFGHKELNQDSKKRKRQIEKTLSDMEKWFALQLFSDKNNPSPNTSKYTI
                     ELLDWLALNKRDEWIKQYEKRHFQFHIVKGFSVVAGLFMGLGSTYLIVEAFTIIPFFA
                     TIPFTLWPIIILPMATVAGAAYGMLTYNAVTDMINNNTVVKWYNKIRNDLSQGLTVRN
                     VFIATTAVFLVGLALALTVCTAGTWWTIATNARPLFDWMKKMPSFIMGIINPIITGAS
                     AIVFNIQNTAESLDMVDEATRSNKNVFQKIYEAISNGYQHLRETENWLQIVNPFRILL
                     KLTITPLRILLFLGHLISVAVTSDRMPGVPQILSALVAIISEGFEDAHYFIGHTHEDE
                     LDEEHGHQDHHQFEKLLKERLDPDSDQDHNMDIPTWILKTIASPIYGLAALWDCSASK
                     LNPSQENHSKNLPSGKKPHVLSLGEAWNKQWGVAKEFNVEFSTHAKRPSQEWQVEHAV
                     AQIDKFQRKHLKDIVIGRELADKKIIALNHLKEKIRHPEKGETLSDTLEQAKKQPDYN
                     QHRLFNQKGEKTRTQLFIEELPERINLSSGK"
BASE COUNT         1238 a          711 c          911 g         1298 t
ORIGIN      
        1 tttgtgacaa tgaagacgag tttgatgtag aacgctctat caattaattc ttgcgtgaaa
       61 aagaaagcct gggtgataat gccttttttt tgagcgggaa taagtatgtt gaatagccaa
      121 caaatggctg cagtaaaata tattgatggg cctttgttgg tgcttgccgg ggctgggagt
      181 ggtaaaacca gagttattac acaaaaaatt ggttatttaa ttaattcatg tggttataat
      241 gccaactcca tctgcgccgt tactttcacg aataaagcgg cgaatgaaat gcgcgcaagg
      301 gtttcggcct ttttaccagc tgtaagcagg cggggattaa aggttgcgac atttcataca
      361 ttaggcctaa gcatcatcaa gcgtgatgtt tctagatgcg atttgaaacc aggattttcc
      421 atttttgata gtgaagattg tttgcaaatt ttgcggggtt ttttacctgt taataaggca
      481 accgatagag attttatcct caaagttcaa caacaaattt ctctttggaa aaataattta
      541 ttgaatcccg aattaatact ccaaaaccct ccggaaacac ttttatgtga cgaggcgtta
      601 acaatttatc cacgctatca agaagcatta aaagtatata atgcagtgga ctttgatgat
      661 ttaattcggt tacctgtcga tctactgaat gatcatgctg atgtgttgga atattggcag
      721 aataaaattc gtcatttgtt ggtggatgaa tatcaggatt caaacactag ccagtatctt
      781 ctggtaaaaa aattggcagg ggttcgtgcc cactttacgg ttgtgggtga tgatgatcaa
      841 tcgatttatg cctggcgtgg tgccaaacca gagaatttgg cgcagttaca aaaagatttt
      901 cctcggttaa agattattaa attagaacag aattacagat caaccagccg aattttacat
      961 gctgccaacc atctgatcgc tcataatcaa catttatttg aaaaaaaatt atggagtgat
     1021 ttggggcatg gggaattatt gagggttgtc agctgtaagg atgagcagga tgaggcggaa
     1081 catgtgatcg ctgatttaat aagccataaa ttacgtaatc ggacaaatta tagtgattac
     1141 gctatcttat acagaggaaa tcatcaagca agaatatttg aaaaggtatt aagacatcac
     1201 ggtattcctt atcatatcag cggaggccag tcctggtttg ccaagttaga agtcagggac
     1261 atttttgcct atcttaaatt actttgtaat gaagcagatg atgccgcttt tttgcgagca
     1321 attactaccc ccaaacgagg tataggagag agcagtctcg atgccttggg acgctatgct
     1381 cagtccagag ggataagttt atatcacgga tccgatcatt tggccttgtc tgagtttgtt
     1441 gctgacaagc agagaatgat ttttcatgat ttcaaattgt ggatggaaga cattaaaaaa
     1501 cgtatcagtt cagattcagt tctggagcat ttaaggcaaa tggtggaaga tattgggtat
     1561 gaagcttata tttatgaaca atgtgattct cccgccaagg cacaaaaaaa aatggataat
     1621 gtatgggagc ttttagaatg ggttaatcgt ttattgaata aggaacctga acaatcgctc
     1681 atggatgttg tgaataaatt aatactaatc gatatactgg aacaatctga tgaatcttca
     1741 agtgatgtag tccaattaat gacattgcat gcctcaaaag gtttggaatt tccttttgtt
     1801 tatttggtcg ggatggaaga agaattatta ccccaccgag tgagtatcga tgatgaccaa
     1861 attgaagaag aaagaagatt ggcttatgta gggatcacac gtgcccagaa gggattatgt
     1921 tttactttag cgaagcagag acgtcgagct ggagaactgc aagactgtgt tccaagccga
     1981 tttttggatg aattgccgca agagagtctg gaatggttcg gtaaaggcgg ggagcgttgt
     2041 gaagaacaat caaaaaaact ggccaaatcc cacctggaag gactgaaaaa cttgttgagt
     2101 taagagtaca ttatatttta tctccagtaa ggagtatcat taactgaact ccttactgct
     2161 ttttaaggta ctaatgttaa tctctatcat ttgccagaag agagattgat tcgttctggg
     2221 agttcttcaa tgaaaagttg tgtacgggtt ttttcccctt tttggttaaa tagtctgtgt
     2281 tgattgtagt ctggttgctt tttagcttgc tccagagtgt cactcaatgt ttctcctttt
     2341 tcaggatggc gtattttttc ttttaaatga ttgagggcga tgattttttt atcggctaat
     2401 tcgcgaccga taacgatgtc tttgagatgc tttctttgaa atttgtcgat ttgggcaaca
     2461 gcatgttcca cttgccattc ttgcgatggg cgtttggcat gagtactaaa ttcaacattg
     2521 aactctttgg ctactcccca ttgtttattc catgcttctc caagacttaa cacatgaggt
     2581 tttttgccag aaggcaaatt cttactatga ttctcttgag aaggatttag cttactggca
     2641 gaacaatccc agagtgctgc caagccatat ataggtgaag caatggtttt taatatccag
     2701 gtaggaatat ccatattatg atcttgatcg gagtcaggat ctagtctttc ctttaggagt
     2761 ttttcaaatt ggtgatgatc ttggtgtcca tgttcttcat ccagttcgtc ttcatgggta
     2821 tgtccaataa aatagtgggc atcttcaaag ccttcgctaa taatggcaac gagcgcggaa
     2881 agaatctgcg gtactccagg catacgatcc gatgtgactg caacgctgat caaatgcccc
     2941 agaaacaaaa gaatacgcaa tggggtaatg gttaatttta aaagaatgcg aaacgggtta
     3001 actatttgta accaattttc agtttcacgt aagtgctgat atccattgct tattgcttcg
     3061 taaatttttt ggaagacatt cttattacta cgagttgctt catcgaccat atccagtgat
     3121 tcagccgtgt tttgtatgtt aaagactata gcagaggcgc cggttataat gggattgata
     3181 attcccataa taaaactggg catttttttc atccagtcaa ataaagggcg ggcatttgta
     3241 gcaatcgtcc accatgttcc agcagtacag acggttaggg ctagtgccag tccaacaaga
     3301 aaaacagcag tagttgctat aaacacattg cgtacagtta aaccttggct taaatcattg
     3361 cgaattttat tataccattt gacaactgtg ttattgttga tcatgtcggt aacagcatta
     3421 taggtcagca ttccataagc agcccctgcg acggtggcca tgggaagaat tataatgggc
     3481 caaagtgtga atggaatggt ggcaaaaaac gggatgatcg taaaggcttc gacaatcaga
     3541 taggtacttc ccagccccat gaaaaggccg gcaaccacgc taaaaccttt gacaatatga
     3601 aattgaaaat gacgcttttc atattgtttt atccattcgt ctcgcttatt taaagcaagc
     3661 cagtcaagta gctctatggt gtatttggat gtgtttggag aaggattgtt tttatctgaa
     3721 aacaattgca gggcaaacca cttttccatg tcggagagtg ttttttcaat ttgacgtttt
     3781 cttttcttac tgtcttgatt gagttcttta tgcccaaatt cagccaataa tgttaactgt
     3841 tttttataat ctttgaaaaa ttgcgggcaa ttttcttcgt cggtgtttgg aaaatgattc
     3901 agcaaatact ctttggctag gtaattctta agatagttaa atttaaacag tttattaaga
     3961 gcgcctttta tgttttgtaa atagatttca ccttcataag ctaccgataa cgcaaaacca
     4021 gcaaaagcca aaggtaacac aggccaaaga gcgtacatgc ctccaaagct taaaaagcca
     4081 agtatcaggc ttgctccgac agttaatagc aaaagaaaca agtaataagg gattttttgg
     4141 ggcttaatag gtatattt
//
LOCUS       DACTJE010000212         4154 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.214, whole genome shotgun sequence.
ACCESSION   DACTJE010000212 DACTJE010000000
VERSION     DACTJE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.214"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..771)
                     /locus_tag="I9760_002792"
     CDS             complement(<1..771)
                     /locus_tag="I9760_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HAT5044536.1"
                     /translation="MPTRVIEDKGDMTPSFGIDDRIFLGEGLFETIRVNSSKPSFAYM
                     HWERLGNSARQLGIPFEISFDDWFEHLIQKIQKDNLYHGGIKAILSGGPASRGLAERG
                     QVSQLIFQTFNYSIQKHPVRLISINWLRDKANPLYQLKSVNYLEAIIAQRQAIAVGAD
                     DALFFNTENHVTETTCANLFLIENNILYTPRVDDGILPGITRARLISHCQQHKMSVQE
                     ISLTKKRIEDADAVFLTNSLQGIRRVLSLDNIIFEVNHP"
     gene            complement(785..970)
                     /locus_tag="I9760_002793"
     CDS             complement(785..970)
                     /locus_tag="I9760_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044537.1"
                     /translation="MSDLFEEDEEEVIEVVDDFDDVEVDTEIIEAPGVSLDARRRLEN
                     MLEEKRLRDELDDFVDY"
     gene            1217..1798
                     /locus_tag="I9760_002794"
     CDS             1217..1798
                     /locus_tag="I9760_002794"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAT5044538.1"
                     /translation="MSDQYKVALRNTIKQVRSKLSVSYRTASSNQICTRIRSLEQYRQ
                     AKRIALYFAVNGEIDLTFLWKSAPLQGKYCYFPVLNNDSTLSFLPATPSTPFKTNKYD
                     IPEPDVDIELALPVDELDLIIMPLVAFDAHCTRLGMGSGYYDRTLANKSKAHLLGVAY
                     QFQRVDYIEPRPWDVPLDAVITQKAIYWRNPLA"
     gene            complement(1808..2146)
                     /locus_tag="I9760_002795"
     CDS             complement(1808..2146)
                     /locus_tag="I9760_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAT5044539.1"
                     /translation="MKMITAIIKPFKLDDVHEALMEIGVPGITISETRGFGRQKGHTE
                     LYRGAEYVVDFLPKIKIELALPDNMVEAAIEAICKSAYTGKIGDGKIFVYALEQVVRV
                     RTGEVGTDAL"
     gene            2291..2551
                     /locus_tag="I9760_002796"
     CDS             2291..2551
                     /locus_tag="I9760_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HAT5044540.1"
                     /translation="MFDPKQFDELANKLFATLPTSLQNFEKDIQQKFKEVLQSTFSRM
                     DLVTREEFDVQCKVLARTREKLEQLQHQLEDFIKQQEIKNNK"
     gene            2616..4127
                     /locus_tag="I9760_002797"
     CDS             2616..4127
                     /locus_tag="I9760_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAT5044541.1"
                     /translation="MSLAFTKTRSTIGIVAQPVSVEVHLSNGLPSFTMVGLAETAVKE
                     SKDRVRSAIINSQFEFPCRKITVNLGPANLPKTGSGFDLPIALGILAASEQIPLTNLA
                     NHEFIGELALSGELRGVSAIIPAVLAAHKDNQHLIIANANAAEASLTGHQKVFTANNL
                     REVCDYLCQGTSLQSLPPKPDLLLNNYELDWSDIKGQQHAKNAMVIAACGGHSILLSG
                     APGSGKTMMAKRFSTLLPELSETQALECAAINSIRGKLPDFREWRLPPFRAPHHTASP
                     VALVGGGNPPKPGEISLAHHGVLFLDELPEFNRQVLETLREPLESGHICISRAAAQIE
                     FPAKFQLIAAMNPCPCGQWGNSQANCMCTPDRISRYLAKLSAPLLDRIDMQVTIHALS
                     QEELIKPNTHLEKQSLAIREKVTKMHEIQMARQDSLNANLNSKTCEMVCELGSEEQLF
                     LREVMSKLKLSARGYHRLLKVSRTIADMNSSKKVLLNHLQQALSYKQNLHLPK"
BASE COUNT         1317 a          902 c          725 g         1210 t
ORIGIN      
        1 cggatgattg acttcaaaaa taatgttatc cagggagagc acccttctga taccctgcag
       61 actattagtt aaaaaaacag catcggcgtc ctcaatcctc tttttcgtta atgatatttc
      121 ctgcactgac attttgtgtt gctgacaatg tgaaatgagg cgtgccctgg taatgccggg
      181 taagatccca tcatctactc gcggggtata caaaatgtta ttttcaatca agaataaatt
      241 ggcacaagtt gtttcagtaa catgattctc tgtgttgaaa aataaggcat cgtctgcccc
      301 aaccgcaatc gcttgacgtt gagcaataat cgcctctaaa taattcactg attttaattg
      361 ataaagagga ttggccttat cccttagcca attaatactg attaaccgaa caggatgctt
      421 ttgtattgaa taattaaaag tctgaaatat taattggctg acttgtcctc gttctgccaa
      481 gcctctcgaa gcagggccac cactcaaaat ggctttaatg ccgccatggt acaggttatc
      541 tttttggatt ttttgaataa gatgctcaaa ccaatcatca aatgaaattt caaaaggaat
      601 acccaactga cgagctgaat tgcctagtct ctcccaatgc atataagcaa aggaaggctt
      661 ggaactattg actctaattg tttcaaataa tccctcacct aaaaaaattc gatcatcaat
      721 tccaaatgat ggcgtcatat ctcctttgtc ttcaataaca cgagtaggca ttatgatctc
      781 ataattaata atctacaaaa tcatccaact catctcgcaa tcgcttttcc tccaacatat
      841 tttccagtcg tctgcgcgca tccaaactca caccgggagc ttctataatt tcagtatcca
      901 cttcaacatc atcaaaatcg tcgacaactt caattacttc ttcttcatct tcttcaaaca
      961 agtcactcat aacattccct caaagaatta aaagtaacat taaataattg cttaaatgga
     1021 acaagattcc aggacggcta tataccttat ttttaatttt ttgcatagta ttttttaatt
     1081 attttatgtt ataaacttat cgcatcgcgc atttataatc acccaatgca taaaacaaca
     1141 tattgatacc tggaaacact tgaactttaa gctcaatcca tctaataata aaaaagaata
     1201 ttagtgatac caaatcatgt cagaccaata taaagttgct ctaagaaata ccataaaaca
     1261 ggtaagatcc aagctttctg tatcctacag aacagcatct tccaatcaaa tttgtactcg
     1321 tatccgctcg ctggaacaat acaggcaagc aaagcgaata gccttgtact ttgcagttaa
     1381 tggagaaatt gatttaacat ttctctggaa aagcgcgccc ctccagggga agtattgtta
     1441 ttttccggta ttaaataatg attccactct ttctttttta ccagccaccc ccagcacccc
     1501 ttttaaaacg aataaatacg atatcccgga accggacgtt gatattgagt tggccttgcc
     1561 tgttgatgaa ttagacttga ttatcatgcc tctggttgca tttgatgcac actgtactcg
     1621 tttgggtatg ggctcaggct actatgatcg caccttggct aataaatcaa aagcgcatct
     1681 cttaggagtc gcctaccaat ttcaaagggt tgattacata gaaccacgcc cttgggatgt
     1741 tcccctggat gccgtaataa ctcaaaaagc tatttattgg cgaaatccct tggcctaaaa
     1801 tgacattcta tagagcgtct gtaccgacct ccccggttct aaccctcact acctgttcca
     1861 aagcataaac aaaaatcttg ccatcaccaa ttttaccggt gtaggcggac ttgcaaatag
     1921 cttctatggc tgcttcaacc atattatctg gcaatgccag ctcaatttta attttaggga
     1981 ggaaatctac cacgtactcc gcacctcgat ataattcggt atgacctttt tgacgcccaa
     2041 atcctcgagt ttcagatatg gtaattcctg gcactcctat ttccattaaa gcttcatgta
     2101 catcatccag tttaaatggt ttgataatcg cagtgatcat tttcatattt ttttccatca
     2161 cgttaaatgc agagccaccc atgatatttc taacacacaa tttttatgtt attgcttttc
     2221 aagacatctt gggtatagtt gtaatccaac agttttgcta gactgtattc aaaaataatc
     2281 cggagccacc atgtttgacc ccaagcaatt tgatgaatta gctaataagc ttttcgctac
     2341 tttacctacg agtctacaaa attttgaaaa agacattcaa caaaaattta aagaagtact
     2401 gcaatcaaca ttcagccgaa tggaccttgt tacccgtgaa gaatttgatg tgcaatgtaa
     2461 agtacttgcc agaactcgag aaaaactgga gcaattgcag catcagcttg aagacttcat
     2521 taaacagcaa gaaattaaaa ataataaata ataactatta gtcgtccata ggaaattgga
     2581 tcgatttaag tccaacataa taaggaaaaa atcttatgag tctcgcattt accaaaacgc
     2641 gaagtactat tggtattgtc gcacaaccag tatctgtgga agtccattta tcaaatggat
     2701 tgccaagttt tactatggta ggacttgctg aaacagcagt gaaagaaagt aaagaccgcg
     2761 tgcgcagcgc cattataaat agccaattcg aatttccatg ccgtaaaatt accgttaatc
     2821 ttggccctgc caatttacct aaaacgggaa gtggctttga cttgcctatt gcactaggga
     2881 tactcgcagc atcagaacaa attcctctca ccaaccttgc aaaccacgaa tttattggag
     2941 aattggcttt aagcggcgag cttagaggag tttctgccat tataccggct gttcttgccg
     3001 ctcataaaga caatcaacat ctgattatag cgaatgccaa tgctgccgaa gcttctttaa
     3061 ccggccatca aaaagtcttt actgccaata acttacgtga agtttgtgat tatctatgcc
     3121 agggcacttc tttacaaagc ttacctccca aaccagatct tttattaaat aactatgaat
     3181 tggattggtc tgatattaag ggacaacagc atgccaaaaa tgctatggtc atcgctgcct
     3241 gtgggggaca cagcattctg ctaagtggcg caccaggaag tggaaaaacg atgatggcaa
     3301 aaagatttag caccttgcta cctgagctat ccgaaacaca agctctcgaa tgcgcagcca
     3361 ttaattcaat tcgaggcaaa ttacctgatt tcagggaatg gcgtttgcct ccttttcgtg
     3421 caccacatca tactgcttca cctgtcgcgt tagtcggagg aggcaacccg ccgaaacctg
     3481 gtgaaatttc attagcccat cacggcgtat tatttttaga tgaattacct gaatttaatc
     3541 ggcaagtatt ggagacactt agagaacctt tggagtcagg gcatatttgc atttccaggg
     3601 cagccgcaca aatagagttt cctgccaaat ttcaattaat tgcagcaatg aatccttgcc
     3661 cttgtggcca atggggtaat tcacaagcca attgcatgtg tactcctgat agaatcagcc
     3721 gctatttggc aaaactttct gctccattat tagatagaat cgatatgcag gttacaattc
     3781 acgcattatc tcaagaggag ttaattaaac ccaataccca tttagaaaaa caaagtctcg
     3841 ctatcagaga aaaggtaaca aagatgcatg aaatacaaat ggcacgacaa gatagtctca
     3901 atgccaattt aaattccaaa acatgtgaaa tggtttgcga attgggatca gaagaacaat
     3961 tgtttctccg agaagtgatg agtaaactga aactgtctgc tcgtggctac catcgcttac
     4021 taaaagtaag caggacaatt gctgatatga atagcagtaa gaaagttctg ttaaatcact
     4081 tgcaacaagc tttatcttac aaacaaaatt tacacttacc gaaatgacac tgattataaa
     4141 gataacccgt acag
//
LOCUS       DACTJE010000213         4075 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.215, whole genome shotgun sequence.
ACCESSION   DACTJE010000213 DACTJE010000000
VERSION     DACTJE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.215"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(140..859)
                     /locus_tag="I9760_002798"
     CDS             complement(140..859)
                     /locus_tag="I9760_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HAT5044542.1"
                     /translation="MVDYHYFKSKLNHFYGRYVGYNQRPVFFDIDQTFPELNHITRHF
                     PDIKREFENVQANVKEFPRYHDIDPGESAISNTTEKNWNVFMLYLLGYQLKEARLLCP
                     ELCNLLEGIPHLIQAFFSILEPGKSIPLHEGPYIGYLRYHLGIHVPQNNPPQILVNNQ
                     TYTWKEGEAVLFDDSWPHEVRNTSDDYRAVLIIDVLRPMPFLPQLVNQFVTHVIARYF
                     YGRKVMKRAMQYGGEVKPLAL"
     gene            complement(1123..2055)
                     /locus_tag="I9760_002799"
     CDS             complement(1123..2055)
                     /locus_tag="I9760_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5044543.1"
                     /translation="MSKQENIISHNTDSSLLNTVMQQMKERIINEGDKPHVTVETQLK
                     ILQELSQFGFGQFLIKNQGINGYWTHYMLTHPWFGRKTGLNNSREPLSNMECFLLDKA
                     PTMLATQQRFELFLQENQKALVNGATLASIPCGMLGELLYLDFNGINNIQLMGIDYDQ
                     AALEDANALAKERNLDRFTTLVCRNAWELNIQEEFDLISSNGLNIYEPDDNKVLDLYQ
                     QFHTALKKGGKLVTSFLTPPPHLSSQCEWIFNKINPEDLLLQRIIFSDILNAKFQCYR
                     SSQQTQKQLALAGFKNIHFLYDEAHLFPTVIAEK"
     gene            complement(2262..2900)
                     /locus_tag="I9760_002800"
     CDS             complement(2262..2900)
                     /locus_tag="I9760_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2461 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044544.1"
                     /translation="MDNRRKGMSQFNFFEKNQAFDENNFEQKLQDAINLIKQNQSFVA
                     RLVLDAINSNQVTLSTFYQLTPNHYQKTKTIMRKDYHTILPNTFPPTISAVRKIEKEL
                     DGIIYDNQHIYISSTKSVEEIAKTLIHEVCHFLNSDLYDKEMETEESKQVGYKDEVRA
                     FTAEKMFEKNKGYLLRSDINQIHETVTELYPEFTDPENPMSGYIYSTFDSPS"
     gene            3041..3424
                     /locus_tag="I9760_002801"
     CDS             3041..3424
                     /locus_tag="I9760_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HAT5044545.1"
                     /translation="MYIQKLKEMFTQMVIKKNANLIPEYYHGEFLLYTNDIVTGYDEF
                     LSSHQQYYATEIQYQVEYDEETFLEQGEKIAGRVFITTTRPNEPVKRIEVILIAQYKD
                     EKIYRLWELTYPDWSKLPAFSNQLT"
     gene            3650..>4075
                     /locus_tag="I9760_002802"
     CDS             3650..>4075
                     /locus_tag="I9760_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate cyclase"
                     /protein_id="HAT5044546.1"
                     /translation="MKGIIFNEFLNFVEKSESYTLVDQIIMDSHLKSHGAYTSIGTYS
                     PKELFQLVKALAMKNGKPTSVILQEYGEYLFEVFAKKYPQFFREKKSVFQFLEALETH
                     IHFEVKKLYDYTELPHFECQYHSQNQMEMIYTSSRPLADF"
BASE COUNT         1259 a          684 c          738 g         1394 t
ORIGIN      
        1 cttatatcgc tctttttagt atagagaaag actgataaga gctaattcaa caagggttga
       61 acctaaagtt caacttttct ttttttattg attgataaat attttaaacg attttcgaaa
      121 gataataatc gttgtttact taaagtgcca aaggctttac ttctccacca tactgcatcg
      181 cgcgtttcat tactttgcga ccataaaaat atcgagcaat gacgtgggtt acaaattgat
      241 tgaccagttg cggcaagaag ggcatagggc gcaagacatc aatgattaat acggcacgat
      301 aatcatcact ggtatttctt acttcatgtg gccaggagtc atcaaaaagt actgcttctc
      361 cttctttcca ggtataagtt tggttattaa ctaaaatttg tggtggattg ttttggggaa
      421 catgaattcc taaatgatat cttagatagc caatataggg gccttcatgg aggggaatag
      481 attttccagg ttctaaaatc gagaaaaaag cttgtataag atggggtatt ccttccaata
      541 aattacaaag ttccgggcat aataaacgtg cttccttgag ttgataacca agaagataca
      601 gcataaaaac attccagttt ttttctgtag tgtttgatat ggcagactct ccgggatcta
      661 tgtcgtggta gcgtggaaat tcttttacat tcgcttgtac attttcgaat tcccttttga
      721 tatcgggaaa atggcgtgtt atgtgattta attctgggaa agtttgatca atatcaaaaa
      781 aaaccgggcg ttgattatat cctacatagc gcccataaaa gtgattcaat ttactcttga
      841 aataatgata gtctaccatt gcttattcct ttattttatt tgtgataata atcaggtgcc
      901 gaatgttcga ctatccttgg gaaaatcagc tattttaaaa tgaagtttga agaaatggaa
      961 gtttaaaaaa tgctcgtttc tgtgccgagg tatcctttca ggttttggaa atgatttttg
     1021 gcatccagct tattccaagt aagcatattc tcaatggcag tcatcatata atatttatcg
     1081 ctatggctta aaaaaatacg gataacaatt tcctttttct tgctattttt cagctattac
     1141 ggttgggaag agatgagctt catcatatag aaaatgaata ttcttgaagc ctgccaaggc
     1201 gagttgcttc tgggtttgtt gcgatgagcg ataacactgg aattttgcat ttaaaatatc
     1261 ggaaaaaata attctctgca ataataaatc ttctgggtta attttattga atatccattc
     1321 gcattgtgaa ctcaggtgag ggggaggagt taaaaaactg gttactaact tcccgccttt
     1381 ttttaaggct gtatgaaact gctggtataa atccagaact ttgttatcgt ctggttcata
     1441 aatatttaac ccattgctgg aaatgagatc aaattcctct tgaatattaa gctcccaggc
     1501 attacgacaa acaagcgttg tgaatctgtc caggtttcgt tcttttgcca aggcattggc
     1561 atcctctaat gctgcctggt cataatctat acccatcagt tggatgttgt tgatgccatt
     1621 gaaatcaaga taaagtaatt cccctaacat cccacagggg atagaagcaa gggttgcgcc
     1681 attaaccaga gctttttgat tttcctgcaa aaataattcg aaacgctgtt gggttgctaa
     1741 cattgttggt gctttgtcca acaagaaaca ttccatgttt gataatggct cacgactatt
     1801 gtttaatccc gtctttcttc caaaccaagg atgagtcaac atataatgag tccaataacc
     1861 attgataccc tgatttttta ttagaaattg accaaaccca aattggctta attcttgaag
     1921 aatcttaagt tgagtttcta ccgtgacatg tggcttatca ccctcgttta ttattctttc
     1981 cttcatttgc tgcattacag tattcagaag agatgaatcg gtgttatgag aaatgatatt
     2041 ttcttgtttt gacatgcagg ctctctggat aatctttata attattaatt gctatgagtt
     2101 taagataaga aaatggattt attttataat tcaaactcac agcaattata cttaatattc
     2161 ttgaagatac gtagattttt taacttattt cattttatgt tccactacgg cgctcgaaaa
     2221 tcaaggcttt tcgactcaag ctggcaaaat gtaaacagcc cttaagaagg gctatcaaaa
     2281 gtgctgtaaa tataccctga cattgggttt tccggatcgg tgaattcagg atatagttcg
     2341 gtaaccgttt catgtatttg gtttatatcg cttctgagca ggtagccttt atttttttca
     2401 aacatttttt ctgctgtaaa tgctcttact tcatctttat agccgacttg ttttgactct
     2461 tctgtttcca tttctttatc gtaaagatct gaatttaaaa aatgacagac ttcatggatt
     2521 aatgttttgg caatttcttc aacgcttttt gtcgaactga tataaatatg ttgattatca
     2581 taaataatgc catccagttc tttctcaatt ttacgtactg cggaaattgt aggggggaaa
     2641 gtatttggaa ggatagtatg atagtccttt ctcataattg ttttcgtttt ctggtaatga
     2701 tttggggtaa gttgatagaa agtacttaat gtgacttgat tggaatttat cgcatcgagc
     2761 actaatcttg caacaaaaga ttgattttgt tttatcagat tgattgcatc ctgcaatttt
     2821 tgttcaaaat tgttttcatc aaaagcctgg tttttttcaa aaaaattgaa ttgagacatt
     2881 ccttttctcc tattatccag tattaatgat tacttccatg caatttcata gtaacctttc
     2941 ttcaattaat gaacaatact gagggtaatt tttcaaaatg atcagtttta ttgctaaata
     3001 attgttgtat aattttgaaa aattaatttg agagtagaaa atgtatatac aaaaattaaa
     3061 agaaatgttt actcaaatgg taattaagaa aaacgccaat cttattccag aatattacca
     3121 cggggagttt ctgctctata ccaatgatat agtgacaggc tatgatgaat ttctaagctc
     3181 gcatcaacag tattatgcga ctgaaattca atatcaagtt gaatacgatg aagagacctt
     3241 ccttgagcaa ggtgaaaaaa tagctgggcg ggtatttatt accaccactc gacctaatga
     3301 gccagtcaag agaatcgaag tcattttgat tgcccaatat aaagatgaaa agatttatcg
     3361 attatgggag ttaacttatc cggattggtc caagttacct gccttttcaa atcagctaac
     3421 ctagacgatt ctcttctcct ccaaacaggg gaatatcaga aaagtagttg gtcaagattt
     3481 ccccgcttta catggtgtag cgccacgcag gagcgagcaa ggccttatgg tttaatcaat
     3541 atgagcaaaa aaatgcaaaa cattcctgct tgcgtcagga ttatattatt gatgacaaat
     3601 ttacgactat acttatagta ttaatcaatt gatataaaac atcttgtcta tgaaaggaat
     3661 catattcaac gaatttctca attttgtaga aaaaagtgaa tcctacaccc tggtagatca
     3721 aattattatg gatagtcatt tgaagtccca tggtgcctac acgtctatcg gtacatactc
     3781 tcccaaagaa ttatttcaat tggttaaagc gcttgctatg aaaaatggca aaccaacatc
     3841 agtgatttta caagaatatg gtgagtattt gtttgaggtt tttgcaaaaa aatatcctca
     3901 atttttcagg gaaaaaaagt cggtgtttca atttttggaa gcgcttgaaa cacatattca
     3961 tttcgaagtg aaaaaattgt atgactatac tgaactaccc cattttgaat gccaatatca
     4021 cagtcaaaat caaatggaaa tgatttacac ttcttcgcgt cctttggccg atttt
//
LOCUS       DACTJE010000214         3979 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.216, whole genome shotgun sequence.
ACCESSION   DACTJE010000214 DACTJE010000000
VERSION     DACTJE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.216"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..838)
                     /gene="sidD"
                     /locus_tag="I9760_002803"
     CDS             complement(<1..838)
                     /gene="sidD"
                     /locus_tag="I9760_002803"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector deAMPylase SidD"
                     /protein_id="HAT5044547.1"
                     /translation="MVYYEIIKDIVFTYNLQFTHLIHNDRISEVNLGGVTMRSIITQI
                     CNGVLHGQSYQSGSNDLDKGNSEIFASSLFVHLNEQGKEIIKHKDSDDKIVIGYTKDG
                     MAFQIVVDGFYGCERQAVFSFIDNYVLPLIDNFSLDLTRYPDSKKVTESLIHTIYSLR
                     SKHAPLAEFTMSLCVTYQKDEQLFCAGFGIGDTGIAIKRNEGTIEQLVCHTEVDGFKD
                     AFDNYSSANIDLVIERNSVFNTKVMPGDELVGYTYVPPMLEMTEKEFEVETVDGKKIN
                     KRI"
     gene            908..2851
                     /locus_tag="I9760_002804"
     CDS             908..2851
                     /locus_tag="I9760_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional virulence effector protein DrrA"
                     /protein_id="HAT5044548.1"
                     /translation="MSIMGRIKMSVNEEQFGSLYSDERDKPLLSPTAQKKFEEYQNKL
                     ANLSKIIRENEGNEVSPWQEWENGLRQIYKEMIYDAFDALGVEMPKDMEVHFAGSLAK
                     AQATEYSDLDAFVIVKNDEDIKKVKPVFDALNNLCQRIFTASNQIYPDPIGINPSRLI
                     GTPDDLFGMLKDGMVADVEATAMSILTSKPVLPRYELGEELRDKIKQEPSFSNMVSAK
                     KFYNKAIKDFTAPKEGAEVVSVKTHIMRPIDFMLMGLREEFNLYSEDGAHLSAPGTIR
                     LLREKNLLPEEQIARIESVYNQAMSKRFELHAEHKKEHDEMPYSDAKAMLDEVAKIRE
                     LGVQRVTRIENLENAKKLWDNANSMLEKGNISGYLKAANELHKFMKEKNLKEDDLRPE
                     LSDKTISPKGYAILQSLWGAASDYSRAAATLTESTVEPGLVSAVNKMSAFFMDCKLSP
                     NERATPDPDFKVGKSKILVGIMQFIKDVADPTSKIWMHNTKALMNHKIAAIQKLERSN
                     NVNDETLESVLSSKGENLSEYLSYKYATKDEGREHRYTASTENFKNVKEKYQQMRGDA
                     LKTEILADFKDKLAEATDEQSLKQIVAELKSKDEYRILAKGQGLTTQLLGLKTSSVSS
                     FEKMVEETRESIKSQERQTIKIK"
     gene            2987..>3979
                     /locus_tag="I9760_002805"
     CDS             2987..>3979
                     /locus_tag="I9760_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HAT5044549.1"
                     /translation="MIDNTVKIIKHKVGLLNLAEELGNVSKACKVMGLSRDTFYRYKS
                     AVESGGIDALFDQNRRKPNIKNRVDESVELAVKEYAIAFPAHGQQRTSNELRKQSIFV
                     SPSGVRSIWLRYELANFKDRLKALEAKVASEGIILTEAQVSALEKKKFDDEACGEIET
                     AHPGYLGSQDTFYVGTLKGVGRIYQQTFVDTYSKVAFAKLYTTKIPITSADILNDKVL
                     PFFEQYNLPILRILTDRGTEYCGKVEHHDYQLYLAINNIDHTKTKANSPQTNGICERF
                     HKTILQEFYQVTFRKKIYESIDELQKDLDEWMDYYNNQRTHQGKMCCGRTPMQTL"
BASE COUNT         1387 a          651 c          797 g         1144 t
ORIGIN      
        1 caattctttt atttattttc ttgccatcaa cagtttctac ttcaaactct ttttcagtca
       61 tttctaacat cggtggaaca taagtgtatc ctactagttc atcaccgggc atgactttag
      121 tattaaagac agaatttctt tcaatgacca agtcgatatt ggcggatgaa tagttgtcaa
      181 atgcgtcttt gaatccatca acttccgtat gacaaactaa ttgttcaatt gttccttcgt
      241 ttcgtttgat agctattccg gtgtcaccaa tgccgaatcc agcacaaaac agttgttcat
      301 ccttttgata agttacgcat aaagacatag tgaactctgc caatggagca tgtttgctgc
      361 gcaaagagta aatagtatga atgagtgatt cggtaacttt ttttgaatcc gggtatctgg
      421 tgagatctaa ggagaagtta tcaattaaag ggagtacata gttatcaata aatgaaaaaa
      481 cagcctgacg ttcgcatcca taaaagccat ccaccactat ttgaaaagcc attccatctt
      541 tggtatatcc aatgactatt ttatcgtcag aatccttgtg ctttataatt tctttgccct
      601 gttcattcaa atgtacaaag aggctggatg caaagatttc actattaccc ttatctaaat
      661 cattggaacc agattgatag ctttgtccat gaagaacacc attacagatt tgtgtaataa
      721 tcgaacgcat cgttactcct cctaaattta cttcagatat cctgtcattg tgtattagat
      781 gtgtaaattg taaattatat gtaaagacaa tatccttaat gatctcataa tataccaaat
      841 gatatactta attacaagta gagaggtatt atctatctta tctgaagact tatgaatcct
      901 cgagtacatg agcataatgg ggagaattaa aatgagtgtt aatgaagagc aatttggtag
      961 tttatacagt gatgagcgag acaaaccatt actatcccca accgctcaga aaaaatttga
     1021 agaatatcag aacaaattgg ctaatctaag taaaataatt cgagaaaatg agggcaatga
     1081 agtatctcca tggcaggagt gggagaatgg attacgtcaa atttacaaag aaatgattta
     1141 tgatgccttt gacgcgcttg gtgtcgagat gccgaaagat atggaagtcc attttgcagg
     1201 ttctcttgcc aaggcacaag ccactgagta tagtgatttg gatgcctttg ttattgttaa
     1261 aaatgacgaa gatataaaaa aagtaaaacc ggtctttgac gcattaaata atttatgtca
     1321 gcgtattttt actgcaagca atcagatata tcctgatcct attggaatca atccaagccg
     1381 tcttattggt acgcctgatg atttgtttgg tatgttaaaa gacgggatgg tagctgatgt
     1441 tgaagcaact gcaatgtcaa ttttaacatc aaaacctgtt ctacctcgct atgaattagg
     1501 agaagagtta cgagataaaa tcaaacaaga accttctttc agcaatatgg tttcagccaa
     1561 gaagttttat aataaagcaa ttaaagactt tactgcacct aaagagggtg cagaagtagt
     1621 gagtgtcaaa actcatatta tgcgtcccat cgattttatg ctgatgggat tgcgtgagga
     1681 atttaattta tacagtgagg atggagctca tctttcggca ccaggaacta tacgtttatt
     1741 acgtgaaaaa aatttattgc ccgaagagca aattgctcga atcgagtcag tatacaatca
     1801 agcgatgagc aagagatttg agttgcatgc tgagcacaaa aaagaacatg atgagatgcc
     1861 ttattctgat gctaaagcaa tgcttgatga ggtagcgaaa ataagagagc tgggtgttca
     1921 acgtgtcaca aggattgaaa atctggaaaa tgccaagaag ttgtgggaca acgcgaatag
     1981 catgcttgaa aagggtaaca tcagcggata tcttaaagca gcgaatgagc ttcataaatt
     2041 tatgaaggaa aaaaatctta aggaagatga tttaaggcca gagttgagtg ataaaacaat
     2101 atctccaaaa ggatatgcta ttttacaatc tttatgggga gcagcttcgg attatagtag
     2161 agcggctgca actctcacag aatcaacggt agagcctggt ttagtatcag ctgttaacaa
     2221 aatgtcagca ttctttatgg attgtaaatt aagtccaaat gagcgagcaa cccctgatcc
     2281 agattttaag gttgggaaat ctaaaatttt agtaggtata atgcaattca taaaagatgt
     2341 agcagaccca acttcgaaaa tatggatgca taatacaaaa gctttgatga atcataaaat
     2401 tgctgctatt caaaaacttg agagaagcaa taatgttaat gatgaaacat tagagagtgt
     2461 gctgagctct aaaggtgaaa atttatccga atatctttct tataaatacg caacaaaaga
     2521 tgaaggaaga gagcaccgct atacggcaag cacggaaaac tttaaaaatg ttaaagaaaa
     2581 atatcagcaa atgcgaggtg atgctttaaa aacagaaatc ctggctgatt tcaaggataa
     2641 actggctgaa gctaccgatg aacagagcct taagcaaatt gttgctgaat tgaaaagtaa
     2701 agatgaatat agaattttgg ctaaggggca agggttaaca acccagcttt tagggttaaa
     2761 gacaagttca gtgtcttcat ttgagaaaat ggttgaggaa acaagagaaa gtattaagtc
     2821 tcaagaaaga caaaccatta agataaaata accagcgcat ttcaattatg aagatatgct
     2881 tatatgaatt agtttcgact taatctgaca caactacttg aaaaagtgag agtttccggt
     2941 tttgatagaa gtgcgaactt taaacaaaac caagaggaaa actctcatga tagataatac
     3001 agttaaaatc attaaacata aagttggctt attaaacctt gctgaagaat taggaaatgt
     3061 ttctaaagca tgtaaagtaa tgggcttatc cagagatact ttttatcgct ataagtcggc
     3121 ggttgaatcg ggaggcattg atgctttatt cgatcaaaac cgaagaaaac ctaatatcaa
     3181 aaatagagta gatgaatcag tcgagctagc agtcaaggaa tacgcgatag catttccagc
     3241 acatggtcag cagcgcacga gcaacgagct tcgtaaacaa agtatttttg tttctcctag
     3301 tggtgttcgt agtatttggt tgcggtatga acttgccaat tttaaagacc gtttaaaagc
     3361 attagaggct aaagtagcct ctgaagggat tatattaaca gaggcgcaag ttagcgcttt
     3421 ggagaaaaag aaatttgatg atgaagcatg tggtgaaatt gaaacagccc accctggtta
     3481 cctcggctct caagatactt tttatgtagg tacgcttaaa ggagtcggtc gtatttatca
     3541 gcaaaccttt gttgatactt acagcaaagt agcttttgcc aagctttata cgacaaaaat
     3601 accgattacc tcagcagata tactcaatga caaagtctta ccattctttg agcaatacaa
     3661 cttgcctata ctgcgaattt taactgacag aggcacggaa tattgtggaa aggtagagca
     3721 tcatgattat cagctttatt tggcgattaa taatatcgac catacgaaga caaaagcaaa
     3781 ctcacctcaa acaaatggaa tttgtgagcg tttccataaa acgattttac aagagttcta
     3841 tcaagtaaca tttcgaaaga aaatttacga atcaatcgat gaattacaaa aagatctgga
     3901 cgaatggatg gattactata ataatcaacg aactcatcaa ggtaaaatgt gttgtggtcg
     3961 tactccaatg caaactttg
//
LOCUS       DACTJE010000215         3974 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.217, whole genome shotgun sequence.
ACCESSION   DACTJE010000215 DACTJE010000000
VERSION     DACTJE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.217"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..614
                     /locus_tag="I9760_002806"
     CDS             <1..614
                     /locus_tag="I9760_002806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044550.1"
                     /translation="TLQQSVSLMHEDLKRLPGAVSTETAQTLEPLARLRQDVTQVLEA
                     YDKVTAIQRKTLDELTQQMSASAAQAFEQKAGKLDATISDLSRSLSGLKTSLSSMEQT
                     AQQVATLPGKLASAQQGMTKAADQLTEAANETRPRLWRQALGLILAGAVGAMLVATGQ
                     VALNRLVPPSDVQQTADWANAIWNKATPTERELLKQIANRPAN"
     gene            707..1600
                     /locus_tag="I9760_002807"
     CDS             707..1600
                     /locus_tag="I9760_002807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044551.1"
                     /translation="MTRQQDGRWLWCDRYGNDGGDNIDLVREIEPGTGYAEAVYRLSG
                     APTVRQQPRPSEPKRQPPQLPAQGLAAREHGRDYLKGRGISQDTIEHAEKAGMVRYAD
                     GGVLFVGYDRAGTAQNATRRAIAPADPVQKRDLRGSDKSYPPILPGDPAKVWIVEGGP
                     DALALHDIAKRSGQQPPTVIVSGGANVRSFLERADVQAILKRAERVTVAGENEKNPEA
                     QAKADAGHQKQAQRVAEITGREVRQWTPKPEHGKDLADMNARQVAEIERKRQAEIEAE
                     RARNRELSRKSRGYDGPSFGR"
     gene            1671..2534
                     /locus_tag="I9760_002808"
     CDS             1671..2534
                     /locus_tag="I9760_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT5044552.1"
                     /translation="MAINIMEAFQQEPPQLDFIWPGFLAGTVGALVAPGATGKSFWAL
                     EAAMSIACSVAGGDVVNLKPQHTGRVVYLAGEDPEPALIRRIHVIGQHLNQPAREAIA
                     ENLTVQPIMGAMLNIMDERHRARVIEFCTGARLIVLDTLSRIHSLDENSNGDMARLVA
                     TLEHVAASTGASVLYLHHVSKGSAREGQTDQQQAARGASALIDNARWCGFVAKMTEDE
                     AKRLSDRAYDRQPIGNDRRGFFVRFGVSKQNYDATPLDQWYERRDGGVLVPVELLEVP
                     NGKKGGSREQA"
     gene            2521..3402
                     /locus_tag="I9760_002809"
     CDS             2521..3402
                     /locus_tag="I9760_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C"
                     /protein_id="HAT5044553.1"
                     /translation="MSKHDLTHARHDPAHCLAPGLFRSLKRGERKRGKLDVVYDYGDG
                     KRIEFSGQEPLGADDLRILQGLVAMAGPGGLVLSPEPQTEAGQQLRLFLEPKWEAVQQ
                     DAMVVKGSYRALAREIGYANPDDTKPVRECIERLWKVSIIAQNGRKRQGFRLLSEYAS
                     DEQDGKLYVALNPLIARAVMGGAQHVRIDMDEVRALQTDPARLMYQRLCGWIDPGKSG
                     RVELDTLCGYVWPGQATNPNTLKTRRQAARRALPELAALGWTVSEYAKNKFEIGRPKA
                     THLLGPAPKTQEISHEK"
     gene            complement(3386..3601)
                     /locus_tag="I9760_002810"
     CDS             complement(3386..3601)
                     /locus_tag="I9760_002810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044554.1"
                     /translation="MANRSCFADQYERPVWVWVVRVADHSIKVANVVGNIHALHMQHV
                     GLSILANPSPDNQLFDCRRRFPIISHG"
     gene            3706..>3974
                     /locus_tag="I9760_002811"
     CDS             3706..>3974
                     /locus_tag="I9760_002811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044555.1"
                     /translation="MHKRKFTTLAFYSLALVLLLGLGLGLHQYYIYKVTRETHKTTVD
                     RNLSLMHMRADGYLERLALDGEFIASSPILRMYLENPSAETLAGA"
BASE COUNT          836 a         1235 c         1326 g          577 t
ORIGIN      
        1 ggacgttgca gcagtccgtg agcctgatgc acgaggactt gaagcgccta ccgggcgcag
       61 tctcgaccga gacggcgcag accttggaac cgctggcccg actccggcag gacgtgacgc
      121 aggttctgga agcctacgac aaggtgacgg ccattcagcg caagacgctg gacgagctga
      181 cgcagcagat gagcgcgagc gcggcgcagg ccttcgagca gaaggccggg aagctggacg
      241 cgaccatctc cgacctgtcg cgcagcctgt cagggctgaa aacgagcctc agcagcatgg
      301 agcagaccgc gcagcaggtg gcgaccttgc cgggcaagct ggcgagcgca cagcagggca
      361 tgacgaaagc cgccgaccaa ctgaccgagg cagcgaacga gacgcgcccg cgcctttggc
      421 ggcaggcgct ggggctgatt ctggccgggg ccgtgggcgc gatgctggta gcgactgggc
      481 aagtcgcttt aaacaggcta gtgccgccaa gcgacgtgca gcagacggca gactgggcca
      541 acgcgatttg gaacaaggcc acgcccacgg agcgcgagtt gctgaaacag atcgccaatc
      601 ggcccgcgaa ctagacccga ccgcctacct tgaggccagc ggctacaccg tgaagcgaga
      661 agggcggcac ctgtccgtca gggcgggcgg tgatgaggcg taccgcgtga cccggcagca
      721 ggacgggcgc tggctctggt gcgaccgcta cggcaacgac ggcggggaca atatcgacct
      781 ggtgcgcgag atcgaacccg gcaccggcta cgccgaggcc gtctatcggc tttcaggtgc
      841 gccgacagtc cggcagcaac cgcgcccgag cgagccgaag cgccaaccgc cgcagctacc
      901 ggcgcaaggg ctggcagccc gcgagcatgg ccgcgactac ctcaagggcc ggggcatcag
      961 ccaggacacc atcgagcacg ccgagaaggc gggcatggtg cgctatgcag acggtggcgt
     1021 gctgttcgtc ggctacgacc gtgcaggcac cgcgcagaac gccacacgcc gcgccattgc
     1081 ccccgctgac ccggtgcaga agcgcgacct acgcggcagc gacaagagct atccgccgat
     1141 cctgccgggc gacccggcaa aggtctggat cgtggaaggt ggcccggatg cgctggccct
     1201 gcacgacatc gccaagcgca gcggccagca gccgcccacc gtcatcgtgt caggcggggc
     1261 gaacgtgcgc agcttcttgg agcgggccga cgtgcaagcg atcctgaagc gggccgagcg
     1321 cgtcaccgtg gccggggaaa acgagaagaa ccccgaggcg caggcaaagg ccgacgccgg
     1381 gcaccagaag caggcgcagc gggtggccga aatcaccggg cgcgaggtgc gccaatggac
     1441 gccgaagccc gagcacggca aggacttggc cgacatgaac gcccggcagg tggcagagat
     1501 cgagcgcaag cgacaggccg agatcgaggc cgaaagagca cgaaaccgcg agctttcacg
     1561 caagagccgg gggtatgatg gccccagctt cggcagataa gagcgcctgt cagtcggtgt
     1621 gatgaaccga ccgacaggct aaccatcatc aacctgagta ggaggccgat atggctatca
     1681 acattatgga ggcgttccag caggaaccgc cacaactgga cttcatctgg ccgggctttc
     1741 tggctggcac cgtgggcgca ctggtggccc ccggcgcgac cggcaagagc ttctgggcgc
     1801 tggaagcggc tatgtccatc gcttgcagcg tggcaggcgg tgacgtggtg aacctcaagc
     1861 cgcagcatac cggccgcgtg gtctatctgg ccggtgaaga tcccgaaccc gccctgatcc
     1921 ggcgcattca cgtcatcggc cagcacctga accagccggc ccgcgaagcc atcgccgaga
     1981 atctgaccgt gcagcccatc atgggcgcga tgctcaacat catggacgaa cgccaccggg
     2041 cgcgagtcat tgagttctgc accggggcgc ggctgatcgt gctggacacc ctgagccgga
     2101 ttcacagcct cgacgagaac agcaacggcg acatggcgcg gcttgtcgcc acgctggaac
     2161 acgtcgcggc cagcactggc gcgtctgtcc tgtacctgca ccacgtcagc aagggcagcg
     2221 cccgcgaggg gcaaaccgac cagcagcagg cagcgcgggg cgcatcggcc ctgatcgaca
     2281 acgcccgctg gtgcggcttc gtcgccaaga tgaccgagga cgaggccaag cgcctgagcg
     2341 accgcgccta tgaccgtcag cccatcggca acgaccggcg cggcttcttc gtgcgcttcg
     2401 gcgtgagcaa acagaactac gacgctaccc cgcttgacca gtggtatgag cgccgagacg
     2461 gcggcgtgct ggttccggtg gaactgctgg aagtgccgaa cggcaagaag gggggcagcc
     2521 gtgagcaagc atgacctgac ccacgcccgg cacgacccgg cgcattgtct ggcccccggc
     2581 ctgttccgca gcctcaagcg aggcgagcgc aagcgcggca agctggacgt ggtgtatgac
     2641 tacggcgacg gcaagcgcat cgagttcagc ggccaggagc cgctgggcgc tgatgatctg
     2701 cgcatcctgc aaggacttgt ggcaatggct gggccgggtg gccttgtgct gtcgcctgag
     2761 ccgcagaccg aggccgggca gcaactccgg ctgttccttg agccgaaatg ggaagccgtc
     2821 cagcaggatg cgatggtggt caagggaagc tatcgagcac ttgcccgcga aattggctac
     2881 gccaaccccg acgacactaa gccggttcgg gagtgcatcg agcggctttg gaaggtgtcc
     2941 atcatcgccc agaacggcag gaagcggcag ggcttccgcc tgctgtccga gtacgccagc
     3001 gacgagcagg acggaaagct gtatgtggcc ttgaatcccc tgattgctcg cgccgtcatg
     3061 ggtggcgcgc agcatgtccg catcgacatg gacgaggtgc gggcgctaca gaccgacccg
     3121 gcgcggctga tgtatcagcg cctatgcggc tggatcgacc ccggcaagtc cgggcgggtg
     3181 gaactggaca cgctttgcgg ctacgtctgg ccggggcagg caaccaaccc caatacgctc
     3241 aagacgcgac gccaagcggc tcgcagggcg cttcctgagc ttgccgcatt gggctggaca
     3301 gtcagcgaat acgcaaaaaa caagtttgaa atcggcaggc cgaaagcaac gcatctgctc
     3361 ggcccagcac ccaaaacaca ggaaattagc catgagaaat gattgggaaa cgccgccgac
     3421 agtcgaagag ctgattgtcc gggcttggat tggcgaggat ggacagcccg acgtgctgca
     3481 tgtgcaaggc gtggatgttg ccgacgacat tggcaacctt gatcgagtga tcggcgaccc
     3541 ggacaaccca gacccagaca ggccgttcgt actggtcggc aaagcaggaa cgatttgcca
     3601 ttcagttcgg agaccgtatc tgcgtcaatt gaagtccgtc ccgtttacac aaaaattcgg
     3661 acacgccctg tgtgaggcct caaaatctcc ggaaagacaa cgttcatgca caaacgaaaa
     3721 tttaccactc tcgccttcta ttcgttagcg ttggtgcttt tgctgggact gggtctcggc
     3781 ttgcaccagt actacatcta caaggtcact cgtgagacgc ataaaaccac ggttgatcgc
     3841 aatctgtcgt tgatgcatat gcgggctgac ggctatctgg aacggttggc actggatgga
     3901 gaattcattg cgagctcccc catcctgcgc atgtatcttg aaaacccctc ggcagaaaca
     3961 ctggcgggcg cgca
//
LOCUS       DACTJE010000216         3943 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.218, whole genome shotgun sequence.
ACCESSION   DACTJE010000216 DACTJE010000000
VERSION     DACTJE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.218"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..201)
                     /locus_tag="I9760_002812"
     CDS             complement(<1..201)
                     /locus_tag="I9760_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044556.1"
                     /translation="MNAENNVLNTLNSLSKEINKLYGYVTIAGDNFGEPAINSGPCGP
                     FANAFYKIWNQKFTEKVNIAFIM"
     gene            complement(185..943)
                     /locus_tag="I9760_002813"
     CDS             complement(185..943)
                     /locus_tag="I9760_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5044557.1"
                     /translation="MTKLKNLDTYLSLCTEVYDLSKPNPPEEDYAFYRSYVINVKGPI
                     LEPMCGTGRFLLPLLEEGFEIHGFDASEYMLEALNIKAKAKNLKPTVWRGFVENLTRP
                     EKYNLIFIPSGSFCLLIDPATVRLALQTFYNHLSDEGILLFEGETLKAVPQLDVWRGS
                     VWHKPNGQMIMLSQLATLKDQVCHSIGKYELVHDNSIIHTEVEELRVRIYDPQELTAI
                     LKSCGFKNVRLIKAFDSAATPDAQDETIVYECRK"
     gene            complement(909..1517)
                     /locus_tag="I9760_002814"
     CDS             complement(909..1517)
                     /locus_tag="I9760_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT5044558.1"
                     /translation="MTPHIIGISGITGAGKSTLAKALSLDLKATLISWDDFDDISLEP
                     EDYIEWYHKGCNYSEFQREDLAKVLAELKAKREIMHPVLRTLLNPAEYIIFDAPLGKL
                     HVQTGEYIDTCVHIEVPLDISLCRRILRDFKDKLQTKENLLEEISFYLNHSRPLFFDD
                     ELKQNATIVIDGLLSTEVQIQIVKNFLIGKQDDKIKKPGYLS"
     gene            complement(1514..1981)
                     /locus_tag="I9760_002815"
     CDS             complement(1514..1981)
                     /locus_tag="I9760_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5044559.1"
                     /translation="MITIDLLKNHPQSIARCATIWHEILGKIWVPEIPVEQVIQRFSE
                     HLNADKLPITFIALDTGKPVGMCSLRENDGIRPEISPWLGSLVVDPAYQRQGIGNRLI
                     EATKNKARDLGFNKLHLFAFDPTIPNYYSRLGWAIIGMDKFKGHPVTVMEILL"
     gene            complement(2133..2696)
                     /locus_tag="I9760_002816"
     CDS             complement(2133..2696)
                     /locus_tag="I9760_002816"
                     /inference="COORDINATES: protein motif:HMM:NF024698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAT5044560.1"
                     /translation="MNINFEKANASHIELIFSWLKEPFVQEFWDNTQAHKDDILNFIN
                     GRTEPSSYADGKYVYWIASGNGHPFAMLMTIQETSEDEIDEIKLINLSKTGHSYGIDY
                     MIGDKNYFGIGYGARTLIEFIDFFIKEVDPKADTFLIDPASDNPRAKHVYIKAGFEYV
                     ADFLMSGDVSGAGKLHHLLIKRIGLIK"
     gene            complement(2728..2804)
                     /locus_tag="I9760_002817"
     tRNA            complement(2728..2804)
                     /locus_tag="I9760_002817"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2768..2770),aa:Pro,seq:cgg)
     gene            3107..3733
                     /locus_tag="I9760_002818"
     CDS             3107..3733
                     /locus_tag="I9760_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HAT5044561.1"
                     /translation="MADGDIEIKAGFVDTDLDDRKLTMIDDLNNPLAIVERVYLIWWH
                     WADFHLHVISPHIDTITPAIVIEPELISGSNEHEFVYSIHDSGSKLSTSKSQDMFSAG
                     MSMCKLFYTIEKMVHILVDRLKSGGVSMEEEVQIAFAGHEIAQRKAFESIINLPYNVV
                     VTNFDPGIWGEKYLQNVKRLADKGYGYPPESPRENYKHPVSSATTARK"
     gene            complement(3763..>3943)
                     /locus_tag="I9760_002819"
     CDS             complement(3763..>3943)
                     /locus_tag="I9760_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAT5044562.1"
                     /translation="VGMKAWRFKLTTDSNKTLSRQQIIARFFYFIPATLIAPFCLKGN
                     YVLLHQWTQTRFKKV"
BASE COUNT         1197 a          723 c          731 g         1292 t
ORIGIN      
        1 cataataaat gcaatattta ctttttcagt aaatttttga ttccaaatct tgtaaaaagc
       61 attggcaaat gggccacaag ggcctgagtt aattgcaggt tcgccaaaat tgtccccagc
      121 aatggtgacg taaccataga gtttatttat ttctttagat aagctattta gggtattaag
      181 aacattattt tcggcattca taaacaattg tctcatcttg ggcatctgga gttgcagcac
      241 tatcaaatgc tttaatcaaa cggacatttt taaaaccaca acttttaaga attgctgtta
      301 actcctgcgg gtcgtaaatt cttaccctca attcttcaac ctctgtatga attatactat
      361 tgtcgtgaac gagttcatat ttaccgatag aatgacaaac ctgatccttc agggtcgcta
      421 attgactgag cataatcatc tggccattgg gcttatgcca aactgagcct ctccagacat
      481 cgagttgtgg cactgctttg agtgtttcgc cctcaaacaa taaaataccc tcatcgctta
      541 ggtgattata aaatgtttga agtgctagtc ttactgttgc aggatcgata agcaagcaaa
      601 aggaaccgct tggtataaaa atcaaattgt atttttctgg cctcgttaaa ttttcaacaa
      661 aacctctcca tactgttggt ttgagatttt tagctttggc tttaatattg agggcttcaa
      721 gcatatattc gcttgcatca aagccatgta tttcgaatcc ttcttcaagt aatggaagta
      781 aaaaacgacc ggtaccacac attggctcta aaatagggcc tttcacattt attacataac
      841 tgcgataaaa tgcataatcc tcttcaggag gattgggttt gcttaagtca tagacctcag
      901 tgcacaagct aagataagta tccaggtttt ttaattttgt catcttgttt accaattaaa
      961 aaatttttga ctatttgaat ttgcacttcg gtacttagga gaccgtcaat gactatagtg
     1021 gcattttgtt tcagttcatc atcaaaaaac aaaggtcttg aatggtttaa ataaaaactg
     1081 atttcttcaa gaagattttc tttagtttgt aatttatctt taaaatccct caaaatgcga
     1141 cggcacaatg aaatatcaag tggcacctca atatgaacac aggtatcaat atattcacct
     1201 gtttgtacgt gaagtttccc taagggagca tcaaaaatta tgtattccgc agggttgagc
     1261 aaagtgcgta agactggatg cataatttct ctttttgctt taagctctgc caaaaccttt
     1321 gcaaggtctt ccctttgaaa ttcactgtaa ttacaaccct tatgatacca ctcgatataa
     1381 tcttcgggtt ctaaagaaat gtcatcaaag tcatcccatg aaataagagt tgcttttaaa
     1441 tccagggaca atgcttttgc caaagtggat tttccagcgc ctgttatacc gctaatgcca
     1501 ataatatgag gagtcataat aatatctcca ttacagtgac tggatgtcct ttaaatttat
     1561 ccatgccgat tatggcccaa ccaaggcggg agtaataatt aggaatggtt gggtcaaagg
     1621 caaaaagatg caatttatta aaacctaaat ctctggcttt attttttgtg gcttcaatta
     1681 gcctgtttcc aataccttgt ctctgatagg cagggtcaac cacaagtgag ccaagccatg
     1741 ggcttatttc tggcctgata ccatcatttt cacgcaaaga gcacatccca acgggtttcc
     1801 cagtatcaag tgctataaac gtaatgggta gtttgtcagc atttaggtgc tcactgaacc
     1861 gttgaataac ttgttcaacc gggatctcgg gaacccagat tttcccaagt atttcatgcc
     1921 aaattgtcgc acaccttgct atgctttgtg gatgattttt gagaaggtcg atggtgatca
     1981 tgctttacct gcattgacaa gttcaggtat taaacttttg aggcgtagaa tagtagttgg
     2041 taacatttgt ttgagcacaa ggatcttagc atgaaaagca aaagacttat tgaggtcttt
     2101 aattgctaaa gtaatgtgat ttattccggt aatcatttga ttaaaccaat gcgtttaatc
     2161 aataaatgat gaagtttacc cgcgccagag acgtcacctg acatcaaaaa atcagcaaca
     2221 tactcaaaac ctgcttttat ataaacatgc ttggctctgg gattgtcact tgcgggatca
     2281 attaaaaacg tatcggcttt cggatcgact tcttttataa aaaaatcaat aaattctatc
     2341 aaggttctgg ctccataacc tatgccaaaa tagtttttat caccaatcat gtaatcgata
     2401 ccataagaat gtccagtctt ggaaaggtta atcaatttaa tttcgtcgat ctcatcttcg
     2461 cttgtttcct ggattgtcat cagcattgcg aaaggatgcc cattaccgct tgctatccaa
     2521 taaacatatt taccgtccgc ataggatgat ggttctgtgc ggccattgat aaagtttaag
     2581 atgtcatctt tatgagcttg tgtgttgtcc cagaactcct gaacaaaggg ttctttaagc
     2641 cagctaaaga taagctcgat atgacttgca ttggcttttt caaaatttat gttcattatg
     2701 tggaaaattc taatttttga ttttatatgg tcggagtgac aggaattgaa cctgcgaccc
     2761 ctgcctcccg aaggcagtgc tctaccagtc tgagctacac tccgcgttta tttgaagttt
     2821 atttaattaa tttaatgact taagaaagta tacgaattat tttacttgaa gtgaatttat
     2881 ccgtcgattt attattgatt tgagtactta aatttgcctc aaaataaccc ttaatttatc
     2941 catataaaac gatataaaca caaattatta tgaaaaagcg ggtatctatt ttatgctata
     3001 cattgatttt acggcttttt taccttttct taaggtaggt ttaatgttct attgtcaatt
     3061 ttgggctaat atatcaataa tatccttact acccgggtaa atataaatgg cagacggcga
     3121 tatcgaaatc aaagcaggtt tcgtagatac tgatctagac gatcgcaaac tgacaatgat
     3181 agatgatttg aataatccat tagcgatagt ggagcgagtc tatctgatat ggtggcattg
     3241 ggcagatttt catcttcatg ttatttctcc acatattgat actattactc ctgcgatagt
     3301 catcgaaccc gaactgatat ctggttctaa tgagcatgaa tttgtttatt ccattcatga
     3361 tagtggctct aaattatcta catccaaaag ccaggatatg tttagtgcgg gcatgtctat
     3421 gtgcaaactt ttttatacta ttgaaaaaat ggttcatatt ctggtagatc gcttaaaatc
     3481 tggcggtgtc agtatggagg aagaggttca gatagctttt gctggccatg aaatagccca
     3541 gagaaaggct tttgagtcaa ttattaattt accttataat gttgttgtga ctaattttga
     3601 tcccggaata tggggtgaaa aatatttaca aaatgtgaaa cgcctggcgg ataaagggta
     3661 tggttatcct cctgaatccc caagagaaaa ttataagcat ccggtatctt ctgcaactac
     3721 tgcacgaaaa taaataatta gcgagaatgg ctcaacagtg ttttaaactt ttttaaaacg
     3781 cgtttgggtc cattgatgca aaagcacata attccccttt aagcaaaaag gtgctattaa
     3841 ggtggcagga ataaaataaa aaaatcgggc gataatttgt tgtcttgata gtgttttatt
     3901 agaatcagtt gtgagcttaa atctccaggc tttcatacct aca
//
LOCUS       DACTJE010000217         3942 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.219, whole genome shotgun sequence.
ACCESSION   DACTJE010000217 DACTJE010000000
VERSION     DACTJE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.219"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            404..500
                     /gene="ffs"
                     /locus_tag="I9760_002820"
     ncRNA           404..500
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="I9760_002820"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            526..2196
                     /gene="dnaX"
                     /locus_tag="I9760_002821"
     CDS             526..2196
                     /gene="dnaX"
                     /locus_tag="I9760_002821"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAT5044563.1"
                     /translation="MSYLALARKWRPRTFSQLVGQEHINKALINALNQQRLHHAYLFT
                     GTRGVGKTSVARILAKALNCEKGITSEPCLHCDTCIAIEQGRYIDLIEIDGASKTRVE
                     DTRDLLDNIQYAPTNGRFKVYLIDEVHMLSQHSFNALLKTLEEPPSHVKFILATTDPQ
                     KLPVTVLSRCLQFNLKHLPAELINQQLQLILQEERLDFEIQALDILAQAARGSMRDAL
                     SLLDQAITSCETKLLASDVKMLLGHTQQDYALLLLQALAKQEAPIMLVLSQQIIAEGG
                     NFQYVLDEILSYLHQITVYQCLGDNNPLISPKAEIKELAKQFSAADTQLFYQIGIKGR
                     EEMHLAPTLAIGFNMTMLRMYTFKPASHVTTPPLAFEQNNKTCSLIVDDNTPAQNPIG
                     IEPIPVIKATDNTVAYQQTQSLSTDDKKSANSGESMSCDWNEIIPQLKLSGLALNAIE
                     NAEFLGKKEREITFGVHKGHQSLFTPTTINRIETALAGYYKSPVKIILNTQDAVHASP
                     AQQKEKASQEKLEQAEAALHADPVFQQLQQEFSAELVKNSIVLLKDDL"
     gene            2230..2568
                     /locus_tag="I9760_002822"
     CDS             2230..2568
                     /locus_tag="I9760_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAT5044564.1"
                     /translation="MDINQNLGNLMKEAQKMQQRMQEAQQQLSQLVVSGESGGGMVTI
                     KMNGRHDVTEVKIKPTLMDEDIEMLEDLIAAAVNDAVRKIEKASKEKISQLTAGLNIP
                     TDLMGDKEGE"
     gene            2577..3170
                     /gene="recR"
                     /locus_tag="I9760_002823"
     CDS             2577..3170
                     /gene="recR"
                     /locus_tag="I9760_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAT5044565.1"
                     /translation="MDALSRLVEALRCLPGVGPKSAQRMVFHLLQHQRQRGLHLASCL
                     EQAMKHISHCQQCNNYTEQTLCALCQNPNRDSTLLCVVESPADVSAIEQSNSFQGKYF
                     VLMGKISPLDGLGPDDIGLPKLKELITREKIQEVILALSPSVESQTTIHFIHQLLKDE
                     TVNISQLAHGIPSGGELEFLDGNTISSALKNRAVINV"
     gene            3163..>3942
                     /locus_tag="I9760_002824"
     CDS             3163..>3942
                     /locus_tag="I9760_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HAT5044566.1"
                     /translation="MSKSIFILCRKKILVLLLLSLCFITSTASASFYRSLWPKWLVNT
                     PLSKQSISHQAWQHFLDHHVITNEEDINLVDYTNINEKELASLKEYIKNLSQIDIDNY
                     NRQEQLAYWINLYNALTVLTVANYYPIANIQEINISPGLFSVGPWGANIITIKNTNLS
                     LDDINNRIIRPIWNDPRTHYALNNATIGAPNLSKQAYQGPLLEQQLNDAAFKYINSLR
                     GVHVIEGKLIVSKLYDWYEEDFGGTKKYVIKHLLQFAKEPLR"
BASE COUNT         1301 a          778 c          737 g         1126 t
ORIGIN      
        1 tcaaaaagca gaaagcctca ctttgtttgt acagaactaa gatggaaaaa tgcttttgtt
       61 ttacttaatt gctacttaat ggatattgaa gatagtattt gataaatcct atttacttat
      121 tacttattac ttattactta ttacttatta cttattactt attacttatt acttatataa
      181 gtattgttta taacttccac ttctatttcc tgatattttg tagcttaaaa ggaaatattt
      241 attcatgtgg attggatatg ctctttatta gcccaggtta tttaattact tttttaattt
      301 gtatccttta ggaaagtggg cacactcaaa taataaaata gtcattagct ctagcgcagg
      361 aatatttttt ttgttactct tcgctattca aatatggtga ctctattggt cccctcgcga
      421 cgatagattg tgaaccccgt caggcccgga agggagcagc ggtagcagtt gatgcgggcg
      481 ccggggtgtg gctcttagag tcgccgccca acttgtgaaa cacgtatgag ctatctggca
      541 cttgcccgta aatggcgacc acgcacattt tcccaactgg ttggccaaga gcatattaat
      601 aaagcactga tcaacgcgct gaatcaacaa cgtttgcatc atgcttatct atttactggt
      661 actcgcgggg tcgggaaaac cagtgttgcc cgtattttag ctaaagccct caattgtgaa
      721 aaaggcatca cttcagaacc atgccttcat tgcgatacct gtattgccat tgagcaaggc
      781 cgttatattg atctcattga aatagacgga gcctccaaaa ccagagttga ggacacgcga
      841 gatttacttg acaacataca atatgcaccg acaaatggcc gttttaaagt ttatctaatt
      901 gatgaagtgc atatgctatc ccaacatagc tttaatgctt tactaaaaac attagaagaa
      961 ccgccttcac atgtaaaatt tattctggca acgactgacc cacaaaaatt acctgttact
     1021 gtcttatcgc gttgtttgca atttaatcta aaacacttgc ctgctgagtt gataaatcaa
     1081 caattgcaat taattctcca agaggagcgc ttggattttg aaatacaagc gctagacata
     1141 ttagctcagg ctgcccgcgg aagcatgcgc gatgcattaa gtttattgga tcaggccatt
     1201 accagttgtg aaacgaaatt actggcaagt gatgtaaaaa tgctcttggg acatacccaa
     1261 caagattacg cgttgctgct cttgcaagcc ctggcaaagc aagaagcgcc aatcatgctt
     1321 gtattaagtc agcaaatcat tgccgaagga gggaattttc aatacgttct tgatgaaata
     1381 cttagctatc tgcatcaaat taccgtatat caatgcctgg gcgacaacaa tccattaatt
     1441 agtccaaaag cagaaattaa agaattagca aaacagtttt cggccgcgga cacacaatta
     1501 ttctatcaaa taggtattaa gggacgtgag gagatgcatc tcgcccctac actggccatt
     1561 ggctttaata tgaccatgct gcgcatgtat acctttaaac cagcttctca tgtaacaaca
     1621 cctccattag cattcgagca aaataataaa acatgctcac taattgttga tgataataca
     1681 ccagcgcaaa accctattgg aattgaacca atacccgtta ttaaagcgac ggacaacacc
     1741 gttgcctatc agcagaccca atcattatca acagacgata aaaaatctgc taactctggt
     1801 gagtccatga gctgtgactg gaacgaaata ataccacagt taaaattatc gggtttagca
     1861 cttaatgcca tagaaaatgc agaatttctt ggtaaaaaag agcgagaaat tacattcgga
     1921 gtacataaag gtcaccagtc cttattcacc cccacaacaa ttaaccgaat tgaaaccgca
     1981 ttggctggtt attataaaag cccagtaaaa attattttaa acactcaaga tgccgtgcat
     2041 gcctctccag ctcaacaaaa agagaaagct agccaagaaa agctagaaca agcagaggca
     2101 gcattacacg ctgacccagt atttcaacag ttgcaacaag aattttcagc agaattggtc
     2161 aaaaattcta ttgtcctgtt aaaagatgac ttataatact taaactaacc ataaatagac
     2221 gaggctgtaa tggatattaa tcaaaattta ggcaatttaa tgaaagaagc gcaaaaaatg
     2281 caacaacgta tgcaagaagc gcagcaacaa ttaagccagt tggtcgtaag tggagaatca
     2341 ggtggtggta tggttactat taagatgaat ggacgtcatg atgtcactga agttaaaatc
     2401 aaacccactt taatggatga agatattgaa atgctggaag atctaattgc tgctgcagtc
     2461 aatgatgcag tgcgtaaaat tgaaaaagca tccaaagaaa aaatcagcca actgactgct
     2521 ggacttaata ttccaactga tttaatgggt gataaagaag gagagtaacc ctgactatgg
     2581 atgctctaag ccgattggta gaggcgcttc gctgtcttcc cggtgtggga ccaaaatcag
     2641 cacaaaggat ggtgttccat ttattgcagc accaaaggca gcgaggttta caccttgcct
     2701 cctgtttaga acaggcaatg aagcacatta gtcattgtca gcagtgtaat aattacaccg
     2761 aacaaacact gtgtgctctt tgtcaaaatc ctaatcgaga ttcgacattg ctttgtgtgg
     2821 tagagagtcc agcagatgtt tctgccatag aacaaagcaa ttcctttcaa ggtaagtatt
     2881 ttgtccttat gggaaaaatt tctcctcttg atggcttggg acctgatgac ataggtctac
     2941 cgaaacttaa ggagttaatt actagggaaa aaattcagga agttattctt gcattaagcc
     3001 ccagtgttga aagccagacc acaattcatt ttattcatca gttgctaaag gatgaaaccg
     3061 tcaatatcag tcagttagca catggaatac catcaggtgg tgaattagaa tttttagatg
     3121 ggaatactat aagcagtgcc cttaagaaca gagctgtgat taatgtctaa atccatattt
     3181 attttatgca gaaaaaaaat actggtctta ctcctgttat ccttatgttt cataacaagt
     3241 acagcttctg cttccttcta cagaagttta tggcccaaat ggttggtcaa tacccctctt
     3301 tccaaacaat caatttctca tcaggcatgg caacattttc ttgatcatca tgtaataact
     3361 aatgaagaag acatcaatct ggtagactat accaatatca atgagaaaga gttggcttca
     3421 ctaaaagaat atataaaaaa tttatcgcaa atcgatattg ataattataa ccgtcaagaa
     3481 caattggctt attggatcaa tttgtataat gcccttacag tattaacagt agccaactat
     3541 taccccattg caaacattca ggaaattaac atttctcccg gattatttag cgttggacct
     3601 tggggagcta atattatcac aatcaaaaat actaacttat ccctggatga tatcaataat
     3661 agaattattc gcccaatttg gaatgaccca agaactcatt atgctttaaa taatgccacc
     3721 ataggagctc ctaatctgag caaacaagca taccaagggc cattgctgga gcaacaactt
     3781 aacgacgcgg cattcaaata cattaactct ctcaggggag ttcatgtaat tgaaggtaaa
     3841 ttaattgttt ctaaattata tgactggtat gaagaagatt ttggtggtac caaaaaatat
     3901 gtcatcaaac atttgctaca attcgcgaaa gaacctctgc gt
//
LOCUS       DACTJE010000218         3897 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.220, whole genome shotgun sequence.
ACCESSION   DACTJE010000218 DACTJE010000000
VERSION     DACTJE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.220"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1743
                     /gene="sdeC"
                     /locus_tag="I9760_002825"
     CDS             <1..1743
                     /gene="sdeC"
                     /locus_tag="I9760_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdeC/LaiC"
                     /protein_id="HAT5044567.1"
                     /translation="LPLREGVFDRLSHRLSLETSDNKISPERRDFLNQHVIPVLQECH
                     IALRANDMDMMQKALAKFPTDKQWSAFKSGEAVRAKAQMDVLKQQIEKKIMLQTQIIP
                     ALTECGEALDKQNVTEALQALNKLPAEKEIGKVKTIGQELRGQIVGVKQELTGNLEPL
                     QRATTTPIVQDAEKIKVRYETLLTDVTKRVTDFEKIKPANLDGYNKAIADLNNIQQEL
                     NLLRNEKIRMHTDKDKAVDFSDIEALDKRLQDVQSKLPTQLLEQTSKDVAKLAKMPEK
                     ITFNDIKSMTSKMNNYLETLELIRNDRIKKHAGSTDPLDMSDLDGLKGQLQTYNQSMA
                     DILLRAAKSSLDKIKDPATFEKESPYIKQCFDHLAELEKTLDDSDKGRKQKEDFTAYK
                     SALMDKQEKAYPEMLQLQYKSEALIMQLRDICKIHHDNLAEARRVRLQQLDSQGGGLL
                     GGLWAVTNTIGLTTDNVNIEKMQIRMKEQTLRAFKTELTNDKLNTDQVIAFLAKGSPS
                     ELQEALGISKENAEQLHGLLKQLEIKTASTDKLQEIEKLIDEVSTKIGKEPVKQDHTI
                     TIDEEESDDIRYGF"
     gene            1957..3078
                     /locus_tag="I9760_002826"
     CDS             1957..3078
                     /locus_tag="I9760_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HAT5044568.1"
                     /translation="MPIILDPEVLKVAEYVYQERLSKPYTEVGPEWEYNHKTPYATRA
                     TGTGHNLQRFITIDDQKLHRPIHGLAHTMRTLFYSQLMYEAAKRQPHPHRCADGRTIA
                     DLSAQDLKKLNIAQLFFVAGRESEASYGDAYHRYHLYGAKQFEEYARKHLTHLFSEKE
                     IVLYSRCIEDRIGDRFDETAEGYLIHLSHMIDLMRCKSPVEVFIGHSRGVSGIVPTLI
                     QLFGREDGLDIMHYARSLFAATGEAVPYISSSEWPHLGIESDRVERALKIVGPLEVEG
                     QEADAKKTAQAGFSVDGCYGALVKVDTPDWYHQVKEKEDYDVDEVIALPPQITIREEP
                     PKANESFLLSLLRHVFWCCPSNQKRDDENTDVLEKKAQL"
     gene            3131..>3897
                     /gene="sidJ"
                     /locus_tag="I9760_002827"
     CDS             3131..>3897
                     /gene="sidJ"
                     /locus_tag="I9760_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS meta-effector polyglutamylase SidJ"
                     /protein_id="HAT5044569.1"
                     /translation="MFGFIKKVLDFFGVDQSEDNPSETAVETTDVSTKIKTTDTTQEE
                     SSVKTKTVVPTQPGGSVKPETIAPDQQKKHQIKTETTTSTTKQKGPKVTLMDGHVKQY
                     YFARRGETSTHDTSLPPPVKVLSGRSIPLKEIPFEATRNELVQIYLTSIDKLIKSNKL
                     NSIPSQQIASHYLFLRSLANSETDGIKKNQILSLAKPLGTYLASKEPHVWKMINELIE
                     KSEYPIIHYLKNNRAHSNFMLALIHEYHKEPLTKNQSA"
BASE COUNT         1375 a          758 c          763 g         1001 t
ORIGIN      
        1 ttgcctctta gagaaggggt ttttgataga ctctctcatc gcctttctct ggaaaccagt
       61 gataacaaaa tatctccgga acgcagagat tttcttaacc aacacgtcat ccctgtatta
      121 caggaatgcc atatcgcgct tagagcaaac gatatggaca tgatgcaaaa agccttggca
      181 aaattcccta ctgataaaca atggtctgct tttaaatcag gagaagcggt tagggccaaa
      241 gctcaaatgg atgtattaaa gcaacagatc gaaaagaaaa tcatgctgca aactcaaatt
      301 atcccggctc tcacggaatg tggtgaagca ctggataaac aaaatgtcac agaggcattg
      361 caagcgctca ataaactgcc tgcagaaaaa gaaataggca aggtcaagac aataggacaa
      421 gagttaagag gacaaattgt aggtgttaag caagagctga ccggaaatct tgagcccctg
      481 caacgcgcta ccactactcc aattgtacaa gatgctgaaa aaataaaagt acggtatgaa
      541 acgcttctca cagacgtgac caaacgagtc accgactttg aaaaaatcaa accagccaat
      601 cttgacggtt ataataaggc cattgccgat ttaaataaca tacaacaaga attaaacctt
      661 ttgcgcaatg aaaaaattcg catgcatact gacaaagaca aagcagtgga cttttctgac
      721 atagaagcgt tggataagag attacaagac gttcaatcca aattgccaac tcaactttta
      781 gagcaaacgt ctaaagatgt tgcaaaatta gcaaaaatgc cggaaaagat tacctttaat
      841 gacatcaaat caatgacatc caaaatgaat aattatctgg aaaccttgga attgattcgt
      901 aatgacagga tcaagaagca tgctggatct accgaccctc ttgatatgtc ggatttggat
      961 ggattaaaag gtcaactgca aacttataat caaagcatgg ctgatatcct attgcgcgct
     1021 gccaaaagtt ctctagacaa aattaaagat ccagctactt ttgaaaaaga atccccatac
     1081 atcaaacaat gttttgatca tttagcagaa cttgaaaaaa ctctggatga ttcagacaag
     1141 ggaagaaaac aaaaagaaga ctttactgcc tataaaagcg ctttaatgga taaacaggaa
     1201 aaggcttatc cagaaatgct acagttacaa tacaagagtg aagcattgat tatgcaatta
     1261 cgtgatatct gtaaaattca ccatgacaat ttagctgaag caagaagagt gagacttcaa
     1321 caactcgata gtcagggagg cggactttta ggtggattat gggctgtaac caataccata
     1381 ggtcttacta cagacaatgt gaatattgaa aaaatgcaga ttagaatgaa ggagcaaact
     1441 cttagagcgt ttaagacaga acttactaat gacaagctta atacggatca agtcattgcc
     1501 tttttagcaa aaggaagtcc atctgaattg caagaagctt taggaatatc aaaagagaat
     1561 gcagaacaat tgcatggatt gttaaaacaa ctggagatta aaacagcatc aaccgataaa
     1621 ctccaggaaa ttgaaaagct aattgatgaa gtctcaacta aaatagggaa agaaccagta
     1681 aaacaagatc ataccatcac aatagatgag gaagaaagcg atgacataag gtatggtttc
     1741 taaggagtat tttttaagtg tttgttcaat tgttaaatga agtaatgaat agtacaaagc
     1801 tgtggcagat gagaaggcaa aacatcatat tatttgtttc tcatcaagct gctgcttttt
     1861 attttaagga atactaaatt tacttgttga ataataatta ttcgtgttgt agcattaaag
     1921 aatgctatgt tttaatttaa acaaaaggtg gttattgtgc ccataatttt agatccagaa
     1981 gtattaaaag tagcagagta tgtctatcaa gagagattat ccaaacctta cactgaagta
     2041 ggcccggaat gggaatacaa tcataaaacc ccttatgcca ctcgtgctac aggcacaggg
     2101 cataatttgc aacgatttat cacgatagat gatcagaaac ttcatcgccc cattcatggt
     2161 ttggctcata ccatgcgcac tcttttttac agtcaattaa tgtatgaagc ggccaaaaga
     2221 cagccacacc ctcatcgatg tgctgacgga cgcactattg ctgatctgag tgcgcaagac
     2281 cttaaaaagc ttaacatagc gcaattattt tttgtggctg gacgagaaag cgaggcctct
     2341 tatggtgatg cttatcaccg atatcatctg tatggcgcta aacaattcga agaatacgcc
     2401 cgtaaacatt tgacccatct cttttcagaa aaagaaattg tcctttattc tcgatgcatc
     2461 gaagatcgaa taggcgatcg gtttgatgaa actgctgaag gctatctcat tcacttatcg
     2521 cacatgatcg atttaatgcg ctgtaaaagc ccagtagaag tgtttatagg acattcacgc
     2581 ggtgtttcag gtatcgtacc aaccttgatt caactatttg gtcgtgaaga cggtttagat
     2641 attatgcatt atgcacgcag cctttttgct gccactggcg aggctgttcc ttatatcagt
     2701 tcatcggaat ggccccattt gggcattgag tctgatagag tggaaagagc gcttaagatt
     2761 gtaggaccgc ttgaagtgga aggtcaggaa gccgatgcta aaaaaaccgc acaagctgga
     2821 ttttcggtgg atggctgcta tggcgctttg gtcaaagtag acacacccga ttggtatcat
     2881 caggttaaag aaaaagaaga ctatgatgtc gatgaagtga tcgctttgcc accgcaaatc
     2941 actataagag aagagcctcc caaggcaaat gagtctttcc tgttatcact ccttagacac
     3001 gtattttggt gttgtccttc aaatcaaaaa agagacgatg aaaatacaga tgtcttggaa
     3061 aaaaaggcac aactatgaag aggttatgag gcagcttatt ttataatttt cgaggtcatg
     3121 aggacgcagt atgtttggtt tcataaagaa agtacttgat ttttttggtg ttgatcaatc
     3181 tgaagataat ccatctgaga ctgcggtgga aacaaccgat gtttcaacaa aaatcaaaac
     3241 cactgacacc acccaggaag agagttcagt caaaaccaaa acggttgttc ctacccaacc
     3301 tgggggttct gtcaaacccg agacaatcgc acctgaccaa caaaaaaaac accaaataaa
     3361 aacagaaacg actacaagca caaccaaaca aaaaggcccc aaagtaacac taatggatgg
     3421 tcacgtgaaa caatattatt ttgcccggcg aggtgaaaca agcactcatg acacctctct
     3481 gccaccacct gtgaaggtgt taagtgggcg ctctatccca ttaaaagaaa ttccttttga
     3541 agctaccaga aatgaattag tccagattta tctcacttct attgataaac tcatcaaaag
     3601 caacaaatta aactctattc catcacaaca aatcgcctct cactatctct ttcttagatc
     3661 acttgccaac tcagagactg atggaattaa aaaaaatcaa atactgtcat tagccaagcc
     3721 attgggcact taccttgcct ctaaagagcc ccatgtatgg aaaatgatca atgaactcat
     3781 tgaaaaaagt gaatacccaa ttattcatta tttaaaaaat aaccgtgctc attccaattt
     3841 catgctggca ttaattcatg agtatcataa ggaaccatta accaaaaacc aaagcgc
//
LOCUS       DACTJE010000219         3843 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.221, whole genome shotgun sequence.
ACCESSION   DACTJE010000219 DACTJE010000000
VERSION     DACTJE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.221"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            26..430
                     /gene="mutT"
                     /locus_tag="I9760_002828"
     CDS             26..430
                     /gene="mutT"
                     /locus_tag="I9760_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAT5044570.1"
                     /translation="MKVAVAIIIDEKQRILITQRPFHVAHGGFWEFPGGKLEPHESPE
                     DALVREIREELGIIVNEYRFLGYVDYDYPDKHIQLIIFMVTRFTGNPLCQEGQLNMKW
                     VKKEELNINDFPKANHAVFDLIDIPELQYQAL"
     gene            646..1464
                     /locus_tag="I9760_002829"
     CDS             646..1464
                     /locus_tag="I9760_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAT5044571.1"
                     /translation="MITKQNGIYTLTLSLLLFSLQIKAATDYQTYETVCGEPTDMLSF
                     LERGGIATNPCVVPPRSVLISSGYQYQQLIGEGVQHNFPAAAIQLGLPGYFEVDLLLP
                     NYINQTVDPRIGFSQTQLIVNHVLWFNNKWIITASGTFVFPSGSASFGSPNPGGGVIG
                     ILSYNFNSQLNLTGNLGITSQSEPSYDGGQSYTSVNPDLILSWTKNKISLFAEIYGQS
                     KTAPDEGSGFSTDAGVLYQVKKNIAIDFEVDQRITGLLNGVERYYGGGITIQFN"
     gene            complement(1509..2255)
                     /gene="zapD"
                     /locus_tag="I9760_002830"
     CDS             complement(1509..2255)
                     /gene="zapD"
                     /locus_tag="I9760_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAT5044572.1"
                     /translation="MHDHMITFQLATHFLSRIALRLEYLFKTINEACNESHEVIHRFA
                     LKNIIEIVEIVEKPELKSRFIKELIRIEHVLKKANLLNHSELFDDLETQIHVLNHVPG
                     RFSNKIHDDEFLKTLRQIHHPNTKECEFNSPHLVLWFDSDPLMRQKTISQWVSCLKDL
                     EDTVKIYLSLLRGVTQYTPIRANNGFYQHSLSPKSLNHLILLRIDKKLKMIPKIQLGH
                     HSLTIRLYELVTGQEIRDKTIEMEIAFCQI"
     gene            complement(2493..3098)
                     /locus_tag="I9760_002831"
     CDS             complement(2493..3098)
                     /locus_tag="I9760_002831"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAT5044573.1"
                     /translation="MVYSVGLTGNIASGKSTVAEFFSELGINVIYADKIAKELTSKNT
                     PCYQDIISHFGSSVVLNNGELDRKRIRDIIFSNSNERLWLESLLHPVIRKKIEEQLIV
                     CTSPYCLIEIPLLFNKHHYPYLQKVLLVIAPLESQLDRIVKRDHCTKKQALAILATQP
                     NLEQRLEAADDVLINESGLSELKAKVNKLHQKYLREAKIKQ"
     gene            complement(3070..3543)
                     /locus_tag="I9760_002832"
     CDS             complement(3070..3543)
                     /locus_tag="I9760_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044574.1"
                     /translation="MRNYIFYGLLLLIGLSHQGFTAPMMKKGSYWKCVTYDKANKAWT
                     AQSSYRKVAINVAFATCKKESQLPATCKTSISNCEGFINGVSTRPMWRCTAIDITAQP
                     WESNFYSNRDDAALAAQAYCKENSTLPATCYINMVTCANKNEGAHSDGLFSGTNW"
     gene            complement(3540..>3843)
                     /locus_tag="I9760_002833"
     CDS             complement(3540..>3843)
                     /locus_tag="I9760_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5044575.1"
                     /translation="DDYGTGHASISYLQHFPFNYLKIDKLFVQAIGSNDIIESLNDAI
                     IQMAKGLNLVTIAEGVETKDQANYLANNGVRLQQGWYYSKGLSIAELTALLKGEKI"
BASE COUNT         1198 a          639 c          692 g         1314 t
ORIGIN      
        1 ataaagcctg tcatggaagc ctcgtgtgaa agtagcagtt gctatcatta tcgatgaaaa
       61 acaacgtata ctaattaccc aacgtccttt tcatgtcgca catggaggat tttgggagtt
      121 tcctggcgga aaactggagc ctcatgaatc tccagaggat gctttggtcc gagagattag
      181 ggaggagtta ggtataattg taaatgaata tcgttttctg gggtatgtcg attatgacta
      241 tccagataaa cacatacaat taattatttt tatggtaact cgttttacag gcaacccttt
      301 atgtcaggaa ggacagctca atatgaagtg ggtaaaaaaa gaggaattaa atattaatga
      361 tttccctaaa gcaaatcatg cggttttcga tttaattgat atccctgaac ttcaatatca
      421 agctttataa atgtgcgaat agtctaaagt ttgaagccag gacatcatat ttaattgcta
      481 agcacaaaat aaactccttg tttagatgat gttataacag atcagttgtc gagataaaat
      541 caatcagaat ggtcatcaca tagactagag attgcaaaga tagtaacctt ttgtaaatga
      601 ttagattttg tatttcagtt aattgttaat caattggaca gaaaaatgat tactaagcaa
      661 aatggaattt atacacttac tctttcttta ttactattct cacttcaaat caaagctgct
      721 acagattatc aaacatatga aacagtttgt ggtgaaccaa cagatatgct ttcttttctt
      781 gaaagaggtg gtattgcaac taatccttgt gtagtccctc ctagaagcgt attaattagc
      841 tctggatatc aatatcagca attaattggg gaaggtgtac agcataattt tcctgctgct
      901 gcaatacagt taggtttgcc tggttatttt gaggtagact tgttgttacc caattatatt
      961 aatcagacgg ttgatccacg cataggtttt agtcaaactc agcttattgt caatcatgtt
     1021 ctatggttta ataataagtg gattattact gctagcggaa catttgtatt tccatcaggc
     1081 agcgcttctt ttggtagtcc aaatccagga ggtggtgtaa taggaatttt aagttataac
     1141 tttaattccc aattaaatct tacaggtaat ttgggtatta cttctcaatc ggagcctagt
     1201 tacgatggtg gacaaagtta tacgagtgtt aatccagatc ttattttatc gtggactaaa
     1261 aataaaatat cattatttgc tgagatttat gggcaaagta aaacagcacc tgatgaagga
     1321 agcggtttta gtactgatgc aggagttctt tatcaagtta aaaagaatat tgcaattgat
     1381 tttgaggttg atcaacgtat cactggcctt ttgaatggtg ttgagcgtta ttatggcgga
     1441 ggaattacta tacaatttaa ttaatgcaga ctcactttaa cgcaaagcca gcatagcgca
     1501 cagggcatct atatttggca aaatgcaatt tccatctcta ttgttttatc tctgatttct
     1561 tgaccagtga caagctcata aagcctgata gttaaactat gatgccctaa ctggattttg
     1621 ggtatcattt tcaatttctt atctattctt aacaagatca gatggttaag tgatttaggt
     1681 gaaagactat gctgatagaa tccattattt gctctaatgg gagtatactg agtaacacct
     1741 cgtaacaatg acaaataaat ttttacagta tcttccagat ccttcaggca tgacacccat
     1801 tgactaattg ttttttgcct cattagggga tctgaatcaa accacaatac caaatgaggg
     1861 gaattaaatt cgcattcttt agtattgggg tggtgtattt gtcttaaagt ttttaaaaat
     1921 tcatcatcat ggattttgtt actaaatcga cctggtacat gatttaacac atgaatttgt
     1981 gtttccagat catcaaataa ctcactatga ttcaatagat tggctttttt aagtacatgc
     2041 tcaattctaa ttaactcttt gataaatctg ctttttaatt ccggcttttc aactatttca
     2101 acaatctcaa ttatattctt taatgcaaaa cgatgaataa cctcatgaga ctcattacaa
     2161 gcttcattga tggttttaaa gaggtactcc aggcgcaagg caattcttga taaaaaatga
     2221 gttgctagtt gaaacgttat catatggtca tgcatagatt aaggccatcc ttagctttat
     2281 cctataataa tggcaattta aatttttata gatgcacatt gggtaaaaat gggctcctgg
     2341 gtttatttaa aaacaatatt gttttttata aacaaatcta aaaaatcaag ctattaaggt
     2401 gaattttgtg ttaagactaa cataaaagtt gttgaaaata aaaaaatctt taaaatccat
     2461 atattaactg gtcttttgct agaaatttat tgttactgtt tgatttttgc ttccctaaga
     2521 tatttttgat gaagcttatt caccttagct tttaattcac tcaatccaga ttcattgatc
     2581 agcacatcat ccgctgcttc caagcgttgc tctaaattag gttgtgtggc taaaatagct
     2641 aatgcttgct tttttgtgca atgatctctt ttaacaattc tgtctagctg agattctagg
     2701 ggagcaataa ccaatagaac tttttgaaga taggggtagt gatgtttatt aaacagcaaa
     2761 ggaatttcaa taaggcaata tggcgaagtg cagacgatga gttgctcttc aattttttta
     2821 cggatgactg gatgtaatag actttcaagc cataagcgct catttgaatt ggaaaatata
     2881 atatctctta tacgcttccg atcaagctca ccattattaa gaaccactga agaaccaaaa
     2941 tgtgaaataa tatcctggta gcagggtgta tttttagaag ttaattcttt ggcaatttta
     3001 tctgcataaa taacatttat acccagttcg gaaaaaaatt ctgcgactgt acttttgcca
     3061 ctggcaatat taccagttag tcccactgaa taaaccatct gagtgggctc cttcattttt
     3121 gtttgcgcaa gttaccatat taatataaca agtagcaggt agtgtactgt tttctttaca
     3181 ataggcctgg gcagctaagg ctgcatcatc ccgatttgag taaaaattac tttcccaagg
     3241 ttgggcagta atatcgatgg ctgtacagcg ccacattggt ctggtgctta ctccatttat
     3301 aaatccttca caatttgata tggatgtctt gcatgttgcg ggaagttggc tttctttttt
     3361 gcaagtagca aatgcaacat tgatagccac ctttcggtat gagctttgcg ccgtccatgc
     3421 tttgttggct ttatcataag ttacgcattt ccaataactt ccttttttca tcattggggc
     3481 agtaaagcct tgatgggata aaccaattaa tagtagtaaa ccataaaaaa tataattcct
     3541 catatttttt ctcctttaag caaggctgtt aattcagcaa tggatagccc tttggaataa
     3601 taccagcctt gttgtaaacg aacaccatta tttgccaggt aattagcttg gtcttttgtt
     3661 tccacgcctt ctgctatagt aactaagttt aatccttttg ccatctggat aatggcgtca
     3721 tttaatgatt cgataatatc attagaacca attgcttgaa caaataattt atcaattttt
     3781 aaataattga atggaaaatg ttgcaaataa ctaatgctgg catggccagt cccataatca
     3841 tca
//
LOCUS       DACTJE010000220         3689 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.222, whole genome shotgun sequence.
ACCESSION   DACTJE010000220 DACTJE010000000
VERSION     DACTJE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.222"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(131..838)
                     /locus_tag="I9760_002834"
     CDS             complement(131..838)
                     /locus_tag="I9760_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT5044576.1"
                     /translation="MIFHQLFDQDSCTYTYLIGSADSKHGVIIDPVKSHVSTYINLIN
                     ALGLNLVASIDTHLHADHITGSGQLTTLTDCQSMMGIETEAKFVQVKFCDDEILNFGN
                     IKIRAIHTPGHTPDSYCFILDDKVFTGDTLLINATGRTDFQNGSASEQYESLFNKLLK
                     LPGFMKVYPGHDYNQKHFSTIEQEKKNNPRLQVKSRQEYVEMMGNLNLSFPKNMHFAV
                     PANLNNGLTEQEVSALV"
     gene            1330..2298
                     /locus_tag="I9760_002835"
     CDS             1330..2298
                     /locus_tag="I9760_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAT5044577.1"
                     /translation="MTKILIIGAGQLGSRHLQSLNLLTKKLDITVVDPNPGSLEIAKS
                     RFDSTIAEIKHNISYEQEIPTAQTTDIAIIASTANQRRSIIDDLLNKTQVNHLVLEKL
                     LFTQEQDYHDVADKLASLPTRTWVNCTMRMMPYYQHLNTVFYNESIQYHVSGSQYGLV
                     TNAIHYLDHAAYVTGSNNFALNTQYLDKQIIESKRAGYYELTGTLIAHFDNGSLAFLH
                     CNKSGMSPITIEISSENKRIISKEWEQKAWQTSLATDWKWEEVNAPIPYQSTLTAELV
                     TSLLDTNSCNLTDYKTSMQIHLQLLNPLRAFLLENQLDSQMDYPFT"
     gene            2322..3266
                     /locus_tag="I9760_002836"
     CDS             2322..3266
                     /locus_tag="I9760_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAT5044578.1"
                     /translation="MHVAILGAGPMACEYSKVLHSLQIPFTVIGRSQTSIINFQNTTG
                     FTAIAGGYENFFANHQHSFSHAIIAVGEKQLGAATRAAIKAGISSILVEKPGGIDSED
                     IRLVADTAKSNQAHVYVGYNRRFYASVEKAKSIIEEDGGLLSFNFEFTEWGHVIADLK
                     KEEGVKENWFLANSSHVIDLAFYIGGTPKVLNAYTSGNLTWHPNAAIYAGSGITENGA
                     LFSYQANWQAPGRWALEFLTRKHRLYFKPIEKLQIQNLGSVALEEVALDDSLDYDFKP
                     GLFKQTQAFLSNPESLPSIEEQVNMLSWYEQINKPSTL"
     gene            3263..>3689
                     /locus_tag="I9760_002837"
     CDS             3263..>3689
                     /locus_tag="I9760_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044579.1"
                     /translation="MNKFSIISIDHISQLIPGLAALRYSNIKNNTLQKPLLVLSKPHL
                     AINDIKLTHIKALISSYPHSVFICGYSLFKYPKLMTYLPVKLRAKFLTQKLKKYQIDE
                     IFFSHDLSSDFWNQVLMHAFPKAKRICYGDALGLVYSQSY"
BASE COUNT         1286 a          687 c          602 g         1114 t
ORIGIN      
        1 taaaattctg ttcctaaaac tatctgttat tcaaattaca aaggtacctt gagcttaggt
       61 tgcaatttat ctttataaat ccgagacttg gagaatgaat aaaatataac aaaataaatg
      121 ataataatct ttaaaccaga gcgctaactt cctgctcagt taatccatta ttcaagttgg
      181 cgggaactgc aaaatgcatg tttttaggaa aagacaaatt cagattaccc atcatttcaa
      241 catactcttg tctggatttt acctgtaatc tgggattatt tttcttttcc tgttcaattg
      301 tactgaaatg tttctggtta taatcatgcc caggataaac tttcataaaa ccgggtaatt
      361 ttaaaagctt attaaacaga ctctcgtatt gttcgctcgc gctaccattt tgaaaatcag
      421 tacgcccggt agcgttaatt aataaagtat cacccgtgaa tactttgtcg tccaatatga
      481 aacaatagga gtctggagta tggcctggag tatggattgc tcttattttg atgtttccaa
      541 aatttagaat ctcatcatca caaaatttaa cctggacaaa cttagcctca gtctcaatcc
      601 ccatcataga ctgacaatca gttaaagtag ttaattgacc agatccggta atgtgatccg
      661 catgcagatg agtatcaata gaggcaacca aatttaaccc taacgcgtta atcaagttaa
      721 tatacgttga aacatgagat ttcaccggat caatgattac tccatgcttg ctatcagcag
      781 aacctatcaa gtaggtataa gtacaagagt cttgatcaaa caactggtga aaaatcatta
      841 aatctccaca aaaccatcaa gaaaacaaag caatatatta atatagcaat cagcacgatt
      901 agaatagaat gcagtttact ttaagcaaga aattctgcaa acttaaatct aataggtcat
      961 ttcaatttta tcagaacata tgtttaccac acagtcagca atatttgagc aagaccttta
     1021 tagatatata agctcccatg cctcaataaa ttttcaccga ttaattatta tcatacatac
     1081 cattgtatag atcccgaatt aatctaacag tggatcgtgc atgaatttta taattaatta
     1141 ccccactttt aacccatgga tttacccgct accaattatc attattaaaa attgtatacg
     1201 tccagaaacg cgtaaataat tttcggtaat atatgctgca caacaggata aataaagatt
     1261 tttcaaagat ggatttaaat tgattgctca gataaaatat ccgccaaact aactttaatc
     1321 atttaaatca tgacgaaaat attgatcata ggggcggggc aattaggaag cagacatttg
     1381 caatcattaa acttactaac aaagaaactt gacatcactg tagtagatcc taacccgggc
     1441 tcgctagaaa tagcaaaatc tcgtttcgat tcaacgattg cagaaataaa acataacata
     1501 agttacgagc aggaaatacc aaccgctcaa accacagata tagcaataat tgccagcacc
     1561 gcaaatcagc gtagaagcat aatagatgac ctgttaaata aaactcaagt gaatcacctg
     1621 gttcttgaaa aattattgtt cactcaagaa caagattatc acgatgtagc agataaacta
     1681 gcctctctac caacccgcac atgggtgaat tgtactatga ggatgatgcc ttactatcaa
     1741 catctcaata cagtgtttta taatgaatca attcagtatc atgttagtgg tagtcaatat
     1801 ggcttggtaa ccaatgcaat ccactattta gatcatgctg cctatgttac aggttcaaat
     1861 aattttgcac taaataccca gtatcttgat aaacaaatta tagaaagcaa gagagctgga
     1921 tattatgaac taactggaac actgattgct cattttgata atggcagcct tgccttcttg
     1981 cattgcaaca aaagtgggat gtctcctatc actattgaaa tttcaagtga aaacaaacgt
     2041 attatttcca aagaatggga gcaaaaagct tggcaaacca gtttagcaac tgactggaaa
     2101 tgggaagagg taaatgctcc tattccttat caaagtactt taaccgcaga attggttaca
     2161 tctttactag ataccaatag ctgcaaccta actgattaca aaacttcaat gcaaattcac
     2221 ttacaattgc tcaatccctt aagagcattt ttgttagaaa accaattaga tagtcaaatg
     2281 gattatccat ttacctaaaa ttattacaag gacaaatagc tatgcatgtt gcgatattag
     2341 gcgcaggccc tatggcctgt gaatacagta aagtactaca ctctctgcaa attcccttta
     2401 ctgtaatagg cagaagtcaa acttctatta ttaattttca aaatactact ggttttacag
     2461 cgatagctgg gggatatgaa aactttttcg ctaatcacca gcactcattt tcgcatgcta
     2521 ttatagcagt tggagaaaaa caattaggag cagcaaccag ggcagccatt aaagcaggca
     2581 tttcatctat attagttgaa aaaccaggcg gtattgattc tgaggatatt cgacttgttg
     2641 ctgacaccgc taaatcaaat caagcacatg tctatgtggg ttataataga agattttatg
     2701 cttctgttga aaaagccaaa tctattatag aggaggatgg tgggttatta tctttcaatt
     2761 ttgagtttac tgaatggggg cacgtcatag ctgatctaaa aaaagaagaa ggtgtcaaag
     2821 aaaattggtt tttagcaaat tcatcccatg ttattgatct ggctttttat attggtggaa
     2881 cacctaaagt attgaacgcc tacactagcg gtaacctcac ttggcaccct aatgctgcaa
     2941 tttatgctgg ctccggaatt actgaaaatg gagccctatt ctcttaccag gcaaactggc
     3001 aagctccagg gagatgggct ttagagtttt taaccagaaa acatcggctt tattttaagc
     3061 ctatagaaaa gcttcaaatt caaaatttag gtagcgtagc tttagaagaa gtagctcttg
     3121 atgattcatt agactatgat tttaaacccg gtttgtttaa acaaacacaa gcattcttaa
     3181 gtaatcctga atcacttcct agtattgaag agcaggtaaa tatgctctca tggtatgaac
     3241 aaataaataa accaagcacc ttatgaataa attttctata atcagcatag accatattag
     3301 ccaactaatc cctggtctag ctgctttaag atatagcaac ataaaaaaca atactttaca
     3361 aaaaccattg cttgttctaa gcaagcctca tcttgcaata aatgacatca aattaactca
     3421 tattaaagca cttattagca gctatcctca ttcagttttt atatgcggct attccctgtt
     3481 taaatatcct aaattgatga cttatttacc agtgaagtta agagcaaaat ttttaacaca
     3541 aaaactaaaa aaataccaaa ttgatgaaat ctttttttca catgatctga gtagcgattt
     3601 ttggaatcaa gtacttatgc atgccttccc caaagcaaag cgcatttgtt atggtgatgc
     3661 attaggttta gtctacagtc aaagctatt
//
LOCUS       DACTJE010000221         3617 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.223, whole genome shotgun sequence.
ACCESSION   DACTJE010000221 DACTJE010000000
VERSION     DACTJE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.223"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            16..906
                     /locus_tag="I9760_002838"
     CDS             16..906
                     /locus_tag="I9760_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019156728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAT5044580.1"
                     /translation="MDAINDKNPIVALESTIISHGMPYPDNLTTAIEVENIIRRQGAI
                     PATIAMHQGKIRVGLTQEVMEHLALQKEVIKASRRDISFVLSRKVTASTTVAATMFCA
                     HMAKLPLFVTGGIGGVHQDVTMSFDISADLIELSNTPVTVVCSGAKSILDLPKTLEVL
                     ETFGVPVIGYATDEFPAFYSRSSGIPVPQRLNSAEEVANLMSIQQKLNMKNGIVVANP
                     IPVSAELSDEEISPYIKQAHDEAKHMSGKSLTPFLLKRIAELTAGKSLEANIELIKNN
                     AFLGAEIAIAYQKKLFSKKT"
     gene            957..1032
                     /locus_tag="I9760_002839"
     tRNA            957..1032
                     /locus_tag="I9760_002839"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:990..992,aa:Phe,seq:gaa)
     gene            complement(1075..1164)
                     /locus_tag="I9760_002840"
                     /pseudo
     CDS             complement(1075..1164)
                     /locus_tag="I9760_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1211..2362
                     /locus_tag="I9760_002841"
     CDS             1211..2362
                     /locus_tag="I9760_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HAT5044581.1"
                     /translation="MYPFILKPKSLRFFNFVFIALALSFCLLANARAAGIERYDFGDM
                     ASKVTKAWDQDVALYVAKRFAVENSFDEKEITVKKLNAGATNHFIYLMFINNELKFVI
                     KGLGTEKEAESLFALQNYSKIPDIQNNPQAATVTLSKIINSYRVKNDSRTYFFTILEA
                     AQGKELFKIMTYELLQHKDINTLKGVFYRIGQQMAELHKTIIGSDQLITPRDLVTVIH
                     DDFHPENTFYNLETNIFSLIDNESMGDSITEPLPIIRELYGFYEVPIIRWPSEKITVD
                     LLKDADPHDIASIYYDFVLGMASVYQDPQDAKRELIEAIIDLNNMAIAFLKDRYSEPW
                     MLNPSDKHEAHIVWGKLAKVDTNPQLAELYITKLTIINNELQSLSGFKR"
     gene            complement(3154..>3617)
                     /locus_tag="I9760_002842"
     CDS             complement(3154..>3617)
                     /locus_tag="I9760_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044582.1"
                     /translation="HGDSILINGQGDIDKQLIAGRDAEELVDILENDLELKEISLKNL
                     DIDSCMMGRVESYRHELKRHLKNFQTITTYTDLCTASQSGGVPYRMWIEQRADRDVFY
                     TESDLNKKGTRIIEYTDTYKNSLKEIWKTNPYNLEEIDLSEYIDILVIASC"
BASE COUNT         1173 a          698 c          629 g         1117 t
ORIGIN      
        1 tttaacgaag aagtactgga tgctatcaat gataaaaacc caattgttgc tcttgagtcg
       61 accattatct ctcatggaat gccctaccct gataatttaa ctacggcaat agaggttgaa
      121 aatattattc gtagacaagg cgccattcca gctactatag cgatgcacca agggaaaatc
      181 cgtgttggtt taactcaaga agttatggaa catctcgcgc ttcaaaaaga agtaataaaa
      241 gcctctcgca gagacatttc ttttgtatta tctcgtaaag tgactgccag cacgacggta
      301 gcagcaacca tgttttgtgc ccatatggct aagctccccc tatttgtcac cggaggtata
      361 ggtggtgttc atcaagacgt gacgatgagt tttgatattt cagctgatct tatagagcta
      421 tccaatacgc cagttaccgt agtctgttct ggcgcaaaat cgattctcga cttaccaaaa
      481 acattggaag ttcttgaaac gtttggggtg ccggtcatag gttatgccac agacgaattc
      541 cctgcttttt atagcagatc gagtggaata ccagttccac aacgcttaaa ctcggccgag
      601 gaagtagcaa atctcatgtc aattcaacag aaattgaata tgaaaaatgg aatagtcgtc
      661 gcaaacccca ttccagtatc agccgaacta tccgatgagg aaatcagtcc atacataaaa
      721 caggctcatg atgaagccaa acacatgagc ggaaaatcat taaccccctt cctgttaaaa
      781 cgtattgctg aattaactgc gggcaagagc ctggaagcta atattgagct tatcaaaaac
      841 aacgcttttc taggagcaga aattgccata gcctaccaaa aaaaattatt ttcaaaaaaa
      901 acttaatcaa cacttgactc aaagtcgatc tcggagttta atagcaagcc ttgagtggcc
      961 agatagctca gtcggtagag cagaggactg aaaatcctcg tgtcggcagt tcgattctgc
     1021 ctctggccac cattagaatt tccgggtctt tcccaattac ggcgcactcc ccatttaaca
     1081 acacaacacc ctaacacgag ttctataatc atcaaagtta cgaaagccat aagctagtct
     1141 ttgagtcaat ttgatttgca aatgcaaaca acatacaata tgatcaattc taattcatag
     1201 accaattaaa ttgtacccat tcatccttaa acccaaatcc ttgcgatttt ttaactttgt
     1261 ttttattgct ttagcccttt ctttctgttt attagctaat gcccgagcag caggaattga
     1321 acgctatgac ttcggagata tggcctcaaa ggtgactaaa gcttgggatc aagatgttgc
     1381 gctttatgtt gccaagcgtt ttgcagtaga aaacagtttt gatgaaaagg agataactgt
     1441 caaaaaatta aatgcagggg cgaccaacca ttttatatat ttgatgttta tcaataacga
     1501 gttgaagttt gttatcaaag gtctgggaac agaaaaagag gcagagtcat tgtttgcttt
     1561 acaaaattac tcaaaaattc ctgatattca aaataatcca caagccgcga ctgttacatt
     1621 aagtaaaatc attaattcct atagagtaaa gaatgattcc aggacttatt tcttcacaat
     1681 tttagaagct gctcaaggaa aagagttatt taagataatg acttatgaat tgctacagca
     1741 taaagatatt aacactttaa aaggtgtttt ttatcgaata gggcaacaga tggctgaatt
     1801 acataaaaca attatcggga gtgatcaact tatcactcca agagatttag tgaccgttat
     1861 tcatgatgat tttcaccctg aaaatacgtt ttataatctt gaaacaaata tcttctcttt
     1921 aattgataat gaaagcatgg gtgatagtat tacagagcct ttgcccatta tcagagaatt
     1981 atatgggttt tatgaagttc ctatcattcg ctggccctca gaaaaaataa ctgtagatct
     2041 cttaaaggat gctgatcctc atgatatagc cagtatctat tacgactttg ttctcggtat
     2101 ggcctcggtg tatcaagacc cccaagatgc aaaacgagag ttaattgaag ctattattga
     2161 tttaaataat atggctattg cctttctgaa ggatagatat tctgagccat ggatgcttaa
     2221 cccaagcgat aaacatgaag cacatattgt gtggggcaag ttagcaaaag tcgacactaa
     2281 tccgcagcta gcagaactct atatcactaa attaaccatt attaataatg aattgcaatc
     2341 actttcaggc tttaaacgct agcagagggt gagtactcag gttattccgc accctcggat
     2401 tttaattgct gctgcataaa cctctataca tcatctattt atgtatttat ttaaagtgta
     2461 ttgctgcttt atatatcctt tctcattgat gaaagacacc ttggtaaagg atgaatcatc
     2521 tgactaactt ctaaattcca caagaaattt gacaacctat aaacagactt atccacagaa
     2581 aatgtggata acttaaataa aattacaggt attaaaaaat gatatcaacc aagcccaata
     2641 aggattgtta tagtttactg attaatcggt tgtgatttgg taaaataaga tatccacaga
     2701 caacggataa gggaaaatat tcttgaccta cctatcttat acaaataaaa aataaaaaaa
     2761 cagtcttgac tcaaactatt tctactattt attttgaccc gctgcttgat taaacttttt
     2821 gcttaaacaa taactctcct gccttagtga ggtttttaaa tttggaacta tagccatctt
     2881 gtctggaaca actagaatag cgaacaagcc tatgcctgat aaatagacaa actaatatta
     2941 tgtggagatg gtttgttcag gtgttttaaa atcggcaacg tatccattct tctttggatt
     3001 tcaaatctct aataattttg gcatggttgc ttaatgttgg tgtccggctt ttcgaggaag
     3061 gatcagtctg agatgttatg acttaacgca tcacacagtt taaagattga aaaatttgcg
     3121 agttacccaa ctcaattcaa gctattgtcg aaattagcat gatgcaatta ctaaaatatc
     3181 aatatactca gacagatcaa tttcttccag attatacggg tttgttttcc aaatttcttt
     3241 taatgaattt ttataggtat ccgtatattc aataatgcgg gttccctttt tgtttagatc
     3301 actttcagta taaaaaacat ctctatccgc tctttgttct atccacattc tgtaaggcac
     3361 tccaccactt tgtgatgctg tgcataaatc agtatatgtt gtaatagttt gaaaattctt
     3421 taaatggcgt tttaattcat ggcgataact ttcaactctt cccatcatgc aagaatctat
     3481 atctaaattt tttagtgata tttcttttaa ctccaaatca ttttcaagaa tatctaccaa
     3541 ctcctcagca tcgcgccccg caattaattg cttatcaata tcaccctgcc cgttaataag
     3601 tatggaatcg ccatgac
//
LOCUS       DACTJE010000222         3612 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.224, whole genome shotgun sequence.
ACCESSION   DACTJE010000222 DACTJE010000000
VERSION     DACTJE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.224"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(229..1773)
                     /gene="ankG"
                     /locus_tag="I9760_002843"
     CDS             complement(229..1773)
                     /gene="ankG"
                     /locus_tag="I9760_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkG/AnkZ/LegA7"
                     /protein_id="HAT5044583.1"
                     /translation="MNSLWIASGSRDMIYVLICIMLVCGLVVYQLMPKLLSYSTSLIS
                     KKQDTSELTHDGITNVLTQLGHPKFEGVCYGFTLNWALAVAQGKESFFYRQLHHLRTH
                     QFGLPETLQQIKEKKERNQSLSKDEKIIETLPQLGKKICIAQDPLQYKEKYKKLVWQP
                     DINSILKAINADSSVAKHIFYKTHSFLNQDEATEYLELLKRTGIREDVAVIISTADHA
                     MGFKLAGNVWRFININDLYQQDKNKPYFEFSSRNLVKELYRVCAENLQGSRLTVNTDF
                     VSVNPEEKLSRALQNLFPVFPVRTKTSYPERLAFFSMAATQGDMNSVKKCIHSGWSIF
                     SRQRLSDDSPILTAIYLGRRDVVRAMLSTSRNRVNQKRKSDSSTLLHIACRYGGSGIV
                     EDLLNIRGIKIDPRDSKGRTPLMYACKKSVVTEDRKLFNLLFAKGASLSIKDNDGLTA
                     LDHALKNEHTLAIQMIEERLEKEACAQENSTSRRFKFSETKGTLFQRGVKTISYQSQQ
                     PRFGMK"
     gene            complement(1953..>3612)
                     /gene="ankY"
                     /locus_tag="I9760_002844"
     CDS             complement(1953..>3612)
                     /gene="ankY"
                     /locus_tag="I9760_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkY/LegA9"
                     /protein_id="HAT5044584.1"
                     /translation="ILVYANCVNSTAKGDFALAGQIAKDLVREKTSDDIDVVLTSTLD
                     GIARFESLYGKAVDGRVIIEGTSVGLCALELFESVDNEVVAFIEANRCKYAPSDILKR
                     VLSPDSKFLFIGAANQKAIATGDKFTKSWLYISHKGDQPNVYEHFDEDDSLIQSVGLG
                     DDRLGLPSLPKVDELPEMNSTQSQKIPSGEYGFMYLAAVHGIDDVYLMNQYMSLTEMG
                     QYVLVGEYAKQSKEVQMVLHSLQYKGSSVSLTMPQIFFHDSVDNCLMRNMVSKSSGPL
                     VVSTGVMSSIEAMQDGKLTYYQTMSNNTQFVASYLIAVKSICSSDTTLFGSMPKLIID
                     LSNLLFAEKPLKESQMKEIKSLLGMSSVTSRLGEMNKRIIAKANGTVAGELLSFIGKP
                     KTTQSHKQCISVCMSLRKSGETTSPIFDQALRRAAAWGRLFELKVLIASMSTSDLNKK
                     DKTGNQYTALHWAVMQGHLDCARLLVKSGASVDIQDKSGKSPLHYAIKRGDKETIKLL
                     VQAGASLEIMDDSGAKPCDGSESWVPEYIKSCYEKSGHKSSSLF"
BASE COUNT         1130 a          750 c          615 g         1117 t
ORIGIN      
        1 tactgaccta caaaattgac ccaacaggca ttgagaattt tcatttcagt acaggtccga
       61 tgcgaaaaca tcatatgatg ttatgtcatt aaaaaccagt attctgtata taaaattaat
      121 aacaagaatc tttctaatgc acattaagtg gtttgtttac taatagggag caaaacccca
      181 aaatcgagac aaaaagcttg aagtgctaaa aagacaaatt ttattctcct atttcatacc
      241 aaaacgaggt tgctgtgatt gataagaaat agttttcact cctctctgga acaaagtacc
      301 ttttgtctca gaaaatttaa atcgacgaga ggttgagttc tcctgcgcac aagcttcttt
      361 ttccaatctc tcctcgatca tttggattgc aagggtatgc tcatttttaa gagcatgatc
      421 aagtgcggtc aaaccgtcat tatctttaat agaaagagaa gcgccttttg caaacaacaa
      481 attaaataat tttctatcct ccgtcactac agatttttta caggcgtaca tgagcggcgt
      541 tctccccttg ctgtctcggg gatcgatttt aattccacgt atgttcaaca gatcttcgac
      601 tataccacta ccaccatacc gacaggcaat atggagcaaa gtagaagagt cgcttttacg
      661 cttttggtta actctatttc gagaagtgct cagcatggca cgtactacgt ctcttcttcc
      721 taaatagatg gctgtaagga taggtgaatc atcactaagt cgttgtcgag aaaaaattga
      781 ccatccagaa tgaatacact tttttacaga attcatatca ccctgagttg ctgccatgga
      841 aaaaaaagcc aatcgttcag gataagaagt tttggttctg actggaaaca cagggaataa
      901 attctgcagt gctcttgaca gtttttcttc aggattaact gaaacaaaat cggtattcac
      961 ggtcaatcga cttccctgaa ggttttcggc acaaacacga tacaactctt taaccaggtt
     1021 tcgggacgag aactcaaagt aaggtttatt tttgtcttgt tggtataagt cattgatgtt
     1081 aataaaccgc cagacattac cagccagttt aaatcccatg gcgtgatccg cggtactgat
     1141 aattactgca acatcctctc tgatgcctgt tctttttaat aattcaagat attctgttgc
     1201 ttcatcctga tttaaaaaac tatgcgtctt ataaaaaatg tgctttgcaa ccgaagagtc
     1261 ggcatttatg gctttcaaaa tggaattaat atcaggctgc cataccagct ttttgtattt
     1321 ctctttatat tgtaagggat cttgagcaat acaaattttc tttcctaact gaggcaaagt
     1381 ttcaatgatc ttctcatctt tgcttaatga ttggttcctt tctttctttt ctttaatttg
     1441 ttgtaatgtt tcaggcaaac caaactggtg ggttcgtaaa tggtgcaatt ggcggtaaaa
     1501 aaaagattcc ttaccctgag ccaccgccaa tgcccaattg agagtgaagc cataacagac
     1561 tccttcaaac ttgggatgac ctaattgtgt taagacattc gtaataccat catgtgttaa
     1621 ttcactggtg tcctgttttt tggagatcaa agaagtagaa tacgacagga gcttgggcat
     1681 gagttgatac acgaccaaac cgcaaactag cataatacaa attaaaacat aaatcatatc
     1741 ccttgaacct gatgctatcc ataatgaatt cagtataaaa ttaacccata aaataagtcg
     1801 agggggttcg aggttaaatt ttcttcaaaa aacagggtaa taccagaaaa gcaaataaaa
     1861 tcatccacgc tttataaata ataaaaaatt acccggatca tggatttcat ctccgggtaa
     1921 ttcagggcta taaatctgcc attaagatta atttaaaata agctactaga tttgtggcca
     1981 cttttctcat aacaggattt aatatactca ggaacccaag actctgatcc atcacaaggt
     2041 tttgctcctg aatcatccat aatttccaaa gaagctcctg cttgaaccaa taatttaatg
     2101 gtttctttat ctcctctttt aatcgcatag tgtaaaggcg atttaccaga cttgtcttga
     2161 atatcgacag aggcgccaga tttaactaat aatcttgcac aatctaaatg cccctgcatc
     2221 acagcccaat gaagtgctgt gtattgatta cctgttttat cttttttatt taaatcagaa
     2281 gttgacattg atgcaatcaa caccttcaat tcaaacaagc gaccccaagc tgctgctctg
     2341 cgtaatgctt gatcaaaaat aggcgacgta gtttcaccac tcttgcgaag agacatgcaa
     2401 acgctgatgc attgtttatg tgattgcgtc gttttaggtt ttccaataaa gcttaaaagc
     2461 tcaccagcaa cagtcccgtt tgcttttgca atgattcttt tattcatttc tcccaagcga
     2521 ctagtaactg acgacatacc taagagagat ttaatttctt tcatttgact ttctttcaac
     2581 ggtttttctg cgaaaagcaa attagacaaa tcgataatta atttcggcat agacccaaat
     2641 aacgttgtat cactggagca aatggatttc acagcaatca aataagaagc cacgaattgc
     2701 gtgttattag acattgtctg ataatacgtt aatttaccat cttgcatcgc ctcaatagaa
     2761 ctcataaccc ctgtggaaac aaccaaagga ccagaagact ttgaaaccat gtttcgcatt
     2821 aaacaattat cgaccgaatc atggaagaaa atctgaggca tcgttagtga taccgaacta
     2881 cctttatact gtagactgtg cagaaccatt tgaacttctt ttgattgttt ggcatattca
     2941 cccaccaaaa catattgccc catctcagtt aaagacatgt attgattcat caaataaacg
     3001 tcatctatcc catgtacagc ggcaagatac ataaatccat actcgccgct tggtattttt
     3061 tgtgattgtg tggagttcat ctccggtaat tcatcgactt ttggcaagga tggtaatcct
     3121 aatcgatcat cacctaaacc tacactttgt attagagaat cgtcttcgtc aaaatgctca
     3181 taaacattag gctgatcacc tttatggctt atgtacaacc aggatttagt aaatttatcc
     3241 cctgttgcta ttgccttttg gttagcagct ccaataaata aaaatttgct gtcaggagag
     3301 agaactcgct ttagaatatc acttggtgcg tatttgcatc ggtttgcttc aatgaaagcc
     3361 accacttcat tatctaccga ctcaaataat tccaaggcac ataagcctac gctggtccct
     3421 tcaattatta cacggccatc aactgctttg ccataaaggc tttcaaatct tgcaattcca
     3481 tccaaagtag aggttaaaac cacatcgata tcatcagaag ttttttctct aactaaatct
     3541 ttggcaatct gcccagccag agcaaaatcg cctttagcag ttgaattaac acaattagca
     3601 tacaccaaga tt
//
LOCUS       DACTJE010000223         3517 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.225, whole genome shotgun sequence.
ACCESSION   DACTJE010000223 DACTJE010000000
VERSION     DACTJE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.225"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            43..978
                     /locus_tag="I9760_002845"
     CDS             43..978
                     /locus_tag="I9760_002845"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAT5044585.1"
                     /translation="MSPDLAKKLAKRNHLTLPDGLIALDEKSYLSKDFLDFLKVYDTL
                     ASVIKNPQDYYDITFDYLKSNAQEHAIYIEMMYSPDHAEQSSGIPSKEHLAAIQQAID
                     DAKSQFDIVGRIIITAVRHFGAEACERVAKQASIDKFPCVTGFGLGGDEAKFPPQLFS
                     KAYQIAADSGLECTVHAGEFDSAKGMEEAMKTLPIKRIGHGVRVIDSPDIMAMVKDQG
                     IALEVCPTSNIFLGLFKNMNSHPFPKLYEAGIKVSINSDDPPFMSTTLAQEYKRVQKA
                     YGYSDNTMNNITRMAIEAAFVDEKTRKELLTKLEQ"
     gene            1209..2567
                     /gene="gorA"
                     /locus_tag="I9760_002846"
     CDS             1209..2567
                     /gene="gorA"
                     /locus_tag="I9760_002846"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAT5044586.1"
                     /translation="MKTKHFDLIVLGGGSGGIASAVRAAQYGAKVAVIEQNHLGGTCV
                     NLGCVPKKIMYNASSIAETLHKSPDYGFFLENNAKLDWKRLVNKRNAYIERLRENYAK
                     RFSQHKITLIQGKGIFHDQSSITIDHTIYQAEHIIIATGGEPALPAINGIKHAIDSDG
                     FFSLTKLPAKVAVIGSGYIGVELAGILNSLGSETHLLMRGTRPLSRFDHMIGDTLMEI
                     MQKQGICIHQNHKAQAIHLHSDGRKSILCQSGSIIENIDVIISAVGRKPRTGNLNLDK
                     INVNMDDKGLILVDAFQNTSVKGIYAIGDVTNAPALTPVAIAAGRRLADRIFGNQPDA
                     CLNYDNICSVVFSHPPSGSVGLTEHEAIEKYGKSKIKIYQTRFIPMYDALSVDKTPTA
                     MKLVTLGKKEKIIGLHVVGYSADEMLQGFGVAIKMGACKKDFDNTIAIHPTSAEEFVT
                     MV"
     gene            2574..3110
                     /locus_tag="I9760_002847"
     CDS             2574..3110
                     /locus_tag="I9760_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAT5044587.1"
                     /translation="MNYSTRKFEDKYPELGERVYIDPQSTVIGNVTLGDDVSVWPMAV
                     IRGDVNYIQIGHSCSIQDGAVLHVTHDGPYTPGGRPLILGQGITVGHKALLHACTIDD
                     YCLIGMGSIILDSAHIQKHAMIAAGSIVPPGKILKSGHLYLGSPAQAVRKLTTKEIEQ
                     IEYSAGHYIRLKNKYLTY"
     gene            complement(3296..3411)
                     /gene="rrf"
                     /locus_tag="I9760_002848"
     rRNA            complement(3296..3411)
                     /gene="rrf"
                     /locus_tag="I9760_002848"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1133 a          640 c          699 g         1045 t
ORIGIN      
        1 aatttaaaaa aggcagaatt acacgtacat ctagaaggaa ctatttcccc agacctagcc
       61 aaaaaactag caaaaagaaa ccatttaact ctccctgacg gtcttattgc tcttgatgag
      121 aaaagttact tatcaaaaga ctttctcgat tttcttaagg tttatgatac gctggcatcc
      181 gtaattaaaa atccacaaga ttattatgat attacctttg attatcttaa atcaaacgct
      241 caagaacatg ctatctacat tgaaatgatg tactctccgg accatgccga gcaatccagt
      301 ggaattcctt caaaagagca tttagcagca attcaacagg ctattgatga tgctaaaagt
      361 caatttgata ttgttggacg tattattatc actgcagtac gccattttgg cgcagaagca
      421 tgcgaacgcg tcgcaaaaca agcttctata gataaatttc cttgtgtaac aggatttgga
      481 ttaggaggag atgaggcaaa atttcctcct caattgtttt ccaaagctta tcagatagca
      541 gcagactccg gattggaatg caccgttcat gccggcgaat ttgattcagc caaaggaatg
      601 gaagaagcaa tgaaaactct gccaattaaa agaattggac atggggttcg agtgattgac
      661 tcacctgaca tcatggccat ggttaaagac cagggtattg cattggaagt gtgcccaacc
      721 agtaacatat ttttgggatt attcaaaaac atgaatagcc acccttttcc aaagctttat
      781 gaggcaggga ttaaagtcag tattaattcc gatgatcctc cttttatgag cactacactt
      841 gctcaagagt acaaaagagt tcaaaaagct tatggctata gcgataacac aatgaataat
      901 attacgcgta tggctattga agctgctttt gttgatgaaa aaacccggaa ggaactttta
      961 acaaaattgg aacaataatc tcagtcttat attcctgttg ccagtactaa tttcatgctg
     1021 gtttgaagct aaaacgatac tgttatattt ttacaggttg gtgttgatat atggcttaaa
     1081 caccaaccat aactaaaacc atctggttga aattaaaaaa tattttggtt ttttataaaa
     1141 ttcagtttaa tcccttggta gaccaagtac gacctgctac tattatttta ttttcctatt
     1201 aattgactat gaaaacaaaa cattttgacc ttatagttct tggaggagga agtggcggaa
     1261 tagccagtgc tgttcgtgct gctcagtatg gagccaaagt ggcggttatt gagcaaaacc
     1321 acttgggagg gacctgcgtg aatttaggat gtgtccccaa aaaaattatg tacaatgctt
     1381 catcaattgc agaaacactc cataaatctc ctgattacgg ttttttcctg gaaaataatg
     1441 ccaagttaga ttggaagcga ctagtgaata aacgcaatgc gtatattgaa cgtttaagag
     1501 aaaattatgc gaaacgattt tcacagcaca aaattactct gattcaaggt aagggtattt
     1561 tccacgatca aagttctatc acgatagatc atactattta tcaagcagaa catattatta
     1621 ttgcaactgg cggtgagcca gctctaccag caattaatgg cattaagcat gctattgatt
     1681 ctgatggttt tttttcatta accaaattac cagctaaggt agctgttatt ggtagcggtt
     1741 acataggtgt tgaattagca ggaatcctta actctcttgg tagtgaaact cacttgttaa
     1801 tgagaggtac acgaccactg agtcgctttg atcatatgat tggcgatact ttaatggaaa
     1861 ttatgcaaaa acaaggaatc tgtattcatc aaaaccataa agcccaagct atccatctcc
     1921 atagcgacgg cagaaaaagt attttgtgtc aaagtggctc tattattgaa aatattgatg
     1981 tcattatatc tgctgtaggt cgaaaaccgc ggactggcaa tttaaatctt gataaaataa
     2041 acgttaacat ggatgacaaa ggattaatcc tggttgatgc attccaaaat acatcggtta
     2101 aaggtattta tgctattggc gatgtcacta atgctcccgc actaactccc gttgcaatag
     2161 cggcagggag gcgtttggca gaccgaattt tcgggaatca acctgatgcc tgcttaaatt
     2221 atgataatat ttgttcagta gtattctctc atcctccatc tggttctgtc ggtttaaccg
     2281 agcatgaagc cattgaaaaa tatggtaaga gtaaaattaa aatatatcaa acccgtttca
     2341 ttcctatgta tgatgccttg agtgttgata aaacccctac tgcgatgaaa ttggttacct
     2401 tgggtaaaaa ggaaaagatt attggtttac atgtcgtcgg atacagcgcc gatgaaatgc
     2461 tacaaggctt tggggttgcc ataaaaatgg gcgcatgtaa aaaggacttc gataatacaa
     2521 ttgcaattca tccaaccagt gctgaagaat ttgtaactat ggtgtaaata tacatgaact
     2581 attccactcg taaatttgaa gacaagtatc ctgaattagg tgaacgagtt tatattgatc
     2641 cgcaatccac tgtcattggt aacgtaaccc ttggagatga cgtatccgta tggcctatgg
     2701 ctgttatccg tggtgatgtt aattacattc aaataggcca ttcctgcagt attcaagacg
     2761 gtgctgttct gcatgttact catgatggtc cttatactcc aggcggtagg ccattaattt
     2821 tagggcaagg aatcacggta gggcacaaag ctctattaca cgcctgtact atcgatgatt
     2881 actgtttaat tggcatggga tctataattt tagactcagc gcacatacag aaacatgcta
     2941 tgattgctgc aggaagcatc gtacctccgg gaaaaatact taaaagtggg catttgtatt
     3001 tgggaagccc tgctcaagcc gtcagaaaat taaccacaaa agagatagag caaattgagt
     3061 attctgcagg gcattatata aggctaaaga ataaatatct tacatattag gttactagct
     3121 actcattttt cttatccaca tgttactttt gataaattaa aaaaggaaat caagccattt
     3181 aaactcaact tatgacttaa agagccagtt aagtaacgag tattttatgt agaaaagacg
     3241 gtgtaattta ttttataaaa aaaaagcggc ttgtgagccg ctataaggaa taaaaccctg
     3301 gcgatgacct actttcgcat gaggaagcct cacactatca ttggcgcgga aatgtttcac
     3361 ttctgagttc gagatggtat caggtggttc caaatcgcta tagtcgccag gaaaactggt
     3421 ttacctgagg ctttgattgc tgtattaagc cacaggtttg gtaaagagtc agagtttaca
     3481 cgatacgttt cacgtataca aatcaatatc ataatct
//
LOCUS       DACTJE010000224         3466 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.226, whole genome shotgun sequence.
ACCESSION   DACTJE010000224 DACTJE010000000
VERSION     DACTJE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.226"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1679
                     /locus_tag="I9760_002849"
     CDS             <1..1679
                     /locus_tag="I9760_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAT5044588.1"
                     /translation="SGILGLSLLSLACGSFAMTETETIRVIIKYKQQPATIASLKSQV
                     IKSVQIPVKEMKPMANGAYSLIFDTSNSHAIVKSKEEDVTAKVLERLRKNPNVLYAVK
                     DRIGHFKPLPDPVINQELSQLLSHESQWDEFERPAGIRLESRPGLRDGAWAYTTGKSR
                     KPIVVAVLDTGIALNDSLVNNLVKDNEGNVFGWNFAGNNNDLSDETRSYHGTHVAGTI
                     AGYGDVMNGVGEDLKILTVKIPAENGMFYESAVVNSIYWAVGGDVPGVPKNIHPAKVL
                     NMSFGVDLEPGKEIDYCDEALQEALFFARKKGAVVAVAAGNDNVWEHYNAPAVCNGTI
                     KVASTGPEGLRSYFSNYGPSITLAAPGGDKRYGTSGGILSTVNPGGGYNSSGFDFYQG
                     TSMASPHVAGVAGLIFAVSNKALKPEQVEQILYVTTHEFGQSNDTNKSCVGKKPCGHG
                     ILDADNAVQAALNAYDVLFSAPKMIALATHECGENRLKPGKNIINDSTATWIQAKSSC
                     ETGVSYQKPRIEKAKDGKIYAYYGSVSYELDQSAYKECRVVGYDGVGCYL"
     gene            complement(1832..2113)
                     /locus_tag="I9760_002850"
     CDS             complement(1832..2113)
                     /locus_tag="I9760_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044589.1"
                     /translation="MAETIDDITIAFNENGVETTRELDKQILSKGAWTTIMFKYQDWD
                     NTKNDYGPVKYSIRRYQKRNNQYWQKSKFNISSTEQAKKIVDILNNWLE"
     gene            complement(2127..2741)
                     /locus_tag="I9760_002851"
     CDS             complement(2127..2741)
                     /locus_tag="I9760_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HAT5044590.1"
                     /translation="MEKAMKSILLMSLLLLIMPFRLMSAQSLYCPQNHGYINIGMSMD
                     QVIAACGQPLSKQESNQPILQKIPVQQLIYNNLGNSDGLYSGSLNVPEGTAFYGVWNI
                     PTGSSGVQLEVDILNNKVQDIRVNGGNTNAFSLCGNASIQIGDPATKVYGACGSPNLV
                     NNTYINQVVPTETKPQIWIYKPGPYQPAFSLTFVDGKLQSIGNN"
     gene            complement(2789..3364)
                     /locus_tag="I9760_002852"
     CDS             complement(2789..3364)
                     /locus_tag="I9760_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAT5044591.1"
                     /translation="MAGLTAVGYFLLSLLFTIVIFSLWLRIAIRFFRISTINPFSQLI
                     HTITDPLVKPLYWIIKKPLQPGQKYDWIAFGVLILVEFFKIISLSLLMYHAIIPFLFI
                     FLYILVDLIIQPCEILFFAVLIRVIMSYVNPKWQHPLGDFLRVITTPILRLGRIIVPD
                     ISGFDFSPFIIMVILKIITLFLSASLPWKLL"
BASE COUNT         1082 a          595 c          721 g         1068 t
ORIGIN      
        1 aatcaggaat attgggatta agtcttttat cgcttgcttg tggcagtttt gctatgactg
       61 aaactgaaac aatcagggtt atcattaaat ataaacagca acccgccact attgcttcac
      121 tgaaatcaca agtcattaaa tctgttcaaa tacccgttaa agaaatgaag cctatggcca
      181 atggcgctta ttcgcttatt tttgatacat cgaattccca tgctattgtc aagtctaaag
      241 aagaagatgt tacagccaaa gttttagaac gtttgcgcaa aaatcccaat gtactttatg
      301 cagttaaaga cagaatagga cattttaagc ctctccctga tccagtaata aaccaggagt
      361 taagtcaatt gttatcccat gagagtcaat gggatgaatt cgaaaggcca gcgggaattc
      421 gtttggagtc caggcctggt ttaagagatg gcgcatgggc atatactaca ggaaagtcta
      481 ggaagccgat agttgttgcg gttttagata ctgggattgc attgaatgac agtttggtga
      541 ataaccttgt aaaagataac gaaggtaatg tatttggatg gaattttgcc gggaataaca
      601 atgatttatc tgatgaaacc agatcttacc atggcaccca tgttgccggt accattgccg
      661 gttatggcga tgtcatgaac ggagttggtg aggatttaaa aattctgact gttaaaattc
      721 ctgccgaaaa tggtatgttt tatgaaagtg ctgtggtgaa tagtatctat tgggctgttg
      781 gaggtgatgt tccgggagtt cctaaaaata tacatccagc caaggtatta aatatgagtt
      841 ttggtgtcga tttagagcct ggaaaagaaa ttgattattg cgacgaagct ttacaagagg
      901 ctttattctt tgctcgcaag aaaggggctg tagtcgcggt tgctgccgga aatgataatg
      961 tttgggaaca ctataatgct cctgcagtgt gcaatggaac gatcaaggtt gcttccaccg
     1021 gaccagaagg attgcgttcg tacttttcaa attatgggcc cagtataacc cttgcagcac
     1081 cgggaggtga taagcgttat ggcacttcgg gaggtatttt atctaccgta aacccgggag
     1141 gtggatacaa ttcatcaggc tttgattttt atcaagggac gagtatggct tcgcctcatg
     1201 tagccggggt agcaggttta atttttgctg tcagtaataa ggctttgaaa cctgaacaag
     1261 ttgaacaaat tttatatgtt actacgcatg agtttgggca aagcaacgac accaataaat
     1321 cttgtgtcgg caaaaaacct tgtggacatg gaatattgga tgcggataat gctgtgcagg
     1381 cagcattaaa tgcttatgat gtcttattct cagcgcctaa aatgattgct ctggcaacgc
     1441 atgaatgtgg agagaataga ttaaaacctg gtaaaaacat cattaatgac agtactgcga
     1501 cctggattca agctaagtca agctgtgaaa ctggtgtaag ctatcagaag ccacgtattg
     1561 aaaaagccaa ggatggaaag atttatgctt attatggttc cgtaagctat gaattggatc
     1621 aatcggctta taaagaatgt cgtgtcgttg gatatgatgg cgtaggctgt tacttataat
     1681 atcaaatgaa acttgattct cagttttaag acgttttgat gagggaagta ttcagagcca
     1741 atttattggt tacgtatgga atccttctct catcaaatta aaatccctgc attcgcgcag
     1801 gtattaaaat tttaacaaag acatcagaaa attactcaag ccaattattt aatatatcaa
     1861 caattttttt ggcttgttca gtactcgaaa tattgaattt ggatttttgc caatattgat
     1921 tgttacgttt ctgataacgc cgtatagaat attttacagg accgtaatca ttttttgtgt
     1981 tatcccaatc ctggtattta aacataatag tagtccatgc tcctttggat aggatttgtt
     2041 tatcgagctc cctggttgtt tccactccat tttcgttaaa agcaattgtt atatcatcta
     2101 tggtttcagc catttgtcat acttcctcaa ttatttccga tggattgtaa tttgccgtcg
     2161 acaaaagtca aggaaaaggc tggttgatat ggacctggtt tataaatcca gatttgtggt
     2221 tttgtctctg taggaaccac ttggttaatg tacgtgttat tgaccaggtt cggactgccg
     2281 caagcgccat aaactttagt ggcaggatct ccgatttgaa tgcttgcgtt gccacacaag
     2341 gaaaaagcgt tagtattgcc gccattcact cgaatatcct ggactttatt attgagaata
     2401 tcgacttcca attgcacgcc gctactacct gtaggtatat tccatacgcc ataaaaagcg
     2461 gtaccttccg gtacatttaa tgatcctgaa tacaggccat cggagtttcc cagattatta
     2521 taaattagtt gctgcacagg gattttctgc aaaataggct gattggactc ttgtttactc
     2581 agtggttgcc cgcacgcagc aatgacctga tccatggaca taccgatatt gatataacca
     2641 tgattctgag ggcaatacaa agattgtgcg ctcattaaac ggaatggcat aattagcaat
     2701 aataaactca taagtaatat cgatttcata gctttttcca ttaatccgta tcaactaata
     2761 gtatagttct gtttgtttat ttttgctttt ataataactt ccagggcaaa gaagcgctta
     2821 gaaacagggt aataattttt aatatcacca tgataataaa gggtgagaaa tcaaaacctg
     2881 aaatgtccgg gactataatt ctgcctagtc gtaaaattgg tgttgttatt acgcgcaaga
     2941 aatcaccaag cggatgttgc catttgggat taacataact cattattact cgaatcagaa
     3001 cagcaaaaaa taatatttcg caaggttgaa ttatcaaatc aacaagtatg tagagaaaaa
     3061 taaagagaaa tggaatgatt gcatgataca tcagtaaact cagtgaaatt attttaaaaa
     3121 attccaccag gattaaaact ccaaatgcta tccagtcgta tttttgtcct ggttgcaatg
     3181 gcttttttat aatccaatac aagggtttca ctagaggatc agtaatggta tggattaatt
     3241 gagagaatgg attaatagta ctgatacgaa aaaaacgtat agcgattcta agccacaaac
     3301 tgaaaataac gatagtaaat aataaggata ataaaaaata cccgacagca gttaaaccag
     3361 ccattgtttt acctctttag attgtattca tcaataaagc cagcaaattt attcatatga
     3421 tttagaaatg aattgcttaa gcagtttatt aaccttctat cataac
//
LOCUS       DACTJE010000225         3414 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.227, whole genome shotgun sequence.
ACCESSION   DACTJE010000225 DACTJE010000000
VERSION     DACTJE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.227"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            433..1563
                     /locus_tag="I9760_002853"
     CDS             433..1563
                     /locus_tag="I9760_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HAT5044592.1"
                     /translation="MKEKYIGLHKIELNTPCLVIDYEKLKYNLKVMQQHGFEWGIGIR
                     PHCKTHKCTQIARLQLEYGAIGISAAKLSEAEVLVSKQITNVLITSPIVSEAKYSRLF
                     NCLKSAPDLMLVVDGKDNIRMLNELGKSLNQPVYVLLDIDSGIGRTGINPEVALEYGH
                     FINSMNWLKLKGIQCYAGHLQHIQNYEERKAFSLRIMDMASRIVKSFRDANLPCEILT
                     GTGTGTFDIDVEASEVTEIQPGSYAVMDVEYGSIGSKNNPSSFQPFKNSMTLLTSVIS
                     SNRKEHATVDAGTKSIYVDPHHKPHIISHPGLVYDWGGFGDEHGKVFSCEGGSHFLQN
                     GDVLELIVPHCDPTINLYDKFYITENNVVVDIWDIDMRGKSQ"
     gene            complement(1604..1783)
                     /locus_tag="I9760_002854"
     CDS             complement(1604..1783)
                     /locus_tag="I9760_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003635698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAT5044593.1"
                     /translation="MLSWALIFFIIAIIAAAFGFGGIAVAAAGIAKILFFLFLVMFVI
                     FLIMGLLGRRGPPPV"
     gene            1874..2242
                     /locus_tag="I9760_002855"
     CDS             1874..2242
                     /locus_tag="I9760_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044594.1"
                     /translation="MQTTKAHYSKIFFALFLFFCTGSSALFACPCFNMGFLHVAFLDD
                     KDTECRIYKEGSVVYKIEIFDKEKIASSTHTQCTLNTEHHDVFMNYDFHYLDDHGDCI
                     REILDACNKLKINSLHMDDE"
     gene            complement(2335..2895)
                     /locus_tag="I9760_002856"
     CDS             complement(2335..2895)
                     /locus_tag="I9760_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044595.1"
                     /translation="MLILIQKGKNMSKFPHKTPETLRQYFQAKDLGQLTEINHSYGPH
                     FENLENAIDRLTQEISTQKGKLSGFLKEQEKISQNYGKIVIEQERFENNRASIMSDSC
                     TGAERYLALAALGMSPFDCYYSNSRSLQDQIDTTNKKLNELNSHFAFWKNQRSKAVSE
                     LKILNSIIDEKRKELEVDHQFTLSSN"
     gene            complement(3086..>3414)
                     /locus_tag="I9760_002857"
     CDS             complement(3086..>3414)
                     /locus_tag="I9760_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044596.1"
                     /translation="SLGQKYGSFFWGCTKFAVSPYTNTFLSIVKSKEVEEADCLSKAL
                     AASIVCGVLTFVVPVLPVLCAFTFTLSSIAMLLAVASMFVTYPIAILGDALCGPELSD
                     DYHYSF"
BASE COUNT         1112 a          556 c          662 g         1084 t
ORIGIN      
        1 aatgaggtga catgtcgcat aagcgacatt tgtgtattta aattttcagg acatttgttt
       61 tctaatctat tagttcaaat tttgaatatt gagaaagata agaactaaat atgttttggg
      121 ttatcaaggg gataaaattt tgtttgggag aggtaaatat cctgtgataa gtgtcctaag
      181 attatttttt aatcacaaaa tagaatgatt tgaatcagag aaaaatggtg gttgatgtaa
      241 gaaataaata actcatcaat tataaaatca ttgaaatcga ggagccagct catagcagtt
      301 agtcatgcaa ttttatattg atctttgagg attaggatgc tcatctttta aaaaatagtt
      361 taataaaagc ttaaattcct aagcaattta tgtcataaaa cgcatttatt gaactttgtg
      421 atgaattatc tcatgaaaga aaaatacatc gggcttcata agatagaact aaatacccca
      481 tgccttgtga ttgattatga aaagctgaaa tacaacttaa aagtgatgca acaacatggt
      541 ttcgaatggg gcataggaat aagaccgcat tgtaaaactc ataaatgtac tcaaatcgca
      601 cgtttacaac ttgaatatgg ggcaataggg atttctgccg ccaaactatc agaggctgaa
      661 gtattggtat ccaaacaaat cacgaatgtc ttaattactt cacccattgt atcggaagcc
      721 aagtattcaa gattatttaa ttgtcttaaa tcagcgcctg atttaatgct ggttgtagat
      781 ggcaaggata atattcgcat gcttaatgag cttggaaagt cgctaaacca gccagtttat
      841 gtcttgcttg atatcgattc aggcattggt cgaacgggca ttaatcccga ggtagctttg
      901 gaatatggtc attttatcaa tagtatgaat tggcttaagc taaaaggaat tcaatgttat
      961 gcaggccact tgcaacacat tcaaaactat gaagagagaa aagcgttttc tctacgtatt
     1021 atggatatgg ccagcaggat tgttaagagt tttagagatg ccaatctccc ttgtgaaata
     1081 ttaactggca ccggaactgg tacttttgat attgatgtcg aagcaagtga agtaacagaa
     1141 atccagcctg gctcatatgc tgtaatggat gttgagtatg gttcaattgg ttcaaaaaat
     1201 aacccatcaa gctttcagcc atttaaaaac tcgatgactc ttttaacaag tgttataagc
     1261 agtaatcgta aagagcacgc tactgttgat gctggaacga aatcgattta tgtcgatcct
     1321 catcataaac cacatattat tagtcatccc ggactggttt atgattgggg tgggtttgga
     1381 gatgagcatg gcaaggtatt ttcttgtgaa ggcggaagtc atttcctgca aaatggcgat
     1441 gttttagagc ttatcgttcc tcattgtgat ccgacaataa acttgtatga taagttttat
     1501 atcacagaaa ataatgttgt tgtcgatatt tgggatatcg acatgcgtgg aaaatcacaa
     1561 taatctatac tatttaacca ggtgtttgag cttcatattt ttgctagaca ggaggagggc
     1621 cacgacgccc gagtaatccc ataataagaa atatgacaaa catcaccaaa aatagaaaga
     1681 ataatatttt tgcaattcca gcagcagcca ctgcaatgcc gccaaatcca aacgcggctg
     1741 caataattgc aatgataaaa aaaataagtg cccaacttaa cataacagct ccctgtaaag
     1801 attatcctgt tcattaaatt atagactaat aatggtctac aattagacaa aagacagtta
     1861 atcagggaag aatatgcaaa cgactaaagc tcactatagc aagatattct ttgccttatt
     1921 tctttttttc tgcactggtt caagcgcatt atttgcctgc ccttgtttta acatgggctt
     1981 tttacacgta gcctttttgg atgataaaga tacggaatgt cgcatatata aagaaggaag
     2041 tgtcgtttat aagatcgaga tttttgataa ggaaaaaatt gcgtcaagta cccataccca
     2101 atgtacgctg aatacggagc atcatgatgt ttttatgaat tatgattttc attacctcga
     2161 tgatcatggg gattgtatca gagaaatatt ggacgcatgt aacaaactga aaataaactc
     2221 tttgcatatg gatgatgagt aagataaagc aaggtgaaat aggactggta ttcaatgatt
     2281 aggacgctcg cccggattat aattaagatt aacccgggtt aggcttatca atttttagtt
     2341 tgaactcaat gtaaattgat gatcaacttc aagctctttt cttttttcat cgataatact
     2401 atttaatatc tttaattcag aaaccgcttt cgatctttga tttttccaaa aagcaaaatg
     2461 agaatttaat tcatttaatt ttttgtttgt cgtatcgatt tgatcttgta aactcctgct
     2521 attgctatag tagcaatcga aaggcgacat tccaagtgcg gcgagtgcta aatagcgttc
     2581 tgctccggta caagagtcgc tcataatcga tgcacgattg ttttcaaatc gttcttgttc
     2641 aattacgatt tttccataat tttgggaaat cttttcctgt tcttttaaaa atccggatag
     2701 ttttcctttt tgagtggata tctcttgagt cagtctatca atcgcgtttt ccagattttc
     2761 aaaatgaggc ccataggaat gattaatttc tgttaattgc cccagatctt ttgcttggaa
     2821 atattgacga agtgtctcgg gggttttgtg agggaattta gacatgtttt tacctttttg
     2881 aatcaaaatc agcatatctt agcatatgct ttttgatcat atcaaacata tatgctgcat
     2941 aatgagcaaa ttaatggctt ttgttgatgg ctgggttcat tttttctcaa tatcaaacag
     3001 gaagagtatt ttttacgagt ttaaaaatac tcttcctgtt tgttgatact tatgatgatt
     3061 tcaaatgaaa ttatcttaaa actgattaga aagaataatg gtaatcgtca gacaattctg
     3121 gtccgcacag tgcatcacca agtattgcaa tcggataagt cacgaacatg ctggctaccg
     3181 caagcaacat ggctattgaa gacagggtaa acgtaaaagc gcatagaact ggtaataccg
     3241 gaacaacaaa agtgagaact ccgcagacaa tgctggcagc aagagccttg gataaacaat
     3301 ctgcttcttc aacctctttc gatttgacga tggatagaaa agtattggta tagggggaaa
     3361 cagcaaattt agtacatccc caaaagaatg agccgtattt ttgacctaac gaga
//
LOCUS       DACTJE010000226         3399 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.228, whole genome shotgun sequence.
ACCESSION   DACTJE010000226 DACTJE010000000
VERSION     DACTJE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.228"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..421
                     /locus_tag="I9760_002858"
     CDS             <1..421
                     /locus_tag="I9760_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAT5044597.1"
                     /translation="FLHFLKLNRSVPENVLIVNYIVDNIPYVHYSQRYEIVCLDEKVC
                     KLVIHYGFMETINIPRSLEKACNKNILPFKFNVDTATFMVEIPNIMASKEKRSLSFYW
                     QEKLFAFLMRNYSANLNIEFYKLPYNRTIAIGTYCIL"
     gene            539..1477
                     /locus_tag="I9760_002859"
     CDS             539..1477
                     /locus_tag="I9760_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAT5044598.1"
                     /translation="MWFNNALMFQYKLDGGCDLNHSLTEEALKPCPPHARFTYGWVPA
                     FEQEMVHEVAGSTLICLGKEERILPRGVINKMLKEKIQQIEAAQGRPVKRAEKAQMAE
                     DLEFELLPKSFCVQKKLLAILDSVSQRLIINTSSNNQASQLTSFLRKSIPGIVIEPLN
                     LTENLALRFAEWIHSPATLPDDFQLASDCLLFSLEDEKKRVHCKGYELPAEEILTLLA
                     QGMGTAEISLIWKERIQFTLTQDFTFKKLKSLDYLIDDFNEIRQLEEEYQRQDAALTL
                     LSGELRELSNGLMKIFVNHAEMKSAQIEKVAEDCTI"
     gene            complement(1625..2398)
                     /locus_tag="I9760_002860"
     CDS             complement(1625..2398)
                     /locus_tag="I9760_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAT5044599.1"
                     /translation="MKRIHVLFLIGLVVGISSCTKWGKDDEDNNPYKGMTAKQLYTAA
                     QTSLKKEEYATAAKQLEAMESMYPFSDYTESSQMQLIYAYYKDEDYPSAAATAERFIH
                     LYPRAKNVDYAYYMRGLANFQQTRGVFAKMLPMDESWRDPGTQTQALVDFATLIQKFP
                     DSKYKANALQRMIYLRNMFAQHELNVSLYYFKRKMYVAAIERASYLVKNYPQAPSAQK
                     ALVVMYEANKALGLNKAAEDAMAVYKATYHTSNMIRINS"
     gene            2564..2651
                     /locus_tag="I9760_002861"
     tRNA            2564..2651
                     /locus_tag="I9760_002861"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2598..2600,aa:Ser,seq:tga)
     gene            complement(2782..>3399)
                     /locus_tag="I9760_002862"
     CDS             complement(2782..>3399)
                     /locus_tag="I9760_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044600.1"
                     /translation="SIAESNEFFNIKRLILNLLRAYQNPWHAFFSRTHLGVGNYEQDI
                     RNCQSNSDLLAVLNHMERNLGKRNPHGHMQSLIYYAKWVIDFSNKYQHETLMKFKEYF
                     ACQQIRKLADQEIHHAGLIVGFGDRQAKQQRFKVLSNELADLPEDVAEYRKAIDNWLA
                     TRVNDGQSTQQLLSENRSSVFAKKVTRGESTVLHIKTTLDLAVSS"
BASE COUNT         1050 a          655 c          641 g         1053 t
ORIGIN      
        1 ctttctccat tttcttaaac taaaccgctc agtaccggaa aatgtcttaa ttgtaaatta
       61 tattgttgat aacattcctt atgttcatta tagccagcgc tatgaaattg tttgccttga
      121 tgaaaaagta tgtaaactag taatacatta tggctttatg gaaacaatta atatcccaag
      181 aagtctggaa aaagcttgca ataaaaatat acttcctttt aagttcaatg ttgacacagc
      241 aacatttatg gttgaaatac ccaatattat ggcttcgaaa gaaaaaagat cactaagttt
      301 ttattggcaa gaaaaactgt ttgctttctt gatgcgcaat tactcagcga atttaaatat
      361 tgaattctat aaattacctt acaatagaac tatagcaata ggtacctatt gtattttatg
      421 agttttggtc agggtctgtt ggcagtttgc tctcttgcgt cgaaatggca acagccccta
      481 atcatcgcag taaaatgcta tccgtgataa catcgcaaat ttattttaag gactgattat
      541 gtggttcaat aatgcactga tgtttcaata caaactagac ggtggatgtg atttaaacca
      601 ttctttgact gaagaagcgc ttaagccctg ccccccccat gctcgcttta cttatggctg
      661 ggttcctgcg tttgagcagg aaatggtgca tgaagtagct ggtagcacgc ttatttgcct
      721 gggtaaagaa gagcgaattc tccctagagg agtcattaat aaaatgctca aagaaaaaat
      781 tcagcaaata gaagcagccc agggtcgacc ggtaaaacgc gcagaaaagg ctcaaatggc
      841 tgaggatctt gaatttgaac tattgcctaa atccttctgt gttcaaaaaa aacttttagc
      901 gattcttgat agtgtcagtc agagattaat tattaatact tcaagcaaca atcaggcgtc
      961 tcagttgact tcttttttgc gtaaatcaat ccctggtatt gtgatagaac cattaaacct
     1021 taccgaaaat cttgccttgc gattcgcaga atggatccat tctccagcaa ctctacctga
     1081 tgactttcaa ttagcctccg attgtttact cttttctctt gaagatgaaa aaaaacgagt
     1141 ccattgtaaa ggctacgaat tacctgccga agaaatcctg actctattgg ctcaaggtat
     1201 ggggactgca gaaatctcat taatttggaa agagcggatt cagttcacct tgacgcagga
     1261 ttttactttt aaaaaactga aaagccttga ttatctgatc gacgatttta atgagatcag
     1321 acaattggaa gaagagtatc aaagacaaga tgccgcgttg accttattaa gtggggagtt
     1381 aagagaactt agtaacggat taatgaagat atttgttaat catgcagaaa tgaaatcggc
     1441 tcaaattgaa aaagttgcag aagattgcac aatataaagt cgtatcattt taacccgtaa
     1501 cctatcgagg gagctccttt gctaacagag ctccctctca tcttgattca agagaaaacg
     1561 agtggattgc acatcacttg cttaacccag ctgacagaat aaactctata aatttttgta
     1621 ctcatcaaga attaattctg atcatattag aagtatgata agtagccttg taaacagcca
     1681 tagcatcctc cgctgccttg ttaagcccta atgctttatt ggcttcgtac attaccacta
     1741 aagccttttg agcgctggga gcttgaggat agtttttcac tagataactg gcccgttcaa
     1801 tggcagccac atacatttta cgtttgaagt agtaaagaga gacattgagt tcgtgctgag
     1861 caaacatatt tcgtagataa atcatacgtt gcaaagcatt ggctttatat ttgctgtcag
     1921 gaaatttctg aattaaagta gcaaagtcaa ccagtgcttg agtttgtgtt ccagggtccc
     1981 tccaggattc atccatagga agcattttgg caaatacgcc tcgtgtttgc tggaagttag
     2041 ccaaacctct catataatag gcataatcaa cgttttttgc gcgtgggtac agatgaataa
     2101 agcgctccgc cgttgctgca gcagaaggat aatcttcgtc tttataatag gcataaataa
     2161 gctgcatttg agagctttca gtatagtcac taaaaggata catagactcc atggcttcca
     2221 actgtttggc agcagtagca tattcctctt tttttaaaga ggtttgtgcc gcagtgtaga
     2281 gctgtttagc tgtcatgcct ttatatgggt tattatcctc atcatctttt ccccatttag
     2341 tacacgatga gattccaaca actaacccaa taagaaataa cacatgaatt cgtttcataa
     2401 agcacaccta tcaattatat ctgctgtatt tataagtaca gcaactggaa aatttgggac
     2461 attataccta ggaccagtta atttgcgaac aacctattat tcttttattt atctataaaa
     2521 aaacatttgc aaaatgatag gcgttcatat agtattctcg accggagaga tggcagagcg
     2581 gtcgattgcg gcggtcttga aaaccgttga agggcaacct tcccagggtt cgaatccctg
     2641 tctctccgcc agacaacatt accatggtat atttccccga gacaaccaat ttcactttag
     2701 cgttattcta ttcttcaagt acaaaaagta agtgaatgac atccaaccca ttaatacaaa
     2761 agactaaagt cattgtatct attaacttga tactgccaga tcaagagttg ttttgatatg
     2821 gagcacggtg ctttctccac gggttacttt ttttgcaaaa acagatgagc gattttcact
     2881 aagcaattgt tgggtagatt gtccatcatt aactcgtgtg gcaagccaat tatcgatagc
     2941 ttttctatat tctgcaacat cctcaggcaa atcagccaat tcgtttgaaa gtactttaaa
     3001 ccgctgttgc ttcgcttggc gatctccgaa acccactata aggccagcat gatgaatctc
     3061 ttgatcagcc aattttctta tctgttggca agcaaaatat tctttaaatt tcattagagt
     3121 ttcatgttga tatttattgg aaaaatcaat tacccattta gcataataaa tcaaactctg
     3181 catgtggcca tgtggatttc ttttgcctag atttctttcc atatggttta atacagcgag
     3241 taaatcgcta ttactctggc aattgcggat atcttgctcg taattaccta ctccaagatg
     3301 cgttcgggaa aaaaaggcat gccagggatt ttgataggct cgcaacagat ttaaaataag
     3361 tcgtttgata ttaaagaatt cattcgactc agcaatgga
//
LOCUS       DACTJE010000227         3332 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.229, whole genome shotgun sequence.
ACCESSION   DACTJE010000227 DACTJE010000000
VERSION     DACTJE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.229"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..499
                     /locus_tag="I9760_002863"
     CDS             <1..499
                     /locus_tag="I9760_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAT5044601.1"
                     /translation="YKFFGIYTATAVAMVASLTQVAFYRLKFQHYEKMHLFSLAIIMV
                     LGGATLFFQNPWFIKWKPTGIYWLSALVFYGSSYIGSKPLIQKMMEANINLTTKIWYR
                     LNLAWTLFFIVMGALNLYVAYHYDTDVWVNFKLFGGVGFTLLFVLIQAFYLTKHTDEK
                     SFEKQ"
     gene            560..1237
                     /locus_tag="I9760_002864"
     CDS             560..1237
                     /locus_tag="I9760_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT5044602.1"
                     /translation="MRLLLVEDDELLGDAVKAGLTQFGYVVDWLKDGEAARAALKSES
                     FELIILDLGLPKLSGLSLLQNIRHDGNSTPVIILTARESIEDRVKGLDSGADDYLIKP
                     FDLNELSARIRALVRRSQGRADSVLQYKNITLDPAAHSVFVDDVLVNVPRREFALLQK
                     LLENSGQVLSREQLMQSIYGWEEDVDSNALEVHIHNLRKKLNANFIRTIRGVGYMAEK
                     NDSVLLS"
     gene            1234..2649
                     /locus_tag="I9760_002865"
     CDS             1234..2649
                     /locus_tag="I9760_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAT5044603.1"
                     /translation="MKSSIRKFLLINLLLAITITTTLTAIGNYYLDQKDIQDHLDTLM
                     AISALSYQALLGDDLHQRPLAKIQNALESIPQKINTYYQKRYLNDEPQENYLDKFNFQ
                     VWTNGGKLLLHSPTAPKIPLTAETDGFSDKRISNQDWRVFTTYNDKAGIRTVLAERYD
                     TRNELGHRIAQDDLYIMLLTFPLSGLLIWIIIGRGLDSLDRVAEEVANRAPSHLEPVD
                     LQEVPEEIKPVIDELNKLFFRLKEGFEREKRFAADAAHELRTPLAALKAQAQVALNSN
                     DIEEKNQALQKLIASVNRSTHIVQQLLTMSKLVPEATELHEQDEVNLSKLTREILAML
                     APSAVEKQIELEFETDEKMPKIYGNPTALGILIRNLVDNSIRYSMENGRVIVRLLKHN
                     NEIILEVCDNGPGIPPELQKRVFERFFRVLGNKSPGSGLGLAIVQQICELHGGRVVLD
                     SPKIGTGLIVRVYLPIKTKPK"
     gene            2933..>3332
                     /gene="lem8"
                     /locus_tag="I9760_002866"
     CDS             2933..>3332
                     /gene="lem8"
                     /locus_tag="I9760_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem8"
                     /protein_id="HAT5044604.1"
                     /translation="MPQILNALAAVIEADLVKLNPEQKEIVDKYSDKLLNVQYKAHQP
                     RTFELAVKTMKEQLEQLSTSLKKINSEMAPDKDKRRHEMIQKIESDLKLIESCINGDF
                     EKNYQNLKKEFLQFLHEADSKDPFLKKIHGL"
BASE COUNT         1142 a          563 c          603 g         1024 t
ORIGIN      
        1 ctataaattt tttggcattt ataccgctac tgctgttgct atggtagctt cattaactca
       61 agttgcgttt tacagattaa agtttcagca ttatgaaaaa atgcatttgt ttagtctggc
      121 tatcattatg gtcttgggag gagcgacgtt attctttcaa aacccttggt ttatcaaatg
      181 gaagcctaca ggaatctact ggttatctgc tcttgtcttt tatggttcaa gttatatagg
      241 atctaagcca ctaatacaaa aaatgatgga agcaaacatc aatttaacaa caaaaatttg
      301 gtatcgactt aatttagcat ggacattatt ttttatagta atgggggcat taaatttata
      361 tgttgcctat cattatgaca ctgatgtatg ggttaatttt aaattatttg gtggtgttgg
      421 gtttacttta ctttttgtgt tgattcaagc tttttattta acaaaacaca cagatgaaaa
      481 aagctttgaa aaacaatagt gatatctctg tatctgcaga aaaatatagc atgtaataag
      541 agaaaaagta ggagcaccga tgagattatt acttgttgaa gacgatgaac tgcttggcga
      601 tgcagtaaaa gccggactga cacaatttgg ttatgttgtt gactggctaa aagatggcga
      661 agctgctcgg gcggctctca aatcagagtc ttttgaatta attattcttg acttgggatt
      721 acctaaatta tccggtttat cattattgca aaacatcaga catgatggaa atagcacacc
      781 tgtgattatt cttacagctc gagaatcaat agaagatcgg gtaaaaggct tagatagcgg
      841 tgctgacgat tatcttatta agccttttga tttaaacgaa ttaagtgcaa ggattagagc
      901 attggtaagg cgctctcagg gcagagctga ttccgtgttg caatataaaa atattactct
      961 cgatcctgct gctcattctg tcttcgtgga tgatgtactc gtcaatgttc caagaagaga
     1021 atttgctcta ttgcagaaat tacttgaaaa cagtggccag gttttatctc gagaacaatt
     1081 aatgcagagt atttatggct gggaagaaga cgtggacagt aatgctctcg aagttcatat
     1141 acataattta cgtaaaaaac ttaacgcaaa ctttatccgc actatacgtg gtgtaggata
     1201 tatggctgaa aaaaatgaca gtgtacttct atcgtgaaat cgtccattcg aaaattttta
     1261 ttaattaatc tgcttcttgc aattactatt actacaacat taactgctat tggtaactat
     1321 tatcttgatc aaaaagacat tcaagaccac cttgatacat tgatggctat ttccgcgcta
     1381 tcctatcaag ccttattagg ggatgatctt catcaaaggc ctcttgcaaa aattcaaaat
     1441 gccctggaat ctattcccca aaaaattaat acttattatc aaaaaagata tttaaatgat
     1501 gagcctcaag aaaattattt ggataaattt aattttcagg tatggaccaa tggaggtaaa
     1561 ttattattac attcacctac tgcccctaaa ataccattaa cagcagagac agacggcttc
     1621 agcgataaaa gaatttctaa ccaggattgg cgagtattca caacttataa tgacaaagca
     1681 ggaattcgta ctgtactggc agagcgttat gataccagaa atgagctggg tcacagaatt
     1741 gcccaagatg atctctatat tatgttgctt acttttccat tatcaggact attgatatgg
     1801 atcattattg gaagaggact agacagtctg gatagagtag cagaagaggt tgctaaccgc
     1861 gcaccaagtc acctggaacc tgttgattta caagaagttc ccgaagaaat taaacctgta
     1921 attgatgaat taaataaatt attttttcga ctcaaggaag ggtttgagcg tgaaaaacga
     1981 tttgctgccg atgcagcaca tgagttgaga acacctcttg cagcacttaa agcccaagct
     2041 caagtggctt tgaactccaa tgatatagaa gaaaaaaatc aggctttaca aaaattaatt
     2101 gccagtgtta atcgaagtac acatatagtg cagcagttac taaccatgag taaacttgta
     2161 ccagaagcaa cagaactaca tgagcaagat gaagtcaatc taagtaaatt aactcgtgaa
     2221 atattagcta tgttagctcc gtctgcagtt gaaaaacaaa ttgaactgga atttgaaact
     2281 gatgagaaaa tgcctaaaat ttatggaaat ccaaccgctt tgggaatatt aatacgcaat
     2341 ctggttgata attccattcg atattccatg gaaaatggaa gagtcattgt tcgattatta
     2401 aaacataata acgaaatcat actcgaagta tgtgacaatg gcccaggaat cccgcctgaa
     2461 ttacaaaaac gtgtttttga aagatttttt agagtattag gaaacaaaag ccctggtagc
     2521 ggtctgggtt tggctatcgt acagcaaatt tgcgaacttc acggtggaag agtcgtgctt
     2581 gactcaccta aaattggtac aggcttaatt gtgagagttt acttgcccat caaaacaaaa
     2641 ccaaaataaa ctctataatc aaatcataac tctcatcagt taataataac tacaccaccc
     2701 tttcttttgt tcaattaata ccgtacacaa ctttttacca taactcagat tgataacttc
     2761 cccataaaaa ttgtcccttc ttatttttct ttttcggtaa tactaggcaa tgaatctaaa
     2821 atttcctatt ttcagtaata tttcttttga ggtaacttgc tgtaagcctt aatctgtcaa
     2881 aattaacgca tatagtccat aatatacata tatagattat attttaatta aaatgcctca
     2941 aatcctaaat gcattagcgg ctgtcattga agctgacttg gtaaaactaa atcctgagca
     3001 gaaagagatt gttgataaat actctgacaa actcttaaat gttcaatata aagctcatca
     3061 accacgcaca tttgagttag cagtaaaaac catgaaagag caactcgaac aattatctac
     3121 ttcattgaaa aaaataaatt cagagatggc accagacaaa gataaacgtc ggcatgaaat
     3181 gattcaaaaa attgagagtg atttaaaact catagagagt tgtataaacg gggattttga
     3241 aaagaattat caaaatctaa agaaagagtt tttacaattt ttacacgagg ctgatagcaa
     3301 agaccctttt cttaaaaaaa ttcacggtct tt
//
LOCUS       DACTJE010000228         3255 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.230, whole genome shotgun sequence.
ACCESSION   DACTJE010000228 DACTJE010000000
VERSION     DACTJE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.230"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            9..2348
                     /gene="lem26"
                     /locus_tag="I9760_002867"
     CDS             9..2348
                     /gene="lem26"
                     /locus_tag="I9760_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem26"
                     /protein_id="HAT5044605.1"
                     /translation="MKTKLNKSILFKQGNAGELFESFIQKVDENAKPNQLEEIIAVAK
                     PQVIAPVTKNKYTKQFEIIVSALLTNKSNIVFTQNNGKYVVNVNGADFNLDEILANMQ
                     RSQINLSTEQLNQYVNYVDKEAGIKVTIPDAAKKPITLSCQELKGKATKLQHLHEGEI
                     AGLNIYTQQYYEFMNGLLRGQHPYKGNDANFQENMRQVILHSAAALSGISKGQKHKLP
                     LTFRYDGDTLPKPIIEKRIQCAQSKGIGLEVYEDTAFVSSAFEKPVSTFNGPIRTVFY
                     GMQGLDIGEISRYPAEREFLIAPGKVKYLHHREENKKHYFLACSVTPPASIKQISNLP
                     NGGTYKQAENIAVEAERDMVKKFAQFAIDVTNQYLEGKAEKDKSKWSILANIDNTYGK
                     GSYKIQFAEELKTSLNGLLNNLQNGYIEHKAAHNRIIEILHQAIEKAQTIDNISLSYA
                     KGQDSSGVLSKTLQYICFQVEDEMKGLETFKSSITEYFAGHQSFFESSRNIGVIDKLH
                     KDYLSKPYDQSEKGKDPNGGDWTLNCGNNKTLHRPNHGLSHTLRGAALVPEVLYSYAT
                     HSTDKKTRDMVQNISKDDIKRMQVAMLFSVVGRKSELGFADNPGVYGGYRRASADLFE
                     EYAKTHYKDLFTSKEQIAYWKRLVLDYGNPGFHPDVNKPEQAIIAKIMRQCHCLDLLR
                     CYSKEEFTKKVTKPLQDDLGVDAANNLVNYSKTLLEKTGDRLIGTKSYNLQEHWLNNT
                     DVSHCINTLNSALQHAPHQQYVVKHEEEESLDSDMWVKL"
     gene            2540..>3255
                     /locus_tag="I9760_002868"
     CDS             2540..>3255
                     /locus_tag="I9760_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HAT5044606.1"
                     /translation="MNAQSRMKAYTSFLYNCYNDIFEKTPIDFWGYLYFDFQGRYLQL
                     ISEKSIIDEILNKELFIEQNLCKKNYQHDNFYVCNVDGDHVIAPGIKNCLLDRDFTYF
                     VDIIHQDSNRVEMVTFASSQQPNSTNNFIFNNLDYLNMVAENLSSRARRMHTKDNFLT
                     LPRECIIQMNELISSGNSNKKDNLKEIILRSSQQKMAELIKDNVFDYNQLPFSFLAAK
                     ELTHREKEMIYLYFNDFNLSR"
BASE COUNT         1201 a          584 c          559 g          911 t
ORIGIN      
        1 ggtgcatcat gaaaaccaaa ctaaacaaat caatcctttt taaacaagga aatgctggtg
       61 agctttttga gtcttttatt caaaaagttg atgaaaatgc caagcctaat cagcttgaag
      121 aaattatcgc tgttgcaaaa ccccaagtca tagccccagt gacaaaaaat aaatacacta
      181 aacaatttga aattatagta tccgctttac taaccaataa atccaatatt gtctttacac
      241 aaaataatgg caaatatgtt gtaaatgtaa atggggcaga tttcaatctg gatgaaatcc
      301 tggctaacat gcaacgctct caaataaatc tgtctactga acaactaaat cagtatgtta
      361 attacgttga taaagaagca ggaattaaag ttacaattcc agatgctgcg aaaaaaccaa
      421 taactttgag ttgtcaagaa ttgaaaggta aagcaaccaa attacaacat cttcatgaag
      481 gtgaaattgc tggattaaat atatataccc aacaatatta tgaatttatg aatggcttat
      541 taagaggcca gcatccatac aaaggaaacg atgcaaactt tcaggaaaat atgcgtcagg
      601 taatactcca tagcgcagca gcactctcag gaattagtaa aggtcaaaag cataaacttc
      661 cattgacctt ccgatacgac ggagacaccc ttcccaagcc tattattgaa aaaagaattc
      721 aatgcgccca gtcaaaaggt ataggactag aagtttatga agacaccgct tttgtcagta
      781 gtgcttttga aaaacctgta tctactttta atggacccat tcgaaccgta ttttatggta
      841 tgcaaggatt ggatatagga gaaatctctc gttatcctgc tgagcgtgaa tttttaatcg
      901 ctcctggtaa agtcaaatac ctgcaccatc gagaagaaaa taaaaaacac tatttcctgg
      961 cgtgttccgt cactcctcct gcaagcataa aacaaatatc aaatttgcca aacgggggta
     1021 cttataaaca agcggaaaac atagcagtcg aagcagagag ggatatggta aaaaaattcg
     1081 cacaatttgc tatcgatgta acaaatcaat atcttgaagg taaggctgaa aaagacaaaa
     1141 gcaaatggtc aattttagca aatattgata atacctatgg aaaaggctct tataaaattc
     1201 aatttgcaga agaactaaaa acatcattga atggattgct gaataattta caaaatgggt
     1261 atattgaaca taaagccgct cacaacagaa ttattgaaat acttcatcag gccattgaaa
     1321 aagcccaaac tatagataat atttctttat cctacgcaaa agggcaagac agttctggag
     1381 tcctttctaa aaccttacaa tacatctgtt ttcaggttga ggatgaaatg aagggattag
     1441 aaacattcaa aagttcaatc actgaatatt ttgccggaca tcaaagcttt tttgaaagct
     1501 ccaggaatat tggagtcatt gataagttgc ataaagacta tctatcaaaa ccatacgacc
     1561 aatcagaaaa aggcaaagac cccaatggtg gcgattggac tttaaattgt ggtaacaata
     1621 aaactcttca tagaccaaat catgggctgt ctcacacttt aagaggtgct gctctagttc
     1681 cagaggtact ttattcctac gcgacgcatt ccaccgataa gaagaccaga gatatggttc
     1741 aaaacataag caaggatgac atcaaacgaa tgcaagtcgc catgttattt tctgttgtcg
     1801 gcagaaaaag cgaactggga tttgcagata acccaggagt atatggagga tatagaagag
     1861 ccagcgcaga cctttttgaa gagtatgcaa aaacgcatta taaagactta tttacctcaa
     1921 aagagcaaat tgcctattgg aaacgcttgg tacttgatta tggaaaccct ggttttcatc
     1981 cagatgtgaa caaacctgaa caagcaatca tagctaaaat aatgagacaa tgccattgtc
     2041 tggatttatt aagatgctat tctaaagagg aattcacgaa aaaggtaacc aaaccgcttc
     2101 aagatgactt gggagttgac gcagctaata atcttgtcaa ctactccaaa acccttcttg
     2161 aaaaaacagg agacagatta atcggtacga agagctacaa tttacaagaa cattggctca
     2221 acaacactga cgtatcacat tgcatcaata ctctgaactc cgctttacaa cacgcaccac
     2281 accaacaata tgttgttaag catgaggaag aggaatcctt agattcagat atgtgggtta
     2341 aactataaaa taacgtcaaa tagagagaac ctctggtttc aagtaaaact cgggatcaga
     2401 agttctctaa ctttctttga ttactttgtt tatgcattga attgaatcta caaaaataaa
     2461 aaaaatcatt acaccactca acatgattaa tttttgttct ataatttaat caggtagatg
     2521 ttcggattaa agcaacataa tgaatgccca atccagaatg aaagcttata ccagcttcct
     2581 gtataattgt tacaatgata ttttcgaaaa gactcctatt gatttctggg gatacttata
     2641 ttttgatttt caaggacgat acttacaact catttcagaa aaatccatta ttgacgaaat
     2701 tcttaataaa gaattattca ttgaacagaa tctttgcaaa aaaaattatc agcatgataa
     2761 tttttatgtt tgcaatgtag atggtgatca tgtcatagcg cctggtataa agaactgctt
     2821 attagatcgt gacttcacgt attttgttga tatcattcat caggactcca atcgcgttga
     2881 aatggttacc ttcgcctctt cacaacaacc aaacagcaca aataatttta tatttaataa
     2941 tcttgattac cttaatatgg ttgctgaaaa cctgtcttca agagctagga gaatgcatac
     3001 aaaagataat tttttaacct tacccagaga atgcataatc cagatgaatg aactgattag
     3061 ctcaggaaat tcaaataaga aagataatct taaagaaatt attctgagat cctcgcagca
     3121 aaaaatggcg gaactcatca aagataatgt atttgattat aatcaacttc cttttagttt
     3181 tttagcagcc aaagaactga ctcatcgaga aaaggaaatg atctatttat actttaatga
     3241 ttttaattta tcgcg
//
LOCUS       DACTJE010000229         3250 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.231, whole genome shotgun sequence.
ACCESSION   DACTJE010000229 DACTJE010000000
VERSION     DACTJE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.231"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            322..570
                     /locus_tag="I9760_002869"
     CDS             322..570
                     /locus_tag="I9760_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAT5044607.1"
                     /translation="MPKTHVFGALSEFERNLIKERTSAGLEAARARGRQGGQPEKLTS
                     EQKELVKTLYATKKHSIQSICKMVGIGKTTLYKYINQK"
     gene            789..1196
                     /locus_tag="I9760_002870"
     CDS             789..1196
                     /locus_tag="I9760_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044608.1"
                     /translation="MSKSNNINIFVKGKKNQFVFSISSPDFEVLYQSQSKLDVGEDIS
                     PIKADILAIKEGLNFVKENRGNGIIPSTPIISLFTCYENNYHICNGRKNADKFRDELK
                     IINKIKSDLARWQGDHDHVKILWIPAGFKNLMD"
     gene            1418..2500
                     /locus_tag="I9760_002871"
     CDS             1418..2500
                     /locus_tag="I9760_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4917 family protein"
                     /protein_id="HAT5044609.1"
                     /translation="MNITHIMDFSEVLNETQRSPQKKPALLLGNGFSMAFNKSIFSYK
                     SLYEQALEKGLVQKLSSSIPELFESVNTFDFEYIMQVLKYFGIAGAAYSVDENVLMQA
                     KQDEALLKNMLVEAITNNHPDNPNSITLPQYKSCLEFLSNFKSIYSLNYDLLLYWVVM
                     KYLEELGFRDGFCDGNADEEDYYQDEYVVWPTTQSSSAEIYFLHGALHLFDAGHEIRK
                     FCWSRTGIALKEQILQALGQGMFPLFVSEGDAGAKLKKILHNGYLLKGLRSLTQLNGS
                     VVAYGVSFKKNDQHIIDAIIDSTISHLYVSIFGDPTLEPNKDMINMLSTMVEHRNHLI
                     DTKKRKNNLEVHFYNATSAKIWDKHS"
     gene            2658..>3250
                     /locus_tag="I9760_002872"
     CDS             2658..>3250
                     /locus_tag="I9760_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5044610.1"
                     /translation="MKVKRNIIKELRVSRGWTQEHLAHLCDCSVRTIARAEKDGVNSM
                     ETITALAAVFGVEASQLMGMDKIEQLQQKTDEGERYLIRLHSGKMIVESFIESLAYKI
                     DYEIPRNSKDADLISSVIQEIKDWGEIWGDLDVGEQIRASYRLNEIISEIEENGLVLF
                     GLRTREKLNIFSQEIEGFLCNLFISYKDNERIVILGS"
BASE COUNT         1165 a          466 c          613 g         1006 t
ORIGIN      
        1 aaatttttag aaaaatgata gcagaacatg ataaaaagaa atacatttct gtgcatgctt
       61 tttataattt ggataaatcc aaatggcata tattttactt tgattttaaa gatatagaaa
      121 aaattgaaaa tcatcatggc ttgccacacc tgcattatat atcctatctt tggggaaata
      181 attttaagct agatgacgta tggaaaatgt tcaatcaaag aaaagtttct ttaccaaaaa
      241 ttcatataag gtgggaacgt cattaataag tgtaaaaggc ttgtatagga atcctgaaat
      301 aaggtaggca atatttaaaa tatcccgaaa acccatgttt ttggggcact ttcagagttc
      361 gaacgtaact taatcaagga gcgaacctca gctggtctcg aagccgccag agctagagga
      421 cggcagggag ggcagccaga aaagcttaca agcgaacaaa aagagctagt taaaactctt
      481 tatgccacta aaaagcattc tatccaatca atttgtaaga tggttggcat tggaaaaact
      541 accctttata aatatataaa ccaaaagtaa aaaatacaat gatggttaaa ttcgcttagc
      601 attaatttct taaaatgcaa tgaaatttac aatataatta caaaaaactt gactttggtc
      661 gacttttaag ctattattag aatgaaatcc aaggaaattt atggattatc caaccagcag
      721 cctttgtggg tttatattgc agataaggaa aaaacaaccc caataaggct acattattga
      781 ggaacagcat gtccaaatct aacaatataa atatctttgt aaagggtaag aaaaatcaat
      841 ttgttttctc catttcgtct cccgattttg aagttctata tcagagtcag tctaaattag
      901 atgttggtga ggatatatca ccgattaagg cagatattct agcaataaaa gagggattaa
      961 attttgtgaa ggaaaatagg ggaaatggta tcattcctag tacacctata atctcccttt
     1021 ttacttgtta tgaaaataat tatcatattt gcaatggtag aaaaaatgca gataagtttc
     1081 gtgatgagct taagattata aataaaataa agagtgatct tgctagatgg cagggagatc
     1141 acgatcacgt caaaatccta tggatcccag caggatttaa gaacttgatg gattaatatt
     1201 cacgtcatgg gagcatgtct tatcttttgc ctgaatgatt taatcagtga atagttaata
     1261 attttgccag aaatgagtct tataaggcaa gagacaagac gaccccaaac tccaatgcaa
     1321 catctttttt ttaaatttat gcaagaacac tatgtaagct aatagcagtc tagtcttaag
     1381 cccccacgtc aaggggttga tttaatgagg aaccaaaatg aatatcacgc atattatgga
     1441 cttttcagaa gtactcaatg aaacacaaag atcaccacaa aaaaaacctg cccttcttct
     1501 aggtaacggt ttcagcatgg catttaataa aagtattttt tcttataaaa gcctatatga
     1561 gcaggcttta gaaaagggac ttgtgcaaaa attatcctct tcaattcctg aattgtttga
     1621 atcagtaaat accttcgatt ttgaatatat tatgcaagtg ctgaagtatt ttggcattgc
     1681 aggcgctgct tatagtgttg atgaaaatgt cttaatgcag gcaaaacaag atgaagctct
     1741 tttaaaaaac atgttggtag aggcaattac taacaatcat ccagataatc cgaatagtat
     1801 tactttgcct caatataaat cctgtcttga atttctttcc aattttaaat ctatatattc
     1861 gttaaattac gatttattac tatattgggt agttatgaaa tatcttgaag agctaggttt
     1921 tagggatgga ttttgtgatg gaaatgcaga tgaagaagat tattatcaag atgaatatgt
     1981 tgtctggcct actactcaat catctagcgc ggagatttat tttctacatg gtgccttgca
     2041 tttgtttgat gcaggacatg agataagaaa gttttgttgg tctagaactg gcatagcatt
     2101 gaaagagcaa attcttcaag ctcttggaca aggaatgttc cctctatttg tatcagaagg
     2161 cgatgcggga gccaaactaa aaaaaatact tcacaatgga tatttgctta aaggtttaag
     2221 gagtcttacg cagttgaacg gctcagtagt agcttatggt gtaagcttta aaaaaaatga
     2281 tcaacatata attgacgcta taattgatag cacaatttca catctgtatg tcagtatatt
     2341 tggtgatcca acattagaac ccaataaaga tatgataaat atgctctcga ctatggtgga
     2401 gcatagaaat catctgattg atacaaagaa aagaaaaaat aacttagaag ttcattttta
     2461 taatgcaact agtgcaaaaa tttgggataa acattcttga tttaaattag taaacaaaac
     2521 cccccaataa tttactctaa gacatgaact atttttttta tttgcctact gctgtcttat
     2581 tactgtcagt aaactgtcag atagttagga ctattatttt gatatagtta ccttgttgtt
     2641 ataaacagga attcaaaatg aaagttaaaa gaaatattat taaggaactg cgagttagcc
     2701 ggggttggac tcaagaacat ttggcacatt tatgcgattg tagtgtaaga actattgcaa
     2761 gagcagaaaa ggacggagta aatagtatgg aaactattac agctcttgct gcagtttttg
     2821 gagttgaggc tagtcaacta atgggaatgg ataaaataga gcagttgcaa caaaaaactg
     2881 atgaaggaga gaggtattta attaggctgc attctggaaa aatgatcgtt gaatcattta
     2941 ttgaatcatt agcttataaa atcgattatg aaatacccag aaattcaaaa gatgctgatc
     3001 ttatttcaag tgttatacaa gagatcaagg attggggtga aatctggggg gatttagatg
     3061 ttggtgaaca aattagggca tcatatcgat tgaatgaaat tatatcagaa attgaagaaa
     3121 acggcttggt actatttggt ttgagaacta gagagaagct aaatattttt tctcaggaaa
     3181 tagagggttt tctatgtaat ctatttatca gttataaaga taatgagcga atagtaattc
     3241 taggctctga
//
LOCUS       DACTJE010000230         3177 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.232, whole genome shotgun sequence.
ACCESSION   DACTJE010000230 DACTJE010000000
VERSION     DACTJE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.232"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..898
                     /locus_tag="I9760_002873"
     CDS             <1..898
                     /locus_tag="I9760_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044611.1"
                     /translation="KLLTNNHKKIYDFIGTHNQNRIRYFFGNFDNFDPRDNQISVLDR
                     FILDSVHFYITSSLLMQLHKEYQENAMEIDKAWQKEIQAHAHFGPLPTIAIIGNLIKS
                     NKNHVFKDLQFPANKNIQLFQLNKLSIKLNDQVFALWLPKASEAQVSLSNFTRNTIFV
                     CIATAAILAIGVSLFVFSTSSAASLAVIFNPASLGIMIALLACATLMLGISSLYSSTT
                     IHNSTIEHPENRTPEEPYHSHNIRKDKMHSSKAKPEEQLPYHDLKWLLFNRKPYTLRE
                     SIVTPATASEQISSMYTNRQKL"
     gene            complement(971..1642)
                     /gene="mutH"
                     /locus_tag="I9760_002874"
     CDS             complement(971..1642)
                     /gene="mutH"
                     /locus_tag="I9760_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAT5044612.1"
                     /translation="MLALKNKPPKNEKELLEQCQRIEGLSFAQLALELGLFTPVNPVH
                     RKGWVGQALELALGADAKNESLPDFKQLGIELKTIPINKSGKPAESTFIVSIPLLTIH
                     QQSWESSQCYAKLKKVLWIPIEGDTDIPFPHRRIGCGFLWSPNKDQESVLAEDWNYLT
                     NQIVLGQLETLNSFSGEYLQVRPKAANGKSLCYGYDPEGNRIRTLPRGFYLRACFTSV
                     ILSQS"
     gene            complement(1608..>3177)
                     /locus_tag="I9760_002875"
     CDS             complement(1608..>3177)
                     /locus_tag="I9760_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HAT5044613.1"
                     /translation="FILYFTSISYANSQLLIKLIIVWLTFRVLILIARLILLESISDS
                     SGKDVKLYYRLKWLLLFGGWTTALMTFSHLLPLSILLQDIFNRLFMLFMLTVSLVAWK
                     SKDVIPYLIRPLLKSKKRYFKNAISLLVILVPITLFTTAIIGLIGFVNLAWTMSRYQA
                     YILLVLVGYILVRGLIFDALELFSEWMISSLKNGWLWIEVFLKPIDKILRIVLFLTSV
                     LILFQLFGWYSDSLVVTSLEKVVQYSILNITGIHITVASIIEFLILLAVLVWAAKWTR
                     EFCFRWLYKNTKDVGIRHSLSVFTQYAIILLGTFITLHVLGLDFSGMSMILGGLAVGM
                     GFGLRDFASNIVGGIMLLIERPVREGDLITIGEYEGQVAHIGIRSMRVSSWDNTQVLI
                     PNAETFNKPFTNWTHQDGIIRTVIPIKVSRADDPVMIQQLILDVLAIIPEIVPDPPAQ
                     VFLKKIDTALIEFEARYFINIQNHNRVEVRSKVLFAITAQFKAAGIQPPIEPITVELK
                     ESSSVSLKKQTTEE"
BASE COUNT         1100 a          631 c          504 g          942 t
ORIGIN      
        1 taaattgtta actaataatc ataaaaaaat ttacgatttc atagggacac ataatcaaaa
       61 tagaatccga tattttttcg gtaattttga taattttgac ccgcgtgata accaaattag
      121 cgttttagat cgatttattc ttgatagcgt tcatttttat atcactagca gtttattaat
      181 gcagttacac aaagaatatc aagaaaatgc aatggaaatt gataaagcat ggcaaaagga
      241 aattcaagcc catgcccatt tcggtccatt accaacaata gcaatcattg gaaacttgat
      301 taaatcaaat aaaaaccatg tctttaaaga tttacaattc cctgcgaaca aaaatataca
      361 gctctttcaa ttaaataaat tatcaattaa gcttaatgat caagtctttg cgctctggtt
      421 acccaaagca agtgaagcac aagtatcact atccaacttc actcgcaata ccatttttgt
      481 ttgtattgcc accgccgcta ttttagcaat aggcgtaagt ctctttgtat tttctacaag
      541 ctcagcagct agtttagcag ttatttttaa tcctgcatcg ctaggaataa tgattgcatt
      601 gttagcatgt gccacgctaa tgctgggtat aagctctctt tattcttcca ctaccataca
      661 taacagcaca atagaacatc cggagaacag aacaccagaa gaaccttatc actctcataa
      721 tattcgcaag gataaaatgc attctagtaa ggccaaacca gaagagcagt taccctacca
      781 tgatctcaaa tggttattat ttaacagaaa accatataca ttaagagaat caatagtgac
      841 tccagcaaca gcatctgaac aaatatcgtc catgtatact aatagacaaa agctataatg
      901 agctttccct aatatctgta aaatttatca taataccggt aaacagatgg tgcttaattt
      961 atccttaaaa ttaggattga cttaaaatta cggatgtaaa acaggcgcgt aaataaaatc
     1021 ctcgtggtaa cgttcttata cgattaccct ctgggtcata accataacat aaagattttc
     1081 cattggcagc cttaggcctc acttgaagat actctcccga aaaggaattt aatgtttcta
     1141 attgtcccag aactatttga tttgttaaat aattccaatc ttctgcaaga acagattcct
     1201 gatctttatt cggagaccat aaaaatccac atccaattct tctgtgagga aagggaatat
     1261 ctgtatctcc ctctatagga atccataata cttttttgag tttagcgtaa cattgagaag
     1321 actcccaact ttgctgatgg atggtcagta gtggaataga aacaataaat gtggactcgg
     1381 ctggtttccc tgatttatta ataggtatcg tttttaattc aatgcccaac tgcttaaaat
     1441 caggtagcga ctcatttttt gcatcagcac ctaaagctaa ctcgagagct tgcccaaccc
     1501 atccttttcg atgaacaggg tttacagggg taaatagacc caattcaaga gctaactgag
     1561 cgaaactcaa cccctcaatt cgctggcatt gctccaaaag ttctttttca ttcttcggtg
     1621 gtttgttttt taaggctaac actggagctc tcttttagtt caacagtaat aggctcaatt
     1681 ggcggctgta ttcctgccgc tttaaactgt gcagttatgg caaacaagac ttttgacctt
     1741 acttcaaccc ggttatgatt ctggatgtta ataaaatacc gtgcttcaaa ttctattaaa
     1801 gctgtatcga ttttctttaa aaaaacttgt gcaggaggat ctggaacaat ttccggaata
     1861 attgccaaca catcaagtat taactgttga atcatcactg gatcatcggc tctgcttacc
     1921 tttattggta tgaccgtacg aataatacca tcttgatgag tccagtttgt aaaaggttta
     1981 ttgaatgttt cagcattcgg aatcaaaacc tgagtattgt cccatgaaga tacgcgcatc
     2041 gagcgaatgc ctatgtgagc cacttgccct tcgtattcac ctatggtgat caaatcgcct
     2101 tcccttacgg gtctttcaat taaaagcata atgcctccaa caatatttga agcaaaatct
     2161 cttaaaccga aacccatacc gacagcaagt ccaccaagta tcatcgacat accgctaaaa
     2221 tccagtccca gaacatgaag agtgataaag gtacccagta atatgatcgc atactgtgta
     2281 aatacagata aactatggcg tatacccaca tctttggtat tcttatatag ccatcgaaag
     2341 caaaattctc ttgtccactt tgctgcccat actaaaacag caagcagaat aagaaattca
     2401 atgatactgg cgactgtaat atgtatgccc gtaatattaa gaatactgta ttgaactact
     2461 ttttcgaggc tagtcacaac cagtgaatca gaataccaac caaacaactg aaataaaatt
     2521 aatacacttg ttaaaaacaa gacaatacgc aagattttat cgattggttt gaggaaaacc
     2581 tctatccata accaaccatt tttcaatgaa gagatcatcc actcagagaa aagctctaat
     2641 gcgtcaaata tcaaacctct tacgagaata taacctacta aaaccaacag aatataagct
     2701 tgataacgac tcatagtcca tgccaggttc acaaagccaa ttaaaccaat aattgcggtt
     2761 gtaaataagg ttataggaac taaaatgacc aataaagaaa ttgcattttt aaaatatctt
     2821 ttctttgatt tcagtaatgg tctaatgaga taaggaatta catctttact tttccatgcg
     2881 accaaggata ccgttaacat aaaaagcatg aataatcgat taaatatatc ttgcagtaga
     2941 attgataaag gtaacaagtg actaaatgtc atcaacgcgg ttgtccatcc accaaaaagc
     3001 aacaaccatt ttagtcgata atataattta acgtccttac ctgatgaatc cgatatactt
     3061 tctaacaaaa tcaaacgggc tattaaaatt aaaactctaa aggtaagcca cacgataatg
     3121 agtttaatta ataattgaga gttagcataa gaaatactgg tgaagtataa aatgaag
//
LOCUS       DACTJE010000231         3155 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.233, whole genome shotgun sequence.
ACCESSION   DACTJE010000231 DACTJE010000000
VERSION     DACTJE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.233"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            279..1523
                     /locus_tag="I9760_002876"
     CDS             279..1523
                     /locus_tag="I9760_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAT5044614.1"
                     /translation="MKINKTNVENLPLPQAMEAGKTAQKRYYDDNLKGFGIRVTSGGA
                     KAFFVEKFINKKRCRITLGHYPELTAEMAKNKALHLLGQIAMGIDPIAEKKATEVRDI
                     TLNQVFKDYLRTRKNLKPRTIDNYTHILDKAFTHWKSKPMLSITRDRIAKYHEKLGNE
                     HGEAYANLAMRILRALFNFAAGQYEDNSGHSLFIDNPVKRLSQTRAWYRIEKRKTYIK
                     SHELAAWYTSLQEIQNVTLRDYLLLILLTGLRRQEAATLKWSDLDLKAKTFTINTTKN
                     NESHTLPLSDFLYDLLIARKEKAINDYIFPGSGTAGHIIEPRKTMAHVIKNSGVHFTV
                     HDLRRTFITIAESLDIPAYALKRLMNHKMSSDVTAGYIIVDVERLRKPMQLITDYILK
                     CVGVVKSADIIPIQPEKTELLK"
     gene            1520..2551
                     /locus_tag="I9760_002877"
     CDS             1520..2551
                     /locus_tag="I9760_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 domain-containing protein"
                     /protein_id="HAT5044615.1"
                     /translation="MNMPELPELLTAIRSILNQARTQLQQTVNHTMVQAYWNVGRLIV
                     EHEQQGKSRADYGQQQIKLLSEQLCKEFGKGFKERNLRNMRNFYLMYPIWQTVSAKLS
                     WSHYCALLRVEKQSAREWYMKETIENVWSVRALERQIDKLYYERLLSSKDKQPVIDEA
                     NSHIEPLKDDPKNYLRDPYILDFLNLPHASVLESNLEQALINNLQQFLLELGKGFAFV
                     ERQQRIATEDQDFYIDLVFYNFKLKCFLLIDLKIGKLTHQDVGQMDTYVRIYDQHRKS
                     SDDNPTIGLILCSEKSEAVAKYSVLTESKQLFASKYLPYLPTEEELRAELERERAILN
                     ELPDIKQKN"
     gene            complement(2637..>3155)
                     /locus_tag="I9760_002878"
     CDS             complement(2637..>3155)
                     /locus_tag="I9760_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1083 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044616.1"
                     /translation="NYEISSLLTITERKLQSIQMESYLRYENNSISKNLEELSVKITK
                     GEIKPEVEGDKIRVLIDSPVLRRDLEYSITSLGHIVDYSFNKNILSLRLSNFFEVFGN
                     LNIENGKELKTQVIDFFREQNKWDKEILIEIENKSWWIKQFNTLQAAVKKEAAALIFH
                     SIISMVKSHIGI"
BASE COUNT         1073 a          578 c          534 g          970 t
ORIGIN      
        1 attggtacca aaatatcttt ctagaaggga ttcgaattgt aacttattga ttttactatg
       61 ccgttgcgcg gaatcgaacc gcggacctct tgattacgaa ttagtgaatt ataggtttta
      121 gccaattaga caccttttaa aactctttta atatcccttt atttacttga tgttacaaca
      181 ttattacgtt ataagttcta ttaattgatt ttaaataatt gtgcgacaca agtgcgacac
      241 tcttgcgaca cattaaaaag taagggtaaa aattaattat gaaaataaac aaaacaaacg
      301 tagaaaacct ccctcttcct caagcaatgg aagcaggtaa aactgcacag aagagatatt
      361 atgatgacaa cctaaaaggc tttggcatcc gtgtaacttc tggaggagca aaagcctttt
      421 ttgttgaaaa attcataaat aaaaaacgct gtcgcattac tttaggacat tacccagaat
      481 taactgctga aatggcaaaa aacaaagcgc tacacctttt aggtcaaatc gctatgggta
      541 ttgaccccat tgctgagaaa aaggcgacag aggttcgaga tattacttta aatcaagttt
      601 ttaaagacta tctaagaacc agaaaaaact taaagcctag aactatagat aattacacac
      661 acatattaga taaagcattc acccattgga aaagcaaacc gatgttatct atcactagag
      721 atcgcatcgc caaataccac gaaaagctag gtaatgaaca tggagaagcc tatgctaatt
      781 tagctatgcg gatcctacga gccttattca attttgcagc aggccaatat gaagataaca
      841 gcggccattc ccttttcatt gataacccag taaaacgcct ctcgcaaact cgcgcctggt
      901 accgcattga aaaacgaaaa acctatataa aatctcacga gctagctgcc tggtatacta
      961 gtttgcaaga gatccaaaat gtaacattac gtgattacct gttattaatc ttattaactg
     1021 gtttacgcag gcaagaagca gccaccttaa agtggtctga tctcgactta aaggccaaga
     1081 cattcactat caatacaacc aaaaacaatg aatcccatac tcttccgctt tcagactttc
     1141 tttatgattt gttgattgcc agaaaagaga aggccattaa cgactatatc tttcccggct
     1201 cgggtacagc aggccatatt atcgaaccta gaaagacaat ggcacatgtg ataaaaaatt
     1261 ccggtgttca tttcacagtc catgacttga gacgaacatt tatcactatt gccgaaagtc
     1321 tcgatattcc agcctatgct ttaaaacgtt taatgaacca taaaatgagt agtgatgtaa
     1381 ctgccggata catcattgtt gatgtagaac gacttcgaaa acctatgcag cttattacag
     1441 attatatttt aaaatgtgtg ggtgtagtaa aatccgctga catcattcct atccaacccg
     1501 aaaaaacgga gcttttgaaa tgaatatgcc tgaactaccc gaattgctta cagctatacg
     1561 ttcaatacta aatcaagcac gaacccagct acagcaaaca gttaatcata ctatggtaca
     1621 ggcttattgg aatgttgggc gtttaattgt tgagcatgag caacaaggta aaagtagagc
     1681 tgattatggt caacagcaaa taaaattatt atccgaacaa ttatgtaaag agtttggtaa
     1741 gggatttaaa gagcgtaatc ttaggaacat gagaaatttt tatctcatgt atccaatttg
     1801 gcagacagtg tccgccaaat tgagttggtc acattattgc gcattgttac gggtagaaaa
     1861 acagagcgcg cgtgaatggt acatgaaaga aactatagag aatgtttgga gtgtcagggc
     1921 attagagcgt caaattgata aattgtatta cgaacgctta ttatcaagta aagataaaca
     1981 acctgtgatt gatgaagcca attctcatat tgaaccatta aaagacgatc cgaaaaatta
     2041 cttacgtgat ccttatattc tggatttttt gaatttacca catgcttctg tattggaaag
     2101 taatctggag caagcgctta tcaacaatct tcaacaattc ctattagagc ttggtaaagg
     2161 ctttgcattc gtagaacgac aacaacgaat tgcaacagaa gatcaggatt tttatatcga
     2221 tttagtattt tacaatttta aactgaaatg ctttctactt atcgacttaa aaataggcaa
     2281 acttactcat caagatgttg ggcaaatgga cacttatgta aggatttatg atcagcaccg
     2341 taaaagctct gatgataatc ctactatcgg tttgatttta tgtagtgaaa aaagcgaagc
     2401 ggttgctaag tattctgtgt taacagaaag taaacaacta tttgcatcaa aatacttacc
     2461 ctacttacct actgaagaag agttgcgtgc cgaattagaa cgtgaacgcg ctattttaaa
     2521 tgagttacct gatatcaaac agaaaaatta gccaatacct ctattgtcaa ccgggggata
     2581 aacattattt ttgatttcta gtacttaaac attaaaatta ttaccgtaat ttttatttat
     2641 atccctatgt gtgatttcac catagaaatt atactatgaa aaattaatgc agctgcttcc
     2701 tttttgacag cagcttgtaa ggtattaaat tgcttaatcc accatgactt attttcaatc
     2761 tcaattagta tctccttatc ccatttattt tgttcacgaa aaaagtcaat tacttgcgtt
     2821 tttaattcct ttccattttc tatattcaaa ttaccaaaca cttcaaaaaa gttgcttaat
     2881 cttaaactga ggatattctt gttaaaagag taatcaacta tgtggcctaa agaagttatt
     2941 gaatactcta gatctctcct taaaactgga gaatctatta aaactcgaat tttgtcccct
     3001 tcaacttctg gtttaatttc tccctttgta atttttacac ttagttcttc taggtttttt
     3061 gatatggaat tattttcata tctcaggtag gactccattt gaatgctttg caatttcctt
     3121 tctgttatcg tcagaagaga agaaatttca taatt
//
LOCUS       DACTJE010000232         3149 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.234, whole genome shotgun sequence.
ACCESSION   DACTJE010000232 DACTJE010000000
VERSION     DACTJE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.234"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..348
                     /locus_tag="I9760_002879"
     CDS             <1..348
                     /locus_tag="I9760_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAT5044617.1"
                     /translation="QGKIPTNMPEVFDRVFEIIFSNKIRFNTELTEKINEYVETIQEI
                     EKIVPENSFIRQLPGYKRLLNYKYIQDIIYIESSDEENATASFDFSRKAIQRRIDSGY
                     KDAKNNFQLNQLI"
     gene            complement(529..1512)
                     /locus_tag="I9760_002880"
     CDS             complement(529..1512)
                     /locus_tag="I9760_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HAT5044618.1"
                     /translation="MRRLLITILIFLLSNAGCCAPVFEIIPLGVYGGLKDGNLSAYLI
                     KAIESKNYTALDGGTLVRGLEVAVDRQSLPSKKVDDLLQKHIPAYLISHAHLDHVMGL
                     VMAQPELREKQTIMAREETMQALQDNIFNWSVWGNFGDSGAIPHLNFQHYQTLPLLQW
                     LAIPDTDLHVKVFPLSHSGMASSAFLIRYKTEYALYFGDTGADRIEKSTNMENIWKEI
                     APLIRNKQLHAIMLECSFLNSQPDDKLFGHLKPNLFMSELRHLASIVDPFDSRNSLRG
                     LPVVVTHIKPKLADFSNTNEDTEKQVINELSQENDIGVELIKPEQGITLSL"
     gene            complement(1617..2288)
                     /locus_tag="I9760_002881"
     CDS             complement(1617..2288)
                     /locus_tag="I9760_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5044619.1"
                     /translation="MLAPEISLLDHLPGYIGWKDLNRNYLGANKALLELKGFRDIEEL
                     AGKTDEELSPWATEENKMFQQQDLRVLNGEKIATVHFDSKTSEVFLLEKRPLTDQDQK
                     VTGLIYYCHPYPKNEIFRVLRLFDDKWHLSPHYYTLNDNKNIYGLTDREHECVFLLIR
                     GKSAKEIGALLSLSKRTIESYIENIKNKMDCRNKAEILVKAVLNGYHNYFPARLQQAA
                     IIRSL"
     gene            2552..>3149
                     /gene="lem16"
                     /locus_tag="I9760_002882"
     CDS             2552..>3149
                     /gene="lem16"
                     /locus_tag="I9760_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem16"
                     /protein_id="HAT5044620.1"
                     /translation="MFFKREKNTPISFIAILEKYDINPVNLNIASSYDEELHTPAVLT
                     KYLGRDIKFLVAILEANKGHLAKDGELYKNITDALRFAEYDLERAKNNLNSVHSKRKK
                     IKNKNDMNIESEWNAANHALKKCQTHFDRMESFKNWLDETEEEYLDKDTCKLLKSNIK
                     TLQNLADEITHLTKKANLIYQKYKDFWGENQENIEEKLD"
BASE COUNT         1058 a          594 c          543 g          954 t
ORIGIN      
        1 cagggaaaaa taccgactaa tatgcctgaa gtttttgata gagtatttga aatcattttt
       61 tccaataaaa tccgttttaa taccgaatta actgaaaaaa tcaatgagta tgttgaaaca
      121 atacaggaaa ttgaaaaaat agttcccgaa aatagcttta taagacaact cccaggctat
      181 aaacgactgc ttaattacaa atatattcag gatataattt atatagaaag ctcagatgaa
      241 gaaaatgcaa ccgcttcttt tgatttttcc cgcaaagcga tacaaagacg tattgactca
      301 ggatacaaag atgccaaaaa taattttcag ttgaatcagt taatttaaac tctgctgcat
      361 ccactattac attgctgctg tcatagcatg caaagtcgca ggagtagcag cttctctatg
      421 ctaaggtttt ttattctggt tcggactttt cgcaacagcc cctgatacac aaataccata
      481 aatgatttat tagggttggt tctggcggct gttgtgactc ttcatcaatc ataaacttaa
      541 ggttatacct tgttcaggtt taattaattc gacaccaata tcattttcct gggataattc
      601 gtttatgacc tgtttttctg tatcttcatt agtatttgaa aagtcagcta atttgggttt
      661 gatgtgtgta acaacaacgg gtaaacctcg taatgagttt cttgagtcga aaggatcaac
      721 aatcgatgcc aagtgccgca actctgacat aaacaagttt ggttttaaat ggccaaataa
      781 tttgtcatct ggttgtgaat ttaaaaatga gcactccagc atgatggcat gaagctgctt
      841 atttcttatc agtggggcaa tttctttcca aatattctcc atatttgtcg acttttcaat
      901 gcgatcagca ccagtatcac caaaatagag ggcatattcg gttttataac gtattaaaaa
      961 agcgcttgat gccattccgc tatggctcaa aggaaacact ttaacgtgca aatctgtatc
     1021 aggaatagca agccactgga gtaatggcag agtttgataa tgctgaaaat tcaaatgagg
     1081 aattgctccg ctatctccaa aattccccca gacagaccaa ttaaaaatat tgtcctgcaa
     1141 tgcctgcatt gtttcttcac gcgccataat ggtttgcttt tctcgcaatt cgggttgcgc
     1201 catcacaagc cccataacat gatcaagatg ggcatgagaa ataaggtaag ctggaatatg
     1261 tttttgcagc aaatcatcaa cctttttact aggcagggat tgtctatcga ctgcgacttc
     1321 aagcccacgc accaaagtgc ccccatcaag agccgtataa tttttgctct cgatggcttt
     1381 tattaaataa gctgataaat ttccatcttt caaaccacca tagactccaa gaggaataat
     1441 ttcaaagaca ggcgcacaac atcctgcatt agataataaa aaaataagaa tggtaatcaa
     1501 taaacggcgc atgaggattt cctttcaaat gattcaagta agtttgtaaa ataatacagt
     1561 attcagcctt gcttaaatta ccgtatttat acggtagaag ttcagcatca taattattaa
     1621 agtgacctta taatagccgc ttgctgtaat cttgcaggga agtaattgtg ataaccattt
     1681 aacactgctt ttaccagtat ctccgcttta ttcctgcaat ccattttatt cttaatattt
     1741 tctatgtacg actctatagt ccgtttggat agagaaagca aagccccaat ttcttttgcg
     1801 gattttcctc ttattaataa aaacacacat tcatgctcac ggtctgttaa tccatagata
     1861 tttttattgt cattcagtgt ataataatgg ggacttaaat gccatttatc atcaaacagc
     1921 cttaaaactc tgaatatttc attcttggga taaggatggc aataataaat caatcccgtc
     1981 accttctggt cctgatctgt taaagggcgt ttttccaaca ggaaaacttc actagtcttt
     2041 gaatcgaaat gtactgttgc tattttttcg ccgtttaata cgcgcaaatc ttgctgctgg
     2101 aacattttat tctcttcagt agcccaaggc gacaactcct catctgtttt accagccagc
     2161 tcttctatat cacgaaatcc tttcaattcc agcagcgctt tgtttgcacc taaataattg
     2221 cggtttaaat ccttccagcc aatatagcct ggaagatgat ccagcaagga tatttcaggg
     2281 gccaacatta gtcctatcct ccaatgaatg ctaattttgc gcgcggatta ttaaggggtt
     2341 aatgtcgtta ttttaaatgg tagcctgccc aaatcaactg cctgtataac aaaagagagt
     2401 ctaatcaata tttggcttta aaggcaggca ctctcaggca atgaggaaga ccctattatg
     2461 tcgcaaattc gacataagcg ttaattaact ctccagatta attgattaaa ataagaatta
     2521 ttagaaaaaa tattaaccat aggatcttga aatgtttttt aaacgtgaaa aaaatacacc
     2581 tatatctttc atagcaattt tagaaaaata cgacattaac cctgtcaacc tgaatattgc
     2641 ttcttcgtat gatgaagagc ttcatacacc agctgttcta acaaagtacc tagggcggga
     2701 tatcaaattt cttgtagcaa ttttagaagc gaataaaggc cacttggcaa aagacggtga
     2761 attgtataaa aacatcactg acgctctgcg atttgctgaa tatgacctgg aaagagctaa
     2821 aaataatttg aatagcgtac attcaaaacg aaaaaaaatt aaaaacaaga acgatatgaa
     2881 tattgaatca gaatggaatg cggcaaatca tgcgttaaag aaatgtcaga cccattttga
     2941 cagaatggaa tcattcaaaa actggcttga cgaaacagag gaagagtatt tggacaaaga
     3001 cacatgtaaa cttttaaagt caaatataaa aactctccaa aatctggctg atgagatcac
     3061 tcatttaaca aaaaaggcaa acttgatata tcaaaaatat aaagattttt ggggagaaaa
     3121 ccaagaaaac atagaagaaa aattagact
//
LOCUS       DACTJE010000233         3080 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.235, whole genome shotgun sequence.
ACCESSION   DACTJE010000233 DACTJE010000000
VERSION     DACTJE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.235"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..478
                     /locus_tag="I9760_002883"
     CDS             <1..478
                     /locus_tag="I9760_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HAT5044621.1"
                     /translation="TGADGFPAIADAGNVMRPVTSLKLSMRLPPLVDPEAASFAMEKA
                     LTQNPPYNAKVDFKIQNGGSKGWNAPLLSDWLAKAASEASMTYYDKPAAYMGEGGTIP
                     FMSMLGEQFPKAQFMITGVLGPHSNAHGPNEFLHLDMVKKLTSCVSYVLYSFSQKK"
     gene            570..887
                     /locus_tag="I9760_002884"
     CDS             570..887
                     /locus_tag="I9760_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HAT5044622.1"
                     /translation="MKARLIAILLSIIVIPFSSQAENEPQKTPAKQVLVKGKINLNTA
                     DVNSLTGSFKGIGKKRAEAIIAYRENHQGFKSLEELAEVKGIGQRFVDKNREKLKEIF
                     VIN"
     gene            1083..2120
                     /locus_tag="I9760_002885"
     CDS             1083..2120
                     /locus_tag="I9760_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAT5044623.1"
                     /translation="MFRKLRGVFSSDLSIDLGTANTLIYVRDKGIVLNEPSVVALRNE
                     SGQKRVAAVGLEAKRMLGRTPGNINAIRPMKDGVIADFFVTEKMLQHFIHKVHENKFL
                     RPSPRVLVCVPCGSTQVERRAIRESAMGAGAREVFLIEEPMAAALGSGMPVEEASGSM
                     VVDIGGGTTEVAIISLSGIVYHQSVRIGGDKFDDAIVSYVRRNYGTLIGETTAERIKH
                     EIGSAFPSRDLFEIEVRGRNLAEGVPRSFTLTSAEILEALQEPLSGIVGAVRAALELA
                     PPELAADIAERGMVLTGGGALLKNIDTLLMEETGLPVLIAEDPLTCVARGGGKALETM
                     DLRGGDFLSTE"
     gene            2098..3006
                     /gene="mreC"
                     /locus_tag="I9760_002886"
     CDS             2098..3006
                     /gene="mreC"
                     /locus_tag="I9760_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAT5044624.1"
                     /translation="MISSQQNNKHNRLFAKGGHSSLGFVFALAFSILLMFSDYHYRYL
                     DFVRSGFSLIVSPLQYAVDYPVRVIGWIQSLVSAKKALIDENMRLKYKQTMLEAELQK
                     LIVIQRENSQLKELLLTSSKADMRVMAAQILAVDTSQARQVVVLNKGTRDGVYVGQPV
                     LDAKGVMGQVIDVGPMTSTLLLISDSKSAVPVRNNRTGERAILVGTNDIEELSLINLP
                     KTSSIHPGDVLVTSGLGRRYPEGYPVGRVEEVKSIPGEDFVKVTVSPVALLNRNRLVL
                     LIWADSEQEELTAQINERLNAEESTA"
     gene            3003..>3080
                     /locus_tag="I9760_002887"
     CDS             3003..>3080
                     /locus_tag="I9760_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAT5044625.1"
                     /translation="MSVLHIRLGLGFLVALALSILPMPEL"
BASE COUNT          891 a          559 c          729 g          901 t
ORIGIN      
        1 gactggtgca gatgggtttc cagcgatagc tgatgcaggg aacgtaatgc gccctgttac
       61 gtctttgaaa ttatccatgc gtcttccacc actggttgat ccggaagcag cttcttttgc
      121 catggaaaaa gccctgaccc aaaaccctcc ctacaatgca aaagttgatt ttaaaataca
      181 aaatggaggt tccaagggat ggaatgctcc tttgctttct gattggttag cgaaagcggc
      241 atctgaagca tcaatgactt attatgataa acctgctgcc tacatgggag aagggggtac
      301 cattccattt atgagtatgc taggcgagca atttcccaaa gcacaattta tgataactgg
      361 tgttttaggc ccccattcca atgcccatgg cccgaacgag ttcctgcatt tggacatggt
      421 aaaaaaactc acctcatgtg tatcgtacgt tctttatagt ttttcacaga aaaaataatt
      481 tgaaattggc tagactcttg tttgaaaaat tgagatactc gctcgcggct ctggaacgag
      541 ccgtatagtc caacaattag gagaaattta tgaaagcaag actcattgct attttgttat
      601 caattattgt tatccctttc tcttctcaag ctgagaacga gcctcaaaaa actccagcta
      661 aacaagtcct tgttaagggc aagattaatc ttaatacagc agatgttaat tctttaacag
      721 gctcttttaa agggatagga aaaaaacggg ctgaagctat tattgcttat agagagaatc
      781 atcaagggtt taaatctctg gaagagcttg cggaagtcaa gggtataggg cagcgttttg
      841 tagataaaaa tagagaaaaa ctgaaagaaa ttttcgttat taattaattc acatgcaata
      901 taaaatgtaa atcatgtttt ataaagcaaa tgagattgat cccatatgaa agcattgctt
      961 ttactatggg atccggttaa aagcagttgt ctggtgtata aaattcttat gcagcaactg
     1021 tcgattttta tccaaatata ggataaagta gcaatcaaaa aaacaacggg gtagtactgc
     1081 gtatgttcag gaaattaaga ggcgtgtttt ctagcgattt gtcaatcgat ttgggaacag
     1141 ctaatacatt gatttacgta agagataagg ggattgttct caatgaacct tctgtagtgg
     1201 cattgcgtaa tgaatcaggc cagaagcgtg tcgccgctgt tggtcttgaa gccaaacgca
     1261 tgttgggaag aacccctgga aatattaatg cgataagacc catgaaagat ggggttattg
     1321 ctgacttttt tgtcaccgag aaaatgctgc agcactttat acacaaagtg catgaaaata
     1381 aatttttacg acccagtcca cgagttttgg tctgtgttcc ttgtggttct acacaagtgg
     1441 aacgaagagc aattcgtgaa tctgcaatgg gtgctggtgc tcgagaagtt ttccttattg
     1501 aagagcctat ggctgcggca ttaggttccg gaatgcctgt tgaggaagcc agtggttcta
     1561 tggtggttga tataggcggt ggtaccacag aagtcgcgat tatttcacta agtggaatcg
     1621 tttatcatca atcagtacgt attggtggcg ataaatttga tgacgcaatc gtatcctacg
     1681 tacgccgtaa ttatggtact ctcatcggag aaacaactgc tgaacgcatt aagcatgaaa
     1741 ttggttcggc tttcccaagc cgggatttat ttgagatcga ggtacggggt agaaatcttg
     1801 cagaaggtgt accacgtagc tttactttaa caagcgctga aatcctggaa gcactacagg
     1861 aacctctatc agggatagtt ggtgcggttc gagctgcttt ggagcttgct ccgcctgagt
     1921 tggctgcaga tattgctgaa agaggtatgg ttttaacagg cggaggcgct ctgttgaaaa
     1981 atatagacac cttgttgatg gaagaaactg gattacccgt attaatagct gaagatccat
     2041 taacctgcgt tgcgcgtggt ggtggtaaag ctttagaaac catggatttg cgcggcggtg
     2101 atttcctctc aacagaataa taaacacaat agattatttg ccaagggcgg acacagttct
     2161 ctgggatttg tgtttgccct tgctttttct attctcctga tgttttctga ttatcattat
     2221 cgatatctgg atttcgttcg tagcggtttt tcactaatag tttcgccctt gcaatatgcg
     2281 gtagattatc ctgttcgagt catcggttgg attcagtcgt tagttagtgc caaaaaggcg
     2341 ctgatagatg aaaatatgcg tttaaaatac aagcaaacaa tgcttgaagc tgaattgcaa
     2401 aaattaattg ttatccagag agaaaattca caattaaaag aattattatt gacttcatcc
     2461 aaagctgata tgagagtcat ggctgcccag atactcgctg ttgatacaag ccaggcaaga
     2521 caagttgtcg tcttaaacaa aggcacacgt gatggtgttt atgtgggaca gcccgtgttg
     2581 gatgccaagg gagtgatggg gcaggttatt gatgtaggac caatgacaag cactttatta
     2641 ctgatttcag attcaaaaag tgctgtgcca gtaagaaaca atcgaaccgg agagcgcgca
     2701 atccttgtag gcactaatga tattgaagaa ctgtctttga ttaatttacc gaagacttct
     2761 tctattcatc caggtgatgt gctggtaacg tccggtttag gccgaagata tccagagggc
     2821 tatcctgtag gacgcgttga ggaagttaag agtattcctg gcgaagactt tgtcaaagtc
     2881 acagttagcc ctgtcgcatt gctgaatcgc aacaggttgg tgttattgat ttgggctgat
     2941 agtgaacaag aagagttaac tgctcaaatt aatgagcgat tgaatgctga ggagtctaca
     3001 gcatgagcgt gcttcacatt cgtttaggat tggggtttct cgtggcactt gctttatcaa
     3061 tactaccgat gcctgagtta
//
LOCUS       DACTJE010000234         3044 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.236, whole genome shotgun sequence.
ACCESSION   DACTJE010000234 DACTJE010000000
VERSION     DACTJE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.236"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..806
                     /locus_tag="I9760_002888"
     CDS             <1..806
                     /locus_tag="I9760_002888"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAT5044626.1"
                     /translation="NALEINYRLMEACSKTKWAMGVGSQRRELTDKQAAFEWTPLRRD
                     FPMVSLFSNLGIAQLIDTPISAIQRLIDTLQAEALIIHCNPLQECIQPEGTTNFQGCW
                     TALEALVKKIASPVIIKETGCGFSKNTLLRLNNIGVAAVEISGVGGTHWGRIEGHRAN
                     KDPIRQRTADTFRNWGIDTLQSTRNAISLNPSFEIWGSGGVRNGLDAAKLFALGATTV
                     GFAKPMLEAALGSTGQVLTQMNTIEYELKTAMFCTGSRVLDDLKEKACP"
     gene            797..2095
                     /locus_tag="I9760_002889"
     CDS             797..2095
                     /locus_tag="I9760_002889"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAT5044627.1"
                     /translation="MSIAANAGELFRGFSKLSREERFQRLCALGALTHEDITFLKQGG
                     IKDLNLADKLIENVIGYFQLPLGVATNFNIDGRDYVIPLAVEETSIIAALSKSAKWIR
                     QHGEINTWVHGECILGQIQLAKIKDFQRFSDLFNKNRQYFIEIANKDVAANMVKRGGG
                     VTDLQVRHLKRDDGQDMAVIHLTMNSCDAMGANIINQVLEYLKQPIEQITGEEVTMCI
                     LSNLNDQKLTTAQVIIRNIDPILGQKLQEASLFAEIDPYRAATHNKGVMNGIDPVLIA
                     TGNDWRAVEAGIHAYAARSGQYKAITRWRYQNEILTGELTAPIIVGTVGGVTSLHPTA
                     KMCLRMMDITSANQLSQVIAAVGLVQNLGALKALCTDGIIQGHMKLHIDNLLLVAGAN
                     ENEMPVLKEKLQEWLNLNKRVSLNNAYDLLAEIRQAPVAV"
     gene            2092..2970
                     /locus_tag="I9760_002890"
     CDS             2092..2970
                     /locus_tag="I9760_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044628.1"
                     /translation="MKWQIPAKTFLLGEYAAIAGEPAILLTTSPCFTLTLTENAKLEG
                     IHPESPAGLWWQKNQCHPFGLSWQDPYHGQGGLGASSAQFLACYLATCSLQEITPQLN
                     HMLEAYYQSSWSGLGLRPSGYDVIAQSQQGCVYIDKQKNQVNSFSWPFKDLSFLIIHT
                     GNKLATHHHLQEVTLPSQIDYLASLVIKAKEAFDLARSHHLIQCINLYAQKLTELNLV
                     SQHSLPLIQSIKSYPETLAIKGCGALGADTLLILTDKRDLQSLKDKVQDQNWLILATE
                     DNLTVRNNNSLFEKSI"
BASE COUNT          949 a          606 c          635 g          854 t
ORIGIN      
        1 ccaatgctct agaaattaac tatcgattaa tggaagcgtg ctcaaaaaca aaatgggcaa
       61 tgggcgttgg ctcacaacgc agagaattaa ccgataaaca agccgcattt gagtggacgc
      121 cactccgtcg tgatttcccc atggtcagcc tgttcagtaa tctgggaatc gctcagttaa
      181 ttgacactcc aatttctgca atccagcgct taattgatac attgcaagct gaagcattga
      241 ttattcattg caatccatta caagagtgta ttcaacctga agggacaact aattttcaag
      301 ggtgctggac agcattggaa gcgttagtca aaaagattgc ttctccagtt ataataaaag
      361 aaactggttg tggtttttca aaaaatacgc tgctccggtt aaataatata ggtgttgccg
      421 ctgttgaaat cagtggtgtg gggggtacgc attgggggcg aattgaagga catcgggcaa
      481 acaaggatcc gataagacaa cgaactgctg atacttttcg aaattggggc attgatactt
      541 tacaaagcac tcgtaacgcc attagcctta atccctcttt cgaaatttgg ggttctggcg
      601 gcgtaagaaa tggtttggat gctgcaaaac tgtttgcgct cggtgctacc acagtcggct
      661 tcgcgaaacc catgcttgaa gcggctttgg gctcaaccgg gcaagtactg acccaaatga
      721 atacaattga atatgaattg aaaactgcta tgttttgtac tggcagccga gttcttgatg
      781 atttaaagga gaaggcatgt ccatagctgc taatgccggt gaattatttc gcggattctc
      841 aaagttatca cgtgaagagc gctttcaaag gttatgcgct ttaggtgcct tgactcatga
      901 agacataaca tttcttaaac aaggtggtat taaagattta aacttggccg acaaattgat
      961 tgaaaatgtg attggatatt ttcagcttcc attgggtgtg gctactaatt ttaatattga
     1021 tggacgtgat tatgtaatac ctttggcagt ggaagaaacc tctattattg ctgctctttc
     1081 caaatcagcg aaatggatta ggcagcatgg tgaaatcaat acctgggttc acggagaatg
     1141 tattcttggg caaattcaac tggcaaaaat aaaggacttt caaagattct ctgacctctt
     1201 taataaaaat cgtcaatatt ttatagaaat agcgaataaa gacgttgccg ccaatatggt
     1261 caaacgaggc ggcggtgtta cggatttgca agtacgtcat ttaaagagag acgatggaca
     1321 agatatggca gtaattcatt tgacgatgaa cagctgcgat gctatgggtg ccaatatcat
     1381 caatcaagta cttgaatatt taaaacaacc catagaacaa ataactggtg aagaagtcac
     1441 catgtgtatt ttatcgaact taaatgatca aaaattaacg accgctcaag tcatcattcg
     1501 taatattgac cccatcctcg gtcaaaaatt gcaagaggcc tcactctttg cagaaataga
     1561 tccctatcgc gctgctaccc ataataaagg ggttatgaat ggaattgatc cggttttaat
     1621 tgctacaggg aatgattggc gagctgtgga agctggaata cacgcctatg cggcacgttc
     1681 gggacaatac aaagcaatta ctcgctggcg gtatcaaaat gaaatactca caggagagtt
     1741 aactgctcct atcatagtag gtactgttgg aggcgtaacc tctttacacc ccacggctaa
     1801 aatgtgttta cgaatgatgg atatcacttc tgccaatcaa ctctcccaag tcatcgcagc
     1861 agttggacta gtgcaaaatc ttggcgcact caaagcttta tgcactgacg ggatcattca
     1921 aggccatatg aaattgcata ttgataattt gctattagtt gcaggggcta atgaaaatga
     1981 aatgcccgtg ctcaaagaaa aattgcaaga atggcttaac ctgaacaaac gagtcagctt
     2041 gaataacgct tatgacctat tagctgaaat ccgacaagct cctgttgcag tatgaaatgg
     2101 caaattcccg ctaaaacatt tttactagga gaatacgctg ccattgcggg agaaccagct
     2161 atcctattaa cgacaagccc ttgctttact cttacattaa ctgaaaatgc aaaattagaa
     2221 ggaattcatc cggagtcacc tgcaggatta tggtggcaaa aaaatcaatg ccatcccttt
     2281 ggcctatcct ggcaagaccc ttatcatggc caaggcggcc tgggcgcatc cagcgcgcaa
     2341 tttttagcct gttatttggc aacttgttca ctgcaagaga ttacccctca attaaaccat
     2401 atgcttgagg cctattacca atcgtcctgg tcggggttgg ggttaagacc cagtggttat
     2461 gatgtgattg ctcagtcaca gcaaggttgt gtttacattg ataaacaaaa aaatcaggtt
     2521 aattcgttct cctggccatt caaagattta tccttcttga tcattcacac cggcaacaaa
     2581 ttggcgacac atcatcatct tcaagaagtc accctaccat ctcaaataga ttacctcgcc
     2641 tctttggtta tcaaagcaaa agaagctttt gatttggctc gtagccacca tttaatccaa
     2701 tgtattaact tatatgctca aaaattgacg gaattaaatc tggtgagtca acatagtctg
     2761 cctcttattc agtctataaa atcttatccg gaaaccttgg caataaaagg ctgtggtgct
     2821 ttaggagctg atactttgtt aattcttacc gataaaagag acttacaatc tctcaaagat
     2881 aaagttcaag atcaaaactg gttaatttta gctacagaag ataatctaac tgtcaggaat
     2941 aataattcct tgtttgaaaa aagcatataa ataacacatc ttgtttgtta aacaaaaaat
     3001 aatctctaaa aagacttgaa attctgctct aatccggtat gatt
//
LOCUS       DACTJE010000235         3009 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.237, whole genome shotgun sequence.
ACCESSION   DACTJE010000235 DACTJE010000000
VERSION     DACTJE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.237"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            208..1341
                     /locus_tag="I9760_002891"
     CDS             208..1341
                     /locus_tag="I9760_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT5044629.1"
                     /translation="MTSSKHYKESLALLFEGSLRAIPFNIILAILLALDLVYSDVPAL
                     IIGAWFLGIVFISSVRSLFCWKIIKRGVEKNRIQATLIEFSLLTFVTGGIWGACYFIT
                     LPYLNDLHEFIIILVLGGMCAGAIASLSIYLPVYYAYVFPMLLPVIIYNYALLDVDRT
                     ILATMFLVFIAVLIISAKINHRLLTENFRLFNEKELLINELKVMSITDSLSGLYNRRY
                     FDNIFPQEFNRARRNQYFLSLVSIDIDNFKLINDNLGHPFGDKVLISIADLLKKIFRR
                     SNDILFRLGGDEFAIIIANQPIKETISLCQIIKNPFKTENLNHTYDSNEKLLMSHVTL
                     SIGIAYIHFESSVSIERAITAADKALYQAKRKGKNQIVVKKLL"
     gene            complement(1404..2045)
                     /locus_tag="I9760_002892"
     CDS             complement(1404..2045)
                     /locus_tag="I9760_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044630.1"
                     /translation="MKMKLQLIAIGILLSINVFADGYYPEPTSQDQPWTIIASFGSGR
                     YQHMYSKDGKTALGRLALANELMLSGDIALGLELGVQNGNKMRLNIPRETLSVLGWLP
                     VETNLSPMLDLLVTAKSDPLFGSSFFAQLKGGVAYRSWQVDLYPINEISQLAGEIQAG
                     FGYPITALASLSLLYQGVFGNDPNVKLNVSSKTGSVSNIPVLHGILLGFTVNL"
     gene            complement(2218..2305)
                     /locus_tag="I9760_002893"
     tRNA            complement(2218..2305)
                     /locus_tag="I9760_002893"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2269..2271),aa:Ser,seq:gga)
     gene            2529..2963
                     /locus_tag="I9760_002894"
     CDS             2529..2963
                     /locus_tag="I9760_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044631.1"
                     /translation="MTLQHSIQKGVSDFSFIIGTGLKYGGAGGLLVMAVVGTVAIDLV
                     LLAAAEKHHNSFLTGFVLGSMFSRGNLDPVPLLIASPITSAVAVVLSVALGVPGVGLA
                     ILAGWTLAGTLLAVGMGLEAFAKSINPEPSSESDYNSCLCFN"
BASE COUNT          894 a          521 c          598 g          996 t
ORIGIN      
        1 cttgggtttt tcgtaagccc gcaaactaaa aggcttaata gattaaatta tagtctctaa
       61 tacattaata aggataaaaa atttgaatag ctttatgcaa aaaagaagat aaaagaatct
      121 atatgtttta aaaatgtcag tgttctataa ttaaccagtg ttctataatt aaaaggggtg
      181 aatattaatt aatggacatt cgataagatg acttcctcca agcattacaa ggaaagtttg
      241 gctttgctat ttgagggatc tctaagagcg attccgttca atattatatt ggccatattg
      301 cttgctcttg accttgttta tagtgatgtg cctgcattaa taatcggggc atggtttttg
      361 ggtattgtct tcataagctc ggtaagaagt cttttctgct ggaaaataat taagcgggga
      421 gtagaaaaaa atagaataca agccacattg atcgagtttt cccttcttac atttgtcacg
      481 ggtggtattt ggggtgcgtg ttacttcatt acactgcctt atttaaatga tctgcatgaa
      541 tttattatta ttttggtact tggcggaatg tgcgcaggcg cgattgcttc tctctctatt
      601 tatttgccag tttattatgc ttatgttttt ccaatgcttt tgccagtcat tatttataat
      661 tatgccttgt tagatgtcga tagaacgatt ttagcaacca tgtttctagt atttattgcc
      721 gtgcttatta tttctgcaaa gattaatcat agattactca ctgaaaattt tcgattgttc
      781 aatgaaaaag aactattaat caatgaattg aaagtgatgt caatcactga ttctttgtcg
      841 gggttatata acaggcgata ttttgacaac atatttccac aagagtttaa ccgagcaaga
      901 cgcaatcaat attttttaag cctggtttca atagatatag ataattttaa attaatcaat
      961 gataatttgg ggcatccttt tggcgataaa gtcctgatta gcatcgctga tttattgaaa
     1021 aaaatatttc gacgttcaaa cgatatattg tttcggcttg gcggagacga atttgcaata
     1081 atcatcgcta accagccgat taaagaaaca atatctcttt gccaaataat taaaaatcct
     1141 tttaaaacag aaaatttgaa ccatacttat gattcaaacg agaaattact catgagtcat
     1201 gtcactttaa gcataggaat tgcttatatt cattttgaaa gttcagtcag tatagagcgt
     1261 gcaattactg cagcggataa ggcattatat caagctaaaa gaaaaggaaa aaaccaaatt
     1321 gtagtaaaaa aactacttta agaagatgat attctatctt taacagagat ggcagcctgg
     1381 aagctgcgtc gtaccttaat ggattataag ttgacagtaa atcctaataa aattccatgt
     1441 aatacaggga tatttgaaac actgccggtt tttgagctca cgtttaattt gacgttagga
     1501 tcgttaccaa aaacgccctg atacagcaag ctcaaactgg ccaaagcggt gataggataa
     1561 ccaaaaccag cttgaatttc tccggctaat tgtgaaattt cattgatagg gtagagatca
     1621 acttgccaag acctgtaagc gacaccacct tttaattgtg caaaaaaaga acttccaaat
     1681 aaggggtcgc ttttggcggt aactaataag tccagcattg gactgaggtt ggtttctacc
     1741 ggtagccatc caaggactga taaggtttct cgcggaatat tcaatcgcat tttattgcca
     1801 ttttggaccc caagctctaa tcccagagca atatctccac tcagcattaa ttcattagcc
     1861 agagccaatc ggcctaaagc ggttttgcca tcttttgagt acatgtgctg gtatctgcca
     1921 cttccaaaac tggcaataat tgtccatggt tggtcttgag aggtgggctc aggatagtaa
     1981 ccatcagcaa atacgttgat tgataataaa attcctattg caattagttg taatttcatt
     2041 ttcatctttc atatccctga ttgaattggt tggttctgaa agcagtttta ccaaaagaat
     2101 cttatatgag ccagctgtag tctttacaca atggatataa tttactgctt tatgcaactc
     2161 aatgaaagcg gcaattatag ccttataccc tgtgaaggat aaccaggaat tgtaatttgg
     2221 cggagagaga gggattcgaa ccctcgatac gttgccgtat acacactttc caggcgtgcg
     2281 ccttcgaccg ctcggccatc tctccaaccg ggcaagctta tacttgtcgg caaataaaag
     2341 caagactgtg ataactatta aaagaggttt tccatcacaa agataatata ttttattttt
     2401 gtatgggaat aaattgtatc gctttatttg agttttaatc caatgtactc gttacttcag
     2461 tgttcaatta gtgttgatta tgtgcaaaat caagtgtatt atttttcaat tttttaggag
     2521 taaggttaat gacactgcaa cattccatac aaaaaggggt atctgatttt agttttatta
     2581 ttggcacagg tctaaagtat ggtggtgctg gcggtctttt agtgatggct gttgtaggaa
     2641 cggttgctat tgatcttgtc ttgcttgctg ctgcagaaaa acatcataac agttttttga
     2701 caggatttgt gttaggctca atgttttcca gaggtaactt ggatccggtt ccacttttga
     2761 tcgcgtcccc catcacatca gctgttgctg tcgtgttatc tgttgccttg ggcgttcccg
     2821 gggtcggtct tgcgatactg gctggatgga ctttggctgg cactttattg gctgttggta
     2881 tgggcttgga agcgtttgcc aaatccatta atcctgaacc ttcatctgag tcagattaca
     2941 attcttgttt gtgctttaac taatagagcc aagaggggag agcaggttat ttcccctctt
     3001 aaaacagtt
//
LOCUS       DACTJE010000236         2951 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.238, whole genome shotgun sequence.
ACCESSION   DACTJE010000236 DACTJE010000000
VERSION     DACTJE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.238"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..662)
                     /locus_tag="I9760_002895"
     CDS             complement(<1..662)
                     /locus_tag="I9760_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAT5044632.1"
                     /translation="MKMKLVTAAVMGLAMSTAMAATDATSLATDKDKLSYSIGADLGK
                     NFKNQGIDVNPEAMAKGMQDAMSGAQLALTEQQMKDVLNKFQKDLMAKRTAEFNKKAD
                     ENKVKGEAFLTENKNKPGVVVLPSGLQYKVINAGNGVKPGKSDTVTVEYTGRLIDGTV
                     FDSTEKTGKPATFQVSQVIPGWTEALQLMPAGSTWEIYVPSGLAYGPRSVGGPIGPNE
                     TL"
     gene            867..2243
                     /locus_tag="I9760_002896"
     CDS             867..2243
                     /locus_tag="I9760_002896"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAT5044633.1"
                     /translation="MNISVFDLFSIGIGPSSSHTVGPMLAANAFLQLLEQKNLFDKTQ
                     RVKVELYGSLALTGKGHGTDKAILNGLENKAPETVDPASMIPRMHEILDSNLLNLAGK
                     KEIPFHEATDFLFLQKELLPKHSNGMRFSAFDGNANLLIEQVYYSIGGGFITTEEDFD
                     KSTTDTNPPPYPFATATELLKLCKKHHLTIAELMLVNEKTWRSTAEIHKGILDIAKVM
                     DDCINNGCKHDGVLPGGLNLKRRAPDLYRKLIEQKGVKSVFEQSDIMNHLNLYAMAVN
                     EENAAGGRIVTAPTNGAAGIIPAVLKYCQQAHDRMSNEDIYTYFLTAAAIGILYKKGA
                     SISGAEVGCQGEVGVASSMAAAGLTAVLGGTIEQVENAAEIAMEHHLGMTCDPVLGLV
                     QIPCIERNAMGAVKAVNATRMALIGDGQHQISLDKVIKTMKQTGMDMQSIYKETSMGG
                     LAVNLPEC"
     gene            complement(2342..>2951)
                     /locus_tag="I9760_002897"
     CDS             complement(2342..>2951)
                     /locus_tag="I9760_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HAT5044634.1"
                     /translation="FWQRWHITLSLWIRDYLYIPLGGNRGRLYQTVNNLLITMFIAGL
                     WHGASWNYAIWGIYHGCLLAVYHVFRFYYIKGNLQTQNQLMKIGSIIMTYSLVVIGWV
                     IFRSTSFEQAFTILGKMYDIPGFVQEFAMNQRASAYTDSLFYLVGMFALCFIGPTFKS
                     IETKILSAVQPFWIKKWLYSLSIFTVLIFMFSPVKPFIYFVF"
BASE COUNT          951 a          610 c          562 g          828 t
ORIGIN      
        1 attaaagttt catttgggcc aataggtccg ccaacgctac gtgggccata tgcaagacct
       61 gagggaacat aaatttccca agttgatcca gctggcatca attgcaaagc ttctgtccat
      121 cctgggataa cttgtgaaac ctgaaaagtt gctggcttac cagttttttc ggtactgtca
      181 aaaacggtac catcaatcag acgaccagtg tattcgacag tgactgtatc cgatttaccg
      241 ggtttaacac catttccagc attgattact ttgtattgca aaccacttgg caatacaaca
      301 acgcctggct tgtttttgtt ttcagttaaa aaggcttccc cttttacttt attttcatcc
      361 gctttcttat tgaattcagc agtacgcttt gccatcaaat ctttctgaaa cttgttaaga
      421 acgtctttca tttgctgttc ggttaaagcc aattgagcgc cactcatagc gtcttgcatg
      481 cctttagcca ttgcttccgg attaacatct atgccttgat ttttaaaatt cttccccaaa
      541 tcggcaccaa tgctataaga caacttatcc ttgtctgtag ctaatgatgt ggcatcggtt
      601 gcagccattg ctgttgacat tgcaagcccc ataacagccg cagtcaccaa tttcatcttc
      661 ataaacaatc cccttttagt cttacactta agttctcata ctgcacaata aattgagcaa
      721 aataaagcaa aaacaaatcg cccactaatt ttgcctaaat ttgcgtaaaa ttaccagtca
      781 taagctatca tttaattata taatggacaa aaaggtaata tccttctaca cttgccccta
      841 ataatgaaca agagatttta acggcgatga acattagtgt ttttgattta ttttctatag
      901 gaattgggcc atcaagctca cacacagtag gccctatgtt agctgccaat gcttttttgc
      961 aattactgga acaaaaaaat ctgtttgata aaactcagag agtcaaagta gagctttatg
     1021 gttcgctggc gcttactggc aaggggcacg gtactgataa agccatttta aatggtcttg
     1081 aaaacaaagc tcccgagaca gttgatcctg cctccatgat cccaagaatg catgaaattc
     1141 ttgattctaa tctactcaat ttggccggca agaaagaaat tccttttcat gaagcgaccg
     1201 attttttatt tttacaaaaa gaattattac ccaaacacag taatggaatg cgcttttctg
     1261 cttttgatgg aaacgctaat ctactgattg agcaagtcta ttactctatt ggcggcggtt
     1321 ttattacaac cgaagaagac tttgataaat caacaaccga taccaatcca cctccttacc
     1381 cctttgcaac agcaactgag ttattgaaat tatgcaaaaa acatcacttg accatcgctg
     1441 aattgatgtt agtcaatgaa aaaacatgga gaagtacagc agaaattcat aaaggaattc
     1501 ttgatattgc caaagtaatg gatgactgca tcaacaacgg ttgcaaacat gatggagtgc
     1561 ttcccggtgg ccttaattta aagcgacggg cacctgactt gtatagaaaa ctaatagaac
     1621 aaaaaggggt taaaagtgtt tttgaacagt cagatattat gaatcatttg aacctttatg
     1681 ctatggctgt caatgaagaa aacgcagcgg gaggacgaat tgtcacagct ccaacaaatg
     1741 gagctgccgg tattattcca gctgttctta aatattgtca gcaagctcac gacaggatga
     1801 gcaacgaaga catctacact tattttctga ctgccgcagc gattggaatc ctttataaaa
     1861 aaggcgcttc aatttctggc gctgaagtag gatgccaagg tgaggttggt gttgcctctt
     1921 ccatggcagc tgctggtttg acagcggttc ttggcggaac catagagcag gttgaaaatg
     1981 ctgcagaaat tgcaatggaa catcatctgg gaatgacctg tgatcccgtt ctgggccttg
     2041 tgcaaatccc atgcatcgag agaaatgcca tgggggctgt gaaagcagtt aatgcaaccc
     2101 gaatggcact cataggtgat ggtcagcatc aaatctcttt ggataaagtc atcaaaacaa
     2161 tgaagcaaac cggcatggat atgcaaagta tatacaagga aacgtccatg ggtggattgg
     2221 ctgttaactt gcctgagtgt tagttatcac cgctccctca cggtcgcggc tcggatgatt
     2281 aaatcaaatg ataattaaac ctgagccgcg accgtgaggg agcggataac aaattatcta
     2341 attaaaaaac aaaataaata aacggtttca caggggaaaa cataaaaatt aacaccgtaa
     2401 aaattgaaag cgaataaagc cattttttaa tccagaatgg ctgtacagct gacaggattt
     2461 tcgtttcaat tgatttaaaa gttggcccta taaaacacag ggcgaacatt cccacgagat
     2521 aaaacaatga atcggtataa gctgaagcac gttgattcat tgcaaattcc tgcacgaaac
     2581 caggaatatc atacatctta ccgagaatag taaatgcctg ttcaaatgaa gtagatcgaa
     2641 atattaccca gcctatgact actaatgaat aagtcattat aattgagcca atcttcatca
     2701 attgattttg agtttgtaaa tttcctttaa tgtaataaaa ccggaaaaca tgataaacgg
     2761 ctaacaaaca accatggtat atgccccaaa tcgcataatt ccaggaagcg ccatgccata
     2821 aaccagcaat aaacatagta attagtaaat tattcaccgt ttgatacaaa cggcctcgat
     2881 tacctcctag aggaatatat aaataatctc taatccataa tgacaaagtg atatgccaac
     2941 gctgccagaa t
//
LOCUS       DACTJE010000237         2927 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.239, whole genome shotgun sequence.
ACCESSION   DACTJE010000237 DACTJE010000000
VERSION     DACTJE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.239"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..605)
                     /locus_tag="I9760_002898"
     CDS             complement(<1..605)
                     /locus_tag="I9760_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAT5044635.1"
                     /translation="MRYFSRLVIFMMMYFVVCEVLAATPFTFKQALDTAYRNNPELQA
                     EMDKARAMRGYFIQSGLYPNPQLTLTAENFGGSGAYSSYESAETTFSVTQPIPLGHRL
                     QYLKKATYADYLASLASIRVQQTALYMAVGVAYVDALYAGQWHQVTKKLTRLNQDIVA
                     AIERRVKAGAGAELDLRLAQVRLGEAKIQENKAARDALLQRA"
     gene            989..1498
                     /locus_tag="I9760_002899"
     CDS             989..1498
                     /locus_tag="I9760_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAT5044636.1"
                     /translation="MPGKIDLAIAAITITDERQQAFLFSLPYLASEARFVTTKNNSLK
                     SLKKLTHKKTGIIRDSLFKQLITQKFKSNSIKEYPSTDKLITALNQYEVDVLLMDDFS
                     ADYWIANNNRLFKGVGDTIPIGIGYGIMAKKNSMALINKMNQALKQIERNGIYLSLYN
                     KYFHTIPMH"
     gene            1807..2418
                     /locus_tag="I9760_002900"
     CDS             1807..2418
                     /locus_tag="I9760_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HAT5044637.1"
                     /translation="MERLLIRVIEYNGFFTGIAKIYSTKKWSRKNLNHEILASSKHIE
                     HHCAHFNQGGATMKKRIIPSVCSGFILSCMTQIAIADDIPTRSLPMSQILQHLLSKGY
                     HTIQEVEFDDGLYEVEALDEQGNQIILRMNPQTGDLIKNQNTQKYPISLLEAAQKIEG
                     AGYHSIYKIESHGSQYEVKALGKEDTIVKLRVDANTGKINRDE"
BASE COUNT          947 a          587 c          531 g          862 t
ORIGIN      
        1 gctcgctgca acaaagcgtc ccgtgctgct ttattttcct ggattttggc ctcgcccaat
       61 cgtacttgcg ccaatcgcaa atccagctct gccccagcac ctgccttaac ccggcgctca
      121 atagccgcca caatatcctg attcagtcga gtcagttttt tggtcacttg atgccattga
      181 ccggcgtata gcgcatccac ataagccaca cccacagcca tatacagcgc tgtttgctga
      241 actctgatgc tggcaagaga cgccagatag tctgcatagg ttgctttttt caaatactga
      301 agtcgatggc ctaaaggaat cggttgagtg actgaaaacg tggtttccgc agattcataa
      361 ctggagtagg cgccagagcc accgaaattc tccgccgtta aagtcagttg cggattggga
      421 taaaggccac tttgaataaa gtaaccccgc atggcccttg ctttgtccat ttcggcttgc
      481 aattcaggat tatttcgata agccgtgtcc aaagcctgtt taaaagtaaa gggggtagcc
      541 gccagtactt cgcaaacaac gaagtacatc atcatgaaaa tgacgagacg tgaaaaataa
      601 cgcattcctt accttttaag aaaatccatg tcaaacctta ttgtttcatt gtaccttgat
      661 ttcgtgtctt gatacaccct attgctaaaa tcaagacaat ctgatgctaa tgcactgaaa
      721 tttttctcaa attgagatac actgcgtttt atggtccctc gtcattttat cccatggtga
      781 tacgattatt ctttatttta taaatactcg ttaccgctga actaagggct caatcattaa
      841 ccattgggat tgtagcaaac gactcacgcg cagataaaaa tcattttttt ggatttgata
      901 tcgaactcat gacggaattg tgcaaacaaa tcaacatcca gcgtcaattt caagcccatg
      961 ctgtttaacg cgctgtttac ggacttaaat gccaggtaaa attgacctcg ccattgcagc
     1021 aattactatt accgacgaac gacaacaagc ctttcttttt agtcttccct atttagccag
     1081 tgaagcacgt tttgtcacca ccaaaaacaa ttcgctcaag agccttaaaa agcttaccca
     1141 taagaaaaca ggtattatca gggactctct ctttaaacaa ttaattactc agaaattcaa
     1201 atccaactcg attaaagaat acccttcgac agataaatta ataaccgcct taaatcaata
     1261 tgaagtggat gttctgctta tggatgattt ttctgccgat tattggatag caaataacaa
     1321 ccggttattt aaaggagttg gggacaccat tccgattgga ataggttatg gtatcatggc
     1381 taaaaaaaat tcgatggcgc taattaacaa aatgaatcaa gccctaaaac aaattgaaag
     1441 aaatggaatt tacttgtcct tgtataacaa atactttcat acgataccca tgcactaatt
     1501 gtctatacta attagtgtta attaggtcct ttctaacgag ttccacgccc atggacatga
     1561 ggatgatagc caccaaatcg cttcttaacc ggcgtgttac tttttgagtc ctctttagaa
     1621 acggaagcgc tgcaggaaaa caataaaaag gataaacaaa ctaaaagaac ttccattaat
     1681 ttttttacta aagtgaagtg tttcatgtat tttcttcctt tttattaagt ttgattattg
     1741 aatagtaagc gacagtctgt tgattgtggc atttaaaacg cttttagcca aatcctgagg
     1801 catcaaatgg aaagattact gatacgagtt attgagtata atggtttttt tactggtatc
     1861 gctaagatat actcgactaa aaaatggtct cggaagaact taaatcatga gatacttgcg
     1921 tcatcaaagc acatcgagca tcattgcgct cattttaatc aaggaggagc aactatgaaa
     1981 aaacggatta ttccatcggt ctgcagtgga tttattttat cctgtatgac acaaattgct
     2041 atcgctgatg acattccaac tcggtcattg cccatgtcgc aaatcctgca acacttgctg
     2101 agtaagggtt accatactat acaagaagtt gagtttgatg acggattata tgaagtagaa
     2161 gcccttgatg aacaaggaaa tcagataata ttacgaatga atccacaaac gggcgatctc
     2221 atcaaaaatc aaaacactca aaaatatcca atatccctat tagaagccgc ccagaaaatt
     2281 gaaggggctg gttatcattc tatttacaaa atagaatctc atggtagcca gtacgaagtg
     2341 aaagcccttg gcaaagaaga tacgatagta aaattaagag tagatgccaa taccggtaaa
     2401 attaatagag acgaatgatt cgattaggcg tattaattaa aatgcctaaa gacagctgat
     2461 ttttctttca ataagtagtt ataactgaca ctacttgttt ctaaacggtg ctatattatt
     2521 aagaaagatt tataagatga gatggattgt ttttctattg atttcacaat cactttattt
     2581 cattgcaagt agcatggata tatgccaaaa cagagatttt ttaaagccgt tattggggta
     2641 atggccttat tcagcaaaat gcttctgttg gcctcttgtg ccccatcaac aacgaccaat
     2701 gattagtaac agcggattaa ttattttaag cagattatta ccacctatca aaaacaagag
     2761 aaagaagtag ccgaaaacat catggcaagt gacaagggac tttagctcgc aaatcaggta
     2821 cgcattttaa ccatcactat acaaaatggc gaaatttatt ttactcaatt gaaagcgaca
     2881 ttaattttat atttttacaa ttaatacttt tattcataat caaagta
//
LOCUS       DACTJE010000238         2911 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.240, whole genome shotgun sequence.
ACCESSION   DACTJE010000238 DACTJE010000000
VERSION     DACTJE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.240"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..183
                     /locus_tag="I9760_002901"
     CDS             <1..183
                     /locus_tag="I9760_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAT5044638.1"
                     /translation="SGELAWWFWGGVHVFFLVGSRNRLSVILNWFWSYYTFRANNLLI
                     TDDSLNKKNKSGTSEV"
     gene            225..989
                     /locus_tag="I9760_002902"
     CDS             225..989
                     /locus_tag="I9760_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HAT5044639.1"
                     /translation="MDKYLSANSLEGVIDNEFSMPAPRWLNTYPAGPYRFINREFFII
                     AYETDPDLLQAILPPDMELLEPVVKFEFIRMPDSTGFGDYTESGQVVPVRYKGEEGGF
                     TISMFLDCHAPIAGGREIWGFPKKLAKPKLFVEEDTLIGILKYGSIDIAIATMGYKHR
                     PLDAEKVLESVKKPVFLLKNIPNVDGTPLVNQLTKTYLTDVTVKGAWTGPGSLELHPH
                     ALAPISNLYIKKIVSVSHFITDLTLPYGEVVADYLA"
     gene            1026..1298
                     /locus_tag="I9760_002903"
     CDS             1026..1298
                     /locus_tag="I9760_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HAT5044640.1"
                     /translation="MNKRPIQNTAVIAAALGAALASIYTYTDWLSSDEITVKRERCYN
                     VARAGKNDCATSQHSCAAQSTADRDPEAFIMLPKGLCERIVGGRSG"
     gene            1291..2604
                     /locus_tag="I9760_002904"
     CDS             1291..2604
                     /locus_tag="I9760_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HAT5044641.1"
                     /translation="MDSYSKLPLRIRKEIEAQQYTGELLVAGVQLGVIVLLSIINIFS
                     SAGYSPGAPVQSSSLGLWLFAILILLRLWFVYTNQLNPIFLGFFIVAEMALLLFIIWT
                     YYLQFETTPTINLKNPHINYVYVLIALRALRFEPVWVILSGLTAALGWGIIAWQTLSS
                     SGMNVITWDYVTFASTRSVYLGAVFDVILSVLLVTTIIALVLTRAKHTLFQAVEQTSA
                     AKDLSRFFDTSVVEKITTSEKSLQAGYGELRQAAIMFIDMRGFTKASVKLSPSELIGL
                     LAEYQRLLVPIIQKNNGSIDKFMGDGILASFGAVTPSNTYAADALRTVDEIVASVDVW
                     SENRRKKEQIVVGVGIGLASGEVVVGVIGYENRLEYTVIGEAANLAAKLEKHNKEEKT
                     QALTTRDTFLEAVKQGYEDKITKKELNARNVGGVGEPLDLVVLAK"
     gene            complement(2624..>2911)
                     /locus_tag="I9760_002905"
     CDS             complement(2624..>2911)
                     /locus_tag="I9760_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HAT5044642.1"
                     /translation="PTETGSIDFQQIPSYAVISRQKNQTVIHWITHDLFHFLCHIKAG
                     ASLGFAYENTLENNPDFDLLTALQFLLRNELLEDSFIHSNYHLTHANNCPE"
BASE COUNT          841 a          510 c          661 g          899 t
ORIGIN      
        1 agtggggaac tggcatggtg gttttggggg ggagtccatg tctttttcct ggttggctct
       61 cgaaatcgtt taagtgtcat tttaaattgg ttttggtctt attacacttt tcgcgccaat
      121 aatttgctga ttaccgatga ttcattgaat aaaaaaaata aatcagggac gagcgaagtt
      181 tagggctcat tgaagataat aaggaaatac aaagggagtg attaatggac aagtatcttt
      241 cagcaaattc tctagaaggg gttatcgata atgaatttag catgccagct ccacgttggt
      301 taaatactta cccggctggc ccatatcgat ttattaatcg tgaatttttt attattgctt
      361 atgaaaccga tcccgatctt ttgcaagcta ttttacctcc tgatatggaa ttattggagc
      421 cggtagtcaa atttgaattt atacgtatgc ccgattcaac aggatttggt gattacaccg
      481 agtcagggca agtggtccct gtgagatata aaggagaaga gggcggattt accatttcaa
      541 tgtttcttga ttgccatgcc cctattgctg gtggccgaga aatatggggt tttccaaaga
      601 agctggccaa acccaaattg tttgttgaag aagacacgct cattggcatt cttaagtatg
      661 ggagtattga tattgccatc gcaactatgg gatataaaca tcgtcctctg gacgcggaaa
      721 aggtattgga atccgttaaa aagcctgtat ttttacttaa aaacattcct aatgtagatg
      781 gaactcctct agtgaatcag ttgaccaaaa cctatttgac tgatgttaca gtgaaaggag
      841 catggaccgg gccaggtagc ttggagcttc atcctcatgc actggctcct atctctaatc
      901 tttatattaa aaaaattgta tccgtttcac attttattac tgatttgacc ttaccgtatg
      961 gagaggttgt tgccgattat ttggcctaaa ataaaaagcg actctaaaaa tggcaggaaa
     1021 atcatatgaa taaacgtccg atacaaaaca cggccgtcat tgcagctgct ttgggagctg
     1081 ctcttgcttc tatatacacc tatacagatt ggctttcatc agatgaaatc acagtaaaaa
     1141 gagagcgttg ctataatgtg gcgagagcgg gcaaaaatga ttgcgctact tcccagcatt
     1201 cttgtgctgc gcaatcaacg gccgatcgtg atcctgaagc atttatcatg ttacctaaag
     1261 ggttatgcga acgcatagtg gggggaagga gtggatagtt actctaagtt accgcttcgc
     1321 atcaggaaag agatagaagc ccaacaatac actggtgaac tattggttgc cggcgtccag
     1381 ttgggtgtca ttgtattatt aagcattatc aatatatttt cttcagctgg ctattcgcct
     1441 ggcgcgccgg tgcaatcctc atctctaggc ttatggctat ttgctatttt gatattacta
     1501 aggctgtggt ttgtctatac taatcaatta aatccaatat tcttgggatt ttttatagtc
     1561 gccgaaatgg ctttgttatt atttattatt tggacttatt atttgcagtt cgaaaccaca
     1621 cctaccatta atcttaaaaa tccgcatatt aactatgttt atgttttaat tgctttgcgt
     1681 gcgcttcgat ttgaaccagt atgggtaatt ctctctggcc tcactgcggc ccttggctgg
     1741 ggaattattg cctggcaaac tttatccagt tcgggtatga atgttattac atgggattat
     1801 gtgacattcg cgtcaactcg gagtgtctat ctgggagctg tatttgatgt catattgtcc
     1861 gttctactgg ttactaccat catagccttg gtattgacgc gtgcgaaaca tactctgttt
     1921 caagcagtag aacaaactag cgcggcaaaa gatttatcac gtttttttga tacaagtgtg
     1981 gtcgaaaaaa taacaacatc tgaaaagtca ttgcaagctg gatatggcga gcttcgtcag
     2041 gctgcaatta tgttcattga tatgcgaggg tttaccaaag catcagtgaa actgtccccg
     2101 agtgaactaa tcggactgct agcagaatat caacgcctac tggttccgat tattcaaaag
     2161 aataatggca gcattgataa attcatgggg gatggtattt tggcaagttt cggtgctgtg
     2221 accccttcaa atacttatgc ggcagatgca ttaaggactg tggatgaaat tgtcgcatcg
     2281 gtagacgtat ggagtgaaaa tcgccgtaaa aaagaacaga tagtggtcgg tgtaggaatt
     2341 ggtcttgctt caggtgaagt agtagttggt gtcattggtt atgaaaatcg tttggaatat
     2401 actgtaattg gcgaagctgc caatttagct gcaaagcttg aaaaacataa taaggaagaa
     2461 aaaacccagg ctttgactac ccgggatact tttttagagg cggtaaaaca aggttatgag
     2521 gacaaaataa ctaaaaagga gttgaatgca agaaatgtag gtggtgtggg agaacctttg
     2581 gatttagtgg tattggcaaa ataacccatg ttgctcttca cggttattca ggacagttgt
     2641 tagcgtgagt caaatgataa ttagaatgaa taaaagaatc ttccaataat tcatttctta
     2701 gtaaaaattg taatgcagtg agcaaatcaa aatcgggatt attttccaga gtattttcgt
     2761 atgcgaaccc aagtgaggcc cctgctttga tgtggcataa aaagtgaaac aaatcgtgag
     2821 taatccaatg aatcactgtt tgattttttt gacgagatat cactgcataa cttggtattt
     2881 gttgaaaatc aatagaacct gtttccgtgg g
//
LOCUS       DACTJE010000239         2908 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.241, whole genome shotgun sequence.
ACCESSION   DACTJE010000239 DACTJE010000000
VERSION     DACTJE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.241"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1407
                     /locus_tag="I9760_002906"
     CDS             <1..1407
                     /locus_tag="I9760_002906"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HAT5044643.1"
                     /translation="AGGSGTRLWPLSRQNFPKQFLKLNGELSLIQQTMKRAMGLPGNQ
                     TIIVSNDAHYFICQDQLQDFNAQTTYLLEPCARNTAPAIACAAHYLANTVGKDAVMLV
                     LPSDHWIADDEIWLAAMLEGGQFASENNAIVTFGIKPDSPKTGYGYIEAGTTLSSDIK
                     QVLSFREKPDAHTAAQFVSKGNYFWNSGMFICRAGVYLEELEQFEANIYQFSQQALTL
                     AQHHHDFLRLDLDCFSQCKEESIDYAIMEKTDKAVVIPISIQWSDLGCWTAVADANKQ
                     DEQGNTLIGNVIAQDSHNCLINSEELLVTTVGIQDQIIVATSDAVLVADKRYSQQVKD
                     LVHSLRKDHPHLTQDHQRVPRPWGYYEVLAEGTSFKVKRLMVKPGAKLSLQTHQHRAE
                     HWVIVGGEAEVVNGNNTFRLSKNQSTYIPKNALHRLSNPHNEPLYVIEVQSGSYLGED
                     DIKRFDDIYLRKEVELAE"
     gene            1635..>2908
                     /locus_tag="I9760_002907"
     CDS             1635..>2908
                     /locus_tag="I9760_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2888 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044644.1"
                     /translation="MATSSLILQSYLKTLKTDEKSELRYEDKVEELLEYNSNAKGSDF
                     LTPLSFYLPIIENTDEVMLRYMSEENKQKLIRDLQVAYLLLLTQKKYEEEHQKYENIK
                     TYAQHIKRCEELLDHLNYNQVCKEQKRAPSPEHGYASDGYPVKYLSIFLGKELAEMIV
                     DSMDRKTKTIKESMGWFNEKRLYWVWASSLLKVILAALPDDFFNVGQATQVVKAPDPY
                     TGTLSWALYYFRFSLNMFLLLKHTISGPWMSEREKKTPWTERFLTQWDQRKFTLLNDS
                     IWATGNLICFFWLTGKGALGTWGDVLTLALLVFDISIAIWDYEEQKTRHNKEMLAYEE
                     DIKKLKQLISKAEEEPGKEDEKLRIIKEYELQLQGLMRAQKESERNWDLQKVSLYAGI
                     AYAVGLMLAFALLAAPFFPVSGPALLAITITGA"
BASE COUNT          937 a          604 c          601 g          766 t
ORIGIN      
        1 gccgggggct ctggcactcg actatggcct ttatcaagac aaaattttcc taagcaattt
       61 ttaaaattaa atggcgaatt gtctttaatt caacaaacca tgaaaagagc gatgggattg
      121 cctggcaatc aaaccattat cgtaagtaat gatgcccatt actttatctg tcaggatcaa
      181 ttgcaagatt ttaatgcaca aaccacttac ttgcttgagc cttgtgcaag aaatacagcc
      241 cccgcgatag catgcgcagc ccattatttg gcaaacacag taggaaagga tgctgtcatg
      301 ctggttttac cctcggatca ctggatagct gatgatgaaa tctggctggc cgccatgtta
      361 gaggggggac aatttgcctc agaaaacaat gcgatagtga catttgggat aaagcctgat
      421 agccccaaaa ccggttatgg ctacattgag gccggcacca ctttatccag tgatataaag
      481 caagttttga gctttagaga aaaaccagac gcgcacactg ccgcacaatt cgtcagtaaa
      541 gggaattatt tctggaatag cggcatgttc atttgtcgtg caggggttta tcttgaagag
      601 ctggaacaat ttgaagccaa catctaccaa ttcagtcaac aagcattgac ccttgcgcag
      661 catcatcatg attttttgcg ccttgatctg gattgttttt ctcaatgtaa agaagagtct
      721 atagactatg ccataatgga aaaaacagac aaagctgttg taatacccat ttcaatacaa
      781 tggagcgatc tgggctgctg gaccgctgtg gccgacgcga acaaacaaga cgaacaaggc
      841 aatactctca ttggaaatgt catcgcccag gatagtcaca attgcctcat taatagtgaa
      901 gagttgttag tcacaaccgt tggcattcag gatcaaatca tcgttgcaac gagtgacgct
      961 gtgttagttg ccgataaacg ctattcgcaa caagtgaaag atttggttca ctccttacgc
     1021 aaagatcatc ctcatttaac tcaagatcat caacgtgttc ccagaccatg ggggtattat
     1081 gaagtgctgg cagagggcac ctcattcaaa gtcaaacgtc ttatggtgaa gcctggtgca
     1141 aaactctctt tacaaacgca tcagcatcgg gccgagcatt gggttatcgt tggcggtgag
     1201 gccgaggtag ttaatggaaa caatacgttc cgattatcaa aaaaccaatc cacctacatt
     1261 ccgaaaaacg cattgcatcg attaagtaac ccacataatg aaccacttta tgttatcgaa
     1321 gtacaaagtg gctcctatct gggcgaagac gacatcaaac gctttgatga tatttattta
     1381 agaaaggaag ttgaactggc agagtgaatt ctatccaaaa cagctctctt gattccactc
     1441 actgtgctta gcactcttgc ctaagttgca atacacttta tccctattcc tgcaaatttt
     1501 agtatcaata ctgtaaagcc aaccgcattc atcttgtacc caaaataaat actatgttat
     1561 aaaaaagaac aaatttgctt tttatagtat catttatata ggtaattaac aaaaattaaa
     1621 aaagagatga ctttatggca accagtagtt taattcttca atcttacctg aaaaccctca
     1681 aaacagatga gaaatctgaa ctacgctatg aagacaaagt agaagaactt cttgaataca
     1741 atagcaatgc caaaggaagc gattttctaa cgccactgag tttttatctg cccattattg
     1801 aaaatactga tgaagtcatg cttcgctaca tgagtgaaga aaacaagcag aaactcatac
     1861 gagatctgca agttgcctac ttacttttac tcacacaaaa aaaatacgag gaagaacatc
     1921 agaaatatga aaacataaaa acctatgcgc agcacattaa acgatgtgaa gaactgctag
     1981 accacctcaa ttataatcaa gtctgcaaag aacaaaaaag agcgccctct ccagaacatg
     2041 gctatgccag cgatggttat ccagtaaagt acttgagcat ttttttgggt aaagaactgg
     2101 cagaaatgat agtcgattct atggatcgca aaaccaaaac catcaaagaa tcaatgggct
     2161 ggtttaatga aaaacgtttg tattgggttt gggcatccag cctgctcaaa gtgatactcg
     2221 ctgcattacc tgatgatttt ttcaatgtcg gtcaggcaac ccaggtagtc aaagcccctg
     2281 acccttatac tggaaccctg agttgggctt tgtattattt tcgcttttcc ctcaatatgt
     2341 ttttgctact caaacataca attagtggtc catggatgag tgagagggaa aaaaagacac
     2401 cgtggacaga acggttcctc acccagtggg atcaaagaaa attcaccttg ctcaatgact
     2461 ccatctgggc aacaggaaac ttgatttgct ttttctggct tactggcaaa ggcgctctcg
     2521 ggacctgggg tgatgtattg actttggcat tgctagtctt tgacatcagc atagccattt
     2581 gggattacga agagcaaaag actcgccaca acaaagaaat gctggcttat gaggaagaca
     2641 ttaaaaaatt aaagcagtta attagcaagg cagaagaaga acccggaaaa gaggatgaaa
     2701 agcttagaat aatcaaagaa tatgaattgc aattgcaagg tttaatgcgg gcgcaaaaag
     2761 aatctgaaag aaattgggat ctgcaaaaag tcagtttata tgcaggtata gcttatgctg
     2821 tcggactcat gctcgctttt gcattactgg ctgcgccttt cttccccgtt tccggaccgg
     2881 ctttactggc aatcaccata acaggcgc
//
LOCUS       DACTJE010000240         2900 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.242, whole genome shotgun sequence.
ACCESSION   DACTJE010000240 DACTJE010000000
VERSION     DACTJE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.242"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(193..2073)
                     /gene="parE"
                     /locus_tag="I9760_002908"
     CDS             complement(193..2073)
                     /gene="parE"
                     /locus_tag="I9760_002908"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAT5044645.1"
                     /translation="MSENYTAEAIEVLSGLEPVQRRPGMYTDTTRPNHLAQEVIDNSV
                     DEVLAGFASRIIVTLHEDGSIEVEDDGRGMPVDLHPQLGLSGVEVIMTRLHAGGKFSD
                     KNYSFSGGLHGVGVSVVNALSERLDVTIKRNGIIYQMTFANGDKLCELNETGVTKKRE
                     TGTVIRFWPNGKYFDTTKISVKHLTHVLRAKAVLCSGLSMTFINKITNEEMNWCYEQG
                     LTDYLQQTLPEDYLPEEPFAGAFKSDEATVDWALAWSDSANGNLNESYVNLIPTIQGG
                     THVNGLRSGLFEAMSEFCELRNLLPRGVKLTADDLWESCQYVLSIKMKEPQFAGQTKE
                     RLSSRQTSGLVSNVIKDAFAIWLNQHRNQGEAIAALAIERAQKRLKQAKQVARKRVSQ
                     GPALPGKLADCLQTDLSQAELFLVEGDSAGGSAKQARNKDYQAILPLRGKILNSWEVD
                     SSQVLASQEIHDISVAIGVDPGSSDLEGLRYGKICILADADSDGAHIATLICALFLRH
                     FKPLVKAGHVFVAMPPLYRIDAGKLIHYALDDEEKNRIIQHLSQTTKSKVNVQRFKGL
                     GEMNPIQLRETTMDPNTRRLVQLTLDDEEQTEAIMDMMLNKKRAADRKLWLETKGNLA
                     EI"
     gene            2207..>2900
                     /locus_tag="I9760_002909"
     CDS             2207..>2900
                     /locus_tag="I9760_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5044646.1"
                     /translation="MQNTAKINQLSVAFKSPDKTVLALDKLSFSLNPGETLVLLGESG
                     CGKSLTSLAMMRLLPRAGVYGIDSQINIHGEDILNLPEKMMRELRGRRLAMIFQEPMT
                     ALNPVLTIGEQIAEVLKRHRSLTKEQIKERVILLLNEVEMPQPQMRIYQYPHQLSGGQ
                     KQRVVIAMALACEPDILIADEPTTALDVTIQAQILALLKKIQKKHQMSLLLITHDLGV
                     AKTMADRVCVMYA"
BASE COUNT          807 a          609 c          603 g          881 t
ORIGIN      
        1 agctagcaca agggttgatt tttagttagc ttcccgcctt cgcgggaaag acaactcaaa
       61 taaataggct tataagcaga aaaatttgca taccccgtgt acggttacta tccatattac
      121 caaggataag atagaaaagt agccgataat ggctatcgct aaaggggtgg ccaattgcta
      181 atgcacctta gctcaaattt cagcaaggtt tccttttgtt tctagccaga gttttctgtc
      241 ggcggccctt tttttgttga gcatcatatc cataatggct tccgtctgct cttcatcatc
      301 aagagtaagc tgtaccagac gccttgtatt gggatccatt gttgtttctc taagctgaat
      361 gggattcatt tcacctaatc ctttgaatcg ctgcacattc acttttgatt ttgtggtttg
      421 actaagatgt tgaatgattc tatttttttc ttcatcatcc agggcatagt gaatcagctt
      481 acctgcatcg attcgataga gcgggggcat ggctacaaaa acatggcctg ctttaaccag
      541 aggtttgaag tgtcttaaaa acaaagcaca tattagtgtg gcaatatgag ctccatcaga
      601 gtctgcatca gcgagaatac agattttccc atagcgaaga ccctcgagat cgctggagcc
      661 agggtctaca cctatagcaa cagatatatc atgaatttcc tgggatgcca gaacttgtga
      721 agaatctact tcccatgaat taagtatttt tccacgtaaa ggtaaaatgg cttgataatc
      781 tttattgcgg gcttgtttgg ccgaccctcc tgccgaatct ccttcaacca ggaatagttc
      841 cgcttgactt aagtccgttt gcaagcagtc ggctaatttt ccaggtaaag caggtccttg
      901 gctaacacgt ttgcgtgcca cttgtttggc ttgctttagc cgtttctgtg cgcgttctat
      961 tgcgagagct gctatggctt caccctggtt gcgatgctga ttcaaccaaa ttgcaaaagc
     1021 gtcttttatg acattactga ctagtcctga ggtttggcgt gaactcaagc gctctttggt
     1081 ttgccccgca aattgcggct ctttcatttt gattgataaa acatactggc atgattccca
     1141 tagatcatcc gctgtcaatt ttactccacg aggaagaagg tttctcagct cacaaaattc
     1201 agacatggct tcaaataatc ccgaacgcaa tccattgacg tgcgtgccac cttgtattgt
     1261 cgggattaaa ttcacataac tttcatttaa attgccattg gcagagtctg accaggccaa
     1321 agcccaatca acagttgctt catcactttt gaatgctccg gcaaaaggtt cttcaggcaa
     1381 atagtcttcg ggcaaggttt gctgtaaata atcagttaaa ccttgctcat aacaccagtt
     1441 catttcttca ttagtgattt tattgataaa ggtcatggac aagccactac acaataccgc
     1501 ttttgctctt aatacatgag tcagatgttt tacagaaatt tttgtagtgt caaaatattt
     1561 gccattaggc caaaaacgta taacagtccc ggtctctctt ttttttgtaa ccccggtttc
     1621 atttaattca cataatttgt cgccattagc aaaggtcatt tgataaatga tcccattgcg
     1681 ctttatggtg acgtcaagtc gttctgacaa ggcatttacc acggacaccc caacgccatg
     1741 caagccgcca gaaaagctgt aatttttatc tgaaaactta cctccggcat gaagtcgagt
     1801 cataatgacc tcgacaccac ttaagcccag ttgaggatgc aaatcgactg gcatacctcg
     1861 gccatcatcc tcgacttcaa tagagccgtc ttcatgtaaa gtaacaataa tacggctggc
     1921 aaatccagca agtacttcat cgacgctatt atcaatcact tcctgagcca aatggttggg
     1981 gcgtgtcgtg tcggtataca taccgggacg tcgttgaaca ggctcaagac cacttaagac
     2041 ttcaatcgct tccgcagtgt agttttctga catgggtatt cactcaaata ttaattgtgc
     2101 ttattctatc ataaacattc ttttgctcag agtggtatgg tatttttatt gctactatca
     2161 cccctctctt gttagagtgt agttatcatt atttgtggca aactccatgc aaaacaccgc
     2221 taaaattaat caattgtcag ttgcttttaa aagccctgat aaaacagttc tggctttgga
     2281 taaattaagt tttagtctaa atccagggga gactttggtt ttactgggag aatcaggatg
     2341 tggtaaatca ttaacttcat tggctatgat gagattactt cccagagctg gtgtctatgg
     2401 gattgacagc caaatcaata ttcacggtga agacatctta aatttacctg agaaaatgat
     2461 gcgggagtta agagggcgca ggcttgcgat gatttttcaa gagccgatga cggctttaaa
     2521 tcccgtatta acgattggag agcagatagc tgaggtttta aaaaggcatc ggtctttgac
     2581 aaaagagcaa ataaaagaaa gagtaatatt gttattgaat gaggtagaaa tgcctcaacc
     2641 acaaatgaga atttatcaat acccgcatca attgtctggt ggtcaaaagc agagggtggt
     2701 tattgctatg gcgttggctt gcgagccaga tatccttatt gccgatgaac ctactacggc
     2761 tttggatgtg actatccagg ctcaaatatt ggccttgcta aaaaaaatac aaaagaaaca
     2821 tcagatgagt ttgttattaa ttactcatga tctgggcgtg gctaaaacca tggctgatag
     2881 agtttgtgtg atgtatgcag
//
LOCUS       DACTJE010000241         2887 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.243, whole genome shotgun sequence.
ACCESSION   DACTJE010000241 DACTJE010000000
VERSION     DACTJE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.243"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(93..863)
                     /gene="ceg19"
                     /locus_tag="I9760_002910"
     CDS             complement(93..863)
                     /gene="ceg19"
                     /locus_tag="I9760_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg19"
                     /protein_id="HAT5044647.1"
                     /translation="MFKEIITKLGIVMSKLRALEKEANELALILDQALLSQSIEVKRR
                     ALSQFENSELIQNKDNLRVFIDPEHPIVSLIILATKTLPEKIENLQKKVRLLEIQLAA
                     ETEETLDESNSDSESVDYDNGIDVETVVPVSVTHQEHVSEPDTRSVAIARLREVLKPY
                     IDSTQERTYEHYYGMAGTLFSFFGAAGYSKTDKLNAITKLFQNLEGDGAEPLNDTDRD
                     ILTTGVLGTTMKPLLEDEHIGNELKDLLSIKTDNVNII"
     gene            1445..>2887
                     /gene="lem6"
                     /locus_tag="I9760_002911"
     CDS             1445..>2887
                     /gene="lem6"
                     /locus_tag="I9760_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem6"
                     /protein_id="HAT5044648.1"
                     /translation="MTTPLFPKNDGFVTIRQGKTGDCYLLATLDCILNSGPEGYAAIK
                     SLFKETKDGIEVRFKSSDLSQNLQKDKLQGKYKYHYDPINNQDVFFIDKKKIQEIDQT
                     TDGVKSNSLAIKILERLSSYYLASDWNPNLPLASIVAHNGSKGSKRHNETSTKFVGQL
                     LGFMAHDTRDIHEIIRFKTTAPQEPVYISIKYGEVDSNGVRHGRHALRLDRIIPNPKS
                     AGGYDFVLVNPWNNQLKETYSLDEIQQRKCRFSIFSTKPYKNDLKPYEHDLKEKLFKV
                     YQKLGKKIFDNDKLYQMILKMSEVNGKLLDLRTISKCVKLYERISGFPNLFAALSLSE
                     QKAMISYVDLESKPTKFIRSFLSAAPTARSAVFEHLDLTKYYVNKISQYPIESVNFDD
                     SIESVNKYKESVISELQKIKGHALAQKKYTQLDSAIIEKAFAEKLREVDALLRFGLFF
                     KPQNIDLNQGLNVEPNQDLNIDLNQALNVEP"
BASE COUNT          982 a          480 c          492 g          933 t
ORIGIN      
        1 cctaagatgc cggatcattg atccggcatc ggatggcttg atgagataag tgtttcagat
       61 gaacaggaaa actcctgttc aaatatgagt ttttaaatta tattcacatt atcggttttt
      121 atcgatagca agtctttcaa ttcattacca atatgctcat cttctaacaa cggcttcatg
      181 gtagttccaa gaacccctgt agtcagtata tctctatccg tatcattgag tggctcagcg
      241 ccatccccct caagattttg gaacaatttc gtgatagcgt ttagtttatc cgttttactg
      301 taacctgccg ctccaaaaaa actgaacagt gttcctgcca ttccataata gtgttcatat
      361 gtcctttctt gagtagaatc gatataaggt ttcaacactt ctcttagtcg tgcaatcgcc
      421 acagacctgg tgtcaggctc actgacatgc tcttgatgag taacgctgac aggaacaaca
      481 gtctctacat caataccgtt atcgtaatca acgctttcag aatccgaatt cgattcatca
      541 agggtttcct cagtttcagc agccaattgg atctcgagca aacgtacctt tttttgcaga
      601 ttttctattt tttcaggtaa agtctttgta gcaagtatga ttaagcttac aatgggatgc
      661 tcagggtcaa taaaaacccg caaattatct ttgttttgta ttaattctga attttcaaat
      721 tgagataatg ctcttctttt aacctctatt gactgactca acagggcctg atcaagaatg
      781 agagccaatt cattcgcttc tttttccaat gctcttagtt tagacataac tattcccaat
      841 ttagtgataa tttctttaaa cagtgattaa ttataaaaca aaaacattaa gataatctta
      901 acttatgaaa aatgggctat ttttccgatt cgtaaatttt ccttcggaat ggtacttgtt
      961 agagcttttg taaaatagtt ttacgggagt gttctcaagt tctattggtt tgacttttgt
     1021 cttcctgtgg ttgatgccat tcataaccaa ataagtcaga ttattatggg gttctgcctt
     1081 tttgaattaa acagagttca tgatttatct attggtactg aaaattccga taacatctac
     1141 aaggataaat ttatgtatta tttttgagca caagcaacct taaatagagt tatattacac
     1201 atcatttgtc gcaaaaacga tcttcatgaa aatatttaat taatattgat taattaatat
     1261 acagttatct atgaatagtt ggatagctgt ttaagcaaga aagtgagttg attattcaat
     1321 taacttattg aaataaagaa cattcatcgg tcctgtaatg gcataagaat tcatagagtt
     1381 taaatcgaga aaatttatac gagtaactta aattttcaac ttagaatttt taggagatta
     1441 aatcatgaca acaccactat tccccaaaaa tgacggtttc gtaactatac gtcaaggtaa
     1501 aacaggagac tgttatcttt tggcgacttt ggattgtata ctaaattcgg ggccagaagg
     1561 ttatgcagcc atcaaatcat tatttaaaga aacaaaagat ggcattgaag taagatttaa
     1621 atctagtgat ctgagtcaaa atttacaaaa agataagttg cagggtaaat acaaatatca
     1681 ttacgatccg atcaataatc aagacgtgtt ttttattgac aaaaagaaaa tacaagaaat
     1741 tgaccaaact actgacggag taaaaagtaa ctctttggcc ataaaaattt tagaacgctt
     1801 aagttcttat tacttggcat cggattggaa tccaaatcta ccattggcaa gtattgttgc
     1861 tcataatgga tcaaagggat caaaaaggca taatgagacc tcaacgaaat ttgtcggtca
     1921 attattgggt tttatggctc atgatacccg tgatattcat gagatcatac gatttaaaac
     1981 tacagctcct caagagccag tttatatcag tataaagtat ggagaagttg attctaatgg
     2041 ggtaagacat ggcagacatg ctttgagact tgatagaatt attcctaatc caaaaagtgc
     2101 gggtgggtat gattttgtct tagtcaatcc atggaataac caactgaaag aaacttatag
     2161 tttagatgaa atccaacaaa gaaaatgtcg tttctctata tttagcacca agccttataa
     2221 gaatgatctt aaaccttatg aacacgatct taaagaaaag ctattcaaag tctatcaaaa
     2281 attagggaaa aaaatatttg acaatgataa actttatcag atgattctaa aaatgagcga
     2341 agtgaatggc aaattattag atttaagaac aattagcaaa tgcgttaaac tatatgaaag
     2401 gattagtggt tttcccaatc tgtttgccgc gttatccctt agcgaacaaa aggcaatgat
     2461 ttcatatgtt gacctagaat caaaaccgac gaaatttatc agatcattcc tatctgcagc
     2521 accaactgct cgctctgcag tttttgaaca tctagattta actaaatatt atgtgaataa
     2581 gattagtcaa taccctatag aatctgtgaa ttttgatgac tctatagaat cagttaataa
     2641 atacaaagaa tctgtaatat ctgaactgca aaaaataaag ggccatgcat tggctcaaaa
     2701 aaaatatact caactagata gtgctattat cgaaaaagct tttgctgaga aattacgtga
     2761 ggtagatgca ctgttacggt ttgggttatt ttttaaacca caaaacattg atctaaatca
     2821 aggtttaaat gttgagccaa atcaagattt aaatattgat ctaaatcaag cattaaatgt
     2881 tgagcca
//
LOCUS       DACTJE010000242         2881 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.244, whole genome shotgun sequence.
ACCESSION   DACTJE010000242 DACTJE010000000
VERSION     DACTJE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.244"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..706
                     /locus_tag="I9760_002912"
     CDS             <1..706
                     /locus_tag="I9760_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT5044649.1"
                     /translation="KYTGWPEKNIEAFKLDDMKVLFSGEPQWDVEEGPLFNKDKEVSY
                     YRTRRVPLFDKDNKVYGLVVVLTDITKFKKMEEQLQKVATKEKIKPSPAKRIEEPKIL
                     MVEDNFVAQKVEEALLKELQCQVDIADTGDKALNLFDPGKYDMVFMDIGLEDTSGYLV
                     AKKIRQKEQNTNYHVPIIALTSYQADVVKYDVEDYFMDGVLTKPLTSEQADQLIKHFV
                     HKEDIMVSGLKSAREN"
     gene            988..1749
                     /locus_tag="I9760_002913"
     CDS             988..1749
                     /locus_tag="I9760_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5044650.1"
                     /translation="MIWDPERYVLGNYIQNKINDVFLKTIHSKLSGNILDIGCGDGHY
                     TSLLAGKVKHGQILGIDSSEQMIMHANQQWARTGLSFEVHNIEEFHQPQSFDLVLSFW
                     CLHWTNIHISFPNIFHLLKREGKLYAVMSSFSDHSILQTWKELAKQNLYRDLTKQYIS
                     PINEQYFYGVINLLNRLPFKRIKLDLKTCRVHLPHIDYYKNLLLTMPFIKRVPSEITD
                     TLVEDMLKAFQTICDRKYGGELYYETRPIFLEAIK"
     gene            1774..2808
                     /locus_tag="I9760_002914"
     CDS             1774..2808
                     /locus_tag="I9760_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044651.1"
                     /translation="MHLNFNTTPGMNIPMVIASQDNLKASPTPLLHFISEYHDEIIQK
                     LNQYGAIVLRGFSCQEENCFSKAIELCALGTRCSTSDYDLPRTVLANEIYTSSDLPAD
                     IPLPLHHEKPRSPKPPNHIYFCCIIPPQEGGGTIFANAEEIWIDMPQGIQNKILEYGV
                     QYKQFFHGQSVKYRVLRKILGNHCARSWIDYFGTEDKTQIEQNLTQKQVVWDWINHGI
                     DLIIINYLPGALKHPLTDKMAWFNSSAYLNYYSNLNYGELKNLRSFKYWASRYLILKD
                     MLPMICHYGNGQAFSAKEISEINQVIQRHTRVFHWQKGDFMIVDNFTFMHGKQAHVGE
                     RLLYSCMTAY"
BASE COUNT          971 a          514 c          553 g          843 t
ORIGIN      
        1 aaaatacact gggtggccag aaaaaaatat tgaagcattt aaactggatg acatgaaagt
       61 gctcttttca ggagaacccc aatgggatgt agaagagggg cctttattta acaaggataa
      121 ggaagtgagt tattaccgaa ctcgtcgcgt accattattt gataaagaca ataaagtata
      181 tggattggta gttgttctaa ctgacatcac caaatttaaa aaaatggaag agcaattaca
      241 gaaagtagct acaaaagaaa agataaaacc tagtccagcc aaacgaatag aagaaccaaa
      301 aatattaatg gtcgaagaca attttgttgc acaaaaagtt gaagaggcat tgcttaagga
      361 actgcaatgc caagttgata tcgcggacac gggagataaa gcattaaatc ttttcgaccc
      421 ggggaaatat gatatggtct ttatggatat tggtctcgaa gatacttctg gctatttggt
      481 agcaaaaaaa attcgccaaa aggaacaaaa caccaattat catgtaccaa ttattgcctt
      541 aacatcttac caggctgatg tggtgaaata cgatgttgag gattatttta tggatggagt
      601 gctcactaaa cccttaacaa gcgaacaagc agaccagtta atcaaacact ttgttcataa
      661 agaagacatt atggtttcag gcttaaaatc agcaagagaa aattaagatc aacttgctta
      721 gctgacaata tctgattttt agcttgttcg catgaaacaa aatgaaacat tgccaacacc
      781 tggtaaatag agaaagtgtt aaattggcac atccgtgcac tacagcatta aagtacaacc
      841 tcgtcggcga ggactgtatc ctcataaaaa caagacacgt cccgcataat gtgtgagaat
      901 agatttcagc catgccaaag atatttgtaa tcgctggttt cttgggtata tcttccctta
      961 caatgattat tgatactgga gaatgaagtg atatgggatc ctgaacgtta tgtgcttgga
     1021 aactatattc aaaataaaat caacgatgta tttctaaaaa caattcacag caagctgtct
     1081 ggaaatattt tagatattgg ttgcggagat gggcactata ccagcttgtt ggcgggcaag
     1141 gtaaaacatg gccaaattct gggaattgat agctctgaac aaatgataat gcacgccaat
     1201 caacaatggg cacgtacggg gctatctttt gaagttcata acattgagga atttcaccaa
     1261 ccgcaatctt ttgatttggt gctctcattt tggtgtctgc attggacgaa tatccatatt
     1321 tcttttccaa atatttttca tcttctaaaa agggaaggca aattgtatgc tgtcatgtct
     1381 tccttttccg atcacagcat tttgcaaacc tggaaagaat tggccaaaca aaacctgtat
     1441 agggatttaa caaaacaata catctctcca atcaatgaac agtattttta tggtgtgatt
     1501 aatcttctca acagattgcc ttttaaacga attaaacttg atttaaaaac ttgccgtgtt
     1561 catttgccgc atattgatta ttataagaat ttacttctaa ccatgccttt cataaaaaga
     1621 gttccttctg aaataacaga tacactggtt gaggacatgt taaaagcatt tcaaactatc
     1681 tgtgacagga aatacggtgg tgaattatat tatgaaactc gccccatatt tttagaggct
     1741 atcaaataac tcaaggtgtt aggaggcaat actgtgcatt taaattttaa tacaactcct
     1801 ggcatgaaca tacctatggt cattgcaagc caggataatt tgaaagcaag ccctacccct
     1861 ttattacatt ttatttctga gtatcatgat gagataatcc aaaagctcaa tcagtatgga
     1921 gcaatagtat taagaggatt ttcctgtcaa gaagagaact gcttttcgaa agcaatcgaa
     1981 ttatgtgcct taggcacgcg atgttcgaca agcgattatg acctgccaag aactgtactg
     2041 gcaaatgaaa tttacacatc aagtgacctg ccggctgata ttcccttacc gctgcatcac
     2101 gaaaaacccc gatcaccaaa accacctaat cacatctatt tttgctgcat cattccgcct
     2161 caggaaggtg gtggaacaat ctttgccaac gctgaagaaa tatggataga tatgccccaa
     2221 ggcattcaga acaagattct tgaatacggt gtccaataca agcaattttt tcatgggcaa
     2281 tctgtaaaat atcgtgtatt aagaaaaatt ctgggaaacc attgtgctcg aagttggatt
     2341 gattattttg gcacagaaga caaaactcaa atagaacaaa atttaactca aaagcaggta
     2401 gtatgggatt ggataaatca tgggattgat ttgataataa taaattattt gcccggagca
     2461 ttaaagcatc ctctaactga taaaatggcc tggtttaatt catcagctta tttgaattat
     2521 tattccaatt tgaattatgg tgaattaaaa aacctgcgtt cattcaaata ttgggcaagc
     2581 cggtatctta tcctgaaaga tatgcttccc atgatctgtc attatggtaa tggccaggcg
     2641 ttttctgcaa aagaaatatc tgaaattaac caggttattc agcgtcatac tcgtgttttt
     2701 cattggcaaa aaggagattt catgattgtg gataatttta ctttcatgca tggcaaacaa
     2761 gctcatgttg gagaaagatt attgtattca tgcatgacgg cttattaatg ctggattatt
     2821 tttatctgct ccctgacagt cgcggttcag tgtttgaacc ataatccgag ccgcaactat
     2881 c
//
LOCUS       DACTJE010000243         2843 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.245, whole genome shotgun sequence.
ACCESSION   DACTJE010000243 DACTJE010000000
VERSION     DACTJE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.245"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..546
                     /locus_tag="I9760_002915"
     CDS             <1..546
                     /locus_tag="I9760_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0181 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044652.1"
                     /translation="ENQLNLPGRMEREKEHVALAVPFCSIKKITIHNKELANPFYLSI
                     PQENHEAKMELNTLYGELISLLRKKYTQEVDEKEEQIALRTYAIRYLDFYAKFCGCDN
                     PFDKTIAQLSELYPEFMSNFLQSSHFSSKTGLMKEIVVNSLDNLFKEHPYTKSIDASY
                     IYRVKESTTCYEDDWAKPVYD"
     gene            complement(593..1045)
                     /locus_tag="I9760_002916"
     CDS             complement(593..1045)
                     /locus_tag="I9760_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044653.1"
                     /translation="MLNSSSYMKKSSFFDYSFFTISDTKEEFPLFPEEGMDCLFEKLG
                     SRITSKKAGNTYFISPADPSIAIQKGNDGVHEYYHLLLNKKVIAVSSFVNHKTTIYLA
                     NIIKQLEPERIGTPVDFDFLGDTDETDSVFSDTLDNAEGNVDQTPSPR"
     gene            1374..>2843
                     /locus_tag="I9760_002917"
     CDS             1374..>2843
                     /locus_tag="I9760_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HAT5044654.1"
                     /translation="MTKKVTNIAIIGGGTGLTSAWALENQQKFHVTVFEENDRLGGHI
                     HSIKVNNVILEGGAEFIGPPTLYPNVHRLFQYLGVALDQFELNMDFDDLKQKDHIVLP
                     PLYHTSSGRSEDTSSILYTLSCGLFGKRRKQNETRVSVDTLLTEMYNLLEMNHLIMDA
                     RNKLLHPDNLITLEQFIKEFKEKCVHTLSPIDKFAEEFLYPLIASGWGVSIETIKTFG
                     AHYAMNYLAAGMNWFDAPEGLSSYIDRMVAQCTNTQFNANTAIKKLIPITIDGKIKYQ
                     LLKRDGTFVCDATGSPMIYDDVVISTPAYVTSELLSGIESNTIKVLREKLANVTYYDT
                     TVVFHQDPEYLSPYRTVVHSRFDGAQSANTMCKPWKFNNEETPIMKTWVLPGQAMPKN
                     VLREVHYKHPVMDKNYYEAQQALHAAQGEAGLWHGGILAGFNDSHESGITAALQVAAQ
                     LNHREHCLHKNERLALFPNLVDSVLSPKLSTGRVVEVEGV"
BASE COUNT          945 a          474 c          572 g          852 t
ORIGIN      
        1 gaaaaccagc tcaatttgcc tggccgaatg gaacgagaaa aagagcatgt tgctttagct
       61 gtaccttttt gcagcataaa aaaaataaca attcacaata aagaattagc taaccctttt
      121 tatctcagca tacctcaaga gaatcatgaa gcaaaaatgg agttgaatac gctctatggt
      181 gaattaattt cgcttttaag aaaaaaatac actcaggaag tcgatgagaa agaagagcaa
      241 atcgcattac gtacttacgc catacgttat ttagactttt atgctaaatt ctgtggttgt
      301 gataatcctt ttgataaaac cattgcacaa ctgtcggaac tctatccgga atttatgagc
      361 aattttttgc aatcaagtca tttttcatca aaaacaggtt tgatgaaaga gattgtcgtg
      421 aattcattgg ataatttatt taaagaacat ccttatacta aatcgattga tgcttcttat
      481 atctataggg ttaaagagag cactacttgt tatgaagatg actgggcaaa gccagtttat
      541 gattagtaat aagaaagatg ctggttttcc ttgaattcgt tgaagatgac tactaacgag
      601 gcgagggcgt ttgatctacg ttaccttctg cattatccaa agtatctgaa aacacggaat
      661 ctgtttcatc tgtatcaccc aaaaaatcaa aatctacggg cgtaccgatg cgttctggtt
      721 ccagttgttt aatgatgtta gctaaataaa ttgtggtttt gtgattaacg aatgatgata
      781 cagcaatgac ttttttatta agtaagaggt gataatattc atgaactcca tcgttccctt
      841 tttgaatagc aatagatgga tcagcgggcg agataaaata agtattgccg gcttttttag
      901 atgtaatgcg tgaccccagt ttttcaaata aacaatccat gccttcttca ggaaaaagag
      961 gaaactcctc tttggtatct gaaatggtaa aaaaggaata atcaaaaaaa gatgattttt
     1021 tcatatagga gctactatta agcataaata tgcccaaaat tatatcatga taaacaattt
     1081 aaaggtattt gaattgctaa tcaccgacgc taatgtaaca aatggaagaa tatacttgag
     1141 tagcaaatag agtaataacg ttgaggcaaa tttttgtagt aattcacgat gagcgtgcgt
     1201 tataaagtta tttgaaatga ggcggcagtc gtaaatggaa tacaaacaag tgcatgaaca
     1261 ttgtttttaa taatctccct ttatcttttc ccaattaatc acagaaagcg tatttacttt
     1321 tttgttctaa ccatttagtt tacgagcctg gtatttggaa atggactttt aaaatgacta
     1381 aaaaagtaac gaatattgcg ataattggtg gtggaacagg attaacttct gcctgggctt
     1441 tggaaaatca acaaaaattt catgttactg tatttgagga aaatgatcgc ttaggcgggc
     1501 atatccacag catcaaggtt aataatgtaa tcctggaagg gggggcagaa ttcatagggc
     1561 cacccacatt gtatcccaat gtgcatcgat tgtttcaata tctgggagtg gctttagatc
     1621 agtttgaatt aaatatggat tttgacgatc tgaaacaaaa ggatcatatt gttttacccc
     1681 ctttgtatca tacatcaagt ggcagatctg aggacacttc atcaattcta tatacattat
     1741 cctgtggttt atttggcaag agaaggaaac agaatgaaac ccgtgtttca gtggatactc
     1801 tactcactga aatgtacaat ttgcttgaga tgaaccactt aattatggat gccaggaaca
     1861 aattactgca tccagataat ttaataacat tagagcaatt tataaaagag tttaaagaga
     1921 aatgtgtaca cacactttca cctatagaca agtttgctga ggagttcttg tatcctttga
     1981 ttgcatcagg atggggagta tcaattgaaa caattaaaac atttggtgcc cattatgcta
     2041 tgaactatct ggctgcagga atgaattggt ttgatgcgcc agaaggtttg agcagctata
     2101 ttgatagaat ggtggcgcaa tgcactaata ctcaatttaa cgctaatacg gctataaaaa
     2161 aattaatacc aataacaatt gatggaaaaa taaaatacca attattgaaa agggatggca
     2221 cattcgtttg tgatgccact ggaagcccaa tgatttatga tgatgtggtt atctccacac
     2281 cggcttatgt aacgagtgaa ctgctgtctg gtattgaaag taacacgata aaagtattgc
     2341 gtgaaaaact tgccaacgta acctactatg acacgacagt agtatttcat caggatcccg
     2401 aatatctgtc tccctatagg acagttgtgc acagccgatt tgatggggct caatcagcaa
     2461 ataccatgtg caagccctgg aaatttaata atgaagaaac cccgataatg aaaacctggg
     2521 ttttgcccgg gcaagcaatg cctaaaaacg tactgagaga agttcattat aaacacccgg
     2581 taatggataa aaattactat gaagcacagc aagctttaca tgccgctcaa ggtgaggctg
     2641 ggttatggca tggtggtatt cttgctggat ttaatgattc gcatgaaagt ggtattacgg
     2701 cagcactcca agttgctgcg caattgaatc accgtgaaca ttgtttacat aaaaatgaac
     2761 gtctcgcact attccccaac ctggttgatt cggttctgtc tcctaaattg tctacgggta
     2821 gagtcgtgga ggttgaagga gta
//
LOCUS       DACTJE010000244         2822 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.246, whole genome shotgun sequence.
ACCESSION   DACTJE010000244 DACTJE010000000
VERSION     DACTJE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.246"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            62..1396
                     /locus_tag="I9760_002918"
     CDS             62..1396
                     /locus_tag="I9760_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044655.1"
                     /translation="MTPIISHLLKIFPELNTQVKTDSLNWTFNTHLKQLGPDFYSKVA
                     RLHPILNMEYSVLCQHLRYNLSSPDYSSPEQIKEQLIDALKLAELLEYTYQHYLVVPR
                     EVVRLRRHKAIYKELLTELSGYSFALDNPEPESLKTSLSLTQAIREKTAQSNWYRIFI
                     SRSKRVINLLDNLDTGSKAFRDFVVLLDKYTNPFLAYLGWCFFAPRLFTNLFLILKHT
                     IPGEWMGEKEKSLDWSDRFYAHLQRRWFELANDSVWFTVGILNCFVLVGALAPLSVYL
                     SLFAFVFDVANTSLRAYIEIGRLQQLQKEYGELFDQEENEDQKKIIKEYQDLISYRIK
                     FEILRSFLSVGGAVAVVIAMALSVPALGIINPIIPLIGAVLLLALWGVSLYLNNKLDE
                     YRPVDNIEKPAPSVINKLSFFAPKNEKKESLHLSSKPEKVLEAEELIPAFTF"
     gene            1495..>2822
                     /locus_tag="I9760_002919"
     CDS             1495..>2822
                     /locus_tag="I9760_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HAT5044656.1"
                     /translation="MSTTVGLPQFSHIKIEHFKSHLDALLKNHLEEIDRLLKENHHYT
                     WDNLIYPLDSLADELERFWSPFAHMHAVMDSETIRESYEACLPLLSAYDAAIGHNQDL
                     YEAIKSIDQHSLNPAQKKIIADSIQDFELSGVALSKANKKRFEAIQSRLAELSSKFEN
                     NVLDATHAYTIHITEAERMAGLPEHALNTAKELAHEKGLDGFVLTLEYPCFQAVITHA
                     EDRALREEMYRAYITRASDQGPNAGTFDNTPLIDEILSLRHEKAELLGFNNFAELSLA
                     TKMAASTNQVTEFIYDLISRTRDKGKAEFRQLEVFAQDKWNLSPVNPWDVAYLSEKRR
                     QDLYSLSQEELRPYFPQPKVMQGLFAIVKKLFGMSIEEIEGVDVWHKDVQCYCIVDES
                     NQTRGYIYTDLFARPHKRNGAWMDSMQSRRKLEDGTVQLPIATLTCNFAKP"
BASE COUNT          818 a          562 c          602 g          840 t
ORIGIN      
        1 aaatttttgc aaagctggat tagaaacaca atgatatgcg gattatttat ttcaaacaat
       61 catgacaccg attattagtc atttattaaa aatatttcca gagctgaaca cgcaagttaa
      121 aactgattct ttgaattgga cttttaacac gcacctaaag caattgggcc ctgattttta
      181 ttcgaaagta gctcgtcttc atcccattct caatatggaa tacagcgtat tgtgtcagca
      241 cttaaggtat aacctgtcat cgcctgatta cagttctccg gagcaaatca aagaacaact
      301 gattgatgca ctcaaactgg ctgaactttt ggaatatacc tatcaacact atctggtcgt
      361 tccgcgcgaa gtagtgcgtt tacgtcgcca taaagccata tacaaggaat tattgacgga
      421 attgtccggg tattcttttg ctttggataa tcctgagcct gaatctctca agacaagctt
      481 atcgttgacg caggccattc gtgagaagac tgctcaatcc aattggtata gaatttttat
      541 cagcagaagt aaacgagtca ttaatctttt ggataattta gacactggtt caaaagcatt
      601 tcgtgatttt gtcgtgctgc ttgataaata cactaatcca tttttggctt atttgggctg
      661 gtgttttttt gcccccagat tatttaccaa tctcttttta attttaaagc acaccattcc
      721 aggggaatgg atgggcgaaa aagaaaaatc actggattgg tccgatcgat tttatgccca
      781 cctgcaacga cggtggtttg agttggccaa tgacagcgtt tggtttacag tcgggattct
      841 caattgcttc gtgctggtag gcgctttggc acccctatct gtttatttgt ctttgtttgc
      901 gtttgtcttt gacgtcgcca acacaagcct gcgtgcttac atagagattg gtcgattaca
      961 gcagttgcaa aaggaatatg gcgaactctt cgaccaggaa gaaaatgaag accagaaaaa
     1021 gataattaaa gagtatcaag acttgataag ttatcgcatt aagtttgaga ttttgcgctc
     1081 ctttttatcg gttggcgggg cagtcgctgt tgtgattgca atggctttat cagttcctgc
     1141 tttgggtatt ataaacccaa tcatcccctt gattggggca gtgcttttgc tagcgctatg
     1201 gggcgtttca ttatacttga acaacaagtt agatgaatac agacctgtcg ataacattga
     1261 aaaacctgct ccttccgtta tcaataaatt aagctttttt gctcccaaaa atgaaaaaaa
     1321 agagagtctg cacctgtctt ccaagccaga aaaggtgctt gaggcagagg aattaattcc
     1381 agcgttcacc ttttaattca ttactgtact ttgccatttc accgctggta agtatagaga
     1441 ctaattcaat tgaatgttat gctttcaaaa aattacatga tggaaaagta taacatgtcg
     1501 accacagttg gattgccgca atttagccat ataaaaattg aacatttcaa atctcatctt
     1561 gatgctttat taaaaaacca tcttgaggaa attgacaggc tgcttaagga aaatcaccac
     1621 tatacctggg ataatttaat ttacccttta gatagcctcg ccgatgaact ggagcgtttt
     1681 tggtctcctt ttgcacatat gcatgcggtg atggattctg agacaatacg tgaatcttat
     1741 gaggcgtgtt tgccattgct gtctgcctat gatgctgcga ttgggcataa tcaagacttg
     1801 tatgaggcga ttaaatcgat agatcagcat tcccttaacc ctgcccagaa aaaaatcatt
     1861 gcagacagca tacaggattt cgagttatcg ggagttgctt tatccaaggc gaataaaaag
     1921 cgctttgaag ccattcaatc gagactggcc gaattatcca gcaaattcga aaacaatgtg
     1981 ctggacgcaa ctcatgccta cactattcat atcaccgaag cagaacgtat ggcgggatta
     2041 cctgagcatg cattaaatac ggcaaaagag ttggcgcatg agaaagggct cgatggtttt
     2101 gttttaaccc ttgaataccc ttgttttcag gccgttatta ctcatgccga agacagggct
     2161 ttacgtgaag aaatgtatcg agcttacatc accagagctt cagatcaagg ccctaatgca
     2221 ggaacatttg ataatactcc gctgattgat gaaattctgt ccctgcgcca tgaaaaagcc
     2281 gagcttttag ggtttaataa ttttgccgag ttatccctgg ctaccaagat ggctgcctcg
     2341 accaatcaag tcactgaatt tatctatgat ttaatcagca gaactcgtga caagggtaag
     2401 gctgaattta ggcaacttga agtgtttgca caggataaat ggaatctgag cccggtaaac
     2461 ccatgggacg ttgcttatct ttctgaaaaa agaagacagg atttgtattc cttatctcaa
     2521 gaggaattac ggccttattt cccacagcct aaagtgatgc aaggcttgtt cgcgattgtt
     2581 aaaaagctct ttggtatgag tatcgaggag attgagggcg tggatgtttg gcataaggat
     2641 gttcaatgct attgcatagt cgatgaatcc aatcaaacgc gcgggtacat ttatacggat
     2701 ttgtttgcca gaccgcacaa gcggaatggt gcctggatgg actcaatgca aagccgcaga
     2761 aaactggagg atggcacagt tcaattaccc attgcgacct taacttgcaa ttttgccaag
     2821 cc
//
LOCUS       DACTJE010000245         2741 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.247, whole genome shotgun sequence.
ACCESSION   DACTJE010000245 DACTJE010000000
VERSION     DACTJE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.247"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1026)
                     /locus_tag="I9760_002920"
     CDS             complement(<1..1026)
                     /locus_tag="I9760_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAT5044657.1"
                     /translation="MPKKDCILNLPGYSIKKVSGENPVYIEVNYRCVVRCIHCGNKKL
                     RVKDSFIRRIRHESIGLRRSYLCLKAHKYYCPSCGRYFNQRFPGIGKYQRASESLRKQ
                     VFHYHSKGVSQKDLARDLKLGKSTVERWYHYGYELRYKKIATRSCPRVLGLDEHSFNR
                     KVGYATTFCDLAKHKIFDVVEGRSEKDLESYLNTLEGKEKVQVVCIDLSSSYRKLIRH
                     YFPNAMIVADRFHVIRLLNQLCLQTYQQIDPEMKYQRGLLAALRTNPNNLTVKRLNRR
                     ERYLQQQPVIAAIYYFKQRLHRLLMRKHRTAKQCTRLIPLFLKLVASLKESPFESLKT
                     LGKTLYQW"
     gene            1265..1630
                     /locus_tag="I9760_002921"
     CDS             1265..1630
                     /locus_tag="I9760_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HAT5044658.1"
                     /translation="MVRFHLPAQKPYHTLDALEFSEKTFHLLGNFRSCKTKKEFGERI
                     ENLGGKIRGEGQTKNLNFKNTDYFVVGDNHYKEGQNGNKISRVDSYNSRHAPQEQIPK
                     ISESHCNELMEKYLQLKPK"
     gene            complement(1987..2352)
                     /locus_tag="I9760_002922"
     CDS             complement(1987..2352)
                     /locus_tag="I9760_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HAT5044659.1"
                     /translation="MKKSLTENITTSKQNPQRTNTRKHKVEFLAMREDISEALEKGWS
                     IIVIWETLRDEGSFTATYNTFRLYVLKYLNGQKSGYSQKDINVQQPKRLAVNQDKERK
                     PSAMPSFTFNPIPNLEELL"
     gene            complement(2537..2677)
                     /locus_tag="I9760_002923"
     CDS             complement(2537..2677)
                     /locus_tag="I9760_002923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044660.1"
                     /translation="MPIPSVFTLSTEGANKAREVWQPARWLANFTYLALHFEIYFGTV
                     FW"
BASE COUNT          851 a          550 c          480 g          860 t
ORIGIN      
        1 ccattgatat aatgttttac ctaacgtttt gagtgattca aaagggcttt ctttcagact
       61 ggcaaccagt ttaagaaaga gaggtattaa acgcgtacac tgttttgcgg tacgatgttt
      121 tcgcatgaga agccgatgta atcgctgttt aaagtaatat attgcagcaa tgacaggctg
      181 ctgttgcaaa taacgttctc gtctattgag tcgctttacc gtgagattat taggattagt
      241 tctcaatgcg gccaagaggc ctcgctgata cttcatttcg ggatcaatct gctgataagt
      301 ctgcaaacag agttgattca ataaacgaat cacatgaaag cgatcggcaa caatcatggc
      361 attgggaaag taatgcctga tcaacttgcg gtaactcgag cttaagtcaa tacagactac
      421 ctgtactttc tcttttcctt ccaaggtatt aaggtagctc tctaagtcct tttctgagcg
      481 cccctcaact acatcaaata ttttatgttt ggccaagtca caaaaggttg tagcataacc
      541 cacctttcta ttaaatgaat gctcatcaag tcctaatact cgggggcagg aacgagtcgc
      601 tatcttctta tagcgaagct cataaccata atgataccag cgctctaccg tcgatttacc
      661 cagctttaag tcgcgcgcca aatccttttg actgactcct ttgctgtgat agtgaaacac
      721 ctgtttacgc aaactctcgc tggctctttg atacttacct atccccggaa agcgttgatt
      781 aaaataacgt ccgcaagaag gacaataata tttatgcgcc tttaagcata aatagctacg
      841 acgtaaacca atagactcat gacgtatacg tcttataaaa ctgtctttta cgcgtaattt
      901 tttattacca caatgaatac aacgaacaac acaacgatag tttacttcaa tataaaccgg
      961 gttctctccg ctcacttttt taatagaata tcctggcaaa tttaggatac aatctttctt
     1021 aggcatttta atcttccttt tgaccgtcaa atcaaagggt tagtctagac taacttgatt
     1081 aaaatgcccc ctttgttggg gtagagccca agttttgaat attatatctt attcttatgg
     1141 gattatggga ttgaattgcc tcaattgatg tatcaaaatt gatgtatcaa aatcattggt
     1201 caagattaaa ccacaaagat acaagcatag actataatta agtaaagact ttcaaggacg
     1261 aattatggtc agattccatt taccagcaca aaaaccttat catacactcg atgcattaga
     1321 attctctgaa aaaacttttc atctcttagg caattttaga tcctgtaaaa ccaaaaagga
     1381 atttggagaa agaattgaaa atcttggcgg taaaataaga ggcgagggac aaaccaaaaa
     1441 tttaaatttt aaaaatactg attactttgt agtcggagat aatcactaca aagaaggtca
     1501 aaacggaaat aaaatctcta gggttgatag ttacaattca cgtcatgccc ctcaagaaca
     1561 aattcctaaa atatcagaat cacactgtaa tgaactcatg gagaaatatc tgcaactaaa
     1621 gcccaaataa ttctacgcaa atcaattaga taaatagaaa ttttacttct attgtaggtt
     1681 caaaaaatgc tgttacaaaa tacagcaagc gtagcctgta ttagacgaaa tcctaataca
     1741 ggatctattc catcaagcaa tccccttttc atgtaaatcc caatcaatgg gctcagactc
     1801 gattgataac atatataatt tataaacgta gattcacata agcatgttgt ccaatatctc
     1861 agggacaaag gggacatttg gggcaggcgc gttgctacta ggttttagcc gcccccaccc
     1921 catacaatga aggtggggca tgtgggactt attggaaact atatccacca ataaaaatcc
     1981 atccaattat aataattcct ctaaattagg tataggatta aaggtaaatg aaggcatagc
     2041 gcttggcttt ctctctttgt cttgatttac agccagtctc ttcggctgct gaacgttgat
     2101 gtccttttgc gaataaccgg acttttggcc attcaggtat ttcaatacgt ataacctgaa
     2161 tgtattgtaa gttgctgtga agctcccctc atccctaagc gtttcccata tgacgataat
     2221 tgaccatcct ttttccaagg cttcggaaat gtcttcacgc atagctagga attccacttt
     2281 atgttttctg gtgttcgttc tttgtggatt ctgtttagaa gtcgttatgt tttctgttaa
     2341 ggattttttc atggggtaca ctccatattt ttggttattc aatcctttga gttttatcgt
     2401 ttatttctat ttattttcga ccttttaggt cacatgcaac gccctttttg gtaactttcg
     2461 gtaatttatt atttatttct atttattcat gattattttc gttactaatc gttatataac
     2521 gaacaattac aaataatcac caaaagacgg ttccaaaata aatttcaaaa tgcagggcaa
     2581 gataggtgaa gttagctaac cagcgagctg gttgccaaac ttcacgagcc ttattcgcac
     2641 cttcggtgct caacgtaaaa accgatggta taggcaattt tattcacctc cccaaatctt
     2701 accggtaaaa acatataatc ttggggtgcc aactcccttt a
//
LOCUS       DACTJE010000246         2738 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.248, whole genome shotgun sequence.
ACCESSION   DACTJE010000246 DACTJE010000000
VERSION     DACTJE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.248"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            202..864
                     /gene="lem14"
                     /locus_tag="I9760_002924"
     CDS             202..864
                     /gene="lem14"
                     /locus_tag="I9760_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem14"
                     /protein_id="HAT5044661.1"
                     /translation="MSFELVAYEKLKGSIRESIITLIKSHNEKAKIIEDKLEYSVKEV
                     SRERQPQVLVLLKTIELLDNSSKEPEDKARVLNALAYYIRDQIAATYKYTSPDNSDFY
                     KSLTISLDLNKDNNPNREDLADMYSALEKFLRSHVYKNSDPRKGYLDKQPFAIKHYSV
                     VDDILELSDRVHKLRHEIIIAARDLHLLQQKPKSSQGGLFSVPSGKEKVTDTHEVANH
                     RL"
     gene            complement(888..1238)
                     /locus_tag="I9760_002925"
     CDS             complement(888..1238)
                     /locus_tag="I9760_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAT5044662.1"
                     /translation="MTKHKIKTIDVHELKHQMDNQANLSLIDVRELDEWEMMHIPGAL
                     HIPKDRISLEIQNQIPNKEQTIYLHCRSGVRSLYAAQCLMDLGYYEVYSVDGGIMAWA
                     MSGYPVKQESYTPS"
     gene            complement(1225..1506)
                     /locus_tag="I9760_002926"
     CDS             complement(1225..1506)
                     /locus_tag="I9760_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044663.1"
                     /translation="MLSELAKQFSTQIQTFFYLIMLINGLLHFIFAGAVARDAGNLYR
                     LGQKPVLVSAPTWAFATLIGGVITATIYWLLHHSTLTRPTMREIRYDKA"
     gene            complement(1510..2406)
                     /locus_tag="I9760_002927"
     CDS             complement(1510..2406)
                     /locus_tag="I9760_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAT5044664.1"
                     /translation="MLHFKTIQSILLLIVLGFIWGSGYTLAKFAMTNGVNPLGYAFWQ
                     SLGPALLLTLVSFFTKQTRLLHPVYWPYFFICGLIGIAIPNTNMYFIASHIPAGLLAV
                     LVNTVPLLVYPLALMAGQEKPDGWRFFALLLGMSGILAIIGINSAGLYSSWTILAMLS
                     PLGFAFCSIYIGAKQPKEINALQAANGMLLAASLLLTPVVLKQHAFYSLSAPLTLVKQ
                     IVILEIILSSLGYLLFFILIRMAGPVFYSLTGGVVAITGLFWGYLAFDEKPSSIQGMA
                     IALVVSAIFLLSWRQSKQTQGI"
BASE COUNT          937 a          484 c          523 g          794 t
ORIGIN      
        1 aaggcttaaa aatactctcc agaagtctat tcatttgatg aactaacaag gaaattacaa
       61 atttttcatt aaaatcaata gattgaaaat taattagata gtaaaaagaa aaagcatgac
      121 gaaaagagag aattggttta aactggccta cagtgaaagc atttcactag tcctgttcat
      181 ggagtataag gagaaaataa gatgtcattt gaattggtcg catatgaaaa attaaaagga
      241 agcatcaggg aaagtatcat tactttaatc aagtcccata atgagaaagc caaaattatt
      301 gaggacaaat tagagtactc agtaaaagaa gtttcaagag aaaggcaacc acaagtcctt
      361 gtcttgctaa aaactatcga actgctggat aactcatcca aagagccaga agataaggca
      421 cgcgtattaa atgcattggc ctattacatt cgtgatcaga ttgctgcaac ttataaatac
      481 acctctcccg acaacagtga tttttataaa tctcttacca tatcgcttga tttgaataag
      541 gataataatc ctaatcggga agatttggct gatatgtaca gtgctttgga aaaattcctt
      601 cgttcccatg tttataaaaa ttctgaccca agaaaagggt atttggataa acaacctttc
      661 gcaatcaagc attattccgt tgttgatgat attcttgaat tatctgatag agtgcataaa
      721 ttaagacatg agattattat cgccgctcgc gatttgcatt tactgcaaca aaaaccaaag
      781 agctctcaag gcggtctttt tagtgttcca tcaggaaaag aaaaagtcac cgatactcat
      841 gaggttgcaa atcatcgcct ttaagctttc tactctcacc tcttcactca ggaaggggtg
      901 taagactcct gttttaccgg ataaccactc attgcccaag ccataattcc accatccaca
      961 gaatagactt cgtaatatcc caaatccatt aagcattgag ctgcatacag tgatctgacg
     1021 ccgcttcggc aatgcaaata gatagtttgt tctttattgg gtatttggtt ttgtatttca
     1081 agagagatgc gatctttagg aatatgaagt gctcccggga tatgcatcat ttcccattcg
     1141 tccagttcac gtacatcaat caaactcaaa ttggcttgat tgtccatttg gtgttttagt
     1201 tcatgaacgt ctattgtttt gatcttatgc tttgtcataa cgaatttctc tcatagtggg
     1261 tctggttaat gtggagtgat gaagcagcca gtatatagtt gctgtgatga ctcctccaat
     1321 tagagtcgca aatgcccagg taggggccga tactaaaaca ggtttttgac ctagcctata
     1381 gagattacct gcgtctcgag ccactgctcc ggcaaaaata aaatgtaaaa gaccattaat
     1441 taacataatt aaatagaaaa aggtttggat ttgtgtgcta aattgttttg ctaattcact
     1501 gagcatgaat cagattcctt gagtctgttt cgattgtctc catgataaca gaaaaatggc
     1561 ggaaaccact aaagctattg ccatgccctg tatagatgaa ggcttctcat caaaagccaa
     1621 gtacccccaa aacaatccgg ttattgcaac gactccacca gttaaactat aaaaaacagg
     1681 tcctgccata cgaatgagaa taaagaaaag taaatatcct aaactggata aaattatttc
     1741 aaggataacg atttgtttca cgagagttaa cggagctgag agagaataaa atgcgtgctg
     1801 cttgaggaca acaggggtaa gcagaagaga cgccgccagt aacattccat tagcagcttg
     1861 cagggcgtta atttcttttg gctgtttggc tcctatataa attgagcaaa aagcaaagcc
     1921 aagagggcta agcatagcta atatagtcca gcttgagtaa agtcctgcgg agtttattcc
     1981 tataattgca agtatgccgg acataccaag taacaaggca aaaaatcgcc acccatccgg
     2041 tttttcttgg cccgccatga gagctaaagg gtaaaccagt aaaggaacag tattaactaa
     2101 cactgccaat aaacctgccg ggatatggct ggcaataaaa tacatgttgg tattaggaat
     2161 agcgatacct atcaatccac atataaaaaa ataaggccaa taaacagggt gtagtaagcg
     2221 agtttgtttt gtaaagaagc tcactaaagt taatagtagg gctggcccta atgattgcca
     2281 aaaagcatag cctagtgggt taactccatt agtcattgca aatttggcaa gagtatagcc
     2341 tgatccccag atgaatccta aaacaattag aagaaggatt gattgtatgg ttttaaaatg
     2401 gagcataaaa ggtaggataa atagtataat tttggataat tatgccattg aaagtagaaa
     2461 ataacgatat tcttgtgagg cctttaaatt ttgctcaata aaacagtttt ttaatcccta
     2521 caaatgctga attattattt tatatgattg cagtacattt tgccaaaaat cctggtcaag
     2581 aacatgaaga atcgatgatg gaataaatat tgctgtattc acaatatgtt gttgtgaaac
     2641 acgactatta tataaatgcc tctcttttct atatatgttg tcatcatgaa aactgtagta
     2701 ataatgtatt aagaatgata aatactttta ttttatga
//
LOCUS       DACTJE010000247         2722 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.249, whole genome shotgun sequence.
ACCESSION   DACTJE010000247 DACTJE010000000
VERSION     DACTJE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.249"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            175..855
                     /locus_tag="I9760_002928"
     CDS             175..855
                     /locus_tag="I9760_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HAT5044665.1"
                     /translation="MKPIAHIADKEIISIPVKECGEELIDLKNQNIIVYGPPPETPLT
                     ANDYTKIRKTVYQKLCLAQNDLPNGWRFRLYEGLRSLAVQEILFNQQRDNLRQSKEYD
                     SYEDLFNETCRLVSPVVHLDGTANIPPHSTGAAIDIELIDSYGNLINLGMAAKDWLIV
                     DPDLSETYSKLISEEAMQNRILMLKIMAKHGFVNYPREWWHFSYGDRLWAYMTEHSTA
                     IYGAIINN"
     gene            938..1666
                     /locus_tag="I9760_002929"
     CDS             938..1666
                     /locus_tag="I9760_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044666.1"
                     /translation="MTPSISLITSYSLLPAILMLIGGGIASLYRPGGSITSATQHFAA
                     GVVFAAVAKELLPKIGAYHDPLALIIGFSVGILAMLFLKWLANRLEDLEQEKTGLSWG
                     LLTAVGIDLLIDGILIGVAFLAGERGGILIAIALAIEIFFLGLSTTATLGARQVNVPI
                     RLLTSVILAILIPVGAALGASLLIHLPLSITNGILSFGVAALLYLVTEELLLEAHGVR
                     ETPYITLCFFIGFLLILLLEGLAA"
     gene            complement(1745..2713)
                     /locus_tag="I9760_002930"
     CDS             complement(1745..2713)
                     /locus_tag="I9760_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HAT5044667.1"
                     /translation="MKPIDLKEYLQKLKLGFQLLSDLPPEEQIKYLNSIYLAHIKVFP
                     YSNFELRRIANQHPVQRQSLSFFNYTKLLSSEHGGYCYQSAGLLFDALVQLGFKTEYC
                     AARVLVGEPVNSPKVLAIPATHLVLVVTVGDKKFLLDPGLGASAPRLPILITEKDESI
                     LQGKEEFKFYKQDGVHVLERKTSQGWFRLMQTDLIPIDLKTVELNLLKLERYPDTISI
                     RDNKTIVGSITEYGRKSLIWDAQSNQLKFSKHDGDEVIQKTLTSFEEGVQTLVKEFDI
                     DHISAQELKIYCTETNLPQPIKPWTVDLPLDQAELKRMEENLIFRL"
BASE COUNT          812 a          531 c          510 g          869 t
ORIGIN      
        1 gtttgagcca taattccctt aaatggctaa ttgcagattg ttaggtttta tcagaaaaat
       61 tttaaataat taaagcataa ggcctaacct gcttttttgt tagcatgata taattaatgt
      121 gcggataact tgtcgaatcc atgtaataaa attttgcttg ttttgcgaaa aataatgaaa
      181 cccatagcac atatcgcaga taaagaaata atatctatcc ctgttaaaga atgcggcgaa
      241 gagctcattg atcttaaaaa ccaaaatatt attgtttacg ggccccctcc agaaacccct
      301 ttaaccgcaa atgactacac caaaatacgt aaaaccgtat atcaaaaatt atgtttggca
      361 caaaatgacc tgcccaatgg ctggcgtttt agactatatg aaggattacg cagtctggct
      421 gttcaggaaa ttctgtttaa ccaacaacgt gataacctac gtcaaagcaa agagtacgat
      481 tcctatgagg atttatttaa tgaaacctgt cgtctggttt ctccagtagt ccatttggat
      541 gggacagcta atataccacc acatagcacc ggggccgcaa tcgacattga gctcattgat
      601 agctatggca atctgattaa tcttggaatg gctgcaaagg actggttaat tgttgatcct
      661 gatttaagtg aaacctattc aaaacttatt tctgaagaag ctatgcaaaa tcgtattctg
      721 atgctgaaaa taatggctaa gcatggtttt gttaattacc ccagagaatg gtggcatttt
      781 tcctatggag atcgcctatg ggcctatatg acagaacatt ctactgccat ctatggtgct
      841 atcataaata attaagccaa tgcggcaatg cctgccattc ttgaactggg ctaattattt
      901 gattattctg tcaatattac cttaaagaat atctcttatg accccatcta tttcgcttat
      961 tacctcctat tctttattac ccgccatatt gatgttaatc ggtgggggca tcgcaagcct
     1021 ttaccgtcca ggtggttcaa taaccagcgc aacccaacat tttgcagcag gtgttgtttt
     1081 tgctgctgta gcaaaagaac tgctacccaa aatcggagca taccatgatc ctcttgctct
     1141 tattatcggc ttttcagtag gcattctggc catgttattt ctaaaatggt tggctaacag
     1201 gctcgaagat cttgaacaag aaaaaacagg tctatcctgg ggattattaa ccgcagttgg
     1261 aattgattta ttaatagatg gtattttaat tggggttgct tttttggctg gtgaacgagg
     1321 aggaattctt atcgctattg cccttgccat tgaaattttc tttttgggat tatcgacgac
     1381 cgccacttta ggggcaagac aagtgaacgt gccaattcgt ctgttaacca gtgtcatttt
     1441 ggccatattg atccctgtgg gagctgctct gggcgccagt ttattaattc atttaccact
     1501 atcgattacc aatggaattt tatcatttgg tgttgcggca ttactttatc tggtcactga
     1561 ggagttattg cttgaagccc atggggttcg tgaaacccct tacattactt tatgtttttt
     1621 cattggattt ttgttgattt tattgttaga agggctggca gcataaatgc aagcaggtat
     1681 tttaataaaa acataagcta tgatgagctg aaccagcttg gaaaaacaaa tacaaatcac
     1741 ttttctatag tctaaaaatt aaattttctt ccattctttt taactctgct tgatcaaggg
     1801 ggaggtccac agtccaaggt tttattggct ggggaagatt cgtttcagta cagtaaattt
     1861 tcaactcttg cgctgaaata tgatcgatat caaactcctt aacaagtgtt tgcactccct
     1921 cctcaaaact ggttaaagtt ttttgaatga cttcatcacc atcatgtttt gaaaatttca
     1981 attgattcga ttgagcgtcc caaatcaacg atttacgtcc atattccgta atagatccaa
     2041 caatcgtttt attgtctcga atagaaattg tatccggata acgttccaat ttgagtaagt
     2101 tcagctctac cgttttgaga tctatgggga tcaaatcagt ctgcatcaag cgaaaccacc
     2161 cttgacttgt tttcctctcc agaacatgca caccatcctg cttataaaat ttaaactctt
     2221 ctttaccttg taaaatggac tcgtcttttt ctgttatcaa tattgggagc cttggagcag
     2281 aagcgcccaa tcccggatcc agaagaaatt ttttgtctcc cacagtcacg actaaaacaa
     2341 gatgagtagc aggtatcgca agtaccttag gggagttcac aggttcgcca acaagaacac
     2401 gcgccgcaca atattccgtc ttaaatccca attgcactaa tgcatcaaac aacagacccg
     2461 cactttggta acaatacccc ccatgctctg aagaaagcag ctttgtataa ttaaaaaaag
     2521 acaaggattg tctttggaca ggatgttgat tagcaattct gcgtaattca aaattggaat
     2581 aaggaaaaac tttaatatgt gctaaataaa tactgtttaa gtatttaatc tgttcttcgg
     2641 ggggtaaatc agataataat tgaaaaccta attttaattt ttggagatat tcctttaagt
     2701 cgatgggttt catataaaac ta
//
LOCUS       DACTJE010000248         2718 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.250, whole genome shotgun sequence.
ACCESSION   DACTJE010000248 DACTJE010000000
VERSION     DACTJE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.250"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            40..1137
                     /gene="lem23"
                     /locus_tag="I9760_002931"
     CDS             40..1137
                     /gene="lem23"
                     /locus_tag="I9760_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem23"
                     /protein_id="HAT5044668.1"
                     /translation="MKKKDEHNNLTKGKENEHNTISTNTHLLFSKPPGINADTANHMA
                     SSIDRLKDFDSSKQSLWLKYSEEIINEFCALLPEFPKKKPKVREDIRCPEEALLTQVG
                     CAYAFLSSKRREVAKLLKHGPLRHQFGLFNKPGFASIDDFNLSKDIPSQKDKIYEHLS
                     LLISQPNPFIEVKKNHREAEGSSIYTITFKMKPDEIEQVHKKNLDSNKPFIENDTVLT
                     ITFNDYGKNDNRTSIVIDHCPTPFAENYTSESSYFKVQFELIEPYFQACQAWEPSDGI
                     DHFLENAGRIVYILARMQPVGRGNSAIVEWMIRGLAQIKNIELGPFNPDEKIGWDFKA
                     FLTPEIQDYAHWFSEKAFAYSQLKNVTSLGQ"
     gene            complement(1332..1637)
                     /locus_tag="I9760_002932"
     CDS             complement(1332..1637)
                     /locus_tag="I9760_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2407 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044669.1"
                     /translation="MYNVVSFNNEGVEHMKPTSEKSKEIRKKYKAPGASDEQLANSIK
                     VASEFEYKNSSTFGWGGVYMVDRFQSMITGRAITNNTQKLLDAQALEEEASKANLKQ"
     gene            1860..2519
                     /locus_tag="I9760_002933"
     CDS             1860..2519
                     /locus_tag="I9760_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044670.1"
                     /translation="MLTKNLNEALSAIKYVKSKLVYGSNTEEDSKKPNWEPGRIILLR
                     KEVHALAVVYARSHNGKAGFDIISKLTHAALLKYGFGNCGEQAQTAFCYLQSRGVAPL
                     DYCQTSIGEHCLIVIGRVSGSDPNDISTWGEDAVICDPWAEKAYPLSEFRAMQSPDND
                     IRYPEICYANGKSPKPYLAGELISHHRIEPASKASSLSFFKKENYEHSQLNSATPMLI
                     A"
BASE COUNT          926 a          523 c          474 g          795 t
ORIGIN      
        1 gactatactt aagtaattca ttatagtttt tcgggtatta tgaaaaagaa agacgagcac
       61 aataacctta ccaaaggtaa ggagaatgaa cacaatacca tcagcaccaa tacccatttg
      121 ttattttcaa aaccacctgg tattaatgcc gatactgcaa atcatatggc ttcatctatt
      181 gaccgcttaa aagattttga cagctcaaaa caaagccttt ggttaaaata cagtgaagaa
      241 atcatcaacg aattctgtgc tttattacct gagtttccca agaaaaaacc caaagtacgt
      301 gaagacattc gctgcccgga agaggcatta ttaacccaag tcggttgtgc ctatgccttt
      361 ttatccagta aaagacgcga ggtagccaag cttttaaaac atgggcctct aagacatcaa
      421 tttggtttat ttaataagcc agggtttgct tcaatcgatg atttcaatct cagtaaagat
      481 atcccttctc aaaaggataa aatttatgag cacctctcct tgttaattag tcaacctaat
      541 ccttttattg aagttaagaa aaaccataga gaagcagaag gttcttctat ttacaccata
      601 acattcaaga tgaagcctga tgaaatagaa caagtacaca aaaaaaattt ggattcaaac
      661 aaaccattta ttgaaaatga cacggtactg acgataacct tcaatgatta tgggaaaaat
      721 gacaacagaa cctcaattgt cattgatcac tgccctactc cttttgctga aaactatacg
      781 agcgaatcca gctatttcaa agtgcaattt gaattgattg agccttactt tcaagcatgc
      841 caggcctggg aaccttctga tggaatcgat cattttttag aaaatgccgg aagaattgtt
      901 tatatactgg ctcgtatgca acctgttgga cgtggtaatt ctgcgattgt tgaatggatg
      961 attagaggcc ttgcccaaat caaaaacatc gaattaggac cattcaaccc tgatgaaaaa
     1021 attgggtggg attttaaagc gtttctgacg cctgaaattc aggattatgc tcattggttt
     1081 tccgagaaag cctttgcgta tagccaatta aaaaatgtta cttccctggg acaatgaatt
     1141 aagacattga attaaactga gtaaaccagc ctatgtggta aaaaaattta tagaattgta
     1201 ataaatagat caaagataat cccttaggat ataactgcag gtaagaagat aaaagagaac
     1261 gccgggattc aagtcttgct tttgactata agacacaaga aaaatgaaag acttgcccca
     1321 agataaaagc gttattgttt taagtttgct ttagaagctt cttcttcaag tgcttgagcg
     1381 tccaataact tttgtgtgtt gttagtgatt gcacgacctg tgatcattga ttgaaagcga
     1441 tcaaccatat atacaccacc ccaaccgaaa gtgctgctgt ttttatactc gaactcagaa
     1501 gctaccttga tagaattagc cagttgctca tcagaagccc ctggtgcttt atatttttta
     1561 cgaatttctt tacttttttc actggttggt ttcatgtgct caacaccttc attgttaaaa
     1621 ctgacgacat tatacatgaa taatattcaa acacaattta tttaaaaaaa acaatttgat
     1681 ctaaattttt cacaaacatt tctttagtaa aacactctcc attacatggc ctttacaatc
     1741 acttgagttt ccaaaaaata aaatctcaca atctaaaaac aaacccaaac agataaaata
     1801 ttaacaaatt gtattttatt tgatacaata actctgtttt actctctgag atttttttaa
     1861 tgctcaccaa aaatctgaat gaagccttaa gcgcaataaa atatgtaaaa tccaaactag
     1921 tttatggttc caataccgag gaagattcaa agaagccaaa ttgggagcca ggaagaatta
     1981 ttctcttaag gaaggaagtt catgcactag cagtcgtcta tgctcgttca cataatggca
     2041 aagcaggatt tgacatcatc tccaaattaa cccatgcggc actcttaaaa tacggattcg
     2101 gcaattgcgg tgagcaagca caaacagcat tttgttatct gcaatctcga ggagttgcgc
     2161 cgcttgatta ttgtcaaaca tcaataggag agcactgcct gatagtgatt gggcgcgtta
     2221 gtggttctga tcccaatgat atttcgacct ggggagagga tgctgtaatt tgtgatccct
     2281 gggctgagaa agcctatcct ctttctgaat ttagagcaat gcaaagtccc gataatgaca
     2341 ttcgctatcc tgagatatgt tatgctaatg gtaaaagtcc aaagccttat ttggcagggg
     2401 aattaatcag tcatcacaga atagagcccg cctcgaaagc atcatcgctc tctttcttca
     2461 aaaaagaaaa ttacgaacat tcacaattaa atagtgctac acccatgctt attgcttaaa
     2521 gcattccagt gccaaagtgc aatgactacc atgtgtacca attactatct tttgttgtaa
     2581 cgaatcatca aattatcctg gcgctattga ttaaaatctg gcaaagagaa aaaacacacc
     2641 ttgtgcgagc aaggaaaagc caggatatct gatgctctct tttacaaaat aattccaaaa
     2701 tcatccagta agaaaaat
//
LOCUS       DACTJE010000249         2711 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.251, whole genome shotgun sequence.
ACCESSION   DACTJE010000249 DACTJE010000000
VERSION     DACTJE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.251"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..364)
                     /locus_tag="I9760_002934"
     CDS             complement(<1..364)
                     /locus_tag="I9760_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044671.1"
                     /translation="MTLCPCGSQNKYEQCCGIYLESKKTPETPEQLMRSRYTAYSLAK
                     IDYIKQTMQGKPLIAFNESEAKQWAQNVSWLGLKIIQSYMERPDKGFVEFIATFLDGN
                     QLKSIHELSEFQKDNSVWF"
     gene            complement(626..1129)
                     /locus_tag="I9760_002935"
     CDS             complement(626..1129)
                     /locus_tag="I9760_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044672.1"
                     /translation="MPKKFTRKIPLISREECFALLPGYSINAMNLLKRGNHTPYYEEI
                     EYLSKIFVALVTHYAEPIESIINCMEKAMYGAYKEELDKIEHEIQRLFKIRKNTPIHS
                     LFGEREIEKLTIQISELEKRKSVPVKEIIQGVIYDTLDKASQTLVDRIPANYKLQEYE
                     SRDNPFQ"
     gene            complement(1236..2516)
                     /locus_tag="I9760_002936"
     CDS             complement(1236..2516)
                     /locus_tag="I9760_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HAT5044673.1"
                     /translation="MELTTTENQVFKSSKVSLALVTVLVGIGSGFLGMCLAMLLHYLQ
                     HIAYGYSPLHIISNETFLEGVRASSSERRISILFLCGLIAGMGWWALYRFGKPLVSIA
                     AAIKSGKPMPALSTFIHAFLQIITIALGSPLGREVAPREVSSVFAYWLSSKAGLTPEE
                     SKMMLACGAGAGLAAVYNVPLGGAVFVLEVLLCTSNWTVLLPALSSSAIAVLVSWWGL
                     GNESIYQLPEFSLSAPIILWSILASPVLGFFAFWFIQIATIQRSKAMHNWQMILACLI
                     NFLIIGLFAIYFPSLLGNGKSPAEMEFDQSVGIGLSIVLFMLRNVFVWSSLRVGAQGG
                     LLTPSLANGALLGAILGGIWNLYLPPAPIHAFALIGATAFLAAAQKMPLTAIILIFEF
                     TRINFIFLIPIMLAVSGSMGMCQLTKNYYIKYKV"
BASE COUNT          864 a          522 c          496 g          829 t
ORIGIN      
        1 aaaaccaaac ggaattatcc ttttgaaact cacttaattc atgaatactt ttcaattgat
       61 ttccatcaag aaaggttgct ataaattcaa caaatccttt atctggcctc tccatgtagc
      121 tttgaatgat tttcaaaccc agccaggaaa cattttgagc ccattgcttt gcttctgatt
      181 cgttgaaagc aattagtggt ttgccttgca tggtctgttt aatataatcg atttttgcaa
      241 gagaatacgc agtatatctc gaacgcatta attgttctgg tgtttcagga gttttcttgc
      301 tttccagata aatcccgcaa cattgctcat atttattttg tgaaccacag gggcaaagag
      361 tcatttagtt acctgtaaaa actggtttat gggtaaattt acaatccgtt atattaacga
      421 atcccggatt atattctata tggcattcca ttatttctaa acagttttgt tgcattggat
      481 ttttataaaa acagggacaa agtgtgattc attattaatc cacacagcga ttttaaaatg
      541 ggtaataaaa aaatgaccaa gtgctatcgg agtaaattag gttataagtg attggttgta
      601 tttcatttaa caatgtacct gaatcttatt gaaaaggatt gtctcggctc tcatattctt
      661 gcagtttata gtttgccggt atcctgtcca ccagagtttg gcttgcctta tctaaggtat
      721 cgtagataac tccctggata atttccttta caggtactga tttgcgtttt tccaattctg
      781 aaatctgtat agtcaatttt tcgatttctc tttcgccaaa tagagaatga attggagtat
      841 tttttcttat cttaaacagg cgttgaattt cgtgctcaat tttatctaat tcctctttat
      901 aagcaccata cattgctttt tccatgcaat tgatgattga ttctattggt tctgcataat
      961 gagtgaccag ggcaacaaaa atttttgata agtattcaat ttcttcataa tagggtgtgt
     1021 gatttcctcg tttcagaaga ttcattgcat tgatgctgta cccaggtaat aaagcgaaac
     1081 actcttccct ggagataagt ggtatttttc gagtaaattt cttaggcata gtgaacttcc
     1141 ttttcatcag gtctacatta taagtatagc atccaaagaa tttatattga tcaatatggt
     1201 tatatattgg gcaaattctg gctaattaca aacgatcata ctttatattt aatataatag
     1261 ttcttggtta actggcacat acccatagag ccggaaacag caagcatgat agggataaga
     1321 aagataaaat taatccgggt gaattcgaaa atgaggataa tggcagtcaa aggcattttt
     1381 tgtgccgcag caagaaaagc cgtggcacca ataagcgcga aggcatggat gggagcgggt
     1441 ggtaaataca aattccaaat tccacctaga atagctccta ataaggcgcc gttagccagt
     1501 gagggggtta gtaagccacc ttgtgctcct acacgcaagc ttgaccatac gaatacattc
     1561 ctcaacataa ataatacaat acttaaacca attcctactg attgatcaaa ctccatttct
     1621 gccggacttt tgccgttacc tagtaaagaa ggaaaataaa ttgcgaataa gccaatgatt
     1681 aaaaaattaa ttaaacaagc caatatcatt tgccagttat gcattgcttt gctgcgttgt
     1741 attgtagcaa tttgaataaa ccaaaaagca aaaaaaccta acaccgggct ggcaagaata
     1801 gaccaaagaa ttataggtgc acttaatgaa aattcaggga gttgataaat tgattcattt
     1861 cccaaacccc accaagacac tagaactgct atagcagaag aagataatgc aggaagcaag
     1921 acagtccagt tggaagtgca taacaacact tccaaaacaa aaacggcacc gcctaaaggc
     1981 acattataaa ccgcagcaag ccccgcgcct gctccacatg ccaacatcat cttgctttcc
     2041 tcaggagtta atcccgcttt gctagatagc cagtaagcaa agacagagct tacctcacga
     2101 ggagccacct ctcgtcccag aggtgagccc aatgcaattg ttatgatttg taaaaaagca
     2161 tgaatgaatg tactgagagc tggcatgggt ttacccgatt ttattgccgc cgcaatactg
     2221 actaatggtt taccaaaacg ataaagtgcc caccatccca tgcctgcgat taagccacaa
     2281 aggaatagta tgcttattct ccgctcagat gaagaggctc taactccctc cagaaaagtt
     2341 tcattactga tgatgtgaag ggggctgtaa ccataggcaa tatgttgaag ataatgcaat
     2401 aacatggcca ggcacatgcc tagaaaacct gaaccgattc ccaccagaac agtaaccaga
     2461 gcaagactta ctttggaact tttaaatact tggttctcgg ttgtggttaa ctccattccc
     2521 tggattcctt taaggtaact tgtcatcaat ttgcttatag gggtaagaat tttattattt
     2581 ctgtatgcag atttttttgc aagtgaaaca gattaataaa tgtacccgta ataacaaaat
     2641 ttaaaaaagt atccattgtc tgtttgcgat cttgacagca cattgcctaa aaagttaaca
     2701 ttaaacattc t
//
LOCUS       DACTJE010000250         2693 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.252, whole genome shotgun sequence.
ACCESSION   DACTJE010000250 DACTJE010000000
VERSION     DACTJE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.252"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..86
                     /locus_tag="I9760_002937"
     CDS             <1..86
                     /locus_tag="I9760_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="guanylate cyclase"
                     /protein_id="HAT5044674.1"
                     /translation="MTIVRENLPAKTGFKVRFVLTKGDPDE"
     gene            79..1356
                     /locus_tag="I9760_002938"
     CDS             79..1356
                     /locus_tag="I9760_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HAT5044675.1"
                     /translation="MSDYEVLLRHLDREIRARKEAEEISEKKISELYRFAQRLEESLK
                     AQKEISKKLKEKTKALKQQIAKTEEERHAKSTLQTILESAIEYSIIAINLKGVILVWN
                     EGARRNYGYEAQELVNKESIEILHTVEDIQSGKVRKFFQTVRREGKAEEIFQRVRKDG
                     SQFTASVTMTIRYDSQGKKVGYVIISKDITEIKEFEEKLIKSNQNLEEFSYIISHDLK
                     APLRAIIQLATWIEEDCGNKLDSLSRKNLQLLRERTRRMSGMVDGLLQYARAGRVNME
                     VEWVDTKELIQEIIDILNHDEQFTIIVHESMPQLPTAKLLLSQVFSNLIANSMKHHHC
                     KTGTIEIGVLDKGTFYEFFVADDGPGIEPVYFEKIFRIFQTLKSKDEAETTGIGLTIV
                     KKIVESQNGKIEVDSELGKGARFSFTWPKVPHQ"
     gene            1420..1827
                     /locus_tag="I9760_002939"
     CDS             1420..1827
                     /locus_tag="I9760_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT5044676.1"
                     /translation="MDLAADKVDILYLEDDEVDIQSVERVFHKISSLIKIEIAKSGNQ
                     ALDMLYGRNKENKIHPKLILLDINIPKMNGIEFLKELRDDSSFTDIEVFVLTAAYTSK
                     DKLAFESLNIRGHLIKPLDYGEAIKLFWILQSM"
     gene            complement(1839..>2693)
                     /gene="ankD"
                     /locus_tag="I9760_002940"
     CDS             complement(1839..>2693)
                     /gene="ankD"
                     /locus_tag="I9760_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkD/LegA15"
                     /protein_id="HAT5044677.1"
                     /translation="PSQFGHIAPTYQLMTDSVSHFAKSHPGNKNFQEIANAFQFSNEA
                     SAFKFSTPQRNPEAGNDLARRIQGGELTTIPVSCKGHAMGLSYVPDGPGSKSGYLVYT
                     NRGLGAKSSEHGTHIFRIEDSSKITPEFINNMTSGHSNGASHDEIMSQIKAAAGNKEP
                     IHHIKQKGQKNDNCTIANSKSNIEGILLCQKAREVGGFDKLTESDMDSVKKEYKEFTK
                     HMRVEKVNELAKALKENPQDPDLNNLTKEYLKQHPNADPKLKQTLETALKQASESSMT
                     LSQPGKTI"
BASE COUNT          854 a          439 c          584 g          816 t
ORIGIN      
        1 acatgactat tgttcgtgaa aatctgcctg caaaaacagg ctttaaggta agatttgtat
       61 taacaaaagg cgatcctgat gagtgattat gaagtattgt tacgccatct tgatagagag
      121 atacgcgcaa ggaaagaagc tgaagaaata agtgaaaaaa aaatatccga gttataccgt
      181 tttgcccaac gtctggaaga gagtttaaag gctcaaaaag aaataagtaa aaaattaaaa
      241 gagaagacca aggcattaaa gcagcaaatc gctaaaacag aagaagaacg gcatgccaaa
      301 agtactttac aaaccatatt ggaaagcgca attgaatatt caatcattgc aatcaatttg
      361 aaaggcgtca tcttggtttg gaatgaaggg gccagacgaa attacggcta cgaggctcaa
      421 gaattagtga acaaagagag cattgagatt ttgcatacag tggaagatat ccagtcaggt
      481 aaagtacgaa agttttttca aacagtacgt cgggaaggaa aggcggaaga aatatttcaa
      541 agagtcagaa aagacgggtc ccagttcact gcttccgtta ctatgactat ccgctatgat
      601 tcgcaaggaa aaaaagtagg gtacgtcatt atttcaaaag atattacaga aattaaagaa
      661 tttgaagaaa agctgattaa aagtaaccag aatcttgaag agttttcata cattatttcc
      721 catgacctaa aagcgccgct aagggcaatt attcaattgg caacgtggat tgaagaggat
      781 tgcggaaata agttggattc tctttccaga aaaaatcttc aattgctcag agaacgtacc
      841 cgtcgtatga gcgggatggt agatggctta ttacaatatg cgcgggcagg cagggtcaat
      901 atggaggtag aatgggtaga tactaaggag ttaattcaag aaataataga tattcttaac
      961 catgatgaac aatttactat tatagtccat gaatccatgc cccaactgcc aacggccaaa
     1021 ttacttctaa gtcaggtttt ttctaatctg atagcaaata gcatgaaaca tcatcattgt
     1081 aaaacaggaa caattgaaat tggtgttttg gataaaggca cattttatga gttttttgtg
     1141 gcagatgatg gtccaggtat tgagccggtg tattttgaga aaatattcag aatatttcaa
     1201 accttaaaat ccaaagatga ggcagaaact accggcatag gattaactat agtaaagaaa
     1261 attgtagaat ctcaaaatgg gaaaattgag gtggactctg agttgggtaa aggcgcaaga
     1321 ttttcattta catggcctaa agtccctcat caataaaggt ttaggtctat aattaattta
     1381 gaaataaaaa aataaaacat tgtctcacgg agaatagtga tggacctcgc agctgataaa
     1441 gtagatatcc tgtatttgga agatgatgag gttgacattc agtctgttga gcgcgtattc
     1501 cataaaatca gttcattaat caagattgaa atagctaaaa gtgggaatca ggcattggat
     1561 atgctatatg gtcgaaataa agaaaataaa attcatccca agttaattct tctcgacatc
     1621 aatataccta aaatgaacgg tattgagttc ttaaaagaat taagagacga ctcttccttt
     1681 acggatatag aggtatttgt acttacggca gcctatacca gcaaggataa gctggctttt
     1741 gagtctttaa atattcgagg ccatttgatt aaaccactcg attatggcga ggctattaaa
     1801 ctattttgga tattacaatc catgtaggct cggtagtatt aaatggtttt ccctggttgg
     1861 cttagtgtca tactgctttc agaagcttgt tttaatgcag tttccaaggt ttgttttagt
     1921 ttagggtcgg cgtttggatg ttgcttcaaa tattctttgg ttaaattatt taaatcagga
     1981 tcctgaggat tttctttaag ggctttggct aattcattga ctttttcaac tctcatatgt
     2041 tttgtaaatt ccttgtattc ttttttaacg gagtccatat cgctttctgt taatttatcg
     2101 aaccctccca cttctctggc tttttggcat aatagtattc cctctatgtt ggatttggag
     2161 ttggctattg tgcaattgtc atttttttgt cctttttgtt tgatatggtg aatgggctct
     2221 ttgtttccgg cagctgcttt aatttgagac ataatttcat catgagacgc gccattcgaa
     2281 tgtccacttg tcatattatt gataaattca ggtgtaattt tgctggaatc ctcgattcgg
     2341 aaaatatggg tgccatgctc gctgctcttt gctccaagac cacgattggt atataccagg
     2401 tagcccgatt tcgaaccagg gccgtcgggt acatacgata atcccatggc atgaccttta
     2461 cagctgacag gaattgtggt gagttcgcct ccttgaatgc gtcttgcaag atcgttacct
     2521 gcttccggat ttctttgtgg tgtgctgaat ttgaatgccg atgcctcatt tgaaaactgg
     2581 aaggcattgg ctatttcctg aaagttcttg ttgccaggat gagatttggc aaaatgactg
     2641 accgaatctg tcatcaattg ataagtgggc gctatatggc cgaattgaga ggg
//
LOCUS       DACTJE010000251         2677 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.253, whole genome shotgun sequence.
ACCESSION   DACTJE010000251 DACTJE010000000
VERSION     DACTJE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.253"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1482
                     /locus_tag="I9760_002941"
     CDS             <1..1482
                     /locus_tag="I9760_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HAT5044678.1"
                     /translation="AKFQNFLEENIYSCPENITFQTLARIKEICPECELGEKLFITGT
                     NLSTQKHEVFSIDTTPSMALADAIIISANLPIAFERICYQGNVYSDGGISNNLPAHCF
                     SEKGHKTTFLKHKDDVDFSVLALQFDNGLEENALYSQNPIPKWSWLSNTFYSLITGHP
                     NVTENWHEDLQILRRHAHQSILIKTPTIALTNLTISQDTKKALVESGRTAAKTYLELH
                     EFYTDDYGNIRHNECLHEKFQKPEELLDYCVLHSHFALLKKIKQAISCSQYLEKGYKH
                     YLCELCDNLLPPQLKCPNEGSGTEQPEIKLEKDTIICKKNNNSGLTFSMTFFGVPSPL
                     GKTLNQDSPELKIKLFTGLYPILIQNWQNLCPVSGISGILNSIRMSFVEISSTDTCIK
                     TLIDKLNEIEIGHFLIFVFKAALKNYDKHDFILLLKNLKHLHHSIELIRNKPFHSDDR
                     FYGQWSFEGHDPKRILEFIKSDDISGLMTILEDKKALPNNKPN"
     gene            complement(1585..2412)
                     /gene="lem24"
                     /locus_tag="I9760_002942"
     CDS             complement(1585..2412)
                     /gene="lem24"
                     /locus_tag="I9760_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem24"
                     /protein_id="HAT5044679.1"
                     /translation="MWIFKDITDRYYKKILDSYQLQADEKTYKGKEAELLIQRNELAR
                     CLIKTISLMEVKTQEDYANYFKRVSDEINSALQAAKNAVKEYNEEHNTDFTTDLYESF
                     FTYSLSNFIEVVSDLFQKSPSIITNIMDESLFCNSRNVPWVYQLSFVFCDYILEKEFE
                     FITTKASRDIFDAKKQLVLKYIQKAADLYPRYEKEDFSDCHEMVNILLLSMRSEENNL
                     QKRRNQDSSTSYMYSYFTQTLYNASGKLMGKGQLGQKIDLLIEEFNERAMNKGPSYT"
BASE COUNT          917 a          476 c          422 g          862 t
ORIGIN      
        1 gcaaaatttc aaaatttttt agaagaaaat atatacagct gccccgagaa tataacattt
       61 caaaccttag ccagaataaa agaaatatgt cctgaatgtg aattaggaga aaaactattt
      121 attactggaa caaatttgag tacccaaaaa catgaagtgt tttctattga tacaacacca
      181 tctatggcac ttgccgatgc cattattatt tccgcaaatt tacctattgc ctttgagcgt
      241 atctgctatc aaggaaacgt atacagcgat ggaggaatca gcaataattt acctgcccat
      301 tgtttttcag aaaaagggca taaaacaacc tttttgaaac acaaagatga tgttgacttc
      361 agtgtacttg ctctgcagtt tgataatggg cttgaagaga acgctcttta tagccagaac
      421 ccaataccta aatggtcatg gctaagcaac acattctact cattgattac aggacatcca
      481 aatgttactg aaaactggca tgaggatctt caaatactga ggcgacatgc ccatcaaagt
      541 attttaatta aaacgccaac tattgctttg accaatctca caattagtca agatacaaaa
      601 aaagccctgg tcgaatcagg acgaacagct gccaagacct atctggaact ccatgagttt
      661 tatacggatg attatggaaa tatcagacat aatgaatgct tgcatgaaaa atttcaaaaa
      721 cccgaagagt tattggatta ttgtgtccta cacagccact ttgcactttt aaaaaaaata
      781 aagcaagcca tttcctgctc acaatattta gaaaaagggt ataaacatta tttatgtgag
      841 ttgtgtgaca acttgcttcc acctcaactg aaatgcccca acgaaggttc aggaacagaa
      901 caacctgaaa tcaagttaga aaaagatacc atcatttgca aaaagaataa taatagtggg
      961 ctgacatttt caatgacatt ttttggggtt ccatcccctt taggcaaaac attaaatcag
     1021 gatagcccag aattaaaaat taagctattt acaggtttat atcctatact aattcaaaac
     1081 tggcaaaatt tatgccctgt ttcaggcatt agtggtatat taaattctat ccgcatgtca
     1141 ttcgttgaaa tcagttcaac agatacctgt ataaaaacat taattgataa actgaacgaa
     1201 attgaaattg gacatttttt gatatttgtt tttaaagcag ctttaaaaaa ttacgataaa
     1261 catgacttca tattactatt gaaaaacctg aagcatcttc atcattctat cgagttaatt
     1321 agaaataaac cgtttcattc tgacgaccga ttttatggac agtggagttt tgaagggcat
     1381 gatcccaaaa gaatcctcga attcattaaa agcgatgata tttcaggatt aatgactatt
     1441 ttagaagata aaaaggcgct gcccaataat aaaccgaatt aacaggttta aatccaaagc
     1501 gtcactgagt taacaaatca acttgaattg gtgtttttac tattaatcaa gtgatctttc
     1561 tgccttgttt tggaaaataa acaattaagt atacgatggt cccttgttca ttgctctttc
     1621 attaaattcc tctatcaata aatctatttt ttgccctaat tgccccttcc ccattaattt
     1681 cccggaagca ttataaagtg tttgtgtaaa ataggagtac atataacttg tgctactatc
     1741 ctgatttctg cgcttttgta aattattttc ttcagaacgc atgctaagta acaggatatt
     1801 taccatctca tggcaatcag agaaatcctc tttttcatag cgaggataaa gatcagccgc
     1861 cttctgtata tatttaagaa ccaattgttt ttttgcatca aaaatatctc tgctggcttt
     1921 agtggttata aattcgaatt ccttctcaag gatatagtca cagaatacaa aggacagctg
     1981 atacacccag ggtacatttc tactattgca aaacaatgac tcatccatga tattagtaat
     2041 aatagaagga cttttttgaa acaggtcaga tacgacttca ataaaattgc ttaaactgta
     2101 tgtaaaaaaa ctttcataca aatctgttgt gaaatcagta ttatgttctt cattatattc
     2161 tttgacagca tttttggctg cttgtaatgc cgagttaatt tcatcagaga ctcgtttaaa
     2221 ataattagca taatcctctt gggttttaac ttccattaat gaaatggtct taatcaaaca
     2281 tctggcaagc tcatttcgtt gaattaataa ttccgcttct ttgcctttat atgttttttc
     2341 atctgcctgc aattggtagc tatccaaaat ttttttataa tagcggtcgg taatatcttt
     2401 aaaaatccac attatctacc tctttaatta gtgatccaga aagtatttgc aaaaattgtc
     2461 gctgattatc aagaacaata acgtcttcct tttctattac gggcgatagt caatattgta
     2521 atgacagcaa acacatgatt tccttgtctg atgtattggt aatccattga taactgccaa
     2581 gaattataaa taaatgttca aaaaacagta ggaatagcca tgatggctaa tagtaactca
     2641 ttgtaagtta taggaaaagt atctgtattc ggttttt
//
LOCUS       DACTJE010000252         2542 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.254, whole genome shotgun sequence.
ACCESSION   DACTJE010000252 DACTJE010000000
VERSION     DACTJE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.254"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1465)
                     /locus_tag="I9760_002943"
     CDS             complement(<1..1465)
                     /locus_tag="I9760_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAT5044680.1"
                     /translation="MTKSRKLKRNLLEISKTEAGQYSVSAPEHKGLVLSGGGAKGISY
                     LGMIQALQERGKIKNLTHVSGASAGAMTASILAVGMDIKDIKKLIEGLDITKLLDNSG
                     VGFRARGDRFRNILDVIYMMQMKKHLESVQQPIPPEQQMNYGILKQKIALYEDKLSRA
                     GIVINNVDDIINLTKSVKDLEKLDKALNSIPTELKGAKGEQLENPRLTLGDLGRLREL
                     LPEENKHLIKNLSVVVTNQTKHELERYSEDTTPQQSIAQVVQWSGAHPVLFVPGRNAK
                     GEYIADGGILDNMPEIEGLDREEVLCVKAEAGTAFEDRVNKAKQSAMEAISWFKARMD
                     SLVEATIGGKWLHATSSVLNREKVYYNIDNMIYINTGEVTTTNTSPTPEQRARAVKNG
                     YDQTMQLLDSHKQTFDHPLMAILYIGHDKLKDALIDEKSEKEIFEASAHAQAILHLQE
                     QIVKEMNDGDYSSVQNYLDQIEDILTVDAKMDDIQKEK"
     gene            1753..2475
                     /locus_tag="I9760_002944"
     CDS             1753..2475
                     /locus_tag="I9760_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAT5044681.1"
                     /translation="MATRNPFDIDHKSKYLREAALEANLSHPETTPTMLTCPIDSGFL
                     KDPVITPEGFVYNKSSILKWLETKKEDPQSRKPLTAKDLQPFPELLIIVNRFVETQTN
                     YEKLKNRLVQNARVAARQKEYTEIPDIFLCPISKTLIKTPVITAQGKVYDQEALSNFL
                     IATGNKDETGKKLSIDDVVVFDELYQQIKVYNFYRKREMQKNQIQPSVSSGFGFFSLN
                     FLTSWLWGTEEKKEKTSSDMTY"
BASE COUNT          754 a          509 c          447 g          832 t
ORIGIN      
        1 ccttctcttt ctggatgtca tccattttgg catcgactgt cagaatgtct tcaatttgat
       61 ctaaataatt ttgcacgcta ctgtaatcac catcattcat ttctttgacg atttgttctt
      121 gaagatgcaa aatagcttgt gcatgcgctg atgcctcaaa aatctctttc tctgatttct
      181 catcaatcaa tgcatccttg agtttatcat gtccgatata aagtattgcc attaatggat
      241 gatcaaatgt ttgcttgtga gaatccagta attgcattgt ctgatcgtag ccatttttta
      301 cagcgcgcgc tctttgttca ggtgttggag aggtgtttgt ggttgtgact tcccctgtat
      361 taatgtaaat catattatca atgttataat agactttttc acgatttaac acggatgaag
      421 tggcatgaag ccattttcca cctattgtag cctcaaccaa agaatccatg cgtgccttaa
      481 accagcttat cgcttccata gcactttgct tggccttatt aacgcgatct tcaaatgcgg
      541 tgcctgcctc tgctttgaca caaagcacct cttctctgtc aagcccttca atttccggca
      601 tattgtccag aattcctcca tcagcgatgt attcaccctt ggcatttctg ccaggtacga
      661 aaagtaccgg atgggcccca gaccactgga caacttgtgc tatagattgt tgaggagttg
      721 tgtcttcact gtagcgttcc agctcatgct ttgtttgatt agttacaact acagacaagt
      781 ttttaattag atgtttattt tcctcaggca gcaactctct taagcggccc aagtcaccaa
      841 gagttagacg aggattttca agttgttctc ctttggcgcc tttcaactca gtaggaatac
      901 tattaagtgc tttatcaagt ttttccaaat cttttactga ttttgtcaag ttgataatat
      961 cgtctacatt atttatcaca atccctgctc ttgataactt gtcttcatag agagctattt
     1021 tttgtttaag tatgccataa ttcatctgtt gttctggagg aatgggttgc tgcacgcttt
     1081 ctaaatgttt tttcatttgc atcatgtaaa tgacatccag aatgttacga aaacgatctc
     1141 ctctcgcacg aaaacctact ccgctattat ctaatagctt ggtgatgtcc aatccctcaa
     1201 tcagtttttt gatgtctttg atatccatgc caacggctaa tatactcgcc gtcattgctc
     1261 cggcagatgc tccgctaaca tgggtcagat tttttatttt tccgcgctct tgtaaggctt
     1321 gaatcatacc caaataggat atgcctttcg ctccgccgcc acttagcacc aaccctttgt
     1381 gctcaggcgc actaacagaa tattgtcctg cctcagtctt tgatatttcc agtaaattcc
     1441 tttttaattt acggcttttt gtcataattt cagcaagttt caaacagaat ttaatttctt
     1501 atagttttat tatagtagtt aattttgact ttgtgatttt taaacaaaca attatgtgag
     1561 caattgattg ggggaccttc ctcgaaccac tataagcatg tagtcttttt tactggcagc
     1621 ttgagatacg gtgctcttta ttagcgaaag cacaaataat gaaggagtga ttcacctata
     1681 aaacaaataa attgcataaa aataaatcta atggtaaaat attgcatcat ttagttgggg
     1741 tatagaatag aaatggcgac acgaaatcct tttgatattg atcataaaag taaatactta
     1801 agagaagcag cattagaggc caatttatct catccagaaa caacaccaac aatgctgact
     1861 tgccctattg acagcggatt tctaaaagat cccgtgatca caccagaagg ttttgtttat
     1921 aataaatcct ctattttaaa atggttagaa acgaaaaaag aagacccaca aagccgtaaa
     1981 cccttaacgg ctaaagattt gcaaccattc cccgagttat tgattatagt caatagattt
     2041 gttgagacac aaacgaacta tgaaaaatta aaaaacagat tagtgcaaaa tgctcgggtt
     2101 gctgcacgcc aaaaagaata cactgaaatt ccggatatat ttctttgccc aataagtaaa
     2161 acgcttatca aaacacctgt cattactgcc caagggaaag tatatgatca agaagcatta
     2221 agtaactttc ttatcgcaac gggtaataaa gatgaaacag gcaaaaaatt atccattgat
     2281 gatgtagtgg tgtttgatga actctatcaa cagataaaag tttataattt ttaccgcaaa
     2341 cgcgaaatgc aaaaaaatca aattcaacct tcagtaagta gtggttttgg cttttttagc
     2401 ttgaattttc tcacctcatg gttatgggga actgaggaga aaaaagaaaa gacatcatct
     2461 gatatgacgt actaatgccc cgtttttgcg caatattgat tcaaagtgaa gtaaattttg
     2521 agagaaaaaa ccactatact gg
//
LOCUS       DACTJE010000253         2513 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.255, whole genome shotgun sequence.
ACCESSION   DACTJE010000253 DACTJE010000000
VERSION     DACTJE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.255"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..765
                     /gene="legC6"
                     /locus_tag="I9760_002945"
     CDS             <1..765
                     /gene="legC6"
                     /locus_tag="I9760_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC6"
                     /protein_id="HAT5044682.1"
                     /translation="KTSEIIRDLNRSKEQNGIEPAEVMKLFQIIAVIFETRNILINWI
                     TAPVHLKEQTEEALLGAGKTPAIVEGKGTQPKRVRFLPDLVEKPKATSPDTEQPVTKR
                     KDDELLDKKIQVALEKVDQALEELKGKIGDINQHKYDKAYEAAKILLRQLQETRDQYA
                     IDLANPQINFKEAGLKFKEASNLVIQQAKPILEKDLGWGEYLKNLFKVIVNAIVFCVT
                     FGASQGFFATTRAKSAEAVEKAESELELNQLGSLPK"
     gene            complement(873..1430)
                     /locus_tag="I9760_002946"
     CDS             complement(873..1430)
                     /locus_tag="I9760_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAT5044683.1"
                     /translation="MARTLSNMLPLGTVAPHFELLDVVSGSKVKLRETPRYVATVIMF
                     ICNHCPFVKHINNELTQLANDYIPQGIRFLAINSNDVESYPDDSPENMIQTAKVHQYP
                     FPYLFDETQEVAKAYNAACTPDFFVFDSDLSLVYRGQLDDSRPGNGIPVTGNSIRQAL
                     DCLLNNKPFDFEQKPSIGCNIKWKI"
     gene            complement(1454..1954)
                     /locus_tag="I9760_002947"
     CDS             complement(1454..1954)
                     /locus_tag="I9760_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044684.1"
                     /translation="MSKTKHDTLYLVLGIVGALLLIYGLSQNNPQVYYITGSLALMIT
                     AIHYKLIYFIALELILGAGHTSLLLGMGRYIGLALPSLLCLQLLIFYLMFGKGNSIFL
                     LTGIIGIALLSMGLAYHNQIIFFSGSSGVAAYSYYSAYRGQYICYIWGILNTIFASYA
                     LYQLVF"
     gene            complement(1958..2446)
                     /locus_tag="I9760_002948"
     CDS             complement(1958..2446)
                     /locus_tag="I9760_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced entry protein EnhB"
                     /protein_id="HAT5044685.1"
                     /translation="MSLINYFTKTLLITAFATNAVQAASIFPRGCEVTGFGYSENYLI
                     LNETGNQTYYLIQNRSDSKIELERFDNSDAFMIPPLQASLDPMSWAAFASDVKNLNFK
                     CYKHVDENTALVDCRDVLEVCQYPRVKFALSNMGNYWISTNKSQNEVIQESVAKGIYL
                     KW"
BASE COUNT          907 a          430 c          448 g          728 t
ORIGIN      
        1 aaaacttcag aaataattag agatcttaat cgttcaaaag agcaaaatgg tatagagcca
       61 gctgaagtaa tgaaattatt tcaaataatt gcagtaatat ttgaaactcg aaatatttta
      121 atcaattgga taactgctcc tgtgcatttg aaggagcaaa cggaggaagc tttattgggc
      181 gcaggtaaaa ctcctgccat cgttgagggt aaaggaaccc aacccaaacg cgtaagattt
      241 ctacctgatt tagtggaaaa gccaaaagct acatcgccag atactgaaca acctgttacc
      301 aaaagaaaag atgatgaact acttgataaa aaaatacaag ttgcattaga aaaggttgat
      361 caagcattag aagaactgaa aggtaaaata ggggatatca atcaacataa atatgacaaa
      421 gcctatgaag cagcaaaaat tcttctaaga caattgcaag aaacacgtga tcaatatgct
      481 attgatttag ctaatcccca aatcaatttc aaagaggcag ggcttaaatt taaagaagct
      541 agtaacctgg tgattcaaca ggccaaacct atcctggaaa aggatttagg ttggggagaa
      601 tacctcaaga acttgtttaa agtgatagta aacgctattg tattttgtgt gacttttgga
      661 gcaagtcaag gcttctttgc tacgacacgt gcgaaatctg ctgaagcagt ggagaaagcg
      721 gagagcgagc tggaactaaa tcaactcggt tcattaccaa aataatacat atggttaact
      781 agtttgttat gagagcaaca ttcgattttt attatacata taatgagtaa tggaaatcga
      841 tgttgctttt ttgaattaaa gatagattca gcctatattt tccacttgat gttgcatcca
      901 atacttggtt tttgttcaaa atcaaaaggt ttattattta ataagcaatc caaagcctgg
      961 cgtattgagt tgccagttac aggaatacca tttcctggtc ttgaatcatc taattgccct
     1021 cgataaacta aagacaaatc agaatcaaaa acaaagaaat ctggtgtgca agctgcatta
     1081 tatgcttttg ctacttcctg ggtttcatca aaaagatatg gaaatggata ctgatgaact
     1141 ttagcagttt gaatcatatt ttcaggtgag tcatcagggt aactctctac atcattggag
     1201 ttaatagcta aaaaacgaat tccttgggga atatagtcat tagcaagttg tgttaattca
     1261 ttattgatat gtttcacaaa gggacaatgg ttgcatataa acataatcac agtagccaca
     1321 tagcgtggag tctctcttaa tttaactttg cttccactaa ctacatccaa taattcaaaa
     1381 tgaggagcta ctgtacctaa tggaagcatg tttgatagag ttctagccat tttattaaat
     1441 ccctcaaata atcttaaaat accaattgat aaagagcata tgacgcaaat attgtattaa
     1501 gaatgcccca tatgtaacag atgtattgac ctctatacgc actataataa gaatatgcag
     1561 ctacgcctga acttccagaa aaaaatatga tttgattatg gtaggcaagc cccatcgaaa
     1621 gcagtgctat tcctataatg ccggttaata aaaaaatact gtttcccttg ccaaacatta
     1681 aataaaaaat cagtagctgt aaacacagta aagaaggtaa agctaatcca atatatctcc
     1741 ccataccaag gagtagggag gtatggcctg cccccaaaat taattccaga gcaataaaat
     1801 aaatcaattt ataatgaatt gctgtaatca tgagagcaag tgagccagta atgtaataga
     1861 cttggggatt attctggctc agcccataaa taagtaacaa agcaccaaca atacctagta
     1921 ccagatatag cgtgtcatgc tttgttttac tcacggatca ccattttagg taaattcctt
     1981 tagcaaccga ttcctgtata acttcatttt gtgatttatt agtagaaatc caatagtttc
     2041 ccatattact aagtgcaaat ttaacacgag gatattggca gacttcgaga acatctcgac
     2101 aatcaactaa agcagtattt tcatcaacat gcttataaca cttaaagttt aaatttttta
     2161 catctgaggc gaatgctgcc caactcattg gatctagcga tgcttgtaag ggaggaatca
     2221 taaacgcatc tgagttatca aaacgttcaa gctcaatttt cgaatctgaa cggttttgaa
     2281 tgagataata ggtttgatta cctgtttcat ttaaaattaa ataattttca ctataaccaa
     2341 aaccagtgac ttcacagcca cggggaaaaa tcgaagcagc ctggactgca ttggtcgcaa
     2401 aagctgtgat taacagcgtt ttagtaaaat aattaattaa tgacataatt tcctcagaaa
     2461 acaaaattaa gatgctattt taatcattaa atagacaaag tctatcatgg aat
//
LOCUS       DACTJE010000254         2452 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.256, whole genome shotgun sequence.
ACCESSION   DACTJE010000254 DACTJE010000000
VERSION     DACTJE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.256"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            41..1564
                     /gene="ankQ"
                     /locus_tag="I9760_002949"
     CDS             41..1564
                     /gene="ankQ"
                     /locus_tag="I9760_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkQ/LegA10"
                     /protein_id="HAT5044686.1"
                     /translation="MLMAATIPHIETITFTDWFLAIIDTPDIPIDEIEIRVAEGFKRK
                     LTERQFQQLTELIHLISFIKHKKISNPTLALRIYVDENTSSLSRTALVEAVRMLPPED
                     NKTYELAAYLAQKNKLERYTNITKVPPLQIYQGDGVFEQYDEWILLFELFGLTQATPS
                     DFIPAIAHLLITKNLGIPDLRALSKFMSTTHKLGILSQSFMNNMTPHLCNGQIIEKYE
                     SLLLKLQINDLLTEEILEVIYPLLNQPDALDAFFSLYHEEPSHDSALSFLREILPSFC
                     AMSLPSKESYDDQVPTNTPLHLAVIESDKGNLERTLAFANRNLLIKCSYENTALLLAC
                     KLADKESARLILAKMQELGCDVNQRDHHGMTALHWANFYHFDELIRELEIAGADPQLK
                     AANGKTCEYFYQHQFTLKDLKLNGKEIIDGEFKLSDCALTDIAFHMDKIALNLKLTTP
                     SEIAELYARDEIAQIRSSNRFYLFFKLFRPKLINWLEKQNDLEPQVTYRFSGQSSSA"
     gene            1725..>2452
                     /locus_tag="I9760_002950"
     CDS             1725..>2452
                     /locus_tag="I9760_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAT5044687.1"
                     /translation="MVRLRIYLCSLLLVIPFYRAYALNLTIGTLSYNPPFETQVNDSS
                     KKNEFYGFEIELMDEICERINAKCQYKPLLFEQILAEINSGAINLGIGSINITPERSE
                     RFLFSLPYKASYHQFLIPSNSNIKKIDDLKGKTIGIHLGSPTADYLKAYFNNNIQFKY
                     YETFMDLLNGLTDPQVSAIVTNSDQAQYWASSTPDCKLLGDSFPFGVGFGVVARLDQA
                     ELIEKINQALIEIENDGTYLKIYNL"
BASE COUNT          814 a          458 c          433 g          747 t
ORIGIN      
        1 taaagctttt tgtttataat attgacaatt tatttaagga atgcttatgg ccgcaacaat
       61 tccccatatt gaaacaatta cttttaccga ctggttcctg gcaatcattg acactccaga
      121 cattcccatc gatgaaattg aaatcagagt agctgaaggc tttaagagaa aattgacaga
      181 acggcaattt caacaattaa cggaattaat ccatttaatt agtttcatta agcacaagaa
      241 aatctccaat cctaccttgg ctctccgcat ctatgttgat gaaaatacga gctctctatc
      301 aagaactgct ttggttgaag cagtcaggat gcttccacct gaagataata aaacctatga
      361 gttagcagcc tatttggctc aaaaaaacaa actggaacga tatactaaca taacgaaagt
      421 gcctcctctg caaatttacc agggggatgg cgtatttgag caatacgatg aatggattct
      481 tctttttgaa ctgtttgggt taacccaggc aacgcccagc gatttcattc ctgccattgc
      541 tcatttatta atcaccaaga atcttggtat tcctgatctt agggcacttt caaaatttat
      601 gagcactacg cataaactag gcatattaag ccaaagtttt atgaataata tgactcccca
      661 tttatgtaat gggcaaatca tagaaaagta tgagtcatta ttgcttaaat tacaaattaa
      721 cgatttgtta actgaggaaa ttctggaagt gatctatcca ctcctcaatc aaccagatgc
      781 tcttgacgct tttttttcac tctatcatga agaaccatcg cacgacagcg ctctgtcttt
      841 cttgagagaa attttgccat ccttttgcgc aatgagcctc cccagtaaag aaagctatga
      901 cgatcaggta ccaacaaaca cgcctttgca tctggccgtt attgagagtg ataaagggaa
      961 tctggaacgg acattagcct ttgccaatcg caatttacta ataaaatgct cttatgaaaa
     1021 tacagcactt ttattagctt gtaaacttgc tgataaagag tcagcccgat tgattttagc
     1081 gaaaatgcag gaattaggct gtgatgtcaa tcaaagagat catcatggca tgaccgcgct
     1141 gcactgggct aatttttatc attttgatga gctaatcagg gagctagaga ttgctggggc
     1201 ggatccccaa ttaaaagcag ccaatggaaa aacttgtgaa tatttctatc aacatcaatt
     1261 cactttgaaa gatttaaaat taaatggcaa agaaatcatt gatggtgaat tcaagttaag
     1321 tgattgtgcg ctgacggata ttgctttcca tatggataaa atagccttaa atttaaaatt
     1381 aacaacacca tccgagattg cagaactgta cgcaagggat gaaatagcac aaattcgttc
     1441 aagcaaccgg ttctatttgt tttttaaatt attccggcca aaattaatca attggttaga
     1501 gaagcaaaat gacctggagc ctcaagtgac ttatcgtttc tcagggcaaa gttcttctgc
     1561 ttgatatcat taacttaaca aacccagtac aactagcaat ttacttgctc tgttctatac
     1621 aaggtaaagc gccctgattt atgaaaaaat ataaatctaa ccgttctttt atttttcaaa
     1681 aataaggcta aaataataaa taactatata aaaaggatat ttaaatggtt cgcctcagaa
     1741 tttatttatg ttctttgcta ctggttattc ccttttatag agcctatgct cttaatttga
     1801 ccatagggac cttatcttat aatcctccgt ttgaaactca agtcaacgat tcttccaaga
     1861 aaaatgagtt ttatggtttt gaaattgaac tcatggatga gatttgcgag cgcatcaatg
     1921 caaaatgcca atacaagcca ttgttatttg aacaaatttt agctgaaatt aattctggtg
     1981 caatcaattt aggcattggt agcattaata ttacgcctga aagaagcgaa cgatttttat
     2041 tcagccttcc ctacaaagcc agctatcacc agtttcttat accatctaac tcgaatataa
     2101 aaaaaattga cgatctcaaa ggaaagacta taggaattca tttaggttca cctactgccg
     2161 attatttaaa agcctacttt aacaataata ttcaatttaa atattacgaa acatttatgg
     2221 atttacttaa tgggctgaca gatccgcaag ttagcgcaat agtgactaat agcgatcaag
     2281 cgcaatactg ggcatcgagt acaccggact gcaagttatt gggcgacagt tttccttttg
     2341 gagtaggatt tggagttgtc gcgagattag atcaagcaga actgattgaa aaaatcaatc
     2401 aggcattaat tgagattgaa aatgatggga cttaccttaa aatatataac ct
//
LOCUS       DACTJE010000255         2452 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.257, whole genome shotgun sequence.
ACCESSION   DACTJE010000255 DACTJE010000000
VERSION     DACTJE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.257"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..623
                     /gene="sidD"
                     /locus_tag="I9760_002951"
     CDS             <1..623
                     /gene="sidD"
                     /locus_tag="I9760_002951"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T4SS effector deAMPylase SidD"
                     /protein_id="HAT5044688.1"
                     /translation="QVVKSKQKQLVEQAKETGQIQRFGDDFTVGRLVIPDQLLINQLR
                     IHALSIGVSDGLLSYIKNENENKGFLGIYGFFTGADKNIEKATLYKNLIAKYQNNHFI
                     SLIILSALVSDSKTPLMTQYLVGYLDFPSKALLANKITELLLKELENPDMREILGSRL
                     ATDVIEELETKIIRYIHNPAGSDIHSTLNLWTADKIKAATNSSLTI"
     gene            complement(652..1131)
                     /locus_tag="I9760_002952"
     CDS             complement(652..1131)
                     /locus_tag="I9760_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HAT5044689.1"
                     /translation="MNLIKILGIGSPFGDDQVGYLLADCLKTELSKDQYISKLVSIES
                     HDRPGLRLIEVMNPAKVVFLIDAVKSGCAIGTIHRLKNNEIFAFQNRLSTHEIGVVEA
                     LQIGQSLNALPEDIILYGIEIDSIAFNATLSKSVEKAMKVVVIQIKKELKDMLNHIV"
     gene            complement(1128..2420)
                     /locus_tag="I9760_002953"
     CDS             complement(1128..2420)
                     /locus_tag="I9760_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HAT5044690.1"
                     /translation="MGKDISINVPILARVEGEGALELCIRDSKIETLKLKIYEPPRLF
                     EKFLEGRSYNEVLDFVARICGICPVAYQMSATQAIEQCFNLSPTPWVRDMRRLFYCGE
                     WLESHSLHVYFLALPDFLGFQSAPEMARKYPEEVKCGLRIQAFGNDLIKLLGGRSVHP
                     VGACVGGFYKAPSHPQIKLILDKAKERISDCEALIHWLASLSLPDNSHDFTSVSLYHP
                     TEYPMNEGRIVSDRGLNITKEEFDAYFKEHHVDYSNALHCLLQNKPYLVGPLARINNC
                     FGHLPAPIHLLLQSLNIQFPSRNMCHSIIARAVEMYYCVLEAIRILEQYDLPEQSRPE
                     IKPQAGEGAGCTEAPRGMLWQHYVFDETGQVLQARIVPPTSQNQARIEEDLKHSLIQF
                     GLNQEEEAIRYFSEMIIRNYDPCISCSTHFLNLKIDRI"
BASE COUNT          810 a          501 c          412 g          729 t
ORIGIN      
        1 tacaagttgt aaaatccaaa caaaaacagc tggttgaaca agcaaaagaa acaggacaaa
       61 tccaacgttt tggtgatgat tttacagtag gaagattagt cattcctgat cagttactaa
      121 taaatcaatt aagaattcat gctttatcaa tcggagtaag tgatggatta ctttcataca
      181 tcaagaatga aaatgaaaac aaaggttttc ttggtatata tggtttcttc acaggagctg
      241 ataaaaatat agaaaaagcg actctttata aaaatctcat cgcaaaatac caaaataatc
      301 attttatctc tctaattatt ctttctgctc tagtctctga tagtaaaaca cccctaatga
      361 ctcagtactt agttggctat ctggatttcc cgtcgaaagc attacttgct aataaaatta
      421 cagaattgct tttaaaagaa ttagaaaatc cggatatgag agaaatactg ggttccagat
      481 tagcaactga tgttatagag gaattagaaa ctaaaattat tcgttatatt cacaacccag
      541 caggctcgga tattcattca acgctaaacc tttggactgc agataaaatt aaggctgcaa
      601 ctaactcgag tcttactatt taagtattat aagttcatta tgaaaagaaa ctcatacgat
      661 atgatttaac atatccttga gttccttttt tatttggata acaacgacct tcattgcttt
      721 ttcaactgat ttggacagcg tcgcattaaa agcaatgcta tcaatttcaa tcccgtataa
      781 aatgatatcc tctggcaaag cattaagact ttgacctatt tgtagtgctt ctacaacccc
      841 tatttcatgg gtcgataatc tattttgaaa tgcaaaaatc tcgttatttt ttaagcgatg
      901 gattgtacct atggcgcatc cggactttac agcatcaatt aaaaaaacga ctttggctgg
      961 attcatcact tcaatcaaac gaagaccagg ccgatcgtga gactctatgc tcaccagttt
     1021 actaatgtat tgatctttgg acaattctgt cttaagacaa tcggcaagca aatatcctac
     1081 ttgatcatca ccaaaaggtg agcctatgcc taatattttt ataagattca tattctatcg
     1141 atttttaaat tcagaaaatg tgtagagcat gaaatacacg gatcataatt acgaatgatc
     1201 atctcactaa aatacctgat cgcttcttcc tcctgattaa ggccaaattg tattaaagaa
     1261 tgctttaaat cttcctcaat acgcgcctga ttttggctgg tgggaggaac aatacgcgct
     1321 tgtaaaacct ggccggtttc atcaaaaaca taatgctgcc acagcatacc tctgggagcc
     1381 tcggtacaac ctgctccttc tcctgcctga ggtttgattt caggacgaga ttgctctggc
     1441 aagtcatatt gttccagaat gcggatagct tccaaaacac agtagtacat ctccactgct
     1501 cgagcaataa tactatggca catattccgc gaaggaaatt gaatattcaa gctttgtaaa
     1561 agaagatgaa tgggtgctgg caaatgccca aaacaattat taatacgggc cagaggacct
     1621 actaaataag gtttattttg caataaacag tgcaatgcat tggagtaatc cacatgatgc
     1681 tctttgaaat aagcatcaaa ttcttctttg gttatattta atccacgatc tgaaacaatc
     1741 cttccctcat tcataggata ttcagtaggg tgataaagcg aaacactggt aaaatcatga
     1801 gaattatcgg gtaaggataa gctcgcaagc caatgaatta acgcttcgca atccgagatc
     1861 cgttcttttg ccttgtctaa tattaatttg atttgcggat gggaaggcgc tttataaaaa
     1921 ccacctacgc aagctcctac tggatgaacg gaacgccccc ctaacaattt aatcaaatca
     1981 ttaccaaaag cctgaatccg caacccgcat ttcacctcct ctggatattt ccttgccatt
     2041 tcaggtgctg attgaaatcc taaaaaatca ggtaaagcca aaaaatagac gtgcaaacta
     2101 tgactttcca gccattcgcc acaataaaac aggcgacgca tatcgcgaac ccaaggagtg
     2161 ggacttaggt taaaacattg ttctatagcc tgggtagcac tcatttgata agcaacaggg
     2221 catataccac aaatacgggc tacaaaatcc aacacttcat tgtaacttcg accctccaga
     2281 aatttctcaa ataatcttgg gggttcataa atttttaatt ttaatgtttc aattttgcta
     2341 tcccgaatgc aaagttcaag agccccctct ccttccaccc gcgccaaaat tggaacatta
     2401 attgaaatgt ctttacccat tgctaatttt aattccctta aagtaatttc cg
//
LOCUS       DACTJE010000256         2422 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.258, whole genome shotgun sequence.
ACCESSION   DACTJE010000256 DACTJE010000000
VERSION     DACTJE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.258"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(103..726)
                     /gene="ravG"
                     /locus_tag="I9760_002954"
     CDS             complement(103..726)
                     /gene="ravG"
                     /locus_tag="I9760_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavG"
                     /protein_id="HAT5044691.1"
                     /translation="MKNIYPGRVICDMKNAAIIKNRIFLNLDKPVKRFLASDKADTPM
                     TAEFYAEKDYEQLFLDFLSQATGSYDEQISLLIAELDSGADRVAQKLMLALYSPWQKN
                     LLPKAIKTIANKSEEYPLMSDLLIKFCQQHVGSVDAVDDFGETALAKILKKDQQRKSP
                     LLFLVKHGAKHCQLTSALQDSLIVNNSDIYNVAEDNTMDWISNCPQP"
     gene            complement(764..>2422)
                     /locus_tag="I9760_002955"
     CDS             complement(764..>2422)
                     /locus_tag="I9760_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044692.1"
                     /translation="EHADELNALCLVPIKDSQSNENNILPRRFESELACRLIESPTKS
                     MMQAIDLVSQAIINLIEEYSKLDSKSSERTALNFFLQELEGSDIEFGAFKKRPSLEEV
                     IAILKENNPKNFIQIQFISFMFARSVIGPFPLRNLPPFKPTGLFAQVLDKYLQAHSDL
                     TEEEEGFSYQLMAAKNEAEEFRTFITKKLFYQDELYTQGENRGRQGPRKTYLKTNQLG
                     LMKLDHSAHSVGLTVIPEQIWCADAQAQEANQNSIYYKTALTNDCPYVTGPSGMTSIY
                     MNMMLLLLNPQNIELIQAYSLGIMAYIVGAGFHSVNEILIPLVKCINLLPNYPSYEGL
                     EYLTHPPLYHEYFKQIAQFDSDFIQLRDQVWAYYLNYFKSCYMPLCMRDFCEAEQLAN
                     CPDPAPEVKILCGIVLKSIKDCINFHNLNRHLDSRFLGTTTADIDALNQLKEACASQS
                     SISGIMRLLQKYFSGENIARSTAMHKFETRSYIRFFLDALKDNPQIISHINEKMKLDP
                     PLVISTKAEIFSDDFRIECFNLHRILKREYQVKEDCKNTSQLKL"
BASE COUNT          751 a          457 c          438 g          776 t
ORIGIN      
        1 actaaatgac cgaattaaaa acatttttaa catagagatt gggtttttcg taagtcctta
       61 agcaatcatc tttgagccaa cagaatattg ccctctgaag aattagggtt gagggcagtt
      121 ggaaatccaa tccatagtat tatcttccgc gacattgtaa atatccgaat tattaacaat
      181 aagagaatct tgtaacgcag aagtgagttg gcaatgtttt gcgccgtgct tgactaaaaa
      241 taaaagaggg gacttccttt gctgatcttt ttttaagatt ttggctaggg ctgtctcgcc
      301 aaaatcatca acagcatcaa cagagccgac atgttgctgg caaaatttaa ttagcaaatc
      361 actcatcaag ggatattcct cagatttgtt cgcaatggtc ttaattgcct tcggcaatag
      421 attcttttgc catggcgagt aaagggctaa cattaatttt tgcgcaacac gatctgctcc
      481 tgaatccaat tcagcaatta gcaagctgat ttgctcatca taactaccag tagcttggga
      541 taaaaaatct aaaaagagct gttcgtaatc tttttcggca taaaattccg ctgtcatggg
      601 agtatctgct ttatcactcg ccaaaaagcg cttaacaggt ttatctaggt ttaagaaaat
      661 tcgatttttg ataattgctg catttttcat atcacaaatt accctacctg gatatatatt
      721 tttcattata atgaagttta agggatattt aaaccacttt cctttacaac tttaactggc
      781 tagtgttttt acaatcttct ttcacctggt attctctttt tagaattcga tgcaaattaa
      841 agcactctat tcgaaaatca tctgaaaaaa tttcagcctt tgtactaatt actaatggag
      901 gatccaattt cattttctca ttgatatgag atatgatttg cgggttatct tttaaagcat
      961 caaggaaaaa acggatataa gaacgagttt caaatttatg catcgctgtt gagcgagcaa
     1021 tattttcccc tgaaaaatat ttttgcaata atcgcataat accgctaatt gaagattgac
     1081 tcgcgcaagc ctccttcaat tgattaagag catcaatgtc cgcagtagtc gtacctaaaa
     1141 acctgctgtc cagatgccta ttaagattat gaaaattaat gcagtcctta attgatttaa
     1201 gcacaatacc gcataatatt tttacttctg gagcagggtc agggcaattt gcaagctgtt
     1261 cagcctcgca gaaatctctc atacataagg gcatatagca cgatttaaaa taatttagat
     1321 agtatgccca tacttgatct cttaactgaa taaaatcaga atcaaattgt gcaatttgct
     1381 taaagtattc atgataaaga ggaggatgag ttaaatattc aagcccctcg tagcttggat
     1441 aattaggcag aagattaatg catttcacca ggggaataag aatttcgttg acactgtgaa
     1501 aacccgcacc gacgatatat gccataattc caaggctgta agcttgtatt aattcgatat
     1561 tttgaggatt taaaagcagc agcatcatgt tcatatagat agaagtcatg ccagaagggc
     1621 cagtaacata aggacaatca tttgtaagtg cagtcttgta atatatcgaa ttctgattgg
     1681 cttcttgtgc ttgagcatcc gcacaccaaa tttgttcagg aataacggtt aaccccacgc
     1741 tatgcgctga atgatccagc ttcatcaatc ctaactggtt ggtttttaag taggttttac
     1801 gaggcccttg acgccctctg ttttctcctt gggtataaag ttcatcctgg taaaataatt
     1861 ttttagtgat gaaggttcta aattcttccg cctcattttt agctgccata agctgatagg
     1921 aaaagccttc ctcttcttct gtcaaatcgg agtgagcctg taaatattta tccagaactt
     1981 gggcaaaaag gccagttggc ttaaatggtg gcaaatttcg taatggaaat ggtccgatta
     2041 cactgcgagc aaacataaaa gatataaatt gaatttgaat aaaattcttc ggattatttt
     2101 cctttaaaat agcaataact tcttccagag atggcctttt tttaaaagcc ccaaactcta
     2161 tatcagaacc ctctaactct tgcagaaaaa aattcagcgc agttcgttcg gaagatttcg
     2221 agtctaattt actatactct tcgattaaat tgattatagc ttgagatacc agatcaattg
     2281 cttgcatcat gcttttcgtt ggtgattcta tcaagcggca agccagctct gattcaaatc
     2341 tccggggcag aatattgttt tcattagatt gactgtcttt gataggcacc aagcataacg
     2401 catttaactc atcggcatgc tc
//
LOCUS       DACTJE010000257         2418 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.259, whole genome shotgun sequence.
ACCESSION   DACTJE010000257 DACTJE010000000
VERSION     DACTJE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.259"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..>2418
                     /gene="sdeB"
                     /locus_tag="I9760_002956"
     CDS             <1..>2418
                     /gene="sdeB"
                     /locus_tag="I9760_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdeB"
                     /protein_id="HAT5044693.1"
                     /translation="ETLGQITTDAMKDELTDNGYLKITGWLKAVADKFPEGDPQPDAK
                     ALRQTTEKDLHIEIPSPVSPVKDTAPKEVSTKPTAPQVAPKYSLDSKLLENDDDVLDT
                     IKYVHKEYLGKPYPGPLKNPKAPEEGRLPPNEGPDRGPHGLAHTVRTMACAEVMIEEA
                     RKAQLRGETLGKAKNGQTLADVTPEELKKILIAQAFFVVGRDDERSGYDDVHKRNFYA
                     EYHEKSEQAFRKYVEDNKLIGKIFKDQKEVDFYAAIILDKNHEWDATPAHILINQGHM
                     VDLMRTKAPAEVALERTYNTLKGTVGSKGAEVVLKAHRDFFFATGAVVPLVNPEAIDD
                     PSRGGPYENPYSGEKFVIVDDKVPASKKDLPKAVNRDYKLKDNERFLTIKEYYAFPDV
                     QQTYPGYKTRLEASSYYFPTPFAGECEQNPAKCLGAIQKARSKLQTDAIKNGFQSSSE
                     KERRQPNMDEIAAARIIQQIMANPDCIHDDHVLINGQKLEEKFFRDLLAKCDMAVVGS
                     LLNDTDIKNIDTLMRHEKNTEFHSTDPKAVPVKIGDAWENRIRTKGGDVTQMKHDLIF
                     LMQNDAWYFSRVNAIAQNRDKGSNFKEVLFTTLMTPLTNKSLIDTSHVPAPKKLYRGL
                     NLPQEFTNKLINQSNAIIANTENTLFTDLSAEAFKQIKLNDFSQMSGKTCASTTKNMK
                     LLTDIWGSNVIFEMLDPDGLLHPKQVGTHMTGSEDEFSVYLPEDVALVPTKVTLEGKT
                     DTGEDRYIFTLVAVKSPDFIPRHESGYAVEPFMKMQKEKVTQALDAIEKDKDSYNIDE
                     QLKSLRT"
BASE COUNT          844 a          508 c          483 g          583 t
ORIGIN      
        1 agaaactttg ggtcaaatca caacggatgc aatgaaggat gaattaaccg ataatggtta
       61 tctgaaaatt accggctggc taaaagctgt tgctgataaa tttccagaag gtgaccccca
      121 acccgatgcg aaagcattaa gacaaaccac tgaaaaagat ttgcacatag agataccctc
      181 tcctgtttct ccagtcaaag acacagctcc taaggaagtt tctacaaaac ctactgcacc
      241 acaagtagct cccaagtatt ctttagacag taagttatta gagaatgacg atgatgttct
      301 agacaccata aagtatgttc ataaagagta tttaggaaaa ccatatcctg gcccattaaa
      361 aaatccaaaa gcaccggaag aaggacgact tcctccaaat gaagggcccg atcgaggacc
      421 tcatggtctt gctcatacgg taagaacaat ggcttgtgcc gaggtgatga tagaagaagc
      481 acgtaaagct caactgagag gagaaacatt aggtaaagca aaaaatggtc agactttagc
      541 tgatgtaaca ccagaagaac tgaaaaaaat tctcatcgca caagcctttt ttgtggtagg
      601 aagggatgat gagcgttccg gctatgatga cgtccacaag agaaattttt acgcggaata
      661 tcatgagaaa agtgagcaag cgttcaggaa atacgttgaa gataacaagc tcatcggcaa
      721 aatatttaaa gatcaaaaag aagtcgattt ctatgctgcg attattctgg ataaaaatca
      781 tgaatgggat gccaccccgg cacatatcct gataaatcaa ggccatatgg tcgatttaat
      841 gaggactaaa gcacctgccg aagtcgcatt ggaacgtacc tacaatacac tgaaaggtac
      901 cgtcggttca aaaggtgctg aagtggtcct caaggcccat cgagattttt tctttgccac
      961 aggcgctgtt gttcccttag ttaaccctga agcaattgat gaccccagta gaggtggacc
     1021 ttatgaaaat ccttatagcg gggagaaatt tgttattgtt gatgataagg taccagcttc
     1081 caagaaagac ttaccaaaag ctgtcaatcg tgactataaa ctgaaagata acgaacgatt
     1141 cctaaccata aaggaatact acgcttttcc tgatgtgcag caaacctacc ctggttacaa
     1201 aacacgtttg gaagcaagct cttactattt cccaacacca tttgcaggag aatgtgaaca
     1261 aaaccctgca aaatgtctgg gagctatcca aaaagcccgt tcaaaactac aaacggatgc
     1321 cattaaaaat ggctttcaat caagctctga gaaagagaga agacaaccta atatggatga
     1381 aattgcggcc gcccgcatta ttcaacaaat catggccaat cctgattgca tccatgacga
     1441 tcatgtgctc atcaatggtc aaaaactgga agaaaaattc ttccgtgact tgttagcgaa
     1501 atgtgatatg gctgttgtag gctcactgct caatgacacc gacatcaaaa atatcgatac
     1561 tttaatgcga cacgagaaaa acactgagtt tcattctact gaccccaaag cagttccagt
     1621 gaaaattgga gacgcctggg aaaatagaat aagaaccaaa ggtggcgacg tcactcaaat
     1681 gaagcatgat ttaattttcc tcatgcaaaa tgacgcctgg tactttagcc gagtcaatgc
     1741 catcgcacaa aaccgggata aaggctccaa tttcaaagaa gtgctgttta ccaccttaat
     1801 gacgcctttg actaataaat cattaataga tacatcccac gtcccagctc cgaaaaaact
     1861 gtatcgtgga ttaaatctgc cacaagaatt taccaataaa ttaatcaatc aatccaatgc
     1921 cattattgcc aatacagaaa atacgctttt tactgatctc tctgctgaag ccttcaaaca
     1981 aatcaagtta aatgatttta gccaaatgtc tggcaaaacc tgtgccagca caaccaaaaa
     2041 tatgaaactt ttaaccgata tatggggctc caatgtcatt tttgaaatgc ttgacccgga
     2101 tggtttactg catccaaaac aagtaggaac ccatatgact ggatcagaag atgaattttc
     2161 cgtttatttg ccggaagatg tcgctttagt tccaaccaag gtaactcttg aaggaaaaac
     2221 agacacagga gaagatcggt atatctttac actggttgct gttaaaagcc ctgactttat
     2281 accacgacat gaaagcggct atgcggttga acccttcatg aaaatgcaaa aagagaaagt
     2341 cacccaggca ttggatgcca ttgaaaagga taaagacagc tataacatcg acgaacaact
     2401 aaaaagctta agaacaga
//
LOCUS       DACTJE010000258         2411 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.260, whole genome shotgun sequence.
ACCESSION   DACTJE010000258 DACTJE010000000
VERSION     DACTJE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.260"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(27..1529)
                     /locus_tag="I9760_002957"
     CDS             complement(27..1529)
                     /locus_tag="I9760_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAT5044694.1"
                     /translation="MISQAVKNYLPELSLRQKQTNNIIFITFWSQFSVYALNTILVLF
                     LTRPLIAQGLGYSQAKAYAFIGVTQATGYLMPILGGYMADNIIGVRRSILLGSIMLAC
                     AYLLVMLSGYTISSLGDQLFIIAFAFIPATNSLLMGTASSMVSHIYSDDAIKAKSAMT
                     YYYMAINVGALLATMIAPVLLESRYGPLSVLTLTFIGKSIAALNFAKRYSIYDSVIWG
                     MDKSKFSNQGLLRLVAYIAAIYSLTLYAYTHVYIASTLIGAGCAIGILWFLIKTIMLK
                     GETRSKQMIAVLLIIEAIVFFIIYNQMNSTLVLFAENNSDLSFLWFKISPAQYQMLNP
                     LLILLIGSQLPRFYRFFSRFTIPYQFAAGTILSGIALLVMAFAAQNAINGLVNGNYIA
                     LTYILISIAELWVSAIGLSMIGLYCDARAIAFAMGVWYLASSMSNAISGRIAGWVAIP
                     ENINSALESLPYYKNYYLIMGISALGLGILMYFVAYYLHKIMKRRGIELA"
     gene            complement(1574..2392)
                     /locus_tag="I9760_002958"
     CDS             complement(1574..2392)
                     /locus_tag="I9760_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5044695.1"
                     /translation="MSLKAMYNQIAENYATANRFGSISESHHVAIEQMRKFHLGLKPH
                     YKILDLGVGNGSFLQKLHHLMPMANFTGIDVSSEMLKRASEALPLTTIEGSAAEANKF
                     LPAHSQDLVLAHFINAYIPINVLFDEAKYLTRANGHFSLITTTYDSFPVAQQQLANFI
                     AQDTILSRVVGHYYKSIVKNTTVAANLDELLLAFKQHQFNILDHQRIEIPITLNNIDE
                     LALFGIEGTWFLNTLSIRMLPKYFLIQRLKRLFSKIFTFPYHDTHIIDVVLARK"
BASE COUNT          811 a          449 c          484 g          667 t
ORIGIN      
        1 ttcgaatatt acaatgttca gttacgttat gctaactcaa tacctcgtcg tttcataatc
       61 ttgtgtaaat aataagctac aaaatacatc agaataccaa gccccaaagc actaatgccc
      121 ataatcaaat aataattttt ataatagggt aaactttcaa gagcagaatt aatattctct
      181 ggtatggcga cccaacctgc aattcgtcct gaaatggcat tggacatgga actggccagg
      241 taccatacgc ccatcgcaaa ggcaatcgca cgtgcatcac aatacagtcc aatcatgcta
      301 agaccaatgg cactaaccca taattcggca attgaaatta aaatgtaggt caaagcaatg
      361 tagttaccat tcactaatcc attgattgca ttttgggctg caaaagccat aaccagcagg
      421 gcgataccag agagaattgt gcctgcggca aattgatagg ggatcgtaaa gcgcgaaaaa
      481 aatctgtaga aacgaggaag ttgacttcca atcagtaaaa tgagcaaagg atttagcatt
      541 tggtattgag cgggtgaaat tttaaaccaa agaaaactta gatcagaatt gttttctgca
      601 aataatacca aggtactgtt catttgattg tatatgataa aaaatactat agcttctatt
      661 atcagtaata cagcgatcat ttgtttactg cgagtttcgc ctttaagcat aatggtttta
      721 attaaaaacc agagaattcc tatagcacat cctgcgccta tcagtgtgct ggcgatataa
      781 acatgagtat aagcatataa agtaagagaa tagatagccg ctatataagc aacaaggcgc
      841 aataggcctt ggttagaaaa tttggattta tccatacccc agatgacact atcatatatg
      901 gagtaacgtt tcgcaaaatt caaggcagca atcgatttgc caataaaggt taaggtgagt
      961 actgaaagtg gaccataacg actttctagt agaactggcg cgatcatggt tgccagcaga
     1021 gctccaacat taatagccat ataataataa gtcatagcgg atttggcttt aatggcatca
     1081 tcggaataaa tatgagaaac catactggat gcggttccca ttaatagcga gttggttgca
     1141 gggataaagg caaaagctat gataaataat tgatccccaa gagaactgat ggtgtaccca
     1201 cttaacatga ccagtaaata agcacaagcc agcatgatgc tacccaaaag aatggatcgt
     1261 ctgacaccaa taatattatc agccatatac ccacctaaaa taggcatcag gtagcctgtg
     1321 gcttgagtta caccaatgaa agcgtacgct ttggcttggc tgtagcctaa tccctgagca
     1381 atcaagggtc gagttaaaaa taaaaccaga attgtattca gggcatagac agaaaactgg
     1441 ctccaaaaag taataaaaat aatattattg gtttgttttt ggcgaagact tagttctgga
     1501 agataatttt taactgcttg agaaatcaat ttcacacctc attccgtttt ttctaaccca
     1561 ccgcccaagg ttatcatttc ctggcaagaa ctacatcaat aatatgagtg tcatggtagg
     1621 gaaaagtaaa gattttgctg aaaagtcgtt taagacgctg aattaaaaaa tattttggca
     1681 gcatgcgtat cgatagagtg ttaagaaacc aggtgccttc tattccaaac agtgccaatt
     1741 catcaatgtt attaagggta atgggtattt caatacgttg gtgatccaaa atattgaatt
     1801 gatgctgttt aaatgccagc aagagttcgt cgagatttgc agcaacagtc gtatttttga
     1861 ctatagattt atagtagtga ccaacaactc gacttaaaat agtatcctga gcaatgaagt
     1921 tggccagttg ttgttgcgct acaggaaaag aatcataagt cgttgtgatt aaagagaagt
     1981 gaccgttggc gcgcgtcaag tatttggctt catcaaagag aacattaata gggatataag
     2041 cattaataaa atgagccaga accaagtcct ggctatgtgc gggcaaaaac ttgttggctt
     2101 ctgcagcact gccttcaatg gttgttaaag gaagggcttc gctcgctcgt tttaacattt
     2161 ctgatgaaac atcaattcct gtgaaattgg ccattggcat taggtgatgc aatttttgca
     2221 aaaaagaacc attgccaacg cccagatcga ggattttata atggggtttc aaccccaaat
     2281 gaaatttcct catctgctca atggcaacgt gatggctttc actaattgaa ccaaaacgat
     2341 tagctgtcgc gtaattttct gcgatttggt tatacatggc cttcaaggac atgcaaaatt
     2401 cctactttaa c
//
LOCUS       DACTJE010000259         2408 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.261, whole genome shotgun sequence.
ACCESSION   DACTJE010000259 DACTJE010000000
VERSION     DACTJE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.261"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1728
                     /gene="sidJ"
                     /locus_tag="I9760_002959"
     CDS             <1..1728
                     /gene="sidJ"
                     /locus_tag="I9760_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS meta-effector polyglutamylase SidJ"
                     /protein_id="HAT5044696.1"
                     /translation="NFYHSTLTDNLLLRTEPKEVTLSSEHHYKKEKGPIDSSFRYQMS
                     SDRLLRIQGRTLLFSTPQNDVVAVKVQKKGEPKSTLEEEFEMADYLLKHQRRLDVHSK
                     LPQPLGQYSVKKSEILEISRGSLDFERFKTLIDDSKDLEVYVYKAPQSYFTYLHDKNQ
                     DLEDLTASVKTNVHDLFVLLREGIVFPQLADIFHTHFGEDEREDKGRYQALVQLLNVL
                     QFQLGRIDKWQKAVEYVNLRSSGLADLGDSLPITSLFTSSDFTKHYFSELLTGGYHPT
                     FFDKSSGTANSLFTGKRRLFGNYLYLNTIAEYLLVIQLTLGSYGDKVTRDMMDKPKKE
                     AVWRELANVMFTSCAEAIHIMTGIPQSRALTLLKQRANIEKHFRQTQFWMTPDYSKLD
                     EDTLQMEQYSIYSGEPEYEFTDKLVSGVGLSVDGVHQDLGGYNRESPLRELEKLLYAT
                     VTLIEGTMQLDKEFFKQLEQVEKILSGEIKTDANSCFEAVAQLLDLARPGCHFQKRLV
                     LSYYEEAKLKYPSAPTDAYDSRFQVVARTNAAITIQRFWREARKNLSEKSDIDSEKPE
                     SERTTDKRL"
     gene            2009..>2408
                     /locus_tag="I9760_002960"
     CDS             2009..>2408
                     /locus_tag="I9760_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HAT5044697.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGHPEIKSGTIYNGVPTIDQEAL
                     DKQGFMPVLTGVGPKRDSGHWIMLIKGSGNQYHLFDPMGKISGEGYQDILATQLPKGS
                     TLSVIPNEPGLNKGLCGYWVASVGLKACSAL"
BASE COUNT          774 a          493 c          477 g          664 t
ORIGIN      
        1 aacttttatc attccactct gaccgataat ctcttgttaa gaacagaacc taaggaagta
       61 actctttcat cagagcatca ttataaaaag gaaaaaggac caattgactc gtcttttcgt
      121 tatcaaatgt caagtgatcg gttattgcgt atccagggca gaactttact gtttagtacc
      181 ccccaaaatg atgtggttgc agtcaaagta caaaaaaaag gagagcctaa gtccaccttg
      241 gaagaagaat tcgaaatggc agattatttg ctgaaacatc aacgtcgttt ggatgtacac
      301 agcaaattgc cacaaccgct tggacaatac tctgttaaaa aatcagagat tttggaaata
      361 agcaggggat cattagattt tgagcgtttt aaaaccttaa tagacgactc taaagacctt
      421 gaagtgtatg tttataaagc cccccagtct tatttcactt acctacacga taaaaatcag
      481 gatttggaag atctgacagc ttctgttaaa acaaatgtgc acgatctctt tgttctatta
      541 cgcgaaggaa tcgtatttcc ccaattagcc gatatcttcc atactcattt tggtgaagac
      601 gagcgcgaag acaaaggaag ataccaggca ttagttcaac ttttgaatgt tttacagttc
      661 caattagggc gcattgataa atggcaaaaa gctgtggaat acgtcaattt gcgcagcagt
      721 ggtctggctg atttgggaga cagtctgccc ataactagcc tgttcacttc atctgatttc
      781 acaaaacatt atttctctga actgcttaca ggaggatatc acccaacctt ctttgacaag
      841 tcatctggta cagccaactc tttattcacc ggcaagcgcc gattatttgg taattacttg
      901 tatctcaata ccatagcaga atatttgctg gttattcagt taacactcgg cagctatggg
      961 gataaagtga ctcgagacat gatggataaa cctaaaaaag aggcggtatg gcgagaattg
     1021 gcaaacgtca tgtttacaag ttgtgcagaa gccattcata tcatgactgg aatacctcaa
     1081 tcgcgagcac tgacactact aaaacaacga gctaatattg aaaaacattt cagacagaca
     1141 caattctgga tgacgcctga ttattccaaa ctggatgaag atacactgca aatggagcaa
     1201 tacagcattt attccggtga gccagaatat gaattcactg ataaattagt ctctggtgtt
     1261 ggattgtccg tcgatggtgt tcatcaggac ttgggtggat ataacaggga aagcccatta
     1321 agagaacttg aaaaactgct ctatgccaca gtaacactga ttgaaggaac catgcaactt
     1381 gataaagagt tttttaaaca attggaacaa gttgaaaaaa tactttctgg tgagataaaa
     1441 actgatgcca attcgtgctt tgaagcagtt gcccaacttc tggaccttgc aaggcctggg
     1501 tgccattttc agaaaagatt ggttttgtcc tactacgaag aagccaagct taaatatccc
     1561 tccgcaccaa ctgacgccta tgattcacgt tttcaagtag tcgccagaac caatgccgca
     1621 ataaccattc aacgattctg gcgagaagca cgcaaaaact tgtcagaaaa atcggatatc
     1681 gattctgaaa agccggaatc cgaacgtact actgataaac gtttgtgata aaacgaatac
     1741 cctgcccctg actctttgaa accacactat tcccatggag aagtctataa gacagaatag
     1801 acttcttcat tttctggctt gagttttaag agcaataaga gaaaaccccc cgcttctgtt
     1861 ccagctatca actccatatg ttgcctatct tttaaacact taatactttc ttaatactgt
     1921 agccctataa tgaaaataaa cataagaata ttcacatcta ttagaatcag tatttgtaca
     1981 tacaaatatt cgcttgggag aaagtaaaat gcctaaatat gtagaagggg tagagttaac
     2041 tcaagaaggc atgcatgcta tttttgcacg gatggggcat cccgagatta aaagcggtac
     2101 tatttataac ggagtaccta ctattgatca agaagctctc gacaagcaag gttttatgcc
     2161 tgtattaaca ggggtaggac ctaagagaga ctccggtcac tggatcatgt taatcaaagg
     2221 atcaggcaac caataccatc tctttgatcc aatgggtaaa atttcaggtg aaggctatca
     2281 agacatttta gccactcaat tgcccaaagg ctccaccctt tctgttatcc ccaatgagcc
     2341 cggcctaaac aaggggctat gtggctattg ggttgcttct gttggattaa aggcatgttc
     2401 tgcactca
//
LOCUS       DACTJE010000260         2408 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.262, whole genome shotgun sequence.
ACCESSION   DACTJE010000260 DACTJE010000000
VERSION     DACTJE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.262"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            34..903
                     /locus_tag="I9760_002961"
     CDS             34..903
                     /locus_tag="I9760_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1685 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044698.1"
                     /translation="MQCAIERKNSLRDLQSQPNLGDWMFISSLGSQDFDERKFFSQIS
                     LQSLSFIIRIAKFSAYFAALCEKSEYDELWKKLYSTFGLVLTRDKPVPKSFFTHDGGE
                     INHFNLLRGAYYFHLSQKALAAKEKSFSDSELYWLNQAMKFGSIHASQRYIQFLYQKL
                     DKVASADEAEQILTEAIKLCKANLNQYGSYAYMMLAEAFFRYAMWAQQSGNINRAKSA
                     ITSSINSCTKANQHLRASASSIHNASLGEGLKASNTLAMESPEEAIAFLNAWAVAHLY
                     KHEIELVSRPGFY"
     gene            complement(914..2311)
                     /locus_tag="I9760_002962"
     CDS             complement(914..2311)
                     /locus_tag="I9760_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1684 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044699.1"
                     /translation="MKSVDEIDSLLQSAKTSEKDSSEQIVPPNDEIEDGFYLIDDSNH
                     DEDLDFSDFPWLDPDHPLFFPATQANNSKGSTRWDAVNKFSVENWYPHLRKHTFKSHF
                     ITLNYNDIHYLMGNIPPDYDSSKLESIFNKILSEFNNKEVFMRLSTRSPKDSKFLFEE
                     ASTIMSKDFVYWNENDNKHQQLVSFVASMLKAMKIKSGRKIIETIAQSPRVYNDLLAL
                     VSSSNQSDCTTNVILREWHDIRPDHEFRVFVSRRHRKESIVTAISQYFHFLYFDKSPA
                     DCFNFLDEEDKKAVIKKFENYVLKSVDPDVAKFLNFSSEQDDDESSDCIREYIVDLAL
                     IPVSQYHGEITDENIIEIGANTYVMVVIELNPFAPAATGSGLFNWKNDLMMLWGKASC
                     DYPVFKYRTTPREDLQSVSLLPSNYESVIKSALIKRLETSYSNSASQKERFFSSTKPV
                     TESSGSALLDMVTKP"
BASE COUNT          733 a          447 c          426 g          802 t
ORIGIN      
        1 gttatttggc aaagatgttg agggcttaaa aaattgcaat gtgctatcga gagaaaaaat
       61 agtttacgtg atttgcaatc acagccaaat cttggtgatt ggatgtttat ctcctcttta
      121 gggagccaag attttgatga gagaaagttt ttctctcaaa tttcattgca atcactgtct
      181 tttattatca gaatagctaa attttctgct tattttgctg cgctttgtga gaaaagtgaa
      241 tatgatgagc tttggaaaaa gctctactcg acttttggat tagttctcac cagagataaa
      301 ccagtaccta aatctttttt tactcatgac ggtggagaaa tcaatcattt taatctacta
      361 cgtggcgctt attattttca tctttcccaa aaagcattag cagcaaaaga aaagtccttt
      421 tctgactcag agctctactg gttaaatcag gcaatgaaat ttgggtcgat ccatgcaagc
      481 cagcgttata tccaattttt atatcaaaaa ttagataagg ttgcttctgc agatgaagca
      541 gaacaaattt taacggaggc aattaagctt tgtaaggcaa acttaaatca atatggcagt
      601 tatgcttata tgatgctagc tgaagctttt tttcgttatg cgatgtgggc acaacaatcc
      661 ggtaatataa atcgtgctaa atctgcaatt acctcgtcaa ttaattcctg cacaaaagct
      721 aatcaacacc tgcgtgccag tgcctcctcc attcataatg caagcttggg ggaggggttg
      781 aaagccagca atacacttgc tatggaaagc ccagaggaag ccatcgcttt tttaaatgct
      841 tgggcagtcg cgcaccttta caaacatgaa atagaattgg tctcccgccc aggtttttac
      901 taaatcttgc ctatcacggt tttgtaacca tatcaagcag ggctgatcca gaggattcag
      961 taacaggttt agtggatgaa aaaaatcttt ctttttgact tgctgagttt gagtaggagg
     1021 tttccaatcg tttgatcaag gcacttttga taacagactc ataatttgac ggcagcaatg
     1081 atactgactg taaatcttct cttggtgttg tacgatactt aaagacggga tagtcacagg
     1141 atgctttccc ccaaagcatc attaagtcat ttttccaatt gaataaacca cttcctgttg
     1201 ctgctggtgc aaatggattc agctctatga ctaccattac ataagtattt gcccctattt
     1261 caattatgtt ctcatctgtt atttcgccgt gatattgact gactggaatc aaggctaaat
     1321 caacaatgta ttcacgaatg caatctgatg attcgtcatc atcttgttcg cttgaaaaat
     1381 ttaaaaattt tgcaacatct ggatctacag atttgagaac ataattttca aattttttaa
     1441 taacagcttt cttatcctct tcatctagaa aattgaaaca atctgcaggg cttttatcaa
     1501 aatataaaaa atgaaaatat tgtgatatcg ctgtaacaat actttcttta cgatggcgtc
     1561 tggatacaaa aactctaaac tcatggtctg gacgaatgtc atgccattcg cgcaaaataa
     1621 cgtttgtcgt acaatctgat tgattgcttg aactaacgag cgcaagcaaa tcattataaa
     1681 ctctagggct ttgtgctatt gtttcaatta tttttctacc acttttaatt ttcattgcct
     1741 tgagcattga agcaacaaat gacaccaatt gctgatgttt attgtcattt tcattccaat
     1801 acacaaaatc cttactcatt atggtagaag cttcttcaaa gagaaatttg gaatccttgg
     1861 ggctcctggt ggacaatctc atgaagactt ctttattgtt aaattctgaa aggattttat
     1921 tgaaaatact ttccagttta ctgctatcgt agtctggagg aatgtttccc attaaatagt
     1981 gaatatcatt atagtttagt gtaataaagt gtgacttgaa ggtatgtttt cttaaatgag
     2041 gataccaatt ttctacagaa aatttattta ccgcatccca tcgtgtcgat cctttcgaat
     2101 tatttgcctg tgttgccgga aaaaataatg gatggtcagg atccagccat ggaaaatcac
     2161 taaagtcaag gtcttcatca tggttactat catctatgag ataaaatcca tcttcaatct
     2221 catcatttgg tggaacaatt tgctctgaac tgtctttttc acttgttttg gctgactgca
     2281 aaagagagtc tatttcatca acagatttca tgtttaccgc actccaccca acgctttgta
     2341 aatttaatat tcacaccata atccaataac atgacttatt aggatacaat atggccaatt
     2401 aattctaa
//
LOCUS       DACTJE010000261         2379 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.263, whole genome shotgun sequence.
ACCESSION   DACTJE010000261 DACTJE010000000
VERSION     DACTJE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.263"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..660
                     /locus_tag="I9760_002963"
     CDS             <1..660
                     /locus_tag="I9760_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1670 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044700.1"
                     /translation="EEHFSFEEYPPLTVSQLVKGIYFYGQAAECREEEGKPFGDNELE
                     FLKKSAYQHCFYAYNSLSTWAYEKYKMGLNDYSLLTLHYAQKACQYHWTPGYLLFYKT
                     CLNLAILSNAPSLSYQEALEALLIARKLSEHQYSISAINNAYFGKGLIHGNKTQIESW
                     DKAISETIAKGKIPSTLLNKIYDKASEKAKGILDEFTVEVKDMPEEEKLENEVAPTLS
                     I"
     gene            764..>2379
                     /locus_tag="I9760_002964"
     CDS             764..>2379
                     /locus_tag="I9760_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAT5044701.1"
                     /translation="MGYHFLTEGNGIIACYDMYPTQFHSIKDMINYLPVLKQMGFNAL
                     WINPMQMPGDISGFFKTDKNNGVKTGNEVTRSLYAMSHPLLFNPQFSLGSPEDPMGTT
                     QRLNSEALQLFTQNARNLGIVPMFDLVLNHVAIDSPLCQDKPHWFKGVHEDFKDVRGF
                     NYDDESIRKEIITEFWQPYIKRYMIEYGFDGVRVDAVGYVHPAVRSEIYAYIHSLAAE
                     YGKPKPVILDEALFSKRPLADEVNYLKLPGIGPTHITTEAYNVEFDLASADLPYEITL
                     EEQLKASVVFQQKDGTWRENTKGGCINFCGNHDYRSLAMTILFQMAKKRLESDAFYND
                     LMSSYQDLYFKFNAEQEKPHELKEPLKTTLLYSYVDQIKKELEDNKDETTQEFKTLVL
                     EKLALSALTASGGWFLLSGDETCDITAKTVFQRKGAVDKSYYPQREHRIFSEKPVIAH
                     KILEKMAEENFFIENSENKWMQELYRSLSSFPEMQKRLLVSHIDNIKHQINAGIDHVQ
                     EKFSRLLASENLNIGFTSQDFLENPRTHENG"
BASE COUNT          804 a          414 c          450 g          711 t
ORIGIN      
        1 gaagaacatt tttcatttga agaatacccg ccactcacag tgtcccaatt agtaaaaggg
       61 atctattttt atggacaagc agctgaatgc cgggaagaag aaggtaaacc atttggtgat
      121 aatgaattgg agtttcttaa aaagagcgct tatcaacact gtttttatgc ttataactct
      181 ttatcaacct gggcttatga aaaatacaaa atgggtttga atgattattc tttgttaacc
      241 ttgcactatg ctcaaaaggc ctgtcaatat cattggactc caggatattt attattttat
      301 aagacttgtt taaatttagc cattttatcg aacgcccctt ctttatctta ccaggaggca
      361 ttagaagcgc tcttgattgc ccgaaagtta tctgagcacc aatattctat aagtgctatc
      421 aataatgcct actttggcaa gggcctgatt catggcaata aaacgcagat agagtcttgg
      481 gataaagcaa tatcagaaac tattgccaaa ggcaaaatcc cctctactct gcttaataaa
      541 atttacgata aagcatctga aaaagccaaa gggatattgg atgagttcac agtcgaagta
      601 aaagatatgc cagaagaaga aaaattggaa aatgaagtag ctcctacttt gtctatttaa
      661 cgctataata acaagcgatt cgactgataa aagtggagtt gtaaccaaca attcgatttt
      721 tctcagtttt gtagtttaat aattttctac ggtgatcgat tctatgggat atcatttttt
      781 aacagaagga aatgggatta ttgcatgtta tgacatgtac ccaactcagt ttcattctat
      841 taaggacatg attaattatt tgcctgtctt aaagcagatg gggtttaatg ccttatggat
      901 taatcccatg caaatgcccg gtgacattag cggctttttt aaaacggata aaaataatgg
      961 tgtaaaaaca ggaaatgagg tcactagaag cttatatgcc atgagtcacc cgttattatt
     1021 taatcctcaa ttcagtttgg gctcccctga agaccccatg gggacaaccc aacgattaaa
     1081 ctcagaagct ttacagttat tcactcaaaa tgcaaggaac ctcggaatag ttccaatgtt
     1141 tgatctggtt ttaaatcatg tcgccattga ttctcccttg tgtcaggata agcctcattg
     1201 gttcaaagga gttcatgaag acttcaaaga tgttcgcggc ttcaattacg atgatgaaag
     1261 cataagaaaa gaaataataa ccgaattttg gcaaccttat atcaaacgtt acatgatcga
     1321 gtacggtttt gatggtgttc gagtcgatgc tgtaggttat gtacatcctg cagtaagaag
     1381 tgaaatatat gcctatattc actctcttgc tgctgaatat ggtaaaccaa agccggttat
     1441 ccttgatgaa gctttattta gtaaacgacc acttgcggat gaagtaaatt atttgaaatt
     1501 acccggcata ggcccaactc acatcactac agaagcttat aatgttgaat ttgatcttgc
     1561 cagcgctgat ctgccttatg aaataacatt ggaagagcag ttaaaggcga gtgtagtctt
     1621 ccaacaaaaa gatggaacat ggcgtgaaaa cacgaagggg ggctgcatta atttttgtgg
     1681 gaatcatgac tatcgctcat tggcgatgac tattttattt caaatggcca aaaaaagact
     1741 ggaatctgac gctttttata atgacttaat gtcttcctat caagacttgt actttaagtt
     1801 taacgccgag caagaaaaac cacatgaact caaggagcct ttgaaaacaa ccttacttta
     1861 ctcttatgtt gatcaaatca aaaaggagtt ggaagataat aaagatgaaa caacacaaga
     1921 atttaaaact ctggtattag aaaaactggc tttatccgct cttacagcca gtggcggatg
     1981 gtttttatta tctggtgatg aaacatgcga tatcactgca aaaacagtat ttcagagaaa
     2041 aggtgctgtt gataaatctt attacccaca acgtgaacat cgtattttta gtgaaaaacc
     2101 agttattgct cacaaaattt tagaaaaaat ggcagaagag aatttcttca tagaaaactc
     2161 ggaaaataag tggatgcagg aactttacag aagtttatca agttttcctg aaatgcaaaa
     2221 acgtctttta gtatctcata ttgataatat taaacaccaa ataaatgccg gcatagatca
     2281 tgtacaagaa aaattttcca ggctacttgc cagtgaaaat ttaaatatag gtttcacatc
     2341 tcaagatttt ttagagaatc caagaaccca cgaaaatgg
//
LOCUS       DACTJE010000262         2370 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.264, whole genome shotgun sequence.
ACCESSION   DACTJE010000262 DACTJE010000000
VERSION     DACTJE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.264"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            569..844
                     /locus_tag="I9760_002965"
     CDS             569..844
                     /locus_tag="I9760_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT5044702.1"
                     /translation="MTLKNPSRLHLLNEFESAPHSALFNQQTIAAVLSCSTQLLERNR
                     WAGGGVPYLKIGRKVLYRKSDVLNFLQQQKIYYSTSDEGQLQLVENA"
     gene            910..>2370
                     /locus_tag="I9760_002966"
     CDS             910..>2370
                     /locus_tag="I9760_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAT5044703.1"
                     /translation="MSQNHVIELANTLKQRPIVCDYTGGRFRLTNEGLTYIGIDRDGT
                     PLPPRWICAPLHIVAKTRDAQSGEWGRLLEWQDDDGITHQWAMPLALLQGDASDVRRE
                     LARLGLSISPHRTARDLLASYLQVFPVDARARCVDKLGWHGDVFVTASQCIGQSTEKI
                     VFQNTNAIEPAMSIKGSVDKWRDSIGRLASGNSRLVFAISVALAPVLAKIVGEDSGGF
                     HFRGASSSGKSTALSLAASVWGNPQSYCRLWRSTTNGLEGLAALHNDGLLILDELSQM
                     DPREAGEAAYLLANGQGKTRASRTGTVRQSSRWSLFFLSAGEESLTALMAKSGQRINA
                     GQEIRLADIEADAGCQMGIFETIHDQLSPASMALSLKKYSSGYHGAIGRAWLNQVVAN
                     RQTISRFITDTIQSFVDIVIQPDATGQIIRVARRFALVAAAGELASQFGLTGWKKGES
                     FHAAKTCFIAWQDAFGVDGHREDRAIMAQVRAFFESH"
BASE COUNT          646 a          468 c          572 g          684 t
ORIGIN      
        1 tctgagttcg tccaaaatgg attatatgtc cgtctaatat ggaatagagc ttcgtccaaa
       61 atggaatgca aatgaatttt ttcgattatt taatgctcat tggtagacgt ataaaaatat
      121 ctgggactaa tgggacacgt ggggcatcca tgtcattact gggctagaca tgtccctagc
      181 ttatttgaga actttgggac gtatgggaca cggataattc ctgcacttaa gtaataaaac
      241 taaaggttat gataaattcg cgattcgcga attgcgaatt tatgacatgt tatatttcat
      301 ttttagtatc aatcaatatc cttcgataac acatctttga aattgatctt ttgtgcttga
      361 cattctttga gggaatacga gatacttttt ccaagcttaa ggagtaagcc cgatgttcca
      421 acaaaatgta ggcagttgga agtcaaagga ttaggagaaa agaatatcaa tcaattgtcc
      481 agggtgatac tggatgatgc gttgagttct gttcaaggaa gatacatctc ggcatttggc
      541 agaggtgtaa ataactgagg aatttcctat gactttaaaa aatccatccc gtttacatct
      601 attaaacgaa tttgaatctg cgccacattc tgcgcttttt aatcaacaaa cgattgctgc
      661 agtattaagc tgctccaccc agttacttga acgtaaccgc tgggcgggtg gtggtgttcc
      721 ctatttaaaa atcggtcgta aggtgttgta tcgaaaaagc gatgtgttga actttctcca
      781 acaacaaaaa atttattact caaccagtga cgaaggtcag ttacagctcg ttgagaacgc
      841 ataaatttag aattagtcag aatgaattgg attcattccg atggttgact gttgtttgga
      901 gaataatgta tgtcacaaaa tcatgttatt gaattagcca atacactaaa gcagcgtcca
      961 attgtatgcg actataccgg tggtcgtttt cggttaacta atgaggggct aacttatatc
     1021 ggtattgata gggatggcac tcctttacca cctcggtgga tatgtgcacc attgcatatc
     1081 gttgccaaaa cccgagatgc tcagagcggt gagtggggtc gtttgctgga atggcaagat
     1141 gatgatggga taactcatca gtgggcgatg ccattggctt tattgcaggg tgatgcctcc
     1201 gatgtaaggc gagagctggc aagattagga ttgagtattt cacctcatag aactgctcgt
     1261 gatttgttag cctcctattt acaagtcttt ccagtagacg cacgtgcaag atgcgttgat
     1321 aaactgggtt ggcatggcga tgtctttgta accgcttccc aatgtatcgg gcaatcaaca
     1381 gagaagattg tcttccaaaa taccaatgcc attgaaccgg caatgtccat caaaggcagc
     1441 gttgataaat ggcgagattc aataggtcgt ctggcttccg gcaactcaag attggtattt
     1501 gccatctcgg tagcacttgc tccagttcta gcaaagattg ttggtgaaga ctcaggaggc
     1561 tttcatttca gaggtgcatc ctcatcaggt aaaagcactg cattgagtct ggctgcctct
     1621 gtttggggaa acccacaatc ttattgccgt ttatggcgca gcactaccaa tgggcttgaa
     1681 ggcttagccg cattgcataa tgacggcttg ctgattcttg atgaacttag tcaaatggat
     1741 cctagagaag caggggaggc agcttattta ctggccaatg gtcagggaaa aacacgtgcc
     1801 tctcgtactg gcacagtcag gcaatcttct cgatggtctt tatttttctt atctgctggt
     1861 gaagagtcct tgacggcact catggcaaaa tcaggacaac gcattaatgc aggtcaggaa
     1921 atcaggcttg ctgatattga ggcggatgct ggctgtcaga tggggatctt tgaaacgatt
     1981 catgatcagc tcagtccagc tagcatggcc ttatcgctca agaagtacag cagcggatat
     2041 catggcgcaa tcggcagggc gtggctaaat caagtggttg ctaaccggca aacaatcagt
     2101 cgattcatta ccgacaccat tcaatctttt gttgatatcg tcattcagcc tgatgcaaca
     2161 ggccagatta tccgtgttgc cagacgcttt gcactggttg ctgctgcggg agaattggcc
     2221 agtcaatttg gtctgacagg atggaaaaaa ggcgaatctt tccatgctgc gaagacttgt
     2281 ttcattgcat ggcaggatgc ttttggagtt gatggccacc gcgaagatcg cgccattatg
     2341 gcgcaggtac gcgctttctt tgaatcccat
//
LOCUS       DACTJE010000263         2333 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.265, whole genome shotgun sequence.
ACCESSION   DACTJE010000263 DACTJE010000000
VERSION     DACTJE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.265"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1232
                     /locus_tag="I9760_002967"
     CDS             <1..1232
                     /locus_tag="I9760_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lpg2382 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044704.1"
                     /translation="SLCILLAYIEENELIDDAEIKNASFTIQLQINLLCLLEAITVTM
                     ANSNDFAHDIYNHMMKQRGKGATGNPFYNFLFGIPSRATLFERLKLISIDPGLTSIIF
                     HRLMGTNPETQADLDDFVSQSRLSGWNADVELANFDVASTSTLPAMGVNILEAVWEDS
                     TGHNKNTSGILNAQAGLGLIGIMEEKEYTTSFKLAETTLPQGTRLHSDLGYSLIPELK
                     VDNSPKKVSQFHIDERWMNLYNSWNLCFVIANIDPVFVPLKLLIPSVFSAEPSNYKET
                     RVLTLFLVANLFLNQQTLNNPLFSNNFSFRNGDAIMKKWGEINKNHASELLQQCCAKE
                     YKEPDTLYNSILGEHPHLNFVLKLLSYFQDSHQFRLTKKAKQSTLSDYLFFSGNAASA
                     DNSEQLNLASQPSLNKV"
     gene            1471..>2333
                     /locus_tag="I9760_002968"
     CDS             1471..>2333
                     /locus_tag="I9760_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT5044705.1"
                     /translation="MNILSCLKGFIATVDCKSFSKAAQKLYISPSKLSKQITWLEEEL
                     KVTLLIRSTTRLIVTESGHRLYEKALDLFEQLEVVKVIATPDQFELNGIIRLYLTVTP
                     AIPYLTSLSIQFMRKYPGIEIDFIVGSDTKELYSSTFDLAISFDDVKHAKLVCKRLFS
                     IQRNVFASPDYITQYGLPQTIDELPKHNCLINTLYGLQNKWVFNKKIIHVSGNFKSNN
                     AGVLKQAAVAGLGLLLAPYFSVYEEIKNEALIQVLPHKSSPDINLYAIYPKSIMDEQK
                     INLLLNFFVKRP"
BASE COUNT          760 a          411 c          398 g          764 t
ORIGIN      
        1 aatctttatg tattttgctt gcctatattg aagaaaacga attaattgat gacgcagaaa
       61 tcaaaaatgc atcttttacg atacagctcc aaattaattt attatgcctt ttagaggcaa
      121 taaccgtcac tatggccaat agtaatgatt ttgctcatga tatttataac catatgatga
      181 agcaaagagg aaaaggcgct acaggtaatc ctttttacaa ttttttattt ggaatacctt
      241 ctcgtgccac cttatttgaa agattaaaac tgatttccat agatcctggt ttgacaagca
      301 ttatatttca tcgattgatg ggtacaaatc cggaaactca ggctgatctc gatgatttcg
      361 taagtcaaag tcgtttatct ggatggaatg ctgatgtcga actggctaat tttgatgttg
      421 catcaacgag cactttacct gctatgggag ttaacatttt agaagccgtg tgggaagact
      481 caacaggcca taataaaaat accagtggta ttttaaatgc acaagctggc ttgggattaa
      541 ttggtataat ggaagaaaaa gaatacacca cttcttttaa attggctgaa actactttgc
      601 ctcagggcac tagacttcat tcagatttag gttattcatt gatacctgag ttaaaagttg
      661 ataactcacc aaagaaagta tctcagtttc atatcgatga gcgatggatg aatttataca
      721 atagctggaa tttatgtttt gtgattgcaa atattgatcc tgtattcgta ccacttaaat
      781 tgttaatacc cagtgtgttt tcagcagaac catccaatta taaagaaaca agagtgctca
      841 ccttgtttct tgttgcgaat ctcttcttaa accaacaaac tcttaataac cctctatttt
      901 ccaataattt ttcctttcga aatggagatg ctattatgaa aaaatggggt gaaatcaata
      961 aaaatcatgc cagtgaacta ttgcaacaat gttgcgcgaa agaatacaaa gaacctgata
     1021 ctctatataa tagtatactt ggtgagcacc ctcacttaaa ctttgtgtta aaacttctct
     1081 cttattttca ggattcacat cagtttagac tcactaaaaa agcaaaacaa agtacactga
     1141 gtgattattt atttttctca ggtaatgccg catctgcgga taattcagag caactgaatt
     1201 tagcctctca accttctttg aataaggtat aaaaatttta tgctttcatc gcattgaata
     1261 ggtagttgga acctgagact ggttatagct agtctccgtt aaaaaaattt ccttttggaa
     1321 tcgctcatga ggtaatgtgg ctatgaattt taagctgctg ttttcaagat caaatcagct
     1381 ttctctgtca ggaaatcatt gttgttgatt tacaaaactg tcatccaggt ctataccatt
     1441 taaaaagagt tcaattaaga aaatttcaag atgaatattt tatcctgttt gaaaggcttt
     1501 attgcaaccg ttgattgcaa aagtttttca aaggccgcac aaaaattata tatatccccc
     1561 tctaaattaa gcaaacaaat aacttggctg gaggaggaat taaaagttac cttgttgata
     1621 cgctctacaa cacgattaat tgtgactgaa tcaggccata ggttgtatga aaaggcttta
     1681 gatcttttcg agcaattaga ggtagtcaag gtcattgcaa cgcccgatca gttcgaatta
     1741 aatggcataa tcaggctcta tcttactgtt actccggcaa ttccctactt aacctcatta
     1801 agtattcaat ttatgcgtaa atatcccggg attgaaatag attttattgt tggttcggat
     1861 acaaaagagc tctactcctc tacctttgat ctggctattt catttgatga tgtcaaacat
     1921 gcaaaactgg tttgcaaaag attattttca atccagagaa atgtctttgc ttcacctgat
     1981 tatatcactc aatatgggct tcctcagaca atcgacgaac taccaaagca taattgtttg
     2041 attaacactc tctatggcct gcaaaataaa tgggttttta acaaaaaaat cattcatgtc
     2101 tcgggaaatt ttaaaagcaa taatgcgggt gttttaaaac aagctgcggt tgcaggccta
     2161 ggacttctat tggctcctta tttttcagtt tatgaagaaa taaaaaatga agcattaatt
     2221 caggtactac cccataaatc ttcaccagat atcaatcttt atgccattta cccaaagtct
     2281 ataatggatg aacaaaaaat taatttactt ttgaactttt ttgtgaaaag gcc
//
LOCUS       DACTJE010000264         2314 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.266, whole genome shotgun sequence.
ACCESSION   DACTJE010000264 DACTJE010000000
VERSION     DACTJE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.266"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..830
                     /locus_tag="I9760_002969"
     CDS             <1..830
                     /locus_tag="I9760_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT5044706.1"
                     /translation="RVFLNWGLCDKKIYRELSSLNFDFSTVGFFPHVYGQSLLFYLIR
                     PLIKIHFFNKRLLDVGCGNGVGLKISSELLKTRYALGIDLVSKLVTNSNNSFYIEDKI
                     NYMQADAENMAIANESFDIVTNLESSHLYPQIEHFFSEVERVLAPNGFFCYADVHFDV
                     KQQAQRLEAFVKTKKNLRIIEKHNITKMVQASIYQRLIVNEKGFYQLAQYLLGPDKIH
                     YTSELANVASSMGLMFLPWWRIRFKNPALQALGKFARRCKHWNKKYYFYYLIQKIDS"
     gene            complement(943..1785)
                     /gene="htpX"
                     /locus_tag="I9760_002970"
     CDS             complement(943..1785)
                     /gene="htpX"
                     /locus_tag="I9760_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HAT5044707.1"
                     /translation="MINNLKTFILLASLTALLVVIGGLLGGSTGMLIALVFAGVMNFS
                     AYWYSDVLVLKMYNAEPLPNNHFVNNIISELAHRAGTPVPKVYLINNSTPNAFATGRN
                     PENASIAVTTGLLDRLTQEEITGVLAHELAHVIHRDTLINVVSATIAGAISGIANMFM
                     WLSMFGHNSNNEEGVHPVVGMIMMIVAPLAAGLIQMAISRSREFEADAGGARISGNPQ
                     WLASALLKLDQANHEQYFDEAETHPSTAHLFIINPLNGEKLANLFSTHPSTAERVARL
                     RAMY"
     gene            1999..>2314
                     /locus_tag="I9760_002971"
     CDS             1999..>2314
                     /locus_tag="I9760_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0181 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044708.1"
                     /translation="MYSKYPAFFLNKNIKSSSGVQFSNVVKIPSAIESLYRGDNNLTG
                     IIFLLPTLITGVFCQNFPEVVDIEQIRLHKLTNLSNDFHMVSMSEDPQIALDWGNGCF
                     ITI"
BASE COUNT          702 a          410 c          436 g          766 t
ORIGIN      
        1 acagagtttt tttgaactgg ggattatgtg ataaaaaaat atatcgcgaa ttatcgtcat
       61 tgaattttga tttttctaca gtaggttttt tccctcatgt ttatgggcaa tcacttcttt
      121 tttatttgat aaggccatta ataaaaatcc atttttttaa taaaagatta cttgatgtgg
      181 gttgtggtaa tggcgtaggt ttgaaaataa gttctgaatt attaaaaaca agatacgcgt
      241 taggcattga tttggtcagc aaattggtca ctaattccaa taatagcttc tatatagaag
      301 ataaaattaa ttatatgcag gctgatgcag aaaatatggc tattgcaaac gaaagttttg
      361 atattgtcac taaccttgaa agttcacatc tttatcctca aatcgagcat tttttttctg
      421 aagtagagag ggttttagca cctaacggat ttttttgtta tgcggatgtt cattttgatg
      481 tgaagcagca agcgcagcga ttagaagcat ttgtgaagac aaaaaaaaat ttacgcatca
      541 tagaaaagca taatattact aaaatggtgc aagcatcaat ttatcaacgc ttaattgtca
      601 atgaaaaagg gttttaccaa ttggctcaat accttttggg gcctgataag attcactata
      661 cttcggaact ggctaatgtg gctagttcaa tggggctcat gtttcttccc tggtggagga
      721 ttcgctttaa aaatccggca ttgcaagcgc tgggaaaatt tgctcgccga tgcaagcact
      781 ggaataaaaa atattatttt tattatttaa ttcaaaagat agactcataa aaaccaatct
      841 attatttcca gggcattatt ttattttggg tttgataaat tttaaaagtc attccttact
      901 gtcagtaaag taatgtttac tccaaatcag tcatcctgtt aattagtaca tggctcttag
      961 tcttgctact cgttctgctg tcgatggatg ggttgaaaat aagttagcca gcttttctcc
     1021 gtttaagggg ttaatgataa acaggtgcgc agtggagggg tgagtttctg cttcatcaaa
     1081 gtattgctca tggttggctt gatcaagttt taacaaggca ctggccagcc actgcggatt
     1141 cccggaaatt cgtgctccac cggcatccgc ttcaaactcc ctggagcgcg atatagccat
     1201 ttgaatcagt ccagccgcca ggggagctac tatcatcatg atcatgccaa ccactggatg
     1261 gacaccttct tcattatttg aattgtggcc aaacattgaa agccacatga acatattggc
     1321 aatcccgcta atcgcccctg ctatcgttgc gcttaccaca ttaattaaag tgtcgcggtg
     1381 gattacatgg gccagttcat gagctaaaac tccggtgatt tcctcttgag tgagcctatc
     1441 taacaatcct gttgtcacag cgatactggc attctctgga tttctccctg ttgcaaacgc
     1501 gtttggagta gagttattaa ttaaataaac tttgggtaca ggggttcctg ccctatgcgc
     1561 aagttctgaa ataatattgt taacaaagtg attattgggc agaggttcag cattatacat
     1621 tttcaaaaca agaacatcag agtaccagta agctgaaaaa ttcataactc ctgcaaatac
     1681 taatgcaatc agcattcctg tagagcctcc aagcaatccc ccaataacaa ctaacaatgc
     1741 tgttagagag gctaacaaga taaaagtttt caggttattt atcatttctc atcacctcat
     1801 ttactcttcc tatattttat atctcataaa taaggccaga tgttaaaaat tcaacttgga
     1861 accttgtctc tgtttattta tgcccatgct ttagagttgg aaatcaagaa aaacccattg
     1921 agagtaatac ggcgttattt ttattttgag gtcaatgtga tatattgttg atcaattccg
     1981 tatgtcatta aacattttat gtatagtaaa tatccagcat ttttcctaaa taagaatata
     2041 aaatcttcct caggtgtgca gttttcaaat gttgtaaaaa ttccctctgc aattgaatca
     2101 ttatatcgag gtgataataa tcttacaggt atcatttttc ttttgcccac tctaattaca
     2161 ggagtttttt gtcaaaattt tcctgaagtt gtggatatag agcaaataag attgcacaaa
     2221 ctgaccaacc tgtctaatga ttttcatatg gtcagtatgt cagaggatcc tcaaattgca
     2281 ttagattggg ggaatggatg ttttataaca atcg
//
LOCUS       DACTJE010000265         2219 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.267, whole genome shotgun sequence.
ACCESSION   DACTJE010000265 DACTJE010000000
VERSION     DACTJE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.267"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            10..1662
                     /gene="kaiC"
                     /locus_tag="I9760_002972"
     CDS             10..1662
                     /gene="kaiC"
                     /locus_tag="I9760_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HAT5044709.1"
                     /translation="MNTNKAKTGIKGLDDILNGGYLKNKPTLLKGSPGTGKTIFTLFF
                     THEQLKSNNSVIYVTCDESPEQIIAHMDGFNLESSKFLQEGKLLILDFTPILTDEVAG
                     EFNINALLLRIEQAQQKIKADTLIIDSLQSILLGIKNYDPNYELLRLFQWARQKNLTV
                     LTTMAETQTILQTEIYDEYVVDCAIELKQKVKNDLMTRYLRIIKNRGSSHGTNEYPFS
                     ITHNGISLLPITSTKLNTTTSAEYLSTGIKTLDSMLGSKGYQTGSQIIISGRSGTAKT
                     LFATSFAQSAVRQGRKVLFISFEESPNDLIRHVKSINIDLMKYITKKKLHINSRRSVE
                     MGLEDHIISIIELTEEKQFDVLILDPISAMLDLGNTMEVKRLFIRFISYMKSLNKTLL
                     FTELIPDYSKELTILGLSSLTETWIRLSNVESNGEFNRLIHIAKARGIKTSNQIKEFY
                     VTDKGINIEAPYLGDNQMMFGSQKSAHILREKQELQQRKEEIKRIELEIDKLAETQRA
                     QLKIQEATFLAKRTELIAKKNELIAKEEALIKRMESNKLLRE"
     gene            1667..1939
                     /locus_tag="I9760_002973"
     CDS             1667..1939
                     /locus_tag="I9760_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiB"
                     /protein_id="HAT5044710.1"
                     /translation="MIRTKLKLFVIGSSAISKRAIINLQSICSDPKLADLCDIEVVDL
                     CKNKGIAEQEKILATPILIKKEPLPERRIIGDLSDKQKVISALEMD"
     gene            1948..>2219
                     /locus_tag="I9760_002974"
     CDS             1948..>2219
                     /locus_tag="I9760_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HAT5044711.1"
                     /translation="MISNEHINTQNFWYQLVEKMAIGVLVINNEGQILYANDYAVQLF
                     GAIRTELIGTNFLYPMAADETQEVEILKSSNEILTAHMTVRKSIWE"
BASE COUNT          794 a          403 c          405 g          617 t
ORIGIN      
        1 ggacgaaatg tgaataccaa taaagcaaaa acgggaatta aggggttaga tgatatttta
       61 aatggtggtt atttaaaaaa taaaccaacc cttctcaaag gcagccctgg tacaggaaaa
      121 acaatattta cccttttttt tacgcatgaa caacttaagt caaacaacag cgtcatttat
      181 gtcacctgcg atgagtcacc tgagcaaatt attgctcata tggatggctt taacctggaa
      241 agcagcaaat ttttacaaga aggcaagctt ttaattcttg acttcacacc tatattaacg
      301 gacgaagtgg ccggggaatt caatatcaat gcacttttac tccgtataga acaggctcaa
      361 cagaaaatca aagccgatac actaattatt gattcacttc agtccattct tttaggcatc
      421 aaaaattatg atcccaacta cgagttgtta agattatttc agtgggcgcg gcaaaaaaat
      481 cttaccgttc tcacaacgat ggctgaaacc caaacgattt tacaaacaga aatctatgac
      541 gaatatgttg ttgattgtgc tattgagcta aaacaaaaag tcaagaacga cctgatgacg
      601 cgctatttgc gaatcattaa aaatcgtggt tcgtctcatg gcactaatga gtatcccttt
      661 tctattactc ataatggcat ttcattactt cccatcacta gcacaaaact gaatacaaca
      721 acttctgctg aatatctcag cactggtata aaaaccctgg atagtatgct aggcagtaaa
      781 ggataccaaa caggatcgca gataataatc agcggacgtt caggaacagc aaaaacgctt
      841 tttgcaacaa gtttcgccca atccgctgtt cgacaaggca ggaaagtgct gttcatctca
      901 tttgaagaat cccctaatga tttaatccgt catgtcaagt ccattaatat tgatttaatg
      961 aaatatatca ccaaaaaaaa attacatatt aattcaagac gctctgtgga aatgggactg
     1021 gaagaccata ttatttcaat catcgaatta acagaagaaa agcaatttga tgtccttata
     1081 ctggatccca tctcagctat gctggattta ggtaatacga tggaagtcaa gagactgttc
     1141 atacgtttta tttcttatat gaaatctctc aataaaaccc ttttatttac tgaactgata
     1201 cccgattatt ccaaagagct aaccatactg ggcttatcca gcttaacaga aacctggatt
     1261 cgcttatcta atgtggaaag taatggagaa ttcaatcgat taatccacat tgccaaggcg
     1321 cgtggcataa aaacatccaa ccaaatcaaa gaattttatg tcactgataa agggattaac
     1381 atagaagccc cttatcttgg tgataaccag atgatgtttg gttctcaaaa atcagcccac
     1441 atcttgagag aaaaacaaga attacaacag aggaaagaag aaattaaacg gattgagctt
     1501 gaaattgata agcttgcaga aacacaacgt gctcaactca agattcaaga ggcaaccttt
     1561 ttagctaaaa ggactgagtt gattgctaaa aaaaatgaat tgattgctaa agaagaagcg
     1621 cttattaaac gcatggagtc caacaaattg ttaagagagt aaatgaatga ttcgaactaa
     1681 gctaaaactg tttgtgattg gaagtagtgc tatatcaaaa cgtgccatta ttaatttgca
     1741 atcgatatgc tcagatccca agcttgctga tttgtgtgat atcgaggtag tcgatctatg
     1801 taaaaacaag gggatagcag aacaagaaaa aattctggca actccgatat taattaaaaa
     1861 agaaccatta cctgaacgtc gaatcatcgg tgatttatcc gataagcaaa aagttatttc
     1921 tgctttggaa atggattagg tgatatcgtg atatcaaacg agcatataaa cacacaaaac
     1981 ttttggtacc aactcgttga aaaaatggct attggggtat tggtgattaa taatgaaggg
     2041 caaattcttt atgccaatga ttatgccgtt caattatttg gcgccatccg tacagaactc
     2101 attggtacta atttcttata tcctatggct gcagatgaaa cacaggaagt tgaaatttta
     2161 aaatccagta atgaaatttt aacagcacat atgacagtca ggaaaagtat ctgggaaaa
//
LOCUS       DACTJE010000266         2160 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.268, whole genome shotgun sequence.
ACCESSION   DACTJE010000266 DACTJE010000000
VERSION     DACTJE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.268"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..1097
                     /locus_tag="I9760_002975"
     CDS             <1..1097
                     /locus_tag="I9760_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EbhA"
                     /protein_id="HAT5044712.1"
                     /translation="DLPEFITREDIFKLLSFKIFMPLAQIFAEREQWMRRIAGTNGKN
                     AHTSESYNKFKKIFLNSHHKFVIEESLFHFEHGTLKYHHEWQTAKRQIAKEVKSLRVL
                     QENWKEKKNKIDRKNASILFDFLDSFESIPNRLRSREEERFKQTEVYKKALSTDKLKA
                     IELLNQHLFDYSNPQEELAERMKLIKALYDSNILFEQHHTPIGWLKNQLNIETVLDKK
                     LASLEQSLKAAENALNSYMEHAMPKTVTISTELRKELEKDSIALPEENKSPEAVSENQ
                     NTSHSNPVVQPQITPVQNKKNTGKVYLKVQEYEENLHKLGLFTPPKKTFSQPLQIQKK
                     PLSDENKFSLKITNGADLKLFPTNSLHPQN"
     gene            1361..>2160
                     /gene="legL5"
                     /locus_tag="I9760_002976"
     CDS             1361..>2160
                     /gene="legL5"
                     /locus_tag="I9760_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL5"
                     /protein_id="HAT5044713.1"
                     /translation="MFQIGEHNMILIYKLTLTKLTEDELKKDFATIPSWATHLDLSNN
                     RLGETSNEELIRVFAKIPGWISHIKLRFNNLGQKNGKDLAKAFAKIPISVTTLDLGSN
                     QLGKKSGEELANAFAGIPTSVTRLILSQNELYKRTGGELATAFAKIPSSVTHLVLVNN
                     GLGEKNGKELATAFANIPSSVTTLDLALNDLSKIKSTELAVIFTKIPTSVTQLDLSHN
                     NLNKKSGKNLAKIFANIPTSVTNLNLSCNELGKKSGKGLAAAFAKIPTS"
BASE COUNT          789 a          413 c          364 g          594 t
ORIGIN      
        1 ctgatttgcc ggaattcatc accagggaag atattttcaa actcttaagc tttaaaatat
       61 ttatgccatt ggctcagata tttgcagaga gagagcaatg gatgagacgt attgcgggta
      121 ccaatggaaa aaatgctcat accagcgaaa gctacaataa attcaaaaaa atctttttaa
      181 actcccatca taaatttgtt attgaagaaa gcttattcca ttttgaacac ggcacattaa
      241 aatatcacca tgagtggcaa actgcgaagc gacaaattgc caaggaagta aaatcattac
      301 gtgtattaca agaaaattgg aaagagaaaa aaaacaaaat tgacagaaaa aatgcttcta
      361 tcctgtttga ttttcttgat agttttgaaa gtattcccaa ccggttacgc tcaagggaag
      421 aggaaagatt taaacaaact gaagtctaca aaaaagcact ttccacagac aagctcaaag
      481 caatagaact cctgaatcag catctttttg attacagcaa tcctcaagaa gaattggcag
      541 aaaggatgaa gcttatcaaa gctctgtatg acagcaatat tctatttgaa cagcaccata
      601 ctccaatagg gtggctgaaa aaccaattga acattgaaac ggtacttgat aaaaagctgg
      661 cttctctgga gcaatcctta aaagcagcgg aaaatgctct aaattcttat atggaacatg
      721 caatgcctaa aacggtcacg atttccactg aactaagaaa agagctggaa aaggatagca
      781 tcgcgctccc tgaggaaaat aaatcacctg aagcagtttc agaaaatcag aatacaagtc
      841 attctaatcc tgtagttcaa cctcagatta ctcctgttca aaataaaaaa aatacaggaa
      901 aagtttacct taaggtgcaa gaatacgaag aaaacctcca taaacttggt ttatttacac
      961 cccccaaaaa aaccttctct caaccattac aaatacaaaa aaagcctctt tctgatgaaa
     1021 ataaattctc gctaaaaata acaaatggag cagatttaaa gcttttcccc acaaattcat
     1081 tacaccccca gaattgattt tatttttgtc aaaaagtatt tttaaatatc tatgtatatt
     1141 ttttatacat cattgtgata tattattatc ccagtgattg tcttctccga atggtataac
     1201 aggagaactg gttttcctag ttttaatgat taaacgggtt ttcgctaaga tgaggtttgc
     1261 aaaagcaaac ttatgcttat cttagtgata tccatatgat tcgccttaat aactctcctc
     1321 atctttttga attttttatt catttaatag ccttattagg ctgtttcaaa taggagagca
     1381 caatatgata ctaatttata agcttactct gacaaaactt acagaggatg aattaaaaaa
     1441 agattttgcc acaattccaa gctgggcaac tcaccttgac ttatcaaaca atcgcctcgg
     1501 cgaaacaagc aatgaagaat taataagagt ctttgccaaa attccaggct ggataagcca
     1561 tattaaatta agatttaaca acctcggcca aaaaaacggt aaggacttgg caaaagcttt
     1621 tgccaaaata ccaatttcgg tgactacgct tgatttaggt tccaatcaac ttggcaaaaa
     1681 aagtggagag gaattggcaa atgcattcgc aggaattcca acatcggtga ccaggcttat
     1741 attgagccaa aacgaacttt acaagagaac tgggggggaa ctggcaactg catttgccaa
     1801 aatcccgagc tcagtaaccc atcttgtttt agttaataat ggattgggtg aaaaaaatgg
     1861 caaggaattg gcaactgctt tcgctaacat tccaagctca gtgaccaccc ttgatttagc
     1921 tcttaatgat ctttccaaaa taaaaagcac ggaattggca gtaatcttta caaaaattcc
     1981 aacttctgta actcaacttg atctgagcca caataacctc aataaaaaaa gtgggaagaa
     2041 cttagcaaaa atttttgcca acattcctac ctccgtaact aaccttaatt taagttgcaa
     2101 tgaattgggc aaaaaaagtg gcaaggggtt agcagctgcc tttgccaaaa ttccaacctc
//
LOCUS       DACTJE010000267         2138 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.269, whole genome shotgun sequence.
ACCESSION   DACTJE010000267 DACTJE010000000
VERSION     DACTJE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.269"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..994)
                     /locus_tag="I9760_002977"
     CDS             complement(<1..994)
                     /locus_tag="I9760_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT5044714.1"
                     /translation="MPWQECTKVDEKIKFVARLLDGEQMSSLCQEFGISRKTGHKIYN
                     RYKESGLEGLNDRSRKPHRYANQLPFQLEKEILKVKKEKPTWGAPKIREKILRQYPDV
                     KSPAISTIHTILDKYGLVTKRKRRRYKAEGTKLTNGKTPNELWCADYKGEFQLGSKEY
                     CYPLTLTDFNSRYLLACEGLSTTKEQYAITVFERVFKEYGLPNAIRTDNGVPFSSVQA
                     LFGLSKLSVWWLRLGISIERIRPGNPQENGRHERMHLTLKKETTKPSGENFLQQQEKF
                     DRFIDEYNNERPHQALDMRYPGEVYIPSNKEYKGLPEVDYPFHDKMITVTHCGRL"
     gene            1153..>2138
                     /locus_tag="I9760_002978"
     CDS             1153..>2138
                     /locus_tag="I9760_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISLpn4 family transposase"
                     /protein_id="HAT5044715.1"
                     /translation="MDLAIEDSAAWSEAIFGSVDLGDKRLTHRLAQIGKQLTSLPGGS
                     LPESCEGQDALIEGSYRFLRNKRVTASQIAEGGYQVTSWLSQRIPTLLAIEDTTTLSY
                     THQIKESLGDLGGPKEKSNRGFHVHTTMLMDAEQEKTIGLIAQERWCRDIKERGKKNH
                     RRVRLYTEKESYKWEKNTRELERRLGSKMSDVISVCDREADIFEYIQYKLDNHQRFIV
                     RASHNRKLEGSYGYLFQMLPSATILGTYTIAIAQKANRKKRQAALELKTASVTFSLPE
                     RRAKARELKPITLNVVIAKEKNPSESDCLEWVLLTTEATTTLECARKITRYY"
BASE COUNT          654 a          414 c          400 g          670 t
ORIGIN      
        1 ataacctacc acaatgagtt actgttatca ttttatcatg aaatggataa tcaacctcag
       61 gcagtccttt gtactccttg tttgatggga tatatacttc acctggataa cgcatatcta
      121 aagcttgatg aggacgttca ttattgtact catcaataaa tcggtcaaac ttctcttgct
      181 gctgtaagaa gttttctcct gagggttttg ttgtttcttt ctttaaagtt aaatgcatac
      241 gctcatgacg accattttct tgcggattgc ctggtcttat gcgctcaatg ctgataccta
      301 atcgcagcca ccatacggaa agtttactta gtccaaataa tgcttgtact gaagagaaag
      361 gaacaccatt atcggtacgg atggcgttag gtagcccata ttctttaaaa acacgttcaa
      421 atacagtgat agcatattgc tcttttgtcg ttgataaccc ttcgcatgca agtaagtagc
      481 gactgttaaa atcagtgagt gttaatgggt agcaatactc tttactccct aattgaaatt
      541 cacctttata atctgcacac cataattcgt tgggagtctt tccattagtt aacttagtac
      601 cttctgcttt gtaacgcctt ctcttacgtt ttgtgaccaa gccatattta tcaagaatag
      661 tatgaatggt gcttattgca ggtgatttta catctggata ttgtctcaat atcttttctc
      721 gtatcttggg agctccccaa gtaggtttct ctttctttac ctttaatatc tctttttcta
      781 attgaaaagg taattggttc gcataacgat gaggcttcct gcttcgatca tttaatcctt
      841 ccaatccact ttctttataa cgattgtata tcttatgccc cgtctttcga gaaataccga
      901 attcttggca taaagatgac atttgttcgc catctaacaa tcttgctaca aattttatct
      961 tttcatccac tttagtacac tcctgccatg gcatttctct tctccagaaa tgcctttgag
     1021 tgtaacctat gtctccagaa tattttgtta cctatgtctc aataaggaca tctttcccaa
     1081 atacctgaat cttctacaca tcttgtctat atcaaagaaa catgatcaaa tcgcctcatt
     1141 ttgaggtaat gtatggattt agcaattgag gattctgcgg catggtcgga agctattttt
     1201 ggttcagttg atttaggtga taaacgcctg actcatcggt tagcacagat aggtaaacaa
     1261 ctaacatcct tgcctggtgg ttctcttcca gaaagttgtg aaggacagga tgcactcata
     1321 gaagggagtt atcggttttt acgcaataaa cgagttactg cgagtcaaat tgcagagggt
     1381 ggttaccaag taacaagctg gttatctcag cgaatcccta ctcttttggc gattgaagac
     1441 acaaccacac tatcctatac ccatcaaata aaagaatcat taggtgattt aggcggtcct
     1501 aaagaaaaaa gcaatcgagg atttcatgtt cacaccacca tgctcatgga tgcagaacaa
     1561 gagaaaacaa taggattaat agcgcaagaa cgttggtgtc gagatatcaa agaaagaggc
     1621 aaaaaaaatc atcgacgagt aaggttgtat acagagaaag aaagttataa atgggaaaag
     1681 aatactcgag agttagagcg tcgtctgggt tctaaaatgt ctgatgtgat ttctgtttgc
     1741 gatagggaag ccgatatttt tgaatacatt cagtataagt tagataatca tcaacgtttt
     1801 attgttcgag cgagccataa tcgaaaactg gaaggaagtt atggttattt atttcaaatg
     1861 ttaccttctg caacaatttt gggtacttat acaattgcaa tagctcaaaa agcaaataga
     1921 aagaaacgcc aagcggctct tgaattaaaa acagcatccg ttactttctc actaccagag
     1981 cgaagagcca aagcacggga attaaagcca atcactttaa atgtggttat cgcaaaagag
     2041 aaaaatccat ctgaaagcga ttgccttgaa tgggttctat taacaacaga agcaaccaca
     2101 acattagagt gtgctcgaaa aataacacga tattatga
//
LOCUS       DACTJE010000268         2132 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.270, whole genome shotgun sequence.
ACCESSION   DACTJE010000268 DACTJE010000000
VERSION     DACTJE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.270"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..419)
                     /gene="mlaD"
                     /locus_tag="I9760_002979"
     CDS             complement(<1..419)
                     /gene="mlaD"
                     /locus_tag="I9760_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAT5044716.1"
                     /translation="MKKQRYVDISVGIFMLLGLLALLVMAMKVSNITDFMSDKTYRVT
                     ADFTDIGGLKVRAPVTVAGVRIGEVSRIELQPGELNARVTMTLNGDKKIPYEDASARI
                     LTEGLLGSNYISIVPGFDEDGDTDHPYLRDGDTIAKT"
     gene            complement(420..1202)
                     /gene="mlaE"
                     /locus_tag="I9760_002980"
     CDS             complement(420..1202)
                     /gene="mlaE"
                     /locus_tag="I9760_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAT5044717.1"
                     /translation="MLDTIARLGNRGVQALQGIGSSGLFLFSMLFRKPNVLRLWPLLR
                     YQLYFIGVLSCLIIVVSALFIGMVVGLQGYNTLQKFGASSQLGQLLALSVARELGPVI
                     SALLFAGRAGSALTAEIGLMKATEQLSSMDMMGVDPLGRVVYPRFVAGFIALPILALI
                     FSAVAIYGGYFIGVYWLGVDAGSFWSNMQAAVNFRIDILSGIIKSLVFAFVVTWISVY
                     QGFECVPTAEGISHATTKTVVYSSLAVLGLDFLLTAMMIGDW"
     gene            complement(1195..1992)
                     /locus_tag="I9760_002981"
     CDS             complement(1195..1992)
                     /locus_tag="I9760_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT5044718.1"
                     /translation="MPDNWVEIKNLFFSLDDHCIFDGIDMVVKQGKITAIMGPSGSGK
                     TTLLRLIGAQLRPDSGQIYVNGQNIHQLSRKSLYETRRNMGLLFQSSALFTHLSVFEN
                     VAFPLREHTELNASMIRNIVLMKLEAVGLRGAAHLMPAQLSGGMCRRVALARTIALDP
                     VLMMYDEPFTGQDPISMGVLVRLIQRLNQLLHTTTIIVSHDVEETCSIADYIYLISGG
                     KIIGQGTPDELKRSIEPQIRQFMHGEADGVVPFHYPAKPYTEELLNA"
BASE COUNT          712 a          487 c          335 g          598 t
ORIGIN      
        1 tgagtcttcg caatggtatc cccatcacgt aaatagggat gatctgtgtc accgtcctcg
       61 tcaaaaccgg gaacaatact aatataattc gaaccaagca agccttcggt caaaattcgt
      121 gcagaagcat cttcataagg aatcttttta tcaccattca aagtcatagt cactcttgca
      181 ttgagctcac ctggctgcaa ctcaattcga ctgacctcac caatacgaac acctgcaacg
      241 gtcactggag ctcgaacttt taaaccaccg atatcagtaa aatctgctgt gacacggtaa
      301 gttttatcag acataaaatc agtaatgtta ctcactttca tcgccattac taataatgct
      361 agcagcccaa gcaacataaa tattcctaca ctaatgtcta cataacgttg ctttttcatt
      421 taccaatctc caatcatcat tgcagtcaac aagaaatcaa gacctaatac agcaagcgat
      481 gagtatacta cagttttagt tgtagcatga ctgatgcctt cagctgttgg cacacactca
      541 aaaccttggt atacagaaat ccaggtaacc acaaatgcga aaaccaagct tttaataatc
      601 ccgcttaaga tatctatacg aaaatttaca gcagcttgca tattagacca aaaactccct
      661 gcatccacac ctaaccagta aacaccaata aaataaccgc catatatagc tactgcagaa
      721 aaaatcaaag caagtatcgg taacgctata aatcctgcga caaaacgcgg ataaaccact
      781 cttcctaatg gatccactcc catcatatcc atactggata actgctctgt tgctttcatt
      841 aaaccaattt cagctgtaag agccgacccg gcgcgccctg caaacaacag agcgctaatc
      901 acaggtccta attctctggc gacgcttaaa gccaataatt gacctaattg acttgaagca
      961 ccaaattttt gtaacgtatt atacccctga agtcctacaa ccataccaat aaacaaggct
     1021 gaaaccacaa taattaaaca agacaataca ccgataaaat aaagctgata acgtaataat
     1081 ggccataacc ttaaaacatt aggctttctg aacaacatgg aaaataaaaa cagccctgaa
     1141 ctaccaatac cttgtaaggc ttgtacacct cgattaccca gacgcgcgat tgtatcaagc
     1201 atttaataac tcctctgtat acggttttgc cggataatgg aaaggcacta ccccatccgc
     1261 ttctccatgc ataaactgtc ttatctgagg ctcaatagat cgctttaatt catccggagt
     1321 accttgacct ataattttac cccctgatat taagtagata taatctgcaa ttgaacaagt
     1381 ttcttcaaca tcatgagata cgataatagt agtggtatgc aataattgat tcaatcgttg
     1441 tattaatctg accaatactc ccatggaaat tggatcttgg ccggtaaaag gctcatcata
     1501 catcattaat acaggatcaa gagcaatggt tctggctaat gcaactctcc tgcacattcc
     1561 ccctgataat tgagcgggca ttagatgggc agctccacgc aaaccaaccg cttcaagttt
     1621 cattaataca atgtttctta tcatggacgc gtttaattca gtatgttctc ttaatggaaa
     1681 ggcaacattt tcaaatactg aaaggtgtgt aaataaggca ctactttgaa aaagtaatcc
     1741 catgtttcgc ctggtttcat aaagtgattt gcgggataat tgatgaatgt tctgaccatt
     1801 aacataaatt tggccactat caggccttaa ttgcgcacca attaaccgca gtaaagttgt
     1861 ctttccagaa ccactgggtc ccataatagc agtaatcttg ccttgcttaa ctaccatatc
     1921 aatgccatca aaaatgcagt gatcatcaag gctaaagaat agatttttta tttcaaccca
     1981 attatcaggc atgctttatt atccacttat tattttaacg ggtaatgatg tcagtttgat
     2041 atctatcaaa gacaggtaat gctttattat atctttaatt aagaagtata tactggataa
     2101 ataaaaatgt gtttaataat tgcgtttcca tt
//
LOCUS       DACTJE010000269         2091 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.271, whole genome shotgun sequence.
ACCESSION   DACTJE010000269 DACTJE010000000
VERSION     DACTJE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.271"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(40..1965)
                     /gene="ankK"
                     /locus_tag="I9760_002982"
     CDS             complement(40..1965)
                     /gene="ankK"
                     /locus_tag="I9760_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkK/LegA5"
                     /protein_id="HAT5044719.1"
                     /translation="MPRVYNLKDIYLGAPSFSGHEVYLDAVYYPSDSSEKNFRVIYKK
                     NKFGNANLSRMEVAFSQLARLFLDNGLTSFQKMVVNDANKVQGLIVEHLNYVIENKEG
                     LKQPFYTLNAPRNGCDYTEKRVTSSNEIPFYFLDKLPQGFFNQLLAAEKNNKLSIDYA
                     SLASILATSYTLEEDDLHKGNFGFYLVKKQGKPRVVFFKIDHDLMFVDSIMSFTTRRF
                     CHLFDGCDAFDITEEDLLKFPNLKYSANGYWPTKTSIFYKPWDNKDYRTYAEIQAFAD
                     LSHVEEFNKAKWRSFYKHILISQSQMEATLKACFDENNSSDRAHISLVIQAMLARQAR
                     LKAMLFSLKDFRDFILSQNGKERDLLCHEILNNLPEEERKSFENEIRQSLDYSHNLCC
                     SGLFEDGDTPLHIAIKSGDYRYDETIGMYGQFINTKNSSGKTPLDIALQMAGQSKVHP
                     ADVRKDYRFIMKHLLANGANQTKQFEEFDKIENIRSYQFHTPYLNKAIKAKTYHELKE
                     VLRDIGEDHQYCLKFKKMLAVECISEFIKANQDNLSLRGILLKLKKEVDGKGTKSENA
                     ALMYIRQLRSRLWIVRQIRGLYGWSTTQGEIDYMIDKELARLDTKDLKRLSLFDSRDS
                     STLDNVFLDISLSKNKI"
BASE COUNT          677 a          407 c          303 g          704 t
ORIGIN      
        1 atattgctaa acattggtat ttttctcaat agaatgaact tagattttat tctttgatag
       61 tgatatatcc aaaaagacat tatccaaagt actgctgtct ctggaatcaa ataatgaaag
      121 tctctttaaa tctttagtat ccaaacgcgc cagctcttta tcaatcatat aatcaatttc
      181 gccttgcgtt gtagaccagc cataaagtcc tctgatttga cgtacaatcc atagccgact
      241 tcttaattga cgtatataca ttaaagccgc attttctgat tttgttccct taccatccac
      301 ttcctttttt aattttaaca gtattcctct taaactgaga ttatcttgat tagcctttat
      361 aaattcagaa atgcattcaa ctgccaacat ctttttgaat ttcaaacaat actgatgatc
      421 ttcaccaatg tccctgagta cttctttcag ttcatgataa gttttagcct ttatggcttt
      481 gtttagataa ggagtatgaa attgatagga tctgatgttt tcaattttat cgaactcttc
      541 aaactgtttt gtctgattag ctccattcgc caataaatgt ttcatgataa aacgataatc
      601 tttacgcaca tccgcaggat gaactttaga ctgccctgcc atctgtaaag cgatatccaa
      661 aggagttttt cctgagctgt ttttcgtgtt gatgaattgc ccatacatgc caatcgtttc
      721 atcataacga taatcacctg acttaattgc tatatgcagc ggggtatccc catcctcaaa
      781 aagaccggaa caacagaggt tatggctata atctaacgat tgtctaattt cattttcaaa
      841 agattttctc tcttcctctg gcaagttatt caggatttca tgacataata aatctcgctc
      901 ttttccattt tgggaaagaa taaaatcacg aaaatctttt agagaaaata acatcgcctt
      961 taaacgcgcc tgtctggcta acatcgcttg aattaccaga gaaatatgtg ccctgtctga
     1021 agagttattc tcatcaaagc aggcctttaa ggtagcttcc atttgagatt gagagattaa
     1081 aatatgcttg taaaaagacc gccattttgc cttgttaaac tcttccacat gggataaatc
     1141 cgcaaaagcc tgaatttcag cataggtacg ataatcttta ttatcccatg gtttataaaa
     1201 aatgctggtt ttggttggcc aataaccatt agcagaatat tttaaattag gaaattttaa
     1261 caagtcttct tctgtgatat caaaagcatc acatccatca aacaaatggc agaatcttct
     1321 tgttgtaaat gacatgatgc tgtctacaaa cattaaatca tgatcaattt tgaaaaaaac
     1381 aactcgtggt tttccctgtt tcttcaccaa ataaaaacca aaattccctt tatgcaaatc
     1441 gtcttcttcc aaagtgtacg aagtagccaa tatacttgcc aaagaggcat aatctatgct
     1501 caatttatta tttttttctg ccgccagtaa ttgattaaaa aaaccttgag gcaacttatc
     1561 caggaaatag aaaggtatct cattcgagct ggtcactcgc ttttcagtat aatcacatcc
     1621 attccttggt gcattcaaag tataaaatgg ctgcttcaat ccttctttat tttcaatcac
     1681 ataatttaaa tgctcaacta tcaatccctg tactttattt gcatcattca ctaccatttt
     1741 ttgaaaggaa gttaatccat tatctaaaaa taatcgggct aactgactaa aagccacttc
     1801 catgcgagat aagttagcgt taccaaactt gtttttctta taaataaccc tgaaattttt
     1861 ttcggacgaa tcagaaggat aataaaccgc atcaagataa acttcatgcc ctgaaaaaga
     1921 aggggcaccc agataaatat ctttaagatt ataaactcta ggcatggact ggaagaaaaa
     1981 ttaacaaatt agattataat tttatcacaa tgtttcattc aaaacaatgt tcaaaattga
     2041 ttcataatga atcaaacatt atcaatatca tcgggttgta tttgtaaatt c
//
LOCUS       DACTJE010000270         2024 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.272, whole genome shotgun sequence.
ACCESSION   DACTJE010000270 DACTJE010000000
VERSION     DACTJE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.272"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..>2024
                     /gene="glnD"
                     /locus_tag="I9760_002983"
     CDS             <1..>2024
                     /gene="glnD"
                     /locus_tag="I9760_002983"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HAT5044720.1"
                     /translation="ELIYQTHTLHMWPSHQYFFAKLQEQQNRYAKYGETAYNLEPNIK
                     NGPGGLRDLQILLSISKRHFKIKKLAEGIGYGFITDKEYEELKYCQNFLWRVRFALHM
                     LAGKPEERLSFDYQVKLAQFFGYQDQSHILAIEQFMKDYFKVIKRNRELNEMLLQWFN
                     ETIVYHQKQKIIRLDDEFQLSNRFIEVRNNRVFKQNPQSILKLFYWLVKRPDIEGVRA
                     STIRLIRESLFLMGKRFRQSKETANIFINIFRTGNDPYDALQRMNRYGVLAHYLDCFA
                     TVTGQMQYDLFHAYTVDQHTLFVIRNISRFKKNEYAKQFPLCAKIITALEKPEILYLG
                     ALFHDIAKGRGGDHSELGAIEAQQFTQRHYMEAEDSKLIVWLVRYHLLMSQTAQRKDI
                     YDPKTIEQFCQLLPHARYLDYLYLLTVADICGTNPTLWNAWKDSLLKELYHAAKTRLH
                     KQQELLDEAALISIRKQYAMDILISDGISFRVIQDLWGQFKGKYFLHESPEVIARHTK
                     AILNSKQFPVVIIMPHHSQGGTEVFIYMPHKDERFTITTSVLSNHHVTIQEAAIITCD
                     NQFDLDTYIILDENNQAFLNEQRARDIQKSLCDHLANTGRLPAVSRRRLSRALTHFNV
                     KTQINFIDDNTNHQTQLFLVTNDRPGLLATISRVFLTLNIHLHNAKIATA"
BASE COUNT          693 a          384 c          351 g          596 t
ORIGIN      
        1 aggaattaat ataccaaaca cataccttgc acatgtggcc tagccatcaa tacttctttg
       61 ccaaacttca ggaacagcaa aatcgttatg caaagtatgg agaaaccgcc tacaaccttg
      121 aacctaatat taaaaatggt ccaggtggat taagagacct gcaaattttg ctaagcatca
      181 gcaaacgcca ttttaaaatt aaaaaattag ccgagggaat cggttacggc tttattactg
      241 ataaagagta tgaagaatta aaatactgcc agaactttct ttggcgcgtt cgatttgctt
      301 tgcatatgtt agcaggaaag cctgaggagc gcttgtcatt tgattatcag gttaaattag
      361 ctcaattttt tggatatcaa gatcagtctc atatcttggc catagagcaa tttatgaaag
      421 actactttaa agtcatcaaa cgcaatagag agctgaatga aatgctcttg caatggttca
      481 atgaaaccat agtctatcat caaaagcaaa aaattattcg tctggatgat gaatttcaac
      541 tgtcaaaccg attcattgaa gtacgcaata accgtgtatt taaacaaaat ccgcaatcta
      601 tattaaaact attctattgg cttgttaaac gccctgacat agaaggagta agggcaagca
      661 caatcagact aataagagaa tcattatttt taatgggcaa acgctttaga caatcaaaag
      721 aaaccgctaa tatatttatc aacatattca gaactggtaa tgatccatac gatgcattac
      781 aaagaatgaa tagatatggt gttctagcgc actatcttga ttgttttgct acagtcactg
      841 gacaaatgca gtatgattta ttccatgcct atactgttga tcagcataca ctgtttgtga
      901 tccgtaatat atctcgcttt aaaaagaacg aatatgctaa acaatttcct ttatgcgcca
      961 aaatcattac tgctttagag aaacctgaaa ttttgtatct cggcgcttta ttccatgaca
     1021 ttgccaaagg tcggggagga gatcattctg aattaggagc aattgaggca caacaattta
     1081 cacaacgcca ctatatggag gcagaagata gcaaattgat tgtttggctt gttcgctacc
     1141 atttactcat gtcacaaact gctcagcgta aagatattta tgatcctaaa acaattgagc
     1201 aattctgcca acttctgcct catgcaaggt atttggatta tctttattta ctcacagtag
     1261 cagatatttg cggtacaaat cctactcttt ggaatgcctg gaaagattca ttacttaaag
     1321 aattatatca tgcagcaaaa acaagacttc ataaacaaca agagttgctt gatgaagcag
     1381 ctctgatatc cattcgtaaa caatacgcta tggatatatt aatttcagat gggatatcct
     1441 tcagagtcat acaggattta tggggtcaat ttaagggaaa atattttctt catgaatctc
     1501 ctgaggtaat agccagacac accaaggcca tactgaacag caaacaattt ccagtggtaa
     1561 taattatgcc gcatcacagt cagggtggaa ctgaagtatt tatctacatg ccacataaag
     1621 atgagagatt tactatcacc acttcagtat tgagcaatca tcacgttact atacaggaag
     1681 cagcaattat tacttgtgat aatcaatttg atctggatac ttatattatt ttagatgaaa
     1741 acaatcaggc ttttttgaat gaacaacgtg ccagagacat ccaaaaaagt ctatgtgatc
     1801 atctggccaa tacaggacgc ctcccagctg tttctcgcag aagattatcc agagcattga
     1861 ctcattttaa tgtcaaaaca caaattaact ttattgatga caatacgaat catcaaaccc
     1921 aactatttct cgttaccaat gacagacctg gacttttggc gactataagt cgcgtgtttc
     1981 ttacactaaa cattcattta cataatgcaa aaattgctac agct
//
LOCUS       DACTJE010000271         2013 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.273, whole genome shotgun sequence.
ACCESSION   DACTJE010000271 DACTJE010000000
VERSION     DACTJE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.273"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..477
                     /locus_tag="I9760_002984"
     CDS             <1..477
                     /locus_tag="I9760_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAT5044721.1"
                     /translation="SPIFNVMTSESANSDYLYNAMVKTYERDYIHYQLGTDRLERFID
                     EFNNHQTKVVLVLMPLYQPLMRKVHDKLNVPQNQLIVNKIKQFAEKKKIAFVNLFEEL
                     QDPENFQDPVHMNVLGASKVTQLLAKKILNKDYFYPEGYLDREIYDYMKKPHEDKS"
     gene            474..1916
                     /locus_tag="I9760_002985"
     CDS             474..1916
                     /locus_tag="I9760_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HAT5044722.1"
                     /translation="MNFATFQFILFLSLSLLVFFFINAHYRKYFILICSYVFYGTWSI
                     PLISIILFSTSVDYYLGNRIHNTTSNQLKKLFLTLSIFINCSILIYFKYANFFLDNAN
                     TLGALIGIDLPIPKAVNIILPLGVSFYTFEAISYIVDIYRGEKPASNWLNYNFYIMYF
                     PHLISGPIVRYKEIYQQYEGTIELPEFSRINEGLRLVLYGFIFKLVIADGVASTVDSI
                     FNNYQNATHLESWLAAIAFTVQIYFDFLGYTHIARGVSLFFNIKLPLNFNHPYNAVNI
                     SDFWQRWHISLSLWIRDYLYIPFGGSRGTLIKTVNNLIATMFIAGLWHGASWNYAIWG
                     AYHGMLLSIYHIYRNKFKNKNIALANSYHRIASIAFTFLCVVVGWVIFRANSFDQAYT
                     LIAKMFNPIGIAKEIATHHASTNYTDSLFGLMALCALCFMGPQIKKFETQTLLSLQPL
                     WIKKCGYIFGMLMLVIFMFSPTKPFIYFVF"
BASE COUNT          617 a          367 c          328 g          701 t
ORIGIN      
        1 tcacctattt ttaatgtcat gacttctgag tctgctaact ctgattattt atacaatgca
       61 atggtgaaaa cttatgaaag agattacatt cattatcagt taggtacaga caggctagag
      121 cgttttatag atgaattcaa taaccatcaa actaaagtag ttcttgttct aatgccctta
      181 taccaaccat taatgcgtaa agttcatgac aaactcaatg taccccaaaa tcagttaatc
      241 gttaacaaaa ttaaacaatt tgcagagaag aaaaaaattg cttttgtcaa tttattcgaa
      301 gaattacaag acccggagaa ttttcaagat cccgtccata tgaatgttct cggcgcatca
      361 aaggtaaccc aactcttggc aaaaaaaatc ttaaacaaag attatttcta tccggaaggt
      421 tatctggata gagaaatata tgattacatg aaaaaacctc atgaggataa atcatgaact
      481 ttgcaacttt tcaatttatt ttatttttaa gcctctcatt gcttgtcttc ttttttatca
      541 atgcccatta tcgaaaatat tttattttga tttgcagtta tgtgttttat ggcacgtgga
      601 gtattccgct gattagtatc atacttttct ctacttctgt agattattat ttaggaaaca
      661 gaattcataa tacgacgtct aaccaattaa agaagctgtt tcttactcta tctattttta
      721 ttaattgctc gattttgatc tatttcaaat atgccaactt ttttttggac aacgcgaaca
      781 cgctaggtgc tcttattggt atcgatttac caatacccaa agcggttaac atcattctac
      841 ccctaggtgt ttctttttat acttttgaag caattagtta cattgtagat atctacagag
      901 gagaaaaacc agcgagcaac tggttgaatt ataattttta cattatgtat ttcccccacc
      961 tgatttcagg gcctattgtc cggtataagg aaatttatca acaatatgaa ggcactattg
     1021 aactaccaga gttctcccga attaatgagg gattaagact ggttttgtat ggttttattt
     1081 ttaaattggt catagcagat ggagtcgcct ctactgtaga ttccatattt aacaattacc
     1141 aaaacgcaac ccatcttgag tcatggcttg ctgccattgc gtttaccgta caaatttatt
     1201 ttgatttcct gggttatacc catattgcaa gaggagtttc cttatttttc aatataaaac
     1261 ttccgcttaa tttcaatcac ccatacaatg ccgtcaatat cagtgatttt tggcaacgat
     1321 ggcatatcag tctctcactc tggattagag attatttata cattcctttt ggtggtagtc
     1381 gaggaacttt aattaaaaca gttaataact taatagccac aatgttcatt gctgggttat
     1441 ggcacggagc ctcctggaat tatgctatct ggggagctta tcacgggatg ttgttgtcta
     1501 tataccacat ctatcgcaac aaatttaaaa acaaaaatat tgctctggca aacagctatc
     1561 accgaatagc atctattgct tttacatttt tatgtgttgt tgttggatgg gttattttta
     1621 gagctaattc ttttgaccaa gcttacacgt tgattgcaaa aatgtttaac ccgatcggca
     1681 ttgcaaaaga aattgcgact catcatgcct caaccaacta cactgattca ttatttggtt
     1741 taatggcatt atgtgcattg tgcttcatgg gaccacagat aaagaaattt gaaactcaaa
     1801 ctcttttatc acttcagcca ctatggatta aaaaatgtgg ttacatcttt ggtatgttga
     1861 tgctcgtgat attcatgttt tcaccaacaa aaccttttat ttactttgtc ttctagggcg
     1921 ttagacaatc aagactaatg tcttgataat atgtaaccat agtgaaatac cggagttgcg
     1981 attgatatac tgctcatgct gagttacaca tcc
//
LOCUS       DACTJE010000272         1893 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.274, whole genome shotgun sequence.
ACCESSION   DACTJE010000272 DACTJE010000000
VERSION     DACTJE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.274"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1281)
                     /locus_tag="I9760_002986"
     CDS             complement(<1..1281)
                     /locus_tag="I9760_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1449 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044723.1"
                     /translation="MKEKMILDKSNIINAIFSNLSADEIRKVKGVSHAFYDRATEYLT
                     GHLENENFHLNRVIPPSDAIQREQKIEFCAKSLQALICSISEGEEGKSENLHKFNNMM
                     EKIRSAVQSNLGLSRVQRETYETFHPIYVKLGAPNFPINDEGELDPGSIVYRTDHVKG
                     RDERKSSWLVLLKDDSNEDGLSICFDTKFRKYLLHIDGAGHVTSQLTLRWNAIYDDLL
                     PDTVMQQPLRSFTPWQRHAEANFFNGRSTNKILGLLTKNYPEIFNYDPTSYFKEEPVF
                     SVELNRNAPKKLSTDKKNIYLTLDESFFKTPNGFENGLNRQPILAVIKTNSSLRGDCF
                     SVVPAIYEKQKLHILIKGHQQQTKVVDVFHANFKTGELLSWELLDDVLIFGEPSTEYK
                     PALTDLNLLSDQQSELMSHVSTAPMVRGSGPGARQ"
     gene            complement(1666..>1893)
                     /locus_tag="I9760_002987"
     CDS             complement(1666..>1893)
                     /locus_tag="I9760_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5044724.1"
                     /translation="CQSNKINKELKRSLLSPRQKDCAKLLLQGMSYKEIGKILQLSAR
                     TVETHVNQLKTKLGCDNKAELILQLNGIITR"
BASE COUNT          577 a          350 c          308 g          658 t
ORIGIN      
        1 ctgtcgggcg ccaggcccgc ttcctcttac cattggtgcc gttgaaacat gagacattaa
       61 ctcggattgt tgatcggata aaagatttaa atcagttaat gcaggtttgt actctgtcga
      121 gggttctccg aaaattaaaa catcgtcaag caactcccat gacaggagtt ctcctgtttt
      181 aaaatttgca tgaaaaacat caactacctt agtttgctgt tgatgtccct taattagaat
      241 atgaagcttc tgtttttcgt aaatggctgg taccacagaa aaacaatctc ctctgagtga
      301 ggagtttgtt tttatcacag ccaatattgg ctgtctgttt aaaccattct caaaaccatt
      361 tggcgtttta aaaaaagatt catctaaagt taagtaaata ttctttttat ctgttgatag
      421 cttttttgga gcatttctat ttaattcaac actaaagaca ggttcctctt taaaataaga
      481 ggtaggatca tagttgaata tttccgggta attttttgtt aataacccca atattttatt
      541 ggttgaacgt ccattaaaaa aatttgcctc agcatgccgc tgccagggag taaaagatct
      601 aagtggttgc tgcataacag tgtcaggcaa taaatcgtca taaatagcat tccatctaag
      661 tgttaattga gaggtaacat gacctgcacc atctatgtgt agtagatatt tacgaaattt
      721 agtatcaaaa caaatggaaa gtccgtcttc atttgagtca tctttcaaca atactagcca
      781 tgatgatttt ctttcatctc tccccttaac atgatcagtt cgataaacaa tgctgcctgg
      841 atcaagttcc ccctcgtcat taattggaaa attgggcgct ccgagtttga cataaatagg
      901 atggaaagtt tcatatgttt ctcgttgaac acgacttaaa cccaggttag attgtacagc
      961 actgcgtatt ttttccatca tattattaaa tttatgcaaa ttctcagact ttccctcttc
     1021 tccttcagat atactacaaa taagtgcttg caaggattta gcacaaaatt caattttttg
     1081 ctctctttgt atcgcatcag aaggtggaat aactctgtta aggtgaaaat tttcattctc
     1141 gagatgacct gttaaatact cagtggcccg gtcataaaaa gcatgcgata cgcctttgac
     1201 tttcctgatt tcatctgccg aaagattact aaaaatagca tttataatat ttgacttgtc
     1261 taatatcatt ttttctttca tttacaacat tctctttttg acgcgttatc gcaatttcaa
     1321 ttcaccttca attttttctg cacccaatga tcagtcttga aataatattt tcaaaatttt
     1381 ataaattatg ggcatagcaa agaaaaaact caggccttgc cgtattcgaa tatcctctca
     1441 agccatattt acacacaaat gtgattaagc ttttttgttc ggggactatt tctactcttt
     1501 gataaagcct tacgtagaga cgagaagcaa tgctgctgtc caattagaaa aggaattggg
     1561 aatttaactt tattctgcat ttaagtactc tcttgtataa aaacataaag aatacttaaa
     1621 gtaacattca gagaatatcc gtaaaatcac ggataaaata gctatttatc ttgtaattat
     1681 tccgttcaat tgaagaatca attcagcttt attatcacat cccaattttg tttttaattg
     1741 attaacgtgg gtttcaacag ttctagctga taattggagt attttcccaa tttccttata
     1801 actcattcct tgcaataaga gttttgcaca gtccttttga cgaggagaaa gaagggatct
     1861 ttttaattcc ttattaattt tgttgctctg aca
//
LOCUS       DACTJE010000273         1791 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.275, whole genome shotgun sequence.
ACCESSION   DACTJE010000273 DACTJE010000000
VERSION     DACTJE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.275"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..457)
                     /locus_tag="I9760_002988"
     CDS             complement(<1..457)
                     /locus_tag="I9760_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HAT5044725.1"
                     /translation="MKTKQEVSQQDKLKDSKSSTPLQTKEPWFISDTLNITFDSYDFS
                     ISVTEQAPIPSRIVFSGGGSRILAHIGALDELTRHGLKFTEFSGSSAGAMVAAFAYLG
                     YNCSEIKQIISWFNEDKLLDSPLIFNFNNIKQIFNKGGLSSAKLMRQAAN"
     gene            complement(900..1718)
                     /locus_tag="I9760_002989"
     CDS             complement(900..1718)
                     /locus_tag="I9760_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HAT5044726.1"
                     /translation="MTNSNHLRNPVTKSLNDLCQDELSVIGSYLGAWNDLWSLTFTSS
                     EMKKSLIHQQTDELWKFFFKEFFPYIPLPTEDFYKNFMKNYKIWYQTLYRVKQDPFFL
                     MYLDKLKEDPAHLFRNDKEIIIAAIEKQPFLYKNFSRGYKEDEEVILCALKNNRFIIH
                     DVPRTVREGFKEIADIKEDKARVDACDKLIDKLSPQRVSKIKSLDLFKSQFTLQAQNI
                     TKKSGEQVNNRQLQRSTAPITPEGISSLVHLGLFRDTQIQYEEKEESLHNKYRK"
BASE COUNT          511 a          300 c          324 g          656 t
ORIGIN      
        1 aatttgctgc ttgacgcatt aattttgccg agctaagccc ccccttgtta aatatttgtt
       61 ttatgttatt gaaattaaaa atgagtgggc tatcaaggag tttatcttca ttaaaccagg
      121 aaataatttg ttttatttcg gaacaattat aaccaagata agcaaaggca gcgaccattg
      181 caccggctga gcttccggaa aattcagtga actttaatcc atgcctggtt aactcatcta
      241 gggcacctat gtgtgccagt attcttgaac ctccaccact gaaaacaata cgagagggta
      301 tgggggcttg ttcagtgaca gagatactaa aatcatagga gtcaaaagta atatttaagg
      361 tatcagagat gaaccatggt tcttttgtct gtagtggtgt agacgatttt gaatctttta
      421 atttatcctg ctgagaaact tcttgctttg ttttcatacc gataatccct tatataaaac
      481 aaattctaag ttatttttta cgattctttt gctacagcca tcacaatcta tcgagtattt
      541 aaaacgattt cgccctaagg tttttgtatt gagggtactc tcctttttta aatgattggt
      601 gtttcagtta ttgggtattt taaatcaaat tgagtaggtt aagatctcat ggatcaataa
      661 ttaacataaa tggtctttga gcagacgtca acttgtttca ccaattattc gaaatgaaaa
      721 tttaagttac tagacaactt gcagagagga gttatccgaa gggtgttttg tttaaaaaaa
      781 cacccattat tgcaatcaaa acatattagc ttccggcctt attcaatcta ttaaggctat
      841 gcttggctgt ttctgtaatg aatttgcgat agagcaaccc ttatcaaaat ttattcgcct
      901 cattttcgat atttgttgtg tagagattct tccttttcct catattggat ttgtgtgtct
      961 ctaaacaggc ctaaatgaac aagagagctg ataccttcgg gcgttatagg cgcagttgat
     1021 ctttgaagct ggcggttatt tacttgttct ccactttttt ttgttatatt ttgtgcttgc
     1081 agagtaaatt ggcttttaaa caagtcgagg cttttgattt tagaaacacg ttgtggtgaa
     1141 agcttgtcaa ttagcttgtc acacgcgtca actcttgctt tatcttcttt gatatcagct
     1201 atttctttga atccttctct cactgttctt ggtacatcat ggattatgaa tcgattattt
     1261 ttaagagcac acagaataac ctcttcgtct tctttgtatc ctcgggaaaa atttttatac
     1321 aaaaagggtt gtttctcaat ggctgctata ataatttctt tatcattcct aaataagtgt
     1381 gcaggatctt ctttgagttt atccagatac attagaaaaa aaggatcctg tttcacacgg
     1441 tacaatgttt gataccatat tttatagttt ttcatgaagt ttttataaaa atcctctgtt
     1501 ggtagaggaa tataagggaa aaattccttg aaaaaaaatt tccacaactc atcggtttgc
     1561 tgatggatta aggatttttt catttctgat gatgtaaatg ttaagctcca taaatcattc
     1621 caggcaccaa ggtaggagcc aattacagac agctcatctt ggcaaaggtc atttaatgat
     1681 ttggtaacag gattccttaa atggttactg tttgtcattt tttacctcct gattgtggtt
     1741 ttgtttttct atcatgtttt tttgatatgc aaatgtgact tcaagatgac t
//
LOCUS       DACTJE010000274         1768 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.276, whole genome shotgun sequence.
ACCESSION   DACTJE010000274 DACTJE010000000
VERSION     DACTJE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.276"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(23..640)
                     /gene="petA"
                     /locus_tag="I9760_002990"
     CDS             complement(23..640)
                     /gene="petA"
                     /locus_tag="I9760_002990"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HAT5044727.1"
                     /translation="MTDLNQDVSNHNQDEQLDEERRRFLLHTTCVLGGVGAACALTPF
                     ITSWLPSSKAQAAGAPVKVDVSRMEPGEQAIIEWRGKPVWIIKRSKDMLNQLQKEDES
                     QLRDPDSLVDQQPEYAQNKFRSINPEFLVLIGICTHLGCSPKYKPSLGELGPDWPGGF
                     FCPCHGSMFDLSGRVFKGVPAPINLEVPPYHFLDEHTIVIGEDAK"
     gene            complement(814..1245)
                     /gene="rpsI"
                     /locus_tag="I9760_002991"
     CDS             complement(814..1245)
                     /gene="rpsI"
                     /locus_tag="I9760_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAT5044728.1"
                     /translation="MAEMKQYYGTGRRKSSIARVFLRPGKGDIKVNDRTLQEYFCRET
                     SCMIVMQPLETVDLLNKFDVYITVTGGGISGQAGAVRLGIARALVAYDEDGLAEGAEP
                     SPDSFRRKLRARGLLTRDSRRVERKKVGLHKARRATQYSKR"
     gene            complement(1251..1685)
                     /gene="rplM"
                     /locus_tag="I9760_002992"
     CDS             complement(1251..1685)
                     /gene="rplM"
                     /locus_tag="I9760_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAT5044729.1"
                     /translation="MKTFSAKGNEVKRDWFVVDASEKVLGRLATEIARRLRGKHKAEY
                     TPHVDTGDYIIVTNAEKVIVTGRKFKNKMYHHHTGFPGGIKSASFEKLQDKNPTKIIE
                     LAVKGMLPKNPLGREMYRKLKVYAGSEHPHTAQQPKQLEIEE"
BASE COUNT          506 a          421 c          320 g          521 t
ORIGIN      
        1 taacccattc atgccttaat ccttattttg catcctcacc tatcacaatg gtgtgttcat
       61 ctaaaaagtg ataaggtggt acttccaaat tgataggggc aggaacgccc ttaaatactc
      121 tgcctgaaag atcaaacatt gacccatgac atgggcaaaa aaagccacct ggccaatcgg
      181 ggcctaactc accaagacta ggtttgtatt ttggagagca acccaaatga gtacaaatcc
      241 cgattaaaac caaaaactcg ggattaatgg agcgaaactt gttttgcgca tactccggct
      301 gttgatccac caaagaatcg ggatccctca gttgagactc atcttctttt tgtaactggt
      361 taagcatgtc cttgcttcgt ttaattatcc atactggttt gccacgccat tcaataatag
      421 cctgttcacc aggctccatt ctactgacat caactttgac tggagcacct gctgcttgtg
      481 ctttagagct tggtaaccag gaagtaataa aaggagttaa ggcgcatgca gcgcctaccc
      541 cgcctaagac acaggtagta tgcaacaaaa aacgacgccg ctcttcatcc aattgttcgt
      601 cttgattatg attacttaca tcctgattga gatcagtcat ttcgctcact gcttaatcat
      661 cctgattatg gtctatgaac tggagccagt tcgcctaata taactcccgt tagttataac
      721 aaagaataac aatttttcaa acggttggat aacttatttc ttagaaaata aaaaagcctc
      781 gtataaaacg aggcttggaa aaaagtaata gaattagcgc ttggagtact gagtagctcg
      841 gcgtgctttg tgcagcccca ctttctttcg ttccacacgt cttgaatcgc gagttaataa
      901 cccacgcgct cttagcttgc gacggaacga atcgggactt ggctcagcgc cttcggccaa
      961 accatcttca tcataagcaa ccaatgctct ggctattccc aaacgaacag ctcctgcttg
     1021 gccagatata ccaccaccag ttacagtaat gtagacatca aacttattaa gaagatctac
     1081 tgtttccaaa ggttgcatta caatcatgca tgatgtttct cgacagaaat attcctgcaa
     1141 agttctgtcg tttactttaa tatcaccttt cccaggacgt aaaaatacac gtgcaattga
     1201 actttttcta cggccggtgc cataatattg cttcatttca gccattatac ttattcctca
     1261 atctcaagtt gcttcggttg ctgcgcagta tgtggatgtt cgctgccagc atatactttc
     1321 aacttgcgat acatctctct acctaacggg tttttgggca acatgccttt tacagcaagc
     1381 tcaataattt ttgttggatt tttatcctgt aatttttcaa aggatgccga tttgatccct
     1441 ccaggaaacc ctgtatgatg atgatacatt ttgtttttaa acttgcgacc agtcacaata
     1501 accttttctg cattagtgac tataatgtaa tcgcctgtat caacatgggg agtatactca
     1561 gctttatgct tgccacgtaa acgacgagca atctctgtag ctaagcgacc caaaactttt
     1621 tcactggcat cgacaacaaa ccagtcacgc ttgacttcat tgccttttgc actaaatgtc
     1681 ttcattaaat aaactccgaa ccgcctcaat tggtaatctt tctatcatgg acgggcgatt
     1741 ttaaccaaaa cagaaacaac ctacaagt
//
LOCUS       DACTJE010000275         1758 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.277, whole genome shotgun sequence.
ACCESSION   DACTJE010000275 DACTJE010000000
VERSION     DACTJE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.277"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            154..381
                     /locus_tag="I9760_002993"
     CDS             154..381
                     /locus_tag="I9760_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT5044730.1"
                     /translation="MPKHEIANLIHYYRKQSGLSQQELARLAGVGKTVIYDIEKGKES
                     VRLNTLLKVLDVLNIQIKFETPFPQTTDNNS"
     gene            378..686
                     /locus_tag="I9760_002994"
     CDS             378..686
                     /locus_tag="I9760_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin HipA"
                     /protein_id="HAT5044731.1"
                     /translation="MRKAYVSVSGIKAGILEELQGGTYQFTYFEDYHGAPVSLTMPLK
                     NKVYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKNDYFGQLILVGQDVVGAVTIEEIR
                     "
     gene            683..1621
                     /locus_tag="I9760_002995"
     CDS             683..1621
                     /locus_tag="I9760_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2370 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044732.1"
                     /translation="MKHCPITYEKISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQ
                     EAIARVGKMSVQGVQKKLSAKLKIKEGCFEIVDQYGQYILKPQSDIYPELPENEAITM
                     TLAKTIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
                     SMEKVIAVIEQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAY
                     DLLNSTIAQKNTKEELALPLKGKKNNLTKSDFLKYFAIEKLGLNQNVIDGIVQEFHQV
                     IPKWQELIGFSFLSQEMQEKYLELLEQRCKRLNFFD"
BASE COUNT          623 a          292 c          311 g          532 t
ORIGIN      
        1 ataataaata aatatgggaa aataaggaaa tcaatcaaat ttaccgccat aacagtaatt
       61 tatggtgaat taatgccaag aaaagcaata ataaatttat ttttatcatt tttactgcta
      121 taacagtaat atataattaa tttttggtgt tccatgccca aacacgaaat tgccaattta
      181 atccattatt accgcaaaca aagcggctta tcacaacaag aattagcccg gttagcgggg
      241 gtcggtaaga cggtcatcta tgatattgaa aaagggaaag aatcggtgcg gctaaataca
      301 ttgctaaaag tgctcgatgt tttaaatatc caaataaaat ttgaaacgcc ttttcctcaa
      361 acaacggata ataattcatg agaaaagcat acgtatcggt gagtggcatt aaggctggaa
      421 tattagaaga attacaaggt ggaacctatc aatttaccta ctttgaggat tatcacggcg
      481 cacctgtttc actcactatg ccgttgaaaa ataaggtcta tgattttgac gtgttccccc
      541 ctttttttga aggattactt cctgaaggca ttatgcttga agcactatta cgcaaataca
      601 agatagataa aaatgattat tttggtcaat tgatactagt tggccaagat gtcgtaggcg
      661 cagtgactat tgaggaaata agatgaaaca ctgccctatt acttatgaaa agattagtga
      721 ccaggaaaac tattcgcaac gtggattgca tttgctttct cctcaattaa aaaaccttag
      781 tccattggat ttaagtgctg atgagcagcg tcaggaagca attgctcgag taggaaagat
      841 gtctgttcaa ggcgtccaaa agaaactaag cgcaaaacta aagattaaag aaggctgttt
      901 tgaaatcgtg gatcaatatg gtcagtatat tttaaaacca caaagtgata tttatccaga
      961 attgcctgaa aatgaagcca ttactatgac ccttgcaaaa actataggcc tcgaagttcc
     1021 ggttcatggt ttggtttatt ctaaagacaa cagtttaacc tacttcatta aacgctttga
     1081 tagaataggc cataataaaa agttagcttt agaagatttt gcacagctat caggtgaaga
     1141 tcgacataca aaatataaaa gttctatgga aaaagtaatt gcagtcatag aacagttttg
     1201 tacattccca aaaattgaat tcgtgaaatt atttaagttg acgttgttta acttcttggt
     1261 cggtaatgaa gacatgcatc taaaaaactt ttccttaatt acgaaggata gaaaaatttc
     1321 catatcacca gcctacgatt tacttaactc aaccatcgct caaaaaaaca caaaagaaga
     1381 actggcttta cctctaaaag gaaaaaaaaa taatttaaca aagagtgatt ttcttaaata
     1441 ctttgccatt gaaaaattag gattaaacca gaacgtcatc gatgggatcg tacaagagtt
     1501 ccatcaggta ataccaaaat ggcaagagct gattggcttc agttttttat ctcaagagat
     1561 gcaggaaaaa tatctcgaat tgctagagca acgatgcaag cgattaaatt tttttgatta
     1621 actaatatcc gcagtctcaa tcatatgctt aagtataaaa ttagctattt gttacatggg
     1681 ttttcttaat cgctcaacat ctttcatgat atactctgct gtaacgtcat tgttcatttt
     1741 tgcgttctgt tcattttt
//
LOCUS       DACTJE010000276         1730 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.278, whole genome shotgun sequence.
ACCESSION   DACTJE010000276 DACTJE010000000
VERSION     DACTJE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.278"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            173..655
                     /locus_tag="I9760_002996"
     CDS             173..655
                     /locus_tag="I9760_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HAT5044733.1"
                     /translation="MKFRFRFYLGIVLLILAIVCPLLIPLIVQSNFSVLIKGFLSSML
                     VFGLPEILIVLAIALLGKEVYGLIEQKIKNILFKEKVSKTRYRLGLVFFAFPLVIGLL
                     EVFVKEITLAYGSYYYWVEIIWTTMFALSFFICGKQFWDKFKSLFIYDSYVVFEKNKK
                     "
     gene            complement(677..1672)
                     /locus_tag="I9760_002997"
     CDS             complement(677..1672)
                     /locus_tag="I9760_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAT5044734.1"
                     /translation="MDPITHGALGAACAQAALGKYDKRIPWQAGALAGMAPDLDVFIR
                     FSQEPLSLEQWHRYLTHSLMFIPIGGLIVALFLLCFSQYRQYWKITIGASLVGYATHC
                     LLDSLTSYGTVLFWPWSDSRISWDIISIIDPVFTLPLLLGTAWSVIYQNQKAVIASLF
                     FCILFLIFNTILHNRAVDGIHQYATTNNFKINHPRALPKLASSTYWRIIAKQQDCLII
                     AEAKTSLFYPTTIRLIRQALSFNESMIPFALSSGQKSDLATFHWFTDGFLIVANKNPL
                     ILADARYTIDDNPIFSLWGIEILPGQEHINKLSLPKINEYCESPDSERYKEDGLN"
BASE COUNT          526 a          282 c          355 g          567 t
ORIGIN      
        1 agagttttaa gcgtcataga gtccaattgt atggcgtcgg gtggtttata gggcgaaaac
       61 ggatgagcaa ggctttattt aaaattgcaa gaattaatta ttcattttgt agatagtcaa
      121 tcttcttatg gattactatt tgtaagtaag gtaaatagtg ggagtaacca tcttgaaatt
      181 tcggttccgt ttttatctgg gtattgttct tttaattttg gcgatagtat gtcctttgct
      241 gatcccatta attgtgcaat ccaatttctc agtgctgata aaggggtttc tatcttcaat
      301 gttagtgttt ggtcttccag aaattttgat agtcttagct atcgctttac ttggaaaaga
      361 ggtgtatggc cttatcgagc aaaaaattaa aaatatcctt tttaaggaaa aagtctcaaa
      421 gactcgttat cgtttaggtt tggttttctt tgcctttcca ttggttatag ggttgctgga
      481 ggtgtttgta aaagaaatta ccttagccta tggaagttat tattattggg tggaaattat
      541 ttggacaacg atgtttgctt taagcttttt tatttgcggg aaacaatttt gggataaatt
      601 taaatctcta tttatttatg attcttatgt tgtttttgaa aaaaataaaa aatgagctta
      661 ttaattataa ttgtttctag tttagtccgt cctccttata gcgttcgcta tctggcgatt
      721 cacaatattc attgatttta ggcaagctta atttattaat atgttcttgc cctggcaata
      781 tctctattcc ccatagggag aatatggggt tgtcatctat cgtgtatctc gcatctgcga
      841 ggattaaggg atttttattg gcaacaatta gaaatccatc agtaaaccag tgaaatgtag
      901 ccaaatcact tttttgtccg gatgacaaag caaaaggaat cattgattca ttaaagctta
      961 aagcctgccg aatcagtctg attgtggttg gataaaaaag agacgttttc gcttccgcga
     1021 taattaaaca atcttgttgc ttggctatga tgcgccaata agtagagctg gccaatttag
     1081 gcagggcacg aggatggttt attttgaagt tattagtcgt agcgtattgg tgaatgccat
     1141 ctacagcgcg attatgtaat atcgtattaa aaatcagaaa taaaatgcag aaaaacaggc
     1201 ttgcaattac tgccttttga ttttgataga tgactgacca ggctgttcct agcaatagag
     1261 gtaaggtaaa tacgggatca ataatagaaa taatatccca actaatacga ctatcgctcc
     1321 agggccaaaa caaaacggtg ccgtaagaag tcaatgaatc caataaacaa tgagttgcat
     1381 aaccaacgag agaggctcca atggttattt tccaatattg tctatattgt gaaaaacaca
     1441 atagaaacag tgcaactatc agacctccaa ttgggataaa cattaaggaa tgcgtaagat
     1501 aacggtgcca ttgctcaagg ctcaatggtt cttggctaaa acgaataaaa acatccaaat
     1561 ccggagccat ccctgctaag gcacctgctt gccaggggat tctcttgtca tattttccta
     1621 aagccgcttg tgcacaagcg gcacctaaag caccgtgagt tatcggatcc attttttact
     1681 taacaggctg ataaacaatg aaaatgcgct tcttctgaga aaggtaaggc
//
LOCUS       DACTJE010000277         1713 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.279, whole genome shotgun sequence.
ACCESSION   DACTJE010000277 DACTJE010000000
VERSION     DACTJE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.279"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            181..1662
                     /locus_tag="I9760_002998"
     CDS             181..1662
                     /locus_tag="I9760_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HAT5044735.1"
                     /translation="MSFQTDKEIQQKFFNYTLFVLVVLLICRLIANYFFPLFDTTESR
                     YAEIARKMLETGNWVTLQQDYGVPFWAKPPLSIWLSAFFMQLLGVNEFAVRLPGLLLS
                     IGVLWLVWGVAKKQSGSMAALFSLLTLAGSLYFFLDAGTVMTDPALLFCITLASVSFW
                     HAMVYENKIWSYAFFAGLGLGLLAKGPIAVVLVGMAVFFWVLLRNEWLNLWKRLPWVK
                     GTLLLLLIAVPWYVLAELRTPGFLNYFILGEHFHRFLTPGWSGDKYGIVHHVPKGTIW
                     LYATAGVFPWNIIAGGWLVKHGKMLPSLCQSGDGWLSYLTLWMITTLLFFTFASNIIY
                     PYVFPSLPAFALFFAEILNRLNLELKHLKWIFISSLLCGVLFLAVAFVFSVKPNVVPN
                     THKPIITAWLNQNPTAGSYLVYWGYKTDFSAEFYSEGQVKAVKNSNDLCKLISNRLEN
                     YLVINSKETQQIREGLLARFTLIDTFRVHRDTLILFYAPVLTC"
BASE COUNT          436 a          300 c          381 g          596 t
ORIGIN      
        1 atttaaaaat taataaactt ggcttgggtg tgaagatctc aaaaggaaaa gggaggttag
       61 ggaataattg gttttatgga atttattcct ggggacgcta tgtccgagat accacaggct
      121 cactccaatg cttcactaaa tgatactccg atttagttgt ttaaataaaa gaggtatatt
      181 ttgagttttc aaacagataa agagattcaa caaaaattct ttaattacac cttgtttgtg
      241 ttggttgttc tgctgatatg tcgcttgatt gccaattatt tttttccttt gtttgatacg
      301 accgaatccc gttacgctga aattgcacgc aaaatgctgg aaacagggaa ctgggtgact
      361 ttgcagcagg attatggtgt tccattttgg gctaaaccac ctttatcaat ctggttgtct
      421 gcttttttta tgcaactgtt gggtgttaat gaattcgcag ttcgcctgcc aggtcttttg
      481 ctgtctattg gtgttttatg gttggtttgg ggagtggcaa aaaaacagag tggctcaatg
      541 gctgcgctct tttccctatt aacactggca gggtcgcttt attttttcct tgatgcaggg
      601 actgtgatga ctgaccctgc cctacttttt tgtatcacac ttgcaagtgt ctcgttttgg
      661 catgccatgg tttatgaaaa caaaatatgg tcttatgcat ttttcgcagg attgggcctg
      721 ggtttattag caaaaggccc aattgctgtg gtactggtcg gaatggctgt atttttctgg
      781 gttctgttac gcaatgaatg gcttaattta tggaagagat tgccgtgggt taaaggcacg
      841 cttcttcttt tgttaattgc tgtgccctgg tatgttctgg ctgaattacg aactccggga
      901 tttttaaatt attttatcct gggagagcat tttcatcgat ttttaacccc tggctggtct
      961 ggtgataaat acgggatagt gcaccatgta cccaaaggaa cgatatggtt atatgctaca
     1021 gcaggcgtct ttccgtggaa tattatagca ggtggctggc tggtgaaaca tggaaaaatg
     1081 ttgccctctc tgtgccaaag tggagacgga tggttaagtt atttaactct ttggatgatt
     1141 actacgcttc tttttttcac ttttgccagc aatattatat acccctatgt gtttccttct
     1201 ctgcccgcat ttgctttgtt ttttgctgaa attttgaatc gtctgaattt ggaattgaaa
     1261 catttgaagt ggatctttat ctcctcatta ctctgtggag ttttattttt ggcagtggct
     1321 tttgtattta gtgtaaaacc aaatgttgtg cctaatacgc ataagccgat tattactgca
     1381 tggctaaatc agaacccaac agcaggcagt taccttgttt attggggata taagacggat
     1441 ttctctgctg aattttactc ggaaggtcaa gttaaagcgg ttaaaaattc gaacgattta
     1501 tgcaaattaa tatctaatcg gttggaaaac tatttggtta tcaacagtaa agaaactcag
     1561 cagattcgcg agggattact tgcaagattc acactcatcg acactttcag agtccatcgg
     1621 gatactttaa tattgttcta tgccccagtg ctaacttgtt gagcagtggt ttccaatttc
     1681 atctcgtagt aaagtaacag gacttacgaa aaa
//
LOCUS       DACTJE010000278         1671 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.280, whole genome shotgun sequence.
ACCESSION   DACTJE010000278 DACTJE010000000
VERSION     DACTJE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.280"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..538)
                     /locus_tag="I9760_002999"
     CDS             complement(<1..538)
                     /locus_tag="I9760_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HAT5044736.1"
                     /translation="MKINCIVFGLSGLDTAPIVRAMKKHPKFDKVIWFGVVDGADINT
                     FEAYKYQSDYDYSIPLPEEYISCMQEALGNFLVSSCRRNETVFNGKTYTLGFGSTYEY
                     LHHFSRYCYLIYDLIIKNQINLIIVDGVPHTSIDVLLSQAAKYFNIRTLFLYQHCIAN
                     RFMYFTDLINRKVDFSLYY"
     gene            complement(609..1664)
                     /gene="pseI"
                     /locus_tag="I9760_003000"
     CDS             complement(609..1664)
                     /gene="pseI"
                     /locus_tag="I9760_003000"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HAT5044737.1"
                     /translation="MNQGFNVEKHIIGHGHKPFIIAEMSANHNQSLERAMQIVEAAAQ
                     AGVHALKLQTYTADTLTIDERNNEFFISDKSSLWYGKSLYELYQSAYTPWEWHEPIFK
                     RCNELGLIAFSTPFDVTAVNFLETLNVPLYKIASFDIINHELIKAVAKTGKPVIISTG
                     LANLSEISEAVQTAQEAGCNNLVLLKCTSTYPASPLDSNILTIPSMHSIFKCPVGLSD
                     HTLGIGAAIASVALGACVIEKHFTLSRADGGVDAAFSLEPDEMTQLVHESKRAFDSLG
                     KVTYQPTDSEMASKRYRRSLYVVQDLKAGDKLTEENIRAIRPGLGLPPKYFLSLIGKK
                     INKETKRGTPLSWELID"
BASE COUNT          529 a          300 c          301 g          541 t
ORIGIN      
        1 aataatataa agaaaaatca actttcctgt tgattaagtc agtaaaatac ataaatcgat
       61 ttgcaatgca atgttgatat aaaaataagg ttcttatgtt aaaatatttg gccgcttgag
      121 aaagtaaaac gtctatgctg gtatgaggga caccatctac aatgataagg ttaatttgat
      181 ttttaataat taaatcataa attaaatagc aataacgaga aaaatgatga agatactcat
      241 aagttgatcc aaatccaagt gtatatgtct ttccattaaa gacagtttca tttcttctgc
      301 aagaactaac taaaaaattt cccaaggcct cttgcataca agagatgtat tcttcaggaa
      361 gtgggataga gtaatcataa tcagattgat atttataagc ctcaaaggta tttatatctg
      421 cgccatccac aaccccaaac caaatgactt tgtcaaattt tggatgcttt ttcatggctc
      481 gcacaattgg cgctgtatct aatccagaaa gtccaaatac aatacaatta attttcatgc
      541 tatacctttt aaatttcgtt gatgttagca cctaggggct tcagttgcaa caaatcctgg
      601 tttacgtttt aatcaattaa ctcccaagac aaaggagtac ctctcttggt ttctttatta
      661 atcttttttc ctattagaga tagaaaatat tttgggggta gcccaagacc tggtctaata
      721 gctctgatat tttcttctgt caacttatct cctgctttta gatcctgtac cacatacaga
      781 ctgcgtcgat agcgcttgga agccatctca ctgtcagttg gttggtaggt gacttttcca
      841 aggctgtcaa aagcacgttt tgattcatga accagttgag tcatttcatc aggttctaat
      901 gaaaatgcag cgtcaactcc tccatcagca cgtgataggg tgaaatgctt ttctataaca
      961 caagctccaa gggcaacact tgcaatcgct gcacctatac ctaaagtatg atcagacaag
     1021 ccaactggac atttaaatat cgaatgcatg ctgggaatgg tgagaatgtt gctatcaagt
     1081 ggagatgcag gataagtact agtgcatttt agtaaaacca gattattaca ccctgcttct
     1141 tgcgctgttt gtacagcttc tgaaatttca gataagtttg cgagaccagt tgaaataatg
     1201 acaggtttgc cggttttggc aactgcttta attaattcat ggttaattat atcaaatgat
     1261 gcaattttat agagaggtac gttgagtgtt tctaaaaaat taactgctgt gacatcaaat
     1321 ggtgtactaa atgcgattaa gcctagttca ttacagcgtt taaaaattgg ctcgtgccat
     1381 tcccaaggag tataagctga ttggtatagc tcataaagag attttccata ccataaactg
     1441 gatttatcag aaataaaaaa ttcattgttt ctttcgtcga tagttaatgt atctgcagta
     1501 taagtttgca actttagagc atgaacaccc gcctgagctg cagcttcaac tatttgcatg
     1561 gctctttcaa gggactgatt atggttagct gacatttcag cgatgataaa cggcttatgt
     1621 ccatggccaa tgatatgttt ttctacatta aatccttgat tcattataca t
//
LOCUS       DACTJE010000279         1636 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.281, whole genome shotgun sequence.
ACCESSION   DACTJE010000279 DACTJE010000000
VERSION     DACTJE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.281"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..281)
                     /gene="fumC"
                     /locus_tag="I9760_003001"
     CDS             complement(<1..281)
                     /gene="fumC"
                     /locus_tag="I9760_003001"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652206.1"
                     /note="class II family (does not require metal);
                     tetrameric enzyme; fumarase C; reversibly converts
                     (S)-malate to fumarate and water; functions in the TCA
                     cycle; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAT5044738.1"
                     /translation="MAKTRVETDSMGEIEVPSDKYWGAQTERSLHHFNIGKDIMPREV
                     THAFGILKKAAALTNLELGKLPKDKADLIVQAAEEVSKGLLDEHFPLHV"
     gene            451..1179
                     /locus_tag="I9760_003002"
     CDS             451..1179
                     /locus_tag="I9760_003002"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAT5044739.1"
                     /translation="MRTIRIYQPGEYQPGQLLELSPEAGQHVGVVLRMEQGEQLTLFN
                     GDNKEFTASIERVKKKQVFVRIASVLEVNRESPLKIHLAQAISKGERMEMVMQKSAEL
                     GVACITPLITERCQVKIDKEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQFV
                     REAKEHLKLILHPAFSKTWRDYPVQPPDVALIIGPEGGFSDEEIRLTSGHGFLPLSLG
                     PRVLRTETAAITALSVLQAAGGDL"
     gene            1392..>1636
                     /gene="trxA"
                     /locus_tag="I9760_003003"
     CDS             1392..>1636
                     /gene="trxA"
                     /locus_tag="I9760_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAT5044740.1"
                     /translation="MSDHIKTVTDASFEQDVINSSKPVLVDFWAEWCGPCRALTPILE
                     EVAATHSKDMTFAKINIDENPQAPAKFGVMSIPTLIL"
BASE COUNT          466 a          313 c          366 g          491 t
ORIGIN      
        1 cagacatgca agggaaaatg ctcatccaat agtcctttgc tcacttcttc tgctgcctga
       61 acaatcaaat ccgctttgtc ttttggcagt ttgcctaatt caagattcgt cagggcggct
      121 gcctttttca ggataccaaa ggcatgggtt acttcgcgtg gcataatgtc tttgcctata
      181 ttgaagtgat gcaatgaacg ttcggtttgg gcgccccaat atttatcaga cgggacttca
      241 atttcaccca tgctgtctgt ttctacacgt gttttagcca tgttatcaat tccttagcaa
      301 agttaatctg ttgattatgt ttatttatgt ctagctgctt tatcagcgag ttgggaaaga
      361 ataaaatcaa tcgccaaaat tctagcatag cagacgtaaa ttggtaattt ttgttatagt
      421 atcctttact aaaaccaagg ataattggct gtgagaacaa tacgtatcta tcaacctggc
      481 gaataccagc cggggcaact tctggaatta tcgcctgagg cggggcagca tgttggcgta
      541 gtcttgcgca tggagcaagg tgaacaactg acactgttta atggggacaa caaagagttt
      601 acagcatcta ttgagagggt caaaaaaaag caggtattcg ttagaattgc ctctgttctt
      661 gaggtgaaca gggaatcccc attaaagata catttggctc aagccatttc caagggcgag
      721 cggatggaaa tggtgatgca aaaatcggca gagttaggag tggcatgcat tacacctctc
      781 attaccgagc gttgccaggt taaaatagac aaagaaaaaa tggcaaaaaa aatgcaccaa
      841 tggcttaata ttatcattgg agcttgcgag caatgtgggc gtaatcaaat tccagagttg
      901 agacagccag tttatttgga tcaatttgtc agagaagcca aagaacatct caagctcatt
      961 ttgcatccgg catttagcaa gacatggcgc gattatcctg tccaacctcc tgatgttgcc
     1021 ttgattattg gcccggaagg tggtttcagt gatgaggaaa ttagattgac cagtggtcat
     1081 ggatttctgc ctttgtcact ggggccgcga gtattgcgta cggaaaccgc ggcaataact
     1141 gcgttaagtg ttttgcaagc tgccggaggt gatctataat agacttcttt ccaaatcggt
     1201 ataagctgcg tcttttgttt tatagctcta ttacaagtat ttctggagta ttatatgtaa
     1261 actactcttt ttgaaaatct tgtgtttgct ctaaaacaaa acacacagga tacacaggaa
     1321 agattttaga tcgaatagca taatctggtc ttggaccaga cctttatatt tctaataact
     1381 gaggtttatt tatgagtgat catattaaaa cagtaaccga cgcaagcttt gagcaggatg
     1441 taattaattc atccaagcct gttcttgttg atttttgggc tgagtggtgt ggaccatgcc
     1501 gtgcattgac acctattctg gaagaagtgg cagccactca tagtaaagat atgacgtttg
     1561 ctaaaatcaa tatcgatgaa aatcctcaag ctccagctaa atttggtgtg atgagtatcc
     1621 ccactttgat tctttt
//
LOCUS       DACTJE010000280         1610 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.282, whole genome shotgun sequence.
ACCESSION   DACTJE010000280 DACTJE010000000
VERSION     DACTJE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.282"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..722)
                     /gene="ffh"
                     /locus_tag="I9760_003004"
     CDS             complement(<1..722)
                     /gene="ffh"
                     /locus_tag="I9760_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAT5044741.1"
                     /translation="MFDNLTERLTRTFKNLRGQGRLTEDNIQHALREVRLSLIEADVA
                     LPVIKEFIEQVKQKSLGQEVLTSLNPDQAFIKIVHDELIHVMGNERVELDYKTQPPAV
                     FLMAGLQGSGKTTSTAKLARYLKETENKKVMMASVDVYRPAAIHQLKVLAEQIDVAFF
                     DSDPNEQPIKIAQKALESARKQYVDVLILDTAGRLHVDEDMMSEIKSLHKAVNPIETL
                     FVVDSMTGQDAANTAKAFHEALP"
     gene            complement(974..>1610)
                     /gene="legA7"
                     /locus_tag="I9760_003005"
     CDS             complement(974..>1610)
                     /gene="legA7"
                     /locus_tag="I9760_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegA7"
                     /protein_id="HAT5044742.1"
                     /translation="KKVNLSLYNDTLTVYPKSSVLPKPIQIRSELDLLMVLLFSQMDC
                     NSAQVDKFFMMRSNELGVYRFIHRDDTDHDLYHQDEFSMDDNHYHAQFDKIIDEIKLE
                     NILGILEKHSLISSEEHVSFIEAYHKANTLPEDMPKLETTLSSPLPVSSPSVHTTKKQ
                     PVVKEKEVDTPTTFAGLRVGFFLTSTKTTTKPLSRSHDDEKKTGMDGHGFH"
BASE COUNT          455 a          293 c          335 g          527 t
ORIGIN      
        1 gggagggctt catgaaatgc ttttgcagta ttggcagcgt cttgtcctgt catgctgtca
       61 acgacaaaaa gggtttcgat tggattgact gctttatgca aagacttgat ttcagacatc
      121 atgtcttcat caacgtgcag gcgccctgct gtatcgagaa tgagaacgtc aacatactgt
      181 tttctggcgc tttccaatgc cttttgagct atttttattg gttgctcatt agggtctgaa
      241 tcaaagaagg cgacgtcaat ttgttcggct aatactttca gttggtgtat ggcggctggt
      301 ctgtatacgt caacgcttgc catcatgact tttttatttt cggtttcttt caaataacgg
      361 gcgagttttg ctgtactcgt tgttttacca gaaccttgta agccggccat taaaaagaca
      421 gcaggcggtt gggttttgta atcaagttca acccgctcat tgcccatgac gtgaatgagt
      481 tcatcatgca cgattttaat gaatgcctga tcgggattta aactggttaa gacttcctgc
      541 ccaagagact tttgctttac ttgctcaata aactctttga taacaggcaa agccacgtct
      601 gcttcaataa gagacagtct tacttcacgc aaggcatgtt ggatattgtc ttccgtcagg
      661 cggccttggc ctcgcaaatt tttaaaggtt cgggttaagc gttcggttaa attatcaaac
      721 atagtgagta gtacccaaaa aatgactact ataacataga gagttaaaag tataccacag
      781 agaagagcgc attaatgatt agctctaatc gatagcagtg aatcaatcag ggttatcttt
      841 atgattttgt aattagaggc tgtagagtaa tttattagtt actctacagt ttttcaggtt
      901 ttggataaat caccataaat tgttaagtaa accaatagaa cgatcttcac tatggcttat
      961 caaactatat caattaatgg aacccatgcc catccattcc ggttttcttc tcatcatcgt
     1021 gagatctgga taaaggtttt gttgttgttt ttgttgaagt aaggaaaaat cctactctta
     1081 gccctgcgaa agtggttgga gtatctactt ctttttcttt cacaacaggt tgctttttag
     1141 ttgtgtgaac tgaaggagag gatacaggca aaggactgga cagagttgtt tccagttttg
     1201 gcatatcttc ggggagagta ttggctttat ggtaggcttc gataaagctt acgtgttctt
     1261 cagacgaaat aagagaatgc ttttctagta ttcccagaat attttccaat ttaatctcgt
     1321 caataatctt gtcaaattgt gcatgataat ggttatcgtc catgctaaat tcatcttgat
     1381 gatacaaatc atgatcagtg tcgtcacgat gtataaagcg atagacacca agttcattcg
     1441 aacgcatcat aaaaaattta tcaacctgtg cactattgca atccatttga gaaaacaaca
     1501 gaaccataag aagatcaagc tcactacgta tttgaatagg ttttggtagc acagatgatt
     1561 tagggtagac agtcaatgta tcgttatata aactgagatt gactttttta
//
LOCUS       DACTJE010000281         1605 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.283, whole genome shotgun sequence.
ACCESSION   DACTJE010000281 DACTJE010000000
VERSION     DACTJE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.283"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            275..451
                     /locus_tag="I9760_003006"
     CDS             275..451
                     /locus_tag="I9760_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HAT5044743.1"
                     /translation="MQEYKRYICVICGFIYDEAEGWPEDGIEPGTRWEDVPENWFCPD
                     CGAGKEDFEMVEMD"
     gene            complement(448..870)
                     /gene="hemJ"
                     /locus_tag="I9760_003007"
     CDS             complement(448..870)
                     /gene="hemJ"
                     /locus_tag="I9760_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HAT5044744.1"
                     /translation="MLFVKAFHIIALVAWFAGLFYLPRLYVYHAESLDKISIDRFKIM
                     ERRLYYGITWPAALATTFLGLWLSTYNLSYYLKAGWMHMKLALVLLLWVYHLTCGHFL
                     KSFALGKNTRSSRFYRFFNEMPTLLLVGIVLLVVVKPF"
     gene            complement(873..1604)
                     /locus_tag="I9760_003008"
     CDS             complement(873..1604)
                     /locus_tag="I9760_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAT5044745.1"
                     /translation="MDFIDIGLSLTALIILEVVLGIDNLVILSILTEKLPREKRKKAR
                     RWGLTFAWMTRLLLLGSAVFLVKLVKPILTIGSLVFSARDLFLILGGAFLIWKSTDEI
                     HQDVMNEPLMEPVSKNVSSSATFRGVVIQIALLDIIFSLDSVLTAVGLTSHFIVMAVA
                     ITIAIIIMIWASEPVSKFISEHPTIKMLALSYLILIGTVLVADGFEFHVPRGYLYFAM
                     GFSLAVESLNLIKNERVKRKKLKRE"
BASE COUNT          567 a          286 c          277 g          475 t
ORIGIN      
        1 actatctaat caaattagac tgataaccct gaggcaagaa aatattacaa tcatttcatg
       61 aatattgcaa aaaatagtgg cagcacctgt ctttaaaaga taaccacatt gactggtttt
      121 tggggttgat tttagcaaac ctatgtcaat tctcgacgaa gttataatgt tcattataag
      181 gtttaattta aaatttatta tgatattaaa aaggtttaac tttacataat catcaacagt
      241 agataaatta gcactttttt attgagttta gactatgcaa gagtacaaaa gatacatttg
      301 tgttatttgt ggttttattt atgatgaggc tgaaggttgg ccagaagatg gtattgaacc
      361 aggcacacga tgggaagatg ttcctgaaaa ctggttttgt cctgattgtg gtgcaggaaa
      421 agaagatttc gaaatggttg aaatggatta aaatggtttt acaaccacca acaaaacaat
      481 gcctaccagc agcagtgtag gcatttcatt aaaaaatcga taaaaacgag agcttcttgt
      541 attctttcca agggcgaatg atttaagaaa gtggccgcaa gttaaatgat atacccataa
      601 gagcaaaacc aatgcaagct tcatgtgcat ccaccctgct ttaagataat aagataaatt
      661 gtaagtggat agccacaacc caagaaatgt ggttgcaaga gctgcaggcc atgtaattcc
      721 gtaatataat cttctttcca ttattttaaa ccgatcaatg ctaattttat ctagtgattc
      781 agcgtggtat acgtagagcc gtggtaaata aaacaaccct gcgaaccagg ctaccaaggc
      841 gataatatga aaagctttaa caaataacat acttattctc tttttagttt tttacgcttt
      901 acacgttcat ttttaatcaa gttgagtgat tcaactgcta atgaaaaacc catagcaaaa
      961 tacaagtatc ccctgggcac atggaattca aaaccatcag ctaccaaaac ggttcctatt
     1021 aaaatcaaat aactaagcgc cagcatttta atggtaggat gttcgctaat aaatttactt
     1081 acaggctcac tcgcccaaat cataatgata attgcgatgg taattgctac tgccatgact
     1141 ataaaatgag atgttagccc tacggctgtg agaacgctat ctagtgaaaa tataatatca
     1201 agcaatgcga tttgaataac aacccccctg aaagtggcag atgaagaaac attttttgaa
     1261 acaggttcca ttaatggctc attcataacg tcctgatgaa tttcatcagt tgacttccaa
     1321 attaaaaaag caccacctaa tatgaggaac agatcacgag cagaaaatac cagactgcct
     1381 attgtcagaa ttggttttac taacttaact aaaaaaactg ctgagcctag taaaagaagt
     1441 cgtgtcatcc aggcaaatgt taagccccat cttctcgcct tttttctttt ttcacgtggt
     1501 aatttttctg taagaatcga tagaataacc agattatcta ttccaagaac aacttcaaga
     1561 ataatcaaag cagttaaact aaggcctata tcaataaaat ccatt
//
LOCUS       DACTJE010000282         1598 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.284, whole genome shotgun sequence.
ACCESSION   DACTJE010000282 DACTJE010000000
VERSION     DACTJE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.284"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(224..1513)
                     /gene="hemL"
                     /locus_tag="I9760_003009"
     CDS             complement(224..1513)
                     /gene="hemL"
                     /locus_tag="I9760_003009"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAT5044746.1"
                     /translation="MSRSSDLFHKAQTIIPGGVNSPVRAFKGVGGEPVFFKSGKGAYL
                     TDVDDKQYIDYVGSWGPLILGHCHPKVIEAVDNVLHRGMSFGAPTELEIQLAEKIASL
                     MPSIEKIRMVNSGTEATMTAIRLARGFTNKNKFIKFNGCYHGHSDSLLVKAGSGLLTL
                     GIPSTPGIPQSITEHTLTADFNNLEQVAQLFEKYPNDIATVILEPIPGNMGFILPKIE
                     FLKGLRELCDQYNALLIFDEVMTGFRVGLHGAQGLFGIKPDITTLGKIIGGGMPVGAL
                     GGKREIMSFLAPEGPVYQAGTLSGNPLAMAAGLATLKEIEKINFFEDLSNTTNKLTEA
                     LADAAENANIPFFAASLGGMFGFCFTDKNSVENYLDVASSDEVLFKKFFHAMLAQGVY
                     FAPSMYEAGFVSSMHGDLEIQKTYDAAELVLNQLKSA"
BASE COUNT          521 a          320 c          264 g          493 t
ORIGIN      
        1 ctgatttttc accaagattc cctacttgcg atctagagaa tggaatatga catagggaaa
       61 aacagctgtc tagtgtttta ttaattattt aaaattgcag ttaaccagat ttaaaaatct
      121 gttctgcaat cgcatttaga cgctttactg aacttaatta cccaaatact gattttaaaa
      181 attgtattta agtaacttaa ggtaataagg tacaattctg ctatcaggca gattttaatt
      241 gattcagtac caattcagca gcatcatatg ttttttgtat ttccaaatca ccatgcatac
      301 tggagacaaa tcccgcttca tacatagatg gcgcaaaata tactccttga gcaagcatag
      361 catgaaaaaa tttcttaaat aaaacttcat ccgaggaagc aacatccaaa taattttcaa
      421 cactattttt atctgtaaaa caaaacccga acattccgcc caatgatgca gcaaaaaatg
      481 gaatgtttgc gttttctgcc gcatcagcta atgcctcggt taatttatta gtcgtattgc
      541 ttaaatcttc aaaaaaatta attttttcaa tctcttttaa agtagccaaa cctgcggcca
      601 tagccaatgg atttcctgaa agagtaccag cctgatatac agggccttca ggcgccagaa
      661 aggacataat ttcacgttta ccacctaaag cacctacagg cataccacca cctatgattt
      721 ttccaagtgt tgtgatatca ggtttaatcc caaacaagcc ctgtgcacca tgtaaaccaa
      781 ctctaaatcc agtcattact tcatcaaaaa ttaacaaagc attatattga tcgcaaagct
      841 cgcgtaatcc ttttaaaaac tctatcttag gtaggataaa tcccatattg ccaggaatag
      901 gttccaaaat tacagtagcg atatcgtttg gatatttttc aaagagttga gcaacttgtt
      961 ccaggttatt aaaatcagcc gttaaagtat gttcggtaat gctttgtgga atgcctggag
     1021 ttgaaggaat tccgagagta agcaaaccag atcccgcttt aactaatagg ctatcgctat
     1081 gcccatgata acagccgtta aatttaataa atttattttt attggtgaat ccgcgcgcca
     1141 gccgaattgc agtcatagtt gcttcagttc ctgagttcac catgcgaatt ttttctattg
     1201 atggcatcag ggaagcaatt ttttcagcta attgtatttc cagttctgtt ggagcgccaa
     1261 agctcatacc acggtgtaat acattatcca cagcttctat caccttggga tggcagtgtc
     1321 cgaggataag tggtccccat gatccaacat aatcaatata ttgtttatca tctacatccg
     1381 tcaggtaggc acctttgcca gatttgaaaa aaacaggttc acctccaaca cctttgaaag
     1441 ctcgaactgg tgagttaaca cctccgggaa tgattgtttg tgctttatga aataaatctg
     1501 aagaacggct catagaaaat cccaaaaatg ataaacttta gacaaatatc agttattatt
     1561 tttaatattc aagatgtttc agaaatttat tatacaaa
//
LOCUS       DACTJE010000283         1588 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.285, whole genome shotgun sequence.
ACCESSION   DACTJE010000283 DACTJE010000000
VERSION     DACTJE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.285"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(185..1033)
                     /locus_tag="I9760_003010"
     CDS             complement(185..1033)
                     /locus_tag="I9760_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HAT5044747.1"
                     /translation="MTDLRTEIESLIHEGLLPDREYVSAKRFINHYATTSKFWDGLAL
                     VGITPIINGLYAGYYASRAVWATLRTVGNLLILKPGLAFDAIKDVGCHLTLSTCLLLM
                     APIHALTHAVELLTRVISSWFVGQESRYDLLETGLMAKWANRANGYQMVNELPSSNYF
                     NKARFFSSYNNAWDCLEQMASPITSLAKSGFHSLVEALYGVDSAIECIVNLAICKPLH
                     AKENFYNLQVHFGLALSLAVMAPINALVEGIAFMTRLGTTWVSACLKPEVTETEHENT
                     TAYQFN"
     gene            complement(1370..>1588)
                     /locus_tag="I9760_003011"
     CDS             complement(1370..>1588)
                     /locus_tag="I9760_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044748.1"
                     /translation="GLLFDGGFGVHNEDKWNKDTFDIEDMLEYDLQLLEKHSGGLNRT
                     YPRYCPDFSIDVITNLIHKHLELVEKNK"
BASE COUNT          522 a          300 c          297 g          469 t
ORIGIN      
        1 tgttttcatt ttcaatagtt acaaatcctt acctaaaaga caacaggcgg gattaattcg
       61 aaaggttatt cgaagaatgt actaatttta tggaattata caaggaaatc agtcctgaat
      121 cacaaagacc ttggtatttt ttaatgagct acagtcgcaa gcgactgtag caggcaggta
      181 agaattagtt gaattgataa gcggttgtat tctcatgctc agtctcagtc acttctggtt
      241 tgaggcaagc agaaacccag gtagtaccaa gacgtgtcat aaaggcaatt ccttcgacca
      301 gagcgttaat tggtgccatc acagccaaag agagagccaa accaaaatgt acttgtaagt
      361 tatagaaatt ctcttttgca tgtaaaggct tacagatagc caaattgaca atacactcta
      421 tggcagaatc aaccccatac aacgcttcga ctaatgaatg aaatccactt ttagccaagg
      481 atgttattgg ggatgccatt tgttctagac aatcccaagc attgttataa gaagaaaaga
      541 acctggcttt attgaagtaa ttggatgaag gcagctcatt aaccatttgg tatccatttg
      601 ctctgtttgc ccactttgcc ataagcccgg tttctaataa atcatagcgt gattcttgtc
      661 caacaaacca tgatgatata actctggtca gaagttcaac tgcatgagtc agagcgtgaa
      721 ttggagccat aagtagtaaa caagtagaga gagtcaaatg acaacccaca tctttgattg
      781 cgtcgaatgc caagcctggt tttaaaatta ataaattacc tacagtccgc aaggttgccc
      841 atactgccct tgaggcataa tagccagcat ataatccatt gataataggc gtaattccta
      901 ctaatgccaa accatcccag aatttgcttg tagtggcata gtgattaata aaacgcttgg
      961 ctgaaacata ttctctatct ggcaataatc cttcatgaat gagcgattca atttctgttc
     1021 taaggtctgt cattatggtc tctctctatg ttaattaaac aagcggggag agtataacta
     1081 aaaaaaacat aaatgaccaa tattggccca tgttgtattt gatgtttgca tctgttttcc
     1141 aaggtctttt gaacacagat caaaggccat agaatcaata atactcttat tgagtataaa
     1201 tcattgaata aattttcttt attatggcgt atgctccaat cggccatgat gcagcacata
     1261 ctgtgatgga aaatgcggta tgaaatacta caaagatgga gtggggcatg aagtacaagg
     1321 taagttttat cgaattaaaa acaataaata gactttgggt agcaaattgt cacttatttt
     1381 tttcaaccag ttccagatgt ttgtgtatta aattggtaat gacatcaatc gaaaaatcag
     1441 ggcaataccg gggatatgtc ctgtttaaac caccagaatg cttctctaaa agctgaaggt
     1501 catattccaa catatcttct atatcgaaag tgtctttatt ccatttgtct tcattatgaa
     1561 caccgaaacc tccatcaaaa agcaaccc
//
LOCUS       DACTJE010000284         1572 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.286, whole genome shotgun sequence.
ACCESSION   DACTJE010000284 DACTJE010000000
VERSION     DACTJE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.286"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            6..1551
                     /locus_tag="I9760_003012"
     rRNA            6..1551
                     /locus_tag="I9760_003012"
                     /product="16S ribosomal RNA"
     gene            582..752
                     /locus_tag="I9760_003013"
     CDS             582..752
                     /locus_tag="I9760_003013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044749.1"
                     /translation="MRRWLIKLSVKFLGLTWDGQIILVDSSMGEGSGISGVAVKCVEI
                     GRNTSGEGGYLA"
BASE COUNT          408 a          332 c          502 g          330 t
ORIGIN      
        1 gaattgaact gaagagtttg atcctggctc agattgaacg ctggcggcat gcttaacaca
       61 tgcaagtcga acggcagcat tgtctagctt gctagacaga tggcgagtgg cgaacgggtg
      121 agtaacgcgt aggaatatgc cttgaagagg gggacaactt ggggaaactc aagctaatac
      181 cgcataatgt ctgaggacga aagctgggga ccttcgggcc tggcgcttta agattagcct
      241 gcgtccgatt agctagttgg tggggtaagg gcctaccaag gcgacgatcg gtagctggtc
      301 tgagaggatg accagccaca ctggaactga gacacggtcc agactcctac gggaggcagc
      361 agtggggaat attggacaat gggggcaacc ctgatccagc aatgccgcgt gtgtgaagaa
      421 ggcctgaggg ttgtaaagca ctttcagtgg ggaggagggt tgataggtta agagctgatt
      481 aactggacgt tacccacaga agaagcaccg gctaactccg tgccagcagc cgcggtaata
      541 cggagggtgc gagcgttaat cggaattact gggcgtaaag ggtgcgtagg tggttgatta
      601 agttatctgt gaaattcctg ggcttaacct gggacggtca gataatactg gttgactcga
      661 gtatgggaga gggtagtgga atttccggtg tagcggtgaa atgcgtagag atcggaagga
      721 acaccagtgg cgaaggcggc tacctggcct aatactgaca ctgaggcacg aaagcgtggg
      781 gagcaaacag gattagatac cctggtagtc cacgctgtaa acgatgtcaa ctagctgttg
      841 gttatatgaa aataattagt ggcgcagcaa acgcgataag ttgaccgcct ggggagtacg
      901 gtcgcaagat taaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg
      961 tttaattcga tgcaacgcga agaaccttac ctacccttga catacagtga attttgcaga
     1021 gatgcattag tgccttcggg aacactgata caggtgctgc atggctgtcg tcagctcgtg
     1081 tcgtgagatg ttgggttaag tcccgtaacg agcgcaaccc ttatccttag ttgccagcat
     1141 gtgatggtgg ggactctaag gagactgccg gtgacaaacc ggaggaaggc ggggatgacg
     1201 tcaagtcatc atggccctta cgggtagggc tacacacgtg ctacaatggc cgatacagag
     1261 ggcggcgaag gggcgacctg gagcaaatcc ttaaaagtcg gtcgtagtcc ggattggagt
     1321 ctgcaactcg actccatgaa gtcggaatcg ctagtaatcg cgaatcagca tgtcgcggtg
     1381 aatacgttcc cgggccttgt acacaccgcc cgtcacacca tgggagtggg ttgcaccaga
     1441 agtagatagt ctaaccttcg gggggacgtt taccacggtg tggttcatga ctggggtgaa
     1501 gtcgtaacaa ggtagccgta ggggaacctg cggctggatc acctccttac atagaaaggc
     1561 acagaaggaa tc
//
LOCUS       DACTJE010000285         1564 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.287, whole genome shotgun sequence.
ACCESSION   DACTJE010000285 DACTJE010000000
VERSION     DACTJE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.287"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..1007)
                     /locus_tag="I9760_003014"
     CDS             complement(<1..1007)
                     /locus_tag="I9760_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT5044750.1"
                     /translation="MLSQIGNRTVSYLSNNNMDHSINIGVIIINYNSGKYLLKAARAL
                     ADQTFKPSQVIIFDNASHEEIPQEISSLNLPLTIIKHDKNLGFASGNNCAVEYLSPEI
                     NWIALLNPDAYPHKEWLEEMVHTIREYPQFAFLGSKLICEQEPHLLDGTGDCYHISGK
                     AWRRNHRKPLHQDPGSIAEIFSPCAAAALYRRDAFHAIQGFDEHYFCYYEDIDLAFRL
                     WLSGYKGAYVPKAIVEHTGSATTKRHSDFYTYHGHRNLVWTYFKNMPISLLLLSFPLH
                     LLLNIVTILLFIFRGQTSIILKSKIDAIKGLQRVLSQRKIIQKSRKINTFHLLKVLNK
                     G"
     gene            complement(964..>1564)
                     /locus_tag="I9760_003015"
     CDS             complement(964..>1564)
                     /locus_tag="I9760_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044751.1"
                     /translation="PGKDFLNQCELSVIRRNDLKQWVSELTNCIPEFKTHMQDLIRNN
                     PNTCYLLMHSNFTESKLTTIENEIALYKEILSTHAQKGNTVIIKPHPAHNPKLFLLII
                     NALKEEYKIQIIDHQYNHLPIELAESLVMGCEVLSVSYSSISLPYLYDKRVKHVLTQE
                     LVNKYFSIEKMSWFIESNNLYISMINALPNWKQDRELLI"
BASE COUNT          481 a          249 c          305 g          529 t
ORIGIN      
        1 aaacctttat tcaggacttt aagcagatgg aacgtattta tttttcgcga tttttggatt
       61 attttgcgct gacttaaaac cctttgcaaa ccttttattg catcaatttt tgattttaaa
      121 atgattgatg tttgtcctct gaaaataaat aacagaatgg taacgatatt taataaaaga
      181 tgtaatggga aacttagaag caatagggat ataggcatat ttttaaaata agtccaaacc
      241 aggttgcgat gtccatgata ggtataaaaa tcgctatgtc tttttgttgt tgcagaaccg
      301 gtatgttcaa caatggcttt aggaacataa gcgcctttat aacctgaaag ccataaacgg
      361 aaagcaaggt cgatgtcttc ataatagcaa aaataatgct catcaaatcc ctgtatggca
      421 tggaacgcat cccgtctgta tagggccgct gcagcacagg gggaaaaaat ttctgcaata
      481 ctgccaggat cttgatgaag tggtttcctg tgatttctac gccaggcttt tccgctgata
      541 tgatagcaat ctcctgtgcc atctaataaa tggggttctt gctcacaaat taattttgaa
      601 ccaaggaaag caaactgcgg gtattcacgg atggtgtgta ccatttcttc aagccattct
      661 ttatgcgggt aggcatcagg attaagtaat gcaatccaat tgatttcggg agataagtat
      721 tccactgcgc agttattacc agaagcaaat ccaagatttt tgtcatgttt aatgattgtt
      781 aagggtaaat tcagactaga aatctcttgg gggatttctt catggcttgc gttgtcaaaa
      841 atgataacct gagaaggttt aaaggtttga tcagctagtg ctcgagctgc ttttaacaga
      901 tatttcccag agttgtagtt aataatgatg actccaatat taatagagtg atccatatta
      961 ttgttagata agtagctcac ggtcctgttt ccaatttggg agagcattga tcatactgat
     1021 gtataaattg ttgctttcta taaaccaact cattttttca attgaaaaat atttatttac
     1081 aagttcttgt gtcagcacat gctttacccg tttatcataa aggtatggca gagaaataga
     1141 ggaataagat actgataaca cttcgcatcc catcacaaga ctctcagcta attcaattgg
     1201 gagatgattg tattggtggt caataatctg aattttgtat tcttctttaa gcgcattaat
     1261 tattaataaa aataatttgg gattatgtgc cggatggggt tttataataa cagtgttacc
     1321 cttttgtgca tgagttgata aaatctcttt gtagagagca atttcatttt caatggtagt
     1381 taatttagac tcagtaaaat tgctatgcat aagtaaatag catgtgtttg gattatttct
     1441 gattaagtct tgcatgtgtg tcttaaattc aggtatgcaa ttggtaagct ctgagaccca
     1501 ttgttttaaa tcatttcttc taataacaga taactcgcat tgatttaaaa aatcctttcc
     1561 aggg
//
LOCUS       DACTJE010000286         1551 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.288, whole genome shotgun sequence.
ACCESSION   DACTJE010000286 DACTJE010000000
VERSION     DACTJE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.288"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..293)
                     /locus_tag="I9760_003016"
     CDS             complement(<1..293)
                     /locus_tag="I9760_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0189 family type II secretion system
                     effector"
                     /protein_id="HAT5044752.1"
                     /translation="MRFNHFIAPLVLLPVCALSQPQNDKGDVFLTKIPTSKNAFVKSY
                     SSESAEFKLLESNARSIDYPTQIIRISGSLESQQLDCDQVHAEILEKVVAPFAP"
     gene            complement(553..>1551)
                     /locus_tag="I9760_003017"
     CDS             complement(553..>1551)
                     /locus_tag="I9760_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044753.1"
                     /translation="GISAVMILLFISTAFFTAPLWLTAITTGLFVGASTYLSGILYGV
                     VNDIFATHANLPYFLLGHQSQQTSLLRTNDKVAQGIAWGVAATFGPVIIATLIFTVAA
                     TITAFFVPIATFLLPVMMIAMPLIAVGAEFYARKKAREYLDGEENFYWIGSNDYQRRG
                     LNYMCPTNEERAAWYANSDRNLFGFTKVPLIGLGALIGLVVLSGISMFLPPVLFISPV
                     IAIAIPAAFSAVTCATLIAGGIYMQVNRNKQLDDRYRLEFERDEIVPDLYLDEDMEYI
                     RKIAKIYAQNEPTVEPKQEVINTENYSFLFDKKPKQAMPNIKQEEFEPARLSQAGA"
BASE COUNT          480 a          286 c          313 g          472 t
ORIGIN      
        1 ggagcaaaag gagccaccac tttttcaaga atttctgcgt gtacttgatc gcagtccagc
       61 tgttgtgatt cgagagaacc agagatacga ataatttgag tagggtagtc aatgcttctg
      121 gcattagatt caagtaactt gaattctgcc gattcgctgg aataagattt tacaaaagca
      181 tttttactgg ttggaatttt tgttaaaaag acatctcctt tgtcattttg gggttggctt
      241 aatgcacaaa cagggagtaa aaccagtgga gcaataaaat gattgaatct catagcaaac
      301 tcctttttta aattaaatcc tttaatttaa agaaccagtt tagcagggaa cagattagat
      361 caggttttga tcacaagtga tcgcatgaaa acaggttcta attttgagta tacaggaatg
      421 atgcaaattg gctacatcat ggatatacaa agtttgttgg aattaattcg ggcagcgctc
      481 ttttggcaat aatagagcgc tactaattga ttgagtacta aacgtgtctt tgaatccgtt
      541 catataggac tattaggctc cagcctgact cagtcgtgcc ggttcaaatt cttcctgttt
      601 tatatttggc attgcttgtt ttggtttttt atcaaaaagg aaactataat tttcagtgtt
      661 gataacttct tgtttgggct caacggttgg ttcattttga gcatatattt ttgcaatttt
      721 tctaatatat tccatgtctt cgtccagata taaatcaggt acaatttcat cacgttcaaa
      781 ctcgagacgg taacgatcat ccagttgttt attacgattg acttgcatat agataccgcc
      841 agcgattaaa gtagcgcagg taacagcaga aaaggctgct ggaatcgcta ttgctataac
      901 tggcgagata aagagcactg gtggtagaaa catgctaata ccacttagta caactaaccc
      961 aatcagcgcc cccagaccaa taagaggtac cttggtaaaa ccaaataaat ttctatcgga
     1021 gtttgcatac catgctgctc gctcttcatt agtcgggcac atgtagttta aacctcttct
     1081 ctgatagtcg ttagacccta tccagtaaaa attttcctcg ccatccagat attcacgtgc
     1141 ttttttgcga gcataaaatt ctgcgccaac tgcaattaat ggcattgcaa tcatcattac
     1201 tggtaataaa aaggtagcta ttggaacaaa aaaagccgta attgttgctg caactgtaaa
     1261 tatcagggtt gcgataatta ctggaccaaa agttgcagcg acgccccatg ctattccttg
     1321 cgctacttta tcgttagttc ttaacagact ggtttgttga gactggtggc ctaacaggaa
     1381 atagggcaag ttagcatgtg ttgcaaaaat atcatttact acgccataaa gaattccact
     1441 taaataggta gaggcaccta caaataaacc ggttgtgata gctgttaacc ataagggggc
     1501 agtaaaaaaa gcagtggaga taaacaatag aatcattacg gctgaaatac c
//
LOCUS       DACTJE010000287         1528 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.289, whole genome shotgun sequence.
ACCESSION   DACTJE010000287 DACTJE010000000
VERSION     DACTJE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.289"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            16..1413
                     /locus_tag="I9760_003018"
     CDS             16..1413
                     /locus_tag="I9760_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAT5044754.1"
                     /translation="MLIFIGISNPAISGTAPDVWDVLRSEFSLSHEVTRPEVQEQIRW
                     LISHPSYINKVSSQSEPYIYHVLGEIKKRKLPGELALLPMIESAYDPFAYSGAGAAGI
                     WQLMPRTGSDLGLKQDWWFDGRRSIGPSTDAALNYLVYLNKYFSGNWILAIAAYDSGE
                     GTISRALKSTARNGRKVQFWNLPVPRETKVYIPRLLALAEIINNPKRYKISLPNIPYL
                     PYFEEVNIGSQIDLNHAAKLAGISYKELIKLNPGYNRWTTAPYKPFKLLIPAEKVERF
                     NLNLSNIPEDKRVNWTKHQVKRGDSLDAIAKRYHTTVNLIKQLNQLTSNKVQLNQSLL
                     IPSSKNSPVAAKIPPQEKKPATKQLAPMKNVRIIHIVQNNDSYQKIQKTYGVAAKEIQ
                     NWNKLANNAPLRKGQRLIIWKRVKQPVVYIVKSGDSLSVIAKKHQTKVNTIISLNPGL
                     KKSMLLRLGQKILIG"
BASE COUNT          536 a          299 c          282 g          411 t
ORIGIN      
        1 agatttttat cattcatcct tatttttatt ggtatttcta acccggcaat ttctggcaca
       61 gctccagatg tatgggatgt attacgtagt gaatttagct taagccatga agtaactcgc
      121 ccagaagtgc aagaacaaat cagatggcta atttctcatc ctagttatat caacaaagta
      181 agtagccaat ctgaacctta catttaccac gtattaggtg agataaagaa aagaaaactg
      241 cccggagaat tggcgctact ccccatgata gaaagtgcat atgatccttt tgcctattca
      301 ggagcaggcg ctgctggtat atggcaactc atgcctcgaa ctggaagcga tcttggatta
      361 aagcaagact ggtggtttga tggaagacgt agcattggcc cttcaactga tgccgcatta
      421 aattacctcg tctatctaaa taaatatttt agtggtaact ggattttagc tattgccgct
      481 tatgactctg gagaaggaac aatttcacgg gcgctcaaat caaccgcacg taacgggcgc
      541 aaggtgcaat tctggaatct gccagttccc agagaaacca aggtatatat cccgcgttta
      601 ttagctctgg cagaaattat taacaatcca aaacggtata aaatatcact tcccaatatt
      661 ccctatcttc cttattttga agaggtcaat attggtagtc aaattgattt aaatcatgcc
      721 gctaaactcg ccggcatcag ttataaggag ttgattaaac taaatcccgg ttataatcga
      781 tggacaactg caccttataa gccatttaaa ttactaatac ctgctgaaaa agtcgaaaga
      841 tttaacttga atctatcgaa tatacccgaa gataagcgcg taaactggac taaacatcaa
      901 gtaaaaagag gtgacagctt ggatgctatt gcaaagagat atcacacaac agtcaatttg
      961 ataaaacaac tcaaccaatt aaccagcaat aaagttcaac ttaatcaatc cctactcatt
     1021 ccgagcagca aaaattcccc ggtagcagct aaaatacctc cacaagaaaa aaaacctgct
     1081 actaaacagc tggcacctat gaaaaatgtc cgtattattc atatcgtaca aaataatgat
     1141 agttatcaga aaattcaaaa aacttatggg gttgcagcta aagagataca aaactggaat
     1201 aaattggcaa ataacgcgcc attacgtaaa ggccagcggc tgatcatatg gaaaagagtc
     1261 aagcaacctg ttgtgtatat agtaaaatca ggggatagtt tgagcgttat tgccaaaaag
     1321 caccaaacaa aagtgaatac tattatcagt ttaaatccag gacttaaaaa atcaatgctc
     1381 cttcgcttgg gacaaaaaat attaattggc tagattatgt gcattatcat gatataaact
     1441 agatagatta atgcatctgt ttgaaacaga agcatgcttt tgagggaaca ccaagactac
     1501 tgcatgccgc taaaagctta aagattat
//
LOCUS       DACTJE010000288         1505 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.290, whole genome shotgun sequence.
ACCESSION   DACTJE010000288 DACTJE010000000
VERSION     DACTJE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.290"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..300
                     /locus_tag="I9760_003019"
     CDS             <1..300
                     /locus_tag="I9760_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044755.1"
                     /translation="ANYQGAPQKNYTSICHGFKKIFQQTRKNYQYHFKLKQVYRSGHL
                     AVARITWYLQIFEQGKLISETQDEGLDVFLKDAQNQWKIINFIGYPVPGNLDEKH"
     gene            385..663
                     /locus_tag="I9760_003020"
     CDS             385..663
                     /locus_tag="I9760_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044756.1"
                     /translation="MSISMITEIETLLKKAGFTYVGNGRGLGEFEKYENHLYHKNVYG
                     SIQQIQLAMDRENDTVRPVFSKNVPIELRDSIYELMKNPSEKNSLTFN"
     gene            complement(737..988)
                     /locus_tag="I9760_003021"
                     /pseudo
     CDS             complement(737..988)
                     /locus_tag="I9760_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(952..1395)
                     /locus_tag="I9760_003022"
                     /pseudo
     CDS             complement(952..1395)
                     /locus_tag="I9760_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947046.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          478 a          288 c          269 g          470 t
ORIGIN      
        1 gccaattatc agggtgctcc gcaaaaaaat tacacgtcta tttgtcacgg ctttaaaaaa
       61 atatttcagc aaacaaggaa aaattaccaa tatcatttta aattaaaaca ggtctatcgc
      121 tcaggccatc tcgcggttgc aagaattacc tggtacttgc aaatatttga gcaaggaaaa
      181 ctcatttccg agacgcaaga tgaaggatta gatgtctttc taaaagatgc acaaaatcaa
      241 tggaaaatta ttaatttcat aggataccct gtccctggta atctcgatga aaaacactaa
      301 aacaaggtgg cataataaat tgtaatatgc tccttgattg atcttgttgt attttatcaa
      361 tataatcgcc accctattta tttgatgagt attagcatga tcactgaaat tgaaacatta
      421 ctaaaaaagg cgggatttac ctatgtaggg aatggcagag gcttggggga atttgaaaaa
      481 tatgaaaatc acctctacca taaaaatgtt tatggctcaa tacaacagat ccaattggct
      541 atggacagag agaatgacac ggtgagaccc gtgttttcaa aaaatgttcc aatagagcta
      601 cgtgacagta tttatgaact tatgaaaaac ccatctgaaa aaaattcgtt aacatttaat
      661 tgacattagg gtgtgttgac aattggctcc caaagcctac gtgccgcgac ttgttcgcgg
      721 catccatgag atctaatcag ctgcagattt cttcataaag atcgcgccat tctggattta
      781 actcttcaat taaatttaac ttccactgtc tgcaccaatt tttgaaacgt ttctcacgac
      841 gagcagcttc tatgtacgtt tcatgcactt cataatagac caagatatgt acattatacc
      901 gtgctgtaaa ccctggaatc actttatttt tgtgctccca gacccactta attaaatcag
      961 atgttgagcc aacatatagg gtgccattgc tagcgttgaa gcataattcc gttttaactt
     1021 atcgtacctt gttgcaatag cacgaaactg tttaagtctg gcaaatatat tttcaactag
     1081 gtgacgatat ttatataaac cccaatcaac ttcagcatta cctgttttag agtttttctt
     1141 tctggggata attggaatgg gagatttttg cttaatttgt tctcgaattt cttcgctgtc
     1201 ataacctttg tccgcaatag tataagttgc tatcggtaaa gagctgatta agtccggtgc
     1261 agctttagca tcatgtactt caccgcaaat caactgaaat gaaattggaa aaccaaaggc
     1321 atccacagcc atatggatct ttgttgtatt gccagcgacc gatttaccaa tggcttcaat
     1381 tgttttcatt gagcataagt ctaagcattg caagctcgtt ttgtacttgg ggttagaacc
     1441 gttataatac gagtttactc ttatttatca atcaattaca cacttattcg aaggaccgat
     1501 ttcaa
//
LOCUS       DACTJE010000289         1448 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.291, whole genome shotgun sequence.
ACCESSION   DACTJE010000289 DACTJE010000000
VERSION     DACTJE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.291"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            40..1446
                     /locus_tag="I9760_003023"
     CDS             40..1446
                     /locus_tag="I9760_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISLpn6 family transposase"
                     /protein_id="HAT5044757.1"
                     /translation="MASCEWIINESKEANFGDKRLDKRYGDILSGLLSSPNASIPTTF
                     QSWNETLAAYRFFNHVNVNPESILLPHSEATLERIKAEKIVLIPQDTTEVDFTGRKSL
                     SGMGYLSNERSRGLYLHPSIAFTPERVCLGVVEMQHWIRKEIGTRNSRKGKSIEEKET
                     YCWLKGYNAANKIALAVPDTMVVSISDREGDIYEVLEKLPSEENKAYWLIRCQHDRAV
                     LNEETNQFELLLKKEVSKACVLGTIEFEIPAGTSYRNCKKRHTRKARSVRQEIRICSV
                     SLRPPRRKSKKLNVIEIQVVHCKEINTPEGEQPVEWFLITSVPIKTLDRAVEIVNWYL
                     CRWLIEMYIKILKSGCKIEELRFETYEATLNCIAFYMIVAWRVFYLTMLGRTCPDIDC
                     TTVFEDNEWQATYAMATKKKPPKKPPKLYEIILMIAKFGGHLGRGSDGFPGTKVMWIG
                     LQRMKDFTLAWETFHTLS"
BASE COUNT          500 a          234 c          313 g          401 t
ORIGIN      
        1 taatccatgt aaaattcata ttttttatat ggaacttaga tggctagttg tgaatggatt
       61 attaatgaat ccaaggaagc gaacttcggt gataagcggt tagataagcg ttatggagat
      121 atcttaagcg ggttacttag ctcaccaaat gcaagcattc caactacatt tcaaagttgg
      181 aatgaaacac tggcagccta ccggtttttt aaccatgtga atgtaaatcc ggaatcaatt
      241 cttttacccc attctgaagc gacactagaa cgtataaaag cagaaaagat agttctaatt
      301 ccccaagaca caacagaggt agattttaca ggtagaaaat cattgtcagg catgggttat
      361 ctctccaacg agagaagccg gggattatat ttacatccaa gcattgcatt tacgccagaa
      421 cgagtttgtt tgggcgtagt agaaatgcag cattggataa gaaaagagat tggtacgagg
      481 aatagtagaa agggaaagtc aattgaggaa aaggaaacct attgttggct taagggctat
      541 aatgcagcga ataaaattgc actggcagta cccgatacaa tggttgtcag tatttccgat
      601 agagagggag atatttatga ggttcttgaa aaacttccct ccgaagaaaa taaagcctat
      661 tggcttatac gttgtcagca tgatagagct gttttgaatg aagaaacaaa tcaattcgag
      721 ttacttctta aaaaagaagt tagcaaagct tgtgttcttg gcaccataga gtttgaaatt
      781 cccgcaggca cttcctatag aaattgcaaa aaaagacata ctcgaaaagc ccgtagcgtt
      841 agacaggaga ttcgtatatg tagtgtttct ttaaggcctc ctcgtcgaaa aagcaaaaaa
      901 ttgaacgtca ttgaaattca ggttgtacac tgcaaagaaa taaatacacc agaaggggag
      961 caacccgttg aatggtttct aatcacaagt gtccctataa aaacactgga tcgggcagtt
     1021 gaaattgtta attggtattt atgtaggtgg ttaatagaaa tgtacataaa aattttaaag
     1081 agtggatgca agatagagga attacgattt gaaacttacg aggctactct taattgcatc
     1141 gctttttata tgatagtggc atggcgggta ttttatttaa caatgttggg acgaacttgc
     1201 cctgatatag actgtacaac agtctttgaa gataacgaat ggcaagccac ttatgccatg
     1261 gcaacaaaga aaaaaccacc aaaaaaacca cctaaacttt atgaaattat tttaatgatc
     1321 gcaaaatttg gaggacattt aggaagaggc tctgatggct tccctggaac aaaagtcatg
     1381 tggattggtc tacaacgcat gaaagatttt actttggctt gggaaacctt tcatacattg
     1441 agttagaa
//
LOCUS       DACTJE010000290         1419 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.292, whole genome shotgun sequence.
ACCESSION   DACTJE010000290 DACTJE010000000
VERSION     DACTJE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.292"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            65..1414
                     /locus_tag="I9760_003024"
     CDS             65..1414
                     /locus_tag="I9760_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0634 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044758.1"
                     /translation="METNMTFVLKEFDALKSHFNDTVKIILQREKKDKIEDLPNPRKE
                     ELQFLTAVLNQLEAKIDELKPRSLASYVHVFYGAMLLVCKDVENNLRVMEKKENSLLF
                     TRLMDGMGISDENIPTSEQNIMFYRGLNKFLNFIYESNDSRKGLKKEHFLQVLSLKKI
                     YSLAKLSYEQEEAAENNALAKLTADGKTKANANSFHVEKPIDSSIVEQFKSWDEMKGA
                     LHQLILDELSDKNVAKISALSQARSAQLKFLQTMAEQLDKIPNQSLEPSEKMAILAGA
                     MYIVRGQIAQEYGKDPLSNDKISATVIHTGLSTILHANADCCEDKEVLIAAANKFIRH
                     MVIERPEQSNKKITKESVRENNMFSDIAGFQLISVLTLIQNMIKTCRTDAIEACVTKR
                     KEELEALKPKKEGYSIASSVTGYVGSWFKKAPSMSEEDEEDDLKDQNTAEETSKPTV"
BASE COUNT          495 a          270 c          273 g          381 t
ORIGIN      
        1 tcagcttaag tttagtttac actggccact gtaagtattt cttacattaa tcttacttgg
       61 atttttggag actaacatga cgtttgtttt aaaggaattt gatgcactaa aaagtcattt
      121 taacgacacc gttaaaatca tccttcaacg cgaaaaaaag gacaaaattg aagaccttcc
      181 caaccccaga aaagaagagc ttcaatttct gaccgctgtt ctcaatcaat tggaagcaaa
      241 aatcgatgaa ctgaaaccac gttctttggc cagctatgtc catgtatttt atggtgctat
      301 gctgcttgtc tgtaaagacg ttgaaaataa tctgcgggtg atggaaaaga aagaaaacag
      361 cttgctgttt actcgcttaa tggatggtat gggtatttct gatgaaaata taccaacatc
      421 tgagcagaac atcatgtttt acagaggatt aaacaaattc ttaaatttca tttatgaaag
      481 caatgattct cgtaaaggct taaaaaagga gcatttcctg caagtccttt cgttaaaaaa
      541 gatatactct ttagccaaat taagttatga gcaggaagag gcagctgaaa ataatgcttt
      601 ggcaaaatta actgctgatg gaaaaaccaa agccaatgcg aacagcttcc atgtggaaaa
      661 accaatcgat tcatccattg ttgagcaatt caaatcctgg gatgaaatga aaggcgctct
      721 tcatcaatta attctcgatg aactttctga taagaacgtt gctaaaattt cagctttaag
      781 tcaagcccgc tccgcacaac tgaaattttt acaaacgatg gcagaacaac tagacaagat
      841 ccctaaccaa tctcttgagc cgtctgagaa aatggccatt cttgctggag caatgtatat
      901 cgttcgtggc caaatagccc aggagtatgg aaaagatcca ttaagcaacg ataaaatcag
      961 cgctactgtg attcatacag gtttaagcac gatacttcat gccaatgctg attgctgtga
     1021 agacaaggaa gtactgattg ctgctgcgaa taaatttatt cgtcatatgg ttattgagcg
     1081 tcctgaacaa tcaaataaaa aaatcactaa ggaatccgtt cgagaaaaca acatgttttc
     1141 cgacatcgcc ggcttccaat tgatctctgt cttgacgtta atacaaaaca tgatcaaaac
     1201 atgtcgtact gatgccattg aagcttgtgt caccaagcgt aaggaagaac tcgaagcatt
     1261 aaaacccaaa aaagagggtt attccattgc gagttcagtc actggatatg taggcagctg
     1321 gtttaaaaaa gcaccaagca tgtctgaaga agacgaagaa gatgacttaa aagatcaaaa
     1381 cacagcagaa gagaccagca aaccgaccgt ataattaat
//
LOCUS       DACTJE010000291         1389 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.293, whole genome shotgun sequence.
ACCESSION   DACTJE010000291 DACTJE010000000
VERSION     DACTJE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.293"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            32..1219
                     /locus_tag="I9760_003025"
     CDS             32..1219
                     /locus_tag="I9760_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAT5044759.1"
                     /translation="MFKSVQQRTIQNGQVIPFYHSTNELAHIQNILQSQTPLVSLKEI
                     NKFRQLIARASVGEYFLIQSGDCAENFYECDKDTTHAKLDFIRHLATFFHQKTGCSVI
                     QVGRIARQYAKPRSNQYEICQNKRIYSYFGGMINQSQETQRRPNPWRILLSYNCSASI
                     YRKINQWNRGINPEQKIYTSQEAFLFNYEQSLTRWDERTGLYYNLSTHFPWLGKRTVS
                     SLQHINHLKKIANPIAVKIGPDTPIKNLVKVINELDPSYIPGRITLIPELGAQNVYHI
                     LPQLIDAVKRTDRTVLWSCDPMHGNTETLSSGIKIRKLSNIVEEIKNSFFIHRKMGSQ
                     LNAIHLEATHQNVTECMDYKSSHCESISDLKKNYKNLLDPRLNYAQTLHVIQCVAKYY
                     ANS"
BASE COUNT          518 a          239 c          237 g          395 t
ORIGIN      
        1 gtaaatcttt aagttacaag gaaatgtaaa tatgtttaaa tcagtccaac aacgtacaat
       61 ccagaatggt caagttattc cattctatca ttccactaat gaactggcac atatccaaaa
      121 tattctacag tctcaaactc cattggtatc tttgaaggag attaataaat tccgtcaatt
      181 gatagcaaga gcatcggtag gagagtattt tttaatacaa tcgggtgact gtgccgaaaa
      241 tttttatgag tgtgacaaag acacaaccca tgcaaaatta gattttattc gtcacttagc
      301 cacatttttt catcagaaaa ctggctgtag cgtgatccag gttggtcgga ttgcaaggca
      361 atatgcgaag cctagaagca atcagtatga aatttgccag aataaaagaa tctattcata
      421 ttttggtggc atgattaatc agtctcaaga aactcaacga cgtcccaacc cttggaggat
      481 actgttgagt tataattgtt cagcatctat ttatcgcaag attaatcaat ggaatcgagg
      541 aataaaccca gaacaaaaaa tatatacgag ccaagaagct tttttattta attacgaaca
      601 atcattaaca cggtgggatg aacgaacagg attatattac aacctttcaa cacatttccc
      661 atggcttggt aaaaggacag taagcagctt acagcatatt aaccatttaa aaaaaattgc
      721 aaatcctatt gccgtgaaaa taggcccaga tacccctatc aagaaccttg taaaggtcat
      781 caatgaatta gatccaagtt atattcctgg acgtatcacc cttattcctg aattaggagc
      841 gcaaaatgtc tatcacattt taccgcagtt aattgatgcg gtgaaaagaa cagatcgcac
      901 tgttttatgg agttgtgatc ccatgcatgg aaatactgaa actctaagtt cgggaataaa
      961 gattagaaaa ctaagtaaca ttgttgagga aatcaaaaat tcatttttta ttcatagaaa
     1021 gatgggtagc caacttaatg ctatccatct tgaagcaaca catcagaatg tcacagagtg
     1081 catggattat aaatcatctc attgtgaatc aataagcgac cttaagaaaa actacaaaaa
     1141 tctattggac cctcgattaa attatgcaca aacgctgcat gtcattcagt gtgttgcaaa
     1201 atattacgca aattcttaat aaaaggtctg gacatggacg aactaaaaaa aagagcttat
     1261 ttagctttaa ccaatatatc cagaaaagta attttaacac tgttaattga attaaggagt
     1321 aattatgagc agtttgaaaa atgaatatta taaaagaacc caaaaagaaa aagattttca
     1381 aaattacaa
//
LOCUS       DACTJE010000292         1365 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.294, whole genome shotgun sequence.
ACCESSION   DACTJE010000292 DACTJE010000000
VERSION     DACTJE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.294"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..202
                     /locus_tag="I9760_003026"
     CDS             <1..202
                     /locus_tag="I9760_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5044760.1"
                     /translation="LKNNQSNQTLIKAIIAMAKKFNLSLIAEGIENQYQLEFLKKLGC
                     HYGQGYYFSHPKPTEKCMQFLQ"
     gene            complement(204..773)
                     /locus_tag="I9760_003027"
     CDS             complement(204..773)
                     /locus_tag="I9760_003027"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HAT5044761.1"
                     /translation="MTIPDKIKAVYEKSTCLYTSNEVEAALDRMAIKIHETLQDKNPV
                     IICVMVGGLVPLGNLLHRLDFPLEVDYVHATRYRGDLTGGDILWKVRPSSNLAGRTVL
                     VVDDILDGGITLAAIINEIKAMGAAEVYSAVLVDKYRKRVPNGLQKADFVGLQVEDHY
                     IFGYGMDYHEYLRNAPGIFIVHPDHEASK"
     gene            complement(816..1142)
                     /locus_tag="I9760_003028"
     CDS             complement(816..1142)
                     /locus_tag="I9760_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HAT5044762.1"
                     /translation="MTKKSLFKISFANQDEIYEIYARSIKESDMFGFLEVEELVFGEQ
                     TALVVDPSEEKLKMEFCDVKRIFIPVHSIFRIDEVSKQGTSKVKDNLSHGNKVRPFPG
                     SGKIKD"
BASE COUNT          442 a          268 c          209 g          446 t
ORIGIN      
        1 tctaaaaaat aatcaatcca atcaaactct aataaaagct ataattgcga tggcaaaaaa
       61 atttaacttg agtttgattg cagaaggaat cgaaaaccaa tatcaactcg aatttttaaa
      121 aaaactgggc tgtcattatg gacaaggata ttatttttct catccaaagc caaccgaaaa
      181 atgcatgcaa tttttgcaat aaattattta cttgcctcat gatccggatg aacgataaaa
      241 atccctggcg cattacgtaa gtactcgtga taatccatac catagccaaa aatataatga
      301 tcctctactt gtaaaccgac aaaatcagcc ttttgcaaac cattggggac acgtttacga
      361 tatttatcaa ctaatacagc actataaact tccgcagccc ccatggcttt gatttcatta
      421 attatggcag ccaaggttat accaccatca agaatgtcat ctacgaccag gactgttcgt
      481 cctgcaagat tgcttgatgg tctcactttc caaagaatgt cccctcccgt taaatcacct
      541 cgataacgcg tagcatgaac ataatccacc tctagaggaa aatccaaacg atgcaataga
      601 ttacctaatg gaactagacc tcctaccatc acgcaaataa ttacagggtt tttatcctgt
      661 agtgtttcat gaatttttat tgccattctg tccagtgctg cttcaacttc gttagatgtg
      721 tataaacagg ttgatttttc atatacagct ttaattttat caggaatggt catttattta
      781 tttccttaga agttgtttat acaggctaaa cgaagttaat cttttatctt tcctgatcca
      841 gggaaaggcc ttactttatt gccatggctc aagttatctt tgacttttga tgtcccttgt
      901 tttgaaactt catcaatgcg aaatatggaa tgtacaggaa taaaaatgcg cttaacatca
      961 caaaattcca ttttaagttt ttcttcagac ggatcaacta ccagtgccgt ttgctctcca
     1021 aaaaccagct cctcaacctc cagaaaacca aacatatcgc tttcttttat tgaacgagca
     1081 tagatttcat aaatttcgtc ctgattagca aaactgattt tgaatagcga tttttttgtc
     1141 atgtatctaa ccttatctaa aagcaggata aagtatacaa aaagtaaggg tgtatcttca
     1201 aatatttaaa gcagtttagt cgtctagagc ttattattag catcaaattt attcatttag
     1261 acttaatggt gacaataatt taatctcttt cgtatgtgga atattaatga aatataagtt
     1321 tcatctaatt tgttgctggc tgtaagggcg agtcatactc ttttt
//
LOCUS       DACTJE010000293         1351 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.295, whole genome shotgun sequence.
ACCESSION   DACTJE010000293 DACTJE010000000
VERSION     DACTJE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.295"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..629
                     /locus_tag="I9760_003029"
     CDS             <1..629
                     /locus_tag="I9760_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT5044763.1"
                     /translation="VINIAINAGFESHEAFSRAFKKACGFSPSQFRQGFGRSYWEQPP
                     YCLPGQSRTDMKVDIKSIDKIRLAVIEHKGDPKLLGESINKLVSWAKSQSINLKPRAG
                     EAFALAYDDPKTTPPSDFRIDLGIKVPENLKLDGVIEKFLPSGRYAVTVHKGSRNNIG
                     DVVYYLYRDWLPNTSEQLGDLPCIFCYYNFDHEVAETELLTECWLLLK"
     gene            complement(901..>1351)
                     /locus_tag="I9760_003030"
     CDS             complement(901..>1351)
                     /locus_tag="I9760_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044764.1"
                     /translation="FAKHRFVISALYGSKHSPVFNEALTVMHETYQVKSLQDAIRENP
                     EKSDYLRSYYSASLMLSRFVKRLYTNEQDYQKMIEDCQNEFGNPTAFQIIQDGSWGEG
                     LNAVLMESKHLAATTIQRFFRVAQALNQVNSKQPTTYTKSSQKPRIF"
BASE COUNT          396 a          234 c          277 g          444 t
ORIGIN      
        1 ccgtcattaa cattgcaatc aatgcaggtt ttgagtctca tgaagccttt tccagggcct
       61 ttaagaaagc ttgtgggttt agtccaagtc agtttaggca aggttttggg aggtcatatt
      121 gggagcagcc accgtattgt ttgcccggac agagtagaac tgatatgaaa gtagatatta
      181 aaagtattga taagatacga ttagctgtaa ttgagcacaa aggcgatcct aaattgctgg
      241 gtgaaagcat taacaagctg gtatcctggg caaaatctca atcgattaat cttaaaccac
      301 gcgccggaga agcgtttgcc cttgcttatg atgaccctaa aacaacacca ccatctgatt
      361 ttcgtattga tcttgggatc aaggtccccg aaaatttaaa attggatggt gtgattgaaa
      421 agtttctgcc atcaggtcgt tatgcagtga cggtacacaa aggttcgcgt aataatattg
      481 gtgatgtggt ttattatctt tatcgagatt ggttacccaa tacctcagaa caattaggag
      541 atttaccctg tatcttctgt tattacaatt ttgatcatga agtagctgaa acggaacttt
      601 taacggaatg ttggcttttg ttaaaataat ccgctttaaa cagcagtcag aatagctgat
      661 aacaatagat cgggcataac cggcatcttt cattttattg ctgtgttaca aacgtttctg
      721 aggcaatttt tactatttat aacttcgctc ctcaaaacgt ttgtgccttg cctaaaacaa
      781 aataatcagc ttatgcaaga ggtcttttat tattatggta ctttataaaa gtacataaat
      841 aaaaataaaa cgcgtgctaa ttggttttat tgcgtcttta aatagttagt caatttgtct
      901 tcagaagatc cttggttttt gagaggattt agtgtaggtc gttggctgtt tggaattcac
      961 ttgattcaga gcttgtgcta cccgaaaaaa tctttgtatg gtagttgctg ccaggtgttt
     1021 gctttccatc aaaacagcat ttagtccctc tccccatgaa ccatcttgta taatttgaaa
     1081 tgcagtaggg ttcccaaact cattttggca gtcttctatc attttttgat aatcctgttc
     1141 atttgtataa agtcttttaa caaaacggga taacataaga gatgcggaat aataggaacg
     1201 aaggtagtct gatttttccg gattttctct aatggcatct tgtaatgatt ttacttgata
     1261 agtttcatgc atgacggtca atgcttcatt gaatactgga gagtgcttgc tgccataaag
     1321 cgcagaaata acaaaccgat gcttggcaaa t
//
LOCUS       DACTJE010000294         1347 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.296, whole genome shotgun sequence.
ACCESSION   DACTJE010000294 DACTJE010000000
VERSION     DACTJE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.296"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(71..>1347)
                     /locus_tag="I9760_003031"
     CDS             complement(71..>1347)
                     /locus_tag="I9760_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lpg1449 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044765.1"
                     /translation="EIFNRRFMSTPLASLEQTLNHSDATKSFYRLQFSRQELEKAKEF
                     VFQLAIPNEFSKQEDFDFFYGEHGNKLEETTKLAILMLKRMVPKEFHHKIRIGINMCC
                     PAASVENIGSGFGLRNKPDFIERIVKAIKDADSNLKVEFKTLMLSHEDGYGDGDQISG
                     NTKNISEIQNECLTRIVYKTKANRLVYQGKPRNIETYNVDAVSCIIREVNTPKEFQFG
                     YSGMVATITNHDINQLGYKVVGEPHYSVQSILEKIKKQLGEVPIEFEVMLGRVLLGSA
                     WVLQGRYASDAEILLHTLFQAAIEQEWARGMGRYGIDIYQIQVLYNIRHITNDNYRNS
                     LINKIFNAQSTEEIIDKIFELYFSIKKKGECNILQLQRLEKAVFAAIPNSKALQQYKL
                     ITESNSKSRAGFQFFSNKDKKQTLEEKSVFTP"
BASE COUNT          408 a          268 c          188 g          483 t
ORIGIN      
        1 ctttttaaac catgaataac tatctaatgt aactatcaat atcaaccata ctttaattta
       61 caatactatc ttaaggagtg aagacagatt tttcctctag tgtttgcttc ttatctttat
      121 ttgagaaaaa ttgaaaacct gccctcgatt ttgagtttga ctctgtaatt aatttatatt
      181 gttgaagagc tttgctatta ggtattgcag caaataccgc tttttcaagt ctttgaagct
      241 gtaaaatatt gcattcccct ttcttcttaa tgctgaagta tagttcaaat atcttatcaa
      301 ttatttcctc tgtagattga gcattaaata ttttattaat tagagaattc ctataattat
      361 cattagttat atgtcgaata ttatatagaa cttgaatttg atatatatca ataccatatc
      421 gccccatccc cctggcccat tcttgctcaa tcgctgcctg aaacaacgta tgtagaagaa
      481 tttctgcatc actggcatat cttccttgta aaacccatgc tgagcctagc aaaactcgac
      541 ctagcatcac ttcaaattca attggaactt cccctaattg ttttttaatt ttttcaagaa
      601 tactttgtac actgtaatgt ggctcaccga caaccttata acctaattgg tttatatcat
      661 gatttgtaat agtagctacc attccgctat acccaaactg gaattccttt ggtgtattga
      721 cctctctaat aatacacgac actgcatcta cgttataagt ttcaatattg cgtggctttc
      781 cttgatacac caatctatta gccttcgttt tataaactat tcgtgtgaga cattcattct
      841 gaatttccga gatatttttt gtattaccag atatttgatc accatcacca tagccgtctt
      901 catgtgataa cattaaagtt ttaaattcaa ctttcaagtt tgaatcagca tcctttatcg
      961 ctttaacaat gcgctctata aaatcaggct tattacgcaa accaaagcct gaaccaatgt
     1021 tttccactga tgcagcaggg cagcacatat taataccaat acgaatttta tgatgaaact
     1081 ctttgggcac cattcttttt aacatgagaa tagcaagctt tgttgtttct tctaatttat
     1141 tgccgtgctc accataaaaa aagtcaaaat cttcttgctt ggaaaattca ttggggatag
     1201 ccaactgaaa aacaaactct ttagcctttt ctaattcctg acgactaaat tgcaatcgat
     1261 aaaagctttt tgttgcatct gaatgattaa gtgtttgctc caaggacgct aacggtgtgc
     1321 tcataaaccg tctattgaaa atttcag
//
LOCUS       DACTJE010000295         1345 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.297, whole genome shotgun sequence.
ACCESSION   DACTJE010000295 DACTJE010000000
VERSION     DACTJE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.297"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            290..1135
                     /gene="hslO"
                     /locus_tag="I9760_003032"
     CDS             290..1135
                     /gene="hslO"
                     /locus_tag="I9760_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAT5044766.1"
                     /translation="MKEADTLQRFIFEHANIRGEIVHIEKTFQNIMNQRDYPPMVKNL
                     LGEALVSCLLLASSIKFEGNLSLQFQGDERLSLLLVQCDHNLNIRGFAKCAEGLEIAD
                     YATAFLQGRMVITLSQDNQTQAYQSLIPIQSTSMSENLMTYFAQSEQIATRVWLAVNE
                     DMAAGMLLQLMPGQDTIQREQFWEYAVQLGQTVTEGELLTIDNQTLLYRLYHETELRI
                     FESRSTRFQCRCNQEKMKQVITVLGEEEATDLIKEKGKIEITCDFCNQKYTFDSIDVT
                     LLFRK"
     gene            complement(1149..>1345)
                     /locus_tag="I9760_003033"
     CDS             complement(1149..>1345)
                     /locus_tag="I9760_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044767.1"
                     /translation="FSDMGQAAYLTVGELEENQTANLYLQLSEQFITLQQVLQAMRGE
                     YIQFSQSSCGVLAEPDLTMQ"
BASE COUNT          458 a          249 c          238 g          400 t
ORIGIN      
        1 ctatttcttt aatttcttct ataccaaaca ataatttgtc tattgtttta aataggggaa
       61 gaagtaaact gttatttttc ataataactc cttacaattt taaaaagggg ttttttaaga
      121 atagtccatg aaataaattt ctccaggaaa attaaaaaat aattaagata cgctggttat
      181 ggaaactact attcctcttg taagattttg ttcaaaataa ctgaactcag aaaaggcctg
      241 agataaacat tttatgttag aataggtcta tttgtgttat gagtttgaaa tgaaagaagc
      301 agatacctta cagcgtttta tttttgagca tgcgaatatc cgtggcgaaa tcgttcatat
      361 tgaaaaaaca tttcaaaaca ttatgaacca acgtgattac ccacccatgg taaaaaatct
      421 tttaggtgaa gcgttagtct catgtttact cctggcaagc agcattaaat ttgaaggaaa
      481 tttaagtctg caatttcaag gtgacgaaag attatccctg ttactcgttc aatgtgatca
      541 taatttaaat ataagaggct ttgcaaaatg tgcagagggt cttgaaattg ctgattacgc
      601 gaccgctttt cttcaaggcc gaatggtcat tacgctcagt caagacaacc aaactcaagc
      661 ctatcaaagc ctgataccta ttcaatccac gtccatgagc gaaaatctga tgacttattt
      721 tgcccaatca gagcaaattg ctactcgtgt ttggttagct gtcaatgaag acatggctgc
      781 aggtatgcta ttacaactta tgccaggtca agatacaatt caaagagaac aattttggga
      841 gtatgccgtt cagctgggac aaacagtaac tgaaggtgaa ttgctgacca tagacaatca
      901 aacactactc tatcgtcttt atcatgaaac ggaattaaga atattcgaaa gccgatctac
      961 ccgtttccaa tgccgttgta atcaggaaaa aatgaaacag gtcattactg tactgggaga
     1021 agaagaggca acagatttaa tcaaagaaaa aggaaaaata gaaatcactt gcgatttttg
     1081 caatcaaaaa tacacttttg attcaatcga cgtgactcta ctatttcgca aataaaatga
     1141 gcctctgcct actgcatagt caaatcgggt tctgccagaa cgccacaact tgactgacta
     1201 aactggatat actctcctcg catggcttgt aatacttgct gcaaagtaat aaattgctca
     1261 cttaattgca aatacaaatt ggctgtctga ttttcctcaa gctctccgac agtcaaataa
     1321 gccgcttgcc ccatgtcaga aaaat
//
LOCUS       DACTJE010000296         1344 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.298, whole genome shotgun sequence.
ACCESSION   DACTJE010000296 DACTJE010000000
VERSION     DACTJE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.298"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            250..1185
                     /locus_tag="I9760_003034"
     CDS             250..1185
                     /locus_tag="I9760_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT5044768.1"
                     /translation="MNEIQEFLRQKLNSPLPISRPQLAKLPMGTMEFVTYGNGKPVLI
                     IHGGGGGYDQAVLLFKRYIPDGYQMICPSRPGYLGTPIDTGQTAEEQADALAGLLDYL
                     NITSAVIVAISAGGLSLYPFAIRHPKKTKAIIAIDAIAGEYLMPEQSNKLAQALFMTD
                     FGLWVAKKSMIYFPKSFIKNLIKLEGYLDPVRVDDRVKEILNCRERLEIIAELMNSMA
                     DYKARKTGTVNDMAEGVKTSWYDFSKINCPALIIHGTHDADVKFYNGVFAYERLASKE
                     KERFWIEYGTHLGFFFAHQAREVQQKFQEFIIKHG"
BASE COUNT          426 a          238 c          281 g          399 t
ORIGIN      
        1 atctcattta tcattcgcca agagtgtaag ggtttaattt tgctttgttc aggcccaata
       61 taattaatta atgcttgcaa gcatctcatt tctgataggt cttaaatgga aacaaaatag
      121 aacatattta tcaaaaagaa attagatggc aaaaaggtct tttcataaat aattttcgat
      181 attttcagta gacaagatca tctaaaaagc caagtattaa tttgctttca ctttggatag
      241 ggagttgcca tgaatgaaat tcaagaattc ttgcgtcaaa aactgaattc gccgttacct
      301 atttcacgac ctcaattggc aaagctacca atgggtacta tggagtttgt tacttatggt
      361 aatggaaaac ccgtactgat tattcatggt ggagggggtg gttatgatca ggcagtgctt
      421 ttattcaaac gctatatccc tgatggttac caaatgattt gtccttctcg tcccggttat
      481 ttgggaacac ccattgatac tggacaaaca gcagaagaac aggcagatgc cctggccggc
      541 ttactggatt atttgaatat taccagcgca gttattgtag ccatatcagc tggagggctt
      601 tctctgtatc cttttgccat tcgccacccg aaaaaaacta aagcaatcat tgcaatagat
      661 gctatagctg gagaatattt aatgcctgaa caatccaaca aattggctca agcccttttt
      721 atgactgatt ttggtttatg ggtcgctaaa aaaagcatga tttatttccc caagtcattt
      781 atcaagaatt taattaaact ggaaggttac ctggatccag tacgtgtaga tgaccgggtg
      841 aaagagatct taaattgtcg ggagcgcctg gaaatcattg ctgaactaat gaatagcatg
      901 gcggactata aggcccgtaa aacagggacc gtgaatgaca tggcagaggg tgtaaaaacc
      961 agctggtatg atttttctaa aattaactgc cctgcactaa taattcatgg gacacatgat
     1021 gctgatgtaa aattttacaa tggtgtattt gcttatgagc gattagcatc gaaagaaaaa
     1081 gagcgcttct ggattgaata cggcactcat ttggggtttt tctttgctca tcaggcgcgt
     1141 gaggtgcaac aaaaattcca ggaatttata ataaagcatg gttaaacagt ttcttgttga
     1201 aaagttcatg gcggcagaat aaacaacttc taaatctatc tactaaataa cgtgagttat
     1261 agataaagag gatataaaaa ccagtcgaac cccaggttac gtttgggctg aggagataca
     1321 taagcatcgt ctcgaagcct tgac
//
LOCUS       DACTJE010000297         1290 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.299, whole genome shotgun sequence.
ACCESSION   DACTJE010000297 DACTJE010000000
VERSION     DACTJE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.299"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..987
                     /locus_tag="I9760_003035"
     CDS             <1..987
                     /locus_tag="I9760_003035"
                     /inference="COORDINATES: protein motif:HMM:NF017011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HAT5044769.1"
                     /translation="FMEDFSRYFYDKHKFIADIRNILKNEESKSSEVTRTEKKIPFSS
                     FSKKLQHILTNFKLDKEHEIDIDNTDLKLGDYLTNYFDHFEDDLDKLESLSSALLKFV
                     KYAQYEKRLADLSQKTVDLTVPYVYFPLSFQPESTTECLGGHFADILTAVEKIRCLIP
                     PDWKIYLKDHVIQYELARDKIFFERLKSIPNLILVDRFHSSFELIRNSQFVATLNGTV
                     GWEAIHMGKKAVTFGGIYYQGLPGVFLYSQSLKLDDILNTSIDKEILEDAINTLIGRM
                     EIGVVNADYSAIVENFNEETNANYILDFIQRFFFEQHSSTLSNLREYTYEAS"
     gene            974..>1290
                     /locus_tag="I9760_003036"
     CDS             974..>1290
                     /locus_tag="I9760_003036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044770.1"
                     /translation="MKLLKKISFLESANFSPWPNRLIGLSPWQKSERTTKEVLDEYNN
                     VWYKNALREWENYSNTNQSPSAARFFRVLDNIIYNQMLQNFEIYGISSDEHLISINRE
                     LYTA"
BASE COUNT          459 a          208 c          201 g          422 t
ORIGIN      
        1 tttatggagg atttttcacg gtatttttat gacaagcaca aatttattgc tgatatcaga
       61 aacattctca aaaatgaaga aagtaagtca tcagaagtaa cgagaactga aaaaaagata
      121 cctttttctt catttagcaa aaaattgcaa catatattaa ctaatttcaa acttgataag
      181 gaacatgaaa ttgatatcga taatacggat ttaaaattag gagattattt aaccaattac
      241 tttgatcatt ttgaggatga tttggataaa ttggagtctc tatcttcggc attactaaaa
      301 ttcgtaaaat atgctcaata tgaaaaaaga cttgctgatt tgtcacaaaa aactgtcgac
      361 ctaacagtac catacgttta ttttccatta agctttcaac cagaatcaac gactgaatgt
      421 cttggagggc atttcgcaga tattcttact gcagtcgaaa aaatccgttg cttaattccc
      481 cctgattgga aaatctattt aaaagatcat gtaatacaat atgagttagc aagagataaa
      541 atattctttg aacgtttaaa atctataccc aacttaatac ttgtagatcg atttcattct
      601 tcatttgagc taattagaaa ctctcaattt gtagctacct tgaacggcac agtcggttgg
      661 gaagccattc atatgggtaa aaaagcagtt acctttggtg gtatctatta tcaaggtctt
      721 cctggagtat tcttatattc tcaatcacta aaactagatg acattctaaa tacatcgatt
      781 gataaagaaa ttttagaaga cgctattaat actcttattg gaagaatgga aataggtgtt
      841 gtaaatgcag actactctgc aattgttgaa aattttaatg aagaaacaaa tgcaaactat
      901 atcctggatt tcatacagcg ctttttcttt gaacaacact catctaccct aagtaattta
      961 agagagtaca cctatgaagc ttcttaaaaa aatttctttt cttgagtctg ctaacttttc
     1021 accttggcca aatagactta taggattatc tccttggcaa aaaagtgaaa gaacaactaa
     1081 agaagtacta gatgagtata ataatgtttg gtataaaaat gcccttcgtg aatgggaaaa
     1141 ctattcaaac actaatcaat ctccttccgc agcccgtttt ttcagagttt tggataacat
     1201 tatctataat caaatgctac aaaactttga aatttatggc atctctagtg atgagcattt
     1261 aattagtata aatcgtgagt tatacactgc
//
LOCUS       DACTJE010000298         1235 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.300, whole genome shotgun sequence.
ACCESSION   DACTJE010000298 DACTJE010000000
VERSION     DACTJE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.300"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..>1235
                     /gene="legC1"
                     /locus_tag="I9760_003037"
     CDS             <1..>1235
                     /gene="legC1"
                     /locus_tag="I9760_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC1"
                     /protein_id="HAT5044771.1"
                     /translation="TRLYAQILGVEQNRGEEKDLAKLTRDLINLQNGVVTSLLKTMPL
                     ITDDTSDDEHANKYAQKNFYSPAGMSALFAPLYAAAKVFSYNNEMPISYGSDPYAYFE
                     LLLSWNKTLDESQFVRSEVVKEKLENRAFKMLLENFANESNKTGLVKMGRSEVTICFK
                     NIYTEIYSNQIERKKLDVDSIINSNLTAIKNALEINTENDRGAIQFNEEKLRLILKQG
                     INIIQQSFPKYMEHVNSLENNPTVYFADNNPCVNKDHLEVRTAIEVLKDLCTKDLCPK
                     VFVLDTTSATEAQIEDFLNVFNQQDKIPILVTAASMVKHSEFGLDLWQGGINKAYLSE
                     KSEKNENSQNEFARFITELKNVTKGTEPGFTRFARRHVREAMNNINEAIKDSQDNSEP
                     QDATCKDDVQNIPTNPISE"
BASE COUNT          464 a          190 c          231 g          350 t
ORIGIN      
        1 actcgtcttt acgcgcaaat acttggtgtt gaacagaatc gtggagaaga aaaggatcta
       61 gctaagttaa cgagggactt gattaatctt caaaatgggg ttgtcacttc attattgaaa
      121 actatgccct tgattacgga tgataccagt gatgatgagc atgccaataa atatgcacaa
      181 aaaaattttt actcaccagc aggtatgagc gctctatttg cgccgcttta tgcagcagca
      241 aaagtgttta gttataataa tgagatgcca atttcttatg gcagtgaccc ttatgcatac
      301 tttgagttat tattatcttg gaataaaacc ttagacgagt cacaatttgt gcgttctgaa
      361 gttgttaaag aaaaacttga aaatcgcgct tttaagatgc tattagagaa ttttgcaaat
      421 gagtcaaata aaactggctt ggtcaaaatg ggaagatctg aagtaacgat ctgttttaaa
      481 aatatttata ctgaaattta tagcaatcaa attgaaagaa aaaaacttga tgtagattca
      541 attattaatt caaatttaac agcaattaaa aatgctttgg aaataaatac tgaaaacgat
      601 aggggggcaa tccaatttaa tgaggaaaag cttaggttaa tattaaaaca aggaataaat
      661 attatccaac aatcttttcc aaagtatatg gagcatgtaa atagcctgga aaacaatcct
      721 acagtgtatt ttgctgataa caatccttgt gttaacaaag atcatctgga ggtaagaact
      781 gccatagaag ttttaaaaga tctttgcact aaagatcttt gcccaaaagt gttcgtcctg
      841 gacacaactt ctgcaactga agcccaaata gaagattttt taaatgtgtt taatcaacaa
      901 gataaaattc caattcttgt gactgctgca agtatggtta aacacagtga atttggatta
      961 gatttatggc agggaggaat caacaaagcc tatttgtcag aaaaatcgga gaaaaatgaa
     1021 aattcgcaaa atgagtttgc acgctttata acggaactca aaaatgttac caaaggaacc
     1081 gaaccaggct ttacacgatt cgcaagacga catgttcgag aagcaatgaa taatataaat
     1141 gaggcgataa aagattcaca agataatagt gaacctcaag atgcaacatg taaagatgat
     1201 gtgcaaaaca taccaactaa ccctatttct gaaga
//
LOCUS       DACTJE010000299         1235 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.301, whole genome shotgun sequence.
ACCESSION   DACTJE010000299 DACTJE010000000
VERSION     DACTJE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.301"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            16..555
                     /locus_tag="I9760_003038"
     CDS             16..555
                     /locus_tag="I9760_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT5044772.1"
                     /translation="MNAYTKKSLCSQIAIVPQHLTLFHRSIYDNITYGCNNFDKSDVI
                     LAAKKAHCHEFISELPQHYDTIIGEQGVKLSGGQRQRIAFARAILKNAPILLLDEATS
                     ALDSSTEQAIQDGLQNLLADKTALVIAHRLSTLKAMDRILVLEDGKIIENGTHTELLE
                     KKGAYYNYWTHQSEGFIRA"
     gene            846..>1235
                     /locus_tag="I9760_003039"
     CDS             846..>1235
                     /locus_tag="I9760_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HAT5044773.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGHGDITSGSIYNGVPTIDTEAL
                     NRQGFMPVLTGVGPRRDSGHWIMLIKGPGNQYFLFDPLGKTSGEGYKNTLLAQLPIAS
                     TLSVIPNEPGLNKGLCGYWVASVGLKAR"
BASE COUNT          419 a          245 c          220 g          351 t
ORIGIN      
        1 atagatgact tgccaataaa tgcctacact aaaaaatcac tttgctctca gattgcaata
       61 gtgccacagc atttaacact ttttcatcgt agcatttatg ataatatcac ctacggttgc
      121 aataattttg ataaatcaga tgtgatttta gctgcaaaga aagcacactg ccatgagttt
      181 atctctgaac tcccacaaca ttatgacact attattggcg agcaaggcgt aaaattatca
      241 ggtggacaaa gacagcgtat tgcatttgcc cgagctattc tcaaaaatgc acctatcttg
      301 ttacttgatg aagcgacatc agccttagac tccagtactg aacaggcaat tcaagatggc
      361 ttacaaaatt tgttagcaga taaaactgct ttagtgatcg cgcaccgtct atcaacgctc
      421 aaagcaatgg atagaattct agttttagaa gatggcaaga tcatcgagaa tggaacacat
      481 accgaactat tagaaaaaaa aggagcctat tataactact ggacacacca atcggaaggt
      541 ttcatacggg cataaagtaa cctgatatca gcctgtaaac tgttaatata atcctaagaa
      601 aaacttataa ctattaaaac attaaagtct tatatcattg tatcaactcc tcttggctta
      661 tttttaaaat agaaagctct tactgtctat caaattcccc aatcacaaaa aatcaaaaga
      721 ctaattttaa acacttaata tttccttaaa actattatcc tataattaag tatacaaagt
      781 gtgaatattc gtatccatta gaatcagtat ttgcatgtac aaacatcagt ttgggagaaa
      841 gtaaaatgcc taaatacgta gaaggggtag aattaactca agaaggtatg catgctattt
      901 ttgctcgtat ggggcatggc gatatcacca gcggtagcat ttataatgga gtaccgacta
      961 ttgacacaga ggccctgaac agacaaggct tcatgcctgt tttaacagga gtaggcccca
     1021 gaagagattc tggtcactgg atcatgttaa tcaaagggcc tggtaaccaa tattttctct
     1081 ttgacccact gggtaaaaca tcaggcgaag gctataaaaa tactttatta gcccaattac
     1141 ccatagcctc tactctttct gttatcccca atgagcccgg cctaaacaag gggctatgtg
     1201 gctattgggt agcctctgtt ggattaaagg cacgt
//
LOCUS       DACTJE010000300         1233 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.302, whole genome shotgun sequence.
ACCESSION   DACTJE010000300 DACTJE010000000
VERSION     DACTJE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.302"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..813)
                     /locus_tag="I9760_003040"
     CDS             complement(<1..813)
                     /locus_tag="I9760_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214018.1"
                     /note="ligates
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate to
                     UDP-N-acetylmuramic acid for reincorporation into
                     peptidoglycan; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAT5044774.1"
                     /translation="MMTHLHILGVSGTFMSALALLARDAGYRVTGSDANCYPPISDLL
                     AAKGISWTEGYDDSTLALQSDMVIVGNAIKRGMPVLEAVMESGKPYMSGPQWLAENIL
                     SKYQVIAVAGTHGKTTTTSMIAWILDQAGMNPGFLIGGVSSDFNTSACLGKGRWFVIE
                     ADEYDSAFFDKRPKFMHYRPNIAVLNNLEFDHADIYPDLAAIQQQFHYLLRTIPKSGV
                     AIKPRDDKALNEVLSRGQFSRIETLALSGEAEWVAELIDDSGSQFKILHNGGV"
     gene            complement(815..>1233)
                     /locus_tag="I9760_003041"
     CDS             complement(815..>1233)
                     /locus_tag="I9760_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044775.1"
                     /translation="QWNIAELDFSRDWLLSLMMATSLILSFIFYTYVILKFQNLASRL
                     VQTSTCLLSAYIIIHVLATPLFLIDTYLSAENLKTPIFLFIGMIYLFITLGLSIWQFV
                     ITAHIYKFALGTTPVKSVLAAFGLVAVNILTISFWR"
BASE COUNT          416 a          262 c          220 g          335 t
ORIGIN      
        1 cactccccca ttatggagta ttttaaattg tgaaccacta tcgtcaatca gttcggcaac
       61 ccattcggct tctccagata gtgccagtgt ttcaatacgc gaaaattgac ctcgtgatag
      121 aacttcatta agagccttat catctcgagg tttgatagcc acaccacttt tgggaattgt
      181 tcttaataaa taatgaaatt gttgttgaat agctgccaag tccggataaa tatcggcatg
      241 atcaaattcc aaattgttta atactgcaat atttggtcga taatgcataa atttaggtcg
      301 tttatcaaaa aaagcactat cgtactcatc tgcctctatg acaaaccatc tccctttgcc
      361 aaggcaggcg ctggtattga aatcactaga gacgccccca atgagaaatc ccggattcat
      421 tccagcttga tccaaaatcc aggcaatcat tgaagtggta gtcgttttgc catgagttcc
      481 agctaccgca atcacctgat attttgataa aatattttcg gcaagccatt gagggcctga
      541 catatatggt tttcccgact ccataactgc ttcaagaacc ggcattcccc gcttaatagc
      601 attaccgaca ataaccatgt cagattgcaa tgctaaagtg gaatcatcgt agccttcagt
      661 ccatgaaata cctttggcag caagcaaatc actgataggc ggatagcaat tggcatcgct
      721 gcctgtcacc ctatatcctg cgtcgcgggc gagcaaagcc aaggcactca taaaggttcc
      781 acttactcct agtatatgca aatgtgtcat catgtcatcg ccaaaaagaa atagttaaaa
      841 tgttaacagc gactaaacca aaagcagcta aaactgattt taccggtgta gtgcctaaag
      901 caaatttata aatatgggct gtaattacaa attgccaaat agaaagcccc aaagtaatga
      961 acaaatatat catacctata aataaaaaaa tgggggtttt aagattctcc gcagacaaat
     1021 aagtgtcaat taagaacaat ggtgtcgcca aaacatgtat aataatatag gcactcaata
     1081 aacatgttga tgtttgtaca agcctggacg caagattttg aaactttaaa ataacatagg
     1141 tatagaatat aaaagacaaa atcaggctcg ttgccatcat taaacttaat aaccaatcgc
     1201 gagaaaaatc aagttcagcg atattccatt gaa
//
LOCUS       DACTJE010000301         1226 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.303, whole genome shotgun sequence.
ACCESSION   DACTJE010000301 DACTJE010000000
VERSION     DACTJE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.303"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(27..432)
                     /gene="ssrA"
                     /locus_tag="I9760_003042"
     tmRNA           complement(27..432)
                     /gene="ssrA"
                     /locus_tag="I9760_003042"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(583..>1226)
                     /locus_tag="I9760_003043"
     CDS             complement(583..>1226)
                     /locus_tag="I9760_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HAT5044776.1"
                     /translation="LFHEFGHCLHHVLTQVDYLGGSGINGVEWDAVELPSQFFENWCW
                     DEHALSLLTSHADTGETLPSALYERLIAAKNFQSAMAMLRQMEFALFDFRIHQEYQTG
                     KASFVPNILADVRSKTSVVPIVPYNRFQHSFSHIFGGGYAAGYYSYMWAEVLSSDAFA
                     RFEEEGIFNPKTGHDFLKSILEVGGSRKAADAFVEFRGRPATIDALLRHNGIL"
BASE COUNT          337 a          295 c          277 g          317 t
ORIGIN      
        1 gtattggact gtatcggata atgaattggt ggaggcggcg ggaatcgaac ccgcgtccgc
       61 aagtcctctg cctttagctc tacatgcata gccttgtctt ttgaatttaa ctgtggctcc
      121 tccgacgggc aggatgatac acagcgattc cctgagtttc gcgccataac ccgggataga
      181 ttaggacgct agcttatctc ttatgaccgt tgattcgata ccgataagcg ggctcggtcg
      241 aacgggatac tgggtttatt ggccagtaca cagtgatggt ttaactaaag tgcttattag
      301 gcagcgatag cttccccacc agcaaagttt tcatcgtttg catttatatt taattgagtc
      361 ttatttacga gagatctcct tctcggcatg cacttccggt tttgcaaccc acgtcgaagc
      421 caggtcgccc cccattctgt acactttaat tataacataa aatgccggca tgtaagcaaa
      481 atcccgccag cataccgaat atattttcag gacttacgga aaaccccaag gtcaaggcaa
      541 aaaatgtttt accgagagga ttggggggcg caagtcctgt ttttataaaa tcccgttatg
      601 gcgcagcaag gcatcaatcg tcgcgggtct tcctctgaat tcaacaaaag catcggctgc
      661 ttttcttgag ccgcctacct ccagaatgga ttttaaaaag tcatgtccag ttttggggtt
      721 gaaaatacct tcctcctcaa agcgcgcaaa ggcatcgctg gataatactt ctgcccacat
      781 gtaactgtaa taaccagcgg cgtagccacc cccaaaaata tgcgagaagc tgtgctgaaa
      841 gcgattataa ggaacaatgg gtacaacact ggttttagaa cgtacatccg ccagaatatt
      901 tgggacaaat gacgctttac ccgtttgata ttcctggtga atgcgaaaat caaacaaggc
      961 gaattccatt tgtcttaaca tggccatggc ggattgaaaa tttttagcag caatcaagcg
     1021 ctcgtacaat gcggaaggca aggtttcccc tgtatccgcg tgcgaagtca gtaaagacaa
     1081 tgcgtgctca tcccagcacc agttttcgaa aaattggctg ggtaattcca ccgcatccca
     1141 ttccacgcca ttaattcctg agccgccaag gtaatcgact tgagtcaaaa cgtgatgcag
     1201 gcaatgacca aactcgtgaa acaaag
//
LOCUS       DACTJE010000302         1163 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.304, whole genome shotgun sequence.
ACCESSION   DACTJE010000302 DACTJE010000000
VERSION     DACTJE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.304"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..200)
                     /locus_tag="I9760_003044"
     CDS             complement(<1..200)
                     /locus_tag="I9760_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAT5044777.1"
                     /translation="MKIRIKSTRGQCVSTIDEAILSGLAGDGGLFVPDRFPNLTIKKF
                     YEYGNLIDFSVNLLSPFFSDSK"
     gene            complement(372..1136)
                     /locus_tag="I9760_003045"
     CDS             complement(372..1136)
                     /locus_tag="I9760_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HAT5044778.1"
                     /translation="MYLSLPVQSFLLFIFFSFTPLFVVAAEKTQLNFFLRPDMYFGFS
                     VIRDTAHYNFKRNETSQELNFTDFDHFDWYGSGVGGEIYLGYEKFFRTHYYFSLEGFF
                     NRSSNEGKIYFFDSNELYSRILNGAFKQKWQSGLAIHPGIYLPSVGIIYGRVGWIISD
                     VNLSGSITQSGLFGSFSGRFSNTKKKNGVQLGTGLELELTQNWRCRVEWDWNRLQDFT
                     FDTQGKDSNNHRYQTTRIAKHPILEQFKLGLNWRFS"
BASE COUNT          395 a          227 c          176 g          365 t
ORIGIN      
        1 attttactat ctgaaaaaaa tggcgagagt aaattaacag aaaagtcgat taaattaccg
       61 tattcataaa atttttttat tgttaaattg ggaaacctgt caggtacaaa taacccacca
      121 tcccctgcca ggcctgacag tatcgcttcg tctattgtgg agacacattg tcctctcgta
      181 ctctttattc taattttcat ctgcagcctt tgcaccttct gatgaaatat tattaaccag
      241 taagtcactt tcaaagacaa cttttgcttc gcggtatatt gctaacagct atactttagt
      301 caatctaatt tagaagataa tggaagttta gtttattttt tagaaattta ttaagattga
      361 cgtgaatggc atcatgaaaa acgccaattc aaacccaatt tgaattgttc taaaattgga
      421 tgcttggcga ttctggtcgt ttgataccta tgattattag agtccttacc ctgtgtatca
      481 aaagtaaaat cttgcaaccg attccaatcc cactctacac ggcaacgcca attttgagtt
      541 aactccaact cgagacctgt acctaattga actccattct tctttttggt atttgaaaat
      601 cttccactga atgagccaaa aaggccactc tgagtaatgc ttccgcttag gttgacatca
      661 ctgataatcc agcctactcg tccatagatg attcccacag aaggcaaata aataccaggg
      721 tgtatagcta gaccagactg ccatttttgt ttgaatgcac catttaatat tcttgaataa
      781 agttcattgg aatcaaaaaa gtagattttt ccttcattgg aactacggtt aaaaaaacct
      841 tctaaactaa aataataatg tgtccggaaa aatttttcat agcctaaata aatttctcct
      901 ccaactcccg aaccatacca gtcaaaatga tcaaagtcag taaaattaag ctcttgactg
      961 gtttcattcc ttttaaaatt gtaatgggct gtgtctctaa taactgaaaa tccaaaatac
     1021 atatcgggtc ttagaaaaaa attgagctgt gttttttcag ccgctacaac aaataaaggt
     1081 gtaaaactga aaaagataaa caacaagaat gactgaacag gcaaacttaa atacacgaat
     1141 aattcctttt actaagtcaa aaa
//
LOCUS       DACTJE010000303         1136 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.305, whole genome shotgun sequence.
ACCESSION   DACTJE010000303 DACTJE010000000
VERSION     DACTJE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.305"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            46..>1136
                     /locus_tag="I9760_003046"
     CDS             46..>1136
                     /locus_tag="I9760_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISLpn3 family transposase"
                     /protein_id="HAT5044779.1"
                     /translation="MDLAVEDTTAWSEAIFGSVALGDKRLTRRLIQIGKQLSSTPGGS
                     LSGSCGGQDALIEGSYRFLRNKRVTANQIAEGGYQVTSWLSQSIPTLLAIEDTTTLSY
                     THQVKESLGDLGGPKEKSNRGFHVHTTMLMDAEQEKTIGLIAQERWCRDIKERGKKNH
                     RRVRLYTEKESYKWEKNTRELENRLGSKMSDVISVCDREADIFEYIQYKLDHAQRFIV
                     RASHNRKLEGSNCYLFQMLPSAVNLGIYTIEVAQKANRKKRQVTLELKTTSVTFSPSE
                     RRAKARELKPITLNVVIAKEKNPSESDCLEWILLTTEATTTLECTRKITRYYEMRWRI
                     EDFHKAWKSGVGAEEQRMQSIENLEKMIV"
BASE COUNT          402 a          191 c          241 g          302 t
ORIGIN      
        1 ttattccaaa caaacatgat caaatcacct ctttttgagg tatgtatgga cttagcagtt
       61 gaagatacta cagcatggtc ggaagctatt tttggttcag ttgctttagg ggataaacga
      121 cttactcgtc ggttaattca aataggcaaa caattatcat cgacgcctgg tggttctctt
      181 tcaggaagtt gtggagggca ggatgcgctt atagaaggta gttatcgttt tttacgaaac
      241 aaacgagtca cagcgaatca aattgcagag ggtggttatc aagtaacaag ctggttatct
      301 cagtcaatcc ccacgctttt agccattgaa gacacaacca cgctctccta tacccatcaa
      361 gtaaaagaat cattaggtga tttaggcggt cctaaagaaa aaagcaatcg aggttttcat
      421 gttcacacca ccatgctcat ggatgcagaa caagagaaaa caataggatt aatcgcgcaa
      481 gaacgttggt gtcgagatat caaagaaaga ggcaaaaaaa atcatcgacg agtaaggtta
      541 tatacagaga aagaaagcta taaatgggaa aagaatactc gggaattaga gaatcgattg
      601 ggttctaaaa tgtctgatgt gatttctgtt tgcgatagag aagctgatat ttttgaatac
      661 attcagtaca agttagacca tgctcaacgt tttattgttc gagcgagcca taatcgaaaa
      721 ctagaaggaa gtaactgtta tttatttcaa atgttacctt cagcagtaaa cttgggtatt
      781 tatacaattg aagtggctca aaaagcaaat agaaagaaac gccaagtgac tcttgagtta
      841 aaaacaacat ctgttacttt ctcaccttct gaacgaagag ctaaagcacg tgaattaaaa
      901 ccaatcactt taaacgtagt aatcgctaaa gagaaaaatc catctgaaag cgattgcctt
      961 gaatggattc tattaacaac agaagccaca acaacattag agtgtacccg aaaaataacg
     1021 cgatattatg aaatgagatg gcgcattgag gattttcata aagcatggaa atcgggggtt
     1081 ggtgctgaag agcagcgtat gcaatcaatt gaaaatttag agaaaatgat tgtcat
//
LOCUS       DACTJE010000304         1134 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.306, whole genome shotgun sequence.
ACCESSION   DACTJE010000304 DACTJE010000000
VERSION     DACTJE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.306"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..>1134
                     /locus_tag="I9760_003047"
     rRNA            <1..>1134
                     /locus_tag="I9760_003047"
                     /product="23S ribosomal RNA"
BASE COUNT          308 a          226 c          364 g          236 t
ORIGIN      
        1 accttttgta taatgggtca gcgagttact ttcagtggcg aggttaactg aataagggag
       61 ccgtagagaa atcgagtctg aatagggcga tagtcgctgg gagtagaccc gaaaccgggc
      121 gatctagcca tgtgcaggat gaaggttggg taacaccaac tggaggtccg aaccgggtaa
      181 tgttgaaaaa ttatcggatg acgtgtggct aggagtgaaa ggctaatcaa gcccggagat
      241 agctggttct ccccgaaagc tatttaggta gcgcctcgtg aatgattact gggggtagag
      301 cactgtttcg gctagggggc tgtcatggct taccaaaccg atgcaaactc cgaataccgg
      361 ctaattgaat cacgggagac acacggcggg tgctaacgtc cgtcgtggag agggaaacaa
      421 cccagaccgc cagctaaggt ccccaagtac tagttaagtg ggaaacgatg tgggaaggca
      481 tagacagcca ggaggttggc ttagaagcag ccacccttta aagaaagcgt aatagctcac
      541 tggtcgagtc ggcctgcgcg gaagatgtaa cggggctaaa actagtcacc gaagctgcgg
      601 atgtgcgcga aggcgcacgt ggtaggggag cgttctgtag gctgatgaag gtgcattgag
      661 aagtgtgctg gaggtatcag aagtgcgaat gctgacatga gtaacgataa tgtgggtgaa
      721 aagcccacac gccggaagtc ccaggtttcc tgcacgacgt taatcggagc agggtgagtc
      781 ggcccctaag gcgaggctga agagcgtagt cgatgggaac caggttaata ttcctggact
      841 ttttataagt ggtgaagtgg ggacgaagaa ggctaggtga gccaggcgtt ggttgtcctg
      901 gtacttgcat gtagggggga agacttggca aatccggttt tccataactc tgaggtgcga
      961 agtggttctg accttttggt acagagaagt cattgatgcc cggcttccag gaaaagctgc
     1021 tagccataac ttatagagaa ccgtaccgca aaccgacaca ggtggacagg tagagaatac
     1081 taaggcgctt gagagaactc gggtgaagga actaggcaaa atggtaccgt aact
//
LOCUS       DACTJE010000305         1084 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.307, whole genome shotgun sequence.
ACCESSION   DACTJE010000305 DACTJE010000000
VERSION     DACTJE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.307"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..548
                     /locus_tag="I9760_003048"
     CDS             <1..548
                     /locus_tag="I9760_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAT5044780.1"
                     /translation="VLLKWLGETASSLWIDAAVAVSVPFQLNSVADRMNQGFSRLYQA
                     YLLSRLRRIFLKKLNHLNNSPFSKSDLLSLKTFWDFDERITAPINGFKNAHEYYQKAS
                     SRQYLKNITTPTLIIHAQDDPFMTPDAIPASKELSSQTTLELSEYGGHVGFIAGTGKN
                     SSQPVYWLEQRIPEFLLEYLS"
     gene            complement(572..964)
                     /gene="lem12"
                     /locus_tag="I9760_003049"
     CDS             complement(572..964)
                     /gene="lem12"
                     /locus_tag="I9760_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem12"
                     /protein_id="HAT5044781.1"
                     /translation="MQNEKEVKELIQSDKKKRVTWNDNQNEGQIDEKFLKIGTASTPY
                     KNKLDESIAQLMEEGYSEIEATAITGKSVTPAGMVIYNNPVFYKSKESIDIEKANRYN
                     FFSIKNIGLGIVALAAAATVGLVVSKNS"
BASE COUNT          333 a          209 c          174 g          368 t
ORIGIN      
        1 atgttctgct gaaatggttg ggcgagacag catcatcttt atggatagat gcagctgttg
       61 ctgtttcagt tccatttcaa ttaaattcag tggctgacag gatgaatcag ggcttttctc
      121 gcctctatca agcttactta ttaagtcgac tacgtcgtat ttttctaaaa aaattaaatc
      181 acttaaataa ttcaccattt tcaaaaagtg acttactttc cttaaaaacc ttctgggatt
      241 ttgatgagcg aattacagca ccgattaacg gatttaaaaa tgcccacgaa tactaccaaa
      301 aagcaagctc aagacaatac cttaagaata tcaccacacc aactttgatt attcatgcac
      361 aagatgatcc attcatgaca ccagatgcca tcccagcatc gaaggaacta tcttctcaaa
      421 ccactctgga attaagcgaa tatggagggc atgtaggttt tattgccggt acaggaaaaa
      481 actcaagcca acctgtgtat tggcttgaac agcgaattcc tgagtttttg ctagagtatt
      541 tgtcttaaaa atcaattgga atgtattggt tttagctgtt ttttgataca accagtccta
      601 ctgttgcagc ggctgctaaa gccacaatac ctaatccaat atttttaatt gagaaaaagt
      661 tataacgatt cgctttttct atgtctatgc tttccttgga tttataaaaa actggattat
      721 tgtaaataac catacccgct ggtgttaccg attttcccgt tattgctgtt gcttcaattt
      781 ctgaataacc ttcttccatc aattgggcaa tactctcatc caatttgttt ttataaggcg
      841 ttgaagcagt gcctatttta agaaattttt catcaatctg cccttcgttt tgattatcat
      901 tccatgtgac tctttttttc ttatctgatt gaatgagttc ttttacttct ttctcatttt
      961 gcatgcaaac ctctctattg ataaccaaaa tttgagcaat tttaacaaga aatacttaag
     1021 aaaatatgaa ctcaaatgat catttaaaaa tagcgggtaa ttcaaaactc aggtgaaata
     1081 gagt
//
LOCUS       DACTJE010000306         1070 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.308, whole genome shotgun sequence.
ACCESSION   DACTJE010000306 DACTJE010000000
VERSION     DACTJE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.308"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..503)
                     /locus_tag="I9760_003050"
     CDS             complement(<1..503)
                     /locus_tag="I9760_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAT5044782.1"
                     /translation="MKQMAIVLQGGGALGAYELGVLKYLYASANFSPNIISGVSIGAI
                     NAAALIGAKDEPIQTLEAIWEELTVFSAQIFPEMTESFLALLGNAAFYHLRTDYWSMP
                     YWTSFYTTKPLEQLLLKYIDFDKLNNSPIHLILTATNVTTGKITVFENQGNNRTEITP
                     LHVLASG"
     gene            703..942
                     /locus_tag="I9760_003051"
     CDS             703..942
                     /locus_tag="I9760_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAT5044783.1"
                     /translation="MEVERIKNEVKLFLLNSFRIKDIGYSDNIFDTGAMHSLFFIQLL
                     VFIEKKYKIDLEVGEFNPNQLTSIDDIAYFISSKL"
BASE COUNT          341 a          188 c          201 g          340 t
ORIGIN      
        1 ctaccgctgg ccaatacatg caaaggcgtg atttctgtac ggttattacc ttgattctcg
       61 aaaacggtga ttttaccggt tgtaacatta gttgctgtaa gaattaaatg gattgggctg
      121 ttattcagtt tgtcaaaatc aatgtatttt aacaataatt gttcaagggg cttcgtggta
      181 taaaaactgg tccaataagg cattgaccag taatcagttc gcaaatgata aaaagccgca
      241 ttgcctaaga gtgcgagaaa agattccgtc atttcaggga atatctgggc agaaaaaaca
      301 gtcagttctt cccatattgc ttccagagtc tggataggct catctttcgc tccaatcagt
      361 gccgctgcat tgatagcacc aatagaaacc cctgaaataa tgtttggcga aaaattggct
      421 gatgcataga ggtattttaa tacgcccaat tcataagctc ccagtgcccc gcctccctga
      481 agtacaattg ccatttgctt catggatgtc cttatcaatt cgctctgtat cagcaagtat
      541 agcaggtaac cataatgaaa atgatgtcaa gtcatttccc actgttgcgc ttcctgtcta
      601 agtatttgtt aagcattttg actttcctgg aaaaaatatt attatgaata attccattac
      661 ccatatctga ataagactat tcttatgaaa caggaagatt tgatggaagt tgaacgcatt
      721 aaaaatgaag taaaactatt tttactcaat tcttttcgaa ttaaggatat tggttattcg
      781 gataatattt ttgatacagg tgcaatgcac tctttatttt ttattcaatt attagttttt
      841 atagagaaga aatataaaat tgatcttgag gttggtgagt ttaatccgaa ccagttaacc
      901 agcattgatg atatcgctta ctttatttcc agtaaactat aatggtaata ggccaagaac
      961 ctttatgcgg gatttggaac aaacggccag aattgagctt ttacttgtaa tccaatacga
     1021 ataactgtaa taagctttgg tggacaaacg gcatgaaaca aaaaaaacta
//
LOCUS       DACTJE010000307         1021 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.309, whole genome shotgun sequence.
ACCESSION   DACTJE010000307 DACTJE010000000
VERSION     DACTJE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.309"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..>1021
                     /gene="legC1"
                     /locus_tag="I9760_003052"
     CDS             <1..>1021
                     /gene="legC1"
                     /locus_tag="I9760_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC1"
                     /protein_id="HAT5044784.1"
                     /translation="DTLILKNQLNKFGCNVEGVIAGNGEAYNIGGNATRVDKIIEYQS
                     NGISYKRGNQLTLKLKDRVLEHKTNVVETLVRHLKDENFKCLKVDSNQLKAKYPCLIV
                     TFKYDEYRSKPENIPEGYSEFVTHMMIAQINLKLKEKNLSPLIDRRQSFGFLTPTLTD
                     VHTGVRLSLGLTPNKQWIECVEEGIKQTDKSLAKFTLKDKDDLLNLFRAGEFCGDGHK
                     YLYDLYKTGEKTQEEVNKFLQESKVKSECKDIYFSTKDITRIDKDKLKKGVLKRFYYF
                     DSKNGAITLYDNQHQHGSSLKVNADVIHYFLLKNFSSKDLIKLTEVQECWLKEAIDDA
                     QRNNP"
BASE COUNT          402 a          130 c          190 g          299 t
ORIGIN      
        1 aagatacttt aattttaaaa aatcaattaa acaaatttgg ctgtaatgtg gaaggtgtaa
       61 ttgcagggaa tggagaagct tataacatag ggggaaatgc tacacgagta gataagatta
      121 ttgaatatca aagtaacggt atttcttata aaagaggaaa tcaattaacc cttaaattaa
      181 aagatagggt attggaacat aaaacaaatg tggtagaaac cctggttcgg catttaaaag
      241 acgaaaattt taagtgtcta aaggttgata gcaaccaact taaagccaaa tatccttgtt
      301 taattgttac ctttaaatat gatgagtata ggagtaaacc cgaaaatatc cctgaaggat
      361 actctgagtt tgttacccat atgatgattg ctcagattaa tttaaaatta aaagaaaaaa
      421 atctgtcccc attgatagat aggcgacaaa gttttgggtt tctcaccccg acattaaccg
      481 atgttcatac gggtgttaga ttatcacttg gccttacacc aaataaacag tggatagaat
      541 gtgttgaaga aggaataaaa cagacagata agtccttagc aaaatttacg ttgaaagaca
      601 aagatgattt actcaatcta tttagagctg gagagttttg tggggatggg cataaatacc
      661 tttatgactt atataaaaca ggagaaaaaa cacaagaaga agtaaacaag tttctacaag
      721 aatcaaaagt gaaatcagaa tgtaaggaca tttatttttc aacaaaagac ataacgcgta
      781 ttgataaaga taaattaaaa aaaggcgtat tgaaaagatt ttattacttt gatagtaaaa
      841 atggagcaat tactctttat gataaccagc accaacatgg atcttcttta aaagttaatg
      901 cagatgttat tcattatttt cttttaaaaa atttttcctc taaagatttg ataaaattga
      961 ctgaagtaca ggagtgttgg ttaaaggaag caattgatga tgcacagagg aataatccgt
     1021 t
//
LOCUS       DACTJE010000308         1011 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.310, whole genome shotgun sequence.
ACCESSION   DACTJE010000308 DACTJE010000000
VERSION     DACTJE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.310"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..>1011)
                     /locus_tag="I9760_003053"
     CDS             complement(<1..>1011)
                     /locus_tag="I9760_003053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044785.1"
                     /translation="LLKKSKVVLGYLFISVRIVLYKAIIQICNNIISPKTLSNFLLSC
                     YKIIWNKTPQLLPPISKTAYYFYNYLPSLLLENRIQLIVLPEHNLFYFTQLLVYQGRK
                     YQIPAIIVPFTIANTIEWSEAFYNEPSRALSSIPNKIFARAFPHWVNHYKNRSLLLPV
                     ELILLHEMFGITPKNTWLLNSGDIDFLASESEAMKNYYISAGIEEEQIRVTGALYNDE
                     LFFRLQKAEDFRDQLYEKLNMQPGKPMLLCALPPNQCFGRMDLIEFGSYEEIVRFILS
                     KLSQYDKDYNIIVNLHPRIVLDTVSYISDYPVKIYTGNIAEIIPLSSIFVASCSATIR
                     MA"
BASE COUNT          363 a          160 c          160 g          328 t
ORIGIN      
        1 tagccattcg tatggtggct gagcaagagg caacaaaaat agagcttaga ggaattattt
       61 cagcaatatt acctgtatat attttaactg gataatcact aatataactt acagtatcaa
      121 gaacaattct gggatgcaaa tttacaataa tattataatc tttatcatat tgagataatt
      181 tacttaagat aaaacgaacg atttcttcat aagagccaaa ttcaataaga tccatacgac
      241 caaaacactg atttggcgga agcgcacata agagcatagg tttaccaggc tgcatattta
      301 gcttttcata cagttgatct cgaaaatctt ccgctttttg caatctaaaa aaaagctcat
      361 cattatataa tgcccctgta acacgtattt gctcttcttc tattccagca gagatatagt
      421 agtttttcat tgcctcgctt tctgaagcaa ggaaatcaat atcgccacta tttaagagcc
      481 atgtattttt tggtgtgatt ccaaacattt catgtaatag tatcaattcc acaggtagca
      541 ataggctgcg atttttataa tgattaaccc aatgagggaa agcacgggca aaaattttat
      601 tcggtatact gctcaacgct ctggaaggct cattataaaa cgcttctgac cattcgatgg
      661 tatttgctat tgtaaaaggt actataatag caggtatttg gtactttctt ccctgataaa
      721 ctaaaagttg agtaaaataa aatagattat gttcaggcaa aacgatcaac tgaatacgat
      781 tttctaataa caaagacggc aaataattat aaaagtaata tgcggttttt gatataggag
      841 gtaacaattg tggagtttta ttccaaatta ttttatagca acttagtaga aaattagata
      901 atgttttggg agaaataatg ttattacaaa tttgaataat cgctttatat aaaacaattc
      961 taacagatat aaatagatac cctaaaacaa ccttactttt tttcaataaa t
//
LOCUS       DACTJE010000309          985 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.311, whole genome shotgun sequence.
ACCESSION   DACTJE010000309 DACTJE010000000
VERSION     DACTJE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.311"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..64
                     /locus_tag="I9760_003054"
     CDS             <1..64
                     /locus_tag="I9760_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT5044786.1"
                     /translation="RAYGFRNFENYRLRVRVLCA"
     gene            complement(131..203)
                     /locus_tag="I9760_003055"
     tRNA            complement(131..203)
                     /locus_tag="I9760_003055"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Lys,seq:ctt)
     gene            380..>985
                     /locus_tag="I9760_003056"
     CDS             380..>985
                     /locus_tag="I9760_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAT5044787.1"
                     /translation="MLKLFDAHFHIIDYRFPLITNQSYLPPEFTVEDYLQRAKPLNIC
                     GGAVVSGSFQAFDQSYLLAALSILGSDFVGVTQLPATVSDEEIIQLNQKGIRAVRFNV
                     KRGGSENIHRLKYFAHRIYEMVRWHVEIYVDSKDLGDLTSLLLELPAVSIDHLGLSHS
                     GFSQLLHLVEHGIKIKASGFGRVDFDVKKALQTIAQINPDAL"
BASE COUNT          300 a          182 c          184 g          319 t
ORIGIN      
        1 aagagcttat gggtttagaa actttgaaaa ctaccgatta agagttagag tattatgtgc
       61 ttgataaaat gcccccgtat ttgggaaaga cccattttaa tttgtgagga ttgggcgatt
      121 aattattgat tgggccgtat aggattcgaa cctatgaccc tgagattaag agtctcctgc
      181 tctaccaact gagctaacgg cccatattat aaaaaccact tacaatttaa tagcaacaag
      241 tattctatct gtcaactgac aaataaatta gagaatggcc ttatatcatt tatagtcatc
      301 aagcaaaaaa ccaatcgcta ataacgaatg aaatctttgt atgatagtat gattttaacc
      361 gcaaatttaa ttgactaaaa tgctgaaact atttgatgct cattttcata ttatcgatta
      421 tcgttttcct ctaataacta atcaatccta tcttcctccc gaatttactg tcgaagatta
      481 cctccaacgg gcaaagcccc tgaatatttg cggaggagct gttgtttcag gctcattcca
      541 agcctttgac caaagttatc ttctagctgc tctgtctatt ttaggctccg attttgtagg
      601 agttactcaa ttacctgcca ctgtaagtga tgaagaaatt attcaattaa atcaaaaagg
      661 tattcgtgca gtccgcttca atgtcaagcg tggcggctca gaaaacatcc atcggttaaa
      721 gtattttgct catcgtattt atgaaatggt caggtggcat gtagagattt atgttgactc
      781 aaaggatctt ggcgatttga ctagtctgtt attggaatta ccggctgtca gtattgatca
      841 tttaggatta tcgcattcag gtttttctca attactccat ctggtggaac atggcatcaa
      901 aataaaagct tcaggttttg ggagagtcga ttttgatgtt aaaaaagcac tgcaaaccat
      961 tgcccaaatt aatccagacg ctttg
//
LOCUS       DACTJE010000310          948 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.312, whole genome shotgun sequence.
ACCESSION   DACTJE010000310 DACTJE010000000
VERSION     DACTJE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.312"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            378..620
                     /locus_tag="I9760_003057"
     CDS             378..620
                     /locus_tag="I9760_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044788.1"
                     /translation="MTNKILTERLNNELDELGVPCLMTERVQVCSKLFNLPKFKMEAL
                     LNGVIAIDANSIQKIADELEVNKDWLLGGIKKKTKH"
BASE COUNT          315 a          178 c          167 g          288 t
ORIGIN      
        1 attaaaacta ttagaattgg caaaaatcaa taagactaaa agatggcacg atgaattgaa
       61 ttaattttga tagaataggc gtctcgtata atcttattac gttgttttag ggcaagacac
      121 aaacgtttca ggaattgata acttgcttga aaatagataa caaccagact cagtgtttgg
      181 cctgcaatat aatagagcct gttttatcta ctcaagttga tatccctttc aaataaccaa
      241 atcgttcacc gtcagatttt ttctattttt agagaccggg atcatgcttt caattaatta
      301 tctgataaaa attaatttgt taatcaatcc ttaagcttta gtgttttaca ctgatataaa
      361 acgagaagga gaataccatg actaacaaaa tcttaactga acgcttgaac aatgagctgg
      421 atgaacttgg tgttccatgt ctaatgaccg agcgcgtgca agtgtgttcc aaactattta
      481 atttgcctaa attcaaaatg gaagccttgt tgaatggagt cattgctatt gatgccaact
      541 cgatacaaaa aattgccgat gaacttgagg tgaataagga ttggctactg ggcggcatta
      601 agaaaaaaac aaaacactag aggctgttga cattttctcc gaccgctgac gtactgcggc
      661 tttgtccgca gcacccagta ggtttggctc aaaatatcgg tatttataaa gacaccaatc
      721 catacccatg ttgccaattt tagaattgca ttttcctgga ataacaggag tcgatgattt
      781 ttttcgaata gcatcttgaa tctctccact gtacaggctt ttttctaccc ttacataccc
      841 caaaaaaaca gtcttacgat aaattttggc gcaaattaat agtcatgcac tccccctcat
      901 tactacaaaa ttaaaggaaa aagcagacta cttgctggcg tataaatc
//
LOCUS       DACTJE010000311          908 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.313, whole genome shotgun sequence.
ACCESSION   DACTJE010000311 DACTJE010000000
VERSION     DACTJE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.313"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..156
                     /locus_tag="I9760_003058"
     CDS             <1..156
                     /locus_tag="I9760_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT5044789.1"
                     /translation="DLNIKIIQKGIETAEQHEVIKSLGCQYAQGYFYCKPVALNDLLP
                     LIKKTIH"
     gene            297..>908
                     /locus_tag="I9760_003059"
     CDS             297..>908
                     /locus_tag="I9760_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044790.1"
                     /translation="MPFCFSNQPNILNNKLILKIVVLILFFSNSSFAKTINYPWTVSA
                     SLGYVNYQYVHEGDHITATGRFAIGRDLLMKGPFLFGLELGLQSGNQMRLQMTEEEVD
                     ALGGLLVQTTLKPMVDALATIRITPEFTAPLFLQLKAGTAYRQWQMDRDTINDISQLA
                     GEIMAGCGYAITPNASITLSYQGLFGDKPNFTIDLESGIGHVQK"
BASE COUNT          305 a          171 c          160 g          272 t
ORIGIN      
        1 gatcttaata ttaaaatcat tcaaaagggt attgaaactg cagaacagca tgaagtgatt
       61 aaatctttag gatgccaata cgcacaagga tatttttact gcaaacctgt tgccttaaat
      121 gatttactgc cacttataaa aaaaactatc cactaagtaa ttaataattc aacctaattt
      181 tatggcgtca aacgcaataa tcttataaga ttctaaatgt tttgaaccat tacttagaca
      241 gtttttcgtc ataattaatt gatataagaa tcatacattg caatggaagt tagcttatgc
      301 ctttttgttt ttccaaccaa ccaaatattt taaataacaa acttattcta aaaatagttg
      361 tgcttattct cttctttagt aattcgagct ttgcgaaaac aatcaattat ccatggacag
      421 tctccgcaag cttggggtat gtcaattatc aatacgtaca cgaaggcgac catattaccg
      481 caacaggtcg gtttgcaata ggaagagatt tgttgatgaa aggccccttt ttatttggac
      541 ttgaattagg tttacaaagt gggaatcaaa tgcgactgca aatgacggaa gaagaggtcg
      601 atgccctcgg aggactttta gtacaaacca ccttgaaacc tatggttgat gcattagcta
      661 caatccgtat tacccctgaa tttaccgccc ctttgtttct acaactaaaa gcaggaactg
      721 cctacaggca atggcaaatg gatagagaca cgatcaatga catctctcaa cttgcaggcg
      781 aaataatggc tggatgtggt tatgcaatta caccaaatgc cagtattacc ctatcgtatc
      841 aaggcctatt tggagacaaa ccaaatttca ctattgatct ggaaagcggt attggccatg
      901 tacaaaag
//
LOCUS       DACTJE010000312          869 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.314, whole genome shotgun sequence.
ACCESSION   DACTJE010000312 DACTJE010000000
VERSION     DACTJE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.314"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            56..>869
                     /locus_tag="I9760_003060"
     CDS             56..>869
                     /locus_tag="I9760_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT5044791.1"
                     /translation="MSLLLDDIKYFIAASDTLNMTRASEIIGISQPALSYSIKRLENK
                     LGGLLFIRLKNGIQLTKLGEEFKRRSHRLLYEWEQVQNIANPESGFVQGNYTIALHPS
                     VALYTLQYFMPKLQVNYPGLGINFIHGHSREMTEKVISWEADFGIVVNPIQHPDLVII
                     KLSTDEVTVFYANDAQNKLIYDRKLAQSQYILKKIGKKITFNGVINSTSLEVVAKLTA
                     LGLGYGILPTRVTYPYKHLKKVLNAPVFRDEICLVYRPEKHNNPVSKKIIQII"
BASE COUNT          281 a          164 c          161 g          263 t
ORIGIN      
        1 ttattttaat tattgtgata aattttactt atacctaaag tgaagttggt gagtcatgtc
       61 tttgttgttg gatgatataa agtatttcat agcggccagc gatacgctca atatgacaag
      121 agcttctgaa attattggaa tatctcaacc tgcgcttagt tattctataa agagattgga
      181 gaacaaactt ggtggattac tatttatcag gcttaaaaac ggaatccagc ttacaaaact
      241 tggagaggag tttaagaggc gctcccaccg tctattatat gaatgggagc aggtgcaaaa
      301 catagctaac cctgaatcag gttttgttca gggaaactat actatagccc ttcacccttc
      361 tgttgcgctt tatactctcc agtactttat gccaaaactg caagtgaact atcctgggct
      421 tggcattaat ttcattcatg gtcattcaag agagatgact gaaaaagtga taagttggga
      481 agccgatttt ggtattgttg tgaatcccat acaacaccct gatctcgtta taataaagct
      541 gtctacggat gaagtgacag tcttttatgc caatgatgcg caaaataaac tgatttatga
      601 tagaaaactt gcccaatcac aatacatcct taaaaaaatt ggcaaaaaaa taactttcaa
      661 tggcgtaatt aattctacca gccttgaagt ggtagccaaa ctcacagcct taggtcttgg
      721 gtatggcatt ctacctacca gggtcaccta tccctataaa cacctcaaaa aagtactcaa
      781 tgcgcctgtg tttagagatg agatttgtct tgtctaccga cctgaaaaac ataataatcc
      841 tgtcagtaaa aaaatcattc aaattatca
//
LOCUS       DACTJE010000313          841 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.315, whole genome shotgun sequence.
ACCESSION   DACTJE010000313 DACTJE010000000
VERSION     DACTJE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.315"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(60..>841)
                     /locus_tag="I9760_003061"
     CDS             complement(60..>841)
                     /locus_tag="I9760_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT5044792.1"
                     /translation="GKEGINPYVYQWVNNARKRKSLKDAQKLMVEAGYPRGIDPKTGK
                     HLILHYDVMTTGGPDDKARLDWMRKQFANIGIDLNIRATLYNRFQEKMRIGHAQIFSW
                     GWNADYPDPENFLFLLYGKNGKVKYGGENASNYENPEFDRLFNLMKNRPNDLQRQKLI
                     DKMLAIVRYDAPWVWGMHSEDFILSQDWVSNIKSNTISLNTLKYVSVNVPERNQLRRS
                     WNQPVFWPLGVLFGLLIALVIPLLITYLKKEKQPAERVKLK"
BASE COUNT          256 a          184 c          125 g          276 t
ORIGIN      
        1 aagattaata ccaaacaaaa ttggaatcgc atataaaatt ctgcggagta tgtatttaat
       61 cattttaatt ttactctttc tgccggctgt ttttctttct ttaaataagt aatcaataaa
      121 gggattacca gagcaatcaa taggccaaac agcacaccta aaggccaaaa taccggttga
      181 ttccaggatc ttcgcagttg gttacgttca ggtacattaa cagaaacata ttttagagta
      241 ttgagagaaa tcgtattgga ttttatatta gatacccaat cttgcgataa aataaagtct
      301 tcagaatgca tgccccatac ccatggagca tcataccgga caatcgctaa catcttatca
      361 atcagttttt gtctttgtag atcgttaggg cgatttttca tcaaattaaa caatctatca
      421 aattcaggat tttcataatt tgatgcattt tcaccaccat acttcacttt tccatttttc
      481 ccataaagca aaaataaaaa attttcggga tcaggataat cggcattcca gccccaacta
      541 aatatttgag catgacctat ccgcatcttt tcctggaaac gattgtaaag agtcgcccta
      601 atattcaaat ctatccctat atttgcaaat tgctttcgca tccagtcaag ccttgcttta
      661 tcatcaggtc cacctgtcgt catgacatca taatgaagaa tcaggtgttt cccagttttc
      721 ggatctatcc ctctcggata tcctgcctca accattaact tctgcgcgtc ctttaaggat
      781 ttgcgtttcc tcgcattatt cacccattga taaacatagg gattaatacc ctcttttcct
      841 t
//
LOCUS       DACTJE010000314          821 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.316, whole genome shotgun sequence.
ACCESSION   DACTJE010000314 DACTJE010000000
VERSION     DACTJE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.316"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            112..227
                     /gene="rrf"
                     /locus_tag="I9760_003062"
     rRNA            112..227
                     /gene="rrf"
                     /locus_tag="I9760_003062"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            334..409
                     /locus_tag="I9760_003063"
     tRNA            334..409
                     /locus_tag="I9760_003063"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:367..369,aa:Thr,seq:tgt)
     gene            482..566
                     /locus_tag="I9760_003064"
     tRNA            482..566
                     /locus_tag="I9760_003064"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:516..518,aa:Tyr,seq:gta)
     gene            587..660
                     /locus_tag="I9760_003065"
     tRNA            587..660
                     /locus_tag="I9760_003065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Gly,seq:tcc)
     gene            713..788
                     /locus_tag="I9760_003066"
     tRNA            713..788
                     /locus_tag="I9760_003066"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:746..748,aa:Thr,seq:ggt)
BASE COUNT          239 a          166 c          190 g          226 t
ORIGIN      
        1 acttcagaat atgatattga tttgtatacc tgataagtat cgtgtaaact ctgactcttt
       61 accaaacctg tggcttaata aagcaatcaa agcctcaggt aaaccagttt tcctggcgac
      121 tatagcgatt tggaaccacc tgataccatc tcgaactcag aagtgaaaca tttccgcgcc
      181 aatgatagtg tgaggcttcc tcatgcgaaa gtaggtcatc gccagggttt tattttatag
      241 tgtggcatgt aaagccgact aaattaaaat aggttagttg tatacgcata atataaatta
      301 aataagcgct aacctatcat atgtaaagaa tatgctggcg tagctcagtt ggtagagcaa
      361 ctgacttgta atcagtaggt cccgggttcg attcctggtg ccagcaccat ctaaataggg
      421 ctaaacgttg actaaaaatg ttgacaatcc attcatctta aaagacaata gcgttctttt
      481 tggaggggtt cccgagcggt caaagggatc agactgtaaa tctgacggct cagccttcga
      541 aggttcgaat ccttccccct ccaccagtta agagagaagt tagaaagcgg gtgtagttca
      601 atggtagaac ttcagccttc caagctgata gcgtgggttc gattcccatc acccgctcca
      661 aagtagtgaa tagtatttgc ttgttggtgt gagttagcaa gctgatatga aagcccacat
      721 agctcaggcg gtagagcact tccttggtaa ggaagaggtc gttggttcga gtccagttgt
      781 gggcaccatg taatataaat tagcaaacgg ggagactttc c
//
LOCUS       DACTJE010000315          821 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.317, whole genome shotgun sequence.
ACCESSION   DACTJE010000315 DACTJE010000000
VERSION     DACTJE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.317"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            91..471
                     /locus_tag="I9760_003067"
     CDS             91..471
                     /locus_tag="I9760_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044793.1"
                     /translation="MFRYFKQGWHGDLKFSEVLFGSGGDYFLLEGGLAYIGFYILFAI
                     LLITSKPLSLDNILALALFSYGIVLYIWLIKAFWGSAHHCSNKISAVLIRTFTILLPL
                     ISIVLFFLIIIYYLVTAITDALSG"
     gene            525..>821
                     /locus_tag="I9760_003068"
     CDS             525..>821
                     /locus_tag="I9760_003068"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444610.1"
                     /note="catalyzes the transfer of a methylene group from
                     S-adenosyl-L-methionine to the cis double bond of an
                     unsaturated fatty acid chain resulting in the replacement
                     of the double bond with a methylene bridge; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase"
                     /protein_id="HAT5044794.1"
                     /translation="MDIKKNKAKIFVNYMLQLANININGQTPWDIQIYNDEFYSRLLR
                     DADLGLGESYMDGWWDCQRIDLFISKLINANLESKIKINFKLAFNVFLSKILNLQ"
BASE COUNT          241 a          121 c          148 g          311 t
ORIGIN      
        1 tgggggtttt agtaagtcct gaaaaattct ttcatccatt gtcctagctt ggcttataat
       61 aaaaattata aataacataa caggatcggt atgtttcgtt attttaaaca gggatggcat
      121 ggagatctga aattttctga agttctgttt ggttcaggtg gtgattattt tttgcttgaa
      181 ggcggcctgg cgtatattgg attctatatt ttatttgcca ttttgttaat aacatctaaa
      241 ccattatctc tagataatat attagcccta gctctcttct cctatggcat agtcttatac
      301 atctggttaa ttaaagcatt ttggggatcg gcgcatcatt gctcaaataa aatttcagcc
      361 gttttgattc gtacctttac cattcttttg cccttgattt cgattgtgtt gttttttctg
      421 attattattt attatcttgt tacagccata acagacgctt tgagtggctg atgatgttgc
      481 attgattgaa tgtttcaaca tcctttataa aaagggagtt tgatatggat attaaaaaaa
      541 acaaagcaaa aatatttgtt aattacatgc ttcagctagc caatatcaat atcaatggcc
      601 aaacaccttg ggatatacaa atttataatg atgagttcta ttccagatta ttgcgtgatg
      661 ccgatttggg tctgggtgaa agttacatgg acggttggtg ggattgtcaa cgaattgatt
      721 tatttatttc aaaactgatt aacgcaaatc ttgagtccaa aattaaaatt aattttaaat
      781 tggcatttaa tgtcttttta tcaaaaatat tgaacctgca a
//
LOCUS       DACTJE010000316          805 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.318, whole genome shotgun sequence.
ACCESSION   DACTJE010000316 DACTJE010000000
VERSION     DACTJE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.318"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..>805
                     /locus_tag="I9760_003069"
     CDS             <1..>805
                     /locus_tag="I9760_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2745 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044795.1"
                     /translation="WDFLYKKTFPRMQSRGEIPLEVLPHLLMLIESYYHLKNSGADFK
                     LFTAEIHKFFKILYQFKLENINFLYGVKILYHGKEYYLLELFVLINMAQSYDVAEQLK
                     AIMSWLYQFNPILKASNKGLLSFYAELEPKFHSEGHLEKRVETGTDNLLYRIKTFLVS
                     LFVTPFEVFPFSGKTISFWDIKNVIFSEGQEIYNQFAPFIMTNKLDALIAVYKKTMDE
                     HIILCQKNKHICKWLTHYQSTLDWYQLVEAGDLSKMDVYWFDPELLFHVL"
BASE COUNT          276 a          112 c          135 g          282 t
ORIGIN      
        1 tgggattttc tatacaaaaa aacttttccc cgaatgcaat caagaggtga aattccatta
       61 gaagtacttc ctcacttgct tatgttaatt gagagttatt atcatttaaa aaattctgga
      121 gcagatttta aattatttac agcagagatt cacaaatttt ttaaaatatt atatcagttt
      181 aaattagaaa atattaattt tctctatgga gttaaaattc tatatcatgg gaaagaatac
      241 tatctattag aactttttgt tttaatcaat atggcgcaat cttatgatgt ggctgaacag
      301 ttaaaagcta taatgagttg gctgtatcaa tttaatccca tattaaaggc atcaaataaa
      361 gggttattat ctttttatgc agaattggaa ccaaaattcc actcggaagg tcacttagag
      421 aagagagtag aaactgggac agacaattta ttgtatcgta ttaaaacttt tttagtgtca
      481 ttatttgtta ccccattcga agtttttcct ttttcaggta aaacaatcag tttttgggat
      541 atcaaaaatg taatcttttc agaaggacaa gagatctata atcagtttgc tcctttcata
      601 atgacgaata agttagacgc tttaattgca gtctataaaa agacaatgga tgagcacatt
      661 atcctttgcc aaaaaaataa acacatttgt aaatggctga cacactatca atcaacgctg
      721 gattggtatc agctggtaga ggcgggtgat ttatctaaaa tggatgttta ttggtttgac
      781 ccggaattat tatttcatgt tttag
//
LOCUS       DACTJE010000317          779 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.319, whole genome shotgun sequence.
ACCESSION   DACTJE010000317 DACTJE010000000
VERSION     DACTJE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.319"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..>779
                     /locus_tag="I9760_003070"
     CDS             <1..>779
                     /locus_tag="I9760_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAT5044796.1"
                     /translation="LDLTYNRLPRCSHCSSKKVRKKSSYERKVHHELIGHRRTILRFK
                     AYKLFCNECGRYGNQQFPGIAKYQRSTERLQVQIFHHHTSGISQKELSLQFKQGKATI
                     ERWYHRRYQIEGNELLNTPCPKVLGLDEHFFSRKHGFATTLCDLKKHKVFDIVKGRRE
                     VDLNHYLNSLKGKDRVQVVCMDLSNTYRSLVKKHFPNAKIVADRFHVIRLVQHQCMMT
                     YRELSSSIKNNRGLLALLRTKPDKLTSHKKIKRDTFLAENP"
BASE COUNT          265 a          147 c          143 g          224 t
ORIGIN      
        1 attggattta acctataacc gtttaccacg ctgttctcat tgttcaagca aaaaggttcg
       61 aaagaaatcg agttatgaac gaaaggttca tcatgaatta ataggtcaca gacggactat
      121 tcttcgattc aaagcataca aactgttttg caatgaatgc ggtcgttatg gtaatcaaca
      181 atttcccgga attgctaaat atcaacggtc tacagagcgt ttacaagttc aaatatttca
      241 tcaccatacg agtggtattt cccagaaaga gttatctctt caatttaaac aagggaaagc
      301 aaccattgaa cgttggtatc atagacgcta tcaaatagaa ggaaatgaat tgctcaatac
      361 gccttgtcca aaagtgttag gtcttgatga gcacttcttt agtcgaaaac atggctttgc
      421 aactacctta tgtgacctta aaaagcataa agtgtttgat atcgttaaag gacgaagaga
      481 ggttgattta aatcactacc ttaactcttt aaaagggaaa gaccgtgttc aagtggtctg
      541 tatggattta agtaatacct accgctctct ggtcaaaaag catttcccaa acgctaaaat
      601 agtagctgat aggtttcacg tcattcgatt agtacaacat caatgtatga tgacttatcg
      661 tgagttatcc agctcaatta aaaataaccg aggtctattg gctttgttgc gaaccaaacc
      721 agataaactc acatctcata agaaaataaa gcgagacact ttcctcgctg aaaatccag
//
LOCUS       DACTJE010000318          755 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.320, whole genome shotgun sequence.
ACCESSION   DACTJE010000318 DACTJE010000000
VERSION     DACTJE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.320"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..>755
                     /locus_tag="I9760_003071"
     CDS             <1..>755
                     /locus_tag="I9760_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044797.1"
                     /translation="NIQYEGKILREIKTSLSLAIRQLLDKSIPGYDPNADDFINKLCN
                     ELLHIKRITPKDLDYMQIKELKKSVEAEPTVKNILSYNIQLKALKAYVEHSDTLLNSE
                     KKRKQAQKNYIKLIQLAKSMGLLAFDPNKPRHNQLLQTVKKNKANSFAKLLTKNANYD
                     NQLLKDFLNNFNKILSLSIIIIIIGSSTYEFGYFFNLGLRINDLPVGYTDLFFISLSW
                     APKIIFAVFIIMLLEIYYYRKSVTQGLSYSLLD"
BASE COUNT          298 a           98 c          108 g          251 t
ORIGIN      
        1 aacattcagt atgaaggaaa aattttaaga gaaattaaaa cttctttgtc tttagctatt
       61 aggcagttac tagataaaag cataccaggc tatgatccga acgctgatga ttttataaat
      121 aagctatgca atgagctact acatataaaa cgaattaccc ccaaagattt agattatatg
      181 caaattaaag aactaaaaaa aagtgtggaa gcagagccga cagttaaaaa tatattatct
      241 tataatattc agttaaaggc tttaaaagct tatgtagaac atagcgacac tctattgaat
      301 agtgaaaaaa aaaggaagca agcacagaaa aattacataa aacttatcca attagctaaa
      361 tccatgggtt tattagcatt tgatcctaac aaaccacgtc ataatcagct attacaaaca
      421 gtgaaaaaaa ataaagctaa tagttttgca aagcttctaa caaaaaatgc taattatgat
      481 aatcaattgt taaaagattt tttaaataat tttaataaaa tattgtcact cagtattatt
      541 attataatta ttggctcatc tacatatgaa tttggatatt tttttaattt aggattgagg
      601 ataaatgacc tgcctgttgg ctatacagat ttattcttta tttctttaag ttgggctcct
      661 aagataatat tcgcagtgtt tataataatg cttttagaaa tatattatta tcggaaatct
      721 gttacgcaag gtctaagtta ttctttatta gataa
//
LOCUS       DACTJE010000319          755 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.321, whole genome shotgun sequence.
ACCESSION   DACTJE010000319 DACTJE010000000
VERSION     DACTJE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.321"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..>755
                     /locus_tag="I9760_003072"
     CDS             <1..>755
                     /locus_tag="I9760_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044798.1"
                     /translation="VTVPCERTAWDIGITALYLQPIYDADWGYNGFTQVGGWRHWHDV
                     DHEWDWGFKLEGSYHFNTGNDINVNWYHFDNDSDHWFDFANWHNYNNKWDAVNAELGQ
                     FVDFSANKKMRFHGGVQYARIEADVNRYFNNFAFNGFNSKFNGFGPRTGLDMNYVFGN
                     GFGIYAKGAAAILVGTSDFYDGIGFVTGSKNAIVPELEAKLGADYTYAMAQGDLTLDV
                     GYMWFNYFNAMHNTATNGLETDFAASGPYIGLK"
BASE COUNT          194 a          143 c          177 g          241 t
ORIGIN      
        1 atgtaactgt tccttgcgaa agaactgcat gggatattgg tatcaccgca ctatatttgc
       61 aaccaatcta tgatgctgat tggggctaca atggtttcac ccaagttggt ggctggcggc
      121 attggcatga tgttgaccat gagtgggatt ggggcttcaa attagaaggt tcttatcact
      181 tcaatactgg taatgacatc aatgtgaact ggtatcattt tgataacgac agtgatcact
      241 ggtttgattt tgctaactgg cacaactaca acaacaagtg ggatgcagtt aatgctgaac
      301 taggtcaatt cgtagatttc agcgctaaca agaaaatgcg tttccatggc ggtgttcaat
      361 atgctcgcat tgaagctgat gtgaaccgtt atttcaataa ctttgcgttt aacggattca
      421 actccaagtt caacggcttt ggtcctcgca ctggtttaga catgaactat gtatttggca
      481 atggctttgg tatttatgct aaaggagctg ctgctattct ggttggtacc agcgatttct
      541 atgatggaat aggtttcgtt actggttcta aaaatgccat cgttcctgag ttggaagcta
      601 agcttggcgc tgactacact tacgcaatgg ctcaaggcga tttgacttta gacgttggtt
      661 acatgtggtt taactacttc aacgctatgc acaatactgc tactaatggt cttgaaactg
      721 atttcgcagc ttctggtcct tacattggct tgaag
//
LOCUS       DACTJE010000320          743 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.322, whole genome shotgun sequence.
ACCESSION   DACTJE010000320 DACTJE010000000
VERSION     DACTJE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.322"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            197..>743
                     /locus_tag="I9760_003073"
     CDS             197..>743
                     /locus_tag="I9760_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044799.1"
                     /translation="MAKKRLLIIGPVSTQSSSLRHLSQNLSFLNPYYSIECLDPLVKQ
                     HECPDEEYYGIWQKQLKQLIKIYDAFVGFSFGGVILQQCFSILNGEKKQIILFSTPTF
                     SDSSLREKLEKVISLCSQNKLEEALSTLYQAVYAPHPIPVTSLRYLNRELAIKRLIFG
                     LTKVLKTDCVSILNHCNVNHFH"
BASE COUNT          239 a          144 c          121 g          239 t
ORIGIN      
        1 gggaatttct gatctgtcta cagcagacct gaaaagctat aaaccaggca agctctagaa
       61 aatgcttata tttccaatat aggaatataa ggctgggaca ccttgattcc ccaagatttg
      121 atgctccaga aagcaacgta ctttcaaaat taagctggca aaatcataga ccttccagga
      181 ataagtagag aatacaatgg caaaaaaaag attattgatt atcggtcctg tttcaactca
      241 atcctctagt ctgaggcact taagccaaaa tttatctttt cttaatcctt attactccat
      301 tgaatgtctt gatcctcttg tcaagcagca tgagtgtccc gatgaagaat attatggaat
      361 ttggcaaaag caactaaaac aactcatcaa aatttatgat gcatttgttg gcttttcttt
      421 tggtggtgtt attcttcaac aatgtttttc catattgaac ggggaaaaaa aacaaattat
      481 tcttttttct acacccacat tttcagattc ctccttaaga gaaaaacttg aaaaagtcat
      541 ctcgctctgt tcgcaaaata aactggaaga agcattatcc acgctttatc aagcagttta
      601 tgctcctcat cccattcctg tcacttcgct gcggtattta aatagggaac ttgcgatcaa
      661 gcgacttatt tttggcttaa ccaaagtatt gaaaacagat tgtgtttcga ttttaaatca
      721 ttgtaatgtg aatcactttc att
//
LOCUS       DACTJE010000321          715 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.323, whole genome shotgun sequence.
ACCESSION   DACTJE010000321 DACTJE010000000
VERSION     DACTJE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.323"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..>715)
                     /locus_tag="I9760_003074"
     rRNA            complement(<1..>715)
                     /locus_tag="I9760_003074"
                     /product="23S ribosomal RNA"
BASE COUNT          160 a          225 c          156 g          174 t
ORIGIN      
        1 caatcagcca attagtacaa gttagcttca cgcattactg cgcttccaca ccttgcctat
       61 caacgtcgta gtcttcaacg ggcttcatgg gaaaactcat cttgagggag gcttcccgct
      121 tagatgcttt cagcggttat cccgtccgaa cttagctacc cggccatgcc actggcgtga
      181 caaccggtgc accagaggtt cgtccactcc ggtcctctcg tactaggagc agctcctctc
      241 aattttccta cgcccacggc agatagggac cgaactgtct cacgacgttc taaacccagc
      301 tcgcgtacca ctttaaatgg cgaacagcca tacccttggg acctgcttca gccccaggat
      361 gtgatgagcc gacatcgagg tgccaaacac cgccgtcgat atgaactctt gggcggtatc
      421 agcctgttat ccccggagta ccttttatcc gttgagcgat ggcccttcca ttcagaacca
      481 ccggatcact aagaccaact ttcgttcctg ctcgagccgt cactctcgca gtcaagcacc
      541 cttttgcctt tacactcttg gtacgatgtc cgaccgtacc gagggtacct ttgtgctcct
      601 ccgttactct ttgggaggag accgccccag tcaaactacc catcatacac tgtcctcatc
      661 caggattact ggaccaagtt agaacctcaa taacaacagg gtggtatttc aaggg
//
LOCUS       DACTJE010000322          699 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.324, whole genome shotgun sequence.
ACCESSION   DACTJE010000322 DACTJE010000000
VERSION     DACTJE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.324"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..530)
                     /locus_tag="I9760_003075"
     CDS             complement(<1..530)
                     /locus_tag="I9760_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2745 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044800.1"
                     /translation="MLVGSIWFIKDRLQTLMLVQDIRRFLDELRESKTILPCDKRLSA
                     ILQEHFSHRENHEELTSNDIQFVLQCFSERWIADSECDYLLYPSQANQVWIKLAHEIE
                     PFTDKNYLQILLPHVTNQFDFNNLTPLTETVRLENFYLGYDGKTLYRKRGLCEHLLDN
                     QFELSTCRTLKTKQCEV"
BASE COUNT          250 a          121 c          109 g          219 t
ORIGIN      
        1 acttcacatt gtttagtctt tagtgttcta caagtggata actcaaattg attatcaagc
       61 aaatgttcac ataatcctct ttttctatat aaagtttttc catcatatcc aagataaaaa
      121 ttttccaatc gaacggtttc agtcaatgga gttaaattat taaaatcaaa ctgattggtt
      181 acgtggggca gcaaaatttg caaataattt ttatctgtga atggttctat ctcatgagcc
      241 aacttgatcc aaacctggtt tgcctgagaa ggataaagca gataatcaca ctctgagtca
      301 gcaatccagc gttcgctaaa acattgcaaa acaaattgaa tatcattgga ggttaactct
      361 tcatgatttt ctctatgcga gaaatgttct tgtaaaatag cagataatct tttatcacag
      421 ggaagaatag ttttcgattc cctcaactca tcaagaaacc tacgaatgtc ttgtactaac
      481 attaaagtct gcagcctatc cttaataaac caaattgaac caacaagcat tctctatcaa
      541 tactattgaa ctggaatcac ctaaacaatg tcatgcttgc taataaatag gaattatttt
      601 gatatcagat tgtactttgg tcaatagagg atttaattaa tagaaaaaaa catgtgaaaa
      661 taaaagaagc ggcttgagag ccgctataag gaataaaac
//
LOCUS       DACTJE010000323          694 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.325, whole genome shotgun sequence.
ACCESSION   DACTJE010000323 DACTJE010000000
VERSION     DACTJE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.325"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            49..>694
                     /locus_tag="I9760_003076"
     CDS             49..>694
                     /locus_tag="I9760_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2844 family Dot/Icm T4SS effector"
                     /protein_id="HAT5044801.1"
                     /translation="MAETPKPKSQTPTPKPKPKPTDEQTVEPAPQQSTLGKVGSVIGG
                     LAKKGLGKLADNIGKQFGSNAPQPGAENKPAEGQKKDDDKKDPQMEMIERYMKLVEQW
                     QGGINKPFYWVGGKISAGASFLVDKAVEGIKNKLAESEQPKPNPNSSTQQSNSSSSSS
                     SSSTTPTPTSSSSSGSQSSSPPTTSSGSDSQSSSPPTTSSGSDSQSSSPPTDSSS"
BASE COUNT          236 a          166 c          156 g          136 t
ORIGIN      
        1 cttgatgagg tactataatt tagatatcaa cagatatggt aaattaacat ggcagaaact
       61 ccaaaaccca aatcacagac accaacaccg aagccgaaac caaaaccaac ggatgagcag
      121 actgttgagc cagctcctca acaaagtacg cttgggaaag taggtagtgt aatcggagga
      181 cttgctaaaa aagggttagg caaattagcc gataatatcg gcaagcaatt tggctcaaat
      241 gctcctcagc ctggtgctga aaataagcct gcggaaggac aaaagaagga tgatgacaaa
      301 aaagatccgc aaatggaaat gattgaaagg tatatgaagc tggtcgagca atggcaaggc
      361 ggtatcaaca agccttttta ctgggtgggt ggcaaaatat cagctggtgc cagttttctg
      421 gttgataagg cagtggaagg gattaaaaat aaactggctg aatcagagca acccaaacca
      481 aatcccaact cctcaacgca acaatccaat tccagttcga gttccagttc tagtagtact
      541 acccccacac ctacttcgtc aagcagcagc ggcagccagt cttcatcacc gcctacaaca
      601 agcagtggca gcgacagcca gtcttcatca ccgcctacaa caagcagtgg cagcgacagt
      661 cagtcttcat caccgcctac agacagtagc agca
//
LOCUS       DACTJE010000324          683 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.326, whole genome shotgun sequence.
ACCESSION   DACTJE010000324 DACTJE010000000
VERSION     DACTJE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.326"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(59..607)
                     /locus_tag="I9760_003077"
     CDS             complement(59..607)
                     /locus_tag="I9760_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT5044802.1"
                     /translation="MKTKLPDNHLKLIGSKVFLLPVTLADCTDNYLKWLSDPSVNHYL
                     ETRWSIQDMESIRNFVSNMRTDPNNYLFAIRELKTEQHIGNIKLGPINTNHSYADVSY
                     FIGEKNAWGKGFATEAIQLVTHFAFEQLHLHRLQAGLYASNIGSGKALEKAGYHLEAV
                     FKKQLKLDSNWEDHLWYAIFRE"
BASE COUNT          213 a          118 c          102 g          250 t
ORIGIN      
        1 aatacttggt aagtaatcct tggatttcag cctaagagtt tatcataata tttatgattt
       61 attccctaaa aatagcgtac caaagatgat cttcccaatt ggaatctaat tttaattgtt
      121 ttttgaatac cgcctccaga tgatagcctg ctttttctaa agctttcccg ctaccaatat
      181 tgctggcata taaacctgct tgaagtcgat gtaaatgtaa ttgttcaaat gcaaagtgag
      241 tcaccagttg tattgcttca gtagcaaatc ctttccccca tgcatttttc tcaccaataa
      301 aataactcac atctgcataa ctatgatttg tattaattgg acctaatttt atattaccta
      361 tgtgttgttc ggtttttaat tctcggatag cgaacaggta attattagga tctgttctca
      421 tgttacttac aaaatttcga attgattcca tgtcttgaat agaccatctt gtttctaaat
      481 aatgattaac acttggatca cttaaccatt taagatagtt atcagtgcag tctgcaagag
      541 ttacaggcaa taaaaaaacc ttacttccaa taagttttag atggttatct ggtaattttg
      601 ttttcataaa attaaaccac tttgaaatgt aattggtaat tacttaaaaa tgctttggcg
      661 cgaatcaagt tactgtctgt aaa
//
LOCUS       DACTJE010000325          677 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.327, whole genome shotgun sequence.
ACCESSION   DACTJE010000325 DACTJE010000000
VERSION     DACTJE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.327"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            10..>677
                     /locus_tag="I9760_003078"
     CDS             10..>677
                     /locus_tag="I9760_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019349747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAT5044803.1"
                     /translation="MHYIQINNPGPHSELTLVEGPNPGFNESQILVRVKATALNRADL
                     MQKYGKYPPPPGESDIPGLEVAGEVIVTGSKVKQFKPGDKIYGLVGSGGYAEICPVDA
                     SLAHHIPSHWNYSLAAALPEALTTVYATLYDLGALSAGQTLLIHGAGSGIASLAIQMA
                     KLTNTKVITTVGDSSKIEKALALGADQVIHYKEQDFELLIEDNSVDLIIDFVGGDYFN
                     KHLH"
BASE COUNT          207 a          132 c          136 g          202 t
ORIGIN      
        1 gatcttatct tgcattacat acagattaat aatccaggac ctcatagtga attaaccctt
       61 gtcgaaggtc caaatcctgg ttttaatgaa tctcaaattc ttgtgcgtgt caaagccaca
      121 gccttgaata gagctgattt gatgcaaaaa tatggtaaat atccccctcc tccaggagaa
      181 tccgatatac ctggactaga agtagctgga gaagtaatcg tcactggttc caaggtaaaa
      241 caatttaaac cgggtgataa aatttacggt cttgtcggga gtggaggtta tgctgaaatc
      301 tgtccagtag acgcttcttt agctcatcat attccttctc attggaatta ttcattggct
      361 gcagcgctcc cggaagcatt aacgacagtc tatgctacat tgtacgattt aggtgcctta
      421 agtgctggac aaacattatt gatccatggc gctggaagcg gaatagcttc attagccata
      481 caaatggcta aattaacaaa cactaaagtg ataaccacag tcggtgattc cagtaaaata
      541 gaaaaggctc ttgccctggg cgccgatcaa gtcattcatt acaaagaaca agattttgaa
      601 ttattaattg aggacaattc tgttgatttg atcatcgatt ttgtcggtgg tgattatttt
      661 aacaaacact tacattt
//
LOCUS       DACTJE010000326          672 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.328, whole genome shotgun sequence.
ACCESSION   DACTJE010000326 DACTJE010000000
VERSION     DACTJE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.328"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..>672)
                     /locus_tag="I9760_003079"
     CDS             complement(<1..>672)
                     /locus_tag="I9760_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044804.1"
                     /translation="IAQPTACVNSLFTATGNQHWKSIVLKLTNNCNQAVDFQNSTVSF
                     QTTAALNTSFWGDFSPLSYPDNALNISSQPQSGGNYLATLNLHFPSYPGANSKLPVGS
                     SISIKYGAVTDSHIEGTANVYLSTTVETGSIQLINAAAKPTNVSQGYALVHVTMNGQS
                     VSDVQLPWETSKTLSGFAAGNYAISAETVTDSNGNLYLGQANPSMVNVIANQTTSSTI
                     NYARV"
BASE COUNT          198 a          125 c          142 g          207 t
ORIGIN      
        1 tgtacccgag cataattaat agtcgagctc gttgtttggt ttgcaatcac attcaccatg
       61 ctgggattgg cctgtcccag ataaagattg ccattactgt cagtaaccgt ttcagcagaa
      121 atagcataat tacctgcagc aaacccagat agagtctttg aagtttccca tggtaattgc
      181 acatcactga ctgattgacc attcattgtg acatgaacta atgcatatcc ttgtgacaca
      241 tttgttggtt ttgcagcagc attgattaat tggatgcttc ctgtttcaac cgttgtgctt
      301 aaataaacat tagccgttcc ctcgatatga ctatcagtga ctgccccata tttaattgaa
      361 atagaacttc caacaggtaa cttgctatta gcacccggat agctgggaaa atgcaggttt
      421 aaagtggcta gatagtttcc gccagattga ggttgagaag aaatatttaa agcattatcc
      481 gggtaagata aaggagaaaa atccccccaa aaagaggtat ttaaagctgc tgttgtttga
      541 aaggagacag ttgaattttg aaaatcgact gcctgattgc aattattggt taactttaat
      601 acgattgatt tccagtgttg atttcccgtt gctgtaaaaa gtgagtttac acaggctgta
      661 ggctgggcaa tt
//
LOCUS       DACTJE010000327          671 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.329, whole genome shotgun sequence.
ACCESSION   DACTJE010000327 DACTJE010000000
VERSION     DACTJE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.329"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..>671
                     /locus_tag="I9760_003080"
     CDS             <1..>671
                     /locus_tag="I9760_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HAT5044805.1"
                     /translation="NDEGIAEAVASKVRYLTKYQKSQILSEVIQKKKYEHAKLLIENY
                     ANLVFPSVFISVIKSRPVHIDLIRAFLNADSRHALNIDGNDLIQAAKKGDFELVKIII
                     EHRPDLLESKDSFQQTPLLWACVNGYVDIVEYLMDSGADIHVRTKVSGSHRYKIGETP
                     TGGRTALDWARCYDKTDPVKASKIIALFDTYYQRMEDHFKANSAYKDQFNKAHLTQAI
                     NNGDL"
BASE COUNT          226 a          116 c          142 g          187 t
ORIGIN      
        1 gaatgacgaa gggatagctg aagctgtggc gtcaaaagtt cgctacctga cgaaatatca
       61 aaagtcacaa atcttaagtg aagtgattca aaaaaagaaa tacgaacatg caaaactttt
      121 aattgaaaac tatgccaatc ttgttttccc atccgtgttt atttcagtga taaagagtag
      181 accggttcat attgatttga ttcgagcgtt tctcaacgca gactctcgtc atgcgttgaa
      241 tattgacggg aatgatttaa ttcaggcagc aaaaaaagga gattttgagc tggttaaaat
      301 cattatagaa catagaccag atttattgga atccaaagac tctttccaac aaactccgtt
      361 gctttgggct tgtgtcaatg gctatgtgga tatcgtggaa tatttaatgg attcaggtgc
      421 tgatattcat gtcagaacaa aagtgtctgg gagtcatcga tataaaattg gagaaacccc
      481 aactggggga cgaaccgcac ttgattgggc gcgatgctat gataaaaccg atcctgtaaa
      541 ggcatcaaag ataattgcct tatttgatac ttattatcag cgcatggaag accattttaa
      601 agccaacagt gcttataaag accaattcaa caaggctcat ttaacccagg cgataaataa
      661 tggggattta a
//
LOCUS       DACTJE010000328          588 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.330, whole genome shotgun sequence.
ACCESSION   DACTJE010000328 DACTJE010000000
VERSION     DACTJE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.330"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..>588)
                     /gene="tuf"
                     /locus_tag="I9760_003081"
     CDS             complement(<1..>588)
                     /gene="tuf"
                     /locus_tag="I9760_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911974.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAT5044806.1"
                     /translation="VETMDSYIPEPVRNIDKPFLLPIEDVFSISGRGTVVTGRVESGI
                     VKVGEEVEIVGIRDTQKTTCTGVEMFRKLLDEGRAGDNVGVLLRGTKRDEVERGQVLA
                     KPGTIKPHTKFEAEVYVLSKEEGGRHTPFFNGYRPQFYFRTTDVTGTCDLPSGVEMVM
                     PGDNVQLVVSLHAPIAMDEGLRFAIREGGRTVGAGV"
BASE COUNT          157 a          165 c           96 g          170 t
ORIGIN      
        1 tacaccggcg ccaacagttc ggccaccctc tctaattgcg aatcttaaac cttcatccat
       61 cgcaatcgga gcatgcaagc taacaactaa ttgcacatta tctccaggca ttaccatttc
      121 aactcctgat ggcaagtcac aagtacctgt cacgtcagtg gttctgaaat agaattgtgg
      181 acggtatcca ttaaagaatg gagtgtgacg tccgccttct tccttggata atacatacac
      241 ttctgcttca aacttggtgt gtggcttgat ggttcctggc ttcgccaata cctgtccacg
      301 ctccacttca tctcgcttcg tacctcgtaa taacacacca acgttatcac cagctcgacc
      361 ttcatcaagt aatttacgga acatctcaac acccgtacaa gtcgtctttt gggtgtctct
      421 tattccaaca atttcaactt cctcaccaac tttaacaatt ccactctcta cacgaccagt
      481 taccactgtt ccgcgtccag aaattgaaaa tacgtcttca atcggcaaca aaaatggctt
      541 gtctatgttt ctaactggct caggaatgta tgaatccatt gtttcaac
//
LOCUS       DACTJE010000329          584 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.331, whole genome shotgun sequence.
ACCESSION   DACTJE010000329 DACTJE010000000
VERSION     DACTJE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.331"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..>584
                     /locus_tag="I9760_003082"
     CDS             <1..>584
                     /locus_tag="I9760_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HAT5044807.1"
                     /translation="PSTRAHETLSKEQKNWFWKVVIKSWPLYSEVLVASLLVNIFALV
                     VPLFSMNVYDRVVPNHAIETMWVLASGVALVFLFDMLLKSLRSYFIDVANKHSDKELS
                     ALIFQQVLGLSMANRPKSVGSLANTVQSFEVFRDFITSTTMTVLVDLPFVFIFLFVIY
                     LIGGVLFWIPFLVLPTIFITGLLLQWPLIRLTRKS"
BASE COUNT          144 a           98 c          115 g          227 t
ORIGIN      
        1 cctagcacca gagcacatga aactctttcc aaagagcaaa aaaactggtt ttggaaagtt
       61 gttattaaat cttggccatt gtattctgaa gttcttgtgg cctccttgct cgtgaatata
      121 tttgcactcg ttgttccatt atttagcatg aatgtgtatg accgtgtggt tccaaaccat
      181 gctattgaaa ccatgtgggt gcttgctagt ggtgtagctt tggtatttct ctttgatatg
      241 ttgctcaaga gtttacgatc ctatttcatt gatgtggcaa ataaacacag tgataaagag
      301 ttgtctgctt taatatttca gcaagttctc ggattaagta tggctaacag gccaaaatcg
      361 gttggttctt tagccaatac agtacagtca tttgaagtat tcagagattt tattacttcg
      421 actactatga cagttcttgt tgatcttcct tttgtattta tttttctttt tgttatttat
      481 ttgattggcg gggtgttgtt ttggatacct tttttagtgc tccctactat ttttataaca
      541 ggtttgttgc tgcaatggcc tttaattcga cttacaagaa aatc
//
LOCUS       DACTJE010000330          525 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.332, whole genome shotgun sequence.
ACCESSION   DACTJE010000330 DACTJE010000000
VERSION     DACTJE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.332"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            200..>525
                     /locus_tag="I9760_003083"
     CDS             200..>525
                     /locus_tag="I9760_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HAT5044808.1"
                     /translation="MIHRNKVLNSILLILFTSQIAFAKSNPASQAYVDSKVSELKNEL
                     TNKINSIPSGPQGPQGPRGDKGEAGPKGDQGEAGPQGLPGPKGDRGEAGPQGLPGPKG
                     DQGEAGP"
BASE COUNT          183 a          104 c          126 g          112 t
ORIGIN      
        1 ccattttgtt aggtattgtt ttaatgattt aactaatttt tccaatagat ctcttgcata
       61 acctgcttat tttgttttag ggcaaggtac aaacgttttg aggagtagga tttacaagca
      121 gtaaatgagc accgcagaaa cgcttgcaac gcagcagtaa aataaaagag acaagtgagc
      181 ataaagtggg aaataaagaa tgatacatcg aaataaagtc cttaatagca ttttattaat
      241 cttattcaca tcacagatag catttgccaa aagcaatccg gcctcgcaag cctatgtgga
      301 cagcaaggta tctgaactaa aaaatgaatt aaccaacaaa atcaacagta tcccctctgg
      361 acctcaagga cctcaaggac ctagaggaga taaaggcgag gctggcccta aaggagacca
      421 aggagaggca gggccgcagg gattacccgg acctaaagga gaccgagggg aagcagggcc
      481 gcagggatta cccggaccta aaggagacca aggggaggca ggacc
//
LOCUS       DACTJE010000331          518 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.333, whole genome shotgun sequence.
ACCESSION   DACTJE010000331 DACTJE010000000
VERSION     DACTJE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.333"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..>518
                     /locus_tag="I9760_003084"
     CDS             <1..>518
                     /locus_tag="I9760_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAT5044809.1"
                     /translation="RKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTAKAYDQID
                     AAPEERERGITISTAHVEYESASRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAA
                     DGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSSYDFPGDD
                     IPIVVGSALKAL"
BASE COUNT          152 a           94 c          141 g          131 t
ORIGIN      
        1 cgtaagaagc cgcacgtaaa cgtgggcacg attggtcacg tagaccatgg taagacgaca
       61 ttgacagcag ctattacaac gattatggcg aagaaatatg gtggtacagc gaaggcgtac
      121 gatcaaattg atgctgcgcc agaagaaaga gagcgtggga ttactatttc tacagcacat
      181 gttgaatacg aatcagcgag cagacattat gcacacgtag actgcccggg acatgctgac
      241 tatgttaaga acatgattac tggtgctgcg caaatggacg gagcgatact ggttgtatca
      301 gcagctgatg gtcctatgcc acaaacgagg gaacacattc tattgtctcg ccaggtaggt
      361 gttccatata ttgttgtgtt catgaacaaa gcggatatgg ttgatgaccc tgagttatta
      421 gagttagtgg aaatggaagt gcgagattta ttaagcagtt acgatttccc aggggatgac
      481 atacctattg ttgttggttc agctttgaaa gcattgga
//
LOCUS       DACTJE010000332          517 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.334, whole genome shotgun sequence.
ACCESSION   DACTJE010000332 DACTJE010000000
VERSION     DACTJE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.334"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(75..320)
                     /locus_tag="I9760_003085"
     CDS             complement(75..320)
                     /locus_tag="I9760_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044810.1"
                     /translation="MNSWNSVVFLKTKNAWSDSAAIAKMNGVQSLWSTSGDWDWCIKL
                     DSKHSSPEETALFVKNLRNADWVAETKTSWWKEVAVK"
BASE COUNT          167 a          100 c           73 g          177 t
ORIGIN      
        1 cttggaatcg ctcatgacta aatgggaaat tataaaatac cattataatt tcccaaacaa
       61 ttcatcttat taaattactt tacagcaact tctttccacc aactggtttt ggtttctgca
      121 acccaatcgg catttcttaa atttttaaca aacaaagcag tttcctcagg gcttgagtgc
      181 ttggagtcaa gttttataca ccagtcccag tctccagagg tagaccaaag gctttgaaca
      241 ccattcatct ttgctattgc agcagaatct gaccaggcat ttttagtttt taaaaaaaca
      301 acactgttcc atgagttcat tgtttcttcc ttaattttaa tcaattatgt tttttcgcat
      361 tgaacataaa ttcattgcgt tatataaaat tagactatga attatcagat ttcaaactcg
      421 aatgaaaaaa aattctgata ttagaccttc tacctaagct aattattttg tttcacggca
      481 aggcgaaaac gttttgagga acagagttta catacac
//
LOCUS       DACTJE010000333          488 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.335, whole genome shotgun sequence.
ACCESSION   DACTJE010000333 DACTJE010000000
VERSION     DACTJE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.335"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..208
                     /locus_tag="I9760_003086"
     CDS             <1..208
                     /locus_tag="I9760_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HAT5044811.1"
                     /translation="NLNQYASPESRYCPAAVYEIIEDVEKGPRLQINAQNCIHCKTCD
                     IKDPRQNIVWQAPEGGGGPNYSGM"
BASE COUNT          147 a           81 c           97 g          163 t
ORIGIN      
        1 gaacttaaac caatacgctt ctccggaaag tcgatactgc ccggctgctg tttatgaaat
       61 aattgaggac gttgaaaaag gacctcgatt acaaattaac gcccagaatt gtattcattg
      121 taaaacgtgc gatattaagg atcctcgtca aaatatagtt tggcaagcac cagaaggtgg
      181 aggtggaccg aattattcag gaatgtaacc aatcaaactt aaataatatt ttcgagtaaa
      241 gtcctgtagt tattagcagg gatgttgata tcttgaacat gaaaataagg attattgcat
      301 gtaccaacct tatcttttat ttggcatttt aattctttct ctcattttat tcgtttggaa
      361 ttattagaga tacgatgcag tttgtatcct actattgatt ggtcggtact attattagct
      421 gctcttatcc ctgttggaga agctttacaa acggctgctg ctgcagattt gattgcaaat
      481 ggatttat
//
LOCUS       DACTJE010000334          458 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.336, whole genome shotgun sequence.
ACCESSION   DACTJE010000334 DACTJE010000000
VERSION     DACTJE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.336"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(<1..>458)
                     /locus_tag="I9760_003087"
     CDS             complement(<1..>458)
                     /locus_tag="I9760_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HAT5044812.1"
                     /translation="RHNQQMLRSQIMPYQNSFNKLLDELQRKAELLNKKYGTLHEATN
                     VMQALINDLSTVGNDFFMDDITPQSFQKFERKCKQVLEEASPVLSTHRGWHGYPDFVR
                     RFIGVIATLAVIPALVVHFSSAKGYVGVFFSGKENIKTDSSEKMGEFANNL"
BASE COUNT          142 a           85 c           74 g          157 t
ORIGIN      
        1 aggttattcg caaattcacc cattttttca gaagagtctg tttttatatt ttctttgcca
       61 gaaaagaaaa ctcctacata tccctttgct gatgaaaagt gtacaaccaa tgcaggaata
      121 actgccaagg ttgcaataac tcctataaat cttctgacaa aatcaggata gccatgccaa
      181 ccacgatgcg ttgataaaac tgggctggcc tcctcaagaa cctgtttaca cttcctttca
      241 aatttttgaa aagattgagg cgtaatatca tccataaaaa aatcatttcc tactgtggat
      301 aagtcattaa ttagtgcttg catgacattg gttgcttcgt gaagagtacc atatttttta
      361 ttcagtaatt ctgcttttct ttgtaattca tctaataatt tattgaatga attttgatac
      421 ggcataattt gagaacgcaa catctgttga ttatgtcg
//
LOCUS       DACTJE010000335          431 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.337, whole genome shotgun sequence.
ACCESSION   DACTJE010000335 DACTJE010000000
VERSION     DACTJE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.337"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            64..339
                     /locus_tag="I9760_003088"
     CDS             64..339
                     /locus_tag="I9760_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044813.1"
                     /translation="MKEKHFAYKITLSSTGLVCTHSEIEENRMIDLLKEIKNDDSQAI
                     FWIFKQFDKQPKEQLGIIDCTQNRIYYHYSGEVEKIDEAIEKLSKKL"
BASE COUNT          170 a           56 c           71 g          134 t
ORIGIN      
        1 tattttaaac aaagagattg ggatttctca taagtcctga tgataattaa atggagtact
       61 atcatgaaag aaaaacattt tgcctataaa attactcttt cttcaacagg gctggtttgt
      121 acccattctg aaattgaaga aaacaggatg attgatctgt taaaagaaat aaaaaacgat
      181 gattcccaag ctattttctg gatttttaaa caatttgata agcagcctaa agaacaacta
      241 gggatcatcg attgtaccca aaatcgtatt tattaccatt attcaggcga agttgaaaaa
      301 attgatgagg ctattgaaaa actaagtaaa aaattataag cctaaaagtg cccaaagaaa
      361 tatcgcattt gattgtgtgg agatattttt caaatcctgg attgaaaata tattatatga
      421 aattaaatga a
//
LOCUS       DACTJE010000336          354 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.338, whole genome shotgun sequence.
ACCESSION   DACTJE010000336 DACTJE010000000
VERSION     DACTJE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.338"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..>354
                     /locus_tag="I9760_003089"
     rRNA            <1..>354
                     /locus_tag="I9760_003089"
                     /product="23S ribosomal RNA"
BASE COUNT           92 a           80 c           97 g           85 t
ORIGIN      
        1 ggctgcgact gtttattaaa aacacagcac tctgcaaatt cgtaagaaga cgtatagggt
       61 gtgacgcctg cccggtgccg gaaggttaat tgatggggtt atcttcggag aagctcttga
      121 tcgaagcccc ggtaaacggc ggccgtaact ataacggtcc taaggtagcg aaattccttg
      181 tcgggtaagt tccgacctgc acgaatggcg taacgatggc cacactgtct ccacccgaga
      241 ctcagtgaaa ttgaaatcgc tgtgaagatg cagtgtaccc gcggctagac ggaaagaccc
      301 cgtgaacctt tactatagtt ttgcactgga ctttgatgat gactgtgtag gata
//
LOCUS       DACTJE010000337          351 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.denovo.339, whole genome shotgun sequence.
ACCESSION   DACTJE010000337 DACTJE010000000
VERSION     DACTJE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.denovo.339"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            <1..165
                     /locus_tag="I9760_003090"
     CDS             <1..165
                     /locus_tag="I9760_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VipA"
                     /protein_id="HAT5044814.1"
                     /translation="IPSMPKPNSTQPAAKNSGFFSGDGFLKELNAKIKERKERPKDEP
                     HTTPVEKKNL"
BASE COUNT          128 a           78 c           60 g           85 t
ORIGIN      
        1 ataccgtcca tgcccaaacc aaattcaacc cagcctgctg caaaaaattc aggtttcttt
       61 tcaggcgatg gttttttaaa agagttaaat gcaaaaatta aagaaagaaa ggaacgccca
      121 aaagatgagc cacataccac tcccgtcgaa aaaaaaaatc tctagttaag ctaacttctg
      181 ttcctcctga atatagggca ctcttagcta aaatggagga aattagaaat gcagtaacag
      241 gaaaatatcc tcataccgat gattcatcgc cagagtcaac tcctccttca tccccagaat
      301 taacccgttg gaagtgaggg atgaattaaa atccatagtc tactgtagaa t
//
LOCUS       DACTJE010000338         7317 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.guided.160, whole genome shotgun sequence.
ACCESSION   DACTJE010000338 DACTJE010000000
VERSION     DACTJE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.guided.160"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            complement(72..2753)
                     /gene="pepN"
                     /locus_tag="I9760_003091"
     CDS             complement(72..2753)
                     /gene="pepN"
                     /locus_tag="I9760_003091"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAT5044815.1"
                     /translation="MKTDQSKVKKLSDYKSLDYFVIHVDLQIDLSKKPVESKARLTVV
                     PNLNVDSHSNDLVLDGENMTLVSLQMNDNLLKENEYELTKDSLIIKNIPQNTPFTIEM
                     TSLLGENTDLFGLYETEGVALVKAESEGLRRVFYLPDRPDNLATYKTTIIANQEDYPV
                     LLSNGVLIEKKELPLGLHSVTWLDDVPKPSYLFALVAGNLQRSVTYYQTKSGRELPIE
                     FYVPPSATSKCDFAKEVLKEAMAWDERTFNLECALRQHMVAGVDKYASGASEPTGLNL
                     FNTENLFASPETKTDLGILRVLEVVAHEFFHYWSGDRVTIRDWFNLPLKEGLTTFRAA
                     MFREELFGTDLIRLLDGKNLDERAPRQSAYTAVRSLYTAAAYEKSADIFRMMMLFIGK
                     EPFIEAVAKFFKDNDGGAVTLEDFIESISNSSGKDLRSFLSWFTESGIPELIVTDELN
                     PDTKQYFLKIKTVNGRNRPIPILMGLLDSSGAEIVADKLLIVDQEEIEFQFENIQTRP
                     IPSLLRSFSAPVHMKYEYSYQDLLLLMQFDTNLYNRCEAAKQLISALINDFCIGKKIE
                     LSPQFFAVYKALLSDNSLNEWMLAELITLPSLEELIENQDKPDFEKLNEGRQLIQNAL
                     ANELKTDFYNLLFRIQISGDDDKQKLKGFDLKQAGLRRLKSVCFSYLLNVDFEKTKEK
                     LILQFEDALGKNMTETALALSMLCEINCEEADVALEDYYHYWKNDPGAVNNWFSIQAL
                     AHSPDVIERVKKLMRHGDFDLSNPNKVYALLGSFIKNPFGFHSVTGEGYQLVADAIFD
                     LDKINPTLAANLTEKFTYWDKYDVNRQAMMISTLKIIYSNATSSDVRTMAKKGLDKVK
                     EDLPLPIHLTFHGGSTMQDRTAQLIADGNKENAYQLH"
     gene            complement(2991..4787)
                     /gene="lem10"
                     /locus_tag="I9760_003092"
     CDS             complement(2991..4787)
                     /gene="lem10"
                     /locus_tag="I9760_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem10"
                     /protein_id="HAT5044816.1"
                     /translation="MVTKIIWVSNNGKPNLKIEFVSEEEKSNFFKEVKKKASELGLNF
                     PLVQGSGNSLLIEASNYPINPCGCYISPGGKLAINFGKVELSHFILPKVGVKTEHAEI
                     FKDHNTIFFHKHKLPGVNSELTFIPTGTPVIVPVTKPATTSPAFFNPSKSGDSSISIS
                     AIGNVDSPMIRITFQNQTEREFFLNKITDKAKSLGVNISTHPFEIKEPNMVLIKPSKY
                     PDNKLGCYISKNKEIAINFGRTDFRDFVLSNLGVGSHLGTCPTKNETGNDTFYFHQEN
                     LSLNGPALSVNTKITKEIEKNDYLLSANGSKAIDFVWSNFLKHPYNPKLKQPDANVKA
                     AWEKHADWEHWSNMGQDWSLNTPSGLVPRPNHGIAHTLRVAQLVPVIAEFLKAYSGDP
                     KFQKLTQKEIQKAQYMMLFSVIGRENDMSWTDANHYQQAFKEAYNGQYSKHIYATFKE
                     NAQKGFLNHVVTNKSSLIPSLFSDESELQYWAEALDTGKPGISSASGILMALAHDLDL
                     MRCYDKGKFNSLKMKDLVARLGGNEDAAKKLADYAHDLIVATGDRCMGYGVTQDYNYS
                     LFGKCSLDPNECLKQLQSIPKPETTLANQYGI"
     gene            complement(5123..5416)
                     /locus_tag="I9760_003093"
     CDS             complement(5123..5416)
                     /locus_tag="I9760_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAT5044817.1"
                     /translation="MMSKFFNLKTGTLHSVESYAGAVKLPKGISQESIRINERILDND
                     GYPLMAPKLRRQNANQLRMFFSRESNTLLNGSYDLADRSNRDLTEVANIISYR"
     gene            5505..5885
                     /locus_tag="I9760_003094"
     CDS             5505..5885
                     /locus_tag="I9760_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAT5044818.1"
                     /translation="MSKTYTEKLFSYGTLRYEKVQIANFGRKMQGQEDVLQGFKLTTI
                     EIKNSAVVATSGDNFHPIISYTGNPSDKVPGIVFTISKEELAQADKYEVSEYKRVNVK
                     LNSGVNAWVYINAQDLYGNNQAKS"
     gene            6262..7284
                     /gene="aph(9)-Ia"
                     /locus_tag="I9760_003095"
     CDS             6262..7284
                     /gene="aph(9)-Ia"
                     /locus_tag="I9760_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(9)-Ia"
                     /protein_id="HAT5044819.1"
                     /translation="MLRNNIPDQHLIELLKVYYGIDIHTVQLIVGGADMNAFGYKADS
                     ESNSYFVKLKYGHHDEINLSIIRLLHDSGVKEIVFPINTLEANLFQQVDHVKIIVYPF
                     IDAPNGFTKNLTEKQWKQLGKILRQIHGTSVPTSIQQRLRKETYSPKWREMVRSFYSQ
                     IRFDESDDQITTDFKSFFNQNIDSIHQLVNSSEELSKKIRPDFEKYVLCHSDIHAGNV
                     LVVDEESIYIIDWDEPMFAPKERDLMFIGGGIGNVWNKPHEIDCFYEGYGKTSIDKTI
                     LSYYRHERIVEDIAVYGHDLLSHDQNAQSRLESFKHFKSMFDPNDVIEIAFASYFNLL
                     FFRIIQ"
BASE COUNT         2365 a         1364 c         1187 g         2401 t
ORIGIN      
        1 cccccccccc cccaagtttt aggcaatatc aatatctcac tctattccaa atcaggtcat
       61 gaaataagaa atcaatgcaa ttgataagca ttttccttat ttccatcagc aattaattga
      121 gctgttctat cctgcatcgt tgatccccca tgaaaagtca aatggattgg taatgggaga
      181 tcttctttta ccttatccaa tccctttttt gccattgttc tcacatcaga tgaagtagca
      241 ttagagtaga ttatttttaa agtgctaatc atcatagctt gcctatttac atcgtattta
      301 tcccaataag taaatttttc agttagattg gcagccaaag ttggattaat tttatccaag
      361 tcaaaaatgg catcagcaac cagttggtaa ccttcacctg taacagaatg aaatccaaat
      421 gggtttttta taaatgatcc tagcagagca tacaccttgt taggattaga caaatcaaaa
      481 tcaccatgac gcattaattt ttttacccgc tctatcacat caggggagtg cgctaatgct
      541 tgaatactga accagttatt tacggcgccc ggatcatttt tccaataatg ataataatcc
      601 tctaatgcaa catccgcttc ctcacaattt atttcgcata acatgcttaa agccaaagca
      661 gtctctgtca tatttttgcc taaagcatcc tcaaattgaa gaatcagttt ctcctttgtt
      721 ttttcaaaat caacatttag caaataagaa aaacatacgg attttaaacg ccgcaatccc
      781 gcttgcttca aatcaaaacc ttttaatttt tgtttatcat catccccaga tatttgtatt
      841 ctaaaaagga gattataaaa atccgttttc aattcatttg caagggcatt ttgtattaat
      901 tgacgcccct catttaattt ttcaaaatca ggcttatcct gattttcaat caactcttct
      961 aaagacggta aggtaattaa ttcagctagc atccattcat ttaaagagtt atcagaaagt
     1021 aatgccttgt aaacagcgaa gaactgagga gataattcta tttttttccc tatacaaaaa
     1081 tcgttaatta aagctgaaat aagttgcttt gctgcctcac aacgattata caaattagtg
     1141 tcgaattgca tcaacaataa taaatcttga taagaatatt catatttcat gtgtacaggg
     1201 gcagaaaaac tacgtaataa cgatggaata gggcgggtct ggatattttc aaattgaaac
     1261 tcgatttcct cctgatcaac gattaataat ttatcagcta caatttctgc ccccgaacta
     1321 tcgagcaaac ccataaggat aggaatgggg cgatttctcc cattgactgt tttaattttt
     1381 aaaaaatatt gctttgtatc tggattcaat tcatctgtta ctatcaattc aggaattcca
     1441 gattcagtaa accaggacaa aaaagaacga agatctttac cagaagagtt gctaattgat
     1501 tcaataaaat cctccagagt aactgcacct ccatcattat ccttaaaaaa cttagctact
     1561 gcttcaataa agggttcttt acctataaac aacatcatca tacgaaaaat atcagcactt
     1621 ttttcatagg ctgcagcagt atataggctt ctaactgctg tgtaggcact ttgtcgtggt
     1681 gctctttcat ccaaattctt gccatcaagt aatcttatca gatcagtacc aaacagctcc
     1741 tctctgaaca tagcagctct aaacgtagta agaccttcct ttaatggtaa attaaaccaa
     1801 tctcttatag tgactcgatc tcctgaccaa taatgaaaaa actcatgggc aaccacttcc
     1861 aacacgcgta aaatgcctaa atcggtttta gtttcagggc ttgcaaatag attttctgta
     1921 ttaaataaat ttaatccggt tggctcggaa gcacctgacg catatttatc aaccccggct
     1981 accatatgtt gtctcaaggc acattctaaa ttaaaagtac gttcatccca tgccattgct
     2041 tctttcaata cttcttttgc aaaatcacat tttgatgtgg ctgatggtgg aacatagaat
     2101 tcgatgggta attcacggcc agattttgtt tgataataag tgactgatcg ctgtaaattg
     2161 cctgcgacca aagcaaataa ataacttggc tttgggacat catccagcca ggttacagaa
     2221 tgcagaccca aaggaagttc ttttttctca attagcacac catttgaaag tagaacagga
     2281 taatcttcct gatttgcgat gatagtggtt ttataagtcg cgagattatc aggtctgtct
     2341 ggcaaataaa atactcggcg taatccctca gactctgcct tgactaaggc cacgccctct
     2401 gtttcataca acccaaacaa atcggtattt tcaccgagca gagaggtcat ttctatagta
     2461 aagggggtat tttgaggtat gtttttaatt attaaagaat ctttagttag ctcatattca
     2521 ttttctttca ataaattatc gttcatttgt aacgatacta atgtcatatt ctcaccatcc
     2581 aataccaaat cgtttgaatg tgaatcaaca ttcaggttgg gaactactgt aagacgggct
     2641 ttggattcaa ctggtttttt gctgagatca atttgcaaat caacgtgaat tacaaaataa
     2701 tcaaggctct tataatcgct taactttttt accttggatt gatctgtttt cataaatact
     2761 ccctgtttta ccaaaaaaac taaaaattaa tattccttat ttcaactgat caaaaaccat
     2821 gttatccgct ttcagttcag tgaagtcaca atataagatc aagatagtaa tttcaatcga
     2881 gctagtgctt atttgttaga gcaatagaag caccctttgc attcaaagga tgctaattaa
     2941 ataaccgtac gatttaaact ggctacagcc atagtataaa agatttaatc tcaaatacca
     3001 tattgatttg ccaaggttgt ctctggttta ggaatacttt gaagctgctt tagacactca
     3061 ttgggatcaa ggctgcattt gccaaaaaga gagtaattat aatcctgagt taccccataa
     3121 cccatgcatc gatccccagt cgctacaatc agatcatgag cataatcagc cagtttcttt
     3181 gctgcatctt catttcctcc aagccttgca actaaatcct tcatcttcag actattaaat
     3241 tttcctttgt catagcatct catgaggtcc aaatcatgag ccagagccat aagtatgccc
     3301 gaggcagaag aaattccggg cttaccggtg tccaaagctt cagcccaata ttgaagctcg
     3361 ctttcatcgg aaaatagtga aggaattaat gacgatttat tggtaaccac atgatttaaa
     3421 aaaccttttt gagcattttc tttgaaagta gcatagatgt gcttggaata ctgtccattg
     3481 tatgcttcct taaatgcctg ctggtaatga ttggcatctg tccaactcat atcgttttca
     3541 cgcccaatta ctgaaaataa catcatgtat tgtgcttttt gtatttcttt ttgcgttaat
     3601 ttttggaatt ttggatcacc tgaatatgct ttcaaaaatt cagcaatgac tggtacaagc
     3661 tgtgcgactc gtaacgtatg agcaattcca tgatttggcc tgggaactaa acccgaggga
     3721 gtatttaatg accaatcctg tcccatattg gaccaatgtt cccaatcagc atgcttttcc
     3781 caagctgctt ttacattagc atctggctgc ttcagtttag gattataagg atgttttaaa
     3841 aaattactcc aaacaaaatc aatggccttg gagccattag cagatagtag ataatcattt
     3901 ttctctattt ctttggttat tttggtattt actgataatg cagggccatt taaagaaaga
     3961 ttctcctgat gaaaatagaa agtgtcgtta cctgtttcat tttttgttgg gcaggtccct
     4021 aaatgagagc ctactccgag attagacaac acaaagtctc taaaatctgt ccttccaaaa
     4081 ttaattgcaa tttccttatt ctttgaaata tagcaaccta atttattgtc cgggtattta
     4141 gaaggtttaa taagtaccat attaggttct ttgatttcaa aaggatgagt tgagatattg
     4201 actcctaaac tttttgcttt atctgttatt ttatttagaa aaaactctcg ctccgtttga
     4261 ttttgaaaag taattcttat catcggacta tcaacgtttc ctatcgcact aatagaaatc
     4321 gaagaatccc ctgactttga aggattaaaa aatgctggag aagtagtcgc cggttttgtt
     4381 acgggaacaa taacaggtgt gccagtcggt atgaacgtta attcagaatt cacacctgga
     4441 agtttatgtt tatggaagaa aatagtattg tggtccttga aaatctcggc atgttctgtt
     4501 ttcacgccta ctttaggtaa tataaaatga ctcaactcca cttttccaaa attaatagct
     4561 aatttcccac ctggggatat ataacaacca caaggattaa ttgggtaatt tgaagcttct
     4621 atcagaagag aatttccaga gccttgcact aatggaaaat ttagtcctaa ttcgcttgct
     4681 tttttcttta cctccttgaa aaaattactt ttttcttcct cactaacaaa ttcaattttt
     4741 aaattaggtt taccattatt agaaacccaa attattttcg taaccataat tggcctctac
     4801 tattcccagg aatatttgag aattatagta cattttgtct attaatttgt tatatggttt
     4861 tggataatga cataaaatca ggttagatga tttaacggct atgcctaata agtaatctta
     4921 gctttacatt gaagcacatg aacaagtcac ccgatttgga gccatacaac taaccataaa
     4981 atcatcagga tgaatcattc aaagcttgtt gtttatgcta tgggacttta agaataatca
     5041 gcaagtaacc aaacacattc agtgaaaaac tggtttgtca ggtatttccc tgattattat
     5101 tctggcaaat acctgattat atttagcgat aagagattat attggcaact tccgtcagat
     5161 ccctgtttga acgatcagca agatcatatg atccatttaa aagagtatta ctttctctgg
     5221 aaaaaaacat tcttaactga tttgcatttt gacgtcttaa cttaggtgcc atcaaagggt
     5281 agccgtcatt atccagaatc cgctcattta ttcttattga ctcctgactt atgcctttag
     5341 gcaatttgac tgcgccggca taggattcta cagaatgcaa ggtacctgtc tttaaattaa
     5401 aaaactttga catcatttgc tcctattggt tttataaatc tcactttgat aataataaga
     5461 aaatgattaa aattcactac ttttatttta tgagggttga ttttatgagc aaaacgtaca
     5521 cagaaaagtt attttcatac gggaccttac gctatgaaaa agtacaaatt gctaattttg
     5581 gtagaaaaat gcaaggccaa gaagatgtat tgcaaggatt caaattaacc acgattgaaa
     5641 ttaaaaattc agccgttgtt gcgacaagtg gcgataattt tcatcccatt atttcgtata
     5701 cgggcaatcc ctcagacaaa gttccaggta tcgtatttac tatcagcaaa gaagaattag
     5761 cacaagctga taaatatgaa gtctctgaat ataaacgtgt gaatgtcaaa ttgaactcag
     5821 gggtgaatgc atgggtttat atcaatgctc aagatctcta tggtaataac caagcaaaaa
     5881 gctaactagc aatagatatt agttcagaac aaatatatag agactcaaat gggtaattct
     5941 attattaatt tgtaggaaat gctttattgc caagttataa aacgaatgta atgcccatac
     6001 gttatacaat ttaaaaacta aaaaatacac atcaataatc aacgaactca tcaaggtaaa
     6061 atgtgttgtg gaagtactcc aattcaaact ttgatagagg gtaaacaaat acctcattat
     6121 acctaagaag aatagggttc attacatgcc aattaagtct gattattttg ataatcagac
     6181 ttaatattat agtaataata gtattccaat tatattattt gctttaccat tatgataatt
     6241 tccaaatagt aattaactct tatgctaaga aataacattc cggatcaaca cctcattgag
     6301 cttttgaaag tctattatgg aattgatatt catactgtac aactcatcgt gggcggtgct
     6361 gatatgaatg cctttggata taaagcagat tcagaatcca attcttattt cgtaaagcta
     6421 aaatacggcc atcatgatga aattaattta tcaatcatcc gtctcttgca tgactctgga
     6481 gtaaaagaaa ttgtttttcc tatcaacaca ctcgaagcaa acttatttca gcaagtggat
     6541 catgttaaaa taattgtgta tccatttatt gatgcgccca atggtttcac caaaaattta
     6601 acagaaaaac agtggaaaca gcttgggaaa atattaagac aaatccatgg aacctctgtt
     6661 cccacctcga tacaacagcg attaagaaaa gaaacttact cgcctaaatg gcgtgaaatg
     6721 gttaggtctt tttacagtca aattagattt gatgagtctg atgatcaaat taccactgac
     6781 ttcaaatctt tttttaatca aaatatcgac tctatccatc aattagtcaa ttcttcagaa
     6841 gaactgtcta aaaaaattcg acctgatttc gaaaaatatg tactttgtca ttctgacata
     6901 catgcaggca atgtgttggt cgttgatgag gagtccattt acattattga ttgggatgag
     6961 cccatgttcg caccaaaaga acgtgatttg atgttcattg gcggaggcat tggtaatgta
     7021 tggaataaac ctcatgaaat tgattgtttt tatgaaggtt atggtaaaac aagcatcgat
     7081 aaaaccattt tatcttatta cagacatgag cgaattgttg aagatatagc cgtgtatgga
     7141 catgacttgc tttcacatga tcaaaatgct cagtccagac ttgaaagttt taaacatttt
     7201 aagtcgatgt ttgatccaaa tgatgttatt gaaatagctt ttgcatcata ttttaatctc
     7261 ttatttttcc gcattattca atagatagat tttttatcta tccccttgat taataat
//
LOCUS       DACTJE010000339         6137 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Legionella pneumophila isolate F4901
            SAMN14214220-rid9734553.guided.178, whole genome shotgun sequence.
ACCESSION   DACTJE010000339 DACTJE010000000
VERSION     DACTJE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14214220
            Sequence Read Archive: SRR11193420
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000724086.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000724086.1
            Genome Coverage       :: 23x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/21/2020 09:08:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,101
            CDSs (total)                      :: 3,055
            Genes (coding)                    :: 3,022
            CDSs (with protein)               :: 3,022
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 3 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 16 of 33
            Pseudo Genes (incomplete)         :: 14 of 33
            Pseudo Genes (internal stop)      :: 11 of 33
            Pseudo Genes (multiple problems)  :: 6 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14214220-rid9734553.guided.178"
                     /isolate="F4901"
                     /isolation_source="environmental"
                     /db_xref="taxon:446"
                     /country="USA: New Hampshire"
                     /collection_date="2018"
     gene            2..715
                     /locus_tag="I9760_003096"
     CDS             2..715
                     /locus_tag="I9760_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT5044820.1"
                     /translation="MAIYSTPSMALSVELIETSPKSPVTLNQDEALYVLIRYKSDQPL
                     RFQAIGENLRQKIMDSARFNPSQAYPAGEGEAIAWVAYDNTTEIDSITVTIYDANWRT
                     LQTKSIPVSAVWQNENGRNNQAAAPWVQRLNQQQQSSVFTQSQTPVSSGNALFIQLLF
                     LLILLYWFLQIIVIFNWTGRWKKLACFPLLFSVPLLLYTLYALYAGSNLWPLMMLFVT
                     PFILALLLIIIGYKKVYSR"
     gene            835..2154
                     /gene="ceg23"
                     /locus_tag="I9760_003097"
     CDS             835..2154
                     /gene="ceg23"
                     /locus_tag="I9760_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg23"
                     /protein_id="HAT5044821.1"
                     /translation="MHTKKDKKVISLQERVENAVDVSGAFDNCFFHNFALYLLTNNLP
                     LPDDLFHFKSIINRNSKAEQLFEFFHNPESLNLFSILDKENDVSEPSGYLFEKSLILG
                     FLLREWFPTQLVNNSAVKAEMLEGEKGVFSAFKNYKEYRSFMSKEELKSTEFGALYEA
                     NEAFLEYFYNRSESTLINKDSPFEKYFVGSSSDEEAIKNYWDAEGYTLYCQHLAKPQV
                     KLSYIEIMTMMKVINQPLTIYDRSTSSIVAEYVNPKVNLPDFEVAIDALQGHYFLLKT
                     EETEKELEEYERSYAQYKRDRSEILAHSDKPVSSLLVRATCPKGHLDEDPFIALIESL
                     SEINSLSQIDTNLKNENTDIPNCNFLLKVGASVVGTSSALITLMGIAILSGQYDPGNS
                     LAEEALATTVVSSIVGLGYGLYSFFSSSPKVSERDSTNGLSQKLKIN"
     gene            2345..2764
                     /locus_tag="I9760_003098"
     CDS             2345..2764
                     /locus_tag="I9760_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT5044822.1"
                     /translation="MSAKKKSITHDRLLTEVELELMNIIWSLDKVTIKEVVSHLPKER
                     PLAYTTVATVLKVLEQKGFLECQKNSYAHVFTPIVTKSDYESTCIDHMVANVFDGEPR
                     ALVQRLLLARKLQHDDIQAIEEALKQLNAGEKQPEVM"
     gene            2764..4674
                     /locus_tag="I9760_003099"
     CDS             2764..4674
                     /locus_tag="I9760_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HAT5044823.1"
                     /translation="MLLIEIVLTIHCLLLLSSLFIGRAWLANKPSFKLKLLRFLLASC
                     IISPIIVHCVDSDQKPERQNYVSFDALQQYAKQSIFKTESSKFNHGSSSAFTSMNVNY
                     CQLLSVMFFLIVLFRSYHLLSGLGRLKLMLTEAIPYRTSGKLVIKVSHHCHVPFSILL
                     FNKAYIVLPVSIFSSAMNVRIAIAHEGQHHRNGDCSWAYFIEIIRIIFFGNPGVTQWF
                     RVLADLQELSCDEVLVGHQKISAHDYGHCLLQVAEAVSQCSLASNREIACTVGMALNK
                     ENEDCKFIIRRISMLTAYSPNASRPLLLGIVFAGFSILSPICVAYSTAGSLTSSRAKE
                     VDISHLDPRMQQIVEKQITTAVKQYHAKSGVIAIADPQTGNIIAFAESSKNKGLESWK
                     SRIFSPGSTIKPFIAAAAINSGNSSETKNYDCHSPYYIAGKTFTNYNSNVESASLADA
                     IAKSINVCLIRVSQEAGVPVIRKKLTEFGFDMNSWWQADQSDDLQLAMAALGENIPVT
                     IESLIKSYAILANKGHSFDRGNSAIISETSTNSINHMLENAVTNGTGKLAVIPGVSVA
                     GKTGTVIENNDKYLALFAGYVPADNPRYVLLVVIEEGYFSKNGKTLVSGGELAAPVFR
                     NVAMDALSSANR"
     gene            4724..5524
                     /gene="blaOXA"
                     /locus_tag="I9760_003100"
     CDS             4724..5524
                     /gene="blaOXA"
                     /locus_tag="I9760_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HAT5044824.1"
                     /translation="MKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQR
                     YSPASTFKIALSLMGFDSGILKDELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVW
                     YSQALTRQLGMKRFKGYVDAFHYGNQDVSGDRGQNNGLTHAWLSSSLSISPTEQIQFL
                     QKIIYKKLPVSQKAYIMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWF
                     VGWIEKDERVITFAKHIADSKENNTFASFRAKNDALIQLFNLINELEK"
     gene            complement(5649..>6137)
                     /locus_tag="I9760_003101"
     CDS             complement(5649..>6137)
                     /locus_tag="I9760_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HAT5044825.1"
                     /translation="APETISDKDITKDGRWSYINQHGKKDNPGPFYIVWDNTSTYPAG
                     WPYQVISIQIVNKKDLAFSRFLNPLHESESIKNGHHIFNNMCSTCHSIFYKGAQGRAP
                     DLGKVTSYLTPSDISKLVKHGRGYMPPIGKNLSTEEINDLIKFLIWVNRQSSKLKCEI
                     ND"
BASE COUNT         1912 a         1090 c         1195 g         1940 t
ORIGIN      
        1 cctggctatt tattcaactc catccatggc attgagtgtg gagcttatcg aaaccagtcc
       61 taaatctcct gttacattaa accaggatga ggctttgtat gtgcttattc gatataagag
      121 tgaccaacct ttgcgattcc aggctattgg ggaaaatctg cggcaaaaaa taatggatag
      181 cgccagattt aacccatcac aagcgtaccc agccggagaa ggtgaggcta ttgcctgggt
      241 agcttacgat aataccacag aaattgattc cattactgtc acaatctatg atgccaattg
      301 gaggactttg caaaccaaat caattcctgt gtctgcagtc tggcaaaatg agaatggcag
      361 aaacaatcaa gcagcagccc cctgggttca aagactgaat caacaacagc aatccagtgt
      421 ttttactcag tcacaaactc ctgtctcctc tggtaatgcc ctgtttattc aactcctttt
      481 tttactaatt cttttgtatt ggtttttgca aattattgtt atttttaatt ggacggggag
      541 atggaaaaaa ttggcgtgtt tccctttatt atttagcgta cctttgctgt tgtatacgct
      601 ttatgcgtta tatgcgggtt ctaatttatg gcctttgatg atgttatttg tgacaccatt
      661 tatcctggca cttttattga tcattatcgg gtataaaaaa gtctattcca gatgataaga
      721 tgtatccaat ttaatctatt attatatttt atctcttaat atttgcttaa taatgattct
      781 ttataatcaa aaatcaatgt aattgtttca aataacaaag agatagaata aattatgcac
      841 acaaaaaagg ataaaaaagt aatatcactt caagaaagag tagaaaatgc agttgatgtt
      901 tcgggggctt ttgataactg ctttttccat aatttcgcac tttacctgtt aacgaataat
      961 ttaccacttc ctgatgattt atttcacttc aaatcaatca ttaatagaaa tagtaaggca
     1021 gaacaattat ttgaattttt tcataacccg gaatcattga atttattttc tatattagat
     1081 aaggaaaatg atgtttctga accctctggt tatctctttg aaaagagctt gattttagga
     1141 tttctgctta gggaatggtt cccaactcaa cttgttaata attcagcagt taaagcggaa
     1201 atgctggaag gtgagaaagg ggtattcagt gcttttaaaa attataaaga gtatcgctca
     1261 tttatgtcga aggaagaatt aaaatcaacg gaatttggtg cgttatatga agcgaacgag
     1321 gcttttttag agtattttta taatcgctct gaaagtactt taataaataa ggattcacct
     1381 tttgaaaaat attttgttgg ctcgtcctca gatgaagagg caattaaaaa ttattgggac
     1441 gcagaagggt atacattata ttgtcaacat ttggcgaaac ctcaggttaa attatcttat
     1501 attgaaatta tgaccatgat gaaggtcatc aatcagccac ttacaatata tgatcgttct
     1561 acttcctcaa ttgtagccga gtatgtcaac ccaaaggtca atttacctga ttttgaagtg
     1621 gctattgatg ctcttcaagg gcattatttt cttttaaaaa cagaggaaac ggaaaaagag
     1681 ttagaagaat atgaaagaag ttatgctcaa tataaaagag atagaagtga aatccttgca
     1741 cactccgata aacctgtttc ttccttattg gttcgagcta cttgccccaa aggtcatttg
     1801 gatgaagatc cttttattgc attaattgaa agcctttccg aaataaacag ccttagccaa
     1861 attgatacta atttgaaaaa cgagaataca gatattccca attgcaactt ccttttaaag
     1921 gtaggtgctt ctgtagttgg tacctcttct gcgctaataa ctttaatggg gatagccata
     1981 ttatctggcc aatatgatcc cggaaacagt ctggctgaag aggcattagc tactactgtt
     2041 gtatccagta ttgtgggttt aggttatggg ctttatagct tctttagttc tagccccaag
     2101 gtatcagagc gtgattctac caatggatta tcgcaaaaac ttaaaataaa ctaacctcct
     2161 gaattagcaa atattgtctg taagccgatc gtgttgatat tagatatcaa cacgattgcg
     2221 ccttgagtaa actgttctca cgtgtagaga agtattttcc ccatgactat tttaactcta
     2281 ctatttttat agtaggatac tactataaaa atagtagtag cgtatgtggt tattggagag
     2341 aaacatgtcg gcaaaaaaaa aatcgataac tcatgatcga cttcttacag aagtagagct
     2401 tgaattaatg aatattatct ggagccttga taaggtaact attaaggagg ttgtttcaca
     2461 tctgcctaag gaaaggcctt tggcttatac aaccgtggca acagtactaa aagtgctgga
     2521 acaaaaggga tttcttgagt gtcaaaaaaa ttcctatgct catgttttta cacctatagt
     2581 tacaaagtca gattatgaaa gcacttgtat tgatcatatg gttgccaatg tttttgatgg
     2641 ggagccccga gcacttgtcc aaagactttt gttagccaga aagttacagc atgatgacat
     2701 tcaagccatt gaagaagcac ttaaacaatt aaacgctggt gaaaaacagc cagaggtgat
     2761 gtaatgttat taatagaaat cgtattgact attcattgcc tgcttcttct cagttcacta
     2821 tttatagggc gtgcctggct agcaaataaa ccatcattta aattgaaatt actccgtttt
     2881 ttattggcca gttgcattat ttcaccaata atagttcatt gtgtagactc tgatcaaaag
     2941 cctgaacgac aaaactatgt atcatttgat gctttgcaac agtatgcaaa gcaatccata
     3001 tttaagacag aatcttcaaa atttaatcat ggatcgtctt ctgcctttac atcaatgaat
     3061 gtgaactact gtcaactctt aagcgtgatg ttttttttaa tcgtcttgtt tcgtagttat
     3121 cacttgttat caggtttagg tcgcttgaaa ttgatgttga ctgaagcaat cccatatcga
     3181 acttccggaa aattagttat taaggtttct caccactgcc atgttccttt ctcaatattg
     3241 ctatttaata aagcctacat cgtattgcca gtatccatat tttcttctgc catgaatgtg
     3301 aggattgcta ttgctcatga agggcaacac catcgtaatg gagattgttc atgggcctat
     3361 tttatcgaaa tcatacgcat tatcttcttt ggaaacccgg gtgtaactca atggtttcgt
     3421 gttttagcag atttacagga attgtcatgt gatgaagtat tggttggaca tcaaaaaatt
     3481 tcggcacatg actatggtca ctgtctattg caagtggcag aggcagtatc ccaatgctct
     3541 ctggcatcta accgggaaat tgcatgcacg gtgggtatgg ctttgaacaa agaaaacgaa
     3601 gactgcaaat ttataataag gagaatttca atgctaactg cttattcacc caatgcatcc
     3661 agacctttgc tattagggat agtctttgca ggattttcta tcctgtctcc aatctgcgtt
     3721 gcctattcaa cagcaggatc tttgacgagt tccagggcaa aagaagttga tatttctcat
     3781 ttagatccca gaatgcagca gattgtggag aaacaaatta cgacagccgt caaacaatat
     3841 catgcaaaat ctggagtaat tgcaatagct gatccccaaa caggcaatat cattgcattt
     3901 gctgaatcaa gtaaaaataa ggggctagag agctggaaat cacgtatttt ttcacccgga
     3961 tctactataa aaccttttat tgcagcagct gcaatcaact ctgggaatag ctctgaaaca
     4021 aaaaattatg attgtcattc accttattac atagcaggca aaacatttac aaattataat
     4081 tccaatgttg aatcagcttc cttagcagat gccatcgcaa aatctatcaa tgtatgcctt
     4141 atcagagttt ctcaagaggc tggagtgccg gtaatccgta aaaagctcac tgaatttggt
     4201 tttgacatga acagttggtg gcaagcggat caaagtgacg atttacaact ggcaatggct
     4261 gcacttggag agaatatccc tgtcacaatc gaatcgctaa taaaatctta tgccattctt
     4321 gctaacaaag gacattcatt tgacagggga aatagtgcca ttatttcaga aacaagcact
     4381 aactcaataa atcatatgct tgaaaatgct gttacaaatg gcactggaaa actcgcagtt
     4441 attcctggtg tttctgtagc tggtaagaca ggaactgtaa ttgaaaataa tgataaatat
     4501 cttgctttgt ttgccgggta tgtgccagct gataatccgc gttatgtttt attagtcgtg
     4561 attgaagagg gttatttcag taaaaatgga aagacattag tttcgggagg agaattggcc
     4621 gcaccggtat tccgaaatgt cgcaatggat gctctgagca gtgcaaatcg ataactagct
     4681 tatgggtttg catatccata tattcaaaaa tgttgaggtt gttatgaaga aaataattat
     4741 atttttgtgt acaggattat tattttgcgt ctctacttgg gctcaaggca cctgtttttt
     4801 agcaaaagaa aatcaaactg ttttaaaacg tgagggcaat gattgcgatc aacgctattc
     4861 gccagcatca acttttaaaa ttgcccttag cctcatgggc tttgattctg gaatattaaa
     4921 ggatgagttg catcctgaat ggccatacaa aaaagaatat gaactttatc tgaatgtatg
     4981 gaagtaccct caaaacccgc atacctggat cagagattcc tgtgtttggt attcacaggc
     5041 attaactcga caattgggca tgaaacggtt taaagggtat gtcgacgcat ttcactacgg
     5101 taatcaggat gtttcgggag acagaggtca aaataacggc ttaacccatg cctggctttc
     5161 cagctcactt tctatctcac ccacagagca aattcaattt ctacaaaaaa taatctacaa
     5221 gaaactaccg gtgagtcaaa aagcttatat catgactaaa aatatcatgt acatccagga
     5281 gttgcctggt gggtggaagc tttatggaaa aacaggaact ggtcggcagt taacaaaaga
     5341 taaaagccaa aaattgcctt tgcaacatgg atggttcgta ggttggattg agaaggatga
     5401 gcgtgtaatt acctttgcaa agcatattgc agatagtaaa gagaataaca cttttgccag
     5461 ttttagagcg aaaaatgatg ctctgattca attgtttaac ctcattaatg agttggagaa
     5521 ataatctaag acattgtcgg agcaggatta ttctgttccg attacccaaa tctgccaact
     5581 atagtcacac acattccagg agttgctgct gcttgtcatc ggtagctaca aagtcctaaa
     5641 ttatatcctt agtcattaat ttcgcatttt agtttggatg attgtctgtt aacccagatt
     5701 aaaaatttaa tgagatcatt aatttcttcc gtagagaggt ttttgcctat cggtggcatg
     5761 tatcctcgtc catgctttac aagcttactg atatcagaag gtgttaagta gctcgtcact
     5821 ttgcctaagt ctggtgcgcg tccttgagca cccttgtaaa atatagagtg gcaagtggag
     5881 cacatattat taaaaatatg atgaccattt tttatagact ctgattcatg caaaggattt
     5941 aagaaacgag aaaaagcaag gtctttttta ttcactattt gaatcgaaat aacctggtat
     6001 ggccaaccgg cagggtaggt gcttgtatta tcccatacaa tatagaaagg gccgggatta
     6061 tcttttttac cgtgttgatt aatataactc cagcgaccat ctttggtgat gtccttgtca
     6121 gaaattgttt cgggcgc
//
